title: Separation and quantification of neoagaro-oligosaccharides.
abstract: Oligosaccharides were obtained from agar by enzymatic hydrolysis. Activated carbon adsorption separation was used to extract oligosaccharides, and gel chromatography separation was applied to further purify oligosaccharides. The result showed that activated carbon adsorption could remove the most salt impurities, and gel column chromatography could give the separation of the two kinds of oligosaccharides. ESI-MS, (13)C-NMR revealed that the molecular weight (Mw) of two oligosaccharides were 630 and 936, which were identified as neoagarotetraose and neoagarohexaose respectively.
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310032 China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Mei
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310032 China.
authors.forename: Jinting
authors.initials: J
authors.lastname: Shao
authors.affiliation: Food Research Program, Agriculture and Agri-Food Canada, Guelph, N1G 5C9 ON Canada.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310032 China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310032 China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yi
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310032 China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Ying
keywords: Gel chromatography; Neoagaro-oligosaccharides; Separation; Structure Identification