Gold nanoparticle amplified optical microfiber evanescent wave absorption biosensor for cancer biomarker detection in serum.
Sensitive and selective detection for cancer biomarkers is critical in cancer clinical diagnostics. In this work, we report a new optical microfiber (OMF) biosensor using gold nanoparticles (GNPs) as amplification labels for the detection of alpha-fetoprotein (AFP) in serum samples. By combining the unique optical property of OMFs and the strong optical absorption of GNPs, very high sensitivity and selectivity can be achieved. Critical parameters namely fiber diameter and GNP size were optimized for better performance. The limit of detection (LOD) of this sensor for AFP is 0.2 ng/mL in PBS and 2 ng/mL in bovine serum, which is comparable to conventional assays. The advantages of this biosensor are simple detection scheme, fast response time, and ease of miniaturization, which might make this biosensor a promising platform for clinical cancer diagnosis and prognosis.
State Key Laboratory of Applied Optics, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, PR China; University of Chinese Academy of Sciences, Beijing 100039, PR China.
Kaiwei
K
Li
State Key Laboratory of Applied Optics, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, PR China.
Guigen
G
Liu
State Key Laboratory of Applied Optics, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, PR China. Electronic address: yihuiwu@ciomp.ac.cn.
Yihui
Y
Wu
State Key Laboratory of Applied Optics, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, PR China.
Peng
P
Hao
State Key Laboratory of Applied Optics, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, PR China; University of Chinese Academy of Sciences, Beijing 100039, PR China.
Wenchao
W
Zhou
Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, PR China.
Zhiqiang
Z
Zhang
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D002417:Cattle; D004867:Equipment Design; D005336:Fiber Optic Technology / Q000295:instrumentation*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000097:blood / Q000175:diagnosis; D000509:alpha-Fetoproteins / Q000032:analysis*
D000509:alpha-Fetoproteins; D006046:Gold
Alpha-fetoprotein (AFP); Cancer biomarker; Fiber optic sensor; Gold nanoparticle; Optical microfiber