title: H(N), N(H), C (α), C (β), and methyl group assignments of filamin multidomain fragments IgFLNc4-5 and IgFLNa3-5.
abstract: Filamins regulate the actin cytoskeleton by cross-linking actin filaments, linking the cytoskeleton to the cell membrane, and through interaction with numerous binding partners such as intracellular signalling molecules, ion channels, receptors, enzymes and transcription factors. The rod region of filamins consists of 24 immunoglubulin (Ig)-like repeats, for some of which the functional unit is a domain pair. Our aim is to study filamin Ig domain-domain interactions and quaternary arrangement as well as to locate peptide binding sites on domain assemblies. We report here the H(N), N(H), C(α), C(β), and methyl group assignments of filamin A three-domain fragment IgFLNa3-5 and filamin C two-domain fragment IgFLNc4-5.
authors.affiliation: Program in Structural Biology and Biophysics, Institute of Biotechnology, University of Helsinki, Viikinkaari 1, P.O. Box 65, 00014, Helsinki, Finland.
authors.forename: Helena
authors.initials: H
authors.lastname: Tossavainen
authors.forename: Jonne
authors.initials: J
authors.lastname: Seppälä
authors.forename: Ritika
authors.initials: R
authors.lastname: Sethi
authors.forename: Tero
authors.initials: T
authors.lastname: Pihlajamaa
authors.forename: Perttu
authors.initials: P
authors.lastname: Permi
mesh_terms: D064448:Filamins / Q000737:chemistry*; D006801:Humans; D007136:Immunoglobulins / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D010446:Peptide Fragments / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D020449:Repetitive Sequences, Amino Acid
chemical_list: D064448:Filamins; D007136:Immunoglobulins; D010446:Peptide Fragments