title: Simultaneous preparation of naturally abundant and rare catechins by tannase-mediated biotransformation combining high speed counter current chromatography.
abstract: Simultaneous preparation of naturally rare catechins, EGC and EC, has been realized by tannase-mediated biotransformation combining high speed counter current chromatography. In addition, simultaneous preparation of the four catechins, EGCG, ECG, EGC, and EC in green tea extract has also been achieved by HSCCC under the normal phase and the reversed phase modes. The identity of the catechins was determined by HPLC-DAD-ESI-MS and quantification of the catechins was performed by HPLC-DAD. In a typical HSCCC separation, 27.2 mg 98.8% EGCG, 14.1 mg 94.7% EGC, and 9.3 mg 97.5% EC were obtained. This new method is efficient, time-saving and valuable for biological studies.
authors.affiliation: Department of Food Science and Nutrition, Fuli Institute of Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
authors.forename: Guobin
authors.initials: G
authors.lastname: Xia
authors.affiliation: Department of Food Science and Nutrition, Fuli Institute of Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Hong
authors.affiliation: Department of Food Science and Nutrition, Fuli Institute of Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China. Electronic address: songbailiu@zju.edu.cn.
authors.forename: Songbai
authors.initials: S
authors.lastname: Liu
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000737:chemistry*; D001711:Biotransformation; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry*; D002392:Catechin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013662:Tea / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D013662:Tea; D002392:Catechin; D002265:Carboxylic Ester Hydrolases; C022711:tannase
keywords: Catechin; HPLC; High speed counter current chromatography; MS; Preparation; Tannase