Two bioactive compounds from the Chinese scorpion Buthusmartensii Karsch.
Two compounds, 3β-acetoxyl,2,14,22-trihydroxy,19-hydroxymethyl,9α,5β,14β-card-20(22)enolide (1) and 1,2,3,4-tetrahydro-6-hydroxy1-5-pyrimidinecarbox-aldehyde (2), were isolated from arthropods in scorpion, Buthus martensii Karsch, by medium pressure liquid chromatography with silica gel as stationary phase and RP-HPLC analysis technology. The structures were elucidated on the basis of NMR spectroscopic analysis and HR-ESI-MS determination. It was found that (1) is a novel compound and both compounds showed inhibitory activities against the Gram-positive bacteria Bacillus subtilis (minimum inhibitory concentrations = 15 μg/mL), with diameter of inhibition zone at ϕ = 6.0 mm (compound 1) and ϕ = 9.0 mm (compound 2), respectively.
a School of Chemical Engineering and Pharmaceutics, Henan University of Science and Technology , Luoyang , Henan 471023 , P.R. China.
Jiayu
J
Gao
Weiping
W
Yin
Ting
T
Gao
Ruixue
R
Deng
Xin
X
Li
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects; D002298:Cardenolides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002853:Chromatography, Liquid; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D012605:Scorpions / Q000737:chemistry*
C000592003:1,2,3,4-tetrahydro-6-hydroxy1-5-pyrimidinecarbox-aldehyde; C000592002:3beta-acetoxyl,2,14,22-trihydroxy,19-hydroxymethyl,9alpha,5beta,14beta-card-20(22)enolide; D000900:Anti-Bacterial Agents; D002298:Cardenolides
Buthus martensii Karsch; antibacterial activity; cardenolides; insect nature products; scorpion; toxic digitalis aglucon