title: [Analysis of difference of proteins of gastric cancer tissue and adjacent normal tissue by capillary electrophoresis with laser-induced fluorescence detection].
abstract: Gastric cancer is one of the familiar malignant tumors in clinical study. Research on tumor biomarkers has increased noticeably in recent years. An experimental method of protein separation by capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) was established and improved. A series of effects on capillary electrophoresis (CE) were studied, such as the dynamic coating method of capillary, concentration of polyethylene oxide (PEO) as sieving medium, pH of running buffer, separation voltage, temperature and fluorescent dye. Then the optimized method was established for the determination of gastric cancer tissue and adjacent normal tissue. According to the analysis of the protein fingerprints, the results showed that the similarity of protein was up to 0.8. The molecular masses of differential proteins were almost between 50,000 Da and 100,000 Da. It indicated that these proteins might be potential biomarkers for cancer diagnosis which may reduce the searching scope. By statistical analysis of histological classification and electrophoresis peak numbers, this method was demonstrated reliably, and had the potential for clinical applications.
authors.affiliation: PLA Key Laboratory of the Plateau of the Environmental Damage Control, Base of Clinic Pharmacology, Lanzhou General Hospital, Lanzhou Command, Lanzhou 730050, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Hao
authors.affiliation: PLA Key Laboratory of the Plateau of the Environmental Damage Control, Base of Clinic Pharmacology, Lanzhou General Hospital, Lanzhou Command, Lanzhou 730050, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.affiliation: PLA Key Laboratory of the Plateau of the Environmental Damage Control, Base of Clinic Pharmacology, Lanzhou General Hospital, Lanzhou Command, Lanzhou 730050, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yin
authors.affiliation: PLA Key Laboratory of the Plateau of the Environmental Damage Control, Base of Clinic Pharmacology, Lanzhou General Hospital, Lanzhou Command, Lanzhou 730050, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Xie
authors.affiliation: PLA Key Laboratory of the Plateau of the Environmental Damage Control, Base of Clinic Pharmacology, Lanzhou General Hospital, Lanzhou Command, Lanzhou 730050, China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Li
authors.affiliation: PLA Key Laboratory of the Plateau of the Environmental Damage Control, Base of Clinic Pharmacology, Lanzhou General Hospital, Lanzhou Command, Lanzhou 730050, China.
authors.forename: Zhengping
authors.initials: Z
authors.lastname: Jia
mesh_terms: D019075:Electrophoresis, Capillary; D005456:Fluorescent Dyes; D006801:Humans; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000032:analysis*; D010449:Peptide Mapping; D013050:Spectrometry, Fluorescence; D013274:Stomach Neoplasms
chemical_list: D005456:Fluorescent Dyes; D009363:Neoplasm Proteins