title: Systematic nucleo-cytoplasmic trafficking of proteins following exposure of MCF7 breast cancer cells to estradiol.
abstract: We have used a proteomics subcellular spatial razor approach to look at changes in total protein abundance and in protein distribution between the nucleus and cytoplasm following exposure of MCF7 breast cancer cells to estradiol. The dominant response of MCF7 cells to estrogen stimulation involves dynamic changes in protein subcellular spatial distribution rather than changes in total protein abundance. Of the 3604 quantitatively monitored proteins, only about 2% show substantial changes in total abundance (>2-fold), whereas about 20% of the proteins show substantial changes in local abundance and/or redistribution of their subcellular location, with up to 16-fold changes in their local concentration in the nucleus or the cytoplasm. We propose that dynamic redistribution of the subcellular location of multiple proteins in response to stimuli is a fundamental characteristic of cells and suggest that perturbation of cellular spatial control may be an important feature of cancer.
authors.affiliation: Proteomics and Molecular Cell Dynamics, Center for Nephrology, Division of Medicine, School of Life and Medical Sciences, University College London , Royal Free Campus, Rowland Hill Street, London NW3 2PF, United Kingdom.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Pinto
authors.forename: Abdulrab Ahmed M
authors.initials: AA
authors.lastname: Alhaiek
authors.forename: Sepan
authors.initials: S
authors.lastname: Amadi
authors.forename: Amal T
authors.initials: AT
authors.lastname: Qattan
authors.forename: Mark
authors.initials: M
authors.lastname: Crawford
authors.forename: Marko
authors.initials: M
authors.lastname: Radulovic
authors.forename: Jasminka
authors.initials: J
authors.lastname: Godovac-Zimmermann
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000187:drug effects*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D018613:Microscopy, Confocal; D009363:Neoplasm Proteins / Q000378:metabolism*
chemical_list: D009363:Neoplasm Proteins; D004958:Estradiol