title: Continuous culture of Candida utilis: influence of medium nitrogen concentration.
abstract: When Candida utilis was grown in continuous culture, decreasing the concentration of N in the medium affected cell composition, biomass yield, biomass productivity, maximal growth rate and cell morphology. When the dilution rate was low (0.1 h(-1)), reducing N from 1100 to 100 mg/l led to a 40% decrease in RNA content of the cells. Nitrogen-limited growth, which occurred when N<420 mg/l, was associated with significant changes in cell-wall carbohydrates and a significant reduction in the glycogen content of the cells. A set of culture conditions was established which permitted maximal consumption of the main nutrients in the medium and the production of yeast biomass suitable as a source of single-cell protein.
authors.affiliation: Fac. Bioq. Qca y Farmacia, UNT, Cátedra de Microbiologia Industrial, Ayacucho 491, (4.000) S.M., Tucumán, Argentina.
authors.forename: M E
authors.initials: ME
authors.lastname: Lucca
authors.forename: M E
authors.initials: ME
authors.lastname: Romero
authors.forename: D A
authors.initials: DA
authors.lastname: Callieri