The presence of an air-water interface affects formation and elongation of α-Synuclein fibrils.
The aggregation of human α-Synuclein (α-Syn) into amyloid fibrils is related to the onset of multiple diseases termed synucleinopathies. Substantial evidence suggests that hydrophobic-hydrophilic interfaces promote the aggregation of amyloidogenic proteins and peptides in vitro. In this work the effect of the air-water interface (AWI) on α-Syn aggregation is investigated by means of thioflavin T binding measurements, dynamic light scattering, size-exclusion chromatography, electron microscopy, and atomic force microscopy. Measurements were performed with the monomeric protein alone or together with preformed seeds. In presence of the AWI, α-Syn aggregates readily into amyloid fibrils that remain adsorbed to the AWI. Instead, when the AWI is removed from the samples by replacing it with a solid-liquid interface, the interfacial aggregation of monomeric α-Syn is greatly reduced and no significant increase in ThT fluorescence is detected in the bulk, even at 900 μM concentration. Bulk aggregation is observed only when a sufficient amount of preformed seeds is added, and the initial slope of the kinetics scales with the amount of seeds as expected for first order kinetics. By contrast, in seeded experiments with the AWI, the initial slope is one order of magnitude lower and secondary nucleation pathways appear instead to be dominant. Thus, interfaces play multiple roles in the aggregation of α-Syn, influencing primary nucleation, aggregate elongation, and secondary nucleation processes. Interfacial effects must therefore be taken into account to achieve a complete understanding of protein aggregation events in vitro as well as in vivo.
Laboratory of Physical Chemistry, Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology Zurich , Wolfgang-Pauli-Str. 10, 8093 Zurich, Switzerland.
Silvia
S
Campioni
Guillaume
G
Carret
Sophia
S
Jordens
Lucrèce
L
Nicoud
Raffaele
R
Mezzenga
Roland
R
Riek
D000388:Air; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D055503:Protein Multimerization / Q000187:drug effects*; D017433:Protein Structure, Secondary / Q000187:drug effects; D013499:Surface Properties; D014867:Water / Q000494:pharmacology*; D051844:alpha-Synuclein / Q000737:chemistry*
D051844:alpha-Synuclein; D014867:Water