title: The Primary Research on the Gut Microbes in KKAy Mice.
abstract: In the first part of this paper, gut microbial difference of two genotypes mice was researched. The gut microbial community of type 2 diabetes animal model KKAy mice and normal C57BL/6J mice had clear distinctions in DGGE (denaturing gradient gel electrophoresis) profiles. The pairwise similarity coefficient (C s ) was only 26-44 % between KKAy and C57BL/6J, but C s was 82-100 % among same genotypes mice. Thirteen dominant bands were cloned from DGGE profiles to exhibit difference on gut microbial structure further. In the second part of this paper, the influence of hypoglycemic drug Pioglitazone on the gut microbes in KKAy mice was researched by gut microbial diversity analysis and principal component analysis (PCA). The results showed that Pioglitazone reduced the gut microbial diversity slightly and changed gut microbial structure of KKAy mice to that of normal C57BL/6J mice.
authors.affiliation: Institute of Biotechnology, Shanxi University, 92 Wucheng Road, Taiyuan, 030006 China.
authors.forename: Yanqin
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Biotechnology, Shanxi University, 92 Wucheng Road, Taiyuan, 030006 China.
authors.forename: Liang
authors.initials: L
authors.lastname: Han
authors.affiliation: Institute of Biotechnology, Shanxi University, 92 Wucheng Road, Taiyuan, 030006 China.
authors.forename: Ming
authors.initials: M
authors.lastname: Xu
authors.affiliation: Shanxi Hospital of Traditional Chinese Medicine, Taiyuan, 030012 China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Guo
authors.affiliation: Shanxi Hospital of Traditional Chinese Medicine, Taiyuan, 030012 China.
authors.forename: Mali
authors.initials: M
authors.lastname: Feng
authors.affiliation: Shanxi Hospital of Traditional Chinese Medicine, Taiyuan, 030012 China.
authors.forename: Xiaojiong
authors.initials: X
authors.lastname: Wang
keywords: Genotypes; Gut microbes; KKAy mice; Pioglitazone; Type 2 diabetes