title: Arboretum and Puerto Almendras viruses: two novel rhabdoviruses isolated from mosquitoes in Peru.
abstract: Arboretum virus (ABTV) and Puerto Almendras virus (PTAMV) are two mosquito-associated rhabdoviruses isolated from pools of Psorophora albigenu and Ochlerotattus fulvus mosquitoes, respectively, collected in the Department of Loreto, Peru, in 2009. Initial tests suggested that both viruses were novel rhabdoviruses and this was confirmed by complete genome sequencing. Analysis of their 11 482 nt (ABTV) and 11 876 (PTAMV) genomes indicates that they encode the five canonical rhabdovirus structural proteins (N, P, M, G and L) with an additional gene (U1) encoding a small hydrophobic protein. Evolutionary analysis of the L protein indicates that ABTV and PTAMV are novel and phylogenetically distinct rhabdoviruses that cannot be classified as members of any of the eight currently recognized genera within the family Rhabdoviridae, highlighting the vast diversity of this virus family.
authors.affiliation: Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX, USA.|Center for Tropical Diseases, University of Texas Medical Branch, Galveston, TX, USA.|Center for Biodefense and Emerging Infectious Diseases and Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
authors.forename: Nikos
authors.initials: N
authors.lastname: Vasilakis
authors.affiliation: US Naval Medical Research Unit no. 6, Lima, Peru.
authors.forename: Fanny
authors.initials: F
authors.lastname: Castro-Llanos
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, USA.
authors.forename: Steven G
authors.initials: SG
authors.lastname: Widen
authors.affiliation: Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX, USA.|Center for Tropical Diseases, University of Texas Medical Branch, Galveston, TX, USA.|Center for Biodefense and Emerging Infectious Diseases and Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
authors.forename: Patricia V
authors.initials: PV
authors.lastname: Aguilar
authors.affiliation: Center for Biodefense and Emerging Infectious Diseases and Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
authors.forename: Hilda
authors.initials: H
authors.lastname: Guzman
authors.affiliation: US Naval Medical Research Unit no. 6, Lima, Peru.
authors.forename: Carolina
authors.initials: C
authors.lastname: Guevara
authors.affiliation: US Naval Medical Research Unit no. 6, Lima, Peru.
authors.forename: Roberto
authors.initials: R
authors.lastname: Fernandez
authors.affiliation: Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX, USA.|Center for Biodefense and Emerging Infectious Diseases and Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
authors.forename: Albert J
authors.initials: AJ
authors.lastname: Auguste
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, USA.
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Wood
authors.affiliation: Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX, USA.|Center for Biodefense and Emerging Infectious Diseases and Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
authors.forename: Vsevolod
authors.initials: V
authors.lastname: Popov
authors.affiliation: US Naval Medical Research Unit no. 6, Lima, Peru.
authors.forename: Kirk
authors.initials: K
authors.lastname: Mundal
authors.affiliation: Center for Vaccine Research, Department of Computational and Systems Biology, University of Pittsburgh, Pittsburgh, PA, USA.
authors.forename: Elodie
authors.initials: E
authors.lastname: Ghedin
authors.affiliation: Naval Medical Research Center, Silver Spring, MD, USA.
authors.forename: Tadeusz J
authors.initials: TJ
authors.lastname: Kochel
authors.affiliation: Marie Bashir Institute for Infectious Diseases and Biosecurity, School of Biological Sciences and Sydney Medical School, University of Sydney, Sydney, NSW 2006, Australia.
authors.forename: Edward C
authors.initials: EC
authors.lastname: Holmes
authors.affiliation: CSIRO Animal, Food and Health Sciences, Australian Animal Health Laboratory, Geelong, VIC 3220, Australia.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Walker
authors.affiliation: Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX, USA.|Center for Tropical Diseases, University of Texas Medical Branch, Galveston, TX, USA.|Center for Biodefense and Emerging Infectious Diseases and Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
authors.forename: Robert B
authors.initials: RB
authors.lastname: Tesh
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D009033:Culicidae / Q000821:virology*; D005260:Female; D016679:Genome, Viral; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010568:Peru; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D012209:Rhabdoviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics; D014771:Virion / Q000648:ultrastructure
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins