Characterization of a synthetic bioactive polymer by nonlinear optical microscopy.
Tissue Engineering is a new emerging field that offers many possibilities to produce three-dimensional and functional tissues like ligaments or scaffolds. The biocompatibility of these materials is crucial in tissue engineering, since they should be integrated in situ and should induce a good cell adhesion and proliferation. One of the most promising materials used for tissue engineering are polyesters such as Poly-ε-caprolactone (PCL), which is used in this work. In our case, the bio-integration is reached by grafting a bioactive polymer (pNaSS) on a PCL surface. Using nonlinear microscopy, PCL structure is visualized by SHG and proteins and cells by two-photon excitation autofluorescence generation. A comparative study between grafted and nongrafted polymer films is provided. We demonstrate that the polymer grafting improves the protein adsorption by a factor of 75% and increase the cell spreading onto the polymer surface. Since the spreading is directly related to cell adhesion and proliferation, we demonstrate that the pNaSS grafting promotes PCL biocompatibility.
Université Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, CNRS (UMR 7244), 74 rue Marcel Cachin, 93017, Bobigny, France.
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N
Djaker
Institut Fresnel, MOSAIC, CNRS, Aix-Marseille Université, Ecole Centrale Marseille, Domaine Universitaire St Jérôme, France.
S
S
Brustlein
Université Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, CNRS (UMR 7244), 99 avenue JB Clément, 93430, Villetaneuse, France.
G
G
Rohman
Université Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, CNRS (UMR 7244), 99 avenue JB Clément, 93430, Villetaneuse, France.
S
S
Huot
Université Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, CNRS (UMR 7244), 74 rue Marcel Cachin, 93017, Bobigny, France.
M Lamy
ML
de la Chapelle
Université Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, CNRS (UMR 7244), 99 avenue JB Clément, 93430, Villetaneuse, France.
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V
Migonney
(160.1435) Biomaterials; (180.4315) Nonlinear microscopy