title: Effects of thermal stress and nickel exposure on biomarkers responses in Mytilus galloprovincialis (Lam).
abstract: The present work aimed to assess the Mytilus galloprovincialis digestive gland biomarkers responses to nickel (Ni) exposure along with a heat stress gradient. Mussels were exposed to a sublethal dose of nickel (13 μM) along with a temperature gradient (18 °C, 20 °C, 22 °C, 24 °C and 26 °C) for 4 days. Metallothionein (MTs) content was assessed as specific response to metals. Catalase (CAT), glutathione S-transferase (GST) activities and malondialdehyde (MDA) were measured as biomarkers of oxidative stress and lipid peroxidation. The cholinergic system was monitored using the acetylcholinesterase activity (AChE). Moreover, Ni uptakes along with the exposure temperatures were assessed. A correlation matrix (CM) between the investigated biomarkers and the exposure temperatures and a Principal Component Analysis (PCA) were achieved. Our data showed a negative effect of temperature increase on mussel's antioxidant and detoxification response to Ni exposure being more pronounced in animals exposed to the 24 °C and 26 °C.
authors.affiliation: Laboratory of Biochemistry and Environmental Toxicology, ISA, Chott-Mariem, 4042 Sousse, Tunisia.
authors.forename: Hajer
authors.initials: H
authors.lastname: Attig
authors.affiliation: Laboratory of Biochemistry and Environmental Toxicology, ISA, Chott-Mariem, 4042 Sousse, Tunisia.
authors.forename: Naouel
authors.initials: N
authors.lastname: Kamel
authors.affiliation: Department of Environmental and Life Sciences, University of Piemonte Orientale Amedeo Avogadro, Via Bellini 25 G, 15100 Alessandria, Italy.
authors.forename: Susanna
authors.initials: S
authors.lastname: Sforzini
authors.affiliation: Department of Environmental and Life Sciences, University of Piemonte Orientale Amedeo Avogadro, Via Bellini 25 G, 15100 Alessandria, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Dagnino
authors.affiliation: Laboratory of Biochemistry and Environmental Toxicology, ISA, Chott-Mariem, 4042 Sousse, Tunisia.
authors.forename: Jebali
authors.initials: J
authors.lastname: Jamel
authors.affiliation: Laboratory of Biochemistry and Environmental Toxicology, ISA, Chott-Mariem, 4042 Sousse, Tunisia.
authors.forename: Hamadi
authors.initials: H
authors.lastname: Boussetta
authors.affiliation: Department of Environmental and Life Sciences, University of Piemonte Orientale Amedeo Avogadro, Via Bellini 25 G, 15100 Alessandria, Italy.
authors.forename: Aldo
authors.initials: A
authors.lastname: Viarengo
authors.affiliation: Laboratory of Biochemistry and Environmental Toxicology, ISA, Chott-Mariem, 4042 Sousse, Tunisia; Department of Environmental and Life Sciences, University of Piemonte Orientale Amedeo Avogadro, Via Bellini 25 G, 15100 Alessandria, Italy. Electronic address: m_banni@yahoo.fr.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Banni
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D004789:Enzyme Activation / Q000187:drug effects; D006358:Hot Temperature; D049878:Mytilus / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology*; D009532:Nickel / Q000633:toxicity*; D013312:Stress, Physiological; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D014874:Water Pollutants, Chemical; D009532:Nickel
keywords: Biomarker response; Global warming; Heat stress; Mussels; Nickel; Oxidative stress