title: [Effect of curcumin on expression of AKT and p-AKT in hippocampus CA1 area of App/PS1 double transgenic mice].
abstract: OBJECTIVE
  To observe the effect of curcumin on the expressions of AKT (serine-threonine kinase, AKT, also known as PKB) and p-AKT (phosphated serine-threonine kinase, p-AKT) in APP/PS1 double transgenic mice of the AD model.
  
  
  METHODS
  Three-month-old APP/PS1 double transgenic mice were randomly divided into the model group, the rosiglitazone (10 mg kg-1 . d-1) group, and high (400 mg . kg-1 d-1), medium (200 mg . kg-1 d-1) and low (100 mg kg-1 d-1) dosecurcumin groups. Non-transgenic mice of the same age and background were selected as the control group ( n = 12). After all of the six groups were intragastrically administered for consecutively three months, the protein expressions of AKT and p-AKT in hippocampus CA1 area were detected by immunohistochemistry and Western blot.
  
  
  RESULTS
  The results of immunohistochemistry showed that the expression of AKT and p-AKT positive cells in hippocampus CA1 area significantly decreased in the model group (P <0. 05 and P < 0. 01). Compared with the model group, AKT and p-AKT positive cells of hippocampus CA1 area increased obviously in the rosiglitazone group and high and medium dose curcumin group (P <0.05 or P <0.01) ,especially the medium dose group (P <0.01). The results of Western blot were consistent with that of immunohistochemistry.
  
  
  CONCLUSIONS
  Curcumin can recover the decreased AKT and p-AKT cells in hippocampus CAl area of APP/PS1 double transgenic mice of the AD model, suggesting that curcumin may regulate AKT and its phosphorylation process, as well as PI3K/AKT insulin signal transduction pathway, and show the anti-AD effect.
authors.affiliation: Laboratory of Pharmacology of Dongzhimen Hospital, Beijing University of Chinese Medicine, Tertiary Laboratory of State Administration of Traditional Chinese Medicine, Beijing 100700, China. danghz@163.com
authors.forename: Hui-Zi
authors.initials: HZ
authors.lastname: Dang
authors.affiliation: The Second Hospital Affiliated to Xinjiang Medical University, Urumqi 830028, China.
authors.forename: Rui-Sheng
authors.initials: RS
authors.lastname: Li
authors.affiliation: Amcare Women and Children's Hospital, Beijing 100016, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Laboratory of Pharmacology of Dongzhimen Hospital, Beijing University of Chinese Medicine, Tertiary Laboratory of State Administration of Traditional Chinese Medicine, Beijing 100700, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ren
authors.affiliation: Laboratory of Pharmacology of Dongzhimen Hospital, Beijing University of Chinese Medicine, Tertiary Laboratory of State Administration of Traditional Chinese Medicine, Beijing 100700, China.
authors.forename: Hai-Yun
authors.initials: HY
authors.lastname: Sun
authors.affiliation: Laboratory of Pharmacology of Dongzhimen Hospital, Beijing University of Chinese Medicine, Tertiary Laboratory of State Administration of Traditional Chinese Medicine, Beijing 100700, China.
authors.forename: Jin-Duo
authors.initials: JD
authors.lastname: Yang
authors.affiliation: Laboratory of Pharmacology of Dongzhimen Hospital, Beijing University of Chinese Medicine, Tertiary Laboratory of State Administration of Traditional Chinese Medicine, Beijing 100700, China.
authors.forename: Peng-Wen
authors.initials: PW
authors.lastname: Wang
mesh_terms: D016564:Amyloid beta-Protein Precursor / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D056547:CA1 Region, Hippocampal / Q000187:drug effects* / Q000201:enzymology*; D003474:Curcumin / Q000494:pharmacology*; D007150:Immunohistochemistry; D051379:Mice; D008822:Mice, Transgenic; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics
chemical_list: D016564:Amyloid beta-Protein Precursor; D017346:Protein Serine-Threonine Kinases; D003474:Curcumin