title: Establishment and growth characteristics of a cell suspension culture of Marchantia polymorpha L. with high chlorophyll content.
abstract: A green-pigmented cell suspension culture of Marchantia polymorpha was established using the medium of Murashige and Skoog with addition of organic acids of the tricarboxylic-acid cycle, vitamins and sugars plus sugar alcohols, exclusion of kinetin, and replacement of sucrose with glucose. In continuous light, the cells grew exponentially for ca. 10 days; in the dark, they grew only to a slight extent. The light-grown cells contained well-developed chloroplasts, and chlorophyll content reached almost twice that of the intact gametophyte.
authors.affiliation: The Institute of Food Chemistry, 1-475 Wakayamadai, Shimamoto-cho, Mishima-gun, 618, Osaka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ohta
authors.forename: K
authors.initials: K
authors.lastname: Katoh
authors.forename: K
authors.initials: K
authors.lastname: Miyake