title: [Effects and mechanisms of trophoblast cells autophagy in preeclampsia].
abstract: OBJECTIVE
  To investigate the occurrence law of autophagy in trophoblast cells from preeclampsia and its underlying mechanisms.
  
  
  METHODS
  Twenty cases of placenta tissues were collected from women suffered from preeclampsia and normal pregnant women respectively. Autophagosome of trophoblast cells were observed by transmission electron microscope. The expressions of LC3-II/I and Atg4B in placenta tissues were detected by western blot and real-time PCR.
  
  
  RESULTS
  Compared with the control group, typical autophagosomes of trophoblast cells were observed by transmission electron microscope. The ratio of LC3-II/I in placenta of PE patients was increased (1.43 ± 0.23) compared with control group (0.59 ± 0.12), and the expression of Atg4B was up-regulated in both mRNA [(1.73 ± 0.16) folds] and protein levels (0.71 ± 0.13) compared with control group (P < 0.05).
  
  
  CONCLUSIONS
  Autophagy was significantly up-regulated in trophoblast cells from patients suffered from preeclampsia. Thus, all the data suggest that autophagy might be involved in the generation of preeclampsia.
authors.affiliation: Department of Pharmacy, Nanjing Maternity and Child Health Hospital Affiliated to Nanjing Medical University, Nanjing 210004, China.
authors.forename: Yin-yan
authors.initials: YY
authors.lastname: Xu
authors.affiliation: Department of Pharmacy, Nanjing Maternity and Child Health Hospital Affiliated to Nanjing Medical University, Nanjing 210004, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Ding
authors.affiliation: Department of Pharmacy, Nanjing Maternity and Child Health Hospital Affiliated to Nanjing Medical University, Nanjing 210004, China.
authors.forename: Fen-fen
authors.initials: FF
authors.lastname: Zhang
authors.affiliation: Department of Pharmacy, Nanjing Maternity and Child Health Hospital Affiliated to Nanjing Medical University, Nanjing 210004, China. Email: wl_wqf@126.com.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
mesh_terms: D000328:Adult; D001343:Autophagy; D000071183:Autophagy-Related Proteins; D016022:Case-Control Studies; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D011225:Pre-Eclampsia / Q000378:metabolism* / Q000473:pathology; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D014327:Trophoblasts / Q000166:cytology* / Q000473:pathology / Q000648:ultrastructure; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D000071183:Autophagy-Related Proteins; C477012:MAP1LC3A protein, human; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; C507524:ATG4B protein, human; D003546:Cysteine Endopeptidases