Photorhabdus heterorhabditis sp. nov., a symbiont of the entomopathogenic nematode Heterorhabditis zealandica.
The bacterial symbionts SF41T and SF783 were isolated from populations of the insect pathogenic nematode Heterorhabditis zealandica collected in South Africa. Both strains were closely related to strain Q614 isolated from a population of Heterorhabditis sp. collected from soil in Australia in the 1980s. Sequence analysis based on a multigene approach, DNA-DNA hybridization data and phenotypic traits showed that strains SF41T, SF783 and Q614 belong to the same species of the genus Photorhabdus with Photorhabdus temperata subsp. cinerea as the most closely related taxon (DNA-DNA hybridization value of 68%). Moreover, the phylogenetic position of Photorhabdus temperata subsp. cinerea DSM 19724T initially determined using the gyrB sequences, was reconsidered in the light of the data obtained by our multigene approach and DNA-DNA hybridization experiments. Strains SF41T, SF783 and Q614 represent a novel species of the genus Photorhabdus, for which the name Photorhabdus heterorhabditis sp. nov. is proposed (type strain SF41T=ATCC BAA-2479T=DSM 25263T).
Department of Conservation Ecology and Entomology, Stellenbosch University, Private Bag X1, 7602 Matieland, Stellenbosch, South Africa.
Tiarin
T
Ferreira
Department of Microbiology, Stellenbosch University, Private Bag X1, 7602 Matieland, Stellenbosch, South Africa.
Carol A
CA
van Reenen
Department of Microbiology, Stellenbosch University, Private Bag X1, 7602 Matieland, Stellenbosch, South Africa.
Akihito
A
Endo
INRA, UMR1333 Diversité, Génomes & Interactions Microorganismes-Insectes (DGIMI), F-34000 Montpellier, France.|Université Montpellier 2, UMR1333 Diversité, Génomes & Interactions Microorganismes-Insectes (DGIMI), F-34000 Montpellier, France.
Patrick
P
Tailliez
INRA, UMR1333 Diversité, Génomes & Interactions Microorganismes-Insectes (DGIMI), F-34000 Montpellier, France.|Université Montpellier 2, UMR1333 Diversité, Génomes & Interactions Microorganismes-Insectes (DGIMI), F-34000 Montpellier, France.
Sylvie
S
Pagès
DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen, Inhoffenstrasse 7B, 38124 Braunschweig, Germany.
Cathrin
C
Spröer
Department of Conservation Ecology and Entomology, Stellenbosch University, Private Bag X1, 7602 Matieland, Stellenbosch, South Africa.
Antoinette P
AP
Malan
Department of Microbiology, Stellenbosch University, Private Bag X1, 7602 Matieland, Stellenbosch, South Africa.
Leon M T
LMT
Dicks
D000818:Animals; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D007313:Insecta / Q000382:microbiology / Q000469:parasitology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D020637:Photorhabdus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017172:Rhabditoidea / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013019:South Africa; D013559:Symbiosis
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S