Fine structure of isolated mesophyll protoplasts of tobacco.
Protoplasts of palisade cells isolated enzymatically from mature leaves of tobacco were studied with the electron microscope. A cell wall was completely absent, and the chloroplasts contained large inclusion bodies which were believed to be a crystalline form of fraction I protein. The fine structure of the protoplasts was otherwise that of healthy mesophyll cells, indicating that they are in a good physiological state. Some protoplasts were multinucleate as a result of fusion during the isolation process.
Institute for Plant Virus Research, 959 Aobacho, 280, Chiba, Japan.
I
I
Takebe
Y
Y
Otsuki
Y
Y
Honda
T
T
Nishio
C
C
Matsui