Gene and miRNA expression profiles of hematopoietic progenitor cells vary depending on their origin.
Hematopoietic progenitor cells (HPCs) from granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood (G-PB), bone marrow (BM), or umbilical cord blood (CB) have differing biological properties and differing kinetics of engraftment post-transplantation, which might be explained, at least in part, by differing gene and miRNA expression patterns. To assess the differences in gene and miRNA expression, we analyzed whole genome expression profiles as well as the expression of 384 miRNAs in CD34(+) cells isolated from 18 healthy individuals (6 individuals per subtype of HPC source). We identified 43 genes and 36 miRNAs differentially expressed in the various CD34(+) cell sources. We observed that CD34(+) cells from CB and BM showed similar gene and miRNA expression profiles, whereas CD34(+) cells from G-PB had a very different expression pattern. Remarkably, 20 of the differentially expressed genes are targets of the differentially expressed miRNAs. Of note, the majority of genes differentially expressed in CD34(+) cells from G-PB are involved in cell cycle regulation, promoting the process of proliferation, survival, hematopoiesis, and cell signaling, and are targets of overexpressed and underexpressed miRNAs in CD34(+) cells from the same source. These data suggest significant differences in gene and miRNA expression among the various HPC sources used in transplantation. We hypothesize that the differentially expressed genes and miRNAs involved in cell cycle and proliferation might explain the differing kinetics of engraftment observed after transplantation of hematopoietic stem cells obtained from these different sources.
Department of Hematology, University Hospital Virgen del Rocio/Institute of Biomedicine of Seville (IBIS)/CSIC/University of Seville. Electronic address: abaez-ibis@us.es.
Alicia
A
Báez
Department of Hematology/Hospital Clinic/IDIBAPS and Institute of Research Josep Carreras/University of Barcelona.
Beatriz
B
Martín-Antonio
Department of Hematology, University Hospital Virgen del Rocio/Institute of Biomedicine of Seville (IBIS)/CSIC/University of Seville.
José I
JI
Piruat
Department of Hematology, University Hospital Virgen del Rocio/Institute of Biomedicine of Seville (IBIS)/CSIC/University of Seville.
Maria Victoria
MV
Barbado
Department of Hematology, University Hospital Virgen del Rocio/Institute of Biomedicine of Seville (IBIS)/CSIC/University of Seville.
Concepción
C
Prats
Department of Hematology, University Hospital Virgen del Rocio/Institute of Biomedicine of Seville (IBIS)/CSIC/University of Seville.
Isabel
I
Álvarez-Laderas
Department of Hematology, University Hospital Virgen del Rocio/Institute of Biomedicine of Seville (IBIS)/CSIC/University of Seville.
Magdalena
M
Carmona
Department of Hematology, University Hospital Virgen del Rocio/Institute of Biomedicine of Seville (IBIS)/CSIC/University of Seville.
José Antonio
JA
Pérez-Simón
Department of Hematology/Hospital Clinic/IDIBAPS and Institute of Research Josep Carreras/University of Barcelona.
Álvaro
Á
Urbano-Ispizua
D018952:Antigens, CD34 / Q000235:genetics / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002453:Cell Cycle / Q000235:genetics; D049109:Cell Proliferation; D005312:Fetal Blood / Q000166:cytology / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015894:Genome, Human; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction
D018952:Antigens, CD34; D035683:MicroRNAs
Gene expression; Hematopoietic progenitor cell; miRNA