Molecular Diagnosis of Helicobacter Pylori Strain by 16S rDNA PCR Amplification and Direct Sequencing.
OBJECTIVE
Rapid detection of H.pylori strains by PCR-Sequencing.


METHODS
16S rDNA amplification by PCR from template genomic DNA, confirmation of amplicon size by agarose gel electrophoresis, sequencing of amplicons by automated sequencer, analysis of sequences by NCBI -BLAST software.


RESULTS
The PCR -Sequencing and analysis of the sequence data by BLAST resulted in detection of the strain to be of H.pylori strain#26695.


CONCLUSIONS
The pathogenicity of H.pylori depends on the strain of the bacteria, PCR-Sequencing and analysis of the sequence data by BLAST can be a very quick and useful diagnostic method of the pathogen.
Department of Biological Sciences, Elizabethcity State University under, The University of North Carolina, Elizabethcity, NC-27909, USA.
Hirendra Nath
HN
Banerjee
Department of Biological Sciences, Elizabethcity State University under, The University of North Carolina, Elizabethcity, NC-27909, USA.
Monique
M
Gramby
Department of Biological Sciences, Elizabethcity State University under, The University of North Carolina, Elizabethcity, NC-27909, USA.
Zack
Z
Hawkins
BLAST; DNA sequencing; H.pylori; Pcr