title: A model system for targeted drug release triggered by biomolecular signals logically processed through enzyme logic networks.
abstract: A new Sense-and-Act system was realized by the integration of a biocomputing system, performing analytical processes, with a signal-responsive electrode. A drug-mimicking release process was triggered by biomolecular signals processed by different logic networks, including three concatenated AND logic gates or a 3-input OR logic gate. Biocatalytically produced NADH, controlled by various combinations of input signals, was used to activate the electrochemical system. A biocatalytic electrode associated with signal-processing "biocomputing" systems was electrically connected to another electrode coated with a polymer film, which was dissolved upon the formation of negative potential releasing entrapped drug-mimicking species, an enzyme-antibody conjugate, operating as a model for targeted immune-delivery and consequent "prodrug" activation. The system offers great versatility for future applications in controlled drug release and personalized medicine.
authors.affiliation: Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY 13676, USA. ekatz@clarkson.edu.
authors.forename: Shay
authors.initials: S
authors.lastname: Mailloux
authors.forename: Jan
authors.initials: J
authors.lastname: Halámek
authors.forename: Evgeny
authors.initials: E
authors.lastname: Katz
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D016503:Drug Delivery Systems / Q000379:methods*; D008128:Logic; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D060066:Protein Interaction Maps / Q000502:physiology; D017433:Protein Structure, Secondary