Biodegradable magnetic calcium phosphate nanoformulation for cancer therapy.
We fabricated a magnetic calcium phosphate nanoformulation by the biomineralization of calcium phosphate on the surface of magnetic nanoparticles with abundant amino groups, and thus the inorganic layer of calcium phosphate can improve the biocompatibility and simultaneously the magnetic iron oxide can maintain the magnetic targeting function. Two types of anticancer drug models, doxorubicin hydrochloride and DNA, were entrapped in these nanocarriers, respectively. This delivery system displayed high pH sensitivity in drug-controlled release profile as the dissolution of CaP under acid pH condition. Magnetofection was performed to investigate the intracellular uptake and the anti-proliferative effect of tumor cells in the presence of an external magnet. The transfection of the DNA-loaded magnetic system in A549 and HepG2 tumor cells demonstrated that the magnetic nanoformulation could enhance the transfection efficiency to 30% with an applied external magnetic field.
Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, PR China.
Zhaomin
Z
Tang
Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, PR China; School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, PR China.
Yangbo
Y
Zhou
Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, PR China; School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, PR China.
Huili
H
Sun
Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, PR China.
Dan
D
Li
Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, PR China; School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, PR China. Electronic address: shaobingzhou@hotmail.com.
Shaobing
S
Zhou
D000970:Antineoplastic Agents / Q000008:administration & dosage*; D001672:Biocompatible Materials / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D004317:Doxorubicin / Q000008:administration & dosage*; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D004355:Drug Stability; D005434:Flow Cytometry; D018014:Gene Transfer Techniques; D056945:Hep G2 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D013054:Spectrophotometry, Atomic; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D014162:Transfection
D000970:Antineoplastic Agents; D001672:Biocompatible Materials; D002130:Calcium Phosphates; D004337:Drug Carriers; D058185:Magnetite Nanoparticles; D004317:Doxorubicin; D004247:DNA; C020243:calcium phosphate
Calcium phosphate; Drug delivery; Gene transfection; Magnetic targeting; Nanoparticle; pH sensitivity