title: Characteristics of extended-spectrum cephalosporin-resistant Escherichia coli isolated from Swiss and imported poultry meat.
abstract: A worrisome phenomenon is the progressive global spread of Enterobacteriaceae in poultry and chicken meat expressing plasmid-mediated enzymes that inactivate β-lactam antibiotics, suggesting that the food chain might play a role in the epidemiology and the transmission of extended-spectrum cephalosporin-resistant Enterobacteriaceae to humans. The aim of the present study was to further characterize 24 extended-spectrum cephalosporin-resistant Enterobacteriaceae isolated from domestic and imported poultry meat by antibiotic susceptibility testing, identification of the blaESBL/blapAmpC genes, conjugation mating experiments and determination of plasmid incompatibility types, multilocus sequence typing, and analysis of the Escherichia coli phylogenetic groups. On account of their resistance patterns, 21 of the total 24 isolates were classified as multidrug resistant. Eleven isolates carried a blaCMY-2 gene, whereas 13 isolates harbored a blaCTX-M-1 gene. All isolates harbored plasmids that were assigned to 8 of the 18 described plasmid incompatibility groups, the most frequent of which were IncI1, IncFIB, IncB/O, and IncFrepB. The blaESBL/blapAmpC genes were harbored mainly by transferable IncI1 and IncB/O plasmids. Multilocus sequence typing as well as E. coli phylogenetic group typing revealed a high heterogenicity even among different isolates of the same sample.
authors.affiliation: Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Zurich, Switzerland.
authors.forename: H
authors.initials: H
authors.lastname: Abgottspon
authors.affiliation: Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Zurich, Switzerland.
authors.forename: R
authors.initials: R
authors.lastname: Stephan
authors.affiliation: Kantonales Laboratorium Basel-Stadt, Basel, Switzerland.
authors.forename: C
authors.initials: C
authors.lastname: Bagutti
authors.affiliation: Kantonales Laboratorium Basel-Stadt, Basel, Switzerland.
authors.forename: P
authors.initials: P
authors.lastname: Brodmann
authors.affiliation: Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Zurich, Switzerland.
authors.forename: H
authors.initials: H
authors.lastname: Hächler
authors.affiliation: Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Zurich, Switzerland.
authors.forename: K
authors.initials: K
authors.lastname: Zurfluh
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents; D004926:Escherichia coli / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D059022:Food Safety; D006801:Humans; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D010957:Plasmids / Q000235:genetics*; D011202:Poultry Products / Q000382:microbiology*; D013557:Switzerland; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases