title: Non-invasive airway health assessment: synchrotron imaging reveals effects of rehydrating treatments on mucociliary transit in-vivo.
abstract: To determine the efficacy of potential cystic fibrosis (CF) therapies we have developed a novel mucociliary transit (MCT) measurement that uses synchrotron phase contrast X-ray imaging (PCXI) to non-invasively measure the transit rate of individual micron-sized particles deposited into the airways of live mice. The aim of this study was to image changes in MCT produced by a rehydrating treatment based on hypertonic saline (HS), a current CF clinical treatment. Live mice received HS containing a long acting epithelial sodium channel blocker (P308); isotonic saline; or no treatment, using a nebuliser integrated within a small-animal ventilator circuit. Marker particle motion was tracked for 20 minutes using PCXI. There were statistically significant increases in MCT in the isotonic and HS-P308 groups. The ability to quantify in vivo changes in MCT may have utility in pre-clinical research studies designed to bring new genetic and pharmaceutical treatments for respiratory diseases into clinical trials.
authors.affiliation: 1] Respiratory and Sleep Medicine, Women's and Children's Hospital, 72 King William Road, North Adelaide, SA, 5006, Australia [2] Centre for Stem Cell Research, University of Adelaide, SA, 5001, Australia [3] School of Paediatrics and Reproductive Health, University of Adelaide, SA, 5001, Australia.
authors.forename: Martin
authors.initials: M
authors.lastname: Donnelley
authors.affiliation: School of Physics, Monash University, Clayton, Vic, 3800, Australia.
authors.forename: Kaye S
authors.initials: KS
authors.lastname: Morgan
authors.affiliation: 1] School of Physics, Monash University, Clayton, Vic, 3800, Australia [2] Monash Biomedical Imaging, Monash University, Clayton, Vic, 3800, Australia [3] Imaging and Medical Beamline, Australian Synchrotron, Clayton, Vic, 3800, Australia.
authors.forename: Karen K W
authors.initials: KK
authors.lastname: Siu
authors.affiliation: 1] Respiratory and Sleep Medicine, Women's and Children's Hospital, 72 King William Road, North Adelaide, SA, 5006, Australia [2] Centre for Stem Cell Research, University of Adelaide, SA, 5001, Australia [3] School of Paediatrics and Reproductive Health, University of Adelaide, SA, 5001, Australia.
authors.forename: Nigel R
authors.initials: NR
authors.lastname: Farrow
authors.affiliation: Mechanical and Aerospace Engineering, Monash University, Clayton, Vic, 3800, Australia.
authors.forename: Charlene S
authors.initials: CS
authors.lastname: Stahr
authors.affiliation: CF/Pulmonary Research & Treatment Center, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
authors.forename: Richard C
authors.initials: RC
authors.lastname: Boucher
authors.affiliation: Mechanical and Aerospace Engineering, Monash University, Clayton, Vic, 3800, Australia.
authors.forename: Andreas
authors.initials: A
authors.lastname: Fouras
authors.affiliation: 1] Respiratory and Sleep Medicine, Women's and Children's Hospital, 72 King William Road, North Adelaide, SA, 5006, Australia [2] Centre for Stem Cell Research, University of Adelaide, SA, 5001, Australia [3] School of Paediatrics and Reproductive Health, University of Adelaide, SA, 5001, Australia.
authors.forename: David W
authors.initials: DW
authors.lastname: Parsons
mesh_terms: D000818:Animals; D003550:Cystic Fibrosis / Q000175:diagnosis / Q000000981:diagnostic imaging*; D003952:Diagnostic Imaging / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D010316:Particle Size; D011877:Radionuclide Imaging; D012137:Respiratory System / Q000000981:diagnostic imaging*; D012462:Saline Solution, Hypertonic / Q000008:administration & dosage*; D017356:Synchrotrons; D014965:X-Rays
chemical_list: D012462:Saline Solution, Hypertonic