Diversity of Campylobacter in retail meat and liver of lambs and goat kids.
The presence, genetic diversity, and antimicrobial susceptibility profile of Campylobacter spp. in retail lamb and goat kid carcasses were assessed. A total of 200 samples consisting of 100 meat and 100 liver surface swabs were collected from 47 lamb and 53 goat kid carcasses at 23 retail markets in Northern Greece, and 125 Campylobacter isolates were recovered from 32 meat surfaces (32%) and 44 liver surfaces (44%). Multiplex polymerase chain reaction and restriction fragment length polymorphism analysis specified Campylobacter coli as the most frequently detected species (59.2%) followed by C. jejuni (40.8%). Pulsed-field gel electrophoresis (PFGE) was applied in order to typify a subset of randomly selected isolates (n=80). SmaI-PFGE successfully clustered the 80 isolates in 38 SmaI-PFGE types, indicating high heterogeneity among the analyzed Campylobacter isolates, and provided data regarding the dissemination of Camplobacter among carcasses stored in the same retail market. Antimicrobial susceptibility profiles of Campylobacter isolates, assessed by the disk-diffusion method, indicated that 31 isolates (24.8%) were multidrug resistant, and the most common profile was the concurrent resistance to tetracycline and streptomycin. Overall, 56.8% of isolates (n=71, multidrug-resistant isolates included) exhibited resistance to at least one antimicrobial (tetracycline 34.4%, quinolones 27.2%, and streptomycin 20.8%). However, all isolates were susceptible to erythromycin and gentamicin. The findings of this study verify the contamination of retail lamb and goat kid carcasses with a heterogeneous population of thermotolerant campylobacters. These data underscore the fact that retail meat and liver of small ruminants could serve as vehicles for consumer contamination with Campylobacter and that further investigation is necessary in order to evaluate the risk imposed by such products within the epidemiology of human campylobacteriosis cases.
1 Laboratory of Hygiene of Foods of Animal Origin, Aristotle University of Thessaloniki (AUTh) , Thessaloniki, Greece .
Thomai
T
Lazou
Chrysostomos
C
Dovas
Kurt
K
Houf
Nikolaos
N
Soultos
Eleni
E
Iossifidou
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques / Q000662:veterinary; D002167:Campylobacter / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002169:Campylobacter Infections / Q000453:epidemiology / Q000382:microbiology* / Q000662:veterinary; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D005516:Food Microbiology; D014644:Genetic Variation; D015511:Goat Diseases / Q000453:epidemiology / Q000382:microbiology*; D006041:Goats; D006115:Greece / Q000453:epidemiology; D006801:Humans; D008099:Liver / Q000382:microbiology; D008460:Meat / Q000382:microbiology*; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial