title: The Arabidopsis chloroplast protein S-RBP11 is involved in oxidative and salt stress responses.
abstract: S-RBP11, a chloroplast protein, which was isolated using activation tagging system, is shown to be the first Arabidopsis small RNA-binding group protein involved in oxidative and salt stress responses. Activation tagging is one of the most powerful tools in reverse genetics. In this study, we isolated S-RBP11, encoding a small RNA-binding protein in Arabidopsis, by salt-resistant activation tagging line screen and then characterized its function in the abiotic stress response. The isolated activation tagging line of S-RBP11 as well as transgenic plants overexpressing S-RBP11 showed increased tolerance to salt and MV stresses compared to WT plants, whereas s-rbp11 mutants were more sensitive to salt stresses. Transcription of S-RBP11 was elevated upon MV treatment but not NaCl or cold treatment. Interestingly, S-RBP11 protein was localized in the chloroplast and the N-terminal 34 amino acid region of S-RBP11 was necessary for its chloroplast targeting. Our results suggest that S-RBP11 is a chloroplast protein involved in the responses to salt and oxidative stresses.
authors.affiliation: Department of Molecular Biology, Pusan National University, Busan, 609-735, Korea.
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Lee
authors.forename: Hye-Yeon
authors.initials: HY
authors.lastname: Seok
authors.forename: Vaishali N
authors.initials: VN
authors.lastname: Tarte
authors.forename: Dong-Hyuk
authors.initials: DH
authors.lastname: Woo
authors.forename: Dihn Huan
authors.initials: DH
authors.lastname: Le
authors.forename: Eun-Hye
authors.initials: EH
authors.lastname: Lee
authors.forename: Yong-Hwan
authors.initials: YH
authors.lastname: Moon
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D060365:Chloroplast Proteins / Q000235:genetics* / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D018384:Oxidative Stress; D010641:Phenotype; D030821:Plants, Genetically Modified; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D055049:Salt Tolerance; D036226:Seedlings / Q000166:cytology / Q000235:genetics / Q000502:physiology; D016415:Sequence Alignment; D013312:Stress, Physiological
chemical_list: D029681:Arabidopsis Proteins; D060365:Chloroplast Proteins; D016601:RNA-Binding Proteins; C000595284:S-RBP11 protein, Arabidopsis