Microvascular platforms for the study of platelet-vessel wall interactions.
Platelets interact with the endothelium to regulate vascular integrity and barrier function, mediate inflammation and immune response, and prevent and arrest hemorrhage. In this review, we describe existing tools to study the flow-dependent interactions of platelets with the vessel wall. We also discuss our work on building engineered microvessels to study the roles of platelets on endothelial barrier function, endothelial sprouting, and thrombus formation on both quiescent and stimulated endothelium. In particular, we will show the advantage of using a cell-remodelable system in the studies of platelet-vessel wall interactions.
Department of Bioengineering, University of Washington, Seattle, WA, USA; Center for Cardiovascular Biology, University of Washington, Seattle, WA, USA; Institute of Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA, USA. Electronic address: yingzy@uw.edu.
Ying
Y
Zheng
Puget Sound Blood Center Research Institute, Seattle, WA, USA.
Junmei
J
Chen
Puget Sound Blood Center Research Institute, Seattle, WA, USA; Department of Medicine, University of Washington, Seattle, WA, USA; Department of Biochemistry, University of Washington, Seattle, WA, USA.
José A
JA
López
D000818:Animals; D001792:Blood Platelets / Q000166:cytology / Q000502:physiology*; D042783:Endothelial Cells / Q000166:cytology; D004727:Endothelium / Q000502:physiology*; D006801:Humans