title: Bovine papillomavirus E2 and E5 gene expression in sperm cells of healthy bulls.
abstract: Papillomaviruses are found in epithelial lesions and are linked to different carcinogenic processes in humans and other animals. Although BPV has been characterized as epitheliotropic, the presence of viral DNA has been detected in other tissues and fluids, such as fresh semen. The aim of this study was to evaluate the presence and expression of BPV in sperm cells of bulls (Bos taurus) asymptomatic for papillomatosis. A PCR assay was carried out with specific primers to test BPV2 in 26 semen samples. The presence of BPV transcripts was assessed by RT-PCR to E2 and E5 genes. BPV2 DNA was detected in nine out of 26 samples and the expression of E2 and E5 were detected in five out of nine BPV positive samples. This is the first record of BPV2 expression in bull sperm cells.
authors.affiliation: Laboratório de Estudos Moleculares e Terapia Experimental, Departamento de Genética, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, PE Brazil.
authors.forename: M A R
authors.initials: MA
authors.lastname: Silva
authors.affiliation: Laboratório de Andrologia, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Recife, PE Brazil.
authors.forename: E C B
authors.initials: EC
authors.lastname: Silva
authors.affiliation: Laboratório de Estudos Moleculares e Terapia Experimental, Departamento de Genética, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, PE Brazil.
authors.forename: A P A D
authors.initials: AP
authors.lastname: Gurgel
authors.affiliation: Laboratório de Estudos Moleculares e Terapia Experimental, Departamento de Genética, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, PE Brazil.
authors.forename: K C G
authors.initials: KC
authors.lastname: Nascimento
authors.affiliation: Laboratório de Estudos Moleculares e Terapia Experimental, Departamento de Genética, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, PE Brazil.
authors.forename: A C
authors.initials: AC
authors.lastname: Freitas
keywords: Bovine papillomavirus; Semen; Viral gene expression