The apoptotic pathways effect of fine particulate from cooking oil fumes in primary fetal alveolar type II epithelial cells.
Apoptosis occurs along three major pathways: (i) an extrinsic pathway, mediated by death receptors; (ii) an intrinsic pathway centered on mitochondria; and (iii) an ER-stress pathway. We investigated the apoptotic pathway effects of cooking oil fumes (COF) in fetal lung type II-like epithelium cells (AEC II). Exposure to COF caused up-regulation of the pro-apoptotic protein Bax and down-regulation of the anti-apoptotic protein Bcl-2. COF induced the mitochondrial permeability transition, an early event in apoptosis; cytochrome c was translocated from the mitochondria to the cytoplasm and nucleus. Caspase-9 and caspase-3 were activated, as a consequence of the mitochondrial permeability transition. The death receptor apoptotic pathway was triggered by COF, as indicated by a change in Fas expression, resulting in increased caspase-8 content. COF exposure arrested the cell cycle the at G0-G1 phase. In summary, COF can lead to apoptosis via mitochondrial and death receptor pathways in AEC II cells.
School of Public Health, Anhui Medical University, Hefei, China.
Zhen
Z
Che
School of Public Health, Anhui Medical University, Hefei, China.
Ying
Y
Liu
School of Public Health, Anhui Medical University, Hefei, China.
Yanyan
Y
Chen
School of Public Health, Anhui Medical University, Hefei, China. Electronic address: qshq@163.com.
Jiyu
J
Cao
School of Public Health, Anhui Medical University, Hefei, China.
Chunmei
C
Liang
School of Public Health, Anhui Medical University, Hefei, China.
Lei
L
Wang
School of Public Health, Anhui Medical University, Hefei, China.
Rui
R
Ding
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D003296:Cooking; D004042:Dietary Fats, Unsaturated; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D005260:Female; D005333:Fetus / Q000166:cytology / Q000187:drug effects; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR; D010316:Particle Size; D052638:Particulate Matter / Q000633:toxicity*; D061251:Primary Cell Culture; D011650:Pulmonary Alveoli / Q000166:cytology / Q000187:drug effects* / Q000196:embryology; D020545:Respiratory Mucosa / Q000166:cytology / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects; D012906:Smoke / Q000009:adverse effects*
D004042:Dietary Fats, Unsaturated; D052638:Particulate Matter; D012906:Smoke
AEC II; Apoptosis; COF; Caspase; Cell cycles; Flow cytometry