title: Efficacy of Platelet-Rich-Plasma (PRP) and Highly Purified Bovine Xenograft (Laddec(®)) Combination in Bone Regeneration after Cyst Enucleation: Radiological and Histological Evaluation.
abstract: OBJECTIVE
  The purpose of the present study was to evaluate the efficacy of adding platelet-rich plasma (PRP) to a new highly purified bovine allograft (Laddec(®)) in the bone regeneration of cystic bony defects augmented following cystectomy.
  
  
  METHODS
  Study sample included 20 patients undergoing cystectomy in which the bone defect was filled with PRP and Laddec(®). All patients were examined with periapical radiographs before operation and at follow-up. After 3 months, at re-entry surgery for implant placement, bone core was taken for histological and histomorphometric analysis.
  
  
  RESULTS
  The postoperative successive radiographs showed a good regeneration of bone in the height of bony defects with application of PRP to bone graft. By the first postoperative month, about 48% of the defect was filled, which gradually increased in each month and showed about 90% of defect-fill by 6 months. Histological and histomorphometric analysis, showed a significant presence of bone tissue and vessels, with newly formed bone in contact with anorganic bone particles. The mean volume of vital bone was 68 ± 1.6% and the mean percentage of vital bone was 48 ± 2.4%. The mean percentage of inorganic particles in tissues was 20 ± 1.2% of the total volume. All the samples analyzed did not evidence the presence of inflammatory cells.
  
  
  CONCLUSIONS
  The results of this study showed how the use of Laddec(®) in association with platelet-rich plasma allows bone regeneration and has a potential for routine clinical use for regeneration of cystic bony defects.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Catania University, Catania Italy.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Pappalardo
authors.affiliation: Freelance Researcher, S.C.S., Scientific Consulting Services, Rome Italy.
authors.forename: Renzo
authors.initials: R
authors.lastname: Guarnieri
keywords: cystectomy; histology; platelet-rich plasma; sintered bovine bone true bone ceramics; xenograft.