title: Effect of Ocimum basilicum L. on cyclo-oxygenase isoforms and prostaglandins involved in thrombosis.
abstract: BACKGROUND
  Ocimum basilicum L. (OBL) is a plant used in traditional Uyghur medicine for the treatment and prevention of cardiovascular disease. In previous studies we had found an antihypertensive and antithrombotic effect suggestive of an effect on prostaglandins, which we attempt to document here.
  
  
  METHODS
  6-keto-PGF1α, the metabolite of prostacyclin, and PGE2 were measured in the supernatant of human umbilical vein endothelial cells (HUVEC) and basal or LPS-stimulated mouse coeliac macrophage cultures exposed to OBL ethanol (OBL-E) extracts and petroleum ether, chloroform, ethylacetate and butanol (PE, C, EA, B) fractions. In addition, 6-keto-PGF1α and thromboxane B2 (TXB2) were measured in a rat model of thromboangiitis obliterans exposed or not to OBL.
  
  
  RESULTS
  Short-term exposure to OBL-E dose-dependently increased 6-keto-PGF1α from HUVEC, and long-term (24h) exposure decreased it. OBL-C and OBL-B increased 6-keto-PGF1α, whereas the other fractions tended to decrease it after 24h exposure. The extract and all fractions decreased basal and stimulated PGE2 production, but only OBL-EA and OBL-B reduced PGE2 in stimulated cultures to concentrations below the unstimulated values (P<0.05). In vivo OBL increased 6-keto-PGF1α and decreased TXB2.
  
  
  CONCLUSIONS
  OBL and its extracts increased 6-keto-PGF1α and reduced PGE2 and TXB2 production in a dose and time-related manner. This could indicate simultaneous inhibition of COX-2 and stimulation of endothelial COX-1. The butanol fraction seemed most promising in this respect.
authors.affiliation: Department of Pharmacology, Xinjiang Medical University, 393 Xinyi Road, 830011 Urumqi, PR China; Department of Pharmacology, University Bordeaux Segalen, 146 rue Leo Saignat, 33076 Bordeaux, France. Electronic address: anwar.umar@126.com.
authors.forename: Anwar
authors.initials: A
authors.lastname: Umar
authors.affiliation: Department of Pharmacology, Xinjiang Medical University, 393 Xinyi Road, 830011 Urumqi, PR China.
authors.forename: Wenting
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Department of Pharmacology, Xinjiang Medical University, 393 Xinyi Road, 830011 Urumqi, PR China.
authors.forename: Elzira
authors.initials: E
authors.lastname: Abdusalam
authors.affiliation: Medical Research Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang, PR China.
authors.forename: Arzigul
authors.initials: A
authors.lastname: Tursun
authors.affiliation: Department of Cardiovascular Internal Medicine, The Second Affiliated Hospital of Xinjiang Medical University, 38 Nanhu Road, Shuimogou District, 830063 Urumqi, Xinjiang, PR China.
authors.forename: Nadira
authors.initials: N
authors.lastname: Reyim
authors.affiliation: Department of Pharmacology, Xinjiang Medical University, 393 Xinyi Road, 830011 Urumqi, PR China.
authors.forename: Ibadet
authors.initials: I
authors.lastname: Tohti
authors.affiliation: Department of Pharmacology, Xinjiang Medical University, 393 Xinyi Road, 830011 Urumqi, PR China; Department of Pharmacology, University Bordeaux Segalen, 146 rue Leo Saignat, 33076 Bordeaux, France. Electronic address: nicholas.moore@pharmaco.u-bordeaux2.fr.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Moore
mesh_terms: D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D051545:Cyclooxygenase 1 / Q000187:drug effects / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000187:drug effects / Q000378:metabolism; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008519:Medicine, Traditional; D051379:Mice; D018649:Ocimum basilicum / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D011453:Prostaglandins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013919:Thromboangiitis Obliterans / Q000188:drug therapy / Q000473:pathology; D013927:Thrombosis / Q000188:drug therapy* / Q000473:pathology; D013929:Thromboxane B2 / Q000378:metabolism; D013997:Time Factors
chemical_list: D010936:Plant Extracts; D011453:Prostaglandins; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2
keywords: 6-keto-PGF(1)α; HUVEC; In vitro; Macrophage; Ocimum basilicum L.; PGE2