DNA directed immobilization glycocluster array: applications and perspectives.
The present review concerns the recent advances in DNA directed immobilization (DDI) based glycocluster array. The impact of glycan immobilization on subsequent interactions with protein is discussed and the consequent pros and cons of DDI-based glycocluster array are reviewed. Finally, application in the discovery of anti-pathogen molecules is illustrated by screening for galactose or fucose glycoclusters targeting two Pseudomonas aeruginosa virulence factors (PA-IL and PA-IIL).
Université de Lyon, Institut des Nanotechnologies de Lyon UMR CNRS 5270, Ecole Centrale de Lyon, 36 Avenue Guy de Collongue, 69134 Ecully Cedex, France. Electronic address: yann.chevolot@ec-lyon.fr.
Yann
Y
Chevolot
Université de Lyon, Institut des Nanotechnologies de Lyon UMR CNRS 5270, Ecole Centrale de Lyon, 36 Avenue Guy de Collongue, 69134 Ecully Cedex, France.
Emmanuelle
E
Laurenceau
Université de Lyon, Institut des Nanotechnologies de Lyon UMR CNRS 5270, Ecole Centrale de Lyon, 36 Avenue Guy de Collongue, 69134 Ecully Cedex, France.
Magali
M
Phaner-Goutorbe
Université de Lyon, Institut des Nanotechnologies de Lyon UMR CNRS 5270, Ecole Centrale de Lyon, 36 Avenue Guy de Collongue, 69134 Ecully Cedex, France.
Virginie
V
Monnier
Université de Lyon, Institut des Nanotechnologies de Lyon UMR CNRS 5270, Ecole Centrale de Lyon, 36 Avenue Guy de Collongue, 69134 Ecully Cedex, France.
Eliane
E
Souteyrand
Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS - Université Montpellier 1 - Université Montpellier 2, Place Eugène Bataillon, CC1704, 34095 Montpellier Cedex 5, France.
Albert
A
Meyer
Université de Lyon, Institut des Nanotechnologies de Lyon UMR CNRS 5270, Ecole Centrale de Lyon, 36 Avenue Guy de Collongue, 69134 Ecully Cedex, France.
Thomas
T
Géhin
Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS - Université Montpellier 1 - Université Montpellier 2, Place Eugène Bataillon, CC1704, 34095 Montpellier Cedex 5, France.
Jean-Jacques
JJ
Vasseur
Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS - Université Montpellier 1 - Université Montpellier 2, Place Eugène Bataillon, CC1704, 34095 Montpellier Cedex 5, France. Electronic address: morvan@univ-montp2.fr.
François
F
Morvan
D004247:DNA / Q000378:metabolism*; D005643:Fucose / Q000032:analysis* / Q000378:metabolism; D005690:Galactose / Q000032:analysis* / Q000378:metabolism; D054794:Glycomics / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000378:metabolism; D037521:Virulence Factors / Q000032:analysis / Q000378:metabolism
D037521:Virulence Factors; D005643:Fucose; D004247:DNA; D005690:Galactose