title: ATP-Binding Cassette Transporter B4 Anchors the Cell Adhesion Molecule DdCAD-1 to Cell Membrane in Dictyostelium discoideum.
abstract: In Dictyostelium, soluble cell adhesion molecule, DdCAD-1, regulates cell-cell interaction through an unknown anchoring protein on the plasma membrane. Far western blot analysis using different probes revealed that the potential DdCAD-1 interacting protein was between 64 and 98 kDa. To isolate and identify the anchoring protein, GST-DdCAD-1 and anchoring protein were cross-linked in vivo by chemical cross-linker and stable protein complex was isolated by co-immunoprecipitation assays. The protein cross-linked to DdCAD-1 was extracted from the gel slice and trypsinized. The peptides were subjected to analysis by mass spectrometry, which showed that the putative anchoring protein belongs to ATP-binding cassette transporter family.
authors.affiliation: School of Life Science, East China Normal University, 200062 Shanghai, China.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Yang
authors.affiliation: School of Life Science, East China Normal University, 200062 Shanghai, China.
authors.forename: Liansheng
authors.initials: L
authors.lastname: Hou
authors.affiliation: Banting and Best Department of Medical Research and the Department of Biochemistry, University of Toronto, Toronto, Ontario M5S 1A8 Canada.
authors.forename: Qixiu
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Banting and Best Department of Medical Research and the Department of Biochemistry, University of Toronto, Toronto, Ontario M5S 1A8 Canada.
authors.forename: Chi-Hung
authors.initials: CH
authors.lastname: Siu
keywords: ABC transporters; Anchoring protein; Co-immunoprecipitation; Cross-linking; DdCAD-1