title: Correlation between miR-23a and onset of hepatocellular carcinoma.
abstract: OBJECTIVE
  To clarify the role of miR-23a in the onset and development of hepatocarcinoma on the cellular, genetic and molecular levels.
  
  
  METHODS
  Seventy-eight patients were included after hepatectomy. Relationships between the clinical pathological factors of tumor and paracancerous tissues were analyzed. Risk factors of overall and recurrence-free survival rates were subject to multi-variable analysis. Tissues were sequenced by digital miRNA expression profiling, and new miRNA was subject to target gene prediction.
  
  
  RESULTS
  miR-23a expression was correlated with the stage of the TNM Classification of Malignant Tumours most significantly, followed by tumor size (P=0.041 and 0.047). High miR-23a, vascular invasion, tumor size≥7cm, tumor capsule and late pathological stage were the risk factors of overall survival rate, and those of recurrence-free survival rate also included alpha-fetoprotein level≥200μg/L and multiple tumors. Compared with normal hepatic cell line L-02, the miR-23a expression levels in tumor cell lines SMMC-7721 and HepG2 were up-regulated and down-regulated respectively. Transfecting miR-23a inhibitor suppressed cell growth. Apoptotic rates of the control and those transfected with inhibitor-NC and miR-23a inhibitor for 48h were similar.
  
  
  CONCLUSIONS
  High miR-23a expression is the independent prognostic factor of overall and recurrence-free survival rates, and miR-23a may be involved in the onset of hepatocarcinoma as an oncogene.
authors.affiliation: Department of Pharmacy, Affiliated Hospital of Inner Mongolia Medical University, 010059 Hohhot, PR China. Electronic address: lidao_bao@dfci.harvard.edu.
authors.forename: Lidao
authors.initials: L
authors.lastname: Bao
authors.affiliation: Department of Health Care for Cadres, Binzhou People's Hospital, 256610 Binzhou, PR China.
authors.forename: Jianfen
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Respiratory Medicine, Binzhou People's Hospital, 256610 Binzhou, PR China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Dai
authors.affiliation: Department of Pharmacy, Affiliated Hospital of Inner Mongolia Medical University, 010059 Hohhot, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, Affiliated Hospital of Inner Mongolia Medical University, 010059 Hohhot, PR China.
authors.forename: Ruilian
authors.initials: R
authors.lastname: Ma
authors.affiliation: Molecular Biotechnology Center of Inner Mongolia Medical University, 010059 Hohhot, PR China.
authors.forename: Yila
authors.initials: Y
authors.lastname: Su
authors.affiliation: Molecular Biotechnology Center of Inner Mongolia Medical University, 010059 Hohhot, PR China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Cui
authors.affiliation: Department of Hepatobiliary Surgery, Inner Mongolia Medical University, 010059 Hohhot, PR China.
authors.forename: Jianxiang
authors.initials: J
authors.lastname: Niu
authors.affiliation: Kitami Institute of Technology, 090-8507 Kitami, Japan.
authors.forename: Shiming
authors.initials: S
authors.lastname: Bai
authors.affiliation: College of Chemistry and Chemical Engineering, Inner Mongolia University for Nationalities, 028043 Tongliao, PR China.
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: Department of Pathology, Inner Mongolia Medical University, 010059 Hohhot, PR China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Yuan
authors.affiliation: Department of Imaging, Affiliated Hospital of Inner Mongolia Medical University, 010059 Hohhot, PR China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Geriatrics, Affiliated Hospital of Inner Mongolia Medical University, 010059 Hohhot, PR China.
authors.forename: Changqing
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Pharmacy, Affiliated Hospital of Inner Mongolia Medical University, 010059 Hohhot, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Cheng
authors.affiliation: Department of Pharmacy, Affiliated Hospital of Inner Mongolia Medical University, 010059 Hohhot, PR China. Electronic address: renxianhua237@163.com.
authors.forename: Xianhua
authors.initials: X
authors.lastname: Ren
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology*; D002460:Cell Line; D015536:Down-Regulation; D005260:Female; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008099:Liver / Q000473:pathology; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology*; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D011230:Precancerous Conditions / Q000235:genetics / Q000473:pathology; D060888:Real-Time Polymerase Chain Reaction; D012307:Risk Factors; D015854:Up-Regulation; D000509:alpha-Fetoproteins / Q000032:analysis
chemical_list: C552430:MIRN23a microRNA, human; D035683:MicroRNAs; D000509:alpha-Fetoproteins