Trans-membrane transport of fluoranthene by Rhodococcus sp. BAP-1 and optimization of uptake process.
The mechanism of transport of (14)C-fluoranthene by Rhodococcus sp. BAP-1, a Gram-positive bacterium isolated from crude oil-polluted soil, was examined. Our finding demonstrated that the mechanism for fluoranthene travel across the cell membrane in Rhodococcus sp. BAP-1 requires energy. Meanwhile, the transport of fluoranthene involves concurrent catabolism of (14)C, that leading to the generation of significant amount of (14)CO2. Combined with trans-membrane transport dynamic and response surface methodology, a significant influence of temperature, pH and salinity on cellular uptake rate was screened by Plackett-Burman design. Then, Box-Behnken design was employed to optimize and enhanced the trans-membrane transport process. The results predicted by Box-Behnken design indicated that the maximum cellular uptake rate of fluoranthene could be achieve to 0.308μmolmin(-1)mg(-1)·protein (observed) and 0.304μmolmin(-1)mg(-1)·protein (predicted) when the initial temperature, pH and salinity were set at 20°C, 9% and 1%, respectively.
College of Water Sciences, Beijing Normal University, Beijing, China.
Yi
Y
Li
College of Water Sciences, Beijing Normal University, Beijing, China. Electronic address: whongqi310@sohu.com.
Hongqi
H
Wang
College of Water Sciences, Beijing Normal University, Beijing, China.
Fei
F
Hua
College of Water Sciences, Beijing Normal University, Beijing, China.
Mengyuan
M
Su
College of Water Sciences, Beijing Normal University, Beijing, China.
Yicun
Y
Zhao
D001673:Biodegradation, Environmental; D001692:Biological Transport / Q000502:physiology; D002250:Carbon Radioisotopes / Q000378:metabolism; D002681:China; D017931:DNA Primers / Q000235:genetics; D005449:Fluorenes / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D026901:Membrane Transport Proteins / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012240:Rhodococcus / Q000235:genetics / Q000378:metabolism*; D054712:Salinity; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D013696:Temperature
D002250:Carbon Radioisotopes; D017931:DNA Primers; D005449:Fluorenes; D026901:Membrane Transport Proteins; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; C007738:fluoranthene
Fluoranthene transport; Response surface methodology; Rhodococcus sp. BAP-1