Tributyltin contributes in reducing the vascular reactivity to phenylephrine in isolated aortic rings from female rats.
Organotin compounds such as tributyltin (TBT) are used as antifouling paints by shipping companies. TBT inhibits the aromatase responsible for the transformation of testosterone into estrogen. Our hypothesis is that TBT modulates the vascular reactivity of female rats. Female Wistar rats were treated daily (Control; CONT) or TBT (100 ng/kg) for 15 days. Rings from thoracic aortas were incubated with phenylephrine (PHE, 10(-10)-10(-4) M) in the presence and absence of endothelium, and in the presence of N(G)-Nitro-L-Arginine Methyl Ester (L-NAME), tetraethylammonium (TEA) and apocynin. TBT decreased plasma levels of estrogen and the vascular response to PHE. In the TBT group, the vascular reactivity was increased in the absence of endothelium, L-NAME and TEA. The decrease in PHE reactivity during incubation with apocynin was more evident in the TBT group. The sensitivity to acetylcholine (ACh) and sodium nitroprusside (SNP) was reduced in the TBT group. TBT increased collagen, reduced α1-smooth muscle actin. Female rats treated with TBT for 15 days showed morphology alteration of the aorta and decreased their vascular reactivity, probably due to mechanisms dependent on nitric oxide (NO) bioavailability, K(+) channels and an increase in oxidative stress.
Department of Physiological Sciences, Federal University of Espirito Santo, Vitoria, Espírito Santo, Brazil. Electronic address: samyalimaro@hotmail.com.
Samya Mere L
SM
Rodrigues
Department of Physiological Sciences, Federal University of Espirito Santo, Vitoria, Espírito Santo, Brazil.
Carolina F
CF
Ximenes
Department of Physiological Sciences, Federal University of Espirito Santo, Vitoria, Espírito Santo, Brazil.
Priscila R
PR
de Batista
Department of Physiological Sciences, Federal University of Espirito Santo, Vitoria, Espírito Santo, Brazil.
Fabiana V
FV
Simões
Department of Morphology, Federal University of Espirito Santo-UFES, Espírito Santo, Brazil.
Pedro Henrique P
PH
Coser
Department of Morphology, Federal University of Espirito Santo-UFES, Espírito Santo, Brazil.
Gabriela C
GC
Sena
Department of Morphology, Federal University of Espirito Santo-UFES, Espírito Santo, Brazil.
Priscila L
PL
Podratz
Department of Morphology, Federal University of Espirito Santo-UFES, Espírito Santo, Brazil.
Leticia N G
LN
de Souza
Department of Physiological Sciences, Federal University of Espirito Santo, Vitoria, Espírito Santo, Brazil; Department of Physiology, EMESCAM, Espírito Santo, Brazil.
Dalton V
DV
Vassallo
Department of Morphology, Federal University of Espirito Santo-UFES, Espírito Santo, Brazil. Electronic address: jbgraceli@ccs.ufes.br.
Jones B
JB
Graceli
Department of Physiological Sciences, Federal University of Espirito Santo, Vitoria, Espírito Santo, Brazil. Electronic address: ivanitaufes@hotmail.com.
Ivanita
I
Stefanon
D000098:Acetophenones / Q000494:pharmacology; D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D004347:Drug Interactions; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D004958:Estradiol / Q000378:metabolism; D005260:Female; D006651:Histocytochemistry; D015151:Immunoblotting; D016014:Linear Models; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D010656:Phenylephrine / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D019789:Tetraethylammonium / Q000494:pharmacology; D014220:Trialkyltin Compounds / Q000633:toxicity*; D014661:Vasoconstriction / Q000187:drug effects
D000098:Acetophenones; D014220:Trialkyltin Compounds; D009599:Nitroprusside; D010656:Phenylephrine; D004958:Estradiol; C011559:tributyltin; D019789:Tetraethylammonium; C056165:acetovanillone; D000109:Acetylcholine; D019331:NG-Nitroarginine Methyl Ester
Aorta rings.; Estrogen; PHE; Tributyltin; Vascular reactivity