Attenuation of hydrogen peroxide-mediated oxidative stress by Brassica juncea annexin-3 counteracts thiol-specific antioxidant (TSA1) deficiency in Saccharomyces cerevisiae.
Brassica juncea annexin-3 (BjAnn3) was functionally characterized for its ability to modulate H2O2-mediated oxidative stress in Saccharomyces cerevisiae. BjAnn3 showed a significant protective role in cellular-defense against oxidative stress and partially alleviated inhibition of mitochondrial respiration in presence of exogenously applied H2O2. Heterologous expression of BjAnn3 protected membranes from oxidative stress-mediated damage and positively regulated antioxidant gene expression for ROS detoxification. We conclude that, BjAnn3 partially counteracts the effects of thioredoxin peroxidase 1 (TSA1) deficiency and aids in cellular-protection across kingdoms. Despite partial compensation of TSA1 by BjAnn3 in cell-viability tests, the over-complementation in ROS-related features suggests the existence of both redundant (e.g. ROS detoxification) and distinct features (e.g. membrane protection versus proximity-based redox regulator) of both proteins.
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India. Electronic address: ahan.dalal@daad-alumni.de.
Ahan
A
Dalal
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India.
Abhaypratap
A
Vishwakarma
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India.
Naveen Kumar
NK
Singh
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India.
Triveni
T
Gudla
Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, India.
Mrinal Kanti
MK
Bhattacharyya
Department of Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad, Hyderabad, India.
Kollipara
K
Padmasree
Department of Plant Biochemistry and Physiology, Faculty of Biology, University of Bielefeld, Bielefeld, Germany.
Andrea
A
Viehhauser
Department of Plant Biochemistry and Physiology, Faculty of Biology, University of Bielefeld, Bielefeld, Germany.
Karl-Josef
KJ
Dietz
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India.
Pulugurtha Bharadwaja
PB
Kirti
D017318:Annexin A3 / Q000235:genetics / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D055786:Gene Knockout Techniques; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D009149:Mustard Plant / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D054464:Peroxiredoxins / Q000172:deficiency* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013438:Sulfhydryl Compounds / Q000378:metabolism*
D013438:Sulfhydryl Compounds; D006861:Hydrogen Peroxide; D054464:Peroxiredoxins; D017318:Annexin A3
Brassica juncea annexin-3; Complementation; Cross talk; Oxidative stress; Saccharomyces cerevisiae; Thioredoxin peroxidase 1