title: [Effect of Panax notoginseng saponins on cytokines in liver fibrosis rats].
abstract: OBJECTIVE
  To observe the effect of Panax notoginseng saponins (PNS) on cytokines production in liver fibrosis rats and its mechanism.
  
  
  METHODS
  SD rats were randomly divided into 6 groups, including normal group, liver fibrosis model group, PNS (50, 100, 200 mg/kg) treated groups and Col group. Rat liver fibrosis was induced by CCl4 twice a week for 18 weeks. PNS were administered daily from the 9th week for 10 weeks. The IL-1, IL-6, IL-10, TNF-alpha level in serum were determined by radioimmunity. The degree of liver fibrosis was observed by HE and immuninochemistry. The expression of TGF-beta, TNF-alpha mRNA were detected by RT-PCR technology.
  
  
  RESULTS
  Compared with the fibrotic model group, PNS (100, 200 mg/kg) could not only significantly reduce histopathological change, but also effectively decrease the level of IL-1, IL-6, NF-kappaB, TNF-alpha ,TGF-beta, and increase the level of IL-10.
  
  
  CONCLUSIONS
  PNS has protective effect on liver fibrosis and it maybe related to regulating cytokine network.
authors.affiliation: First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Grade 3 Laboratory of Traditional Chinese Medicine Preparation, State Administration of TCM, Hefei 230031, China. jhanbing@163.com
authors.forename: Hui
authors.initials: H
authors.lastname: Jiang
authors.affiliation: First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Grade 3 Laboratory of Traditional Chinese Medicine Preparation, State Administration of TCM, Hefei 230031, China.
authors.forename: Yong-Zhong
authors.initials: YZ
authors.lastname: Wang
authors.affiliation: First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Grade 3 Laboratory of Traditional Chinese Medicine Preparation, State Administration of TCM, Hefei 230031, China.
authors.forename: Xiao-Chuang
authors.initials: XC
authors.lastname: Liu
authors.affiliation: First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Grade 3 Laboratory of Traditional Chinese Medicine Preparation, State Administration of TCM, Hefei 230031, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Xue
mesh_terms: D000818:Animals; D002251:Carbon Tetrachloride; D003078:Colchicine / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D016207:Cytokines / Q000097:blood*; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008106:Liver Cirrhosis, Experimental / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism*; D008297:Male; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D053723:Panax notoginseng / Q000737:chemistry*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012503:Saponins / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000378:metabolism
chemical_list: D016207:Cytokines; D016328:NF-kappa B; D012333:RNA, Messenger; D012503:Saponins; D014409:Tumor Necrosis Factor-alpha; D002251:Carbon Tetrachloride; D003078:Colchicine