Easily controlled grafting of oligonucleotides on γFe2O3 nanoparticles: physicochemical characterization of DNA organization and biological activity studies.
We report a one-step process to functionalize superparamagnetic iron oxide nanoparticle (SPIO-NP) surfaces with a controlled number of oligonucleotides. For this study, we use a specific oligonucleotide targeting the signal transducer and activator of transcription 3 (STAT3), a key regulator of cell survival and proliferation. This oligonucleotide is self-complementary and can adopt a hairpin structure. It is labeled with the fluorescein amidite group at the 3'-end. The polyanionic DNA is electrostatically attracted onto the positively charged surface of the bare SPIO-NPs. During synthesis, the molar ratio between the oligonucleotides and nanoparticles was varied from 17.5 to 175. For particles with a mean diameter of 10 nm, a nanoparticle surface saturation is observed corresponding to 70 DNA strands per particle. The increase of DNA density per nanoparticle is correlated to a transition from the hairpin structure adsorbed horizontally on the nanoparticle surface to a vertically ordered surface packing assembly. An in vitro study on human colon carcinoma cell line SW480 shows that the kinetics of internalization and biological activity of the NPs seem to be dependent on the oligonucleotide density. Cell death and the kinetics of internalization are favored by a low density of oligonucleotides.
Université Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, CNRS , (UMR 7244), F-93017, Bobigny, France.
Frédéric
F
Geinguenaud
Inès
I
Souissi
Rémi
R
Fagard
Yoann
Y
Lalatonne
Laurence
L
Motte
D001483:Base Sequence; D001692:Biological Transport; D045744:Cell Line, Tumor; D055598:Chemical Phenomena; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D004355:Drug Stability; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D057446:Hydrodynamics; D053758:Nanoparticles / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D013499:Surface Properties
D005290:Ferric Compounds; D009841:Oligonucleotides; C000499:ferric oxide; D004247:DNA