Co-delivery of siRNAs and anti-cancer drugs using layered double hydroxide nanoparticles.
In this research we employed layered double hydroxide nanoparticles (LDHs) to simultaneously deliver an anticancer drug 5-fluorouracil (5-FU) and Allstars Cell Death siRNA (CD-siRNA) for effective cancer treatment. The strategy takes advantage of the LDH anion exchange capacity to intercalate 5-FU into its interlayer spacing and load siRNA on the surface of LDH nanoparticles. LDH nanoparticles have been previously demonstrated as an effective cellular delivery system for 5-FU and siRNA separately in various investigations. More excitedly, the combination of CD-siRNA and anticancer drug 5-FU with the same LDH particles significantly enhanced cytotoxicity to three cancer cell lines, e.g. MCF-7, U2OS and HCT-116, compared to the single treatment with either CD-siRNA or 5-FU. This enhancement is probably a result of coordinate mitochondrial damage process. Thus, the strategy to co-deliver siRNA and an anticancer drug by LDHs has great potential to overcome the drug resistance and enhance cancer treatment.
Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia.
Li
L
Li
Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia. Electronic address: w.gu@uq.edu.au.
Wenyi
W
Gu
School of Biomedical Sciences, University of Queensland, St Lucia, QLD 4072, Australia; Faculty of Science, Medicine and Health, University of Wollongong, Northfields Avenue, NSW 2522, Australia.
Jiezhong
J
Chen
Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia.
Weiyu
W
Chen
Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia. Electronic address: gordonxu@uq.edu.au.
Zhi P
ZP
Xu
D000970:Antineoplastic Agents / Q000008:administration & dosage*; D017209:Apoptosis; D015153:Blotting, Western; D045744:Cell Line, Tumor; D006801:Humans; D006878:Hydroxides / Q000737:chemistry*; D053758:Nanoparticles; D034741:RNA, Small Interfering / Q000008:administration & dosage*
D000970:Antineoplastic Agents; D006878:Hydroxides; D034741:RNA, Small Interfering
Apoptosis; Co-delivery system; Electrostatic assembly; Layered double hydroxide