title: Subphysiological compressive loading reduces apoptosis following acute impact injury in a porcine cartilage model.
abstract: BACKGROUND
  Acute cartilage injuries induce cell death and are associated with an increased incidence of osteoarthritis development later in life. The objective of this study was to investigate the effect of posttraumatic cyclic compressive loading on chondrocyte viability and apoptosis in porcine articular cartilage plugs.
  
  
  OBJECTIVE
  Compressive loading of acutely injured cartilage can maintain chondrocyte viability by reducing apoptosis after a traumatic impact injury.
  
  
  METHODS
  In vitro controlled laboratory study.
  
  
  METHODS
  Level 5.
  
  
  METHODS
  Each experiment compared 4 test groups: control, impact, impact with compressive loading (either 0.5 or 0.8 MPa), and no impact but compressive loading (n = 15 per group). Flat, full-thickness articular cartilage plugs were harvested from the trochlear region of porcine knees. A drop tower was utilized to introduce an impact injury. The articular plugs were subjected to two 30-minute cycles of either 0.5 or 0.8 MPa of dynamic loading. Cell viability, apoptosis, and gene expression of samples were evaluated 24 hours postimpaction.
  
  
  RESULTS
  Cell viability staining showed that 0.5 MPa of dynamic compressive loading increased cell viability compared with the impact group. Apoptotic analysis revealed a decrease in apoptotic expression in the group with 0.5 MPa of dynamic compressive loading compared with the impact group. Significantly higher caspase 3 and lower collagen II expressions were observed in impacted samples without compressive loading, compared with those with. Compressive loading of nonimpacted samples significantly increased collagen II and decreased caspase 3 expressions.
  
  
  CONCLUSIONS
  In this porcine in vitro model, dynamic compressive loading at subphysiological levels immediately following impact injury decreases apoptotic expression, thereby maintaining chondrocyte viability.
  
  
  CONCLUSIONS
  Therapeutic exercises could be designed to deliver subphysiological loading to the injured cartilage, thereby minimizing injury.
authors.affiliation: Department of Biomedical Engineering, University of Miami, Coral Gables, Florida ; Division of Sports Medicine, UHealth Sports Performance and Wellness Institute, University of Miami Hospital, Miami, Florida.
authors.forename: Lauren
authors.initials: L
authors.lastname: Vernon
authors.affiliation: Department of Biomedical Engineering, University of Miami, Coral Gables, Florida.
authors.forename: Andre
authors.initials: A
authors.lastname: Abadin
authors.affiliation: Division of Sports Medicine, UHealth Sports Performance and Wellness Institute, University of Miami Hospital, Miami, Florida.
authors.forename: David
authors.initials: D
authors.lastname: Wilensky
authors.affiliation: Department of Biomedical Engineering, University of Miami, Coral Gables, Florida.
authors.forename: C-Y Charles
authors.initials: CY
authors.lastname: Huang
authors.affiliation: Department of Biomedical Engineering, University of Miami, Coral Gables, Florida ; Division of Sports Medicine, UHealth Sports Performance and Wellness Institute, University of Miami Hospital, Miami, Florida.
authors.forename: Lee
authors.initials: L
authors.lastname: Kaplan
keywords: cartilage gene expression; chondrocyte viability; compressive loading; impact injury; osteoarthritis prevention