title: Development and validation of RP-HPLC method for quantification of glipizide in biological macromolecules.
abstract: Glipizide (GPZ) has been widely used in the treatment of type-2 diabetics as insulin secretogague. Multiunit chitosan based GPZ floating microspheres was prepared by ionotropic gelation method for gastroretentive delivery using sodiumtripolyphosphate as cross-linking agent. Pharmacokinetic study of microspheres was done in rabbit and plasma samples were analyzed by a newly developed and validated high-performance liquid chromatographic method. Method was developed on Hypersil ODS-18 column using a mobile phase of 10mM phosphate buffer (pH, 3.5) and methanol (25:75, v/v). Elute was monitored at 230 nm with a flow rate of 1 mL/min. Calibration curve was linear over the concentration range of 25.38-2046.45 ng/mL. Retention times of GPZ and internal standard (gliclazide) were 7.32 and 9.02 min respectively. Maximum plasma drug concentration, area under the plasma drug concentration-time curve and elimination half life for GPZ floating microspheres were 2.88±0.29 μg mL(-1), 38.46±2.26 μg h mL(-1) and 13.55±1.36 h respectively. When the fraction of drug dissolved from microspheres in pH 7.4 was plotted against the fraction of drug absorbed, a linear correlation (R(2)=0.991) was obtained in in vitro and in vivo correlation study.
authors.affiliation: Department of Pharmaceutics, Gayatri College of Pharmacy, Sambalpur, Odisha, India. Electronic address: niharpani@gmail.com.
authors.forename: Nihar Ranjan
authors.initials: NR
authors.lastname: Pani
authors.affiliation: Department of Pharmaceutical Sciences, Utkal University, Bhubaneswar, Odisha, India.
authors.forename: Sujata
authors.initials: S
authors.lastname: Acharya
authors.affiliation: Department of Pharmaceutical Sciences, Utkal University, Bhubaneswar, Odisha, India.
authors.forename: Sradhanjali
authors.initials: S
authors.lastname: Patra
mesh_terms: D000818:Animals; D001774:Blood Chemical Analysis / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D003692:Delayed-Action Preparations; D005913:Glipizide / Q000097:blood* / Q000493:pharmacokinetics; D016014:Linear Models; D008863:Microspheres; D011817:Rabbits; D013997:Time Factors
chemical_list: D003692:Delayed-Action Preparations; D005913:Glipizide
keywords: Floating microspheres; HPLC; Pharmacokinetics; glipizide; rabbit