Preparation of molecular imprinted microspheres based on inorganic-organic co-functional monomer for miniaturized solid-phase extraction of fluoroquinolones in milk.
An inorganic-organic co-functional monomer, methacrylic acid-vinyltriethoxysilan (MAA-VTES) was designed for the synthesis of molecularly imprinted microspheres (MIMs). By virtue of the aqueous suspension polymerization and dummy template (pazufloxacin), the obtained MAA-VTES based MIMs exhibited good recognition and selectivity to fluoroquinolones (FQs), and were successfully applied as selective sorbents of a miniaturized home-made solid phase extraction device for the determination of ofloxacin (OFL), lomefloxacin (LOM) and ciprofloxacin (CIP) in milk samples. Under the optimum conditions of the miniaturized molecularly imprinted solid phase extraction (mini-MISPE) coupled with liquid chromatography-ultraviolet detector (LC-UV), good linearities were obtained for three FQs in a range of 0.2-20.0μgmL(-1) and the average recoveries at three spiked levels were ranged from 87.2% to 106.1% with the relative standard deviation (RSD) less than 5.4%. The presented co-functional monomer based mini-MISPE-LC-UV protocol introduced the rigidity and flexibility of inorganic silicon materials, exhibited excellent extraction performance towards targets, and could be potentially applied to the determination of FQs in milk samples.
Department of Pharmacology, Xingtai Medical College, Xingtai 054000, China. Electronic address: hzlx87@163.com.
Hui
H
Wang
Department of Pharmacy, Shijiazhuang Maternal and Child Health Hospital, Shijiazhuang 050080, China.
Ruiling
R
Wang
College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China.
Yehong
Y
Han
D000818:Animals; D004350:Drug Residues / Q000032:analysis* / Q000737:chemistry; D024841:Fluoroquinolones / Q000032:analysis* / Q000737:chemistry; D008863:Microspheres; D008892:Milk / Q000737:chemistry*; D054802:Molecular Imprinting / Q000379:methods*; D052616:Solid Phase Extraction / Q000379:methods*
D024841:Fluoroquinolones
Fluoroquinolones; Home-made solid-phase extraction; Inorganic–organic co-functional monomer; Milk samples; Molecularly imprinted microspheres