title: Mechanical properties and dual drug delivery application of poly(lactic-co-glycolic acid) scaffolds fabricated with a poly(β-amino ester) porogen.
abstract: Polymeric scaffolds that are biocompatible and biodegradable are widely used for tissue engineering applications. Scaffolds can be further enhanced by enabling the release of one or more drugs to stimulate regeneration or for the treatment of a specific disease or condition. In this study, poly(lactic-co-glycolic acid) (PLGA) microspheres were mixed with poly(β-amino ester) (PBAE) particles to create novel hybrid scaffolds capable of dual release of drug and growth factor. Fast-degrading PBAE particles loaded with the drug ketoprofen acted as porogens that provided a rapid 12h release. The PLGA microspheres were loaded with a growth factor, bone morphogenetic protein 2, and fused together around the porogens to create a slow-degrading matrix that provided sustained release lasting 70days. Drug release was further tailored by varying the amount of porogen added to the scaffold. Bioactivity measurements demonstrated that the scaffold fabrication technique did not damage the drug or protein. The compressive modulus was affected by the amount of porogen added, extending from 50 to 111MPa for loadings from 60 to 40% PBAE, and after 5days of degradation, it decreased to 0.6 to 1.1kPa when the porogen was gone. PLGA containing a quick-degrading porogen can be used to release two drugs while developing a porous microarchitecture for cell ingrowth with in a matrix capable of maintaining a compressive modulus applicable for soft tissue implants.
authors.affiliation: Department of Biomedical Engineering, University of Kentucky, Lexington, KY, USA.
authors.forename: Amanda
authors.initials: A
authors.lastname: Clark
authors.affiliation: Department of Orthopaedic Surgery, University of Kentucky, Lexington, KY, USA; Shriners Hospital for Children, Lexington, KY, USA.
authors.forename: Todd A
authors.initials: TA
authors.lastname: Milbrandt
authors.affiliation: Chemical and Materials Engineering, University of Kentucky, Lexington, KY, USA.
authors.forename: J Zach
authors.initials: JZ
authors.lastname: Hilt
authors.affiliation: Department of Biomedical Engineering, University of Kentucky, Lexington, KY, USA. Electronic address: puleo@uky.edu.
authors.forename: David A
authors.initials: DA
authors.lastname: Puleo
mesh_terms: D001672:Biocompatible Materials / Q000494:pharmacology; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology; D019245:Compressive Strength / Q000187:drug effects; D016503:Drug Delivery Systems; D007660:Ketoprofen / Q000494:pharmacology; D007700:Kinetics; D019344:Lactic Acid / Q000737:chemistry*; D055595:Mechanical Phenomena / Q000187:drug effects; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D054457:Tissue Scaffolds / Q000737:chemistry*; D055114:X-Ray Microtomography
chemical_list: D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D011108:Polymers; C507253:poly(beta-amino ester); D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D007660:Ketoprofen
keywords: Drug delivery; Poly(lactic-co-glycolic acid); Poly(β-amino ester); Porogen; Scaffolds