Using high-performance liquid chromatography with UV detector to quantify exhaled leukotriene B4 level in nonatopic adults.
This study aimed to evaluate the feasibility of the chemical method to analyze exhaled breath condensate (EBC) leukotriene B4 (LTB4) level in humans. High-performance liquid chromatography with a UV detector was applied to quantify the inflammatory biomarker. The LTB4 concentration in the concentrated pooled EBC samples was 1.19 ng/μL, and the average LTB4 concentration of each EBC sample was 15.38 ng/μL. This analytical technique was feasible to evaluate the levels of inflammatory mediators such as LTB4 in human EBCs without any complicated sample pretreatment processes.
Department of Respiratory Therapy, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
Gwo-Hwa
GH
Wan
Division of Taipei Pediatrics, Department of Pediatrics, Chang Gung Children's Hospital, Chang Gung Memorial Hospital, Taoyuan, Taiwan; Department of Respiratory Therapy, Chang Gung Memorial Hospital, Taoyuan, Taiwan; College of Medicine, Chang Gung University, Taoyuan, Taiwan.
Dah-Chin
DC
Yan
Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
Hui-Yun
HY
Tseng
School of Nursing, Chang Gung University, Taoyuan, Taiwan.
Jian-Tao
JT
Lee
Department of Public Health, College of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan. Electronic address: 056416@mail.fju.edu.tw.
Yu-Wen
YW
Lin
D000328:Adult; D015415:Biomarkers / Q000032:analysis; D001944:Breath Tests; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D045853:Exhalation; D005260:Female; D006801:Humans; D007975:Leukotriene B4 / Q000032:analysis*; D008297:Male; D055815:Young Adult
D015415:Biomarkers; D007975:Leukotriene B4
exhaled breath condensate; high-performance liquid chromatography; leukotriene B(4); leukotrienes