Metabolic changes in serum steroids induced by total-body irradiation of female C57B/6 mice.
The short- and long-term effects of a single exposure to gamma radiation on steroid metabolism were investigated in mice. Gas chromatography-mass spectrometry was used to generate quantitative profiles of serum steroid levels in mice that had undergone total-body irradiation (TBI) at doses of 0Gy, 1Gy, and 4Gy. Following TBI, serum samples were collected at the pre-dose time point and 1, 3, 6, and 9 months after TBI. Serum levels of progestins, progesterone, 5β-DHP, 5α-DHP, and 20α-DHP showed a significant down-regulation following short-term exposure to 4Gy, with the exception of 20α-DHP, which was significantly decreased at each of the time points measured. The corticosteroids 5α-THDOC and 5α-DHB were significantly elevated at each of the time points measured after exposure to either 1 or 4Gy. Among the sterols, 24S-OH-cholestoerol showed a dose-related elevation after irradiation that reached significance in the high dose group at the 6- and 9-month time points.
Future Convergence Research Division, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea.
Ju-Yeon
JY
Moon
Radiation Biotechnology Research Division, Korea Atomic Energy Research Institute, Jeonbuk 580-185, Republic of Korea.
Hee-June
HJ
Shin
Future Convergence Research Division, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea.
Hyun-Hwa
HH
Son
Future Convergence Research Division, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea.
Jeongae
J
Lee
Radiation Biotechnology Research Division, Korea Atomic Energy Research Institute, Jeonbuk 580-185, Republic of Korea.
Uhee
U
Jung
Radiation Biotechnology Research Division, Korea Atomic Energy Research Institute, Jeonbuk 580-185, Republic of Korea.
Sung-Kee
SK
Jo
Division of Mass Spectrometry Research, Korea Basic Science Institute, Chungcheongbuk-do 363-883, Republic of Korea.
Hyun Sik
HS
Kim
Division of Mass Spectrometry Research, Korea Basic Science Institute, Chungcheongbuk-do 363-883, Republic of Korea.
Kyung-Hoon
KH
Kwon
Division of Mass Spectrometry Research, Korea Basic Science Institute, Chungcheongbuk-do 363-883, Republic of Korea.
Kyu Hwan
KH
Park
Future Convergence Research Division, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea.
Bong Chul
BC
Chung
Future Convergence Research Division, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea. Electronic address: mh_choi@kist.re.kr.
Man Ho
MH
Choi
D015089:20-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000818:Animals; D015536:Down-Regulation / Q000528:radiation effects*; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D011372:Progestins / Q000097:blood*; D013256:Steroids / Q000097:blood*; D015430:Weight Gain / Q000528:radiation effects; D014916:Whole-Body Irradiation
D011372:Progestins; D013256:Steroids; D015089:20-Hydroxysteroid Dehydrogenases
Aging; Gas chromatography–mass spectrometry; Steroid metabolism; Steroid signatures; Total-body irradiation