Translating human genetics into mouse: the impact of ultra-rapid in vivo genome editing.
Gene-targeted mutant animals, such as knockout or knockin mice, have dramatically improved our understanding of the functions of genes in vivo and the genetic diversity that characterizes health and disease. However, the generation of targeted mice relies on gene targeting in embryonic stem (ES) cells, which is a time-consuming, laborious, and expensive process. The recent groundbreaking development of several genome editing technologies has enabled the targeted alteration of almost any sequence in any cell or organism. These technologies have now been applied to mouse zygotes (in vivo genome editing), thereby providing new avenues for simple, convenient, and ultra-rapid production of knockout or knockin mice without the need for ES cells. Here, we review recent achievements in the production of gene-targeted mice by in vivo genome editing.
Laboratory of Molecular Neuroscience, Medical Research Institute, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-Ku, Tokyo, 113-8510, Japan.
Tomomi
T
Aida
Risa
R
Imahashi
Kohichi
K
Tanaka
D000818:Animals; D055879:Gene Knock-In Techniques / Q000379:methods; D018390:Gene Targeting / Q000379:methods*; D005818:Genetic Engineering / Q000379:methods*; D016678:Genome / Q000235:genetics*; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D013997:Time Factors; D015053:Zygote / Q000378:metabolism
clustered, regularly interspaced, short palindromic repeats- associated endonuclease; genome editing; mouse; transcription activator-like effector nucleases; zinc-finger nucleases