Human spermatozoa cryopreservation: comparison of three different freezing protocols.
Human spermatozoa cryopreservation has significantly improved over the last few decades, but the actual protocols are neither optimal nor standardized between different laboratories in spite of the importance of preserving male fertility or treating severely infertile males. In the present study we aimed to determine the best in-house method of rapid freezing in terms of sperm motility and vitality by comparing three different rapid methods of human spermatozoa cryopreservation. Our data showed that M1 (triphasic cooling) is the method associated with a significantly lower deterioration of semen quality in comparison with mono or biphasic cooling. Differences observed among the three protocols were supported by statistical analysis. These data reinforce previous evidences about the influence of sperm quality on IVF outcome and suggest the importance of improving sperm cryopreservation techniques especially when semen is seriously compromised at baseline.
Research Center for Reproductive Medicine, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy. claudia.omes@unipv.it
Claudia
C
Omes
Scientific Direction, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
Anna Lisa
AL
Marchetti
Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Italy.
Maria Luisa
ML
Masanti
Research Center for Reproductive Medicine, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
Roberto
R
Bassani
Clinical Epidemiology and Biometric Unit, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
Carmine
C
Tinelli
Research Center for Reproductive Medicine, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
Rossella Chiara
RC
Sanarica
Research Center for Reproductive Medicine, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
Arsenio
A
Spinillo
Research Center for Reproductive Medicine, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
Rossella Elena
RE
Nappi
D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods*; D006801:Humans; D008297:Male; D055101:Semen Analysis; D012662:Semen Preservation / Q000379:methods*; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology*