Investigation of some enzymatic activities contributing to the biosynthesis of galactolipid precursors in Vicia faba.
Assay conditions, intracellular distribution and change of activity during greening of etiolated seedlings of Vicia faba L. have been investigated for aldose reductase (EC 1.1.1.21), UDP-glucose pyrophosphorylase (EC 2.7.7.9), UDP-glucose 4-epimerase (EC 5.1.3.2) and phosphatidate phosphatase (EC 3.1.3.4). The first three enzymes were found to be soluble proteins of the cytoplasm, whereas phosphatidate phosphatase was found in the soluble and a sedimentable (15000 Xg) fraction.
Botanisches Institut, Universität Köln, Gyrhofstraße 15, D-5000, Köln 41, Federal Republic of Germany.
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Königs
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Heinz