title: Rapid 3D fluorescence imaging of individual optically trapped living immune cells.
abstract: We demonstrate an approach to rapidly characterize living suspension cells in 4 dimensions while they are immobilized and manipulated within optical traps. A single, high numerical aperture objective lens is used to separate the imaging plane from the trapping plane. This facilitates full control over the position and orientation of multiple trapped cells using a spatial light modulator, including directed motion and object rotation, while also allowing rapid 4D imaging. This system is particularly useful in the handling and investigation of the behavior of non-adherent immune cells. We demonstrate these capabilities by imaging and manipulating living, fluorescently stained Jurkat T cells.
authors.affiliation: NSF Center for Biophotonics Science and Technology, University of California, Davis, Sacramento, CA, 95817, USA.
authors.forename: Deanna
authors.initials: D
authors.lastname: Wolfson
authors.forename: Michael
authors.initials: M
authors.lastname: Steck
authors.forename: Martin
authors.initials: M
authors.lastname: Persson
authors.forename: Gregory
authors.initials: G
authors.lastname: McNerney
authors.forename: Ana
authors.initials: A
authors.lastname: Popovich
authors.forename: Mattias
authors.initials: M
authors.lastname: Goksör
authors.forename: Thomas
authors.initials: T
authors.lastname: Huser
mesh_terms: D002138:Calibration; D002470:Cell Survival; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D019169:Jurkat Cells; D061848:Optical Imaging / Q000379:methods*; D052898:Optical Tweezers; D013601:T-Lymphocytes / Q000166:cytology*; D013997:Time Factors
keywords: 3-D imaging; Jurkat cells; confocal microscopy; fluorescence microscopy; optical tweezers