Dichloromethane removal and microbial variations in a combination of UV pretreatment and biotrickling filtration.
Biofiltration of hydrophobic and/or recalcitrant volatile organic compounds in industry is currently limited. A laboratory-scale system integrating ultraviolet (UV) photodegradation and a biotrickling filter (BTF) was developed to treat dichloromethane (DCM), and this was compared to BTF alone. A combined UV-BTF approach permitted faster biofilm formation and greater removal than BTF. DCM distribution and its photodegradation intermediates revealed that the lower filter of the UV-BTF contributed more to CO2 production; the upper filter assisted more with DCM removal. The UV-BTF kept secretion of extracellular polymeric substances at a normal level with an evenly distributed biomass. Pyrosequencing analysis showed that the dominant population in the combined biofilter was more diverse than that in BTF alone. Our data provide a foundation for understanding the effect of UV pretreatment on BTF performance and the microbial community.
Collaborative Innovation Center of Green Pharmaceutical Engineering, Zhejiang University of Technology, Hangzhou, China.
Yu
Y
Jianming
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, China.
Liu
L
Wei
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, China. Electronic address: zwcheng@zjut.edu.cn.
Cheng
C
Zhuowei
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, China.
Jiang
J
Yifeng
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, China.
Cai
C
Wenji
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, China. Electronic address: jchen@zjut.edu.cn.
Chen
C
Jianmeng
D001673:Biodegradation, Environmental; D044822:Biodiversity; D018441:Biofilms / Q000145:classification* / Q000254:growth & development / Q000528:radiation effects; D019149:Bioreactors / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005374:Filtration; D008752:Methylene Chloride / Q000737:chemistry / Q000302:isolation & purification* / Q000528:radiation effects; D010084:Oxidation-Reduction; D010802:Phylogeny; D014466:Ultraviolet Rays; D055549:Volatile Organic Compounds / Q000737:chemistry / Q000302:isolation & purification* / Q000528:radiation effects
D004269:DNA, Bacterial; D055549:Volatile Organic Compounds; D008752:Methylene Chloride
Biotrickling filtration; Extracellular polymeric substances; Microbial community; Pyrosequencing; Target distribution; UV photodegradation