LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts.
The ability of LC-NMR to detect simultaneously free and conjugated phytosterols in natural extracts was tested. The advantages and disadvantages of a gradient HPLC-NMR method were compared to the fast composition screening using SEC-NMR method. Fractions of free and conjugated phytosterols were isolated and analyzed by isocratic HPLC-NMR methods. The results of qualitative and quantitative analyses were in a good agreement with the literature data.
Institute of Chemical Process Fundamentals of the ASCR, v.v.i., Rozvojová 2/135, 16502 Prague 6, Czech Republic.
Stěpán
S
Horník
Institute of Chemical Process Fundamentals of the ASCR, v.v.i., Rozvojová 2/135, 16502 Prague 6, Czech Republic.
Marie
M
Sajfrtová
Institute of Chemical Process Fundamentals of the ASCR, v.v.i., Rozvojová 2/135, 16502 Prague 6, Czech Republic.
Jindřich
J
Karban
Institute of Chemical Process Fundamentals of the ASCR, v.v.i., Rozvojová 2/135, 16502 Prague 6, Czech Republic.
Jan
J
Sýkora
Institute of Organic Chemistry and Biochemistry of the ASCR, v.v.i., Flemingovo Náměstí 2, 16610 Prague 6, Czech Republic.
Anna
A
Březinová
Institute of Experimental Botany of the ASCR, v.v.i., Isotope Laboratory, Vídeňská 1083, 14220 Prague 4, Czech Republic ; Institute of Chemical Technology Prague, Faculty of Food and Biochemical Technology, Department of Chemistry of Natural Compounds, Technická 5, 16628 Prague 6, Czech Republic.
Zdeněk
Z
Wimmer