title: Cellular uptake, cytotoxicity, apoptosis and DNA-binding investigations of Ru(II) complexes.
abstract: Three new compounds, [Ru(Hdpa)2PyIP](ClO4)2·2H2O (1) [Ru(Hdpa)2FyIP](ClO4)2·2H2O (2) and [Ru(Hdpa)2IIP](ClO4)2·2H2O (3) have been synthesized and characterized by spectroscopic techniques such as elemental analysis, UV/Vis, FT-IR, (1)H NMR, (13)C NMR and mass spectra. The CT-DNA binding properties of 1-3 have been investigated by absorption, emission spectroscopy and viscosity measurements. Experimental results suggested that they can interact with DNA through intercalative mode with different binding strengths. These were found to promote the cleavage of plasmid DNA. Cell viability results indicated that all compounds showed significant dose dependent cytotoxicity in selected cell lines and 1 shown higher cytotoxicity than cisplatin on HeLa cells. Cellular uptake studies were studied by flow cytometry and confocal microscopy.
authors.affiliation: Department of Chemistry, Osmania University, Hyderabad 500007, Andhra Pradesh, India.
authors.forename: C
authors.initials: C
authors.lastname: Shobha Devi
authors.affiliation: Department of Chemistry, Osmania University, Hyderabad 500007, Andhra Pradesh, India.
authors.forename: Penumaka
authors.initials: P
authors.lastname: Nagababu
authors.affiliation: Department of Biochemistry, Osmania University, Hyderabad 500007, India.
authors.forename: Sumathi
authors.initials: S
authors.lastname: Natarajan
authors.affiliation: Department of Chemistry, Osmania University, Hyderabad 500007, Andhra Pradesh, India.
authors.forename: N
authors.initials: N
authors.lastname: Deepika
authors.affiliation: Department of Chemistry, Osmania University, Hyderabad 500007, Andhra Pradesh, India.
authors.forename: P
authors.initials: P
authors.lastname: Venkat Reddy
authors.affiliation: Department of Biochemistry, Osmania University, Hyderabad 500007, India.
authors.forename: N
authors.initials: N
authors.lastname: Veerababu
authors.affiliation: Department of Biochemistry, Osmania University, Hyderabad 500007, India.
authors.forename: Surya S
authors.initials: SS
authors.lastname: Singh
authors.affiliation: Department of Chemistry, Osmania University, Hyderabad 500007, Andhra Pradesh, India. Electronic address: ssnsirasani@gmail.com.
authors.forename: S
authors.initials: S
authors.lastname: Satyanarayana
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001665:Binding Sites / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000737:chemistry / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D006367:HeLa Cells; D006801:Humans; D008958:Models, Molecular; D008968:Molecular Conformation; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D012413:Rubidium / Q000737:chemistry*; D013329:Structure-Activity Relationship
chemical_list: D000970:Antineoplastic Agents; D009942:Organometallic Compounds; D004247:DNA; D012413:Rubidium
keywords: Apoptosis; Cellular uptake; Cytotoxicity; DNA binding; Fluorescence