title: Nanosilver against fungi. Silver nanoparticles as an effective biocidal factor.
abstract: The work presents a method of obtaining an aqueous raspberry extract as well as its physicochemical and analytical characteristics. The paper also contains a description of the method of preparation of nanosilver suspensions based on this extract. The raspberry extract served as a source of phenolic compounds which acted as both reducing and stabilizing agents. Suspensions of silver nanoparticles were obtained with the use of chemical reduction method. The silver ions concentration, pH value and temperature of samples incubation were independent variables. The next step of the research was to measure the antifungal activity of the received silver nanoparticles as well as to perform a mycological efficacy resistance analysis of the tested preparations in relation to different concentrations of nanostructured silver. Tests were conducted in compliance with the Eucast guidelines. The results of microbiological study of (the samples') biocidal effect against Cladosporium cladosporoides and Aspergillus niger are described. It was found that using nanosilver suspension at the concentration of 50 ppm inhibited the growth of Cladosporium cladosporoides and Aspergillus niger by 90% and 70%, respectively.
authors.affiliation: Cracow University of Technology, Institute of Inorganic Chemistry and Technology, Kraków, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Pulit
authors.affiliation: Cracow University of Technology, Institute of Inorganic Chemistry and Technology, Kraków, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Banach
authors.affiliation: Silesian Environmental Ph.D. Studies Centre, Central Mining Institute, Katowice, Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Szczygłowska
authors.affiliation: The Implementing Company Kraków, Poland.
authors.forename: Mirosław
authors.initials: M
authors.lastname: Bryk
mesh_terms: D001234:Aspergillus niger / Q000187:drug effects; D002963:Cladosporium / Q000187:drug effects; D005638:Fruit / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D010636:Phenols / Q000008:administration & dosage / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry*; D012834:Silver / Q000008:administration & dosage / Q000737:chemistry*; D014867:Water
chemical_list: D010636:Phenols; D010936:Plant Extracts; D014867:Water; D012834:Silver