Members of the uncultured bacterial candidate division WWE1 are implicated in anaerobic digestion of cellulose.
Clones of the WWE1 (Waste Water of Evry 1) candidate division were retrieved during the exploration of the bacterial diversity of an anaerobic mesophilic (35 ± 0.5°C) digester. In order to investigate the metabolic function of WWE1 members, a 16S rRNA gene -based stable isotope probing (SIP) method was used. Eighty-seven percent of 16S r rRNA gene sequences affiliated to WWE1 candidate division were retrieved in a clone library obtained after polymerase chain reaction (PCR) amplification of enriched DNA fraction from anaerobic municipal solid waste samples incubated with (13) C-cellulose, at the end of the incubation (day 63) using a Pla46F-1390R primer pair. The design of a specific WWE1 probe associated with the fluorescence in situ hybridization (FISH) technique corroborated the abundant representation of WWE1 members in our (13) C-cellulose incubations. Secondary ion mass spectrometry-in situ hybridization (SIMSISH) using an iodine-labeled oligonucleotide probe combined with high-resolution nanometer-scale SIMS (NanoSIMS) observation confirmed the isotopic enrichment of members of WWE1 candidate division. The (13) C apparent isotopic composition of hybridized WWE1 cells reached the value of about 40% early during the cellulose degradation process, suggesting that these bacteria play a role either in an extracellular cellulose hydrolysis process and/or in the uptake fermentation products.
Irstea, UR HBAN, 1 rue Pierre-Gilles de Gennes CS 10030, F-92761, Antony Cedex, France; CEA/Genoscope CNS 2, rue Gaston Crémieux, Evry, 91000, France; University of Evry-Val d'Essonne, Evry, 91057, France; UR04CNSTN01 "Medical and Agricultural Applications of Nuclear Techniques", CNSTN, Sidi Thabet, Ariana, 2020, Tunisia; Faculté des Sciences de Bizerte, Laboratoire de Biochimie et Biologie Moléculaire, 03/UR/0902, Bizerte, Tunisia.
Rim Driss
RD
Limam
Rakia
R
Chouari
Laurent
L
Mazéas
Ting-Di
TD
Wu
Tianlun
T
Li
Julien
J
Grossin-Debattista
Jean-Luc
JL
Guerquin-Kern
Mouldi
M
Saidi
Ahmed
A
Landoulsi
Abdelghani
A
Sghir
Théodore
T
Bouchez
D000693:Anaerobiosis; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D058448:Biota; D002247:Carbon Isotopes / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D017404:In Situ Hybridization, Fluorescence; D007553:Isotope Labeling; D062065:Wastewater / Q000382:microbiology*
D002247:Carbon Isotopes; D002482:Cellulose; D062065:Wastewater
Cellulose anaerobic digestion; FISH; SIMSISH; SIP; WWE1 candidate division