Enantioselective surface plasmon resonance sensor based on C60 fullerene-glutathione self-assembled monolayer (SAM).
A novel enantioselective surface plasmon resonance (SPR) sensor based on a self-assembled monolayer of C60 fullerene as the chiral selector is proposed. A binding assay, apparent affinity constant, and apparent dissociation binding constant were used to analyze and study the enantioselectivity of C60 fullerene-glutathione film for L-histidine, which was chosen as the model analyte. The apparent affinity constant for the complex formed by L-histidine with C60 fullerene-glutathione film was 5.2 x 10(9)  M(-1). The proposed SPR sensor can be used for the assay of L-histidine in the 10(-10)-10(-7)  mol/L concentration range.
Laboratory of Electrochemistry and PATLAB Bucharest, National Institute of Research for Electrochemistry and Condensed Matter, Bucharest, Romania.
Raluca-Ioana
RI
Stefan-van Staden
D037741:Fullerenes / Q000737:chemistry*; D005978:Glutathione / Q000737:chemistry*; D006639:Histidine / Q000032:analysis / Q000737:chemistry; D013237:Stereoisomerism; D013379:Substrate Specificity; D020349:Surface Plasmon Resonance / Q000379:methods*
D037741:Fullerenes; D006639:Histidine; D005978:Glutathione; C069837:fullerene C60
C60 fullerene; L-histidine; enantioselective surface plasmon resonance sensor