title: Partial purification and characterization of the soluble DNA polymerase (polymerase-α) from seedlings of Pisum sativum L.
abstract: Soluble DNA polymerase has been extracted from pea seedlings and partially purified by chromatography on columns of DEAE-cellulose or DEAE-Sephadex. The enzyme elutes from DEAE-cellulose as a single peak, but is fractionated into three peaks, SI, SIa and SII by DEAE-Sephadex chromatography. SIa and SII may be derived from SI by freeze-thaw treatment or by treatment with (NH4)2SO4. The ion and pH requirements and the sensitivity to N-ethyl maleimide of the pea seedling soluble DNA polymerase are similar to those of the DNA polymerase-α from vertebrates.
authors.affiliation: Department of Botany, University College, P.O. Box 78, CF1 1XL, Cardiff, UK.
authors.forename: C
authors.initials: C
authors.lastname: Stevens
authors.forename: J A
authors.initials: JA
authors.lastname: Bryant