APUM23, a PUF family protein, functions in leaf development and organ polarity in Arabidopsis.
The normal biological function of leaves, such as intercepting light and exchanging gases, relies on proper differentiation of adaxial and abaxial polarity. KANADI (KAN) genes, members of the GARP family, are key regulators of abaxial identity in leaf morphogenesis. This study identified a mutant allele (apum23-3) of APUM23, which encodes a Pumilio/PUF domain protein and acts as an enhancer of the kan mutant. Arabidopsis APUM23 has been shown to function in pre-rRNA processing and play pleiotropic roles in plant development. The apum23-3 mutant also synergistically interacts with other leaf polarity mutants, affects proliferation of division-competent cells, and alters the expression of important leaf polarity genes. These phenotypes show that APUM23 has critical functions in plant development, particularly in polarity formation. The PUF gene family is conserved across kingdoms yet it has not been well characterized in plants. These results illuminating the functions of APUM23 suggest a novel role for PUF genes in Arabidopsis leaf development.
Department of Plant Biology and Pathology, Waksman Institute, Rutgers, State University of New Jersey, 190 Frelinghuysen Road, Piscataway, NJ 08854, USA.
Tengbo
T
Huang
Randall A
RA
Kerstetter
Vivian F
VF
Irish
D000483:Alleles; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002455:Cell Division / Q000235:genetics; D016764:Cell Polarity / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D005816:Genetic Complementation Test; D056626:Inflorescence / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D009154:Mutation; D010641:Phenotype; D018515:Plant Leaves / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D017434:Protein Structure, Tertiary; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D036226:Seedlings / Q000166:cytology / Q000235:genetics / Q000254:growth & development
C000592781:APUM23 protein, Arabidopsis; D029681:Arabidopsis Proteins; D012322:RNA Precursors; D018749:RNA, Plant; D016601:RNA-Binding Proteins
APUM23; Arabidopsis; KANADI; PUF.; leaf development; polarity