title: Nociception and inflammatory hyperalgesia evaluated in rodents using infrared laser stimulation after Trpv1 gene knockout or resiniferatoxin lesion.
abstract: TRPV1 is expressed in a subpopulation of myelinated Aδ and unmyelinated C-fibers. TRPV1+ fibers are essential for the transmission of nociceptive thermal stimuli and for the establishment and maintenance of inflammatory hyperalgesia. We have previously shown that high-power, short-duration pulses from an infrared diode laser are capable of predominantly activating cutaneous TRPV1+ Aδ-fibers. Here we show that stimulating either subtype of TRPV1+ fiber in the paw during carrageenan-induced inflammation or following hind-paw incision elicits pronounced hyperalgesic responses, including prolonged paw guarding. The ultrapotent TRPV1 agonist resiniferatoxin (RTX) dose-dependently deactivates TRPV1+ fibers and blocks thermal nociceptive responses in baseline or inflamed conditions. Injecting sufficient doses of RTX peripherally renders animals unresponsive to laser stimulation even at the point of acute thermal skin damage. In contrast, Trpv1-/- mice, which are generally unresponsive to noxious thermal stimuli at lower power settings, exhibit withdrawal responses and inflammation-induced sensitization using high-power, short duration Aδ stimuli. In rats, systemic morphine suppresses paw withdrawal, inflammatory guarding, and hyperalgesia in a dose-dependent fashion using the same Aδ stimuli. The qualitative intensity of Aδ responses, the leftward shift of the stimulus-response curve, the increased guarding behaviors during carrageenan inflammation or after incision, and the reduction of Aδ responses with morphine suggest multiple roles for TRPV1+ Aδ fibers in nociceptive processes and their modulation of pathological pain conditions.
authors.affiliation: Department of Perioperative Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, USA Department of Anesthesia, Stanford University, Palo Alto, CA, USA Lasmed LLC, Mountain View, CA, USA Neurobiology and Pain Therapeutics Section, Laboratory of Sensory Biology, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA.
authors.forename: Kendall
authors.initials: K
authors.lastname: Mitchell
authors.forename: Evan E
authors.initials: EE
authors.lastname: Lebovitz
authors.forename: Jason M
authors.initials: JM
authors.lastname: Keller
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Mannes
authors.forename: Michael I
authors.initials: MI
authors.lastname: Nemenov
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Iadarola
mesh_terms: D000701:Analgesics, Opioid / Q000627:therapeutic use; D000818:Animals; D002351:Carrageenan / Q000633:toxicity; D004195:Disease Models, Animal; D004224:Diterpenes / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D006930:Hyperalgesia / Q000188:drug therapy / Q000209:etiology*; D007249:Inflammation / Q000150:complications* / Q000209:etiology; D007834:Lasers / Q000009:adverse effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009020:Morphine / Q000627:therapeutic use; D009498:Neurotoxins / Q000633:toxicity*; D059225:Nociception / Q000502:physiology*; D017288:Pain Threshold / Q000187:drug effects / Q000502:physiology; D010812:Physical Stimulation / Q000009:adverse effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D050916:TRPV Cation Channels / Q000235:genetics / Q000378:metabolism*
chemical_list: D000701:Analgesics, Opioid; D004224:Diterpenes; D009498:Neurotoxins; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse; D009020:Morphine; D002351:Carrageenan; C024353:resiniferatoxin
keywords: Analgesia; Capsaicin; Chemoreceptors; Chronic Pain; Nociception