title: Molecular Cloning and Expression Analysis of Triticale Phytocystatins During Development and Germination of Seeds.
abstract: Three triticale cDNAs encoding inhibitors of cysteine endopeptidases, belonging to phytocystatins, have been identified and designated asTrcC-1,TrcC-4andTrcC-5. Full-length cDNAs ofTrcC-1(617 bp) andTrcC-4(940 bp), as well as a fragment ofTrcC-5cDNA (369 bp), were obtained. A high-level identity of the deduced amino acid sequence of TrcCs with other known phytocystatins, especially with wheat and barley, has been observed. Moreover, the presence of conserved domain, containing the G and W residues, the sequence of QxVxG and the sequence of LARFAV, characteristic for plant cysteine endopeptidase inhibitors, has been noted. The profiles ofTrcC-1andTrcC-5mRNA levels in the developing seeds of two triticale cultivars that differ in their resistance to preharvest sprouting (Zorro and Disco) were similar. However, the expression ofTrcC-4was, higher in the developing seeds, and in the scutellum of germinating seeds of a cultivar more resistant to preharvest sprouting (Zorro) than in the less resistant (Disco). Additionally, the expression ofTrcC-4remained longer in developing seeds of Zorro as compared to Disco. The performed studies suggest that TrcC-4 might have an influence on the higher resistance of Zorro cultivar to preharvest sprouting.
authors.forename: Joanna
authors.initials: J
authors.lastname: Szewińska
authors.forename: Edyta
authors.initials: E
authors.lastname: Zdunek-Zastocka
authors.forename: Mirosław
authors.initials: M
authors.lastname: Pojmaj
authors.forename: Wiesław
authors.initials: W
authors.lastname: Bielawski
keywords: Germination; Phytocystatin; Preharvest sprouting; Seed development; Triticale