title: In vivo anticoccidial activity of berberine [18, 5,6-dihydro-9,10-dimethoxybenzo(g)-1,3-benzodioxolo(5,6-a) quinolizinium]--an isoquinoline alkaloid present in the root bark of Berberis lycium.
abstract: Coccidiosis, caused by various Eimeria species, is a major parasitic disease in chicken. However the increasing resistance of these parasites to currently used anticoccidial drugs has stimulated the search for new methods of control. As part of this effort we investigated the root bark of Berberis lycium (barberry) as a potential source of compounds with anticoccidial activity. In the present study anticoccidial activity of different solvent extracts of the root bark of B. lycium and berberine was evaluated in vivo using broiler chicken. Results of the study demonstrated equipotent efficacy of pure berberine in comparison to that of standard drug amprolium on the basis of reduction in coccidian oocyst output, body weight gain of chicken and feed conversion ratio. Among the extracts crude methanolic extract showed highest anticoccidial activity tested at 300 mg/kg body weight which could be due to the presence of alcohol-soluble active ingredients in root bark of B. lycium. Toxicological studies revealed that B. lycium extracts as well as berberine were not lethal up to dosage of 2000 mg/kg body weight. LD(50) was not determined as mortalities were not recorded in any of the five groups of chicken. From the present study it can be concluded that root bark of B. lycium has the immense potential to contribute to the control of coccidian parasites of chicken. Our results corroborate the use of berberine for treatment of severe diarrhoea, amoebiasis and intestinal infections and could justify its use in folk medicine for treatment of haemorrhagic dysentery.
authors.affiliation: Centre of Research for Development, University of Kashmir, Srinagar 190006, Jammu & Kashmir, India. Electronic address: maliktsf@gmail.com.
authors.forename: Tauseef Ahmad
authors.initials: TA
authors.lastname: Malik
authors.affiliation: Centre of Research for Development, University of Kashmir, Srinagar 190006, Jammu & Kashmir, India.
authors.forename: Azra N
authors.initials: AN
authors.lastname: Kamili
authors.affiliation: Centre of Research for Development, University of Kashmir, Srinagar 190006, Jammu & Kashmir, India.
authors.forename: M Z
authors.initials: MZ
authors.lastname: Chishti
authors.affiliation: Department of Zoology, University of Kashmir, Srinagar 190006, Jammu & Kashmir, India.
authors.forename: Syed
authors.initials: S
authors.lastname: Tanveer
authors.affiliation: Department of Zoology, University of Kashmir, Srinagar 190006, Jammu & Kashmir, India.
authors.forename: Shazia
authors.initials: S
authors.lastname: Ahad
authors.affiliation: PK-PD Toxicology Division, Indian Institute of Integrative Medicine (CSIR), Jammu-Tawi, India.
authors.forename: R K
authors.initials: RK
authors.lastname: Johri
mesh_terms: D000818:Animals; D001599:Berberine / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D028142:Berberis / Q000737:chemistry*; D002645:Chickens; D003048:Coccidiosis / Q000517:prevention & control / Q000662:veterinary*; D003049:Coccidiostats / Q000302:isolation & purification*; D008297:Male; D010270:Parasite Egg Count; D008517:Phytotherapy; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000627:therapeutic use; D018517:Plant Roots / Q000737:chemistry; D011201:Poultry Diseases / Q000517:prevention & control*; D015430:Weight Gain / Q000187:drug effects
chemical_list: D003049:Coccidiostats; D010936:Plant Extracts; D001599:Berberine
keywords: Anticoccidial activity; Berberine; Berberis lycium; Poultry