title: The role of tauroursodeoxycholic acid on adipogenesis of human adipose-derived stem cells by modulation of ER stress.
abstract: Obesity has become a serious public health problem in the developed world. Increased mass of adipose tissue in the obese is due to an increase in both the size (hypertrophy) and number (hyperplasia) of adipocytes. The chemical chaperone tauroursodeoxycholic acid (TUDCA) not only decreases endoplasmic reticulum (ER) stress, but also plays a role as a leptin-sensitizing agent for preadipocytes in mice and humans. In this study, we examine whether TUDCA has an effect on adipogenesis from human adipose-derived stem cells (hASCs). Therefore, the effect of TUDCA on ER stress, lipid accumulation, and adipogenic differentiation from hASCs was investigated using histological staining, reverse-transcriptase polymerase chain reaction (RT-PCR), and western blotting in vitro. It was found that TUDCA treatment of hASCs significantly decreases the representative ER stress marker (glucose-regulated protein 78 kDa (GRP78)), adipogenic markers (peroxisome proliferator-activated receptor gamma (PPARγ) and glycerol-3-phosphate dehydrogenase 1 (GPDH)), and lipid accumulation. Furthermore, we confirmed that TUDCA treatment of hASCs significantly decreased in vivo adipogenic tissue formation and ER stress comparing with PBS treatment of hASCs. The results indicate that TUDCA plays a critical role in adipogenesis from hASCs by modulating ER stress and, therefore, has potential pharmacologic and therapeutic applications as an anti-obesity agent.
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Yatap-Dong, Bundang-Gu, Seongnam-Si, Gyeonggi-Do 463-840, Republic of Korea.
authors.forename: Byung-Hyun
authors.initials: BH
authors.lastname: Cha
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Yatap-Dong, Bundang-Gu, Seongnam-Si, Gyeonggi-Do 463-840, Republic of Korea.
authors.forename: Jin-Su
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Yatap-Dong, Bundang-Gu, Seongnam-Si, Gyeonggi-Do 463-840, Republic of Korea.
authors.forename: Jong Chan
authors.initials: JC
authors.lastname: Ahn
authors.affiliation: Department of Orthopaedics, Bundang CHA Hospital, Sungnam, Republic of Korea.
authors.forename: Hee-Chun
authors.initials: HC
authors.lastname: Kim
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea.
authors.forename: Byung-Soo
authors.initials: BS
authors.lastname: Kim
authors.affiliation: Center for Biomaterials, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul 130-650, Republic of Korea.
authors.forename: Dong Keun
authors.initials: DK
authors.lastname: Han
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Yatap-Dong, Bundang-Gu, Seongnam-Si, Gyeonggi-Do 463-840, Republic of Korea.
authors.forename: Sang Gyu
authors.initials: SG
authors.lastname: Park
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Yatap-Dong, Bundang-Gu, Seongnam-Si, Gyeonggi-Do 463-840, Republic of Korea. Electronic address: soohong@cha.ac.kr.
authors.forename: Soo-Hong
authors.initials: SH
authors.lastname: Lee
mesh_terms: D050156:Adipogenesis / Q000187:drug effects*; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D019070:Cell Lineage / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D000091342:Endoplasmic Reticulum Chaperone BiP; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D050356:Lipid Metabolism / Q000187:drug effects / Q000235:genetics; D008648:Mesoderm / Q000166:cytology; D051379:Mice; D008819:Mice, Nude; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013655:Taurochenodeoxycholic Acid / Q000494:pharmacology*
chemical_list: D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; C000717248:Hspa5 protein, mouse; D013655:Taurochenodeoxycholic Acid; C031655:ursodoxicoltaurine
keywords: Adipogenesis; Chemical chaperone; Endoplasmic reticulum stress; Obesity; Tauroursodeoxycholic acid