Analysis of susceptibility polymorphisms for nonsyndromic cleft lip with or without cleft palate in the Brazilian population.
BACKGROUND
Although genome-wide association studies have identified several susceptibility loci for nonsyndromic cleft lip with or without cleft palate (NSCL/P) in populations around the world, the role of most loci is unknown in the highly heterogeneous Brazilian population.


METHODS
To determine the association of 7 markers that showed genome-wide significant association in Brazilians with NSCL/P, we conducted a structured association study conditioned upon the individual ancestry proportions to evaluate markers at 1p36 (rs742071), 2p21 (rs7590268), 3p11.1 (rs7632427), 8q21.3 (rs12543318), 13q31.1 (rs8001641), 15q22.2 (rs1873147), and 17q22 (rs227731) in 505 patients with NSCL/P and 594 healthy controls recruited from 2 different geographical regions of Brazil. The polymorphisms were genotyped by TaqMan 5'-exonuclease allelic discrimination assay, and each sample was independently typed for 40 biallelic short insertion/deletion markers to characterize the genomic ancestry.


RESULTS
After Bonferroni correction for multiple tests, significant associations with NSCL/P were observed for rs742071, rs1873147, and rs227731. However, the frequency of the risk alleles varied between the geographical regions, according to the proportions of European and African genomic ancestry. The group enriched by European ancestry showed significant association with rs227731 (p = 0.001), whereas the group with high African ancestry was significantly associated with rs1873147 polymorphism (p = 0.005). The significant association with rs742071 was only detected in the combined sample (p = 0.005).


CONCLUSIONS
The findings of the present study revealed the associations of 1p36 (rs742071), 15q22 (rs1873147), and 17p22 (rs227731) with NSCL/P in the Brazilian population, and further confirmed that the genetic heterogeneity of NSCL/P may be related to the different ethnic background of the affected individuals.
Department of Oral Diagnosis, School of Dentistry, State University of Campinas, Piracicaba, São Paulo, Brazil.
Sibele Nascimento
SN
de Aquino
Ana Camila
AC
Messetti
Ryuchi
R
Hoshi
Andréa
A
Borges
Camila Sane
CS
Viena
Sílvia R A
SR
Reis
Mário Sérgio
MS
Oliveira Swerts
Edgard
E
Graner
Hercílio
H
Martelli-Júnior
Ricardo D
RD
Coletta
D044383:Black People; D001938:Brazil; D016022:Case-Control Studies; D002878:Chromosomes, Human, Pair 1; D002884:Chromosomes, Human, Pair 15; D002886:Chromosomes, Human, Pair 17; D002971:Cleft Lip / Q000208:ethnology / Q000235:genetics* / Q000473:pathology; D002972:Cleft Palate / Q000208:ethnology / Q000235:genetics* / Q000473:pathology; D005787:Gene Frequency; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D055106:Genome-Wide Association Study; D060005:Genotyping Techniques; D006801:Humans; D040582:Inheritance Patterns; D016254:Mutagenesis, Insertional; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D017384:Sequence Deletion; D044465:White People
D005819:Genetic Markers
15q22.2; 17q22; 1p36; nonsyndromic cleft lip with or without palate; polymorphism