title: SERS-based direct and sandwich assay methods for mir-21 detection.
abstract: In this study, two different assay methods were developed using a surface enhanced Raman scattering (SERS) label for sensitive miR-21 detection. In the first method (direct assay), the miR-21 probe was attached to SERS-labelled, rod-shaped gold nanoparticles and hybridised with the target miR-21, which was previously immobilised onto the gold slide. In the second method (sandwich assay), the target miR-21 was captured by an miR-21 probe immobilised onto the gold slide and hybridised with a second miR-21 probe immobilised on the SERS-labeled, rod-shaped gold nanoparticles. SERS signals of developed assays were obtained via a SERS spectrum of 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) on the rod-shaped nanoparticles. The calibration curves were plotted to measure the different concentrations of miR-21. The detection limits of the direct and sandwich assays, which last less than 40 min, were found to be 0.36 and 0.85 nM, respectively. The developed SERS-based methods offer rapid, selective, sensitive and easy detection of miR-21, especially compared to conventional PCR-based methods.
authors.affiliation: Department of Food Engineering, Faculty of Engineering, Hacettepe University, Beytepe, 06800 Ankara, Turkey. ihb@hacettepe.edu.tr.
authors.forename: Burcu
authors.initials: B
authors.lastname: Guven
authors.forename: Fahriye Ceyda
authors.initials: FC
authors.lastname: Dudak
authors.forename: Ismail Hakki
authors.initials: IH
authors.lastname: Boyaci
authors.forename: Ugur
authors.initials: U
authors.lastname: Tamer
authors.forename: Mehmet
authors.initials: M
authors.lastname: Ozsoz
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D035683:MicroRNAs / Q000032:analysis*; D009841:Oligonucleotides / Q000032:analysis; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: C531950:MIRN21 microRNA, mouse; D035683:MicroRNAs; D009841:Oligonucleotides; D006046:Gold