[Study on intestinal absorption of formononetin in Millettia nitita var. hirsutissima in rats].
To use the single-pass intestine perfusion (SPIP) model and HPLC to determine the concentration of formononetin, the effect of quality concentrations of formononetin, different intestinal segments and P-glycoprotein inhibitor on intestinal absorption of formononetin, in order to observe the intestinal absorption mechanism of formononetin from Millettia nitita var. hirsutissima in rats. The experimental results showed that the qulaity concentration of formononetin in the perfusate had no significant effect on the absorption rate constant (K(a)) and the apparent absorption coefficient (P(app)); K(a) and P(app) of formononetin in duodenum, jejunum and ileum showed no significant difference. However, K(a) was significantly higher than that in colon (P < 0.05), with significant difference between that in intestinum tenue and colon. P-glycoprotein inhibitor verapamil showed significant difference in K(a) and P(app) in intestinal segments (P < 0.05). This indicated that the absorption mechanism of formononein in rat intestinal tracts passive diffusion, without any saturated absorption. Formononein is absorbed well in all intestines. Their absorption windows were mainly concentrated in the intestinum tenue, without specific absorption sites. Formononein may be the substrate of P-glycoprotein.
National Engineering Center for Solid Preparation of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China. 582685133@qq.com
Ya-Li
YL
Liu
Department of Medical Instrument, No. 94 Hospital of PLA, Nanchang 330002, China.
Xian-Bing
XB
Xiong
National Engineering Center for Solid Preparation of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China.
Dan
D
Su
National Engineering Center for Solid Preparation of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China.
Yong-Gui
YG
Song
National Engineering Center for Solid Preparation of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China.
Ling
L
Zhang
National Engineering Center for Solid Preparation of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China.
Shi-Lin
SL
Yang
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D006801:Humans; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000737:chemistry; D007529:Isoflavones / Q000493:pharmacokinetics*; D007700:Kinetics; D008297:Male; D033641:Millettia / Q000737:chemistry*; D051381:Rats
D004365:Drugs, Chinese Herbal; D007529:Isoflavones; C007768:formononetin