title: Experimental susceptibility of Caspian white fish, Rutilus frisii kutum to Spring viraemia of carp virus.
abstract: Caspian white fish (Rutilus frisii kutum) is a fish of the family Cyprinidae, which is commercially harvested from the Caspian Sea. Experimental infection with Spring viraemia of carp virus (SVCV) was conducted in order to examine susceptibility of caspian White Fish and clinical impacts of infection. Fingerling fish were injected intra-peritoneally or waterborne-exposed with SVCV and were monitored daily for 7 weeks. Dead fish and those survived at the end of experimental period were collected for virus isolation and reverse transcriptase polymerase chain reaction analysis. Epithelioma papulosum cyprini cell line was used to re-isolate the virus and indirect fluorescent antibody test was conducted to identify the isolated virus. Infection trials showed that SVCV was highly pathogenic for the Caspian White Fish with mortality rate ranging from 75 to 85 %, depending on the viral challenge model. SVCV genome was detected from dead and apparently healthy fish tissues of both virus exposure models, which showed Caspian White Fish not only can be regarded as a susceptible host, but also serve as a vector of the virus.
authors.affiliation: Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Evin, Tehran, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Zamani
authors.affiliation: Department of Fish Diseases, Inland Water Aquaculture Institute, Iranian Fisheries Research Organization, P.O. Box 66, Anzali, Gilan Iran.
authors.forename: M
authors.initials: M
authors.lastname: Ghasemi
authors.affiliation: Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Evin, Tehran, Iran.
authors.forename: S M
authors.initials: SM
authors.lastname: Hosseini
authors.affiliation: Department of Fisheries, Anzali Branch, Islamic Azad University, Anzali, Gilan Iran.
authors.forename: S
authors.initials: S
authors.lastname: Haghighi Karsidani
keywords: EPC; IFAT; Inoculation; TCID50