A multi-spectroscopic approach to investigate the interaction of prodigiosin with ct-DNA.
The interaction between prodigiosin (PG) and calf thymus DNA (ct-DNA) was investigated firstly by using UV-Visible (UV-Vis), fluorescence, Fourier transform infrared (FT-IR), circular dichroism (CD) spectroscopies and viscosity measurement in Tris-HCl buffer solution (pH 6.8). The experimental results indicated that PG intercalated into the DNA helix. Upon addition of ct-DNA, PG showed hypochromic effect and slight redshift in the absorption spectra, and the melting temperature of ct-DNA was increased by from 58 to 64°C. Furthermore, FT-IR spectrum and CD spectra also suggested that the partial bases of ct-DNA react with prodigiosin. The fluorescence quenching mechanism was studied using ethidium bromide as a DNA probe, The binding constants of PG with ct-DNA in the presence of EB are 4.46×10(4) and 2.32×10(4)M(-1) at 298 and 310K, respectively, and the corresponding thermodynamic parameters ΔG, ΔH, ΔS at various temperatures were obtained.
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Biomedical Materials, College of Chemistry and Materials Science, Analysis and Testing Center, Key Laboratory of Applied Photochemistry, Nanjing Normal University, Nanjing 210023, China.
Lina
L
Han
Department of Physical Science and Technology, Nanjing Normal University, Nanjing 210023, China.
Yanhuai
Y
Zhou
Department of Physical Science and Technology, Nanjing Normal University, Nanjing 210023, China.
Xiaoqin
X
Huang
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Biomedical Materials, College of Chemistry and Materials Science, Analysis and Testing Center, Key Laboratory of Applied Photochemistry, Nanjing Normal University, Nanjing 210023, China.
Mengsi
M
Xiao
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Biomedical Materials, College of Chemistry and Materials Science, Analysis and Testing Center, Key Laboratory of Applied Photochemistry, Nanjing Normal University, Nanjing 210023, China.
Lin
L
Zhou
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Biomedical Materials, College of Chemistry and Materials Science, Analysis and Testing Center, Key Laboratory of Applied Photochemistry, Nanjing Normal University, Nanjing 210023, China. Electronic address: zhoujiahong@njnu.edu.cn.
Jiahong
J
Zhou
Nursing College of Nanjing University of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. Electronic address: aihwang@163.com.
Aihong
A
Wang
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Biomedical Materials, College of Chemistry and Materials Science, Analysis and Testing Center, Key Laboratory of Applied Photochemistry, Nanjing Normal University, Nanjing 210023, China.
Jian
J
Shen
D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004996:Ethidium / Q000737:chemistry; D007364:Intercalating Agents / Q000378:metabolism*; D009690:Nucleic Acid Conformation / Q000187:drug effects; D011353:Prodigiosin / Q000378:metabolism*; D011485:Protein Binding; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
D000900:Anti-Bacterial Agents; D007364:Intercalating Agents; D004247:DNA; C427203:calf thymus DNA; D004996:Ethidium; D011353:Prodigiosin
Ct-DNA; Ethidium bromide (EB); Fluorescence quenching; Intercalation; Prodigiosin