Universal marker and detection tool for human sarcoma circulating tumor cells.
To date, no specific marker exists for the detection of circulating tumor cells (CTC) from different types of sarcomas, though tools are available for detection of CTCs in peripheral blood of patients with cancer for epithelial cancers. Here, we report cell-surface vimentin (CSV) as an exclusive marker on sarcoma CTC regardless of the tissue origin of the sarcoma as detected by a novel monoclonal antibody. Utilizing CSV as a probe, we isolated and enumerated sarcoma CTCs with high sensitivity and specificity from the blood of patients bearing different types of sarcoma, validating their phenotype by single cell genomic amplification, mutation detection, and FISH. Our results establish the first universal and specific CTC marker described for enumerating CTCs from different types of sarcoma, thereby providing a key prognosis tool to monitor cancer metastasis and relapse.
Authors' Affiliations: Departments of Pediatrics and Surgical Oncology; Department of Sarcoma Medical Oncology, The University of Texas MD Anderson Cancer Center; and UTMD Graduate School of Biomedical Sciences, Houston, Texas.
Arun
A
Satelli
Abhisek
A
Mitra
Jeffry J
JJ
Cutrera
Marcos
M
Devarie
Xueqing
X
Xia
Davis R
DR
Ingram
Denada
D
Dibra
Neeta
N
Somaiah
Keila E
KE
Torres
Vinod
V
Ravi
Joseph A
JA
Ludwig
Eugenie S
ES
Kleinerman
Shulin
S
Li
D000911:Antibodies, Monoclonal / Q000737:chemistry; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000378:metabolism*; D045744:Cell Line, Tumor; D004252:DNA Mutational Analysis; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D005784:Gene Amplification; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism; D006801:Humans; D008856:Microscopy, Fluorescence; D009360:Neoplastic Cells, Circulating / Q000378:metabolism*; D011379:Prognosis; D012509:Sarcoma / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D012680:Sensitivity and Specificity; D059010:Single-Cell Analysis; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D014746:Vimentin / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D014746:Vimentin