title: Partial purification and properties of pectin lyase from Penicillium expansum.
abstract: A pectin lyase, poly(methoxygalacturonide) lyase, EC 4.2.2.10, from a culture filtrate of Penicillium expansum was partially purified 33-fold with 7.3% yield. The enzyme was monomeric with a molecular mass of 36.5 kDa. The enzyme did not contain pectate lyase activity and degraded citrus and apple pectin best at pH 7.0 and 40 to 45°C. The K m for citrus pectin was 9 mg ml(-1).
authors.affiliation: Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, P.O. Box 594, S10 2UH, Sheffield, UK.
authors.forename: D O
authors.initials: DO
authors.lastname: Silva
authors.forename: M M
authors.initials: MM
authors.lastname: Attwood
authors.forename: D W
authors.initials: DW
authors.lastname: Tempest