title: Cell penetration to nanofibrous scaffolds: Forcespinning®, an alternative approach for fabricating 3D nanofibers.
abstract: Cell infiltration is a critical parameter for the successful development of 3D matrices for tissue engineering. Application of electrospun nanofibers in tissue engineering has recently attracted much attention. Notwithstanding several of their advantages, small pore size and small thickness of the electrospun layer limit their application for development of 3D scaffolds. Several methods for the pore size and/or electrospun layer thickness increase have been recently developed. Nevertheless, tissue engineering still needs emerging of either novel nanofiber-enriched composites or new techniques for 3D nanofiber fabrication. Forcespinning(®) seems to be a promising alternative. The potential of the Forcespinning(®) method is illustrated in preliminary experiment with mesenchymal stem cells.
authors.affiliation: Laboratory of Tissue Engineering; Institute of Experimental Medicine; Academy of Science of the Czech Republic; Prague, Czech Republic; University Center for Energy Efficient Buildings (UCEEB); The Czech Technical University in Prague; Kladno, Czech Republic.
authors.forename: Michala
authors.initials: M
authors.lastname: Rampichová
authors.affiliation: Laboratory of Tissue Engineering; Institute of Experimental Medicine; Academy of Science of the Czech Republic; Prague, Czech Republic; University Center for Energy Efficient Buildings (UCEEB); The Czech Technical University in Prague; Kladno, Czech Republic.
authors.forename: Matej
authors.initials: M
authors.lastname: Buzgo
authors.affiliation: Technical University of Liberec; Faculty of Textile Engineering; Department of Nonwoven Textiles; Liberec, Czech Republic; Technical University of Liberec; Institute for Nanomaterials; Advanced technologies and Innovation; Liberec, Czech Republic.
authors.forename: Jiří
authors.initials: J
authors.lastname: Chvojka
authors.affiliation: Laboratory of Tissue Engineering; Institute of Experimental Medicine; Academy of Science of the Czech Republic; Prague, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Prosecká
authors.affiliation: Laboratory of Molecular Structure Characterization; Institute of Microbiology; Academy of Sciences of the Czech Republic; Prague, Czech Republic.
authors.forename: Olga
authors.initials: O
authors.lastname: Kofroňová
authors.affiliation: Laboratory of Tissue Engineering; Institute of Experimental Medicine; Academy of Science of the Czech Republic; Prague, Czech Republic; University Center for Energy Efficient Buildings (UCEEB); The Czech Technical University in Prague; Kladno, Czech Republic; Institute of Biophysics; 2nd Faculty of Medicine; Charles University in Prague; Prague, Czech Republic; Czech Technical University in Prague; Faculty of Biomedical Engineering; Kladno, Czech Republic.
authors.forename: Evžen
authors.initials: E
authors.lastname: Amler
keywords: Forcespinning®; cell penetration; electrospinning; fibrous scaffold; mesenchymal stem cells