title: MicroRNA-137 down-regulates KIT and inhibits small cell lung cancer cell proliferation.
abstract: MiR-137 expression was examined in parental and drug-resistant cell lines, H446 and H446/CDDP, of small lung cancer (SCLC), and the results showed there was fewer miR-137 expressed in H446/CDDP cells followed by KIT expression emergence. In order to confirm physiological function of these abnormal expressions, H446 and H446/CDDP cells were transfected with miR-137 inhibitor and miR-137 mimics, respectively, after that, miR-137 and KIT expression in two cell lines and drug sensitivity of these cells were evaluated. Results indicated that sensitivity of H446 cells to cisplatin significantly decreased after transfected with miR-137 inhibitor, while miR-137 mimics transfection increased drug sensitivity of H446/CDDP cells and deregulated KIT expression. Our data provided combined evidence that miR-137 was closely related to MDR of SCLC, and interfering of miR-137 expression may attenuate drug resistant of H446/CDDP cells to cisplatin partially through KIT expression regulation. Besides, it has also been proved that KIT might be only one of the downstream molecules of miR-137 that related to SCLC MDR.
authors.affiliation: Department of Respiration, Tangdu Hospital, Fourth Military Medical University, 710038 Xi'an, China; Department of Respiration, Harrison International Peace Hospital, 053000 Hengshui, China.
authors.forename: Peipei
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Respiration, Tangdu Hospital, Fourth Military Medical University, 710038 Xi'an, China.
authors.forename: Lijie
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Physiology, Guangdong Medical College, 523808 Dongguan, China.
authors.forename: Yongjuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Human Respiratory Disease, Xinqiao Hospital, Third Military Medical University, 400037 Chongqing, China.
authors.forename: Fuyun
authors.initials: F
authors.lastname: Ji
authors.affiliation: Department of Respiration, Tangdu Hospital, Fourth Military Medical University, 710038 Xi'an, China. Electronic address: jinfag@fmmu.edu.cn.
authors.forename: Faguang
authors.initials: F
authors.lastname: Jin
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002945:Cisplatin / Q000494:pharmacology; D015536:Down-Regulation / Q000235:genetics; D018432:Drug Resistance, Multiple / Q000235:genetics; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics*; D055752:Small Cell Lung Carcinoma / Q000188:drug therapy / Q000235:genetics*; D014162:Transfection
chemical_list: D000970:Antineoplastic Agents; C545567:MIRN137 microRNA, human; D035683:MicroRNAs; D019009:Proto-Oncogene Proteins c-kit; D002945:Cisplatin
keywords: KIT; MDR; MiR-137; SCLC