title: Cytological, microbiological and therapeutic aspects of systemic infection in a dog caused by the fungus Phialosimplex caninus.
abstract: A seven-year-old immunocompetent dog presenting with lymphadenopathy, mesenteric masses and splenic nodules was diagnosed with Phialosimplex caninus infection. Cytology of a mesenteric mass aspirate demonstrated few intact cells but numerous variably sized fungal cells and rare hyphal fragments. The identity of the cultured fungus was confirmed by DNA sequencing. Itraconazole therapy improved clinical signs, but the fungus was reisolated at follow-up. P. caninus systemic infection should be suspected in dogs presenting with lymphadenopathy and splenomegaly.
authors.affiliation: University of Alberta Microfungus Collection and Herbarium, Edmonton, AB, Canada.
authors.forename: Lynne
authors.initials: L
authors.lastname: Sigler
authors.affiliation: Mississauga Oakville Veterinary Emergency Hospital, Oakville, ON, Canada.
authors.forename: Beth
authors.initials: B
authors.lastname: Hanselman
authors.affiliation: Animal Health Laboratory, University of Guelph, ON, Canada.
authors.forename: Kristiina
authors.initials: K
authors.lastname: Ruotsalo
authors.affiliation: Public Health Ontario Laboratories, Public Health Ontario, Etobicoke, ON, Canada.
authors.forename: George
authors.initials: G
authors.lastname: Kar Tsui
authors.affiliation: Public Health Ontario Laboratories, Public Health Ontario, Etobicoke, ON, Canada.
authors.forename: Susan
authors.initials: S
authors.lastname: Richardson
keywords: Canine; ITS sequencing; Lymphadenopathy; Mycosis; Phialosimplex caninus; Splenomegaly