title: Interrelationship between NO and androgenic activity in mice, Mus musculus, following temporal phase relation of serotonergic and dopaminergic neural oscillations.
abstract: The present study was designed to monitor the correlation of gonadal steroidogenic activity and nitric oxide (NO) of plasma and testis of male laboratory mouse, Mus musculus, under (1) control/basal and (2) experimental conditions. In the first study, male mice (n = 6) of three age groups (3-week-old sexually immature, 9-week-old sexually mature and 1.5-year-old) were assayed for plasma testosterone and level of NO in the plasma and testis. The immunoreactivity of 3-β-hydroxysteroid dehydrogenase (3-β-HSD) and androgen receptors (ARs) was also detected in testis and epididymis, respectively. In the second study, the reproductive state of mice was altered by injecting precursors of serotonin 5-hydroxytryptophan (5-HTP) and dopamine L-dihydroxyphenylalanine (L-DOPA) daily for 13 days. In one subgroup, mice received their daily injections 8 h apart and in another subgroup 12 h apart. A third subgroup of control mice received two daily injections of normal saline. After the completion of the experiment, the mentioned parameters were measured. The results showed a significant increase in levels of NO and a lowering of the immune reactivity of 3-β-HSD and AR, in both leydig cells and the epididymis in the sexually immature, old-age mice as well as in the mice that received 5-HTP and L-DOPA in the 8-h temporal relation, whereas opposite effects were observed in the sexually mature mice as well as in the mice that received 5-HTP and L-DOPA in the 12-h temporal relation. These findings led us to conclude that an inverse correlation exists between testicular steroidogenic activity and NO activity of laboratory mice under control and experimentally modulated gonadal conditions.
authors.affiliation: Department of Zoology, Banaras Hindu University, Varanasi, 221005, UP, India.
authors.forename: Vineet Prakash
authors.initials: VP
authors.lastname: Singh
authors.forename: Chandra Mohini
authors.initials: CM
authors.lastname: Chaturvedi
mesh_terms: D006916:5-Hydroxytryptophan / Q000494:pharmacology; D000367:Age Factors; D000818:Animals; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D004298:Dopamine / Q000378:metabolism*; D007980:Levodopa / Q000494:pharmacology; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000097:blood / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism*; D013737:Testis / Q000187:drug effects / Q000378:metabolism*; D013739:Testosterone / Q000097:blood / Q000378:metabolism*
chemical_list: D009569:Nitric Oxide; D012701:Serotonin; D013739:Testosterone; D007980:Levodopa; D006916:5-Hydroxytryptophan; D004298:Dopamine