Plasma concentration of platelet-derived microparticles is related to painful vaso-occlusive phenotype severity in sickle cell anemia.
High plasma level of microparticles (MPs) deriving mainly from erythrocytes and platelets has been detected in sickle cell anemia (SCA) patients. Flow cytometry was used to determine the concentration of MPs in two groups of SCA patients exhibiting marked differences in painful vaso-occlusive crisis rates [a non-severe group (n = 17) and a severe group (n = 12)], and in a control group composed of healthy subjects (n = 20). A 3- to 4-fold increase of total MP plasma concentration was detected in SCA patients. Higher platelet-derived MPs concentration was detected in the severe SCA group while erythrocyte-derived MPs concentration was increased in the non-severe SCA patient group only. Our results suggest that plasma concentration of MPs shed by platelets is a biomarker of the vaso-occlusive phenotype-related severity.
Institut National de la Santé et de la Recherche Médicale, U665, Pointe-à-Pitre, Guadeloupe, France ; Université Antilles-Guyane, Pointe-à-Pitre, France.
Danitza
D
Nebor
Department of Basic Medical Sciences (Physiology Section), University of the West Indies, Kingston, Jamaica.
Andre
A
Bowers
Institut National de la Santé et de la Recherche Médicale, U665, Pointe-à-Pitre, Guadeloupe, France ; Université Antilles-Guyane, Pointe-à-Pitre, France ; Laboratory of Excellence GR-Ex, Pointe-à-Pitre, Guadeloupe, France ; Laboratory ACTES, University of the French West Indies, Guadeloupe, France ; CAribbean network of REsearchers on Sickle cell disease and Thalassemia, Pointe-à-Pitre, Guadeloupe, France ; Institut Universitaire de France, Paris, France.
Philippe
P
Connes
Institut National de la Santé et de la Recherche Médicale, U665, Pointe-à-Pitre, Guadeloupe, France ; Université Antilles-Guyane, Pointe-à-Pitre, France ; Laboratory of Excellence GR-Ex, Pointe-à-Pitre, Guadeloupe, France ; CAribbean network of REsearchers on Sickle cell disease and Thalassemia, Pointe-à-Pitre, Guadeloupe, France.
Marie-Dominique
MD
Hardy-Dessources
CAribbean network of REsearchers on Sickle cell disease and Thalassemia, Pointe-à-Pitre, Guadeloupe, France ; Sickle Cell Unit, Tropical Medicine Research Institute, University of the West Indies, Kingston, Jamaica.
Jennifer
J
Knight-Madden
Sickle Cell Unit, Tropical Medicine Research Institute, University of the West Indies, Kingston, Jamaica.
Vanessa
V
Cumming
CAribbean network of REsearchers on Sickle cell disease and Thalassemia, Pointe-à-Pitre, Guadeloupe, France ; Sickle Cell Unit, Tropical Medicine Research Institute, University of the West Indies, Kingston, Jamaica.
Marvin
M
Reid
Institut National de la Santé et de la Recherche Médicale, U665, Pointe-à-Pitre, Guadeloupe, France ; Université Antilles-Guyane, Pointe-à-Pitre, France ; Laboratory of Excellence GR-Ex, Pointe-à-Pitre, Guadeloupe, France ; CAribbean network of REsearchers on Sickle cell disease and Thalassemia, Pointe-à-Pitre, Guadeloupe, France.
Marc
M
Romana
D000293:Adolescent; D000328:Adult; D000755:Anemia, Sickle Cell / Q000097:blood / Q000150:complications / Q000175:diagnosis* / Q000473:pathology; D015415:Biomarkers / Q000097:blood; D001792:Blood Platelets / Q000737:chemistry* / Q000473:pathology; D055252:Cell-Derived Microparticles / Q000378:metabolism* / Q000473:pathology; D004912:Erythrocytes / Q000737:chemistry* / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D010146:Pain / Q000097:blood / Q000150:complications / Q000175:diagnosis* / Q000473:pathology; D010316:Particle Size; D010641:Phenotype; D012720:Severity of Illness Index
D015415:Biomarkers