High fidelity nanopatterning of proteins onto well-defined surfaces through subtractive contact printing.
In the pursuit to develop enhanced technologies for cellular bioassays as well as understand single cell interactions with its underlying substrate, the field of biotechnology has extensively utilized lithographic techniques to spatially pattern proteins onto surfaces in user-defined geometries. Microcontact printing (μCP) remains an incredibly useful patterning method due to its inexpensive nature, scalability, and the lack of considerable use of specialized clean room equipment. However, as new technologies emerge that necessitate various nano-sized areas of deposited proteins, traditional μCP methods may not be able to supply users with the needed resolution size. Recently, our group developed a modified "subtractive μCP" method which still retains many of the benefits offered by conventional μCP. Using this technique, we have been able to reach resolution sizes of fibronectin as small as 250 nm in largely spaced arrays for cell culture. In this communication, we present a detailed description of our subtractive μCP procedure that expands on many of the little tips and tricks that together make this procedure an easy and effective method for controlling protein patterning.
Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta Georgia, USA; Petit Institute for Bioengineering and Bioscience, Atlanta, Georgia, USA.
José R
JR
García
Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta Georgia, USA; Petit Institute for Bioengineering and Bioscience, Atlanta, Georgia, USA.
Ankur
A
Singh
Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta Georgia, USA; Petit Institute for Bioengineering and Bioscience, Atlanta, Georgia, USA.
Andrés J
AJ
García
D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D005353:Fibronectins / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D011327:Printing / Q000379:methods*; D013499:Surface Properties
D005353:Fibronectins
Cell adhesion; Fibronectin; Micropatterning; Self-assembled monolayers (SAMs); Soft lithography