title: Galactolipid synthesis in chromoplasts in vitro : Specificities of acyl-and galactosyltransferases.
abstract: Isolated chromoplasts from Narcissus pseudonarcissus flowers contain: a fatty acid synthesizing system; acyl-CoA synthetase (EC 6.2.1.3); glycero-phosphate acyltransferase (EC 2.3.1.15); acylglycero-phosphate acyltransferase; phosphatidate phosphatase (EC 3.1.3.4); diacylglycerol galactosyltransferase (EC 2.4.1.46); and diacylgalactosylglycerol galactosyltransferase, i.e. all enzymatic activities necessary for the synthesis of diacylgalactosylglycerol and diacylgalabiosylglycerol from acetate, HCO - (3) , sn-glycerol 3-phosphate, and UDP-D-galactose. Diacylgalactosylglycerol and diacylgalabiosylglycerol, however, are synthesized from these precursors to only a very low extent in an in vitro system. This is attributed to a specificity of diacylglycerol galactosyltransferase for highly unsaturated diacylglycerols. Specificities of acyltransferase reactions were also found.
authors.affiliation: Lehrstuhl für Zellbiologie, Institut für Biologie II der Universität, Schänzlestraße, D-7800, Freiburg/Br., Federal Republic of Germany.
authors.forename: B
authors.initials: B
authors.lastname: Liedvogel
authors.forename: H
authors.initials: H
authors.lastname: Kleinig