title: The isolation and physiological analysis of mutants of the moss, Physcomitrella patens, which over-produce gametophores.
abstract: Several phenotypically distinct classes of gametophore overproducing mutants have been isolated in P. patens. Mutants belonging to one class resemble the wild-type strain grown on medium containing a high concentration (5-50μM) of exogenously supplied cytokinin. Mutants of this type can increase the production of gametophores in the wildtype strain by cross-feeding it through the culture medium. Mutants belonging to another class resemble the wild-type strain cultured on medium containing a lower concentration (50-500 nM) of exogenous cytokinin. Mutants of this kind cannot cross-feed the wild-type strain through the culture medium. A component, required by the wild-type strain for the initiation of gametophores in response to cytokinin, either is not formed or is not activated in the dark. Gametophore over-producing mutants may also be unable to synthesize/activate this component in the dark and thus, like the wild-type strain, they produce no gametophores in the dark.
authors.affiliation: Department of Genetics, University of Cambridge, CB2 3EH, Cambridge, U.K..
authors.forename: N W
authors.initials: NW
authors.lastname: Ashton
authors.forename: D J
authors.initials: DJ
authors.lastname: Cove
authors.forename: D R
authors.initials: DR
authors.lastname: Featherstone