Maspin is not required for embryonic development or tumour suppression.
Maspin (SERPINB5) is accepted as an important tumour suppressor lost in many cancers. Consistent with a critical role in development or differentiation maspin knockout mice die during early embryogenesis, yet clinical data conflict on the prognostic utility of maspin expression. Here to reconcile these findings we made conditional knockout mice. Surprisingly, maspin knockout embryos develop into overtly normal animals. Contrary to original reports, maspin re-expression does not inhibit tumour growth or metastasis in vivo, or influence cell migration, invasion or survival in vitro. Bioinformatic analyses reveal that maspin is not commonly under-expressed in cancer, and that perturbation of genes near maspin may in fact explain poor survival in certain patient cohorts with low maspin expression.
Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Victoria 3800, Australia.
Sonia S Y
SS
Teoh
Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Victoria 3800, Australia.
Jessica
J
Vieusseux
Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Victoria 3800, Australia.
Monica
M
Prakash
Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Victoria 3800, Australia.
Susan
S
Berkowicz
Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Victoria 3800, Australia.
Jennii
J
Luu
Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Victoria 3800, Australia.
Catherina H
CH
Bird
1] Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Victoria 3800, Australia [2] Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, School of Biomedical Sciences, Monash University, Victoria 3800, Australia.
Ruby H P
RH
Law
1] Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Victoria 3800, Australia [2] Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, School of Biomedical Sciences, Monash University, Victoria 3800, Australia.
Carlos
C
Rosado
Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Victoria 3800, Australia.
John T
JT
Price
1] Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Victoria 3800, Australia [2] Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, School of Biomedical Sciences, Monash University, Victoria 3800, Australia.
James C
JC
Whisstock
Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Victoria 3800, Australia.
Phillip I
PI
Bird
D000818:Animals; D045744:Cell Line, Tumor; D047108:Embryonic Development / Q000502:physiology*; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D009369:Neoplasms / Q000503:physiopathology*; D015843:Serpins / Q000502:physiology*
C085344:SERPIN-B5; D015843:Serpins