A mouse model for endometrioid ovarian cancer arising from the distal oviduct.
Ovarian cancer is the deadliest gynecological malignancy in Western countries. Early detection, however, is hampered by the fact that the origin of ovarian cancer remains unclear. Knowing that in a high percentage of endometrioid ovarian cancers Wnt/β-catenin signaling is activated, and in view of the hypothesis that ovarian cancer may originate from the distal oviduct, we studied mice in which Wnt/β-catenin signaling was activated in Müllerian duct-derived tissues. Conditional adenomatous polyposis coli (Apc) knockout mice were used to study the activation of Wnt/β-catenin signaling in Müllerian duct-derived organs. These Pgr(Cre/+);Apc(ex15lox/lox) mice (n = 44) were sacrificed at 10, 20, 40 and 80 weeks and uterus, oviduct, ovaries and surrounding fat tissues were assessed using immunohistochemistry. Using nuclear β-catenin staining, Wnt/β-catenin signaling activation was confirmed in the entire epithelium of the adult Müllerian duct (fimbriae, oviduct and endometrium), but was absent in ovarian surface epithelium cells (OSEs). Besides endometrial hyperplasia, in 87.2% of mice intraepithelial lesions of the distal oviduct were found, whereas OSEs remained unaffected. In addition, 62.5% of mice developed tumors in the distal and fimbrial part of the oviduct. In the ovaries, mainly at young age, in 16.3% of mice, simple epithelial cysts were noted, which developed further into endometrioid ovarian tumors, resembling human endometrioid ovarian cancer (27.9% of mice). Next to this, locoregional growth in the utero-ovarian ligament was also shown. Here, for the first time, mutations (activation of Wnt/β-catenin) in the distal oviduct result in precursor lesions that develop into ovarian tumors, resembling human endometrioid ovarian cancer.
Department of Obstetrics and Gynecology, Erasmus University Medical Center Rotterdam, Rotterdam, The Netherlands.
Paul H
PH
van der Horst
Marten
M
van der Zee
Claudia
C
Heijmans-Antonissen
Yundan
Y
Jia
Francesco J
FJ
DeMayo
John P
JP
Lydon
Carolien H M
CH
van Deurzen
Patricia C
PC
Ewing
Curt W
CW
Burger
Leen J
LJ
Blok
D011125:Adenomatous Polyposis Coli / Q000235:genetics; D000818:Animals; D018269:Carcinoma, Endometrioid / Q000235:genetics / Q000473:pathology*; D000077216:Carcinoma, Ovarian Epithelial; D004195:Disease Models, Animal; D004714:Endometrial Hyperplasia / Q000473:pathology; D004717:Endometrium / Q000473:pathology; D004847:Epithelial Cells / Q000473:pathology; D005187:Fallopian Tubes / Q000473:pathology*; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009374:Neoplasms, Experimental / Q000235:genetics / Q000473:pathology; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics / Q000473:pathology*; D010051:Ovarian Neoplasms / Q000235:genetics / Q000473:pathology*; D051153:Wnt Proteins / Q000378:metabolism; D060449:Wnt Signaling Pathway / Q000235:genetics*; D051176:beta Catenin / Q000378:metabolism
D051153:Wnt Proteins; D051176:beta Catenin
APC; Wnt/β-catenin signaling; endometrioid ovarian cancer; oviduct; tubal intraepithelial lesions