title: Structural characterization of the degradation products of a minor natural sweet diterpene glycoside Rebaudioside M under acidic conditions.
abstract: Degradation of rebaudioside M, a minor sweet component of Stevia rebaudiana Bertoni, under conditions that simulated extreme pH and temperature conditions has been studied. Thus, rebaudioside M was treated with 0.1 M phosphoric acid solution (pH 2.0) and 80 °C temperature for 24 h. Experimental results indicated that rebaudioside M under low pH and higher temperature yielded three minor degradation compounds, whose structural characterization was performed on the basis of 1D (1H-, 13C-) &amp; 2D (COSY, HSQC, HMBC) NMR, HRMS, MS/MS spectral data as well as enzymatic and acid hydrolysis studies.
authors.affiliation: Organic Chemistry Department, The Coca-Cola Company, Global Research and Development, One Coca-Cola Plaza, Atlanta, GA 30313, USA. iprakash@coca-cola.com.
authors.forename: Indra
authors.initials: I
authors.lastname: Prakash
authors.affiliation: Organic Chemistry Department, The Coca-Cola Company, Global Research and Development, One Coca-Cola Plaza, Atlanta, GA 30313, USA. saipc@bluecal-ingredients.com.
authors.forename: Venkata Sai Prakash
authors.initials: VS
authors.lastname: Chaturvedula
authors.affiliation: Organic Chemistry Department, The Coca-Cola Company, Global Research and Development, One Coca-Cola Plaza, Atlanta, GA 30313, USA. avetik@purecircle.com.
authors.forename: Avetik
authors.initials: A
authors.lastname: Markosyan
mesh_terms: D000143:Acids / Q000737:chemistry; D045786:Diterpenes, Kaurane / Q000737:chemistry* / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D013549:Sweetening Agents / Q000737:chemistry*; D013696:Temperature; D014312:Trisaccharides / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000143:Acids; D045786:Diterpenes, Kaurane; D013549:Sweetening Agents; D014312:Trisaccharides; C000590692:rebaudioside M