Expression and characterization of Coprothermobacter proteolyticus alkaline serine protease.
A putative protease gene (aprE) from the thermophilic bacterium Coprothermobacter proteolyticus was cloned and expressed in Bacillus subtilis. The enzyme was determined to be a serine protease based on inhibition by PMSF. Biochemical characterization demonstrated that the enzyme had optimal activity under alkaline conditions (pH 8-10). In addition, the enzyme had an elevated optimum temperature (60°C). The protease was also stable in the presence of many surfactants and oxidant. Thus, the C. proteolyticus protease has potential applications in industries such as the detergent market.
National Institute for Biotechnology and Genetic Engineering (NIBGE), P.O. Box 577, Jhang Road, Faisalabad 38000, Pakistan ; Pakistan Institute of Engineering and Applied Science (PIEAS), Islamabad 44000, Pakistan.
Tanveer
T
Majeed
National Institute for Biotechnology and Genetic Engineering (NIBGE), P.O. Box 577, Jhang Road, Faisalabad 38000, Pakistan ; Pakistan Institute of Engineering and Applied Science (PIEAS), Islamabad 44000, Pakistan.
Romana
R
Tabassum
USDA-ARS, Bioproduct Chemistry and Engineering Research Unit, 800 Buchanan Street, Albany, CA 94710, USA.
William J
WJ
Orts
USDA-ARS, Bioproduct Chemistry and Engineering Research Unit, 800 Buchanan Street, Albany, CA 94710, USA.
Charles C
CC
Lee
D001412:Bacillus subtilis; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D019295:Computational Biology; D004926:Escherichia coli; D005822:Genetic Vectors / Q000235:genetics; D006089:Gram-Negative Anaerobic Bacteria / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D012697:Serine Endopeptidases / Q000235:genetics* / Q000378:metabolism*; D013696:Temperature
D001426:Bacterial Proteins; D012697:Serine Endopeptidases