Curcumin as a modulator of oxidative stress during storage: a study on plasma.
BACKGROUND
Plasma reflects the situations of oxidative stress in blood. Curcumin as an additive in storage solutions is not explored.


METHODS
Blood was drawn from male Wistar rats (4 months old) and stored over a period of 20 days at 4 °C in CPDA-1. Plasma was isolated from stored blood at regular intervals. Oxidative stress markers were analyzed in controls and experimental samples (with curcumin 10mM, 30 mM and 60mM).


RESULTS
Antioxidant enzymes and sulfhydryls ameliorated, while TBARS and protein carbonyls reduced in curcumin samples. However, curcumin could not protect the susceptible groups in proteins thereby leading to dityrosine linkages (AOPP).


CONCLUSIONS
Curcumin was beneficial at higher concentrations as it reduced oxidative stress, therefore paving way for the further studies on curcumin as a component in storage solutions.
Department of Biotechnology, Center for Post Graduate Studies, Jain University, #18/3, 9th Main, 3rd Block, Jayanagar, Bangalore 560011, India.
Hsieh
H
Carl
Department of Biotechnology, Center for Post Graduate Studies, Jain University, #18/3, 9th Main, 3rd Block, Jayanagar, Bangalore 560011, India.
Ahuja
A
Chandni
Department of Biotechnology, Center for Post Graduate Studies, Jain University, #18/3, 9th Main, 3rd Block, Jayanagar, Bangalore 560011, India.
Kumari
K
Neha
Department of Biotechnology, Center for Post Graduate Studies, Jain University, #18/3, 9th Main, 3rd Block, Jayanagar, Bangalore 560011, India.
Sinha
S
Trishna
Department of Biotechnology, Center for Post Graduate Studies, Jain University, #18/3, 9th Main, 3rd Block, Jayanagar, Bangalore 560011, India. Electronic address: tiwari.vani@gmail.com.
Rajashekharaiah
R
Vani
D000818:Animals; D000975:Antioxidants / Q000737:chemistry; D001793:Blood Preservation; D003474:Curcumin / Q000737:chemistry / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010949:Plasma / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
D000975:Antioxidants; D004791:Enzyme Inhibitors; D003474:Curcumin
Curcumin; Oxidative stress; Plasma; Storage