title: Performance of SaSelect, a chromogenic medium for detection of staphylococci in clinical specimens.
abstract: In a preliminary study, known staphylococcus (n = 86) and other microbial (n = 12) isolates were plated on three chromogenic media, SaSelect (Bio-Rad, Hercules, CA, USA), CHROMagar Staph. aureus (CHROMagar Microbiology, Paris, France), and S. aureus ID (bioMérieux, Marcy l'Etoile, France). The sensitivities of all the media to detect Staphylococcus aureus after 24 h of incubation were high (100.0%). However, their specificities varied at 93.3% (95% confidence interval [CI], 86.0% to 100.0%) (CHROMagar Staph. aureus), 97.8% (95% CI, 93.5% to 100.0%) (S. aureus ID), and 100.0% (SaSelect). SaSelect also showed the highest sensitivity for recovery and differentiation of other staphylococci. As the best performing chromogenic medium, SaSelect was then prospectively compared to conventional culture and identification tests for the detection of staphylococci from 2,780 clinical specimens. A total of 1,589 staphylococcal isolates were recovered. Of these, 912 were S. aureus and 677 were other staphylococci. The sensitivity and specificity of SaSelect to detect S. aureus in clinical specimens after 24 h of incubation were 99.6% and 99.9% (95% CI, 99.2% to 100.0% and 99.8% to 100.0%), respectively, whereas the sensitivity and specificity using conventional plates combined with laboratory identification methods were 96.8% and 99.5% (95% CI, 95.7 to 97.9% and 99.2% to 99.8%). For the recovery and preliminary identification of other staphylococci, the sensitivity and specificity of SaSelect were 94.4% and 99.9%. SaSelect is a well-performing chromogenic medium that significantly improved the detection of staphylococci, especially S. aureus, compared to conventional culture (P < 0.0001).
authors.affiliation: Department of Clinical Microbiology, Vaasa Central Hospital, Vaasa, Finland.
authors.forename: Jari J
authors.initials: JJ
authors.lastname: Hirvonen
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Kerttula
authors.forename: Suvi-Sirkku
authors.initials: SS
authors.lastname: Kaukoranta
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D002863:Chromogenic Compounds / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry*; D006801:Humans; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis*; D013210:Staphylococcus / Q000302:isolation & purification*; D013997:Time Factors
chemical_list: D002863:Chromogenic Compounds; D003470:Culture Media