Chronic exposure to Rhodobacter sphaeroides extract Lycogen™ prevents UVA-induced malondialdehyde accumulation and procollagen I down-regulation in human dermal fibroblasts.
UVA contributes to the pathogenesis of skin aging by downregulation of procollagen I content and induction of matrix metalloproteinase (MMP)-associated responses. Application of antioxidants such as lycopene has been demonstrated as a convenient way to achieve protection against skin aging. Lycogen™, derived from the extracts of Rhodobacter sphaeroides, exerts several biological effects similar to that of lycopene whereas most of its anti-aging efficacy remains uncertain. In this study, we attempted to examine whether Lycogen™ could suppress malondialdehyde (MDA) accumulation and restore downregulated procollagen I expression induced by UVA exposure. In human dermal fibroblasts Hs68 cells, UVA repressed cell viability and decreased procollagen I protein content accompanied with the induction of MMP-1 and MDA accumulation. Remarkably, incubation with 50 µM Lycogen™ for 24 h ameliorated UVA-induced cell death and restored UVA-induced downregulation of procollagen in a dose-related manner. Lycogen™ treatment also prevented the UVA-induced MMP-1 upregulation and intracellular MDA generation in Hs68 cells. Activation of NFκB levels, one of the downstream events induced by UVA irradiation and MMP-1 induction, were also prevented by Lycogen™ administration. Taken together, our findings demonstrate that Lycogen™ may be an alternative agent that prevents UVA-induced skin aging and could be used in cosmetic and pharmaceutical applications.
Department of Health and Nutrition, Chia-Nan University of Pharmacy and Science, Tainan 71710, Taiwan. connie@mail.chna.edu.tw.
Tsai-Hsiu
TH
Yang
Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei 11217, Taiwan. d49405004@gmail.com.
Ying-Hsiu
YH
Lai
Department of Health and Nutrition, Chia-Nan University of Pharmacy and Science, Tainan 71710, Taiwan. tplin007@mail.chna.edu.tw.
Tsuey-Pin
TP
Lin
Asia-Pacific Biotech Developing, Inc., Kaohsiung 80681, Taiwan. wensheng5394@gmail.com.
Wen-Sheng
WS
Liu
Department of Health and Nutrition, Chia-Nan University of Pharmacy and Science, Tainan 71710, Taiwan. lichunkuan@hotmail.com.
Li-Chun
LC
Kuan
Department and Institute of Cosmetic Science, Chia-Nan University of Pharmacy and Science, Tainan 71710, Taiwan. ccliu@mail.chna.edu.tw.
Chia-Chyuan
CC
Liu
D001688:Biological Products / Q000494:pharmacology* / Q000633:toxicity; D016923:Cell Death / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D024042:Collagen Type I / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism* / Q000528:radiation effects; D006801:Humans; D008315:Malondialdehyde / Q000378:metabolism*; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D011347:Procollagen / Q000378:metabolism*; D012242:Rhodobacter sphaeroides / Q000737:chemistry*; D014466:Ultraviolet Rays
D001688:Biological Products; D024042:Collagen Type I; D016328:NF-kappa B; D011347:Procollagen; D008315:Malondialdehyde; D020781:Matrix Metalloproteinase 1