title: Purification, crystallization and preliminary X-ray crystallographic analysis of a rice Rac/Rop GTPase, OsRac1.
abstract: Small GTPases regulate a large variety of key cellular processes. Plant small Rac/Rop GTPases have recently received broad attention as it is becoming clear that these enzymes regulate various plant cellular processes. OsRac1, a rice Rac/Rop protein, is a key regulator of reactive oxygen species (ROS) production and induces immune responses. Although four structures of plant small GTPases have been reported, all of these were of the inactive form. Here, OsRac1 was purified and co-crystallized with the GTP analogue 5'-guanylyl imidodiphosphate (GMPPNP). The crystal belonged to space group P2(1)2(1)2(1) and a complete data set was collected to 1.9 Å resolution.
authors.affiliation: Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Kosami
authors.affiliation: Laboratory of Biophysics, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
authors.forename: Izuru
authors.initials: I
authors.lastname: Ohki
authors.affiliation: Laboratory of Biophysics, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
authors.forename: Kokoro
authors.initials: K
authors.lastname: Hayashi
authors.affiliation: Laboratory of Biophysics, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Tabata
authors.affiliation: Laboratory of Biophysics, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
authors.forename: Sayaka
authors.initials: S
authors.lastname: Usugi
authors.affiliation: Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Kawasaki
authors.affiliation: Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Toshimichi
authors.initials: T
authors.lastname: Fujiwara
authors.affiliation: Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nakagawa
authors.affiliation: Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
authors.forename: Ko
authors.initials: K
authors.lastname: Shimamoto
authors.affiliation: Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Chojiro
authors.initials: C
authors.lastname: Kojima
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D006165:Guanylyl Imidodiphosphate / Q000737:chemistry; D012275:Oryza / Q000201:enzymology*; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D020744:rac GTP-Binding Proteins / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D010940:Plant Proteins; D006165:Guanylyl Imidodiphosphate; D020744:rac GTP-Binding Proteins
keywords: GTP-binding form; OsRac1; small GTPase