title: Comparative genomic hybridization in a case of melanoma that loses expression of S100, HMB45, Melan A and tyrosinase in metastasis.
abstract: We recently reported three cases of metastatic melanoma that does not express S100, HMB45, Melan A and Tyrosinase. A concurrent cutaneous scalp primary melanoma was identified later in one of the cases, which showed strong expression of these markers. The difference in immunophenotype between the primary melanoma and its metastasis in the parotid gland in this case raised the question of the biological significance of the expression of these markers and metastatic potential. To address this question, we utilized microarray comparative genomic hybridization (aCGH) to compare the cytogenetic features between the primary and metastatic melanoma. We observed chromosomal gains including 6p, entire chromosome 7, and 8q11.1-q24.3 in both primary and metastatic tumors. However, the metastatic lesion showed unique additional copy of chromosomal 7q, and loss of chromosome 9p24.3-q13 and chromosome 4, which included Melan A encoding gene region in 9p24.1. The above findings suggest the unique cytogenetic changes in the parotid lesion are most likely related to the metastatic behavior, as well as responsible for loss of multiple melanocytic marker expression in the metastatic melanoma for this case.
authors.affiliation: Department of Pathology, University of Oklahoma Health Sciences Center Oklahoma city, OK, USA.
authors.forename: Ruifeng
authors.initials: R
authors.lastname: Guo
authors.affiliation: Department of Pediatrics, Genetics Laboratory, University of Oklahoma Health Sciences Center Oklahoma city, OK, USA.
authors.forename: Xianfu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pathology, University of Oklahoma Health Sciences Center Oklahoma city, OK, USA.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Pathology, University of Oklahoma Health Sciences Center Oklahoma city, OK, USA.
authors.forename: Ellizabeth
authors.initials: E
authors.lastname: Gillies
authors.affiliation: Department of Pathology, University of Oklahoma Health Sciences Center Oklahoma city, OK, USA.
authors.forename: Kar-Ming
authors.initials: KM
authors.lastname: Fung
authors.affiliation: Department of Pediatrics, Genetics Laboratory, University of Oklahoma Health Sciences Center Oklahoma city, OK, USA.
authors.forename: Shibo
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Pathology, University of Oklahoma Health Sciences Center Oklahoma city, OK, USA.
authors.forename: Lewis A
authors.initials: LA
authors.lastname: Hassell
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D055028:Comparative Genomic Hybridization; D006801:Humans; D058965:MART-1 Antigen / Q000032:analysis / Q000096:biosynthesis; D008545:Melanoma / Q000235:genetics* / Q000378:metabolism / Q000556:secondary; D058950:Melanoma-Specific Antigens / Q000032:analysis / Q000096:biosynthesis; D014442:Monophenol Monooxygenase / Q000032:analysis / Q000096:biosynthesis; D009362:Neoplasm Metastasis / Q000235:genetics*; D009418:S100 Proteins / Q000032:analysis / Q000096:biosynthesis; D012878:Skin Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D058951:gp100 Melanoma Antigen
chemical_list: D014408:Biomarkers, Tumor; D058965:MART-1 Antigen; C548667:MLANA protein, human; D058950:Melanoma-Specific Antigens; C548637:PMEL protein, human; D009418:S100 Proteins; D058951:gp100 Melanoma Antigen; D014442:Monophenol Monooxygenase
keywords: Melanoma; comparative genomic hybridization; cytogenetics; metastasis