Detection of histamine in beer by nano extractive electrospray ionization mass spectrometry.
In this study, rapid quantitative detection of histamine in beer was achieved by using nano extractive electrospray ionization mass spectrometry (nano EESI-MS) coupling with standard addition method. Based on the MS(2) experiment, histamine concentrations in three beer samples were determined to be 1.10 ± 0.12 µg/ml, 0.81 ± 0.09 µg/ml and 0.79 ± 0.09 µg/ml. The limit of detection for this method was calculated to be 0.02 µg/ml. These results show that this novel method can be used for direct, rapid and sensitive detection of histamine in beer without any tedious sample pretreatment.
Dalian Polytechnic University, 116034, Dalian, China.
Jiuxiao
J
Cai
Ming
M
Li
Xingchuang
X
Xiong
Xiang
X
Fang
Ruifeng
R
Xu
D001515:Beer / Q000032:analysis*; D006632:Histamine / Q000032:analysis* / Q000737:chemistry; D057230:Limit of Detection; D016014:Linear Models; D036103:Nanotechnology / Q000379:methods*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D006632:Histamine
ambient ionization; beer; extractive electrospray ionization; histamine; mass spectrometry