title: [Analysis of protein bodies isolated from Lablab purpureus (L.) Sweet: Intracellular localisation of globulins, proteases and trypsin inhibitors].
abstract: Cotyledonary cells are submitted to fractionation by isopycnic centrifugation. Small intact protein bodies are collected in the densest zones (d=1.205-1.237 g/cm(3)). Fragments of larger bodies are gathered in zones of lower density (d=1.205 g/cm(3)). Small dense bodies are largely sedimentable after dilution, whereas fragments of the large bodies dissociate into a dense sedimentable clot and into floating elements which contain most of the globulins and all of the albumins. Among the dissociated floating components are the BAPA-active endopeptidases and the trypsin inhibitors (BAPA=α-N-benzoyl-DL-arginine-p-nitroanilide). A caseolytic activity remains with the dense mass. The localisation of the albumins, globulins, proteases and trypsin inhibitors is discussed. Relations between solubility, structure and function are considered.
authors.affiliation: Laboratoire de Chimie Taxonomique, Institut de Botanique Systématique de l'Université de Genève, 1. chemin de l'Impératrice, CH-1292, Chambesy, Switzerland.
authors.forename: M N
authors.initials: MN
authors.lastname: Miège
authors.forename: J M
authors.initials: JM
authors.lastname: Mascherpa
authors.forename: A
authors.initials: A
authors.lastname: Royer-Spierer
authors.forename: A
authors.initials: A
authors.lastname: Grange
authors.forename: J
authors.initials: J
authors.lastname: Miège