Inhibition of Epstein-Barr virus lytic cycle by an ethyl acetate subfraction separated from Polygonum cuspidatum root and its major component, emodin.
Polygonum cuspidatum is widely used as a medicinal herb in Asia. In this study, we examined the ethyl acetate subfraction F3 obtained from P. cuspidatum root and its major component, emodin, for their capacity to inhibit the Epstein-Barr virus (EBV) lytic cycle. The cell viability was determined by the MTT [3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyltetrazolium bromide] method. The expression of EBV lytic proteins was analyzed by immunoblot, indirect immunofluorescence and flow cytometric assays. Real-time quantitative PCR was used to assess the EBV DNA replication and the transcription of lytic genes, including BRLF1 and BZLF1. Results showed that the F3 and its major component emodin inhibit the transcription of EBV immediate early genes, the expression of EBV lytic proteins, including Rta, Zta, and EA-D and reduces EBV DNA replication, showing that F3 and emodin are potentially useful as an anti-EBV drug.
Department of Otolaryngology, Chi Mei Medical Center, Liouying, Tainan 717, Taiwan.
Ching-Yi
CY
Yiu
Department of Otolaryngology, Chi Mei Medical Center, Liouying, Tainan 717, Taiwan.
Shih-Ying
SY
Chen
Department of Otolaryngology, Chi Mei Medical Center, Liouying, Tainan 717, Taiwan.
Tsai-Hsiu
TH
Yang
Department of Otolaryngology, Chi Mei Medical Center, Liouying, Tainan 717, Taiwan.
Che-Jung
CJ
Chang
Department of Otolaryngology, Chi Mei Medical Center, Liouying, Tainan 717, Taiwan.
Dong-Bor
DB
Yeh
Department of Otolaryngology, Chi Mei Medical Center, Liouying, Tainan 717, Taiwan.
Yi-Jie
YJ
Chen
Department of Otolaryngology, Chi Mei Medical Center, Liouying, Tainan 717, Taiwan. tplin007@mail.chna.edu.tw.
Tsuey-Pin
TP
Lin
D000085:Acetates / Q000737:chemistry; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics; D004642:Emodin / Q000302:isolation & purification / Q000494:pharmacology*; D045468:Fallopia japonica / Q000737:chemistry*; D017781:Genes, Immediate-Early; D004854:Herpesvirus 4, Human / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D014158:Transcription, Genetic / Q000187:drug effects; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects
D000085:Acetates; D000998:Antiviral Agents; D004279:DNA, Viral; D010936:Plant Extracts; D012997:Solvents; D014764:Viral Proteins; C007650:ethyl acetate; D004642:Emodin