title: Chromatin-bound DNA polymerase from higher plants : A DNA polymerase-β-like enzyme.
abstract: Chromatin-bound DNA polymerase has been extracted from pea (Pisum sativum L.) seedlings, and partially purified by solubilization from chromatin followed by chromatography on columns of either DEAE-cellulose or DEAE-Sephadex. The enzyme elutes from DEAE-cellulose as a single peak, but is fractionated into two peaks, CI and CII, by DEAE-Sephadex chromatography. If the enzyme is stored at-15°C for several days prior to chromatography, a third peak, CIII, derived from CII, is obtained. The polymerase is devoid of nuclease activity, and is relatively insensitive to N-ethyl-maleimide. These features, taken with the ion requirements and with data obtained from other plant species, lead to the suggestion that the chromatin-bound DNA polymerase of higher plants is similar to the DNA polymerase-β from vertebrates.
authors.affiliation: Department of Botany, University College, P.O. Box 78, CF1 1XL, Cardiff, UK.
authors.forename: C
authors.initials: C
authors.lastname: Stevens
authors.forename: J A
authors.initials: JA
authors.lastname: Bryant
authors.forename: P C
authors.initials: PC
authors.lastname: Wyvill