An aptamer-based electrochemical biosensor for the detection of Salmonella.
Salmonella is one of the most common causes of food-associated disease. An electrochemical biosensor was developed for Salmonella detection using a Salmonella-specific recognition aptamer. The biosensor was based on a glassy carbon electrode modified with graphene oxide and gold nanoparticles. Then, the aptamer ssDNA sequence could be linked to the electrode. Each assembly step was accompanied by changes to the electrochemical parameters. After incubation of the modified electrode with Salmonella, the electrochemical properties between the electrode and the electrolyte changed accordingly. The electrochemical impedance spectrum was measured to quantify the Salmonella. The results revealed that, when more Salmonella were added to the reaction system, the current between the electrode and electrolyte decreased; in other words, the impendence gradually increased. A detection limit as low as 3 cfu/mL was obtained. This novel method is specific and fast, and it has the potential for real sample detection.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, 214122, China.
Xiaoyuan
X
Ma
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Yihui
Y
Jiang
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Fei
F
Jia
Zhangjiagang Entry-Exit Inspection and Quarantine Bureau, Zhangjiagang, 215600, China.
Ye
Y
Yu
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Jie
J
Chen
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, 214122, China. Electronic address: wangzp@jiangnan.edu.cn.
Zhouping
Z
Wang
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D002244:Carbon / Q000737:chemistry; D017097:Electric Impedance; D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D005516:Food Microbiology / Q000295:instrumentation* / Q000379:methods; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D057230:Limit of Detection; D008460:Meat / Q000382:microbiology; D053768:Metal Nanoparticles / Q000737:chemistry; D012475:Salmonella / Q000737:chemistry*
D052157:Aptamers, Nucleotide; D002244:Carbon; D006046:Gold; D006108:Graphite
Aptamer; Electrochemical detection; Gold nanoparticles; Graphene oxide; Salmonella