title: Fluorescence nanoscopy. Methods and applications.
abstract: Fluorescence nanoscopy refers to the experimental techniques and analytical methods used for fluorescence imaging at a resolution higher than conventional, diffraction-limited, microscopy. This review explains the concepts behind fluorescence nanoscopy and focuses on the latest and promising developments in acquisition techniques, labelling strategies to obtain highly detailed super-resolved images and in the quantitative methods to extract meaningful information from them.
authors.affiliation: Unidad de Biofísica, CSIC-UPV/EHU, Barrio de Sarriena, s/n., 48940 Leioa, Spain.
authors.forename: Jose
authors.initials: J
authors.lastname: Requejo-Isidro
keywords: Fluorescence nanoscopy; PALM; SIM; STED; STORM; Subdiffraction imaging; Super-resolution