title: Overexpression of kallikrein gene 10 is a biomarker for predicting poor prognosis in gastric cancer.
abstract: OBJECTIVE
  To analyze the expression of kallikrein gene 10 (KLK10) in gastric cancer and to determine whether KLK10 has independent prognostic value in gastric cancer.
  
  
  METHODS
  We studied KLK10 expression in 80 histologically confirmed gastric cancer samples using real-time quantitative reverse transcription-PCR and hK10 expression using immunohistochemistry. Correlations with clinicopathological variables (lymph node metastasis, depth of invasion and histology) and with outcomes (disease-free survival and overall survival) during a median follow-up period of 31 mo were assessed. Gastric cancer tissues were then classified as KLK10 positive or negative.
  
  
  RESULTS
  KLK10 was found to be highly expressed in 57/80 (70%) of gastric cancer samples, while its expression was very low in normal gastric tissues. Positive relationships between KLK10 expression and lymph node metastasis (P = 0.048), depth of invasion (P = 0.034) and histology (P = 0.015) were observed. Univariate survival analysis revealed that gastric cancer patients with positive KLK10 expression had an increased risk for relapse/metastasis and death (P = 0.005 and 0.002, respectively). Cox multivariate analysis indicated that KLK10 was an independent prognostic indicator of disease-free survival and overall survival in patients with gastric cancer.
  
  
  CONCLUSIONS
  KLK10 expression is an independent biomarker of unfavorable prognosis in patients with gastric cancer.
authors.affiliation: Xin Jiao, Hai-Ning Zhi, Xiao-Man Liu, Chen-Hao Liu, Department of General Surgery, the First Central Clinical School, Tianjin Medical University, Tianjin 300192, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Jiao
authors.affiliation: Xin Jiao, Hai-Ning Zhi, Xiao-Man Liu, Chen-Hao Liu, Department of General Surgery, the First Central Clinical School, Tianjin Medical University, Tianjin 300192, China.
authors.forename: Hong-Jun
authors.initials: HJ
authors.lastname: Lu
authors.affiliation: Xin Jiao, Hai-Ning Zhi, Xiao-Man Liu, Chen-Hao Liu, Department of General Surgery, the First Central Clinical School, Tianjin Medical University, Tianjin 300192, China.
authors.forename: Mi-Mi
authors.initials: MM
authors.lastname: Zhai
authors.affiliation: Xin Jiao, Hai-Ning Zhi, Xiao-Man Liu, Chen-Hao Liu, Department of General Surgery, the First Central Clinical School, Tianjin Medical University, Tianjin 300192, China.
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Tan
authors.affiliation: Xin Jiao, Hai-Ning Zhi, Xiao-Man Liu, Chen-Hao Liu, Department of General Surgery, the First Central Clinical School, Tianjin Medical University, Tianjin 300192, China.
authors.forename: Hai-Ning
authors.initials: HN
authors.lastname: Zhi
authors.affiliation: Xin Jiao, Hai-Ning Zhi, Xiao-Man Liu, Chen-Hao Liu, Department of General Surgery, the First Central Clinical School, Tianjin Medical University, Tianjin 300192, China.
authors.forename: Xiao-Man
authors.initials: XM
authors.lastname: Liu
authors.affiliation: Xin Jiao, Hai-Ning Zhi, Xiao-Man Liu, Chen-Hao Liu, Department of General Surgery, the First Central Clinical School, Tianjin Medical University, Tianjin 300192, China.
authors.forename: Chen-Hao
authors.initials: CH
authors.lastname: Liu
authors.affiliation: Xin Jiao, Hai-Ning Zhi, Xiao-Man Liu, Chen-Hao Liu, Department of General Surgery, the First Central Clinical School, Tianjin Medical University, Tianjin 300192, China.
authors.forename: Da-Peng
authors.initials: DP
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D016009:Chi-Square Distribution; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007610:Kallikreins / Q000032:analysis* / Q000235:genetics; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D011237:Predictive Value of Tests; D016016:Proportional Hazards Models; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000401:mortality / Q000473:pathology / Q000628:therapy; D013997:Time Factors; D016896:Treatment Outcome; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; C100936:KLK10 protein, human; D007610:Kallikreins
keywords: Gastric cancer; Kallikrein gene 10; Prognostic biomarkers; Survival analysis