Lipopolysaccharide impaired the functional activity of endothelial colony-forming cells.
BACKGROUND
Recent studies have shown that endothelial progenitor cells (EPCs) contribute to lung repair after lipopolysaccharide (LPS)-induced lung injury and infusion of LPS decreased early EPCs in human peripheral blood. However, the effects of LPS on endothelial colony-forming cells (ECFCs) remain to be determined.


OBJECTIVE
To investigate possible effects of LPS on the functional activity of ECFCs.


METHODS
ECFCs were isolated from human umbilical cord blood and characterized. ECFCs at passages 3-5 were treated for 24 h with either LPS or vehicle control. Their viability, migration and in vitro vasculogenesis activity were assayed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, modified Boyden chamber and in vitro angiogenesis assays, respectively. ECFC adhesion was assessed by replating cells on fibronectin-coated dishes and subsequent counting of adherent cells.


RESULTS
Incubation with LPS dose-dependently inhibited the viable, migratory, adhesive and in vitro vasculogenesis capacity of ECFCs.


CONCLUSIONS
LPS impaired the functional activity of ECFCs.
Department of Cardiology, First Affiliated Hospital, Shantou University Medical College, Shantou, PR China.
Min
M
Yu
Qingying
Q
Zhang
Qian
Q
Liu
Jing
J
Sun
Guibao
G
Yang
Guoning
G
Zeng
Xuerui
X
Tan
D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects*; D006801:Humans; D008070:Lipopolysaccharides / Q000633:toxicity*; D018919:Neovascularization, Physiologic / Q000187:drug effects
D008070:Lipopolysaccharides