Autohydrolysis of Miscanthus x giganteus for the production of xylooligosaccharides (XOS): kinetics, characterization and recovery.
The optima conditions of production and purification of xylooligosaccharides (XOS) from Miscanthus x giganteus (MxG) were investigated. Using autohydrolysis, XOS were produced at 160, 180 and 200°C at 60, 20 and 5min, respectively. XOS yield up to 13.5% (w/w) of initial biomass and 69.2% (w/w) of xylan were achieved. Results from HPAEC-PAD analysis revealed that X1-X9 sugar oligomers were produced. Higher temperature and longer reaction time resulted in lower product molecular weight. The three optimum conditions had similar degrees of polymerization XOS. Using 10% activated carbon (w/v) with ethanol/water elution recovered 47.9% (w/w) of XOS from pretreated liquid phase. The XOS could be fractionated by degree of polymerization according to ethanol concentration in the ethanol/water elution. Most of the XOS were washed out in 30% and 50% ethanol/water (v/v) fractions. Recoveries of 91.8% xylobiose, 86.9% xylotriose, 66.3% xylotetrose, 56.2% xylopentose and 48.9% xylohexaose were observed in XOS.
Agricultural and Biological Engineering, University of Illinois at Urbana-Champaign, 1304 W. Pennsylvania Avenue, Urbana, IL 61801, USA.
Ming-Hsu
MH
Chen
Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, USDA, 1815 North University Street, Peoria, IL 61604, USA.
Michael J
MJ
Bowman
Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, USDA, 1815 North University Street, Peoria, IL 61604, USA.
Bruce S
BS
Dien
Agricultural and Biological Engineering, University of Illinois at Urbana-Champaign, 1304 W. Pennsylvania Avenue, Urbana, IL 61801, USA.
Kent D
KD
Rausch
Agricultural and Biological Engineering, University of Illinois at Urbana-Champaign, 1304 W. Pennsylvania Avenue, Urbana, IL 61801, USA.
M E
ME
Tumbleson
Agricultural and Biological Engineering, University of Illinois at Urbana-Champaign, 1304 W. Pennsylvania Avenue, Urbana, IL 61801, USA. Electronic address: vsingh@illinois.edu.
Vijay
V
Singh
D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D002244:Carbon; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol; D005965:Glucuronates / Q000096:biosynthesis*; D006868:Hydrolysis; D007700:Kinetics; D009844:Oligosaccharides / Q000096:biosynthesis*; D006109:Poaceae / Q000737:chemistry / Q000378:metabolism*; D058105:Polymerization; D013696:Temperature; D013997:Time Factors
D005965:Glucuronates; D009844:Oligosaccharides; C570991:xylooligosaccharide; D000431:Ethanol; D002244:Carbon
Autohydrolysis; Degree of polymerization (DP); Miscanthus x giganteus; Pretreatment; Xylooligossaccharides (XOS)