Reduction of chalcogen oxyanions and generation of nanoprecipitates by the photosynthetic bacterium Rhodobacter capsulatus.
The facultative photosynthetic bacterium Rhodobacter capsulatus is characterized in its interaction with the toxic oxyanions tellurite (Te(IV)) and selenite (Se(IV)) by a highly variable level of resistance that is dependent on the growth mode making this bacterium an ideal organism for the study of the microbial interaction with chalcogens. As we have reported in the past, while the oxyanion tellurite is taken up by R. capsulatus cells via acetate permease and it is reduced to Te(0) in the cytoplasm in the form of splinter-like black intracellular deposits no clear mechanism was described for Se(0) precipitation. Here, we present the first report on the biotransformation of tellurium and selenium oxyanions into extracellular Te(0) and Se(0)nanoprecipitates (NPs) by anaerobic photosynthetically growing cultures of R. capsulatus as a function of exogenously added redox-mediator lawsone, i.e. 2-hydroxy-1,4-naphthoquinone. The NPs formation was dependent on the carbon source used for the bacterial growth and the rate of chalcogen reduction was constant at different lawsone concentrations, in line with a catalytic role for the redox mediator. X-ray diffraction (XRD) analysis demonstrated the Te(0) and Se(0) nature of the nanoparticles.
Department of Pharmacy and Biotechnology, University of Bologna, Italy. Electronic address: roberto.borghese@unibo.it.
Roberto
R
Borghese
Department of Pharmacy and Biotechnology, University of Bologna, Italy.
Chiara
C
Baccolini
Department of Pharmacy and Biotechnology, University of Bologna, Italy.
Francesco
F
Francia
Department of Chemistry G. Ciamician, University of Bologna, Italy.
Piera
P
Sabatino
Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada.
Raymond J
RJ
Turner
Department of Pharmacy and Biotechnology, University of Bologna, Italy. Electronic address: davide.zannoni@unibo.it.
Davide
D
Zannoni
D000693:Anaerobiosis; D000838:Anions / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry; D018011:Chalcogens / Q000737:chemistry* / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D009285:Naphthoquinones; D010084:Oxidation-Reduction; D010788:Photosynthesis; D016354:Rhodobacter capsulatus / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D018036:Selenium Compounds / Q000737:chemistry; D013691:Tellurium / Q000737:chemistry; D014961:X-Ray Diffraction
D000838:Anions; D001426:Bacterial Proteins; D018011:Chalcogens; D009285:Naphthoquinones; D018036:Selenium Compounds; D013691:Tellurium; C005090:lawsone
Lawsone; Nanoprecipitates; Rhodobacter capsulatus; Selenite; Tellurite