Bioactive nanocomposite PLDL/nano-hydroxyapatite electrospun membranes for bone tissue engineering.
New nanocomposite membranes with high bioactivity were fabricated using the electrospinning. These nanocomposites combine a degradable polymer poly(L/DL)-lactide and bone cell signaling carbonate nano-hydroxyapatite (n-HAp). Chemical and physical characterization of the membranes using scanning electron microscopy, Fourier transform infrared spectroscopy and the wide angle X-ray diffraction evidenced that nanoparticles were successfully incorporated into the fibers and membrane structure. The incorporation of the n-HAp into the structure increased significantly the mineralization of the membrane in vitro. It has been demonstrated that after a 3-day incubation of composite membrane in the Simulated Body Fluid a continuous compact apatite layer was formed. In vitro experiments demonstrated that the incorporation of n-HAp significantly improved cell attachment, upregulated cells proliferation and stimulated cell differentiation quantified using Alkaline Phosphatase and OsteoImage tests. In conclusion, the results demonstrated that the addition of n-HAp provided chemical cues that were a key factor that regulated osteoblastic differentiation.
Division of Materials Engineering, Department of Mechanical Engineering Fundamentals, ATH University of Bielsko-Biala, Willowa 2 Street, 43-309, Bielsko-Biała, Poland, irajzer@ath.bielsko.pl.
Izabella
I
Rajzer
Elżbieta
E
Menaszek
Ryszard
R
Kwiatkowski
Wojciech
W
Chrzanowski
D001672:Biocompatible Materials / Q000138:chemical synthesis; D001826:Body Fluids / Q000737:chemistry; D001846:Bone Development / Q000502:physiology*; D018786:Bone Substitutes / Q000138:chemical synthesis*; D002454:Cell Differentiation; D002460:Cell Line; D002470:Cell Survival; D002478:Cells, Cultured; D017886:Durapatite / Q000737:chemistry*; D004595:Electroplating / Q000379:methods; D004867:Equipment Design; D006801:Humans; D008422:Materials Testing; D008567:Membranes, Artificial; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology; D012399:Rotation; D013499:Surface Properties; D023822:Tissue Engineering / Q000295:instrumentation; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D018786:Bone Substitutes; D008567:Membranes, Artificial; D017886:Durapatite