Distinct Ca2+ sources in dendritic spines of hippocampal CA1 neurons couple to SK and Kv4 channels.
Small conductance Ca(2+)-activated K(+) (SK) channels and voltage-gated A-type Kv4 channels shape dendritic excitatory postsynaptic potentials (EPSPs) in hippocampal CA1 pyramidal neurons. Synaptically evoked Ca(2+) influx through N-methyl-D-aspartate receptors (NMDARs) activates spine SK channels, reducing EPSPs and the associated spine head Ca(2+) transient. However, results using glutamate uncaging implicated Ca(2+) influx through SNX-482-sensitive (SNX-sensitive) Cav2.3 (R-type) Ca(2+) channels as the Ca(2+) source for SK channel activation. The present findings show that, using Schaffer collateral stimulation, the effects of SNX and apamin are not mutually exclusive and SNX increases EPSPs independent of SK channel activity. Dialysis with 1,2-bis(o-aminophenoxy)ethane-N'N'N'-tetraacetic acid (BAPTA), application of 4-Aminopyridine (4-AP), expression of a Kv4.2 dominant negative subunit, and dialysis with a KChIPs antibody occluded the SNX-induced increase of EPSPs. The results suggest two distinct Ca(2+) signaling pathways within dendritic spines that link Ca(2+) influx through NMDARs to SK channels and Ca(2+) influx through R-type Ca(2+) channels to Kv4.2-containing channels.
Vollum Institute, Oregon Health & Science University, Portland, OR 97239, USA.
Kang
K
Wang
Department of Physiology, University of South Alabama, Mobile, AL 36688, USA.
Mike T
MT
Lin
Vollum Institute, Oregon Health & Science University, Portland, OR 97239, USA. Electronic address: adelman@ohsu.edu.
John P
JP
Adelman
Department of Obstetrics and Gynecology, Oregon Health & Science University, Portland, OR 97239, USA. Electronic address: mayliej@ohsu.edu.
James
J
Maylie
D015761:4-Aminopyridine / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D001030:Apamin / Q000494:pharmacology; D056547:CA1 Region, Hippocampal / Q000166:cytology*; D002118:Calcium / Q000378:metabolism*; D049229:Dendritic Spines / Q000187:drug effects / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D018274:Electroporation; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000235:genetics; D066298:In Vitro Techniques; D051379:Mice; D009474:Neurons / Q000166:cytology*; D026902:Potassium Channel Blockers / Q000494:pharmacology; D011720:Pyrazoles / Q000494:pharmacology; D051672:Shal Potassium Channels / Q000235:genetics / Q000378:metabolism*; D051640:Small-Conductance Calcium-Activated Potassium Channels / Q000235:genetics / Q000378:metabolism*; D013111:Spider Venoms; D013997:Time Factors
D026902:Potassium Channel Blockers; D011720:Pyrazoles; C115521:SNX 482; D051672:Shal Potassium Channels; D051640:Small-Conductance Calcium-Activated Potassium Channels; D013111:Spider Venoms; C411671:TRAM 34; D001030:Apamin; D015761:4-Aminopyridine; D002118:Calcium