Genetically engineered bacteriophage delivers a tumor necrosis factor alpha antagonist coating on neural electrodes.
This paper reports a novel approach for the formation of anti-inflammatory surface coating on a neural electrode. The surface coating is realized using a recombinant f88 filamentous bacteriophage, which displays a short platinum binding motif and a tumor necrosis factor alpha antagonist (TNF-α antagonist) on p3 and p8 proteins, respectively. The recombinant bacteriophages are immobilized on the platinum surface by a simple dip coating process. The selective and stable immobilization of bacteriophages on a platinum electrode is confirmed by quartz crystal microbalance with dissipation monitoring, atomic force microscope and fluorescence microscope. From the in vitro cell viability test, the inflammatory cytokine (TNF-α) induced cell death was prevented by presenting recombinant bacteriophage coating, albeit with no significant cytotoxic effect. It is also observed that the bacteriophage coating does not have critical effects on the electrochemical properties such as impedance and charge storage capacities. Thus, this approach demonstrates a promising anti-apoptotic as well as anti-inflammatory surface coating for neural implant applications.
Laboratory of Nanomedicine, Korea Institute of Science and Technology Europe (KIST-Europe) Forschungsgesellschaft mbH, Campus E 7 1, Saarbruecken, Germany.
Young Jun
YJ
Kim
Young-Hyun
YH
Jin
Georgette B
GB
Salieb-Beugelaar
Chang-Hoon
CH
Nam
Thomas
T
Stieglitz
D020816:Amino Acid Motifs; D000893:Anti-Inflammatory Agents / Q000737:chemistry; D017209:Apoptosis; D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D045744:Cell Line, Tumor; D002470:Cell Survival; D004247:DNA / Q000737:chemistry; D016503:Drug Delivery Systems; D004548:Elasticity; D004566:Electrodes; D005818:Genetic Engineering / Q000379:methods*; D006801:Humans; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D010984:Platinum / Q000737:chemistry; D013499:Surface Properties; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000737:chemistry; D014783:Viscosity
D000893:Anti-Inflammatory Agents; D010455:Peptides; D014409:Tumor Necrosis Factor-alpha; D010984:Platinum; D004247:DNA