Consistency of Bordetella pertussis vaccine seed strains and potency of whole-cell pertussis vaccine still in use in Poland.
In Poland, where the wP vaccine has been used since 1960, pertussis rates increased in the mid-1990s. In 2012, the rate of pertussis recognised by surveillance was unexpectedly found to be two-fold higher than in the previous decade. Quality measures on potency and vaccine working seeds were introduced, to confirm the possible impact of manufacturing inconsistency or potency lowering on the observed increase in pertussis. Shewhart charts on potency values for lots released between 2001 and 2013 did not reveal any significant fluctuations. Working seeds of three vaccine strains used within last decade for wP manufacturing belong to the PFGE group III and were highly related. According to PFGE and SDS-PAGE data, all vaccine strains were found consistent according profiling on the genomic and protein levels. According to the sequencing data, they harboured ptxA2, ptxC1, prn1, fim2-1, fim3-1, tcfA2, ptxP1 and were assigned as MLST-2 type. Other factors apart from vaccine manufacturing inconsistency might be responsible for the increase in pertussis noted in 2012 in Poland.
National Institute of Public Health - National Institute of Hygiene, 24 Chocimska Street, 00-791 Warsaw, Poland.
Monika
M
Zawadka
National Institute of Public Health - National Institute of Hygiene, 24 Chocimska Street, 00-791 Warsaw, Poland.
Ewa
E
Mosiej
National Institute of Public Health - National Institute of Hygiene, 24 Chocimska Street, 00-791 Warsaw, Poland.
Maciej
M
Polak
National Institute of Public Health - National Institute of Hygiene, 24 Chocimska Street, 00-791 Warsaw, Poland.
Katarzyna
K
Krysztopa-Grzybowska
Institute of Sera and Vaccines Biotechnology IBSS BIOMED S.A., 8 Sosnowa Street, 30-224 Cracow, Poland.
Bożena
B
Moskała
National Institute of Public Health - National Institute of Hygiene, 24 Chocimska Street, 00-791 Warsaw, Poland.
Natalia
N
Rokosz-Chudziak
National Institute of Public Health - National Institute of Hygiene, 24 Chocimska Street, 00-791 Warsaw, Poland.
Daniel
D
Rabczenko
National Institute of Public Health - National Institute of Hygiene, 24 Chocimska Street, 00-791 Warsaw, Poland.
Ewa
E
Augustynowicz
National Institute of Public Health - National Institute of Hygiene, 24 Chocimska Street, 00-791 Warsaw, Poland. Electronic address: alutynska@pzh.gov.pl.
Anna
A
Lutyńska
D001886:Bordetella pertussis / Q000276:immunology*; D016521:Electrophoresis, Gel, Pulsed-Field; D004591:Electrophoresis, Polyacrylamide Gel; D010567:Pertussis Vaccine / Q000276:immunology*; D011044:Poland
D010567:Pertussis Vaccine
Bordetella pertussis; Pertussis increase; Potency; Vaccine strains; Whole-cell pertussis vaccine