Scale-up cultivation of Chlorella ellipsoidea from indoor to outdoor in bubble column bioreactors.
The cultivation of Chlorella ellipsoidea in bubble column bioreactors was investigated at different scales under indoor and outdoor conditions. The algal cells were able to quickly adapt to the outdoor conditions and achieved a growth rate of 31.55mg L(-1)day(-1). Due to differences in light and temperature, the outdoor culture produced a higher percentage of unsaturated fatty acids compared to the indoor cultures, while the amino acid composition was unaffected. The overall cost of the biomass produced by the 200L outdoor cultivation (58.70US$/kg-dry weight) was estimated to be more than 7 times lower than that of the 20L indoor cultivation (431.39US$/kg-dry weight). Together these results provide a basis for the cultivation of C. ellipsoidea for the large-scale production of biofuels, high-value nutrients and/or recombinant proteins.
National Key Laboratory of Biochemical Engineering & Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
Shi-Kai
SK
Wang
National Key Laboratory of Biochemical Engineering & Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
Yi-Ru
YR
Hu
National Key Laboratory of Biochemical Engineering & Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
Feng
F
Wang
Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA.
Amanda R
AR
Stiles
National Key Laboratory of Biochemical Engineering & Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China. Electronic address: czliu@home.ipe.ac.cn.
Chun-Zhao
CZ
Liu
D020418:Algal Proteins / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000191:economics / Q000295:instrumentation* / Q000379:methods*; D002708:Chlorella / Q000254:growth & development*; D003365:Costs and Cost Analysis; D005227:Fatty Acids / Q000378:metabolism; D007700:Kinetics
D020418:Algal Proteins; D000596:Amino Acids; D005227:Fatty Acids
Bubble column bioreactor; Chlorella ellipsoidea; Economic evaluation; Fatty acid composition; Outdoor cultivation