Morphology and phylogeny of Henneguya jocu n. sp. (Myxosporea, Myxobolidae), infecting the gills of the marine fish Lutjanus jocu.
Henneguya jocu n. sp. (Myxosporea, Myxobolidae) is described from the gill lamellae of the marine teleost fish Lutjanus jocu, with a focus on ultrastructural and molecular features. This myxosporean forms subspherical cysts up to ∼260 μm × 130 μm long, and develops asynchronously. Mature myxospores ellipsoidal with a bifurcated caudal process. Myxospore length 10.9 ± 0.4 μm (n=50); width, 8.2 ± 0.3 μm (n=50); and thickness, 2.9 ± 0.5 μm (n=50). Two equal caudal processes, 34.1±1.0 μm long (n=50); and total myxospore length, 45.2 ± 1.0 μm (n=50). Two symmetric valves surround two ellipsoidal polar capsules, 5.0 ± 0.3 × 1.4 ± 0.2 μm (n=20), each containing an isofilar polar filament forming 4-5 coils along the inner wall of these structures, as well as a binucleated sporoplasm presenting a spherical vacuole and several globular sporoplasmosomes. Both the morphological data and molecular analysis of the SSU rDNA gene identify this parasite as a new species of the genus Henneguya. Maximum Likelihood and Maximum Parsimony analyses further indicate that the parasite clusters within others marine Myxobolidae species, forming a group alongside other Henneguya species described from marine hosts.
Laboratory of Cell Biology, Institute of Biomedical Sciences (ICBAS/UP), University of Porto, 4050-313 Porto, Portugal; Laboratory of Animal Pathology, Interdisciplinary Centre of Marine and Environmental Research (CIIMAR/UP), University of Porto, 4050-123 Porto, Portugal; Zoology Department, College of Science, King Saud University, 11451 Riyadh, Saudi Arabia. Electronic address: azevedoc@icbas.up.pt.
Carlos
C
Azevedo
Laboratory of Cell Biology, Institute of Biomedical Sciences (ICBAS/UP), University of Porto, 4050-313 Porto, Portugal; Laboratory of Animal Pathology, Interdisciplinary Centre of Marine and Environmental Research (CIIMAR/UP), University of Porto, 4050-123 Porto, Portugal.
Sónia
S
Rocha
Edilson Matos Research Laboratory, Federal University of Pará (UFPa), 66000 Belém, PA, Brazil.
Patrícia
P
Matos
Carlos Azevedo Research Laboratory, Federal Rural University of Amazonia (UFRA), 66077-530 Belém, PA, Brazil.
Edilson
E
Matos
Laboratory of Cell Biology, Institute of Biomedical Sciences (ICBAS/UP), University of Porto, 4050-313 Porto, Portugal.
Elsa
E
Oliveira
Zoology Department, College of Science, King Saud University, 11451 Riyadh, Saudi Arabia.
Saleh
S
Al-Quraishy
Laboratory of Animal Pathology, Interdisciplinary Centre of Marine and Environmental Research (CIIMAR/UP), University of Porto, 4050-123 Porto, Portugal; Department of Sciences, High Institute of Health Sciences-North (CESPU), 4585-116 Gandra, Portugal.
Graça
G
Casal
D000818:Animals; D004275:DNA, Ribosomal / Q000235:genetics; D005880:Gills / Q000469:parasitology*; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D055764:Myxozoa / Q000145:classification* / Q000166:cytology* / Q000235:genetics / Q000648:ultrastructure; D010473:Perciformes / Q000469:parasitology*; D010802:Phylogeny; D013045:Species Specificity
D004275:DNA, Ribosomal
Brazil; Henneguya; Myxozoa; New species; Phylogeny; Ultrastructure