The effect of anthocyanin on the prostate in an andropause animal model: rapid prostatic cell death by apoptosis is partially prevented by anthocyanin supplementation.
OBJECTIVE
To evaluate the anti-apoptotic effect of the antioxidant reaction of anthocyanin on the prostate in an andropause animal model.


METHODS
Sprague-Dawley rats were divided into three groups (n=12 in each): control (Group I), andropause (Group II), andropause treated with anthocyanin (Group III). For induction of andropause, Group II and III underwent bilateral orchiectomy. Group III was treated with daily oral anthocyanin (160 mg/kg) for 8 weeks. After 8 weeks, the rats were sacrificed and their blood and prostates were examined pathohistologically and evaluated for oxidative stress and apoptosis. Oxidative stress was assessed by the activity of superoxide dismutase (SOD) and apoptosis in the prostate was identified by terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labelling assay.


RESULTS
Group II showed markedly increased activity of SOD in serum over that observed in Group I, whereas the rats in Group III showed reduced oxidative stress compared to Group II. Despite no significant differences in prostate weight between Group II and III (p=0.078), the apoptotic index was significantly greater in Group II than Group I, and was significantly lesser in Group III than Group II.


CONCLUSIONS
We suggest that the oxidative stress caused by low testosterone may be another inducer of apoptosis, and this apoptosis may partly contribute to the overall apoptosis of the prostate in the andropause animal model. Therefore, anthocyanin supplementation may contribute to preventing excessively rapid cell death by apoptosis in the prostate in an animal model of andropause.
Department of Urology, The Catholic University of Korea College of Medicine, Seoul, Korea.
Hoon
H
Jang
Department of Urology, The Catholic University of Korea College of Medicine, Seoul, Korea.
Woong Jin
WJ
Bae
Health Promotion Center, The Catholic University of Korea, Seoul St. Mary's Hospital, Seoul, Korea.
Su Jin
SJ
Kim
Department of Urology, The Catholic University of Korea College of Medicine, Seoul, Korea.
Seoung Mo
SM
Yuk
Department of Urology, The Catholic University of Korea College of Medicine, Seoul, Korea.
Dong Seok
DS
Han
Department of Urology, The Catholic University of Korea College of Medicine, Seoul, Korea.
U Syn
US
Ha
Korea BioMedical Science Institute, Seoul, Korea.
Sung Yeoun
SY
Hwang
The Catholic Agro-Medical Center, The Catholic University of Korea, Seoul, Korea.
Shin Hee
SH
Yoon
Department of Urology, Second Hospital of Lanzhou University, Lanzhou, China.
Zhiping
Z
Wang
Department of Urology, The Catholic University of Korea College of Medicine, Seoul, Korea.
Sae Woong
SW
Kim
Andropause; Anthocyanins; Antioxidants; Apoptosis