Enzymatic modification of corn starch with 4-α-glucanotransferase results in increasing slow digestible and resistant starch.
In this study, partial 4-α-glucanotransferase (4αGT) treatment was used to modulate the fine structure responsible for the slow digestion and resistant property of starch. Normal corn starch modified using 4αGT for 4h showed an increase of slowly digestible starch from 9.40% to 20.92%, and resistant starch from 10.52 to 17.63%, respectively. The 4αGT treatment decreased the content of amylose from 32.6% to 26.8%. The molecular weight distribution and chain length distribution of 4αGT-treated starch showed a reduction of molecular weight and a great number of short (DP<13) and long (DP>30) chains through cleaving and reorganization of starch molecules. Both the short and long chain fractions of modified amylopectin were attributed to the low in vitro digestibility. The viscosity was inversely related to the digestibility of the 4αGT-treated starch. These results suggested that the 4αGT modified starch synthesized the novel amylopectin clusters with slow digestible and resistant character.
State Key Laboratory of Food Science & Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, PR China.
Huan
H
Jiang
State Key Laboratory of Food Science & Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, PR China. Electronic address: miaoming@jiangnan.edu.cn.
Ming
M
Miao
State Key Laboratory of Food Science & Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, PR China.
Fan
F
Ye
State Key Laboratory of Food Science & Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, PR China.
Bo
B
Jiang
State Key Laboratory of Food Science & Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, PR China.
Tao
T
Zhang
D000688:Amylose / Q000032:analysis; D000818:Animals; D001234:Aspergillus niger / Q000201:enzymology; D004063:Digestion; D006004:Glycogen Debranching Enzyme System / Q000378:metabolism*; D007455:Iodine / Q000737:chemistry; D008970:Molecular Weight; D013213:Starch / Q000737:chemistry* / Q000378:metabolism*; D014783:Viscosity; D003313:Zea mays / Q000737:chemistry*
D006004:Glycogen Debranching Enzyme System; D013213:Starch; D000688:Amylose; D007455:Iodine; C020569:4 alpha-glucanotransferase
4-α-Glucanotransferase; Amylose; Chain length; Molecular weight; Resistant starch; Slowly digestible starch