[Screening of urinary biomarkers in patients with type 2 diabetes mellitus].
OBJECTIVE
To screen the biomarkers of the middle-aged type 2 diabetes (T2DM) in urine based on metabolomics.


METHODS
First, both the metabolite profiles of morning urine sample in T2DM and control group were obtained by liquid chromatography-mass spectrometry (LC-MS). Second, an automated compound (feature) extraction algorithm was employed for processing background subtracted single MS data. Then principal component analysis (PCA) and t-Test analysis was followed after preprocessing on the extractive data. Third, identifying the potential biomarkers that show significant difference between the two groups by searching the database online and comparing the results of MS/MS experiments to those available in literature and those standards.


RESULTS
Three endogenous metabolites, among which tryptophan, kynurenic acid, and shown down-regulation while kynurenine shown up-regulation in T2DM, significantly differentiate between T2DM and control group.


CONCLUSIONS
Tryptophan, kynurenic acid and kynurenine may be considered as the potentail biomarkers related to T2DM.
MOE Key Lab of Environment and Health, Institute of Environmental Medicine, School of Public Health, Tongji Medical College, Huazhong University of Science, Wuhan 430030, China.
Han
H
Fu
MOE Key Lab of Environment and Health, Institute of Environmental Medicine, School of Public Health, Tongji Medical College, Huazhong University of Science, Wuhan 430030, China.
Xiaoli
X
Liu
MOE Key Lab of Environment and Health, Institute of Environmental Medicine, School of Public Health, Tongji Medical College, Huazhong University of Science, Wuhan 430030, China.
Wei
W
Yu
MOE Key Lab of Environment and Health, Institute of Environmental Medicine, School of Public Health, Tongji Medical College, Huazhong University of Science, Wuhan 430030, China.
Lijia
L
Zhu
MOE Key Lab of Environment and Health, Institute of Environmental Medicine, School of Public Health, Tongji Medical College, Huazhong University of Science, Wuhan 430030, China.
Dan
D
Zheng
MOE Key Lab of Environment and Health, Institute of Environmental Medicine, School of Public Health, Tongji Medical College, Huazhong University of Science, Wuhan 430030, China.
Jun
J
Wang
D015415:Biomarkers / Q000652:urine*; D003924:Diabetes Mellitus, Type 2 / Q000652:urine*; D005260:Female; D006801:Humans; D007736:Kynurenic Acid / Q000652:urine; D007737:Kynurenine / Q000652:urine; D008297:Male; D008875:Middle Aged; D014364:Tryptophan / Q000652:urine
D015415:Biomarkers; D007737:Kynurenine; D014364:Tryptophan; D007736:Kynurenic Acid