Expression of glucosylceramide synthase in invasive ductal breast cancer may be correlated with high estrogen receptor status and low HER-2 status.
OBJECTIVE
Breast cancer is one of the most common causes of cancer-related deaths in women worldwide. Studies on glucosylceramide synthase (GCS) activity suggest that this enzyme has a role in the development of multidrug resistance in many cancer cells. However, few studies have shown the expression of GCS in invasive ductal breast cancer and breast intraductal proliferative lesions.


METHODS
In total, 196 samples from patients with invasive ductal breast cancer and 61 samples of breast intraductal proliferative lesions were collected. Immunohistochemical analyses were conducted to determine the expression of GCS and other related proteins.


RESULTS
Expression of GCS was high in estrogen receptor (ER)-positive and HER-2 negative samples. In contrast, the expression of GCS in invasive ductal cancer was significantly lower than that in intraductal proliferative lesions.


CONCLUSIONS
Our data demonstrates a correlation between the expression of the GCS protein and ER-positive/HER-2 negative breast cancer. Furthermore, in contrast to previous reports, the expression of GCS protein was shown to be much higher in ductal carcinoma in-situ than that in invasive ductal cancer.
The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1559854430111589.
Jiannan
J
Liu
Ping
P
Sun
Yuan
Y
Sun
Aina
A
Liu
Dong
D
You
Fenge
F
Jiang
Department of Oncology, Jinan Central Hospital, Affiliated to Shandong University, Jinan, Shandong 250013, P R China. sunyuping@live.cn.
Yuping
Y
Sun
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis; D001943:Breast Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000401:mortality; D018270:Carcinoma, Ductal, Breast / Q000201:enzymology* / Q000235:genetics / Q000401:mortality; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000201:enzymology / Q000235:genetics / Q000401:mortality; D005260:Female; D005964:Glucosyltransferases / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008875:Middle Aged; D011379:Prognosis; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D011960:Receptors, Estrogen / Q000032:analysis / Q000235:genetics / Q000378:metabolism
D014408:Biomarkers, Tumor; D005964:Glucosyltransferases; D018719:Erb-b2 Receptor Tyrosine Kinases; D011960:Receptors, Estrogen; C023246:ceramide glucosyltransferase; C508053:ERBB2 protein, human