title: Effects of compositional changes of AFEX-treated and H-AFEX-treated corn stover on enzymatic digestibility.
abstract: Corn stover is one of the main agricultural residues being considered as a cellulosic ethanol feedstock. This work evaluated the effectiveness of AFEX™(1) pretreatment for converting corn stover to fermentable sugars, both with and without pre-soaking in hydrogen peroxide. The compositional changes and enzymatic digestibility of AFEX-treated and H-AFEX-treated biomass were investigated. Results showed that most of the polysaccharides remained intact following each of these two methods. Compared with AFEX pretreatment, the H-AFEX process enhanced delignification and enzymatic hydrolysis yields of both glucose and xylose. The maximum glucan and xylan digestibility of H-AFEX process were 87.78% and 90.64%, respectively, and were obtained using 0.7 (w/w) water loading, 1.0 (w/w) ammonia loading, 0.5 (w/w) 30wt.% hydrogen peroxide loading, and 130°C for 10min. The results of the present work show that H-AFEX is a feasible pretreatment to improve the enzymatic saccharification of corn stover for bioethanol production.
authors.affiliation: College of Engineering, Nanjing Agricultural University, Nanjing, Jiangsu 210031, China; National Engineering Research Center for Wood-based Resource Utilization, School of Engineering, Zhejiang A&F University, Linan, Zhejiang 311300, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhao
authors.affiliation: College of Engineering, Nanjing Agricultural University, Nanjing, Jiangsu 210031, China. Electronic address: wmding@njau.edu.cn.
authors.forename: Weimin
authors.initials: W
authors.lastname: Ding
authors.affiliation: National Engineering Research Center for Wood-based Resource Utilization, School of Engineering, Zhejiang A&F University, Linan, Zhejiang 311300, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.affiliation: National Engineering Research Center for Wood-based Resource Utilization, School of Engineering, Zhejiang A&F University, Linan, Zhejiang 311300, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Cheng
authors.affiliation: National Engineering Research Center for Wood-based Resource Utilization, School of Engineering, Zhejiang A&F University, Linan, Zhejiang 311300, China. Electronic address: shawqj@gmail.com.
authors.forename: Qianjun
authors.initials: Q
authors.lastname: Shao
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D056804:Biofuels; D001709:Biotechnology / Q000379:methods*; D000431:Ethanol; D005936:Glucans / Q000378:metabolism; D006861:Hydrogen Peroxide; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D014990:Xylans / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry*
chemical_list: D056804:Biofuels; D005936:Glucans; D014990:Xylans; D000431:Ethanol; D000641:Ammonia; D008031:Lignin; D006861:Hydrogen Peroxide
keywords: Ammonia fiber expansion pretreatment (AFEX); Bioethanol; Enzymatic hydrolysis; Hydrogen peroxide; Lignocellulosic biomass