title: Roseomonas rhizosphaerae sp. nov., a triazophos-degrading bacterium isolated from soil.
abstract: A novel aerobic, non-spore-forming, non-motile, catalase- and oxidase-positive, Gram-stain-negative, coccoid to short-rod-shaped bacterial strain, designated YW11(T), was isolated from soil under long-term application of triazophos. The strain was able to hydrolyse triazophos. Strain YW11(T) grew at 15-40 °C (optimum at 28 °C), at pH 5.0-8.0 (optimum at pH 7.5) and with 0-5.0 % (w/v) NaCl (optimum at 0.5 %). The major respiratory quinone was ubiquinone 10 (Q-10) and the major cellular fatty acids were C18 : 1ω7c, C16 : 0, C18 : 1 2-OH and C18 : 0. The genomic DNA G+C content of strain YW11(T) was 69.6±0.5 mol%. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, an unknown glycolipid and two unknown aminolipids. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain YW11(T) was a member of the genus Roseomonas, and showed the highest sequence similarity to Roseomonas cervicalis KACC 11686(T) (97.9 %) and Roseomonas aestuarii KACC 19645(T) (97.8 %) and then to Roseomonas ludipueritiae KACC 13843(T) (96.9 %). Strain YW11(T) showed low DNA-DNA relatedness with R. cervicalis KACC 11686(T) (32.3±2.9 %), R. aestuarii KACC 16549(T) (28.2±2.6 %) and R. ludipueritiae KACC 13843(T) (30.2±2.6 %). Based on the results of phylogenetic analysis and DNA-DNA hybridization, the whole-cell fatty acid composition as well as biochemical characteristics, strain YW11(T) was clearly distinguished from all recognized species of the genus Roseomonas and should be assigned to a novel species of the genus Roseomonas, for which the name Roseomonas rhizosphaerae sp. nov. is proposed. The type strain is YW11(T) ( = KACC 17225(T) = CCTCC AB2013041(T)).
authors.affiliation: Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Sun
authors.affiliation: Agricultural Culture Collection of China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: He
authors.affiliation: Korean Agricultural Culture Collection (KACC), National Agrobiodiversity Center, National Academy of Agricultural Science, RDA, Suwon 441-707, Republic of Korea.
authors.forename: Soon-Wo
authors.initials: SW
authors.lastname: Kwon
authors.affiliation: Jiangsu Provincial Coordinated Research Center for Organic Solid Waste Utilization, Nanjing Agricultural University, Nanjing 210095, PR China.|Key Laboratory of Plant Nutrition and Fertilization in Low-Middle Reaches of the Yangtze River, Ministry of Agriculture, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Shun-Peng
authors.initials: SP
authors.lastname: Li
authors.affiliation: Jiangsu Provincial Coordinated Research Center for Organic Solid Waste Utilization, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Jin-Gang
authors.initials: JG
authors.lastname: Gu
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D042082:Methylobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D063086:Organothiophosphates / Q000378:metabolism*; D010802:Phylogeny; D010858:Pigmentation; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism; D014230:Triazoles / Q000378:metabolism*; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D063086:Organothiophosphates; D012989:Soil Pollutants; D014230:Triazoles; D014451:Ubiquinone; C012584:triazophos; C025204:Ubiquinone Q2