title: [The expression of caspase-3, bFGF and MVD in laryngeal squamous cell carcinoma and the relationship among them].
abstract: OBJECTIVE
  To investigate the expression of Caspase-3 and bFGF in laryngeal squamous cell carcinoma and the affection angiogenesis.
  
  
  METHODS
  Immunohistochemistry was used to detect the expression of Caspase-3. bFGF and MVD in 36 cases of para-carcinoma tissue and 67 cases of laryngeal squamous cell carcinoma.
  
  
  RESULTS
  The expression of bFGF in laryngeal squamous cell carcinoma was significantly higher than that in para-carcinoma tissue (P < 0.05), however the expression of Caspase-3 was significantly higher in para-carcinoma tissue than that in Laryngeal squamous cell carcinoma (P < 0.05). In laryngeal carcinoma, the expression of bFGF and Caspase-3 were associated with differentiation, lymph node metastasis and clinical stage (P < 0.05), but was independent of clinical classification,smoking history, sex and gender (P > 0.05). A significantly negative correlation was found between hFGF and Caspase-3 (P < 0.05). The value of MVD in laryngeal squamous cell carcinoma was significantly higher than that in para-carcinoma tissue (P < 0.05), and was significantly associated with lymph node metastasis (P < 0.05), but not associated with age, gender, differentiation, clinical stage, clinical classification or smoking history. The MVD of the tissue with positive expression of bFGF was significantly higher than that with negative expression of bFGF in laryngeal squamous cell carcinoma (P < 0.05). There was no significant difference between the MVI) of the tissue with positive expression of bFGF and that with negative expression of bFGF in laryngeal squamous cell carcinoma (P > 0.05).
  
  
  CONCLUSIONS
  bFGF was positively related to Caspase-3, which might play an important role in the carcinogenesis and development of laryngeal carcinoma by synergic effect.
authors.affiliation: Department of Radiation Oncology, Affiliated Hospital of Chengde Medical College, Chengde, 067000, China. xuqin4463@163.com
authors.forename: Qin
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Department of Otolaryngology, Affiliated Hospital of Chengde Medical College.
authors.forename: Shan
authors.initials: S
authors.lastname: Gao
authors.affiliation: Department of Otolaryngology, Affiliated Hospital of Chengde Medical College.
authors.forename: Ji'na
authors.initials: J
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000098:blood supply* / Q000378:metabolism* / Q000473:pathology; D053148:Caspase 3 / Q000378:metabolism*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D006258:Head and Neck Neoplasms / Q000098:blood supply* / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007822:Laryngeal Neoplasms / Q000098:blood supply* / Q000378:metabolism* / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: D016222:Fibroblast Growth Factor 2; C505407:CASP3 protein, human; D053148:Caspase 3