title: Purification of the Immunogenic Fractions and Determination of Toxicity in Mesobuthus eupeus (Scorpionida: Buthidae) Venom.
abstract: BACKGROUND
  Scorpions stings are a health problem in many parts of the world. Mesobuthus eupeus (Buthidae) is the most prevalent species in the Middle East and Central Asia. Definition of toxicogenic and immunogenic characteristics of the venom is necessary to produce antidote. In this study, the noted properties of M. eupeus venom were evaluated.
  
  
  METHODS
  Venom was obtained by milking M. eupeus scorpions for lyophilization. Toxicity was determined after injecting the venom to albino mice and calculating LD50. Polyclonal antibodies against M. eupeus venom were obtained from immunized rabbits. The CH-Sepharose 4B column was used for isolating the specific antibodies. 10 mg of the affinity-purified antibodies were conjugated with a CH-Sepharose 4B column and M. eupeus venom was applied to the column. The bound fragments were eluted using hydrogen chloride (pH: 2.5). Crude venom and affinity-purified fractions of the venom were analyzed by SDS-PAGE technique.
  
  
  RESULTS
  Lethal dose (LD) was 8.75, 11.5 and 4.5 mg/kg for IP, SC and IV respectively. The LD50 of M. eupeus venom was 6.95 mg/kg. The crude venom had 12 detectable bands with molecular weights of 140, 70, 50, 33, 30, 27, 22, 18, 14, 10 kDa and two bands less than 5 kDa. The affinity-purified venom presented eight bands. The 27 kDa band was clearly sharper than other bands but 70, 18, 10 and one of the less than 5 kDa bands were not observed.
  
  
  CONCLUSIONS
  Contrary to popular belief, which know scorpion venom as non-immunogenic composition, the current study was shown that the most fractions of the M. eupeus are immunogenic.
authors.affiliation: Health Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Khoobdel
authors.affiliation: Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: Taghi
authors.initials: T
authors.lastname: Zahraei-Salehi
authors.affiliation: Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: Bahar
authors.initials: B
authors.lastname: Nayeri-Fasaei
authors.affiliation: Health Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Khosravi
authors.affiliation: Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Omidian
authors.affiliation: Razi Vaccine and Serum Research Institute-Karaj Branch, Karaj, Iran.
authors.forename: Mohammad Hassan
authors.initials: MH
authors.lastname: Motedayen
authors.affiliation: Razi Vaccine and Serum Research Institute-Karaj Branch, Karaj, Iran.
authors.forename: Abolfazal
authors.initials: A
authors.lastname: Akbari
keywords: Immunogenic; Mesobuthus eupeus; Scorpion; Toxicogenic; Venom