title: Superoxide dismutase activity in the oviductal and uterine fluid of the bitch and the effects of the enzyme on viability, motility and hyperactivation of canine sperm in vitro.
abstract: Superoxide dismutase (SOD) activity in flushings from oviducts and uterine horns of 8 anestrous, 5 estrous and 7 diestrous bitches was measured. SOD activity in oviductal fluid in estrous bitches was significantly higher than that in anestrous and diestrous bitches (P<0.01). SOD activity in uterine fluid of diestrous bitches was, however, significantly higher than that in anestrous and estrous bitches (P<0.01). Additionally, sperm collected from normal dogs were incubated in MEM and in MEM containing SOD (SOD-MEM) for 24 hr. The percentages of sperm with viability, motility and hyperactivation in SOD-MEM were higher than those in MEM. SOD produced in oviduct and uterus may be able to maintain or improve sperm quality and fertility in the dog.
authors.affiliation: Laboratory of Reproduction, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino-shi, Tokyo 180-8602, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Miho
authors.initials: M
authors.lastname: Wada
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Hori
authors.forename: Eiichi
authors.initials: E
authors.lastname: Kawakami
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004285:Dogs; D030762:Estrous Cycle / Q000378:metabolism; D045604:Extracellular Fluid / Q000201:enzymology*; D005260:Female; D008297:Male; D010057:Oviducts / Q000201:enzymology*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism* / Q000494:pharmacology; D014599:Uterus / Q000201:enzymology*
chemical_list: D013482:Superoxide Dismutase