Chromosome Y centromere array deletion leads to impaired centromere function.
The centromere is an essential chromosomal structure that is required for the faithful distribution of replicated chromosomes to daughter cells. Defects in the centromere can compromise the stability of chromosomes resulting in segregation errors. We have characterised the centromeric structure of the spontaneous mutant mouse strain, BALB/cWt, which exhibits a high rate of Y chromosome instability. The Y centromere DNA array shows a de novo interstitial deletion and a reduction in the level of the foundation centromere protein, CENP-A, when compared to the non-deleted centromere array in the progenitor strain. These results suggest there is a lower threshold limit of centromere size that ensures full kinetochore function during cell division.
Murdoch Childrens Research Institute, Melbourne, Victoria, Australia.
Alison N
AN
Graham
Murdoch Childrens Research Institute, Melbourne, Victoria, Australia ; Department of Paediatrics, University of Melbourne, Melbourne, Victoria, Australia.
Paul
P
Kalitsis
D000818:Animals; D001324:Autoantigens / Q000235:genetics* / Q000378:metabolism; D015139:Blotting, Southern; D002455:Cell Division / Q000235:genetics / Q000502:physiology; D002503:Centromere / Q000235:genetics* / Q000502:physiology; D000076247:Centromere Protein A; D043171:Chromosomal Instability / Q000235:genetics*; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D017404:In Situ Hybridization, Fluorescence; D018386:Kinetochores / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008954:Models, Biological; D017384:Sequence Deletion / Q000235:genetics*; D014998:Y Chromosome / Q000235:genetics*
D001324:Autoantigens; C000619701:Cenpa protein, mouse; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone; D017931:DNA Primers