title: The intracellular location of the enzymes of nitrate assimilation in the apices of seedling pea roots.
abstract: The intracellular distribution of the enzymes of nitrate and ammonia assimilation in apical cells of pea (Pisum sativum L.) roots is described. Nitrate reductase (EC 1.6.6.2) was found to have no organelle association, and is considered to be located in the cytosol or possibly loosely bound to the outside of an organelle. Nitrite reductase and glutamate synthase (EC 2.6.1.53) are plastid located, as is glutamine synthetase (EC 6.3.1.2) although this enzyme also has activity in the cytosol. Glutamate dehydrogenase (EC 1.4.1.3) was found only in the mitochondrion.
authors.affiliation: Department of Biochemistry, The University, S10 2TN, Sheffield, UK.
authors.forename: M J
authors.initials: MJ
authors.lastname: Emes
authors.forename: M W
authors.initials: MW
authors.lastname: Fowler