title: High laccase producing mutants ofPleurotus florida.
abstract: Pleurotus florida produced high amounts of laccase (4.60 U/ml) in malt extract broth after 12 days' growth under stationary conditions. The production of laccase was semi-constitutive. Hyperlaccase mutants ofP. florida were obtained through mutagenesis of mycelial protoplasts usingN-methyl-N'-nitro-N-nitrosoguanidine (50 μg/ml) for 2 min. Three hyperlaccase mutants were selected showing growth and enzyme production responses similar to the parent.
authors.affiliation: Department of Microbiology, Punjab Agricultural University, 141 004, Ludhiana, India.
authors.forename: R P
authors.initials: RP
authors.lastname: Dhaliwal
authors.forename: H S
authors.initials: HS
authors.lastname: Garcha
authors.forename: P K
authors.initials: PK
authors.lastname: Khanna