title: Proteomic analysis in caged Mediterranean crab (Carcinus maenas) and chemical contaminant exposure in Téboulba Harbour, Tunisia.
abstract: This study uses proteomics approach to assess the toxic effects of contaminants in the Mediterranean crab (Carcinus maenas) after transplantation into Téboulba fishing harbour. High levels of aliphatic and aromatic hydrocarbons were detected in sediments. Although their effects on vertebrates are well described, little is known about their early biological effects in marine invertebrates under realistic conditions. Protein expression profiles of crabs caged for 15, 30 and 60 days were compared to unexposed animals. Nineteen proteins with significant expression differences were identified by capLC-µESI-IT MS/MS and homology search on databases. Differentially expressed proteins were assigned to five different categories of biological function including: (1) chitin catabolism, (2) proteolysis, (3) exoskeleton biosynthesis, (4) protein folding and stress response, and (5) transport. The proteins showing major expression changes in C. maenas after different caging times may be considered as novel molecular biomarkers for effectively biomonitoring aquatic environment contamination.
authors.affiliation: Laboratory of Biochemical and Environmental Toxicology, Higher Institute of Agriculture, Chott-Mariem, 4042-Sousse, Tunisia. Electronic address: jebali.jamel@laposte.net.
authors.forename: Jamel
authors.initials: J
authors.lastname: Jebali
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Córdoba, Severo Ochoa Building, Rabanales Campus, Highway A4 Km 396a, 14071-Córdoba, Spain; Maimonides Institute for Research in Biomedicine of Córdoba, Reina Sofía University Hospital, University of Córdoba, 14071-Córdoba, Spain.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Chicano-Gálvez
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Córdoba, Severo Ochoa Building, Rabanales Campus, Highway A4 Km 396a, 14071-Córdoba, Spain.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Fernández-Cisnal
authors.affiliation: Laboratory of Biochemical and Environmental Toxicology, Higher Institute of Agriculture, Chott-Mariem, 4042-Sousse, Tunisia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Banni
authors.affiliation: Chemical Laboratory, Higher Institute of Marine Sciences and Technology, La Goulette Center, 2060 Tunis, Tunisia.
authors.forename: Lassaad
authors.initials: L
authors.lastname: Chouba
authors.affiliation: Laboratory of Biochemical and Environmental Toxicology, Higher Institute of Agriculture, Chott-Mariem, 4042-Sousse, Tunisia.
authors.forename: Hamadi
authors.initials: H
authors.lastname: Boussetta
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Córdoba, Severo Ochoa Building, Rabanales Campus, Highway A4 Km 396a, 14071-Córdoba, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: López-Barea
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Córdoba, Severo Ochoa Building, Rabanales Campus, Highway A4 Km 396a, 14071-Córdoba, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Alhama
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D003386:Brachyura / Q000187:drug effects*; D004781:Environmental Exposure; D004784:Environmental Monitoring; D040901:Proteomics; D053719:Tandem Mass Spectrometry; D014416:Tunisia; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity
chemical_list: D015415:Biomarkers; D014874:Water Pollutants, Chemical
keywords: Carcinus maenas; Hydrocarbons; Metals; Proteomics; Transplantation