title: [Advances of consolidated bioprocessing based on recombinant strategy].
abstract: Lignocellulosic biomass represents an abundant, low-cost and renewable source of potentially fermentable sugars. It is acandidate besides petroleum as feedstock for fuel and chemical production. Recent researches on utilizing lignocellulosicsas feedstock boost development of numerous-promising processes for a variety of fuels and chemicals, such as biodiesel, biohydrogen and ethanol. However, high cost in depolymerization is a primary obstacle preventing the use of lignocellulosic biomass as feedstock. Consolidated bioprocessing (CBP), refers to the bioprocess without any exogenous cellulolyotic enzymes added, converting the lignocellulosic material into biochemicals directly, which could potentially avoid the cost of the dedicated enzyme generation step by incorporating enzyme-generating, biomass-degrading and bioproduct-producing capabilities into a single organism through genetic engineering. There are two CBP strategies, native strategy and recombinant strategy. We mainly introduce the recombinant strategy, including its principle, the two responding styles, the contributions of synthetic biology and metabolic engineering and the future challenges.
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Zongbao
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Meina
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Xueming
authors.initials: X
authors.lastname: Zhao
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D056804:Biofuels; D001709:Biotechnology / Q000379:methods; D000431:Ethanol / Q000378:metabolism; D005658:Fungi / Q000235:genetics / Q000378:metabolism; D006867:Hydrolases / Q000096:biosynthesis / Q000235:genetics; D007218:Industrial Microbiology / Q000379:methods; D008031:Lignin / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D056804:Biofuels; D011994:Recombinant Proteins; C036909:lignocellulose; D000431:Ethanol; D008031:Lignin; D006867:Hydrolases