title: Rift Valley fever virus infection induces activation of the NLRP3 inflammasome.
abstract: Inflammasome activation is gaining recognition as an important mechanism for protection during viral infection. Here, we investigate whether Rift Valley fever virus, a negative-strand RNA virus, can induce inflammasome responses and IL-1β processing in immune cells. We have determined that RVFV induces NLRP3 inflammasome activation in murine dendritic cells, and that this process is dependent upon ASC and caspase-1. Furthermore, absence of the cellular RNA helicase adaptor protein MAVS/IPS-1 significantly reduces extracellular IL-1β during infection. Finally, direct imaging using confocal microscopy shows that the MAVS protein co-localizes with NLRP3 in the cytoplasm of RVFV infected cells.
authors.affiliation: Center for Global Health and Diseases, Case Western Reserve University, Cleveland, OH 44106, USA; Department of Pathology, Case Western Reserve University, Cleveland, OH, USA.
authors.forename: Megan E
authors.initials: ME
authors.lastname: Ermler
authors.affiliation: Center for Global Health and Diseases, Case Western Reserve University, Cleveland, OH 44106, USA.
authors.forename: Zachary
authors.initials: Z
authors.lastname: Traylor
authors.affiliation: Center for Global Health and Diseases, Case Western Reserve University, Cleveland, OH 44106, USA.
authors.forename: Krupen
authors.initials: K
authors.lastname: Patel
authors.affiliation: Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, MA, USA.
authors.forename: Stefan A
authors.initials: SA
authors.lastname: Schattgen
authors.affiliation: Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, MA, USA.
authors.forename: Sivapriya K
authors.initials: SK
authors.lastname: Vanaja
authors.affiliation: Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, MA, USA.
authors.forename: Katherine A
authors.initials: KA
authors.lastname: Fitzgerald
authors.affiliation: Center for Global Health and Diseases, Case Western Reserve University, Cleveland, OH 44106, USA; Department of Pathology, Case Western Reserve University, Cleveland, OH, USA; Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, OH, USA. Electronic address: amy.hise@case.edu.
authors.forename: Amy G
authors.initials: AG
authors.lastname: Hise
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000276:immunology; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000276:immunology*; D020170:Caspase 1 / Q000235:genetics / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology / Q000821:virology; D005260:Female; D006801:Humans; D058847:Inflammasomes / Q000235:genetics / Q000276:immunology*; D053583:Interleukin-1beta / Q000235:genetics / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D012295:Rift Valley Fever / Q000235:genetics / Q000276:immunology* / Q000821:virology; D012296:Rift Valley fever virus / Q000235:genetics / Q000502:physiology*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D020170:Caspase 1; D058847:Inflammasomes; D053583:Interleukin-1beta; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C512612:MAVS protein, mouse; C492716:Nlrp3 protein, mouse
keywords: ASC; Caspase-1; Dendritic cells; IL-1β; Inflammasome; Murine; NLRP3; Rift Valley fever virus; Virus