title: Differential cleavage of IRES trans-acting factors (ITAFs) in cells infected by human rhinovirus.
abstract: Human rhinovirus (HRV) is a major causative agent of the common cold, and thus has several important health implications. As a member of the picornavirus family, HRV has a small genomic RNA that utilizes several host cell proteins for RNA replication. Host proteins poly(rC) binding protein 2 (PCBP2) and polypyrimidine tract binding protein (PTB) are cleaved by a viral proteinase during the course of infection by the related picornavirus, poliovirus. The cleavage of PCBP2 and PTB inhibits poliovirus translation and has been proposed to mediate a switch in poliovirus template usage from translation to RNA replication. HRV RNA replication also requires a switch in template usage from translation to RNA replication; however, the mechanism is not yet known. We demonstrate that PCBP2 and PTB are differentially cleaved during HRV infection in different cell lines, suggesting that HRV utilizes a mechanism distinct from PCBP2 or PTB cleavage to mediate a switch in template usage.
authors.affiliation: Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, CA 92697, USA.
authors.forename: Amanda J
authors.initials: AJ
authors.lastname: Chase
authors.affiliation: Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, CA 92697, USA. Electronic address: blsemler@uci.edu.
authors.forename: Bert L
authors.initials: BL
authors.lastname: Semler
mesh_terms: D015967:Gene Expression Regulation, Viral; D006367:HeLa Cells; D006801:Humans; D010447:Peptide Hydrolases / Q000235:genetics / Q000378:metabolism; D010850:Picornaviridae Infections / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D038941:Polypyrimidine Tract-Binding Protein / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012229:Rhinovirus / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: C501903:PCBP2 protein, human; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D014764:Viral Proteins; D038941:Polypyrimidine Tract-Binding Protein; D010447:Peptide Hydrolases
keywords: Coxsackievirus; Host cell protein cleavage; Human rhinovirus; PCBP2; PTB; Picornavirus; Poliovirus; Viral proteinases