title: [Mammalian target of rapamycin regulates androgen receptor and Akt phosphorylation in prostate cancer 22RV1 cells].
abstract: OBJECTIVE
  To investigate the roles of the mammalian target of rapamycin-1 and -2 (mTORC1 and TORC2) in the proliferation and apoptosis of prostate cancer 22RV1 cells.
  
  
  METHODS
  After silencing mTORC1 and TORC2, we examined the proliferation and apoptosis of prostate cancer 22RV1 cells by methylthiazol tetrazolium (MTT) assay and flow cytometry, respectively, and detected the expressions of the androgen receptor (AR) and Akt phosphorylation in the prostate cancer 22RV1 cells by Western blot after transfecting Raptor-siRNA and Rictor-siRNA to the 22RV1 cells.
  
  
  RESULTS
  MTT showed that the prostate cancer 22RV1 cells had no significant change in the growth rate after mTORC1 silence (P > 0.05), but their proliferation was markedly inhibited after mTORC2 silence (P < 0.01). Flow cytometry revealed a dramatic increase in the apoptosis of the 22RV1 cells after mTORC1 silence (P < 0.01), but no obvious change after mTORC2 silence (P > 0.05). Western blot exhibited that mTORC1 silence significantly increased the expression of AR and Akt phosphorylation (P < 0.05), while mTORC2 silence markedly decreased them (P < 0.05).
  
  
  CONCLUSIONS
  mTORC2 is not only required for the survival of prostate cancer 22RV1 cells, but also a promising therapeutic target of prostate cancer.
authors.affiliation: Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China.
authors.forename: Teng-Fei
authors.initials: TF
authors.lastname: Pan
authors.affiliation: Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China.
authors.forename: Chao-Zhao
authors.initials: CZ
authors.lastname: Liang
authors.affiliation: Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China.
authors.forename: Xian-Guo
authors.initials: XG
authors.lastname: Chen
authors.affiliation: Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China.
authors.forename: Song
authors.initials: S
authors.lastname: Fan
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D008297:Male; D000076222:Mechanistic Target of Rapamycin Complex 1; D000076225:Mechanistic Target of Rapamycin Complex 2; D046912:Multiprotein Complexes / Q000378:metabolism*; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D011944:Receptors, Androgen / Q000378:metabolism*; D020123:Sirolimus / Q000494:pharmacology*; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*
chemical_list: C410798:AR protein, human; D046912:Multiprotein Complexes; D011944:Receptors, Androgen; D000076222:Mechanistic Target of Rapamycin Complex 1; D000076225:Mechanistic Target of Rapamycin Complex 2; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases; D020123:Sirolimus