Analysis of the IGHV region in Burkitt's lymphomas supports a germinal center origin and a role for superantigens in lymphomagenesis.
The analysis of immunoglobulin heavy chain variable (IGHV) region may disclose the influence of antigens in Burkitt's lymphomas (BL). IGHV sequences from 38 patients and 35 cell lines were analyzed. IGHV3 subset genes were the most used and IGHV4-34 gene was overrepresented. IGHV genes were mutated in 98.6% of the cases, 36% acquired potential glycosylation sites, and in 52% somatic-hypermutation-process was ongoing. Binding motifs for superantigens like Staphylococcal protein A and carbohydrate I/i were preserved in 89% of the cases. IGHV analysis of BL cells supports a germinal center origin and points toward a role for superantigens in lymphomagenesis.
Laboratory of Experimental Hematology, Department of Hematology, Vall d'Hebron University Hospital, Universitat Autonòma de Barcelona, Barcelona, Spain.
Maria Joao
MJ
Baptista
Laboratory of Experimental Hematology, Department of Hematology, Vall d'Hebron University Hospital, Universitat Autonòma de Barcelona, Barcelona, Spain.
Eva
E
Calpe
Department of Pathology, Hospital Clinic, IDIBAPS, University of Barcelona, Barcelona, Spain.
Eva
E
Fernandez
Department of Pathology, Hospital Clinic, IDIBAPS, University of Barcelona, Barcelona, Spain.
Lluis
L
Colomo
Department of Pediatric Oncology, Hospital Sant Joan de Déu, University of Barcelona, Barcelona, Spain.
Teresa Marta
TM
Cardesa-Salzmann
Laboratory of Experimental Hematology, Department of Hematology, Vall d'Hebron University Hospital, Universitat Autonòma de Barcelona, Barcelona, Spain.
Pau
P
Abrisqueta
Laboratory of Experimental Hematology, Department of Hematology, Vall d'Hebron University Hospital, Universitat Autonòma de Barcelona, Barcelona, Spain. Electronic address: fbosch@vhebron.net.
Francesc
F
Bosch
Laboratory of Experimental Hematology, Department of Hematology, Vall d'Hebron University Hospital, Universitat Autonòma de Barcelona, Barcelona, Spain.
Marta
M
Crespo
D000293:Adolescent; D000328:Adult; D000368:Aged; D002051:Burkitt Lymphoma / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D004252:DNA Mutational Analysis; D005260:Female; D018858:Germinal Center / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D018089:Superantigens / Q000502:physiology*
D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D018089:Superantigens
BL; Glycosylation; IGHV; Intraclonal diversity; SHM; Superantigens