Effect of Momordica dioica fruit extract on antioxidant status in liver, kidney, pancreas, and serum of diabetic rats.
BACKGROUND
Fruits, leaves, and tuberous roots of Momordica dioica are used as a folk remedy for diabetes mellitus (DM) in India. The aqueous extract of Momordica dioica fruit possesses very good anti-diabetic activity and is having high margin of safety.


OBJECTIVE
The aim of the present study was to investigate the antioxidative effect of Momordica dioica fruits in alloxan-induced diabetic Wistar rats.


METHODS
Effect of aqueous extract of Momordica dioica (AEMD) on thiobarbituric acid reactive substances (TBARS), hydroperoxide (HP), non-enzymatic and enzymatic antioxidants in liver, kidney, pancreas, and serum was evaluated in diabetic rats after 21 days treatment.


RESULTS
Increase in the levels of TBARS, HP and decrease in the levels of non-enzymatic antioxidants and activity of enzymatic antioxidants was observed in liver, kidney, pancreas, and serum of diabetic rats when compared with normal healthy rats. TBARS and HP levels were reduced while non-enzymatic and enzymatic antioxidant enzymes activity was increased in AEMD and glibenclamide-treated rats. Furthermore, histological examination of liver, kidney, and pancreas of diabetic rats showed degenerative changes. AEMD treatment for 21 days rejuvenated liver, kidney, and pancreas histoarchitecture.


CONCLUSIONS
In conclusion, the present results showed the protective role of AEMD on liver, kidney, and pancreas in severe diabetic rats justifying support for its anti-diabetic use in folk medicine.
Department of Zoology, Bundelkhand University, Jhansi, Uttar Pradesh, India.
Poonam
P
Sharma
Department of Biomedical Sciences, Bundelkhand University, Jhansi, Uttar Pradesh, India.
Rambir
R
Singh
Alloxan; Wistar rat; momordica dioica; oxidative stress