Re-evaluation of binding properties of recombinant lymphocyte receptors NKR-P1A and CD69 to chemically synthesized glycans and peptides.
The binding of monosaccharides and short peptides to lymphocyte receptors (human CD69 and rat NKR-P1A) was first reported in 1994 and then in a number of subsequent publications. Based on this observation, numerous potentially high-affinity saccharide ligands have been synthesized over the last two decades in order to utilize their potential in antitumor therapy. Due to significant inconsistencies in their reported binding properties, we decided to re-examine the interaction between multiple ligands and CD69 or NKR-P1A. Using NMR titration and isothermal titration calorimetry we were unable to detect the binding of the tested ligands such as N-acetyl-D-hexosamines and oligopeptides to both receptors, which contradicts the previous observations published in more than twenty papers over the last fifteen years.
Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague 4 CZ14220, Czech Republic. rozbesky@gmail.com.
Daniel
D
Rozbeský
Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague 4 CZ14220, Czech Republic. hoficek@centrum.cz.
Jana
J
Krejzová
Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague 4 CZ14220, Czech Republic. krenek@biomed.cas.cz.
Karel
K
Křenek
Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague 4 CZ14220, Czech Republic. jan.prchal@vscht.cz.
Jan
J
Prchal
Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague 4 CZ14220, Czech Republic. richard.hrabal@vscht.cz.
Richard
R
Hrabal
Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague 4 CZ14220, Czech Republic. midlan@seznam.cz.
Milan
M
Kožíšek
Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague 4 CZ14220, Czech Republic. lenka.weignerova@gmail.com.
Lenka
L
Weignerová
Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague 4 CZ14220, Czech Republic. michele.fiore@ujf-grenoble.fr.
Michele
M
Fiore
Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague 4 CZ14220, Czech Republic. pascal.dumy@ujf-grenoble.fr.
Pascal
P
Dumy
Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague 4 CZ14220, Czech Republic. kren@biomed.cas.cz.
Vladimír
V
Křen
Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague 4 CZ14220, Czech Republic. ^olivier.renaudet@ujf-grenoble.fr.
Olivier
O
Renaudet
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism*; D006801:Humans; D037181:Lectins, C-Type / Q000378:metabolism*; D009842:Oligopeptides / Q000138:chemical synthesis / Q000494:pharmacology*; D011134:Polysaccharides / Q000138:chemical synthesis / Q000494:pharmacology*; D011485:Protein Binding; D051381:Rats; D011971:Receptors, Immunologic / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D037181:Lectins, C-Type; D009842:Oligopeptides; D011134:Polysaccharides; D011971:Receptors, Immunologic; D011994:Recombinant Proteins; D000099192:CD69 Antigens; C553558:Klrb1a protein, rat