title: Crystallization and preliminary X-ray analysis of a complex of the FOXO1 and Ets1 DNA-binding domains and DNA.
abstract: The Ets1 transcription factor is a member of the Ets protein family, a group of evolutionarily related DNA-binding transcriptional factors. Ets proteins activate or repress the expression of genes that are involved in various biological processes, including cellular proliferation, differentiation, development, transformation and apoptosis. FOXO1 is a member of the forkhead-box proteins (FOX proteins), which comprise a large family of functionally diverse transcription factors involved in cellular proliferation, transformation and differentiation. The FOXO subgroup of FOX proteins regulates the transcription of genes that control metabolism, cell survival, cellular proliferation, DNA damage responses, stress resistance and longevity. The DNA-binding domains (DBDs) of Ets1 and FOXO1 were crystallized in complex with DNA containing a composite sequence for a noncanonical forkhead binding site (AATAACA) and an ETS site (GGAA), FOX:ETS, by the sitting-drop vapor-diffusion method. The FOX:ETS motif has been shown to be a conserved cis-acting element in several endothelial cell-specific genes, including Vegfr2, Tie2, Mef2c and ve-cadherin. Crystals were grown at 291 K using 30% polyethylene glycol 400, 50 mM Tris pH 8.5, 100 mM KCl, 10 mM MgCl2 as the reservoir solution. The crystals belonged to space group C222(1), with unit-cell parameters a = 68.7, b = 104.9, c = 136.3 Å. Diffraction data were collected to a resolution of 2.2 Å.
authors.affiliation: Division of Molecular and Vascular Medicine and Center for Vascular Biology Research, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA.
authors.forename: Wing W
authors.initials: WW
authors.lastname: Choy
authors.affiliation: Division of Molecular and Vascular Medicine and Center for Vascular Biology Research, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA.
authors.forename: Drishadwatti
authors.initials: D
authors.lastname: Datta
authors.affiliation: Division of Molecular and Vascular Medicine and Center for Vascular Biology Research, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA.
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Geiger
authors.affiliation: Division of Experimental Medicine, Department of Medicine, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Birrane
authors.affiliation: Division of Molecular and Vascular Medicine and Center for Vascular Biology Research, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA.
authors.forename: Marianne A
authors.initials: MA
authors.lastname: Grant
mesh_terms: D001483:Base Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D050784:Proto-Oncogene Protein c-ets-1 / Q000737:chemistry* / Q000378:metabolism*
chemical_list: C494041:ETS1 protein, human; C085126:FOXO1 protein, human; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; D050784:Proto-Oncogene Protein c-ets-1; D004247:DNA
keywords: Ets1 transcription factor; FOXO1