Canola Cake as a Potential Substrate for Proteolytic Enzymes Production by a Selected Strain of Aspergillus oryzae: Selection of Process Conditions and Product Characterization.
Oil cakes have excellent nutritional value and offer considerable potential for use in biotechnological processes that employ solid-state fermentation (SSF) for the production of high value products. This work evaluates the feasibility of using canola cake as a substrate for protease production by a selected strain of Aspergillus oryzae cultivated under SSF. The influences of the following process parameters were considered: initial substrate moisture content, incubation temperature, inoculum size, and pH of the buffer used for protease extraction and activity analysis. Maximum protease activity was obtained after cultivating Aspergillus oryzae CCBP 001 at 20°C, using an inoculum size of 10(7) spores/g in canola cake medium moistened with 40 mL of water to 100 g of cake. Cultivation and extraction under selected conditions increased protease activity 5.8-fold, compared to the initial conditions. Zymogram analysis of the enzymatic extract showed that the protease molecular weights varied between 31 and 200 kDa. The concentrated protease extract induced clotting of casein in 5 min. The results demonstrate the potential application of canola cake for protease production under SSF and contribute to the technological advances needed to increase the efficiency of processes designed to add value to agroindustrial wastes.
Department of Food Engineering, Federal University of Maranhão, Rua Urbano Santos S/N, 65900-410 Imperatriz, MA, Brazil ; Department of Chemical Engineering, Federal University of Ceará, Avenida da Universidade 2853, 60020-181 Fortaleza, CE, Brazil.
Adriana C
AC
Freitas
Embrapa Tropical Agroindustry, Rua Doutora Sara Mesquita 2270, 60511-110 Fortaleza, CE, Brazil.
Ruann J S
RJ
Castro
Department of Food Engineering, Federal University of Maranhão, Rua Urbano Santos S/N, 65900-410 Imperatriz, MA, Brazil.
Maria A
MA
Fontenele
Embrapa Goats and Sheep, Estrada Sobral/Groaíras km 04, 62010-970 Sobral, CE, Brazil.
Antonio S
AS
Egito
Embrapa Instrumentation, Rua XV de Novembro 1452, 13560-970 São Carlos, SP, Brazil.
Cristiane S
CS
Farinas
Embrapa Tropical Agroindustry, Rua Doutora Sara Mesquita 2270, 60511-110 Fortaleza, CE, Brazil.
Gustavo A S
GA
Pinto