miR-132 targeting cyclin E1 suppresses cell proliferation in osteosarcoma cells.
In this study, we investigated the roles of miR-132 in tumor growth of osteosarcoma. We found that overexpression of miR-132 significantly suppressed in vitro cell proliferation and in vivo tumor growth. In addition, miR-132 overexpression induced G1/S cell cycle arrest of osteosarcoma cells. Further study showed that miR-132 could interact with the 3'-untranslated region of cyclin E1 (CCNE1) gene and repress its expression. Re-expression of CCNE1 (without the 3'UTR) could partially abrogate the miR-132-induced cell proliferation inhibition. Of significance, contrary to CCNE1, expression level of miR-132 was significantly lower in osteosarcoma tissues than in the adjacent normal tissues. Taken together, these results indicate that miR-132 functions as a tumor suppressor in osteosarcoma and that its suppressive effects are mediated chiefly by repressing CCNE1 expression.
Department of Orthopedics, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai, 200433, China.
Jin
J
Wang
Guoxing
G
Xu
Feng
F
Shen
Yifan
Y
Kang
D000818:Animals; D001859:Bone Neoplasms / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019927:Cyclin E / Q000235:genetics*; D016147:Genes, Tumor Suppressor / Q000502:physiology; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000502:physiology*; D015513:Oncogene Proteins / Q000235:genetics*; D012516:Osteosarcoma / Q000235:genetics / Q000473:pathology*
C475820:CCNE1 protein, human; D019927:Cyclin E; C547077:MIRN132 microRNA, human; D035683:MicroRNAs; D015513:Oncogene Proteins