title: Effects of different oocyte retrieval and in vitro maturation systems on bovine embryo development and quality.
abstract: Cyclic adenosine monophosphate (cAMP) modulators have been used to avoid spontaneous oocyte maturation and concomitantly improve oocyte developmental competence. The current work evaluated the effects of the addition of cAMP modulators forskolin, 3-isobutyl-1-methylxanthine (IBMX) and cilostamide during in vitro maturation on the quality and yields of blastocysts. The following experimental groups were evaluated: (i) slicing or (ii) aspiration and maturation in tissue culture medium (TCM)199 for 24 h (TCM24slicing and TCM24aspiration, respectively), (iii) aspiration and maturation in the presence of cAMP modulators for 30 h (cAMP30aspiration) and in vivo-produced blastocysts. In vitro-matured oocytes were fertilized and presumptive zygotes were cultured in vitro to assess embryo development. Cleavage, blastocyst formation, blastocyst cell number, mRNA abundance of selected genes and global methylation profiles were evaluated. Blastocyst rate/zygotes for the TCM24aspiration protocol was improved (32.2 ± 2.1%) compared with TCM24slicing and cAMP30aspiration (23.4 ± 1.2% and 23.3 ± 2.0%, respectively, P 0.05), while those from the other groups were significantly elevated. It is concluded that retrieval, collection systems and addition of cAMP modulators can affect oocyte developmental competence, which is reflected not only in blastocyst rates but also in global DNA methylation and gene expression patterns.
authors.affiliation: Institute of Farm Animal Genetics,Biotechnology,Friedrich-Loeffler-Institut (FLI),Mariensee,Neustadt,Germany.
authors.forename: Sandra Milena Bernal
authors.initials: SM
authors.lastname: Ulloa
authors.affiliation: Institute of Farm Animal Genetics,Biotechnology,Friedrich-Loeffler-Institut (FLI),Mariensee,Neustadt,Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Heinzmann
authors.affiliation: Institute of Farm Animal Genetics,Biotechnology,Friedrich-Loeffler-Institut (FLI),Mariensee,Neustadt,Germany.
authors.forename: Doris
authors.initials: D
authors.lastname: Herrmann
authors.affiliation: Max Planck Institute for Molecular Genetics,Ihnestraße 63-73,14195,Berlin,Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Timmermann
authors.affiliation: Institute of Farm Animal Genetics,Biotechnology,Friedrich-Loeffler-Institut (FLI),Mariensee,Neustadt,Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Baulain
authors.affiliation: Minitube GmbH Hauptstrasse 41,Tiefenbach,Germany.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Großfeld
authors.affiliation: Weser-Ems-Union eG,Aussenstelle Rodenkirchen,Bullenmutterstation,Mittenfelder Weg 11,26935 Rodenkirchen,Germany.
authors.forename: Mike
authors.initials: M
authors.lastname: Diederich
authors.affiliation: Institute of Farm Animal Genetics,Biotechnology,Friedrich-Loeffler-Institut (FLI),Mariensee,Neustadt,Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Lucas-Hahn
authors.affiliation: Institute of Farm Animal Genetics,Biotechnology,Friedrich-Loeffler-Institut (FLI),Mariensee,Höltystraße 10,Mariensee,31535 Neustadt,Germany.
authors.forename: Heiner
authors.initials: H
authors.lastname: Niemann
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D002417:Cattle; D005576:Colforsin / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D019175:DNA Methylation; D005260:Female; D005307:Fertilization in Vitro; D018507:Gene Expression Regulation, Developmental; D059471:In Vitro Oocyte Maturation Techniques / Q000379:methods*; D008297:Male; D054315:Oocyte Retrieval / Q000379:methods*; D015363:Quinolones / Q000494:pharmacology
chemical_list: D003470:Culture Media; D015363:Quinolones; D005576:Colforsin; C021294:cilostamide; D015056:1-Methyl-3-isobutylxanthine
keywords: Preimplantation embryo