Accumulation and effects of the UV-filter octocrylene in adult and embryonic zebrafish (Danio rerio).
Wide application of the UV-filter octocrylene (OC) in cosmetics leads to contamination of the aquatic environment, but effects of OC remain unclear. Here we determine bioaccumulation and molecular effects of OC. Adult male zebrafish were exposed to 22, 209 and 383 μg/L and embryos to 69, 293 and 925 μg/L OC. OC accumulated in fish up to 17 μg/g. Calculated BCF varied between 41 and 136. Microarray analysis in brain and liver following exposure to 383 μg/L OC revealed alteration of 628 and 136 transcripts, respectively. Most prominent GO processes included developmental processes, organ development, hematopoiesis, formation of blood vessels, blood circulation, fat cell differentiation and metabolism. Validation by RT-qPCR in brain and liver of adult fish and embryos included a series of genes. Blood levels of 11-ketotestosterone were not altered. The transcriptomics data suggest that OC mainly affects transcription of genes related to developmental processes in the brain and liver as well as metabolic processes in the liver.
University of Applied Sciences Northwestern Switzerland, School of Life Sciences, Gründenstrasse 40, 4132 Muttenz, Switzerland; University of Basel, Division of Molecular and Systems Toxicology, Department of Pharmaceutical Sciences, Klingelbergstrasse 50, 4056 Basel, Switzerland.
Nancy
N
Blüthgen
University of Applied Sciences Northwestern Switzerland, School of Life Sciences, Gründenstrasse 40, 4132 Muttenz, Switzerland.
Nicole
N
Meili
University of Applied Sciences Northwestern Switzerland, School of Life Sciences, Gründenstrasse 40, 4132 Muttenz, Switzerland.
Geraldine
G
Chew
University of Basel, Division of Molecular and Systems Toxicology, Department of Pharmaceutical Sciences, Klingelbergstrasse 50, 4056 Basel, Switzerland.
Alex
A
Odermatt
University of Applied Sciences Northwestern Switzerland, School of Life Sciences, Gründenstrasse 40, 4132 Muttenz, Switzerland; Swiss Federal Institute of Technology (ETH Zürich), Institute of Biogeochemistry and Pollution Dynamics, Department of Environmental System Sciences, 8092 Zürich, Switzerland. Electronic address: karl.fent@fhnw.ch.
Karl
K
Fent
D000179:Acrylates / Q000378:metabolism / Q000633:toxicity*; D000818:Animals; D004625:Embryo, Nonmammalian / Q000187:drug effects*; D008297:Male; D013473:Sunscreening Agents / Q000378:metabolism / Q000633:toxicity*; D013739:Testosterone / Q000031:analogs & derivatives / Q000097:blood; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*; D015027:Zebrafish / Q000378:metabolism
D000179:Acrylates; D013473:Sunscreening Agents; D014874:Water Pollutants, Chemical; D013739:Testosterone; C088673:octocrylene; C003600:11-ketotestosterone
Bioaccumulation; Microarray; Octocrylene; Personal care products; Transcriptomics; UV-filter