title: Molecular mechanism of (-)-epigallocatechin-3-gallate on balloon injury-induced neointimal formation and leptin expression.
abstract: Leptin contributes to the pathogenesis of vascular repair and cardiovascular events. This study evaluated the molecular mechanism of EGCG in balloon injury-induced leptin expression. According to immunohistochemical and confocal analyses, leptin expression was increased and the aortic lumen exhibited narrowing after balloon injury. EGCG treatment attenuated leptin expression and diminished neointimal formation. The in vitro study showed that angiotensin II (Ang II) induced the migration and proliferation of cultured vascular smooth muscle cells (VSMCs), whereas treatment with EGCG, leptin siRNA, and c-Jun siRNA inhibited the migration and proliferation of VSMCs significantly. The EMSA shows that balloon injury increased AP-1-binding activity, and EGCG and c-Jun siRNA inhibited the AP-1-binding activity. Western blot and real-time RT-PCR analyses revealed similar results in intimal tissue samples. In summary, balloon injury induces leptin expression in the carotid artery of rats, and EGCG inhibits leptin expression through the JNK/AP-1 pathway and also attenuates neointimal formation.
authors.affiliation: Department of Emergency Medicine, Shin Kong Wu Ho-Su Memorial Hospital , Taipei, Taiwan.
authors.forename: Chiu-Mei
authors.initials: CM
authors.lastname: Lin
authors.forename: Sheng-Wen
authors.initials: SW
authors.lastname: Hou
authors.forename: Bao-Wei
authors.initials: BW
authors.lastname: Wang
authors.forename: Jiann-Ruey
authors.initials: JR
authors.lastname: Ong
authors.forename: Hang
authors.initials: H
authors.lastname: Chang
authors.forename: Kou-Gi
authors.initials: KG
authors.lastname: Shyu
mesh_terms: D000800:Angioplasty, Balloon / Q000009:adverse effects*; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D001013:Aorta, Thoracic / Q000737:chemistry / Q000293:injuries / Q000473:pathology; D002339:Carotid Arteries / Q000737:chemistry; D020212:Carotid Artery Injuries / Q000503:physiopathology*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D020738:Leptin / Q000032:analysis* / Q000235:genetics; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D058426:Neointima / Q000517:prevention & control*; D034741:RNA, Small Interfering / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D020738:Leptin; D034741:RNA, Small Interfering; D000804:Angiotensin II; D002392:Catechin; C045651:epigallocatechin gallate