title: Therapeutic effect of Broussonetia papyrifera and Lonicera japonica in ovalbumin-induced murine asthma model.
abstract: Broussonetia papyrifera (L.) Vent. and Lonicera japonica Thunb. have been used in recent medicinal research for their antioxidative and anti-inflammatory properties. The present study investigated the therapeutic efficacy of B. papyrifera and L. japonica ethanolic extracts in a murine model of ovalbumin-induced asthma, in which intra-peritoneal (IP) injections and aerosol ovalbumin delivery were used to induce allergic asthma. Bronchioalveolar lavage fluid (BALF), serum samples, lungs and livers were collected from the experimental groups. In the groups treated with B. papyrifera and L. japonica extracts, CD3, CD4, serum IgE and IL-4 levels; activities of matrix metalloproteinase (MMP)-2 and MMP-9; and eotaxin levels in the BALF significantly decreased to near normal levels. Results of a histopathological analysis showed that the level of inflammation and mucous secretions reduced in the treated groups compared to the corresponding levels in the other groups. Moreover, results of a serum enzymatic analysis showed the non-toxic nature of the extracts in the B. papyrifera and L. japonica treated groups. Taken together, these results clearly indicate that the B. papyrifera and L. japonica extracts may be very effective against asthma and inflammation related diseases.
authors.affiliation: Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 151-921, Korea.
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Hong
authors.affiliation: Environmental Health Research Department, National Institute of Environmental Research, Incheon 404-708, Korea.
authors.forename: Jung-Taek
authors.initials: JT
authors.lastname: Kwon
authors.affiliation: Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 151-921, Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Shin
authors.affiliation: Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 151-921, Korea.
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Kim
authors.affiliation: Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 151-921, Korea.
authors.forename: Arash
authors.initials: A
authors.lastname: Minai-Tehrani
authors.affiliation: Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 151-921, Korea.
authors.forename: Kyeong-Nam
authors.initials: KN
authors.lastname: Yu
authors.affiliation: Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 151-921, Korea.
authors.forename: Somin
authors.initials: S
authors.lastname: Lee
authors.affiliation: Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 151-921, Korea.
authors.forename: Sung-Jin
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 151-921, Korea.
authors.forename: Seung-Hee
authors.initials: SH
authors.lastname: Chang
authors.affiliation: School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Hu-Lin
authors.initials: HL
authors.lastname: Jiang
authors.affiliation: Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 151-921, Korea.
authors.forename: M
authors.initials: M
authors.lastname: Vibin
authors.affiliation: Laboratory of Pathology, College of Veterinary Medicine, Seoul National University, Seoul 151-921, Korea.
authors.forename: Kiwon
authors.initials: K
authors.lastname: Han
authors.affiliation: Department of Food and Nutrition, Andong National University, Andong 760-749, Korea.
authors.forename: Kun-Ho
authors.initials: KH
authors.lastname: Son
authors.affiliation: Pharmaking, 826, Sinhung-dong, Sujeong-gu, Sungnam-city 461-160, Korea.
authors.forename: Wie-Jong
authors.initials: WJ
authors.lastname: Kwak
authors.affiliation: Laboratory of Pathology, College of Veterinary Medicine, Seoul National University, Seoul 151-921, Korea.
authors.forename: Chanhee
authors.initials: C
authors.lastname: Chae
authors.affiliation: Pharmaking, 826, Sinhung-dong, Sujeong-gu, Sungnam-city 461-160, Korea.
authors.forename: Sung-Hye
authors.initials: SH
authors.lastname: Bang
authors.affiliation: Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 151-921, Korea.
authors.forename: Myung-Haing
authors.initials: MH
authors.lastname: Cho
mesh_terms: D000818:Animals; D001249:Asthma / Q000188:drug therapy* / Q000276:immunology; D031623:Broussonetia; D004195:Disease Models, Animal; D005260:Female; D015847:Interleukin-4 / Q000032:analysis; D031239:Lonicera; D008213:Lymphocyte Activation; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use* / Q000633:toxicity
chemical_list: D010936:Plant Extracts; D015847:Interleukin-4; D010047:Ovalbumin; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9