title: One-month strawberry-rich anthocyanin supplementation ameliorates cardiovascular risk, oxidative stress markers and platelet activation in humans.
abstract: Strawberries are an important fruit in the Mediterranean diet because of their high content of essential nutrients and beneficial phytochemicals, which seem to exert beneficial effects in human health. Healthy volunteers were supplemented daily with 500 g of strawberries for 1 month. Plasma lipid profile, circulating and cellular markers of antioxidant status, oxidative stress and platelet function were evaluated at baseline, after 30 days of strawberry consumption and 15 days after the end of the study. A high concentration of vitamin C and anthocyanins was found in the fruits. Strawberry consumption beneficially influenced the lipid profile by significantly reducing total cholesterol, low-density lipoprotein cholesterol and triglycerides levels (-8.78%, -13.72% and -20.80%, respectively; P<.05) compared with baseline period, while high-density lipoprotein cholesterol remained unchanged. Strawberry supplementation also significant decreased serum malondialdehyde, urinary 8-OHdG and isoprostanes levels (-31.40%, -29.67%, -27.90%, respectively; P<.05). All the parameters returned to baseline values after the washout period. A significant increase in plasma total antioxidant capacity measured by both ferric reducing ability of plasma and oxygen radical absorbance capacity assays and vitamin C levels (+24.97%, +41.18%, +41.36%, respectively; P<.05) was observed after strawberry consumption. Moreover, the spontaneous and oxidative hemolysis were significant reduced (-31.7% and -39.03%, respectively; P<.05), compared to the baseline point, which remained stable after the washout period. Finally, strawberry intake significant decrease (P<.05) the number of activated platelets, compared to both baseline and washout values. Strawberries consumption improves plasma lipids profile, biomarkers of antioxidant status, antihemolytic defenses and platelet function in healthy subjects, encouraging further evaluation on a population with higher cardiovascular disease risk.
authors.affiliation: Dipartimento di Science Cliniche Specialistiche ed Odontostomatologiche, Facoltà di Medicina, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: José M
authors.initials: JM
authors.lastname: Alvarez-Suarez
authors.affiliation: Dipartimento di Science Cliniche Specialistiche ed Odontostomatologiche, Facoltà di Medicina, Università Politecnica delle Marche, Ancona, Italy; Dipartimento di Scienze Agrarie, Alimentari ed Ambientali, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Giampieri
authors.affiliation: Research Laboratory, Virgen de la Victoria Clinical Hospital (IMABIS Foundation), Malaga, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Tulipani
authors.affiliation: Neurobiology of Aging Laboratory, INRCA Scientific Technological Area, Ancona, Italy.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Casoli
authors.affiliation: Scientific Direction, INRCA Scientific Technological Area, Ancona, Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Di Stefano
authors.affiliation: Grupo de Investigación en Polifenoles (GIP-USAL), Universidad de Salamanca, Spain.
authors.forename: Ana M
authors.initials: AM
authors.lastname: González-Paramás
authors.affiliation: Grupo de Investigación en Polifenoles (GIP-USAL), Universidad de Salamanca, Spain.
authors.forename: Celestino
authors.initials: C
authors.lastname: Santos-Buelga
authors.affiliation: Analysis Laboratory of INRCA Hospital, Ancona, Italy.
authors.forename: Franco
authors.initials: F
authors.lastname: Busco
authors.affiliation: Instituto de Nutrición y Tecnología de los Alimentos Mataix Verdú, Departamento de Fisiología, Universidad de Granada, Granada, Spain.
authors.forename: Josè L
authors.initials: JL
authors.lastname: Quiles
authors.affiliation: Departamento de Citología e Histología Normal y Patológica, Facultad de Medicina, Universidad de Sevilla, Spain.
authors.forename: Mario D
authors.initials: MD
authors.lastname: Cordero
authors.affiliation: Dipartimento di Scienze Biomediche e Sanità Pubblica, Facoltà di Medicina, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Bompadre
authors.affiliation: Dipartimento di Scienze Agrarie, Alimentari ed Ambientali, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Bruno
authors.initials: B
authors.lastname: Mezzetti
authors.affiliation: Dipartimento di Science Cliniche Specialistiche ed Odontostomatologiche, Facoltà di Medicina, Università Politecnica delle Marche, Ancona, Italy. Electronic address: m.a.battino@univpm.it.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Battino
mesh_terms: D000872:Anthocyanins / Q000008:administration & dosage*; D015415:Biomarkers / Q000378:metabolism*; D002318:Cardiovascular Diseases / Q000378:metabolism*; D031985:Fragaria / Q000737:chemistry*; D006801:Humans; D018384:Oxidative Stress; D015539:Platelet Activation; D012307:Risk Factors
chemical_list: D000872:Anthocyanins; D015415:Biomarkers
keywords: CVD risk; Cholesterol; LDL-C; Platelet activation; Strawberry consumption; Triglycerides