title: [Effect of Chinese herbal compounds for blood activating stasis removing, qi benefiting Shen invigorating on high glucose stimulated proliferation of renal mesangial cells and expressions of fibronectin].
abstract: OBJECTIVE
  To study effects of Chinese Herbal Compounds (CHC) for blood activating stasis removing (BASR), qi benefiting Shen invigorating (QBSI) on high glucose stimulated proliferation of renal mesangial cells (RMCs) and expressions of fibronectin (FN).
  
  
  METHODS
  Rats' RMCs were dealt with high glucose and different concentrations of Chinese medicine for 24 and 48 h respectively. The proliferation of RMCs was detected with 4-A thiazolyl blue. mRNA expressions of FN was detected by real time quantitative PCR. The protein expression of FN was detected by ELISA.
  
  
  RESULTS
  Compared with the control group, the proliferation obviously increased (P < 0.05, P < 0.01) after 24 and 48 h of treatment in the high glucose group, mRNA and protein expressions of FN also increased (P < 0.01). There was no statistical difference in the proliferation of RMCs or expressions of FN at 24 h between each CHC group and the high glucose group (P > 0.05). Compared with the high glucose group, the proliferation of RMCs and expressions of FN at 24 h each obviously decreased in the CHC group (P < 0.05, P < 0.01).
  
  
  CONCLUSIONS
  High glucose could promote the proliferation of RMCs and induce expressions of FN. No obvious effect could be stimulated by CHC treatment for 24 h. The proliferation of RMCs, protein and mRNA expressions of FN could be reversed by CHC treatment for 48 h.
authors.affiliation: Department of Endocrinology, Provincial Hospital Affiliated to Shandong University, Institute of Endocrinology and Metabolism, Shandong Clinical Medicine Academy, Jinan 250021, China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Liu
authors.affiliation: Department of Endocrinology, Provincial Hospital Affiliated to Shandong University, Institute of Endocrinology and Metabolism, Shandong Clinical Medicine Academy, Jinan 250021, China.
authors.forename: Wen-Xia
authors.initials: WX
authors.lastname: Han
authors.affiliation: Department of Anesthesia, West Ward of Shandong Provincial Hospital, Jinan 250022, China.
authors.forename: Yu-Wen
authors.initials: YW
authors.lastname: Song
authors.affiliation: Department of Endocrinology, Provincial Hospital Affiliated to Shandong University, Institute of Endocrinology and Metabolism, Shandong Clinical Medicine Academy, Jinan 250021, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Lou
authors.affiliation: Department of Endocrinology, Provincial Hospital Affiliated to Shandong University, Institute of Endocrinology and Metabolism, Shandong Clinical Medicine Academy, Jinan 250021, China.
authors.forename: Chun-Xiao
authors.initials: CX
authors.lastname: Yu
authors.affiliation: Central Laboratory, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Gao
authors.affiliation: Department of Endocrinology, Provincial Hospital Affiliated to Shandong University, Institute of Endocrinology and Metabolism, Shandong Clinical Medicine Academy, Jinan 250021, China.
authors.forename: Jia-Jun
authors.initials: JJ
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005353:Fibronectins / Q000378:metabolism*; D005947:Glucose / Q000009:adverse effects; D007684:Kidney Tubules / Q000166:cytology; D008297:Male; D050527:Mesangial Cells / Q000187:drug effects* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats
chemical_list: D004365:Drugs, Chinese Herbal; D005353:Fibronectins; D012333:RNA, Messenger; D005947:Glucose