title: Association of RAGE gene polymorphism with vascular complications in Indian type 2 diabetes mellitus patients.
abstract: OBJECTIVE
  The study was designed to evaluate the association of -374T/A and -429T/C polymorphism in the promoter region and Gly82Ser polymorphism in exon 3 region of RAGE gene with diabetic vascular complications in Indian population.
  
  
  METHODS
  We screened 603 subjects which includes 176 healthy controls, 140 type 2 diabetes mellitus (T2DM) subjects without any vascular complications (DM), 152 T2DM subjects with microvascular complications (DM-micro) and 135 T2DM subjects with macrovascular complications (DM-macro) for -374T/A, -429T/C and Gly82Ser polymorphisms of RAGE gene. DNA isolated from the enrolled subjects were genotyped by PCR-RFLP. Logistic regression analysis was used to evaluate the association of single nucleotide polymorphisms (SNPs).
  
  
  RESULTS
  The -429 T/C and Gly82Ser RAGE polymorphisms were found to be significantly associated with the development of macrovascular and microvascular complications, respectively, in T2DM subjects while -374A allele showed reduced risk towards the development of macrovascular complications. Further, -429T/C, -374T/A and Gly82Ser haplotype analysis revealed association of CTG haplotype with development of macrovascular complications while haplotype TAG was observed to be significantly protective towards development of macrovascular complications in T2DM subjects (OR=0.617, p=0.0202).
  
  
  CONCLUSIONS
  Our data indicates significant association of RAGE SNPs and haplotypes with vascular complications in North Indian T2DM subjects.
authors.affiliation: Biochemistry and Immunology Laboratory, Department of Biochemistry, University College of Medical Sciences (University of Delhi) and G.T.B. Hospital, Dilshad Garden, Delhi 110095, India. Electronic address: aktripathiucms@gmail.com.
authors.forename: Ashok Kumar
authors.initials: AK
authors.lastname: Tripathi
authors.affiliation: Biochemistry and Immunology Laboratory, Department of Biochemistry, University College of Medical Sciences (University of Delhi) and G.T.B. Hospital, Dilshad Garden, Delhi 110095, India. Electronic address: diweshchawla@yahoo.co.in.
authors.forename: Diwesh
authors.initials: D
authors.lastname: Chawla
authors.affiliation: Biochemistry and Immunology Laboratory, Department of Biochemistry, University College of Medical Sciences (University of Delhi) and G.T.B. Hospital, Dilshad Garden, Delhi 110095, India. Electronic address: bansalsavita_1916@yahoo.com.
authors.forename: Savita
authors.initials: S
authors.lastname: Bansal
authors.affiliation: Biochemistry and Immunology Laboratory, Department of Biochemistry, University College of Medical Sciences (University of Delhi) and G.T.B. Hospital, Dilshad Garden, Delhi 110095, India. Electronic address: banerjeebd@hotmail.com.
authors.forename: Basu Dev
authors.initials: BD
authors.lastname: Banerjee
authors.affiliation: Department of Medicine, University College of Medical Sciences (University of Delhi) and G.T.B. Hospital, Dilshad Garden, Delhi 110095, India. Electronic address: drsvmadhu@gmail.com.
authors.forename: Sri Venkata
authors.initials: SV
authors.lastname: Madhu
authors.affiliation: Department of Medicine, University College of Medical Sciences (University of Delhi) and G.T.B. Hospital, Dilshad Garden, Delhi 110095, India. Electronic address: opkalra1@yahoo.com.
authors.forename: Om Prakash
authors.initials: OP
authors.lastname: Kalra
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000503:physiopathology*; D003925:Diabetic Angiopathies / Q000235:genetics*; D005260:Female; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic / Q000235:genetics*; D000067759:Receptor for Advanced Glycation End Products / Q000235:genetics*
chemical_list: D000067759:Receptor for Advanced Glycation End Products
keywords: RAGE gene Polymorphism; Type 2 diabetes mellitus; Vascular complications