An effective strategy for species identification of avian meats using the mitochondrial 12S rRNA gene fragment.
An effective DNA-based molecular method had been used to identify avian species from meats. The method combined the use of a pair of universal primers, which amplified about 440-bp fragment of the mitochondrial 12S rRNA gene. A total of 99 meat samples were tested and 17 haplotypes were identified by DNA sequencing, which representing 14 avian species. One avian species was listed as the national first-grade protected animal in China and the IUCN endangered species. Two avian species were under the national second-grade state protection. The proposed method represents a straightforward and robust method for the accurate identification of avian species that could be used by law enforcement agencies as a tool for the control of illegal trade of meat from protected species.
Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection and.
Lan-Ping
LP
Wang
Rong-Qing
RQ
Geng
Zhong-Quan
ZQ
Liu
D000818:Animals; D001717:Birds / Q000235:genetics*; D002681:China; D056727:Endangered Species; D050259:Genes, Mitochondrial; D035846:Law Enforcement; D008460:Meat; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000235:genetics*; D017422:Sequence Analysis, DNA
D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S
12S rRNA gene; PCR sequencing; avian meats; species identification