Evaluation of in vitro antioxidant and apoptotic activities of Cyperus rotundus.
OBJECTIVE
To evaluate in vitro antioxidant and apoptotic activities of Cyperus rotundus (C. rotundus).


METHODS
The phytochemical study and the antioxidant activities of both methanol and aqueous extracts from C. rotundus aerial part were determined. In addition, these extracts were also investigated for their cytotoxic and apoptotic activities. The major compound of the methanol extract was isolated. Both methanol and aqueous extracts (300, 150, and 50 μg/mL) were evaluated for their antioxidant activity by the xanthine/xanthine oxidase assay system. However, 16, 8, and 4 mg/mL of each extract were tested to investigate their OH. formation scavenging potential. Aqueous extract (800, 400, and 200 μg/mL) and methanol extract (350, 175, and 88 μg/mL) were tested against lipid peroxidation, induced by 75 μM H2O2. The cytotoxicity (by MTT assay) and cell DNA fragmentation of both extracts were evaluated towards K562 and L1210 cell lines. The major compound was obtained from the butanol fraction of methanol extract and its structure was determined by RMN spectroscopic analysis.


RESULTS
The methanol and aqueous extracts showed respectively, 88% and 19% inhibition of xanthine oxidase activity. Yet, the same extracts inhibited lipid peroxidation by 61.5% and 42.0%, respectively. Both extracts inhibited OH. formation by 27.1% and 25.3%, respectively. Only methanol extract induced DNA degradation. Orientin was determined as the major compound isolated from the butanol fraction of methanol extract.


CONCLUSIONS
It appears that C. rotundus extracts exhibit a potential use as a natural antioxidant and an apoptosis inducer.
Unité de Substances Naturelles Bioactives et Biotechnologies UR12ES12, Université de Monastir, Faculté de Pharmacie de Monastir, Rue Avicenne, 5019 Monastir, Tunisie; Laboratoire de Biologie Moléculaire et Cellulaire, Faculté de Médecine Dentaire de Monastir, Universitéde Monastir, Rue Avicenne, 5019 Monastir, Tunisie.
Kilani-Jaziri
KJ
Soumaya
Unité de Substances Naturelles Bioactives et Biotechnologies UR12ES12, Université de Monastir, Faculté de Pharmacie de Monastir, Rue Avicenne, 5019 Monastir, Tunisie; Laboratoire de Biologie Moléculaire et Cellulaire, Faculté de Médecine Dentaire de Monastir, Universitéde Monastir, Rue Avicenne, 5019 Monastir, Tunisie.
Ghedira
G
Zied
Unité de Substances Naturelles Bioactives et Biotechnologies UR12ES12, Université de Monastir, Faculté de Pharmacie de Monastir, Rue Avicenne, 5019 Monastir, Tunisie; Laboratoire de Biologie Moléculaire et Cellulaire, Faculté de Médecine Dentaire de Monastir, Universitéde Monastir, Rue Avicenne, 5019 Monastir, Tunisie.
Nasr
N
Nouha
Unité de Substances Naturelles Bioactives et Biotechnologies UR12ES12, Université de Monastir, Faculté de Pharmacie de Monastir, Rue Avicenne, 5019 Monastir, Tunisie; Laboratoire de Biologie Moléculaire et Cellulaire, Faculté de Médecine Dentaire de Monastir, Universitéde Monastir, Rue Avicenne, 5019 Monastir, Tunisie.
Krifa
K
Mounira
Unité de Substances Naturelles Bioactives et Biotechnologies UR12ES12, Université de Monastir, Faculté de Pharmacie de Monastir, Rue Avicenne, 5019 Monastir, Tunisie.
Ghedira
G
Kamel
Laboratoire de Botanique, Pharmacognosie et Phytothérapie, UMR CNRS 5557 Ecologie Microbienne; Institut des Sciences Pharmaceutiques et Biologiques-Faculté de Pharmacie Université Claude Bernard, Bâtiment Nétien-8 Avenue Rockefeller-69373 Lyon cedex 08, France.
Franca Dijoux Marie
FD
Genviève
Laboratoire de Biologie Moléculaire et Cellulaire, Faculté de Médecine Dentaire de Monastir, Universitéde Monastir, Rue Avicenne, 5019 Monastir, Tunisie. Electronic address: leila.chekir@laposte.net.
Ghedira Chekir
GC
Leila
D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D029786:Cyperus / Q000737:chemistry*; D005419:Flavonoids; D005960:Glucosides; D006801:Humans; D020014:K562 Cells; D015227:Lipid Peroxidation / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D059808:Polyphenols / Q000737:chemistry / Q000494:pharmacology; D014969:Xanthine Oxidase / Q000378:metabolism
D000975:Antioxidants; D005419:Flavonoids; D005960:Glucosides; D010936:Plant Extracts; D059808:Polyphenols; D014969:Xanthine Oxidase; C065886:orientin
DNA fragmentation; Deoxyribose assay; Lipid peroxidation assay; Orientin; Polyphenols; Xanthine/Xanthine oxidase assay