title: Detection of single-digit foodborne pathogens with the naked eye using carbon nanotube-based multiple cycle signal amplification.
abstract: A carbon nanotube (CNT)-based multiple cycle signal amplification strategy has been demonstrated for detection of single-digit foodborne pathogens with the naked eye. In the present design, CNTs are used as carriers for loading numerous horseradish peroxidase (HRP) and concanavalin A (ConA) tags, and multiple cycle signal amplification is achieved through the biotinylated anti-HRP antibody and avidin-HRP.
authors.affiliation: Key Laboratory of Sensing Technology and Biomedical Instruments (Guangdong Province), School of Engineering, Sun Yat-Sen University, Guangzhou 510275, China. yichq@mail.sysu.edu.cn.
authors.forename: Heng
authors.initials: H
authors.lastname: Zhang
authors.forename: Yupeng
authors.initials: Y
authors.lastname: Shi
authors.forename: Fang
authors.initials: F
authors.lastname: Lan
authors.forename: Yi
authors.initials: Y
authors.lastname: Pan
authors.forename: Yankui
authors.initials: Y
authors.lastname: Lin
authors.forename: Jingzhang
authors.initials: J
authors.lastname: Lv
authors.forename: Zhihao
authors.initials: Z
authors.lastname: Zhu
authors.forename: Qing
authors.initials: Q
authors.lastname: Jiang
authors.forename: Changqing
authors.initials: C
authors.lastname: Yi
mesh_terms: D001360:Avidin / Q000737:chemistry / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D003208:Concanavalin A / Q000737:chemistry / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005516:Food Microbiology; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D057230:Limit of Detection; D008460:Meat / Q000382:microbiology; D037742:Nanotubes, Carbon / Q000737:chemistry*; D012475:Salmonella / Q000302:isolation & purification*
chemical_list: D037742:Nanotubes, Carbon; D003208:Concanavalin A; D001360:Avidin; D006735:Horseradish Peroxidase