Inductively coupled plasma mass spectrometry-based method for the specific quantification of sulfenic acid in peptides and proteins.
A robust ICPMS-based method is introduced to obtain relative and absolute quantification of sulfenic acid (SA) in peptides and proteins. A new metal-containing reagent (Ln-DOTA-Dimedone) devised to react specifically with SA has been developed. The lanthanide-containing metal-coded affinity tag (Ln-MeCAT) was used to quantify thiol residues. We presented two approaches which allow the parallel and consecutive determination of SA and thiols in peptide and protein samples. The high sensitivity, structure-independent signal, and multiplexing capabilities of ICPMS together with the specificity of Ln-DOTA-Dimedone and Ln-MeCAT toward sulfenic acid and thiol residues, respectively, allow the characterization of various biological states and offer closer insight onto thiol-sulphenic acid equilibria which are involved in intracellular redox-mediated events altering structure and function of proteins in important diseases.
Humboldt-Universitaet zu Berlin , Laboratory of Analytical and Environmental Chemistry, Department of Chemistry, Brook-Taylor Strasse 2, 12489, Berlin, Germany.
Ahmed H
AH
El-Khatib
Diego
D
Esteban-Fernández
Michael W
MW
Linscheid
D013058:Mass Spectrometry / Q000379:methods*; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D017433:Protein Structure, Secondary; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013054:Spectrophotometry, Atomic / Q000379:methods; D013434:Sulfenic Acids / Q000032:analysis*
D010455:Peptides; D011506:Proteins; D013434:Sulfenic Acids