Assessment of cytotoxicity, fetotoxicity, and teratogenicity of Plathymenia reticulata Benth Barks aqueous extract.
Scientific assessment of harmful interactions of chemicals over the entire reproductive cycle are divided into three segments based on the period: from premating and mating to implantation (I), from implantation to major organogenesis (II), and late pregnancy and postnatal development (III). We combined the segments I and II to assess Plathymenia reticulata aqueous extract safety. In order to investigate reproductive toxicity (segment I), pregnant rats received orally 0.5 or 1.0 g/kg of extract, daily, during 18 days. These concentrations were determined by a preliminary in vitro LD50 test in CHO-k1 cells. A control group received deionized water. The offspring was removed at the 19th day, by caesarean, and a teratology study (segment II) was carried out. The corpora lutea, implants, resorptions, live, and dead fetuses were then counted. Placenta and fetuses were weighted. External and visceral morphology were provided by the fixation of fetuses in Bouin, whereas skeletal analysis was carried out on the diaphanizated ones. The increase in the weights of placenta and fetuses was the only abnormality observed. Since there was no sign of alteration on reproduction parameters at our experimental conditions, we conclude that P. reticulata aqueous extract is safe at 0.5 to 1.0 g/kg and is not considered teratogenic.
Post-Graduate Program in Pharmaceutical Sciences, University of Sorocaba, UNISO, Rodovia Raposo Tavares, km 92.5, 18023-000 Sorocaba, SP, Brazil.
Lia
L
de Barros Leite Albuquerque
Federal University of Pampa, CIPBiotec, UNIPAMPA, Avenida Antonio Trilha 1847, 97300-000 São Gabriel, RS, Brazil.
Cháriston André
CA
Dal Belo
Post-Graduate Course in Environmental Sciences, PGCiamb, Federal University of Tocantins, UFT, Avenida NS 15 ALC NO 14, 109 Norte, 77001-090 Tocantins, Brazil.
Marcio Galdino
MG
dos Santos
Federal University of São Paulo, UNIFESP, R. Prof. Artur Riedel 275, 09972-270 Diadema, SP, Brazil.
Patricia Santos
PS
Lopes
Post-Graduate Program in Pharmaceutical Sciences, University of Sorocaba, UNISO, Rodovia Raposo Tavares, km 92.5, 18023-000 Sorocaba, SP, Brazil.
Marli
M
Gerenutti
Post-Graduate Program in Pharmaceutical Sciences, University of Sorocaba, UNISO, Rodovia Raposo Tavares, km 92.5, 18023-000 Sorocaba, SP, Brazil.
Yoko
Y
Oshima-Franco
D000818:Animals; D004305:Dose-Response Relationship, Drug; D007887:Fabaceae / Q000737:chemistry; D005260:Female; D005333:Fetus / Q000187:drug effects*; D006801:Humans; D038081:Organogenesis / Q000187:drug effects*; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D011247:Pregnancy; D051381:Rats; D013723:Teratogens / Q000737:chemistry / Q000494:pharmacology; D014867:Water / Q000737:chemistry
D010936:Plant Extracts; D013723:Teratogens; D014867:Water