title: Phytoextracts-Synthesized Silver Nanoparticles Inhibit Bacterial Fish Pathogen Aeromonas hydrophila.
abstract: Fish disease is a major stumbling block towards sustainable growth of the fisheries sector. Aeromonas hydrophila, which is a major infectious aquatic pathogen is reportedly the causative agent of ulcers, fin-rot, tail-rot, hemorrhagic septicemia in fish, and has reportedly developed resistance against many of the available antibiotics. In this context, the inhibitory function of silver nanoparticles (AgNPs) against A. hydrophila was studied to evaluate its possible application in aquaculture as alternative to antibiotics. AgNPs were synthesized using the leaf extracts of subtropical plants Mangifera indica (Mango), Eucalyptus terticornis (Eucalyptus), Carica papaya (Papaya) and Musa paradisiaca (Banana). The absorbance maxima, size range and shape of the AgNPs as characterized by the UV-Vis spectroscopy, high resolution transmission electron microscopy (HR-TEM), and energy dispersive X-ray spectroscopy (EDX) were, Mangifera-442, 50-65 nm, ovular; Eucalyptus-465, 60-150 nm, oval; Carica-442, 25-40 nm, round, irregular; and Musa-454, 10-50 nm, round, irregular, respectively. Well-diffusion of these AgNPs for their antimicrobial characteristics exhibited that, the papaya leaf extract synthesized AgNPs had maximum antimicrobial activity at 153.6 μg/ml concentrations, and that from the eucalyptus leaves was least effective. As observed, the potency of the nanoparticles enhanced with the decrease in particle size, from 60-150 nm in eucalyptus to 25-40 nm in papaya. Due to its purely natural sourcing, phytosynthesized AgNPs can be applied as alternative to antibiotics and other biocides as a cost-effective and eco-friendly therapeutic agent against A. hydrophila stimulated diseases in aquatic animals.
authors.affiliation: Central Inland Fisheries Research Institute, Barrackpore, Kolkata, West Bengal India ; School of Biotechnology, KIIT University, Bhubaneswar, Odisha India.
authors.forename: Arabinda
authors.initials: A
authors.lastname: Mahanty
authors.affiliation: School of Biotechnology, KIIT University, Bhubaneswar, Odisha India.
authors.forename: Snehasish
authors.initials: S
authors.lastname: Mishra
authors.affiliation: Central Research Facility, Indian Institute of Technology, Kharagpur, West Bengal India.
authors.forename: Ranadhir
authors.initials: R
authors.lastname: Bosu
authors.affiliation: National Institute Abiotic Stress Management, Baramati, Pune, Maharashtra India.
authors.forename: Uk
authors.initials: U
authors.lastname: Maurya
authors.affiliation: School of Biotechnology, KIIT University, Bhubaneswar, Odisha India.
authors.forename: Surya Prakash
authors.initials: SP
authors.lastname: Netam
authors.affiliation: School of Biotechnology, KIIT University, Bhubaneswar, Odisha India ; National Institute Abiotic Stress Management, Baramati, Pune, Maharashtra India.
authors.forename: Biplab
authors.initials: B
authors.lastname: Sarkar
keywords: Aeromonas hydrophila; Anti-microbial activity; Phytosynthesis; Silver nanoparticle