Puerarin blocks the signaling transmission mediated by P2X3 in SG and DRG to relieve myocardial ischemic damage.
P2X₃ receptors in stellate ganglia (SG) and cervical dorsal root ganglia (DRG) neurons are involved in sympathoexcitatory reflex induced by myocardial ischemic damage. Puerarin, a major active ingredient extracted from the traditional Chinese plant medicine Ge-gen, has been widely used in treatment of myocardial and cerebral ischemia. The present study is aimed to observe the effects of puerarin on the signaling transmission mediated by P2X₃ receptor in SG and DRG after myocardial ischemic damage. Our results showed that systolic blood pressure and heart rate increased, and the expression levels of P2X₃ mRNA and protein in SG and DRG were up-regulated after myocardial ischemic damage. Puerarin reduced systolic blood pressure and heart rate, relieved pain and decreased up-regulated expression of P2X₃ mRNA and protein in SG and DRG after myocardial ischemia. Puerarin inhibited the up-regulated ATP-activated currents in DRG neurons after myocardial ischemia. Thus, puerarin can relieve myocardial ischemic damage through blocking the P2X₃ signaling transmission and then depressed the aggravated sympathoexcitatory reflex.
Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
Shuangmei
S
Liu
Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
Chunping
C
Zhang
Orthopedics Department of Second Affiliated Hospital, Medical School of Nanchang University, Nanchang 330006, PR China.
Qingming
Q
Shi
Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
Guilin
G
Li
Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
Miaomiao
M
Song
Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
Yun
Y
Gao
Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
Changshui
C
Xu
Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
Hong
H
Xu
Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
Bo
B
Fan
Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
Shicheng
S
Yu
Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
Chaoran
C
Zheng
Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
Qicheng
Q
Zhu
Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
Bing
B
Wu
Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
Lichao
L
Peng
Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
Huangui
H
Xiong
Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
Qin
Q
Wu
Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China. Electronic address: liangsd@hotmail.com.
Shangdong
S
Liang
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D005260:Female; D005727:Ganglia, Spinal / Q000187:drug effects* / Q000503:physiopathology; D006339:Heart Rate / Q000187:drug effects / Q000502:physiology; D007529:Isoflavones / Q000494:pharmacology*; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D017202:Myocardial Ischemia / Q000188:drug therapy* / Q000503:physiopathology; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D059408:Pain Management; D018373:Peripheral Nervous System Agents / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D058477:Receptors, Purinergic P2X3 / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects* / Q000502:physiology; D013233:Stellate Ganglion / Q000187:drug effects* / Q000503:physiopathology
D007529:Isoflavones; D018373:Peripheral Nervous System Agents; D012333:RNA, Messenger; D058477:Receptors, Purinergic P2X3; D000255:Adenosine Triphosphate; C033607:puerarin
Dorsal root ganglia; Myocardial ischemia; P2X(3) receptor; Puerarin; Stellate ganglia