Effect of sauchinone, a lignan from Saururus chinensis, on bacterial phagocytosis by macrophages.
AMP-activated protein kinase (AMPK) plays an important role in inflammation in various cells and increases the phagocytic ability of macrophages. In this study, we found that sauchinone increased the phosphorylation of AMPK and acetyl-CoA carboxylase (ACC), a downstream target of AMPK, in mouse peritoneal macrophages. Sauchinone increased macrophage phagocytosis of fluorescent Escherichia coli, which was blocked by compound C, an AMPK inhibitor. Sauchinone also increased the phosphorylation of p38 mitogen activated protein kinase (MAPK) in cultured macrophages in a concentration-dependent fashion, which was not blocked by compound C. However, the increase of sauchinone-induced phagocytosis was prevented by SB203580. An inhibitor of the upstream kinase TGF-beta-activated kinase (TAK1), (5z)-7-oxozeaenol, abolished the phosphorylation of ACC and p38 MAPK. Systemic administration of sauchinone to mice led to increased phosphorylation of AMPK and p38 MAPK in the lung, and enhanced phagocytosis of fluorescent E. coli in bronchoalveolar lavage fluid as compared with control mice. These results suggest sauchinone to be a useful adjunctive treatment for bacterial infection.
Center for Creative Biomedical Scientists at Chonnam National University, Republic of Korea; Research Institute of Medical Sciences, Chonnam National University, Republic of Korea.
Kyung-Min
KM
Jeong
Departments of Anesthesiology and Pain Medicine, Chonnam National University Medical School, 5 Hak-dong, Gwangju 501-746, Republic of Korea; Center for Creative Biomedical Scientists at Chonnam National University, Republic of Korea.
Jeong-Il
JI
Choi
Departments of Anesthesiology and Pain Medicine, Chonnam National University Medical School, 5 Hak-dong, Gwangju 501-746, Republic of Korea.
Seong-Heon
SH
Lee
Departments of Anesthesiology and Pain Medicine, Chonnam National University Medical School, 5 Hak-dong, Gwangju 501-746, Republic of Korea.
Hyun-Jung
HJ
Lee
College of Pharmacy, Yeungnam University, Gyongsan 712-749, Republic of Korea.
Jong-Keun
JK
Son
Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine, Exporo 483, Yusung-gu, Daejeon 305-811, Republic of Korea.
Chang-Seob
CS
Seo
Departments of Anesthesiology and Pain Medicine, Chonnam National University Medical School, 5 Hak-dong, Gwangju 501-746, Republic of Korea.
Seung-Won
SW
Song
Departments of Anesthesiology and Pain Medicine, Chonnam National University Medical School, 5 Hak-dong, Gwangju 501-746, Republic of Korea; Center for Creative Biomedical Scientists at Chonnam National University, Republic of Korea.
Sang-Hyun
SH
Kwak
Departments of Anesthesiology and Pain Medicine, Chonnam National University Medical School, 5 Hak-dong, Gwangju 501-746, Republic of Korea; Center for Creative Biomedical Scientists at Chonnam National University, Republic of Korea. Electronic address: nextphil2@jnu.ac.kr.
Hong-Beom
HB
Bae
D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D000818:Animals; D001578:Benzopyrans / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D002478:Cells, Cultured; D004149:Dioxoles / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000276:immunology; D048168:Escherichia coli K12 / Q000276:immunology; D005434:Flow Cytometry; D017705:Lignans / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects*; D032134:Saururaceae / Q000737:chemistry*
D001578:Benzopyrans; D004149:Dioxoles; D017705:Lignans; C410958:sauchinone; D055372:AMP-Activated Protein Kinases; D020929:Mitogen-Activated Protein Kinase Kinases
AMP-activated protein kinase; Bacteria; Phagocytosis; Sauchinone; p38 Mitogen activated protein kinase