Laser speckle contrast imaging with extended depth of field for in-vivo tissue imaging.
This work presents, to our knowledge, the first demonstration of the Laser Speckle Contrast Imaging (LSCI) technique with extended depth of field (DOF). We employ wavefront coding on the detected beam to gain quantitative information on flow speeds through a DOF extended two-fold compared to the traditional system. We characterize the system in-vitro using controlled microfluidic experiments, and apply it in-vivo to imaging the somatosensory cortex of a rat, showing improved ability to image flow in a larger number of vessels simultaneously.
The Edward S. Rogers Sr. Department of Electrical and Computer Engineering, 10 King's College Road, Toronto, ON M5S 3G4, Canada ; Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON M5S 3G9, Canada.
Iliya
I
Sigal
The Edward S. Rogers Sr. Department of Electrical and Computer Engineering, 10 King's College Road, Toronto, ON M5S 3G4, Canada ; Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON M5S 3G9, Canada.
Raanan
R
Gad
Department of Electrical, Computer, and Energy Engineering, University of Colorado at Boulder, Boulder, UCB 425, CO 80309, USA.
Antonio M
AM
Caravaca-Aguirre
The Edward S. Rogers Sr. Department of Electrical and Computer Engineering, 10 King's College Road, Toronto, ON M5S 3G4, Canada ; Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON M5S 3G9, Canada.
Yaaseen
Y
Atchia
Department of Electrical, Computer, and Energy Engineering, University of Colorado at Boulder, Boulder, UCB 425, CO 80309, USA.
Donald B
DB
Conkey
Department of Electrical, Computer, and Energy Engineering, University of Colorado at Boulder, Boulder, UCB 425, CO 80309, USA.
Rafael
R
Piestun
The Edward S. Rogers Sr. Department of Electrical and Computer Engineering, 10 King's College Road, Toronto, ON M5S 3G4, Canada ; Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON M5S 3G9, Canada.
Ofer
O
Levi
(110.6150) Speckle imaging; (110.7348) Wavefront encoding; (170.6480) Spectroscopy, speckle