title: Isolation of a fruit ripening-related tonoplast aquaporin (GjTIP) gene from Gardenia jasminoides.
abstract: Aquaporins are membrane water channels that play critical roles in controlling the water content of cells and tissues. In this work, a full-length cDNA encoding putative aquaporins was isolated from Gardenia jasminoides fruit cDNA library. The GjTIP cDNA is 1188 bp, contains a predicted 774 bp open reading frame that encodes 257 amino acids. A phylogenetic analysis conducted with previously characterized aquaporins from other plant species indicates that the cDNA encode putative tonoplast aquaporins (TIPs), and proposed that GjTIP has a tendency to be a mixed function aquaporin similar to the TIP1s from Arabidopsis and Gossypium raimondii. A typical "hourglasses" three-dimensional model of GjTIP was built. The expression of the GjTIP transcripts at fruits during maturation was conducted by RT-PCR analysis. The data revealed that the transcript levels of GjTIP have increased during fruit maturation.
authors.affiliation: School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, 510006 Peoples Republic of China.
authors.forename: Lan
authors.initials: L
authors.lastname: Gao
authors.affiliation: School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, 510006 Peoples Republic of China.
authors.forename: Yi-Jun
authors.initials: YJ
authors.lastname: Guo
keywords: Expression; Fruits; Gardenia jasminoides; Tonoplast aquaporin