A proteomic analysis using an animal model for hyperlipidemia-related erectile dysfunction.
To investigate the pathogenesis of hyperlipidemia-related erectile dysfunction and the effects of DA-8159, a new phosphodiesterase-5 inhibitor, on protein expression, we performed a proteomic analysis of differentially expressed proteins in the corpus cavernosum of hyperlipidemic rats by two-dimensional electrophoresis-mass spectrometry. Rats were fed high-cholesterol diet and treated with 5 mg·kg(-1)·day(-1) DA-8159 concurrently. After 5 months apparent hyperlipidemia and significantly decreased maximal intra-cavernous pressure were observed in the control group with the alteration of 8 proteins, which were restored by DA-8159 treatment. The proteins whose levels decreased >2-fold and attenuated by DA-8159 were determined alcohol dehydrogenase, aldolase A, annexin 1, and tropomyosin-rat, whereas proteins increased>2-fold and recovered by DA-8159 were found to be aldehyde dehydrogenase complex, guanine deaminase, creatine kinase-B, and phosphoglycerate mutase type B subunit.
Ajou University, Yeongtong, Suwon, Gyunggi, Republic of Korea.
Y-G
YG
Hwang
Ajou University, Yeongtong, Suwon, Gyunggi, Republic of Korea.
H
H
Lee
Ajou University, Yeongtong, Suwon, Gyunggi, Republic of Korea.
S
S
Lee
Research Institutes of Dong-A ST Company, Gyunggi, Republic of Korea.
H K
HK
Chung
Research Institutes of Dong-A ST Company, Gyunggi, Republic of Korea.
K K
KK
Kang
Research Institutes of Dong-A ST Company, Gyunggi, Republic of Korea.
S H
SH
Kim
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D007172:Erectile Dysfunction / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology; D006949:Hyperlipidemias / Q000150:complications*; D008297:Male; D010410:Penile Erection; D010413:Penis / Q000187:drug effects / Q000378:metabolism* / Q000503:physiopathology; D058986:Phosphodiesterase 5 Inhibitors / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D011743:Pyrimidines / Q000494:pharmacology; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013449:Sulfonamides / Q000494:pharmacology; D013997:Time Factors; D000096926:Benzenesulfonamides
D015415:Biomarkers; D058986:Phosphodiesterase 5 Inhibitors; D011506:Proteins; D011743:Pyrimidines; D013449:Sulfonamides; C419664:udenafil; D000096926:Benzenesulfonamides