title: Dynamic changes in cell-surface expression of mannose in the oral epithelium during the development of graft-versus-host disease of the oral mucosa in rats.
abstract: BACKGROUND
  The role of cell-surface glycoconjugates in oral mucosal graft-versus-host disease (GVHD) is still unclear, even though molecular changes in the oral epithelium are essential for the pathogenesis of these lesions. In this study, we investigated changes in the binding of mannose (Man)-specific Lens culinaris lectin (LCA) in the oral mucosa of rats with GVHD.
  
  
  METHODS
  Lewis rat spleen cells were injected into (Lewis x Brown Norway) F1 rats to induce systemic GVHD, including oral mucosal lesions. Tongue and spleen samples were evaluated using lectin histochemistry, immunohistochemistry, Western blotting, transwell migration assays and Stamper-Woodruff binding assays.
  
  
  RESULTS
  Binding of Man-specific LCA expanded to the epithelial layers of the tongue in GVHD-rats. An expansion of LCA binding was related to the increased expression of mannosyltransferase in the oral mucosa. CD8+ cells, effector cells of oral mucosal GVHD, expressed mannose-binding protein (MBP) and migrated to the medium containing Man in the transwell migration assay. Adherence of CD8+ cells to the oral epithelium could be inhibited by pretreating CD8+ cells with MBP antibody and/or by pretreating sections with Man-specific LCA.
  
  
  CONCLUSIONS
  Increased expression of Man on keratinocytes leads to the migration and/or adhesion of CD8+ cells in the surface epithelium, which is mediated in part by the MBP/Man-binding pathway during the development of oral mucosal GVHD.
authors.forename: Hironori
authors.initials: H
authors.lastname: Hanada
authors.affiliation: Department of Morphological Biology, Division of Pathology, Fukuoka Dental College, Tamura, Fukuoka 2-15-1, Japan. johno@college.fdcnet.ac.jp.
authors.forename: Jun
authors.initials: J
authors.lastname: Ohno
authors.forename: Kei
authors.initials: K
authors.lastname: Seno
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Ota
authors.forename: Kunihisa
authors.initials: K
authors.lastname: Taniguchi
mesh_terms: D000818:Animals; D015153:Blotting, Western; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism / Q000473:pathology; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D017690:Cell Transplantation / Q000379:methods; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D005260:Female; D006086:Graft vs Host Disease / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000378:metabolism / Q000473:pathology; D027806:Lens Plant; D008358:Mannose / Q000378:metabolism*; D037601:Mannose-Binding Lectin / Q000378:metabolism; D008364:Mannosyltransferases / Q000032:analysis; D009059:Mouth Diseases / Q000378:metabolism* / Q000473:pathology; D009061:Mouth Mucosa / Q000378:metabolism* / Q000473:pathology; D037121:Plant Lectins / Q000494:pharmacology; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D013154:Spleen / Q000166:cytology; D014060:Tongue Diseases / Q000378:metabolism / Q000473:pathology
chemical_list: D037601:Mannose-Binding Lectin; D037121:Plant Lectins; C033619:lentil lectin; D008364:Mannosyltransferases; D008358:Mannose