ProbMetab: an R package for Bayesian probabilistic annotation of LC-MS-based metabolomics.
We present ProbMetab, an R package that promotes substantial improvement in automatic probabilistic liquid chromatography-mass spectrometry-based metabolome annotation. The inference engine core is based on a Bayesian model implemented to (i) allow diverse source of experimental data and metadata to be systematically incorporated into the model with alternative ways to calculate the likelihood function and (ii) allow sensitive selection of biologically meaningful biochemical reaction databases as Dirichlet-categorical prior distribution. Additionally, to ensure result interpretation by system biologists, we display the annotation in a network where observed mass peaks are connected if their candidate metabolites are substrate/product of known biochemical reactions. This graph can be overlaid with other graph-based analysis, such as partial correlation networks, in a visualization scheme exported to Cytoscape, with web and stand-alone versions.
LabPIB, Department of Computing and Mathematics FFCLRP-USP, University of Sao Paulo, Ribeirao Preto, Brazil, INRA UMR1331, Toxalim, Research Centre in Food Toxicology, Universit de Toulouse, INSA, UPS, INP; LISBP, Toulouse, France, Institute for Systems Biology, Seattle, Washington, USA, CNRS, UMR5504, Toulouse, France, Department of Genetics ESALQ-USP, University of Sao Paulo, Piracicaba, Brazil and Laboratorio Nacional de Ciencia e Tecnologia do Bioetanol CTBE, Campinas, Brazil.
Ricardo R
RR
Silva
Fabien
F
Jourdan
Diego M
DM
Salvanha
Fabien
F
Letisse
Emilien L
EL
Jamin
Simone
S
Guidetti-Gonzalez
Carlos A
CA
Labate
Ricardo Z N
RZ
Vêncio
D057205:Automation, Laboratory; D001499:Bayes Theorem; D002853:Chromatography, Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D012984:Software