New MADS-box gene in fern: cloning and expression analysis of DfMADS1 from Dryopteris fragrans.
MADS genes encode a family of transcription factors, some of which control the identities of floral organs in flowering plants. Most of the MADS-box genes in fern have been cloned and analyzed in model plants, such as Ceratopteris richardii and Ceratopteris pteridoides. In this study, a new MADS-box gene, DfMADS1(GU385475), was cloned from Dryopteris fragrans (L.) Schott to better understand the role of MADS genes in the evolution of floral organs. The full-length DfMADS1 cDNA was 973 bp in length with a 75 bp 5'-UTR and a 169 bp 3'-UTR. The DfMADS1 protein was predicted to contain a typical MIKC-type domain structure consisting of a MADS domain, a short I region, a K domain, and a C-terminal region. The DfMADS1 protein showed high homology with MADS box proteins from other ferns. Phylogenetic analysis revealed that DfMADS1 belongs to the CRM1-like subfamily. RT-PCR analysis indicated that DfMADS1 is expressed in both the gametophytes and the sporophytes of D. fragrans.
Laboratory of Plant Research, College of Life sciences, Northeast Agricultural University, Harbin, Heilongjiang Province, P. R. China ; Institute of Natural Resources and Ecology, Heilongjiang Academy of Science, Harbin, Heilongjiang Province, P. R. China.
Qingyang
Q
Huang
Laboratory of Plant Research, College of Life sciences, Northeast Agricultural University, Harbin, Heilongjiang Province, P. R. China.
Wenhua
W
Li
Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province, P. R. China.
Ruifeng
R
Fan
Laboratory of Plant Research, College of Life sciences, Northeast Agricultural University, Harbin, Heilongjiang Province, P. R. China.
Ying
Y
Chang
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D029624:Ferns / Q000235:genetics*; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D026161:MADS Domain Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D016415:Sequence Alignment
D018076:DNA, Complementary; D026161:MADS Domain Proteins