title: Characterization of structural and functional properties of fish protein hydrolysates from surimi processing by-products.
abstract: Structural and functional properties of fish protein hydrolysates with different degrees of hydrolysis (DH) from surimi processing by-products, prepared by Protamex and Alcalase, were evaluated. As the DH increased, the zeta potentials of the hydrolysates increased (p>0.05). The surface hydrophobicity of the hydrolysates was significantly affected by DH (p<0.05). A wide variety of peptides were obtained after hydrolysis by Protamex and Alcalase. The hydrolysate with DH 10%, prepared by Protamex, contained more large protein molecules than did the others. Hydrolysis by both enzymes increased solubility to more than 65% over a wide pH range (pH 2-10). The interfacial activities of hydrolysates decreased with increasing DH (p<0.05). The hydrolysate with DH 10%, prepared by Protamex, exhibited the best interfacial properties among all of the samples. Thermal properties were also affected by the hydrolysis. The results reveal that structures and functionalities of the hydrolysates were determined both by DH and enzyme type employed.
authors.affiliation: School of Chemical and Biological Engineering, Changsha University of Science & Technology, 960 South Wanjiali Road, 2nd Section, Changsha 410004, Hunan Province, PR China.
authors.forename: Yongle
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Chemical and Biological Engineering, Changsha University of Science & Technology, 960 South Wanjiali Road, 2nd Section, Changsha 410004, Hunan Province, PR China. Electronic address: Xianghongl@163.com.
authors.forename: Xianghong
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Chemical and Biological Engineering, Changsha University of Science & Technology, 960 South Wanjiali Road, 2nd Section, Changsha 410004, Hunan Province, PR China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Chen
authors.affiliation: School of Chemical and Biological Engineering, Changsha University of Science & Technology, 960 South Wanjiali Road, 2nd Section, Changsha 410004, Hunan Province, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yu
authors.affiliation: School of Chemical and Biological Engineering, Changsha University of Science & Technology, 960 South Wanjiali Road, 2nd Section, Changsha 410004, Hunan Province, PR China.
authors.forename: Faxiang
authors.initials: F
authors.lastname: Wang
authors.affiliation: School of Chemical and Biological Engineering, Changsha University of Science & Technology, 960 South Wanjiali Road, 2nd Section, Changsha 410004, Hunan Province, PR China.
authors.forename: Jianhui
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D005399:Fishes; D006868:Hydrolysis; D011492:Protein Hydrolysates / Q000737:chemistry*
chemical_list: D011492:Protein Hydrolysates
keywords: Degrees of hydrolysis; Fish protein hydrolysate; Functional property; Structure