Resveratrol ameliorates diabetic vascular inflammation and macrophage infiltration in db/db mice by inhibiting the NF-κB pathway.
In this study, resveratrol (RSV) - a potent sirtuin 1 activator - was found to have beneficial effects on glucolipid metabolism and improve inflammatory mediators and markers of oxidative stress. Diabetic (db/db) mice and non-diabetic C57BL/6J mice were used in the study. The db/db mice were treated with or without 0.3% RSV mixed with chow for 8 weeks. Dietary RSV significantly lowered blood glucose, plasma lipid and free fatty acid levels in db/db mice. RSV markedly inhibited the expression of intercellular adhesion molecule-1 (ICAM-1), endothelial vascular cell adhesion molecule-1 (VCAM-1) and monocyte chemoattractant protein-1 (MCP-1) in the aorta and the blood plasma of db/db mice (p < 0.05). Levels of mac-3-positive macrophages (measure of the infiltration of activated macrophages) were lower in RSV-treated diabetic mice than in their untreated counterparts (p < 0.05). RSV treatment reduced the activity of the transcriptional regulator nuclear factor kappa B (NF-κB) in aortic tissues (p < 0.05). Thus, RSV treatment reduced ICAM-1, VCAM-1 and MCP-1 expression in the aorta and ICAM-1, VCAM-1 and MCP-1 levels in the plasma of diabetic mice. Since dietary supplementation with RSV also reduced NF-κB activities in the aorta, the therapeutic effects of RSV might be associated with the downregulation of NF-κB.
Department of Cardiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, PR China.
Rong
R
Guo
Baoxin
B
Liu
Ke
K
Wang
Shunping
S
Zhou
Weiming
W
Li
Yawei
Y
Xu
D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D018932:Chemokine CCL2 / Q000187:drug effects* / Q000378:metabolism; D048909:Diabetes Complications / Q000378:metabolism*; D003920:Diabetes Mellitus; D007249:Inflammation / Q000188:drug therapy; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism; D000077185:Resveratrol; D015398:Signal Transduction / Q000187:drug effects*; D013267:Stilbenes / Q000494:pharmacology*; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
D000943:Antigens, Differentiation; D001786:Blood Glucose; D018932:Chemokine CCL2; D016328:NF-kappa B; D013267:Stilbenes; D019010:Vascular Cell Adhesion Molecule-1; C036763:monocyte-macrophage differentiation antigen; D018799:Intercellular Adhesion Molecule-1; D000077185:Resveratrol
Diabetes; NF-κB; SIRT1; resveratrol; vascular inflammation