title: Molecular characterization of multidrug-resistant Mycobacterium tuberculosis isolates from China.
abstract: To investigate the molecular characterization of multidrug-resistant tuberculosis (MDR-TB) isolates from China and the association of specific mutations conferring drug resistance with strains of different genotypes, we performed spoligotyping and sequenced nine loci (katG, inhA, the oxyR-ahpC intergenic region, rpoB, tlyA, eis, rrs, gyrA, and gyrB) for 128 MDR-TB isolates. Our results showed that 108 isolates (84.4%) were Beijing family strains, 64 (59.3%) of which were identified as modern Beijing strains. Compared with the phenotypic data, the sensitivity and specificity of DNA sequencing were 89.1% and 100.0%, respectively, for isoniazid (INH) resistance, 93.8% and 100.0% for rifampin (RIF) resistance, 60.0% and 99.4% for capreomycin (CAP) resistance, 84.6% and 99.4% for kanamycin (KAN) resistance, and 90.0% and 100.0% for ofloxacin (OFX) resistance. The most prevalent mutations among the MDR-TB isolates were katG315, inhA15, rpoB531, -526, and -516, rrs1401, eis-10, and gyrA94, -90, and -91. Furthermore, there was no association between specific resistance-conferring mutations and the strain genotype. These findings will be helpful for the establishment of rapid molecular diagnostic methods to be implemented in China.
authors.affiliation: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention/State Key Laboratory for Infectious Disease Prevention and Control, Beijing, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Zhao
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Hai-Can
authors.initials: HC
authors.lastname: Liu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xia
authors.forename: Xiao-Cui
authors.initials: XC
authors.lastname: Wu
authors.forename: Qing
authors.initials: Q
authors.lastname: Sun
authors.forename: Xiu-Qin
authors.initials: XQ
authors.lastname: Zhao
authors.forename: Gui-Lian
authors.initials: GL
authors.lastname: Li
authors.forename: Zhi-Guang
authors.initials: ZG
authors.lastname: Liu
authors.forename: Kang-Lin
authors.initials: KL
authors.lastname: Wan
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002207:Capreomycin / Q000494:pharmacology; D005838:Genotype; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology; D007612:Kanamycin / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000378:metabolism; D015242:Ofloxacin / Q000494:pharmacology; D012293:Rifampin / Q000494:pharmacology; D018088:Tuberculosis, Multidrug-Resistant / Q000235:genetics / Q000382:microbiology*
chemical_list: D000995:Antitubercular Agents; D001426:Bacterial Proteins; D002207:Capreomycin; D007612:Kanamycin; D015242:Ofloxacin; D007538:Isoniazid; D012293:Rifampin