Interactions between grape skin cell wall material and commercial enological tannins. Practical implications.
Commercial enological tannins were used to investigate the role that cell wall material plays in proanthocyanidin adsorption. Insoluble cell wall material, prepared from the skin of Vitis vinifera L. cv. Monastrell berries, was combined with solutions containing six different commercial enological tannins (proanthocyanidin-type tannins). Analysis of the proanthocyanidins in the solution, after fining with cell wall material, using phloroglucinolysis and size exclusion chromatography, provided quantitative and qualitative information on the non-adsorbed compounds. Cell wall material showed strong affinity for the proanthocyanidins, one of the commercial tannins being bound up to 61% in the experiment. Comparison of the molecular mass distribution of the commercial enological tannins in solution, before and after fining, suggested that cell walls affinity for proanthocyanidins was more related with the proanthocyanidin molecular mass than with their percentage of galloylation. These interactions may have some enological implications, especially as regards the time of commercial tannins addition to the must/wine.
Departamento de Tecnología de Alimentos, Nutrición y Bromatología, Facultad de Veterinaria, Universidad de Murcia, Campus de Espinardo, Murcia 30071, Spain.
Ana Belén
AB
Bautista-Ortín
Departamento de Tecnología de Alimentos, Nutrición y Bromatología, Facultad de Veterinaria, Universidad de Murcia, Campus de Espinardo, Murcia 30071, Spain.
Mario
M
Cano-Lechuga
Departamento de Tecnología de Alimentos, Nutrición y Bromatología, Facultad de Veterinaria, Universidad de Murcia, Campus de Espinardo, Murcia 30071, Spain.
Yolanda
Y
Ruiz-García
Departamento de Tecnología de Alimentos, Nutrición y Bromatología, Facultad de Veterinaria, Universidad de Murcia, Campus de Espinardo, Murcia 30071, Spain. Electronic address: encarnag@um.es.
Encarna
E
Gómez-Plaza
D000327:Adsorption; D002473:Cell Wall / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D008970:Molecular Weight; D010936:Plant Extracts / Q000737:chemistry*; D044945:Proanthocyanidins / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D013634:Tannins / Q000737:chemistry; D027843:Vitis / Q000737:chemistry*
D010936:Plant Extracts; D044945:Proanthocyanidins; D013634:Tannins; C013221:proanthocyanidin
Cell wall material; Grapes; Phloroglucinolysis; Size exclusion chromatography; Tannins