DNA assembly of nanoparticle superstructures for controlled biological delivery and elimination.
The assembly of nanomaterials using DNA can produce complex nanostructures, but the biological applications of these structures remain unexplored. Here, we describe the use of DNA to control the biological delivery and elimination of inorganic nanoparticles by organizing them into colloidal superstructures. The individual nanoparticles serve as building blocks, whose size, surface chemistry and assembly architecture dictate the overall superstructure design. These superstructures interact with cells and tissues as a function of their design, but subsequently degrade into building blocks that can escape biological sequestration. We demonstrate that this strategy reduces nanoparticle retention by macrophages and improves their in vivo tumour accumulation and whole-body elimination. Superstructures can be further functionalized to carry and protect imaging or therapeutic agents against enzymatic degradation. These results suggest a different strategy to engineer nanostructure interactions with biological systems and highlight new directions in the design of biodegradable and multifunctional nanomedicine.
1] Institute of Biomaterials and Biomedical Engineering, Rosebrugh Building, Room 407, 164 College Street, Toronto, Ontario M5S 3G9, Canada [2].
Leo Y T
LY
Chou
1] Institute of Biomaterials and Biomedical Engineering, Rosebrugh Building, Room 407, 164 College Street, Toronto, Ontario M5S 3G9, Canada [2].
Kyryl
K
Zagorovsky
1] Institute of Biomaterials and Biomedical Engineering, Rosebrugh Building, Room 407, 164 College Street, Toronto, Ontario M5S 3G9, Canada [2] Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Room 230, Toronto, Ontario M5S 3E1, Canada [3] Department of Chemical Engineering, 200 College Street, Toronto, Ontario M5S 3E5, Canada [4] Department of Chemistry, 80 St George Street, Toronto, Ontario M5S 3H6, Canada [5] Department of Material Science and Engineering, 160 College Street, Room 450, University of Toronto, Toronto M5S 3E1, Canada.
Warren C W
WC
Chan
D000818:Animals; D004247:DNA / Q000737:chemistry* / Q000378:metabolism / Q000627:therapeutic use; D016503:Drug Delivery Systems; D005089:Exocytosis / Q000502:physiology; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D050997:Nanomedicine / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use; D049329:Nanostructures / Q000737:chemistry* / Q000627:therapeutic use; D009369:Neoplasms / Q000737:chemistry / Q000188:drug therapy / Q000378:metabolism
D004247:DNA