[Determination of mRNA-transcripts and heat shock proteins HSP70 and HSP90 in retina of the adult Spanish Ribbed Newt Pleurodeles waltl].
Expression of genes and heat shock proteins in normal intact retina of the Spanish Ribbed Newt Pleurodeles waltl was studied using polymerase chain reaction, Western blot hybridization, and immunohistochemistry. It was shown that the proteins HSP70 and HSP90, as well as their encoding transcripts of relevant genes, are constitutively expressed in eye tissues. These proteins were distributed differentially, and they were characterized by expression of different levels in the retina: HSP70 dominated in the external retina, while HSP90 dominated in the internal one, in particular, in Muller glial cells and the optic nerve. Transcripts and heat shock proteins HSP70 and HSP90 were also found in the retinal pigment epithelium and eye growth zone.
P P
PP
Avdonin
Yu V
YV
Markitantova
V A
VA
Poplinskaya
E N
EN
Grigoryan
D000818:Animals; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism; D009900:Optic Nerve / Q000378:metabolism; D009928:Organ Specificity; D010857:Pigment Epithelium of Eye / Q000378:metabolism; D010999:Pleurodeles; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D014158:Transcription, Genetic
D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D012333:RNA, Messenger