Calorie restriction dose-dependently abates lipopolysaccharide-induced fever, sickness behavior, and circulating interleukin-6 while increasing corticosterone.
In mice a 50% calorie restriction (CR) for 28days attenuates sickness behavior after lipopolysaccharide (LPS) and these mice demonstrate a central anti-inflammatory bias. This study examined the dose-dependent effect of CR on sickness behavior (fever, anorexia, cachexia) and peripheral immune markers post-LPS. Male Sprague-Dawley rats fed ad libitum or CR by 50% for 14, 21, or 28days were injected on day 15, 22, or 29 with 50μg/kg of LPS or saline (1mL/500g). Changes in body temperature (Tb), locomotor activity, body weight, and food intake were determined. A separate cohort of rats was fed ad libitum or CR by 50% for 28days and serum levels of corticosterone (CORT), interleukin 6 (IL-6), and IL-10 were determined at 0, 2, and 4h post-LPS. The rats CR for 28days demonstrated the largest attenuation of sickness behavior: no fever, limited reduction in locomotor activity, no anorexia, and reduced cachexia following LPS. Rats CR for 14 and 21days demonstrated a partial attenuation of sickness behavior. Rats CR for 14days demonstrated a larger increase in Tb, larger reduction in locomotor activity, and larger weight loss compared to rats CR for 21days. Serum CORT was increased at 2h post-LPS in ad libitum and CR groups; however it was two times larger in the CR animals. Levels of IL-6 were significantly attenuated at 2h post-LPS in the CR animals. IL-10 levels were similar post-LPS. CR results in an enhanced anti-inflammatory response in the form of increased CORT and diminished pro-inflammatory signals.
School of Psychological Science, La Trobe University, Melbourne, VIC 3086, Australia.
Leah
L
MacDonald
School of Psychological Science, La Trobe University, Melbourne, VIC 3086, Australia.
Agnes
A
Hazi
School of Psychological Science, La Trobe University, Melbourne, VIC 3086, Australia.
Antonio G
AG
Paolini
School of Psychological Science, La Trobe University, Melbourne, VIC 3086, Australia. Electronic address: S.Kent@latrobe.edu.au.
Stephen
S
Kent
D000818:Animals; D001835:Body Weight; D031204:Caloric Restriction; D003345:Corticosterone / Q000097:blood*; D004435:Eating; D005334:Fever / Q000178:diet therapy*; D055809:Illness Behavior; D007249:Inflammation; D016753:Interleukin-10 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood*; D008070:Lipopolysaccharides; D008297:Male; D009043:Motor Activity; D051381:Rats; D017207:Rats, Sprague-Dawley
D015850:Interleukin-6; D008070:Lipopolysaccharides; D016753:Interleukin-10; D003345:Corticosterone
Corticosterone; Cytokines; Dietary restriction; Fever; Food restriction; Interleukin-10; Interleukin-6; Lipopolysaccharide; Sickness behavior