title: Proteomic comparison between abdominal and thoracic aortic aneurysms.
abstract: The pathogenesis of abdominal aortic aneurysms (AAAs) and that of thoracic aortic aneurysms (TAAs) is distinct. In this study, to reveal the differences in their biochemical properties, we performed quantitative proteomic analysis of AAAs and TAAs compared with adjacent normal aorta (NA) tissues. The proteomic analysis revealed 176 non-redundant differentially expressed proteins in the AAAs and 189 proteins in the TAAs which were common in at least 5 samples within 7 samples of each. Among the identified proteins, 55 and 68 proteins were unique to the AAAs and TAAs, respectively, whereas 121 proteins were identified in both the AAAs and TAAs. Panther overrepresentation analysis of the unique proteins in the AAAs and TAAs revealed a significant downregulation of the blood coagulation pathway in the AAAs and that of the integrin signaling pathway in the TAAs. On the other hand, Genesis analysis revealed distinct expression patterns of 58 proteins among the 121 proteins. Panther overrepresentation analysis of these 58 proteins revealed that the expression of these proteins in the blood coagulation and the plasminogen activating cascade was decreased in the AAAs, whereas it was increased in the TAAs compared with the NA tissues. On the other hand, the protein expression in the integrin signaling pathway was increased in the AAAs, whereas it was decreased in the TAAs compared with the NA tissues. Thus, the data presented in this study indicate that the proteins that show differential expression patterns in AAAs and TAAs may be involved in the distinct pathogenesis of AAAs and TAAs.
authors.affiliation: Department of Biosignaling and Radioisotope Experiment, Interdisciplinary Center for Science Research, Organization for Research, Shimane University, Izumo 693-8501, Japan.
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Matsumoto
authors.affiliation: Department of Biosignaling and Radioisotope Experiment, Interdisciplinary Center for Science Research, Organization for Research, Shimane University, Izumo 693-8501, Japan.
authors.forename: Kazumi
authors.initials: K
authors.lastname: Satoh
authors.affiliation: Department of Biosignaling and Radioisotope Experiment, Interdisciplinary Center for Science Research, Organization for Research, Shimane University, Izumo 693-8501, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Maniwa
authors.affiliation: Department of Pharmacology, Shimane University School of Medicine, Izumo 693-8501, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: Department of Pharmacology, Shimane University School of Medicine, Izumo 693-8501, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Okunishi
authors.affiliation: Division of Cardiovascular and Thoracic Surgery, Department of Surgery, Shimane University School of Medicine, Izumo 693-8501, Japan.
authors.forename: Teiji
authors.initials: T
authors.lastname: Oda
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001011:Aorta / Q000378:metabolism / Q000473:pathology; D017544:Aortic Aneurysm, Abdominal / Q000378:metabolism* / Q000473:pathology; D017545:Aortic Aneurysm, Thoracic / Q000378:metabolism* / Q000473:pathology; D015153:Blotting, Western; D016000:Cluster Analysis; D005260:Female; D006801:Humans; D007553:Isotope Labeling; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D015398:Signal Transduction; D019063:Tenascin / Q000378:metabolism
chemical_list: D019063:Tenascin