Effect of hypothermia on radiation-induced micronuclei and delay of cell cycle progression in TK6 cells.
OBJECTIVE
Low temperature (hypothermia) during irradiation leads to a reduced frequency of micronuclei in TK6 cells and it has been suggested that perturbation of cell cycle progression is responsible for this effect. The aim of the study was to test this hypothesis.


METHODS
Human lymphoblastoid TK6 cells were treated by a combination of hypothermia (0.8°C) and ionizing radiation in varying order (hypothermia before, during or after irradiation) and micronuclei were scored. Growth assay and two-dimensional flow cytometry was used to analyze cell cycle kinetics following irradiated of cells at 0.8°C or 37.0°C.


RESULTS
The temperature effect was observed at the level of micronuclei regardless of whether cells were cooled during or immediately before or after the radiation exposure. No indication of cell cycle perturbation by combined exposure to hypothermia and radiation could be detected.


CONCLUSIONS
The protective effect of hypothermia observed at the level of cytogenetic damage was not due to a modulation of cell cycle progression. A possible alternative mechanism and experiments to test it are discussed.
Department of Radiobiology and Immunology, Institute of Biology, Jan Kochanowski University , Kielce , Poland.
Halina
H
Lisowska
Karl
K
Brehwens
Friedo
F
Zölzer
Aneta
A
Wegierek-Ciuk
Joanna
J
Czub
Anna
A
Lankoff
Siamak
S
Haghdoost
Andrzej
A
Wojcik
D002453:Cell Cycle / Q000528:radiation effects*; D002478:Cells, Cultured; D004249:DNA Damage; D006801:Humans; D048629:Micronuclei, Chromosome-Defective / Q000528:radiation effects*; D013696:Temperature