title: Multiple action sites for photosystem II herbicides as revealed by delayed fluorescence.
abstract: DCMU (3-(3,4-dichlorophenyl)-1,1-dimethylurea) at concentrations higher than 10 μM suppresses the second time range delayed fluorescence (DF) of pea chloroplasts, due to inhibition of the oxidizing side of photosystem II (PS II). The inhibition of the reducing side of PS II resulting in the suppression of millisecond DF takes place at much lower (∼0.01 μM) DCMU concentrations. The variation in the herbicide-affinities of the reducing and oxidizing sides of PS II is not the same for DCMU and phenol-type herbicides. The DCMU-affinity of the oxidizing side considerably increases and approximates that of the reducing side upon mild treatment of chloroplasts with oleic acid. Probably this is a result of some changes in the environment of the binding site at the oxidizing side. At DCMU concentrations higher than 1 mM, the chaotropic action of DCMU leads to the generation of millisecond luminescence which is not related to the functioning of the reaction centres.
authors.affiliation: Department of Biophysics, Faculty of Biology, M.V. Lomonosov Moscow State University, 19899, Moscow, USSR.
authors.forename: I R
authors.initials: IR
authors.lastname: Vasil'ev
authors.forename: D N
authors.initials: DN
authors.lastname: Matorin
authors.forename: V V
authors.initials: VV
authors.lastname: Lyadsky
authors.forename: P S
authors.initials: PS
authors.lastname: Venediktov