title: Type V collagen fibrils in mouse metanephroi.
abstract: Type V collagen (Col V) molecule, a minor component of kidney connective tissues, was found in adult cornea, and has been considered as a regulatory fibril-forming collagen that emerges into type I collagen to trigger the initiation of Col I fiber assembly. Col V was also found in injured, wound healing tissues or placenta, and was considered as a dysfunctional extracellular matrix (ECM). Reconstituted Col V fibril was characterized as an ECM to detach cells in vitro, and our previous study showed that the reconstituted Col V fibril facilitated the migration of glomerular endothelial cells and induced ECM remodeling, whereas Col V molecules stabilized cells. These facts suggest that not only the structure but also the function of Col V fibril are different from Col V molecule. Recently, Col V molecule has been reported existing in various developing tissues such as bone and lung, but Col V fibril has not been reported yet. In this study, we firstly explored the existence of Col V fibril in metanephroi, and found it distributed in the immature kidney tissues whereas disappeared when the tissues reached mature. It is likely that Col V fibril may form a prototype of pericellular microenvironment and the transient existence of Col V fibril may play a role as the pioneering ECM during metanephric tissue morphogenesis.
authors.forename: Han-Hsiu
authors.initials: HH
authors.lastname: Hsu
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Murasawa
authors.forename: Pan
authors.initials: P
authors.lastname: Qi
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Nishimura
authors.forename: Pi-Chao
authors.initials: PC
authors.lastname: Wang
mesh_terms: D000818:Animals; D060833:Cellular Microenvironment; D024042:Collagen Type I / Q000378:metabolism; D024062:Collagen Type V / Q000378:metabolism*; D005109:Extracellular Matrix; D005260:Female; D007668:Kidney / Q000196:embryology* / Q000378:metabolism*; D007678:Kidney Glomerulus / Q000196:embryology / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR
chemical_list: D024042:Collagen Type I; D024062:Collagen Type V