Effect of betulin-containing extract from birch tree bark on α-amylase activity in vitro and on weight gain of broiler chickens in vivo.
In vitro effect of betulin-containing extract from Betula pendula Roth. bark on alpha-amylase activity was studied, the kinetic mechanism of interaction was proposed and in vivo effect of betulin-containing extract on weight gain and meat quality of broiler chickens was evaluated. The highest level of inhibitory activity (20%) was detected in extract concentration of 1,000 mg/L. Increased extract concentration did not lead to increased enzyme inhibition. Using Dixon and Cornish-Bowden coordinates, the competitive mechanism of inhibition was demonstrated. Calculated kinetic parameters were: Km equal to 0.6 mg/mL, Vmax equal to 2.6 and 2.1 mM/min from Lineweaver-Burk and Dixon coordinates, respectively and Ki equal to 3,670 ± 230 mg/mL. The partial inhibition of enzyme indicates the existence of low concentration of active inhibitory form, which reaches saturation level with increased extract concentration in applied suspension. Therefore, Ki has an apparent constant character. This partial inhibition of amylase activity observed in in vitro assay did not affect weight gain and meat quality of broiler chickens during in vivo assay. Rather, the tendency to increase the weight of edible parts and muscles compared to diet without additive suggests that the extract may be a potential food additive in poultry farming. Additionally, it could be a source for further pharmaceutical and pharmacological research.
Nanobioscience group, Chemistry School, Autonomous University of Coahuila, Blvd. V. Carranza e Ing. J. Cardenas V, Saltillo, Coahuila, Mexico, 25280, anna_ilina@hotmail.com.
Anna
A
Ilyina
Roberto
R
Arredondo-Valdés
Salavat
S
Farkhutdinov
Elda Patricia
EP
Segura-Ceniceros
José Luis
JL
Martínez-Hernández
Radik
R
Zaynullin
Rayhana
R
Kunakova
D000818:Animals; D029662:Betula / Q000737:chemistry*; D002645:Chickens; D004032:Diet; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D008460:Meat / Q000032:analysis*; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D014315:Triterpenes / Q000494:pharmacology*; D015430:Weight Gain / Q000187:drug effects*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*; D000094062:Betulinic Acid
D004791:Enzyme Inhibitors; D010936:Plant Extracts; D014315:Triterpenes; D000516:alpha-Amylases; C002503:betulin; D000094062:Betulinic Acid