title: Quantification of protein concentration using UV absorbance and Coomassie dyes.
abstract: The measurement of a solubilized protein concentration in solution is an important assay in biochemistry research and development labs for applications ranging from enzymatic studies to providing data for biopharmaceutical lot release. Spectrophotometric protein quantification assays are methods that use UV and visible spectroscopy to rapidly determine the concentration of protein, relative to a standard, or using an assigned extinction coefficient. Where multiple samples need measurement, and/or the sample volume and concentration is limited, preparations of the Coomassie dye commonly known as the Bradford assay can be used.
authors.affiliation: Analytical Science, NPL, Teddington, Middlesex, UK. Electronic address: james.noble@npl.co.uk.
authors.forename: James E
authors.initials: JE
authors.lastname: Noble
mesh_terms: D005069:Evaluation Studies as Topic; D007202:Indicators and Reagents / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D012015:Reference Standards; D012394:Rosaniline Dyes / Q000737:chemistry*; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet / Q000592:standards; D013194:Staining and Labeling
chemical_list: D007202:Indicators and Reagents; D011506:Proteins; D012394:Rosaniline Dyes; D012996:Solutions; C048139:Coomassie blue
keywords: Beer-Lambert law; Bradford assay; Coomassie dyes; Protein concentration; UV absorbance