TREK2 expressed selectively in IB4-binding C-fiber nociceptors hyperpolarizes their membrane potentials and limits spontaneous pain.
Ongoing/spontaneous pain behavior is associated with ongoing/spontaneous firing (SF) in adult DRG C-fiber nociceptors (Djouhri et al., 2006). Causes of this SF are not understood. We show here that conducting (sometimes called uninjured) C-nociceptors in neuropathic pain models with more hyperpolarized resting membrane potentials (Ems) have lower SF rates. Understanding the control of their Ems may therefore be important for limiting pathological pain. We report that TREK2, a leak K(+) channel, is selectively expressed in IB4 binding rat C-nociceptors. These IB4(+) C-neurons are ∼10 mV more hyperpolarized than IB4(-) C-neurons in vivo (Fang et al., 2006). TREK2 knockdown by siRNA in these neurons in culture depolarized them by ∼10 mV, suggesting that TREK2 is responsible for this ∼10 mV difference. In vivo, more hyperpolarized C-nociceptor Ems were associated with higher cytoplasmic edge-TREK2 expression (edge-TREK2). Edge-TREK2 decreased in C-neurons 7 d after axotomy, and their Ems depolarized by ∼10 mV. This again supports a contribution of TREK2 to their Ems. These relationships between (1) Em and TREK2, (2) SF rate and Em, and (3) spontaneous pain behavior and C-nociceptor SF rate suggested that TREK2 knockdown might increase spontaneous pain. After CFA-induced inflammation, spontaneous foot lifting (a measure of spontaneous pain) was (1) greater in rats with naturally lower TREK2 in ipsilateral small DRG neurons and (2) increased by siRNA-induced TREK2 knockdown in vivo. We conclude that TREK2 hyperpolarizes IB4 binding C-nociceptors and limits pathological spontaneous pain. Similar TREK2 distributions in small DRG neurons of several species suggest that these role(s) of TREK2 may be widespread.
Institute of Histology and Embriology of Mendoza, Mendoza 5500, Argentina, Department of Biomedical Sciences, Faculty of Medicine, King Faisal University, Al-Hassa 11335, Kingdom of Saudi Arabia, and School of Physiology and Pharmacology, University of Bristol, Bristol BS8 1TD, United Kingdom.
Cristian
C
Acosta
Laiche
L
Djouhri
Roger
R
Watkins
Carol
C
Berry
Kirsty
K
Bromage
Sally N
SN
Lawson
D000818:Animals; D015153:Blotting, Western; D004195:Disease Models, Animal; D004594:Electrophysiology; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism / Q000382:microbiology; D055785:Gene Knockdown Techniques; D007150:Immunohistochemistry; D008564:Membrane Potentials / Q000502:physiology*; D009437:Neuralgia / Q000378:metabolism*; D009619:Nociceptors / Q000378:metabolism*; D024683:Potassium Channels, Tandem Pore Domain / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
C503444:Kcnk10 protein, rat; D024683:Potassium Channels, Tandem Pore Domain
DRG; K2P; TREK2; nociceptors; spontaneous firing; spontaneous pain