Investigation of chemokine receptor CCR2V64Il gene polymorphism and migraine without aura in the Iranian population.
OBJECTIVE
Migraine is a multifactorial common neurovascular disease with a polygenic inheritance. Inflammation plays an important part in migraine pathophysiology. C-C chemokine receptor 2 (CCR2) is an important chemokine for monocyte aggregation and transendothelial monocyte migration. The aim of our study was to investigate the association of migraine with CCR2V64Il polymorphism in the Iranian population.


METHODS
We assessed 103 patients with newly diagnosed migraine and 100 healthy subjects. Genomic DNA samples were extracted from peripheral blood and genotypes of CCR2V64Il gene polymorphism were determined. For measuring the severity of headache, every patient filled out the MIGSEV questionnaire.


RESULTS
There were no significant differences in the distribution of both 64Il allele and heterozygote (GA) genotype of CCR2 gene polymorphism (P = 0.396; OR = 0.92, 95% CI = 0.50-1.67 and P = 0.388; OR = 0.91, 95% CI = 0.47-1.73, resp.) between case and control groups. There was no significant difference of alleles frequency between three grades of MIGSEV (P = 0.922).


CONCLUSIONS
In conclusion our results revealed no association between CCR2V64Il polymorphism and susceptibility to migraine and also headache severity in the Iranian population.
Medical Student Research Center, Isfahan University of Medical Sciences, Isfahan 81745-319, Iran ; Physiology Research Center, Department of Physiology, Isfahan University of Medical Sciences, Isfahan 81745-319, Iran.
Alireza
A
Zandifar
Medical Student Research Center, Isfahan University of Medical Sciences, Isfahan 81745-319, Iran ; Physiology Research Center, Department of Physiology, Isfahan University of Medical Sciences, Isfahan 81745-319, Iran.
Maryam
M
Taheriun
Medical Student Research Center, Isfahan University of Medical Sciences, Isfahan 81745-319, Iran ; Physiology Research Center, Department of Physiology, Isfahan University of Medical Sciences, Isfahan 81745-319, Iran.
Samira
S
Soleimani
Medical Student Research Center, Isfahan University of Medical Sciences, Isfahan 81745-319, Iran ; Physiology Research Center, Department of Physiology, Isfahan University of Medical Sciences, Isfahan 81745-319, Iran.
Faraidoon
F
Haghdoost
Physiology Research Center, Department of Physiology, Isfahan University of Medical Sciences, Isfahan 81745-319, Iran ; School of Pharmacy and Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan 81745-319, Iran.
Mohamadhasan
M
Tajaddini
Physiology Research Center, Department of Physiology, Isfahan University of Medical Sciences, Isfahan 81745-319, Iran.
Shaghayegh Haghjooy
SH
Javanmard
D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D004252:DNA Mutational Analysis; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007492:Iran / Q000453:epidemiology; D008297:Male; D020326:Migraine without Aura / Q000453:epidemiology / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D054390:Receptors, CCR2 / Q000235:genetics* / Q000502:physiology; D012720:Severity of Illness Index; D012959:Socioeconomic Factors; D011795:Surveys and Questionnaires
C515334:CCR2 protein, human; D054390:Receptors, CCR2