title: Deep sequencing of the tobacco mitochondrial transcriptome reveals expressed ORFs and numerous editing sites outside coding regions.
abstract: BACKGROUND
  The purpose of this study was to sequence and assemble the tobacco mitochondrial transcriptome and obtain a genomic-level view of steady-state RNA abundance. Plant mitochondrial genomes have a small number of protein coding genes with large and variably sized intergenic spaces. In the tobacco mitogenome these intergenic spaces contain numerous open reading frames (ORFs) with no clear function.
  
  
  RESULTS
  The assembled transcriptome revealed distinct monocistronic and polycistronic transcripts along with large intergenic spaces with little to no detectable RNA. Eighteen of the 117 ORFs were found to have steady-state RNA amounts above background in both deep-sequencing and qRT-PCR experiments and ten of those were found to be polysome associated. In addition, the assembled transcriptome enabled a full mitogenome screen of RNA C→U editing sites. Six hundred and thirty five potential edits were found with 557 occurring within protein-coding genes, five in tRNA genes, and 73 in non-coding regions. These sites were found in every protein-coding transcript in the tobacco mitogenome.
  
  
  CONCLUSIONS
  These results suggest that a small number of the ORFs within the tobacco mitogenome may produce functional proteins and that RNA editing occurs in coding and non-coding regions of mitochondrial transcripts.
authors.forename: Benjamin T
authors.initials: BT
authors.lastname: Grimes
authors.forename: Awa K
authors.initials: AK
authors.lastname: Sisay
authors.forename: Hyrum D
authors.initials: HD
authors.lastname: Carroll
authors.affiliation: Department of Biology, Box 60, Middle Tennessee State University, Murfreesboro, TN 37132, USA. Aubrey.Cahoon@mtsu.edu.
authors.forename: A Bruce
authors.initials: AB
authors.lastname: Cahoon
mesh_terms: D050259:Genes, Mitochondrial; D059014:High-Throughput Nucleotide Sequencing; D008928:Mitochondria / Q000235:genetics* / Q000378:metabolism; D016366:Open Reading Frames / Q000235:genetics*; D011132:Polyribosomes / Q000378:metabolism; D017393:RNA Editing; D012335:RNA, Ribosomal / Q000302:isolation & purification / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics*; D059467:Transcriptome
chemical_list: D012335:RNA, Ribosomal; D012343:RNA, Transfer