title: Effect of short-term enzymatic treatment on cell migration and cartilage regeneration: in vitro organ culture of bovine articular cartilage.
abstract: Depending on the damage extent and adjacent tissue condition in traumatic cartilage injury, it is possible to heal the tissue by resident cells. Unlike autologous chondrocyte implantation, short-term enzymatic treatment is an effective single-step procedure without extra cell expansion. Moreover, this method has been shown to significantly increase cellularity in lesion edges, resulting in enhanced integration and interfacial strength. We hypothesize that the locally digested extracellular matrix by treatment allows effortless cell migration from the adjacent tissue. Full-thickness cartilage discs and osteochondral explants were prepared from mature bovine stifle joints. These specimens were treated with collagenase in a culture medium. Two concentrations, 0.25 and 0.5 mg/mL, were used with various treating time of 10, 30, and 180 min. The cartilages were subsequently washed and cultured with fibrin hydrogel. The effect of enzymatic treatment on cell migration was apparent in both experiments of the cartilage disc and full-thickness cartilage defect model. In the disc culture, the treatment resulted in an approximately three to four times higher number of migrated cells than nontreated control. In short-term collagenase-treated groups, the proteoglycan (PG) loss was localized in the edge of tissue with minimal cell death. The treatment also accelerated cell migration in the full-thickness cartilage defects and some cells differentiated into chondrocytes with the deposit of PG. Gene expression results could support the characteristics of migrated cells, which had migratory ability and chondrogenic differentiation potential with overexpression of collagen type I and II, respectively. Based on these results, short-term enzymatic treatment, which can accelerate cell migration into traumatically injured cartilage, has great potential for clinical application.
authors.affiliation: 1 Department of Orthopaedics and Rehabilitation, The University of Iowa , Iowa City, Iowa.
authors.forename: Dongrim
authors.initials: D
authors.lastname: Seol
authors.forename: Yin
authors.initials: Y
authors.lastname: Yu
authors.forename: Hyeonghun
authors.initials: H
authors.lastname: Choe
authors.forename: Keewoong
authors.initials: K
authors.lastname: Jang
authors.forename: Marc J
authors.initials: MJ
authors.lastname: Brouillette
authors.forename: Hongjun
authors.initials: H
authors.lastname: Zheng
authors.forename: Tae-Hong
authors.initials: TH
authors.lastname: Lim
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Buckwalter
authors.forename: James A
authors.initials: JA
authors.lastname: Martin
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D002417:Cattle; D016923:Cell Death / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D047009:Hathewaya histolytica / Q000201:enzymology*; D017364:Collagenases / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D066298:In Vitro Techniques; D009924:Organ Culture Techniques; D011509:Proteoglycans / Q000378:metabolism; D012038:Regeneration / Q000187:drug effects*; D013997:Time Factors
chemical_list: D011509:Proteoglycans; D017364:Collagenases