title: Interleukin-17F attenuates H2O2-induced cell cycle arrest.
abstract: Interleukin IL-17F was expressed in colon epithelial cells and showed multiple functions in colon tumorigenesis. However, the role of IL-17F in colon cancer cell cycle progression remains unclear. In this study, we analyzed the effects of IL-17F on oxidant-induced cell cycle shift in human colon cancer cells. IL-17F overexpressing and wildtype HCT116 cells were challenged with H(2)O(2). Cell cycle distribution analysis showed IL-17F attenuated H(2)O(2)-induced G2/M phase arrest by inhibiting S to G2/M transition. We further checked expression levels of two critical cell cycle regulators p21 and p27. The results showed that IL-17F could inhibit H(2)O(2) induced p27 up-regulation. Meanwhile, IL-17F could increase the phosphorylation of p38 after H(2)O(2) treatment. The regulations of p27 level and p38 activity may contribute to the impaired G2/M phase arrest by IL-17F. Taken together, our findings extend IL-17F as an important factor in colon cancer development and provide new insight into the signaling pathway.
authors.affiliation: School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China. Electronic address: ztong@whu.edu.cn.
authors.forename: Zan
authors.initials: Z
authors.lastname: Tong
authors.affiliation: Zhongshan Hospital of Hubei Province, Wuhan 430033, China.
authors.forename: Huichao
authors.initials: H
authors.lastname: Yan
authors.affiliation: School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China.
authors.forename: Weihuang
authors.initials: W
authors.lastname: Liu
mesh_terms: D063646:Carcinogenesis; D059447:Cell Cycle Checkpoints; D003106:Colon / Q000276:immunology*; D015179:Colorectal Neoplasms / Q000188:drug therapy / Q000276:immunology* / Q000473:pathology; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D004847:Epithelial Cells / Q000276:immunology*; D005786:Gene Expression Regulation; D045325:HCT116 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D020381:Interleukin-17 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D010766:Phosphorylation; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D019076:Transgenes / Q000235:genetics; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D050759:Cyclin-Dependent Kinase Inhibitor p21; D020381:Interleukin-17; D018809:Proliferating Cell Nuclear Antigen; C413374:p27 antigen; D006861:Hydrogen Peroxide; D048051:p38 Mitogen-Activated Protein Kinases
keywords: Cell cycle; H(2)O(2); Interleukin-17F; p27; p38