title: Structure elucidation of DNA-protein crosslinks by using reductive desulfurization and liquid chromatography-tandem mass spectrometry.
abstract: Easier with ethyl: Guengerich and co-workers have developed a powerful new approach to the structure elucidation of hydrolytically stable AGT-DNA crosslinks by reductive desulfurization of the thioether linkage between AGT and DNA to convert cysteine DPCs to the corresponding ethyl-DNA adducts, which can be readily characterized by LC-MSn.
authors.affiliation: University of Minnesota Masonic Cancer Center and the Department of Chemistry, 2231 6th Street SE, Room 2-220 CCRB, Minneapolis, MN 55455 (USA).
authors.forename: Susith
authors.initials: S
authors.lastname: Wickramaratne
authors.forename: Natalia Y
authors.initials: NY
authors.lastname: Tretyakova
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000737:chemistry; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D018736:DNA Adducts / Q000737:chemistry*; D006147:Guanine / Q000737:chemistry; D019853:O(6)-Methylguanine-DNA Methyltransferase / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction; D021241:Spectrometry, Mass, Electrospray Ionization; D013455:Sulfur / Q000737:chemistry*
chemical_list: D018736:DNA Adducts; D006147:Guanine; D013455:Sulfur; D004247:DNA; D019853:O(6)-Methylguanine-DNA Methyltransferase; D003545:Cysteine
keywords: DNA-protein crosslinks; O6-alkylguanine DNA alkyltransferase; desulfurization; labile and nonlabile DNA adducts; liquid chromatography-tandem mass spectrometry