MiR-874 promotes intestinal barrier dysfunction through targeting AQP3 following intestinal ischemic injury.
Intestinal ischemic injury is a significant clinical problem arising from diseases or as a complication of abdominal surgery. Our previous study showed aquaporin 3 is involved in intestinal barrier impairment. Here, we revealed that intestinal ischemia induced a time-dependent increase of miR-874 expression and a time-dependent decrease of AQP3 expression, and the level of miR-874 expression was inversely related to AQP3 protein expression. In addition, miR-874 promoted the paracellular permeability in vitro through targeting 3'UTR of AQP3. Two of the tight junction proteins, Occludin and Claudin-1, were found to be involved in miR-874-induced intestinal barrier dysfunction.
Division of Gastrointestinal Surgery, Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Xiaofei
X
Zhi
Division of Gastrointestinal Surgery, Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Jinqiu
J
Tao
Division of Gastrointestinal Surgery, Department of General Surgery, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu, China.
Zengliang
Z
Li
Division of Gastrointestinal Surgery, Department of General Surgery, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu, China.
Baofei
B
Jiang
Department of Surgery, The First People Hospital of Changzhou, Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China.
Jin
J
Feng
Division of Gastrointestinal Surgery, Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Li
L
Yang
Division of Gastrointestinal Surgery, Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China. Electronic address: brightmoon_1@sina.com.
Hao
H
Xu
Division of Gastrointestinal Surgery, Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China. Electronic address: xuzekuan@njmu.edu.cn.
Zekuan
Z
Xu
D020413:3' Untranslated Regions; D000818:Animals; D051400:Aquaporin 3 / Q000235:genetics* / Q000378:metabolism; D018988:Bacterial Translocation; D001483:Base Sequence; D018938:Caco-2 Cells; D015687:Cell Hypoxia; D062445:Claudin-1 / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000098:blood supply / Q000473:pathology; D007511:Ischemia / Q000378:metabolism*; D008638:Mesenteric Arteries / Q000473:pathology; D008641:Mesenteric Vascular Occlusion / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000502:physiology*; D062793:Occludin / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D019108:Tight Junctions / Q000378:metabolism*
D020413:3' Untranslated Regions; C496028:Aqp3 protein, mouse; D062445:Claudin-1; C568113:Cldn1 protein, mouse; C586538:MIRN874 microRNA, mouse; D035683:MicroRNAs; D062793:Occludin; C568986:Ocln protein, mouse; D051400:Aquaporin 3
Aquaporin; Intestinal barrier; Intestinal ischemia; MiR-874