title: Relationship between rate of posterior uveal melanoma flattening following plaque radiotherapy and gene expression profile class of tumor cells.
abstract: OBJECTIVE
  To evaluate the relationship between rate of flattening of posterior uveal melanomas (PUMs) over the first 6 months following I-125 plaque radiotherapy and gene expression profile (GEP) class of tumor cells obtained by fine needle aspiration biopsy (FNAB) prior to treatment.
  
  
  METHODS
  Retrospective analysis of relationship between GEP of PUM cells obtained by FNAB at or prior to treatment and rate of tumor flattening following I-125 plaque radiotherapy. Impact of initial tumor thickness was minimized by pairing cases so baseline tumor thickness in subgroups was matched to within ± 0.5 mm. Paired t-testing compared mean tumor thickness in GEP subgroups at 3- and 6-months post treatment assessments.
  
  
  RESULTS
  Our initial group consisted of 269 patients. Seventy-seven tumors (28.6%) were GEP class 2. Twenty-seven of these were treated by I-125 plaque radiotherapy post-FNAB and returned for post treatment evaluations at 3 and 6 months. A matched GEP class 1 tumor was identified for 25 class 2 cases. Matched tumor pairs ranged in thickness from 2.5 to 11.5 mm at baseline. Mean tumor thickness at baseline in the GEP 1 subgroup was 5.8 and 5.9 mm in the GEP 2 subgroup. Three-months post plaque, mean tumor thickness was 4.5 mm in class 1 cases and 4.6 mm in class 2 cases (paired t = 0.31, P = 0.76). The 6-month post-plaque, mean tumor thickness was 4.0 mm in each subgroup (paired t = 0.25, P = 0.81).
  
  
  CONCLUSIONS
  Our study showed a lack of association between the GEP class and the rate of flattening of posterior uveal melanomas following I-125 plaque radiotherapy of PUMs.
authors.affiliation: Department of Ophthalmology, College of Medicine, University of Cincinnati, Cincinnati, Ohio.
authors.forename: Zélia M
authors.initials: ZM
authors.lastname: Corrêa
authors.forename: James J
authors.initials: JJ
authors.lastname: Augsburger
mesh_terms: D000368:Aged; D044963:Biopsy, Fine-Needle; D001918:Brachytherapy / Q000379:methods*; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008545:Melanoma / Q000175:diagnosis* / Q000235:genetics / Q000532:radiotherapy; D008875:Middle Aged; D012189:Retrospective Studies; D059467:Transcriptome / Q000235:genetics*; D016896:Treatment Outcome; D014463:Ultrasonography; D014602:Uvea / Q000000981:diagnostic imaging / Q000473:pathology*; D014604:Uveal Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000532:radiotherapy; D000098943:Uveal Melanoma
chemical_list: D004273:DNA, Neoplasm
keywords: biopsy; choroidal melanoma; gene expression profile; melanoma; melanoma/plaque therapy; melanoma/regression; needle/methods; ultrasound; uveal neoplasm