[Transformation of 2- and 4-cyanopyridines by free and immobilized cells of nitrile-hydrolyzing bacteria].
The transformation dynamics of 2- and 4-cyanopyridines by cells suspended and adsorbed on inorganic carriers has been studied in the Rhodococcus ruber gt 1 strain possessing nitrile hydratase activity and the Pseudomonas fluorescens C2 strain containing nitrilase. It was shown that both nitrile hydratase and nitrilase activities of immobilized cells against 2-cyanopyridine were 1.5-4 times lower compared to 4-cyanopyridine and 1.6-2 times lower than the activities of free cells against 2-cyanpopyridine. The possibility of obtaining isonicotinic acid during the combined conversion of 4-cyanopyridine by a mixed suspension of R. ruber gt 1 cells with a high level of nitrile hydratase activity and R. erythropolis 11-2 cells with a pronounced activity of amidase has been shown. Immobilization of Rhodococcus cells on raw coal and Pseudomonas cells on china clay was shown to yield a heterogeneous biocatalyst for the efficient transformation of cyanopyridines into respective amides and carbonic acids.
Iu G
IuG
Maksimova
D M
DM
Vasil'ev
G V
GV
Ovechkina
A Iu
AIu
Maksimov
V A
VA
Demakov
D000538:Aluminum Silicates / Q000737:chemistry; D000619:Aminohydrolases / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D001711:Biotransformation; D018914:Cells, Immobilized; D000077215:Clay; D003031:Coal; D006836:Hydro-Lyases / Q000378:metabolism*; D007539:Isonicotinic Acids / Q000378:metabolism; D007700:Kinetics; D009570:Nitriles / Q000378:metabolism*; D011551:Pseudomonas fluorescens / Q000378:metabolism*; D011725:Pyridines / Q000378:metabolism*; D012240:Rhodococcus / Q000378:metabolism*
D000538:Aluminum Silicates; D001426:Bacterial Proteins; D003031:Coal; D007539:Isonicotinic Acids; D009570:Nitriles; D011725:Pyridines; D000619:Aminohydrolases; C021867:nitrilase; D006836:Hydro-Lyases; C048831:nitrile hydratase; D000077215:Clay