Bone reconstruction in rat calvarial defects by chitosan/hydroxyapatite nanoparticles scaffold loaded with unrestricted somatic stem cells.
Calcium phosphates are one of the biomaterials that are used for bone regeneration. In this study, Hydroxyapatite (HAp) nanoparticles with chitosan gel filled with unrestricted somatic stem cells (USSCs) were used for healing calvarial bone in rat model. The healing effects of these injectable scaffolds with and without stem cells for bone regeneration were investigated by computed tomography (CT) analysis and pathology assays after 28 days of grafting. The results of CT analysis showing bone regeneration on the scaffolds, also the amounts of regenerated new bone for USSC scaffold was significantly greater than the scaffold without cell and untreated controls. Therefore, the combination of scaffold especially with USSC is considered as a useful method for bone regeneration.
Department of Biomedical Engineering, Islamic Azad University, Tonekabon Branch , Iran.
Esmaeil
E
Biazar
Saeed
S
Heidari Keshel
Mostafa Rezaei
MR
Tavirani
Rahim
R
Jahandideh
D053687:Adult Stem Cells / Q000637:transplantation*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D001861:Bone Regeneration / Q000187:drug effects*; D048271:Chitosan / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D005260:Female; D053758:Nanoparticles; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000166:cytology / Q000000981:diagnostic imaging / Q000502:physiology* / Q000601:surgery; D054457:Tissue Scaffolds / Q000737:chemistry*; D014057:Tomography, X-Ray Computed
D001672:Biocompatible Materials; D048271:Chitosan; D017886:Durapatite; D000469:Alkaline Phosphatase
bone regeneration; chitosan; hydroxyapatite nanoparticles; injectable scaffold; unrestricted somatic stem cells