title: Evidence for electrogenic proton extrusion by subepidermal cells of Lemna paucicostata 6746.
abstract: The cell potential of Lemna paucicostata 6746 was measured between the vacuole and the external solution. The potential in the dark (-202 mV) could be depolarized with 0.1 mM dicyclohexyl carbodiimide (DCCD) or 1 mM arsenate to-81 mV. The hyperpolarization above the latter value is therefore attributed to an ATP-dependent process. The cell potential showed a significant dependence upon the pH of the external solution. The change in the potential induced by a jump in pH between two certain values, was reversible and independent of the mode of performing the pH change (stepwise or at once). The DCCD-or arsenate-depolarized potential did not respond to external pH changes. A 0.1 mM ammonium chloride solution depolarized the cell potential reversibly to-83 mV. This potential-change could be greatly reduced by simultaneous addition of 5 mM Na isobutyrate. The pH sensitivity of the cell potential is ascribed to changes in the rate of proton extrusion upon altering the proton gradient across the plasmalemma. The effects of ammonium and isobutyrate are interpreted as being the consequence of pH shifts at the inner face of the plasmalemma, caused by the permeation of the undissociated form of the weak acid or base. A critical discussion of an alternative interpretation for the ammonium effect is presented.
authors.affiliation: Botanisches Institut der Universität für Bodenkultur, Gregor Mendel Straße 33, A-1180, Vienna, Austria.
authors.forename: H
authors.initials: H
authors.lastname: Löppert