Near full-length genome identification of a novel HIV-1 recombinant form (CRF01_AE/B'/C) among heterosexuals in Jilin, China.
Recombinant forms contribute significantly to the genetic diversity of HIV-1. Here we report a novel HIV-1 recombinant form (CRF01_AE/B'/C) detected from a comprehensive HIV-1 molecular epidemiologic study among heterosexuals in Jilin province of northeastern China. Recombinant analyses of the near full-length genome (NFLG) of the novel HIV-1 recombinant isolate (JL.RF01) showed that the backbone of the genome was CRF01_AE, and three insertions of subtype B' (242, 370, and 233 bp) and C (1142, 230, and 271 bp), respectively, were inserted along the genome. Phylogenetic analyses revealed that the novel HIV-1 recombinant form (CRF01_AE/B'/C) more likely originated from Thailand subtype B' and CRF01_AE and India subtype C. We report a unique mosaic structure that is distinct to HIV-1 CRF01_AE/B'/C recombinant viruses reported to date. The emergence of this novel recombinant form (CRF01_AE/B'/C) suggests the increasing significance of heterosexual transmission contributing to the complexity of the HIV-1 epidemic in northeastern China.
1 State Key Laboratory for Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention , Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Beijing, China .
Xingguang
X
Li
Chuanyi
C
Ning
Yanli
Y
Chen
Yi
Y
Feng
Min
M
Wei
Hui
H
Xing
Yiming
Y
Shao
D001483:Base Sequence; D002681:China / Q000453:epidemiology; D014644:Genetic Variation; D016679:Genome, Viral / Q000235:genetics*; D015658:HIV Infections / Q000453:epidemiology* / Q000635:transmission / Q000821:virology; D015497:HIV-1 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D020010:Heterosexuality; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D011995:Recombination, Genetic; D017423:Sequence Analysis, RNA
D012367:RNA, Viral