title: The complete mitochondrial genome of Taiwanese spined loach Cobitis cf. sinensis (Teleostei: Cobitidae).
abstract: In this study, the complete mitogenome sequence of Taiwanese spined loach, Cobitis cf. sinensis Sauvage and Dabry de Thiersant (Teleostei: Cobitidae) from northern Taiwan has been amplified and sequenced by employing long polymerase chain reaction method. The mitogenome, consisting of 16,553 bp, had the typical vertebrate mitochondrial gene arrangement, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a non-coding control region (CR). CR of 914 bp length is located between tRNAPro and tRNAPhe. The overall base composition of the loach is 29.9% for A, 25.9% for C, 27.8% for T and 16.3% for G, with a slight AT bias of 57.7%. The complete mitogenome may provide rather essential and important DNA molecular data for further phylogenetic analysis for not only congeneric species but also higher different taxa of the loaches.
authors.affiliation: a Institute of Marine Biology, National Taiwan Ocean University , Keelung , Taiwan , ROC.
authors.forename: I-Shiung
authors.initials: IS
authors.lastname: Chen
authors.affiliation: a Institute of Marine Biology, National Taiwan Ocean University , Keelung , Taiwan , ROC.
authors.forename: Chih-Wen
authors.initials: CW
authors.lastname: Wang
mesh_terms: D000818:Animals; D001483:Base Sequence; D003531:Cypriniformes / Q000145:classification / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics; D054629:Genome, Mitochondrial; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics
chemical_list: D004272:DNA, Mitochondrial; D012343:RNA, Transfer
keywords: Cobitis cf. sinensis; Taiwanese spined loach; mitogenome