title: Planobispora takensis sp. nov., isolated from soil.
abstract: A novel Gram-stain-positive, filamentous bacterial strain (A-T 7458(T)) was isolated from soil collected from hill evergreen forest in Thailand. The strain developed cylindrical sporangia containing a longitudinal pair of motile spores, on short ramifications of aerial mycelia. From a taxonomic study using a polyphasic approach, strain A-T 7458(T) had typical characteristics of members of the genus Planobispora. 16S rRNA gene sequence analysis indicated that the isolate was closely related to Planobispora siamensis A-T 4600(T) (98.5 %), Planobispora rosea JCM 3166(T) (97.6 %) and Planobispora longispora NBRC 13918(T) (97.6 %). The DNA-DNA relatedness values, which differentiated the novel strain from the closest species, were significantly below 70 %. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained ribose, madurose, mannose and glucose. The predominant menaquinone was MK-9(H2). The diagnostic phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylinositol-mannoside and aminophosphoglycolipid. The predominant cellular fatty acids were unsaturated C17 : 1 and C18 : 1, and saturated C16 : 0 and C17 : 0. Following an evaluation of phenotypic, chemotaxonomic and genotypic characteristics, the novel isolate is proposed to represent a novel species of the genus Planobispora, to be named Planobispora takensis sp. nov. The type strain is A-T 7458(T) ( = BCC 48396(T) = NBRC 109077(T)).
authors.affiliation: BIOTEC Culture Collection, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phaholyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand.
authors.forename: Wipaporn
authors.initials: W
authors.lastname: Ngaemthao
authors.affiliation: BIOTEC Culture Collection, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phaholyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand.
authors.forename: Chanwit
authors.initials: C
authors.lastname: Suriyachadkun
authors.affiliation: BIOTEC Culture Collection, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phaholyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand.
authors.forename: Suwanee
authors.initials: S
authors.lastname: Chunhametha
authors.affiliation: Department of Biology and Actinobacterial Research Unit, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok, Thailand.
authors.forename: Nantawan
authors.initials: N
authors.lastname: Niemhom
authors.affiliation: Department of Biology and Actinobacterial Research Unit, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok, Thailand.
authors.forename: Chitti
authors.initials: C
authors.lastname: Thawai
authors.affiliation: Novartis Institutes for BioMedical Research Basel, Natural Products Unit, Novartis Pharma AG, Basel, Switzerland.|BIOTEC Culture Collection, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phaholyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand.
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Sanglier
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013785:Thailand; D014197:Trees / Q000382:microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9; D003960:Diaminopimelic Acid