Rapid detection of Enterococcus spp. direct from blood culture bottles using Enterococcus QuickFISH method: a multicenter investigation.
The performance of a diagnostic method for detection and identification of Enterococcus spp. directly from positive blood culture was evaluated in a clinical study. The method, Enterococcus QuickFISH BC, is a second-generation peptide nucleic acid (PNA) fluorescence in situ hybridization (FISH) test, which uses a simplified, faster assay procedure. The test uses fluorescently labeled PNA probes targeting 16S rRNA to differentiate Enterococcus faecalis from other Enterococcus spp. by the color of the cellular fluorescence. Three hundred fifty-six routine blood culture samples were tested; only 2 discordant results were recorded. The sensitivities for detection of Enterococcus faecalis and non-faecalis Enterococcus were 100% (106/106) and 97.0% (65/67), respectively, and the combined specificity of the assay was 100%. The combined positive and negative predictive values of the assay were 100% (171/171) and 98.9% (185/187), respectively.
AdvanDx Inc., Woburn, MA. Electronic address: mkd@advandx.com.
Melissa K
MK
Deck
AdvanDx Inc., Woburn, MA.
Erica S
ES
Anderson
Indiana University School of Medicine, Indianapolis, IN.
Rebecca J
RJ
Buckner
Health Network Laboratories, Allentown, PA.
Georgia
G
Colasante
Indiana University School of Medicine, Indianapolis, IN.
Thomas E
TE
Davis
AdvanDx Inc., Woburn, MA.
James M
JM
Coull
AdvanDx Inc., Woburn, MA.
Benjamin
B
Crystal
Columbia University Medical Center, NewYork-Presbyterian Medical Center, New York, NY.
Phyllis Della
PD
Latta
AdvanDx Inc., Woburn, MA.
Martin
M
Fuchs
Indiana University School of Medicine, Indianapolis, IN.
Deanna
D
Fuller
AdvanDx Inc., Woburn, MA.
Will
W
Harris
Duke University, Durham, NC.
Kevin
K
Hazen
AdvanDx Inc., Woburn, MA.
Lisa L
LL
Klimas
Health Network Laboratories, Allentown, PA.
Daniel
D
Lindao
AdvanDx Inc., Woburn, MA.
Michelle C
MC
Meltzer
Cedars-Sinai Medical Center, Los Angeles, CA.
Margie
M
Morgan
AdvanDx Inc., Woburn, MA.
Janeen
J
Shepard
AdvanDx Inc., Woburn, MA.
Sharon
S
Stevens
Columbia University Medical Center, NewYork-Presbyterian Medical Center, New York, NY.
Fann
F
Wu
AdvanDx Inc., Woburn, MA.
Mark J
MJ
Fiandaca
D001769:Blood / Q000382:microbiology*; D016983:Enterococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D018805:Sepsis / Q000175:diagnosis* / Q000382:microbiology
Enterococcus; PNA; QuickFISH; septicemia