Effect of nitrogen-starvation, light intensity and iron on triacylglyceride/carbohydrate production and fatty acid profile of Neochloris oleoabundans HK-129 by a two-stage process.
Triacylglyceride (TAG) and carbohydrate are potential feedstock for biofuels production. In this study, a two-stage process was applied for enhancing TAG/carbohydrate production in the selected microalgae - Neochloris oleoabundans HK-129. In stage I, effects of nitrogen, light intensity and iron on cell growth were investigated, and the highest biomass productivity of 292.83±5.83mg/L/d was achieved. In stage II, different nitrogen-starvation periods, light intensities and iron concentrations were employed to trigger accumulation of TAG and carbohydrate. The culture under 2-day N-starvation, 200μmol/m(2)/s light intensity and 0.037mM Fe(3+) concentration produced the maximum TAG and carbohydrate productivity of 51.58mg/L/d and 90.70mg/L/d, respectively. Nitrogen starvation period and light intensity had marked effects on TAG/carbohydrate accumulation and fatty acids profile, compared to iron concentration. The microalgal lipid was mainly composed of C16/C18 fatty acids (90.02%), saturated fatty acids (29.82%), and monounsaturated fatty acids (32.67%), which is suitable for biodiesel synthesis.
Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu 610064, Sichuan, China.
Xian
X
Sun
Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu 610064, Sichuan, China.
Yu
Y
Cao
Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu 610064, Sichuan, China.
Hui
H
Xu
Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu 610064, Sichuan, China.
Yan
Y
Liu
Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu 610064, Sichuan, China.
Jianrui
J
Sun
Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu 610064, Sichuan, China.
Dairong
D
Qiao
Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu 610064, Sichuan, China. Electronic address: geneium@scu.edu.cn.
Yi
Y
Cao
D000704:Analysis of Variance; D002241:Carbohydrates / Q000096:biosynthesis*; D018929:Cell Culture Techniques; D000460:Chlorophyta / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D005227:Fatty Acids / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods; D007501:Iron / Q000494:pharmacology*; D008027:Light; D009584:Nitrogen / Q000172:deficiency*; D010078:Oxazines; D014280:Triglycerides / Q000096:biosynthesis*
D002241:Carbohydrates; D005227:Fatty Acids; D010078:Oxazines; D014280:Triglycerides; D007501:Iron; D009584:Nitrogen; C044808:nile red
Carbohydrate; Microalgae; Nitrogen starvation; Triacylglycerol; Two-stage process