The cleverSuite approach for protein characterization: predictions of structural properties, solubility, chaperone requirements and RNA-binding abilities.
BACKGROUND
The recent shift towards high-throughput screening is posing new challenges for the interpretation of experimental results. Here we propose the cleverSuite approach for large-scale characterization of protein groups.


METHODS
The central part of the cleverSuite is the cleverMachine (CM), an algorithm that performs statistics on protein sequences by comparing their physico-chemical propensities. The second element is called cleverClassifier and builds on top of the models generated by the CM to allow classification of new datasets.


RESULTS
We applied the cleverSuite to predict secondary structure properties, solubility, chaperone requirements and RNA-binding abilities. Using cross-validation and independent datasets, the cleverSuite reproduces experimental findings with great accuracy and provides models that can be used for future investigations.


BACKGROUND
The intuitive interface for dataset exploration, analysis and prediction is available at http://s.tartaglialab.com/clever_suite.
Gene Function and Evolution, Centre for Genomic Regulation (CRG), Dr. Aiguader 88 and Universitat Pompeu Fabra (UPF), 08003 Barcelona, Spain.
Petr
P
Klus
Gene Function and Evolution, Centre for Genomic Regulation (CRG), Dr. Aiguader 88 and Universitat Pompeu Fabra (UPF), 08003 Barcelona, Spain.
Benedetta
B
Bolognesi
Gene Function and Evolution, Centre for Genomic Regulation (CRG), Dr. Aiguader 88 and Universitat Pompeu Fabra (UPF), 08003 Barcelona, Spain.
Federico
F
Agostini
Gene Function and Evolution, Centre for Genomic Regulation (CRG), Dr. Aiguader 88 and Universitat Pompeu Fabra (UPF), 08003 Barcelona, Spain.
Domenica
D
Marchese
Gene Function and Evolution, Centre for Genomic Regulation (CRG), Dr. Aiguader 88 and Universitat Pompeu Fabra (UPF), 08003 Barcelona, Spain.
Andreas
A
Zanzoni
Gene Function and Evolution, Centre for Genomic Regulation (CRG), Dr. Aiguader 88 and Universitat Pompeu Fabra (UPF), 08003 Barcelona, Spain.
Gian Gaetano
GG
Tartaglia
D000465:Algorithms; D064267:Intrinsically Disordered Proteins / Q000737:chemistry; D018832:Molecular Chaperones / Q000737:chemistry* / Q000378:metabolism; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry*; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D020539:Sequence Analysis, Protein; D012984:Software; D012995:Solubility
D064267:Intrinsically Disordered Proteins; D018832:Molecular Chaperones; D011506:Proteins; D016601:RNA-Binding Proteins