Modeling pure culture heterotrophic production of polyhydroxybutyrate (PHB).
In this contribution a mechanistic model describing the production of polyhydroxybutyrate (PHB) through pure-culture fermentation was developed, calibrated and validated for two different substrates, namely glucose and waste glycerol. In both cases, non-growth-associated PHB production was triggered by applying nitrogen limitation. The occurrence of some growth-associated PHB production besides non-growth-associated PHB production was demonstrated, although it is inhibited in the presence of nitrogen. Other phenomena observed experimentally and described by the model included biomass growth on PHB and non-linear product inhibition of PHB production. The accumulated impurities from the waste substrate negatively affected the obtained maximum PHB content. Overall, the developed mathematical model provided an accurate prediction of the dynamic behavior of heterotrophic biomass growth and PHB production in a two-phase pure culture system.
Flemish Institute for Technological Research (VITO), Business Unit Separation and Conversion Technology, Boeretang 200, 2400 Mol, Belgium; Ghent University, Department of Biosystems Engineering, Coupure Links 653, 9000 Gent, Belgium. Electronic address: salatulislam.mozumder@vito.be.
Md Salatul Islam
MS
Mozumder
Ghent University, Department of Biosystems Engineering, Coupure Links 653, 9000 Gent, Belgium.
Laurens
L
Goormachtigh
Flemish Institute for Technological Research (VITO), Business Unit Separation and Conversion Technology, Boeretang 200, 2400 Mol, Belgium.
Linsey
L
Garcia-Gonzalez
Flemish Institute for Technological Research (VITO), Business Unit Separation and Conversion Technology, Boeretang 200, 2400 Mol, Belgium.
Heleen
H
De Wever
Ghent University, Department of Biosystems Engineering, Coupure Links 653, 9000 Gent, Belgium.
Eveline I P
EI
Volcke
D018533:Biomass; D019149:Bioreactors; D053898:Biosynthetic Pathways / Q000502:physiology*; D003198:Computer Simulation; D020118:Cupriavidus necator / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000032:analysis; D005990:Glycerol / Q000032:analysis; D006885:Hydroxybutyrates / Q000378:metabolism*; D007700:Kinetics; D008954:Models, Biological; D011091:Polyesters / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
D005947:Glucose; D005990:Glycerol; D006885:Hydroxybutyrates; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates
Cell density inhibition; Fermentation; Polyhydroxybutyrate (PHB); Product inhibition; Simulation