Development of 116 kDa Fraction for Detecting Experimental Toxoplasma gondii Infections in Mice.
BACKGROUND
Serological diagnosis of Toxoplasma gondii infection using crude antigens may not be more accurate. To increase the diagnostic potency of antigens, isolation of their immunogenic fractions could be useful. The current research adopted to obtain an affinity isolated fraction from RH strain using CNBr Sepharose 4B column coupled with infected mice sera helping in detection of IgM and IgG of toxoplasmosis due to RH strain and other strains.


METHODS
The isolated fraction was characterized by SDS-PAGE. Moreover, the diagnostic potency of the fraction was assessed by indirect ELISA in mice experimentally infected with RH strain and two other local strains; one of sheep origin and the other of human origin.


RESULTS
The fraction was found to be consisted of a single band of 116 kDa compared with 17 bands ranged from 116 to 16 kDa associated with crude extract. The fraction proved potent diagnostic potentials of acute and chronic mice toxoplasmosis. Where it was detected both IgM and IgG antibodies as early as two days and as late as 2 months post experimental infection with any of the three strains. The level of detected IgM and IgG by RH fraction was higher in mice infected with RH strain than with local strains except IgM due to sheep strain parasite.


CONCLUSIONS
The 116 kDa fraction of T. gondii tachyzoites can be considered as a candidate in improving of serodiagnosisof Toxoplasma infections.
Department of Zoonosis, Veterinary Research Division, National Research Center, Dokki, Giza, Egypt.
Mohey Abdel-Hafez
MA
Hassanain
Department of Parasitology and Animal Diseases, Veterinary Research Division, National Research Center, Dokki, Giza, Egypt.
Eman Hussien
EH
Abdel-Rahman
Department of Parasitology and Animal Diseases, Veterinary Research Division, National Research Center, Dokki, Giza, Egypt.
Nagwa Ibrahim
NI
Toaleb
Department of Zoonosis, Veterinary Research Division, National Research Center, Dokki, Giza, Egypt.
Raafat Mohamed
RM
Shaapan
Department of Zoonosis, Veterinary Research Division, National Research Center, Dokki, Giza, Egypt.
Hasan Ali
HA
Elfadaly
Department of Zoonosis, Veterinary Research Division, National Research Center, Dokki, Giza, Egypt.
Nawal Abdel-Hafez
NA
Hassanain
ELISA; Mice; Partially purified fraction; Strains; Toxoplasma gondii