title: Effect of combination of dietary fish protein and fish oil on lipid metabolism in rats.
abstract: This study examined the effects of fish protein in combination with fish oil on rat lipid metabolism. Male Wistar rats were divided into four groups and fed an AIN93G-based diet with casein (20%) + soybean oil (7%), casein (10%) + fish protein (10%) + soybean oil (7%), casein (20%) + soybean oil (5%) + fish oil (2%), and casein (10%) + fish protein (10%) + soybean oil (5%) + fish oil (2%) for 4 weeks. The dietary combination of fish protein and fish oil decreased the contents of serum triacylglycerol, serum cholesterol, liver triacylglycerol and liver cholesterol in addition to altering liver lipid fatty acid composition. These effects are partly due to the increase in fecal cholesterol, bile acid excretion, and increased enzyme activities of fatty acid β-oxidation in the liver. These data suggest that combined intake of fish protein and fish oil lead to both hypocholesterolemic and hypotriglyceridemic in serum and the liver, while sole intake of fish protein or fish oil decrease only cholesterol and triglyceride levels, respectively. These results suggest that combined intake of fish protein and fish oil may play beneficial roles in the prevention of lifestyle-related diseases as compared with sole fish protein intake.
authors.affiliation: Department of Life Science and Biotechnology, Faculty of Chemistry, Materials and Bioengineering, Kansai University, Suita, Osaka 564-8680 Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Hosomi
authors.affiliation: Department of Life Science and Biotechnology, Faculty of Chemistry, Materials and Bioengineering, Kansai University, Suita, Osaka 564-8680 Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Fukunaga
authors.affiliation: Division of Biotechnology and Environmental Chemistry, Kitami Institute of Technology, Kitami, Hokkaido 090-8507 Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Arai
authors.affiliation: Department of Public Health, Kansai Medical University, Moriguchi, Osaka 570-8506 Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Kanda
authors.affiliation: Department of Public Health, Kansai Medical University, Moriguchi, Osaka 570-8506 Japan.
authors.forename: Toshimasa
authors.initials: T
authors.lastname: Nishiyama
authors.affiliation: Department of Life Science and Biotechnology, Faculty of Chemistry, Materials and Bioengineering, Kansai University, Suita, Osaka 564-8680 Japan.
authors.forename: Munehiro
authors.initials: M
authors.lastname: Yoshida
keywords: Fish oil; Fish protein; Lipid metabolism; n-3 polyunsaturated fatty acid