title: Facile fabrication of AIE-based stable cross-linked fluorescent organic nanoparticles for cell imaging.
abstract: An aggregation induced emission dye (RNH2) with two amino end-groups was facilely incorporated into stable cross-linked fluorescent organic nanoparticles via room temperature anhydride ring-opening polymerization and consecutive cross-linking with polyethylene polyamine. Thus obtained RO-OA-PEPA FONs were characterized by a series of techniques including (1)H nuclear magnetic resonance, Fourier transform infrared spectroscopy, UV-vis absorption spectrum, fluorescent spectroscopy and transmission electron microscopy. Biocompatibility evaluation and cell uptake behavior of RO-OA-PEPA FONs were further investigated to explore their potential biomedical application. We demonstrated that such FONs showed high-water dispersibility, strong red fluorescence, stable uniform morphology (100-200 nm) and excellent biocompatibility, making them promising for cell imaging application.
authors.affiliation: Department of Chemistry and Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, China.
authors.forename: Xiqi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Institute of Polymers, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China; Department of Chemistry and Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, China. Electronic address: xiaoyongzhang1980@gmail.com.
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Chemistry and Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Chemistry and Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Hui
authors.affiliation: Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Organic Solids, Laboratory of New Materials, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Meiying
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Chemistry and Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, China. Electronic address: weiyen@tsinghua.edu.cn.
authors.forename: Yen
authors.initials: Y
authors.lastname: Wei
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics*; D045744:Cell Line, Tumor; D002470:Cell Survival; D003432:Cross-Linking Reagents / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics*; D006801:Humans; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011073:Polyamines / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics*; D011095:Polyethylenes / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D011073:Polyamines; D011095:Polyethylenes; C016071:polyethylene polyamine
keywords: Aggregation-induced emission; Anhydride ring-opening polymerization; Cell imaging; Cross-linked fluorescent organic nanoparticles; Polyethylene polyamine