title: Expression of SNMP1 and SNMP2 genes in antennal sensilla of Spodoptera exigua (Hübner).
abstract: Sensory neuron membrane proteins (SNMPs) are olfactory-specific, two-transmembrane proteins. Previous publications reported that SNMP1 is expressed on the dendrite membrane of pheromone-sensitive neurons in Heliothis virescens and is an essential cofactor for pheromone detection in Drosophila. In this study, we cloned two SNMP genes (GenBank accession nos. JX469106 and JX469107) from the antenna of the beet armyworm moth Spodoptera exigua (Lepidoptera: Hübner). These SNMP genes are classified into two highly conserved subclades, indicating their importance in physiological activity of lepidopteran insects. SexiSNMP1 is antenna-specific in male and female adults, while SexiSNMP2 is antenna-abundant but also expressed in other chemosensory tissues, particularly proboscises and maxillary palps of adults both sexes. In situ hybridization revealed that both SNMPs are broadly expressed in long and short trichoid and basiconic sensilla. We infer that SNMP1 and SNMP2 act in the detection of the sex pheromone and general odorants.
authors.affiliation: Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing, China; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Liu
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Guirong
authors.initials: G
authors.lastname: Wang
authors.forename: Shuanglin
authors.initials: S
authors.lastname: Dong
mesh_terms: D000818:Animals; D057506:Arthropod Antennae / Q000378:metabolism*; D001483:Base Sequence; D017931:DNA Primers; D017344:Genes, Insect; D017403:In Situ Hybridization; D019476:Insect Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D018411:Spodoptera / Q000235:genetics*
chemical_list: D017931:DNA Primers; D019476:Insect Proteins
keywords: Spodoptera exigua; in situ hybridization; olfaction; sensillum; sensory neuron membrane protein