Identification of living Legionella pneumophila using species-specific metabolic lipopolysaccharide labeling.
Legionella pneumophila is a pathogenic bacterium involved in regular outbreaks characterized by a relatively high fatality rate and an important societal impact. Frequent monitoring of the presence of this bacterium in environmental water samples is necessary to prevent these epidemic events, but the traditional culture-based detection and identification method requires up to 10 days. Reported herein is a method allowing identification of Legionella pneumophila by metabolic lipopolysaccharide labeling which targets, for the first time, a precursor to monosaccharides that are specifically present within the O-antigen of the bacterium. This new approach allows easy detection of living Legionella pneumophila, while other Legionella species are not labeled.
Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles du CNRS, Avenue de la Terrasse, 91198 Gif-sur-Yvette (France).
Jordi
J
Mas Pons
Audrey
A
Dumont
Grégory
G
Sautejeau
Emilie
E
Fugier
Aurélie
A
Baron
Sam
S
Dukan
Boris
B
Vauzeilles
D057930:Click Chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D016952:Legionella pneumophila / Q000302:isolation & purification* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000378:metabolism; D018613:Microscopy, Confocal; D009005:Monosaccharides / Q000737:chemistry; D019081:O Antigens / Q000737:chemistry / Q000378:metabolism; D012703:Serotyping
D005456:Fluorescent Dyes; D008070:Lipopolysaccharides; D009005:Monosaccharides; D019081:O Antigens
biosynthesis; carbohydrates; click chemistry; imaging agents; synthesis design