Nanomolar pyrophosphate detection in water and in a self-assembled hydrogel of a simple terpyridine-Zn2+ complex.
A simple terpyridine-Zn(II) complex is shown to act as an efficient and highly selective fluorescent sensor for pyrophosphate in water at physiological pH. The sensor complex showed an unprecedented fluorescence response (∼500 fold increase) and a record nanomolar sensitivity (detectable fluorescent response at 20 nM and LOD ∼ 0.8 nM). It has successfully been used to stain and record confocal fluorescence microscopy images of HeLa cells. Moreover, the complex was found to self-assemble into a hydrogel which was subsequently used to coat disposable paper strips for easy, low-cost detection of pyrophosphate.
Department of Chemistry, Nanoscience Center, University of Jyväskylä , P.O. Box 35, 40014 Jyväskylä, Finland.
Sandip
S
Bhowmik
Biswa Nath
BN
Ghosh
Varpu
V
Marjomäki
Kari
K
Rissanen
D011756:Diphosphates / Q000032:analysis*; D020100:Hydrogels; D057230:Limit of Detection; D008856:Microscopy, Fluorescence; D011725:Pyridines / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D014867:Water / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry*
D011756:Diphosphates; D020100:Hydrogels; D011725:Pyridines; D014867:Water; D015032:Zinc; C023666:pyridine