title: Immunological characteristics and T-cell receptor clonal diversity in children with systemic juvenile idiopathic arthritis undergoing T-cell-depleted autologous stem cell transplantation.
abstract: Children with systemic Juvenile Idiopathic Arthritis (sJIA), the most severe subtype of JIA, are at risk from destructive polyarthritis and growth failure, and corticosteroids as part of conventional treatment can result in osteoporosis and growth delay. In children where there is failure or toxicity from drug therapies, disease has been successfully controlled by T-cell-depleted autologous stem cell transplantation (ASCT). At present, the immunological basis underlying remission after ASCT is unknown. Immune reconstitution of T cells, B cells, natural killer cells, natural killer T cells and monocytes, in parallel with T-cell receptor (TCR) diversity by analysis of the β variable region (TCRVb) complementarity determining region-3 (CDR3) using spectratyping and sequencing, were studied in five children with sJIA before and after ASCT. At time of follow up (mean 11.5 years), four patients remain in complete remission, while one child relapsed within 1 month of transplant. The CD8(+) TCRVb repertoire was highly oligoclonal early in immune reconstitution and re-emergence of pre-transplant TCRVb CDR3 dominant peaks was observed after transplant in certain TCRVb families. Further, re-emergence of pre-ASCT clonal sequences in addition to new sequences was identified after transplant. These results suggest that a chimeric TCR repertoire, comprising T-cell clones developed before and after transplant, can be associated with clinical remission from severe arthritis.
authors.affiliation: Rheumatology Unit, UCL Institute of Child Health, University College London, London, UK.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.forename: Anne M
authors.initials: AM
authors.lastname: Pesenacker
authors.forename: Alka
authors.initials: A
authors.lastname: Stansfield
authors.forename: Douglas
authors.initials: D
authors.lastname: King
authors.forename: Dawn
authors.initials: D
authors.lastname: Barge
authors.forename: Helen E
authors.initials: HE
authors.lastname: Foster
authors.forename: Mario
authors.initials: M
authors.lastname: Abinun
authors.forename: Lucy R
authors.initials: LR
authors.lastname: Wedderburn
mesh_terms: D001171:Arthritis, Juvenile / Q000276:immunology* / Q000628:therapy; D002648:Child; D002999:Clone Cells / Q000276:immunology / Q000378:metabolism; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008212:Lymphocyte Depletion; D008297:Male; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D014182:Transplantation, Autologous
chemical_list: D011948:Receptors, Antigen, T-Cell
keywords: T-cell receptor repertoire clonal diversity; autologous stem cell transplantation; childhood arthritis; immune reconstitution