title: Low-coverage MiSeq next generation sequencing reveals the mitochondrial genome of the Eastern Rock Lobster, Sagmariasus verreauxi.
abstract: The complete mitochondrial genome of the Eastern Rock lobster, Sagmariasus verreauxi, is reported for the first time. Using low-coverage, long read MiSeq next generation sequencing, we constructed and determined the mtDNA genome organization of the 15,470 bp sequence from two isolates from Eastern Tasmania, Australia and Northern New Zealand, and identified 46 polymorphic nucleotides between the two sequences. This genome sequence and its genetic polymorphisms will likely be useful in understanding the distribution and population connectivity of the Eastern Rock Lobster, and in the fisheries management of this commercially important species.
authors.affiliation: a Department of Genetics , La Trobe Institute for Molecular Science, La Trobe University , Bundoora , Victoria , Australia.
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Doyle
authors.affiliation: a Department of Genetics , La Trobe Institute for Molecular Science, La Trobe University , Bundoora , Victoria , Australia.
authors.forename: Ian S
authors.initials: IS
authors.lastname: Griffith
authors.affiliation: a Department of Genetics , La Trobe Institute for Molecular Science, La Trobe University , Bundoora , Victoria , Australia.
authors.forename: Nick P
authors.initials: NP
authors.lastname: Murphy
authors.affiliation: a Department of Genetics , La Trobe Institute for Molecular Science, La Trobe University , Bundoora , Victoria , Australia.
authors.forename: Jan M
authors.initials: JM
authors.lastname: Strugnell
mesh_terms: D000818:Animals; D001483:Base Sequence; D054629:Genome, Mitochondrial; D059014:High-Throughput Nucleotide Sequencing; D008969:Molecular Sequence Data; D033521:Palinuridae / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods; D012689:Sequence Homology, Nucleic Acid
keywords: Complete mitochondrial genome; Eastern Rock Lobster; MiSeq; Sagmariasus verreauxi; next generation sequencing