title: Relevance of the carnosic acid/carnosol ratio for the level of rosemary diterpene transfer and for improving lamb meat antioxidant status.
abstract: The aim of the present work was to evaluate whether the relation between the concentrations of the two major diterpenes present in two typified rosemary extracts affects their levels of deposition and antioxidant capacity in different lamb tissues. The composition of the extracts expressed as percentage of weight/weight was 1:1 (14-16)% and 2:1 (25-11)% (carnosic acid-carnosol), respectively. Thirty weaned lambs were assigned randomly to three homogeneous groups. One group was fed a basal diet as a control and the diets of the other two were enriched with rosemary extracts 1:1 and 2:1, respectively. HPLC-ESI-MS/TOF identified a metabolite (C19H22O3) described for the first time in lamb tissues, along with carnosol, carnosic acid, rosmanol and carnosol-p-quinone. The results obtained corroborate the importance of the presence of carnosol in the dietary administration of rosemary extract as a way of improving the stability of the diterpene fraction during feed manufacturing and the level of deposition and antioxidant efficacy of diterpenes after ruminal fermentation.
authors.affiliation: Murcia Institute of Agri-Food Research and Development (IMIDA), c/ Mayor s/n, La Alberca, 30150 Murcia, Spain. Electronic address: mariaj.jordan@carm.es.
authors.forename: María J
authors.initials: MJ
authors.lastname: Jordán
authors.affiliation: Research and Development Department, Nutrafur S.A., Alcantarilla, Murcia, Spain; Aging Research Institute, University of Murcia, Espinardo, 30071 Murcia, Spain.
authors.forename: Julián
authors.initials: J
authors.lastname: Castillo
authors.affiliation: Department of Food Science and Technology and Nutrition, Faculty of Veterinary Science, University of Murcia, Espinardo, 30071 Murcia, Spain.
authors.forename: Sancho
authors.initials: S
authors.lastname: Bañón
authors.affiliation: Murcia Institute of Agri-Food Research and Development (IMIDA), c/ Mayor s/n, La Alberca, 30150 Murcia, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Martínez-Conesa
authors.affiliation: Murcia Institute of Agri-Food Research and Development (IMIDA), c/ Mayor s/n, La Alberca, 30150 Murcia, Spain.
authors.forename: José A
authors.initials: JA
authors.lastname: Sotomayor
mesh_terms: D045784:Abietanes / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants; D004032:Diet; D008460:Meat / Q000032:analysis*; D027542:Rosmarinus / Q000737:chemistry*; D012756:Sheep; D017365:Spices
chemical_list: D045784:Abietanes; D000975:Antioxidants; C068623:carnosol
keywords: Antioxidant status; Carnosic acid; Carnosol; Lamb tissues; Level of transfer; Rosemary extract