title: Characterization by Suppression Subtractive Hybridization of Transcripts That Are Differentially Expressed in Leaves of Anthracnose-Resistant Ramie Cultivar.
abstract: For the purpose of screening putative anthracnose resistance-related genes of ramie (Boehmeria niveaL. Gaud), a cDNA library was constructed by suppression subtractive hybridization using anthracnose-resistant cultivar Huazhu no. 4. The cDNAs from Huazhu no. 4, which were infected withColletotrichum gloeosporioides, were used as the tester and cDNAs from uninfected Huazhu no. 4 as the driver. Sequencing analysis and homology searching showed that these clones represented 132 single genes, which were assigned to functional categories, including 14 putative cellular functions, according to categories established forArabidopsis. These 132 genes included 35 disease resistance and stress tolerance-related genes including putative heat-shock protein 90, metallothionein, PR-1.2 protein, catalase gene, WRKY family genes, and proteinase inhibitor-like protein. Partial disease-related genes were further analyzed by reverse transcription PCR and RNA gel blot. These expressed sequence tags are the first anthracnose resistance-related expressed sequence tags reported in ramie.
authors.forename: Wang
authors.initials: W
authors.lastname: Xuxia
authors.forename: Chen
authors.initials: C
authors.lastname: Jie
authors.forename: Wang
authors.initials: W
authors.lastname: Bo
authors.forename: Liu
authors.initials: L
authors.lastname: Lijun
authors.forename: Jiang
authors.initials: J
authors.lastname: Hui
authors.forename: Tang
authors.initials: T
authors.lastname: Diluo
authors.forename: Peng
authors.initials: P
authors.lastname: Dingxiang
keywords: Anthracnose; Boehmeria nivea L. Gaud; Pathogenesis-related genes; Suppression subtractive hybridization (SSH)