title: Upregulated TIMP-1 correlates with poor prognosis of laryngeal squamous cell carcinoma.
abstract: The tissue inhibitor of metalloproteinase-1 (TIMP-1) is an endogenous inhibitor of matrix metalloproteinases and potential biomarker of various types of human cancers. However, the association between TIMP-1 expression and the clinical features of laryngeal squamous cell carcinoma (LSCC) is barely investigated. In this study, one-step quantitative reverse transcription-polymerase chain reaction and immunohistochemical staining with tissue microarrays were employed to evaluate the relationship between TIMP-1 expression and the clinicopathological characteristics of LSCC. Results showed that the TIMP-1 mRNA and protein expression levels were significantly higher in LSCC than in the corresponding non-cancerous tissues (p<0.05). TIMP-1 protein expression in LSCC was associated with tumor differentiation (p=0.012) and overall survival (p=0.043). Kaplan-Meier method and Cox multi-factor analysis suggested that high TIMP-1 expression (p=0.008) and positive lymph node metastasis (p=0.029) were significantly associated with the poor survival of patients with LSCC. These data indicated that TIMP-1 may be identified as a prognostic marker of LSCC.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Yiji Shan Hospital, The First Affiliated Hospital of Wannan Medical College No. 92 Zheshan Xi Road, Wuhu 241000, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Hematology and Oncology, Jiangsu Province Geriatric Institute No. 30 Luojia Road, Nanjing 210029, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Hematology and Oncology, Jiangsu Province Geriatric Institute No. 30 Luojia Road, Nanjing 210029, China.
authors.forename: Weifei
authors.initials: W
authors.lastname: Fan
authors.affiliation: Department of Hematology and Oncology, Jiangsu Province Geriatric Institute No. 30 Luojia Road, Nanjing 210029, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Pu
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated Hospital of Nanjing Medical University No. 121 Jiang Jia Yuan, Nanjing 210011, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Yiji Shan Hospital, The First Affiliated Hospital of Wannan Medical College No. 92 Zheshan Xi Road, Wuhu 241000, China.
authors.forename: Chou
authors.initials: C
authors.lastname: Fan
authors.affiliation: Department of Pathology, The Second Affiliated Hospital of Nanjing Medical University No. 121 Jiang Jia Yuan, Nanjing 210011, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xiong
authors.affiliation: Department of Pathology and Laboratory Medicine, Nantong University Affiliated Hospital No. 20 Xisi Road, Nantong 226001, China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Pathology, Nanjing Medical University No. 140 Hanzhong Road, Nanjing 210029, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Xu
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated Hospital of Nanjing Medical University No. 121 Jiang Jia Yuan, Nanjing 210011, China.
authors.forename: Renjie
authors.initials: R
authors.lastname: Chen
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Yiji Shan Hospital, The First Affiliated Hospital of Wannan Medical College No. 92 Zheshan Xi Road, Wuhu 241000, China.
authors.forename: Shaofeng
authors.initials: S
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D008207:Lymphatic Metastasis / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D016016:Proportional Hazards Models; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046888:Tissue Array Analysis; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000032:analysis / Q000096:biosynthesis*; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C560822:TIMP1 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1
keywords: LSCC; TIMP-1; immunohistochemistry; prognosis; qPCR