Protein and enzyme gated supramolecular disassembly.
An amphiphilic nanoassembly was designed to respond to the concurrent presence of a protein and an enzyme. We present herein a system, where in the presence of these two stimuli supramolecular disassembly and molecular release occur. This molecular release arises in the form a fluorescence response that has been shown to be specific. We also show that this system can be modified to respond only if light stimulus is also present in addition to the protein and the enzyme. Demonstration of such supramolecular disassembly principles could have broad implications in a variety of biological applications.
Department of Chemistry, University of Massachusetts , Amherst, Massachusetts 01003, United States.
Jing
J
Guo
Jiaming
J
Zhuang
Feng
F
Wang
Krishna R
KR
Raghupathi
S
S
Thayumanavan
D001665:Binding Sites; D003374:Coumarins / Q000737:chemistry / Q000494:pharmacology; D004798:Enzymes / Q000737:chemistry* / Q000187:drug effects; D007202:Indicators and Reagents / Q000494:pharmacology; D008954:Models, Biological; D015394:Molecular Structure; D021381:Protein Transport; D011506:Proteins / Q000737:chemistry*; D013379:Substrate Specificity
D003374:Coumarins; D004798:Enzymes; D007202:Indicators and Reagents; D011506:Proteins; C030123:coumarin