Large-scale axonal reorganization of inhibitory neurons following retinal lesions.
The functional properties of adult cortical neurons are subject to alterations in sensory experience. Retinal lesions lead to remapping of cortical topography in the region of primary visual cortex representing the lesioned part of the retina, the lesion projection zone (LPZ), with receptive fields shifting to the intact parts of the retina. Neurons within the LPZ receive strengthened input from the surrounding region by growth of the plexus of excitatory long-range horizontal connections. Here, by combining cell type-specific labeling with a genetically engineered recombinant adeno-associated virus and in vivo two-photon microscopy in adult macaques, we showed that the remapping was also associated with alterations in the axonal arbors of inhibitory neurons, which underwent a parallel process of pruning and growth. The axons of inhibitory neurons located within the LPZ extended across the LPZ border, suggesting a mechanism by which new excitatory input arising from the peri-LPZ is balanced by reciprocal inhibition arising from the LPZ.
Laboratory of Neurobiology, The Rockefeller University, New York, New York 10065.
Sally A
SA
Marik
Homare
H
Yamahachi
Stephan
S
Meyer zum Alten Borgloh
Charles D
CD
Gilbert
D000818:Animals; D001369:Axons / Q000378:metabolism / Q000473:pathology*; D001931:Brain Mapping; D000229:Dependovirus / Q000235:genetics / Q000502:physiology; D005968:Glutamate Decarboxylase / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008252:Macaca fascicularis; D008297:Male; D009433:Neural Inhibition / Q000502:physiology*; D009473:Neuronal Plasticity / Q000502:physiology*; D062308:Optogenetics; D012164:Retinal Diseases / Q000473:pathology* / Q000503:physiopathology; D011984:Sensory Receptor Cells / Q000473:pathology* / Q000502:physiology; D014161:Transduction, Genetic; D014793:Visual Cortex / Q000473:pathology*; D014794:Visual Fields / Q000502:physiology; D014795:Visual Pathways / Q000502:physiology
C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D005968:Glutamate Decarboxylase; C401141:glutamate decarboxylase 2