title: IL-7 inhibits tumor growth by promoting T cell-mediated antitumor immunity in Meth A model.
abstract: Immune suppression is well documented during tumor progression, which includes loss of effect of T cells and expansion of T regulatory (Treg) cells. IL-7 plays a key role in the proliferation, survival and homeostasis of T cells and displays a potent antitumor activity in vivo. In the present study, we investigated the antitumor effect of IL-7 in Meth A model. IL-7 inhibited tumor growth and prolonged the survival of tumor-bearing mice with corresponding increases in the frequency of CD4 and CD8 T cells, Th1 (CD4(+)IFN-γ(+)), Tc1 (CD8(+)IFN-γ(+)) and T cells cytolytic activity against Meth A cells. Neutralization of CD4 or CD8 T cells reversed the antitumor benefit of IL-7. Furthermore, IL-7 decreased regulatory T Foxp3 as well as cells suppressive activity with a reciprocal increase in SMAD7. In addition, we observed an increase of the serum concentrations of IL-6 and IFN-γ, and a significant decrease of TGF-β and IL-10 after IL-7 treatment. Taken together, these results indicate that IL-7 augments T cell-mediated antitumor immunity and improves the effect of antitumor in Meth A model.
authors.affiliation: Department of Biochemistry, North Sichuan Medical University, China.
authors.forename: Jian-Cai
authors.initials: JC
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, China.
authors.forename: Guo-Bo
authors.initials: GB
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, China.
authors.forename: Shi-Min
authors.initials: SM
authors.lastname: Wang
authors.affiliation: Department of Thoracic Oncology, West China Hospital, West China Medical School, Sichuan University, China.
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Wan
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, China. Electronic address: weiyuquan1959@163.com.
authors.forename: Yu-Quan
authors.initials: YQ
authors.lastname: Wei
mesh_terms: D000818:Animals; D019138:Antibodies, Blocking / Q000008:administration & dosage; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D048708:Cell Growth Processes / Q000187:drug effects; D045744:Cell Line, Tumor; D016207:Cytokines / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004195:Disease Models, Animal; D005260:Female; D005354:Fibrosarcoma / Q000139:chemically induced / Q000276:immunology / Q000628:therapy*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D015851:Interleukin-7 / Q000008:administration & dosage*; D008213:Lymphocyte Activation / Q000187:drug effects; D008748:Methylcholanthrene / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D012878:Skin Neoplasms / Q000139:chemically induced / Q000276:immunology / Q000628:therapy*; D051906:Smad7 Protein / Q000235:genetics / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects* / Q000276:immunology
chemical_list: D019138:Antibodies, Blocking; D016207:Cytokines; D015851:Interleukin-7; D051906:Smad7 Protein; D008748:Methylcholanthrene
keywords: CD4 and CD8 T cells; IFN-γ; IL-7; Treg cell