title: Continuous enrichment of low-abundance cell samples using standing surface acoustic waves (SSAW).
abstract: Cell enrichment is a powerful tool in a variety of cellular studies, especially in applications with low-abundance cell types. In this work, we developed a standing surface acoustic wave (SSAW) based microfluidic device for non-contact, continuous cell enrichment. With a pair of parallel interdigital transducers (IDT) deposited on a piezoelectric substrate, a one-dimensional SSAW field was established along disposable micro-tubing channels, generating numerous pressure nodes (and thus numerous cell-enrichment regions). Our method is able to concentrate highly diluted blood cells by more than 100 fold with a recovery efficiency of up to 99%. Such highly effective cell enrichment was achieved without using sheath flow. The SSAW-based technique presented here is simple, bio-compatible, label-free, and sheath-flow-free. With these advantages, it could be valuable for many biomedical applications.
authors.affiliation: Department of Engineering Science and Mechanics, The Pennsylvania, State University, University Park, PA 16802, USA. junhuang@psu.edu.
authors.forename: Yuchao
authors.initials: Y
authors.lastname: Chen
authors.forename: Sixing
authors.initials: S
authors.lastname: Li
authors.forename: Yeyi
authors.initials: Y
authors.lastname: Gu
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Ding
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: J Philip
authors.initials: JP
authors.lastname: McCoy
authors.forename: Stewart J
authors.initials: SJ
authors.lastname: Levine
authors.forename: Tony Jun
authors.initials: TJ
authors.lastname: Huang
mesh_terms: D001773:Blood Cells / Q000166:cytology; D002470:Cell Survival; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D010316:Particle Size; D013016:Sound
chemical_list: D005456:Fluorescent Dyes