title: Chemical Constituents and Antimicrobial Activity of Indian Green Leafy Vegetable Cardiospermum halicacabum.
abstract: The present study was carried out to analyze chemical constituents and antibacterial activity of ethanolic leaf extract of Cardiospermum halicacabum (ECH). The FT-IR spectrum confirmed the presence of alcohols, phenols, alkanes, alkynes, aliphatic ester and flavonoids in ECH. The GC-MS analysis revealed that ECH contained about twenty four compounds. The major chemical compounds identified were cyclohexane-1, 4, 5-triol-3-one-1-carboxylic acid, benzene acetic acid, caryophyllene, phytol and neophytadiene. The ECH was screened for its antibacterial activity against different bacterial strains and anti fungal activity against Candida albicans by agar well diffusion and minimum inhibitory concentration (MIC) assay. ECH exhibited antibacterial and antifungal activity. All the tested bacterial strains showed MIC values ranging from 80 to 125 μg of extract/ml and C. albicans showed 190 μg of extract/ml as a MIC. The maximum activity ECH was observed against human pathogen Staphylococcus aureus followed by Escherichia coli and the fish pathogen Aeromonas hydrophila. ECH exhibited moderate activity against some of the tested multidrug resistant strains.
authors.affiliation: Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli, 620024 Tamil Nadu India.
authors.forename: R
authors.initials: R
authors.lastname: Jeyadevi
authors.affiliation: Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli, 620024 Tamil Nadu India.
authors.forename: T
authors.initials: T
authors.lastname: Sivasudha
authors.affiliation: Department of Microbiology, Bharathidasan University, Tiruchirappalli, 620024 Tamil Nadu India.
authors.forename: A
authors.initials: A
authors.lastname: Ilavarasi
authors.affiliation: Department of Microbiology, Bharathidasan University, Tiruchirappalli, 620024 Tamil Nadu India.
authors.forename: N
authors.initials: N
authors.lastname: Thajuddin
keywords: Antimicrobial activity; Cardiospermum halicacabum; FT-IR; GC–MS; Multidrug resistant strains