title: HIV-1 persistence in CD4+ T cells with stem cell-like properties.
abstract: Cellular HIV-1 reservoirs that persist despite antiretroviral treatment are incompletely defined. We show that during suppressive antiretroviral therapy, CD4(+) T memory stem cells (TSCM cells) harbor high per-cell levels of HIV-1 DNA and make increasing contributions to the total viral CD4(+) T cell reservoir over time. Moreover, we conducted phylogenetic studies that suggested long-term persistence of viral quasispecies in CD4(+) TSCM cells. Thus, HIV-1 may exploit the stem cell characteristics of cellular immune memory to promote long-term viral persistence.
authors.affiliation: 1] Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA. [2] Ragon Institute of MGH, MIT and Harvard, Cambridge, Massachusetts, USA.
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Buzon
authors.affiliation: 1] Ragon Institute of MGH, MIT and Harvard, Cambridge, Massachusetts, USA. [2] Key Laboratory of AIDS Immunology, Department of Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Sun
authors.affiliation: Ragon Institute of MGH, MIT and Harvard, Cambridge, Massachusetts, USA.
authors.forename: Chun
authors.initials: C
authors.lastname: Li
authors.affiliation: Ragon Institute of MGH, MIT and Harvard, Cambridge, Massachusetts, USA.
authors.forename: Amy
authors.initials: A
authors.lastname: Shaw
authors.affiliation: Ragon Institute of MGH, MIT and Harvard, Cambridge, Massachusetts, USA.
authors.forename: Katherine
authors.initials: K
authors.lastname: Seiss
authors.affiliation: Ragon Institute of MGH, MIT and Harvard, Cambridge, Massachusetts, USA.
authors.forename: Zhengyu
authors.initials: Z
authors.lastname: Ouyang
authors.affiliation: Ragon Institute of MGH, MIT and Harvard, Cambridge, Massachusetts, USA.
authors.forename: Enrique
authors.initials: E
authors.lastname: Martin-Gayo
authors.affiliation: Ragon Institute of MGH, MIT and Harvard, Cambridge, Massachusetts, USA.
authors.forename: Jin
authors.initials: J
authors.lastname: Leng
authors.affiliation: Division of Infectious Diseases, Brigham and Women's Hospital, Boston, Massachusetts, USA.
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Henrich
authors.affiliation: Division of Infectious Diseases, Brigham and Women's Hospital, Boston, Massachusetts, USA.
authors.forename: Jonathan Z
authors.initials: JZ
authors.lastname: Li
authors.affiliation: 1] Ragon Institute of MGH, MIT and Harvard, Cambridge, Massachusetts, USA. [2] Division of Infectious Diseases, Brigham and Women's Hospital, Boston, Massachusetts, USA.
authors.forename: Florencia
authors.initials: F
authors.lastname: Pereyra
authors.affiliation: Department of Electrical and Computer Engineering, University of Delaware, Newark, Delaware, USA.
authors.forename: Ryan
authors.initials: R
authors.lastname: Zurakowski
authors.affiliation: 1] Ragon Institute of MGH, MIT and Harvard, Cambridge, Massachusetts, USA. [2] Howard Hughes Medical Institute, Chevy Chase, Maryland, USA.
authors.forename: Bruce D
authors.initials: BD
authors.lastname: Walker
authors.affiliation: Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA.
authors.forename: Eric S
authors.initials: ES
authors.lastname: Rosenberg
authors.affiliation: Ragon Institute of MGH, MIT and Harvard, Cambridge, Massachusetts, USA.
authors.forename: Xu G
authors.initials: XG
authors.lastname: Yu
authors.affiliation: Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA.
authors.forename: Mathias
authors.initials: M
authors.lastname: Lichterfeld
mesh_terms: D023241:Antiretroviral Therapy, Highly Active; D001483:Base Sequence; D001900:Boston; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000821:virology*; D016000:Cluster Analysis; D004197:Disease Reservoirs / Q000821:virology*; D019143:Evolution, Molecular; D005434:Flow Cytometry; D015658:HIV Infections / Q000188:drug therapy / Q000821:virology*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D054503:Lymphoid Progenitor Cells / Q000166:cytology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D018709:Statistics, Nonparametric; D017735:Virus Latency; D054299:env Gene Products, Human Immunodeficiency Virus / Q000235:genetics
chemical_list: D054299:env Gene Products, Human Immunodeficiency Virus