Neural progenitor cells from human induced pluripotent stem cells generated less autogenous immune response.
The breakthrough development of induced pluripotent stem cells (iPSCs) raises the prospect of patient-specific treatment for many diseases through the replacement of affected cells. However, whether iPSC-derived functional cell lineages generate a deleterious immune response upon auto-transplantation remains unclear. In this study, we differentiated five human iPSC lines from skin fibroblasts and urine cells into neural progenitor cells (NPCs) and analyzed their immunogenicity. Through co-culture with autogenous peripheral blood mononuclear cells (PBMCs), we showed that both somatic cells and iPSC-derived NPCs do not stimulate significant autogenous PBMC proliferation. However, a significant immune reaction was detected when these cells were co-cultured with allogenous PBMCs. Furthermore, no significant expression of perforin or granzyme B was detected following stimulation of autogenous immune effector cells (CD3(+)CD8(-) T cells, CD3(+)CD8(+) T cells or CD3(-)CD56(+) NK cells) by NPCs in both PBMC and T cell co-culture systems. These results suggest that human iPSC-derived NPCs may not initiate an immune response in autogenous transplants, and thus set a base for further preclinical evaluation of human iPSCs.
Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China.
Ke
K
Huang
PengFei
P
Liu
Xiang
X
Li
ShuBin
S
Chen
LiHui
L
Wang
Li
L
Qin
ZhengHui
Z
Su
WenHao
W
Huang
Juli
J
Liu
Bei
B
Jia
Jie
J
Liu
JingLei
J
Cai
DuanQing
D
Pei
GuangJin
G
Pan
D015703:Antigens, CD / Q000276:immunology; D001483:Base Sequence; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D053804:Granzymes / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000276:immunology; D058953:Neural Stem Cells / Q000166:cytology* / Q000276:immunology; D054353:Perforin / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D033581:Stem Cell Transplantation
D015703:Antigens, CD; D017931:DNA Primers; D054353:Perforin; D053804:Granzymes