Coupling of tyrosine deprotonation and axial ligand exchange in nitrocytochrome c.
Here we report a spectroscopic, electrochemical and computational study of cytochrome c showing that nitration of Tyr74 induces Tyr deprotonation, which is coupled to Met/Lys axial ligand exchange, and results in concomitant gain of peroxidatic activity at physiological pH.
Departamento de Química Inorgánica, Analítica y Química Física and INQUIMAE, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires-CONICET, Argentina. dhmurgida@qi.fcen.uba.ar.
Daiana A
DA
Capdevila
Damián
D
Álvarez-Paggi
María A
MA
Castro
Verónica
V
Tórtora
Verónica
V
Demicheli
Darío A
DA
Estrín
Rafael
R
Radi
Daniel H
DH
Murgida
D003198:Computer Simulation; D045304:Cytochromes c / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D008958:Models, Molecular; D009566:Nitrates / Q000737:chemistry*; D010084:Oxidation-Reduction; D011522:Protons; D013816:Thermodynamics; D014443:Tyrosine / Q000737:chemistry*
D008024:Ligands; D009566:Nitrates; D011522:Protons; D014443:Tyrosine; D045304:Cytochromes c