A fiber-optic system for dual-modality photoacoustic microscopy and confocal fluorescence microscopy using miniature components.
Imaging of the cells and microvasculature simultaneously is beneficial to the study of tumor angiogenesis and microenvironments. We designed and built a fiber-optic based photoacoustic microscopy (PAM) and confocal fluorescence microscopy (CFM) dual-modality imaging system. To explore the feasibility of this all-optical device for future endoscopic applications, a microelectromechanical systems (MEMS) scanner, a miniature objective lens, and a small size optical microring resonator as an acoustic detector were employed trying to meet the requirements of miniaturization. Both the lateral resolutions of PAM and CFM were quantified to be 8.8 μm. Axial resolutions of PAM and CFM were experimentally measured to be 19 μm and 53 μm, respectively. The experiments onex vivoanimal bladder tissues demonstrate the good performance of this system in imaging not only microvasculature but also cellular structure, suggesting that this novel imaging technique holds potential for improved diagnosis and guided treatment of bladder cancer.
Department of Radiology, University of Michigan, Ann Arbor, MI 48109.
Sung-Liang
SL
Chen
Department of Radiology, University of Michigan, Ann Arbor, MI 48109.
Zhixing
Z
Xie
Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, MI 48109.
L Jay
LJ
Guo
Department of Radiology, University of Michigan, Ann Arbor, MI 48109.
Xueding
X
Wang
bladder cancer; confocal fluorescence microscopy; endoscopy; microelectromechanical systems; microring resonator; photoacoustic microscopy