title: Leber's Hereditary Optic Neuropathy is Associated with Compound Primary Mutations of Mitochondrial ND1 m.3635G > A and ND6 m.14502 T > C.
abstract: OBJECTIVE
  To describe the clinical and molecular characteristics of a Chinese Leber hereditary optic neuropathy (LHON) pedigree with compound mitochondrial DNA (mtDNA) mutations of m.3635G > A and m.14502T > C.
  
  
  METHODS
  A total of 22 individuals (2 affected, 20 unaffected) from a five-generation Chinese family with LHON underwent comprehensive ophthalmic examination, including visual acuity, slit lamp examination, fundoscopy, visual field examination and visual evoked potentials (VEP). The complete mtDNA genome of the two patients were amplified by polymerase chain reaction, sequenced using a Bigdye terminator v3.1 cycle sequencing kit and analyzed on an ABI 3700XL Genetic Analyzer.
  
  
  RESULTS
  Two LHON patients in the family presented typical features of LHON: painless and progressive deterioration of bilateral vision, bilateral optic atrophy, centrocecal scotomata in both eyes and significant prolonged P100 latency and low amplitude potential in VEP. Compound primary mtDNA mutations of m.3635G > A and m.14502T > C were identified in these two patients and another 12 living matrilineal members of the pedigree. Haplogroup analysis showed the patients in this LHON family belonged to the N9b1 haplogroup. Modeled mutant structure showed the mutations altered the molecular local space conformation on the surface of ND1 and ND6.
  
  
  CONCLUSIONS
  Compound mtDNA mutations of m.3635G > A and m.14502T > C presented with low penetration, and the patients with these compound mutations exhibited mild visual impairment. The biological information analysis suggested that m.14502T > C might play a protective role in LHON associated with m.3635G > A. The haplogroup analysis indicated that the mtDNA haplogroup might be an important factor affecting the expression of LHON associated with m.3635G > A and/or m.14502T > C.
authors.affiliation: a Department of Ophthalmology , General Hospital of Chinese People's Liberation Army , Beijing , China .
authors.forename: Xin
authors.initials: X
authors.lastname: Jin
authors.affiliation: b Department of Biochemistry and Molecular Biology , School of Basic Medical Sciences, Fourth Military Medical University , Xi'an , China , and.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Wang
authors.affiliation: a Department of Ophthalmology , General Hospital of Chinese People's Liberation Army , Beijing , China .
authors.forename: Yan
authors.initials: Y
authors.lastname: Gong
authors.affiliation: a Department of Ophthalmology , General Hospital of Chinese People's Liberation Army , Beijing , China .
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
authors.affiliation: a Department of Ophthalmology , General Hospital of Chinese People's Liberation Army , Beijing , China .|c The Hospital of the Chinese People's Armed Police Forces , Jingzhong , China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Wang
authors.affiliation: a Department of Ophthalmology , General Hospital of Chinese People's Liberation Army , Beijing , China .
authors.forename: Tingjun
authors.initials: T
authors.lastname: Chen
authors.affiliation: a Department of Ophthalmology , General Hospital of Chinese People's Liberation Army , Beijing , China .
authors.forename: Shihui
authors.initials: S
authors.lastname: Wei
mesh_terms: D000293:Adolescent; D044466:Asian People / Q000235:genetics; D002681:China; D004272:DNA, Mitochondrial / Q000235:genetics*; D005074:Evoked Potentials, Visual; D005820:Genetic Testing; D054629:Genome, Mitochondrial; D006801:Humans; D008297:Male; D009154:Mutation; D009245:NADH Dehydrogenase / Q000235:genetics*; D029242:Optic Atrophy, Hereditary, Leber / Q000235:genetics*; D010375:Pedigree; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D014792:Visual Acuity
chemical_list: D004272:DNA, Mitochondrial; C507225:MT-ND6 protein, human; D009245:NADH Dehydrogenase; C515075:NADH dehydrogenase subunit 1, human
keywords: LHON; m.14502T > C; m.3635G > A; mtDNA; mutation