[Gene detection of GPD1-L and the association with sudden unexplained death syndrome in young adults].
OBJECTIVE
To analyze the variations of glycerol-3-phosphate dehydrogenase 1 like gene (GPD1-L) and address the association with sudden manhood death syndrome (SMDS).


METHODS
The genomic DNA was extracted from blood samples of the SMDS group and the normal control group. The exons, exon-intron boundaries and 3'-UTRs of coding region of GPD1-L were PCR amplified and DNA sequenced directly to confirm the types of variations. The genotype frequency and allele frequency were analyzed statistically.


RESULTS
There were two variants in the SMDS group, c.465C>T and c.*18G>T, the latter existed certain degree difference of genotype distribution and allele frequency between the SMDS group and the control group, but there was no statistically significant (P > 0.05).


CONCLUSIONS
The relation between gene mutation of GPD1-L and the occurrence of Chinese SMDS deserves a further research.
Pingshan Branch of Shenzhen Public Security Bureau, Shenzhen 518118, China. 992081468@qq.com
Xiao-long
XL
Xu
Luohu Branch of Shenzhen Public Security Bureau, Shenzhen 518004, China.
Wen
W
Wang
Guangzhou Institute of Criminal Science and Technology, Guangzhou 510030, China.
Chao
C
Liu
Department of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, China.
Yi-ding
YD
Hou
Department of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, China.
Lei
L
Huang
Guangzhou Institute of Criminal Science and Technology, Guangzhou 510030, China.
Chang-hui
CH
Liu
Guangzhou Institute of Criminal Science and Technology, Guangzhou 510030, China.
Yue
Y
Li
Department of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, China.
Jian-ding
JD
Cheng
D000293:Adolescent; D000328:Adult; D044466:Asian People / Q000235:genetics; D001483:Base Sequence; D016022:Case-Control Studies; D004252:DNA Mutational Analysis; D017931:DNA Primers / Q000235:genetics; D003645:Death, Sudden / Q000209:etiology*; D005091:Exons; D005787:Gene Frequency; D005838:Genotype; D005993:Glycerolphosphate Dehydrogenase / Q000097:blood / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D055815:Young Adult
D017931:DNA Primers; C525035:GPD1L protein, human; D005993:Glycerolphosphate Dehydrogenase