Lupane triterpenoids from the stems of Euonymus carnosus.
Fifteen new lupane-type triterpenoids (1-15) and 10 known triterpenoids (16-25) were isolated from the stems of Euonymus carnosus. The structures of the new compounds were elucidated on the basis of spectroscopic analyses, and the absolute configuration of compound 1 was confirmed by X-ray crystallographic analysis using anomalous scattering of Cu Kα radiation. In addition, the compounds were tested for their cytotoxic activity against five human cancer cell lines and their ability to inhibit LPS-induced nitric oxide production in the murine microglia BV2 cell line. Compound 11 exhibited moderate cytotoxicity against several human cancer cell lines, and compounds 1, 2, 4, 5, 20, and 25 showed neuritis inhibitory activity against microglial inflammation factor, with IC50 values of 7.39, 7.48, 7.80, 3.48, 2.54, and 6.09 μM, respectively.
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing 100050, People's Republic of China.
Jian
J
Zhou
Chuang-Jun
CJ
Li
Jing-Zhi
JZ
Yang
Jie
J
Ma
Yan
Y
Li
Xiu-Qi
XQ
Bao
Xiao-Guang
XG
Chen
Dan
D
Zhang
Dong-Ming
DM
Zhang
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D018360:Crystallography, X-Ray; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D029756:Euonymus / Q000737:chemistry*; D006801:Humans; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D017628:Microglia / Q000187:drug effects; D008968:Molecular Conformation; D015394:Molecular Structure; D009569:Nitric Oxide / Q000096:biosynthesis; D019906:Nuclear Magnetic Resonance, Biomolecular; D018547:Plant Stems / Q000737:chemistry; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D053978:Pentacyclic Triterpenes
D000893:Anti-Inflammatory Agents; D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D008070:Lipopolysaccharides; D009569:Nitric Oxide; D014315:Triterpenes; C480546:lupane; D053978:Pentacyclic Triterpenes