[Effect of the steroid molecule structure on the direction of its hydroxylation by the fungus Curvularia lunata].
The main and side products of hydroxylation by the C. lunata VKPM F-981 mycelium of fourteen delta(4)-3-ketosteroids of the estrane, androstane, and pregnane series and six of their delta(5)-3beta-hydroxy analogues were identified by H1 PMR spectroscopy and comparison with standard samples. The obtained experimental data are considered in terms of the triangular model of the enzyme-substrate interaction. The dependence of the direction of hydroxylation of steroid molecules and the orientation of hydroxy groups on the structure of the initial substrate was revealed.
V A
VA
Andriushina
V V
VV
Iaderets
T S
TS
Stytsenko
A V
AV
Druzhinina
N E
NE
Voĭshvillo
D000731:Androstanes / Q000378:metabolism*; D002853:Chromatography, Liquid; D004578:Electron Spin Resonance Spectroscopy; D004962:Estranes / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D006900:Hydroxylation; D007664:Ketosteroids / Q000378:metabolism*; D015394:Molecular Structure; D025282:Mycelium / Q000378:metabolism*; D011278:Pregnanes / Q000378:metabolism*; D012015:Reference Standards; D004718:Saccharomycetales / Q000378:metabolism*; D013379:Substrate Specificity
D000731:Androstanes; D004962:Estranes; D005656:Fungal Proteins; D007664:Ketosteroids; D011278:Pregnanes