Evaluation of BMPA, MWY, GVPC and BCYE media for the isolation of Legionella species from respiratory samples.
Culture media performance is a critical factor in the isolation of Legionellae from respiratory samples. We showed that BMPA and MWY media yielded significantly higher isolation rates than GVPC and BCYE media in regard to performance with samples that harbored low Legionella inocula and high contamination levels.
CIRI, International Center for Infectiology Research, Université de Lyon, Lyon, France; INSERM U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France; CNRS, UMR5308, Lyon, France; Hospices Civils de Lyon, Groupement Hospitalier Est, Legionella National Reference Center, East Biology & Pathology Center, 59 Boulevard Pinel, 69677 Bron Cedex, France. Electronic address: ghislaine.descours@univ-lyon1.fr.
G
G
Descours
CIRI, International Center for Infectiology Research, Université de Lyon, Lyon, France; INSERM U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France; CNRS, UMR5308, Lyon, France; Hospices Civils de Lyon, Groupement Hospitalier Est, Legionella National Reference Center, East Biology & Pathology Center, 59 Boulevard Pinel, 69677 Bron Cedex, France; Hospices Civils de Lyon, Edouard Herriot Hospital, Infection Control Unit, 5 Place d'Arsonval, 69437 Lyon Cedex 03, France.
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Cassier
Hospices Civils de Lyon, Groupement Hospitalier Est, Legionella National Reference Center, East Biology & Pathology Center, 59 Boulevard Pinel, 69677 Bron Cedex, France.
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F
Forey
CIRI, International Center for Infectiology Research, Université de Lyon, Lyon, France; INSERM U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France; CNRS, UMR5308, Lyon, France; Hospices Civils de Lyon, Groupement Hospitalier Est, Legionella National Reference Center, East Biology & Pathology Center, 59 Boulevard Pinel, 69677 Bron Cedex, France.
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C
Ginevra
CIRI, International Center for Infectiology Research, Université de Lyon, Lyon, France; INSERM U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France; CNRS, UMR5308, Lyon, France; Hospices Civils de Lyon, Groupement Hospitalier Est, Legionella National Reference Center, East Biology & Pathology Center, 59 Boulevard Pinel, 69677 Bron Cedex, France.
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J
Etienne
CIRI, International Center for Infectiology Research, Université de Lyon, Lyon, France; INSERM U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France; CNRS, UMR5308, Lyon, France; Hospices Civils de Lyon, Groupement Hospitalier Est, Legionella National Reference Center, East Biology & Pathology Center, 59 Boulevard Pinel, 69677 Bron Cedex, France.
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G
Lina
CIRI, International Center for Infectiology Research, Université de Lyon, Lyon, France; INSERM U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France; CNRS, UMR5308, Lyon, France; Hospices Civils de Lyon, Groupement Hospitalier Est, Legionella National Reference Center, East Biology & Pathology Center, 59 Boulevard Pinel, 69677 Bron Cedex, France.
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S
Jarraud
D003470:Culture Media / Q000378:metabolism*; D006801:Humans; D007875:Legionella / Q000302:isolation & purification*; D012137:Respiratory System / Q000382:microbiology*
D003470:Culture Media
BMPA; GVPC; Isolation; Legionella; MWY; Respiratory samples