Chronic supplementation of proanthocyanidins reduces postprandial lipemia and liver miR-33a and miR-122 levels in a dose-dependent manner in healthy rats.
Elevated postprandial triglycerides are associated with an increased risk of cardiovascular disease. Acute proanthocyanidin supplementation improves postprandial lipemia. Therefore, in this study, we evaluated whether a chronic treatment (3 weeks) of grape seed proanthocyanidins (GSPE) improves tolerance to lipid overload and represses liver microRNA (miRNA)-33a and miRNA-122 and their target genes as a mechanism to soften the elevated postprandial triglycerides in healthy rats. Additionally, the minimal GSPE chronic dose required to alter miRNA levels was determined by means of a dose-response experiment using 5, 15, 25 or 50 mg of GSPE/kg body weight. GSPE repressed miR-33a and miR-122 liver expression and reduced postprandial lipemia in a dose-dependent manner. Significant effects were only observed at high levels of proanthocyanidin consumption, but moderate doses of proanthocyanidins were still able to modulate miRNA expression. Therefore, it can be suggested that a population with a normal intake of proanthocyanidin-rich foods can benefit from the modulation of miRNA expression. At the molecular level, this action can confer homeostatic robustness and will thus exert subtle changes in lipid metabolism, thereby reducing the risk associated with postprandial hyperlipemia.
Department of Biochemistry and Biotechnology, Universitat Rovira i Virgili, C/Marcel.lí Domingo s/n, 43007 Tarragona, Spain.
Laura
L
Baselga-Escudero
Department of Biochemistry and Biotechnology, Universitat Rovira i Virgili, C/Marcel.lí Domingo s/n, 43007 Tarragona, Spain. Electronic address: mariacinta.blade@urv.cat.
Cinta
C
Blade
Department of Biochemistry and Biotechnology, Universitat Rovira i Virgili, C/Marcel.lí Domingo s/n, 43007 Tarragona, Spain.
Aleix
A
Ribas-Latre
Department of Biochemistry and Biotechnology, Universitat Rovira i Virgili, C/Marcel.lí Domingo s/n, 43007 Tarragona, Spain.
Ester
E
Casanova
Department of Biochemistry and Biotechnology, Universitat Rovira i Virgili, C/Marcel.lí Domingo s/n, 43007 Tarragona, Spain.
M-Josepa
MJ
Salvadó
Department of Biochemistry and Biotechnology, Universitat Rovira i Virgili, C/Marcel.lí Domingo s/n, 43007 Tarragona, Spain.
Lluis
L
Arola
Department of Biochemistry and Biotechnology, Universitat Rovira i Virgili, C/Marcel.lí Domingo s/n, 43007 Tarragona, Spain.
Anna
A
Arola-Arnal
D000818:Animals; D004305:Dose-Response Relationship, Drug; D006949:Hyperlipidemias / Q000517:prevention & control*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D019518:Postprandial Period; D044945:Proanthocyanidins / Q000008:administration & dosage* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction
C580643:MIRN122 microRNA, rat; C580642:MIRN33 microRNA, rat; D035683:MicroRNAs; D044945:Proanthocyanidins
Abca1; Cholesterol; Fas; Flavonoids; Triglycerides; microRNAs