title: Aliidiomarina shirensis sp. nov., a halophilic bacterium isolated from Shira Lake in Khakasia, southern Siberia, and a proposal to transfer Idiomarina maris to the genus Aliidiomarina.
abstract: Strain AIS(T), an aerobic halophilic, Gram-reaction-negative, heterotrophic bacterium isolated from the water of Shira Lake in Khakasia, southern Siberia, was characterized using a polyphasic approach. Our analysis of the 16S rRNA gene sequences showed that 'Aliidiomarina haloalkalitolerans', 'Aliidiomarina sanyensis', Idiomarina maris and AIS(T) formed a distinct lineage. The sequence similarities between AIS(T) and the type strains of species of the genera Idiomarina and Aliidiomarina were 91.6-95.1 % and 94.0-96.9 %, respectively. The major isoprenoid quinone of AIS(T) was ubiquinone 8 (Q-8). Predominant cellular fatty acids were iso-C17 : 0, iso-C15 : 0 and summed feature 9. The genomic DNA G+C content was 45.8 mol%. It is concluded that AIS(T) represents a novel species of the genus Aliidiomarina, and the name Aliidiomarina shirensis sp. nov. is herein proposed for it. The type strain is AIS(T) ( = JCM 17761(T) = BCRC 80327(T)). Based on its fatty acid profile and our phylogenetic analysis, we propose that Idiomarina maris be transferred to the genus Aliidiomarina.
authors.affiliation: Biodiversity Research Center, Academia Sinica, Taipei, Taiwan, ROC.
authors.forename: Hsiu-Hui
authors.initials: HH
authors.lastname: Chiu
authors.affiliation: Siberian Federal University, Krasnoyarsk, Russia.|Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russia.
authors.forename: Denis Yu
authors.initials: DY
authors.lastname: Rogozin
authors.affiliation: Institute of Oceanography, National Taiwan University, PO Box 23-13, Taipei, Taiwan, ROC.
authors.forename: Ssu-Po
authors.initials: SP
authors.lastname: Huang
authors.affiliation: Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russia.
authors.forename: Andrei G
authors.initials: AG
authors.lastname: Degermendzhy
authors.affiliation: Institute of Oceanography, National Taiwan University, PO Box 23-13, Taipei, Taiwan, ROC.
authors.forename: Wung Yang
authors.initials: WY
authors.lastname: Shieh
authors.affiliation: Biodiversity Research Center, Academia Sinica, Taipei, Taiwan, ROC.
authors.forename: Sen-Lin
authors.initials: SL
authors.lastname: Tang
mesh_terms: D034241:Alteromonadaceae / Q000145:classification*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D060106:Lakes / Q000382:microbiology*; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012800:Siberia; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8