title: MiR-34a regulates apoptosis in liver cells by targeting the KLF4 gene.
abstract: MicroRNAs (miRNAs) regulate gene expression by inhibiting translation or targeting messenger RNA (mRNA) for degradation in a posttranscriptional fashion. In this study, we show that ectopic expression of miR-34a-5p reduces the mRNA and protein levels of Krüppel-like factor 4 (KLF4). We also demonstrate that miR-34a targets the 3'-untranslated mRNA region of KLF4 and show that overexpression of miR-34a induces a significant level of apoptosis in BNL CL.2 cells exposed to doxorubicin or 10 Gy X-ray. Our data suggest that the effects of miR-34a on apoptosis occur due to the downregulation of KLF4.
authors.affiliation: Department of Radiation Medicine, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou City, China.
authors.forename: Qiu
authors.initials: Q
authors.lastname: Chen
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Tu
authors.forename: Lu Lin
authors.initials: LL
authors.lastname: Zheng
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Xue Yong
authors.initials: XY
authors.lastname: Zuo
authors.forename: Yong Ming
authors.initials: YM
authors.lastname: He
authors.forename: Shu Yu
authors.initials: SY
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.forename: Jian Ping
authors.initials: JP
authors.lastname: Cao
authors.forename: Feng Mei
authors.initials: FM
authors.lastname: Cui
authors.forename: Jun
authors.initials: J
authors.lastname: Hou
mesh_terms: D017209:Apoptosis / Q000235:genetics; D005786:Gene Expression Regulation; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; C522425:MIRN34 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger