title: Evolution of a cell culture-derived genotype 1a hepatitis C virus (H77S.2) during persistent infection with chronic hepatitis in a chimpanzee.
abstract: Persistent infection is a key feature of hepatitis C virus (HCV). However, chimpanzee infections with cell culture-derived viruses (JFH1 or related chimeric viruses that replicate efficiently in cell culture) have been limited to acute-transient infections with no pathogenicity. Here, we report persistent infection with chronic hepatitis in a chimpanzee challenged with cell culture-derived genotype 1a virus (H77S.2) containing 6 cell culture-adaptive mutations. Following acute-transient infection with a chimeric H77/JFH1 virus (HJ3-5), intravenous (i.v.) challenge with 10(6) FFU H77S.2 virus resulted in immediate seroconversion and, following an unusual 4- to 6-week delay, persistent viremia accompanied by alanine aminotransferase (ALT) elevation, intrahepatic innate immune responses, and diffuse hepatopathy. This first persistent infection with cell culture-produced HCV provided a unique opportunity to assess evolution of cell culture-adapted virus in vivo. Synonymous and nonsynonymous nucleotide substitution rates were greatest during the first 8 weeks of infection. Of 6 cell culture-adaptive mutations in H77S.2, Q1067R (NS3) had reverted to Q1067 and S2204I (NS5A) was replaced by T2204 within 8 weeks of infection. By 62 weeks, 4 of 6 mutations had reverted to the wild-type sequence, and all reverted to the wild-type sequence by 194 weeks. The data suggest H77S.2 virus has greater potential for persistence and pathogenicity than JFH1 and demonstrate both the capacity of a nonfit virus to persist for weeks in the liver in the absence of detectable viremia as well as strong selective pressure against cell culture-adaptive mutations in vivo.
  
  
  OBJECTIVE
  This study shows that mutations promoting the production of infectious genotype 1a HCV in cell culture have the opposite effect and attenuate replication in the liver of the only fully permissive animal species other than humans. It provides the only example to date of persistent infection in a chimpanzee challenged with cell culture-produced virus and provides novel insight into the forces shaping molecular evolution of that virus during 5 years of persistent infection. It demonstrates that a poorly fit virus can replicate for weeks within the liver in the absence of detectable viremia, an observation that expands current concepts of HCV pathogenesis and that is relevant to relapses observed with direct-acting antiviral therapies.
authors.affiliation: Department of Microbiology & Immunology, University of Texas Medical Branch, Galveston, Texas, USA.
authors.forename: MinKyung
authors.initials: M
authors.lastname: Yi
authors.forename: Fengyu
authors.initials: F
authors.lastname: Hu
authors.forename: Michael
authors.initials: M
authors.lastname: Joyce
authors.forename: Vikas
authors.initials: V
authors.lastname: Saxena
authors.forename: Christoph
authors.initials: C
authors.lastname: Welsch
authors.forename: Deborah
authors.initials: D
authors.lastname: Chavez
authors.forename: Bernadette
authors.initials: B
authors.lastname: Guerra
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yamane
authors.forename: Ronald
authors.initials: R
authors.lastname: Veselenak
authors.forename: Rick
authors.initials: R
authors.lastname: Pyles
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Walker
authors.forename: Lorne
authors.initials: L
authors.lastname: Tyrrell
authors.forename: Nigel
authors.initials: N
authors.lastname: Bourne
authors.forename: Robert E
authors.initials: RE
authors.lastname: Lanford
authors.forename: Stanley M
authors.initials: SM
authors.lastname: Lemon
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D004195:Disease Models, Animal; D019143:Evolution, Molecular; D005838:Genotype; D016174:Hepacivirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D019698:Hepatitis C, Chronic / Q000821:virology*; D008099:Liver / Q000473:pathology; D009154:Mutation; D002679:Pan troglodytes; D014766:Viremia; D014776:Virus Cultivation
chemical_list: D000410:Alanine Transaminase