Immobilization of biomolecules on cysteamine-modified polyaniline film for highly sensitive biosensing.
We present a new cysteamine (CS)-modified polyaniline (PANI) film for highly efficient immobilization of biomolecules in biosensing technology. This electrochemical deposited PANI film treated with CS and glutaraldehyde could be employed as an excellent substrate for biomolecules immobilization. The parameters of PANI growth were optimized to obtain suitable surface morphology of films for biomolecules combination with the help of electron and atomic force microscopy. Cyclic voltammetry (CV) was utilized to illustrate the different electrochemical activities of each modified electrode. Due to the existence of sulfydryl group and amino group in CS, surface modification with CS was proven to reduce oxidized units on PANI film remarkably, as evidenced by both ATR-FTIR and Raman spectroscopy characterizations. Furthermore, bovine serum albumin (BSA) was used as the model protein to investigate the immobilization efficiency of biomolecules on the PANI film, comparative study using quartz crystal microbalance (QCM) showed that BSA immobilized on CS-modified PANI could be increased by at least 20% than that without CS-modified PANI in BSA solution with the concentration of 0.1-1mg/mL. The CS-modified PANI film would be significant for the immobilization and detection of biomolecules and especially promising in the application of immunosensor for ultrasensitive detection.
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, No. 865, Changning Road, Shanghai 200050, China; University of Chinese Academy of Sciences, No. 19A, Yuquan Road, Beijing 100049, China.
Qi
Q
Cai
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, No. 865, Changning Road, Shanghai 200050, China.
Baojian
B
Xu
Sate Key Laboratory of Functional Materials for Informatics, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, No. 865, Changning Road, Shanghai 200050, China; University of Chinese Academy of Sciences, No. 19A, Yuquan Road, Beijing 100049, China.
Lin
L
Ye
Sate Key Laboratory of Functional Materials for Informatics, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, No. 865, Changning Road, Shanghai 200050, China.
Zengfeng
Z
Di
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, No. 865, Changning Road, Shanghai 200050, China.
Jishen
J
Zhang
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, No. 865, Changning Road, Shanghai 200050, China.
Qinghui
Q
Jin
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, No. 865, Changning Road, Shanghai 200050, China. Electronic address: jlzhao@mail.sim.ac.cn.
Jianlong
J
Zhao
SGS China Co., Ltd., 4F, Building 3, No. 889, Yishan Road, Shanghai 200233, China.
Jian
J
Xue
School of Electronic Engineering, Bangor University, Bangor LL57 1UT, United Kingdom. Electronic address: x.chen@bangor.ac.uk.
Xianfeng
X
Chen
D000814:Aniline Compounds / Q000737:chemistry*; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D002417:Cattle; D003543:Cysteamine / Q000737:chemistry*; D004566:Electrodes; D055767:Immobilized Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*
D000814:Aniline Compounds; D055767:Immobilized Proteins; C416807:polyaniline; D012710:Serum Albumin, Bovine; D003543:Cysteamine
Biosensing; Cysteamine; Immobilization efficiency; Polyaniline film; Quartz crystal microbalance (QCM)