title: Multistructural biomimetic substrates for controlled cellular differentiation.
abstract: Multidimensional scaffolds are considered to be ideal candidates for regenerative medicine and tissue engineering based on their potential to provide an excellent microenvironment and direct the fate of the cultured cells. More recently, the use of stem cells in medicine has opened a new technological opportunity for controlled tissue formation. However, the mechanism through which the substrate directs the differentiation of stem cells is still rather unclear. Data concerning its specific surface chemistry, topology, and its signaling ability need to be further understood and analyzed. In our study, atomic force microscopy was used to study the stiffness, roughness, and topology of the collagen (Coll) and metallized collagen (MC) substrates, proposed as an excellent substrate for regenerative medicine. The importance of signaling molecules was studied by constructing a new hybrid signaling substrate that contains both collagen and laminin extracellular matrix (ECM) proteins. The cellular response-such as attachment capability, proliferation and cardiac and neuronal phenotype expression on the metallized and non-metallized hybrid substrates (collagen + laminin)-was studied using MTT viability assay and immunohistochemistry studies. Our findings indicate that such hybrid materials could play an important role in the regeneration of complex tissues.
authors.affiliation: Center for Integrative Nanotechnology Sciences, University of Arkansas at Little Rock, 2801 South University Avenue, Little Rock, AR 72204, USA. The Oncology Institute, Prof. Dr. I. Chiricuta, Republicii, No. 34-36, RO-400015, Cluj-Napoca, Romania.
authors.forename: Anamaria I
authors.initials: AI
authors.lastname: Orza
authors.forename: Carmen
authors.initials: C
authors.lastname: Mihu
authors.forename: Olga
authors.initials: O
authors.lastname: Soritau
authors.forename: Mircea
authors.initials: M
authors.lastname: Diudea
authors.forename: Adrian
authors.initials: A
authors.lastname: Florea
authors.forename: Horea
authors.initials: H
authors.lastname: Matei
authors.forename: Stefana
authors.initials: S
authors.lastname: Balici
authors.forename: Thilak
authors.initials: T
authors.lastname: Mudalige
authors.forename: Ganesh K
authors.initials: GK
authors.lastname: Kanarpardy
authors.forename: Alexandru S
authors.initials: AS
authors.lastname: Biris
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D032701:Biomimetics; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects*; D002470:Cell Survival; D003094:Collagen / Q000737:chemistry; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007797:Laminin / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry; D018625:Microscopy, Atomic Force; D032383:Myocytes, Cardiac / Q000166:cytology; D009474:Neurons / Q000187:drug effects; D010641:Phenotype; D056951:Photoelectron Spectroscopy; D012038:Regeneration; D044968:Regenerative Medicine / Q000379:methods*; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D007797:Laminin; D003094:Collagen