Relationship of endothelial cell selective adhesion molecule to markers of oxidative stress in type 2 diabetes.
BACKGROUND
Endothelial dysfunction is an important contributor to micro and macrovascular complications of type 2 diabetes (T2D) and is reflected by increased systemic oxidative stress. Endothelial cell selective adhesion molecule (ESAM) influences endothelial function. We aimed to assess, for the first time to our knowledge, the relationship of soluble ESAM to markers of systemic oxidative stress.


METHODS
ESAM, malondialdehyde (MDA) level and catalase activity were determined in 54 T2D patients and 43 controls.


RESULTS
T2D patients had significantly higher ESAM when compared to controls (16.07 ± 5.77 μg/L versus 8.57 ± 5.28 μg/L, p < 0.0001), they also had higher MDA level (3.88 ± 1.50 μmol/L vs. 1.58 ± 0.72 μmol/L, p < 0.0001) and lower catalase activity (3.07 (2.63-3.44) U/mg vs. 8.72 (4.55-10.46) U/mg, p < 0.0001). In T2D patients ESAM was inversely related to catalase activity (r = -0.27, p = 0.04), relationship to MDA level was direct but not significant (r = 0.16, p = 0.24). MDA concentration correlated inversely to catalase activity (r = -0.28, p = 0.04). In multiple regression catalase activity remained significantly correlated to ESAM (p = 0.02) and MDA level was significantly related to glycated hemoglobin (p = 0.01); there was trend towards a positive correlation of MDA level to ESAM (p = 0.08). When patients were divided according to oxidative stress, those with increased oxidative stress (defined as MDA concentration > 2.98 μmol/L and catalase activity < 3.38 U/mg) had higher ESAM than the rest of the patients (17.99 ± 5.02 μg/L vs. 14.29 ± 5.94 μg/L p = 0.01).


CONCLUSIONS
ESAM is higher in T2D than in controls and parallels oxidative stress: ESAM is inversely related to catalase activity and higher ESAM is found in T2D patients with increased oxidative stress.
Department of Nephrology, University of Medicine and Pharmacy "Iuliu Hatieganu" Cluj , Cluj Napoca , Romania.
Ina M
IM
Kacso
Alina R
AR
Potra
Anca
A
Rusu
Diana
D
Moldovan
Crina C
CC
Rusu
Gabriel
G
Kacso
Nicolae D
ND
Hancu
Adriana
A
Muresan
Cosmina I
CI
Bondor
D000368:Aged; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002374:Catalase / Q000097:blood; D015815:Cell Adhesion Molecules / Q000097:blood*; D003430:Cross-Sectional Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000473:pathology; D042783:Endothelial Cells / Q000378:metabolism* / Q000473:pathology; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D018384:Oxidative Stress; D012044:Regression Analysis
D015415:Biomarkers; D015815:Cell Adhesion Molecules; C568779:ESAM protein, human; D008315:Malondialdehyde; D002374:Catalase