title: Cytochemical localization of glycolate oxidase in microbodies of Klebsormidium.
abstract: The filamentous green alga Klebsormidium flaccidum A.Br. was fixed with glutaraldehyde, incubated in a cytochemical medium designed to detect glycolate-oxidase activity, and prepared for electron microscopy. Heavy deposits of stain were observed in microbodies following incubation with either glycolate or L-lactate as substrate, but not after incubation with D-lactate or H2O. When Chlamydomanas reinhardi Dangeared cells were treated in the same way, their microbodies did not appear stained. The results establish that in Klebsormidium glycolate-oxidase occurs in microbodies (peroxisomes), as it does in angiosperms; also, they emphasize the dichotomy between those green algae which contain glycolate-oxidase and those, such as Chlamydomonas, which possess the mitochondrial enzyme glycolate dehydrogenase.
authors.affiliation: Department of Biological Sciences, Mount Holyoke College, 01075, South Hadley, MA, USA.
authors.forename: P J
authors.initials: PJ
authors.lastname: Gruber
authors.forename: S E
authors.initials: SE
authors.lastname: Frederick