DNA regions responsible for maintenance of Shingobium plasmid pYAN-2.
We have identified and analyzed two DNA regions responsible for stable maintenance of a plasmid in the genus Sphingomonas and Escherichia coli. A 37 bp fragment, upstream of the repA gene, is required for stable maintenance of the low-copy-number small plasmid pYAN-2 (4,687 bp) from Sphingobium yanoikuyae. It does not encode any significant protein sequence and has one direct repeat for possible secondary structures. Moreover, a 70 bp fragment, upstream of the above sequence, completely stabilized the unstable pSC101 plasmid in E. coli.
United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology.
Hiroe
H
Hayashi
Yasurou
Y
Kurusu
D020561:Alphaproteobacteria / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D018628:Gene Dosage; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial