title: Functional regulation of RNA-induced silencing complex by photoreactive oligonucleotides.
abstract: We developed a novel method for regulation of RISC function by photoreactive oligonucleotides (Ps-Oligo) containing 2'-O-psoralenylmethoxyethyl adenosine (Aps). We observed that inhibitory effects of Ps-Oligos on RISC function were enhanced by UV-irradiation compared with 2'-O-methyl-oligonucleotide without Aps. These results suggest Ps-Oligo inhibited RISC function by cross-linking effect, and we propose that the concept described in this report may be promising and applicable one to regulate the small RNA-mediated post-transcriptional regulation.
authors.affiliation: Department of Biomolecular Engineering, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Matsuyama
authors.affiliation: Department of Biomolecular Engineering, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan. Electronic address: asakoy@kit.ac.jp.
authors.forename: Asako
authors.initials: A
authors.lastname: Yamayoshi
authors.affiliation: Department of Biomolecular Engineering, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Kobori
authors.affiliation: Department of Biomolecular Engineering, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Murakami
mesh_terms: D000241:Adenosine; D003432:Cross-Linking Reagents / Q000737:chemistry; D005363:Ficusin / Q000737:chemistry*; D005786:Gene Expression Regulation / Q000187:drug effects; D006367:HeLa Cells / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D009841:Oligonucleotides / Q000737:chemistry* / Q000493:pharmacokinetics*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D010777:Photochemistry / Q000379:methods; D034743:RNA-Induced Silencing Complex / Q000737:chemistry / Q000235:genetics*; D013816:Thermodynamics; D016963:Thermus thermophilus / Q000235:genetics; D014466:Ultraviolet Rays
chemical_list: D003432:Cross-Linking Reagents; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D034743:RNA-Induced Silencing Complex; D000241:Adenosine; D005363:Ficusin
keywords: Antisense oligonucleotide; Photoreactive; Psoralen; RISC; microRNA