title: Functional analysis of a dietary recombinant fatty acid binding protein 10 (FABP10) on the Epinephelus coioides in response to acute low temperature challenge.
abstract: The effect of Ec-FABP10 (Epinephelus coiodes-FABP10) on growth performance, enzyme activity, respiratory burst, MDA level, ATP content, immune-related gene expression of juvenile orange-spotted grouper (E. coioides). The commercial diet supplemented with FABP10 protein was feed to orange-spotted grouper for six weeks. No significant difference was observed in the specific growth rates, while the survival rate in the FABP10 additive group was significantly higher. After the feeding trial, the groupers were exposed to acute low temperature challenge. The decreased level of respiratory burst activity was observed in the FABP10 additive group after the exposure to the acute low temperature stress, while the blood cell count increased significantly at 15 °C and a significant increase of ATP content was observed at 10 °C. Higher enzymatic activities of CAT and SOD were observed at 20 °C and 15 °C, respectively. Meanwhile, the lower level of MDA was observed after the exposure to acute low temperature challenge by comparing with the controls. Further transcript expression analyses of FABP10, SOD2, GPX4, HSPA4 and LIPC in liver by quantitative real-time PCR demonstrated that the up-regulated transcript expression of FABP10, SOD2, HSPA4 and LIPC was observed in FABP10 additive group at 15 °C, while the transcript expression of GPX4 increased significantly at 20 °C. Western blotting analysis confirmed that FABP10 protein expression strongly increased at 15 ± 0.5 °C in FABP10 additive group. These results showed that FABP10 additive diet could moderate the metabolic and immune abilities mainly via ROS pathway in the orange-spotted grouper.
authors.affiliation: Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, PR China.
authors.forename: Sheng-Wei
authors.initials: SW
authors.lastname: Luo
authors.affiliation: Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, PR China.
authors.forename: Luo
authors.initials: L
authors.lastname: Cai
authors.affiliation: Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, PR China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, PR China. Electronic address: wangwn@scnu.edu.cn.
authors.forename: Wei-Na
authors.initials: WN
authors.lastname: Wang
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D001492:Bass / Q000254:growth & development / Q000276:immunology / Q000502:physiology*; D003080:Cold Temperature; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements / Q000032:analysis; D050556:Fatty Acid-Binding Proteins / Q000008:administration & dosage / Q000235:genetics* / Q000378:metabolism*; D029941:Fish Proteins / Q000008:administration & dosage / Q000235:genetics* / Q000378:metabolism*; D005398:Fisheries; D020869:Gene Expression Profiling / Q000662:veterinary; D005786:Gene Expression Regulation; D006403:Hematologic Tests / Q000662:veterinary; D007113:Immunity, Innate; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D013312:Stress, Physiological
chemical_list: D050556:Fatty Acid-Binding Proteins; D029941:Fish Proteins
keywords: Acute low temperature stress; Dietary recombinant Fatty acid binding protein 10; Epinephelus coioides; Oxidative stress