title: Effects of fluctuating temperature and silicate supply on the growth, biochemical composition and lipid accumulation of Nitzschia sp.
abstract: Nitzschia sp. (Bacillariophyceae) was grown under temperature and photoperiods mimicking those, typical during summer, spring/fall and winter conditions in the southern United States, and using five silicate (Si) concentrations. In general, higher Si concentrations resulted in higher growth rates in summer and spring/fall conditions and lower organic content. Si-deficient Nitzschia sp. had higher levels of neutral lipid compared to those growing in Si replete media. Under summer conditions, the proportion of saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) was relatively stable compared with spring/fall and winter conditions, and the proportion of polyunsaturated fatty acids (PUFA) was low. In the winter condition, SFA and MUFA showed a gradient of decreasing abundance while PUFA gradients increased with increasing Si concentrations in the medium. Cumulative productivity (optimization of growth and lipid content) would be best in the spring/fall but less so in the other conditions for this strain of Nitzschia sp.
authors.affiliation: Department of Marine Biology, Texas A&M University at Galveston, Galveston, 77553 TX, USA. Electronic address: jiang.yuelu@sz.tsinghua.edu.cn.
authors.forename: Yuelu
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Marine Biology, Texas A&M University at Galveston, Galveston, 77553 TX, USA.
authors.forename: Katherine Starks
authors.initials: KS
authors.lastname: Laverty
authors.affiliation: Texas Agrilife Research, Texas A&M University, Pecos, 79772 TX, USA.
authors.forename: Jola
authors.initials: J
authors.lastname: Brown
authors.affiliation: Department of Marine Biology, Texas A&M University at Galveston, Galveston, 77553 TX, USA.
authors.forename: Marcella
authors.initials: M
authors.lastname: Nunez
authors.affiliation: Texas Agrilife Research, Texas A&M University, Pecos, 79772 TX, USA.
authors.forename: Lou
authors.initials: L
authors.lastname: Brown
authors.affiliation: Texas AgriLife Research, Texas A&M System, Lubbock, 79403 TX, USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Chagoya
authors.affiliation: Texas AgriLife Research, Texas A&M System, Lubbock, 79403 TX, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Burow
authors.affiliation: Department of Marine Biology, Texas A&M University at Galveston, Galveston, 77553 TX, USA; Department of Oceanography, Texas A&M University, College Station, 77843 TX, USA.
authors.forename: Antonietta
authors.initials: A
authors.lastname: Quigg
mesh_terms: D000704:Analysis of Variance; D018533:Biomass; D002244:Carbon / Q000032:analysis; D017377:Diatoms / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D005453:Fluorescence; D050356:Lipid Metabolism / Q000187:drug effects*; D009584:Nitrogen / Q000032:analysis; D010078:Oxazines / Q000378:metabolism; D017640:Silicates / Q000494:pharmacology*; D013696:Temperature
chemical_list: D005227:Fatty Acids; D010078:Oxazines; D017640:Silicates; D002244:Carbon; D009584:Nitrogen; C044808:nile red
keywords: AFDW; C; DW; FAMEs; GC; Growth; Lipid; MS; MUFA; N; NR; Nile Red; Nitzschia sp.; OD; PUFA; SFA; Seasonal variation; Si; Silicate limitation; ash-free dry weight; carbon; dry weight; fatty acid methyl esters; gas chromatography; mass spectrometer; mono-unsaturated fatty acids; nitrogen; optical density; polyunsaturated fatty acids; saturated fatty acids; silicate