In situ generation of cell-laden porous MMP-sensitive PEGDA hydrogels by gelatin leaching.
Proteolytically degradable poly(ethylene) glycol (PEG) hydrogels have been investigated as tissue engineering scaffolds; however, cell invasion and tissue regeneration are limited by the rate of cell-mediated degradation due to the small mesh size of the resultant crosslinked network. Gelatin leaching is combined with photopolymerization to form porous matrix-metalloproteinase (MMP)-sensitive PEG scaffolds under cytocompatible conditions in the presence of cells. Gelatin leaching allows control over pore size and porosity through selectivity of gelatin bead particle size and porogen loading, respectively. Increases in porogen loading lead to increased porosity, decreased compressive modulus and degradation time, and enhanced proliferation of encapsulated vascular smooth muscle cells.
Department of Biomedical Engineering, Illinois Institute of Technology, 3255 South Dearborn Street, Wishnick Hall Room 223, Chicago, IL, 60616, USA.
Sonja
S
Sokic
Megan
M
Christenson
Jeffery
J
Larson
Georgia
G
Papavasiliou
D000179:Acrylates / Q000737:chemistry*; D000704:Analysis of Variance; D049109:Cell Proliferation; D005453:Fluorescence; D005780:Gelatin / Q000737:chemistry; D020100:Hydrogels / Q000138:chemical synthesis* / Q000737:chemistry*; D007700:Kinetics; D020782:Matrix Metalloproteinases / Q000737:chemistry; D008858:Microscopy, Phase-Contrast; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000502:physiology; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D016062:Porosity; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
D000179:Acrylates; D020100:Hydrogels; D011092:Polyethylene Glycols; D005780:Gelatin; D020782:Matrix Metalloproteinases
PEGDA; degradation; hydrogels; matrix metalloproteinases; porosity