title: Effect of zearalenone on reproductive parameters and expression of selected testicular genes in mice.
abstract: We tested the effect of two different concentrations (150μg/l and 0.15μg/l) of mycotoxin zearalenone (ZEA) on the reproductive parameters and expression of testicular genes in male mice. In adult males, no reduction of body or reproductive organ weight was observed, and the seminiferous tubules were morphologically normal with ongoing spermatogenesis. However, we found decreased sperm concentration, increase of morphologically abnormal spermatozoa and increased binding of apoptotic marker annexin V. This study was also focused on the evaluation of gene expression profiles of 28 genes playing important roles during the processes occurring in the testicular tissue. We detected changes in the expression of genes important for proper spermatogenesis. Surprisingly, we observed a stronger effect after exposure to the lower dose of ZEA.
authors.affiliation: Laboratory of Reproductive Biology, Institute of Biotechnology, Academy of Sciences of the Czech Republic, v. v. i., Prague, Czech Republic.
authors.forename: E
authors.initials: E
authors.lastname: Zatecka
authors.affiliation: Laboratory of Reproductive Biology, Institute of Biotechnology, Academy of Sciences of the Czech Republic, v. v. i., Prague, Czech Republic.
authors.forename: L
authors.initials: L
authors.lastname: Ded
authors.affiliation: Laboratory of Reproductive Biology, Institute of Biotechnology, Academy of Sciences of the Czech Republic, v. v. i., Prague, Czech Republic.
authors.forename: F
authors.initials: F
authors.lastname: Elzeinova
authors.affiliation: Laboratory of Reproductive Biology, Institute of Biotechnology, Academy of Sciences of the Czech Republic, v. v. i., Prague, Czech Republic.
authors.forename: A
authors.initials: A
authors.lastname: Kubatova
authors.affiliation: Laboratory of Reproductive Biology, Institute of Biotechnology, Academy of Sciences of the Czech Republic, v. v. i., Prague, Czech Republic.
authors.forename: A
authors.initials: A
authors.lastname: Dorosh
authors.affiliation: Laboratory of Reproductive Biology, Institute of Biotechnology, Academy of Sciences of the Czech Republic, v. v. i., Prague, Czech Republic.
authors.forename: H
authors.initials: H
authors.lastname: Margaryan
authors.affiliation: Laboratory of Reproductive Biology, Institute of Biotechnology, Academy of Sciences of the Czech Republic, v. v. i., Prague, Czech Republic.
authors.forename: P
authors.initials: P
authors.lastname: Dostalova
authors.affiliation: Laboratory of Gene Expression, Institute of Biotechnology, Academy of Sciences of the Czech Republic, v. v. i., Prague, Czech Republic.
authors.forename: V
authors.initials: V
authors.lastname: Korenkova
authors.affiliation: Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, Prague, Czech Republic.
authors.forename: K
authors.initials: K
authors.lastname: Hoskova
authors.affiliation: Laboratory of Reproductive Biology, Institute of Biotechnology, Academy of Sciences of the Czech Republic, v. v. i., Prague, Czech Republic. Electronic address: jana.peknicova@img.cas.cz.
authors.forename: J
authors.initials: J
authors.lastname: Peknicova
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D004968:Estrogens, Non-Steroidal / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects; D008297:Male; D051379:Mice; D013091:Spermatogenesis / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000473:pathology; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D015025:Zearalenone / Q000633:toxicity*
chemical_list: D004968:Estrogens, Non-Steroidal; D015025:Zearalenone
keywords: Fertility; Gene expression; Reproductive parameters in male mice; Spermatogenesis; Zearalenone; qPCR