Neolactoferrin as a stimulator of innate and adaptive immunity.
The effect of the innovative product Neolactoferrin, a natural combination of recombinant human lactoferrin (90%) and goat lactoferrin (10%) isolated from the milk of transgenic goats carrying the full-length human lactoferrin gene, on human immune system cells was studied. Neolactoferrin enhanced the production of IL-1β. Neolactoferrin saturated with iron ions increased the synthesis of pro-inflammatory cytokine TNFα. It determined the direction of the differentiation of precursor dendrite cells. Under the action of T cells, Neolactoferrin amplified the expression of the transcription factors responsible for the differentiation of Th- and Treg-cells and stimulated the production of both IFNγ and IL-4. The results suggest that Neolactoferrin exhibits an immunotropic activity and hinders the development of immune inflammatory processes. Iron saturation of Neolactoferrin increases its pro-inflammatory activity.
Institute of Immunology, Federal Medical and Biological Agency, Kashirskoe shosse, 24/2, Moscow, Russia, 115478 ; Institute of Gene Biology, Russian Academy of Sciences, Vavilova Str., 34/5, Moscow, Russia, 119334.
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Chernousov
Institute of Immunology, Federal Medical and Biological Agency, Kashirskoe shosse, 24/2, Moscow, Russia, 115478.
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Nikonova
Institute of Immunology, Federal Medical and Biological Agency, Kashirskoe shosse, 24/2, Moscow, Russia, 115478.
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Sharova
Institute of Immunology, Federal Medical and Biological Agency, Kashirskoe shosse, 24/2, Moscow, Russia, 115478.
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Mitin
Institute of Immunology, Federal Medical and Biological Agency, Kashirskoe shosse, 24/2, Moscow, Russia, 115478.
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Litvina
Institute of Immunology, Federal Medical and Biological Agency, Kashirskoe shosse, 24/2, Moscow, Russia, 115478.
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Sadchikov
Institute of Gene Biology, Russian Academy of Sciences, Vavilova Str., 34/5, Moscow, Russia, 119334.
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Goldman
Institute of Gene Biology, Russian Academy of Sciences, Vavilova Str., 34/5, Moscow, Russia, 119334.
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Yarilin
Institute of Immunology, Federal Medical and Biological Agency, Kashirskoe shosse, 24/2, Moscow, Russia, 115478.
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Sadchikova
Neolactoferrin; Recombinant human lactoferrin; cytokines; immunity; inflammation; transcription factors