title: Crambe tataria: the glucosinolate/myrosinase system in vivo and in vitro.
abstract: The Crambe tataria glucosinolate/myrosinase system in seeds and leaves of in vivo and in vitro regenerated plantlets, and two callus cell lines was investigated. It was demonstrated that in all the extracts glucosinolates were present and the myrosinase system was operative. There appears to be no discrimination between the glucosinolates used as substrates, but the hydrolysis rates were different regardless of the nature of the side chain. This is one of the first studies demonstrating that undifferentiated cells are able to synthetize glucosinolates and have an operating myrosinase system.
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, University of Padova, via Marzolo 5 - 35131 Padova, Italy. anna.piovan@unipd.it
authors.forename: Anna
authors.initials: A
authors.lastname: Piovan
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, University of Padova, via Marzolo 5 - 35131 Padova, Italy.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Filippini
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, University of Padova, via Marzolo 5 - 35131 Padova, Italy.
authors.forename: Rosy
authors.initials: R
authors.lastname: Caniato
mesh_terms: D002851:Chromatography, High Pressure Liquid; D031218:Crambe Plant / Q000378:metabolism*; D005961:Glucosinolates / Q000096:biosynthesis*; D006026:Glycoside Hydrolases / Q000502:physiology*
chemical_list: D005961:Glucosinolates; D006026:Glycoside Hydrolases; C022739:thioglucosidase