Tailoring chimeric ligands for studying and biasing ErbB receptor family interactions.
Described is the development and application of a versatile semisynthetic strategy, based on a combination of sortase-mediated coupling and tetrazine ligation chemistry, which can be exploited for the efficient incorporation of tunable functionality into chimeric recombinant proteins. To demonstrate the scope of the method, the assembly of a set of bivalent ligands, which integrate members of the epidermal growth factor (EGF) ligand family, is described. By using a series of bivalent EGFs with variable intraligand spacing, the differences in structure were correlated with the ability to bias signaling in the ErbB receptor family in a cell motility assay. Biasing away from EGFR-HER2 dimerization with a bivalent EGF was observed to reduce cell motility in an intraligand distance-dependent fashion, thus demonstrating the utility of the approach for acutely perturbing receptor-mediated cell signaling pathways.
Departments of Biology and Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge, MA (USA).
Andrew T
AT
Krueger
Carsten
C
Kroll
Edgar
E
Sanchez
Linda G
LG
Griffith
Barbara
B
Imperiali
D066246:ErbB Receptors / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008024:Ligands; D059630:Mesenchymal Stem Cells / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000737:chemistry* / Q000378:metabolism
D066246:ErbB Receptors; D008024:Ligands; D018719:Erb-b2 Receptor Tyrosine Kinases
biological activity; ligand design; protein modifications; receptors; structure-activity relationships