title: Hydrogen sulfide alleviates postharvest senescence of broccoli by modulating antioxidant defense and senescence-related gene expression.
abstract: Accumulating evidence has shown that hydrogen sulfide (H₂S) acts as a signaling regulator in plants. Here we show that H₂S delays the postharvest senescence of broccoli in a dose-dependent manner. H₂S maintains higher levels of metabolites, such as carotenoids, anthocyanin, and ascorbate, and reduces the accumulation of malondialdehyde, H₂O₂, and the superoxide anion. Further investigations showed that H₂S sustained higher activities of guaiacol peroxidase, ascorbate peroxidase, catalase, and glutathione reductase and lower activities of lipoxygenase, polyphenol oxidase, phenylalanine ammonia lyase, and protease than those of water control. Moreover, the expression of the chlorophyll degradation related genes BoSGR, BoCLH2, BoPaO, BoRCCR, as well as cysteine protease BoCP1 and lipoxygenase gene BoLOX1, was down-regulated in postharvest broccoli treated with H₂S. The functions of H₂S on the senescence of other vegetables and fruits suggest its universal role acting as a senescence regulator.
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology , Hefei 230009, China.
authors.forename: Shi-Ping
authors.initials: SP
authors.lastname: Li
authors.forename: Kang-Di
authors.initials: KD
authors.lastname: Hu
authors.forename: Lan-Ying
authors.initials: LY
authors.lastname: Hu
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Li
authors.forename: An-Min
authors.initials: AM
authors.lastname: Jiang
authors.forename: Fang
authors.initials: F
authors.lastname: Xiao
authors.forename: Yi
authors.initials: Y
authors.lastname: Han
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Liu
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D060387:Ascorbate Peroxidases / Q000235:genetics / Q000378:metabolism; D001937:Brassica / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D016922:Cellular Senescence / Q000187:drug effects; D002734:Chlorophyll / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D008315:Malondialdehyde / Q000378:metabolism; D009195:Peroxidase; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010940:Plant Proteins; D002734:Chlorophyll; D008315:Malondialdehyde; C121048:guaiacol peroxidase; D060387:Ascorbate Peroxidases; D002374:Catalase; D009195:Peroxidase; D006862:Hydrogen Sulfide