Resveratrol increases brown adipose tissue thermogenesis markers by increasing SIRT1 and energy expenditure and decreasing fat accumulation in adipose tissue of mice fed a standard diet.
OBJECTIVE
Adipose tissue is central to the regulation of energy balance. Two functionally different fat pads are present in mammals: white adipose tissue, the primary site of triglyceride storage, and brown adipose tissue (BAT), which is specialized in heat production. In this context, new strategies capable of modulating the development and function of white and BAT become relevant. In the present study, we analyzed the influence of resveratrol (sirtuin activator) on energy balance and the expression of thermogenesis markers.


METHODS
Mice were divided into two groups: standard diet (ST) and standard diet plus resveratrol (ST + RSV).


RESULTS
After 2 months of treatment, ST + RSV mice presented significantly decreased fat accumulation in adipose tissue, with diminished total cholesterol and glucose plasma levels. Additionally, increased oxygen consumption was observed in ST + RSV group. Analyses of mRNA of thermogenesis-related genes showed significant increase in UCP1, SIRT1, PTEN and BMP-7 expression in BAT.


CONCLUSIONS
Our data suggest that improved metabolism produced by oral administration of resveratrol is, at least in part, associated with increased thermogenesis followed by high expression of UCP1 and SIRT1, which can mediate higher energy expenditure and decreased fat accumulation in adipose tissue.
Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros (UNIMONTES), Montes Claros, Minas Gerais, Brazil.
João Marcus Oliveira
JM
Andrade
Alessandra Caroline Montes
AC
Frade
Juliana Bohnen
JB
Guimarães
Kátia Michelle
KM
Freitas
Miriam Teresa Paz
MT
Lopes
André Luiz Sena
AL
Guimarães
Alfredo Maurício Batista
AM
de Paula
Cândido Celso
CC
Coimbra
Sérgio Henrique Sousa
SH
Santos
D002001:Adipose Tissue, Brown / Q000187:drug effects* / Q000378:metabolism; D052436:Adipose Tissue, White / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D055419:Bone Morphogenetic Protein 7 / Q000235:genetics / Q000378:metabolism; D004032:Diet; D004734:Energy Metabolism / Q000187:drug effects*; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism; D000077185:Resveratrol; D056564:Sirtuin 1 / Q000235:genetics / Q000378:metabolism*; D013267:Stilbenes / Q000494:pharmacology*; D022722:Thermogenesis / Q000187:drug effects*; D000071256:Uncoupling Protein 1
D055419:Bone Morphogenetic Protein 7; D007473:Ion Channels; D024101:Mitochondrial Proteins; D013267:Stilbenes; C000604372:Ucp1 protein, mouse; D000071256:Uncoupling Protein 1; C525820:bmp7 protein, mouse; D051059:PTEN Phosphohydrolase; C487757:Pten protein, mouse; C470545:Sirt1 protein, mouse; D056564:Sirtuin 1; D000077185:Resveratrol