title: Development and evaluation of a blocking enzyme-linked immunosorbent assay and virus neutralization assay to detect antibodies to viral hemorrhagic septicemia virus.
abstract: Viral hemorrhagic septicemia virus (VHSV) is a target of surveillance by many state and federal agencies in the United States. Currently, the detection of VHSV relies on virus isolation, which is lethal to fish and indicates only the current infection status. A serological method is required to ascertain prior exposure. Here, we report two serologic tests for VHSV that are nonlethal, rapid, and species independent, a virus neutralization (VN) assay and a blocking enzyme-linked immunosorbent assay (ELISA). The results show that the VN assay had a specificity of 100% and sensitivity of 42.9%; the anti-nucleocapsid-blocking ELISA detected nonneutralizing VHSV antibodies at a specificity of 88.2% and a sensitivity of 96.4%. The VN assay and ELISA are valuable tools for assessing exposure to VHSV.
authors.affiliation: Department of Pathobiological Sciences, University of Wisconsin, Madison, Wisconsin, USA.
authors.forename: Anna
authors.initials: A
authors.lastname: Wilson
authors.forename: Tony
authors.initials: T
authors.lastname: Goldberg
authors.forename: Susan
authors.initials: S
authors.lastname: Marcquenski
authors.forename: Wendy
authors.initials: W
authors.lastname: Olson
authors.forename: Frederick
authors.initials: F
authors.lastname: Goetz
authors.forename: Paul
authors.initials: P
authors.lastname: Hershberger
authors.forename: Lucas
authors.initials: L
authors.lastname: Hart
authors.forename: Kathy
authors.initials: K
authors.lastname: Toohey-Kurth
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005393:Fish Diseases / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D031941:Hemorrhagic Septicemia, Viral / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D009500:Neutralization Tests / Q000379:methods*; D029141:Novirhabdovirus / Q000276:immunology*; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D014481:United States; D014730:Veterinary Medicine / Q000379:methods*
chemical_list: D000914:Antibodies, Viral