title: [Effect of modified sijunzi decoction on the bone metabolism of adriamycin induced nephropathy rats].
abstract: OBJECTIVE
  To explore the effect of Modified Sijunzi Decoction (MSD) on the bone metabolism of prednisone intervened adriamycin-induced nephropathy rats.
  
  
  METHODS
  The adriamycin-induced nephropathy rat model was prepared. Totally 50 SD rats were randomly divide into five groups, i.e., the model group, the hormone group, the Chinese medicine (CM) group, the CM + hormone group, and the normal control group. The 24-h urine samples were collected on the 7th, 21st, and 35th day after modeling. The 24-h urine protein was measured by biuret colorimetry. Serum levels of osteoprotegerin (OPG), receptor activator of nuclear factor-kappaB ligand (RANKL), osteocalcin (BGP), and tartrate-resistant acid phosphatase (TRACP) were determined by ELISA. Expressions of OPG and RANKL in the tibia tissue were detected using real-time quantitative PCR and Western blot.
  
  
  RESULTS
  (1) Compared with the normal control group, the 24-h urine protein increased in each group on the 7th, 21st, and 35th day (P < 0.05, P < 0.01). Compared with the model group, the 24-h urinary protein decreased in the hormone group and the CM + hormone group (P < 0.05, P < 0.01). The decrement was more obvious along with the treatment time went by (P < 0.05, P < 0.01). There was statistical difference in the reduction of urine protein on the 35th day between the CM group and the model group (P < 0.05). (2) Compared with the 21st-day of the same group, the serum levels of TRACP and RANKL increased (P < 0.05, P < 0.01). Compared with the model group, the serum levels of the TRACP and RANKL increased (P < 0.05, P < 0.01), OPG and BGP decreased (P < 0.05, P < 0.01) in the hormone group. Compared with the CM group at the same period, serum OPG level decreased and the RANKL level increased in the hormone group and the CM + hormone group (P < 0.05, P < 0.01). Besides, the serum level of TRACP increased and BGP decreased (P < 0.05, P < 0.01). Compared with the hormone group at the same period, OPG and BGP increased (P < 0.05, P < 0.01), RANKL decreased (P < 0.01) in the CM + hormone group. On the 35th day TRACP decreased (P < 0.01). (3) Compared with the normal group, mRNA expressions of OPG and RANKL on the 21st day increased (P < 0.05, P < 0.01), mRNA expressions of OPG and RANKL on the 35th day decreased in the model group (P < 0.01). Compared with the CM group at the same period, OPG mRNA expression decreased (P < 0.01) and RANKL mRNA expression increased in the hormone group (P < 0.05). OPG mRNA expression decreased in the CM +hormone group (P < 0.05). (4) Compared with the hormone group on the 21st day, the OPG level decreased and the RANKL protein increased (both P < 0.05). RANKL decreased in the CM + hormone group (P < 0.05). Compared with the model group at the same period, OPG decreased and RANKL increased in the hormone group (P < 0.01). Compared with the CM group at the same period, OPG decreased (P < 0.01), RANKL increased (P < 0.01) in the hormone group and the CM + hormone group. Compared with the hormone group at the same period, OPG increased and RANKL decreased in the CM + hormone group (both P < 0.01).
  
  
  CONCLUSIONS
  Prednisone could induce osteoporosis through the OPG/RANKL/RANK pathway. MSZ could slow down the formation of prednisone-induced osteoporosis through promoting osteoblast differentiation, and inhibiting osteoclastogenesis.
authors.affiliation: Department of Nephropathy, Fujian Provincial People's Hospital, Fujian University of Traditional Chinese Medicine, Fuzhou 350004, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Department of Nephropathy, Guizhou Provincial People' s Hospital, Guiyang 550000, China.
authors.forename: Jia-Lin
authors.initials: JL
authors.lastname: Liu
authors.affiliation: Department of Nephropathy, Fujian Provincial People's Hospital, Fujian University of Traditional Chinese Medicine, Fuzhou 350004, China.
authors.forename: Min-Fang
authors.initials: MF
authors.lastname: Lin
authors.affiliation: Department of Nephropathy, Fujian Provincial People's Hospital, Fujian University of Traditional Chinese Medicine, Fuzhou 350004, China.
authors.forename: Zhen-Fei
authors.initials: ZF
authors.lastname: Wang
authors.affiliation: Fujian University of Traditional Chinese Medicine, Fuzhou 350100, China.
authors.forename: Ci-Yun
authors.initials: CY
authors.lastname: Liu
authors.affiliation: Fujian University of Traditional Chinese Medicine, Fuzhou 350100, China.
authors.forename: Xin-Hong
authors.initials: XH
authors.lastname: Wu
authors.affiliation: Department of Nephropathy, Fujian Provincial People's Hospital, Fujian University of Traditional Chinese Medicine, Fuzhou 350004, China.
authors.forename: Hua-Yang
authors.initials: HY
authors.lastname: Lin
authors.affiliation: Fujian University of Traditional Chinese Medicine, Fuzhou 350100, China.
authors.forename: Cai-Feng
authors.initials: CF
authors.lastname: Chen
authors.affiliation: Fujian University of Traditional Chinese Medicine, Fuzhou 350100, China.
authors.forename: Xue-Min
authors.initials: XM
authors.lastname: Zheng
authors.affiliation: Fujian University of Traditional Chinese Medicine, Fuzhou 350100, China.
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Chen
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D004317:Doxorubicin / Q000009:adverse effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D009401:Nephrosis / Q000139:chemically induced / Q000378:metabolism*; D015675:Osteocalcin / Q000378:metabolism; D053244:Osteoprotegerin / Q000378:metabolism; D011241:Prednisone / Q000494:pharmacology; D053245:RANK Ligand / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D000071681:Tartrate-Resistant Acid Phosphatase; D013977:Tibia / Q000378:metabolism*
chemical_list: D004365:Drugs, Chinese Herbal; D007527:Isoenzymes; D053244:Osteoprotegerin; D053245:RANK Ligand; C506032:Tnfrsf11b protein, rat; D015675:Osteocalcin; D004317:Doxorubicin; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D011241:Prednisone