title: Short communication: Purification and properties of a glucose-forming amylase of Lactobacillus brevis.
abstract: An extracellular glucose-forming amylase was produced by Lactobacillus brevis isolated from 'Kagasok tea'. The enzyme was purified 70-fold and had optimal activity at 55°C and pH 6.5. Its K m value for starch was 0.27 mg ml(-1) and its M r was approx. 75,900 Da. The activity of the enzyme was enhanced by Ca(2+), Mg(2+), Na(+) or K(+) and inhibited by EDTA, KCN, citric acid and L-cysteine.
authors.affiliation: Department of Botany and Microbiology, Lagos State University, P.M.B. 1087, Apapa, Lagos, Nigeria.
authors.forename: M O
authors.initials: MO
authors.lastname: Ilori
authors.forename: O O
authors.initials: OO
authors.lastname: Amund
authors.forename: O
authors.initials: O
authors.lastname: Omidiji