title: Partial purification and some properties of an alkaline cellulase from an alkalophilic Bacillus sp.
abstract: A newly isolated Bacillus species, which grew optimally at 30°C and pH 10, produced a carboxymethylcellulase in a medium containing 10 g CM-cellulose/l. The enzyme, when partially purified by gel filtration, had a mass of about 29 kDa as determined by both SDS-PAGE and gel filtration chromatography. It was optimally active at pH 9.5 and 40°C, and was stable from pH 7 to 11 at 4°C for 24 h. The enzyme was stimulated by Ca(2+) (1mM) but was completely inhibited by Hg(2+) (1MM). Neither EDTA nor EGTA (10MM) affected the activity.
authors.affiliation: , .
authors.forename: H
authors.initials: H
authors.lastname: Khyami-Horani