Characterization of the aap1 gene of Agaricus bisporus, a homolog of the yeast YAP1.
The structure, homologies, polymorphism and expression profiles of a new gene, aap1, have been studied for precisely characterizing it and defining its putative involvement in thermo-tolerance of both vegetative mycelium growth and sporophore differentiation. Sequence polymorphism was analyzed in 3 homokaryons of A. bisporus and 24 strains having different abilities for mycelial growth at temperatures above 30 °C and for producing mature fruiting bodies at 25 °C. The level of gene expression was measured by real-time PCR both in vegetative mycelium after transfer from 25 to 32 °C and in primordia and fruiting bodies produced during cultures at 17 or 25 °C. The results indicated that aap1 gene belong to a new subfamily of the yeast YAP1 homologs. It is not a dominant contributor to the thermo-tolerance of A. bisporus, but the protein it encodes may be involved as an overall stress resistance transcription factor. The way Aap1 senses redox level differs from that of AP-1-like transcription factor Yap1.
INRA, UR1264 MycSA, CS 20032, 33882 Villenave-d'Ornon cedex, France; Universidad Veracruzana, Facultad de Ciencias Biológicas y Agropecuarias, Córdoba, Veracruz, Mexico. Electronic address: pilarn@unam.mx.
Pilar
P
Navarro
INRA, UR1264 MycSA, CS 20032, 33882 Villenave-d'Ornon cedex, France. Electronic address: billette@bordeaux.inra.fr.
Christophe
C
Billette
INRA, UR1264 MycSA, CS 20032, 33882 Villenave-d'Ornon cedex, France. Electronic address: nferrer@bordeaux.inra.fr.
Nathalie
N
Ferrer
INRA, UR1264 MycSA, CS 20032, 33882 Villenave-d'Ornon cedex, France. Electronic address: savoie@bordeaux.inra.fr.
Jean-Michel
JM
Savoie
D000364:Agaricus / Q000235:genetics*; D000595:Amino Acid Sequence; D004271:DNA, Fungal / Q000235:genetics; D048690:Fruiting Bodies, Fungal / Q000235:genetics / Q000502:physiology; D005656:Fungal Proteins / Q000235:genetics* / Q000502:physiology; D005800:Genes, Fungal / Q000502:physiology*; D006358:Hot Temperature; D008969:Molecular Sequence Data; D025282:Mycelium / Q000502:physiology; D011110:Polymorphism, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000502:physiology; D013172:Spores, Fungal / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology; D014158:Transcription, Genetic / Q000502:physiology
D004271:DNA, Fungal; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C086677:YAP1 protein, S cerevisiae
AP-1-like transcription factor; Adaptation à la température; Champignon de couche; Facteur de transcription Yap; Facteur de transcription de type AP-1; Mushroom; Temperature adaptation; Yap transcription factor; bZIP