title: Intravital imaging of donor allogeneic effector and regulatory T cells with host dendritic cells during GVHD.
abstract: Graft-versus-host disease (GVHD) is a systemic inflammatory response due to the recognition of major histocompatibility complex disparity between donor and recipient after hematopoietic stem cell transplantation (HSCT). T-cell activation is critical to the induction of GVHD, and data from our group and others have shown that regulatory T cells (Tregs) prevent GVHD when given at the time of HSCT. Using multiphoton laser scanning microscopy, we examined the single cell dynamics of donor T cells and dendritic cells (DCs) with or without Tregs postallogeneic transplantation. We found that donor conventional T cells (Tcons) spent very little time screening host DCs. Tcons formed stable contacts with DCs very early after transplantation and only increased velocity in the lymph node at 20 hours after transplant. We also observed that Tregs reduced the interaction time between Tcons and DCs, which was dependent on the generation of interleukin 10 by Tregs. Imaging using inducible Tregs showed similar disruption of Tcon-DC contact. Additionally, we found that donor Tregs induce host DC death and down-regulate surface proteins required for donor T-cell activation. These data indicate that Tregs use multiple mechanisms that affect host DC numbers and function to mitigate acute GVHD.
authors.affiliation: Lineberger Comprehensive Cancer Center.
authors.forename: Kaifeng Lisa
authors.initials: KL
authors.lastname: Lin
authors.forename: LeShara M
authors.initials: LM
authors.lastname: Fulton
authors.forename: Matthew
authors.initials: M
authors.lastname: Berginski
authors.forename: Michelle L
authors.initials: ML
authors.lastname: West
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Taylor
authors.forename: Timothy P
authors.initials: TP
authors.lastname: Moran
authors.forename: James M
authors.initials: JM
authors.lastname: Coghill
authors.forename: Bruce R
authors.initials: BR
authors.lastname: Blazar
authors.forename: James E
authors.initials: JE
authors.lastname: Bear
authors.forename: Jonathan S
authors.initials: JS
authors.lastname: Serody
mesh_terms: D000818:Animals; D051940:B7-2 Antigen / Q000378:metabolism; D002450:Cell Communication / Q000276:immunology; D016923:Cell Death / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D004195:Disease Models, Animal; D006086:Graft vs Host Disease / Q000276:immunology* / Q000378:metabolism; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism; D014184:Transplantation, Homologous
chemical_list: D051940:B7-2 Antigen; D018799:Intercellular Adhesion Molecule-1