Enhanced stabilization of collagen by furfural.
Furfural (2-furancarboxaldehyde), a product derived from plant pentosans, has been investigated for its interaction with collagen. Introduction of furfural during fibril formation enhanced the thermal and mechanical stability of collagen. Collagen films treated with furfural exhibited higher denaturation temperature (Td) (p<0.04) and showed a 3-fold increase in Young's modulus (p<0.04) at higher concentration. Furfural and furfural treated collagen films did not have any cytotoxic effect. Rheological characterization showed an increase in shear stress and shear viscosity with increasing shear rate for treated collagen. Circular dichroism (CD) studies indicated that the furfural did not have any impact on triple helical structure of collagen. Scanning electron microscopy (SEM) of furfural treated collagen exhibited small sized porous structure in comparison with untreated collagen. Thus this study provides an alternate ecologically safe crosslinking agent for improving the stability of collagen for biomedical and industrial applications.
Biomaterials Division, Council of Scientific and Industrial Research, Central Leather Research Institute, Adyar, Chennai 600020, India.
Rachita
R
Lakra
Biomaterials Division, Council of Scientific and Industrial Research, Central Leather Research Institute, Adyar, Chennai 600020, India.
Manikantan Syamala
MS
Kiran
Biophysics Division, Council of Scientific and Industrial Research, Central Leather Research Institute, Adyar, Chennai 600020, India.
Ramamoorthy
R
Usha
Shoe Design and Development Centre, Council of Scientific and Industrial Research, Central Leather Research Institute, Adyar, Chennai 600020, India.
Ranganathan
R
Mohan
Shoe Design and Development Centre, Council of Scientific and Industrial Research, Central Leather Research Institute, Adyar, Chennai 600020, India.
Raja
R
Sundaresan
Biomaterials Division, Council of Scientific and Industrial Research, Central Leather Research Institute, Adyar, Chennai 600020, India. Electronic address: purnasaik.clri@gmail.com.
Purna Sai
PS
Korrapati
D000818:Animals; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D055598:Chemical Phenomena / Q000187:drug effects; D003094:Collagen / Q000737:chemistry*; D005662:Furaldehyde / Q000494:pharmacology* / Q000633:toxicity; D055595:Mechanical Phenomena; D051379:Mice; D041681:NIH 3T3 Cells; D055503:Protein Multimerization / Q000187:drug effects; D055550:Protein Stability / Q000187:drug effects; D017433:Protein Structure, Secondary
D003094:Collagen; D005662:Furaldehyde
Collagen; Collagen stabilization; Furfural; Mechanical properties; Thermal stability