APOBEC2 mRNA and protein is predominantly expressed in skeletal and cardiac muscles of chickens.
Apolipoprotein B mRNA-editing enzyme catalytic subunit 2 (APOBEC2) plays an important role in regulating and maintaining muscle development in mammals. In this study, we evaluated APOBEC2 mRNA abundance and protein expression and the results indicated that APOBEC2 mRNA was most abundant in skeletal and cardiac muscle, with relatively low expression in the gonads, gizzard and subcutaneous fat tissues of chickens. Immunoreactive APOBEC2 was localized to the cell nucleus of developing myocardium and skeletal myofibers. There were significant differences in mRNA and protein abundance among ages, tissues, and between males and females. In conclusion, APOBEC2 was expressed as the greatest in skeletal muscle and cardiac muscle where it localized to the nucleus. Thus, APOBEC2 may play an important role in muscle development in chickens.
College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan Province 625014, China; Research Institute of Raising Livestock, Chengdu Academy of Agriculture and Forestry Sciences, Chengdu, Sichuan Province 611130, China.
Juan
J
Li
College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan Province 625014, China.
Xiao-Ling
XL
Zhao
Department of Animal and Poultry Sciences 0306, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
Elizabeth Ruth
ER
Gilbert
College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan Province 625014, China.
Di-Yan
DY
Li
College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan Province 625014, China.
Yi-Ping
YP
Liu
College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan Province 625014, China.
Yan
Y
Wang
College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan Province 625014, China. Electronic address: zhuqingsicau@163.com.
Qing
Q
Zhu
College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan Province 625014, China.
Ya-Gang
YG
Wang
College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan Province 625014, China.
Yang
Y
Chen
College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan Province 625014, China.
Kai
K
Tian
D000071479:APOBEC-1 Deaminase; D000818:Animals; D015153:Blotting, Western; D002645:Chickens; D003564:Cytidine Deaminase / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D007124:Immunoenzyme Techniques; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D000071479:APOBEC-1 Deaminase; D003564:Cytidine Deaminase
APOBEC2; Chicken; Real-time PCR; Western blotting; mRNA/protein expression