title: Multiplex PCR assay using SCAR primers to detect Eimeria spp. in chicken.
abstract: About 11 faecal samples from various regions of Tamil Nadu and Andhra Pradesh containing mixed spectrum of Eimeria species detected by morphometry viz. 15.4 × 11.2, 28.5 × 20.3, 31.1 × 18.5, 13.2 × 12.4, 20.8 × 17.5 and 22 × 18 μm for E. acervulina, E. brunetti, E. maxima, E. mitis, E. necatrix, E. praecox and E. tenella respectively were taken for the study. The oocysts were concentrated and purified using faecal harvest protocol. The genomic DNA is extracted as per kit protocol. The amplicons of sizes 539 bp (E. tenella) and 460 bp (E. mitis) obtained could be visualised in a single lane for the multiplex PCR assay using sequence characterized amplified region primers.
authors.affiliation: Department of Veterinary Parasitology, Madras Veterinary College, Tamil Nadu Veterinary & Animal Sciences University, Vepery, Chennai, 600 007 India ; Department of Veterinary Parasitology, College of Veterinary Science, Sri Venkateswara Veterinary University, Korutla, 505 326 Andhra Pradesh India.
authors.forename: P
authors.initials: P
authors.lastname: Venkateswara Rao
authors.affiliation: Department of Veterinary Parasitology, Madras Veterinary College, Tamil Nadu Veterinary & Animal Sciences University, Vepery, Chennai, 600 007 India.
authors.forename: M
authors.initials: M
authors.lastname: Raman
authors.affiliation: Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary & Animal Sciences University, Vepery, Chennai, 600 007 India.
authors.forename: G
authors.initials: G
authors.lastname: Dhinakarraj
authors.affiliation: Department of Veterinary Parasitology, Madras Veterinary College, Tamil Nadu Veterinary & Animal Sciences University, Vepery, Chennai, 600 007 India.
authors.forename: S
authors.initials: S
authors.lastname: Abdul Basith
authors.affiliation: Department of Veterinary Parasitology, Madras Veterinary College, Tamil Nadu Veterinary & Animal Sciences University, Vepery, Chennai, 600 007 India.
authors.forename: S
authors.initials: S
authors.lastname: Gomathinayagam
keywords: Eimeria; Faecal harvest; Mixed spectrum; Morphometry; Multiplex PCR; SCAR