Expression and prognostic relevance of centromere protein A in primary osteosarcoma.
Centromere protein A (CENP-A) is one of the fundamental components of the human active kinetochore and plays important roles in cell-cycle regulation, cell survival, and genetic stability. The aim of the present study was to explore the expression and prognostic significance of CENP-A in osteosarcoma. The results of real-time quantitative PCR and Western blotting analysis revealed an enhanced expression of CENP-A in osteosarcomas relative to adjacent non-tumorous bone tissues at both mRNA and protein levels. Immunohistochemically, 72 of the 123 osteosarcoma specimens (58.5%) had high expression of CENP-A. CENP-A overexpression was significantly correlated with tumor size (P=0.002), poor response to neoadjuvant chemotherapy (P=0.016), local recurrence/lung metastasis (P=0.001), high Ki-67 index (P=0.004), and P53 positivity (P=0.005). Median overall and recurrence-free survival time was significantly shorter in patients with high-CENP-A osteosarcomas than in those with low-CENP-A osteosarcomas. Multivariate analysis identified CENP-A as an independent poor prognostic factor for osteosarcoma. In conclusion, our results demonstrate that elevated CENP-A expression is significantly associated with osteosarcoma progression and has an independent prognostic value in predicting overall and recurrence-free survival for patients with osteosarcoma.
Department of Orthopedics, Affiliated Hospital of Hangzhou Normal University, Hangzhou, PR China. Electronic address: xmgu1971@163.com.
Xiao-Min
XM
Gu
Department of Radiation Oncology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai, PR China.
Jie
J
Fu
Department of Pathology, Yueyang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, PR China.
Xiao-Jun
XJ
Feng
Department of Pathology, Yueyang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, PR China.
Xue
X
Huang
Department of Pathology, Yueyang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, PR China.
Shou-Mei
SM
Wang
Department of Pathology, Changhai Hospital and Institute of Liver Diseases, Second Military Medical University, Shanghai, PR China.
Xin-Feng
XF
Chen
Department of Pathology, Changhai Hospital and Institute of Liver Diseases, Second Military Medical University, Shanghai, PR China.
Ming-Hua
MH
Zhu
Department of Pathology, Yueyang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, PR China.
Shu-Hui
SH
Zhang
D000293:Adolescent; D000328:Adult; D001324:Autoantigens / Q000032:analysis / Q000096:biosynthesis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D001859:Bone Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis / Q000096:biosynthesis*; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D012516:Osteosarcoma / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D011379:Prognosis; D016016:Proportional Hazards Models; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046888:Tissue Array Analysis; D055815:Young Adult
D001324:Autoantigens; D014408:Biomarkers, Tumor; C000619700:CENPA protein, human; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone
Centromere protein A; Immunohistochemistry; Osteosarcoma; Prognostic factor