Methods for comprehensive experimental identification of RNA-protein interactions.
The importance of RNA-protein interactions in controlling mRNA regulation and non-coding RNA function is increasingly appreciated. A variety of methods exist to comprehensively define RNA-protein interactions. We describe these methods and the considerations required for designing and interpreting these experiments.
Colleen A
CA
McHugh
Pamela
P
Russell
Mitchell
M
Guttman
D000818:Animals; D005786:Gene Expression Regulation; D006801:Humans; D054730:Protein Interaction Domains and Motifs; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D022661:RNA, Untranslated / Q000235:genetics* / Q000378:metabolism; D017423:Sequence Analysis, RNA / Q000379:methods
D012333:RNA, Messenger; D022661:RNA, Untranslated