title: Random biopsy during high-resolution anoscopy increases diagnosis of anal high-grade squamous intraepithelial lesions.
abstract: OBJECTIVE
  Random biopsy (RB) of normal appearing cervix during colposcopy increases high-grade dysplasia (HSIL) diagnosis but has not been studied in high-resolution anoscopy (HRA), that is, colposcopy transferred to the anal canal. We investigated the utility of RB during HRA.
  
  
  METHODS
  At HRA, the anal canal was divided into 4 quadrants. Areas suspicious for HSIL had standard biopsy (SB); random biopsies were taken from quadrants without apparent HSIL. Inclusion required ≥1 RB. Two providers performed all procedures (S.E.G., >10 years experience; M.M.G. 3 years experience).
  
  
  RESULTS
  Overall, 391 participants enrolled (mean age, 44.7 years); most were male (87.2%), non-Hispanic (69.8%), white (62.7%), and HIV positive (72.9%). Of 1761 biopsies, 883 were RBs (mean, 2.26/participant). HSIL was identified in 252 lesions, and in 132 participants (33.8%). Thirty-two HSILs (12.7%) and 13 participants (9.8%) were diagnosed by RB. RB increased total HSILs identified per participant (mean, 0.65 vs. 0.56; P < 0.001) and participants with HSIL (P < 0.001). Histologically, HSIL diagnoses via SB were no more dysplastic than random biopsies (relative risk, 0.82; range, 0.37-1.8). In multivariable analysis, factors affecting adjusted relative risk (ARR) of HSIL with any biopsy were provider [S.E.G vs. M.M.G.; ARR, 5.9; 95% confidence interval (CI), 1.3 to 25.8] and oncogenic human papillomaviral infection (ARR, 24.3; 95% CI, 2.8 to 213.3). Risk of HSIL on RB alone in multivariate analysis was associated with HSIL via SB (ARR, 3.4; 95% CI, 1.6 to 7.1 or ARR, 1.4; 95% CI, 1.1 to 1.9 per standard HSIL). Provider, HIV status, detectable viral load, age, or prior screening for or treatment of HSIL did not affect the utility of RB.
  
  
  CONCLUSIONS
  Addition of RB to HRA significantly increased both the number of HSILs and participants with HSIL identified.
authors.affiliation: Icahn School of Medicine at Mount Sinai, New York, NY.
authors.forename: Richard
authors.initials: R
authors.lastname: Silvera
authors.forename: Michael M
authors.initials: MM
authors.lastname: Gaisa
authors.forename: Stephen E
authors.initials: SE
authors.lastname: Goldstone
mesh_terms: D000328:Adult; D000368:Aged; D001003:Anal Canal / Q000473:pathology*; D001005:Anus Neoplasms / Q000175:diagnosis* / Q000209:etiology / Q000473:pathology; D001706:Biopsy / Q000379:methods*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000209:etiology / Q000473:pathology; D005260:Female; D015658:HIV Infections / Q000150:complications / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D011351:Proctoscopy / Q000379:methods; D011446:Prospective Studies; D055815:Young Adult