title: [Gene expression of bone mesenehymal stem cells transduced by the lentiviral vector of SOX9 gene knockdown].
abstract: OBJECTIVE
  To construct one lentiviral vector containing mouse SRY-related silencing group--box gene 9 (SOX9) and to transfect murine bone mesenehymal stem cells (mBMSCs) in vitro and observe the expression of target gene.
  
  
  METHODS
  RNA inteference target sequence was designed in connectin with mice SOX9 gene sequence. The double strands DNAoligo containing interference sequence were synthesized and cloned into lentivirus vector. The siRNA lentiviral vector with SOX9 gene silencing was constructed and identified, which was transfected into rat bone mesenehymal stem cells. The expression of target gene was detected by immunofluorescence, RT-PCR and Western blot.
  
  
  RESULTS
  Lenti-SOX9-siRNA-EGFP was recombined successfully and transduced efficiently into mBMSCs. The expression of SOX9 gene silencing was confirmed by RT-PCR and Western blot.
  
  
  CONCLUSIONS
  Mouse SOX9 gene silencing by RNA interference and Lentiviral vector can transfected successfully into mBMSCs. Meanwhile,SOX9 gene may be silenced in SOX9 transduced mBMSCs. This will provide target cells for the following study about SOX9 gene respairing cartilage injury.
authors.affiliation: Department of Orthopaedics, the Second Hospital Affiliated to Shanxi Medical University, Taiyuan 030001, Shanxi, China.
authors.forename: Da-Chuan
authors.initials: DC
authors.lastname: Liang
authors.affiliation: Department of Orthopaedics, the Second Hospital Affiliated to Shanxi Medical University, Taiyuan 030001, Shanxi, China.
authors.forename: Jie-Yu
authors.initials: JY
authors.lastname: Bai
authors.affiliation: Department of Orthopaedics, the Second Hospital Affiliated to Shanxi Medical University, Taiyuan 030001, Shanxi, China.
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Du
authors.affiliation: Department of Orthopaedics, the Second Hospital Affiliated to Shanxi Medical University, Taiyuan 030001, Shanxi, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Cheng
authors.affiliation: Department of Orthopaedics, the Second Hospital Affiliated to Shanxi Medical University, Taiyuan 030001, Shanxi, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, the Second Hospital Affiliated to Shanxi Medical University, Taiyuan 030001, Shanxi, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Kang
authors.affiliation: Department of Orthopaedics, the Second Hospital Affiliated to Shanxi Medical University, Taiyuan 030001, Shanxi, China.
authors.forename: Zi-Quan
authors.initials: ZQ
authors.lastname: Yang
mesh_terms: D000818:Animals; D005260:Female; D015870:Gene Expression; D020868:Gene Silencing; D015316:Genetic Therapy; D005822:Genetic Vectors; D016086:Lentivirus / Q000235:genetics*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051379:Mice; D055758:SOX9 Transcription Factor / Q000235:genetics*; D014161:Transduction, Genetic
chemical_list: D055758:SOX9 Transcription Factor; C527933:Sox9 protein, mouse