title: Chloramphenicol effects on chlorophyll degradation and photosystem I assembly in the chlorina CD3 wheat mutant.
abstract: We previously reported that applications of chloramphenicol to the chlorina wheat mutant, CD3, decreased the leaf Chl a/b ratio and enhanced accumulations of LHC proteins and LHC complexes during greening (Duysen et al. 1985). We have now examined Chl degradation and the change in Chl a/b ratios in wheat leaves kept in the dark as a measure of LHC destruction. Chl b was stable in chloroplasts of the CD3 wheat kept in darkness up to 5 days. Chloramphenicol significantly increased Chl b accumulations and impaired Chl a degradation in both CD3 mutant and normal wheat relative to untreated plants. Our Chl data suggest that the chloramphenicol induced accumulation of the LHC complex in the mutant wheat results from enhanced processing of LHC into the membrane rather than impairment of LHC degradation. The photosystem I (PSI) fraction of the CD3 wheat mutant was examined relative to that of normal wheat after 3 days greening. PSI was deficient in 25, 26, 26.5 kD LHCI protein in the mutant but both wheats accumulated low quantities of the 27-29 kD LHCII protein as detected by Western blot analysis. Chloramphenicol enhanced accumulations of several LHCI proteins primarily near 25 kD in the mutant and the 27-29 kD LHCII protein in normal wheat. The fluorescence emission and absorbance spectra suggest that chloramphenicol enhances accumulations of dissociated LHC in the PSI preparation of normal and CD3 mutant wheat.
authors.affiliation: Botany Department, North Dakota State University, 58105-5517, Fargo, North Dakota, USA.
authors.forename: M
authors.initials: M
authors.lastname: Duysen
authors.forename: L
authors.initials: L
authors.lastname: Huckle
authors.forename: K
authors.initials: K
authors.lastname: Mogen
authors.forename: T
authors.initials: T
authors.lastname: Freeman