Inhibitory effects of PLAP-1/asporin on periodontal ligament cells.
PLAP-1/asporin is an extracellular matrix protein that is predominantly expressed in the human periodontal ligament (PDL) and has an aspartic acid (D) repeat polymorphism in its N-terminal region. In this study, we hypothesized that the D repeat polymorphism of PLAP-1/asporin may affect the physiological functions of periodontal ligaments. We established periodontal ligament cell lines transfected with the D13- or D14-PLAP-1 gene. Alkaline phosphatase staining and alizarin red staining revealed that the cytodifferentiation of the D14-PLAP-1-expressing PDL cells was more repressed compared with that of the D13-PLAP-1-expressing cells. Furthermore, the D14-PLAP-1-expressing cells inhibited BMP-2-induced cytodifferentiation more strongly than did the D13-PLAP-1-expressing cells. Western blotting analysis and luciferase assay revealed that D14-PLAP-1 suppressed BMP-2 signal transduction more efficiently than did D13-PLAP-1, and co-immunoprecipitation demonstrated the stronger affinity of the D14-PLAP-1 protein to BMP-2 compared with the D13-PLAP-1 protein. Analysis of these data suggests that the D repeat polymorphism of PLAP-1/asporin has a significant influence on the functions of PDL cells.
Department of Periodontology, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.
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Kajikawa
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Yamada
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Tauchi
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Awata
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Yamaba
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Fujihara
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Murakami
D000469:Alkaline Phosphatase / Q000187:drug effects / Q000235:genetics; D000880:Anthraquinones; D001224:Aspartic Acid / Q000235:genetics; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology; D002113:Calcification, Physiologic / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D004396:Coloring Agents; D017077:Culture Media, Conditioned; D016326:Extracellular Matrix Proteins / Q000235:genetics*; D057809:HEK293 Cells; D006801:Humans; D051798:Inhibitor of Differentiation Protein 1 / Q000032:analysis; D010513:Periodontal Ligament / Q000166:cytology / Q000378:metabolism*; D010957:Plasmids; D011110:Polymorphism, Genetic / Q000235:genetics; D020449:Repetitive Sequences, Amino Acid / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D020079:Terminal Repeat Sequences / Q000235:genetics; D014162:Transfection
C439889:ASPN protein, human; D000880:Anthraquinones; C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D004396:Coloring Agents; D017077:Culture Media, Conditioned; D016326:Extracellular Matrix Proteins; C497453:ID1 protein, human; D051798:Inhibitor of Differentiation Protein 1; D001224:Aspartic Acid; C010078:alizarin; D000469:Alkaline Phosphatase
BMP-2; asporin; cytodifferentiation; extracellular matrix; gene polymorphism; tissue homeostasis