Improving the efficiency of plasmid transformation in Shewanella oneidensis MR-1 by removing ClaI restriction site.
Here we demonstrate that elimination of ClaI restriction site from the sequence of a plasmid DNA increases the efficiency of transformation of Shewanella oneidensis MR-1 significantly. To achieve reliable transformation of S. oneidensis MR-1 plasmids either lacking ClaI site or isolated from primary transformants of S. oneidensis should be used.
Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, NAS of Ukraine, Drahomanov Street 14/16, 79005 Lviv, Ukraine.
Nazarii
N
Rachkevych
IBiTec-S, SB2SM, LMB (UMR CNRS 8221), DSV, CEA, 91191 Gif-sur-Yvette, France.
Kateryna
K
Sybirna
Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, NAS of Ukraine, Drahomanov Street 14/16, 79005 Lviv, Ukraine.
Solomiya
S
Boyko
Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, NAS of Ukraine, Drahomanov Street 14/16, 79005 Lviv, Ukraine.
Yuriy
Y
Boretsky
Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, NAS of Ukraine, Drahomanov Street 14/16, 79005 Lviv, Ukraine; Department of Biotechnology and Microbiology, University of Rzeszow, Zelwerowicza 4, 35-601 Rzeszow, Poland. Electronic address: sibirny@cellbiol.lviv.ua.
Andriy
A
Sibirny
D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D010957:Plasmids; D017384:Sequence Deletion; D020592:Shewanella / Q000201:enzymology* / Q000235:genetics*; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; C032824:ATCGAT-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific
DNA restriction–modification; Shewanella oneidensis; Transformation