title: Equipment for large-scale mammalian cell culture.
abstract: This chapter provides information on commonly used equipment in industrial mammalian cell culture, with an emphasis on bioreactors. The actual equipment used in the cell culture process can vary from one company to another, but the main steps remain the same. The process involves expansion of cells in seed train and inoculation train processes followed by cultivation of cells in a production bioreactor. Process and equipment options for each stage of the cell culture process are introduced and examples are provided. Finally, the use of disposables during seed train and cell culture production is discussed.
authors.affiliation: MassBiologics of the University of Massachussets Medical School, 460 Walk Hill Street, Mattapan, MA, 02126, USA, s_ozturk@earthlink.net.
authors.forename: Sadettin S
authors.initials: SS
authors.lastname: Ozturk
mesh_terms: D061249:Batch Cell Culture Techniques / Q000295:instrumentation*; D002469:Cell Separation / Q000295:instrumentation*; D015195:Drug Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D015202:Protein Engineering / Q000295:instrumentation*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000627:therapeutic use*; D012371:Robotics / Q000379:methods*
chemical_list: D011994:Recombinant Proteins