title: Optimization of the bare platinum electrode as an oxygen measurement system in photosynthesis.
abstract: This paper is concerned with the definition of the standard conditions required for optimum operation of the bare platinum electrode with photosynthetic samples. Experimental evidence shows the following: 1) Polarization circuits should have zero resistance; 2) The electrolyte layer between the electrodes should have a conductance higher than 54×10(-6) Ω(-1) per mm(2) of platinum electrode area; 3) The electrodes should be polarized just before taking the measurements. All these facts can be interpreted in terms of phenomena occurring on the electrode: The adsorption of hydrogen on the electrode imposes the need for low resistances in the system, and oxygen consumption by the electrode is minimized by polarizing the electrodes as late as possible. This investigation increases the reliability of the bare platinum electrode and gives a basis for a comparison of the results from different experiments. Demonstrations of the pertinence of these conditions are made in our lab with the algae Dunaliella Tertiolecta.
authors.affiliation: Centre de Recherche en Photobiophysique, Université du Québec à Trois-Rivières, C.P. 500, G9A 5H7, Trois-Rivières, P.Q., Canada.
authors.forename: P C
authors.initials: PC
authors.lastname: Meunier
authors.forename: R
authors.initials: R
authors.lastname: Popovic