title: [Effects of intra-arterial infusion of 3-bromopyruvate on metastases and survival benefit of hepatic VX2 tumor in rabbits].
abstract: OBJECTIVE
  To evaluate the metastasis and survival of an intra-arterial infusion of 3-bromopyruvate (3-BrPA) on hepatic VX2 tumor in rabbits.
  
  
  METHODS
  VX2 tumor was implanted in left lateral lobe of liver of 18 white New Zealand rabbits. The animals were randomized into 3 groups (n = 6 each) and underwent an intra-arterial infusion of phosphate-buffered saline or 3-BrPA via hepatic artery at 14 days post-implantation. At 28 days post-implantation, 3 rabbits in each group were sacrificed. The abdomen of these rabbits was opened and inspected for metastases. Then the survival of the remaining rabbits was observed.
  
  
  RESULTS
  At 28 days post-implantation, in PBS group, there were intrahepatic metastasis and abdominal cavity dissemination (n = 3), renal metastases (n = 2) and lung metastases (n = 2); in early 3-BrPA infusion group, intrahepatic metastasis (n = 2), abdominal cavity dissemination (n = 1) and lung metastases (n = 1); in late 3-BrPA infusion group, intrahepatic metastasis (n = 1) and lung metastases (n = 1). The survival of the remaining animals was observed. Rabbits in early 3-BrPA infusion group survived significantly longer than those in PBS group [(27 ± 5) vs (17 ± 3) days, P = 0.041]; rabbits in late 3-BrPA infusion group [(42 ± 6) days] survived significantly longer than those in early 3-BrPA infusion group (P = 0.007).
  
  
  CONCLUSIONS
  An intra-arterial infusion of 3-BrPA could reduce metastasis and prolong survival in rabbits with hepatic VX2 tumor. The earlier the infusion, the better the outcome.
authors.affiliation: State Key Laboratory of Oncology in Southern China; Department of Medical Imaging & Interventional Radiology, Cancer Center, Sun Yat-sen University, Guangzhou 510060, China.
authors.forename: Xiong-ying
authors.initials: XY
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Oncology in Southern China; Department of Medical Imaging & Interventional Radiology, Cancer Center, Sun Yat-sen University, Guangzhou 510060, China.
authors.forename: Xiao-ping
authors.initials: XP
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Oncology in Southern China; Department of Medical Imaging & Interventional Radiology, Cancer Center, Sun Yat-sen University, Guangzhou 510060, China. Email: hjinh@mail.sysu.edu.cn.
authors.forename: Jin-hua
authors.initials: JH
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Oncology in Southern China; Department of Medical Imaging & Interventional Radiology, Cancer Center, Sun Yat-sen University, Guangzhou 510060, China.
authors.forename: Rong-guang
authors.initials: RG
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Oncology in Southern China; Department of Medical Imaging & Interventional Radiology, Cancer Center, Sun Yat-sen University, Guangzhou 510060, China.
authors.forename: Bi-jian
authors.initials: BJ
authors.lastname: Miao
authors.affiliation: State Key Laboratory of Oncology in Southern China; Department of Medical Imaging & Interventional Radiology, Cancer Center, Sun Yat-sen University, Guangzhou 510060, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D008114:Liver Neoplasms, Experimental / Q000401:mortality* / Q000473:pathology*; D009362:Neoplasm Metastasis; D011773:Pyruvates / Q000494:pharmacology*; D011817:Rabbits; D016019:Survival Analysis
chemical_list: D011773:Pyruvates; C017092:bromopyruvate