title: Effects of different scaffolds on rat adipose tissue derived stroma cells.
abstract: BACKGROUND
  Adipose tissue derived stroma cells (ASC's) offer for many advantages for tissue engineering strategies over mesenchymal stroma cells from other sources and ideal carrier materials have to be identified for them. The aim of this study was to demonstrate and to compare the effects of three clinically established biomaterials on proliferation and metabolic activity of rat ASC's in vitro.
  
  
  METHODS
  Rat adipose tissue derived stroma cells (ASC's) were isolated and differentiated into distinct lineages proved by lineage specific staining and gene expression analysis (RT-PCR). The biomaterials Bio-Gide(®), Tutodent(®) Membrane and Belotero(®) Soft were tested with rat ASC's for their biocompatibility using scanning electron microscopy (SEM), cell vitality staining, cytotoxicity and proliferation tests (LDH, MTT, BrdU, WST-1).
  
  
  RESULTS
  The collagen membrane Bio-Gide(®) resulted in a significantly higher viability and proliferation (WST-1, BrdU) compared to Tutodent(®) Membrane. No significant difference was determined in the LDH and MTT test. The hyaluronic acid gel Belotero(®) Soft showed no cytotoxicity (LDH, FDA/PI) and had no negative effects on metabolic activity (WST-1, MTT) or cell proliferation (BrdU) of ASC's.
  
  
  CONCLUSIONS
  Our results indicate Bio-Gide(®) and Belotero(®) Soft as preferable carrier materials for ASC's. For the further establishment of ASC's-based treatment strategies, in vivo investigations on the tissue regeneration potential of these cell-biomaterial scaffolds should follow.
authors.affiliation: Department of Oral & Maxillofacial Surgery, Christian-Albrechts-University, Arnold-Heller-Str. 16, 24105 Kiel, Germany.
authors.forename: Yahya
authors.initials: Y
authors.lastname: Açil
authors.affiliation: Department of Oral & Maxillofacial Surgery, Christian-Albrechts-University, Arnold-Heller-Str. 16, 24105 Kiel, Germany.
authors.forename: Xiacong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Oral & Maxillofacial Surgery, Christian-Albrechts-University, Arnold-Heller-Str. 16, 24105 Kiel, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Nitsche
authors.affiliation: Department of Oral & Maxillofacial Surgery, Christian-Albrechts-University, Arnold-Heller-Str. 16, 24105 Kiel, Germany.
authors.forename: Björn
authors.initials: B
authors.lastname: Möller
authors.affiliation: Department of Oral & Maxillofacial Surgery, Christian-Albrechts-University, Arnold-Heller-Str. 16, 24105 Kiel, Germany.
authors.forename: Volker
authors.initials: V
authors.lastname: Gassling
authors.affiliation: Department of Oral & Maxillofacial Surgery, Christian-Albrechts-University, Arnold-Heller-Str. 16, 24105 Kiel, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Wiltfang
authors.affiliation: Department of Oral & Maxillofacial Surgery, Christian-Albrechts-University, Arnold-Heller-Str. 16, 24105 Kiel, Germany. Electronic address: gierloff@mkg.uni-kiel.de.
authors.forename: Matthias
authors.initials: M
authors.lastname: Gierloff
mesh_terms: D050156:Adipogenesis / Q000502:physiology; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001973:Bromodeoxyuridine; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D020219:Chondrogenesis / Q000502:physiology; D003094:Collagen / Q000737:chemistry; D004396:Coloring Agents; D005456:Fluorescent Dyes; D006820:Hyaluronic Acid / Q000737:chemistry; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000502:physiology; D051381:Rats; D013234:Stem Cells / Q000502:physiology; D017154:Stromal Cells / Q000502:physiology*; D013778:Tetrazolium Salts; D013844:Thiazoles; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: C110656:2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium; C106416:Bio-Gide; D001672:Biocompatible Materials; D004396:Coloring Agents; D005456:Fluorescent Dyes; D008567:Membranes, Artificial; D013778:Tetrazolium Salts; D013844:Thiazoles; D006820:Hyaluronic Acid; D003094:Collagen; D007770:L-Lactate Dehydrogenase; C022616:thiazolyl blue; D001973:Bromodeoxyuridine
keywords: Adipose tissue derived stroma cells (ASC's); Biocompatibility; Collagen membranes; Hyaluronic acid gel; Multilineage potential; Scaffold; Tissue engineering