title: Butyrate suppresses proliferation and migration of RKO colon cancer cells though regulating endocan expression by MAPK signaling pathway.
abstract: Butyrate is a short-chain fatty acid produced by colonic bacterial fermentation. In colon cancer cells butyrate is able to suppress cell growth, induce apoptosis. It also inhibits tumor growth in vivo. However, the underlying mechanism is still not fully understood. We hypothesize that butyrate regulates the growth and migration of colon cancer cells by altering endocan expression. To test this hypothesis, we performed quantitative real time RT–PCR and Western blots, and found that butyrate increased endocan expression of colon cancer cell RKO. Moreover, endocan over-expression inhibited RKO proliferation, migration and colony formation. Functionally, butyrate significantly suppressed RKO proliferation, migration, and colony formation, as well as induced apoptosis. Knocking down endogenous endocan was able to attenuate the inhibitory role of butyrate in RKO migration and proliferation. Since our results showed that butyrate inhibited MAPK/ERK2 phosphorylation. To determine whether ERK2 signaling is associated with endocan expression, we knocked down endogenous ERK2 expression. Our results showed that knocking down ERK2 expression up-regulated endocan expression. Taken together, these results suggested that butyrate suppressed RKO proliferation, colony formation, migration through up-regulating endocan expression via ERK2/MAPK signaling pathway.
authors.forename: Li
authors.initials: L
authors.lastname: Zuo
authors.forename: Man
authors.initials: M
authors.lastname: Lu
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhou
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
mesh_terms: D002087:Butyrates / Q000494:pharmacology*; D045744:Cell Line, Tumor / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D019950:Mitogen-Activated Protein Kinase 1 / Q000235:genetics / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D011509:Proteoglycans / Q000235:genetics / Q000378:metabolism*
chemical_list: D002087:Butyrates; C101134:ESM1 protein, human; D009363:Neoplasm Proteins; D011509:Proteoglycans; C535150:MAPK1 protein, human; D019950:Mitogen-Activated Protein Kinase 1