title: The mitochondrial genome of the black dwarf honey bee, Apis andreniformis (Hymenoptera: Apidae).
abstract: We sequenced 17,329 bp of the black dwarf honey bee, Apis andreniformis (Hymenoptera: Apidae) mitochondrial genome (mitogenome) that lacked ∼200 bp of the A+T-rich region for the complete genomic sequence. The gene arrangement of the A. andreniformis mitogenome was identical to that of A. cerana. However, the genome contained five additional tRNALeu(CUN); four copies were located between tRNAMet and tRNAGln, and one copy was between tRNAGln and tRNAAla, along with the typical sets of genes (13 protein-coding genes, 22 tRNAs, and 2 rRNAs) including regular tRNALeu(CUN) and the A+T-rich region (at least 923 bp). Only one copy of tRNALeu(CUN) differed by 1 bp from the other four copies of tRNALeu(CUN). Each additional tRNALeu(CUN) was followed by a nearly identical 68-bp long repeat sequence (95.6% identity). All 13 protein coding genes had typica start codons found in insect mitochondrial protein coding genes (two ATA, nine ATT and two ATG).
authors.affiliation: a College of Agriculture & Life Sciences, Chonnam National University , Gwangju , Republic of Korea and.
authors.forename: Ah Rha
authors.initials: AR
authors.lastname: Wang
authors.affiliation: a College of Agriculture & Life Sciences, Chonnam National University , Gwangju , Republic of Korea and.
authors.forename: Min Jee
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: a College of Agriculture & Life Sciences, Chonnam National University , Gwangju , Republic of Korea and.
authors.forename: Joo Young
authors.initials: JY
authors.lastname: Lee
authors.affiliation: b Department of Agricultural Biology , National Academy of Agricultural Science , RDA , Suwon , Republic of Korea.
authors.forename: Yong Soo
authors.initials: YS
authors.lastname: Choi
authors.affiliation: b Department of Agricultural Biology , National Academy of Agricultural Science , RDA , Suwon , Republic of Korea.
authors.forename: Ratna
authors.initials: R
authors.lastname: Thapa
authors.affiliation: a College of Agriculture & Life Sciences, Chonnam National University , Gwangju , Republic of Korea and.
authors.forename: Iksoo
authors.initials: I
authors.lastname: Kim
mesh_terms: D000818:Animals; D001482:Base Composition; D001516:Bees / Q000235:genetics*; D018387:Codon, Initiator; D023061:Gene Order; D054629:Genome, Mitochondrial; D019476:Insect Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D018387:Codon, Initiator; D019476:Insect Proteins
keywords: Apis andreniformis; mitochondrial genome; tRNALeu(CUN); tandem repeat sequences