title: Hydroxycinnamic acids in Crepidiastrum denticulatum protect oxidative stress-induced retinal damage.
abstract: We investigated the effects of an ethanol extract of C. denticulatum (EECD) in a mouse model of glaucoma established by optic nerve crush (ONC), and found that EECD significantly protected against retinal ganglion cell (RGC) death caused by ONC. Furthermore, EECD effectively protected against N-methyl-d-aspartate-induced damage to the rat retinas. In vitro, EECD attenuated transformed retinal ganglion cell (RGC-5) death and significantly blunted the up-regulation of apoptotic proteins and mRNA level induced by 1-buthionine-(S,R)-sulfoximine combined with glutamate, reduced reactive oxygen species production by radical species, and inhibited lipid peroxidation. The major EECD components were found to be chicoric acid and 3,5-dicaffeoylquinic acid (3,5-DCQA) that have shown beneficial effects on retinal degeneration both in vitro and in vivo studies. Thus, EECD could be used as a natural neuroprotective agent for glaucoma, and chicoric acid and 3,5-DCQA as mark compounds for the development of functional food.
authors.affiliation: Functional Food Center, Korea Institute of Science and Technology (KIST) , Daejeon-dong, Gangneung 210-340, Republic of Korea.
authors.forename: Hong Ryul
authors.initials: HR
authors.lastname: Ahn
authors.forename: Hee Ju
authors.initials: HJ
authors.lastname: Lee
authors.forename: Kyung-A
authors.initials: KA
authors.lastname: Kim
authors.forename: Chul Young
authors.initials: CY
authors.lastname: Kim
authors.forename: Chu Won
authors.initials: CW
authors.lastname: Nho
authors.forename: Holim
authors.initials: H
authors.lastname: Jang
authors.forename: Cheol-Ho
authors.initials: CH
authors.lastname: Pan
authors.forename: Chang Yong
authors.initials: CY
authors.lastname: Lee
authors.forename: Sang Hoon
authors.initials: SH
authors.lastname: Jung
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D019659:Asteraceae / Q000737:chemistry*; D002109:Caffeic Acids / Q000008:administration & dosage / Q000032:analysis; D002726:Chlorogenic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000032:analysis; D003373:Coumaric Acids / Q000008:administration & dosage* / Q000032:analysis*; D004195:Disease Models, Animal; D005901:Glaucoma / Q000209:etiology / Q000517:prevention & control; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D016202:N-Methylaspartate / Q000008:administration & dosage; D009409:Nerve Crush; D018696:Neuroprotective Agents; D009900:Optic Nerve; D018384:Oxidative Stress; D010936:Plant Extracts / Q000008:administration & dosage; D051381:Rats; D017207:Rats, Sprague-Dawley; D012164:Retinal Diseases / Q000209:etiology / Q000517:prevention & control*; D013386:Succinates / Q000008:administration & dosage / Q000032:analysis
chemical_list: D002109:Caffeic Acids; D003373:Coumaric Acids; D018696:Neuroprotective Agents; D010936:Plant Extracts; D013386:Succinates; D002726:Chlorogenic Acid; D016202:N-Methylaspartate; C100434:3,5-dicaffeoylquinic acid; C100435:chicoric acid