title: [Optimization of succinic acid fermentation with Actinobacillus succinogenes by response surface methodology].
abstract: Succinic acid is an important C4 platform chemical in the synthesis of many commodity and special chemicals. In the present work, different compounds were evaluated for succinic acid production by Actinobacillus succinogenes GXAS 137. Important parameters were screened by the single factor experiment and Plackeet-Burman design. Subsequently, the highest production of succinic acid was approached by the path of steepest ascent. Then, the optimum values of the parameters were obtained by Box-Behnken design. The results show that the important parameters were glucose, yeast extract and MgCO3 concentrations. The optimum condition was as follows (g/L): glucose 70.00, yeast extract 9.20 and MgCO3 58.10. Succinic acid yield reached 47.64 g/L at the optimal condition. Succinic acid increased by 29.14% than that before the optimization (36.89 g/L). Response surface methodology was proven to be a powerful tool to optimize succinic acid production.
authors.affiliation: Guangxi Key Laboratory of Subtropical Bio-resource Conservation and Utilization, College of Life Science and Technology, Guangxi University, Nanning 530005, Guangxi, China.
authors.forename: Naikun
authors.initials: N
authors.lastname: Shen
authors.affiliation: National Non-grain Bio-energy Engineering Research Center, Guangxi Academy of Sciences, Nanning 530007, Guangxi, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Qin
authors.affiliation: National Non-grain Bio-energy Engineering Research Center, Guangxi Academy of Sciences, Nanning 530007, Guangxi, China.
authors.forename: Qingyan
authors.initials: Q
authors.lastname: Wang
authors.affiliation: National Non-grain Bio-energy Engineering Research Center, Guangxi Academy of Sciences, Nanning 530007, Guangxi, China.
authors.forename: Nengzhong
authors.initials: N
authors.lastname: Xie
authors.affiliation: National Non-grain Bio-energy Engineering Research Center, Guangxi Academy of Sciences, Nanning 530007, Guangxi, China.
authors.forename: Huizhi
authors.initials: H
authors.lastname: Mi
authors.affiliation: National Non-grain Bio-energy Engineering Research Center, Guangxi Academy of Sciences, Nanning 530007, Guangxi, China.
authors.forename: Qixia
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: Guangxi Key Laboratory of Subtropical Bio-resource Conservation and Utilization, College of Life Science and Technology, Guangxi University, Nanning 530005, Guangxi, China.
authors.forename: Siming
authors.initials: S
authors.lastname: Liao
authors.affiliation: Guangxi Key Laboratory of Subtropical Bio-resource Conservation and Utilization, College of Life Science and Technology, Guangxi University, Nanning 530005, Guangxi, China.
authors.forename: Ribo
authors.initials: R
authors.lastname: Huang
mesh_terms: D000188:Actinobacillus / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D019149:Bioreactors; D003470:Culture Media / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D007218:Industrial Microbiology / Q000379:methods; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: D003470:Culture Media; D019802:Succinic Acid; D005947:Glucose