title: Hemoadsorption of high-mobility group box chromosomal protein 1 using a column for large animals.
abstract: BACKGROUND
  High-mobility group box chromosomal protein 1 (HMGB1) has recently been identified as an important mediator of various kinds of acute and chronic inflammation. A method for efficiently removing HMGB1 from the systemic circulation could be a promising therapy for HMGB1-mediated inflammatory diseases.
  
  
  METHODS
  In this study, we produced a new adsorbent material by chemically treating polystyrene fiber. We first determined whether the adsorbent material efficiently adsorbed HMGB1 in vitro using a bovine HMGB1 solution and a plasma sample from a swine model of acute liver failure. We then constructed a column by embedding fabric sheets of the newly developed fibers into a cartridge and tested the ability of the column to reduce plasma HMGB1 levels during a 4-hour extracorporeal hemoperfusion in a swine model of acute liver failure.
  
  
  RESULTS
  The in vitro adsorption test of the new fiber showed high performance for HMGB1 adsorption (96% adsorption in the bovine HMGB1 solution and 94% in the acute liver failure swine plasma, 2 h incubation at 37°C; p < 0.05 vs. incubation with no adsorbent). In the in vivo study, the ratio of the HMGB1 concentration at the outlet versus the inlet of the column was significantly lower in swine hemoperfused with the newly developed column (53 and 61% at the beginning and end of perfusion, respectively) than in those animals hemoperfused with the control column (94 and 93% at the beginning and end of perfusion, respectively; p < 0.05). Moreover, the normalized plasma level of HMGB1 was significantly lower during perfusion with the new column than with the control column (p < 0.05 at 1, 2, and 3 h after initiation of perfusion).
  
  
  CONCLUSIONS
  These data suggest that the newly developed column has the potential to effectively adsorb HMGB1 during hemoperfusion in swine.
authors.affiliation: Department of Surgery, Keio University School of Medicine, Tokyo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Nishiyama
authors.forename: M
authors.initials: M
authors.lastname: Shinoda
authors.forename: M
authors.initials: M
authors.lastname: Tanabe
authors.forename: G
authors.initials: G
authors.lastname: Oshima
authors.forename: K
authors.initials: K
authors.lastname: Takano
authors.forename: T
authors.initials: T
authors.lastname: Miyasho
authors.forename: Y
authors.initials: Y
authors.lastname: Fuchimoto
authors.forename: S
authors.initials: S
authors.lastname: Yamada
authors.forename: T
authors.initials: T
authors.lastname: Inoue
authors.forename: K
authors.initials: K
authors.lastname: Shimada
authors.forename: K
authors.initials: K
authors.lastname: Suda
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
authors.forename: T
authors.initials: T
authors.lastname: Hayashida
authors.forename: H
authors.initials: H
authors.lastname: Yagi
authors.forename: M
authors.initials: M
authors.lastname: Kitago
authors.forename: H
authors.initials: H
authors.lastname: Obara
authors.forename: O
authors.initials: O
authors.lastname: Itano
authors.forename: H
authors.initials: H
authors.lastname: Takeuchi
authors.forename: S
authors.initials: S
authors.lastname: Kawachi
authors.forename: I
authors.initials: I
authors.lastname: Maruyama
authors.forename: Y
authors.initials: Y
authors.lastname: Kitagawa
mesh_terms: D000327:Adsorption; D000818:Animals; D024243:HMGB1 Protein / Q000097:blood* / Q000302:isolation & purification; D006464:Hemoperfusion / Q000379:methods*; D017114:Liver Failure, Acute / Q000097:blood / Q000628:therapy; D008297:Male; D013552:Swine
chemical_list: D024243:HMGB1 Protein