False positivity of gamma-glutamyl transpeptidase measurement in urine.
Although enzymuria tends to be associated to renal injury, there are no studies that have evaluated the presence of the enzyme gamma-glutamyl transpeptidase (GGT) spectrophotometry in the urine using a non-nephrotoxic agent (Nerium oleander) in order to evaluate the possibility of false positive results. The urinary GGT/urinary creatinine concentration ratio (uGGT/uCr) of 10 healthy dogs was calculated and posteriorly confronted with data from clinical evaluation, hematological and serum biochemical profiles, creatinine clearance (CrC), urinalysis, urine protein/creatinine ratio (UPC), electrocardiogram, systemic blood pressure (SBP) and light and electron microscopy. The results for kidney histology, SBP, UPC and CrC were not significantly different in any of the time-points analyzed. However, uGGT/uCr was significantly higher when measured 4 hours and 24 hours after administration of N. oleander. The measurement of the urinary GGT enzyme, as performed in many studies, yielded false positive results in dogs poisoned by a non-nephrotoxic agent.
Department of Veterinary Clinics and Surgery, São Paulo State University (UNESP) , Jaboticabal , Brazil .
Leandro Zuccolotto
LZ
Crivellenti
Javier Sousa
JS
Mesa
Adriana Érica Wilkes Burton
AÉ
Meirelles
Sofia
S
Borin Crivellenti
Edna Gomes
EG
Mireya
Julio Carlos
JC
Canola
Mário Roberto
MR
Hatayde
Aureo Evangelista
AE
Santana
Márcio
M
Dantas
Gyl Eanes Barros
GE
Silva
D058186:Acute Kidney Injury / Q000139:chemically induced / Q000201:enzymology* / Q000473:pathology*; D000818:Animals; D001794:Blood Pressure; D003404:Creatinine / Q000652:urine; D004285:Dogs; D004562:Electrocardiography; D005189:False Positive Reactions; D005260:Female; D007668:Kidney / Q000473:pathology*; D007677:Kidney Function Tests; D008297:Male; D030007:Nerium / Q000506:poisoning*; D011507:Proteinuria; D013053:Spectrophotometry; D005723:gamma-Glutamyltransferase / Q000652:urine*
D003404:Creatinine; D005723:gamma-Glutamyltransferase