title: The morphology, proliferation rate, and population doubling time factor of adipose-derived mesenchymal stem cells cultured on to non-aqueous SiO2, TiO2, and hybrid sol-gel-derived oxide coatings.
abstract: In recent years, much attention has been paid to the development of tissue engineering and regenerative medicine, especially when stem cells of various sources are concerned. In addition to the interest in mesenchymal stem cells isolated from bone marrow, recently more consideration has been given to stem cells isolated from adipose tissue (AdMSCs), due to their less invasive method of collection as well as their ease of isolation and culture. However, the development of regenerative medicine requires both the application of biocompatible material and the stem cells to accelerate the regeneration. In this study, we investigated the morphology, proliferation rate index (PRi), and population doubling time factor of adipose-derived mesenchymal stem cells cultured on non-aqueous sol-gel-derived SiO2, TiO2, and SiO2/TiO2 oxide coatings. The results indicated an increase in PRi of AdMSCs when cultured on to titanium dioxide, suggesting its high attractiveness for AdMSCs. In addition, the proper morphology and the shortest doubling time of AdMSCs were observed when cultured on titanium dioxide coating.
authors.affiliation: Electron Microscopy Laboratory, University of Environmental and Life Sciences Wroclaw, ul. Kożuchowska 5B, 50-631, Wrocław, Poland; Wroclaw Research Centre EIT+, ul. Stabłowicka 147, 54-066, Wroclaw, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Marycz
authors.forename: Justyna
authors.initials: J
authors.lastname: Krzak-Roś
authors.forename: Anna
authors.initials: A
authors.lastname: Donesz-Sikorska
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Śmieszek
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D008422:Materials Testing / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D044367:Phase Transition; D051381:Rats; D017208:Rats, Wistar; D012822:Silicon Dioxide / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; C009495:titanium dioxide; D012822:Silicon Dioxide; D014025:Titanium
keywords: AdMSC; hybrid coating; oxide coating; sol-gel; stem cell