Expression and promoter analysis of six heat stress-inducible genes in rice.
During the long evolutionary process, plant gradually formed a series of strategies and mechanisms to cope with stress environment such as drought, heat, cold, and high salinity. Six highly heat responsive genes were identified in rice by microarray data analysis. The qRT-PCR analysis confirmed that the expression of these six genes were highly heat inducible and moderately responded to salt stress, polyethylene glycol, and abscisic acid treatment, but little affected by cold treatment. Promoters of the three highly heat-inducible genes (OsHsfB2cp, PM19p, and Hsp90p) were used to drive GUS gene expression in rice. The results of the GUS gene expression, histochemical staining, and GUS activities in panicles and flag leaves of the transgenic rice plants confirmed high heat-induced GUS activities and moderate drought-induced activities. The three promoters exhibited similar high activity lever in rice leaf under heat, but OsHsfB2cp and PM19p showed much higher activities in panicles under heat stress. Our work confirmed that the OsHsfB2c and PM19 promoters were highly heat inducible and further characterization and reconstruction of cis-elements in their promoters could lead to the development of highly effective heat-inducible promoters for plant genetic engineering.
Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University, Changsha 410128, China.
Wirat
W
Rerksiri
Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University, Changsha 410128, China ; College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China.
Xianwen
X
Zhang
Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University, Changsha 410128, China ; Center of Analysis and Testing, Hunan Agricultural University, Changsha 410128, China.
Hairong
H
Xiong
Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University, Changsha 410128, China ; College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China.
Xinbo
X
Chen
D000040:Abscisic Acid; D000220:Adaptation, Biological / Q000235:genetics*; D005075:Biological Evolution; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D018869:Heat-Shock Response / Q000235:genetics*; D046228:Microarray Analysis; D012275:Oryza / Q000235:genetics*; D011092:Polyethylene Glycols; D011401:Promoter Regions, Genetic / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D054712:Salinity
D017931:DNA Primers; D011092:Polyethylene Glycols; D000040:Abscisic Acid