Human plasma stability during handling and storage: impact on NMR metabolomics.
This work contributes to fill in some existing gaps in the knowledge of human plasma degradability during handling and storage, a paramount issue in Nuclear Magnetic Resonance (NMR) metabolomics. Regarding the comparison between heparin and EDTA anti-coagulant collection tubes, the former showed no interference of the polysaccharide, while conserving full spectral information. In relation to time/temperature conditions, room temperature was seen to have a large impact on lipoproteins and choline compounds from 2.5 hours. In addition, short-term storage at -20 °C was found suitable up to 7 days but, for periods up to 1 month, -80 °C was recommended. Furthermore, in the case of reusing plasma samples, no more than 3 consecutive freeze-thaw cycles were found advisable. Finally, the impact of long-term -80 °C storage (up to 2.5 years) was found almost negligible, as evaluated on a partially matched non-fasting cohort (n = 49), after having investigated the possible confounding nature of the particular non-fasting conditions employed.
CICECO - Department of Chemistry, Campus Universitário de Santiago, Universidade de Aveiro, 3810-193 Aveiro, Portugal. agil@ua.pt.
Joana
J
Pinto
M Rosário M
MR
Domingues
Eulália
E
Galhano
Cristina
C
Pita
Maria do Céu
Mdo C
Almeida
Isabel M
IM
Carreira
Ana M
AM
Gil
D000328:Adult; D001793:Blood Preservation / Q000379:methods / Q000592:standards*; D005260:Female; D006801:Humans; D055432:Metabolomics / Q000379:methods / Q000592:standards*; D008875:Middle Aged; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D055550:Protein Stability; D013048:Specimen Handling / Q000379:methods / Q000592:standards*; D055815:Young Adult