title: Direct, immunological and molecular techniques for a fasciolosis survey in a rural area of San Luis, Argentina.
abstract: Fasciolosis is a zoonosis caused by the trematode Fasciola hepatica, prevalent in cattle, that is actually emerging as a cause of disease in humans. The goal of this work was to describe the characteristics of fasciolosis in arroyo El Juncal region, La Toma, San Luis province, Argentina. In order to get this objective, a transversal, quantitative study was carried out by a fieldwork that allowed the collection of data, human, animal, and environmental samples. The materials were processed by direct, immunological and/or molecular diagnostic techniques. According to the geographical characteristics and in presence of all the definitive and intermediate hosts, reservoirs, and sources of infection, it was possible to describe the persistence of fasciolosis in the area. The prevalence was 11.90 % in humans (by serology), 5.26 % in cattle (by coprological analysis) and 61.76 % in snails (by PCR). The situation that was found for this area indicates that any measure of intervention for the control of this zoonosis should be adopted by multidisciplinary teams.
authors.affiliation: Instituto Nacional de Enfermedades Infecciosas, ANLIS "Dr. Carlos G. Malbrán", Avenida Vélez Sársfield 563, CP 1281 Buenos Aires, Argentina ; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Avenida Rivadavia 1917, CP 1033 Buenos Aires, Argentina.
authors.forename: Silvana
authors.initials: S
authors.lastname: Carnevale
authors.affiliation: Instituto Nacional de Enfermedades Infecciosas, ANLIS "Dr. Carlos G. Malbrán", Avenida Vélez Sársfield 563, CP 1281 Buenos Aires, Argentina.
authors.forename: Marta Graciela
authors.initials: MG
authors.lastname: Cabrera
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Avenida Rivadavia 1917, CP 1033 Buenos Aires, Argentina.
authors.forename: Marcela Alejandra
authors.initials: MA
authors.lastname: Cucher
authors.affiliation: Hospital Municipal General de Agudos "Dr. José María Penna", Pedro Chutro 3380, CP 1437 Buenos Aires, Argentina.
authors.forename: Cecilia Alicia
authors.initials: CA
authors.lastname: di Risio
authors.affiliation: Facultad de Ciencias de la Salud, Universidad Nacional de Catamarca, Maestro Quiroga 1ra. Cuadra, CP 4700 San Fernando del Valle de Catamarca, Argentina.
authors.forename: Jorge Bruno
authors.initials: JB
authors.lastname: Malandrini
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Avenida Rivadavia 1917, CP 1033 Buenos Aires, Argentina.
authors.forename: Laura
authors.initials: L
authors.lastname: Kamenetzky
authors.affiliation: Maestría en Epidemiología, Gestión y Políticas de Salud, Universidad Nacional de Lanús, Avenida 29 de Septiembre 3901 edificio "Leonardo Werthein", Remedios de Escalada, CP 1826 Lanús, Buenos Aires Argentina.
authors.forename: Marcio
authors.initials: M
authors.lastname: Alazraqui
authors.affiliation: Hospital Municipal General de Agudos "Dr. José María Penna", Pedro Chutro 3380, CP 1437 Buenos Aires, Argentina.
authors.forename: Cristina Beatriz
authors.initials: CB
authors.lastname: Etchart
authors.affiliation: Instituto Nacional de Enfermedades Infecciosas, ANLIS "Dr. Carlos G. Malbrán", Avenida Vélez Sársfield 563, CP 1281 Buenos Aires, Argentina.
authors.forename: María Laura
authors.initials: ML
authors.lastname: Pantano
authors.affiliation: Hospital Municipal de Infecciosas "Dr. Francisco Javier Muñiz", Uspallata 2272, CP 1282, Buenos Aires, Argentina.
authors.forename: Jorge Néstor
authors.initials: JN
authors.lastname: Velásquez
keywords: Epidemiology; Fasciola hepatica; Fasciolosis