title: Border forces and friction control epithelial closure dynamics.
abstract: We study the closure dynamics of a large number of well-controlled circular apertures within an epithelial monolayer, where the collective cell migration responsible for epithelization is triggered by the removal of a spatial constraint rather than by scratching. Based on experimental observations, we propose a physical model that takes into account border forces, friction with the substrate, and tissue rheology. Border protrusive activity drives epithelization despite the presence of a contractile actomyosin cable at the periphery of the wound. The closure dynamics is quantified by an epithelization coefficient, defined as the ratio of protrusive stress to tissue-substrate friction, that allows classification of different phenotypes. The same analysis demonstrates a distinct signature for human cells bearing the oncogenic RasV12 mutation, demonstrating the potential of the approach to quantitatively characterize metastatic transformations.
authors.affiliation: Physico-Chimie Curie, Unité Mixte de Recherche 168, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, Paris, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Cochet-Escartin
authors.affiliation: Physico-Chimie Curie, Unité Mixte de Recherche 168, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, Paris, France.
authors.forename: Jonas
authors.initials: J
authors.lastname: Ranft
authors.affiliation: Physico-Chimie Curie, Unité Mixte de Recherche 168, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, Paris, France. Electronic address: pascal.silberzan@curie.fr.
authors.forename: Pascal
authors.initials: P
authors.lastname: Silberzan
authors.affiliation: Physico-Chimie Curie, Unité Mixte de Recherche 168, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, Paris, France. Electronic address: philippe.marcq@curie.fr.
authors.forename: Philippe
authors.initials: P
authors.lastname: Marcq
mesh_terms: D000205:Actomyosin / Q000378:metabolism; D000818:Animals; D002465:Cell Movement; D004285:Dogs; D004847:Epithelial Cells / Q000378:metabolism / Q000502:physiology*; D017276:Friction; D057809:HEK293 Cells; D006801:Humans; D061985:Madin Darby Canine Kidney Cells; D008954:Models, Biological; D010641:Phenotype; D062326:Re-Epithelialization; D012212:Rheology
chemical_list: D000205:Actomyosin