title: [Src homology and collagen homology (Shc) mediates autophagy induced by troglitazone in PAE cells].
abstract: OBJECTIVE
  We investigated the mechanism of Src homology and collagen homology (Shc) in autophagy caused by troglitazone (TZ).
  
  
  METHODS
  To reveal the regulatory role of p52Shc in autophagy, we used confocal microscopy and immunoblotting to examine autophagy induced by TZ. Then we used small RNA interference (siRNA) to deplete Shc and plasmids transfection to overexpress wtShc as well as 3mShc in PAE cells. Finally, we reached conclusion by detecting autophagic status following the deprivation of UNC-51-like kinase 1 (Ulk1) by siRNA.
  
  
  RESULTS
  We found that the deprivation of Shc showed to enhance autophagy, whereas p52Shc over expression suppressed TZ-depended autophagy concurring with an attenuated AMP-activated protein kinase (AMPK) and Ulk1 signaling. Besides, it demonstrated that p52Shc tyrosine sites of 239, 240 and 317 implemented a critical role in the process.
  
  
  CONCLUSIONS
  Collectively, Shc adaptor protein was involved in TZ-inducing autophagy likely via affecting AMPK and Ulk1 signaling.
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. yuandongdong000@126.com
authors.forename: Dongdong
authors.initials: D
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xiangqin
authors.initials: X
authors.lastname: Wu
authors.affiliation: China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xiuling
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001343:Autophagy / Q000187:drug effects*; D002839:Chromans / Q000494:pharmacology*; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000235:genetics / Q000378:metabolism*; D055765:Shc Signaling Adaptor Proteins / Q000235:genetics / Q000378:metabolism*; D013552:Swine; D045162:Thiazolidinediones / Q000494:pharmacology*; D000077288:Troglitazone
chemical_list: D002839:Chromans; D055765:Shc Signaling Adaptor Proteins; D045162:Thiazolidinediones; D016392:Proto-Oncogene Proteins pp60(c-src); D000077288:Troglitazone