A computational model of clavicle bone formation: a mechano-biochemical hypothesis.
Clavicle development arises from mesenchymal cells condensed as a cord extending from the acromion towards the sternal primordium. First two primary ossification centers form, extending to develop the body of the clavicle through intramembranous ossification. However, at its ends this same bone also displays endochondral ossification. So how can the clavicle be formed by both types of ossification? Developmental events associated with clavicle formation have mainly used histological studies as supporting evidence. Nonetheless, mechanisms of biological events such as molecular and mechanical effects remain to be determined. The objective of this work was to provide a mathematical explanation of embryological events based on two serial phases: first formation of an ossified matrix by intramembranous ossification based on three factors: systemic, local biochemical, and mechanical factors. After this initial phase expansion of the ossified matrix follows with mesenchymal cell differentiation into chondrocytes for posterior endochondral ossification. Our model provides strong evidence for clavicle formation integrating molecules and mechanical stimuli through partial differentiation equations using finite element analysis.
Departament of Mechanical and Mechatronics Engineering, Numerical Methods and Modeling Group Research (GNUM), Instituto de Biotecnología, Universidad Nacional de Colombia, Bogotá, Colombia; Group of Mechanobiology of Organs and Biological Tissues (Mech+Biol_UN), Biomimetics Laboratory, Instituto de Biotecnología, Universidad Nacional de Colombia, Bogotá, Colombia. Electronic address: dagarzona@unal.edu.co.
Diego A
DA
Garzon-Alvarado
Departament of Mechanical and Mechatronics Engineering, Numerical Methods and Modeling Group Research (GNUM), Instituto de Biotecnología, Universidad Nacional de Colombia, Bogotá, Colombia. Electronic address: mlgutierrezgo@unal.edu.co.
María Lucía
ML
Gutiérrez
Departament of Mechanical and Mechatronics Engineering, Numerical Methods and Modeling Group Research (GNUM), Instituto de Biotecnología, Universidad Nacional de Colombia, Bogotá, Colombia; Department of Orthopaedic Surgery, School of Medicine, Universidad Nacional de Colombia, Bogotá, Colombia; Group of Mechanobiology of Organs and Biological Tissues (Mech+Biol_UN), Biomimetics Laboratory, Instituto de Biotecnología, Universidad Nacional de Colombia, Bogotá, Colombia. Electronic address: lfcalixtob@unal.edu.co.
Luis Fernando
LF
Calixto
D001696:Biomechanical Phenomena; D002968:Clavicle / Q000196:embryology*; D006801:Humans; D008954:Models, Biological; D010012:Osteogenesis / Q000502:physiology*
Clavicle formation; Finite elements analysis; Reaction diffusion; Turing pattern formation