title: Coordination ligand exchange of a xanthene probe-Ce(III) complex for selective fluorescence sensing of inorganic pyrophosphate.
abstract: A fluorescence sensing system for inorganic pyrophosphate based on ligand exchange of the Ce(III) complex of a xanthene-type probe is developed. This sensing system is successfully applied to the fluorescence detection of polymerase-catalyzed DNA amplification using loop-mediated isothermal amplification.
authors.affiliation: Department of Biotechnology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand. Jirarut.chu@mahidol.ac.th.
authors.forename: Ekkachai
authors.initials: E
authors.lastname: Kittiloespaisan
authors.forename: Ippei
authors.initials: I
authors.lastname: Takashima
authors.forename: Wansika
authors.initials: W
authors.lastname: Kiatpathomchai
authors.forename: Jirarut
authors.initials: J
authors.lastname: Wongkongkatep
authors.forename: Akio
authors.initials: A
authors.lastname: Ojida
mesh_terms: D002563:Cerium / Q000737:chemistry*; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D011756:Diphosphates / Q000032:analysis*; D008024:Ligands; D015335:Molecular Probes / Q000737:chemistry*; D021141:Nucleic Acid Amplification Techniques; D013050:Spectrometry, Fluorescence; D014966:Xanthenes / Q000737:chemistry
chemical_list: D056831:Coordination Complexes; D011756:Diphosphates; D008024:Ligands; D015335:Molecular Probes; D014966:Xanthenes; D002563:Cerium; C107241:diphosphoric acid; D004247:DNA; D004259:DNA-Directed DNA Polymerase