Tissue engineered esophagus by copper--small intestinal submucosa graft for esophageal repair in a canine model.
Acellular porcine small intestinal submucosa (SIS) has been used for esophagoplasty with success in a canine model. However, it did not lead to complete epithelialization. For better reconstruction, a cellular component is required. Moreover, promotion of angiogenesis with copper has been widely recognized by basic research as well as clinical studies. In this study, we have evaluated the feasibility and effectiveness of combined Cu and SIS (SIS-Cu patch) for the esophageal repair using a canine model. Eighteen male beagle dogs were subjected to surgical resection to produce cervical esophageal defects (5 cm in length, 180° in range). SIS with Cu (5 or 25 μmol L(-1) copper) or without Cu was patched on the esophageal defects. Barium esophagram and histology exam were carried out to evaluate the effectiveness of the therapy. As shown, the SIS-Cu graft promoted re-epithelialization, re-vascularization and muscular regeneration. SIS-Cu patch is more effective than SIS alone for esophageal repair, and the SIS+25 μmol L(-1) Cu group demonstrated additional advantages over the SIS+5 μmol L(-1) Cu.
Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu, 610041, China.
Bo
B
Tan
Min
M
Wang
Xi
X
Chen
JiangLong
J
Hou
XiaoHe
X
Chen
Yue
Y
Wang
Jesse
J
Li-Ling
HuiQi
H
Xie
D000818:Animals; D004285:Dogs; D004947:Esophagus / Q000601:surgery*; D007150:Immunohistochemistry; D007421:Intestine, Small / Q000637:transplantation*; D008297:Male; D023421:Models, Animal; D013552:Swine; D023822:Tissue Engineering