title: Acid tolerance response (ATR) of microbial communities during the enhanced biohydrogen process via cascade acid stress.
abstract: Enhanced biohydrogen production via cascade acid stress on microbial communities, structure patterns of the microbial communities revealed by PLFAs, and the succession of biohydrogen related species against cascade acid stress were all investigated. It was found that hydrogen production could be improved from 48.7 to 79.4mL/gVS after cascade acid stress. In addition, the Gram negative (G(-)) bacteria were found to be more tolerant to organic acids than those of the Gram positive (G(+)) bacteria, regardless of the dominance of G(+) bacteria within the microbial communities. Moreover, Clostridium butyricum, Clostridium aciditolerans and Azospira oryzae, were proved to be enriched, and then might play indispensable roles for the enhanced biohydrogen production after cascade acid stress, as which were responsible for the biohydrogen accumulation, acid tolerance and nitrogen removal, respectively.
authors.affiliation: School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Lin
authors.affiliation: School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xia
authors.affiliation: School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, China. Electronic address: yanqun@jiangnan.edu.cn.
authors.forename: Qun
authors.initials: Q
authors.lastname: Yan
authors.affiliation: School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
authors.affiliation: School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, China.
authors.forename: Mingxing
authors.initials: M
authors.lastname: Zhao
mesh_terms: D000143:Acids / Q000633:toxicity*; D056804:Biofuels; D019149:Bioreactors; D046972:Clostridium butyricum / Q000187:drug effects / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D006859:Hydrogen / Q000378:metabolism*; D013058:Mass Spectrometry; D016133:Polymerase Chain Reaction
chemical_list: D000143:Acids; D056804:Biofuels; D017931:DNA Primers; D006859:Hydrogen
keywords: Acid tolerance response; Biohydrogen; Cascade acid stress; Microbial communities