The effect of brain-derived neurotrophic factor on periodontal furcation defects.
This study aimed to observe the regenerative effect of brain-derived neurotrophic factor (BDNF) in a non-human primate furcation defect model. Class II furcation defects were created in the first and second molars of 8 non-human primates to simulate a clinical situation. The defect was filled with either, Group A: BDNF (500 µg/ml) in high-molecular weight-hyaluronic acid (HMW-HA), Group B: BDNF (50 µg/ml) in HMW-HA, Group C: HMW-HA acid only, Group D: empty defect, or Group E: BDNF (500 µg/ml) in saline. The healing status for all groups was observed at different time-points with micro computed tomography. The animals were euthanized after 11 weeks, and the tooth-bone specimens were subjected to histologic processing. The results showed that all groups seemed to successfully regenerate the alveolar buccal bone, however, only Group A regenerated the entire periodontal tissue, i.e., alveolar bone, cementum and periodontal ligament. It is suggested that the use of BDNF in combination with a scaffold such as the hyaluronic acid in periodontal furcation defects may be an effective treatment option.
Department of Prosthodontics, Faculty of Odontology, Malmo University, Malmö, Sweden.
Ryo
R
Jimbo
Department of Biomaterials and Biomimetics, New York University College of Dentistry, New York, New York, United States of America.
Nick
N
Tovar
Department of Epidemiology and Health Promotion, New York University, New York, United States of America.
Malvin N
MN
Janal
Department of Biomaterials and Biomimetics, New York University College of Dentistry, New York, New York, United States of America.
Ramy
R
Mousa
Department of Dentistry, UNIGRANRIO, Duque de Caxias, Rio de Janeiro, Brazil ; Department of Prosthodontics, Integrated Center for Research, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
Charles
C
Marin
Department of Biomaterials and Biomimetics, New York University College of Dentistry, New York, New York, United States of America.
Daniel
D
Yoo
Department of Biomaterials and Biomimetics, New York University College of Dentistry, New York, New York, United States of America.
Hellen S
HS
Teixeira
Department of Biomaterials and Biomimetics, New York University College of Dentistry, New York, New York, United States of America.
Rodolfo B
RB
Anchieta
Department of Prosthodontics, Integrated Center for Research, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
Estevam A
EA
Bonfante
Department of Dentistry, UNIGRANRIO, Duque de Caxias, Rio de Janeiro, Brazil ; Department of Prosthodontics, Integrated Center for Research, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
Akihiro
A
Konishi
Department of Periodontal Medicine, Division of Applied Life Sciences, Hiroshima University Institute of Biomedical & Health Sciences, Minami-ku, Hiroshima, Japan.
Katsuhiro
K
Takeda
Department of Periodontal Medicine, Division of Applied Life Sciences, Hiroshima University Institute of Biomedical & Health Sciences, Minami-ku, Hiroshima, Japan.
Hidemi
H
Kurihara
Department of Biomaterials and Biomimetics, New York University College of Dentistry, New York, New York, United States of America.
Paulo G
PG
Coelho
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology* / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D017823:Furcation Defects / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D006820:Hyaluronic Acid / Q000737:chemistry / Q000494:pharmacology; D008252:Macaca fascicularis; D008970:Molecular Weight; D010519:Periodontium / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D012038:Regeneration / Q000187:drug effects
D019208:Brain-Derived Neurotrophic Factor; D006820:Hyaluronic Acid