Shotgun proteomic analysis of porcine colostrum and mature milk.
The epitheliochorial nature of the porcine placenta prevents the transfer of maternal immunity. Therefore, ingestion of the colostrum immediately after birth is crucial for neonatal piglets to acquire passive immunity from the sow. We performed a shotgun proteomic analysis of porcine milk to reveal in detail the protein composition of porcine milk. On the basis of the Swiss-Prot database, 113 and 118 proteins were identified in the porcine colostrum and mature milk, respectively, and 50 of these proteins were common to both samples. Some immune-related proteins, including interleukin-18 (IL-18), were unique to the colostrum. The IL-18 concentration in the colostrum and mature milk of four sows was measured to validate the proteomic analysis, and IL-18 was only detected in the colostrum (191.0 ± 53.9 pg/mL) and not in mature milk. In addition, some proteins involved in primary defense, such as azurocidin, which has never been detected in any other mammal's milk, were also identified in the colostrum.
Laboratory of Animal Science, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Ujitawara, Japan.
Shohei
S
Ogawa
Takamitsu
T
Tsukahara
Ryoichiro
R
Nishibayashi
Masako
M
Nakatani
Mie
M
Okutani
Nobuo
N
Nakanishi
Kazunari
K
Ushida
Ryo
R
Inoue
D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000032:analysis / Q000302:isolation & purification; D001798:Blood Proteins / Q000032:analysis / Q000302:isolation & purification; D002352:Carrier Proteins / Q000032:analysis / Q000302:isolation & purification; D002853:Chromatography, Liquid; D003126:Colostrum / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020382:Interleukin-18 / Q000032:analysis / Q000302:isolation & purification; D007774:Lactation / Q000378:metabolism; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000032:analysis* / Q000302:isolation & purification*; D036801:Parturition / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods*; D013552:Swine / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
C062991:AZU1 protein, human; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D002352:Carrier Proteins; D020382:Interleukin-18; D008894:Milk Proteins; D020543:Proteome
LC-MS/MS; colostrum; mature milk; proteome; sow