In vitro and in vivo enhancement of adipogenesis by Italian ryegrass (Lolium multiflorum) in 3T3-L1 cells and mice.
Adipogenesis is very much important in improving the quality of meat in animals. The aim of the present study was to investigate the in vitro and in vivo adipogenesis regulation properties of Lolium multiflorum on 3T3-L1 pre-adipocytes and mice. Chemical composition of petroleum ether extract of L. multiflorum (PET-LM) confirmed the presence of fatty acids, such as α-linolenic acid, docosahexaenoic acid, oleic acid, docosatetraenoic acid, and caprylic acid, as the major compounds. PET-LM treatment increased viability, lipid accumulation, lipolysis, cell cycle progression, and DNA synthesis in the cells. PET-LM treatment also augmented peroxysome proliferator activated receptor (PPAR)-γ2, CCAAT/enhancer binding protein-α, adiponectin, adipocyte binding protein, glucose transporter-4, fatty acid synthase, and sterol regulatory element binding protein-1 expression at mRNA and protein levels in differentiated adipocytes. In addition, mice administered with 200 mg/kg body weight PET-LM for 8 weeks showed greater body weight than control mice. These findings suggest that PET-LM facilitates adipogenesis by stimulating PPARγ-mediated signaling cascades in adipocytes which could be useful for quality meat development in animals.
Grassland and Forage Division, National Institute of Animal Science, RDA, Cheonan, South Korea.
Mariadhas
M
Valan Arasu
Grassland and Forage Division, National Institute of Animal Science, RDA, Cheonan, South Korea.
Soundharrajan
S
Ilavenil
The United Graduate School of Agricultural Sciences, Tottori University, Tottori-Shi, Japan.
Da Hye
DH
Kim
Laboratory of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Sang
S
Gun Roh
Research Center of Bioactive Materials, Institute of Oral Biosciences and School of Dentistry, Chonbuk National University, Jeonju, South Korea.
Jeong-Chae
JC
Lee
Grassland and Forage Division, National Institute of Animal Science, RDA, Cheonan, South Korea.
Ki Choon
KC
Choi
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects* / Q000235:genetics; D000284:Administration, Oral; D000473:Alkanes; D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002853:Chromatography, Liquid; D004247:DNA / Q000096:biosynthesis; D019587:Dietary Supplements; D005260:Female; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects; D005990:Glycerol / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects; D008129:Lolium / Q000737:chemistry*; D013058:Mass Spectrometry; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015430:Weight Gain / Q000187:drug effects
D000473:Alkanes; D010936:Plant Extracts; D012333:RNA, Messenger; D004247:DNA; C004544:naphtha; D005990:Glycerol