title: Molecular characterization of Saudi local chicken strains using mitochondrial DNA markers.
abstract: The current study was carried out to investigate and estimate the genetic diversity of native breeds based on cytochrome b (cyt-b) gene of mitochondrial DNA information. The obtained sequences of cyt-b gene segment have TAA as a stop codon at 488 position with no insertions or deletion in all individuals of both native chicken strains. The blast results showed that no variation was found among individuals within both native chicken strains, but when a comparison was established among them and other species of genus Gallus the variation is exploring, additionally many mutant sites were detected as single nucleotide polymorphisms (SNPs) in different sites. The phylogenetic trees exhibited three different groups. The results revealed that the native chicken strains were closely related to the cluster of Gallus gallus and subspecies of Gallus, suggesting that they may be separated from the same origin. According to this result and previously studies, the native chicken strains are genetically closer to Gallus gallus and it could be successfully distinguished from the other wild types of Gallus chicken based on cyt-b gene information. We recommended that the governmental concerns for native chicken strain should be enhanced to screen its genetic structure for large scale in the Kingdom of Saudi Arabia.
authors.affiliation: Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University , Jeddah , Kingdom of Saudi Arabia .
authors.forename: H A
authors.initials: HA
authors.lastname: Yacoub
authors.forename: H A I
authors.initials: HA
authors.lastname: Ramadan
authors.forename: Nabih A
authors.initials: NA
authors.lastname: Baeshen
authors.forename: Mahmoud Abdel
authors.initials: MA
authors.lastname: Sadek
authors.forename: M E
authors.initials: ME
authors.lastname: Abou Alsoud
mesh_terms: D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics*; D045303:Cytochromes b / Q000737:chemistry / Q000235:genetics; D004272:DNA, Mitochondrial / Q000737:chemistry*; D005819:Genetic Markers; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D012529:Saudi Arabia; D016415:Sequence Alignment; D013045:Species Specificity
chemical_list: D004272:DNA, Mitochondrial; D005819:Genetic Markers; D045303:Cytochromes b
keywords: Characterization; chicken; markers; mitochondrial DNA; molecular; native