title: The endoplasmic reticulum exerts control over organelle streaming during cell expansion.
abstract: Cytoplasmic streaming is crucial for cell homeostasis and expansion but the precise driving forces are largely unknown. In plants, partial loss of cytoplasmic streaming due to chemical and genetic ablation of myosins supports the existence of yet-unknown motors for organelle movement. Here we tested a role of the endoplasmic reticulum (ER) as propelling force for cytoplasmic streaming during cell expansion. Through quantitative live-cell analyses in wild-type Arabidopsis thaliana cells and mutants with compromised ER structure and streaming, we demonstrate that cytoplasmic streaming undergoes profound changes during cell expansion and that it depends on motor forces co-exerted by the ER and the cytoskeleton.
authors.affiliation: MSU-DOE Plant Research Lab, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Stefano
authors.forename: Luciana
authors.initials: L
authors.lastname: Renna
authors.forename: Federica
authors.initials: F
authors.lastname: Brandizzi
mesh_terms: D017360:Arabidopsis / Q000166:cytology*; D029681:Arabidopsis Proteins / Q000502:physiology; D049109:Cell Proliferation; D003595:Cytoplasmic Streaming; D004721:Endoplasmic Reticulum / Q000502:physiology*; D019204:GTP-Binding Proteins / Q000502:physiology; D007425:Intracellular Membranes / Q000378:metabolism; D008560:Membrane Fluidity
chemical_list: D029681:Arabidopsis Proteins; D019204:GTP-Binding Proteins; C464507:RHD3 protein, Arabidopsis
keywords: Arabidopsis thaliana; Cytoplasmic streaming; ER