Absence of in vitro genotoxicity potential of the mycotoxin deoxynivalenol in bacteria and in human TK6 and HepaRG cell lines.
Deoxynivalenol (DON) is the major mycotoxin detected in cereal foods and a risk for human health following DON ingestion could not be excluded due to high level exposure. In this light, the hazard of DON must be carefully evaluated. Therefore, the aim of this study is to perform in vitro genotoxicity tests with DON using the Salmonella typhimurium reverse mutation assay (Ames' test), the comet assay and the micronucleus test in accordance with the OECD test guideline 487 in two human cell lines: the lymphoblastoid TK6 and the hepatoma HepaRG cells. DON gave negative results in the Ames' test performed, both with and without rat liver S9 on three strains TA98, TA100 and TA102. DON elicited cytotoxicity in TK6 and HepaRG cells but did not induce primary DNA damage. DON failed also to induce MN formation in TK6 cells with or without human and rat liver S9. After 24h of treatment, DON induced micronucleus formation in TK6 cells but only at concentrations producing more than 55 ± 5% cytotoxicity. In HepaRG cells, DON highly increased the caspase-3/7 activity but no micronucleus induction was observed. Taken together, our results suggest that DON could be considered as a non in vitro genotoxin.
ANSES, French Agency for Food, Environmental and Occupational Health & Safety, Fougères Laboratory, Toxicology of Contaminants Unit, Fougères, France. Electronic address: natsuko.mikogami@gmail.fr.
Natsuko
N
Takakura
Institut Pasteur de Lille, Laboratory of Toxicology, EA 4483 Lille, France. Electronic address: fabrice.nesslany@pasteur-lille.fr.
Fabrice
F
Nesslany
ANSES, French Agency for Food, Environmental and Occupational Health & Safety, Fougères Laboratory, Toxicology of Contaminants Unit, Fougères, France. Electronic address: valerie.fessard@anses.fr.
Valérie
V
Fessard
ANSES, French Agency for Food, Environmental and Occupational Health & Safety, Fougères Laboratory, Toxicology of Contaminants Unit, Fougères, France. Electronic address: Ludovic.lehegarat@anses.fr.
Ludovic
L
Le Hegarat
D001419:Bacteria / Q000187:drug effects* / Q000235:genetics; D004249:DNA Damage; D006801:Humans; D066298:In Vitro Techniques; D009152:Mutagenicity Tests; D009183:Mycotoxins / Q000494:pharmacology*; D017354:Point Mutation; D014255:Trichothecenes / Q000494:pharmacology*
D009183:Mycotoxins; D014255:Trichothecenes; C007262:deoxynivalenol
Comet; Deoxynivalenol; Genotoxicity; HepaRG; Micronucleus; TK6