title: Application of a glycoproteomics-based biomarker development method: alteration in glycan structure on colony stimulating factor 1 receptor as a possible glycobiomarker candidate for evaluation of liver cirrhosis.
abstract: The importance of diagnosis and therapies for liver cirrhosis (LC) is indisputable. Thus, a reliable method for monitoring the progression of liver fibrosis and resultant LC is urgently needed. Previously, using a lectin-assisted glycoproteomic method, we identified 26 serum glycoproteins as promising glycobiomarker candidates for monitoring the progression of liver diseases. In this study, we identified colony stimulating factor 1 receptor (CSF1R) as a promising LC marker candidate and then established Wisteria floribunda agglutinin (WFA)-reactive CSF1R (WFA(+)-CSF1R) as a novel possible glycobiomarker candidate by utilizing a glycoproteomics-based strategy. The serum level of WFA(+)-CSF1R in patients with hepatitis C virus (HCV)-infected liver disease was measured by an antibody-lectin sandwich ELISA. In a proof-of-concept experiment of the strategy preceding to future clinical studies, LC patients showed a high serum WFA(+)-CSF1R level in selected samples (P = 1.3 × 10(-17)). This result suggests WFA(+)-CSF1R is a possible biomarker candidate for evaluation of LC. Our results verified feasibility of this strategy for glycobiomarker development.
authors.affiliation: Research Center for Medical Glycoscience (RCMG), National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Ocho
authors.forename: Akira
authors.initials: A
authors.lastname: Togayachi
authors.forename: Etsuko
authors.initials: E
authors.lastname: Iio
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kaji
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kuno
authors.forename: Maki
authors.initials: M
authors.lastname: Sogabe
authors.forename: Masaaki
authors.initials: M
authors.lastname: Korenaga
authors.forename: Masanori
authors.initials: M
authors.lastname: Gotoh
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Ikehara
authors.forename: Masashi
authors.initials: M
authors.lastname: Mizokami
authors.forename: Hisashi
authors.initials: H
authors.lastname: Narimatsu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D002236:Carbohydrate Conformation; D004797:Enzyme-Linked Immunosorbent Assay; D005240:Feasibility Studies; D005260:Female; D006023:Glycoproteins / Q000097:blood* / Q000737:chemistry; D019694:Hepatitis B, Chronic / Q000097:blood; D019698:Hepatitis C, Chronic / Q000097:blood; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood* / Q000175:diagnosis; D008297:Male; D008875:Middle Aged; D037121:Plant Lectins / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry; D040081:Protein Array Analysis; D040901:Proteomics; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000097:blood* / Q000737:chemistry; D034781:Receptors, N-Acetylglucosamine / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D006023:Glycoproteins; D037121:Plant Lectins; D011134:Polysaccharides; D034781:Receptors, N-Acetylglucosamine; C040129:wisteria lectin; D016186:Receptor, Macrophage Colony-Stimulating Factor