title: Protein biotinylation.
abstract: Biotin is a naturally occurring vitamin that binds with high affinity to avidin and streptavidin proteins. Because biotin is small (244 Da), it can be conjugated to many proteins without altering their biological activities. The biotinylated molecule can be detected in ELISA, dot blot, or Western blot methods (see Western Blotting using Chemiluminescent Substrates) using streptavidin or avidin probes.
authors.affiliation: Thermo Fisher Scientific, Rockford, IL, USA. Electronic address: alice.schaffer@thermofisher.com.
authors.forename: Alice
authors.initials: A
authors.lastname: Alegria-Schaffer
mesh_terms: D001710:Biotin / Q000031:analogs & derivatives / Q000737:chemistry; D019921:Biotinylation; D011092:Polyethylene Glycols / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins; C513507:hydroxysuccinimidyl-poly(ethylene glycol)-biotin; D011092:Polyethylene Glycols; D001710:Biotin
keywords: Antibodies; NHS-PEG(4)-biotin; Phosphate-buffered Saline (PBS); Polyethylene glycol (PEG); Protein biotinylation