The hOGG1 Ser326Cys gene polymorphism and the risk of coronary ectasia in the Chinese population.
Oxidative stress (OS) is related to vascular inflammation possibly, contributing to the development of coronary ectasia (CE). Base excision repair (BER) and nucleotide excision repair are the main DNA repair pathways that can help to remove 8-hydroxydeoxyguanine (8-OHdG), a marker of OS. Human 8-oxoguanine DNA glycosylase 1 (hOGG1) is a key enzyme of the BER pathway and catalyzes the removal of 8-OHdG. The aim of our study was to investigate the association between hOGG1 Ser326Cys gene polymorphism and CE in a Chinese population. Five-hundred forty-seven patients who underwent diagnostic coronary angiography in a tertiary medical center were recruited. The angiographic definition of CE is the diameter of the ectatic segment being more than 1.5 times larger compared with an adjacent healthy reference segment. The gene polymorphisms were analyzed by polymerase chain reaction. The urine 8OHdG concentration was measured using a commercial ELISA kit. The distribution of hOGG1 Ser326Cys genotypes was significantly different between CE and non-CE groups (p = 0.033). The odds ratio of CE development for the Ser to the Cys variant was 1.55 (95% confidence interval (CI), 1.04-2.31, p = 0.033). Both univariate and logistic regression analysis showed a significant association of hOGG1 Ser326Cys polymorphism in the dominant model with CE development (p = 0.009 and 0.011, respectively). Urine 8-OHdG levels were significantly higher in subjects carrying the hOGG1 Ser variant than in those with the Cys/Cys genotype (p < 0.03). In conclusion, our study suggests that the hOGG1 Ser326Cys gene variant might play a role in susceptibility to the development of CE.
Division of Cardiology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan. pochao.hsu@gmail.com.
Po-Chao
PC
Hsu
Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. bizibear@pchome.com.tw.
Chiao-Ling
CL
Wang
Division of Cardiology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan. cobeshm@seed.net.tw.
Ho-Ming
HM
Su
Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan. hjuo@kmu.edu.tw.
Suh-Hang
SH
Juo
Division of Cardiology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan. lth@kmu.edu.tw.
Tsung-Hsien
TH
Lin
Division of Cardiology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan. d750078@kmu.edu.tw.
Wen-Chol
WC
Voon
Department of Internal Medicine, Faculty of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. sjshin@cc.kmu.edu.tw.
Shyi-Jang
SJ
Shin
Division of Cardiology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan. wtlai@cc.kmu.edu.tw.
Wen-Ter
WT
Lai
Division of Cardiology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan. sheush@cc.kmu.edu.tw.
Sheng-Hsiung
SH
Sheu
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000368:Aged; D016022:Case-Control Studies; D002681:China; D003324:Coronary Artery Disease / Q000000981:diagnostic imaging / Q000235:genetics*; D045647:DNA Glycosylases / Q000235:genetics*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D004108:Dilatation, Pathologic / Q000000981:diagnostic imaging / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D020641:Polymorphism, Single Nucleotide; D011859:Radiography
D000080242:8-Hydroxy-2'-Deoxyguanosine; D045647:DNA Glycosylases; C479058:oxoguanine glycosylase 1, human; D003849:Deoxyguanosine