title: Wound-induced expression of DEFECTIVE IN ANTHER DEHISCENCE1 and DAD1-like lipase genes is mediated by both CORONATINE INSENSITIVE1-dependent and independent pathways in Arabidopsis thaliana.
abstract: Endogenous JA production is not necessary for wound-induced expression of JA-biosynthetic lipase genes such as DAD1 in Arabidopsis. However, the JA-Ile receptor COI1 is often required for their JA-independent induction. Wounding is a serious event in plants that may result from insect feeding and increase the risk of pathogen infection. Wounded plants produce high amounts of jasmonic acid (JA), which triggers the expression of insect and pathogen resistance genes. We focused on the transcriptional regulation of DEFECTIVE IN ANTHER DEHISCENCE1 and six of its homologs including DONGLE (DGL) in Arabidopsis, which encode lipases involved in JA biosynthesis. Plants constitutively expressing DAD1 accumulated a higher amount of JA than control plants after wounding, indicating that the expression of these lipase genes contributes to determining JA levels. We found that the expression of DAD1, DGL, and other DAD1-LIKE LIPASE (DALL) genes is induced upon wounding. Some DALLs were also expressed in unwounded leaves. Further experiments using JA-biosynthetic and JA-response mutants revealed that the wound induction of these genes is regulated by several distinct pathways. DAD1 and most of its homologs other than DALL4 were fully induced without relying on endogenous JA-Ile production and were only partly affected by JA deficiency, indicating that positive feedback by JA is not necessary for induction of these genes. However, DAD1 and DGL required CORONATINE INSENSITIVE1 (COI1) for their expression, suggesting that a molecule other than JA might act as a regulator of COI1. Wound induction of DALL1, DALL2, and DALL3 did not require COI1. This differential regulation of DAD1 and its homologs might explain their functions at different time points after wounding.
authors.affiliation: Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601, Japan.
authors.forename: Izabela
authors.initials: I
authors.lastname: Ruduś
authors.forename: Haruka
authors.initials: H
authors.lastname: Terai
authors.forename: Takafumi
authors.initials: T
authors.lastname: Shimizu
authors.forename: Hisae
authors.initials: H
authors.lastname: Kojima
authors.forename: Kazuki
authors.initials: K
authors.lastname: Hattori
authors.forename: Yuka
authors.initials: Y
authors.lastname: Nishimori
authors.forename: Hironaka
authors.initials: H
authors.lastname: Tsukagoshi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kamiya
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Seo
authors.forename: Kenzo
authors.initials: K
authors.lastname: Nakamura
authors.forename: Jan
authors.initials: J
authors.lastname: Kępczyński
authors.forename: Sumie
authors.initials: S
authors.lastname: Ishiguro
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D008049:Lipase / Q000235:genetics / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism / Q000494:pharmacology*; D010741:Phospholipases A / Q000235:genetics* / Q000378:metabolism; D054466:Phospholipases A1 / Q000235:genetics* / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology*; D018515:Plant Leaves / Q000235:genetics / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D014947:Wounds and Injuries
chemical_list: D029681:Arabidopsis Proteins; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; C112279:COI1 protein, Arabidopsis; D003517:Cyclopentanes; C473611:MYC2 protein, Arabidopsis; D054883:Oxylipins; D010937:Plant Growth Regulators; D012333:RNA, Messenger; D018749:RNA, Plant; C011006:jasmonic acid; C551458:DONGLE protein, Arabidopsis; D008049:Lipase; C445094:DAD1 protein, Arabidopsis; D010741:Phospholipases A; D054466:Phospholipases A1