title: Zingerone attenuates lipopolysaccharide-induced acute lung injury in mice.
abstract: Zingerone, one of the active components of ginger, is a phenolic alkanone with antioxidant and anti-inflammatory properties. In the present study, we analyzed the role of zingerone against RAW 264.7 cells and acute lung injury induced by lipopolysaccharide (LPS) in mice. RAW cells or BALB/c mice were pretreated with zingerone one hour before stimulated with LPS. We found that zingerone significantly inhibited the production of LPS-induced proinflammatory cytokines in vitro and in vivo. When pretreated with zingerone, pulmonary histopathologic changes, as well as alveolar hemorrhage and neutrophil infiltration were substantially suppressed in lung tissues, with evidence of reduced myeloperoxidase (MPO) activity in murine acute lung injury model. The lung wet-to-dry weight (W/D) ratios, as the index of pulmonary edema, were markedly decreased by zingerone pretreatment. Furthermore, we demonstrated that zingerone attenuates the mitogen-activated protein kinases (MAPK) and nuclear factor-kappaB (NF-κB) signaling pathways through blocking the phosphorylation of ERK, p38/MAPK and IκBα, NF-κB/P65. These results suggest that zingerone may provide protective effects against LPS-induced ALI.
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Xianxing
authors.initials: X
authors.lastname: Xie
authors.affiliation: School of Biosciences and Biotechnology, University of Camerino, Camerino, MC 62032, Italy.
authors.forename: Shicheng
authors.initials: S
authors.lastname: Sun
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Weiting
authors.initials: W
authors.lastname: Zhong
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China; Département de Médecine Vétérinaire, Institut Supérieur des Sciences et de Médicine Vétérinaire (ISSMV) de Dalaba, Guinea.
authors.forename: Lanan Wassy
authors.initials: LW
authors.lastname: Soromou
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhou
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Wei
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Ren
authors.affiliation: College of Animal Science, Jilin University, Changchun 130062, PR China. Electronic address: ding_yu@jlu.edu.cn.
authors.forename: Yu
authors.initials: Y
authors.lastname: Ding
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D002452:Cell Count; D002460:Cell Line; D006139:Guaiacol / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D020672:I-kappa B Proteins / Q000037:antagonists & inhibitors / Q000276:immunology; D015850:Interleukin-6 / Q000276:immunology; D008070:Lipopolysaccharides; D008168:Lung / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000276:immunology; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000276:immunology; D009195:Peroxidase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D000893:Anti-Inflammatory Agents; D020672:I-kappa B Proteins; D015850:Interleukin-6; D008070:Lipopolysaccharides; D016328:NF-kappa B; C000606205:Nfkbia protein, mouse; D014409:Tumor Necrosis Factor-alpha; C577686:interleukin-6, mouse; D000072000:NF-KappaB Inhibitor alpha; C013738:zingerone; D006139:Guaiacol; D009195:Peroxidase; D020928:Mitogen-Activated Protein Kinases
keywords: Acute lung injury; Cytokines; Lipopolysaccharide; Zingerone