[Nuclear criteria for complete dormancy in the shoot apex of Fraxinus excelsior L].
The cytohistological criteria for the vegetative shoot apex dormancy in Fraxinus excelsior L. have been quantitatively established with the aid of 3 techniques: historadiography after incorporation of [(3)H]thymidine, mitotic index and nuclear cytophotometry by the two wavelength method. Nuclear DNA content, mitotic activity and DNA synthesis were compared in 3 different zones(apical zone, lateralzone, rib meristem) of the dormant and non-dormant apices. The periodical break in morphogenetic activity, in contrast to the vegetative period (April to July), is characterized by the absence of zonation and by the fact that meristematic cells remain in the G1 phase of the mitotic cycle. In Fraxinus excelsior L., the meristem dormancy is complete (no DNA synthesis, no mitotic activity and no DNA content greater than 2C).
Laboratoire de Cytologie et Morphogenèse végétales, Institut de Biologie végétale, UER 59, Bâtiment L, 4, place Jussieu, F-75230, Paris Cedex 05, France.
A
A
Cottignies