The biocompatibility of silk fibroin and acellular collagen scaffolds for tissue engineering in the ear.
Recent experimental studies have shown the suitability of silk fibroin scaffold (SFS) and porcine-derived acellular collagen I/III scaffold (ACS) as onlay graft materials for tympanic membrane perforation repair. The aims of this study were to further characterize and evaluate the in vivo biocompatibility of SFS and ACS compared with commonly used materials such as Gelfoam and paper in a rat model. The scaffolds were implanted in subcutaneous (SC) tissue and middle ear (ME) cavity followed by histological and otoscopic evaluation for up to 26 weeks. Our results revealed that SFS and ACS were well tolerated and compatible in rat SC and ME tissues throughout the study. The tissue response adjacent to the implants evaluated by histology and otoscopy showed SFS and ACS to have a milder tissue response with minimal inflammation compared to that of paper. Gelfoam gave similar results to SFS and ACS after SC implantation, but it was found to be associated with pronounced fibrosis and osteoneogenesis after ME implantation. It is concluded that SFS and ACS both were biocompatible and could serve as potential alternative scaffolds for tissue engineering in the ear.
Ear Sciences Centre, School of Surgery, The University of Western Australia, Nedlands 6009, Western Australia, Australia. Ear Science Institute Australia, Subiaco 6008, Western Australia, Australia. Department of Otolaryngology, Head & Neck, Ningbo Lihuili Hospital (Ningbo Medical Centre), Ningbo 315041, Zhejiang, People's Republic of China.
Yi
Y
Shen
Sharon L
SL
Redmond
John M
JM
Papadimitriou
Bing M
BM
Teh
Sheng
S
Yan
Yan
Y
Wang
Marcus D
MD
Atlas
Robert J
RJ
Marano
Minghao
M
Zheng
Rodney J
RJ
Dilley
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D012831:Bombyx; D003094:Collagen / Q000737:chemistry*; D004423:Ear / Q000473:pathology*; D005349:Fibroins / Q000737:chemistry*; D005355:Fibrosis; D005782:Gels; D007150:Immunohistochemistry; D007249:Inflammation; D008297:Male; D010012:Osteogenesis; D038781:Otoscopy; D051381:Rats; D017207:Rats, Sprague-Dawley; D047011:Silk / Q000737:chemistry*; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014432:Tympanic Membrane / Q000473:pathology
D001672:Biocompatible Materials; D005782:Gels; D047011:Silk; D003094:Collagen; D005349:Fibroins