A simple method for the determination of reduction potentials in heme proteins.
We describe a simple method for the determination of heme protein reduction potentials. We use the method to determine the reduction potentials for the PAS-A domains of the regulatory heme proteins human NPAS2 (Em=-115 mV ± 2 mV, pH 7.0) and human CLOCK (Em=-111 mV ± 2 mV, pH 7.0). We suggest that the method can be easily and routinely applied to the determination of reduction potentials across the family of heme proteins.
Department of Chemistry, Henry Wellcome Building, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom.
Igor
I
Efimov
Department of Chemistry, Henry Wellcome Building, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom.
Gary
G
Parkin
Department of Chemistry, Henry Wellcome Building, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom.
Elizabeth S
ES
Millett
Department of Chemistry, Henry Wellcome Building, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom.
Jennifer
J
Glenday
Department of Chemistry, Henry Wellcome Building, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom.
Cheuk K
CK
Chan
Department of Chemistry, Henry Wellcome Building, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom.
Holly
H
Weedon
Department of Chemistry, Henry Wellcome Building, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom.
Harpreet
H
Randhawa
Department of Biochemistry, Henry Wellcome Building, University of Leicester, University Road, Leicester LE1 9HN, United Kingdom.
Jaswir
J
Basran
Department of Chemistry, Henry Wellcome Building, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom. Electronic address: emma.raven@le.ac.uk.
Emma L
EL
Raven
D051792:Basic Helix-Loop-Helix Proteins / Q000737:chemistry*; D056926:CLOCK Proteins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006418:Heme / Q000737:chemistry*; D006420:Hemeproteins / Q000737:chemistry*; D006801:Humans; D009419:Nerve Tissue Proteins / Q000737:chemistry*; D010078:Oxazines / Q000737:chemistry; D010084:Oxidation-Reduction; D013057:Spectrum Analysis
D051792:Basic Helix-Loop-Helix Proteins; D056926:CLOCK Proteins; D005456:Fluorescent Dyes; D006418:Heme; D006420:Hemeproteins; D009419:Nerve Tissue Proteins; D010078:Oxazines; C104264:NPAS2 protein, human; C008619:Nile Blue; C538943:CLOCK protein, human
Heme; Heme protein; Redox; Reduction potential