title: Fluorescence polarization of trypsin digested photosystem II membranes.
abstract: Fluorescence polarization of photosystem II particles treated with trypsin and incubated with high salt-medium (2M NaCl) was investigated. The presence of atrazine and TMPD in normal and salt-washed particles induced a decrease in the polarization ratios. Similar results were obtained at low concentrations of trypsin. On the basis of our observations we suggest that the presence of these perturbing agents causes a reorganisation of the membrane components and alters pigment-pigment and pigment-protein interactions. The results of fluorescence polarization demonstrate trypsin entry into the membrane after the digestion of the peripheral proteins.
authors.affiliation: School of Life Sciences, Jawaharlal Nehru University, 110067, New Delhi, India.
authors.forename: R
authors.initials: R
authors.lastname: Paliwal
authors.forename: G S
authors.initials: GS
authors.lastname: Singhal