[Changes in ion transport through membranes, ATPase activity and antibiotics effects in Enterococcus hirae after low intensity electromagnetic irradiation of 51,8 and 53,0 GHz frequencies].
It was ascertained that one-hour exposure of Enterococcus hirae ATCC9790 bacteria grown under anaerobe condition during sugar (glucose) fermentation to coherent electromagnetic irradiation (EMI) of 51,8 and 53,0 GHz frequencies or millimeter waves (5,79 and 5,66 mm wavelengths) of low-intensity (flux capacity of 0,06 mW/sm2) caused a significant decrease in energy-dependent H+ and K+ transports across the membranes of whole cells. Therewith, K+ influx into cells was appreciably less at the frequency of 53,0 GHz. Likewise, a significant decrease of total and N,N'-dicyclohexylcarbodiimide-sensitive ATPase activity of the membrane vesicles occurred after EMI of 51,8 and 53,0 GHz. These results indicated the input of membranous changes in bacterial action of low intensity extremely high frequency EMI, when the F0F1-ATPase was probably playing a key role. Additionally, the enhancement of the effects of antibiotics--ceftriaxone, kanamycin and ampicillin at their minimal inhibitory concentrations (100, 200 and 1,4 microM, correspondingly) on the bacterial growth by these irradiations was shown. Also, combined action of EMI and antibiotics depressed strongly H+ and K+ fluxes across membrane. Especially, H+ flux was more sensitive to the action of ceftriaxone, but K+ flux was sensitive to kanamycin. All these made the assumption that EMI of 51,8 and 53,0 GHz frequencies, especially 53,0 GHz, was followed by change in bacterial sensitivity toward antibiotics that was more obvious with ceftriaxone and ampicillin.
É
É
Torgomian
V
V
Oganian
C
C
Blbulian
A
A
Trchunian
D000667:Ampicillin / Q000494:pharmacology; D000693:Anaerobiosis; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002414:Cations, Monovalent; D002443:Ceftriaxone / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D060733:Electromagnetic Radiation; D016983:Enterococcus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D017136:Ion Transport / Q000187:drug effects / Q000528:radiation effects; D007612:Kanamycin / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D011188:Potassium / Q000378:metabolism; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011522:Protons
D000900:Anti-Bacterial Agents; D002414:Cations, Monovalent; D011522:Protons; D004024:Dicyclohexylcarbodiimide; D007612:Kanamycin; D002443:Ceftriaxone; D000667:Ampicillin; D006180:Proton-Translocating ATPases; D005947:Glucose; D011188:Potassium