title: Use of halogenated precursors to define a transcription time window after treatment with hypometabolizing molecules.
abstract: A new method is proposed which combines the high spatial resolution of transmission electron microscopy with information on the dynamics of transcription. Incorporation of two different RNA precursors was used to define a time transcription window on cultured cells treated with hypometabolizing peptides which are known to modulate transcription. This procedure allows detecting a single fibril of newly synthesized RNA in the time range in which it is transcribed.
authors.affiliation: Dipartimento di Biologia e Biotecnologie, Laboratorio di Biologia Cellulare e Neurobiologia, Università degli Studi di Pavia, Via Ferrata 1, Pavia, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Spedito
authors.forename: Barbara
authors.initials: B
authors.lastname: Cisterna
authors.forename: Manuela
authors.initials: M
authors.lastname: Malatesta
authors.forename: Marco
authors.initials: M
authors.lastname: Biggiogera
mesh_terms: D045744:Cell Line, Tumor; D002467:Cell Nucleus; D049109:Cell Proliferation; D003609:Dactinomycin / Q000494:pharmacology; D004004:Dichlororibofuranosylbenzimidazole / Q000494:pharmacology; D016308:Enkephalin, Leucine-2-Alanine / Q000031:analogs & derivatives / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D046529:Microscopy, Electron, Transmission / Q000379:methods; D012322:RNA Precursors / Q000737:chemistry* / Q000235:genetics; D012335:RNA, Ribosomal / Q000096:biosynthesis / Q000235:genetics*; D013194:Staining and Labeling / Q000379:methods; D014158:Transcription, Genetic / Q000187:drug effects / Q000235:genetics*; D014529:Uridine / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D012322:RNA Precursors; D012335:RNA, Ribosomal; D003609:Dactinomycin; D004004:Dichlororibofuranosylbenzimidazole; D016308:Enkephalin, Leucine-2-Alanine; D014529:Uridine