title: A novel colorimetric method for the detection of Escherichia coli using cytochrome c peroxidase-encoding bacteriophage.
abstract: A new rapid and simple method was developed for the detection of Escherichia coli by constructing a recombinant T4 phage carrying the cytochrome c peroxidase gene derived from Saccharomyces cerevisiae (T4ccp) using which, the colorimetric detection of E. coli K12 was examined. The oxidation activity toward the chromogenic substrate cytochrome c was demonstrated by the cytochrome c peroxidase (CCP) produced from the T4ccp genome. The color change caused by the oxidation of the substrate could be visually perceived. The possibility of interference in the detection by the coexistence of other bacteria was assessed using Pseudomonas aeruginosa as a nontarget bacterium, and it was confirmed that the coexistence of P. aeruginosa caused no interference in the detection of E. coli K12.
authors.affiliation: Department of International Development Engineering, Tokyo Institute of Technology, Meguro-ku, Tokyo, Japan.
authors.forename: Hoang A
authors.initials: HA
authors.lastname: Hoang
authors.forename: Michiharu
authors.initials: M
authors.lastname: Abe
authors.forename: Kiyohiko
authors.initials: K
authors.lastname: Nakasaki
mesh_terms: D017122:Bacteriophage T4 / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D003124:Colorimetry / Q000379:methods*; D003578:Cytochrome-c Peroxidase / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry / Q000302:isolation & purification* / Q000821:virology*; D005656:Fungal Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
chemical_list: D005656:Fungal Proteins; D003578:Cytochrome-c Peroxidase
keywords: Escherichia coli; T4 bacteriophage; colorimetric detection; cytochrome c peroxidase