RECONSTRUCTION OF THE HUMAN HIPPOCAMPUS IN 3D FROM HISTOLOGY AND HIGH-RESOLUTION EX-VIVO MRI.
In this paper, we present methods for the reconstruction of 3D histological volumes of the human hippocampal formation from histology slices. Inter-slice alignment is guided by a graph-theoretic approach that minimizes the impact of badly distorted slices. The reconstruction is refined by iterative affine and deformable co-registration with a high-resolution MRI of the postmortem tissue sample. We present an evaluation of reconstruction accuracy that is based on measures of similarity between boundaries drawn on both histology and MRI. Our methodology is currently being applied to an MRI atlas of the human hippocampal formation, in which atlas anatomical labels are derived from segmentation of reconstructed histology.
Penn Image Computing and Science Laboratory, Department of Radiology, University of Pennsylvania, Philadelphia, PA, USA.
Daniel H
DH
Adler
Penn Image Computing and Science Laboratory, Department of Radiology, University of Pennsylvania, Philadelphia, PA, USA.
Alex Yang
AY
Liu
Penn Image Computing and Science Laboratory, Department of Radiology, University of Pennsylvania, Philadelphia, PA, USA.
John
J
Pluta
Penn Image Computing and Science Laboratory, Department of Radiology, University of Pennsylvania, Philadelphia, PA, USA.
Salmon
S
Kadivar
Penn Image Computing and Science Laboratory, Department of Radiology, University of Pennsylvania, Philadelphia, PA, USA.
Sylvia
S
Orozco
Penn Image Computing and Science Laboratory, Department of Radiology, University of Pennsylvania, Philadelphia, PA, USA.
Hongzhi
H
Wang
Penn Image Computing and Science Laboratory, Department of Radiology, University of Pennsylvania, Philadelphia, PA, USA.
James C
JC
Gee
Penn Image Computing and Science Laboratory, Department of Radiology, University of Pennsylvania, Philadelphia, PA, USA.
Brian B
BB
Avants
Penn Image Computing and Science Laboratory, Department of Radiology, University of Pennsylvania, Philadelphia, PA, USA.
Paul A
PA
Yushkevich
Hippocampus; histology; reconstruction; registration; segmentation