The maternal ITPK1 gene polymorphism is associated with neural tube defects in a high-risk Chinese population.
BACKGROUND
Epidemiological surveys and animal studies have revealed that inositol metabolism is associated with NTDs, but the mechanisms are not clear. Inositol 1,3,4-trisphosphate 5/6-kinase (ITPK1) is a pivotal regulatory enzyme in inositol metabolic pathway. The objective was to assess the potential impact of the maternal ITPK1 genotypes on the inositol parameter and on the NTD risk in a NTD high-risk area in China.


RESULTS
A case-control study of pregnant women affected with NTDs (n = 200) and controls (n = 320) was carried out. 13 tag SNPs of ITPK1 were selected and genotyped by the Sequenom MassArray system. We found that 4 tag SNPs were statistically significant in spina bifida group (P<0.05). MACH was used to impute the un-genotyped SNPs in ITPK1 locus and showed that 3 meaningful SNPs in the non-coding regions were significant. We also predicted the binding capacity of transcription factors in the positive SNPs using the bioinformatics method and found that only rs3783903 was located in the conserved sequence of activator protein-1 (AP-1). To further study the association between biochemical values and genotypes, maternal plasma inositol hexakisphosphate (IP6) levels were also assessed using LC-MS. The maternal plasma IP6 concentrations in the spina bifida subgroup were 7.1% lower than control (136.67 vs. 147.05 ng mL(-1), P<0.05), and significantly lower in rs3783903 GG genotype than others (P<0.05). EMSA showed a different allelic binding capacity of AP-1 in rs3783903, which was affected by an A→G exchange. The RT-PCR suggested the ITPK1 expression was decreased significantly in mutant-type of rs3783903 compared with wild-type in the 60 healthy pregnancies (P<0.05).


CONCLUSIONS
These results suggested that the maternal rs3783903 of ITPK1 might be associated with spina bifida, and the allele G of rs3783903 might affect the binding of AP-1 and the decrease of maternal plasma IP6 concentration in this Chinese population.
Department of Biotechnology, Capital Institute of Pediatrics, Beijing, China.
Zhen
Z
Guan
Department of Biotechnology, Capital Institute of Pediatrics, Beijing, China.
Jianhua
J
Wang
Department of Biotechnology, Capital Institute of Pediatrics, Beijing, China.
Jin
J
Guo
Department of Biotechnology, Capital Institute of Pediatrics, Beijing, China.
Fang
F
Wang
Department of Biotechnology, Capital Institute of Pediatrics, Beijing, China.
Xiuwei
X
Wang
Department of Biotechnology, Capital Institute of Pediatrics, Beijing, China.
Guannan
G
Li
Department of Biotechnology, Capital Institute of Pediatrics, Beijing, China.
Qiu
Q
Xie
Department of Biotechnology, Capital Institute of Pediatrics, Beijing, China.
Xu
X
Han
Department of Biotechnology, Capital Institute of Pediatrics, Beijing, China.
Bo
B
Niu
Beijing Municipal Key Laboratory of Child Development and Nutriomics, Capital Institute of Pediatrics, Beijing, China.
Ting
T
Zhang
D000328:Adult; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D005260:Female; D005838:Genotype; D006801:Humans; D007294:Inositol / Q000235:genetics; D007295:Inositol Phosphates / Q000097:blood; D009436:Neural Tube Defects / Q000097:blood / Q000453:epidemiology / Q000235:genetics*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D012307:Risk Factors; D016135:Spinal Dysraphism / Q000235:genetics; D055815:Young Adult
D007295:Inositol Phosphates; D007294:Inositol; C058097:inositol 1,3,4-trisphosphate; D017853:Phosphotransferases (Alcohol Group Acceptor); C056875:myo-inositol-trisphosphate 6-kinase