title: Development of "one-pot" method for multi-class compounds in porcine formula feed by multi-function impurity adsorption cleaning followed ultra-performance liquid chromatography-tandem mass spectrometry detection.
abstract: A novel and efficient determination method for multi-class compounds including β-agonists, sedatives, nitro-imidazoles and aflatoxins in porcine formula feed based on a fast "one-pot" extraction/multifunction impurity adsorption (MFIA) clean-up procedure has been developed. 23 target analytes belonging to four different class compounds could be determined simultaneously in a single run. Conditions for "one-pot" extraction were studied in detail. Under the optimized conditions, the multi-class compounds in porcine formula feed samples were extracted and purified with methanol contained ammonia and absorbents by one step. The compounds in extracts were purified by using multi types of absorbent based on MFIA in one pot. The multi-walled carbon nanotubes were employed to improved clean-up efficiency. Shield BEH C18 column was used to separate 23 target analytes, followed by tandem mass spectrometry (MS/MS) detection using an electro-spray ionization source in positive mode. Recovery studies were done at three fortification levels. Overall average recoveries of target compounds in porcine formula feed at each levels were >51.6% based on matrix fortified calibration with coefficients of variation from 2.7% to 13.2% (n=6). The limit of determination (LOD) of these compounds in porcine formula feed sample matrix was <5.0 μg/kg. This method was successfully applied in screening and confirmation of target drugs in >30 porcine formula feed samples. It was demonstrated that the integration of the MFIA protocol with the MS/MS instrument could serve as a valuable strategy for rapid screening and reliable confirmatory analysis of multi-class compounds in real samples.
authors.affiliation: Key Laboratory of Agro-product Safety and Quality, Ministry of Agriculture, Beijing 100081, PR China; Institute of Quality Standards & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Peilong
authors.initials: P
authors.lastname: Wang
authors.affiliation: Key Laboratory of Agro-product Safety and Quality, Ministry of Agriculture, Beijing 100081, PR China; Institute of Quality Standards & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Agro-product Safety and Quality, Ministry of Agriculture, Beijing 100081, PR China; Institute of Quality Standards & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Agro-product Safety and Quality, Ministry of Agriculture, Beijing 100081, PR China; Institute of Quality Standards & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China. Electronic address: suxiaoou@caas.cn.
authors.forename: Xiaoou
authors.initials: X
authors.lastname: Su
mesh_terms: D000327:Adsorption; D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination / Q000032:analysis*; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D013552:Swine; D053719:Tandem Mass Spectrometry
chemical_list: D004364:Pharmaceutical Preparations
keywords: Multi-class compounds; Multi-function impurity adsorption; Porcine formula feed; Ultra performance liquid chromatography–tandem mass spectrometry