title: Role of NF-κB as a Prognostic Marker in Breast Cancer : A Pilot Study in Indian Patients.
abstract: The nuclear factor κB (NF-κB) is a superfamily of transcription factors. It plays an important role in development & progression of cancer. This study was conducted in a tertiary care centre to investigate the significance of NF-κB as a prognostic marker in breast cancer and study its relation with established prognostic markers such as tumor grade, lymph node status, hormone receptor & HER-2/neu expression. We measured NF-κB expression of breast cancer tissue as a test sample & from fibroadenoma as a control. Measurement was done by Western Blot Technique using p65 protein of NF-κB super family of transcription factors. ER,PR and HER-2/neu were measured by immunohistochemistry methods. NF-κB/p65 is significantly associated with large tumor size (≥5 cm), high grade tumors, negative ER, negative PR, positive HER-2/neu and high NPI (≥5.4) scores. NF-κB/p65 expression implies aggressive biological behaviour of breast cancer & this study validates significant association of NF-κB /p65 overexpression with large tumor size, negative estrogen & progesterone receptor status and overexpression of c-erbB2 oncoprotein.
authors.affiliation: Comprehensive Breast Clinic Service & Breast Cancer Research Unit, IPGME&R & SSKM Hospital, Kolkata, West Bengal India ; 396/3,N.S.C.Bose Road, Kolkata, 700047 West Bengal India.
authors.forename: D K
authors.initials: DK
authors.lastname: Sarkar
authors.affiliation: Comprehensive Breast Clinic Service & Breast Cancer Research Unit, IPGME&R & SSKM Hospital, Kolkata, West Bengal India ; 1, Thakurbari Road, P.O.Kalighat, Kolkata, 700026 West Bengal India.
authors.forename: Debarshi
authors.initials: D
authors.lastname: Jana
authors.affiliation: Comprehensive Breast Clinic Service & Breast Cancer Research Unit, IPGME&R & SSKM Hospital, Kolkata, West Bengal India ; Junior Doctors' Hostel, Room No. 335, 242,A.J.C.Bose Road, Kolkata, 700020 West Bengal India.
authors.forename: P S
authors.initials: PS
authors.lastname: Patil
authors.affiliation: Comprehensive Breast Clinic Service & Breast Cancer Research Unit, IPGME&R & SSKM Hospital, Kolkata, West Bengal India ; Type 5, Bldg. No. 5, Flat No. 20, R.C.F. Colony, Officers' Qtrs., Chembur, Mumbai, 400074 Maharashtra India.
authors.forename: K S
authors.initials: KS
authors.lastname: Chaudhari
authors.affiliation: Comprehensive Breast Clinic Service & Breast Cancer Research Unit, IPGME&R & SSKM Hospital, Kolkata, West Bengal India ; Department of General Surgery, IPGME&R and SSKM Hospital, 244, A.J.C.Bose Road, Kolkata, West Bengal India.
authors.forename: B K
authors.initials: BK
authors.lastname: Chattopadhyay
authors.affiliation: Comprehensive Breast Clinic Service & Breast Cancer Research Unit, IPGME&R & SSKM Hospital, Kolkata, West Bengal India ; Junior Doctors' Hostel, Room No. 208, 242, A.J.C.Bose Road, Kolkata, 700020 West Bengal India.
authors.forename: B R
authors.initials: BR
authors.lastname: Chikkala
authors.affiliation: Department of Epidemiology and Biostatistics, Chittaranjan National Cancer Institute, Kolkata, West Bengal India.
authors.forename: S
authors.initials: S
authors.lastname: Mandal
authors.affiliation: Comprehensive Breast Clinic Service & Breast Cancer Research Unit, IPGME&R & SSKM Hospital, Kolkata, West Bengal India ; Department of General Surgery, IPGME&R and SSKM Hospital, 244, A.J.C.Bose Road, Kolkata, West Bengal India.
authors.forename: P
authors.initials: P
authors.lastname: Chowdhary
keywords: Breast cancer; Immunohistochemistry (IHC); NF-κB /p65 overexpression; Nottingham Prognostic Index (NPI); Nuclear factor-κB (NF-κB); Prognostic marker