High-resolution visualization of mouse cardiac microvasculature using optical histology.
Cardiovascular disease typically is associated with dysfunction of the coronary vasculature and microvasculature. The study of cardiovascular disease typically involves imaging of the large coronary vessels and quantification of cardiac blood perfusion. These methods, however, are not well suited for imaging of the cardiac microvasculature. We used the optical histology method, which combines chemical optical clearing and optical imaging, to create high-resolution, wide-field maps of the cardiac microvasculature in ventral slices of mouse heart. We have demonstrated the ability of the optical histology method to enable wide-field visualization of the cardiac microvasculature in high-resolution and anticipate that optical histology may have significant impact in studying cardiovascular disease.
Beckman Laser Institute, University of California, Irvine, 1002 Health Sciences Road East, Irvine, CA, USA.
Austin J
AJ
Moy
Beckman Laser Institute, University of California, Irvine, 1002 Health Sciences Road East, Irvine, CA, USA.
Patrick C
PC
Lo
Beckman Laser Institute, University of California, Irvine, 1002 Health Sciences Road East, Irvine, CA, USA.
Bernard
B
Choi
(000.1430) Biology and medicine; (170.0170) Medical optics and biotechnology; (170.1790) Confocal microscopy; (170.3880) Medical and biological imaging; (170.6930) Tissue