title: Whole cell biotransformation for reductive amination reactions.
abstract: Whole cell biotransformation systems with enzyme cascading increasingly find application in biocatalysis to complement or replace established chemical synthetic routes for production of, e.g., fine chemicals. Recently, we established an Escherichia coli whole cell biotransformation system for reductive amination by coupling a transaminase and an amino acid dehydrogenase with glucose catabolism for cofactor recycling. Transformation of 2-keto-3-methylvalerate to l-isoleucine by E. coli cells was improved by genetic engineering of glucose metabolism for improved cofactor regeneration. Here, we compare this system with different strategies for cofactor regeneration such as cascading with alcohol dehydrogenases, with alternative production hosts such as Pseudomonas species or Corynebacterium glutamicum, and with improving whole cell biotransformation systems by metabolic engineering of NADPH regeneration.
authors.affiliation: Chair of Genetics of Prokaryotes; Faculty of Biology & CeBiTec; Bielefeld University; Bielefeld, Germany.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Klatte
authors.affiliation: Chair of Genetics of Prokaryotes; Faculty of Biology & CeBiTec; Bielefeld University; Bielefeld, Germany.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Lorenz
authors.affiliation: Chair of Genetics of Prokaryotes; Faculty of Biology & CeBiTec; Bielefeld University; Bielefeld, Germany.
authors.forename: Volker F
authors.initials: VF
authors.lastname: Wendisch
mesh_terms: D050788:Alanine Dehydrogenase / Q000378:metabolism*; D001412:Bacillus subtilis / Q000201:enzymology*; D004926:Escherichia coli / Q000235:genetics*; D007532:Isoleucine / Q000378:metabolism*; D007651:Keto Acids / Q000378:metabolism*; D000637:Transaminases / Q000378:metabolism*
chemical_list: D007651:Keto Acids; D007532:Isoleucine; D050788:Alanine Dehydrogenase; D000637:Transaminases
keywords: Corynebacterium glutamicum; E. coli; Pseudomonas; alanine dehydrogenase; alcohol dehydrogenase; cofactor recycling; formate dehydrogenase; glucose dehydrogenase; transaminase; whole cell biotransformation