title: [Butanol production from corn stover hydrolysate with in-situ liquid-liquid extraction].
abstract: Butanol production from corn stover hydrolysates (CSH) with in-situ liquid-liquid extraction was studied to enhance the production and reduce the fermentation cost. Oleyl alcohol was selected as the suitable solvent and added at the initial fermentation time with the ratio of 1:1 (oleyl alcohol: fermentation broth, V/V). Under this condition, butanol and ABE from CSH with 32.1 g/L total sugars were 3.28 and 4.72 g/L, which were 958.1% and 742.9% higher than those of the controls, respectively. Butanol and ABE production from CSH of 49.7 g/L total sugars after detoxification by ion exchange resin D301 coupled with extraction fermentation were 10.34 g/L and 14.72 g/L with an ABE yield of 0.31 g/g (g ABE/g utilized sugar), which were equal to those of glucose and xylose mixture fermentation. The detoxification and extraction fermentation technology of cellulosic butanol production would provide a crucial technical support to the industrialized production of cellulosic butanol.
authors.affiliation: Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, College of Life Science, Henan Agricultural University, Zhengzhou 450002, Henan, China.
authors.forename: Fengqin
authors.initials: F
authors.lastname: Wang
authors.affiliation: Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, College of Life Science, Henan Agricultural University, Zhengzhou 450002, Henan, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, College of Life Science, Henan Agricultural University, Zhengzhou 450002, Henan, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xie
authors.affiliation: Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, College of Life Science, Henan Agricultural University, Zhengzhou 450002, Henan, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, College of Life Science, Henan Agricultural University, Zhengzhou 450002, Henan, China.
authors.forename: Chuanbin
authors.initials: C
authors.lastname: Li
authors.affiliation: Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, College of Life Science, Henan Agricultural University, Zhengzhou 450002, Henan, China.
authors.forename: Andong
authors.initials: A
authors.lastname: Song
mesh_terms: D000440:Butanols / Q000302:isolation & purification / Q000378:metabolism*; D005233:Fatty Alcohols / Q000737:chemistry; D005285:Fermentation; D059625:Liquid-Liquid Extraction / Q000379:methods*; D018547:Plant Stems / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D000440:Butanols; D005233:Fatty Alcohols; C010268:oleyl alcohol