title: Investigating the response of cuproproteins from oysters (Crassostrea gigas) after waterborne copper exposure by metallomic and proteomic approaches.
abstract: The pacific oyster (Crassostrea gigas), a good model for studying biomineralization and coastal environmental change, was used to study the expression of cuproproteins after waterborne Cu exposure (3 μg Cu L(-1)) similarly to the Arcachon Bay aquatic environment. Cuproproteins in oyster cytosols were analyzed by gel electrophoresis (non-denaturing PAGE and SDS 2-DE), laser ablation inductively coupled plasma mass spectrometry (LA-ICP MS) and electrospray ionization mass spectrometry (ESI-MS). The high molecular weight cuproproteins (identified as extracellular superoxide dismutase [Cu-Zn] (EC Cu/Zn-SOD) and L-ascorbate oxidase) in digestive glands of oysters were differentially expressed after Cu exposure. About 1.7 and 1.2-fold increase of the Cu level in EC Cu/Zn-SOD and L-ascorbate oxidase were, respectively, determined, although EC Cu/Zn-SOD was 1.6-1.9-fold down-regulated in the digestive gland of oysters after Cu exposure. At last, the role of EC Cu/Zn-SOD in protection against Cu exposure was discussed to propose possible mechanisms to better understand Cu homeostasis and/or detoxification mechanisms.
authors.affiliation: CNRS/UPPA, Laboratoire de Chimie Analytique Bio-inorganique et Environnement, UMR 5254, Hélioparc, 2 av. Pr. Angot, F-64053 Pau, France. sandra.mounicou@univ-pau.fr.
authors.forename: Ming
authors.initials: M
authors.lastname: Xu
authors.forename: Hugues
authors.initials: H
authors.lastname: Bijoux
authors.forename: Patrice
authors.initials: P
authors.lastname: Gonzalez
authors.forename: Sandra
authors.initials: S
authors.lastname: Mounicou
mesh_terms: D000818:Animals; D003300:Copper; D049896:Crassostrea / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004064:Digestive System / Q000187:drug effects / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004781:Environmental Exposure / Q000032:analysis*; D005880:Gills / Q000187:drug effects / Q000378:metabolism; D055432:Metabolomics / Q000379:methods*; D008970:Molecular Weight; D063347:Native Polyacrylamide Gel Electrophoresis; D009928:Organ Specificity / Q000187:drug effects; D011506:Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization; D013054:Spectrophotometry, Atomic; D013482:Superoxide Dismutase / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D011506:Proteins; D020543:Proteome; D014874:Water Pollutants, Chemical; D003300:Copper; D013482:Superoxide Dismutase