Nitrate and nitrite as 'in vivo' quenchers of chlorophyll fluorescence in blue-green algae.
The effect of nitrate and nitrite on long-term chlorophyll fluorescence has been studied in filamentous blue-green algae. Cells grown autotrophically with nitrate as nitrogen source show, under argon atmosphere, a high level of fluorescence. The addition of either nitrete or nitrite induces a significant fluorescence quenching, but, whereas in the case of nitrite no previous treatment is required, in the case of nitrate the cells have to be sonicated or treated with Triton X-100 in advance without destroying their cellular integrity. DCMU Abbreviations: BQ, p-benzoquinone; DCMU, 3-(3, 4-dichlorophenyl)-1, 1-dimethylurea; FCCP, carbonylcyanide 4-trifluoromethoxyphenylhydrazone; FeCy, potassium ferricyanide. strongly inhibits the quenching of fluorescence caused by nitrate or nitrite. Using cells grown with ammonia, a nutritional repressor of the two enzymes of the nitrate-reducing system, the fluorescence quenching observed in either case becomes negligible. These results clearly indicate that both nitrate and nitrite can physiologically act as primary Hill reagents in photosynthesis in blue-green algae.
Departmento de Bioquímica, Facultad de Biología y C.S.I.C., Universidad de Sevilla, Apdo. 1095, Sevilla, Spain.
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Rivas
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