The prevalence of badnaviruses in West African yams (Dioscorea cayenensis-rotundata) and evidence of endogenous pararetrovirus sequences in their genomes.
Yam (Dioscorea spp.) is an important vegetatively-propagated staple crop in West Africa. Viruses are pervasive in yam worldwide, decreasing growth and yield, as well as hindering the international movement of germplasm. Badnaviruses have been reported to be the most prevalent in yam, and genomes of some other badnaviruses are known to be integrated in their host plant species. However, it was not clear if a similar scenario occurs in Dioscorea yam. This study was conducted to verify the prevalence of badnaviruses, and determine if badnavirus genomes are integrated in the yam genome. Leaf samples (n=58) representing eight species of yam from global yam collections kept at CIRAD, France, and 127 samples of D. rotundata breeding lines (n=112) and landraces (n=15) at IITA, Nigeria, were screened using generic badnavirus PCR primers. Positive amplification of an expected ca. 579bp fragment, corresponding to a partial RT-RNaseH region, was detected in 47 (81%) of 58 samples analysed from CIRAD collections, and 100% of the 127 IITA D. rotundata samples. All the D. cayenensis and D. rotundata samples from the CIRAD and IITA collections tested PCR-positive, and sequencing of a selection of the PCR products confirmed they were typical of the genus Badnavirus. A comparison of serological and nucleic acid techniques was used to investigate whether the PCR-positives were sequences amplified from badnavirus particles or putative endogenous badnavirus sequences in the yam genome. Protein A sandwich-enzyme-linked immunosorbent assay (PAS-ELISA) with badnavirus polyclonal antisera detected cross-reacting viral particles in only 60% (92 of 153) of the CIRAD collection samples analysed, in contrast to the aforementioned 81% by PCR. Immunosorbent electron microscopy (ISEM) of virus preparations of a select set of 16 samples, representing different combinations of positive and negative PCR and PAS-ELISA results, identified bacilliform particles in 11 of these samples. Three PCR-positive yam samples from Burkina Faso (cv. Pilimpikou) were identified in which no viral particles were detected by either PAS-ELISA or ISEM. Southern hybridisation results using a yam badnavirus RT-RNaseH sequence (Gn155Dr) as probe, supported a lack of badnavirus particles in the cv. Pilimpikou and identified their equivalent sequences to be of plant genome origin. Probe Gn155Dr, however, hybridised to viral particles and plant genomic DNA in three D. rotundata samples from Guinea. These results represent the first data demonstrating the presence of integrated sequences of badnaviruses in yam. The implications of this for virus-indexing, breeding and multiplication of seed yams are discussed.
Natural Resources Institute, University of Greenwich, Central Avenue, Chatham Maritime, Kent ME4 4TB, UK; CIRAD, UMR BGPI, F-34098 Montpellier, France. Electronic address: s.e.seal@gre.ac.uk.
Susan
S
Seal
Natural Resources Institute, University of Greenwich, Central Avenue, Chatham Maritime, Kent ME4 4TB, UK.
Aliyu
A
Turaki
CIRAD, UMR BGPI, F-34098 Montpellier, France.
Emmanuelle
E
Muller
International Institute of Tropical Agriculture (IITA), Oyo Road PMB 5320, Ibadan, Nigeria.
P Lava
PL
Kumar
Natural Resources Institute, University of Greenwich, Central Avenue, Chatham Maritime, Kent ME4 4TB, UK.
Lawrence
L
Kenyon
CIRAD, UMR BGPI, F-34098 Montpellier, France.
Denis
D
Filloux
CIRAD, UMR BGPI, F-34098 Montpellier, France.
Serge
S
Galzi
International Institute of Tropical Agriculture (IITA), Oyo Road PMB 5320, Ibadan, Nigeria.
Antonio
A
Lopez-Montes
CIRAD, UMR BGPI, F-34098 Montpellier, France.
Marie-Line
ML
Iskra-Caruana
D000354:Africa, Western; D019195:Badnavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004279:DNA, Viral / Q000235:genetics*; D027742:Dioscorea / Q000235:genetics / Q000821:virology*; D019143:Evolution, Molecular; D014644:Genetic Variation; D018745:Genome, Plant; D016679:Genome, Viral; D054884:Host-Pathogen Interactions; D010802:Phylogeny; D058974:Phylogeography; D010935:Plant Diseases / Q000235:genetics / Q000821:virology*; D018515:Plant Leaves / Q000235:genetics / Q000821:virology; D016662:Virus Integration
D004279:DNA, Viral
Badnavirus; Dioscorea yam; ELISA; Endogenous pararetroviruses; Immunosorbent electron microscopy; PCR