Highly sensitive and selective determination of pyrazinamide at poly-L-methionine/reduced graphene oxide modified electrode by differential pulse voltammetry in human blood plasma and urine samples.
In this current study we used electrochemically active film which contains poly-L-methionine (PMET) and electrochemically reduced graphene oxide (ERGO) on glassy carbon electrode (GCE) for pyrazinamide (PZM) detection. The electrocatalytic response of analyte at PMET/ERGO/GCE film was measured using both cyclic voltammetry (CV) and differential pulse voltammetry (DPV). In addition, electrochemical impedance studies revealed that the smaller R(ct) value observed at PMET/ERGO film modified GCE which authenticates its good conductivity and faster electron transfer rate. The prepared PMET/ERGO/GCE film exhibits excellent DPV response towards PZM and the reduction peak current increased linearly with respect to PZM concentration in the linear range between 0.4 μM to 1129 μM with a sensitivity of 0.266 μA μM(-1) cm(-2). Real sample studies were carried out in human blood plasma and urine samples, which offered good recovery and revealed the promising practicality of the sensor for PZM detection. The proposed sensor displayed a good selectivity, repeatability, sensitivity with appreciable consistency and good reproducibility. In addition, the proposed electrochemical sensor showed good results towards the commercial pharmaceutical PZM samples.
Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan, ROC.
Srikanth
S
Cheemalapati
Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan, ROC.
Balamurugan
B
Devadas
Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan, ROC. Electronic address: smchen78@ms15.hinet.net.
Shen-Ming
SM
Chen
D000328:Adult; D000995:Antitubercular Agents / Q000097:blood / Q000652:urine; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D004566:Electrodes; D004579:Electron Transport; D006108:Graphite / Q000737:chemistry*; D064368:Healthy Volunteers; D006801:Humans; D010084:Oxidation-Reduction; D010087:Oxides; D010455:Peptides / Q000737:chemistry*; D011718:Pyrazinamide / Q000097:blood / Q000652:urine; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000995:Antitubercular Agents; D010087:Oxides; D010455:Peptides; C034757:polymethionine; D011718:Pyrazinamide; D006108:Graphite
Blood plasma; Differential pulse voltammetry; Electrochemically reduced graphene oxide; Methionine; Pyrazinamide; Reduction