title: On-site enzymes produced from Trichoderma reesei RUT-C30 and Aspergillus saccharolyticus for hydrolysis of wet exploded corn stover and loblolly pine.
abstract: Cellulase production by two filamentous fungi Trichoderma reesei RUT-C30 and novel fungal strain, Aspergillus saccharolyticus on pretreated corn stover was investigated. Cellulase production was followed by the hydrolysis of two feedstocks, wet-exploded corn stover (WECS) and wet-exploded loblolly pine (WELP) by on-site produced enzyme cocktails containing cellulase from T. reesei RUT-C30 and β-glucosidase from A. saccharolyticus. The sugar yields using the on-site enzyme cocktails were compared with commercial enzymes preparations, Celluclast 1.5L and Novozym 188 at two substrate concentrations, 5% and 10% (w/w) and enzyme loading at 5 and 15 FPU/g glucan for WECS and WELP. The highest sugar yields were obtained at 5% (w/w) substrate concentration and 15 FPU/g glucan for both feedstocks. Glucose yields of 81% and 88% were obtained from on-site and commercial enzymes, respectively using WECS as feed stock. The sugar yields were 55% and 58% for WELP samples hydrolyzed with on-site and commercial enzymes, respectively.
authors.affiliation: Bioproducts, Sciences and Engineering Laboratory (BSEL), Washington State University Richland, WA 99354, United States.
authors.forename: Vandana
authors.initials: V
authors.lastname: Rana
authors.affiliation: Bioproducts, Sciences and Engineering Laboratory (BSEL), Washington State University Richland, WA 99354, United States.
authors.forename: Anahita D
authors.initials: AD
authors.lastname: Eckard
authors.affiliation: Bioproducts, Sciences and Engineering Laboratory (BSEL), Washington State University Richland, WA 99354, United States.
authors.forename: Philip
authors.initials: P
authors.lastname: Teller
authors.affiliation: Bioproducts, Sciences and Engineering Laboratory (BSEL), Washington State University Richland, WA 99354, United States. Electronic address: bka@wsu.edu.
authors.forename: Birgitte K
authors.initials: BK
authors.lastname: Ahring
mesh_terms: D001230:Aspergillus / Q000201:enzymology*; D002480:Cellulase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D010100:Oxygen / Q000032:analysis; D041603:Pinus taeda / Q000187:drug effects / Q000378:metabolism*; D013696:Temperature; D014242:Trichoderma / Q000201:enzymology*; D014867:Water / Q000494:pharmacology*; D003313:Zea mays / Q000187:drug effects / Q000378:metabolism*
chemical_list: D014867:Water; D002480:Cellulase; D010100:Oxygen
keywords: A. saccharolyticus; Cellulase production; T. reesei RUT-C30; Wet explosion; β-Glucosidase