title: In vitro and in vivo studies on the transport of PEGylated silica nanoparticles across the blood-brain barrier.
abstract: Transport of PEGylated silica nanoparticles (PSiNPs) with diameters of 100, 50, and 25 nm across the blood-brain barrier (BBB) was evaluated using an in vitro BBB model based on mouse cerebral endothelial cells (bEnd.3) cultured on transwell inserts within a chamber. In vivo animal experiments were further performed by noninvasive in vivo imaging and ex vivo optical imaging after injection via carotid artery. Confocal fluorescence studies were carried out to evaluate the uptake of PSiNPs by brain endothelial cells. The results showed that PSiNPs can traverse the BBB in vitro and in vivo. The transport efficiency of PSiNPs across BBB was found to be size-dependent, with increased particle size resulting in decreased efficiency. This work points to the potential application of small sized silica nanoparticles in brain imaging or drug delivery.
authors.affiliation: Affiliated Chenggong Hospital, State Key Laboratory of Physical Chemistry of Solid Surfaces, the Key Laboratory for Chemical Biology of Fujian Province, The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University , Xiamen 361005, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.forename: Bingqian
authors.initials: B
authors.lastname: Lin
authors.forename: Wei
authors.initials: W
authors.lastname: Shao
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhu
authors.forename: Tianhai
authors.initials: T
authors.lastname: Ji
authors.forename: Chaoyong
authors.initials: C
authors.lastname: Yang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D001812:Blood-Brain Barrier / Q000187:drug effects / Q000378:metabolism*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002460:Cell Line; D017097:Electric Impedance; D004705:Endocytosis / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D008807:Mice, Inbred BALB C; D008954:Models, Biological; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D055672:Static Electricity
chemical_list: D001672:Biocompatible Materials; D011092:Polyethylene Glycols; D012822:Silicon Dioxide