Isolation and transplantation of corneal endothelial cell-like cells derived from in-vitro-differentiated human embryonic stem cells.
The maintenance of corneal dehydration and transparency depends on barrier and pump functions of corneal endothelial cells (CECs). The human CECs have no proliferation capacity in vivo and the ability to divide in vitro under culture conditions is dramatically limited. Thus, the acquisition of massive cells analogous to normal human CECs is extremely necessary whether from the perspective of cellular basic research or from clinical applications. Here we report the derivation of CEC-like cells from human embryonic stem cells (hESCs) through the periocular mesenchymal precursor (POMP) phase. Using the transwell coculture system of hESCs with differentiated human corneal stromal cells, we induced hESCs to differentiate into POMPs. Then, CEC-like cells were derived from POMPs with lens epithelial cell-conditioned medium. Within 1 week, CEC-like cells that expressed the corneal endothelium (CE) differentiation marker N-cadherin and transcription factors FoxC1 and Pitx2 were detectable. Fluorescence-activated cell sorting (FACS)-based isolation of the N-cadherin/vimentin dual-positive population enriches for CEC-like cells. The isolated CEC-like cells were labeled with carboxyfluorescein diacetate, succinimidyl ester (CFDA SE) and seeded onto posterior acellular porcine corneal matrix lamellae to construct the CEC-like cell sheets. Pump function parameters of the CEC-like cell sheets approximated those of human donor corneas. Importantly, when the CEC-like cell sheets were transplanted into the eyes of rabbit CE dysfunction models, the corneal transparency was restored gradually. In conclusion, CEC-like cells derived from hESCs displayed characteristics of native human CECs. This renewable source of human CECs offers massive cells for further studies of human CEC biological characteristics and potential applications of replacement therapies as substitution for donor CECs in the future.
1 Department of Ophthalmology, Qilu Hospital, Shandong University , Jinan, People's Republic of China .
Kai
K
Zhang
Kunpeng
K
Pang
Xinyi
X
Wu
D000818:Animals; D015820:Cadherins / Q000096:biosynthesis; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D018920:Coculture Techniques / Q000379:methods; D016039:Corneal Transplantation; D017077:Culture Media, Conditioned; D053595:Embryonic Stem Cells / Q000637:transplantation*; D004728:Endothelium, Corneal / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D008648:Mesoderm / Q000166:cytology; D011817:Rabbits
D015820:Cadherins; D017077:Culture Media, Conditioned