Mesoporous bioactive glass doped-poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) composite scaffolds with 3-dimensionally hierarchical pore networks for bone regeneration.
Scaffolds play a critical role in bone tissue engineering. Composite scaffolds made of biodegradable polymers and bioactive inorganic compounds have demonstrated superior properties in bone defect repair. In this study, highly bioactive, resorbable poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx)-based scaffolds were prepared using combinational 3-dimensional (3D) printing and surface-doping protocol. Structural and morphological characterization of the composite scaffolds demonstrated the homogenous surface-coating of mesoporous bioactive glass (MBG) throughout their porous framework. These hierarchical scaffolds showed bioactivity superior to that of scaffolds made of pure PHBHHx. MBG coating appeared to provide a better environment for human mesenchymal stem cells (hMSCs) attachment, activity, and osteogenic differentiation. Our study indicates that MBG-coated PHBHHx (PHBM) scaffolds may be excellent candidates for use in bone tissue engineering.
The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China; Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, PR China; Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai, PR China.
Shengbing
S
Yang
Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: wjbiomat@163.com.
Jing
J
Wang
The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
Liangji
L
Tang
Shanghai Key Laboratory of Orthopaedic Implant, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, China.
Haiyong
H
Ao
Shanghai Key Laboratory of Orthopaedic Implant, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, China.
Honglue
H
Tan
Shanghai Key Laboratory of Orthopaedic Implant, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, China.
Tingting
T
Tang
The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China; Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, PR China; Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai, PR China. Electronic address: csliu@sh163.net.
Changsheng
C
Liu
D020155:3-Hydroxybutyric Acid / Q000737:chemistry*; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D001861:Bone Regeneration; D002208:Caproates / Q000737:chemistry*; D002516:Ceramics / Q000737:chemistry*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology; D010316:Particle Size; D016062:Porosity; D013499:Surface Properties; D023822:Tissue Engineering; D000094122:Polyhydroxybutyrates
D020155:3-Hydroxybutyric Acid; D001672:Biocompatible Materials; D002208:Caproates; C022107:Bioglass; C115940:poly(3-hydroxybutyrate-co-3-hydroxyhexanoate); D000094122:Polyhydroxybutyrates
Bone regeneration; Mesopore; Scaffolds; Surface modification