title: SCO5745, a bifunctional RNase J ortholog, affects antibiotic production in Streptomyces coelicolor.
abstract: The bacterial RNases J are considered bifunctional RNases possessing both endo- and exonucleolytic activities. We have isolated an RNase J ortholog from Streptomyces coelicolor encoded by the gene sco5745. We overexpressed a decahistidine-tagged version of SCO5745 and purified the overexpressed protein by immobilized metal ion affinity chromatography. We demonstrated the presence of both 5'-to-3' exonucleolytic and endonucleolytic activities on the Bacillus subtilis thrS transcript. Exonucleoytic activity predominated with 5' monophosphorylated thrS, while endonucleolytic activity predominated with 5' triphosphorylated thrS. While sco5745 is the only RNase J allele in S. coelicolor, the gene is not essential. Its disruption resulted in delayed production of the antibiotic actinorhodin, overproduction of undecylprodigiosin, and diminished production of the calcium-dependent antibiotic, in comparison with the parental strain.
authors.affiliation: Department of Biology, Emory University, Atlanta, Georgia, USA.
authors.forename: Patricia
authors.initials: P
authors.lastname: Bralley
authors.forename: Madiha
authors.initials: M
authors.lastname: Aseem
authors.forename: George H
authors.initials: GH
authors.lastname: Jones
mesh_terms: D000880:Anthraquinones / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D002846:Chromatography, Affinity; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial; D055786:Gene Knockout Techniques; D006868:Hydrolysis; D011353:Prodigiosin / Q000031:analogs & derivatives / Q000096:biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012260:Ribonucleases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D048372:Streptomyces coelicolor / Q000235:genetics* / Q000378:metabolism*; D000095622:Benzoisochromanequinones
chemical_list: D000880:Anthraquinones; D000900:Anti-Bacterial Agents; D011353:Prodigiosin; D012333:RNA, Messenger; D011994:Recombinant Proteins; D012260:Ribonucleases; C047347:undecylprodigiosin; C013390:actinorhodin; D000095622:Benzoisochromanequinones