Bacillus thuringiensis: a specific gamma-cyclodextrin producer strain.
An anaerobic microbial isolate Bacillus species, designated B. thuringiensis GU-2, was isolated from soil as a specific γ-cyclodextrin (CD) producer strain in alkaline medium under anaerobic conditions. The optimum pH and temperature for bacterial growth and γ-CD production were estimated to be pH 8.5 and 37°C in the presence of 1.0% starch substrate, respectively. A high purity yield >95% of γ-CD from the total CD yield in the reaction mixture was obtained from starch that was supposed to be converted by gamma-cyclodextrin glycotransferase, tentatively named as γ-CGTase. The maximum γ-CGTase activity was estimated at 2.45U/mL under optimized condition. This is the first report demonstrating the generation of a specific γ-cyclodextrin (CD) producer strain by the action of a γ-CGTase under anaerobic conditions.
Department of Pharmaceutical Science, Gachon University, 191 Hambakmoye-ro, Yeonsu-dong, Yeonsu-gu, InCheon 406-799, Republic of Korea.
Bon Geun
BG
Goo
Department of Life Science, Gachon University, 191 Hambakmoye-ro, Yeonsu-dong, Yeonsu-gu, InCheon 406-799, Republic of Korea.
You Jin
YJ
Hwang
Department of Life Science, Gachon University, 191 Hambakmoye-ro, Yeonsu-dong, Yeonsu-gu, InCheon 406-799, Republic of Korea. Electronic address: jkpark@gachon.ac.kr.
Jae Kweon
JK
Park
D001413:Bacillus thuringiensis / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D055162:Biocatalysis; D005285:Fermentation; D005964:Glucosyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D058889:Molecular Typing; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D047408:gamma-Cyclodextrins / Q000378:metabolism*
D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S; D047408:gamma-Cyclodextrins; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase; C023792:gamma-cyclodextrin
Bacillus thuringiensis; Cyclodextrin; Cyclodextrin glycotransferase (CGTase); γ-CD