title: Complete genome analysis identifies Tvärminne avian virus as a candidate new species within the genus Orthoreovirus.
abstract: Orthoreoviruses have been associated with a variety of diseases in domesticated poultry and wild-living birds. In 2002, a reovirus strain named Tvärminne avian virus (TVAV), was identified in Finland in a crow showing neurological disorders. The objective of this study was the molecular characterization of this novel reovirus strain. Genome sequencing was performed by combining semiconductor sequencing and traditional capillary sequencing. Sequence and phylogenetic analyses showed that TVAV shares low nucleotide sequence identity with other reoviruses (range for each gene, 31-72 %) including strains belonging to the species Avian orthoreovirus. The most closely related reovirus strain was an isolate identified in Steller sea lion. Our data indicate that TVAV is a divergent reovirus of avian origin that may be the first representative of a distinct virus species within the genus Orthoreovirus.
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Eszter
authors.initials: E
authors.lastname: Dandár
authors.affiliation: Department of Virology, Haartman Institute, Faculty of Medicine, University of Helsinki, Helsinki, Finland.
authors.forename: Eili
authors.initials: E
authors.lastname: Huhtamo
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Szilvia L
authors.initials: SL
authors.lastname: Farkas
authors.affiliation: János Szentágothai Research Center, University of Pécs, Pécs, Hungary.|Institute of Biology, Faculty of Sciences, University of Pécs, Pécs, Hungary.
authors.forename: Miklós
authors.initials: M
authors.lastname: Oldal
authors.affiliation: János Szentágothai Research Center, University of Pécs, Pécs, Hungary.|Institute of Biology, Faculty of Sciences, University of Pécs, Pécs, Hungary.
authors.forename: Ferenc
authors.initials: F
authors.lastname: Jakab
authors.affiliation: Department of Virology and Immunology, HUSLAB, Hospital District of Helsinki and Uusimaa, Helsinki, Finland.|Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland.|Department of Virology, Haartman Institute, Faculty of Medicine, University of Helsinki, Helsinki, Finland.
authors.forename: Olli
authors.initials: O
authors.lastname: Vapalahti
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Krisztián
authors.initials: K
authors.lastname: Bányai
mesh_terms: D000818:Animals; D001717:Birds; D016000:Cluster Analysis; D005387:Finland; D016679:Genome, Viral; D008969:Molecular Sequence Data; D030101:Orthoreovirus, Avian / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D012367:RNA, Viral