Treatment with pyranopyran-1, 8-dione attenuates airway responses in cockroach allergen sensitized asthma in mice.
Chronic allergic asthma is characterized by Th2-typed inflammation, and contributes to airway remodeling and the deterioration of lung function. Viticis Fructus (VF) has long been used in China and Korea as a traditional herbal remedy for treating various inflammatory diseases. Previously, we have isolated a novel phytochemical, pyranopyran-1, 8-dione (PPY), from VF. This study was conducted to evaluate the ability of PPY to prevent airway inflammation and to attenuate airway responses in a cockroach allergen-induced asthma model in mice. The mice sensitized to and challenged with cockroach allergen were treated with oral administration of PPY. The infiltration of total cells, eosinophils and lymphocytes into the BAL fluid was significantly inhibited in cockroach allergen-induced asthma mice treated with PPY (1, 2, or 10 mg/kg). Th2 cytokines and chemokine, such as IL-4, IL-5, IL-13 and eotaxin in BAL fluid were also reduced to normal levels following treatment with PPY. In addition, the levels of IgE were also markedly suppressed after PPY treatment. Histopathological examination demonstrated that PPY substantially inhibited eosinophil infiltration into the airway, goblet cell hyperplasia and smooth muscle hypertrophy. Taken together, these results demonstrate that PPY possesses a potent efficacy on controlling allergic asthma response such as airway inflammation and remodeling.
College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea.
Soojin
S
Park
Department of East-West Medicine, Kyung Hee University, Seoul, Republic of Korea.
Min-Sun
MS
Park
College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea.
Kyung-Hwa
KH
Jung
College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea.
Joohyun
J
Song
Natural Products Department, Korea Promotion Institute for Traditional Medical Industry, Kyungbuk, Republic of Korea.
You Ah
YA
Kim
Natural Products Department, Korea Promotion Institute for Traditional Medical Industry, Kyungbuk, Republic of Korea.
Hi Jae
HJ
Cho
Department of East-West Medicine, Kyung Hee University, Seoul, Republic of Korea.
Byung-Il
BI
Min
College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea.
Hyunsu
H
Bae
D000284:Administration, Oral; D056151:Airway Remodeling / Q000187:drug effects; D000485:Allergens / Q000276:immunology; D000818:Animals; D018927:Anti-Asthmatic Agents / Q000008:administration & dosage*; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D001249:Asthma / Q000188:drug therapy* / Q000276:immunology; D003058:Cockroaches / Q000276:immunology; D016207:Cytokines / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D008168:Lung / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011753:Pyrones / Q000008:administration & dosage*; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism
C545170:1H,8H-pyrano(3,4-c)pyran-1,8-dione; D000485:Allergens; D018927:Anti-Asthmatic Agents; D000893:Anti-Inflammatory Agents; D016207:Cytokines; D011753:Pyrones