Downmodulation of peripheral MOG-specific immunity by pVAXhsp65 treatment during EAE does not reach the CNS.
Most of the therapeutic strategies to control multiple sclerosis are directed to immune modulation and inflammation control. As heat shock proteins are able to induce immunoregulatory T cells, we investigated the therapeutic effect of a genetic vaccine containing the mycobacterial hsp65 gene on experimental autoimmune encephalomyelitis (EAE). Although pVAXhsp65 was immunogenic for mice with EAE and downmodulated specific cytokine induction by MOG, therapy was not able to decrease clinical severity nor to modify immunologic parameters in the CNS. These results indicate that hsp65, administered as a DNA vaccine, was not therapeutic for EAE.
Department of Microbiology and Immunology, Biosciences Institute, Univ. Estadual Paulista (UNESP), Botucatu, São Paulo, Brazil.
Sofia Fernanda Gonçalves
SF
Zorzella-Pezavento
Department of Microbiology and Immunology, Biosciences Institute, Univ. Estadual Paulista (UNESP), Botucatu, São Paulo, Brazil.
Fernanda
F
Chiuso-Minicucci
Department of Microbiology and Immunology, Biosciences Institute, Univ. Estadual Paulista (UNESP), Botucatu, São Paulo, Brazil.
Thais Graziela Donegá
TG
França
Department of Microbiology and Immunology, Biosciences Institute, Univ. Estadual Paulista (UNESP), Botucatu, São Paulo, Brazil.
Larissa Lumi Watanabe
LL
Ishikawa
Department of Microbiology and Immunology, Biosciences Institute, Univ. Estadual Paulista (UNESP), Botucatu, São Paulo, Brazil.
Larissa Camargo
LC
da Rosa
Department of Microbiology and Immunology, Biosciences Institute, Univ. Estadual Paulista (UNESP), Botucatu, São Paulo, Brazil.
Priscila Maria
PM
Colavite
Laboratório de Citometria de Fluxo - Fundação Dr. Amaral Carvalho, Jaú, São Paulo, Brazil.
Camila
C
Marques
Laboratório de Citometria de Fluxo - Fundação Dr. Amaral Carvalho, Jaú, São Paulo, Brazil.
Maura Rosane Valerio
MR
Ikoma
Department of Biochemistry and Immunology, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil.
Célio Lopes
CL
Silva
Department of Microbiology and Immunology, Biosciences Institute, Univ. Estadual Paulista (UNESP), Botucatu, São Paulo, Brazil. Electronic address: sartori@ibb.unesp.br.
Alexandrina
A
Sartori
D000818:Animals; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000473:pathology* / Q000517:prevention & control; D005260:Female; D006360:Heat-Shock Proteins / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D063308:Myelin-Oligodendrocyte Glycoprotein / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology / Q000494:pharmacology*; D018834:Chaperonin 60; D024101:Mitochondrial Proteins
D016207:Cytokines; D006360:Heat-Shock Proteins; D063308:Myelin-Oligodendrocyte Glycoprotein; D019444:Vaccines, DNA; C571327:Mog protein, mouse; C557066:Hspd1 protein, mouse; D018834:Chaperonin 60; D024101:Mitochondrial Proteins
CD4+CD25+Foxp3+ T cells; DNA vaccine; Experimental autoimmune encephalomyelitis; hsp65