A miR-155-dependent microRNA hierarchy in dendritic cell maturation and macrophage activation.
MicroRNAs (miRNAs) are small RNAs that play important regulatory roles in many cellular pathways. MiRNAs associate with members of the Argonaute protein family and bind to partially complementary sequences on mRNAs and induce translational repression or mRNA decay. Using deep sequencing and Northern blotting, we characterized miRNA expression in wild type and miR-155-deficient dendritic cells (DCs) and macrophages. Analysis of different stimuli (LPS, LDL, eLDL, oxLDL) reveals a direct influence of miR-155 on the expression levels of other miRNAs. For example, miR-455 is negatively regulated in miR-155-deficient cells possibly due to inhibition of the transcription factor C/EBPbeta by miR-155. Based on our comprehensive data sets, we propose a model of hierarchical miRNA expression dominated by miR-155 in DCs and macrophages.
Biochemistry Center Regensburg (BZR), Laboratory for RNA Biology, University of Regensburg, Universitätsstrasse 31, 93053 Regensburg, Germany.
Anne
A
Dueck
IST Austria (Institute of Science and Technology Austria), Am Campus 1, 3400 Klosterneuburg, Austria.
Alexander
A
Eichner
IST Austria (Institute of Science and Technology Austria), Am Campus 1, 3400 Klosterneuburg, Austria.
Michael
M
Sixt
Biochemistry Center Regensburg (BZR), Laboratory for RNA Biology, University of Regensburg, Universitätsstrasse 31, 93053 Regensburg, Germany. Electronic address: gunter.meister@vkl.uni-regensburg.de.
Gunter
G
Meister
D000818:Animals; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000378:metabolism; D002454:Cell Differentiation; D003713:Dendritic Cells / Q000166:cytology*; D005786:Gene Expression Regulation; D008262:Macrophage Activation; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*
D022782:CCAAT-Enhancer-Binding Protein-beta; D035683:MicroRNAs; C532623:Mirn155 microRNA, mouse
Dendritic cell; Macrophage; MiR-155; MiR-455; MicroRNA; Regulation of gene expression