title: Bone tissue engineering by using a combination of polymer/Bioglass composites with human adipose-derived stem cells.
abstract: Translational research in bone tissue engineering is essential for "bench to bedside" patient benefit. However, the ideal combination of stem cells and biomaterial scaffolds for bone repair/regeneration is still unclear. The aim of this study is to investigate the osteogenic capacity of a combination of poly(DL-lactic acid) (PDLLA) porous foams containing 5 wt% and 40 wt% of Bioglass particles with human adipose-derived stem cells (ADSCs) in vitro and in vivo. Live/dead fluorescent markers, confocal microscopy and scanning electron microscopy showed that PDLLA/Bioglass porous scaffolds supported ADSC attachment, growth and osteogenic differentiation, as confirmed by enhanced alkaline phosphatase (ALP) activity. Higher Bioglass content of the PDLLA foams increased ALP activity compared with the PDLLA only group. Extracellular matrix deposition after 8 weeks in the in vitro cultures was evident by Alcian blue/Sirius red staining. In vivo bone formation was assessed by using scaffold/ADSC constructs in diffusion chambers transplanted intraperitoneally into nude mice and recovered after 8 weeks. Histological and immunohistochemical assays indicated significant new bone formation in the 40 wt% and 5 wt% Bioglass constructs compared with the PDLLA only group. Thus, the combination of a well-developed biodegradable bioactive porous PDLLA/Bioglass composite scaffold with a high-potential stem cell source (human ADSCs) could be a promising approach for bone regeneration in a clinical setting.
authors.affiliation: Research and Development Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, 145 West Changle Road, Xi'an, Shaanxi, 710032, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.forename: Kun
authors.initials: K
authors.lastname: Ji
authors.forename: Jennifer
authors.initials: J
authors.lastname: Kirkham
authors.forename: Yu
authors.initials: Y
authors.lastname: Yan
authors.forename: Aldo R
authors.initials: AR
authors.lastname: Boccaccini
authors.forename: Margaret
authors.initials: M
authors.lastname: Kellett
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
authors.forename: Xuebin B
authors.initials: XB
authors.lastname: Yang
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001842:Bone and Bones / Q000187:drug effects / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002516:Ceramics / Q000494:pharmacology*; D003094:Collagen / Q000096:biosynthesis; D006801:Humans; D007150:Immunohistochemistry; D019344:Lactic Acid / Q000494:pharmacology*; D008297:Male; D051379:Mice; D011091:Polyesters; D011108:Polymers / Q000494:pharmacology*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: C022107:Bioglass; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D003094:Collagen; D000469:Alkaline Phosphatase