title: Immunization with outer-membrane protein(s) of Bacteroides fragilis: an experimental study in mice.
abstract: A crude outer-membrane protein (OMP) preparation from a strain of Bacteroides fragilis, grown in supplemented brain-heart infusion broth, was tested for its protective effect against subcutaneous infection in mice. Immunization with six doses, each of 100, 150 or 200 μg OMP preparation, gave some protection: abscesses completely disappeared 15 to 22 days after immunization. In non-immunized animals and animals immunized with doses of 10, 20, 40 or 80 μg each, well demarcated abscesses were seen beyond day 22 post-immunization. Although crude OMP elicited good antibody response, with maximum titres on day 4 post-immunization, high titres could not be associated with healing of the abscesses.
authors.affiliation: Department of Medical Microbiology, Postgraduate Medical Institute, Chandigarh, India.
authors.forename: T
authors.initials: T
authors.lastname: Khanna
authors.forename: D
authors.initials: D
authors.lastname: Panigrahi
authors.forename: N K
authors.initials: NK
authors.lastname: Ganguly
authors.forename: S
authors.initials: S
authors.lastname: Majumdar