title: Cellular redox homeostasis in endothelial cells treated with nonmodified and Fenton-modified nanodiamond powders.
abstract: Diamond nanoparticles find numerous applications in pharmacy, medicine, cosmetics, and biotechnology. However, possible adverse cellular effects of diamond nanoparticle cells have been reported, which may limit their use. The aim of this study was to compare the effect of nonmodified diamond nanoparticles (D) and diamond nanoparticles modified by the Fenton reaction (D+OH) on human umbilical cord endothelial cells (HUVEC-ST). We found that both D and D+OH show time- and concentration-dependent cytotoxicity, inducing apoptosis and necrosis of HUVEC-ST. Interaction with D and D+OH also induced changes in the production of reactive oxygen and nitrogen species and changes in the level of glutathione and activities of antioxidant enzymes in the cells. These data demonstrate that diamond nanoparticles may induce oxidative stress in human endothelial cells, which contributes to their cytotoxic effects seen at higher concentrations of D and D+OH.
authors.affiliation: Department of Molecular Biophysics, University of Lodz, Pomorska, Lodz.
authors.forename: K
authors.initials: K
authors.lastname: Solarska-Ściuk
authors.forename: A
authors.initials: A
authors.lastname: Gajewska
authors.forename: J
authors.initials: J
authors.lastname: Skolimowski
authors.forename: A
authors.initials: A
authors.lastname: Gajek
authors.forename: G
authors.initials: G
authors.lastname: Bartosz
mesh_terms: D001672:Biocompatible Materials / Q000633:toxicity; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006706:Homeostasis / Q000187:drug effects*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects*; D006801:Humans; D058612:Nanodiamonds / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D026361:Reactive Nitrogen Species / Q000032:analysis / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D058612:Nanodiamonds; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species
keywords: apoptosis; biocompatibility; nanodiamond particles; reactive oxygen and nitrogen species