A facile approach for imprinting protein on the surface of multi-walled carbon nanotubes.
This study describes a green, facile and low cost approach for imprinting protein on the surface of multi-walled carbon nanotubes (MWNTs) using papain as the template, dopamine as the functional monomer. By simply mixing MWNTs, dopamine, template protein in weak alkaline aqueous solution, a thin adherent polydopamine (PDA) film imprinted with protein was spontaneously obtained on the surface of MWNTs to produce the imprinted nanomaterials (MWNTs@MIPs). The obtained MWNTs@MIPs were characterized with Fourier transform infrared spectrometer (FT-IR), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The adsorption process of the MWNTs@MIPs towards template protein was investigated in detail. The effects of the concentration of the monomer and template, polymerization time, extraction process were optimized. The prepared MWNTs@MIPs show fast binding kinetics, high binding capacity and acceptable specific recognition behavior towards template proteins. Furthermore, the stability and regeneration were also investigated, which indicated that the MWNTs@MIPs had good reusability. The good recognizing behavior coupled to the low cost and facile one-step preparation make the MWNTs@MIPs attractive for separation and specific protein recognition.
The Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China.
Ren
R
Liu
The Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China.
Mo
M
Sha
The Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China.
Sisi
S
Jiang
The Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China; State Key Laboratory of Molecular Engineering of Polymers (Fudan University), Shanghai 200433, China. Electronic address: jingluo19801007@126.com.
Jing
J
Luo
The Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address: lxy@jiangnan.edu.cn.
Xiaoya
X
Liu
D000327:Adsorption; D000418:Albumins / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D002417:Cattle; D002645:Chickens; D007211:Indoles / Q000737:chemistry*; D054802:Molecular Imprinting / Q000379:methods*; D009113:Muramidase / Q000737:chemistry* / Q000302:isolation & purification; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D056951:Photoelectron Spectroscopy; D011108:Polymers / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman
D000418:Albumins; D007211:Indoles; D037742:Nanotubes, Carbon; D011108:Polymers; C568283:polydopamine; D009113:Muramidase
MWNTs; Molecular imprinting; Polydopamine; Protein