Focal adhesion protein expression in human diffuse large B-cell lymphoma.
OBJECTIVE
Focal adhesions have been associated with poor prognosis in multiple cancer types, but their prognostic value in diffuse large B-cell lymphoma (DLBCL) has not been evaluated. The aim of this study was to investigate the expression patterns and the prognostic value of the focal adhesion proteins FAK, Pyk2, p130Cas and HEF1 in DLBCL.


RESULTS
Focal adhesion protein expression was examined using immunohistochemistry in normal lymphoid tissues and in 60 DLBCL patient samples. Kaplan-Meier survival and Cox regression analysis were performed to evaluate the correlation of focal adhesion protein expression with patient prognosis. FAK, Pyk2, p130Cas and HEF1 expression was mostly found in the germinal centres of normal human lymphoid tissues. When assessed in DLBCL samples, FAK, Pyk2, p130Cas and HEF1 were highly expressed in 45%, 34%, 42% and 45% of the samples, respectively. Multivariate Cox analysis revealed that decreased FAK expression was a significant independent predictor of poorer disease outcome.


CONCLUSIONS
FAK expression is an independent prognostic factor in DLBCL. Our results suggest that the addition of FAK immunostaining to the current immunohistochemical algorithms may facilitate risk stratification of DLBCL patients.
Grup d'Oncogènesi i Antitumorals, Institut d'Investigacions Biomèdiques Sant Pau, Barcelona, Spain.
Rosa
R
Bosch
Rebeca
R
Dieguez-Gonzalez
María José
MJ
Moreno
Alberto
A
Gallardo
Silvana
S
Novelli
Iñigo
I
Espinosa
María Virtudes
MV
Céspedes
Miguel Ángel
MÁ
Pavón
Javier
J
Briones
Albert
A
Grañena
Jorge
J
Sierra
Ramon
R
Mangues
Isolda
I
Casanova
D048868:Adaptor Proteins, Signal Transducing / Q000096:biosynthesis*; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D050719:Crk-Associated Substrate Protein / Q000096:biosynthesis*; D018572:Disease-Free Survival; D005260:Female; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D016403:Lymphoma, Large B-Cell, Diffuse / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D010750:Phosphoproteins / Q000096:biosynthesis*; D016016:Proportional Hazards Models; D046888:Tissue Array Analysis
D048868:Adaptor Proteins, Signal Transducing; C493841:BCAR1 protein, human; D014408:Biomarkers, Tumor; D050719:Crk-Associated Substrate Protein; C099942:NEDD9 protein, human; D010750:Phosphoproteins; D051416:Focal Adhesion Protein-Tyrosine Kinases
diffuse; focal adhesions; large B-cell lymphoma; prognosis