title: Site-selected incorporation of 5-carboxymethylaminomethyl(-2-thio)uridine into RNA sequences by phosphoramidite chemistry.
abstract: 5-Carboxymethylaminomethyluridine (cmnm(5)U) and 5-carboxymethylaminomethyl-2-thiouridine (cmnm(5)s(2)U) are located at the wobble position in several cytosolic and mitochondrial tRNA sequences. In this paper, we report the first site-selected incorporation of cmnm(5)U and cmnm(5)s(2)U into RNA sequences by phosphoramidite chemistry on a CPG solid support. Trifluoroacetyl and 2-(trimethylsilyl)ethyl were selected for the protection of the amine and carboxyl functions, respectively.
authors.affiliation: Institute of Organic Chemistry, Zeromskiego St. 116, Lodz, Poland. grazyna.leszczynska@p.lodz.pl.
authors.forename: Grazyna
authors.initials: G
authors.lastname: Leszczynska
authors.forename: Jakub
authors.initials: J
authors.lastname: Pięta
authors.forename: Karolina
authors.initials: K
authors.lastname: Wozniak
authors.forename: Andrzej
authors.initials: A
authors.lastname: Malkiewicz
mesh_terms: D001483:Base Sequence; D005998:Glycine / Q000031:analogs & derivatives* / Q000737:chemistry; D015394:Molecular Structure; D009943:Organophosphorus Compounds / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D013891:Thiouridine / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D009943:Organophosphorus Compounds; C434331:phosphoramidite; D013891:Thiouridine; D012313:RNA; D005998:Glycine