title: A promiscuous prenyltransferase from Aspergillus oryzae catalyses C-prenylations of hydroxynaphthalenes in the presence of different prenyl donors.
abstract: Prenyltransferases of the dimethylallyltryptophan synthase (DMATS) superfamily are involved in the biosynthesis of secondary metabolites and show broad substrate specificity towards their aromatic substrates with a high regioselectivity for the prenylation reactions. Most members of this superfamily accepted as prenyl donor exclusively dimethylallyl diphosphate (DMAPP). One enzyme, AnaPT from Neosartorya fischeri, was reported recently to use both DMAPP and geranyl diphosphate (GPP) as prenyl donors. In this study, we demonstrate the acceptance of DMAPP, GPP and farnesyl diphosphate (FPP) by a new member of this superfamily, BAE61387 from Aspergillus oryzae DSM1147, for C-prenylations of hydroxynaphthalenes.
authors.affiliation: Institut für Pharmazeutische Biologie und Biotechnologie, Philipps-Universität Marburg, Deutschhausstrasse 17A, 35037, Marburg, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Pockrandt
authors.forename: Christopher
authors.initials: C
authors.lastname: Sack
authors.forename: Tatjana
authors.initials: T
authors.lastname: Kosiol
authors.forename: Shu-Ming
authors.initials: SM
authors.lastname: Li
mesh_terms: D001236:Aspergillus oryzae / Q000201:enzymology*; D004122:Dimethylallyltranstransferase / Q000378:metabolism*; D011756:Diphosphates / Q000378:metabolism*; D004224:Diterpenes / Q000378:metabolism*; D045782:Hemiterpenes / Q000378:metabolism*; D009284:Naphthols / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000378:metabolism*; D011106:Polyisoprenyl Phosphates / Q000378:metabolism*; D054876:Prenylation; D012717:Sesquiterpenes / Q000378:metabolism*
chemical_list: D011756:Diphosphates; D004224:Diterpenes; D045782:Hemiterpenes; D009284:Naphthols; D009943:Organophosphorus Compounds; D011106:Polyisoprenyl Phosphates; D012717:Sesquiterpenes; C511282:geranyl diphosphate; C043060:3,3-dimethylallyl pyrophosphate; C004808:farnesyl pyrophosphate; D004122:Dimethylallyltranstransferase