title: Regulation of interleukin-6 in head and neck squamous cell carcinoma is related to papillomavirus infection.
abstract: The prevalence of head and neck squamous cell carcinoma (HNSCC) related to human papillomavirus (HPV) is increasing, unlike tobacco- and alcohol-associated cancers. To gain a clearer understanding of the molecular mechanisms implicated in HNSCC, depending on the presence or not of a viral sequence, we investigated the expression of proteins detected in the tumor regions of HNSCC patients. Twenty-two untreated HNSCC patients were selected according to the presence of HPV-16. For six patients, tumor and controlateral healthy tissues were tested for viral detection before quantitative proteomic analysis. After confirmation by Western blot, proteins were connected into a network, leading to investigate interleukin-6 (IL-6) by immunocytochemistry and ELISA. 41 ± 5% of proteins quantified by proteomics were differentially expressed in tumor compared with healthy regions. Among them, 36 proteins were retained as modulated in HPV-16 positive or negative tumors, including cytokeratins, tubulins, annexin A1, and serpin B1. Network analysis suggested a central role of IL-6, confirmed by overexpression of IL-6 in tumor tissues as in sera of HPV-negative HNSCC compared with HPV-16-positive tumors. This modulation may contribute to the survival and proliferation of cancer cells, although it was not related to tumor stage or to the level of HPV-16 DNA.
authors.affiliation: Plateau Protéome Necker, IFR 94, Université Paris Descartes , Paris, France.
authors.forename: Ida Chiara
authors.initials: IC
authors.lastname: Guerrera
authors.forename: Ivan
authors.initials: I
authors.lastname: Quetier
authors.forename: Rachid
authors.initials: R
authors.lastname: Fetouchi
authors.forename: Frederique
authors.initials: F
authors.lastname: Moreau
authors.forename: Christelle
authors.initials: C
authors.lastname: Vauloup-Fellous
authors.forename: Bouchra
authors.initials: B
authors.lastname: Lekbaby
authors.forename: Caroline
authors.initials: C
authors.lastname: Rousselot
authors.forename: Cerina
authors.initials: C
authors.lastname: Chhuon
authors.forename: Aleksander
authors.initials: A
authors.lastname: Edelman
authors.forename: Marine
authors.initials: M
authors.lastname: Lefevre
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Nicolas
authors.forename: Dina
authors.initials: D
authors.lastname: Kremsdorf
authors.forename: Jean
authors.initials: J
authors.lastname: Lacau Saint Guily
authors.forename: Patrick
authors.initials: P
authors.lastname: Soussan
mesh_terms: D000368:Aged; D001483:Base Sequence; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000150:complications / Q000378:metabolism* / Q000821:virology; D016022:Case-Control Studies; D015331:Cohort Studies; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006258:Head and Neck Neoplasms / Q000150:complications / Q000378:metabolism* / Q000821:virology; D052162:Human papillomavirus 16 / Q000302:isolation & purification; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000150:complications / Q000378:metabolism* / Q000821:virology; D060888:Real-Time Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D019562:Viral Load
chemical_list: D017931:DNA Primers; D015850:Interleukin-6