title: In vivo [35S]-methionine incorporation.
abstract: The purpose of this assay is to measure the incorporation of radiolabeled [(35)S]-methionine into newly synthesized proteins in exponentially growing yeast cells. This allows for a quantitative in vivo measurement of total protein synthesis.
authors.affiliation: UMDNJ Robert Wood Johnson Medical School, Piscataway, NJ, USA.
authors.forename: Anthony M
authors.initials: AM
authors.lastname: Esposito
authors.affiliation: UMDNJ Robert Wood Johnson Medical School, Piscataway, NJ, USA. Electronic address: kinzytg@umdnj.edu.
authors.forename: Terri Goss
authors.initials: TG
authors.lastname: Kinzy
mesh_terms: D011232:Chemical Precipitation; D003470:Culture Media; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism; D007553:Isotope Labeling; D008715:Methionine / Q000378:metabolism*; D013462:Sulfur Radioisotopes / Q000378:metabolism; D015003:Yeasts / Q000378:metabolism
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D013462:Sulfur Radioisotopes; D008715:Methionine
keywords: Bacterial and viral pathogens; In vivo [(35)S]-methionine; Lyse cells and TCA precipitate proteins; Preparation of culture; Protein synthesis; Scintillation counter and analyze data