title: In vitro inhibitory effects of Moringa oleifera leaf extract and its major components on chemiluminescence and chemotactic activity of phagocytes.
abstract: The ethanol extract of Moringa oleifera Lam. leaves and its major constituents, crypto-chlorogenic acid, quercetin 3-O-glucoside and kaempferol 3-O-glucoside, were investigated on the respiratory burst of human whole blood and isolated human polymorphonuclear leukocytes (PMNs) using a luminol-based chemiluminescence assay. The chemotactic migration of PMNs was also investigated using the Boyden chamber technique. The ethanol extract demonstrated inhibitory activities on the oxidative burst and the chemotactic migration of PMNs. Quercetin 3-O-glucoside, crypto-chlorogenic acid, and kaempferol 3-O-glucoside, isolated from the extract, expressed relatively strong inhibitory activity on the oxidative burst of PMNs with IC50 values of 4.1, 6.7 and 7.0 microM, respectively, comparable with that of aspirin. They also demonstrated strong inhibition of chemotatic migration of PMNs with IC50 values of 9.5, 15.9 and 18.2 microM, respectively. The results suggest that M. oleifera leaves could modulate the immune response of human phagocytes, linking to its ethnopharmacological use as an anti-inflammatory agent. The immunomodulating activity of the plant was mainly due to its major components.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand.
authors.forename: Boonyadist
authors.initials: B
authors.lastname: Vongsak
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand.
authors.forename: Wandee
authors.initials: W
authors.lastname: Gritsanapan
authors.affiliation: Department of Physiology, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand.
authors.forename: Yuvadee
authors.initials: Y
authors.lastname: Wongkrajang
authors.affiliation: Drug and Herbal Research Center, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, Malaysia.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Jantan
mesh_terms: D002633:Chemotaxis / Q000187:drug effects*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D002726:Chlorogenic Acid / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology; D005960:Glucosides; D006801:Humans; D044949:Kaempferols / Q000494:pharmacology; D049449:Luminescence; D009005:Monosaccharides / Q000494:pharmacology; D029588:Moringa oleifera / Q000737:chemistry; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D010586:Phagocytes / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D011794:Quercetin / Q000031:analogs & derivatives; D016897:Respiratory Burst / Q000187:drug effects
chemical_list: D005419:Flavonoids; D005960:Glucosides; D044949:Kaempferols; D009005:Monosaccharides; D010936:Plant Extracts; C511963:kaempferol-3-O-glucoside; C532085:quercetin 3'-O-glucoside; D002726:Chlorogenic Acid; D011794:Quercetin