title: Improving stability and biocompatibility of alginate/chitosan microcapsule by fabricating bi-functional membrane.
abstract: Cell encapsulation technology holds promise for the cell-based therapy. But poor mechanical strength and biocompatibility of microcapsule membrane are still obstacles for the clinical applications. A novel strategy is presented to prepare AC₁ C₂ A microcapsules with bi-functional membrane (that is, both desirable biocompatibility and membrane stability) by sequentially complexing chitosans with higher deacetylation degree (C₁) and lower deacetylation degree (C₂) on alginate (A) gel beads. Both in vitro and in vivo evaluation of AC₁C₂ A microcapsules demonstrate higher membrane stability and less cell adhesion, because the introduction of C₂ increases membrane strength and decreases surface roughness. Moreover, diffusion test of AC₁C₂ A microcapsules displays no inward permeation of IgG protein suggesting good immunoisolation function. The results demonstrate that AC₁C₂ A microcapsules with bi-functional membrane could be a promising candidate for microencapsulated cell implantation with cost effective usage of naturally biocompatible polysaccharides.
authors.affiliation: Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, P. R. China; University of the Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors.forename: Guoshuang
authors.initials: G
authors.lastname: Zheng
authors.forename: Xiudong
authors.initials: X
authors.lastname: Liu
authors.forename: Xiuli
authors.initials: X
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: Hongguo
authors.initials: H
authors.lastname: Xie
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Huizhen
authors.initials: H
authors.lastname: Zheng
authors.forename: Weiting
authors.initials: W
authors.lastname: Yu
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Ma
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000704:Analysis of Variance; D001672:Biocompatible Materials / Q000378:metabolism*; D001696:Biomechanical Phenomena; D002214:Capsules / Q000737:chemistry* / Q000378:metabolism; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D048271:Chitosan / Q000737:chemistry*; D008567:Membranes, Artificial; D013499:Surface Properties
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D002214:Capsules; D008567:Membranes, Artificial; D048271:Chitosan
keywords: alginate/chitosan microcapsules; bi-functional membranes; biocompatibility mechanical strength; surface roughness