title: Characterization of laminaran and a highly sulfated polysaccharide from Sargassum fusiforme.
abstract: The crude polysaccharide (HFS) from Sargassum fusiforme (Hizikia fusiforme) was extracted using 0.1M HCl and was fractionated by anion-exchange chromatography into three fractions: HFS-1, HFS-2, and HFS-3. Based on the chemical analysis, HFS-1 was composed of laminaran, HFS-2 was a mixture of alginate and sulfated heteropolysaccharides, and HFS-3 was primarily composed of sulfated galactofucan. The NMR spectra revealed that HFS-1 was composed of a soluble laminaran with chains that are terminated by β-d-glucose residues. In contrast, the spectra obtained for HFS-2 were still complex, even after most of the alginate was removed. In addition, HFS-3 might contain 3-linked fucan sulfated at C-2, 6-linked galactan sulfated at C-2 and branched at C-4 by 2-sulfated Fuc, and galactofucan with a backbone of either alternating Gal and Fuc sulfated at C-2 or alternating (Gal)n and (Fuc)n sulfated at C-2. Moreover, HFS-3 also contained small amounts of fucoglucuronomannan and xylan.
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China; University of the Chinese Academy of Sciences, Beijing 100049, PR China; Nantong Branch, Institute of Oceanology, Chinese Academy of Sciences, Jiangsu 226006, PR China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Jin
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China; University of the Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China; Nantong Branch, Institute of Oceanology, Chinese Academy of Sciences, Jiangsu 226006, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Medicine and Pharmaceutics, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Sumei
authors.initials: S
authors.lastname: Ren
authors.affiliation: College of Medicine and Pharmaceutics, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Ni
authors.initials: N
authors.lastname: Song
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China.
authors.forename: Delin
authors.initials: D
authors.lastname: Duan
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China; Nantong Branch, Institute of Oceanology, Chinese Academy of Sciences, Jiangsu 226006, PR China. Electronic address: qbzhang@ms.qdio.ac.cn.
authors.forename: Quanbin
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000464:Alginates / Q000737:chemistry; D002240:Carbohydrate Sequence; D005936:Glucans; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D044647:Sargassum / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013463:Sulfuric Acid Esters / Q000737:chemistry*
chemical_list: D000464:Alginates; D005936:Glucans; D006603:Hexuronic Acids; D011134:Polysaccharides; D013463:Sulfuric Acid Esters; D020723:Glucuronic Acid; C008247:laminaran
keywords: ESI-CID-MS/MS; Laminaran; NMR; Sargassum fusiforme; Sulfated polysaccharide