Development and application of an immunoaffinity column enzyme immunoassay for mycotoxin zearalenone in complicated samples.
The zearalenone (ZEA) monoclonal antibody (mAb) 2D3, one of the highest sensitivity antibodies, was developed. Based on this mAb, it was established of an immunoaffinity column (IAC) coupled with an indirect competitive enzyme-linked immunosorbent assay (icELISA). After optimization, the icELISA allowed an IC50 against ZEA of 0.02 µg L(-1). The mAb 2D3 exhibited a high recognition of ZEA (100%) and β-zearalenol (β-ZOL, 88.2%). Its cross-reactivity with α-zearalenol (α-ZOL) and β-zearalanol (β-ZAL) were found to be 4.4% and 4.6%, respectively. The IAC-icELISA method was employed to analyze ZEA contamination in food samples, compared with high-performance liquid chromatography (HPLC). The spiked assay for ZEA demonstrated the considerable recoveries for IAC-icELISA (83-93%) and HPLC (94-108%) methods. Results showed that the mAb 2D3 and IAC-icELISA method posed potential applications in sensitively determination of ZEA in maize.
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, P. R. China ; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, P. R. China ; Key laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan, P. R. China ; Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan, P. R. China.
Xiaoqian
X
Tang
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, P. R. China ; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, P. R. China ; Key laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan, P. R. China ; Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan, P. R. China.
Xin
X
Li
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, P. R. China ; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, P. R. China ; Key laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan, P. R. China ; Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan, P. R. China ; Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan, P. R. China.
Peiwu
P
Li
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, P. R. China ; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, P. R. China ; Key laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan, P. R. China.
Qi
Q
Zhang
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, P. R. China ; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, P. R. China ; Key laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan, P. R. China.
Ran
R
Li
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, P. R. China ; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, P. R. China ; Key laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan, P. R. China.
Wen
W
Zhang
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, P. R. China ; Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan, P. R. China.
Xiaoxia
X
Ding
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, P. R. China ; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, P. R. China ; Key laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan, P. R. China ; Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan, P. R. China.
Jiawen
J
Lei
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, P. R. China ; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, P. R. China ; Key laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan, P. R. China ; Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan, P. R. China.
Zhaowei
Z
Zhang
D000818:Animals; D000906:Antibodies / Q000378:metabolism; D001947:Breeding; D002846:Chromatography, Affinity / Q000379:methods*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006825:Hybridomas; D007124:Immunoenzyme Techniques / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D009183:Mycotoxins / Q000032:analysis*; D003313:Zea mays / Q000737:chemistry; D015029:Zeranol / Q000031:analogs & derivatives* / Q000032:analysis
D000906:Antibodies; D009183:Mycotoxins; C029659:zearalenol; D015029:Zeranol