title: Bienzymatic biosensor for rapid detection of aspartame by flow injection analysis.
abstract: A rapid, simple and stable biosensor for aspartame detection was developed. Alcohol oxidase (AOX), carboxyl esterase (CaE) and bovine serum albumin (BSA) were immobilised with glutaraldehyde (GA) onto screen-printed electrodes modified with cobalt-phthalocyanine (CoPC). The biosensor response was fast. The sample throughput using a flow injection analysis (FIA) system was 40 h⁻¹ with an RSD of 2.7%. The detection limits for both batch and FIA measurements were 0.1 µM for methanol and 0.2 µM for aspartame, respectively. The enzymatic biosensor was successfully applied for aspartame determination in different sample matrices/commercial products (liquid and solid samples) without any pre-treatment step prior to measurement.
authors.affiliation: Centre of Bioanalysis, National Institute of Research and Development for Biological Sciences, 296, Splaiul Independentei, Bucharest 060031, Romania. cristinariz2002@yahoo.com.
authors.forename: Maria-Cristina
authors.initials: MC
authors.lastname: Radulescu
authors.affiliation: Centre of Bioanalysis, National Institute of Research and Development for Biological Sciences, 296, Splaiul Independentei, Bucharest 060031, Romania. bucurica@yahoo.com.
authors.forename: Bogdan
authors.initials: B
authors.lastname: Bucur
authors.affiliation: Centre of Bioanalysis, National Institute of Research and Development for Biological Sciences, 296, Splaiul Independentei, Bucharest 060031, Romania. madalina_dondoi@yahoo.com.
authors.forename: Madalina-Petruta
authors.initials: MP
authors.lastname: Bucur
authors.affiliation: Centre of Bioanalysis, National Institute of Research and Development for Biological Sciences, 296, Splaiul Independentei, Bucharest 060031, Romania. rglucian2000@yahoo.com.
authors.forename: Gabriel Lucian
authors.initials: GL
authors.lastname: Radu
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry; D000818:Animals; D001218:Aspartame / Q000737:chemistry / Q000302:isolation & purification*; D015374:Biosensing Techniques; D043182:Carboxylesterase / Q000737:chemistry; D002417:Cattle; D017022:Flow Injection Analysis; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D055767:Immobilized Proteins; D012710:Serum Albumin, Bovine; D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase; D043182:Carboxylesterase; D001218:Aspartame