title: Screening of novel chemical compounds as possible inhibitors of carbonic anhydrase and photosynthetic activity of photosystem II.
abstract: Thirty novel chemical compounds were designed and synthesized expecting that they would be possible inhibitors. From this number eleven were organic bases, twenty-four were their organic derivatives and fourteen were metal complexes. Screening of these chemicals by their action on photosynthetic electron transfer (PET) and carbonic anhydrase (CA) activity (CAA) of photosystem II (PSII), α-CA, as well as β-CA was done. Several groups were revealed among them. Some of them are capable to suppress either one, two, three, or even all of the measured activities. As example, one of the Cu(II)-phenyl sulfonylhydrazone complexes (compound 25) suppresses CAA of α-CA by 88%, CAA of β-CA by 100% inhibition; CAA of PSII by 100% and the PSII photosynthetic activity by 66.2%. The Schiff base compounds (12, 15) and Cu(II)-phenyl sulfonylhydrazone complexes (25, 26) inhibited the CAA and PET of PSII significantly. The obtained data indicate that the PSII donor side is a target of the inhibitory action of these agents. Some physico- or electrochemical properties such as diffusion coefficient, number of transferred electrons, peak potential and heterogeneous standard rate constants of the compounds were determined in nonaqueous media. pKa values were also determined in nonaqueous and aqueous media. Availability in the studied group of novel chemical agents possessing different inhibitory activity allow in future to isolate the "active part" in the structure of the inhibitors responsible for different inhibitory mechanisms, as well as to determine the influence of side substituters on its inhibitory efficiency.
authors.affiliation: Gazi University, Science Faculty, Department of Chemistry, 06500 Ankara, Turkey. Electronic address: mkaracan@gazi.edu.tr.
authors.forename: Mehmet Sayım
authors.initials: MS
authors.lastname: Karacan
authors.affiliation: Institute of Basic Biological Problems, Russian Academy of Sciences, Institutskaya Street 2, Pushchino, Moscow Region 142290, Russia.
authors.forename: Sergei K
authors.initials: SK
authors.lastname: Zharmukhamedov
authors.affiliation: Gazi University, Science Faculty, Department of Chemistry, 06500 Ankara, Turkey.
authors.forename: Serhat
authors.initials: S
authors.lastname: Mamaş
authors.affiliation: Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow 127276, Russia.
authors.forename: Elena V
authors.initials: EV
authors.lastname: Kupriyanova
authors.affiliation: Institute of Basic Biological Problems, Russian Academy of Sciences, Institutskaya Street 2, Pushchino, Moscow Region 142290, Russia.
authors.forename: Alexandr V
authors.initials: AV
authors.lastname: Shitov
authors.affiliation: Institute of Basic Biological Problems, Russian Academy of Sciences, Institutskaya Street 2, Pushchino, Moscow Region 142290, Russia.
authors.forename: Vyacheslav V
authors.initials: VV
authors.lastname: Klimov
authors.affiliation: Ahi Evran University, Dept. of Primary Educ. Fac. Of Educ., 40100 Kırsehir, Turkey.
authors.forename: Neslihan
authors.initials: N
authors.lastname: Özbek
authors.affiliation: Gazi University, Science Faculty, Department of Chemistry, 06500 Ankara, Turkey.
authors.forename: Ümmühan
authors.initials: Ü
authors.lastname: Özmen
authors.affiliation: Gazi University, Science Faculty, Department of Chemistry, 06500 Ankara, Turkey.
authors.forename: Ayla
authors.initials: A
authors.lastname: Gündüzalp
authors.affiliation: Technical University of Berlin, Institute of Chemistry Sekr. PC 14, Max-Volmer-Laboratory of Biophysical Chemistry, Straβe des 17. Juni 135, D-10623 Berlin, Germany.
authors.forename: Franz-Josef
authors.initials: FJ
authors.lastname: Schmitt
authors.affiliation: Gazi University, Science Faculty, Department of Chemistry, 06500 Ankara, Turkey.
authors.forename: Nurcan
authors.initials: N
authors.lastname: Karacan
authors.affiliation: Technical University of Berlin, Institute of Chemistry Sekr. PC 14, Max-Volmer-Laboratory of Biophysical Chemistry, Straβe des 17. Juni 135, D-10623 Berlin, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Friedrich
authors.affiliation: Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow 127276, Russia.
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Los
authors.affiliation: Department de Chimie, Biochimie et Physique, Université du Quebec à Trois Rivières, 3351 Boulevard des Forges, C.P. 500, Québec G9A 5H7, Canada.
authors.forename: Robert
authors.initials: R
authors.lastname: Carpentier
authors.affiliation: Institute of Basic Biological Problems, Russian Academy of Sciences, Institutskaya Street 2, Pushchino, Moscow Region 142290, Russia; Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow 127276, Russia. Electronic address: suleyman.allakhverdiev@gmail.com.
authors.forename: Suleyman I
authors.initials: SI
authors.lastname: Allakhverdiev
mesh_terms: D002257:Carbonic Anhydrase Inhibitors / Q000494:pharmacology*; D002256:Carbonic Anhydrases / Q000378:metabolism*; D004353:Drug Evaluation, Preclinical; D004563:Electrochemistry; D009942:Organometallic Compounds / Q000494:pharmacology; D018532:Pisum sativum / Q000201:enzymology; D055668:Photochemical Processes; D010788:Photosynthesis / Q000187:drug effects*; D045332:Photosystem II Protein Complex / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002257:Carbonic Anhydrase Inhibitors; D009942:Organometallic Compounds; D045332:Photosystem II Protein Complex; D002256:Carbonic Anhydrases
keywords: Carbonic anhydrase; Carbonic anhydrase inhibitors; New inhibitors; Photosynthetic inhibitors; Photosystem II