Fabrication and evaluation of porous beta-tricalcium phosphate/hydroxyapatite (60/40) composite as a bone graft extender using rat calvarial bone defect model.
Beta-tricalcium phosphate ( β -TCP) and hydroxyapatite (HA) are widely used as bone graft extenders due to their osteoconductivity and high bioactivity. This study aims to evaluate the possibility of using porous substrate with composite ceramics ( β -TCP: HA = 60% : 40%, 60TCP40HA) as a bone graft extender and comparing it with Bio-Oss. Interconnectivity and macroporosity of β -TCP porous substrate were 99.9% and 83%, respectively, and the macro-porosity of packed granule after crushing was 69%. Calvarial defect model with 8 mm diameter was generated with male Sprague-Dawley rats and 60TCP40HA was implanted. Bio-Oss was implanted for a control group and micro-CT and histology were performed at 4 and 8 weeks after implantation. The 60TCP40HA group showed better new bone formation than the Bio-Oss group and the bone formation at central area of bone defect was increased at 8 weeks in micro-CT and histology. The percent bone volume and trabecular number of the 60TCP40HA group were significantly higher than those of Bio-Oss group. This study confirms the usefulness of the porous 60TCP40HA composite as a bone graft extender by showing increased new bone formation in the calvarial defect model and improved bone formation both quantitatively and qualitatively when compared to Bio-Oss.
Department of Orthopedic Surgery, College of Medicine, SMG-SNU Boramae Medical Center, Seoul National University, Seoul 156-707, Republic of Korea ; Institute of Medical and Biological Engineering, Seoul National University Medical Research Center, Seoul 110-799, Republic of Korea.
Jae Hyup
JH
Lee
Research Center, Bioalpha, Sung-Nam 462-120, Republic of Korea.
Mi Young
MY
Ryu
Department of Orthopedic Surgery, College of Medicine, SMG-SNU Boramae Medical Center, Seoul National University, Seoul 156-707, Republic of Korea ; Institute of Medical and Biological Engineering, Seoul National University Medical Research Center, Seoul 110-799, Republic of Korea.
Hae-Ri
HR
Baek
Department of Orthopedic Surgery, College of Medicine, SMG-SNU Boramae Medical Center, Seoul National University, Seoul 156-707, Republic of Korea ; Research Center, Bioalpha, Sung-Nam 462-120, Republic of Korea.
Kyung Mee
KM
Lee
Research Center, Bioalpha, Sung-Nam 462-120, Republic of Korea.
Jun-Hyuk
JH
Seo
Department of Orthopedic Surgery, College of Medicine, SMG-SNU Boramae Medical Center, Seoul National University, Seoul 156-707, Republic of Korea ; Institute of Medical and Biological Engineering, Seoul National University Medical Research Center, Seoul 110-799, Republic of Korea.
Hyun-Kyung
HK
Lee
D000818:Animals; D018786:Bone Substitutes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D002130:Calcium Phosphates / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D004195:Disease Models, Animal; D017886:Durapatite / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D010012:Osteogenesis / Q000187:drug effects*; D016062:Porosity; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000293:injuries*
D018786:Bone Substitutes; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D017886:Durapatite