title: Shotgun Analysis of the Secretome of Fusarium graminearum.
abstract: Fusarium head blight, caused predominately by Fusarium graminearum, is one of the most destructive diseases of wheat (Triticum aestivum L.) worldwide. To characterize the profile of proteins secreted by F. graminearum, the extracellular proteins were collectively obtained from F. graminearum culture supernatants and evaluated using one-dimensional SDS-PAGE and liquid chromatography-tandem mass spectrometry. A total of 87 proteins have been identified, of which 63 were predicted as secretory proteins including those with known functions. Meanwhile, 20 proteins that are not homologous to genomic sequences with known functions have also been detected. Some of the identified proteins are possible virulence factors and may play extracellular roles during F. graminearum infection. This study provides a valuable dataset of F. graminearum extracellular proteins, and a better understanding of the virulence mechanisms of the pathogen.
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, 271018 Shandong People's Republic of China.
authors.forename: Xian-Ling
authors.initials: XL
authors.lastname: Ji
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, 271018 Shandong People's Republic of China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, 271018 Shandong People's Republic of China.
authors.forename: Zai-Dong
authors.initials: ZD
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, 271018 Shandong People's Republic of China.
authors.forename: An-Fei
authors.initials: AF
authors.lastname: Li
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, 271018 Shandong People's Republic of China.
authors.forename: Ling-Rang
authors.initials: LR
authors.lastname: Kong
keywords: Fusarium graminearum; LC-MS/MS; Secretome; Triticum aestivum L.; Virulence