Plasma antibody profiles in non-human primate tuberculosis.
BACKGROUND
Tuberculosis (TB) in non-human primates (NHPs) is highly contagious, requiring efficient identification of animals infected with Mycobacterium tuberculosis. Tuberculin skin test is usually used but lacks desirable sensitivity/specificity and efficiency.


METHODS
We aimed to develop an immunoassay for plasma antibodies against M. tuberculosis. A key challenge is that not all infected animals contain antibodies against the same M. tuberculosis antigen. Therefore, a multiplex panel of 28 antigens (Luminex(®) -Platform) was developed.


RESULTS
Data revealed antibodies against eight antigens (Rv3875, Rv3875-Rv3874 fusion, Rv3874, Rv0934, Rv3881, Rv1886c, Rv2031, Rv3841) in experimentally infected (M. tuberculosis strains: Erdman and H37Rv) NHPs (rhesus and cynomolgus macaques). In a naturally acquired M. tuberculosis infection, rhesus macaques (n = 15) with lung TB pathology (n = 10) contained antibodies to five additional antigens (Rv0831, Rv2220, Rv0054, Rv1099, and Rv0129c).


CONCLUSIONS
Results suggest that this user-friendly and easily implementable multiplex panel, containing 13 M. tuberculosis antigens, may provide a high-throughput alternative for NHP TB screening.
Center for Comparative Medicine, University of California, Davis, CA, USA.
Resmi
R
Ravindran
Viswanathan V
VV
Krishnan
Rajeev
R
Dhawan
Michelle L
ML
Wunderlich
Nicholas W
NW
Lerche
Joanne L
JL
Flynn
Paul A
PA
Luciw
Imran H
IH
Khan
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D007118:Immunoassay / Q000379:methods; D008252:Macaca fascicularis; D008253:Macaca mulatta; D008863:Microspheres; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D010949:Plasma / Q000276:immunology; D012680:Sensitivity and Specificity; D013047:Specific Pathogen-Free Organisms; D014397:Tuberculosis, Pulmonary / Q000097:blood / Q000662:veterinary*
D000907:Antibodies, Bacterial; D015415:Biomarkers
antibodies; multiplex; tuberculosis