title: Addition of poly (propylene glycol) to multiblock copolymer to optimize siRNA delivery.
abstract: Previous studies have examined different strategies for siRNA delivery with varying degrees of success. These include use of viral vectors, cationic liposomes, and polymers. Several copolymers were designed and synthesized based on blocks of poly(ethylene glycol) PEG, poly(propylene glycol) PPG, and poly(l-lysine). These were designated as P1, P2, and P3. We studied the copolymer self-assembly, siRNA binding, particle size, surface potential, architecture of the complexes, and siRNA delivery. Silencing of GFP using copolymer P3 to deliver GFP-specific siRNA to Neuro-2a cells expressing GFP was almost as effective as using Lipofectamine 2000, with minimal cytotoxicity. Thus, we have provided a new copolymer platform for siRNA delivery that we can continue to modify for improved delivery of siRNA in vitro and eventually in vivo.
authors.affiliation: Center of Excellence for Infectious Diseases; Department of Biomedical Sciences; Paul L Foster School of Medicine; Texas Tech University Health Sciences Center; El Paso, TX USA.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Dai
authors.affiliation: Center of Excellence for Infectious Diseases; Department of Biomedical Sciences; Paul L Foster School of Medicine; Texas Tech University Health Sciences Center; El Paso, TX USA.
authors.forename: Maria T
authors.initials: MT
authors.lastname: Arévalo
authors.affiliation: Center of Excellence for Infectious Diseases; Department of Biomedical Sciences; Paul L Foster School of Medicine; Texas Tech University Health Sciences Center; El Paso, TX USA.
authors.forename: Junwei
authors.initials: J
authors.lastname: Li
authors.affiliation: Center of Excellence for Infectious Diseases; Department of Biomedical Sciences; Paul L Foster School of Medicine; Texas Tech University Health Sciences Center; El Paso, TX USA.
authors.forename: Mingtao
authors.initials: M
authors.lastname: Zeng
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004337:Drug Carriers; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008823:Micelles; D008968:Molecular Conformation; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry; D011107:Polylysine / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011409:Propylene Glycols / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D055672:Static Electricity; D014170:Transformation, Genetic
chemical_list: D004337:Drug Carriers; D008823:Micelles; D011108:Polymers; D011409:Propylene Glycols; D034741:RNA, Small Interfering; D049452:Green Fluorescent Proteins; D011107:Polylysine; C012504:polypropylene glycol; D011092:Polyethylene Glycols
keywords: RNAi; copolymer; poly(L-lysine); poly(ethylene glycol); poly(propylene glycol); siRNA delivery