title: How are cytochrome b561 electron currents controlled by membrane voltage and substrate availability?
abstract: Direct recordings of electron currents mediated by cytochromes b561 (CYB561) are not available yet, despite the importance of these proteins in a variety of physiological functions, including neurotransmitter synthesis and dietary iron uptake. Here, we used the two-electrode voltage-clamp technique applied to Xenopus oocytes to demonstrate, for the first time, the generation of electron currents by a Drosophila member of the CYB561 superfamily named stromal cell-derived receptor 2 (SDR2). This experimental method, along with the theoretical development of a three-state kinetic model, supports the hypothesis that electron donor/acceptor concentrations and transmembrane voltage mutually control SDR2-mediated electron transport activity in a complex but predictable manner.
authors.affiliation: 1 Institute of Biophysics , CNR, Genova, Italy .
authors.forename: Cristiana
authors.initials: C
authors.lastname: Picco
authors.forename: Joachim
authors.initials: J
authors.lastname: Scholz-Starke
authors.forename: Alessia
authors.initials: A
authors.lastname: Naso
authors.forename: Valeria
authors.initials: V
authors.lastname: Preger
authors.forename: Francesca
authors.initials: F
authors.lastname: Sparla
authors.forename: Paolo
authors.initials: P
authors.lastname: Trost
authors.forename: Armando
authors.initials: A
authors.lastname: Carpaneto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003573:Cytochrome b Group / Q000737:chemistry* / Q000378:metabolism; D004330:Drosophila / Q000378:metabolism; D004583:Electrons; D007700:Kinetics; D008566:Membranes / Q000737:chemistry; D010084:Oxidation-Reduction; D018408:Patch-Clamp Techniques; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000378:metabolism; D013379:Substrate Specificity; D014982:Xenopus laevis
chemical_list: D003573:Cytochrome b Group; D011956:Receptors, Cell Surface; C023646:cytochrome b561