title: Poly(3-hydroxybutyrate-co-R-3-hydroxyhexanoate) nanoparticles with polyethylenimine coat as simple, safe, and versatile vehicles for cell targeting: population characteristics, cell uptake, and intracellular trafficking.
abstract: A simple and highly safe poly(3-hydroxybutyrate-co-R-3-hydroxyhexanoate) nanoparticulate delivery system that targets different cell types is developed. A sub-cytotoxic level of polyethylenimine coat mediates universal cell targeting. Internalized nanoparticles traffic along endolysosomal compartments, endoplasmic reticulum and the Golgi complex. Nanoparticles have no detrimental effects on cell morphology and respiration.
authors.affiliation: Centre for Pharmaceutical Nanotechnology and Nanotoxicology, Faculty of Health and Medical Sciences, NanoScience Centre, Faculty of Science, University of Copenhagen, Universitetsparken 2, 2100, Copenhagen, Denmark.
authors.forename: Lin-Ping
authors.initials: LP
authors.lastname: Wu
authors.forename: Danyang
authors.initials: D
authors.lastname: Wang
authors.forename: Ladan
authors.initials: L
authors.lastname: Parhamifar
authors.forename: Arnaldur
authors.initials: A
authors.lastname: Hall
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Chen
authors.forename: Seyed M
authors.initials: SM
authors.lastname: Moghimi
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000737:chemistry*; D001692:Biological Transport; D002208:Caproates / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010316:Particle Size; D011094:Polyethyleneimine / Q000737:chemistry*; D012235:Rhodamines / Q000737:chemistry / Q000378:metabolism; D000094122:Polyhydroxybutyrates
chemical_list: D020155:3-Hydroxybutyric Acid; D002208:Caproates; D011094:Polyethyleneimine; D012235:Rhodamines; C115940:poly(3-hydroxybutyrate-co-3-hydroxyhexanoate); D000094122:Polyhydroxybutyrates; C029773:rhodamine B
keywords: cell targeting; intracellular trafficking; polyethylenimine; polyhydroxyalkanoate; surface coating