title: Interaction of CSFV E2 protein with swine host factors as detected by yeast two-hybrid system.
abstract: E2 is one of the envelope glycoproteins of pestiviruses, including classical swine fever virus (CSFV) and bovine viral diarrhea virus (BVDV). E2 is involved in several critical functions, including virus entry into target cells, induction of a protective immune response and virulence in swine. However, there is no information regarding any host binding partners for the E2 proteins. Here, we utilized the yeast two-hybrid system and identified fifty-seven host proteins as positive binding partners which bound E2 from both CSFV and BVDV with the exception of two proteins that were found to be positive for binding only to CSFV E2. Alanine scanning of CSFV E2 demonstrated that the binding sites for these cellular proteins on E2 are likely non-linear binding sites. The possible roles of the identified host proteins are discussed as the results presented here will be important for future studies to elucidate mechanisms of host protein-virus interactions during pestivirus infection. However, due to the limitations of the yeast two hybrid system, the proteins identified is not exhaustive and each interaction identified needs to be confirmed by independent experimental approaches in the context of virus-infected cells before any definitive conclusion can be drawn on relevance for the virus life cycle.
authors.affiliation: Plum Island Animal Disease Center, Agriculture Research Service, United States Department of Agriculture, Greenport, New York, United States of America ; Department of Pathobiology and Veterinary Science, University of Connecticut, Storrs, Connecticut, United States of America.
authors.forename: Douglas P
authors.initials: DP
authors.lastname: Gladue
authors.affiliation: Plum Island Animal Disease Center, Agriculture Research Service, United States Department of Agriculture, Greenport, New York, United States of America.
authors.forename: Ryan
authors.initials: R
authors.lastname: Baker-Bransetter
authors.affiliation: Plum Island Animal Disease Center, Agriculture Research Service, United States Department of Agriculture, Greenport, New York, United States of America.
authors.forename: Lauren G
authors.initials: LG
authors.lastname: Holinka
authors.affiliation: Plum Island Animal Disease Center, Agriculture Research Service, United States Department of Agriculture, Greenport, New York, United States of America.
authors.forename: Ignacio J
authors.initials: IJ
authors.lastname: Fernandez-Sainz
authors.affiliation: Plum Island Animal Disease Center, Agriculture Research Service, United States Department of Agriculture, Greenport, New York, United States of America ; Department of Pathobiology and Veterinary Science, University of Connecticut, Storrs, Connecticut, United States of America.
authors.forename: Vivian
authors.initials: V
authors.lastname: O'Donnell
authors.affiliation: Plum Island Animal Disease Center, Agriculture Research Service, United States Department of Agriculture, Greenport, New York, United States of America.
authors.forename: Paige
authors.initials: P
authors.lastname: Fletcher
authors.affiliation: Plum Island Animal Disease Center, Department of Homeland Security, Greenport, New York, United States of America.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Plum Island Animal Disease Center, Agriculture Research Service, United States Department of Agriculture, Greenport, New York, United States of America.
authors.forename: Manuel V
authors.initials: MV
authors.lastname: Borca
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000821:virology; D002417:Cattle; D006691:Classical Swine Fever / Q000821:virology; D006692:Classical Swine Fever Virus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001908:Diarrhea Viruses, Bovine Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D015723:Gene Library; D054884:Host-Pathogen Interactions; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D011485:Protein Binding; D016415:Sequence Alignment; D013552:Swine; D020798:Two-Hybrid System Techniques; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D014759:Viral Envelope Proteins; C087086:glycoprotein E2, classical swine fever virus