title: Pseudomonas putida response in membrane bioreactors under salicylic acid-induced stress conditions.
abstract: Starvation and changing feeding conditions are frequently characteristics of wastewater treatment plants. They are typical causes of unsteady-state operation of biological systems and provoke cellular stress. The response of a membrane bioreactor functioning under feed-induced stress conditions is studied here. In order to simplify and considerably amplify the response to stress and to obtain a reference model, a pure culture of Pseudomonas putida was selected instead of an activated sludge and a sole substrate (salicylic acid) was employed. The system degraded salicylic acid at 100-1100mg/L with a high level of efficiency, showed rapid acclimation without substrate or product inhibition phenomena and good stability in response to unsteady states caused by feed variations. Under starvation conditions, specific degradation rates of around 15mg/gh were achieved during the adaptation of the biomass to the new conditions and no biofilm formation was observed during the first days of experimentation using an initial substrate to microorganisms ratio lower than 0.1. When substrate was added to the reactor as pulses resulting in rapidly changing concentrations, P. putida growth was observed only for substrate to microorganism ratios higher than 0.6, with a maximum YX/S of 0.5g/g. Biofilm development under changing feeding conditions was fast, biomass detachment only being significant for biomass concentrations on the membrane surface that were higher than 16g/m(2).
authors.affiliation: Department of Chemical Engineering and Environmental Technology, University of Oviedo, E-33071 Oviedo, Spain.
authors.forename: Sergio
authors.initials: S
authors.lastname: Collado
authors.affiliation: Department of Chemical Engineering and Environmental Technology, University of Oviedo, E-33071 Oviedo, Spain.
authors.forename: Irene
authors.initials: I
authors.lastname: Rosas
authors.affiliation: Department of Chemical Engineering and Environmental Technology, University of Oviedo, E-33071 Oviedo, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: González
authors.affiliation: Department of Chemical Engineering and Environmental Technology, University of Oviedo, E-33071 Oviedo, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Gutierrez-Lavin
authors.affiliation: Department of Chemical Engineering and Environmental Technology, University of Oviedo, E-33071 Oviedo, Spain. Electronic address: mariodiaz@uniovi.es.
authors.forename: Mario
authors.initials: M
authors.lastname: Diaz
mesh_terms: D000465:Algorithms; D018441:Biofilms; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D005374:Filtration; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D016958:Pseudomonas putida / Q000187:drug effects / Q000378:metabolism*; D020156:Salicylic Acid / Q000633:toxicity*; D012722:Sewage / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D008567:Membranes, Artificial; D012722:Sewage; D014874:Water Pollutants, Chemical; D020156:Salicylic Acid
keywords: Feed induced stress; Membrane bioreactor; P. putida; Starvation; Unsteady-state