title: Halorientalis persicus sp. nov., an extremely halophilic archaeon isolated from a salt lake and emended description of the genus Halorientalis.
abstract: An extremely halophilic archaeon, strain D108(T), was isolated from a brine sample of Aran-Bidgol salt lake in Iran. The novel strain was cream-pigmented, motile, pleomorphic rod-shaped and required at least 2.5 M NaCl but not MgCl2 for growth. Optimal growth was achieved with 4.3 M NaCl and 0.1 M MgCl2. The optimum pH and temperature for growth were pH 7.5 and 40 °C, respectively, and the strain was able to grow over a pH range of 6.5 to 9.0, and a temperature range of 30 to 50 °C. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain D108(T) clustered with the type strain of the sole species of the genus Halorientalis, Halorientalis regularis TNN28(T), with a sequence similarity of 98.8 %. The polar lipid pattern of strain D108(T) consisted of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, one phosphoglycolipid and two glycolipids. The only quinone present was MK-8(II-H2). The DNA G+C content of strain D108(T) was 62.8 mol%. DNA-DNA hybridization studies (45 % with Halorientalis regularis IBRC-M 10760(T)), as well as biochemical and physiological characterization, allowed strain D108(T) to be differentiated from Halorientalis regularis. A novel species of the genus Halorientalis, Halorientalis persicus sp. nov., is therefore proposed to accommodate this strain. The type strain is D108(T) ( = IBRC-M 10043(T) = CECT 8375(T)). An emended description of the genus Halorientalis is also proposed.
authors.affiliation: Microorganisms Bank, Iranian Biological Resource Centre (IBRC), ACECR Tehran-Iran.|Extremophiles Laboratory, Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran.
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Amoozegar
authors.affiliation: Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Makhdoumi-Kakhki
authors.affiliation: Microorganisms Bank, Iranian Biological Resource Centre (IBRC), ACECR Tehran-Iran.|Extremophiles Laboratory, Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran.
authors.forename: Maliheh
authors.initials: M
authors.lastname: Mehrshad
authors.affiliation: Microorganisms Bank, Iranian Biological Resource Centre (IBRC), ACECR Tehran-Iran.
authors.forename: Seyed Abolhassan Shahzadeh
authors.initials: SAS
authors.lastname: Fazeli
authors.affiliation: Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
authors.forename: Cathrin
authors.initials: C
authors.lastname: Spröer
authors.affiliation: Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Ventosa
mesh_terms: D001482:Base Composition; D019641:DNA, Archaeal / Q000235:genetics; D006017:Glycolipids / Q000737:chemistry; D006216:Halobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007492:Iran; D060106:Lakes / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D014871:Water Microbiology
chemical_list: D019641:DNA, Archaeal; D006017:Glycolipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D012965:Sodium Chloride; C024223:vitamin MK 8