Mass spectrometric identification of ancient proteins as potential molecular biomarkers for a 2000-year-old osteogenic sarcoma.
Osteosarcoma is the most common primary malignant tumor of bone usually occurring in young adolescent and children. This disease has a poor prognosis, because of the metastases in the period of tumor progression, which are usually developed previous to the clinical diagnosis. In this paper, a 2000-year-old ancient bone remain with osteogenic sarcoma was analyzed searching for tumor biomarkers which are closely related to this disease. After a specific extraction SDS-PAGE gel electrophoresis followed by tryptic digestion was performed. After the digestion the samples were measured using MALDI TOF/TOF MS. Healthy bone samples from same archaeological site were used as control samples. Our results show that in the pathological skeletal remain several well known tumor biomarkers are detected such as annexin A10, BCL-2-like protein, calgizzarin, rho GTPase-activating protein 7, HSP beta-6 protein, transferrin and vimentin compared to the control samples. The identified protein biomarkers can be useful in the discovery of malignant bone lesions such as osteosarcoma in the very early stage of the disease from paleoanthropological remains.
Department of Analytical Biochemistry, Institute of Biochemistry and Medical Chemistry, University of Pecs, Pecs, Hungary.
Agnes
A
Bona
Department of Analytical Biochemistry, Institute of Biochemistry and Medical Chemistry, University of Pecs, Pecs, Hungary.
Zoltan
Z
Papai
Department of Analytical Biochemistry, Institute of Biochemistry and Medical Chemistry, University of Pecs, Pecs, Hungary ; Janos Szentagothai Research Center, University of Pecs, Pecs, Hungary ; Imaging Center for Life and Material Sciences, University of Pecs, Pecs, Hungary ; PTE-MTA Human Reproduction Research Group, Pecs, Hungary.
Gabor
G
Maasz
Institute of Biology, University of West Hungary, Szombathely, Hungary.
Gabor A
GA
Toth
Department of Analytical Biochemistry, Institute of Biochemistry and Medical Chemistry, University of Pecs, Pecs, Hungary ; Imaging Center for Life and Material Sciences, University of Pecs, Pecs, Hungary.
Eva
E
Jambor
Department of Analytical Biochemistry, Institute of Biochemistry and Medical Chemistry, University of Pecs, Pecs, Hungary ; Janos Szentagothai Research Center, University of Pecs, Pecs, Hungary ; Imaging Center for Life and Material Sciences, University of Pecs, Pecs, Hungary.
Janos
J
Schmidt
Department of Pathology, University Teaching Hospital Markusovszky, Szombathely, Hungary.
Csaba
C
Toth
Department of Archaeology, Vas County Museums' Directorate, Szombathely, Hungary.
Csilla
C
Farkas
Department of Analytical Biochemistry, Institute of Biochemistry and Medical Chemistry, University of Pecs, Pecs, Hungary ; Janos Szentagothai Research Center, University of Pecs, Pecs, Hungary ; Imaging Center for Life and Material Sciences, University of Pecs, Pecs, Hungary ; PTE-MTA Human Reproduction Research Group, Pecs, Hungary.
Laszlo
L
Mark
D001106:Archaeology; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D001842:Bone and Bones / Q000378:metabolism* / Q000473:pathology; D004591:Electrophoresis, Polyacrylamide Gel; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D049690:History, Ancient; D006801:Humans; D006814:Hungary; D013058:Mass Spectrometry; D012516:Osteosarcoma / Q000235:genetics / Q000266:history* / Q000378:metabolism*
D014408:Biomarkers, Tumor