Lyso-phosphatidylcholines in Langmuir monolayers--influence of chain length on physicochemical characteristics of single-chained lipids.
Single-chained phospholipids constitute a class of membrane components found in normal cells in relatively low concentration; however, these group of compounds are known owing to their broad physiological activities. Despite that the knowledge concerning fundamental physicochemical properties of lyso-lipids is very limited and in contrast to double-chained phospholipids there is an obvious deficiency of studies focused on correlation between their amphipathic character and film-forming properties with biological activities. In the present paper we have attempted to explain the main issues regarding the characteristics of lyso-PCs in monolayers at the air/aqueous interface. Our results show that all the investigated phospholipids differing in the length of hydrophobic chain: C18lyso-PC, C22lyso-PC and C24lyso-PC form stable Langmuir monolayers of a relatively low degree of condensation. It was found that during compression the investigated monolayers significantly change their organization at the interface which is strongly connected with temperature of the subphase. The application of X-ray reflectivity confirmed that the bulky choline head-groups in molecules of lyso-PCs are strongly penetrated by water molecules, while the hydrophobic chains are significantly tilted from the surface normal. The obtained results show that the phase transitions observed in the course of the registered isotherms result from decrease in immersion of molecules in the subphase as well as from the decrease in hydrating water molecules. On the basis of GIXD experiments it turned out that in the monolayers of C22lyso-PC and C24lyso-PC at higher surface pressures (>20 mN/m) small fractions of periodically ordered phase appear at the interface. For the monolayer of C24lyso-PC in the periodically ordered fraction the untilted (U) to tilted (t) phase transition was found.
Department of Environmental Chemistry, Faculty of Chemistry, Jagiellonian University, Gronostajowa 3, 30-387 Kraków, Poland. Electronic address: flasinsk@chemia.uj.edu.pl.
Michał
M
Flasiński
Department of Physical Chemistry and Electrochemistry, Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland.
Paweł
P
Wydro
Department of Environmental Chemistry, Faculty of Chemistry, Jagiellonian University, Gronostajowa 3, 30-387 Kraków, Poland.
Marcin
M
Broniatowski
D000388:Air / Q000032:analysis; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D008244:Lysophosphatidylcholines / Q000737:chemistry*; D044367:Phase Transition; D053838:Scattering, Small Angle; D013329:Structure-Activity Relationship; D013499:Surface Properties; D013696:Temperature; D013816:Thermodynamics; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction
D008244:Lysophosphatidylcholines; D014867:Water
Brewster angle microscopy; Grazing incidence X-ray diffraction; Lyso-lipids; Lyso-phosphatidylcholines; Phase transition; X-ray reflectivity