title: Mucosal and systemic immunity in mice after intranasal immunization with recombinant Lactococcus lactis expressing ORF6 of PRRSV.
abstract: The purpose of the study was to construct mucosal vaccine of a recombinant Lactococcus lactis expressing PRRSV ORF6 gene and evaluate mucosal and systemic immune response against PRRSV in mice after intranasal immunization. The result show that the vaccine can stimulate mice to produce specific IgG in serum and remarkable special s-IgA in lung lavage fluid, at the same time, the contents of cytokines IL-2 and IFN-γ of the experimental group were significant higher than those of the control group (P < 0.01), however, the contents of cytokines IL-4 was not different to the all groups. In summary, the constructed mucosal vaccine can significantly induce mucosal immune, humoral immunity and cellular immunity involved Th1 type cytokines, which will lay a theoretical foundation on immune mechanism and new efficient vaccines for PRRSV.
authors.affiliation: Department of Animal and Veterinary Science, Chengdu Vocational College of Agricultural Science and Technology, WenJiang, Sichuan 611130, PR China.
authors.forename: Zhen-hua
authors.initials: ZH
authors.lastname: Wang
authors.affiliation: Isotope Research Laboratory, College of Life and Basic Sciences, Sichuan Agricultural University, Xin Kang Road 46, Ya'an, Sichuan 625014, PR China.
authors.forename: Xiao-han
authors.initials: XH
authors.lastname: Cao
authors.affiliation: Isotope Research Laboratory, College of Life and Basic Sciences, Sichuan Agricultural University, Xin Kang Road 46, Ya'an, Sichuan 625014, PR China.
authors.forename: Xiao-gang
authors.initials: XG
authors.lastname: Du
authors.affiliation: Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, PR China.
authors.forename: Hai-bo
authors.initials: HB
authors.lastname: Feng
authors.affiliation: Department of Animal and Veterinary Science, Chengdu Vocational College of Agricultural Science and Technology, WenJiang, Sichuan 611130, PR China.
authors.lastname: Di-Wang
authors.affiliation: Department of Animal and Veterinary Science, Chengdu Vocational College of Agricultural Science and Technology, WenJiang, Sichuan 611130, PR China.
authors.lastname: He-Song
authors.affiliation: Isotope Research Laboratory, College of Life and Basic Sciences, Sichuan Agricultural University, Xin Kang Road 46, Ya'an, Sichuan 625014, PR China. Electronic address: 363150834@qq.com.
authors.forename: Xian-yin
authors.initials: XY
authors.lastname: Zeng
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology; D005260:Female; D005818:Genetic Engineering; D005822:Genetic Vectors; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D013294:Lactococcus lactis; D051379:Mice; D008807:Mice, Inbred BALB C; D009297:Nasal Mucosa / Q000276:immunology*; D009561:Nisin / Q000378:metabolism; D016366:Open Reading Frames / Q000235:genetics / Q000276:immunology; D019318:Porcine Reproductive and Respiratory Syndrome / Q000517:prevention & control*; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D013552:Swine; D018417:Th1 Cells / Q000276:immunology; D058408:Th1-Th2 Balance; D014611:Vaccination / Q000379:methods; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology*
chemical_list: D016207:Cytokines; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D014764:Viral Proteins; D014765:Viral Vaccines; D009561:Nisin
keywords: Intranasal immunization; Lactococcus lactis; Mucosal immunity; PRRSV-ORF6