title: Effect of ATP-binding cassette subfamily B member 1 on bovine blastocyst implantation.
abstract: The ATP-binding cassette subfamily B member 1 (ABCB1) is an efflux transporter that excretes xenobiotics and waste matter. High expression of ABCB1 induced by forskolin (FSK) and rifampicin (RIF) in the bovine blastocysts reportedly improves the cellular quality. In the present study, interferon-α, similar to FSK and RIF, was highly potent in inducing the expression of ABCB1 in the bovine blastocysts but did not exhibit an additive effect with FSK and RIF. Bovine blastocysts stimulated by the combined treatment with FSK, RIF, and interferon-α to express high levels of ABCB1 displayed better freezing resistance as indicated by higher cell numbers in post thawing cultures. On transfer to recipients, such embryos established pregnancies with significantly higher frequencies in repeat breeder cows rather than normal ones.
authors.affiliation: Department of Animal Production, Fukuoka Agricultural Research Center, Chikushino, Fukuoka, Japan; Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University, Fukuoka, Fukuoka, Japan. Electronic address: dcb000653151@sgr.bbiq.jp.
authors.forename: M
authors.initials: M
authors.lastname: Mori
authors.affiliation: Embryo Transfer Section, Veterinary Clinic Center of Nosai Chikugo River Regions, Asakura, Fukuoka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kuwano
authors.affiliation: Department of Animal Production, Fukuoka Agricultural Research Center, Chikushino, Fukuoka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kamori
authors.affiliation: Department of Animal Production, Fukuoka Agricultural Research Center, Chikushino, Fukuoka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Isozaki
authors.affiliation: Embryo Transfer Section, Veterinary Clinic Center of Nosai Chikugo River Regions, Asakura, Fukuoka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nishihara
authors.affiliation: Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University, Fukuoka, Fukuoka, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Yamauchi
authors.affiliation: Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University, Fukuoka, Fukuoka, Japan.
authors.forename: M-A
authors.initials: MA
authors.lastname: Hattori
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D000818:Animals; D001755:Blastocyst / Q000737:chemistry / Q000166:cytology / Q000502:physiology; D002417:Cattle / Q000196:embryology*; D002452:Cell Count; D005576:Colforsin / Q000494:pharmacology; D015925:Cryopreservation / Q000662:veterinary; D004347:Drug Interactions; D010064:Embryo Implantation / Q000502:physiology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D016898:Interferon-alpha / Q000494:pharmacology; D011247:Pregnancy; D012293:Rifampin / Q000494:pharmacology
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D016898:Interferon-alpha; D005576:Colforsin; D012293:Rifampin
keywords: ABCB1; Bovine blastocyst; Cell proliferation; Conception; IVF