title: Assembly of chemically modified G-rich sequences into tetramolecular DNA G-quadruplexes and higher order structures.
abstract: In this review, we introduce the biophysical and biochemical methods currently used to investigate the structures and stabilities of tetramolecular DNA G-quadruplexes containing chemical modifications. We hope this paper will guide others as they perform similar experiments leading to more information about the effects of chemical modifications on G-quadruplex formation. The structures of tetramolecular quadruplexes and some higher order structures based on tetramolecular quadruplexes are also described.
authors.affiliation: Univ. Bordeaux, ARNA Laboratory, F-33000 Bordeaux, France; INSERM, U869, IECB, F-33600 Pessac, France.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.affiliation: INSERM, US001, CNRS UMS 3033, Univ. Bordeaux, IECB, F-33600 Pessac, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Rosu
authors.affiliation: Univ. Bordeaux, ARNA Laboratory, F-33000 Bordeaux, France; INSERM, U869, IECB, F-33600 Pessac, France.
authors.forename: Samir
authors.initials: S
authors.lastname: Amrane
authors.affiliation: Univ. Bordeaux, ARNA Laboratory, F-33000 Bordeaux, France; INSERM, U869, IECB, F-33600 Pessac, France.
authors.forename: Dursun Nizam
authors.initials: DN
authors.lastname: Korkut
authors.affiliation: Univ. Bordeaux, ARNA Laboratory, F-33000 Bordeaux, France; INSERM, U869, IECB, F-33600 Pessac, France. Electronic address: v.gabelica@iecb.u-bordeaux.fr.
authors.forename: Valérie
authors.initials: V
authors.lastname: Gabelica
authors.affiliation: Univ. Bordeaux, ARNA Laboratory, F-33000 Bordeaux, France; INSERM, U869, IECB, F-33600 Pessac, France. Electronic address: jean-louis.mergny@inserm.fr.
authors.forename: Jean Louis
authors.initials: JL
authors.lastname: Mergny
mesh_terms: D001482:Base Composition; D020029:Base Pairing; D001483:Base Sequence; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D054856:G-Quadruplexes; D006801:Humans; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D044366:Transition Temperature
chemical_list: D004247:DNA
keywords: CD; Chemical modification; G-quadruplex; Mass spectrometry; NMR; UV