title: Multiplex minisequencing screening for PTC genotype associated with bitter taste perception.
abstract: Sensitivity to phenylthiocarbamide (PTC) has a bimodal distribution pattern and the genotype of the TAS2R38 gene, which is composed of combinations of three coding single nucleotide polymorphisms (SNPs), p.A49P (c.145G>C), p.V262A (c.785T>C) and p.I296 V (c.886A>G), determines the ability or inability to taste PTC. In this study, we developed a tool for genotyping of these SNPs in the TAS2R38 gene using SNaPshot minisequencing and investigated the accuracy of the tool in 100 subjects who were genotyped by Sanger sequencing. The minor allele frequencies of the three SNPs were 0.39, and these genotypes corresponded to those determined by direct sequencing. In conclusion, we successfully developed a precise and rapid genetic tool for analysis of PTC genotype associated with bitter taste perception.
authors.affiliation: Department of Biology, College of Natural Sciences, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Borum
authors.initials: B
authors.lastname: Sagong
authors.forename: Jae Woong
authors.initials: JW
authors.lastname: Bae
authors.forename: Mee Ra
authors.initials: MR
authors.lastname: Rhyu
authors.forename: Un-Kyung
authors.initials: UK
authors.lastname: Kim
authors.forename: Mi-Kyung
authors.initials: MK
authors.lastname: Ye
mesh_terms: D005838:Genotype; D006801:Humans; D010670:Phenylthiourea / Q000302:isolation & purification*; D020641:Polymorphism, Single Nucleotide; D043562:Receptors, G-Protein-Coupled / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013649:Taste / Q000235:genetics*; D055697:Taste Perception / Q000235:genetics*; D000098706:Taste Receptors, Type 2
chemical_list: D043562:Receptors, G-Protein-Coupled; D000098706:Taste Receptors, Type 2; D010670:Phenylthiourea