title: Evaluation of polymeric adsorbent resins for efficient detoxification of liquor generated during acid pretreatment of lignocellulosic biomass.
abstract: Production of fuel ethanol from lignocellulosic biomass conventionally includes biomass pretreatment, hydrolysis, and fermentation. The liquor generated during dilute acid pretreatment of biomass contains considerable quantities of pentose sugars as well as various degradation products of sugars and lignin, like furfural, hydroxymethyl furfural (HMF), organic acids, aldehydes and others, which are known to be inhibitory for microbial growth. This pentose rich liquor is a potent resource which can be used to produce alcohol or other value added metabolites by microbial fermentation. However, the presence of these inhibitory compounds is a major hindrance and their removal is essential for efficient utilization of this byproduct stream. In the present work, the polymeric adsorbent resins, XAD-4, XAD-7 and XAD-16 were evaluated for their ability to adsorb fermentation inhibitors like furfural and HMF from the acid pretreated liquor. These resins could remove 55-75% of furfural and 100% of HMF and more than 90% sugar remained un-adsorbed in the pretreated liquor. Desorption of furfural from stationary phase was evaluated by using ethanol and hot water. The results suggest that these polymeric resins may be used for detoxification of acid pretreatment liquor with selective removal of sugar degradation products without affecting the sugar content in the solution.
authors.affiliation: Centre for Biofuels, CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum 695 019, India.
authors.forename: Soolamkandath Variem
authors.initials: SV
authors.lastname: Sandhya
authors.affiliation: Centre for Biofuels, CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum 695 019, India.
authors.forename: Kumar
authors.initials: K
authors.lastname: Kiran
authors.affiliation: Centre for Biofuels, CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum 695 019, India.
authors.forename: Mathiyazhakan
authors.initials: M
authors.lastname: Kuttiraja
authors.affiliation: Centre for Biofuels, CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum 695 019, India.
authors.forename: Varghese Elizabeth
authors.initials: VE
authors.lastname: Preeti
authors.affiliation: Centre for Biofuels, CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum 695 019, India.
authors.forename: Raveendran
authors.initials: R
authors.lastname: Sindhu
authors.affiliation: Centre for Biofuels, CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum 695 019, India.
authors.forename: Sankar
authors.initials: S
authors.lastname: Vani
authors.affiliation: Centre for Biofuels, CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum 695 019, India.
authors.forename: Sukumaran Rajeev
authors.initials: SR
authors.lastname: Kumar
authors.affiliation: Centre for Biofuels, CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum 695 019, India.
authors.forename: Ashok
authors.initials: A
authors.lastname: Pandey
authors.affiliation: Centre for Biofuels, CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum 695 019, India.
authors.forename: Parameswaran
authors.initials: P
authors.lastname: Binod
mesh_terms: D000143:Acids / Q000737:chemistry*; D000327:Adsorption; D018533:Biomass; D005285:Fermentation; D008031:Lignin / Q000737:chemistry* / Q000378:metabolism; D011108:Polymers / Q000737:chemistry*
chemical_list: D000143:Acids; D011108:Polymers; C036909:lignocellulose; D008031:Lignin