Microbial transformation of resibufogenin by Curvularia lunata AS 3.4381.
In this paper, the microbial transformation of resibufogenin by Curvularia lunata AS 3.4381 was investigated, and four transformed products were isolated and characterized as 3-epi-resibufogenin (2), 12α-hydroxy-3-epi-resibufogenin (3), 12-oxo-16β-hydroxy-3-epi-resibufogenin (4), and 12β,15-epoxy-3-epi-bufalin-14,15-ene (5). Among them, 4 and 5 are new compounds, and isomerization, hydroxylation, and oxidation reactions in microbial transformation process were observed. Additionally, the cytotoxicities of transformed products (2-5) were also investigated.
a College of Bioengineering, Beijing Polytechnic , Beijing , 100029 , China.
Xiu-Lan
XL
Xin
Jiang-Hao
JH
Sun
Xiao-Bo
XB
Wang
Rong-Gang
RG
Xi
Gang
G
Wang
Rong
R
Lan
Dong-Hai
DH
Su
Hua
H
Li
Xiao-Kui
XK
Huo
Chao
C
Wang
D001203:Ascomycota / Q000737:chemistry / Q000378:metabolism*; D002018:Bufanolides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006367:HeLa Cells; D006801:Humans; D006900:Hydroxylation; D007536:Isomerism; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular
C587296:12-oxo-16beta-hydroxy-3-epi-resibufogenin; C587297:12beta,15-epoxy-3-epi-bufalin-14,15-ene; D002018:Bufanolides; C005734:bufogenin; C022777:bufalin