Elevated serum IgE against MGL_1304 in patients with atopic dermatitis and cholinergic urticaria.
BACKGROUND
MGL_1304 secreted by Malassezia globosa is contained in human sweat and induces histamine release from basophils in patients with atopic dermatitis (AD) at a high positive rate. The aims of this study were to establish the enzyme-linked immunosorbent assay (ELISA) measuring specific immunoglobulins against MGL_1304 and to investigate the levels of these immunoglobulins in sera of patients with various allergic diseases.


METHODS
Purified MGL_1304 from human sweat (QRX) and recombinant MGL_1304 (rMGL_1304) were prepared for ELISA. To quantify the amount of MGL_1304-specific immunoglobulins, the standard serum was created by pooling sera of 20 patients with AD whose basophils released histamine in response to QRX. A monoclonal antibody which exhibited the highest neutralizing ability against QRX was established as Smith-2, and used as a capture antibody for the assay of QRX-specific IgE. A total of 156 subjects [normal controls (n = 23), AD (n = 63), cholinergic urticaria (CU) (n = 24), bronchial asthma (n = 32), and allergic rhinitis (n = 14)] were enrolled in this study.


RESULTS
ELISA methods to quantify the specific IgE, IgG and IgG4 against MGL_1304 in sera were successfully established. Levels of QRX-specific IgE in sera of patients with AD and CU were significantly higher than those of normal controls. Moreover, the levels of QRX-specific IgE and rMGL_1304-specific IgE in patients with AD were significantly correlated with their disease severities.


CONCLUSIONS
These ELISA methods to quantify the specific immunoglobulins against MGL_1304 are easy and useful means to assess allergy to MGL_1304. MGL_1304 contained in sweat is an important antigen for patients with AD and CU.
Department of Dermatology, Hiroshima University, Hiroshima, Japan.
Makiko
M
Hiragun
Department of Dermatology, Hiroshima University, Hiroshima, Japan.
Takaaki
T
Hiragun
Department of Dermatology, Hiroshima University, Hiroshima, Japan.
Kaori
K
Ishii
Department of Dermatology, Hiroshima University, Hiroshima, Japan; Faculty of Human Science, Hiroshima Bunkyo Women's University, Hiroshima, Japan.
Hidenori
H
Suzuki
Department of Dermatology, Hiroshima University, Hiroshima, Japan.
Akio
A
Tanaka
Department of Dermatology, Hiroshima University, Hiroshima, Japan.
Yuhki
Y
Yanase
Department of Dermatology, Hiroshima University, Hiroshima, Japan.
Shoji
S
Mihara
Department of Molecular and Internal Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
Yoshinori
Y
Haruta
Department of Molecular and Internal Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
Nobuoki
N
Kohno
Department of Dermatology, Hiroshima University, Hiroshima, Japan.
Michihiro
M
Hide
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000485:Allergens / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D001491:Basophils / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003876:Dermatitis, Atopic / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006636:Histamine Release; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D012720:Severity of Illness Index; D013542:Sweat / Q000276:immunology*; D014581:Urticaria / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D055815:Young Adult
D000485:Allergens; D007074:Immunoglobulin G; D007073:Immunoglobulin E