title: Transmission and evolution of hepatitis C virus in HCV seroconverters in HIV infected subjects.
abstract: HIV/HCV co-infection provides a model to determine the role of immunity on HCV transmission and evolution. In this study HCV transmission and evolution were evaluated in 6 HCV seroconverters in HIV-infected subjects with a wide range of CD4 cell count. The HCV envelope E1/E2 sequences were analyzed for transmission bottleneck, viral diversity/divergence, immune pressure, and mutations of HLA class I/II restricted epitopes. HCV infection started with transmission bottleneck in all HIV-infected individuals. During the 1.0-2.0 years of infection there was a shift of viral quasispecies in majority of the subjects from one to next visit. However, HCV diversity, divergence, mutations in HLA class I/II restricted and virus neutralizing epitopes were similar in all subjects regardless of CD4 cell count at the time of HCV infection. Our results suggest that HCV transmission and evolution in HIV-infected subjects may not be influenced by host CD4 cell count at the time of infection.
authors.affiliation: Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA 15261, United States.
authors.forename: Chengli
authors.initials: C
authors.lastname: Shen
authors.affiliation: Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA 15261, United States. Electronic address: pgupta1@pitt.edu.
authors.forename: Phalguni
authors.initials: P
authors.lastname: Gupta
authors.affiliation: Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA 15261, United States.
authors.forename: Xiaochuan
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA 15261, United States.
authors.forename: Anwesha
authors.initials: A
authors.lastname: Sanyal
authors.affiliation: Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA 15261, United States.
authors.forename: Charles
authors.initials: C
authors.lastname: Rinaldo
authors.affiliation: Department of Epidemiology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, United States.
authors.forename: Eric
authors.initials: E
authors.lastname: Seaberg
authors.affiliation: Department of Epidemiology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, United States.
authors.forename: Joseph B
authors.initials: JB
authors.lastname: Margolick
authors.affiliation: Department of Epidemiology, UCLA School of Public Health, LA, CA, United States.
authors.forename: Otoniel
authors.initials: O
authors.lastname: Martinez-Maza
authors.affiliation: Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA 15261, United States. Electronic address: cheny@pitt.edu.
authors.forename: Yue
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000328:Adult; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D060085:Coinfection / Q000235:genetics / Q000276:immunology / Q000635:transmission* / Q000821:virology*; D019143:Evolution, Molecular; D005260:Female; D005500:Follow-Up Studies; D015658:HIV Infections / Q000235:genetics / Q000276:immunology / Q000821:virology*; D015497:HIV-1 / Q000502:physiology; D006680:HLA Antigens / Q000235:genetics / Q000276:immunology; D016174:Hepacivirus / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology; D006508:Hepatitis Antibodies / Q000276:immunology; D006526:Hepatitis C / Q000235:genetics / Q000276:immunology / Q000635:transmission* / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: D006680:HLA Antigens; D006508:Hepatitis Antibodies
keywords: CD4 cell count; Evolution; HCV seroconverter; HIV; Transmission