Carotenoid production from n-alkanes with a broad range of chain lengths by the novel species Gordonia ajoucoccus A2(T).
A novel diesel-degrading bacterial strain, A2(T), was isolated from soil that was heavily contaminated with oil. Based on phenotypic, phylogenetic, and DNA analyses, strain A2(T) was identified as a novel species of the genus Gordonia and named Gordonia ajoucoccus A2(T) (KCTC 11900BP and CECT8382). G. ajoucoccus A2(T) is able to synthesize carotenoids and produces mainly γ-carotene and keto-γ-carotene. G. ajoucoccus A2(T) is also capable of assimilating n-alkanes with a broad range of chain lengths (C6, C8-C25). Batch culture of G. ajoucoccus A2(T) in a bioreactor containing 1 % (v/v) hexadecane or 1 % (v/v) commercial diesel yielded 25 mg L⁻¹ and 2.6 mg L⁻¹ of carotenoids, respectively. Gas chromatography/mass spectrometry (GC-MS) analysis of hexadecane and hexane degradation metabolites suggested that G. ajoucoccus A2(T) may possess a terminal oxidation pathway that allows it to utilize n-alkanes and hexane as carbon and energy sources. G. ajoucoccus A2(T) could therefore serve as a good model system for understanding microbial n-alkane degradation pathways. Additionally, the metabolic capabilities of G. ajoucoccus A2(T) suggest potential biotechnological applications, such as the bioproduction of carotenoids from industrial discharge or other sources of n-alkanes.
Department of Molecular Science and Technology, Department of Applied Chemistry and Biological Engineering, Ajou University, Woncheon-dong, Yeongtong-gu, Suwon, 443-749, Republic of Korea.
Jin Ho
JH
Kim
Se Hyeuk
SH
Kim
Jeong Hoon
JH
Yoon
Pyung Cheon
PC
Lee
D000192:Actinomycetales / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000473:Alkanes / Q000378:metabolism*; D001711:Biotransformation; D002338:Carotenoids / Q000378:metabolism*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D053858:Metabolic Networks and Pathways; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D000473:Alkanes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D002338:Carotenoids