title: Influence of elevated levels of C-reactive protein on circulating endothelial progenitor cell function.
abstract: In vitro, C-reactive protein (CRP) impairs endothelial progenitor cell (EPC) function; however, the influence of CRP on EPCs in vivo is unclear. We determined whether EPC function is impaired in adults with elevated plasma CRP concentrations, independent of other risk factors. EPCs were harvested from 75 adults (43 males, 32 females): 25 with low CRP (<1.0 mg/L); 25 with moderate CRP (1.0-3.0 mg/L); and 25 with high CRP (>3.0 mg/L). The capacity of EPCs to form colonies (colony assay), migrate (Boyden chamber), release angiogenic growth factor (ELISA) and resist apoptosis (active caspase-3) was determined. There were no significant differences between the CRP groups in EPC colony formation (CFU), migration (AU) or the ability to release vascular endothelial growth factor (VEGF; pg/mL): low (13 ± 3 CFU; 1255 ± 100 AU; 126 ± 24 pg/mL); moderate (11 ± 3 CFU; 1137 ± 85 AU; 97 ± 14 pg/mL); and high (13 ± 4 CFU; 1071 ± 80 AU; 119 ± 22 pg/mL) CRP. Staurosporine-stimulated activation of caspase-3 was also similar between the low (2.3 ± 0.2 ng/mL), moderate (2.1 ± 0.3 ng/mL), and high (2.2 ± 0.2 ng/mL) CRP groups. These results indicate that elevations in plasma CRP are not associated with impaired EPC function. EPC dysfunction may not play a role in CRP-related cardiovascular risk.
authors.affiliation: Integrative Vascular Biology Laboratory, Department of Integrative Physiology, University of Colorado, Boulder, Colorado, USA.
authors.forename: Kevin A
authors.initials: KA
authors.lastname: Fasing
authors.forename: Benjamin J
authors.initials: BJ
authors.lastname: Nissan
authors.forename: Jared J
authors.initials: JJ
authors.lastname: Greiner
authors.forename: Brian L
authors.initials: BL
authors.lastname: Stauffer
authors.forename: Christopher A
authors.initials: CA
authors.lastname: DeSouza
mesh_terms: D002097:C-Reactive Protein / Q000378:metabolism*; D002465:Cell Movement; D003114:Colony-Forming Units Assay; D066026:Endothelial Progenitor Cells / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D042461:Vascular Endothelial Growth Factor A; D002097:C-Reactive Protein
keywords: CRP; apoptosis; progenitor cells