SiO2 nanoparticles change colour preference and cause Parkinson's-like behaviour in zebrafish.
With advances in the development of various disciplines, there is a need to decipher bio-behavioural mechanisms via interdisciplinary means. Here, we present an interdisciplinary study of the role of silica nanoparticles (SiO2-NPs) in disturbing the neural behaviours of zebrafish and a possible physiological mechanism for this phenomenon. We used adult zebrafish as an animal model to evaluate the roles of size (15-nm and 50-nm) and concentration (300 μg/mL and 1000 μg/mL) in SiO2-NP neurotoxicity via behavioural and physiological analyses. With the aid of video tracking and data mining, we detected changes in behavioural phenotypes. We found that compared with 50-nm nanosilica, 15-nm SiO2-NPs produced greater significant changes in advanced cognitive neurobehavioural patterns (colour preference) and caused potentially Parkinson's disease-like behaviour. Analyses at the tissue, cell and molecular levels corroborated the behavioural results, demonstrating that nanosilica acted on the retina and dopaminergic (DA) neurons to change colour preference and to cause potentially Parkinson's disease-like behaviour.
1] State Key Laboratory of Medicinal Chemical Biology, College of Life Science, Nankai University, Tianjin 300071, China [2].
Xiang
X
Li
1] The Institute of Robotics and Automatic Information Systems, Nankai University, Tianjin 300071, China [2].
Bo
B
Liu
1] The Key Laboratory of Animal Models and Degenerative Diseases, Department of Physiology, School of Medicine, Nankai University, Tianjin, 300071, China [2].
Xin-Le
XL
Li
State Key Laboratory of Medicinal Chemical Biology, College of Life Science, Nankai University, Tianjin 300071, China.
Yi-Xiang
YX
Li
The Institute of Robotics and Automatic Information Systems, Nankai University, Tianjin 300071, China.
Ming-Zhu
MZ
Sun
The Key Laboratory of Animal Models and Degenerative Diseases, Department of Physiology, School of Medicine, Nankai University, Tianjin, 300071, China.
Dong-Yan
DY
Chen
The Institute of Robotics and Automatic Information Systems, Nankai University, Tianjin 300071, China.
Xin
X
Zhao
State Key Laboratory of Medicinal Chemical Biology, College of Life Science, Nankai University, Tianjin 300071, China.
Xi-Zeng
XZ
Feng
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D015153:Blotting, Western; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D003116:Color; D004195:Disease Models, Animal; D007124:Immunoenzyme Techniques; D008124:Locomotion / Q000187:drug effects; D053758:Nanoparticles / Q000633:toxicity*; D020734:Parkinsonian Disorders / Q000139:chemically induced*; D012822:Silicon Dioxide / Q000633:toxicity*; D013550:Swimming; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D015027:Zebrafish / Q000002:abnormalities* / Q000378:metabolism
D012822:Silicon Dioxide; D014446:Tyrosine 3-Monooxygenase