Tunable swelling of polyelectrolyte multilayers in cell culture media for modulating NIH-3T3 cells adhesion.
For polyelectrolyte multilayers (PEMs) assembled by the layer-by-layer (LbL) assembly technique, their nanostructure and properties can be governed by many parameters during the building process. Here, it was demonstrated that the swelling of the PEMs containing poly(diallyldimethylammonium chloride) (PDDA) and poly(sodium 4-styrenesulfonate) (PSS) in cell culture media could be tuned with changing supporting salt solutions during the assembly process. Importantly, the influence of the PEMs assembled in different salt solutions on NIH-3T3 cell adhesion was observable. Specifically, the cells could possess a higher affinity for the films assembled in low salt concentration (i.e. 0.15M NaCl) or no salt, the poorly swelling films in cell culture media, which was manifested by the large cell spreading area and focal adhesions. In contrast, those were assembled in higher salt concentration, highly swelling films in cell culture media, were less attractive for the fibroblasts. As a result, the cell adhesion behaviors may be manipulated by tailoring the physicochemical properties of the films, which could be performed by changing the assembly conditions such as supporting salt concentration. Such a finding might promise a great potential in designing desired biomaterials for tissue engineering and regenerative medicine.
Key Laboratory of Life-Organic Analysis, School of Chemistry and Chemical Engineering, Qufu Normal University, 57 Jingxuan West Road, Qufu Shandong, 273165, China; Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, School of Chemistry and Chemical Engineering, Qufu Normal University, 57 Jingxuan West Road, Qufu Shandong, 273165, China.
Wei
W
Qi
Peng
P
Cai
Wenjing
W
Yuan
Hua
H
Wang
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D022001:Focal Adhesions / Q000378:metabolism*; D008567:Membranes, Artificial; D051379:Mice; D041681:NIH 3T3 Cells; D011095:Polyethylenes / Q000737:chemistry / Q000494:pharmacology; D011137:Polystyrenes / Q000737:chemistry / Q000494:pharmacology; D000644:Quaternary Ammonium Compounds / Q000737:chemistry / Q000494:pharmacology; D044968:Regenerative Medicine / Q000379:methods; D023822:Tissue Engineering / Q000379:methods
D003470:Culture Media; D008567:Membranes, Artificial; D011095:Polyethylenes; D011137:Polystyrenes; D000644:Quaternary Ammonium Compounds; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; C003321:polystyrene sulfonic acid
cell adhesion; layer-by-layer assembly; polyelectrolyte multilayer film; supporting salt concentration; swelling