Cloning of Hsp21 gene and its expression in Chinese shrimp Fenneropenaeus chinensis in response to WSSV challenge.
In the present study, a DNA sequence encoding a small heat shock protein gene (FcHsp21) in the Chinese shrimp, Fenneropenaeus chinensis, was cloned, and its expression was analyzed after white spot syndrome virus (WSSV) infection. The FcHsp21 gene contained an open reading frame (ORF) of 555 bp in length, encoding a 184 amino acid protein with a theoretical size of about 21 kDa and a predicted isoelectric point of 5.38. The mRNA of the Hsp21 had a long Poly(A) tail (748 bp) with six polyadenylation signals (AATAA) downstream from the terminator. In addition, the gene contained a relatively long intron (507 bp), which has not been described in shrimps. The intron contained a long compound type microsatellite repeat sequence. The analysis of the phylogenetics revealed that the Hsp21 was highly conserved among the genomes of animals. Our results show that the expression modes of FcHsp21 can be changed by different WSSV infection methods. The expression of FcHsp21 was inhibited by muscle-injecting WSSV, but induced by feeding WSSV.
Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang, 222005, Jiangsu, China.
Huan
H
Gao
Xiaofang
X
Lai
Jie
J
Kong
Weiji
W
Wang
Xianhong
X
Meng
Binlun
B
Yan
Shengli
S
Cai
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D016678:Genome / Q000235:genetics; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000235:genetics* / Q000821:virology*; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic; D046848:White spot syndrome virus 1 / Q000502:physiology*
D018076:DNA, Complementary; D006360:Heat-Shock Proteins