High-resolution mass spectrometry applied to the study of metabolome modifications in various chicken tissues after amoxicillin administration.
The performance of high resolution accurate mass spectrometry (HRMS) operating in full scan MS mode was investigated for the quantitative determination of amoxicillin (AMX) as well as qualitative analysis of metabolomic profiles in tissues of medicated chickens. The metabolomic approach was exploited to compile analytical information on changes in the metabolome of muscle, kidney and liver from chickens subjected to a pharmacological program with AMX. Data consisting of m/z features taken throughout the entire chromatogram were extracted and filtered to be treated by Principal Component Analysis. As a result, it was found that medicated and non-treated animals were clearly clustered in distinct groups. Besides, the multivariate analysis revealed some relevant mass features contributing to this separation. In this context, recognizing those potential markers of each chicken class was a priority research for both metabolite identification and, obviously, evaluation of food quality and health effects associated to food consumption.
Department of Analytical Chemistry, Food and Nutrition Torribera Campus, University of Barcelona, Avda. Prat de la Riba, 171, Sta. Coloma de Gramenet, E-08921 Barcelona, Spain.
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Hermo
Department of Analytical Chemistry, University of Barcelona, Avda. Diagonal 645, E-08028 Barcelona, Spain.
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Saurina
Department of Analytical Chemistry, Food and Nutrition Torribera Campus, University of Barcelona, Avda. Prat de la Riba, 171, Sta. Coloma de Gramenet, E-08921 Barcelona, Spain; Department of Analytical Chemistry, University of Barcelona, Avda. Diagonal 645, E-08028 Barcelona, Spain.
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Barbosa
Department of Analytical Chemistry, Food and Nutrition Torribera Campus, University of Barcelona, Avda. Prat de la Riba, 171, Sta. Coloma de Gramenet, E-08921 Barcelona, Spain. Electronic address: dolores.barron@ub.edu.
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Barrón
D000658:Amoxicillin / Q000008:administration & dosage / Q000032:analysis* / Q000378:metabolism*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000032:analysis* / Q000378:metabolism*; D002645:Chickens / Q000378:metabolism*; D007668:Kidney / Q000737:chemistry / Q000378:metabolism; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods*; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism
D000900:Anti-Bacterial Agents; D000658:Amoxicillin
Amoxicillin; Food biomarkers; Linear Trap quadrupole-Orbitrap mass spectrometry; Metabolomics; Multivariate analysis