Mitochondrial genome of the stonefly Kamimuria wangi (Plecoptera: Perlidae) and phylogenetic position of plecoptera based on mitogenomes.
This study determined the mitochondrial genome sequence of the stonefly, Kamimuria wangi. In order to investigate the relatedness of stonefly to other members of Neoptera, a phylogenetic analysis was undertaken based on 13 protein-coding genes of mitochondrial genomes in 13 representative insects. The mitochondrial genome of the stonefly is a circular molecule consisting of 16,179 nucleotides and contains the 37 genes typically found in other insects. A 10-bp poly-T stretch was observed in the A+T-rich region of the K. wangi mitochondrial genome. Downstream of the poly-T stretch, two regions were located with potential ability to form stem-loop structures; these were designated stem-loop 1 (positions 15848-15651) and stem-loop 2 (15965-15998). The arrangement of genes and nucleotide composition of the K. wangi mitogenome are similar to those in Pteronarcys princeps, suggesting a conserved genome evolution within the Plecoptera. Phylogenetic analysis using maximum likelihood and Bayesian inference of 13 protein-coding genes supported a novel relationship between the Plecoptera and Ephemeroptera. The results contradict the existence of a monophyletic Plectoptera and Plecoptera as sister taxa to Embiidina, and thus requires further analyses with additional mitogenome sampling at the base of the Neoptera.
School of Horticulture and Plant Protection and Institute of Applied Entomology, Yangzhou University, Yangzhou, Jiangsu, China ; College of Forestry, Southwest Forestry University, Kunming, Yunnan, China.
Qian
Q
Yu-Han
School of Horticulture and Plant Protection and Institute of Applied Entomology, Yangzhou University, Yangzhou, Jiangsu, China.
Wu
W
Hai-Yan
School of Horticulture and Plant Protection and Institute of Applied Entomology, Yangzhou University, Yangzhou, Jiangsu, China.
Ji
J
Xiao-Yu
School of Horticulture and Plant Protection and Institute of Applied Entomology, Yangzhou University, Yangzhou, Jiangsu, China.
Yu
Y
Wei-Wei
School of Horticulture and Plant Protection and Institute of Applied Entomology, Yangzhou University, Yangzhou, Jiangsu, China.
Du
D
Yu-Zhou
D000818:Animals; D003062:Codon; D017344:Genes, Insect; D054629:Genome, Mitochondrial; D007313:Insecta / Q000145:classification / Q000235:genetics*; D055029:Inverted Repeat Sequences; D058977:Molecular Sequence Annotation; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D012343:RNA, Transfer / Q000235:genetics; D017422:Sequence Analysis, DNA
D003062:Codon; D012343:RNA, Transfer