title: Transgenics in groundnut (Arachis hypogaea L.) expressing cry1AcF gene for resistance to Spodoptera litura (F.).
abstract: Large number of primary transgenic events were generated in groundnut by an Agrobacterium mediated, in planta transformation method to assess the efficacy of cry1AcF against the Spodoptera litura. The amplification of required size fragment of 750 bp with npt II primers and 901 bp with cry1AcF gene primers confirmed the integration of the gene. The expression of the cry gene was ascertained by ELISA in T2 generation, and the maximum concentration of cry protein in transgenic plants reached approximately 0.82 μg/g FW. Further, Southern blot analysis of ten T2 transgenic plants proved that transgene had been integrated in the genome of all the plants and Northern analysis of the same plants demonstrated the active expression of cry1AcF gene. The highest mean % larval mortalities 80.0 and 85.0 with an average mean % larval mortalities 16.25 (n = 369) and 26.0 (n = 80) were recorded in T1 and T2 generations, respectively. Segregation analysis of the selected lines in the T3 generation demonstrated homozygous nature. This clearly proved that though there is considerable improvement in average mean % larval mortality in T2 generation, the cry1AcF gene was effective against S. litura only to some extent.
authors.affiliation: Department of Agricultural Entomology, University of Agricultural Sciences, GKVK, Bangalore, 560065 India.
authors.forename: G
authors.initials: G
authors.lastname: Keshavareddy
authors.affiliation: Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bangalore, 560065 India.
authors.forename: S
authors.initials: S
authors.lastname: Rohini
authors.affiliation: Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bangalore, 560065 India.
authors.forename: S V
authors.initials: SV
authors.lastname: Ramu
authors.affiliation: Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bangalore, 560065 India.
authors.forename: S
authors.initials: S
authors.lastname: Sundaresha
authors.affiliation: Department of Agricultural Entomology, University of Agricultural Sciences, GKVK, Bangalore, 560065 India.
authors.forename: A R V
authors.initials: AR
authors.lastname: Kumar
authors.affiliation: National Research Centre on Plant Biotechnology, New Delhi, India.
authors.forename: P Ananda
authors.initials: PA
authors.lastname: Kumar
authors.affiliation: Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bangalore, 560065 India.
authors.forename: M
authors.initials: M
authors.lastname: Udayakumar
keywords: Groundnut; In planta transformation; Spodoptera litura; cry1AcF