Potential halophilic cellulases for in situ enzymatic saccharification of ionic liquids pretreated lignocelluloses.
Ionic liquids (ILs) have been used as an alternative green solvent for lignocelluloses pretreatment. However, being a salt, ILs exhibit an inhibitory effect on cellulases activity, thus making the subsequent saccharification inefficient. The aim of the present study is to produce salt-tolerant cellulases, with the rationale that the enzyme also tolerant to the presence of ILs. The enzyme was produced from a locally isolated halophilic strain and was characterized and assessed for its tolerance to different types of ionic liquids. The results showed that halophilic cellulases produced from Aspergillus terreus UniMAP AA-6 exhibited higher tolerance to ILs and enhanced thermo stability in the presence of high saline conditions.
School of Bioprocess Engineering, Universiti Malaysia Perlis, Kompleks Pusat Pengajian Jejawi 3, 02600 Arau, Perlis, Malaysia.
Ahmad Anas Nagoor
AA
Gunny
School of Bioprocess Engineering, Universiti Malaysia Perlis, Kompleks Pusat Pengajian Jejawi 3, 02600 Arau, Perlis, Malaysia. Electronic address: dachyar@unimap.edu.my.
Dachyar
D
Arbain
School of Bioprocess Engineering, Universiti Malaysia Perlis, Kompleks Pusat Pengajian Jejawi 3, 02600 Arau, Perlis, Malaysia.
Rizo
R
Edwin Gumba
Agrotechnology and Biosciences Division, Malaysian Nuclear Agency (Nuclear Malaysia), Ministry of Science, Technology and Innovation, Bangi, 43000 Kajang, Selangor, Malaysia.
Bor Chyan
BC
Jong
Bioenvironmental Engineering Research Center (BERC), Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, 50728 Gombak, Kuala Lumpur, Malaysia.
Parveen
P
Jamal
D001230:Aspergillus / Q000201:enzymology* / Q000235:genetics; D002480:Cellulase / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D006868:Hydrolysis; D052578:Ionic Liquids / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D054712:Salinity; D013696:Temperature
D017931:DNA Primers; D052578:Ionic Liquids; C036909:lignocellulose; D008031:Lignin; D002480:Cellulase
Cellulases; Halophiles; Ionic liquids; Lignocelluloses; Saccharification