title: Short communication: Detection of non-culturable Vibrio cholerae O139, by PCR and fluorescent antibody methods, in laboratory microcosms.
abstract: Non-culturable Vibrio cholerae O139 was detected in microcosms by PCR and fluorescent-antibody (FA) techniques. When survival of V. cholerae O139 in microcosms was assessed by viable counting on culture media, the vibrio became non-culturable after 44 days and remained non-culturable for an additional 7 weeks.
authors.affiliation: Environmental Microbiology Laboratory, Laboratory Sciences Division, International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), GPO Box 128, 1000, Dhaka, Bangladesh.
authors.forename: M S
authors.initials: MS
authors.lastname: Islam
authors.forename: M J
authors.initials: MJ
authors.lastname: Alam
authors.forename: M A
authors.initials: MA
authors.lastname: Miah
authors.forename: A
authors.initials: A
authors.lastname: Felsenstein
authors.forename: R B
authors.initials: RB
authors.lastname: Sack