title: Soluble complement complex C5b-9 promotes microglia activation.
abstract: Soluble C5b-9 has been described as a pro-inflammatory mediator that triggers cell activation rather than inducing cell death. Microglia is the most important immune cell involved in inflammatory response in the CNS. Although microglia activation induced by various stimuli has been well characterized, the role of C5b-9 in microglia has not been well studied. In the current experiment, we utilized assembled functional C5b-9 to treat microglia and analyzed the function. We found that soluble C5b-9 could promote microglia activation by up-regulation of costimulatory molecules and increase cytokine secretion. Our results suggested that soluble C5b-9 possessed immunoregulatory potential on microglia.
authors.affiliation: Department of Neurology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Cardiology, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Neurology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China. Electronic address: lzsht@aliyun.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D002478:Cells, Cultured; D015938:Complement Membrane Attack Complex / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology*; D020778:Matrix Metalloproteinase 2; D017628:Microglia / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D015398:Signal Transduction / Q000187:drug effects; D013702:Temporal Lobe / Q000166:cytology; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D015938:Complement Membrane Attack Complex; D007155:Immunologic Factors; C047610:SC5b-9 protein complex; D020778:Matrix Metalloproteinase 2
keywords: Activation; C5b-9; Microglia