title: Red sludge-induced mRNA and miRNA expression alterations in vital organs of CBA/Ca mice.
abstract: Several studies have investigated the ecological and genotoxic effects of the red mud accident in 2010 in Ajka, Hungary, but none was designed to reveal the early biological effects of red mud exposure at the level of early-responding gene expression. To address relevant questions, in the present study expression alterations of oncogenes (c-myc, K-ras), tumor suppressor genes (Bcl2, p53) and apoptosis-regulatory micro(mi) RNAs (miR-21, miR-27a, miR-93, miR-221) were analized 1, 3, 6 and 24 h after a single intraperitoneal injection of red mud to CBA/Ca mice. We observed changes in the expression of all investigated mRNAs, miR-21 and miR-221 in the liver, spleen, lung, kidney and lymph nodes of mice. An overexpression of the investigated genes was observed, but the level and the peak of the alteration differed according to examined tissue.
authors.affiliation: Institute of Public Health, Faculty of Medicine, University of Pécs, Pécs, 12 Szigeti str., 7624 Hungary. krisztina.juhasz01@gmail.com.
authors.forename: Antal
authors.initials: A
authors.lastname: Tibold
authors.forename: Krisztina
authors.initials: K
authors.lastname: Juhász
authors.forename: Katalin
authors.initials: K
authors.lastname: Gombos
authors.forename: Katalin
authors.initials: K
authors.lastname: Gocze
authors.forename: István
authors.initials: I
authors.lastname: Ember
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects*; D016147:Genes, Tumor Suppressor / Q000187:drug effects; D007274:Injections, Intraperitoneal; D051379:Mice; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009857:Oncogenes / Q000187:drug effects; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D012722:Sewage; D014018:Tissue Distribution
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger; D012722:Sewage
keywords: Red mud; early biological effect; gene expression; microRNA