title: Fabricating a cycloolefin polymer immunoassay platform with a dual-function polymer brush via a surface-initiated photoiniferter-mediated polymerization strategy.
abstract: The development of technologies for a biomedical detection platform is critical to meet the global challenges of various disease diagnoses. In this study, an inert cycloolefin polymer (COP) support was modified with two-layer polymer brushes possessing dual functions, i.e., a low fouling poly[poly(ethylene glycol) methacrylate] [p(PEGMA)] bottom layer and a poly(acrylic acid) (PAA) upper layer for antibody loading, via a surface-initiated photoiniferter-mediated polymerization strategy for fluorescence-based immunoassay. It was demonstrated through a confocal laser scanner that, for the as-prepared COP-g-PEG-b-PAA-IgG supports, nonspecific protein adsorption was suppressed, and the resistance to nonspecific protein interference on antigen recognition was significantly improved, relative to the COP-g-PAA-IgG references. This strategy for surface modification of a polymeric platform is also applicable to the fabrication of other biosensors.
authors.affiliation: State Key Laboratory of Polymer and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun 130022, People's Republic of China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Ma
authors.forename: Shifang
authors.initials: S
authors.lastname: Luan
authors.forename: Lingjie
authors.initials: L
authors.lastname: Song
authors.forename: Jing
authors.initials: J
authors.lastname: Jin
authors.forename: Shuaishuai
authors.initials: S
authors.lastname: Yuan
authors.forename: Shunjie
authors.initials: S
authors.lastname: Yan
authors.forename: Huawei
authors.initials: H
authors.lastname: Yang
authors.forename: Hengchong
authors.initials: H
authors.lastname: Shi
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yin
mesh_terms: D000327:Adsorption; D000475:Alkenes / Q000737:chemistry*; D000818:Animals; D000906:Antibodies / Q000378:metabolism; D000941:Antigens / Q000378:metabolism; D002417:Cattle; D005453:Fluorescence; D055767:Immobilized Proteins / Q000378:metabolism; D007118:Immunoassay / Q000379:methods*; D018625:Microscopy, Atomic Force; D056951:Photoelectron Spectroscopy; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: D000475:Alkenes; D000906:Antibodies; D000941:Antigens; D055767:Immobilized Proteins; D011108:Polymers; D012710:Serum Albumin, Bovine