A procedure for the in vivo determination of enzyme activity in higher plant tissue.
Rapid freezing of higher plant tissue in liquid nitrogen renders the cells permeable to a wide range of substrate molecules. Tissue permeabilized by repeated freeze-thaw treatment can be used for the measurement of several enzymes. With most of the tissues examined maximum in vivo activities were obtained using a combination of freeze-thaw treatment followed by vacuum infiltration. The activities and properties of enzymes determined with this procedure are very comparable with those obtained for enzymes prepared by conventional extraction procedures.
Department of Botany, The University, M13 9PL, Manchester, UK.
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Stewart