Fluorescence microscopy study of Rap1 subcellular localization.
The Ras-related GTPase Rap has been implicated in multiple cellular functions. In particular, Rap1 is a crucial regulator of both inside-out integrin activation and outside-in E-cadherin-mediated signaling. Thus, Rap1 was proposed as a fundamental regulator of the cross talk between cadherins and integrins. We provide microscopic techniques to study subcellular localization of Rap1 protein in the crosstalk between integrins and cadherins.
Department of Clinical and Biological Sciences, University of Torino, Torino, Italy.
Luca
L
Goitre
Valentina
V
Cutano
Saverio Francesco
SF
Retta
D015820:Cadherins / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation; D005455:Fluorescent Antibody Technique; D016023:Integrins / Q000378:metabolism; D042541:Intracellular Space / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000379:methods*; D021381:Protein Transport; D020827:rap1 GTP-Binding Proteins / Q000378:metabolism*
D015820:Cadherins; D016023:Integrins; D020827:rap1 GTP-Binding Proteins