Detection of Escherichia fergusonii by PCR-based DNA sequencing in a case of delayed-onset chronic endophthalmitis after cataract surgery.
We report a case of chronic low-grade endophthalmitis after cataract surgery presenting with recurrent episodes of severe anterior chamber reactions and hypopyon uveitis caused by Escherichia fergusonii, which was isolated from vitreous aspirate by polymerase chain reaction-based DNA sequencing. Polymerase chain reaction has emerged as an essential, powerful, and rapid laboratory diagnostic technique and a useful adjunct to the conventional gold standard.


BACKGROUND
No author has a financial or proprietary interest in any material or method mentioned.
From the Uvea Department (Gokhale, Biswas), Medical Research Foundation, and L & T Microbiology Research Centre (Therese, Bagyalakshmi), Vision Research Foundation, Chennai, India. Electronic address: gokhale_varada@yahoo.co.in.
Varada Vinay
VV
Gokhale
From the Uvea Department (Gokhale, Biswas), Medical Research Foundation, and L & T Microbiology Research Centre (Therese, Bagyalakshmi), Vision Research Foundation, Chennai, India.
K Lily
KL
Therese
From the Uvea Department (Gokhale, Biswas), Medical Research Foundation, and L & T Microbiology Research Centre (Therese, Bagyalakshmi), Vision Research Foundation, Chennai, India.
R
R
Bagyalakshmi
From the Uvea Department (Gokhale, Biswas), Medical Research Foundation, and L & T Microbiology Research Centre (Therese, Bagyalakshmi), Vision Research Foundation, Chennai, India.
Jyotirmay
J
Biswas
D000368:Aged; D002908:Chronic Disease; D004269:DNA, Bacterial / Q000032:analysis*; D020878:Device Removal; D009877:Endophthalmitis / Q000175:diagnosis / Q000382:microbiology* / Q000601:surgery; D004756:Enterobacteriaceae Infections / Q000175:diagnosis / Q000382:microbiology* / Q000601:surgery; D004925:Escherichia / Q000235:genetics / Q000302:isolation & purification*; D015818:Eye Infections, Bacterial / Q000175:diagnosis / Q000382:microbiology* / Q000601:surgery; D005260:Female; D006801:Humans; D019654:Lens Implantation, Intraocular; D018918:Phacoemulsification; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014821:Vitrectomy; D014822:Vitreous Body / Q000382:microbiology
D004269:DNA, Bacterial