Nanoparticle coated paper-based chemiluminescence device for the determination of L-cysteine.
A laminated paper-based analytical device (LPAD) combined with gold nanoparticles (AuNPs) catalyzed luminol chemiluminescence (CL) system for the determination of L-cysteine (L-cys) was presented here. It is based on the principle that L-cys can greatly inhibit CL signal of AuNPs-luminol-H2O2 system. The paper-based device was fabricated by a low-cost cutting method, followed by lamination with two polyester films. This approach of cutting/lamination to fabricate LPAD is very similar to making a lamination picture. A good linear relationship was obtained between the CL intensity and the concentrations of L-cys in the range from 1.0×10(-8) M to 1.0×10(-6) M with a detection limit at 8.2×10(-10) M (S/N=3). This study shows the successful integration of the LPAD and the CL method. It will be of interest for use in areas such as disease diagnosis in the future.
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China. Electronic address: weiliu@126.com.
Wei
W
Liu
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.
Jing
J
Luo
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.
Yumei
Y
Guo
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.
Juan
J
Kou
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.
Baoxin
B
Li
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.
Zhujun
Z
Zhang
D003545:Cysteine / Q000032:analysis*; D004867:Equipment Design; D006046:Gold / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D057230:Limit of Detection; D049449:Luminescence; D008163:Luminescent Measurements / Q000295:instrumentation*; D008165:Luminol / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D010209:Paper
D008165:Luminol; D006046:Gold; D006861:Hydrogen Peroxide; D003545:Cysteine
Chemiluminescence; Gold nanoparticle; Laminated paper-based analytical device; l-cysteine