Arabidopsis flowering locus D influences systemic-acquired-resistance- induced expression and histone modifications of WRKY genes.
A plant that is in part infected by a pathogen is more resistant throughout its whole body to subsequent infections--a phenomenon known as systemic acquired resistance (SAR). Mobile signals are synthesized at the site of infection and distributed throughout the plant through vascular tissues. Mechanism of SAR development subsequent to reaching the mobile signal in the distal tissue is largely unknown. Recently we showed that flowering locus D (FLD) gene of Arabidopsis thaliana is required in the distal tissue to activate SAR. FLD codes for a homologue of human-lysine-specific histone demethylase. Here we show that FLD function is required for priming (SAR induced elevated expression during challenge inoculation) of WRKY29 and WRKY6 genes. FLD also differentially influences basal and SAR-induced expression of WRKY38, WRKY65 and WRKY53 genes. In addition, we also show that FLD partly localizes in nucleus and influences histone modifications at the promoters of WRKY29 and WRKY6 genes. The results altogether indicate to the possibility of FLD's involvement in epigenetic regulation of SAR.
School of Life Sciences, Jawaharlal Nehru University, New Delhi 110 067, India.
Vijayata
V
Singh
Shweta
S
Roy
Deepjyoti
D
Singh
Ashis Kumar
AK
Nandi
D017360:Arabidopsis / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000378:metabolism*; D047369:Chromatin Immunoprecipitation; D060467:Disease Resistance / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D049452:Green Fluorescent Proteins; D006655:Histone Deacetylases / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D026161:MADS Domain Proteins / Q000378:metabolism*; D010935:Plant Diseases / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000378:metabolism*
D029681:Arabidopsis Proteins; D006657:Histones; D026161:MADS Domain Proteins; D014157:Transcription Factors; C446285:WRKY6 protein, Arabidopsis; D049452:Green Fluorescent Proteins; C479923:flowering locus D protein, Arabidopsis; D006655:Histone Deacetylases