title: Characteristics of a highly thermostable neutral protease produced fromBacillus stearothermophilus.
abstract: A highly thermostable neutral protease was found in culture filtrates ofBacillus stearothermophilus. The optimum reaction pH and temperature of this protease were 6.0 and 60°C, respectively, and 90% activity remained even after heat treatment at 90°C for 30 min. The protease was markedly inactivated by diisopropyl fluorophosphate, but EDTA and iodoacetic acid hardly affected it. The neutral protease therefore could be defined as a highly thermostable, neutral(-serine) protease.
authors.affiliation: Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, 812, Fukuoka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Fujio
authors.forename: S
authors.initials: S
authors.lastname: Kume