Additive benefits of chondroitin sulfate and oriented tethered epidermal growth factor for vascular smooth muscle cell survival.
An anti-apoptotic coating combining chondroitin sulfate (CS) and coiled-coil-based tethering of epidermal growth factor (EGF) is designed for vascular applications. The oriented tethering strategy enables to reach higher EGF surface densities compared to the commonly used random covalent grafting, while using much lower concentrations of EGF during incubation. It also significantly improves vascular smooth muscle cell (VSMC) survival and resistance to apoptosis in serum-free conditions. The comparison of CS and low-fouling carboxymethylated dextran as a sublayer for growth factors highlights the tremendous benefit of CS thanks to its selective protein resistance and good cell adhesion properties. This approach can be tuned by capturing other growth factors on CS through coiled-coil interactions.
Research Centre, Centre Hospitalier de l'Université de Montréal (CRCHUM), 900 rue Saint Denis, Montreal, QC, Canada, H2X 0A9; Department of Mechanical Engineering, École de technologie supérieure (ÉTS), 1100 boul. Notre-Dame Ouest, Montréal, QC, Canada H3C 1K3.
Pauline
P
Lequoy
Benoît
B
Liberelle
Gregory
G
De Crescenzo
Sophie
S
Lerouge
D000704:Analysis of Variance; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002809:Chondroitin Sulfates / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects*; D032389:Myocytes, Smooth Muscle / Q000187:drug effects*; D051381:Rats
D004815:Epidermal Growth Factor; D002809:Chondroitin Sulfates
EGF; VSMC survival; biomaterials; chondroitin sulfate; oriented immobilization