Production of bioactive cyathane diterpenes by a bird's nest fungus Cyathus gansuensis growing on cooked rice.
Cyathane diterpenes are important bioactive substances produced by some edible and medicinal fungi. Seven new cyathane type diterpenes, named as cyathins J-P (1-7), together with two known diterpenes (8 and 9), were isolated from the solid culture of the bird's nest fungus Cyathus gansuensis growing on cooked rice. The structures of the new secondary metabolites were elucidated by NMR experiments. Bioactivity screening indicated that compounds 1, 2, 4, and 8 showed moderate inhibitory activity against NO production in lipopolysaccharide-activated macrophages with an IC50 value of 42, 78, 80, and 16 μM, respectively. The fungus C. gansuensis is a promising source of bioactive secondary metabolites and has application potential in preparing healthy food.
School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, No. 103 Wenhua Road, ShenHe District, Shenyang 110016, PR China; State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No. 8 Beiertiao, Zhongguancun, Haidian District, Beijing 100190, PR China.
Botao
B
Wang
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No. 8 Beiertiao, Zhongguancun, Haidian District, Beijing 100190, PR China.
Junjie
J
Han
School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, No. 103 Wenhua Road, ShenHe District, Shenyang 110016, PR China. Electronic address: shxuwei8720@163.com.
Wei
W
Xu
College of Life Science, Southwest Forestry University, Kunming 650224, PR China.
Yuhui
Y
Chen
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No. 8 Beiertiao, Zhongguancun, Haidian District, Beijing 100190, PR China. Electronic address: liuhw@im.ac.cn.
Hongwei
H
Liu
D000818:Animals; D002460:Cell Line; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D055365:Cyathus / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D004224:Diterpenes / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D005285:Fermentation; D008264:Macrophages / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D015394:Molecular Structure; D012275:Oryza / Q000737:chemistry / Q000382:microbiology*; D064210:Secondary Metabolism
D003470:Culture Media; D004224:Diterpenes; C497675:cyathane
Cyathane diterpenes; Cyathus gansuensis; NO inhibition; Structure elucidation