Acrylamide-induced apoptosis in rat primary astrocytes and human astrocytoma cell lines.
This study aimed to evaluate the acrylamide (ACR)-induced apoptotic effects on rat primary astrocytes and three human astrocytoma-derived cell lines (U-1240 MG, U-87 MG, and U-251 MG). As determined through the MTT assay, treatment with 1 and 2 mM ACR for 24-72 h resulted in decreased cell viability in all cells. Decreases in cell viability could be blocked in all cells with the exception of U-251 MG cells by Z-DEVD FMK. ACR-induced dose-dependent apoptotic effects were also demonstrated by increases in the sub-G1 phase cell population in all cells. The decreased expressions of pro-caspase 3, 8, and 9 and the interruption of the mitochondrial membrane potential were observed in all cells. Exposure to 2 mM ACR for 48 h resulted in increased Bax/Bcl-2 ratios in primary astrocytes and U-87 MG cells, whereas the overexpression of Bcl-2 was observed in U-1240 MG and U-251 MG cells. The ACR-induced increases in the levels of p53 and pp53 in primary astrocytes could be attenuated by caffeine. These results suggest the existence of a common apoptotic pathway among all cell types and that U-87 MG cells may be a suitable substitute in vitro model for primary astrocytes in future studies on ACR-induced neurotoxicity.
School of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei 106, Taiwan.
Jiann-Gwu
JG
Lee
School of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei 106, Taiwan.
Yan-Shiu
YS
Wang
School of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei 106, Taiwan. Electronic address: chouchin@ntu.edu.tw.
Chin-Cheng
CC
Chou
D020106:Acrylamide / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001253:Astrocytes / Q000166:cytology* / Q000187:drug effects*; D001254:Astrocytoma; D045744:Cell Line, Tumor; D002470:Cell Survival; D004785:Environmental Pollutants / Q000633:toxicity; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051381:Rats
D004785:Environmental Pollutants; D020106:Acrylamide
Acrylamide; Apoptosis; Astrocyte; Mitochondria; Neurotoxicity