Comparative pharmacokinetics of a marker compound, baicalin in KOB extract after oral administration to normal and allergic-induced rats.
KOB extracts are a polyherbal medicine had been prescribed for the treatment of hyperhydrosis and allergic diseases such as allergic asthma and rhinitis in oriental clinics. Therefore, the pharmacokinetic studies of the KOB extract administered orally to normal rats and rhinitis-induced rats to understand the correlation of the efficacy and plasma concentration of KOB in patients of allergic rhinitis in future were performed. The study was conducted according to administration for pure baicalin in normal rats, baicalin in KOB extract in normal rats and rhinitis-induced rats. Baicalin in rat plasma was analyzed and validated by HPLC analysis. The interday precision based on the standard deviation of replicates of quality control samples ranged from 3.6% to 7.9% with accuracy ranging from 92.9% to 101.2% for baicalin. Based on validated analysis, pharmacokinetic study was carried out. Pure baicalin in normal rats and baicalin in KOB extract in normal rats showed bimodal curves due to direct absorption and glucuronidation. The Tmax, Cmax and AUC of pure baicalin in normal rats or baicalin in KOB extract in normal rats were 12 h, 0.68 µg/ml and 9.85 µg h/ml, respectively, or 12 h, 0.46 µg/ml and 6.36 µg h/ml, respectively. The analytical method showed excellent sensitivity, precision and accuracy, being successfully employed in a pharmacokinetic study of polyherbal medicine, KOB extract. Allergic-induced condition did not affect the pharmacokinetics of KOB extracts, suggesting KOB extracts did not require dosage adjustment in subjects with allergic-induced diseases.
College of Pharmacy and Institute of Drug Research and Development, Chungnam National University , Daejeon , South Korea and.
Jong-Suep
JS
Baek
Chan-Ju
CJ
Hwang
Hyo-Won
HW
Jung
Yong-Ki
YK
Park
Young-Ho
YH
Kim
Jong-Seong
JS
Kang
Cheong-Weon
CW
Cho
D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005419:Flavonoids / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics*; D007408:Intestinal Absorption / Q000502:physiology; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics*; D028321:Plant Preparations / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012220:Rhinitis / Q000097:blood / Q000188:drug therapy*
D005419:Flavonoids; D010936:Plant Extracts; D028321:Plant Preparations; C038044:baicalin
Allergic-induced rat; KOB extract; baicalin; pharmacokinetic; polyherbal