title: Intra-host evolution of multiple genotypes of hepatitis C virus in a chronically infected patient with HIV along a 13-year follow-up period.
abstract: The intra-host evolutionary process of hepatitis C virus (HCV) was analyzed by phylogenetic and coalescent methodologies in a patient co-infected with HCV-1a, HCV-2a, HCV-3a and human immunodeficiency virus (HIV) along a 13-year period. Direct sequence analysis of the E2 and NS5A regions showed diverse evolutionary dynamics, in agreement with different relationships between these regions and the host factors. The Bayesian Skyline Plot analyses of the E2 sequences (cloned) yielded different intra-host evolutionary patterns for each genotype: a steady state of a "consensus" sequence for HCV-1a; a pattern of lineage splitting and extinction for HCV-2a; and a two-phase (drift/diversification) process for HCV-3a. Each genotype evolving in the same patient and at the same time presents a different pattern apparently modulated by the immune pressure of the host. This study provides useful information for the management of co-infected patients and provides insights into the mechanisms behind the intra-host evolution of HCV.
authors.affiliation: Cátedra de Virología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
authors.forename: A C A
authors.initials: AC
authors.lastname: Culasso
authors.affiliation: Sección Virología, Academia Nacional de Medicina, Buenos Aires, Argentina.
authors.forename: P
authors.initials: P
authors.lastname: Baré
authors.affiliation: Sección Virología, Academia Nacional de Medicina, Buenos Aires, Argentina.
authors.forename: N
authors.initials: N
authors.lastname: Aloisi
authors.affiliation: Sección Virología, Academia Nacional de Medicina, Buenos Aires, Argentina.
authors.forename: M C
authors.initials: MC
authors.lastname: Monzani
authors.affiliation: Departamento de Medicina Interna, Orientación Enfermedades Infecciosas, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina; División VIH/sida, Hospital de Infecciosas F.J. Muñiz, Ciudad Autónoma de Buenos Aires, Argentina; Jefe de Infectología, Fundación Argentina de la Hemofilia, Buenos Aires, Argentina.
authors.forename: M
authors.initials: M
authors.lastname: Corti
authors.affiliation: Cátedra de Virología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina. Electronic address: rcampos@ffyb.uba.ar.
authors.forename: R H
authors.initials: RH
authors.lastname: Campos
mesh_terms: D000328:Adult; D060085:Coinfection / Q000821:virology*; D019143:Evolution, Molecular; D005500:Follow-Up Studies; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D016174:Hepacivirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006526:Hepatitis C / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010802:Phylogeny; D012189:Retrospective Studies; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins
keywords: Co-infection; Coalescene; Evolution; Hepatits C virus; Intra-host