title: Autophagy-related gene16L2, a potential serum biomarker of multiple sclerosis evaluated by bead-based proteomic technology.
abstract: Multiple sclerosis (MS) is an autoimmune disease characterized by neuroinflammation and demyelination that are mediated by T cells. The prolonged survival of autoreactive T cells acts as a primary event to trigger an inflammatory cascade that mediates myelin loss and clinical relapse in MS. Recently, T cell survival has been shown to be modulated by the autophagy-related gene (Atg). In the present study, we performed bead fractionation/matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry analyses using serum from 54 MS patients and 55 healthy controls. Eleven peptides were significantly different between the two groups with one being identified as a fragment of Atg16L2. Then the decreased levels of Atg16L2 peptides in MS patients were validated by immunoblotting and real-time PCR. As the Atg12-Atg5·Atg16 multimeric complex plays an essential role in autophagy, our results suggest that Atg16L2 may play an important role in autophagy of T cells and serve as a potential biomarker to predict clinical relapse of MS.
authors.affiliation: Department of Pharmacology, Xuan Wu Hospital of Capital Medical University, Beijing Geriatric Medical Research Center, Key Laboratory for Neurodegenerative Disease of Ministry of Education, PR China. Electronic address: yinll913@126.com.
authors.forename: Linlin
authors.initials: L
authors.lastname: Yin
authors.affiliation: Department of Neurology, Xuan Wu Hospital of Capital Medical University, 45 Changchun Street, Beijing, 100053, PR China.
authors.forename: Jianghong
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Neurology, Xuan Wu Hospital of Capital Medical University, 45 Changchun Street, Beijing, 100053, PR China.
authors.forename: Huiqing
authors.initials: H
authors.lastname: Dong
authors.affiliation: Department of Neurology, Xuan Wu Hospital of Capital Medical University, 45 Changchun Street, Beijing, 100053, PR China.
authors.forename: Erhe
authors.initials: E
authors.lastname: Xu
authors.affiliation: Department of Neurology, Xuan Wu Hospital of Capital Medical University, 45 Changchun Street, Beijing, 100053, PR China.
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Qiao
authors.affiliation: Department of Neurology, Xuan Wu Hospital of Capital Medical University, 45 Changchun Street, Beijing, 100053, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, Xuan Wu Hospital of Capital Medical University, Beijing Geriatric Medical Research Center, Key Laboratory for Neurodegenerative Disease of Ministry of Education, PR China.
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Xuan Wu Hospital of Capital Medical University, 45 Changchun Street, Beijing, 100053, PR China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Jia
authors.affiliation: Department of Pharmacology, Xuan Wu Hospital of Capital Medical University, Beijing Geriatric Medical Research Center, Key Laboratory for Neurodegenerative Disease of Ministry of Education, PR China. Electronic address: linli97@hotmail.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: National Institute for Food and Drug Control, Beijing 100050, P. R. China. Electronic address: gengxch@nicpbp.org.cn.
authors.forename: Xingchao
authors.initials: X
authors.lastname: Geng
mesh_terms: D000328:Adult; D000071183:Autophagy-Related Proteins; D015415:Biomarkers / Q000097:blood; D002352:Carrier Proteins / Q000097:blood / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology; D040901:Proteomics / Q000379:methods; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013601:T-Lymphocytes / Q000276:immunology; D055815:Young Adult
chemical_list: C518959:ATG16L1 protein, human; D000071183:Autophagy-Related Proteins; D015415:Biomarkers; D002352:Carrier Proteins
keywords: Atg16L2; T cells; multiple sclerosis; proteomic technology