title: Paucity of Plasmodium vivax mature schizonts in peripheral blood is associated with their increased cytoadhesive potential.
abstract: There is now a growing body of evidence that challenges the current view that Plasmodium vivax-infected erythrocyte (Pv-iE) are unable to sequester. Here we used ex vivo adhesion assays with Pv-iE before and after maturation to demonstrate a higher binding potential of schizonts compared to other asexual stages. These experimental results are correlated with our observations in a panel of 50 vivax malaria patients where schizonts were completely absent in 27 isolates, and few schizonts were observed in the remaining patients. These observations prompt a paradigm shift in P. vivax biology and open avenues to investigate the role of Pv-iE sequestration.
authors.affiliation: Departamento de Genética, Evolução e Bioagentes, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil.
authors.forename: Stefanie C P
authors.initials: SC
authors.lastname: Lopes
authors.forename: Letusa
authors.initials: L
authors.lastname: Albrecht
authors.forename: Bruna O
authors.initials: BO
authors.lastname: Carvalho
authors.forename: André M
authors.initials: AM
authors.lastname: Siqueira
authors.forename: Richard
authors.initials: R
authors.lastname: Thomson-Luque
authors.forename: Paulo A
authors.initials: PA
authors.lastname: Nogueira
authors.forename: Carmen
authors.initials: C
authors.lastname: Fernandez-Becerra
authors.forename: Hernando A
authors.initials: HA
authors.lastname: Del Portillo
authors.forename: Bruce M
authors.initials: BM
authors.lastname: Russell
authors.forename: Laurent
authors.initials: L
authors.lastname: Rénia
authors.forename: Marcus V G
authors.initials: MV
authors.lastname: Lacerda
authors.forename: Fabio T M
authors.initials: FT
authors.lastname: Costa
mesh_terms: D000962:Antimalarials / Q000627:therapeutic use; D002448:Cell Adhesion / Q000502:physiology*; D002738:Chloroquine / Q000627:therapeutic use; D004912:Erythrocytes / Q000469:parasitology*; D006801:Humans; D016780:Malaria, Vivax / Q000097:blood* / Q000188:drug therapy / Q000469:parasitology*; D018512:Parasitemia / Q000097:blood / Q000469:parasitology; D010966:Plasmodium vivax / Q000254:growth & development / Q000502:physiology*; D011319:Primaquine / Q000627:therapeutic use; D052939:Schizonts / Q000502:physiology; D018709:Statistics, Nonparametric
chemical_list: D000962:Antimalarials; D002738:Chloroquine; D011319:Primaquine
keywords: Plasmodium vivax; cytoadhesion; disproportionality; malaria; schizonts; sequestration