Whole genome amplification of the obligate intracellular pathogen Coxiella burnetii using multiple displacement amplification.
This study demonstrates that whole genome multiple displacement amplification (MDA) is a promising technique for downstream genomic analysis of fastidious obligate intracellular pathogens such as Coxiella burnetii. The MDA technology can help in obtaining sufficient genetic material from highly infectious agent and thus minimizing repeated culturing and associated biohazard.
Sanjay
S
Kumar
Shefali Raj
SR
Gangoliya
Mustapha
M
Berri
Annie
A
Rodolakis
Syed Imteyaz
SI
Alam
D016997:Coxiella burnetii / Q000235:genetics*; D016680:Genome, Bacterial; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*