title: [Expression and significance of FGFR2IIIc in chemoresistant bladder cancer cells].
abstract: OBJECTIVE
  To investigate the role of fibroblast growth factor receptor-2 (FGFR2) splice variant FGFR2IIIc in the regulation of mesenchymal-epithelial transition (MET) in doxorubicin-resistant bladder cancer cells.
  
  
  METHODS
  A doxorubicin-resistant human bladder cell line (253J/DOX) was generated from the bladder cancer cell line 253J by being continuously exposed to gradually increasing doses of doxorubicin. Chemosensitivity to doxorubicin was determined by MTT assay. The expressions of P-glycoprotein and FGFR2IIIc were evaluated by Western blotting and real-time RT-PCR, respectively. Changes in E-cadherin and vimentin were detected by Western blot analysis. Migration ability of 253J and 253J/DOX cells was analyzed by in vitro wound healing assay.
  
  
  RESULTS
  The resistant cells, 253J/DOX, were more resistant to doxorubicin than the parent cells. Western blotting and RT-PCR analysis indicated the higher levels of P-glycoprotein and FGFR2IIIc in 253J/DOX cells (P<0.05). Additionally, compared with the 253J cells, 253J/DOX cells presented the upregulation of E-cadherin, the downregulation of vimentin and the inhibition of migration ability.
  
  
  CONCLUSIONS
  FGFR2IIIc-induced MET in chemoresistant bladder cancer cells may play an important role in the formation of metastatic lesions.
authors.affiliation: Department of Urology, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Yule
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Urology, First Affiliated Hospital, Department of Oncology Research, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Urology, First Affiliated Hospital, Department of Oncology Research, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Kaijie
authors.initials: K
authors.lastname: Wu
authors.affiliation: Department of Urology, First Affiliated Hospital, Department of Oncology Research, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zeng
authors.affiliation: Department of Urology, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Oncology Research, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Xinyang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pathology, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Honghong
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Urology, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Luke S
authors.initials: LS
authors.lastname: Chang
authors.affiliation: Department of Urology, First Affiliated Hospital, Department of Oncology Research, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Dalin
authors.initials: D
authors.lastname: He
mesh_terms: D000818:Animals; D054338:Cell Transdifferentiation / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D006801:Humans; D009362:Neoplasm Metastasis; D020033:Protein Isoforms / Q000378:metabolism; D051497:Receptor, Fibroblast Growth Factor, Type 2 / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000473:pathology*
chemical_list: D020033:Protein Isoforms; D004317:Doxorubicin; D051497:Receptor, Fibroblast Growth Factor, Type 2