Both dietary supplementation with monosodium L-glutamate and fat modify circulating and tissue amino acid pools in growing pigs, but with little interactive effect.
BACKGROUND
The Chinese population has undergone rapid transition to a high-fat diet. Furthermore, monosodium L-glutamate (MSG) is widely used as a daily food additive in China. Little information is available on the effects of oral MSG and dietary fat supplementation on the amino acid balance in tissues. The present study aimed to determine the effects of both dietary fat and MSG on amino acid metabolism in growing pigs, and to assess any possible interactions between these two nutrients.


RESULTS
Four iso-nitrogenous and iso-caloric diets (basal diet, high fat diet, basal diet with 3% MSG and high fat diet with 3% MSG) were provided to growing pigs. The dietary supplementation with fat and MSG used alone and in combination were found to modify circulating and tissue amino acid pools in growing pigs. Both dietary fat and MSG modified the expression of gene related to amino acid transport in jejunum.


CONCLUSIONS
Both dietary fat and MSG clearly influenced amino acid content in tissues but in different ways. Both dietary fat and MSG enhance the absorption of amino acids in jejunum. However, there was little interaction between the effects of dietary fat and MSG.
Institute of Subtropical Agriculture, Chinese Academy of Sciences, Research Center of Healthy Breeding Livestock & Poultry, Hunan Engineering & Research Center of Animal & Poultry Science, Key Lab Agro-ecology Processing Subtropical Region, Scientific observational and experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan, Peoples Republic of China.
Zemeng
Z
Feng
College of Food and Pharmacy Engineering, Guiyang University, Guiyang, Guizhou, Peoples Republic of China.
Xiaoli
X
Zhou
Institute of Subtropical Agriculture, Chinese Academy of Sciences, Research Center of Healthy Breeding Livestock & Poultry, Hunan Engineering & Research Center of Animal & Poultry Science, Key Lab Agro-ecology Processing Subtropical Region, Scientific observational and experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan, Peoples Republic of China.
Fei
F
Wu
Institute of Subtropical Agriculture, Chinese Academy of Sciences, Research Center of Healthy Breeding Livestock & Poultry, Hunan Engineering & Research Center of Animal & Poultry Science, Key Lab Agro-ecology Processing Subtropical Region, Scientific observational and experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan, Peoples Republic of China.
Kang
K
Yao
Institute of Subtropical Agriculture, Chinese Academy of Sciences, Research Center of Healthy Breeding Livestock & Poultry, Hunan Engineering & Research Center of Animal & Poultry Science, Key Lab Agro-ecology Processing Subtropical Region, Scientific observational and experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan, Peoples Republic of China.
Xiangfeng
X
Kong
Institute of Subtropical Agriculture, Chinese Academy of Sciences, Research Center of Healthy Breeding Livestock & Poultry, Hunan Engineering & Research Center of Animal & Poultry Science, Key Lab Agro-ecology Processing Subtropical Region, Scientific observational and experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan, Peoples Republic of China.
Tiejun
T
Li
Nutrition Physiology and Ingestive Behavior, Paris Institute of Technology for Life Food and Environmental Sciences, Paris, France.
Francois
F
Blachier
Institute of Subtropical Agriculture, Chinese Academy of Sciences, Research Center of Healthy Breeding Livestock & Poultry, Hunan Engineering & Research Center of Animal & Poultry Science, Key Lab Agro-ecology Processing Subtropical Region, Scientific observational and experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan, Peoples Republic of China.
Yulong
Y
Yin
D000596:Amino Acids / Q000097:blood*; D000818:Animals; D059305:Diet, High-Fat; D004041:Dietary Fats / Q000008:administration & dosage*; D005260:Female; D005503:Food Additives / Q000008:administration & dosage*; D015870:Gene Expression; D007583:Jejunum / Q000378:metabolism; D008297:Male; D044169:Receptors, Calcium-Sensing / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D012970:Sodium Glutamate / Q000008:administration & dosage*; D013552:Swine / Q000097:blood*
D000596:Amino Acids; D004041:Dietary Fats; D005503:Food Additives; D044169:Receptors, Calcium-Sensing; D043562:Receptors, G-Protein-Coupled; C431926:taste receptors, type 1; D012970:Sodium Glutamate