title: Distribution of Clostridium perfringens isolates from piglets in South Korea.
abstract: Clostridium perfringens causes various digestive system disease symptoms in pigs. In the present study, 11 C. perfringens isolates were obtained from diarrheic piglets and 18 from healthy piglets. All of the C. perfringens isolates were shown to be type A using a multiplex PCR assay. The β2 toxin gene was detected in 27/29 C. perfringens isolates, i.e., 81% (9/11) of diarrheic piglets and 100% (18/18) of healthy piglets, and all of the genes had the same sequence. In conclusion, the β2 toxin gene of C. perfringens was distributed widely in Korean piglets regardless of the incidence of diarrhea, and there was no clear relationship with enteric disease. A pulsed-field gel electrophoresis analysis of DNA digested using SmaI demonstrated the non-clonal spread of C. perfringens isolates from piglets.
authors.affiliation: Viral Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do 430-824, South Korea.
authors.forename: Ki-Eun
authors.initials: KE
authors.lastname: Lee
authors.forename: Seong-In
authors.initials: SI
authors.lastname: Lim
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Shin
authors.forename: Yong-Kuk
authors.initials: YK
authors.lastname: Kwon
authors.forename: Ha-Young
authors.initials: HY
authors.lastname: Kim
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Song
authors.forename: Dong-Jun
authors.initials: DJ
authors.lastname: An
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics; D003015:Clostridium Infections / Q000453:epidemiology / Q000662:veterinary*; D003016:Clostridium perfringens / Q000235:genetics*; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D005243:Feces / Q000382:microbiology; D060885:Multiplex Polymerase Chain Reaction / Q000662:veterinary; D010802:Phylogeny; D056910:Republic of Korea / Q000453:epidemiology; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000382:microbiology*
chemical_list: D001427:Bacterial Toxins; D017931:DNA Primers; C109896:cpb2 protein, Clostridium perfringens