title: Baicalin inhibits high-mobility group box 1 release and improves survival in experimental sepsis.
abstract: Baicalin, as the main active ingredient of the root from Scutellaria, is usually used in the treatment of inflammatory diseases. In our study, we found that baicalin improved survival in septic mice in vivo and attenuated high-mobility group box 1 (HMGB1) and cytokine release from macrophages in vitro. The experiments in vitro showed that baicalin inhibited both viability of macrophages and the cell's secretion of HMGB1, tumor necrosis factor α, interleukin 6 (IL-6), and IL-1β induced by lipopolysaccharide. Meanwhile, experiments in vitro also indicated that baicalin did not affect the transcription and translation of HMGB1 but inhibit the cytoplasmic translocation of HMGB1 induced by lipopolysaccharide. We found that baicalin improved survival and tissue injury of septic mice in vivo. It also decreased serum HMGB1, tumor necrosis factor α, IL-6, and IL-1β in septic mice. In conclusion, baicalin inhibits the release of HMGB1 from macrophages and may be a potential therapeutic strategy for sepsis-related diseases.
authors.affiliation: *Department of nephrology, Tianjin Third Central Hospital; and †Department of pharmacology, Institute of Acute Abdominal Diseases, Tianjin Nankai Hospital, Tianjin, China.
authors.forename: Hongling
authors.initials: H
authors.lastname: Wang
authors.forename: Daquan
authors.initials: D
authors.lastname: Liu
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000097:blood / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005419:Flavonoids / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D024243:HMGB1 Protein / Q000097:blood / Q000378:metabolism*; D018836:Inflammation Mediators / Q000097:blood / Q000378:metabolism; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000494:pharmacology; D008099:Liver / Q000473:pathology; D008168:Lung / Q000473:pathology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018805:Sepsis / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D016019:Survival Analysis; D014178:Translocation, Genetic / Q000187:drug effects
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D016207:Cytokines; D005419:Flavonoids; D024243:HMGB1 Protein; C582808:HMGB1 protein, mouse; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; C038044:baicalin