title: [Study on chemical constituents of Euphorbia helioscopia and their antitumor activities].
abstract: OBJECTIVE
  To investigate the chemical constituents of Euphorbia helioscopia and their antitumor activities.
  
  
  METHODS
  Normal phase silica gel, RP-18 silica gel and Sephadex LH-20 column chromatographies combined with recrystallization were used to isolate and purify the constituents. Their structures were identifided by spectroscopic methods, including 1H-NMR, 13C-NMR, ESI-MS and EI-MS. And the antitumor activities of some of chemical constituents in vitro were detected by sulphorhodamine B protein staining.
  
  
  RESULTS
  Nine compounds were isolated and their structures were identified as euphohelioscopin A (1), euphoscopin (2), 9, 19-cyclolanost-23E-ene-3, 25-diol (3), euphoscopin C (4), euphornin A (5), euphoheliosnoid A (6), ent-kaurane-3-oxo-16beta, 17-diol (7), 9, 19-cyclolanost-25-ene-3beta, 22-diol (8) and helioscopinolide A(9) Compound 9 showed effect on inhibiting the cell proliferations of MCF-7 cell line.
  
  
  CONCLUSIONS
  Compounds 3, 7, 8 and 9 are obtained from this plant for the first time, and compound 9 shows the potential antitumor activity.
authors.affiliation: Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550022, China.
authors.forename: Shu-Zhen
authors.initials: SZ
authors.lastname: Mu
authors.affiliation: Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550022, China.
authors.forename: Shang
authors.initials: S
authors.lastname: Shang
authors.affiliation: Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550022, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Yan
authors.affiliation: Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550022, China.
authors.forename: Fu-Mei
authors.initials: FM
authors.lastname: Yang
authors.affiliation: Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550022, China.
authors.forename: Xiao-Jiang
authors.initials: XJ
authors.lastname: Hao
mesh_terms: D045784:Abietanes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology; D001943:Breast Neoplasms / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D028481:Euphorbia / Q000737:chemistry*; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry
chemical_list: D045784:Abietanes; D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; C487257:helioscopinolide A