Effects of glass bead size, vortexing speed and duration on Eimeria acervulina oocyst excystation.
Improved methods for efficient excystation of Eimeria should be developed and standardized for future Eimeria-related studies. Here, the effects of different glass bead sizes (0.5, 1, 2, and 2.5 mm), and various vortex speeds (1000, 2000, and 3000 rpm) and durations (30 s, 1, 3, and 5 min) have been examined for Eimeria (E.) acervulina oocyst excystation. At 3000 rpm, all glass beads, regardless of size, efficiently ruptured E. acervulina oocysts at 5 min. At 2000 and 3000 rpm, all four glass bead sizes increasingly ruptured oocysts in a time-dependent manner. In contrast, at 1000 rpm the excystation efficiency was not related with the glass bead size or with vortexing duration. It appeared that the 1mm glass beads are most efficient for E. acervulina DNA extraction at a 3000 rpm vortexing speed for 3 and 5 min. The 2 mm glass beads delicately released the highest number of intact sporocysts at 2000 rpm for 3 min. Therefore, our data can provide valuable information for the efficient mechanical excystation of E. acervulina.
Biosafety Research Institute and Laboratory of Pathology, College of Veterinary Medicine (BK21 Plus Program), Chonbuk National University, Jeonju, Republic of Korea.
Jang-Ock
JO
Cha
Biosafety Research Institute and Laboratory of Pathology, College of Veterinary Medicine (BK21 Plus Program), Chonbuk National University, Jeonju, Republic of Korea.
Abul Fatah Shah Muhammad
AF
Talha
Biosafety Research Institute and Laboratory of Pathology, College of Veterinary Medicine (BK21 Plus Program), Chonbuk National University, Jeonju, Republic of Korea.
Chae Woong
CW
Lim
Biosafety Research Institute and Laboratory of Pathology, College of Veterinary Medicine (BK21 Plus Program), Chonbuk National University, Jeonju, Republic of Korea. Electronic address: bskims@jbnu.ac.kr.
Bumseok
B
Kim
D000818:Animals; D002645:Chickens / Q000469:parasitology; D003048:Coccidiosis / Q000469:parasitology / Q000662:veterinary; D021901:DNA, Intergenic / Q000737:chemistry / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D004539:Eimeria / Q000235:genetics / Q000502:physiology*; D005898:Glass; D008863:Microspheres; D033661:Oocysts / Q000502:physiology; D010316:Particle Size; D016133:Polymerase Chain Reaction; D011201:Poultry Diseases / Q000469:parasitology; D012044:Regression Analysis; D012399:Rotation; D013997:Time Factors
D021901:DNA, Intergenic; D016054:DNA, Protozoan
Eimeria acervulina; Excystation; Glass bead