title: Flavobacterium lacus sp. nov., isolated from a high-altitude lake, and emended description of Flavobacterium filum.
abstract: Two Gram-stain-negative, non-motile, rod-shaped bacterial strains, designated NP180(T) and NR80, were isolated from water of Nam Co Lake, located in Tibet, China. Growth of strains NP180(T) and NR80 occurred at 4-25 °C and at pH 6.5-10.0 (optima, 15-20 °C and pH 7.5-8.5). The 16S rRNA gene sequence similarity to the phylogenetically closest related strains, Flavobacterium filum EMB 34(T), F. ponti GSW-R14(T) and F. gelidilacus LMG 21477(T), was 95.1, 94.8 and 94.6 %, respectively. The predominant fatty acids were iso-C15 : 0, iso-C15 : 1 G, iso-C17 : 0 3-OH and summed feature 9 (comprising iso-C17 : 1ω9c and/or 10-methyl C16 : 0). The major menaquinone of the two strains was menaquinone 6 (MK-6). Phosphatidylethanolamine, one unidentified aminolipid and one unidentified lipid were the major polar lipids in both strains. The G+C contents of the genomic DNA were 34.9 and 35.1 mol%, respectively, for strains NP180(T) and NR80. DNA-DNA relatedness between strains NP180(T) and NR80 was 99 %, indicating that they belong to the same species. According to phylogenetic inference and phenotypic characteristics, a novel species, Flavobacterium lacus sp. nov., is proposed. The type strain is NP180(T) ( = CGMCC 1.12504(T) = NBRC 109715(T)). An emended description of Flavobacterium filum is also provided.
authors.affiliation: China General Microbiological Culture Collection Center and State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Li
authors.affiliation: China General Microbiological Culture Collection Center and State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Hongcan
authors.initials: H
authors.lastname: Liu
authors.affiliation: China General Microbiological Culture Collection Center and State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Bingda
authors.initials: B
authors.lastname: Sun
authors.affiliation: China General Microbiological Culture Collection Center and State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: China General Microbiological Culture Collection Center and State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Xin
mesh_terms: D000531:Altitude; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D060106:Lakes / Q000382:microbiology*; D016013:Likelihood Functions; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D018609:Tibet; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6