Proteome and allergenome of Asian wasp, Vespa affinis, venom and IgE reactivity of the venom components.
Vespa affinis (Asian wasp, Thai banded tiger wasp, or local name: Tor Hua Seua) causes the most frequent incidence of medically important Hymenoptera sting in South and Southeast Asia. However, data on the venom components attributable to the sting derived-clinical manifestations (local reactions, IgE mediated-anaphylaxis, or systemic envenomation) are lacking. This study provides the first set information on V. affinis venom proteome, allergenome, and IgE reactivity of individual venom components. From 2DE-gel based-proteomics, the venom revealed 93 protein spots, of which proteins in 51 spots could be identified and classified into three groups: typical venom components and structural and housekeeping proteins. Venom proteins in 32 spots reacted with serum IgE of wasp allergic patients. Major allergenic proteins that reacted to IgE of >50% of the wasp allergic patients included PLA1 (100%), arginine kinase (73%), heat shock 70 kDa protein (73.3%), venom allergen-5 (66.7%), enolase (66.7%), PLA1 magnifin (60%), glyceraldehyde-3-phosphate dehydrogenase (60%), hyaluronidase (53.3%), and fructose-bisphosphate aldolase (53.3%). The venom minor allergens were GB17876 transcript (40%), GB17291 transcript (20%), malic enzyme (13.3%), aconitate hydratase (6.7%), and phosphoglucomutase (6.7%). The information has diagnostic and clinical implications for future improvement of case diagnostic sensitivity and specificity, component-resolve diagnosis, and design of specific Hymenoptera venom immunotherapy.
Department of Research and Development, ‡Graduate Program in Immunology, Department of Immunology, §Department of Parasitology, and ∥Department of Pediatrics, Faculty of Medicine Siriraj Hospital, Mahidol University , Bangkok 10700, Thailand.
Nitat
N
Sookrung
Siriporn
S
Wong-din-Dam
Anchalee
A
Tungtrongchitr
Onrapak
O
Reamtong
Nitaya
N
Indrawattana
Yuwaporn
Y
Sakolvaree
Nualanong
N
Visitsunthorn
Wiparat
W
Manuyakorn
Wanpen
W
Chaicumpa
D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology / Q000302:isolation & purification*; D000707:Anaphylaxis / Q000097:blood / Q000276:immunology* / Q000503:physiopathology; D000818:Animals; D002648:Child; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood*; D007299:Insect Bites and Stings / Q000097:blood / Q000276:immunology* / Q000503:physiopathology; D019476:Insect Proteins / Q000276:immunology / Q000302:isolation & purification*; D008297:Male; D058977:Molecular Sequence Annotation; D011485:Protein Binding; D020543:Proteome / Q000276:immunology / Q000302:isolation & purification*; D014862:Wasp Venoms / Q000737:chemistry*; D014863:Wasps / Q000502:physiology
D000485:Allergens; D019476:Insect Proteins; D020543:Proteome; D014862:Wasp Venoms; D007073:Immunoglobulin E