[Study on the function of keratinocyte growth factor on apoptosis of oral mucosal epithelial cells].
OBJECTIVE
To study the function of keratinocyte growth factor (KGF) on apoptosis of oral mucosal epithelial cells and to provide a basis for further investigation of the role of KGF in the occurrence and development of oral mucosal diseases.


METHODS
Different concentrations of KGF (control group, 0 ng x mL(-1); experiment 1 group, 5 ng x mL(-1); experimental 2 group, 25 ng x mL(-1); experiment 3 group, 50 ng x mL(-1)) were added in oral mucosa epithelial cells cultured in vitro. After training for 12, 24, and 48 h, cell morphology was observed under an inverted microscope. Apoptosis was detected by using a flow cytometry instrument, and mRNA expression of apoptosis-related genes Bcl-2 and Bax was detected by using Real-Time fluorescent quantitative detection.


RESULTS
Cell adherence of the experimental group was more obvious than that of the control group, and the cell nucleolus of the experiment 3 group was obviously cultured at 48 h. After culturing for 48 h, the apoptosis rate and Bcl-2 and Bax mRNA expression among the four groups were statistically significant. The increase of KGF concentration, apoptosis rate, and Bax mRNA expression gradually reduced, whereas Bcl-2 mRNA expression increased (P < 0.05).


CONCLUSIONS
KGF may inhibit epithelial cell apoptosis through upregulation of Bcl-2 mRNA and downregulation of Bax mRNA.
Dept. of General Diagnosis, School of Stomatology, Shandong University, Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China.
Meirong
M
Wei
Dept. of General Diagnosis, School of Stomatology, Shandong University, Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China.
Guoju
G
Li
Dept. of Stomatology, Shandong Provincial Qianfoshan Hospital, Jinan 250015, China.
Da
D
Zhang
Dept. of General Diagnosis, School of Stomatology, Shandong University, Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China.
Shiguo
S
Yan
Dept. of General Diagnosis, School of Stomatology, Shandong University, Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China.
Xiangmin
X
Qi
D017209:Apoptosis; D004847:Epithelial Cells; D051523:Fibroblast Growth Factor 7; D006801:Humans; D009061:Mouth Mucosa; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger
C496453:FGF7 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051523:Fibroblast Growth Factor 7