title: Method for quantifying alginate and determining release from a food vehicle in gastrointestinal digesta.
abstract: To assess the efficacy of alginate as a modifier of enzyme activity, a suitable method to quantify its release must be developed. This paper develops and assesses the ability of the Periodic Acid Schiffs (PAS) assay to quantify alginate, and its release from bread during digestion in a model gut. Control and alginate enriched (4% w/w wet dough) bread were used. A model gut replicating the mouth, stomach and small intestines was used. Standard curves were created for alginate in deionised H2O and model gut solutions using a modified PAS to remove interference. The PAS assay quantified alginate with excellent linearity (R(2)=0.99), and optical density range (0.02-0.5). There was a significant difference in alginate release at 180 min compared to 0 and 60 min. The data indicate the modified PAS assay is a simple method for quantifying alginate release and release rate from alginate enriched products.
authors.affiliation: Institute for Cell and Molecular Bioscience, Medical School, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle Upon Tyne NE2 4HH, United Kingdom. Electronic address: d.houghton.@ncl.ac.uk.
authors.forename: David
authors.initials: D
authors.lastname: Houghton
authors.affiliation: Institute for Cell and Molecular Bioscience, Medical School, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle Upon Tyne NE2 4HH, United Kingdom.
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Wilcox
authors.affiliation: Institute for Cell and Molecular Bioscience, Medical School, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle Upon Tyne NE2 4HH, United Kingdom.
authors.forename: Iain A
authors.initials: IA
authors.lastname: Brownlee
authors.affiliation: Institute for Cell and Molecular Bioscience, Medical School, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle Upon Tyne NE2 4HH, United Kingdom.
authors.forename: Peter
authors.initials: P
authors.lastname: Chater
authors.affiliation: Institute for Cell and Molecular Bioscience, Medical School, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle Upon Tyne NE2 4HH, United Kingdom.
authors.forename: Chris J
authors.initials: CJ
authors.lastname: Seal
authors.affiliation: Institute for Cell and Molecular Bioscience, Medical School, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle Upon Tyne NE2 4HH, United Kingdom.
authors.forename: Jeffrey P
authors.initials: JP
authors.lastname: Pearson
mesh_terms: D000464:Alginates / Q000737:chemistry*; D004063:Digestion; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D013270:Stomach / Q000503:physiopathology*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid
keywords: Alginate; Digestion; Model gut; Periodic acid Schiffs assay