Beta-glucuronidase and Beta-glucosidase activity in stool specimens of children with inflammatory bowel disease.
The aim of the study was to analyze the differences in the activity of beta-glucuronidase and beta-glucosidase in stool specimens of children with Inflammatory Bowel Diseases (IBD) and healthy subjects. The disease activity was determined according to the PCDAI scale (Crohn disease) and Truelove-Witts scale (Ulcerative colitis). Enzyme activity was determined by spectrophotometry. There was a correlation between the level of beta - glucosidase activity in stool and patient's age in the group of healthy controls, but not in the IBD group. beta-glucosidase activity in IBD and healthy subjects stool specimens did not differ significantly. The activity of beta-glucuronidase in children with IBD was two times lower than in the healthy group and was correlated with age in children with IBD, but not in the group of healthy ones.
Institute of Fermentation Technology and Microbiology, Technical University of Lódi, Poland.
Marta
M
Mroczyńska
Institute of Microecology in Poznań, Poland
Miroslawa
M
Galecka
Institute of Microecology in Poznań, Poland
Patrycja
P
Szachta
Institute of Fermentation Technology and Microbiology, Technical University of Lódi, Poland.
Dorota
D
Kamoda
Institute of Fermentation Technology and Microbiology, Technical University of Lódi, Poland.
Zdzislawa
Z
Libudzisz
First Department of Paediatrics, Department of Paediatric Gastroenterology and Metabolic Diseases, Medical University in Poznań, Poznań.
Dorota
D
Roszak
D000293:Adolescent; D001419:Bacteria / Q000201:enzymology*; D001426:Bacterial Proteins / Q000032:analysis* / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D005243:Feces / Q000737:chemistry / Q000382:microbiology*; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology; D005966:Glucuronidase / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D007223:Infant; D015212:Inflammatory Bowel Diseases / Q000382:microbiology*; D008297:Male; D001617:beta-Glucosidase / Q000032:analysis* / Q000378:metabolism
D001426:Bacterial Proteins; D001617:beta-Glucosidase; D005966:Glucuronidase