Evaluation of crocin and curcumin affinity on mushroom tyrosinase using surface plasmon resonance.
Tyrosinase inhibitors have potential applications in the cosmetics and food industries for preventing browning reactions and also as therapeutic drugs for neurodegenerative diseases such as Parkinson's. In this article, crocin and curcumin were evaluated as mushroom tyrosinase inhibitors. Results showed that, both compounds strongly inhibited the diphenolase activity than monophenolase. The IC50 values for diphenolase activity were estimated to be 0.11 mM and 0.18 mM for crocin and curcumin respectively. The binding kinetics of crocin and curcumin was studied with mushroom tyrosinase using surface plasmon resonance (SPR). Tyrosinase was immobilized on the gold surface of a Biacore sensor chip through amine coupling. Binding of inhibitors was analyzed by SPR without the need to further modify the surface or the use of other reagents. The binding constant KD (M) for mushroom tyrosinase obtained was 1.21×10(-4) M for crocin and 1.64×10(-4) M for curcumin, while showing a higher affinity for L-DOPA 1.95×10(-8) M, a substrate for tyrosinase (positive control). The study reveals the SPR sensor's ability to detect binding of the inhibitors.
Department of Biotechnology, Shivaji University, Kolhapur 416004, India.
Sushama
S
Patil
GE Healthcare Life Sciences, John F Welch Technology Centre, EPIP, Phase 2, Whitefield Road, Bangalore 560048, India.
Sistla
S
Srinivas
Department of Biotechnology, Shivaji University, Kolhapur 416004, India; Department of Biochemistry, Shivaji University, Kolhapur 416004, India. Electronic address: jpjbiochem@gmail.com.
Jyoti
J
Jadhav
D000363:Agaricales / Q000201:enzymology*; D002338:Carotenoids / Q000378:metabolism* / Q000494:pharmacology; D003474:Curcumin / Q000378:metabolism* / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000378:metabolism* / Q000494:pharmacology; D004800:Enzymes, Immobilized / Q000037:antagonists & inhibitors / Q000378:metabolism; D007980:Levodopa / Q000378:metabolism / Q000494:pharmacology; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011485:Protein Binding; D020349:Surface Plasmon Resonance
D004791:Enzyme Inhibitors; D004800:Enzymes, Immobilized; D002338:Carotenoids; D007980:Levodopa; C029036:crocin; D014442:Monophenol Monooxygenase; D003474:Curcumin
Binding kinetics; Mushroom tyrosinase; Surface plasmon resonance