title: Thermostable extracellular protease of Bacillus stearothermophilus: factors affecting its production.
abstract: A strain of protease-producing Bacillus stearothermophilus has been isolated. Glycerol was the best carbon source for production whereas yeast extract was the best nitrogen source. The bacterium could grow up to 70°C but optimum protease production was at 60°C. Best initial pH for protease production was 5. Alkaline pH inhibited production. The enzyme was stable at 60°C for 18 h and was inhibited by EDTA, PMSF and HgCl2.
authors.affiliation: , .
authors.forename: N A
authors.initials: NA
authors.lastname: Razak
authors.forename: M Y
authors.initials: MY
authors.lastname: Samad
authors.forename: M
authors.initials: M
authors.lastname: Basri
authors.forename: W M
authors.initials: WM
authors.lastname: Yunus
authors.forename: K
authors.initials: K
authors.lastname: Ampon
authors.forename: A B
authors.initials: AB
authors.lastname: Salleh