title: Cell concentration as a factor in precursor incorporation by tobacco leaf protoplasts or separated cells.
abstract: Freshly isolated protoplasts or separated cells prepared from leaves of Nicotiana tabacum L. cvs. Xanthincor Turkish Samsun exhibit substantially greater incorporation of [(3)H] leucine and [(3)H] uridine into protoplast protein and RNA, respectively, when dilute rather than concentrated suspensions are incubated. This phenomenon is considered to result from a dilution of the labeled precursors in the incubation medium by nonlabeled precursors of cellular origin.
authors.affiliation: Department of Plant Pathology, Cornell University, 14853, Ithaca, NY, USA.
authors.forename: G
authors.initials: G
authors.lastname: Rubin
authors.forename: M
authors.initials: M
authors.lastname: Zaitlin