title: Graphene-gated biochip for the detection of cardiac marker Troponin I.
abstract: We report lithium ion intercalation mediated efficient exfoliation of graphite to form monolithic graphene sheets which have subsequently been investigated for the development of highly sensitive label-free electrochemical detection platform for cardiac biomarker, Troponin I (cTnI). The spectroscopic and morphological analysis demonstrated the formation of defect free graphene sheets which were successfully employed to fabricate an inter-digited microdevice in a drain-source configuration on a silicon biochip. The graphene gated biochip functionalized with anti-cTnI antibodies used in label free detection of cTnI which exhibited an excellent sensitivity in the picogram range (~1 pg mL(-1)) for cTnI without the use of any enzymatic amplification that promises its potential applicability for bio-molecular detection in clinical diagnosis.
authors.affiliation: CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India; CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India.
authors.forename: Satish K
authors.initials: SK
authors.lastname: Tuteja
authors.affiliation: CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India.
authors.lastname: Priyanka
authors.affiliation: CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India.
authors.forename: Vijayender
authors.initials: V
authors.lastname: Bhalla
authors.affiliation: AcSIR-Central Scientific Instruments Organization, Sector 30-C, Chandigarh 160030, India.
authors.forename: Akash
authors.initials: A
authors.lastname: Deep
authors.affiliation: AcSIR-Central Scientific Instruments Organization, Sector 30-C, Chandigarh 160030, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Paul
authors.affiliation: CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India. Electronic address: raman@imtech.res.in.
authors.forename: C Raman
authors.initials: CR
authors.lastname: Suri
mesh_terms: D000906:Antibodies / Q000276:immunology; D000937:Antigen-Antibody Reactions; D015415:Biomarkers / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D056656:Lab-On-A-Chip Devices; D008094:Lithium / Q000737:chemistry; D009206:Myocardium / Q000737:chemistry*; D020107:Troponin T / Q000032:analysis* / Q000276:immunology
chemical_list: D000906:Antibodies; D015415:Biomarkers; D020107:Troponin T; D006108:Graphite; D008094:Lithium
keywords: Biochip; Graphene sheets; Lithium ion intercalation; Troponin I