Anticancer activity of ferulic acid-inorganic nanohybrids synthesized via two different hybridization routes, reconstruction and exfoliation-reassembly.
We have successfully prepared nanohybrids of biofunctional ferulic acid and layered double hydroxide nanomaterials through reconstruction and exfoliation-reassembly routes. From X-ray diffraction and infrared spectroscopy, both nanohybrids were determined to incorporate ferulic acid molecules in anionic form. Microscopic results showed that the nanohybrids had average particle size of 150 nm with plate-like morphology. As the two nanohybridization routes involved crystal disorder and random stacking of layers, the nanohybrids showed slight alteration in z-axis crystallinity and particle size. The zeta potential values of pristine and nanohybrids in deionized water were determined to be positive, while those in cell culture media shifted to negative values. According to the in vitro anticancer activity test on human cervical cancer HeLa cells, it was revealed that nanohybrids showed twice anticancer activity compared with ferulic acid itself. Therefore we could conclude that the nanohybrids of ferulic acid and layered double hydroxide had cellular delivery property of intercalated molecules on cancer cell lines.
Department of Chemistry and Medical Chemistry, College of Science and Technology, Yonsei University, Wonju, Gangwon-do 220-710, Republic of Korea.
Hyoung-Jun
HJ
Kim
Department of Biosystems and Biomaterials Science and Engineering, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
Kitae
K
Ryu
R&I Korea, GBU SC, Solvay, Industry-University Cooperation Building, Ewha Womans University, 150 Bukahyun-ro, Seodaemun-gu, Seoul 120-750, Republic of Korea.
Joo-Hee
JH
Kang
National Institute of Horticultural & Herbal Science (NIHHS) of RDA, Eumseong-gun, Chungcheongbuk-do 369-873, Republic of Korea.
Ae-Jin
AJ
Choi
Department of Biosystems and Biomaterials Science and Engineering, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
Tae-il
TI
Kim
Department of Chemistry and Medical Chemistry, College of Science and Technology, Yonsei University, Wonju, Gangwon-do 220-710, Republic of Korea.
Jae-Min
JM
Oh
D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D003373:Coumaric Acids / Q000737:chemistry* / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D006878:Hydroxides / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D013055:Spectrophotometry, Infrared; D013778:Tetrazolium Salts; D013844:Thiazoles; D014961:X-Ray Diffraction
D000970:Antineoplastic Agents; D003373:Coumaric Acids; D006878:Hydroxides; D013778:Tetrazolium Salts; D013844:Thiazoles; C004999:ferulic acid; C022616:thiazolyl blue