title: A stable synergistic microbial consortium for simultaneous azo dye removal and bioelectricity generation.
abstract: Microbial species coexist in natural or engineered settings, where they encounter extensive competition and cooperation. Interactions occurring through metabolite exchange or direct contact might be important in establishment of functional biodegradation consortium. Understanding these interactions can facilitate manipulation of selected communities and exploitation of their capacity for specific industrial applications. Here, a simple dual-species consortium (Pseudomonas putida and Shewanella oneidensis) was established for examining simultaneous Congo red bioremediation in planktonic culture and power generation in anode biofilms. Compared to mono-species cultures, co-cultures generated higher current densities and could concurrently degrade Congo red over 72h. Disabling the large secreted adhesion protein, LapA, of P. putida greatly enhanced S. oneidensis biofilm formation on the anode, which increased power generation in co-cultures. This demonstrates simultaneous control of specific planktonic and biofilm communities could be effective in manipulating microbial communities for targeted applications.
authors.affiliation: Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore 637551, Singapore; School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore.
authors.forename: Victor Bochuan
authors.initials: VB
authors.lastname: Wang
authors.affiliation: Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore 637551, Singapore; Graduate School of Integrative Sciences and Engineering, National University of Singapore, Singapore 117543, Singapore.
authors.forename: Song-Lin
authors.initials: SL
authors.lastname: Chua
authors.affiliation: Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore 637551, Singapore; Interdisciplinary Graduate School, Nanyang Technological University, Singapore 637551, Singapore.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Cai
authors.affiliation: Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore 637551, Singapore; Interdisciplinary Graduate School, Nanyang Technological University, Singapore 637551, Singapore.
authors.forename: Krishnakumar
authors.initials: K
authors.lastname: Sivakumar
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore.
authors.forename: Qichun
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore 637551, Singapore; School of Biotechnology and Biomolecular Sciences and Centre for Marine Bio-Innovation, The University of New South Wales, Sydney, NSW 2052, Australia.
authors.forename: Staffan
authors.initials: S
authors.lastname: Kjelleberg
authors.affiliation: Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore 637551, Singapore; School of Civil and Environmental Engineering, Nanyang Technological University, Singapore 639798, Singapore. Electronic address: bincao@ntu.edu.sg.
authors.forename: Bin
authors.initials: B
authors.lastname: Cao
authors.affiliation: Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore 637551, Singapore; School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore.
authors.forename: Say Chye Joachim
authors.initials: SC
authors.lastname: Loo
authors.affiliation: Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore 637551, Singapore; School of Biological Sciences, Nanyang Technological University, Singapore 637551, Singapore. Electronic address: yangliang@ntu.edu.sg.
authors.forename: Liang
authors.initials: L
authors.lastname: Yang
mesh_terms: D001674:Bioelectric Energy Sources / Q000382:microbiology*; D018441:Biofilms; D019149:Bioreactors; D003224:Congo Red / Q000302:isolation & purification / Q000378:metabolism*; D005453:Fluorescence; D007218:Industrial Microbiology / Q000379:methods*; D016958:Pseudomonas putida / Q000378:metabolism; D020592:Shewanella / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*
chemical_list: D014874:Water Pollutants, Chemical; D003224:Congo Red
keywords: Bioelectrochemical systems; Bioremediation; Pseudomonas putida; Shewanella oneidensis