title: Adaptive reprogramming of the breast cancer kinome.
abstract: Our understanding of cancer has grown considerably with recent advances in high-throughput genome and transcriptome sequencing, but techniques to comprehensively analyze protein activity are still in development. Methods to quantitatively measure the activation of signaling pathways within tumors at baseline and following therapeutic intervention will prove critical to the design of proper treatment regimens. Focusing on breast cancer, we present such a method to understand kinase signaling using multiplexed kinase inhibitor beads coupled with mass spectrometry (MIB/MS).
authors.affiliation: Department of Pharmacology, Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA.
authors.forename: T J
authors.initials: TJ
authors.lastname: Stuhlmiller
authors.affiliation: Department of Medicine, Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA.
authors.forename: H S
authors.initials: HS
authors.lastname: Earp
authors.affiliation: Department of Pharmacology, Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA.
authors.forename: G L
authors.initials: GL
authors.lastname: Johnson
mesh_terms: D000222:Adaptation, Physiological; D001943:Breast Neoplasms / Q000473:pathology* / Q000628:therapy; D005260:Female; D020869:Gene Expression Profiling; D023281:Genomics / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D015398:Signal Transduction / Q000502:physiology; D059467:Transcriptome
chemical_list: D047428:Protein Kinase Inhibitors