title: Oxoprothracarcin, a novel pyrrolo[1,4]benzodiazepine antibiotic from marine Streptomyces sp. M10946.
abstract: A novel pyrrolo[1,4]benzodiazepine antibiotic, designated oxoprothracarcin (3), was isolated from the marine strain Streptomyces sp. M10946 along with three known secondary metabolites, cyclo(D)-Pro-(D)-Val (1), cyclo(D)-Pro-(D)-Leu (2), and limazepine A (4). The chemical structures of these substances were elucidated by spectroscopic analyses, including 1D- and 2D-NMR and ESI-MS. Antitumor and antibacterial assays indicated that compounds 1-4 weakly inhibit the growth of MDA-MB-231 and A549 cells. The isolation of compound 3 with a high yield (36 mg/10 L) indicated that this marine S. sp. M10946 may provide new lead compounds for structural modification and drug screening.
authors.affiliation: Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Ji'nan, Shandong, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Han
authors.forename: Y Y
authors.initials: YY
authors.lastname: Li
authors.forename: Y
authors.initials: Y
authors.lastname: Shen
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: W J
authors.initials: WJ
authors.lastname: Li
authors.forename: Y M
authors.initials: YM
authors.lastname: Shen
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000903:Antibiotics, Antineoplastic / Q000302:isolation & purification; D001569:Benzodiazepines / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D045744:Cell Line, Tumor; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D021241:Spectrometry, Mass, Electrospray Ionization; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; C000591721:oxoprothracarcin; D001569:Benzodiazepines