Preparation, blood coagulation and cell compatibility evaluation of chitosan-graft-polylactide copolymers.
Biodegradable chitosan-graft-polylactide (PLA-CS) copolymers were prepared by the grafting of a poly(L-lactide) (PLLA) or poly(D-lactide) (PDLA) precursor to the backbone of chitosan using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC ⋅ HCl) and N-hydroxysuccinimide (NHS) as a coupling agent. The blood and cell compatibility of the graft copolymers were investigated in comparison to PLLA and PDLA homopolymers. The coagulation properties of PLA-CS were evaluated by hemolysis, plasma recalcification, dynamic blood clotting and protein absorption assays. PLA-CS copolymers present similar hemolysis ratio and plasma recalcification time as PLA, but slower dynamic blood clotting and lower protein absorption. The cell viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), agar diffusion and lactate dehydrogenase (LDH) experiments. All the samples presented no effect on the viability to cells. Inflammatory cytokine analysis using sandwich ELISAs revealed that PLA-CS would not stimulate inflammatory activity.
Key Laboratory of Drug Targeting and Novel Drug Delivery Systems, Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu 610041, People's Republic of China. State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200032, People's Republic of China.
Qi
Q
Wang
Pei
P
Liu
Peifeng
P
Liu
Tao
T
Gong
Suming
S
Li
Yourong
Y
Duan
Zhirong
Z
Zhang
D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001777:Blood Coagulation; D002234:Carbodiimides / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry*; D004058:Diffusion; D004797:Enzyme-Linked Immunosorbent Assay; D006461:Hemolysis; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007249:Inflammation; D008422:Materials Testing; D008744:Methylamines / Q000737:chemistry*; D051379:Mice; D011091:Polyesters / Q000737:chemistry*; D013388:Succinimides / Q000737:chemistry*; D013778:Tetrazolium Salts / Q000737:chemistry; D013844:Thiazoles / Q000737:chemistry
C569779:1-(3-dimethylaminopropyl)-dimethylaminopropyl-ethylcarbodiimide hydrochloride; D001672:Biocompatible Materials; D002234:Carbodiimides; D008744:Methylamines; D011091:Polyesters; D013388:Succinimides; D013778:Tetrazolium Salts; D013844:Thiazoles; C033616:poly(lactide); D048271:Chitosan; C022616:thiazolyl blue; C001426:N-hydroxysuccinimide