(99m) T c-DTPA Study to Validate an Experimental Model of Ureteral Obstruction in Rabbits: Preliminary Results.
Objective. To create a ureteral obstruction experimental model that can be proved through (99m)Tc-DTPA renal scintigraphy and histopathological studies, without causing total renal function loss. Materials and Methods. Ten New Zealand white rabbits were submitted to a surgical experiment to create a model of unilateral obstruction to urinary flow. Surgery procedure provided unilateral ureteral obstruction (left kidney) to urinary flow and posteriorly was evaluated by (99m)Tc-DTPA renal scintigraphy and histopathological study. (99m)Tc-DTPA renal study was performed to detect and quantify signs of obstruction and to evaluate renal function. Statistical analysis was performed through the Student t-test with a significance level of P<0.05. Results. Nine of the ten rabbits presented left renal unit obstruction and one nonobstructive on the (99m)Tc-DTPA and histopathological studies. All the right renal units, which were not submitted to surgical procedure, were nonobstructed by the studies. There was a general agreement between scintigraphy and histopathological results in both groups. Conclusion. The experimental model promoted the creation of ureteral obstruction in rabbits, confirmed by nuclear medicine scintigraphy and histopathology, and could be used in further studies to better understand urinary obstruction.
Division of Urology, Department of Surgery, Campinas State University, Avenida Vital Brasil 251, Caixa Postal 6142, Cidade Universitária Zeferino Vaz, 13083-888 Campinas, SP, Brazil.
Marcelo Lopes
ML
de Lima
Division of Urology, Department of Surgery, Campinas State University, Avenida Vital Brasil 251, Caixa Postal 6142, Cidade Universitária Zeferino Vaz, 13083-888 Campinas, SP, Brazil.
Rodolfo
R
Bertti
Division of Urology, Department of Surgery, Campinas State University, Avenida Vital Brasil 251, Caixa Postal 6142, Cidade Universitária Zeferino Vaz, 13083-888 Campinas, SP, Brazil.
Juliano César
JC
Moro
Division of Urology, Department of Surgery, Campinas State University, Avenida Vital Brasil 251, Caixa Postal 6142, Cidade Universitária Zeferino Vaz, 13083-888 Campinas, SP, Brazil.
Fábio
F
Coltro Neto
Division of Urology, Department of Surgery, Campinas State University, Avenida Vital Brasil 251, Caixa Postal 6142, Cidade Universitária Zeferino Vaz, 13083-888 Campinas, SP, Brazil.
Ricardo
R
Miyaoka
Division of Urology, Department of Surgery, Campinas State University, Avenida Vital Brasil 251, Caixa Postal 6142, Cidade Universitária Zeferino Vaz, 13083-888 Campinas, SP, Brazil.
Adriano
A
Fregonesi
Nuclear Medicine Division, Department of Radiology, Campinas State University, Avenida Vital Brasil 251, Caixa Postal 6142, Cidade Universitária Zeferino Vaz, 13083-888 Campinas, SP, Brazil.
Mariana da Cunha Lopes
Mda C
de Lima
Nuclear Medicine Division, Department of Radiology, Campinas State University, Avenida Vital Brasil 251, Caixa Postal 6142, Cidade Universitária Zeferino Vaz, 13083-888 Campinas, SP, Brazil.
Celso Darío
CD
Ramos