Synthesis of antibacterial TiO2/PLGA composite biofilms.
This study developed a TiO2/PLGA [poly(lactic-co-glycolic acid)] composite biomaterial, which possesses antibacterial properties but is biocompatible, for artificial dressing applications. A sol-gel method was used for the preparation of the nano TiO2 powder with anatase phase. Several concentration ratios of TiO2 versus PLGA were analyzed to optimize the disinfection efficiency of the composite biomaterial. The antibacterial activity of the fabricated TiO2/PLGA composite was measured against Staphylococcus aureus and Escherichia coli. To evaluate the feasibility of the biomaterial on wound healing in vitro, human keratinocytes (HaCaTs), fibroblasts (L929s), and bovine carotid artery endothelial cells (BECs) were seeded on the TiO2/PLGA composite biofilms. To investigate the histological effect of the biocompatible biofilm in vivo, a rat subcutaneous implantation was performed. Our results show that TiO2/PLGA composite biofilms containing 10% TiO2 nanoparticles have an effective antibacterial property, a good survival rate on HaCaTs and L929s, and relative safe stability in tissue implantation.


BACKGROUND
This study reports the development of titanium dioxide-polylactic-co-glycolic acid composite biofilms, which possess antibacterial properties and are biocompatible for dressing applications, as demonstrated in a model system.
Graduate Institute of Biomedical Engineering, National Chung-Hsing University, Taichung, Taiwan. Electronic address: sally82876756@hotmail.com.
Jau-Yi
JY
Wu
PhD Program in Tissue Engineering and Regenerative Medicine, National Chung-Hsing University, Taichung, Taiwan. Electronic address: fayeshin2@hotmail.com.
Ching-Wen
CW
Li
Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, Taiwan. Electronic address: chtsai1@dragon.nchu.edu.tw.
Ching-Hsiu
CH
Tsai
Department of Cosmeceutics, China Medical University, Taichung, Taiwan. Electronic address: cwchou@mail.cmu.edu.tw.
Chih-Wei
CW
Chou
Comprehensive Breast Cancer Center, Changhua Christian Hospital, Changhua, Taiwan. Electronic address: 1886@cch.org.tw.
Dar-Ren
DR
Chen
Graduate Institute of Biomedical Engineering, National Chung-Hsing University, Taichung, Taiwan; PhD Program in Tissue Engineering and Regenerative Medicine, National Chung-Hsing University, Taichung, Taiwan; Department of Mechanical Engineering, National Chung-Hsing University, Taichung, Taiwan. Electronic address: gjwang@dragon.nchu.edu.tw.
Gou-Jen
GJ
Wang
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D019344:Lactic Acid / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D014025:Titanium / Q000737:chemistry*
D000900:Anti-Bacterial Agents; C009495:titanium dioxide; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D014025:Titanium
Antibacterial material; Artificial dressing; TiO(2)/PLGA composite biofilm; Titanium dioxide