Preparation and evaluation of periodontal films based on polyelectrolyte complex formation.
Local intra-pocket drug delivery devices can provide an effective concentration of the antimicrobial agent at the site of action with avoidance of undesirable side effects. This study explored the application of chitosan-alginate and chitosan-pectin polyelectrolyte complex (PEC) films as drug release regulators for tetracycline HCl (Tc) to treat periodontal pockets. Periodontal films with 1:1 Tc:PEC ratio were prepared using 1:1 chitosan (Ch) to sodium alginate (A) or 1:3 Ch to pectin (P). The scanning electron microscope showed acceptable film appearance and differential scanning calorimetry analysis confirmed complex formation. The in vitro release studies for both films showed a burst drug release, followed by prolonged release for 70 h. A prolonged antibacterial activity of both films against Staphylococcus aureus ATCC 6538 was observed over a period of 21 days. Aging studies indicated that the five months storage period in freezer did not significantly influence the drug release profile or the antibacterial activity of both films. Clinical evaluation showed a significant reduction in pocket depth (p < 0.0001) to their normal values (≤3 mm). PEC films could be exploited as a prolonged drug release devices for treatment of periodontal pockets.
Department of Pharmaceutics, Faculty of Pharmacy and Drug Manufacturing, Pharos University in Alexandria , Alexandria , Egypt .
Abeer Ahmed
AA
Kassem
Fatma Ahmed
FA
Ismail
Viviane Fahim
VF
Naggar
Elsayed
E
Aboulmagd
D000464:Alginates / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology; D002152:Calorimetry, Differential Scanning; D002626:Chemistry, Pharmaceutical / Q000379:methods; D048271:Chitosan / Q000737:chemistry; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D065546:Drug Liberation; D004355:Drug Stability; D004356:Drug Storage; D004573:Electrolytes / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D010368:Pectins / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects; D013752:Tetracycline / Q000008:administration & dosage* / Q000494:pharmacology; D013997:Time Factors
D000464:Alginates; D000900:Anti-Bacterial Agents; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004573:Electrolytes; D006603:Hexuronic Acids; D010368:Pectins; D020723:Glucuronic Acid; D048271:Chitosan; D013752:Tetracycline
Chitosan-alginate; chitosan-pectin; clinical studies; release behavior; tetracycline HCl