CTL induction by DNA vaccine with Toxoplasma gondii-HSP70 gene.
Toxoplasma gondii-derived heat shock protein 70 (T.g.HSP70) is a tachyzoite-specific virulent molecule. The DNA vaccine with T.g.HSP70 gene targeting peripheral epidermal or dermal dendritic cells (DC) induces in vivo DC maturation and successive early Th1 polarization at the draining lymph nodes (dLN) of C57BL/6 mice. In the present study, induction of cytotoxic T lymphocytes (CTL) has been explored. The CTL specific for a syngeneic DC line, DC2.4, either transfected with T. g.HSP70 gene or pulsed with recombinant T. g.HSP70 are induced in the spleen of the vaccinated mice. This CTL lyses T. gondii-infected, but not uninfected, DC2.4. Both CD8(+) and CD4(+) CTL are induced by the vaccine, and Fas/Fas ligand-mediated cytolysis dominantly participates in their CTL activities. Adoptive transfer experiments reveal that the vaccine-induced CD8(+) or CD4(+) T cells possess a protective role for toxoplasmosis at both acute and chronic phases of infection.
Department of Infection and Host Defense, Chiba University Graduate School of Medicine, 1-8-1 Inohana Chuo-ku, Chiba 260-8670, Japan.
Deyong
D
Chu
Department of Infection and Host Defense, Chiba University Graduate School of Medicine, 1-8-1 Inohana Chuo-ku, Chiba 260-8670, Japan.
Masataka
M
Moroda
Department of Infection and Host Defense, Chiba University Graduate School of Medicine, 1-8-1 Inohana Chuo-ku, Chiba 260-8670, Japan.
Lian-Xun
LX
Piao
Department of Infection and Host Defense, Chiba University Graduate School of Medicine, 1-8-1 Inohana Chuo-ku, Chiba 260-8670, Japan. Electronic address: aosai@faculty.chiba-u.jp.
Fumie
F
Aosai
D000818:Animals; D015496:CD4-Positive T-Lymphocytes; D018414:CD8-Positive T-Lymphocytes; D002460:Cell Line; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016052:Protozoan Vaccines / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000502:physiology*; D014122:Toxoplasma / Q000276:immunology / Q000378:metabolism*; D014124:Toxoplasmosis, Animal / Q000517:prevention & control*; D019444:Vaccines, DNA / Q000276:immunology
D018840:HSP70 Heat-Shock Proteins; D016052:Protozoan Vaccines; D019444:Vaccines, DNA
Cytotoxic T lymphocytes (CTL); DC vaccine; T. gondii HSP70; Toxoplasma gondii