Vaccination with recombinant Brugia malayi cystatin proteins alters worm migration, homing and final niche selection following a subcutaneous challenge of Mongolian gerbils (Meriones unguiculatus) with B. malayi infective larvae.
BACKGROUND
Cysteine protease inhibitors of Brugia malayi have been ascribed to be involved in parasite development as well as to immunomodulate the host's immune response. In Onchocerca volvulus, Onchocystatin has been shown to induce partial protection in the mouse diffusion chamber vaccination model. In the present study we investigated the impact of vaccination with recombinant Bm-CPI-1 and Bm-CPI-2 proteins on protection against a subcutaneous challenge of B. malayi third stage larvae in gerbils.


RESULTS
Vaccination with E. coli derived recombinant B. malayi cysteine protease inhibitors (Bm-CPI-1 or -2) did not confer protection against B. malayi L3 challenge infection in gerbils but altered the homing of a significant number of adult worms from the lymphatics to the heart and lungs.


CONCLUSIONS
Bm-CPI vaccination-induced alteration in worm migration is consistent with our previous observations in gerbils vaccinated with B. pahangi excretory-secretory (ES) proteins, which resulted in delayed migration of the L3s and altered the final location of adult worms. Similar observations have also been made in dogs vaccinated with Ancylostoma caninum proteins; an increased number of worms were recovered in the colon and not the expected small intestine. A change in the final niche was also reported in immune versus non-immune hosts of two other gut dwelling nematodes. Vaccination induced alteration of the parasite's final homing might be a rare or a common phenomenon, which unfortunately is rarely recorded. The reason for the alteration in the final niche selection by adult nematode worms following vaccination is unknown and necessitates further investigation.
Sridhar
S
Arumugam
Bin
B
Zhan
David
D
Abraham
Danielle
D
Ward
Sara
S
Lustigman
Department of Pathobiological Sciences, LSU School of Veterinary Medicine, Louisiana State University, 1909 Skip Bertman Drive, Baton Rouge, LA 70803, USA. tklei1@lsu.edu.
Thomas R
TR
Klei
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D017178:Brugia malayi / Q000254:growth & development / Q000276:immunology*; D015891:Cystatins / Q000276:immunology*; D005368:Filariasis / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control*; D005849:Gerbillinae; D015801:Helminth Proteins / Q000276:immunology*; D007814:Larva; D008297:Male; D011994:Recombinant Proteins / Q000276:immunology; D014611:Vaccination
D000909:Antibodies, Helminth; D015891:Cystatins; D015801:Helminth Proteins; D011994:Recombinant Proteins