High-temperature adapted primitive Protochlamydia found in Acanthamoeba isolated from a hot spring can grow in immortalized human epithelial HEp-2 cells.
To elucidate how ancient pathogenic chlamydiae could overcome temperature barriers to adapt to human cells, we characterized a primitive chlamydia found in HS-T3 amoebae (Acanthamoeba) isolated from a hot spring. Phylogenetic analysis revealed the primitive species to be Protochlamydia. In situ hybridization staining showed broad distribution into the amoebal cytoplasm, which was supported by transmission electron microscopic analysis showing typical chlamydial features, with inclusion bodies including both elementary and reticular bodies. Interestingly, although most amoebae isolated from natural environments show reduced growth at 37°C, the HS-T3 amoebae harbouring the Protochlamydia grew well at body temperature. Although infection with Protochlamydia did not confer temperature tolerance to the C3 amoebae, the number of infectious progenies rapidly increased at 37°C with amoebal lysis. In immortalized human epithelial HEp-2 cells, fluorescence microscopic study revealed atypical inclusion of the Protochlamydia, and quantitative real-time polymerase chain reaction analyses also showed an increase in 16S ribosomal RNA DNA amounts. Together, these results showed that the Protochlamydia found in HS-T3 amoebae isolated from a hot spring successfully adapted to immortalized human HEp-2 cells at 37°C, providing further information on the evolution of ancient Protochlamydia to the present pathogenic chlamydiae.
Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, North-12, West-5, Kita-ku, Sapporo, 060-0812, Japan.
Aya
A
Sampo
Junji
J
Matsuo
Chikayo
C
Yamane
Kenji
K
Yagita
Shinji
S
Nakamura
Natsumi
N
Shouji
Yasuhiro
Y
Hayashi
Tomohiro
T
Yamazaki
Mitsutaka
M
Yoshida
Miho
M
Kobayashi
Kasumi
K
Ishida
Hiroyuki
H
Yamaguchi
D000048:Acanthamoeba / Q000382:microbiology*; D000222:Adaptation, Physiological; D002460:Cell Line; D002695:Chlamydiales / Q000235:genetics / Q000254:growth & development* / Q000648:ultrastructure; D045482:Hot Springs / Q000382:microbiology*; D006358:Hot Temperature; D006801:Humans; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013559:Symbiosis
D012336:RNA, Ribosomal, 16S