Near-infrared-light-mediated imaging of latent fingerprints based on molecular recognition.
Photoluminescence is one of the most sensitive techniques for fingerprint detection, but it also suffers from background fluorescence and selectivity at the expense of generality. The method described herein integrates the advantages of near-infrared-light-mediated imaging and molecular recognition. In principle, upconversion nanoparticles (UCNPs) functionalized with a lysozyme-binding aptamer were used to detect fingerprints through recognizing lysozyme in the fingerprint ridges. UCNPs possess the ability to suppress background fluorescence and make it possible for fingerprint imaging on problematic surfaces. Lysozyme, a universal compound in fingerprints, was chosen as the target, thus simultaneously meeting the selectivity and generality criteria in photoluminescence approaches. Fingerprints on different surfaces and from different people were detected successfully. This strategy was used to detect fingerprints with cocaine powder by using UCNPs functionalized with a cocaine-binding aptamer.
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 (China).
Jie
J
Wang
Ting
T
Wei
Xinyang
X
Li
Binhao
B
Zhang
Jiaxi
J
Wang
Chi
C
Huang
Quan
Q
Yuan
D052157:Aptamers, Nucleotide / Q000737:chemistry; D003042:Cocaine / Q000032:analysis*; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism; D007259:Infrared Rays; D008163:Luminescent Measurements; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*
D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D009113:Muramidase; D003042:Cocaine
aptamers; fluorescence; imaging agents; molecular recognition; nanoparticles