Sneathiella chungangensis sp. nov., isolated from a marine sand, and emended description of the genus Sneathiella.
A Gram-stain-negative, non-spore-forming, motile, strictly aerobic bacterial strain, designated CAU 1294T, was isolated from a sand sample and its taxonomic position was investigated using a polyphasic approach. The strain grew optimally at pH 6.5 and 30 °C and in the presence of 2% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1294T formed a lineage with member of the genus Sneathiella and exhibited similarity to Sneathiella glossodoripedis MKT133T (96.3% similarity) and Sneathiella chinensis LMG 23452T (95.1% similarity). Strain CAU 1294T contained Q-10 as the predominant respiratory quinone. C18:1ω7c, C16:0 and cyclo-C19:0ω8c were the major cellular fatty acids. The polar lipids were composed of phosphatidylethanolamine, phosphatidylmethylethanolamine and two unidentified phospholipids. The DNA G+C content was 56.6 mol%. On the basis of these results, strain CAU 1294T is considered to represent a novel species of the genus Sneathiella, for which the name Sneathiella chungangensis is proposed. The type strain is CAU 1294T (=KCTC 32476T=CECT 8513T). An emended description of the genus Sneathiella is also proposed.
Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
Chatuphon
C
Siamphan
Rice University, Houston, Texas, USA.
Hyungjin
H
Kim
Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, Yuseong-gu, Daejeon, 305-806, Republic of Korea.
Jung-Sook
JS
Lee
Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
Wonyong
W
Kim
D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012822:Silicon Dioxide; D012988:Soil Microbiology; D014451:Ubiquinone / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D012822:Silicon Dioxide; C025204:Ubiquinone Q2