title: Primers-4-Yeast: a comprehensive web tool for planning primers for Saccharomyces cerevisiae.
abstract: The budding yeast Saccharomyces cerevisiae is a key model organism of functional genomics, due to its ease and speed of genetic manipulations. In fact, in this yeast, the requirement for homologous sequences for recombination purposes is so small that 40 base pairs (bp) are sufficient. Hence, an enormous variety of genetic manipulations can be performed by simply planning primers with the correct homology, using a defined set of transformation plasmids. Although designing primers for yeast transformations and for the verification of their correct insertion is a common task in all yeast laboratories, primer planning is usually done manually and a tool that would enable easy, automated primer planning for the yeast research community is still lacking. Here we introduce Primers-4-Yeast, a web tool that allows primers to be designed in batches for S. cerevisiae gene-targeting transformations, and for the validation of correct insertions. This novel tool enables fast, automated, accurate primer planning for large sets of genes, introduces consistency in primer planning and is therefore suggested to serve as a standard in yeast research. Primers-4-Yeast is available at: http://www.weizmann.ac.il/Primers-4-Yeast
authors.affiliation: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Ido
authors.initials: I
authors.lastname: Yofe
authors.forename: Maya
authors.initials: M
authors.lastname: Schuldiner
mesh_terms: D017931:DNA Primers / Q000235:genetics* / Q000378:metabolism; D030561:Databases, Nucleic Acid; D018390:Gene Targeting; D005821:Genetic Techniques / Q000295:instrumentation*; D020407:Internet / Q000295:instrumentation*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D012984:Software; D014170:Transformation, Genetic
chemical_list: D017931:DNA Primers
keywords: DNA; DNA primers: genetics; Saccharomyces cerevisiae; databases; gene deletion; gene targeting; genetic markers; internet; nucleic acid; plasmids; polymerase chain reaction; primers; recombination; software; transformation; user-computer interface; yeast