Antibacterial and antioxidant activities of ursolic acid and derivatives.
Ursolic acid, an important bioactive compound, was isolated from ethanol extract of aerial parts of Sambucus australis. In order to develop bioactive ursolic acid derivatives, two semi-synthetic compounds were obtained through modification at C-3. The antibacterial activity of the ursolic acid and its derivatives was investigated. The microdilution method was used for determination of the minimal inhibitory concentration (MIC), against twelve bacterial strains. The influence of ursolic acid and its derivatives on the susceptibility of some bacterial pathogens to the aminoglycosides antibiotics neomycin, amikacin, kanamycin and gentamicin was evaluated. The most representative synergistic effect was observed by 3β-formyloxy-urs-12-en-28-oic acid at the concentration of 64 μg/mL in combination with kanamycin against Escherichia coli (27), a multidrug-resistant clinical isolate from sputum, with reduction of MIC value from 128 μg/mL to 8 μg/mL. Ursolic acid and its derivatives were examined for their radical scavenger activity using the DPPH assay, and showed significant activity.
Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Fortaleza-CE 60451-970, Brazil. georgina.quimica@gmail.com.
Patrícia G G
PG
do Nascimento
Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Fortaleza-CE 60451-970, Brazil. tlglemos@yahoo.com.br.
Telma L G
TL
Lemos
Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Fortaleza-CE 60451-970, Brazil. aylamarcia@yahoo.com.br.
Ayla M C
AM
Bizerra
Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Fortaleza-CE 60451-970, Brazil.
Ângela M C
ÂM
Arriaga
Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Fortaleza-CE 60451-970, Brazil. dafufc@yahoo.com.br.
Daniele A
DA
Ferreira
Departamento de Farmácia, Universidade Federal do Ceará, Fortaleza-CE 60430-370, Brazil. gil@ufc.br.
Gilvandete M P
GM
Santiago
Laboratório de Ciências Químicas (LCQUI)-CCT-UENF/PPGQO-DEQUIM-UFRRJ, Campos dos Goytacazes-RJ 28013-602, Brazil. braz@uenf.br.
Raimundo
R
Braz-Filho
Laboratório de Pesquisa de Produtos Naturais, Universidade Regional do Cariri, Crato-CE 63105-000, Brazil. galberto.martins@gmail.com.
José Galberto M
JG
Costa
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D004357:Drug Synergism; D020128:Inhibitory Concentration 50; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D014315:Triterpenes / Q000737:chemistry* / Q000494:pharmacology*; D000097245:Ursolic Acid
D000900:Anti-Bacterial Agents; D000975:Antioxidants; D014315:Triterpenes; D000097245:Ursolic Acid