High-efficiency solid phase peptide synthesis (HE-SPPS).
A series of improvements to the standard solid phase peptide synthesis (SPPS) process allowing for significant gains in product purity along with only a 4 min standard cycle time and a 90% reduction in total waste produced is reported. For example, syntheses of the well-known (65-74)acyl carrier protein (ACP) and (1-42)β-amyloid peptides were accomplished with 93 and 72% purity (UPLC-MS) in only 44 and 229 min, respectively.
CEM Corporation , 3100 Smith Farm Road, Matthews, North Carolina 28104, United States.
Jonathan M
JM
Collins
Keith A
KA
Porter
Sandeep K
SK
Singh
Grace S
GS
Vanier
D000213:Acyl Carrier Protein / Q000138:chemical synthesis* / Q000737:chemistry; D016229:Amyloid beta-Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000737:chemistry; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000737:chemistry; D060327:Solid-Phase Synthesis Techniques
D000213:Acyl Carrier Protein; D016229:Amyloid beta-Peptides; D009842:Oligopeptides; D010446:Peptide Fragments