title: Isolation of a novel amylase and lipase-producing Pseudomonas luteola strain: study of amylase production conditions.
abstract: An amylase and lipase producing bacterium (strain C2) was enriched and isolated from soil regularly contaminated with olive washing wastewater in Sfax, Tunisia. Cell was aerobic, mesophilic, Gram-negative, motile, non-sporulating bacterium, capable of growing optimally at pH 7 and 30°C and tolerated maximally 10% (W/V) NaCl. The predominant fatty acids were found to be C(18:1)ω7c (32.8%), C(16:1)ω7c (27.3%) and C16:0 (23.1%). Phylogenetic analysis of the 16S rRNA gene revealed that this strain belonging to the genus Pseudomonas. Strain C2 was found to be closely related to Pseudomonas luteola with more than 99% of similarity. Amylase optimization extraction was carried out using Box Behnken Design (BBD). Its maximal activity was found when the pH and temperature ranged from 5.5 to 6.5 and from 33 to 37°C, respectively. Under these conditions, amylase activity was found to be about 9.48 U/ml.
authors.forename: Lamia
authors.initials: L
authors.lastname: Khannous
authors.forename: Mouna
authors.initials: M
authors.lastname: Jrad
authors.forename: Mouna
authors.initials: M
authors.lastname: Dammak
authors.forename: Ramzi
authors.initials: R
authors.lastname: Miladi
authors.forename: Nour
authors.initials: N
authors.lastname: Chaaben
authors.forename: Bassem
authors.initials: B
authors.lastname: Khemakhem
authors.forename: Néji
authors.initials: N
authors.lastname: Gharsallah
authors.affiliation: Faculté des Sciences de Sfax, Unité de recherche Toxicologie- Microbiologie Environnementale et Santé, Université de, Sfax, Tunisia. imen.fendri@yahoo.fr.
authors.forename: Imen
authors.initials: I
authors.lastname: Fendri
mesh_terms: D000681:Amylases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002701:Chloramphenicol / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D004917:Erythromycin / Q000494:pharmacology; D007612:Kanamycin / Q000494:pharmacology; D008049:Lipase / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D058889:Molecular Typing; D010406:Penicillins / Q000494:pharmacology; D010802:Phylogeny; D011549:Pseudomonas / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013752:Tetracycline / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D010406:Penicillins; D012336:RNA, Ribosomal, 16S; D007612:Kanamycin; D004917:Erythromycin; D002701:Chloramphenicol; D008049:Lipase; D000681:Amylases; D013752:Tetracycline