JMJD6 regulates ERα methylation on arginine.
ERα functions are tightly controlled by numerous post-translational modifications including arginine methylation, which is required to mediate the extranuclear functions of the receptor. We report that upon oestrogenic stimulation, JMJD6, the only arginine demethylase described so far, interacts with and regulates methylated ERα (metERα) function. Moreover, by combining the silencing of JMJD6 with demethylation assays, we show that metERα is a new substrate for JMJD6. We propose that the demethylase activity of JMJD6 is a decisive regulator of the rapid physiological responses to oestrogen.
Université de Lyon, F-69000 Lyon, France ; Université Lyon 1, F-69000 Lyon, France ; Inserm U1052, Centre de Recherche en Cancérologie de Lyon, F-69000 Lyon, France ; CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, F-69000 Lyon, France ; Equipe Labellisée « La Ligue »
Coralie
C
Poulard
Université de Lyon, F-69000 Lyon, France ; Université Lyon 1, F-69000 Lyon, France ; Inserm U1052, Centre de Recherche en Cancérologie de Lyon, F-69000 Lyon, France ; CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, F-69000 Lyon, France ; Equipe Labellisée « La Ligue »
Juliette
J
Rambaud
Lebanese University, Faculty of Sciences, Doctoral School of Sciences and Technology, PRASE, Hadath, Lebanon.
Nader
N
Hussein
Université de Lyon, F-69000 Lyon, France ; Université Lyon 1, F-69000 Lyon, France ; Inserm U1052, Centre de Recherche en Cancérologie de Lyon, F-69000 Lyon, France ; CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, F-69000 Lyon, France ; Equipe Labellisée « La Ligue »
Laura
L
Corbo
Université de Lyon, F-69000 Lyon, France ; Université Lyon 1, F-69000 Lyon, France ; Inserm U1052, Centre de Recherche en Cancérologie de Lyon, F-69000 Lyon, France ; CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, F-69000 Lyon, France ; Equipe Labellisée « La Ligue »
Muriel
M
Le Romancer
D001120:Arginine / Q000737:chemistry*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism; D002846:Chromatography, Affinity; D019175:DNA Methylation; D047628:Estrogen Receptor alpha / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007091:Image Processing, Computer-Assisted; D047468:Immunoprecipitation; D056484:Jumonji Domain-Containing Histone Demethylases / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014407:Tumor Cells, Cultured
C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D001120:Arginine; C498707:JMJD6 protein, human; D056484:Jumonji Domain-Containing Histone Demethylases