Developmental competence of frozen-thawed yak (Bos grunniens) oocytes followed by in vitro maturation and fertilization.
In the present study, we examined the ability of immature germinal vesicle (GV) and subjected to in vitro matured (MII) yak oocytes to survive after cryopreservation as well as their subsequent development following in vitro maturation and fertilization. Both GV and MII oocytes were cryopreserved by using two different vitrification solutions (VS); VS-I contained 10% ethylene glycol (EG) and 10% dimethylsulfoxide (DMSO) in TCM-199 + 20% (v/v) fetal calf serum (FCS) whereas VS-II contained 40% EG + 18% Ficoll + 0.5 M sucrose in TCM-199 + 20% FCS. The percentage of oocytes found to be morphologically normal was greater (P < 0.01) in VS-I group than in VS-II group. Rates of cleavage (30.6-42.2%) and blastocyst formation (2.9-8.9%) did not differ among groups, but were lower than in unfrozen control (55.7% and 25.4%, P < 0.01). These results show that a combination of EG and DMSO or EG, Ficoll and sucrose can be used to cryopreserve yak oocytes in French straws.
The Key Laboratory of Animal Genetics and Breeding, Ministry of Education and State Ethnic Affairs Commission, Southwest University for Nationalities, Chengdu, Sichuan 610041, PR China.
Hui-Ran
HR
Niu
The Key Laboratory of Animal Genetics and Breeding, Ministry of Education and State Ethnic Affairs Commission, Southwest University for Nationalities, Chengdu, Sichuan 610041, PR China. Electronic address: zixd2000@yahoo.com.
Xiang-Dong
XD
Zi
The Key Laboratory of Animal Genetics and Breeding, Ministry of Education and State Ethnic Affairs Commission, Southwest University for Nationalities, Chengdu, Sichuan 610041, PR China.
Xiao
X
Xiao
The Key Laboratory of Animal Genetics and Breeding, Ministry of Education and State Ethnic Affairs Commission, Southwest University for Nationalities, Chengdu, Sichuan 610041, PR China.
Xian-Rong
XR
Xiong
The Key Laboratory of Animal Genetics and Breeding, Ministry of Education and State Ethnic Affairs Commission, Southwest University for Nationalities, Chengdu, Sichuan 610041, PR China.
Jin-Cheng
JC
Zhong
The Key Laboratory of Animal Genetics and Breeding, Ministry of Education and State Ethnic Affairs Commission, Southwest University for Nationalities, Chengdu, Sichuan 610041, PR China.
Jian
J
Li
The Key Laboratory of Animal Genetics and Breeding, Ministry of Education and State Ethnic Affairs Commission, Southwest University for Nationalities, Chengdu, Sichuan 610041, PR China.
Li
L
Wang
The Key Laboratory of Animal Genetics and Breeding, Ministry of Education and State Ethnic Affairs Commission, Southwest University for Nationalities, Chengdu, Sichuan 610041, PR China.
Yong
Y
Wang
D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D003470:Culture Media; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004622:Embryo, Mammalian; D047108:Embryonic Development / Q000187:drug effects; D019855:Ethylene Glycol / Q000494:pharmacology; D005260:Female; D005306:Fertilization / Q000187:drug effects* / Q000502:physiology; D005307:Fertilization in Vitro; D005362:Ficoll / Q000494:pharmacology; D008297:Male; D009865:Oocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D009994:Osmolar Concentration; D013395:Sucrose / Q000494:pharmacology; D058989:Vitrification
D003451:Cryoprotective Agents; D003470:Culture Media; D005362:Ficoll; D013395:Sucrose; D019855:Ethylene Glycol; D004121:Dimethyl Sulfoxide
Cryopreservation; In vitro development; In vitro fertilization; In vitro maturation; Yak oocytes