[Expression of E-cadherin and beta1-integrin mRNA in endometrial cancer].
OBJECTIVE
The metastatic ability of tumors is characteristic for malignant neoplasms and constitutes the main cause of therapeutics failures. Metastasis formation involves the sequence of processes such as proteolytic degradation of the basement membrane, migration, intravasation, extravasation, proliferation and angiogenesis. Cadherins and integrins are groups of proteins directly involved in these processes. In the present study we analyzed the mRNA expression of CDH1 and ITGB1 genes by real-time polymerase chain reaction (RT-PCR). The study included 106 endometrial carcinomas. CDH1 and ITGB1 mRNA expression was found in all of the studied samples. Generally the CDH1 and ITGB1 mRNA expression was significantly higher in well-differentiated rather than poorly differentiated tumors.


METHODS
The mRNA expression levels of CDH1 and ITGB1 in series of 83 samples of endometrial carcinoma were studied by real time RT-PCR method. Statistical analysis of the obtained results was performed.


RESULTS
CDH1 and ITGB1 gene expression was observed in all examined tissues and was correlated with cancer malignancy (G). In high grade malignant tumors (G1), CDH1 and ITGB1 gene expression was the highest, in G2 and G3 tumors the expression of both genes was gradually lowering. Moreover the statistically significant correlation between CDH1 and ITGB1 gene expression was observed. (Spearman test, r=0.29, p<O.01).


CONCLUSIONS
CDH1 and ITGB1 mRNA expression seems to be an important marker of cancer progression and metastases in endometrial malignant tumors.
Department of Gynecological Oncology, Medical University of Łódź, Poland. katarzyna.wojcik-krowiranda@umed.lodz.pl
Katarzyna
K
Wójcik-Krowiranda
Department of Cytobiochemistry, University of Łódź, Poland.
Ewa
E
Forma
Department of Cytobiochemistry, University of Łódź, Poland.
Agnieszka
A
Zaczek
Department of Cytobiochemistry, University of Łódź, Poland.
Magdalena
M
Bryś
Department of Cytobiochemistry, University of Łódź, Poland.
Magdalena Krześlak
MK
Anna
Department of Gynecological Oncology, Medical University of Łódź, Poland.
Andrzej
A
Bieńkiewicz
D015703:Antigens, CD; D015820:Cadherins / Q000235:genetics*; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D019012:Integrin beta1 / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; D019012:Integrin beta1; D012333:RNA, Messenger