title: Development of Loop-Mediated Isothermal Amplification (LAMP) for Universal Detection of Enteroviruses.
abstract: Enteroviruses are found in most environments and cause several diseases in humans. Loop-mediated isothermal amplification (LAMP) was adapted and evaluated for the rapid detection of enteroviruses. Based on the highly conserved 5' untranslated region (5'-UTR) of the human enteroviruses (HEVs), particularly human enterovirus A (HEV-A) and HEV-B, a set of universal primers was designed. The LAMP amplification was carried out under isothermal conditions at 61 °C, depending on the template concentration results were obtained within 45-90 min. The detection limits were found to be 10(1) copies of cloned enterovirus 71 fragments, more sensitive than conventional PCR. Nine water samples collected from drinking water sources during three seasons and 19 stool specimens collected from HFMD patients were analyzed. By using the LAMP assay, the majority of samples was tested positive, 9/9 (100 %) and 18/19 (94.7 %), respectively. LAMP is a practical method for the rapid detection of enteroviruses in environmental and clinical samples.
authors.affiliation: Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Logistics University of Chinese People's Armed Police Forces, Tianjin, 300162 China.
authors.forename: Hua-Bing
authors.initials: HB
authors.lastname: Zhao
authors.affiliation: Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Logistics University of Chinese People's Armed Police Forces, Tianjin, 300162 China.
authors.forename: Guang-Ya
authors.initials: GY
authors.lastname: Yin
authors.affiliation: Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Logistics University of Chinese People's Armed Police Forces, Tianjin, 300162 China.
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Zhao
authors.affiliation: Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Logistics University of Chinese People's Armed Police Forces, Tianjin, 300162 China.
authors.forename: Ai-Hua
authors.initials: AH
authors.lastname: Huang
authors.affiliation: Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Logistics University of Chinese People's Armed Police Forces, Tianjin, 300162 China.
authors.forename: Jun-Hong
authors.initials: JH
authors.lastname: Wang
authors.affiliation: Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Logistics University of Chinese People's Armed Police Forces, Tianjin, 300162 China.
authors.forename: Shuang-Feng
authors.initials: SF
authors.lastname: Yang
authors.affiliation: Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Logistics University of Chinese People's Armed Police Forces, Tianjin, 300162 China.
authors.forename: Hong-Sheng
authors.initials: HS
authors.lastname: Gao
authors.affiliation: School of Public Health, Hebei Medical University, Shijiazhuang, 050031 China.
authors.forename: Wei-Jun
authors.initials: WJ
authors.lastname: Kang
keywords: Detection; Drinking water source; Enterovirus; HFMD; LAMP