Advanced ultra-performance liquid chromatography-photodiode array-quadrupole time-of-flight mass spectrometric methods for simultaneous screening and quantification of triterpenoids in Poria cocos.
A sensitive, precise and accurate method was developed to screen and quantify triterpenoids based on ultra-performance liquid chromatography-photodiode array-quadrupole time-of-flight mass spectrometry (UPLC-PDA-QTOF-MS). An exact neutral loss scan of 62.0004 Da (CH2O3) was used to selectively detect triterpenoids in Poria cocos, followed by a survey scan for exact masses of precursor and fragment ions of these triterpenoids. The developed method was applied to quantify seven major triterpenoids in 40 P. cocos samples of different origins within 18 min, and a total of 31 triterpenoids were unequivocally or tentatively identified. Principal component analysis of these samples showed a clear separation of three groups, and ten triterpenoids play key roles in differentiating these samples were obtained from the OPLS-DA variable influence on projection (VIP) plot and then unequivocally or tentatively identified. The developed method can be applied for rapid bitterness evaluation, quality control and authenticity establishment of P. cocos.
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
Bing
B
Xia
Shanghai University of Traditional Chinese Medicines, Shanghai 201203, China; Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China. Electronic address: zhouyan@cib.ac.cn.
Yan
Y
Zhou
Shanghai University of Traditional Chinese Medicines, Shanghai 201203, China.
Hong Sheng
HS
Tan
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
Li Sheng
LS
Ding
Shanghai University of Traditional Chinese Medicines, Shanghai 201203, China. Electronic address: xuhongxi88@gmail.com.
Hong Xi
HX
Xu
D001685:Biological Factors / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D055441:Poria / Q000737:chemistry*; D011786:Quality Control; D013729:Terpenes / Q000737:chemistry*
D001685:Biological Factors; D013729:Terpenes
Exact neutral loss scan; Poria cocos; Quadrupole time-of-flight mass spectrometry; Triterpenoid; Ultra-high performance liquid chromatography