title: Steady increment of immature platelet fraction is suppressed by irradiation in single-donor platelet components during storage.
abstract: Circulating immature platelet fraction (IPF) reflects real-time thrombopoiesis and correlates with platelet recovery from thrombocytopenic presentations. To understand the dynamics of IPF in platelet transfusions, we quantified the %-IPF in single-donor platelet components (SDP) during prolonged storage. %-IPF significantly increased from baseline by day 5 post-donation. Absolute IPF counts (A-IPC) had similar significant increments. However, gamma-irradiation suppressed the increments of %-IPF and A-IPC by >50%. Ultrastructural analysis of SDP units at day 10 showed well preserved morphology of immature platelets. Our findings suggest that IPF might actively expand ex-vivo and may have a longer shelf life than their mature counterparts. Closer study of IPF may be of critical clinical importance for transfusion practices.
authors.affiliation: Department of Pathology, University Hospitals Case Medical Center, Cleveland, Ohio, United States of America ; Case Western Reserve University School of Medicine, Cleveland, Ohio, United States of America.
authors.forename: Hong
authors.initials: H
authors.lastname: Hong
authors.affiliation: Department of Pathology, University Hospitals Case Medical Center, Cleveland, Ohio, United States of America ; Case Western Reserve University School of Medicine, Cleveland, Ohio, United States of America.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Xiao
authors.affiliation: Department of Pathology, University Hospitals Case Medical Center, Cleveland, Ohio, United States of America ; Case Western Reserve University School of Medicine, Cleveland, Ohio, United States of America.
authors.forename: Robert W
authors.initials: RW
authors.lastname: Maitta
mesh_terms: D001792:Blood Platelets / Q000528:radiation effects* / Q000648:ultrastructure; D049109:Cell Proliferation / Q000528:radiation effects*; D002478:Cells, Cultured; D005720:Gamma Rays; D006801:Humans; D046529:Microscopy, Electron, Transmission; D010976:Platelet Count; D017713:Platelet Transfusion; D013696:Temperature; D013921:Thrombocytopenia / Q000473:pathology / Q000628:therapy; D034061:Thrombopoiesis / Q000502:physiology; D013997:Time Factors