title: Type-specific seroprevalence of bluetongue in Andhra Pradesh, India, during 2005-2009.
abstract: Bluetongue (BT) is an infectious, arthropod-borne viral disease of domestic and wild ruminants caused by bluetongue virus (BTV), which is a double-stranded segmented RNA virus. Of the 26 confirmed BTV serotypes, 23 were reported in India based on the detection of antibodies or virus. In order to assess the prevalence of different serotypes in Andhra Pradesh, serum samples which were positive for BTV by group-specific antibody ELISA were subjected to type-specific neutralization of BTV serotypes 1, 2, 9, 10, 21 and 23. Of the 52 samples tested, 50.0, 44.23, 21.15, 26.92, 0, and 15.38 % neutralized BTV serotypes 1, 2, 9, 10, 21 and 23, respectively. However, 32.69 % of the ELISA positive sera could not neutralize any of these serotypes, indicating that there could be other serotype viruses (e.g., BTV-3 and -16) circulating in the State. This method can be used for surveillance of the circulating serotypes as well as for assessing the level of herd immunity, and assist in determining the vaccine strains to be used in multivalent vaccines.
authors.affiliation: College of Veterinary Science, Sri Venkateswara Veterinary University, Hyderabad, 500030 India.
authors.forename: V
authors.initials: V
authors.lastname: Sairaju
authors.affiliation: College of Veterinary Science, Sri Venkateswara Veterinary University, Hyderabad, 500030 India.
authors.forename: B
authors.initials: B
authors.lastname: Susmitha
authors.affiliation: Ella Foundation, Genome Valley, Turkapally, Shameerpet Mandal, Hyderabad, 500078 India.
authors.forename: Pavuluri Panduranga
authors.initials: PP
authors.lastname: Rao
authors.affiliation: Ella Foundation, Genome Valley, Turkapally, Shameerpet Mandal, Hyderabad, 500078 India.
authors.forename: Nagendra R
authors.initials: NR
authors.lastname: Hegde
authors.affiliation: Ella Foundation, Genome Valley, Turkapally, Shameerpet Mandal, Hyderabad, 500078 India.
authors.forename: Keerti
authors.initials: K
authors.lastname: Meena
authors.affiliation: College of Veterinary Science, Sri Venkateswara Veterinary University, Hyderabad, 500030 India.
authors.forename: Y Narasimha
authors.initials: YN
authors.lastname: Reddy
keywords: Bluetongue virus; Epidemiology; Serotype; Surveillance; Virus neutralization assay