Application of ultra-high pressure liquid chromatography linear ion-trap orbitrap to qualitative and quantitative assessment of pesticide residues.
The analysis of pesticides residues using a last generation high resolution and high mass accuracy hybrid linear ion trap-Orbitrap mass spectrometer (LTQ-Orbitrap-MS) was explored. Pesticides were extracted from fruits, fish, bees and sediments by QuEChERS and from water by solid-phase with Oasis HLB cartridges. Ultra-high pressure liquid chromatography (UHPLC)-LTQ-Orbitrap mass spectrometer acquired full scan MS data for quantification, and data dependent (dd) MS(2) and MS(3) product ion spectra for identification and/or confirmation. The regression coefficients (r(2)) for the calibration curves (two order of magnitude up to the lowest calibration level) in the study were ≥0.99. The LODs for 54 validated compounds were ≤2ngmL(-1) (analytical standards). The relative standard deviation (RSD), which was used to estimate precision, was always lower than 22%. The recovery of extraction and matrix effects ranged from 58 to 120% and from -92 to 52%, respectively. Mass accuracy was always ≤4ppm, corresponding to a maximum mass error of 1.6millimass units (mmu). This procedure was then successfully applied to pesticide residues in a set of the above-mentioned food and environmental samples. In addition to target analytes, this method enables the simultaneous detection/identification of non-target pesticides, pharmaceuticals, drugs of abuse, mycotoxins, and their metabolites.
Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research (IDAEA-CSIC), Barcelona, Spain.
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Farré
Food and Environmental Safety Research Group (SAMA-UV), Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain. Electronic address: Yolanda.Pico@uv.es.
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Picó
Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research (IDAEA-CSIC), Barcelona, Spain; Institut Català de Recerca de l'Aigua (ICRA), Parc Científic i Tecnològic de la Universitat de Girona, Pic de Peguera, 15, 17003 Girona, Spain.
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Barceló
D000818:Animals; D001516:Bees / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004785:Environmental Pollutants / Q000032:analysis*; D005399:Fishes; D005638:Fruit / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D008460:Meat / Q000032:analysis; D010573:Pesticide Residues / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis
D004785:Environmental Pollutants; D010573:Pesticide Residues; D014874:Water Pollutants, Chemical
Contaminants identification; Environmental samples; Food samples; LTQ-Orbitrap-MS; Pesticide quantification; UHPLC