Nodal, Nanog, DAZL and SMAD gene expression in human amniotic fluid stem cells.
The aim of this study was to investigate the expression of Nodal (NODAL Homolog (mouse), Nanog (Nanog Homeobox), DAZL (Deleted in Azoospermia Like) and SMAD (SMAD Family member) genes and their potential role in the regulation of self-renewal in human amniotic fluid-derived stem cells. In this experimental study human amniotic fluid-derived stem cells were analyzed for messenger RNA expression of Nodal. Immunocytochemistry also was performed to determine Nanog and DAZL. SMAD genes expression analysis was performed using cDNA Microarray analysis. Nodal mRNA was positively expressed in all samples of amniotic fluid derived stem cells. Amniotic fluid-derived stem cells showed strong immunoreactivity for molecular markers of undifferentiated human embryonic stem cells including Nanog and DAZL. Among the 8 SMAD genes expressions analyzed SMAD1, SMAD2, SMAD3, SMAD4 and SMAD7 showed positive expression. In conclusion amniotic fluid-derived stem cells seem to express Nodal, Nanog and DAZL and it speculated that the regulation of self-renewal in AFSc could be similar as in human embryonic stem cells.
Laboratory of Stem Cells, Division of Reproductive Medicine, Alexandra Maternity Hospital,1st Department of Obstetrics & Gynecology, University of Athens - Medical School, Greece. kstefanidis@med.uoa.gr
Konstantinos
K
Stefanidis
Laboratory of Stem Cells, Division of Reproductive Medicine, Alexandra Maternity Hospital,1st Department of Obstetrics & Gynecology, University of Athens - Medical School, Greece.
Vasileios
V
Pergialiotis
Laboratory of Stem Cells, Division of Reproductive Medicine, Alexandra Maternity Hospital,1st Department of Obstetrics & Gynecology, University of Athens - Medical School, Greece.
Dimitrios
D
Christakis
Laboratory of Stem Cells, Division of Reproductive Medicine, Alexandra Maternity Hospital,1st Department of Obstetrics & Gynecology, University of Athens - Medical School, Greece.
Dimitrios
D
Loutradis
Laboratory of Stem Cells, Division of Reproductive Medicine, Alexandra Maternity Hospital,1st Department of Obstetrics & Gynecology, University of Athens - Medical School, Greece.
Aristides
A
Antsaklis
D000328:Adult; D000653:Amniotic Fluid / Q000166:cytology* / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D000071317:Nanog Homeobox Protein; D055457:Nodal Protein / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D011247:Pregnancy; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D051785:Smad Proteins / Q000235:genetics* / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism*
C101358:DAZL protein, human; D018398:Homeodomain Proteins; C486780:NANOG protein, human; C526007:NODAL protein, human; D000071317:Nanog Homeobox Protein; D055457:Nodal Protein; D016601:RNA-Binding Proteins; D051785:Smad Proteins