title: Miro1 regulates intercellular mitochondrial transport & enhances mesenchymal stem cell rescue efficacy.
abstract: There is emerging evidence that stem cells can rejuvenate damaged cells by mitochondrial transfer. Earlier studies show that epithelial mitochondrial dysfunction is critical in asthma pathogenesis. Here we show for the first time that Miro1, a mitochondrial Rho-GTPase, regulates intercellular mitochondrial movement from mesenchymal stem cells (MSC) to epithelial cells (EC). We demonstrate that overexpression of Miro1 in MSC (MSCmiro(Hi)) leads to enhanced mitochondrial transfer and rescue of epithelial injury, while Miro1 knockdown (MSCmiro(Lo)) leads to loss of efficacy. Treatment with MSCmiro(Hi) was associated with greater therapeutic efficacy, when compared to control MSC, in mouse models of rotenone (Rot) induced airway injury and allergic airway inflammation (AAI). Notably, airway hyperresponsiveness and remodeling were reversed by MSCmiro(Hi) in three separate allergen-induced asthma models. In a human in vitro system, MSCmiro(Hi) reversed mitochondrial dysfunction in bronchial epithelial cells treated with pro-inflammatory supernatant of IL-13-induced macrophages. Anti-inflammatory MSC products like NO, TGF-β, IL-10 and PGE2, were unchanged by Miro1 overexpression, excluding non-specific paracrine effects. In summary, Miro1 overexpression leads to increased stem cell repair.
authors.affiliation: Centre of Excellence in Asthma & Lung Disease, CSIR-Institute of Genomics and Integrative Biology, Delhi, India.
authors.forename: Tanveer
authors.initials: T
authors.lastname: Ahmad
authors.forename: Shravani
authors.initials: S
authors.lastname: Mukherjee
authors.forename: Bijay
authors.initials: B
authors.lastname: Pattnaik
authors.forename: Manish
authors.initials: M
authors.lastname: Kumar
authors.forename: Suchita
authors.initials: S
authors.lastname: Singh
authors.forename: Manish
authors.initials: M
authors.lastname: Kumar
authors.forename: Rakhshinda
authors.initials: R
authors.lastname: Rehman
authors.forename: Brijendra K
authors.initials: BK
authors.lastname: Tiwari
authors.forename: Kumar A
authors.initials: KA
authors.lastname: Jha
authors.forename: Amruta P
authors.initials: AP
authors.lastname: Barhanpurkar
authors.forename: Mohan R
authors.initials: MR
authors.lastname: Wani
authors.forename: Soumya S
authors.initials: SS
authors.lastname: Roy
authors.forename: Ulaganathan
authors.initials: U
authors.lastname: Mabalirajan
authors.forename: Balaram
authors.initials: B
authors.lastname: Ghosh
authors.forename: Anurag
authors.initials: A
authors.lastname: Agrawal
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000235:genetics; D002478:Cells, Cultured; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D008168:Lung / Q000473:pathology; D055370:Lung Injury / Q000473:pathology / Q000628:therapy*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D008928:Mitochondria / Q000378:metabolism* / Q000637:transplantation; D041681:NIH 3T3 Cells; D043942:Nanotubes; D016896:Treatment Outcome; D020741:rho GTP-Binding Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: C573782:Miro-1 protein, mouse; D020741:rho GTP-Binding Proteins