title: Characterization and Expression Analysis of a Retinoblastoma-Related Gene from Chinese WildVitis pseudoreticulata.
abstract: Retinoblastoma-related (RBR) genes, a conserved gene family in higher eukaryotes, play important roles in cell differentiation, development, and mammalian cell death; however, little is known of their function in plants. In this study, a RBR gene was isolated from the Chinese wild grape,Vitis pseudoreticulataW. T. Wang clone "Baihe-35-1", and designated asVpRBR. The cDNA sequence ofVpRBRwas 3,030 bp and contained an open reading frame of 3,024 bp. Conceptual translation of this gene indicated a composition of 1,007 amino acids with a predicted molecular mass of 117.3 kDa. The predicted protein showed a retinoblastoma-associated protein domain A from amino acid residues 416 to 579, and domain B from residues 726 to 855. The result of expression analysis indicated thatVpRBRwas expressed in tissues, leaves, stem, tendrils, flower, and grape skin at different expression levels. Further quantitative reverse transcription-PCR (qRT-PCR) data indicated thatVpRBRlevels were higher inErysiphe necator-treated"Baihe-35-1" and "Baihe-13-1", two resistant clones of Chinese wildV. pseudoreticulata, than inE. necator-treated"Hunan-1", a susceptible clone ofV. pseudoreticulata. Furthermore, the expression ofVpRBRin response to salicylic acid (SA), methyl jasmonate (MeJA), and ethylene (Eth) in grape leaves was also investigated. Taken together, these data indicate thatVpRBRmay contribute to some aspect of powdery mildew resistance in grape.
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Wen
authors.forename: Min
authors.initials: M
authors.lastname: Gao
authors.forename: Chen
authors.initials: C
authors.lastname: Jiao
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.forename: Monika
authors.initials: M
authors.lastname: Walter
authors.forename: Weirong
authors.initials: W
authors.lastname: Xu
authors.forename: Carole
authors.initials: C
authors.lastname: Bassett
authors.forename: Xiping
authors.initials: X
authors.lastname: Wang
keywords: Expression analysis; Powdery mildew; Retinoblastoma-related gene; Vitis pseudoreticulata