title: Androgen deficiency during mid- and late pregnancy alters progesterone production and metabolism in the porcine corpus luteum.
abstract: We determined whether androgen deficiency induced by flutamide treatment during mid- and late pregnancy affects the functions of the porcine corpus luteum (CL). Pregnant gilts were injected with flutamide between days 43 and 49 (gestation day [GD] 50F), days 83 and 89 (GD90F), or days 101 and 107 (GD108F) of gestation. Antiandrogen treatment increased the luteal progesterone concentration in the GD50F group and decreased progesterone content in the GD90F and GD108F groups. Luteal levels of side-chain cleavage cytochrome P450 (CYP11A1) mRNA and protein were significantly downregulated in the GD90F and GD108F groups as compared with the respective controls. The 3β-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase (HSD3B) mRNA and protein expression were significantly reduced only in the GD108F group as compared with the control. Decreased luteal 20α-hydroxysteroid dehydrogenase (AKR1C1) mRNA and protein levels were observed in the GD50F group. Thus, androgen deficiency during pregnancy in pigs led to CL dysfunction that is marked by decreased progesterone production. Furthermore, exposure to flutamide during late pregnancy downregulated steroidogenic enzymes (CYP11A1 and HSD3B) in pigs. We conclude that androgens are important regulators of CL function during pregnancy.
authors.affiliation: 1Department of Endocrinology, Institute of Zoology, Jagiellonian University in Krakow, Krakow, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Grzesiak
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Knapczyk-Stwora
authors.forename: Renata E
authors.initials: RE
authors.lastname: Ciereszko
authors.forename: Aniela
authors.initials: A
authors.lastname: Golas
authors.forename: Iwona
authors.initials: I
authors.lastname: Wieciech
authors.forename: Maria
authors.initials: M
authors.lastname: Slomczynska
mesh_terms: D000726:Androgen Antagonists / Q000494:pharmacology; D000728:Androgens / Q000172:deficiency*; D000818:Animals; D003338:Corpus Luteum / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D005485:Flutamide / Q000494:pharmacology; D011247:Pregnancy / Q000187:drug effects / Q000378:metabolism*; D011374:Progesterone / Q000096:biosynthesis*; D011897:Random Allocation; D013552:Swine
chemical_list: D000726:Androgen Antagonists; D000728:Androgens; D011374:Progesterone; D005485:Flutamide
keywords: corpus luteum; flutamide; pig; pregnancy; progesterone