title: Unraveling the cytotoxic potential of Temozolomide loaded into PLGA nanoparticles.
abstract: BACKGROUND
  Nanotechnology has received great attention since a decade for the treatment of different varieties of cancer. However, there is a limited data available on the cytotoxic potential of Temozolomide (TMZ) formulations. In the current research work, an attempt has been made to understand the anti-metastatic effect of the drug after loading into PLGA nanoparticles against C6 glioma cells.Nanoparticles were prepared using solvent diffusion method and were characterized for size and morphology. Diffusion of the drug from the nanoparticles was studied by dialysis method. The designed nanoparticles were also assessed for cellular uptake using confocal microscopy and flow cytometry.
  
  
  RESULTS
  PLGA nanoparticles caused a sustained release of the drug and showed a higher cellular uptake. The drug formulations also affected the cellular proliferation and motility.
  
  
  CONCLUSIONS
  PLGA coated nanoparticles prolong the activity of the loaded drug while retaining the anti-metastatic activity.
authors.forename: Darshana S
authors.initials: DS
authors.lastname: Jain
authors.affiliation: Department of Pharmaceutics, C,U, Shah College of Pharmacy, SNDT Women's University, Juhu Tara Road, Santacruz (West), Mumbai 400 049, India. rajani.athawale@gmail.com.
authors.forename: Rajani B
authors.initials: RB
authors.lastname: Athawale
authors.forename: Amrita N
authors.initials: AN
authors.lastname: Bajaj
authors.forename: Shruti S
authors.initials: SS
authors.lastname: Shrikhande
authors.forename: Peeyush N
authors.initials: PN
authors.lastname: Goel
authors.forename: Yuvraj
authors.initials: Y
authors.lastname: Nikam
authors.forename: Rajiv P
authors.initials: RP
authors.lastname: Gude