Controlling ferrofluid permeability across the blood–brain barrier model.
In the present study, an in vitro blood–brain barrier model was developed using murine brain endothelioma cells (b.End3 cells). Confirmation of the blood–brain barrier model was completed by examining the permeability of FITCDextran at increasing exposure times up to 96 h in serum-free medium and comparing such values with values from the literature. After such confirmation, the permeability of five novel ferrofluid (FF) nanoparticle samples, GGB (ferrofluids synthesized using glycine, glutamic acid and BSA), GGC (glycine, glutamic acid and collagen), GGP (glycine, glutamic acid and PVA), BPC (BSA, PEG and collagen) and CPB (collagen, PVA and BSA), was determined using this blood–brain barrier model. All of the five FF samples were characterized by zeta potential to determine their charge as well as TEM and dynamic light scattering for determining their hydrodynamic diameter. Results showed that FF coated with collagen passed more easily through the blood–brain barrier than FF coated with glycine and glutamic acid based on an increase of 4.5% in permeability. Through such experiments, diverse magnetic nanomaterials (such as FF) were identified for: (1) MRI use since they were less permeable to penetrate the blood–brain barrier to avoid neural tissue toxicity (e.g. GGB) or (2) brain drug delivery since they were more permeable to the blood–brain barrier (e.g. CPB).
Di
D
Shi
Linlin
L
Sun
Gujie
G
Mi
Lubna
L
Sheikh
Soumya
S
Bhattacharya
Suprabha
S
Nayar
Thomas J
TJ
Webster
D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects*; D045744:Cell Line, Tumor; D003094:Collagen / Q000737:chemistry; D016895:Culture Media, Serum-Free / Q000737:chemistry; D003911:Dextrans / Q000737:chemistry; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D018698:Glutamic Acid / Q000737:chemistry; D005998:Glycine / Q000737:chemistry; D006801:Humans; D007501:Iron / Q000737:chemistry; D008027:Light; D008280:Magnetics; D051379:Mice; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry; D036103:Nanotechnology; D010539:Permeability; D011142:Polyvinyl Alcohol / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013997:Time Factors
D016895:Culture Media, Serum-Free; D003911:Dextrans; D012710:Serum Albumin, Bovine; D018698:Glutamic Acid; D011142:Polyvinyl Alcohol; D003094:Collagen; D007501:Iron; D016650:Fluorescein-5-isothiocyanate; D005998:Glycine