Localization of sirtuins in the mouse inner ear.
CONCLUSIONS
It is suggested that SIRT1 and 3, and probably SIRT4 and 5, play an important role in the neuroprotection of the inner ear. SIRT2 may be related to neuroprotection and myelin sheath formation, while SIRT6 seems to have a significant role in maintaining the energy balance by metabolic regulation.


OBJECTIVE
To analyze the expression of sirtuins (SIRT1-7) in the normal mouse inner ear.


METHODS
CBA/J mice were used for this study. The localization of SIRT1-7 in the inner ear, i.e. cochlea, vestibular end organs, and endolymphatic sac, was investigated using real-time PCR and immunohistochemistry.


RESULTS
We found high levels of mRNA of all seven sirtuins in the inner ear. In the immunohistochemical study, SIRT1-7 were abundant in many inner ear structures, i.e. stria vascularis, inner and outer hair cells, spiral ganglion cells, vestibular sensory and ganglion cells, vestibular dark and transitional cells, and the endolymphatic sac.
Department of Otolaryngology, Hiroshima University Hospital , Hiroshima , Japan.
Masaya
M
Takumida
Hiroshi
H
Takumida
Matti
M
Anniko
D000818:Animals; D007758:Ear, Inner / Q000166:cytology / Q000201:enzymology*; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D051379:Mice; D008808:Mice, Inbred CBA; D012333:RNA, Messenger / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D037761:Sirtuins / Q000096:biosynthesis / Q000235:genetics*; D041623:Tomography, Optical Coherence
D012333:RNA, Messenger; D037761:Sirtuins