Chromosomal polymorphism in the Sporothrix schenckii complex.
Sporotrichosis is a polymorphic disease caused by a complex of thermodimorphic fungi including S. brasiliensis, S. schenckii sensu stricto (s. str.), S. globosa and S. luriei. Humans and animals can acquire the disease through traumatic inoculation of propagules into the subcutaneous tissue. Despite the importance of sporotrichosis as a disease that can take epidemic proportions there are just a few studies dealing with genetic polymorphisms and genomic architecture of these pathogens. The main objective of this study was to investigate chromosomal polymorphisms and genomic organization among different isolates in the S. schenckii complex. We used pulsed field gel electrophoresis (PFGE) to separate chromosomal fragments of isolated DNA, followed by probe hybridization. Nine loci (β-tubulin, calmodulin, catalase, chitin synthase 1, Internal Transcribed Spacer, Pho85 cyclin-dependent kinase, protein kinase C Ss-2, G protein α subunit and topoisomerase II) were mapped onto chromosomal bands of Brazilian isolates of S. schenckii s. str. and S. brasiliensis. Our results revealed the presence of intra and interspecies polymorphisms in chromosome number and size. The gene hybridization analysis showed that closely related species in phylogenetic analysis had similar genetic organizations, mostly due to identification of synteny groups in chromosomal bands of similar sizes. Our results bring new insights into the genetic diversity and genome organization among pathogenic species in the Sporothrix schenckii complex.
Discipline of Cellular Biology, Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo, São Paulo, São Paulo, Brazil.
Alexandre A
AA
Sasaki
Discipline of Cellular Biology, Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo, São Paulo, São Paulo, Brazil.
Geisa F
GF
Fernandes
Discipline of Cellular Biology, Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo, São Paulo, São Paulo, Brazil.
Anderson M
AM
Rodrigues
Discipline of Parasitology, Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo, São Paulo, São Paulo, Brazil.
Fábio M
FM
Lima
Discipline of Parasitology, Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo, São Paulo, São Paulo, Brazil.
Marjorie M
MM
Marini
Biomedical Engineering Center, Camilo Castelo Branco University, São Paulo, São Paulo, Brazil.
Luciano
L
Dos S Feitosa
Department of Cell Biology, Biological Sciences Institute, University of Brasília, Brasília, Distrito Federal, Brazil.
Marcus
M
de Melo Teixeira
Department of Cell Biology, Biological Sciences Institute, University of Brasília, Brasília, Distrito Federal, Brazil ; Genomic Sciences and Biotechnology, Catholic University of Brasília, Brasília, Distrito Federal, Brazil.
Maria Sueli Soares
MS
Felipe
Discipline of Parasitology, Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo, São Paulo, São Paulo, Brazil.
José Franco
JF
da Silveira
Discipline of Cellular Biology, Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo, São Paulo, São Paulo, Brazil.
Zoilo P
ZP
de Camargo
D015139:Blotting, Southern; D015825:Chromosomes, Fungal / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D005819:Genetic Markers; D014644:Genetic Variation; D007621:Karyotyping; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D013175:Sporothrix / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*; D013174:Sporotrichosis / Q000235:genetics / Q000382:microbiology*; D014774:Virulence / Q000235:genetics*
D004271:DNA, Fungal; D005819:Genetic Markers