In vitro osteogenic cell proliferation, mineralization, and in vivo osseointegration of injection molded high-density polyethylene-based hybrid composites in rabbit animal model.
The present work reports the biocompatibility property of injection molded HDPE-HA-Al2O3 hybrid composites. In vitro cytocompatibility results reveal that osteogenic cell viability and bone mineralization are favorably supported in a statistically significant manner on HDPE-20% HA-20% Al2O3 composite, in comparison to HDPE-40 wt.% HA or HDPE-40 wt.% Al2O3 The difference in cytocompatibility property is explained in terms of difference in substrate wettability/surface energy and importantly, both the cell proliferation at 7 days or bone mineralization at 21 days on HDPE-20% HA-20% Al2O3 composite are either comparable or better than sintered HA. The progressive healing of cylindrical femoral bone defects in rabbit animal model was assessed by implantation experiments over 1, 4 and 12 weeks. Based on the histological analysis as well as histomorphometrical evaluation, a better efficacy of HDPE-20% HA-20% Al2O3 over high-density polyethylene (HDPE) for bone regeneration and neobone formation at host bone-implant interface was established. Taken together, the present study unequivocally establishes that despite the presence of 20% Al2O3, HDPE-based hybrid composites are as biocompatible as HA in vitro or better than HDPE in vivo.
Department of Materials Science and Engineering, Indian Institute of Technology Kanpur, Kanpur, India.
Garima
G
Tripathi
Laboratory for Biomaterials, Materials Research Centre, Indian Institute of Science, Bangalore, India bikram@mrc.iisc.ernet.in.
Bikramjit
B
Basu
D000537:Aluminum Oxide / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D018786:Bone Substitutes / Q000737:chemistry*; D002113:Calcification, Physiologic; D002448:Cell Adhesion; D049109:Cell Proliferation; D002470:Cell Survival; D017886:Durapatite / Q000737:chemistry; D020007:Implants, Experimental; D066298:In Vitro Techniques; D008422:Materials Testing; D016348:Osseointegration; D010006:Osteoblasts / Q000166:cytology; D010012:Osteogenesis; D020959:Polyethylene / Q000737:chemistry; D011817:Rabbits
D001672:Biocompatible Materials; D018786:Bone Substitutes; D020959:Polyethylene; D017886:Durapatite; D000537:Aluminum Oxide
Surface property; cell adhesion; histopathology; in vivo implantation