title: Sequence related amplified polymorphism (SRAP) analysis for genetic diversity and micronutrient content among gene pools in mungbean [Vigna radiata (L.) Wilczek].
abstract: Vigna radiata (L.) Wilczek, commonly called mungbean is an important pulse crop. Commercial cultivars contain low levels of iron and zinc and it is important to assess genetic variability in the available germplasm for improving micronutrient content in commercial cultivars. The present study was undertaken to study molecular diversity using Sequence-related amplified polymorphism (SRAP) among 21 Vigna radiata genotypes. Twenty nine SRAP primer combinations produced a total of 121 amplified bands which were polymorphic with an average of 4.65 bands per primer. The size of amplified bands ranged from 70 bp to 3,000 bp and 6 out of 29 SRAP primers were most useful in fingerprinting Vigna radiata genotypes under study. The similarity coefficients between different genotypes ranged from 0.45 to 0.96 with an average similarity value of 0.71. At an arbitrary cut-off at 60 % similarity level on a dendrogram, the Vigna radiata accessions were categorized into two major clusters. ML1108 and 2KM115 were found to be genetically similar. SMH99-1A and ML776 showed high iron and zinc content while Satya was poor in iron as well as zinc content. Mapping population involving ML776 and Satya could be used for tagging gene(s) for micronutrient content. The results indicated that SRAP markers were efficient for identification of Vigna radiata genotypes and assessment of the genetic relationships among them.
authors.affiliation: Department of Molecular Biology and Biotechnology, Chaudhary Charan Singh Haryana Agricultural University, Hisar, 125004 India.
authors.forename: Bharti
authors.initials: B
authors.lastname: Aneja
authors.affiliation: Department of Molecular Biology and Biotechnology, Chaudhary Charan Singh Haryana Agricultural University, Hisar, 125004 India.
authors.forename: Neelam R
authors.initials: NR
authors.lastname: Yadav
authors.affiliation: Department of Molecular Biology and Biotechnology, Chaudhary Charan Singh Haryana Agricultural University, Hisar, 125004 India.
authors.forename: Ram C
authors.initials: RC
authors.lastname: Yadav
authors.affiliation: Department of Genetics and Plant Breeding, Chaudhary Charan Singh Haryana Agricultural University, Hisar, 125004 India.
authors.forename: Ram
authors.initials: R
authors.lastname: Kumar
keywords: Genetic variability; Iron and zinc content; Mungbean; SRAP