Relocation of the NIb gene in the tobacco etch potyvirus genome.
Potyviruses express most of their proteins from a long open reading frame that is translated into a large polyprotein processed by three viral proteases. To understand the constraints on potyvirus genome organization, we relocated the viral RNA-dependent RNA polymerase (NIb) cistron to all possible intercistronic positions of the Tobacco etch virus (TEV) polyprotein. Only viruses with NIb at the amino terminus of the polyprotein or in between P1 and HC-Pro were viable in tobacco plants.
Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-Universidad Politécnica de Valencia, Valencia, Spain.
Eszter
E
Majer
Zaira
Z
Salvador
Mark P
MP
Zwart
Anouk
A
Willemsen
Santiago F
SF
Elena
José-Antonio
JA
Daròs
D000595:Amino Acid Sequence; D012321:DNA-Directed RNA Polymerases / Q000235:genetics* / Q000378:metabolism; D023061:Gene Order; D016679:Genome, Viral; D010935:Plant Diseases / Q000821:virology*; D017800:Potyvirus / Q000201:enzymology* / Q000235:genetics* / Q000502:physiology; D014026:Nicotiana / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases