title: Preparation and evaluation of collagen-silk fibroin/hydroxyapatite nanocomposites for bone tissue engineering.
abstract: A new in situ precipitation technique was developed to synthesize collagen-silk fibroin/hydroxyapatite nanocomposites. The componential properties and morphological of nanocomposites were investigated. It was revealed that the inorganic phase in the nanocomposite was carbonate-substituted hydroxyapatite with low crystallinity. Morphology studies showed that hydroxyapatite particles with size ranging from 30 to 100 nm were distributed uniformly in the polymer matrix. According to the TEM micrographs, inorganic particles were composed of more fine sub-particles whose diameters were between 2 and 5 nm in size without regular crystallographic orientation. The mechanical properties of the composites were evaluated by measuring their elastic modulus. The data indicated that the elastic modulus of nanocomposites was improved by the addition of silk fibroin. Finally, the cell biocompatibility of the composites was tested in vitro, which showed that they have good biocompatibility. These results suggest that the collagen-silk fibroin/hydroxyapatite nanocomposites are promising biomaterials for bone tissue engineering.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Jingxiao
authors.initials: J
authors.lastname: Hu
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Jiabing
authors.initials: J
authors.lastname: Ran
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China. Electronic address: shenxy@whu.edu.cn.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Shen
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Tong
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D001842:Bone and Bones / Q000166:cytology* / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000378:metabolism*; D017886:Durapatite / Q000378:metabolism*; D005349:Fibroins / Q000378:metabolism*; D055595:Mechanical Phenomena; D053761:Nanocomposites; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D003094:Collagen; D005349:Fibroins; D017886:Durapatite
keywords: Collagen; Hydroxyapatite; Silk fibroin