The response of mouse embryonic stem cells to low doses of γ-radiation: evidence for an adaptive response.
Low doses of ionizing radiation may induce an adaptive mechanism which protects embryonic stem cells against higher doses, a phenomenon which was reported previously for somatic cells. In this study, a possible adaptive response (AR) was evaluated by measuring cell survival (MTT assay) and chromosomal aberrations (micronucleus assay). Thymidine-synchronized mouse embryonic stem cells (mESCs) were exposed to 2.5, 3.7, or 5cGy (60)Co γ-rays and, after 5h challenged by a dose of 150cGy. mESCs pre-irradiated at 2.5cGy showed an adaptive response.
Department of Genetics at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. Electronic address: kalantary.hamid@gmail.com.
Hamid
H
Kalantari
Department of Biology, Faculty of Science, University of Tehran, Iran.
Nasrin
N
Motamed
Department of Genetics at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
Anahita
A
Mohseni Meybodi
Shahid Beheshti Medical Sciences University, Shohada Hospital, Radiation Oncology Department, Tehran, Iran.
Ali
A
Jabbari Arfaie
Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
Hossein
H
Baharvand
Department of Genetics at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. Electronic address: gourabi@royaninstitute.org.
Hamid
H
Gourabi
D000220:Adaptation, Biological / Q000528:radiation effects; D000818:Animals; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D053595:Embryonic Stem Cells / Q000502:physiology / Q000528:radiation effects*; D005720:Gamma Rays / Q000009:adverse effects*; D051379:Mice; D015162:Micronucleus Tests; D011829:Radiation Dosage
Adaptive response; Cytochalasin-B; Ionizing radiation; MTT assay; Micronucleus assay; Mouse embryonic stem cell