title: Glycerol as substrate for biomass and β-carotene production byRhodotorula lactosa.
abstract: Glycerol was used as the sole carbon and energy source for growingRhodotorula lactosa. The maximum biomass yield (0.53 g/g substrate) was obtained after 20 h with 21.5 g glycerol/l; growth was inhibited with 28.0 g glycerol/l and cell morphology was changed. At this time, the cells were not pigmented. After 48 h of cultivation, β-carotene was at 1.8 mg/g dry cells, yielding 22.0 mg/l. When cells were grown for 20 h, washed, suspended in distilled water and aerated for 24 hours, more β-carotene (2.66 mg/g dry cells or 28.0 mg/l of the original culture) was produced. Cell protein content after 48 h was 36 to 38% (w/w) before extraction and 45 to 47% (w/w) for acetone-extracted cells.
authors.affiliation: Departamento de Engenharia Bioquimica, Escola de Quimica, Universidade Federal do Rio de Janeiro, 2194, Rio de Janeiro, Brazil.
authors.forename: H L
authors.initials: HL
authors.lastname: Martelli
authors.forename: I M
authors.initials: IM
authors.lastname: da Silva
authors.forename: N O
authors.initials: NO
authors.lastname: Souza
authors.forename: D
authors.initials: D
authors.lastname: Pomeroy