title: Identifications of household's spores using mid infrared spectroscopy.
abstract: Exposure to household fungi is very common both inside and outside the house and can cause health issues. The application of fourier transforms mid infrared spectroscopy (FTIR) as a screening technique for the detection and identification of household fungi was investigated. Early detection and identification of these household pathogens is very important and critical for their control. The current available methods for identification of fungi are time consuming, expensive and not very specific. Mid IR spectroscopy is a reliable and sensitive technique for the detection of spores. FTIR Spectra of four household fungi such as Aspergillus Ochraceus, Aspergillus Niger, Candida Glabrata and Penicillium Roguefortii were recorded in the mid infrared range from 600 to 4000cm(-1) using attenuated total reflectance (ATR) sampling accessory. Chemometrics analysis using principal component analysis (PCA), canonical variate analysis (CVA) and linear discriminant analysis (LDA) were performed to discriminate the fungi samples. Correspondence analysis (CA) was performed in order to visualize the relationship between different spores. An optimum classification of 100% was achieved for four different fungi. Results demonstrated that discriminant analysis of the FTIR spectra of fungi could be used for rapid detection of household fungi.
authors.affiliation: Fiber Science, Cornell University, Ithaca, NY 14251, USA.
authors.forename: Vivechana
authors.initials: V
authors.lastname: Dixit
authors.affiliation: College of Agriculture and Life Science, Chungnam National University, Yusung-Gu, Daejeon 305-764, Republic of Korea.
authors.forename: Byoung Kwan
authors.initials: BK
authors.lastname: Cho
authors.affiliation: Fiber Science, Cornell University, Ithaca, NY 14251, USA.
authors.forename: Kay
authors.initials: K
authors.lastname: Obendorf
authors.affiliation: Fiber Science, Cornell University, Ithaca, NY 14251, USA. Electronic address: jctewari@yahoo.com.
authors.forename: Jagdish
authors.initials: J
authors.lastname: Tewari
mesh_terms: D001230:Aspergillus / Q000737:chemistry / Q000302:isolation & purification; D002175:Candida / Q000737:chemistry / Q000302:isolation & purification; D016002:Discriminant Analysis; D004783:Environmental Microbiology; D005658:Fungi / Q000737:chemistry* / Q000302:isolation & purification; D010407:Penicillium / Q000737:chemistry / Q000302:isolation & purification; D025341:Principal Component Analysis; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013172:Spores, Fungal / Q000737:chemistry* / Q000302:isolation & purification
keywords: Chemometrics; FTIR-ATR; PCA and CA