Construction and functional analysis of novel dominant-negative mutant of human SOX18 protein.
SOX18 transcription factor plays important roles in a range of biological processes such as vasculogenesis, hair follicle development, lymphangiogenesis, atherosclerosis, and angiogenesis. In this paper we present the generation of a novel SOX18 dominant-negative mutant (SOX18DN) encoding truncated SOX18 protein that lacks a trans-activation domain. We show that both wild-type SOX18 (SOX18wt) and truncated human SOX18 proteins are able to bind to their consensus sequence in vitro. Functional analysis confirmed that SOX18wt has potent trans-activation properties, while SOX18DN displays dominant-negative effect. We believe that these SOX18wt and SOX18DN expression constructs could be successfully used for further characterization of the function of this protein.
Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, 11010, Serbia. isidorapetrovic@imgge.bg.ac.rs.
M
M
Milivojevic
I
I
Petrovic
N
N
Kovacevic-Grujicic
J
J
Popovic
M
M
Mojsin
M
M
Stevanovic
D001483:Base Sequence; D017930:Genes, Reporter; D006367:HeLa Cells; D006801:Humans; D009154:Mutation; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D055760:SOXF Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D015533:Transcriptional Activation
C095473:SOX18 protein, human; D055760:SOXF Transcription Factors