title: Bio-hybrid silk fibroin/calcium phosphate/PLGA nanocomposite scaffold to control the delivery of vascular endothelial growth factor.
abstract: This study investigated the efficacy of bio-hybrid silk fibroin/Calcium phosphate/PLGA nanocomposite scaffold as vascular endothelial growth factor (VEGF) delivery system. The scaffold was fabricated using freeze-drying and electrospinning. Here, we highlight the structural changes of the scaffold using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy and differential scanning calorimetry (DSC). The uniform dispersion of calcium phosohate (CaP) powder within silk fibroin (SF) solution was also confirmed using Zeta potential analysis. Moreover, good biocompatibility of osteoblast cells next to the scaffold was approved by cell adhesion, proliferation and alkaline phosphatase production. The release profile of VEGF during 28 days has established the efficacy of the scaffold as a sustained delivery system. The bioactivity of the released VEGF was maintained about 83%. The histology analysis has shown that the new bone tissue formation happened in the defected site after 10 weeks of implantation. Generally, our data showed that the fabricated scaffold could be considered as an effective scaffold for bone tissue engineering applications.
authors.affiliation: Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: mehdi13294@yahoo.com.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Farokhi
authors.affiliation: Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University (TMU), Tehran, Iran. Electronic address: fatemeh.motaghi@gmail.com.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Mottaghitalab
authors.affiliation: National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran. Electronic address: mashokrgozar@pasteur.ac.ir.
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Shokrgozar
authors.affiliation: Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: jafar_ai@tums.ac.ir.
authors.forename: Jafar
authors.initials: J
authors.lastname: Ai
authors.affiliation: Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Jamshid
authors.initials: J
authors.lastname: Hadjati
authors.affiliation: Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Azami
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis; D002130:Calcium Phosphates / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003460:Crystallization / Q000379:methods; D004058:Diffusion; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005349:Fibroins / Q000737:chemistry*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D008422:Materials Testing; D053769:Nanocapsules / Q000008:administration & dosage* / Q000737:chemistry; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013499:Surface Properties; D023822:Tissue Engineering / Q000295:instrumentation; D054457:Tissue Scaffolds; D042461:Vascular Endothelial Growth Factor A / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D053769:Nanocapsules; D042461:Vascular Endothelial Growth Factor A; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D005349:Fibroins; C020243:calcium phosphate
keywords: Calcium phosphate; Nanocomposite; PLGA; Silk fibroin; Sustained delivery system; VEGF