title: [Comparison of three different methods in transfection of plasmid pGPU6/GFP/Neo to chicken embryo fibroblasts].
abstract: To compare three different methods in transfection of plasmid pGPU6/GFP/Neo into chicken embryo fibroblast.
  
  
  
  Different doses (1.25 μl,2 μl,2.5 μl) of Lipofec-tamin2000, Gbfectene-Elite and HilyMax were used to transfect 1 μg plasmid pGPU6/GFP/Neo. The transfection efficiency was observed by the fluorescence and the cell viabilities were measured.
  
  
  
  The transfection efficiency of HilyMax was significant higher than that of Lipofectamin2000 and Gbfectene-Elite (86.85%±2.32% compared with 48.33%±3.24% and compared with 37.35%±5.41%; F=18.882, P<0.05). The transfection efficiency of 2.5 μl HilyMax was highest(90.53%±1.15%). The cell viability of Lipofectamin2000 was significantly lower than that of HilyMax and Gbfectene-Elite(65.76%±5.78% compared with 89.54%±0.86% and compared with 82.45%±3.56%;F=90.676, P<0.05).
  
  
  
  HilyMax has the highest transfection efficiency and the lowest cell toxicity in transfection of plasmid pGPU6/GFP/Neo into chicken embryo fibroblast, with the optimal ratio of 2.5 μl HilyMax:1 μg plasmid.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, 310016, China.
authors.forename: Xing
authors.initials: X
authors.lastname: He
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Shao
authors.forename: Ying
authors.initials: Y
authors.lastname: Hu
authors.forename: Zichun
authors.initials: Z
authors.lastname: Gu
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: Jingjing
authors.initials: J
authors.lastname: Ma
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Lan
mesh_terms: D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D005347:Fibroblasts / Q000166:cytology; D049452:Green Fluorescent Proteins / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D014162:Transfection / Q000379:methods*
chemical_list: D049452:Green Fluorescent Proteins