Identification of differentially expressed proteins in porcine alveolar macrophages infected with virulent/attenuated strains of porcine reproductive and respiratory syndrome virus.
The highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) is still a serious threat to the swine industry. However, the pathogenic mechanism of HP-PRRSV remains unclear. We infected host porcine alveolar macrophages (PAMs) with the virulent HuN4 strain and the attenuated HuN4-F112 strain and then utilized fluorescent two-dimensional difference gel electrophoresis (2D-DIGE) to screen for intracellular proteins that were differentially expressed in host cells infected with the two strains. There were 153 proteins with significant different expression (P<0.01) observed, 42 of which were subjected to mass spectrometry, and 24 proteins were identified. PAM cells infected with the virulent strain showed upregulated expression of pyruvate kinase M2 (PKM2), heat shock protein beta-1 (HSPB1), and proteasome subunit alpha type 6 (PSMA6), which were downregulated in cells infected with the attenuated strain. The upregulation of PKM2 provides sufficient energy for viral replication, and the upregulation of HSPB1 inhibits host cell apoptosis and therefore facilitates mass replication of the virulent strain, while the upregulation of PSMA6 facilitates the evasion of immune surveillance by the virus. Studying on those molecules mentioned above may be able to help us to understand some unrevealed details of HP-PRRSV infection, and then help us to decrease its threat to the swine industry in the future.
Division of Swine Infectious Diseases, Shanghai Veterinary Research Institute, CAAS, Shanghai, China.
Yan-Jun
YJ
Zhou
Division of Swine Infectious Diseases, Shanghai Veterinary Research Institute, CAAS, Shanghai, China.
Jian-Ping
JP
Zhu
Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, United States of America.
Tao
T
Zhou
Division of Swine Infectious Diseases, Shanghai Veterinary Research Institute, CAAS, Shanghai, China.
Qun
Q
Cheng
Division of Swine Infectious Diseases, Shanghai Veterinary Research Institute, CAAS, Shanghai, China.
Ling-Xue
LX
Yu
Division of Swine Infectious Diseases, Shanghai Veterinary Research Institute, CAAS, Shanghai, China.
Ya-Xin
YX
Wang
Division of Swine Infectious Diseases, Shanghai Veterinary Research Institute, CAAS, Shanghai, China.
Shen
S
Yang
Division of Swine Infectious Diseases, Shanghai Veterinary Research Institute, CAAS, Shanghai, China.
Yi-Feng
YF
Jiang
Division of Swine Infectious Diseases, Shanghai Veterinary Research Institute, CAAS, Shanghai, China.
Wu
W
Tong
Division of Swine Infectious Diseases, Shanghai Veterinary Research Institute, CAAS, Shanghai, China.
Fei
F
Gao
Division of Swine Infectious Diseases, Shanghai Veterinary Research Institute, CAAS, Shanghai, China.
Hai
H
Yu
Division of Swine Infectious Diseases, Shanghai Veterinary Research Institute, CAAS, Shanghai, China.
Guo-Xin
GX
Li
Division of Swine Infectious Diseases, Shanghai Veterinary Research Institute, CAAS, Shanghai, China.
Guang-Zhi
GZ
Tong
D000818:Animals; D000831:Animals, Newborn; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D063990:Gene Ontology; D016676:Macrophages, Alveolar / Q000378:metabolism* / Q000821:virology*; D058977:Molecular Sequence Annotation; D019318:Porcine Reproductive and Respiratory Syndrome / Q000378:metabolism* / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000472:pathogenicity*; D060066:Protein Interaction Maps; D020543:Proteome / Q000378:metabolism; D040901:Proteomics; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013223:Statistics as Topic; D013552:Swine; D014158:Transcription, Genetic; D057825:Two-Dimensional Difference Gel Electrophoresis; D014774:Virulence
D020543:Proteome