title: Imaging of molecular surface dynamics in brain slices using single-particle tracking.
abstract: Organization of signalling molecules in biological membranes is crucial for cellular communication. Many receptors, ion channels and cell adhesion molecules are associated with proteins important for their trafficking, surface localization or function. These complexes are embedded in a lipid environment of varying composition. Binding affinities and stoichiometry of such complexes were so far experimentally accessible only in isolated systems or monolayers of cell culture. Visualization of molecular dynamics within signalling complexes and their correlation to specialized membrane compartments demand high temporal and spatial resolution and has been difficult to demonstrate in complex tissue like brain slices. Here we demonstrate the feasibility of single-particle tracking (SPT) in organotypic brain slices to measure molecular dynamics of lipids and transmembrane proteins in correlation to synaptic membrane compartments. This method will provide important information about the dynamics and organization of surface molecules in the complex environment of neuronal networks within brain slices.
authors.affiliation: 1] Research Group Molecular Physiology, Leibniz-Institute of Neurobiology, Brenneckestrasse 6, D-39118 Magdeburg, Germany [2] Institute of Anatomy and Molecular Neurobiology, Westfälische Wilhelms-University, Vesaliusweg 2-4, D-48149 Münster, Germany [3].
authors.forename: B
authors.initials: B
authors.lastname: Biermann
authors.affiliation: 1] Research Group Molecular Physiology, Leibniz-Institute of Neurobiology, Brenneckestrasse 6, D-39118 Magdeburg, Germany [2].
authors.forename: S
authors.initials: S
authors.lastname: Sokoll
authors.affiliation: Research Group Molecular Physiology, Leibniz-Institute of Neurobiology, Brenneckestrasse 6, D-39118 Magdeburg, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Klueva
authors.affiliation: Institute of Anatomy and Molecular Neurobiology, Westfälische Wilhelms-University, Vesaliusweg 2-4, D-48149 Münster, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Missler
authors.affiliation: Center for Molecular Neurobiology (ZMNH), University Medical Center Hamburg-Eppendorf, Falkenried 94, D-20251 Hamburg, Germany.
authors.forename: J S
authors.initials: JS
authors.lastname: Wiegert
authors.affiliation: 1] University of Bordeaux, Interdisciplinary Institute for Neuroscience, F-33000 Bordeaux, France [2] CNRS UMR 5297, F-33000 Bordeaux, France.
authors.forename: J-B
authors.initials: JB
authors.lastname: Sibarita
authors.affiliation: Research Group Molecular Physiology, Leibniz-Institute of Neurobiology, Brenneckestrasse 6, D-39118 Magdeburg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Heine
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism*; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D050356:Lipid Metabolism; D008563:Membrane Lipids / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008856:Microscopy, Fluorescence; D056004:Molecular Dynamics Simulation; D015394:Molecular Structure; D045663:Quantum Dots; D051381:Rats
chemical_list: D008563:Membrane Lipids; D008565:Membrane Proteins