title: Grape-seed procyanidins prevent the cafeteria-diet-induced decrease of glucagon-like peptide-1 production.
abstract: Grape-seed procyanidin extract (GSPE) has been reported to improve insulin resistance in cafeteria rats. Because glucagon-like peptide-1 (GLP-1) is involved in glucose homeostasis, the preventive effects of GSPE on GLP-1 production, secretion, and elimination were evaluated in a model of diet-induced insulin resistance. Rats were fed a cafeteria diet for 12 weeks, and 25 mg of GSPE/kg of body weight was administered concomitantly. Vehicle-treated cafeteria-fed rats and chow-fed rats were used as controls. The cafeteria diet decreased active GLP-1 plasma levels, which is attributed to a decreased intestinal GLP-1 production, linked to reduced colonic enteroendocrine cell populations. Such effects were prevented by GSPE. In the same context, GSPE avoided the decrease on intestinal dipeptidyl-peptidase 4 (DPP4) activity and modulated the gene expression of GLP-1 and its receptor in the hypothalamus. In conclusion, the preventive treatment with GSPE abrogates the effects of the cafeteria diet on intestinal GLP-1 production and DPP4 activity.
authors.affiliation: Departament de Bioquímica i Biotecnologia, Universitat Rovira i Virgili , 43007 Tarragona, Spain.
authors.forename: Noemi
authors.initials: N
authors.lastname: González-Abuín
authors.forename: Neus
authors.initials: N
authors.lastname: Martínez-Micaelo
authors.forename: Mayte
authors.initials: M
authors.lastname: Blay
authors.forename: Anna
authors.initials: A
authors.lastname: Ardévol
authors.forename: Montserrat
authors.initials: M
authors.lastname: Pinent
mesh_terms: D000818:Animals; D044946:Biflavonoids / Q000378:metabolism*; D002392:Catechin / Q000378:metabolism*; D004032:Diet / Q000009:adverse effects*; D015536:Down-Regulation; D005260:Female; D052216:Glucagon-Like Peptide 1 / Q000235:genetics / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D056604:Grape Seed Extract / Q000378:metabolism*; D006801:Humans; D007333:Insulin Resistance; D044945:Proanthocyanidins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D027843:Vitis / Q000737:chemistry / Q000378:metabolism*
chemical_list: D044946:Biflavonoids; D056604:Grape Seed Extract; D044945:Proanthocyanidins; C017674:procyanidin; D052216:Glucagon-Like Peptide 1; D002392:Catechin; D005947:Glucose