title: Conformation and lipid interaction of the fusion peptide of the paramyxovirus PIV5 in anionic and negative-curvature membranes from solid-state NMR.
abstract: Viral fusion proteins catalyze the merger of the virus envelope and the target cell membrane through multiple steps of protein conformational changes. The fusion peptide domain of these proteins is important for membrane fusion, but how it causes membrane curvature and dehydration is still poorly understood. We now use solid-state NMR spectroscopy to investigate the conformation, topology, and lipid and water interactions of the fusion peptide of the PIV5 virus F protein in three lipid membranes, POPC/POPG, DOPC/DOPG, and DOPE. These membranes allow us to investigate the effects of lipid chain disorder, membrane surface charge, and intrinsic negative curvature on the fusion peptide structure. Chemical shifts and spin diffusion data indicate that the PIV5 fusion peptide is inserted into all three membranes but adopts distinct conformations: it is fully α-helical in the POPC/POPG membrane, adopts a mixed strand/helix conformation in the DOPC/DOPG membrane, and is primarily a β-strand in the DOPE membrane. (31)P NMR spectra show that the peptide retains the lamellar structure and hydration of the two anionic membranes. However, it dehydrates the DOPE membrane, destabilizes its inverted hexagonal phase, and creates an isotropic phase that is most likely a cubic phase. The ability of the β-strand conformation of the fusion peptide to generate negative Gaussian curvature and to dehydrate the membrane may be important for the formation of hemifusion intermediates in the membrane fusion pathway.
authors.affiliation: Department of Chemistry, Iowa State University , Ames, Iowa 50011 United States.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Yao
authors.forename: Mei
authors.initials: M
authors.lastname: Hong
mesh_terms: D000595:Amino Acid Sequence; D000838:Anions; D008055:Lipids / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008561:Membrane Fusion; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000502:physiology; D014760:Viral Fusion Proteins / Q000737:chemistry* / Q000502:physiology
chemical_list: D000838:Anions; D008055:Lipids; D010455:Peptides; D014760:Viral Fusion Proteins