Differential proteome and gene expression reveal response to carbon ion irradiation in pubertal mice testes.
Heavy ion radiation, a high linear energy transfer (LET) radiation, has been shown to have adverse effects on reproduction in male mice. The aim of this study was to profile and investigate the differentially expressed proteins in pubertal male mice testes following carbon ion radiation (CIR). Male mice underwent whole-body irradiation with CIR (1 and 4 Gy), and MALDI-TOF/TOF analysis was used to investigate the alteration in protein expression in 2-DE (two-dimensional gel electrophoresis) gels of testes caused by irradiation after 14 days. 8 differentially expressed proteins were identified and these proteins were mainly involved in energy supply, the endoplasmic reticulum, cell proliferation, cell cycle, antioxidant capacity and mitochondrial respiration, which play important roles in the inhibition of testicular function in response to CIR. Furthermore, we confirmed the relationship between transcription of mRNA and the abundance of proteins. Our results indicated that these proteins may lead to new insights into the molecular mechanism of CIR toxicity, and suggested that the gene expression response to CIR involves diverse regulatory mechanisms from transcription of mRNA to the formation of functional proteins.
Department of Heavy Ion Radiation Medicine, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Lanzhou 730000, China; Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province, Lanzhou 730000, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Hongyan
H
Li
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China.
Yuxuan
Y
He
Department of Heavy Ion Radiation Medicine, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Lanzhou 730000, China; Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province, Lanzhou 730000, China. Electronic address: zhangh@impcas.ac.cn.
Hong
H
Zhang
Department of Heavy Ion Radiation Medicine, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; University of Chinese Academy of Sciences, Beijing 100049, China; Department of Radiotherapy, Gansu Provincial Hospital, Lanzhou 730000, China.
Guoying
G
Miao
D000818:Animals; D002244:Carbon / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D005640:Follicle Stimulating Hormone / Q000097:blood*; D005786:Gene Expression Regulation / Q000528:radiation effects*; D063193:Heavy Ion Radiotherapy; D008297:Male; D051379:Mice; D040901:Proteomics / Q000379:methods; D012313:RNA / Q000737:chemistry / Q000235:genetics; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013737:Testis / Q000378:metabolism / Q000528:radiation effects*; D013739:Testosterone / Q000097:blood*; D014916:Whole-Body Irradiation
D013739:Testosterone; D012313:RNA; D002244:Carbon; D005640:Follicle Stimulating Hormone
Carbon ion radiation; Proteome; Pubertal mice; Testis