title: In vitro propagation of Homalomena aromatica Schott., an endangered aromatic medicinal herb of Northeast India.
abstract: A successful report on the in vitro propagation of Homalomena aromatica via rhizome axillary bud multiplication is presented. Rhizome bud explants were cultured on Murashige and Skoog medium supplemented with various concentrations of cytokinins to induce multiple shoot formation for micropropagation. The highest number of shoots was achieved in MS medium supplemented with 2.0 mg l(-1) 6-benzylaminopurine. The regenerated shoots rooted most efficiently on half-strength MS medium supplemented with 0.5 mg l(-1) α-naphthalene acetic acid. The regenerated plantlets showed no morphological differences from the parent plant. This protocol takes approximately 6 months to reach the acclimatization stage from the initiation stage and facilitates commercial and rapid propagation of H. aromatica.
authors.affiliation: Plant Biotechnology Laboratory, Centre for Advanced Studies in Botany, North-Eastern Hill University, Shillong, 793022 India.
authors.forename: Shiveirou
authors.initials: S
authors.lastname: Raomai
authors.affiliation: Plant Biotechnology Laboratory, Centre for Advanced Studies in Botany, North-Eastern Hill University, Shillong, 793022 India.
authors.forename: Suman
authors.initials: S
authors.lastname: Kumaria
authors.affiliation: Plant Biotechnology Laboratory, Centre for Advanced Studies in Botany, North-Eastern Hill University, Shillong, 793022 India.
authors.forename: Pramod
authors.initials: P
authors.lastname: Tandon
keywords: Cytokinins; Homalomena aromatica; In vitro propagation; Multiple shoots; Rhizome axillary bud