Self-assembled monolayer facilitates epithelial-mesenchymal interactions mimicking odontogenesis.
Cell-cell interactions are vital for embryonic organ development and normal function of differentiated cells and tissues. In this study we have developed a self-assembled monolayer-based co-culture system to study tooth morphogenesis. Specifically, we designed a 2-D microenvironment present in the dental tissue by creating a well-structured, laterally organized epithelial and mesenchymal cell co-culture system by patterning the cell-attachment substrate. Chemical modifications were used to develop tunable surface patterns to facilitate epithelial-mesenchymal interactions mimicking the developing tooth. Such a design promoted interactions between monolayer's of the 2 cell types and provided signaling cues that resulted in cellular differentiation and mineralized matrix formation. Gene expression analysis showed that these co-cultures mimicked in-vivo conditions than monolayer cultures of a single cell type.
Brodie Tooth Development Genetics & Regenerative Medicine Research Laboratory, Department of Oral Biology, University of Illinois at Chicago , Chicago, IL , USA and.
Tanvi
T
Muni
Milan
M
Mrksich
Anne
A
George
D000327:Adsorption; D000473:Alkanes / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D018920:Coculture Techniques; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D008648:Mesoderm / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009805:Odontogenesis / Q000187:drug effects*; D051381:Rats
D000473:Alkanes; D005353:Fibronectins; D016650:Fluorescein-5-isothiocyanate