A new colorimetric strategy for monitoring caspase 3 activity by HRP-mimicking DNAzyme-peptide conjugates.
A new method for caspase 3 activity assay has been developed based on HRP-mimicking DNAzyme-peptide conjugates. The mechanism of detection was based on the specific cleavage of DEVD-peptides by active caspase 3 for recognition and the catalytic properties of HRP-mimicking DNAzymes for signal amplification. Under optimal conditions, the detection limit of caspase 3 was 0.89 nM. The proposed method was also successfully applied for the detection of caspase 3 in apoptosis cell lysates.
Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing 100084, China. tongaj@mail.tsinghua.edu.cn.
Zhaojuan
Z
Zhou
Lu
L
Peng
Xiaoyan
X
Wang
Yu
Y
Xiang
Aijun
A
Tong
D015374:Biosensing Techniques / Q000379:methods*; D053148:Caspase 3 / Q000032:analysis / Q000378:metabolism*; D003124:Colorimetry / Q000379:methods; D021881:DNA, Catalytic / Q000032:analysis / Q000378:metabolism*; D004789:Enzyme Activation / Q000502:physiology; D006367:HeLa Cells; D006735:Horseradish Peroxidase / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D010446:Peptide Fragments / Q000032:analysis / Q000378:metabolism*
D021881:DNA, Catalytic; D010446:Peptide Fragments; D006735:Horseradish Peroxidase; D053148:Caspase 3