title: Effect of various growth hormone concentration and combination on callus induction, nature of callus and callogenic response of Nerium odorum.
abstract: Nerium odorum, Linn. (Apocynaceae) is an important evergreen shrub. It is heat, salinity and drought tolerant. Plants with milky sap have medicinal value, mainly cardenolides, flavonoids and terpenes. It is used for wastewater purification and for restoration of riparian woodlands. In view of these facts, the study was conducted for micropropagation of N. odorum. Murashige and Skoog (MS) media supplemented with different concentrations (0.5-10.0 mg/l) of 1-naphthaleneacetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BAP) and kinetin (Kin) were used singly and in combinations. Among all the growth hormones, 2,4-D was the best for callus induction (75% in stem and 79% in leaf) and in combination 2,4-D and BAP (78% in stem and 81% in leaf). The day of callus induction started from the 19th to the 37th day. This variation is due to the differences in culture conditions and the age of explants. The fresh and dry weight and moisture content showed good growth of callus, which is used in further studies of alkaloid production. Micropropagation of this plant allows the production of clones at a fast rate and in continuous manner. This work can lead to the development of an efficient protocol for callus induction and other issues.
authors.affiliation: Department of Botany, Faculty of Science, Patna Science College, Patna University, Patna, Bihar, 800005, India, runa@zhi.org.in.
authors.forename: Runa
authors.initials: R
authors.lastname: Rashmi
authors.forename: Maheshwar Prasad
authors.initials: MP
authors.lastname: Trivedi
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D000470:Alkaloids / Q000096:biosynthesis*; D001593:Benzyl Compounds; D003470:Culture Media; D004338:Drug Combinations; D007701:Kinetin / Q000494:pharmacology; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D030007:Nerium / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D058439:Plant Somatic Embryogenesis Techniques; D018547:Plant Stems / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D011687:Purines
chemical_list: D000470:Alkaloids; D001593:Benzyl Compounds; D003470:Culture Media; D004338:Drug Combinations; D009280:Naphthaleneacetic Acids; D010937:Plant Growth Regulators; D011687:Purines; D015084:2,4-Dichlorophenoxyacetic Acid; C034182:1-naphthaleneacetic acid; C480551:benzylaminopurine; D007701:Kinetin