title: Diversity Analysis of Bacterial Community from Permafrost Soil of Mo-he in China.
abstract: The permafrost soil of Mo-he in Northeast China presents a typical cold environment colonized by psychrophilic microorganisms. This study is aimed at assessing the bacterial communities of permafrost soil of Mo-he in China by sequencing the 16S rRNA genes and Mothur analysis. PCR products with universal 16S rRNA gene primers were cloned and partially sequenced, and bacterial identification at the species was performed by comparative analysis with the GenBank/EMBL/DDBJ database. A total of 266 clones were obtained with the average length of 1,050 bp. Mothur analysis showed that the coverage value of clone library was 53.78 %, Shannon diversity (H) was 4.03, Simpson diversity value was 0.018, and 74 operational taxonomic units were generated. Through phylogenetic assignment using BLASTN by more than 97 % similarity, a total of 87 tentative taxa were identified. The majority of bacterial sequences recovered in this study belonged to the Acidobacteria, Proteobacteria, Verrucomicrobia, Bacteroidetes, Chloroflexi and Chlorobi. Among them, Acidobacteria are dominant community, accounting for 30.1 % of total bacteria, followed by Proteobacteria which accounted for 22.2 %. This result reflected the acidic characteristics of the permafrost soil of which pH value was 6.0. Our study indicated that the permafrost soil of Mo-he in China has a high diversity of bacteria and represents a vast potential resource of novel bacteria. As far as we knew, this is the first report on bacterial diversity of permafrost soil of Mo-he in China.
authors.affiliation: Biocontrol Microbes Lab, Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, No. 9 Shuguang Huayuan Mid Road, Haidian District, Beijing, 100097 China.
authors.forename: Dong
authors.initials: D
authors.lastname: Dan
authors.affiliation: Biocontrol Microbes Lab, Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, No. 9 Shuguang Huayuan Mid Road, Haidian District, Beijing, 100097 China.
authors.forename: Dian-Peng
authors.initials: DP
authors.lastname: Zhang
authors.affiliation: Biocontrol Microbes Lab, Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, No. 9 Shuguang Huayuan Mid Road, Haidian District, Beijing, 100097 China.
authors.forename: Wei-Cheng
authors.initials: WC
authors.lastname: Liu
authors.affiliation: Biocontrol Microbes Lab, Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, No. 9 Shuguang Huayuan Mid Road, Haidian District, Beijing, 100097 China.
authors.forename: Cai-Ge
authors.initials: CG
authors.lastname: Lu
authors.affiliation: Biocontrol Microbes Lab, Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, No. 9 Shuguang Huayuan Mid Road, Haidian District, Beijing, 100097 China.
authors.forename: Tao-Tao
authors.initials: TT
authors.lastname: Zhang
keywords: Bacterial diversity; Mo-he; Permafrost soil