title: Short communication: Protoplast formation from the thermophilic fungus Malbranchea sulfurea, using the thermostable chitinase and laminarinase of Paecilomyces varioti.
abstract: Two thermostable enzymes produced by the thermophilic fungus Paecilomyces varioti, a chitinase and laminarinase, were used to isolate protoplasts of a thermophilic fungus, Malbranchea sulfurea. The frequency of protoplast regeneration observed (35%) was considerably higher than that obtained using commercial lytic enzymes.
authors.affiliation: Department of Biological Science, Rani Durgavati University, MP - 482 001, Jabalpur, India.
authors.forename: S P
authors.initials: SP
authors.lastname: Gautam
authors.forename: A K
authors.initials: AK
authors.lastname: Gupta
authors.forename: R
authors.initials: R
authors.lastname: Shrivastava
authors.forename: M
authors.initials: M
authors.lastname: Awasthi