Tunable aqueous polymer-phase impregnated resins-technology-a novel approach to aqueous two-phase extraction.
Aqueous Two-Phase Extraction (ATPE) represents a promising unit operation for downstream processing of biotechnological products. The technique provides several advantages such as a biocompatible environment for the extraction of sensitive and biologically active compounds. However, the tendency of some aqueous two-phase systems to form intensive and stable emulsions can lead to long phase separation times causing an increased footprint for the required mixer-settler devices or the need for additional equipment such as centrifuges. In this work, a novel approach to improve ATPE for downstream processing applications called 'Tunable Aqueous Polymer-Phase Impregnated Resins' (TAPPIR(®))-Technology is presented. The technology is based on the immobilization of one aqueous phase inside the pores of a solid support. The second aqueous phase forms the bulk liquid around the impregnated solids. Due to the immobilization of one phase, phase emulsification and phase separation of ATPE are realized in a single step. In this study, a biodegradable and sustainable aqueous two-phase system consisting of aqueous polyethylene glycol/sodiumcitrate solutions was chosen. The impregnation of different macroporous glass and ceramic solids was investigated and could be proven to be stable. Additionally, the separation of the dye Patent blue V was successfully performed with the TAPPIR(®)-Technology. Thus, the "proof of principle" of this technology is presented.
Laboratory for Plant and Process Design, Department of Biochemical and Chemical Engineering, Technische Universität Dortmund, D-44227 Dortmund, Germany.
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FA
van Winssen
Laboratory for Plant and Process Design, Department of Biochemical and Chemical Engineering, Technische Universität Dortmund, D-44227 Dortmund, Germany. Electronic address: juliane.merz@bci.tu-dortmund.de.
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J
Merz
Laboratory for Plant and Process Design, Department of Biochemical and Chemical Engineering, Technische Universität Dortmund, D-44227 Dortmund, Germany.
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G
Schembecker
D001709:Biotechnology; D007700:Kinetics; D059625:Liquid-Liquid Extraction / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D014867:Water; D011092:Polyethylene Glycols
Aqueous two-phase extraction; Downstream processing; Dye partitioning; Phase immobilization; TAPPIR(®); Tunable aqueous polymer-phase impregnated resins