title: High expression of TNFSF13 in tumor cells and fibroblasts is associated with poor prognosis in non-small cell lung cancer.
abstract: OBJECTIVE
  To examine high expression of tumor necrosis factor ligand superfamily member 13 (TNFSF13), which is correlated with several malignancies.
  
  
  METHODS
  TNFSF13 messenger RNA expression in tumor cells and fibroblasts in a cohort of patients with non-small cell lung cancer (NSCLC) was analyzed by quantitative real-time polymerase chain reaction and immunohistochemistry using a tissue microarray.
  
  
  RESULTS
  TNFSF13 expression was significantly higher in lung adenocarcinomas compared with squamous cell carcinomas (P = .022). High TNFSF13 expression in NSCLC stroma was related with low differentiation (P = .045) and sex (male > female, P = .005). Cox proportional hazards regression univariate and multivariable analysis revealed TNFSF13 expression in NSCLC tumor cells (P = .007) or fibroblasts (P = .027) as an independent prognostic factor in the 5-year overall survival rate.
  
  
  CONCLUSIONS
  Our findings indicate TNFSF13 is a prognostic factor in NSCLC and suggest TNFSF13 may be a novel therapeutic target for NSCLC.
authors.affiliation: Hospital of Integrated Traditional Chinese and Western Medicine of Zhejiang Province, Tuberculosis Diagnosis and Treatment Center of Zhejiang Province, 310003, China; zhumindoctor@163.com.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Qian
authors.forename: Cai
authors.initials: C
authors.lastname: Qingshan
authors.forename: Jin
authors.initials: J
authors.lastname: Chun
authors.forename: Zhu
authors.initials: Z
authors.lastname: Huijun
authors.forename: Li
authors.initials: L
authors.lastname: Feng
authors.forename: Wei
authors.initials: W
authors.lastname: Qiang
authors.forename: Xia
authors.initials: X
authors.lastname: Qiang
authors.forename: Zhu
authors.initials: Z
authors.lastname: Min
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D016016:Proportional Hazards Models; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D046888:Tissue Array Analysis; D053300:Tumor Necrosis Factor Ligand Superfamily Member 13 / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; C114631:TNFSF13 protein, human; D053300:Tumor Necrosis Factor Ligand Superfamily Member 13
keywords: Immunohistochemistry; Non–small cell lung cancer; Overall survival; Tissue microarray; Tumor necrosis factor ligand superfamily member 13 (TNFSF13)