Evaluation of recombinant LigB antigen-based indirect ELISA and latex agglutination test for the serodiagnosis of bovine leptospirosis in India.
Leptospirosis is a zoonotic disease caused by pathogenic spirochetes of the genus Leptospira, causing febrile infection characterized by multi-organ failure in humans and animals. Leptospiral Ig-like protein B (LigB) is a surface-expressed antigen that mediates host cell invasion or attachment. In this study, N-terminal conserved region of LigB protein (46 kDa) was evaluated for its diagnostic potential to detect anti-leptospiral antibodies in the sera of various animal species. Dot blot analysis revealed immunoreactivity of Leptospira-positive sera of cattle, buffalo, dog, sheep and goat to purified LigB protein. We have analyzed 1126 bovine serum samples, collected from Northern and Eastern part of India, by microscopic agglutination test (MAT) and recombinant LigB (rLigB) based ELISA and latex agglutination test (LAT). The sensitivity of rLigB based ELISA for 554 MAT positive sera was 96.9% and the specificity with 572 MAT negative sera was 91.08% whereas LAT showed sensitivity and specificity of 93.68% and 92.31%, respectively. Kappa values of 0.879 and 0.860 for recombinant antigen based ELISA and LAT indicate excellent agreement with the gold standard serological test, MAT, for the detection of anti-leptospiral antibodies in sera. Further, LAT based on rLigB antigen is a simple and rapid test, suitable for serodiagnosis of leptospirosis under field conditions, owing to its portability and longer shelf life.
Genetic Engineering of Bacteria Lab., Division of Bacteriology & Mycology, Indian Veterinary Research Institute, Izatnagar 243122, India.
Yosef
Y
Deneke
Leptospira Laboratory, Division of Bacteriology & Mycology, Indian Veterinary Research Institute, Izatnagar 243122, India.
T
T
Sabarinath
Genetic Engineering of Bacteria Lab., Division of Bacteriology & Mycology, Indian Veterinary Research Institute, Izatnagar 243122, India.
Neha
N
Gogia
Genetic Engineering of Bacteria Lab., Division of Bacteriology & Mycology, Indian Veterinary Research Institute, Izatnagar 243122, India.
Jonathan
J
Lalsiamthara
Anaerobe Laboratory, Division of Bacteriology & Mycology, Indian Veterinary Research Institute, Izatnagar 243122, India.
K N
KN
Viswas
Genetic Engineering of Bacteria Lab., Division of Bacteriology & Mycology, Indian Veterinary Research Institute, Izatnagar 243122, India. Electronic address: pallab.chaudhuri@gmail.com.
Pallab
P
Chaudhuri
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007194:India; D007841:Latex Fixation Tests / Q000379:methods; D007922:Leptospirosis / Q000175:diagnosis / Q000276:immunology* / Q000662:veterinary*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D015047:Zoonoses / Q000097:blood / Q000175:diagnosis / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; C487006:LigB-m protein, Leptospira interrogans; D011994:Recombinant Proteins
ELISA; Latex agglutination test; Leptospirosis; LigB; Microscopic agglutination test