Allergenicity of an enzymatic hydrolysate of soybean 2S protein.
BACKGROUND
This study was performed to examine how the characteristics of soybean 2S protein influence allergenicity after enzymatic hydrolysis. Soybean 2S protein was extracted and enzymatic hydrolysis was performed using pepsin and chymotrypsin. Allergenicity was observed using soybean-sensitive patients' sera.


RESULTS
Only 13.3% (6/45) of soybean-sensitive patients reacted to soybean Kunitz trypsin inhibitor (SKTI), known as the major allergen of soybean 2S protein. After peptic hydrolysis for 90 min at pH 1.2, the intensity of SKTI decreased to 25% but was still visible on SDS-PAGE. Chymotryptic hydrolysis following peptic hydrolysis at pH 8 for 60 min showed a limited hydrolytic effect on soybean 2S protein. Peptic hydrolysis of soybean 2S protein partially reduced the allergenicity of soybean 2S protein, while chymotryptic hydrolysis following peptic hydrolysis increased slightly the allergenicity.


CONCLUSIONS
Food allergy caused by soybean 2S protein occurred in part of the soybean-sensitive patients. SKTI was partially digested after peptic hydrolysis for 90 min. The allergenicity was decreased with peptic hydrolysis, while subsequent treatment of chymotrypsin increased slightly the allergenicity.
Department of Food Science and Technology, College of Engineering, Ewha Womans University, Seoul, Korea.
Dongeun
D
Sung
Kang Mo
KM
Ahn
Seung-Yong
SY
Lim
Sangsuk
S
Oh
D000485:Allergens / Q000276:immunology*; D002675:Child, Preschool; D002918:Chymotrypsin / Q000378:metabolism*; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology*; D006801:Humans; D006868:Hydrolysis; D007223:Infant; D008297:Male; D010434:Pepsin A / Q000378:metabolism*; D011492:Protein Hydrolysates / Q000276:immunology* / Q000378:metabolism; D030262:Soybean Proteins; D013025:Glycine max / Q000737:chemistry*; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000276:immunology* / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000276:immunology
D000485:Allergens; D011492:Protein Hydrolysates; D030262:Soybean Proteins; D014361:Trypsin Inhibitors; D014360:Trypsin Inhibitor, Kunitz Soybean; D002918:Chymotrypsin; D010434:Pepsin A
enzymatic hydrolysis; soybean 2S protein; soybean Kunitz trypsin inhibitor; soybean allergy