Effect of follicular fluid and platelet-activating factor on lactate dehydrogenase C expression in human asthenozoospermic samples.
BACKGROUND
Application of follicular fluid (FF) and platelet-activating factor (PAF) in artificial insemination improves sperm motility. Lactate dehydrogenase C (LDH-C) is a key enzyme for sperm motility. In this study, the effects of FF and PAF on the sperm motility index and LDH-C expression were investigated. Moreover, LDH-C expression was compared between asthenozoospermic and normozoospermic samples.


METHODS
The expression of LDH-C was examined by quantitative real-time polymerase chain reaction (q-RT PCR) and western blotting after it was treated with optimized concentrations of FF and PAF in twenty asthenozoospermic samples. Also, LDH-C expression was evaluated in five normozoospermic samples.


RESULTS
Samples with 75% FF and 100 nM of PAF had an increase in their percentages of progressive and slowly motile sperms and a decrease in their percentages of non-progressive and non-motile sperms. Moreover, LDH-C mRNA transcripts were not changed following PAF and FF treatment, and LDH-C protein was detected in highly progressive motile specimens treated with FF in the asthenozoospermic samples. Furthermore, LDH-C expression was more detectable in the normal sperms.


CONCLUSIONS
Our results indicated that PAF had more beneficial effects than FF on sperm motility in the asthenozoospermic samples (P=0.0001), although the LDH-C expressions of the sperms were not changed significantly in both groups. We found no association between LDH-C expression and sperm motility after FF and PAF actions. This finding, however, requires further investigation. The fact that LDH-C protein was detected in the normozoospermic, but not asthenozoospermic, samples could be cited as a reason for the infertility in these patients.
Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;
Tahereh
T
Esmaeilpour
Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;
Mohmmad Reza
MR
Zarei
Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;
Soghra
S
Bahmanpour
Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;
Elham
E
Aliabadi
Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Ahmad
A
Hosseini
Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Mansooreh
M
Jaberipour
Asthenozoospermia; Follicular fluid; LDH-C; Platelet-activating factor; Sperm motility