title: Clinical laboratory urine analysis: comparison of the UriSed automated microscopic analyzer and the manual microscopy.
abstract: BACKGROUND
  Several automated urine sediment analyzers have been introduced to clinical laboratories. Automated microscopic pattern recognition is a new technique for urine particle analysis. We evaluated the analytical and diagnostic performance of the UriSed automated microscopic analyzer and compared with manual microscopy for urine sediment analysis.
  
  
  METHODS
  Precision, linearity, carry-over, and method comparison were carried out. A total of 600 urine samples sent for urinalysis were assessed using the UriSed automated microscopic analyzer and manual microscopy.
  
  
  RESULTS
  Within-run and between-run precision of the UriSed for red blood cells (RBC) and white blood cells (WBC) were acceptable at all levels (CV < 20%). Within-run and between-run imprecision of the UriSed testing for cast, squamous epithelial cells (EPI), and bacteria (BAC) were good at middle level and high level (CV < 20%). The linearity analysis revealed substantial agreement between the measured value and the theoretical value of the UriSed for RBC, WBC, cast, EPI, and BAC (r > 0.95). There was no carry-over. RBC, WBC, and squamous epithelial cells with sensitivities and specificities were more than 80% in this study.
  
  
  CONCLUSIONS
  There is substantial agreement between the UriSed automated microscopic analyzer and the manual microscopy methods. The UriSed provides for a rapid turnaround time.
authors.affiliation: Department of Clinical Laboratory, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
authors.forename: Junlong
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Clinical Laboratory, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
authors.forename: Chengbin
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
authors.forename: Jiaxin
authors.initials: J
authors.lastname: Yue
authors.affiliation: Department of Clinical Laboratory, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
authors.forename: Mianyang
authors.initials: M
authors.lastname: Li
authors.affiliation: Wenzhou Medical College, Wenzhou 325035, China.
authors.forename: Hongrui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of the 2008 Clinical Medicine, School of Basic Medical Sciences, Peking University, Beijing 100083, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Ma
authors.affiliation: Department of Clinical Laboratory, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
authors.forename: Xincui
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Xue
authors.affiliation: Department of Clinical Laboratory, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Qing
authors.affiliation: Beijing Biochem Hengye Science Technology Development Co. Ltd., Beijing 100020, China.
authors.forename: Shengjiang
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
authors.forename: Daijun
authors.initials: D
authors.lastname: Xiang
authors.affiliation: Department of Clinical Laboratory, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Cong
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001331:Automation; D005260:Female; D006801:Humans; D007753:Laboratories; D008297:Male; D008853:Microscopy / Q000379:methods*; D008875:Middle Aged; D015203:Reproducibility of Results; D016482:Urinalysis; D055815:Young Adult