Biotin-streptavidin enzyme-linked immunosorbent assay for detecting Tetrabromobisphenol A in electronic waste.
Tetrabromobisphenol A (TBBPA) is a widely used brominated flame retardant. A sensitive and selective indirect competitive biotin-streptavidin-amplified enzyme-linked immunosorbent assay (BA-ELISA) was developed for detecting TBBPA. The optimal hapten of TBBPA was 2-(2,6-dibromo-4-(2-(3,5-dibromo-4-hydroxyphenly)propan-2-yl)) acetic acid. Several physiochemical factors that influence assay performance, such as optimal coupling concentration of immunogen and antibody, organic solvent, ionic strength, and pH, were studied and optimized. The limit of detection (IC10) was 0.027 ng/mL and the median inhibitory concentration (IC50) was 0.58 ng/mL. The BA-ELISA was highly selective, with low cross-reactivity with TBBPA analogs. Finally, the assay was used to detect TBBPA in electronic waste samples. The results are consistent with those using liquid chromatography, which proves that the proposed immunoassay is accurate and receptive. This BA-ELISA method is suitable for the rapid and sensitive screening of TBBPA in environmental monitoring.
School of Environment Science and Technology, Shanghai Jiao Tong University, No. 800, Dongchuan Road, Minghang District, Shanghai 200240, PR China. Electronic address: budan198603@163.com.
Dan
D
Bu
School of Environment Science and Technology, Shanghai Jiao Tong University, No. 800, Dongchuan Road, Minghang District, Shanghai 200240, PR China. Electronic address: huishengzhuang@126.com.
Huisheng
H
Zhuang
School of Agriculture and Biology, Shanghai Jiao Tong University, No. 2678, Qixin Road, Minghang District, Shanghai 201101, PR China. Electronic address: zxc_02622@sina.com.
Xinchu
X
Zhou
School of Environment Science and Technology, Shanghai Jiao Tong University, No. 800, Dongchuan Road, Minghang District, Shanghai 200240, PR China. Electronic address: yangpai0717@163.com.
Guangxin
G
Yang
D001710:Biotin / Q000737:chemistry; D059029:Electronic Waste / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005411:Flame Retardants / Q000032:analysis*; D057230:Limit of Detection; D011075:Polybrominated Biphenyls / Q000032:analysis*; D019809:Streptavidin / Q000737:chemistry
D005411:Flame Retardants; D011075:Polybrominated Biphenyls; D001710:Biotin; D019809:Streptavidin; C020806:tetrabromobisphenol A
Biotin–streptavidin-amplified system; Electronic waste; Enzyme-linked immunosorbent assay; Tetrabromobisphenol A