Rat intestinal sucrase inhibition of constituents from the roots of Rosa rugosa Thunb.
A new octanordammarane triterpene, 3β,15α-dihydroxymansumbinol (1) and a novel A-ring contracted oleanane triterpenoid, 2-formyl-(A)1-19α-hydroxy-1-norolean-2,12-dien-28-oic acid (2) were isolated from the roots extract of Rosa rugosa along with fifteen known compounds (3-17). Their structures were elucidated by extensive spectroscopic analysis, including 1D and 2D NMR, and FTICRMS. The MeOH extract, as well as CH2Cl2 and EtOAc fractions at a concentration of 0.5mg/mL showed potent sucrase inhibitory activity, with inhibition percentage values of 84.67±5.37%, 87.50±2.78%, and 81.91±2.90%, respectively. In addition, compounds 7-13 (1.0 mM) showed potent sucrase inhibitory activity (61.88±3.19% to 84.70±3.07% inhibition), which was comparable to that of the positive control, acarbose, with an inhibition percentage value of 50.96±2.97%. Compounds 1, 2, 4, and 14-17 showed moderate and/or weak inhibitory activities at the same concentration. The α-glucosidase inhibitory activities of the extracts and purified compounds may provide a novel opportunity to develop a new class of antidiabetic agents.
College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea; Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghiado, Caugiay, Hanoi, Viet Nam.
Nguyen Phuong
NP
Thao
College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea; Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghiado, Caugiay, Hanoi, Viet Nam.
Bui Thi Thuy
BT
Luyen
Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghiado, Caugiay, Hanoi, Viet Nam.
Bui Huu
BH
Tai
College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea.
Seo Young
SY
Yang
Department of Food and Nutrition, Hannam University, Daejeon 305-811, Republic of Korea.
Sung Hoo
SH
Jo
Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghiado, Caugiay, Hanoi, Viet Nam.
Nguyen Xuan
NX
Cuong
Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghiado, Caugiay, Hanoi, Viet Nam.
Nguyen Hoai
NH
Nam
Department of Food and Nutrition, Hannam University, Daejeon 305-811, Republic of Korea.
Young In
YI
Kwon
Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghiado, Caugiay, Hanoi, Viet Nam.
Chau Van
CV
Minh
College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea. Electronic address: yhk@cnu.ac.kr.
Young Ho
YH
Kim
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007421:Intestine, Small / Q000201:enzymology*; D008968:Molecular Conformation; D018517:Plant Roots / Q000737:chemistry*; D051381:Rats; D027826:Rosa / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013393:Sucrase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
C000592176:2-formyl-(A)1-hydroxy-1-norolean-2,12-dien-28-oic acid; C000592177:3beta,15alpha-dihydroxymansumbinol; D004791:Enzyme Inhibitors; D014315:Triterpenes; D013393:Sucrase
A-ring contracted oleanane triterpenoid; Rosa rugosa; Rosaceae; Rosanol A; Rusaic acid B; α-Glucosidase inhibition