title: [Relationship between bronchoalveolar lavage mycoplasma load and clinical characteristics in children with Mycoplasma pneumoniae pneumonia].
abstract: OBJECTIVE
  Mycoplasma pneumoniae (MP) is an important pathogen for community-acquired pneumonia in children. MP infection was considered to be self-limited, but many severe refractory MP pneumonia cases have been reported in recent years. The reason for variation in severity of MP pneumonia remains unclear. MP virulence including drug-resistance and host immunologic function are important influencing factors. The present study aimed to clarify relationship between local MP load and severity of MP pneumonia.
  
  
  METHODS
  MP DNA was quantitatively detected by fluorescent real-time PCR in bronchoalveolar lavage fluid (BALF) from 77 children with MP pneumonia. They were classified into groups of low MP load ( < 10(3)/ml, n = 14) , moderate MP load (10(3)-10(6)/ml, n = 22) and high MP load ( > 10(6)/ml, n = 41) . Clinical symptoms, main laboratory and imaging results of children among the three groups were compared.
  
  
  RESULTS
  When compared with low load group and moderate load group, high load group had longer fever duration (7 d, 10 d vs. 12 d) , longer time to normalization of temperature with macrolide administration (4 d, 8 d vs. 10 d) , more patients with high fever (50.0%, 68.2% vs. 87.8%) and longer duration of fever than 10 d (35.7%, 50.0% vs. 73.2%).Statistically significant difference existed in CRP among the three groups (1.0 mg/L, 11.5 mg/L, 34 mg/L). Large field of consolidation or atelectasis were found in 58.5% of high load patients, much higher than 22.7% in moderate load and 14.3% in low load patients. Bilateral or massive pleural effusion was not found in low load group, while in moderate load and high load group, they were 13.6% and 24.4%. However, no significant difference was found in symptoms and main laboratory and imaging results among different age groups in high load patients.
  
  
  CONCLUSIONS
  There is a close relationship between MP load in BALF and clinical characteristics in children with MP pneumonia. Those with high MP load have a more severe process.
authors.affiliation: Department of Pulmonology, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
authors.forename: Yun-gai
authors.initials: YG
authors.lastname: Cheng
authors.affiliation: Department of Pulmonology, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
authors.forename: Shu-xian
authors.initials: SX
authors.lastname: Li
authors.affiliation: Department of Pulmonology, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
authors.forename: Xue-jing
authors.initials: XJ
authors.lastname: Li
authors.affiliation: Department of Pulmonology, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Pulmonology, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China. Email: drchenzm@163.com.
authors.forename: Zhi-min
authors.initials: ZM
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D058491:Bacterial Load; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology*; D002648:Child; D002675:Child, Preschool; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D009177:Mycoplasma pneumoniae / Q000235:genetics / Q000302:isolation & purification*; D011019:Pneumonia, Mycoplasma / Q000382:microbiology* / Q000473:pathology*; D060888:Real-Time Polymerase Chain Reaction; D012720:Severity of Illness Index
chemical_list: D004269:DNA, Bacterial