Comparative assessment of automated nucleic acid sample extraction equipment for biothreat agents.
Magnetic beads offer superior impurity removal and nucleic acid selection over older extraction methods. The performances of nucleic acid extraction of biothreat agents in blood or buffer by easyMAG, MagNA Pure, EZ1 Advanced XL, and Nordiag Arrow were evaluated. All instruments showed excellent performance in blood; however, the easyMAG had the best precision and versatility.
Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Disease, Fort Detrick, Maryland, USA.
Warren Vincent
WV
Kalina
Christina Elizabeth
CE
Douglas
Susan Rajnik
SR
Coyne
Timothy Devin
TD
Minogue
D057205:Automation, Laboratory / Q000379:methods*; D054045:Biological Warfare Agents; D006801:Humans; D008828:Microbiological Techniques / Q000295:instrumentation / Q000379:methods*; D009696:Nucleic Acids / Q000097:blood* / Q000302:isolation & purification*
D054045:Biological Warfare Agents; D009696:Nucleic Acids