Proteomics changes during the incompatible interaction between cowpea and Colletotrichum gloeosporioides (Penz.) Penz and Sacc.
Anthracnose represents an important disease of cowpea [Vigna unguiculata L. (Walp.)] caused by the hemibiothrophic fungus Colletotrichum gloeosporioides that drastically reduces cowpea field production. In this study we investigated some biochemical aspects underlying the incompatible interaction between a resistant cowpea genotype and C. gloeosporioides using a proteomic approach. Analyses of two-dimensional gel electrophoresis patterns and protein identification indicate C. gloeosporioides infection-dependent cowpea leaf proteome changes associated with metabolism, photosynthesis, response to stress, oxidative burst and scavenging, defense signaling, and pathogenesis-related proteins. Moreover the C. gloeosporioides responsive proteins interaction network in cowpea revealed the interconnected modulation of key cellular processes involving particularly antioxidants proteins, photosynthetic apparatus forming proteins and proteins of the energetic metabolism that interact with each other suggesting that their expression changes are also important for resistance of cowpea to C. gloeosporioides.
Department of Biochemistry and Molecular Biology, Federal University of Ceara, Brazil.
Hudson Fernando N
HF
Moura
Department of Biochemistry and Molecular Biology, Federal University of Ceara, Brazil.
Ilka M
IM
Vasconcelos
Department of Animal Science, Federal University of Ceara, Brazil.
Carlos Eduardo A
CE
Souza
Department of Biochemistry and Molecular Biology, Federal University of Ceara, Brazil.
Fredy D A
FD
Silva
School of Pharmacy, University of Fortaleza, Ceara, Brazil.
Frederico B M B
FB
Moreno
School of Pharmacy, University of Fortaleza, Ceara, Brazil.
Marina D P
MD
Lobo
School of Pharmacy, University of Fortaleza, Ceara, Brazil.
Ana C O
AC
Monteiro-Moreira
Department of Animal Science, Federal University of Ceara, Brazil.
Arlindo A
AA
Moura
Department of Biochemistry and Molecular Biology, Federal University of Ceara, Brazil.
José H
JH
Costa
Department of Biochemistry and Molecular Biology, Federal University of Ceara, Brazil. Electronic address: jtaolive@ufc.br.
José Tadeu A
JT
Oliveira
D020231:Colletotrichum / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D007887:Fabaceae / Q000378:metabolism* / Q000382:microbiology; D054884:Host-Pathogen Interactions; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D020543:Proteome; D021241:Spectrometry, Mass, Electrospray Ionization
D010940:Plant Proteins; D020543:Proteome
Anthracnose; Colletotrichum gloeosporioides; Mass spectrometry; Resistance; Two-dimensional gel electrophoresis; Vigna unguiculata