Altered expression of neuropeptide Y, Y1 and Y2 receptors, but not Y5 receptor, within hippocampus and temporal lobe cortex of tremor rats.
As an endogenous inhibitor of glutamate-mediated synaptic transmission in mammalian central nervous system, neuropeptide Y (NPY) plays a crucial role in regulating homeostasis of neuron excitability. Loss of balance between excitatory and inhibitory neurotransmission is thought to be a chief mechanism of epileptogenesis. The abnormal expression of NPY and its receptors observed following seizures have been demonstrated to be related to the production of epilepsy. The tremor rat (TRM) is a hereditary epileptic animal model. So far, there is no report concerning whether NPY and its receptors may be involved in TRM pathogenesis. In this study, we focused on the expression of NPY and its three receptor subtypes: Y1R, Y2R and Y5R in the TRM brain. We first found the expression of NPY in TRM hippocampus and temporal lobe cortex was increased compared with control (Wistar) rats. The mRNA and protein expression of Y1R was down-regulated in hippocampus but up-regulated in temporal lobe cortex, whereas Y2R expression was significantly increased in both areas. There was no significant change of Y5R expression in either area. The immunohistochemistry data showed that Y1R, Y2R, Y5R were present throughout CA1, CA3, dentate gyrus (DG) and the entorhinal cortex which is included in the temporal lobe cortex of TRM. In conclusion, our results showed the altered expression of NPY, Y1R and Y2R but not Y5R in hippocampus and temporal lobe cortex of TRM brain. This abnormal expression may be associated with the generation of epileptiform activity and provide a candidate target for treatment of genetic epilepsy.
Biochip Center, College of Basic Medicine, China Medical University, Shenyang 110001, China; Department of Neurology, The First Hospital of China Medical University, Shenyang 110001, China.
Xiaoxue
X
Xu
Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, Shenyang 110001, China.
Feng
F
Guo
Biochip Center, College of Basic Medicine, China Medical University, Shenyang 110001, China.
Qun
Q
He
Central Lab, The First Hospital of China Medical University, Shenyang 110001, China.
Xinze
X
Cai
Experiment Center of Traditional Chinese Medicine, The Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032, China.
Dongyu
D
Min
Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, Shenyang 110001, China.
Qianhui
Q
Wang
Biochip Center, College of Basic Medicine, China Medical University, Shenyang 110001, China.
Shaocheng
S
Wang
Biochip Center, College of Basic Medicine, China Medical University, Shenyang 110001, China.
Liu
L
Tian
Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, Shenyang 110001, China.
Jiqun
J
Cai
Biochip Center, College of Basic Medicine, China Medical University, Shenyang 110001, China. Electronic address: yjzhao@mail.cmu.edu.cn.
Yujie
Y
Zhao
D000818:Animals; D004195:Disease Models, Animal; D004827:Epilepsy / Q000235:genetics* / Q000503:physiopathology; D005260:Female; D006624:Hippocampus / Q000502:physiology; D008297:Male; D009478:Neuropeptide Y / Q000378:metabolism*; D011922:Rats, Mutant Strains; D017208:Rats, Wistar; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000378:metabolism; D018013:Receptors, Neuropeptide / Q000235:genetics* / Q000378:metabolism; D017476:Receptors, Neuropeptide Y / Q000235:genetics* / Q000378:metabolism; D013702:Temporal Lobe / Q000502:physiology; D014202:Tremor / Q000235:genetics / Q000503:physiopathology
D009478:Neuropeptide Y; C066211:Npy1r protein, rat; D043562:Receptors, G-Protein-Coupled; D018013:Receptors, Neuropeptide; D017476:Receptors, Neuropeptide Y; C095889:neuropeptide Y2 receptor; C100568:neuropeptide Y5 receptor
Genetic epilepsy; Neuropeptide Y; Receptor; Tremor rats