title: Cathodic electrochemiluminescence immunosensor based on nanocomposites of semiconductor carboxylated g-C3N4 and graphene for the ultrasensitive detection of squamous cell carcinoma antigen.
abstract: A novel label-free electrochemiluminescence (ECL) immunosensor was developed for the detection of squamous cell carcinoma antigen (SCCA) based on nanocomposites of semiconductor carboxylated graphitic carbon nitride (g-C3N4) and graphene (g-C3N4-graphene). The ECL intensity of carboxylated g-C3N4 was much enhanced after being combined with graphene which had excellent electron-transfer ability. The sensing platform was constructed by depositing g-C3N4-graphene on electrodes and immobilizing antibodies on the surface of carboxylated g-C3N4 through amidation. The specific immunoreaction between SCCA and antibody resulted in the decrease of ECL intensity and the intensity decreased linearly with the logarithm of SCCA concentration in the range of 0.025-10 ng mL(-1) with a detection limit of 8.53 pg mL(-1). The developed ECL immunosensor exhibited high sensitivity, good reproducibility and long-term stability, which possessed great potential for cancer detection in clinical laboratory diagnosis.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Xiaoyue
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Hongmin
authors.initials: H
authors.lastname: Ma
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wu
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Du
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China. Electronic address: sdjndxwq@163.com.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis* / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation; D003217:Conductometry / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006108:Graphite / Q000737:chemistry*; D007118:Immunoassay / Q000295:instrumentation*; D008163:Luminescent Measurements / Q000295:instrumentation*; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D009570:Nitriles / Q000737:chemistry*; D015203:Reproducibility of Results; D012666:Semiconductors; D012680:Sensitivity and Specificity; D015843:Serpins / Q000032:analysis* / Q000276:immunology
chemical_list: D000951:Antigens, Neoplasm; D009570:Nitriles; D015843:Serpins; C051072:squamous cell carcinoma-related antigen; C011206:cyanogen; D006108:Graphite
keywords: Carboxylated graphitic carbon nitride; Electrochemiluminescence immunosensor; Graphene; Squamous cell carcinoma antigen