[Relationship between anthocyanin synthesis and activity of phenylalanine ammonia-lyase (PAL) in callus cells of Daucus carota].
Two celllines from Daucus carota callus were tested for phenylalanine ammonia-lyase activity. The white callus (DCw) has only 15% of the enzyme activity of the blue callus (DCb). The enzyme activity reaches a maximum value after a cultivation period of 21 days, whereas the maximum in anthocyanin content is reached after 28 days. We can demonstrate here a good correlation between one of the enzymes and the end product of the anthocyanin biosynthesis.The crude extract from DCw cells has no inhibitory effect on the enzyme from DCb-cells. Gibberellic acid A3, a component of the culture medium for DCw cells, does not act as an inhibitor on the partially purified enzyme. The procedure of purification and the characteristics of phenylalanine ammonia-lyase from the callus of Daucus carota are described.
Institut für Biologie der Universität, Auf der Morgenstelle 1, D-7400, Tübingen, Federal Republic of Germany.
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Heinzmann
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Seitz