Evaluation of intensity and pulse width of different moderators for designing a new diffractometer for protein crystals with large unit cells in J-PARC/MLF.
We plan to design a high-resolution biomacromolecule neutron time-of-flight diffractometer, which allows us to collect data from crystals with unit cells above 250 Å, in the materials and life science experimental facility at the Japan Proton Accelerator Research Complex. This new diffractometer can be used for a detailed analysis of large proteins such as membrane proteins and supermolecular complex. A quantitative comparison of the intensity and pulse width of a decoupled moderator (DM) against a coupled moderator (CM) considering the pulse width time resolution indicated that the DM satisfies the criteria for our diffractometer rather than the CM. The results suggested that a characteristic feature of the DM, i.e., narrow pulse width with a short tail, is crucial for the separation of Bragg reflections from crystals with large unit cells. On the other hand, it should be noted that the weak signals from the DM are buried under the high-level background caused by the incoherent scattering of hydrogen atoms, especially, in the case of large unit cells. We propose a profile-fitting integration method combined with the energy loss functions and a background subtraction method achieved by employing the statistics-sensitive nonlinear iterative peak-clipping algorithm.
Quantum Beam Science Directorate, Japan Atomic Energy Agency, 2-4 Shirakata-Shirane, Tokai, Ibaraki, 319-1195, Japan, tomoyori.katsuaki@jaea.go.jp.
Katsuaki
K
Tomoyori
Katsuhiro
K
Kusaka
Taro
T
Yamada
Taro
T
Tamada
D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray / Q000295:instrumentation* / Q000379:methods*; D008565:Membrane Proteins / Q000648:ultrastructure*; D046912:Multiprotein Complexes / Q000648:ultrastructure*; D033363:Neutron Diffraction / Q000379:methods
D008565:Membrane Proteins; D046912:Multiprotein Complexes