Insights into miRNA regulation of the human glycome.
Glycosylation is an intricate process requiring the coordinated action of multiple proteins, including glycosyltransferases, glycosidases, sugar nucleotide transporters and trafficking proteins. Work by several groups points to a role for microRNA (miRNA) in controlling the levels of specific glycosyltransferases involved in cancer, neural migration and osteoblast formation. Recent work in our laboratory suggests that miRNA are a principal regulator of the glycome, translating genomic information into the glycocode through tuning of enzyme levels. Herein we overlay predicted miRNA regulation of glycosylation related genes (glycogenes) onto maps of the common N-linked and O-linked glycan biosynthetic pathways to identify key regulatory nodes of the glycome. Our analysis provides insights into glycan regulation and suggests that at the regulatory level, glycogenes are non-redundant.
Biomedical Research Institute, Department of Chemistry, New York University, 100 Washington Square East, Room 1001, New York, NY 10003, United States.
Brian T
BT
Kasper
Biomedical Research Institute, Department of Chemistry, New York University, 100 Washington Square East, Room 1001, New York, NY 10003, United States.
Sujeethraj
S
Koppolu
Biomedical Research Institute, Department of Chemistry, New York University, 100 Washington Square East, Room 1001, New York, NY 10003, United States. Electronic address: lkmahal@nyu.edu.
Lara K
LK
Mahal
D053898:Biosynthetic Pathways; D005786:Gene Expression Regulation; D006031:Glycosylation; D016695:Glycosyltransferases / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D011134:Polysaccharides / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D035683:MicroRNAs; D011134:Polysaccharides; D016695:Glycosyltransferases
Carbohydrate pathways; Glycan biosynthesis; Glycan regulation; Glycogenes; Glycosylation; MicroRNA; miRNA