A new method for maturity-dependent fractionation of neutrophil progenitors applicable for the study of myelodysplastic syndromes.
We applied our new method, maturity-dependent fractionation of bone marrow-derived neutrophil progenitors, to a study of gene expression profiles during granulopoiesis in myelodysplastic syndromes. CD34+ cells with low density [F1], CD11b-/CD16- [F2], CD11b+/CD16- [F3] and CD11b+/CD16low [F4] with intermediate density, CD11b+/CD16int [F5] and CD11b+/CD16high [F6] with high density were isolated from six patients. Although AML1 and C/EBP-ϵ mRNA peaked at F1 and F4, respectively, in healthy individuals, C/EBP-ϵ was maximized at F2/F3 in all patients, two of whom showed simultaneous peaks of AML1 at F2. Thus, this fractionation is useful to detect mistimed induction of granulopoiesis-regulating genes in myelodysplastic syndromes.
Huiyuan
H
Hu
Department of Pharmacology, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima 960-1295, Japan. yayois@fmu.ac.jp.
Yayoi
Y
Shikama
Tsutomu
T
Shichishima
Kazuhiko
K
Ikeda
Kazuko
K
Akutsu
Tomoyuki
T
Ono
Hideo
H
Kimura
Kazuei
K
Ogawa
Hideyoshi
H
Noji
Yasuchika
Y
Takeishi
Junko
J
Kimura