title: Clusterin expression level correlates with increased oxidative stress in asthmatics.
abstract: BACKGROUND
  Oxidative stress is thought to play a role in the pathogenesis of asthma. Clusterin is a sensitive cellular biosensor of oxidative stress and has antioxidant properties. The function and expression of clusterin in patients with asthma have not been fully investigated.
  
  
  OBJECTIVE
  To investigate whether the expression of clusterin in patients with asthma is regulated by increased oxidative burden and whether clusterin expression could be used to assess the response to inhaled corticosteroids.
  
  
  METHODS
  Clusterin levels in serum, induced sputum, and peripheral blood mononuclear cells of patients with asthma were measured by enzyme-linked immunosorbent assay and western blotting and compared with pulmonary function and levels of expression of hyperoxidized peroxiredoxins. Serum concentrations of clusterin in treatment-naive patients were compared before and after inhaled corticosteroid use.
  
  
  RESULTS
  Serum clusterin concentration was significantly elevated in patients with severe asthma and was inversely correlated with pulmonary function. The expression of hyperoxidized peroxiredoxins was greatly increased in peripheral blood mononuclear cells of patients with asthma and was strongly correlated with clusterin expression. Serum clusterin concentrations in treatment-naive patients with asthma were decreased significantly after initial treatment with inhaled corticosteroids.
  
  
  CONCLUSIONS
  Clusterin may be a biomarker of asthma severity and the burden of oxidative stress in patients with asthma. Moreover, clusterin may be useful for the prompt assessment of airway inflammation.
authors.affiliation: Department of Allergy and Clinical Immunology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.
authors.forename: Hyouk-Soo
authors.initials: HS
authors.lastname: Kwon
authors.affiliation: Department of Allergy and Clinical Immunology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.
authors.forename: Tae-Bum
authors.initials: TB
authors.lastname: Kim
authors.affiliation: Department of Allergy and Clinical Immunology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.
authors.forename: Yoon Su
authors.initials: YS
authors.lastname: Lee
authors.affiliation: Department of Medicine, University of Ulsan College of Medicine, Seoul, Republic of Korea.
authors.forename: Seung-Hwan
authors.initials: SH
authors.lastname: Jeong
authors.affiliation: Asan Medical Center, Health Screening and Promotion Center, Seoul, Republic of Korea.
authors.forename: Yun-Jeong
authors.initials: YJ
authors.lastname: Bae
authors.affiliation: Asan Institute of Life Science, Seoul, Republic of Korea.
authors.forename: Keun-Ai
authors.initials: KA
authors.lastname: Moon
authors.affiliation: Asan Institute of Life Science, Seoul, Republic of Korea.
authors.forename: Bo-Ram
authors.initials: BR
authors.lastname: Bang
authors.affiliation: Department of Allergy and Clinical Immunology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.
authors.forename: Hee-Bom
authors.initials: HB
authors.lastname: Moon
authors.affiliation: Department of Allergy and Clinical Immunology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea. Electronic address: yscho@amc.seoul.kr.
authors.forename: You Sook
authors.initials: YS
authors.lastname: Cho
mesh_terms: D000305:Adrenal Cortex Hormones / Q000627:therapeutic use; D018927:Anti-Asthmatic Agents / Q000627:therapeutic use; D001249:Asthma / Q000188:drug therapy / Q000276:immunology / Q000378:metabolism*; D001507:Beclomethasone / Q000627:therapeutic use; D015415:Biomarkers / Q000097:blood; D051152:Clusterin / Q000096:biosynthesis / Q000097:blood* / Q000378:metabolism; D005260:Female; D005541:Forced Expiratory Volume; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D054464:Peroxiredoxins / Q000097:blood / Q000378:metabolism; D012129:Respiratory Function Tests; D013183:Sputum / Q000378:metabolism
chemical_list: D000305:Adrenal Cortex Hormones; D018927:Anti-Asthmatic Agents; D015415:Biomarkers; D051152:Clusterin; D054464:Peroxiredoxins; D001507:Beclomethasone