title: Minor components of olive oil facilitate the triglyceride clearance from postprandial lipoproteins in a polarity-dependent manner in healthy men.
abstract: Postprandial triglyceride-rich lipoproteins (TRLs) are recognized as atherogenic particles whose lipid composition and function can be modified by the composition of dietary oils. This study was designed to test the hypothesis that minor components of pomace olive oil (POMACE) can not only change the composition of postprandial TRL but also affect the clearance of triglyceride (TG) molecular species of postprandial TRL. Meals enriched in either POMACE or refined olive oil (OLIVE) were administered to 10 healthy young men. TRL were isolated from serum at 2, 4, and 6 hours postprandially, and their fatty acid and TG molecular species compositions were analyzed by gas chromatography. The apolipoprotein B concentration was determined by immunoturbidimetry. POMACE and OLIVE, differing mainly in their unsaponifiable fraction, led to similar fatty acid and TG molecular species profiles in postprandial TRL. However, POMACE-TRL presented a higher particle size, estimated as TG to apolipoprotein B ratio, which was also found for the main TG molecular species (trioleoyl-glycerol, palmitoyl-dioleoyl-glycerol, palmitoyl-oeloyl-linoleoyl-glycerol, and dioleoyl-linoleoyl-glycerol). TG from POMACE-TRL also showed higher clearance rates. In this regard, apolar TG (with a higher equivalent carbon number) disappeared more rapidly from TRL particles obtained after the ingestion of either POMACE or OLIVE. In conclusion, minor components of POMACE facilitated TG clearance from TRL by modifying their particle size and the hydrolysis of the most apolar species.
authors.affiliation: Nutrition and Lipid Metabolism, Instituto de la Grasa (CSIC), 41012 Seville, Spain.
authors.forename: Rosana
authors.initials: R
authors.lastname: Cabello-Moruno
authors.affiliation: Nutrition and Lipid Metabolism, Instituto de la Grasa (CSIC), 41012 Seville, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Martinez-Force
authors.affiliation: Emergencies Services, General Hospital, HHUU Virgen del Rocío, 41013 Seville, Spain.
authors.forename: Emilio
authors.initials: E
authors.lastname: Montero
authors.affiliation: Nutrition and Lipid Metabolism, Instituto de la Grasa (CSIC), 41012 Seville, Spain. Electronic address: perona@ig.csic.es.
authors.forename: Javier S
authors.initials: JS
authors.lastname: Perona
mesh_terms: D000328:Adult; D001055:Apolipoproteins B / Q000097:blood; D015992:Body Mass Index; D005227:Fatty Acids / Q000032:analysis / Q000097:blood; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008297:Male; D062407:Meals; D008657:Metabolic Clearance Rate; D000069463:Olive Oil; D010316:Particle Size; D010938:Plant Oils / Q000008:administration & dosage* / Q000737:chemistry*; D019518:Postprandial Period; D014280:Triglycerides / Q000097:blood*
chemical_list: D001055:Apolipoproteins B; D005227:Fatty Acids; D008074:Lipoproteins; D000069463:Olive Oil; D010938:Plant Oils; D014280:Triglycerides
keywords: ANOVA; Apolipoprotein B; ECN; Fatty acid; GC; Human; LDL; LPL; Lipoprotein; MUFA; OLIVE; OLL; OOL; OOO; Olive oil; POL; POMACE; POO; SOL; SOO; TG; TRL; Triglyceride; Triolein; VLDL; analysis of variance; apo; apolipoprotein; dioleoyl-linoleoyl-glycerol; equivalent carbon number; gas chromatography; lipoprotein lipase; low-density lipoprotein; monounsaturated fatty acids; oleoyl-dilinoleoyl-glycerol; palmitoyl-dioleoyl-glycerol; palmitoyl-oleoyl-linoleoyl-glycerol; pomace olive oil; refined olive oil; stearoyl-dioleoyl-glycerol; stearoyl-oleoyl-linoleoyl-glycerol; triglyceride; triglyceride-rich lipoprotein; trioleoyl-glycerol; very low-density lipoprotein.