title: Circulating Cell and Plasma microRNA Profiles Differ between Non-ST-Segment and ST-Segment-Elevation Myocardial Infarction.
abstract: BACKGROUND
  Differences in plasma and whole blood expression microRNAs (miRNAs) in patients with an acute coronary syndrome (ACS) have been determined in both in vitro and in vivo studies. Although most circulating miRNAs are located in the cellular components of whole blood, little is known about the miRNA profiles of whole blood subcomponents, including plasma, platelets and leukocytes in patients with myocardial ischemia.
  
  
  METHODS
  Thirteen patients with a ST-segment-elevation (STEMI) or non-ST-segment elevation (NSTEMI) myocardial infarction were identified in the University of Massachusetts Medical Center Emergency Department (ED) or cardiac catheterization laboratory between February and June of 2012. Whole blood was obtained from arterial blood samples at the time of cardiac catheterization and cell-specific miRNA profiling was performed. Expression of 343 miRNAs was quantified from whole blood, plasma, platelets, and peripheral blood mononuclear cells using a high-throughput, quantitative Real-Time polymerase-chain reaction system (qRT-PCR).
  
  
  RESULTS
  MiRNAs associated with STEMI as compared to NSTEMI patients included miR-25-3p, miR-221-3p, and miR-374b-5p. MiRNA 30d-5p was associated with plasma, platelets, and leukocytes in both STEMI and NSTEMI patients; miRNAs 221-3p and 483-5p were correlated with plasma and platelets only in NSTEMI patients.
  
  
  CONCLUSIONS
  Cell-specific miRNA profiles differed between patients with STEMI and NSTEMI. The miRNA distribution is also unique amongst plasma, platelets, and leukocytes in patients with ischemic heart disease or ACS. Our findings suggest unique miRNA profiles among the circulating subcomponents in patients presenting with myocardial ischemia.
authors.affiliation: Department of Emergency Medicine, University of Massachusetts Medical School, Worcester, MA, USA.
authors.forename: Jeanine A
authors.initials: JA
authors.lastname: Ward
authors.affiliation: Cardiac Electrophysiology Section, Cardiovascular Medicine Division, Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA.
authors.forename: Nada
authors.initials: N
authors.lastname: Esa
authors.affiliation: Cardiac Electrophysiology Section, Cardiovascular Medicine Division, Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA.
authors.forename: Rahul
authors.initials: R
authors.lastname: Pidikiti
authors.affiliation: Cardiac Electrophysiology Section, Cardiovascular Medicine Division, Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA.
authors.forename: Jane E
authors.initials: JE
authors.lastname: Freedman
authors.affiliation: Cardiac Electrophysiology Section, Cardiovascular Medicine Division, Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA.
authors.forename: John F
authors.initials: JF
authors.lastname: Keaney
authors.affiliation: Cardiac Electrophysiology Section, Cardiovascular Medicine Division, Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA.
authors.forename: Kahraman
authors.initials: K
authors.lastname: Tanriverdi
authors.affiliation: Cardiac Electrophysiology Section, Cardiovascular Medicine Division, Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA.
authors.forename: Olga
authors.initials: O
authors.lastname: Vitseva
authors.affiliation: Department of Molecular Biology, University of Massachusetts Medical School, Worcester, MA USA.
authors.forename: Victor
authors.initials: V
authors.lastname: Ambros
authors.affiliation: Department of Molecular Biology, University of Massachusetts Medical School, Worcester, MA USA.
authors.forename: Rosalind
authors.initials: R
authors.lastname: Lee
authors.affiliation: Cardiac Electrophysiology Section, Cardiovascular Medicine Division, Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA ; Department of Quantitative Health Sciences, University of Massachusetts, Medical School, Worcester, MA, USA ; Department of Biomedical Engineering, Worcester Polytechnic Institute, Worcester, MA, USA.
authors.forename: David D
authors.initials: DD
authors.lastname: McManus
keywords: Acute coronary syndrome; MicroRNA; Non-ST Elevation Myocardial Infarction (NSTEMI); ST Elevation Myocardial Infarction (STEMI); biomarkers