title: Chemoenzymatic synthesis of ADP-d-glycero-β-d-manno-heptose and study of the substrate specificity of HldE.
abstract: An efficient one-pot three enzymes strategy for chemoenzymatic synthesis of ADP-d-glycero-β-d-manno-heptose (ADP-d, d-heptose) was reported using chemically synthesized d, d-heptose-7-phosphate and the ADP-d, d-heptose biosynthetic enzymes HldE and GmhB. Moreover, the result of investigating substrate specificity of the kinase action of HldE revealed that HldE had highly restricted substrate specificity towards structurally modified heptose-7-phosphate analogs.
authors.affiliation: Department of Chemistry, Center for Therapeutics and Diagnostics, Georgia State University, Atlanta, GA 30303, USA.
authors.forename: Tiehai
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Chemistry, Center for Therapeutics and Diagnostics, Georgia State University, Atlanta, GA 30303, USA.
authors.forename: Liuqing
authors.initials: L
authors.lastname: Wen
authors.affiliation: Department of Chemistry, Center for Therapeutics and Diagnostics, Georgia State University, Atlanta, GA 30303, USA.
authors.forename: Adriel
authors.initials: A
authors.lastname: Williams
authors.affiliation: Department of Chemistry, Center for Therapeutics and Diagnostics, Georgia State University, Atlanta, GA 30303, USA.
authors.forename: Baolin
authors.initials: B
authors.lastname: Wu
authors.affiliation: Department of Chemistry, Center for Therapeutics and Diagnostics, Georgia State University, Atlanta, GA 30303, USA.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Chemistry, Center for Therapeutics and Diagnostics, Georgia State University, Atlanta, GA 30303, USA.
authors.forename: Jingyao
authors.initials: J
authors.lastname: Qu
authors.affiliation: Department of Chemistry, Center for Therapeutics and Diagnostics, Georgia State University, Atlanta, GA 30303, USA.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Meisner
authors.affiliation: Department of Chemistry, Center for Therapeutics and Diagnostics, Georgia State University, Atlanta, GA 30303, USA.
authors.forename: Zhongying
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: National Glycoengineering Research Center, Shandong University, Jinan, Shandong 250100, People's Republic of China.
authors.forename: Junqiang
authors.initials: J
authors.lastname: Fang
authors.affiliation: Department of Chemistry, Center for Therapeutics and Diagnostics, Georgia State University, Atlanta, GA 30303, USA; National Glycoengineering Research Center, Shandong University, Jinan, Shandong 250100, People's Republic of China. Electronic address: pwang11@gsu.edu.
authors.forename: Peng George
authors.initials: PG
authors.lastname: Wang
mesh_terms: D000247:Adenosine Diphosphate Sugars / Q000138:chemical synthesis* / Q000378:metabolism; D060326:Chemistry Techniques, Synthetic; D009097:Multienzyme Complexes / Q000378:metabolism*; D009713:Nucleotidyltransferases / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D013379:Substrate Specificity; D013403:Sugar Phosphates / Q000737:chemistry
chemical_list: D000247:Adenosine Diphosphate Sugars; D009097:Multienzyme Complexes; D013403:Sugar Phosphates; D017853:Phosphotransferases (Alcohol Group Acceptor); D009713:Nucleotidyltransferases; C445302:D,D heptose 7-phosphate kinase-D,D-heptose 1-phosphate adenylyltransferase, E coli
keywords: Analogs; Chemoenzymatic; Specificity; Synthesis