Development and validation of a reversed-phase high-performance liquid chromatography method for routine identification and purity assessment of high-purity steviol glycoside sweeteners.
The widespread application of stevia-based sweeteners in food products has resulted in the need for reliable analytical methods for measuring the purity and identity of high-purity steviol glycoside ingredients. The objective of this research was to develop and validate a new reversed-phase separation method capable of separating and quantifying nine steviol glycosides present in typical high-purity stevia extract ingredients. Results of the study established the linearity of the method at a correlation factor of 1.000 for the two major components and other minor components of this food ingredient. Method accuracy values were in the range of 99.1-100.9%. The percent relative standard deviation for six independent assay determinations was 1.0%. The method was determined to be robust for minor changes in column temperature, initial acetonitrile content, flow rate, and wavelength. The validated high-performance liquid chromatography method was found to be suitable to be included by USP as a Food Chemicals Codex compendial standard for steviol glycosides.
Research and Development and Food Standards, United States Pharmacopeial Convention , 12601 Twinbrook Parkway, Rockville, Maryland 20852, United States.
Tsion
T
Bililign
Jeffrey C
JC
Moore
Shane
S
Tan
Allan T
AT
Leeks
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D045786:Diterpenes, Kaurane / Q000032:analysis*; D004355:Drug Stability; D063427:Food Quality; D006027:Glycosides / Q000032:analysis*; D013549:Sweetening Agents
D045786:Diterpenes, Kaurane; D006027:Glycosides; D013549:Sweetening Agents; C012042:steviol; C025474:rebaudioside A