title: Low-power polarization transfer between deuterons and spin-1/2 nuclei using adiabatic (RESPIRATION)CP in solid-state NMR.
abstract: Establishing high-resolution structures of biological macromolecules in heterogeneous environments by MAS solid-state NMR is an important challenge where development of advanced experimental procedures is in high demand. Promising new methods take advantage of samples with extensive (2)H, (13)C, and (15)N isotope labelling, effectively diluting (1)H spins. In many cases, a sufficient amount of (1)H at exchangeable sites cannot be re-established during the purification procedure, hence it is necessary to exploit also the potential of (2)H as a starting point in pulse sequences, capitalizing on its short T1 as compared to (13)C, and to detect carbon or proton spins as appropriate. Here we present a new method that enables the required high-efficiency (2)H to (13)C or (15)N polarization transfer to be accomplished under the limited (2)H rf power conditions using current (1)H, (2)H, (13)C and (15)N quadruple-resonance MAS NMR instrumentation.
authors.affiliation: Center for Insoluble Protein Structures (inSPIN), Interdiscliplinary Nanoscience Center (iNANO) and Department of Chemistry, Aarhus University, Gustav Wieds Vej 14, DK-8000 Aarhus C, Denmark. ncn@inano.au.dk.
authors.forename: Sheetal K
authors.initials: SK
authors.lastname: Jain
authors.forename: Anders B
authors.initials: AB
authors.lastname: Nielsen
authors.forename: Matthias
authors.initials: M
authors.lastname: Hiller
authors.forename: Liselotte
authors.initials: L
authors.lastname: Handel
authors.forename: Matthias
authors.initials: M
authors.lastname: Ernst
authors.forename: Hartmut
authors.initials: H
authors.lastname: Oschkinat
authors.forename: Ümit
authors.initials: Ü
authors.lastname: Akbey
authors.forename: Niels Chr
authors.initials: NC
authors.lastname: Nielsen
mesh_terms: D003903:Deuterium / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008962:Models, Theoretical
chemical_list: D003903:Deuterium