Different effects of a novel CaO-MgO-SiO₂-based multiphase glass-ceramic on cell behaviors of normal and cancer cells in vitro.
The effects in vitro of a novel multiphase glass-ceramic (with nominal composition of 43.19% CaO, 7.68% MgO, and 49.13% SiO2 in weight percent) on cell adhesion, proliferation, differentiation and ultrastructure of human osteosarcoma cell line MG63, mouse fibroblasts L929, and human lung adenocarcinoma epithelial cell line A549 were investigated in this research. Scanning electron microscopy (SEM) micrographs revealed that the surface morphology of this glass-ceramic was beneficial to cell adhesion. The glass-ceramic extracts at certain concentrations could stimulate the proliferation and differentiation of MG63 and L929 cells, whereas inhibit A549 proliferation, which might be resulted from the released Si ions. In addition, when cultured with 0.1mg/mL glass-ceramic powder suspension, the cell ultrastructure of MG63 showed abundant organelles and L929 displayed the phenomena of cellular stress response. While more interestingly, A549 exhibited chromatin condensation, mitochondria swell and RER expansion, which was presumed to be early signs of apoptosis. These results suggest that this novel CaO-MgO-SiO2-based multiphase glass-ceramic has potential for bone regeneration and tissue engineering applications.
College of Materials Science and Engineering, Sichuan University, Chengdu 610064, PR China.
Mengjiao
M
Zhang
College of Materials Science and Engineering, Sichuan University, Chengdu 610064, PR China.
Xianchun
X
Chen
College of Materials Science and Engineering, Sichuan University, Chengdu 610064, PR China.
Ximing
X
Pu
College of Materials Science and Engineering, Sichuan University, Chengdu 610064, PR China.
Xiaoming
X
Liao
College of Materials Science and Engineering, Sichuan University, Chengdu 610064, PR China.
Zhongbing
Z
Huang
College of Materials Science and Engineering, Sichuan University, Chengdu 610064, PR China. Electronic address: nic0700@scu.edu.cn.
Guangfu
G
Yin
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002516:Ceramics / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005898:Glass / Q000737:chemistry*; D006801:Humans; D051379:Mice; D009369:Neoplasms / Q000473:pathology*; D010316:Particle Size; D013329:Structure-Activity Relationship; D013499:Surface Properties
C509301:CaO-MgO-SiO2 glass
Adhesion; CaO–MgO–SiO(2) glass–ceramic; Differentiation; Proliferation; Ultrastructure