title: Adipose-derived stromal vascular fraction cells and platelet-rich plasma: basic and clinical evaluation for cell-based therapies in patients with scars on the face.
abstract: BACKGROUND
  Actually, autologous fat grafts have many clinical applications in breast surgery, facial rejuvenation, buttock augmentation, and Romberg syndrome as well as a treatment of liposuction sequelae.
  
  
  OBJECTIVE
  The aim of this article was to describe the preparation and isolation procedures for stromal vascular fraction (SVF), the preparation of platelet-rich plasma (PRP), and the clinical application in the treatment of the scar on the face.
  
  
  METHODS
  Ten patients with burns sequelae (n = 6) and post-traumatic scars (n = 4) were treated with SVF-enhanced autologous fat grafts obtained by the Celution System. Another 10 patients with burns sequelae (n = 5) and post-traumatic scars (n = 5) were treated with fat grafting based on the Coleman technique mixed with 0.5 mL of PRP.To assess the effects of their treatment, the authors compared their results with those of a control group consisting of 10 patients treated with centrifuged fat.
  
  
  RESULTS
  In the patients treated with SVF-enhanced autologous fat grafts, we observed a 63% maintenance of contour restoring after 1 year compared with only 39% of the control group (n = 10) treated with centrifuged fat graft (P < 0.0001). In the patients treated with fat grafting and PRP, we observed a 69% maintenance of contour restoring after 1 year compared with that of the control group (n = 10).
  
  
  CONCLUSIONS
  Autologous fat grafting is a good method for the correction of scars on the face instead of the traditional scar surgical excision.
authors.affiliation: From the *Department of Plastic and Reconstructive Surgery, University of Rome Tor Vergata; †Department of Immunohematology and Transfusion, Casilino Hospital; and ‡Department of Education, University of Rome Tor Vergata, Rome, Italy.
authors.forename: Pietro
authors.initials: P
authors.lastname: Gentile
authors.forename: Barbara
authors.initials: B
authors.lastname: De Angelis
authors.forename: Methap
authors.initials: M
authors.lastname: Pasin
authors.forename: Giulio
authors.initials: G
authors.lastname: Cervelli
authors.forename: Cristiano B
authors.initials: CB
authors.lastname: Curcio
authors.forename: Micol
authors.initials: M
authors.lastname: Floris
authors.forename: Camilla
authors.initials: C
authors.lastname: Di Pasquali
authors.forename: Ilaria
authors.initials: I
authors.lastname: Bocchini
authors.forename: Alberto
authors.initials: A
authors.lastname: Balzani
authors.forename: Fabio
authors.initials: F
authors.lastname: Nicoli
authors.forename: Chiara
authors.initials: C
authors.lastname: Insalaco
authors.forename: Eleonora
authors.initials: E
authors.lastname: Tati
authors.forename: Lucilla
authors.initials: L
authors.lastname: Lucarini
authors.forename: Ludovico
authors.initials: L
authors.lastname: Palla
authors.forename: Michele
authors.initials: M
authors.lastname: Pascali
authors.forename: Pamela
authors.initials: P
authors.lastname: De Logu
authors.forename: Chiara
authors.initials: C
authors.lastname: Di Segni
authors.forename: Davide J
authors.initials: DJ
authors.lastname: Bottini
authors.forename: Valerio
authors.initials: V
authors.lastname: Cervelli
mesh_terms: D017667:Adipocytes / Q000637:transplantation*; D000273:Adipose Tissue / Q000166:cytology* / Q000637:transplantation; D000328:Adult; D000368:Aged; D064592:Autografts / Q000637:transplantation; D002056:Burns / Q000601:surgery; D002921:Cicatrix / Q000601:surgery*; D005145:Face / Q000601:surgery*; D005151:Facial Injuries / Q000601:surgery; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008875:Middle Aged; D039902:Multipotent Stem Cells / Q000637:transplantation; D017060:Patient Satisfaction; D053657:Platelet-Rich Plasma / Q000502:physiology*; D019651:Plastic Surgery Procedures / Q000379:methods*; D012038:Regeneration / Q000502:physiology; D017154:Stromal Cells / Q000637:transplantation; D016896:Treatment Outcome; D055815:Young Adult