title: Volatile profile, phytochemicals and antioxidant activity of virgin olive oils from Croatian autochthonous varieties Mašnjača and Krvavica in comparison with Italian variety Leccino.
abstract: Virgin olive oils (VOOs) obtained from the fruits of Croatian autochthonous varieties Mašnjača and Krvavica were extensively characterized for the first time. Investigated oils were compared with the oil obtained from Italian variety Leccino, grown and processed under the same conditions. Headspace volatile profile, tocopherols, chlorophylls, carotenoids and total phenolic content, peroxide value, % acidity, K232, K270 as well as antioxidant activity (DPPH) of the oils' hydrophilic fractions (HFs) including their phenolic composition were assessed by means of HS-SPME/GC-MS, HPLC-FL, HPLC-DAD and spectrophotometric methods, respectively. Most of the studied quality parameters varied between the cultivars. The main volatile compounds detected in all tested olive oils were the C6 compounds derived from polyunsaturated fatty acids through the lipoxygenase pathway. Krvavica oil was characterized by hexanal (8.8%-9.4%). Leccino oil contained the highest percentage of (E)-hex-2-enal (73.4%-74.0%), whereas (Z)-hex-3-enal (21.9%-25.0%) and (E)-hex-2-enal (27.6%-28.9%) dominated in Mašnjača oil. Leccino oil contained the highest amount of tocopherols (312.4 mg/kg), chlorophylls (7.3 mg/kg), carotenoids (4.2 mg/kg) and total phenols (246.6 mg/kg). The HF of Leccino oil showed the highest antioxidant capacity (1.3 mmol TEAC/kg), while the HFs of Mašnjača and Krvavica oils exhibited the activity of 0.5 mmol TEAC/kg.
authors.affiliation: Department of Food Technology, Marko Marulić Polytechnic in Knin, Petra Krešimira IV, 30, Knin 22300, Croatia. msarolic@veleknin.hr.
authors.forename: Mladenka
authors.initials: M
authors.lastname: Sarolić
authors.affiliation: Department of Food Technology, Marko Marulić Polytechnic in Knin, Petra Krešimira IV, 30, Knin 22300, Croatia. mgugic@veleknin.hr.
authors.forename: Mirko
authors.initials: M
authors.lastname: Gugić
authors.affiliation: Department of Food Technology, Marko Marulić Polytechnic in Knin, Petra Krešimira IV, 30, Knin 22300, Croatia. tuberoso@unica.it.
authors.forename: Carlo Ignazio Giovanni
authors.initials: CI
authors.lastname: Tuberoso
authors.affiliation: Department of Food Technology, Marko Marulić Polytechnic in Knin, Petra Krešimira IV, 30, Knin 22300, Croatia. igor@ktf-split.hr.
authors.forename: Igor
authors.initials: I
authors.lastname: Jerković
authors.affiliation: Department of Food Technology, Marko Marulić Polytechnic in Knin, Petra Krešimira IV, 30, Knin 22300, Croatia. msuste@veleknin.hr.
authors.forename: Marko
authors.initials: M
authors.lastname: Suste
authors.affiliation: Department of Food Technology, Marko Marulić Polytechnic in Knin, Petra Krešimira IV, 30, Knin 22300, Croatia. zmarijanovic@veleknin.hr.
authors.forename: Zvonimir
authors.initials: Z
authors.lastname: Marijanović
authors.affiliation: Department of Food Technology, Marko Marulić Polytechnic in Knin, Petra Krešimira IV, 30, Knin 22300, Croatia. kus.piotrek@gmail.com.
authors.forename: Piotr Marek
authors.initials: PM
authors.lastname: Kuś
mesh_terms: D001713:Biphenyl Compounds / Q000737:chemistry; D002338:Carotenoids / Q000737:chemistry / Q000302:isolation & purification; D002734:Chlorophyll / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D017523:Croatia; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification; D005609:Free Radicals / Q000737:chemistry; D007558:Italy; D057230:Limit of Detection; D009822:Oils, Volatile / Q000737:chemistry* / Q000302:isolation & purification; D031658:Olea / Q000737:chemistry*; D000069463:Olive Oil; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification; D064209:Phytochemicals / Q000737:chemistry / Q000302:isolation & purification; D010851:Picrates / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D024505:Tocopherols / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005609:Free Radicals; D009822:Oils, Volatile; D000069463:Olive Oil; D010636:Phenols; D064209:Phytochemicals; D010851:Picrates; D010938:Plant Oils; D002734:Chlorophyll; D002338:Carotenoids; C004931:1,1-diphenyl-2-picrylhydrazyl; D024505:Tocopherols