title: Metabolism of myo-Inositol and growth in various sugars of suspension-cultured tobacco cells.
abstract: Tobacco (Nicotiana tabacum L.) cells were cultured in a liquid medium which contained sucrose as a source of carbon and energy. Various cell-wall constituents and wall precursors (L-arabinose, D-xylose, D-galactose, D-mannose, D-glucuronate, myo-inositol) were added to cells growing in this medium to by-pass possible rate-limiting steps in the relevant metabolic pathways. None of these compounds stimulated growth as measured by increase in fresh weight; myo-inositol did cause a slight increase and L-arabinose a decrease in dry weight accumulation compared to controls grown on sucrose only. Although myo-inositol was not needed for rapid growth, tracer level amounts of [2-(3)H]myo-inositol were rapidly absorbed and metabolized. Label was incorporated into the uronide and pentose residues of cell walls and exocellular polysaccharide.
authors.affiliation: Department of Botany, University of Illinois, 61801, Urbana, IL, USA.
authors.forename: S
authors.initials: S
authors.lastname: Harran
authors.forename: D B
authors.initials: DB
authors.lastname: Dickinson