title: Intervention in cyclophosphamide induced oxidative stress and DNA damage by a flavonyl-thiazolidinedione based organoselenocyanate and evaluation of its efficacy during adjuvant therapy in tumor bearing mice.
abstract: A novel flavonyl-thiazolidinedione based organoselenocyanate compound was synthesized and established as nontoxic at the doses of 2.5 and 5 mg/kg b.w. in mice. Oral administration of the compound in combination with cyclophosphamide (CP) resulted in an improved therapeutic efficacy which was mostly evidenced in terms of tumor burden and protection of normal cells. The adjuvant therapy was proved to be immensely significant in increasing the mean survivability of the tumor bearing hosts. Reduction in the tumor volume was manifested through the induction of apoptosis and generation of ROS in transformed cells. Moreover, the organoselenium compound could efficiently suppress CP-induced DNA damage, chromosomal aberration, hepatic damage and enhanced the activities of various antioxidant enzymes in normal cells.
authors.affiliation: Chittaranjan National Cancer Institute, Department of Cancer Chemoprevention, 37, S. P. Mukherjee Road, Kolkata 700 026, West Bengal, India.
authors.forename: Somnath Singha
authors.initials: SS
authors.lastname: Roy
authors.affiliation: Chittaranjan National Cancer Institute, Department of Cancer Chemoprevention, 37, S. P. Mukherjee Road, Kolkata 700 026, West Bengal, India.
authors.forename: Pramita
authors.initials: P
authors.lastname: Chakraborty
authors.affiliation: Chittaranjan National Cancer Institute, Department of Cancer Chemoprevention, 37, S. P. Mukherjee Road, Kolkata 700 026, West Bengal, India. Electronic address: sudinb19572004@yahoo.co.in.
authors.forename: Sudin
authors.initials: S
authors.lastname: Bhattacharya
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002286:Carcinoma, Ehrlich Tumor / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D045744:Cell Line, Tumor; D017024:Chemotherapy, Adjuvant; D003485:Cyanates / Q000737:chemistry; D003520:Cyclophosphamide / Q000633:toxicity*; D004249:DNA Damage; D005260:Female; D047309:Flavones / Q000737:chemistry*; D006454:Hemoglobins / Q000032:analysis; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008111:Liver Function Tests; D051379:Mice; D016566:Organoselenium Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use; D018384:Oxidative Stress / Q000187:drug effects*; D018036:Selenium Compounds / Q000737:chemistry; D045162:Thiazolidinediones / Q000737:chemistry*; D014018:Tissue Distribution
chemical_list: D000975:Antioxidants; D003485:Cyanates; D047309:Flavones; D006454:Hemoglobins; D016566:Organoselenium Compounds; D018036:Selenium Compounds; D045162:Thiazolidinediones; C053721:selenocyanic acid; D003520:Cyclophosphamide
keywords: Biodistribution; Chemoenhancement; Chemoprotection; Cyclophosphamide; Organoselenium; ROS