Swimming behavior of Pseudomonas aeruginosa studied by holographic 3D tracking.
Holographic 3D tracking was applied to record and analyze the swimming behavior of Pseudomonas aeruginosa. The obtained trajectories allow to qualitatively and quantitatively analyze the free swimming behavior of the bacterium. This can be classified into five distinct swimming patterns. In addition to the previously reported smooth and oscillatory swimming motions, three additional patterns are distinguished. We show that Pseudomonas aeruginosa performs helical movements which were so far only described for larger microorganisms. Occurrence of the swimming patterns was determined and transitions between the patterns were analyzed.
Applied Physical Chemistry, Ruprecht-Karls-University Heidelberg, Germany ; Institute for Functional Interfaces, IFG, Karlsruhe Institute of Technology, Karlsruhe, Germany.
Svenja M
SM
Vater
Applied Physical Chemistry, Ruprecht-Karls-University Heidelberg, Germany ; Institute for Functional Interfaces, IFG, Karlsruhe Institute of Technology, Karlsruhe, Germany.
Sebastian
S
Weiße
Applied Physical Chemistry, Ruprecht-Karls-University Heidelberg, Germany ; Institute for Functional Interfaces, IFG, Karlsruhe Institute of Technology, Karlsruhe, Germany ; Analytical Chemistry-Biointerfaces, Ruhr-University Bochum, Germany.
Stojan
S
Maleschlijski
Institute for Functional Interfaces, IFG, Karlsruhe Institute of Technology, Karlsruhe, Germany.
Carmen
C
Lotz
Applied Physical Chemistry, Ruprecht-Karls-University Heidelberg, Germany.
Florian
F
Koschitzki
Institute for Functional Interfaces, IFG, Karlsruhe Institute of Technology, Karlsruhe, Germany.
Thomas
T
Schwartz
Institute for Functional Interfaces, IFG, Karlsruhe Institute of Technology, Karlsruhe, Germany.
Ursula
U
Obst
Institute for Functional Interfaces, IFG, Karlsruhe Institute of Technology, Karlsruhe, Germany ; Analytical Chemistry-Biointerfaces, Ruhr-University Bochum, Germany.
Axel
A
Rosenhahn
D011550:Pseudomonas aeruginosa / Q000166:cytology* / Q000502:physiology*