Longitudinal profiling of the tissue-specific expression of genes related with insulin sensitivity in dairy cows during lactation focusing on different fat depots.
In dairy cows the milk associated energy output in early lactation exceeds the input via voluntary feed intake. To spare glucose for mammary lactose synthesis, peripheral insulin sensitivity (IS) is reduced and fat mobilization is stimulated. For these processes a link between IS and the endocrine functions of adipose tissue (AT) is likely; we thus aimed to characterise the mRNA expression from bovine AT derived proteins and receptors that are related to IS according to the literature in metabolically active tissues plus systemic IS throughout lactation. Conjugated linoleic acids (CLA) reduce milk fat thus decreasing the milk drain of energy and potentially dampening lipolysis, but may also affect IS. Subcutaneous (s.c.) AT and liver from pluriparous cows receiving either control fat or CLA supplement (100 g/day from 1 to 182 days in milk each) were biopsied covering week -3 to 36 relative to parturition. In an additional trial with primiparous cows treated analogously and slaughtered on days in milk 1, 42 or 105, samples from liver, udder, skeletal muscle and 3 visceral and 3 s.c. AT were obtained and assayed for mRNA abundance of adiponectin, its receptors, leptin, leptin receptor, PPARγ, PPARγ2, IL-6, and TNF-α. In pluriparous animals, the mRNA abundance of most of the target genes decreased after parturition in s.c. AT but increased in liver. In primiparous cows, AT depot specific differences were mostly related to retroperitoneal AT; adiponectin receptor 1 and TNF-α were affected predominantly. CLA effects in primiparous cows were largely limited to decreased PPARγ2 mRNA abundance in udder tissue. In pluriparous cows, insulin secretion was increased by CLA resulting in decreased systemic IS but without consistent changes in tissue target mRNA abundance. The temporal gene expression profiles from the adipokines and related receptors support their coactive function in adapting to the needs of lactation.
Institute of Animal Science, Physiology and Hygiene Unit, University of Bonn, Bonn, Germany.
Behnam
B
Saremi
Institute of Animal Science, Physiology and Hygiene Unit, University of Bonn, Bonn, Germany.
Sarah
S
Winand
Institute of Animal Science, Physiology and Hygiene Unit, University of Bonn, Bonn, Germany.
Paula
P
Friedrichs
Clinic for Cattle, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
Asako
A
Kinoshita
Clinic for Cattle, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
Jürgen
J
Rehage
Institute of Animal Nutrition, Friedrich-Loeffler-Institute (FLI), Federal Research Institute for Animal Health, Braunschweig, Germany.
Sven
S
Dänicke
Institute of Animal Science, Physiology and Hygiene Unit, University of Bonn, Bonn, Germany.
Susanne
S
Häussler
Department of Physiology, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
Gerhard
G
Breves
Institute of Animal Science, Physiology and Hygiene Unit, University of Bonn, Bonn, Germany.
Manfred
M
Mielenz
Institute of Animal Science, Physiology and Hygiene Unit, University of Bonn, Bonn, Germany.
Helga
H
Sauerwein
D052242:Adiponectin / Q000235:genetics / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D005223:Fats / Q000378:metabolism*; D007333:Insulin Resistance / Q000235:genetics*; D050152:Intra-Abdominal Fat / Q000378:metabolism; D007774:Lactation / Q000235:genetics* / Q000378:metabolism*; D044243:Linoleic Acids, Conjugated / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008137:Longitudinal Studies; D008892:Milk / Q000378:metabolism; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D036801:Parturition / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D054419:Receptors, Adiponectin / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
D052242:Adiponectin; D005223:Fats; D044243:Linoleic Acids, Conjugated; D047495:PPAR gamma; D012333:RNA, Messenger; D054419:Receptors, Adiponectin; D014409:Tumor Necrosis Factor-alpha