Identification and expression profile analysis of putative odorant-binding proteins in Sitodiplosis mosellana (Gehin) (Diptera: Cecidomyiidae).
Odorant binding proteins (OBPs) contribute to the remarkable sensitivity of the insect's olfactory system and play important roles in the olfactory recognition. The orange blossom midge, Sitodiplosis mosellana is a cereal specialist, and utilizes pheromone and host odorant as a cue for its mating and oviposition. However, OBP genes have not been largely identified in S. mosellana. Based on the sequenced transcriptome database, twenty-six OBP genes were identified in S. mosellana for the first time. Phylogenetic analysis revealed that S. mosellana OBP genes are more closely related to Mayetiola destructor OBP genes than to Aedes aegypti OBP genes. Most OBP genes seemed to be antenna-specific, but differentially expressed in male and female antennae. Three OBP genes (OBP9, OBP19 and OBP23) are leg-specific. And also, most OBP genes have higher expression levels in adults. Only one OBP gene (OBP10) has higher expression levels in larval stages. These findings serve as an important basis for understanding the molecular mechanisms of chemosensory perception.
Institute of Plant Protection, Henan Academy of Agricultural Sciences, Key Laboratory of Crop Pest Control of Henan Province, Key Laboratory of Crop Integrated Pest Management of the Southern of North China, Ministry of Agriculture of the People's Republic of China, Zhengzhou, PR China.
Zhong-Jun
ZJ
Gong
Institute of Plant Protection, Henan Academy of Agricultural Sciences, Key Laboratory of Crop Pest Control of Henan Province, Key Laboratory of Crop Integrated Pest Management of the Southern of North China, Ministry of Agriculture of the People's Republic of China, Zhengzhou, PR China.
Jin
J
Miao
Institute of Plant Protection, Henan Academy of Agricultural Sciences, Key Laboratory of Crop Pest Control of Henan Province, Key Laboratory of Crop Integrated Pest Management of the Southern of North China, Ministry of Agriculture of the People's Republic of China, Zhengzhou, PR China.
Yun
Y
Duan
Institute of Plant Protection, Henan Academy of Agricultural Sciences, Key Laboratory of Crop Pest Control of Henan Province, Key Laboratory of Crop Integrated Pest Management of the Southern of North China, Ministry of Agriculture of the People's Republic of China, Zhengzhou, PR China.
Yue-Li
YL
Jiang
Institute of Plant Protection, Henan Academy of Agricultural Sciences, Key Laboratory of Crop Pest Control of Henan Province, Key Laboratory of Crop Integrated Pest Management of the Southern of North China, Ministry of Agriculture of the People's Republic of China, Zhengzhou, PR China.
Tong
T
Li
Institute of Plant Protection, Henan Academy of Agricultural Sciences, Key Laboratory of Crop Pest Control of Henan Province, Key Laboratory of Crop Integrated Pest Management of the Southern of North China, Ministry of Agriculture of the People's Republic of China, Zhengzhou, PR China. Electronic address: yuqingwu36@hotmail.com.
Yu-Qing
YQ
Wu
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004175:Diptera / Q000235:genetics* / Q000254:growth & development; D005260:Female; D018507:Gene Expression Regulation, Developmental; D019476:Insect Proteins / Q000145:classification / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D020033:Protein Isoforms / Q000235:genetics; D018035:Receptors, Odorant / Q000145:classification / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012737:Sex Factors; D059467:Transcriptome
D019476:Insect Proteins; D020033:Protein Isoforms; D018035:Receptors, Odorant; C045648:odorant-binding protein
Expression profile; Odorant binding protein; Real-time PCR; Sitodiplosis mosellana; Transcriptome