title: Nuclear ribosomal RNA genes: ITS region.
abstract: Despite possible drawbacks (intraspecific polymorphisms and possible fungal contamination), sequencing of the ribosomal RNA gene ITS region remains one of the most popular nuclear sequences used for plant taxonomy and phylogeny. A protocol for PCR amplification and sequencing of this region using universal plant primers is provided.
authors.affiliation: UMR C53 PVBMT Université de la Réunion - Cirad, Université de la Réunion, Ile de la Réunion, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Besse
mesh_terms: D002467:Cell Nucleus / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012335:RNA, Ribosomal / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D021903:DNA, Ribosomal Spacer; D012335:RNA, Ribosomal