miR-20a promotes prostate cancer invasion and migration through targeting ABL2.
The aberrant expression of microRNAs (miRNAs) has been found in various types of cancer. The present study found miR-20a was significantly up-regulated in prostate cancer compared with normal prostate tissues. Patients with a higher miR-20a expression had a Gleason score of 7-10 and shorter survival time. The transwell and wound healing assays revealed that blocking expression of miR-20a by miR-20a ASO suppresses the invasion and migration of PC-3 and DU145 cells in vitro and also inhibits tumor growth in vivo. Furthermore, we identified miR-20a directly targets the ABL family non-receptor tyrosine kinases ABL2 and negatively regulates the phosphorylation of its downstream gene p190RhoGAP. Knockdown of ABL2 promoted cell invasion and migration and we identified miR-20a-induced cell invasion and migration can be rescued by ABL2. In conclusion, our findings show that miR-20a significantly contributes to the progression of prostate cancer by targeting ABL2.
Pingjing Hospital, Logistics University of the Chinese People's Armed Police Forces, Tianjin, China.
Xiao-Fei
XF
Qiang
Zheng-Wei
ZW
Zhang
Qian
Q
Liu
Nan
N
Sun
Liang-Liang
LL
Pan
Jing
J
Shen
Tong
T
Li
Chen
C
Yun
Hui
H
Li
Li-Hua
LH
Shi
D020413:3' Untranslated Regions / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation; D020690:GTPase-Activating Proteins / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness / Q000235:genetics*; D010766:Phosphorylation; D011467:Prostate / Q000166:cytology; D011471:Prostatic Neoplasms / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis / Q000235:genetics*; D016315:Proto-Oncogene Proteins c-abl / Q000096:biosynthesis / Q000235:genetics; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering; D015854:Up-Regulation
D020413:3' Untranslated Regions; C513043:ARHGAP5 protein, human; D020690:GTPase-Activating Proteins; C534451:MIRN20a microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C426236:ARG tyrosine kinase; D011505:Protein-Tyrosine Kinases; D016315:Proto-Oncogene Proteins c-abl
ABL2; INVASION; MIGRATION; PROSTATE CANCER; miR-20a; p190RhoGAP