Assay of Ca2+ transport by VDAC1 reconstituted into liposomes.
Ca(2+) permeability mediated by voltage-dependent anion-selective channel protein 1 (VDAC1) can be tested by reconstitution of purified VDAC1 into liposomes. Here, we describe a setup for this membranal system, which has been used to study the transport activity of various transporters, including VDAC1, and allows detection of the passage of molecules across the lipid bilayer. Despite the disadvantage of needing radiolabeled molecules, this system is highly desirable when the transport properties of noncharged molecules and/or active transporters are studied.
Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel.
Danya
D
Ben-Hail
Varda
V
Shoshan-Barmatz
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D008081:Liposomes / Q000378:metabolism*; D011510:Proteolipids / Q000378:metabolism; D051381:Rats; D050995:Voltage-Dependent Anion Channel 1 / Q000378:metabolism
D008081:Liposomes; D011510:Proteolipids; C020485:proteoliposomes; D050995:Voltage-Dependent Anion Channel 1; D002118:Calcium