Improvements to polar 2-D electrophoresis for proteomic applications.
Recently, we reported a new way of performing 2-DE, called P-dimensional electrophoresis (2-PE). In this approach, the second dimension is achieved in a radial gel which can accommodate up to six 7 cm long IPG strips simultaneously, improving reproducibility and throughput power in respect to 2-DE. Nevertheless, 2-PE was up to now limited to the use of only short strips because of technical difficulties. Here, we describe how to load longer strips (e.g., 18-24 cm) on 2-PE and report some representative images for a qualitative assessment.
Department of Biotechnology, Proteomics and Mass Spectrometry Laboratory, University of Verona, Verona, Italy.
Rita
R
Polati
Daniela
D
Cecconi
Ryan
R
Dosselli
Mattia
M
Zaccarin
Renato
R
Millioni
D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation / Q000379:methods*; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D020615:Stenotrophomonas maltophilia / Q000737:chemistry* / Q000378:metabolism
D001426:Bacterial Proteins