title: Regulation of skeletal muscle protein degradation and synthesis by oral administration of lysine in rats.
abstract: Several catabolic diseases and unloading induce muscle mass wasting, which causes severe pathological progression in various diseases and aging. Leucine is known to attenuate muscle loss via stimulation of protein synthesis and suppression of protein degradation in skeletal muscle. The aim of this study was to investigate the effects of lysine intake on protein degradation and synthesis in skeletal muscle. Fasted rats were administered 22.8-570 mg Lys/100 g body weight and the rates of myofibrillar protein degradation were assessed for 0-6 h after Lys administration. The rates of myofibrillar protein degradation evaluated by MeHis release from the isolated muscles were markedly suppressed after administration of 114 mg Lys/100 g body weight and of 570 mg Lys/100 g body weight. LC3-II, a marker of the autophagic-lysosomal pathway, tended to decrease (p=0.05, 0.08) after Lys intake (114 mg/100 g body weight). However, expression of ubiquitin ligase E3 atrogin-1 mRNA and levels of ubiquitinated proteins were not suppressed by Lys intake. Phosphorylation levels of mTOR, S6K1 and 4E-BP1 in the gastrocnemius muscle were not altered after Lys intake. These results suggest that Lys is able to suppress myofibrillar protein degradation at least partially through the autophagic-lysosomal pathway, not the ubiquitin-proteasomal pathway, whereas Lys might be unable to stimulate protein synthesis within this time frame.
authors.affiliation: Biological Chemistry and Food Science, Graduate School of Agriculture, Iwate University.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Sato
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Ito
authors.forename: Takashi
authors.initials: T
authors.lastname: Nagasawa
mesh_terms: D000818:Animals; D001343:Autophagy; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D019587:Dietary Supplements; D018507:Gene Expression Regulation, Developmental; D007700:Kinetics; D008239:Lysine / Q000008:administration & dosage* / Q000097:blood / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism; D008297:Male; D008869:Microtubule-Associated Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D024510:Muscle Development; D009124:Muscle Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000254:growth & development / Q000378:metabolism*; D009210:Myofibrils / Q000378:metabolism; D014176:Protein Biosynthesis; D055550:Protein Stability; D059748:Proteolysis; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D015415:Biomarkers; D008239:Lysine; D008869:Microtubule-Associated Proteins; D009124:Muscle Proteins; C526109:Anxa3 protein, rat