title: [Progress of genome engineering technology via clustered regularly interspaced short palindromic repeats--a review].
abstract: In survival competition with phage, bacteria and archaea gradually evolved the acquired immune system--Clustered regularly interspaced short palindromic repeats (CRISPR), presenting the trait of transcribing the crRNA and the CRISPR-associated protein (Cas) to silence or cleaving the foreign double-stranded DNA specifically. In recent years, strong interest arises in prokaryotes primitive immune system and many in-depth researches are going on. Recently, researchers successfully repurposed CRISPR as an RNA-guided platform for sequence-specific gene expression, which provides a simple approach for selectively perturbing gene expression on a genome-wide scale. It will undoubtedly bring genome engineering into a more convenient and accurate new era.
authors.affiliation: Institute of Disease Control and Prevention of People's Liberation Army, Beijing 100071, China. lihao88663239@126.com
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.affiliation: Institute of Disease Control and Prevention of People's Liberation Army, Beijing 100071, China.
authors.forename: Shaofu
authors.initials: S
authors.lastname: Qiu
authors.affiliation: Institute of Disease Control and Prevention of People's Liberation Army, Beijing 100071, China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Song
mesh_terms: D001419:Bacteria / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D064130:CRISPR-Associated Proteins / Q000235:genetics / Q000378:metabolism; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D016680:Genome, Bacterial
chemical_list: D001426:Bacterial Proteins; D064130:CRISPR-Associated Proteins