title: [A hypothetical gene pair, ssl2138 and sll11092, constitutes a functional TA system on the chromosome of Synechocystis PCC 6803].
abstract: OBJECTIVE
  We characterized the hypothetical gene pair ssl2138 and sll1092 that constitute a vapBC-family toxin-antitoxin (TA) system on the chromosome of Synechocystis PCC 6803.
  
  
  METHODS
  An RT-PCR analysis was conducted to confirm the co-transcription of this gene pair, a selection-expression system was constructed to demonstrate the effect of their encoded proteins on E. coli growth. To validate interaction between these two proteins, an affinity capture analysis was performed.
  
  
  RESULTS
  ssl2138 and sll1092 were co-transcribed under normal growth conditions. Ectopic expression of sll1092 inhibited the growth of E. coli, which could be overcome by simultaneous or subsequent expression of ssl2138. Both proteins Ssl2138 and Sll1092 in vivo formed a protein complex due to their specific interaction.
  
  
  CONCLUSIONS
  ssl2138 and sll1092 constitute a functional vapBC-family TA system of Synechocystis PCC 6803.
authors.affiliation: School of the Environment, Jiangsu University, 212013 Zhenjiang, China. 306502@ujs.edu.cn
authors.forename: Degang
authors.initials: D
authors.lastname: Ning
authors.affiliation: School of the Environment, Jiangsu University, 212013 Zhenjiang, China.
authors.forename: Weifei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: School of the Environment, Jiangsu University, 212013 Zhenjiang, China.
authors.forename: Yaru
authors.initials: Y
authors.lastname: Qian
mesh_terms: D000992:Antitoxins / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism*; D002876:Chromosomes, Bacterial / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D011485:Protein Binding; D046939:Synechocystis / Q000235:genetics / Q000378:metabolism*
chemical_list: D000992:Antitoxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins