title: Elucidating hydroxylation and methylation steps tailoring piericidin A1 biosynthesis.
abstract: The piericidin A1 (1) gene cluster was identified from the deep-sea derived Streptomyces sp. SCSIO 03032. Our in vivo and in vitro experiments verified PieE as a 4'-hydroxylase and PieB2 as a 4'-O-methyltransferase, allowing the elucidation of the post-PKS modification steps involved in 1 biosynthesis. In addition, the shunt metabolite piericidin E1 (7) was identified as a novel analogue featuring a C-2/C-3 epoxy ring.
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences , 164 West Xingang Road, Guangzhou 510301, China.
authors.forename: Yaolong
authors.initials: Y
authors.lastname: Chen
authors.forename: Wenjun
authors.initials: W
authors.lastname: Zhang
authors.forename: Yiguang
authors.initials: Y
authors.lastname: Zhu
authors.forename: Qingbo
authors.initials: Q
authors.lastname: Zhang
authors.forename: Xinpeng
authors.initials: X
authors.lastname: Tian
authors.forename: Si
authors.initials: S
authors.lastname: Zhang
authors.forename: Changsheng
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000378:metabolism; D006900:Hydroxylation; D008745:Methylation; D008780:Methyltransferases / Q000737:chemistry* / Q000378:metabolism; D015394:Molecular Structure; D005810:Multigene Family; D011725:Pyridines / Q000138:chemical synthesis* / Q000378:metabolism; D013302:Streptomyces / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D011725:Pyridines; C100213:piericidin A; D008780:Methyltransferases