title: Phenylalanine ammonia lyase fromSporidiobolus pararoseus andRhodosporidium toruloides: Application for phenylalanine and tyrosine deamination.
abstract: Simultaneous depletion of phenylalanine and tyrosine by phenylalanine ammonia lyase is described in a mutual competitive inhibition model. The enzymes obtained fromSporidiobolus pararoseus andRhodosporidium toruloides were charaterized in terms of stability, optimal reaction parameters and kinetic behaviour. Both enzymes followed Michaelis-Menten kinetics with respect to the two amino acids. However, the enzyme fromRhodosporidium toruloides was inhibited by high tyrosine concentrations.
authors.affiliation: the Departmento de Alimentos y Biotecnologia, Facultad de Quimica, UNAM, Cd. Universitaria, 04510, Mexico City, Mexico.
authors.forename: S
authors.initials: S
authors.lastname: Kawasaki Watanabe
authors.forename: G
authors.initials: G
authors.lastname: Hernandez-Velazco
authors.forename: F
authors.initials: F
authors.lastname: Iturbe-Chiñas
authors.forename: A
authors.initials: A
authors.lastname: Lopez-Munguia