title: Novel antiplasmodial agents from Christia vespertilionis.
abstract: The roots, leaves and stems of Christia vespertilionis were separately and successively extracted with methanol and aqueous-methanol (1:4, v/v) and were evaluated in vitro for their antiplasmodial potential against Plasmodium falciparum NF-54. The aqueous-methanolic stem (AS) extract was the most active (IC50 7.5 microg/mL) followed by the methanolic leaf (ML) extract (IC50 32.0 microg/mL). The in vivo antimalarial activity of the combined plant extract of C. vespertilionis was also assessed in P. berghei infected mice, which showed 87.8% suppression of parasitaemia as compared with complete suppression by chloroquine on day 8. Finally, detailed chemical investigation of C. vespertilionis resulted in the isolation and characterization of fifteen compounds (1-15), of which two (1 and 4) are being reported for the first time from nature. The novel compound 1 possesses potent antiplasmodial activity (IC50 = 9.0 microg/mL).
authors.affiliation: Medicinal Chemistry Department, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), P.O.- CIMAP, Lucknow-226015, India.
authors.forename: Harish C
authors.initials: HC
authors.lastname: Upadhyay
authors.affiliation: Molecular Bioprospection Department, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), P.O.- CIMAP, Lucknow-226015, India.
authors.forename: Brijesh S
authors.initials: BS
authors.lastname: Sisodia
authors.affiliation: Molecular Bioprospection Department, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), P.O.- CIMAP, Lucknow-226015, India.
authors.forename: Harveer S
authors.initials: HS
authors.lastname: Cheema
authors.affiliation: Molecular Bioprospection Department, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), P.O.- CIMAP, Lucknow-226015, India.
authors.forename: Jyoti
authors.initials: J
authors.lastname: Agrawal
authors.affiliation: Molecular Bioprospection Department, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), P.O.- CIMAP, Lucknow-226015, India.
authors.forename: Anirban
authors.initials: A
authors.lastname: Pal
authors.affiliation: Molecular Bioprospection Department, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), P.O.- CIMAP, Lucknow-226015, India.
authors.forename: Mahendra P
authors.initials: MP
authors.lastname: Darokar
authors.affiliation: Medicinal Chemistry Department, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), P.O.- CIMAP, Lucknow-226015, India.
authors.forename: Santosh K
authors.initials: SK
authors.lastname: Srivastava
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000302:isolation & purification* / Q000494:pharmacology; D007887:Fabaceae / Q000737:chemistry*; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000962:Antimalarials; D010936:Plant Extracts