title: Ferriphaselus amnicola gen. nov., sp. nov., a neutrophilic, stalk-forming, iron-oxidizing bacterium isolated from an iron-rich groundwater seep.
abstract: A neutrophilic, stalk-forming, iron-oxidizing bacterium, strain OYT1(T), which was isolated from a groundwater seep in Ohyato Park, Tokyo, Japan, was subjected to taxonomic analysis. OYT1(T) was a motile, bean-shaped, Gram-negative bacterium that was able to grow at 8-30 °C (optimally at 25-30 °C) and at pH 5.6-7.3 (optimally at pH 6.1-6.5). The strain grew microaerobically and autotrophically. Major cellular fatty acids detected were C16 : 1ω7c/C16 : 1ω6c and C16 : 0. The total DNA G+C content was 57.6 mol%. 16S rRNA gene sequence analysis revealed that strain OYT1(T) was affiliated with the class Betaproteobacteria and clustered with iron-oxidizing bacteria isolated from groundwater seeps and wetlands and with uncultured clones detected in freshwater iron-rich environments. Based on the phenotypic and phylogenetic characteristics of strain OYT1(T), we propose that the strain represents a novel species in a new genus, for which the name Ferriphaselus amnicola gen. nov., sp. nov. is proposed; the type strain of Ferriphaselus amnicola is OYT1(T) ( = JCM 18545(T) = DSM 26810(T)).
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba, Ibaraki, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Kato
authors.affiliation: Department of Geological Sciences, University of Delaware, Newark, DE, USA.
authors.forename: Sean
authors.initials: S
authors.lastname: Krepski
authors.affiliation: Department of Geological Sciences, University of Delaware, Newark, DE, USA.
authors.forename: Clara
authors.initials: C
authors.lastname: Chan
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba, Ibaraki, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Itoh
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba, Ibaraki, Japan.
authors.forename: Moriya
authors.initials: M
authors.lastname: Ohkuma
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D043372:Gallionellaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D060587:Groundwater / Q000382:microbiology*; D007501:Iron / Q000378:metabolism*; D016013:Likelihood Functions; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014041:Tokyo
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D007501:Iron