miR-34a targets the inhibin beta B gene, promoting granulosa cell apoptosis in the porcine ovary.
During ovarian follicular growth and development, only a few follicles actually ovulate. Recently, it was found that follicular atresia is triggered by granulosa cell apoptosis, but the molecular mechanism of follicular atresia was not understood. Using flow cytometry, we found that miR-34a promotes granulosa cell apoptosis in pig ovarian follicles. In addition, inhibin beta B was found to be a miR-34a target gene, based on luciferase reporter assays, quantitative RT-PCR and Western blotting. Taken together, our data indicate that miR-34a plays an important role in granulosa cell apoptosis by targeting the INHBB gene in the porcine ovary.
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
F
F
Tu
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Z X
ZX
Pan
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Y
Y
Yao
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
H L
HL
Liu
Animal Husbandry and Veterinary Institute, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, China.
S R
SR
Liu
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China zxie@njau.edu.cn.
Z
Z
Xie
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Q F
QF
Li
D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D005260:Female; D005434:Flow Cytometry; D006107:Granulosa Cells / Q000473:pathology; D028322:Inhibin-beta Subunits / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics*; D006080:Ovarian Follicle / Q000254:growth & development / Q000378:metabolism; D010053:Ovary / Q000378:metabolism*; D034421:Sus scrofa; D013552:Swine
D035683:MicroRNAs; D028322:Inhibin-beta Subunits