title: Antioxidant role of selenium in rats with experimental acute otitis media.
abstract: The aim of this prospective experimental animal study was to determine whether selenium had a protective effect on oxidative stress in rats with acute otitis media, by measuring the alterations of antioxidant parameters and lipid peroxidation on days 4 and 10 after inoculation into the middle ear. Streptococcus pneumoniae was inoculated into the middle ear cavities of 32 rats in animal laboratory of a tertiary medical center. Group 1 served as the control group and the animals were administered 1.5 ml/day saline. Group 2 received 0.2 mg/kg/day oral selenium for 10 days. The blood samples of each group were obtained on post-inoculation days 4 and 10. The levels of thiobarbituric acid reactive substances, albumin, total sulphydryl, superoxide dismutase and glutathione peroxidase were investigated. Day 10 level of thiobarbituric acid reactive substance in group 2 was lower than the day 4 level of the same substance in the control group. Although glutathione peroxidase and superoxide dismutase levels significantly decreased starting from 4th day until 10th day in group 1, their levels increased in group 2. Day 10 levels of albumin and total sulphydryl in group 1 were significantly higher than day 4 levels in group 2. We found that selenium supplementation for 10 days decreased thiobarbituric acid reactive substances and increased glutathione peroxidase and superoxide dismutase levels when compared to the control group. We believe that selenium supplementation may be beneficial to prevent the clinical sequelae and recurrence of otitis media.
authors.affiliation: Department of Otorhinolaryngology, Ankara Training and Research Hospital, Konutkent Mah. Oyak 1 Blok 20/9 Çayyolu, Ankara, Turkey.
authors.forename: Filiz
authors.initials: F
authors.lastname: Aydoğan
authors.affiliation: Department of Otorhinolaryngology, Ankara Training and Research Hospital, Ankara, Turkey.
authors.forename: Eren
authors.initials: E
authors.lastname: Taştan
authors.affiliation: Department of Otorhinolaryngology, Ankara Training and Research Hospital, Ankara, Turkey.
authors.forename: Emine
authors.initials: E
authors.lastname: Aydın
authors.affiliation: Department of Biochemistry, Ankara Training and Research Hospital, Ankara, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Senes
authors.affiliation: Department of Pathology, Ankara Training and Research Hospital, Ankara, Turkey.
authors.forename: Sükran
authors.initials: S
authors.lastname: Akgedik
authors.affiliation: Department of Microbiology, Ankara Training and Research Hospital, Ankara, Turkey.
authors.forename: Rukiye
authors.initials: R
authors.lastname: Berkem
authors.affiliation: Department of Animal Science Feeds and Animal Nutrition, Faculty of Agriculture, Yuzuncu Yil University, Van, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Tekeli
authors.affiliation: Department of Biochemistry, Ankara Training and Research Hospital, Ankara, Turkey.
authors.forename: Aylin
authors.initials: A
authors.lastname: Haklıgör
authors.affiliation: Department of Pathology, Ankara Training and Research Hospital, Ankara, Turkey.
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Ustün
keywords: Antioxidant; Glutathione peroxidase; Otitis media; Rats; Selenium