title: [Simultaneous determination of five organic acids in Kudiezi injection by HPLC].
abstract: The aim was to develop a high performance liquid chromatography method for simultaneous determination of five organic acids in Kudiezi injection. The Diamonsil C18 column (4.6 mm x 200 mm, 5 microm) was adopted with acetonitrile and water as the mobile phase at a gradient mode program. The flow rate was 1.0 mL min-1 , detection wavelength was 325 nm, and column temperature was 35 degree C. The linear range of monocaffeyltartaric acid, chlorogenic acid, caffeic acid, ferulic acid, and chicoric acid were 0. 64-81.60 (r =0. 999 9),0.09-11. 10 (r =0.999 8) ,0.09-11.30 (r =0. 999 8),0.10-12.80 (r =0.999 9),0.43-55. 50 mg L-1 (r = 0.999 8) , respectively. The average recoveries were 101.8% ,100. 9% ,99. 24% ,99. 83% ,101.9%, respectively, with RSD of less than 2.0%. The developed HPLC method was simple, sensitive and accurate with good repeatability. This work provided helpful information for comprehensive quality control of Kudiezi injection. [Key words] Kudiezi injection; organic acids; content determination; HPLC
authors.affiliation: College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China. lr_8000@163.com
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.affiliation: College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Sun
authors.affiliation: College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhao
authors.affiliation: College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Yu-Bo
authors.initials: YB
authors.lastname: Li
authors.affiliation: College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Zhang
mesh_terms: D002109:Caffeic Acids / Q000032:analysis*; D002726:Chlorogenic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003373:Coumaric Acids / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D011801:Quinic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D013386:Succinates / Q000032:analysis*
chemical_list: D002109:Caffeic Acids; D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; D013386:Succinates; C472707:caffeoylquinic acid; D011801:Quinic Acid; D002726:Chlorogenic Acid; C004999:ferulic acid; C100435:chicoric acid; C040048:caffeic acid