title: [Effect of over-expressed monocarboxylate transporter on the biological behavior of MDA-MB-231 breast cancer cells].
abstract: OBJECTIVE
  To investigate the effect of over-expressed monocarboxylate transporter (MCT) on apoptosis, cell cycle, migration and invasion of MDA-MB-231 breast cancer cells.
  
  
  METHODS
  Eukaryotic expression plasmid pcDNA3.1/MCT and empty plasmid pcDNA3.1 were respectively transfected into MDA-MB-231 cells. The expression of MCT gene was tested by real-time quantitative PCR (qRT-PCR) and Western blotting. After stained respectively by annexin V-FITC/PI and PI, the apoptosis and cell cycle in the MDA-MB-231 cells over-expressing MCT were detected by flow cytometry. The abilities of invasion and migration in the MDA-MB-231 cells with over-expressed MCT were respectively determined by Transwell(TM); and scratch wound healing assays.
  
  
  RESULTS
  In mRNA and protein levels, the expression of MCT was higher in pcDNA3.1/MCT transfected group than in negative control group and untreated group. Over-expression of MCT in the MDA-MB-231 cells enhanced cell apoptosis, reduced the cell number of G2 phase and increased the cell number of S phase. The abilities of invasion and migration in the MDA-MB-231 cells were inhibited significantly.
  
  
  CONCLUSIONS
  Over-expression of MCT in the MDA-MB-231 cells promotes apoptosis, regulates G2/M checkpoint and inhibits the abilities of invasion and migration.
authors.affiliation: Molecular Medicine and Cancer Research Center, Department of Biochemistry and Molecular Biology, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Ma
authors.affiliation: Molecular Medicine and Cancer Research Center, Department of Biochemistry and Molecular Biology, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Li
authors.initials: L
authors.lastname: Mai
authors.affiliation: Molecular Medicine and Cancer Research Center, Department of Biochemistry and Molecular Biology, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Wu
authors.affiliation: Molecular Medicine and Cancer Research Center; Center of Molecular Tumor, Department of Biochemistry and Molecular Biology, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Changdong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Molecular Medicine and Cancer Research Center, Department of Biochemistry and Molecular Biology, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Yu
authors.affiliation: Molecular Medicine and Cancer Research Center, Department of Biochemistry and Molecular Biology, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Haiyu
authors.initials: H
authors.lastname: Li
authors.affiliation: Molecular Medicine and Cancer Research Center, Department of Biochemistry and Molecular Biology, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.affiliation: Molecular Medicine and Cancer Research Center, Department of Biochemistry and Molecular Biology, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Molecular Medicine and Cancer Research Center; Center of Molecular Tumor, Department of Biochemistry and Molecular Biology, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Fangzhou
authors.initials: F
authors.lastname: Song
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001943:Breast Neoplasms / Q000473:pathology*; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D027501:Monocarboxylic Acid Transporters / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness; D014162:Transfection
chemical_list: D027501:Monocarboxylic Acid Transporters