[Effects of freeze-drying process on polymerized human placenta hemoglobin].
The present study was aimed to investigate the influence of freeze-drying on the quality of polymerized human placenta hemoglobin (PolyPHb). The PolyPHb solution was freeze-drying under suitable conditions. Hemoglobin concentration, methemoglobin (MetHb) content, UV spectrum, Fe3 content, oxygen-carrying capacity, pH, the average molecular weight and its distribution, circular dichroism, oxygen equilibrium curve and other indicators were measured before and after freeze-drying. The appearance, residual water content, rehydration time of the lyophilized product were also evaluated. The results showed that there was no significant difference on all the indicators measured above, which indicated that freeze-drying process had no effect on the physical and chemical properties of PolyPHb, as well as on its biological activity. Therefore, the properties of PolyPHb were stable during this freeze-drying process and could be preserved after such freeze-drying process.
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
Lanzhen
L
Zhao
Institute of Blood Transfusion, Chinese Academy of Medical Sciences, Chengdu 610052, China.
Shen
S
Li
Institute of Tianjin Union Biotechnology Development, Tianjin 300457, China.
Fengjuan
F
Li
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
Jinfeng
J
Wang
Institute of Blood Transfusion, Chinese Academy of Medical Sciences, Chengdu 610052, China.
Chengmin
C
Yang
D001802:Blood Substitutes; D005260:Female; D005612:Freeze Drying / Q000379:methods*; D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D008706:Methemoglobin / Q000032:analysis; D010920:Placenta / Q000737:chemistry*; D058105:Polymerization; D011247:Pregnancy
D001802:Blood Substitutes; D006454:Hemoglobins; D008706:Methemoglobin