title: Polycomb group oncogene RING1 is over-expressed in non-small cell lung cancer.
abstract: Ring finger protein 1 (RING1) have recently been reported to be related to aggressive tumor features in Prostate Cancer and urothelial carcinoma of the bladder. However, the role of RING1 in non-small-cell lung cancer (NSCLC) tumorigenesis has never been elucidated. So we aimed at investigating the potential role of RING1 in NSCLC. RING1 expression was evaluated by Immunoblot in 8 paired fresh lung cancer tissues and immunohistochemistry on 69 paraffin-embedded sections from 2006 to 2009. Furthermore, flow-cytometry and RNA interference were performed to analyse the role of RING1 in A549 cells. We showed that the expression level of RING1 was significant increased in lung cancer as compared with the adjacent normal tissue. High expression level of RING1 was associated with TNM stage (P = 0.013), and RING1 was positively related with proliferation marker Ki67 (P < 0.05). Moreover, RING1 knockdown induces growth suppression of human lung cancer cells through G1/S cell cycle phase arrest in vitro. Kaplan-Meier survival curves showed that high expression level of RING1 was associated with poor prognosis (P = 0.03). On the basis of these results, we suggested that RING1 protein expression may be a favorable independent prognostic parameter for non-small cell lung cancer.
authors.affiliation: Department of Thoracic Surgery, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Yiqun
authors.initials: Y
authors.lastname: Zhou
authors.forename: Chunhua
authors.initials: C
authors.lastname: Wan
authors.forename: Yifei
authors.initials: Y
authors.lastname: Liu
authors.forename: Liting
authors.initials: L
authors.lastname: Lv
authors.forename: Buyou
authors.initials: B
authors.lastname: Chen
authors.forename: Runzhou
authors.initials: R
authors.lastname: Ni
authors.forename: Yuexia
authors.initials: Y
authors.lastname: Huang
authors.forename: Yangcheng
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zheng
authors.forename: Dunpeng
authors.initials: D
authors.lastname: Yang
authors.forename: Guoxin
authors.initials: G
authors.lastname: Mao
authors.forename: Qun
authors.initials: Q
authors.lastname: Xue
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D018287:Carcinoma, Large Cell / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D002453:Cell Cycle; D049109:Cell Proliferation; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D008175:Lung Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D063150:Polycomb Repressive Complex 1 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis; D034741:RNA, Small Interfering / Q000235:genetics; D015996:Survival Rate; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D034741:RNA, Small Interfering; D063150:Polycomb Repressive Complex 1; C080355:RING1 protein, human