title: Aberrant histone methylation in the patients with immune thrombocytopenia.
abstract: Abstract The aim of the present study was to investigate alterations in histone methylation in patients with primary immune thrombocytopenia (ITP). Global histone H3K4/H3K9 methylation in CD4+ T cells from 35 ITP patients and 15 healthy controls were measured using the EpiQuik(TM) global histone H3K4/H3K9 methylation assay kits. The mRNA expression of SUV39H1, SUV39H2 and EZH2 were detected by real-time quantitative polymerase chain reaction (RT-PCR). The results showed that global histone H3K9 hypomethylation in CD4+ T cells of active ITP, compared with ITP in remission and controls, while the global histone H3K4 methylation were not significantly different between ITP patients and healthy controls. The expression of EZH2 and SUV39H2 were significantly down-regulated in active ITP patients, when compared with ITP in remission and controls. There were not different between ITP patients and controls in the expression SUV39H1. In conclusion, the aberrant histone methylation was involved in the pathogenesis of ITP.
authors.affiliation: Department of Hematology and Oncology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy , Tianjin , PR China .
authors.forename: Haifeng
authors.initials: H
authors.lastname: Zhao
authors.forename: Feng
authors.initials: F
authors.lastname: Xue
authors.forename: Jianfen
authors.initials: J
authors.lastname: Xu
authors.forename: Zhi
authors.initials: Z
authors.lastname: Fang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015496:CD4-Positive T-Lymphocytes / Q000201:enzymology / Q000378:metabolism; D016022:Case-Control Studies; D002648:Child; D015536:Down-Regulation; D000071221:Enhancer of Zeste Homolog 2 Protein; D005260:Female; D000076983:Histone Methyltransferases; D011495:Histone-Lysine N-Methyltransferase / Q000096:biosynthesis / Q000097:blood; D006657:Histones / Q000097:blood*; D006801:Humans; D008297:Male; D008745:Methylation; D008875:Middle Aged; D063151:Polycomb Repressive Complex 2 / Q000097:blood / Q000378:metabolism; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000378:metabolism; D055815:Young Adult
chemical_list: D006657:Histones; D000076983:Histone Methyltransferases; C498409:EZH2 protein, human; D000071221:Enhancer of Zeste Homolog 2 Protein; D011495:Histone-Lysine N-Methyltransferase; D063151:Polycomb Repressive Complex 2