title: Screening for PCDD/Fs and dl-PCBs in local and imported food and feed products available across the State of Kuwait and assessment of dietary intake.
abstract: A total of 318 local and imported meat, milk, eggs, fish, and animal feed samples collected in Kuwait were analyzed by cell-based reporter gene assay (Dioxin-Responsive Chemical Activated LUciferase gene eXpression DR-CALUX) for PCDD/Fs and dl-PCBs. The bioanalytical equivalents (BEQs) obtained by DR-CALUX bioassay were compared with the official maximum limits according to the European Commission (EC) regulations. Suspected and randomly chosen negative samples were analyzed by gas chromatography-high resolution mass spectrometry (GC-HRMS). The results showed that among suspected samples, one sample was confirmed to be non-compliant. The positive sample was of imported origin. The correlation coefficient of 0.98 between DR-CALUX and GC-HRMS was found. Moreover, the average daily intakes of PCDD/Fs and dl-PCBs for the Kuwaiti population were estimated. Results obtained in this study were discussed and compared with other published data.
authors.affiliation: Food and Nutrition Program, Environmental and Life Sciences Research Center, Kuwait Institute for Scientific Research, P.O. Box 24885, Safat 13109, Kuwait. Electronic address: ahussain@kisr.edu.kw.
authors.forename: Adnan
authors.initials: A
authors.lastname: Husain
authors.affiliation: Environment Management Program, Environmental and Life Sciences Research Center, Kuwait Institute for Scientific Research, P.O. Box 24885, Safat 13109, Kuwait.
authors.forename: Bondi
authors.initials: B
authors.lastname: Gevao
authors.affiliation: Food and Nutrition Program, Environmental and Life Sciences Research Center, Kuwait Institute for Scientific Research, P.O. Box 24885, Safat 13109, Kuwait.
authors.forename: Basma
authors.initials: B
authors.lastname: Dashti
authors.affiliation: BioDetection Systems BV (BDS), Science Park 406, 1098 XH Amsterdam, The Netherlands.
authors.forename: Abraham
authors.initials: A
authors.lastname: Brouwer
authors.affiliation: BioDetection Systems BV (BDS), Science Park 406, 1098 XH Amsterdam, The Netherlands.
authors.forename: Peter Aleaxnder
authors.initials: PA
authors.lastname: Behnisch
authors.affiliation: Environment Management Program, Environmental and Life Sciences Research Center, Kuwait Institute for Scientific Research, P.O. Box 24885, Safat 13109, Kuwait.
authors.forename: Majed
authors.initials: M
authors.lastname: Al-Wadi
authors.affiliation: Food and Nutrition Program, Environmental and Life Sciences Research Center, Kuwait Institute for Scientific Research, P.O. Box 24885, Safat 13109, Kuwait.
authors.forename: Mohamad
authors.initials: M
authors.lastname: Al-Foudari
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D001572:Benzofurans / Q000032:analysis; D001681:Biological Assay / Q000379:methods; D004435:Eating; D005504:Food Analysis; D008401:Gas Chromatography-Mass Spectrometry; D007730:Kuwait; D011078:Polychlorinated Biphenyls / Q000032:analysis*; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D001572:Benzofurans; D000072317:Polychlorinated Dibenzodioxins; D011078:Polychlorinated Biphenyls
keywords: DR-CALUX bioassay; Feed; Food; PCDD/Fs and dl-PCBs