Changes in electrical response function and myosin heavy chain isoforms following denervation and reinnervation of bilateral posterior cricoarytenoid muscles in dogs.
CONCLUSIONS
Both electrical response function and mRNA expression of myosin heavy chain (MyHC) types 2X, 1, and Neonatal of bilateral posterior cricoarytenoid (PCA) muscle changed after denervation or reinnervation in canines.


OBJECTIVE
There is a need to investigate the electrical response function MyHC alteration of denervation or reinnervation in the bilateral PCA muscle of large animals.


METHODS
MyHC isoforms expression profile and PCA muscle function outcome were detected by real-time reverse transcribed-polymerase chain reaction and muscle response to functional electrical stimulation, 9 weeks after denervation and reinnervation with ansa-recurrent laryngeal nerve anastomosis in dogs.


RESULTS
Denervation produced up-regulation of MyHC-1 and MyHC-Neonatal messenger ribonucleic acid (mRNA) expression. Reinnervation caused a decrease of MyHC-2X mRNA expression. The electrical voltage threshold of vocal fold movement and maximum abduction of denervation were greater than that of the reinnervated or control group. The denervated vocal abduction maximum of response to electrical stimulation was less than that in reinnervation or control groups.
Department of Otolaryngology, Guangdong General Hospital & Guangdong Academy of Medical Sciences , Guangzhou city and.
Jingyuan
J
Li
Shaofeng
S
Liu
Qiuhui
Q
Cheng
Mingrong
M
Nie
Siyi
S
Zhang
Xiaoli
X
Sheng
Shaohua
S
Chen
Pingjiang
P
Ge
D000714:Anastomosis, Surgical / Q000379:methods*; D000818:Animals; D004285:Dogs; D004558:Electric Stimulation; D005786:Gene Expression Regulation / Q000502:physiology; D007821:Laryngeal Muscles / Q000294:innervation* / Q000378:metabolism; D009121:Muscle Denervation; D018995:Myosin Heavy Chains / Q000235:genetics*; D016067:Nerve Transfer / Q000379:methods*; D020033:Protein Isoforms / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D012009:Recurrent Laryngeal Nerve / Q000601:surgery*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014827:Vocal Cords / Q000294:innervation
D020033:Protein Isoforms; D012333:RNA, Messenger; D018995:Myosin Heavy Chains