AG-NGS: a powerful and user-friendly computing application for the semi-automated preparation of next-generation sequencing libraries using open liquid handling platforms.
Next-generation sequencing (NGS) is becoming one of the most widely used technologies in the field of genomics. Library preparation is one of the most critical, hands-on, and time-consuming steps in the NGS workflow. Each library must be prepared in an independent well, increasing the number of hours required for a sequencing run and the risk of human-introduced error. Automation of library preparation is the best option to avoid these problems. With this in mind, we have developed automatic genomics NGS (AG-NGS), a computing application that allows an open liquid handling platform to be transformed into a library preparation station without losing the potential of an open platform. Implementation of AG-NGS does not require programming experience, and the application has also been designed to minimize implementation costs. Automated library preparation with AG-NGS generated high-quality libraries from different samples, demonstrating its efficiency, and all quality control parameters fell within the range of optimal values.
Spanish National Center for Cardiovascular Research (CNIC), Madrid, Spain.
Sergio
S
Callejas
Spanish National Center for Cardiovascular Research (CNIC), Madrid, Spain.
Rebeca
R
Álvarez
Spanish National Center for Cardiovascular Research (CNIC), Madrid, Spain.
Alberto
A
Benguria
Spanish National Center for Cardiovascular Research (CNIC), Madrid, Spain.
Ana
A
Dopazo
D001331:Automation; D023281:Genomics; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D011786:Quality Control; D012984:Software
automation; liquid handling robot; next-generation sequencing; sample preparation; software