title: Scanning electrochemical microscopy for study of aptamer-thrombin interfacial interactions on gold disk microelectrodes.
abstract: A feasibility for the determination of thrombin on gold disk microelectrodes (GDMs) using scanning electrochemical microscopy (SECM) is reported. The assembly process step-by-step of thrombin aptasensor on GDMs is monitored by SECM. SECM analysis reveals the immobilization of thrombin aptamers on GDMs. The interaction between thrombin aptamers and thrombin on GDMs is imaged by SECM with feedback mode using ferrocenemethanol as an electrochemical mediator. The formation of thrombin/thrombin aptamer complex on GDMs results in a decrease in the tip peak current on spatial SECM images. This method is able to linearly and selectively detect thrombin over a linear range from 10(-12) to 10(-5)M with a detection limit of 6.07 fM.
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, 250, Kuo Kuang Road, Taichung 402, Taiwan.
authors.forename: Huei-Yu
authors.initials: HY
authors.lastname: Bai
authors.affiliation: Centro Nacional de Microelectrónica (CNM-IMB), CSIC Campus Universidad Autónoma de Barcelona, Bellaterra 08193, Spain.
authors.forename: F Javier
authors.initials: FJ
authors.lastname: del Campo
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, 250, Kuo Kuang Road, Taichung 402, Taiwan. Electronic address: yctsai@dragon.nchu.edu.tw.
authors.forename: Yu-Chen
authors.initials: YC
authors.lastname: Tsai
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques; D005296:Ferrous Compounds / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D006801:Humans; D007091:Image Processing, Computer-Assisted; D057230:Limit of Detection; D008839:Microelectrodes; D064347:Microscopy, Electrochemical, Scanning; D013917:Thrombin / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D005296:Ferrous Compounds; C071550:ferrocenemethanol; C081676:thrombin aptamer; D006046:Gold; D013917:Thrombin
keywords: Aptamer; Microelectrode; Scanning electrochemical microscope; Thrombin