Different approaches for obtaining antibodies from human B cells.
Antibodies have emerged as powerful therapeutics effective for treating a number of human conditions and diseases. While early successes utilized small animals to generate therapeutic antibodies, human antibodies are now preferred in order to limit anti-antibody immune responses. Antibodies with human amino acid sequences can be generated in a number of ways, such as humanizing antibodies from other species or expressing human antibodies in transgenic animals. This review focuses on methods for obtaining antibodies directly from human B cells. These methods use both antigen exposed and non-exposed ("naïve") humans as B cell sources, and apply various technologies to isolate desired antibodies; including cell line generation, single cell isolation, display technologies, and B cell library generation.
Marcus R
MR
Duvall
13 Lowndes Pointe Drive, Charleston, 29403, USA. ryan_fiorini@hotmail.com.
Ryan N
RN
Fiorini
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D061505:Cell Surface Display Techniques; D006801:Humans
D000906:Antibodies