Expression of ZNF396 in basal cell carcinoma.
Zfp191 represses differentiation and keeps various cells in the stem/progenitor stage. Here, we report that a Zfp191 homolog protein, ZNF396, is expressed in basal cell carcinoma (BCC) and possibly represses the expression of a Notch system effector molecule, Hes1 (hairy and enhancer of split-1), and prevents BCC cells from undergoing Notch-mediated squamous cell differentiation. ZNF396 immunoreactivity was found in the nucleus of 35 of 38 cutaneous BCC and 4 of 74 squamous cell carcinoma tissue specimens. In non-tumorous epidermal tissues, ZNF396 immunoreactivity was restricted in basal cells. siRNA-mediated silencing of ZNF396 induced the expression of Notch2, Hes1, and involucrin in cultured BCC cells. Finally, we found that siRNA-mediated silencing of ZNF396 gene inhibited the proliferation of TE354.T basal cell carcinoma cells. ZNF396 might repress Notch-Hes1 signaling axis and prevent tumor cells from undergoing squamous differentiation in BCC.
Department of Immunopathology, Gifu University Graduate School of Medicine, Yanagido, Gifu, 501-1193, Japan.
Juncheng
J
Bai
Yusuke
Y
Kito
Hiroshi
H
Okubo
Tomoko
T
Nagayama
Tamotsu
T
Takeuchi
D051792:Basic Helix-Loop-Helix Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D002280:Basal Cell Carcinoma / Q000473:pathology*; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation; D057809:HEK293 Cells; D018398:Homeodomain Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D006801:Humans; D011498:Protein Precursors / Q000096:biosynthesis; D034622:RNA Interference; D034741:RNA, Small Interfering; D051883:Receptor, Notch2 / Q000096:biosynthesis; D012878:Skin Neoplasms / Q000473:pathology*; D000072056:Transcription Factor HES-1; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*
D051792:Basic Helix-Loop-Helix Proteins; D018398:Homeodomain Proteins; D011498:Protein Precursors; D034741:RNA, Small Interfering; D051883:Receptor, Notch2; D000072056:Transcription Factor HES-1; D014157:Transcription Factors; C497764:NOTCH2 protein, human; C476060:ZNF396 protein, human; C078493:HES1 protein, human; C032253:involucrin