title: Preparative separation of polyphenols from the flowers of Paeonia lactiflora Pall. by high-speed counter-current chromatography.
abstract: High-speed counter-current chromatography was used to separate and purify polyphenols for the first time from the flowers of Paeonia lactiflora Pall. with two solvent systems as follows: petroleum ether-ethyl acetate-H2O (1:9:10, v/v/v/v) and petroleum ether-ethyl acetate-butanol-H2O (1:9:0.5:10, v/v/v/v). Eight compounds were separated successfully in single run which were identified as quercetin-3-O-(6″-O-galloyl)-glucoside (I, 41.5 mg), 1,2,3,4,6-trigalloyl-β-D-glucose (II, 106.2mg), quercetin-3-O-β-D-glucoside (III, 42.3 mg), kaempferol-3-O-(6″-O-galloyl)-glucoside (IV, 23.5 mg), isohamnetin-3-O-β-D-glucoside (V, 34.1 mg), kaempferol (VI, 14.8 mg), kaempferol-3-O-β-D-glucoside (VII, 32.6 mg), kaempferol-7-O-β-D-glucoside (VIII, 23.8 mg) by electrospray ionization-mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR). The purities of compounds I-VIII were all over 97.0% as determined by HPLC.
authors.affiliation: Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, Shandong 250014, China.
authors.forename: Xikai
authors.initials: X
authors.lastname: Shu
authors.affiliation: Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, Shandong 250014, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Duan
authors.affiliation: Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, Shandong 250014, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.affiliation: Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, Shandong 250014, China.
authors.forename: Xingang
authors.initials: X
authors.lastname: Shi
authors.affiliation: Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, Shandong 250014, China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Geng
authors.affiliation: Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, Shandong 250014, China. Electronic address: wangx@sdas.org.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: Sanfeng Biological Engineering Technology Co., 203 Beiyuan Street, Jinan, Shandong 250014, China.
authors.forename: Bingtian
authors.initials: B
authors.lastname: Yang
mesh_terms: D003377:Countercurrent Distribution / Q000379:methods*; D035264:Flowers / Q000737:chemistry; D029596:Paeonia / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification*; D059808:Polyphenols / Q000032:analysis / Q000302:isolation & purification*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D010936:Plant Extracts; D059808:Polyphenols
keywords: EtOAc; H(2)O; HPLC; HSCCC; High-speed counter-current chromatography; MeOH; PTFE; Paeonia lactiflora Pall.; Pet; Separation; ethyl acetate; high-performance liquid chromatography; high-speed counter-current chromatography; methanol; petroleum ether; polytetrafluoroethylene; water