title: Electrospun tubular scaffold with circumferentially aligned nanofibers for regulating smooth muscle cell growth.
abstract: Simulation for the smooth muscle layer of blood vessel plays a key role in tubular tissue engineering. However, fabrication of biocompatible tube with defined inner nano/micro-structure remains a challenge. Here, we show that a biocompatible polymer tube from poly(l-lactide) (PLLA) and polydimethylsiloxane (PDMS) can be prepared by using electrospinning technique, with assistance of rotating collector and parallel auxiliary electrode. The tube has circumferentially aligned PLLA fibers in the inner surface for cell growth regulation and has a PDMS coating for better compressive property. MTT assay showed the composite PLLA/PDMS tube was suitable for various cells growth. In vitro smooth muscle cells (SMCs) cultured in the tube showed that the aligned PLLA fibers could induce SMCs' orientation, and different expression of α-SMA and OPN genes were observed on the aligned and random PLLA fibers, respectively. The successful fabrication of composite PLLA/PDMS tubular scaffold for regulating smooth muscle cells outgrowth has important implications for tissue-engineered blood vessels.
authors.affiliation: National Research Center of Engineering Plastics, Technical Institute of Physics and Chemistry , Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Wang
authors.forename: Haigang
authors.initials: H
authors.lastname: Shi
authors.forename: Jing
authors.initials: J
authors.lastname: Qiao
authors.forename: Ye
authors.initials: Y
authors.lastname: Tian
authors.forename: Man
authors.initials: M
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lin
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Niu
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000818:Animals; D001483:Base Sequence; D001672:Biocompatible Materials; D002455:Cell Division; D002460:Cell Line; D017931:DNA Primers; D006801:Humans; D051379:Mice; D009130:Muscle, Smooth / Q000166:cytology*; D057139:Nanofibers; D060888:Real-Time Polymerase Chain Reaction; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D017931:DNA Primers