The RSC chromatin remodeling complex has a crucial role in the complete remodeler set for yeast PHO5 promoter opening.
Although yeast PHO5 promoter chromatin opening is a founding model for chromatin remodeling, the complete set of involved remodelers remained unknown for a long time. The SWI/SNF and INO80 remodelers cooperate here, but nonessentially, and none of the many tested single or combined remodeler gene mutations could prevent PHO5 promoter opening. RSC, the most abundant and only remodeler essential for viability, was a controversial candidate for the unrecognized remodeling activity but unassessed in vivo. Now we show that remodels the structure of chromatin (RSC) is crucially involved in PHO5 promoter opening. Further, the isw1 chd1 double deletion also delayed chromatin remodeling. Strikingly, combined absence of RSC and Isw1/Chd1 or Snf2 abolished for the first time promoter opening on otherwise sufficient induction in vivo. Together with previous findings, we recognize now a surprisingly complex network of five remodelers (RSC, SWI/SNF, INO80, Isw1 and Chd1) from four subfamilies (SWI/SNF, INO80, ISWI and CHD) as involved in PHO5 promoter chromatin remodeling. This is likely the first described complete remodeler set for a physiological chromatin transition. RSC was hardly involved at the coregulated PHO8 or PHO84 promoters despite cofactor recruitment by the same transactivator and RSC's presence at all three promoters. Therefore, promoter-specific chromatin rather than transactivators determine remodeler requirements.
Laboratory of Biochemistry, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb 10000, Croatia and Molecular Biology, Adolf-Butenandt-Institut, University of Munich, Munich 80336, Germany.
Sanja
S
Musladin
Nils
N
Krietenstein
Philipp
P
Korber
Slobodan
S
Barbaric
D000135:Acid Phosphatase / Q000096:biosynthesis / Q000235:genetics*; D000251:Adenosine Triphosphatases / Q000235:genetics; D000469:Alkaline Phosphatase / Q000235:genetics; D018797:Cell Cycle Proteins / Q000037:antagonists & inhibitors; D042002:Chromatin Assembly and Disassembly; D016213:Cyclins / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism / Q000502:physiology*
C489847:CHD1 protein, S cerevisiae; D018797:Cell Cycle Proteins; D016213:Cyclins; D004268:DNA-Binding Proteins; C052130:GAL4 protein, S cerevisiae; D009687:Nuclear Proteins; C066824:PHO80 protein, S cerevisiae; C103337:RSC complex, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D000469:Alkaline Phosphatase; C510355:PHO8 protein, S cerevisiae; D000135:Acid Phosphatase; C488916:PHO5 protein, S cerevisiae; D000251:Adenosine Triphosphatases; C470546:ISW1 protein, S cerevisiae; C077392:STH1 protein, S cerevisiae