title: [Rapid determination of total residues of ribavirin and its metabolites in chicken and chicken products by ultra-performance hydrophilic interaction chromatography-tandem mass spectrometry].
abstract: An analytical method was developed for the determination of total residues of ribavirin and its phosphorylated metabolites in chicken and its products by hydrophilic interaction chromatography-tandem mass spectrometry. The samples were extracted with acetonitrile containing 1% (v/v) acetic acid under ultrasonic condition and then enzymatically hydrolysed with acid phosphatase at 37 degrees C. After liposoluble substances being removed with hexane, C18 and PSA dispersion solid phase extraction (DSPE) was introduced to cleanup procedures. The separation was performed on an ultra-performance hydrophilic interaction chromatographic (HILIC) amide column under a gradient elution using acetonitrile and 0.1% formic acid. The analytes were detected in the positive electrospray ionization and multi-reaction monitoring (MRM) mode. In the range of 1-200 microg/kg, the correlation coefficient was greater than 0.999. The limit of detection (LOD, S/N > or = 3) was 1 microg/kg and the limit of quantification (LOQ, S/N > or = 10) was 5 microg/kg. The recoveries of ribavirin spiked at three levels ranged from 67.8% to 112.7% with the relative standard deviations (RSDs) of 6.1%-13.6%. The results of the determination of ribavirin in various real samples showed that the method is simple, rapid, accurate and suitable for the determination of total residues of ribavirin and its metabolites in chicken and its products.
authors.affiliation: Henan Entry-Exit Inspection and Quarantine Bureau, Zhengzhou 450003, China.
authors.forename: Weixia
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Henan Entry-Exit Inspection and Quarantine Bureau, Zhengzhou 450003, China.
authors.forename: Jizhou
authors.initials: J
authors.lastname: Yang
authors.affiliation: Henan Entry-Exit Inspection and Quarantine Bureau, Zhengzhou 450003, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Yuan
authors.affiliation: Henan Entry-Exit Inspection and Quarantine Bureau, Zhengzhou 450003, China.
authors.forename: Zhuanlian
authors.initials: Z
authors.lastname: Sun
authors.affiliation: College of Chemistry and Molecular Engineering of Zhengzhou University, Zhengzhou 450042, China.
authors.forename: Caijuan
authors.initials: C
authors.lastname: Wang
authors.affiliation: Henan Entry-Exit Inspection and Quarantine Bureau, Zhengzhou 450003, China.
authors.forename: Wuyong
authors.initials: W
authors.lastname: Sun
authors.affiliation: College of Chemistry and Molecular Engineering of Zhengzhou University, Zhengzhou 450042, China.
authors.forename: Shusheng
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D057927:Hydrophobic and Hydrophilic Interactions; D008460:Meat / Q000032:analysis*; D012254:Ribavirin / Q000032:analysis*; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry
chemical_list: D012254:Ribavirin