[Construction and eukaryotic expression of PVAX1-hPV58mE6E7fcGB composite gene vaccine].
To construct and express a composite gene vaccine for human papillomavirus 58(HPV58)-associated cervical cancer, we inserted HPV58mE6E7 fusion gene into pCI-Fc-GPI eukaryotic expression vector, constructing a recombinant plasmid named pCI-sig-HPV58mE6E7-Fc-GPI. Then we further inserted fragment of sig-HPV58mE6E7Fc-GPI into the novel vaccine vector PVAX1-IRES-GM/B7, constructing PVAX1-HPV58mE6E7FcGB composite gene vaccine. PVAX1-HPV58mE6E7FcGB vaccine was successfully constructed and identified by restriction endonuclease and sequencing analysis. Eukaryotic expression of fusion antigen sig-HPV58mE6E7-Fc-GPI and molecular ad-juvant GM-CSF and B7. 1 were proved to be realized at the same time by flow cytometry and immunofluorescence. So PVAX1-HPV58mE6E7FcGB can be taken as a candidate of therapeutic vaccine for HPV58-associated tumors and their precancerous transformations.
Tumor Hospital of Guangxi Medical University, Nanning 530021, China.
He
H
Wang
Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
Jiyun
J
Yu
Tumor Hospital of Guangxi Medical University, Nanning 530021, China.
Li
L
Li
D019496:Cancer Vaccines / Q000096:biosynthesis* / Q000235:genetics; D036022:Capsid Proteins / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis* / Q000235:genetics; D050725:Papillomavirus E7 Proteins / Q000096:biosynthesis* / Q000235:genetics; D053918:Papillomavirus Vaccines / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D002583:Uterine Cervical Neoplasms / Q000517:prevention & control; D019444:Vaccines, DNA / Q000096:biosynthesis*
D019496:Cancer Vaccines; D036022:Capsid Proteins; C548991:E6 protein, human papillomavirus type 58; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D053918:Papillomavirus Vaccines; D011994:Recombinant Proteins; D019444:Vaccines, DNA