Effect of bone marrow-derived CD11b(+)F4/80 (+) immature dendritic cells on the balance between pro-inflammatory and anti-inflammatory cytokines in DBA/1 mice with collagen-induced arthritis.
OBJECTIVE
To explore the effect of bone marrow-derived CD11b(+)F4/80(+) immature dendritic cells (BM CD11b(+)F4/80(+)iDC) on the balance between pro-inflammatory and anti-inflammatory cytokines in DBA/1 mice with collagen-induced arthritis (CIA).


METHODS
BM CD11b(+)F4/80(+)iDC were induced with rmGM-CSF and rmIL-4, and were identified by the expressions of toll-like receptor 2 (TLR-2), indoleamine 2,3-deoxygenase (IDO), interleukin (IL)-10, transforming growth factor (TGF)-β1 and mixed leukocyte reaction (MLR). CIA was established in DBA/1 mice by immunization with type II collagen. CIA mice were injected intravenously with BM CD11b(+)F4/80(+)iDC three times after immunization. The effect of BM CD11b(+)F4/80(+)iDC on CIA was evaluated by the arthritis index, joint histopathology, body weight, thymus index, thymocytes proliferation, IL-1β, tumor necrosis factor (TNF)-α, IL-17, IL-10 and TGF-β1 levels.


RESULTS
BM CD11b(+)F4/80(+)iDC induced with rmGM-CSF and rmIL-4 expressed high levels of TLR-2, IDO, IL-10 and TGF-β1. Infusion of BM CD11b(+)F4/80(+)iDC in CIA mice significantly reduced the arthritis index and pathological scores of joints, recovered the weight, decreased the thymus index and inhibited thymocyte proliferation. Levels of IL-1β, TNF-α and IL-17 were decreased in BM CD11b(+)F4/80(+)iDC-treated mice.


CONCLUSIONS
BM CD11b(+)F4/80(+)iDC can be induced successfully with rmGM-CSF and rmIL-4. BM CD11b(+)F4/80(+)iDC treatment can ameliorate the development and severity of CIA by regulating the balance between pro-inflammatory cytokines and anti-inflammatory cytokines.
Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Antiinflammatory and Immune Medicine (Anhui Medical University), Ministry of Education, 230032, Hefei, China.
Jingjing
J
Fu
Lingling
L
Zhang
Shanshan
S
Song
Kangliang
K
Sheng
Ying
Y
Li
Peipei
P
Li
Shasha
S
Song
Qingtong
Q
Wang
Jianhong
J
Chu
Wei
W
Wei
D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis*; D001169:Arthritis, Experimental / Q000276:immunology* / Q000628:therapy; D001854:Bone Marrow Cells / Q000502:physiology*; D039481:CD11b Antigen / Q000032:analysis*; D049109:Cell Proliferation; D002478:Cells, Cultured; D016207:Cytokines / Q000502:physiology*; D003713:Dendritic Cells / Q000502:physiology*; D016753:Interleukin-10 / Q000502:physiology; D020381:Interleukin-17 / Q000502:physiology; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D060168:Thymocytes / Q000502:physiology
D000943:Antigens, Differentiation; D039481:CD11b Antigen; D016207:Cytokines; D020381:Interleukin-17; C036763:monocyte-macrophage differentiation antigen; D016753:Interleukin-10