Serum prolidase enzyme activity and oxidative stress levels in patients with acute hemorrhagic stroke.
BACKGROUND
It has been indicated that oxidative damage contributes to secondary brain injury in both ischemic and hemorrhagic stroke patients. Collagen is a major component of the extracellular matrix, and prolidase plays a role in collagen synthesis. The aim of this study was to evaluate the serum prolidase activity, nitric oxide (NO) levels, total antioxidant capacity (TAC) and total oxidant status (TOS) in patients with acute hemorrhagic stroke.


METHODS
Twenty-five patients with acute hemorrhagic stroke and 25 controls were enrolled. Serum prolidase activity, catalase activity, NO levels, TAC and TOS were measured spectrophotometrically. Oxidative stress index (OSI) was calculated.


RESULTS
Serum TAC levels and catalase activity were significantly lower in acute hemorrhagic stroke patients than controls (both, p < 0.001), while NO levels, TOS levels, OSI values and prolidase activity were significantly higher (all, p < 0.01). When patients with acute hemorrhagic stroke were divided according to gender, no differences were observed between females and males in respect to serum prolidase enzyme activity, NO levels, TAC levels, TOS levels and OSI values (all, p > 0.05).


CONCLUSIONS
Findings from the study suggest an association between increased oxidative stress levels, decreased antioxidant levels and increased prolidase enzyme activity in patients with acute hemorrhagic stroke compared with controls. More studies are needed to elucidate mechanistic pathways on oxidative stress in patients with acute hemorrhagic stroke.
Department of Emergency Medicine, Yuzuncu Yıl University , Van , Turkey.
Hayriye
H
Gonullu
Mehmet
M
Aslan
Sevdegul
S
Karadas
Celal
C
Kati
Latif
L
Duran
Aysel
A
Milanlioglu
Mehmet Nuri
MN
Aydin
Halit
H
Demir
D000368:Aged; D016022:Case-Control Studies; D002374:Catalase / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D004150:Dipeptidases / Q000097:blood; D005260:Female; D006801:Humans; D020300:Intracranial Hemorrhages / Q000097:blood* / Q000150:complications / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000097:blood; D018384:Oxidative Stress; D020521:Stroke / Q000097:blood* / Q000150:complications / Q000503:physiopathology; D014280:Triglycerides / Q000097:blood
D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D014280:Triglycerides; D009569:Nitric Oxide; D002374:Catalase; D004150:Dipeptidases; C022449:proline dipeptidase