[The preparation and bioactivity assessment of gene recombinant fibrin-binding brain derived neurotrophic factor].
Using the E. coli, we fabricated the gene reconstructed brain derived neurotrophic factor with a fibrin binding domain (FBD-BDNF). We then tested the neurotrophic bioactivity and fibrin-binding ability of the FBD-BDNF. The E. coli was used to express the recombinant protein. The inclusion body was purified with column chromatography and renaturated to construct the right 3D formation. In this study, we successfully fabricated the FBD-BDNF and tested the binding ability and neurotrophic activity. The results demonstrated that FBD-BDNF had special binding ability of fibrin and significant neurotrophic activity for DRG cells. FBD-BDNF could have a promising application prospect in nerve tissue engineering.
National Institute for Food and Drug Control, Beijing 100050, China.
Qianqian
Q
Han
National Institute for Food and Drug Control, Beijing 100050, China.
Xiaoming
X
Feng
National Institute for Food and Drug Control, Beijing 100050, China.
Chunren
C
Wang
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China.
Jianwu
J
Dai
National Institute for Food and Drug Control, Beijing 100050, China.
Zhaopeng
Z
Yang
D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005337:Fibrin / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics
D019208:Brain-Derived Neurotrophic Factor; D011993:Recombinant Fusion Proteins; D005337:Fibrin