[Effect of Taohong Siwu decoction on angiogenesis of medicine-induced incomplete-abortion in early pregnancy rats and expressions of Ang-1, Ang-2 and Tie-2].
OBJECTIVE
To observe the effect of Taohong Siwu decoction (THSWD) on micro-vascular density (MVD) in rat uterus, the content of angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2) in serum, and the expression of tyrosine kinasa receptor (Tie-2) in uterus.


METHODS
Early pregnancy rats were intragastrically administrated with misoprostol (100 microg x kg(-1)) and mifepristong (8.3 mg x kg(-1)) to established the incomplete-abortion model. The incomplete-abortion rats were randomly divided into the model group (the same volume of distilled water), the positive control group (at the daily dose of 4.3 g x kg(-1) Motherwort Particles), and THSWD-treated groups (at the daily dose of 18.0, 9.0 and 4.5 g x kg(-1)). Pregnant rats were taken as the control group (the same volume of distilled water). After the successive oral administration for 7 days, blood was collected from aorta abdominalis, and rat uterine tissues were collected. The content of serum Ang-1 and Ang-2 were detected by ELISA; And the levels of Tie-2 and MVD in uterine tissues were detected by SP immunohistochemistry.


RESULTS
THSWD remarkably increased the levels of MVD in uterus of medicine-induced abortion rats, the content of Ang-1 and Ang-2 in serum, and the expression of Tie-2 in uterine tissues.


CONCLUSIONS
THSWD has the effect in markedly promoting angiogenesis in incomplete-abortion rats. Its mechanism may be related to the regulation of concentrations of Ang-1 and Ang-2 in serum and Tie-2 in uterine tissues.
School of Pharmacy, Anhui University of Chinese Medicine, Key Laboratory of Modern Traditional Chinese Medicines of Anhui Province, Hefei 230038, China. 260266215@qq.com
Jie
J
Liang
School of Pharmacy, Anhui University of Chinese Medicine, Key Laboratory of Modern Traditional Chinese Medicines of Anhui Province, Hefei 230038, China.
Deng-Ke
DK
Yin
School of Pharmacy, Anhui University of Chinese Medicine, Key Laboratory of Modern Traditional Chinese Medicines of Anhui Province, Hefei 230038, China.
Bai-Kun
BK
Li
School of Pharmacy, Anhui University of Chinese Medicine, Key Laboratory of Modern Traditional Chinese Medicines of Anhui Province, Hefei 230038, China.
Zhu-Qing
ZQ
Liu
School of Pharmacy, Anhui University of Chinese Medicine, Key Laboratory of Modern Traditional Chinese Medicines of Anhui Province, Hefei 230038, China.
Shan-Shan
SS
Li
School of Pharmacy, Anhui University of Chinese Medicine, Key Laboratory of Modern Traditional Chinese Medicines of Anhui Province, Hefei 230038, China.
Meng-Xia
MX
Chen
School of Pharmacy, Anhui University of Chinese Medicine, Key Laboratory of Modern Traditional Chinese Medicines of Anhui Province, Hefei 230038, China.
Xiao-Yu
XY
Wang
School of Pharmacy, Anhui University of Chinese Medicine, Key Laboratory of Modern Traditional Chinese Medicines of Anhui Province, Hefei 230038, China.
Dai-Yin
DY
Peng
D000027:Abortion, Incomplete / Q000097:blood / Q000188:drug therapy* / Q000235:genetics*; D042683:Angiopoietin-1 / Q000097:blood / Q000235:genetics*; D042702:Angiopoietin-2 / Q000097:blood / Q000235:genetics*; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D042787:Receptor, TIE-2 / Q000235:genetics* / Q000378:metabolism; D014599:Uterus / Q000098:blood supply* / Q000187:drug effects / Q000378:metabolism
D042683:Angiopoietin-1; D042702:Angiopoietin-2; D004365:Drugs, Chinese Herbal; D042787:Receptor, TIE-2