Screening of α-glucosidase inhibitors from green tea extracts using immobilized enzymes affinity capture combined with UHPLC-QTOF MS analysis.
An applicable affinity capture-based method was developed for screening of enzyme inhibitors from complex plant extracts directly. By elimination of false positives, three non-competitive α-glucosidase inhibitors were fished out from 400 μL green tea extract rapidly, using affinity capture of immobilized α-glucosidase combined with UHPLC-QTOF MS analysis.
Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. hbxiao@dicp.ac.cn.
Shiren
S
Deng
Linbo
L
Xia
Hongbin
H
Xiao
D001667:Binding, Competitive; D002851:Chromatography, High Pressure Liquid; D004353:Drug Evaluation, Preclinical; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D004800:Enzymes, Immobilized; D065089:Glycoside Hydrolase Inhibitors; D013058:Mass Spectrometry; D015394:Molecular Structure; D010936:Plant Extracts / Q000378:metabolism* / Q000494:pharmacology*; D011485:Protein Binding / Q000187:drug effects; D013662:Tea / Q000737:chemistry*
D004791:Enzyme Inhibitors; D004800:Enzymes, Immobilized; D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts; D013662:Tea