Positron emission tomography imaging of cell death with [(18)F]FPDuramycin.
The noninvasive imaging of cell death, including apoptosis and necrosis, is an important tool for the assessment of degenerative diseases and in the monitoring of tumor treatments. Duramycin is a peptide of 19-amino acids. It binds specifically to phosphatidylethanolamine a novel molecular target for cell death. N-(2-(18)F-Fluoropropionyl)duramycin ([(18)F]FPDuramycin) was prepared as a novel positron emission tomography (PET) tracer from the reaction of duramycin with 4-nitrophenyl 2-[(18)F]fluoropropionate ([(18)F]NFP). Compared with control cells (viable tumor cells), the in vitro binding of [(18)F]FPDuramycin with apoptotic cells induced by anti-Fas antibody resulted in a doubling increase, while the binding of [(18)F]FPDuramycin with necrotic cells induced by three freeze and thaw cycles resulted in a threefold increase. Biodistribution study in mice exhibited its rapid blood and renal clearance and predominant accumulation in liver and spleen over 120 min postinjection. Small-animal PET/CT imaging with [(18)F]FPDuramycin proved to be a successful way to visualize in vivo therapeutic-induced tumor cell death. In summary, [(18)F]FPDuramycin seems to be a potential PET probe candidate for noninvasive visualization of in vivo cell death sites induced by chemotherapy in tumors.
School of Pharmaceutical Science and Technology, Tianjin University, 300072, Tianjin, China, yaoshaobo008@163.com.
Shaobo
S
Yao
Kongzhen
K
Hu
Ganghua
G
Tang
Xiang
X
Liang
Kan
K
Du
Dahong
D
Nie
Shende
S
Jiang
Linquan
L
Zang
D000818:Animals; D017209:Apoptosis; D001430:Bacteriocins / Q000138:chemical synthesis / Q000737:chemistry* / Q000493:pharmacokinetics; D016923:Cell Death; D005260:Female; D006801:Humans; D019169:Jurkat Cells; D051379:Mice; D008819:Mice, Nude; D057054:Molecular Imaging; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000493:pharmacokinetics; D049268:Positron-Emission Tomography; D019275:Radiopharmaceuticals / Q000138:chemical synthesis / Q000737:chemistry* / Q000493:pharmacokinetics; D014018:Tissue Distribution
D001430:Bacteriocins; C000592888:N-(2-fluoropropionyl)duramycin; D010455:Peptides; D019275:Radiopharmaceuticals