title: Recovery of Hafnia alvei from diseased brown trout, Salmo trutta L., and healthy noble crayfish, Astacus astacus (L.), in Bulgaria.
abstract: Hafnia alvei was isolated in Bulgaria from healthy noble crayfish, Astacus astacus (L.), and then from farmed diseased brown trout, Salmo trutta L., with signs of haemorrhagic septicaemia. The isolates were identified initially with conventional phenotyping and commercial Merlin Micronaut and API 20E rapid identification systems, followed by sequencing of the 16S rRNA gene. Hafnia alvei Bt1, Bt2 and Aa4 were of low virulence to rainbow trout and brown trout, although cytotoxicity was demonstrated by Bt1 and Bt2, but not by Aa4.
authors.affiliation: National Reference Laboratory for Fish, Molluscs and Crustacean Diseases, National Diagnostic Science-and-Research Veterinary Medical Institute, Sofia, Bulgaria.
authors.forename: P
authors.initials: P
authors.lastname: Orozova
authors.forename: I
authors.initials: I
authors.lastname: Sirakov
authors.forename: V
authors.initials: V
authors.lastname: Chikova
authors.forename: R
authors.initials: R
authors.lastname: Popova
authors.forename: A H
authors.initials: AH
authors.lastname: Al-Harbi
authors.forename: M
authors.initials: M
authors.lastname: Crumlish
authors.forename: B
authors.initials: B
authors.lastname: Austin
mesh_terms: D000818:Animals; D003400:Astacoidea / Q000469:parasitology*; D002031:Bulgaria; D005798:Genes, Bacterial / Q000235:genetics; D020611:Hafnia alvei / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity / Q000502:physiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014337:Trout / Q000469:parasitology*
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Hafnia alvei; brown trout Salmo trutta L.; fish disease; virulence