title: Cluster of differentiation antibody microarrays on plasma immersion ion implanted polycarbonate.
abstract: Plasma immersion ion implantation (PIII) modifies the surface properties of polymers, enabling them to covalently immobilize proteins without using linker chemistry. We describe the use of PIII treated polycarbonate (PC) slides as a novel platform for producing microarrays of cluster of differentiation (CD) antibodies. We compare their performance to identical antibody microarrays printed on nitrocellulose-coated glass slides that are currently the industry standard. Populations of leukocytes are applied to the CD microarrays and unbound cells are removed revealing patterns of differentially immobilized cells that are detected in a simple label-free approach by scanning the slides with visible light. Intra-slide and inter-slide reproducibility, densities of bound cells, and limits of detection were determined. Compared to the nitrocellulose-coated glass slides, PIII treated PC slides have a lower background noise, better sensitivity, and comparable or better reproducibility. They require three-fold lower antibody concentrations to yield equivalent signal strength, resulting in significant reductions in production cost. The improved transparency of PIII treated PC in the near-UV and visible wavelengths combined with superior immobilization of biomolecules makes them an attractive platform for a wide range of microarray applications.
authors.affiliation: Department of Applied Plasma and Physics, School of Physics, University of Sydney, Sydney, 2006, Australia. Electronic address: elenak@physics.usyd.edu.au.
authors.forename: E
authors.initials: E
authors.lastname: Kosobrodova
authors.affiliation: Muscle Research Unit, Discipline of Anatomy and Histology, Bosch Institute, University of Sydney, Sydney, 2006, Australia.
authors.forename: A
authors.initials: A
authors.lastname: Mohamed
authors.affiliation: Australian Center of Microscopy and Analysis, University of Sydney, Sydney, 2006, Australia.
authors.forename: Y
authors.initials: Y
authors.lastname: Su
authors.affiliation: Department of Applied Plasma and Physics, School of Physics, University of Sydney, Sydney, 2006, Australia.
authors.forename: A
authors.initials: A
authors.lastname: Kondyurin
authors.affiliation: Muscle Research Unit, Discipline of Anatomy and Histology, Bosch Institute, University of Sydney, Sydney, 2006, Australia.
authors.forename: C G
authors.initials: CG
authors.lastname: dos Remedios
authors.affiliation: Department of Applied Plasma and Physics, School of Physics, University of Sydney, Sydney, 2006, Australia.
authors.forename: D R
authors.initials: DR
authors.lastname: McKenzie
authors.affiliation: Department of Applied Plasma and Physics, School of Physics, University of Sydney, Sydney, 2006, Australia.
authors.forename: M M M
authors.initials: MM
authors.lastname: Bilek
mesh_terms: D015703:Antigens, CD / Q000032:analysis / Q000276:immunology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D007477:Ions; D058626:Plasma Gases / Q000737:chemistry*; D011076:Polycarboxylate Cement / Q000737:chemistry* / Q000528:radiation effects; D040081:Protein Array Analysis / Q000295:instrumentation*; D012031:Refractometry / Q000295:instrumentation*; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation
chemical_list: D015703:Antigens, CD; D007477:Ions; D058626:Plasma Gases; D011076:Polycarboxylate Cement; C005506:polycarbonate
keywords: Antibody microarray; Cluster of differentiation antibody; Label-free proteomics; Plasma immersion ion implantation; Polycarbonate