Combined pulldown and time-lapse microscopy studies for determining the role of Rap1 in the crosstalk between integrins and cadherins.
The coordinate modulation of the cellular functions of cadherins and integrins plays an essential role in fundamental physiological and pathological processes, including morphogenesis, tissue differentiation and renewal, wound healing, immune surveillance, inflammatory response, tumor progression, and metastasis. Recent findings state the molecular mechanisms underlying the fine-balanced relationship between cadherins and integrins. In particular, some of the novel results recently obtained raise the possibility of a pivotal role for the small GTPase Rap1 in the functional crosstalk between cadherins and integrins. Considering the importance of the molecular signalling triggered by Rap1, here we provide protocols to study this small GTPase in signalling pathways involving cadherins and integrins.
Department of Clinical and Biological Sciences, University of Torino, Torino, Italy.
Luca
L
Goitre
Saverio Francesco
SF
Retta
D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002448:Cell Adhesion; D002452:Cell Count; D002460:Cell Line; D004705:Endocytosis; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005109:Extracellular Matrix / Q000378:metabolism; D022001:Focal Adhesions / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D016023:Integrins / Q000378:metabolism*; D007365:Intercellular Junctions; D008853:Microscopy / Q000379:methods*; D017027:Protein Tyrosine Phosphatases / Q000037:antagonists & inhibitors; D013997:Time Factors; D020827:rap1 GTP-Binding Proteins / Q000378:metabolism*
D015820:Cadherins; D004791:Enzyme Inhibitors; D016023:Integrins; D017027:Protein Tyrosine Phosphatases; D020827:rap1 GTP-Binding Proteins; D005978:Glutathione; D002118:Calcium