mir-300 promotes self-renewal and inhibits the differentiation of glioma stem-like cells.
MicroRNAs (miRNAs) are small noncoding RNAs that have been critically implicated in several human cancers. miRNAs are thought to participate in various biological processes, including proliferation, cell cycle, apoptosis, and even the regulation of the stemness properties of cancer stem cells. In this study, we explore the potential role of miR-300 in glioma stem-like cells (GSLCs). We isolated GSLCs from glioma biopsy specimens and identified the stemness properties of the cells through neurosphere formation assays, multilineage differentiation ability analysis, and immunofluorescence analysis of glioma stem cell markers. We found that miR-300 is commonly upregulated in glioma tissues, and the expression of miR-300 was higher in GSLCs. The results of functional experiments demonstrated that miR-300 can enhance the self-renewal of GSLCs and reduce differentiation toward both astrocyte and neural fates. In addition, LZTS2 is a direct target of miR-300. In conclusion, our results demonstrate the critical role of miR-300 in GSLCs and its functions in LZTS2 inhibition and describe a new approach for the molecular regulation of tumor stem cells.
Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Youzheng Street No. 23, Nangang District, Harbin, Heilongjiang Province, 150001, China.
Daming
D
Zhang
Guang
G
Yang
Xin
X
Chen
Chunmei
C
Li
Lu
L
Wang
Yaohua
Y
Liu
Dayong
D
Han
Huailei
H
Liu
Xu
X
Hou
Weiguang
W
Zhang
Chenguang
C
Li
Zhanqiang
Z
Han
Xin
X
Gao
Shiguang
S
Zhao
D000818:Animals; D001253:Astrocytes / Q000166:cytology; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism*; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; C440108:LZTS2 protein, human; C000596512:MIRN300 microRNA, human; D035683:MicroRNAs; D025521:Tumor Suppressor Proteins