Vertical nanowire probes for intracellular signaling of living cells.
The single living cell action potential was measured in an intracellular mode by using a vertical nanoelectrode. For intracellular interfacing, Si nanowires were vertically grown in a controlled manner, and optimum conditions, such as diameter, length, and nanowire density, were determined by culturing cells on the nanowires. Vertical nanowire probes were then fabricated with a complimentary metal-oxide-semiconductor (CMOS) process including sequential deposition of the passivation and electrode layers on the nanowires, and a subsequent partial etching process. The fabricated nanowire probes had an approximately 60-nm diameter and were intracellular. These probes interfaced with a GH3 cell and measured the spontaneous action potential. It successfully measured the action potential, which rapidly reached a steady state with average peak amplitude of approximately 10 mV, duration of approximately 140 ms, and period of 0.9 Hz.
Ki-Young
KY
Lee
Ilsoo
I
Kim
So-Eun
SE
Kim
Du-Won
DW
Jeong
Ju-Jin
JJ
Kim
Hyewhon
H
Rhim
Jae-Pyeong
JP
Ahn
Seung-Han
SH
Park
Department of Materials Science and Engineering, Yonsei University, Seoul 120-749, Republic of Korea. hjc@yonsei.ac.kr.
Heon-Jin
HJ
Choi