DRM1 and DRM2 expression regulation: potential role of splice variants in response to stress and environmental factors in Arabidopsis.
The DRM1/ARP gene family is increasingly exhibiting associations with stress conditions at the transcript level. Traditionally correlated with dormancy, increases in transcript levels in response to various treatments have also been reported in various species. As alternative transcript splicing is common in stress conditions, the splice variants of AtDRM1 and AtDRM2 were assessed further in this study. A previously undescribed splice variant of AtDRM1 (AtDRM1.6) is introduced in this work. In silico analyses of predicted protein sequence of all splice variants showed that all variants retain the predicted intrinsically disordered nature. Transcriptional studies of AtDRM1 and AtDRM2 in response to a wide range of abiotic, physical and hormonal treatments showed that AtDRM1.6 is differentially regulated at the transcriptional level compared with other splice variants. Promoter analyses demonstrated AtDRM1 light regulation via the upstream promoter sequence.
The New Zealand Institute for Plant and Food Research Limited, Mt Albert, Private Bag 92169, Auckland Mail Centre, Auckland, 1142, New Zealand, georgina.rae@plantandfood.co.nz.
Georgina M
GM
Rae
Vladimir N
VN
Uversky
Karine
K
David
Marion
M
Wood
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D059647:Gene-Environment Interaction; D064267:Intrinsically Disordered Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008027:Light; D008780:Methyltransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010641:Phenotype; D011401:Promoter Regions, Genetic; D055049:Salt Tolerance / Q000235:genetics; D016415:Sequence Alignment; D013312:Stress, Physiological; D014158:Transcription, Genetic
D064267:Intrinsically Disordered Proteins; D008780:Methyltransferases; C470731:DRM1 protein, Arabidopsis; C470732:DRM2 protein, Arabidopsis