title: Podosomes of dendritic cells facilitate antigen sampling.
abstract: Dendritic cells sample the environment for antigens and play an important role in establishing the link between innate and acquired immunity. Dendritic cells contain mechanosensitive adhesive structures called podosomes that consist of an actin-rich core surrounded by integrins, adaptor proteins and actin network filaments. They facilitate cell migration via localized degradation of extracellular matrix. Here, we show that podosomes of human dendritic cells locate to spots of low physical resistance in the substrate (soft spots) where they can evolve into protrusive structures. Pathogen recognition receptors locate to these protrusive structures where they can trigger localized antigen uptake, processing and presentation to activate T-cells. Our data demonstrate a novel role in antigen sampling for the podosomes of dendritic cells.
authors.affiliation: Department of Tumor Immunology Radboud University Medical Centre Radboud Centre for Molecular Life Sciences Geert Grooteplein 28 6525GA Nijmegen The Netherlands.
authors.forename: Maksim
authors.initials: M
authors.lastname: Baranov
authors.affiliation: Department of Tumor Immunology Radboud University Medical Centre Radboud Centre for Molecular Life Sciences Geert Grooteplein 28 6525GA Nijmegen The Netherlands.
authors.forename: Martin
authors.initials: M
authors.lastname: Ter Beest
authors.affiliation: Department of Tumor Immunology Radboud University Medical Centre Radboud Centre for Molecular Life Sciences Geert Grooteplein 28 6525GA Nijmegen The Netherlands.
authors.forename: Inge
authors.initials: I
authors.lastname: Reinieren-Beeren
authors.affiliation: Department of Tumor Immunology Radboud University Medical Centre Radboud Centre for Molecular Life Sciences Geert Grooteplein 28 6525GA Nijmegen The Netherlands.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Cambi
authors.affiliation: Department of Tumor Immunology Radboud University Medical Centre Radboud Centre for Molecular Life Sciences Geert Grooteplein 28 6525GA Nijmegen The Netherlands.
authors.forename: Carl G
authors.initials: CG
authors.lastname: Figdor
authors.affiliation: Department of Tumor Immunology Radboud University Medical Centre Radboud Centre for Molecular Life Sciences Geert Grooteplein 28 6525GA Nijmegen The Netherlands.
authors.forename: Geert
authors.initials: G
authors.lastname: van den Bogaart
mesh_terms: D017951:Antigen Presentation; D022081:Cell Surface Extensions / Q000378:metabolism*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D004705:Endocytosis; D006801:Humans; D053511:Matrix Metalloproteinase 14 / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism
chemical_list: D014404:Tubulin; C507086:MMP14 protein, human; D053511:Matrix Metalloproteinase 14
keywords: Antigen presentation; Dendritic cell; Podosome; Receptor-mediated endocytosis