title: Needle-free immunization using a solid-in-oil nanodispersion enhanced by a skin-permeable oligoarginine peptide.
abstract: The objective of transcutaneous immunization is efficient vaccination using the skin's immune system. Although a less invasive administration procedure is involved, the effective delivery of antigen using this modality remains a problem. Here, we demonstrate the use of a solid-in-oil (S/O) nanodispersion system for the transcutaneous immunization of male ddY mice with ovalbumin (OVA) antigen. The S/O nanoparticles consisted of OVA and hydrophobic surfactant molecules and were dispersed in oil; enhanced induction of antigen-specific antibody was observed after the addition of polyarginine (R6) into the same S/O nanoparticle containing OVA. The improved S/O nanodispersion system induced a comparable level of OVA-specific antibody to that induced by subcutaneous injection of OVA at the same dose, advocating the potential application of the S/O system as a needle-free and easy-to-use immunization system.
authors.forename: Momoko
authors.initials: M
authors.lastname: Kitaoka
authors.forename: Kana
authors.initials: K
authors.lastname: Imamura
authors.forename: Yuya
authors.initials: Y
authors.lastname: Hirakawa
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Tahara
authors.forename: Noriho
authors.initials: N
authors.lastname: Kamiya
authors.forename: Masahiro
authors.initials: M
authors.lastname: Goto
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000941:Antigens / Q000737:chemistry* / Q000276:immunology; D004337:Drug Carriers / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D007114:Immunization / Q000379:methods; D008297:Male; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D009339:Needles; D009821:Oils / Q000737:chemistry*; D010047:Ovalbumin / Q000737:chemistry / Q000276:immunology; D010455:Peptides / Q000737:chemistry* / Q000276:immunology; D012867:Skin / Q000276:immunology* / Q000378:metabolism; D013501:Surface-Active Agents / Q000138:chemical synthesis; D014611:Vaccination / Q000379:methods
chemical_list: D000906:Antibodies; D000941:Antigens; D004337:Drug Carriers; D009821:Oils; D010455:Peptides; D013501:Surface-Active Agents; C015462:polyarginine; D010047:Ovalbumin