[Effect of huazhuo jiedu hewei recipe on the mechanism of angiogenesis in precancerous lesions of gastric cancer rats].
OBJECTIVE
To explore the possible angiogenesis mechanism of Huazhuo Jiedu Hewei Recipe (HJHR) in preventing and treating precancerous lesions of gastric cancer (PLGC).


METHODS
Totally 66 Wistar rats were randomly divided into 6 groups, i.e., the normal control group, the model group, the retinoic acid (RA) group, the high dose HJHR group, the middle dose HJHR group, the low dose HJHR group, 11 in each group. PLGC model was duplicated by inserting a spring with Helicobacter. Corresponding medicines were administered to rats in each medicated group once daily by gastrogavage, 2 mL each time for 12 successive weeks. The effect of HJHR on hypoxia induced factor (HIF-1alpha) and vascular endothelial growth factor (VEGF) of PLGC in chronic atrophic gastritis (CAG) rats' gastric mucosa was observed by immunohistochemical assay and Western blot method.


RESULTS
Compared with the normal control group, the expression of VEGF and HIF-1alpha increased in the model group (P < 0.05). Compared with the model group, the expression of VEGF and HIF-1alpha decreased in each medicated group (P < 0.05). Besides, they were lower in the high and middle dose HJHR groups than in the RA group and the low dose HJHR group (P < 0. 05). There was no statistical difference between the low dose HJHR group and the RA group (P > 0.05).


CONCLUSIONS
HJHR could prevent and treat PLGC of CAG rats possibly through decreasing the expression of HIF-1alpha and VEGF in a dose-dependent manner.
Division of Clinics, Hebei Medical University, Shijiazhuang (050020), China.
Shao-fang
SF
Gao
Department of Digestive Diseases, Hebei Provincial Hospital of Chinese Medicine, Shijiazhuang (050011), China.
Yan-gang
YG
Wang
Department of Digestive Diseases, Hebei Provincial Hospital of Chinese Medicine, Shijiazhuang (050011), China.
Dian-gui
DG
Li
Department of Digestive Diseases, Hebei Provincial Hospital of Chinese Medicine, Shijiazhuang (050011), China.
Lin
L
Pei
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005753:Gastric Mucosa / Q000378:metabolism; D005756:Gastritis / Q000378:metabolism / Q000382:microbiology; D016998:Helicobacter; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D008297:Male; D009389:Neovascularization, Pathologic; D011230:Precancerous Conditions / Q000098:blood supply* / Q000188:drug therapy / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013274:Stomach Neoplasms / Q000098:blood supply* / Q000188:drug therapy / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
D004365:Drugs, Chinese Herbal; C497444:Hif1a protein, rat; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat