Comments on "innovative method for carbon dioxide determination in human postmortem cardiac gas samples using headspace-gas chromatography-mass spectrometry and stable labeled isotope as internal standard" by Varlet et al.
Varlet et al. recently proposed a headspace-gas chromatography-mass spectrometry (HS-GC-MS) method applicable for the routine determination of CO2 in gaseous biologic matrices. This developed bioanalytical method was fully validated according to the SFSTP 1997 guidelines using the accuracy profile as a graphical decision-making tool.In this letter, we discuss the validity of HS-GC-MS method based on the newest SFSTP guideline. In particular, we demonstrate by the estimation of the β-expectation tolerance interval that the error total exceeds the acceptance limits (30%) for the second concentration level (0.5μmol mL(-1) vial HS). Furthermore, we show through the risk profile that the probability to have future results inside the ±30% acceptance limits is smaller than 95%.
Centre Universitaire Régional d'Interface, Université Sidi Mohamed Ben Abdellah, BP 2626, route d'Imouzzer, Fes Morocco; Laboratoire de Chimie Organique Appliquée, Faculté des Sciences et Techniques, BP 2202, route d'Imouzzer, Fes Morocco. Electronic address: taoufiq.saffaj@usmba.ac.ma.
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Saffaj
Laboratoire de Chimie Organique Appliquée, Faculté des Sciences et Techniques, BP 2202, route d'Imouzzer, Fes Morocco.
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Ihssane
D001013:Aorta, Thoracic / Q000737:chemistry*; D002245:Carbon Dioxide / Q000032:analysis*; D049429:Forensic Pathology / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006325:Heart Atria / Q000737:chemistry*; D006801:Humans
D002245:Carbon Dioxide
Accuracy profile; Analytical validation; SFSTP 1997; SFSTP 2003; Tolerance interval