title: Induction of sperm impairments in mice as a sensitive biomarker of arsenic toxicity.
abstract: The ubiquitous presence of arsenic (a toxic metalloid) in our environment, particularly in our drinking water, is a serious health hazard of global concern. The present work deals with the assessment of arsenic toxicity through the analysis of induced sperm impairments in sperm head morphology and sperm count in mice at low exposures compared to the magnitude of response at high exposure levels. The animals were exposed to four doses of arsenic, ranging from lowest dose of 0.3 μg kg(-1) day(-1) (the human reference dose) to higher dose of 30 μg kg(-1) day(-1) for 15 consecutive days. The epididymal sperms were harvested after one spermatogenic cycle on the 36th day and were scored for the presence of any abnormality in their head morphology as well as changes in their count. Exposure to arsenic significantly induced, in a dose-dependent manner, increases in the frequency of sperms with abnormal head morphology from 5.12 % in control to 9.23 % in lowest dose group and 23.02 % in highest dose group. In contrast, the mean sperm counts in the epididymal wash were decreased from 6.05 million per milliliter in the control to 4.95 million per milliliter in the lowest dose group and 3.07 million in the highest dose group. The analysis of sperm impairments in mice was, therefore, found to be a highly sensitive assay to assess arsenic toxicity, exhibiting a marked male reprotoxic effect of arsenic even at its low exposure levels including the human reference dose.
authors.affiliation: Toxicogenetics Laboratory, Department of Zoology, Patna University, Patna, 800 005, India.
authors.forename: Vibudh P
authors.initials: VP
authors.lastname: Kesari
authors.forename: Amod
authors.initials: A
authors.lastname: Kumar
authors.forename: Parimal K
authors.initials: PK
authors.lastname: Khan
mesh_terms: D000818:Animals; D001151:Arsenic / Q000633:toxicity*; D015415:Biomarkers / Q000378:metabolism; D060766:Drinking Water / Q000737:chemistry; D015386:Hazardous Substances / Q000633:toxicity*; D006801:Humans; D008297:Male; D051379:Mice; D013094:Spermatozoa / Q000187:drug effects*
chemical_list: D015415:Biomarkers; D060766:Drinking Water; D015386:Hazardous Substances; D001151:Arsenic