title: Electrospun nanofibrous scaffolds of poly (L-lactic acid)-dicalcium silicate composite via ultrasonic-aging technique for bone regeneration.
abstract: Polymeric nanofibrous composite scaffolds incorporating bioglass and bioceramics have been increasingly promising for bone tissue engineering. In the present study, electrospun poly (l-lactic acid) (PLLA) scaffolds containing dicalcium silicate (C2S) nanoparticles (approximately 300 nm) were fabricated. Using a novel ultrasonic dispersion and aging method, uniform C2S nanoparticles were prepared and they were homogenously distributed in the PLLA nanofibers upon electrospinning. In vitro, the PLLA-C2S fibers induced the formation of HAp on the surface when immersed in simulated body fluid (SBF). During culture, the osteoblastic MC3T3-E1 cells adhered well on PLLA-C2S scaffolds, as evidenced by the well-defined actin stress fibers and well-spreading morphology. Further, compared to pure PLLA scaffolds without C2S, PLLA-C2S scaffolds markedly promoted the proliferation of MC3T3-E1 cells as well as their osteogenic differentiation, which was characterized by the enhanced alkaline phosphatase (ALP) activity. Together, findings from this study clearly demonstrated that PLLA-C2S composite scaffold may function as an ideal candidate for bone tissue engineering.
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 188 Shizi St, Suzhou, Jiangsu 215006, China.
authors.forename: Shengjie
authors.initials: S
authors.lastname: Dong
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 188 Shizi St, Suzhou, Jiangsu 215006, China. Electronic address: wodaoshi@sohu.com.
authors.forename: Junying
authors.initials: J
authors.lastname: Sun
authors.affiliation: College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, Jiangsu 215123, China.
authors.forename: Yadong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 188 Shizi St, Suzhou, Jiangsu 215006, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Orthopedic Institute, Soochow University, 708 Renmin Rd, Suzhou, Jiangsu 215007, China.
authors.forename: Wenguo
authors.initials: W
authors.lastname: Cui
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 188 Shizi St, Suzhou, Jiangsu 215006, China. Electronic address: binli@suda.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D017610:Calcium Compounds / Q000737:chemistry* / Q000528:radiation effects; D049109:Cell Proliferation; D004563:Electrochemistry / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D048091:Guided Tissue Regeneration / Q000295:instrumentation*; D019344:Lactic Acid / Q000737:chemistry* / Q000528:radiation effects; D008422:Materials Testing; D051379:Mice; D057139:Nanofibers / Q000737:chemistry* / Q000528:radiation effects / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry* / Q000528:radiation effects; D012399:Rotation; D017640:Silicates / Q000737:chemistry* / Q000528:radiation effects; D013010:Sonication; D054457:Tissue Scaffolds
chemical_list: D017610:Calcium Compounds; D011091:Polyesters; D011108:Polymers; D017640:Silicates; C013481:dicalcium silicate; D019344:Lactic Acid; C033616:poly(lactide)
keywords: Biomineralization; Cell proliferation; Dicalcium silicate; Differentiation; Electrospinning