title: Fabrication and in vitro biocompatibility of biomorphic PLGA/nHA composite scaffolds for bone tissue engineering.
abstract: In this study, biomorphic poly(dl-lactic-co-glycolic acid)/nano-hydroxyapatite (PLGA/nHA) composite scaffolds were successfully prepared using cane as a template. The porous morphology, phase, compression characteristics and in vitro biocompatibility of the PLGA/nHA composite scaffolds and biomorphic PLGA scaffolds as control were investigated. The results showed that the biomorphic scaffolds preserved the original honeycomb-like architecture of cane and exhibited a bimodal porous structure. The average channel diameter and micropore size of the PLGA/nHA composite scaffolds were 164 ± 52 μm and 13 ± 8 μm, respectively, with a porosity of 89.3 ± 1.4%. The incorporation of nHA into PLGA decreased the degree of crystallinity of PLGA, and significantly improved the compressive modulus of biomorphic scaffolds. The in vitro biocompatibility evaluation with MC3T3-E1 cells demonstrated that the biomorphic PLGA/nHA composite scaffolds could better support cell attachment, proliferation and differentiation than the biomorphic PLGA scaffolds. The localization depth of MC3T3-E1 cells within the channels of the biomorphic PLGA/nHA composite scaffolds could reach approximately 400 μm. The results suggested that the biomorphic PLGA/nHA composite scaffolds were promising candidates for bone tissue engineering.
authors.affiliation: State Key Laboratory for Mechanical Behaviors of Materials, Xi'an Jiaotong University, Xi'an 710049, China. Electronic address: jmqian@mail.xjtu.edu.cn.
authors.forename: Junmin
authors.initials: J
authors.lastname: Qian
authors.affiliation: State Key Laboratory for Mechanical Behaviors of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Weijun
authors.initials: W
authors.lastname: Xu
authors.affiliation: State Key Laboratory for Mechanical Behaviors of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Xueqing
authors.initials: X
authors.lastname: Yong
authors.affiliation: State Key Laboratory for Mechanical Behaviors of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Xinxia
authors.initials: X
authors.lastname: Jin
authors.affiliation: State Key Laboratory for Mechanical Behaviors of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001842:Bone and Bones / Q000187:drug effects / Q000502:physiology*; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D048430:Cell Shape; D017886:Durapatite / Q000138:chemical synthesis*; D005455:Fluorescent Antibody Technique; D019344:Lactic Acid / Q000138:chemical synthesis* / Q000494:pharmacology; D008422:Materials Testing; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D011100:Polyglycolic Acid / Q000138:chemical synthesis* / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D017886:Durapatite; D000469:Alkaline Phosphatase
keywords: Biocompatibility; Biomimetic scaffold; Biomorphous; Bone tissue engineering; PLGA/nHA composite