Development of an improved time-resolved fluoroimmunoassay for simultaneous quantification of C-peptide and insulin in human serum.
OBJECTIVE
Diabetes mellitus is a chronic disease affecting millions of people globally and resulting in significant death rates each year. A fast, inexpensive alternative to traditional testing and monitoring techniques is desirable, since secretion of insulin and C-peptide is impaired in diabetes mellitus.


METHODS
A highly sensitive immunoassay was developed for the simultaneous measurement of C-peptide and insulin levels in human serum, utilizing dual-label time-resolved fluoroimmunoassay (TRFIA) and magnetic particle technologies. This assay was characteristic for a single-step sandwich-type immunoassay, wherein antibody-coated magnetic particles were used as the solid phase and Eu(3+) and Sm(3+) chelate labels were used for detection.


RESULTS
Antibody-coated magnetic particles in a TRFIA format performed well in addressing a number of quantitative needs.


CONCLUSIONS
The results of this assay correlated well with commercial chemiluminescence assays and provided a number a advantages, including reduced sample volume, reduced reagent and personnel costs and reduced assay time, while maintaining the required clinical sensitivity.
Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou 510515, PR China.
Tian-Cai
TC
Liu
Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou 510515, PR China.
Mei-Jun
MJ
Chen
Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou 510515, PR China.
Zhi-Qi
ZQ
Ren
Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou 510515, PR China.
Jing-Yuan
JY
Hou
Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou 510515, PR China.
Guan-Feng
GF
Lin
Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou 510515, PR China. Electronic address: wg@smu.edu.cn.
Ying-Song
YS
Wu
D000906:Antibodies / Q000378:metabolism; D002096:C-Peptide / Q000097:blood*; D002138:Calibration; D003920:Diabetes Mellitus / Q000097:blood; D015200:Fluoroimmunoassay / Q000379:methods*; D005934:Glucagon / Q000494:pharmacology; D006801:Humans; D007328:Insulin / Q000097:blood*; D057230:Limit of Detection; D060328:Magnetic Phenomena; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
D000906:Antibodies; D002096:C-Peptide; D007328:Insulin; D005934:Glucagon
C-peptide; Insulin; Magnetic particles; Simultaneous quantification; Time-resolved fluoroimmunoassay