Biocompatibility, biodegradation, and neovascularization of human single-unit platelet-rich fibrin glue: an in vivo analysis.
BACKGROUND
The clinical applications of fibrin glue span over several surgical modalities. The aim of this study was to evaluate the biocompatibility and biodegradation of different formulations of platelet-rich fibrin glue in vivo and examine its effects on the neovascularization of wound sites.


METHODS
Human-derived single-unit fibrin glue was prepared. Incisions were made on the backs of rats, and these were coated with homemade glues containing different concentrations of aminomethylbenzoic acid (Groups A-F) or commercial adhesives (Group G). A sham control group was included (Group H). The wounds were examined by histological analysis and immunohistochemistry at several time points.


RESULTS
Successful wound closure was achieved in all groups by day 12. Acute inflammation occurred during the first six days, but gradually disappeared. The longest sealant duration was achieved using the lowest concentration of anti-fibrinolytic agent in a 1:10 volume ratio with cryoprecipitate. Expression levels of the platelet endothelial cell adhesion molecule-1 were significantly higher in Groups A and C compared to the control groups (Groups G and H) on day 3 (P < 0.05).


CONCLUSIONS
Single-unit platelet-rich fibrin glue has excellent biocompatibility and is associated with the upregulation of neovascularization. The addition of aminomethylbenzoic acid could prevent the degradation of fibrin glue.
Department of Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210002, China.
Xiuwen
X
Wu
Department of Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210002, China. Email: jiananr@gmail.com).
Jianan
J
Ren
Department of Transfusion, Jinling Hospital, Nanjing, Jiangsu 210002, China.
Genhong
G
Yao
Department of Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210002, China.
Bo
B
Zhou
Department of Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210002, China.
Gefei
G
Wang
Department of Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210002, China.
Guosheng
G
Gu
Department of Transfusion, Jinling Hospital, Nanjing, Jiangsu 210002, China.
Jianfeng
J
Luan
Department of Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210002, China.
Jieshou
J
Li
D000818:Animals; D005260:Female; D015718:Fibrin Tissue Adhesive / Q000009:adverse effects / Q000627:therapeutic use*; D006801:Humans; D007150:Immunohistochemistry; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014945:Wound Healing / Q000187:drug effects
D015718:Fibrin Tissue Adhesive; D019408:Platelet Endothelial Cell Adhesion Molecule-1