Bone marrow mesenchymal stem cells ameliorate inflammatory factor-induced dysfunction of INS-1 cells on chip.
Using a microfluidic chip, we have investigated whether bone marrow mesenchymal stem cells (BM-MSCs) could ameliorate IL-1β/IFN-γ-induced dysfunction of INS-1 cells. BM-MSCs were obtained from diabetes mellitus patients and their cell surface antigen expression profiles were analyzed by flow cytometric. INS-1 cells were cocultured with BM-MSCs on a microfluidic chip with persistent perfusion of medium containing 1 ng/mL IL-1β and 2.5 U/mL IFN-γ for 72 h. BM-MSCs could partially rescue INS-1 cells from cytokine-induced dysfunction and ameliorate the expression of insulin and PDX-1 gene in INS-1 cells. Thus BM-MSCs can be viewed as a promising stem cell source to depress inflammatory factor-induced dysfunction of pancreatic β cells in diabetic patients.
Department of Endocrine, Qilu Hospital, Shandong University, Ji'nan, 250012, Shandong P.R., China.
Yu
Y
Sun
Zhina
Z
Yao
Peng
P
Lin
Xinguo
X
Hou
Li
L
Chen
D001854:Bone Marrow Cells / Q000378:metabolism; D018920:Coculture Techniques; D003920:Diabetes Mellitus / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D046210:Microfluidic Analytical Techniques / Q000379:methods*
D007328:Insulin
INS-1 cells; bone marrow; cell culture; mesenchymal stem cell; microfluidic chip