Hepatitis C virus genotypes: a plausible association with viral loads.
OBJECTIVE
The basic aim of this study was to find out the association of genotypes with host age, gender and viral load.


METHODS
The present study was conducted at Social Security Hospital, Pakistan. This study included 320 patients with chronic hepatitis C virus (HCV) infection who were referred to the hospital between November 2011 and July 2012. HCV viral detection and genotyping was performed and the association was seen between genotypes and host age, gender and viral load.


RESULTS
The analysis revealed the presence of genotypes 1 and 3 with further subtypes 1a, 1b, 3a, 3b and mixed genotypes 1b + 3a, 1b + 3b and 3a + 3b. Viral load quantification was carried out in all 151 HCV ribonucleic acid (RNA) positive patients. The genotype 3a was observed in 124 (82.12%) patients, 3b was found in 21 (13.91%), 1a was seen in 2 (1.32%), 1b in 1 (0.66%), mixed infection with 1b + 3a in 1 (0.66%), 1b + 3b in 1 (0.66%) and 3a + 3b was also found in 1 (0.66%) patient. Viral load quantification was carried out in all 151 HCV RNA positive patients and was compared between the various genotypes. The mean viral load in patients infected with genotype 1a was 2.75 × 10 6 , 1b 3.9 × 10 6 , 3a 2.65 × 10 6 , 3b 2.51 × 10 6 , 1b + 3a 3.4 × 106, 1b + 3b 2.7 × 106 and 3a + 3b 3.5 × 10 6 . An association between different types of genotypes and viral load was observed.


CONCLUSIONS
Further studies should be carried out to determine the association of viral load with different genotypes so that sufficient data is available and can be used to determine the type and duration of therapy needed and predict disease outcome.
Salma Ghulam
SG
Nabi
Ghazal
G
Zaffar
Nadeem Islam
NI
Sheikh
Khalid
K
Hassan
Department of Pathology, Shaukat Khanum Memorial Cancer Hospital and Research Centre, Lahore, Pakistan.
Usman
U
Hassan
D000328:Adult; D000368:Aged; D005260:Female; D005838:Genotype; D016174:Hepacivirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D019698:Hepatitis C, Chronic / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010154:Pakistan; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D019562:Viral Load; D055815:Young Adult
D012367:RNA, Viral