Pharmacokinetic comparison of berberine in rat plasma after oral administration of berberine hydrochloride in normal and post inflammation irritable bowel syndrome rats.
In the present study, post inflammation irritable bowel syndrome (PI-IBS) rats were firstly established by intracolonic instillation of acetic acid with restraint stress. Then the pharmacokinetics of berberine in the rat plasma were compared after oral administration of berberine hydrochloride (25 mg/kg) to normal rats and PI-IBS rats. Quantification of berberine in the rat plasma was achieved by using a sensitive and rapid UPLC-MS/MS method. Plasma samples were collected at 15 different points in time and the pharmacokinetic parameters were analyzed by WinNonlin software. Compared with the normal group, area under the plasma concentration vs. time curve from zero to last sampling time (AUC0-t) and total body clearance (CL/F) in the model group significantly increased or decreased, (2039.49 ± 492.24 vs. 2763.43 ± 203.14; 4999.34 ± 1198.79 vs. 3270.57 ± 58.32) respectively. The results indicated that the pharmacokinetic process of berberine could be altered in PI-IBS pathological conditions.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Dongzhimen Nei Nanxiao Road, Dongcheng District, Beijing 100700, China. gzp4012607@126.com.
Zipeng
Z
Gong
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Dongzhimen Nei Nanxiao Road, Dongcheng District, Beijing 100700, China. chenying0919@hotmail.com.
Ying
Y
Chen
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Dongzhimen Nei Nanxiao Road, Dongcheng District, Beijing 100700, China. zoucheng1218@163.com.
Ruijie
R
Zhang
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Dongzhimen Nei Nanxiao Road, Dongcheng District, Beijing 100700, China. wyh811018@163.com.
Yinghan
Y
Wang
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Dongzhimen Nei Nanxiao Road, Dongcheng District, Beijing 100700, China. yaoxueguoyan@163.com.
Yan
Y
Guo
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Dongzhimen Nei Nanxiao Road, Dongcheng District, Beijing 100700, China. yangqing121328@aliyun.com.
Qing
Q
Yang
Institute of Ethnic Medicine, Southwest University, Chengdu 610041, China. 1986614haixian@163.com.
Haixian
H
Zhang
Guang'an Men Hospital, China Academy of Chinese Medical Sciences, No.5, Beixiange Road, Xicheng District, Beijing 100053, China. dongyu250541@sina.com.
Yu
Y
Dong
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Dongzhimen Nei Nanxiao Road, Dongcheng District, Beijing 100700, China. xgweng75@126.com.
Xiaogang
X
Weng
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Dongzhimen Nei Nanxiao Road, Dongcheng District, Beijing 100700, China. rdou8@sohu.com.
Shuangrong
S
Gao
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Dongzhimen Nei Nanxiao Road, Dongcheng District, Beijing 100700, China. zhuxiaoxin@icmm.ac.cn.
Xiaoxin
X
Zhu
D000284:Administration, Oral; D000818:Animals; D001599:Berberine / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics; D002452:Cell Count; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D006207:Half-Life; D043183:Irritable Bowel Syndrome / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008407:Mast Cells / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry
D001599:Berberine