Expression Analysis of Sugarcane Aquaporin Genes under Water Deficit.
The present work is a pioneer study specifically addressing the aquaporin transcripts in sugarcane transcriptomes. Representatives of the four aquaporin subfamilies (PIP, TIP, SIP, and NIP), already described for higher plants, were identified. Forty-two distinct aquaporin isoforms were expressed in four HT-SuperSAGE libraries from sugarcane roots of drought-tolerant and -sensitive genotypes, respectively. At least 10 different potential aquaporin isoform targets and their respective unitags were considered to be promising for future studies and especially for the development of molecular markers for plant breeding. From those 10 isoforms, four (SoPIP2-4, SoPIP2-6, OsPIP2-4, and SsPIP1-1) showed distinct responses towards drought, with divergent expressions between the bulks from tolerant and sensitive genotypes, when they were compared under normal and stress conditions. Two targets (SsPIP1-1 and SoPIP1-3/PIP1-4) were selected for validation via RT-qPCR and their expression patterns as detected by HT-SuperSAGE were confirmed. The employed validation strategy revealed that different genotypes share the same tolerant or sensitive phenotype, respectively, but may use different routes for stress acclimation, indicating the aquaporin transcription in sugarcane to be potentially genotype-specific.
Department of Genetics, Federal University of Pernambuco (UFPE), 50670-901 Recife, PE, Brazil.
Manassés Daniel
MD
da Silva
Department of Genetics, Federal University of Pernambuco (UFPE), 50670-901 Recife, PE, Brazil.
Roberta Lane de Oliveira
RL
Silva
Department of Genetics, Federal University of Pernambuco (UFPE), 50670-901 Recife, PE, Brazil.
José Ribamar
JR
Costa Ferreira Neto
Biotechnology Division, Sugarcane Technology Center (CTC), 13400-970 Piracicaba, SP, Brazil.
Ana Carolina Ribeiro
AC
Guimarães
Biotechnology Division, Sugarcane Technology Center (CTC), 13400-970 Piracicaba, SP, Brazil.
Daniela Truffi
DT
Veiga
Biotechnology Division, Sugarcane Technology Center (CTC), 13400-970 Piracicaba, SP, Brazil.
Sabrina Moutinho
SM
Chabregas
Biotechnology Division, Sugarcane Technology Center (CTC), 13400-970 Piracicaba, SP, Brazil.
William Lee
WL
Burnquist
Institute of Molecular Biosciences, Frankfurt University, 60438 Frankfurt am Main, Germany.
Günter
G
Kahl
Department of Genetics, Federal University of Pernambuco (UFPE), 50670-901 Recife, PE, Brazil.
Ana Maria
AM
Benko-Iseppon
Department of Genetics, Federal University of Pernambuco (UFPE), 50670-901 Recife, PE, Brazil.
Ederson Akio
EA
Kido