Effect of Lactobacillus plantarum C014 on innate immune response and disease resistance against Aeromonas hydrophila in hybrid catfish.
A bacterial strain isolated from intestines of hybrid catfish (Clarias gariepinus Male × Clarias macrocephalus Female) exhibited an in vitro inhibitory effect on a fish pathogen, Aeromonas hydrophila TISTR 1321. By using the 16S rDNA sequence analysis, it was identified as Lactobacillus plantarum C014. To examine whether L. plantarum C014 had potential for use as an immunostimulant and biocontrol agent in hybrid catfish, the fish diet supplemented with L. plantarum C014 (10(7) CFU/g diet) was prepared and used for the in vivo investigation of its effect on innate immune response and disease resistance of hybrid catfish. Two innate immune response parameters, phagocytic activity of blood leukocytes and plasma lysozyme activity, were significantly enhanced in the treated fish after 45 days of feeding. Feeding the fish with the L. plantarum C014 supplemented diet for 45 days before challenging them with A. hydrophila at the dose of LD50 could reduce the mortality rate of the fish from 50% (in control group) to 0% (in treated group). Based on its origin and beneficial effect on innate immune response and disease resistance, L. plantarum C014 may be a potential candidate for use as a natural and safe immunostimulant and biocontrol agent in hybrid catfish.
Department of Biological Science, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani 34190, Thailand.
Sureerat
S
Butprom
Department of Biological Science, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani 34190, Thailand.
Parichat
P
Phumkhachorn
Department of Biological Science, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani 34190, Thailand.
Pongsak
P
Rattanachaikunsopon
D016980:Aeromonas hydrophila / Q000276:immunology*; D000704:Analysis of Variance; D000818:Animals; D001483:Base Sequence; D002397:Catfishes; D017931:DNA Primers / Q000235:genetics; D019587:Dietary Supplements; D060467:Disease Resistance / Q000276:immunology*; D005393:Fish Diseases / Q000235:genetics / Q000276:immunology* / Q000382:microbiology*; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000662:veterinary*; D006824:Hybridization, Genetic / Q000276:immunology; D007113:Immunity, Innate / Q000276:immunology*; D048191:Lactiplantibacillus plantarum / Q000737:chemistry* / Q000235:genetics; D007928:Lethal Dose 50; D008969:Molecular Sequence Data; D009113:Muramidase / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects / Q000276:immunology; D019936:Probiotics / Q000494:pharmacology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000662:veterinary
D017931:DNA Primers; D012336:RNA, Ribosomal, 16S; D009113:Muramidase