Structure-function relationships and supramolecular organization of the EGFR (epidermal growth factor receptor) on the cell surface.
Dimerization and higher-order oligomerization are believed to play an important role in the activation of the EGFR (epidermal growth factor receptor). Understanding of the process has been limited by the lack of availability of suitable methods for the measurement in cells of distances in the range 10-100 nm, too short for imaging methods and too long for spectroscopic methods such as FRET. In the present article, we review the current state of our knowledge of EGFR oligomerization, and describe results from a new single-molecule localization method that has allowed the quantitative characterization of the distribution of EGFR-EGFR distances in cells. Recent data suggest the involvement of cortical actin in regulating the formation of EGFR complexes.
*Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire OX11 0FA, U.K.
Sarah R
SR
Needham
*Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire OX11 0FA, U.K.
Laura C
LC
Zanetti-Domingues
*Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire OX11 0FA, U.K.
Michael
M
Hirsch
*Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire OX11 0FA, U.K.
Daniel J
DJ
Rolfe
*Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire OX11 0FA, U.K.
Christopher J
CJ
Tynan
*Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire OX11 0FA, U.K.
Selene K
SK
Roberts
*Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire OX11 0FA, U.K.
Marisa L
ML
Martin-Fernandez
*Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire OX11 0FA, U.K.
David T
DT
Clarke
D002462:Cell Membrane / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000502:physiology; D066246:ErbB Receptors / Q000737:chemistry / Q000502:physiology*; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D008958:Models, Molecular; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary
D004815:Epidermal Growth Factor; C512478:EGFR protein, human; D066246:ErbB Receptors