Site-specific distribution of CD68-positive microglial cells in the brains of human midterm fetuses: a topographical relationship with growing axons.
Using 5 fetuses of gestational age (GA) of 15-16 weeks and 4 of GA of 22-25 weeks, we examined site- and stage-dependent differences in CD68-positive microglial cell distribution in human fetal brains. CD68 positive cells were evident in the floor of the fourth ventricle and the pons and olive at 15-16 weeks, accumulating in and around the hippocampus at 22-25 weeks. At both stages, the accumulation of these cells was evident around the optic tract and the anterior limb of the internal capsule. When we compared CD68-positive cell distribution with the topographical anatomy of GAP43-positive developing axons, we found that positive axons were usually unaccompanied by CD68-positive cells, except in the transpontine corticofugal tract and the anterior limb of the internal capsule. Likewise, microglial cell distribution did not correspond with habenulointerpeduncular tract. Therefore, the distribution of CD68-positive cells during normal brain development may not reflect a supportive role of these microglia in axonogenesis of midterm human fetuses.
Department of Neurology, Jeonbuk Regional Cardiocerebrovascular Disease Center, Institute of Wonkwang Medical Science, Wonkwang University School of Medicine, Iksan 570-711, Republic of Korea.
Kwang Ho
KH
Cho
Department of Neurology, Jeonbuk Regional Cardiocerebrovascular Disease Center, Institute of Wonkwang Medical Science, Wonkwang University School of Medicine, Iksan 570-711, Republic of Korea.
Jin Sung
JS
Cheong
Department of Anatomy, Chonbuk National University School of Medicine, 634-18 Geumam-dong, Deokjin-gu, Jeonju 561-712, Republic of Korea.
Ji Hyun
JH
Kim
Department of Anatomy, Akita University School of Medicine, Akita 010-8502, Japan.
Hiroshi
H
Abe
Division of Internal Medicine, Iwamizawa Kojin-kai Hospital, Iwamizawa 068-0833, Japan.
Gen
G
Murakami
Department of Surgery, Chonbuk National University School of Medicine, 634-18 Geumam-dong, Deokjin-gu, Jeonju 561-712, Republic of Korea.
Baik Hwan
BH
Cho
D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism*; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology / Q000378:metabolism*; D001369:Axons / Q000378:metabolism; D001921:Brain / Q000254:growth & development* / Q000276:immunology; D005260:Female; D005333:Fetus / Q000196:embryology / Q000276:immunology*; D005865:Gestational Age; D006801:Humans; D017628:Microglia / Q000276:immunology / Q000378:metabolism; D011247:Pregnancy; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; C067980:CD68 antigen, human; D000097382:CD68 Molecule