title: Seeing is believing: dynamic changes in renal epithelial autophagy during injury and repair.
abstract: Ischemic injury to the kidneys is a prevalent clinical problem, contributing importantly to chronic kidney disease. Yet, underlying molecular mechanisms are elusive. To address the possible role of autophagy, we engineered a novel strain of mice harboring a ubiquitously expressed CAG-RFP-EGFP-LC3 transgene. Using this tool, we examined the post-ischemic kidney and detailed the dynamics of renal tubular epithelial autophagy. In addition, we defined the role of MTOR in the resolution of autophagy during epithelial survival and kidney repair.
authors.affiliation: Department of Pediatrics; Columbia University College of Physicians and Surgeons; and.
authors.forename: Fangming
authors.initials: F
authors.lastname: Lin
authors.affiliation: Department of Internal Medicine; University of Texas Southwestern Medical Center; Dallas, TX USA.
authors.forename: Zhao V
authors.initials: ZV
authors.lastname: Wang
authors.affiliation: Department of Internal Medicine; University of Texas Southwestern Medical Center; Dallas, TX USA; Department of Molecular Biology; University of Texas Southwestern Medical Center; Dallas, TX USA.
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Hill
mesh_terms: D000818:Animals; D001343:Autophagy / Q000235:genetics / Q000502:physiology*; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000166:cytology*; D007668:Kidney / Q000293:injuries / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D015427:Reperfusion Injury
keywords: dymanics; ischemic injury; renal epithelial cells; renal repair; tandem fluorescence