title: β-Glucan synthetase activity in Golgi vesicles ofPetunia hybrida.
abstract: β-Glucan synthetase activity has been demonstrated in a Golgi vesicle fraction isolated from pollen tubes ofPetunia hybrida. Thisβ-glucan synthetase activity differs from that of most other higher plants in its inability to incorporate [(14)C]glucose from GDP-[(14)C]glucose. UDP-[(14)C]glucose, however, is an appropriate glucose donor for this enzyme. The optimum conditions for thisβ-glucan synthetase activity are: 1 mg Golgi vesicle protein/ml reaction mixture; pH=±8 and a temperature of 25°C. The newly synthesized alkali-insoluble glucan containsβ-1,3- as well as β-1,4-glucosidic linkages.
authors.affiliation: Department of Botany, University of Nijmegen, Toernooiveld, Nijmegen, The Netherlands.
authors.forename: J P
authors.initials: JP
authors.lastname: Helsper
authors.forename: J H
authors.initials: JH
authors.lastname: Veerkamp
authors.forename: M M
authors.initials: MM
authors.lastname: Sassen