Effects of C358A polymorphism of the endocannabinoid degrading enzyme fatty acid amide hydrolase (FAAH) on weight loss, adipocytokines levels, and insulin resistance after a high polyunsaturated fat diet in obese patients.
OBJECTIVE
The C385A polymorphism of FAAH gene (rs324420C>A) has been associated with obesity. We investigate the role of this polymorphism on anthropometric and insulin resistance responses to a high polyunsaturated fat hypocaloric diet.


METHODS
Obese individuals (no.=99) were assessed at baseline and after 3 months of a high polyunsaturated fat hypocaloric diet.


RESULTS
Seventy-one patients (71.7%) had the genotype C385C and 28 (28.3%) patients had the C385A (26 patients, 26.3%) or A358A (2 patients, 2.0%) (A allele carriers group) genotype. In A allele carriers and after dietary intervention, total cholesterol (-16.3 ± 37.4 mg/dl) and LDL-cholesterol (-12.9 ± 6.5 mg/dl) levels decreased. In subjects with C385C genotype, the decreases were significant in total cholesterol (-12.3 ± 27.4 mg/dl), LDL-cholesterol (-7.5 ± 20.5 mg/dl), insulin (-2.2 ± 6.2 mUI/l), and homeostasis model assessment of insulin resistance (HOMA-R) (-0.79 ± 1.15 units) levels. The weight loss was similar in both genotype groups (-4.1 ± 3.8 kg vs -4.2 ± 3.2 kg). Only leptin levels had a significant similar decrease in both genotypes.


CONCLUSIONS
Subjects with C385C genotype of the FAAH showed an improvement on insulin and HOMA-R levels with a high polyunsaturated fat hypocaloric diet after weight loss during 3 months.
Institute of Endocrinology and Nutrition, Medicine School and Unit of Investigation, Hospital Rio Hortega, University of Valladolid, C/Los perales 16, Simancas 47130, Valladolid, Spain. dadluis@yahoo.es.
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de Luis
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Izaola
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Aller
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de La Fuente
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Pacheco
D054392:Adipokines / Q000097:blood; D000581:Amidohydrolases / Q000235:genetics*; D002784:Cholesterol; D059305:Diet, High-Fat; D004038:Diet, Reducing; D063388:Endocannabinoids / Q000378:metabolism; D005260:Female; D006801:Humans; D007328:Insulin; D007333:Insulin Resistance / Q000502:physiology; D008297:Male; D009765:Obesity / Q000235:genetics; D011110:Polymorphism, Genetic; D011446:Prospective Studies; D015431:Weight Loss / Q000502:physiology*; D000099252:Fatty Acid Amide Hydrolases
D054392:Adipokines; D000581:Amidohydrolases; D002784:Cholesterol; D063388:Endocannabinoids; D007328:Insulin; D000099252:Fatty Acid Amide Hydrolases