title: The immunosuppression mechanism of hypodermin A on complement.
abstract: Hypodermin A (HA), a serine protease secreted by first-instar larvae of Hypoderma lineatum (Diptera: Oestridae) is associated with inflammatory and the specific immune responses in cattle hosts. In the present study, the cDNA sequence of HA was synthesized, and found to have fifteen amino acids which differed from the sequence available in GenBank. We then examined the association between recombinant HA and guinea-pig complement component 3 (C3) through a co-immunoprecipitation assay. Cos7 cells stably expressing HA were generated, and were found to be more resistant to lysis by guinea-pig C3 than the controls. HA was also able to degrade the C6 and C5b-9 of guinea-pig C3. The presumed DNA binding site of HA with guinea-pig C3 was detected by an electrophoretic mobility shift assay (EMSA). In contrast, after stable transfection, mHA was unable to reduce the amount of C3 or to inhibit its cytotoxicity, while HA could degrade guinea-pig C3 and inhibit the complement pathway. The findings suggest that recombinant HA could serve as an immunosuppressive agent against organ rejection after xenotransplantation.
authors.affiliation: Laboratory Animal Center, Xuzhou Medical College, Xuzhou, Jiangsu, China.
authors.forename: Ren-Jin
authors.initials: RJ
authors.lastname: Chen
authors.affiliation: Laboratory Animal Center, Xuzhou Medical College, Xuzhou, Jiangsu, China; College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China.
authors.forename: An-Kang
authors.initials: AK
authors.lastname: Hu
authors.affiliation: Department of Neurobiology, Xuzhou Medical College, Xuzhou, Jiangsu, China.
authors.forename: Hong-Hua
authors.initials: HH
authors.lastname: Yuan
authors.affiliation: Department of Cell Biology, Xuzhou Medical College, Xuzhou, Jiangsu, China.
authors.forename: Zhen-Zhen
authors.initials: ZZ
authors.lastname: Wang
authors.affiliation: Animal Science and Technology College, Yangzhou University, Yangzhou, Jiangsu, China.
authors.forename: De-Jun
authors.initials: DJ
authors.lastname: Ji
authors.affiliation: Department of Neurobiology, Xuzhou Medical College, Xuzhou, Jiangsu, China.
authors.forename: Lian-Lian
authors.initials: LL
authors.lastname: Wu
authors.affiliation: Department of Cell Biology, Xuzhou Medical College, Xuzhou, Jiangsu, China.
authors.forename: Teng-Ye
authors.initials: TY
authors.lastname: Zhang
authors.affiliation: Laboratory Animal Center, Xuzhou Medical College, Xuzhou, Jiangsu, China.
authors.forename: Yu-Hua
authors.initials: YH
authors.lastname: Zhu
authors.affiliation: Department of Immunology, Xuzhou Medical College, Xuzhou, Jiangsu, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.affiliation: Laboratory Animal Center, Xuzhou Medical College, Xuzhou, Jiangsu, China. Electronic address: zxr1967@126.com.
authors.forename: Xiao-Rong
authors.initials: XR
authors.lastname: Zhu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D003165:Complement System Proteins / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D008969:Molecular Sequence Data; D012697:Serine Endopeptidases / Q000494:pharmacology*
chemical_list: D018076:DNA, Complementary; D003165:Complement System Proteins; D012697:Serine Endopeptidases; C030092:hypodermin
keywords: Complement C3; Hypodermin A; Serine protease; Xenogeneic rejection