title: [Comparative study on HPLC fingerprint of ordinary powder and ultrafine powder for Gardenia jasminoides f. longicarpa].
abstract: OBJECTIVE
  To establish an HPLC fingerprint of Gardenia jasminoides f. longicarpa and compare the differences between its ordinary powder and ultrafine powder.
  
  
  METHODS
  The analysis was carried out on a Kromasil C18 (250 mm x 4.6 mm, 5 microm) column with gradient elution of acetonitrile-0.4% phosphoric acid at the flow rate of 1.0 ml/min. The wavelength was 240 nm during 0 - 40 min and 440 nm during 40 - 80 min.
  
  
  RESULTS
  HPLC fingerprint of Gardenia jasminoides f. longicarpa was established, 23 common peaks were identified,and the similarity of 10 samples was greater than 0.9. Ultrafine grinding did not change the types and number of chemical compositions, but it obviously increased the content of main chemical compositions.
  
  
  CONCLUSIONS
  The HPLC fingerprint is accurate, reliable and repeatable, which can be used for quality control of Gardenia jasminoides f. longicarpa. Ultrafine grinding can stimulate the release of chemical components of Gardenia jasminoides f. longicarpa.
authors.affiliation: College of Pharmacy, Inner Mongolia Medical University, Hohhot 010059, China. hhhtnmyxyjih5511@sina.com
authors.forename: Ai-Hua
authors.initials: AH
authors.lastname: Ju
authors.affiliation: Inner Mongolia Institute for Food and Drug Control, Hohhot 010059, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhou
authors.affiliation: College of Pharmacy, Inner Mongolia Medical University, Hohhot 010059, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, Inner Mongolia Medical University, Hohhot 010059, China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Cai
authors.affiliation: College of Pharmacy, Inner Mongolia Medical University, Hohhot 010059, China.
authors.forename: Ping-Ping
authors.initials: PP
authors.lastname: Song
mesh_terms: D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D032062:Gardenia / Q000737:chemistry* / Q000254:growth & development; D010316:Particle Size; D011208:Powders; D011786:Quality Control; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis
chemical_list: D004365:Drugs, Chinese Herbal; D011208:Powders; D012503:Saponins