title: The copper (II) ion as a carrier for the antibiotic capreomycin against Mycobacterium tuberculosis.
abstract: In recent years, the bacterium responsible for tuberculosis has been increasing its resistance to antibiotics resulting in new multidrug resistant Mycobacterium tuberculosis (MR-TB) and extensively drug-resistant tuberculosis (XDR-TB). In this study we use several analytical techniques including NMR, FT-ICR, TOF-MS, LC-MS and UV/Vis to study the copper-capreomycin complex. The copper (II) cation is used as a carrier for the antibiotic capreomycin. Once this structure was studied using NMR, FT-ICR, and MALDI-TOF-MS, the NIH-NIAID tuberculosis cell line for several Tb strains (including antibiotic resistant strains) were tested against up to seven variations of the copper-capreomycin complex. Different variations of copper improved the efficacy of capreomycin against Tb up to 250 fold against drug resistant strains of Tb.
authors.affiliation: Chemistry, Valdosta State University, Valdosta, GA 31698, United States. Electronic address: tmanning@valdosta.edu.
authors.forename: Thomas
authors.initials: T
authors.lastname: Manning
authors.affiliation: Chemistry, Valdosta State University, Valdosta, GA 31698, United States.
authors.forename: Rachel
authors.initials: R
authors.lastname: Mikula
authors.affiliation: Chemistry, Valdosta State University, Valdosta, GA 31698, United States.
authors.forename: Hilary
authors.initials: H
authors.lastname: Lee
authors.affiliation: Chemistry, Valdosta State University, Valdosta, GA 31698, United States.
authors.forename: Aaron
authors.initials: A
authors.lastname: Calvin
authors.affiliation: Chemistry, Valdosta State University, Valdosta, GA 31698, United States.
authors.forename: Jarrett
authors.initials: J
authors.lastname: Darrah
authors.affiliation: NMR Facility, Chemistry, UGA, Athens, GA, United States.
authors.forename: Greg
authors.initials: G
authors.lastname: Wylie
authors.affiliation: PAMS, Chemistry, UGA, Athens, GA, United States.
authors.forename: Dennis
authors.initials: D
authors.lastname: Phillips
authors.affiliation: National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL 32310, United States.
authors.forename: Benjamin J
authors.initials: BJ
authors.lastname: Bythell
mesh_terms: D000904:Antibiotics, Antitubercular / Q000737:chemistry / Q000494:pharmacology; D002207:Capreomycin / Q000737:chemistry* / Q000494:pharmacology*; D003300:Copper / Q000737:chemistry; D024881:Drug Resistance, Bacterial / Q000187:drug effects*; D007477:Ions; D015394:Molecular Structure; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000904:Antibiotics, Antitubercular; D007477:Ions; D002207:Capreomycin; D003300:Copper
keywords: Antibiotic; Capreomycin; Drug delivery; FT-ICR; Mass spec; Mycobacterium tuberculosis; NMR; Tb