title: Protein expression-yeast.
abstract: Yeast is an excellent system for the expression of recombinant eukaryotic proteins. Both endogenous and heterologous proteins can be overexpressed in yeast (Phan et al., 2001; Ton and Rao, 2004). Because yeast is easy to manipulate genetically, a strain can be optimized for the expression of a specific protein. Many eukaryotic proteins contain posttranslational modifications that can be performed in yeast but not in bacterial expression systems. In comparison with mammalian cell culture expression systems, growing yeast is both faster and less expensive, and large-scale cultures can be performed using fermentation. While several different yeast expression systems exist, this chapter focuses on the budding yeast Saccharomyces cerevisiae and will briefly describe some options to consider when selecting vectors and tags to be used for protein expression. Throughout this chapter, the expression and purification of yeast eIF3 is shown as an example alongside a general scheme outline.
authors.affiliation: Department of Molecular Biology, Aarhus University, Aarhus, Denmark. Electronic address: klahn@bioxray.au.dk.
authors.forename: Klaus H
authors.initials: KH
authors.lastname: Nielsen
mesh_terms: D002021:Buffers; D002458:Cell Fractionation; D039621:Eukaryotic Initiation Factor-3 / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D015870:Gene Expression; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification
chemical_list: D002021:Buffers; D039621:Eukaryotic Initiation Factor-3; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins
keywords: Growth of yeast cells; High-pressure homogenizer (HPH); Housekeeping promoter; Lysis of yeast cells; Protein expression-yeast; Purification of protein; Tobacco etch virus (TEV)