title: Paper spray ionization of noncovalent protein complexes.
abstract: Paper spray (PS) ionization, an ambient ionization method, has previously been explored as a direct and fast method for mass spectrometric analysis of complex mixtures. It has been applied to the analysis of a wide variety of compounds, mostly small molecules. The work reported here extends the application of PS ionization to noncovalent protein complexes on an ion mobility tandem mass spectrometer. Similar mass spectra for protein complexes were obtained by PS ionization and nanoflow electrospray ionization (nESI), indicating that intact protein complexes can be preserved in PS ionization. In addition, collisional cross sections measured by ion mobility provide evidence that the protein assemblies may remain compact by PS ionization. With PS, it is possible to detect hemoglobin tetramer from a blood sample with minimal sample preparation. This is the first report to show that PS ionization is a promising ionization method for nonconvalent protein complexes.
authors.affiliation: Department of Chemistry and Biochemistry, The Ohio State University , 484 W. 12th Ave., Columbus, Ohio 43210, United States.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yue
authors.initials: Y
authors.lastname: Ju
authors.forename: Chengsi
authors.initials: C
authors.lastname: Huang
authors.forename: Vicki H
authors.initials: VH
authors.lastname: Wysocki
mesh_terms: D001798:Blood Proteins / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008958:Models, Molecular; D010209:Paper; D011487:Protein Conformation
chemical_list: D001798:Blood Proteins