Immune response elicited by DNA vaccination using Lactococcus lactis is modified by the production of surface exposed pathogenic protein.
In this study, we compared immune responses elicited by DNA immunization using Lactococcus lactis or L. lactis expressing the Staphylococcus aureus invasin Fibronectin Binding Protein A (FnBPA) at its surface. Both strains carried pValac:BLG, a plasmid containing the cDNA of Beta-Lactoglobulin (BLG), and were designated LL-BLG and LL-FnBPA+ BLG respectively. A TH2 immune response characterized by the secretion of IL-4 and IL-5 in medium of BLG reactivated splenocytes was detected after either oral or intranasal administration of LL-FnBPA+ BLG. In contrast, intranasal administration of LL-BLG elicited a TH1 immune response. After BLG sensitization, mice previously intranasally administered with LL-BLG showed a significantly lower concentration of BLG-specific IgE than the mice non-administered. Altenatively administration of LL-FnBPA+ BLG didn't modify the BLG-specific IgE concentration obtained after sensitization, thus confirming the TH2 orientation of the immune response. To determine if the TH2-skewed immune response obtained with LL-FnBpA+ BLG was FnBPA-specific or not, mice received another L. lactis strain producing a mutated form of the Listeria monocytogenes invasin Internalin A intranasally, allowing thus the binding to murine E-cadherin, and containing pValac:BLG (LL-mInlA+ BLG). As with LL-FnBPA+ BLG, LL-mInlA+ BLG was not able to elicit a TH1 immune response. Furthermore, we observed that these difference were not due to the peptidoglycan composition of the cell wall as LL-FnBPA+ BLG, LL-mInlA+ BLG and LL-BLG strains shared a similar composition. DNA vaccination using LL-BLG elicited a pro-inflammatory TH1 immune response while using LL-FnBPA+ BLG or LL-mInlA+ BLG elicited an anti-inflammatory TH2 immune response.
State University of Paraiba, Campus V, Department of Biological Sciences. João Pessoa, Paraíba, Brazil ; INRA, UMR1319 Micalis, Domaine de Vilvert, Jouy-en-Josas, France ; AgroParisTech, UMR1319 Micalis, Jouy-en-Josas, France.
Daniela
D
Pontes
INRA, UMR1319 Micalis, Domaine de Vilvert, Jouy-en-Josas, France ; AgroParisTech, UMR1319 Micalis, Jouy-en-Josas, France ; Institute of Biological Sciences, Federal University of Minas Gerais (UFMG-ICB), Belo Horizonte, Minas Gerais, Brazil.
Marcela
M
Azevedo
INRA, UMR1319 Micalis, Domaine de Vilvert, Jouy-en-Josas, France ; AgroParisTech, UMR1319 Micalis, Jouy-en-Josas, France ; Lymphocyte Signaling and Development Laboratory, Babraham Institute, Babraham Research Campus, Cambridge, United Kingdom.
Silvia
S
Innocentin
INRA, UMR1319 Micalis, Domaine de Vilvert, Jouy-en-Josas, France ; AgroParisTech, UMR1319 Micalis, Jouy-en-Josas, France.
Sébastien
S
Blugeon
INRA, VIM, Domaine de Vilvert, Jouy-en-Josas, France.
François
F
Lefévre
Institute of Biological Sciences, Federal University of Minas Gerais (UFMG-ICB), Belo Horizonte, Minas Gerais, Brazil.
Vasco
V
Azevedo
Institute of Biological Sciences, Federal University of Minas Gerais (UFMG-ICB), Belo Horizonte, Minas Gerais, Brazil.
Anderson
A
Miyoshi
INRA, UMR1319 Micalis, Domaine de Vilvert, Jouy-en-Josas, France ; AgroParisTech, UMR1319 Micalis, Jouy-en-Josas, France.
Pascal
P
Courtin
INRA, UMR1319 Micalis, Domaine de Vilvert, Jouy-en-Josas, France ; AgroParisTech, UMR1319 Micalis, Jouy-en-Josas, France.
Marie-Pierre
MP
Chapot-Chartier
INRA, UMR1319 Micalis, Domaine de Vilvert, Jouy-en-Josas, France ; AgroParisTech, UMR1319 Micalis, Jouy-en-Josas, France.
Philippe
P
Langella
INRA, UMR1319 Micalis, Domaine de Vilvert, Jouy-en-Josas, France ; AgroParisTech, UMR1319 Micalis, Jouy-en-Josas, France.
Jean-Marc
JM
Chatel
D018829:Adhesins, Bacterial / Q000235:genetics / Q000276:immunology; D000281:Administration, Intranasal; D000284:Administration, Oral; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology; D060846:Cell Engineering; D016503:Drug Delivery Systems / Q000379:methods; D005260:Female; D007110:Immunity, Active; D007111:Immunity, Cellular; D013294:Lactococcus lactis / Q000235:genetics* / Q000276:immunology; D007782:Lactoglobulins / Q000235:genetics / Q000276:immunology; D008088:Listeriosis / Q000276:immunology / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D013203:Staphylococcal Infections / Q000276:immunology / Q000517:prevention & control*; D018417:Th1 Cells / Q000166:cytology / Q000276:immunology; D018418:Th2 Cells / Q000166:cytology / Q000276:immunology; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000096:biosynthesis / Q000235:genetics; D014614:Vaccines, Synthetic
D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D007782:Lactoglobulins; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic; C078635:fibronectin-binding proteins, bacterial; C069761:internalin protein, Bacteria