title: MiR-19a is correlated with prognosis and apoptosis of laryngeal squamous cell carcinoma by regulating TIMP-2 expression.
abstract: MiRNAs are small, noncoding RNA molecules that act as posttranscriptional regulators of gene expression and function as important regulators in cancer-related processes. The miR-19a is overexpressed in various cancers and has been causally related to cellular proliferation and growth. To determine whether miR-19a plays a role in laryngeal squamous cell carcinoma (LSCC), we used quantitative real time PCR to detect miR-19a expression in LSCC tissues. We found that miR-19a is overexpressed in LSCC and correlated with neck nodal metastasis, poor differentiation and advanced stage. Statistical analysis suggests that higher level of miR-19a was associated with reduced overall survival. In vitro functional study showed that inhibition of miR-19a by antisense oligonucleotides (ASO) led to apoptosis and reduction of cell proliferation in LSCC cells. Furthermore, growth of LSCC xenograft tumors was significantly suppressed by repeated injection of ASO-miR-19a lentivirus. The TUNEL stain and transmission electron microscopy also detected increased apoptotic cells in ASO-miR-19a treated LSCC xenografts. In addition, both realtime PCR and western blot showed ASO-miR-19a can upregulate TIMP-2 expression and this suggests miR-19a is related with TIMP-2 pathway in LSCC cells. Taken together, these data suggest that miR-19a plays an oncogenic role in the progression of LSCC, and may serve as a biomarker or therapeutic target for patients with LSCC.
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University Harbin, China.
authors.forename: Tian-Yi
authors.initials: TY
authors.lastname: Wu
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, The First Affiliated Hospital, Harbin Medical University Harbin, China.
authors.forename: Tian-Hong
authors.initials: TH
authors.lastname: Zhang
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University Harbin, China.
authors.forename: Ling-Mei
authors.initials: LM
authors.lastname: Qu
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University Harbin, China.
authors.forename: Jia-Peng
authors.initials: JP
authors.lastname: Feng
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University Harbin, China.
authors.forename: Lin-Li
authors.initials: LL
authors.lastname: Tian
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University Harbin, China.
authors.forename: Bing-Hui
authors.initials: BH
authors.lastname: Zhang
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University Harbin, China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Li
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University Harbin, China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Sun
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University Harbin, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D064593:Heterografts; D006801:Humans; D020287:In Situ Nick-End Labeling; D053208:Kaplan-Meier Estimate; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000235:genetics*; D046529:Microscopy, Electron, Transmission; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C549214:MIRN19A microRNA, human; D035683:MicroRNAs; C560823:TIMP2 protein, human; D019716:Tissue Inhibitor of Metalloproteinase-2
keywords: LSCC; TIMP-2; apoptosis; miR-19a