Phenolic profile of dark-grown and photoperiod-exposed Hypericum perforatum L. Hairy root cultures.
Hypericum perforatum L. is a medicinal plant considered as an important natural source of secondary metabolites with a wide range of pharmacological attributes. Hairy roots (HR) were induced from root segments of in vitro grown seedlings from H. perforatum after cocultivation with Agrobacterium rhizogenes A4. Investigations have been made to study the production of phenolic compounds in dark-grown (HR1) and photoperiod-exposed (HR2) cultures. The chromatographic analysis of phenolic acids, flavonols, flavan-3-ols, and xanthones revealed marked differences between HR1 and HR2 cultures. The production of quinic acid, kaempferol, and seven identified xanthones was increased in HR2. Moreover, HR2 showed a capability for de novo biosynthesis of two phenolic acids (3-p-coumaroylquinic acid and 3-feruloylquinic acid), three flavonol glycosides (kaempferol hexoside, hyperoside, and quercetin acetylglycoside), and five xanthones (tetrahydroxy-one-methoxyxanthone, 1,3,5-trihydroxy-6-methoxyxanthone, 1,3,5,6-tetrahydroxy-2-prenylxanthone, paxanthone, and banaxanthone E). On the other side, HR1 cultures were better producers of flavan-3-ols (catechin, epicatechin, and proanthocyanidin dimers) than HR2. This is the first comparative study on phenolic profile of H. perforatum HR cultures grown under dark and photoperiod conditions.
Department of Plant Physiology, Institute of Biology, Faculty of Natural Sciences and Mathematics, "Ss. Cyril and Methodius" University, P.O. Box 162, 1000 Skopje, Macedonia.
Oliver
O
Tusevski
Department of Analytical Chemistry, Institute of Chemistry, Faculty of Natural Sciences and Mathematics, "Ss. Cyril and Methodius" University, P.O. Box 162, 1000 Skopje, Macedonia.
Jasmina
J
Petreska Stanoeva
Department of Analytical Chemistry, Institute of Chemistry, Faculty of Natural Sciences and Mathematics, "Ss. Cyril and Methodius" University, P.O. Box 162, 1000 Skopje, Macedonia.
Marina
M
Stefova
Department of Plant Physiology, Institute of Biology, Faculty of Natural Sciences and Mathematics, "Ss. Cyril and Methodius" University, P.O. Box 162, 1000 Skopje, Macedonia.
Sonja Gadzovska
SG
Simic
D060054:Agrobacterium / Q000254:growth & development / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003624:Darkness; D005419:Flavonoids / Q000378:metabolism; D062385:Hydroxybenzoates / Q000378:metabolism; D020902:Hypericum / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry / Q000378:metabolism*; D017440:Photoperiod; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects; D010946:Plants, Medicinal / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects; D021241:Spectrometry, Mass, Electrospray Ionization; D046509:Tissue Culture Techniques; D044004:Xanthones / Q000378:metabolism
D005419:Flavonoids; D062385:Hydroxybenzoates; D010636:Phenols; D044004:Xanthones; C017616:phenolic acid