Cytokine expression at the anchor site in experimental Taenia solium infection in hamsters.
The establishment of Taenia solium adult parasite in the human intestine causes taeniosis. Importantly, the immunological mechanisms occurring at the interface between the parasite and its host are not fully known. The development of experimental animal models has facilitated the understanding of the host-parasite relationship. In this study we standardized a quantitative RT-PCR method for analyzing hamster messenger RNA for interferon-gamma (IFN-γ) and interleukins (IL): IL-4 IL-10, IL-12 and IL-13. This method was then used to evaluate the local cytokine response elicited against the adult parasite at the attachment site in the intestine of infected hamsters. The results showed an intense IFN-γ response, as well as an up-regulation of IL-4 as early as three days post-infection, permanence of IL-10 until the end of the experiment and down regulation of IL-12. These data are in agreement with a bias toward a Th-2 response as the infection progresses.
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, México, DF, Mexico.
Mayra
M
Cruz-Rivera
AV BioSciences, México, DF, Mexico.
Gilberto
G
Vaughan
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, México, DF, Mexico; Facultad de Ciencias de la Salud, Universidad Anáhuac México Norte, Estado de México, Mexico.
Fela
F
Mendlovic
Facultad de Ciencias Químicas, Universidad la Salle, México, DF, Mexico.
Arely
A
Vergara-Castañeda
Departamento de Ecología de Agentes Patógenos, Hospital General Dr. Manuel Gea González, Secretaria de Salud, México, DF, México.
Mirza
M
Romero-Valdovinos
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, México, DF, Mexico.
Sonia
S
Leon-Cabrera
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, México, DF, Mexico.
Monica
M
Alonso
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, México, DF, Mexico.
Guillermina
G
Avila
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, México, DF, Mexico. Electronic address: flisser@unam.mx.
Ana
A
Flisser
D000818:Animals; D006224:Cricetinae; D016207:Cytokines / Q000235:genetics*; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000276:immunology*; D006790:Host-Parasite Interactions; D041201:Taenia solium / Q000276:immunology; D013622:Taeniasis / Q000276:immunology*
D016207:Cytokines
Cytokines; Hamster model; PCR; Taenia solium; Taeniosis