title: Partial characterization, antioxidant and antitumor activities of polysaccharides from Philomycusbilineatus.
abstract: Four polysaccharides (PBP60-A, PBP60-B, PBP60-C and PBP60-D) were purified from slug (Philomycusbilineatus) by ion-exchange chromatography. The antioxidant activities were studied by ABTS, DPPH, hydroxyl radical, superoxide radical and reducing power assay. In vitro antitumor activities were evaluated by MTT assay. Results demonstrated that PBP60-A was mainly composed of Man, Rha, Glc, Gal, Xyl and Fuc in a mole ratio of 6.13:3.08:8.97:5.22:2.46:1.13. PBP60-B was composed of Man, GlcN, Rha, GalN, GlcU, Glc, Gal, Xyl and Fuc in a mole ratio of 0.90:0.31:1.15:0.37:0.24:1.02:3.84:0.93:1.99. PBP60-C and PBP60-D were composed of Man, GlcN, Rha, GalN, GlcU, Glc, Gal, Xyl, Fuc and an unknown monosaccharide. Antioxidant tests indicated that four polysaccharides exhibited significant antioxidant activities in a dose-dependent manner. PBP60-D presented relative stronger antioxidant activity. PBP60-C showed higher antitumor activity against A549 and MCF-7 cells in vitro. At concentration of500 μg/mL, the antitumor activities of PBP60-C on theA549 and MCF-7 cells were 65.30% and 42.45%, respectively. These results indicated that polysaccharides from Philomycusbilineatus could be explored as potential natural antioxidants and cancer prevention agents.
authors.affiliation: College of Biological and Environmental Engineering,Zhejiang University of Technology, Hangzhou 310032, PR China.
authors.forename: Rongjun
authors.initials: R
authors.lastname: He
authors.affiliation: College of Biological and Environmental Engineering,Zhejiang University of Technology, Hangzhou 310032, PR China.
authors.forename: Jiaming
authors.initials: J
authors.lastname: Ye
authors.affiliation: College of Biological and Environmental Engineering,Zhejiang University of Technology, Hangzhou 310032, PR China.
authors.forename: Yuejun
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Pharmaceutical Engineering of Ministry of Education, College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, PR China. Electronic address: pharmlab@zjut.edu.cn.
authors.forename: Weike
authors.initials: W
authors.lastname: Su
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D049851:Gastropoda / Q000737:chemistry*; D006801:Humans; D009005:Monosaccharides / Q000032:analysis; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D016166:Free Radical Scavengers; D009005:Monosaccharides; D011134:Polysaccharides
keywords: Antioxidant activity; Antitumor activity; Polysaccharide; Slug (Philomycusbilineatus)