Hydrogen sulfide offers neuroprotection on traumatic brain injury in parallel with reduced apoptosis and autophagy in mice.
Hydrogen sulfide (H2S), a novel gaseous mediator, has been recognized as an important neuromodulator and neuroprotective agent in the central nervous system. The present study was undertaken to study the effects of exogenous H2S on traumatic brain injury (TBI) and the underlying mechanisms. The effects of exogenous H2S on TBI were examined by using measurement of brain edema, behavior assessment, propidium iodide (PI) staining, and Western blotting, respectively. Compared to TBI groups, H2S pretreatment had reduced brain edema, improved motor performance and ameliorated performance in Morris water maze test after TBI. Immunoblotting results showed that H2S pretreatment reversed TBI-induced cleavage of caspase-3 and decline of Bcl-2, suppressed LC3-II, Beclin-1 and Vps34 activation and maintained p62 level in injured cortex and hippocampus post TBI. The results suggest a protective effect and therapeutic potential of H2S in the treatment of brain injury and the protective effect against TBI may be associated with regulating apoptosis and autophagy.
Department of Forensic Science and Laboratory of Brain Injury, Medical College of Soochow University, Suzhou, China ; Department of Forensic Science, Medical College of Nantong University, Nantong, China.
Mingyang
M
Zhang
Department of Obstetrics and Gynecology, North District of Suzhou Municipal Hospital, Suzhou, China.
Haiyan
H
Shan
Department of Forensic Science and Laboratory of Brain Injury, Medical College of Soochow University, Suzhou, China.
Pan
P
Chang
Department of Forensic Science and Laboratory of Brain Injury, Medical College of Soochow University, Suzhou, China.
Tao
T
Wang
Department of Forensic Science and Laboratory of Brain Injury, Medical College of Soochow University, Suzhou, China.
Wenwen
W
Dong
Department of Forensic Science and Laboratory of Brain Injury, Medical College of Soochow University, Suzhou, China.
Xiping
X
Chen
Department of Forensic Science and Laboratory of Brain Injury, Medical College of Soochow University, Suzhou, China.
Luyang
L
Tao
D000704:Analysis of Variance; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001343:Autophagy / Q000187:drug effects*; D015153:Blotting, Western; D001929:Brain Edema / Q000209:etiology / Q000473:pathology; D001930:Brain Injuries / Q000188:drug therapy*; D006862:Hydrogen Sulfide / Q000494:pharmacology* / Q000627:therapeutic use; D018782:Maze Learning / Q000187:drug effects; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D011419:Propidium; D051758:Transcription Factor TFIIH; D014157:Transcription Factors / Q000378:metabolism
C490981:Gtf2h1 protein, mouse; D018696:Neuroprotective Agents; D014157:Transcription Factors; D051758:Transcription Factor TFIIH; D011419:Propidium; D006862:Hydrogen Sulfide