title: [Bone marrow-derived mesenchymal stem cells from estrogen deficiency induced osteoporosis mice regulate T cell migration and apoptosis through expressing MCP-1].
abstract: OBJECTIVE
  To reveal the role of bone marrow-derived mesenchymal stem cells (BMSCs) in the development of osteoporosis by comparing the differences in monocyte chemoattractant protein-1 (MCP-1) expression and T cells' migration and apoptosis induced by BMSCs from ovariectomy (OVX) group and sham group.
  
  
  METHODS
  OVX was performed on C57BL/6 mice to establish the animal models of osteoporosis. Osteoporosis was confirmed by micro-CT. The expression of MCP-1 between OVX group and sham group was examined by ELISA; after exogenous estrogen of different concentrations were given to stimulate BMSCs from OVX group, the expression of MCP-1 was observed again by ELISA. Through co-culturing of BMSCs and T cells, the change of T cells' migration and apoptosis capacity induced by BMSCs was compared between OVX group and sham group. And also, we observed the effects of exogenous estrogen of different concentrations on the T cells' migration and apoptosis capacity.
  
  
  RESULTS
  In animal models of osteoporosis induced by estrogen deficiency, BMSCs had a declined inducing effect on the capacity of T cell migration and apoptosis and expressed a decreased level of migration-related gene MCP-1. After the stimulation of estrogen of certain concentration, the declining tendency was revised to some extent.
  
  
  CONCLUSIONS
  Through expressing MCP-1, BMSCs could regulate the capacity of T cell migration and apoptosis, thus leading to the development of osteoporosis.
authors.affiliation: Department of Endodontics, Affiliated Hospital of Stomatology, Chongqing Medical University; Chongqing Key Laboratory of Oral Diseases and Biomedical Science, Chongqing 400015, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Endodontics, Affiliated Hospital of Stomatology, Chongqing Medical University; Chongqing Key Laboratory of Oral Diseases and Biomedical Science, Chongqing 400015, China.
authors.forename: Bingyi
authors.initials: B
authors.lastname: Shao
authors.affiliation: Department of Orthodontics, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Fengqing
authors.initials: F
authors.lastname: Shang
authors.affiliation: Department of Pediatric Dentistry, Affiliated Hospital of Stomatology, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Zhifei
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Orthodontics, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Oral Histology and Pathology, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Shuai
authors.affiliation: Department of Oral Histology and Pathology, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liao
authors.affiliation: Department of Oral Histology and Pathology, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Department of Endodontics, Affiliated Hospital of Stomatology, Chongqing Medical University; Chongqing Key Laboratory of Oral Diseases and Biomedical Science, Chongqing 400015, China.
authors.forename: Deqin
authors.initials: D
authors.lastname: Yang
mesh_terms: D000818:Animals; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000166:cytology; D002465:Cell Movement; D018932:Chemokine CCL2 / Q000378:metabolism*; D004967:Estrogens / Q000172:deficiency*; D005260:Female; D005786:Gene Expression Regulation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010024:Osteoporosis / Q000276:immunology / Q000378:metabolism*; D010052:Ovariectomy; D013601:T-Lymphocytes / Q000166:cytology*
chemical_list: C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D004967:Estrogens