Effects of compound Shenhua tablet on renal tubular Na+-K+-ATPase in rats with acute ischemic reperfusion injury.
OBJECTIVE
To observe the effect of Compound Shenhua Tablet (, SHT) on the sodium-potassium- exchanging adenosinetriphosphatase (Na(+)-K(+)-ATPase) in the renal tubular epithelial cells of rats with acute ischemic reperfusion and to investigate the mechanisms underlying the effects of SHT on renal ischemic reperfusion injury (RIRI).


METHODS
Fifty male Wistar rats were randomly divided into the sham surgery group, model group, astragaloside group [150 mg/(kg·d)], SHT low-dose group [1.5 g/(kg·d)] and SHT high-dose group [3.0 g/(kg·d)], with 10 rats in each group. After 1 week of continuous intragastric drug administration, surgery was performed to establish the model. At either 24 or 72 h after the surgery, 5 rats in each group were sacrificed, blood biochemistry, renal pathology, immunoblot and immunohistochemical examinations were performed, and double immunofluorescence staining was observed under a laser confocal microscope.


RESULTS
Compared with the sham surgery group, the serum creatinine (SCr) and blood urea nitrogen (BUN) levels were significantly increased, Na(+)-K(+)-ATPase protein level was decreased, and kidney injury molecule-1 (KIM-1) protein level was increased in the model group after the surgery (P<0.01 or P<0.05). Compared with the model group, the SCr, BUN, pathological scores, Na(+)-K(+)-ATPase, and the KIM-1 protein level of the three treatment groups were significantly improved at 72 h after the surgery (P<0.05 or P<0.01). And the SCr, BUN of the SHT low- and high-dose groups, and the pathological scores of the SHT high-dose group were significantly lower than those of the astragaloside group (P<0.05). The localizations of Na(+)-K(+)-ATPase and megalin of the model group were disrupted, with the distribution areas overlapping with each other and alternately arranged. The severity of the disruption was slightly milder in three treatment groups compared with that of the model group. The results of immunofluorescence staining showed that the SHT high-dose group had a superior effect as compared with the astragaloside group and the SHT low-dose group.


CONCLUSIONS
The SHT effectively alleviated RIRI caused by ischemic reperfusion, promoted the recovery of the polarity of renal tubular epithelial cells, and protected the renal tubules. The therapeutic effects of SHT were superior to those of astragaloside as a single agent.
State Discipline and State Key Laboratory of Kidney Disease (Chinese PLA General Hospital, 2011DAV00088), Beijing, 100853, China.
Yue
Y
Yang
Ri-bao
RB
Wei
Xiao-yong
XY
Zheng
Qiang
Q
Qiu
Shao-yuan
SY
Cui
Zhong
Z
Yin
Suo-zhu
SZ
Shi
Xiang-mei
XM
Chen
D000208:Acute Disease; D000818:Animals; D001806:Blood Urea Nitrogen; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002853:Chromatography, Liquid; D003404:Creatinine / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D007677:Kidney Function Tests; D007684:Kidney Tubules / Q000098:blood supply* / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology; D026561:Low Density Lipoprotein Receptor-Related Protein-2 / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology*; D012503:Saponins / Q000032:analysis; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013194:Staining and Labeling; D013607:Tablets
D015815:Cell Adhesion Molecules; D003404:Creatinine; D004365:Drugs, Chinese Herbal; D026561:Low Density Lipoprotein Receptor-Related Protein-2; D012503:Saponins; D000254:Sodium-Potassium-Exchanging ATPase; D013607:Tablets; C110936:Havcr1 protein, rat; C000592784:shenhua