title: Immunochemical quantification of cytochrome f in leaves of a non-yellowing senescence mutant of Festuca pratensis.
abstract: A non-competitive enzyme-linked immunosorbent assay (ELISA) which enables detection of as little as 0.1 ng cytochrome f in leaf extracts has been developed. No evidence for specific or non-specific interference by proteins other than cytochrome f was found. The assay was applied to a comparative study of age-related changes in the cytochrome f content of leaves of Festuca pratensis Huds. cv. Rossa, and a non-yellowing mutant genotype (Bf993) having a lesion in the mechanism responsible for thylakoid membrane disassembly. Cytochrome f in senescent leaves of the latter genotype was found to be present at significantly higher levels than in the wild-type, implying an inability on the part of the mutant to degrade this protein. The results obtained by ELISA were confirmed by antibody probing of Western blots.
authors.affiliation: Welsh Plant Breeding Station, Cell Biology Department, AFRC Institute for Grassland and Animal Production, Plas Gogerddan, SY23 3EB, Aberystwyth, Dyfed, UK.
authors.forename: T G
authors.initials: TG
authors.lastname: Davies
authors.forename: H
authors.initials: H
authors.lastname: Thomas
authors.forename: L J
authors.initials: LJ
authors.lastname: Rogers