Effect of Biodentine™ on the proliferation, migration and adhesion of human dental pulp stem cells.
OBJECTIVE
To investigate the proliferative, migratory and adhesion effect of Biodentine™, a new tricalcium silicate cement formulation, on the human dental pulp stem cells (hDPSCs).


METHODS
The cell cultures of hDPSCs obtained from impacted third molars were treated with Biodentine™ extract at four different concentrations: Biodentine™ 0.02mg/ml (BD 0.02), Biodentine™ 0.2mg/ml (BD 0.2), Biodentine™ 2mg/ml (BD 2) and Biodentine™ 20mg/ml (BD 20). Human dental pulp stem cells proliferation was evaluated by MTT (3-(4,5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide) and BrdU (5-bromo-2'-deoxyuridine) viability analysis at different times. Migration was investigated by microphotographs of wound healing and transwell migration assays. Adhesion assay was performed as well in presence of BD 0.2, BD 2 and blank control, while qRT-PCR (quantitative real-time reverse-transcriptase polymerase chain) was used for further analysis of the mRNA expression of chemokine and adhesion molecules in hDPSCs.


RESULTS
Biodentine™ significantly increased proliferation of stem cells at BD 0.2 and BD 2 concentrations while decreased significantly at higher concentration of BD 20. BD 0.2 concentration had a statistically significant increased migration and adhesion abilities. In addition, qRT-PCR results showed that BD 0.2 could have effect on the mRNA expression of chemokines and adhesion molecules in human dental pulp stem cells.


CONCLUSIONS
The data imply that Biodentine™ is a bioactive and biocompatible material capable of enhancing hDPSCs proliferation, migration and adhesion abilities.


CONCLUSIONS
Biodentine™ when placed in direct contact with the pulp during pulp exposure can positively influence healing by enhancing the proliferation, migration and adhesion of human dental pulp stem cells.
Department of Operative Dentistry & Endodontics, Fourth Military Medical University, Xian, China.
Zhirong
Z
Luo
Department of VIP Dental Care, Fourth Military Medical University, Xian, China.
Dongmei
D
Li
Department of Endodontics, University of Pennsylvania, Philadelphia, USA.
Meetu R
MR
Kohli
Department of Operative Dentistry & Endodontics, Fourth Military Medical University, Xian, China.
Qing
Q
Yu
Department of Endodontics, University of Pennsylvania, Philadelphia, USA.
Syngcuk
S
Kim
Department of Operative Dentistry & Endodontics, Fourth Military Medical University, Xian, China. Electronic address: hewenxi@fmmu.edu.cn.
Wen-Xi
WX
He
D000293:Adolescent; D000328:Adult; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000494:pharmacology; D001973:Bromodeoxyuridine; D017610:Calcium Compounds / Q000008:administration & dosage / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000032:analysis; D054377:Chemokine CXCL12 / Q000032:analysis; D004396:Coloring Agents; D003738:Dental Cements / Q000494:pharmacology*; D003782:Dental Pulp / Q000166:cytology* / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000032:analysis; D006801:Humans; D019012:Integrin beta1 / Q000032:analysis; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D008422:Materials Testing; D019718:Receptors, CXCR4 / Q000032:analysis; D017640:Silicates / Q000008:administration & dosage / Q000494:pharmacology*; D013234:Stem Cells / Q000187:drug effects*; D013778:Tetrazolium Salts; D013844:Thiazoles; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis; D055815:Young Adult
D001672:Biocompatible Materials; C111835:CCL2 protein, human; C515255:CXCL12 protein, human; C527276:CXCR4 protein, human; D017610:Calcium Compounds; D018932:Chemokine CCL2; D054377:Chemokine CXCL12; D004396:Coloring Agents; D003738:Dental Cements; D019012:Integrin beta1; D019718:Receptors, CXCR4; D017640:Silicates; D013778:Tetrazolium Salts; D013844:Thiazoles; D019010:Vascular Cell Adhesion Molecule-1; D016222:Fibroblast Growth Factor 2; D018799:Intercellular Adhesion Molecule-1; C506393:tricalcium silicate; C022616:thiazolyl blue; D001973:Bromodeoxyuridine
Biodentine™; Biosilicate cement; Cell adhesion; Cell migration; Cell proliferation; hDPSCs