In vitro studies on the relationship between the antioxidant activities of some berry extracts and their binding properties to serum albumin.
The aim of this study was to investigate the possibility to use the bioactive components from cape gooseberry (Physalis peruviana), blueberry (Vaccinium corymbosum), and cranberry (Vaccinium macrocarpon) extracts as a novel source against oxidation in food supplementation. The quantitative analysis of bioactive compounds (polyphenols, flavonoids, flavanols, carotenoids, and chlorophyll) was based on radical scavenging spectrophometric assays and mass spectrometry. The total phenolic content was the highest (P < 0.05) in water extract of blueberries (46.6 ± 4.2 mg GAE/g DW). The highest antioxidant activities by 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay and Cupric reducing antioxidant capacity were in water extracts of blueberries, showing 108.1 ± 7.2 and 131.1 ± 9.6 μMTE/g DW with correlation coefficients of 0.9918 and 0.9925, and by β-carotene linoleate assay at 80.1 ± 6.6 % with correlation coefficient of 0.9909, respectively. The water extracts of berries exhibited high binding properties with human serum albumin in comparison with quercetin. In conclusion, the bioactive compounds from a relatively new source of gooseberries in comparison with blueberries and cranberries have the potential as food supplementation for human health. The antioxidant and binding activities of berries depend on their bioactive compounds.
Department of Analytical Chemistry, Chemical Faculty, Gdańsk University of Technology, 80952, Gdańsk, Poland, chemanal@pg.gda.pl.
Jacek
J
Namiesnik
Kann
K
Vearasilp
Alina
A
Nemirovski
Hanna
H
Leontowicz
Maria
M
Leontowicz
Pawel
P
Pasko
Alma Leticia
AL
Martinez-Ayala
Gustavo A
GA
González-Aguilar
Milan
M
Suhaj
Shela
S
Gorinstein
D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors; D002338:Carotenoids / Q000737:chemistry / Q000302:isolation & purification; D002734:Chlorophyll / Q000737:chemistry / Q000302:isolation & purification; D019587:Dietary Supplements; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D010084:Oxidation-Reduction; D032321:Physalis / Q000737:chemistry*; D010851:Picrates / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000737:chemistry*; D059808:Polyphenols / Q000737:chemistry / Q000302:isolation & purification; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry*; D029796:Vaccinium / Q000737:chemistry*; D029799:Vaccinium macrocarpon / Q000737:chemistry
D001713:Biphenyl Compounds; D005419:Flavonoids; D016166:Free Radical Scavengers; D010851:Picrates; D010936:Plant Extracts; D059808:Polyphenols; D012709:Serum Albumin; D002734:Chlorophyll; D002338:Carotenoids; C004931:1,1-diphenyl-2-picrylhydrazyl