title: Impaired GABAB receptor signaling dramatically up-regulates Kiss1 expression selectively in nonhypothalamic brain regions of adult but not prepubertal mice.
abstract: Kisspeptin, encoded by Kiss1, stimulates reproduction and is synthesized in the hypothalamic anteroventral periventricular and arcuate nuclei. Kiss1 is also expressed at lower levels in the medial amygdala (MeA) and bed nucleus of the stria terminalis (BNST), but the regulation and function of Kiss1 there is poorly understood. γ-Aminobutyric acid (GABA) also regulates reproduction, and female GABAB1 receptor knockout (KO) mice have compromised fertility. However, the interaction between GABAB receptors and Kiss1 neurons is unknown. Here, using double-label in situ hybridization, we first demonstrated that a majority of hypothalamic Kiss1 neurons coexpress GABAB1 subunit, a finding also confirmed for most MeA Kiss1 neurons. Yet, despite known reproductive impairments in GABAB1KO mice, Kiss1 expression in the anteroventral periventricular and arcuate nuclei, assessed by both in situ hybridization and real-time PCR, was identical between adult wild-type and GABAB1KO mice. Surprisingly, however, Kiss1 levels in the BNST and MeA, as well as the lateral septum (a region normally lacking Kiss1 expression), were dramatically increased in both GABAB1KO males and females. The increased Kiss1 levels in extrahypothalamic regions were not caused by elevated sex steroids (which can increase Kiss1 expression), because circulating estradiol and testosterone were equivalent between genotypes. Interestingly, increased Kiss1 expression was not detected in the MeA or BNST in prepubertal KO mice of either sex, indicating that the enhancements in extrahypothalamic Kiss1 levels initiate during/after puberty. These findings suggest that GABAB signaling may normally directly or indirectly inhibit Kiss1 expression, particularly in the BNST and MeA, and highlight the importance of studying kisspeptin populations outside the hypothalamus.
authors.affiliation: Institute of Biology and Experimental Medicine-CONICET (N.P.D.G., P.V.L., C.L., V.A.L-L.), Buenos Aires, Argentina; Department of Reproductive Medicine (S.J.S., J.K., A.S.K.), University of California San Diego, La Jolla, California; Department of Biomedicine (B.B.), University of Basel, Basel, Switzerland; and Department of Physiology (C.L.), University of Buenos Aires, Buenos Aires, Argentina.
authors.forename: Noelia P
authors.initials: NP
authors.lastname: Di Giorgio
authors.forename: Sheila J
authors.initials: SJ
authors.lastname: Semaan
authors.forename: Joshua
authors.initials: J
authors.lastname: Kim
authors.forename: Paula V
authors.initials: PV
authors.lastname: López
authors.forename: Bernhard
authors.initials: B
authors.lastname: Bettler
authors.forename: Carlos
authors.initials: C
authors.lastname: Libertun
authors.forename: Victoria A
authors.initials: VA
authors.lastname: Lux-Lantos
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Kauffman
mesh_terms: D000679:Amygdala / Q000378:metabolism; D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000378:metabolism; D001931:Brain Mapping; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D005838:Genotype; D007031:Hypothalamus / Q000378:metabolism; D007150:Immunohistochemistry; D059648:Kisspeptins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D020644:Midline Thalamic Nuclei / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D010641:Phenotype; D018080:Receptors, GABA-B / Q000235:genetics / Q000378:metabolism*; D012686:Septal Nuclei / Q000378:metabolism; D015398:Signal Transduction; D013739:Testosterone / Q000378:metabolism; D013997:Time Factors; D015854:Up-Regulation; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: C491407:Kiss1 protein, mouse; D059648:Kisspeptins; D018080:Receptors, GABA-B; D013739:Testosterone; D004958:Estradiol; D005680:gamma-Aminobutyric Acid