title: [Antitumor effects of fresh gecko hydrolysate on H22 transplanted tumor in mice].
abstract: OBJECTIVE
  To compare the inhibitory effects of fresh gecko crude extract and its hydrolysate on H22 transplanted tumor in mice.
  
  
  METHODS
  The content of soluble nitrogen (SN-TCA index) was used to determine the degree of enzymolysis. The hydrolysate of gecko was obtained from fresh gecko crude extract by pepsin and papain hydrolyzing. H22 transplanted tumor mouse models were established and divided into negative group, positive group,crude extract group and hydrolysate group.
  
  
  RESULTS
  The inhibition rate of the H22 tumor-bearing mice was 29.17%, 48.99% respectively for the crude extract group and the hydrolysate group. The inhibition rate of hydrolysate group and the negative group were significantly different (P < 0.05). The spleen and thymus index for the crude extract group and the hydrolysate group didn't show different compared with the negative group.
  
  
  CONCLUSIONS
  The crude extract of the fresh gecko and the hydrolysate can inhibit the growth of the H22 transplanted tumor. The enzymolysis by pepsin and papain can increase the antitumor activity of the crude extract of fresh gecko.
authors.affiliation: Department of Biophamaceutics, School of Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. gxx060801509@126.com
authors.forename: Xiang-Xiang
authors.initials: XX
authors.lastname: Gu
authors.affiliation: Department of Biophamaceutics, School of Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Yun
authors.affiliation: Department of Biophamaceutics, School of Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Wang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D006868:Hydrolysis; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D008116:Lizards; D008419:Materia Medica / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D010434:Pepsin A / Q000378:metabolism; D011492:Protein Hydrolysates / Q000008:administration & dosage / Q000494:pharmacology*; D013154:Spleen / Q000187:drug effects; D013950:Thymus Gland / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D008419:Materia Medica; D011492:Protein Hydrolysates; D010434:Pepsin A