Patterning gradients.
Surface-chemical and -morphological gradients can be extremely useful in cell-biological research as high-throughput screening tools-for example, exposing a given set of cells to many different surface conditions at once, under identical ambient conditions, in order to monitor cell behavior such as proliferation or specific gene expression. They can also be used to investigate the effects of gradients themselves on cell behavior, such as migration. A number of simple, reliable techniques for both chemical- and morphological-gradient fabrication have been developed in our laboratories and are described in detail in the following.
Laboratory for Surface Science and Technology, Department of Materials, ETH Zurich, Zurich, Switzerland.
Nagaiyanallur V
NV
Venkataraman
Laboratory for Surface Science and Technology, Department of Materials, ETH Zurich, Zurich, Switzerland.
Clément V M
CV
Cremmel
Laboratory for Surface Science and Technology, Department of Materials, ETH Zurich, Zurich, Switzerland; Phonak AG, Stäfa, Switzerland.
Christian
C
Zink
Laboratory for Surface Science and Technology, Department of Materials, ETH Zurich, Zurich, Switzerland.
Rebecca P
RP
Huber
Laboratory for Surface Science and Technology, Department of Materials, ETH Zurich, Zurich, Switzerland.
Nicholas D
ND
Spencer
D018929:Cell Culture Techniques / Q000379:methods*; D015870:Gene Expression; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D013499:Surface Properties
Cell migration; Gradient fabrication; High-throughput screening; Hydrophilicity; Hydrophobicity; Morphology gradient; Patterning; Roughness; Surface-chemical gradient