title: Novel polysaccharide from Radix Cyathulae officinalis Kuan can improve immune response to ovalbumin in mice.
abstract: This study was designed to investigate the effects of oral administration of the polysaccharide from the Radix Cyathulae officinalis Kuan (RCPS) for its adjuvant potential on the specific cellular and humoral immune responses in mice. In this study, our data demonstrated that oral administration of RCPS significantly enhanced the phagocytic capacity of peritoneal macrophage, splenocyte proliferation, the activity of natural killer (NK) cells and cytotoxic T lymphocytes (CTL) and OVA-speciﬁc IgG, IgG1, IgG2a, and IgG2b antibody titers. Furthermore, RCPS promoted the level of interleukin-2(IL-2), IFN-γ and IL-4 in CD4(+)T cells and level of IFN-γ in CD8(+)T cells. In addition, RCPS enhanced the expression of CD40(+), CD80(+) and CD86(+) on the dendritic cells (DCs). Importantly, RCPS down-regulated the frequency of CD4(+)CD25(+)Foxp3(+)Treg cells. Taken together, these results suggested that RCPS could increase both cellular and humoral immune responses via up-regulating DCs maturation, and suppressing Treg frequency.
authors.affiliation: Isotope Research Laboratory, College of Life Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China; Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, PR China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Feng
authors.affiliation: Isotope Research Laboratory, College of Life Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Du
authors.affiliation: Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
authors.affiliation: Isotope Research Laboratory, College of Life Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.
authors.forename: Xingfa
authors.initials: X
authors.lastname: Han
authors.affiliation: Isotope Research Laboratory, College of Life Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Cao
authors.affiliation: Isotope Research Laboratory, College of Life Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China. Electronic address: xyzeng1966@163.com.
authors.forename: Xianyin
authors.initials: X
authors.lastname: Zeng
mesh_terms: D029044:Achyranthes / Q000737:chemistry*; D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D000284:Administration, Oral; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D020014:K562 Cells; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology; D051379:Mice; D010047:Ovalbumin / Q000276:immunology*; D010587:Phagocytosis / Q000187:drug effects; D011134:Polysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology
chemical_list: D000276:Adjuvants, Immunologic; D016207:Cytokines; D007074:Immunoglobulin G; D011134:Polysaccharides; D010047:Ovalbumin
keywords: Immune response; Polysaccharide; Radix Cyathula