Blood ALDH1 and GST activity in diabetes type 2 and its correlation with glycated hemoglobin.
There is increasing evidence that oxidative stress (OS) plays a major role in the pathogenesis of diabetes mellitus (DM) and the development of its complications. As one of the consequences of OS is increased lipid peroxidation (LP), the aim of our studies was to check, how the activity of 2 enzymes involved in the detoxification of aldehydes formed during LP, glutathione S-transferase (GST) and aldehyde dehydrogenase 1 (ALDH 1) is changed in patients suffering from DM.GST and ALDH1A1 activities were determined in whole blood samples of DM type 2 patients (n=64) and healthy controls (n=60) using spectrophotometer (for GST activity) and fluorometer (for ALDH1 activity) and they were found to be significantly increased in diabetics when compared with healthy control (p<0.05). Intriguingly, grouping the DM patients on the basis of the glucose level and HbA1c revealed unusually low ALDH activity in the group of patients (n=16) with a relatively high level of these 2 parameters.The increase of ALDH1A1 and GST activity in DM seems to be associated with the severity of the disease and might be a compensatory effect against oxidative stress. Surprisingly low ALDH activity in DM patients with relatively high glucose and HbA1c levels can be a factor predisposing to the development of diabetic complications.
Department of Bioanalysis and Drugs Analysis, Warsaw Medical University, Warsaw, Poland.
J
J
Giebułtowicz
Department of Bioanalysis and Drugs Analysis, Warsaw Medical University, Warsaw, Poland.
S
S
Sołobodowska
Department of Laboratory Diagnostics, Medical University of Warsaw, Warsaw, Poland.
D
D
Bobilewicz
Department of Bioanalysis and Drugs Analysis, Warsaw Medical University, Warsaw, Poland.
P
P
Wroczyński
D000368:Aged; D000369:Aged, 80 and over; D000444:Aldehyde Dehydrogenase / Q000097:blood*; D000080924:Aldehyde Dehydrogenase 1 Family; D001786:Blood Glucose / Q000032:analysis; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000150:complications; D004912:Erythrocytes / Q000201:enzymology / Q000378:metabolism; D005982:Glutathione Transferase / Q000097:blood*; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans; D008875:Middle Aged; D018384:Oxidative Stress; D050697:Retinal Dehydrogenase
D000444:Aldehyde Dehydrogenase; D000080924:Aldehyde Dehydrogenase 1 Family; D001786:Blood Glucose; D005982:Glutathione Transferase; D006442:Glycated Hemoglobin; D050697:Retinal Dehydrogenase; C517652:hemoglobin A1c protein, human; C510223:ALDH1A1 protein, human