title: Study of expression of TLR2, TLR4 and transckription factor NF-kB structures of galt of rats in the conditions of the chronic social stress and modulation of structure of intestinal microflora.
abstract: The present study was conducted to investigate of the influence of chronic social stress (CSS) and modulation of the composition of intestinal microflora on the distribution of TLR2+-, TLR4+- and Nf-kB+-cells in the GALT of ileum of the rats. Researchers have been conducted on 84 rats (female) of Wistar line, which were divided on 7 experimental groups: control rats (group 1); rats, which were modeled CSS1 by means of three weeks social isolation and prolong psychoemotional influence (group2); rats, which having CSS 2 modeling by means of keeping animals in over populated cages with every day change of grouping (group 3); rats with CSS1 and CSS2, which were made the modeling of intestinal microflora by means of administrations of aminoglycosed antibiotic kanamycin (group 4 and 5, accordingly); rats with CSS1 and CSS2, which were made the modeling of intestinal microflora by means of everyday administrations of lactobacterine (groups 6 and 7, accordingly). Structure of population of TLR2+-, TLR4+- and Nf-kB+-cells has been studied by the analysis of serial histological sections using the method of direct and indirect immunofluorescense with monoclonal antibodies to TLR2, TLR4 and Nf-kB. CSS development is accompanied with increase in total lymphocytes expressing TLR2 and 4 type GALT rats with the most pronounced in LFV (TLR2+-lymphocytes) and PP LFs (TLR4+-cells) led to an increase in the number of Nf-kB+- cells: in LFV a 1.8-2 fold (p<0.05) in PP at the sub - 52-91% (p<0.05) in PP LFs - for 89-92% (p<0.05), and it is also influenced on the density of TLR2, TLR4, and the concentration of Nf-kB in immunopositive cells. AB and PB injections were accompanied by a decrease in the number of studied cells, so in the separate zone GALT is increased to their number, changing the density of immune system receptors.
authors.affiliation: Zaporozhye State Medical University, Department of Microbiology, Virusology and Immunology, Ukraine.
authors.forename: I
authors.initials: I
authors.lastname: Topol
authors.affiliation: Zaporozhye State Medical University, Department of Microbiology, Virusology and Immunology, Ukraine;
authors.forename: A
authors.initials: A
authors.lastname: Kamyshny
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000382:microbiology; D064307:Microbiota; D016328:NF-kappa B / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D013315:Stress, Psychological / Q000235:genetics* / Q000473:pathology; D051195:Toll-Like Receptor 2 / Q000096:biosynthesis*; D051197:Toll-Like Receptor 4 / Q000096:biosynthesis*
chemical_list: D016328:NF-kappa B; C495343:Tlr2 protein, rat; C495347:Tlr4 protein, rat; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4