title: Oocyte-secreted factors in oocyte maturation media enhance subsequent development of bovine cloned embryos.
abstract: Successful in vitro maturation (IVM) and oocyte quality both affect the subsequent development of cloned embryos derived from somatic-cell nuclear transfer (SCNT). Developmental competence is usually lower in oocytes matured in vitro compared with those that matured in vivo, possibly due to insufficient levels of oocyte-secreted factors (OSFs) and disrupted oocyte-cumulus communication. This study investigated the effects of OSFs secreted by denuded oocytes (DOs) during IVM on the subsequent developmental competence of cloned bovine embryos. Cumulus-oocyte complexes (COCs) from antral follicles of slaughtered-cow ovaries collected from an abattoir were divided into four groups: COCs co-cultured with and without DOs in maturation media used for SCNT, as well as COCs co-cultured with and without DOs in maturation media used for in vitro fertilization (IVF). Based on the developmental competence and embryo quality of bovine embryos generated from these four groups, we found that co-culturing the COCs with DOs enhanced the in vitro development of IVF and cloned bovine embryos, and potentially generated more high-quality cloned blastocysts that possessed locus-specific histone modifications at levels similar to in vitro-fertilized embryos. These results strongly suggest that co-culturing COCs with DOs enhances subsequent developmental competence of cloned bovine embryo.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, PR China; Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, PR China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Su
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Luo
authors.forename: Zekun
authors.initials: Z
authors.lastname: Guo
authors.forename: Fusheng
authors.initials: F
authors.lastname: Quan
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000107:Acetylation; D000818:Animals; D017209:Apoptosis; D001755:Blastocyst / Q000166:cytology; D002417:Cattle; D019976:Cloning, Organism / Q000379:methods*; D018920:Coculture Techniques; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D054885:Cumulus Cells / Q000378:metabolism*; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro; D018507:Gene Expression Regulation, Developmental; D006657:Histones / Q000378:metabolism; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000378:metabolism*; D009866:Oogenesis / Q000502:physiology*; D061251:Primary Cell Culture; D011499:Protein Processing, Post-Translational
chemical_list: D017077:Culture Media, Conditioned; D006657:Histones