title: Trehalose metabolism in dormant and activated spores of Phycomyces blakesleeanus Burgeff.
abstract: Evidence is obtained for the existence of two different localizations of trehalase (α,α-trehalose glucohydrolase, EC 3.2.1.28) in Phycomyces spores: one inside the cell, and one in the periplasmic region. The latter enzyme is sensitive to 0.1 mol l(-1) HCl treatment and its activity can be regulated by external pH changes. The periplasmic form of the enzyme is involved in the metabolism of added labelled trehalose. This sugar is hydrolyzed externally to glucose which is found mainly in the incubation medium and which is partly absorbed by the spores. During incubation trehalose leaks out from both dormant and activated spores and is subsequently hydrolyzed to glucose. The intracellular trehalase is probably involved in the breakdown of endogenous trehalose in spores. After heat activation the hydrolysis of endogenous trehalose is stimulated even without an important increase in activity of intracellular trehalase. Additional treatments which break dormancy of spores without a significant activation of trehalase are the following: heating of HCl-treated spores and treatment of spores with reducing substances (e.g. Na2S2O4 and NaHSO3).
authors.affiliation: Laboratorium voor Plantenbiochemie, K.U. Leuven, Vaartstraat 24, B-3000, Leuven, Belgium.
authors.forename: J A
authors.initials: JA
authors.lastname: Van Assche
authors.forename: A J
authors.initials: AJ
authors.lastname: Van Laere
authors.forename: A R
authors.initials: AR
authors.lastname: Carlier