CETP Gene may be Associated with Advanced Age-Related Macular Degeneration in the Chinese Population.
OBJECTIVE
This study aims to investigate whether variations in LIPC, CETP, ABCA1 and LPL, which are involved in high-density lipoprotein (HDL) metabolism, are associated with advanced age-related macular degeneration (AMD) in the Chinese population.


METHODS
A total of 119 Chinese patients with advanced AMD and 99 control individuals were recruited. Genomic DNA was extracted from peripheral blood leukocytes. Genotypes of seven single nucleotide polymorphisms (SNPs) including rs1061170 and rs1410996 in CFH, rs10490924 in HTRA1, rs10468017 in LIPC, rs3764261 in CETP, rs1883025 in ABCA1 and rs12678919 near LPL were determined by polymerase chain reaction (PCR) followed by allele-specific restriction enzyme digestion or SNaPshot. Unconditional logistic regression analyses were performed to generate a risk predictive model.


RESULTS
We observed the frequency of allele A of rs3764261 in CETP to be significantly lower in advanced AMD after Bonferroni correction (15.5% in patients with AMD and 20.7% in controls; OR = 0.49, 95% CI: 0.29-0.85; p = 0.011). Furthermore, we found that it was also associated with reduced risk of both unilateral AMD (OR = 0.52, 95% CI: 0.28-0.98; p = 0.043) and bilateral AMD (OR = 0.45, 95% CI: 0.22-0.91; p = 0.026). Rs10468017 in LIPC, rs12678919 near LPL and rs1883025 in ABCA1 were not found to be associated with advanced AMD (all p > 0.05).


CONCLUSIONS
Our data suggested that the allele A in rs3764261 in CETP gene may be associated with a decreased risk of advanced AMD in Chinese population.
a Molecular Medicine Research Center, State Key Laboratory of Biotherapy .|b Department of Ophthalmology , West China Hospital, Sichuan University , Chengdu , China , and.
Dan
D
Wang
a Molecular Medicine Research Center, State Key Laboratory of Biotherapy .|b Department of Ophthalmology , West China Hospital, Sichuan University , Chengdu , China , and.
Jie
J
Zhou
a Molecular Medicine Research Center, State Key Laboratory of Biotherapy .
Xiaoming
X
Hou
c Department of Ophthalmology , University of California , San Diego , CA , USA.
Duy H
DH
Nguyen
a Molecular Medicine Research Center, State Key Laboratory of Biotherapy .
Guiqun
G
Cao
a Molecular Medicine Research Center, State Key Laboratory of Biotherapy .
Gen
G
Li
b Department of Ophthalmology , West China Hospital, Sichuan University , Chengdu , China , and.
Guoxian
G
Qiu
a Molecular Medicine Research Center, State Key Laboratory of Biotherapy .|c Department of Ophthalmology , University of California , San Diego , CA , USA.
Kang
K
Zhang
b Department of Ophthalmology , West China Hospital, Sichuan University , Chengdu , China , and.
Ming
M
Zhang
a Molecular Medicine Research Center, State Key Laboratory of Biotherapy .
Zhiguang
Z
Su
D064286:ATP Binding Cassette Transporter 1 / Q000235:genetics; D000368:Aged; D044466:Asian People / Q000235:genetics*; D002681:China / Q000453:epidemiology; D053480:Cholesterol Ester Transfer Proteins / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D060005:Genotyping Techniques; D006801:Humans; D008049:Lipase / Q000235:genetics; D008071:Lipoprotein Lipase / Q000235:genetics; D008268:Macular Degeneration / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide
C577836:ABCA1 protein, human; D064286:ATP Binding Cassette Transporter 1; C506642:CETP protein, human; D053480:Cholesterol Ester Transfer Proteins; C504173:LIPC protein, human; D008049:Lipase; C548261:LPL protein, human; D008071:Lipoprotein Lipase
Age-related macular degeneration; CETP; HDL; genetic polymorphism