title: An immunoproteomic approach for characterization of the outer membrane proteins of Salmonella Gallinarum.
abstract: Salmonella enterica serovar Gallinarum (SG) is an important pathogen that causes fowl typhoid in chickens. In order to investigate SG outer membrane proteins (OMPs) as potential vaccine candidate proteins, we established a proteomic map and database of antigenic SG-OMPs. A total of 174 spots were detected by 2DE. Twenty-two antigen-reactive spots were identified as nine specific proteins using PMF. OmpA was the most abundant protein among all of the identified OMPs, and it exhibited seven protein species. We conducted Western blot analysis for the SG-OMPs in order to determine which proteins were cross-reactive to the serovars Salmonella Enteritidis, Salmonella Typhimurium, and SG. Our results indicated that OmpA was considered to be an antigenic cross-reactive protein among the three serovars. This study sheds new light on our understanding of cross-protection among Salmonella serovars.
authors.affiliation: College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon, Korea.
authors.forename: Youngjae
authors.initials: Y
authors.lastname: Cho
authors.forename: Jisun
authors.initials: J
authors.lastname: Sun
authors.forename: Jang Hyuck
authors.initials: JH
authors.lastname: Han
authors.forename: Joo Hyun
authors.initials: JH
authors.lastname: Jang
authors.forename: Zheng Wu
authors.initials: ZW
authors.lastname: Kang
authors.forename: Tae-Wook
authors.initials: TW
authors.lastname: Hahn
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D015153:Blotting, Western; D003429:Cross Reactions; D015180:Electrophoresis, Gel, Two-Dimensional; D020543:Proteome / Q000032:analysis* / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D012475:Salmonella / Q000737:chemistry*; D016622:Silver Staining
chemical_list: D001425:Bacterial Outer Membrane Proteins; D020543:Proteome
keywords: Cross-reactivity; Immunoproteomic; Outer membrane proteins; Salmonella Gallinarum