title: Infectious bursal disease virus influences the transcription of chicken γc and γc family cytokines during infection.
abstract: Infectious bursal disease virus (IBDV) infection causes immunodeficiency in chickens. To understand cell-mediated immunity during IBDV infection, this study perform a detailed analysis of chicken γc chain (chCD132) and γc family cytokines, including interleukins 2, 4, 7, 9, and 15. The mouse anti-chCD132 monoclonal antibody (mAb) was first generated by the E.coli-expressed γc protein. Immunofluorescence assay further showed that γc was a protein located with the anti-chCD132 mAb on the surface of chicken's splenic mononuclear cells. Real-time quantitative RT-PCR revealed that the chCD132 mRNA transcript was persistently downregulated in embryo fibroblasts, spleen and thymus of chickens infected with IBDV. Correspondingly during IBDV infection, the transcription of five γc family cytokines was downregulated in the thymus and presented an imbalance in the spleen. Fluorescence-activated cell sorting analyses also indicated that the percentage of CD132(+)CD8(+) T cells linearly decreased in the bursa of IBDV-infected chickens. These results confirmed that IBDV infection disturbed the in vivo balance of CD132 and γc family cytokine expression and that IBDV-induced immunodeficiency involved cellular networks related to the γc family.
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Sanying
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Qiaoyang
authors.initials: Q
authors.lastname: Teng
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Lu
authors.initials: L
authors.lastname: Jia
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Sun
authors.affiliation: College of Animal Sciences and Technology, Zhejiang A&F University, Lin'an, Zhejiang, People's Republic of China.
authors.forename: Yongping
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China ; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Jiyong
authors.initials: J
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D018175:Birnaviridae Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D002645:Chickens / Q000276:immunology / Q000821:virology*; D016207:Cytokines / Q000235:genetics*; D005347:Fibroblasts / Q000821:virology; D005786:Gene Expression Regulation; D007243:Infectious bursal disease virus / Q000472:pathogenicity / Q000502:physiology*; D053631:Interleukin Receptor Common gamma Subunit / Q000235:genetics* / Q000276:immunology; D007700:Kinetics; D010641:Phenotype; D011201:Poultry Diseases / Q000235:genetics* / Q000276:immunology / Q000821:virology; D013601:T-Lymphocytes / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D000911:Antibodies, Monoclonal; D016207:Cytokines; D053631:Interleukin Receptor Common gamma Subunit