title: The effect of A 2A receptor antagonist (SCH 442416) on the mRNA expression of Kir 2.1 and Kir 4.1 channels in rat retinal Müller cells under hypoxic conditions in vitro.
abstract: OBJECTIVE
  To investigate the situation of inwardly rectifying potassium channels Kir 2.1 and Kir 4.1 under hypo-xic conditions, and whether the A2A  receptor antagonist could modulate the mRNA expression of Kir 2.1 and Kir 4.1 channels in retinal Müller cells in vitro under hypoxic conditions.
  
  
  METHODS
  Müller cells were treated with 0.1 1, 10 and 100 µM of A2A  receptor antagonist (SCH 442416) under hypoxic conditions for 24 h, and the expression of the Kir 2.1 and Kir 4.1 mRNA channels was examined using real-time polymerase chain reaction (qPCR).
  
  
  RESULTS
  There were no significant changes in the mRNA expression of the Kir 2.1 or Kir 4.1 channels under hypoxic conditions compared with normal conditions after 24 h cultured in vitro. The mRNA expression of Kir 2.1 and Kir 4.1 channels treated with 0.1 µM SCH 442416 under hypoxic conditions were increased, but at higher concentrations of SCH 442416, the mRNA expression of the Kir 2.1 and Kir 4.1 channels decreased.
  
  
  CONCLUSIONS
  The A2A  receptor antagonist (SCH 442416) could increase mRNA expression of the Kir 2.1and Kir 4.1 channels in Müller cells to protect the retinal neurons in vitro under hypoxic conditions.
authors.affiliation: Department of Ophthalmology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Kang
authors.forename: Jun
authors.initials: J
authors.lastname: Yu
authors.forename: Yan
authors.initials: Y
authors.lastname: Wei
authors.forename: Peiquan
authors.initials: P
authors.lastname: Zhao
mesh_terms: D058917:Adenosine A2 Receptor Antagonists / Q000494:pharmacology*; D000818:Animals; D015687:Cell Hypoxia; D002478:Cells, Cultured; D063928:Ependymoglial Cells / Q000187:drug effects* / Q000378:metabolism; D007150:Immunohistochemistry; D024661:Potassium Channels, Inwardly Rectifying / Q000235:genetics*; D011720:Pyrazoles / Q000494:pharmacology*; D011743:Pyrimidines / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000098854:Kcnj10 Channel
chemical_list: D058917:Adenosine A2 Receptor Antagonists; C575188:KCNJ2 protein, rat; D000098854:Kcnj10 Channel; D024661:Potassium Channels, Inwardly Rectifying; D011720:Pyrazoles; D011743:Pyrimidines; D012333:RNA, Messenger; C416650:SCH 442416