[Inhibitory effect of microwave radiation on proliferation of human pancreatic cancer JF305 cells and its mechanism].
OBJECTIVE
To investigate on the proliferation effect of different intensities 2450 MHz microwave radiation on human pancreatic cancer JF305 cells and its possible mechanism.


METHODS
JF305 cells were radiated by intensity of 2.5, 5.0, 10.0, 15.0 and 20.0 mW/cm2 microwave for 20 min. The proliferation capacity of JF305 was measured by MTT assays, Annexin V-FITC and PI staining was used for detecting cell apoptosis. The activity of Caspase-3 was examined. The expressions of Caspase-3 and HSP 70 protein after the cell treatment with microwave were detected by Western blotting.


RESULTS
After microwave radiation, the proliferation inhibition rates of JF305 cells were significantly higher compared with control group. Annexin V-FITC and PI staining result showed that microwave radiation could induce cell apoptosis. Caspase-3 increased after radiated by microwave, compared with control group (P < 0.05). Results of Western blotting showed that the expression of Caspase-3 and HSP 70 protein increased significantly in different dosage radiation group.


CONCLUSIONS
Microwave radiation can inhibit the proliferation of JF305 cells, the possible mechanism may be related with inducing cell apoptosis by changing of stress level.
Department of Biochemistry and Molecular Biology, Jilin Medical College, Jilin 132013, China. huolizwh@163.com
Wenhe
W
Zhu
Department of Biochemistry and Molecular Biology, Jilin Medical College, Jilin 132013, China.
Wei
W
Zhang
Department of Biochemistry and Molecular Biology, Jilin Medical College, Jilin 132013, China.
Yan
Y
Li
Department of Biochemistry and Molecular Biology, Jilin Medical College, Jilin 132013, China.
Junjie
J
Xu
Department of Biochemistry and Molecular Biology, Jilin Medical College, Jilin 132013, China.
Jun
J
Luo
Department of Biochemistry and Molecular Biology, Jilin Medical College, Jilin 132013, China.
Yanxia
Y
Jiang
Department of Biochemistry and Molecular Biology, Jilin Medical College, Jilin 132013, China.
Xiaojing
X
Lu
Department of Biochemistry and Molecular Biology, Jilin Medical College, Jilin 132013, China.
Shijie
S
Lü
D017209:Apoptosis / Q000528:radiation effects*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000528:radiation effects*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D008872:Microwaves; D010190:Pancreatic Neoplasms / Q000473:pathology*
D018840:HSP70 Heat-Shock Proteins; C505407:CASP3 protein, human; D053148:Caspase 3