A novel MYB transcription factor, GmMYBJ1, from soybean confers drought and cold tolerance in Arabidopsis thaliana.
MYB transcription factors play important roles in the regulation of plant growth, developmental metabolism and stress responses. In this study, a new MYB transcription factor gene, GmMYBJ1, was isolated from soybean [Glycine max (L.)]. The GmMYBJ1 cDNA is 1296bp in length with an open reading frame (ORF) of 816 bp encoding for 271 amino acids. The amino acid sequence displays similarities to the typical R2R3 MYB proteins reported in other plants. Transient expression analysis using the GmMYBJ1-GFP fusion gene in onion epidermal cells revealed that the GmMYBJ1 protein is targeted to the nucleus. Quantitative RT-PCR analysis demonstrated that GmMYBJ1 expression was induced by abiotic stresses, such as drought, cold, salt and exogenous abscisic acid (ABA). Compared to wild-type (WT) plants, transgenic Arabidopsis overexpressing GmMYBJ1 exhibited an enhanced tolerance to drought and cold stresses. These results indicate that GmMYBJ1 has the potential to be utilized in transgenic breeding lines to improve abiotic stress tolerance.
Jilin Key Laboratory for Crop Genetic Engineering, College of Plant Science, Jilin University, Changchun 130062, Jilin, China.
Lian-Tai
LT
Su
Jilin Key Laboratory for Crop Genetic Engineering, College of Plant Science, Jilin University, Changchun 130062, Jilin, China.
Jing-Wen
JW
Li
Jilin Key Laboratory for Crop Genetic Engineering, College of Plant Science, Jilin University, Changchun 130062, Jilin, China.
De-Quan
DQ
Liu
College of Life Science and Agroforestry, Qiqihaer University, Qiqihaer 161006, Heilongjiang, China.
Ying
Y
Zhai
Jilin Key Laboratory for Crop Genetic Engineering, College of Plant Science, Jilin University, Changchun 130062, Jilin, China.
Hai-Jun
HJ
Zhang
Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130012, Jilin, China.
Xiao-Wei
XW
Li
Jilin Key Laboratory for Crop Genetic Engineering, College of Plant Science, Jilin University, Changchun 130062, Jilin, China.
Qing-Lin
QL
Zhang
Jilin Key Laboratory for Crop Genetic Engineering, College of Plant Science, Jilin University, Changchun 130062, Jilin, China. Electronic address: wangying2009@jlu.edu.cn.
Ying
Y
Wang
Jilin Key Laboratory for Crop Genetic Engineering, College of Plant Science, Jilin University, Changchun 130062, Jilin, China. Electronic address: qywang@jlu.edu.cn.
Qing-Yu
QY
Wang
D021581:Active Transport, Cell Nucleus; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D002467:Cell Nucleus / Q000378:metabolism; D003080:Cold Temperature; D003681:Dehydration; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013025:Glycine max / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D013312:Stress, Physiological; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019076:Transgenes
D010940:Plant Proteins; D014157:Transcription Factors
MYB transcription factor; Soybean; Stress tolerance; Transgenic Arabidopsis