A novel fluorescence in situ hybridization test for rapid pathogen identification in positive blood cultures.
A novel molecular beacon-based fluorescence in situ hybridization (FISH) test allowing for the identification of a wide range of bacterial pathogens directly in positive blood cultures (BCs) was evaluated with positive BCs of 152 patients. Depending on the Gram stain, either a Gram-negative or a Gram-positive panel was used. The time to result was 30 min, and the hands-on time was only 10 min. Seven per cent of the cultured microorganisms were not included in the FISH panels; the identification rate of those included was 95.2%. Overall, the FISH test enabled accurate pathogen identification in 88.2% of all cases analysed.
Division of Clinical Microbiology, Department of Laboratory Medicine, Medical University Vienna, Vienna, Austria.
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Makristathis
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Riss
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Hirschl
D001419:Bacteria / Q000145:classification / Q000302:isolation & purification*; D001769:Blood / Q000382:microbiology*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D058889:Molecular Typing; D012680:Sensitivity and Specificity; D018805:Sepsis / Q000097:blood / Q000175:diagnosis* / Q000382:microbiology
Blood culture; fluorescence in situ hybridization; identification; pathogen