Detection and characterization of a Trichomonas isolate from a rehabilitated bald eagle (Haliaeetus leucocephalus).
A hatching-year bald eagle (Haliaeetus leucocephalus) was presented for clinical examination after being found unable to fly. Upon admission, routine wet-mount microscopy detected no trichomonads. Five months later, oral cavity inspection found no abnormalities, but the eagle was swabbed for research on trichomonosis in maritime birds. The swab was used to inoculate an InPouch TF culture and trichomonads were visible within 24 hr. Genotyping (ITS) revealed a Trichomonas isolate that was 100% identical to an isolate from a bearded vulture (Gypaetus barbatus) from the Czech Republic. The eagle was treated with metronidazole (50 mg/kg q 12h PO for 5 consecutive days). Following treatment, the eagle was swabbed and the inoculated InPouch TF culture was monitored daily for 1 wk. No trichomonads were observed. Rehabilitation centers interested in surveillance should consider combining the InPouch TF technique with clinical inspection of live birds to confirm trichomonosis and for future research.
Canadian Cooperative Wildlife Health Centre, Department of Pathology & Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE, CIA 4P3, Canada.
Whitney K
WK
Kelly-Clark
Canadian Cooperative Wildlife Health Centre, Department of Pathology & Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE, CIA 4P3, Canada.
Scott
S
McBurney
Canadian Cooperative Wildlife Health Centre, Department of Pathology & Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE, CIA 4P3, Canada.
María J
MJ
Forzán
Department of Companion Animals, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE, CIA 4P3, Canada.
Marion
M
Desmarchelier
Department of Biomedical Sciences, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE, CIA 4P3, Canada.
Spencer J
SJ
Greenwood
D000818:Animals; D001715:Bird Diseases / Q000469:parasitology*; D021901:DNA, Intergenic / Q000235:genetics; D020307:Eagles; D005838:Genotype; D010802:Phylogeny; D014244:Trichomonas / Q000235:genetics / Q000302:isolation & purification*; D014245:Trichomonas Infections / Q000469:parasitology / Q000662:veterinary*
D021901:DNA, Intergenic