Luteolin induces apoptosis by activating Fas signaling pathway at the receptor level in laryngeal squamous cell line Hep-2 cells.
Luteolin, a naturally occurring flavonoid, possesses anti-cancer activities against several human cancers, but the exact molecular and biochemical mechanisms of above findings are not very clear, and its activity against head and neck squamous cell carcinoma (HNSCC) is seldom mentioned. In this study, we investigated luteolin against human laryngeal squamous cell line Hep-2 cells, using MTT assay, flow cytometry, Western blot and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. Luteolin inhibited Hep-2 cells proliferation at the inhibitive concentrations of 50% (IC50) near to 50 μM and induced the apoptosis in Hep-2 cells through caspase-3 and caspase-8 activation. Up-regulation of Fas and down-regulation of long form cellular FLICE-like inhibitory protein (c-FLIPL) protein were also involved after luteolin treatment at both protein and mRNA levels. Luteolin could not only inhibit cell proliferation but also induce apoptosis by activating the Fas signaling pathway at the receptor level in laryngeal squamous cell line Hep-2 cells.
Department of Histology and Embryology, Jining Medical University, Shandong Province, People's Republic of China.
Hui
H
Zhang
Xiuguo
X
Li
Yuanyuan
Y
Zhang
Xinyong
X
Luan
D017209:Apoptosis / Q000187:drug effects*; D053446:CASP8 and FADD-Like Apoptosis Regulating Protein / Q000187:drug effects*; D002294:Carcinoma, Squamous Cell; D053148:Caspase 3 / Q000187:drug effects*; D053181:Caspase 8 / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D006258:Head and Neck Neoplasms; D006801:Humans; D007822:Laryngeal Neoplasms; D047311:Luteolin / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*; D000077195:Squamous Cell Carcinoma of Head and Neck; D015854:Up-Regulation; D019014:fas Receptor / Q000187:drug effects*
D053446:CASP8 and FADD-Like Apoptosis Regulating Protein; C491901:FAS protein, human; D019014:fas Receptor; C505407:CASP3 protein, human; C505530:CASP8 protein, human; D053148:Caspase 3; D053181:Caspase 8; D047311:Luteolin