Cationic lipophosphoramidates with two different lipid chains: synthesis and evaluation as gene carriers.
Cationic lipids constitute a family of synthetic vectors commonly used for nucleic acids delivery. We herein report the results of a systematic study that aimed to compare the transfection efficacies of cationic lipophosphoramidates possessing either two identical lipid chains (termed symmetric cationic lipids) or two different lipid chains (non-symmetric cationic lipids). In addition, we also compared the transfection results of such a 'molecular approach' (the two different lipid chains being included in the same molecule) with those of a 'supramolecular approach' in which two types of symmetrical cationic lipids were mixed in one liposomal formulation. Thus, the present work allowed us first to optimize the methods used to synthesize non-symmetric cationic lipophosphoramidates. In addition, we could also identify two non-symmetric cationic lipids exhibiting high transfection efficiencies with a series of mammalian cell lines, both vectors being characterized by a single phytanyl chain and either an oleyl or a lauryl lipid chain.
UMR CNRS 6521; SFR ScInBioS, Université de Bretagne Occidentale, Université Européenne de Bretagne, Faculté des Sciences, 6 avenue Victor Le Gorgeu, 29238 Brest, France. pjaffres@univ-brest.fr.
Stéphanie S
SS
Le Corre
Mathieu
M
Berchel
Nawal
N
Belmadi
Caroline
C
Denis
Jean-Pierre
JP
Haelters
Tony
T
Le Gall
Pierre
P
Lehn
Tristan
T
Montier
Paul-Alain
PA
Jaffrès
D000577:Amides / Q000737:chemistry*; D002412:Cations / Q000737:chemistry; D002460:Cell Line; D018014:Gene Transfer Techniques; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D015394:Molecular Structure; D010756:Phosphoric Acids / Q000737:chemistry*
D000577:Amides; D002412:Cations; D008055:Lipids; D010756:Phosphoric Acids; C011067:phosphoramidic acid