title: On one leg: trehalose monoesters activate macrophages in a Mincle-dependant manner.
abstract: The C22 and C26 trehalose monoesters, each containing a single acyl chain, were synthesised in good overall yields and found to activate macrophages in a Mincle-dependent manner. The activities of the monoesters paralleled those of their diester counterparts, and both mono- and diesters could activate the immune response in the absence of priming. This is the first time that trehalose monoesters have been found to activate macrophages, and these studies thus provide an important framework for the rational design of other Mincle agonists.
authors.affiliation: School of Chemical and Physical Sciences, Victoria University of Wellington, P. O. Box 600, 6140 Wellington (New Zealand); Malaghan Institute of Medical Research, P. O. Box 7060, 6242 Wellington (New Zealand). bridget.stocker@vuw.ac.nz.
authors.forename: Bridget L
authors.initials: BL
authors.lastname: Stocker
authors.forename: Ashna A
authors.initials: AA
authors.lastname: Khan
authors.forename: Stephanie H
authors.initials: SH
authors.lastname: Chee
authors.forename: Faustin
authors.initials: F
authors.lastname: Kamena
authors.forename: Mattie S M
authors.initials: MS
authors.lastname: Timmer
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D004952:Esters / Q000494:pharmacology; D006017:Glycolipids / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D015850:Interleukin-6 / Q000378:metabolism; D037181:Lectins, C-Type / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D008565:Membrane Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009569:Nitric Oxide / Q000378:metabolism; D051195:Toll-Like Receptor 2 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D014199:Trehalose / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology
chemical_list: C000591902:6-O-docosanoyl-alpha,alpha'-trehalose; C000591903:6-O-hexacosanoyl-alpha,alpha'-trehalose; C113583:Clecsf8 protein, mouse; D004952:Esters; D006017:Glycolipids; D015850:Interleukin-6; D037181:Lectins, C-Type; D008565:Membrane Proteins; C493486:Tlr2 protein, mouse; C493487:Tlr4 protein, mouse; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D009569:Nitric Oxide; D014199:Trehalose
keywords: Mincle; adjuvants; glycolipids; immunology; macrophages