Poly(amidoamine) dendrimer-enabled simultaneous stabilization and functionalization of electrospun poly(γ-glutamic acid) nanofibers.
We report a facile and general approach to using generation 2 (G2) poly(amidoamine) (PAMAM) dendrimers for simultaneous stabilization and functionalization of electrospun poly(γ-glutamic acid) nanofibers (γ-PGA NFs). In this study, uniform γ-PGA NFs with a smooth morphology were generated using electrospinning technology. In order to endow the NFs with good water stability, amine-terminated G2.NH2 PAMAM dendrimers were utilized to crosslink the γ-PGA NFs via 1-ethyl-3-(3-dimethylami-nopropyl) carbodiimide coupling chemistry. Under the optimized crosslinking conditions, G2.NH2 dendrimers partially modified with fluorescein isothiocyanate (FI) or folic acid (FA) were used to crosslink γ-PGA NFs. Our results reveal that G2.NH2-FI is able to simultaneously render the NFs with good water stability and fluorescence property, while G2.NH2-FA is able to simultaneously endow the NFs with water stability and the ability to capture FA receptor-overexpressing cancer cells in vitro via ligand-receptor interaction. With the tunable dendrimer surface chemistry, multifunctional water-stable γ-PGA-based NFs may be generated via a dendrimer crosslinking approach, thereby providing diverse applications in the areas of biosensing, tissue engineering, drug delivery, and environmental sciences.
State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University , Shanghai 201620, People's Republic of China.
Shige
S
Wang
Jingyi
J
Zhu
Mingwu
M
Shen
Meifang
M
Zhu
Xiangyang
X
Shi
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D003432:Cross-Linking Reagents / Q000737:chemistry; D050091:Dendrimers / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing / Q000379:methods*; D051379:Mice; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010078:Oxazines / Q000378:metabolism; D010316:Particle Size; D011073:Polyamines / Q000737:chemistry*; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D066244:Proton Magnetic Resonance Spectroscopy; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D014966:Xanthenes / Q000378:metabolism
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D050091:Dendrimers; D010078:Oxazines; C104700:PAMAM Starburst; C531249:Poly(amidoamine); D011073:Polyamines; D014966:Xanthenes; C511775:poly(gamma-glutamic acid); C005843:resazurin; D011099:Polyglutamic Acid