[Characteristics of toxigenic properties of Myrothecium cinctum (Corda) sacc., 1886 and Myrothecium commune pidopl., 1969].
The comparative study of toxigenic potential of Myrothecium cinctum (12 strains) and Myrothecium commune (7 strains) was conducted. The ability to produce the complex of macrocyclic trichothecenes (MCTC) presented in ethanol soluble fraction (ESF), as well as fungistatic activity against yeasts test-cultures (strains Saccharomyces vini "Feodosia" and Candida stellatoidea 62) and rabbit skin test were evaluated. It was established that ESF output varied within wide limits in all studied strains of both species but it was significantly lower in M. commune strains. Higher ESF yields were accompanied as a rule by higher fungistatic activity and skin reaction in rabbits. The separate strains showed high biological activity with minor ESF output that could be possible caused by domination of more toxic components in complex preparation. The study of MCTC component structure by high performance liquid chromatography method (HPLC) showed with a few exceptions the presence of verrucarins A and J, roridins A, E, and H as well as unidentified components whose column retention times were 2.1 and 4.9 minutes. The production of roridin H and unknown component with 2.1 min. retention time was typical of M. cinctum strains while verrucarin A and unidentified component with 4.9 min. retention time was typical of M. commune strains.
A M
AM
Zaĭchenko
E S
ES
Tsyganenko
E V
EV
Andrienko
Ia I
IaI
Savchuk
D000818:Animals; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D002175:Candida / Q000187:drug effects* / Q000254:growth & development; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol; D006999:Hypocreales / Q000737:chemistry*; D059627:Liquid Phase Microextraction; D011817:Rabbits; D012440:Saccharomyces / Q000187:drug effects* / Q000254:growth & development; D012867:Skin / Q000187:drug effects; D012882:Skin Tests; D013045:Species Specificity; D014255:Trichothecenes / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*
D000935:Antifungal Agents; D014255:Trichothecenes; D000431:Ethanol