title: The genome and proteome of Serratia bacteriophage η which forms unstable lysogens.
abstract: BACKGROUND
  Serratia marcescens phage η is a temperate unclassified member of the Siphoviridae which had been reported as containing hypermodified guanine residues.
  
  
  METHODS
  The DNA was characterized by enzymatic digestion followed by HPLC analysis of the nucleoside composition, and by DNA sequencing and proteomic analysis. Its ability to form stable lysogens and integrate was also investigated.
  
  
  RESULTS
  Enzymatic digestion and HPLC analysis revealed phage η DNA did not contain modified bases. The genome sequence of this virus, determined using pyrosequencing, is 42,724 nucleotides in length with a mol% GC of 49.9 and is circularly permuted. Sixty-nine putative CDSs were identified of which 19 encode novel proteins. While seven close genetic relatives were identified, they shared sequence similarity with only genes 40 to 69 of the phage η genome, while gp1 to gp39 shared no conserved relationship. The structural proteome, determined by SDS-PAGE and mass spectrometry, revealed seven unique proteins. This phage forms very unstable lysogens with its host S. marcescens.
authors.forename: Jenna M
authors.initials: JM
authors.lastname: Denyes
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Krell
authors.forename: Richard A
authors.initials: RA
authors.lastname: Manderville
authors.forename: Hans-Wolfgang
authors.initials: HW
authors.lastname: Ackermann
authors.forename: Yi-Min
authors.initials: YM
authors.lastname: She
authors.affiliation: Department of Molecular & Cellular Biology, University of Guelph, Guelph, ON N1G 2W1, Canada. kropinsk@queensu.ca.
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Kropinski
mesh_terms: D001435:Bacteriophages / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D016679:Genome, Viral; D008242:Lysogeny; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D020543:Proteome / Q000032:analysis*; D017422:Sequence Analysis, DNA; D012706:Serratia marcescens / Q000821:virology*; D014764:Viral Proteins / Q000032:analysis*; D016662:Virus Integration
chemical_list: D004279:DNA, Viral; D020543:Proteome; D014764:Viral Proteins