Genetic polymorphism characteristics of Brucella canis isolated in China.
In China, brucellosis is an endemic disease typically caused by Brucella melitensis infection (biovars 1 and 3). Brucella canis infection in dogs has not traditionally recognized as a major problem. In recent years however, brucellosis resulting from Brucella canis infection has also been reported, suggesting that infections from this species may be increasing. Data concerning the epidemiology of brucellosis resulting from Brucella canis infection is limited. Therefore, the purpose of this study was to assess the diversity among Chinese Brucella canis strains for epidemiological purposes. First, we employed a 16-marker VNTR assay (Brucella MLVA-16) to assess the diversity and epidemiological relationship of 29 Brucella canis isolates from diverse locations throughout China with 38 isolates from other countries. MLVA-16 analysis separated the 67 Brucella canis isolates into 57 genotypes that grouped into five clusters with genetic similarity coefficients ranging from 67.73 to 100%. Moreover, this analysis revealed a new genotype (2-3-9-11-3-1-5-1:118), which was present in two isolates recovered from Guangxi in 1986 and 1987. Second, multiplex PCR and sequencing analysis were used to determine whether the 29 Chinese Brucella canis isolates had the characteristic BMEI1435 gene deletion. Only two isolates had this deletion. Third, amplification of the omp25 gene revealed that 26 isolates from China had a T545C mutation. Collectively, this study reveals that considerable diversity exists among Brucella canis isolates in China and provides resources for studying the genetic variation and microevolution of Brucella.
Laboratory of Zoonoses, China Animal Health and Epidemiology Center, MOA, Qingdao, China.
Dongdong
D
Di
State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Beijing, China.
Buyun
B
Cui
Laboratory of Endemic and Parasitic Diseases Control and Prevention, Hangzhou Center for Disease Control and Prevention, Hangzhou, China.
Heng
H
Wang
State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Beijing, China.
Hongyan
H
Zhao
State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Beijing, China.
Dongri
D
Piao
Laboratory of Zoonoses, China Animal Health and Epidemiology Center, MOA, Qingdao, China.
Lili
L
Tian
State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Beijing, China.
Guozhong
G
Tian
Laboratory of Zoonoses, China Animal Health and Epidemiology Center, MOA, Qingdao, China.
Jingli
J
Kang
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
Xiang
X
Mao
Jining District Animal Disease Control Center, Jining District Agriculture and Animal Husbandry Bureau, Wulanchabu, China.
Xiaojun
X
Zhang
Laboratory of Zoonoses, China Animal Health and Epidemiology Center, MOA, Qingdao, China.
Pengfei
P
Du
Laboratory of Zoonoses, China Animal Health and Epidemiology Center, MOA, Qingdao, China.
Lin
L
Zhu
Liaoning Center for Disease Control and Prevention, Shenyang, China.
Zhuo
Z
Zhao
Liaoning Center for Disease Control and Prevention, Shenyang, China.
Lingling
L
Mao
Liaoning Center for Disease Control and Prevention, Shenyang, China.
Wenqing
W
Yao
College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, China.
Pingyuan
P
Guan
Laboratory of Zoonoses, China Animal Health and Epidemiology Center, MOA, Qingdao, China.
Weixing
W
Fan
State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Beijing, China.
Hai
H
Jiang
D000818:Animals; D041002:Brucella canis / Q000145:classification / Q000235:genetics*; D002006:Brucellosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D002681:China / Q000453:epidemiology; D004196:Disease Outbreaks; D004283:Dog Diseases / Q000453:epidemiology* / Q000382:microbiology; D004285:Dogs; D005784:Gene Amplification; D005798:Genes, Bacterial; D005819:Genetic Markers; D005838:Genotype; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide
D005819:Genetic Markers