title: Effects of weekly blood collection in male and female cynomolgus macaques (Macaca fascicularis).
abstract: This study was designed to evaluate the maximal amount of blood that can be safely collected in healthy, adult male and female cynomolgus macaques for 4 consecutive weeks with minimal effect on animal wellbeing. General guidelines for blood collection volumes in laboratory animals are not species-specific, and currently there are few evaluations of blood collection in macaques. In this study, blood was removed at 7.5%, 10%, 12.5%, 15%, or 17.5% of total blood volume (TBV) for 4 consecutive weeks. Hematologic parameters and body weights were evaluated immediately prior to each blood collection time point and for an additional 4 consecutive weeks following the last collection. Male and female macaques tolerated removal of as much as 15% TBV with minor clinical effects, whereas macaques in the 17.5% TBV group exhibited an increased incidence of emesis and anorexia during the first 24 h after blood collection. According to these results, we recommend collecting no more than 15% TBV weekly for 4 consecutive weeks from healthy, adult male and female cynomolgus macaques.
authors.affiliation: Biologic Resources Laboratory, University of Illinois at Chicago, Chicago, Illinois, USA. cloboda@uic.edu.
authors.forename: Cynthia R
authors.initials: CR
authors.lastname: Adams
authors.affiliation: Biologic Resources Laboratory, University of Illinois at Chicago, Chicago, Illinois, USA.
authors.forename: Lisa C
authors.initials: LC
authors.lastname: Halliday
authors.affiliation: Biologic Resources Laboratory, University of Illinois at Chicago, Chicago, Illinois, USA.
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Nunamaker
authors.affiliation: Biologic Resources Laboratory, University of Illinois at Chicago, Chicago, Illinois, USA.
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Fortman
mesh_terms: D000818:Animals; D000830:Animals, Laboratory; D001800:Blood Specimen Collection / Q000009:adverse effects / Q000379:methods; D001810:Blood Volume; D005260:Female; D008252:Macaca fascicularis; D008297:Male; D013997:Time Factors