title: [Analysis on preparation and characterization of asiaticoside-loaded flexible nanoliposomes].
abstract: Asiaticoside is a compound extracted from traditional Chinese medicine Centella asiatica, and mainly used in wound healing and scar repair in clinical, with notable efficacy. However, its poor transdermal absorption and short action time restrict its wide application. In this experiment, the reserve-phase-extrusion-lyophilization method was conducted to prepare the lyophilized asiaticoside-loaded flexible nanoliposomes (LAFL). Its characteristics including electron microscope structure, particle size, Zeta potential, entrapment rate, drug-loading rate, stability and drug release were determined with the intelligent transdermal absorption instrument. LAFL were white spheroids, with pH, particle size and zeta potential of 7. 03, 70. 14 nm and - 36. 5 mV, respectively. The average entrapment rate of the 3 batch samples were 31. 43% , and the average asiaticoside content in 1 mg lyophilized simple was 0. 134 mg. The results indicated that LAFL have good physicochemical properties and pharmaceutical characteristics, with an improved transdermal performance.
authors.affiliation: Engineering Research Center of Bioreactor and Pharmaceutical Development under Ministry of Education, Jilin Agricultural University, Changchun 130118, China. 9877699@qq.com
authors.forename: Yan
authors.initials: Y
authors.lastname: Ren
authors.affiliation: Suzhou Sciscape Biomed Scitech Co., Ltd., Suzhou 215125, China.
authors.forename: Xing-Dong
authors.initials: XD
authors.lastname: He
authors.affiliation: Suzhou Sciscape Biomed Scitech Co., Ltd., Suzhou 215125, China.
authors.forename: Bei-Cheng
authors.initials: BC
authors.lastname: Shang
authors.affiliation: Engineering Research Center of Bioreactor and Pharmaceutical Development under Ministry of Education, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Xiu-Kun
authors.initials: XK
authors.lastname: Bao
authors.affiliation: Engineering Research Center of Bioreactor and Pharmaceutical Development under Ministry of Education, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Wang
authors.affiliation: Engineering Research Center of Bioreactor and Pharmaceutical Development under Ministry of Education, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Ji-Sheng
authors.initials: JS
authors.lastname: Ma
mesh_terms: D000818:Animals; D028041:Centella; D008081:Liposomes / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010936:Plant Extracts; D014315:Triterpenes / Q000737:chemistry*
chemical_list: C526146:Centella asiatica extract; D008081:Liposomes; D010936:Plant Extracts; D014315:Triterpenes; C004446:asiaticoside