title: Anti-ageing effects of dentifrices containing anti-oxidative, anti-inflammatory, and anti-bacterial agents (Tomarina®) on gingival collagen degradation in rats.
abstract: OBJECTIVE
  Previous studies have demonstrated the relationship between ageing and oxidative stress. In this study, we examined the effects of topical application of a dentifrice containing anti-oxidative, anti-inflammatory, and anti-bacterial agents (Tomarina®) to the gingival surface on gingival collagen degradation in rats.
  
  
  METHODS
  Fischer 344 male rats (4 or 8 months old) were divided into two groups: experimental group and control group. Tomarina® (the experimental group) or control dentifrice (the control group) was applied 5 days per week for 2 months.
  
  
  RESULTS
  In the control group, gingival collagen density decreased with ageing. In the experimental group, the collagen density did not change with ageing, and was greater than that in the control group at 10 months of age (p < 0.0083). In addition, the control group showed an increase in serum oxidative stress with ageing. The experimental group also showed increased serum oxidative stress, but the value was lower than the control group at 10 months of age (p < 0.0083). Furthermore, low expressions of protein oxidative damage in the periodontal tissue were observed in the experimental group, compared to the control group at 6 months and 10 months.
  
  
  CONCLUSIONS
  These findings indicate that Tomarina® might suppress the effects of ageing on gingival collagen degradation, by decreasing oxidative stress in the rat model.
authors.forename: Irie
authors.initials: I
authors.lastname: Koichiro
authors.forename: Takaaki
authors.initials: T
authors.lastname: Tomofuji
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ekuni
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Endo
authors.forename: Kenta
authors.initials: K
authors.lastname: Kasuyama
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Azuma
authors.forename: Naofumi
authors.initials: N
authors.lastname: Tamaki
authors.forename: Toshiki
authors.initials: T
authors.lastname: Yoneda
authors.forename: Manabu
authors.initials: M
authors.lastname: Morita
mesh_terms: D000375:Aging / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D003094:Collagen / Q000187:drug effects*; D003802:Dentifrices / Q000737:chemistry / Q000494:pharmacology*; D005419:Flavonoids / Q000494:pharmacology; D005881:Gingiva / Q000187:drug effects*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts; D051381:Rats; D011916:Rats, Inbred F344; D017382:Reactive Oxygen Species / Q000097:blood; D018709:Statistics, Nonparametric
chemical_list: D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D003802:Dentifrices; D005419:Flavonoids; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C024070:pycnogenols; D003094:Collagen