[Effects of fluoride on oxidative stress and apoptosis in primary culture of rat sertoli cells].
OBJECTIVE
To study effects of fluoride on oxidative stress and apoptosis in rat sertoli cells.


METHODS
Intracellular reactive oxygen species (ROS) , malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, mitochondrial membrane potential and percentage of apoptosis were measured after the rat sertoli cells were incubated with 0, 6, 12 and 24 microg/ml sodium fluorides or 24 hours in vitro.


RESULTS
Fluoride decreased the cell activity significantly (P < 0.01). The results suggested that exposure to fluoride significantly increased the level of ROS and MDA content (P < 0.01), fluoride also decreased SOD activity significantly (P < 0.05 or P < 0.01). In addition, with the NaF dose increased, there is a significantly decreasing in mitochondrial membrane potential and a significantly increasing in early apoptosis rate (P < 0.01).


CONCLUSIONS
Fluoride can induce excessive oxidative stress and increased apoptosis rate in rat sertoli cells.
Department of Environmental Health, College of Public Health, Zhengzhou University, Zhengzhou 450001, China. tangshuiyy@163.com
Yang
Y
Yang
Department of Environmental Health, College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
Hui
H
Huang
Department of Environmental Health, College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
Wei
W
Yuan
Department of Environmental Health, College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
Demin
D
Feng
Department of Environmental Health, College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
Zhijin
Z
Wang
Department of Environmental Health, College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
Xuemin
X
Cheng
Department of Environmental Health, College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
Yue
Y
Ba
Department of Environmental Health, College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
Liuxin
L
Cui
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D061251:Primary Cell Culture; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D012708:Sertoli Cells / Q000166:cytology* / Q000378:metabolism; D012969:Sodium Fluoride / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism
D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D012969:Sodium Fluoride; D013482:Superoxide Dismutase