[Recombinant thrombopoietin antiradiation therapeutic effectiveness evaluation on dogs according to hemopoiesis and survival criteria].
Recombinant human thrombopoietin (rh TPO) has been investigated as a means of acute radiation disease urgent treatment in the experiments on 24 mongrel dogs. The animals were exposed to total acute gamma-irradiation at the doses of 3.5 Gy (exceeding LD50/45 under our conditions) and 3 Gy. All the dogs including control ones received antibiotics Ampicillin and Gentamicin twice a day during the acute period from the 7th to the 21st day. TPO was injected one time s/c or i/v at the doses of5 or 10 mkg/kg 1.5-2 h after exposure. TPO at a dose of 5 mkg/kg was ineffective. TPO at a dose of 10 mkg/kg had a positive effect on the kinetics of blood forming units, especially platelets (nadir, restoration rate) in terms of the 45-day survival. As a result, in TPO groups, nadir averaged at both exposure doses on leucocytes (1.3-1.4) x 10(9)/l vs (0.70-0.75) x 10(9)/l in control groups and on thrombocytes (102-112) x 10(9)/l vs (44-33) x 10(9)/l in control ones. Despite the low number of animals in experimental groups, the results permit to regard rhTPO as a worth-while urgent therapeutic means for the acute radiation damage treatment and preventing thrombopenia.
L M
LM
Rozhdestvenskiĭ
T G
TG
Shliakova
R A
RA
Shchegoleva
N I
NI
Lisina
V V
VV
Zorin
D054508:Acute Radiation Syndrome / Q000188:drug therapy* / Q000473:pathology; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004285:Dogs; D005720:Gamma Rays; D006410:Hematopoiesis / Q000187:drug effects* / Q000528:radiation effects; D006801:Humans; D007700:Kinetics; D011837:Radiation-Protective Agents / Q000008:administration & dosage; D011994:Recombinant Proteins / Q000008:administration & dosage*; D013926:Thrombopoietin / Q000008:administration & dosage*; D014916:Whole-Body Irradiation
D011837:Radiation-Protective Agents; D011994:Recombinant Proteins; D013926:Thrombopoietin