Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes.
Several unique Sus-like polysaccharide utilization loci (PULs) were identified from bacteria resident in bovine rumen microbiomes through functional screening of a fosmid library. The loci were phylogenetically assigned to the genus Prevotella within the phylum Bacteroidetes. These findings were augmented by a bioinformatic re-evaluation of ruminal Prevotella genomes, revealing additional loci not previously reported in the literature. Analysis of Bacteroidales-affiliated genomes reconstructed from a bovine rumen metagenome in a previous study further expanded the diversity of Sus-like PULs resident in this microbiome. Our findings suggest that Sus-like systems represent an important mechanism for degradation of a range of plant-derived glycans in ruminants.
CSIRO Animal, Food and Health Sciences, Riverside Life Sciences Centre, PO Box 52, North Ryde, NSW, 2113, Australia, carly.rosewarne@csiro.au.
Carly P
CP
Rosewarne
Phillip B
PB
Pope
Jane L
JL
Cheung
Mark
M
Morrison
D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002417:Cattle; D004269:DNA, Bacterial / Q000235:genetics; D064307:Microbiota; D008969:Molecular Sequence Data; D010802:Phylogeny; D011134:Polysaccharides / Q000378:metabolism; D018720:Prevotella / Q000235:genetics / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012417:Rumen / Q000382:microbiology*; D013552:Swine
D004269:DNA, Bacterial; D011134:Polysaccharides; D012336:RNA, Ribosomal, 16S