Proteomic analyses of baculovirus Anticarsia gemmatalis multiple nucleopolyhedrovirus budded and occluded virus.
Baculoviruses infect insects, producing two distinct phenotypes during the viral life cycle: the budded virus (BV) and the occlusion-derived virus (ODV) for intra- and inter-host spread, respectively. Since the 1980s, several countries have been using Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) as a biological control agent against the velvet bean caterpillar, A. gemmatalis. The genome of AgMNPV isolate 2D (AgMNPV-2D) carries at least 152 potential genes, with 24 that possibly code for structural proteins. Proteomic studies have been carried out on a few baculoviruses, with six ODV and two BV proteomes completed so far. Moreover, there are limited data on virion proteins carried by AgMNPV-2D. Therefore, structural proteins of AgMNPV-2D were analysed by MALDI- quadrupole-TOF and liquid chromatography MS/MS. A total of 44 proteins were associated with the ODV and 33 with the BV of AgMNPV-2D. Although 38 structural proteins were already known, we found six new proteins in the ODV and seven new proteins carried by the AgMNPV-2D BV. Eleven cellular proteins that were found on several other enveloped viruses were also identified, which are possibly carried with the virion. These findings may provide novel insights into baculovirus biology and their host interaction. Moreover, our data may be helpful in subsequent applied studies aiming to improve AgMNPV use as a biopesticide and a biotechnology tool for gene expression or delivery.
Laboratory of Molecular Evolution and Bioinformatics, Department of Microbiology, Biomedical Sciences Institute - ICB II, University of São Paulo - USP, Brazil.
Carla Torres
CT
Braconi
Cell Biology Department, University of Brasília, Brasília, DF, CEP 70910-970, Brazil.
Daniel Mendes Pereira
DMP
Ardisson-Araújo
Laboratory of Mass Spectrometry, Brazilian Biosciences National Laboratory - LNBio - CNPEM, Campinas - SP, Brazil.
Adriana Franco Paes
AFP
Leme
Laboratory of Molecular Evolution and Bioinformatics, Department of Microbiology, Biomedical Sciences Institute - ICB II, University of São Paulo - USP, Brazil.
Juliana Velasco de Castro
JVC
Oliveira
Laboratory of Mass Spectrometry, Brazilian Biosciences National Laboratory - LNBio - CNPEM, Campinas - SP, Brazil.
Bianca Alves
BA
Pauletti
Insect Virology Laboratory, Entomology and Nematology Department, 970 Natural Area Dr., University of Florida, Gainesville, FL 32611, USA.
Alejandra
A
Garcia-Maruniak
Cell Biology Department, University of Brasília, Brasília, DF, CEP 70910-970, Brazil.
Bergmann Morais
BM
Ribeiro
Insect Virology Laboratory, Entomology and Nematology Department, 970 Natural Area Dr., University of Florida, Gainesville, FL 32611, USA.
James E
JE
Maruniak
Laboratory of Molecular Evolution and Bioinformatics, Department of Microbiology, Biomedical Sciences Institute - ICB II, University of São Paulo - USP, Brazil.
Paolo Marinho de Andrade
PMA
Zanotto
D000818:Animals; D002460:Cell Line; D002853:Chromatography, Liquid; D007181:Inclusion Bodies, Viral; D007915:Lepidoptera; D017924:Nucleopolyhedroviruses / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015678:Viral Structural Proteins / Q000032:analysis*; D057074:Virus Release
D020543:Proteome; D015678:Viral Structural Proteins