Radioactive in situ hybridization to detect gene expression in skeletal tissue sections.
In situ hybridization (ISH) using RNA probes is a valuable technique to characterize gene expression patterns in animal tissues. It provides valuable spatial information about gene expression. Compared to the nonradioactive alternatives,(35)S radioactive ISH generally provides higher sensitivity. Here, we describe the procedure for(35)S ISH on paraffin sections from the skeletal tissues of mouse embryos.
Baylor College of Medicine, Houston, TX, USA.
Kyu Sang
KS
Joeng
Washington University School of Medicine, St. Louis, MO, USA.
Jenna
J
Regan
Washington University School of Medicine, St. Louis, MO, USA.
Fanxin
F
Long
D000818:Animals; D001345:Autoradiography; D001842:Bone and Bones / Q000378:metabolism*; D015870:Gene Expression; D017403:In Situ Hybridization / Q000379:methods*; D007553:Isotope Labeling; D051379:Mice; D015347:RNA Probes
D015347:RNA Probes