Ecotoxicity of single-wall carbon nanotubes to freshwater snail Lymnaea luteola L.: Impacts on oxidative stress and genotoxicity.
Mammalian studies have raised concerns about the toxicity of carbon nanotubes, but there is very limited data on ecogenotoxicity to aquatic organisms. The aim of this study was to determine eco-geno toxic effects of single walled carbon nanotubes (SWCNTs) in fresh water snail, Lymnea luteola (L. luteola). A static test system was used to expose L. luteola to a freshwater control, 0.05, 0.15, 0.30, 0.46 mg/L SWCNTs for up to 4 days. SWCNTs changed a significant reduction in glutathione, glutathione-S-transferase, and glutathione peroxidase with in hepatopancreas of L. luteola. Lipid peroxidation (LPO) and catalase showed dose- and time-dependent and statistically significant increase in hepatopancreas during SWCNTs exposure compared with control. However, a significant (p < 0.01) induction in DNA damage was observed by the comet assay in hepatopancreas cells treated with SWCNTs. These results demonstrate that SWCNTs are ecogenotoxic to freshwater snail L. luteola. The oxidative stress and comet assay can successfully be used as sensitive tools of aquatic pollution biomonitoring.
Department of Zoology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.
Daoud
D
Ali
Department of Zoology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.
Mukhtar
M
Ahmed
Department of Zoology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.
Saud
S
Alarifi
Department of Chemistry, Maulana Azad National Institute of Technology, Bhopal, Madhya Pradesh, India.
Huma
H
Ali
D000818:Animals; D001522:Behavior, Animal; D002374:Catalase / Q000378:metabolism; D020552:Comet Assay; D004249:DNA Damage; D005618:Fresh Water; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase; D006458:Hemolymph / Q000187:drug effects; D043143:Hepatopancreas / Q000473:pathology; D007928:Lethal Dose 50; D015227:Lipid Peroxidation / Q000187:drug effects; D008195:Lymnaea / Q000187:drug effects*; D009153:Mutagens / Q000633:toxicity*; D037742:Nanotubes, Carbon / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism
D009153:Mutagens; D037742:Nanotubes, Carbon; D017382:Reactive Oxygen Species; D002374:Catalase; D005979:Glutathione Peroxidase; D005978:Glutathione
DNA damage; Lymnea luteola L; comet assay; oxidative stress; single walled carbon nanotubes