Crystal structure determination of anti-DNA Fab A52.
A52 is a murine monoclonal antibody isolated from autoimmune New Zealand Black/New Zealand White F1 mice that recognizes single and double stranded DNA. This mouse strain spontaneously develops systemic lupus erythematosus-like symptoms and has served as a model for that disease for many years. The 1.62 Å crystal structure of the A52 Fab fragment reveals an H3 complementarity determining region with four closely spaced arginine residues, creating a positively charged surface to accommodate bound DNA.
The Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, 92037.
Robyn L
RL
Stanfield
Dan
D
Eilat
D000595:Amino Acid Sequence; D000818:Animals; D000974:Antibodies, Antinuclear / Q000737:chemistry* / Q000276:immunology*; D022801:Complementarity Determining Regions / Q000737:chemistry / Q000276:immunology; D004247:DNA / Q000276:immunology*; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry* / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016415:Sequence Alignment
D000974:Antibodies, Antinuclear; D022801:Complementarity Determining Regions; D007140:Immunoglobulin Fab Fragments; D004247:DNA
DNA; Fab; antibody; lupus erythematosus; systemic