Error rate for imputation from the Illumina BovineSNP50 chip to the Illumina BovineHD chip.
BACKGROUND
Imputation of genotypes from low-density to higher density chips is a cost-effective method to obtain high-density genotypes for many animals, based on genotypes of only a relatively small subset of animals (reference population) on the high-density chip. Several factors influence the accuracy of imputation and our objective was to investigate the effects of the size of the reference population used for imputation and of the imputation method used and its parameters. Imputation of genotypes was carried out from 50,000 (moderate-density) to 777,000 (high-density) SNPs (single nucleotide polymorphisms).


METHODS
The effect of reference population size was studied in two datasets: one with 548 and one with 1289 Holstein animals, genotyped with the Illumina BovineHD chip (777 k SNPs). A third dataset included the 548 animals genotyped with the 777 k SNP chip and 2200 animals genotyped with the Illumina BovineSNP50 chip. In each dataset, 60 animals were chosen as validation animals, for which all high-density genotypes were masked, except for the Illumina BovineSNP50 markers. Imputation was studied in a subset of six chromosomes, using the imputation software programs Beagle and DAGPHASE.


RESULTS
Imputation with DAGPHASE and Beagle resulted in 1.91% and 0.87% allelic imputation error rates in the dataset with 548 high-density genotypes, when scale and shift parameters were 2.0 and 0.1, and 1.0 and 0.0, respectively. When Beagle was used alone, the imputation error rate was 0.67%. If the information obtained by Beagle was subsequently used in DAGPHASE, imputation error rates were slightly higher (0.71%). When 2200 moderate-density genotypes were added and Beagle was used alone, imputation error rates were slightly lower (0.64%). The least imputation errors were obtained with Beagle in the reference set with 1289 high-density genotypes (0.41%).


CONCLUSIONS
For imputation of genotypes from the 50 k to the 777 k SNP chip, Beagle gave the lowest allelic imputation error rates. Imputation error rates decreased with increasing size of the reference population. For applications for which computing time is limiting, DAGPHASE using information from Beagle can be considered as an alternative, since it reduces computation time and increases imputation error rates only slightly.
CRV BV, P,O, Box 454, 6800 AL Arnhem, The Netherlands. chris.schrooten@crv4all.com.
Chris
C
Schrooten
Romain
R
Dassonneville
Vincent
V
Ducrocq
Rasmus F
RF
Brøndum
Mogens S
MS
Lund
Jun
J
Chen
Zengting
Z
Liu
Oscar
O
González-Recio
Juan
J
Pena
Tom
T
Druet
D000483:Alleles; D000818:Animals; D002417:Cattle / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D060005:Genotyping Techniques / Q000295:instrumentation*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D020641:Polymorphism, Single Nucleotide