title: Comparative analysis of mRNA expression of surface antigens between histiocytic and nonhistiocytic sarcoma in dogs.
abstract: BACKGROUND
  Definitive diagnosis of histiocytic sarcoma (HS) in dogs is relatively difficult by conventional histopathological examination because objective features of HS are not well defined.
  
  
  OBJECTIVE
  Quantitative analysis of mRNA expression of selected cellular surface antigens (SAs) specific to HS in dogs can facilitate objective and rapid diagnosis.
  
  
  METHODS
  Dogs with HS (n = 30) and dogs without HS (n = 36), including those with other forms of lymphoma (n = 4), inflammatory diseases (n = 6), and other malignant neoplasias (n = 26).
  
  
  METHODS
  Retrospective clinical observational study. Specimens were collected by excisional biopsy, needle core biopsy, or fine needle aspiration. To determine HS detection efficacy, mRNA expression levels of selected SAs specific to HS in dogs, including MHC class IIα, CD11b, CD11c, and CD86, were quantitatively analyzed using real-time quantitative polymerase chain reaction.
  
  
  RESULTS
  Each SA mRNA expression level was significantly higher in HS dogs than in non-HS dogs (P = .0082). Cutoff values for discriminating between HS and non-HS dogs based on these expression levels were calculated on the basis of receiver-operating characteristic analysis. Accuracy of the cutoff values, including MHC class IIα, CD11b, CD11c, and CD86, was 87.9, 86.4, 86.4, and 84.8%, respectively.
  
  
  CONCLUSIONS
  Our results suggest that quantitative analysis of mRNA expression of the selected SAs could be an adjunctive diagnostic technique with high diagnostic accuracy for HS in dogs. Substantial investigation is required for exclusion of diseases with similar cell types of origin to lymphoma.
authors.affiliation: Laboratory of Veterinary Surgery, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sappro, Hokkaido, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yamazaki
authors.forename: S
authors.initials: S
authors.lastname: Takagi
authors.forename: N
authors.initials: N
authors.lastname: Oh
authors.forename: Y
authors.initials: Y
authors.lastname: Hoshino
authors.forename: K
authors.initials: K
authors.lastname: Hosoya
authors.forename: M
authors.initials: M
authors.lastname: Okumura
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000235:genetics / Q000276:immunology*; D001706:Biopsy / Q000662:veterinary; D004283:Dog Diseases / Q000235:genetics / Q000276:immunology*; D004285:Dogs; D005260:Female; D054747:Histiocytic Sarcoma / Q000235:genetics / Q000276:immunology*; D006651:Histocytochemistry / Q000662:veterinary; D008297:Male; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D012189:Retrospective Studies; D012680:Sensitivity and Specificity
chemical_list: D000954:Antigens, Surface; D012333:RNA, Messenger
keywords: CD11b; CD11c; CD86; Cancer; Diagnosis; MHC class IIα