title: A potential novel strategy, inhibition of vasopressin-induced VEGF secretion by relcovaptan, for decreasing the incidence of ovarian hyperstimulation syndrome in the hyperstimulated rat model.
abstract: OBJECTIVE
  To investigate the effects of V1A receptor antagonist through inhibition of vasopressin-induced VEGF secretion in an experimental model.
  
  
  METHODS
  Thirty rats were randomly divided into five groups. Four groups were given 10IU pregnant mare serum gonadotropin/day (sc) at 8:00-8:30am on days 22-25 of life. They were administered 30IU hCG at 8:00-8:30am on day 26 of life. On days 26 and 27 of life at 8:00am and 4:00pm, (ip) per animal, 50μg/kg/day GnRH antagonist in the GnRH antagonist group, 0.3mg relcovaptan in the high dose relcovaptan group, and 0.15mg relcovaptan in the low dose relcovaptan group were administered. The control group was given the same dosage of 0.9% saline solution (ip) on days 22-26 day of life. The main outcomes were weight gain, ovarian weights, peritoneal fluid VEGF values, corpus luteum count, and atretic follicle count.
  
  
  RESULTS
  Weight gain was highest in the OHSS group; it was almost twice as much in the OHSS group than it was in the control group. Ovarian weights were significantly lower in all treatment groups (p=0.03). There was no statistically significant difference in ovarian weights between the GnRH antagonist and relcovaptan groups (p=0.176). The evaluation of peritoneal fluid VEGF-A levels revealed statistically significant differences between levels in the treatment groups and in the OHSS group (p=0.005). Atretic follicle count in the OHSS group was significantly lower (p=0.048). In all treatment groups, CL counts were prominently lower than they were in the OHSS group (p=0.002).
  
  
  CONCLUSIONS
  Relcovaptan may be a novel strategy for decreasing risk of OHSS by inhibition of vasopressin-induced VEGF secretion through V1A receptor antagonist.
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Gazi University, Turkey. Electronic address: c_cenksoy@hotmail.com.
authors.forename: Cahit
authors.initials: C
authors.lastname: Cenksoy
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Gazi University, Turkey.
authors.forename: Pinar Ozcan
authors.initials: PO
authors.lastname: Cenksoy
authors.affiliation: Department of Pathology, Faculty of Medicine, Gazi University, Turkey.
authors.forename: Ozlem
authors.initials: O
authors.lastname: Erdem
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Gazi University, Turkey.
authors.forename: Banu
authors.initials: B
authors.lastname: Sancak
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Gazi University, Turkey.
authors.forename: Rifat
authors.initials: R
authors.lastname: Gursoy
mesh_terms: D000818:Animals; D065092:Antidiuretic Hormone Receptor Antagonists; D001202:Ascitic Fluid / Q000737:chemistry; D006063:Chorionic Gonadotropin / Q000008:administration & dosage; D003338:Corpus Luteum; D004195:Disease Models, Animal; D005260:Female; D005496:Follicular Atresia; D007987:Gonadotropin-Releasing Hormone / Q000037:antagonists & inhibitors; D006064:Gonadotropins, Equine / Q000008:administration & dosage; D007211:Indoles / Q000008:administration & dosage*; D009929:Organ Size; D016471:Ovarian Hyperstimulation Syndrome / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D010053:Ovary / Q000473:pathology; D010062:Ovulation Induction / Q000009:adverse effects / Q000379:methods; D011759:Pyrrolidines / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis / Q000378:metabolism*; D014667:Vasopressins / Q000502:physiology*; D015430:Weight Gain
chemical_list: D065092:Antidiuretic Hormone Receptor Antagonists; D006063:Chorionic Gonadotropin; D006064:Gonadotropins, Equine; D007211:Indoles; D011759:Pyrrolidines; D042461:Vascular Endothelial Growth Factor A; D014667:Vasopressins; D007987:Gonadotropin-Releasing Hormone; C082134:relcovaptan
keywords: OHSS; Prevention; Relcovaptan; V1a receptor antagonist; VEGF