title: Purification, crystallization and preliminary X-ray diffraction of the N-terminal calmodulin-like domain of the human mitochondrial ATP-Mg/Pi carrier SCaMC1.
abstract: SCaMC is an ATP-Mg/Pi carrier protein located at the mitochondrial inner membrane. SCaMC has an unusual N-terminal Ca(2+)-binding domain (NTD) in addition to its characteristic six-helix transmembrane bundle. The NTD of human SCaMC1 (residues 1-193) was expressed and purified in order to study its role in Ca(2+)-regulated ATP-Mg/Pi transport mediated by its transmembrane domain. While Ca(2+)-bound NTD could be crystallized, the apo state resisted extensive crystallization trials. Selenomethionine-labeled Ca(2+)-bound NTD crystals, which belonged to space group P6(2)22 with one molecule per asymmetric unit, diffracted X-rays to 2.9 Å resolution.
authors.affiliation: Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Qin
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Sven
authors.initials: S
authors.lastname: Brüschweiler
authors.affiliation: Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: James J
authors.initials: JJ
authors.lastname: Chou
mesh_terms: D017920:Antiporters / Q000737:chemistry* / Q000302:isolation & purification*; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D002147:Calmodulin / Q000737:chemistry*; D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D024101:Mitochondrial Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D017434:Protein Structure, Tertiary; D014961:X-Ray Diffraction
chemical_list: C086357:ATP-Mg-Pi carrier proteins, mitochondria; D017920:Antiporters; D002135:Calcium-Binding Proteins; D002147:Calmodulin; D024101:Mitochondrial Proteins; C487500:SLC25A24 protein, human; D002118:Calcium
keywords: SCaMC; SLC25A24; calcium-sensing domain; conformation switch; mitochondrial ATP-Mg transporter