title: Organic selenium supplementation increases PHGPx but does not improve viability in chilled boar semen.
abstract: This study evaluated the effects of dietary organic selenium (Se) on viability of chilled boar semen. Twelve boars were divided into three groups: control (CON), 0.3 mg kg(-1) sodium selenite; inorganic (INO), 0.5 mg kg(-1) sodium selenite and organic (ORG), 0.5 mg kg(-1) Se yeast. The experiment was conducted within 10 weeks, and analysis was performed fortnightly, in storage semen by 72 h. No effect was observed on motility; however, straightness and linearity percentages were higher (P < 0.05) in the animals receiving CON diet compared with INO group. Percentages of cells with both plasma and acrosomal intact membranes, lipidic membrane peroxidation and mitochondrial membrane potential were similar on all treatments. Animals receiving CON diet presented higher (P < 0.05) values of ATP when compared with INO group. The PHGPx was higher (P < 0.05) in animals that received ORG in comparison with INO group. In conclusion, organic selenium supplementation increases PHGPx but does not improve chilled semen viability in 72 h.
authors.affiliation: Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo (USP), Sao Paulo, Brazil; Department of Animal Nutrition and Production, School of Veterinary Medicine and Animal Science, University of Sao Paulo (USP), Pirassununga, Brazil.
authors.forename: S M M K
authors.initials: SM
authors.lastname: Martins
authors.forename: A F C
authors.initials: AF
authors.lastname: De Andrade
authors.forename: F G
authors.initials: FG
authors.lastname: Zaffalon
authors.forename: F F
authors.initials: FF
authors.lastname: Bressan
authors.forename: S M P
authors.initials: SM
authors.lastname: Pugine
authors.forename: M P
authors.initials: MP
authors.lastname: Melo
authors.forename: M R
authors.initials: MR
authors.lastname: Chiaratti
authors.forename: C T
authors.initials: CT
authors.lastname: Marino
authors.forename: A S
authors.initials: AS
authors.lastname: Moretti
authors.forename: R P
authors.initials: RP
authors.lastname: Arruda
mesh_terms: D000177:Acrosome / Q000187:drug effects; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D019587:Dietary Supplements; D005979:Glutathione Peroxidase / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D012643:Selenium / Q000494:pharmacology*; D055101:Semen Analysis; D012662:Semen Preservation; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000201:enzymology; D013552:Swine
chemical_list: D000975:Antioxidants; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D005979:Glutathione Peroxidase; D012643:Selenium
keywords: Chilled boar semen; PHGPx; organic selenium; sperm motility