Anti-oxidative effects of Rooibos tea (Aspalathus linearis) on immobilization-induced oxidative stress in rat brain.
Exposure to chronic psychological stress may be related to increased reactive oxygen species (ROS) or free radicals, and thus, long-term exposure to high levels of oxidative stress may cause the accumulation of oxidative damage and eventually lead to many neurodegenerative diseases. Compared with other organs, the brain appears especially susceptible to excessive oxidative stress due to its high demand for oxygen. In the case of excessive ROS production, endogenous defense mechanisms against ROS may not be sufficient to suppress ROS-associated oxidative damage. Dietary antioxidants have been shown to protect neurons against a variety of experimental neurodegenerative conditions. In particular, Rooibos tea might be a good source of antioxidants due to its larger proportion of polyphenolic compounds. An optimal animal model for stress should show the features of a stress response and should be able to mimic natural stress progression. However, most animal models of stress, such as cold-restraint, electric foot shock, and burn shock, usually involve physical abuse in addition to the psychological aspects of stress. Animals subjected to chronic restraint or immobilization are widely believed to be a convenient and reliable model to mimic psychological stress. Therefore, in the present study, we propose that immobilization-induced oxidative stress was significantly attenuated by treatment with Rooibos tea. This conclusion is demonstrated by Rooibos tea's ability to (i) reverse the increase in stress-related metabolites (5-HIAA and FFA), (ii) prevent lipid peroxidation (LPO), (iii) restore stress-induced protein degradation (PD), (iv) regulate glutathione metabolism (GSH and GSH/GSSG ratio), and (v) modulate changes in the activities of antioxidant enzymes (SOD and CAT).
Adult Stem Cell Research Center, Seoul National University, Seoul, Republic of Korea ; Department of Veterinary Public Health, Laboratory of Stem Cell and Tumor Biology, Seoul National University, Seoul, Republic of Korea.
In-Sun
IS
Hong
Adult Stem Cell Research Center, Seoul National University, Seoul, Republic of Korea ; Department of Veterinary Public Health, Laboratory of Stem Cell and Tumor Biology, Seoul National University, Seoul, Republic of Korea.
Hwa-Yong
HY
Lee
Department of Biomedical Science, Jungwon University, Chungbuk, Korea.
Hyun-Pyo
HP
Kim
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D036903:Aspalathus; D001628:Beverages; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006897:Hydroxyindoleacetic Acid / Q000494:pharmacology; D007103:Immobilization / Q000379:methods; D015227:Lipid Peroxidation / Q000187:drug effects; D023421:Models, Animal; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D013662:Tea
D000975:Antioxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D013662:Tea; D006897:Hydroxyindoleacetic Acid; D005978:Glutathione