Development of a composite chiral stationary phase from BSA and β-cyclodextrin-bonded silica.
A composite chiral stationary phase (CSP) derived from bovine serum albumin (BSA) and β-cyclodextrin (CD)-bonded silica was prepared. 2,4,6-Trichloro-1,3,5-triazine was used as a cross-linker. The obtained CSP was applied to the enantioseparation of tryptophan, hydrobenzoin, phenylalanine and mandelic acid. The influences of eluent pH value, organic modifier and column temperature on the retention and enantioseparation were discussed. Tryptophan and hydrobenzoin achieved excellent resolution on the composite CSP. For tryptophan, the highest selectivity, 2.79, was achieved with 1% of methanol at pH 8.0. For hydrobenzoin, the selectivity could reach 1.42. The chromatographic results were compared with that on β-CD-bonded or BSA-immobilized CSP.
Department of Chemistry and Environmental Science, Minnan Normal University, 36 Xianqian Straight Street, Xiangcheng District, Zhangzhou 363000, China Key Laboratory for Analytical Science of Fujian Province University, Zhangzhou 363000, China.
Bixia
B
Yao
Department of Chemistry and Environmental Science, Minnan Normal University, 36 Xianqian Straight Street, Xiangcheng District, Zhangzhou 363000, China Key Laboratory for Analytical Science of Fujian Province University, Zhangzhou 363000, China.
Xinmei
X
Yang
Department of Chemistry and Environmental Science, Minnan Normal University, 36 Xianqian Straight Street, Xiangcheng District, Zhangzhou 363000, China Key Laboratory for Analytical Science of Fujian Province University, Zhangzhou 363000, China.
Lizhen
L
Guo
Department of Chemistry and Environmental Science, Minnan Normal University, 36 Xianqian Straight Street, Xiangcheng District, Zhangzhou 363000, China Key Laboratory for Analytical Science of Fujian Province University, Zhangzhou 363000, China.
Shanshan
S
Kang
Department of Chemistry and Environmental Science, Minnan Normal University, 36 Xianqian Straight Street, Xiangcheng District, Zhangzhou 363000, China Key Laboratory for Analytical Science of Fujian Province University, Zhangzhou 363000, China weng_wen@126.com.
Wen
W
Weng
D000818:Animals; D001573:Benzoin / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification; D002417:Cattle; D002853:Chromatography, Liquid / Q000295:instrumentation*; D006863:Hydrogen-Ion Concentration; D008333:Mandelic Acids / Q000737:chemistry / Q000302:isolation & purification; D008956:Models, Chemical; D010649:Phenylalanine / Q000737:chemistry / Q000302:isolation & purification; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013237:Stereoisomerism; D013816:Thermodynamics; D014227:Triazines / Q000737:chemistry; D014364:Tryptophan / Q000737:chemistry / Q000302:isolation & purification; D047392:beta-Cyclodextrins / Q000737:chemistry*
D008333:Mandelic Acids; D014227:Triazines; D047392:beta-Cyclodextrins; C540375:hydrobenzoin; D012710:Serum Albumin, Bovine; D010649:Phenylalanine; D012822:Silicon Dioxide; D014364:Tryptophan; D001573:Benzoin; C037938:mandelic acid