title: In Vitro Characterization of a Recombinant AHL-Lactonase from Bacillus cereus Isolated from a Striped Catfish (Pangasianodon hypophthalmus) Pond.
abstract: aiiA gene encoding AHL-lactonase was isolated from Bacillus cereus strain N26.2, originating from a striped catfish (Pangasianodon hypophthalmus) pond in Vietnam. This gene, abbreviated as aiiA(N26.2), was cloned and expressed in a competent Escherichia coli strain BL21(DE3)pLysS. The resulting protein, abbreviated as AiiAN26.2, was highly active in the pH range of 6-8 and could retain 80 % of the maximum activity under storage for 5 days at 4 °C or for 3 days at 20 °C. These properties of AiiAN26.2 protein confers its future application via feed supplementation, with the purpose of controlling aquaculture pathogens which regulate the virulence via a quorum sensing system.
authors.affiliation: Research Institute for Aquaculture No. 2, 116 Nguyen Dinh Chieu, District 1, Ho Chi Minh City, Vietnam.
authors.forename: Nguyen Thi Ngoc
authors.initials: NT
authors.lastname: Tinh
authors.affiliation: Research Institute for Aquaculture No. 2, 116 Nguyen Dinh Chieu, District 1, Ho Chi Minh City, Vietnam.
authors.forename: Nguyen Viet
authors.initials: NV
authors.lastname: Dung
authors.affiliation: Research Institute for Aquaculture No. 2, 116 Nguyen Dinh Chieu, District 1, Ho Chi Minh City, Vietnam.
authors.forename: Cao Thanh
authors.initials: CT
authors.lastname: Trung
authors.affiliation: Research Institute for Aquaculture No. 2, 116 Nguyen Dinh Chieu, District 1, Ho Chi Minh City, Vietnam.
authors.forename: Van Thi
authors.initials: VT
authors.lastname: Thuy
keywords: AHL-lactonase activity; AHL-mediated quorum sensing; Bacillus cereus; Recombinant AHL-lactonase; aiiA gene