title: Application of a novel type impinging streams reactor in solid-liquid enzyme reactions and modeling of residence time distribution using GDB model.
abstract: Solid-liquid enzyme reactions constitute important processes in biochemical industries. The isomerization of d-glucose to d-fructose, using the immobilized glucose isomerase (Sweetzyme T), as a typical example of solid-liquid catalyzed reactions has been carried out in one stage and multi-stage novel type of impinging streams reactors. Response surface methodology was applied to determine the effects of certain pertinent parameters of the process namely axial velocity (A), feed concentration (B), nozzles' flow rates (C) and enzyme loading (D) on the performance of the apparatus. The results obtained from the conversion of glucose in this reactor were much higher than those expected in conventional reactors, while residence time was decreased dramatically. Residence time distribution (RTD) in a one-stage impinging streams reactor was investigated using colored solution as the tracer. The results showed that the flow pattern in the reactor was close to that in a continuous stirred tank reactor (CSTR). Based on the analysis of flow region in the reactor, gamma distribution model with bypass (GDB) was applied to study the RTD of the reactor. The results indicated that RTD in the impinging streams reactor could be described by the latter model.
authors.affiliation: Chemical Engineering Department, Amirkabir University of Technology, Tehran, Iran.
authors.forename: Niloufar
authors.initials: N
authors.lastname: Fatourehchi
authors.affiliation: Chemical Engineering Department, Amirkabir University of Technology, Tehran, Iran. Electronic address: sohrabi@aut.ac.ir.
authors.forename: Morteza
authors.initials: M
authors.lastname: Sohrabi
authors.affiliation: Chemical Engineering Department, Amirkabir University of Technology, Tehran, Iran.
authors.forename: Bahram
authors.initials: B
authors.lastname: Dabir
authors.affiliation: Refining Technology Development Division, Research Institute of Petroleum Industry, Tehran, Iran.
authors.forename: Sayed Javid
authors.initials: SJ
authors.lastname: Royaee
authors.affiliation: Chemical Engineering Department, Amirkabir University of Technology, Tehran, Iran.
authors.forename: Adel
authors.initials: A
authors.lastname: Haji Malayeri
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000378:metabolism*; D019149:Bioreactors; D004800:Enzymes, Immobilized; D004867:Equipment Design; D005632:Fructose / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D008962:Models, Theoretical; D012212:Rheology; D013995:Time
chemical_list: D004800:Enzymes, Immobilized; D005632:Fructose; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase; D005947:Glucose
keywords: Enzymatic reactions; Glucose isomerase; Impinging streams reactor; Mixing model; Residence time distribution