title: Kinetics study of pyridine biodegradation by a novel bacterial strain, Rhizobium sp. NJUST18.
abstract: Biodegradation of pyridine by a novel bacterial strain, Rhizobium sp. NJUST18, was studied in batch experiments over a wide concentration range (from 100 to 1,000 mg l(-1)). Pyridine inhibited both growth of Rhizobium sp. NJUST18 and biodegradation of pyridine. The Haldane model could be fitted to the growth kinetics data well with the kinetic constants μ* = 0.1473 h(-1), K s = 793.97 mg l(-1), K i = 268.60 mg l(-1) and S m = 461.80 mg l(-1). The true μ max, calculated from μ*, was found to be 0.0332 h(-1). Yield coefficient Y X/S depended on S i and reached a maximum of 0.51 g g(-1) at S i of 600 mg l(-1). V max was calculated by fitting the pyridine consumption data with the Gompertz model. V max increased with initial pyridine concentration up to 14.809 mg l(-1) h(-1). The q S values, calculated from [Formula: see text], were fitted with the Haldane equation, yielding q Smax = 0.1212 g g(-1) h(-1) and q* = 0.3874 g g(-1) h(-1) at S m' = 507.83 mg l(-1), K s' = 558.03 mg l(-1), and K i' = 462.15 mg l(-1). Inhibition constants for growth and degradation rate value were in the same range. Compared with other pyridine degraders, μ max and S m obtained for Rhizobium sp. NJUST18 were relatively high. High K i and K i' values and extremely high K s and K s' values indicated that NJUST18 was able to grow on pyridine within a wide concentration range, especially at relatively high concentrations.
authors.affiliation: Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094, Jiangsu Province, China, shenjinyou@mail.njust.edu.cn.
authors.forename: Jinyou
authors.initials: J
authors.lastname: Shen
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Dan
authors.initials: D
authors.lastname: Chen
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Liu
authors.forename: Libin
authors.initials: L
authors.lastname: Zhang
authors.forename: Xiuyun
authors.initials: X
authors.lastname: Sun
authors.forename: Jiansheng
authors.initials: J
authors.lastname: Li
authors.forename: Huiping
authors.initials: H
authors.lastname: Bi
authors.forename: Lianjun
authors.initials: L
authors.lastname: Wang
mesh_terms: D001673:Biodegradation, Environmental; D007700:Kinetics; D008954:Models, Biological; D011725:Pyridines / Q000378:metabolism*; D012231:Rhizobium / Q000254:growth & development*
chemical_list: D011725:Pyridines; C023666:pyridine