title: The ββα fold of zinc finger proteins as a "natural" protecting group. Chemoselective synthesis of a DNA-binding zinc finger derivative.
abstract: We report the selective modification of cysteine residues engineered in peptides that have two additional cysteine residues as part of a Cys2His2 zinc finger motif. The chemoselective modification is achieved, thanks to the protecting effect exerted by the zinc cation upon coordination with the native cysteines and histidines of the zinc-finger fold. The strategy allows a straightforward synthesis of DNA binding zinc finger constructs.
authors.affiliation: Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS), Departamento de Química Orgánica. Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain. joseluis.mascarenas@usc.es.
authors.forename: Jéssica
authors.initials: J
authors.lastname: Rodríguez
authors.forename: Jesús
authors.initials: J
authors.lastname: Mosquera
authors.forename: Olalla
authors.initials: O
authors.lastname: Vázquez
authors.forename: M Eugenio
authors.initials: ME
authors.lastname: Vázquez
authors.forename: José L
authors.initials: JL
authors.lastname: Mascareñas
mesh_terms: D001665:Binding Sites; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry*; D015394:Molecular Structure; D015202:Protein Engineering; D017510:Protein Folding; D016335:Zinc Fingers
chemical_list: D006639:Histidine; D004247:DNA; D003545:Cysteine