Genome-wide gene expression profiles in lung tissues of pig breeds differing in resistance to porcine reproductive and respiratory syndrome virus.
Porcine reproductive and respiratory syndrome (PRRS) caused by PRRS virus (PRRSV) is an infectious disease characterized by severe reproductive deficiency in pregnant sows, typical respiratory symptoms in piglets, and high mortality rate of piglets. In this study, we employed an Affymetrix microarray chip to compare the gene expression profiles of lung tissue samples from Dapulian (DPL) pigs (a Chinese indigenous pig breed) and Duroc×Landrace×Yorkshire (DLY) pigs after infection with PRRSV. During infection with PRRSV, the DLY pigs exhibited a range of clinical features that typify the disease, whereas the DPL pigs showed only mild signs of the disease. Overall, the DPL group had a lower percentage of CD4(+) cells and lower CD4(+)/CD8(+)ratios than the DLY group (p<0.05). For both IL-10 and TNF-α, the DLY pigs had significantly higher levels than the DPL pigs (p<0.01). The DLY pigs have lower serum IFN-γ levels than the DPL pigs (p<0.01). The serum IgG levels increased slightly from 0 dpi to 7 dpi, and peaked at 14 dpi (p<0.0001). Microarray data analysis revealed 16 differentially expressed (DE) genes in the lung tissue samples from the DLY and DPL pigs (q≤5%), of which LOC100516029 and LOC100523005 were up-regulated in the PRRSV-infected DPL pigs, while the other 14 genes were down-regulated in the PRRSV-infected DPL pigs compared with the PRRSV-infected DLY pigs. The mRNA expression levels of 10 out of the 16 DE genes were validated by real-time quantitative RT-PCR and their fold change was consistent with the result of microarray data analysis. We further analyzed the mRNA expression level of 8 differentially expressed genes between the DPL and DLY pigs for both uninfected and infected groups, and found that TF and USP18 genes were important in underlying porcine resistance or susceptibility to PRRSV.
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong Province, The People's Republic of China ; College of Life Science, Linyi University, Linyi, Shandong Province, The People's Republic of China.
Jinyi
J
Xing
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong Province, The People's Republic of China ; College of Animal Science, Tarim University, Ala'er, Xinjiang Uygur Autonomous Region, The People's Republic of China.
Feng
F
Xing
Department of Mathematics, The University of Southern Mississippi, Hattiesburg, Mississippi, United States of America.
Chenhua
C
Zhang
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong Province, The People's Republic of China ; College of Life Science, Linyi University, Linyi, Shandong Province, The People's Republic of China.
Yujie
Y
Zhang
School of Computing, The University of Southern Mississippi, Hattiesburg, Mississippi, United States of America.
Nan
N
Wang
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong Province, The People's Republic of China.
Yanping
Y
Li
School of Computing, The University of Southern Mississippi, Hattiesburg, Mississippi, United States of America.
Lijuan
L
Yang
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong Province, The People's Republic of China.
Chenglan
C
Jiang
School of Computing, The University of Southern Mississippi, Hattiesburg, Mississippi, United States of America.
Chaoyang
C
Zhang
School of Computing, The University of Southern Mississippi, Hattiesburg, Mississippi, United States of America.
Changhong
C
Wen
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong Province, The People's Republic of China.
Yunliang
Y
Jiang
D000818:Animals; D016000:Cluster Analysis; D060467:Disease Resistance / Q000235:genetics; D063990:Gene Ontology; D016678:Genome; D008168:Lung / Q000378:metabolism* / Q000821:virology; D020411:Oligonucleotide Array Sequence Analysis; D019318:Porcine Reproductive and Respiratory Syndrome / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity; D034421:Sus scrofa / Q000235:genetics / Q000276:immunology* / Q000821:virology; D013552:Swine / Q000235:genetics / Q000276:immunology / Q000821:virology; D059467:Transcriptome
D012333:RNA, Messenger