title: Bio-modified carbon nanoparticles loaded with methotrexate possible carrier for anticancer drug delivery.
abstract: The modification of carbon nanoparticles (CNPs) using biological molecules is important in the field of chemical biology, as the CNPs have the potential to deliver the drugs directly to the targeted cells and tissues. We have modified the CNPs by coating bovine serum albumin (BSA) on their surfaces and loaded with methotrexate (Mtx). Infrared spectra have revealed the coating of BSA and Mtx on CNP (CBM). Scanning electron microscopy (SEM) and atomic force microscope (AFM) pictures have exhibited the spherical nature of the composite and coating of the proteins on CNPs. The prepared CBM biocomposite has exhibited a sustained release of drug. MTT assay using A549 lung cancer cell lines has revealed 83% cell death at 150 μg/ml concentration of CBM. These results indicate that CNPs based biocomposites may be tried as therapeutic agents in treatment of cancer like diseases.
authors.affiliation: Bio-Products Laboratory, Central Leather Research Institute, Adyar, Chennai 600 020, Tamil Nadu, India.
authors.forename: Thangavelu
authors.initials: T
authors.lastname: Muthukumar
authors.affiliation: Department of Biotechnology, SRM University, Kattankulathur, Chennai 603 203, India.
authors.forename: Sundaram
authors.initials: S
authors.lastname: Prabhavathi
authors.affiliation: St. Joseph's College of Engineering, Sholinganallur, Chennai 600119, India.
authors.forename: Munusamy
authors.initials: M
authors.lastname: Chamundeeswari
authors.affiliation: Bio-Products Laboratory, Central Leather Research Institute, Adyar, Chennai 600 020, Tamil Nadu, India. Electronic address: sastrytp@hotmail.com.
authors.forename: Thotapalli Parvathaleswara
authors.initials: TP
authors.lastname: Sastry
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002244:Carbon / Q000378:metabolism*; D002417:Cattle; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008727:Methotrexate / Q000494:pharmacology*; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman; D013818:Thermogravimetry; D014961:X-Ray Diffraction
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D012710:Serum Albumin, Bovine; D002244:Carbon; D008727:Methotrexate
keywords: Bovine serum albumin; Cancer; Carbon nanoparticle; Drug delivery; Methotrexate; Sustained release