title: [Expression of KLF-8 and MMP-9 in placentas and their relationship with the pathogenesis of preeclampsia].
abstract: OBJECTIVE
  To evaluate the expression of Krüppel-like factor 8 (KLF-8) and matrix metalloproteinase 9 (MMP-9) in placentas and their relationship with the pathogenesis of preeclampsia (PE).
  
  
  METHODS
  Twenty-two women with PE(mild PE:4 cases; severe PE:18 cases) who received cesarean sections in the First Affiliated Hospital of Chongqing Medical University from September 2011 to March 2012 were recruited as the PE group (n = 22). And twenty women who received elective term cesarean section without perinatal complications were chosen as the control group (n = 20). Placentas were collected and immunohistochemical SP method were employed to detect the localization of KLF-8 protein.KLF-8 mRNA level was determined by quantitative real-time PCR technique and western blot analysis was used to quantify KLF-8 and MMP-9 protein levels.
  
  
  RESULTS
  (1) There was no difference of KLF-8 protein distribution in placentas of the PE group and the control group.It was mainly located in the nuclear and cytoplasm of syncytiotrophoblasts.KLF-8 immunostaining was apparently decreased in the placentas of preeclamptic women when compared with the control group.(2)The KLF-8 mRNA levels were significantly decreased in placentas of the PE group (0.69 ± 0.08) compared to those of the control group (1.14 ± 0.09, P < 0.01). (3) KLF-8 and MMP-9 protein levels significantly decreased in the PE placentas (0.68 ± 0.05 and 0.21 ± 0.03) when compared to the control group (0.94 ± 0.06 and 0.34 ± 0.03, respectively, P < 0.01).(4) There was a positive correlation between the expression of KLF-8 and MMP-9 protein in the placentas from PE and normal pregnancies (r = 0.64, P < 0.01).
  
  
  CONCLUSIONS
  KLF-8 mRNA and protein levels were decreased in placentas of PE patients compared to those of normotensive women.KLF-8 protein was primarily located in the invasion-related trophoblast cells and its expression had a positive correlation with MMP-9 levels.KLF-8 might have an important role in the pathogenesis of PE by regulation of trophoblast invasion.
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Zhong-mei
authors.initials: ZM
authors.lastname: Yang
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Luo
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Bai
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China. Email:qihongbo@sina.com.
authors.forename: Hong-bo
authors.initials: HB
authors.lastname: Qi
mesh_terms: D000328:Adult; D015153:Blotting, Western; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014327:Trophoblasts / Q000378:metabolism
chemical_list: C507402:KLF8 protein, human; D051741:Kruppel-Like Transcription Factors; D012333:RNA, Messenger; D012097:Repressor Proteins; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9