Canine parvovirus VP2 protein expressed in silkworm pupae self-assembles into virus-like particles with high immunogenicity.
The VP2 structural protein of parvovirus can produce virus-like particles (VLPs) by a self-assembly process in vitro, making VLPs attractive vaccine candidates. In this study, the VP2 protein of canine parvovirus (CPV) was expressed using a baculovirus expression system and assembled into parvovirus-like particles in insect cells and pupae. Electron micrographs of VLPs showed that they were very similar in size and morphology when compared to the wild-type parvovirus. The immunogenicity of the VLPs was investigated in mice and dogs. Mice immunized intramuscularly with purified VLPs, in the absence of an adjuvant, elicited CD4(+) and CD8(+) T cell responses and were able to elicit a neutralizing antibody response against CPV, while the oral administration of raw homogenates containing VLPs to the dogs resulted in a systemic immune response and long-lasting immunity. These results demonstrate that the CPV-VLPs stimulate both cellular and humoral immune responses, and so CPV-VLPs may be a promising candidate vaccine for the prevention of CPV-associated disease.
Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, Jilin Province, China.
Hao
H
Feng
Agricultural Division, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin Province, China ; College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin Province, China.
Gui-qiu
GQ
Hu
Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, Jilin Province, China.
Hua-lei
HL
Wang
Agricultural Division, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin Province, China.
Meng
M
Liang
Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, Jilin Province, China.
Hongru
H
Liang
Agricultural Division, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin Province, China.
He
H
Guo
Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Bejing, China.
Pingsen
P
Zhao
Agricultural Division, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin Province, China.
Yu-jiao
YJ
Yang
Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, Jilin Province, China.
Xue-xing
XX
Zheng
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
Zhi-fang
ZF
Zhang
Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, Jilin Province, China.
Yong-kun
YK
Zhao
Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, Jilin Province, China.
Yu-wei
YW
Gao
Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, Jilin Province, China.
Song-tao
ST
Yang
Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, Jilin Province, China.
Xian-zhu
XZ
Xia
D000818:Animals; D000906:Antibodies / Q000276:immunology; D015153:Blotting, Western; D012831:Bombyx / Q000378:metabolism*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology; D049109:Cell Proliferation; D004283:Dog Diseases / Q000276:immunology / Q000517:prevention & control; D004285:Dogs; D004912:Erythrocytes / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006384:Hemagglutination; D006385:Hemagglutination Inhibition Tests; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D017993:Parvovirus, Canine / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011679:Pupa / Q000378:metabolism; D011995:Recombination, Genetic / Q000235:genetics; D034421:Sus scrofa; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014765:Viral Vaccines / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D014771:Virion / Q000276:immunology* / Q000378:metabolism* / Q000648:ultrastructure; D019065:Virus Assembly
D000906:Antibodies; D014764:Viral Proteins; D014765:Viral Vaccines