BurrH: a new modular DNA binding protein for genome engineering.
The last few years have seen the increasing development of new DNA targeting molecular tools and strategies for precise genome editing. However, opportunities subsist to either improve or expand the current toolbox and further broaden the scope of possible biotechnological applications. Here we report the discovery and the characterization of BurrH, a new modular DNA binding protein from Burkholderia rhizoxinica that is composed of highly polymorphic DNA targeting modules. We also engineered this scaffold to create a new class of designer nucleases that can be used to efficiently induce in vivo targeted mutagenesis and targeted gene insertion at a desired locus.
1] CELLECTIS S.A., 8 Rue de la Croix Jarry, 75013 Paris, France [2].
Alexandre
A
Juillerat
1] CELLECTIS S.A., 8 Rue de la Croix Jarry, 75013 Paris, France [2].
Claudia
C
Bertonati
CELLECTIS S.A., 8 Rue de la Croix Jarry, 75013 Paris, France.
Gwendoline
G
Dubois
CELLECTIS S.A., 8 Rue de la Croix Jarry, 75013 Paris, France.
Valérie
V
Guyot
CELLECTIS S.A., 8 Rue de la Croix Jarry, 75013 Paris, France.
Séverine
S
Thomas
CELLECTIS S.A., 8 Rue de la Croix Jarry, 75013 Paris, France.
Julien
J
Valton
CELLECTIS S.A., 8 Rue de la Croix Jarry, 75013 Paris, France.
Marine
M
Beurdeley
CELLECTIS S.A., 8 Rue de la Croix Jarry, 75013 Paris, France.
George H
GH
Silva
CELLECTIS S.A., 8 Rue de la Croix Jarry, 75013 Paris, France.
Fayza
F
Daboussi
CELLECTIS S.A., 8 Rue de la Croix Jarry, 75013 Paris, France.
Philippe
P
Duchateau
D000595:Amino Acid Sequence; D001483:Base Sequence; D019117:Burkholderia / Q000201:enzymology* / Q000235:genetics; D019295:Computational Biology; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005818:Genetic Engineering / Q000379:methods*; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016335:Zinc Fingers / Q000235:genetics
D004268:DNA-Binding Proteins