A particular set of small non-coding RNAs is bound to the distinctive Argonaute protein of Trypanosoma cruzi: insights from RNA-interference deficient organisms.
The study of small RNAs and Argonaute proteins in eukaryotes that are deficient in functional RNA interference could provide insights into novel functions of small RNAs. In this study we describe small non-coding RNAs bound to a distinctive Argonaute protein of Trypanosoma cruzi, TcPIWI-tryp. Co-immunoprecipitation of TcPIWI-tryp followed by deep sequencing of isolated RNA identified abundant small RNAs derived from rRNAs and tRNAs. The small RNA repertoire differed from that of the canonical Argonaute in organisms with functional RNA interference, which could indicate novel biological functions for TcPIWI-tryp in T. cruzi and other members of the trypanosomatid clade.
Laboratory of Functional Genomics, Institut Pasteur of Montevideo, Montevideo, Uruguay. Electronic address: rgarcia@pasteur.edu.uy.
Maria Rosa
MR
Garcia-Silva
Laboratory of Functional Genomics, Institut Pasteur of Montevideo, Montevideo, Uruguay.
Julia
J
Sanguinetti
Laboratory of Functional Genomics, Institut Pasteur of Montevideo, Montevideo, Uruguay.
Florencia
F
Cabrera-Cabrera
Icahn School of Medicine at Mount Sinai, Department of Genetics and Genomic Sciences, New York, NY, USA.
Oscar
O
Franzén
Laboratory of Functional Genomics, Institut Pasteur of Montevideo, Montevideo, Uruguay; Department of Medicine, Hospital de Clínicas, Montevideo, Uruguay. Electronic address: cayota@pasteur.edu.uy.
Alfonso
A
Cayota
D000818:Animals; D060565:Argonaute Proteins / Q000378:metabolism*; D001483:Base Sequence; D008969:Molecular Sequence Data; D011485:Protein Binding; D015800:Protozoan Proteins / Q000378:metabolism*; D034622:RNA Interference; D012323:RNA Processing, Post-Transcriptional; D016053:RNA, Protozoan / Q000235:genetics* / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000235:genetics / Q000378:metabolism; D058727:RNA, Small Untranslated / Q000235:genetics* / Q000378:metabolism*; D012343:RNA, Transfer / Q000235:genetics / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000235:genetics* / Q000378:metabolism*
D060565:Argonaute Proteins; D015800:Protozoan Proteins; D016053:RNA, Protozoan; D012335:RNA, Ribosomal; D058727:RNA, Small Untranslated; D012343:RNA, Transfer
Argonaute proteins; Small non-coding RNAs; Trypanosoma cruzi; rRNAs; tRNA-derived fragments