title: Characterization of a photosystem II reaction center complex isolated by exposure of PSII membranes to a non-ionic detergent and high concentrations of NaCl.
abstract: A highly resolved PSII reaction center complex has been prepared by exposure of PSII membranes to the detergent octylglucopyranoside at elevated ionic strengths; oxygen evolution activity is about 1,000 μmoles O2/hr/mg Chl in the presence of CaCl2. A Mn quantitation and a kinetic study of Z, the donor to P680, reveals that on a Chl basis this new preparation shows an almost four-fold enrichment in Mn and the electron transport components of PSII.
authors.affiliation: Division of Biological Sciences, The University of Michigan, 48109-1048, Ann Arbor, MI, USA.
authors.forename: D F
authors.initials: DF
authors.lastname: Ghanotakis
authors.forename: C F
authors.initials: CF
authors.lastname: Yocum