Control of gold nanoparticles based on circular DNA strand displacement.
In this study, DNA strand displacement is utilized to control the aggregation of DNA/gold nanoparticles (AuNPs) based on circular DNA, in which DNA/AuNP conjugates are captured and released by adding different DNA signal strands. Using this strategy, single DNA/nanoparticle building blocks are capable of assembling into complex structures of two and three circular DNA/nanoparticles. The existence of these structures is demonstrated by gel electrophoresis and transmission electron microscopy (TEM) analysis. This advance has potential applications in controlling, transporting and detecting DNA/AuNP conjugates with subsequent manipulation of the structure and function of these assemblies.
Institute of Software, School of Electronics Engineering and Computer Science, Key Laboratory of High Confidence Software Technologies of Ministry of Education, Peking University, No. 5 Yiheyuan Road, Haidian District, Beijing 100871, People's Republic of China. Electronic address: zhangcheng369@gmail.com.
Cheng
C
Zhang
Institute of Software, School of Electronics Engineering and Computer Science, Key Laboratory of High Confidence Software Technologies of Ministry of Education, Peking University, No. 5 Yiheyuan Road, Haidian District, Beijing 100871, People's Republic of China. Electronic address: casy-20@126.com.
Jingjing
J
Ma
School of Control and Computer Engineering, North China Electric Power University, No. 2 Beinong Road, Huilongguan, Changping District, Beijing 102206, People's Republic of China. Electronic address: yangjing369@gmail.com.
Jing
J
Yang
College of Life Science, Shaanxi Normal University, 199 South Chang'an Road, Xi'an 710062, People's Republic of China. Electronic address: dongyf@snnu.edu.cn.
Yafei
Y
Dong
Institute of Software, School of Electronics Engineering and Computer Science, Key Laboratory of High Confidence Software Technologies of Ministry of Education, Peking University, No. 5 Yiheyuan Road, Haidian District, Beijing 100871, People's Republic of China. Electronic address: DNA@pku.edu.cn.
Jin
J
Xu
D004270:DNA, Circular / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D005427:Flocculation; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D009693:Nucleic Acid Hybridization; D013499:Surface Properties
D004270:DNA, Circular; D004277:DNA, Single-Stranded; D006046:Gold
Circular DNA; DNA self-assembly; DNA strand displacement; Gold nanoparticles; TEM image