title: Ion measurements by X-ray microanalysis in unfixed, frozen, hydrated plant cells of species differing in salt tolerance.
abstract: A technique is described for X-ray microanalysis of unfixed, frozen, hydrated higher plant cells using a scanning electron microscope in conjunction with a cryostage. Freezing in liquid N2 is the only preparative step required. Using this method, ion distribution was compared in the roots of Zea mays L. (termed a salt excluder) and Hordeum vulgare L. (which is rather more tolerant), both grown in the presence of NaCl. Distinct differences were observed between the two species in Na, K and Cl distribution. Evidence is presented to support the hypothesis that reabsorption of Na from the xylem sap in the mature regions of the root may occur in salt-sensitive glycophytes such as Z. mays.
authors.affiliation: Botanisches Institut der Technischen Hochschule, Schnittspahnstr. 3-5, D-6100, Darmstadt, Federal Republic of Germany.
authors.forename: A R
authors.initials: AR
authors.lastname: Yeo
authors.forename: A
authors.initials: A
authors.lastname: Läuchli
authors.forename: D
authors.initials: D
authors.lastname: Kramer
authors.forename: J
authors.initials: J
authors.lastname: Gullasch