title: Asymmetric bioreduction of keto esters by immobilized baker's yeast entrapped in calcium alginate beads in both water and organic/water solvent systems.
abstract: Immobilized baker's yeast entrapped in calcium alginate beads, ca 1.5 mm diameter, was used more than 10 times in water and reduced ethyl 3-oxobutanoate and ethyl benzoylformate to the corresponding chiral hydroxy esters in good chemical yields and in high enantioselectivities. The biocatalyst was also successfully used in organic/water solvent systems such as hexane/water and acetonitrile/water, and in other systems, particularly in hexane, converted keto esters into their individual chiral hydroxy esters.
authors.affiliation: Department of Biological Chemistry, Faculty of Science, Okayama University of Science, 1-1 Ridaicho, 700, Okayama, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Naoshima
authors.forename: Y
authors.initials: Y
authors.lastname: Munakata
authors.forename: T
authors.initials: T
authors.lastname: Nishiyama
authors.forename: J
authors.initials: J
authors.lastname: Maeda
authors.forename: M
authors.initials: M
authors.lastname: Kamezawa
authors.forename: T
authors.initials: T
authors.lastname: Haramaki
authors.forename: H
authors.initials: H
authors.lastname: Tachibana