title: Maintenance of arytenoid abduction following carbon dioxide laser debridement of the articular cartilage and joint capsule of the cricoarytenoid joint combined with prosthetic laryngoplasty in horses: an in vivo and in vitro study.
abstract: The objective was to evaluate CO2 laser debridement of the cricoarytenoid joint (CAJ) combined with prosthetic laryngoplasty to prevent post-operative loss of arytenoid abduction in seven horses. Horses were assigned to either laser debridement of the left CAJ and laryngoplasty (laser treated, n=5) or control laryngoplasty (sham, n=2), and were evaluated with endoscopic examinations and measurement of right to left angle quotients (RLQ) to assess maintenance of arytenoid abduction. The animals were euthanased at intervals after surgery and larynges were harvested for post-mortem testing, including determination of translaryngeal flow, pressure, impedance and RLQ. Measurements were obtained under increasing vacuum-generated negative pressure with laryngoplasty sutures intact and with the knot/crimp of the laryngoplasty sutures removed. Following post-mortem testing the cricoarytenoid joints were examined histologically. Post-operative endoscopic examinations revealed no significant differences between RLQ measurements calculated for day 1 following surgery to the termination date of the study for the seven horses. Post-mortem RLQ at airflows of 10 and 60 L/s was significantly higher in sham than in laser treated horses both before and after knot/crimp removal. Translaryngeal impedance at 10 and 60 L/s was not statistically different between groups. Histopathology revealed necrosis and loss of articular cartilage in the laser treated horses. The lymphoid cell infiltration subsided but joint capsule and periarticular fibrosis increased over the course of the study. Post-operative loss of arytenoid abduction after laryngoplasty can be minimized with CO2 laser debridement of the CAJ joint.
authors.affiliation: Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Purdue University, 625 Harrison St., West Lafayette, IN 47907, USA. Electronic address: hawkinsj@purdue.edu.
authors.forename: J F
authors.initials: JF
authors.lastname: Hawkins
authors.affiliation: Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Purdue University, 625 Harrison St., West Lafayette, IN 47907, USA.
authors.forename: L
authors.initials: L
authors.lastname: Couetil
authors.affiliation: Department of Comparative Pathobiology, Purdue University, 625 Harrison St., West Lafayette, IN 47907, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Miller
mesh_terms: D000818:Animals; D001193:Arytenoid Cartilage / Q000601:surgery; D002358:Cartilage, Articular / Q000601:surgery; D003413:Cricoid Cartilage / Q000601:surgery; D003646:Debridement / Q000379:methods / Q000662:veterinary*; D006734:Horse Diseases / Q000601:surgery*; D006736:Horses; D017746:Joint Capsule; D007821:Laryngeal Muscles / Q000294:innervation / Q000503:physiopathology* / Q000601:surgery; D058753:Laryngoplasty / Q000662:veterinary*; D010523:Peripheral Nervous System Diseases / Q000601:surgery / Q000662:veterinary*; D012009:Recurrent Laryngeal Nerve
keywords: Cricoarytenoid joint; Equine; Laryngeal hemiplegia; Laser; Respiratory