microRNA-146 up-regulation predicts the prognosis of non-small cell lung cancer by miRNA in situ hybridization.
Non-small cell lung cancer (NSCLC) accounts for approximately 70% of all lung cancer-related deaths worldwide. Prognostic markers are essential for the early detection of lung cancer in patients. In this study, we first identified microRNA146 (miR-146) expression in cancer cell lines using miRNA in situ hybridization (MISH) and confirmed the accuracy of MISH using q-RT-PCR. In addition, two different systems, BCIP/NBT and ELF, were used to detect the signal for a comparative analysis of the specificity of MISH. Compared to the BCIP/NBT system, the ELF detection system was more effective for MISH. Furthermore we detected the expression of miR-146 in NSCLC tissues (43 cases) and normal tissues (32 cases). Based on our results, we can conclude that miR-146 is more highly expressed in cancer tissue than normal tissue (t-test, P<0.05) and that miR-146 can predict the prognosis of NSCLC by MISH. Our findings preliminary demonstrate that MISH can be applied as a molecular diagnostic tool to determine the expression and localization of miRNAs in cancer tissues and that miR-146, determined by MISH, predicts the prognosis of NSCLC patients.
Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Jiangchao
J
Li
Department of Thoracic Surgery, Sun Yat-Sen University Cancer Center, Guangzhou, China.
Hong
H
Yang
State Key Laboratory of Oncology in Southern China, Sun Yat-Sen University Cancer Center, Guangzhou, China.
Yan
Y
Li
Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Ying
Y
Liu
State Key Laboratory of Oncology in Southern China, Sun Yat-Sen University Cancer Center, Guangzhou, China.
Shupeng
S
Chen
Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Cuiling
C
Qi
Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Qianqian
Q
Zhang
Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Tian
T
Lan
Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Xiaodong
X
He
State Key Laboratory of Oncology in Southern China, Sun Yat-Sen University Cancer Center, Guangzhou, China. Electronic address: xyguan@hku.hk.
Xin-Yuan
XY
Guan
Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou 510006, China. Electronic address: wanglijing@gdpu.edu.cn.
Lijing
L
Wang
D014408:Biomarkers, Tumor / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017403:In Situ Hybridization; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D011379:Prognosis
D014408:Biomarkers, Tumor; C542542:MIRN146 microRNA, human; D035683:MicroRNAs
ISH; NSCLC; Prognosis; microRNA-146