title: Purification and viability determinations of plant protoplasts.
abstract: A method is described for purifying plant protoplasts from cellular and subcellular debris. The procedure utilizes a density buffer containing 9.6% sodium metrizoate and 5.6% Ficoll. The use of fluorescein diacetate for assessing the viability of plant protoplasts is also reported.
authors.affiliation: School of Botany, University of New South Wales, 2033, Kensington, N.S.W., Australia.
authors.forename: P J
authors.initials: PJ
authors.lastname: Larkin