Metabolite profiling of enzymatically hydrolyzed and fermented forms of Opuntia ficus-indica and their effect on UVB-induced skin photoaging.
Fermentation of natural products is emerging as an important processing method and is attracting a lot of attention because it may have the advantage of having a new biological function. In this study, fruits of Opuntia ficus-indica were enzymatically hydrolyzed and then fermented with two species of yeast. We identified novel prominent markers in enzymatically hydrolyzed O. ficus-indica (EO) and fermented O. ficus-indica (FO) samples by using an ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry. We also evaluated the effect of EO and FO on photoaging of skin cells exposed to ultraviolet radiation. We identified the major fermented metabolite in the FO as ferulic acid. Our in vitro study indicated that FO significantly enhanced the concentration of pro-collagen type 1 than the EO, by increasing the TGF-β1 production.
Sempio Fermentation Research Center, #183 Osongsaengmyeong 4ro, Osongeup, Cheongwongun, Chungcheongbukdo, 363-954, South Korea.
Dong-Woon
DW
Cho
Dae-Eung
DE
Kim
Dae-Hee
DH
Lee
Kyung-Hoon
KH
Jung
Byung-Serk
BS
Hurh
Oh Wook
OW
Kwon
Sun Yeou
SY
Kim
D001203:Ascomycota / Q000201:enzymology / Q000378:metabolism; D002478:Cells, Cultured; D002480:Cellulase / Q000378:metabolism; D024042:Collagen Type I / Q000378:metabolism; D003373:Coumaric Acids / Q000032:analysis / Q000378:metabolism; D055808:Drug Discovery; D005285:Fermentation; D005612:Freeze Drying; D005638:Fruit / Q000737:chemistry*; D005656:Fungal Proteins / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D029747:Opuntia / Q000737:chemistry*; D010843:Pichia / Q000201:enzymology / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D028321:Plant Preparations / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D011347:Procollagen / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D015595:Skin Aging / Q000187:drug effects*; D013473:Sunscreening Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D014242:Trichoderma / Q000201:enzymology; D014466:Ultraviolet Rays / Q000009:adverse effects
D024042:Collagen Type I; D003373:Coumaric Acids; D005656:Fungal Proteins; D010936:Plant Extracts; D028321:Plant Preparations; D011347:Procollagen; D013473:Sunscreening Agents; C004999:ferulic acid; D002480:Cellulase