Effects of palmitoylation on the diffusional movement of BKCa channels in live cells.
BKCa channels are palmitoylated at a cluster of cysteine residues within the cytosolic linker connecting the 1st and 2nd transmembrane domains, and this lipid modification affects their surface expression. To verify the effects of palmitoylation on the diffusional dynamics of BKCa channels, we investigated their lateral movement. Compared to wild-type channels, the movement of mutant palmitoylation-deficient channels was much less confined and close to random. The diffusion of the mutant channel was also much faster than that of the wild type. Thus, the lateral movement of BKCa channels is greatly influenced by palmitoylation.
School of Life Sciences, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea.
Sulgi
S
Kim
School of Life Sciences, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea; National Leading Research Laboratory, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea; Cell Dynamics Research Center, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea.
Byoung-Cheol
BC
Lee
School of Life Sciences, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea; National Leading Research Laboratory, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea; Cell Dynamics Research Center, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea.
A-Ram
AR
Lee
School of Life Sciences, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea.
Sehoon
S
Won
School of Life Sciences, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea; National Leading Research Laboratory, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea; Cell Dynamics Research Center, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea. Electronic address: cspark@gist.ac.kr.
Chul-Seung
CS
Park
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D017124:Conserved Sequence; D004058:Diffusion; D051037:Large-Conductance Calcium-Activated Potassium Channel alpha Subunits; D054878:Lipoylation; D021962:Membrane Microdomains / Q000378:metabolism; D015221:Potassium Channels / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D021381:Protein Transport; D051381:Rats
C494842:Kcnma1 protein, rat; D051037:Large-Conductance Calcium-Activated Potassium Channel alpha Subunits; D015221:Potassium Channels
BK(Ca) channel; Lateral diffusion; Lipid raft; Palmitoylation; Quantum dot; Single molecule