EA-D p45-IgG as a potential biomarker for nasopharyngeal carcinoma diagnosis.
OBJECTIVE
To identify new biomarkers for NPC diagnosis with an anti-EBV Western blot test kit.


METHODS
Serum samples from 64 NPC patients and healthy subjects with four specific VCA-IgA/EA-IgA profiles were tested with an anti-EBV Western blot test kit from EUROIMMUN AG. Proteins were quantified with scores of intensity visually assigned to the protein bands. The markers which showed statistical differences between the NPC and non-NPC subjects were further evaluated in another 32 NPC patients and 32 controls in comparison with established biomarkers including VCA-IgA, EA-IgA, EBV-related protein IgG, and EBV DNA.


RESULTS
Among the markers screened, EA-D p45-IgG showed a statistically significant difference (p < 0.05) between NPC and non-NPC subjects with VCA-IgA positivy. In 32 VCA-IgA positive NPC patients and 32 control subjects, the diagnostic accuracy of EA-D p45-IgG was 78.1% with a positive predictive value of 77.8% and a negative predictive value of 78.6%. In the verification experiment, the specificity and sensitivity of EA-D p45-IgG were 75.0% and 90.6 %, respectively.


CONCLUSIONS
EA-D p45-IgG might be a potential biomarker for NPC diagnosis, especially among VCA-IgA positive subjects.
Department of Clinical Laboratory, Sun Yat-sen University Cancer Center; State Key Laboratory of Oncology in South China; Collaborative Innovation Center for Cancer Medicine, China E-mail : liuwl @sysucc.org.cn.
Hao
H
Chen
Yao-Ling
YL
Luo
Lin
L
Zhang
Li-Zhen
LZ
Tian
Zhi-Ting
ZT
Feng
Wan-Li
WL
Liu
D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000097:blood*; D036022:Capsid Proteins / Q000276:immunology; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005500:Follow-Up Studies; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D009367:Neoplasm Staging; D011379:Prognosis; D012372:ROC Curve
D000914:Antibodies, Viral; D000956:Antigens, Viral; D014408:Biomarkers, Tumor; D036022:Capsid Proteins; D007074:Immunoglobulin G