Influence of pectins on NADPH oxidase and phagocytic activity of neutrophils during cryopreservation.
NADPH oxidase activation was studied by detecting the reactive oxygen species (ROS) and the dynamics of neutrophil phagocytic activity in blood before freezing and one day after exposure to -20 degrees C in the presence of pectic polysaccharides. Pectins (excluding lemnan) were found to increase the phagocytic activity of neutrophils, promote the formation of ROS in these cells and enhance NADPH oxidase activity in the following order: bergenan, comaruman, lemnan, apiuman, and potamogetonan. Cryoprotective action of lemnan and comaruman was proposed to be related to their unique chemical structures.
Laboratory of Cryophysiology of Blood (T.V. Polezhaeva) of the Physiology Institute at the Komi Scientific Center, Ural Branch of the Russian Academy of Sciences, Komi Republic, Syktyvkar, Russian Federation. ddic@yandex.ru
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Zaitseva
Laboratory of Cryophysiology of Blood (T.V. Polezhaeva) of the Physiology Institute at the Komi Scientific Center, Ural Branch of the Russian Academy of Sciences, Komi Republic, Syktyvkar, Russian Federation.
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Polezhaeva
Laboratory of Cryophysiology of Blood (T.V. Polezhaeva) of the Physiology Institute at the Komi Scientific Center, Ural Branch of the Russian Academy of Sciences, Komi Republic, Syktyvkar, Russian Federation.
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Khudyakov
Laboratory of Cryophysiology of Blood (T.V. Polezhaeva) of the Physiology Institute at the Komi Scientific Center, Ural Branch of the Russian Academy of Sciences, Komi Republic, Syktyvkar, Russian Federation.
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Solomina
Laboratory of Cryophysiology of Blood (T.V. Polezhaeva) of the Physiology Institute at the Komi Scientific Center, Ural Branch of the Russian Academy of Sciences, Komi Republic, Syktyvkar, Russian Federation.
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Laptev
Kirov Research Institute of Haematology and Blood Transfusion, Kirov, Russian Federation.
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Svedentsov
Kirov Research Institute of Haematology and Blood Transfusion, Kirov, Russian Federation.
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Utemov
Kirov Research Institute of Haematology and Blood Transfusion, Kirov, Russian Federation.
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Kostyaev
D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods; D004789:Enzyme Activation; D006801:Humans; D019255:NADPH Oxidases / Q000378:metabolism*; D009504:Neutrophils / Q000166:cytology* / Q000378:metabolism; D010368:Pectins / Q000378:metabolism*; D010587:Phagocytosis; D017382:Reactive Oxygen Species / Q000378:metabolism
D017382:Reactive Oxygen Species; D010368:Pectins; D019255:NADPH Oxidases