title: Aptamer-based portable biosensor for platelet-derived growth factor-BB (PDGF-BB) with personal glucose meter readout.
abstract: A novel portable biosensor for sensitive and selective detection of platelet-derived growth factor-BB (PDGF-BB) had been developed based on the specific recognition between aptamer and protein using a personal glucose meter (PGM) as readout. In the presence of PDGF-BB, the primary aptamer of PDGF-BB which is bound to the surface of streptavidin magnespheres paramagnetic particles (SA-PMPs) reacts quantitatively with invertase-functionalized secondary aptamer of PDGF-BB to form a stable complex, resulting in the attachment of invertase on the SA-PMPs. Subsequently, the invertase catalyzes the hydrolysis of sucrose to produce a large amount of glucose and quantitative readout by the PGM. The enhanced signal of the PGM has a relationship with the concentration of PDGF-BB in the range of 1.0 × 10(-14) M~3.16 × 10(-12) M, and the detection limit is 2.9 fM. The proposed portable biosensor had been successfully applied to assay the PDGF-BB in saliva samples.
authors.affiliation: MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Ma
authors.affiliation: MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China.
authors.forename: Zhonghui
authors.initials: Z
authors.lastname: Chen
authors.affiliation: MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Chemistry and Environmental Science, Minnan Normal University, Zhangzhou, Fujian 363000, China. Electronic address: weng_wen@126.com.
authors.forename: Wen
authors.initials: W
authors.lastname: Weng
authors.affiliation: MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China.
authors.forename: Ou
authors.initials: O
authors.lastname: Zheng
authors.affiliation: MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China. Electronic address: zylin@fzu.edu.cn.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Lin
authors.affiliation: MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China.
authors.forename: Longhua
authors.initials: L
authors.lastname: Guo
authors.affiliation: MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Qiu
authors.affiliation: MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China.
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics*; D001483:Base Sequence; D000077214:Becaplermin; D015374:Biosensing Techniques / Q000295:instrumentation*; D015190:Blood Glucose Self-Monitoring / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D008904:Miniaturization; D008969:Molecular Sequence Data; D019095:Point-of-Care Systems; D020574:Proto-Oncogene Proteins c-sis / Q000032:analysis*; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D052157:Aptamers, Nucleotide; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin
keywords: Aptamer; Biosensor; Personal glucose meters; Platelet-derived growth factor B