title: Stabilization of steroid 11-hydroxylation activity ofCunninghamella elegans protoplasts in organic osmotic stabilizers.
abstract: Protoplasts ofCunninghamella elegans, showing 11α-, and 11β-hydroxylating ability of Substance S, preserved high transformation activity when dispersed in glucose-enriched, organic osmotic stabilizers. A joint action of polyoxins and 2-deoxy-D-glucose was necessary to prevent regeneration of the cell wall in long-lasting experiments. Stabilized and active, dispersed protoplasts may be an alternative research model for studying the function of the cell wall and intracellular metabolic pool constituents in steroid hydroxylation.
authors.affiliation: Institute of Microbiology, University of Lódź, Banacha 12/16, 90-237, Lódź, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Dtugoński
authors.forename: K
authors.initials: K
authors.lastname: Bartnicka
authors.forename: V
authors.initials: V
authors.lastname: Chojecka
authors.forename: L
authors.initials: L
authors.lastname: Sedlaczek