title: An attractive way of egg white protein by-product use for producing of novel anti-hypertensive peptides.
abstract: The aim of this study was to (i) examine how enzymatic hydrolysis with a non-commercially available proteinase of fig-leaf gourd fruit (Cucurbita ficifolia) increased the use value of egg white protein preparations, generated as byproducts in the industrial process of lysozyme and cystatin isolation from egg white, and (ii) evaluate the inhibition of angiotensin I-converting enzyme (ACE) by the obtained hydrolysates. Purification procedures including membrane filtration, gel filtration chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC) led to the production of several peptide fractions. Two novel ovalbumin-derived tetrapeptides: SWVE (f 148-151) and DILN (f 86-89) with ACE inhibitory activity were obtained. Study of their inhibitory kinetics revealed a non-competitive binding mode, with an IC50 value against ACE of 33.88 and 73.44 μg for SWVE and DILN, respectively. Synthetic peptides which were designed on the basis of peptide SWVE were examined. A tripeptide sequence of SWV revealed the strongest ACE-inhibitory activity.
authors.affiliation: Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Chełmońskiego 37/41, 51-630 Wroclaw, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Pokora
authors.affiliation: Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Chełmońskiego 37/41, 51-630 Wroclaw, Poland. Electronic address: aleksandra.zambrowicz@up.wroc.pl.
authors.forename: A
authors.initials: A
authors.lastname: Zambrowicz
authors.affiliation: Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Chełmońskiego 37/41, 51-630 Wroclaw, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Dąbrowska
authors.affiliation: Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Chełmońskiego 37/41, 51-630 Wroclaw, Poland.
authors.forename: E
authors.initials: E
authors.lastname: Eckert
authors.affiliation: Faculty of Chemistry, University of Wroclaw, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Setner
authors.affiliation: Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Chełmońskiego 37/41, 51-630 Wroclaw, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Szołtysik
authors.affiliation: Faculty of Chemistry, University of Wroclaw, Poland.
authors.forename: Z
authors.initials: Z
authors.lastname: Szewczuk
authors.affiliation: Department of Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wrocław, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Zabłocka
authors.affiliation: Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Chełmońskiego 37/41, 51-630 Wroclaw, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Polanowski
authors.affiliation: Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Chełmońskiego 37/41, 51-630 Wroclaw, Poland.
authors.forename: T
authors.initials: T
authors.lastname: Trziszka
authors.affiliation: Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Chełmońskiego 37/41, 51-630 Wroclaw, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Chrzanowska
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry; D000959:Antihypertensive Agents / Q000627:therapeutic use*; D004527:Egg Proteins / Q000737:chemistry*; D006868:Hydrolysis; D010455:Peptides / Q000737:chemistry*
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D004527:Egg Proteins; D010455:Peptides
keywords: Angiotensin I-converting enzyme inhibitory activity; Bioactive peptides; Hydrolysis; Protein by-product; Proteinase of Cucurbita ficifolia