Selective miRNA expression profile in chronic myeloid leukemia K562 cell-derived exosomes.
BACKGROUND
Chronic myeloid leukemia (CML) is a myeloproliferative disorder of hematopoietic stem cell scarrying the Philadelphia (Ph) chromosome and an oncogenic BCR-ABL1 fusion gene. The tyrosine kinase inhibitor (TKI) of BCR-ABL1 kinase is a treatment of choice for control of CML.


OBJECTIVE
Recent studies have demonstrated that miRNAs within exosomes from cancer cells play crucial roles in initiation and progression. This study was performed to assess miRNAs within exosomes of K562 cells.


METHODS
miRNA microarray analysis of K562 cells and K562 cell-derived exosomes was conducted with the 6th generation miRCURYTM LNA Array (v.16.0). Gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were also carried out. GO terms and signaling pathways were categorized into 66 classes (including homophilic cell adhesion, negative regulation of apoptotic process, cell adhesion) and 26 signaling pathways (such as Wnt).


RESULTS
In exosomes, 49 miRNAs were up regulated as compared to K562 cells, and two of them were further confirmed by quantitative real-time PCR. There are differentially expressed miRNAs between K562 cell derived-exosomes and K562 cells.


CONCLUSIONS
Selectively expressed miRNAs in exosomes may promote the development of CML via effects on interactions (e.g. adhesion) of CML cells with their microenvironment.
Department of Clinical Laboratory, the Second Affiliated Hospital of Nanchang University, Nanchang, China E-mail : wangxiaozhong@ncu.edu.cn.
Dan-Qin
DQ
Feng
Bo
B
Huang
Jing
J
Li
Jing
J
Liu
Xi-Min
XM
Chen
Yan-Mei
YM
Xu
Xin
X
Chen
Hai-Bin
HB
Zhang
Long-Hua
LH
Hu
Xiao-Zhong
XZ
Wang
D014408:Biomarkers, Tumor / Q000235:genetics*; D015153:Blotting, Western; D002448:Cell Adhesion / Q000235:genetics*; D049109:Cell Proliferation; D055354:Exosomes / Q000235:genetics*; D020869:Gene Expression Profiling; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D035683:MicroRNAs; D012333:RNA, Messenger