Identification of Echinococcus granulosus Strains in Isolated Hydatid Cyst Specimens from Animals by PCR-RFLP Method in West Azerbaijan - Iran.
BACKGROUND
The aim of this study was DNA extraction from protoscolecses of Echinococcus granulosus and identification of these strains in West-Azerbaijan Province, north western Iran.


METHODS
Thirty one livestock isolates from sheep and cattle were collected from abattoirs of the province. To investigate the genetic variation of the isolates, after DNA extraction by Glass beads-phenol chloroform method; PCR-RLFP analysis of rDNA-ITS1 was performed using three different restriction enzymes of Taq 1, Rsa 1 and Alu 1.


RESULTS
Amplified PCR products for all isolates were 1000bp band which is expected band in sheep strains (G1-G3 complex). The results of RFLP analysis also were the same for all isolates. PCR-RFLP patterns restriction enzymes were identical as follows, Rsa1 bands under UV showed two bands approximately 655bp and 345bp. Alu1 bands were as follows: two approximately 800bp and 200bp and Taq1 did not cut any region and bands were approximately 1000 bp in all samples.


CONCLUSIONS
Based on PCR-RFLP patterns of ITS1 fragment produced with endonucleases enzyme digestion in animal isolates, it can be concluded that a single strain of E. granulosus (sheep strain or G1-G3 complex) is dominant genotype in this province.
Dept. of Medical Parasitology and Mycology, Faculty of medicine, Urmia University of Medical Sciences, Urmia, Iran.
Haleh
H
Hanifian
Dept. of Medical Parasitology and Mycology, Faculty of medicine, Urmia University of Medical Sciences, Urmia, Iran.
Kambiz
K
Diba
Cellular and Molecular Research center, Urmia University of Medical Seiences, Urmia-Iran.
Khosrow Hazrati
KH
Tappeh
Dept. of Medical Parasitology and Mycology, Faculty of medicine, Urmia University of Medical Sciences, Urmia, Iran.
Habib
H
Mohammadzadeh
Dept. of surgery, Faculty of medicine, Urmia University of Medical Sciences, Urmia-Iran.
Rahim
R
Mahmoudlou
Hydatid cyst; ITS1; RFLP-PCR; Strain