TGF-β favors bone marrow-derived dendritic cells to acquire tolerogenic properties.
Dendritic cells (DCs) are the most powerful antigen-presenting cells that have an important role in the immunity and immune tolerance. Transforming growth factor β (TGF-β) is a pleiotropic cytokine widely expressing in various tissues and cells, which regulates cellular proliferation, differentiation and apoptosis of several immune cells and is considered to be a key factor in inducing immune tolerance. The effect of TGF-β on DCs is very complex. In this study, we further investigated the effect of TGF-β on inducing immune tolerance of DCs. DCs were differentiated from mice bone marrow cells in the absence or presence of TGF-β. The phenotype as well as function was studied in detail. We found that TGF-β limited the expression of CD40, CD83, CD86 and MHCII in DCs, increased CD45RB and indoleamine 2, 3-dioxygenase (IDO) expression in DCs, promoted IL-10 and limited IL-12 secretion by DCs. Moreover, TGF-β increased the endocytosis ability of DCs and limited the ability of DCs in activating T cells. These results suggest that TGF-β affects the immunity of DCs and enhances their tolerogenicity.
Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Antiinflammatory and Immune Medicine, Anhui Medical University, Ministry of Education , Hefei 230032 , China.
Sha-Sha
SS
Song
Ping-Fan
PF
Yuan
Jing-Yu
JY
Chen
Jing-Jing
JJ
Fu
Hua-Xun
HX
Wu
J T
JT
Lu
W
W
Wei
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D004705:Endocytosis / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D007108:Immune Tolerance / Q000235:genetics*; D016130:Immunophenotyping; D016753:Interleukin-10 / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D016212:Transforming Growth Factor beta / Q000378:metabolism* / Q000494:pharmacology
D000954:Antigens, Surface; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D018664:Interleukin-12