Antimutagenic and antiherpetic activities of different preparations from Uncaria tomentosa (cat's claw).
Uncaria tomentosa have been used to treat viral diseases such as herpes due to multiple pharmacological effects, but its therapeutic efficacy against this virus have not been reported yet. Thus, in vitro antiherpetic activity of hydroethanolic extract from barks, purified fractions of quinovic acid glycosides and oxindole alkaloids was evaluated by plaque reduction assay, including mechanistic studies (virucidal, attachment and penetration action). Once exposure to physical agents might lead to reactivation of the herpetic infection, antimutagenic effect (pre-, simultaneous and post-treatment protocols) was also evaluated by Comet assay. The antiherpetic activity from the samples under investigation seemed to be associated with the presence of polyphenols or their synergistic effect with oxindole alkaloids or quinovic acid glycosides, once both purified fractions did not present activity when evaluated alone. Inhibition of viral attachment in the host cells was the main mechanism of antiviral activity. Although both purified fractions displayed the lowest antimutagenic activity in pre and simultaneous treatment, they provided a similar effect to that of cat's claw hydroethanolic extract in post-treatment. Given that purified fractions may result in a reduced antiherpetic activity, the use of cat's claw hydroethanolic extract from barks should be prioritized in order to obtain a synergistic effect.
Laboratório de Virologia Aplicada, Universidade Federal de Santa Catarina, Campus Universitário, Trindade, 88040-900 Florianópolis-SC, Brazil.
Thiago
T
Caon
Laboratório de Desenvolvimento Galênico, Departamento de Controle e Produção de Medicamentos, Universidade Federal do Rio Grande do Sul (UFRGS), Faculdade de Farmácia, Santana, 90610-000 Porto Alegre-RS, Brazil.
Samuel
S
Kaiser
Laboratório de Virologia Aplicada, Universidade Federal de Santa Catarina, Campus Universitário, Trindade, 88040-900 Florianópolis-SC, Brazil.
Clarissa
C
Feltrin
Laboratório de Virologia Aplicada, Universidade Federal de Santa Catarina, Campus Universitário, Trindade, 88040-900 Florianópolis-SC, Brazil.
Annelise
A
de Carvalho
Laboratório de Virologia Aplicada, Universidade Federal de Santa Catarina, Campus Universitário, Trindade, 88040-900 Florianópolis-SC, Brazil; Departamento de Análises Clínicas, Universidade Federal de Santa Catarina, Campus Universitário, Trindade, 88040-900 Florianópolis-SC, Brazil.
Thaís Cristine Marques
TC
Sincero
Laboratório de Desenvolvimento Galênico, Departamento de Controle e Produção de Medicamentos, Universidade Federal do Rio Grande do Sul (UFRGS), Faculdade de Farmácia, Santana, 90610-000 Porto Alegre-RS, Brazil.
George González
GG
Ortega
Laboratório de Virologia Aplicada, Universidade Federal de Santa Catarina, Campus Universitário, Trindade, 88040-900 Florianópolis-SC, Brazil; Departamento de Ciências Farmacêuticas, Universidade Federal de Santa Catarina, Campus Universitário, Trindade, 88040-900 Florianópolis-SC, Brazil. Electronic address: claudia.simoes@ufsc.br.
Cláudia Maria Oliveira
CM
Simões
D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D020948:Cat's Claw / Q000737:chemistry*; D002522:Chlorocebus aethiops; D006564:Herpesviridae / Q000187:drug effects* / Q000254:growth & development; D010936:Plant Extracts / Q000494:pharmacology*; D014709:Vero Cells; D010948:Viral Plaque Assay
D016587:Antimutagenic Agents; D000998:Antiviral Agents; D010936:Plant Extracts
Antiherpes activity; Antimutagenic effect; Purified fractions; Uncaria guianensis; Uncaria tomentosa