title: Conformational heterogeneity in antibody-protein antigen recognition: implications for high affinity protein complex formation.
abstract: Specific, high affinity protein-protein interactions lie at the heart of many essential biological processes, including the recognition of an apparently limitless range of foreign proteins by natural antibodies, which has been exploited to develop therapeutic antibodies. To mediate biological processes, high affinity protein complexes need to form on appropriate, relatively rapid timescales, which presents a challenge for the productive engagement of complexes with large and complex contact surfaces (∼600-1800 Å(2)). We have obtained comprehensive backbone NMR assignments for two distinct, high affinity antibody fragments (single chain variable and antigen-binding (Fab) fragments), which recognize the structurally diverse cytokines interleukin-1β (IL-1β, β-sheet) and interleukin-6 (IL-6, α-helical). NMR studies have revealed that the hearts of the antigen binding sites in both free anti-IL-1β Fab and anti-IL-6 single chain variable exist in multiple conformations, which interconvert on a timescale comparable with the rates of antibody-antigen complex formation. In addition, we have identified a conserved antigen binding-induced change in the orientation of the two variable domains. The observed conformational heterogeneity and slow dynamics at protein antigen binding sites appears to be a conserved feature of many high affinity protein-protein interfaces structurally characterized by NMR, suggesting an essential role in protein complex formation. We propose that this behavior may reflect a soft capture, protein-protein docking mechanism, facilitating formation of high affinity protein complexes on a timescale consistent with biological processes.
authors.affiliation: Department of Biochemistry, University of Leicester, Leicester LE1 9HN, United Kingdom.
authors.forename: Philip W
authors.initials: PW
authors.lastname: Addis
authors.affiliation: Department of Biochemistry, University of Leicester, Leicester LE1 9HN, United Kingdom.
authors.forename: Catherine J
authors.initials: CJ
authors.lastname: Hall
authors.affiliation: Department of Biochemistry, University of Leicester, Leicester LE1 9HN, United Kingdom.
authors.forename: Shaun
authors.initials: S
authors.lastname: Bruton
authors.affiliation: Department of Biochemistry, University of Leicester, Leicester LE1 9HN, United Kingdom.
authors.forename: Vaclav
authors.initials: V
authors.lastname: Veverka
authors.affiliation: Department of Biochemistry, University of Leicester, Leicester LE1 9HN, United Kingdom.
authors.forename: Ian C
authors.initials: IC
authors.lastname: Wilkinson
authors.affiliation: Department of Biochemistry, University of Leicester, Leicester LE1 9HN, United Kingdom.
authors.forename: Frederick W
authors.initials: FW
authors.lastname: Muskett
authors.affiliation: Department of Biochemistry, University of Leicester, Leicester LE1 9HN, United Kingdom.
authors.forename: Philip S
authors.initials: PS
authors.lastname: Renshaw
authors.affiliation: Department of Biochemistry, University of Leicester, Leicester LE1 9HN, United Kingdom; Research and Development, UCB, Slough SL1 3WE, United Kingdom.
authors.forename: Christine E
authors.initials: CE
authors.lastname: Prosser
authors.affiliation: Research and Development, UCB, Slough SL1 3WE, United Kingdom.
authors.forename: Bruce
authors.initials: B
authors.lastname: Carrington
authors.affiliation: Research and Development, UCB, Slough SL1 3WE, United Kingdom.
authors.forename: Alastair D G
authors.initials: ADG
authors.lastname: Lawson
authors.affiliation: Research and Development, UCB, Slough SL1 3WE, United Kingdom.
authors.forename: Robert
authors.initials: R
authors.lastname: Griffin
authors.affiliation: Research and Development, UCB, Slough SL1 3WE, United Kingdom.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Taylor
authors.affiliation: Department of Biochemistry, University of Leicester, Leicester LE1 9HN, United Kingdom. Electronic address: lw83@le.ac.uk.
authors.forename: Lorna C
authors.initials: LC
authors.lastname: Waters
authors.affiliation: Research and Development, UCB, Slough SL1 3WE, United Kingdom. Electronic address: alistair.henry@ucb.com.
authors.forename: Alistair J
authors.initials: AJ
authors.lastname: Henry
authors.affiliation: Department of Biochemistry, University of Leicester, Leicester LE1 9HN, United Kingdom. Electronic address: mdc12@le.ac.uk.
authors.forename: Mark D
authors.initials: MD
authors.lastname: Carr
mesh_terms: D000595:Amino Acid Sequence; D061067:Antibodies, Monoclonal, Humanized / Q000737:chemistry*; D000915:Antibody Affinity; D000936:Antigen-Antibody Complex / Q000737:chemistry*; D000941:Antigens / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry*; D053583:Interleukin-1beta / Q000737:chemistry / Q000276:immunology*; D015850:Interleukin-6 / Q000737:chemistry / Q000276:immunology*; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D000936:Antigen-Antibody Complex; D000941:Antigens; D007140:Immunoglobulin Fab Fragments; D053583:Interleukin-1beta; D015850:Interleukin-6
keywords: Antibodies; CDR; Conformational Heterogeneity; NMR; Protein Conformation; Protein Structure; Protein-Protein Interactions