title: Duffy antigen / receptor for chemokines correlates with inflammatory reaction in rats with venous hypertension: implication for the pathogenesis of primary chronic venous disease.
abstract: BACKGROUND
  Duffy antigen / receptor for chemokines (DARC) possesses high affinity for several chemokine subgroups of CC and CXC. Although DARC has been shown to play a role in many inflammatory diseases, its effect on chronic venous disease (CVD) remains unidentified. We explored whether the expression of DARC in skin tissue was activated under venous hypertension as well as the relationships between DARC and inflammation.
  
  
  METHODS
  The inflammation in a rat model of venous hypertension caused by a femoral arterial-venous fistula (AVF) was studied. At specified intervals the pressure in the femoral veins was recorded within 42 days. Hindlimb skin specimens were harvested at different time points. The expressions of DARC, interleukin-8 (IL-8), and monocyte chemotactic protein-1 (MCP-1) in skin tissue were examined. Mononuclear cells infiltrated in skin tissue were detected.
  
  
  RESULTS
  Femoral venous pressures in AVF groups increased significantly at different time points (P < 0.01). DARC was expressed in skin tissue and its expression level increased significantly in AVF groups from the 7nd day on and was enhanced in a time-dependent manner within 42 days (P < 0.05). Meanwhile, both MCP-1 and IL-8 had higher levels, accompanied by increased mononuclear cells infiltrating into skin tissue (P < 0.05).
  
  
  CONCLUSIONS
  A rat AVF model which can maintain venous hypertension for at least 42 days is competent for researching the pathogenesis of CVD. DARC, which plays a role in the inflammation of skin tissue under venous hypertension, may become a new molecular target for diagnosis and treatment of CVD at a very early stage.
authors.affiliation: Department of Vascular Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Weibin
authors.initials: W
authors.lastname: Huang
authors.affiliation: Department of Vascular Surgery, First Peoples Hospital of Jining City, Jining, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Qin
authors.affiliation: Department of Vascular Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Lv
authors.affiliation: Department of Vascular Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Haoyv
authors.initials: H
authors.lastname: Deng
authors.affiliation: Department of Vascular Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Vascular Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Jiwei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Vascular Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001166:Arteriovenous Shunt, Surgical; D018932:Chemokine CCL2 / Q000378:metabolism; D002908:Chronic Disease; D004195:Disease Models, Animal; D004375:Duffy Blood-Group System / Q000378:metabolism*; D005263:Femoral Artery / Q000601:surgery; D005268:Femoral Vein / Q000503:physiopathology* / Q000601:surgery; D007249:Inflammation / Q000276:immunology* / Q000503:physiopathology; D016209:Interleukin-8 / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012867:Skin / Q000276:immunology*; D013997:Time Factors; D015854:Up-Regulation; D014652:Vascular Diseases / Q000276:immunology* / Q000503:physiopathology; D014690:Venous Pressure
chemical_list: C586500:Ackr1 protein, rat; C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D004375:Duffy Blood-Group System; D016209:Interleukin-8; D011956:Receptors, Cell Surface
keywords: Venous hypertension; chemokine; duffy antigen / receptor for chemokine; inflammation