title: Mating type gene (MAT1-2) of Trichophyton verrucosum.
abstract: Trichophyton verrucosum is a zoophilic dermatophyte species that is the most frequent etiologic agent of bovine dermatophytosis throughout the world. Since no teleomorph of T. verrucosum has been found, polymerase chain reaction (PCR) analysis on the genome of T. verrucosum isolated from the Czech Republic and Japan was performed to confirm the presence of a mating type locus in the genome of the fungus and to clarify its classification and ecological characteristics. The mating type gene (MAT1-2) allele was detected by PCR analysis in all 22 isolates (four isolates from the Czech Republic and 18 isolates from Japan). The nucleotide sequence of the region exhibited 99-100 % identity among all isolates, including the reference strain of T. verrucosum. Phylogenetic analysis revealed that the sequences of the internal transcribed spacer region at the MAT1-2 locus clustered together in the isolates examined, forming a branch distinct from that of the other dermatophyte species. These results suggest that T. verrucosum is a clonal offshoot that has drifted away from Arthroderma benhamiae.
authors.affiliation: Department of Pathobiology, Nihon University School of Veterinary Medicine, 1866 Kameino, Fujisawa, Kanagawa, 252-0880, Japan, kanou.rui@nihon-u.ac.jp.
authors.forename: Rui
authors.initials: R
authors.lastname: Kano
authors.forename: Erina
authors.initials: E
authors.lastname: Yoshida
authors.forename: Takashi
authors.initials: T
authors.lastname: Yaguchi
authors.forename: Vit
authors.initials: V
authors.lastname: Hubka
authors.forename: Kazushi
authors.initials: K
authors.lastname: Anzawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Mochizuki
authors.forename: Atsuhiko
authors.initials: A
authors.lastname: Hasegawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kamata
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D004271:DNA, Fungal / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D049770:Genes, Mating Type, Fungal / Q000235:genetics*; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014005:Tinea / Q000382:microbiology / Q000662:veterinary*; D014249:Trichophyton / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins