title: White spot syndrome virus Manipulates Ubiquitin Gene Expression in Penaeus monodon.
abstract: The present study reports the effect of WSSV on the expression profiles of shrimp ubiquitin gene both at protein and transcript levels at different time points after infection. A gradual decrease in ubiquitin protein levels was observed till 12 hpi (0.25 and 0.23 μg/mg of the total protein in gills and muscle, respectively) with a sudden increase at 18 hpi (1.86 and 1.8 μg/mg of total protein in gills and muscle, respectively). The RT-PCR results showed a gradual decrease in ubiquitin mRNA levels in gill tissue till 12 hpi while a more or less constant mRNA levels were observed in muscle tissue till 12 hpi. However, a fourfold and 16 fold induction in ubiquitin mRNA levels at 18 hpi in gill and muscle, respectively suggests its possible regulation by WSSV in the manifestation of the disease.
authors.affiliation: Division of Aquatic Environment and Health Management, Central Institute of Fisheries Education, ICAR, Mumbai, India.
authors.forename: R
authors.initials: R
authors.lastname: Vidya
authors.affiliation: Division of Fish Genetics and Biotechnology, Central Institute of Fisheries Education, Indian Council of Agricultural Research, Versova, Mumbai, 400061 India.
authors.forename: P
authors.initials: P
authors.lastname: Gireesh-Babu
authors.affiliation: Division of Aquatic Environment and Health Management, Central Institute of Fisheries Education, ICAR, Mumbai, India.
authors.forename: K
authors.initials: K
authors.lastname: Pani Prasad
keywords: ELISA; Expression profiling; Penaeus monodon; Semi-quantitative RT-PCR; Ubiquitin; WSSV