Cotinine concentration in serum correlates with tobacco smoke-induced emphysema in mice.
Secondhand smoke (SHS) has been associated with a variety of adverse health outcomes in nonsmokers, including emphysema (a chronic obstructive pulmonary disease). One way to detect SHS exposure is to measure the concentration of cotinine, the primary metabolite of nicotine, in bodily fluids. We have developed a method for cotinine analysis by combining micellar electrokinetic chromatography with enrichment techniques. We employed the method to measure cotinine concentrations in serum samples of mice exposed to tobacco smoke for 12 or 24 weeks and found that it was 3.1-fold or 4.8-fold higher than those exposed to room air for the same period. Further, we investigated the morphological changes in lungs of mice and observed tobacco smoke induced emphysema. Our results indicate that the method can be used to measure cotinine and there is an association between the serum cotinine concentration and tobacco smoke-induced emphysema in mice.
1] Interdisciplinary Microsystems Group, Department of Mechanical and Aerospace Engineering, University of Florida, PO Box 116250, Gainesville, Florida 32611, USA [2].
Xin
X
Xu
Department of Pharmacology and Toxicology, Medical College of Georgia, Georgia Regents University, Augusta, GA 30912, USA.
Yunchao
Y
Su
1] Interdisciplinary Microsystems Group, Department of Mechanical and Aerospace Engineering, University of Florida, PO Box 116250, Gainesville, Florida 32611, USA [2] J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, PO Box 116131, Gainesville, Florida 32611, USA [3] Department of Chemistry, University of Florida, PO Box 117200, Gainesville, FL 32611, USA.
Z Hugh
ZH
Fan
D000818:Animals; D003367:Cotinine / Q000097:blood*; D004646:Emphysema / Q000097:blood / Q000209:etiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012906:Smoke / Q000009:adverse effects*
D012906:Smoke; D003367:Cotinine