Microtubule-dependent regulation of mitotic protein degradation.
Accurate cell division depends on tightly regulated ubiquitylation events catalyzed by the anaphase-promoting complex (APC/C). Among its many substrates, the APC/C triggers the degradation of proteins that stabilize the mitotic spindle, and loss or accumulation of such spindle assembly factors can result in aneuploidy and cancer. Although critical for cell division, it has remained poorly understood how the timing of spindle assembly factor degradation is established during mitosis. Here, we report that active spindle assembly factors are protected from APC/C-dependent degradation by microtubules. In contrast, those molecules that are not bound to microtubules are highly susceptible to proteolysis and turned over immediately after APC/C activation. The correct timing of spindle assembly factor degradation, as achieved by this regulatory circuit, is required for accurate spindle structure and function. We propose that the localized stabilization of APC/C substrates provides a mechanism for the selective disposal of cell-cycle regulators that have fulfilled their mitotic roles.
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Ling
L
Song
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Allison
A
Craney
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA. Electronic address: mrape@berkeley.edu.
Michael
M
Rape
D020816:Amino Acid Motifs; D064173:Anaphase-Promoting Complex-Cyclosome / Q000378:metabolism / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D008870:Microtubules / Q000378:metabolism / Q000502:physiology*; D009363:Neoplasm Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D008941:Spindle Apparatus / Q000378:metabolism* / Q000648:ultrastructure; D028961:beta Karyopherins / Q000378:metabolism / Q000502:physiology
C465041:DLGAP5 protein, human; D009363:Neoplasm Proteins; D028961:beta Karyopherins; D064173:Anaphase-Promoting Complex-Cyclosome