The Plasmodium falciparum translationally controlled tumor protein (TCTP) is incorporated more efficiently into B cells than its human homologue.
Plasmodium falciparum secretes a homologue of the translationally controlled tumor protein (TCTP) into serum of infected individuals, although its role in pathogenesis or virulence is unknown. To determine the effect of P. falciparum TCTP on B cells as compared to human TCTP, fluorescently labeled proteins were incubated on primary cultures of mouse splenic B cells and analyzed by flow cytometry and confocal microscopy. Our results indicate that both recombinant proteins are incorporated into B cells, but differ significantly in their rate and percentage of incorporation, being significantly higher for P. falciparum TCTP. Furthermore, P. falciparum TCTP showed a lower B cell proliferative effect than human TCTP, suggesting a mechanism through which the former could interfere in the host's immune response.
Department of Biotechnology and Bioengineering CINVESTAV-IPN, Mexico City, Mexico.
Berenice
B
Calderón-Pérez
Department of Biotechnology and Bioengineering CINVESTAV-IPN, Mexico City, Mexico.
Beatriz
B
Xoconostle-Cázares
Unidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, CMN Siglo XXI IMSS, México City, Mexico.
Rosalía
R
Lira-Carmona
Department of Molecular Biomedicine, CINVESTAV-IPN, Mexico City, Mexico.
Rosaura
R
Hernández-Rivas
Department of Biotechnology and Bioengineering CINVESTAV-IPN, Mexico City, Mexico.
Jaime
J
Ortega-López
Department of Biotechnology and Bioengineering CINVESTAV-IPN, Mexico City, Mexico.
Roberto
R
Ruiz-Medrano
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010963:Plasmodium falciparum / Q000378:metabolism*; D021381:Protein Transport / Q000187:drug effects; D015800:Protozoan Proteins / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000166:cytology; D000090324:Tumor Protein, Translationally-Controlled 1
D014408:Biomarkers, Tumor; D005456:Fluorescent Dyes; D015800:Protozoan Proteins; D011994:Recombinant Proteins; C000715647:TPT1 protein, human; C000715648:Tpt1 protein, mouse; D000090324:Tumor Protein, Translationally-Controlled 1