Profiling of glycan receptors for minute virus of mice in permissive cell lines towards understanding the mechanism of cell recognition.
The recognition of sialic acids by two strains of minute virus of mice (MVM), MVMp (prototype) and MVMi (immunosuppressive), is an essential requirement for successful infection. To understand the potential for recognition of different modifications of sialic acid by MVM, three types of capsids, virus-like particles, wild type empty (no DNA) capsids, and DNA packaged virions, were screened on a sialylated glycan microarray (SGM). Both viruses demonstrated a preference for binding to 9-O-methylated sialic acid derivatives, while MVMp showed additional binding to 9-O-acetylated and 9-O-lactoylated sialic acid derivatives, indicating recognition differences. The glycans recognized contained a type-2 Galβ1-4GlcNAc motif (Neu5Acα2-3Galβ1-4GlcNAc or 3'SIA-LN) and were biantennary complex-type N-glycans with the exception of one. To correlate the recognition of the 3'SIA-LN glycan motif as well as the biantennary structures to their natural expression in cell lines permissive for MVMp, MVMi, or both strains, the N- and O-glycans, and polar glycolipids present in three cell lines used for in vitro studies, A9 fibroblasts, EL4 T lymphocytes, and the SV40 transformed NB324K cells, were analyzed by MALDI-TOF/TOF mass spectrometry. The cells showed an abundance of the sialylated glycan motifs recognized by the viruses in the SGM and previous glycan microarrays supporting their role in cellular recognition by MVM. Significantly, the NB324K showed fucosylation at the non-reducing end of their biantennary glycans, suggesting that recognition of these cells is possibly mediated by the Lewis X motif as in 3'SIA-Le(X) identified in a previous glycan microarray screen.
Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, Florida, United States of America.
Sujata
S
Halder
Department of Laboratory Medicine, Yale School of Medicine, New Haven, Connecticut, United States of America.
Susan
S
Cotmore
Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia, United States of America.
Jamie
J
Heimburg-Molinaro
Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia, United States of America.
David F
DF
Smith
Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia, United States of America.
Richard D
RD
Cummings
Department of Chemistry, University of California Davis, Davis, California, United States of America.
Xi
X
Chen
Division of Molecular Biosciences, Imperial College London, London, United Kingdom.
Alana J
AJ
Trollope
Division of Molecular Biosciences, Imperial College London, London, United Kingdom.
Simon J
SJ
North
Division of Molecular Biosciences, Imperial College London, London, United Kingdom.
Stuart M
SM
Haslam
Division of Molecular Biosciences, Imperial College London, London, United Kingdom.
Anne
A
Dell
Department of Laboratory Medicine, Yale School of Medicine, New Haven, Connecticut, United States of America.
Peter
P
Tattersall
Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, Florida, United States of America.
Robert
R
McKenna
Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, Florida, United States of America.
Mavis
M
Agbandje-McKenna
D000818:Animals; D002213:Capsid / Q000378:metabolism; D002460:Cell Line; D005347:Fibroblasts; D006801:Humans; D008214:Lymphocytes; D051379:Mice / Q000821:virology*; D046228:Microarray Analysis; D008915:Minute Virus of Mice / Q000378:metabolism*; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014771:Virion / Q000378:metabolism
D019158:N-Acetylneuraminic Acid