Long-term leukocyte filtration should be avoided during extracorporeal circulation.
Filtration during extracorporeal circulation (ECC) not only removes but also activates leukocytes; therefore, long-term leukocyte filtration may cause adverse effects. In the present study, we tested this hypothesis by priming ECC with 300 mL of canine blood and examining filtration effects in 3 groups (n = 6 each) during 60 min ECC. In the control group (Group C) blood was filtrated with an arterial filter for 60 min; in long-term (Group L) and short-term (Group S) groups, blood was filtrated with a leukocyte filter for 60 and 5 min. We found that about 90% of leukocytes were removed after 5 min of filtration in both Groups L and S. Although leukocyte count continued to reduce, mean fluorescent intensities of CD11/CD18, free hemoglobin, and neutrophil elastase increased in Group L and were higher than those in Groups C and S at 60 min. Leukocyte rupture, cytoplasmic leakage, and circulating naked nuclei were also found in Group L. The data support our hypothesis that long-term filtration can induce inflammation and lead to leukocyte destruction.
Department of Anesthesiology and Translational Neuroscience Center, West China Hospital, Sichuan University, 37 Wainan Guoxuexiang, Chengdu, Sichuan 610041, China.
Jiali
J
Tang
Department of Thoracic Cardiovascular Surgery, Affiliated No. 2 Hospital, Zhejiang University, No. 88 Jiefang Road, Hangzhou, Zhejiang 310009, China.
Kaiyu
K
Tao
Department of Laboratory Medicine, West China Hospital, Sichuan University, 37 Wainan Guoxuexiang, Chengdu, Sichuan 610041, China.
Jing
J
Zhou
Department of Laboratory Medicine, West China Hospital, Sichuan University, 37 Wainan Guoxuexiang, Chengdu, Sichuan 610041, China.
Chongwei
C
Zhang
Department of Anesthesiology and Translational Neuroscience Center, West China Hospital, Sichuan University, 37 Wainan Guoxuexiang, Chengdu, Sichuan 610041, China.
Lina
L
Gong
Department of Anesthesiology and Translational Neuroscience Center, West China Hospital, Sichuan University, 37 Wainan Guoxuexiang, Chengdu, Sichuan 610041, China.
Nanfu
N
Luo
Department of Anesthesiology and Translational Neuroscience Center, West China Hospital, Sichuan University, 37 Wainan Guoxuexiang, Chengdu, Sichuan 610041, China.
Lei
L
Du
D000818:Animals; D018845:CD11 Antigens / Q000032:analysis; D018821:CD18 Antigens / Q000032:analysis; D004285:Dogs; D005112:Extracorporeal Circulation; D005374:Filtration; D006454:Hemoglobins / Q000032:analysis; D007249:Inflammation / Q000209:etiology; D047589:Leukocyte Reduction Procedures; D007962:Leukocytes / Q000502:physiology
D018845:CD11 Antigens; D018821:CD18 Antigens; D006454:Hemoglobins