Evaluation of gelatin hydrogel as a potential carrier for cell transportation.
We prepared uncleaved gelatin composed mainly of collagen α-, β-, and γ-chains. Gelation and melting of uncleaved gelatin occurred rapidly with moderate decrease and increase in temperature (23°C-37°C). The viability of cells encapsulated in the gelatin gel was greater than 96% after 7 d at 23°C.
Biotechnology Group, Tokyo Metropolitan Industrial Technology Research Institute, 2-4-10 Aomi, Koto-ku, Tokyo 135-0064, Japan. Electronic address: ohyabu.yoshimi@iri-tokyo.jp.
Yoshimi
Y
Ohyabu
Biotechnology Group, Tokyo Metropolitan Industrial Technology Research Institute, 2-4-10 Aomi, Koto-ku, Tokyo 135-0064, Japan.
Hirosuke
H
Hatayama
Biotechnology Group, Tokyo Metropolitan Industrial Technology Research Institute, 2-4-10 Aomi, Koto-ku, Tokyo 135-0064, Japan.
Shunji
S
Yunoki
D000818:Animals; D002470:Cell Survival; D005780:Gelatin / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D011309:Preservation, Biological; D013696:Temperature; D014186:Transportation
D020100:Hydrogels; D005780:Gelatin
Cell carrier; Collagen; Gel; Gelatin; Renaturation; Transportation