[Effect of orthodontic force on expression of Asporin after tooth auto-transplantation: an experimental study in Beagle dogs].
OBJECTIVE
To evaluate the effect of orthodontic light force on the expression of Asporin, bone morphogenetic proteins-2 (BMP-2) and alkaline phosphatase (ALP) after auto-transplantation.


METHODS
Thirty-two maxillary and mandibular incisors in four 13 month-old male Beagle dogs were auto-transplanted to the other side of the same jaw. The teeth were all endodontically treated and divided into four groups, control (group 1) and three experimental groups (groups 2, 3 and 4).In control group, the teeth were unloaded. In the other three experimental groups, continuous force was applied in the 1st week (group 2), 2nd week (group 3) and 4th week (group 4) after auto-transplantation, respectively. The dogs were sacrificed in the 8th week. The mRNA expressions of Asporin,BMP-2 and ALP were examined by real time PCR. The expression of periodontal ligament associated protein-1 (PLAP-1) was examined by Western blotting. The data were statistically analyzed using SPSS 17.0 and one-way analysis of variance (ANOVA).


RESULTS
In group 3, the expression of Asporin mRNA (2.047 ± 0.281) was higher than that in the other three groups, while the expression of BMP-2 (1.358 ± 0.095) was lower than that in group 2 and control group (P < 0.05). The expression of PLAP-1 (1.054 ± 0.113) in group 3 was higher than other groups, while significant difference was found between any two groups.


CONCLUSIONS
Orthodontic force could stimulate the expression of Asporin and PLAP-1. The orthodontic force applied in the 2nd week after the tooth auto-transplantation, the expression of Asporin and PLAP-1 reached the highest level.
Department of Orthodontics, Capital Medical University School of Stomatology, Beijing 100050, China.
Shi-chao
SC
Yue
Department of Orthodontics, Capital Medical University School of Stomatology, Beijing 100050, China. Email: byuxing@263.net.
Yu-xing
YX
Bai
Department of Orthodontics, Capital Medical University School of Stomatology, Beijing 100050, China.
Yun
Y
Yang
Department of Orthodontics, Capital Medical University School of Stomatology, Beijing 100050, China.
Li-xuan
LX
Li
D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics / Q000378:metabolism*; D004285:Dogs; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D007180:Incisor / Q000378:metabolism / Q000637:transplantation*; D008297:Male; D008334:Mandible; D008437:Maxilla; D050793:Orthodontic Extrusion; D012333:RNA, Messenger / Q000378:metabolism; D014182:Transplantation, Autologous
D055396:Bone Morphogenetic Protein 2; D016326:Extracellular Matrix Proteins; D012333:RNA, Messenger; D000469:Alkaline Phosphatase