title: Isolation and purification of plastocyanin from spinach stored frozen using hydrophobic interaction and ion-exchange chromatography.
abstract: A new simple three-day procedure for preparative isolation and purification of plastocyanin from spinach stored in the frozen state is described. This procedure is based on batch adsorption on ion-exchange resin, ammonium sulphate precipitation, and purification on a Phenyl-Sepharose hydrophobic interaction column and a single Q Sepharose High Performance ion-exchange column. Approximately 100 mg of plastocyanin with an absorbance ratio A278/A597 of 1.10±0.02 in the oxidized state was typically obtained from 12 kg of spinach leaves. The purified spinach plastocyanin is shown to be homogeneous to the resolution of free solution capillary electrophoresis.
authors.affiliation: Chemistry Department A, The Technical University of Denmark, Building 207, DK-2800, Lyngby, Denmark.
authors.forename: H E
authors.initials: HE
authors.lastname: Christensen
authors.forename: L S
authors.initials: LS
authors.lastname: Conrad
authors.forename: J
authors.initials: J
authors.lastname: Ulstrup