title: Metabonomic analysis reveals efficient ameliorating effects of acupoint stimulations on the menopause-caused alterations in mammalian metabolism.
abstract: Acupoint stimulations are effective in ameliorating symptoms of menopause which is an unavoidable ageing consequence for women. To understand the mechanistic aspects of such treatments, we systematically analyzed the effects of acupoint laser-irradiation and catgut-embedding on the ovariectomy-induced rat metabolic changes using NMR and GC-FID/MS methods. Results showed that ovariectomization (OVX) caused comprehensive metabolic changes in lipid peroxidation, glycolysis, TCA cycle, choline and amino acid metabolisms. Both acupoint laser-irradiation and catgut-embedding ameliorated the OVX-caused metabonomic changes more effectively than hormone replacement therapy (HRT) with nilestriol. Such effects of acupoint stimulations were highlighted in alleviating lipid peroxidation, restoring glucose homeostasis and partial reversion of the OVX-altered amino acid metabolism. These findings provided new insights into the menopause effects on mammalian biochemistry and beneficial effects of acupoint stimulations in comparison with HRT, demonstrating metabonomics as a powerful approach for potential applications in disease prognosis and developments of effective therapies.
authors.affiliation: CAS Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Centre for Magnetic Resonance, Biospectroscopy and Metabonomics Centre, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China.
authors.forename: Limin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: 1] CAS Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Centre for Magnetic Resonance, Biospectroscopy and Metabonomics Centre, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China [2] Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, P. R. China.
authors.forename: Yulan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Clinical Medical College of Acupuncture and Rehabilitation, Guangzhou University of Chinese Medicine, Guangzhou, 510405, P. R. China.
authors.forename: Yunxiang
authors.initials: Y
authors.lastname: Xu
authors.affiliation: CAS Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Centre for Magnetic Resonance, Biospectroscopy and Metabonomics Centre, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China.
authors.forename: Hehua
authors.initials: H
authors.lastname: Lei
authors.affiliation: CAS Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Centre for Magnetic Resonance, Biospectroscopy and Metabonomics Centre, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: CAS Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Centre for Magnetic Resonance, Biospectroscopy and Metabonomics Centre, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China.
authors.forename: Huihui
authors.initials: H
authors.lastname: Li
authors.affiliation: Affiliated BaoAn TCM Hospital, Guangzhou University of Chinese Medicine, Shenzhen, 518101, China.
authors.forename: Xiaosheng
authors.initials: X
authors.lastname: Lin
authors.affiliation: Affiliated BaoAn TCM Hospital, Guangzhou University of Chinese Medicine, Shenzhen, 518101, China.
authors.forename: Guizhen
authors.initials: G
authors.lastname: Chen
authors.affiliation: 1] CAS Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Centre for Magnetic Resonance, Biospectroscopy and Metabonomics Centre, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China [2] State Key Laboratory of Genetic Engineering, Biospectroscopy and Metabolomics, School of Life Sciences, Fudan University, Shanghai 200433, P. R. China.
authors.forename: Huiru
authors.initials: H
authors.lastname: Tang
mesh_terms: D015669:Acupuncture Points; D000818:Animals; D019963:Clinical Chemistry Tests; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D015227:Lipid Peroxidation; D008593:Menopause; D055432:Metabolomics; D023421:Models, Animal; D019906:Nuclear Magnetic Resonance, Biomolecular; D010052:Ovariectomy; D051381:Rats