title: Candida nivariensis isolated from a renal transplant patient with persistent candiduria-Molecular identification using ITS PCR and MALDI-TOF.
abstract: We report on the isolation of Candida nivariensis from a renal transplant patient with persistent candiduria. Biochemical profiling misidentified isolates as Candida glabrata (3/5) and Candida inconspicua (2/5). All isolates produced white colonies on CHROMagar(™) Candida medium. Internal transcribed spacer (ITS) ribosomal gene sequence analysis and MALDI-TOF-MS analysis (Bruker Biotyper(™) 2.0) identified all isolates as C. nivariensis, demonstrating the utility of MALDI-TOF as a rapid, accurate approach for the identification of cryptic Candida species.
authors.affiliation: Department of Microbiology, Royal Free Hospital, Pond Street, London NW3 2QG, United Kingdom.
authors.forename: Rebecca L
authors.initials: RL
authors.lastname: Gorton
authors.affiliation: UCL Centre for Nephrology, Royal Free Hospital, Pond Street, London NW3 2QG, United Kingdom.
authors.forename: Gareth L
authors.initials: GL
authors.lastname: Jones
authors.affiliation: Department of Microbiology, Royal Free Hospital, Pond Street, London NW3 2QG, United Kingdom.
authors.forename: Christopher C
authors.initials: CC
authors.lastname: Kibbler
authors.affiliation: Department of Microbiology, Royal Free Hospital, Pond Street, London NW3 2QG, United Kingdom.
authors.forename: Sophie
authors.initials: S
authors.lastname: Collier
keywords: C. nivariensis; Candiduria; ITS; MALDI-TOF