Technology and techniques for microbial ecology via DNA sequencing.
High-throughput sequencing technology, coupled with the use of conserved marker genes, has allowed for the understanding of communities of microbes (both culturable and unculturable) as well as their phylogenetic placement. The recent explosion of sequencing data prompted the development of software that could process the vast amount of data generated and phylogenetically differentiate groups of samples. Host-associated microbial studies have revealed that microbes are highly varied between individuals and fluctuate within an individual. Large-scale studies are being undertaken that include collection of extensive environmental data to help uncover the forces that shape microbial communities.
1 Molecular, Cellular and Developmental Biology.
William A
WA
Walters
Rob
R
Knight
D004269:DNA, Bacterial / Q000032:analysis*; D004275:DNA, Ribosomal / Q000032:analysis*; D006801:Humans; D054892:Metagenome / Q000235:genetics*; D064307:Microbiota / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods*; D012984:Software
D004269:DNA, Bacterial; D004275:DNA, Ribosomal