title: Superparamagnetic iron oxide nanoparticles conjugated with epidermal growth factor (SPION-EGF) for targeting brain tumors.
abstract: Superparamagnetic iron oxide nanoparticles (SPIONs) conjugated with recombinant human epidermal growth factor (SPION-EGF) were studied as a potential agent for magnetic resonance imaging contrast enhancement of malignant brain tumors. Synthesized conjugates were characterized by transmission electron microscopy, dynamic light scattering, and nuclear magnetic resonance relaxometry. The interaction of SPION-EGF conjugates with cells was analyzed in a C6 glioma cell culture. The distribution of the nanoparticles and their accumulation in tumors were assessed by magnetic resonance imaging in an orthotopic model of C6 gliomas. SPION-EGF nanosuspensions had the properties of a negative contrast agent with high coefficients of relaxation efficiency. In vitro studies of SPION-EGF nanoparticles showed high intracellular incorporation and the absence of a toxic influence on C6 cell viability and proliferation. Intravenous administration of SPION-EGF conjugates in animals provided receptor-mediated targeted delivery across the blood-brain barrier and tumor retention of the nanoparticles; this was more efficient than with unconjugated SPIONs. The accumulation of conjugates in the glioma was revealed as hypotensive zones on T2-weighted images with a twofold reduction in T2 relaxation time in comparison to unconjugated SPIONs (P<0.001). SPION-EGF conjugates provide targeted delivery and efficient magnetic resonance contrast enhancement of EGFR-overexpressing C6 gliomas.
authors.affiliation: Institute of Cytology of the Russian Academy of Sciences (RAS), St Petersburg, Russia ; AL Polenov Russian Scientific Research Institute of Neurosurgery, St Petersburg, Russia.
authors.forename: Maxim A
authors.initials: MA
authors.lastname: Shevtsov
authors.affiliation: Research Institute of Highly Pure Biopreparations, St Petersburg, Russia.
authors.forename: Boris P
authors.initials: BP
authors.lastname: Nikolaev
authors.affiliation: Research Institute of Highly Pure Biopreparations, St Petersburg, Russia.
authors.forename: Ludmila Y
authors.initials: LY
authors.lastname: Yakovleva
authors.affiliation: Research Institute of Highly Pure Biopreparations, St Petersburg, Russia.
authors.forename: Yaroslav Y
authors.initials: YY
authors.lastname: Marchenko
authors.affiliation: Institute of Macromolecular Compounds of the Russian Academy of Sciences (RAS), St Petersburg, Russia.
authors.forename: Anatolii V
authors.initials: AV
authors.lastname: Dobrodumov
authors.affiliation: IM Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences (RAS), St Petersburg, Russia.
authors.forename: Anastasiya L
authors.initials: AL
authors.lastname: Mikhrina
authors.affiliation: Institute of Cytology of the Russian Academy of Sciences (RAS), St Petersburg, Russia.
authors.forename: Marina G
authors.initials: MG
authors.lastname: Martynova
authors.affiliation: Institute of Cytology of the Russian Academy of Sciences (RAS), St Petersburg, Russia.
authors.forename: Olga A
authors.initials: OA
authors.lastname: Bystrova
authors.affiliation: AL Polenov Russian Scientific Research Institute of Neurosurgery, St Petersburg, Russia.
authors.forename: Igor V
authors.initials: IV
authors.lastname: Yakovenko
authors.affiliation: Research Institute of Highly Pure Biopreparations, St Petersburg, Russia.
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Ischenko
mesh_terms: D000818:Animals; D017209:Apoptosis; D001932:Brain Neoplasms / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival; D003911:Dextrans / Q000008:administration & dosage* / Q000737:chemistry* / Q000648:ultrastructure; D004815:Epidermal Growth Factor / Q000737:chemistry / Q000235:genetics / Q000493:pharmacokinetics*; D005910:Glioma / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D058185:Magnetite Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry* / Q000648:ultrastructure; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry / Q000648:ultrastructure; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000493:pharmacokinetics; D016896:Treatment Outcome
chemical_list: D003911:Dextrans; D058185:Magnetite Nanoparticles; D053769:Nanocapsules; D011994:Recombinant Proteins; C097921:ferumoxtran-10; D004815:Epidermal Growth Factor
keywords: C6 glioma; EGFR; MRI contrast agent; SPION; brain tumor; epidermal growth factor; magnetic nanoparticles