title: Anti-inflammatory effects of mannanase-hydrolyzed copra meal in a porcine model of colitis.
abstract: We evaluated the anti-inflammatory activity of mannanase-hydrolyzed copra meal (MNB), including β-1,4-mannobiose (67.8%), in a dextran sodium sulfate (DSS)-induced porcine model of intestinal inflammation. In the DSS-positive control (POS) and MNB treatment (MCM) groups, DSS was first administered to piglets via intragastric catheter for 5 days, followed by 5 days administration of saline or MCM. A negative control group (NEG) received a saline alternative to DSS and MNB. Inflammation was assessed by clinical signs, morphological and histological measurements, gut permeability and neutrophil infiltration. Local production of TNF-α and IL-6 were analyzed by ELISA, colonic and ileal inflammatory gene expressions were assessed by real time RT-PCR, and CD4+CD25+ cell populations were analyzed by flow cytometry. Crypt elongation and muscle thickness, D-mannitol gut permeation, colonic expression of the inflammatory mediators TNF-α and IL-6 and myeloperoxidase activity were significantly lower in the MCM group than in that of POS group. The mRNA levels of ileal IL-1β, IL-6, IL-17 and TNF-α were significantly lower following MCM treatment than with POS treatment.MNB exerts anti-inflammatory activity in vivo, suggesting that MNB is a novel therapeutic that may provide relief to human and animals suffering from intestinal inflammation.
authors.affiliation: R&D Institute, Fuji Oil Co., Ltd., Izumisano-shi, Osaka 598-8540, Japan.
authors.forename: Masahisa
authors.initials: M
authors.lastname: Ibuki
authors.forename: Kensuke
authors.initials: K
authors.lastname: Fukui
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kanatani
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Mine
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D003059:Cocos / Q000378:metabolism*; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology; D016264:Dextran Sulfate / Q000009:adverse effects; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005434:Flow Cytometry / Q000662:veterinary; D006868:Hydrolysis; D015850:Interleukin-6 / Q000378:metabolism; D008361:Mannosidases / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016264:Dextran Sulfate; D009195:Peroxidase; D008361:Mannosidases