title: Synergistic effect of ALOX5AP polymorphisms and cigarette smoking on the risk of atherosclerotic cerebral infarction in a Northern Han Chinese population.
abstract: The effect of activating 5-lipoxygenase (ALOX5AP) gene polymorphisms on stroke risk may be influenced by the coexistence of modifiable predisposing conditions. We explored the interactions of ALOX5AP polymorphisms and cigarette smoking in a case-control study of patients with atherosclerotic cerebral infarction (ACI). Three polymorphisms of the ALOX5AP gene (rs10507391, rs4769874, and rs9551963) were analyzed in 420 ACI patients and 488 unrelated healthy controls matched for age and sex from a Northern Han Chinese population. Among the three single nucleotide polymorphisms, only rs10507391 genotype TT/TA was observed to be associated with an increased risk of ACI on multivariate analysis (odds ratio [OR]=1.82, 95% confidence interval [CI]=1.14-2.92, p=0.012) compared with the AA genotype. However, after stratifying by smoking status, multivariate logistic regression analysis revealed that rs10507391 genotype TT/TA and rs9551963 genotype CC/CA had a 5.63-fold (OR=5.63, 95%CI=2.00-15.84, p=0.001) and a 2.71-fold (OR=2.71, 95%CI=1.28-5.73, p=0.009) increased risk for ACI patients who smoked compared with the AA genotype, respectively. Additionally, according to the haplotype analysis, the risk of haplotype TGC (OR=3.12, 95%CI=2.00-4.88, p<0.001, corrected p [pc]<0.001) increased for ACI patients who smoked compared to the data (OR=1.60, 95%CI=1.28-1.98, p<0.001, pc<0.001) in total samples. These results suggest that ALOX5AP polymorphisms are associated with ACI, and cigarette smoking along with ALOX5AP could increase the risk of ACI.
authors.affiliation: Department of Neurology, The Affiliated Hospital of Medical College, Qingdao University, Number 16 Jiangsu Road, Qingdao, Shandong Province 266003, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Yao
authors.affiliation: Department of Neurology, The Affiliated Hospital of Medical College, Qingdao University, Number 16 Jiangsu Road, Qingdao, Shandong Province 266003, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Psychology and Psychiatry, Medical College, Qingdao University, Qingdao, Shandong Province, China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Neurology, The Affiliated Hospital of Medical College, Qingdao University, Number 16 Jiangsu Road, Qingdao, Shandong Province 266003, China.
authors.forename: Rongrong
authors.initials: R
authors.lastname: Yuan
authors.affiliation: Department of Neurology, The Affiliated Hospital of Medical College, Qingdao University, Number 16 Jiangsu Road, Qingdao, Shandong Province 266003, China.
authors.forename: Jianye
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Neurology, The Affiliated Hospital of Medical College, Qingdao University, Number 16 Jiangsu Road, Qingdao, Shandong Province 266003, China.
authors.forename: Fengjiao
authors.initials: F
authors.lastname: Sun
authors.affiliation: Department of Neurology, The Affiliated Hospital of Medical College, Qingdao University, Number 16 Jiangsu Road, Qingdao, Shandong Province 266003, China. Electronic address: zhch0532@sina.com.
authors.forename: Chang
authors.initials: C
authors.lastname: Zhou
mesh_terms: D058927:5-Lipoxygenase-Activating Proteins / Q000235:genetics*; D000368:Aged; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D002544:Cerebral Infarction / Q000208:ethnology / Q000235:genetics*; D005260:Female; D006801:Humans; D002537:Intracranial Arteriosclerosis / Q000208:ethnology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011159:Population Surveillance / Q000379:methods; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects / Q000208:ethnology / Q000235:genetics*
chemical_list: D058927:5-Lipoxygenase-Activating Proteins; C548592:ALOX5AP protein, human
keywords: ALOX5AP; Atherosclerosis; Cigarette smoking; Risk factor; Stroke