Isolation and identification of hexazinone-degrading bacterium from sugarcane-cultivated soil in Kenya.
The s-triazine herbicide hexazinone [3-cyclohexyl-6-dimethylamino-1-methyl-1,3,5-triazine-2,4(1H,3H)-dione], is widely used in agriculture for weed control. Laboratory biodegradation experiments for hexazinone in liquid cultures were carried out using sugarcane-cultivated soils in Kenya. Liquid culture experiments with hexazinone as the only carbon source led to the isolation of a bacterial strain capable of its degradation. Through morphological, biochemical and molecular characterization by 16S rRNA, the isolate was identified as Enterobacter cloacae. The isolate degraded hexazinone up to 27.3% of the initially applied concentration of 40 μg mL(-1) after 37 days of incubation in a liquid culture medium. The study reports the degradation of hexazinone and characterization of the isolated bacterial strain.
Department of Physical Sciences, Multimedia University of Kenya, P.O. Box 30305-00100, Magadi Road, Nairobi, Kenya, anjokingugi@yahoo.com.
Anastasiah
A
Ngigi
Zachary
Z
Getenga
Hamadi
H
Boga
Paul
P
Ndalut
D000383:Agriculture; D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D001673:Biodegradation, Environmental; D006540:Herbicides / Q000032:analysis / Q000378:metabolism*; D007630:Kenya; D031786:Saccharum; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D014227:Triazines / Q000032:analysis / Q000378:metabolism*
D006540:Herbicides; D012987:Soil; D014227:Triazines; C025641:hexazinone