title: Characterization of cDNA encoding resveratrol synthase and accumulation of resveratrol in tartary buckwheat.
abstract: Resveratrol synthase (RS) is the key enzyme for biosynthesis of resveratrol which come from coumaroyl-coenzyme A (CoA) and malonyl-CoA. Here, we report the cloning and characterization of a RS gene and accumulation of resveratrol in tartary buckwheat (Fagopyrum tataricum). FtRS was composed of 1173 bp open reading frame and 390 amino acid residues and had a theoretical molecular weight and isoelectric point value of 43.70 kDa and 6.24, respectively. The FtRS expression levels were examined in sprouts and different organs of two tartary buckwheat cultivars, Hokkai T8 (T8) and Hokkai T10 (T10). FtRS transcript levels and resveratrol contents were higher under the dark condition compared with light condition. The expression levels of different organs of T10 was not observed significant variations compared to different organs of T8. Interestingly, resveratrol was detected in the sprouts developmental stages, but no resveratrol could not detect in any other organs of both T8 and T10. Therefore, we suggest that the resveratrol content in tartary buckwheat sprouts may be attributed mainly to the dark condition. The characterization of FtRS will be helpful for better understanding of the resveratrol biosynthesis in tartary buckwheat.
authors.affiliation: Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Korea.
authors.forename: Yeon Bok
authors.initials: YB
authors.lastname: Kim
authors.affiliation: Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Korea.
authors.forename: Aye Aye
authors.initials: AA
authors.lastname: Thwe
authors.affiliation: Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Korea.
authors.forename: YeJi
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Korea.
authors.forename: Sun Kyung
authors.initials: SK
authors.lastname: Yeo
authors.affiliation: Department of Plant and Environmental New Resources, Kyung Hee University, Yongin, Gyeonggi-do 446-701, Korea.
authors.forename: Chanhui
authors.initials: C
authors.lastname: Lee
authors.affiliation: Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Korea.
authors.forename: Sang Un
authors.initials: SU
authors.lastname: Park
mesh_terms: D000217:Acyltransferases / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D019612:Fagopyrum / Q000378:metabolism*; D000077185:Resveratrol; D013267:Stilbenes / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D013267:Stilbenes; D000217:Acyltransferases; C055200:resveratrol synthase; D000077185:Resveratrol