title: Shear reversible cell/microsphere aggregate as an injectable for tissue regeneration.
abstract: Injectable delivery systems have been widely used in tissue engineering as they can deliver cells into the body in a minimally invasive manner. In this study, it is hypothesized that microspheres with a similar size of cells could effectively form a shear reversible aggregate in the presence of cells and the aggregate could be useful to engineer tissues. Alginate microspheres are prepared by an emulsion method, followed by modification with a peptide containing the arginine-glycine-aspartic acid (RGD) sequence. RGD-modified alginate microspheres form an aggregate in the presence of chondrocytes, and the aggregation behavior is shear reversible. This cell/microsphere aggregate is useful to deliver chondrocytes into an animal model using a syringe, and effectively regenerates cartilage tissues in vivo.
authors.affiliation: Department of Bioengineering, Hanyang University, Seoul, 133-791, Republic of Korea.
authors.forename: Eunkyeong
authors.initials: E
authors.lastname: Woo
authors.forename: Honghyun
authors.initials: H
authors.lastname: Park
authors.forename: Kuen Yong
authors.initials: KY
authors.lastname: Lee
mesh_terms: D000464:Alginates / Q000737:chemistry; D000818:Animals; D002356:Cartilage / Q000502:physiology; D019902:Chondrocytes / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D016503:Drug Delivery Systems / Q000379:methods*; D006025:Glycosaminoglycans / Q000032:analysis; D048091:Guided Tissue Regeneration / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D009842:Oligopeptides / Q000737:chemistry; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012212:Rheology; D033081:Shear Strength; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D000464:Alginates; D017931:DNA Primers; D006025:Glycosaminoglycans; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid
keywords: aggregate; alginate; cartilage regeneration; microsphere; tissue engineering