title: Mucilaginibacter polytrichastri sp. nov., isolated from a moss (Polytrichastrum formosum), and emended description of the genus Mucilaginibacter.
abstract: A Gram-staining-negative, rod-shaped, non-spore-forming bacterium, designated strain RG4-7(T), was isolated from the moss Polytrichastrum formosum collected from Gawalong glacier in Tibet, China, and characterized by using a polyphasic taxonomic approach. The predominant fatty acids of strain RG4-7(T) were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH. The major polar lipids were phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified phospholipid, two unidentified aminolipids and one unidentified lipid. Strain RG4-7(T) contained MK-7 as the dominant menaquinone and the G+C content of its genomic DNA was 39.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain RG4-7(T) was affiliated to species of the genus Mucilaginibacter, and its closest relative was Mucilaginibacter jinjuensis YC7004(T) (97.0 % sequence similarity). However, the DNA-DNA relatedness between this strain and strain RG4-7(T) was only 49.1±3.7 %. Based on phylogenetic inference and phenotypic data, strain RG4-7(T) is considered to represent a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter polytrichastri sp. nov. is proposed. The type strain is RG4-7(T) ( = CGMCC 1.12493(T) = DSM 26907(T)). An emended description of the genus Mucilaginibacter is also proposed.
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Xin Yao
authors.initials: XY
authors.lastname: Chen
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Ran
authors.initials: R
authors.lastname: Zhao
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Tian
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Bi He
authors.initials: BH
authors.lastname: Kong
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Xue Dong
authors.initials: XD
authors.lastname: Li
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Zhi Ling
authors.initials: ZL
authors.lastname: Chen
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Yan Hong
authors.initials: YH
authors.lastname: Li
mesh_terms: D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D044002:Bryophyta / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D046448:Ice Cover; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D018609:Tibet; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7