title: Antigenic mapping of the hemagglutinin of an H9N2 avian influenza virus reveals novel critical amino acid positions in antigenic sites.
abstract: H9N2 influenza virus is undergoing extensive genetic and antigenic evolution, warranting detailed antigenic mapping of its hemagglutinin (HA). Through examining antibody escape mutants of an Asian avian H9N2 virus, we identified 9 critical amino acid positions in H9 antigenic sites. Five of these positions, 164, 167, 168, 196, and 207, have not been reported previously and, thus, represent novel molecular markers for monitoring the antigenic change of H9N2 virus.
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Yangzhou, China, and Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Wan
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Ye
authors.forename: Liangliang
authors.initials: L
authors.lastname: Xu
authors.forename: Hongxia
authors.initials: H
authors.lastname: Shao
authors.forename: Wenjie
authors.initials: W
authors.lastname: Jin
authors.forename: Kun
authors.initials: K
authors.lastname: Qian
authors.forename: Hongquan
authors.initials: H
authors.lastname: Wan
authors.forename: Aijian
authors.initials: A
authors.lastname: Qin
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D004252:DNA Mutational Analysis; D018604:Epitope Mapping; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology*; D053127:Influenza A Virus, H9N2 Subtype / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000235:genetics / Q000276:immunology; D017422:Sequence Analysis, DNA
chemical_list: D000956:Antigens, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D050505:Mutant Proteins