Lineage tracing quantification reveals symmetric stem cell division in Drosophila male germline stem cells.
In the homeostatic state, adult stem cells divide either symmetrically to increase the stem cell number to compensate stem cell loss, or asymmetrically to maintain the population while producing differentiated cells. We have investigated the mode of stem cell division in the testes ofDrosophila melanogasterby lineage tracing and confirm the presence of symmetric stem cell division in this system. We found that the rate of symmetric division is limited to 1-2% of total germline stem cell (GSC) divisions, but it increases with expression of a cell adhesion molecule, E-cadherin, or a regulator of the actin cytoskeleton, Moesin, which may modulate adhesiveness of germ cells to the stem cell niche. Our results indicate that the decision regarding asymmetric vs. symmetric division is a dynamically regulated process that contributes to tissue homeostasis, responding to the needs of the tissue.
Life Sciences Institute, Department of Cell and Developmental Biology, University of Michigan Ann Arbor.
Viktoria
V
Salzmann
Life Sciences Institute, Department of Cell and Developmental Biology, University of Michigan Ann Arbor.
Mayu
M
Inaba
Department of Bioengineering, University of Illinois at Chicago, 851 S Morgan Street, Chicago IL 60607.
Jun
J
Cheng
Life Sciences Institute, Department of Cell and Developmental Biology, University of Michigan Ann Arbor.
Yukiko M
YM
Yamashita