title: Immobilization of horseradish peroxidase in phospholipid-templated titania and its applications in phenolic compounds and dye removal.
abstract: In this study, horseradish peroxidase (HRP) was encapsulated in phospholipid-templated titania particles through the biomimetic titanification process and used for the treatment of wastewater polluted with phenolic compounds and dye. The encapsulated HRP exhibited improved thermal stability, a wide range of pH stability and high tolerance against inactivating agents. It was observed an increase in Km value for the encapsulated HRP (8.21 mM) when compared with its free counterpart. For practical applications in the removal of phenolic compounds and dye by the encapsulated HRP, the removal efficiency for phenol, 2-chlorophenol, Direct Black-38 were 92.99%, 87.97%, and 79.72%, respectively, in the first treatment cycle. Additionally, the encapsulated HRP showed better removal efficiency than free HRP and a moderately good capability of reutilization.
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China. Electronic address: jgao@hebut.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China.
authors.forename: Liya
authors.initials: L
authors.lastname: Zhou
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: He
mesh_terms: D001391:Azo Compounds; D002384:Catalysis; D002733:Chlorophenols; D004396:Coloring Agents; D004339:Drug Compounding; D052918:Environmental Restoration and Remediation / Q000379:methods; D004800:Enzymes, Immobilized; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D019800:Phenol; D010743:Phospholipids / Q000502:physiology; D014025:Titanium / Q000737:chemistry*
chemical_list: D001391:Azo Compounds; D002733:Chlorophenols; D004396:Coloring Agents; D004800:Enzymes, Immobilized; D010743:Phospholipids; C009495:titanium dioxide; D019800:Phenol; C015844:direct black 3; D014025:Titanium; D006735:Horseradish Peroxidase; C030683:2-chlorophenol
keywords: Dye; Enzyme encapsulation; HRP; Horseradish peroxidase; Phenolic compound; Phospholipid-templated titania; horseradish peroxidase