Enantioselective disruption of the endocrine system by Cis-Bifenthrin in the male mice.
Bifenthrin (BF), as a chiral pyrethroid, is widely used to control field and household pests in China. At present, the commercial BF is a mixed compound containing cis isomers (cis-BF) including two enantiomers of 1R-cis-BF and 1S-cis-BF. In the present study, the two individual cis-BF enantiomers were separated by a preparative supercritical fluid chromatography. Then, four week-old adolescent male ICR mice were orally administered 1R-cis-BF and 1S-cis-BF separately daily for 3 weeks at doses of 0, 7.5 and 15 mg/kg/day, respectively. Results showed that the transcription status of some genes involved in cholesterol synthesis and transport as well as testosterone (T) synthesis in the testes were influenced by cis-BF enantiomers. Especially, we observed that the transcription status of key genes on the pathway of T synthesis including cytochrome P450 cholesterol side-chain cleavage enzyme (P450scc) and cytochrome P450 17α-hydroxysteroid dehydrogenase (P45017α)) were selectively altered in the testis of mice when treated with 1S-cis-BF, suggesting that it is the possible reason to explain why the lower serum T concentration in 1S-cis-BF treated group. Taken together, it concluded that both of the cis-BF enantiomers have the endocrine disruption activities, while 1S-cis-BF was higher than 1R-cis-BF in mice when exposed during the puberty. The data was helpful to understand the toxicity of cis-BF in mammals under enantiomeric level.
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, China.
Yuanxiang
Y
Jin
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, China.
Jiangcong
J
Wang
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, China.
Xiuhong
X
Pan
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, China.
Wenyu
W
Miao
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, China.
Xiaojian
X
Lin
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, China.
Linggang
L
Wang
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, China.
Zhengwei
Z
Fu
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000378:metabolism; D052244:Endocrine Disruptors / Q000633:toxicity*; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation / Q000187:drug effects; D006913:Hydroxysteroid Dehydrogenases / Q000378:metabolism; D007306:Insecticides / Q000737:chemistry / Q000633:toxicity*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011722:Pyrethrins / Q000633:toxicity*; D013237:Stereoisomerism; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D013739:Testosterone / Q000097:blood
D052244:Endocrine Disruptors; D007306:Insecticides; D011722:Pyrethrins; D013739:Testosterone; C099952:bifenthrin; D006913:Hydroxysteroid Dehydrogenases; C035507:3(17)-hydroxysteroid dehydrogenase; D002786:Cholesterol Side-Chain Cleavage Enzyme
bifenthrin; enantioselectivity; endocrine disruption; gene expression