Effects of high-impact mechanical loading on synovial cell cultures.
Cartilage metabolism in response to mechanical loading is an important subject in sports science and medicine. In animal studies high-impact exercise is known to stimulate bone adaptation and increase bone mass. However, mechanical impacts potentially induce tissue swelling and occasionally degradation of connective tissues in synovium and articular cartilage. These detrimental outcomes should be properly evaluated clinically and biochemically. Using two synovial cell cultures derived from normal and rheumatic tissues, we examined the biochemical effects of impulsive mechanical loads on expression and activities of influential proteolytic enzymes in joints, matrix metalloproteinases (MMPs), and their natural inhibitors, tissue inhibitors of metalloproteinases (TIMPs). The molecular analysis demonstrates that an impact factor (Im ), the ratio of the maximum force to weight, served as a good indicator for assessment of the inflammatory responses. The results showed that high impact above Im = 40 to 80 elevated not only expression but also enzymatic activities of MMPs. Key PointsHigh-impact loading elevated expression and activities of MMPs in synovial cell cultures.The impact factor was used to define in vitro intensity of high-impact loading.
Department of Anatomy and Cell Biology, Indiana University - Purdue University Indianapolis , Indianapolis, USA.
Irene
I
Sun
Biomedical Engineering Program, Indiana University - Purdue University Indianapolis , Indianapolis, USA ; Department of Biomedical Engineering, Purdue University , West Lafayette, USA.
Yunlong
Y
Liu
Department of Anatomy and Cell Biology, Indiana University - Purdue University Indianapolis , Indianapolis, USA.
Shigeo M
SM
Tanaka
Biomedical Engineering Program, Indiana University - Purdue University Indianapolis , Indianapolis, USA.
Chung W
CW
Lee
Department of Anatomy and Cell Biology, Indiana University - Purdue University Indianapolis , Indianapolis, USA ; Biomedical Engineering Program, Indiana University - Purdue University Indianapolis , Indianapolis, USA.
Hui Bin
HB
Sun
Department of Anatomy and Cell Biology, Indiana University - Purdue University Indianapolis , Indianapolis, USA ; Biomedical Engineering Program, Indiana University - Purdue University Indianapolis , Indianapolis, USA ; Department of Biomedical Engineering, Purdue University , West Lafayette, USA.
Hiroki
H
Yokota
Impulsive factor; MMP; rheumatoid arthritis; synovium