Association study of human leukocyte antigen-DRB1 alleles with rheumatoid arthritis in Algerian patients.
BACKGROUND
Rheumatoid arthritis (RA) is a chronic, systemic, inflammatory and multifactorial disease. Genetic predisposition seems to play an important role. The aim of this study is to explore the relationship between human leukocyte antigen (HLA)-DRB1 alleles and susceptibility, clinical and biological features of RA in an Algerian patient population.


METHODS
Using polymerase chain reaction - sequence specific primers (SSP), 134 RA patients and 132 healthy controls were genotyped for HLA-DRB1 and HLA-DRB1*04 subtypes.


RESULTS
HLA-DRB1*04 was found to have increased frequency in the RA group compared to controls (P < 0.001, OR = 3.14), and was associated with anti-citrullinated protein antibodies positivity (ACPA) (P = 0.01, OR = 2.35). In contrast, HLA-DRB1*07 was found to have a decreased frequency in patients compared to controls (P = 0.003, OR = 0.44) and significant decrease was observed in patients with the rheumatoid factor (RF) positivity subgroup (P = 0.009, OR = 0.29). HLA-DRB1*04:05 was associated with RA (P = 0.005, OR = 3.41), whereas, HLA-DRB1*04:02 showed a protective effect against RA (P = 0.003, OR = 0.20).


CONCLUSIONS
HLA-DRB1*04 was associated with increased risk for RA and ACPA positivity, while HLA-DRB1*07 was associated with reduced risk for RA and RF synthesis in Algerian patients.
Department of Immunology, CHU Béni-Messous, Algiers, Algeria.
Réda
R
Djidjik
Department of Immunology, CHU Béni-Messous, Algiers, Algeria.
Ines
I
Allam
Department of Immunology, CHU Béni-Messous, Algiers, Algeria.
Sanaa
S
Douaoui
Department of Immunology, Central Hospital of Army, Algiers, Algeria.
Yanis
Y
Meddour
Department of Immunology, CHU Béni-Messous, Algiers, Algeria.
Khaled
K
Cherguelaîne
Department of Immunology, CHU Béni-Messous, Algiers, Algeria.
Azzedine
A
Tahiat
Department of Immunology, CHU Béni-Messous, Algiers, Algeria.
Nabil
N
Raaf
Department of Rheumatology, EPH Benaknoun, Algiers, Algeria.
Amina
A
Abdessemed
Department of Rheumatology, EPH Benaknoun, Algiers, Algeria.
Naouel
N
Khaldoun
Department of Rheumatology, CHU Béni-Messous, Algiers, Algeria.
Naima
N
Bahaz
Department of Immunology, Central Hospital of Army, Algiers, Algeria.
Samia
S
Chaib
Department of Rheumatology, EPH Benaknoun, Algiers, Algeria.
Aicha
A
Ladjouze-Rezig
Department of Immunology, CHU Béni-Messous, Algiers, Algeria.
Mohamed
M
Ghaffor
D000328:Adult; D000462:Algeria; D000075422:Anti-Citrullinated Protein Antibodies / Q000097:blood; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D059811:HLA-DRB1 Chains / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D010641:Phenotype; D065840:Protective Factors; D012217:Rheumatoid Factor / Q000097:blood; D012307:Risk Factors; D055815:Young Adult
D000075422:Anti-Citrullinated Protein Antibodies; D015415:Biomarkers; D005819:Genetic Markers; D059811:HLA-DRB1 Chains; D012217:Rheumatoid Factor
HLA-DRB1 alleles; anti-cyclic peptides antibodies; rheumatoid arthritis; rheumatoid factor