title: CDK11(p58) kinase activity is required to protect sister chromatid cohesion at centromeres in mitosis.
abstract: The cyclin-dependent kinase CDK11(p58) is specifically expressed at G2/M phase. CDK11(p58) depletion leads to different cell cycle defects such as mitotic arrest, failure in centriole duplication and centrosome maturation, and premature sister chromatid separation. We report that upon CDK11 depletion, loss of sister chromatid cohesion occurs during mitosis but not during G2 phase. CDK11(p58) depletion prevents Bub1 and Shugoshin 1 recruitment but has no effect on the dimethylation of histone H3 lysine 4 at centromeres. We also report that a construct expressing a kinase dead version of CDK11(p58) fails to prevent CDK11 depletion-induced sister chromatid separation, showing that CDK11(p58) kinase activity is required for protection of sister chromatid cohesion at centromeres during mitosis. Thus, CDK11(p58) kinase activity appears to be involved in early events in the establishment of the centromere protection machinery.
authors.affiliation: Institut de Génétique et Développement de Rennes, Equipe Epigénétique et Cancer UMR 6290 CNRS, Université Rennes 1, 35043, Rennes Cedex, France.
authors.forename: Tarik
authors.initials: T
authors.lastname: Rakkaa
authors.forename: Christophe
authors.initials: C
authors.lastname: Escudé
authors.forename: Régis
authors.initials: R
authors.lastname: Giet
authors.forename: Laura
authors.initials: L
authors.lastname: Magnaghi-Jaulin
authors.forename: Christian
authors.initials: C
authors.lastname: Jaulin
mesh_terms: D018797:Cell Cycle Proteins / Q000378:metabolism; D002503:Centromere / Q000378:metabolism*; D002842:Chromatids / Q000378:metabolism*; D056743:Cyclin D3 / Q000378:metabolism*; D016195:G2 Phase; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D012854:Sister Chromatid Exchange
chemical_list: C534696:CCND3 protein, human; D018797:Cell Cycle Proteins; D056743:Cyclin D3; C496644:SGO1 protein, human; C584277:BUB1 protein, human; D017346:Protein Serine-Threonine Kinases