title: Formation of hydrogen peroxide by isolated cell walls from horseradish (Armoracia lapathifolia Gilib.).
abstract: Isolated cell-wall suspensions from horseradish in the presence of 5×10(-4) M MnCl2 catalyze the production of hydrogen peroxide at the expense of either NADPH or NADH. This reaction is inhibited by scavengers of the superoxide free radical ion such as ascorbate or dihydroxyphenols or by superoxide dismutase, and stimulated by monophenols such as p-coumaric acid. On comparison with isolated (commercial) horseradish peroxidase it becomes evident that (a) cell-wall-bound peroxidase(s) is (are) responsible for the production of hydrogenperoxide, involving the superoxide free radical ion as an intermediate of the complex reaction chain.
authors.affiliation: Abteilung Biologie, Lehrstuhl Biochemie der Pflanzen, Ruhr Universität Bochum, P.O. Box 2148, D-4630, Bochum, Federal Republic of Germany.
authors.forename: E F
authors.initials: EF
authors.lastname: Elstner
authors.forename: A
authors.initials: A
authors.lastname: Heupel