Ameliorative effect of polyphenols from Padina boergesenii against ferric nitrilotriacetate induced renal oxidative damage: With inhibition of oxidative hemolysis and in vitro free radicals.
The aim of this study was to evaluate the antioxidant activities of diethyl ether (DEE) and methanol (M) extracts from brown alga Padina boergesenii using in vitro and in vivo antioxidant assay, which may help to relate the antioxidant properties with the possible outline of its ameliorative effect. M extract showed higher radical scavenging activity through ferric reducing antioxidant power 139.11 µmol tannic acid equivalent/g; DPPH 71.32 ± 0.56%; deoxyribose radical 88.31 ± 0.47%, and total antioxidant activity 0.47 ± 0.02 mg ascorbic acid equivalents/g. Oxidative red blood cell (RBC) hemolysis inhibition rate was significantly higher in M extract (150 mg/kg body weight) in reference to total phenolic content (r = 0.935). Rats administered with DEE and M extracts (150 mg/kg body weight) for seven days before the administration of ferric nitrilotriacetate (9 mg of Fe/mg/kg bodyweight). Rats pretreated with extracts significantly changed the level of renal microsomal lipid peroxidation, glutathione, and antioxidant enzymes in post-mitochondrial supernatant (P < 0.05). Ameliorative effect of extracts against renal oxidative damage was evident in rat kidney through changes in necrotic and epithelial cells. HPTLC technique has identified the presence of rutin with reference to retardation factor (Rf ) in both the extracts. These findings support the source of polyphenols (rutin) from P. boergesenii had potent antioxidant activity; further work on isolation of bioactive compounds can be channeled to develop as a natural antioxidant.
Department of Marine Sciences, Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai, 608 502, Tamil Nadu, India.|Department of Pathology and Laboratory Medicine, UBC James Hogg Research Centre, St. Paul's Hospital, Vancouver-V6Z 1Y6, British Columbia, Canada.
Karthikeyan
K
Rajamani
Department of Medical Biochemistry, Rajah Muthiah Medical College, Annamalai University, Annamalainagar, 608 002, Tamil Nadu, India.
V C
VC
Renju
Department of Medical Biochemistry, Rajah Muthiah Medical College, Annamalai University, Annamalainagar, 608 002, Tamil Nadu, India.
S
S
Sethupathy
Department of Marine Sciences, Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai, 608 502, Tamil Nadu, India.
Somasundaram S
SS
Thirugnanasambandan
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D003855:Deoxyribose / Q000737:chemistry / Q000494:pharmacology; D004847:Epithelial Cells / Q000187:drug effects; D005260:Female; D005290:Ferric Compounds / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D005609:Free Radicals / Q000633:toxicity*; D006461:Hemolysis / Q000187:drug effects*; D066298:In Vitro Techniques; D007674:Kidney Diseases / Q000139:chemically induced* / Q000378:metabolism / Q000517:prevention & control*; D008861:Microsomes / Q000187:drug effects; D009336:Necrosis; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects*; D000459:Phaeophyceae / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012431:Rutin / Q000032:analysis; D013634:Tannins / Q000494:pharmacology
D000975:Antioxidants; D005290:Ferric Compounds; D005609:Free Radicals; D010936:Plant Extracts; D059808:Polyphenols; D013634:Tannins; D003855:Deoxyribose; D012431:Rutin; D009571:Nitrilotriacetic Acid; C020326:ferric nitrilotriacetate
Padina boergesenii; RBC hemolysis; antioxidant; ferric nitrilotriacetate; polyphenols; renal oxidative damage