title: Effect of cold working on biocompatibility of Ni-free high nitrogen austenitic stainless steels using Dalton's Lymphoma cell line.
abstract: The aims of the present work are to explore the effect of cold working on in-vitro biocompatibility of indigenized low cost Ni-free nitrogen containing austenitic stainless steels (HNSs) and to compare it with conventionally used biomedical grade, i.e. AISI 316L and 316LVM, using Dalton's Lymphoma (DL) cell line. The MTT assay [3-(4,5-dimethythiazol 2-yl)-2,5-diphenyltetrazolium bromide] was performed on DL cell line for cytotoxicity evaluation and cell adhesion test. As a result, it was observed that the HNS had higher cell proliferation and cell growth and it increases by increasing nitrogen content and degree of cold working. The surface wettability of the alloys was also investigated by water contact angle measurements. The value of contact angles was found to decrease with increase in nitrogen content and degree of cold working. This indicates that the hydrophilic character increases with increasing nitrogen content and degree of cold working which further attributed to enhance the surface free energy (SFE) which would be conducive to cell adhesion which in turn increases the cell proliferation.
authors.affiliation: Centre of Advanced Study, Department of Metallurgical Engineering, Indian Institute of Technology (Banaras Hindu University) Varanasi 221005, Uttar Pradesh, India.
authors.forename: Mohd
authors.initials: M
authors.lastname: Talha
authors.affiliation: Centre of Advanced Study, Department of Zoology, Banaras Hindu University, Varanasi 221005, Uttar Pradesh, India.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Centre of Advanced Study, Department of Metallurgical Engineering, Indian Institute of Technology (Banaras Hindu University) Varanasi 221005, Uttar Pradesh, India.
authors.forename: C K
authors.initials: CK
authors.lastname: Behera
authors.affiliation: Centre of Advanced Study, Department of Metallurgical Engineering, Indian Institute of Technology (Banaras Hindu University) Varanasi 221005, Uttar Pradesh, India. Electronic address: opsinha.met@itbhu.ac.in.
authors.forename: O P
authors.initials: OP
authors.lastname: Sinha
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003080:Cold Temperature; D006244:Hardness; D006801:Humans; D008223:Lymphoma / Q000473:pathology* / Q000503:physiopathology*; D008422:Materials Testing; D009532:Nickel / Q000737:chemistry / Q000302:isolation & purification; D009584:Nitrogen; D013193:Stainless Steel / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D001672:Biocompatible Materials; C426023:austenitic steel; D013193:Stainless Steel; D009532:Nickel; D009584:Nitrogen
keywords: 316LVM; Cytotoxicity evaluation; HNS