Seven-valent pneumococcal conjugate vaccine and nasopharyngeal microbiota in healthy children.
Seven-valent pneumococcal conjugate vaccine (PCV-7) is effective against vaccine serotype disease and carriage. Nevertheless, shifts in colonization and disease toward nonvaccine serotypes and other potential pathogens have been described. To understand the extent of these shifts, we analyzed nasopharyngeal microbial profiles of 97 PCV-7-vaccinated infants and 103 control infants participating in a randomized controlled trial in the Netherlands. PCV-7 immunization resulted in a temporary shift in microbial community composition and increased bacterial diversity. Immunization also resulted in decreased presence of the pneumococcal vaccine serotype and an increase in the relative abundance and presence of nonpneumococcal streptococci and anaerobic bacteria. Furthermore, the abundance of Haemophilus and Staphylococcus bacteria in vaccinees was increased over that in controls. This study illustrates the much broader effect of vaccination with PCV-7 on the microbial community than currently assumed, and highlights the need for careful monitoring when implementing vaccines directed against common colonizers.
Giske
G
Biesbroek
Xinhui
X
Wang
Bart J F
BJ
Keijser
Rene M J
RM
Eijkemans
Krzysztof
K
Trzciński
Nynke Y
NY
Rots
Reinier H
RH
Veenhoven
Elisabeth A M
EA
Sanders
Debby
D
Bogaert
D002353:Carrier State; D002648:Child; D002675:Child, Preschool; D005260:Female; D006190:Haemophilus / Q000502:physiology; D006801:Humans; D007223:Infant; D008297:Male; D064307:Microbiota / Q000187:drug effects* / Q000276:immunology; D009305:Nasopharynx / Q000187:drug effects / Q000276:immunology / Q000382:microbiology*; D009426:Netherlands; D010802:Phylogeny; D011008:Pneumococcal Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D022242:Pneumococcal Vaccines / Q000008:administration & dosage / Q000276:immunology*; D012336:RNA, Ribosomal, 16S / Q000145:classification* / Q000235:genetics; D012703:Serotyping; D013210:Staphylococcus / Q000502:physiology; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000276:immunology*; D014611:Vaccination; D022223:Vaccines, Subunit
D022242:Pneumococcal Vaccines; D012336:RNA, Ribosomal, 16S; D022223:Vaccines, Subunit
PCV-7; bacteria; children; colonization; nasopharyngeal microbiota; pneumococcal conjugate vaccination; pneumococcal conjugate vaccine; pneumococci; randomized controlled trial; respiratory tract; seven-valent pneumococcal conjugate vaccine