title: Evaluation of (131)I-pentamidine for scintigraphy of experimentally Leishmania tropica-infected hamsters.
abstract: We aimed to assess the ability of (131)I-Pentamidine scintigraphy to detect the lesions of Leishmania tropica infection. An experimental model of cutaneous leishmaniasis was developed. The presence of cutaneous leishmaniasis was confirmed. Pentamidine was radioiodinated with (131)I. The radiolabeled pentamidine was validated by the requisite quality control tests to check its radiolabeling efficiency, in vitro stability. (131)I-Pentamidine (activity: 18.5 MBq/100 µl) was injected intracardiacally into infected hamsters. Static whole body images of the hamsters were acquired under the gamma camera at 5 and 30 min, 2, 6 and 24 h following the administration. On the scintigrams, anatomically adjusted regions of interest (ROIs) were drawn over the right feet (target) and left feet (not-target) and various organs. Accumulation of (131)I-Pentamidine at sites of infection is expressed as the target to non-target (T/NT) ratio. The results T/NT ratio decreased with time. In concluding the (131)I-Pentamidine has poor sensitivity in detection of L. tropica infection.
authors.affiliation: Department of Parasitology, School of Medicine, Dokuz Eylul University , Inciralti, Izmir , Turkey .
authors.forename: Tonay
authors.initials: T
authors.lastname: Inceboz
authors.forename: Fatma Yurt
authors.initials: FY
authors.lastname: Lambrecht
authors.forename: Mine Şencan
authors.initials: MŞ
authors.lastname: Eren
authors.forename: Nogay
authors.initials: N
authors.lastname: Girginkardeşler
authors.forename: Recep
authors.initials: R
authors.lastname: Bekiş
authors.forename: Osman
authors.initials: O
authors.lastname: Yilmaz
authors.forename: Özge
authors.initials: Ö
authors.lastname: Er
authors.forename: Ahmet
authors.initials: A
authors.lastname: Özbilgin
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D007457:Iodine Radioisotopes; D007895:Leishmania tropica / Q000187:drug effects / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000000981:diagnostic imaging* / Q000469:parasitology; D008297:Male; D008647:Mesocricetus; D010419:Pentamidine / Q000737:chemistry / Q000493:pharmacokinetics; D011877:Radionuclide Imaging; D014018:Tissue Distribution; D014344:Trypanocidal Agents / Q000737:chemistry / Q000493:pharmacokinetics; D051598:Whole Body Imaging
chemical_list: D007457:Iodine Radioisotopes; D014344:Trypanocidal Agents; D010419:Pentamidine
keywords: 131I-Pentamidine scintigraphy; Amastigote; Leishmania tropica; Leishmaniasis hamster; promastigote