title: Stability of diluted adenosine solutions in polyolefin infusion bags.
abstract: BACKGROUND
  Intravenous or intracoronary adenosine is used in the cardiac catherization lab to achieve maximal coronary blood flow and determine fractional flow reserve.
  
  
  OBJECTIVE
  To determine the stability of adenosine 10 and 50 µg/mL in either 0.9% sodium chloride injection or 5% dextrose injection in polyolefin infusion bags stored at 2 temperatures, refrigeration (2°C-8°C) or controlled room temperature (20°C-25°C).
  
  
  METHODS
  Adenosine 10 µg/mL and 50 µg/mL solutions were prepared in 50 mL polyolefin infusion bags containing 0.9% sodium chloride injection or 5% dextrose injection and stored at controlled room temperature or under refrigeration. Each combination of concentration, diluent, and storage was prepared in triplicate. Samples were assayed using stability-indicating, reversed-phase high-performance liquid chromatography immediately at time 0 and at 24 hours, 48 hours, 7 days, and 14 days. Stability was defined as retaining 90% to 110% of the initial adenosine concentration. The samples were also visually inspected against a light background for clarity, color, and the presence of particulate matter.
  
  
  RESULTS
  After 14 days, all samples retained 99% to 101% of the initial adenosine concentration. No considerable change in pH or visual appearance was noted. The stability data indicated no significant loss of drug due to chemical degradation or physical interactions during storage.
  
  
  CONCLUSIONS
  Adenosine solutions of 10 and 50 µg/mL were stable for at least 14 days in 50 mL polyolefin infusion bags of 0.9% sodium chloride injection or 5% dextrose injection stored at controlled room temperature and refrigerated conditions.
authors.affiliation: Wegmans School of Pharmacy, St. John Fisher College, Rochester, New York.
authors.forename: Elise
authors.initials: E
authors.lastname: Almagambetova
authors.affiliation: Assistant Professor of Pharmacy Practice, Wegmans School of Pharmacy, St. John Fisher College, Rochester, New York.
authors.forename: David
authors.initials: D
authors.lastname: Hutchinson
authors.affiliation: The Ohio State University Wexner Medical Center, Columbus, Ohio.
authors.forename: Danielle M
authors.initials: DM
authors.lastname: Blais
authors.affiliation: Associate Professor of Pharmaceutical Sciences, Wegmans School of Pharmacy, St. John Fisher College, Rochester, New York.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhao
keywords: adenosine; concentration; fractional flow reserve; stability