Modulation of monocytic leukemia cell function and survival by high gradient magnetic fields and mathematical modeling studies.
The influence of spatially modulated high gradient magnetic fields on cellular functions of human THP-1 leukemia cells is studied. We demonstrate that arrays of high-gradient micrometer-sized magnets induce i) cell swelling, ii) prolonged increased ROS production, and iii) inhibit cell proliferation, and iv) elicit apoptosis of THP-1 monocytic leukemia cells in the absence of chemical or biological agents. Mathematical modeling indicates that mechanical stress exerted on the cells by high magnetic gradient forces is responsible for triggering cell swelling and formation of reactive oxygen species followed by apoptosis. We discuss physical aspects of controlling cell functions by focused magnetic gradient forces, i.e. by a noninvasive and nondestructive physical approach.
Institute of Physics AS CR, v.v.i., Prague 8, Czech Republic. Electronic address: zablot@fzu.cz.
Vitalii
V
Zablotskii
Institute of Pharmacology of Natural Products & Clinical Pharmacology, Ulm University, Ulm 89081, Germany.
Tatiana
T
Syrovets
Institute of Pharmacology of Natural Products & Clinical Pharmacology, Ulm University, Ulm 89081, Germany.
Zoe W
ZW
Schmidt
Institute of Physics AS CR, v.v.i., Prague 8, Czech Republic.
Alexandr
A
Dejneka
Institute of Pharmacology of Natural Products & Clinical Pharmacology, Ulm University, Ulm 89081, Germany. Electronic address: thomas.simmet@uni-ulm.de.
Thomas
T
Simmet
D017209:Apoptosis; D045744:Cell Line, Tumor; D006801:Humans; D007938:Leukemia / Q000378:metabolism / Q000473:pathology*; D008280:Magnetics; D008962:Models, Theoretical; D009000:Monocytes / Q000378:metabolism / Q000473:pathology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013314:Stress, Mechanical
D017382:Reactive Oxygen Species
Apoptosis; Cell proliferation; Leukemia; Magnetic field; Modeling; Reactive oxygen species