title: Detecting Concentration of Analytes with DETECHIP: A Molecular Sensing Array.
abstract: DETECHIP®is a detection system made of various sensors that has been shown to detect and discriminate between small molecules of interest, including various illicit and over-the-counter drugs. Previously, detection was normalized to a single concentration of analyte. Now this detection assay can detect concentration differences in analytes via red, green, and blue color value changes and shifts in the UV-Vis spectra of the assay. To determine the concentrations differences, the exposed assays were scanned on a flatbed scanner and the images were analyzed for individual RGB values with a custom macro in ImageJ, an image analysis program. Increasing concentrations of the analyte resulted in greater differences in color values between control and analyte wells. These differences showed a linear relationship to concentration change, some with correlation coefficients greater than 98%. This work expands the capability of DETECHIP to give information about the concentration of analyte when the analyte identity is known.
authors.affiliation: Department of Chemistry, Doane College, Crete, USA.
authors.forename: Hannah
authors.initials: H
authors.lastname: Johnke
authors.affiliation: Department of Chemistry, Doane College, Crete, USA.
authors.forename: Gary
authors.initials: G
authors.lastname: Batres
authors.affiliation: Department of Chemistry, Doane College, Crete, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Wilson
authors.affiliation: Department of Chemistry, Doane College, Crete, USA.
authors.forename: Andrea E
authors.initials: AE
authors.lastname: Holmes
authors.affiliation: Department of Chemistry, Doane College, Crete, USA.
authors.forename: Sharmin
authors.initials: S
authors.lastname: Sikich
keywords: Analyte Concentration; Colorimetric Arrays; Drug Detection; RGB Analysis; Sensors