title: Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from glucose with elevated 3-hydroxyvalerate fraction via combined citramalate and threonine pathway in Escherichia coli.
abstract: The biosynthesis of polyhydroxyalkanoate copolymers in Escherichia coli from unrelated carbon sources becomes attractive nowadays. We previously developed a poly(hydroxybutyrate-co-hydroxyvalerte) (PHBV) biosynthetic pathway from an unrelated carbon source via threonine metabolic route in E. coli (Chen et al., Appl Environ Microbiol 77:4886-4893, 2011). In our study, a citramalate pathway was introduced in recombinant E. coli by cloning a cimA gene from Leptospira interrogans. By blocking the pyruvate and the propionyl-CoA catabolism and replacing the β-ketothiolase gene, the PHBV with 11.5 mol% 3HV fraction was synthesized. Further, the combination of citramalate pathway with the threonine biosynthesis pathway improved the 3HV fraction in PHBV copolymer to 25.4 mol% in recombinant E. coli.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, People's Republic of China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Liu
authors.forename: Qingsheng
authors.initials: Q
authors.lastname: Qi
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D007921:Leptospira interrogans / Q000201:enzymology / Q000235:genetics; D008293:Malates / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods*; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D010421:Pentanoic Acids / Q000378:metabolism; D011091:Polyesters / Q000378:metabolism*; D019289:Pyruvic Acid / Q000378:metabolism; D013912:Threonine / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
chemical_list: D000214:Acyl Coenzyme A; D005947:Glucose; D008293:Malates; D010421:Pentanoic Acids; D011091:Polyesters; D019289:Pyruvic Acid; D013912:Threonine; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C013056:beta-hydroxyvaleric acid; C009061:propionyl-coenzyme A; C011729:citramalate