A targeting drug delivery system for ovarian carcinoma: transferrin modified lipid coated paclitaxel-loaded nanoparticles.
The transferring modified lipid coated PLGA nanoparticles, as a targetable vector, were developed for the targeting delivery of anticancer drugs with paclitaxel (PTX) as a model drug to the ovarian carcinoma, which combines the advantages and avoids disadvantages of polymeric nanoparticles and liposomes in drug delivery. A transmission electron microscopy (TEM) confirmed the lipid coating on the polymeric core. Physicochemical characterizations of TFLPs, such as particle size, zeta potential, morphology, encapsulation efficiency, and in vitro PTX release, were also evaluated. In the cellular uptake study, the TFLPs were more efficiently endocytosed by the A2780 cells with high expression of transferrin receptors than HUVEC cells without the transferrin receptors. Furthermore, the anticancer efficacy of TFLPs on the tumor spheroids was stronger than that of lipid coated PLGA nanoparticles (LPs) and PLGA nanoparticles. In the in vivo study, the TFLPs showed the best inhibition effect of the tumor growth for the ovarian carcinoma-bearing mice. In brief, the TFLPs were proved to be an efficient targeting drug delivery system for ovarian carcinoma.
Key Laboratory of Food Biotechnology of Sichuan Province, School of Bioengineering, Xihua University, Chengdu, P. R. China.
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R
Li
West China School of Pharmacy, Sichuan University, Chengdu, P. R. China.
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Zhang
West China School of Pharmacy, Sichuan University, Chengdu, P. R. China.
X-y
XY
Wang
Key Laboratory of Food Biotechnology of Sichuan Province, School of Bioengineering, Xihua University, Chengdu, P. R. China.
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XG
Chen
Key Laboratory of Food Biotechnology of Sichuan Province, School of Bioengineering, Xihua University, Chengdu, P. R. China.
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YX
He
Key Laboratory of Food Biotechnology of Sichuan Province, School of Bioengineering, Xihua University, Chengdu, P. R. China.
W-y
WY
Yang
Key Laboratory of Food Biotechnology of Sichuan Province, School of Bioengineering, Xihua University, Chengdu, P. R. China.
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X
Yang
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism; D002277:Carcinoma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002626:Chemistry, Pharmaceutical; D004355:Drug Stability; D004705:Endocytosis; D005260:Female; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D046529:Microscopy, Electron, Transmission; D050997:Nanomedicine; D053758:Nanoparticles; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D017239:Paclitaxel / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012995:Solubility; D013499:Surface Properties; D013678:Technology, Pharmaceutical / Q000379:methods; D013997:Time Factors; D014168:Transferrin / Q000737:chemistry / Q000378:metabolism*; D047368:Tumor Burden; D023041:Xenograft Model Antitumor Assays
D000972:Antineoplastic Agents, Phytogenic; D008055:Lipids; D008081:Liposomes; D014168:Transferrin; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D017239:Paclitaxel