Oral Immunogenicity of plant-made Mycobacterium tuberculosis ESAT6 and CFP10.
Two lines of transgenic carrot plants producing Mycobacterium tuberculosis proteins (ESAT6 and CFP10) have been constructed. The target proteins are present in carrot storage roots at a level not less than 0.056% of the total storage protein (TSP) for ESAT6 and 0.002% of TSP for CFP10. As has been shown, oral immunization of mice induces both the cell-mediated and humoral immunities. These data suggest that the proteins in question are appropriate as a candidate edible vaccine against tuberculosis.
Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, pr. Lavrentieva 10, Novosibirsk 630090, Russia.
Elena A
EA
Uvarova
Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, pr. Lavrentieva 10, Novosibirsk 630090, Russia.
Pavel A
PA
Belavin
Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, pr. Lavrentieva 10, Novosibirsk 630090, Russia.
Natalya V
NV
Permyakova
Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, pr. Lavrentieva 10, Novosibirsk 630090, Russia.
Alla A
AA
Zagorskaya
Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, pr. Lavrentieva 10, Novosibirsk 630090, Russia ; State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk Oblast 630559, Russia.
Olesya V
OV
Nosareva
National Center of Biotechnology, ul. Valikhanova 13/1, Astana 010000, Kazakhstan.
Almagul A
AA
Kakimzhanova
Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, pr. Lavrentieva 10, Novosibirsk 630090, Russia.
Elena V
EV
Deineko
D000284:Administration, Oral; D000818:Animals; D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000235:genetics*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D018552:Daucus carota / Q000235:genetics; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000276:immunology; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000254:growth & development / Q000276:immunology; D014376:Tuberculosis / Q000276:immunology* / Q000517:prevention & control; D022462:Vaccines, Edible / Q000235:genetics
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C093276:ESAT-6 protein, Mycobacterium tuberculosis; D022462:Vaccines, Edible