Easy daylight fabricated hydrogel array for colorimetric DNA analysis.
The fabrication of 3D hydrogel microarrays for DNA analytics that allow simple visual signal readout for on-site applications is described. A convenient one-step polymerization of the hydrogel including in situ capture oligonucleotide immobilization is accomplished by using N,N'-dimethylacrylamide/polyethylene glycol (PEG1900 )-bisacrylamide monomers. The implementation of an acylphosphine-oxide photoinitiator even allows polymerization at daylight, whereas other approaches require exposure with light in the UV-range. This minimizes the risk of UV-caused DNA damages within the capture DNA-strand that could adversely affect the subsequent hybridization step. The porous network of these gel segments allows DNA as well as protein penetration. Thus, the successful in-gel DNA hybridization is monitored by the deposition of silver nanoparticles. These metal particles allow naked eye signal readout.
Leibniz Institute of Photonic Technology e.V., Albert-Einstein-Strasse 9, 07745, Jena, Germany; Institute of Physical Chemistry and Abbe Centre of Photonics, Friedrich-Schiller-University Jena, Helmholtzweg 4, 07743, Jena, Germany.
Antje
A
Beyer
Sibyll
S
Pollok
Albrecht
A
Berg
Karina
K
Weber
Jürgen
J
Popp
D000178:Acrylamides / Q000737:chemistry; D004247:DNA / Q000032:analysis*; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D016062:Porosity; D012834:Silver / Q000737:chemistry*; D014466:Ultraviolet Rays
D000178:Acrylamides; D020100:Hydrogels; D009841:Oligonucleotides; D012834:Silver; D011092:Polyethylene Glycols; D004247:DNA; C099046:N,N-dimethylacrylamide
DNA immobilization; chip-based DNA analysis; daylight photo-polymerization; hydrogel; one-step polymerization