Relation between the expression of mitotic centromere-associated kinesin and the progression of squamous cell carcinoma of the tongue.
OBJECTIVE
Mitotic centromere-associated kinesin (MCAK) is a microtubule depolymerase indispensable for microtubule binding during spindle formation. The purpose of this study was to investigate the association of MCAK expression with squamous cell carcinoma of the oral tongue (SCCOT).


METHODS
Immunohistochemistry was used in 47 cases of SCCOT. Determination of proliferation and migratory capabilities was performed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and Transwell chamber assay, respectively, on cells from the human tongue squamous cell carcinoma cell line Tca8113 that were transfected with MCAK small interfering RNA (siRNA).


RESULTS
MCAK expression level in oral tongue cancer tissue is significantly higher (P < .01) than that of corresponding normal tissue. In addition, high expression of MCAK is significantly associated with lymph node metastasis (P < .05) and tumor staging (P < .01). Moreover, gene silencing of MCAK suppresses proliferation and migration of Tca8113 cells (P < .05; P < .01).


CONCLUSIONS
The expression of MCAK may be associated with the progression of SCCOT.
Lecturer, Department of Pathology, Medical College of Qingdao University, Qingdao, Shandong, China.
Cheng-Qin
CQ
Wang
Lecturer, Department of Pathology, Medical College of Qingdao University, Qingdao, Shandong, China. Electronic address: xiangfenggang@163.com.
Feng-Gang
FG
Xiang
Professor and Attending Physician, Department of Pathology, Affiliated Hospital of the Medical College of Qingdao University, Qingdao, Shandong, China. Electronic address: wcq0879@126.com.
Yu-Jun
YJ
Li
Professor and Attending Physician, Department of Pathology, Affiliated Hospital of the Medical College of Qingdao University, Qingdao, Shandong, China.
Xiao-Ming
XM
Xing
Lecturer, Department of Pathology, Medical College of Qingdao University, Qingdao, Shandong, China.
Ning
N
Wang
Lecturer, Department of Pathology, Medical College of Qingdao University, Qingdao, Shandong, China.
Jing-Hua
JH
Chi
Professor and Attending Physician, Department of Pathology, Affiliated Hospital of the Medical College of Qingdao University, Qingdao, Shandong, China.
Wen-Juan
WJ
Yu
D000368:Aged; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D018450:Disease Progression; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016547:Kinesins / Q000378:metabolism*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009363:Neoplasm Proteins / Q000378:metabolism*; D009367:Neoplasm Staging; D034622:RNA Interference; D060888:Real-Time Polymerase Chain Reaction; D014062:Tongue Neoplasms / Q000378:metabolism* / Q000473:pathology
D009363:Neoplasm Proteins; D016547:Kinesins