title: TFDP3 was expressed in coordination with E2F1 to inhibit E2F1-mediated apoptosis in prostate cancer.
abstract: TFDP3 has been previously identified as an inhibitor of E2F molecules. It has been shown to suppress E2F1-induced apoptosis dependent P53 and to play a potential role in carcinogenesis. However, whether it indeed helps cancer cells tolerate apoptosis stress in cancer tissues remains unknown. TFDP3 expression was assessed by RT-PCR, in situ hybridization and immunohistochemistry in normal human tissues, cancer tissues and prostate cancer tissues. The association between TFDP3 and E2F1 in prostate cancer development was analyzed in various stages. Apoptosis was evaluated with annexin-V and propidium iodide staining and flow-cytometry. The results show that, in 96 samples of normal human tissues, TFDP3 could be detected in the cerebrum, esophagus, stomach, small intestine, bronchus, breast, ovary, uterus, and skin, but seldom in the lung, muscles, prostate, and liver. In addition, TFDP3 was highly expressed in numerous cancer tissues, such as brain-keratinous, lung squamous cell carcinoma, testicular seminoma, cervical carcinoma, skin squamous cell carcinoma, gastric adenocarcinoma, liver cancer, and prostate cancer. Moreover, TFDP3 was positive in 23 (62.2%) of 37 prostate cancer samples regardless of stage. Furthermore, immunohistochemistry results show that TFDP3 was always expressed in coordination with E2F1 at equivalent expression levels in prostate cancer tissues, and was highly expressed particularly in samples of high stage. When E2F1 was extrogenously expressed in LNCap cells, TFDP3 could be induced, and the apoptosis induced by E2F1 was significantly decreased. It was demonstrated that TFDP3 was a broadly expressed protein corresponding to E2F1 in human tissues, and suggested that TFDP3 is involved in prostate cancer cell survival by suppressing apoptosis induced by E2F1.
authors.affiliation: Department of Clinical Laboratory, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yueyun
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Clinical Laboratory, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yijuan
authors.initials: Y
authors.lastname: Xin
authors.affiliation: Department of Clinical Laboratory, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.affiliation: Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Qiaohong
authors.initials: Q
authors.lastname: Yue
authors.affiliation: Department of Clinical Laboratory, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Fengjing
authors.initials: F
authors.lastname: Xiao
authors.affiliation: Department of Clinical Laboratory, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. Electronic address: haoxkg@fmmu.edu.cn.
authors.forename: Xiaoke
authors.initials: X
authors.lastname: Hao
mesh_terms: D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D050687:E2F1 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D012016:Reference Values; D050680:Transcription Factor DP1 / Q000235:genetics* / Q000378:metabolism*
chemical_list: D050687:E2F1 Transcription Factor; C493753:E2F1 protein, human; C516920:TFDP3 protein, human; D050680:Transcription Factor DP1
keywords: Apoptosis; CTAB; DAB; DIG; DMEM; Dulbecco's Modified Eagle's Medium; E2F transcription factor 1; E2F1; FACS; HRP; Horseradish Peroxidase; IHC; Prostate cancer; RT-PCR; TAE; TFDP3; Tris base, acetic acid and EDTA; diaminobenzidine; digoxigenin; flow cytometry; hexadecyltrimethylammonium bromide; immunohistochemistry; p16(INK4a)-cyclin D-retinoblastoma tumor suppressor; pRb; reverse transcription polymerase chain reaction; transcription factor Dp family, member 3