[Determination of the antioxidant properties of activators of mitochondrial ATP-dependent potassium channels with the Amplex Red fluorescent indicator].
The effect of adaptogens-antihypoxants that participate in the activation of mitochondrial ATP-dependent potassium channels (mitoK(ATP)) at the oxidation of the Amplex Red (AR) fluorescent indicator in a peroxidase system was tested. It was shown that Extralife, Hypoxen, taurine, and synthetic antioxidant ionol can be arranged in the following row, according to the fluorescence inhibition activity: Extralife > Hypoxen > > ionol > taurine; their effect was shown to be concentration-dependent. The calculated K(i) value of fluorescence indicators demonstrate fast and slow phases of inhibition of the AR oxidation by Extralife and Hypoxen. The fast phase occurs in the presence of microdoses (0.05-3 microg/mL) of adaptogens and is related to the competition for H2O2, which is in agreement with our previous data on the mitoK(ATP) activation by doses of adaptogens related to the H2O2 consumption. The slow phase is characteristic of high adaptogen and ionol concentrations and is related to the competition for phenoxyl radicals of resorufin formed during AR oxidation. The obtained results allow one to suggest the application of a highly sensitive model peroxidase system with AR for the preliminary testing of compounds activating mitoK(ATP) channels.
S V
SV
Murzaeva
S P
SP
Belova
G D
GD
Mironova
D000975:Antioxidants / Q000737:chemistry*; D002084:Butylated Hydroxytoluene / Q000737:chemistry*; D005456:Fluorescent Dyes; D006801:Humans; D008928:Mitochondria / Q000737:chemistry / Q000378:metabolism; D010078:Oxazines / Q000032:analysis* / Q000737:chemistry; D010647:Phenyl Ethers / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D015221:Potassium Channels / Q000819:agonists / Q000737:chemistry; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013654:Taurine / Q000737:chemistry*
D000975:Antioxidants; D005456:Fluorescent Dyes; D010078:Oxazines; D010647:Phenyl Ethers; D010936:Plant Extracts; D015221:Potassium Channels; D012996:Solutions; C527119:extralife; C117780:mitochondrial K(ATP) channel; C470430:Amplex Red; C085806:olifen; D013654:Taurine; D002084:Butylated Hydroxytoluene