title: Changes in quaternary structure of muscle fructose-1,6-bisphosphatase regulate affinity of the enzyme to mitochondria.
abstract: Muscle fructose-1,6-bisphosphatase (FBP2), a regulatory enzyme of glyconeogenesis, binds to mitochondria where it interacts with proteins involved in regulation of energy homeostasis. Here, we show that the quaternary structure of FBP2 plays a crucial role in this interaction, and that the AMP-driven transition of the FBP2 subunit arrangement from active to inactive precludes its association with the mitochondria. Moreover, we demonstrate that truncation of the evolutionarily conserved N-terminal residues of FBP2 results in a loss of its mitochondria-protective functions. This strengthens the recently raised hypothesis that FBP2 evolved as a regulator not only for glycogen storage but also for mitochondrial function in contracting muscle.
authors.affiliation: Department of Animal Molecular Physiology, Wroclaw University, ul. Cybulskiego 30, 50-205 Wroclaw, Poland.
authors.forename: Michal
authors.initials: M
authors.lastname: Pirog
authors.affiliation: Department of Animal Molecular Physiology, Wroclaw University, ul. Cybulskiego 30, 50-205 Wroclaw, Poland. Electronic address: agizak@biol.uni.wroc.pl.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Gizak
authors.affiliation: Department of Animal Molecular Physiology, Wroclaw University, ul. Cybulskiego 30, 50-205 Wroclaw, Poland.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Rakus
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002460:Cell Line; D006597:Fructose-Bisphosphatase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008929:Mitochondria, Heart / Q000201:enzymology; D008931:Mitochondria, Muscle / Q000201:enzymology*; D009132:Muscles / Q000201:enzymology*; D032383:Myocytes, Cardiac / Q000201:enzymology; D010766:Phosphorylation; D020836:Protein Structure, Quaternary; D014162:Transfection
chemical_list: D006597:Fructose-Bisphosphatase; D002118:Calcium
keywords: AMP; Fructose-1,6-bisphosphatase; Mitochondria; Quaternary structure