title: Therapeutic effect of anti-αv integrin mAb on Theiler's murine encephalomyelitis virus-induced demyelinating disease.
abstract: We examined the regulatory role of αv integrins in the development of Theiler's murine encephalomyelitis virus-induced demyelinating disease (TMEV-IDD), a model of multiple sclerosis (MS). Blockade of αv integrins by anti-αv integrin monoclonal antibody (mAb) in the effector phase significantly suppressed the development of TMEV-IDD both clinically and histologically. The number of infiltrating mononuclear cells (MNCs) in the CNS was significantly decreased in mice treated with anti-αv integrin mAb. Flow cytometric analysis of cytokine staining revealed that absolute numbers of IFN-γ- and IL-17-producing CD4+ and IFN-γ-producing CD8+ T cells were significantly decreased in the CNS of mice treated with anti-αv integrin mAb. These data suggest that αv integrins may play important roles in the development of TMEV-IDD.
authors.affiliation: Department of Biomedical Laboratory Sciences, Graduate School of Medicine, Shinshu University, Matsumoto, Nagano 390-8621, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Tomiki
authors.affiliation: Department of Biomedical Laboratory Sciences, Graduate School of Medicine, Shinshu University, Matsumoto, Nagano 390-8621, Japan.
authors.forename: Tomoki
authors.initials: T
authors.lastname: Kaneyama
authors.affiliation: Department of Biomedical Laboratory Sciences, Graduate School of Medicine, Shinshu University, Matsumoto, Nagano 390-8621, Japan.
authors.forename: Kunitoshi
authors.initials: K
authors.lastname: Kobayashi
authors.affiliation: Department of Pediatrics, School of Medicine, Shinshu University, Matsumoto, Nagano 390-8621, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Inaba
authors.affiliation: Department of Biomedical Laboratory Sciences, Graduate School of Medicine, Shinshu University, Matsumoto, Nagano 390-8621, Japan.
authors.forename: Motoki
authors.initials: M
authors.lastname: Ichikawa
authors.affiliation: Department of Immunology, Juntendo University Graduate School of Medicine, Bunkyo-ku, Tokyo 113-8421, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Yagita
authors.affiliation: Department of Microbiology-Immunology, Northwestern University Feinberg Medical School, 303 East Chicago Avenue, Chicago, IL 60611, USA.
authors.forename: Byung S
authors.initials: BS
authors.lastname: Kim
authors.affiliation: Department of Biomedical Laboratory Sciences, Graduate School of Medicine, Shinshu University, Matsumoto, Nagano 390-8621, Japan. Electronic address: kshosei@shinshu-u.ac.jp.
authors.forename: Chang-Sung
authors.initials: CS
authors.lastname: Koh
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D018188:Cardiovirus Infections / Q000276:immunology* / Q000473:pathology; D020278:Demyelinating Autoimmune Diseases, CNS / Q000276:immunology* / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D039564:Integrin alphaV / Q000276:immunology*; D051379:Mice; D060888:Real-Time Polymerase Chain Reaction; D017945:Theilovirus
chemical_list: D000911:Antibodies, Monoclonal; D039564:Integrin alphaV
keywords: Demyelinating disease; Multiple sclerosis (MS); Theiler's murine encephalomyelitis virus-induced demyelinating disease (TMEV-IDD); αv integrin