title: Use of Induced sputum to determine the prevalence of Pneumocystis jirovecii in immunocompromised children with pneumonia.
abstract: BACKGROUND
  Information on prevalence of Pneumocystis jirovecii pneumonia (PCP) in immunocompromised children with pneumonia in Southeast Asia is limited.
  
  
  METHODS
  Immunocompromised children hospitalized with radiographic pneumonia were investigated for PCP by testing induced sputum by using polymerase chain reaction (PCR).
  
  
  RESULTS
  Ninety-four immunocompromised children (mean age 74.5 ± 43.7 months, boys 69) with pneumonia were investigated for PCP. Underlying disease included solid tumors and hematological malignancy in 57, HIV infection in 14, primary immune deficiency in 11 and other immune deficiency disorders in 12 children. PCR could detect P. jirovecii in 14 children. Prevalence of PCP in HIV-infected children was 43% (6 of 14), renal disease on immunosuppressants 45% (4 of 9), primary immune deficiency 19% (2 of 11) and malignancies on chemotherapy 4% (2 of 57). Three of 14 children died from PCP.
  
  
  CONCLUSIONS
  PCP is responsible for pneumonia in 14% of children with underlying immunocompromised state; PCR on induced sputum improves diagnosis.
authors.affiliation: Department of Pediatrics, All India Institute of Medical Sciences, New Delhi 110029, India.
authors.forename: Chandan K
authors.initials: CK
authors.lastname: Das
authors.affiliation: Department of Microbiology, All India Institute of Medical Sciences, New Delhi 110029, India.
authors.forename: Bijay R
authors.initials: BR
authors.lastname: Mirdha
authors.affiliation: Department of Microbiology, All India Institute of Medical Sciences, New Delhi 110029, India.
authors.forename: Sundeep
authors.initials: S
authors.lastname: Singh
authors.affiliation: Department of Microbiology, All India Institute of Medical Sciences, New Delhi 110029, India.
authors.forename: Rachna
authors.initials: R
authors.lastname: Seth
authors.affiliation: Department of Pediatrics, All India Institute of Medical Sciences, New Delhi 110029, India.
authors.forename: Arvind
authors.initials: A
authors.lastname: Bagga
authors.affiliation: Department of Pediatrics, All India Institute of Medical Sciences, New Delhi 110029, India.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Lodha
authors.affiliation: Department of Pediatrics, All India Institute of Medical Sciences, New Delhi 110029, India skkabra@hotmail.com.
authors.forename: Sushil K
authors.initials: SK
authors.lastname: Kabra
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D015897:Comorbidity; D003430:Cross-Sectional Studies; D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics; D005260:Female; D015658:HIV Infections / Q000453:epidemiology; D006801:Humans; D016867:Immunocompromised Host; D007194:India / Q000453:epidemiology; D007223:Infant; D008297:Male; D008875:Middle Aged; D009894:Opportunistic Infections / Q000453:epidemiology*; D045363:Pneumocystis carinii / Q000235:genetics / Q000302:isolation & purification*; D011020:Pneumonia, Pneumocystis / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000379:methods*; D015995:Prevalence; D013183:Sputum / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal