Association of obesity with proteasomal gene polymorphisms in children.
The aim of this study was to ascertain possible associations between childhood obesity, its anthropometric and clinical parameters, and three loci of proteasomal genes rs2277460 (PSMA6 c.-110C>A), rs1048990 (PSMA6 c.-8C>G), and rs2348071 (PSMA3 c. 543+138G>A) implicated in obesity-related diseases. Obese subjects included 94 otherwise healthy children in Latvia. Loci were genotyped and then analyzed using polymerase chain reactions, with results compared to those of 191 nonobese controls. PSMA3 SNP frequency differences between obese children and controls, while not reaching significance, suggested a trend. These differences, however, proved highly significant (P < 0.002) in the subset of children reporting a family history of obesity. Among obese children denying such history, PSMA6 c.-8C>G SNP differences, while being nonsignificant, likewise suggested a trend in comparison to the nonobese controls. No PSMA6 c.-110C>A SNP differences were detected in the obese group or its subsets. Finally, PSMA3 SNP differences were significantly associated (P < 0.05) with circulating low-density lipoprotein cholesterol (LDL) levels. Our results clearly implicate the PSMA3 gene locus as an obesity risk factor in those Latvian children with a family history of obesity. While being speculative, the clinical results are suggestive of altered circulatory LDL levels playing a possible role in the etiology of obesity in the young.
Faculty of Medicine, University of Latvia, Sarlotes Street 1a, Riga 1001, Latvia ; Institute of Biology, University of Latvia, Miera Street 3, Salaspils 2169, Latvia ; Institute of Experimental and Clinical Medicine, University of Latvia, No. 4 Ojara Vaciesa Street, Riga 1004, Latvia.
Sarmite
S
Kupca
Institute of Biology, University of Latvia, Miera Street 3, Salaspils 2169, Latvia.
Tatjana
T
Sjakste
Institute of Biology, University of Latvia, Miera Street 3, Salaspils 2169, Latvia.
Natalija
N
Paramonova
Institute of Biology, University of Latvia, Miera Street 3, Salaspils 2169, Latvia.
Olga
O
Sugoka
Faculty of Medicine, University of Latvia, Sarlotes Street 1a, Riga 1001, Latvia.
Irena
I
Rinkuza
Faculty of Medicine, University of Latvia, Sarlotes Street 1a, Riga 1001, Latvia ; Institute of Biology, University of Latvia, Miera Street 3, Salaspils 2169, Latvia.
Ilva
I
Trapina
Faculty of Medicine, University of Latvia, Sarlotes Street 1a, Riga 1001, Latvia.
Ilva
I
Daugule
Faculty of Medicine, University of Latvia, Sarlotes Street 1a, Riga 1001, Latvia ; Institute of Experimental and Clinical Medicine, University of Latvia, No. 4 Ojara Vaciesa Street, Riga 1004, Latvia.
Alfred J
AJ
Sipols
Faculty of Medicine, University of Latvia, Sarlotes Street 1a, Riga 1001, Latvia.
Ingrida
I
Rumba-Rozenfelde
D000293:Adolescent; D000704:Analysis of Variance; D001786:Blood Glucose / Q000235:genetics; D001794:Blood Pressure / Q000235:genetics; D015992:Body Mass Index; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D008078:Cholesterol, LDL / Q000097:blood*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D063766:Pediatric Obesity / Q000150:complications / Q000453:epidemiology / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D046988:Proteasome Endopeptidase Complex / Q000235:genetics*
D001786:Blood Glucose; D008078:Cholesterol, LDL; C488165:PSMA3 protein, human; C465641:PSMA6 protein, human; D046988:Proteasome Endopeptidase Complex