Fecal microbiota imbalance in Mexican children with type 1 diabetes.
Dysbiosis of the intestinal microbiota affecting the gut barrier could be triggering Type 1 Diabetes (T1D), the second most frequent autoimmune disease in childhood. This study compared the structure of the fecal microbiota in 29 mestizo children aged 7-18 years, including 8 T1D at onset, 13 T1D after 2 years treatment, and 8 healthy controls. Clinical information was collected, predisposing haplotypes were determined; the fecal DNA was extracted, the V4 region of the 16S rRNA gene amplified and 454-pyrosequenced. The newly diagnosed T1D cases had high levels of the genus Bacteroides (p < 0.004), whereas the control group had a gut microbiota dominated by Prevotella. Children with T1D treated for ≥2 years had levels of Bacteroides and Prevotella compared to those of the control group. The gut microbiota of newly diagnosed T1D cases is altered, but whether it is involved in disease causation or is a consequence of host selection remains unclear.
Department of Nutrition and Metabolism, Centro de Investigación en Alimentación y Desarrollo, A.C., Hermosillo, Sonora, México.
María Esther
ME
Mejía-León
Alkek Center for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA.
Joseph F
JF
Petrosino
Alkek Center for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA.
Nadim Jose
NJ
Ajami
1] Laboratory of Microbial Ecology, Department of Biology, University of Puerto Rico, San Juan, Puerto Rico [2] Department of Medicine, New York University School of Medicine, New York, NY, USA.
María Gloria
MG
Domínguez-Bello
Department of Nutrition and Metabolism, Centro de Investigación en Alimentación y Desarrollo, A.C., Hermosillo, Sonora, México.
Ana María Calderón
AM
de la Barca
D000293:Adolescent; D001419:Bacteria / Q000145:classification* / Q000235:genetics*; D016022:Case-Control Studies; D002648:Child; D019295:Computational Biology; D003430:Cross-Sectional Studies; D004269:DNA, Bacterial / Q000235:genetics; D003922:Diabetes Mellitus, Type 1 / Q000382:microbiology*; D005243:Feces / Q000382:microbiology*; D005260:Female; D005500:Follow-Up Studies; D041981:Gastrointestinal Tract / Q000382:microbiology; D006801:Humans; D008297:Male; D008800:Mexico; D064307:Microbiota; D011379:Prognosis; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S