title: Protein enrichment of grain sorghum by submerged culture of the amylolytic yeastsSchwanniomyces occidentalis andLipomyces kononenkoae.
abstract: Cultivation of aSchwanniomyces occidentalis derepressed mutant in a 10% (w/v) gelatinized grain sorghum slurry increased the crude protein content of the biomass from an initial value of 12% to 41% (dry) within 20 h, with no detectable residual starch. Co-cultivation ofCandida utilis with theS. occidentalis mutant improved the final crude protein content to 47% within 18 h, whereas a co-culture ofC. utilis with aLipomyces kononenkoae mutant resulted in a cultivation time of 50 h with a significantly lower protein content and a low final α-amylase activity. In a 15% (w/v) grain sorghum slurry aC. utilis/S. occidentalis co-culture increased the protein content to about 44% within 30 h. Yeast cultivation increased the lysine and threonine content of the final biomass considerably.
authors.affiliation: Department of Microbiology and Biochemistry, University of the Orange Free State, P.O. Box 339, 9300, Bloemfontein, South Africa.
authors.forename: C H
authors.initials: CH
authors.lastname: Horn
authors.forename: J C
authors.initials: JC
authors.lastname: du Preez
authors.forename: S G
authors.initials: SG
authors.lastname: Kilian