Solid-phase incorporation of an ATRP initiator for polymer-DNA biohybrids.
The combination of polymers with nucleic acids leads to materials with significantly advanced properties. To obviate the necessity and complexity of conjugating two macromolecules, a polymer initiator is described that can be directly covalently linked to DNA during solid-phase synthesis. Polymer can then be grown from the DNA bound initiator, both in solution after the DNA-initiator is released from the solid support as well as directly on the solid support, simplifying purification. The resulting polymer-DNA hybrids were examined by chromatography and fluorescence methods that attested to the integrity of hybrids and the DNA. The ability to use DNA-based supports expands the range of readily available molecules that can be used with the initiator, as exemplified by direct synthesis of a biotin polymer hybrid on solid-support. This method expands the accessibility and range of advanced polymer biohybrid materials.
Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213 (USA) http://www.cmu.edu/maty/index.html.
Saadyah E
SE
Averick
Sourav K
SK
Dey
Debasish
D
Grahacharya
Krzysztof
K
Matyjaszewski
Subha R
SR
Das
D001710:Biotin / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*
D011108:Polymers; D001710:Biotin; D004247:DNA
DNA; atom-transfer radical polymerization; bioconjugates; polymers