Association between the g.14461A>G genetic polymorphism of the TLR4 gene and type 2 diabetes mellitus risk in a Chinese population.
OBJECTIVE
Toll-like receptor 4 (TLR4) is an important candidate gene for mediating the susceptibility to type 2 diabetes mellitus (T2DM). The purpose of this study was to investigate the association between the TLR4 gene polymorphisms and T2DM susceptibility.


METHODS
A total of 671 T2DM patients and 677 healthy controls were recruited in this study. The created restriction site-polymerase chain reaction and DNA sequencing methods have been used to analyze the TLR4 gene polymorphisms.


RESULTS
One novel genetic polymorphism (g.14461A>G) was found. Our data indicated that the g.14461A>G genetic polymorphism was significantly associated with the increased susceptibility to T2DM in a homozygote comparison (GG vs. AA: odds ratio [OR]=2.09, 95% confidence interval [CI] 1.44-3.04, p<0.001), dominant model (GG/AG vs. AA: OR=1.27, 95% CI 1.03-1.57, p=0.028), recessive model (GG vs.
OR=1.98, 95% CI 1.39-2.83, p<0.001), and allele contrast (G vs. A: OR=1.33, 95% CI 1.13-1.57, p=0.001). The allele-G might be the risk allele for enhancing the susceptibility to T2DM.


CONCLUSIONS
These preliminary findings suggest that the g.14461A>G genetic polymorphism of the TLR4 gene is potentially related to the susceptibility to T2DM in the studied population.
1 Department of Endocrinology, Tongji Hospital, Tongji University School of Medicine , Shanghai, People's Republic of China .
Tao
T
Lei
Wenjun
W
Tang
Yanqin
Y
Xiong
Yujuan
Y
Zhai
Xianghua
X
Sun
Keqin
K
Zhang
D000328:Adult; D000368:Aged; D000483:Alleles; D001483:Base Sequence; D016022:Case-Control Studies; D002681:China; D017931:DNA Primers; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006720:Homozygote; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D051197:Toll-Like Receptor 4 / Q000235:genetics*
D017931:DNA Primers; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4