Comparison on serum biomarkers for anovulatory and ovulatory dysfunctional uterine bleeding in Lizu females.
OBJECTIVE
To screen, identify, and compare the serum biomarkers between anovulatory dysfunctional uterine bleeding (ADUB) and ovulatory dysfunctional uterine bleeding (ODUB) in Lizu females.


METHODS
The subjects included 128 ADUB patients, 63 ODUB patients, and 93 controls. The serum and supernate of the subjects' mense were collected and stored at -80 °C until use. Differential proteins in the sera of three groups were screened using surface-enhanced laser desorption ionization time-of-flight mass spectrometry. The screened proteins were then identified by tricine-SDS-PAGE gel and spectrometry. Protein expression levels in the menses of ADUB, ODUB, and control subjects were determined using ELISA, RT-PCR, and Western blotting. SPSS 14.1 was used for statistical analysis and chart drawing (α = 0.05).


RESULTS
Three differential protein peaks with peak values of 11.80, 13.59, and 14.68 km/z were screened and identified as serum amyploid protein A (SAA), vascular endothelial growth factor, and vitamin K epoxide reductase, respectively. The SAA was highly expressed in the menses of ADUB and ODUB patients but poorly expressed in the controls. The vascular endothelial growth factor was highly expressed in the menses of ODUB and controls but poorly expressed in ADUB patients. Meanwhile, the vitamin K epoxide reductase was highly expressed in the menses of ADUB and control subjects but poorly expressed in ODUB patients.


CONCLUSIONS
The SAA is the common serum biomarker of ADUB and ODUB. ADUB may be related to angiogenesis impairment, whereas ODUB may be associated with blood coagulation disruption.
Department of Endocrinology, The First Affiliated Hospital, University of South China, Hengyang 421001, China; Department of Gynecology and Obstetrics, Hainan Provincial People's Hospital, Haikou 570311, China.
Shan
S
Bao
Department of Gynecology and Obstetrics, Hainan Provincial People's Hospital, Haikou 570311, China.
Shu-Ying
SY
Yang
Department of Surgery, Hainan Provincial People's Hospital, Haikou 570311, China.
Zhuo-Ri
ZR
Li
Department of Endocrinology, The First Affiliated Hospital, University of South China, Hengyang 421001, China. Electronic address: gb_wen@aliyun.com.
Ge-Bo
GB
Wen
D000328:Adult; D000704:Analysis of Variance; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002681:China; D005260:Female; D006801:Humans; D008796:Metrorrhagia / Q000097:blood*; D008875:Middle Aged; D000685:Serum Amyloid A Protein / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000097:blood; D064417:Vitamin K Epoxide Reductases / Q000097:blood
D015415:Biomarkers; D000685:Serum Amyloid A Protein; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D064417:Vitamin K Epoxide Reductases
Anovulatory dysfunctional uterine bleeding; Biomarker; Serum amyploid protein A; Vascular endothelia growth factor; Vitamin K epoxide reductase