Investigations on the antifungal effect of nerol against Aspergillus flavus causing food spoilage.
The antifungal efficacy of nerol (NEL) has been proved against Aspergillus flavus by using in vitro and in vivo tests. The mycelial growth of A. flavus was completely inhibited at concentrations of 0.8 μ L/mL and 0.1 μ L/mL NEL in the air at contact and vapor conditions, respectively. The NEL also had an evident inhibitory effect on spore germination in A. flavus along with NEL concentration as well as time-dependent kinetic inhibition. The NEL presented noticeable inhibition on dry mycelium weight and synthesis of aflatoxin B1 (AFB1) by A. flavus, totally restraining AFB1 production at 0.6 μ L/mL. In real food system, the efficacy of the NEL on resistance to decay development in cherry tomatoes was investigated in vivo by exposing inoculated and control fruit groups to NEL vapor at different concentration. NEL vapors at 0.1 μ L/mL air concentration significantly reduced artificially contaminated A. flavus and a broad spectrum of fungal microbiota. Results obtained from presented study showed that the NEL had a great antifungal activity and could be considered as a benefit and safe tool to control food spoilage.
College of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, China.
Jun
J
Tian
Guangdong Key Laboratory for Research and Development of Natural Drugs, Department of Pharmacology, Guangdong Medical College, Zhanjiang, Guangdong 524023, China.
Xiaobin
X
Zeng
Key Laboratory of Protection and Utilization of Biological Resources, Tarim University, Alar, Xinjiang 843300, China.
Hong
H
Zeng
College of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, China.
Zhaozhong
Z
Feng
College of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, China.
Xiangmin
X
Miao
College of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, China.
Xue
X
Peng
D000080462:Acyclic Monoterpenes; D016604:Aflatoxin B1 / Q000096:biosynthesis; D000935:Antifungal Agents / Q000494:pharmacology*; D001231:Aspergillus flavus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D005516:Food Microbiology; D018551:Solanum lycopersicum / Q000187:drug effects / Q000382:microbiology; D013172:Spores, Fungal / Q000187:drug effects; D013729:Terpenes / Q000494:pharmacology*
D000080462:Acyclic Monoterpenes; D000935:Antifungal Agents; D013729:Terpenes; D016604:Aflatoxin B1; C007836:geraniol