title: [Cloning and characterization of an HOG1 MAPK homologous gene CgHOG1 from Candida glycerinogenes].
abstract: OBJECTIVE
  This study was aimed to obtain a key gene of osmo-adaptation and glycerol synthesis regulation in Candida glycerinogenes, namely mitogen-activated protein kinase HOG1 gene (CgHOG1), and to verify its function of osmo-regulation.
  
  
  METHODS
  The gene CgHOG1 was amplified by Degenerate PCR and Self-Formed Adaptor PCR from the C. glycerinogenes genome and the bioinformatic analysis of CgHOG1 gene was conducted. The CgHOG1 gene was transformed in Saccharomyces cerevisiae hog1delta null mutant and its salt tolerance characteristics was investigated.
  
  
  RESULTS
  The gene CgHOG1 encoded a protein of 387 amino acids with an open reading frame of 1164 bp and the amino acid sequence showed 86% identity to Hog1 p of Ogataea parapolymorpha. Heterologous expression of CgHOG1 gene in S. cerevisiae W303 hog1 delta null mutant showed an increase in salt tolerance and glycerol production compared to S. cerevisiae W303 hog1 delta null mutant.
  
  
  CONCLUSIONS
  The CgHOG1 obtained in this study is a novel HOG1 gene from C. glycerinogenes, which plays an essential role in the yeast hyperosmotic response and glycerol synthesis. We supplied a new gene for the osmo-adaptation mechanism in C. glycerinogenes and molecular modification of the salt-tolerant and drought-resistant crops.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China. chenying071776@163.com
authors.forename: Chenying
authors.initials: C
authors.lastname: Wang
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhuge
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Fang
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zong
authors.affiliation: School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Song
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhuge
mesh_terms: D000595:Amino Acid Sequence; D002175:Candida / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D020929:Mitogen-Activated Protein Kinase Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016415:Sequence Alignment
chemical_list: D005656:Fungal Proteins; D020929:Mitogen-Activated Protein Kinase Kinases; D005990:Glycerol