Test-strip-based fluorometric detection of fluoride in aqueous media with a BODIPY-linked hydrogen-bonding receptor.
The measurement of biologically relevant anions, such as fluoride, is an important task in analytical chemistry, in particular, for dental health and osteoporosis. Although a large number of fluoride probes are known, the applicability under relevant conditions is limited to a few examples. To improve this situation, BODIPY-amidothiourea dyes with varying hydrogen-bond donating strengths were developed, the most H-acidic of which (1 c) could detect F(-) from an inorganic source (NaF) in 50 % aqueous solution (DMSO/water 1:1, v/v) with 0.01 ppm sensitivity through selective fluorescence quenching by a photoinduced electron-transfer (PET) process. Use of the probe and a reference dye with a test-strip assay and a portable and rapidly recording lateral-flow fluorescence reader made determination of F(-) in neat aqueous solutions, such as spiked water samples and toothpaste extracts, possible in a self-referenced manner, achieving a detection limit of 0.2 ppm.
BAM Bundesanstalt für Materialforschung und -prüfung Richard-Willstätter-Strasse 11, 12489 Berlin (Germany).
Pichandi
P
Ashokkumar
Hardy
H
Weißhoff
Werner
W
Kraus
Knut
K
Rurack
D001896:Boron Compounds / Q000737:chemistry*; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D005456:Fluorescent Dyes / Q000737:chemistry; D005459:Fluorides / Q000032:analysis*; D005470:Fluorometry; D006860:Hydrogen Bonding; D013890:Thiourea / Q000737:chemistry; D014867:Water / Q000737:chemistry
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D014867:Water; D013890:Thiourea; D005459:Fluorides
amidothiourea; fluorescent probes; fluoride; hydrogen bonds; photoinduced electron transfer