title: Crystallization and structure determination of a symmetrical 'football' complex of the mammalian mitochondrial Hsp60-Hsp10 chaperonins.
abstract: The mitochondrial Hsp60-Hsp10 complex assists the folding of various proteins impelled by ATP hydrolysis, similar to the bacterial chaperonins GroEL and GroES. The near-atomic structural details of the mitochondrial chaperonins are not known, despite the fact that almost two decades have passed since the structures of the bacterial chaperonins became available. Here, the crystallization procedure, diffraction experiments and structure determination by molecular replacement of the mammalian mitochondrial chaperonin HSP60 (E321K mutant) and its co-chaperonin Hsp10 are reported.
authors.affiliation: Department of Biochemistry and Molecular Biology, George S. Wise Faculty of Life Sciences, Tel Aviv University, 69978 Tel Aviv, Israel.
authors.forename: Shahar
authors.initials: S
authors.lastname: Nisemblat
authors.affiliation: Department of Biochemistry and Molecular Biology, George S. Wise Faculty of Life Sciences, Tel Aviv University, 69978 Tel Aviv, Israel.
authors.forename: Avital
authors.initials: A
authors.lastname: Parnas
authors.affiliation: The Daniella Rich Institute for Structural Biology, George S. Wise Faculty of Life Sciences, Tel Aviv University, 69978 Tel Aviv, Israel.
authors.forename: Oren
authors.initials: O
authors.lastname: Yaniv
authors.affiliation: Department of Biochemistry and Molecular Biology, George S. Wise Faculty of Life Sciences, Tel Aviv University, 69978 Tel Aviv, Israel.
authors.forename: Abdussalam
authors.initials: A
authors.lastname: Azem
authors.affiliation: The Daniella Rich Institute for Structural Biology, George S. Wise Faculty of Life Sciences, Tel Aviv University, 69978 Tel Aviv, Israel.
authors.forename: Felix
authors.initials: F
authors.lastname: Frolow
mesh_terms: D000818:Animals; D018835:Chaperonin 10 / Q000737:chemistry*; D018834:Chaperonin 60 / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D008322:Mammals / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*
chemical_list: D018835:Chaperonin 10; D018834:Chaperonin 60
keywords: mHsp10; mHsp60; mitochondrial chaperonins