Molecular cloning, expression, and regulation of the ovalbumin gene in pigeon oviduct epithelial cells.
The full-length pigeon ovalbumin (OVA) gene cDNA was cloned and sequenced by reverse transcription-polymerase chain reaction (RT-PCR) and rapid-amplification of cDNA ends. A 386-amino acid protein was predicted for the obtained sequence, which had 67% identity with the chicken protein. Similar to chicken OVA, the pigeon OVA gene is a non-inhibitory serine protease inhibitor. Quantitative PCR analysis revealed that pigeon OVA mRNA was highly expressed in the oviduct, and trace amounts were detected in other tissues. During the reproductive cycle, pigeon oviduct OVA mRNA expression reached its peak during the egg-laying stage, decreased with brooding, and then increased again during the squab-feeding period. Moreover, the relative OVA expression level in pigeon oviduct epithelial cells could be upregulated by a constant concentration of steroid hormones.
College of Animal Sciences and Technology, Nanjing Agriculture University, Nanjing, Jiangsu, China.
H
H
Zhang
Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China.
L Z
LZ
Lu
Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China.
L
L
Chen
Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China.
Z R
ZR
Tao
College of Animal Sciences and Technology, Nanjing Agriculture University, Nanjing, Jiangsu, China.
F
F
Chen
PingYang XingLiang Pigeon Farming Co. Ltd., Wenzhou, Zhejiang, China.
S L
SL
Zhong
Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China.
Y L
YL
Liu
Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China.
Y
Y
Tian
College of Animal Sciences and Technology, Nanjing Agriculture University, Nanjing, Jiangsu, China yanps@njau.edu.cn.
P S
PS
Yan
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D010856:Columbidae / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D004847:Epithelial Cells / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D012739:Gonadal Steroid Hormones / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010047:Ovalbumin / Q000235:genetics* / Q000378:metabolism; D010057:Oviducts / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D012739:Gonadal Steroid Hormones; D012333:RNA, Messenger; D010047:Ovalbumin