title: [Both CK2 catalytic subunits involves in androgen receptor signaling in prostate cancer cells].
abstract: OBJECTIVE
  To explore the roles of different casein kinase 2 (CK2) catalytic subunits in androgen receptor (AR) signaling in prostate cancer cell lines.
  
  
  METHODS
  Prostate cancer cell lines were maintained.Immunofluorescent staining was performed to determine AR nuclear translocation in PC-3/AR cells with R1881 pretreatment and luciferase gene reporter assay used to determine AR transactivation in LNCaP cells. And reverse transcription-polymerase chain reaction (RT-PCR) was employed to evaluate the mRNA level of prostate-specific antigen (PSA).
  
  
  RESULTS
  R1881-induced AR nuclear localization was reduced significantly (P < 0.01).R1881-stimulated ARE-LUC reporter activity in LNCaP cells decreased with reduced level of PSA mRNA, an AR endogenous target (P < 0.05). To confirm the target specificity, the gene-specific siRNAs were used for both CK2α and CK2α' subunits. The results of ARE-LUC assay (38.5 vs 31.4) suggested that both CK2 catalytic subunits were involved in androgen-stimulated AR nuclear translocation and AR-mediated gene expression in prostate cancer cells.
  
  
  CONCLUSIONS
  Both CK2 catalytic subunits are involved in androgen receptor signaling of prostate cancer cells.
authors.affiliation: Department of Urology, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yao
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Gao
authors.forename: Bi-jun
authors.initials: BJ
authors.lastname: Huang
authors.forename: Zai-shang
authors.initials: ZS
authors.lastname: Li
authors.forename: Fang-jian
authors.initials: FJ
authors.lastname: Zhou
authors.forename: Ben-yi
authors.initials: BY
authors.lastname: Li
authors.affiliation: Email: hanhui67@sina.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Han
mesh_terms: D047390:Casein Kinase II / Q000378:metabolism*; D045744:Cell Line, Tumor; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000378:metabolism*; D011944:Receptors, Androgen / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: C410798:AR protein, human; D011944:Receptors, Androgen; D047390:Casein Kinase II