Lateral and axial measurement differences between spectral-domain optical coherence tomography systems.
We assessed the reproducibility of lateral and axial measurements performed with spectral-domain optical coherence tomography (SDOCT) instruments from a single manufacturer and across several manufacturers. One human retina phantom was imaged on two instruments each from four SDOCT platforms: Zeiss Cirrus, Heidelberg Spectralis, Bioptigen SDOIS, and hand-held Bioptigen Envisu. Built-in software calipers were used to perform manual measurements of a fixed lateral width (LW), central foveal thickness (CFT), and parafoveal thickness (PFT) 1 mm from foveal center. Inter- and intraplatform reproducibilities were assessed with analysis of variance and Tukey-Kramer tests. The range of measurements between platforms was 5171 to 5290 μm for mean LW (p<0.001), 162 to 196 μm for mean CFT (p<0.001), and 267 to 316 μm for mean PFT (p<0.001). All SDOCT platforms had significant differences between each other for all measurements, except LW between Bioptigen SDOIS and Envisu (p=0.27). Intraplatform differences were significantly smaller than interplatform differences for LW (p=0.020), CFT (p=0.045), and PFT (p=0.004). Conversion factors were generated for lateral and axial scaling between SDOCT platforms. Lateral and axial manual measurements have greater variance across different SDOCT platforms than between instruments from the same platform. Conversion factors for measurements from different platforms can produce normalized values for patient care and clinical studies.
Duke University, Department of Ophthalmology, Durham, North Carolina 27710.
Francisco A
FA
Folgar
Duke University, Department of Ophthalmology, Durham, North Carolina 27710.
Eric L
EL
Yuan
Duke University, Department of Ophthalmology, Durham, North Carolina 27710 bDuke University, Department of Biomedical Engineering, Durham, North Carolina 27710.
Sina
S
Farsiu
Duke University, Department of Ophthalmology, Durham, North Carolina 27710 bDuke University, Department of Biomedical Engineering, Durham, North Carolina 27710.
Cynthia A
CA
Toth
D001331:Automation; D004867:Equipment Design; D005123:Eye / Q000473:pathology; D005584:Fovea Centralis / Q000473:pathology; D006801:Humans; D055095:Optics and Photonics; D019047:Phantoms, Imaging; D015203:Reproducibility of Results; D012160:Retina / Q000473:pathology*; D012542:Scattering, Radiation; D012984:Software; D041623:Tomography, Optical Coherence / Q000295:instrumentation* / Q000379:methods*