title: Exploitation of fermented shrimp-shells hydrolysate as functional food: assessment of antioxidant, hypocholesterolemic and prebiotic activities.
abstract: In the present study the bioactivities of chitooligosaccharides of fermented shrimp-shell hydrolysate (SSH) in respect to hypocholesterolemic, antioxidant and prebiotic activity were tested in male albino rat. Rats were treated with four different diets, viz., (i) cholesterol-rich (5%) basal diet (ChB), (ii) ChB+10% chitin, (iii) ChB+10% SSH and (iv) control group (without cholesterol). After 4 weeks of treatment, body mass index, liver weight, serum total cholesterol and LDL-cholesterol in groups (ii) and (iii) were decreased significantly than group (i). SSH supplementation significantly resists oxidative stress by reducing the thiobarbituric acid reactive substances and by increasing catalase, superoxide dismutase and free radical scavenging activity. The colonization of Lactobacillus and Bifidobacterium population in small and large intestine were more in group (iii) than other groups. Reduction of Clostridium perfringens population and non-significant changes of E. coli was also noted in SSH supplement group. Histological study revealed that the villus height and villus:crypt of the small intestine were increased significantly in SSH supplemented group (iii) without any diarrheal symptoms. The results demonstrated that the shrimp-shells hydrolysate has hypocholesterolemic effect, can resist lipid peroxidation and can influence the growth of health beneficial microbes, hence can be used as functional food for hypercholesterolemic patients.
authors.affiliation: Department of Microbiology, Vidyasagar University, Midnapore 721 102, India.
authors.forename: Suman Kumar
authors.initials: SK
authors.lastname: Halder
authors.affiliation: Department of Microbiology, Vidyasagar University, Midnapore 721 102, India.
authors.forename: Atanu
authors.initials: A
authors.lastname: Adak
authors.affiliation: Department of Microbiology, Vidyasagar University, Midnapore 721 102, India.
authors.forename: Chiranjit
authors.initials: C
authors.lastname: Maity
authors.affiliation: Department of Microbiology, Vidyasagar University, Midnapore 721 102, India.
authors.forename: Arijit
authors.initials: A
authors.lastname: Jana
authors.affiliation: Department of Microbiology, Vidyasagar University, Midnapore 721 102, India.
authors.forename: Arpan
authors.initials: A
authors.lastname: Das
authors.affiliation: Department of Microbiology, Vidyasagar University, Midnapore 721 102, India.
authors.forename: Tanmay
authors.initials: T
authors.lastname: Paul
authors.affiliation: Department of Microbiology, Vidyasagar University, Midnapore 721 102, India.
authors.forename: Kuntal
authors.initials: K
authors.lastname: Ghosh
authors.affiliation: Department of Microbiology, Vidyasagar University, Midnapore 721 102, India.
authors.forename: Pradeep Kumar
authors.initials: PK
authors.lastname: Das Mohapatra
authors.affiliation: Department of Microbiology, Vidyasagar University, Midnapore 721 102, India.
authors.forename: Bikas Ranjan
authors.initials: BR
authors.lastname: Pati
authors.affiliation: Department of Microbiology, Vidyasagar University, Midnapore 721 102, India.
authors.forename: Keshab Chandra
authors.initials: KC
authors.lastname: Mondal
mesh_terms: D000818:Animals; D000924:Anticholesteremic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002855:Chromatography, Thin Layer; D003445:Crustacea; D005285:Fermentation; D055951:Functional Food; D006868:Hydrolysis; D056692:Prebiotics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000924:Anticholesteremic Agents; D000975:Antioxidants; D056692:Prebiotics