Pilot Experimental Study on the Effect of Arginine, Glutamine, and β-Hydroxy β-Methylbutyrate on Secondary Wound Healing.
BACKGROUND
Wound healing is a complex process, dependent on available nutrition substrates. When used together with β-hydroxy β-methylbutyrate, arginine and glutamine have been shown to increase collagen deposition in human subjects. However, there are no experimental investigations on the influence of this amino acid mixture with regard to secondary wound healing. The aim of this study is to investigate the effects of the supplementation of these 3 amino acids on the healing of open wounds in otherwise healthy animals.


METHODS
Twelve rats were divided into control and treatment groups. Two 2-cm × 1-cm full-thickness skin defects were prepared on each subject. The rats in both groups received a diet containing 1.2 g of protein per 100 g of body weight per day. The treatment group, in addition, received 200 mg/kg L-arginine, 200 mg/kg L-glutamine, and 40 mg/kg β-hydroxy β-methylbutyrate every day. Wound sizes were measured every 2 days. On the 10th day, tissue samples were taken for histopathologic evaluation and also for the measurement of hydroxyproline concentrations.


RESULTS
There was no statistically significant difference between mean wound sizes for the 2 groups (P > .05). There was also no statistically significant difference between the groups with regard to histological healing parameters (reepithelialization [P = 1.00], granulation tissue [P = 1.00], collagen accumulation [P = .455], inflammatory cell accumulation [P = .455], angiogenesis [P = .242]) or tissue hydroxyproline concentrations (P = .240).


CONCLUSIONS
Diet supplemented with arginine, glutamine, and β-hydroxy β-methylbutyrate is not beneficial in enhancing secondary healing of open wounds in rats. Further research regarding this topic is warranted.
Department of General Surgery, Ankara Training and Research Hospital, Ankara, Turkey bbozkirli@gmail.com.
Bahadır Osman
BO
Bozkırlı
Department of General Surgery, Ankara Atatürk Training and Research Hospital, Ankara, Turkey.
Rıza H
RH
Gündoğdu
Department of General Surgery, Ankara Atatürk Training and Research Hospital, Ankara, Turkey.
Eren
E
Ersoy
Department of Biomedical Engineering, Boston University, Boston, Massachusetts, USA.
Neşe
N
Lortlar
Department of Biochemistry, Etimesgut Public Health Laboratory, Ankara, Turkey.
Zuhal
Z
Yıldırım
Department of General Surgery, Gemerek State Hospital, Sivas, Turkey.
Hande
H
Temel
Department of General Surgery, Siverek State Hospital, Şanlıurfa, Turkey.
Mehmet
M
Oduncu
Faculty of Medicine, Department of Biostatistics, Hacettepe University, Ankara, Turkey.
Jale
J
Karakaya
D000818:Animals; D001120:Arginine / Q000494:pharmacology*; D003094:Collagen / Q000378:metabolism; D019587:Dietary Supplements; D005973:Glutamine / Q000494:pharmacology*; D006909:Hydroxyproline / Q000378:metabolism; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects* / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D014631:Valerates / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*
D014631:Valerates; D005973:Glutamine; C004961:beta-hydroxyisovaleric acid; D003094:Collagen; D001120:Arginine; D006909:Hydroxyproline
amino acids; arginine; glutamine; immunonutrition; wound healing; β-hydroxy β-methylbutyrate