Effect of deltamethrin (pyrethroid insecticide) on two clones of Daphnia magna (Crustacea, Cladocera): a proteomic investigation.
Deltamethrin is a class II pyrethroid insecticide commonly used in agriculture. It is hazardous to freshwater ecosystems, especially for the cladoceran Daphnia magna (Straus 1820). The results of our previous studies based on acute and chronic ecotoxicity experiments revealed differences in the sensitivity between two different clones. In this work, to investigate deltamethrin toxicity mechanisms in two clones of D. magna, we used a proteomic approach in order to analyze changes in protein expression profiles after 48 h of exposure. We detected 1339 spots; then applying statistical criteria (ANOVA p<0.001 and minimum fold change 1.5), only 128 spots were significantly different in the normalized volume. Among the preselected proteins there were 88 up-regulated and 40 down-regulated proteins. Results showed differences in sensitivities after deltamethrin exposure between the clones. Moreover, using the 2-DIGE method, proteomic investigation for deltamethrin exposure proved to be a reliable and powerful approach to investigate effects of deltamethrin as part of research for new metabolic and cellular biomarkers. After identification by mass spectrometry, there were 39 proteins recognized and identified, in which 21 and 18 were up- and down-regulated, respectively, in deltamethrin-exposed clone A compared to three other conditions (controls of each clone and deltamethrin-exposed clone 2). Up- and down-regulated proteins belonged to 12 biological processes (i.e. metabolic processes, apoptosis and stimulus response) and 5 molecular functions (i.e. catalytic activity, binding, structural molecular activity, antioxidant and receptor activities). Identification of these deregulated proteins opens a new way in discovering new molecular targets and putative biomarkers in daphnids exposed to deltamethrin.
Laboratoire de Bio-surveillance de l'Environnement (LBE), Unité d'Hydrobiologie littorale et limnique, Université de Carthage, Faculté des Sciences de Bizerte, 7021 Zarzouna, Bizerte, Tunisia; Université de Lorraine, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), UMR 7360 CNRS, Campus Bridoux, Bât. IBiSE, Rue du Général Delestraint, 57070 Metz, France.
Héla
H
Toumi
Laboratoire de Bio-surveillance de l'Environnement (LBE), Unité d'Hydrobiologie littorale et limnique, Université de Carthage, Faculté des Sciences de Bizerte, 7021 Zarzouna, Bizerte, Tunisia.
Moncef
M
Boumaiza
Université de Bourgogne, Laboratoire BIOGEOSCIENCES, UMR 6282 CNRS, UFR Sciences Vie Terre et Environnement, 6 boulevard Gabriel, 21000 Dijon, France.
Françoise
F
Immel
Université de Lorraine, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), UMR 7360 CNRS, Campus Bridoux, Bât. IBiSE, Rue du Général Delestraint, 57070 Metz, France.
Bénédicte
B
Sohm
Université de Lorraine, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), UMR 7360 CNRS, Campus Bridoux, Bât. IBiSE, Rue du Général Delestraint, 57070 Metz, France.
Vincent
V
Felten
Université de Lorraine, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), UMR 7360 CNRS, Campus Bridoux, Bât. IBiSE, Rue du Général Delestraint, 57070 Metz, France. Electronic address: jean-francois.ferard@univ-lorraine.fr.
Jean-François
JF
Férard
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002999:Clone Cells / Q000187:drug effects; D003621:Daphnia / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects*; D009570:Nitriles / Q000633:toxicity*; D040901:Proteomics; D011722:Pyrethrins / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D015415:Biomarkers; D009570:Nitriles; D011722:Pyrethrins; D014874:Water Pollutants, Chemical; C017180:decamethrin
Clones; Daphnia magna; Deltamethrin; Proteomic