title: [Sample pretreatment methods of pesticide residues in Panax notoginseng of Chinese traditional medicine].
abstract: Two sample pretreatment methods of pesticide residues in Panax notoginseng of Chinese traditional medicine were developed. For Method I, the residues were extracted from homogenized tissue with n-hexane-dichloromethane (6:4) by means of ultrasonication, the crude extract was purified by an Envi-carb/NH2 solid-phase extraction (SPE) column. For Method II, matrix solid-phase dispersion (MSPD) technique was used for extracting and cleaning up. The eluates were concentrated by rotary evaporation, and then were redissolved in dichloromethane prior to GC-MS determination. The determination was performed in selected ion monitoring (SIM) mode with the external calibration for quantitative analysis. Under the optimal conditions, the results indicated that the methods are easier and faster, the recoveries of method I for the spiked standards at concentration of 0.01, 0.5, and 2.0 mg x kg(-1) were 81.90%-102.10% with the relative standard deviations (RSDs) of 3.60%-7.10%. The recoveries of method II were 96.26%-104.20% with the RSDs of 3.52%-7.94%. The detection limits (S/N) for residues of pesticides were in the range of 0.48-1.34 ng x g(-1). The results indicated that these multiresidue analysis methods can meet the requirements for determination of residue pesticides and can be appropriate for trace analysis of residue pesticides in Panax notoginseng.
authors.affiliation: Department of Chemistry, Honghe College, Mengzi 661100, China.
authors.forename: Na
authors.initials: N
authors.lastname: Wu
authors.affiliation: Department of Chemistry, Honghe College, Mengzi 661100, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Chemistry, Honghe College, Mengzi 661100, China.
authors.forename: He-Ping
authors.initials: HP
authors.lastname: Yan
authors.affiliation: Department of Chemistry, Honghe College, Mengzi 661100, China.
authors.forename: Ai-Ping
authors.initials: AP
authors.lastname: Fan
authors.affiliation: Department of Chemistry, Honghe College, Mengzi 661100, China.
authors.forename: Du-Shu
authors.initials: DS
authors.lastname: Huang
authors.affiliation: Department of Chemistry, Honghe College, Mengzi 661100, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Department of Chemistry, Honghe College, Mengzi 661100, China.
authors.forename: Xian-Lan
authors.initials: XL
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Honghe College, Mengzi 661100, China.
authors.forename: Shi-Juan
authors.initials: SJ
authors.lastname: Xu
authors.affiliation: Department of Chemistry, Honghe College, Mengzi 661100, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Shi
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D006586:Hexanes / Q000737:chemistry; D008752:Methylene Chloride / Q000737:chemistry; D053723:Panax notoginseng / Q000737:chemistry*; D010573:Pesticide Residues / Q000032:analysis*; D052616:Solid Phase Extraction; D012997:Solvents
chemical_list: D006586:Hexanes; D010573:Pesticide Residues; D012997:Solvents; C026385:n-hexane; D008752:Methylene Chloride