title: Diagnostic value of PCR in genitourinary tuberculosis.
abstract: Genitourinary tuberculosis is a disease of the genitourinary system which includes the entire urinary tract and reproductive system. Genital tuberculosis is an important cause of female infertility, especially in developing nations like India. In the present study, a total of 257 clinical specimens comprising of endometrial biopsy (109), endometrial curetting (42), menstrual blood (8), semen (17), placenta (11) and urine (70) were collected from patients and subjected for PCR, Culture and AFB detection. The endometrial biopsy, endometrial curetting, menstrual blood, semen, placenta, urine showed 30.2, 45.2,12.5, 5.8, 27.2, 31.4 %, positivity rate for tuberculosis by PCR, 7.3, 9.5, 25.0, 0, 9, 8.5 % by culture and 1.8, 2.3, 0, 0, 0, 2.8 % respectively by AFB smear. Being a novel, rapid technique, PCR is the method of choice for rapid diagnosis and management of genitourinary tuberculosis shared with the other concerned tests. This study reveals that genital tuberculosis can occur in any age group, however, the majority of patients were from reproductive age (nearly 75 % of them were from 20-45 years of age) group.
authors.affiliation: Molecular Research Laboratory, Department of Biochemistry, Shri Guru Ram Rai Institute of Medical & Health Sciences (SGRRIM&HS), Patel Nagar, Dehradun, 248001 Uttarakhand India.
authors.forename: Narotam
authors.initials: N
authors.lastname: Sharma
authors.affiliation: Department of Bioscience and Biotechnology, Banasthali University, Banasthali, 304022 Rajasthan India.
authors.forename: Veena
authors.initials: V
authors.lastname: Sharma
authors.affiliation: Department of Bioscience and Biotechnology, Banasthali University, Banasthali, 304022 Rajasthan India.
authors.forename: Prem Raj
authors.initials: PR
authors.lastname: Singh
authors.affiliation: Molecular Research Laboratory, Department of Biochemistry, Shri Guru Ram Rai Institute of Medical & Health Sciences (SGRRIM&HS), Patel Nagar, Dehradun, 248001 Uttarakhand India.
authors.forename: Shivani
authors.initials: S
authors.lastname: Sailwal
authors.affiliation: Molecular Research Laboratory, Department of Biochemistry, Shri Guru Ram Rai Institute of Medical & Health Sciences (SGRRIM&HS), Patel Nagar, Dehradun, 248001 Uttarakhand India.
authors.forename: Rajeev S
authors.initials: RS
authors.lastname: Kushwaha
authors.affiliation: Molecular Research Laboratory, Department of Biochemistry, Shri Guru Ram Rai Institute of Medical & Health Sciences (SGRRIM&HS), Patel Nagar, Dehradun, 248001 Uttarakhand India.
authors.forename: Rajesh K
authors.initials: RK
authors.lastname: Singh
authors.affiliation: Molecular Research Laboratory, Department of Biochemistry, Shri Guru Ram Rai Institute of Medical & Health Sciences (SGRRIM&HS), Patel Nagar, Dehradun, 248001 Uttarakhand India.
authors.forename: Satish C
authors.initials: SC
authors.lastname: Nautiyal
authors.affiliation: Molecular Research Laboratory, Department of Biochemistry, Shri Guru Ram Rai Institute of Medical & Health Sciences (SGRRIM&HS), Patel Nagar, Dehradun, 248001 Uttarakhand India ; Department of Community Medicine, Shri Guru Ram Rai Institute of Medical & Health Sciences (SGRRIM&HS), Patel Nagar, Dehradun, 248001 Uttarakhand India.
authors.forename: Pankaj
authors.initials: P
authors.lastname: Mishra
authors.affiliation: Molecular Research Laboratory, Department of Biochemistry, Shri Guru Ram Rai Institute of Medical & Health Sciences (SGRRIM&HS), Patel Nagar, Dehradun, 248001 Uttarakhand India.
authors.forename: Tariq
authors.initials: T
authors.lastname: Masood
authors.affiliation: Molecular Research Laboratory, Department of Biochemistry, Shri Guru Ram Rai Institute of Medical & Health Sciences (SGRRIM&HS), Patel Nagar, Dehradun, 248001 Uttarakhand India.
authors.forename: R K
authors.initials: RK
authors.lastname: Singh
keywords: Bactec 460; Genitourinary tuberculosis; MPB64; Polymerase chain reaction