Chemical composition, and in vitro antibacterial and antifungal activity of an alkaloid extract from Crinum angustum Steud.
In the search for novel antibacterial agents against multidrug-resistant bacteria, an alkaloid extract obtained from whole plants of Crinum angustum Steud., containing six different groups of alkaloids, was analysed by using gas chromatography/mass spectrometry, and its in vitro activity against American Type Culture Collection bacterial strains and clinical isolates was evaluated. The antimicrobial activity of the extract was tested against nine standard strains of microorganisms and two drug-resistant clinical isolates, methicillin-resistant Staphylococcus aureus and carbapenemase-producing Klebsiella pneumoniae. The extract exhibited a significant activity against six of the examined strains of microorganisms. Antibacterial activity was more pronounced on Gram-positive bacteria than on Gram-negative bacteria, and IC50 values ranged from 156 to 625 μg/mL. Mycostatic activity against Candida albicans was also found, with an IC50 of 78 μg/mL after 48 h of incubation . Promising results were also obtained from tests carried out on both clinical isolates investigated.
a Department of Pharmacy and Biotechnology , University of Bologna , via Irnerio 42, 40137 Bologna , Italy.
Carmelina
C
Iannello
Jaume
J
Bastida
Francesca
F
Bonvicini
Fabiana
F
Antognoni
Giovanna Angela
GA
Gentilomi
Ferruccio
F
Poli
D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000378:metabolism; D002176:Candida albicans / Q000187:drug effects; D031407:Crinum / Q000737:chemistry*; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D020128:Inhibitory Concentration 50; D007558:Italy; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D001618:beta-Lactamases / Q000378:metabolism
D000470:Alkaloids; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase
Amaryllidaceae; Crinum angustum Steud; alkaloids; antimicrobial activity