title: Distribution of glyoxylate-cycle enzymes between microbodies and mitochondria in Aspergillus tamarii.
abstract: The distribution of glyoxylate-cycle enzymes between microbodies and mitochondria was examined in ethanol-grown Aspergillus tamarii Kita. Particulate activities of catalase and the two glyoxylate by-pass enzymes, malate synthase and isocitrate lyase, were localized in the microbodies. The microbodies had a buoyant density of about 1.23 g cm(-3) after isopycnic centrifugation in linear sucrose gradients. Particulate activities of the other two glyoxycitrate synthase, together with that of succinate dehydrogenase were restricted to the mitochondria, which had a buoyant density of about 1.20 g cm(-3). Catalase also appeared to be localized in a second particle, perhaps the microbody inclusions or the Woronin bodies, having a buoyant density of about 1.26 g cm(-3).
authors.affiliation: Department of Plant Pathology, University of Wisconsin, 53706, Madison, WI, USA.
authors.forename: L B
authors.initials: LB
authors.lastname: Graves
authors.forename: V N
authors.initials: VN
authors.lastname: Armentrout
authors.forename: D P
authors.initials: DP
authors.lastname: Maxwell