title: Hydroxyapatite-silver nanoparticles coatings on porous polyurethane scaffold.
abstract: The present paper is focused on a study regarding the possibility of obtaining hydroxyapatite-silver nanoparticle coatings on porous polyurethane scaffold. The method applied is based on a combined strategy involving hydroxyapatite biomimetic deposition on polyurethane surface using a Supersaturated Calcification Solution (SCS), combined with silver ions reduction and in-situ crystallization processes on hydroxyapatite-polyurethane surface by sample immersing in AgNO3 solution. The morphology, composition and phase structure of the prepared samples were characterized by scanning electron microscopy coupled with energy dispersive X-ray spectroscopy (SEM-EDX), X-ray diffraction (XRD), UV-Vis spectroscopy and X-ray photoelectron spectroscopy (XPS) measurements. The data obtained show that a layer of hydroxyapatite was deposited on porous polyurethane support and the silver nanoparticles (average size 34.71 nm) were dispersed among and even on the hydroxyapatite crystals. Hydroxyapatite/polyurethane surface acts as a reducer and a stabilizing agent for silver ions. The surface plasmon resonance peak in UV-Vis absorption spectra showed an absorption maximum at 415 nm, indicating formation of silver nanoparticles. The hydroxyapatite-silver polyurethane scaffolds were tested against Staphylococcus aureus and Escherichia coli and the obtained data were indicative of good antibacterial properties of the materials.
authors.affiliation: "Gheorghe Asachi" Technical University of Iasi, Faculty of Chemical Engineering and Environmental Protection, Prof. dr. docent Dimitrie Mangeron Rd., no. 63, 700050, Iasi, Romania. Electronic address: gciobanu03@yahoo.co.uk.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Ciobanu
authors.affiliation: "Gheorghe Asachi" Technical University of Iasi, Faculty of Chemical Engineering and Environmental Protection, Prof. dr. docent Dimitrie Mangeron Rd., no. 63, 700050, Iasi, Romania.
authors.forename: Simona
authors.initials: S
authors.lastname: Ilisei
authors.affiliation: "Gheorghe Asachi" Technical University of Iasi, Faculty of Chemical Engineering and Environmental Protection, Prof. dr. docent Dimitrie Mangeron Rd., no. 63, 700050, Iasi, Romania.
authors.forename: Constantin
authors.initials: C
authors.lastname: Luca
mesh_terms: D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000494:pharmacology; D018407:Bacterial Physiological Phenomena / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D017886:Durapatite / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011140:Polyurethanes / Q000737:chemistry; D016062:Porosity; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology*; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D011140:Polyurethanes; D012834:Silver; D017886:Durapatite
keywords: Coating; Hydroxyapatite; Polyurethane; Silver nanoparticles