title: Quantification of trehalose in biological samples with a conidial trehalase from the thermophilic fungus Humicola grisea var. thermoidea.
abstract: The partially-purified, thermally-stable trehalase from conidia of Humicola grisea was highly specific for trehalose and was free of potentially interfering activities. The enzyme was fully stable when stored in solution at -15°C for at least 6 months. This preparation could be used to quantify trehalose from 0.05 to 1.25 μmol/ml either in carbohydrate mixtures or in complex biological materials.
authors.affiliation: , .
authors.forename: M J
authors.initials: MJ
authors.lastname: Neves
authors.forename: H F
authors.initials: HF
authors.lastname: Terenzi
authors.forename: F A
authors.initials: FA
authors.lastname: Leone
authors.forename: J A
authors.initials: JA
authors.lastname: Jorge