Isolation of cyanidin 3-glucoside from blue honeysuckle fruits by high-speed counter-current chromatography.
Blue honeysuckle fruits are rich in anthocyanins with many beneficial effects such as reduction of the risk of cardiovascular diseases, diabetes and cancers. High-speed counter-current chromatography (HSCCC) was used for the separation of anthocyanin on a preparative scale from blue honeysuckle fruit crude extract with a biphasic solvent system composed of tert-butyl methyl ether/n-butanol/acetonitrile/water/trifluoroacetic acid (2:2:1:5:0.01, v/v) for the first time in this paper. Each injection of 100 mg crude extract yielded 22.8 mg of cyanidin 3-glucoside (C3G) at 98.1% purity. The compound was identified by means of electro-spray ionisation mass (ESI/MS) and (1)H and (13)C nuclear magnetic resonance (NMR) spectra.
College of Bioengineering, Beijing Polytechnic, No.1 Shaoyaoju, Beijing 100029, China.
Liang
L
Chen
College of Bioengineering, Beijing Polytechnic, No.1 Shaoyaoju, Beijing 100029, China. Electronic address: Xiulanxin@163.com.
Xiulan
X
Xin
College of Bioengineering, Beijing Polytechnic, No.1 Shaoyaoju, Beijing 100029, China.
Rong
R
Lan
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, No.15 Beisanhuan East Road, Beijing 100029, China.
Qipeng
Q
Yuan
College of Bioengineering, Beijing Polytechnic, No.1 Shaoyaoju, Beijing 100029, China.
Xiaojie
X
Wang
College of Bioengineering, Beijing Polytechnic, No.1 Shaoyaoju, Beijing 100029, China.
Ye
Y
Li
D000872:Anthocyanins / Q000032:analysis / Q000302:isolation & purification*; D003377:Countercurrent Distribution; D005638:Fruit / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis / Q000302:isolation & purification*; D031239:Lonicera / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification*
D000872:Anthocyanins; D005960:Glucosides; D010936:Plant Extracts; C462279:cyanidin-3-O-beta-glucopyranoside
Blue honeysuckle fruits; Cyanidin 3-glucoside; High-speed counter-current chromatography (HSCCC)