title: The evaluation and comparison of consecutive high speed centrifugation and LipoClear® reagent for lipemia removal.
abstract: BACKGROUND
  The aim of this study was to evaluate and compare the efficiency of high speed centrifugation and LipoClear® reagent for lipemia removal in plasma samples spiked with Intralipid®, for 26 biochemistry analytes.
  
  
  METHODS
  A plasma pool was collected. Aliquots of the pool were spiked with Intralipid® (final concentrations of 300mg/dL and 500mg/dL Intralipid®). The lipemia was removed from the aliquots by high speed centrifugation or LipoClear® reagent. 26 analytes were determined in native, lipemic plasma and in samples after lipemia removal. The bias from the concentration in the native sample was calculated for each parameter for Intralipid® concentrations, 300 and 500mg/dL of Intralipid®, respectively. Also, the recovery for each parameter after processing the samples using high speed centrifugation and LipoClear® was calculated. The biases and test recoveries were compared with the desirable specification for imprecision (DSI) according to Ricos available at the Wesgard's website. The bias and recovery for procalcitonin were compared with DSI according to Barassi and colleagues.
  
  
  RESULTS
  The bias of the spiked samples exceeded the DSI at 300mg/L Intralipid® for creatinine, glucose, total protein, iron and albumin; and for all previously mentioned parameters including CK-MB, sodium, potassium, chlorides, magnesium and ALP at concentration of 500mg/L Intralipid®. For the test recovery the DSI criteria were not met for calcium, total protein, sodium and chlorides after high speed centrifugation and for glucose, calcium, phosphates, magnesium, sodium, potassium, chlorides, ALP, GGT, CK-MB, total protein, albumin and troponin T after using LipoClear®.
  
  
  CONCLUSIONS
  LipoClear® is not suitable for lipemia removal from samples designated for glucose, sodium, potassium, chlorides, phosphates, magnesium, CK-MB, ALP, GGT, total protein, albumin, CRP and troponin T measurements. High speed centrifugation should be used for lipemia removal instead for glucose, potassium, phosphates, magnesium, CK-MB, ALP, GGT, albumin, CRP and TnT measurements.
authors.affiliation: University Department of Chemistry, University Hospital Centre "Sestre Milosrdnice", Zagreb, Croatia. Electronic address: andrea.saracevic@gmail.com.
authors.forename: Andrea
authors.initials: A
authors.lastname: Saracevic
authors.affiliation: University Department of Chemistry, University Hospital Centre "Sestre Milosrdnice", Zagreb, Croatia.
authors.forename: Nora
authors.initials: N
authors.lastname: Nikolac
authors.affiliation: University Department of Chemistry, University Hospital Centre "Sestre Milosrdnice", Zagreb, Croatia.
authors.forename: Ana-Maria
authors.initials: AM
authors.lastname: Simundic
mesh_terms: D016477:Artifacts; D001786:Blood Glucose / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis; D002116:Calcitonin / Q000097:blood; D015740:Calcitonin Gene-Related Peptide; D002118:Calcium / Q000097:blood; D002498:Centrifugation / Q000379:methods / Q000592:standards*; D003404:Creatinine / Q000097:blood; D004655:Emulsions / Q000302:isolation & purification; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood*; D007202:Indicators and Reagents / Q000592:standards; D008274:Magnesium / Q000097:blood; D010743:Phospholipids / Q000097:blood / Q000302:isolation & purification*; D011188:Potassium / Q000097:blood; D011498:Protein Precursors / Q000097:blood; D012964:Sodium / Q000097:blood; D013024:Soybean Oil / Q000097:blood / Q000302:isolation & purification*; D020107:Troponin T / Q000097:blood
chemical_list: D001786:Blood Glucose; D001798:Blood Proteins; C000606589:CALCA protein, human; D004655:Emulsions; D007202:Indicators and Reagents; D010743:Phospholipids; D011498:Protein Precursors; D020107:Troponin T; C545823:soybean oil, phospholipid emulsion; D013024:Soybean Oil; D002116:Calcitonin; D012964:Sodium; D003404:Creatinine; D008274:Magnesium; D015740:Calcitonin Gene-Related Peptide; D011188:Potassium; D002118:Calcium
keywords: Bias; Centrifugation; Interference; Lipemia; Quality improvement