title: Double stranded aptamer-anchored reduced graphene oxide as target-specific nano detector.
abstract: Here, we report a double-stranded, dual-anchored, fluorescent aptamer on reduced graphene oxide (rGO) for the sensitive, selective, and speedy detection of a target protein in biological samples. This nano detector is composed of a target protein-specific fluorescent aptamer with BHQ1 as one anchoring moiety that forms double-stranded sequences with a complementary oligonucleotide sequence with BHQ1 as the other anchoring moiety, anchored to rGO nanosheets. The double-stranded and dual-anchored aptamer on rGO nanosheets (DAGO) exhibited 7.3-fold higher fluorescence intensities compared to a single-stranded, single-anchored fluorescent aptamer on rGO. As a model target protein, interferon-γ was used. DAGO detected the target protein, with linearity over a five-orders-of-magnitude concentration range (0.1 ng/ml-10 μg/ml) in buffer and human serum. DAGO was highly specific for the target protein, exhibiting little changes in fluorescence intensity in response to the non-target proteins, interleukin-2 and tumor necrosis factor-α. Moreover, DAGO allowed rapid quantification of the target protein in human immunodeficiency virus-positive patient serum samples. DAGO-based detection was complete in less than 10 min. Our results indicate that the DAGO provides new opportunities for the rapid and specific detection of target proteins in biological samples and could be widely applied to quantitate various target proteins by replacing the aptamer sequences.
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Mi-Gyeong
authors.initials: MG
authors.lastname: Kim
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Yuna
authors.initials: Y
authors.lastname: Shon
authors.affiliation: Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Jaiwoo
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Youngro
authors.initials: Y
authors.lastname: Byun
authors.affiliation: Division of AIDS, Center for Immunology and Pathology, Korea National Institute of Health, Osong, Chungcheongbuk-do 363-951, Republic of Korea.
authors.forename: Byeong-Sun
authors.initials: BS
authors.lastname: Choi
authors.affiliation: Department of Bio-industrial Technologies, College of Animal Bioscience and Technology, Konkuk University, Seoul 143-701, Republic of Korea. Electronic address: kimera@konkuk.ac.kr.
authors.forename: Young Bong
authors.initials: YB
authors.lastname: Kim
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Republic of Korea. Electronic address: ohyk@snu.ac.kr.
authors.forename: Yu-Kyoung
authors.initials: YK
authors.lastname: Oh
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D002021:Buffers; D005453:Fluorescence; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D012015:Reference Standards; D044967:Serum / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D002021:Buffers; D010087:Oxides; D011506:Proteins; D006108:Graphite
keywords: Aptamers; Graphene nanosheets; Nano detectors; Rapid detection; Target-sensitivity; Target-specificity