Heat-killed VSL#3 ameliorates dextran sulfate sodium (DSS)-induced acute experimental colitis in rats.
To determine the effects of heat-killed VSL#3 (B. breve, B. longum and B. infantis; L. plantarum, L. bulgaricus, L. casei and L. acidophilus; S. salivarius subsp. thermophilus) therapy in the dextran sulfate sodium (DSS)-induced acute experimental colitis in rats. Acute experimental colitis was induced in rats by 5% DSS and freely drink for seven days. Beginning on Day 8, rats underwent gavage once daily for seven days with heat-killed probiotic VSL#3 (0.6 g/kg/day), colonic damage was evaluated histologically and biochemically seven days after gavage. Expression of inflammatory related mediators (STAT3, P-STAT3) and cytokines (IL-6, IL-23, TGFβ) in colonic tissue were detected. The results revealed that heat-killed and live VSL#3 have identical anti-inflammatory properties by the assessed DAI (disease activity index), colon length, histological tissue and MPO activity. Heat-killed and live VSL#3 results in reduced IL-6, IL-23, TGFβ, STAT3 and P-STAT3 expression in colonic tissue. Heat-killed and live VSL#3 have showed the similar anti-inflammatory activity by inhibiting IL-6/STAT3 pathway in the DSS-induced acute experimental colitis in rats.
Department of Cadre Ward II, First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning, China. sanglixuan2008@163.com.
Li-Xuan
LX
Sang
Department of Gastroenterology, First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning, China. cb000216@163.com.
Bing
B
Chang
Department of Gastroenterology, First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning, China. congdai2006@sohu.com.
Cong
C
Dai
Department of Cadre Ward II, First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning, China. liyuchun522@163.com.
Nan
N
Gao
Department of Gastroenterology, First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning, China. weixinliu66@163.com.
Wei-Xin
WX
Liu
Department of Gastroenterology, First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning, China. fendou1957@163.com.
Min
M
Jiang
D000208:Acute Disease; D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000628:therapy*; D016264:Dextran Sulfate / Q000633:toxicity; D004195:Disease Models, Animal; D006358:Hot Temperature; D053759:Interleukin-23 / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D008297:Male; D019804:Mesalamine / Q000627:therapeutic use; D010766:Phosphorylation; D019936:Probiotics / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000378:metabolism; D012720:Severity of Illness Index; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism
D053759:Interleukin-23; D015850:Interleukin-6; D012333:RNA, Messenger; D050796:STAT3 Transcription Factor; D016212:Transforming Growth Factor beta; D019804:Mesalamine; D016264:Dextran Sulfate