title: Introduction of chromosome segment carrying the seed storage protein genes from chromosome 1V of Dasypyrum villosum showed positive effect on bread-making quality of common wheat.
abstract: Development of wheat- D. villosum 1V#4 translocation lines; physically mapping the Glu - V1 and Gli - V1 / Glu - V3 loci; and assess the effects of the introduced Glu - V1 and Gli - V1 / Glu - V3 on wheat bread-making quality. Glu-V1 and Gli-V1/Glu-V3 loci, located in the chromosome 1V of Dasypyrum villosum, were proved to have positive effects on grain quality. However, there are very few reports about the transfer of the D. villosum-derived seed storage protein genes into wheat background by chromosome manipulation. In the present study, a total of six CS-1V#4 introgression lines with different alien-fragment sizes were developed through ionizing radiation of the mature female gametes of CS--D. villosum 1V#4 disomic addition line and confirmed by cytogenetic analysis. Genomic in situ hybridization (GISH), chromosome C-banding, twelve 1V#4-specific EST-STS markers and seed storage protein analysis enabled the cytological physical mapping of Glu-V1 and Gli-V1/Glu-V3 loci to the region of FL 0.50-1.00 of 1V#4S of D. villosum. The Glu-V1 allele of D. villosum was Glu-V1a and its coded protein was V71 subunit. Quality analysis indicated that Glu-V1a together with Gli-V1/Glu-V3 loci showed a positive effect on protein content, Zeleny sedimentation value and the rheological characteristics of wheat flour dough. In addition, the positive effect could be maintained when specific Glu-V1 and Gli-V1/Glu-V3 loci were transferred to the wheat genetic background as in the case of T1V#4S-6BS · 6BL, T1V#4S · 1BL and T1V#4S · 1DS translocation lines. These results showed that the chromosome segment carrying the Glu-V1 and Gli-V1/Glu-V3 loci in 1V#4S of D. villosum had positive effect on bread-making quality, and the T1V#4S-6BS · 6BL and T1V#4S · 1BL translocation lines could be useful germplasms for bread wheat improvement. The developed 1V#4S-specific molecular markers could be used to rapidly identify and trace the alien chromatin of 1V#4S in wheat background.
authors.affiliation: National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Ruiqi
authors.forename: Zhang
authors.initials: Z
authors.lastname: Mingyi
authors.forename: Wang
authors.initials: W
authors.lastname: Xiue
authors.forename: Chen
authors.initials: C
authors.lastname: Peidu
mesh_terms: D000483:Alleles; D001939:Bread; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics*; D005433:Flour; D017343:Genes, Plant; D056426:Genetic Loci; D006109:Poaceae / Q000235:genetics*; D055314:Seed Storage Proteins / Q000235:genetics*; D012639:Seeds / Q000235:genetics; D014178:Translocation, Genetic; D014908:Triticum / Q000235:genetics*
chemical_list: D055314:Seed Storage Proteins