[Role of p38 mitogen activated protein kinase on cyclic stretch in human facial hypertrophic scar fibroblasts differentiation into myofibroblasts].
OBJECTIVE
To explore the signal transduction mechanism of p38 mitogen activated protein kinase (p38MAPK) in human facial hypertrophic scar fibroblast (FB) differentiation into myofibroblasts (MFB).


METHODS
Fibroblasts of primary culture were simple randomly assigned into two groups: cyclic stretch (control group) and cyclic stretch pre-treated with SB203580(experimental group). Expression of P-p38MAPK and α-smooth muscle actin (α-SMA) protein were examined using Western blotting and expression of transforming growth factor β1 (TGF-β1) mRNA and α-SMA mRNA were examined using reverse transcription PCR (RT-PCR).


RESULTS
In control group, the expressions of α-SMA, p38MAPK, TGF-β1 mRNA and α-SMA mRNA (0 h: 0.134 ± 0.011, 0.239 ± 0.015, 0.214 ± 0.018, 0.252 ± 0.010; 6 h: 0.152 ± 0.014, 0.287 ± 0.016, 0.288 ± 0.011, 0.277 ± 0.013; 12 h: 0.172 ± 0.017, 0.320 ± 0.017, 0.335 ± 0.013, 0.297 ± 0.006) , were significantly increased with loading time (6 h>0 h; 12 h>0 and 6 h). In experimental group (pre-treated with SB203580), the expressions of α-SMA, p38MAPK, TGF-β1 mRNA,α-SMA mRNA (6 h: 0.116 ± 0.017,0.128 ± 0.016,0.134 ± 0.014,0.163 ± 0.009; 12 h: 0.149 ± 0.013,0.136 ± 0.018,0.144 ± 0.013,0.187 ± 0.010) on corresponding time decreased sharply compared with those in control groups (6, 12 h).


CONCLUSIONS
The human facial hypertrophic scar fibroblasts differentiation in response to cyclic stretch was mediated by p38MAPK phosporylation.
Department of Stomatology, The Huangdao Branch, The Affiliated Hospital of Medical College, Qingdao University, Qingdao Shandong 266555, China.
Qi-cui
QC
Du
Department of Stomatology, The Huangdao Branch, The Affiliated Hospital of Medical College, Qingdao University, Qingdao Shandong 266555, China. Email: wenlinxiao@sina.com.
Wen-lin
WL
Xiao
Department of Stomatology, The Huangdao Branch, The Affiliated Hospital of Medical College, Qingdao University, Qingdao Shandong 266555, China.
Ling-fa
LF
Xue
Department of Stomatology, The Huangdao Branch, The Affiliated Hospital of Medical College, Qingdao University, Qingdao Shandong 266555, China.
Shuang-yi
SY
Wang
Department of Stomatology, The Huangdao Branch, The Affiliated Hospital of Medical College, Qingdao University, Qingdao Shandong 266555, China.
Jin
J
Yue
Department of Stomatology, The Huangdao Branch, The Affiliated Hospital of Medical College, Qingdao University, Qingdao Shandong 266555, China.
Yao-xiang
YX
Xu
D000199:Actins / Q000235:genetics / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D054338:Cell Transdifferentiation; D002478:Cells, Cultured; D002648:Child; D017439:Cicatrix, Hypertrophic / Q000378:metabolism / Q000473:pathology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D008297:Male; D058628:Myofibroblasts / Q000473:pathology*; D010766:Phosphorylation; D011725:Pyridines / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D015398:Signal Transduction; D013314:Stress, Mechanical; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism; D055815:Young Adult; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
C541116:ACTA2 protein, human; D000199:Actins; D004791:Enzyme Inhibitors; D007093:Imidazoles; D011725:Pyridines; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D048051:p38 Mitogen-Activated Protein Kinases; C093642:SB 203580