Association study of SNPs of genes IFNGR1 (rs137854905), GSTT1 (rs71748309), and GSTP1 (rs1695) in gastric cancer development in samples of patient in the northern and northeastern Brazil.
Cancer is a multifactorial disease with a high mortality rate in Brazil and worldwide. Gastric cancer (GC) is considered the fourth type of malignancy more frequent in the population worldwide and the second leading cause of death. This work aimed to evaluate single nucleotide polymorphisms (SNPs) of IFNGR1, GSTT1, and GSTP1 genes samples in gastric cancer. We analyzed 60 samples of gastric cancer, 26 diffuse and 34 intestinal types, totaling 120 alleles for each SNP. The results were obtained by PCR and allele-specific PCR. Statistical analyzes performed using BioEstat 5.0 software, applying the Fisher's exact test and chi-square. Only the SNP gene GSTP1 (rs1695) were significantly associated with gastric cancer in the samples analyzed (χ(2) = 8.73, P < 0.05). Our results suggest that the GSTP1 gene SNP (rs1695) can be considered a risk factor associated with gastric carcinogenesis.
Graduate Program in Cellular and Molecular Biology and Laboratory of Structural Molecular Biology and Oncogenetics-LBMEO, Department of Molecular Biology, Federal University of Paraíba UFPB, 58051-900, Joao Pessoa, Paraiba, Brazil.
Rubistenia Miranda Soares
RM
de Araújo
Cynthia Farias Vieira
CF
de Melo
Fidelis Manes
FM
Neto
Juarez Nóbrega
JN
da Silva
Leonardo Ferreira
LF
Soares
Marilia
M
de Arruda Cardoso Smith
Edilson Carvalho
EC
Sousa
Rommel Mario Rodríguez
RM
Burbano
Arnaldo Correia
AC
de Medeiros
Eleonidas Moura
EM
Lima
D001938:Brazil; D020022:Genetic Predisposition to Disease; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D017471:Receptors, Interferon / Q000235:genetics*; D013274:Stomach Neoplasms / Q000209:etiology / Q000235:genetics*; D000096743:Interferon gamma Receptor
D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; D017471:Receptors, Interferon; D000096743:Interferon gamma Receptor; C413545:glutathione S-transferase T1; C496556:GSTP1 protein, human