Mutations in calpastatin and μ-calpain are associated with meat tenderness, flavor and juiciness in Hanwoo (Korean cattle): molecular modeling of the effects of substitutions in the calpastatin/μ-calpain complex.
The objective of this study was to evaluate the effects of seven single nucleotide polymorphisms (SNPs) in Calpain 1 and Calpastatin genes previously associated with meat tenderness attributes in other cattle breeds in Korean Hanwoo cattle. The Hanwoo resource population was used to study association of 7 SNPs with beef tenderness, flavor, juiciness, intramuscular fat and shear force. In this association study, CAST:c.182A>G (+0.14, P=0.04) and CAST:c.1985G>C (-0.12, P=0.02) had significant effects on juiciness, but no effects on other traits. In contrast, CAPN1:c.1589G>A was associated with meat tenderness (P=0.01) and juiciness (P=0.04). The CAPN1:c.1589G>A (Val530Ile) SNP marker displayed significant effect on the meat tenderness score which is strongly supported by molecular modeling of the CAPN1:c.1589G>A (Val530Ile) variant that inhibits CAST protein from binding more strongly than the wild-type protein, which may explain its effect on meat tenderness.
Hanwoo Experiment Station, National Institute of Animal Science, RDA, PyeongChang 232-950, Republic of Korea. Electronic address: slee46@korea.kr.
Seung-Hwan
SH
Lee
Animal Genome & Bioinformatics Division, National Institute of Animal Science, RDA, Suwon, Republic of Korea.
Seung-Chang
SC
Kim
Animal Genome & Bioinformatics Division, National Institute of Animal Science, RDA, Suwon, Republic of Korea.
Han-Ha
HH
Chai
Animal Production Research and Development Division, National Institute of Animal Science, RDA, Suwon, Republic of Korea.
Soo-Hyun
SH
Cho
Hanwoo Experiment Station, National Institute of Animal Science, RDA, PyeongChang 232-950, Republic of Korea.
Hyeong-Cheol
HC
Kim
Animal Genome & Bioinformatics Division, National Institute of Animal Science, RDA, Suwon, Republic of Korea.
Dajeong
D
Lim
Animal Genome & Bioinformatics Division, National Institute of Animal Science, RDA, Suwon, Republic of Korea.
Bong-Hwan
BH
Choi
Hanwoo Experiment Station, National Institute of Animal Science, RDA, PyeongChang 232-950, Republic of Korea.
Chang-Gwan
CG
Dang
Hanwoo Experiment Station, National Institute of Animal Science, RDA, PyeongChang 232-950, Republic of Korea.
Aditi
A
Sharma
School of Environment & Rural Science, University of New England, Armidale, NSW 2350, Australia.
Cedric
C
Gondro
Hanwoo Experiment Station, National Institute of Animal Science, RDA, PyeongChang 232-950, Republic of Korea.
Boh-Suk
BS
Yang
Department of Life and Environment, National Institute of Animal Science, RDA, Suwon, Republic of Korea.
Seong-Koo
SK
Hong
D000818:Animals; D001947:Breeding; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002154:Calpain / Q000235:genetics*; D002417:Cattle; D005838:Genotype; D006801:Humans; D008460:Meat / Q000032:analysis*; D008958:Models, Molecular; D009154:Mutation; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D013314:Stress, Mechanical; D013649:Taste; D014867:Water
D002135:Calcium-Binding Proteins; D014867:Water; C033668:calpastatin; D002154:Calpain; C449002:mu-calpain
CAPN1; CAST; Hanwoo; Intramuscular fat; Shear force; Tenderness