title: Purification and properties of β-amylase from Bacillus circulans S31.
abstract: A thermotolerant β-amylase was purified from Bacillus circulans S31 isolated from soil in Hong Kong. The purified enzyme has an M r of 64 kDa and was stable at 50°C and pH 7.0 for 30 min. Its K m for starch was 0.9 mg/ml with a V max of 0.3 mg/min. It was not activated by any metal ion although sulphydrys reagents were inhibitory.
authors.affiliation: , .
authors.forename: H S
authors.initials: HS
authors.lastname: Kwan
authors.forename: K H
authors.initials: KH
authors.lastname: So
authors.forename: K Y
authors.initials: KY
authors.lastname: Chan
authors.forename: S C
authors.initials: SC
authors.lastname: Cheng