The role of cytochrome P450 2B6 and 2B4 substrate access channel residues predicted based on crystal structures of the amlodipine complexes.
Recent X-ray crystal structures of human cytochrome P450 2B6 and rabbit cytochrome P450 2B4 in complex with amlodipine showed two bound ligand molecules, one in the active site and one in the substrate access channel. Based on the X-ray crystal structures, we investigated the interactions of P450 2B4 and 2B6 with amlodipine using absorbance spectroscopy, and determined the steady-state kinetics of 7-ethoxy-4-(trifluoromethyl)coumarin and 7-benzyloxyresorufin oxidation by some access channel mutants to evaluate the functional role of these residues in substrate turnover. The results of absorbance titrations are consistent with a simple mechanism with two parallel binding events that result in the formation of the enzyme complex with two molecules of amlodipine. Using this model we were able to resolve two separate ligand-binding events, which are characterized by two distinct KD values in each enzyme. The access channel mutants R73K in P450 2B6 and R73K, V216W, L219W, and F220W in P450 2B4 showed a significant decrease in kcat/KM with the both substrates. Overall, the results suggest that P450 2B4 and 2B6 form an enzyme complex with two molecules of amlodipine in solution, and R73, V216, L219 and F220 in P450 2B4 may play an important role in substrate metabolism.
Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, CA 92093, United States. Electronic address: hyj006@ucsd.edu.
Hyun-Hee
HH
Jang
Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, CA 92093, United States.
Dmitri R
DR
Davydov
School of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea.
Ga-Young
GY
Lee
School of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea.
Chul-Ho
CH
Yun
Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, CA 92093, United States.
James R
JR
Halpert
D017311:Amlodipine / Q000378:metabolism*; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D002121:Calcium Channel Blockers / Q000378:metabolism*; D020134:Catalytic Domain; D003374:Coumarins / Q000378:metabolism; D018360:Crystallography, X-Ray; D065702:Cytochrome P-450 CYP2B6; D000072467:Cytochrome P450 Family 2; D006801:Humans; D008958:Models, Molecular; D017354:Point Mutation; D011487:Protein Conformation; D011817:Rabbits
D002121:Calcium Channel Blockers; D003374:Coumarins; D017311:Amlodipine; C030123:coumarin; D001189:Aryl Hydrocarbon Hydroxylases; C585599:CYP2B6 protein, human; D065702:Cytochrome P-450 CYP2B6; D000072467:Cytochrome P450 Family 2; C465241:cytochrome P-450 CYP2B4 (rabbit)
Amlodipine; Cytochrome P450 2B4 and 2B6; Spectral titrations; Substrate access channel 2f and 2a