title: Primary recovery of a bacteriocin-like inhibitory substance derived from Pediococcus acidilactici Kp10 by an aqueous two-phase system.
abstract: A polymer-salt aqueous two-phase system (ATPS) consisting of polyethylene-glycol (PEG) with sodium citrate was developed for direct recovery of a bacteriocin-like inhibitory substance (BLIS) from a culture of Pediococcus acidilactici Kp10. The influences of phase composition, tie-line length (TLL), volume ratio (VR), crude sample loading, pH and sodium chloride (NaCl) on the partition behaviour of BLIS was investigated. Under optimum conditions of ATPS, the purification of BLIS was achieved at 26.5% PEG (8000)/11% sodium citrate with a TLL of 46.38% (w/w), VR of 1.8, and 1.8% crude load at pH 7 without the presence of NaCl. BLIS from P. acidilactici Kp10 was successfully purified by the ATPS up to 8.43-fold with a yield of 81.18%. Given that the operation of ATPS is simple, environmentally friendly and cost-effective, as it requires only salts and PEG, it may have potential for industrial applications in the recovery of BLIS from fermentation broth.
authors.affiliation: Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Sahar
authors.initials: S
authors.lastname: Abbasiliasi
authors.affiliation: Institute of Bioscience, Universiti Putra Malaysia, 43300 Serdang, Selangor, Malaysia.
authors.forename: Joo Shun
authors.initials: JS
authors.lastname: Tan
authors.affiliation: Institute of Bioscience, Universiti Putra Malaysia, 43300 Serdang, Selangor, Malaysia; Department of Veterinary Preclinical Sciences, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Tengku Azmi Tengku
authors.initials: TA
authors.lastname: Ibrahim
authors.affiliation: Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Saeid
authors.initials: S
authors.lastname: Kadkhodaei
authors.affiliation: Department of Food Science and Nutrition, Faculty of Applied Sciences, UCSI University, Cheras, 56000 Kuala Lumpur, Malaysia.
authors.forename: Hui Suan
authors.initials: HS
authors.lastname: Ng
authors.affiliation: Institute of Bioscience, Universiti Putra Malaysia, 43300 Serdang, Selangor, Malaysia.
authors.forename: Faezeh
authors.initials: F
authors.lastname: Vakhshiteh
authors.affiliation: Innovation Center for Confectionery Technology (MANIS), Faculty of Science and Technology, University Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Ajdari
authors.affiliation: Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Shuhaimi
authors.initials: S
authors.lastname: Mustafa
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia.
authors.forename: Tau Chuan
authors.initials: TC
authors.lastname: Ling
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Raha Abdul
authors.initials: RA
authors.lastname: Rahim
authors.affiliation: Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. Electronic address: arbarif@upm.edu.my.
authors.forename: Arbakariya B
authors.initials: AB
authors.lastname: Ariff
mesh_terms: D001430:Bacteriocins / Q000032:analysis*; D010376:Pediococcus / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry; D014867:Water
chemical_list: D001430:Bacteriocins; D014867:Water; D011092:Polyethylene Glycols; D012965:Sodium Chloride
keywords: Aqueous two-phase system; Bacteriocin-like inhibitory substance; Pediococcus acidilactici; Recovery