title: [Determination of chrysosplenetin, metabolic inhibitor of artemisinin, in rat plasma by UPLC-ms/MS and study on its pharmacokinetics].
abstract: OBJECTIVE
  The study aimed to develop the assay of chrysosplenetin (CHR), a metabolic inhibitor of artemisinin by UPLC-MS/MS in rat plasma and investigate the pharmacokinetics parameters of CHR.
  
  
  METHODS
  The plasma samples were precipitated by acetonitrile to remove the proteins. Separation was carried out on a Shim-pack XR-ODS C,18(2. 0 mm x 100 mm, 2. 2 micromp.m) column using a mobile phase containing methanol-0. 1% formic acid (87:13) using by diazepam as internal standard. Mass spectrometer with electrospray ionization (ESI) operated in the positive ion mode was used for analysis. Total analysis time was 2 min.
  
  
  RESULTS
  The assay was linear in the range 5-5 000 microg L-1 (r =0. 999 3) with recoveries in the range from 69. 0% to 81.2% and satisfied inter-, intra- precision and accuracy. CHR after oral administration is not easy to absorb with double or multimodal peak phenomenon. The t1/2 of CHR after intravenous injection was very short and that of low, medium, and high dosage was (17. 01 +/- 8. 06) , (24. 62 +/- 4. 59), (28. 46+/- 4. 63) min, respectively.
  
  
  CONCLUSIONS
  The developed method was special, rapid, and sensitive for determination of CHR pharmacokinetics. [Key words] UPLC-MS/MS; chrysosplenetin; pharmacokinetics; plasma; rat
authors.affiliation: School of Pharmacy, Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: School of Pharmacy, Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Ji
authors.affiliation: Institute of Clinical Pharmacology, General Hospital of Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Shi-Jie
authors.initials: SJ
authors.lastname: Wei
authors.affiliation: School of Pharmacy, Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Xue-Yan
authors.initials: XY
authors.lastname: Fu
authors.affiliation: School of Pharmacy, Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Meng-Yingi
authors.initials: MY
authors.lastname: Liu
authors.affiliation: School of Pharmacy, Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Xiu-Li
authors.initials: XL
authors.lastname: Wu
mesh_terms: D000818:Animals; D037621:Artemisinins / Q000037:antagonists & inhibitors*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D005419:Flavonoids / Q000097:blood* / Q000494:pharmacology; D008297:Male; D051381:Rats; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D037621:Artemisinins; D005419:Flavonoids; C570121:chrysosplenetin; C031327:artemisinin