Alterations in red blood cells and plasma properties after acute single bout of exercise.
The aim of this study was to investigate alterations in haemoglobin conformation and parameters related to oxidative stress in whole erythrocytes, membranes, and plasma after a single bout of exercise in a group of young untrained men. Venous blood samples from eleven healthy young untrained males (age = 22 ± 2 years, BMI = 23 ± 2.5 kg/m(2)) were taken from the antecubital vein before an incremental cycling exercise test, immediately after exercise, and 1 hour after exercise. Individual heart rate response to this exercise was 195 ± 12 beats/min and the maximum wattage was 292 ± 27 W. Immediately after exercise, significant increase in standard parameters (haemoglobin, haematocrit, lactate levels, and plasma volume) of blood was observed as well as plasma antioxidant capacity one hour after exercise. Reversible conformational changes in haemoglobin, measured using a maleimide spin label, were found immediately following exercise. The concentration of ascorbic acid inside erythrocytes significantly decreased after exercise. A significant decline in membrane thiols was observed one hour after exercise, but simultaneously an increase in plasma thiols immediately after and 1 h after exercise was also observed. This study shows that a single bout of exercise can lead to mobilization of defensive antioxidant systems in blood against oxidative stress in young untrained men.
Department of Molecular Biophysics, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
Krzysztof
K
Gwozdzinski
Department of Thermobiology, University of Lodz, 90-236 Lodz, Poland.
Anna
A
Pieniazek
Department of Molecular Biophysics, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
Joanna
J
Brzeszczynska
Department of Molecular Biophysics, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
Sabina
S
Tabaczar
Department of Sport Medicine, Medical University of Lodz, 90-647 Lodz, Poland.
Anna
A
Jegier
D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000378:metabolism*; D015444:Exercise / Q000502:physiology*; D006400:Hematocrit; D006801:Humans; D019344:Lactic Acid / Q000097:blood; D008297:Male; D010949:Plasma / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000378:metabolism
D000975:Antioxidants; D013438:Sulfhydryl Compounds; D019344:Lactic Acid