Prevalence of human papillomavirus infection in the oropharynx and urine among sexually active men: a comparative study of infection by papillomavirus and other organisms, including Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma spp., and Ureaplasma spp.
BACKGROUND
Oropharyngeal squamous cell carcinoma (OSCC) has shown a gradual increase in male predominance due to the increasing incidence of human papillomavirus (HPV)-associated OSCC. However, the mode of HPV transmission to the oral cavity is poorly understood, and little is known about the epidemiology of oral HPV infection in men. The prevalence rates of HPV, Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma spp., and Ureaplasma spp. were compared in the oropharynx (oral cavity) and urine of male Japanese patients attending a sexually transmitted disease clinic.


METHODS
The study population consisted of 213 men aged 16 - 70 years old (mean: 34.4 years old). Oropharyngeal gargles and urine were collected, and sedimented cells were preserved in liquid-based cytology solution. After DNA extraction, β-globin and infectious organisms were analyzed by a PCR-based method. The HPV genotype was determined by HPV GenoArray test.


RESULTS
β-Globin was positive in 100% and 97.7% of oral and urine samples, respectively. HPV detection rates were 18.8% and 22.1% in oral and urine samples, respectively, suggesting that the prevalence of HPV infection in the oral cavity was similar to that in the urinary tract. N. gonorrhoeae was more prevalent in oral (15.6%) than urine samples (9.1%), whereas C. trachomatis was detected more frequently in urine (15.9%) than oral samples (4.2%). The detection rates of M. genitalium, M. hominis, and Ureaplasma spp. were 5.2%, 10.3%, and 16.0% in oral samples, and 7.7%, 6.3%, and 19.2% in urine, respectively. There were no significant differences in the detection rates of Mycoplasma spp. and Ureaplasma spp. between anatomical locations. The distribution of HPV types were similar in oral and urine samples, and HPV16 was the most common type. The majority of men with HPV infection in both the oral cavity and urine had concordant oral and urinary HPV infection. The presence of urinary HPV infection was an independent risk factor of oral HPV infection, with an odds ratio of 3.39 (95% CI: 1.49 - 7.71), whereas oral gonococcal infection was inversely correlated with oral HPV infection (odds ratio: 0.096; 95% CI: 0.01 - 0.77).


CONCLUSIONS
Oral HPV infection commonly occurs in sexually active men, and is significantly correlated with urinary HPV infection.
Kazufumi
K
Nakashima
Department of Integrative Cancer Therapy and Urology, Kanazawa University Graduate School of Medical Science, 13-1, Takaramachi, Kanazawa, Ishikawa 920-8641, Japan. kshigehara0415@yahoo.co.jp.
Kazuyoshi
K
Shigehara
Shohei
S
Kawaguchi
Akira
A
Wakatsuki
Yoshitomo
Y
Kobori
Kazuyoshi
K
Nakashima
Yasunori
Y
Ishii
Masayoshi
M
Shimamura
Toshiyuki
T
Sasagawa
Yasuhide
Y
Kitagawa
Atsushi
A
Mizokami
Mikio
M
Namiki
D000293:Adolescent; D000328:Adult; D000368:Aged; D002690:Chlamydia Infections / Q000453:epidemiology; D002692:Chlamydia trachomatis / Q000302:isolation & purification; D003141:Communicable Diseases; D006069:Gonorrhea / Q000453:epidemiology; D052162:Human papillomavirus 16 / Q000302:isolation & purification; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D009174:Mycoplasma / Q000302:isolation & purification; D009175:Mycoplasma Infections / Q000453:epidemiology; D009344:Neisseria gonorrhoeae / Q000302:isolation & purification; D009960:Oropharynx / Q000821:virology*; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000652:urine; D015995:Prevalence; D012141:Respiratory Tract Infections / Q000453:epidemiology* / Q000652:urine / Q000821:virology; D015229:Sexually Transmitted Diseases, Viral / Q000453:epidemiology* / Q000652:urine / Q000821:virology; D014509:Ureaplasma / Q000302:isolation & purification; D016869:Ureaplasma Infections / Q000453:epidemiology; D055815:Young Adult