In vitro detection of cytotoxicity using FluoroJade-C.
We describe here a novel method for the determination of cytotoxicity in cell cultures using Fluoro-Jade C (FJ-C). FJ-C has been previously used for the assessment of neurodegeneration in fixed brain tissue samples, and has never been utilized in live cell cultures or in different types of cells other than neurons. In the present study we examined the utility of FJ-C for the determination of cytotoxicity in vitro. Various cell cultures were evaluated including neural stem cells, brain microvessel endothelial cells, and SH-SY5Y, PC12 and MDCK cells. Cytotoxicities induced by toxicants in cell cultures, as determined by the FJ-C labeling, were further confirmed by commonly used cytotoxicity assays. This in vitro approach is simple, fast, and sensitive and, thus, has the potential to augment if not replace currently used cell-based cytotoxicity assays.
Division of Neurotoxicology, National Center for Toxicological Research, U.S. Food and Drug Administration, United States. Electronic address: qiang.gu@fda.hhs.gov.
Qiang
Q
Gu
Division of Neurotoxicology, National Center for Toxicological Research, U.S. Food and Drug Administration, United States.
Susan
S
Lantz-McPeak
Division of Neurotoxicology, National Center for Toxicological Research, U.S. Food and Drug Administration, United States.
Hector
H
Rosas-Hernandez
Division of Neurotoxicology, National Center for Toxicological Research, U.S. Food and Drug Administration, United States.
Elvis
E
Cuevas
Division of Neurotoxicology, National Center for Toxicological Research, U.S. Food and Drug Administration, United States.
Syed F
SF
Ali
Division of Neurotoxicology, National Center for Toxicological Research, U.S. Food and Drug Administration, United States.
Merle G
MG
Paule
Division of Neurotoxicology, National Center for Toxicological Research, U.S. Food and Drug Administration, United States.
Sumit
S
Sarkar
D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects*; D005452:Fluoresceins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007668:Kidney / Q000166:cytology*; D051379:Mice; D058953:Neural Stem Cells / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects*; D051381:Rats
D005452:Fluoresceins; D005456:Fluorescent Dyes; C534582:fluoro-jade C; D002104:Cadmium
Cell culture; Fluorescence microscopy; Toxicity