Serological diagnosis with recombinant N antigen for hantavirus infection.
Hantaviruses are causative agents of two rodent-borne zoonoses, hemorrhagic fever with renal syndrome (HFRS) and nephropathia epidemica (NE) in the Old World and hantavirus pulmonary syndrome (HPS) in the New World. Serological examinations to detect hantavirus antibodies have been most widely used for surveillance among humans and rodent reservoirs. Here, we will review antigenic structure of nucleocapsid (N) protein of hantaviruses and application of recombinant N protein as diagnostic antigen for screening and serotyping.
Department of Microbiology, Graduate School of Medicine, Hokkaido University, Kita-ku, Kita-15, Nishi-7, Sapporo 060-8638, Japan.
Kumiko
K
Yoshimatsu
Department of Microbiology, Graduate School of Medicine, Hokkaido University, Kita-ku, Kita-15, Nishi-7, Sapporo 060-8638, Japan. Electronic address: j_arika@med.hokudai.ac.jp.
Jiro
J
Arikawa
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000097:blood*; D036022:Capsid Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D002846:Chromatography, Affinity; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006476:Orthohantavirus / Q000145:classification / Q000235:genetics / Q000276:immunology*; D018778:Hantavirus Infections / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D006801:Humans; D009500:Neutralization Tests; D010802:Phylogeny; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D014758:Viral Core Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D011994:Recombinant Proteins; D014758:Viral Core Proteins; C111782:nucleocapsid protein, Hantaan virus
Alternative neutralization test; Immunochoromatography