title: Photodynamic effect of hypericin after topical application in the ex ovo quail chorioallantoic membrane model.
abstract: Photosensitizing properties of hypericin are well known, and the chicken chorioallantoic membrane has previously been used to test photodynamic effects of hypericin and other substances. In our study the photodynamic effect of hypericin in the ex ovo quail chorioallantoic membrane model was evaluated. Steady-state and time-resolved fluorescence spectroscopy of hypericin solution in PEG-400 and its mixture in PBS was performed to assess and characterize the process of aggregation and disaggregation of hypericin during the drug formulation preparation. A therapeutical formulation (2 µg/g of embryo weight) was topically applied on CAM into the silicone ring. Hypericin was excited by diode laser with wavelength 405 nm, fluence rate 140 mW/cm2, and fluence 16.8 J/cm2. Hypericin in 100% PEG-400 exhibits typical fluorescence spectra with a maximum of about 600 nm, while hypericin 10% PEG-400 formulation exhibits almost no fluorescence. Time resolved spectra analysis showed fluorescence decay of hypericin in 100% PEG-400 solution with a mean lifetime of 5.1 ns and in 10% PEG 4.1 ns. Damage of quail chorioallantoic membrane vasculature after photodynamic therapy ranged from hemorrhage and vanishing of capillary vessels to thrombosis, lysis, and hemorrhage of larger vessels.The presented findings suggest that quail embryos can be used as a suitable model to test the effect of hypericin and other photodynamic compounds.
authors.affiliation: Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovakia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Čavarga
authors.affiliation: Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovakia.
authors.forename: Boris
authors.initials: B
authors.lastname: Bilčík
authors.affiliation: Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovakia.
authors.forename: Pavel
authors.initials: P
authors.lastname: Výboh
authors.affiliation: Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovakia.
authors.forename: Monika
authors.initials: M
authors.lastname: Záškvarová
authors.affiliation: International Laser Centre, Bratislava, Slovakia.
authors.forename: Dušan
authors.initials: D
authors.lastname: Chorvát
authors.affiliation: Polymer Institute, Slovak Academy of Sciences, Bratislava, Slovakia.
authors.forename: Peter
authors.initials: P
authors.lastname: Kasák
authors.affiliation: St Elizabeth Oncological Institute, Bratislava, Slovakia.
authors.forename: Peter
authors.initials: P
authors.lastname: Mlkvý
authors.affiliation: International Laser Centre, Bratislava, Slovakia.
authors.forename: Anton
authors.initials: A
authors.lastname: Mateašík
authors.affiliation: International Laser Centre, Bratislava, Slovakia.
authors.forename: Alžbeta
authors.initials: A
authors.lastname: Chorvátová
authors.affiliation: Dept. of Biophysics, Faculty of Sciences, P. J. Šafárik University, Košice, Slovakia.
authors.forename: Pavol
authors.initials: P
authors.lastname: Miškovský
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000873:Anthracenes; D001808:Blood Vessels / Q000187:drug effects; D049033:Chorioallantoic Membrane / Q000098:blood supply* / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005260:Female; D054023:Lasers, Semiconductor; D010569:Perylene / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D010778:Photochemotherapy / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry / Q000633:toxicity; D011784:Quail; D013050:Spectrometry, Fluorescence
chemical_list: D000873:Anthracenes; D011092:Polyethylene Glycols; D010569:Perylene; C004965:hypericin