title: Complete mitochondrial genome of the stonefly Cryptoperla stilifera Sivec (Plecoptera: Peltoperlidae) and the phylogeny of Polyneopteran insects.
abstract: We present the complete mitogenome of a stonefly, Cryptoperla stilifera Sivec (Plecoptera; Peltoperlidae). The mitogenome was a circular molecule consisting of 15,633 nucleotides, 37 genes and a A+T-rich region. C. stilifera mitogenome was similar to Pteronarcys princeps mitogenome (Plecoptera; Pteronarcyidae). All transfer RNA genes (tRNAs) had typical cloverleaf secondary structures except for trnSer (AGN), where the stem-loop structure of the dihydrouridine (DHU) arm was missing. The A+T-rich region of C. stilifera had two stem-loops and each had two interlink. Three conserved sequence blocks (CSBs) were present in the A+T-rich regions of C. stilifera, Peltoperla tarteri and Peltoperla arcuata. Moreover, many polynucleotide stretches (Poly N, N=A, T and C) in the A+T-rich region of C. stilifera Phylogenetic relationships of Polyneopteran species were constructed based on the nucleotide sequences of 13 protein coding genes (PCGs). Both maximum likelihood (ML) and Bayesian inference (BI) analyses supported Grylloblattodea as the sister group to Plecoptera+Dermaptera and Embiidina and Phasmatodea as sister groups.
authors.affiliation: School of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, China; School of Biological Science and Technology, Yangzhou University, Yangzhou 225009, China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Wu
authors.affiliation: School of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, China.
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Ji
authors.affiliation: School of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, China.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Yu
authors.affiliation: School of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, China. Electronic address: yzdu@yzu.edu.cn.
authors.forename: Yu-Zhou
authors.initials: YZ
authors.lastname: Du
mesh_terms: D000818:Animals; D001483:Base Sequence; D001499:Bayes Theorem; D003062:Codon; D054629:Genome, Mitochondrial; D007313:Insecta / Q000235:genetics*; D019901:Locus Control Region; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D012313:RNA / Q000737:chemistry; D012343:RNA, Transfer / Q000737:chemistry / Q000235:genetics
chemical_list: D003062:Codon; D012313:RNA; D012343:RNA, Transfer
keywords: ATP6 and ATP8; BI; Base pair (s); Bayesian inference; C. stilifera; COI-III; CR; CSBs; Conserved sequence blocks; Control region; CytB; Gene encoding for cytochrome oxidase b; Genes encoding for ATP synthase subunits 6 and 8; Genes encoding for NADH dehydrogenase subunits 1–6 and 4L; Genes encoding for cytochrome c oxidase subunits I–III; Large and small subunits of ribosomal RNA; ML; Mitochondrial DNA; Mitochondrial genomics; Molecular phylogeny; ND1-6 and ND4L; PCG(s); PCR; Plecoptera; Polymerase chain reaction; Polyneoptera; Protein coding gene(s); SSU rRNA; bp; lrRNA and srRNA; maximum likelihood; mtDNA; nuclear small subunit ribosomal RNA; tRNA(s); transfer RNA (genes)