An environmental Escherichia albertii strain, DM104, induces protective immunity to Shigella dysenteriae in guinea pig eye model.
The environmental Escherichia albertii strain DM104, which cross-reacts serologically with Shigella dysenteriae was assessed for pathogenic properties, immunogenicity, and protective efficacy in different animal models to evaluate it as a vaccine candidate against S. dysenteriae, which causes the severe disease, shigellosis. The DM104 isolate was found to be non-invasive and did not produce any entero- or cyto-toxins. The strain also showed negative results in the mouse lethal activity assay. The non-pathogenic DM104 strain gave, however, a high protective efficacy as an ocularly administered vaccine in the guinea pig eye model against S. dysenteriae type 4 challenge. It also induced a high titer of serum IgG against S. dysenteriae type 4 whole cell lysate and lipopolysaccharide. Taken together, all these results indicate a good potential for the use of the DM104 as a live vaccine candidate against shigellosis.
Department of Microbiology, University of Dhaka, Dhaka, Bangladesh.
Fatema Moni
FM
Chowdhury
Mohammed Ziaur
MZ
Rahman
Sirajul Islam
SI
Khan
Chowdhury Rafiqul
CR
Ahsan
Nils-Kåre
NK
Birkeland
D060433:Administration, Ophthalmic; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D004405:Dysentery, Bacillary / Q000276:immunology / Q000517:prevention & control*; D004783:Environmental Microbiology; D004925:Escherichia / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity; D005128:Eye Diseases / Q000276:immunology / Q000517:prevention & control*; D006168:Guinea Pigs; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D012762:Shigella dysenteriae / Q000276:immunology*; D014611:Vaccination / Q000379:methods*
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D007074:Immunoglobulin G