title: Effects of surfactants on cell growth and pigment production in suspension cultures ofPerilla frutescens.
abstract: The effects of surfactants, adecanol LG-294 and silicone A, on anthocyanin accumulation and the growth ofPerilla frutescens cells in suspension cultures were studied. Production of the red pigment was remarkably reduced from about 1.9 g/l to 0.4 g/l by adecanol LG-294 at 0.06 ml/l but not by silicone A up to 0.4 ml/l. Several repeated shake-flask cultures also demonstrated no adverse effects of silicone A on the metabolite accumulation by the suspended cells. Furthermore, the addition of silicone A to a culture in a stirred bioreactor produced a three-fold higher growth rate and a seven-fold increase in anthocyanin compared with surfactant-free cultures. The improvement was due to the substantial reduction or prevention of foaming and of cell adhesion to the bioreactor wall.
authors.affiliation: International Center of Cooperative Research in Biotechnology, Faculty of Engineering, Osaka University, 2-1 Yamada-oka, 565, Suita-shi, Osaka, Japan.
authors.forename: J J
authors.initials: JJ
authors.lastname: Zhong
authors.forename: T
authors.initials: T
authors.lastname: Seki
authors.forename: S I
authors.initials: SI
authors.lastname: Kinoshita
authors.forename: T
authors.initials: T
authors.lastname: Yoshida