title: Antimicrobial, antioxidant and cytotoxic activities of propolis from Melipona orbignyi (Hymenoptera, Apidae).
abstract: Propolis from stingless bees is well known for its biologic properties; however, few studies have demonstrated these effects. Therefore, this study aimed to investigate the chemical composition and antimicrobial, antioxidant and cytotoxic activities of propolis from the stingless bee Melipona orbignyi, found in Mato Grosso do Sul, Brazil. The chemical composition of the ethanol extract of propolis (EEP) indicated the presence of aromatic acids, phenolic compounds, alcohols, terpenes and sugars. The EEP was active against the bacterium Staphylococcus aureus and the fungus Candida albicans. The EEP showed antioxidant activity by scavenging free radicals and inhibiting hemolysis and lipid peroxidation in human erythrocytes incubated with an oxidizing agent. Additionally, EEP promoted cytotoxic activity and primarily necrotic death in K562 erythroleukemia cells. Taken together, these results indicate that propolis from M. orbignyi has therapeutic potential for the treatment and/or prevention of diseases related to microorganism activity, oxidative stress and tumor cell proliferation.
authors.affiliation: School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados Ithaum, Km 12, 79804-970 Dourados, MS, Brazil.
authors.forename: Jaqueline Ferreira
authors.initials: JF
authors.lastname: Campos
authors.affiliation: School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados Ithaum, Km 12, 79804-970 Dourados, MS, Brazil.
authors.forename: Uilson Pereira
authors.initials: UP
authors.lastname: dos Santos
authors.affiliation: School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados Ithaum, Km 12, 79804-970 Dourados, MS, Brazil.
authors.forename: Luis Fernando Benitez
authors.initials: LF
authors.lastname: Macorini
authors.affiliation: University Center of Grande Dourados, Dourados, MS, Brazil.
authors.forename: Adriana Mary Mestriner Felipe
authors.initials: AM
authors.lastname: de Melo
authors.affiliation: School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados Ithaum, Km 12, 79804-970 Dourados, MS, Brazil.
authors.forename: José Benedito Perrella
authors.initials: JB
authors.lastname: Balestieri
authors.affiliation: Department of Biophysics, Federal University of São Paulo, 04023-062 São Paulo, SP, Brazil.
authors.forename: Edgar Julian
authors.initials: EJ
authors.lastname: Paredes-Gamero
authors.affiliation: Course of Chemistry, State University of Mato Grosso do Sul, Rodovia Dourados Ithaum, Km 12, 79804-970 Dourados, MS, Brazil.
authors.forename: Claudia Andrea Lima
authors.initials: CA
authors.lastname: Cardoso
authors.affiliation: School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados Ithaum, Km 12, 79804-970 Dourados, MS, Brazil.
authors.forename: Kely
authors.initials: K
authors.lastname: de Picoli Souza
authors.affiliation: School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados Ithaum, Km 12, 79804-970 Dourados, MS, Brazil. Electronic address: edson.lucas@pq.cnpq.br.
authors.forename: Edson Lucas
authors.initials: EL
authors.lastname: dos Santos
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001516:Bees / Q000737:chemistry*; D004912:Erythrocytes / Q000187:drug effects; D006801:Humans; D020014:K562 Cells; D008826:Microbial Sensitivity Tests; D011429:Propolis / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D000975:Antioxidants; D011429:Propolis
keywords: Anti-tumor activity; Hemolysis; Lipid peroxidation; Necrosis; Oxidative stress; Stingless bee