title: Human polyomaviruses 6, 7, 9, 10 and Trichodysplasia spinulosa-associated polyomavirus in HIV-infected men.
abstract: Recently, several novel human polyomaviruses (HPyVs) have been detected. HPyV6, 7, 9 and 10 are not associated with any disease so far. Trichodysplasia spinulosa (TS)-associated polyomavirus (TSPyV) can cause the rare skin disease TS. We have evaluated cutaneous DNA prevalence and viral loads of five HPyVs in HIV-infected men compared to healthy male controls. 449 forehead swabs were analysed by HPyV-specific real-time PCR. HPyV6, HPyV7, TSPyV and HPyV10 were found significantly more frequently on the skin of 210 HIV-infected compared to 239 HIV-negative men (HPyV6, 39.0 vs 27.6 %; HPyV7, 21.0 vs 13.4 %; TSPyV, 3.8 vs 0.8 %; HPyV10, 9.3 vs 3.4 %; P<0.05, respectively). HPyV9 was not detected. Multiple infections were more frequent in HIV-positive men, but HPyV-DNA loads did not differ significantly in both groups. In contrast to HPyV6, 7 and 10, TSPyV and HPyV9 do not seem to be a regular part of the human skin microbiome.
authors.affiliation: Institute of Virology, University of Cologne, 50935 Koeln, Germany.
authors.forename: Ulrike
authors.initials: U
authors.lastname: Wieland
authors.affiliation: Institute of Virology, University of Cologne, 50935 Koeln, Germany.
authors.forename: Steffi
authors.initials: S
authors.lastname: Silling
authors.affiliation: Institute of Medical Statistics, Informatics and Epidemiology, University of Cologne, 50937 Koeln, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Hellmich
authors.affiliation: Department of Dermatology, Venereology and Allergology, Ruhr University Bochum, 44791 Bochum, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Potthoff
authors.affiliation: Institute of Virology, University of Cologne, 50935 Koeln, Germany.
authors.forename: Herbert
authors.initials: H
authors.lastname: Pfister
authors.affiliation: Department of Dermatology, Venereology and Allergology, Ruhr University Bochum, 44791 Bochum, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Kreuter
mesh_terms: D000328:Adult; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005546:Forehead / Q000821:virology; D015658:HIV Infections / Q000150:complications*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011120:Polyomavirus / Q000145:classification* / Q000302:isolation & purification*; D027601:Polyomavirus Infections / Q000453:epidemiology* / Q000821:virology*; D015995:Prevalence; D012867:Skin / Q000821:virology; D014412:Tumor Virus Infections / Q000453:epidemiology* / Q000821:virology*; D019562:Viral Load
chemical_list: D004279:DNA, Viral