Impedimetric sensor of bacterial toxins based on mixed (Concanavalin A)/polyaniline films.
In this paper, we report the use of Concanavalin A (ConA) and electrosynthesized polyaniline (PANI) thin films for the development of a new electrochemical sensor that allows the specific detection of two bacterial toxins: lipopolysaccharide (LPS) from Escherichia coli and lipoteichoic acid from Staphylococcus aureus. The impedimetric sensor is fabricated by using glutaraldehyde to self-assemble ConA lectin on PANI-modified steel electrodes through covalent binding. ConA acts as a recognition element for bacterial toxins. Electrical impedance spectroscopy (EIS) and scanning electron microscope (SEM) were applied to characterize the assembly process on the modified electrode. The EIS measurements revealed that the resistance charge transfer (RCT) of the electrode/electrolyte interface increases considerably after the ConA lectin interacts with specific carbohydrate moieties present in the molecule of the bacterial toxin. Our results showed that the ConA lectin retained its activity after immobilization on the PANI surface and also the existence of electrochemical impedance response of the bioelectrode which is linear to the extent of the lectin-toxin interaction, with maximum values of RCT for E. coli (14.40 kΩ), and S. aureus (17.80 kΩ). We have observed that electrosynthesized PANI is an excellent support layer for the covalent binding of lectins on the electrode surface. Thus, the recognition system provides an appropriate biomimetic interface for detection of specific constituents of gram-positive and gram-negative bacteria.
Programa de Pós-Graduação em Saúde Humana e Meio Ambiente, UFPE, 55608-680 Vitória de Santo Antão, PE, Brazil.
Jordana S L
JS
da Silva
Departamento de Bioquímica, UFPE, 50670-901 Recife, PE, Brazil.
Maria D L
MD
Oliveira
Departamento de Física, UFPE, 50670-901 Recife, PE, Brazil.
Celso P
CP
de Melo
Programa de Pós-Graduação em Saúde Humana e Meio Ambiente, UFPE, 55608-680 Vitória de Santo Antão, PE, Brazil; Departamento de Bioquímica, UFPE, 50670-901 Recife, PE, Brazil. Electronic address: csrandrade@gmail.com.
Cesar A S
CA
Andrade
D000814:Aniline Compounds / Q000737:chemistry*; D001427:Bacterial Toxins / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D003208:Concanavalin A / Q000737:chemistry*; D058266:Dielectric Spectroscopy / Q000379:methods*; D004926:Escherichia coli / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D013211:Staphylococcus aureus / Q000737:chemistry
D000814:Aniline Compounds; D001427:Bacterial Toxins; C416807:polyaniline; D003208:Concanavalin A
Bacterial toxins; Concanavalin A; Electrical impedance spectroscopy; Polyaniline; Sensor