title: The analysis of carbohydrates in milk powder by a new "heart-cutting" two-dimensional liquid chromatography method.
abstract: In this study, a new"heart-cutting" two-dimensional liquid chromatography method for the simultaneous determination of carbohydrate contents in milk powder was presented. In this two dimensional liquid chromatography system, a Venusil XBP-C4 analysis column was used in the first dimension ((1)D) as a pre-separation column, a ZORBAX carbohydrates analysis column was used in the second dimension ((2)D) as a final-analysis column. The whole process was completed in less than 35min without a particular sample preparation procedure. The capability of the new two dimensional HPLC method was demonstrated in the determination of carbohydrates in various brands of milk powder samples. A conventional one dimensional chromatography method was also proposed. The two proposed methods were both validated in terms of linearity, limits of detection, accuracy and precision. The comparison between the results obtained with the two methods showed that the new and completely automated two dimensional liquid chromatography method is more suitable for milk powder sample because of its online cleanup effect involved.
authors.affiliation: School of Medicine, Xi'an Jiaotong University, 76#, Yanta West Road, Xi'an 710061, Shaanxi, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ma
authors.affiliation: School of Medicine, Xi'an Jiaotong University, 76#, Yanta West Road, Xi'an 710061, Shaanxi, China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Hou
authors.affiliation: School of Medicine, Xi'an Jiaotong University, 76#, Yanta West Road, Xi'an 710061, Shaanxi, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
authors.affiliation: School of Medicine, Xi'an Jiaotong University, 76#, Yanta West Road, Xi'an 710061, Shaanxi, China.
authors.forename: Yunan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Medicine, Xi'an Jiaotong University, 76#, Yanta West Road, Xi'an 710061, Shaanxi, China. Electronic address: helc@mail.xjtu.edu.cn.
authors.forename: Langchong
authors.initials: L
authors.lastname: He
mesh_terms: D000818:Animals; D002241:Carbohydrates / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008892:Milk / Q000737:chemistry*; D011208:Powders / Q000737:chemistry*
chemical_list: D002241:Carbohydrates; D011208:Powders
keywords: Carbohydrates; Milk powder; Refractive index detector; Two-dimensional liquid chromatography