Determination of 2-methoxyestradiol in serum samples and pharmaceutical preparations by silver nanoparticles-enhanced chemiluminescence.
Silver nanoparticles (AgNPs) exhibited better chemiluminescence (CL) catalysis activity and smaller nanoparticles have stronger catalysis ability in luminol-K3Fe(CN)6 system among the synthesized AgNPs of different size. 10±2 nm nanoparticles was used as catalysts to enhance the reaction sensitivity. It was found that the CL intensity of AgNPs-luminol-K3Fe(CN)6 was strongly inhibited in the presence of 2-methoxyestradiol (2-ME) and the relative CL intensity was in linear correlation with the concentration of 2-ME. Thus, the silver nanoparticles-enhanced CL method for the determination of 2-ME was developed. The proposed method has a detection limit (3 Sb/K) of 5.0×10(-10) mol L(-1) with a relative standard deviation of 0.75% for 5.0×10(-8) mol L(-1) 2-ME. The method was successfully applied for determination of 2-ME in human serum and pharmaceutical preparations. The possible CL reaction mechanism was also discussed briefly. Oxygen radicals played an important role in the catalytic process.
The Third People's Hospital of Qingdao, Qingdao, China; School of Pharmaceutical Science, Zhengzhou University, Zhengzhou, China.
Min
M
Zhang
School of Pharmaceutical Science, Zhengzhou University, Zhengzhou, China.
Xiangqin
X
Xiao
School of Pharmaceutical Science, Zhengzhou University, Zhengzhou, China.
Wenyuan
W
Zeng
School of Pharmaceutical Science, Zhengzhou University, Zhengzhou, China.
Xiaoying
X
Zeng
School of Pharmaceutical Science, Zhengzhou University, Zhengzhou, China. Electronic address: yhch@zzu.edu.cn.
Hanchun
H
Yao
D000077584:2-Methoxyestradiol; D002384:Catalysis; D004958:Estradiol / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood; D006801:Humans; D057230:Limit of Detection; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D050257:Tubulin Modulators / Q000032:analysis / Q000097:blood*
D004364:Pharmaceutical Preparations; D050257:Tubulin Modulators; D012834:Silver; D004958:Estradiol; D008165:Luminol; D000077584:2-Methoxyestradiol
2-methoxyestradiol; Chemiluminescence; Serum samples; Silver nanoparticles