title: Role of EZH2 in oral squamous cell carcinoma carcinogenesis.
abstract: Oral squamous cell carcinoma (OSCC) is a common human malignancy with high incidence rate and poor prognosis. Although the polycomb group protein enhancer of zeste homolog 2 (EZH2) plays a crucial role in cell proliferation and differentiation during the occurrence and development progress of several kinds of malignant tumors, the impact of EZH2 on the development and progression of OSCC is unclear. In this study, we demonstrate that EZH2 is overexpressed in OSCC cells and clinical tissue. With in vitro RNAi analysis, we generated stable EZH2 knocking down cell lines from two OSCC cell lines, with two sh-RNAs targeting to EZH2, respectively. We found that knocking down of EZH2 could decrease the proliferation ability and induce apoptosis of OSCC cells. Moreover, we demonstrated that of EZH2 inhibition decreased the migration and metastasis of OSCC cells. In conclusion, the results of the current study demonstrated an association between EZH2 expression and OSCC cell development. We recommend that EZH2 acts as an oncogene and plays an important role in OSCC carcinogenesis.
authors.affiliation: Department of Dentistry, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China.
authors.forename: Lingbo
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Laboratory of Medical Genetics, Harbin Medical University, Harbin 150081, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Laboratory of Medical Genetics, Harbin Medical University, Harbin 150081, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
authors.affiliation: Laboratory of Medical Genetics, Harbin Medical University, Harbin 150081, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Bai
authors.affiliation: Laboratory of Medical Genetics, Harbin Medical University, Harbin 150081, China.
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Dentistry, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China.
authors.forename: Chunming
authors.initials: C
authors.lastname: Li
authors.affiliation: Laboratory of Medical Genetics, Harbin Medical University, Harbin 150081, China. Electronic address: sunwj@ems.hrbmu.edu.cn.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Sun
authors.affiliation: Department of Dentistry, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China. Electronic address: hrbwangmei@163.com.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D000071221:Enhancer of Zeste Homolog 2 Protein; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D009062:Mouth Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D009857:Oncogenes; D063151:Polycomb Repressive Complex 2 / Q000235:genetics* / Q000378:metabolism
chemical_list: C498409:EZH2 protein, human; D000071221:Enhancer of Zeste Homolog 2 Protein; D063151:Polycomb Repressive Complex 2
keywords: DMEM; Dulbecco's modified Eagle's medium; EGFR; EZH2; FBS; GAPDH; H3K27; HNSCC; OSCC; Oncogene; Oral squamous cell carcinoma; PVDF; a specific histone 3 lysine 27; enhancer of zeste homolog 2; epithelial growth factor receptor; fetal bovine serum; glyceraldehyde-3-phosphate dehydrogenase; head and neck squamous cell carcinoma; oral squamous cell carcinoma; polyvinylidene difluoride; qRT-PCR; quantitative reverse transcription polymerase chain reaction