Oral administration of Lactobacillus plantarum strain AYA enhances IgA secretion and provides survival protection against influenza virus infection in mice.
The mucosal immune system provides the first line of defense against inhaled and ingested pathogenic microbacteria and viruses. This defense system, to a large extent, is mediated by the actions of secretory IgA. In this study, we screened 140 strains of lactic acid bacteria for induction of IgA production by murine Peyer's patch cells. We selected one strain and named it Lactobacillus plantarum AYA. We found that L. plantarum AYA-induced production of IL-6 in Peyer's patch dendritic cells, with this production promoting IgA(+) B cells to differentiate into IgA-secreting plasma cells. We also observed that oral administration of L. plantarum AYA in mice caused an increase in IgA production in the small intestine and lung. This production of IgA correlated strongly with protective ability, with the treated mice surviving longer than the control mice after lethal influenza virus infection. Our data therefore reveals a novel immunoregulatory role of the L. plantarum AYA strain which enhances mucosal IgA production and provides protection against respiratory influenza virus infection.
Research Center for Basic Science, Research and Development, Quality Assurance Division, Nisshin Seifun Group Inc., Fujimino-city, Saitama, Japan.
Yosuke
Y
Kikuchi
Research Center for Basic Science, Research and Development, Quality Assurance Division, Nisshin Seifun Group Inc., Fujimino-city, Saitama, Japan.
Ayami
A
Kunitoh-Asari
Research Center for Basic Science, Research and Development, Quality Assurance Division, Nisshin Seifun Group Inc., Fujimino-city, Saitama, Japan.
Katsuyuki
K
Hayakawa
Research Center for Basic Science, Research and Development, Quality Assurance Division, Nisshin Seifun Group Inc., Fujimino-city, Saitama, Japan ; Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka-city, Shizuoka, Japan.
Shinjiro
S
Imai
Yeast Function Development Unit, Oriental Yeast Co., Ltd., Itabashi-ku, Tokyo Japan.
Kenji
K
Kasuya
Laboratory of Yeast and Fermentation Food Division, Oriental Yeast Co., Ltd., Itabashi-ku, Tokyo, Japan.
Kimio
K
Abe
Department of Immunology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan.
Yu
Y
Adachi
Research Center for Basic Science, Research and Development, Quality Assurance Division, Nisshin Seifun Group Inc., Fujimino-city, Saitama, Japan.
Shin-Ichi
S
Fukudome
Department of Immunology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan.
Yoshimasa
Y
Takahashi
Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Satoshi
S
Hachimura
D000284:Administration, Oral; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D007071:Immunoglobulin A, Secretory / Q000276:immunology*; D007421:Intestine, Small / Q000276:immunology; D048191:Lactiplantibacillus plantarum / Q000276:immunology*; D051379:Mice; D009975:Orthomyxoviridae / Q000276:immunology*; D009976:Orthomyxoviridae Infections / Q000235:genetics / Q000276:immunology* / Q000517:prevention & control*; D010581:Peyer's Patches / Q000166:cytology / Q000276:immunology; D019936:Probiotics / Q000008:administration & dosage*
D016207:Cytokines; D007071:Immunoglobulin A, Secretory