title: Phosphorylation of alfalfa mosaic virus movement protein in vivo.
abstract: The 32-kDa movement protein, P3, of alfalfa mosaic virus (AMV) is essential for cell-to-cell spread of the virus in plants. P3 shares many properties with other virus movement proteins (MPs); however, it is not known if P3 is posttranslationally modified by phosphorylation, which is important for the function of other MPs. When expressed in Nicotiana tabacum, P3 accumulated primarily in the cell walls of older leaves or in the cytosol of younger leaves. When expressed in Pischia pastoris, P3 accumulated primarily in a soluble form. Metabolic labeling indicated that a portion of P3 was phosphorylated in both tobacco and yeast, suggesting that phosphorylation regulates the function of this protein as it does for other virus MPs.
authors.affiliation: Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN, 47907, USA.
authors.forename: Bong-Suk
authors.initials: BS
authors.lastname: Kim
authors.forename: Edward L
authors.initials: EL
authors.lastname: Halk
authors.forename: Donald J
authors.initials: DJ
authors.lastname: Merlo
authors.forename: Steven E
authors.initials: SE
authors.lastname: Nelson
authors.forename: L Sue
authors.initials: LS
authors.lastname: Loesch-Fries
mesh_terms: D017794:Alfalfa mosaic virus / Q000235:genetics / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D010766:Phosphorylation / Q000502:physiology; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves; D053830:Plant Viral Movement Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D012441:Saccharomyces cerevisiae; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism
chemical_list: D053830:Plant Viral Movement Proteins