Antioxidant and orofacial anti-nociceptive activities of the stem bark aqueous extract of Anadenanthera colubrina (Velloso) Brenan (Fabaceae).
The anti-nociceptive and antioxidant activities of the Anadenantheracolubrina stem bark aqueous extract (AEAC) were investigated. AEAC (30 μg/mL) reduced 94.8% of 2,2-diphenyl-1-picrylhydrazyl radical and prevented 64% (200 μg/mL) of lipid peroxidation caused by 2,2'-azobis(2-methylpropionamidine) dihydrochloride-induced peroxyl radicals. AEAC treatment (200 and 400 mg/kg) significantly (p < 0.001) reduced mice orofacial nociception in the first (61.4% and 62.6%, respectively) and second (48.9% and 61.9%, respectively) phases of the formalin test. Nociception caused by glutamate was significantly (p < 0.001) reduced by up to 79% at 400 mg/kg, while 56-60% of the nociceptive behaviour induced by capsaicin was significantly inhibited by AEAC (100-400 mg/kg). Mice treated with AEAC did not show changes in motor performance in the Rota-rod apparatus. It appears that AEAC is of pharmacological importance in treating pain due to its anti-nociceptive effects, which were shown to be mediated by central and peripheral mechanisms.
a Department of Physiology , Federal University of Sergipe , São Cristovão , SE , Brazil.
N P
NP
Damascena
M T S
MT
Souza
A F
AF
Almeida
R S
RS
Cunha
N P
NP
Damascena
R L
RL
Curvello
A C B
AC
Lima
E C V
EC
Almeida
C C S
CC
Santos
A S
AS
Dias
M S
MS
Paixão
L M A
LM
Souza
L J
LJ
Quintans Júnior
C S
CS
Estevam
B S
BS
Araujo
D000578:Amidines / Q000494:pharmacology; D000700:Analgesics / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D001713:Biphenyl Compounds / Q000494:pharmacology; D002211:Capsaicin / Q000494:pharmacology; D031953:Colubrina / Q000737:chemistry*; D007887:Fabaceae / Q000737:chemistry*; D018698:Glutamic Acid / Q000494:pharmacology; D015227:Lipid Peroxidation; D008297:Male; D051379:Mice; D010146:Pain / Q000188:drug therapy; D010147:Pain Measurement; D008517:Phytotherapy; D010851:Picrates / Q000494:pharmacology; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology; D018547:Plant Stems / Q000737:chemistry
D000578:Amidines; D000700:Analgesics; D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; D018698:Glutamic Acid; C046728:2,2'-azobis(2-amidinopropane); C004931:1,1-diphenyl-2-picrylhydrazyl; D002211:Capsaicin
Anadenanthera colubrina; Fabaceae; capsaicin; formalin; glutamate; orofacial pain