Spectroscopic investigation on the interaction of copper porphyrazines and phthalocyanine with human telomeric G-quadruplex DNA.
The G-quadruplex DNA is a novel target for anticancer drug discovery and many scientific groups are investigating interaction of small molecules with G-quadruplex DNA to discover therapeutic agents for cancer. Here, interaction of a phthalocyanine (Cu(PcTs)) and two tetrapyridinoporphyrazines ([Cu(2,3-tmtppa)](4+) and [Cu(3,4-tmtppa)](4+)) with Na(+) and K(+) forms of human telomeric G-quadruplex DNA has been investigated by spectroscopic techniques. The results indicated that interaction of the cationic porphyrazines is remarkably stronger than the anionic phthalocyanine and they presumably bind to the G-quadruplex DNA through end-stacking. Fluorescent intercalator displacement assay implied the displacement ability of the complexes with thiazole orange. In addition, circular dichroism spectra of both quadruplex forms converge to the Na(+) isoform after binding to the porphyrazines. In conclusion, the porphyrazines as the complexes that bind to the G-quadruplex DNA, could be suitable candidates for further investigations about inhibition of telomerase enzyme.
Department of Biological Sciences, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan 451951159, Iran. Electronic address: hasani@iasbs.ac.ir.
Leila
L
Hassani
Department of Biological Sciences, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan 451951159, Iran.
Fatemeh
F
Hakimian
Department of Chemistry, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan 451951159, Iran.
Elham
E
Safaei
D002942:Circular Dichroism; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D054856:G-Quadruplexes; D006801:Humans; D007211:Indoles / Q000737:chemistry*; D054833:Isoindoles; D008665:Metalloporphyrins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D016615:Telomere / Q000737:chemistry*
D007211:Indoles; D054833:Isoindoles; D008665:Metalloporphyrins; D003300:Copper; D004247:DNA; C013647:phthalocyanine
G-quadruplex; Phthalocyanine; Spectroscopy; Telomer