title: Collagen XXII binds to collagen-binding integrins via the novel motifs GLQGER and GFKGER.
abstract: Collagen XXII, a FACIT (fibril-associated collagen with interrupted triple helices), is expressed at the myotendinous junction and the articular surface of joint cartilage. Cellular receptors like collagen-binding integrins are known to bind collagens with distinct binding motifs following the sequence GXOGER. In the present study, we demonstrate the sequences GLQGER and GFKGER as novel binding motifs between collagen XXII and collagen-binding integrins, especially α2β1 integrin. Solid-phase assays and surface plasmon resonance spectroscopy revealed a direct interaction between α2β1 integrin and the motif GFKGER. In addition, immunohistochemical analysis demonstrated partial co-localization of collagen XXII, α2β1 integrin and α11β1 integrin at the myotendinous junction. Furthermore, computational modelling of the motifs GLQGER and GFKGER showed perfect fitting of the sequences into the binding pocket of collagen-binding integrins. Taken together, we demonstrated that collagen XXII interacts with collagen-binding integrins via the new motifs GLQGER and GFKGER.
authors.forename: Daniela
authors.initials: D
authors.lastname: Zwolanek
authors.affiliation: *Center for Biochemistry, Medical Faculty, University of Cologne, 50931 Cologne, Germany.
authors.forename: Guido
authors.initials: G
authors.lastname: Veit
authors.affiliation: ‡Center for Molecular Medicine, Department of Vascular Biology, Excellence Cluster Cardio-Pulmunary System, Frankfurt University Hospital, 60590 Frankfurt am Main, Germany.
authors.forename: Johannes A
authors.initials: JA
authors.lastname: Eble
authors.affiliation: §Department of Biomedicine, University of Bergen, NO-5009 Bergen, Norway.
authors.forename: Donald
authors.initials: D
authors.lastname: Gullberg
authors.affiliation: ∥Institut de Génomique Fonctionnelle de Lyon, ENS de Lyon, UMR CNRS 5242, Université Lyon 1, 46 Allée d'Italie, 69364 Lyon, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Ruggiero
authors.affiliation: ¶Department of Biochemistry and Food Chemistry, University of Turku, Turku FI-20014, Finland.
authors.forename: Jyrki
authors.initials: J
authors.lastname: Heino
authors.affiliation: **Department of Chemistry, University of Manitoba, Winnipeg, Manitoba R2T 2N2, Canada.
authors.forename: Markus
authors.initials: M
authors.lastname: Meier
authors.affiliation: ††Department of Chemistry, Microbiology and Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Manitoba R2T 2N2, Canada.
authors.forename: Jörg
authors.initials: J
authors.lastname: Stetefeld
authors.forename: Manuel
authors.initials: M
authors.lastname: Koch
mesh_terms: D020816:Amino Acid Motifs / Q000502:physiology; D000818:Animals; D016466:CHO Cells; D045744:Cell Line, Tumor; D003412:Cricetulus; D024021:Fibril-Associated Collagens / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D016023:Integrins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D011485:Protein Binding / Q000502:physiology; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
chemical_list: D024021:Fibril-Associated Collagens; D016023:Integrins