title: Genome expression profile analysis of the maize sheath in response to inoculation to R. solani.
abstract: Currently, the molecular regulation mechanisms of disease-resistant involved in maize leaf sheaths infected by banded leaf and sheath blight (BLSB) are poorly known. To gain insight into the transcriptome dynamics that are associated with their disease-resistant, genome-wide gene expression profiling was conducted by Solexa sequencing. More than four million tags were generated from sheath tissues without any leaf or development leaf, including 193,222 and 204,824 clean tags in the two libraries, respectively. Of these, 82,864 (55.4 %) and 91,678 (51.5 %) tags were matched to the reference genes. The most differentially expressed tags with log2 ratio >2 or <-2 (P < 0.001) were further analyzed, representing 1,476 up-regulated and 1,754 down-regulated genes, except for unknown transcripts, which were classified into 11 functional categories. The most enriched categories were those of metabolism, signal transduction and cellular transport. Next, the expression patterns of 12 genes were assessed by quantitative real-time PCR, and it is showed the results were general agreement with the Solexa analysis, although the degree of change was lower in amplitude. In conclusion, we first reveal the complex changes in the transcriptome during the early development of maize sheath infected by BLSB and provide a comprehensive set of data that are essential for understanding its molecular regulation mechanism.
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute of Sichuan Agricultural University, Wenjiang, 611130, Sichuan, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Gao
authors.forename: Zhe
authors.initials: Z
authors.lastname: Chen
authors.forename: Mao
authors.initials: M
authors.lastname: Luo
authors.forename: Hua
authors.initials: H
authors.lastname: Peng
authors.forename: Haijian
authors.initials: H
authors.lastname: Lin
authors.forename: Cheng
authors.initials: C
authors.lastname: Qin
authors.forename: Guangsheng
authors.initials: G
authors.lastname: Yuan
authors.forename: Yaou
authors.initials: Y
authors.lastname: Shen
authors.forename: Haiping
authors.initials: H
authors.lastname: Ding
authors.forename: Maojun
authors.initials: M
authors.lastname: Zhao
authors.forename: Guangtang
authors.initials: G
authors.lastname: Pan
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D001487:Basidiomycota / Q000502:physiology*; D019295:Computational Biology; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D053858:Metabolic Networks and Pathways; D058977:Molecular Sequence Annotation; D015203:Reproducibility of Results; D015398:Signal Transduction; D059467:Transcriptome; D003313:Zea mays / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology*