Fluoride-induced apoptosis and expressions of caspase proteins in the kidney of carp (Cyprinus carpio).
The study was conducted to investigate oxidative stress, apoptosis, and protein expressions of caspase-3, 8, and 9 in kidney of the carp juveniles exposed to 0, 40, 80, 120, and 160 mg L(-1) of fluoride (in the form of NaF) for 90 days. The results showed that dose- and time-dependent decrease of SOD and GSH and dose- and time-dependent increase of MDA were observed in the carp juveniles, which suggested that fluoride induced oxidative damage accompanied with morphological changes and significant apoptosis in fish exposed to fluoride, especially in the higher doses. Fluoride exposure also significantly elevated the protein expressions of caspase-3, 8, and 9. In conclusion, these results indicate that chronic exposure to fluoride causes oxidative stress, damages the kidney structure, and results in renal apoptosis by caspase-dependent pathway.
State Key Laboratory of Ecological Animal Husbandry and Environmental Veterinary Medicine, College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, China.
Jianjie
J
Chen
State Key Laboratory of Ecological Animal Husbandry and Environmental Veterinary Medicine, College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, China.
Jinling
J
Cao
State Key Laboratory of Ecological Animal Husbandry and Environmental Veterinary Medicine, College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, China.
Jundong
J
Wang
State Key Laboratory of Ecological Animal Husbandry and Environmental Veterinary Medicine, College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, China.
Ruhui
R
Jia
State Key Laboratory of Ecological Animal Husbandry and Environmental Veterinary Medicine, College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, China.
Wenjuan
W
Xue
Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, Liaoning, 110016, China.
Lingtian
L
Xie
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002347:Carps / Q000254:growth & development / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D020169:Caspases / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D012969:Sodium Fluoride / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism
D012969:Sodium Fluoride; D013482:Superoxide Dismutase; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9; D020169:Caspases; D005978:Glutathione
Cyprinus carpio; apoptosis; expression of caspase proteins; fluoride; kidney; oxidative stress