Effects of grapefruit (Citrus paradisi MACF) (Rutaceae) peel oil against developmental stages of Aedes aegypti (Diptera: Culicidae).
Laboratory bioassay of the essential oil extracted from the grapefruit (Citrus paradisi) peel by steam distillation was carried out against the developmental stages of the yellow fever vector Aedes aegypti to evaluate its toxicity, and ovicidal and larvicidal potency. Volatile oil components isolated and characterized by coupled gas chromatography/mass spectrometry included varying levels of monoterpene aldehydes, alcohols, and esters. Test results of the essential oil showed that egg hatching was completely inhibited at 400 ppm, while further development of 1st to 2nd larval stage was inhibited at 100 ppm. Regression analysis results also indicated that the peel essential oil significantly (p<0.01) reduced the viability of the test eggs and inhibited the development of 1st larval stage to 2nd larval instar. The LC50 and LC90 values obtained for 2nd instars (180.460, 334.629 ppm, respectively); and for 4th instars (210.937, 349.489 ppm, respectively) after 24-hour exposure were time but not dose dependent, as each LC value was a product of an inverse relationship between the oil concentration and exposure time. The results indicated that the peel oil could be a potent persistent larvicide.
Department of Zoology and Environmental Biology, University of Nigeria, Nsukka, Enugu State.
Njoku
N
Ivoke
Department of Zoology and Environmental Biology, University of Nigeria, Nsukka, Enugu State.
Priscilla C
PC
Ogbonna
Department of Zoology and Environmental Biology, University of Nigeria, Nsukka, Enugu State.
Felicia N
FN
Ekeh
Department of Zoology and Environmental Biology, University of Nigeria, Nsukka, Enugu State.
Ngozi E
NE
Ezenwaji
Department of Zoology and Environmental Biology, University of Nigeria, Nsukka, Enugu State.
Chinedu I
CI
Atama
Department of Zoology and Environmental Biology, University of Nigeria, Nsukka, Enugu State.
Vincent C
VC
Ejere
Department of Home Science, Nutrition and Dietetics, University of Nigeria, Nsukka, Enugu State.
Uwakwe S
US
Onoja
Department of Zoology and Environmental Biology, University of Nigeria, Nsukka, Enugu State.
Joseph E
JE
Eyo
D000330:Aedes / Q000187:drug effects*; D000818:Animals; D032083:Citrus paradisi; D004305:Dose-Response Relationship, Drug; D005260:Female; D007306:Insecticides / Q000737:chemistry / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects; D007928:Lethal Dose 50; D008297:Male; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology*; D010063:Ovum / Q000187:drug effects
D007306:Insecticides; D009822:Oils, Volatile