[Effects of arbuscular mycorrhizal fungi on the vegetation restoration of different types of coal mine spoil banks].
A greenhouse pot experiment was conducted to investigate the influence of arbuscular mycorrhizal (AM) fungi Glomus etunicatum (GE) and Glomus versiforme (GV) on the plant growth, nutrient uptake, C: N: P stoichiometric, uptake of heavy metals by maize (Zea mays L.) grown in three types of coal mine spoil banks. The aim was to provide a technical basis for the revegetation of coal mine spoil banks in grassland ecosystem. The results indicated that the symbiotic associations were successfully established between two isolates and maize grown in the three substrates, with an average mycorrhizal colonization rate ranging from 36% to 54%. The colonization of two AM fungi significantly increased the dry weight of maize grown in recent discharged and weathered coal mine spoils and GE increased those grown in weathered coal mine spoil. Inoculation with AM fungi promoted the uptake of N, P and K by maize to varying degrees. In addition, inoculation with GE and GV also decreased C: N: P ratios, supporting the growth rate hypothesis, and had significantly differences on concentrations of Cu, Fe, Mn and Zn in shoots and roots of maize. The results indicated that GE and GV had different mycorrhizal effects on maize in the three types of substrates. GV was more suitable for the revegetation of recent discharged coal mine spoil and weathered coal mine spoil, while GE was more suitable for the revegetation of spontaneous combusted coal mine spoil. The experiment demonstrates that AM fungi have a potential role for maize to enhance the ability to adapt the composite adversity of different types of coal mine spoil and play a positive role in the revegetation of different coal mine spoil banks. Further field experiments should be conducted to evaluate the practical effects of AM fungi on the vegetation restoration of different types of coal mine spoil under field conditions.
College of Environment and Resources of Inner Mongolia University, Hohhot 010021, China. renxinzhao09@163.com
Ren-Xin
RX
Zhao
College of Environment and Resources of Inner Mongolia University, Hohhot 010021, China.
Wei
W
Guo
College of Environment and Resources of Inner Mongolia University, Hohhot 010021, China.
Rui-Ying
RY
Fu
College of Environment and Resources of Inner Mongolia University, Hohhot 010021, China.
Wen-Jing
WJ
Zhao
College of Environment and Resources of Inner Mongolia University, Hohhot 010021, China.
Jiang-Yuan
JY
Guo
College of Environment and Resources of Inner Mongolia University, Hohhot 010021, China.
Na
N
Bi
College of Chemistry and Chemical Engineering of Inner Mongolia University, Hohhot 010021, China.
Jun
J
Zhang
D003031:Coal; D017753:Ecosystem; D052918:Environmental Restoration and Remediation; D019216:Metals, Heavy / Q000032:analysis; D008906:Mining; D038821:Mycorrhizae / Q000502:physiology*; D018517:Plant Roots / Q000382:microbiology; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis; D003313:Zea mays / Q000254:growth & development / Q000382:microbiology*
D003031:Coal; D019216:Metals, Heavy; D012989:Soil Pollutants