title: Polyamine metabolism and lipoxygenase activity during Fusarium oxysporum f. sp. ricini -Castor interaction.
abstract: Polyamine oxidase and lipoxygenase enzymes are key players for hyper sensitive reaction (HR) during incompatible interaction of host-pathogen. Thus, the role of lipoxygenase and polyamines was studied in the wilt pathogen infected and non infected tissues of resistant and susceptible genotypes of castor at 0 days after infection (DAI), 5 DAI and 10 DAI (30 days after sowing). The lipoxygenase (LOX) and polyamine oxidase (PAO) activities were higher in the incompatible interaction at all the stages of analysis. The constitutive level of malondyaldehyde (MDA) content, a product of lipid peroxidation was higher in susceptible genotypes (VP-1 and VI-9), while induced level was higher in resistant genotypes (48-1 and SKP-84) at 5 DAI and 10 DAI . Polyamine profiling using HPTLC showed higher spermidine and spermine content in resistant genotypes at 10 DAI. Furthermore, spermidine was detected only in the roots of resistant genotypes at 10 DAI. These results suggest the role of high titers of polyamines, LOX and PAO in disease resistance possibly through HR induction.
authors.affiliation: Department of Plant Molecular Biology and Biotechnology, Navsari Agricultural University, Navsari, 396 450 India.
authors.forename: Somnath D
authors.initials: SD
authors.lastname: Mhaske
authors.affiliation: Department of Plant Molecular Biology and Biotechnology, Navsari Agricultural University, Navsari, 396 450 India ; Directorate of Groundnut Research, Indian Council of Agricultural Research, Post Box # 5, Junagdh, 362 001 India.
authors.forename: Mahesh Kumar
authors.initials: MK
authors.lastname: Mahatma
authors.affiliation: Department of Plant Molecular Biology and Biotechnology, Navsari Agricultural University, Navsari, 396 450 India.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Jha
authors.affiliation: Department of Plant Pathology, ASPEE College of Horticulture & Forestry, Navsari Agricultural University, Navsari, 396 450 India.
authors.forename: Pushpendra
authors.initials: P
authors.lastname: Singh
authors.affiliation: Department of Plant Molecular Biology and Biotechnology, Navsari Agricultural University, Navsari, 396 450 India.
authors.forename: Taslim
authors.initials: T
authors.lastname: Ahmad
keywords: Castor; HPTLC; Lipoxygenase; MDA; Polyamines; Wilt