title: CagA subtyping in Helicobacter pylori isolates from gastric cancer patients in an ethnic Kashmiri population.
abstract: The association between gastric cancer and Helicobacter pylori has been well established. Among H. pylori virulence genes the most important determinant is the cytotoxin associated antigen gene (cagA) which is characterized by the presence of repeated EPIYA motifs at the C terminus of the protein. From the alignment and number of these EPIYA motifs, two major types of CagA protein have been identified.
  
  
  OBJECTIVE
  The aim of this study was to classify the CagA into eastern or western type and to determine the number and type of motifs present.
  
  
  METHODS
  The CagA subtyping was done by PCR and multiplex PCR for eastern/western classification and determination of EPIYA motifs respectively.
  
  
  RESULTS
  All the isolates studied were of the western type, with 70% of the isolates having more than one EPIYA-C motifs. No statistically significant association was found between the presence of CagA and more than one EPIYA-C motifs with the clinical outcome (differentiation status of the tumour).
authors.affiliation: Dept. of Immunology and Molecular Medicine, Sher-i-Kashmir Institute of Medical Sciences (SKIMS), Soura, Srinagar, Kashmir, J&K 190011, India.
authors.forename: Qurteeba
authors.initials: Q
authors.lastname: Qadri
authors.affiliation: Dept. of Immunology and Molecular Medicine, Sher-i-Kashmir Institute of Medical Sciences (SKIMS), Soura, Srinagar, Kashmir, J&K 190011, India.
authors.forename: Dil
authors.initials: D
authors.lastname: Afroze
authors.affiliation: Dept. of Immunology and Molecular Medicine, Sher-i-Kashmir Institute of Medical Sciences (SKIMS), Soura, Srinagar, Kashmir, J&K 190011, India.
authors.forename: Roohi
authors.initials: R
authors.lastname: Rasool
authors.affiliation: Dept. of Gastroenterology, Sher-i-Kashmir Institute of Medical Sciences (SKIMS), Soura, Kashmir, J&K 190011, India.
authors.forename: G M
authors.initials: GM
authors.lastname: Gulzar
authors.affiliation: Dept. of General Surgery, Sher-i-Kashmir Institute of Medical Sciences (SKIMS), Soura, Kashmir, J&K 190011, India.
authors.forename: Sameer
authors.initials: S
authors.lastname: Naqash
authors.affiliation: Dept. of Immunology and Molecular Medicine, Sher-i-Kashmir Institute of Medical Sciences (SKIMS), Soura, Srinagar, Kashmir, J&K 190011, India.
authors.forename: Mushtaq A
authors.initials: MA
authors.lastname: Siddiqi
authors.affiliation: Dept. of Immunology and Molecular Medicine, Sher-i-Kashmir Institute of Medical Sciences (SKIMS), Soura, Srinagar, Kashmir, J&K 190011, India. Electronic address: zaffaramin@gmail.com.
authors.forename: Zafar A
authors.initials: ZA
authors.lastname: Shah
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005756:Gastritis / Q000208:ethnology / Q000382:microbiology*; D005838:Genotype; D016481:Helicobacter Infections / Q000208:ethnology / Q000382:microbiology*; D016480:Helicobacter pylori / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007194:India / Q000453:epidemiology; D010641:Phenotype; D016133:Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000208:ethnology / Q000382:microbiology*; D037521:Virulence Factors / Q000235:genetics
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D037521:Virulence Factors; C080866:cagA protein, Helicobacter pylori
keywords: CagA protein; EPIYA motifs; Gastric cancer; Helicobacter pylori