title: Pre-earthquake non-epidemic Vibrio cholerae in Haiti.
abstract: BACKGROUND
  To our knowledge, there was no record of Vibrio cholerae in Haiti until the 2010 post earthquake outbreak.
  
  
  METHODS
  This study describes the analysis of 301 stool samples from 117 infants in Port-au-Prince, Haiti, who participated in a pediatric nutrition study between July 2008 and October 2009.
  
  
  RESULTS
  Nine samples were identified positive with both SYBR Green and Taqman-MGB probe based molecular assays targeting V. cholerae hlyA and toxR, respectively (Ct = 33-40), but none were O1 or O139.
  
  
  CONCLUSIONS
  Our results from multiple molecular assays demonstrate the presence of non-O1/O139 V. cholerae DNA in stools collected from nine asymptomatic Haitian infants two years prior to the 2010 earthquake.
authors.affiliation: University of Virginia, Charlottesville, VA, USA. jl5yj@virginia.edu.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Christopher
authors.initials: C
authors.lastname: Winstead-Derlega
authors.forename: Eric
authors.initials: E
authors.lastname: Houpt
authors.forename: Rebecca
authors.initials: R
authors.lastname: Heidkamp
authors.forename: Jean
authors.initials: J
authors.lastname: Pape
authors.forename: Rebecca
authors.initials: R
authors.lastname: Dillingham
mesh_terms: D002771:Cholera / Q000453:epidemiology* / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D055866:Earthquakes; D005243:Feces / Q000382:microbiology; D005260:Female; D006205:Haiti / Q000453:epidemiology; D006801:Humans; D007223:Infant; D008297:Male; D015345:Oligonucleotide Probes / Q000235:genetics; D044163:Vibrio cholerae non-O1 / Q000145:classification / Q000302:isolation & purification*; D037521:Virulence Factors / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D015345:Oligonucleotide Probes; D037521:Virulence Factors