Oxidative Stress in Maternal Blood and Placenta From Mild Diabetic Rats.
The aim of the present study was at evaluating the effects of oxidative stress in blood and placenta of mild diabetic Wistar rats. At birth, Wistar rats received citrate buffer (nondiabetic group, n = 15) and another group received streptozotocin (100 mg/kg, subcutaneous) to induce mild diabetes (diabetic, n = 15). The glycemia of these pregnant adult female rats were evaluated at days 0, 7, 14, and 21 of pregnancy, and at term pregnancy, the blood and placental samples were collected for oxidative stress measurements. The mild diabetes caused glycemia superior to 120 mg/dL during pregnancy, increased superoxide dismutase, glutathione peroxidase, glutathione reductase activities, and malondialdehyde levels in the blood, and catalase activity in the placenta. Thus, mild diabetes increased activities of antioxidant substances aiming at defending against the exacerbated oxidative stress but were not enough. The placenta also answered to diabetic milieu and increased antioxidant defense, showing that even a mild hyperglycemia was enough to cause placental and maternal blood changes.
Graduate Program in Gynecology, Obstetrics and Mastology, Botucatu Medical School, UNESP_Univ. Estadual Paulista, Laboratory of Experimental Research on Gynecology and Obstetrics, Botucatu, São Paulo State, Brazil.
Ana Paula Machado
AP
Spada
Graduate Program in Gynecology, Obstetrics and Mastology, Botucatu Medical School, UNESP_Univ. Estadual Paulista, Laboratory of Experimental Research on Gynecology and Obstetrics, Botucatu, São Paulo State, Brazil damascenofmb@gmail.com.
Débora Cristina
DC
Damasceno
Graduate Program in Gynecology, Obstetrics and Mastology, Botucatu Medical School, UNESP_Univ. Estadual Paulista, Laboratory of Experimental Research on Gynecology and Obstetrics, Botucatu, São Paulo State, Brazil.
Yuri Karen
YK
Sinzato
Graduate Program in Gynecology, Obstetrics and Mastology, Botucatu Medical School, UNESP_Univ. Estadual Paulista, Laboratory of Experimental Research on Gynecology and Obstetrics, Botucatu, São Paulo State, Brazil Institute of Biological and Health Sciences, University Center of Araguaia, Mato Grosso Federal University (UFMT), Barra do Garças, Mato Grosso, Brazil.
Kleber Eduardo
KE
Campos
Federal University from ABC (UFABC), São Paulo, São Paulo State, Brazil.
Priscila Afonso
PA
Faria
Graduate Program in Gynecology, Obstetrics and Mastology, Botucatu Medical School, UNESP_Univ. Estadual Paulista, Laboratory of Experimental Research on Gynecology and Obstetrics, Botucatu, São Paulo State, Brazil.
Bruna
B
Dallaqua
Graduate Program in Gynecology, Obstetrics and Mastology, Botucatu Medical School, UNESP_Univ. Estadual Paulista, Laboratory of Experimental Research on Gynecology and Obstetrics, Botucatu, São Paulo State, Brazil.
Iracema Mattos Paranhos
IM
Calderon
Graduate Program in Gynecology, Obstetrics and Mastology, Botucatu Medical School, UNESP_Univ. Estadual Paulista, Laboratory of Experimental Research on Gynecology and Obstetrics, Botucatu, São Paulo State, Brazil.
Marilza Vieira Cunha
MV
Rudge
Federal University from ABC (UFABC), São Paulo, São Paulo State, Brazil.
Tiago
T
Rodrigues
diabetes; oxidative stress; placenta; rats; streptozotocin