A case-control study between interleukin-10 gene variants and periodontal disease in dogs.
Periodontal disease (PD) refers to a group of inflammatory diseases that affect the periodontium, the organ which surrounds and supports the teeth. PD is a highly prevalent disease with a multifactorial etiology and, in humans the individual susceptibility is known to be strongly determined by genetic factors. Several candidate genes have been studied, namely genes related with molecules involved in the inflammatory response. Interleukin-10 (IL-10) is a cytokine with important anti-inflammatory and immunomodulatory roles, and several studies indicate an association between IL10 polymorphisms and PD. In dogs, an important animal model in periodontology, PD is also a highly prevalent naturally occurring disease, and only now are emerging the first studies evaluating the genetic predisposition. In this case-control study, a population of 90 dogs (40 dogs with PD and 50 healthy dogs) was used to study the IL10 gene, and seven new genetic variations in this gene were identified. No statistically significant differences were detected in genotype and allele frequencies of these variations between the PD cases and control groups. Nevertheless, one of the variations (IL10/2_g.285G>A) leads to an amino acid change (glycine to arginine) in the putative signal peptide, being predicted a potential influence on IL-10 protein functionality. Further investigations are important to clarify the biological importance of these new findings. The knowledge of these genetic determinants can help to understand properly the complex causal pathways of PD, with important clinical implications.
Centre of Genomics and Biotechnology-Institute for Biotechnology and Bioengineering (CGB-IBB), University of Trás-os-Montes e Alto Douro (UTAD), P.O. Box 1013, 5001-801 Vila Real, Portugal; Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes e Alto Douro (UTAD), P.O. Box 1013, 5001-801 Vila Real, Portugal. Electronic address: cmralb@hotmail.com.
Carlos
C
Albuquerque
Centre of Genomics and Biotechnology-Institute for Biotechnology and Bioengineering (CGB-IBB), University of Trás-os-Montes e Alto Douro (UTAD), P.O. Box 1013, 5001-801 Vila Real, Portugal; Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes e Alto Douro (UTAD), P.O. Box 1013, 5001-801 Vila Real, Portugal.
Francisco
F
Morinha
Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes e Alto Douro (UTAD), P.O. Box 1013, 5001-801 Vila Real, Portugal; 3B's Research Group: Biomaterials, Biodegradables and Biomimetics, University of Minho, Avepark, Taipas, 4806-909 Guimarães, Portugal.
João
J
Requicha
Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes e Alto Douro (UTAD), P.O. Box 1013, 5001-801 Vila Real, Portugal; 3B's Research Group: Biomaterials, Biodegradables and Biomimetics, University of Minho, Avepark, Taipas, 4806-909 Guimarães, Portugal; ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal.
Isabel
I
Dias
Centre of Genomics and Biotechnology-Institute for Biotechnology and Bioengineering (CGB-IBB), University of Trás-os-Montes e Alto Douro (UTAD), P.O. Box 1013, 5001-801 Vila Real, Portugal.
Henrique
H
Guedes-Pinto
Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes e Alto Douro (UTAD), P.O. Box 1013, 5001-801 Vila Real, Portugal; 3B's Research Group: Biomaterials, Biodegradables and Biomimetics, University of Minho, Avepark, Taipas, 4806-909 Guimarães, Portugal; ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal.
Carlos
C
Viegas
Centre of Genomics and Biotechnology-Institute for Biotechnology and Bioengineering (CGB-IBB), University of Trás-os-Montes e Alto Douro (UTAD), P.O. Box 1013, 5001-801 Vila Real, Portugal; Department of Genetics and Biotechnology, Life and Environmental Sciences School, University of Trás-os-Montes e Alto Douro (UTAD), P.O. Box 1013, 5001-801 Vila Real, Portugal.
Estela
E
Bastos
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D016022:Case-Control Studies; D004285:Dogs / Q000235:genetics*; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D005838:Genotype; D016753:Interleukin-10 / Q000235:genetics*; D008969:Molecular Sequence Data; D010510:Periodontal Diseases / Q000235:genetics* / Q000662:veterinary*; D010519:Periodontium / Q000473:pathology; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA / Q000662:veterinary
D016753:Interleukin-10
Animal model; Dog; Genetic variations; IL10; Interleukin-10; Periodontal disease