Immunoproteomic Analysis of the Excretory-Secretory Proteins from Spirometra mansoni Sparganum.
BACKGROUND
Sparganosis is caused by the invasion of Spirometra sparganum into various tissues/organs. Subcutaneous sparganosis can be diagnosed by biopsy, while visceral/cerebral sparganosis is not easy to be diagnosed. The diagnosis depends largely on the detection of specific anti-sparganum antibodies. The specificity of the ELISA could be increased by using S. mansoni sparganum excretory-secretory (ES) antigens, but it also had the cross-reactions with sera of patients with cysticercosis or paragonimiasis. The aim of this study was to identify early specific diagnostic antigens in S. mansoni sparganum ES proteins.


METHODS
The sparganum ES proteins were analyzed by two-dimensional electrophoresis (2-DE) and Western blot probed with early sera from infected mice at 14 days post-infection. The immunoreactive protein spots were characterized by MALDI-TOF/ TOF-MS.


RESULTS
A total of approximately 149 proteins spots were detected with isoelectric point (pI) varying from 3 to 7.5 and molecular weight from 20 to 115 kDa and seven protein spots with molecular weight of 23-31 kDa were recognized by the infection sera. Three of seven spots were successfully identified and characterized as the same S. mansoni protein (cysteine protease), and the proteins of other 4 spots were not included in the databases.


CONCLUSIONS
The cysteine protease from S. mansoni ES proteins recognized by early infection sera might be the early diagnostic antigens for sparganosis.
Dept. of Parasitology, Medical College, Zhengzhou University, Zhengzhou, P. R. China.
Dan Dan
DD
Hu
Dept. of Parasitology, Medical College, Zhengzhou University, Zhengzhou, P. R. China.
Jing
J
Cui
Dept. of Parasitology, Medical College, Zhengzhou University, Zhengzhou, P. R. China.
Li
L
Wang
Dept. of Parasitology, Medical College, Zhengzhou University, Zhengzhou, P. R. China.
Li Na
LN
Liu
Dept. of Parasitology, Medical College, Zhengzhou University, Zhengzhou, P. R. China.
Tong
T
Wei
Dept. of Parasitology, Medical College, Zhengzhou University, Zhengzhou, P. R. China.
Zhong Quan
ZQ
Wang
(sparganum); Diagnosis; Excretory-secretory; Plerocercoid; Spirometra mansoni