title: [Expression of PARP/NF-κB and intervention effect of 5-AIQ/PDTC in SAP rats with adrenal damage].
abstract: OBJECTIVE
  To explore the expression of poly (ADP-ribose) polymerase/nuclear factor-κB (PARP/NF-κB) and intervention effect of 5-aminoisoquinolinone/pyrrolidine dithiocarbamate (5-AIQ/PDTC) in severe acute pancreatitis (SAP) rats with adrenal damage.
  
  
  METHODS
  The primarily cultured adrenocortical cells were quantitatively divided into control group (SO), pancreatitis group (SAP), PDTC drug control group (SO+PDTC), PDTC intervention group (SAP+PDTC), 5-AIQ drug control group (SO+ 5-AIQ) and 5-AIQ intervention group (SAP+5-AIQ). The SAP and 2 intervention groups were stimulated with the sera of SAP rats. Then corresponding drugs were added and culture continued for 12 hours. The corticosterone levels and PARP/NF-κB expression were observed for each group.
  
  
  RESULTS
  Adrenal cells in vitro cultured were round or oval, had secretory granules and could be stained by 3β-hydroxysteroid dehydrogenase antibody. The adherence rate was 60% after 48-hour culturing. The corticosterone level of SAP group was significantly lower than that of SO group [ (216.4 ± 15.7) vs (294.8 ± 16.3) µg/L, P < 0.05]. The 2 intervention groups were (258.6 ± 19.0) and (264.3 ± 18.2) µg/L respectively. These two values were higher than those of SAP group (P < 0.05), but lower than those of SO group (P < 0.05). With regards to the expression of PARP-1, the SAP and PDTC intervention groups were higher than SO group while 5-AIQ intervention group was significantly lower than SAP and PDTC intervention groups, but higher than SO and drug control groups. The expression of NF-κB in SAP group was higher than that in SO group. Two intervention groups were lower than SAP group, but higher than SO and drug control groups.
  
  
  CONCLUSIONS
  The pathway of PARP/NF-κB participates in adrenal damage of SAP rats. To a certain extent, the uses of 5-AIQ and PDTC may alleviate adrenal damage.
authors.affiliation: Department of Heart and Vascular Center, Beijing Anzhan Hospital Affiliated Capital University of Medicine, Beijing 100029, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.forename: Wen-Yi
authors.initials: WY
authors.lastname: Guo
authors.forename: Jia
authors.initials: J
authors.lastname: Yu
authors.forename: Kai-liang
authors.initials: KL
authors.lastname: Zhao
authors.forename: Qiao
authors.initials: Q
authors.lastname: Shi
authors.forename: Teng
authors.initials: T
authors.lastname: Zuo
authors.affiliation: Department of General Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, China.Email: sate.llite@163.com.
authors.forename: Wei-xing
authors.initials: WX
authors.lastname: Wang
mesh_terms: D000208:Acute Disease; D000302:Adrenal Cortex / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D007546:Isoquinolines / Q000494:pharmacology; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D010195:Pancreatitis / Q000378:metabolism*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D011392:Proline / Q000031:analogs & derivatives / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013859:Thiocarbamates / Q000494:pharmacology
chemical_list: C411425:5-aminoisoquinolinone; D007546:Isoquinolines; D016328:NF-kappa B; D013859:Thiocarbamates; C066229:prolinedithiocarbamate; D011392:Proline; D011065:Poly(ADP-ribose) Polymerases