title: Cloning and Expression of Genes Encoding F107-C and K88-1NT Fimbrial Proteins of Enterotoxigenic Escherichia coli from Piglets.
abstract: We cloned two genes coding F107-C and K88-1NT fimbrial subunits from strains E. coli C and 1NT isolated from Thua Thien Hue province, Vietnam. The mature peptide of faeG gene from strain E. coli 1NT (called faeG-1NT) is 100 % similarity with faeG gene, while the CDS of fedA gene from strain C (called fedA-C) has a similarity of 97 % with the fedA gene. Expression of the faeG-1NT and fedA-C genes in E. coli BL21 Star™ (DE3) produced proteins of ~31 and 22 kDa, respectively. The effect of IPTG concentration on the K88-1NT and F107-C fimbriae production was investigated. The results showed that 0.5 mM IPTG is suitable for higher expression of K88-1NT subunit, while 0.75 mM IPTG strongly stimulated expression of F107-C subunit. The optimal induction time for expression was also examined. Generally, highest expression of K88-1NT subunit occurred after 6 h of induction, while that of F107-C subunit is after 14 h.
authors.affiliation: Institute of Resources, Environment and Biotechnology, Hue University, Hue, 47000 Vietnam.
authors.forename: Nguyen Hoang
authors.initials: NH
authors.lastname: Loc
authors.affiliation: Institute of Resources, Environment and Biotechnology, Hue University, Hue, 47000 Vietnam.
authors.forename: Le My Tieu
authors.initials: le MT
authors.lastname: Ngoc
authors.affiliation: Institute of Resources, Environment and Biotechnology, Hue University, Hue, 47000 Vietnam.
authors.forename: Tran Thuy
authors.initials: TT
authors.lastname: Lan
authors.affiliation: Institute of Resources, Environment and Biotechnology, Hue University, Hue, 47000 Vietnam.
authors.forename: Le Quoc
authors.initials: le Q
authors.lastname: Viet
authors.affiliation: Institute of Resources, Environment and Biotechnology, Hue University, Hue, 47000 Vietnam.
authors.forename: Le Duc
authors.initials: le D
authors.lastname: Thao
authors.affiliation: Institute of Resources, Environment and Biotechnology, Hue University, Hue, 47000 Vietnam.
authors.forename: Hoang Tan
authors.initials: HT
authors.lastname: Quang
authors.affiliation: Institute of Resources, Environment and Biotechnology, Hue University, Hue, 47000 Vietnam.
authors.forename: Dinh Thi Bich
authors.initials: DT
authors.lastname: Lan
authors.affiliation: College of Agriculture and Forestry, Hue University, Hue, 47000 Vietnam.
authors.forename: Phung Thang
authors.initials: PT
authors.lastname: Long
keywords: E. coli; F107-C; Fimbriae; K88-1NT; faeG; fedA