title: In vitro effect of amphotericin B on Candida albicans, Candida glabrata and Candida parapsilosis biofilm formation.
abstract: Candida spp. biofilm is considered highly resistant to conventional antifungals. The aim of this study was to investigate the in vitro effect of amphotericin B on Candida spp. biofilms at different stages of maturation. We investigated the activity of amphotericin B against 78 clinical isolates of Candida spp., representing three species, growing as planktonic and sessile cells, by a widely accepted broth microdilution method. The in vitro effect on sessile cell viability was evaluated by MTT reduction assay. All examined strains were susceptible to amphotericin B when grown as free-living cells. At the early stages of biofilm maturation 96.7-100.0 % strains, depending on species, displayed amphotericin B sessile minimal inhibitory concentration (SMIC) ≤ 1 μg/mL. Mature Candida spp. biofilm of 32.1-90.0 % strains displayed amphotericin B SMIC ≤ 1 μg/mL. Based on these results, amphotericin B displays species- and strain-depending activity against Candida spp. biofilms.
authors.affiliation: Department of Microbiology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, 9 Maria Skłodowska-Curie Street, 85-094, Bydgoszcz, Poland, malgorzata_szabelska@wp.pl.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Prażyńska
authors.forename: Eugenia
authors.initials: E
authors.lastname: Gospodarek
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects* / Q000302:isolation & purification / Q000472:pathogenicity; D041221:Candida glabrata / Q000187:drug effects* / Q000302:isolation & purification / Q000472:pathogenicity; D002448:Cell Adhesion / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests; D013045:Species Specificity
chemical_list: D000935:Antifungal Agents; D000666:Amphotericin B