title: Efficient programming of human eye conjunctiva-derived induced pluripotent stem (ECiPS) cells into definitive endoderm-like cells.
abstract: Due to pluripotency of induced pluripotent stem (iPS) cells, and the lack of immunological incompatibility and ethical issues, iPS cells have been considered as an invaluable cell source for future cell replacement therapy. This study was aimed first at establishment of novel iPS cells, ECiPS, which directly reprogrammed from human Eye Conjunctiva-derived Mesenchymal Stem Cells (EC-MSCs); second, comparing the inductive effects of Wnt3a/Activin A biomolecules to IDE1 small molecule in derivation of definitive endoderm (DE) from the ECiPS cells. To that end, first, the EC-MSCs were transduced by SOKM-expressing lentiviruses and characterized for endogenous expression of embryonic markers Then the established ECiPS cells were induced to DE formation by Wnt3a/Activin A or IDE1. Quantification of GSC, Sox17 and Foxa2 expression, as DE-specific markers, in both mRNA and protein levels revealed that induction of ECiPS cells by either Wnt3a/Activin A or IDE1 could enhance the expression level of the genes; however the levels of increase were higher in Wnt3a/Activin A induced ECiPS-EBs than IDE1 induced cells. Furthermore, the flow cytometry analyses showed no synergistic effect between Activin A and Wnt3a to derive DE-like cells from ECiPS cells. The comparative findings suggest that although both Wnt3a/Activin A signaling and IDE1 molecule could be used for differentiation of iPS into DE cells, the DE-inducing effect of Wnt3a/Activin A was statistically higher than IDE1.
authors.affiliation: Induced Pluripotent Stem Cell Biotechnology Team, Stem Cells Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran; Stem Cells Biology Department, Stem Cell Technology Research Center, Tehran, Iran; Department of Physiology, University of Toronto, Toronto, Ontario, Canada. Electronic address: massumi.mohammad@gmail.com.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Massumi
authors.affiliation: Department of Biology, Faculty of Sciences, Kharazmi University (Tarbiat Moallem), Tehran, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Hoveizi
authors.affiliation: Induced Pluripotent Stem Cell Biotechnology Team, Stem Cells Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
authors.forename: Parvaneh
authors.initials: P
authors.lastname: Baktash
authors.affiliation: Induced Pluripotent Stem Cell Biotechnology Team, Stem Cells Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
authors.forename: Abdollah
authors.initials: A
authors.lastname: Hooti
authors.affiliation: Induced Pluripotent Stem Cell Biotechnology Team, Stem Cells Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
authors.forename: Leili
authors.initials: L
authors.lastname: Ghazizadeh
authors.affiliation: Stem Cells Biology Department, Stem Cell Technology Research Center, Tehran, Iran.
authors.forename: Samad
authors.initials: S
authors.lastname: Nadri
authors.affiliation: Department of Biotechnology, Razi Vaccine and Serum Research Center, Karaj, Iran; Molecular Biology and Genetic Engineering Department, Stem Cell Technology Research Center, Tehran, Iran.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Pourasgari
authors.affiliation: Molecular Biology and Genetic Engineering Department, Stem Cell Technology Research Center, Tehran, Iran.
authors.forename: Athena
authors.initials: A
authors.lastname: Hajarizadeh
authors.affiliation: Department of Hematology, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
authors.affiliation: Department of Biology, Faculty of Sciences, Kharazmi University (Tarbiat Moallem), Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Nabiuni
authors.affiliation: Department of Medical Biotechnology, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: khoramza@sina.tums.ac.ir.
authors.forename: Mohammad R
authors.initials: MR
authors.lastname: Khorramizadeh
mesh_terms: D028341:Activins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D054338:Cell Transdifferentiation / Q000502:physiology*; D002478:Cells, Cultured; D065150:Cellular Reprogramming / Q000502:physiology; D003228:Conjunctiva / Q000166:cytology* / Q000378:metabolism; D004707:Endoderm / Q000166:cytology / Q000502:physiology*; D057809:HEK293 Cells; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000502:physiology*; D051379:Mice; D060509:Wnt3A Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D015415:Biomarkers; D060509:Wnt3A Protein; C422405:activin A; D028341:Activins
keywords: Activin A; Definitive endoderm; IDE1; Induced pluripotent stem cells; Wnt3a