Dose-dependent response of nitrogen stable isotope ratio to proportion of legumes in diet to authenticate lamb meat produced from legume-rich diets.
This study investigated the dose-dependent response in lamb meat of stable nitrogen isotope ratio to the dietary proportion of legumes, and the ability of the nitrogen isotope signature of the meat to authenticate meat produced from legume-rich diets. Four groups of nine male Romane lambs grazing a cocksfoot pasture were supplemented with different levels of fresh alfalfa forage to obtain four dietary proportions of alfalfa (0%, 25%, 50% and 75%) for 98 days on average before slaughter (groups L0, L25, L50 and L75). We measured the stable nitrogen isotope ratio in the forages and in the longissimus thoracis muscle. The δ(15)N value of the meat decreased linearly with the dietary proportion of alfalfa. The distribution of the δ(15)N values of the meat discriminated all the L0 lambs from the L75 lambs, and gave a correct classification score of 85.3% comparing lambs that ate alfalfa with those that did not.
Institut National de la Recherche Agronomique (INRA), UMR1213 Herbivores, F-63122 St-Genès-Champanelle, France; Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves 7712, CEP 91501-970, Porto Alegre, RS, Brazil.
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Devincenzi
Institut National de la Recherche Agronomique (INRA), UMR0614 Fractionnement des Agro Ressources et Environnement, 2 esplanade Roland-Garros, BP 224, F-51686 Reims Cedex 2, France.
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Delfosse
Institut National de la Recherche Agronomique (INRA), UMR1213 Herbivores, F-63122 St-Genès-Champanelle, France.
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Andueza
Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves 7712, CEP 91501-970, Porto Alegre, RS, Brazil.
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Nabinger
Institut National de la Recherche Agronomique (INRA), UMR1213 Herbivores, F-63122 St-Genès-Champanelle, France. Electronic address: sophie.prache@clermont.inra.fr.
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Prache
D000821:Animal Feed / Q000032:analysis*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D016002:Discriminant Analysis; D007887:Fabaceae / Q000737:chemistry* / Q000378:metabolism; D008460:Meat / Q000032:analysis*; D009587:Nitrogen Isotopes / Q000032:analysis*; D011786:Quality Control; D012756:Sheep / Q000378:metabolism*
D009587:Nitrogen Isotopes
Authentication; Lamb meat; Legumes; Nitrogen stable isotope ratio; δ(15)N