title: TDZ pulsing evaluation on the in vitro morphogenesis of peach palm.
abstract: Peach palm (Bactris gasipaes Kunth.) cropping is an excellent alternative to native species exploitation; nevertheless, the problems with seed germination and conventional propagation justify the use of in vitro culturing. Aiming to asses TDZ pulsing effect on B. gasipaes morphogenesis, explants obtained from unarmed microplants were maintained in two treatments, half of them in MS free medium (without growth regulator) and the other half in MS with TDZ (0.36 μM). Both groups were transferred to growth regulator-free MS medium following 14 days of culture. After 84 days of culture, TDZ pulsing increased the growth and development of the shoots, restricted the growth and development of the roots, with no influence on adventitious bud induction or somatic embryogenesis. Furthermore, development of prickles, thickening of roots and chlorotic leaves were noted under TDZ pulsing. Leaf sheath histological analysis showed an epidermal origin and no vascularization of these prickles.
authors.affiliation: Escola Superior de Agricultura "Luiz de Queiroz", Depto. de Ciências Biológicas, PPG em Fisiologia e Bioquímica de Plantas, Universidade de São Paulo, Av. Pádua Dias, n° 11, Caixa Postal 09, 13418-900 Piracicaba, São Paulo Brasil.
authors.forename: Erika Mendes
authors.initials: EM
authors.lastname: Graner
authors.affiliation: Escola Superior de Agricultura "Luiz de Queiroz", Depto. de Ciências Florestais, PPG em Recursos Florestais, Universidade de São Paulo, Av. Pádua Dias, n° 11, Caixa Postal 09, 13418-900 Piracicaba, São Paulo Brasil ; Instituto Nacional de Tecnología Agropecuaria, EEA Concordia, Estación Yuquerí s/n s/n CC 54, Concordia, Entre Ríos Argentina.
authors.forename: Gustavo Pedro Javier
authors.initials: GP
authors.lastname: Oberschelp
authors.affiliation: Engenheiro Florestal, Dr., Departamento de Engenharia Florestal, Universidade Federal de Mato Grosso, Av. Fernando Corrêa da Costa, 2367, Bairro Boa Esperança, 78060-900 Cuiabá, Mato Grosso Brasil.
authors.forename: Gilvano Ebling
authors.initials: GE
authors.lastname: Brondani
authors.affiliation: Escola Superior de Agricultura "Luiz de Queiroz", Depto. de Ciências Biológicas, PPG em Fisiologia e Bioquímica de Plantas, Universidade de São Paulo, Av. Pádua Dias, n° 11, Caixa Postal 09, 13418-900 Piracicaba, São Paulo Brasil.
authors.forename: Katherine Derlene
authors.initials: KD
authors.lastname: Batagin-Piotto
authors.affiliation: InVitroPalm Consultoria, E.D.B. Ltda, Piracicaba, São Paulo Brasil.
authors.forename: Cristina Vieira
authors.initials: CV
authors.lastname: de Almeida
authors.affiliation: Escola Superior de Agricultura "Luiz de Queiroz", Depto. de Ciências Biológicas, PPG em Fisiologia e Bioquímica de Plantas, Universidade de São Paulo, Av. Pádua Dias, n° 11, Caixa Postal 09, 13418-900 Piracicaba, São Paulo Brasil ; Escola Superior de Agricultura "Luiz de Queiroz", Depto. de Ciências Florestais, PPG em Recursos Florestais, Universidade de São Paulo, Av. Pádua Dias, n° 11, Caixa Postal 09, 13418-900 Piracicaba, São Paulo Brasil.
authors.forename: Marcílio
authors.initials: M
authors.lastname: de Almeida
keywords: Bactris gasipaes; Micropropagation; Organogenesis; Prickles; Thidiazuron