Evaluation of long-term antibody responses to two inactivated bovine viral diarrhoea virus (BVDV) vaccines.
The aim of the present study was to determine the serological response of heifers after vaccination with two inactivated bovine viral diarrhoea virus (BVDV) vaccines by means of various ELISA tests. Three dairy farms were selected from the Galicia region of Spain. In each herd, a batch of heifers to be vaccinated for the first time was selected and followed for 15 months. Heifers from farm 1 (n=25) were vaccinated with Vaccine A, whereas heifers from farm 2 (n=16) were vaccinated with Vaccine B. Heifers from farm 3 (n=17), where no BVDV vaccines were used, acted as controls. Blood samples were analyzed periodically for BVDV antibodies, using five commercial ELISAs, based on BVDV p80 antigen or whole virus. At the end of the study, none of the animals vaccinated with Vaccine A seroconverted according to p80 antibody status, whereas up to 80% tested positive by ELISA against whole virus antigen. For the animals vaccinated with Vaccine B, 2/16 animals seroconverted according to p80 antibody ELISAs, whereas all had seroconverted according to the ELISA against whole virus antigen. In most cases, based on the use of ELISAs to detect specific antibodies against the p80 protein, at 15 months post-vaccination with inactivated BVDV vaccines the responses did not seem to interfere with detection of antibody to BVDV infection. However, the finding of a small proportion of vaccinated animals seropositive against BVDV p80 antigen suggests that antibodies that interfere with diagnosis of BVDV infection within the herd could exist, even when using p80 ELISAs.
ADSG Vacasan, Sobreira-Fornás s/n, 27500 Chantada, Spain.
Ana M
AM
González
Laboratorio de Sanidad y Producción Animal de Galicia (LASAPAGA), Av. Madrid 77, 27002 Lugo, Spain.
Ignacio
I
Arnaiz
Unidad de Epidemiología y Sanidad Animal del Instituto de Investigación y Análisis Alimentarios, Facultad de Veterinaria de Lugo, Universidad de Santiago de Compostela, Campus Universitario s/n, 27002 Lugo, Spain.
Eduardo
E
Yus
Laboratorio de Sanidad y Producción Animal de Galicia (LASAPAGA), Av. Madrid 77, 27002 Lugo, Spain.
Carmen
C
Eiras
Unidad de Epidemiología y Sanidad Animal del Instituto de Investigación y Análisis Alimentarios, Facultad de Veterinaria de Lugo, Universidad de Santiago de Compostela, Campus Universitario s/n, 27002 Lugo, Spain.
María
M
Sanjuán
Unidad de Epidemiología y Sanidad Animal del Instituto de Investigación y Análisis Alimentarios, Facultad de Veterinaria de Lugo, Universidad de Santiago de Compostela, Campus Universitario s/n, 27002 Lugo, Spain; Departamento de Anatomía y Producción Animal, Facultad de Veterinaria de Lugo, Universidad de Santiago de Compostela, Campus Universitario s/n, 27002 Lugo, Spain. Electronic address: franciscojavier.dieguez@usc.es.
Francisco J
FJ
Diéguez
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000097:blood / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D010447:Peptide Hydrolases / Q000276:immunology*; D020365:RNA Helicases / Q000276:immunology*; D013030:Spain; D015164:Vaccines, Inactivated / Q000276:immunology; D017361:Viral Nonstructural Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
D000914:Antibodies, Viral; D010447:Peptide Hydrolases; D020365:RNA Helicases; D015164:Vaccines, Inactivated; D017361:Viral Nonstructural Proteins; D014765:Viral Vaccines; C086730:p80 protein, bovine viral diarrhea virus; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
BVDV; Diagnosis; ELISA; Serology; Vaccine