title: Development of porcine respiratory and reproductive syndrome virus replicon vector for foot-and-mouth disease vaccine.
abstract: OBJECTIVE
  Foot-and-mouth disease (FMD) is an economically important global animal disease. To control FMD virus (FMDV) outbreaks, a lot of different novel approaches have been attempted. In this study, we proposed a novel porcine reproductive and respiratory syndrome virus (PRRSV) as a replicon vector to express FMDV structural protein.
  
  
  METHODS
  PRRSV infectious clone (PRRSVK418DM) was used to develop an expression vector through the reverse genetic manipulation of PRRSV; FMDVP12A3C gene of serotype O was synthesized and used for an antigen. MARC-145 cells (African green monkey kidney epithelial cell line) were used for electroporation mediated transfection. The transfection or the expression of P12A3C and N protein of PRRSV was analyzed by either replicon containing PRRSV alone or by co-infection of helper PRRSV.
  
  
  RESULTS
  We constructed PRRSVK418DM replicon vector containing FMDVP12A3C, and genome sequences were confirmed by subsequent sequence analysis. In vitro expression of P12A3C and PRRSV N protein was confirmed by immunofluorescence antibody assay using antibodies specific for PRRSV N protein (anti-PRRSV N MAb), FMDV-VP1 (anti-VP1 MAb).
  
  
  CONCLUSIONS
  The results indicate that PRRSV replicon vector can be a promising novel vector system to control FMDV and useful for vaccine development in the future.
authors.affiliation: College of Veterinary Medicine and Veterinary Science Research institute, Konkuk University, Seoul, Korea.
authors.forename: Subbiah
authors.initials: S
authors.lastname: Jeeva
authors.affiliation: College of Veterinary Medicine and Veterinary Science Research institute, Konkuk University, Seoul, Korea.
authors.forename: Jung-Ah
authors.initials: JA
authors.lastname: Lee
authors.affiliation: College of Veterinary Medicine and Veterinary Science Research institute, Konkuk University, Seoul, Korea.
authors.forename: Seung-Yong
authors.initials: SY
authors.lastname: Park
authors.affiliation: College of Veterinary Medicine and Veterinary Science Research institute, Konkuk University, Seoul, Korea.
authors.forename: Chang-Seon
authors.initials: CS
authors.lastname: Song
authors.affiliation: College of Veterinary Medicine and Veterinary Science Research institute, Konkuk University, Seoul, Korea.
authors.forename: In-Soo
authors.initials: IS
authors.lastname: Choi
authors.affiliation: College of Veterinary Medicine and Veterinary Science Research institute, Konkuk University, Seoul, Korea.
authors.forename: Joong-Bok
authors.initials: JB
authors.lastname: Lee
keywords: FMDV; Genetic vectors; PRRSV; Replicon