The effect of polyglycerol sulfate branching on inflammatory processes.
In this study, the extent to which the scaffold architecture of polyglycerol sulfates affects inflammatory processes and hemocompatibility is investigated. Competitive L-selectin binding assays, cellular uptake studies, and blood compatibility readouts are done to evaluate distinct biological properties. Fully glycerol based hyperbranched polyglycerol architectures are obtained by either homopolymerization of glycidol (60% branching) or a new copolymerization strategy of glycidol with ethoxyethyl glycidyl ether. Two polyglycerols with 24 and 42% degree of branching (DB) are synthesized by using different monomer feed ratios. A perfectly branched polyglycerol dendrimer is synthesized according to an iterative two-step protocol based on allylation of the alcohol and subsequent catalytic dihydroxylation. All the polyglycerol sulfates are synthesized with a comparable molecular weight and degree of sulfation. The DB make the different polymer conjugates perform different ways. The optimal DB is 60% in all biological assays.
Freie Universität Berlin, Institut für Chemie und Biochemie, Takustraße 3, 14195, Berlin, Germany.
Florian
F
Paulus
Ronny
R
Schulze
Dirk
D
Steinhilber
Maximilian
M
Zieringer
Ingo
I
Steinke
Pia
P
Welker
Kai
K
Licha
Stefanie
S
Wedepohl
Jens
J
Dernedde
Rainer
R
Haag
D050091:Dendrimers / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D005990:Glycerol / Q000737:chemistry* / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism*; D019041:L-Selectin / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D013194:Staining and Labeling / Q000379:methods; D013431:Sulfates / Q000737:chemistry* / Q000378:metabolism
D050091:Dendrimers; D011108:Polymers; D013431:Sulfates; D019041:L-Selectin; C043941:polyglycerol; D005990:Glycerol
anti-inflammatory drugs; biological properties; blood compatibility; copolymerization; glycidol