title: [Levo-L-methyl tryptophan induces immune imbalance at maternal-fetal interface in mice].
abstract: OBJECTIVE
  To investigate which one, 1-methyl-L-tryptophan (1-L-MT) or 1-methyl-D-tryptophan (1-D-MT), induces immune imbalance at maternal-fetal interface in mice.
  
  
  METHODS
  BALB/c female mice are mated with C57BL/6J male mice, and since pregnancy day 6.5, the pregnant mice were given respectively 1-L-MT, 1-D-MT and organic solvents for 10 d. All the pregnant mice were executed at pregnancy day 16.5. Indoleamine 2, 3-dioxygenase (IDO) activity in the placenta tissue was detected using high performance liquid chromatography (HPLC), IFN-γ/IL-4 ratio in the decidual tissue was determined by flow cytometry and pregnancy outcome was compared among groups.
  
  
  RESULTS
  IDO activity of placenta tissues in 1-L-MT group was obviously lower than that in 1-D-MT group and the control group, and no statistical difference was found between 1-D-MT group and the control group; IFN-γ/IL-4 ratio of decidual tissues in 1-L-MT group was higher than that in 1-D-MT group and the control group, and there was no statistical difference between 1-D-MT group and the control group; the quantity and weight of fetal mice in 1-L-MT group were significantly lower than those in 1-D-MT group and the control group, and no statistical difference between 1-D-MT group and the control group.
  
  
  CONCLUSIONS
  1-L-MT but not 1-D-MT induces immune imbalance at maternal-fetal interface in mice by inhibiting activity of IDO.
authors.affiliation: Department of Gynecology and Obstetrics, Shenzhen Maternal and Child Care Hospital Affiliated to South Medical University, Shenzhen 518028, China.
authors.forename: Huanhua
authors.initials: H
authors.lastname: Wang
authors.forename: Chenhong
authors.initials: C
authors.lastname: Wang
mesh_terms: D000818:Animals; D005260:Female; D005333:Fetus; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009035:Mothers; D010920:Placenta / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism; D011247:Pregnancy; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology
chemical_list: D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D015847:Interleukin-4; C028129:tryptophan methyl ester; D007371:Interferon-gamma; D014364:Tryptophan