title: Quantification analysis of yeast-displayed lipase.
abstract: We describe a method for quantification of displayed lipase on yeast cell surface. The strategy uses an organophosphonate ester to irreversibly inhibit the active lipase and release a detectable fluorescent group. The amount of displayed lipase can be represented as "g/g cell" or "molecules/cell". The results obtained correlated well with those obtained by existing methods. Therefore, this method is credible and will provide a powerful tool to promote research of lipase yeast surface display.
authors.affiliation: Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors.forename: Zi
authors.initials: Z
authors.lastname: Jin
authors.affiliation: Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors.forename: Sui-Ping
authors.initials: SP
authors.lastname: Zheng
authors.affiliation: Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors.forename: Shuang-Yan
authors.initials: SY
authors.lastname: Han
authors.affiliation: Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China. Electronic address: feylin@scut.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lin
mesh_terms: D061505:Cell Surface Display Techniques / Q000379:methods*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005656:Fungal Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D008049:Lipase / Q000037:antagonists & inhibitors / Q000235:genetics*; D063065:Organophosphonates / Q000494:pharmacology; D010843:Pichia / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000166:cytology*
chemical_list: D004791:Enzyme Inhibitors; D005656:Fungal Proteins; D063065:Organophosphonates; D008049:Lipase; C431349:lipase B, Candida antarctica
keywords: Active-site titration; Lipase; Quantification; Yeast surface display