The Ca(2+)-dependent interaction of calpastatin domain L with the C-terminal tail of the Cav1.2 channel.
To demonstrate the interaction of calpastatin (CS) domain L (CSL) with Cav1.2 channel, we investigated the binding of CSL with various C-terminus-derived peptides at≈free, 100 nM, 10 μM, and 1mM Ca(2+) by using the GST pull-down assay method. Besides binding with the IQ motif, CSL was also found to bind with the PreIQ motif. With increasing [Ca(2+)], the affinity of the CSL-IQ interaction gradually decreased, and the affinity of the CSL-PreIQ binding gradually increased. The results suggest that CSL may bind with both the IQ and PreIQ motifs of the Cav1.2 channel in different Ca(2+)-dependent manners.
Department of Pharmaceutical Toxicology, School of Pharmacy, China Medical University, Shenyang 110001, China.
Wei
W
Sun
Department of Pharmaceutical Toxicology, School of Pharmacy, China Medical University, Shenyang 110001, China; Cardiovascular Institute of China Medical University, Shenyang 110001, China.
Rui
R
Feng
Department of Pharmaceutical Toxicology, School of Pharmacy, China Medical University, Shenyang 110001, China; Cardiovascular Institute of China Medical University, Shenyang 110001, China.
Huiyuan
H
Hu
Department of Pharmaceutical Toxicology, School of Pharmacy, China Medical University, Shenyang 110001, China; Cardiovascular Institute of China Medical University, Shenyang 110001, China.
Feng
F
Guo
Department of Pharmaceutical Toxicology, School of Pharmacy, China Medical University, Shenyang 110001, China; Cardiovascular Institute of China Medical University, Shenyang 110001, China.
Qinghua
Q
Gao
Department of Pharmaceutical Toxicology, School of Pharmacy, China Medical University, Shenyang 110001, China.
Dongxue
D
Shao
Department of Pharmaceutical Toxicology, School of Pharmacy, China Medical University, Shenyang 110001, China.
Dandan
D
Yin
Department of Pharmaceutical Toxicology, School of Pharmacy, China Medical University, Shenyang 110001, China.
Hongmei
H
Wang
Department of Pharmaceutical Toxicology, School of Pharmacy, China Medical University, Shenyang 110001, China; Cardiovascular Institute of China Medical University, Shenyang 110001, China.
Xuefei
X
Sun
Department of Pharmaceutical Toxicology, School of Pharmacy, China Medical University, Shenyang 110001, China; Cardiovascular Institute of China Medical University, Shenyang 110001, China.
Meimi
M
Zhao
Department of Physiology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544, Japan.
Etsuko
E
Minobe
Cardiovascular Institute of China Medical University, Shenyang 110001, China.
Yingxian
Y
Sun
Respiratory Department and Intensive Care Unit, Shengjing Hospital of China Medical University, Shenyang 110004, China.
Guangyu
G
Jiao
Department of Physiology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544, Japan.
Masaki
M
Kameyama
Department of Pharmaceutical Toxicology, School of Pharmacy, China Medical University, Shenyang 110001, China; Cardiovascular Institute of China Medical University, Shenyang 110001, China. Electronic address: lyhao@mail.cmu.edu.cn.
Liying
L
Hao
D020816:Amino Acid Motifs; D000818:Animals; D002118:Calcium / Q000737:chemistry*; D020746:Calcium Channels, L-Type / Q000737:chemistry*; D002135:Calcium-Binding Proteins / Q000737:chemistry*; D006168:Guinea Pigs; D006801:Humans; D010446:Peptide Fragments / Q000737:chemistry; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs
D020746:Calcium Channels, L-Type; D002135:Calcium-Binding Proteins; C419385:L-type calcium channel alpha(1C); D010446:Peptide Fragments; C033668:calpastatin; D002118:Calcium
Affinity; Binding; Ca(2+); Calpastatin; Cav1.2