title: Rhodoferax saidenbachensis sp. nov., a psychrotolerant, very slowly growing bacterium within the family Comamonadaceae, proposal of appropriate taxonomic position of Albidiferax ferrireducens strain T118T in the genus Rhodoferax and emended description of the genus Rhodoferax.
abstract: A Gram-stain-negative, oxidase and phosphatase-positive and catalase-negative, short rod-shaped bacterium was isolated from sediment of a drinking water reservoir in Germany. Based on 16S rRNA gene sequence and phenotypic properties, the bacterium belongs to the genus Rhodoferax within the family Comamonadaceae. The new taxon differed from related species mainly with respect to its fatty acid composition, low growth temperature, lack of pigments in young cultures and ability to utilize glycerol and d-mannose but not urea. The major fatty acids were C16 : 1ω7c and/or iso-C15 : 0 2-OH, C16 : 0, and C18 : 1ω7c. The only ubiquinone detected was ubiquinone Q-8. The DNA G+C content was 60.3-61 mol%. Because of the phenotypic and genotypic differences from the most closely related taxa, the new strain represents a novel species for which the name Rhodoferax saidenbachensis sp. nov. is proposed. The type strain is ED16(T) ( = CCUG 57711(T) = ATCC BAA-1852(T) = DSM 22694(T)). An emended description of the genus Rhodoferax is proposed. Based on the results of this study, strain T118(T) (Albidiferax ferrireducens) is properly placed in the genus Rhodoferax as Rhodoferax ferrireducens.
authors.affiliation: National Veterinary Institute SVA, Ulls väg 2b, 75189 Uppsala, Sweden.
authors.forename: René
authors.initials: R
authors.lastname: Kaden
authors.affiliation: Leibnitz Institute DSMZ, Inhoffenstr. 7b, 38124 Braunschweig, Germany.
authors.forename: Cathrin
authors.initials: C
authors.lastname: Spröer
authors.affiliation: UFZ Centre for Environmental Research, Permoserstr. 15, 04318 Leipzig, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Beyer
authors.affiliation: Karlsruhe Institute of Technology, Institute of Functional Interfaces Division, PO Box 3640, D-76021 Karlsruhe, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Krolla-Sidenstein
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D003080:Cold Temperature; D042621:Comamonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D060766:Drinking Water / Q000382:microbiology*; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D019015:Geologic Sediments / Q000382:microbiology; D005858:Germany; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry; D014881:Water Supply
chemical_list: D004269:DNA, Bacterial; D060766:Drinking Water; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8