title: Duodenal mucosal damage is associated with proliferative activity of Brunner's gland hamartoma: a case report.
abstract: BACKGROUND
  Brunner's gland hamartoma is a rare tumor, predominantly found in the fifth to sixth decades of life. Generally, it is a single pedunculated polyp, rarely larger than 5 cm. Asymptomatic cases are found incidentally, but cases with a large polyp tend to have gastrointestinal bleeding and/or obstructive symptoms. Polyp size increases in a time-dependent manner, however, the growth mechanism is unknown. We report a Japanese male case in his mid-twenties with an over 6 cm sized polyp.
  
  
  METHODS
  A 26-year-old man presented black stools and anemia. Endoscopic examination revealed a large pedunculated polyp at gastroduodenal junction. The polyp, subsequently resected by distal gastrectomy, was lobulated with random surface erosions and sized 6.4 × 3 cm. Histological examination revealed that the polyp arose from duodenal mucosa and was composed of hyperplastic Brunner's glands in lobules separated by fibromuscular septa, associated with lymphocytic infiltrate and lymphoid follicles. No evidence of malignancy was found. Thus, the lesion was diagnosed as Brunner's gland hamartoma. Further immunohistochemical studies indicated that gastric foveolar metaplasia is associated with surface epithelium covering upper two thirds of the polyp, showing immunohistochemical positivity for mucin 5 AC (MUC5AC). Below the metaplastic surface epithelium, Brunner's glands had high proliferative activity (MIB-1 labeling index: 7.9%). The similar staining pattern was observed at surface erosive sites (MIB-1 labeling index in Brunner's glands: 9%). On the other hand, surface epithelium in the lower side of the polyp still preserved intestinal nature, containing CDX2-positive nuclei and MUC2-positive goblet cells. Brunner's glands below the surface epithelium with intestinal characteristics showed low proliferative activity (MIB-1 labeling index: 0.77%).
  
  
  CONCLUSIONS
  Proliferative activity of Brunner's glands was high at the sites with surface erosion and also below the epithelium showing gastric foveolar metaplasia. As gastric foveolar metaplasia occurs along with a mucosal repair process in the duodenum, mucosal damages underlay the hamartomatous proliferation of Brunner's glands and eventually resulted in a formation of large polypoid mass in this case.
authors.affiliation: Clinical Laboratory, University of Miyazaki Hospital, 5200 Kihara, Kiyotake, Miyazaki, Miyazaki, 889-1692, Japan. mayumi_akaki@med.miyazaki-u.ac.jp.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Akaki
authors.forename: Shoji
authors.initials: S
authors.lastname: Taniguchi
authors.forename: Kinta
authors.initials: K
authors.lastname: Hatakeyama
authors.forename: Ryoji
authors.initials: R
authors.lastname: Kushima
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kataoka
mesh_terms: D000328:Adult; D002011:Brunner Glands / Q000737:chemistry / Q000473:pathology*; D049109:Cell Proliferation; D004378:Duodenal Diseases / Q000473:pathology*; D006222:Hamartoma / Q000737:chemistry / Q000473:pathology*; D006801:Humans; D007413:Intestinal Mucosa / Q000737:chemistry / Q000473:pathology*; D007417:Intestinal Polyps / Q000737:chemistry / Q000473:pathology*; D019394:Ki-67 Antigen / Q000032:analysis; D008297:Male; D008679:Metaplasia / Q000378:metabolism / Q000473:pathology; D055271:Mucin 5AC / Q000032:analysis
chemical_list: D019394:Ki-67 Antigen; D055271:Mucin 5AC