Serum peptide mapping in gastric precancerous lesion and cancer.
OBJECTIVE
To construct and verify a diagnostic model using proteomic analysis of serum samples for identifying gastric precancerous lesions and gastric cancer (GC).


METHODS
The serum samples from 25 patients with gastric precancerous lesions (chronic atrophic gastritis with mild to moderate dysplasia), 25 GC patients and 25 healthy controls were analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Spectral peaks with significant difference among the groups were identified and used as a diagnostic model for detecting gastric precancerous lesions and GC. The serum peptide map model was validated using an independent sample set including 15 healthy volunteers, 15 precancerous and 15 GC patients.


RESULTS
The spectral peaks for the peptides with mass-to-charge (m/z) values of 1741 and 4210 were the most significantly different among the three groups. The sensitivity of this diagnostic model for detecting healthy controls, patients with gastric precancerous lesions and patients with GC was 80.0% (12/15), 66.7% (10/15) and 66.7% (10/15) respectively, while the specificity was 66.7% (20/30), 73.3% (22/30) and 73.3% (22/30), respectively.


CONCLUSIONS
Our diagnostic model is useful for diagnosing gastric precancerous lesions and GC.
Department of Digestive Diseases, Beijing Friendship Hospital, Capital Medical University, Beijing Key Laboratory for Precancerous Lesion of Digestive Diseases, Beijing Digestive Diseases Center.
Peng
P
Li
Dan
D
Ma
Sheng Tao
ST
Zhu
Xu Dong
XD
Tang
Shu Tian
ST
Zhang
D000293:Adolescent; D000328:Adult; D000368:Aged; D001798:Blood Proteins / Q000032:analysis*; D005260:Female; D005757:Gastritis, Atrophic / Q000097:blood / Q000175:diagnosis; D006801:Humans; D008297:Male; D008875:Middle Aged; D010449:Peptide Mapping / Q000379:methods* / Q000592:standards; D011230:Precancerous Conditions / Q000097:blood / Q000175:diagnosis*; D040901:Proteomics / Q000379:methods* / Q000592:standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods / Q000592:standards; D013274:Stomach Neoplasms / Q000097:blood / Q000175:diagnosis*; D055815:Young Adult
D001798:Blood Proteins
atrophic gastritis; diagnosis; dysplasia; matrix-assisted laser desorption/ionization mass spectrometry; stomach neoplasms