title: Characterization and bioavailability of tea polyphenol nanoliposome prepared by combining an ethanol injection method with dynamic high-pressure microfluidization.
abstract: Tea polyphenols are major polyphenolic substances found in green tea with various biological activities. To overcome their instability toward oxygen and alkaline environments, tea polyphenol nanoliposome (TPN) was prepared by combining an ethanol injection method with dynamic high-pressure microfluidization. Good physicochemical characterizations (entrapment efficiency = 78.5%, particle size = 66.8 nm, polydispersity index = 0.213, and zeta potential = -6.16 mv) of TPN were observed. Compared with tea polyphenol solution, TPN showed equivalent antioxidant activities, indicated by equal DPPH free radical scavenging and slightly lower ferric reducing activities and lower inhibitions against Staphylococcus aureus , Escerhichia coli , Salmonella typhimurium , and Listeria monocytogenes . In addition, a relatively good sustained release property was observed in TPN, with only 29.8% tea polyphenols released from nanoliposome after 24 h of incubation. Moreover, TPN improved the stability of tea polyphenol in alkaline solution. This study expects to provide theories and practice guides for further applications of TPN.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University , No. 235 Nanjing East Road, Nanchang 330047, Jiangxi, China.
authors.forename: Li-qiang
authors.initials: LQ
authors.lastname: Zou
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Wei-lin
authors.initials: WL
authors.lastname: Liu
authors.forename: Rui-hong
authors.initials: RH
authors.lastname: Liang
authors.forename: Ti
authors.initials: T
authors.lastname: Li
authors.forename: Cheng-mei
authors.initials: CM
authors.lastname: Liu
authors.forename: Yan-lin
authors.initials: YL
authors.lastname: Cao
authors.forename: Jing
authors.initials: J
authors.lastname: Niu
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D001682:Biological Availability; D004355:Drug Stability; D000431:Ethanol; D008081:Liposomes; D053769:Nanocapsules; D010316:Particle Size; D059808:Polyphenols / Q000008:administration & dosage* / Q000493:pharmacokinetics* / Q000494:pharmacology; D011312:Pressure; D013662:Tea / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D008081:Liposomes; D053769:Nanocapsules; D059808:Polyphenols; D013662:Tea; D000431:Ethanol