In vivo optogenetic identification and manipulation of GABAergic interneuron subtypes.
Identification and manipulation of different GABAergic interneuron classes in the behaving animal are important to understand their role in circuit dynamics and behavior. The combination of optogenetics and large-scale neuronal recordings allows specific interneuron populations to be identified and perturbed for circuit analysis in intact animals. A crucial aspect of this approach is coupling electrophysiological recording with spatially and temporally precise light delivery. Focal multisite illumination of neuronal activators and silencers in predetermined temporal configurations or a closed loop manner opens the door to addressing many novel questions. Recent progress demonstrates the utility and power of this novel technique for interneuron research.
NYU Neuroscience Institute, School of Medicine and Center for Neural Science, New York University, New York, NY 10016, United States.
Lisa
L
Roux
NYU Neuroscience Institute, School of Medicine and Center for Neural Science, New York University, New York, NY 10016, United States.
Eran
E
Stark
NYU Neuroscience Institute, School of Medicine and Center for Neural Science, New York University, New York, NY 10016, United States; Department of Psychiatry, New York University Medical Center, New York, NY 10016, United States.
Lucas
L
Sjulson
NYU Neuroscience Institute, School of Medicine and Center for Neural Science, New York University, New York, NY 10016, United States. Electronic address: gyorgy.buzsaki@nyumc.org.
György
G
Buzsáki
D000818:Animals; D001921:Brain / Q000166:cytology; D059330:GABAergic Neurons / Q000145:classification* / Q000502:physiology*; D007395:Interneurons / Q000145:classification* / Q000378:metabolism*; D010775:Photic Stimulation; D061088:Photoacoustic Techniques