Protective effect of diosmin against diabetic neuropathy in experimental rats.
OBJECTIVE
The present study was undertaken to evaluate the effect of diosmin in diabetic neuropathy in type 2 diabetic rats.


METHODS
Type 2 diabetes was induced in male Sprague-Dawley rats by single intraperitoneal injection of streptozotocin (35 mg/kg) and high-fat diet. Four weeks after the confirmation of diabetes, diabetic rats were treated with diosmin (50 and 100 mg/kg, p.o.) for next 4 weeks. Rats were evaluated for biochemical, behavioral and oxidative stress parameters. Eddy's hot plate and tail immersion test were performed on 6th, 7th, 8th, 9th and 10th weeks of experiment to assess thermal hyperalgesia and cold allodynia respectively. Further, the walking function test was performed for assessing the motor responses at the end of the treatment schedule.


RESULTS
Rats were fed with high-fat diet throughout the experiment schedule and administration of low-dose streptozotocin induced significant elevation in blood glucose level and insulin resistance which was confirmed by oral glucose tolerance test. Treatment with diosmin at doses of 50 and 100 mg/kg significantly restored the reduced body weight, elevated blood sugar and lipid profiles. Further the dose-dependent improvement was observed in thermal hyperalgesia, cold allodynia and walking function in diabetic rats treated with diosmin. Elevated levels of malondialdehyde, and nitric oxide and decreased glutathione levels and superoxide dismutase activity in diabetic rats were restored significantly after the 4 weeks of diosmin treatment.


CONCLUSIONS
Diosmin has shown beneficial effect in preventing the progression of early diabetic neuropathy in rats.
Department of Pharmacology, Sinhgad College of Pharmacy, Vadgaon (Bk.), Pune 411041, India; E-mail: dilpeshjain@yahoo.com.
Dilpesh
D
Jain
Department of Pharmacology, Sinhgad College of Pharmacy, Vadgaon (Bk.), Pune 411041, India.
Manish Kumar
MK
Bansal
Department of Pharmacology, Sinhgad College of Pharmacy, Vadgaon (Bk.), Pune 411041, India.
Rahul
R
Dalvi
Department of Pharmacology, SSDJ College of Pharmacy, Chandwad, Nashik 423101, India.
Aman
A
Upganlawar
Department of Research, Suresh Gyan Vihar University, Mahal Jagtpura, Jaipur 302025, India.
Rahul
R
Somani
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D002957:Citrus / Q000737:chemistry; D003929:Diabetic Neuropathies / Q000188:drug therapy / Q000378:metabolism / Q000517:prevention & control*; D004145:Diosmin / Q000008:administration & dosage*; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley
D001786:Blood Glucose; D010936:Plant Extracts; D008315:Malondialdehyde; D004145:Diosmin; D002784:Cholesterol; D005978:Glutathione