title: Synthetic biology in mammalian cells: next generation research tools and therapeutics.
abstract: Recent progress in DNA manipulation and gene circuit engineering has greatly improved our ability to programme and probe mammalian cell behaviour. These advances have led to a new generation of synthetic biology research tools and potential therapeutic applications. Programmable DNA-binding domains and RNA regulators are leading to unprecedented control of gene expression and elucidation of gene function. Rebuilding complex biological circuits such as T cell receptor signalling in isolation from their natural context has deepened our understanding of network motifs and signalling pathways. Synthetic biology is also leading to innovative therapeutic interventions based on cell-based therapies, protein drugs, vaccines and gene therapies.
authors.affiliation: Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Florian
authors.initials: F
authors.lastname: Lienert
authors.affiliation: 1] Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115, USA. [2] Department of Immunology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA.
authors.forename: Jason J
authors.initials: JJ
authors.lastname: Lohmueller
authors.affiliation: Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Garg
authors.affiliation: Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Pamela A
authors.initials: PA
authors.lastname: Silver
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D053263:Gene Regulatory Networks / Q000235:genetics; D015316:Genetic Therapy; D006801:Humans; D008322:Mammals; D058990:Molecular Targeted Therapy; D012313:RNA / Q000737:chemistry / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics*; D015398:Signal Transduction; D058615:Synthetic Biology
chemical_list: D004268:DNA-Binding Proteins; D016601:RNA-Binding Proteins; D012313:RNA; D004247:DNA