title: Proteomic analysis of pakchoi leaves and roots under glycine-nitrogen conditions.
abstract: The physiological and differential proteomic responses of pakchoi leaves and roots to glycine-nitrogen (Gly-N) treatments were determined. Two pakchoi (Brassica campestris ssp. chinensis L. Makino. var. communis Tsen et Lee) cultivars, 'Huawang' and 'Wuyueman', were grown under sterile hydroponic conditions with different N forms (Gly-N and nitrate-N). Gly-N-treated pakchoi exhibited decreased fresh weights, total N uptake, leaf areas, and net photosynthetic rates than those treated with nitrate-N. Differentially regulated proteins were selected after image analysis and identified using MALDI-TOF MS. A total of 23 proteins was up- or down-regulated following Gly-N treatment. These spots are involved in several processes, such as energy synthesis, N metabolism, photosynthesis, and active antioxidant defense mechanisms, that could enhance plant adaptation to Gly-N. The superior Gly tolerance of 'Huawang' was predominantly associated with a less severe down-regulation of proteins that are involved in the electron transport chain and N metabolism. Other factors could include less ribulose-1,5-bisphosphate carboxylase/oxygenase turnover or a higher up-regulation of stress defense proteins. These characteristics demonstrated that maintaining ATP synthesis, N metabolism, photosynthesis, and active defense mechanisms play a critical role in pakchoi adaptation to Gly-N.
authors.affiliation: Department of Horticulture, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, PR China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Horticulture, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, PR China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Tang
authors.affiliation: Department of Horticulture, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, PR China. Electronic address: danfenggrace@gmail.com.
authors.forename: Danfeng
authors.initials: D
authors.lastname: Huang
mesh_terms: D000222:Adaptation, Physiological; D000255:Adenosine Triphosphate / Q000378:metabolism; D001937:Brassica / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology; D005998:Glycine / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism*; D010788:Photosynthesis; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D013045:Species Specificity; D013312:Stress, Physiological
chemical_list: D010940:Plant Proteins; D020543:Proteome; D000255:Adenosine Triphosphate; D009584:Nitrogen; D005998:Glycine
keywords: 2-DE; 3-[(3-cholamido-propyl)-dimethylammonio]-1-propanesulfonate; Amino acid; CA; CAN; CBB; CCS; CHAPS; Coomassie brilliant blue; DTT; FNR; GR-RBP; GS; Gly-rich RNA-binding protein; Glycine; IPG; MALDI-TOF; MS; Nitrate; PR; Pakchoi; Pn; Proteomics; ROS; RSS; Reactive oxygen species; Rubisco; S-adenosylmethionine synthase isozyme; SAMS; TCTP; acetonitrile; carbonic anhydrase; copper chaperone; dithiothreitol; ferredoxin-NADP(+) reductase; glutamine synthetase; immobilized pH gradient; mass spectrometry; matrix-assisted laser desorption and ionization time-of-flight; net photosynthesis rate; putative pathogenesis-related protein; reactive sulfur species; ribulose-1,5-bisphosphate carboxylase/oxygenase; translationally-controlled tumor protein homolog; two-dimensional gel electrophoresis