title: Optimum conditions for extracting collagen from the tunica albuginea of immunologically castrated pig testes and the functional properties of the isolated collagen.
abstract: This study evaluated alternative methods for extracting collagen from the tunica albuginea of pig testes and characterized the functional properties of the isolated collagen. Using the statistical tools of factorial design (2⁴⁻¹) and a central composite rotatable design (2³), it was concluded that the best conditions were 0.83 mol L⁻¹ acetic acid, 0.24% pepsin and 28 h of hydrolysis to isolate 82.54 g of collagen per 100g of sample. This purified collagen had improved functional properties in relation to bovine skin collagen, including water solubility, water-holding capacity, emulsifying capacity and emulsion stability. These results suggest that isolated collagen from the tunica albuginea can be used in pharmaceutical and food products.
authors.affiliation: Londrina State University, Department of Food Science and Technology, Londrina, Paraná CEP: 86.057-970, Brazil.
authors.forename: Gislaine Silveira
authors.initials: GS
authors.lastname: Simões
authors.affiliation: Meat Technology Center, Institute of Food Technology, Campinas, Sao Paulo CEP: 13.070-178, Brazil.
authors.forename: Expedito Tadeu Facco
authors.initials: ET
authors.lastname: Silveira
authors.affiliation: Meat Technology Center, Institute of Food Technology, Campinas, Sao Paulo CEP: 13.070-178, Brazil.
authors.forename: Simone Raymundo
authors.initials: SR
authors.lastname: de Oliveira
authors.affiliation: Pfizer Animal Health, Brazil.
authors.forename: Evandro
authors.initials: E
authors.lastname: Poleze
authors.affiliation: Pfizer Animal Health, Florham Park, NJ, USA.
authors.forename: Jim R D
authors.initials: JR
authors.lastname: Allison
authors.affiliation: Londrina State University, Department of Food Science and Technology, Londrina, Paraná CEP: 86.057-970, Brazil. Electronic address: elida@uel.br.
authors.forename: Elza Iouko
authors.initials: EI
authors.lastname: Ida
authors.affiliation: Londrina State University, Department of Food Science and Technology, Londrina, Paraná CEP: 86.057-970, Brazil; Federal Technological University of Paraná, Londrina, Paraná CEP: 86.036-370, Brazil.
authors.forename: Massami
authors.initials: M
authors.lastname: Shimokomaki
mesh_terms: D019342:Acetic Acid; D000818:Animals; D002369:Castration / Q000379:methods; D002417:Cattle; D003094:Collagen / Q000737:chemistry / Q000302:isolation & purification*; D004655:Emulsions; D006868:Hydrolysis; D008297:Male; D010434:Pepsin A; D012867:Skin; D012995:Solubility; D013552:Swine; D013737:Testis / Q000737:chemistry*; D014867:Water
chemical_list: D004655:Emulsions; D014867:Water; D003094:Collagen; D010434:Pepsin A; D019342:Acetic Acid
keywords: Central composite rotatable design; Collagen emulsified products; Factorial design; Hydroxyproline