[Effect of titanium particles and TNF-alpha on the gene expression and activity of MMP-1, 2, 3 in human knee joint synovial cells].
This paper is aimed to investigate the effect of titanium (Ti) particles and tumor necrosis factor alpha (TNF-alpha) on the expressions of MMP-1, 2, 3 in human synovial cells, so as to explore the possible mechanism of osteolysis post-operation of metal-on-metal total joint arthroplasty in human synovial cells induced by Ti particles. In vitro cell cultures, human synovial cells were treated by Ti particles and/or TNF-alpha. The total RNA was isolated at 2 hours after the treatment. The gene expression of MMP-1, 2, 3 was analyzed by Semi-quantitative Reverse-transcriptional PCR and quantitative real-time PCR. Cell supernatant was collected at 12, 24, 48 hours after the treatment and Gelatin zymography was performed to detect the activity of MMP-2. Compared to those in the control group (untreated), Ti particles and TNF-alpha increased the gene expression of MMP-1, 2, 3 respectively (P < 0.05), and the effect of combination of the two was even more significant (P < 0.01). The trend of activities of MMP-2 is similar with gene expression. Ti particles and TNF-alpha increased MMP-2 activities by 1.3 times and 1.5 times respectively (P < 0.05), and the combination of the two increased by 1.7 times (P < 0.01). Ti particles and TNF-alpha-induced the stimulation of MMP-1, 2, 3 expressions and MMP-2 activities in human knee joint synovial cells may be involved in aseptic loosening after metal-on-metal arthroplasty through increasing the degradation of bone matrix and declining of osseous support structure mechanics.
Department of Orthopaedics, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
Chunfeng
C
Fu
College of Bioengineering, Chongqing University, Chongqing 400044, China.
Jing
J
Xie
Department of Orthopaedics, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
Rongfu
R
Chen
College of Bioengineering, Chongqing University, Chongqing 400044, China.
Chunli
C
Wang
College of Bioengineering, Chongqing University, Chongqing 400044, China.
Chunming
C
Xu
Department of Orthopaedics, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
Cheng
C
Chen
Department of Orthopaedics, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
Zhiqiang
Z
Wang
Department of Orthopaedics, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
Liangbo
L
Lin
Department of Orthopaedics, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
Wei
W
Huang
Department of Orthopaedics, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
Xi
X
Liang
College of Bioengineering, Chongqing University, Chongqing 400044, China.
K L Paul
KL
Sung
D002478:Cells, Cultured; D006801:Humans; D007595:Joint Prosthesis; D007719:Knee Joint / Q000166:cytology*; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D019278:Matrix Metalloproteinase 3 / Q000235:genetics / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism*; D010316:Particle Size; D011475:Prosthesis Failure / Q000009:adverse effects; D012313:RNA / Q000235:genetics / Q000378:metabolism; D013583:Synovial Membrane / Q000166:cytology / Q000201:enzymology*; D014025:Titanium / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D014409:Tumor Necrosis Factor-alpha; D012313:RNA; D014025:Titanium; D020782:Matrix Metalloproteinases; C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1