Recycling endosome tubule morphogenesis from sorting endosomes requires the kinesin motor KIF13A.
Early endosomes consist of vacuolar sorting and tubular recycling domains that segregate components fated for degradation in lysosomes or reuse by recycling to the plasma membrane or Golgi. The tubular transport intermediates that constitute recycling endosomes function in cell polarity, migration, and cytokinesis. Endosomal tubulation and fission require both actin and intact microtubules, but although factors that stabilize recycling endosomal tubules have been identified, those required for tubule generation from vacuolar sorting endosomes (SEs) remain unknown. We show that the microtubule motor KIF13A associates with recycling endosome tubules and controls their morphogenesis. Interfering with KIF13A function impairs the formation of endosomal tubules from SEs with consequent defects in endosome homeostasis and cargo recycling. Moreover, KIF13A interacts and cooperates with RAB11 to generate endosomal tubules. Our data illustrate how a microtubule motor couples early endosome morphogenesis to its motility and function.
Institut Curie, Centre de Recherche, Paris 75248, France; Structure and Membrane Compartments, CNRS UMR144, Paris 75248, France. Electronic address: cedric.delevoye@curie.fr.
Cédric
C
Delevoye
Institut Curie, Centre de Recherche, Paris 75248, France; Molecular Mechanisms of Intracellular Transport, CNRS UMR144, Paris 75248, France.
Stéphanie
S
Miserey-Lenkei
Institut Curie, Centre de Recherche, Paris 75248, France; Membrane and Cytoskeleton Dynamics, CNRS UMR144, Paris 75248, France.
Guillaume
G
Montagnac
Institut Curie, Centre de Recherche, Paris 75248, France; Structure and Membrane Compartments, CNRS UMR144, Paris 75248, France.
Floriane
F
Gilles-Marsens
Institut Curie, Centre de Recherche, Paris 75248, France; Cell and Tissue Imaging Facility (PICT-IBiSA), CNRS UMR144, Paris 75248, France.
Perrine
P
Paul-Gilloteaux
Institut Curie, Centre de Recherche, Paris 75248, France; Structure and Membrane Compartments, CNRS UMR144, Paris 75248, France.
Francesca
F
Giordano
Institut Curie, Centre de Recherche, Paris 75248, France; Cell and Tissue Imaging Facility (PICT-IBiSA), CNRS UMR144, Paris 75248, France.
François
F
Waharte
Department of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia and University of Pennsylvania, Philadelphia, PA 19104, USA.
Michael S
MS
Marks
Institut Curie, Centre de Recherche, Paris 75248, France; Molecular Mechanisms of Intracellular Transport, CNRS UMR144, Paris 75248, France.
Bruno
B
Goud
Institut Curie, Centre de Recherche, Paris 75248, France; Structure and Membrane Compartments, CNRS UMR144, Paris 75248, France; Cell and Tissue Imaging Facility (PICT-IBiSA), CNRS UMR144, Paris 75248, France.
Graça
G
Raposo
D004705:Endocytosis; D011992:Endosomes / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D016547:Kinesins / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism / Q000648:ultrastructure; D009024:Morphogenesis; D011485:Protein Binding; D021381:Protein Transport; D020691:rab GTP-Binding Proteins / Q000378:metabolism; D000099193:rab11 GTP-Binding Proteins
D016547:Kinesins; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins; C417670:KIF13A protein, human; C583168:KIF13A protein, mouse