title: Thiol-ene click chemistry derived cationic cyclodextrin chiral stationary phase and its enhanced separation performance in liquid chromatography.
abstract: This work is the first demonstration of a simple thiol-ene click chemistry to anchor vinyl imidazolium β-CD onto thiol silica to form a novel cationic native cyclodextrin (CD) chiral stationary phase (CSP). The CSP afforded high enantioseparation ability towards dansyl (Dns) amino acids, carboxylic aryl compounds and flavonoids in chiral HPLC. The current CSP demonstrates the highest resolving ability (selectivity >1.1, resolution >1.5) towards Dns amino acids in a mobile phase buffered at pH=6.5, with the resolution of Dns-dl-leucine as high as 6.97. 2,4-dichloride propionic acid (2,4-ClPOPA) was well resolved with the selectivity and resolution of 1.37 and 4.88, respectively. Compared to a previously reported native CD-CSP based on a triazole linkage, the current cationic CD-CSP shows a stronger retention and higher resolution towards acidic chiral compounds, ascribed to the propitious strong electrostatic attraction. Stability evaluation results indicated that thiol-ene reaction can provide a facile and robust approach for the preparation of positively charged CD CSPs.
authors.affiliation: Department of Chemistry, School of Science, Tianjin University, Tianjin 300072, China.
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Yao
authors.affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637459, Singapore.
authors.forename: Timothy Thatt Yang
authors.initials: TT
authors.lastname: Tan
authors.affiliation: Department of Chemistry, School of Science, Tianjin University, Tianjin 300072, China; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, China. Electronic address: wangyongtju@tju.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002264:Carboxylic Acids / Q000032:analysis; D002412:Cations; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D057930:Click Chemistry; D003505:Cyclodextrins / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000032:analysis; D009994:Osmolar Concentration; D012822:Silicon Dioxide / Q000737:chemistry*; D055672:Static Electricity; D013237:Stereoisomerism; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013844:Thiazoles / Q000032:analysis
chemical_list: D000596:Amino Acids; D002264:Carboxylic Acids; D002412:Cations; D003505:Cyclodextrins; D005419:Flavonoids; D007093:Imidazoles; D013438:Sulfhydryl Compounds; D013844:Thiazoles; D012822:Silicon Dioxide
keywords: Cationic cyclodextrin; Chiral stationary phase; HPLC; Thiol–ene click chemistry