title: [Influence of different preparation on the content of carbohydrate and physicochemical properties of polysaccharides from raw paeoniae radix alba and stir-baked paeoniae radix alba].
abstract: OBJECTIVE
  To observe the difference of two preparation methods on the content of carbohydrate and physicochemical properties of polysaccharides from raw Paeoniae Radix Alba and stir-baked Paeoniae Radix Alba.
  
  
  METHODS
  Polysaccharides extracted from raw Paeoniae Radix Alba and stir-baked Paeoniae Radix Alba with water were precipitated by ethanol and named as BSEP-S and BSEP-C, respectively. In the same way, those deposited by acetone were named as BSAP-S and BSAP-C. Their physicochemical properties, including extraction yield, the content of carbohydrate, elemental composition and monosaccharide composition, were determined.
  
  
  RESULTS
  Extraction yield, sugar content and uronic acid content of BSEP-S was 1.56%, 80.56% and 3.33% , BSEP-C 1.18%, 80.79% and 5.47%, BSAP-S 1.58%, 86.50% and 3.79%, and BSAP-C 1.54%, 81.64% and 3.25%, respectively. In addition, monosaccharide compositions showed that glucose was the main monosaccharide and successively reached 92.21%, 87.30%, 91.45% and 86.62%.
  
  
  CONCLUSIONS
  Yield and content of carbohydrate from raw Paeoniae Radix Alba and stir-baked Paeoniae Radix Alba by water extraction-ethanol precipitation are a little higher than that by water extraction-acetone precipitation, but monosaccharide compositions are almost the same. Different preparation has significant impact on the yield and the content of carbohydrate in Paeoniae Radix Alba by stir-baked method, and it can decrease the dissolution of polysaccharide.
authors.affiliation: Guangdong Institute for Food and Drug Control, Guangzhou 510180, China. wysnake30@126.com
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Guangzhou Medical University, Guangzhou 510182, China.
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Tao
authors.affiliation: Guangzhou Medical University, Guangzhou 510182, China.
authors.forename: Fa-Jiang
authors.initials: FJ
authors.lastname: Chen
authors.affiliation: Shanghai Institute of Organic Chemistry, Chinese Academy of Science, Shanghai 200032, China.
authors.forename: Geng-Yuan
authors.initials: GY
authors.lastname: Tian
mesh_terms: D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000737:chemistry; D029596:Paeonia / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D011134:Polysaccharides / Q000032:analysis* / Q000302:isolation & purification; D014574:Uronic Acids / Q000032:analysis / Q000302:isolation & purification; D014867:Water / Q000737:chemistry
chemical_list: D011134:Polysaccharides; D014574:Uronic Acids; D014867:Water; D000431:Ethanol