Initiation of the flexirubin biosynthesis in Chitinophaga pinensis.
Bacteria from the Bacteroidetes phylum are known producers of the chemotaxonomic relevant flexirubins. These orange pigments comprise a non-isoprenoid aryl-polyene carboxylic acid esterified with a dialkylresorcinol. Herein, we report a gene cluster from Chitinophaga pinensis encoding the biosynthesis of the polyene moiety and the biochemical characterization of a tyrosine ammonia-lyase and a 4-coumarate-CoA ligase responsible for the initiation of the polyene biosynthesis. Additionally, the flexirubin of C. pinensis was characterized by a combination of feeding experiments, high-performance liquid chromatography tandem mass spectrometry and matrix-assisted laser desorption/ionization mass spectrometry.
Merck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Max-von-Laue-Str. 9, Frankfurt am Main, 60438, Germany.
Tim A
TA
Schöner
Sebastian W
SW
Fuchs
Christian
C
Schönau
Helge B
HB
Bode
D000642:Ammonia-Lyases / Q000235:genetics / Q000378:metabolism; D041963:Bacteroidetes / Q000235:genetics* / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000235:genetics*; D002851:Chromatography, High Pressure Liquid; D003066:Coenzyme A Ligases / Q000235:genetics / Q000378:metabolism; D005810:Multigene Family; D011090:Polyenes / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
D011090:Polyenes; C017313:flexirubins; D000642:Ammonia-Lyases; C023208:L-tyrosine ammonia-lyase; D003066:Coenzyme A Ligases; C019943:4-coumarate-CoA ligase