title: In-ovulo embryo culture and seedling development of cotton (Gossypium hirsutum L.).
abstract: A convenient and reliable method for culturing cotton embryos is needed to obtain interspecific hybrids of this genus. C.A. Beasley and I.P. Ting (Amer. J. Bot. 60, 130, 1973) developed a phytohormone-supplemented medium (BTP) upon which the growth of ovules was similar that of in situ ovules. This medium was examined for in-ovulo embryo culture. Although good ovule growth occurred on BTP no embryos developed to maturity. However, when the medium was supplemented with NH 4 (+) , more than 50% of the ovules produced mature embryos, and many of these germinated precociously after 8-10 weeks of culture. After germination seedlings were established on a separate medium designed to give balanced root and shoot growth. Subsequently young plants could be transferred to pots for greenhouse culture.
authors.affiliation: Cotton Quality Laboratories, U.S. Department of Agriculture, Agricultural Research Service, Department of Plant and Soil Science, University of Tennessee, 37916, Knoxville, TN, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Stewart
authors.forename: C L
authors.initials: CL
authors.lastname: Hsu