title: Rapid purification of fructose-1,6-bisphosphate aldolase from spinach chloroplasts.
abstract: A rapid procedure for the purification of fructose-1,6-bisphosphate aldolase from spinach chloroplasts is presented which involves two steps; precipitation of bulk protein with polyethylene glycol and partitioning of remaining soluble protein in aqueous two-phase systems. A 94% pure preparation is obtained within 6h with a yield of 19%. A marked difference in the partition behaviour of the aldolase activity from whole leaf tissue suggested that the procedure is less efficient when leaf extract is used as starting material.
authors.affiliation: Department of Biochemistry, University of Lund, Box 124, S-221 00, LUND, Sweden.
authors.forename: L O
authors.initials: LO
authors.lastname: Persson