title: Hepatoprotective effects of Yulangsan polysaccharide against isoniazid and rifampicin-induced liver injury in mice.
abstract: BACKGROUND
  Yulangsan polysaccharide (YLSPS) is often used in popular folk medicine in the Guangxi Zhuang Autonomous Region of China as a chief ingredient of Millettia pulchra, which is used as an hepatic protection, anti-aging and memory improving agent. In this study, the hepatoprotective effects of YLSPS against isoniazid (INH) or rifampicin and isoniazid (RFP+INH)-induced liver injury were investigated in mice.
  
  
  METHODS
  The liver injury was induced by intragastric administration of INH or RFP+INH daily for 10 days. During the experiment, the model group received INH or RFP+INH only, and the normal control group received an equal volume of saline. Treatment groups received not only INH or RFP+INH but also the corresponding drugs, DDB (200mg/kg/day) or YLSPS (100, 200, and 400mg/kg/day) 2h after the administration of INH or RFP+INH.
  
  
  RESULTS
  Analysis experiments showed that YLSPS significantly alleviated liver injury as indicated by the decreased levels of ALT and AST and the increased levels of SOD, GSH and GSH-Px. Moreover, YLSPS could effectively reduce the pathological tissue damage. The research on the mechanisms underlying the hepatoprotective effect showed that YLSPS was able to reduce lipid peroxidation and activate the anti-oxidative defense system.
  
  
  CONCLUSIONS
  Our results show that YLSPS is effective in attenuating hepatic injury in the INH or RFP+INH-induced mouse model, and could be developed as a new drug for treatment of liver injury.
authors.affiliation: Department of Pharmacology, Guangxi Medical University, Nanning 530021, China.
authors.forename: Yizhu
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Department of Pharmacology, Guangxi Medical University, Nanning 530021, China.
authors.forename: Jianchun
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Pharmacology, Guangxi Medical University, Nanning 530021, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Lin
authors.affiliation: Department of Pharmacology, Guangxi Medical University, Nanning 530021, China.
authors.forename: Shijun
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology, Guangxi Medical University, Nanning 530021, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Jiao
authors.affiliation: Department of Pharmacology, Guangxi Medical University, Nanning 530021, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Liang
authors.affiliation: Department of Pharmacology, Guangxi Medical University, Nanning 530021, China.
authors.forename: Zhaoni
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Pharmacology, Guangxi Medical University, Nanning 530021, China. Electronic address: huangrenbin518@163.com.
authors.forename: Renbin
authors.initials: R
authors.lastname: Huang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D000995:Antitubercular Agents / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D005260:Female; D007538:Isoniazid / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D033641:Millettia / Q000737:chemistry*; D011134:Polysaccharides / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D012293:Rifampin / Q000633:toxicity
chemical_list: D000975:Antioxidants; D000995:Antitubercular Agents; D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; D007538:Isoniazid; D012293:Rifampin
keywords: Hepatoprotective effects; Isoniazid; Rifampin; Yulangsan polysaccharide (YLSPS)