title: Cannabinoid type-1 receptors in the paraventricular nucleus of the hypothalamus inhibit stimulated food intake.
abstract: Cannabinoid receptor type 1 (CB1)-dependent signaling in the brain is known to modulate food intake. Recent evidence has actually shown that CB1 can both inhibit and stimulate food intake in fasting/refeeding conditions, depending on the specific neuronal circuits involved. However, the exact brain sites where this bimodal control is exerted and the underlying neurobiological mechanisms are not fully understood yet. Using pharmacological and electrophysiological approaches, we show that local CB1 blockade in the paraventricular nucleus of the hypothalamus (PVN) increases fasting-induced hyperphagia in rats. Furthermore, local CB1 blockade in the PVN also increases the orexigenic effect of the gut hormone ghrelin in animals fed ad libitum. At the electrophysiological level, CB1 blockade in slices containing the PVN potentiates the decrease of the activity of PVN neurons induced by long-term application of ghrelin. Hence, the PVN is (one of) the site(s) where signals associated with the body's energy status determine the direction of the effects of endocannabinoid signaling on food intake.
authors.affiliation: INSERM U862 NeuroCentre Magendie, Bordeaux, France; Université de Bordeaux, Bordeaux, France. Electronic address: edgar.soria@inserm.fr.
authors.forename: E
authors.initials: E
authors.lastname: Soria-Gómez
authors.affiliation: INSERM U862 NeuroCentre Magendie, Bordeaux, France; Université de Bordeaux, Bordeaux, France.
authors.forename: F
authors.initials: F
authors.lastname: Massa
authors.affiliation: INSERM U862 NeuroCentre Magendie, Bordeaux, France; Université de Bordeaux, Bordeaux, France.
authors.forename: L
authors.initials: L
authors.lastname: Bellocchio
authors.affiliation: Grupo de Cannabinoides, Facultad de Medicina, UNAM, México City, Mexico.
authors.forename: P E
authors.initials: PE
authors.lastname: Rueda-Orozco
authors.affiliation: INSERM U862 NeuroCentre Magendie, Bordeaux, France; Université de Bordeaux, Bordeaux, France.
authors.forename: P
authors.initials: P
authors.lastname: Ciofi
authors.affiliation: INSERM U862 NeuroCentre Magendie, Bordeaux, France; Université de Bordeaux, Bordeaux, France.
authors.forename: D
authors.initials: D
authors.lastname: Cota
authors.affiliation: INSERM U862 NeuroCentre Magendie, Bordeaux, France; Université de Bordeaux, Bordeaux, France.
authors.forename: S H R
authors.initials: SH
authors.lastname: Oliet
authors.affiliation: Grupo de Cannabinoides, Facultad de Medicina, UNAM, México City, Mexico.
authors.forename: O
authors.initials: O
authors.lastname: Prospéro-García
authors.affiliation: INSERM U862 NeuroCentre Magendie, Bordeaux, France; Université de Bordeaux, Bordeaux, France. Electronic address: giovanni.marsicano@inserm.fr.
authors.forename: G
authors.initials: G
authors.lastname: Marsicano
mesh_terms: D000818:Animals; D063387:Cannabinoid Receptor Antagonists / Q000494:pharmacology; D054439:Ghrelin / Q000494:pharmacology; D006963:Hyperphagia / Q000503:physiopathology*; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D010286:Paraventricular Hypothalamic Nucleus / Q000187:drug effects / Q000502:physiology*; D010880:Piperidines / Q000494:pharmacology; D011720:Pyrazoles / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D043884:Receptor, Cannabinoid, CB1 / Q000037:antagonists & inhibitors / Q000502:physiology*
chemical_list: D063387:Cannabinoid Receptor Antagonists; D054439:Ghrelin; D010880:Piperidines; D011720:Pyrazoles; D043884:Receptor, Cannabinoid, CB1; C103505:AM 251
keywords: CB1; fasting; food intake; ghrelin; hypothalamus; rats