title: Comparative proteomic analysis of malignant pleural mesothelioma evidences an altered expression of nuclear lamin and filament-related proteins.
abstract: OBJECTIVE
  Malignant mesothelioma is a neoplastic disease linked to asbestos exposure whose diagnosis is limited, so detection methods for an early diagnosis and treatment result essential. Here, we compared proteomic profiles of malignant pleural mesothelioma (MPM) and benign biopsies to search potential biomarkers useful in differential diagnosis.
  
  
  METHODS
  Tissue biopsies were obtained from 53 patients who were subjected to a diagnostic thoracoscopy. 2DE/MS based approach was used for proteomic analysis and protein validation was carried out by Western blot analysis versus benign and lung carcinoma samples.
  
  
  RESULTS
  Among the proteins identified we confirmed known MPM biomarkers such as calretinin and suggested the new ones as prelamin A/C, desmin, vimentin, calretinin, fructose-bisphosphate aldolase A, myosin regulatory light chain 2, ventricular/cardiac muscle isoform, myosin light chain 3 and myosin light chain 6B. Ingenuity software was used to identify the biological processes to which these proteins belong and to construct a potential network.
  
  
  CONCLUSIONS
  Overall, our results suggest potential biomarkers that can be useful in occupational medicine for the early identification of the onset of disease in health surveillance of past asbestos-exposed workers, for monitoring the progress of disease and for assessing the response to treatment.
authors.affiliation: Department of Pharmacy, University of Pisa, Pisa, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Giusti
authors.forename: Ylenia
authors.initials: Y
authors.lastname: Da Valle
authors.forename: Alessandra
authors.initials: A
authors.lastname: Bonotti
authors.forename: Elena
authors.initials: E
authors.lastname: Donadio
authors.forename: Federica
authors.initials: F
authors.lastname: Ciregia
authors.forename: Tiziana
authors.initials: T
authors.lastname: Ventroni
authors.forename: Rudy
authors.initials: R
authors.lastname: Foddis
authors.forename: Gino
authors.initials: G
authors.lastname: Giannaccini
authors.forename: Giovanni
authors.initials: G
authors.lastname: Guglielmi
authors.forename: Alfonso
authors.initials: A
authors.lastname: Cristaudo
authors.forename: Antonio
authors.initials: A
authors.lastname: Lucacchini
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001194:Asbestos / Q000633:toxicity; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D006801:Humans; D034921:Lamin Type B / Q000096:biosynthesis; D008175:Lung Neoplasms / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D008297:Male; D008654:Mesothelioma / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D000086002:Mesothelioma, Malignant; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis; D010997:Pleural Neoplasms / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D040901:Proteomics
chemical_list: D014408:Biomarkers, Tumor; D034921:Lamin Type B; D009363:Neoplasm Proteins; D001194:Asbestos
keywords: Biomarkers; Lung; Mesothelioma; Prelamin A/C