title: [Injury repairing and magnetic resonance tracing of transplanted bone marrow stromal cells in rat liver with hepatic fibrosis].
abstract: OBJECTIVE
  To explore the recurring patterns of migration and distribution of transplanted bone marrow stromal cells (BMSCs) in rat model with hepatic fibrosis and the feasibility of magnetic resonance (MR) tracing.
  
  
  METHODS
  BMSCs labeled with 5-bromodeoxyuridine (Brdu) and super para-magnetic iron oxide (SPIO) were transplanted into rat model with hepatic fibrosis via portal vein. MR scan was performed at Hour 2, Day 3, Day 7 and Week 2 post-transplantation to analyze the hepatic features of MR signal intensity and pathohistology of BMSCs.
  
  
  RESULTS
  Multiple hypo-intense lesions appeared in hepatic hilar region at Hour 2 and became smaller with the elapsing time. Hemosiderin and Brdu immunohistochemical stains showed that positive cells were found in portal vein of hepatic porta at Hour 2, small branches of portal vein, sinus hepaticus and around central veins of hepatic lobules at Day 3 and liver parenchyma (esp. in area of lesion) at Day 7 and Week 2. Some of transplanted BMSCs were tightly connected with liver cell to form liver cell cord. The signal intensity changes of MRI corresponded to histological findings at different timepoints.
  
  
  CONCLUSIONS
  The transplanted BMSCs are gradually scattered in whole liver (esp. in lesion area) so that it may help to repair hepatic lesions. And the recurring patterns of MR signal intensity changes reflected the condition of distribution, immigration and differentiation of transplanted cells.
authors.affiliation: Department of Radiology, Second Affiliated Hospital, Soochow University, Suzhou 215004, China.
authors.forename: Guo-hua
authors.initials: GH
authors.lastname: Fan
authors.affiliation: Department of Radiology, Second Affiliated Hospital, Soochow University, Suzhou 215004, China.
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Gong
authors.affiliation: Department of Radiology, Second Affiliated Hospital, Soochow University, Suzhou 215004, China.
authors.forename: Jun-kang
authors.initials: JK
authors.lastname: Shen
authors.affiliation: Department of Radiology, Second Affiliated Hospital, Soochow University, Suzhou 215004, China.
authors.forename: Cai-yuan
authors.initials: CY
authors.lastname: Zhang
authors.affiliation: Department of Radiology, Second Affiliated Hospital, Soochow University, Suzhou 215004, China.
authors.forename: Xiao-qiu
authors.initials: XQ
authors.lastname: Xu
authors.affiliation: Department of Radiology, Second Affiliated Hospital, Soochow University, Suzhou 215004, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Radiology, Second Affiliated Hospital, Soochow University, Suzhou 215004, China.
authors.forename: Ze-yang
authors.initials: ZY
authors.lastname: Yu
authors.affiliation: Department of Radiology, Second Affiliated Hospital, Soochow University, Suzhou 215004, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Second Affiliated Hospital, Soochow University, Suzhou 215004,Email: wuhaorong@vip.sina.c.
authors.forename: Hao-rong
authors.initials: HR
authors.lastname: Wu
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D008099:Liver / Q000473:pathology*; D008106:Liver Cirrhosis, Experimental / Q000473:pathology* / Q000628:therapy*; D008279:Magnetic Resonance Imaging; D051381:Rats; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells / Q000637:transplantation*