title: Biosynthesis of penicillin V acylase by Fusarium sp.: effect of culture conditions.
abstract: Penicillin V acylase was produced, both intracellularly and extracellularly, by Fusarium sp. SKF 235 grown in submerged fermentation. When neopeptone was added to the medium, >95% of the penicillin V acylase was extracellular. In the absence of a complex organic nitrogen source, the fungus produced low levels of totally intracellular penicillin V acylase. MgSO4 was essential for synthesis of the enzyme, which was induced by phenoxyacetic acid and penicillin V. The maximum yield of penicillin V acylase was 430 IU/g dry cell wt. The optimum pH value and temperature for the penicillin V acylase were 6.5 and 55°C, respectively.
authors.affiliation: Research and Development, Hindustan Antibiotics Ltd., 411 018, Pimpri, Pune, India.
authors.forename: V K
authors.initials: VK
authors.lastname: Sudhakaran
authors.forename: J G
authors.initials: JG
authors.lastname: Shewale