Silencing of Aurora kinase A by RNA interference inhibits tumor growth in human osteosarcoma cells by inducing apoptosis and G2/M cell cycle arrest.
The overexpression of Aurora kinase A (AURKA), a member of serine/threonine kinase family, has been observed in various types of human cancers. However, the role of AURKA in osteosarcoma (OS), the most common type of primary malignancy arising from bone, has not been clarified. We used AURKA-specific lentivirus-delivered short hairpin RNA (shRNA) to significantly and sustainably silence the endogenous AURKA expression in human OS cells SAOS-2 and U2OS. We found that AURKA downregulation in OS cells prominently decreased colony formation ability in vitro and tumorigenesis ability in vivo. We further evaluated the effect of AURKA silence on cell viability by MTT assay, cell apoptosis and cell cycle by ﬂow cytometer detection. The results showed that AURKA silence inhibited cell viability by inducing cell apoptosis and G2/M cell cycle arrest in OS cells. Taken together, our findings indicate that AURKA plays a crucial role on OS growth by inhibiting cell apoptosis and propelling cell cycle. Inhibition of AURKA by lentivirus-delivered specific shRNA showed the therapeutic potential in treatment of osteosarcoma.
Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.
Zhenhuan
Z
Jiang
Department of Orthopaedics, People's Hospital of Yixing City, Yixing, Jiangsu 214200, P.R. China.
Jiannong
J
Jiang
Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.
Huilin
H
Yang
Department of Anesthesiology, People's Hospital of Yixing City, Yixing, Jiangsu 214200, P.R. China.
Zhijun
Z
Ge
Department of Orthopaedics, People's Hospital of Yixing City, Yixing, Jiangsu 214200, P.R. China.
Qiang
Q
Wang
Department of Orthopaedics, People's Hospital of Yixing City, Yixing, Jiangsu 214200, P.R. China.
Leiyan
L
Zhang
Department of Orthopaedics, People's Hospital of Yixing City, Yixing, Jiangsu 214200, P.R. China.
Chenguang
C
Wu
Department of Cell Biology, School of Medicine, Soochow University, Suzhou, Jiangsu 215123, P.R. China.
Jinzhi
J
Wang
D000818:Animals; D017209:Apoptosis; D064096:Aurora Kinase A / Q000235:genetics* / Q000378:metabolism; D001859:Bone Neoplasms / Q000201:enzymology* / Q000473:pathology; D063646:Carcinogenesis / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D059565:G2 Phase Cell Cycle Checkpoints; D055785:Gene Knockdown Techniques; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D012516:Osteosarcoma / Q000201:enzymology* / Q000473:pathology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics
D034741:RNA, Small Interfering; C576917:AURKA protein, human; D064096:Aurora Kinase A