title: Cloning, expression, purification and preliminary X-ray crystallographic analysis of mouse protein arginine methyltransferase 7.
abstract: Protein arginine methyltransferase 7 (PRMT7) is a unique but less characterized member of the family of protein arginine methyltransferases (PRMTs) that plays a role in male germline gene imprinting. PRMT7 is the only known PRMT member that catalyzes the monomethylation but not the dimethylation of the target arginine residues and harbours two catalytic domains in tandem. PRMT7 genes from five different species were cloned and expressed in Escherichia coli and Sf21 insect cells. Four gave soluble proteins from Sf21 cells, of which two were homogeneous and one gave crystals. The mouse PRMT7 structure was solved by the single anomalous dispersion method using a crystal soaked with thimerosal that diffracted to beyond 2.1 Å resolution. The crystal belonged to space group P4(3)2(1)2, with unit-cell parameters a = b = 97.4, c = 168.1 Å and one PRMT7 monomer in the asymmetric unit. The structure of another crystal form belonging to space group I222 was solved by molecular replacement.
authors.affiliation: Département de Biologie Structurale Intégrative, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), INSERM U964/CNRS UMR 7104/Université de Strasbourg, 1 Rue Laurent Fries, 67404 Illkirch, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Cura
authors.affiliation: Département de Biologie Structurale Intégrative, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), INSERM U964/CNRS UMR 7104/Université de Strasbourg, 1 Rue Laurent Fries, 67404 Illkirch, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Troffer-Charlier
authors.affiliation: Département de Biologie Structurale Intégrative, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), INSERM U964/CNRS UMR 7104/Université de Strasbourg, 1 Rue Laurent Fries, 67404 Illkirch, France.
authors.forename: Marie-Annick
authors.initials: MA
authors.lastname: Lambert
authors.affiliation: Département de Biologie Structurale Intégrative, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), INSERM U964/CNRS UMR 7104/Université de Strasbourg, 1 Rue Laurent Fries, 67404 Illkirch, France.
authors.forename: Luc
authors.initials: L
authors.lastname: Bonnefond
authors.affiliation: Département de Biologie Structurale Intégrative, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), INSERM U964/CNRS UMR 7104/Université de Strasbourg, 1 Rue Laurent Fries, 67404 Illkirch, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Cavarelli
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D029681:Arabidopsis Proteins / Q000378:metabolism; D002850:Chromatography, Gel; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008027:Light; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011484:Protein-Arginine N-Methyltransferases / Q000737:chemistry* / Q000302:isolation & purification*; D012542:Scattering, Radiation; D014162:Transfection
chemical_list: D029681:Arabidopsis Proteins; C570798:PRMT7 protein, mouse; D011484:Protein-Arginine N-Methyltransferases
keywords: DLS; insect cells; methyltransferases; thermal shift; thimerosal