Expression of TRPV1 in the C57BL/6 mice brain hippocampus and cortex during development.
Transient receptor potential vanilloid 1 (TRPV1) channel has been found to be expressed in a variety of tissues over the last few years, including the central nervous system (CNS). However, the distribution of TRPV1 in the CNS remains a controversial question. Here, we reveal that the expression of TRPV1 can be detected in the C57BL/6 mouse hippocampus and cortex using real-time PCR and western blot. Beyond that, mRNA and protein expression levels of TRPV1 show dynamic changes during brain development. Compared with the earliest timepoint examined at 2 weeks, the expression levels of mRNA progressively increased at 4 and 8 weeks, peaking at the later timepoint, then declined at 16 weeks but remained elevated. However, compared with 2-week-old mice, the expression levels of the other three groups (4-, 8-, and 16-week-old mice) increased overall. These results indicate that TRPV1 channel expression is detectable in the CNS and it varies during postnatal development.
School of Basic Medical Sciences, Wuhan University, Hubei, China.
Wen-Xian
WX
Huang
Jia-Wei
JW
Min
Yu-Qiang
YQ
Liu
Xiao-Hua
XH
He
Bi-Wen
BW
Peng
D000367:Age Factors; D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000096:biosynthesis*; D050916:TRPV Cation Channels / Q000096:biosynthesis*
D012333:RNA, Messenger; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse