title: Intracranial transplantation of human adipose-derived stem cells promotes the expression of neurotrophic factors and nerve repair in rats of cerebral ischemia-reperfusion injury.
abstract: Adipose tissue-derived mesenchymal stem cells (ADSCs) are of great interest as a cellular therapeutic agent for regenerative and immunomodulatory purposes. The aim of this study was to investigate whether ADSCs transplantation could promote nerve repair in rats of cerebral ischemia-reperfusion (I/R) injury. We isolated and cultured human ADSCs, and then measured cell surface antigens by flow cytometry and immunofluorescence. Healthy SD rats were randomly divided into sham group, MCAO group, MCAO+vehicle group and MCAO+ADSCs group. Cerebral ischemia-reperfusion injury was induced by middle cerebral artery occlusion (MCAO). Then the human ADSCs were transplanted into the brain of rats 24 h after MCAO. The mRNA level of BDNF (brain derived neurotrophic factor, BDNF), NGF (nerve growth factor, NGF) and bFGF (basic fibroblasts growth factor, bFGF) were detected by real-time PCR at different time points (d7, d14, d21 and d28 after MCAO). Meanwhile, the neurological deficit scores were estimated. The neurological deficit of rats in MCAO+ADSCs group attenuated at d7 in contrast to the MCAO+vehicle group (P<0.05). Subsequently, they were dramatically ameliorated with the time especially at d28. At d7, d14, d21 and d28 after ADSCs transplantation, BDNF, NGF and bFGF mRNA in MCAO+ADSCs group were strikingly higher than those in MCAO+vehicle group, and these two groups both reached the peak at d14. The western blotting results showed that BDNF and Bcl-2 expressed higher in MCAO+ADSCs group than MCAO+vehicle group. Therefore, our current results suggest that ADSCs promote nerve repair after injury through elevating the expression of neurotrophic factors and inhibiting the apoptosis of neural cells.
authors.affiliation: Plastic Surgery Institute of Weifang Medical University Weifang, 261041, Shandong Province, China.
authors.forename: Xing-Long
authors.initials: XL
authors.lastname: Liu
authors.affiliation: Plastic Surgery Institute of Weifang Medical University Weifang, 261041, Shandong Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Plastic Surgery Institute of Weifang Medical University Weifang, 261041, Shandong Province, China.
authors.forename: Sheng-Jian
authors.initials: SJ
authors.lastname: Tang
mesh_terms: D017667:Adipocytes / Q000637:transplantation; D000293:Adolescent; D000328:Adult; D000818:Animals; D015153:Blotting, Western; D002545:Brain Ischemia / Q000378:metabolism; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D009414:Nerve Growth Factors / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D020127:Recovery of Function; D015427:Reperfusion Injury / Q000378:metabolism; D055815:Young Adult
chemical_list: D009414:Nerve Growth Factors
keywords: Adipose-derived stem cells; BDNF; NGF; bFGF; cerebral ischemia-reperfusion injury; nerve repair