The clinical value of the quantitative detection of four cancer-testis antigen genes in multiple myeloma.
BACKGROUND
Cancer-testis (CT) antigen genes might promote the progression of multiple myeloma (MM). CT antigens may act as diagnostic and prognostic markers in MM, but their expression levels and clinical implications in this disease are not fully understood. This study measured the expression levels of four CT antigen genes in Chinese patients with MM and explored their clinical implications.


METHODS
Real-time quantitative polymerase chain reaction (qPCR) was used to quantify the expression of MAGE-C1/CT7, MAGE-A3, MAGE-C2/CT10 and SSX-2 mRNA in 256 bone marrow samples from 144 MM patients.


RESULTS
In the newly diagnosed patients, the positive expression rates were 88.5% for MAGE-C1/CT7, 82.1% for MAGE-C2/CT10, 76.9% for MAGE-A3 and 25.6% for SSX-2. The expression levels and the number of co-expressed CT antigens correlated significantly with several clinical indicators, including the percentage of plasma cells infiltrating the bone marrow, abnormal chromosome karyotypes and the clinical course.


CONCLUSIONS
MAGE-C1/CT7, MAGE-A3, MAGE-C2/CT10 and SSX-2 expression levels provide potentially effective clinical indicators for the auxiliary diagnosis and monitoring of treatment efficacy in MM.
Yao
Y
Zhang
Li
L
Bao
Jin
J
Lu
Kai-Yan
KY
Liu
Jin-Lan
JL
Li
Ya-Zhen
YZ
Qin
Huan
H
Chen
Ling-Di
LD
Li
Yuan
Y
Kong
Hong-Xia
HX
Shi
Yue-Yun
YY
Lai
Yan-Rong
YR
Liu
Bin
B
Jiang
Shan-Shan
SS
Chen
Peking University People's Hospital and Institute of Hematology, Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation, No,11 Xi-Zhi-Men South Street, 100044 Beijing, China. xjhrm@medmail.com.cn.
Xiao-Jun
XJ
Huang
Guo-Rui
GR
Ruan
D000951:Antigens, Neoplasm / Q000032:analysis* / Q000235:genetics; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000235:genetics* / Q000473:pathology; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor