Morphogenesis in the red alga, Griffithsia pacifica: Regeneration from single cells.
The regeneration of plants of the red alga Griffithsia pacifica from single, isolated cells is described. Regeneration can start from any cell and is triggered by the removal of an abutting cell. An isolated, single shoot cell forms a shoot and a rhizoidal cell within one day. The shoot then adds new cells by apical division at the rate of 1-2 cells/day; branches are formed at predictable but not fixed locations by budding of subapical cells. Each shoot cell enlarges for 6-8 days. The resulting plant consists of uniseriate, pseudodichotomously-branched shoot filaments with multicellular rhizoidal filaments at their base. The predictability and rapidity of this development combined with the large size of these cells (1.0×0.2 mm) facilitate developmental studies on this organism.
Department of Botany, University of Washington, Seattle.
E C
EC
Duffield
S D
SD
Waaland
R
R
Cleland