In vitro culture thawed human ovarian tissue: NIV versus slow freezing method.
The aim of this study was to determine if the needle immersed vitrification method (NIV) can improve the growth potential of thawed ovarian tissue in vitro culture. Human ovarian cortical tissues were cryopreserved using NIV and slow freezing method. After 14 days of culture, the preservation outcomes of NIV and slow freezing groups were analyzed histologically using light microscope and apoptosis was assessed by TUNEL assay. The result showed that the percentage of morphologically abnormal primordial follicles was lower in NIV group than in slow freezing group (P < 0.05). The incidence of TUNEL-positive primordial follicles was lower in NIV group than in slow freezing group (P < 0.05). The study showed that cryopreservation of human ovarian tissue with NIV was effective in improving the growth potential of frozen-thawed ovarian tissue in vitro culture.
Reproductive Medical Center of West China 2nd University Hospital, Sichuan University, Chengdu 610041, China.
Zhun
Z
Xiao
Reproductive Medical Center of West China 2nd University Hospital, Sichuan University, Chengdu 610041, China.
Yan
Y
Wang
Department of Obstetrics and Gynecology of Sichuan Provincial Hospital for Women and Children, Chengdu 610031, China.
Ling-Ling
LL
Li
Reproductive Medical Center of West China 2nd University Hospital, Sichuan University, Chengdu 610041, China.
Shang-wei
SW
Li
D000328:Adult; D017209:Apoptosis; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D005615:Freezing; D006801:Humans; D020287:In Situ Nick-End Labeling; D006080:Ovarian Follicle / Q000166:cytology* / Q000648:ultrastructure*; D046509:Tissue Culture Techniques; D058989:Vitrification; D055815:Young Adult