Enzymatic conversion of flavonoids using bacterial chalcone isomerase and enoate reductase.
Flavonoids are a large group of plant secondary metabolites with a variety of biological properties and are therefore of interest to many scientists, as they can lead to industrially interesting intermediates. The anaerobic gut bacterium Eubacterium ramulus can catabolize flavonoids, but until now, the pathway has not been experimentally confirmed. In the present work, a chalcone isomerase (CHI) and an enoate reductase (ERED) could be identified through whole genome sequencing and gene motif search. These two enzymes were successfully cloned and expressed in Escherichia coli in their active form, even under aerobic conditions. The catabolic pathway of E. ramulus was confirmed by biotransformations of flavanones into dihydrochalcones. The engineered E. coli strain that expresses both enzymes was used for the conversion of several flavanones, underlining the applicability of this biocatalytic cascade reaction.
Institute of Biochemistry, Department of Biotechnology & Enzyme Catalysis, Greifswald University, Felix-Hausdorff-Strasse 4, 17487 Greifswald (Germany) http://biotech.uni-greifswald.de.
Mechthild
M
Gall
Maren
M
Thomsen
Christin
C
Peters
Ioannis V
IV
Pavlidis
Patrick
P
Jonczyk
Philipp P
PP
Grünert
Sascha
S
Beutel
Thomas
T
Scheper
Egon
E
Gross
Michael
M
Backes
Torsten
T
Geissler
Jakob P
JP
Ley
Jens-Michael
JM
Hilmer
Gerhard
G
Krammer
Gottfried J
GJ
Palm
Winfried
W
Hinrichs
Uwe T
UT
Bornscheuer
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D055162:Biocatalysis; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000378:metabolism; D005051:Eubacterium / Q000201:enzymology* / Q000235:genetics; D044950:Flavanones / Q000737:chemistry / Q000378:metabolism; D005419:Flavonoids / Q000737:chemistry / Q000378:metabolism*; D019753:Intramolecular Lyases / Q000235:genetics / Q000378:metabolism*; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D020836:Protein Structure, Quaternary; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D044950:Flavanones; D005419:Flavonoids; D011994:Recombinant Proteins; D010088:Oxidoreductases; D019753:Intramolecular Lyases; C019865:chalcone isomerase
Eubacterium ramulus; chalcone isomerase; enoate reductase; enzyme biocatalysis; flavonoids