[Comparative analysis of conservation and regulatory network on core transcription factors in mouse inner ear development].
During vertebrate inner ear development, several core genes, such as Six1, Six4, Pax2, Pax8, Foxi1, Dlx5, Gbx2, Irx2/3, and Msx1, are crucial to the regulation of the otic placode induction. In order to get the gene regulatory network during inner ear development, bioinformatics methods were adopted to analyze conservation and regulation of the core transcription factors in mice. Pax2, Pax8, Foxi1, and Dlx5 remained to be the main regulators during inner ear development, which was consistent with the gene regulatory network from literature. Six1 was regulated by many transcription factors, and Gbx2, Irx2/3, and Msx1 played important roles in the regulatory network. The differences in the constructed regulatory network were reasonably analyzed. It was predicted that Msxl regulated the expression of Six1 and Gbx2. In addition, several transcription factors, such as Sox5, Lhx2, Rax, Otx1, Otx2, Pitxl, Pitx2, Nkx2-5, Irx4, Irx6, Dlx2, Hmx1/2/3, Pou4f3, Pax4 and Tlx2, were found to be involved in the regulatory network. Our results provide an improved understanding of the regulatory mechanism during inner ear development.
The First Clinical Medical College, Nanjing Medical University, Nanjing 210029, China. erwin_0108@sina.com
Zhi-Qiang
ZQ
Chen
School of Basic Medicine, Nanjing Medical University, Nanjing 210029, China.
Xin-Huan
XH
Han
School of Basic Medicine, Nanjing Medical University, Nanjing 210029, China.
Qin-Jun
QJ
Wei
The First Clinical Medical College, Nanjing Medical University, Nanjing 210029, China.
Guang-Qian
GQ
Xing
School of Basic Medicine, Nanjing Medical University, Nanjing 210029, China.
Xin
X
Cao
D000595:Amino Acid Sequence; D000818:Animals; D007758:Ear, Inner / Q000254:growth & development / Q000378:metabolism*; D053263:Gene Regulatory Networks; D006801:Humans; D051379:Mice / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D008969:Molecular Sequence Data; D051381:Rats; D016415:Sequence Alignment; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D014157:Transcription Factors