Differential influence of inositol hexaphosphate on the expression of genes encoding TGF-β isoforms and their receptors in intestinal epithelial cells stimulated with proinflammatory agents.
Transforming growth factor β (TGF-β) is a multifunctional cytokine recognized as an important regulator of inflammatory responses. The effect of inositol hexaphosphate (IP6), a naturally occurring phytochemical, on the mRNA expression of TGF- β1, TGF-β2, TGF-β3 and TβRI, TβRII, and TβRIII receptors stimulated with bacterial lipopolysaccharides (Escherichia coli and Salmonella typhimurium) and IL-1β in intestinal cells Caco-2 for 3 and 12 h was investigated. Real-time qRT-PCR was used to validate mRNAs level of examined genes. Bacterial endotoxin promoted differential expression of TGF-βs and their receptors in a time-dependent manner. IL-1β upregulated mRNA levels of all TGF-βs and receptors at both 3 h and 12 h. IP6 elicited the opposed to LPS effect by increasing downregulated transcription of the examined genes and suppressing the expression of TGF- β1 at 12 h. IP6 counteracted the stimulatory effect of IL-1β on TGF-β1 and receptors expression by decreasing their mRNA levels. IP6 enhanced LPS- and IL-1β-stimulated mRNA expression of TGF-β2 and -β3. Based on these studies it may be concluded that IP6 present in the intestinal milieu may exert immunoregulatory effects and chemopreventive activity on colonic epithelium under inflammatory conditions or during microbe-induced infection/inflammation by modulating the expression of genes encoding TGF-βs and their receptors at transcriptional level.
Department of Biochemistry, Medical University of Silesia, Jedności 8, 41-200 Sosnowiec, Poland.
Małgorzata
M
Kapral
Department of Biochemistry, Medical University of Silesia, Jedności 8, 41-200 Sosnowiec, Poland.
Joanna
J
Wawszczyk
Department of Biochemistry, Medical University of Silesia, Jedności 8, 41-200 Sosnowiec, Poland.
Stanisław
S
Sośnicki
Department of Biochemistry, Medical University of Silesia, Jedności 8, 41-200 Sosnowiec, Poland.
Ludmiła
L
Węglarz
D018938:Caco-2 Cells; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D010833:Phytic Acid / Q000494:pharmacology*; D020033:Protein Isoforms / Q000235:genetics; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000235:genetics*; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology; D053781:Transforming Growth Factor beta2 / Q000494:pharmacology; D053782:Transforming Growth Factor beta3 / Q000494:pharmacology
D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D020033:Protein Isoforms; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D053781:Transforming Growth Factor beta2; D053782:Transforming Growth Factor beta3; D010833:Phytic Acid