title: Enhanced cell growth by nanoengineering zirconia to stimulate electrostatic fibronectin activation.
abstract: We address the enhanced bone growth on designed nanocrystalline zirconia implants as reported by in vivo experiments. In vitro experiments demonstrate that the activation of adhesive proteins on nanoengineered zirconia stimulates cell adhesion and growth as shown by confocal microscopy. Fibrillar fibronectin (FN) forms a matrix assembly on the nanostructured surface in the cell adhesion process. We discuss the importance of FN dimer activation due to its immobilization on the designed nanocrystalline ZrO2 implant fabricated by ion beam assisted deposition. The Monte-Carlo analysis indicates that FN activation on the surface can be promoted by selective electrostatic interactions between negatively charged ZrO2 surface patches and oppositely charged FN domains.
authors.affiliation: Department of Orthopaedic Surgery and Rehabilitation, UNMC, Omaha, NE 68198, USA.
authors.forename: A I
authors.initials: AI
authors.lastname: Rubinstein
authors.forename: R F
authors.initials: RF
authors.lastname: Sabirianov
authors.forename: F
authors.initials: F
authors.lastname: Namavar
mesh_terms: D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials; D001854:Bone Marrow Cells / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects*; D002462:Cell Membrane / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D003198:Computer Simulation; D003460:Crystallization; D019281:Dimerization; D005353:Fibronectins / Q000737:chemistry*; D018613:Microscopy, Confocal; D009010:Monte Carlo Method; D036103:Nanotechnology / Q000379:methods*; D017434:Protein Structure, Tertiary; D051381:Rats; D055672:Static Electricity; D017154:Stromal Cells / Q000187:drug effects; D013499:Surface Properties; D015040:Zirconium / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D005353:Fibronectins; D015040:Zirconium; C028541:zirconium oxide