Effects of resveratrol and irradiation upon oral squamous cell carcinoma cells.
OBJECTIVE
To evaluate the effects of resveratrol and irradiation on oral squamous cell carcinoma (OSCC).


METHODS
Resveratrol was administered at doses of 5, 10, 25, 50 and 100 µM to PE/CA-PJ15 (OSCC) cultures irradiated with different doses (1, 2.5 and 5 Gy). Effects upon cell viability, apoptosis and migration were evaluated after 24, 48 and 72 h incubation.


RESULTS
After 72 h of incubation, the 100 µM dose of resveratrol induced the greatest decrease in cell viability at all irradiation doses. After 24, 48 and 72 h of incubation, 100 µM of resveratrol induced the greatest cell apoptosis at all irradiation doses. The greatest alterations in the distribution of the G0-G1, G2-M and S phases of the cell cycle were recorded with 50 and 100 µM of resveratrol; after 24, 48 and 72 h of incubation, both these doses resulted in an increase in the S phase, at the expense of the G0-G1 and G2-M phases.


CONCLUSIONS
Resveratrol increases cytotoxic activity in the PE/CA-PJ15 cell line and reduces cell migration capacity, while the combination of resveratrol and irradiation exerts a synergic effect.
Department of Oral Medicine, Faculty Medicine and Dentistry University of Murcia , Murcia , Spain.
Antonio Navarro
AN
Atienzar
Fabio
F
Camacho-Alonso
Pia
P
Lopez-Jornet
D016588:Anticarcinogenic Agents / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000473:pathology* / Q000532:radiotherapy; D002453:Cell Cycle / Q000187:drug effects / Q000528:radiation effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D009062:Mouth Neoplasms / Q000188:drug therapy / Q000473:pathology* / Q000532:radiotherapy; D011879:Radiotherapy Dosage; D000077185:Resveratrol; D016196:S Phase / Q000187:drug effects / Q000528:radiation effects; D013267:Stilbenes / Q000627:therapeutic use*; D013997:Time Factors
D016588:Anticarcinogenic Agents; D013267:Stilbenes; D000077185:Resveratrol
in vitro cell line; irradiation; resveratrol; squamous cell carcinoma