title: Fabricating antigen recognition and anti-bioadhesion polymeric surface via a photografting polymerization strategy.
abstract: A polymeric platform for immunodiagnostic bioassay was constructed based on biostable polymeric support and two functional monomers, low-fouling methacryloyloxyethyl phosphorylcholine (MPC) and acrylic acid (AAc), by a photografting polymerization approach. Covalent binding of antibody to pAAc chains was achieved by activating carboxyl with NHS/EDC partner. The resultant surface showed obvious suppression of nonspecific protein adsorption and platelet adhesion relative to the control sample, exhibiting good anti-bioadhesion performances. Based on the polymer-supported matrix, a highly sensitive antibody-antigen specific recognition was confirmed in both native plasma and diluted human plasma due to the enhanced antibody loading capacity and lowered bioadhesion as compared to the reference.
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Lingjie
authors.initials: L
authors.lastname: Song
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
authors.forename: Jingchuan
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China. Electronic address: sfluan@ciac.ac.cn.
authors.forename: Shifang
authors.initials: S
authors.lastname: Luan
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China. Electronic address: yinjh@ciac.ac.cn.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yin
mesh_terms: D000179:Acrylates / Q000737:chemistry; D000268:Adhesiveness; D000327:Adsorption; D000906:Antibodies / Q000378:metabolism; D000941:Antigens / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005340:Fibrinogen / Q000378:metabolism; D006801:Humans; D055767:Immobilized Proteins / Q000378:metabolism; D008027:Light; D009113:Muramidase / Q000378:metabolism; D010767:Phosphorylcholine / Q000031:analogs & derivatives / Q000737:chemistry; D056951:Photoelectron Spectroscopy; D010973:Platelet Adhesiveness; D058105:Polymerization / Q000528:radiation effects*; D011108:Polymers / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000179:Acrylates; D000906:Antibodies; D000941:Antigens; D055767:Immobilized Proteins; D011108:Polymers; D011109:Polymethacrylic Acids; C115766:poly(2-methacryloyloxyethyl-phosphorylcholine); D010767:Phosphorylcholine; D012710:Serum Albumin, Bovine; D005340:Fibrinogen; D009113:Muramidase; C036658:acrylic acid
keywords: Anti-bioadhesion; Antibody immobilization; Photografting polymerization