Evaluation of menstrual blood stem cells seeded in biocompatible Bombyx mori silk fibroin scaffold for cardiac tissue engineering.
Recently, silk fibroin scaffolds have been introduced as novel and promising biomaterials in the field of cardiac tissue engineering. This study was designed to compare infiltration, proliferation, and cardiac differentiation potential of menstrual blood-derived stem cells (MenSCs) versus bone marrow-derived mesenchymal stem cells (BMSCs) in Bombyx mori-derived silk scaffold. Our primary data revealed that the fabricated scaffold has mechanical and physical qualities suitable for cardiac tissue engineering. The MenSCs tracking in scaffolds using immunofluorescent staining and scanning electron microscopy confirmed MenSCs attachment, penetration, and distribution within the porous scaffold matrix. Based on proliferation assay using propidium iodide DNA quantification, the significantly higher level of growth rates of both MenSCs and BMSCs was documented in scaffolds than that in two-dimensional culture (p < 0.01). The expression level of TNNT2, a bona fide cardiac differentiation marker, in BMSCs differentiated on silk scaffolds was markedly higher than those cultured in two-dimensional culture indicating the improvement of cardiac differentiation in the silk scaffolds. Furthermore, differentiated MenSCs exhibited higher expression of TNNT2 compared with induced BMSCs. It seems that silk scaffold-seeded MenSCs could be viewed as a novel, safe, natural, and accessible construct for cardiac tissue engineering.
Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
Maryam
M
Rahimi
Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
Homa
H
Mohseni-Kouchesfehani
Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran Immunology Research Center, Iran University of Medical Sciences, Tehran, Iran.
Amir-Hassan
AH
Zarnani
Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Sahba
S
Mobini
Reproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Shohreh
S
Nikoo
Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran s.kazemnejad@avicenna.ac.ir.
Somaieh
S
Kazemnejad
Menstrual blood; bone marrow; cardiac tissue engineering; silk scaffold; stem cells