title: Apical abscission alters cell polarity and dismantles the primary cilium during neurogenesis.
abstract: Withdrawal of differentiating cells from proliferative tissue is critical for embryonic development and adult tissue homeostasis; however, the mechanisms that control this cell behavior are poorly understood. Using high-resolution live-cell imaging in chick neural tube, we uncover a form of cell subdivision that abscises apical cell membrane and mediates neuron detachment from the ventricle. This mechanism operates in chick and mouse, is dependent on actin-myosin contraction, and results in loss of apical cell polarity. Apical abscission also dismantles the primary cilium, known to transduce sonic-hedgehog signals, and is required for expression of cell-cycle-exit gene p27/Kip1. We further show that N-cadherin levels, regulated by neuronal-differentiation factor Neurog2, determine cilium disassembly and final abscission. This cell-biological mechanism may mediate such cell transitions in other epithelia in normal and cancerous conditions.
authors.affiliation: Neural Development Group, Division of Cell and Developmental Biology, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK.
authors.forename: Raman M
authors.initials: RM
authors.lastname: Das
authors.forename: Kate G
authors.initials: KG
authors.lastname: Storey
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D015820:Cadherins / Q000378:metabolism; D063646:Carcinogenesis / Q000473:pathology; D002455:Cell Division; D016764:Cell Polarity; D002642:Chick Embryo; D002923:Cilia / Q000648:ultrastructure; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000378:metabolism; D051379:Mice; D009218:Myosins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D054259:Neural Tube / Q000254:growth & development / Q000378:metabolism; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D000199:Actins; D051792:Basic Helix-Loop-Helix Proteins; D015820:Cadherins; D050760:Cyclin-Dependent Kinase Inhibitor p27; D009218:Myosins; D009419:Nerve Tissue Proteins; C102938:Neurog2 protein, mouse