title: Effects of thiamine and thiamine pyrophosphate on epileptic episode model established with caffeine in rats.
abstract: This study examines the effect of thiamine (TH) and thiamine pyrophosphate (TPP) on epileptic episode model induced in rats with caffeine. Animals were divided into groups and given TH or TPP at doses of 10, 30 or 50mg/kg intraperitoneally. Subsequently, all animal groups were injected intraperitoneally with caffeine at a dose of 300mg/kg. Time of onset of epileptic episode was recorded, and the latent period was calculated in seconds. At the end of the experiment, tGSH and MDA levels and SOD and MPO enzyme activities in extracted brain tissues were measured. Latent period duration in rats in the control group was 134±3.2s, compared to 144±13.9, 147±14.5 and 169±15.1s, respectively, in the TH10, TH30 and TH50 groups and 184±8.54, 197±9.1, 225±8.37s, respectively, in the TPP10, TPP30 and TPP50 groups. Latent period duration was 236±6.7 in the diazepam group. Oxidant products were significantly lower in the TPP10, TPP30, TPP50 and diazepam groups compared to the control group (P<0.05), while SOD activity and tGSH levels were significantly higher (P<0.05). There was no significant difference between the TH10, TH30, TH50 groups and the control group in terms of oxidant and antioxidant levels (P>0.05). In conclusions, TPP, especially at a dose of 50mg/kg, significantly prolonged the latent period from administration of caffeine to time of episode and prevented oxidative damage.
authors.affiliation: Department of Pediatric Neurology, Faculty of Medicine, Ataturk University, Erzurum, Turkey. Electronic address: turan78tr@hotmail.com.
authors.forename: Mehmet Ibrahim
authors.initials: MI
authors.lastname: Turan
authors.affiliation: Department of Pediatric Neurology, Faculty of Medicine, Ataturk University, Erzurum, Turkey.
authors.forename: Huseyin
authors.initials: H
authors.lastname: Tan
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Recep Tayyip Erdogan University, Rize, Turkey.
authors.forename: Nihal
authors.initials: N
authors.lastname: Cetin
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Recep Tayyip Erdogan University, Rize, Turkey.
authors.forename: Halis
authors.initials: H
authors.lastname: Suleyman
authors.affiliation: Department of Pediatric Endocrinology, Research and Educational Hospital, Erzurum, Turkey.
authors.forename: Atilla
authors.initials: A
authors.lastname: Cayir
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002110:Caffeine / Q000633:toxicity*; D000697:Central Nervous System Stimulants / Q000633:toxicity*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004827:Epilepsy / Q000139:chemically induced* / Q000188:drug therapy* / Q000473:pathology; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D013831:Thiamine / Q000627:therapeutic use*; D013835:Thiamine Pyrophosphate / Q000627:therapeutic use*; D014803:Vitamin B Complex / Q000627:therapeutic use*
chemical_list: D000697:Central Nervous System Stimulants; D014803:Vitamin B Complex; D002110:Caffeine; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione; D013835:Thiamine Pyrophosphate; D013831:Thiamine
keywords: Oxidant; Rat; Seizure; Thiamine; Thiamine pyrophosphate