New lignans from the bioactive fraction of Sambucus williamsii Hance and proliferation activities on osteoblastic-like UMR106 cells.
Four new lignans (1, 7-9), together with nine known ones, were isolated from the anti-osteoporosis fraction of the extract of Sambucus williamsii Hance which was eluted by 50% and 95% aqueous ethanol over D101 macroporous resin column. Their structures were elucidated by NMR spectroscopic analyses, and the absolute configurations of all compounds were determined by application of circular dichroism method. All the compounds were reported for the first time from the Sambucus genus and firstly studied for their proliferation effects on osteoblastic-like UMR 106 cell. The data showed that compounds 2-9 significantly promoted cell proliferation in some dose, especially compounds 2, 3, 4, 5, and 7 increased osteoblastic cell numbers by 31.3%, 28.3%, 25.6%, 25.1% and 26.0% at 10(-10) M, 10(-10) M, 10(-7) M, 10(-10) M and 10(-10) M, respectively, which suggested that lignans were the components accounting for the bone protective effects of SWH.
Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, China; State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen Research Institute of The Hong Kong Polytechnic University, Shenzhen 518057, China.
Hui-hui
HH
Xiao
Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, China.
Yi
Y
Dai
State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen Research Institute of The Hong Kong Polytechnic University, Shenzhen 518057, China; Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.
Man-sau
MS
Wong
Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, China; Department of Natural Products Chemistry, Shenyang Pharmaceutical University, Shenyang 110016, China. Electronic address: tyaoxs@jnu.edu.cn.
Xin-sheng
XS
Yao
D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002942:Circular Dichroism; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D010006:Osteoblasts / Q000187:drug effects; D018547:Plant Stems / Q000737:chemistry; D051381:Rats; D027923:Sambucus / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D017705:Lignans
Lignan; Proliferation effects; Sambucus williamsii Hance; UMR 106 cell