During cooled storage the extender influences processed autophagy marker light chain 3 (LC3B) of stallion spermatozoa.
To investigate the role of the processed autophagy marker light chain 3 (LC3B) protein in sperm survival in stallion semen processing during cooled storage, split ejaculates were diluted in two different extenders, KMT and INRA 96, and LC3B processing and sperm quality evaluated during incubation at 5°C for five days. After 3 days of incubation there was a drop in total motility in both extenders, although the percentage of progressive motile sperm was greater (P<0.05) in samples extended in INRA96. On Day 5 of cooled storage all sperm parameters decreased significantly independent of the extender, however, samples extended in INRA 96 maintained motility values while those extended in KMT had a further decrease in motility compared with data collected on Day 3 of incubation. The percentage of live sperm decreased over the time of incubation, but only in samples incubated in KMT. The extender had a marked effect in LC3B processing during cooled storage. Spermatozoa maintained in KMT extender did not exhibit LC3B processing, while in spermatozoa incubated in INRA96 there was an increase (P<0.01) in LC3B processing after 5 days of cooled storage. Stallion spermatozoa experience LC3B turnover during cooled storage, however, the extent depends on the extender used. Apparently LC3B turnover is associated with enhanced survival.
Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura Cáceres, Cáceres, Spain.
J M Gallardo
JM
Bolaños
Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura Cáceres, Cáceres, Spain.
A Miró
AM
Morán
Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura Cáceres, Cáceres, Spain.
C M Balao
CM
da Silva
Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura Cáceres, Cáceres, Spain.
M Plaza
MP
Dávila
Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura Cáceres, Cáceres, Spain.
P Martín
PM
Muñoz
Department of Physiology, Faculty of Veterinary Medicine, University of Extremadura, Cáceres, Spain.
I M
IM
Aparicio
Department of Physiology, Faculty of Veterinary Medicine, University of Extremadura, Cáceres, Spain.
J A
JA
Tapia
Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura Cáceres, Cáceres, Spain.
C Ortega
CO
Ferrusola
Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura Cáceres, Cáceres, Spain. Electronic address: fjuanpvega@unex.es.
F J
FJ
Peña
D000818:Animals; D001343:Autophagy; D015415:Biomarkers / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D003080:Cold Temperature; D006736:Horses / Q000378:metabolism; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D055101:Semen Analysis / Q000662:veterinary; D012662:Semen Preservation / Q000379:methods* / Q000662:veterinary; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism*
D015415:Biomarkers; D008869:Microtubule-Associated Proteins; D019188:Organ Preservation Solutions
CASA; Flow cytometry; LC3B; Sperm; Stallion