title: Molecular characterization and intermolecular interaction of coat protein of Prunus necrotic ringspot virus: implications for virus assembly.
abstract: Coat protein (CP) and RNA3 from Prunus necrotic ringspot virus (PNRSV-rose), the most prevalent virus infecting rose in India, were characterized and regions in the coat protein important for self-interaction, during dimer formation were identified. The sequence analysis of CP and partial RNA 3 revealed that the rose isolate of PNRSV in India belongs to PV-32 group of PNRSV isolates. Apart from the already established group specific features of PV-32 group member's additional group-specific and host specific features were also identified. Presence of methionine at position 90 in the amino acid sequence alignment of PNRSV CP gene (belonging to PV-32 group) was identified as the specific conserved feature for the rose isolates of PNRSV. As protein-protein interaction plays a vital role in the infection process, an attempt was made to identify the portions of PNRSV CP responsible for self-interaction using yeast two-hybrid system. It was found (after analysis of the deletion clones) that the C-terminal region of PNRSV CP (amino acids 153-226) plays a vital role in this interaction during dimer formation. N-terminal of PNRSV CP is previously known to be involved in CP-RNA interactions, but our results also suggested that N-terminal of PNRSV CP represented by amino acids 1-77 also interacts with C-terminal (amino acids 153-226) in yeast two-hybrid system, suggesting its probable involvement in the CP-CP interaction.
authors.affiliation: Plant Virus Lab, Floriculture Division, IHBT (CSIR), Palampur, 176061 HP India ; Faculty of Biotechnology, Shoolini University of Biotechnology and Management Sciences, Solan, 173212 HP India.
authors.forename: Saurabh
authors.initials: S
authors.lastname: Kulshrestha
authors.affiliation: Plant Virus Lab, Floriculture Division, IHBT (CSIR), Palampur, 176061 HP India.
authors.forename: Vipin
authors.initials: V
authors.lastname: Hallan
authors.affiliation: Faculty of Biotechnology, Shoolini University of Biotechnology and Management Sciences, Solan, 173212 HP India.
authors.forename: Anshul
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Faculty of Biotechnology, Shoolini University of Biotechnology and Management Sciences, Solan, 173212 HP India.
authors.forename: Chandrika Attri
authors.initials: CA
authors.lastname: Seth
authors.affiliation: Department of Basic Sciences, Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan, India.
authors.forename: Anjali
authors.initials: A
authors.lastname: Chauhan
authors.affiliation: Plant Virus Lab, Floriculture Division, IHBT (CSIR), Palampur, 176061 HP India.
authors.forename: Aijaz Asghar
authors.initials: AA
authors.lastname: Zaidi
keywords: Coat protein; Interaction; PV-32; Prunus necrotic ringspot virus (PNRSV); Rose