title: Cloning and characterization of cold, salt and drought inducible C-repeat binding factor gene from a highly cold adapted ecotype of Lepidium latifolium L.
abstract: The dehydration-responsive element-binding (DREB) protein/C-Repeat Binding Factors (CBFs) belongs to APETALA2 (AP2) family transcription factors that binds to DRE/CRT cis-element in cold-responsive (COR) genes and induce COR genes. CBFs have been isolated and characterized from evolutionarily diverse plant species. CBF pathway is conserved by CBF regulon and the size or the number and kind of target genes vary among freezing sensitive and tolerant plants. Hence, cloning of CBFs from highly freezing tolerant plants such as Lepidium latifolium L. will be useful in understanding the freezing tolerance of this species. In this study, LlCBF, a CBF1 family gene from L. Latifolium L., was cloned using RT-PCR and RACE-PCR. The full length mRNA of LlCBF is 948 bp with an open reading frame of 642 bp, encoding a protein of 213 amino acids with a molecular weight of 23.92 kDa and a theoretical isoelectric point of 4.80. Amino acid sequence analysis showed that LlCBF has an AP2 DNA binding domain, a potential CBF type nuclear localization signal (NLS) and C-terminal acidic domain. Semi-quantitative RT-PCR analysis of LlCBF revealed that this gene is up-regulated by high salt, dehydration and low temperature stresses. The investigation is therefore successful in cloning of a gene having strong homology with CBF transcription factors and responsive to low temperature, high salt and dehydration conditions.
authors.affiliation: Department of Genetics and Plant Breeding, College of Agriculture, G. B. Pant University of Agriculture & Technology, Pantnagar, 263145 Udhamsingh Nagar, Uttarakhand India ; Department of Biotechnology, Faculty of Natural Sciences, Jamia Millia Islamia, New Delhi, India.
authors.forename: M
authors.initials: M
authors.lastname: Akhtar
authors.affiliation: Department of Genetics and Plant Breeding, College of Agriculture, G. B. Pant University of Agriculture & Technology, Pantnagar, 263145 Udhamsingh Nagar, Uttarakhand India.
authors.forename: A
authors.initials: A
authors.lastname: Jaiswal
authors.affiliation: Department of Genetics and Plant Breeding, College of Agriculture, G. B. Pant University of Agriculture & Technology, Pantnagar, 263145 Udhamsingh Nagar, Uttarakhand India.
authors.forename: J P
authors.initials: JP
authors.lastname: Jaiswal
authors.affiliation: Department of Biotechnology, Faculty of Natural Sciences, Jamia Millia Islamia, New Delhi, India.
authors.forename: M I
authors.initials: MI
authors.lastname: Qureshi
authors.affiliation: Department of Molecular Biology and Biotechnology, G. B. Pant University of Agriculture & Technology, Pantnagar, 263145 Udhamsingh Nagar, Uttarakhand India.
authors.forename: M
authors.initials: M
authors.lastname: Tufchi
authors.affiliation: Department of Genetics and Plant Breeding, College of Agriculture, G. B. Pant University of Agriculture & Technology, Pantnagar, 263145 Udhamsingh Nagar, Uttarakhand India.
authors.forename: N K
authors.initials: NK
authors.lastname: Singh
keywords: Cold; Lepidium latifolium L.; LlCBF transcription factor; Salt and drought inducible