title: Increase of Myoglobin in Rat Gastrocnemius Muscles with Immobilization-induced Atrophy.
abstract: [Purpose] Atrophy is a common phenomenon caused by prolonged muscle disuse associated with bed-rest, aging, and immobilization. However, changes in the expression of atrophy-related myoglobin are still poorly understood. In the present study, we examined whether or not myoglobin expression is altered in the gastrocnemius muscles of rats after seven days of cast immobilization. [Methods] We conducted a protein expression and high-resolution differential proteomic analysis using, two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight/time-of-flight mass spectrometry, and western blotting. [Results] The density and expression of myoglobin increased significantly more in atrophic gastrocnemius muscle strips than they did in the control group. [Conclusion] The results suggest that cast immobilization-induced atrophy may be related to changes in the expression of myoglobin in rat gastrocnemius muscles.
authors.affiliation: Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
authors.forename: Jeong-Uk
authors.initials: JU
authors.lastname: Lee
authors.affiliation: Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
authors.forename: Ju-Hyun
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
authors.forename: Mee-Young
authors.initials: MY
authors.lastname: Kim
authors.affiliation: Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
authors.forename: Lim-Kyu
authors.initials: LK
authors.lastname: Lee
authors.affiliation: Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
authors.forename: Seung-Min
authors.initials: SM
authors.lastname: Yang
authors.affiliation: Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
authors.forename: Hye-Joo
authors.initials: HJ
authors.lastname: Jeon
authors.affiliation: Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
authors.forename: Won-Deok
authors.initials: WD
authors.lastname: Lee
authors.affiliation: Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
authors.forename: Ji-Woong
authors.initials: JW
authors.lastname: Noh
authors.affiliation: Department of Combative Martial Arts Training, College of Martial Arts, Yongin University, Republic of Korea.
authors.forename: Tae-Hyun
authors.initials: TH
authors.lastname: Lee
authors.affiliation: Taekwondo Instructor Education, College of Martial Arts, Yongin University, Republic of Korea.
authors.forename: Taek-Yong
authors.initials: TY
authors.lastname: Kwak
authors.affiliation: Department of Physiology, School of Medicine, Institute of Functional Genomics, Konkuk University, Republic of Korea.
authors.forename: Bokyung
authors.initials: B
authors.lastname: Kim
authors.affiliation: Department of Physical Therapy, College of Public Health and Welfare, Yongin University, Republic of Korea.
authors.forename: Junghwan
authors.initials: J
authors.lastname: Kim
keywords: Atrophy; Gastrocnemius muscle; Myoglobin