The role of microRNA profiling in prognosticating progression in Ta and T1 urinary bladder cancer.
OBJECTIVE
To analyze microRNA profile in Ta and T1 urinary bladder cancers in combination and separately and to relate this to the risk of later developing higher-stage disease.


METHODS
Formalin-fixed, paraffin-embedded samples of 44 Ta and 42 T1 bladder cancers representing cases with and without stage progression during follow-up were collected and microRNA expression levels were measured by microarray analysis.


RESULTS
In a comparison between the progressors and controls, in the Ta/T1 group, miR-10a-5p and miR-31-5p were differentially expressed. miR-10a-5p was also correlated to time to progression (P = 0.00012). In the subgroup analysis, 3 microRNAs, miR-10a-5p, miR-31-5p, and miR-130a-3p, were differentially expressed among Ta tumors and had a fold change of more than 1.5 (P<0.038). The comparison concerning microRNA expression between the progressors and controls in category T1 cancers revealed no significant differences.


CONCLUSIONS
Profiling revealed that certain microRNAs predicted the risk of developing higher-stage disease among patients with Ta cancers. Lower miR-10a-5p expression in Ta progressing tumors indicates that this microRNA could be important for later malignant potential among this group of patients.
Department of Surgical Sciences, Uppsala University, Uppsala, Sweden. Electronic address: ulrika.segersten@surgsci.uu.se.
Ulrika
U
Segersten
Rosetta Genomics Ltd, Rehovot, Israel.
Yael
Y
Spector
Rosetta Genomics Ltd, Rehovot, Israel.
Yaron
Y
Goren
Rosetta Genomics Ltd, Rehovot, Israel.
Sarit
S
Tabak
Department of Surgical Sciences, Uppsala University, Uppsala, Sweden.
Per-Uno
PU
Malmström
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018450:Disease Progression; D005260:Female; D005500:Follow-Up Studies; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D011446:Prospective Studies; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000473:pathology*
D035683:MicroRNAs
Bladder neoplasms; Case-control study; MicroRNA; Prognosis