title: Streptococcus pneumoniae-induced pneumonia and Citrobacter rodentium-induced gut infection differentially alter vitamin A concentrations in the lung and liver of mice.
abstract: In the developing world, vitamin A (VA) deficiency is endemic in populations that are also at great risk of morbidity and mortality because of pneumococcal pneumonia and enteric infections. To better understand how lung and gastrointestinal pathogens affect VA status, we assessed VA concentrations in serum, lung, and liver during an invasive pneumonia infection induced by Streptococcus pneumoniae serotype 3, and a noninvasive gut infection induced by Citrobacter rodentium, in vitamin A-adequate (VAA) and vitamin A-deficient (VAD) mice. For pneumonia infection, mice were immunized with pneumococcal polysaccharide serotype 3 (PPS3), or not (infected-control), 5 d prior to intranasal inoculation with S. pneumoniae. Two days post-inoculation, immunization was protective against systemic infection regardless of VA status as PPS3 immunization decreased bacteremia compared with infected-control mice (P < 0.05). Retinol concentrations in the lung were higher in infected-control VAA mice (15.7 nmol/g: P < 0.05) compared with PPS3-immunized mice (8.23 nmol/g), but this was not associated with increased lung bacterial burden. VAA mice had reduced severity of C. rodentium-induced gut infection as measured by fecal bacterial shedding compared with VAD mice (P < 0.05). Liver retinol and retinyl ester concentrations in VAA mice decreased at the peak of infection (retinol, 8.1 nmol/g; retinyl esters, 985 nmol/g; P < 0.05, compared with uninfected mice; retinol, 29.5 nmol/g; retinyl esters, 1730 nmol/g), whereas tissue VA concentrations were low in VAD mice during both infections. Colonic mucin gene expression was also depressed at peak infection compared with uninfected mice (P < 0.05). Overall, pneumonia had less effect on VA status than gastrointestinal infection, predominantly owing to reduced hepatic VA storage at the peak of gut infection.
authors.affiliation: Department of Nutritional Sciences.
authors.forename: Katherine H
authors.initials: KH
authors.lastname: Restori
authors.forename: Kaitlin L
authors.initials: KL
authors.lastname: McDaniel
authors.forename: Amanda E
authors.initials: AE
authors.lastname: Wray
authors.forename: Margherita T
authors.initials: MT
authors.lastname: Cantorna
authors.forename: A Catharine
authors.initials: AC
authors.lastname: Ross
mesh_terms: D000818:Animals; D044082:Citrobacter rodentium; D004756:Enterobacteriaceae Infections / Q000503:physiopathology*; D005260:Female; D005767:Gastrointestinal Diseases / Q000382:microbiology / Q000503:physiopathology*; D041981:Gastrointestinal Tract / Q000382:microbiology; D008099:Liver / Q000737:chemistry*; D008168:Lung / Q000737:chemistry*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011018:Pneumonia, Pneumococcal / Q000503:physiopathology*; D013296:Streptococcus pneumoniae; D014611:Vaccination; D014801:Vitamin A / Q000737:chemistry*; D014802:Vitamin A Deficiency / Q000382:microbiology
chemical_list: D014801:Vitamin A