title: Prevalence of Salmonella serovars, Listeria monocytogenes, and Escherichia coli O157:H7 in Mediterranean ready-to-eat meat products in Jordan.
abstract: The presence of Salmonella, Listeria monocytogenes, and Escherichia coli O157:H7 in ready-to-eat (RTE) meat products is considered a major concern for food control authorities worldwide. The aims of this study were to determine (i) the prevalence of Salmonella, L. monocytogenes, and E. coli O157:H7 in Mediterranean RTE chicken and beef (CB) products sold in Jordanian restaurants and (ii) the susceptibility of the isolates to antibiotics. A total of 1,028 samples of various types of RTE CB products (550 RTE chicken and 478 RTE beef products) were analyzed by methods described by the International Organization for Standardization followed by molecular confirmation of the isolates. The VITEK2 automated system was used for testing antibiotic susceptibility of the isolates. The overall prevalence of Salmonella serovars in RTE CB products was 0.5%, with 0.8 and 0.2% in RTE chicken and RTE beef, respectively. The overall prevalence of L. monocytogenes in RTE CB products was 2%, with 2.7 and 1.5% in RTE chicken and RTE beef products, respectively. E. coli O157:H7 was not isolated from any of the tested samples. Multidrug-resistant Salmonella and L. monocytogenes isolates were found. The majority of Salmonella isolates were sensitive to most of the tested antibiotics, and all of the isolates were resistant to more than one antibiotic. Similarly, more than 85% of L. monocytogenes isolates were sensitive to nine antibiotics, and the majority of L. monocytogenes isolates were resistant to fosfomycin and oxacillin.
authors.affiliation: Department of Nutrition and Food Technology, Jordan University of Science and Technology, Irbid 22110, Jordan.
authors.forename: Tareq M
authors.initials: TM
authors.lastname: Osaili
authors.affiliation: Department of Nutrition and Food Technology, Jordan University of Science and Technology, Irbid 22110, Jordan.
authors.forename: Anas A
authors.initials: AA
authors.lastname: Al-Nabulsi
authors.affiliation: Department of Nutrition and Food Technology, Jordan University of Science and Technology, Irbid 22110, Jordan.
authors.forename: Reyad R
authors.initials: RR
authors.lastname: Shaker
authors.affiliation: Department of Nutrition and Food Technology, Jordan University of Science and Technology, Irbid 22110, Jordan; Department of Biotechnology and Genetic Engineering, Jordan University of Science and Technology, Irbid 22110, Jordan.
authors.forename: Ziad W
authors.initials: ZW
authors.lastname: Jaradat
authors.affiliation: Department of Nutrition and Food Technology, Jordan University of Science and Technology, Irbid 22110, Jordan.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Taha
authors.affiliation: Jordan Food and Drug Administration, Amman, Jordan.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Al-Kherasha
authors.affiliation: Municipality of Greater Amman, Amman, Jordan.
authors.forename: Mervet
authors.initials: M
authors.lastname: Meherat
authors.affiliation: Department of Food Science, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2.
authors.forename: Richard
authors.initials: R
authors.lastname: Holley
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002417:Cattle; D002645:Chickens; D015169:Colony Count, Microbial; D024881:Drug Resistance, Bacterial; D019453:Escherichia coli O157 / Q000187:drug effects / Q000302:isolation & purification*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D059022:Food Safety; D007597:Jordan / Q000453:epidemiology; D008089:Listeria monocytogenes / Q000187:drug effects / Q000302:isolation & purification*; D008461:Meat Products / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D015995:Prevalence; D012475:Salmonella / Q000187:drug effects / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents