title: Serum metabolomics study of polycystic ovary syndrome based on liquid chromatography-mass spectrometry.
abstract: Polycystic ovary syndrome (PCOS) is a complex, heterogeneous disorder, which produces in 5-10% reproductive age women. In this study, a nontargeted metabolomics approach based on ultra high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry is used to investigate serum metabolic characteristics of PCOS. PCOS women and healthy control can be clustered into two distinct groups based on multivariate statistical analysis. Significant increase in the levels of unsaturated free fatty acids, fatty acid amides, sulfated steroids, glycated amino acid and the decrease in levels of lysophosphatidylcholines, lysophosphatidylethanolamines, etc., were found. These metabolites showed abnormalities of lipid- and androgen-metabolism, increase of stearoyl-CoA desaturase (SCD) activity and accumulation of advanced glycation end-products in PCOS patients. On the basis of the binary logistic regression model, free fatty acid (FFA) 18:1/FFA 18:0, FFA 20:3, dihydrotestosterone sulfate, glycated phenylalanine, and uridine were combined as a diagnostic biomarker. The area under the curve (AUC) of combinational biomarker was 0.839 in 131 discovery phase samples and 0.874 in 109 validation phase samples. The findings of our study offer a new insight to understand the pathogenesis mechanism, and the discriminating metabolites may provide a prospect for PCOS diagnosis.
authors.affiliation: Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences , Dalian 116023, China.
authors.forename: Xinjie
authors.initials: X
authors.lastname: Zhao
authors.forename: Fang
authors.initials: F
authors.lastname: Xu
authors.forename: Bing
authors.initials: B
authors.lastname: Qi
authors.forename: Songli
authors.initials: S
authors.lastname: Hao
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Lihong
authors.initials: L
authors.lastname: Zou
authors.forename: Caixia
authors.initials: C
authors.lastname: Lu
authors.forename: Guowang
authors.initials: G
authors.lastname: Xu
authors.forename: Lihui
authors.initials: L
authors.lastname: Hou
mesh_terms: D000328:Adult; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics; D011085:Polycystic Ovary Syndrome / Q000097:blood*; D055815:Young Adult