title: Isolation and characterization of restriction endonuclease EclHKI from an Enterobacter cloacae strain.
abstract: An Enterobacter cloacae strain, producing restriction enzyme EclHKI, was isolated from a decaying potato. The enzyme is an isoschizomer of Eam1105I, which recognizes and cleaves GACNNN!NNGTC. EclHKI was produced at high activity (4×10(4) U/g wet cells) and was purified from contaminants which interfere with restriction digestion by passing the cell lysate through DEAE-Sephacel and heparin columns. Activity was optimal at 37°C in a medium salt buffer.
authors.affiliation: , .
authors.forename: H Y
authors.initials: HY
authors.lastname: Chan
authors.forename: Y C
authors.initials: YC
authors.lastname: Chan
authors.forename: K M
authors.initials: KM
authors.lastname: Kam
authors.forename: P C
authors.initials: PC
authors.lastname: Shaw