title: Orientation of Photosystem-I pigments: low temperature linear dichroism spectroscopy of a highly-enriched P700 particle isolated from spinach.
abstract: The linear dichroism of Photosystem I particles containing 10 chlorophylls per P700 has been investigated at 10 K. The particles were oriented by uniaxial squeezing of polyacrylamide gels. The oxidation state of P700 was altered either by incubation of the gels with redox mediators or by low temperature illumination. The QY transitions of the primary electron donor P700, of the remaining unoxidized chlorophyll in P700(+) and of a chlorophyll molecule absorbing at 686 nm, which presumably corresponds to the primary electron acceptor A0, are all preferentially oriented perpendicular to the gel squeezing direction. The QY transition of the chlorophyll forms absorbing at 670 and 675 nm appear tilted at 40 ± 5° from this orientation axis. This orientation of the various chlorophylls is compared to that previously reported for more native Photosystem I particles.
authors.affiliation: Service de Biophysique, Département de Biologie, CEN Saclay, 91191, Gif-sur-Yvette Cedex, France.
authors.forename: J
authors.initials: J
authors.lastname: Breton
authors.forename: I
authors.initials: I
authors.lastname: Ikegami