A new dried milk sampling technique and its application for progesterone detection in cows.
A new method for milk sample collection and storage, based on a dried milk sampling technique, is proposed. The method includes application of a whole milk sample to a porous membrane followed by drying. One hundred whole milk samples (dried and liquid) taken on day 21 post insemination were analysed for progesterone by ELISA and results for both dried and liquid samples were well correlated (r=0.911). Milk progesterone ELISA accuracy for pregnancy diagnosis in cows was 87%.
Faculty of Chemistry, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia. Electronic address: jvs@enz.chem.msu.ru.
Jeanne V
JV
Samsonova
Faculty of Chemistry, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia.
Alexander P
AP
Osipov
Faculty of Chemistry, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia.
Sergey E
SE
Kondakov
D000818:Animals; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005260:Female; D008892:Milk / Q000737:chemistry*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism*; D012680:Sensitivity and Specificity
D011374:Progesterone
Dried samples; ELISA; Pregnancy diagnosis in cows; Progesterone; Whole milk