A D-amino acid containing peptide as a potent, noncovalent inhibitor of α5β1 integrin in human prostate cancer invasion and lung colonization.
Primary tumors often give rise to disseminated tumor cells (DTC's), which acquire full malignancy after invading distant site(s). Thus, DTC's may be a productive target for preventing prostate cancer metastasis progression. Our prior research showed that PHSCN peptide (Ac-PHSCN-NH2) targets activated α5β1 integrin to prevent invasion and metastasis in preclinical adenocarcinoma models, and disease progression in Phase I clinical trial. Here, we report that D-stereoisomer replacement of histidine and cysteine in PHSCN produces a highly potent derivative, Ac-PhScN-NH2 (PhScN). PhScN was 27,000- to 150,000-fold more potent as an inhibitor of basement membrane invasion by DU 145 and PC-3 prostate cancer cells. A large increase in invasion-inhibitory potency occurred after covalent modification of the sulfhydryl group in PHSCN to prevent disulfide bond formation; while the potency of covalently modified PhScN was not significantly increased. Thus PhScN and PHSCN invasion inhibition occurs by a noncovalent mechanism. These peptides also displayed similar cell surface binding dissociation constants (Kd), and competed for the same site. Consistent with its increased invasion-inhibitory potency, PhScN was also a highly potent inhibitor of lung extravasation and colonization in athymic nude mice: it was several hundred- or several thousand-fold more potent than PHSCN at blocking extravasation by PC-3 or DU 145 cells, and 111,000- or 379,000-fold more potent at inhibiting lung colonization, respectively. Furthermore, systemic 5 mg/kg PhScN monotherapy was sufficient to cause complete regression of established, intramuscular DU 145 tumors. PhScN thus represents a potent new family of therapeutic agents targeting metastasis by DTC's to prevent parallel progression in prostate cancer.
Department of Radiation Oncology, University of Michigan, Room 4424F Medical Science 1, 1301 Catherine Street, Ann Arbor, MI, 48109-5637, USA.
Donna M
DM
Veine
Department of Radiation Oncology, University of Michigan, Room 4424F Medical Science 1, 1301 Catherine Street, Ann Arbor, MI, 48109-5637, USA.
Hongren
H
Yao
Department of Radiation Oncology, University of Michigan, Room 4424F Medical Science 1, 1301 Catherine Street, Ann Arbor, MI, 48109-5637, USA.
Daniel R
DR
Stafford
Department of Radiation Oncology, University of Michigan, Room 4424F Medical Science 1, 1301 Catherine Street, Ann Arbor, MI, 48109-5637, USA.
Kevin S
KS
Fay
Department of Radiation Oncology, University of Michigan, Room 4424F Medical Science 1, 1301 Catherine Street, Ann Arbor, MI, 48109-5637, USA. dlivant@umich.edu.
Donna L
DL
Livant
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D005260:Female; D006801:Humans; D039081:Integrin alpha5beta1 / Q000037:antagonists & inhibitors*; D008175:Lung Neoplasms / Q000378:metabolism / Q000517:prevention & control* / Q000556:secondary; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D010446:Peptide Fragments / Q000494:pharmacology*; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
D000596:Amino Acids; D039081:Integrin alpha5beta1; D010446:Peptide Fragments
Alpha5 beta1 integrin; D-amino acids; Disseminated tumor cells; Extravasation; Invasion; Lung colonization