Identification of peptides from soybean protein, glycinin, possessing suppression of intracellular Ca2+ concentration in vascular smooth muscle cells.
In this study, we challenged to identify vasoactive peptides in soybean 11S glycinin hydrolysate by thermolysin to regulate intracellular Ca(2+) concentration ([Ca(2+)]i) that can induce constrictive vascular tension. As a function of the inhibition of elevated [Ca(2+)]i by 10 μM angiotensin (Ang) II in vascular smooth muscle cells (VSMCs), eleven peptides were successfully identified from the hydrolysate, among which His-Gly-Lys exhibited the most potent inhibition against [Ca(2+)]i elevation in Ang II-stimulated VSMCs (inhibition at 300 μM: 46.5±8.0% vs. control). The biological capacity of His-Gly-Lys analogues as an [Ca(2+)]i inhibitor was also proven when His-Lys and His-Gly-Arg elicited a significant reduction in [Ca(2+)]i. In contrast, less reduction of [Ca(2+)]i by His-Gly-Ile and His-(3-methyl)-Gly-Lys indicated the importance of the imino proton in His, along with basic amino acids positioned at C-terminal for the effect.
Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Graduated School of Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
Thanutchaporn
T
Kumrungsee
Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Graduated School of Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
Zheng-Quan
ZQ
Wang
Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Graduated School of Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
Shinya
S
Matsumura
Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Graduated School of Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
Tomomi
T
Saiki
Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Graduated School of Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
Mitsuru
M
Tanaka
Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Graduated School of Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan. Electronic address: tmatsui@agr.kyushu-u.ac.jp.
Toshiro
T
Matsui
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D005916:Globulins / Q000737:chemistry* / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology*; D011492:Protein Hydrolysates / Q000737:chemistry / Q000494:pharmacology; D011921:Rats, Inbred WKY; D030262:Soybean Proteins / Q000737:chemistry* / Q000494:pharmacology*; D013025:Glycine max / Q000737:chemistry*
D005916:Globulins; D010455:Peptides; D011492:Protein Hydrolysates; D030262:Soybean Proteins; C011474:glycinin; D002118:Calcium
Glycinin peptide; Intracellular Ca(2+); Smooth muscle cell