A lectin from Spatholobus parviflorus inhibits Aspergillus flavus α-amylase: enzyme kinetics and thermodynamic studies.
Aspergillus flavus is a commonly found fungal pathogen which produces structurally related and highly toxic secondary metabolites, aflatoxins. It has been proposed that α-amylase inhibitors may limit the ability of the fungus to produce aflatoxins. Hence, this enzyme is a potent target for the development of antifungal agents. In this study, it was found that Spatholobus parviflorus seed lectin (SPL) can inhibit the growth of A. flavus with a MIC value of 1.5 mg/mL. The enzyme kinetics, molecular modeling and isothermal titration calorimetric studies suggest that SPL can inhibit α-amylase with Ki value of 0.0042 mm. Hence, it is suggested that the antifungal activity of SPL might be partly due to its ability to inhibit the enzyme α-amylase.
Department of Biotechnology & Microbiology, Inter-University Centre for Bioscience, Kannur University, Thalassery Campus, Palayad, 670661, India.
Ignatius
I
Tintu
Joseph
J
Abhilash
Kalarickal V
KV
Dileep
Anu
A
Augustine
Madathilkovilakath
M
Haridas
Chittalakkottu
C
Sadasivan
D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001228:Aspergillosis / Q000188:drug therapy; D001231:Aspergillus flavus / Q000187:drug effects / Q000201:enzymology*; D007887:Fabaceae / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D062105:Molecular Docking Simulation; D037121:Plant Lectins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013816:Thermodynamics; D000516:alpha-Amylases / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism
D000935:Antifungal Agents; D037121:Plant Lectins; D000516:alpha-Amylases
enzyme kinetics; lectin; α-amylase