Selective extraction of clonazepam from  human plasma and urine samples by  molecularly imprinted polymeric beads.
A molecularly imprinted polymer (MIP) based on free-radical polymerization was prepared with 1-(N,N-biscarboxymethyl)amino-3-allylglycerol and N,N-dimethylacrylamide as functional monomers, N,N-methylene diacrylamide as the cross-linker, copper ion-clonazepam as the template and 2,2-azobis(2-methylbutyronitrile) as the initiator. The imprinted polymer was characterized by Fourier transform infrared spectroscopy, elemental analysis, thermo-gravimetric analysis, and SEM. The MIP of agglomerated microparticles with multipores was used for SPE. The imprinted polymer sorbent was selective for clonazepam. The optimum pH and sorption capacity were 5 and 0.18 mg/g at 20C, respectively. The profile of the drug uptake by the sorbent reflects good accessibility of the active sites in the imprinted polymer sorbent. The MIP-SPE was the most feasible technique for the extraction of clonazepam with a high recovery from human plasma and urine samples.
Homayon Ahmad
HA
Panahi
Ali
A
Mehramizi
Somayeh
S
Ghassemi
Elham
E
Moniri
D002851:Chromatography, High Pressure Liquid; D002998:Clonazepam / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D054802:Molecular Imprinting; D015394:Molecular Structure; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*
D011108:Polymers; D002998:Clonazepam