The inhibitory effects of a new cobalt-based polyoxometalate on the growth of human cancer cells.
A new cobalt-based polyoxometalate, (Himi)2[Bi(2)W2(0)O(66)(OH)(4)Co2(H2O)(6)Na(4) (H2O)14] · 17H2O (imi = iminazole) (BWCN) has been synthesized and structurally characterized. The inhibitory activities against selected human cancer lines were also determined in this study. The cell viability and chemoresistance of BWCN on human colon carcinoma HT-29 cells were assessed by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazoliumbromide), cell morphology changes, a comet assay and western blot analysis. The typical morphologic changes of apoptosis and DNA damage indicated that BWCN could have a distinct proliferation inhibitory effect on cancer cells. BWCN as a chemotherapeutic agent also induced apoptosis on HT-29 cells and showed a significant expression of cleaved-caspase-3. These results suggested that the active site of BWCN is the polymeric anion based on the basic tectonic block {BiW(9)}, and the possible mechanism is related to the interference of DNA synthesis in cancer cells.
Key Laboratory of Synthesis of Functional Materials and Green Catalysis Colleges of Heilongjiang Province, School of Chemistry and Chemical Engineering, Harbin Normal University, Harbin, Heilongjiang 150025, the People's Republic of China. zhou_bai_bin@163.com.
Lu
L
Wang
Kai
K
Yu
Bai-Bin
BB
Zhou
Zhan-Hua
ZH
Su
Song
S
Gao
Li-Li
LL
Chu
Jia-Ren
JR
Liu
D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003035:Cobalt / Q000737:chemistry / Q000494:pharmacology*; D020552:Comet Assay; D004249:DNA Damage; D006801:Humans; D017973:Tungsten Compounds / Q000737:chemistry / Q000494:pharmacology*; D000099294:Polyoxometalates
D000970:Antineoplastic Agents; D053148:Caspase 3; D003035:Cobalt; D017973:Tungsten Compounds; C102296:polyoxometalate I; D000099294:Polyoxometalates