Comparative analyses of histone H3K9 trimethylations in the heart and spleen of normal humans.
The global features of trimethylations of histone 3 at lysine 9 (H3K9me3) have been well studied in recent years; however, most of these studies were performed in mammalian cell lines. In this study, we generated genome-wide maps of H3K9me3 of the human heart and spleen using chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) technology. We examined the global patterns of H3K9me3 in both tissues and found that modifications were closely associated with tissue-specific expression, function, and development. In addition, we found that 169 genes displayed significant H3K9me3 differences between the heart and spleen. Among these genes, 64 were heart-H3K9me3-specific, 87 genes were spleen-H3K9me3-specific, and 18 were shared in both heart- and spleen-H3K9me3. In conclusion, we observed significant differences in H3K9me3 in the heart and spleen, which may help to explain epigenetic differences between these tissues. Such novel findings highlight the significance of H3K9me3 as a potential biomarker or promising target for epigenetic-based disease treatment.
Nephrology Department of Guilin 181st Hospital, Guangxi Key Laboratory of Metabolic Diseases Research, Guilin, Guangxi, China.
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Sui
Nephrology Department of Guilin 181st Hospital, Guangxi Key Laboratory of Metabolic Diseases Research, Guilin, Guangxi, China.
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Cao
Nephrology Department of Guilin 181st Hospital, Guangxi Key Laboratory of Metabolic Diseases Research, Guilin, Guangxi, China.
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Che
Nephrology Department of Guilin 181st Hospital, Guangxi Key Laboratory of Metabolic Diseases Research, Guilin, Guangxi, China.
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Chen
Nephrology Department of Guilin 181st Hospital, Guangxi Key Laboratory of Metabolic Diseases Research, Guilin, Guangxi, China.
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Xue
Nephrology Department of Guilin 181st Hospital, Guangxi Key Laboratory of Metabolic Diseases Research, Guilin, Guangxi, China.
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Liu
College of Life Science, Guangxi Normal University, Guilin, Guangxi, China.
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Guo
Clinical Medical Research Center, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, Guangdong, China daiyong2222@gmail.com.
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Dai
D000818:Animals; D002843:Chromatin / Q000235:genetics*; D057890:Epigenomics; D015894:Genome, Human; D056466:Histone Demethylases / Q000235:genetics; D006657:Histones / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008745:Methylation; D009206:Myocardium / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D013154:Spleen / Q000378:metabolism
D002843:Chromatin; D006657:Histones; D056466:Histone Demethylases