title: Vallitalea pronyensis sp. nov., isolated from a marine alkaline hydrothermal chimney.
abstract: A novel thermotolerant, anaerobic, Gram-stain-positive, spore-forming bacterium was isolated from a hydrothermal chimney in Prony Bay, New Caledonia. This strain, designated FatNI3(T), grew at 15-55 °C (optimum 30 °C) and at pH 5.8-8.9 (optimum 7.7). It was slightly halophilic, requiring at least 0.5 % NaCl for growth (optimum 2.5-3.0 %), and was able to grow at up to 6 % NaCl. Sulfate, thiosulfate, elemental sulfur, sulfite, nitrate and nitrite were not used as terminal electron acceptors. Growth of strain FatNI3(T) was inhibited in the presence of sulfite (2 mM) or nitrite (2 mM). Strain FatNI3(T) fermented cellobiose, glucose, mannose, maltose, sucrose, galactose, lactose, ribose, fructose, rhamnose, raffinose, xylose, yeast extract, peptone and biotrypticase. The main fermentation products from glucose metabolism were acetate, ethanol, H2 and CO2. The predominant cellular fatty acids were iso-C15 : 0 and anteiso-C15 : 0. The main polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, and unknown glycolipids and phospholipids. The G+C content of the genomic DNA was 36.6 mol%. On the basis of phylogenetic and physiological properties, strain FatNI3(T) ( = DSM 25904 = JCM 18391) belonging to the phylum Firmicutes, class Clostridia, order Clostridiales, is proposed as the type strain of a novel species of the genus Vallitalea, for which the name Vallitalea pronyensis sp. nov. is proposed.
authors.affiliation: Laboratoire d'Ecologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologie, Centre Urbain Nord, BP 676, 1080 Tunis Cedex, Tunisia.|Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM 110, 13288, Marseille, cedex 09, France.
authors.forename: Fatma
authors.initials: F
authors.lastname: Ben Aissa
authors.affiliation: Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM 110, 13288, Marseille, cedex 09, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Postec
authors.affiliation: Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM 110, 13288, Marseille, cedex 09, France.
authors.forename: Gaël
authors.initials: G
authors.lastname: Erauso
authors.affiliation: Centre IRD de Nouméa, 101 Promenade Roger Laroque, BP A5 - 98848 Nouméa cedex, New Caledonia.
authors.forename: Claude
authors.initials: C
authors.lastname: Payri
authors.affiliation: Centre IRD de Nouméa, 101 Promenade Roger Laroque, BP A5 - 98848 Nouméa cedex, New Caledonia.
authors.forename: Bernard
authors.initials: B
authors.lastname: Pelletier
authors.affiliation: Laboratoire d'Ecologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologie, Centre Urbain Nord, BP 676, 1080 Tunis Cedex, Tunisia.
authors.forename: Moktar
authors.initials: M
authors.lastname: Hamdi
authors.affiliation: Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM 110, 13288, Marseille, cedex 09, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Ollivier
authors.affiliation: Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM 110, 13288, Marseille, cedex 09, France.
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Fardeau
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D060086:Hydrothermal Vents / Q000382:microbiology*; D008969:Molecular Sequence Data; D009510:New Caledonia; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S