[miR-124a promotes neurite outgrowth by inhibiting iASPP expression].
OBJECTIVE
To investigate the role of iASPP as the target gene of miR-124a in neural development.


METHODS
Using the online bioinformatical tool (TargetScan) and by reviewing the relevant studies, we selected iASPP as the candidate target gene of miR-124a involved in early-stage neuronal differentiation. Luciferase reporter assay was used to verify the candidate gene. We transfected M17 cells with a miR-124a overexpression plasmid and detected the changes in the protein expression of iASPP using Western blotting. With retinoic acid-induced M17 cells as the neuronal differentiation model, the role of iASPP in early-stage neuronal differentiation was investigated by gene overexpression and gene interference techniques.


RESULTS
miR-124a inhibited the expression of iASPP in M17 cells by interacting with the 3'UTR of iASPP gene. miR-124a promoted neurite outgrowth of the cells, which was blocked by iASPP overexpression.


CONCLUSIONS
miR-124a promotes neurite outgrowth of M17 cells by inhibiting iASPP expression.
Department of Neurobiology, Southern Medical University, Guangzhou 510515, China. E-mail: natasha0612@163.com.
Lifang
L
Lin
Xi
X
Gu
Shuhu
S
Liu
Xuemin
X
Wang
D020413:3' Untranslated Regions; D015870:Gene Expression; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics*; D016501:Neurites / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
D020413:3' Untranslated Regions; D047908:Intracellular Signaling Peptides and Proteins; C509207:MIRN124 microRNA, human; D035683:MicroRNAs; C119612:PPP1R13L protein, human; D012097:Repressor Proteins