Spectral demultiplexing in holographic and fluorescent on-chip microscopy.
Lensfree on-chip imaging and sensing platforms provide compact and cost-effective designs for various telemedicine and lab-on-a-chip applications. In this work, we demonstrate computational solutions for some of the challenges associated with (i) the use of broadband, partially-coherent illumination sources for on-chip holographic imaging, and (ii) multicolor detection for lensfree fluorescent on-chip microscopy. Specifically, we introduce spectral demultiplexing approaches that aim to digitally narrow the spectral content of broadband illumination sources (such as wide-band light emitting diodes or even sunlight) to improve spatial resolution in holographic on-chip microscopy. We also demonstrate the application of such spectral demultiplexing approaches for wide-field imaging of multicolor fluorescent objects on a chip. These computational approaches can be used to replace e.g., thin-film interference filters, gratings or other optical components used for spectral multiplexing/demultiplexing, which can form a desirable solution for cost-effective and compact wide-field microscopy and sensing needs on a chip.
Electrical Engineering Department, University of California Los Angeles, Los Angeles, California, United States of America.
Ikbal
I
Sencan
Electrical Engineering Department, University of California Los Angeles, Los Angeles, California, United States of America.
Ahmet F
AF
Coskun
Electrical Engineering Department, University of California Los Angeles, Los Angeles, California, United States of America.
Uzair
U
Sikora
1] Electrical Engineering Department, University of California Los Angeles, Los Angeles, California, United States of America [2] Bioengineering Department, University of California Los Angeles, Los Angeles, California, United States of America [3] California NanoSystems Institute, University of California Los Angeles, Los Angeles, California, United States of America.
Aydogan
A
Ozcan
D003952:Diagnostic Imaging; D006696:Holography; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008856:Microscopy, Fluorescence; D013472:Sunlight; D017216:Telemedicine