EAPB0503, a novel imidazoquinoxaline derivative, inhibits growth and induces apoptosis in chronic myeloid leukemia cells.
Imatinib, the first-generation tyrosine kinase inhibitor, revolutionized the therapeutic management of chronic myeloid leukemia (CML) and is highly effective in inducing remissions and prolonging the survival of CML patients. However, one-third of patients develop intolerance or resistance to treatment, and CML stem cells remain insensitive to this therapy, leading almost inevitably to relapse upon treatment discontinuation. Imidazoquinoxalines are imiquimod derivatives that induce growth inhibition and induction of caspase-dependent apoptosis in melanoma and T-cell lymphoma cells. We investigated the effects of EAPB0203 and EAPB0503, two novel imidazoquinoxaline derivatives, on human CML cell lines and showed that they induced a dose-dependent and time-dependent cell growth inhibition. EAPB0503 proved more potent and induced a specific cell cycle arrest in mitosis in CML cells and direct activation of apoptosis as evidenced by increased pre-G0 population, breakdown of mitochondrial membrane potential, PARP cleavage, and DNA breakage. Interestingly, EAPB0503 decreased BCR-ABL oncoprotein levels. The combination of EAPB0503 with imatinib synergized to inhibit the proliferation of CML cells, and most importantly, EABP0503 inhibited the proliferation of imatinib-resistant CML cells, offering promising therapeutic modalities that would circumvent resistance to tyrosine kinase inhibitors and improve the prognosis of CML.
Departments of aAnatomy, Cell Biology and Physiological Sciences bInternal Medicine, Faculty of Medicine, American University of Beirut, Beirut, Lebanon cPharmacochemistry and Biomolecules Labs, Faculty of Pharmacy, Montpellier 1 University, Montpellier dHematology Lab and Blood Diseases Department, Bordeaux University Hospital, Victor Segalen University, Bordeaux, France.
Jessica
J
Saliba
Carine
C
Deleuze-Masquéfa
Ahmad
A
Iskandarani
Rabab
R
El Eit
Raed
R
Hmadi
François-Xavier
FX
Mahon
Ali
A
Bazarbachi
Pierre-Antoine
PA
Bonnet
Rihab
R
Nasr
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001549:Benzamides / Q000494:pharmacology; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D019008:Drug Resistance, Neoplasm; D004357:Drug Synergism; D006801:Humans; D000068877:Imatinib Mesylate; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000473:pathology*; D008938:Mitosis / Q000187:drug effects; D010879:Piperazines / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D011810:Quinoxalines / Q000494:pharmacology*
D000970:Antineoplastic Agents; D001549:Benzamides; C528810:EAPB0203; C555838:EAPB0503; D010879:Piperazines; D011743:Pyrimidines; D011810:Quinoxalines; D000068877:Imatinib Mesylate