Proteogenomic characterization of antimicrobial resistance in extensively drug-resistant Acinetobacter baumannii DU202.
OBJECTIVE
To determine the genomic sequence of extensively drug-resistant Acinetobacter baumannii DU202 and to perform proteomic characterization of antibiotic resistance in this strain using genome data.


METHODS
The genome sequence of A. baumannii DU202 was determined using the Hi-Seq 2000 system and comparative analysis was performed to determine the unique characteristics of A. baumannii DU202. Previous proteomic results from the cell wall membrane fraction by one-dimensional electrophoresis and liquid chromatography combined with mass spectrometry analysis (1DE-LC-MS/MS), using the A. baumannii ATCC 17978 genome as a reference, were reanalysed to elucidate the resistance mechanisms of A. baumannii DU202 using strain-specific genome data. Additional proteomic data from the cytosolic fraction were also analysed.


RESULTS
The genome of A. baumannii DU202 consists of 3660 genes and is most closely related to the Korean A. baumannii 1656-2 strain. More than 144 resistance genes were annotated in the A. baumannii DU202 genome, of which 72 that encoded proteins associated with antibiotic resistance were identified in the proteomic analysis of A. baumannii DU202 cultured in tetracycline, imipenem and Luria-Bertani broth (control) medium. Strong induction of β-lactamases, a multidrug resistance efflux pump and resistance-nodulation-cell division (RND) multidrug efflux proteins was found to be important in the antibiotic resistance responses of A. baumannii DU202.


CONCLUSIONS
Combining genomic and proteomic methods provided comprehensive information about the unique antibiotic resistance responses of A. baumannii DU202.
Division of Life Science, Korea Basic Science Institute, Daejeon, 305-806 Daejeon, Korea.
Sang-Yeop
SY
Lee
Division of Life Science, Korea Basic Science Institute, Daejeon, 305-806 Daejeon, Korea.
Sung Ho
SH
Yun
Division of Life Science, Korea Basic Science Institute, Daejeon, 305-806 Daejeon, Korea.
Yeol Gyun
YG
Lee
Division of Life Science, Korea Basic Science Institute, Daejeon, 305-806 Daejeon, Korea.
Chi-Won
CW
Choi
Department of Biological Science, College of Natural Science, Dong-A University, Busan 604-714, Korea.
Sun-Hee
SH
Leem
Division of Life Science, Korea Basic Science Institute, Daejeon, 305-806 Daejeon, Korea.
Edmond Changkyun
EC
Park
Division of Life Science, Korea Basic Science Institute, Daejeon, 305-806 Daejeon, Korea.
Gun-Hwa
GH
Kim
Department of Microbiology, Kyungpook National University School of Medicine, Daegu 700-422, Korea.
Je Chul
JC
Lee
Division of Life Science, Korea Basic Science Institute, Daejeon, 305-806 Daejeon, Korea Department of Bio-Analytical Science, University of Science and Technology (UST), Daejeon 305-350, Korea ksi@kbsi.re.kr.
Seung Il
SI
Kim
D040981:Acinetobacter baumannii / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004251:DNA Transposable Elements; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016680:Genome, Bacterial; D044404:Genomic Islands; D023281:Genomics; D008969:Molecular Sequence Data; D010802:Phylogeny; D040901:Proteomics; D016415:Sequence Alignment
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004251:DNA Transposable Elements
A. baumannii; LC-based proteomics; antibiotic resistance genes; comparative genomics