Ghrelin administered spinally increases the blood glucose level in mice.
Ghrelin is known as a regulator of the blood glucose homeostasis and food intake. In the present study, the possible roles of ghrelin located in the spinal cord in the regulation of the blood glucose level were investigated in ICR mice. We found that intrathecal (i.t.) injection with ghrelin (from 1 to 10 μg) caused an elevation of the blood glucose level. In addition, i.t. pretreatment with YIL781 (ghrelin receptor antagonist; from 0.1 to 5 μg) markedly attenuated ghrelin-induced hyperglycemic effect. The plasma insulin level was increased by ghrelin. The enhanced plasma insulin level by ghrelin was reduced by i.t. pretreatment with YIL781. However, i.t. pretreatment with glucagon-like peptide-1 (GLP-1; 5 μg) did not affect the ghrelin-induced hyperglycemia. Furthermore, i.t. administration with ghrelin also elevated the blood glucose level, but in an additive manner, in d-glucose-fed model. Our results suggest that the activation of ghrelin receptors located in the spinal cord plays important roles for the elevation of the blood glucose level.
Department of Pharmacology, Institute of Natural Medicine, College of Medicine, Hallym University, 39 Hallymdaehak-gil, Chuncheon, Gangwon-do 200-702, Republic of Korea.
Yun-Beom
YB
Sim
Department of Pharmacology, Institute of Natural Medicine, College of Medicine, Hallym University, 39 Hallymdaehak-gil, Chuncheon, Gangwon-do 200-702, Republic of Korea.
Soo-Hyun
SH
Park
Department of Pharmacology, Institute of Natural Medicine, College of Medicine, Hallym University, 39 Hallymdaehak-gil, Chuncheon, Gangwon-do 200-702, Republic of Korea.
Sung-Su
SS
Kim
Department of Pharmacology, Institute of Natural Medicine, College of Medicine, Hallym University, 39 Hallymdaehak-gil, Chuncheon, Gangwon-do 200-702, Republic of Korea.
Chea-Ha
CH
Kim
Department of Pharmacology, Institute of Natural Medicine, College of Medicine, Hallym University, 39 Hallymdaehak-gil, Chuncheon, Gangwon-do 200-702, Republic of Korea.
Su-Jin
SJ
Kim
Department of Pharmacology, Institute of Natural Medicine, College of Medicine, Hallym University, 39 Hallymdaehak-gil, Chuncheon, Gangwon-do 200-702, Republic of Korea.
Su-Min
SM
Lim
Department of Pharmacology, Institute of Natural Medicine, College of Medicine, Hallym University, 39 Hallymdaehak-gil, Chuncheon, Gangwon-do 200-702, Republic of Korea.
Jun-Sub
JS
Jung
Department of Pharmacology, Institute of Natural Medicine, College of Medicine, Hallym University, 39 Hallymdaehak-gil, Chuncheon, Gangwon-do 200-702, Republic of Korea. Electronic address: hwsuh@hallym.ac.kr.
Hong-Won
HW
Suh
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D054439:Ghrelin / Q000008:administration & dosage* / Q000494:pharmacology; D052216:Glucagon-Like Peptide 1 / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D006943:Hyperglycemia / Q000139:chemically induced; D007278:Injections, Spinal; D007328:Insulin / Q000097:blood; D008297:Male; D008813:Mice, Inbred ICR; D010880:Piperidines / Q000494:pharmacology; D052999:Quinazolinones / Q000494:pharmacology; D054440:Receptors, Ghrelin / Q000037:antagonists & inhibitors; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism
D001786:Blood Glucose; D054439:Ghrelin; D007328:Insulin; D010880:Piperidines; D052999:Quinazolinones; D054440:Receptors, Ghrelin; C524424:YIL 781; D052216:Glucagon-Like Peptide 1; D005947:Glucose
Blood glucose; GLP-1; Ghrelin; Insulin; Intrathecal; Spinal cord