title: Characterization of a covalently linked complex involving ferredoxin and ferredoxin: NADP reductase.
abstract: Ferredoxin and the flavoprotein, ferredoxin: NADP reductase, have been covalently linked by incubation in the presence of a water soluble carbodiimide. The cross-linking reaction yields an adduct having a 1:1 stoichiometry. The adduct has depressed levels of diaphorase and NADPH oxidase activity and is inactive in reduction of cytochrome c using NADPH as an electron donor. Thus, although similar to an adduct described by Zanetti and coworkers [J Biol Chem 259: 6153-6157 (1984)] in its stoichiometry, the adduct described herein has significantly different enzymatic properties. It is suggested that this may be a reflection of differences in the interaction between the two proteins resulting from differences in experimental conditions in which the two adducts were prepared.
authors.affiliation: Department of Chemistry and Biochemistry, University of Arkansas, 72701, Fayetteville, AR, USA.
authors.forename: K K
authors.initials: KK
authors.lastname: Colvert
authors.forename: D J
authors.initials: DJ
authors.lastname: Davis