Chitosan-alginate biocomposite containing fucoidan for bone tissue engineering.
Over the last few years, significant research has been conducted in the construction of artificial bone scaffolds. In the present study, different types of polymer scaffolds, such as chitosan-alginate (Chi-Alg) and chitosan-alginate with fucoidan (Chi-Alg-fucoidan), were developed by a freeze-drying method, and each was characterized as a bone graft substitute. The porosity, water uptake and retention ability of the prepared scaffolds showed similar efficacy. The pore size of the Chi-Alg and Chi-Alg-fucoidan scaffolds were measured from scanning electron microscopy and found to be 62-490 and 56-437 µm, respectively. In vitro studies using the MG-63 cell line revealed profound cytocompatibility, increased cell proliferation and enhanced alkaline phosphatase secretion in the Chi-Alg-fucoidan scaffold compared to the Chi-Alg scaffold. Further, protein adsorption and mineralization were about two times greater in the Chi-Alg-fucoidan scaffold than the Chi-Alg scaffold. Hence, we suggest that Chi-Alg-fucoidan will be a promising biomaterial for bone tissue regeneration.
Marine Bioprocess Research Center, Department of Chemistry, Pukyong National University, Busan 608-737, Korea. venkatjchem@pknu.ac.kr.
Jayachandran
J
Venkatesan
Marine Bioprocess Research Center, Department of Chemistry, Pukyong National University, Busan 608-737, Korea. ira@ccmb.res.in.
Ira
I
Bhatnagar
Marine Bioprocess Research Center, Department of Chemistry, Pukyong National University, Busan 608-737, Korea. sknkim@pknu.ac.kr.
Se-Kwon
SK
Kim
D000327:Adsorption; D000464:Alginates / Q000737:chemistry*; D000469:Alkaline Phosphatase / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry*; D001861:Bone Regeneration / Q000187:drug effects; D018786:Bone Substitutes; D002113:Calcification, Physiologic / Q000187:drug effects; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D005612:Freeze Drying; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D011134:Polysaccharides / Q000737:chemistry*; D016062:Porosity; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods*; D014867:Water / Q000737:chemistry
D000464:Alginates; D001672:Biocompatible Materials; D018786:Bone Substitutes; D011134:Polysaccharides; D014867:Water; D048271:Chitosan; C007789:fucoidan; D000469:Alkaline Phosphatase