title: Purification of Neurospora crassa alkaline phosphatase without DNAse activity for use in molecular biology.
abstract: Alkaline phosphatase, excreted by Neurospora crassa preg (c) and purified to apparent homogeneity by 7.5% PAGE, did not show DNAase activity and removed the terminal 5'-phosphate group from plasmid Bluescript M13(+) linearized with EcoRI. The preg (c) strain may therefore replace other sources of alkaline phosphatase for use in dephosphorylating linearized plasmidial DNA in cloning experiments.
authors.affiliation: Departamento de Genética, FMRP-USP, Av. Bandeirantes 3900, 14049-900, Ribeirão Preto, SP, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Pereira
authors.forename: H
authors.initials: H
authors.lastname: Pereira
authors.forename: G
authors.initials: G
authors.lastname: Thedei
authors.forename: A
authors.initials: A
authors.lastname: Rossi
authors.forename: N M
authors.initials: NM
authors.lastname: Martinez-Rossi