title: A rapid differentiation method for enteroinvasive Escherichia coli.
abstract: Enteroinvasive Escherichia coli (EIEC) comprise 21 major serotypes defined by the presence of O and H antigens, and diagnosis depends on determining its invasive potential. Using HEp-2 cells infected with an EIEC strain, we developed a simple growth-dependent assay that differentiated EIEC strain from non-invasive strains 6 h after infection.
authors.affiliation: National Institute of Animal Health, NARO Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Swarmistha Devi
authors.initials: SD
authors.lastname: Aribam
authors.affiliation: National Institute of Animal Health, NARO Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Jiro
authors.initials: J
authors.lastname: Hirota
authors.affiliation: National Institute of Animal Health, NARO Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kusumoto
authors.affiliation: National Institute of Animal Health, NARO Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Harada
authors.affiliation: National Institute of Animal Health, NARO Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Shiraiwa
authors.affiliation: National Institute of Animal Health, NARO Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Yohsuke
authors.initials: Y
authors.lastname: Ogawa
authors.affiliation: National Institute of Animal Health, NARO Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Shimoji
authors.affiliation: National Institute of Animal Health, NARO Tsukuba, Ibaraki 305-0856, Japan. Electronic address: egumaro@affrc.go.jp.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Eguchi
mesh_terms: D001681:Biological Assay / Q000379:methods*; D002460:Cell Line; D004926:Escherichia coli / Q000254:growth & development*; D004927:Escherichia coli Infections / Q000175:diagnosis*; D006801:Humans
keywords: Enteroinvasive Escherichia coli; Gentamicin invasion assay; Rapid detection