title: Cultured 3T3L1 adipocytes dispose of excess medium glucose as lactate under abundant oxygen availability.
abstract: White adipose tissue (WAT) produces lactate in significant amount from circulating glucose, especially in obesity;Under normoxia, 3T3L1 cells secrete large quantities of lactate to the medium, again at the expense of glucose and proportionally to its levels. Most of the glucose was converted to lactate with only part of it being used to synthesize fat. Cultured adipocytes were largely anaerobic, but this was not a Warburg-like process. It is speculated that the massive production of lactate, is a process of defense of the adipocyte, used to dispose of excess glucose. This way, the adipocyte exports glucose carbon (and reduces the problem of excess substrate availability) to the liver, but the process may be also a mechanism of short-term control of hyperglycemia. The in vivo data obtained from adipose tissue of male rats agree with this interpretation.
authors.affiliation: 1] Department of Nutrition and Food Science, Faculty of Biology, University of Barcelona, Barcelona 08028, Spain [2] Institute of Biomedicine, University of Barcelona, Barcelona 08028, Spain [3] CIBER Obesity and Nutrition, Institute of Health Carlos III, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Sabater
authors.affiliation: 1] Department of Nutrition and Food Science, Faculty of Biology, University of Barcelona, Barcelona 08028, Spain [2] Institute of Biomedicine, University of Barcelona, Barcelona 08028, Spain [3] CIBER Obesity and Nutrition, Institute of Health Carlos III, Spain.
authors.forename: Sofía
authors.initials: S
authors.lastname: Arriarán
authors.affiliation: 1] Department of Nutrition and Food Science, Faculty of Biology, University of Barcelona, Barcelona 08028, Spain [2] Institute of Biomedicine, University of Barcelona, Barcelona 08028, Spain [3] CIBER Obesity and Nutrition, Institute of Health Carlos III, Spain.
authors.forename: María del Mar
authors.initials: Mdel M
authors.lastname: Romero
authors.affiliation: 1] Department of Nutrition and Food Science, Faculty of Biology, University of Barcelona, Barcelona 08028, Spain [2] Institute of Biomedicine, University of Barcelona, Barcelona 08028, Spain [3] CIBER Obesity and Nutrition, Institute of Health Carlos III, Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: Agnelli
authors.affiliation: 1] Department of Nutrition and Food Science, Faculty of Biology, University of Barcelona, Barcelona 08028, Spain [2] Institute of Biomedicine, University of Barcelona, Barcelona 08028, Spain [3] CIBER Obesity and Nutrition, Institute of Health Carlos III, Spain.
authors.forename: Xavier
authors.initials: X
authors.lastname: Remesar
authors.affiliation: 1] Department of Nutrition and Food Science, Faculty of Biology, University of Barcelona, Barcelona 08028, Spain [2] Institute of Biomedicine, University of Barcelona, Barcelona 08028, Spain [3] CIBER Obesity and Nutrition, Institute of Health Carlos III, Spain.
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Fernández-López
authors.affiliation: 1] Department of Nutrition and Food Science, Faculty of Biology, University of Barcelona, Barcelona 08028, Spain [2] Institute of Biomedicine, University of Barcelona, Barcelona 08028, Spain [3] CIBER Obesity and Nutrition, Institute of Health Carlos III, Spain.
authors.forename: Marià
authors.initials: M
authors.lastname: Alemany
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D002452:Cell Count; D002454:Cell Differentiation; D049109:Cell Proliferation; D003470:Culture Media; D004734:Energy Metabolism; D015870:Gene Expression; D005947:Glucose / Q000378:metabolism*; D045022:Lactate Dehydrogenases / Q000378:metabolism; D019344:Lactic Acid / Q000096:biosynthesis* / Q000097:blood; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D010101:Oxygen Consumption; D051381:Rats
chemical_list: D003470:Culture Media; D019344:Lactic Acid; D045022:Lactate Dehydrogenases; D005947:Glucose