title: Detection of coliforms in drinking water using skin patches: a rapid, reliable method that does not require an external energy source.
abstract: The detection of coliforms requires incubation in a laboratory, generally powered using electricity. In many parts of the developing world, however, external energy sources such as electricity are not readily available. To develop a fast, reliable method for detecting coliforms in water without an external energy source, we assessed the efficacy of six test kits for the identification of coliforms in water samples. To assess the possibility of using body temperature as the sole source of heat for incubation, bacterial samples were then mixed with the enzymatic test kit reagent and attached to the human body surface using a patch system. The patches were attached to the bodies of volunteers for 24 hours and the practicality and accuracy of the patches were assessed. Coliforms were detected within 24 hours in all patches. This innovation will facilitate the testing of water quality by researchers and by economically disadvantaged people without electricity.
authors.affiliation: Water Analysis and Research Center, K-water, Daejeon, Republic of Korea; Korean Minjok Leadership Academy, Hoengseong, Gangwon, Republic of Korea.
authors.forename: Sehee
authors.initials: S
authors.lastname: Nam
authors.forename: Min-jeong
authors.initials: MJ
authors.lastname: Kim
authors.forename: MinSun
authors.initials: M
authors.lastname: Park
authors.forename: Nuri
authors.initials: N
authors.lastname: Kim
authors.forename: Yu-jin
authors.initials: YJ
authors.lastname: Lee
authors.forename: Gyu-Cheol
authors.initials: GC
authors.lastname: Lee
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D001458:Bandages; D001831:Body Temperature; D015169:Colony Count, Microbial; D060766:Drinking Water / Q000382:microbiology*; D004755:Enterobacteriaceae / Q000254:growth & development* / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D005240:Feasibility Studies; D006801:Humans; D007185:Incubators; D014871:Water Microbiology; D060753:Water Quality; D014881:Water Supply / Q000032:analysis
chemical_list: D060766:Drinking Water