title: Photodynamic inactivation of bioluminescent Escherichia coli by neutral and cationic pyrrolidine-fused chlorins and isobacteriochlorins.
abstract: Photodynamic inactivation of bioluminescent Escherichia coli in the presence of cationic chlorin and isobacteriochlorin photosensitizers (PSs) obtained from 5,10,15,20-tetrakis(pentafluorophenyl)-porphyrin is described. The spectroscopic data for the neutral and cationic derivatives and their photophysical characterizations, especially fluorescence and singlet oxygen generation capacity are also reported. The results show that there is a direct relation between the inactivation efficiency and the increasing number of charges on the molecules. The combined effect of higher wavelength absorption and number of positive charges on the PS shows a 6.1 log reduction during the inactivation process. Overall this study shows that the cationic isobacteriochlorin has high potential to be used as PS for the inactivation of Gram (-) bacteria.
authors.affiliation: Department of Chemistry & QOPNA, University of Aveiro, 3810-193 Aveiro, Portugal.
authors.forename: Mariana Q
authors.initials: MQ
authors.lastname: Mesquita
authors.affiliation: Department of Chemistry & QOPNA, University of Aveiro, 3810-193 Aveiro, Portugal. Electronic address: josemenezes@ua.pt.
authors.forename: José C J M D S
authors.initials: JC
authors.lastname: Menezes
authors.affiliation: Department of Chemistry & QOPNA, University of Aveiro, 3810-193 Aveiro, Portugal.
authors.forename: Maria G P M S
authors.initials: MG
authors.lastname: Neves
authors.affiliation: Department of Chemistry & QOPNA, University of Aveiro, 3810-193 Aveiro, Portugal.
authors.forename: Augusto C
authors.initials: AC
authors.lastname: Tomé
authors.affiliation: Department of Chemistry & QOPNA, University of Aveiro, 3810-193 Aveiro, Portugal.
authors.forename: José A S
authors.initials: JA
authors.lastname: Cavaleiro
authors.affiliation: Department of Biology & CESAM, University of Aveiro, 3810-193 Aveiro, Portugal.
authors.forename: Ângela
authors.initials: Â
authors.lastname: Cunha
authors.affiliation: Department of Biology & CESAM, University of Aveiro, 3810-193 Aveiro, Portugal.
authors.forename: Adelaide
authors.initials: A
authors.lastname: Almeida
authors.affiliation: Institut für Physik, Humboldt-Universität zu Berlin, Newtonstrasse 15, 12489 Berlin, Germany.
authors.forename: Steffen
authors.initials: S
authors.lastname: Hackbarth
authors.affiliation: Institut für Physik, Humboldt-Universität zu Berlin, Newtonstrasse 15, 12489 Berlin, Germany.
authors.forename: Beate
authors.initials: B
authors.lastname: Röder
authors.affiliation: Department of Chemistry & QOPNA, University of Aveiro, 3810-193 Aveiro, Portugal. Electronic address: faustino@ua.pt.
authors.forename: M Amparo F
authors.initials: MA
authors.lastname: Faustino
mesh_terms: D002412:Cations; D004926:Escherichia coli / Q000187:drug effects*; D008027:Light; D057230:Limit of Detection; D008164:Luminescent Proteins; D050296:Microbial Viability / Q000187:drug effects*; D017319:Photosensitizing Agents / Q000737:chemistry / Q000494:pharmacology; D011166:Porphyrins / Q000737:chemistry* / Q000494:pharmacology*; D011759:Pyrrolidines / Q000737:chemistry / Q000494:pharmacology
chemical_list: D002412:Cations; D008164:Luminescent Proteins; D017319:Photosensitizing Agents; D011166:Porphyrins; D011759:Pyrrolidines; C452434:isobacteriochlorin; C006969:chlorin; C032519:pyrrolidine
keywords: Cationic photosensitizers; Chlorin; Escherichia coli; Isobacteriochlorin; Photodynamic inactivation