title: Comparative study between univariate spectrophotometry and multivariate calibration as analytical tools for quantitation of Benazepril alone and in combination with Amlodipine.
abstract: Four simple, accurate, reproducible, and selective methods have been developed and subsequently validated for the determination of Benazepril (BENZ) alone and in combination with Amlodipine (AML) in pharmaceutical dosage form. The first method is pH induced difference spectrophotometry, where BENZ can be measured in presence of AML as it showed maximum absorption at 237nm and 241nm in 0.1N HCl and 0.1N NaOH, respectively, while AML has no wavelength shift in both solvents. The second method is the new Extended Ratio Subtraction Method (EXRSM) coupled to Ratio Subtraction Method (RSM) for determination of both drugs in commercial dosage form. The third and fourth methods are multivariate calibration which include Principal Component Regression (PCR) and Partial Least Squares (PLSs). A detailed validation of the methods was performed following the ICH guidelines and the standard curves were found to be linear in the range of 2-30μg/mL for BENZ in difference and extended ratio subtraction spectrophotometric method, and 5-30 for AML in EXRSM method, with well accepted mean correlation coefficient for each analyte. The intra-day and inter-day precision and accuracy results were well within the acceptable limits.
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
authors.forename: M
authors.initials: M
authors.lastname: Farouk
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
authors.forename: Omar Abd
authors.initials: OA
authors.lastname: Elaziz
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, Cairo, Egypt.
authors.forename: Shereen M
authors.initials: SM
authors.lastname: Tawakkol
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ahram Canadian University, 6th October, Egypt. Electronic address: hemmdan@yahoo.com.
authors.forename: A
authors.initials: A
authors.lastname: Hemdan
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
authors.forename: Mostafa A
authors.initials: MA
authors.lastname: Shehata
mesh_terms: D017311:Amlodipine / Q000032:analysis*; D000959:Antihypertensive Agents / Q000032:analysis*; D001552:Benzazepines / Q000032:analysis*; D002138:Calibration; D016018:Least-Squares Analysis; D015999:Multivariate Analysis; D004364:Pharmaceutical Preparations / Q000737:chemistry; D025341:Principal Component Analysis; D013053:Spectrophotometry / Q000379:methods
chemical_list: D000959:Antihypertensive Agents; D001552:Benzazepines; D004364:Pharmaceutical Preparations; D017311:Amlodipine; C044946:benazepril
keywords: Amlodipine; Benazepril; Difference spectrophotometry; Extended Ratio Subtraction Method; PCR; PLSs