Oxylipin diversity in the diatom family Leptocylindraceae reveals DHA derivatives in marine diatoms.
Marine planktonic organisms, such as diatoms, are prospective sources of novel bioactive metabolites. Oxygenated derivatives of fatty acids, generally referred to as oxylipins, in diatoms comprise a highly diverse and complex family of secondary metabolites. These molecules have recently been implicated in several biological processes including intra- and inter-cellular signaling as well as in defense against biotic stressors and grazers. Here, we analyze the production and diversity of C20 and C22 non-volatile oxylipins in five species of the family Leptocylindraceae, which constitute a basal clade in the diatom phylogeny. We report the presence of species-specific lipoxygenase activity and oxylipin patterns, providing the first demonstration of enzymatic production of docosahexaenoic acid derivatives in marine diatoms. The differences observed in lipoxygenase pathways among the species investigated broadly reflected the relationships observed with phylogenetic markers, thus providing functional support to the taxonomic diversity of the individual species.
Stazione Zoologica Anton Dohrn, Villa Comunale, Naples 80121, Italy. depac.n@gmail.com.
Deepak
D
Nanjappa
Stazione Zoologica Anton Dohrn, Villa Comunale, Naples 80121, Italy. giuliana.dippolito@icb.cnr.it.
Giuliana
G
d'Ippolito
Stazione Zoologica Anton Dohrn, Villa Comunale, Naples 80121, Italy. carmen.gallo@icb.cnr.it.
Carmela
C
Gallo
Stazione Zoologica Anton Dohrn, Villa Comunale, Naples 80121, Italy. zingone@szn.it.
Adriana
A
Zingone
Stazione Zoologica Anton Dohrn, Villa Comunale, Naples 80121, Italy. angelo.fontana@icb.cnr.it.
Angelo
A
Fontana
D004247:DNA / Q000235:genetics; D017377:Diatoms / Q000737:chemistry* / Q000235:genetics; D004281:Docosahexaenoic Acids / Q000737:chemistry*; D016013:Likelihood Functions; D058945:Lipoxygenases / Q000378:metabolism; D008968:Molecular Conformation; D054883:Oxylipins / Q000737:chemistry* / Q000302:isolation & purification; D010802:Phylogeny; D011506:Proteins / Q000737:chemistry; D015398:Signal Transduction / Q000502:physiology; D013045:Species Specificity; D013237:Stereoisomerism
D054883:Oxylipins; D011506:Proteins; D004281:Docosahexaenoic Acids; D004247:DNA; D058945:Lipoxygenases