In vitro evaluation of Bacopa monniera extract and individual constituents on human recombinant monoamine oxidase enzymes.
Bacopa monniera is a traditional Ayurvedic medicinal plant that has been used worldwide for its nootropic action. Chemically standardized extract of B. monniera is now available as over the counter herbal remedy to enhance memory in children and adults. Considering the nootropic action of B. monniera, we evaluated the effect of clinically available B. monniera extract and six of B. monniera constituents (bacoside A3, bacopaside I, bacopaside II, bacosaponin C, bacosine, and bacoside A mixture) on recombinant human monoamine oxidase (MAO) enzymes. The effect of B. monniera extract and individual constituents on human recombinant MAO-A and MAO-B enzymes was evaluated using MAO-Glo(TM) assay kit (Promega Corporation, USA), following the instruction manual. IC50 and mode of inhibition were measured for MAO enzymes. Bacopaside I and bacoside A mixture inhibited the MAO-A and MAO-B enzymes. Bacopaside I exhibited mixed mode of inhibition with IC50 and Ki values of 17.08 ± 1.64 and 42.5 ± 3.53 µg/mL, respectively, for MAO-A enzyme. Bacopaside I is the major constituent of B. monniera, which inhibited the MAO-A enzyme selectively.
Pharmacokinetics and Metabolism Division, CSIR-Central Drug Research Institute, Lucknow, 226001, India.
Rajbir
R
Singh
Rachumallu
R
Ramakrishna
Manisha
M
Bhateria
Rabi Sankar
RS
Bhatta
D034681:Bacopa / Q000737:chemistry*; D006801:Humans; D008995:Monoamine Oxidase / Q000378:metabolism*; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000378:metabolism; D012503:Saponins / Q000494:pharmacology*; D014315:Triterpenes / Q000494:pharmacology*
D008996:Monoamine Oxidase Inhibitors; D010936:Plant Extracts; D011994:Recombinant Proteins; D012503:Saponins; D014315:Triterpenes; C442170:bacopaside I; D008995:Monoamine Oxidase; C579458:monoamine oxidase A, human
Bacopa monniera; bacoside; monoamine oxidase