title: [Dynamics of ginsenoside biosynthesis in suspension culture of Panax notoginseng].
abstract: OBJECTIVE
  To study the dynamics of ginsenoside biosynthesis in suspension culture of Panax notoginseng.
  
  
  METHODS
  The calli of Panax notoginseng were suspended in liquid medium for 40 d, and the changes of ginsenosides were determined by HPLC method.
  
  
  RESULTS
  Cell growth was slow in suspension culture, but the contents of six ginsenosides varied with different culture time. The contents of four ginsenosides Rg1, Re, Rh1 and Rb1 showed the increases from about 3.63 to 9.72 mg/g, 2.34 to 7.02 mg/g, 0.42 to 2.37 mg/g and 0.24 to 1.86 mg/g, respectively during 25 and 30 days of the culture. Rh2 and F1 had no obvious change. Such results indicated that the protopanaxatriol saponions (Rg1, Re and Rh1) increased significantly during the growth phase.
  
  
  CONCLUSIONS
  When the callus is cultured for 25 days, the maximum yield of total saponins content is up to 20.58 mg/g,mainly of the protopanaxatriol saponins.
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China. wangyanwowo1986@163.com
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.
authors.forename: Yang-Yang
authors.initials: YY
authors.lastname: Wu
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Ge
authors.affiliation: Yunnan Herbal Laboratory, Institute of Herb Biotic Resources, Yunnan University, Kunming 650091, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yu
mesh_terms: D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D036145:Ginsenosides / Q000032:analysis / Q000096:biosynthesis*; D053723:Panax notoginseng / Q000254:growth & development / Q000378:metabolism*; D018517:Plant Roots / Q000166:cytology / Q000378:metabolism; D018547:Plant Stems / Q000166:cytology / Q000378:metabolism; D012639:Seeds / Q000166:cytology / Q000378:metabolism*
chemical_list: D003470:Culture Media; D036145:Ginsenosides