Comparative study of dietary soy phytoestrogens genistein and equol effects on growth parameters and ovarian development in farmed female beluga sturgeon, Huso huso.
Oocyte maturation in fish is a hormonally regulated process. In the light of long-term oocyte maturation in beluga, the aim of this research was to study the estrogenic effects of different concentrations of soy dietary genistein (GE) and equol (EQ) on the growth performance and ovary development in farmed female Huso huso. Fish were fed with concentrations 0 (control), 0.2, 0.4, 0.8 and 1.6 g of EQ and GE per kg of isoproteic (CP 45 %) and isoenergetic (19.5 MJ/kg) diets during a year. Blood samples and ovary biopsies were collected from each fish seasonally. The main results of the present experimentation are that growth performance was not affected significantly both in GE and EQ (P > 0.05). EQ at concentration 0.4 g/kg had more estrogenic effects than other concentrations of EQ and GE in beluga so that 64 % of fish were matured sexually. Some reproductive indices such as oocyte diameter, testosterone (T) and 17β-estradiol (E₂) increased significantly at EQ 0.4 g/kg at the end of experiment (P < 0.05), while 17α-hydroxy progesterone level (17α-OHP) showed no significant changes at all concentrations. Biochemical indices such as calcium, phosphorous and cholesterol increased at GE concentrations, but decreased at EQ concentrations similarly at the end of experiment. There was a negative relationship between plasma phosphorous and alkaline phosphatase enzyme levels. Based on results, EQ at concentration 0.4 g/kg improved oocyte development more than the other concentrations of GE and EQ, and therefore, it can be used as an additive to diets for inducing ovary development in this species.
The University of Gorgan Agricultural Sciences and Natural Resources, Gorgan, Iran, Ayoub2222002@yahoo.com.
A
A
Yousefi Jourdehi
M
M
Sudagar
M
M
Bahmani
S A
SA
Hosseini
A A
AA
Dehghani
M A
MA
Yazdani
D000818:Animals; D017756:Aquaculture; D019587:Dietary Supplements; D060754:Equol / Q000494:pharmacology*; D005260:Female; D005399:Fishes / Q000097:blood / Q000254:growth & development*; D019833:Genistein / Q000494:pharmacology*; D012739:Gonadal Steroid Hormones / Q000097:blood; D010535:Laparoscopy; D009865:Oocytes / Q000166:cytology / Q000254:growth & development; D010053:Ovary / Q000187:drug effects / Q000254:growth & development*; D048789:Phytoestrogens / Q000494:pharmacology*; D011897:Random Allocation
D012739:Gonadal Steroid Hormones; D048789:Phytoestrogens; D060754:Equol; D019833:Genistein