Guided bone regeneration using chitosan-collagen membranes in dog dehiscence-type defect model.
OBJECTIVE
The purpose of the present study was to compare a newly developed chitosan-collagen membrane (CCM) with a standard collagen membrane (SCM) regarding their effects on guided bone regeneration.


METHODS
The right mandibular premolars and first molar were extracted from 12 beagle dogs. Four months later, acute buccal dehiscence-type defects (4 × 3 mm in height and width) were surgically created after implant site preparation. The defects were randomly assigned to 4 different groups: CCM-1 (weight ratio of chitosan to collagen of 40:1), CCM-2 (weight ratio of chitosan to collagen of 20:1), SCM, and vehicle control. The dogs were sacrificed after 4, 8, and 12 weeks of healing for radiographic examination, histologic observation, and histometric analysis.


RESULTS
The membrane-treated sites showed more bone formation than the control sites, although no statistically significant differences were found between the membrane-treated sites and the control sites for new bone-to-implant contact and new bone-filled area at any point. At 8 weeks, the new bone height for the membrane-treated sites was significantly greater statistically than that of the untreated group (P < .05). At 12 weeks, the CCM-1 group showed significantly greater new bone height (1.91 ± 0.25 mm) than the untreated group (1.20 ± 0.34 mm; P < .05). However, the CCMs did not show any statistically significant differences compared with the SCMs for any assessed parameter.


CONCLUSIONS
The results of the present study have shown that the developed CCMs can enhance bone regeneration and could be a candidate for use in guided bone regeneration.
MD Student, Department of Prosthetic Dentistry, Second Affiliated Hospital, Zhejiang University College of Medicine, Hangzhou, China.
Xiaojing
X
Li
Associate Professor, Department of Oral Surgery, First People's Hospital of Hangzhou, Hangzhou, China.
Xinmu
X
Wang
Resident, Department of Orthopedic Surgery, Second Affiliated Hospital (Binjiang Branch), Hangzhou Binjiang Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Tengfei
T
Zhao
MD Student, Department of Prosthetic Dentistry, Second Affiliated Hospital, Zhejiang University College of Medicine, Hangzhou, China.
Bo
B
Gao
MD Student, Department of Prosthetic Dentistry, Second Affiliated Hospital, Zhejiang University College of Medicine, Hangzhou, China.
Yuwen
Y
Miao
MD Student, Department of Prosthetic Dentistry, Second Affiliated Hospital, Zhejiang University College of Medicine, Hangzhou, China.
Dandan
D
Zhang
Associate Professor, Department of Prosthetic Dentistry, Second Affiliated Hospital, Zhejiang University College of Medicine, Hangzhou, China. Electronic address: yandong_66@163.com.
Yan
Y
Dong
D000818:Animals; D001861:Bone Regeneration; D048271:Chitosan; D003094:Collagen; D003758:Dental Implantation, Endosseous; D004285:Dogs; D048091:Guided Tissue Regeneration / Q000379:methods*; D008567:Membranes, Artificial; D016348:Osseointegration; D011897:Random Allocation; D013529:Surgical Wound Dehiscence; D014081:Tooth Extraction; D020390:Tooth Socket / Q000601:surgery*
D008567:Membranes, Artificial; D003094:Collagen; D048271:Chitosan