title: [Primary culture and morphological characteristics of human lacrimal adenoid cystic carcinoma cells].
abstract: OBJECTIVE
  To explore a method of primary culturing human adenoid cystic carcinoma cells of lacrimal gland.
  
  
  METHODS
  Experiment research. Tumor tissue was obtained from the surgical material of a patient diagnosed as lacrimal adenoid cystic carcinoma in Tianjin Medical University Eye Hospital during May 16th to June 1st.We gained primary cells via tissue culture techniques. Mixed cells were removed through several ways.Observed cell morphological characteristics by phase contrast microscope, scanning electron microscope and transmission electron microscope. Cyto-immunochemical staining was applied to detect the expressions of vimentin, desmin, S-100, cytokeratin, pan and CD34. Their expressions were also detected in the tumor tissue except CD34. Made cell growth curve and calculated cell doubling time.
  
  
  RESULTS
  The outgrowth of cells was observed by day 5 after seeding tissues, and then cells generated slowly. The first passage proceeded by day 32, and the classical epithelioid cell colonies was observed by day 69 after inoculation. Purified cells of human lacrimal adenoid cystic carcinoma were obtained after the removal of mixed cells through several ways, which have been successfully subcultured for more than 100 passages. The 25th passage LACC cells appeared to be typically epithelioid cells, they showed contact inhibition as the density high enough.SEM and TEM showed the 25th passage LACC cells were malignant tumor cells poorly differentiated. They showed positive reaction with vimentin, cytokeratin (pan) as well as S-100, but negative reaction with desmin and CD34, which were consistent with the tumor tissue. The cell growth curve turned like a sigmoid one, and the cell doubling time was 37.1 h.
  
  
  CONCLUSIONS
  We gained purified LACC cells, and understood the morphological characteristics, laying the foundation for the establishment of a human lacrimal adenoid cystic carcinoma cell line.
authors.affiliation: Tianjin Medical University Eye Hospital, Tianjin 300384, China.
authors.forename: Bei-qing
authors.initials: BQ
authors.lastname: Zhou
authors.affiliation: Tianjin Medical University Eye Hospital, Tianjin 300384, China. Email: yanjinhe88@gmail.com.
authors.forename: Yan-jin
authors.initials: YJ
authors.lastname: He
authors.affiliation: Tianjin Medical University Eye Hospital, Tianjin 300384, China.
authors.forename: Ting-ting
authors.initials: TT
authors.lastname: Lin
authors.affiliation: Tianjin Medical University Eye Hospital, Tianjin 300384, China.
authors.forename: Li-min
authors.initials: LM
authors.lastname: Zhu
mesh_terms: D003528:Carcinoma, Adenoid Cystic / Q000473:pathology*; D018929:Cell Culture Techniques; D005134:Eye Neoplasms / Q000473:pathology*; D006801:Humans; D007766:Lacrimal Apparatus Diseases / Q000473:pathology*; D014407:Tumor Cells, Cultured