title: Differentiation- and apoptosis-inducing activities of rice bran extracts in a human colon cancer cell line.
abstract: Rice bran water extract (RBWE) and ethanol extract (RBEE) at 1.0 mg/ml markedly inhibited the proliferation of LS174T human colon cancer cells. RBEE but not RBWE induced apoptosis. RBWE promoted the production of intestinal mucin (MUC2). Real-time RT-PCR demonstrated that RBWE up-regulated the expression of MUC2 and sucrase-isomaltase complex (a differentiation marker of colon cancer cells), and attenuated that of proliferating cell nuclear antigen at the mRNA level in a dose-dependent manner. These findings suggested that RBWE suppress the proliferation of colon cancer cells by inducting differentiation not apoptosis.
authors.affiliation: Akitameijyo Co., Ltd., 4-23 Daikumachi, Yuzawa Akita, 012-0814 Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Takashima
authors.affiliation: Akitameijyo Co., Ltd., 4-23 Daikumachi, Yuzawa Akita, 012-0814 Japan.
authors.forename: Masanobu
authors.initials: M
authors.lastname: Ohtomo
authors.affiliation: Akitameijyo Co., Ltd., 4-23 Daikumachi, Yuzawa Akita, 012-0814 Japan.
authors.forename: Tsugio
authors.initials: T
authors.lastname: Kikuchi
authors.affiliation: Molecular Biology Laboratory, Faculty of Bioresource Sciences, Akita Prefectural University, Simoshinjo Nakano, Akita, 010-0195 Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Iwashita
authors.affiliation: Molecular Biology Laboratory, Faculty of Bioresource Sciences, Akita Prefectural University, Simoshinjo Nakano, Akita, 010-0195 Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Abe
authors.affiliation: Akita Research Institute for Food & Brewing (ARIF), 4-26 Sanuki, Araya-machi, Akita, 010-1623 Japan.
authors.forename: Keishi
authors.initials: K
authors.lastname: Hata
keywords: Colon cancer differentiation; Intestinal mucin; Proliferating cell nuclear antigen; Rice bran extract