Decomposition of size-exclusion chromatography elution curves of complex branched polymers.
A new method for the decomposition of non-baseline-resolved multimodal elution curves of SEC with the concentration, light scattering and viscosity detection is presented. The method makes possible the characterization of the polymer-sample components, represented by the peaks forming multimodal elution curves, individually and reduces also the error in the calculation of molecular-weight averages. The procedure is demonstrated on narrow molecular-weight distribution polystyrene standards and their mixture as well as on a grafted polymer sample.
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovský Square 2, 162 06 Prague 6, Czech Republic. Electronic address: netopilik@imc.cas.cz.
Miloš
M
Netopilík
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovský Square 2, 162 06 Prague 6, Czech Republic.
Miroslav
M
Janata
D002850:Chromatography, Gel / Q000379:methods* / Q000592:standards; D008027:Light; D008970:Molecular Weight; D011137:Polystyrenes / Q000032:analysis*; D012015:Reference Standards; D012542:Scattering, Radiation; D014783:Viscosity
D011137:Polystyrenes
Curve decomposition; Size-exclusion chromatography; Triple detection