Mapping of the gynoecy in bitter gourd (Momordica charantia) using RAD-seq analysis.
Momordica charantia is a monoecious plant of the Cucurbitaceae family that has both male and female unisexual flowers. Its unique gynoecious line, OHB61-5, is essential as a maternal parent in the production of F1 cultivars. To identify the DNA markers for this gynoecy, a RAD-seq (restriction-associated DNA tag sequencing) analysis was employed to reveal genome-wide DNA polymorphisms and to genotype the F2 progeny from a cross between OHB61-5 and a monoecious line. Based on a RAD-seq analysis of F2 individuals, a linkage map was constructed using 552 co-dominant markers. In addition, after analyzing the pooled genomic DNA from monoecious or gynoecious F2 plants, several SNP loci that are genetically linked to gynoecy were identified. GTFL-1, the closest SNP locus to the putative gynoecious locus, was converted to a conventional DNA marker using invader assay technology, which is applicable to the marker-assisted selection of gynoecy in M. charantia breeding.
Gene Research Center, Shinshu University, Ueda, Nagano, Japan.
Hideo
H
Matsumura
Okinawa Prefectural Agriculture Research Center, Itoman, Okinawa, Japan.
Norimichi
N
Miyagi
Okinawa Prefectural Agriculture Research Center, Itoman, Okinawa, Japan.
Naoki
N
Taniai
Gene Research Center, Shinshu University, Ueda, Nagano, Japan.
Mai
M
Fukushima
Okinawa Prefectural Agriculture Research Center, Itoman, Okinawa, Japan.
Kazuhiko
K
Tarora
Okinawa Prefectural Agriculture Research Center, Itoman, Okinawa, Japan.
Ayano
A
Shudo
Okinawa Prefectural Agriculture Research Center, Itoman, Okinawa, Japan.
Naoya
N
Urasaki
D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods; D008040:Genetic Linkage / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics*; D005838:Genotype; D008969:Molecular Sequence Data; D029777:Momordica charantia / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods
D005819:Genetic Markers