title: [Study on enzyme extraction technology of total flavonoids from Cryptotaenia japonica].
abstract: OBJECTIVE
  To study the optimum enzyme extraction process of total flavonoids from Cryptotaenia japonica.
  
  
  METHODS
  The process was optimized by single factor experiments and orthogonal, the yield of total flavonoids was used as index.
  
  
  RESULTS
  The optimum extraction technology was as follows: enzyme dosage: 1U/mL, pH value: 6.0, enzymatic hydrolysis temperature: 60%, enzymatic hydrolysis time: 1 h, the rate of material to liquid: 1 : 30, extraction temperature: 70 degrees C, extraction time: 1 h, the average extraction rate of total flavonoids from Cryptotaenia japonica was 4.76%.
  
  
  CONCLUSIONS
  The method is an effective way to extract total flavonoids from Cryptotaenia japonica with high extract rate.
authors.affiliation: College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China. 690056167@qq.com
authors.forename: Jun
authors.initials: J
authors.lastname: Lu
authors.affiliation: College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China.
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Xu
authors.affiliation: College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China.
authors.forename: Ou
authors.initials: O
authors.lastname: Rong
authors.affiliation: College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China.
authors.forename: Shang-Bin
authors.initials: SB
authors.lastname: Li
authors.affiliation: College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China.
authors.forename: Chi
authors.initials: C
authors.lastname: Zhou
authors.affiliation: College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China.
authors.forename: Zhong-Hai
authors.initials: ZH
authors.lastname: Li
mesh_terms: D019661:Apiaceae / Q000737:chemistry*; D002480:Cellulase / Q000378:metabolism*; D005419:Flavonoids / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
chemical_list: D005419:Flavonoids; D012997:Solvents; D002480:Cellulase