title: A cascading reaction sequence involving ligand-directed azaelectrocyclization and autooxidation-induced fluorescence recovery enables visualization of target proteins on the surfaces of live cells.
abstract: A general probe designed to induce a cascading sequence of reactions on a target protein was efficiently synthesized. The cascading reaction sequence involved (i) ligand-directed azaelectrocyclization with lysine and (ii) the autooxidation-induced release of a fluorescence quencher from the labeled protein. The probe was linked to a cyclic RGDyK peptide to enable the selective visualization of integrin αVβ3 on the surfaces of live cells.
authors.affiliation: Biofunctional Synthetic Chemistry Laboratory, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan. kotzenori@riken.jp.
authors.forename: Katsunori
authors.initials: K
authors.lastname: Tanaka
authors.forename: Masataka
authors.initials: M
authors.lastname: Kitadani
authors.forename: Ayumi
authors.initials: A
authors.lastname: Tsutsui
authors.forename: Ambara R
authors.initials: AR
authors.lastname: Pradipta
authors.forename: Rie
authors.initials: R
authors.lastname: Imamaki
authors.forename: Shinobu
authors.initials: S
authors.lastname: Kitazume
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Taniguchi
authors.forename: Koichi
authors.initials: K
authors.lastname: Fukase
mesh_terms: D000818:Animals; D001372:Aza Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival; D003500:Cyclization; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D008024:Ligands; D051379:Mice; D008958:Models, Molecular; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010456:Peptides, Cyclic / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001372:Aza Compounds; D005456:Fluorescent Dyes; D008024:Ligands; D010456:Peptides, Cyclic; D011506:Proteins