Low-level laser therapy inhibits bronchoconstriction, Th2 inflammation and airway remodeling in allergic asthma.
Low-level laser therapy (LLLT) controls bronchial hyperresponsiveness (BHR) associated with increased RhoA expression as well as pro-inflammatory mediators associated with NF-kB in acute lung inflammation. Herein, we explore if LLLT can reduce both BHR and Th2 cytokines in allergic asthma. Mice were studied for bronchial reactivity and lung inflammation after antigen challenge. BHR was measured through dose-response curves to acetylcholine. Some animals were pretreated with a RhoA inhibitor before the antigen. LLLT (660 nm, 30 mW and 5.4 J) was applied on the skin over the right upper bronchus and two irradiation protocols were used. Reduction of BHR post LLLT coincided with lower RhoA expression in bronchial muscle as well as reduction in eosinophils and eotaxin. LLLT also diminished ICAM expression and Th2 cytokines as well as signal transducer and activator of transduction 6 (STAT6) levels in lungs from challenged mice. Our results demonstrated that LLLT reduced BHR via RhoA and lessened allergic lung inflammation via STAT6.
Nove de Julho University - UNINOVE, Laboratory of Pulmonary and Exercise Immunology - LABPEI, Rua Vergueiro 239/245, PO Box 01504-001, São Paulo, SP, Brazil.
Vanessa R
VR
Silva
Department of Science and Technology, Federal University of São Paulo, PO Box 12231-280, São José dos Campos, SP, Brazil.
P
P
Marcondes
Nove de Julho University - UNINOVE, Laboratory of Pulmonary and Exercise Immunology - LABPEI, Rua Vergueiro 239/245, PO Box 01504-001, São Paulo, SP, Brazil.
M
M
Silva
Institute of Biomedical Engineering, Unicastelo, PO Box 12247-016, São José dos Campos, SP, Brazil.
Antonio B
AB
Villaverde
Laboratory of Immunopharmacology, IOC, FIOCRUZ, PO Box 21045-900, Rio de Janeiro, RJ, Brazil.
Hugo C
HC
Castro-Faria-Neto
Nove de Julho University - UNINOVE, Laboratory of Pulmonary and Exercise Immunology - LABPEI, Rua Vergueiro 239/245, PO Box 01504-001, São Paulo, SP, Brazil.
Rodolfo P
RP
Vieira
Department of Science and Technology, Federal University of São Paulo, PO Box 12231-280, São José dos Campos, SP, Brazil. Electronic address: flavio.aimbire@unifesp.br.
Flavio
F
Aimbire
Nove de Julho University - UNINOVE, Laboratory of Pulmonary and Exercise Immunology - LABPEI, Rua Vergueiro 239/245, PO Box 01504-001, São Paulo, SP, Brazil.
Ana Paula L
AP
de Oliveira
D056151:Airway Remodeling / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects*; D000577:Amides / Q000494:pharmacology; D000818:Animals; D001249:Asthma / Q000188:drug therapy / Q000503:physiopathology / Q000532:radiotherapy*; D001980:Bronchi / Q000187:drug effects / Q000503:physiopathology / Q000528:radiation effects; D016535:Bronchial Hyperreactivity / Q000188:drug therapy / Q000503:physiopathology / Q000532:radiotherapy; D016084:Bronchoconstriction / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects*; D016207:Cytokines / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006967:Hypersensitivity / Q000188:drug therapy / Q000503:physiopathology / Q000532:radiotherapy*; D028022:Low-Level Light Therapy; D008168:Lung / Q000187:drug effects / Q000503:physiopathology / Q000528:radiation effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009130:Muscle, Smooth / Q000187:drug effects / Q000503:physiopathology / Q000528:radiation effects; D010047:Ovalbumin / Q000009:adverse effects; D011014:Pneumonia / Q000188:drug therapy / Q000503:physiopathology / Q000532:radiotherapy; D011725:Pyridines / Q000494:pharmacology; D050800:STAT6 Transcription Factor / Q000378:metabolism; D020741:rho GTP-Binding Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D020742:rhoA GTP-Binding Protein
D000577:Amides; D016207:Cytokines; D004791:Enzyme Inhibitors; D011725:Pyridines; D050800:STAT6 Transcription Factor; C494100:Stat6 protein, mouse; C108830:Y 27632; D010047:Ovalbumin; C526380:RhoA protein, mouse; D020741:rho GTP-Binding Proteins; D020742:rhoA GTP-Binding Protein
Airway hyperresponsiveness; Allergy; Eosinophils; Laser therapy; RhoA; STAT6