title: Simple method of DNA stretching on glass substrate for fluorescence imaging and spectroscopy.
abstract: We demonstrate a simple method of stretching DNA to its full length, suitable for optical imaging and atomic force microscopy (AFM). Two competing forces on the DNA molecules, which are the electrostatic attraction between positively charged dye molecules (YOYO-1) intercalated into DNA and the negatively charged surface of glass substrate, and the centrifugal force of the rotating substrate, are mainly responsible for the effective stretching and the dispersion of single strands of DNA. The density of stretched DNA molecules could be controlled by the concentration of the dye-stained DNA solution. Stretching of single DNA molecules was confirmed by AFM imaging and the photoluminescence spectra of single DNA molecule stained with YOYO-1 were obtained, suggesting that our method is useful for spectroscopic analysis of DNA at the single molecule level.
authors.affiliation: IBS Center for Integrated Nanostructure Physics, Institute for Basic Science (IBS), Daejeon 305-701, Republic of KoreabSungkyunkwan University, Department of Energy Science, Suwon 440-746, Republic of Korea.
authors.forename: Guru P
authors.initials: GP
authors.lastname: Neupane
authors.affiliation: IBS Center for Integrated Nanostructure Physics, Institute for Basic Science (IBS), Daejeon 305-701, Republic of KoreabSungkyunkwan University, Department of Energy Science, Suwon 440-746, Republic of Korea.
authors.forename: Krishna P
authors.initials: KP
authors.lastname: Dhakal
authors.affiliation: IBS Center for Integrated Nanostructure Physics, Institute for Basic Science (IBS), Daejeon 305-701, Republic of Korea.
authors.forename: Min Su
authors.initials: MS
authors.lastname: Kim
authors.affiliation: Wake Forest University, Department of Physics, Winston-Salem, North Carolina 27109.
authors.forename: Hyunsoo
authors.initials: H
authors.lastname: Lee
authors.affiliation: Wake Forest University, Department of Physics, Winston-Salem, North Carolina 27109.
authors.forename: Martin
authors.initials: M
authors.lastname: Guthold
authors.affiliation: Incheon National University, Department of Chemistry, Incheon 406-772, Republic of Korea.
authors.forename: Vincent S
authors.initials: VS
authors.lastname: Joseph
authors.affiliation: Incheon National University, Department of Chemistry, Incheon 406-772, Republic of Korea.
authors.forename: Jong-Dal
authors.initials: JD
authors.lastname: Hong
authors.affiliation: IBS Center for Integrated Nanostructure Physics, Institute for Basic Science (IBS), Daejeon 305-701, Republic of KoreabSungkyunkwan University, Department of Energy Science, Suwon 440-746, Republic of KoreaeIncheon National University, Department of Physi.
authors.forename: Jeongyong
authors.initials: J
authors.lastname: Kim
mesh_terms: D001583:Benzoxazoles / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D005898:Glass; D006801:Humans; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D057054:Molecular Imaging / Q000295:instrumentation / Q000379:methods; D010920:Placenta / Q000737:chemistry; D011247:Pregnancy; D011806:Quinolinium Compounds / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*
chemical_list: D001583:Benzoxazoles; D005456:Fluorescent Dyes; D011806:Quinolinium Compounds; C075296:1,1'-((4,4,7,7-tetramethyl)-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-oxazole)-2-methylidene)quinolinium; D004247:DNA