Short communication: subtyping of Mycobacterium kansasii by PCR-restriction enzyme analysis of the hsp65 gene.
Mycobacterium kansasii is one of the most common causes of pulmonary disease resulting from nontuberculous mycobacteria (NTM). It is also the most frequently isolated NTM species from clinical specimens in Poland. The aim of this study was to investigate the distribution of M. kansasii subtypes among patients suspected of having pulmonary NTM disease. Fifty clinical isolates of M. kansasii recovered from as many patients with suspected mycobacterial lung disease between 2000 and 2010 in Poland were genotyped by PCR-restriction enzyme analysis (PCR-REA) of partial hsp65 gene. Mycobacterium kansasii subtype I was the only genotype to be identified among the isolates, both disease-associated and non-disease-associated. Isolation of M. kansasii subtype I from clinical specimens may be indicative of infection but may also merely represent colonization.
Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, I. Miecznikowa 1, 02-096 Warsaw, Poland.
Zofia
Z
Bakuła
Department of Internal Medicine, Pneumonology, and Allergology, Medical University of Warsaw, Żwirki i Wigury 61, 02-091 Warsaw, Poland.
Aleksandra
A
Safianowska
Clinic of Internal Medicine, Pneumonology, and Allergology, Independent Public Central Clinical Hospital, S. Banacha 1A, 02-097 Warsaw, Poland.
Magdalena
M
Nowacka-Mazurek
Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, I. Miecznikowa 1, 02-096 Warsaw, Poland.
Jacek
J
Bielecki
Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, I. Miecznikowa 1, 02-096 Warsaw, Poland.
Tomasz
T
Jagielski
D001426:Bacterial Proteins / Q000235:genetics*; D018834:Chaperonin 60 / Q000235:genetics*; D006801:Humans; D009165:Mycobacterium Infections, Nontuberculous / Q000235:genetics / Q000382:microbiology*; D019909:Mycobacterium kansasii / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D016133:Polymerase Chain Reaction; D000091402:Prohibitins; D015183:Restriction Mapping
D001426:Bacterial Proteins; D018834:Chaperonin 60; C000717331:PHB2 protein, human; D000091402:Prohibitins; C074119:heat-shock protein 65, Mycobacterium