Differential influence of fluoride concentration on the synthesis of bone matrix glycoproteins within mineralizing bone cells in vitro.
OBJECTIVE
This study investigated the influence of fluoride levels on the temporal synthesis of bone-associated glycoproteins, which have been assigned prominent roles in regulating crystal growth, size and shape during the mineralization process.


METHODS
Bone marrow stromal cells were isolated from male Wistar rats and cultured under mineralizing conditions, supplemented with 0 M, 10(-7) M or 10(-5) M sodium fluoride. The presence of bone-associated glycoproteins was examined 2-13 days post-reseeding by immunocytochemical localization. Results: All bone-associated glycoproteins increased in 10(-7) M fluoride, compared to untreated controls, particularly at days 6 and 13 in culture. Conversely, higher 10(-5) M fluoride concentrations decreased glycoprotein levels, compared to controls.


CONCLUSIONS
Results highlight a differential effect of fluoride concentration on glycoprotein synthesis by osteoblasts.
Tissue Engineering and Reparative Dentistry, School of Dentistry, Cardiff University , UK.
Gregory S
GS
Antonarakis
Ryan
R
Moseley
Rachel J
RJ
Waddington
D000818:Animals; D001857:Bone Matrix / Q000187:drug effects*; D002113:Calcification, Physiologic / Q000187:drug effects*; D002327:Cariostatic Agents / Q000008:administration & dosage*; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D006023:Glycoproteins / Q000187:drug effects*; D007150:Immunohistochemistry; D058574:Integrin-Binding Sialoprotein / Q000187:drug effects; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D015675:Osteocalcin / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects; D015676:Osteonectin / Q000187:drug effects; D053495:Osteopontin / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D012969:Sodium Fluoride / Q000008:administration & dosage*
D002327:Cariostatic Agents; D006023:Glycoproteins; C546873:Ibsp protein, rat; D058574:Integrin-Binding Sialoprotein; D015676:Osteonectin; C506897:Spp1 protein, rat; D015675:Osteocalcin; D053495:Osteopontin; D012969:Sodium Fluoride
bone; bone-associated glycoproteins; fluoride; mineralization; osteoblasts