title: Novel systems for tailored neurotrophic factor release based on hydrogel and resorbable glass hollow fibers.
abstract: A novel system for the release of neurotrophic factor into a nerve guidance channel (NGC) based on resorbable phosphate glass hollow fibers (50P2O5-30CaO-9Na2O-3SiO2-3MgO-2.5K2O-2.5TiO2 mol%) in combination with a genipin-crosslinked agar/gelatin hydrogel (A/G_GP) is proposed. No negative effect on the growth of neonatal olfactory bulb ensheathing cell line (NOBEC) as well as on the expression of pro- and anti-apoptotic proteins was measured in vitro in the presence of fiber dissolution products in the culture medium. For the release studies, fluorescein isothiocyanate-dextran (FD-20), taken as growth factor model molecule, was solubilized in different media and introduced into the fiber lumen exploiting the capillary action. The fibers were filled with i) FD-20/phosphate buffered saline (PBS) solution, ii) FD-20/hydrogel solution before gelation and iii) hydrogel before gelation, subsequently lyophilized and then filled with the FD-20/PBS solution. The different strategies used for the loading of the FD-20 into the fibers resulted in different release kinetics. A slower release was observed with the use of A/G_GP hydrogel. At last, poly(ε-caprolactone) (PCL) nerve guides containing the hollow fibers and the hydrogel have been fabricated.
authors.affiliation: Department of Applied Science and Technology, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, Italy; Department of Clinical and Biological Sciences and Cavalieri Ottolenghi Neuroscience Institute, University of Turin, Torino, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Novajra
authors.affiliation: Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Tonda-Turo
authors.affiliation: Department of Applied Science and Technology, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, Italy. Electronic address: chiara.vitale@polito.it.
authors.forename: C
authors.initials: C
authors.lastname: Vitale-Brovarone
authors.affiliation: Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Ciardelli
authors.affiliation: Department of Clinical and Biological Sciences and Cavalieri Ottolenghi Neuroscience Institute, University of Turin, Torino, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Geuna
authors.affiliation: Department of Clinical and Biological Sciences and Cavalieri Ottolenghi Neuroscience Institute, University of Turin, Torino, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Raimondo
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002452:Cell Count; D002460:Cell Line; D003911:Dextrans / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods*; D005240:Feasibility Studies; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000737:chemistry; D005898:Glass / Q000737:chemistry*; D048091:Guided Tissue Regeneration; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009830:Olfactory Bulb / Q000166:cytology; D011091:Polyesters / Q000737:chemistry; D051381:Rats; D012044:Regression Analysis
chemical_list: D003911:Dextrans; D009414:Nerve Growth Factors; D011091:Polyesters; C009753:fiberglass; C015219:fluorescein isothiocyanate dextran; C016240:polycaprolactone; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D016650:Fluorescein-5-isothiocyanate
keywords: Glass hollow fibers; Growth factor release; Hydrogel; In vitro test; Nerve guidance channel