title: Serum thiamine concentration and oxidative stress as predictors of mortality in patients with septic shock.
abstract: OBJECTIVE
  The purpose of the study is to determine the influence of serum thiamine, glutathione peroxidase (GPx) activity, and serum protein carbonyl concentrations in hospital mortality in patients with septic shock.
  
  
  METHODS
  This prospective study included all patients with septic shock on admission or during intensive care unit (ICU) stay, older than 18 years, admitted to 1 of the 3 ICUs of the Botucatu Medical School, from January to August 2012. Demographic information, clinical evaluation, and blood sample were taken within the first 72 hours of the patient's admission or within 72 hours after septic shock diagnosis for serum thiamine, GPx activity, and protein carbonyl determination.
  
  
  RESULTS
  One hundred eight consecutive patients were evaluated. The mean age was 57.5 ± 16.0 years, 63% were male, 54.6% died in the ICU, and 71.3% had thiamine deficiency. Thiamine was not associated with oxidative stress. Neither vitamin B1 levels nor the GPx activity was associated with outcomes in these patients. However, protein carbonyl concentration was associated with increased mortality.
  
  
  CONCLUSIONS
  In patients with septic shock, oxidative stress was associated with mortality. On the other hand, thiamine was not associated with oxidative stress or mortality in these patients.
authors.affiliation: Department of Internal Medicine, Botucatu Medical School, UNESP-Univ Estadual Paulista, Botucatu, Brazil.
authors.forename: Nara Aline
authors.initials: NA
authors.lastname: Costa
authors.affiliation: Department of Internal Medicine, Botucatu Medical School, UNESP-Univ Estadual Paulista, Botucatu, Brazil.
authors.forename: Ana Lúcia
authors.initials: AL
authors.lastname: Gut
authors.affiliation: Department of Internal Medicine, Botucatu Medical School, UNESP-Univ Estadual Paulista, Botucatu, Brazil.
authors.forename: Mariana
authors.initials: M
authors.lastname: de Souza Dorna
authors.affiliation: Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Science, University of São Paulo, São Paulo, Brazil.
authors.forename: José Alexandre Coelho
authors.initials: JA
authors.lastname: Pimentel
authors.affiliation: Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Science, University of São Paulo, São Paulo, Brazil.
authors.forename: Silvia Maria Franciscato
authors.initials: SM
authors.lastname: Cozzolino
authors.affiliation: Department of Internal Medicine, Botucatu Medical School, UNESP-Univ Estadual Paulista, Botucatu, Brazil.
authors.forename: Paula Schmidt
authors.initials: PS
authors.lastname: Azevedo
authors.affiliation: Chemistry and Biochemistry Department, Institute of Biological Sciences, UNESP-Univ Estadual Paulista, Botucatu, Brazil.
authors.forename: Ana Angélica Henrique
authors.initials: AA
authors.lastname: Fernandes
authors.affiliation: Department of Internal Medicine, Botucatu Medical School, UNESP-Univ Estadual Paulista, Botucatu, Brazil.
authors.forename: Leonardo Antonio Mamede
authors.initials: LA
authors.lastname: Zornoff
authors.affiliation: Department of Internal Medicine, Botucatu Medical School, UNESP-Univ Estadual Paulista, Botucatu, Brazil.
authors.forename: Sergio Alberto Rupp
authors.initials: SA
authors.lastname: de Paiva
authors.affiliation: Department of Internal Medicine, Botucatu Medical School, UNESP-Univ Estadual Paulista, Botucatu, Brazil. Electronic address: minicucci@fmb.unesp.br.
authors.forename: Marcos Ferreira
authors.initials: MF
authors.lastname: Minicucci
mesh_terms: D015415:Biomarkers / Q000097:blood; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood*; D017052:Hospital Mortality; D006801:Humans; D007362:Intensive Care Units; D007902:Length of Stay; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D011446:Prospective Studies; D050050:Protein Carbonylation; D012772:Shock, Septic / Q000097:blood / Q000401:mortality*; D013831:Thiamine / Q000097:blood*
chemical_list: D015415:Biomarkers; D005979:Glutathione Peroxidase; D013831:Thiamine
keywords: Glutathione peroxidase; Mortality; Protein carbonyl; Septic shock; Thiamine