title: A novel DRAK inhibitor, SC82510, promotes axon branching of adult sensory neurons in vitro.
abstract: Recently, a new potent protein kinase inhibitor, SC82510, was identified acting on DRAK2 and stimulating axon outgrowth at low concentrations. DRAK is the Drosophila homologue of death-associated protein kinase that phosphorylates myosin-II regulatory light chain in a similar fashion as ROCK, the downstream target of RhoA mediating axon outgrowth inhibition. While higher concentrations of this novel compound exhibited toxic effects, significant promotion of process outgrowth of PC12 cells and of adult primary neurons was observed at 1 nM which could be further enhanced by addition of a neuronal growth factor (FGF-2). Unlike the effects of ROCK inhibitors on axon outgrowth that stimulate both, elongation and branching, SC82510 primarily promoted axon branching, whereas axon elongation was not increased in this cell culture model of peripheral axon regeneration.
authors.affiliation: Division of Neuroanatomy, Department of Anatomy, Histology and Embryology, Medical University of Innsbruck, Muellerstrasse 59, 6020, Innsbruck, Austria.
authors.forename: Letizia
authors.initials: L
authors.lastname: Marvaldi
authors.forename: Barbara
authors.initials: B
authors.lastname: Hausott
authors.forename: Maria
authors.initials: M
authors.lastname: Auer
authors.forename: Johann
authors.initials: J
authors.lastname: Leban
authors.forename: Lars
authors.initials: L
authors.lastname: Klimaschewski
mesh_terms: D000818:Animals; D003470:Culture Media; D066298:In Vitro Techniques; D016716:PC12 Cells; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D051381:Rats; D011984:Sensory Receptor Cells / Q000187:drug effects*
chemical_list: D003470:Culture Media; D047428:Protein Kinase Inhibitors