title: Lysis of mammalian and Sf9 cells.
abstract: Use a French press to disrupt mammalian or Sf9 cells and generate a clarified lysate for subsequent use in protein purification.
authors.affiliation: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Kavran
authors.affiliation: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: dleahy@jhmi.edu.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Leahy
mesh_terms: D000818:Animals; D002021:Buffers; D002457:Cell Extracts / Q000737:chemistry; D002458:Cell Fractionation / Q000379:methods*; D006801:Humans; D061987:Sf9 Cells; D018411:Spodoptera
chemical_list: D002021:Buffers; D002457:Cell Extracts
keywords: Cell lysate clarification; Eukaryotic cells; Mammalian cells; Resuspend cells in lysis buffer; Sf9 cells