title: Sera of overweight people promote in vitro adipocyte differentiation of bone marrow stromal cells.
abstract: BACKGROUND
  Overweight status should not be considered merely an aesthetic concern; rather, it can incur health risks since it may trigger a cascade of events that produce further fat tissue through altered levels of circulating signaling molecules.
  
  
  METHODS
  We decided to investigate the influence of overweight individuals' sera on in vitro MSC proliferation and differentiation.
  
  
  RESULTS
  We observed that in vitro incubation of bone marrow stromal cells with the sera of overweight individuals promotes the adipogenic differentiation of MSCs while partially impairing proper osteogenesis.
  
  
  CONCLUSIONS
  These results, which represent a pilot study, might suggest that becoming overweight triggers further weight gains by promoting a bias in the differentiation potential of MSCs toward adipogenesis. The circulating factors involved in this phenomenon remain to be determined, since the great majority of the well known pro-inflammatory cytokines and adipocyte-secreted factors we investigated did not show relevant modifications in overweight serum samples compared with controls.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Di Bernardo
authors.forename: Giovanni
authors.initials: G
authors.lastname: Messina
authors.forename: Stefania
authors.initials: S
authors.lastname: Capasso
authors.forename: Stefania
authors.initials: S
authors.lastname: Del Gaudio
authors.forename: Marilena
authors.initials: M
authors.lastname: Cipollaro
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Peluso
authors.forename: Fiorina
authors.initials: F
authors.lastname: Casale
authors.forename: Marcellino
authors.initials: M
authors.lastname: Monda
authors.forename: Umberto
authors.initials: U
authors.lastname: Galderisi
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects; D050156:Adipogenesis; D000293:Adolescent; D000328:Adult; D001798:Blood Proteins / Q000494:pharmacology; D016022:Case-Control Studies; D049109:Cell Proliferation; D002478:Cells, Cultured; D002648:Child; D016207:Cytokines / Q000097:blood / Q000494:pharmacology; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D009765:Obesity / Q000097:blood*; D010012:Osteogenesis; D017382:Reactive Oxygen Species / Q000097:blood / Q000494:pharmacology; D044967:Serum / Q000737:chemistry*
chemical_list: D001798:Blood Proteins; D016207:Cytokines; D017382:Reactive Oxygen Species