[Determination of ethylene glycol in biological fluids--propylene glycol interferences].
Many laboratories in Poland do not use gas chromatography (GC) method for determination of ethylene glycol (EG) and methanol in blood of poisoned patients, they use non specific spectrophotometry methods. One of the interfering substances is propylene glycol (PG)--compound present in many medical and cosmetic products: drops, air freshens, disinfectants, electronic cigarettes and others. In Laboratory of Analytical Toxicology and Drug Monitoring in Krakow determination of EG is made by GC method. The method enables to distinguish and make resolution of (EG) and (PG) in biological samples. In the years 2011-2012 in several serum samples from diagnosed patients PG was present in concentration from several to higher than 100 mg/dL. The aim of the study was to estimate PG interferences of serum EG determination by spectrophotometry method. Serum samples containing PG and EG were used in the study. The samples were analyzed by two methods: GC and spectrophotometry. Results of serum samples spiked with PG with no EG analysed by spectrophotometry method were improper ("false positive"). The results were correlated to PG concentration in samples. Calculated cross-reactivity of PG in the method was 42%. Positive results of EG measured by spectrophotometry method must be confirmed by reference GC method. Spectrophotometry method shouldn't be used for diagnostics and monitoring of patients poisoned by EG.
Pracownia Toksykologii Analitycznej i Terapii Monitorowanej, Katedra Toksykologii i Chorób Srodowiskowych, Uniwersytet Jagielloński Collegium Medicum w Krakowie. ewa.gomolka@uj.edu.pl
Ewa
E
Gomółka
Pracownia Toksykologii, Zakład Diagnostyki Szpital Uniwersytecki w Krakowie.
Sylwia
S
Cudzich-Czop
Pracownia Toksykologii Analitycznej i Terapii Monitorowanej, Katedra Toksykologii i Chorób Srodowiskowych, Uniwersytet Jagielloński Collegium Medicum w Krakowie.
Adrianna
A
Sulka
D019855:Ethylene Glycol / Q000097:blood* / Q000737:chemistry / Q000652:urine; D006801:Humans; D019946:Propylene Glycol / Q000097:blood* / Q000737:chemistry / Q000652:urine; D015203:Reproducibility of Results; D013053:Spectrophotometry
D019946:Propylene Glycol; D019855:Ethylene Glycol