Isolation of nuclei from rice tissue grown in suspension culture.
A simple method is described for the isolation of intact nucleic from rice tissue (Oryza sativa L.) grown in suspension culture. The procedure involves incubation of the tissue for 4 h with cellulase and pectinase prior to disruption of the cells. The yield of nucleic is approximately 40% (DNA basis) and the preparations are capable of synthesizing RNA in vitro. The method may be valuable to biochemically oriented research requiring plant nuclei.
Boyce Thompson Institute for Plant Research, Inc., 10701, Yonkers, New York, USA.
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Granados