title: Circulation of group A rotaviruses among neonates of human, cow and pig: study from Assam, a north eastern state of India.
abstract: Rotavirus (RV) infections are worldwide in distribution causing high morbidity and mortality in human and animal neonates. Human settlements in close proximity of animals aids for genetic re-assortment of the virus by interspecies transmission and consequent emergence of new viral antigenic strain. Therefore, the present study was designed to explore RV incidence in a single approach from human and animal neonates sharing similar environment. Altogether, 200 diarrheal samples from children (50), piglets (80) and calves (70) were collected during the year of 2010-2012 from various locality, farms and hospitals, initially screened through monoclonal antibody based enzyme immunoassay followed by RNA-PAGE and VP7 gene amplification by Reverse transcription PCR. The overall prevalence of rotavirus was found to be 41.5 % (83/200) where maximum numbers of positive cases were found in piglets (46.3 %) followed by human (40 %) and cow (37.1 %). Majority of samples demonstrated characteristic group A rotavirus (RVA) electropherotype of 4:2:3:2 pattern. Moreover, RNA profiles of seven samples from piglets and calves revealed variation in the migration pattern of class II, III and class IV segments. The study, for the first time from the valley, detected 43.7 % of neonatal RVA positive cases from human and animal sharing similar setting. The variation in RNA migration pattern in seven cases signifies tentative cases of gene re-assortment that warrant further evaluation.
authors.affiliation: Department of Microbiology, College of Veterinary Sciences, Assam Agricultural University, Khanapara, Guwahati, 781022 Assam India.
authors.forename: Rinky
authors.initials: R
authors.lastname: Sharma
authors.affiliation: Department of Microbiology, College of Veterinary Sciences, Assam Agricultural University, Khanapara, Guwahati, 781022 Assam India.
authors.forename: Durlav Prasad
authors.initials: DP
authors.lastname: Bora
authors.affiliation: Department of Microbiology, College of Veterinary Sciences, Assam Agricultural University, Khanapara, Guwahati, 781022 Assam India.
authors.forename: Paromita
authors.initials: P
authors.lastname: Chakraborty
authors.affiliation: Department of Microbiology, College of Veterinary Sciences, Assam Agricultural University, Khanapara, Guwahati, 781022 Assam India.
authors.forename: Sushmita
authors.initials: S
authors.lastname: Das
authors.affiliation: Department of Microbiology, College of Veterinary Sciences, Assam Agricultural University, Khanapara, Guwahati, 781022 Assam India.
authors.forename: Nagendra Nath
authors.initials: NN
authors.lastname: Barman
keywords: Electropherotype; Neonates; Reassortment; Rotavirus; VP7