title: Labeling of a protein with fluorophores using maleimide derivitization.
abstract: Maleimide derivitization of a protein is an essential tool for putting probes such as fluorescent labels at different sites within a polypeptide chain. This allows one to better understand protein-protein or protein-nucleic acid interactions using various biophysical approaches such as fluorescence anisotropy and fluorescence resonance energy transfer (FRET).
authors.affiliation: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Jagpreet S
authors.initials: JS
authors.lastname: Nanda
authors.affiliation: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: jlorsch@jhmi.edu.
authors.forename: Jon R
authors.initials: JR
authors.lastname: Lorsch
mesh_terms: D002021:Buffers; D005452:Fluoresceins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D012996:Solutions; D013194:Staining and Labeling
chemical_list: D002021:Buffers; D005452:Fluoresceins; D005456:Fluorescent Dyes; D011506:Proteins; D012996:Solutions; C037041:fluorescein 5-maleimide
keywords: Cysteines; Efficiency of labeling calculation; Fluorophores; Maleimide derivitization; Maleimide modification; Protein labeling