Sister chromatid exchange, (SCE), High-Frequency Cells (HFCs) and SCE distribution patterns in peripheral blood lymphocytes of Spanish adult smokers compared to non-smokers.
According to the International Agency for Research on Cancer, smoking tobacco is a major cause of cancer in humans. It causes about half of all male cancer deaths and an ever increasing number of cancer deaths in females. The aim of this study was to establish whether cigarette smoking increases sister chromatid exchanges (SCEs) in peripheral blood lymphocytes in two Spanish population groups; light and heavy smokers. The mean number of High-Frequency Cells (HFCs) was determined and, the SCE distribution pattern among the chromosomes was analysed represented by a ratio described below. A local sample of 101 adult smokers (n=48) and non-smokers (n=53), aged from 18 to 49 years, was studied using SCE levels in peripheral lymphocytes. Heavy smoking (≥ 10 cigarettes per day) increased significantly the SCE frequency and the HFC parameters. Neither age nor sex significantly influenced the frequencies in the groups studied.
Department de Medicina Preventiva i Salut Pública, Facultat de Farmàcia, Universitat de Valencia, Burjassot, Spain.
Natividad
N
Sebastià
Unidad de Bioestadística IIS La Fe, Instituto de Investigación Sanitaria La Fe, Valencia, Spain.
David
D
Hervás
Servicio de Protección Radiológica, Hospital Universitario Politécnico La Fe, Valencia, Spain.
Miguel
M
Almonacid
Servicio de Protección Radiológica, Hospital Universitario Politécnico La Fe, Valencia, Spain.
Juan Ignacio
JI
Villaescusa
Department de Medicina Preventiva i Salut Pública, Facultat de Farmàcia, Universitat de Valencia, Burjassot, Spain. Electronic address: jose.soriano@uv.es.
José Miguel
JM
Soriano
Servicio de Protección Radiológica, Hospital Universitario Politécnico La Fe, Valencia, Spain.
Vicenta
V
Sahuquillo
Servicio de Salud Laboral, Dirección General de Salud Pública, Consellería de Sanitat, Generalitat Valenciana, Spain.
Valentín
V
Esteban
Institut de Radioprotection et SûretéNucléaire (IRSN), PRP-HOM, SRBE, LDB.BP017, 92262 Fontenay-aux-Roses, France.
Joan Francesc
JF
Barquinero
Departamento de Ingeniería Química y Nuclear, Univeristat Politécnica de Valencia, Camino de Vera s/n, Spain.
Gumersindo
G
Verdú
Servicio de Hematología, Hospital Universitario y Politécnico La Fe, Valencia, Spain.
José
J
Cervera
Servicio de Hematología, Hospital Universitario y Politécnico La Fe, Valencia, Spain.
Esperanza
E
Such
Servicio de Protección Radiológica, Hospital Universitario Politécnico La Fe, Valencia, Spain.
Alegría
A
Montoro
D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure*; D008297:Male; D008875:Middle Aged; D012854:Sister Chromatid Exchange; D012907:Smoking / Q000235:genetics*; D013030:Spain; D055815:Young Adult
Biomarker; Cytogenetic assessment; SCEs; Tobacco