title: Xanthones from Securidaca inappendiculata exert significant therapeutic efficacy on adjuvant-induced arthritis in mice.
abstract: The study was designed to investigate effects of the xanthones from Securidaca inappendiculata on adjuvant-induced arthritis (AA) mice in vivo. Arthritis severity was evaluated by arthritic score, body weight loss, paw circumference, histological changes and hyperplasia of lymphatic tissues. Plasma samples were collected for estimation of interleukin-1 (IL-1), tumor necrosis factor alpha (TNF-α), monocyte chemotactic protein 1 (MCP-1) and vascular endothelial growth factor (VEGF) using enzyme-linked immunosorbent assay method. The levels of glutathione (GSH), malondialdehyde (MDA), N-acetyl glucosaminidase (NAG) and sialic acid (SA) in liver were assessed by colorimetric method. Xanthones significantly ameliorated the severity of AA indicated by the physical parameters changes, and reverted the abnormal changes of MDA, GSH, NAG and SA in liver. Levels of IL-1, TNF-α, MCP-1 and VEGF reduced dramatically meanwhile. The effects of xanthones on AA were the outcome of the multitargets activities, and probably associated with NF-κB signaling pathway.
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, 138 Xian Lin Road, Nanjing, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zuo
authors.forename: Yan
authors.initials: Y
authors.lastname: Xia
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Chen
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D000818:Animals; D001169:Arthritis, Experimental / Q000188:drug therapy*; D018932:Chemokine CCL2 / Q000097:blood; D005978:Glutathione / Q000378:metabolism; D006965:Hyperplasia / Q000188:drug therapy*; D007375:Interleukin-1 / Q000097:blood; D008099:Liver / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008811:Mice, Inbred DBA; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D028321:Plant Preparations / Q000494:pharmacology*; D031802:Securidaca / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D042461:Vascular Endothelial Growth Factor A / Q000097:blood; D044004:Xanthones / Q000494:pharmacology*
chemical_list: C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D007375:Interleukin-1; D028321:Plant Preparations; D014409:Tumor Necrosis Factor-alpha; D042461:Vascular Endothelial Growth Factor A; D044004:Xanthones; C467480:vascular endothelial growth factor A, mouse; D008315:Malondialdehyde; D000118:Acetylglucosaminidase; D005978:Glutathione; D019158:N-Acetylneuraminic Acid