Development of on-line spectroscopic determination approach of dispersive liquid-liquid microextraction based on an effective device.
A novel, rapid, simple, and low-cost on-line determination approach of dispersive liquid-liquid microextraction (DLLME) with low-density solvents was developed with the support of a specially designed effective homemade device. The proposed method surmounted the drawbacks of conventional DLLME of the need of high-density solvents as extractants, and the requirement of centrifugation operation to obtain phase separation, and the difficulties to realize on-line determination. The amount of sample utilized can conveniently change according to practical needs by varying the volume of the extraction tube of the device to perform a more effective DLLME. A case study was carried out to assess this method utilizing the dye rhodamine B as the model analyte. The experiment parameters influencing the extraction were systematically investigated. Under optimum conditions, the linearity was obtained in the range of 0.015-1.000 μg/mL with the correlation coefficient (r(2)) of 0.9980. The limit of detection and quantification were 6.1 and 20.4 μg/L, respectively. Good repeatability was achieved with the relative standard deviations (RSD) for five replicate measurements of different concentration samples less than 4.06%, and the presented method was successfully employed to quantify rhodamine B in three real samples.
Shanghai Key Laboratory of Functional Materials Chemistry, and Research Centre of Analysis and Test, East China University of Science and Technology, Shanghai 200237, PR China.
Wei
W
Li
Shanghai Key Laboratory of Functional Materials Chemistry, and Research Centre of Analysis and Test, East China University of Science and Technology, Shanghai 200237, PR China.
Xuan
X
Zhang
Shanghai Key Laboratory of Functional Materials Chemistry, and Research Centre of Analysis and Test, East China University of Science and Technology, Shanghai 200237, PR China.
Peijin
P
Tong
Shanghai Key Laboratory of Functional Materials Chemistry, and Research Centre of Analysis and Test, East China University of Science and Technology, Shanghai 200237, PR China.
Ting
T
Wu
Shanghai Key Laboratory of Functional Materials Chemistry, and Research Centre of Analysis and Test, East China University of Science and Technology, Shanghai 200237, PR China.
Huilian
H
Hu
Shanghai Key Laboratory of Functional Materials Chemistry, and Research Centre of Analysis and Test, East China University of Science and Technology, Shanghai 200237, PR China.
Meng
M
Wang
Shanghai Key Laboratory of Functional Materials Chemistry, and Research Centre of Analysis and Test, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: yipingdu@ecust.edu.cn.
Yiping
Y
Du
D020003:1-Octanol / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D059627:Liquid Phase Microextraction / Q000295:instrumentation* / Q000379:methods*; D009862:Online Systems; D012235:Rhodamines / Q000302:isolation & purification; D012965:Sodium Chloride / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013053:Spectrophotometry / Q000295:instrumentation* / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
D012235:Rhodamines; D012997:Solvents; D012965:Sodium Chloride; C029773:rhodamine B; D020003:1-Octanol
Dispersive liquid–liquid microextraction; Low-density solvent; On-line determination; Quantitative analysis; Rhodamine B