Electrostatic spray ionization mass spectrometry imaging.
Imaging samples on a surface by mass spectrometry (MS) requires the combination of MS detection with a scanning mode that enables localized desorption and ionization and/or detection of sample analytes with good spatial resolution. We have developed a new mass spectrometry imaging (MSI) method based on electrostatic spray ionization. It works under ambient conditions and can be applied to a wide range of molecules providing quantitative MS analysis even in the presence of salts in excess. 2D MS images of protein and peptide spots, inkjet-printed black dye patterns, and cells were obtained. The presented novel ambient ionization mass spectrometry imaging method can find many applications in analytical and bioanalytical chemistry.
Laboratoire d'Electrochimie Physique et Analytique, Ecole Polytechnique Fédérale de Lausanne , Station 6, CH-1015 Lausanne, Switzerland.
Liang
L
Qiao
Elena
E
Tobolkina
Andreas
A
Lesch
Alexandra
A
Bondarenko
Xiaoqin
X
Zhong
Baohong
B
Liu
Horst
H
Pick
Horst
H
Vogel
Hubert H
HH
Girault
D045744:Cell Line, Tumor; D006801:Humans; D011327:Printing / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D055672:Static Electricity