Effect of simulated extracorporeal circulation and glyceryl-tri-nitrate on leukocyte activation.
OBJECTIVE
During extracorporeal circulation (ECC), a mechanical pump and an oxygenator replace the functions of the heart and lungs. The aim of this study is to test the effect of the nitric oxide donor glyceryl-tri-nitrate on activation markers of the innate immune system during simulated ECC.


METHODS
Whole blood concentrations of selected leukocyte adhesion molecules, complement system components and myeloperoxidase (MPO) were measured in an in vitro system of simulated ECC.


RESULTS
Simulated ECC stimulated the expression of monocyte LPS-receptor CD14 and C3b-receptor CD35. Glyceryl-tri-nitrate significantly reduced the expression of leukocyte Fcγ receptor CD32 over time, compared to control. Simulated ECC increased the concentrations of MPO, terminal complement complex, and complement component C3a. Addition of glyceryl-tri-nitrate did not significantly affect these changes.


CONCLUSIONS
Simulated ECC induces the increased expression of some leukocyte markers. Glyceryl-tri-nitrate addition significantly reduces the expression of some leukocyte activation markers.
Department of Surgical Sciences, Cardiac and Thoracic Surgery, Uppsala University , Uppsala , Sweden.
Vilyam
V
Melki
Lena
L
Douhan Håkansson
Jan W
JW
Borowiec
D015703:Antigens, CD / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D015926:Complement C3a / Q000378:metabolism; D005112:Extracorporeal Circulation / Q000009:adverse effects*; D006801:Humans; D007113:Immunity, Innate / Q000187:drug effects*; D018836:Inflammation Mediators / Q000378:metabolism; D007962:Leukocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D005996:Nitroglycerin / Q000494:pharmacology*; D013997:Time Factors
D015703:Antigens, CD; D015415:Biomarkers; D018836:Inflammation Mediators; D020030:Nitric Oxide Donors; D008315:Malondialdehyde; D015926:Complement C3a; D005996:Nitroglycerin