Optimization of polygalacturonase production from a newly isolated Thalassospira frigidphilosprofundus to use in pectin hydrolysis: statistical approach.
The present study deals with the production of cold active polygalacturonase (PGase) by submerged fermentation using Thalassospira frigidphilosprofundus, a novel species isolated from deep waters of Bay of Bengal. Nonlinear models were applied to optimize the medium components for enhanced production of PGase. Taguchi orthogonal array design was adopted to evaluate the factors influencing the yield of PGase, followed by the central composite design (CCD) of response surface methodology (RSM) to identify the optimum concentrations of the key factors responsible for PGase production. Data obtained from the above mentioned statistical experimental design was used for final optimization study by linking the artificial neural network and genetic algorithm (ANN-GA). Using ANN-GA hybrid model, the maximum PGase activity (32.54 U/mL) was achieved at the optimized concentrations of medium components. In a comparison between the optimal output of RSM and ANN-GA hybrid, the latter favored the production of PGase. In addition, the study also focused on the determination of factors responsible for pectin hydrolysis by crude pectinase extracted from T. frigidphilosprofundus through the central composite design. Results indicated 80% degradation of pectin in banana fiber at 20 °C in 120 min, suggesting the scope of cold active PGase usage in the treatment of raw banana fibers.
Department of Biotechnology, R. V. R. & J. C. College of Engineering, Chowdavaram, Guntur, India.
V P B
VP
Rekha
Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh 522510, India.
Mrinmoy
M
Ghosh
Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh 522510, India.
Vijayanand
V
Adapa
Department of Animal Biotechnology, School of Applied Life Science, Faculty of Biotechnology, Jeju National University, Jeju City 690-756, Republic of Korea.
Sung-Jong
SJ
Oh
Center for Bioseparation Technology, VIT University, Vellore, Tamil Nadu 632014, India.
K K
KK
Pulicherla
Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh 522510, India.
K R S
KR
Sambasiva Rao
D003470:Culture Media; D005285:Fermentation; D006868:Hydrolysis; D010368:Pectins / Q000096:biosynthesis*; D011096:Polygalacturonase / Q000096:biosynthesis*; D012244:Rhodospirillaceae / Q000201:enzymology / Q000254:growth & development
D003470:Culture Media; D010368:Pectins; D011096:Polygalacturonase