title: Changes in rRNA transcription influence proliferation and cell fate within a stem cell lineage.
abstract: Ribosome biogenesis drives cell growth and proliferation, but mechanisms that modulate this process within specific lineages remain poorly understood. Here, we identify a Drosophila RNA polymerase I (Pol I) regulatory complex composed of Under-developed (Udd), TAF1B, and a TAF1C-like factor. Disruption of udd or TAF1B results in reduced ovarian germline stem cell (GSC) proliferation. Female GSCs display high levels of ribosomal RNA (rRNA) transcription, and Udd becomes enriched in GSCs relative to their differentiating daughters. Increasing Pol I transcription delays differentiation, whereas reducing rRNA production induces both morphological changes that accompany multicellular cyst formation and specific decreased expression of the bone morphogenetic protein (BMP) pathway component Mad. These findings demonstrate that modulating rRNA synthesis fosters changes in the cell fate, growth, and proliferation of female Drosophila GSCs and their daughters.
authors.affiliation: Department of Molecular Biology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390-9148, USA.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Zhang
authors.forename: Nevine A
authors.initials: NA
authors.lastname: Shalaby
authors.forename: Michael
authors.initials: M
authors.lastname: Buszczak
mesh_terms: D000818:Animals; D019070:Cell Lineage / Q000235:genetics*; D049109:Cell Proliferation; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000166:cytology* / Q000235:genetics; D005260:Female; D020459:Genes, rRNA; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D010053:Ovary / Q000166:cytology / Q000502:physiology*; D012318:RNA Polymerase I / Q000235:genetics / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*; D035381:TATA-Binding Protein Associated Factors; D035362:Transcription Factor TFIID / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D029721:Drosophila Proteins; D009687:Nuclear Proteins; C586090:TAF1B protein, Drosophila; C586091:TAF1C-like protein, Drosophila; D035381:TATA-Binding Protein Associated Factors; D035362:Transcription Factor TFIID; D014157:Transcription Factors; C586089:Udd protein, Drosophila; D051548:Histone Acetyltransferases; C069001:TATA-binding protein associated factor 250 kDa; D012318:RNA Polymerase I