Nitrite reductase genes as functional markers to investigate diversity of denitrifying bacteria during agricultural waste composting.
The purpose of this study was to investigate the diversity of denitrifier community during agricultural waste composting. The diversity and dynamics of the denitrifying genes (nirK and nirS) were determined using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Relationships between physico-chemical parameters and denitrifying genes structures were simultaneously evaluated by redundancy analysis (RDA). Phylogenetic analysis indicated that nirK clones grouped into six clusters and nirS clones into two major clusters, respectively. The results showed a very high diversity of nir gene sequences within composting samples. RDA showed that the nirK and nirS gene structures were significantly related to pH and pile temperature (P < 0.05). Significant amounts of the variation (49.2 and 38.3 % for nirK and nirS genes, respectively) were explained by pH and pile temperature, suggesting that those two parameters were the most likely ones to influence, or be influenced by the denitrifiers harboring nirK and nirS genes.
College of Environmental Science and Engineering, Hunan University, Changsha, 410082, People's Republic of China, cyn@hnu.edu.cn.
Yaoning
Y
Chen
Wei
W
Zhou
Yuanping
Y
Li
Jiachao
J
Zhang
Guangming
G
Zeng
Aizhi
A
Huang
Jingxia
J
Huang
D000383:Agriculture / Q000379:methods*; D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D044822:Biodiversity; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D058440:Denitrification; D006863:Hydrogen-Ion Concentration; D017744:Medical Waste Disposal / Q000379:methods*; D008969:Molecular Sequence Data; D009572:Nitrite Reductases / Q000032:analysis* / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012988:Soil Microbiology; D013696:Temperature
D004269:DNA, Bacterial; D017744:Medical Waste Disposal; D009572:Nitrite Reductases