Characterization of a setup to test the impact of high-amplitude pressure waves on living cells.
The impact of pressure waves on cells may provide several possible applications in biology and medicine including the direct killing of tumors, drug delivery or gene transfection. In this study we characterize the physical properties of mechanical pressure waves generated by a nanosecond laser pulse in a setup with well-defined cell culture conditions. To systematically characterize the system on the relevant length and time scales (micrometers and nanoseconds) we use photon Doppler velocimetry (PDV) and obtain velocity profiles of the cell culture vessel at the passage of the pressure wave. These profiles serve as input for numerical pressure wave simulations that help to further quantify the pressure conditions on the cellular length scale. On the biological level we demonstrate killing of glioblastoma cells and quantify experimentally the pressure threshold for cell destruction.
1] Research Group "Shock Waves in Soft Biological Matter", Fraunhofer Institute for High-Speed Dynamics, Ernst-Mach-Institut, EMI, Eckerstrasse 4, 79104 Freiburg, Germany [2] Department of Stereotactic and Functional Neurosurgery, Neurocenter, University Medical Center Freiburg, Breisacher Straβe 64, 79106 Freiburg, Germany.
Mischa
M
Schmidt
1] Department of Stereotactic and Functional Neurosurgery, Neurocenter, University Medical Center Freiburg, Breisacher Straβe 64, 79106 Freiburg, Germany [2].
Ulf
U
Kahlert
Department of Stereotactic and Functional Neurosurgery, Neurocenter, University Medical Center Freiburg, Breisacher Straβe 64, 79106 Freiburg, Germany.
Johanna
J
Wessolleck
Department of Stereotactic and Functional Neurosurgery, Neurocenter, University Medical Center Freiburg, Breisacher Straβe 64, 79106 Freiburg, Germany.
Donata
D
Maciaczyk
Laser Technology Group, Fraunhofer Institute for High-Speed Dynamics, Ernst-Mach-Institut, EMI, Eckerstrasse 4, 79104 Freiburg, Germany.
Benjamin
B
Merkt
1] Department of Stereotactic and Functional Neurosurgery, Neurocenter, University Medical Center Freiburg, Breisacher Straβe 64, 79106 Freiburg, Germany [2].
Jaroslaw
J
Maciaczyk
Laser Technology Group, Fraunhofer Institute for High-Speed Dynamics, Ernst-Mach-Institut, EMI, Eckerstrasse 4, 79104 Freiburg, Germany.
Jens
J
Osterholz
1] Department of Stereotactic and Functional Neurosurgery, Neurocenter, University Medical Center Freiburg, Breisacher Straβe 64, 79106 Freiburg, Germany [2].
Guido
G
Nikkhah
1] Research Group "Shock Waves in Soft Biological Matter", Fraunhofer Institute for High-Speed Dynamics, Ernst-Mach-Institut, EMI, Eckerstrasse 4, 79104 Freiburg, Germany [2] Department of Chemistry, Faculty of Science, Universität Basel, Klingelbergstrasse 80, CH-4056 Basel, Switzerland.
Martin O
MO
Steinhauser
D001932:Brain Neoplasms / Q000473:pathology*; D003198:Computer Simulation; D005909:Glioblastoma / Q000473:pathology*; D006801:Humans; D017078:Laser-Doppler Flowmetry; D007834:Lasers; D017785:Photons; D011312:Pressure; D014407:Tumor Cells, Cultured