title: Recent trends in clinically significant nontuberculous Mycobacteria isolates at a Korean general hospital.
abstract: Lung disease caused by nontuberculous mycobacteria (NTM) represents an increasing proportion of all mycobacterial diseases. We investigated recent occurrences of NTM and evaluated the clinical significance of NTM isolates from 752 respiratory specimens collected from patients at National Health Insurance Service Ilsan Hospital between January 2007 and May 2011. Specimens were incubated on solid and liquid media (BACTEC MGIT 960, BD, USA) for 6-8 weeks, and PCR and reverse blot hybridization were performed (REBA Myco-ID, Molecules & Diagnostics, Korea). Clinical features of the patients were reviewed through medical records. The most frequently isolated organism was Mycobacterium avium (46.7%), followed by M. intracellulare (14.8%), M. fortuitum (7.2%), and M. abscessus (6.6%). The most common mycobacteria among definitive cases of NTM lung disease were M. avium (42/351, 12.0%), M. intracellulare (19/111, 17.1%), M. abscessus (11/50, 22.0%), M. massiliense (4/13, 30.8%), and M. fortuitum (4/54, 7.4%). Clinically significant cases of NTM lung disease increased from 4 patients in 2007 to 32 in 2011. The mean patient age was 64 yr (range: 35-88 yr), and 58 (64%) patients were women. Patients suffered from cough, productive sputum, and hemoptysis. In summary, the most common mycobacteria causing NTM lung disease were M. avium and M. intracellulare; however, cases of M. massiliense and M. abscessus infection are on the rise in Korea.
authors.affiliation: Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Hyun Soo
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea. ; Department of Laboratory Medicine, National Health Insurance Service Ilsan Hospital, Goyang, Korea.
authors.forename: Yangsoon
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Department of Laboratory Medicine, National Health Insurance Service Ilsan Hospital, Goyang, Korea.
authors.forename: Sangsun
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Laboratory Medicine, National Health Insurance Service Ilsan Hospital, Goyang, Korea.
authors.forename: Young Ah
authors.initials: YA
authors.lastname: Kim
authors.affiliation: Department of Laboratory Medicine, National Health Insurance Service Ilsan Hospital, Goyang, Korea.
authors.forename: Young-Kyu
authors.initials: YK
authors.lastname: Sun
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006769:Hospitals, General / Q000592:standards / Q000639:trends*; D006801:Humans; D008171:Lung Diseases / Q000175:diagnosis / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D009165:Mycobacterium Infections, Nontuberculous / Q000175:diagnosis / Q000382:microbiology*; D009170:Nontuberculous Mycobacteria / Q000235:genetics / Q000502:physiology*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic; D056910:Republic of Korea; D013183:Sputum / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic
keywords: Lung diseases; M. avium; M. massiliense; Nontuberculous mycobacterium