Structure elucidation and immunomodulatory activity in vitro of a xylan from roots of Cudrania tricuspidata.
To discover new immunomodulator in the food industry, a water-soluble polysaccharide, CTPB1 was obtained from roots of Cudrania tricuspidata (Carr.) Bur. The average molecular weight of this polysaccharide was estimated to be 4.0×10(4) Da. Monosaccharide composition analysis indicated that the only neutral sugar present in this polysaccharide was xylose. From methylation analysis and (1)H and (13)C NMR spectroscopy, the structure of CTPB1 was deduced to be (4-O-methyl-D-glucurono)-D-xylan, with the 4-O-methyl-D-glucopyranosyluronic acid group linked to O-2 of a (1→4)-β-D-xylan. The repeating unit of CTPB1 is composed of one non-reducing terminal residue of 4-O-methyl-D-glucuronic acid on every four xylose residues. Immunomodulatory activity assays in vitro showed that CTPB1 may induce the proliferation of mouse splenocytes. It was suggested that CTPB1 could be a potential immunostimulant used in the food industry.
College of Life Science, Qufu Normal University, Qufu 273165, China; Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, China; Temasek Applied Science School, Temasek Polytechnic, 21 Tampines Avenue 1, 529757 Singapore, Singapore. Electronic address: slsql2006@aliyun.com.
Lei
L
Shi
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, China.
Qun
Q
Dong
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, China. Electronic address: kding@mail.shcnc.ac.cn.
Kan
K
Ding
D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D007155:Immunologic Factors / Q000737:chemistry* / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D029586:Moraceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry*; D013154:Spleen / Q000166:cytology; D014990:Xylans / Q000737:chemistry* / Q000494:pharmacology
D007155:Immunologic Factors; D010936:Plant Extracts; D014990:Xylans
Cudrania tricuspidata; Immunomodulatory activity; Purification; Structure; Xylan