title: Comparison of ChromID agar and Clostridium difficile selective agar for effective isolation of C. difficile from stool specimens.
abstract: BACKGROUND
  ChromID Clostridium difficile agar (IDCd; bioMérieux SA, France) is a recently developed chromogenic medium for rapid and specific isolation of C. difficile. We compared the performance of IDCd with that of Clostridium difficile Selective Agar (CDSA).
  
  
  METHODS
  A total of 530 fresh stool specimens were collected from patients with clinical signs compatible with C. difficile infection, and cultures for C. difficile were performed on IDCd and CDSA. C. difficile colonies were identified by spore staining, odor, use of an ANI identification test kit (bioMérieux SA), and multiplex PCR for tcdA, tcdB, and tpi.
  
  
  RESULTS
  The concordance rate between IDCd and CDSA was 90.6% (480/530). The positivity rates on IDCd on days 1 and 2 (55.6% and 85.0%, respectively) were significantly higher than those on CDSA (19.4% and 75.6%, respectively) (P<0.001 for day 1 and P=0.02 for day 2), but the detection rates on IDCd and CDSA on day 3 were not different (89.4% vs. 82.8%, P=0.0914). On day 3, the recovery rates for non-C. difficile isolates on IDCd and CDSA were 30.2% (160/530) and 22.1% (117/530), respectively (P=0.0075). Clostridium spp. other than C. difficile were the most prevalent non-C. difficile isolates on both media.
  
  
  CONCLUSIONS
  The culture positivity rates on IDCd and CDSA were not different on day 3 but IDCd may allow for rapid and sensitive detection of C. difficile within 2 days of cultivation.
authors.affiliation: Department of Laboratory Medicine, Inje University, Sanggye Paik Hospital, Seoul, Korea.
authors.forename: Bo-Moon
authors.initials: BM
authors.lastname: Shin
authors.affiliation: Department of Laboratory Medicine, Inje University, Sanggye Paik Hospital, Seoul, Korea.
authors.forename: Eun Joo
authors.initials: EJ
authors.lastname: Lee
mesh_terms: D000362:Agar / Q000737:chemistry*; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D016360:Clostridioides difficile / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis; D004761:Enterocolitis, Pseudomembranous / Q000175:diagnosis / Q000382:microbiology; D004768:Enterotoxins / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic; D014305:Triose-Phosphate Isomerase / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004768:Enterotoxins; D011933:Reagent Kits, Diagnostic; C041348:tcdA protein, Clostridium difficile; C031815:toxB protein, Clostridium difficile; D000362:Agar; D014305:Triose-Phosphate Isomerase
keywords: Chromogenic agar; Clostridium difficile; Clostridium difficile selective agar; Culture