[Study on determination of caffeic acid, chlorogenic acid in rat plasma and their pharmacokinetics with LC-MS/MS].
To establish a LC-MS/MS method to determine caffeic acid, chlorogenic acid in rat plasma and study their pharmacokinetics in rats. Six Sprague-Dawley rats were intravenously injected with 4 mL x kg(-1) of Dengzhanxixin injection, respectively. Their drug plasma concentration was determined by LC-MS/MS, with tinidazole as an internal standard. The pharmacokinetic parameters were calculated by DAS 1.0. The linear concentration ranges of caffeic acid, and chlorogenic acid were 2-128 microg x L(-1) (r = 0.998 1) and 3-384 microg x L(-1) (r = 0.998 7), respectively. The methodological test showed conformance to the requirements. The intraday and inter-day variable coefficients were both less than 10.0%, indicating that both of legitimate precise and accuracy were in conformity with the requirements of biological sample analysis. For caffeic acid, the pharmacokinetic parameter t1/2beta AUC0-t, and CL were (130.91 +/- 38.77) min, (4.89 +/- 0.96) mg x min x L(-1) and (0.12 +/- 0.02) L x min(-1) x kg(-1), respectively. For chlorogenic acid, the pharmacokinetic parameter t1/2beta , AUC0-t, and CL were (49.38 +/- 8.85) min, (9.54 +/- 0.95) mg x min x L(-1) and (0.09 +/- 0.003) L x min(-1) x kg(-1), respectively. The LC-MS/MS analysis method established in this study was proved to be so accurate and sensitive that it can be applied to the pharmacokinetic study of caffeic acid and chlorogenic acid.
College of Pharmacy of Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China.
Guo-Liang
GL
Dai
College of Pharmacy of Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China.
Shi-Tang
ST
Ma
Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China.
Shi-Jia
SJ
Liu
College of Pharmacy of Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China.
Xiao-Gui
XG
Cheng
College of Pharmacy of Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China.
Yu-Xin
YX
Zang
Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China.
Wen-Zheng
WZ
Ju
Department of Clinical Pharmacology, General Hospital of Nanjing Military Area Command, Nanjing 210002, China.
Heng-Shan
HS
Tan
D000818:Animals; D002109:Caffeic Acids / Q000097:blood / Q000493:pharmacokinetics*; D002726:Chlorogenic Acid / Q000097:blood / Q000493:pharmacokinetics*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000493:pharmacokinetics*; D005260:Female; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods*
D002109:Caffeic Acids; D004365:Drugs, Chinese Herbal; D002726:Chlorogenic Acid; C040048:caffeic acid