Exacerbation of intestinal brush border enzyme activities and oxidative stress in streptozotocin-induced diabetic rats by monocrotophos.
The present study was undertaken to investigate the potential of monocrotophos (MCP), one of the widely used broad spectrum systemic organophosphorus insecticides (OPI) in India, to alter small intestinal structure and function. Further, its potential to exacerbate diabetes induced alterations in intestinal structure and function was also studied in experimentally induced diabetic rats. Rats were rendered diabetic with an acute dose of streptozotocin (60 mg/kg b.w.). MCP was orally administered at a sublethal dose (1/20 LD50 i.e. 0.9 mg/kg b.w./d) for 15 days to both normal and diabetic rats. MCP significantly increased unit weight of intestine in diabetic rats. MCP alone increased (up to 57%) the activities of intestinal brush border disaccharidases in normal rats and further augmented the enzyme activities in diabetic rats. Similar results were found with intestinal alkaline phosphatase activity. In addition, Na(+)/K(+)-ATPase activity was found to be aggravated in diabetic rats by MCP treatment. Oxidative stress markers showed similar degree of change in both MCP and diabetic rats while MCP aggravated oxidative stress condition in diabetic rats. Scanning electron microscopy and histological analysis of the small intestine revealed increased length of villi, congestion, goblet cell hyperplasia and infiltration of inflammatory cells in MCP and diabetic rats while MCP also induced necrotic lesions in diabetic rats. Collectively, our findings provide evidence that multiple doses of MCP has the propensity to augment diabetes associated intestinal dysfunctions in rats.
Food Protectants and Infestation Control Department, CSIR-Central Food Technological Research Institute, Mysore 570 020, India.
Vismaya
Food Protectants and Infestation Control Department, CSIR-Central Food Technological Research Institute, Mysore 570 020, India. Electronic address: rajini29@yahoo.com.
P S
PS
Rajini
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology* / Q000473:pathology*; D004789:Enzyme Activation / Q000187:drug effects; D004798:Enzymes / Q000378:metabolism*; D007306:Insecticides / Q000633:toxicity; D007413:Intestinal Mucosa / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D007421:Intestine, Small / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D008297:Male; D008855:Microscopy, Electron, Scanning; D008871:Microvilli / Q000187:drug effects* / Q000201:enzymology* / Q000473:pathology / Q000648:ultrastructure; D008999:Monocrotophos / Q000633:toxicity*; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress; D051381:Rats; D013482:Superoxide Dismutase / Q000097:blood
D001786:Blood Glucose; D004798:Enzymes; D007306:Insecticides; D008999:Monocrotophos; D013482:Superoxide Dismutase
Brush border enzymes; Diabetes; Disaccharidases; Monocrotophos; Small intestine