Bioactive phthalides from Ligusticum sinense Oliv cv. Chaxiong.
Five new phthalides (1-4, 6), two new natural products (5, 7) and five known phthalides (8-12) were isolated from the aerial parts of Ligusticum sinense Oliv cv. Chaxiong. Their structures were elucidated by HR-ESI-MS, UV, IR, 1D and 2D NMR (HSQC, HMBC, (1)H-(1)H COSY, NOESY) methods. The absolute configurations were established by the circular dichroism (CD) spectrum and the modified Mosher's method. Compounds 1-8 were tested against SK-N-SH cell depriving oxygen and glucose and showed different degrees of increasing the cell survival, among which compounds 1, 4 and 8 (10 μM) showed higher cell survival than Ginsenoside Rg1.
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. Electronic address: weiqian1987615@163.com.
Qian
Q
Wei
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Jianbo
J
Yang
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Jin
J
Ren
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Aiguo
A
Wang
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Tengfei
T
Ji
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. Electronic address: suyalun@imm.ac.cn.
Yalun
Y
Su
D001572:Benzofurans / Q000737:chemistry / Q000302:isolation & purification*; D002460:Cell Line; D006801:Humans; D030004:Ligusticum / Q000737:chemistry*; D015394:Molecular Structure; D018696:Neuroprotective Agents / Q000737:chemistry / Q000302:isolation & purification*
D001572:Benzofurans; D018696:Neuroprotective Agents
Aerial parts; CD; Ligusticum sinense Oliv cv. Chaxiong; Modified Mosher's method; Neuronal impairment protective effect; Phthalide