How does pollen chemistry impact development and feeding behaviour of polylectic bees?
Larvae and imagos of bees rely exclusively on floral rewards as a food source but host-plant range can vary greatly among bee species. While oligolectic species forage on pollen from a single family of host plants, polylectic bees, such as bumblebees, collect pollen from many families of plants. These polylectic species contend with interspecific variability in essential nutrients of their host-plants but we have only a limited understanding of the way in which chemicals and chemical combinations influence bee development and feeding behaviour. In this paper, we investigated five different pollen diets (Calluna vulgaris, Cistus sp., Cytisus scoparius, Salix caprea and Sorbus aucuparia) to determine how their chemical content affected bumblebee colony development and pollen/syrup collection. Three compounds were used to characterise pollen content: polypeptides, amino acids and sterols. Several parameters were used to determine the impact of diet on micro-colonies: (i) Number and weight of larvae (total and mean weight of larvae), (ii) weight of pollen collected, (iii) pollen efficacy (total weight of larvae divided by weight of the pollen collected) and (iv) syrup collection. Our results show that pollen collection is similar regardless of chemical variation in pollen diet while syrup collection is variable. Micro-colonies fed on S. aucuparia and C. scoparius pollen produced larger larvae (i.e. better mates and winter survivors) and fed less on nectar compared to the other diets. Pollen from both of these species contains 24-methylenecholesterol and high concentrations of polypeptides/total amino acids. This pollen nutritional "theme" seems therefore to promote worker reproduction in B. terrestris micro-colonies and could be linked to high fitness for queenright colonies. As workers are able to selectively forage on pollen of high chemical quality, plants may be evolutionarily selected for their pollen content, which might attract and increase the degree of fidelity of generalist pollinators, such as bumblebees.
Research Institute of Biosciences, Laboratory of Zoology, University of Mons, Mons, Belgium.
Maryse
M
Vanderplanck
Research Institute of Biosciences, Laboratory of Zoology, University of Mons, Mons, Belgium ; Evolutionary Biology and Ecology, Université Libre de Bruxelles, Brussels, Belgium.
Romain
R
Moerman
Research Institute of Biosciences, Laboratory of Zoology, University of Mons, Mons, Belgium.
Pierre
P
Rasmont
Unit of Analysis Quality and Risk, Laboratory of Analytical Chemistry, Gembloux Agro-Bio Tech, University of Liege, Gembloux, Belgium.
Georges
G
Lognay
Unit of Biological and Industrial Chemistry, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium.
Bernard
B
Wathelet
Research Institute of Biosciences, Department of Proteomic and Protein Biochemistry, University of Mons, Mons, Belgium.
Ruddy
R
Wattiez
Research Institute of Biosciences, Laboratory of Zoology, University of Mons, Mons, Belgium.
Denis
D
Michez
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001516:Bees / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology*; D002784:Cholesterol / Q000031:analogs & derivatives / Q000378:metabolism; D004032:Diet; D005247:Feeding Behavior / Q000502:physiology*; D007814:Larva / Q000254:growth & development / Q000378:metabolism / Q000502:physiology; D010455:Peptides / Q000378:metabolism; D011058:Pollen / Q000378:metabolism*; D054817:Pollination / Q000502:physiology
D000596:Amino Acids; D010455:Peptides; D002784:Cholesterol; C002537:24-methylenecholesterol