title: Influence of bacteriophage P22 on the inflammatory mediator gene expression in chicken macrophage HD11 cells infected with Salmonella Typhimurium.
abstract: This study was designed to evaluate the effects of bacteriophage on the intracellular survival and immune mediator gene expression in chicken macrophage-like HD11 cells. The invasive ability and intracellular survival of Salmonella Typhimurium (ST(P22-) ) and lysogenic S. Typhimurium (ST(P22+) ) in HD11 cells were evaluated at 37 °C for 24 h postinfection (hpi). The expression of inflammatory mediator genes was determined in ST(P22-) - and ST(P22+) -infected HD11 cells treated with and without bacteriophage P22 at 1 and 24 hpi using quantitative RT-PCR. The ability of ST(P22-) and ST(P22+) to invade HD11 cells was significantly decreased by bacteriophage P22 at 1 hpi. The numbers of intracellular ST(P22-) and ST(P22+) were significantly decreased from 2.39 to 1.62 CFU cm(-2) and from 3.40 to 1.72 CFU cm(-2) in HD11 cells treated with bacteriophage P22, respectively, at 24 hpi. The enhanced expression of inflammatory mediators was observed in ST(P22-) - and ST(P22+) -infected HD11 cells treated with and without bacteriophage P22. These results suggest that the application of bacteriophage could be an effective way to control the intracellular infection.
authors.affiliation: Department of Medical Biomaterials Engineering, Kangwon National University, Chuncheon, Gangwon, South Korea.
authors.forename: Juhee
authors.initials: J
authors.lastname: Ahn
authors.forename: Debabrata
authors.initials: D
authors.lastname: Biswas
mesh_terms: D000818:Animals; D017100:Bacteriophage P22 / Q000502:physiology*; D001691:Biological Therapy / Q000662:veterinary*; D002460:Cell Line; D002645:Chickens; D016207:Cytokines / Q000235:genetics* / Q000276:immunology; D018836:Inflammation Mediators / Q000276:immunology; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology / Q000821:virology; D008969:Molecular Sequence Data; D011201:Poultry Diseases / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000628:therapy*; D012481:Salmonella Infections, Animal / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000628:therapy*; D012486:Salmonella typhimurium / Q000502:physiology / Q000821:virology*
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators
keywords: HD11 cell; Salmonella Typhimurium; bacteriophage; inflammatory mediator; lysogeny; macrophage