title: Activity of daily living is associated with circulating CD34+/KDR+ cells and granulocyte colony-stimulating factor levels in patients after myocardial infarction.
abstract: The study aimed to investigate whether the extent of activities of daily living (ADL) of patients after myocardial infarction affect numbers of circulating CD34(+)/KDR(+) and CD45(+)/CD34(+) cells, which are supposed to protect structural and functional endothelial integrity. In a cross-sectional study, 34 male coronary artery disease patients with a history of myocardial infarction were assessed for times spent per week for specific physical ADL, including basic activities (instrumental ADL), leisure time activities, and sport activities, using a validated questionnaire. Individual specific activity times were multiplied with respective specific metabolic equivalent scores to obtain levels of specific activities. Numbers of circulating CD34(+)/KDR(+) and CD45(+)/CD34(+) cells were analyzed by flow cytometry. Furthermore, the colony-forming capacity of CD34(+) cells and the level of granulocyte colony-stimulating factor (G-CSF) in serum were measured. Analysis revealed that the extent of total activities and basic activities, as well as total activity time, were positively correlated with numbers of circulating CD34(+)/KDR(+) cells (r = 0.60, 0.56, and 0.55, P < 0.05). Higher levels of total activity were also associated with increased colony-forming capacity of CD34(+) cells (r = 0.54, P < 0.05) and with higher systemic levels of G-CSF (r = 0.44, P < 0.05). These findings indicate that even ADL-related activities of coronary artery disease patients after myocardial infarction exert stimulating effects on CD34(+)/KDR(+) cell mobilization, potentially mediated by increased G-CSF levels. This, in turn, potentially contributes to the beneficial effects of exercise on the diseased cardiovascular system.
authors.affiliation: Department of Sports Medicine, Institute of Sports Sciences, Justus-Liebig-University, Giessen, Germany.
authors.forename: Karsten
authors.initials: K
authors.lastname: Krüger
authors.forename: Rainer
authors.initials: R
authors.lastname: Klocke
authors.forename: Julia
authors.initials: J
authors.lastname: Kloster
authors.forename: Sigrid
authors.initials: S
authors.lastname: Nikol
authors.forename: Johannes
authors.initials: J
authors.lastname: Waltenberger
authors.forename: Frank C
authors.initials: FC
authors.lastname: Mooren
mesh_terms: D000203:Activities of Daily Living; D000368:Aged; D018952:Antigens, CD34 / Q000378:metabolism / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism*; D006801:Humans; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009043:Motor Activity; D009203:Myocardial Infarction / Q000378:metabolism* / Q000503:physiopathology*; D009391:Nephelometry and Turbidimetry; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism / Q000502:physiology*
chemical_list: D018952:Antigens, CD34; D016179:Granulocyte Colony-Stimulating Factor; D040301:Vascular Endothelial Growth Factor Receptor-2; D017493:Leukocyte Common Antigens
keywords: coronary artery disease; endothelial function; endothelial progenitor cells; exercise; physical activity