title: A sensitive chemiluminescence imaging immunoassay for simultaneous detection of serum oxidized lipoprotein(a) and low density lipoprotein.
abstract: BACKGROUND
  Oxidized lipoprotein(a) [ox-Lp(a)] and oxidized low density lipoprotein (ox-LDL) levels have been reported to be useful predictors of cardiovascular events. The study developed a chemiluminescence (CL) imaging immunoassay method for simultaneously detecting serum concentrations of ox-Lp(a) and ox-LDL.
  
  
  METHODS
  Ox-Lp(a) and ox-LDL levels were measured by CL imaging immunoassay using a disposable immunosensor array as the carrier and a charge-coupled device as the detector, and were studied in 46 acute coronary syndromes (ACS) patients, 58 stable coronary artery disease (CAD) and 61 control subjects.
  
  
  RESULTS
  This method showed good linear relations (R²>0.99) in the concentration range of 2.00×10⁻⁵-2.00×10⁻¹ and 2.40×10⁻⁴-2.40 U/mL for ox-Lp(a) and ox-LDL, respectively. The detection limits for ox-Lp(a) and ox-LDL were 2.40×10⁻⁶ and 3.00×10⁻⁵ U/mL, respectively. The intra- and inter-assay coefficients of variation (CV) were 4.90%-6.76% and 7.11%-10.06% for ox-Lp(a), and 5.01%-6.04% and 5.47%-9.77% for ox-LDL, respectively. The mean recovery was 99.31% for ox-Lp(a) and 99.57% for ox-LDL, respectively. Significant correlations were observed between ox-Lp(a) levels detected by CL imaging immunoassay and ELISA, and between ox-LDL levels detected by the two methods, respectively. Furthermore, ox-Lp(a) and ox-LDL levels increased in stable CAD, and especially in ACS.
  
  
  CONCLUSIONS
  The CL imaging immunoassay provided a simple, sensitive and reliable method for the simultaneous determination of serum ox-Lp(a) and ox-LDL. The clinical monitoring ox-Lp(a) and ox-LDL levels may possess distinctly clinical value for assessment of CAD risk.
authors.forename: Ruijie
authors.initials: R
authors.lastname: Yu
authors.forename: Jiaxi
authors.initials: J
authors.lastname: Song
authors.forename: Jia
authors.initials: J
authors.lastname: Wu
authors.forename: Dongmei
authors.initials: D
authors.lastname: Niu
authors.forename: Lijuan
authors.initials: L
authors.lastname: Ma
authors.forename: Chen
authors.initials: C
authors.lastname: Zong
authors.forename: Huangxian
authors.initials: H
authors.lastname: Ju
authors.forename: Junjun
authors.initials: J
authors.lastname: Wang
mesh_terms: D054058:Acute Coronary Syndrome / Q000097:blood; D001774:Blood Chemical Analysis / Q000379:methods*; D002138:Calibration; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000097:blood; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D008077:Lipoproteins, LDL / Q000097:blood*; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged
chemical_list: D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein