title: Biomarkers of coagulation, fibrinolysis, endothelial function, and inflammation in arterialized venous blood.
abstract: Effects of venous blood arterialization on cardiovascular risk markers are still unknown. We evaluated biomarkers of inflammation, coagulation, fibrinolysis, and endothelial function in arterialized compared with regular venous blood. Cubital venipunctures were obtained from 10 healthy volunteers. Arterialization was generated by 10 min heating of the contralateral hand. Concentrations of albumin, C-reactive protein (CRP), tissue-type plasminogen activator (t-PA), plasminogen activator inhibitor type 1 (PAI-1), and von Willebrand factor (vWF) were measured by validated assays. Concentrations of albumin, CRP, and vWF were significantly lower in arterialized than in venous blood (albumin: 43.8 g/l and 44.8 g/l, P = 0.02). Differences in CRP and vWF became insignificant after adjusting for albumin. The endogenous thrombin potential (ETP) was significantly higher in arterialized than in venous blood (1929 nmol/l*min vs. 1872 nmol/l*min, P = 0.02). Addition of the FXIIa inhibitor Corn Trypsin Inhibitor (CTI) prior to the thrombin generation test eliminated the ETP difference suggesting that hand heating activates the FXII-dependent coagulation pathway.
authors.affiliation: aDepartment of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen bUnit for Thrombosis Research, Institute of Public Health, University of Southern Denmark and Department of Clinical Biochemistry, Hospital of South West Denmark, Esbjerg, Denmark.
authors.forename: Anne S
authors.initials: AS
authors.lastname: Gram
authors.forename: Jane
authors.initials: J
authors.lastname: Skov
authors.lastname: Thorkil Ploug
authors.forename: Johannes J
authors.initials: JJ
authors.lastname: Sidelmann
authors.forename: Bente M
authors.initials: BM
authors.lastname: Stallknecht
authors.forename: Else-M
authors.initials: EM
authors.lastname: Bladbjerg
mesh_terms: D015415:Biomarkers / Q000097:blood; D001777:Blood Coagulation / Q000502:physiology*; D002318:Cardiovascular Diseases / Q000097:blood*; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology*; D005342:Fibrinolysis / Q000502:physiology*; D064368:Healthy Volunteers; D006801:Humans; D007249:Inflammation / Q000097:blood*; D012307:Risk Factors
chemical_list: D015415:Biomarkers