title: Silica-coated dimers of silver nanospheres as surface-enhanced Raman scattering tags for imaging cancer cells.
abstract: Surface-enhanced Raman scattering (SERS) tags have been actively explored as a multiplexing platform for sensitive detection of biomolecules. Here, we report a new type of SERS tags that was fabricated by sequentially functionalizing dimers made of 50 nm Ag nanospheres with 4-mercaptobenzoic acid as the Raman reporter molecule, silica coating as a protective shell and antibody as a targeting ligand. These dimer-based tags give highly enhanced and reproducible Raman signals owing to the presence of a well-defined SERS hot spot at the junction between two Ag nanospheres in the dimer. The SERS enhancement factor (EF) of an individual dimer tag supported on a glass slide can reach a level as high as 4.3 × 10(6). In comparison, the EFs dropped to 2.8 × 10(5) and 8.7 × 10(5), respectively, when Ag nanospheres and nanocubes with sizes similar to the spheres in the dimer were used to fabricate the tags using similar procedures. The SERS signals from aqueous suspensions of the dimer-based tags also showed high intensity and good stability. Potential use of the dimer-based tags was demonstrated by imaging cancer cells overexpressing HER2 receptors with good specificity and high sensitivity.
authors.affiliation: The Wallace H. Coulter Department of Biomedical Engineering , Georgia Institute of Technology and Emory University Medical School ; School of Chemistry and Biochemistry, School of Chemical and Biomolecular Engineering , Georgia Institute of Technology , Atlanta, GA 30332 , USA.
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Xia
authors.affiliation: Department of Materials Science and Engineering , Stanford University , Stanford, CA 94305 , USA.
authors.forename: Weiyang
authors.initials: W
authors.lastname: Li
authors.affiliation: The Wallace H. Coulter Department of Biomedical Engineering , Georgia Institute of Technology and Emory University Medical School ; School of Chemistry and Biochemistry, School of Chemical and Biomolecular Engineering , Georgia Institute of Technology , Atlanta, GA 30332 , USA.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: The Wallace H. Coulter Department of Biomedical Engineering , Georgia Institute of Technology and Emory University Medical School ; School of Chemistry and Biochemistry, School of Chemical and Biomolecular Engineering , Georgia Institute of Technology , Atlanta, GA 30332 , USA.
authors.forename: Younan
authors.initials: Y
authors.lastname: Xia
keywords: cancer cell; dimer; hot spot; imaging; surface-enhanced Raman scattering; surface-enhanced Raman scattering tag