title: [Inhibition of Fasudil on lipopolysaccharide-induced TNF-α and IL-1β expressions through TLR4 pathway in murine BV-2 cells in vitro].
abstract: OBJECTIVE
  To evaluate the effects of Fasudil on the production of proinflammatory cytokines in lipopolysaccharide (LPS)-stimulated BV-2 microglia cells.
  
  
  METHODS
  The BV-2 cells cultured in vitro were divided into PBS control group, LPS stimulation group and LPS plus Fasudil group. The production of TNF-α and IL-1β was measured by ELISA. The release of NO was checked by Griess reagent assay. Toll-like receptor 2 (TLR2) and TLR4 expressions were determined by flow cytometry.
  
  
  RESULTS
  The stimulation of LPS significantly increased the production of TNF-α, IL-1β and NO as well as TLR4 protein expression in BV-2 cells. Fasudil attenuated NO production, and reduced the release of TNF-α and IL-1β in LPS-stimulated BV-2 cells. Interestingly, Fasudil also decreased TLR4 expression.
  
  
  CONCLUSIONS
  Fasudil can suppress the production of TNF-α, IL-1β and NO of microglia cells induced by LPS, which may be associated with the down-regulation of TLR4 pathway.
authors.affiliation: Institute of Brain Science, Shanxi Datong University, Datong 037009, China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Encephalopathy, Third Hospital, Shanxi University of Traditional Chinese Medicine, Taiyuan 030024, China.
authors.forename: Xingwang
authors.initials: X
authors.lastname: Yang
authors.affiliation: Institute of Brain Science, Shanxi Datong University, Datong 037009, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Institute of Brain Science, Shanxi Datong University, Datong 037009, China.
authors.forename: Jiezhong
authors.initials: J
authors.lastname: Yu
authors.affiliation: Institute of Brain Science, Shanxi Datong University, Datong 037009, China.
authors.forename: Chunyun
authors.initials: C
authors.lastname: Liu
authors.affiliation: Institute of Brain Science, Shanxi Datong University, Datong 037009, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Feng
authors.affiliation: Department of Encephalopathy, Third Hospital, Shanxi University of Traditional Chinese Medicine, Taiyuan 030024, China.
authors.forename: Junlian
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Neurology, Huashan Hospital, Fudan University, Shanghai 200025, China.
authors.forename: Baoguo
authors.initials: B
authors.lastname: Xiao
authors.affiliation: Institute of Brain Science, Shanxi Datong University, Datong 037009; Department of Encephalopathy, Third Hospital, Shanxi University of Traditional Chinese Medicine, Taiyuan 030024, China.
authors.forename: Cungen
authors.initials: C
authors.lastname: Ma
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects; D053583:Interleukin-1beta / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D017628:Microglia / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D051195:Toll-Like Receptor 2 / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D018696:Neuroprotective Agents; C493486:Tlr2 protein, mouse; C493487:Tlr4 protein, mouse; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; C049347:fasudil