title: Glycyrrhizin attenuates tissue injury and reduces neutrophil accumulation in experimental acute pancreatitis.
abstract: Leukocyte infiltration and acinar cell injury are characteristic features of acute pancreatitis (AP). However, the signaling pathways regulating inflammation and accumulation of leukocytes into pancreas tissue remains poorly elucidated. In the current study, we investigated the effects of Glycyrrhizin (GZ) on cerulein-induced AP in mice. AP was induced in male C57BL/6 by intraperitoneal injection of 50 μg/kg cerulein hourly, with a total of 7 times. 1 hour after the last injection of cerulean, mice were treated with either 35 or 70 mg/kg of GZ. Serum amylases and lipases were measured using automated chromogenic assay, MCP-1 and MIP-2 concentrations were measured in the serum by ELISA, and the number of infiltrated inflammatory cells in the pancreas were evaluated by flow cytometry. We found that GZ treatment resulted in reduction (i) both amylase and lipase activities, (ii) the serum levels of both MCP-1 and MIP-2; and (iii) markedly attenuated cerulein-induced histopathological alternations and water contents. Furthermore, we observed that GZ significantly decreased the number of infiltrated monocytes and neutrophils into the pancreas tissue. In conclusion, we demonstrate that GZ attenuates AP signs and inhibits inflammatory cell recruitments into pancreas.
authors.affiliation: Kurdistan Cellular & Molecular Research Center, Kurdistan University of Medical Sciences Sanandaj, Iran.
authors.forename: Shohreh
authors.initials: S
authors.lastname: Fakhari
authors.affiliation: Department of Immunology & Hematology, Faculty of Medicine, Kurdistan University of Medical Sciences Sanandaj, Iran.
authors.forename: Kamal
authors.initials: K
authors.lastname: Abdolmohammadi
authors.affiliation: Department of Immunology & Hematology, Faculty of Medicine, Kurdistan University of Medical Sciences Sanandaj, Iran.
authors.forename: Yaser
authors.initials: Y
authors.lastname: Panahi
authors.affiliation: Kurdistan Cellular & Molecular Research Center, Kurdistan University of Medical Sciences Sanandaj, Iran.
authors.forename: Bahram
authors.initials: B
authors.lastname: Nikkhoo
authors.affiliation: Kurdistan Cellular & Molecular Research Center, Kurdistan University of Medical Sciences Sanandaj, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Peirmohammadi
authors.affiliation: Department of Immunology & Hematology, Faculty of Medicine, Kurdistan University of Medical Sciences Sanandaj, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Rahmani
authors.affiliation: Immunology Research Center, Iran University of Medical Sciences Tehran, Iran.
authors.forename: Alireza Salek
authors.initials: AS
authors.lastname: Moghadam
authors.affiliation: Kurdistan Cellular & Molecular Research Center, Kurdistan University of Medical Sciences Sanandaj, Iran ; Department of Immunology & Hematology, Faculty of Medicine, Kurdistan University of Medical Sciences Sanandaj, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Jalili
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D019695:Glycyrrhizic Acid / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020556:Neutrophil Infiltration / Q000187:drug effects*; D010195:Pancreatitis / Q000276:immunology* / Q000473:pathology
chemical_list: D000893:Anti-Inflammatory Agents; D019695:Glycyrrhizic Acid
keywords: Glycyrrhizin; experimental acute pancreatitis; leukocyte infiltration; neutrophil accumulation; tissue injury