title: Inhibitory effects of ethyl acetate-soluble fraction from morus alba on lipid accumulation in 3T3-L1 cells.
abstract: Fruits of mulberry (Morus alba) have been widely used for therapeutic purposes in Asian countries for centuries. Treatment of 3T3-L1 cells with ethanolic extracts of M. alba decreased adipocyte differentiation at 100 microg/mL by 18.6%. Treatment suppressed mRNA levels of PPARgamma and C/EBPalpha expression in 3T3-L1 cells. However, the extract did not change free glycerol release from mature adipocytes. Thus, M. alba inhibited lipid accumulation by regulating transcription factors in 3T3-L1 adipocytes without a lipolytic effect. Among the soluble- fractions, the ethyl acetate-soluble fraction had the highest antiadipogenic effects on 3T3-L1 cells. This fraction decreasing intracellular lipid accumulation by 38.5% in response to treatment with 100 microg/mL. In addition, HPLC analysis of the ethyl acetate-soluble fraction of M. alba contained 167.7 microM of protocatechulic acid in 1 mg/mL of fraction, which inhibited lipid accumulation by 44.8% in response to treatment with 100 microM. From these results, M. alba is a possible candidate for regulating lipid accumulation in obesity.
authors.affiliation: Plant Resources Research Institute, Duksung Women's University, Seoul, Republic of Korea.
authors.forename: Hee-Sook
authors.initials: HS
authors.lastname: Park
authors.affiliation: Department of Food Science and Technology, Sejong University, Seoul, Republic of Korea.
authors.forename: Soon-Mi
authors.initials: SM
authors.lastname: Shim
authors.affiliation: Plant Resources Research Institute, Duksung Women's University, Seoul, Republic of Korea.
authors.forename: Gun-Hee
authors.initials: GH
authors.lastname: Kim
mesh_terms: D041721:3T3-L1 Cells; D000085:Acetates; D000818:Animals; D050356:Lipid Metabolism / Q000187:drug effects*; D051379:Mice; D031625:Morus / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000085:Acetates; D010936:Plant Extracts; C007650:ethyl acetate