title: [Effects of different salt-alkaline stress on seed germination and seedling physiological characteristics of Codonopsis pilosula].
abstract: OBJECTIVE
  To improve salt resistance of Codonopsis pilosula seeds and seedlings under different salt-alkaline stress, the seed germination and physiological characteristics of seedlings were studied.
  
  
  METHODS
  Several physiological indexes of Codonopsis pilosula under different salt-alkaline stress were measured, such as the germination vigor, germination rate, relative germination rate and recovery germination rate,as well as others indexes like the contents of chlorophyll, soluble protein, water and MDA and the activities of POD and SOD.
  
  
  RESULTS
  The inhibitory effects of three salts on seed germination were Na2 CO3 > NaCl + Na2 CO3 > NaCl with an increased salt concentration. The contents of chlorophyll, soluble protein and water were found significant different (P < 0.05) from the negative control group, along with the decreased of these indexes. However, positive correlation existed between MDA content and salt treatment concentration. In addition, the change trend of SOD and POD activity were similar. They were increased firstly, and then decreased as the stress intensity extended.
  
  
  CONCLUSIONS
  The seeds and seedlings under different salt-alkaline stress were inhibited, with different inhibitory extent as Na2CO3 > NaCl + Na2 CO3 > NaCl.
authors.affiliation: College of Agriculture and Biotechnology, Hexi University, Zhangye 734000, China. handuohong@163.com
authors.forename: Duo-Hong
authors.initials: DH
authors.lastname: Han
authors.affiliation: College of Agriculture and Biotechnology, Hexi University, Zhangye 734000, China.
authors.forename: Xiu-Fang
authors.initials: XF
authors.lastname: Zheng
authors.affiliation: College of Agriculture and Biotechnology, Hexi University, Zhangye 734000, China.
authors.forename: En-Jun
authors.initials: EJ
authors.lastname: Wang
authors.affiliation: Gansu College of Traditional Chinese Medicine, Lanzhou 730000, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Jin
authors.affiliation: College of Agriculture and Biotechnology, Hexi University, Zhangye 734000, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Agriculture and Biotechnology, Hexi University, Zhangye 734000, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Dai
mesh_terms: D000468:Alkalies / Q000378:metabolism*; D002734:Chlorophyll / Q000378:metabolism; D029745:Codonopsis / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology; D018525:Germination / Q000502:physiology; D036226:Seedlings / Q000254:growth & development / Q000378:metabolism / Q000502:physiology; D012639:Seeds / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology; D012965:Sodium Chloride / Q000378:metabolism*; D013312:Stress, Physiological; D013431:Sulfates / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000468:Alkalies; D013431:Sulfates; D002734:Chlorophyll; D012965:Sodium Chloride; D013482:Superoxide Dismutase