title: Parenteral administration of twin-bearing ewes with L-arginine enhances the birth weight and brown fat stores in sheep.
abstract: The objective of this study was to evaluate the effects of parenteral administration of L-arginine (Arg) to well-fed twin-bearing ewes from day (d) 100 of pregnancy to birth on fetal growth, body composition and neonatal behavior. Ewes received an i.v. bolus of either 345 μmol Arg-HCl/kg bodyweight or saline solution (control) 3 times a day. At d 140 of pregnancy, Arg-supplemented and control ewes were euthanized and fetal weight and fetal organ weight recorded, and maternal and fetal plasma concentrations of amino acids, hormones and metabolites analyzed. A subset of ewes was allowed to lamb and birth weight, body dimensions and behavior of the lambs in the first 2 hours(h) following birth recorded and blood samples collected. At d 140 of pregnancy, fetal weight internal organ weights were unaffected by treatment with the exception of brown fat stores which were increased by 16% in fetuses from Arg-supplemented ewes relative to controls (P < 0.05). At birth, there was an interaction (P = 0.06) between treatment and sex for birth weight of the lamb. The ewe lambs from Arg-supplemented ewes were 12% (P < 0.05) heavier at birth compared with controls whereas birth weight of male lambs did not differ. These results indicate that maternal Arg supplementation enhanced brown fat stores in the fetus and countered some effect of fetal growth restriction due to litter size in female lambs. Increasing birth weight of female lambs and enhancing brown fat stores of all lambs may have important implications for lamb survival and postnatal growth.
authors.affiliation: AgResearch Grasslands, Private Bag 11008, Palmerston North, 4442 New Zealand.
authors.forename: Sue
authors.initials: S
authors.lastname: McCoard
authors.affiliation: AgResearch Grasslands, Private Bag 11008, Palmerston North, 4442 New Zealand.
authors.forename: Francisco
authors.initials: F
authors.lastname: Sales
authors.affiliation: AgResearch Grasslands, Private Bag 11008, Palmerston North, 4442 New Zealand.
authors.forename: Nina
authors.initials: N
authors.lastname: Wards
authors.affiliation: AgResearch Grasslands, Private Bag 11008, Palmerston North, 4442 New Zealand.
authors.forename: Quentin
authors.initials: Q
authors.lastname: Sciascia
authors.affiliation: Ngapouri Research Farm, Liggins Institute, University of Auckland, Auckland, New Zealand.
authors.forename: Mark
authors.initials: M
authors.lastname: Oliver
authors.affiliation: AgResearch Grasslands, Private Bag 11008, Palmerston North, 4442 New Zealand.
authors.forename: John
authors.initials: J
authors.lastname: Koolaard
authors.affiliation: AgResearch Grasslands, Private Bag 11008, Palmerston North, 4442 New Zealand.
authors.forename: Danitsja
authors.initials: D
authors.lastname: van der Linden
keywords: Amino acids; Arginine; Birth weight; Fetal; Sheep