Origins of the reassortant 2009 pandemic influenza virus through proteotyping with mass spectrometry.
The application of a proteotyping approach employing high resolution mass spectrometry based is shown to be able to determine the gene origin of all major viral proteins in a triple reassortant pandemic 2009 influenza strain. Key to this approach is the identification of unique swine-host-specific signature and indicator peptides that are characteristic of influenza viruses circulating in North American and Eurasian swine herds in the years prior to the 2009 influenza pandemic. These swine-and human pandemic-specific signatures enable the origins of viral proteins in a clinical virus specimen to be determined and such strains to be rapidly and directly differentiated from other co-circulating seasonal influenza viruses from the same period. The proteotyping strategy offers advantages over traditional RT-PCR-based approaches that are currently the mainstay of influenza surveillance at the molecular level.
School of Molecular Bioscience, University of Sydney, Sydney, Australia.
Neil D
ND
Fernandes
Kevin M
KM
Downard
D000595:Amino Acid Sequence; D000818:Animals; D006801:Humans; D007251:Influenza, Human / Q000821:virology; D009975:Orthomyxoviridae / Q000737:chemistry / Q000145:classification*; D009976:Orthomyxoviridae Infections / Q000821:virology*; D058873:Pandemics; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry* / Q000145:classification*; D016865:Reassortant Viruses / Q000737:chemistry / Q000145:classification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013552:Swine; D014764:Viral Proteins / Q000032:analysis / Q000737:chemistry* / Q000145:classification*
D010446:Peptide Fragments; D014764:Viral Proteins
influenza virus; mass spectrometry; pandemic; proteotyping; surveillance