Mobile elements and mitochondrial genome expansion in the soil fungus and potato pathogen Rhizoctonia solani AG-3.
The soil fungus Rhizoctonia solani is an economically important pathogen of agricultural and forestry crops. Here, we present the complete sequence and analysis of the mitochondrial genome of R. solani, field isolate Rhs1AP. The genome (235 849 bp) is the largest mitochondrial genome of a filamentous fungus sequenced to date and exhibits a rich accumulation of introns, novel repeat sequences, homing endonuclease genes, and hypothetical genes. Stable secondary structures exhibited by repeat sequences suggest that they comprise functional, possibly catalytic RNA elements. RNA-Seq expression profiling confirmed that the majority of homing endonuclease genes and hypothetical genes are transcriptionally active. Comparative analysis suggests that the mitochondrial genome of R. solani is an example of a dynamic history of expansion in filamentous fungi.
The J. Craig Venter Institute, Rockville, MD, USA.
Liliana
L
Losada
Suman B
SB
Pakala
Natalie D
ND
Fedorova
Vinita
V
Joardar
Svetlana A
SA
Shabalina
Jessica
J
Hostetler
Suchitra M
SM
Pakala
Nikhat
N
Zafar
Elizabeth
E
Thomas
Marianela
M
Rodriguez-Carres
Ralph
R
Dean
Rytas
R
Vilgalys
William C
WC
Nierman
Marc A
MA
Cubeta
D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D019143:Evolution, Molecular; D020869:Gene Expression Profiling; D054629:Genome, Mitochondrial / Q000235:genetics*; D020071:Interspersed Repetitive Sequences; D008969:Molecular Sequence Data; D012232:Rhizoctonia / Q000235:genetics*; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal; D004272:DNA, Mitochondrial
Basidiomycota; homing endonucleases; repetitive elements