title: Correlation of laryngeal squamous cell carcinoma and infections with either HHV-8 or HPV-16/18.
abstract: Each year more than 159,000 new cases of laryngeal cancer are diagnosed globally, and more than 9000 patients die due to this malignancy. Viral infections are a known risk factor for this malignancy. Thus, this study aimed to evaluate the role of HPV-16/18 and HHV-8 infection in patients with laryngeal cancer. In this case-control study, 60 formalin-fixed, paraffin-embedded samples of laryngeal cancer and 22 normal larynx tissue samples from the Pathology Department of Qaem Hospital, Mashhad, Iran were studied. After validating the diagnosis, the samples were evaluated for the detection of HPV-16/18 and HHV-8 DNA using PCR technique. The data were registered and analyzed using SPSS 18.0. The average age for patients and controls was 61.29±11.89 and 55.77±10.10, respectively. Fifty-four patients (90%) and 16 (72.7%) controls were male. PCR results detected no HPV-16/18 DNA in both groups. Although there were 2 positive HHV-8 samples in both laryngeal cancer and normal larynx samples, no significant relation was present (p=0.291). We found no significant relationship between infection with HHV-8 or HPV-16/18 and the existence of laryngeal cancer. However, more complementary studies are required to re-evaluate our results using more samples and better viral detection techniques.
authors.affiliation: Department of Pathology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Nema
authors.initials: N
authors.lastname: Mohamadian Roshan
authors.affiliation: Department of Pathology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: Jafarianah@mums.ac.ir.
authors.forename: Amirhossein
authors.initials: A
authors.lastname: Jafarian
authors.affiliation: Cancer Molecular Pathology Research Center, Department of Hematology and Blood Bank, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Ayatollahi
authors.affiliation: Department of Microbiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Kiarash
authors.initials: K
authors.lastname: Ghazvini
authors.affiliation: Department of Pathology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Seyyed Abbas
authors.initials: SA
authors.lastname: Tabatabaee
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000821:virology*; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006566:Herpesviridae Infections / Q000150:complications* / Q000453:epidemiology; D019288:Herpesvirus 8, Human; D052162:Human papillomavirus 16; D052161:Human papillomavirus 18; D006801:Humans; D007822:Laryngeal Neoplasms / Q000821:virology*; D008297:Male; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000150:complications* / Q000453:epidemiology; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral
keywords: HHV-8; HPV-16; HPV-18; Laryngeal cancer