title: Bioactive lysophosphatidylcholine 16:0 and 18:0 are elevated in lungs of asthmatic subjects.
abstract: OBJECTIVE
  Asthma is a chronic inflammatory disease of the airways, and is associated with upregulation of phospholipase A2 (PLA2), the enzyme that hydrolyzes phosphatidylcholine, producing lysophosphatidylcholine (LPC) and free fatty acids. LPC is a lipid mediator with known pro-inflammatory and pro-atherogenic properties, and is believed to be a critical factor in cardiovascular diseases. We postulate that asthmatic subjects have an elevated content of LPC in the lung lining fluids.
  
  
  METHODS
  Eight non-asthmatic controls and seven asthmatic subjects were recruited for broncho-alveolar lavage fluids (BALF) collection for analysis of LPC by high performance liquid chromatography-tandem mass spectrometry.
  
  
  RESULTS
  LPC16:0 and LPC18:0 were significantly elevated in the BALF of asthmatics with impaired lung function characteristic of moderate asthma, but not mild asthma. The increased LPC content in BALF was accompanied by increased PLA2 activity. Furthermore, qRT-PCR analysis of the BALF cell fraction indicated increased secretory PLA2-X (sPLA2-X).
  
  
  CONCLUSIONS
  The increased LPC content in the lung lining fluids is a potential critical lipid mediator in the initiation and/or progression of airway epithelial injury in asthma.
authors.affiliation: Department of Medicine, Rush University Medical Center, Chicago, IL, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Yoder
authors.affiliation: Department of Pharmacology, Rush University Medical Center, Chicago, IL, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhuge
authors.affiliation: Department of Medicinal Chemistry & Pharmacognosy, University of Illinois, Chicago, IL, USA.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Department of Pharmacology, Rush University Medical Center, Chicago, IL, USA.
authors.forename: Oksana
authors.initials: O
authors.lastname: Holian
authors.affiliation: Department of Medicine, Rush University Medical Center, Chicago, IL, USA.
authors.forename: Sherry
authors.initials: S
authors.lastname: Kuo
authors.affiliation: Department of Medicinal Chemistry & Pharmacognosy, University of Illinois, Chicago, IL, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: van Breemen
authors.affiliation: Department of Immunology and Microbiology, Rush University Medical Center, Chicago, IL, USA.
authors.forename: Larry L
authors.initials: LL
authors.lastname: Thomas
authors.affiliation: Department of Pharmacology, Rush University Medical Center, Chicago, IL, USA.
authors.forename: Hazel
authors.initials: H
authors.lastname: Lum
keywords: Lysophosphatidylcholine; asthma; phospholipase A2