Protein and miRNA profiling of radiation-induced skin injury in rats: the protective role of peroxiredoxin-6 against ionizing radiation.
Radiation-induced skin injury is a serious concern during radiotherapy. However, the molecular mechanism underlying the pathogenesis of radiation-induced skin injury has not been extensively reported. Most biological functions are performed and regulated by proteins and noncoding RNAs, including microRNAs (miRNAs). The interplay between mRNA and miRNA has been implicated in disease initiation and progression. Technical advances in genomics and proteomics have enabled the exploration of the etiology of diseases and have the potential to broaden our understanding of the molecular pathogenesis of radiation-induced skin injury. In this study, we compared the protein and miRNA expression in rat skin irradiated with a 45-Gy electron beam with expression from adjacent normal tissues. We found 24 preferentially expressed proteins and 12 dysregulated miRNAs in irradiated skin. By analyzing the protein and miRNA profiles using bioinformatics tools, we identified a possible interaction between miR-214 and peroxiredoxin-6 (PRDX-6). Next, we investigated the expression of PRDX-6 and the consequences of its dysregulation. PRDX-6 is suppressed by radiation-inducible miR-214 and is involved in the pathogenesis of radiation-induced skin injury. Overexpression of PRDX-6 conferred radioresistance on cells, decreased cell apoptosis, and preserved mitochondrial integrity after radiation exposure. In addition, in vivo transfection with PRDX-6 reduced radiation-induced reactive oxygen species and the malondialdehyde concentration and ameliorated radiation-induced skin damage in rats. Our present findings illustrate the molecular changes during radiation-induced skin injury and the important role of PRDX-6 in ameliorating this damage in rats.
School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou 215123, China. Electronic address: zhang.shuyu@hotmail.com.
Shuyu
S
Zhang
Cyrus Tang Hematology Center, Soochow University, Suzhou 215123, China.
Wenjie
W
Wang
School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou 215123, China.
Qing
Q
Gu
School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou 215123, China.
Jiao
J
Xue
School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou 215123, China.
Han
H
Cao
School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou 215123, China.
Yiting
Y
Tang
Department of General Surgery, Second Affiliated Hospital, Soochow University, Suzhou 215004, China.
Xiaohui
X
Xu
School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou 215123, China. Electronic address: jpcao@suda.edu.cn.
Jianping
J
Cao
Department of Radio-oncology, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou 215001, China.
Jundong
J
Zhou
Department of Radio-oncology, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou 215001, China.
Jinchang
J
Wu
Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
Wei-Qun
WQ
Ding
D000818:Animals; D005786:Gene Expression Regulation / Q000528:radiation effects; D035683:MicroRNAs / Q000096:biosynthesis*; D054465:Peroxiredoxin VI / Q000096:biosynthesis* / Q000235:genetics; D014176:Protein Biosynthesis / Q000528:radiation effects*; D011836:Radiation Tolerance / Q000235:genetics; D011839:Radiation, Ionizing; D051381:Rats; D012867:Skin / Q000293:injuries / Q000378:metabolism* / Q000473:pathology / Q000528:radiation effects; D059467:Transcriptome
D035683:MicroRNAs; D054465:Peroxiredoxin VI; C515984:Prdx6 protein, rat
Free radicals; Peroxiredoxin-6; Protein and miRNA profiling; Radiation-induced skin injury