title: Polymorphism of leukocyte and erythrocyte antigens in chronic kidney disease patients in southern Brazil.
abstract: We investigated the polymorphism of human leukocyte antigens (HLA) and Duffy erythrocyte antigens in chronic kidney disease (CKD) patients in southern Brazil. One hundred and eighty-three CKD patients, over 18 years old, on hemodialysis, were included. HLA-A, -B and -DRB1 typing was performed using the LABType®SSO (One Lambda, Inc.). Duffy phenotypes were determined by gel column agglutination using anti-Fy(a) and anti-Fy(b) monoclonal anti-sera. The patients' predominant ages ranged between 51 and 70 years (43%) and the predominant gender, ethnic group and dialysis period were, respectively, male (62%), white (62%) and 1-3 years (40%). The highest and lowest frequencies of Duffy phenotypes were Fy(a+b+) and Fy(a-b-), respectively. Nineteen HLA-A, 30 HLA-B and 13 HLA-DRB1 allele groups were identified. The most frequent HLA allele groups were HLA-A*01, -A*02, -A*03, -A*11, -A*24; HLA-B*07, -B*15, -B*35, -B*44, -B*51; HLA-DRB1*03, -DRB1*04, -DRB1*07, -DRB1*11 and -DRB1*13. Statistically significant differences were observed in the Duffy and HLA polymorphisms compared between CKD patients and healthy subjects. The Fy(a+b-) phenotype (p<0.0001, OR = 2.56, 95% CI = 1.60-4.07) was the most frequent in the patients (p<0.05), and the Fy(a+b+) phenotype (p = 0.0039, OR = 1.71, 95% CI = 1.18-2.51) was the most frequent in the healthy subjects in the same region of Paraná state (p<0.05). Regarding HLA, the HLA-B*42, -B*45, -B*51 and -DRB1*03 allele groups were the most frequent in the patients (p<0.05), and the HLA-B*44 allele group was the most frequent in the healthy subjects in the same region of Brazil (p<0.05). The polymorphism of these two markers among CKD patients in southern Brazil and healthy subjects of other studies, suggests that these markers might be involved with CKD development. Further studies should be undertaken to analyze the markers' influence on CKD and the long-term results from kidney transplantation.
authors.affiliation: Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
authors.forename: Roger Haruki
authors.initials: RH
authors.lastname: Yamakawa
authors.affiliation: Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
authors.forename: Patricia Keiko
authors.initials: PK
authors.lastname: Saito
authors.affiliation: Departamento de Estatística, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
authors.forename: Waldir Veríssimo
authors.initials: WV
authors.lastname: da Silva Junior
authors.affiliation: Departamento de Biologia Molecular, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, São Paulo, Brazil.
authors.forename: Luiz Carlos
authors.initials: LC
authors.lastname: de Mattos
authors.affiliation: Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
authors.forename: Sueli Donizete
authors.initials: SD
authors.lastname: Borelli
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D001938:Brazil; D004375:Duffy Blood-Group System / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006680:HLA Antigens / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D011110:Polymorphism, Genetic; D051436:Renal Insufficiency, Chronic / Q000235:genetics* / Q000628:therapy; D055815:Young Adult
chemical_list: D004375:Duffy Blood-Group System; D006680:HLA Antigens