title: A simple procedure to obtain plasmids suitable for transfecting mammalian cell lines.
abstract: A simple procedure to obtain plasmid preparations, suitable for transfecting mammalian cell lines using a calcium phosphate co-precipitation technique, is described. The protocol is based on the purification of plasmid DNA by double gel-filtration chromatography on Sephacryl S-1000 and additional slight modifications to the original transfection procedure. The purity of plasmid preparation was verified by analytical methods. The resulting preparation efficiently transfected NIH-3T3 cells.
authors.affiliation: , .
authors.forename: L
authors.initials: L
authors.lastname: López-Cánovas
authors.forename: M
authors.initials: M
authors.lastname: Rodríguez
authors.forename: A M
authors.initials: AM
authors.lastname: Riverón Rojas