Interferon gamma and Interleukin 10 polymorphisms in Brazilian patients with systemic lupus erythematosus.
The pathogenesis of systemic lupus erythematosus (SLE) is complex, with several susceptibility genes and environmental factors involved in its development and clinical manifestation. Currently, there is a great amount of interest in the identification of biomarkers, as cytokines, that can quantify the susceptibility of SLE, the risk of future organ involvement, and association of their changes with disease activity. To investigate the associations between polymorphisms in the gene of Interferon gamma (IFN-γ) and in the promoter of the Interleukin-10 (IL-10) gene and SLE. The polymorphisms +874 T/A (rs2430561) in the IFN-γ gene and -1082G/A (rs1800896) in the IL-10 promoter were determined in 99 SLE patients and 100 healthy controls among women Brazilian using the refractory mutation system polymerase chain reaction method. Disease activity was assessed using the SLE activity index. There were significant differences in the distribution of the genotype T/A in IFN-γ gene polymorphism (+874) (χ (2) = 7.168; P = 0.0074) and the genotype G/A in IL-10 promoter polymorphism (-1082) (χ (2) = 4.654; P = 0.0310) between the SLE and control groups. However, no association was observed between clinical features and the polymorphisms studied. This study presents preliminary evidence for association between IL-10 and IFN-γ polymorphism and SLE susceptibility, but not with clinical features in a Northeast population from Brazil.
Campus Garanhuns of Federal Institute of Pernambuco (Campus Garanhuns/IFPE), Garanhuns, Brazil.
Hildson Dornelas Angelo
HD
da Silva
Alex Paulino
AP
da Silva
Helker Albuquerque
HA
da Silva
Nadja Maria Jorge
NM
Asano
Maria de Mascena Diniz
Mde M
Maia
Paulo Roberto Eleutério
PR
de Souza
D000328:Adult; D000483:Alleles; D001938:Brazil; D016022:Case-Control Studies; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D016753:Interleukin-10 / Q000235:genetics*; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis / Q000235:genetics*; D008875:Middle Aged; D016017:Odds Ratio; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D055815:Young Adult
D016753:Interleukin-10; D007371:Interferon-gamma