title: Generation of a novel dendritic-cell vaccine using melanoma and squamous cancer stem cells.
abstract: We identified cancer stem cell (CSC)-enriched populations from murine melanoma D5 syngeneic to C57BL/6 mice and the squamous cancer SCC7 syngeneic to C3H mice using ALDEFLUOR/ALDH as a marker, and tested their immunogenicity using the cell lysate as a source of antigens to pulse dendritic cells (DCs). DCs pulsed with ALDH(high) CSC lysates induced significantly higher protective antitumor immunity than DCs pulsed with the lysates of unsorted whole tumor cell lysates in both models and in a lung metastasis setting and a s.c. tumor growth setting, respectively. This phenomenon was due to CSC vaccine-induced humoral as well as cellular anti-CSC responses. In particular, splenocytes isolated from the host subjected to CSC-DC vaccine produced significantly higher amount of IFNγ and GM-CSF than splenocytes isolated from the host subjected to unsorted tumor cell lysate pulsed-DC vaccine. These results support the efforts to develop an autologous CSC-based therapeutic vaccine for clinical use in an adjuvant setting.
authors.affiliation: Department of Surgery, University of Michigan.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Lu
authors.forename: Huimin
authors.initials: H
authors.lastname: Tao
authors.forename: Carolyn
authors.initials: C
authors.lastname: Xue
authors.forename: Seagal
authors.initials: S
authors.lastname: Teitz-Tennenbaum
authors.forename: John H
authors.initials: JH
authors.lastname: Owen
authors.forename: Jeffrey S
authors.initials: JS
authors.lastname: Moyer
authors.forename: Mark E P
authors.initials: ME
authors.lastname: Prince
authors.forename: Alfred E
authors.initials: AE
authors.lastname: Chang
authors.forename: Max S
authors.initials: MS
authors.lastname: Wicha
mesh_terms: D000818:Animals; D019496:Cancer Vaccines / Q000276:immunology* / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000276:immunology* / Q000473:pathology / Q000556:secondary / Q000628:therapy; D003713:Dendritic Cells / Q000276:immunology*; D008175:Lung Neoplasms / Q000276:immunology / Q000556:secondary / Q000628:therapy; D008546:Melanoma, Experimental / Q000276:immunology* / Q000473:pathology; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D014411:Neoplastic Stem Cells / Q000276:immunology*
chemical_list: D019496:Cancer Vaccines