Isolation of Coxiella burnetii from bovines with history of reproductive disorders in India and phylogenetic inference based on the partial sequencing of IS1111 element.
In the present study, a total of 158 blood samples from 148 bovines and 10 dogs having a history of reproductive disorders were screened for Coxiella burnetii by trans-PCR method. In case of bovines, 6.08% (9/148) blood samples comprised of 4.54% (4/88) cattle and 8.33% (5/60) buffaloes turned out to be positive for C. burnetii DNA while all the samples from dogs (10) were found negative. Of the 9 PCR-positive bovine blood samples, the organism could be isolated only from 3 cases of buffaloes by chick embryo inoculation method. Further, to predict the homology and genetic diversity, the recovered C. burnetii isolates designated as Y1, Y3 and Y7 were partially sequenced for IS1111 gene. On phylogenetic analysis, Y3 and Y7 isolates clustered to a common node away from Y1 isolate. This study may enlighten the nature of circulating C. burnetii isolates in different parts of the world. To the best of our knowledge, this appears to be the first report describing phylogenic analysis of C. burnetii isolates based on IS1111 gene sequence.
Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar 243122, India. Electronic address: drdurga.prasad.das@gmail.com.
Durga Prasad
DP
Das
Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar 243122, India.
S V S
SV
Malik
Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar 243122, India.
D B
DB
Rawool
Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar 243122, India.
Samir
S
Das
Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar 243122, India.
Shabu
S
Shoukat
Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar 243122, India.
Ravi Kumar
RK
Gandham
Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar 243122, India.
Sonal
S
Saxena
Division of Veterinary Pathology, Indian Veterinary Research Institute, Izatnagar 243122, India.
R
R
Singh
ICAR Research Complex for Goa, Ela, Old Goa 403402, India.
Swapnil P
SP
Doijad
ICAR Research Complex for Goa, Ela, Old Goa 403402, India.
S B
SB
Barbuddhe
D000034:Abortion, Veterinary / Q000382:microbiology; D000818:Animals; D002020:Buffaloes / Q000382:microbiology*; D002417:Cattle / Q000382:microbiology*; D016997:Coxiella burnetii / Q000235:genetics* / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004285:Dogs; D004715:Endometriosis / Q000150:complications / Q000382:microbiology / Q000662:veterinary; D005260:Female; D007194:India; D010802:Phylogeny; D011247:Pregnancy; D011778:Q Fever / Q000382:microbiology / Q000662:veterinary; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial
Bovines; Coxiella burnetii; IS1111 gene; India; Isolation; Phylogenetic