[The identification of Saccharomyces cerevisiae genes leading to synthetic lethality of prion [PSI+] with Sup45 mutations].
Previously, we proposed a test system allowing to perform search for genes that influence the properties of the Sup35 and Sup45 protein. This test is based on the phenomenon of lethality of diploids that combine mutations in SUP45 gene with [PSI+] prion. Lethality of this combination depends on the type of sup45 mutation, and the properties of the prion. [PSI+] variant, which is a strong suppressor ([PSI+]s), showing synthetic lethality with all the nonsense mutations and some missense sup45 mutations in the heterozygote state. The presence of extra copies of a gene under test that affects the phenotypic manifestation of prion [PSI+] or translation termination factors properties, leads to the increase or decrease in diploid lethality. Gene library screening using this test system allowed us to establish the effect of ten fragments of genomic DNA of yeast on synthetic lethality. Deletion analysis of these regions has led to the identification of the HLJ1 and TEF2 genes, as affecting Sup35 protein prionization and/or the efficiency of translation termination.
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Matveenko
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Zemlianko
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Zhuravleva
D004171:Diploidy; D015966:Gene Expression Regulation, Fungal; D015723:Gene Library; D005804:Genes, Lethal; D050956:HSP40 Heat-Shock Proteins / Q000235:genetics; D026341:Minichromosome Maintenance 1 Protein / Q000235:genetics; D009154:Mutation; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000235:genetics; D010454:Peptide Termination Factors / Q000235:genetics*; D011328:Prions / Q000235:genetics; D014176:Protein Biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*
C586861:HLJ1 protein, S cerevisiae; D050956:HSP40 Heat-Shock Proteins; C498030:MCM1 protein, S cerevisiae; D026341:Minichromosome Maintenance 1 Protein; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D010454:Peptide Termination Factors; D011328:Prions; C080775:SUP45 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C057183:TEF2 protein, S cerevisiae