title: A method for molecular analysis of catalase gene diversity in seawater.
abstract: Catalase plays an important role in the metabolism of marine bacteria and has potential impact on the marine environment. Four PCR primers were designed to amplify the catalase gene fragments in marine bacteria by applying metagenomic DNA from Yellow Sea surface water as the template. Of the four reproducible target PCR products, the longest one with 900 bp were chosen for catalase gene library construction by the T-vector and the white Escherichia coli colonies in the library was screened through restriction-digesting the reamplified insert fragments by the selected restriction endonuclease MboI, and then the bands of the resulting products were displayed in the agarose gel by electrophoresis. The unique restriction fragment length polymorphism (RFLP) pattern was selected and the corresponding catalase gene fragments were sequenced, which verified that every unique RFLP pattern represented one type of catalase. This PCR-RFLP method above was established to investigate the bacterial catalase diversity in seawater.
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao, 266071 China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao, 266071 China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Ji
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao, 266071 China.
authors.forename: Cui
authors.initials: C
authors.lastname: Yuan
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao, 266071 China.
authors.forename: Fangqun
authors.initials: F
authors.lastname: Dai
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao, 266071 China.
authors.forename: Jiancheng
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao, 266071 China ; Marine Products Resource and Enzyme Engineering Laboratory, Yellow Sea Fisheries Research Institute, 106 Nanjing Road, Qingdao, 266071 Shandong China.
authors.forename: Mi
authors.initials: M
authors.lastname: Sun
keywords: Hydrogen peroxidase; Marine bacteria; PCR–RFLP method; Surface seawater