title: An immunological method for quantitative determination of photosynthetic fructose-1,6-bisphosphatase in leaf crude extracts.
abstract: An immunological method for quantitative determination of photosynthetic fructose-1,6-bisphosphatase in crude extracts of leaves is proposed. It is based on the ELISA technique, and offers two modifications. A non-competitive technique has a higher sensitivity and is the right option for samples of low fructose-1,6-bisphosphatase content. However, this method is not sufficiently specific when the total protein is higher than 5 μg/cm(3); so, despite its lower sensitivity, in these circumstances a competitive technique is more suitable. Thus photosynthetic fructose-1,6-bisphosphatase can be measured without interferences from the gluconeogenic cytosolic enzyme of the photosynthetic cell or from a non-specific phosphatase present in the chloroplast.
authors.affiliation: Unidad de Bioquímica Vegetal, Estación Experimental del Zaidín (CSIC), Profesor Albareda 1, 18008, Granada, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Hermoso
authors.forename: A
authors.initials: A
authors.lastname: Chueca
authors.forename: J J
authors.initials: JJ
authors.lastname: Lazaro
authors.forename: J
authors.initials: J
authors.lastname: Lopez-Gorge