[Exploring the relationship between secreted frizzled-related protein 1 and chronic periodontitis].
OBJECTIVE
This study detects the expression of secreted frizzled-related protein 1 (SFRP1) in healthy patients and patients with chronic periodontitis (CP) and explores the relationship between SFRP1 and the occurrence and development of CP.


METHODS
First, 28 patients forming the CP group were further divided into mild, moderate, and severe CP subgroups according to clinical attachment loss (CAL) data. Ten healthy volunteers were recruited in the control group. Gingival crevicular fluid (GCF) was collected from all of the patients, and the concentration of SFRP1 in the GCF samples was detected using enzyme-linked immunosorbent assay. Next, gingival lesions were obtained from 22 patients in the CP group and healthy gingival tissues were obtained from the 10 healthy patients in the control group. Immunohistochemical analysis for SFRP1 was used to analyze the correlation between the expression of SFRP1 and the severity of CP based on staining intensities.


RESULTS
The concentration of SFRP1 in GCF samples taken from of the CP group (281.07 ng x L(-1) +/- 33.37 ng x L(-1)) was significantly higher than that in samples taken from the control group (245.30 ng x L(-1) +/- 35.69 ng x L(-1)) (P < 0.05). A significant positive correlation was observed between the concentration of SFRP1 in GCF and CAL (r = 0.651, P < 0.001). Furthermore, the SFRP1 scores in the CP groups (4.500 +/- 0.913) were significantly higher than those in the control group (2.800 +/- 1.135) (P < 0.001). SFRP1 scores did not vary significantly among the CP subgroups (P > 0.05).


CONCLUSIONS
SFRP1 expression in the CP groups was significantly higher than that in the control group. Thus, SFRP1 may play a significant role in the development of CP.
Dept. of Stomatology, Xiangya Hospital of Central South University, Changsha 410008, China.
Haibo
H
Yuan
Dept. of Stomatology, Xiangya Hospital of Central South University, Changsha 410008, China.
Jing
J
Jin
Dept. of Stomatology, Xiangya Hospital of Central South University, Changsha 410008, China.
Chunjiao
C
Xu
Dept. of Periodontology and Oral Medicine, School of Stomatology, Central South University, Changsha 410078, China.
Xili
X
Qiu
Dept. of Periodontology and Oral Medicine, School of Stomatology, Central South University, Changsha 410078, China.
Lu
L
Wang
Dept. of Periodontology and Oral Medicine, School of Stomatology, Central South University, Changsha 410078, China.
Fengyuan
F
Lü
Dept. of Periodontology and Oral Medicine, School of Stomatology, Central South University, Changsha 410078, China.
Tingting
T
Liu
Dept. of Stomatology, Xiangya Hospital of Central South University, Changsha 410008, China.
Yingfang
Y
Wu
Dept. of Stomatology, Xiangya Hospital of Central South University, Changsha 410008, China.
Xiaomin
X
Yin
Dept. of Stomatology, Xiangya Hospital of Central South University, Changsha 410008, China.
Changyun
C
Fang
Dept. of Stomatology, Xiangya Hospital of Central South University, Changsha 410008, China.
Guoying
G
Que
Dept. of Stomatology, Xiangya Hospital of Central South University, Changsha 410008, China.
Jieying
J
Peng
D055113:Chronic Periodontitis; D005881:Gingiva; D005883:Gingival Crevicular Fluid; D006023:Glycoproteins; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D010512:Periodontal Index; D010518:Periodontitis; D011506:Proteins; D000099274:Frizzled-Related Proteins
D006023:Glycoproteins; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; D000099274:Frizzled-Related Proteins