title: Effect of heat-killed Lactobacillus brevis SBC8803 on cutaneous arterial sympathetic nerve activity, cutaneous blood flow and transepidermal water loss in rats.
abstract: OBJECTIVE
  To evaluate the efficacy of the effects of heat-killed Lactobacillus brevis SBC8803 (HK-SBC8803) on the standard physiological markers of skin health of cutaneous arterial sympathetic nerve activity (CASNA), cutaneous blood flow and transepidermal water loss (TEWL) and to determine whether SBC8803 targets serotonin 5-HT3 receptors in rats.
  
  
  RESULTS
  A set of three experiments were conducted to examine the effects of SBC8803 on CASNA, cutaneous blood flow and TEWL using Wistar and hairless rats. Two additional experiments further attempted to determine whether HK-SBC8803 was targeting the serotonin 5-HT3 receptors by pretreatment with the 5-HT3 antagonist granisetron. Administration of HK-SBC8803 in the first three experiments caused marked inhibition of CASNA and significant elevation of cutaneous blood flow under urethane anaesthesia as well as significant decrease in TEWL on the dorsal skin of conscious hairless rats. Pretreatment with granisetron decreased the effects of HK-SBC8803 on CASNA and cutaneous blood flow.
  
  
  CONCLUSIONS
  These findings suggest that HK-SBC8803 reduces CASNA, increases cutaneous blood flow and decreases TEWL and that 5-HT3 receptors may be involved in CASNA and cutaneous blood flow responses.
  
  
  CONCLUSIONS
  HK-SBC8803 could be a useful substance in the treatment/prevention of skin problems, specifically chapped or dry skin.
authors.affiliation: ANBAS Corporation, Osaka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Horii
authors.forename: H
authors.initials: H
authors.lastname: Kaneda
authors.forename: Y
authors.initials: Y
authors.lastname: Fujisaki
authors.forename: R
authors.initials: R
authors.lastname: Fuyuki
authors.forename: Y
authors.initials: Y
authors.lastname: Nakakita
authors.forename: T
authors.initials: T
authors.lastname: Shigyo
authors.forename: K
authors.initials: K
authors.lastname: Nagai
mesh_terms: D000818:Animals; D001158:Arteries / Q000294:innervation; D017829:Granisetron / Q000494:pharmacology; D006358:Hot Temperature; D052196:Levilactobacillus brevis; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D044406:Receptors, Serotonin, 5-HT3; D012039:Regional Blood Flow; D012702:Serotonin Antagonists / Q000494:pharmacology; D012867:Skin / Q000098:blood supply*; D013564:Sympathetic Nervous System / Q000502:physiology*; D014870:Water Loss, Insensible
chemical_list: D017829:Granisetron; D044406:Receptors, Serotonin, 5-HT3; D012702:Serotonin Antagonists
keywords: 5-HT3 receptor; Lactobacillus brevis; cutaneous arterial sympathetic nerve activity; cutaneous blood flow; transepidermal water loss