Regulation of the expression of OPN mRNA in the rat as an experimental model of renal stone disease.
We have sequenced a cDNA of calcium oxalate urinary stone protein extracted with EDTA. cDNA sequences showed complete homology between urinary stone protein and human osteopotine (OPN, bone sialoprotein 1). In this study, we investigated the expression of OPN mRNA in rat kidney serving as experimental models for several conditions that are considered to be risk factors in human renal stone formation. In the renal stone formation model, the expression of OPN mRNA in the distal convoluted tubule of the kidney was enhanced compared with the control which was found sporadically positive by in situ hybridization. By Northern blot analysis, the expression of OPN mRNA was increased in pyelonephritis and hydronephrosis models compared with the control, but no changes were observed in dietary-acid or base-loading models. The expression of OPN mRNA was markedly inhibited in the renal stone formation model by concomitant administration of estradiol and/or progesterone.
N
N
Amasaki
T
T
Yamate
T
T
Umekawa
Y
Y
Ishikawa
H
H
Kajikawa
T
T
Kurita
D000818:Animals; D002129:Calcium Oxalate / Q000652:urine; D004195:Disease Models, Animal; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D006801:Humans; D006869:Hydronephrosis / Q000235:genetics* / Q000652:urine; D007684:Kidney Tubules / Q000502:physiology; D008297:Male; D053495:Osteopontin / Q000235:genetics* / Q000378:metabolism; D010052:Ovariectomy; D011374:Progesterone / Q000494:pharmacology; D011704:Pyelonephritis / Q000235:genetics* / Q000652:urine; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D052878:Urolithiasis / Q000235:genetics* / Q000652:urine
D004967:Estrogens; D012333:RNA, Messenger; C506897:Spp1 protein, rat; D053495:Osteopontin; D002129:Calcium Oxalate; D011374:Progesterone