title: Pulse-chase analysis to measure protein degradation.
abstract: In order to measure protein half-life by pulse-chase analysis, cells are incubated with labeled precursors, [(35)S]-methionine and [(35)S]-cysteine, for a short time (pulse), after which they are washed from the labeled compound and incubated with excess cold precursor (chase). The decay of the amount of protein labeled during the pulse period is measured during the chase with cold precursor.
authors.affiliation: Faculty of Medicine, Technion - I.I.T. and Rappaport Institute for Biomedical Research, Haifa, Israel.
authors.forename: Einav
authors.initials: E
authors.lastname: Simon
authors.affiliation: Faculty of Medicine, Technion - I.I.T. and Rappaport Institute for Biomedical Research, Haifa, Israel. Electronic address: danielk@tx.technion.ac.il.
authors.forename: Daniel
authors.initials: D
authors.lastname: Kornitzer
mesh_terms: D002176:Candida albicans / Q000378:metabolism; D006207:Half-Life; D007553:Isotope Labeling; D008715:Methionine / Q000378:metabolism; D059748:Proteolysis; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D008715:Methionine
keywords: Cellular proteins; Cytoplasmic pools; Derivation of protein half-life; Immunoprecipitation; Methionine pulse-chase; Protein degradation; Pulse-chase analysis