Development of a pair of differential H/D isotope-coded derivatization reagents d(0)/d(3)-4-(1-methyl-1H-phenanthro[9,10-d]imidazol-2-yl)phenlamine and its application for determination of aldehydes in selected aquatic products by liquid chromatography-tandem mass spectrometry.
A new pair of derivatization reagents, d0-4-(1-methyl-1H-phenanthro[9,10-d]imidazol-2-yl)phenlamine (d0-MPIA) and d3-4-(1-methyl-1H-phenanthro[9,10-d]imidazol-2-yl)phenlamine (d3-MPIA) have been designed and synthesized. It was successfully used to label aliphatic aldehydes and the aldehyde derivatives were analyzed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The new isotope-coded reagents could easily label aldehydes under acidic conditions in the presence of NaCNBH3. The target derivatives exhibited intense [M+H](+) and regular product ions with electrospray ionization source in positive mode. The d0/d3-MPIA-aldehydes were monitored by the transitions of [M+H](+)→m/z 322 and [M+H](+)→m/z 165, and the obtained detection limits were in the range of 0.18-15.9 pg/mL at signal to noise ratio of 3. The global isotope internal standard technology was employed for quantification analysis with d3-MPIA-aldehyde as internal standard for corresponding d0-MPIA-aldehyde. Excellent linear responses for relative quantification were observed in the range of 1/10-10/1 with coefficients >0.998. The developed method has been applied to the quantification of aliphatic aldehydes in selected aquatic products with RSD<3.6% and recoveries >85.2%.
Key Laboratory of Life-Organic Analysis of Shandong province, Qufu Normal University, Qufu, PR China; Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University, Qufu, PR China. Electronic address: sunzhiw@126.com.
Zhiwei
Z
Sun
Key Laboratory of Life-Organic Analysis of Shandong province, Qufu Normal University, Qufu, PR China; Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University, Qufu, PR China.
Xiaoxiang
X
Wang
Key Laboratory of Life-Organic Analysis of Shandong province, Qufu Normal University, Qufu, PR China; Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University, Qufu, PR China.
Yiping
Y
Cai
Key Laboratory of Life-Organic Analysis of Shandong province, Qufu Normal University, Qufu, PR China; Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University, Qufu, PR China.
Junqing
J
Fu
Key Laboratory of Life-Organic Analysis of Shandong province, Qufu Normal University, Qufu, PR China; Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University, Qufu, PR China; Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, PR China. Electronic address: jmyou6304@163.com.
Jinmao
J
You
D000447:Aldehydes / Q000032:analysis*; D002853:Chromatography, Liquid; D007093:Imidazoles / Q000737:chemistry; D007202:Indicators and Reagents; D007554:Isotopes / Q000737:chemistry; D057230:Limit of Detection; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014867:Water / Q000032:analysis*
D000447:Aldehydes; D007093:Imidazoles; D007202:Indicators and Reagents; D007554:Isotopes; D014867:Water; C029899:imidazole
Aliphatic aldehydes; Derivatization reagent; HPLC–MS/MS; Isotope-coded