title: Rapid and simple isolation of pure photosystem II core and reaction center particles from spinach.
abstract: Pure and active oxygen-evolving PS II core particles containing 35 Chl per reaction center were isolated with 75% yield from spinach PS II membrane fragments by incubation with n-dodecyl-β-D-maltoside and a rapid one step anion-exchange separation. By Triton X-100 treatment on the column these particles could be converted with 55% yield to pure and active PS II reaction center particles, which contained 6 Chl per reaction center.
authors.affiliation: Department of Biophysics, Huygens laboratory of the State University, P.O. Box 9504, 2300 RA, Leiden.
authors.forename: P J
authors.initials: PJ
authors.lastname: van Leeuwen
authors.forename: M C
authors.initials: MC
authors.lastname: Nieveen
authors.forename: E J
authors.initials: EJ
authors.lastname: van de Meent
authors.forename: J P
authors.initials: JP
authors.lastname: Dekker
authors.forename: H J
authors.initials: HJ
authors.lastname: van Gorkom