Bacteriophage lambda display systems: developments and applications.
Bacteriophage (phage) Lambda (λ) has played a key historic role in driving our understanding of molecular genetics. The lytic nature of λ and the conformation of its major capsid protein gpD in capsid assembly offer several advantages as a phage display candidate. The unique formation of the λ capsid and the potential to exploit gpD in the design of controlled phage decoration will benefit future applications of λ display where steric hindrance and avidity are of great concern. Here, we review the recent developments in phage display technologies with phage λ and explore some key applications of this technology including vaccine delivery, gene transfer, bio-detection, and bio-control.
School of Pharmacy, University of Waterloo, 200 University Avenue West Waterloo, Kitchener, ON, Canada.
Jessica
J
Nicastro
Katlyn
K
Sheldon
Roderick A
RA
Slavcev
D010582:Bacteriophage lambda / Q000235:genetics*; D061505:Cell Surface Display Techniques / Q000379:methods*; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D015678:Viral Structural Proteins / Q000096:biosynthesis / Q000235:genetics
D008565:Membrane Proteins; D011994:Recombinant Proteins; D015678:Viral Structural Proteins