title: [Effect of Hanfangji compound on proliferation of hepatic stellate cells and expressions of TTR, ITIH1 and SERPINF2].
abstract: OBJECTIVE
  To observe and compare the effects of Hanfangji Compound and IFN-gamma on expressions of transthyretin (TTR) , inter-alpha inhibitor H1 (ITIH1) and serpin peptidase inhibitor clade F member 2 (SERPINF2) of hepatic stellate cells (HSC-T6).
  
  
  METHODS
  Hanfangji Compound and IFN-gammaof different concentrations were used in hepatic stellate cell-T6 (HSC-T6) for 48 h. Flow cytometer was used to detect the effects of Hanfangji Compound and IFN-gamma on HSC proliferation. RT-PCR method was adopted to detect mRNA expressions of TFR, ITIH1 and SERPINF2. TTR, ITIH1 and SERPINF2 secretions were detected by ELISA. The protein localizations of TTR, ITIH1 and SERPINF2 were examined by immune fluorescence. The protein expression of TfR and ITIHI were determined by Western blot.
  
  
  RESULTS
  After Hanfangji Compound and IFN-gamma were adopted in HSC-T6, compared with the control group, the cell proliferation was inhibited obviously (P < 0. 05) , protein expressions of TTR, ITIH1 and SERPINF2 and mRNA expression increased significantly, with certain correlation with concentrations of Hanfangji Compound. The 2. 5 g L-I Hanfangji Compound group was superior to the IFN-gamma group (P <0. 05).
  
  
  CONCLUSIONS
  Hanfangji Compound can inhibit HSC proliferation, upregulated TTR, ITIH1 and SERPINF2 proteins and mRNA expression, which may be one of mechanisms of anti-hepatic fibrosis of Hanfangji Compound.
authors.affiliation: Department of Gastroenterology, Jinan Military General Hospital of PLA, Jinan 250031, China.
authors.forename: Yu-Jia
authors.initials: YJ
authors.lastname: Feng
authors.affiliation: Department of Gastroenterology, Jinan Military General Hospital of PLA, Jinan 250031, China.
authors.forename: Yao-Jun
authors.initials: YJ
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Jinan Military General Hospital of PLA, Jinan 250031, China.
authors.forename: Fu-Cheng
authors.initials: FC
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, Jinan Military General Hospital of PLA, Jinan 250031, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Department of Gastroenterology, Jinan Military General Hospital of PLA, Jinan 250031, China.
authors.forename: Qi-Zhen
authors.initials: QZ
authors.lastname: Quan
mesh_terms: D000510:Alpha-Globulins / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D055166:Hepatic Stellate Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D017457:Receptors, Albumin / Q000235:genetics / Q000378:metabolism*; D000979:alpha-2-Antiplasmin / Q000235:genetics / Q000378:metabolism*
chemical_list: D000510:Alpha-Globulins; D004365:Drugs, Chinese Herbal; D017457:Receptors, Albumin; C534949:SERPINF2 protein, human; D000979:alpha-2-Antiplasmin; C091986:transthyretin receptor; C011877:inter-alpha-inhibitor