Effect of Abnormal Savda Munziq on hypertrophic scar formation in a rabbit ear model.
OBJECTIVE
To investigate whether administrating Abnormal Savda Munziq (ASMq), a traditional Uighur herbal preparation used for the prevention or treatment of diseases, affects hypertrophic scar (HTS) formation by using an established rabbit ear model.


METHODS
The HTS rabbit model was created by circular fullthickness skin excisions on both ears of rabbits. Twenty rabbits were randomized into four groups, with 5 rabbits and 60 wounds in each group. Group A was the control group, treated with normal saline daily. Groups B, C, and D were the treatment groups at three different doses of ASMq (400, 800, and 1200 mg/kg body weight, respectively, daily, by gastrogavage). Twenty wounds were randomly chosen from each group on the 40th day after treatment and specimen were examined. Scar elevation index (SEI) was analyzed with histological assessment, and ultrastructure analysis was analyzed with a transmission electron microscopy.


RESULTS
Groups B, C, and D demonstrated significant reductions in SEI as compared with the control group at 35.9% (P=0.0212), 48.2% (P=0.0108), and 52.7% (P=0.0103), respectively in a dose-response manner. SEI was lowered in Group D compared with Group B with a significant difference (P=0.015). However, there were no significant differences between Groups B and C, or between Groups C and D. Histological analysis showed that highdose ASMq (1200 mg/kg) could enhance the softening of HTS of rabbit ears and increase the compliance as shown in general. Ultrastructure analysis showed that with increased ASMq dose, the fibroblasts, pro-collagen, collagen, endoplasmic reticulum and ribosomes were reduced gradually.


CONCLUSIONS
Orally administered ASMq significantly reduces the severity of HTS in the rabbit ear model. The findings of this study may have clinical implications on the management of human HTS.
Department of Plastic Surgery of the First Teaching Hospital, Xinjiang Medical University, Urumqi, 830054, China.
Hu-jun
HJ
Wang
Wei-cheng
WC
Gao
Shao-lin
SL
Ma
D000818:Animals; D017439:Cicatrix, Hypertrophic / Q000188:drug therapy* / Q000473:pathology; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D004423:Ear / Q000473:pathology*; D005260:Female; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D011817:Rabbits; D014945:Wound Healing / Q000187:drug effects
D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; C000600923:abnormal Savda Munziq