Role of omega-3 polyunsaturated fatty acids in the production of prostaglandin E2 and nitric oxide during experimental murine paracoccidioidomycosis.
There has recently been increased interest in the potential health effects of omega-3 polyunsaturated fatty acids on the immune system. Paracoccidioidomycosis is the most important endemic mycosis in Latin America. Macrophages have a fundamental role and act as first line of organism defense. The purpose of this study was to analyze the effect of n-3 fatty acids on the production of PGE2 and NO by mice infected with Pb18 and fed a diet enriched with LNA for 8 weeks. To study the effect of omega-3 fatty acids on macrophage activity during experimental paracoccidioidomycosis, mice were infected with Pb18 and fed a diet supplemented with LNA. PGE2 in the serum of animals was analyzed and NO in the supernatants of macrophages cultured and challenged in vitro with Pb18 was measured. Omega-3 fatty acids seemed to decrease the production of PGE2 in vivo in the infected group fed an LNA-supplemented diet during the 4th and 8th weeks of the experiment. At the same time, we observed an increase in synthesis of NO by peritoneal macrophages in this group. Omega-3 fatty acids thus appear to have an immunomodulatory effect in paracoccidioidomycosis.
Department of Agricultural Sciences-Food Science, State University of Maringa, Avenida Colombo 5.790, 87.020-900 Maringá, PR, Brazil.
S C
SC
Sargi
Department of Basic Health Sciences, State University of Maringa, Avenida Colombo 5.790, 87.020-900 Maringá, PR, Brazil.
M M O
MM
Dalalio
Department of Basic Health Sciences, State University of Maringa, Avenida Colombo 5.790, 87.020-900 Maringá, PR, Brazil.
A G
AG
Moraes
Department of Basic Health Sciences, State University of Maringa, Avenida Colombo 5.790, 87.020-900 Maringá, PR, Brazil.
J E L
JE
Visentainer
Department of Chemistry, State University of Maringa, Avenida Colombo 5.790, 87.020-900 Maringá, PR, Brazil.
D R
DR
Morais
Department of Chemistry, State University of Maringa, Avenida Colombo 5.790, 87.020-900 Maringá, PR, Brazil.
J V
JV
Visentainer
D000818:Animals; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage; D015232:Dinoprostone / Q000096:biosynthesis* / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000378:metabolism*; D006801:Humans; D007113:Immunity, Innate; D008264:Macrophages / Q000276:immunology / Q000382:microbiology; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis*; D010228:Paracoccidioides / Q000378:metabolism / Q000472:pathogenicity*; D010229:Paracoccidioidomycosis / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology
D004042:Dietary Fats, Unsaturated; D015525:Fatty Acids, Omega-3; D009569:Nitric Oxide; D015232:Dinoprostone