Upregulation of TFAM and mitochondria copy number in human lymphoblastoid cells.
Mitochondria are central to several physiological and pathological conditions in humans. In the present study, we performed copy number analysis of nuclear encoded mitochondrial genes, in peripheral blood mononuclear cells (PBMCs) and its representative lymphoblastoid cells (LCLs). We have observed hyper diploid copies of mitochondrial transcription factor A (TFAM) gene in the LCLs along with increased mtDNA copy number, mitochondrial mass, intracellular ROS and mitochondrial membrane potential, suggesting elevated mitochondrial biogenesis in LCLs. Gene expression analysis confirmed TFAM over-expression in LCLs when compared to PBMC. Based on our observation, we suggest that increased copy number of TFAM gene upregulates its expression, increases mtDNA copy numbers and protects it from oxidative stress induced damage in the transformed LCLs.
Division of Biotechnology, Manipal Life Sciences Centre, Manipal University, Manipal 576104, Karnataka State, India. Electronic address: sanjiban.c@gmail.com.
Sanjiban
S
Chakrabarty
Division of Biotechnology, Manipal Life Sciences Centre, Manipal University, Manipal 576104, Karnataka State, India. Electronic address: reshdsouza@gmail.com.
Reena Reshma
RR
D'Souza
Division of Biotechnology, Manipal Life Sciences Centre, Manipal University, Manipal 576104, Karnataka State, India. Electronic address: spbhat81@gmail.com.
Shama Prasada
SP
Kabekkodu
Division of Biotechnology, Manipal Life Sciences Centre, Manipal University, Manipal 576104, Karnataka State, India. Electronic address: gopinathpm@yahoo.com.
Puthiya M
PM
Gopinath
Institut National de la Santé et de la Recherché Médicale (INSERM), U688 Physiopathologie Mitochondriale, Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux cedex, France. Electronic address: rossig@u-bordeaux2.fr.
Rodrigue
R
Rossignol
Division of Biotechnology, Manipal Life Sciences Centre, Manipal University, Manipal 576104, Karnataka State, India. Electronic address: ksatyamoorthy@manipal.edu.
Kapaettu
K
Satyamoorthy
D002454:Cell Differentiation; D002478:Cells, Cultured; D004272:DNA, Mitochondrial / Q000032:analysis / Q000235:genetics; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000235:genetics; D018628:Gene Dosage; D020869:Gene Expression Profiling; D006801:Humans; D008214:Lymphocytes / Q000502:physiology*; D008928:Mitochondria / Q000235:genetics / Q000502:physiology*; D024101:Mitochondrial Proteins / Q000032:analysis* / Q000235:genetics; D017382:Reactive Oxygen Species / Q000032:analysis; D014157:Transcription Factors / Q000032:analysis* / Q000235:genetics; D015854:Up-Regulation
D004272:DNA, Mitochondrial; D004268:DNA-Binding Proteins; D024101:Mitochondrial Proteins; D017382:Reactive Oxygen Species; C499631:TFAM protein, human; D014157:Transcription Factors
Copy number; Lymphoblastoid cell lines; Mitochondria; TFAM