title: MiR-200a is involved in proliferation and apoptosis in the human endometrial adenocarcinoma cell line HEC-1B by targeting the tumor suppressor PTEN.
abstract: Abnormal cell proliferation is a main driver of tumor formation and development, which involves the deletion, mutation, and downregulation of tumor suppressor genes. One study recently demonstrated that miR-200a plays an oncogenic role by inhibiting phosphatase and tensin homolog deleted on chromosome ten (PTEN) expression. In the human endometrial adenocarcinoma cell line HEC-1B, suppression of miR-200a expression inhibited cell proliferation and promoted apoptosis, whereas its over-expression had no effect on proliferation and apoptosis. Furthermore, inhibition or over-expression of miR-200a increased or reduced the expression of PTEN, respectively, with no change in PTEN mRNA levels. These effects were achieved by directly targeting miR-200a to the 3' untranslated region of the PTEN mRNA to inhibit its translation. Taken together, we propose that in HEC-1B cells, miR-200a functions as an oncogene, affecting proliferation and apoptosis by regulating the expression of the tumor suppressor PTEN at the translational level.
authors.affiliation: Laboratory of Reproductive Biology, Chongqing Medical University, No. 1 Yixueyuan Rd, Box 197, Chongqing, 400016, People's Republic of China, cqums.lirong@163.com.
authors.forename: Rong
authors.initials: R
authors.lastname: Li
authors.forename: Jun-Lin
authors.initials: JL
authors.lastname: He
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Chen
authors.forename: Chun-Lan
authors.initials: CL
authors.lastname: Long
authors.forename: De-Hui
authors.initials: DH
authors.lastname: Yang
authors.forename: Yu-Bin
authors.initials: YB
authors.lastname: Ding
authors.forename: Hong-Bo
authors.initials: HB
authors.lastname: Qi
authors.forename: Xue-Qing
authors.initials: XQ
authors.lastname: Liu
mesh_terms: D020413:3' Untranslated Regions; D000230:Adenocarcinoma / Q000235:genetics*; D017209:Apoptosis / Q000235:genetics*; D020029:Base Pairing; D001483:Base Sequence; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016889:Endometrial Neoplasms / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D051059:PTEN Phosphohydrolase / Q000737:chemistry / Q000235:genetics*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; C533896:MIRN200 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D051059:PTEN Phosphohydrolase