Maternal high-fat diet affects Msi/Notch/Hes signaling in neural stem cells of offspring mice.
Numerous research have begun to reveal the importance of maternal nutrition in offspring brain development. Particularly, the maternal obesity or exposure to high-fat diet has been strongly suggested to exert irreversible impact on the structure and function of offspring's brain. However, it remains obscure about whether neonatal neural stem cells (NSCs) in offspring's brain are susceptible to maternal exposure to high-fat diet. Here we focused on the alternation in the Notch signaling in NSCs derived from neonatal mice, which had been given birth by female mice with a high-fat diet and found that, in fact, the high-fat diet administration imposed effects on not only maternal mice, indicated by the accumulation of viscera fat as well as the increase in body weight and serum total cholesterol, but also NSCs in the offspring's brain, where significant increase was observed in the expression of genes, either downstream of Notch signaling or regulating this pathway, which have been shown essential for the maturation of NSCs. Therefore, our data provided the first evidence for the potential effect of maternal exposure to the high-fat diet on the Notch signaling pathway in offspring's NSCs, indicating this altered signaling response might contribute to a profound change in offspring's brains as a result of maternal high-fat diet prior to and during gestation.
Department of Nutrition and Food Science, School of Public Health, Tianjin Medical University, Tianjin 300070, P.R. China.
Min
M
Yu
Department of Nutrition and Food Science, School of Public Health, Tianjin Medical University, Tianjin 300070, P.R. China.
Mingyue
M
Jiang
Tianjin Medical University Eye Hospital & Eye Institute, Tianjin, 300384, P.R. China.
Chunbo
C
Yang
Tianjin Medical University Eye Hospital & Eye Institute, Tianjin, 300384, P.R. China.
Yixiang
Y
Wu
Department of Toxicology, School of Public Health, Tianjin Medical University, Tianjin 300070, P.R. China.
Yongzhe
Y
Liu
Department of Clinical hematological Examination, School of Medical Laboratory, Tianjin Medical University, Tianjin 300203, P.R. China.
Yujie
Y
Cui
Department of Nutrition and Food Science, School of Public Health, Tianjin Medical University, Tianjin 300070, P.R. China. Electronic address: huangguowei@tmu.edu.cn.
Guowei
G
Huang
D000818:Animals; D000831:Animals, Newborn; D059305:Diet, High-Fat; D005260:Female; D051379:Mice; D058953:Neural Stem Cells / Q000378:metabolism*; D011247:Pregnancy; D060888:Real-Time Polymerase Chain Reaction; D051880:Receptors, Notch / Q000378:metabolism*; D015398:Signal Transduction
D051880:Receptors, Notch
HFD; Maternal high-fat diet; NSCs; Neural stem cell; Notch signaling pathway; TC; TG; high-fat diet; neural stem cells; total cholesterol; triglyceride