Performance and microbial community dynamics in a two-phase anaerobic co-digestion system using cassava dregs and pig manure.
The two-phase anaerobic co-digestion of cassava dregs (CD) with pig manure (PM) was evaluated using four sequencing batch reactors (SBRs) and a continuously stirred tank reactor (CSTR). The effect of seven different PM to CD volatile solid ratios (10:0, 8:2, 6:4, 5:5, 4:6, 2:8 and 0:10) on the acidification phase was investigated. Results indicated the concentrations of soluble chemical oxygen demand, NH4-N and volatile fatty acids increased substantially at seven ratios. Co-acidification of PM and CD performed well. Methanogenic fermentation of the acidification products at seven ratios was steady in CSTR. The highest methane yield and VS removal of 0.352m(3)/kg VSadded and 68.5% were achieved at PM:CD (4:6). The microbial population in CSTR was analyzed using molecular methods. Findings revealed that bacteria such as Firmicutes and Bacteroidetes, archaea such as Methanobacteriales and Methanomicrobiales were advantageous populations. Co-digestion of PM and CD supported higher quantity and diversity of methanogens.
College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University, Beijing 100193, China.
Jiwei
J
Ren
College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University, Beijing 100193, China.
Xufeng
X
Yuan
College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University, Beijing 100193, China.
Jie
J
Li
College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University, Beijing 100193, China.
Xuguang
X
Ma
College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University, Beijing 100193, China.
Ye
Y
Zhao
College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University, Beijing 100193, China.
Wanbing
W
Zhu
College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University, Beijing 100193, China.
Xiaofen
X
Wang
College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University, Beijing 100193, China. Electronic address: acuizj@cau.edu.cn.
Zongjun
Z
Cui
D000641:Ammonia / Q000378:metabolism; D000818:Animals; D001421:Bacteria, Anaerobic / Q000378:metabolism*; D041963:Bacteroidetes / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors; D016000:Cluster Analysis; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D005232:Fatty Acids, Volatile / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000235:genetics / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D002365:Manihot / Q000737:chemistry / Q000378:metabolism*; D008372:Manure / Q000032:analysis*; D008697:Methane / Q000096:biosynthesis; D017012:Methanobacteriales / Q000235:genetics / Q000378:metabolism; D017018:Methanomicrobiales / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013552:Swine
D017931:DNA Primers; D005232:Fatty Acids, Volatile; D008372:Manure; D012336:RNA, Ribosomal, 16S; D000641:Ammonia; D008697:Methane
Cassava dregs; Co-digestion; Methanogens; Pig manure; Two-phase