[Development of standardization platform for optical density value based on an improved method].
Due to the high variation in test results of indirect enzyme-linked immuno sorbent assay (ELISA) and complicated steps involved in the process of standardization, a platform used for standardizing the test results from indirect ELISA was developed. The platform was designed based on 'Improved Standardization Method for Optical Density' (I-STOD). Gauss-Newton iteration was applied to estimate parameters in a standard formula. Programming Language VB was used for developing interface of platform. The results indicated that the validity of experiment could be verified through platform. A well determined scope of standardization could be generated. The sample with concentration within the scope was standardized and the degree of dilution was calculated for those outside the scope. The platform was successfully developed which normalized the process of standardization. The function provides the researchers with an effective and convenient tool for quickly achieving standardization of ELISA test results.
School of Basic Medical Sciences, Nanjing Medical University, Nanjing 210029, China.
Xiaoming
X
Tu
Nanjing Red Cross Blood Center, Nanjing 210003, China.
Yilun
Y
Zhao
Nanjing Red Cross Blood Center, Nanjing 210003, China.
Yudong
Y
Dai
Nanjing Red Cross Blood Center, Nanjing 210003, China.
Xubing
X
Cai
School of Basic Medical Sciences, Nanjing Medical University, Nanjing 210029, China.
Jianping
J
Luo
School of International Education, Nanjing Medical University, Nanjing 210029, China.
Yu
Y
Zheng
D000906:Antibodies / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards*; D006801:Humans; D055620:Optical Phenomena
D000906:Antibodies