title: Synthesis of peptides of Carapax Trionycis and their inhibitory effects on TGF-β1-induced hepatic stellate cells.
abstract: We previous identified the antifibrotic active ingredients from Carapax Trionycis as two peptides. Here, we synthesized these two peptides (peptide 1 and peptide 2) by a solid phase method and examined their effects on proliferation and activation of cultured hepatic stellate cells (HSC) which are the main ECM (extracellular matrix)-producing cells in fibrosis progression. We demonstrated that peptide 1 and peptide 2 significantly reduced HSC proliferation and activation in a dose dependent manner. Further, peptide 1 and peptide 2 could interfere with TGF-signaling by down-regulating Smad 3 phosphorylation. Thus, these synthetic peptides of Carapax Trionycis could inhibit proliferation and activation of HSC and might be used as a candidate for treatment of liver fibrosis.
authors.affiliation: School of Pharmacy, Hubei University of Traditional Chinese Medicine, Hubei, China.
authors.forename: C L
authors.initials: CL
authors.lastname: Hu
authors.forename: X Z
authors.initials: XZ
authors.lastname: Peng
authors.forename: Y P
authors.initials: YP
authors.lastname: Tang
authors.forename: Y W
authors.initials: YW
authors.lastname: Liu
mesh_terms: D060105:Animal Shells; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000378:metabolism; D055166:Hepatic Stellate Cells / Q000187:drug effects*; D006801:Humans; D008516:Medicine, Chinese Traditional; D030162:Reptilian Proteins / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1 / Q000037:antagonists & inhibitors*; D014426:Turtles
chemical_list: D030162:Reptilian Proteins; D053773:Transforming Growth Factor beta1