title: Serological diagnosis of dengue infection in blood plasma using long-range surface plasmon waveguides.
abstract: We present a compact, cost-effective, label-free, real-time biosensor based on long-range surface plasmon polariton (LRSPP) gold (Au) waveguides for the detection of dengue-specific immunoglobulin M (IgM) antibody, and we demonstrate detection in actual patient blood plasma samples. Two surface functionalization approaches are proposed and demonstrated: a dengue virus serotype 2 (DENV-2) functionalized surface to capture dengue-specific IgM antibody in blood plasma and the reverse, a blood plasma functionalized surface to capture DENV-2. The results obtained via these two surface functionalization approaches are comparable to, or of greater quality, than those collected by conventional IgM antibody capture enzyme linked immunosorbent assay (MAC-ELISA). Our second functionalization approach was found to minimize nonspecific binding, thus improving the sensitivity and accuracy of the test. We also demonstrate reuse of the biosensors by regenerating the sensing surface down to the virus (or antibody) level or down to the bare Au.
authors.affiliation: Photonics Research Group, Department of Electrical Engineering, Faculty of Engineering, University of Malaya , 50603 Kuala Lumpur, Malaysia.
authors.forename: Wei Ru
authors.initials: WR
authors.lastname: Wong
authors.forename: Oleksiy
authors.initials: O
authors.lastname: Krupin
authors.forename: Shamala Devi
authors.initials: SD
authors.lastname: Sekaran
authors.forename: Faisal Rafiq
authors.initials: FR
authors.lastname: Mahamd Adikan
authors.forename: Pierre
authors.initials: P
authors.lastname: Berini
mesh_terms: D015374:Biosensing Techniques / Q000191:economics / Q000379:methods*; D003362:Cost-Benefit Analysis; D003715:Dengue / Q000097:blood*; D003716:Dengue Virus / Q000502:physiology*; D006046:Gold / Q000737:chemistry; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D012698:Serologic Tests / Q000191:economics / Q000379:methods*; D013499:Surface Properties
chemical_list: D007075:Immunoglobulin M; D006046:Gold