Molecular logic gates on DNA origami nanostructures for microRNA diagnostics.
Molecular computing holds great promise for diagnosis and treatment of diseases at the molecular level; nevertheless, designing molecular logic gates to operate programmably and autonomously for molecular diagnostics still remains challenging. We designed logic gates on DNA Origami for microRNA analysis. As a demonstration, two indicators of heart failure, microRNA-21 and microRNA-195, were selected as the logic inputs. The logic gates contain two main modules: computation module and output module, performing in a single DNA Origami nanostructure. The computation module recognizes disease indicators, while the output module display different nanoscale symbols, "+" (positive) or "-" (negative), depending on the computing results. We demonstrated that the molecular logic gates worked well with single and two input combinations.
Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, China.
Dongfang
D
Wang
Yanming
Y
Fu
Juan
J
Yan
Bin
B
Zhao
Bin
B
Dai
Jie
J
Chao
Huajie
H
Liu
Dannong
D
He
Yi
Y
Zhang
Chunhai
C
Fan
Shiping
S
Song
D039301:Computers, Molecular; D004247:DNA / Q000737:chemistry*; D035683:MicroRNAs / Q000032:analysis*; D049329:Nanostructures / Q000737:chemistry*
D035683:MicroRNAs; D004247:DNA