title: Biosynthesis of colloidal gold nanoparticles by Streptomyces sp. NK52 and its anti-lipid peroxidation activity.
abstract: Gold nanoparticles (Au-NPs) were synthesized from chloroauric acid using cell free supernatant of Streptomyces sp. NK52 grown in nutrient broth. These nanoparticles were synthesized by varying pH and temperature of the reaction mixture and chloroauric acid concentration. The nanoparticles were characterized by spectrometry, X-ray diffraction, Scanning electron microscopy and energy dispersive spectrometry. Au-NP ranged from 10-100 nm in size and exhibited a polydispersive nature with various shapes like rods, hexagons, triangles, spheres. The diffraction peaks at 2theta = 38.1 degrees and 44.5 degrees could be assigned to the (1 1 1) and (2 0 0) planes of a faced centre cubic (fcc) lattice of gold. Au-NP showed 47% inhibition of lipid peroxidation in vitro. To the best of our knowledge, this is the first report on the rapid biosynthesis of Au-NP using cell free supernatant of Streptomyces sp. and its evaluation for anti-lipid peroxidation.
authors.affiliation: Department of Microbiology, University of Pune, Pune 411007, India.
authors.forename: Divya
authors.initials: D
authors.lastname: Prakash
authors.affiliation: Institute of Bioinformatics and Biotechnology, University of Pune, Pune 411007, India.
authors.forename: Vishal
authors.initials: V
authors.lastname: Mahale
authors.affiliation: Institute of Bioinformatics and Biotechnology, University of Pune, Pune 411007, India.
authors.forename: Ashok
authors.initials: A
authors.lastname: Bankar
authors.affiliation: Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, Dr. D. Y. Patil Vidyapeeth, Pune 411 033, India.
authors.forename: Neelu
authors.initials: N
authors.lastname: Nawani
authors.affiliation: Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, Dr. D. Y. Patil Vidyapeeth, Pune 411 033, India.
authors.forename: Vishal
authors.initials: V
authors.lastname: Mahale
authors.affiliation: Department of Microbiology, University of Pune, Pune 411007, India.
authors.forename: Divya
authors.initials: D
authors.lastname: Prakash
mesh_terms: D006046:Gold / Q000737:chemistry*; D015227:Lipid Peroxidation / Q000187:drug effects*; D053768:Metal Nanoparticles; D008855:Microscopy, Electron, Scanning; D013052:Spectrometry, X-Ray Emission; D013302:Streptomyces / Q000378:metabolism*; D014961:X-Ray Diffraction
chemical_list: D006046:Gold