Staphylococcus warneri, a resident skin commensal of rainbow trout (Oncorhynchus mykiss) with pathobiont characteristics.
Commensal microorganisms live in association with the mucosal surfaces of all vertebrates. The skin of teleost fish is known to harbor commensals. In this study we report for the first time the presence of an intracellular Gram positive bacteria, Staphylococcus warneri that resides in the skin epidermis of rainbow trout (Oncorhynchus mykiss). S. warneri was isolated from healthy hatchery trout skin epithelial cells. In situ hybridization confirmed the intracellular nature of the bacterium. Skin explants exposed in vitro to S. warneri or the extracellular pathogen Vibrio anguillarum show that S. warneri is able to induce an anti-inflammatory cytokine status via TGF-β1b compared to the pro-inflammatory responses (IL-1β, IL-6 and TNF-∝) elicited by V. anguillarum. In vivo experiments showed that S. warneri is not pathogenic to rainbow trout when injected intraperitoneally at high concentrations. However, S. warneri is able to stimulate V. anguillarum growth and biofilm formation on rainbow trout scales. Our results demonstrate that rainbow trout skin commensals such as S. warneri have the potential to become indirect pathobionts by enhancing growth and biofilm formation of pathogens such as V. anguillarum. These results show that fish farming practices (i.e. handling and other manipulations) can alter the skin microbiota and compromise the skin health of rainbow trout.
Center for Evolutionary and Theoretical Immunology, University of New Mexico, Albuquerque, NM, USA.
Rami
R
Musharrafieh
Center for Evolutionary and Theoretical Immunology, University of New Mexico, Albuquerque, NM, USA.
Luca
L
Tacchi
Center for Evolutionary and Theoretical Immunology, University of New Mexico, Albuquerque, NM, USA.
Joshua
J
Trujeque
Research Division, Clear Springs Food Inc., Buhl, ID, USA.
Scott
S
LaPatra
Center for Evolutionary and Theoretical Immunology, University of New Mexico, Albuquerque, NM, USA. Electronic address: isalinas@unm.edu.
Irene
I
Salinas
D000818:Animals; D058491:Bacterial Load; D016207:Cytokines / Q000378:metabolism; D004817:Epidermis / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D004847:Epithelial Cells / Q000382:microbiology; D029941:Fish Proteins / Q000378:metabolism; D007378:Interleukins / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D013210:Staphylococcus / Q000302:isolation & purification* / Q000472:pathogenicity / Q000502:physiology*; D013559:Symbiosis; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014733:Vibrio / Q000254:growth & development / Q000502:physiology*
D016207:Cytokines; D029941:Fish Proteins; D007378:Interleukins; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha
Pathobiont; Rainbow trout; Skin; Staphylococcus warneri; Vibrio anguillarum