title: Streptomyces barkulensis sp. nov., isolated from an estuarine lake.
abstract: The taxonomic position of a novel actinomycete, strain RC 1831(T), isolated from the sediment of a fish dumping yard at Barkul village near Chilika Lake, Odisha, India, was determined by a polyphasic approach. Based on morphological and chemotaxonomic characteristics the isolate was determined to belong to the genus Streptomyces. The phylogenetic tree based on its nearly complete 16S rRNA gene sequence (1428 nt) with representative strains showed that the strain consistently falls into a distinct phyletic line together with Streptomyces glaucosporus DSM 41689(T) (98.22% similarity) and a subclade consisting of Streptomyces atacamensis DSM 42065(T) (98.40%), Streptomyces radiopugnans R97 DSM 41901(T) (98.27%), Streptomyces fenghuangensis GIMN4.003(T) (98.33 %), Streptomyces nanhaiensis DSM 41926(T) (98.13%), Streptomyces megasporus NBRC 14749(T) (97.37%) and Streptomyces macrosporus NBRC 14748(T) (98.22%). However, the levels of DNA-DNA relatedness between strain RC 1831(T) and phylogenetically related strains Streptomyces atacamensis DSM 42065(T) (28.75 ± 3.25%) and Streptomyces glaucosporus DSM 41689(T) (15 ± 2.40%) were significantly lower than the 70% threshold value for delineation of genomic species. Furthermore, the isolate could be distinguished phenotypically on the basis of physiological, morphological and biochemical differences from its closest phylogenetic neighbours and other related reference strains. Strain RC 1831(T) is therefore considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces barkulensis sp. nov. is proposed. The type strain is RC 1831(T) ( = JCM 18754(T) = DSM 42082(T)).
authors.affiliation: School of Biotechnology, KIIT University, Bhubaneswar - 751024, Odisha, India.
authors.forename: Lopamudra
authors.initials: L
authors.lastname: Ray
authors.affiliation: School of Biotechnology, KIIT University, Bhubaneswar - 751024, Odisha, India.
authors.forename: Samir Ranjan
authors.initials: SR
authors.lastname: Mishra
authors.affiliation: School of Biotechnology, KIIT University, Bhubaneswar - 751024, Odisha, India.
authors.forename: Ananta Narayan
authors.initials: AN
authors.lastname: Panda
authors.affiliation: Chilika Development Authority, Plot No. C-11, BJB Nagar, Bhubaneswar - 751014, Odisha, India.
authors.forename: Gurdeep
authors.initials: G
authors.lastname: Rastogi
authors.affiliation: Chilika Development Authority, Plot No. C-11, BJB Nagar, Bhubaneswar - 751014, Odisha, India.
authors.forename: Ajit Kumar
authors.initials: AK
authors.lastname: Pattanaik
authors.affiliation: School of Biotechnology, KIIT University, Bhubaneswar - 751024, Odisha, India.
authors.forename: Tapan Kumar
authors.initials: TK
authors.lastname: Adhya
authors.affiliation: School of Biotechnology, KIIT University, Bhubaneswar - 751024, Odisha, India.
authors.forename: Mrutyunjay
authors.initials: M
authors.lastname: Suar
authors.affiliation: School of Biotechnology, KIIT University, Bhubaneswar - 751024, Odisha, India.
authors.forename: Vishakha
authors.initials: V
authors.lastname: Raina
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D063366:Estuaries; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology; D007194:India; D060106:Lakes / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9