Human ethmoid sinus mucosa: a promising novel tissue source of mesenchymal progenitor cells.
BACKGROUND
The identification of new progenitor cell sources is important for cell-based tissue engineering strategies, understanding regional tissue regeneration, and modulating local microenvironments and immune response. However, there are no reports that describe the identification and isolation of mesenchymal progenitor cells (MPCs) from paranasal sinus mucosa, and compare the properties of MPCs between tissue sources within the sinonasal cavity. We report here the identification of MPCs in the maxillary sinus (MS) and ethmoid sinus (ES). Furthermore, we contrast these MPCs in the same individuals with MPCs from two additional head and neck tissue sources of the inferior turbinate (IT) and tonsil (T).


METHODS
These four MPC sources were exhaustively compared for morphology, colony-forming potential, proliferation capability, immunophenotype, multilineage differentiation potential, and ability to produce soluble factors.


RESULTS
MS-, ES, IT-, and T-MPCs showed similar morphologies and surface phenotypes, as well as adipogenic, osteogenic, and chondrogenic differentiation capacity by immunohistochemistry and qRT-PCR for defined lineage-specific genes. However, we noted that the colony-forming potential and proliferation capability of ES-MPCs were distinctly higher than other MPCs. All MPCs constitutively, or upon stimulation, secrete large amounts of IL-6, IL-8, IL-10, IFN-γ, and TGF-β. After stimulation with TNF-α and IFN-γ, ES-MPCs notably demonstrated significantly higher secretion of IL-6 and IL-10 than other MPCs.


CONCLUSIONS
ES-MPCs may be a uniquely promising source of MPCs due to their high proliferation ability and superior capacity toward secretion of immunomodulatory cytokines.
Kyu-Sup
KS
Cho
Hee-Young
HY
Park
Hwan-Jung
HJ
Roh
Dawn T
DT
Bravo
Peter H
PH
Hwang
Jayakar V
JV
Nayak
D000328:Adult; D002454:Cell Differentiation; D049109:Cell Proliferation; D005005:Ethmoid Sinus / Q000166:cytology*; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007378:Interleukins / Q000235:genetics / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism / Q000502:physiology; D008875:Middle Aged; D009297:Nasal Mucosa / Q000166:cytology*
D007378:Interleukins