Application of nucleic acid-lipid conjugates for the programmable organisation of liposomal modules.
We present a critical review of recent work related to the assembly of multicompartment liposome clusters using nucleic acids as a specific recognition unit to link liposomal modules. The asymmetry in nucleic acid binding to its non-self complementary strand allows the controlled association of different compartmental modules into composite systems. These biomimetic multicompartment architectures could have future applications in chemical process control, drug delivery and synthetic biology. We assess the different methods of anchoring DNA to lipid membrane surfaces and discuss how lipid and DNA properties can be tuned to control the morphology and properties of liposome superstructures. We consider different methods for chemical communication between the contents of liposomal compartments within these clusters and assess the progress towards making this chemical mixing efficient, switchable and chemically specific. Finally, given the current state of the art, we assess the outlook for future developments towards functional modular networks of liposomes.
School of Chemistry, University of Leeds, Leeds LS2 9JT, UK. Electronic address: p.a.beales@leeds.ac.uk.
Paul A
PA
Beales
Department of Chemical and Environmental Engineering, Yale University, New Haven, CT 06510, USA. Electronic address: kyle.vanderlick@yale.edu.
T Kyle
TK
Vanderlick
D000818:Animals; D032701:Biomimetics / Q000379:methods* / Q000639:trends; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D008954:Models, Biological; D010743:Phospholipids / Q000737:chemistry* / Q000378:metabolism
D008081:Liposomes; D010743:Phospholipids; D004247:DNA
Bionanotechnology; Compartmentalization; DNA amphiphiles; Directed assembly; Lipid vesicles