title: Motif-directed redesign of enzyme specificity.
abstract: Computational protein design relies on several approximations, including the use of fixed backbones and rotamers, to reduce protein design to a computationally tractable problem. However, allowing backbone and off-rotamer flexibility leads to more accurate designs and greater conformational diversity. Exhaustive sampling of this additional conformational space is challenging, and often impossible. Here, we report a computational method that utilizes a preselected library of native interactions to direct backbone flexibility to accommodate placement of these functional contacts. Using these native interaction modules, termed motifs, improves the likelihood that the interaction can be realized, provided that suitable backbone perturbations can be identified. Furthermore, it allows a directed search of the conformational space, reducing the sampling needed to find low energy conformations. We implemented the motif-based design algorithm in Rosetta, and tested the efficacy of this method by redesigning the substrate specificity of methionine aminopeptidase. In summary, native enzymes have evolved to catalyze a wide range of chemical reactions with extraordinary specificity. Computational enzyme design seeks to generate novel chemical activities by altering the target substrates of these existing enzymes. We have implemented a novel approach to redesign the specificity of an enzyme and demonstrated its effectiveness on a model system.
authors.affiliation: Program in Computational and Systems Biology, Washington University in St. Louis, St. Louis, Missouri, 63110.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Borgo
authors.forename: James J
authors.initials: JJ
authors.lastname: Havranek
mesh_terms: D000465:Algorithms; D020816:Amino Acid Motifs; D003198:Computer Simulation; D016384:Consensus Sequence; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism*; D008956:Models, Chemical; D008958:Models, Molecular; D019151:Peptide Library; D011485:Protein Binding; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D013379:Substrate Specificity
chemical_list: D004798:Enzymes; D019151:Peptide Library
keywords: computational protein design; enzyme design; molecular specificity; mutagenesis