Defining the region of Bacillus subtilis SpoIIIJ that is essential for its sporulation-specific function.
Proteins of the YidC/OxaI/Alb3 family play a crucial role in the insertion, folding, and/or assembly of membrane proteins in prokaryotes and eukaryotes. Bacillus subtilis has two YidC-like proteins, denoted SpoIIIJ and YqjG. SpoIIIJ and YqjG are largely exchangeable in function, but SpoIIIJ has a unique role in sporulation, while YqjG stimulates competence development. To obtain more insight into the regions important for the sporulation specificity of SpoIIIJ, a series of SpoIIIJ/YqjG chimeras was constructed. These chimeras were tested for functionality during vegetative growth and for their ability to complement the sporulation defect of a spoIIIJ deletion strain. The data suggest an important role for the domain comprising transmembrane segment 2 (TMS2) and its flanking loops in sporulation specificity, with lesser contributions to specificity by TMS1 and TMS3.
Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and Zernike Institute for Advanced Materials, Groningen, The Netherlands.
Yanping
Y
Geng
Jeanine
J
de Keyzer
Dirk-Jan
DJ
Scheffers
Arnold J M
AJ
Driessen
D001412:Bacillus subtilis / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D013171:Spores, Bacterial / Q000737:chemistry / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism
D001426:Bacterial Proteins; D008565:Membrane Proteins; C030069:spore-specific proteins, Bacillus