Simultaneous determination of erlotinib and metabolites in human urine using capillary electrophoresis.
The purpose of this study was to develop a simple and sensitive CE-UV method to quantify erlotinib and metabolites in urine. Following liquid-liquid extraction, erlotinib, and metabolites were separated with a BGE whose composition was phosphate buffer (pH 2.5, 65 mM) with 0.5% Tween 20. The applied voltage was 22 kV, capillary temperature 25°C and the sample injection was performed in the hydrodynamic mode. All the analyses were carried out in a fused silica capillary with an internal diameter of 75 μm and a total length of 37 cm. The detection of target compounds was performed at 240 nm. The calibration was linear in the range 0.15-20 mg/L for erlotinib and metabolites. Inter-and intraday imprecision were less than 4%. This simple, sensitive, accurate, and cost-effective method can be used in routine clinical practice to monitor erlotinib concentrations in urine from nonsmall cell lung cancer patients.
Department of Analytical Chemistry and Food Technology, University of Castilla-La Mancha, Ciudad Real, Spain.
Juana
J
Rodríguez
Gregorio
G
Castañeda
Lorena
L
Muñoz
Diana
D
Navarro
Jose C
JC
Villa
D002138:Calibration; D019075:Electrophoresis, Capillary / Q000379:methods*; D000069347:Erlotinib Hydrochloride; D006801:Humans; D057230:Limit of Detection; D011799:Quinazolines / Q000652:urine*; D015203:Reproducibility of Results
D011799:Quinazolines; D000069347:Erlotinib Hydrochloride
CE; Erlotinib; Lung cancer; Urine