Boron nitride as desalting material in combination with phosphopeptide enrichment in shotgun proteomics.
Hydrophilic peptides in shotgun proteomics have been shown to be problematic in conventional chromatography. Typically, C18 solid phase extraction or peptide traps are used for desalting the sample prior to mass spectrometry analysis, but the capacity to retain hydrophilic peptides is not very high, causing a bias toward more hydrophobic peptides. This is particularly problematic in phosphoproteomic studies. We tested the compatibility of commercially available boron nitride as a novel material for peptide desalting. Boron nitride can be used to recover a wide range of peptides with different physicochemical properties comparable to combined C18 and graphite carbon material.
Department of Ecogenomics and Systems Biology, University of Vienna, A-1090 Vienna, Austria. Electronic address: takeshi.furuhashi@univie.ac.at.
Takeshi
T
Furuhashi
Department of Ecogenomics and Systems Biology, University of Vienna, A-1090 Vienna, Austria.
Ella
E
Nukarinen
GL Sciences, Iruma, Saitama 358-0032, Japan.
Shigenori
S
Ota
Department of Ecogenomics and Systems Biology, University of Vienna, A-1090 Vienna, Austria.
Wolfram
W
Weckwerth
D029681:Arabidopsis Proteins / Q000737:chemistry; D001896:Boron Compounds / Q000737:chemistry*; D010748:Phosphopeptides / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*; D012492:Salts / Q000737:chemistry*
D029681:Arabidopsis Proteins; D001896:Boron Compounds; D010748:Phosphopeptides; D012492:Salts; C017282:boron nitride
Boron nitride; Hydrophilic peptides; Phosphoproteomics; Protein desalting; Sample preparation