title: Interferon regulatory factor-1 (IRF-1) interacts with regulated in development and DNA damage response 2 (REDD2) in the cytoplasm of mouse bone marrow cells.
abstract: IRF-1 is a critical hematopoietic transcription factor, which regulates cell growth, development of immune cells, immune response, tumor suppression, apoptosis and autophagy in mammalian cells. Protein-protein interactions of IRF-1 in mouse bone marrow cells (BMCs) by GST-IRF-1 pull-down followed by mass spectrometry, coimmunoprecipitation, immunoblotting and colocalization show that regulated in development and DNA damage response 2 (REDD2) is an IRF-1-interacting protein. REDD2 is a highly conserved mammalian regulatory protein of the TSC2/mTOR pathway. It is structurally similar to REDD1 but has a distinct loop region. Cellular IRF-1 and REDD2 complex is present in the cytoplasm of BMCs as distinct speckles in punctate pattern. In vitro interaction of recombinant IRF-1 and REDD2 shows their physical interaction. Taken together, our results suggest that IRF-1 physically interacts with REDD2 in the large cytoplasmic protein complex, which may function as cellular signaling proteins for 'cross-talk' of mTOR and cytokine pathways during regulation of cell growth/proliferation, apoptosis and autophagy of mammalian bone marrow cells during health and disease.
authors.affiliation: Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India.
authors.forename: Manish
authors.initials: M
authors.lastname: Gupta
authors.affiliation: Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India. Electronic address: pcrath@mail.jnu.ac.in.
authors.forename: Pramod C
authors.initials: PC
authors.lastname: Rath
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D001343:Autophagy; D001854:Bone Marrow Cells / Q000166:cytology*; D049109:Cell Proliferation; D003593:Cytoplasm / Q000378:metabolism*; D004268:DNA-Binding Proteins; D006801:Humans; D047468:Immunoprecipitation; D050856:Interferon Regulatory Factor-1 / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D051379:Mice; D062105:Molecular Docking Simulation; D008969:Molecular Sequence Data; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D014157:Transcription Factors; D014404:Tubulin / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D004268:DNA-Binding Proteins; C450979:Ddit4l protein, mouse; D050856:Interferon Regulatory Factor-1; D011506:Proteins; D014157:Transcription Factors; D014404:Tubulin
keywords: Bone marrow cells; Interferon regulatory factor-1; Protein-protein interaction; Regulated in development and DNA damage response 2