A novel GuttaFlow sealer supports cell survival and attachment.
BACKGROUND
The purpose of this in vitro study was to compare the biocompatibility of a novel formulation of a silicone-based endodontic sealer GuttaFlow 2 (GF2; Coltène/Whaledent, Langenau, Germany) with the original (GFO) and fast-set (GFF) formulations of GuttaFlow and with an epoxy resin sealer, AHPlus Jet (AH+J; Dentsply, York, PA).


METHODS
Sealers were set into 3 × 5.5 mm discs. Cell culture media was used to extract leachable products at 24 hours and 1, 2, and 4 weeks. Primary human periodontal ligament fibroblasts were incubated with sealer elutes for 24 hours and evaluated using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay and the calcein AM assay. Cell attachment was evaluated on set sealer that was either rinsed or unrinsed with cell media for 1 week. Statistical analysis was performed using the Student t test.


RESULTS
Both calcein and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assays revealed that periodontal ligament cell viability was reduced on AH+J at 1, 2, and 4 weeks compared with all GuttaFlow sealers. There were no differences in cell viability between the GuttaFlow samples, and all displayed high rates of cell survival at all time periods. After 2 hours, cell attachment to the rinsed GFO and GFF samples exceeded the control, and at 24 hours cell attachment on all GuttaFlow samples exceeded the control. AH+J sealers supported significantly less cell attachment when compared with all GuttaFlow sealers. Cell attachment to set sealers showed better cell attachment when rinsed compared with unrinsed.


CONCLUSIONS
GuttaFlow sealers were more biocompatible than AHJ in vitro. The novel GF2 displayed comparable biocompatibility with GFF and GFO.
Department of Endodontics, Louisiana State University Health Science Center, Center of Excellence in Oral and Craniofacial Biology, School of Dentistry, New Orleans, Louisiana.
Chelsea
C
Accardo
Department of Endodontics, Louisiana State University Health Science Center, Center of Excellence in Oral and Craniofacial Biology, School of Dentistry, New Orleans, Louisiana.
Van T
VT
Himel
Department of Cell Biology and Anatomy, Louisiana State University Health Science Center, Center of Excellence in Oral and Craniofacial Biology, School of Dentistry, New Orleans, Louisiana. Electronic address: tlalli@lsuhsc.edu.
Thomas E
TE
Lallier
D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004396:Coloring Agents; D003470:Culture Media; D004129:Dimethylpolysiloxanes / Q000737:chemistry / Q000494:pharmacology*; D004338:Drug Combinations; D004853:Epoxy Resins / Q000737:chemistry / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects*; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006171:Gutta-Percha / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D006813:Humidity; D008422:Materials Testing; D010513:Periodontal Ligament / Q000166:cytology* / Q000187:drug effects; D012387:Root Canal Filling Materials / Q000737:chemistry / Q000494:pharmacology*; D013696:Temperature; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors
D001672:Biocompatible Materials; D004396:Coloring Agents; D003470:Culture Media; D004129:Dimethylpolysiloxanes; D004338:Drug Combinations; D004853:Epoxy Resins; D005452:Fluoresceins; D005456:Fluorescent Dyes; C515718:GuttaFlow; D012387:Root Canal Filling Materials; D013778:Tetrazolium Salts; D013844:Thiazoles; C534916:epoxy resin-based root canal sealer; C085925:calcein AM; D006171:Gutta-Percha; C022616:thiazolyl blue
Cell; cell attachment; cell survival; culture; endodontic sealer; epoxy resin; silicone