title: Performance evaluation of the automated morphological analysis of erythrocytes by CellaVision DM96.
abstract: BACKGROUND
  Automated digital morphology systems are utilized for blood cell morphological examination. The aim of this study is to evaluate the accuracy and efficacy of RBC morphological anomaly screening using the CellaVision DM96 (DM96) automated image analysis system.
  
  
  METHODS
  The automated analysis of RBC shape, size, and chromasia abnormalities was conducted on the DM96 using 478 blood samples. A manual microscopic review was independently performed.
  
  
  RESULTS
  The DM96 preclassified samples as poikilocytosis-positive for 98% of cases with schistocytosis or echinocytosis, 97% of elliptocytosis, and 92% or 65% of cases that were positive for teardrop cells or for target cells, respectively. The accuracy of the DM96 in the detection of RBC size and chromasia abnormalities of iron deficiency anemia cases was higher than direct microscopic observation.
  
  
  CONCLUSIONS
  Automated morphological analysis with the DM96 has potential utility in the morphological screening of RBC anomalies that are associated with disease.
authors.affiliation: Division of Clinical Laboratory, Juntendo University Hospital, Bunkyo-ku, Tokyo, Japan.
authors.forename: Imiko
authors.initials: I
authors.lastname: Maenou
authors.affiliation: Department of Clinical Laboratory Medicine, Juntendo University School of Medicine, Bunkyo-ku, Tokyo, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Tabe
authors.affiliation: CellaVision AB, Ideon Science Park, Lund, Sweden.
authors.forename: Hans-Inge
authors.initials: HI
authors.lastname: Bengtsson
authors.affiliation: Division of Clinical Laboratory, Juntendo University Hospital, Bunkyo-ku, Tokyo, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ishii
authors.affiliation: Department of Clinical Laboratory Medicine, Juntendo University School of Medicine, Bunkyo-ku, Tokyo, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Miyake
authors.affiliation: Department of Clinical Laboratory Medicine, Juntendo University School of Medicine, Bunkyo-ku, Tokyo, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Horiuchi
authors.affiliation: Department of Clinical Laboratory Medicine, Juntendo University School of Medicine, Bunkyo-ku, Tokyo, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Idei
authors.affiliation: Department of Clinical Laboratory Medicine, Juntendo University School of Medicine, Bunkyo-ku, Tokyo, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Horii
authors.affiliation: Division of Clinical Laboratory, Juntendo Tokyo Koto Geriatric Medical Center, Koto-ku, Tokyo, Japan.
authors.forename: Naotake
authors.initials: N
authors.lastname: Satoh
authors.affiliation: Department of Clinical Laboratory Medicine, Juntendo University School of Medicine, Bunkyo-ku, Tokyo, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Miida
authors.affiliation: Division of Clinical Laboratory, Juntendo University Hospital, Bunkyo-ku, Tokyo, Japan.
authors.forename: Akimichi
authors.initials: A
authors.lastname: Ohsaka
mesh_terms: D001331:Automation; D002469:Cell Separation / Q000295:instrumentation*; D004912:Erythrocytes / Q000166:cytology*; D006801:Humans