title: Rapid diagnosis of tuberculosis by identification of Antigen 85 in mycobacterial culture system.
abstract: The standard culture for identification of Mycobacterium tuberculosis takes a long time to perform. We introduce here a method for fast identification of M. tuberculosis in mycobacterial culture system. Antibodies to Antigen (Ag) 85 of M. tuberculosis were produced and subsequently used to develop enzyme-linked immunosorbent assay (ELISA) for detecting Ag85 in the culture filtrate. By this detection, rapid tuberculosis (TB) diagnosis was achieved in comparison to the standard culture system with 89.6% sensitivity and 94% specificity. We thus suggest a new TB diagnosis strategy in which clinical samples are cultured in mycobacteria liquid culture medium. The culture filtrates are taken for detection of the Ag85 by ELISA. Using this strategy, 25%, 50%, 80%, and 90% of TB patients will be detected within day 3, week 1, 2, and 4, respectively. The established assay will enable a faster diagnosis of TB, leading to more efficient treatment of TB patients and control of disease transmission.
authors.affiliation: Division of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Ponrut
authors.initials: P
authors.lastname: Phunpae
authors.affiliation: Office of Prevention Disease Control Region 10, Chiang Mai 50000, Thailand.
authors.forename: Sakarin
authors.initials: S
authors.lastname: Chanwong
authors.affiliation: Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand; Biomedical Technology Research Center, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency at the Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Chatchai
authors.initials: C
authors.lastname: Tayapiwatana
authors.affiliation: School of Medicine, Mae Fah Luang University, Chiang Rai 57100, Thailand.
authors.forename: Napaporn
authors.initials: N
authors.lastname: Apiratmateekul
authors.affiliation: Biomedical Technology Research Center, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency at the Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Anupong
authors.initials: A
authors.lastname: Makeudom
authors.affiliation: Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand; Biomedical Technology Research Center, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency at the Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. Electronic address: watchara.k@cmu.ac.th.
authors.forename: Watchara
authors.initials: W
authors.lastname: Kasinrerk
mesh_terms: D000217:Acyltransferases / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000302:isolation & purification*; D015153:Blotting, Western; D003470:Culture Media / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000737:chemistry; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis*
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D003470:Culture Media; D011994:Recombinant Proteins; D000217:Acyltransferases; C121433:antigen 85B, Mycobacterium tuberculosis
keywords: Ag85 complex; Diagnosis; ELISA; M. tuberculosis; Mycobacterial culture system; Tuberculosis