title: Formononetin protects TBI rats against neurological lesions and the underlying mechanism.
abstract: Traumatic brain injury (TBI) is a major cause of disability or death worldwide, especially in the young. Thus, effective medication with few side effects needs to be developed. This work aimed to explore the potential benefits of formononetin (FN) on TBI rodent model and to discuss the regarding mechanism. These findings showed that FN effectively increased the activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in brain tissue of TBI rats (P<0.01), while it reduced intracephalic malonaldehyde (MDA), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) concentrations (P<0.01). Meanwhile, the hydrocephalus in the TBI rat was alleviated, and the injured nerve cell of the lesioned brain was reduced as showed in hematoxylin-eosin (HE) staining assay. In addition, the endogenous mRNA level of cyclooxygenase-2 (COX-2) in the brain of the TBI rat was significantly down-regulated (P<0.01). Furthermore, the protein expression of nuclear factor E2-related factor 2 (Nrf2) was effectively up-regulated (P<0.01). Taken together, we conclude that formononetin mediates the promising anti-TBI effects against neurocyte damage, which the underlying mechanisms are associated with inhibiting intracephalic inflammatory response and oxidative stress for neuroprotection.
authors.affiliation: Emergency Department, Western Hospital, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530007, PR China.
authors.forename: Zhengzhao
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Physiology and Neurobiology, Xinxiang Medical University, Xinxiang 453003, PR China.
authors.forename: Xianhong
authors.initials: X
authors.lastname: Dong
authors.affiliation: Emergency Department, Western Hospital, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530007, PR China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Emergency Department, Western Hospital, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530007, PR China.
authors.forename: Guang
authors.initials: G
authors.lastname: Zeng
authors.affiliation: Emergency Department, Western Hospital, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530007, PR China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Spine and Osteopathy Surgery Division, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Guangxi Matemal and Child Health Hospital, Nanning, Guangxi Zhuang Autonomous Region 530003, PR China.
authors.forename: Rubiao
authors.initials: R
authors.lastname: Qiu
authors.affiliation: Institute of Urology and Nephrology, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, PR China.
authors.forename: Linjian
authors.initials: L
authors.lastname: Mo
authors.affiliation: Emergency Department, Western Hospital, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530007, PR China. Electronic address: yeyu_doctor@126.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Ye
mesh_terms: D000818:Animals; D008297:Male; D051381:Rats; D001930:Brain Injuries / Q000150:complications; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D005978:Glutathione / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D007529:Isoflavones / Q000627:therapeutic use; D008315:Malondialdehyde / Q000378:metabolism; D009422:Nervous System Diseases / Q000209:etiology / Q000517:prevention & control; D018696:Neuroprotective Agents / Q000627:therapeutic use; D017208:Rats, Wistar; D012333:RNA, Messenger / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D051267:NF-E2-Related Factor 2 / Q000378:metabolism
chemical_list: D051546:Cyclooxygenase 2; C007768:formononetin; D005978:Glutathione; D015850:Interleukin-6; D007529:Isoflavones; D008315:Malondialdehyde; D018696:Neuroprotective Agents; C489691:Ptgs2 protein, rat; D012333:RNA, Messenger; D013482:Superoxide Dismutase; D014409:Tumor Necrosis Factor-alpha; D051267:NF-E2-Related Factor 2
keywords: Formononetin; Inflammation; Neuroprotection; Oxidative stress; Rat; Traumatic brain injury