title: Coat protein-mediated transgenic resistance of peanut (Arachis hypogaea L.) to peanut stem necrosis disease through Agrobacterium-mediated genetic transformation.
abstract: The absence of resistance genes against biotic stresses like Tobacco streak virus (TSV) within compatible peanut germplasm necessitates the deployment of genetic engineering strategy to develop transgenic resistance. Transgenic resistance in peanut (Arachis hypogaea L.) to peanut stem necrosis disease caused by TSV was obtained by transferring coat protein (CP) gene of TSV through Agrobacterium-mediated transformation of de-embryonated cotyledons and immature leaves of peanut cultivars Kadiri 6 (K6) and Kadiri 134 (K134). Integration of the transgene in T1, T2 and T3 generations were confirmed by PCR with gene-specific primers. On the basis of segregation analysis of the PCR amplicons, homozygosity was confirmed in progeny from five transgenic lines. Six transgenic plants from three different single copy transgenic lines homozygous for the transgene were selected for challenge inoculation in T3 generations. The transgenic lines remained symptomless throughout and showed traces or no systemic accumulation of virus indicating the tolerance/resistance to the TSV infection. CP gene expression was observed in transgenic lines by RT-PCR, real-time PCR and ELISA. The findings provide an effective strategy for developing peanut with resistance to peanut stem necrosis disease.
authors.affiliation: Directorate of Groundnut Research, Ivnagar Road, Post Box 5, Junagadh, 362 001 Gujarat India.
authors.forename: Reetu
authors.initials: R
authors.lastname: Mehta
authors.affiliation: Directorate of Groundnut Research, Ivnagar Road, Post Box 5, Junagadh, 362 001 Gujarat India.
authors.forename: Thankappan
authors.initials: T
authors.lastname: Radhakrishnan
authors.affiliation: Directorate of Groundnut Research, Ivnagar Road, Post Box 5, Junagadh, 362 001 Gujarat India.
authors.forename: Abhay
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Directorate of Groundnut Research, Ivnagar Road, Post Box 5, Junagadh, 362 001 Gujarat India.
authors.forename: Reena
authors.initials: R
authors.lastname: Yadav
authors.affiliation: Directorate of Groundnut Research, Ivnagar Road, Post Box 5, Junagadh, 362 001 Gujarat India.
authors.forename: Jentilal R
authors.initials: JR
authors.lastname: Dobaria
authors.affiliation: Directorate of Groundnut Research, Ivnagar Road, Post Box 5, Junagadh, 362 001 Gujarat India.
authors.forename: Palanisamy P
authors.initials: PP
authors.lastname: Thirumalaisamy
authors.affiliation: Division of Plant Pathology, Advanced Centre for Plant Virology, Indian Agricultural Research Institute, New Delhi, 110 012 India.
authors.forename: Rakesh K
authors.initials: RK
authors.lastname: Jain
authors.affiliation: Division of Plant Pathology, Advanced Centre for Plant Virology, Indian Agricultural Research Institute, New Delhi, 110 012 India.
authors.forename: Phaneedra
authors.initials: P
authors.lastname: Chigurupati
keywords: Agrobacterium-mediated genetic transformation; Arachis hypogaea; Coat protein-mediated transgenic resistance; Peanut stem necrosis disease