title: Lung toxicity assessment using bronchoalveolar lavage fluid and pleural lavage fluid cytology by intratracheal treatment in rats.
abstract: Usefulness of bronchoalveolar lavage fluid (BALF) and pleural cavity lavage fluid (PLF) as an experimental material was evaluated for the assessment of pulmonary toxicity of chemicals in rats. From the viewpoint of safety, isoflurane can be used for euthanasia/anesthesia because there was no difference in biological properties of BALF between diethyl ether and isoflurane. Here, we also recognized phosphate buffered saline (PBS) and distilled water equally as a solvent/vehicle for negative control. PLF is also provided as a useful target material as well as BALF for assessing chemical lung toxicity. To evaluate the method, we used zinc chloride as a model chemical and obtained the expected and satisfied results. We may conclude that the intratracheal treatment and combination usage of BALF and PLF as a target material is a good method for assessment of chemical pulmonary (lung and plural cavity) toxicity in rats.
authors.affiliation: Public Interest Incorporated Foundation BioSafety Research Center (BSRC).
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takehara
authors.forename: Maki
authors.initials: M
authors.lastname: Makita
authors.forename: Ryota
authors.initials: R
authors.lastname: Tanaka
authors.forename: Mai
authors.initials: M
authors.lastname: Tsuchiya
authors.forename: Masato
authors.initials: M
authors.lastname: Naya
authors.forename: Makoto
authors.initials: M
authors.lastname: Hayashi
mesh_terms: D000758:Anesthesia; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D002021:Buffers; D002712:Chlorides / Q000008:administration & dosage / Q000633:toxicity*; D004986:Ether; D007322:Instillation, Drug; D007530:Isoflurane; D008168:Lung / Q000187:drug effects*; D008297:Male; D035422:Pleural Cavity / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride; D013047:Specific Pathogen-Free Organisms; D018675:Toxicity Tests / Q000379:methods*; D014132:Trachea; D014867:Water; D017967:Zinc Compounds / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: D002021:Buffers; D002712:Chlorides; D017967:Zinc Compounds; D014867:Water; D004986:Ether; D012965:Sodium Chloride; C016837:zinc chloride; D007530:Isoflurane