title: Scedosporium prolificans immunomes against human salivary immunoglobulin A.
abstract: The filamentous fungus Scedosporium prolificans is an emerging multidrug resistant pathogen related to serious infections mainly affecting immunocompromised individuals. Considering that it is frequently isolated from anthropic environments and penetrates mainly through the airways, the human mucosal immune system may play an important protective role against S. prolificans. To advance in the search for biomarkers and targets both for diagnosis and treatment, we analysed the S. prolificans immunomes recognized by human salivary Immunoglobulin A. Using indirect immunofluorescence, it was observed that conidia were strongly recognized, while hyphae were not. By 2-D immunoblotting and peptide mass fingerprinting, 25 immunodominant antigens in conidia and 30 in hyphae were identified. These included catalase, putative glyceronetransferase, translation elongation factor-1α, serine/threonine protein kinase, putative superoxide dismutase, putative mitochondrial cyclophilin 1 and peptidyl-prolyl cis-trans isomerase in conidiospores, and putative Hsp60, ATP synthase β chain, 40S ribosomal protein S0, citrate synthase and putative ATP synthase in hyphae. The functional study showed that metabolism - and protein fate - related enzymes were the most abundant antigens in conidia, whereas metabolism - , translation - , or energy production - related enzymes were in hyphae. The immunogenic proteins identified are proposed as candidates for the development of novel diagnostic tools or therapeutic strategies.
authors.affiliation: Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Leioa 48940, Bizkaia, Spain. Electronic address: aize.pellon@ehu.es.
authors.forename: Aize
authors.initials: A
authors.lastname: Pellon
authors.affiliation: Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Leioa 48940, Bizkaia, Spain; Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz 01006, Spain.
authors.forename: Andoni
authors.initials: A
authors.lastname: Ramirez-Garcia
authors.affiliation: Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Leioa 48940, Bizkaia, Spain.
authors.forename: Aitziber
authors.initials: A
authors.lastname: Antoran
authors.affiliation: Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Leioa 48940, Bizkaia, Spain.
authors.forename: Jimena Victoria
authors.initials: JV
authors.lastname: Fernandez-Molina
authors.affiliation: Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Leioa 48940, Bizkaia, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Abad-Diaz-de-Cerio
authors.affiliation: Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Leioa 48940, Bizkaia, Spain.
authors.forename: Dalila
authors.initials: D
authors.lastname: Montañez
authors.affiliation: Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Leioa 48940, Bizkaia, Spain.
authors.forename: Maria Jesus
authors.initials: MJ
authors.lastname: Sevilla
authors.affiliation: Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Leioa 48940, Bizkaia, Spain.
authors.forename: Aitor
authors.initials: A
authors.lastname: Rementeria
authors.affiliation: Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Leioa 48940, Bizkaia, Spain.
authors.forename: Fernando L
authors.initials: FL
authors.lastname: Hernando
mesh_terms: D000908:Antibodies, Fungal / Q000276:immunology*; D000946:Antigens, Fungal / Q000276:immunology*; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D025301:Hyphae / Q000276:immunology; D015151:Immunoblotting; D016056:Immunodominant Epitopes / Q000276:immunology; D007123:Immunoelectrophoresis, Two-Dimensional; D007070:Immunoglobulin A / Q000276:immunology*; D013058:Mass Spectrometry; D012463:Saliva / Q000276:immunology*; D021681:Scedosporium / Q000276:immunology*; D013172:Spores, Fungal / Q000276:immunology
chemical_list: D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D016056:Immunodominant Epitopes; D007070:Immunoglobulin A
keywords: Petriella; Two-dimensional electrophoresis; antigen; immunoblot; proteome