title: Melanin from the nitrogen-fixing bacterium Azotobacter chroococcum: a spectroscopic characterization.
abstract: Melanins, the ubiquitous hetero-polymer pigments found widely dispersed among various life forms, are usually dark brown/black in colour. Although melanins have variety of biological functions, including protection against ultraviolet radiation of sunlight and are used in medicine, cosmetics, extraction of melanin from the animal and plant kingdoms is not an easy task. Using complementary physicochemical techniques (i.e. MALDI-TOF, FTIR absorption and cross-polarization magic angle spinning solid-state (13)C NMR), we report here the characterization of melanins extracted from the nitrogen-fixing non-virulent bacterium Azotobacter chroococcum, a safe viable source. Moreover, considering dihydroxyindole moiety as the main constituent, an effort is made to propose the putative molecular structure of the melanin hetero-polymer extracted from the bacterium. Characterization of the melanin obtained from Azotobacter chroococcum would provide an inspiration in extending research activities on these hetero-polymers and their use as protective agent against UV radiation.
authors.affiliation: Department of Bioinformatics, West Bengal University of Technology, Salt Lake, Kolkata, W.B., India.
authors.forename: Aulie
authors.initials: A
authors.lastname: Banerjee
authors.affiliation: Department of Bioinformatics, West Bengal University of Technology, Salt Lake, Kolkata, W.B., India.
authors.forename: Subhrangshu
authors.initials: S
authors.lastname: Supakar
authors.affiliation: Department of Bioinformatics, West Bengal University of Technology, Salt Lake, Kolkata, W.B., India.
authors.forename: Raja
authors.initials: R
authors.lastname: Banerjee
mesh_terms: D001395:Azotobacter / Q000737:chemistry* / Q000378:metabolism*; D008543:Melanins / Q000737:chemistry* / Q000302:isolation & purification; D009586:Nitrogen Fixation; D013057:Spectrum Analysis
chemical_list: D008543:Melanins