title: Expression and Antibody Preparation of GP5a Gene of Porcine Reproductive and Respiratory Syndrome Virus.
abstract: Porcine reproductive and respiratory syndrome (PRRS) is considered one of the most important infectious diseases to affect the swine industry and characterized by reproductive failure in late term gestation in sows and respiratory disease in pigs of all ages. The GP5a gene, encoding RNA-dependent RNA polymerase, is generally regarded as fairly conserved when compared to other viral proteins. It plays an important role in the process of duplication and transcription carried out by Porcine reproductive and respiratory syndrome virus (PRRSV). We firstly expressed and purified the GP5a protein of PRRSV. This provides a good method for the purification of expressed proteins and the preparation of the corresponding antibodies.
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642 Guangdong China.
authors.forename: Chunya
authors.initials: C
authors.lastname: Wei
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642 Guangdong China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Huang
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642 Guangdong China.
authors.forename: Long
authors.initials: L
authors.lastname: Sun
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642 Guangdong China.
authors.forename: Jiexiong
authors.initials: J
authors.lastname: Xie
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642 Guangdong China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642 Guangdong China.
authors.forename: Minze
authors.initials: M
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642 Guangdong China ; Beijing Dabeinong Swine Technology Co., Ltd, 14F Zhongguancun Building No.27 Zhongguancun street, Beijing, 100080 China ; Guangzhou Lizhi Agriculture Co., Ltd, Guangzhou, China.
authors.forename: Chaoyi
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Beijing Dabeinong Swine Technology Co., Ltd, 14F Zhongguancun Building No.27 Zhongguancun street, Beijing, 100080 China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Qi
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642 Guangdong China.
authors.forename: Wenbao
authors.initials: W
authors.lastname: Qi
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642 Guangdong China.
authors.forename: Zhangyong
authors.initials: Z
authors.lastname: Ning
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642 Guangdong China.
authors.forename: Liguo
authors.initials: L
authors.lastname: Yuan
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642 Guangdong China.
authors.forename: Heng
authors.initials: H
authors.lastname: Wang
authors.affiliation: Guangzhou Lizhi Agriculture Co., Ltd, Guangzhou, China.
authors.forename: Liangquan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642 Guangdong China.
authors.forename: Guihong
authors.initials: G
authors.lastname: Zhang
keywords: Expression; GP5a; Porcine reproductive and respiratory syndrome virus; Purification