CCL28 involvement in mucosal tissues protection as a chemokine and as an antibacterial peptide.
CCL28 chemokine is expressed by epithelial cells of various mucosal tissues. This chemokine binds to CCR3 and CCR10 receptors and plays an essential role in the IgA antibody secreting cells (IgA-ASC) homing to mucosal surfaces and to lactating mammary gland as well. In addition, CCL28 has been shown to exert a potent antimicrobial activity against both Gram-negative and Gram-positive bacteria and fungi. Using the pig model, we investigated the expression of both CCR10 and CCR3 receptors in a large panel of mucosal tissues. RT-PCR analysis revealed the expression of CCR3 and CCR10 mRNA in salivary glands, nasal mucosae, Peyer's patches, small and large intestine, suggesting the presence of leucocytes expressing these receptors within these tissues. CCR10 mRNA was observed in sow mammary gland at late gestation with an increasing level during lactation. Recombinant porcine CCL28 protein was produced and mass spectrometry analysis revealed antimicrobial chemokines features such as a high pI value (10.2) and a C-terminal highly positively-charged region. Using a viable count assay, we showed that CCL28 displayed antimicrobial activity against enteric pathogens and was effective in killing Salmonella serotypes Dublin and Choleraesuis, enteroinvasive Escherichia coli K88 and non-pathogenic E. Coli K12. The potent antimicrobial function of CCL28 combined with its wide distribution in mucosal tissues and secretions suggest that this protein plays an important role in innate immune protection of the epithelial surfaces.
INRA Centre de Val de Loire, UMR-1282 Infectiologie et Santé Publique, F-37380 Nouzilly, France. Electronic address: mberri@tours.inra.fr.
Mustapha
M
Berri
INRA Centre de Val de Loire, UMR-1282 Infectiologie et Santé Publique, F-37380 Nouzilly, France. Electronic address: ipayant@tours.inra.fr.
Isabelle
I
Virlogeux-Payant
INRA Centre de Val de Loire, UMR-1282 Infectiologie et Santé Publique, F-37380 Nouzilly, France. Electronic address: chevaley@tours.inra.fr.
Claire
C
Chevaleyre
INRA Centre de Val de Loire, UMR-1282 Infectiologie et Santé Publique, F-37380 Nouzilly, France. Electronic address: melo@tours.inra.fr.
Sandrine
S
Melo
INRA Centre de Val de Loire, UMR-1282 Infectiologie et Santé Publique, F-37380 Nouzilly, France. Electronic address: galliano.zanello@utoronto.ca.
Galliano
G
Zanello
INRA Centre de Val de Loire, UMR-1282 Infectiologie et Santé Publique, F-37380 Nouzilly, France. Electronic address: hsalmon@gmail.com.
Henri
H
Salmon
INRA Centre de Val de Loire, UMR-1282 Infectiologie et Santé Publique, F-37380 Nouzilly, France. Electronic address: francois.meurens@usask.ca.
François
F
Meurens
D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D019742:Chemokines, CC / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000276:immunology*; D004927:Escherichia coli Infections / Q000276:immunology*; D020869:Gene Expression Profiling; D006801:Humans; D007113:Immunity, Innate; D007070:Immunoglobulin A / Q000378:metabolism; D007774:Lactation; D042361:Mammary Glands, Human / Q000378:metabolism*; D009092:Mucous Membrane / Q000276:immunology / Q000378:metabolism*; D010950:Plasma Cells / Q000276:immunology*; D011485:Protein Binding; D054447:Receptors, CCR10 / Q000378:metabolism*; D054397:Receptors, CCR3 / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics; D012475:Salmonella / Q000276:immunology*; D012480:Salmonella Infections / Q000276:immunology*; D013552:Swine
D000900:Anti-Bacterial Agents; D019742:Chemokines, CC; D007070:Immunoglobulin A; D054447:Receptors, CCR10; D054397:Receptors, CCR3; D011994:Recombinant Proteins
Antimicrobial; CCL28 receptor; Epithelial; Intestine; Mucosae; Salmonella