Wide-scope analysis of pesticide and veterinary drug residues in meat matrices by high resolution MS: detection and identification using Exactive-Orbitrap.
A multiresidue and multiclass method for the simultaneous determination of more than 350 compounds including pesticides, biopesticides and veterinary drugs in different meat matrices (beef, pork and chicken) by ultra-high performance liquid chromatography coupled to Orbitrap MS has been developed. In the present study, the determination of fragments was accomplished as an essential tool for a reliable identification of compounds using high resolution MS. To obtain these fragments, different strategies have been carried out in order to ensure an appropriate fragment assignment and identification. The analytical method is suitable for qualitative analysis, and it was also evaluated for quantitative analysis. Generic extraction conditions were optimized, obtaining adequate recovery and precision values for most of the studied analytes (>290). The limits of detection ranged from 2 to 16 µg kg(-1). Limits of quantification were 10 µg kg(-1) with the exception of few compounds with a higher value (50 or 100 µg kg(-1)). Limits of identification were also established, and they ranged from 2 to 150 µg kg(-1). This method was applied to the analysis of 18 meat samples and some veterinary drugs as enrofloxacin and sulfadiazine were detected and further identified/quantified (with triple quadrupole) in two different samples at 33 µg kg(-1) and trace levels, respectively. No pesticides were detected in the analyzed samples.
Research Group 'Analytical Chemistry of Contaminants', Department of Chemistry and Physics, Research Centre for Agricultural and Food Biotechnology (BITAL), University of Almeria, Agrifood Campus of International Excellence, ceiA3, E-04120, Almeria, Spain.
María Luz
ML
Gómez-Pérez
Roberto
R
Romero-González
Patricia
P
Plaza-Bolaños
Eric
E
Génin
José Luis
JL
Martínez Vidal
Antonia
A
Garrido Frenich
D000818:Animals; D002417:Cattle; D002645:Chickens; D004350:Drug Residues / Q000032:analysis* / Q000737:chemistry; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D008460:Meat / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine; D019155:Veterinary Drugs / Q000032:analysis* / Q000737:chemistry
D010573:Pesticide Residues; D019155:Veterinary Drugs
fragment database; high resolution MS; meat; pesticide; veterinary drug