Chemical fingerprinting and quantitative analysis of two common Gleditsia sinensis fruits using HPLC-DAD.
Gleditsiae Fructus Abnormalis and Gleditsiae Sinensis Fructus are obtained from different developmental stages of fruits from Gleditsia sinensis Lam. (Leguminosae). The possible interchangeable usage of the two fruits, however, has long been very controversial. Here, high performance liquid chromatography coupled with diode array detection was developed to explore their chemical fingerprinting profiles. Besides, the amounts of aglycones of saponin compounds, echinocystic acid and oleanolic acid in both fruits were quantified. The results indicated that there was no significant difference in the content of aglycones from the two types of fruits. However, their chromatographic fingerprints showed distinct characteristics. Therefore, the interchangeable application of these fruits has to be taken with a specific precaution.
Jianping
J
Chen
Zhonggui
Z
Li
Ken Y Z
KY
Zheng
Ava J Y
AJ
Guo
Kevin Y
KY
Zhu
Wendy L
WL
Zhang
Janis Y X
JY
Zhan
Tina T X
TT
Dong
Ziren
Z
Su
Karl W K
KW
Tsim
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005638:Fruit; D031501:Gleditsia / Q000737:chemistry*; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification*
D012503:Saponins; D009828:Oleanolic Acid; C022999:echinocystic acid