title: P3N-PIPO of Clover yellow vein virus exacerbates symptoms in pea infected with white clover mosaic virus and is implicated in viral synergism.
abstract: Mixed infection of pea (Pisum sativum) with Clover yellow vein virus (ClYVV) and White clover mosaic virus (WClMV) led to more severe disease symptoms (a phenomenon called viral synergism). Similar to the mixed ClYVV/WClMV infection, a WClMV-based vector encoding P3N-PIPO of ClYVV exacerbated the disease symptoms. Infection with the WClMV vector encoding ClYVV HC-Pro (a suppressor of RNA silencing involved in potyviral synergisms), also resulted in more severe symptoms, although to a lesser extent than infection with the vector encoding P3N-PIPO. Viral genomic RNA accumulated soon after inoculation (at 2 and 4 days) at higher levels in leaves inoculated with WClMV encoding HC-Pro but at lower levels in leaves inoculated with WClMV encoding P3N-PIPO than in peas infected with WClMV encoding GFP. Our results suggest that ClYVV P3N-PIPO is involved in the synergism between ClYVV and WClMV during pea infection through an unknown mechanism different from suppression of RNA silencing.
authors.affiliation: Pathogen-Plant Interactions Group, Plant Breeding Science, Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Hisa
authors.affiliation: Pathogen-Plant Interactions Group, Plant Breeding Science, Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Haruka
authors.initials: H
authors.lastname: Suzuki
authors.affiliation: Iwate Biotechnology Research Center, Kitakami 024-0003, Iwate, Japan.
authors.forename: Go
authors.initials: G
authors.lastname: Atsumi
authors.affiliation: Pathogen-Plant Interactions Group, Plant Breeding Science, Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Choi
authors.affiliation: Pathogen-Plant Interactions Group, Plant Breeding Science, Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan. Electronic address: knakahar@res.agr.hokudai.ac.jp.
authors.forename: Kenji S
authors.initials: KS
authors.lastname: Nakahara
authors.affiliation: Pathogen-Plant Interactions Group, Plant Breeding Science, Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Uyeda
mesh_terms: D020816:Amino Acid Motifs; D060085:Coinfection / Q000821:virology*; D016366:Open Reading Frames; D018532:Pisum sativum / Q000821:virology*; D010935:Plant Diseases / Q000821:virology*; D017863:Potexvirus / Q000235:genetics / Q000502:physiology*; D017800:Potyvirus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D014764:Viral Proteins
keywords: HC-Pro; P3N-PIPO; Plant virus; Potexvirus; Potyvirus; Synergism; Virulence