title: hnRNP A2/B1 interacts with influenza A viral protein NS1 and inhibits virus replication potentially through suppressing NS1 RNA/protein levels and NS1 mRNA nuclear export.
abstract: The NS1 protein of influenza viruses is a major virulence factor and exerts its function through interacting with viral/cellular RNAs and proteins. In this study, we identified heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1) as an interacting partner of NS1 proteins by a proteomic method. Knockdown of hnRNP A2/B1 by small interfering RNA (siRNA) resulted in higher levels of NS vRNA, NS1 mRNA, and NS1 protein in the virus-infected cells. In addition, we demonstrated that hnRNP A2/B1 proteins are associated with NS1 and NS2 mRNAs and that knockdown of hnRNP A2/B1 promotes transport of NS1 mRNA from the nucleus to the cytoplasm in the infected cells. Lastly, we showed that knockdown of hnRNP A2/B1 leads to enhanced virus replication. Our results suggest that hnRNP A2/B1 plays an inhibitory role in the replication of influenza A virus in host cells potentially through suppressing NS1 RNA/protein levels and NS1 mRNA nucleocytoplasmic translocation.
authors.affiliation: Department of Biological Sciences, University of Arkansas, Fayetteville, Arkansas, United States.
authors.forename: Yimeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Biological Sciences, University of Arkansas, Fayetteville, Arkansas, United States.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Biological Sciences, University of Arkansas, Fayetteville, Arkansas, United States.
authors.forename: Yuchun
authors.initials: Y
authors.lastname: Du
mesh_terms: D021581:Active Transport, Cell Nucleus; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism / Q000821:virology; D015536:Down-Regulation; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000378:metabolism*; D007251:Influenza, Human / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; C085820:INS1 protein, influenza virus; D012333:RNA, Messenger; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins; C439768:hnRNP A2
keywords: Influenza A virus; NS1; Protein–protein interaction; Proteomics; hnRNP A2/B1; mRNA nuclear export