title: Expression of CagL from Helicobacter pylori and Preliminary Study of its Biological Function.
abstract: Helicobacter pylori (H. pylori) is a highly successful human-specific gastric pathogen, infecting over half the world's population. Virulent H. pylori isolates harbour the cytotoxin-associated genes pathogenicity island (cag-PAI), the majority of which have no known function. In this study, we used cell infection assay and reverse transcriptase PCR, identified that CagL recombinant protein, one of the cag-PAI proteins, induced GES-1 cells to express cytokine IL-8. Then we performed western blot and translocation assay. Our result showed CagL polyclonal antibody counteracted translocation of CagA. This will provide a foundation for the further studies on its biological function.
authors.affiliation: School of Medical Science and Laboratory Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013 Jiangsu People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Medical Science and Laboratory Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013 Jiangsu People's Republic of China.
authors.forename: Shiteng
authors.initials: S
authors.lastname: Huang
authors.affiliation: Department of Clinical Hospital, Jiangsu University, Zhenjiang, 212011 Jiangsu China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Zhao
authors.affiliation: School of Medical Science and Laboratory Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013 Jiangsu People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Han
authors.affiliation: School of Medical Science and Laboratory Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013 Jiangsu People's Republic of China.
authors.forename: Xianwei
authors.initials: X
authors.lastname: Guan
authors.affiliation: School of Medical Science and Laboratory Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013 Jiangsu People's Republic of China.
authors.forename: Shihe
authors.initials: S
authors.lastname: Shao
keywords: CagA; CagL; Helicobacter pylori; T4SS