title: Development of microsatellite-enriched libraries.
abstract: Among the molecular markers used for plant genetic studies, microsatellite markers are easy to implement and can provide suitable codominant markers for molecular taxonomy. Here we describe a method to obtain enriched libraries in microsatellite loci from genomic DNA, using capture method with synthetic oligonucleotide probes.
authors.affiliation: Cirad UMR AGAP, Montpellier, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Vignes
authors.forename: Ronan
authors.initials: R
authors.lastname: Rivallan
mesh_terms: D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D018900:Dinucleotide Repeats / Q000235:genetics; D056426:Genetic Loci / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics; D015698:Genomic Library; D018895:Microsatellite Repeats / Q000235:genetics*; D015345:Oligonucleotide Probes / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014170:Transformation, Genetic
chemical_list: D018744:DNA, Plant; D015345:Oligonucleotide Probes