title: Characterization of novel VIM carbapenemase, VIM-38, and first detection of GES-5 carbapenem-hydrolyzing β-lactamases in Pseudomonas aeruginosa in Turkey.
abstract: Pseudomonas aeruginosa isolates were collected form a Turkish hospital. Antimicrobial susceptibility was performed using the Vitek 2 Compact system, and 24 isolates were categorized as multidrug resistant (n = 18), extensively-drug resistant (n = 5), or pan-drug resistant (n = 1). PCR and DNA sequence analysis revealed that 1 strain possessed the blaGES-5 and another carried a novel blaVIM variant, named VIM-38. This new gene exhibited 1 amino acid substitution (Ala265Val) in comparison to its closest variant, VIM-5. Both VIM encoding genes were clones and demonstrated similar susceptibility profile when expressed in identical background. The presence of VIM-38 increases the diversity of carbapenemases in Turkey.
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, Bezmialem Vakıf University, Istanbul, Turkey.
authors.forename: Meryem
authors.initials: M
authors.lastname: Iraz
authors.affiliation: Department of Genetics and Bioengineering, Faculty of Engineering and Natural Sciences, Gümüşhane University, 29100 Gumushane, Turkey.
authors.forename: Azer Ozad
authors.initials: AO
authors.lastname: Duzgun
authors.affiliation: Department of Medical Microbiology, Recep Tayyip Erdogan University Faculty of Medicine, Rize, Turkey.
authors.forename: Aysegul Copur
authors.initials: AC
authors.lastname: Cicek
authors.affiliation: Service de Bactériologie-Virologie, INSERM U914, Emerging resistance to antibiotics, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine et Université Paris Sud, K-Bicêtre, France.
authors.forename: Rémy A
authors.initials: RA
authors.lastname: Bonnin
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, Bezmialem Vakıf University, Istanbul, Turkey.
authors.forename: Aysenur
authors.initials: A
authors.lastname: Ceylan
authors.affiliation: Department of Biology, Faculty of Arts & Sciences, Artvin Coruh University, 08000 Artvin, Turkey.
authors.forename: Aysegul
authors.initials: A
authors.lastname: Saral
authors.affiliation: Service de Bactériologie-Virologie, INSERM U914, Emerging resistance to antibiotics, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine et Université Paris Sud, K-Bicêtre, France.
authors.forename: Patrice
authors.initials: P
authors.lastname: Nordmann
authors.affiliation: Department of Biology, Faculty of Arts & Sciences, Recep Tayyip Erdogan University, Rize, Turkey. Electronic address: cemal.sandalli@erdogan.edu.tr.
authors.forename: Cemal
authors.initials: C
authors.lastname: Sandalli
mesh_terms: D019943:Amino Acid Substitution; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D015780:Carbapenems / Q000494:pharmacology; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000302:isolation & purification; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D014421:Turkey; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D004269:DNA, Bacterial; D001618:beta-Lactamases; C063912:carbapenemase
keywords: Carbapenemase; Class 1 integron; ESBL; Pseudomonas aeruginosa; β-lactamase