Flow cytometry applications for the analysis of chemokine receptor expression and function.
Chemokine receptors play an important role in leukocyte migration, both in physiological and pathological conditions, and the interest in new methodologies for their detection is increasing. In this review, we focused on chemokine receptors detection through flow cytometric approaches, including the use of specific antibodies and fluorescent chemokines, and on approaches aimed at the analysis of their functions, from intracellular trafficking to signaling activities.
Humanitas Clinical and Research Center, Rozzano, Milan, 20089, Italy.
Achille
A
Anselmo
Cristina
C
Mazzon
Elena Monica
EM
Borroni
Raffaella
R
Bonecchi
Gerard J
GJ
Graham
Massimo
M
Locati
D000818:Animals; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007962:Leukocytes / Q000378:metabolism; D019707:Receptors, Chemokine / Q000032:analysis*
D019707:Receptors, Chemokine
antibodies; chemokine receptor; flow cytometry; fluorophores