title: [The topically hemostatic effects of batroxobin on the carotid arteries adventitia removal rabbit].
abstract: OBJECTIVE
  To observe the topically hemostatic effects of batroxobin (BX) in different concentrations on the carotid arteries adventitia removal rabbit.
  
  
  METHODS
  18 rabbits were removed vascular adventitia by collagenase digestion and mechanical dissection, causing capillary hemorrhage. Then all of them were randomly divided into 6 groups: blank control, negative control group, 0.5, 1, 2 and 4 kU/L (U/ml) BX group. The hemostatic time and bleeding volume were observed to compare the hemostatic effect of each group. Haematoxylin-eosin, Masson staining and immunohistochemistry were performed to assure adventitia removed.
  
  
  RESULTS
  It was feasible to remove vascular adventitia with collagenase digestion and mechanical dissection. The hemostatic time and bleeding volume were significantly different (P < 0.05) from 0.5 U/ml BX group [(97 ± 20)s,(0.102 ± 0.013)g/cm(2)] of the negative control group[(143 ± 33)s,(0.130 ± 0.023) g/cm(2)]. With the increase of BX concentration, there was a significant difference (P < 0.05) between 2 U/ml BX group (32 ± 13,0.056 ± 0.015) and 1 U/ml BX group (32 ± 13,0.056 ± 0.015), but there was no statistical significance (P > 0.05) between 2 U/ml BX group and 4 U/ml BX group (28 ± 14,0.053 ± 0.012). Thus, the best topical hemostatic concentration of BX was 2 U/ml.
  
  
  CONCLUSIONS
  The topical hemostatic effect of batroxobin is reliable in small area of blood oozing.
authors.affiliation: Department of General Surgery, Second Hospital of Anhui Medical University, Hefei 230601, China.
authors.forename: Yuan-cheng
authors.initials: YC
authors.lastname: Hu
authors.affiliation: Department of General Surgery, Second Hospital of Anhui Medical University, Hefei 230601, China. Email: wshy63@sina.com.
authors.forename: Sheng-yun
authors.initials: SY
authors.lastname: Wan
authors.affiliation: Department of General Surgery, Second Hospital of Anhui Medical University, Hefei 230601, China.
authors.forename: Dan-dan
authors.initials: DD
authors.lastname: Qin
authors.affiliation: Department of General Surgery, Second Hospital of Anhui Medical University, Hefei 230601, China.
authors.forename: Yan-qing
authors.initials: YQ
authors.lastname: Zhan
authors.affiliation: Department of General Surgery, Second Hospital of Anhui Medical University, Hefei 230601, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Ding
mesh_terms: D063194:Adventitia / Q000601:surgery*; D000818:Animals; D012103:Batroxobin / Q000008:administration & dosage / Q000494:pharmacology*; D002339:Carotid Arteries / Q000601:surgery*; D005260:Female; D006489:Hemostatic Techniques; D006490:Hemostatics / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D011817:Rabbits
chemical_list: D006490:Hemostatics; D012103:Batroxobin