title: Evidence of sulfur-bound reduced copper in bamboo exposed to high silicon and copper concentrations.
abstract: We examined copper (Cu) absorption, distribution and toxicity and the role of a silicon (Si) supplementation in the bamboo Phyllostachys fastuosa. Bamboos were maintained in hydroponics for 4 months and submitted to two different Cu (1.5 and 100 μm Cu(2+)) and Si (0 and 1.1 mM) concentrations. Cu and Si partitioning and Cu speciation were investigated by chemical analysis, microscopic and spectroscopic techniques. Copper was present as Cu(I) and Cu(II) depending on plant parts. Bamboo mainly coped with high Cu exposure by: (i) high Cu sequestration in the root (ii) Cu(II) binding to amino and carboxyl ligands in roots, and (iii) Cu(I) complexation with both organic and inorganic sulfur ligands in stems and leaves. Silicon supplementation decreased the visible damage induced by high Cu exposure and modified Cu speciation in the leaves where a higher proportion of Cu was present as inorganic Cu(I)S compounds, which may be less toxic.
authors.affiliation: CEREGE, CNRS, Aix-Marseille Univ., Europôle méditerranéen de l'Arbois, BP 80, 13545 Aix en Provence, France; Département de recherche développement de la société PHYTOREM S.A., Chemin de l'Autodrome, 13140 Miramas, France. Electronic address: collin.blanche@gmail.com.
authors.forename: Blanche
authors.initials: B
authors.lastname: Collin
authors.affiliation: CIRAD, UPR Recyclage et risque, Avenue Agropolis, F-34398 Montpellier, France. Electronic address: doelsch@cirad.fr.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Doelsch
authors.affiliation: CEREGE, CNRS, Aix-Marseille Univ., Europôle méditerranéen de l'Arbois, BP 80, 13545 Aix en Provence, France. Electronic address: keller@cerege.fr.
authors.forename: Catherine
authors.initials: C
authors.lastname: Keller
authors.affiliation: CIRAD, UPR Recyclage et risque, Avenue Agropolis, F-34398 Montpellier, France. Electronic address: Patrick.cazevieille@cirad.fr.
authors.forename: Patrick
authors.initials: P
authors.lastname: Cazevieille
authors.affiliation: CIRAD, UPR Recyclage et risque, Avenue Agropolis, F-34398 Montpellier, France. Electronic address: marie.tella@cirad.fr.
authors.forename: Marie
authors.initials: M
authors.lastname: Tella
authors.affiliation: CEREGE, CNRS, Aix-Marseille Univ., Europôle méditerranéen de l'Arbois, BP 80, 13545 Aix en Provence, France. Electronic address: chaurand@cerege.fr.
authors.forename: Perrine
authors.initials: P
authors.lastname: Chaurand
authors.affiliation: Département de recherche développement de la société PHYTOREM S.A., Chemin de l'Autodrome, 13140 Miramas, France. Electronic address: fredericpanfili@phytorem.com.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Panfili
authors.affiliation: Institut Néel, CNRS and Université Joseph Fourier, BP 166, F-38042 Grenoble Cedex 9, France. Electronic address: jean-louis.hazemann@grenoble.cnrs.fr.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Hazemann
authors.affiliation: CEREGE, CNRS, Aix-Marseille Univ., Europôle méditerranéen de l'Arbois, BP 80, 13545 Aix en Provence, France. Electronic address: meunier@cerege.fr.
authors.forename: Jean-Dominique
authors.initials: JD
authors.lastname: Meunier
mesh_terms: D003300:Copper / Q000032:analysis* / Q000378:metabolism; D018527:Hydroponics; D008956:Models, Chemical; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D006109:Poaceae / Q000737:chemistry / Q000502:physiology*; D012825:Silicon / Q000032:analysis* / Q000378:metabolism; D013455:Sulfur / Q000032:analysis* / Q000378:metabolism
chemical_list: D013455:Sulfur; D003300:Copper; D012825:Silicon
keywords: Metal; Phytoremediation; Poaceae; Toxicity; X-ray absorption spectroscopy