Protective effect of an immune-modulating diet comprising whey peptides and fermented milk products on indomethacin-induced small-bowel disorders in rats.
OBJECTIVE
An immune-modulating diet (IMD) comprising whey peptides and fermented milk products has produced anti-inflammatory effects in various animal models and has been employed in peri-operative nutritional care. It was investigated the effectiveness of an immune-modulating diet on indomethacin-induced small-bowel disorders.


METHODS
Six-week-old male Sprague-Dawley rats were fed a control diet (control group) or an IMD (IMD group) for 14 days. Thereafter, they were injected with 10 mg/kg/day indomethacin for 2 consecutive days to induce small-bowel disorders. The excretion rate of phenolsulfonphthalein in the urine, bacterial translocation (BT) in the mesenteric lymph nodes (MLNs) and liver, the number of ulcers that formed in the small bowel, and the concentration of cytokines were measured.


RESULTS
The IMD group exhibited significant suppression of increased permeability of the mucosa and decreased BT in the MLNs and liver compared with the control group. The immune-modulating diet group also showed a decrease in ulcer formation and lower concentrations of IL-6 in the ileal tissues compared with the control group.


CONCLUSIONS
These data suggest that the IMD had a protective effect on the small bowel, and may be effective nutritional care in patients with perioperative damage to the small intestine and gastrointestinal disorders.
Nutrition Research Dept., Food Science Research Labs, Meiji Co., Ltd., 540 Naruda, Odawara, Kanagawa 250-0862, Japan. Electronic address: hisae.kume@meiji.com.
Hisae
H
Kume
Nutrition Research Dept., Food Science Research Labs, Meiji Co., Ltd., 540 Naruda, Odawara, Kanagawa 250-0862, Japan.
Keiko
K
Okazaki
Nutrition Research Dept., Food Science Research Labs, Meiji Co., Ltd., 540 Naruda, Odawara, Kanagawa 250-0862, Japan.
Takeshi
T
Takahashi
Nutrition Research Dept., Food Science Research Labs, Meiji Co., Ltd., 540 Naruda, Odawara, Kanagawa 250-0862, Japan.
Taketo
T
Yamaji
D000818:Animals; D018988:Bacterial Translocation / Q000187:drug effects*; D003611:Dairy Products / Q000032:analysis*; D004032:Diet / Q000379:methods*; D015232:Dinoprostone / Q000097:blood; D004195:Disease Models, Animal; D005285:Fermentation; D056747:Immunomodulation; D007213:Indomethacin / Q000009:adverse effects*; D016753:Interleukin-10 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D007410:Intestinal Diseases / Q000139:chemically induced / Q000178:diet therapy*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D008198:Lymph Nodes / Q000187:drug effects / Q000382:microbiology; D008297:Male; D008894:Milk Proteins / Q000008:administration & dosage*; D010539:Permeability; D010637:Phenolsulfonphthalein / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D000067816:Whey Proteins
D015850:Interleukin-6; D008894:Milk Proteins; D014409:Tumor Necrosis Factor-alpha; D000067816:Whey Proteins; D016753:Interleukin-10; D010637:Phenolsulfonphthalein; D015232:Dinoprostone; D007213:Indomethacin
Bacterial translocation; Immune-modulating diet; Indomethacin; Interleukin-6; Intestinal mucosal permeability; Small-bowel disorders