Voltammetric determination of nitrite in meat products using polyvinylimidazole modified carbon paste electrode.
A simple and sensitive voltammetric method was developed to determine the amount of nitrite by using Carbon Paste Electrode (CPE) which is modified with polyvinylimidazole (PVI). A buffer solution of phosphate with a pH 4 value was used in the experiments. The amount of the nitrite-ion was determined by cyclic voltammetry (CV). The electro-chemical behaviour of nitrite-ion was investigated by using CV on the PVI modified CPE. A well-defined oxidation peak was obtained at 0.83 V against a reference Ag/AgCl electrode. Differential pulse voltammetry (DPV) was applied for the calibration plot and for the detection limit. The optimisation procedure was done in two steps: using a two-level factorial design for preliminary evaluation of the contributing factors, and the Box-Behnken Design (BBD) to assess the optimal experimental conditions. These are done with the analysis of 3 different factors in 15 runs of DPV. The optimum conditions are obtained within a linear response range of 5×10(-7)-1×10(-4) mol L(-1). Regression analysis is performed within this range showed the linear equation of y=0.028x+3.93×10(-7) with r(2)=0.9982, and for n=7. Limit of Detection (LOD) was 9×10(-8) mol L(-1) with S/N=3, and Limit of Quantification (LOQ) was 3×10(-7) mol L(-1) with S/N=10. The procedure was used successfully to detect the amount of nitrite in meat products.
Department of Chemistry, Faculty of Science and Letters, Istanbul Technical University, Maslak, Istanbul 34469, Turkey. Electronic address: gyildiz@itu.edu.tr.
Gulcemal
G
Yildiz
Department of Chemistry, Faculty of Science and Letters, Istanbul Technical University, Maslak, Istanbul 34469, Turkey.
Nevin
N
Oztekin
Department of Chemistry, Faculty of Science and Letters, Istanbul Technical University, Maslak, Istanbul 34469, Turkey.
Ayca
A
Orbay
Department of Chemistry, Faculty of Science and Letters, Istanbul Technical University, Maslak, Istanbul 34469, Turkey.
Filiz
F
Senkal
D000818:Animals; D002244:Carbon / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D005520:Food Preservatives / Q000032:analysis*; D007093:Imidazoles / Q000737:chemistry; D008461:Meat Products / Q000032:analysis*; D009573:Nitrites / Q000032:analysis*; D011145:Polyvinyls / Q000737:chemistry; D012756:Sheep
D005520:Food Preservatives; D007093:Imidazoles; D009573:Nitrites; D011145:Polyvinyls; C072974:polyvinylimidazole; D002244:Carbon
Experimental design; Nitrite; Polyvinylimidazole; Voltammetry