title: Temperature-sensitive, lipopolysaccharide-deficient mutants of Salmonella typhimurium.
abstract: Two mutants of Salmonella typhimurium LT2, which were temperature-sensitive for lipopolysaccharide (LPS) synthesis, were isolated from a galE (-) strain based on their resistance to phage C21 and sensitivity to sodium deoxycholate at 42°C. They produced LPS of chemotype Rc at 30°C and deep-rough LPS at 42°C. P22-mediated transductional analysis showed that the mutations responsible for temperature sensitivity are located in the rfa cluster where several genes involved in the synthesis of the LPS core are mapped. A plasmid, carrying rfaC, D and F genes of Escherichia coli K-12, complemented these mutations. These genes are responsible for the synthesis of the inner-core region of the LPS molecule. This indicates that genetic defects in these temperature-sensitive mutants affect the inner-core region of LPS.
authors.affiliation: Salmonella Genetic Stock Centre, Department of Biological Sciences, University of Calgary, T2N 1N4, Calgary, Alberta, Canada.
authors.forename: D M
authors.initials: DM
authors.lastname: Sirisena
authors.forename: K E
authors.initials: KE
authors.lastname: Sanderson