Xpert GBS assay for rapid detection of group B streptococcus in gastric fluid samples from newborns.
The Xpert GBS real-time PCR assay was applied to gastric fluid samples from 143 newborns, and it detected group B streptococcus (GBS) within 1 h for 16 (11.2%) cases, while microscopic examination detected only 2 cases. The sensitivity and specificity of the Xpert GBS were 80% and 100%, respectively, with regard to 20 cases of GBS colonization or infection. Concordance of Xpert GBS results versus culture was 92.3%. This test detects in a timely manner newborns at risk for invasive GBS disease.
APHP, Groupe Hospitalier Saint Louis-Lariboisière-Fernand Widal, Service de Bactériologie-Virologie, Paris, France.
Christelle
C
Jost
Béatrice
B
Bercot
Hervé
H
Jacquier
Laurent
L
Raskine
Emmanuel
E
Barranger
Geneviève
G
Mouchnino
Emmanuelle
E
Cambau
D001431:Bacteriological Techniques / Q000379:methods*; D005260:Female; D005750:Gastric Juice / Q000382:microbiology*; D006801:Humans; D007231:Infant, Newborn; D008853:Microscopy / Q000379:methods; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D011247:Pregnancy; D011446:Prospective Studies; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013290:Streptococcal Infections / Q000175:diagnosis*; D013292:Streptococcus agalactiae / Q000302:isolation & purification*; D013995:Time