title: Differential patterns of expression of neuropeptide Y throughout abstinence in outbred Swiss mice classified as susceptible or resistant to ethanol-induced locomotor sensitization.
abstract: Several studies have focused on the negative emotional state associated with drug abstinence. The peptide NPY plays an important role given its involvement in drug addiction, anxiety, and mood disorders. Interestingly, it is well established that outbred Swiss mice exhibit a prominent behavioral variability to ethanol-induced locomotor sensitization. Here, we investigated whether mice that were either susceptible or resistant to ethanol sensitization differed in their NPY expression during abstinence. The mice were treated daily with ethanol (2 g/kg, i.p.) or saline for 21 days. According to the locomotor activity after the last injection, the ethanol group was classified as sensitized (EtOH_High) or non-sensitized (EtOH_Low). To evaluate NPY expression, some of the mice were sacrificed at 18 h or 5 days of abstinence, and others were challenged at the 5th day of abstinence with ethanol (1.4 g/kg) and sacrificed after 1.5 h. At 5 days of abstinence, NPY expression increased in the orbital cortex, dorsomedial striatum, and dentate gyrus in the EtOH_High mice. These changes were counteracted by the ethanol challenge. In the EtOH_Low mice, NPY expression increased in the dentate gyrus only after 18 h of abstinence. Lastly, a decreased level of NPY was found in the prelimbic cortex of the EtOH_Low mice at 5 days of abstinence, and this was reversed by ethanol challenge. Therefore, behavioral variability in ethanol sensitization confers differential neurochemical features during the subsequent abstinence, including distinct patterns of NPY expression.
authors.affiliation: Department of Psychiatric and Medical Psychology, Federal University of São Paulo, Rua Borges Lagoa, 570, São Paulo, SP 04038-020, Brazil.
authors.forename: Ricardo Fontão
authors.initials: RF
authors.lastname: de Pauli
authors.affiliation: Laboratory of Neurobiology, Federal University of São Paulo, Rua Pedro de Toledo, 669, São Paulo, SP 04039-032, Brazil.
authors.forename: Cássia Canha
authors.initials: CC
authors.lastname: Coelhoso
authors.affiliation: Laboratory of Neurobiology, Federal University of São Paulo, Rua Pedro de Toledo, 669, São Paulo, SP 04039-032, Brazil.
authors.forename: Carolina
authors.initials: C
authors.lastname: Tesone-Coelho
authors.affiliation: Department of Physiological Science, Faculty of Medical Science of Santa Casa of São Paulo, Rua Cesário Mota Jr., 61, 12 andar, São Paulo, SP 01221-020, Brazil.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Linardi
authors.affiliation: Laboratory of Neurobiology, Federal University of São Paulo, Rua Pedro de Toledo, 669, São Paulo, SP 04039-032, Brazil.
authors.forename: Luiz Eugênio
authors.initials: LE
authors.lastname: Mello
authors.affiliation: Department of Psychiatric and Medical Psychology, Federal University of São Paulo, Rua Borges Lagoa, 570, São Paulo, SP 04038-020, Brazil.
authors.forename: Dartiu Xavier
authors.initials: DX
authors.lastname: Silveira
authors.affiliation: Laboratory of Neurobiology, Federal University of São Paulo, Rua Pedro de Toledo, 669, São Paulo, SP 04039-032, Brazil; Department of Physiological Science, Faculty of Medical Science of Santa Casa of São Paulo, Rua Cesário Mota Jr., 61, 12 andar, São Paulo, SP 01221-020, Brazil. Electronic address: guilherme.stos.jr@gmail.com.
authors.forename: Jair Guilherme
authors.initials: JG
authors.lastname: Santos
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D002540:Cerebral Cortex / Q000737:chemistry; D000431:Ethanol / Q000494:pharmacology*; D006624:Hippocampus / Q000737:chemistry; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000187:drug effects*; D009478:Neuropeptide Y / Q000032:analysis* / Q000502:physiology
chemical_list: D009478:Neuropeptide Y; D000431:Ethanol
keywords: Behavioral variability; Ethanol abstinence; Locomotor sensitization; Mice; Neuropeptide Y