title: Properties and intracellular distribution of two phosphoglucomutases from spinach leaves.
abstract: Two isoenzymes of phosphoglucomutase from spinach (Spinacia oleracea L.) leaves can be separated by ammonium-sulfate gradient solubilization or DEAE-cellulose ion exchange chromatography. They were designated as phosphoglucomutase 1 and 2, according to decreasing electrophoretic mobility towards the anode at pH 8.9. Phosphoglucomutase 1 is localized in the stroma of the chloroplasts, phosphoglucomutase 2 is a cytosolic enzyme as judged from aqueous cell fractionation studies. Both isoenzymes have very similar properties such as dependence on MgCl2, pH activity profile, and Km for glucose-1-phosphate and glucose-1,6-bisphosphate. From sedimentation-velocity analysis a molecular weight of 60,000 was estimated for either isoenzyme.
authors.affiliation: Fachbereich Biologie, Universität Kaiserslautern, Pfaffenbergstraße, D-6750, Kaiserslautern, Federal Republic of Germany.
authors.forename: H
authors.initials: H
authors.lastname: Mühlbach
authors.forename: C
authors.initials: C
authors.lastname: Schnarrenberger