Antibacterial effects of natural tenderizing enzymes on different strains of Escherichia coli O157:H7 and Listeria monocytogenes on beef.
This study determined the efficacy of actinidin and papain on reducing Listeria monocytogenes and three mixed strains of Escherichia coli O157:H7 populations on beef. The average reduction of E. coli O157:H7 was greater than that of L. monocytogenes and higher concentrations of either protease yielded greater reduction in bacterial populations. For instance, actinidin at 700 mg/ml significantly (p≤0.05) reduced the population of L. monocytogenes by 1.49 log cfu/ml meat rinse after 3h at 25 & 35 °C, and by 1.45 log cfu/ml rinse after 24h at 5 °C, while the same actinidin concentration significantly reduced the populations of three mixed strains of E. coli O157:H7 by 1.81 log cfu/ml rinse after 3h at 25 & 35 °C, and 1.94 log cfu/ml rinse after 24h at 5 °C. These findings suggest that, in addition to improving the sensory attributes of beef, proteolytic enzymes can enhance meat safety when stored at suitable temperatures.
Food, Nutrition and Packaging Sciences Department, Clemson University, Clemson, SC, United States.
Hanan
H
Eshamah
Food, Nutrition and Packaging Sciences Department, Clemson University, Clemson, SC, United States.
Inyee
I
Han
Department of Food Hygiene, Faculty of Veterinary Medicine, University of Tripoli, Tripoli, Libya.
Hesham
H
Naas
Food, Nutrition and Packaging Sciences Department, Clemson University, Clemson, SC, United States.
James
J
Acton
Food, Nutrition and Packaging Sciences Department, Clemson University, Clemson, SC, United States. Electronic address: pdawson@clemson.edu.
Paul
P
Dawson
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002417:Cattle; D015169:Colony Count, Microbial; D003546:Cysteine Endopeptidases / Q000494:pharmacology*; D019453:Escherichia coli O157 / Q000187:drug effects*; D005516:Food Microbiology; D006801:Humans; D008089:Listeria monocytogenes / Q000187:drug effects*; D008460:Meat / Q000382:microbiology*; D010206:Papain / Q000494:pharmacology*; D013649:Taste
D000900:Anti-Bacterial Agents; D003546:Cysteine Endopeptidases; C018745:actinidain; D010206:Papain
Actinidin; Escherichia coli O157:H7; Listeria monocytogenes; Papain; Proteolytic enzymes