title: PDLLA/PRGD/β-TCP conduits build the neurotrophin-rich microenvironment suppressing the oxidative stress and promoting the sciatic nerve regeneration.
abstract: A novel nerve guidance conduit comprising poly{(lactic acid)-co-[(glycolic acid)-alt-(l-lysine)]} (PRGD), poly (d,l-lactic acid) (PDLLA) and β-tricalcium phosphate (β-TCP) was constructed to facilitate the peripheral nerve regeneration. From the comparative study, PDLLA/PRGD/β-TCP conduit achieved the best recovery in regard of the ultrastructure observation and the SFI evaluation. At the first stage of the injury (7 days), the maximum expression augments in ZnSOD (6.4 folds) and GPX4 (6.8 folds) were observed in PDLLA/PRGD/β-TCP group; while striking rise in actin (6.8 folds), tubulin (5.6 folds), and ERM components expressions were observed later (35 days). Meanwhile, compared with PDLLA and PDLLA/PRGD conduits, PDLLA/PRGD/β-TCP conduits achieved the highest local nerve growth factor (NGF) content and an accumulating BDNF content. We speculated that addition of RGD and β-TCP in the composites were the main positive factors to build the microenvironment rich in NGF and BDNF, which help to counteract with the oxidative stress and to boost the cytoskeletal protein expressions. Therefore, PDLLA/PRGD/β-TCP could be promising composites used in peripheral nerve regeneration.
authors.affiliation: Biomedical Materials and Engineering Center, Wuhan University of Technology, Wuhan, 430070, People's Republic of China.
authors.forename: Tong
authors.initials: T
authors.lastname: Qiu
authors.forename: Yixia
authors.initials: Y
authors.lastname: Yin
authors.forename: Binbin
authors.initials: B
authors.lastname: Li
authors.forename: Lijuan
authors.initials: L
authors.lastname: Xie
authors.forename: Qiongjiao
authors.initials: Q
authors.lastname: Yan
authors.forename: Honglian
authors.initials: H
authors.lastname: Dai
authors.forename: Xinyu
authors.initials: X
authors.lastname: Wang
authors.forename: Shipu
authors.initials: S
authors.lastname: Li
mesh_terms: D000818:Animals; D002130:Calcium Phosphates / Q000494:pharmacology*; D060833:Cellular Microenvironment / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D048091:Guided Tissue Regeneration; D019344:Lactic Acid / Q000494:pharmacology*; D008239:Lysine / Q000494:pharmacology*; D020932:Nerve Growth Factor / Q000494:pharmacology*; D009416:Nerve Regeneration / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D011091:Polyesters / Q000494:pharmacology*; D011108:Polymers / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D012584:Sciatic Nerve / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery / Q000648:ultrastructure
chemical_list: D002130:Calcium Phosphates; D011091:Polyesters; D011108:Polymers; D012333:RNA, Messenger; C485817:beta-tricalcium phosphate; C466993:poly(lactic-co-glycolic acid)-grafted-poly(L-lysine); D019344:Lactic Acid; C033616:poly(lactide); D020932:Nerve Growth Factor; D008239:Lysine
keywords: cytoskeletal protein; microenvironment; nerve guidance conduit; oxidative stress; peripheral nerve injury