title: EphB4 tyrosine kinase stimulation inhibits growth of MDA-MB-231 breast cancer cells in a dose and time dependent manner.
abstract: BACKGROUND
  EphB4 receptor tyrosine kinase is of diagnostic and therapeutic value due to its overexpression in breast tumors. Dual functions of tumor promotion and suppression have been reported for this receptor based on presence or absence of its ligand. To elucidate such discrepancy, we aimed to determine the effect of time- and dose-dependent stimulation of EphB4 on viability and invasion of breast cancer cells via recombinant ephrinB2-Fc.
  
  
  METHODS
  Cells were seeded into multiwell plates and were stimulated by various concentrations of preclustered ephrinB2-Fc. Cell viability was measured on days 3 and 6 following treatment using alamar-blue when cells were in different states of confluence.
  
  
  RESULTS
  Stimulation of cells with ephrinB2 did not pose any significant effect on cell viability before reaching confluence, while inhibition of cell growth was detected after 6 days when cells were in postconfluent state following a dose-dependent manner. EphrinB2 treatment did not affect tubular formation and invasion on matrigel.
  
  
  CONCLUSIONS
  This study showed that EphB4 can differentially inhibit cells at post confluent state and that presence of ligand manifests growth-inhibitory properties of EphB4 receptor. It is concluded that growth inhibition has occurred possibly due to long treatment with ligand, a process which leads to receptor downregulation.
authors.forename: Farnaz
authors.initials: F
authors.lastname: Barneh
authors.forename: Mona
authors.initials: M
authors.lastname: Moshayedi
authors.forename: Hamid
authors.initials: H
authors.lastname: Mirmohammadsadeghi
authors.forename: Shaghayegh
authors.initials: S
authors.lastname: Haghjooy-Javanmard
authors.forename: Ali Mohammad
authors.initials: AM
authors.lastname: Sabzghabaee
authors.forename: Shirinsadat
authors.initials: S
authors.lastname: Badri
mesh_terms: D001943:Breast Neoplasms; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation; D002470:Cell Survival / Q000187:drug effects; D004789:Enzyme Activation; D036388:Ephrin-B2 / Q000494:pharmacology*; D005260:Female; D006801:Humans; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D036224:Receptor, EphB4 / Q000819:agonists / Q000378:metabolism*
chemical_list: D036388:Ephrin-B2; D036224:Receptor, EphB4