HDX-MS takes centre stage at unravelling kinase dynamics.
In recent years, HDX-MS (hydrogen-deuterium exchange coupled to MS) on biomolecules has evolved from a niche technique to a powerful method in the investigation of protein dynamics. Protein kinases, in particular, represent an area of active study using this technique owing to their well-characterized protein structures and their relevance to diseases such as cancer, immune disorders and neurodegenerative defects. In the present review, we describe how HDX-MS has revealed important dynamic properties of protein kinases and provided insight into the mechanisms of drug binding.
*Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, ON, Canada, M5G 1X5.
Kristina
K
Lorenzen
*Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, ON, Canada, M5G 1X5.
Tony
T
Pawson
D020134:Catalytic Domain; D041961:Deuterium Exchange Measurement; D004789:Enzyme Activation; D057075:Enzyme Assays; D006801:Humans; D013058:Mass Spectrometry; D010766:Phosphorylation; D011485:Protein Binding; D047428:Protein Kinase Inhibitors / Q000737:chemistry / Q000494:pharmacology; D011494:Protein Kinases / Q000737:chemistry* / Q000502:physiology; D011499:Protein Processing, Post-Translational
D047428:Protein Kinase Inhibitors; D011494:Protein Kinases