Cytotoxic cucurbitane triterpenoids isolated from the rhizomes of Hemsleya amabilis.
Two new cucurbitane triterpenoids, 7β-hydroxycucurbitacin F-25-O-acetate (1) and 2β,3β,20(S),26,27-pentahydroxy-16α,23(S)-epoxycucurbita-5,24-dien-11-one (2) along with eleven known cucurbitane triterpenoids (3-13, resp.) were isolated from the rhizomes of Hemsleya amabilis Diels. The chemical structures of the new isolated compounds were elucidated unambiguously by spectroscopic data analysis. The cytotoxic activities of the isolated cucurbitane triterpenoids were evaluated against the HeLa human cancer cell lines. Hemslecin A (5), the main ingredient of H. amabilis, exhibited the significant cytotoxicity with IC50 value of 0.389 μM.
Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, PR China; College of Pharmacy, Dali University, Dali 671000, PR China.
Xu-Bing
XB
Chen
College of Pharmacy, Dali University, Dali 671000, PR China.
Guang-Yong
GY
Chen
Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, PR China.
Jun-Hua
JH
Liu
Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, PR China.
Min
M
Lei
Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, PR China.
Yu-Hui
YH
Meng
Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, PR China.
De-An
DA
Guo
Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, PR China. Electronic address: xuanliu@mail.shcnc.ac.cn.
Xuan
X
Liu
Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, PR China. Electronic address: lhhu@simm.ac.cn.
Li-Hong
LH
Hu
D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D019662:Cucurbitaceae / Q000737:chemistry*; D054728:Cucurbitacins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D005260:Female; D006367:HeLa Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D010946:Plants, Medicinal; D027343:Rhizome / Q000737:chemistry*; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
C000593509:2beta,3beta,20(S),26,27-pentahydroxy-16alpha,23(S)-epoxycucurbita-5,24-dien-11-one; C000593501:7beta-hydroxycucurbitacin F-25-O-acetate; D000470:Alkaloids; D010936:Plant Extracts; D014315:Triterpenes; C557526:cucurbitacin IIa; D054728:Cucurbitacins
Cucurbitaceae; Cytotoxicity; HeLa cell; Hemsleya amabilis; Triterpenoid