title: Single nucleotide polymorphisms in the D-loop region of mitochondrial DNA are associated with epithelial ovarian cancer prognosis.
abstract: OBJECTIVE
  The single nucleotide polymorphisms (SNPs) in the displacement loop (D-loop) of mitochondrial DNA (mtDNA) is found in many types of cancers. A lot of reports show the SNPs of D-loop may be related to cancers prognosis. This study investigated whether the SNPs were associated with the outcome of epithelial ovarian cancer (EOC).
  
  
  METHODS
  The D-loop region of mtDNA samples from blood were obtained from 89 patients with primary EOC, who received surgery. The 3 year survival curves were calculated by Kaplan-Meier method and compared by the log-rank test. Multivariate analysis was performed with Cox proportional hazards model.
  
  
  RESULTS
  The SNP sites of nucleotides 309 C/T, 324 C/G and 446 C/A were identified for prediction of post-operational survival. In an overall multivariate analysis, the alleles 309 and 324 were identified as independent predictors of EOC outcome.
  
  
  CONCLUSIONS
  The results indicated that the SNPs in D-loop are associated with the clinical outcome of patients with EOC. The analysis of SNPs in D-loop region may help identify the patient genotype to predict prognosis of the EOC patients.
authors.affiliation: a Department of Gynaecology and Obstetrics , The Second Hospital of Hebei Medical University , Shijiazhuang , P.R. China.
authors.forename: Desheng
authors.initials: D
authors.lastname: Kong
authors.affiliation: a Department of Gynaecology and Obstetrics , The Second Hospital of Hebei Medical University , Shijiazhuang , P.R. China.
authors.forename: Shuhong
authors.initials: S
authors.lastname: Shi
authors.affiliation: a Department of Gynaecology and Obstetrics , The Second Hospital of Hebei Medical University , Shijiazhuang , P.R. China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000077216:Carcinoma, Ovarian Epithelial; D002648:Child; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D005260:Female; D006801:Humans; D008875:Middle Aged; D009375:Neoplasms, Glandular and Epithelial / Q000175:diagnosis* / Q000235:genetics* / Q000401:mortality / Q000601:surgery; D009690:Nucleic Acid Conformation; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000235:genetics* / Q000401:mortality / Q000601:surgery; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D016019:Survival Analysis; D055815:Young Adult
chemical_list: D004272:DNA, Mitochondrial
keywords: Cancer; D-loop; mitochondria; outcome; ovarian