[Preliminary establishment of an electrochemiluminescence immunoassay method for detection of influenza virus B].
To establish an electrochemiluminescence immunoassay (ECLIA) for the detection of influenza virus B (Flu B), we biotinylated the Flu B monoclonal antibody (mAb),labeled the paired Ab with ruthenium and used the streptavidin-coated microparticles in this study. The ECL intensity was linear with the concentrations of antigen of Flu B in the range of 0.55 microg/mL to 17.5 microg/mL, with a detection limit of 0.55 microg/mL (S/N= 3). The precision,sensitivity and specificity were evaluated. The established ECLIA for Flu B antigen in this study was specific, sensitive and precise. It may provide a convenient and rapid technique for clinical detection of Flu B.
Department of Medical Research, General Hospital of Guangzhou Military Command, Guangzhou 510010, China.
Ran
R
Chen
Department of Medical Research, General Hospital of Guangzhou Military Command, Guangzhou 510010, China.
Jie
J
Wang
Department of Medical Research, General Hospital of Guangzhou Military Command, Guangzhou 510010, China.
Zhongming
Z
Liu
D000914:Antibodies, Viral / Q000032:analysis*; D055664:Electrochemical Techniques; D007118:Immunoassay / Q000379:methods*; D009981:Influenza B virus / Q000302:isolation & purification*; D049449:Luminescence
D000914:Antibodies, Viral