title: Molecular characterization of a cellulose synthase gene (AaxmCesA1) isolated from anAcacia auriculiformisxAcacia mangiumhybrid.
abstract: Cellulose is the major component of plant cell walls, providing mechanical strength to the structural framework of plants. In association with lignin, hemicellulose, protein and pectin, cellulose forms the strong yet flexible bio-composite tissue of wood. Wood formation is an essential biological process and is of significant importance to the cellulosic private sector industry. Cellulose synthase genes encode the catalytic subunits of a large protein complex responsible for the biogenesis of cellulose in higher plants. The hybridAcacia auriculiformisxAcacia mangiumrepresents an important source of tree cellulose for forest-based product manufacturing, with enormous economic potential. In this work, we isolate the first cellulose synthase gene, designatedAaxmCesA1, from this species. The isolated full-lengthAaxmCesA1cDNA encodes a polypeptide of 1,064 amino acids. Sequence analyses revealed thatAaxmCesA1cDNA possesses the key motif characteristics of a CesA protein. AaxmCesA1 shares more than 75 % amino acid sequence identity with CesA proteins from other plant species. Subsequently, the full-lengthAaxmCesA1gene of 7,389 bp with partial regulatory and 13 intron regions was also isolated. Relative gene expression analysis by quantitative PCR in different tissues of theAcaciahybrid, suggests the involvement of theAaxmCesA1gene in primary cell wall synthesis of rapidly dividing young root cells. Similarity analyses using Blast algorithms also suggests a role in primary cell wall deposition in theAcaciahybrid. Southern analysis predicts thatAaxmCesA1is a member of a multigene family with at least two isoforms in the genome of theAcaciahybrid.
authors.forename: Seok Yien Christina
authors.initials: SY
authors.lastname: Yong
authors.forename: Ratnam
authors.initials: R
authors.lastname: Wickneswari
keywords: Acacia hybrid; Cellulose synthase gene; Conserved motif; Isoform; Promoter region; Relative gene expression