Identification of Scopulariopsis species by partial 28S rRNA gene sequence analysis.
The genus Scopulariopsis contains over 30 species of mitosporic moulds, which although usually saprophytic may also act as opportunistic pathogens in humans. They have mainly been associated with onychomycosis, and only sporadically reported as a cause of deep tissue infections or systemic disease. Identification of Scopulariopsis species still largely relies on phenotype-based methods. There is a need for a molecular diagnostic approach, that would allow to reliably discriminate between different Scopulariopsis species. The aim of this study was to apply sequence analysis of partial 28S rRNA gene for species identification of Scopulariopsis clinical isolates. Although the method employed did reveal some genetic polymorphism among Scopulariopsis isolates tested, it was not enough for species delineation. For this to be achieved, other genetic loci, within and beyond the rDNA operon, need to be investigated.
Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw t.jagielski@biol.uw.edu.pl
Tomasz
T
Jagielski
Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw
Kinga
K
Kosim
Department of Mycology, Chair of Microbiology, Collegium Medicum, Jagiellonian University, Cracow.
Magdalena
M
Skóra
Department of Mycology, Chair of Microbiology, Collegium Medicum, Jagiellonian University, Cracow.
Anna Barbara
AB
Macura
Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw
Jacek
J
Bielecki
D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques / Q000379:methods*; D009181:Mycoses / Q000382:microbiology*; D010802:Phylogeny; D012339:RNA, Ribosomal, 28S / Q000235:genetics*; D060645:Scopulariopsis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012339:RNA, Ribosomal, 28S