Malathion-induced changes in the haematological profile, the immune response, and the oxidative/antioxidant status of Cyprinus carpio carpio: protective role of propolis.
The present study investigated the potential ameliorative effects of propolis against malathion toxicity in the blood and various tissues of carp. The fish were exposed to sublethal concentrations of malathion (0.5 and 1 mg/L) for 10 days, and propolis (10 mg/kg of fish weight) was simultaneously administered. Blood and tissue (liver, kidney, and gill) samples were collected at the end of the experiment and analysed to determine the haematological profile (red blood cell count, haemoglobin concentration, haematocrit level, and erythrocyte indices: mean corpuscular volume, mean corpuscular haemoglobin, and mean corpuscular haemoglobin concentration), immune response (white blood cell count, oxidative radical production, nitroblue tetrazolium (NBT) activity, total plasma protein and total immunoglobulin levels, and the phagocytic activity), and oxidant/antioxidant status (malondialdehyde and reduced glutathione levels and superoxide dismutase, catalase, and glutathione peroxidase activities) of the fish. The findings of this study demonstrate that malathion has a negative effect on the haematological parameters, immune response, and antioxidant enzyme activities of the fish. However, the administration of propolis ameliorated the malathion-induced toxic effects.
Firat University, Fisheries Faculty, Department of Aquaculture and Fish Diseases, 23119 Elazig, Turkey. Electronic address: serpilmise@gmail.com.
Serpil Mişe
SM
Yonar
Firat University, College of Keban, Fisheries Programme, 23700 Elazig, Turkey.
Mevlüt Şener
MŞ
Ural
University of Erciyes, Seyrani Agricultural Faculty, Department of Agricultural Biotechnology, Kayseri, Turkey.
Sibel
S
Silici
Firat University, Fisheries Faculty, Department of Aquaculture and Fish Diseases, 23119 Elazig, Turkey.
M Enis
ME
Yonar
D000818:Animals; D002347:Carps / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D002374:Catalase / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D005880:Gills / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D007107:Immune System / Q000187:drug effects*; D007668:Kidney / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D008294:Malathion / Q000633:toxicity*; D008315:Malondialdehyde / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D011429:Propolis / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
D008315:Malondialdehyde; D011429:Propolis; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; D008294:Malathion
Carp; Haematology; Immunity; Malathion; Oxidative stress; Propolis