A mesofluidic platform integrating on-chip probe ultrasonication for multiple sample pretreatment involving denaturation, reduction, and digestion in protein identification assays by mass spectrometry.
The integration of ultrasound (US)-assisted sample processing on-chip in a lab-on-a-valve (LOV) format for automated high-throughput shotgun proteomic assays is herein presented for the first time. The proof of concept of this system was demonstrated with the analysis of three proteins and sera from patients with lymphoma or myeloma.
Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.
J D
JD
Nunes-Miranda
Cristina
C
Núñez
Hugo M
HM
Santos
G
G
Vale
Miguel
M
Reboiro-Jato
Florentino
F
Fdez-Riverola
Carlos
C
Lodeiro
Manuel
M
Miró
J L
JL
Capelo
D014408:Biomarkers, Tumor / Q000032:analysis*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D046208:Microchip Analytical Procedures / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D011489:Protein Denaturation
D014408:Biomarkers, Tumor