Tracheal reconstruction using chondrocytes seeded on a poly(L-lactic-co-glycolic acid)-fibrin/hyaluronan.
Reconstruction of trachea is still a clinical dilemma. Tissue engineering is a recent and promising concept to resolve this problem. This study evaluated the feasibility of allogeneic chondrocytes cultured with fibrin/hyaluronic acid (HA) hydrogel and degradable porous poly(L-lactic-co-glycolic acid) (PLGA) scaffold for partial tracheal reconstruction. Chondrocytes from rabbit articular cartilage were expanded and cultured with fibrin/HA hydrogel and injected into a 5 × 10 mm-sized, curved patch-shape PLGA scaffold. After 4 weeks in vitro culture, the scaffold was implanted on a tracheal defect in eight rabbits. Six and 10 weeks postoperatively, the implanted sites were evaluated by bronchoscope and radiologic and histologic analyses. Ciliary beat frequency (CBF) of regenerated epithelium was also evaluated. None of the eight rabbits showed any sign of respiratory distress. Bronchoscopic examination did not reveal stenosis of the reconstructed trachea and the defects were completely recovered with respiratory epithelium. Computed tomography scan showed good luminal contour of trachea. Histologic data showed that the implanted chondrocytes successfully formed neocartilage with minimal granulation tissue. CBF of regenerated epithelium was similar to that of normal epithelium. Partial tracheal defect was successfully reconstructed anatomically and functionally using allogeneic chondrocytes cultured with PLGA-fibrin/HA composite scaffold.
Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Republic of Korea.
Hyun Jun
HJ
Hong
Jae Won
JW
Chang
Ju-Kyeong
JK
Park
Jae Won
JW
Choi
Yoo Suk
YS
Kim
Yoo Seob
YS
Shin
Chul-Ho
CH
Kim
Eun Chang
EC
Choi
D064591:Allografts; D000818:Animals; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism / Q000637:transplantation; D005337:Fibrin / Q000494:pharmacology*; D006820:Hyaluronic Acid / Q000494:pharmacology*; D019344:Lactic Acid / Q000494:pharmacology*; D011100:Polyglycolic Acid / Q000494:pharmacology*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011817:Rabbits; D012038:Regeneration; D020545:Respiratory Mucosa / Q000166:cytology / Q000378:metabolism; D054457:Tissue Scaffolds; D014132:Trachea / Q000601:surgery*
D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D005337:Fibrin; D006820:Hyaluronic Acid
chondrocyte; fibrin; hyaluronan; poly(l-lactic-co-glycolic acid); tracheal regeneration