Modification of the Miyake-Apple technique for simultaneous anterior and posterior video imaging of wet laboratory-based corneal surgery.
OBJECTIVE
The aim of this study was to describe a modification of the Miyake-Apple posterior video analysis for the simultaneous visualization of the anterior and posterior corneal surfaces during wet laboratory-based deep anterior lamellar keratoplasty (DALK).


METHODS
A human donor corneoscleral button was affixed to a microscope slide and placed onto a custom-made mounting box. A big bubble DALK was performed on the cornea in the wet laboratory. An 11-diopter intraocular lens was positioned over the aperture of the back camera of an iPhone. This served to video record the posterior view of the corneoscleral button during the big bubble formation. An overhead operating microscope with an attached video camcorder recorded the anterior view during the surgery.


RESULTS
The anterior and posterior views of the wet laboratory-based DALK surgery were simultaneously captured and edited using video editing software. The formation of the big bubble can be studied.


CONCLUSIONS
This video recording camera system has the potential to act as a valuable research and teaching tool in corneal lamellar surgery, especially in the behavior of the big bubble formation in DALK.
*Division of Cornea and External Disease, Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, BC, Canada; †National Healthcare Group Eye Institute, Tan Tock Seng Hospital, Singapore, Singapore; ‡Department of Ophthalmology and Visual Sciences, Surgery Teaching and Research Wet Laboratory, University of British Columbia, Vancouver, BC, Canada; and §South Australian Institute of Ophthalmology, Royal Adelaide Hospital, Adelaide, Australia.
Johnson C H
JC
Tan
Howard
H
Meadows
Aanchal
A
Gupta
Sonia N
SN
Yeung
Gregory
G
Moloney
D000388:Air; D003316:Corneal Diseases / Q000601:surgery; D016039:Corneal Transplantation / Q000379:methods; D006801:Humans; D007910:Lenses, Intraocular; D010781:Photography / Q000295:instrumentation; D014741:Video Recording / Q000379:methods*