title: Long term intake of 0.1% ethanol decreases serum adiponectin by suppressing PPARγ expression via p38 MAPK pathway.
abstract: Light alcohol consumption was reported to be negatively associated with insulin resistance and risk of cardiovascular diseases; however, the results were inconsistent. We here investigate whether long term intake of low-concentration ethanol can affect adiponectin levels. Male Wistar rats were exposed to 0.1% ethanol in drinking water for 26weeks. Visceral adipose tissue (VAT) was cultured and treated with ethanol, SB203580, GW9662, or rosiglitazone. Adiponectin in serum and culture supernatant were measured by ELISA, mRNA levels of adiponectin and PPARγ were determined by RT-PCR, and protein expressions of PPARγ, p38 MAPK and phospho-p38 MAPK were determined by Western blot. In vivo, ethanol decreased the mRNA of adiponectin in VAT and serum adiponectin significantly. Decreased PPARγ and increased activation of p38 MAPK were observed in ethanol treated group. In vitro, SB203580 increased the adiponectin and PPARγ levels in normal DMEM cultured VAT and ameliorated ethanol-induced decrease of adiponectin and PPARγ expressions. GW9662 also decreased the adiponectin levels; Both ethanol and GW9662 weakened the rosiglitazone-induced elevation of adiponectin levels in cultured VAT. These data suggest that long term intake of 0.1% ethanol down-regulated adiponectin levels, and the regulation of PPARγ via p38 MAPK pathway plays an important role in the mechanism underneath.
authors.affiliation: Department of Nutrition & Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, PR China; Department of Nursing, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, PR China.
authors.forename: Chong
authors.initials: C
authors.lastname: Tian
authors.affiliation: Department of Nutrition & Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, PR China; Ministry of Education Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Jin
authors.affiliation: Department of Public Health, Wenzhou Medical University, Wenzhou, PR China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Ye
authors.affiliation: Department of Public Health, Wenzhou Medical University, Wenzhou, PR China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Nutrition & Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, PR China; Ministry of Education Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, PR China.
authors.forename: Weiye
authors.initials: W
authors.lastname: Ren
authors.affiliation: Department of Nutrition & Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, PR China; Ministry of Education Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Nutrition & Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, PR China; Ministry of Education Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, PR China.
authors.forename: Jia
authors.initials: J
authors.lastname: Long
authors.affiliation: Department of Nutrition & Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, PR China; Ministry of Education Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, PR China. Electronic address: yingchenjiang@yahoo.com.
authors.forename: Chenjiang
authors.initials: C
authors.lastname: Ying
mesh_terms: D052242:Adiponectin / Q000097:blood* / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D000431:Ethanol / Q000008:administration & dosage*; D050152:Intra-Abdominal Fat / Q000378:metabolism; D008297:Male; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D052242:Adiponectin; D017931:DNA Primers; D047495:PPAR gamma; D012333:RNA, Messenger; D000431:Ethanol; D048051:p38 Mitogen-Activated Protein Kinases
keywords: Adiponectin; Ethanol; Long-term exposure; PPARγ; p38 MAPK