Mechanism of inhibition on L929 rat fibroblasts proliferation induced by potential adhesion barrier material poly(p-dioxanone-co-L-phenylalanine) electrospun membranes.
Fibroblast plays an important role in the occurrence of postoperative tissue adhesion; materials that have particular "cell-material" interactions to inhibit proliferation of fibroblast will be excellent potential adhesion barriers. In the current study, we synthesized copolymers of p-dioxanone and L-phenylalanine (PDPA) and evaluated the mechanism of its particular inhibition effect on L929 fibroblast proliferation when used as a culture surface. PDPA electrospun membranes could induce apoptosis of L929 fibroblasts. We hypothesized there were two reasons for the apoptosis induction: one was the ability to facilitate cell adhesion of materials, and the other was production of the degradation product, L-phenylalanine. Ninhydrin colorimetric results revealed that L-phenylalanine was continuously released during the culture process and could induce apoptosis in L929 cells. Relatively poor cell adhesion and constant release of L-phenylalanine made PDPA-1 to be the most efficient polymer for the induction of apoptosis. Analysis of apoptosis-related genes revealed that PDPA-induced apoptosis might be performed in a mitochondrial-dependent pathway. But poly(p-dioxanone)-induced apoptosis might occur in a c-Myc independent pathway that was different from PDPA.
Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu, China; Department of Polymer Chemistry and Physics, University of Chinese Academy of Sciences, Beijing, China.
Bing
B
Wang
Jun
J
Dong
Qijie
Q
Li
Zuochun
Z
Xiong
Chengdong
C
Xiong
Dongliang
D
Chen
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D008567:Membranes, Artificial; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D016687:Polydioxanone / Q000737:chemistry / Q000494:pharmacology*; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D051381:Rats
D008567:Membranes, Artificial; D010455:Peptides; D016271:Proto-Oncogene Proteins c-myc; C012001:polyphenylalanine; D016687:Polydioxanone
apoptosis pathway; degradation product release; induce fibroblast apoptosis; poly(p-dioxanone-co-l-phenylalanine); postoperative adhesion barrier