Behaviour of cytoplasmic organelles and cytoskeleton during oocyte maturation.
Assisted reproduction technology (ART) has become an attractive option for infertility treatment and holds tremendous promise. However, at present, there is still room for improvement in its success rates. Oocyte maturation is a process by which the oocyte becomes competent for fertilization and subsequent embryo development. To better understand the mechanism underlying oocyte maturation and for the future improvement of assisted reproduction technology, this review focuses on the complex processes of cytoplasmic organelles and the dynamic alterations of the cytoskeleton that occur during oocyte maturation. Ovarian stimulation and in-vitro maturation are the major techniques used in assisted reproduction technology and their influence on the organelles of oocytes is also discussed. Since the first birth by assisted reproduction treatment was achieved in 1978, numerous techniques involved in assisted reproduction have been developed and have become attractive options for infertility treatment. However, the unsatisfactory success rate remains as a main challenge. Oocyte maturation is a process by which the oocyte becomes competent for fertilization and subsequent embryo development. Oocyte maturation includes both nuclear and cytoplasmic maturation. Nuclear maturation primarily involves chromosomal segregation, which has been well studied, whereas cytoplasmic maturation involves a series of complicated processes, and there are still many parts of this process that remain controversial. Ovarian stimulation and in-vitro maturation (IVM) are the major techniques of assisted reproduction. The effect of ovarian stimulation or IVM on the behaviour of cell organelles of the oocyte has been postulated as the reason for the reduced developmental potential of in-vitro-produced embryos. To further understanding of the mechanism of oocyte maturation and future improvement of assisted reproduction treatment, the complex events of cytoplasmic organelles and the cytoskeleton that occur during oocyte maturation and the influence of ovarian stimulation and IVM on these organelles are described in this review.
Center of Reproductive Medicine, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.
Luna
L
Mao
Center of Reproductive Medicine, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.
Hangying
H
Lou
Center of Reproductive Medicine, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China; Hangzhou Hospital of Traditional Chinese Medicine, Hangzhou 310006, China.
Yiyun
Y
Lou
Center of Reproductive Medicine, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.
Ning
N
Wang
Center of Reproductive Medicine, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China; Key Laboratory of Reproductive Genetics, Zhejiang University, Ministry of Education, Women's Reproductive Health Laboratory of Zhejiang, Hangzhou 310006, China. Electronic address: jinfan@zju.edu.cn.
Fan
F
Jin
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D003593:Cytoplasm / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D005306:Fertilization / Q000502:physiology; D006056:Golgi Apparatus / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D006801:Humans; D051379:Mice; D008928:Mitochondria / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D008954:Models, Biological; D009865:Oocytes / Q000254:growth & development* / Q000648:ultrastructure; D015388:Organelles / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure
D000255:Adenosine Triphosphate; D002118:Calcium
assisted reproduction technology; cytoplasmic organelles; cytoskeleton; fertilization; oocyte maturation; oocyte quality