High specific monoclonal antibody production and development of an ELISA method for monitoring T-2 toxin in rice.
This research produced a highly-specific and sensitive anti-T-2 toxin monoclonal antibody (mAb), and developed a rapid and sensitive competitive indirect enzyme-linked immunosorbent assay (ELISA) method for monitoring T-2 toxin in rice. The mAb showed a negligible cross-reactivity value (CR) to most of the mycotoxins, and it could specifically bind to T-2 toxin without other mycotoxins, including HT-2 toxin (CR value at 3.08%), which exhibited a similar structure to T-2 toxin. The limit of detection (LOD) value, measured by IC10, was 5.80 μg/kg. In spiked samples, mean recoveries ranged from 72.0% to 108.5% with intraday and interday variation less than 16.8 and 13.7%. This proposed protocol was significantly confirmed by a reliable ultrahigh performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method and significant correlation was obtained.
College of Life Science, Yantai University , Yantai 264005, People's Republic of China.
Yanshen
Y
Li
Xiangshu
X
Luo
Shupeng
S
Yang
Xingyuan
X
Cao
Zhanhui
Z
Wang
Weimin
W
Shi
Suxia
S
Zhang
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D005506:Food Contamination / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D012275:Oryza / Q000737:chemistry*; D013605:T-2 Toxin / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology
D000911:Antibodies, Monoclonal; D013605:T-2 Toxin; C012351:HT-2 toxin