Potential of multispectral imaging technology for rapid and non-destructive determination of the microbiological quality of beef filets during aerobic storage.
The performance of a multispectral imaging system has been evaluated in monitoring aerobically packaged beef filet spoilage at different storage temperatures (0, 4, 8, 12, and 16°C). Spectral data in the visible and short wave near infrared area (405-970nm) were collected from the surface of meat samples and correlated with microbiological data (log counts), for total viable counts (TVCs), Pseudomonas spp., and Brochothrix thermosphacta. Qualitative analysis (PLS-DA) was employed for the discrimination of meat samples in three microbiological quality classes based on the values of total viable counts, namely Class 1 (TVC<5.5log10CFU/g), Class 2 (5.5log10CFU/g<TVC<7.0log10CFU/g), and Class 3 (TVC>7.0log10CFU/g). Furthermore, PLS regression models were developed to provide quantitative estimations of microbial counts during meat storage. In both cases model validation was implemented with independent experiments at intermediate storage temperatures (2 and 10°C) using different batches of meat. Results demonstrated good performance in classifying meat samples with overall correct classification rate for the three quality classes ranging from 91.8% to 80.0% for model calibration and validation, respectively. For quantitative estimation, the calculated regression coefficients between observed and estimated counts ranged within 0.90-0.93 and 0.78-0.86 for model development and validation, respectively, depending on the microorganism. Moreover, the calculated average deviation between observations and estimations was 11.6%, 13.6%, and 16.7% for Pseudomonas spp., B. thermosphacta, and TVC, respectively. The results indicated that multispectral vision technology has significant potential as a rapid and non-destructive technique in assessing the microbiological quality of beef fillets.
Laboratory of Microbiology and Biotechnology of Foods, Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, Athens GR-11855, Greece. Electronic address: stathispanagou@aua.gr.
Efstathios Z
EZ
Panagou
Laboratory of Microbiology and Biotechnology of Foods, Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, Athens GR-11855, Greece.
Olga
O
Papadopoulou
DTU Compute, Technical University of Denmark, Richard Petersens Plads 321, Kgs. Lyngby DK-2800, Denmark.
Jens Michael
JM
Carstensen
Laboratory of Microbiology and Biotechnology of Foods, Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, Athens GR-11855, Greece.
George-John E
GJ
Nychas
D000818:Animals; D058640:Brochothrix / Q000502:physiology; D002138:Calibration; D002417:Cattle; D015169:Colony Count, Microbial; D005511:Food Handling; D005516:Food Microbiology / Q000379:methods*; D008460:Meat / Q000382:microbiology*; D061848:Optical Imaging / Q000592:standards*; D011549:Pseudomonas / Q000502:physiology; D012044:Regression Analysis; D015203:Reproducibility of Results; D013696:Temperature
Aerobic storage; Chemometrics; Meat spoilage; Multispectral vision technology; Non-invasive methods