Correlation of Giardia duodenalis assemblages with clinical and epidemiological data in Cuban children.
Giardia duodenalis is one of the most frequent intestinal parasitic infections in children worldwide. To date, eight main assemblages of G. duodenalis have been described, but only A and B genetic groups are known to infect humans. In Cuba, this parasite has most clinical impact on children. The aim of this investigation was genetic characterization of G. duodenalis isolated from children with giardiasis diagnosed at the Paediatric Hospital "William Soler" between 2010 and 2011, and to compare the genetic results with clinical and epidemiological data. A total of 103 stool samples from 452 children were positive for G. duodenalis and co-infections with other parasites were noted in 5 cases. Assemblage identification was carried out by the amplification of a fragment of the triosephosphate isomerase (tpi) gene. Sub-assemblages of assemblage A (AI and AII) were identified by a nested PCR using the intergenic spacer (IGS) region of ribosomal deoxyribonucleic acid gene as a target. DNA from 90 of 103 (87.4%) samples was successfully amplified by PCR-tpi. The prevalence of assemblages A and B was 40% and 42%, respectively. Infections with both assemblages were reported in 16 cases. No associations between epidemiological information and assemblage was detected, but assemblage B was significantly (P<0.01) more frequently found in children with diarrhea, flatulence or abdominal pain than assemblage A. Sub-assemblage AII accounted for the majority of cases (86.5%).
Department of Parasitology, Tropical Medicine Institute "Pedro Kourí", Autopista Novia del Mediodía Km 6½ e/Autopista Nacional y Carretera Central, La Habana, Cuba. Electronic address: ljerezp@ipk.sld.cu.
Luis Jerez
LJ
Puebla
Department of Parasitology, Tropical Medicine Institute "Pedro Kourí", Autopista Novia del Mediodía Km 6½ e/Autopista Nacional y Carretera Central, La Habana, Cuba.
Fidel A
FA
Núñez
Department of Parasitology, Tropical Medicine Institute "Pedro Kourí", Autopista Novia del Mediodía Km 6½ e/Autopista Nacional y Carretera Central, La Habana, Cuba.
Yenisey Alfonso
YA
Fernández
Department of Parasitology, Tropical Medicine Institute "Pedro Kourí", Autopista Novia del Mediodía Km 6½ e/Autopista Nacional y Carretera Central, La Habana, Cuba.
Jorge
J
Fraga
Department of Parasitology, Tropical Medicine Institute "Pedro Kourí", Autopista Novia del Mediodía Km 6½ e/Autopista Nacional y Carretera Central, La Habana, Cuba.
Lázara Rojas
LR
Rivero
Department of Parasitology, Tropical Medicine Institute "Pedro Kourí", Autopista Novia del Mediodía Km 6½ e/Autopista Nacional y Carretera Central, La Habana, Cuba.
Iraís Atencio
IA
Millán
Paediatric Hospital "William Soler", Avenida 100 y Perla, Altahabana, La Habana, Cuba.
Lucía Ayllón
LA
Valdés
Paediatric Hospital "William Soler", Avenida 100 y Perla, Altahabana, La Habana, Cuba.
Isabel Martínez
IM
Silva
D002648:Child; D002675:Child, Preschool; D060085:Coinfection / Q000453:epidemiology; D003462:Cuba / Q000453:epidemiology; D005243:Feces / Q000469:parasitology; D005260:Female; D017125:Genes, Protozoan; D016829:Giardia lamblia / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D005873:Giardiasis / Q000453:epidemiology* / Q000469:parasitology*; D006801:Humans; D008297:Male; D017422:Sequence Analysis, DNA; D014305:Triose-Phosphate Isomerase / Q000235:genetics
D014305:Triose-Phosphate Isomerase
Assemblages; Children; Cuba; Giardia duodenalis; PCR