title: Detection and genetic characterization of human enteric viruses in oyster-associated gastroenteritis outbreaks between 2001 and 2012 in Osaka City, Japan.
abstract: Enteric viruses are an important cause of viral food-borne disease. Shellfish, especially oysters, are well recognized as a source of food-borne diseases, and oyster-associated gastroenteritis outbreaks have on occasion become international occurrences. In this study, 286 fecal specimens from 88 oyster-associated gastroenteritis outbreaks were examined for the presence of 10 human enteric viruses using antigenic or genetic detection methods in order to determine the prevalence of these infections. All virus-positive patients were over 18 years old. The most common enteric virus in outbreaks (96.6%) and fecal specimens (68.9%) was norovirus (NoV), indicating a high prevalence of NoV infection associated with the consumption of raw or under-cooked oysters. Five other enteric viruses, aichiviruses, astroviruses, sapoviruses, enteroviruses (EVs), and rotavirus A, were detected in 30.7% of outbreaks. EV strains were characterized into three rare genotypes, coxsackievirus (CV) A1, A19, and EV76. No reports of CVA19 or EV76 have been made since 1981 in the Infectious Agents Surveillance Report by the National Infectious Diseases Surveillance Center, Japan. Their detection suggested that rare types of EVs are circulating in human populations inconspicuously and one of their transmission modes could be the consumption of contaminated oysters. Rapid identification of pathogens is important for the development of means for control and prevention. The results of the present study will be useful to establish an efficient approach for the identification of viral pathogens in oyster-associated gastroenteritis in adults.
authors.affiliation: Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences, Tennoji-ku, Osaka, Japan.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Iritani
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kaida
authors.forename: Niichiro
authors.initials: N
authors.lastname: Abe
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Kubo
authors.forename: Jun-Ichiro
authors.initials: J
authors.lastname: Sekiguchi
authors.forename: Seiji P
authors.initials: SP
authors.lastname: Yamamoto
authors.forename: Kaoru
authors.initials: K
authors.lastname: Goto
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Tanaka
authors.forename: Mamoru
authors.initials: M
authors.lastname: Noda
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D004196:Disease Outbreaks; D005243:Feces / Q000821:virology; D005260:Female; D005517:Foodborne Diseases / Q000453:epidemiology* / Q000821:virology*; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology*; D006801:Humans; D007564:Japan; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010125:Ostreidae; D017422:Sequence Analysis, DNA; D014780:Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D055815:Young Adult
keywords: aichivirus; astrovirus; enterovirus; norovirus; sapovirus