title: Molecular characterization and expression analysis of a peroxiredoxin 1 cDNA from Korean rose bitterling (Rhodeus uyekii).
abstract: Peroxiredoxins (Prxs), also known as natural killer cell enhancing factors in fish, role as antioxidant proteins and participate in a variety of biological processes, including H2O2-mediated cell signaling, molecular chaperoning, and mitochondrial function. In this study, we isolated and characterized a Prx 1 cDNA from the Korean rose bitterling Rhodeus uyekii, and designated it RuPrx 1. The RuPrx 1 cDNA encodes a 197-amino-acid polypeptide that belongs to the class of typical 2-Cys Prxs that contain peroxidatic and resolving cysteines. The deduced RuPrx 1 protein shows strong homology (77.38-92.89 %) with Prx 1 proteins from other species, including fish, amphibians, and mammals, and it is most closely related to rainbow smelt Prx 1. RuPrx 1 mRNA was ubiquitously detected in all tested tissues and its expression was comparatively high in the brain, intestine, kidney, liver, ovary, stomach, and testis. Expression of RuPrx 1 mRNA in liver peaked 3 h post-infection with Aeromonas hydrophila and decreased 24 h post-infection while the expression in intestine decreased 24 h post-infection. These results suggest that RuPrx 1 is conserved through evolution and may play roles similar to its mammalian counterparts.
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, 216 Gijanghaean-ro, Gijang-eup, Gijang-gun, Busan, 619-705, Republic of Korea.
authors.forename: Hyun Kook
authors.initials: HK
authors.lastname: Cho
authors.forename: Hee Jeong
authors.initials: HJ
authors.lastname: Kong
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Moon
authors.forename: Jin-Do
authors.initials: JD
authors.lastname: Kim
authors.forename: Dong-Gyun
authors.initials: DG
authors.lastname: Kim
authors.forename: Woo-Jin
authors.initials: WJ
authors.lastname: Kim
authors.forename: Bo-Hye
authors.initials: BH
authors.lastname: Nam
authors.forename: Young-Ok
authors.initials: YO
authors.lastname: Kim
authors.forename: Hyung Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: Cheul Min
authors.initials: CM
authors.lastname: An
authors.forename: Bong-Seok
authors.initials: BS
authors.lastname: Kim
mesh_terms: D016980:Aeromonas hydrophila; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003530:Cyprinidae / Q000145:classification / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry; D005393:Fish Diseases / Q000235:genetics / Q000382:microbiology; D015870:Gene Expression; D020869:Gene Expression Profiling; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D054464:Peroxiredoxins / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D054464:Peroxiredoxins