title: Thermus 2S from Thai hot springs: isolation and immobilization.
abstract: Three strains of thermophilic bacteria producing extracellular protease have been isolated from hot springs in Chiang Mai, Thailand. The bacterium producing the highest amount of protease has been selected and identified as belonging to the genusThermus, and is tentatively calledThermus 2S. The isolate is a Gram-negative, rod shaped bacterium. It exhibited maximum growth around 60°C at pH 7. Entrapment of the microbial cells in calcium alginate maintained the cell viability. Protease production from immobilized cells using 2 g wet cells per 10 ml 3% (w/v) sodium alginate was higher than that from a free-cell system using 2% inoculum.
authors.affiliation: Department of Chemistry, Faculty of Science, Chiang Mai University, 50002, Chiang Mai, Thailand.
authors.forename: P
authors.initials: P
authors.lastname: Kanasawud
authors.forename: S
authors.initials: S
authors.lastname: Teeyapan
authors.forename: S
authors.initials: S
authors.lastname: Lumyong
authors.forename: O
authors.initials: O
authors.lastname: Holst
authors.forename: B
authors.initials: B
authors.lastname: Mattiasson