title: The novel steroidal alkaloids dendrogenin A and B promote proliferation of adult neural stem cells.
abstract: Dendrogenin A (DDA) and dendrogenin B (DDB) are new aminoalkyl oxysterols which display re-differentiation of tumor cells of neuronal origin at nanomolar concentrations. We analyzed the influence of dendrogenins on adult mice neural stem cell proliferation, sphere formation and differentiation. DDA and DDB were found to have potent proliferative effects in neural stem cells. Additionally, they induce neuronal outgrowth from neurospheres during in vitro cultivation. Taken together, our results demonstrate a novel role for dendrogenins A and B in neural stem cell proliferation and differentiation which further increases their likely importance to compensate for neuronal cell loss in the brain.
authors.affiliation: Department of Neuroscience, Karolinska Institute, Stockholm, Sweden. Electronic address: shaden.khalifa@ki.se.
authors.forename: Shaden A M
authors.initials: SA
authors.lastname: Khalifa
authors.affiliation: Affichem, Toulouse, France; INSERM UMR 1037, Team "Sterol Metabolism and Therapeutic Innovations in Oncology", Cancer Research Center of Toulouse, F-31052 Toulouse, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: de Medina
authors.affiliation: Department of Public Health and Caring Sciences, Uppsala University, Uppsala, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Erlandsson
authors.affiliation: Department of Medicinal Chemistry, Biomedical Centre, Uppsala University, Uppsala, Sweden.
authors.forename: Hesham R
authors.initials: HR
authors.lastname: El-Seedi
authors.affiliation: INSERM UMR 1037, Team "Sterol Metabolism and Therapeutic Innovations in Oncology", Cancer Research Center of Toulouse, F-31052 Toulouse, France; University of Toulouse III, Toulouse, France; Institut Claudius Regaud, Toulouse, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Silvente-Poirot
authors.affiliation: INSERM UMR 1037, Team "Sterol Metabolism and Therapeutic Innovations in Oncology", Cancer Research Center of Toulouse, F-31052 Toulouse, France; University of Toulouse III, Toulouse, France; Institut Claudius Regaud, Toulouse, France. Electronic address: marc.poirot@inserm.fr.
authors.forename: Marc
authors.initials: M
authors.lastname: Poirot
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002777:Cholestanols / Q000494:pharmacology*; D005260:Female; D007093:Imidazoles / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D058953:Neural Stem Cells / Q000187:drug effects*; D013095:Spermidine / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: C582666:5-hydroxy-6-(2-(1H-imidazol-4-yl)ethylamino)cholestan-3-ol; C582667:5-hydroxy-6-(3-(4-aminobutylamino)propylamino)cholest-7-en-3-ol; D002777:Cholestanols; D007093:Imidazoles; D013095:Spermidine
keywords: Dendrogenin; Neural stem cells; Neurosteroids; Oxysterols