Interaction between nucleophosmin and HBV core protein increases HBV capsid assembly.
Host factors are involved in Hepatitis B virus (HBV) genome replication and capsid formation during the viral life cycle. A host factor, nucleophosmin (B23), was found to bind to HBV core protein dimers, but its functional role has not been studied. This interaction promoted HBV capsid assembly and decreased the degree of capsid dissociation when subjected to denaturant treatments in vitro. In addition, inhibition of B23 reduced intracellular capsid formation resulting in a decrease of HBV production in HepG2.2.15 cells. These results provide important evidence that B23 acts on core capsid assembly via its interaction with HBV core dimers.
Department of Biological Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
Heewon
H
Jeong
Department of Biological Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
Min-Hyung
MH
Cho
School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea.
Sung-Gyoo
SG
Park
Department of Biological Sciences, Seoul National University, Seoul 151-742, Republic of Korea. Electronic address: drjung@snu.ac.kr.
Guhung
G
Jung
D001665:Binding Sites; D036022:Capsid Proteins / Q000737:chemistry / Q000378:metabolism*; D004279:DNA, Viral / Q000235:genetics; D056945:Hep G2 Cells; D006515:Hepatitis B virus / Q000502:physiology*; D006801:Humans; D008958:Models, Molecular; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism*; D000090243:Nucleophosmin; D054730:Protein Interaction Domains and Motifs; D055503:Protein Multimerization; D055550:Protein Stability; D014758:Viral Core Proteins / Q000737:chemistry / Q000378:metabolism*; D019065:Virus Assembly; D014779:Virus Replication
D036022:Capsid Proteins; D004279:DNA, Viral; C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D014758:Viral Core Proteins; D000090243:Nucleophosmin
Core assembly; Core protein truncated at residue 149 (Cp149); Encapsidation; Hepatitis B virus (HBV); Host factor; Nucleophosmin (B23)