title: Increased Th17/Treg ratio in chronic liver GVHD.
abstract: The contribution of Th17 cells to chronic GVHD (cGVHD) has been demonstrated in cGVHD mouse models. However, their contribution to human liver cGVHD remains unclear. We evaluated Th17 cells in biopsies from a cohort of 17 patients with liver cGVHD. We observed a significant increase in Th17 cells in the liver of patients with cGVHD, as demonstrated by an increase in CCR6+, CD161+ and RORγt+ T cells (P=0.03, P=0.0001 and P=0.03, respectively). We also assessed the presence of Th1 and regulatory (Treg) T cells: the numbers of Th1 and Treg cells were very low, with no difference between the two groups (P=0.88 and P=0.12, respectively). Furthermore, Th17/Th1 and Th17/Treg ratios were significantly increased in the liver of patients with liver cGVHD (P=0.005 and P=0.002, respectively). This study provides evidence for an infiltration by Th17 cells in the liver of patients with cGVHD and an increased Th17/Treg ratio, suggesting a defect in the regulatory mechanism driven by Treg cells or an inappropriate activation of effectors cells, especially Th17 cells, or both mechanisms, in human liver cGVHD.
authors.affiliation: 1] CRNCA, UMR 892 INSERM - 6299 CNRS, Nantes, France [2] Service d'Hématologie Clinique, Centre Hospitalier et Universitaire (CHU), Université de Nantes, Nantes, France.
authors.forename: F
authors.initials: F
authors.lastname: Malard
authors.affiliation: 1] EA4273 Biometadys, Faculté de médecine, Université de Nantes, Nantes, France [2] Service d'Anatomie et Cytologie Pathologique, CHU de Nantes, Nantes, France.
authors.forename: C
authors.initials: C
authors.lastname: Bossard
authors.affiliation: 1] CRNCA, UMR 892 INSERM - 6299 CNRS, Nantes, France [2] Service d'Hématologie Clinique, Centre Hospitalier et Universitaire (CHU), Université de Nantes, Nantes, France.
authors.forename: E
authors.initials: E
authors.lastname: Brissot
authors.affiliation: Service d'Hématologie Clinique, Centre Hospitalier et Universitaire (CHU), Université de Nantes, Nantes, France.
authors.forename: P
authors.initials: P
authors.lastname: Chevallier
authors.affiliation: Service d'Hématologie Clinique, Centre Hospitalier et Universitaire (CHU), Université de Nantes, Nantes, France.
authors.forename: T
authors.initials: T
authors.lastname: Guillaume
authors.affiliation: Service d'Hématologie Clinique, Centre Hospitalier et Universitaire (CHU), Université de Nantes, Nantes, France.
authors.forename: J
authors.initials: J
authors.lastname: Delaunay
authors.affiliation: 1] EA4273 Biometadys, Faculté de médecine, Université de Nantes, Nantes, France [2] Service d'Anatomie et Cytologie Pathologique, CHU de Nantes, Nantes, France.
authors.forename: J-F
authors.initials: JF
authors.lastname: Mosnier
authors.affiliation: 1] CRNCA, UMR 892 INSERM - 6299 CNRS, Nantes, France [2] Service d'Hématologie Clinique, Centre Hospitalier et Universitaire (CHU), Université de Nantes, Nantes, France [3] Centre d'Investigation Clinique en Cancérologie (CI2C), Nantes, France.
authors.forename: P
authors.initials: P
authors.lastname: Moreau
authors.affiliation: CRNCA, UMR 892 INSERM - 6299 CNRS, Nantes, France.
authors.forename: M
authors.initials: M
authors.lastname: Grégoire
authors.affiliation: 1] INSERM UMR 1098, Besançon, France [2] Université de Franche-Comté, Besançon, France [3] EFS Bourgogne Franche-Comté, 25020 Besançon Cedex, Besançon, France [4] Centre d'Investigation Clinique en Biothérapie CBT506, Plateforme de Biomonitoring, Besançon, France.
authors.forename: B
authors.initials: B
authors.lastname: Gaugler
authors.affiliation: 1] CRNCA, UMR 892 INSERM - 6299 CNRS, Nantes, France [2] Service d'Hématologie Clinique, Centre Hospitalier et Universitaire (CHU), Université de Nantes, Nantes, France.
authors.forename: M
authors.initials: M
authors.lastname: Mohty
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D001706:Biopsy; D005260:Female; D006086:Graft vs Host Disease / Q000276:immunology* / Q000473:pathology; D006801:Humans; D008107:Liver Diseases / Q000276:immunology* / Q000473:pathology; D008297:Male; D051379:Mice; D008875:Middle Aged; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000473:pathology*; D058504:Th17 Cells / Q000276:immunology / Q000473:pathology*