title: Chemical inhibition of cell wall formation and cytokinesis, but not of nuclear division, in protoplasts of Nicotiana tabacum L. cultivated in vitro.
abstract: The effect of cytochalasin B, colchicine, coumarin and 2,6-dichlorobenzonitrile on cell wall formation and cellular division was studied by light and electron microscopy with tobacco mesophyll protoplasts cultivated in vitro. 2,6-dichlorobenzonitrile was found to be the most effective and reversible inhibitor of cell wall formation. The other inhibitors caused irreversible damage and/or inhibited mitosis. In protoplasts cultivated in the presence of 2,6-dichlorobenzonitrile the total inhibition of cell wall formation had no effect on nuclear division, but cytokinesis was totally inhibited so that multinucleate protoplasts were obtained.
authors.affiliation: Institut für Botanik und Lehrstuhl für Zellenlehre der Universität Heidelberg, Im Neuenheimer Feld 230, D-6900, Heidelberg, Federal Republic of Germany.
authors.forename: Y
authors.initials: Y
authors.lastname: Meyer
authors.forename: W
authors.initials: W
authors.lastname: Herth