title: AKR1B10, a good prognostic indicator in gastric cancer.
abstract: OBJECTIVE
  The aim of the study was to investigate the correlation between AKR1B10 expression and clinicopathological features of gastric cancer (GC).
  
  
  METHODS
  Real-time polymerase chain reaction (RT-PCR) was performed to determine AKR1B10 mRNA expression. AKR1B10 protein levels were measured by immunohistochemistry.
  
  
  RESULTS
  RT-PCR analysis confirmed that AKR1B10 was significantly down-regulated in gastric cancer compared with paired, normal mucosa. Immunohistochemistry revealed that the percentage of AKR1B10-positive specimens was lower in gastric carcinoma compared with normal specimens. The frequency of AKR1B10-positive GC specimens was higher in patients with tumor size <5 cm, no lymph node metastasis, no distant metastasis and lower tumor stages The mean survival time for patients in the AKR1B10-positive group was significantly higher compared with the AKR1B1-negative group. The 5-year survival rate for the AKR1B10-positive group was also significantly higher than for the AKR1B1-negative group. Cox regression analysis revealed that AKR1B10 expression is an independent prognostic factor of GC.
  
  
  CONCLUSIONS
  Expression of AKR1B10 in gastric cancer was significantly associated with tumor size, lymph node metastasis, distance metastasis and TNM stage, and AKR1B10 may be a good prognostic indicator in gastric cancer.
authors.affiliation: Department of Gastrointestinal Surgery, Zhejiang Provincial People's Hospital, Hangzhou 310014, Zhejiang, China.
authors.forename: H B
authors.initials: HB
authors.lastname: Yao
authors.affiliation: Department of Gastrointestinal Surgery, Zhejiang Provincial People's Hospital, Hangzhou 310014, Zhejiang, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Gastrointestinal Surgery, Zhejiang Provincial People's Hospital, Hangzhou 310014, Zhejiang, China.
authors.forename: L G
authors.initials: LG
authors.lastname: Chen
authors.affiliation: Department of Gastrointestinal Surgery, Zhejiang Provincial People's Hospital, Hangzhou 310014, Zhejiang, China.
authors.forename: T P
authors.initials: TP
authors.lastname: Guan
authors.affiliation: Key Laboratory of Gastroenterology of Zhejiang Province, Hangzhou 310014, Zhejiang, China.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Ma
authors.affiliation: Key Laboratory of Gastroenterology of Zhejiang Province, Hangzhou 310014, Zhejiang, China.
authors.forename: X J
authors.initials: XJ
authors.lastname: He
authors.affiliation: Key Laboratory of Gastroenterology of Zhejiang Province, Hangzhou 310014, Zhejiang, China.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Xia
authors.affiliation: Key Laboratory of Gastroenterology of Zhejiang Province, Hangzhou 310014, Zhejiang, China.
authors.forename: H-Q
authors.initials: HQ
authors.lastname: Tao
authors.affiliation: Department of Gastrointestinal Surgery, Zhejiang Provincial People's Hospital, Hangzhou 310014, Zhejiang, China. Electronic address: yhb0129@126.com.
authors.forename: Q S
authors.initials: QS
authors.lastname: Shao
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry* / Q000235:genetics* / Q000401:mortality / Q000556:secondary / Q000601:surgery; D000368:Aged; D000449:Aldehyde Reductase / Q000032:analysis* / Q000235:genetics; D000074408:Aldo-Keto Reductases; D014408:Biomarkers, Tumor / Q000032:analysis*; D001707:Biopsy, Needle; D016022:Case-Control Studies; D018572:Disease-Free Survival; D005260:Female; D005743:Gastrectomy / Q000379:methods; D005753:Gastric Mucosa / Q000473:pathology; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008198:Lymph Nodes / Q000737:chemistry / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000473:pathology; D009367:Neoplasm Staging; D011379:Prognosis; D016016:Proportional Hazards Models; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012016:Reference Values; D012189:Retrospective Studies; D018709:Statistics, Nonparametric; D013274:Stomach Neoplasms / Q000737:chemistry* / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D016019:Survival Analysis; D016610:Tissue Embedding; D059467:Transcriptome / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D005819:Genetic Markers; D012333:RNA, Messenger; C120471:AKR1B10 protein, human; D000074408:Aldo-Keto Reductases; D000449:Aldehyde Reductase
keywords: AKR1B10; Gastric cancer; Metastasis; Prognosis