Determination of naproxen in human plasma by GC-MS.
This paper describes a GC-MS method for the determination of naproxen in human plasma. Naproxen and internal standard ibuprofen were extracted from plasma using a liquid-liquid extraction method. Derivatization was carried out using N-methyl-N-(trimethylsilyl)trifluoroacetamide. The calibration curve was linear between the concentration range of 0.10-5.0 μg/mL. Intra- and interday precision values for naproxen in plasma were <5.14, and accuracy (relative error) was better than 4.67%. The extraction recoveries of naproxen from human plasma were between 93.0 and 98.9%. The LOD and LOQ of naproxen were 0.03 and 0.10 μg/mL, respectively. Also, this assay was applied to determine the pharmacokinetic parameters of naproxen in six healthy Turkish volunteers who had been given 220 mg naproxen.
Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, Erzurum, Turkey.
Bilal
B
Yilmaz
Huseyin
H
Sahin
Ali Fuat
AF
Erdem
D000328:Adult; D008401:Gas Chromatography-Mass Spectrometry; D064368:Healthy Volunteers; D006801:Humans; D007052:Ibuprofen / Q000097:blood; D059625:Liquid-Liquid Extraction; D009288:Naproxen / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D012680:Sensitivity and Specificity
D009288:Naproxen; D007052:Ibuprofen
Ibuprofen; Liquid-liquid extraction; Naproxen; Pharmacokinetics; Validation