title: Anti-inflammatory and antiapoptotic effect of interleukine-18 binding protein on the spinal cord ischemia-reperfusion injury.
abstract: We investigated the anti-inflammatory and antiapoptotic effects of interleukin-18 binding protein (IL-18BP) on spinal cord ischemia/reperfusion (I/R) injury in rats. Twenty-one adult male rats were divided into three groups: sham, I/R, and I/R+IL-18BP. Proinflammatory cytokines were determined in rat blood samples by using ELISA, while apoptosis was immunohistochemically evaluated in spinal cord tissue using caspase 3. Both IL-18 and TNF-α were significantly decreased in the IL-18BP group compared to that in the sham group. The highest caspase 3 levels were observed in the I/R group, while the lowest levels were found in the sham group. The mean Tarlov score of the I/R group was significantly lower than that of the sham group. However, the mean Tarlov score of the IL-18BP group was significantly higher than that of the I/R group. The results of the current study demonstrate that IL-18BP plays both anti-inflammatory and antiapoptotic roles in spinal cord I/R injury.
authors.affiliation: Department of Neurosurgery, Faculty of Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey.
authors.forename: Ergün
authors.initials: E
authors.lastname: Karavelioğlu
authors.forename: Yücel
authors.initials: Y
authors.lastname: Gönül
authors.forename: Serdar
authors.initials: S
authors.lastname: Kokulu
authors.forename: Ömer
authors.initials: Ö
authors.lastname: Hazman
authors.forename: Fatih
authors.initials: F
authors.lastname: Bozkurt
authors.forename: Ahmet
authors.initials: A
authors.lastname: Koçak
authors.forename: Olcay
authors.initials: O
authors.lastname: Eser
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D051017:Apoptosis Regulatory Proteins / Q000494:pharmacology*; D053148:Caspase 3 / Q000378:metabolism; D016207:Cytokines / Q000097:blood; D004195:Disease Models, Animal; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D020382:Interleukin-18 / Q000097:blood; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy*; D013116:Spinal Cord; D020760:Spinal Cord Ischemia / Q000188:drug therapy*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D000893:Anti-Inflammatory Agents; D051017:Apoptosis Regulatory Proteins; D016207:Cytokines; D036341:Intercellular Signaling Peptides and Proteins; D020382:Interleukin-18; D014409:Tumor Necrosis Factor-alpha; C118370:interleukin-18 binding protein; D053148:Caspase 3