Antioxidant characterization of oak extracts combining spectrophotometric assays and chemometrics.
Antioxidant characteristics of leaves, twigs, and acorns from two Serbian oak species Quercus robur L. and Quercus petraea L. from Vojvodina province (northern Serbia) were investigated. 80% ethanol (in water) extracts were used for antiradical power (ARP) determinations against DPPH(•), (•)NO, and O2 (•-) radicals, ferric reducing antioxidant power (FRAP), total phenol, tannin, flavonoid, and proanthocyanidin contents. Permanganate reducing antioxidant capacity (PRAC) was determined using water extracts. Beside, mentioned parameters, soluble proteins, lipid peroxidation (LP), pigments and proline contents were also determined. The data of different procedures were compared and analyzed by multivariate techniques (correlation matrix calculation and principal component analysis (PCA)). PCA found that investigated organs of two different oak tree species possess similar antioxidant characteristics. The superior antioxidant characteristics showed oak leaves over twigs and acorns and seem to be promising source of antioxidants with possible use in industry and pharmacy.
Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, Serbia.
Boris M
BM
Popović
Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, Serbia.
Dubravka
D
Stajner
Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, Serbia.
Ružica
R
Zdero
Institute of Lowland Forestry and Environment, University of Novi Sad, Antona Čehova 13, 21000 Novi Sad, Serbia.
Saša
S
Orlović
Institute of Lowland Forestry and Environment, University of Novi Sad, Antona Čehova 13, 21000 Novi Sad, Serbia.
Zoran
Z
Galić
D000975:Antioxidants / Q000737:chemistry*; D002623:Chemistry Techniques, Analytical; D000431:Ethanol / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry; D005609:Free Radicals / Q000737:chemistry; D017665:Hydroxyl Radical / Q000737:chemistry; D015227:Lipid Peroxidation; D015999:Multivariate Analysis; D019800:Phenol / Q000737:chemistry; D010858:Pigmentation; D010936:Plant Extracts / Q000737:chemistry*; D025341:Principal Component Analysis; D044945:Proanthocyanidins / Q000737:chemistry; D011392:Proline / Q000737:chemistry; D029963:Quercus / Q000737:chemistry*; D013053:Spectrophotometry; D013634:Tannins / Q000737:chemistry; D014197:Trees
D000975:Antioxidants; D005419:Flavonoids; D005609:Free Radicals; D010936:Plant Extracts; D044945:Proanthocyanidins; D013634:Tannins; C013221:proanthocyanidin; D017665:Hydroxyl Radical; D019800:Phenol; D000431:Ethanol; D011392:Proline