title: Pregnancy rate and birth rate of calves from a large-scale IVF program using reverse-sorted semen in Bos indicus, Bos indicus-taurus, and Bos taurus cattle.
abstract: Obtaining sexed sperm from previously frozen doses (reverse-sorted semen [RSS]) provides an important advantage because of the possibility of using the semen of bulls with desired genetic attributes that have died or have become infertile but from whom frozen semen is available. We report the efficiency of RSS on the pregnancy rate and birth rate of calves in a large-scale program using ovum pick-up and in vitro embryo production (IVEP) from Bos indicus, Bos indicus-taurus, and Bos taurus cattle. From 645 ovum pick-up procedures (Holstein, Gir, and Nelore), 9438 viable oocytes were recovered. A dose of frozen semen (Holstein, Nelore, Brahman, Gir, and Braford) was thawed, and the sperm were sex-sorted and cooled for use in IVF. Additionally, IVF with sperm from three Holstein bulls with freeze-thawed, sex-sorted (RSS) or sex-sorted, freeze-thawed (control) was tested. A total of 2729 embryos were produced, exhibiting a mean blastocyst rate of 29%. Heifers and cows selected for adequate body condition, estrus, and health received 2404 embryos, and 60 days later, a 41% average pregnancy rate was observed. A total of 966 calves were born, and 910 were of a predetermined sex, with an average of 94% accuracy in determining the sex. Despite the lower blastocyst rate with freeze-thawed, sex-sorted semen compared with sex-sorted semen, (P < 0.05), the pregnancy rate (bull I, 45% vs. 40%; II, 35% vs. 50%; and III, 47% vs. 48% for RSS and control, respectively; P > 0.05) and sex-sorted efficiency (bull I, 93% vs. 98%; II, 96% vs. 94%; and III, 96% vs. 97% for RSS and control, respectively; P > 0.05) were similar for each of the three bulls regardless of the sperm type used in the IVF. The sexing of previously frozen semen, associated with IVEP, produces viable embryos with a pregnancy rate of up to 40%, and calves of the desired sex are born even if the paternal bull has acquired some infertility, died, or is located a long distance from the sexing laboratory. Furthermore, these data show the feasibility of the process even when used in a large-scale IVEP program.
authors.affiliation: Laboratório de Reprodução Animal, DCV-CCA-Universidade Estadual de Londrina (UEL), Londrina, Parana, Brazil.
authors.forename: F
authors.initials: F
authors.lastname: Morotti
authors.affiliation: In Vitro Brasil Ltda, Mogi Mirim, Sao Paulo, Brazil.
authors.forename: B V
authors.initials: BV
authors.lastname: Sanches
authors.affiliation: In Vitro Brasil Ltda, Mogi Mirim, Sao Paulo, Brazil.
authors.forename: J H F
authors.initials: JH
authors.lastname: Pontes
authors.affiliation: In Vitro Brasil Ltda, Mogi Mirim, Sao Paulo, Brazil.
authors.forename: A C
authors.initials: AC
authors.lastname: Basso
authors.affiliation: In Vitro Brasil Ltda, Mogi Mirim, Sao Paulo, Brazil.
authors.forename: E R
authors.initials: ER
authors.lastname: Siqueira
authors.affiliation: Laboratório de Reprodução Animal, DCV-CCA-Universidade Estadual de Londrina (UEL), Londrina, Parana, Brazil.
authors.forename: L A
authors.initials: LA
authors.lastname: Lisboa
authors.affiliation: Laboratório de Reprodução Animal, DCV-CCA-Universidade Estadual de Londrina (UEL), Londrina, Parana, Brazil. Electronic address: mseneda@uel.br.
authors.forename: M M
authors.initials: MM
authors.lastname: Seneda
mesh_terms: D000818:Animals; D001723:Birth Rate; D002417:Cattle; D002469:Cell Separation / Q000379:methods / Q000662:veterinary*; D015925:Cryopreservation / Q000662:veterinary; D004624:Embryo Transfer / Q000662:veterinary; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods / Q000662:veterinary*; D008297:Male; D011247:Pregnancy; D018873:Pregnancy Rate; D012662:Semen Preservation / Q000662:veterinary; D012743:Sex Preselection / Q000379:methods / Q000662:veterinary*; D013094:Spermatozoa / Q000166:cytology*
keywords: Embryo; IVF; Ovum pick-up; Reverse sorted; Sex-sorted