title: Micropropagation of Ajuga bracteosa, a medicinal herb.
abstract: For conservation and genetic transformation, a successful in vitro micropropagation protocol for Ajuga bracteosa, a medicinal herb has been established for the first time. MS medium supplemented with IAA (2 mg/L) and BA (5 mg/L) induced 100 % shoot regeneration with an average of 41.4 shoots of 8.4 cm per culture. Excised in vitro shoots when transferred to MS + IBA (0.5 mg/L) produced 20 roots/shoot of 20.2 cm average length in 100 % cultures. Of the three explants, leaf, petiole and root, leaf displayed quickest response followed by petiole while root was the slowest. Hardening of plantlets was achieved with 82 % survival. The hardened plants were maintained in pots with garden soil under controlled (Temp. 25 ± 2 °C) conditions. RAPD exhibited genetic fidelity with 100 % monomorphism in regenerants.
authors.affiliation: Department of Biotechnology, Jamia Hamdard, New Delhi, India 110062.
authors.forename: Shivanee
authors.initials: S
authors.lastname: Kaul
authors.affiliation: Department of Botany, University of Delhi, New Delhi, India 110 007.
authors.forename: Sandip
authors.initials: S
authors.lastname: Das
authors.affiliation: Department of Biotechnology, Faculty of Science, Hamdard University, New Delhi, 110062 India.
authors.forename: P S
authors.initials: PS
authors.lastname: Srivastava
keywords: Ajuga bracteosa; Medicinal plant; Micropropagation; RAPD