title: Evaluation of the specific adsorption of biotinylated microbubbles using a quartz crystal microbalance.
abstract: Specific adsorption of biotinylated microbubbles to streptavidin was evaluated by measuring the resonant frequency of an AT-cut quartz crystal microbalance (QCM). Streptavidin was fixed via self-assembled monolayers coated onto the QCM electrode. The resonant frequency of the QCM decreased as a result of specific adsorption of the biotinylated microbubbles, compared with the results for microbubbles containing no biotin. Additionally, there was significant evidence indicating that the frequency shift was caused by the internal gas of the microbubble, as well as the mass of the outer-shell material surrounding the gas. These results suggest that the QCM measurement system can be used effectively to evaluate the specific adsorption of targeted microbubbles.
authors.affiliation: Faculty of Life and Medical Sciences, Doshisha University, Kyoto, Japan. Electronic address: buj3076@gmail.com.
authors.forename: Takashige
authors.initials: T
authors.lastname: Muramoto
authors.affiliation: Faculty of Science and Engineering, Doshisha University, Kyoto, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Shimoya
authors.affiliation: Faculty of Life and Medical Sciences, Doshisha University, Kyoto, Japan; Center for Frontier Medical Engineering, Chiba University, Chiba, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Yoshida
authors.affiliation: Faculty of Life and Medical Sciences, Doshisha University, Kyoto, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Watanabe
mesh_terms: D000327:Adsorption; D001710:Biotin / Q000737:chemistry*; D019921:Biotinylation / Q000379:methods; D007202:Indicators and Reagents / Q000737:chemistry*; D045423:Microbubbles; D058525:Quartz Crystal Microbalance Techniques / Q000379:methods*; D019809:Streptavidin / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D007202:Indicators and Reagents; D001710:Biotin; D019809:Streptavidin
keywords: Biotin-avidin bonds; Lipid shell; Quartz crystal microbalance; Resonant frequency; Targeting; Ultrasound contrast agent