title: A mouse bone marrow stromal cell line with skeletal stem cell characteristics to study osteogenesis in vitro and in vivo.
abstract: Bone marrow stromal cells (BMSCs) are composed of progenitor and multipotent skeletal stem cells, which are able to differentiate in vitro into osteocytes, adipocytes, and chondrocytes. Mouse BMSCs (mBMSCs) are a versatile model system to investigate factors involved in BMSC differentiation in vitro and in vivo as a variety of transgenic mouse models are available. In this study, mBMSCs were isolated and osteogenic differentiation was investigated in tissue culture and in vivo. Three out of seven independent cell isolates showed the ability to differentiate into osteocytes, adipocytes, and chondrocytes in vitro. In vitro multipotency of an established mBMSC line was maintained over 45 passages. The osteogenic differentiation of this cell line was confirmed by quantitative polymerase chain reaction (qPCR) analysis of specific markers such as osteocalcin and shown to be Runx2 dependent. Notably, the cell line, when transplanted subcutaneously into mice, possesses full skeletal stem cell characteristics in vivo in early and late passages, evident from bone tissue formation, induction of vascularization, and hematopoiesis. This cell line provides, thus, a versatile tool to unravel the molecular mechanisms governing osteogenesis in vivo thereby aiding to improve current strategies in bone regenerative therapy.
authors.affiliation: 1 Institute of Cell Biology and Immunology, University of Stuttgart , Stuttgart, Germany .
authors.forename: Sebastian
authors.initials: S
authors.lastname: Raeth
authors.forename: Benedetto
authors.initials: B
authors.lastname: Sacchetti
authors.forename: Georg
authors.initials: G
authors.lastname: Siegel
authors.forename: Ulrike A
authors.initials: UA
authors.lastname: Mau-Holzmann
authors.forename: Jan
authors.initials: J
authors.lastname: Hansmann
authors.forename: Gabriele
authors.initials: G
authors.lastname: Vacun
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Hauk
authors.forename: Klaus
authors.initials: K
authors.lastname: Pfizenmaier
authors.forename: Angelika
authors.initials: A
authors.lastname: Hausser
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D051379:Mice; D039902:Multipotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D010012:Osteogenesis; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; C493716:Runx2 protein, mouse