title: Criteria for detection of alpha-thalassemia-1 Thai type deletion in routine laboratory.
abstract: BACKGROUND
  Using a single tube multiplex real-time PCR with SYBR Green1 and high resolution melting (HRM) analysis in routine laboratory for detection of alpha-thalassemia-1 Southeast Asian (SEA) type deletion simultaneously with Thai type deletion is costly.
  
  
  METHODS
  Criteria for laboratory testing of alpha-thalassemia-1 Thai type deletion were set as follow: (1) MCV < or = 75 fL and negative for alpha-thalassemia-1 SEA type deletion, beta-thalassemia or homozygous HbE; (2) HbE trait with HbE level < 21.5% and negative for a-thalassemia-1 SEA type deletion; or (3) Hb Bart's and HbH found on HPLC chromatogram but negative for alpha-thalassemia-1 SEA type deletion.
  
  
  RESULTS
  From May 2010 to October 2012, the number of samples to detect for alpha-thalassemia-1 Thai type deletion decreased from 15,081 to 672 samples and the alpha-thalassemia-1 Thai type deletion trait was found in 7 samples (1.04%).
  
  
  CONCLUSIONS
  The new testing algorithm can reduce number of samples for detection of alpha-thalassemia-1 Thai type deletion.
authors.affiliation: Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang-Mai University, Chiang-Mai, Thailand. sakornmi001@gmail.com
authors.forename: Sakorn
authors.initials: S
authors.lastname: Pornprasert
authors.affiliation: Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang-Mai University, Chiang-Mai, Thailand.
authors.forename: Manoo
authors.initials: M
authors.lastname: Punyamung
authors.affiliation: Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang-Mai University, Chiang-Mai, Thailand.
authors.forename: Kallayanee
authors.initials: K
authors.lastname: Treesuwan
mesh_terms: D019411:Clinical Laboratory Techniques; D006801:Humans; D007753:Laboratories; D017384:Sequence Deletion; D013785:Thailand; D017085:alpha-Thalassemia / Q000175:diagnosis* / Q000235:genetics