Flow cytometric differentiation of abnormal and normal plasma cells in the bone marrow in patients with multiple myeloma and its precursor diseases.
Flow cytometric (FC) enumeration of abnormal plasma cells (APCs) for diagnosis and prognostication of plasma cell dyscrasias (PCD) is challenging. We studied antigen expression in normal plasma cells (NPC) (N = 34) and APC in a series of unselected PCD (N = 59). NPC subpopulations often demonstrated CD19(-), CD20(+), CD45(-) or dim and CD56(+), an immunophenotype observed in PCD. However abnormal CD81 was only observed in APCs (APC detection sensitivity 95%; specificity 100%). We evaluated differences in antigen expression patterns among MGUS (N = 14), SMM (N = 35) and MM (N = 10), finding the combination of CD45 and CD56 helpful in differentiating MGUS from SMM and MM (p = 0.0002).
Flow Cytometry Laboratory, Laboratory of Pathology, CCR, NCI, NIH, Bethesda, MD, USA.
Prashant R
PR
Tembhare
Flow Cytometry Laboratory, Laboratory of Pathology, CCR, NCI, NIH, Bethesda, MD, USA.
Constance M
CM
Yuan
Biostatistics and Data Management Section, CCR, NCI, NIH, Bethesda, MD, USA.
David
D
Venzon
Department of Laboratory Medicine, Clinical Center, NIH, Bethesda, MD, USA.
Raul
R
Braylan
Medical Oncology Branch, National Cancer Institute, NIH, Bethesda, MD, USA.
Neha
N
Korde
Medical Oncology Branch, National Cancer Institute, NIH, Bethesda, MD, USA.
Elisabet
E
Manasanch
Metabolism Branch, National Cancer Institute, NIH, Bethesda, MD, USA.
Diamond
D
Zuchlinsky
Department of Laboratory Medicine, Clinical Center, NIH, Bethesda, MD, USA.
Katherine
K
Calvo
Department of Laboratory Medicine, Clinical Center, NIH, Bethesda, MD, USA.
Roger
R
Kurlander
Metabolism Branch, National Cancer Institute, NIH, Bethesda, MD, USA.
Manisha
M
Bhutani
Metabolism Branch, National Cancer Institute, NIH, Bethesda, MD, USA.
Nishant
N
Tageja
Department of Laboratory Medicine, Clinical Center, NIH, Bethesda, MD, USA.
Irina
I
Maric
Metabolism Branch, National Cancer Institute, NIH, Bethesda, MD, USA.
Marcia
M
Mulquin
Metabolism Branch, National Cancer Institute, NIH, Bethesda, MD, USA.
Mark
M
Roschewski
Medical Oncology Branch, National Cancer Institute, NIH, Bethesda, MD, USA.
Mary
M
Kwok
Biostatistics and Data Management Section, CCR, NCI, NIH, Bethesda, MD, USA.
David
D
Liewehr
Metabolism Branch, National Cancer Institute, NIH, Bethesda, MD, USA.
Ola
O
Landgren
Flow Cytometry Laboratory, Laboratory of Pathology, CCR, NCI, NIH, Bethesda, MD, USA. Electronic address: stetler@mail.nih.gov.
Maryalice
M
Stetler-Stevenson
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001853:Bone Marrow / Q000276:immunology / Q000473:pathology*; D019002:CD56 Antigen / Q000235:genetics / Q000276:immunology; D002454:Cell Differentiation; D003937:Diagnosis, Differential; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D016130:Immunophenotyping; D017493:Leukocyte Common Antigens / Q000235:genetics / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008998:Monoclonal Gammopathy of Undetermined Significance / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D009101:Multiple Myeloma / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D010265:Paraproteinemias / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D010950:Plasma Cells / Q000276:immunology / Q000473:pathology*; D060225:Tetraspanin 28 / Q000235:genetics / Q000276:immunology
D019002:CD56 Antigen; C554798:CD81 protein, human; C522918:NCAM1 protein, human; D060225:Tetraspanin 28; D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human
CD81; Monoclonal gammopathy of uncertain significance; Multiple myeloma; Normal plasma cell immunophenotype; Smoldering multiple myeloma