A multilayer tissue engineered meniscus substitute.
Various methods have been tried to treat the main meniscus problem, meniscal tears, for which we believe tissue engineering could be a viable solution. In this study, a three dimensional, collagen-based meniscus substitute was prepared by tissue engineering using human fibrochondrocytes and a collagen based-scaffold. This construct was made with 3 different collagen-based foams interspaced with two electrospun nano/microfibrous mats. The top layer was made of collagen type I-chondroitin sulfate-hyaluronic acid (Coll-CS-HA), and the middle and the bottom layers were made of only collagen type I with different porosities and thus with different mechanical properties. The mats of aligned fibers were a blend of collagen type I and poly(L-lactic acid-co-glycolic acid) (PLGA). After seeding with human fibrochondrocytes, cell attachment, proliferation, and production of extracellular matrix and glucoseaminoglycan were studied. Cell seeding had a positive effect on the compressive properties of foams and the 3D construct. The 3D construct with all its 5 layers had better mechanical properties than the individual foams.
Department of Biotechnology, METU, 06800, Ankara, Turkey.
Albana Ndreu
AN
Halili
Nesrin
N
Hasirci
Vasif
V
Hasirci
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001696:Biomechanical Phenomena; D002448:Cell Adhesion; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000502:physiology / Q000637:transplantation; D002809:Chondroitin Sulfates / Q000737:chemistry; D024042:Collagen Type I / Q000737:chemistry; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry; D007718:Knee Injuries / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D019344:Lactic Acid / Q000737:chemistry; D008422:Materials Testing; D008592:Menisci, Tibial / Q000473:pathology / Q000503:physiopathology; D008968:Molecular Conformation; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D000070600:Tibial Meniscus Injuries; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing / Q000502:physiology
D001672:Biocompatible Materials; D024042:Collagen Type I; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates