Sensitive determination of reactive oxygen species in cigarette smoke using microchip electrophoresis-localized surface plasmon resonance enhanced fluorescence detection.
A sensitive approach to the determination of reactive oxygen species (ROS) in puffs of cigarette smoke (CS) has been developed. The experimental system consists of a microfluidic chip electrophoresis and a laser induced fluorescence (LIF) device enhanced by localized surface plasmon resonance. Core-shell Ag@SiO2 nanoparticles were prepared and then immobilized on the surface of the microchannel to increase the fluorescence intensity based on localized surface plasmon resonance-enhanced fluorescence (LSPREF) effect. The ROS in puffs of CS were trapped via the oxidation of 2',7'-dichlorodihydrofluorescein (DCHF) that had been loaded on polyacrylonitrile (PAN) nanofibers in a micro-column. Determination of ROS was based on the amount of 2',7'-dichlorofluorescein (DCF), which is the sole product from DCHF oxidation. With the optimization of the trapping efficiency, we detected about 8.0 pmol of ROS per puff in the mainstream CS. This microchip electrophoresis-SPREF system enables sensitive quantitation of ROS in CS with low consumption of reagent, material, and analysis time.
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, China. xhxia@nju.edu.cn.
Huai-Song
HS
Wang
Fang-Nan
FN
Xiao
Zhong-Qiu
ZQ
Li
Jun
J
Ouyang
Zeng-Qiang
ZQ
Wu
Xing-Hua
XH
Xia
Guo-Jun
GJ
Zhou
D000180:Acrylic Resins / Q000737:chemistry; D005452:Fluoresceins / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000032:analysis*; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry; D012834:Silver / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods; D014028:Tobacco Smoke Pollution / Q000032:analysis*
D000180:Acrylic Resins; D005452:Fluoresceins; D017382:Reactive Oxygen Species; D014028:Tobacco Smoke Pollution; C065013:2',7'-dichlorodihydrofluorescein; C010504:polyacrylonitrile; D012834:Silver; D012822:Silicon Dioxide