title: Optimization of enzymatic saccharification of microwave pretreated sugarcane tops through response surface methodology for biofuel.
abstract: The optimization of biomass loading enzyme loading, surfactant concentration and incubation time, using response surface methodology (RSM) and Box Behnken design for enzymatic saccharification of sugarcane tops (SCT) for maximum recovery of fermentable sugars using crude cellulases, resulted in 90.24% saccharification efficiency. Maximum saccharification yield of 0.376 g/g glucose as substrate for ethanol production was observed at optimal conditions of 10% biomass loading (pretreated), 100FPU/g of cellulase loading, 0.04% (w/w) surfactant concentration and 72 h of incubation time.
authors.affiliation: Motilal Nehru National Institute of Technology, Allahabad 211 004, India.
authors.forename: Devendra Prasad
authors.initials: DP
authors.lastname: Maurya
authors.affiliation: Motilal Nehru National Institute of Technology, Allahabad 211 004, India.
authors.forename: Siddharth
authors.initials: S
authors.lastname: Vats
authors.affiliation: Motilal Nehru National Institute of Technology, Allahabad 211 004, India.
authors.forename: Sudheer
authors.initials: S
authors.lastname: Rai
authors.affiliation: Motilal Nehru National Institute of Technology, Allahabad 211 004, India.
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Negi
mesh_terms: D056804:Biofuels; D018533:Biomass; D004798:Enzymes / Q000378:metabolism*; D006868:Hydrolysis; D008872:Microwaves; D031786:Saccharum / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D056804:Biofuels; D004798:Enzymes