title: [Effect of electroacupuncture on phosphorylation of NR2B at Tyr 1742 site in the spinal dorsal horn of CFA rats].
abstract: OBJECTIVE
  To observe the effect of electroacupuncture (EA) on phosphorylation of spinal NR2B at Tyr 1742 site in complete Freund's adjuvant (CFA) induced inflammatory pain rats. METHods Forty male Sprague Dawley rats were randomly divided into normal group (N group, n = 10), the model group (CFA group, n = 15), and the EA group (n = 15). The inflammatory pain model was established by subcutaneous injecting CFA (0.1 mL per rat) into the right hind paw. Paw withdrawal thresholds (PWTs) were measured before CFA injection (as the base), as well as at 24 h, 25 h, 3rd day, and 7th day after CFA injection. Phosphorylation of NR2B at Tyr 1742 site in the ispilateral spinal dorsal horn at the 3rd day post-injection were detected using immunohistochemical assay.
  
  
  RESULTS
  PWTs in the CFA group were significantly lower than those of the N group at every detective time point post-injection (P < 0.01). PWTs were obviously lower in the EA group than in the N group at 24 h post-injection (P < 0.01). It showed increasing tendency, markedly higher than those of the CFA group at 25 h and 3rd day post-injection (P < 0.01). Compared with the N group, the ratio of p-NR2B positive cells in the ispilateral spinal dorsal horn of rats in the CFA group was up-regulated. Compared with the CFA group, the ratio of p-NR2B positive cells in the ispilateral spinal dorsal horn of rats showed a decreasing tendency in the EA group.
  
  
  CONCLUSIONS
  EA might effectively inhibit CFA-induced inflammatory pain possibly associated with down-regulating phosphorylation of NR2B at Tyr 1742 site in the ispilateral spinal dorsal horn.
authors.affiliation: Department of Neurobiology and Acupuncture Research, Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Department of Neurobiology and Acupuncture Research, Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors.forename: Jian-Qiao
authors.initials: JQ
authors.lastname: Fang
authors.affiliation: Department of Neurobiology and Acupuncture Research, Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors.forename: Jun-Fan
authors.initials: JF
authors.lastname: Fang
authors.affiliation: Department of Neurobiology and Acupuncture Research, Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors.forename: Jun-Ying
authors.initials: JY
authors.lastname: Du
authors.affiliation: Department of Neurobiology and Acupuncture Research, Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Qiu
authors.affiliation: Department of Neurobiology and Acupuncture Research, Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
mesh_terms: D000277:Adjuvants, Pharmaceutic / Q000494:pharmacology; D000818:Animals; D015671:Electroacupuncture / Q000379:methods; D008297:Male; D010146:Pain / Q000139:chemically induced / Q000378:metabolism*; D010766:Phosphorylation; D020671:Posterior Horn Cells / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*
chemical_list: D000277:Adjuvants, Pharmaceutic; C121001:NR2B NMDA receptor; D016194:Receptors, N-Methyl-D-Aspartate