title: Saliva secretion in engrafted mouse bioengineered salivary glands using taste stimulation.
abstract: OBJECTIVE
  The aim of this study was to compare saliva flow and protein composition induced using five basic taste stimulations between natural and bioengineered salivary glands.
  
  
  METHODS
  We developed a mouse saliva secretion model using taste stimulation and analyzed the saliva secretion from natural and bioengineered salivary glands using an assay. The protein components and alpha-amylase in the natural and bioengineered saliva were analyzed by gel electrophoresis and Western blotting.
  
  
  RESULTS
  The salivary flow responses induced by sour (citric acid) and bitter (quinine-HCl) stimuli were significantly high in the natural and bioengineered salivary glands. Although the protein concentrations in the natural and bioengineered saliva induced using five basic taste stimulations were similar, the protein composition and the amylase concentration in the natural saliva after taste stimulation had different profiles. Sympathetic and non-sympathetic nerves were observed around the acini and ducts in the natural and bioengineered salivary glands. However, the frequency of neuropeptide Y-positive sympathetic nerves in the bioengineered gland was relatively high compared to that in the natural gland.
  
  
  CONCLUSIONS
  These results suggest that the signal balance between the sympathetic and parasympathetic components of the efferent nerves in an engrafted bioengineered salivary gland may differ from that in a natural salivary gland.
authors.affiliation: Organ Technologies Inc., Chiyoda-ku, Tokyo 101-0048, Japan; Research Institute for Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan.
authors.forename: Miho
authors.initials: M
authors.lastname: Ogawa
authors.affiliation: Department of Biological Science and Technology, Graduate School of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yamashita
authors.affiliation: Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan.
authors.forename: Minori
authors.initials: M
authors.lastname: Niikura
authors.affiliation: Department of Clinical Pathophysiology, Tokyo Dental College, Chiba-shi, Chiba 261-8502, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Nakajima
authors.affiliation: Research Institute for Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan.
authors.forename: Koh-ei
authors.initials: KE
authors.lastname: Toyoshima
authors.affiliation: Research Institute for Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan.
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Oshima
authors.affiliation: Organ Technologies Inc., Chiyoda-ku, Tokyo 101-0048, Japan; Research Institute for Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan; Department of Biological Science and Technology, Graduate School of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan. Electronic address: t-tsuji@rs.noda.tus.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Tsuji
mesh_terms: D000818:Animals; D051379:Mice; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D009478:Neuropeptide Y; D010812:Physical Stimulation; D011506:Proteins / Q000032:analysis; D012463:Saliva / Q000737:chemistry / Q000378:metabolism*; D012469:Salivary Glands / Q000294:innervation / Q000378:metabolism*; D013564:Sympathetic Nervous System / Q000502:physiology; D013649:Taste / Q000502:physiology*; D000516:alpha-Amylases / Q000032:analysis
chemical_list: D009478:Neuropeptide Y; D011506:Proteins; D000516:alpha-Amylases
keywords: Bioengineered salivary gland; Organ regeneration; Saliva; Taste stimulation