title: Recent progress in abasic site-binding small molecules for detecting single-base mutations in DNA.
abstract: This review addresses our recent efforts to design AP site-binding small ligands for SNP (single-nucleotide polymorphisms) typing. First, we present a surface plasmon resonance (SPR) biosensor carrying a derivative of 3,5-diaminopyrazines. Comparison with a bulk assay based on 3,5-diaminopyrazines-DNA binding shows that the immobilization of 3,5-diaminopyrazines on the SPR sensor allows a more sensitive detection of the target DNA, and binding selectivity can be tuned by controlling salt concentrations of the sample solutions. We also present a ratiometric fluorescent probe, in which an environmentally sensitive fluorescent dye, a benzofurazan derivative, is linked through an alkyl spacer to a 2-amino-1,8-naphthyridine derivative. The binding and sensing properties of these systems are discussed as a basis for the advanced design of DNA-binding small molecules for gene detection.
authors.affiliation: Department of Chemistry, Graduate School of Science, Tohoku University.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Nishizawa
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Sato
authors.forename: Norio
authors.initials: N
authors.lastname: Teramae
mesh_terms: D001665:Binding Sites; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D005456:Fluorescent Dyes / Q000737:chemistry; D008024:Ligands; D009154:Mutation; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011719:Pyrazines / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D008024:Ligands; D011719:Pyrazines; D004247:DNA