Ligand-dependent stabilization of androgen receptor in a novel mouse ST38c Sertoli cell line.
Mature Sertoli cells (SC) are critical mediators of androgen regulation of spermatogenesis, via the androgen receptor (AR) signaling. Available immortalized SC lines loose AR expression or androgen responsiveness, hampering the study of endogenous AR regulation in SC. We have established and characterized a novel clonal mouse immortalized SC line, ST38c. These cells express some SC specific genes (sox9, wt1, tjp1, clu, abp, inhbb), but not fshr, yet more importantly, maintain substantial expression of endogenous AR as determined by PCR, immunocytochemistry, testosterone binding assays and Western blots. Microarrays allowed identification of some (146) but not all (rhox5, spinlw1), androgen-dependent, SC expressed target genes. Quantitative Real-Time PCR validated regulation of five up-regulated and two down-regulated genes. We show that AR undergoes androgen-dependent transcriptional activation as well as agonist-dependent posttranslational stabilization in ST38c cells. This cell line constitutes a useful experimental tool for future investigations on the molecular and cellular mechanisms of androgen receptor signaling in SC function.
INSERM U693 and Univ Paris-Sud 11, Faculté de Médecine Paris-Sud, UMR-S693, Le Kremlin Bicêtre F-94276, France; «Carol Davila» University of Medicine and Pharmacy, Bucharest, Romania.
Lavinia
L
Vija
INSERM U693 and Univ Paris-Sud 11, Faculté de Médecine Paris-Sud, UMR-S693, Le Kremlin Bicêtre F-94276, France.
Kahina
K
Boukari
INSERM U693 and Univ Paris-Sud 11, Faculté de Médecine Paris-Sud, UMR-S693, Le Kremlin Bicêtre F-94276, France.
Hugues
H
Loosfelt
INSERM U693 and Univ Paris-Sud 11, Faculté de Médecine Paris-Sud, UMR-S693, Le Kremlin Bicêtre F-94276, France.
Geri
G
Meduri
INSERM U693 and Univ Paris-Sud 11, Faculté de Médecine Paris-Sud, UMR-S693, Le Kremlin Bicêtre F-94276, France.
Say
S
Viengchareun
INSERM U693 and Univ Paris-Sud 11, Faculté de Médecine Paris-Sud, UMR-S693, Le Kremlin Bicêtre F-94276, France; Assistance Publique-Hôpitaux de Paris, Hôpital de Bicêtre, Service d'Endocrinologie et Maladies de la Reproduction, Le Kremlin Bicêtre F-94275, France.
Nadine
N
Binart
INSERM U693 and Univ Paris-Sud 11, Faculté de Médecine Paris-Sud, UMR-S693, Le Kremlin Bicêtre F-94276, France; Assistance Publique-Hôpitaux de Paris, Hôpital de Bicêtre, Service d'Endocrinologie et Maladies de la Reproduction, Le Kremlin Bicêtre F-94275, France.
Jacques
J
Young
INSERM U693 and Univ Paris-Sud 11, Faculté de Médecine Paris-Sud, UMR-S693, Le Kremlin Bicêtre F-94276, France; Assistance Publique-Hôpitaux de Paris, Hôpital de Bicêtre, Service d'Endocrinologie et Maladies de la Reproduction, Le Kremlin Bicêtre F-94275, France. Electronic address: marc.lombes@u-psud.fr.
Marc
M
Lombès
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002461:Cell Line, Transformed / Q000166:cytology / Q000378:metabolism*; D018703:Founder Effect; D015870:Gene Expression; D005786:Gene Expression Regulation; D008024:Ligands; D008297:Male; D051379:Mice; D058977:Molecular Sequence Annotation; D020411:Oligonucleotide Array Sequence Analysis; D011485:Protein Binding; D055550:Protein Stability; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism*; D012708:Sertoli Cells / Q000166:cytology / Q000378:metabolism*; D013091:Spermatogenesis / Q000235:genetics*; D013739:Testosterone / Q000378:metabolism*; D014158:Transcription, Genetic
D015415:Biomarkers; D008024:Ligands; D011944:Receptors, Androgen; D013739:Testosterone
Androgen receptor; ST38c cells; Sertoli cells; Testis