Influence of the λ780nm laser light on the repair of surgical bone defects grafted or not with biphasic synthetic micro-granular hydroxylapatite+Beta-Calcium triphosphate.
The treatment of bone loss due to different etiologic factors is difficult and many techniques aim to improve repair, including a wide range of biomaterials and, recently, photobioengineering. This work aimed to assess, through histological analysis The aim of this study was to assess, by light microscopy, the repair of bone defects grafted or not with biphasic synthetic micro-granular Calcium hydroxyapatite (HA)+Beta-TCP associated or not with Laser phototherapy - LPT (λ780nm). Forty rats were divided into 4 groups each subdivided into 2 subgroups according to the time of sacrifice (15 and 30days). Surgical bone defects were made on femur of each animal with a trephine drill. On animals of Clot group the defect was filled only by blood clot, on Laser group the defect filled with the clot was further irradiated. On animals of Biomaterial and Laser+Biomaterial groups the defect was filled by biomaterial and the last one was further irradiated (λ780nm, 70mW, spot size∼0.4cm(2), 20J/cm(2)-session, 140J/cm(2)-treatment) in four points around the defect at 48-h intervals and repeated for 2weeks. At both 15th and 30th days following sacrifice, samples were taken and analyzed by light microscopy. Many similarities were observed histologically between groups on regards bone reabsorption and neoformation, inflammatory infiltrate and collagen deposition. The criterion degree of maturation, marked by the presence of basophilic lines, indicated that the use of LPT associated with HA+Beta TCP graft, resulted in more advanced stage of bone repair at the end of the experiment.
Center of Biophotonics, School of Dentistry, Federal University of Bahia, Salvador, BA 40110-150, Brazil. Electronic address: luizguilherme_@hotmail.com.
Luiz Guilherme P
LG
Soares
Center of Biophotonics, School of Dentistry, Federal University of Bahia, Salvador, BA 40110-150, Brazil; National Institute of Optics and Photonics, University of São Paulo, Physics Institute of São Carlos, São Carlos, SP 13560-970, Brazil. Electronic address: cidamarques77@hotmail.com.
Aparecida Maria C
AM
Marques
Center of Biophotonics, School of Dentistry, Federal University of Bahia, Salvador, BA 40110-150, Brazil. Electronic address: mileguarda@hotmail.com.
Milena G
MG
Guarda
Center of Biophotonics, School of Dentistry, Federal University of Bahia, Salvador, BA 40110-150, Brazil. Electronic address: jouber_aciole@hotmail.com.
Jouber Mateus S
JM
Aciole
Laboratory of Surgical Pathology, School of Dentistry, Federal University of Bahia, Salvador, BA 40110-150, Brazil. Electronic address: jeanpatol@gmail.com.
Jean Nunes
JN
dos Santos
Center of Biophotonics, School of Dentistry, Federal University of Bahia, Salvador, BA 40110-150, Brazil; National Institute of Optics and Photonics, University of São Paulo, Physics Institute of São Carlos, São Carlos, SP 13560-970, Brazil; Camilo Castelo Branco University, Núcleo do Parque Tecnológico de São José dos Campos:Rod. Presidente Dutra Km 139, Eugênio de Melo, São José dos Campos, SP 12247-004, Brazil. Electronic address: albp@ufba.br.
Antonio Luiz B
AL
Pinheiro
D000818:Animals; D001672:Biocompatible Materials; D018786:Bone Substitutes / Q000627:therapeutic use; D001842:Bone and Bones / Q000473:pathology / Q000601:surgery*; D002130:Calcium Phosphates / Q000627:therapeutic use*; D017886:Durapatite / Q000627:therapeutic use*; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D017208:Rats, Wistar; D014945:Wound Healing
D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D017886:Durapatite
Biomaterial; Bone repair; Hydroxyapatite; Laser phototherapy