A novel approach for skin rejuvenation by regenerative medicine: delivery of stem cell-derived growth factors through an iontophoretic system.
OBJECTIVE
It was hypothesized that mesenchymal stem cell (MSC)-derived growth factor applied by iontophoretic transdermal delivery might improve ultraviolet beam (UVB)-induced photoaged skin. This experimental study was performed to confirm this hypothesis.


METHODS
Photoaged skin was induced in nude rat after an 8-week regimen of UVB irradiation, and an MSC-conditioned medium (MSC-CM) was conducted by an iontophoretic transdermal transport system. The effect of iontophoresis with MSC-CM was evaluated by replica method and measurement of the hyaluronic acid level in the dermis after treatment.


RESULTS
MSC-CM conducted by iontophoresis significantly reduced all parameters for wrinkles and increased hyaluronic acid in the dermis.


CONCLUSIONS
MSC-CM application by iontophoresis can be considered as a supplemental treatment to the methods for skin rejuvenation.
Minoru
M
Ueda
D000279:Administration, Cutaneous; D000818:Animals; D017077:Culture Media, Conditioned; D006820:Hyaluronic Acid / Q000032:analysis* / Q000096:biosynthesis; D036341:Intercellular Signaling Peptides and Proteins / Q000008:administration & dosage* / Q000378:metabolism; D007478:Iontophoresis / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051381:Rats; D011923:Rats, Nude; D012060:Rejuvenation; D012867:Skin / Q000473:pathology / Q000528:radiation effects; D015595:Skin Aging / Q000473:pathology; D014466:Ultraviolet Rays / Q000009:adverse effects
D017077:Culture Media, Conditioned; D036341:Intercellular Signaling Peptides and Proteins; D006820:Hyaluronic Acid