title: In vitro chloramphenicol detection in a Haemophilus influenza model using an aptamer-polymer based electrochemical biosensor.
abstract: A sensitive and selective electrochemical biosensor is developed for the determination of chloramphenicol (CAP) exploring its direct electron transfer processes in in-vitro model and pharmaceutical samples. This biosensor exploits a selective binding of CAP with aptamer, immobilized onto the poly-(4-amino-3-hydroxynapthalene sulfonic acid) (p-AHNSA) modified edge plane pyrolytic graphite. The electrochemical reduction of CAP was observed in a well-defined peak. A quartz crystal microbalance (QCM) study is performed to confirm the interaction between the polymer film and the aptamer. Cyclic voltammetry (CV) and square wave voltammetry (SWV) were used to detect CAP. The in-vitro CAP detection is performed using the bacterial strain of Haemophilus influenza. A significant accumulation of CAP by the drug sensitive H. influenza strain is observed for the first time in this study using a biosensor. Various parameters affecting the CAP detection in standard solution and in in vitro detection are optimized. The detection of CAP is linear in the range of 0.1-2500 nM with the detection limit and sensitivity of 0.02 nM and 0.102 µA/nM, respectively. CAP is also detected in the presence of other common antibiotics and proteins present in the real sample matrix, and negligible interference is observed.
authors.affiliation: Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India.
authors.forename: Saurabh K
authors.initials: SK
authors.lastname: Yadav
authors.affiliation: Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India.
authors.forename: Bharati
authors.initials: B
authors.lastname: Agrawal
authors.affiliation: Amity Institute of Biotechnology, Amity University Uttar Pradesh, Gautam Buddha Nagar, Noida 201303, Uttar Pradesh, India.
authors.forename: Pranjal
authors.initials: P
authors.lastname: Chandra
authors.affiliation: Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India. Electronic address: rngcyfcy@iitr.ac.in.
authors.forename: Rajendra N
authors.initials: RN
authors.lastname: Goyal
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis / Q000494:pharmacology; D052157:Aptamers, Nucleotide / Q000235:genetics*; D001681:Biological Assay / Q000295:instrumentation; D015374:Biosensing Techniques / Q000295:instrumentation; D002701:Chloramphenicol / Q000032:analysis* / Q000494:pharmacology*; D003217:Conductometry / Q000295:instrumentation*; D004305:Dose-Response Relationship, Drug; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006193:Haemophilus influenzae / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology; D007118:Immunoassay / Q000295:instrumentation; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000900:Anti-Bacterial Agents; D052157:Aptamers, Nucleotide; D011108:Polymers; D002701:Chloramphenicol
keywords: Aptamer; Chloramphenicol; Haemophilus influenza; In vitro detection; Voltammetry