Mast cell curve-response in partial Achilles tendon rupture after 830 nm phototherapy.
OBJECTIVE
The aim of this study was to quantify mast cells at different time intervals after partial Achilles tendon rupture in rats treated with low-level laser therapy (LLLT).


BACKGROUND
There is a high incidence of lesions and ruptures in the Achilles tendon that can take weeks and even months to heal completely. As the mast cells help in the healing repair phase, and LLLT has favorable effects on this tissue repair process, study of this modality on the quantity of mastocytes in the ruptured tendon is relevant.


METHODS
Sixty Wistar rats were subjected to partial Achilles' tendon rupture by direct trauma, randomized into 10 groups, and then divided into the group treated with 80 mW aluminum gallium arsenide infrared laser diode, continuous wave, 2.8 W/cm(2) power density, 40 J/cm(2) energy density, and 1.12 J total energy, and the simulation group. Both the groups were subdivided according to the histological assessment period of the sample, either 6 h, 12 h, 24 h, 2 days, or 3 days after the rupture, to quantify the mastocytes in the Achilles' tendon.


RESULTS
The group subjected to LLLT presented a greater quantity of mastocytes in the periods of 6 h, 12 h, 24 h, 2 days, and 3 days after rupture, compared with the simulation groups, but differences were detected between the sample assessment periods only in the simulation group.


CONCLUSIONS
LLLT was shown to increase the quantity of mastocytes in the assessment periods compared with the simulation groups.
1 Department of Human Movement Science, University Federal of São Paulo - UNIFESP - Campus Baixada Santista , Santos, SP, Brazil .
Carlos E
CE
Pinfildi
Érika P Rampazo
ÉP
da Silva
Roberta A C
RA
Folha
Paola C G
PC
Turchetto
Paola Pkp
PP
Monteiro
Arainy
A
Antunes
Bernardo S
BS
Hochman
D000125:Achilles Tendon / Q000293:injuries* / Q000528:radiation effects; D000818:Animals; D007150:Immunohistochemistry; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D008407:Mast Cells / Q000378:metabolism* / Q000528:radiation effects*; D051381:Rats; D017208:Rats, Wistar; D012421:Rupture; D013708:Tendon Injuries / Q000628:therapy; D014945:Wound Healing / Q000502:physiology*