Tolerance of apexes of coral Pocillopora damicornis L. to cryoprotectant solutions.
In this study, we investigated the tolerance of Pocillopora damicornis apexes to treatments with solutions containing penetrating and non-penetrating cryoprotective agents (CPAs). CPAs were employed individually or in binary, tertiary or quaternary solutions. In some experiments apexes were treated successively with two CPA solutions with increasing total concentration. P. damicornis apexes withstood exposure for up to 30 min to solutions containing 0.6-0.8 M sucrose (Suc) or trehalose (Tre). When apexes were treated with binary cryoprotectant solutions containing Suc and ethylene glycol (EG), methanol (Meth), dimethyl sulfoxide (Me(2)SO) or glycerol (Gly), the CPAs employed in combination with Suc could be ranked in the following order of decreasing tolerance: EG>Meth>Me(2)SO>Gly. P. damicornis apexes tolerated exposure to complex CPA solutions containing Suc, Me(2)SO, EG and/or Meth with a total molarity of 2.45 M. In experiments where two successive CPA solutions were employed, apexes withstood treatment with the second, more concentrated solution at 0°C for up to 10 min. These preliminary results pave the way to the development of a cryopreservation protocol for P. damicornis apexes.
Sorbonne Universités, UPMC Univ. Paris 06, UMS 2348, Centre de Ressources Biologiques Marines, Observatoire Océanologique de Banyuls sur mer, F-75005 Paris, France.
Lionel
L
Feuillassier
Sorbonne Universités, UPMC Univ. Paris 06, UMS 2348, Centre de Ressources Biologiques Marines, Observatoire Océanologique de Banyuls sur mer, F-75005 Paris, France.
Pascal
P
Romans
IRD, UMR DIADE, 911 Avenue Agropolis, BP 64501, 34394 Montpellier Cedex 5, France.
Isabelle
I
Engelmann-Sylvestre
Aquarium de Canet-en-Roussillon, 2 boulevard de la Jetée, 66140 Canet-en-Roussillon, France.
Patrick
P
Masanet
Océanopolis, Port de plaisance du Moulin Blanc, BP 91039, 29210 Brest Cedex 1, France.
Dominique
D
Barthélémy
IRD, UMR DIADE, 911 Avenue Agropolis, BP 64501, 34394 Montpellier Cedex 5, France. Electronic address: florent.engelmann@ird.fr.
Florent
F
Engelmann
D000222:Adaptation, Physiological; D000818:Animals; D037421:Anthozoa / Q000187:drug effects* / Q000502:physiology; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D019855:Ethylene Glycol / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D000432:Methanol / Q000494:pharmacology; D009994:Osmolar Concentration; D009997:Osmotic Pressure; D019946:Propylene Glycol / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology; D014199:Trehalose / Q000494:pharmacology
D003451:Cryoprotective Agents; D013395:Sucrose; D019946:Propylene Glycol; D014199:Trehalose; D019855:Ethylene Glycol; D005990:Glycerol; D000432:Methanol; D004121:Dimethyl Sulfoxide
Apex; Coral; Cryopreservation; Cryoprotectant solutions; Pocillopora damicornis