title: Synthesis, biophysical studies and RNA interference activity of RNA having three consecutive amide linkages.
abstract: RNA sequences having up to three consecutive internal amide linkages were synthesized and studied using UV and NMR spectroscopy. The amide modifications did not interfere with normal base-pairing and A-type RNA conformation. Three consecutive amides were well tolerated in the passenger strand of siRNA and caused little change in RNAi activity.
authors.affiliation: Department of Chemistry, Binghamton University, The State University of New York, Binghamton, New York 13902, USA. erozners@binghamton.edu.
authors.forename: Paul
authors.initials: P
authors.lastname: Tanui
authors.forename: Scott D
authors.initials: SD
authors.lastname: Kennedy
authors.forename: Benjamin D
authors.initials: BD
authors.lastname: Lunstad
authors.forename: Amanda
authors.initials: A
authors.lastname: Haas
authors.forename: Devin
authors.initials: D
authors.lastname: Leake
authors.forename: Eriks
authors.initials: E
authors.lastname: Rozners
mesh_terms: D000577:Amides / Q000737:chemistry*; D001483:Base Sequence; D006367:HeLa Cells; D006801:Humans; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000138:chemical synthesis / Q000737:chemistry* / Q000235:genetics*
chemical_list: D000577:Amides; D034741:RNA, Small Interfering