title: Antibiofilm properties of chemically synthesized silver nanoparticles found against Pseudomonas aeruginosa.
abstract: Nanomedicine is now being introduced as a recent trend in the field of medicine. It has been documented that metal nanoparticles have antimicrobial effects for bacteria, fungi and viruses. Recent advances in technology has revived the use of silver nanoparticles in the medical field; treatment, diagnosis, monitoring and control of disease. It has been used since ancient times for treating wide range of illnesses. Bacterial cells adheres to surfaces and develop structures known as biofilms. These structures are natural survival strategy of the bacteria to invade the host. They are more tolerant to commonly used antimicrobial agents, thus being more difficult to be controlled. This leads to increase in severity of infection. In this study, we have investigated the effect of silver nanoparticles in the formation of biofilm in multidrug resistant strains of Pseudomonas aeruginosa. Observation showed that biofilm formation occurred at bacterial concentration of 10(6) cfu/ml for the sensitive strain of P. aeruginosa while in the resistant strain, the biofilm was evident at bacterial concentration of about 10(3) cfu/ml. The biofilm were then tested against various concentrations of silver nanoparticles to determine the inhibitory effect of the silver nanoparticles. In the sensitive strain, 20 μg/ml of silver nanoparticles inhibited the growth optimally at bacterial concentration of 10(4) cfu/ml with an inhibition rate of 67%. Similarly, silver nanoparticles inhibited the formation of biofilm in the resistant strain at an optimal bacterial concentration of 10(5) cfu/ml with an inhibition rate of 56%. Thus, silver nanoparticles could be used as a potential alternative therapy to reduce severity of disease due to P. aeruginosa infections.
authors.affiliation: Faculty of Medicine, Universiti Teknologi MARA (UiTM), Jalan Hospital, Sg, Buloh, Selangor 47000, Malaysia. navindra87@yahoo.com.
authors.forename: Navindra Kumari
authors.initials: NK
authors.lastname: Palanisamy
authors.forename: Nas
authors.initials: N
authors.lastname: Ferina
authors.forename: Athirah Nur
authors.initials: AN
authors.lastname: Amirulhusni
authors.forename: Zaini
authors.initials: Z
authors.lastname: Mohd-Zain
authors.forename: Jamal
authors.initials: J
authors.lastname: Hussaini
authors.forename: Liew Jian
authors.initials: LJ
authors.lastname: Ping
authors.forename: Rajkumar
authors.initials: R
authors.lastname: Durairaj
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D011552:Pseudomonas Infections / Q000188:drug therapy*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000502:physiology*; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D012834:Silver