MiR-98 is involved in rat embryo implantation by targeting Bcl-xl.
In a previous study, via microRNA microarray analysis we found that miR-98 is differentially expressed in rat uteri during the peri-implantation period (unpublished data). However, the role of miR-98 in rat embryo implantation remains elusive. Here, we found that the level of miR-98 is lower on day 5 and 6 of gestation (g.d. 5-6) than that on g.d. 3-4 and g.d. 7-8 in rat. MiR-98 expression is significantly decreased by delayed implantation. Down-regulation of miR-98 promotes ESC proliferation and inhibits apoptosis. Up-regulation of miR-98 displays opposite effects. Further investigation revealed that miR-98 can bind to the 3'-untranslated region (3'-UTR) of B-cell lymphoma-extra large (Bcl-xl) to inhibit Bcl-xl translation. Collectively, down-regulation of miR-98 in rat uterus during the receptive phase is linked to the increase of cell proliferation via targeting Bcl-xl.
Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing 100081, China; Graduate School, Peking Union Medical College, Beijing, China. Electronic address: hongfeixia@126.com.
Hong-Fei
HF
Xia
Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing 100081, China.
Xiao-Hua
XH
Jin
Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing 100081, China.
Zong-Fu
ZF
Cao
Haidian Maternal & Child Health Hospital, Beijing, China.
Tian
T
Shi
Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing 100081, China; Graduate School, Peking Union Medical College, Beijing, China. Electronic address: genetic@263.net.cn.
Xu
X
Ma
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D001483:Base Sequence; D049109:Cell Proliferation / Q000187:drug effects; D010064:Embryo Implantation / Q000187:drug effects / Q000235:genetics*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014599:Uterus / Q000166:cytology / Q000187:drug effects / Q000196:embryology / Q000378:metabolism; D051020:bcl-X Protein / Q000235:genetics*
C571630:MIRN98 microRNA-98, rat; D035683:MicroRNAs; D051020:bcl-X Protein; D011374:Progesterone
Bcl-xl; Embryo implantation; Rat; Uterus; miR-98