title: Production of thermotolerant β-amylase byBacillus circulans.
abstract: An isolate from a Hong Kong soil sample which produced β-amylase was identified as a thermotolerant strain ofBacillus circulans with a growth range of 35 to 55δC. The β-amylase was stable at 45°C for 30 min but lost half of its activity after 30 min at 50°C. Maximum specific activity of β-amylase (36.2 units/mg protein) in the culture broth was detected after 36 h of cultivation at 45°C in a medium containing soluble starch, beef extract, coconut water and inorganic salts.
authors.affiliation: Department of Biology, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong.
authors.forename: H S
authors.initials: HS
authors.lastname: Kwan
authors.forename: K H
authors.initials: KH
authors.lastname: So
authors.forename: K Y
authors.initials: KY
authors.lastname: Chan
authors.forename: S C
authors.initials: SC
authors.lastname: Cheng