Endometrial inflammation and abnormal expression of extracellular matrix proteins induced by Mycoplasma bovis in dairy cows.
Mycoplasma bovis infection can cause endometrial inflammation leading to infertility and involuntary culling in dairy cows. Because extracellular matrix (ECM) proteins affect the adherence of mycoplasma to eukaryotic cell surface, they may play a role in the pathogenesis of the bacteria. The objective of the present study was to evaluate the endometrial inflammatory response and ECM protein expression induced by M bovis. Endometrial concentrations of inflammatory cytokines tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and mRNA and protein expression of collagen IV (CL-IV), fibronectin (FN), and laminin (LN) were evaluated 10, 20, and 30 days after M bovis intrauterine infusion in breed cows 18 days postpartum. The presence of the bacteria in the uterus was detected by nested polymerase chain reaction and denaturing gradient gel electrophoresis. Endometrial TNF-α, IL-1β, and IL-6 concentrations in the treatment group were greater (P < 0.05) than in the positive and negative control groups 20 and 30 days after infusion. Endometrial CL-IV, FN, and LN mRNA and protein expression increased (P < 0.01) 20 days after infusion in all groups. However, the increase was more pronounced in the treatment group and reactive expressions were greater (P < 0.05) than in the positive and negative control groups 10, 20, and 30 days after infusion. In conclusion, M bovis triggered endometrial inflammatory response and increased CL-IV, FN, and LN mRNA and protein expression. The abnormal expression of ECM these proteins may promote the pathogenic effects of M bovis that lead to endometrial tissue damage and infertility.
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, China.
Mengyao
M
Guo
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, China; Institute of Biosciences and Biotechnology, Northeastern University, Shenyang, China.
Guoqing
G
Wang
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, China.
Tingting
T
Lv
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, China.
Xiaojing
X
Song
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, China.
Tiancheng
T
Wang
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, China.
Guanghong
G
Xie
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, China.
Yongguo
Y
Cao
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, China. Electronic address: naisheng.zhang@wur.nl.
Naisheng
N
Zhang
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, China; College of Animal Science and Veterinary medicine, Qingdao Agricultural University, Qingdao, Shandong, China. Electronic address: qing0001@163.com.
Rongfeng
R
Cao
D000818:Animals; D002417:Cattle; D024141:Collagen Type IV / Q000032:analysis / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D004716:Endometritis / Q000378:metabolism / Q000382:microbiology / Q000662:veterinary*; D004717:Endometrium / Q000737:chemistry / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000032:analysis / Q000235:genetics*; D005260:Female; D005353:Fibronectins / Q000032:analysis / Q000235:genetics; D015870:Gene Expression; D007247:Infertility, Female / Q000382:microbiology / Q000662:veterinary; D053583:Interleukin-1beta / Q000032:analysis; D015850:Interleukin-6 / Q000032:analysis; D007797:Laminin / Q000032:analysis / Q000235:genetics; D009175:Mycoplasma Infections / Q000150:complications / Q000662:veterinary*; D045682:Mycoplasma bovis / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D014599:Uterus / Q000382:microbiology
D024141:Collagen Type IV; D004269:DNA, Bacterial; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D053583:Interleukin-1beta; D015850:Interleukin-6; D007797:Laminin; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha
Dairy cow; Endometrial inflammation; Extracellular matrix; Mycoplasma bovis