title: A validated GC/MS method for the determination of amisulpride in whole blood.
abstract: A sensitive GC/MS method for the determination of amisulpride in whole blood was developed, optimized and validated. Sample preparation included solid-phase extraction using HF Bond Elut C18 cartridges and further derivatization with heptafluorobutyric anhydride (HFBA). The limits of detection and quantification were 3.00 and 10.0 μg/L, respectively. The calibration curves were linear up to 1000 μg/L (R(2)≥0.991). Absolute recovery ranged from 94.2 to 101%. Accuracy was found to be between -8.7 and 1.9% and imprecision was less than 10.0%. The developed method covers the generally accepted therapeutic range but it can also cover levels above them. This makes our method suitable for the determination of amisulpride not only for clinical purposes on psychiatric patients, but also during the investigation of forensic cases where amisulpride is involved.
authors.affiliation: Department of Forensic Medicine and Toxicology, Faculty of Medicine, National and Kapodistrian University of Athens, 75 Mikras Asias, Athens 115 27, Greece.
authors.forename: Ioannis
authors.initials: I
authors.lastname: Papoutsis
authors.affiliation: Department of Forensic Medicine and Toxicology, Faculty of Medicine, National and Kapodistrian University of Athens, 75 Mikras Asias, Athens 115 27, Greece.
authors.forename: Anna
authors.initials: A
authors.lastname: Rizopoulou
authors.affiliation: Department of Forensic Medicine and Toxicology, Faculty of Medicine, National and Kapodistrian University of Athens, 75 Mikras Asias, Athens 115 27, Greece. Electronic address: pan_nik@hotmail.com.
authors.forename: Panagiota
authors.initials: P
authors.lastname: Nikolaou
authors.affiliation: Department of Forensic Medicine and Toxicology, Faculty of Medicine, National and Kapodistrian University of Athens, 75 Mikras Asias, Athens 115 27, Greece.
authors.forename: Constantinos
authors.initials: C
authors.lastname: Pistos
authors.affiliation: Department of Forensic Medicine and Toxicology, Faculty of Medicine, National and Kapodistrian University of Athens, 75 Mikras Asias, Athens 115 27, Greece.
authors.forename: Chara
authors.initials: C
authors.lastname: Spiliopoulou
authors.affiliation: Department of Forensic Medicine and Toxicology, Faculty of Medicine, National and Kapodistrian University of Athens, 75 Mikras Asias, Athens 115 27, Greece.
authors.forename: Sotiris
authors.initials: S
authors.lastname: Athanaselis
mesh_terms: D000328:Adult; D000368:Aged; D000077582:Amisulpride; D014150:Antipsychotic Agents / Q000097:blood*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D013469:Sulpiride / Q000031:analogs & derivatives* / Q000097:blood
chemical_list: D014150:Antipsychotic Agents; D013469:Sulpiride; D000077582:Amisulpride
keywords: Amisulpride; Blood; GC/MS