[Antifungal and antiviral substances of Pseudomonas chlororaphis subsp. aureofaciens strains--components of gaupsin].
Phenazine-1-carboxylic, 2-hydroxy-phenazine-carboxylic acid and 2-hydroxy-phenazine active against phytopathogenic fungi were detected in fermentation broth of Pseudomonas chlororaphis subsp.aureofaciens strains UCM B-111 and UCM B-306--components of insectofungicide biopreparation gaupsin using chromato-mass-spectrometric methods; strain B-306 produced antifungal antibiotic pyrrolnitrin together with phenazines. Supernatants of fermentation broth of P chlororaphis subsp. aureofaciens B-111 and B-306 strains grown in King A medium and exopolymers preparations obtained from these supernatants using evaporation, dialysis and liophylisation were highly active against tobacco mosaic virus (TMV). At a dose of 10 mg/ml they reduced TMV infectivity by 76-96%, at concentrations 1 and 0.1 mg/ml the antiviral effect was decreased to 40-62 and 14-27%, respectively. Dialysis did not influence the antiviral activity of isolated preparations. The latter contained 2-7.6 % of carbohydrates including neutral monosaccharides: fucose, mannose, galactose and glucose.
E A
EA
Kiprianova
V V
VV
Shepelevich
V V
VV
Klochko
A N
AN
Ostapchuk
L D
LD
Varbanets
L B
LB
Skokliuk
A E
AE
Berezkina
L V
LV
Avdeeva
D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005285:Fermentation; D005658:Fungi / Q000187:drug effects* / Q000254:growth & development; D005659:Fungicides, Industrial / Q000737:chemistry; D009005:Monosaccharides / Q000096:biosynthesis / Q000302:isolation & purification; D010619:Phenazines / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D011549:Pseudomonas / Q000737:chemistry* / Q000378:metabolism; D011764:Pyrrolnitrin / Q000302:isolation & purification / Q000378:metabolism; D014026:Nicotiana / Q000821:virology; D014027:Tobacco Mosaic Virus / Q000187:drug effects* / Q000254:growth & development
D000935:Antifungal Agents; D000998:Antiviral Agents; D017077:Culture Media, Conditioned; D005659:Fungicides, Industrial; D009005:Monosaccharides; D010619:Phenazines; C037165:1-phenazinecarboxylic acid; C088012:2-hydroxyphenazine-1-carboxylic acid; C088013:2-hydroxyphenazine; D011764:Pyrrolnitrin