title: Cyclovirus in nasopharyngeal aspirates of Chilean children with respiratory infections.
abstract: Some respiratory tract infections remain unexplained despite extensive testing for common pathogens. Nasopharyngeal aspirates (NPAs) from 120 Chilean infants from Santiago with acute lower respiratory tract infections were analysed by viral metagenomics, revealing the presence of nucleic acids from anelloviruses, adenovirus-associated virus and 12 known respiratory viral pathogens. A single sequence read showed translated protein similarity to cycloviruses. We used inverse PCR to amplify the complete circular ssDNA genome of a novel cyclovirus we named CyCV-ChileNPA1. Closely related variants were detected using PCR in the NPAs of three other affected children that also contained anelloviruses. This report increases the current knowledge of the genetic diversity of cycloviruses whose detection in multiple NPAs may reflect a tropism for human respiratory tissues.
authors.affiliation: Department of Laboratory Medicine, University of California at San Francisco, San Francisco, CA 94118, USA.|Blood Systems Research Institute, San Francisco, CA 94118, USA.
authors.forename: Tung Gia
authors.initials: TG
authors.lastname: Phan
authors.affiliation: Programa de Virología, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
authors.forename: Vivian
authors.initials: V
authors.lastname: Luchsinger
authors.affiliation: Programa de Virología, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
authors.forename: Luis F
authors.initials: LF
authors.lastname: Avendaño
authors.affiliation: Blood Systems Research Institute, San Francisco, CA 94118, USA.
authors.forename: Xutao
authors.initials: X
authors.lastname: Deng
authors.affiliation: Department of Laboratory Medicine, University of California at San Francisco, San Francisco, CA 94118, USA.|Blood Systems Research Institute, San Francisco, CA 94118, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Delwart
mesh_terms: D002677:Chile; D016000:Cluster Analysis; D004266:DNA Virus Infections / Q000821:virology*; D004267:DNA Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D009305:Nasopharynx / Q000821:virology*; D010802:Phylogeny; D012141:Respiratory Tract Infections / Q000821:virology*; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral