title: Neutrophils are a source of gamma interferon during acute Salmonella enterica serovar Typhimurium colitis.
abstract: Gamma interferon (IFN-γ) is an important driver of intestinal inflammation during colitis caused by Salmonella enterica serovar Typhimurium. Here we used the mouse colitis model to investigate the cellular sources of IFN-γ in the cecal mucosa during the acute phase of an S. Typhimurium infection. While IFN-γ staining was detected in T cells, NK cells, and inflammatory monocytes at 2 days after infection, the majority of IFN-γ-positive cells in the cecal mucosa were neutrophils. Furthermore, neutrophil depletion blunted mucosal Ifng expression and reduced the severity of intestinal lesions during S. Typhimurium infection. We conclude that neutrophils are a prominent cellular source of IFN-γ during the innate phase of S. Typhimurium-induced colitis.
authors.affiliation: Department of Medical Microbiology and Immunology, School of Medicine, University of California at Davis, Davis, California, USA.
authors.forename: Alanna M
authors.initials: AM
authors.lastname: Spees
authors.forename: Dawn D
authors.initials: DD
authors.lastname: Kingsbury
authors.forename: Tamding
authors.initials: T
authors.lastname: Wangdi
authors.forename: Mariana N
authors.initials: MN
authors.lastname: Xavier
authors.forename: Renée M
authors.initials: RM
authors.lastname: Tsolis
authors.forename: Andreas J
authors.initials: AJ
authors.lastname: Bäumler
mesh_terms: D000208:Acute Disease; D000818:Animals; D002432:Cecum; D003092:Colitis / Q000382:microbiology*; D004195:Disease Models, Animal; D005260:Female; D007371:Interferon-gamma / Q000378:metabolism*; D007413:Intestinal Mucosa; D007694:Killer Cells, Natural / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009000:Monocytes / Q000378:metabolism; D009504:Neutrophils / Q000276:immunology*; D012480:Salmonella Infections / Q000276:immunology* / Q000473:pathology; D012485:Salmonella typhi / Q000276:immunology*; D013601:T-Lymphocytes / Q000378:metabolism
chemical_list: D007371:Interferon-gamma