title: Nanoscopy of filamentous actin in cortical dendrites of a living mouse.
abstract: We demonstrate superresolution fluorescence microscopy (nanoscopy) of protein distributions in a mammalian brain in vivo. Stimulated emission depletion microscopy reveals the morphology of the filamentous actin in dendritic spines down to 40 μm in the molecular layer of the visual cortex of an anesthetized mouse. Consecutive recordings at 43-70 nm resolution reveal dynamical changes in spine morphology.
authors.affiliation: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany; Center for Nanoscale Microscopy and Molecular Physiology of the Brain, Göttingen, Germany. Electronic address: kwillig@gwdg.de.
authors.forename: Katrin I
authors.initials: KI
authors.lastname: Willig
authors.affiliation: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
authors.forename: Heinz
authors.initials: H
authors.lastname: Steffens
authors.affiliation: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
authors.forename: Carola
authors.initials: C
authors.lastname: Gregor
authors.affiliation: Department of Molecular Neurobiology and Department of Psychiatry, Ludwig-Maximilians-University, München, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Herholt
authors.affiliation: Department of Molecular Neurobiology and Department of Psychiatry, Ludwig-Maximilians-University, München, Germany.
authors.forename: Moritz J
authors.initials: MJ
authors.lastname: Rossner
authors.affiliation: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany; Center for Nanoscale Microscopy and Molecular Physiology of the Brain, Göttingen, Germany. Electronic address: shell@gwdg.de.
authors.forename: Stefan W
authors.initials: SW
authors.lastname: Hell
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure*; D000818:Animals; D003712:Dendrites / Q000378:metabolism / Q000648:ultrastructure*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D014793:Visual Cortex / Q000378:metabolism / Q000648:ultrastructure*