title: Isolation and structural characterization of sugarcane bagasse lignin after dilute phosphoric acid plus steam explosion pretreatment and its effect on cellulose hydrolysis.
abstract: The structure of lignin after dilute phosphoric acid plus steam explosion pretreatment process of sugarcane bagasse in a pilot scale and the effect of the lignin extracted by ethanol on subsequent cellulose hydrolysis were investigated. The lignin structural changes caused by pretreatment were identified using advanced nondestructive techniques such as gel permeation chromatography (GPC), quantitative (13)C, and 2-D nuclear magnetic resonance (NMR). The structural analysis revealed that ethanol extractable lignin preserved basic lignin structure, but had relatively lower amount of β-O-4 linkages, syringyl/guaiacyl units ratio (S/G), p-coumarate/ferulate ratio, and other ending structures. The results also indicated that approximately 8% of mass weight was extracted by pure ethanol. The bagasse after ethanol extraction had an approximate 22% higher glucose yield after enzyme hydrolysis compared to pretreated bagasse without extraction.
authors.affiliation: Department of Agriculture & Biological Engineering, IFAS, University of Florida, Gainesville, FL 32611, USA.
authors.forename: Jijiao
authors.initials: J
authors.lastname: Zeng
authors.affiliation: Department of Agriculture & Biological Engineering, IFAS, University of Florida, Gainesville, FL 32611, USA. Electronic address: ztong@ufl.edu.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Tong
authors.affiliation: Department of Agriculture & Biological Engineering, IFAS, University of Florida, Gainesville, FL 32611, USA.
authors.forename: Letian
authors.initials: L
authors.lastname: Wang
authors.affiliation: USDA Forest Service, Forest Products Laboratory, One Gifford Pinchot Dr., Madison, WI 53726, USA.
authors.forename: J Y
authors.initials: JY
authors.lastname: Zhu
authors.affiliation: Department of Microbiology & Cell Science, University of Florida, PO Box 110700, Gainesville, FL 32606, USA.
authors.forename: Lonnie
authors.initials: L
authors.lastname: Ingram
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism*; D000431:Ethanol / Q000737:chemistry; D005947:Glucose / Q000096:biosynthesis; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry* / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D010756:Phosphoric Acids / Q000737:chemistry*; D031786:Saccharum / Q000737:chemistry*; D013227:Steam
chemical_list: D010756:Phosphoric Acids; D013227:Steam; D000431:Ethanol; D002482:Cellulose; D008031:Lignin; C027433:bagasse; C030242:phosphoric acid; D002480:Cellulase; D005947:Glucose
keywords: Cellulose hydrolysis; Lignin structure; Nuclear magnetic resonance (NMR); Soluble lignin