title: Mutants of phospholipase A (pPLA-I) have a red light and auxin phenotype.
abstract: pPLA-I is the evolutionarily oldest patatin-related phospholipase A (pPLA) in plants, which have previously been implicated to function in auxin and defence signalling. Molecular and physiological analysis of two allelic null mutants for pPLA-I [ppla-I-1 in Wassilewskija (Ws) and ppla-I-3 in Columbia (Col) ] revealed pPLA-I functions in auxin and light signalling. The enzyme is localized in the cytosol and to membranes. After auxin application expression of early auxin-induced genes is significantly slower compared with wild type and both alleles show a slower gravitropic response of hypocotyls, indicating compromised auxin signalling. Additionally, phytochrome-modulated responses like abrogation of gravitropism, enhancement of phototropism and growth in far red-enriched light are decreased in both alleles. While early flowering, root coils and delayed phototropism are only observed in the Ws mutant devoid of phyD, the light-related phenotypes observed in both alleles point to an involvement of pPLA-I in phytochrome signalling.
authors.affiliation: Leibniz Universität Hannover, Institut für Zierpflanzenbau und Gehölzwissenschaften, Abt. Molekulare Ertragsphysiologie, D-30419, Hannover, Germany.
authors.forename: Yunus
authors.initials: Y
authors.lastname: Effendi
authors.forename: Katrin
authors.initials: K
authors.lastname: Radatz
authors.forename: Corinna
authors.initials: C
authors.lastname: Labusch
authors.forename: Steffen
authors.initials: S
authors.lastname: Rietz
authors.forename: Rinukshi
authors.initials: R
authors.lastname: Wimalasekera
authors.forename: Hanna
authors.initials: H
authors.lastname: Helizon
authors.forename: Mathias
authors.initials: M
authors.lastname: Zeidler
authors.forename: Günther F E
authors.initials: GF
authors.lastname: Scherer
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000235:genetics* / Q000378:metabolism; D005091:Exons / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018522:Gravitropism / Q000187:drug effects; D018546:Hypocotyl / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D007210:Indoleacetic Acids / Q000494:pharmacology*; D007438:Introns / Q000235:genetics; D008027:Light; D009154:Mutation / Q000235:genetics*; D010641:Phenotype; D010741:Phospholipases A / Q000235:genetics* / Q000378:metabolism; D018524:Phototropism / Q000187:drug effects; D051939:Phytochrome B / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000502:physiology; D021381:Protein Transport / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D012333:RNA, Messenger; D051939:Phytochrome B; D002265:Carboxylic Ester Hydrolases; C522182:PLAI protein, Arabidopsis; D010741:Phospholipases A
keywords: Arabidopsis; gravitropism; patatin-related phospholipase A; phototropism; phytochrome; root coiling; shade avoidance