title: Comparison of bacterial results from conventional cultures of the periprosthetic membrane and the synovial or pseudocapsule during hip revision arthroplasty.
abstract: BACKGROUND
  Bacterial identification is essential to diagnose and treat a revision for prosthetic loosening of an infected hip. The purpose of this study was to determine whether conventional cultures from the periprosthetic membrane are superior to synovial/pseudocapsule samples in the diagnosis of infection in hip revision arthroplasty.
  
  
  METHODS
  We performed a prospective study including all hip revisions from October 2009 to October of 2011. Once the implants were removed and prior to the administration of the antibiotic prophylaxis, six periprosthetic samples from different sites were sent to the laboratory for culturing: two periprosthetic fluid samples, two solid material (synovial/pseudocapsule) samples and two swabs. Once the six samples were taken, antibiotic prophylaxis was administered and just as the implant was removed, two more solid samples of the periprosthetic membrane were obtained.
  
  
  RESULTS
  Of a total of 86 hip revision surgeries, 22 were considered septic revisions postoperatively, of which 16 resulted in positive cultures. Of these 16 revisions, 14 obtained the same microbiologic diagnostic when considering either the synovial/pseudocapsule culture results or the solid membrane sample.
  
  
  CONCLUSIONS
  We conclude that the membrane sample for a conventional culture is not superior to the synovial/pseudocapsule sample in detecting microorganisms.
authors.affiliation: Bone and Joint Infectious Diseases Unit, Department of Orthopaedics and Trauma Surgery, Hospital Clinic of Barcelona, University of Barcelona, C/Villarroel 170, 08036, Barcelona, Spain, e.munoz.mahamud@gmail.com.
authors.forename: E
authors.initials: E
authors.lastname: Muñoz-Mahamud
authors.forename: A
authors.initials: A
authors.lastname: Soriano
authors.forename: A
authors.initials: A
authors.lastname: Combalia
authors.forename: C
authors.initials: C
authors.lastname: Medrano
authors.forename: J
authors.initials: J
authors.lastname: Bosch
authors.forename: S
authors.initials: S
authors.lastname: García
authors.forename: G
authors.initials: G
authors.lastname: Bori
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D019072:Antibiotic Prophylaxis; D019644:Arthroplasty, Replacement, Hip / Q000009:adverse effects* / Q000379:methods*; D001419:Bacteria / Q000302:isolation & purification; D005260:Female; D006622:Hip Prosthesis / Q000382:microbiology*; D006801:Humans; D008297:Male; D011446:Prospective Studies; D011475:Prosthesis Failure; D016459:Prosthesis-Related Infections / Q000188:drug therapy / Q000382:microbiology* / Q000517:prevention & control / Q000601:surgery; D012086:Reoperation / Q000379:methods; D013582:Synovial Fluid / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents