title: Expression of PPAR-γ in adipose tissue of rats with polycystic ovary syndrome induced by DHEA.
abstract: The objective of this study was to investigate the expression of peroxisome proliferator-activated receptor-γ (PPAR-γ) in adipose tissue of the rat model of polycystic ovary syndrome (PCOS), induced by dehydroepiandrosterone (DHEA). Sixteen sexually immature Sprague-Dawley female rats were randomly assigned to the DHEA (n=8) or control (n=8) group. Adipose tissue was collected from the two rat groups following subcutaneous injection of DHEA in the DHEA group and a standard laboratory diet in the control group for 20 consecutive days. Reverse transcription polymerase chain reaction and western blot analysis were performed to detect expression of PPAR-γ at the mRNA and protein level in the adipose tissue. Both PPAR-γ mRNA and protein levels were decreased in the adipose tissue of DHEA‑induced PCOS rats compared to the control group. This decrease was significant (P<0.01). These results suggest that the pathogenesis of PCOS, which shares a number of common features with hyperandrogenemia, may involve the lipid metabolism pathway through inhibition of PPAR-γ.
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong 510630, P.R. China.
authors.forename: Yu-Xia
authors.initials: YX
authors.lastname: Wang
authors.affiliation: Department of Developmental and Regenerative Biology, College of Life Science and Technology, Jinan University, Guangzhou, Guangdong 510632, P.R. China.
authors.forename: Wei-Jie
authors.initials: WJ
authors.lastname: Zhu
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong 510630, P.R. China.
authors.forename: Bao-Guo
authors.initials: BG
authors.lastname: Xie
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D003687:Dehydroepiandrosterone; D004195:Disease Models, Animal; D005260:Female; D005786:Gene Expression Regulation; D017588:Hyperandrogenism / Q000378:metabolism / Q000473:pathology; D007279:Injections, Subcutaneous; D047495:PPAR gamma / Q000235:genetics* / Q000378:metabolism; D011085:Polycystic Ovary Syndrome / Q000139:chemically induced* / Q000235:genetics* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D047495:PPAR gamma; D012333:RNA, Messenger; D003687:Dehydroepiandrosterone