title: Engineering of a Pluronic F127 functionalized magnetite/graphene nanohybrid for chemophototherapy.
abstract: In this study, a multifunctional graphene based nanohybrid, termed as GN/Fe3O4/PF127, is engineered via a facile one-pot process consisting of simultaneous reduction of graphene oxide/Fe3O4 and subsequent assembly of Pluronic F127 (PF127) onto graphene nanosheets (GNs). The unique aromatic and planar structure of GNs allows the attachment of multiple functional components including MRI contrast agent (Fe3O4 nanoparticles) and an aromatic anticancer drug like doxorubicin (DOX), as well as PF127 coating which imparts physiological dispersivity and stability to the nanohybrid. The successful assembly process is revealed by TEM observation, size and FITR monitoring. In contrast with the primitive graphene or its oxide derivative, the resulting GN/Fe3O4/PF127 nanohybrids have shown high biological dispersion and MRI effect for diagnosis due to the incorporation of superparamagnetic Fe3O4 nanoparticles without evident cytotoxicity. Moreover, the GN/Fe3O4/PF127 nanohybrid exhibits a photothermal effect due to the considerable optical absorption in the near-infrared region of GNs. The GN/Fe3O4/PF127 nanohybrid could be a further platform for chemophototherapy assisted by the therapeutic DOX. Cellular toxicity assays indicated that the DOX-loaded GN/Fe3O4/PF127 nanohybrid showed a remarkable cytotoxicity to HeLa cells and the cytotoxic effect was intensified when subjected to photoirradiation. Confocal laser scanning microscopy (CLSM) and flow cytometric analysis (FCAS) revealed that the nanohybrid could be easily uptaken into HeLa cells.
authors.affiliation: The Institute for Biomedical Engineering and Nano Science, Tongji University School of Medicine, Shanghai 200092, People's Republic of China.
authors.forename: Yongyong
authors.initials: Y
authors.lastname: Li
authors.forename: Jiaqiang
authors.initials: J
authors.lastname: Liu
authors.forename: Haiqing
authors.initials: H
authors.lastname: Dong
authors.forename: Guangzhen
authors.initials: G
authors.lastname: Liu
authors.forename: Haiqing
authors.initials: H
authors.lastname: Hu
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage; D002470:Cell Survival / Q000187:drug effects; D003287:Contrast Media / Q000737:chemistry; D004317:Doxorubicin / Q000008:administration & dosage*; D004358:Drug Therapy / Q000379:methods*; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D005434:Flow Cytometry; D006108:Graphite / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008279:Magnetic Resonance Imaging; D008422:Materials Testing; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites / Q000737:chemistry*; D050997:Nanomedicine / Q000379:methods; D010789:Phototherapy / Q000379:methods*; D020442:Poloxamer / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000970:Antineoplastic Agents; D003287:Contrast Media; D020442:Poloxamer; D006108:Graphite; D004317:Doxorubicin; D052203:Ferrosoferric Oxide