Association between serum 25-hydroxyvitamin D and inflammatory cytokines in healthy adults.
Here, we aimed to examine the associations between levels of serum 25-hydroxyvitamin D [25(OH)D] and inflammatory cytokines in healthy Japanese adults. A total of 95 healthy adults (61 women; age range 21-69 years) participated in our study. Fasting blood samples were analyzed for 25(OH)D, 1,25-dihydroxyvitamin D [1,25(OH)2D], interferon-γ (IFN-γ), interleukin-6 (IL-6), and interleukin-17 (IL-17) levels using enzyme-linked immunosorbent assays kits. Total percent body fat was determined by dual energy X-ray absorptiometry (DXA). Moderate to vigorous physical activity (MVPA) was assessed objectively using an activity monitor for 7 days. The mean 25(OH)D concentration was 34.7 nmol/L, and 83 subjects had 25(OH)D concentrations less than 50 nmol/L. Multiple linear regression analysis revealed that serum 25(OH)D level was positively related to plasma IL-17 level (β=0.26, p=0.025), after adjustment for gender, age, vitamin D intake, alcohol consumption, smoking status, and percent body fat. This relationship remained statistically significant (β=0.28, p=0.019) even after additional adjustment for MVPA. However, no significant association was found between serum 25(OH)D level and plasma IFN-γ or IL-6 levels. In conclusion, this study identified a high prevalence of vitamin D deficiency in healthy Japanese adults. Serum 25(OH)D level was positively related to IL-17 level, independent of physical activity.
Graduate School of Sport Sciences, Waseda University, Tokorozawa, Saitama 359-1192, Japan. gzhtxiaomin@ruri.waseda.jp.
Xiaomin
X
Sun
Faculty of Sport Sciences, Waseda University, Tokorozawa, Saitama 359-1192, Japan. zb.cao@aoni.waseda.jp.
Zhen-Bo
ZB
Cao
Graduate School of Sport Sciences, Waseda University, Tokorozawa, Saitama 359-1192, Japan. rockets912@hotmail.com.
Yuping
Y
Zhang
National Institute of Health and Nutrition, Tokyo 162-8636, Japan. ishimi@nih.go.jp.
Yoshiko
Y
Ishimi
Faculty of Sport and Health Science, Ritsumeikan University, Kusatsu, Shiga 525-8577, Japan. tabatai@fc.ritsumei.ac.jp.
Izumi
I
Tabata
Faculty of Sport Sciences, Waseda University, Tokorozawa, Saitama 359-1192, Japan. mhiguchi@waseda.jp.
Mitsuru
M
Higuchi
D000273:Adipose Tissue; D000328:Adult; D000368:Aged; D044466:Asian People; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D007371:Interferon-gamma / Q000097:blood*; D020381:Interleukin-17 / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood*; D008019:Life Style; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D009043:Motor Activity; D015995:Prevalence; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000097:blood; D014808:Vitamin D Deficiency / Q000097:blood / Q000453:epidemiology*; D055815:Young Adult
D020381:Interleukin-17; D015850:Interleukin-6; D014807:Vitamin D; D007371:Interferon-gamma; C104450:25-hydroxyvitamin D