0.5 gigapixel microscopy using a flatbed scanner.
The capability to perform high-resolution, wide field-of-view (FOV) microscopy imaging is highly sought after in biomedical applications. In this paper, we report a wide FOV microscopy system that uses a closed-circuit-television (CCTV) lens for image relay and a flatbed scanner for data acquisition. We show that such an imaging system is capable of capturing a 10 mm × 7.5 mm FOV image with 0.78 µm resolution, resulting in more than 0.5 billion pixels across the entire image. The resolution and field curve of the proposed system were characterized by imaging a USAF resolution target and a hole-array target. To demonstrate its application, 0.5 gigapixel images of histology slides were acquired using this system.
Electrical Engineering, California Institute of Technology, Pasadena, CA, 91125, USA ; Presently at Biomedical Engineering & Electrical Engineering, University of Connecticut, Storss, CT, 06269 USA ; These authors contributed equally to this work.
Guoan
G
Zheng
Electrical Engineering, California Institute of Technology, Pasadena, CA, 91125, USA ; These authors contributed equally to this work.
Xiaoze
X
Ou
Electrical Engineering, California Institute of Technology, Pasadena, CA, 91125, USA.
Changhuei
C
Yang
(120.4820) Optical systems; (170.0180) Microscopy; (170.4730) Optical pathology