title: Identification of a plant receptor for extracellular ATP.
abstract: Extracellular adenosine 5'-triphosphate (ATP) is an essential signaling molecule that is perceived in mammals by plasma membrane P2-type purinoceptors. Similar ATP receptors do not exist in plants, although extracellular ATP has been shown to play critical roles in plant growth, development, and stress responses. Here, we identify an ATP-insensitive Arabidopsis mutant, dorn1 (Does not Respond to Nucleotides 1), defective in lectin receptor kinase I.9 (Arabidopsis Information Resource accession code At5g60300). DORN1 binds ATP with high affinity (dissociation constant of 45.7 ± 3.1 nanomolar) and is required for ATP-induced calcium response, mitogen-activated protein kinase activation, and gene expression. Ectopic expression of DORN1 increased the plant response to physical wounding. We propose that DORN1 is essential for perception of extracellular ATP and likely plays a variety of roles in plant stress resistance.
authors.affiliation: Divisions of Biochemistry and Plant Sciences, Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA.
authors.forename: Jeongmin
authors.initials: J
authors.lastname: Choi
authors.forename: Kiwamu
authors.initials: K
authors.lastname: Tanaka
authors.forename: Yangrong
authors.initials: Y
authors.lastname: Cao
authors.forename: Yue
authors.initials: Y
authors.lastname: Qi
authors.forename: Jing
authors.initials: J
authors.lastname: Qiu
authors.forename: Yan
authors.initials: Y
authors.lastname: Liang
authors.forename: Sang Yeol
authors.initials: SY
authors.lastname: Lee
authors.forename: Gary
authors.initials: G
authors.lastname: Stacey
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D004789:Enzyme Activation; D018506:Gene Expression Regulation, Plant; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009154:Mutation; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D011975:Receptors, Mitogen / Q000235:genetics / Q000378:metabolism*; D018048:Receptors, Purinergic P2 / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological / Q000235:genetics / Q000502:physiology*; D015854:Up-Regulation
chemical_list: D029681:Arabidopsis Proteins; D011975:Receptors, Mitogen; D018048:Receptors, Purinergic P2; D000255:Adenosine Triphosphate; C586088:LecRK-I.9 protein, Arabidopsis; D011494:Protein Kinases; D020928:Mitogen-Activated Protein Kinases