title: Genetic and phenotypically flexible components of seasonal variation in immune function.
abstract: Animals cope with seasonal variation in environmental factors by adjustments of physiology and life history. When seasonal variation is partly predictable, such adjustments can be based on a genetic component or be phenotypically flexible. Animals have to allocate limited resources over different demands, including immune function. Accordingly, immune traits could change seasonally, and such changes could have a genetic component that differs between environments. We tested this hypothesis in genotypically distinct groups of a widespread songbird, the stonechat (Saxicola torquata). We compared variation in immunity during 1 year in long-distance migrants, short-distance migrants, tropical residents and hybrids in a common garden environment. Additionally, we investigated phenotypically flexible responses to temperature by applying different temperature regimes to one group. We assessed constitutive immunity by measuring hemagglutination, hemolysis, haptoglobin and bactericidal ability against Escherichia coli and Staphylococcus aureus. Genotypic groups differed in patterns of variation of all measured immune indices except haptoglobin. Hybrids differed from, but were rarely intermediate to, parental subspecies. Temperature treatment only influenced patterns of hemolysis and bactericidal ability against E. coli. We conclude that seasonal variation in constitutive immunity has a genetic component, that heredity does not follow simple Mendelian rules, and that some immune measures are relatively rigid while others are more flexible. Furthermore, our results support the idea that seasonal variability in constitutive immunity is associated with variability in environment and annual-cycle demands. This study stresses the importance of considering seasonal variation in immune function in relation to the ecology and life history of the organism of interest.
authors.affiliation: Animal Ecology Group, Centre for Ecological and Evolutionary Studies, University of Groningen, PO Box 11103, 9700 CC, Groningen, The Netherlands.
authors.forename: M A
authors.initials: MA
authors.lastname: Versteegh
authors.forename: B
authors.initials: B
authors.lastname: Helm
authors.forename: E J
authors.initials: EJ
authors.lastname: Kleynhans
authors.forename: E
authors.initials: E
authors.lastname: Gwinner
authors.forename: B I
authors.initials: BI
authors.lastname: Tieleman
mesh_terms: D025041:Animal Migration / Q000502:physiology; D000818:Animals; D002980:Climate; D004926:Escherichia coli / Q000276:immunology; D007109:Immunity / Q000235:genetics* / Q000502:physiology*; D046109:Passeriformes / Q000276:immunology*; D010641:Phenotype; D012621:Seasons; D013211:Staphylococcus aureus / Q000276:immunology; D013696:Temperature
keywords: Common garden; Constitutive immunity; Genetic components; Phenotypic flexibility; Seasonal variation; Stonechats