Cell patterning by laser-assisted bioprinting.
The aim of tissue engineering is to produce functional three-dimensional (3D) tissue substitutes. Regarding native organ and tissue complexity, cell density and cell spatial 3D organization, which influence cell behavior and fate, are key parameters in tissue engineering. Laser-Assisted Bioprinting (LAB) allows one to print cells and liquid materials with a cell- or picoliter-level resolution. Thus, LAB seems to be an emerging and promising technology to fabricate tissue-like structures that have the physiological functionality of their native counterparts. This technology has additional advantages such as automation, reproducibility, and high throughput. It makes LAB compatible with the (industrial) fabrication of 3D constructs of physiologically relevant sizes. Here we present exhaustively the numerous steps that allow printing of viable cells with a well-preserved micrometer pattern. To facilitate the understanding of the whole cell patterning experiment using LAB, it is discussed in two parts: (1) preprocessing: laser set-up, bio-ink cartridge and bio-paper preparation, and pattern design; and (2) processing: bio-ink printing on the bio-paper.
INSERM U1026, Bordeaux, France; University of Bordeaux Segalen, Bordeaux, France.
Raphaël
R
Devillard
INSERM U1026, Bordeaux, France; University of Bordeaux Segalen, Bordeaux, France.
Emeline
E
Pagès
INSERM U1026, Bordeaux, France; University of Bordeaux Segalen, Bordeaux, France.
Manuela Medina
MM
Correa
INSERM U1026, Bordeaux, France; University of Bordeaux Segalen, Bordeaux, France.
Virginie
V
Kériquel
INSERM U1026, Bordeaux, France; University of Bordeaux Segalen, Bordeaux, France.
Murielle
M
Rémy
INSERM U1026, Bordeaux, France; University of Bordeaux Segalen, Bordeaux, France.
Jérôme
J
Kalisky
INSERM U1026, Bordeaux, France; University of Bordeaux Segalen, Bordeaux, France.
Muhammad
M
Ali
INSERM U1026, Bordeaux, France; University of Bordeaux Segalen, Bordeaux, France.
Bertrand
B
Guillotin
INSERM U1026, Bordeaux, France; University of Bordeaux Segalen, Bordeaux, France.
Fabien
F
Guillemot
D062028:Bioprinting / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007834:Lasers; D023822:Tissue Engineering / Q000379:methods*
Cell patterning; Laser-assisted bioprinting (LAB); Rapid prototyping; Tissue engineering