title: Primordial follicle assembly was regulated by Notch signaling pathway in the mice.
abstract: Notch signaling pathway, a highly conserved cell signaling system, exists in most multicellular organisms. The objective of this study was to examine Notch signaling pathway in germ cell cyst breakdown and primordial follicle formation. The receptor and ligand genes of Notch pathway (Notch1, Notch2, Jagged1, Jagged2 and Hes1) were extremely down-regulated after newborn mouse ovaries were cultured then exposed to DAPT or L-685,458 in vitro (P < 0.01). Since DAPT or L-685,548 inhibits Notch signaling pathway, the expression of protein LHX8 and NOBOX was significantly reduced during the formation of the primordial follicles. Down-regulated mRNA expression of specific genes including Lhx8, Figla, Sohlh2 and Nobox, were also observed. The percentages of female germ cells in germ cell cysts and primordial follicles were counted after culture of newborn ovaries for 3 days in vitro. The result showed female germ cells in cysts was remarkably up-regulated while as the oocytes in primordial follicles was significantly down-regulated (P < 0.05). In conclusion, Notch signaling pathway may regulate the formation of primordial follicle in mice.
authors.affiliation: Laboratory of Germ Cell Biology, Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, 266109, China, chenchunlei2659798@126.com.
authors.forename: Chun-Lei
authors.initials: CL
authors.lastname: Chen
authors.forename: Xia-Fei
authors.initials: XF
authors.lastname: Fu
authors.forename: Lin-Qing
authors.initials: LQ
authors.lastname: Wang
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Wang
authors.forename: Hua-Gang
authors.initials: HG
authors.lastname: Ma
authors.forename: Shun-Feng
authors.initials: SF
authors.lastname: Cheng
authors.forename: Zhu-Mei
authors.initials: ZM
authors.lastname: Hou
authors.forename: Jin-Mei
authors.initials: JM
authors.lastname: Ma
authors.forename: Guo-Bo
authors.initials: GB
authors.lastname: Quan
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
authors.forename: Lan
authors.initials: L
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D051792:Basic Helix-Loop-Helix Proteins / Q000096:biosynthesis / Q000235:genetics; D002135:Calcium-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D005260:Female; D005854:Germ Cells / Q000254:growth & development / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis / Q000235:genetics; D000072100:Jagged-1 Protein; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D009865:Oocytes / Q000254:growth & development / Q000378:metabolism*; D006080:Ovarian Follicle / Q000254:growth & development / Q000378:metabolism*; D051881:Receptor, Notch1 / Q000096:biosynthesis / Q000235:genetics; D051883:Receptor, Notch2 / Q000096:biosynthesis / Q000235:genetics; D000072099:Serrate-Jagged Proteins; D015398:Signal Transduction / Q000235:genetics; D000072056:Transcription Factor HES-1
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D002135:Calcium-Binding Proteins; D018398:Homeodomain Proteins; D036341:Intercellular Signaling Peptides and Proteins; D000072100:Jagged-1 Protein; D008565:Membrane Proteins; D051881:Receptor, Notch1; D051883:Receptor, Notch2; D000072099:Serrate-Jagged Proteins; D000072056:Transcription Factor HES-1; C489734:Hes1 protein, mouse; C000606274:Jag1 protein, mouse; C497762:Notch1 protein, mouse; C497765:Notch2 protein, mouse