title: Development of amperometric L-tyrosine sensor based on Fe-doped hydroxyapatite nanoparticles.
abstract: A novel biosensor based on Fe-doped hydroxyapatite (Fe-HA) nanoparticles and tyrosinase has been developed for the detection of L-tyrosine. Nanostructured Fe-HA was synthesized by a simple microwave irradiation method, and its phase formation, morphology and magnetic property were examined by powder X-ray diffraction (XRD), transmission electron microscopy (TEM) and vibrating sample magnetometer (VSM). Electrochemical performance of the nano Fe-HA/tyrosinase modified glassy carbon electrode (GCE) for detection of L-tyrosine was investigated by cyclic voltammetry (CV) and amperometric methods. The fabricated biosensor exhibited a linear response to L-tyrosine over a wide concentration range of 1.0×10(-7) to 1.0×10(-5) M with a detection limit of 245 nM at pH 7.0. In addition, the fabricated sensor showed an excellent selectivity, good reproducibility, long-term stability and anti-interference towards the determination of L-tyrosine.
authors.affiliation: Department of Bioelectronics and Biosensors, Alagappa University, Karaikudi-630003, Tamil Nadu, India.
authors.forename: P
authors.initials: P
authors.lastname: Kanchana
authors.affiliation: Department of Bioelectronics and Biosensors, Alagappa University, Karaikudi-630003, Tamil Nadu, India.
authors.forename: N
authors.initials: N
authors.lastname: Lavanya
authors.affiliation: Department of Bioelectronics and Biosensors, Alagappa University, Karaikudi-630003, Tamil Nadu, India. Electronic address: Sekar2025@gmail.com.
authors.forename: C
authors.initials: C
authors.lastname: Sekar
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation; D003217:Conductometry / Q000295:instrumentation*; D017886:Durapatite / Q000737:chemistry*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007501:Iron / Q000737:chemistry*; D014442:Monophenol Monooxygenase / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation; D014443:Tyrosine / Q000032:analysis* / Q000737:chemistry
chemical_list: D014443:Tyrosine; D017886:Durapatite; D007501:Iron; D014442:Monophenol Monooxygenase
keywords: Fe-doped hydroxyapatite; biosensor; direct electrochemistry; l-tyrosine; tyrosinase