Roles of GST-π and polβ genes in chemoresistance of esophageal carcinoma cells.
The main aim of this study was to investigate the roles of GST-π and polβ genes in the chemoresistance of esophageal carcinoma cells. Eukaryotic expression vectors containing each gene were constructed and transfected into EC9706 cells, and the biological effects of the two genes assessed based on a resistance index. We additionally investigated the in vitro and in vivo anti-resistance effects of GST-π and polβ genes using recombinant lentiviruses carrying siRNAs against the two genes. Our results showed that upregulation of GST-π and polβ genes suppresses chemosensitivity of esophageal carcinoma cells to cisplatin, while downregulation of these two genes with RNAi technology reverses this chemoresistance. Multi-site injection of recombinant lentivirus targeting the GST-π gene into transplanted cDDP tumors effectively reversed their chemoresistant phenotype. However, the same treatment against the polβ gene did not lead to significant efficacy against chemoresistance.
College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China E-mail : liminzzu@gmail.com.
Yue
Y
Tang
Xiao-Yan
XY
Xuan
Min
M
Li
Zi-Ming
ZM
Dong
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D015153:Blotting, Western; D049109:Cell Proliferation; D002945:Cisplatin / Q000494:pharmacology*; D019951:DNA Polymerase beta / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D051549:Glutathione S-Transferase pi / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
D000970:Antineoplastic Agents; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D051549:Glutathione S-Transferase pi; D019951:DNA Polymerase beta; D002945:Cisplatin