title: Imaging of Mohs micrographic surgery sections using full-field optical coherence tomography: a pilot study.
abstract: BACKGROUND
  Full-field optical coherence tomography (FF-OCT) is a new noninvasive imaging technique that can see down to the cellular level without tissue preparation or contrast agents.
  
  
  OBJECTIVE
  To use FF-OCT to image Mohs micrographic surgery specimens and verify the ability of a dermatopathologist to identify or exclude malignancy.
  
  
  METHODS
  Two Mohs surgeons supplied 18 Mohs sections from 11 patients. Each section was scanned using the FF-OCT, and a dermatopathologist blinded to the diagnosis examined the images for malignancy. The FF-OCT images were then compared with the intraoperative hematoxylin and eosin (H&E)-stained frozen sections for concordance.
  
  
  RESULTS
  All 9 FF-OCT images interpreted as negative for malignancy were in agreement with the H&E frozen sections. Six of the remaining FF-OCT images were correctly interpreted as positive for malignancy, and three were deferred because malignancy could not be confirmed or excluded.
  
  
  CONCLUSIONS
  Malignancy in Mohs sections can correctly be identified or excluded using FF-OCT. Although not ready for clinical use in its current state, FF-OCT has the potential to be incorporated into the Mohs workflow in the future.
authors.affiliation: School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania.
authors.forename: John R
authors.initials: JR
authors.lastname: Durkin
authors.forename: Jeffery L
authors.initials: JL
authors.lastname: Fine
authors.forename: Hakeem
authors.initials: H
authors.lastname: Sam
authors.forename: Melissa
authors.initials: M
authors.lastname: Pugliano-Mauro
authors.forename: Jonhan
authors.initials: J
authors.lastname: Ho
mesh_terms: D002280:Basal Cell Carcinoma / Q000473:pathology / Q000601:surgery; D002281:Carcinoma, Basosquamous / Q000473:pathology / Q000601:surgery; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000601:surgery; D004867:Equipment Design; D005260:Female; D005629:Frozen Sections; D006801:Humans; D008297:Male; D015580:Mohs Surgery; D018365:Neoplasm, Residual / Q000473:pathology; D010865:Pilot Projects; D012878:Skin Neoplasms / Q000473:pathology* / Q000601:surgery*; D041623:Tomography, Optical Coherence / Q000295:instrumentation*