title: Identifying endosymbiont bacteria associated with free-living amoebae.
abstract: The association between free-living amoebae and pathogenic bacteria is an issue that has gained great importance due to the environmental and health consequences that it implies. In this paper, we analyse the techniques to follow an epidemiological study to identify associations between genera, species, genotypes and subgenotypes of amoebae with pathogenic bacteria, analysing their evolution and considering their usefulness. In this sense, we highlight the combination of microscopic and molecular techniques as the most appropriate way to obtain fully reliable results as well as the need to achieve the standardization of these techniques to allow the comparison of both environmental and clinical results.
authors.affiliation: Area of Parasitology, University of Zaragoza, Zaragoza, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Goñi
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Fernández
authors.forename: Encarnación
authors.initials: E
authors.lastname: Rubio
mesh_terms: D000656:Amoeba / Q000145:classification / Q000235:genetics / Q000382:microbiology*; D001419:Bacteria / Q000235:genetics* / Q000254:growth & development*; D002469:Cell Separation; D005434:Flow Cytometry; D008828:Microbiological Techniques; D008854:Microscopy, Electron; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013559:Symbiosis