Laser-induced tissue fluorescence in radiofrequency tissue-fusion characterization.
Heat-induced tissue fusion is an important procedure in modern surgery and can greatly reduce trauma, complications, and mortality during minimally invasive surgical blood vessel anastomosis, but it may also have further benefits if applied to other tissue types such as small and large intestine anastomoses. We present a tissue-fusion characterization technology using laser-induced fluorescence spectroscopy, which provides further insight into tissue constituent variations at the molecular level. In particular, an increase of fluorescence intensity in 450- to 550-nm range for 375- and 405-nm excitation suggests that the collagen cross-linking in fused tissues increased. Our experimental and statistical analyses showed that, by using fluorescence spectral data, good fusion could be differentiated from other cases with an accuracy of more than 95%. This suggests that the fluorescence spectroscopy could be potentially used as a feedback control method in online tissue-fusion monitoring.
Imperial College London, St. Mary's Hospital, Department of Surgery and Cancer, London W2 1NY, United KingdombUniversity of Liverpool, Department of Electrical Engineering and Electronics, Liverpool L69 3GJ, United KingdomcUniversity of Liverpool, Departm.
Lei
L
Su
Imperial College London, St. Mary's Hospital, Department of Surgery and Cancer, London W2 1NY, United KingdombUniversity of Liverpool, Department of Electrical Engineering and Electronics, Liverpool L69 3GJ, United Kingdom.
Martina B
MB
Fonseca
Imperial College London, St. Mary's Hospital, Department of Surgery and Cancer, London W2 1NY, United Kingdom.
Shobhit
S
Arya
Imperial College London, Department of Medicine, London W2 1NY, United Kingdom.
Hiromi
H
Kudo
Imperial College London, Department of Medicine, London W2 1NY, United Kingdom.
Robert
R
Goldin
Imperial College London, St. Mary's Hospital, Department of Surgery and Cancer, London W2 1NY, United Kingdom.
George B
GB
Hanna
Imperial College London, St. Mary's Hospital, Department of Surgery and Cancer, London W2 1NY, United KingdombUniversity of Liverpool, Department of Electrical Engineering and Electronics, Liverpool L69 3GJ, United Kingdom.
Daniel S
DS
Elson
D000714:Anastomosis, Surgical / Q000379:methods*; D000818:Animals; D001794:Blood Pressure; D001808:Blood Vessels / Q000473:pathology; D003094:Collagen / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D004867:Equipment Design; D006358:Hot Temperature; D007420:Intestine, Large / Q000473:pathology* / Q000601:surgery; D007421:Intestine, Small / Q000473:pathology* / Q000601:surgery; D007834:Lasers; D008856:Microscopy, Fluorescence; D011846:Radio Waves; D012044:Regression Analysis; D013050:Spectrometry, Fluorescence; D013552:Swine
D003432:Cross-Linking Reagents; D003094:Collagen