Prevalence of pathogenic Yersinia enterocolitica in slaughter-aged pigs during a one-year survey, 2010-2011, France.
The prevalence of pathogenic Yersinia enterocolitica in French slaughter-aged pigs was estimated by sampling 3120 pigs from 96 batches in 16 slaughterhouses from January 2010 to February 2011. Respectively, 36 batches (20 pigs/batch) and 60 batches (40 pigs/batch) were considered during the cold period and the warm period. Tonsils were swabbed before the chilling step. Pathogenic Y. enterocolitica was detected after enrichment in ITC and streaking on CIN and YeCM media. Typical isolates were confirmed as Y. enterocolitica and biotyped by biochemical tests as described in the ISO 10273:2003 method. Of the tested pigs, 13.7% (CI95% [10.1-17.3]) were found positive for pathogenic Y. enterocolitica and 74.3% (CI95% [64.8-83.8]) of the pig batches contained at least one positive pig. The percentage of positive pigs per batch was generally low; 60.3% of positive batches contained fewer than 5 positive pigs. The prevalence of the pathogen at the batch level remained unchanged throughout this one-year study, but the prevalence in pigs was significantly higher during the warm period than during the cold period. Biotype 4 was the most prevalent biotype among the 827 isolated strains (91.9% of the isolates), followed by biotype 3 (7.25% of the isolates). Six isolates were of biotype 5 and one of biotype 2. Biotype 4 was found in all the 16 participating slaughterhouses, biotype 3 in ten slaughterhouses and biotype 5 in four. This study provides valuable recent figures for the prevalence of pathogenic Y. enterocolitica in French pigs. It also highlights the seasonal aspect of the carriage of this pathogen by pigs, a pattern which differs from those in other countries.
ANSES, Ploufragan/Plouzané Laboratory, Hygiene and Quality of Poultry and Pig Products Unit, BP53, F-22440 Ploufragan, France; European University of Brittany, F-35000, France European University of Brittany, France.
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M
Fondrevez
IFIP, La motte au Vicomte, BP 35104, Le Rheu cedex, F-35651, France.
B
B
Minvielle
ANSES, Ploufragan/Plouzané Laboratory, Hygiene and Quality of Poultry and Pig Products Unit, BP53, F-22440 Ploufragan, France; European University of Brittany, F-35000, France European University of Brittany, France.
A
A
Labbé
ANSES, Ploufragan/Plouzané Laboratory, Hygiene and Quality of Poultry and Pig Products Unit, BP53, F-22440 Ploufragan, France; European University of Brittany, F-35000, France European University of Brittany, France.
C
C
Houdayer
ANSES, Ploufragan/Plouzané Laboratory, Swine Epidemiology and Welfare Unit, BP53, F-22440 Ploufragan, France; European University of Brittany, F-35000, France European University of Brittany, France.
N
N
Rose
ANSES, Ploufragan/Plouzané Laboratory, Hygiene and Quality of Poultry and Pig Products Unit, BP53, F-22440 Ploufragan, France; European University of Brittany, F-35000, France European University of Brittany, France.
E
E
Esnault
ANSES, Ploufragan/Plouzané Laboratory, Hygiene and Quality of Poultry and Pig Products Unit, BP53, F-22440 Ploufragan, France; European University of Brittany, F-35000, France European University of Brittany, France. Electronic address: martine.denis@anses.fr.
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M
Denis
D000818:Animals; D005602:France / Q000453:epidemiology; D014066:Palatine Tonsil / Q000382:microbiology; D015995:Prevalence; D012621:Seasons; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000382:microbiology*; D015009:Yersinia Infections / Q000453:epidemiology / Q000662:veterinary*; D015008:Yersinia enterocolitica / Q000302:isolation & purification
Biotype; Prevalence; Slaughter-aged pigs; Yersinia enterocolitica