Report into a discordant result at D16S539 between SGM Plus® and PowerPlex® ESI 16 kits in a criminal case sample and implications for the UK National DNA Database upgrade.
Upon re-testing of a DNA extract as part of a defence examination, a discordant result was observed at D16S539. Further STR testing and DNA sequencing of the sample identified the cause as a primer binding site mutation which was shown to be a previously unreported SNP. The testing results obtained in this case are considered in light of the current ongoing Multiplex Upgrade Project in the UK and the likely increase in discordant results that may be observed once different next generation kits are introduced.
King's College London, Department of Forensic and Analytical Science, Franklin Wilkins Building, 150 Stamford Street, London SE1 9NH, UK. Electronic address: andrew.revoir@kcl.ac.uk.
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Revoir
King's College London, Department of Forensic and Analytical Science, Franklin Wilkins Building, 150 Stamford Street, London SE1 9NH, UK.
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Ballard
King's College London, Department of Forensic and Analytical Science, Franklin Wilkins Building, 150 Stamford Street, London SE1 9NH, UK.
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Syndercombe Court
D016172:DNA Fingerprinting / Q000295:instrumentation*; D030541:Databases, Genetic; D006801:Humans; D018895:Microsatellite Repeats; D060885:Multiplex Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D16S539; Discordance; Multiplex Upgrade Project; Next generation multiplex kits; UK National DNA database