title: Comparative proteomic analysis of green malts between barley (Hordeum vulgare) cultivars.
abstract: The variety of metabolic proteins theoretically reaches a peak at the end of germination in large-scale malting. In the present study, comparative proteomics based on two-dimensional fluorescent difference gel electrophoresis (2D-DIGE) was employed to quantitatively analyse the low-salt soluble proteins in green malts from cultivars of Dan'er and Metcalfe. Fifty-nine metabolic proteins with significant differences between cultivars were successfully identified using MALDI-TOF/TOF. The roles of differential proteins in malt quality discrimination were elucidated according to their functions. Among them, 18 proteins exhibited differences in the green malts but not in the malts between the two cultivars. They could be considered as supplementary contributors to the quality defects of Dan'er malt, and new markers for malt quality improvement.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Jin
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China.
authors.forename: Guo-Lin
authors.initials: GL
authors.lastname: Cai
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China.
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Li
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Yang
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China; Industrial Technology Research Institute of Jiangnan University in Suqian, 888 Renmin Road, Suqian 223800, China. Electronic address: jlu@jiangnan.edu.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Biological Fermentation Engineering of Beer, Technical Center of Tsingtao Brewery Co. Ltd., Qingdao 266061, China.
authors.forename: Jian-jun
authors.initials: JJ
authors.lastname: Dong
mesh_terms: D018525:Germination; D001467:Hordeum / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods; D010940:Plant Proteins / Q000378:metabolism; D040901:Proteomics
chemical_list: D010940:Plant Proteins
keywords: 2D-DIGE; Barley cultivars; Comparative proteomics; Green malt; Malt quality; Tandem mass spectrometry