title: Structural characterization of a water-soluble polysaccharide from the fruiting bodies of Agaricus bisporus.
abstract: An edible fungal polysaccharide termed as ABP was obtained by extraction with hot water, and followed successive chromatographic purification using DEAE-Sepharose Fast Flow column and Sephacryl S-300 High-Resolution column. A symmetrical peak was obtained on high-performance size-exclusion chromatography with an average molecular weight of 5.17 × 104 Da, which was named ABP, and its main components were D-glucose and D-mannose. Based on the study of methylation analysis, along with FT-IR, GC, GC-MS, 1D 1H and 13C NMR and 2D NMR (H-HCOSY, TOCSY, HMQC, and NOESY), its chemical structure was featured with a repeating unit (1→6) linking β-D-Glcp as the main backbone with (1→4)-linked α-D-Manp units. The structure of the mainly repeating units of ABP was established as: →6)-β-D-Glcp-(1→4)-α-D-Manp(1→6)-β-D-Glcp-(1→6)-β-D-Glcp-(1→.
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China. hejzgd@163.com.
authors.forename: Jinzhe
authors.initials: J
authors.lastname: He
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China. zhanganqiang@zjut.edu.cn.
authors.forename: Anqiang
authors.initials: A
authors.lastname: Zhang
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China. ruqiaomei@zjut.edu.cn.
authors.forename: Qiaomei
authors.initials: Q
authors.lastname: Ru
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China. ranranbudong@aliyun.com.
authors.forename: Dandan
authors.initials: D
authors.lastname: Dong
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China. sunpeil@163.com.
authors.forename: Peilong
authors.initials: P
authors.lastname: Sun
mesh_terms: D000364:Agaricus / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D017550:Spectroscopy, Fourier Transform Infrared; D014867:Water / Q000737:chemistry
chemical_list: D009005:Monosaccharides; D011134:Polysaccharides; D014867:Water