Chimerism status is correlated to acute graft-versus-host disease after allogeneic stem cell transplantation.
To evaluate the correlation between chimerism status and acute graft-versus-host disease (aGVHD) following allogeneic hematopoietic stem cell transplantation. Chimerism of peripheral blood of 124 patients was monitored at regular intervals post-transplant. The chimerism of 124 post-transplant cases of CD3(+)T lymphocytes, 107 cases of CD3(-)CD56(+)CD16(+)NK lymphocytes, 49 cases of CD15(+) granulocytes, and 27 cases of CD19(+)B lymphocytes sorted by fluorescence-activated cell sorter were analyzed by polymerase chain reaction amplification of short tandem repeats. Differences were found in the time between establishment of full donor T-cell chimerism and the occurrence of aGVHD (P = 0.035, two related samples test). Patients with ≥69 % donor chimerism on day +7 in T-cells had higher rates of aGVHD. This study may provide a rational basis for treatment with adoptive immunotherapy at an earlier time, such as day 7 after SCT, than at present to prevent aGVHD.
Department of Hematology, Shanghai First People's Hospital, Medical College, Shanghai Jiaotong University, 100 Haining Road, Shanghai, 200080, People's Republic of China.
Ying
Y
Jiang
Li-ping
LP
Wan
You-wen
YW
Qin
Xiao-rui
XR
Wang
Shi-ke
SK
Yan
Kuang-cheng
KC
Xie
Chun
C
Wang
D000208:Acute Disease; D064591:Allografts; D015703:Antigens, CD; D001402:B-Lymphocytes; D005434:Flow Cytometry; D006086:Graft vs Host Disease / Q000235:genetics* / Q000517:prevention & control; D018380:Hematopoietic Stem Cell Transplantation; D016219:Immunotherapy, Adoptive; D016131:Lymphocyte Subsets; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D013601:T-Lymphocytes; D013997:Time Factors; D018183:Transplantation Chimera
D015703:Antigens, CD