Differential expression of cro, the lysogenic cycle repressor determinant of bacteriophage A2, in Lactobacillus casei and Escherichia coli.
Expression of bacteriophage A2-encoded cro in Escherichia coli gives rise to two co-linear polypeptides, Cro and Cro*, which were proposed to form a regulatory tandem to modulate the frequency with which the phage would choose between the lytic and the lysogenic cycles. In this communication, it is reported that Cro is the canonical product of the gene cro while Cro* results from a -1 ribosome frameshift during translation and is twelve amino acids shorter than Cro. However, frameshifting was not observed during phage development in Lactobacillus casei. Furthermore, wild type phages and cro-frameshifting negative mutants present the same phenotype, thus corroborating that only the canonical form of Cro is needed to produce a viable phage progeny.
Area de Microbiología, Instituto Universitario de Biotecnología, Facultad de Medicina, Universidad de Oviedo, Julián Clavería 6, 33006 Oviedo, Spain. Electronic address: smariconda@yahoo.com.
Susana
S
Escobedo
Area de Microbiología, Instituto Universitario de Biotecnología, Facultad de Medicina, Universidad de Oviedo, Julián Clavería 6, 33006 Oviedo, Spain. Electronic address: isabel.rodriguez@es.vwr.com.
Isabel
I
Rodríguez
Area de Microbiología, Instituto Universitario de Biotecnología, Facultad de Medicina, Universidad de Oviedo, Julián Clavería 6, 33006 Oviedo, Spain; Instituto de Productos Lácteos de Asturias (CSIC), Paseo Rio Linares, 33300 Villaviciosa, Spain. Electronic address: pgarcia@ipla.csic.es.
Pilar
P
García
Area de Microbiología, Instituto Universitario de Biotecnología, Facultad de Medicina, Universidad de Oviedo, Julián Clavería 6, 33006 Oviedo, Spain; Instituto de Productos Lácteos de Asturias (CSIC), Paseo Rio Linares, 33300 Villaviciosa, Spain. Electronic address: evaristo@uniovi.es.
Juan E
JE
Suárez
Area de Microbiología, Instituto Universitario de Biotecnología, Facultad de Medicina, Universidad de Oviedo, Julián Clavería 6, 33006 Oviedo, Spain. Electronic address: bcarrasc@cnb.csic.es.
Begoña
B
Carrasco
D001435:Bacteriophages / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D007780:Lacticaseibacillus casei / Q000821:virology*; D008242:Lysogeny; D012097:Repressor Proteins / Q000096:biosynthesis*; D014764:Viral Proteins / Q000096:biosynthesis*
D012097:Repressor Proteins; D014764:Viral Proteins
Bacteriophage A2; Cro repressor; Lactobacillus casei; Lysogenic cycle; Lytic cycle; Ribosome frameshift