title: An archaeal glutamate decarboxylase homolog functions as an aspartate decarboxylase and is involved in β-alanine and coenzyme A biosynthesis.
abstract: β-Alanine is a precursor for coenzyme A (CoA) biosynthesis and is a substrate for the bacterial/eukaryotic pantothenate synthetase and archaeal phosphopantothenate synthetase. β-Alanine is synthesized through various enzymes/pathways in bacteria and eukaryotes, including the direct decarboxylation of Asp by aspartate 1-decarboxylase (ADC), the degradation of pyrimidine, or the oxidation of polyamines. However, in most archaea, homologs of these enzymes are not present; thus, the mechanisms of β-alanine biosynthesis remain unclear. Here, we performed a biochemical and genetic study on a glutamate decarboxylase (GAD) homolog encoded by TK1814 from the hyperthermophilic archaeon Thermococcus kodakarensis. GADs are distributed in all three domains of life, generally catalyzing the decarboxylation of Glu to γ-aminobutyrate (GABA). The recombinant TK1814 protein displayed not only GAD activity but also ADC activity using pyridoxal 5'-phosphate as a cofactor. Kinetic studies revealed that the TK1814 protein prefers Asp as its substrate rather than Glu, with nearly a 20-fold difference in catalytic efficiency. Gene disruption of TK1814 resulted in a strain that could not grow in standard medium. Addition of β-alanine, 4'-phosphopantothenate, or CoA complemented the growth defect, whereas GABA could not. Our results provide genetic evidence that TK1814 functions as an ADC in T. kodakarensis, providing the β-alanine necessary for CoA biosynthesis. The results also suggest that the GAD activity of TK1814 is not necessary for growth, at least under the conditions applied in this study. TK1814 homologs are distributed in a wide range of archaea and may be responsible for β-alanine biosynthesis in these organisms.
authors.affiliation: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto, Japan.
authors.forename: Hiroya
authors.initials: H
authors.lastname: Tomita
authors.forename: Yuusuke
authors.initials: Y
authors.lastname: Yokooji
authors.forename: Takuya
authors.initials: T
authors.lastname: Ishibashi
authors.forename: Tadayuki
authors.initials: T
authors.lastname: Imanaka
authors.forename: Haruyuki
authors.initials: H
authors.lastname: Atomi
mesh_terms: D019843:Archaeal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001224:Aspartic Acid / Q000378:metabolism; D002262:Carboxy-Lyases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003065:Coenzyme A / Q000096:biosynthesis*; D003067:Coenzymes / Q000378:metabolism; D055786:Gene Knockout Techniques; D005968:Glutamate Decarboxylase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism; D007700:Kinetics; D011732:Pyridoxal Phosphate / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity; D019712:Thermococcus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D015091:beta-Alanine / Q000096:biosynthesis*
chemical_list: D019843:Archaeal Proteins; D003067:Coenzymes; D011994:Recombinant Proteins; D005973:Glutamine; D015091:beta-Alanine; D001224:Aspartic Acid; D011732:Pyridoxal Phosphate; D002262:Carboxy-Lyases; C019747:aspartate 4-decarboxylase; D005968:Glutamate Decarboxylase; D003065:Coenzyme A