title: Production and regeneration of protoplasts from the mycorrhizal fungus Suillus granulatus.
abstract: Experiments were performed with the mycorrhizal fungus Suillus granulatus to define the parameters for production and regeneration of protoplasts. Protoplasts were released at frequencies between 1 and 3×10(7)/ml from mycelium 3 to 7 days old. The best osmotic stabilizer for protoplast release was MgSO4 (0.7 M). To optimize protoplast release and regeneration an enzyme (Novozym 234) concentration 1.7 mg/ml was chosen, with a digestion time of 1 to 2 h. Regenerated colonies formed mycorrhizae within 60 days after inoculation in Pinus caribaea var. hondurensis seedlings.
authors.affiliation: Departamento de Microbiologia, Universidade Federal de Viçosa, Viçosa 36570-000, Minas Gerais, Brasil.
authors.forename: E S
authors.initials: ES
authors.lastname: Dias
authors.forename: E F
authors.initials: EF
authors.lastname: Araújo
authors.forename: W V
authors.initials: WV
authors.lastname: Guimarães
authors.forename: R M
authors.initials: RM
authors.lastname: Muchovej