title: Description of Pelistega indica sp. nov., isolated from human gut.
abstract: A Gram-stain-negative, motile, non-spore-forming, coccoid bacterium was isolated from a stool sample of a healthy human subject and formed cream colour colonies on tryptic soy agar. Almost full-length (1500 bp) small subunit rRNA (16S rRNA) gene sequences were generated and a similarity search was conducted by blast. The results of the similarity search indicated that the bacterium belongs to the class Betaproteobacteria, family Alcaligenaceae. It showed maximum sequence similarity (96.5 %) with Pelistega europaea CCUG 39967(T) followed by Advenella mimigardefordensis DSM 17166(T) (96.1 %) and Taylorella asinigenitalis LMG 19572(T) (95.3 %). The DNA G+C content of strain HM-7(T) was 42 mol%. Strain HM-7(T) contained C14 : 0, C16 : 0, C16 : 0 3-OH and C18 : 0 as the dominant fatty acids. Morphological, physiological and biochemical data also indicated that strain HM-7(T) represents a member of the genus Pelistega, but at the same time distinguished it from Pelistega europaea CCUG 39967(T), the only species of the genus with a validly published name. Based on polyphasic characterization we conclude that the bacterium represents a novel species of the genus Pelistega and propose the name Pelistega indica sp. nov., with strain HM-7(T) ( = MCC 2185(T) = DSM 27484(T)) as the type strain of the species.
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Pune, Maharastra 411007, India.
authors.forename: Om
authors.initials: O
authors.lastname: Prakash
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Pune, Maharastra 411007, India.
authors.forename: Hitendra
authors.initials: H
authors.lastname: Munot
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Pune, Maharastra 411007, India.
authors.forename: Yogesh
authors.initials: Y
authors.lastname: Nimonkar
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Pune, Maharastra 411007, India.
authors.forename: Madhu
authors.initials: M
authors.lastname: Sharma
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Pune, Maharastra 411007, India.
authors.forename: Shreyas
authors.initials: S
authors.lastname: Kumbhare
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Pune, Maharastra 411007, India.
authors.forename: Yogesh S
authors.initials: YS
authors.lastname: Shouche
mesh_terms: D042401:Alcaligenaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005243:Feces / Q000382:microbiology*; D041981:Gastrointestinal Tract / Q000382:microbiology; D006801:Humans; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S