title: Genetic diversity analysis of mitochondrial DNA control region in artificially propagated Chinese sucker Myxocyprinus asiaticus.
abstract: The genetic diversity of the three major artificially propagated populations of Chinese sucker, an endangered freshwater fish species, was investigated using the sequences of mitochondrial DNA (mtDNA) control regions. Among the 89 individuals tested, 66 variable sites (7.26%) and 10 haplotypes were detected (Haplotype diversity Hd = 0.805, Nucleotide diversity π = 0.0287). In general, genetic diversity was lower in artificially propagated populations than in wild populations. This reduction in genetic diversity may be due to population bottlenecks, genetic drift and human selection. A stepping-stone pattern of gene flow was detected in the populations studied, showing much higher gene flow between neighbouring populations. To increase the genetic diversity, wild lineages should be introduced, and more lineages should be shared among artificially propagated populations.
authors.affiliation: Center for Watershed Ecology, Institute of Life Science, Nanchang University , Nanchang , China .
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wan
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Zhou
authors.forename: Shan
authors.initials: S
authors.lastname: Ouyang
authors.forename: Xiao-Chen
authors.initials: XC
authors.lastname: Huang
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhan
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
authors.forename: Jun
authors.initials: J
authors.lastname: Rong
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Wu
mesh_terms: D000818:Animals; D001947:Breeding / Q000379:methods; D003531:Cypriniformes / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000737:chemistry*; D056727:Endangered Species; D051456:Gene Flow; D040961:Genetic Drift; D014644:Genetic Variation; D006239:Haplotypes; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial
keywords: Artificial propagation; Chinese sucker; control region; genetic diversity; mitochondrial DNA