A gold@silica core-shell nanoparticle-based surface-enhanced Raman scattering biosensor for label-free glucose detection.
The gold nanostar@silica core-shell nanoparticles conjugated with glucose oxidase (GOx) enzyme molecules have been developed as the surface-enhanced Raman scattering (SERS) biosensor for label-free detection of glucose. The surface-immobilized GOx enzyme catalyzes the oxidation of glucose, producing hydrogen peroxide. Under laser excitation, the produced H2O2 molecules near the Au nanostar@silica nanoparticles generate a strong SERS signal, which is used to measure the glucose concentration. The SERS signal of nanostar@silica∼GOx nanoparticle-based sensing assay shows the dynamic response to the glucose concentration range from 25 μM to 25 mM in the aqueous solution with the limit of detection of 16 μM. The sensing assay does not show any interference when glucose co-exists with both ascorbic acid and uric acid. The sensor can be applied to a saliva sample.
Department of Biotechnology, College of Science, University of Baghdad, Baghdad, Iraq; Department of Mechanical and Aerospace Engineering, West Virginia University, Morgantown, WV 26506-6106, USA. Electronic address: israaalizaidan@yahoo.com.
Israa
I
Al-Ogaidi
Department of Mechanical and Aerospace Engineering, West Virginia University, Morgantown, WV 26506-6106, USA.
Honglei
H
Gou
Department of Biotechnology, College of Science, University of Baghdad, Baghdad, Iraq.
Abdul Kareem A
AK
Al-Kazaz
Zystein, LLC, Arkansas, Fayetteville, USA.
Zoraida P
ZP
Aguilar
Department of Biotechnology, College of Science, University of Baghdad, Baghdad, Iraq.
Alice K
AK
Melconian
Department of Mechanical and Aerospace Engineering, West Virginia University, Morgantown, WV 26506-6106, USA.
Peng
P
Zheng
Department of Mechanical and Aerospace Engineering, West Virginia University, Morgantown, WV 26506-6106, USA.
Nianqiang
N
Wu
D001205:Ascorbic Acid / Q000737:chemistry; D015374:Biosensing Techniques; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005942:Gluconates / Q000032:analysis; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis; D053768:Metal Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction; D012822:Silicon Dioxide / Q000737:chemistry*; D013059:Spectrum Analysis, Raman; D014527:Uric Acid / Q000737:chemistry
D004800:Enzymes, Immobilized; D005942:Gluconates; D014527:Uric Acid; D006046:Gold; D012822:Silicon Dioxide; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose; D001205:Ascorbic Acid; C030691:gluconic acid
Biosensor; Glucose; Surface-enhanced Raman scattering (SERS)