[The new isoform of Oct-1 transcription factor is transcribed from alternative promoter].
The Oct-1 transcription factor belongs to the family of highly conserved POU-domain proteins that participate in regulation of housekeeping and tissue-specific gene expression. Several isoforms of Oct-1 were described previously. We have found the new alternative promoter of Oct-1 gene and the corresponding mRNA Oct-1X. This mRNA encodes the alternative Oct-1 isoform that has truncated N-terminus comparing to isoforms Oct-1A and Oct-1L. We explored the abundance of this isoform in human tissues and cell lines and demonstrated that it is ubiquitously expressed but its expression strongly varies in different tissues. By co-transfection and double luciferase assay we have demonstrated that Oct-1X is the activator of housekeeping (histone H2B) and tissue-specific genes (B29) transcription.
I D
ID
Krylova
T N
TN
Portseva
S G
SG
Georgieva
A G
AG
Stepchenko
E V
EV
Pankratova
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D006657:Histones / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D050811:Octamer Transcription Factor-1 / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger
D006657:Histones; D050811:Octamer Transcription Factor-1; D020033:Protein Isoforms; D012333:RNA, Messenger; D008156:Luciferases