title: Light-regulated phosphorylation of maize phosphoenolpyruvate carboxykinase plays a vital role in its activity.
abstract: Phosphoenolpyruvate carboxykinase (PEPCK)-the major decarboxylase in PEPCK-type C4 plants-is also present in appreciable amounts in the bundle sheath cells of NADP-malic enzyme-type C4 plants, such as maize (Zea mays), where it plays an apparent crucial role during photosynthesis (Wingler et al., in Plant Physiol 120(2):539-546, 1999; Furumoto et al., in Plant Mol Biol 41(3):301-311, 1999). Herein, we describe the use of mass spectrometry to demonstrate phosphorylation of maize PEPCK residues Ser55, Thr58, Thr59, and Thr120. Western blotting indicated that the extent of Ser55 phosphorylation dramatically increases in the leaves of maize seedlings when the seedlings are transferred from darkness to light, and decreases in the leaves of seedlings transferred from light to darkness. The effect of light on phosphorylation of this residue is opposite that of the effect of light on PEPCK activity, with the decarboxylase activity of PEPCK being less in illuminated leaves than in leaves left in the dark. This inverse relationship between PEPCK activity and the extent of phosphorylation suggests that the suppressive effect of light on PEPCK decarboxylation activity might be mediated by reversible phosphorylation of Ser55.
authors.affiliation: Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, No. 20 Nanxincun, Xiangshan, Beijing, 100093, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Chao
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Liu
authors.forename: Ying-Chang
authors.initials: YC
authors.lastname: Mei
authors.forename: Zhi-Fang
authors.initials: ZF
authors.lastname: Gao
authors.forename: Yi-Bo
authors.initials: YB
authors.lastname: Chen
authors.forename: Chun-Rong
authors.initials: CR
authors.lastname: Qian
authors.forename: Yu-Bo
authors.initials: YB
authors.lastname: Hao
authors.forename: Bai-Chen
authors.initials: BC
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D008027:Light; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D019764:Phosphoenolpyruvate Carboxykinase (ATP) / Q000737:chemistry / Q000378:metabolism*; D010766:Phosphorylation; D018515:Plant Leaves / Q000201:enzymology; D017386:Sequence Homology, Amino Acid; D003313:Zea mays / Q000201:enzymology*
chemical_list: D019764:Phosphoenolpyruvate Carboxykinase (ATP)