Neocartilage formation from mesenchymal stem cells grown in type II collagen-hyaluronan composite scaffolds.
Three-dimensional (3D) collagen type II-hyaluronan (HA) composite scaffolds (CII-HA) which mimics the extracellular environment of natural cartilage were fabricated in this study. Rheological measurements demonstrated that the incorporation of HA increased the compression modulus of the scaffolds. An initial in vitro evaluation showed that scaffolds seeded with porcine chondrocytes formed cartilaginous-like tissue after 8 weeks, and HA functioned to promote the growth of chondrocytes into scaffolds. Placenta-derived multipotent cells (PDMC) and gingival fibroblasts (GF) were seeded on tissue culture polystyrene (TCPS), CII-HA films, and small intestinal submucosa (SIS) sheets for comparing their chondrogenesis differentiation potentials with those of adipose-derived adult stem cells (ADAS) and bone marrow-derived mesenchymal stem cells (BMSC). Among different cells, PDMC showed the greatest chondrogenic differentiation potential on both CII-HA films and SIS sheets upon TGF-β3 induction, followed by GF. This was evidenced by the up-regulation of chondrogenic genes (Sox9, aggrecan, and collagen type II), which was not observed for cells grown on TCPS. This finding suggested the essential role of substrate materials in the chondrogenic differentiation of PDMC and GF. Neocartilage formation was more obvious in both PDMC and GF cells plated on CII-HA composite scaffolds vs. 8-layer SIS at 28 days in vitro. Finally, implantation of PDMC/CII-HA constructs into NOD-SCID mice confirmed the formation of tissue-engineered cartilage in vivo.
Institute of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan.
Hsi-Yi
HY
Yeh
Institute of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan.
Ting-Yu
TY
Lin
Center for Neuropsychiatry, China Medical University Hospital, Taichung, Taiwan.
Chen-Huan
CH
Lin
Institute of Cellular and System Medicine, National Health Research Institutes, Miaoli, Taiwan.
B Linju
BL
Yen
Department of Orthopaedics, National Taiwan University Hospital, Taipei, Taiwan.
Ching-Lin
CL
Tsai
Institute of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan; Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, Taipei, Taiwan; Institute of Biomedical Engineering, National Chung Hsing University, Taichung, Taiwan. Electronic address: shhsu@ntu.edu.tw.
Shan-Hui
SH
Hsu
D000328:Adult; D000818:Animals; D002356:Cartilage / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D020219:Chondrogenesis / Q000235:genetics*; D024043:Collagen Type II / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D011247:Pregnancy; D013552:Swine; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
D024043:Collagen Type II; D006820:Hyaluronic Acid
Chondrocytes; Hyaluronic acid (HA); Mesenchymal stem cells (MSCs); Neocartilage; Small intestinal submucosa (SIS); Type II collagen