title: The interaction between tropomyosin-related kinase B receptors and serine kinases modulates acetylcholine release in adult neuromuscular junctions.
abstract: We conducted an electrophysiological study of the functional link between the tropomyosin-related kinase B (trkB) receptor signaling mechanism and serine-threonine kinases, both protein kinase C (PKC) and protein kinase A (PKA). We describe their coordinated role in transmitter release at the neuromuscular junction (NMJ) of the Levator auris longus muscle of the adult mouse. The trkB receptor normally seems to be coupled to stimulate ACh release because inhibiting the trkB receptor with K-252a results in a significant reduction in the size of EPPs. We found that the intracellular PKC pathway can operate as in basal conditions (to potentiate ACh release) without the involvement of the trkB receptor function, although the trkB pathway needs an operative PKC pathway if it is to couple to the release mechanism and potentiate it. To actively stimulate PKA (which also results in ACh release potentiation), the operativity of trkB is a necessary condition, and one effect of trkB may be PKA stimulation.
authors.affiliation: Unitat Histologia i Neurobiologia (UHN), Facultat de Medicina i Ciencies de la Salut, Universitat Rovira i Virgili, carrer St. Llorenç num 21, 43201 Reus, Spain. Electronic address: manuel.santafe@urv.cat.
authors.forename: Manel M
authors.initials: MM
authors.lastname: Santafé
authors.affiliation: Unitat Histologia i Neurobiologia (UHN), Facultat de Medicina i Ciencies de la Salut, Universitat Rovira i Virgili, carrer St. Llorenç num 21, 43201 Reus, Spain.
authors.forename: Neus
authors.initials: N
authors.lastname: Garcia
authors.affiliation: Unitat Histologia i Neurobiologia (UHN), Facultat de Medicina i Ciencies de la Salut, Universitat Rovira i Virgili, carrer St. Llorenç num 21, 43201 Reus, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Tomàs
authors.affiliation: Unitat Histologia i Neurobiologia (UHN), Facultat de Medicina i Ciencies de la Salut, Universitat Rovira i Virgili, carrer St. Llorenç num 21, 43201 Reus, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Obis
authors.affiliation: Unitat Histologia i Neurobiologia (UHN), Facultat de Medicina i Ciencies de la Salut, Universitat Rovira i Virgili, carrer St. Llorenç num 21, 43201 Reus, Spain.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Lanuza
authors.affiliation: Unitat Histologia i Neurobiologia (UHN), Facultat de Medicina i Ciencies de la Salut, Universitat Rovira i Virgili, carrer St. Llorenç num 21, 43201 Reus, Spain.
authors.forename: Nuria
authors.initials: N
authors.lastname: Besalduch
authors.affiliation: Unitat Histologia i Neurobiologia (UHN), Facultat de Medicina i Ciencies de la Salut, Universitat Rovira i Virgili, carrer St. Llorenç num 21, 43201 Reus, Spain. Electronic address: jmtf@fmcs.urv.es.
authors.forename: Josep
authors.initials: J
authors.lastname: Tomàs
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D019706:Excitatory Postsynaptic Potentials; D008297:Male; D051379:Mice; D009469:Neuromuscular Junction / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D020813:Receptor, trkB / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D020813:Receptor, trkB; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C; D000109:Acetylcholine
keywords: Brain-derived neurotrophic factor; Protein kinase A; Protein kinase C