[Detection of the exogenous gene copy number of the transgenic tomato anti-caries vaccine].
OBJECTIVE
To detect the exogenous gene copy number of the transgenic tomato anti-caries vaccine by using the SYBR Green real-time PCR.


METHODS
Recombinant plasmid pEAC10 and pEPC10 were used as standard to detect genome samples of exogenous gene pacA-ctxB and pacP-ctxB by SYBR green fluorescent quantitation, then the average value was calculated as gene copy number.


RESULTS
The copy number of the transgenic tomato carrying pacA-ctxB was 1.3 and the pacP-ctxB was 3.2.


CONCLUSIONS
The transgenic tomato plants which have high stability are low-copy transgenic plants. Supported by National Natural Science Foundation of China (30160086, 81260164), Science and Technical Fund of Guizhou Province (LKZ[2011]41), Project of Technology Innovation Team in Guizhou Province, Leading Academic Discipline Construction Project in Guizhou Province and Excellent Scientific Research Team Cultivation Project in Zunyi Medical College ([2012]12).
The Special Key Laboratory of Oral Diseases Research, Higher Education Institution of Guizhou Province,China.E-mail:baiguohui1228@126.com.
Guo-hui
GH
Bai
Jian-guo
JG
Liu
Yuan
Y
Tian
Zhu
Z
Chen
Peng-yuan
PY
Bai
Qi
Q
Han
Yu
Y
Gu
Xiao-yan
XY
Guan
Hai-hui
HH
Wang
D003731:Dental Caries / Q000517:prevention & control*; D018628:Gene Dosage; D018551:Solanum lycopersicum; D030821:Plants, Genetically Modified; D014612:Vaccines
D014612:Vaccines