title: Protective effect of selenomethionine on aflatoxin B1-induced oxidative stress in MDCK cells.
abstract: AFB1 is a mycotoxin which exerts their cytotoxicity through increasing oxidative damage in target organ. Kidney is one of target organs vulnerable to damage caused by AFB1. In this study, Madin-Darby canine kidney (MDCK) cells were used to evaluate the AFB1-induced cell damage by the MTT assay. The results revealed that the toxic effect of AFB1 on MDCK cells is both dose and time dependent. Half maximal toxic concentration (IC50) was noted at 0.25 μg/ml of AFB1. Further, protective effect of six different concentrations (0.2, 0.8, 1, 2, 4, and 8 μM) of selenomethionine (SeMet) was observed against 0.25 μg/ml of AFB1-induced damage. The results showed that 0.25 μg/ml of AFB1 caused significant increase in oxidative stress, which was demonstrated by significant increase of malondialdehyde (MDA) level, reduction of intracellular GSH level, as well as GPX1 activity and mRNA level in MDCK cells when compared with control. SeMet protected the cells from AFB1-induced oxidative damage in a dose-dependant manner. Good protection could be achieved between 1 and 4 μM of concentration. Amid this range, MDA level significantly decreased while intracellular GSH level and GPX1 activity in addition to mRNA level significantly increased. Moreover, cell viability was significantly improved. It could be concluded that SeMet is a potential antioxidative agent to alleviate AFB1-induced oxidative stress.
authors.affiliation: Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Fahmida
authors.initials: F
authors.lastname: Parveen
authors.forename: Zaheer Ahmed
authors.initials: ZA
authors.lastname: Nizamani
authors.forename: Fang
authors.initials: F
authors.lastname: Gan
authors.forename: Xingxiang
authors.initials: X
authors.lastname: Chen
authors.forename: Xiuli
authors.initials: X
authors.lastname: Shi
authors.forename: Shahnawaz
authors.initials: S
authors.lastname: Kumbhar
authors.forename: Alam
authors.initials: A
authors.lastname: Zeb
authors.forename: Kehe
authors.initials: K
authors.lastname: Huang
mesh_terms: D016604:Aflatoxin B1 / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D061985:Madin Darby Canine Kidney Cells; D018384:Oxidative Stress / Q000187:drug effects*; D012645:Selenomethionine / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D012645:Selenomethionine; D016604:Aflatoxin B1