title: Antiviral activity and underlying molecular mechanisms of Matrine against porcine reproductive and respiratory syndrome virus in vitro.
abstract: Porcine reproductive and respiratory syndrome (PRRS), caused by porcine reproductive and respiratory syndrome virus (PRRSV), is an acute infectious disease. The prevalence of PRRS has made swine industry suffered huge financial losses. Matrine, a natural compound, has been demonstrated to possess anti-PRRSV activity in Marc-145 cells. However, the underlying molecular mechanisms were still unknown. The main objective of our study was to discuss the effect of Matrine on PRRSV N protein expression and PRRSV induced apoptosis. Indirect immunofluorescence assay (IFA) and Western blot were used to assess the effect of Matrine on N protein expression. Apoptosis was analyzed by fluorescence staining. In addition, the effect of Matrine on caspase-3 activation was investigated by Western blot. Indirect immunofluorescence assay and Western blot analysis demonstrated that Matrine could inhibit N protein expression in Marc-145 cells. And Matrine was found to be able to impair PRRSV-induced apoptosis by inhibiting caspase-3 activation.
authors.affiliation: College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, PR China.
authors.forename: Na
authors.initials: N
authors.lastname: Sun
authors.affiliation: College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, PR China.
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Wang
authors.affiliation: Jiangsu Animal Husbandry & Veterinary College, Taizhou, Jiangsu 225300, PR China.
authors.forename: Cai-Hong
authors.initials: CH
authors.lastname: Wu
authors.affiliation: College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, PR China.
authors.forename: E
authors.initials: E
authors.lastname: Li
authors.affiliation: College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, PR China.
authors.forename: Jun-Ping
authors.initials: JP
authors.lastname: He
authors.affiliation: University of Western Sydney, School of Medicine, Locked Bag 1797, Penrith 2751, NSW, Australia.
authors.forename: Shao-Yu
authors.initials: SY
authors.lastname: Wang
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Yuan-Liang
authors.initials: YL
authors.lastname: Hu
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, PR China.
authors.forename: Hai-Min
authors.initials: HM
authors.lastname: Lei
authors.affiliation: College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, PR China. Electronic address: livets@163.com.
authors.forename: Hong-Quan
authors.initials: HQ
authors.lastname: Li
mesh_terms: D000470:Alkaloids / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000276:immunology*; D015153:Blotting, Western / Q000662:veterinary; D002460:Cell Line; D002522:Chlorocebus aethiops; D008856:Microscopy, Fluorescence / Q000662:veterinary; D019590:Nucleocapsid Proteins / Q000276:immunology; D019318:Porcine Reproductive and Respiratory Syndrome / Q000188:drug therapy* / Q000276:immunology; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D011807:Quinolizines / Q000494:pharmacology* / Q000627:therapeutic use; D013552:Swine; D000093842:Matrines
chemical_list: D000470:Alkaloids; D019590:Nucleocapsid Proteins; D011807:Quinolizines; D000093842:Matrines
keywords: Antiviral; Apoptosis; Matrine; N protein; PRRSV