Osteogenetic property of a biodegradable three-dimensional macroporous hydrogel coating on titanium implants fabricated via EPD.
The potential for a successful integration of implants with surrounding tissue may be jeopardized in a number of compromised conditions. Biochemical surface modification is one of the choices to extend the spectrum of indications. We have previously successfully fabricated chitosan-gelatin (CS/G) coatings on a titanium surface via electrophoretic deposition, which may be promising candidates for further loading of functional agents. In this study, we have identified the microstructure, physicochemical properties and biological performance of CS/G coatings in vitro and in vivo. The in vitro degradation test indicated that CS/G coatings in the presence of lysozyme showed a significant weight loss after 28 days. The results of the cell culture exhibited that CS/G coatings could sustain MC3T3-E1 cell attachment, proliferation and migration. In vivo osteogenetic behavior evaluated by Micro-CT and histomorphometrical analysis revealed significant new bone formation around CS/G implants at 8 and 12 weeks, compared to sandblasted/acid-etched implants. Moreover, histological evaluation suggested the majority of CS/G coatings were degraded at 12 weeks. Therefore, we have concluded that the three-dimensional porous structure of scaffold-like CS/G coatings may facilitate osteogenesis and that such coatings can be biodegraded in the early bone healing process.
Key Laboratory for Oral Biomedical Engineering, Ministry of Education, School and Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan 430079, People's Republic of China.
Kena
K
Ma
Xinjie
X
Cai
Yi
Y
Zhou
Zhen
Z
Zhang
Tao
T
Jiang
Yining
Y
Wang
D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000737:chemistry; D000818:Animals; D001842:Bone and Bones; D048271:Chitosan / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D008297:Male; D051379:Mice; D009113:Muramidase / Q000737:chemistry; D016348:Osseointegration; D010012:Osteogenesis / Q000502:physiology*; D016062:Porosity; D019736:Prostheses and Implants; D011817:Rabbits; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D055114:X-Ray Microtomography
D020099:Coated Materials, Biocompatible; D020100:Hydrogels; D005780:Gelatin; D048271:Chitosan; D014025:Titanium; D000469:Alkaline Phosphatase; D009113:Muramidase