title: New fluoranthene FLUN-550 as a fluorescent probe for selective staining and quantification of intracellular lipid droplets.
abstract: A new class of live cell permeant, nontoxic fluoranthene-based fluorescent probe (FLUN-550) having a high Stokes shift in aqueous medium has been discovered. It showed selective staining of lipid droplets (LDs, dynamic cytoplasmic organelles) at a low concentration without background noise in in vitro live cell imaging of 3T3-L1 preadipocytes, J774 macrophages, MCF7 breast cancer cells, and single-celled, parasitic protozoa Leishmania donovani promastigotes and in vivo nonparasitic soil nematode C. elegans.
authors.affiliation: Fluorescent Lab, Division of Medicinal and Process Chemistry, ‡Electron Microscopy Unit, §Division of Pharmaceutics, and ∥Division of Toxicology, CSIR-Central Drug Research Institute (CDRI) , Lucknow 226031, India.
authors.forename: Atul
authors.initials: A
authors.lastname: Goel
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Sharma
authors.forename: Manoj
authors.initials: M
authors.lastname: Kathuria
authors.forename: Arindam
authors.initials: A
authors.lastname: Bhattacharjee
authors.forename: Ashwni
authors.initials: A
authors.lastname: Verma
authors.forename: Prabhat R
authors.initials: PR
authors.lastname: Mishra
authors.forename: Aamir
authors.initials: A
authors.lastname: Nazir
authors.forename: Kalyan
authors.initials: K
authors.lastname: Mitra
mesh_terms: D000818:Animals; D000981:Antiprotozoal Agents / Q000737:chemistry* / Q000494:pharmacology; D017173:Caenorhabditis elegans / Q000737:chemistry* / Q000378:metabolism; D045744:Cell Line, Tumor; D005449:Fluorenes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008055:Lipids / Q000032:analysis / Q000737:chemistry*; D008264:Macrophages / Q000737:chemistry* / Q000378:metabolism / Q000469:parasitology; D015394:Molecular Structure; D011083:Polycyclic Compounds / Q000737:chemistry*; D013194:Staining and Labeling
chemical_list: D000981:Antiprotozoal Agents; D005449:Fluorenes; D005456:Fluorescent Dyes; D008055:Lipids; D011083:Polycyclic Compounds; C007738:fluoranthene