title: [High-quality total RNA extraction from medicinal plant Aquilaria sinensis].
abstract: OBJECTIVE
  It is quite difficult to isolate total RNA from Aquilaria sinensis due to the complexity of its secondary metabolites. In order to obtain the high-quality RNA, we investigated sampling method and RNA extraction from both normal and agilawood tissues of the plant.
  
  
  METHODS
  Five methods such as modified CTAB-LiCl method, modified CTAB-LiCl method with no water-bath, SDS-acid phenol method, modified guanidinium thiocyanate-CTAB method and EASYspin RN09 RNA isolation kit were used to extract RNA from leaves, branches, white wood samples, agilawood samples of A. sinensis, respectively.
  
  
  RESULTS
  Total RNA was stable for 5 hours at room temperature after extracted from A. sinensis branches. EASYspin RN09 RNA isolation kit was the best extraction method for the leaf while modified guanidinium thiocyanate-CTAB method was the best for the other samples.
  
  
  CONCLUSIONS
  A great quantity of RNAs can be isolated from samples of white wood and agilawood wood, respectively. Their ratios of 28S : 18S are in the range of 1.2 - 1.4 and RINs are between 6.8 - 7.5. These RNAs can be applied in transcriptome sequencing.
authors.affiliation: State Key Laboratory of Applied Microbiology, South China (The Ministry-Province Joint Development), Guangdong Open Laboratory of Applied Microbiology/Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application/Guangdong Institute of Microbiology, Guangzhou 510070, China. whq0401@163.com
authors.forename: Hong-Qing
authors.initials: HQ
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Applied Microbiology, South China (The Ministry-Province Joint Development), Guangdong Open Laboratory of Applied Microbiology/Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application/Guangdong Institute of Microbiology, Guangzhou 510070, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Applied Microbiology, South China (The Ministry-Province Joint Development), Guangdong Open Laboratory of Applied Microbiology/Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application/Guangdong Institute of Microbiology, Guangzhou 510070, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.affiliation: College of Pharmacy,Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Gao
authors.affiliation: College of Science, Jiangxi Agricultural University, Nanchang 330045, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Bai
authors.affiliation: State Key Laboratory of Applied Microbiology, South China (The Ministry-Province Joint Development), Guangdong Open Laboratory of Applied Microbiology/Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application/Guangdong Institute of Microbiology, Guangzhou 510070, China.
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Zhang
mesh_terms: D006146:Guanidines; D018515:Plant Leaves / Q000737:chemistry / Q000235:genetics; D018547:Plant Stems / Q000737:chemistry / Q000235:genetics; D010946:Plants, Medicinal / Q000737:chemistry / Q000235:genetics*; D018749:RNA, Plant / Q000032:analysis / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet; D013861:Thiocyanates; D029645:Thymelaeaceae / Q000737:chemistry / Q000235:genetics*
chemical_list: D006146:Guanidines; D018749:RNA, Plant; D013861:Thiocyanates; C054436:guanidine thiocyanate