title: Rapid identification of bacteria and Candida pathogens in peritoneal dialysis effluent from patients with peritoneal dialysis-related peritonitis by use of multilocus PCR coupled with electrospray ionization mass spectrometry.
abstract: PCR coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was compared with culture for pathogen detection in peritoneal dialysis (PD)-related peritonitis. Of 21 samples of PD effluent, PCR/ESI-MS identified microorganisms in 18 (86%) samples, including Mycobacterium tuberculosis in 1 culture-negative sample. Of 15 double-positive samples, PCR/ESI-MS and culture reached levels of agreement of 100% (15/15) and 87.5% (7/8) at the genus and species levels, respectively. PCR/ESI-MS can be used for rapid pathogen detection in PD-related peritonitis.
authors.affiliation: Department of Internal Medicine, National Cheng Kung University Hospital and College of Medicine, Tainan, Taiwan.
authors.forename: Yu-Tzu
authors.initials: YT
authors.lastname: Chang
authors.forename: Hsuan-Chen
authors.initials: HC
authors.lastname: Wang
authors.forename: Ming-Cheng
authors.initials: MC
authors.lastname: Wang
authors.forename: An-Bang
authors.initials: AB
authors.lastname: Wu
authors.forename: Junne-Ming
authors.initials: JM
authors.lastname: Sung
authors.forename: H Sunny
authors.initials: HS
authors.lastname: Sun
authors.forename: Ih-Jen
authors.initials: IJ
authors.lastname: Su
authors.forename: Wei-Chih
authors.initials: WC
authors.lastname: Kan
authors.forename: Chih-Chiang
authors.initials: CC
authors.lastname: Chien
authors.forename: Jyh-Chang
authors.initials: JC
authors.lastname: Hwang
authors.forename: Hsien-Yi
authors.initials: HY
authors.lastname: Wang
authors.forename: Chin-Chung
authors.initials: CC
authors.lastname: Tseng
authors.forename: Chi-Jung
authors.initials: CJ
authors.lastname: Wu
mesh_terms: D000328:Adult; D000368:Aged; D001419:Bacteria / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D002175:Candida / Q000302:isolation & purification*; D002177:Candidiasis / Q000175:diagnosis* / Q000382:microbiology; D002648:Child; D015314:Dialysis Solutions; D005260:Female; D006801:Humans; D008297:Male; D008828:Microbiological Techniques / Q000379:methods*; D008875:Middle Aged; D010530:Peritoneal Dialysis / Q000009:adverse effects; D010538:Peritonitis / Q000175:diagnosis* / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D055815:Young Adult
chemical_list: D015314:Dialysis Solutions