title: Plant tRNA nucleotidyltransferase : I. Isolation and purification of tRNA nucleotidyltransferase from Lupinus luteus seeds.
abstract: A method for purification of tRNA nucleotidyltransferase from Lupinus luteus L. seeds on a preparative scale is presented. This involved ammonium sulfate fractionation and chromatography on DEAE-cellulose, hydroxylapatite, and QAE-Sephadex A-50. The final enzyme prepartion was apparently homogeneous and a 2000-fold purification was achieved. Data presented suggest the existence of only one form of tRNA nucleotidyltransferase in dry lupin seeds.
authors.affiliation: Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 30/36, 60-479, Poznań, Poland.
authors.forename: H
authors.initials: H
authors.lastname: Cudny
authors.forename: M
authors.initials: M
authors.lastname: Pietrzak
authors.forename: J
authors.initials: J
authors.lastname: Kaczkowski