title: Effect of freezing and thawing on cell membranes of Lentinus edodes, the shiitake mushroom.
abstract: When cell membranes of Lentinus edodes mycelium were rapidly frozen at either 50 or 160°C/min, viability was lost and this correlated with rupture of the plasmalemma and residual membrane material and with alterations in the organelles. Although with slow cooling (1°C/min) 80% of the samples recovered viability, some cells still showed similar changes to those cooled rapidly, indicating that individual cells of the mycelium do not respond in the same way.
authors.affiliation: Museum National d'Histoire Naturelle, Laboratoire de Cryptogamie, 12 rue Buffon, 75005, Paris, France.
authors.forename: M F
authors.initials: MF
authors.lastname: Roquebert
authors.forename: E
authors.initials: E
authors.lastname: Bury