Evaluating the antimicrobial activity of methonolic extract of rhus succedanea leaf gall.
BACKGROUND
The worldwide increased bacterial resistance to antibiotics and the undesirable side effects associated with constant use of synthetic drugs has prompted the search for novel antimicrobial agents, particularly those manufactured from plants. This study is designed to ascertain the antibacterial potential of Rhus succedanea leaf gall extracts on the growth of gram-positive and gram-negative bacteria.


METHODS
The methanolic and hexane extract of different concentrations (100, 250, and 500 μg/ml) were prepared and their antibacterial efficacy was tested against clinical isolates of Escherichia coli, Salmonella typhi, Micrococcus luteus, and Staphylococcus aureus using agar well diffusion method and the size of inhibition zone was measured in millimeters.


RESULTS
The methanol and hexane extracts differed significantly in their antimicrobial activity with methanol extract showing a potent inhibitory activity in the range of 16±2 to 23±1, which was almost equal to the values of ciprofloxacin (25±3), used as a standard. Further, the methanol extract was mostly potent and effective in inhibiting the growth of gram-negative bacteria, namely, E. coli, when compared to gram -positive bacteria stains, which are responsible for antimicrobial activities. The phytochemical screening showed positive results for the presence of steroids, triterpenes, alkaloids, and carbohydrates.


CONCLUSIONS
The potent antibacterial activity of Rhus succedanea leaf gall extracts indicates its useful therapeutic application against bacterial infection. Furthermore, this study indicates that the extract might be exploited as natural drug for the treatment of infectious diseases and could be useful in understanding the relations between traditional cures and current medications.
Centre for Advanced studies in Biosciences, Jain University, Chamrajpete-560019, Bangalore, Karnataka, India.
Savitri
S
Shrestha
Centre for Advanced studies in Biosciences, Jain University, Chamrajpete-560019, Bangalore, Karnataka, India.
Sundara Rajan
SR
Subaramaihha
Centre for Advanced studies in Biosciences, Jain University, Chamrajpete-560019, Bangalore, Karnataka, India.
Sujan Ganapathy Pasura
SG
Subbaiah
Department of chemistry, School of graduate studies, Jain University, Bangalore-560002, India.
Ravi Shankara Birur
RS
Eshwarappa
Toxinology Lab, School of Chemical and Biotechnology (SCBT), SASTRA University, Tanjavur-613401, Tamilnadu, India ; Center for Emerging Technologies, Jain University, Ramanagara, Bangalore 562112, India.
Dhananjaya Bhadrapura
DB
Lakkappa
Antimicrobial; Drugs; Gall extracts; Phytochemicals; Rhus succedanea; Traditional medicine