Immunoblotting of soluble antigens in Paracoccidioides brasiliensis culture.
This study investigated the major soluble antigens produced by Paracoccidioides brasiliensis (Pb339) cultured in solid Sabouraud (pH 5.6 and 8.5), Sabouraud plus brain heart infusion and liquid tomato juice-enriched complex medium media at intervals of 3 days over 30 days by immunoblotting and concluded that, to optimize the source of each antigen, both time and growth conditions should be considered.
Department of Pathologic Sciences, State University of Londrina, Londrina, Parana, Brazil.
Nilson
N
de Jesus Carlos
Débora Alves
DA
Pinto
Mario Augusto
MA
Ono
Emerson Jose
EJ
Venancio
Zoilo Pires
ZP
de Camargo
Ayako
A
Sano
Eiko Nakagawa
EN
Itano
D000946:Antigens, Fungal / Q000032:analysis* / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D015151:Immunoblotting; D010228:Paracoccidioides / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism
D000946:Antigens, Fungal; D003470:Culture Media
fungi; immunodiagnosis; paracoccidioidomycosis; virulence factor