title: Carboxylicivirga gen. nov. in the family Marinilabiliaceae with two novel species, Carboxylicivirga mesophila sp. nov. and Carboxylicivirga taeanensis sp. nov., and reclassification of Cytophaga fermentans as Saccharicrinis fermentans gen. nov., comb. nov.
abstract: Two facultatively anaerobic mesophilic bacteria, strains MEBiC 07026(T) and MEBiC 08903(T), were isolated from two different tidal flat sediments and both strains showed approximately 92.2 % 16S rRNA gene sequence similarity with [Cytophaga] fermentans DSM 9555(T). 16S rRNA gene sequence similarity between the two new isolates was 97.5 % but levels of DNA-DNA relatedness between the two were 31.3-31.8 %. Phylogenetic analysis revealed that the two isolates and [Cytophaga] fermentans DSM 9555(T) were affiliated with the family Marinilabiliaceae in the class Bacteroidia. The dominant fatty acids of strains MEBiC 07026(T), MEBiC 08903(T) and [Cytophaga] fermentans DSM 9555(T) were branched-type or hydroxylated C15 : 0, but [Cytophaga] fermentans DSM 9555(T) contained a higher proportion of anteiso-branched fatty acids. The two new isolates contained a markedly higher proportion of monounsaturated fatty acids than other members of the family Marinilabiliaceae. The major respiratory quinone of the strains was MK-7. Strains MEBiC07026(T) and MEBiC08903(T) utilized a wide range of carboxylic acids whereas [Cytophaga] fermentans DSM 9555(T) utilized carbohydrates rather than carboxylic acids. The DNA G+C content of the novel strains was about 44 mol% but that of [Cytophaga] fermentans DSM 9555(T) revealed from the genome sequence was 37.6 mol%. Based on evidence from this polyphasic taxonomic study, a novel genus, Carboxylicivirga gen. nov., is proposed in the family Marinilabiliaceae with two novel species, Carboxylicivirga mesophila sp. nov. with type strain MEBiC 07026(T) ( = KCCM 42978(T) = JCM 18290(T)) and Carboxylicivirga taeanensis sp. nov. with type strain MEBiC 08903(T) ( = KCCM 43024(T) = JCM 19490(T)). Additionally, [Cytophaga] fermentans DSM 9555(T) ( = ATCC 19072(T)) is reclassified as Saccharicrinis fermentans gen. nov., comb. nov.
authors.affiliation: Marine Biotechnology Research Division, Korea Institute of Ocean Science & Technology, PO Box 29, Ansan 425-600, Republic of Korea.
authors.forename: Sung-Hyun
authors.initials: SH
authors.lastname: Yang
authors.affiliation: Marine Biotechnology Research Division, Korea Institute of Ocean Science & Technology, PO Box 29, Ansan 425-600, Republic of Korea.
authors.forename: Hyun-Seok
authors.initials: HS
authors.lastname: Seo
authors.affiliation: Gyengbuk Institute for Marine Bio-Industry, Uljin, Gyeongbuk 767-813, Republic of Korea.
authors.forename: Jung-Hee
authors.initials: JH
authors.lastname: Woo
authors.affiliation: Marine Biotechnology Research Division, Korea Institute of Ocean Science & Technology, PO Box 29, Ansan 425-600, Republic of Korea.
authors.forename: Hyun-Myung
authors.initials: HM
authors.lastname: Oh
authors.affiliation: Marine Biotechnology Research Division, Korea Institute of Ocean Science & Technology, PO Box 29, Ansan 425-600, Republic of Korea.
authors.forename: Hani
authors.initials: H
authors.lastname: Jang
authors.affiliation: Marine Biotechnology Research Division, Korea Institute of Ocean Science & Technology, PO Box 29, Ansan 425-600, Republic of Korea.
authors.forename: Jung-Hyun
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Marine Biotechnology Research Division, Korea Institute of Ocean Science & Technology, PO Box 29, Ansan 425-600, Republic of Korea.
authors.forename: Sang-Jin
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Marine Biotechnology Research Division, Korea Institute of Ocean Science & Technology, PO Box 29, Ansan 425-600, Republic of Korea.
authors.forename: Kae Kyoung
authors.initials: KK
authors.lastname: Kwon
mesh_terms: D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D003589:Cytophaga / Q000145:classification*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C062629:menaquinone 7