Chemopreventive effect of Mentha piperita on dimethylbenz[a]anthracene and formaldehyde-induced tongue carcinogenesis in mice (histological and immunohistochemical study).
OBJECTIVE
Cancer chemoprevention is defined as the use of chemicals or dietary components to block, inhibit, or reverse the development of cancer in normal or pre-neoplastic tissue. Mentha extract (ME) has antioxidant and antiperoxidant properties. This study was held to investigate the protective and anticancer effect of Mentha leaves aqueous extract on oral epithelium of mice tongues.


METHODS
A total of 80 Egyptian albino mice were divided into three groups. Group I served as control (not subjected to any kind of treatment), and groups II and III were subjected to two-stage chemical carcinogenesis through topical application of dimethylbenz[a]anthracene (DMBA) followed by formaldehyde on dorsal and ventral surfaces of tongues for 9 weeks. Mentha leaves extract was administrated to group III at the same time of cancer induction. Histological changes were assessed in H&E sections at 3-week intervals. The anticarcinogenic effect of Mentha piperita was tested using immunostain with anticaspase antibody.


RESULTS
The oral administration of ME reduced the appearance of dysplastic cellular changes with 61% and inhibited tumor incidence with 100%. Group I showed moderate-to-strong cytoplasmic caspase expression. At 6-week interval, group II showed weak-to-moderate caspase expression, while sections from group III showed moderate-to-strong caspase expression. High significant statistical difference in the total score of caspase 3 expression was found between specimens obtained from animals sacrificed at 6 weeks in groups I, II, and III (P = 0.001**).


CONCLUSIONS
Our study demonstrated that Mentha piperita has inhibited the initiation and promotion of oral dysplastic lesions.
Faculty of Oral & Dental Medicine, Department of Oral Pathology, Cairo University, Cairo, Egypt.
Rehab F
RF
Kasem
Radwa H
RH
Hegazy
Mona A A
MA
Arafa
Mona M
MM
AbdelMohsen
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000009:adverse effects*; D000818:Animals; D016588:Anticarcinogenic Agents / Q000627:therapeutic use*; D000975:Antioxidants / Q000627:therapeutic use; D001485:Basement Membrane / Q000187:drug effects / Q000473:pathology; D063646:Carcinogenesis / Q000187:drug effects*; D002273:Carcinogens / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D053148:Caspase 3 / Q000032:analysis; D018890:Chemoprevention; D003238:Connective Tissue / Q000187:drug effects / Q000473:pathology; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D005557:Formaldehyde / Q000009:adverse effects*; D006965:Hyperplasia; D007633:Keratins; D008297:Male; D036142:Mentha piperita; D051379:Mice; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000627:therapeutic use*; D020011:Protective Agents / Q000627:therapeutic use; D014059:Tongue / Q000187:drug effects / Q000473:pathology; D014062:Tongue Neoplasms / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*
D016588:Anticarcinogenic Agents; D000975:Antioxidants; D002273:Carcinogens; D010936:Plant Extracts; D020011:Protective Agents; D005557:Formaldehyde; D015127:9,10-Dimethyl-1,2-benzanthracene; D007633:Keratins; C505409:Casp3 protein, mouse; D053148:Caspase 3
Mentha piperita; carcinogenesis induction; chemoprevention; dimethylbenz[a]anthracene; formaldehyde; mice tongues