title: Sulcus fluid bone marker levels and the outcome of surgical treatment of peri-implantitis.
abstract: OBJECTIVE
  To analyse change in selected bone markers in peri-implant sulcus fluid (PISF) sampled before treatment and after 12 months and test correlation with change in disease progression.
  
  
  METHODS
  Peri-implant sulcus fluid was sampled from 32 patients in a randomized, clinical study comparing peri-implant defect re-construction with or without porous titanium granules. Matrix metalloproteinase 8 levels were measured using the Quantikine Human Total MMP-8 (DMP800) ELISA. Multianalyte profiling of the level of bone markers [interleukin-6, osteprotegerin (OPG), osteocalcin, leptin, osteopontin, parathyroid hormone, tumour necrosis factor-α, adiponectin and insulin] was performed by Luminex using Human Bone Panel IB. Changes in bone marker levels were compared and correlation with clinical findings was tested.
  
  
  RESULTS
  No differences in clinical parameter or bone marker levels between test and control group were found. When comparing bone marker levels irrespective of treatment allocation between baseline and 12 months, a significant reduction in total protein, matrix metalloproteinase -8, interleukin-6, OPG, leptin and adiponectin were demonstrated. Positive correlations were found between the reduction in interleukin-6 (r = 0.43), insulin (r = 0.38) and matrix metalloproteinase-8 (r = 0.47) concentration, and probing pocket depth reduction.
  
  
  CONCLUSIONS
  Peri-implantitis surgical treatment induced some reduction of the studied bone markers. Conclusive evidence for correlation between change in bone marker concentrations with disease resolution was not found.
authors.affiliation: Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, Oslo, Norway; Department of Periodontology, Institute of Clinical Dentistry, University of Oslo, Oslo, Norway.
authors.forename: Johan C
authors.initials: JC
authors.lastname: Wohlfahrt
authors.forename: Anne M
authors.initials: AM
authors.lastname: Aass
authors.forename: Finn
authors.initials: F
authors.lastname: Granfeldt
authors.forename: Ståle P
authors.initials: SP
authors.lastname: Lyngstadaas
authors.forename: Janne E
authors.initials: JE
authors.lastname: Reseland
mesh_terms: D052242:Adiponectin / Q000032:analysis; D001672:Biocompatible Materials / Q000737:chemistry; D015415:Biomarkers / Q000032:analysis; D003646:Debridement / Q000379:methods; D015921:Dental Implants; D018450:Disease Progression; D005500:Follow-Up Studies; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D007328:Insulin / Q000032:analysis; D015850:Interleukin-6 / Q000032:analysis; D020738:Leptin / Q000032:analysis; D020784:Matrix Metalloproteinase 8 / Q000032:analysis; D015675:Osteocalcin / Q000032:analysis; D053495:Osteopontin / Q000032:analysis; D053244:Osteoprotegerin / Q000032:analysis; D010281:Parathyroid Hormone / Q000032:analysis; D057873:Peri-Implantitis / Q000601:surgery*; D010514:Periodontal Pocket / Q000601:surgery; D011446:Prospective Studies; D019651:Plastic Surgery Procedures / Q000379:methods; D013524:Surgical Flaps / Q000601:surgery; D014025:Titanium / Q000737:chemistry; D016896:Treatment Outcome; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: C499918:ADIPOQ protein, human; D052242:Adiponectin; D001672:Biocompatible Materials; D015415:Biomarkers; D015921:Dental Implants; C508600:IL6 protein, human; D007328:Insulin; D015850:Interleukin-6; D020738:Leptin; D053244:Osteoprotegerin; C470125:PTH protein, human; D010281:Parathyroid Hormone; C506030:TNFRSF11B protein, human; D014409:Tumor Necrosis Factor-alpha; D015675:Osteocalcin; D053495:Osteopontin; D014025:Titanium; C488762:MMP8 protein, human; D020784:Matrix Metalloproteinase 8
keywords: bone markers; dental implants; peri-implantitis; surgical treatment