Preparation and evaluation of poly(2-hydroxyethyl aspartamide)-hexadecylamine-iron oxide for MR imaging of lymph nodes.
The purpose of this study was to synthesize biocompatible poly(2-hydroxyethyl aspartamide)-C16-iron oxide (PHEA-C16-iron oxide) nanoparticles and to evaluate their efficacy as a contrast agent for magnetic resonance imaging of lymph nodes. The PHEA-C16-iron oxide nanoparticles were synthesized by coprecipitation method. The core size of the PHEA-C16-iron oxide nanoparticles was about 5 to 7 nm, and the overall size of the nanoparticles was around 20, 60, and 150 nm in aqueous solution. The size of the nanoparticles was controlled by the amount of C16. The 3.0-T MRI signal intensity of a rabbit lymph node was effectively reduced after intravenous administration of PHEA-C16-iron oxide with the size of 20 nm. The in vitro and in vivo toxicity tests revealed the high biocompatibility of PHEA-C16-iron oxide nanoparticles. Therefore, PHEA-C16-iron oxide nanoparticles with 20-nm size can be potentially useful as T2-weighted MR imaging contrast agents for the detection of lymph nodes.
Dae Chul
DC
Ha
Ha Young
HY
Lee
Yeo Won
YW
Son
Soon Hong
SH
Yuk
Youn Woong
YW
Choi
Byung Kook
BK
Kwak
Byung Cheol
BC
Shin
College of Pharmacy, Chungnam National University, 99, Daehak-ro, Yuseong-gu, Daejeon 305-764, Republic of Korea. chocw@cnu.ac.kr.
Cheong-Weon
CW
Cho
Sun Hang
SH
Cho