title: Seasonal changes in expression of nerve growth factor and its receptors TrkA and p75 in the ovary of wild ground squirrel (Citellus dauricus Brandt).
abstract: The aim of this study was to investigate the presence of nerve growth factor (NGF) and its receptors tyrosine kinase A (TrkA) and p75 in the ovaries of the wild ground squirrels during the breeding and nonbreeding seasons. In the breeding period, NGF, TrkA and p75 were immunolocalized in granulosa cells, thecal cells, interstitial cells and luteal cells whereas in the nonbreeding period, both of them were detected only in granulosa cells, thecal cells and interstitial cells. Stronger immunostaining of NGF, TrkA and p75 were observed in granulosa cells, thecal cells and interstitial cells in the breeding season compared to the nonbreeding season. Corresponding for the immunohistochemical results, immunoreactivities of NGF and its two receptors were greater in the ovaries of the breeding season then decreased to a relatively low level in the nonbreeding season. The mean mRNA levels of NGF, TrkA and p75 were significantly higher in the breeding season than in the nonbreeding season. In addition, plasma gonadotropins, estradiol-17β and progesterone concentrations were significantly higher in the breeding season than in the nonbreeding season, suggesting that the expression patterns of NGF, and TrkA and p75 were correlated with changes in plasma gonadotropins, estradiol-17β and progesterone concentrations. These results indicated that NGF and its receptors, TrkA and p75 may be involved in the regulation of seasonal changes in the ovarian functions of the wild ground squirrel.
authors.forename: Ben
authors.initials: B
authors.lastname: Li
authors.forename: Xia
authors.initials: X
authors.lastname: Sheng
authors.forename: Lihong
authors.initials: L
authors.lastname: Bao
authors.forename: Shiyang
authors.initials: S
authors.lastname: Huang
authors.forename: Qinglin
authors.initials: Q
authors.lastname: Li
authors.forename: Yuning
authors.initials: Y
authors.lastname: Liu
authors.forename: Yingying
authors.initials: Y
authors.lastname: Han
authors.forename: Gen
authors.initials: G
authors.lastname: Watanabe
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Taya
authors.affiliation: Laboratory of Animal Physiology, College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China. qiangweng@bjfu.edu.cn.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Weng
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D004958:Estradiol / Q000097:blood; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006062:Gonadotropins / Q000097:blood; D020932:Nerve Growth Factor / Q000235:genetics / Q000378:metabolism*; D010053:Ovary / Q000166:cytology / Q000201:enzymology*; D011374:Progesterone / Q000097:blood; D012333:RNA, Messenger / Q000378:metabolism; D020800:Receptor, Nerve Growth Factor / Q000235:genetics / Q000378:metabolism*; D020917:Receptor, trkA / Q000235:genetics / Q000378:metabolism*; D012098:Reproduction; D012589:Sciuridae / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D012621:Seasons; D013997:Time Factors
chemical_list: D015415:Biomarkers; D006062:Gonadotropins; D012333:RNA, Messenger; D020800:Receptor, Nerve Growth Factor; D011374:Progesterone; D004958:Estradiol; D020932:Nerve Growth Factor; D020917:Receptor, trkA