title: Effects of different target sites on antisense RNA-mediated regulation of gene expression.
abstract: Antisense RNA is a type of noncoding RNA (ncRNA) that binds to complementary mRNA sequences and induces gene repression by inhibiting translation or degrading mRNA. Recently, several small ncRNAs (sRNAs) have been identified in Escherichia coli that act as antisense RNA mainly via base pairing with mRNA. The base pairing predominantly leads to gene repression, and in some cases, gene activation. In the current study, we examined how the location of target sites affects sRNA-mediated gene regulation. An efficient antisense RNA expression system was developed, and the effects of antisense RNAs on various target sites in a model mRNA were examined. The target sites of antisense RNAs suppressing gene expression were identified, not only in the translation initiation region (TIR) of mRNA, but also at the junction between the coding region and 3' untranslated region. Surprisingly, an antisense RNA recognizing the upstream region of TIR enhanced gene expression through increasing mRNA stability.
authors.affiliation: Department of Chemistry, KAIST, Daejeon 305-701, Korea.
authors.forename: Hongmarn
authors.initials: H
authors.lastname: Park
authors.affiliation: Korea Science Academy of KAIST, Busan 614-822, Korea.
authors.forename: Yeongseong
authors.initials: Y
authors.lastname: Yoon
authors.affiliation: Department of Chemistry, KAIST, Daejeon 305-701, Korea.
authors.forename: Shinae
authors.initials: S
authors.lastname: Suk
authors.affiliation: Department of Chemistry, KAIST, Daejeon 305-701, Korea.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Department of Chemistry, KAIST, Daejeon 305-701, Korea.
authors.forename: Younghoon
authors.initials: Y
authors.lastname: Lee
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D010442:Peptide Chain Initiation, Translational; D020871:RNA Stability; D016372:RNA, Antisense / Q000737:chemistry / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D016372:RNA, Antisense; D012333:RNA, Messenger