Piezoelectric immunosensors for the detection of individual antibiotics and the total content of penicillin antibiotics in foodstuffs.
Piezoelectric immunosensors on the basis of homologous and group-specificantibodies have been developed for detecting penicillin G, ampicillin, and the total content of penicillin antibiotics. The receptor coating of the sensor was obtained by the immobilization of penicillin G or ampicillin hapten-protein conjugates on the polypyrrole film obtained by electropolymerization and activated by glutaraldehyde. The affinity constants and the cross reactivity coefficients have been calculated. This made it possible to estimate the affinity and specificity of the polyclonal and monoclonal antibodies used. The calibration curves are linear in the range of concentrations 2.5-250.0 ng ml(-1) (penicillin G), 2.5-500.0 ng ml(-1) (ampicillin), and 1-500 ng ml(-1) (group of penicillin). The limits of detection are 0.8 ng ml(-1), 3.9 ng ml(-1), which are lower than MRL, established for penicillin antibiotics. The sensors were tested in detecting penicillins in milk, pork, beef, liver.
Lipetsk State Technical University, 30 Moskovskaya St., Lipetsk 398600, Russia. Electronic address: karaseva_nadia@mail.ru.
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Karaseva
Lipetsk State Technical University, 30 Moskovskaya St., Lipetsk 398600, Russia.
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Ermolaeva
D000667:Ampicillin / Q000032:analysis*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002417:Cattle; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D008460:Meat / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D010400:Penicillin G / Q000032:analysis*; D010406:Penicillins / Q000032:analysis*; D013552:Swine
D000900:Anti-Bacterial Agents; D010406:Penicillins; D000667:Ampicillin; D010400:Penicillin G
Antibodies; Foodstuffs; Penicillin; Piezoelectric immunosensor