Immobilization of an integral membrane protein for biotechnological phenylacetaldehyde production.
Styrene oxide isomerase (SOI) has previously been shown to be an integral membrane protein performing a highly selective, hydrolytic ring opening reaction of epoxides to yield pure aldehydes. Earlier studies had also shown a high sensitivity of SOIs toward their product phenylacetaldehyde which caused an irreversible inhibition and finally complete loss of activity at higher aldehyde concentrations. Here we report on the covalent immobilization of a styrene oxide isomerase (SOI) on SBA-15 silica carriers. The production of the SOI from a Rhodococcus strain was optimized, the enzyme was enriched and immobilized, and finally the biocatalyst was applied in aqueous as well as in two-phase systems. Linkage of the protein to epoxide or amino groups on the SBA-based carriers led to relatively poor stabilization of the enzyme in an aqueous system. But, improved stability was observed toward organic phases like the non-toxic phthalate-related 1,2-cyclohexane dicarboxylic acid diisononyl ester (Hexamol DINCH) which here to our knowledge was used for the first time in a biotechnological application. With this two-phase system and the immobilized SOI, 1.6-2.0× higher product yields were reached and the lifetime of the biocatalyst was tremendously increased.
Interdisciplinary Ecological Center, Environmental Microbiology Group, TU Bergakademie Freiberg, Leipziger Str. 29, 09599 Freiberg, Germany. Electronic address: michel.oelschlaegel@ioez.tu-freiberg.de.
Michel
M
Oelschlägel
Interdisciplinary Ecological Center, Environmental Microbiology Group, TU Bergakademie Freiberg, Leipziger Str. 29, 09599 Freiberg, Germany.
Anika
A
Riedel
Department of Chemical Engineering, Silesian University of Technology, M. Strzody 7, 44-100 Gliwice, Poland.
Aurelia
A
Zniszczoł
Department of Chemical Engineering, Silesian University of Technology, M. Strzody 7, 44-100 Gliwice, Poland.
Katarzyna
K
Szymańska
Department of Chemical Engineering, Silesian University of Technology, M. Strzody 7, 44-100 Gliwice, Poland; Institute of Chemical Engineering, Polish Academy of Sciences, Bałtycka 5, 44-100 Gliwice, Poland.
Andrzej B
AB
Jarzębski
Interdisciplinary Ecological Center, Environmental Microbiology Group, TU Bergakademie Freiberg, Leipziger Str. 29, 09599 Freiberg, Germany.
Michael
M
Schlömann
Interdisciplinary Ecological Center, Environmental Microbiology Group, TU Bergakademie Freiberg, Leipziger Str. 29, 09599 Freiberg, Germany.
Dirk
D
Tischler
D000079:Acetaldehyde / Q000031:analogs & derivatives* / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D001709:Biotechnology; D002462:Cell Membrane / Q000378:metabolism; D003509:Cyclohexanecarboxylic Acids / Q000737:chemistry; D004795:Enzyme Stability; D007535:Isomerases / Q000378:metabolism*; D012240:Rhodococcus / Q000145:classification / Q000201:enzymology; D012822:Silicon Dioxide / Q000378:metabolism*; D012997:Solvents / Q000737:chemistry
D001426:Bacterial Proteins; D003509:Cyclohexanecarboxylic Acids; C509969:SBA-15; D012997:Solvents; D012822:Silicon Dioxide; D007535:Isomerases; C113140:styrene-oxide isomerase; D000079:Acetaldehyde; C013192:phenylacetaldehyde
1,2-Cyclohexane dicarboxylic acid diisononyl ester; Hexamol; Isomerization; Rhodococcus; Styrene oxide; Two-phase system