Safety evaluation of turmeric polysaccharide extract: assessment of mutagenicity and acute oral toxicity.
Curcuma longa Linn. (Zingiberaceae) commonly known as turmeric has long been used for centuries as a spice and household remedy. The present study was carried out to assess the possible mutagenic potential and acute oral toxicity of polysaccharide extract of turmeric rhizome (NR-INF-02) using standard tests. The standard battery of in vitro genotoxicity tests, bacterial reverse mutation test (BRMT), chromosome aberration (CA), and micronucleus (MN) tests were employed to assess the possible mutagenic activity of NR-INF-02 (Turmacin). The results showed no mutagenic effect with NR-INF-02 up to a dose of 5000 µg/mL in BRMT. The results on CA and MN tests revealed the non clastogenic activity of NR-INF-02 in a dose range of 250.36 to 2500 µg/mL with and without metabolic activation (S9). In acute oral toxicity study, NR-INF-02 was found to be safe up to 5 g/kg body weight in Wistar rats. Overall, results indicated that polysaccharide extract of C. longa was found to be genotoxically safe and also exhibited maximum tolerable dose of more than 5 g/kg rat body weight.
R&D Centre, Natural Remedies Private Limited, 5B, Veerasandra Indl. Area, 19th K. M. Stone, Hosur Road, Electronic City Post, Bangalore 560100, Karnataka, India.
Chandrasekaran Chinampudur
CC
Velusami
R&D Centre, Natural Remedies Private Limited, 5B, Veerasandra Indl. Area, 19th K. M. Stone, Hosur Road, Electronic City Post, Bangalore 560100, Karnataka, India.
Srinivasa Rao
SR
Boddapati
R&D Centre, Natural Remedies Private Limited, 5B, Veerasandra Indl. Area, 19th K. M. Stone, Hosur Road, Electronic City Post, Bangalore 560100, Karnataka, India.
Srikanth
S
Hongasandra Srinivasa
R&D Centre, Natural Remedies Private Limited, 5B, Veerasandra Indl. Area, 19th K. M. Stone, Hosur Road, Electronic City Post, Bangalore 560100, Karnataka, India.
Edwin Jothie
EJ
Richard
R&D Centre, Natural Remedies Private Limited, 5B, Veerasandra Indl. Area, 19th K. M. Stone, Hosur Road, Electronic City Post, Bangalore 560100, Karnataka, India.
Joshua Allan
JA
Joseph
R&D Centre, Natural Remedies Private Limited, 5B, Veerasandra Indl. Area, 19th K. M. Stone, Hosur Road, Electronic City Post, Bangalore 560100, Karnataka, India.
Murali
M
Balasubramanian
R&D Centre, Natural Remedies Private Limited, 5B, Veerasandra Indl. Area, 19th K. M. Stone, Hosur Road, Electronic City Post, Bangalore 560100, Karnataka, India.
Amit
A
Agarwal
D000284:Administration, Oral; D000818:Animals; D002869:Chromosome Aberrations / Q000187:drug effects*; D030024:Curcuma / Q000009:adverse effects; D020714:Maximum Tolerated Dose; D009152:Mutagenicity Tests; D010936:Plant Extracts / Q000008:administration & dosage* / Q000009:adverse effects; D011134:Polysaccharides / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012449:Safety
D010936:Plant Extracts; D011134:Polysaccharides; C012596:turmeric extract