Growth inhibition and differences in protein profiles in azadirachtin-treated Drosophila melanogaster larvae.
Azadirachtin A is a very effective biopesticide widely used in insect pest control. It has strong antifeeding and growth inhibitory activity against most insects, however, its mode of action is still unclear. Proteomic experiments using 2DE indicate significant effects of Azadirachtin A on the amount of proteins related to growth inhibition in Drosophila melanogaster larvae. Twenty-one spots with different intensity in azadirachtin-treated larvae were identified. These proteins are involved in cytoskeletal organization, transcription and translation, hormonal regulation, and energy metabolism. Protein network analysis reveals heat shock protein 23 to be a potential target of azadirachtin. These results provide new insights into understanding the mechanism of growth inhibition in insects in response to azadirachtin.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of Education, South China Agricultural University, Guangzhou, P.R. China.
Hao
H
Wang
Duo
D
Lai
Mei
M
Yuan
Hanhong
H
Xu
D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005786:Gene Expression Regulation / Q000187:drug effects; D007306:Insecticides / Q000378:metabolism*; D007814:Larva / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D036701:Limonins / Q000378:metabolism*; D060066:Protein Interaction Maps; D040901:Proteomics / Q000379:methods
D029721:Drosophila Proteins; D007306:Insecticides; D036701:Limonins; C010329:azadirachtin
Azadirachtin A; Drosophila; Mode of action; Proteomics