title: [Analysis and comparison of intestinal absorption of components of Gegenqinlian decoction in different combinations based on pharmacokinetic parameters].
abstract: To analyse and compare the characteristics of the intestinal absorption of puerarin, baicalin, berberine and liquiritin in different combinations of Gegenqinlian decoction based on pharmacokinetic parameters, a sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was applied for the quantification of four components in rat's plasma. And pharmacokinetic parameters were determined from the plasma concentration-time data with the DAS software package. The influence of different combinations on pharmacokinetics of four components was studied to analyse and compare the absorption difference of four components, together with the results of the in vitro everted gut model and the rat single pass intestinal perfusion model. The results showed that compared with other combinations, the AUC values of puerarin, baicalin and berberine were increased significantly in Gegenqinlian decoction group, while the AUC value of liquiritin was reduced. Moreover, the absorption of four components was increased significantly supported by the results from the in vitro everted gut model and the rat single pass intestinal perfusion model, which indicated that the Gegenqinlian decoction may promote the absorption of four components and accelerate the metabolism of liquiritin by the cytochrome P450.
authors.affiliation: Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Yi-Zhu
authors.initials: YZ
authors.lastname: Zhang
authors.affiliation: Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Rui
authors.initials: R
authors.lastname: An
authors.affiliation: Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Qing-Qing
authors.initials: QQ
authors.lastname: Gu
authors.affiliation: Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Xin-Hong
authors.initials: XH
authors.lastname: Wang
mesh_terms: D000818:Animals; D019540:Area Under Curve; D001599:Berberine / Q000097:blood / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D039323:Coptis / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000493:pharmacokinetics*; D044950:Flavanones / Q000097:blood / Q000493:pharmacokinetics; D005419:Flavonoids / Q000097:blood / Q000493:pharmacokinetics; D005960:Glucosides / Q000097:blood / Q000493:pharmacokinetics; D029882:Glycyrrhiza uralensis / Q000737:chemistry; D007408:Intestinal Absorption; D007529:Isoflavones / Q000097:blood / Q000493:pharmacokinetics; D008297:Male; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D029908:Pueraria / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D031364:Scutellaria baicalensis / Q000737:chemistry; D053719:Tandem Mass Spectrometry
chemical_list: D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids; D005960:Glucosides; D007529:Isoflavones; C542794:gegenqinlian; D001599:Berberine; C038044:baicalin; C512196:liquiritin; C033607:puerarin