Influence of various antioxidants added to TCM-199 on post-thaw bovine sperm parameters, DNA integrity and fertilizing ability.
Supplementation of the semen extender with antioxidants did not produce any significant effect on CASA and progressive motilities and sperm motility characteristics, in comparison to the control group (P > 0.05). For sperm acrosome and total abnormalities, TCM-199 supplemented with cysteine (2.60 ± 0.24% and 4.80 ± 0.20%), glutamine (2.80 ± 0.20% and 6.40 ± 0.40%), carnitine (2.60 ± 0.24% and 6.00 ± 0.63%) and methionine (3.40 ± 0.51% and 9.20 ± 0.86%) at doses of 2 mM provided a better protective effect, compared to that of the controls (8.00 ± 0.44 and 15.60 ± 1.895). As regards sperm membrane integrity, supplementation with 2 mM of glutamine and methionine (56.00 ± 1.70% and 62.40 ± 1.78%, respectively) resulted in higher rates, when compared to the control group (41.40 ± 4.74%). According to the results of the COMET assay, only the use of TCM-199 supplemented with 2 mM of cysteine reduced DNA damage and resulted in percentages of sperm with damaged DNA (2.17 ± 0.18%) lower than those of the control group (3.16 ± 0.32%) (P < 0.001). For pregnancy rates, there were no significant differences among the extender groups (P > 0.05).
Erciyes University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Kayseri, Turkey; Genome and Stem Cell Center-GENKOK, Erciyes University, Kayseri, Turkey. Electronic address: sariozkan75@yahoo.com.
Serpil
S
Sarıözkan
Selcuk University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Konya, Turkey. Electronic address: mustafanbucak@yahoo.com.
Mustafa Numan
MN
Bucak
Republic of Turkey Ministry of Food, Agriculture and Livestock, General Directorate of Food and Control, Ankara, Turkey.
Pürhan B
PB
Tuncer
Aksaray University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Aksaray, Turkey.
Serhat
S
Büyükleblebici
Erciyes University, Faculty of Medicine, Department of Basic Sciences, Kayseri, Turkey.
Fazile
F
Cantürk
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002331:Carnitine / Q000494:pharmacology; D002417:Cattle; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology; D003545:Cysteine / Q000494:pharmacology*; D053938:DNA Fragmentation / Q000187:drug effects; D005260:Female; D005306:Fertilization / Q000187:drug effects / Q000502:physiology; D005973:Glutamine / Q000494:pharmacology; D008297:Male; D008715:Methionine / Q000494:pharmacology; D011247:Pregnancy; D012662:Semen Preservation / Q000379:methods*; D013081:Sperm Motility / Q000187:drug effects / Q000502:physiology; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology
D000975:Antioxidants; D003451:Cryoprotective Agents; D005973:Glutamine; D008715:Methionine; D003545:Cysteine; D002331:Carnitine
Antioxidants; Bovine; DNA damage; Freeze–thawing; Sperm parameters