Biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton's jelly in vitro.
Seeding cells and scaffolds play pivotal roles in bone tissue engineering and regenerative medicine. Wharton's jelly-derived mesenchymal stem cells (WJCs) from human umbilical cord represent attractive and promising seeding cells in tissue regeneration and engineering for treatment applications. This study was carried out to explore the biocompatibility of scaffolds to seeding cells in vitro. Rod-like nano-hydroxyapatite (RN-HA) and flake-like micro-hydroxyapatite (FM-HA) coatings were prepared on Mg-Zn-Ca alloy substrates using micro-arc oxidation and electrochemical deposition. WJCs were utilized to investigate the cellular biocompatibility of Mg-Zn-Ca alloys after different surface modifications by observing the cell adhesion, morphology, proliferation, and osteoblastic differentiation. The in vitro results indicated that the RN-HA coating group was more suitable for cell proliferation and cell osteoblastic differentiation than the FM-HA group, demonstrating better biocompatibility. Our results suggested that the RN-HA coating on Mg-Zn-Ca alloy substrates might be of great potential in bone tissue engineering.
School of Life Sciences, Zhengzhou University, Zhengzhou, 450001, China.
FangXia
F
Guan
ShanShan
S
Ma
XinYi
X
Shi
Xun
X
Ma
LianKai
L
Chi
Shuo
S
Liang
YuanBo
Y
Cui
ZhiBin
Z
Wang
Ning
N
Yao
ShaoKang
S
Guan
Bo
B
Yang
D000497:Alloys; D001483:Base Sequence; D001672:Biocompatible Materials; D002118:Calcium / Q000737:chemistry; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D017931:DNA Primers; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D008274:Magnesium / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures; D016133:Polymerase Chain Reaction; D013052:Spectrometry, X-Ray Emission; D054457:Tissue Scaffolds; D014470:Umbilical Cord / Q000166:cytology*; D015032:Zinc / Q000737:chemistry
D000497:Alloys; D001672:Biocompatible Materials; D017931:DNA Primers; D017886:Durapatite; D008274:Magnesium; D015032:Zinc; D002118:Calcium