title: Influence of kinetin on protochlorophyll(ide) accumulation and on the Shibata shift in Raphanus seedlings.
abstract: Radish seedlings (Raphanus sativus L. Saxa Treib) were grown in the dark with or without added kinetin (2 mg/l=9.29 μM). Low-temperature (77°K) fluorescence emission and absorption spectra of etiolated cotyledons were registered at increasing seedling age before and immediately, 30 s and 30 min after one 1-ms flash. Kinetin was found to induce a higher accumulation of the phototransformable protochlorophyll(ide) P657-650 in the etiolated cotyledons, especially from day 6 to day 10 after germination. The amount of the P657-650 protochlorophyll(ide) resynthesized during a 30-min dark period after a 1-ms flash decreased with seedling age. It was smaller in cotyledons from kinetin-treated seedlings at day 6 after germination and at that age only. The ability to perform the Shibata shift decreased with increasing seedling age. In cotyledons from 10- and 13-day-old seedlings, the shift was accomplished to a greater extent when the plants were grown in the presence of kinetin.
authors.affiliation: Laboratory of Photobiology, University of Liège, Sart-Tilman, B-4000, Liège, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Buschmann
authors.forename: C
authors.initials: C
authors.lastname: Sironval