Proliferation and adhesion capability of human gingival fibroblasts onto zirconia, lithium disilicate and feldspathic veneering ceramic in vitro.
Human gingival fibroblasts (HGFs) were cultured onto CAD/CAM zirconia (Group A), CAD/CAM zirconia after polishing (Group B), CAD/CAM lithium disilicate after polishing (Group C), and feldspathic ceramic (Group D) to evaluate their proliferation and adhesion potential. After 3 h, HGF adhesion was similar in all groups. Later, HGFs closely adhered to surfaces, particularly onto groups B, C and D, acquiring an elongated shape. Proliferation assay showed no differences in cell viability among the groups after 24 h, while significant increase was shown after 72 h in Groups B and C. After 24 h, similar Collagen I levels were found in all groups, while after 72 h Groups B and C revealed a deep reduction in respect to the 24 h level. In vitro, HGF behavior may reflect variability in soft tissue response to different surface materials for prosthetic restorations, and support that polished zirconia is able to achieve a better integration in vivo in respect to the other materials.
Department of Medical, Oral, and Biotechnological Sciences, University "G. d'Annunzio"
Stefano
S
Tetè
Vincenzo Luca
VL
Zizzari
Bruna
B
Borelli
Marianna
M
De Colli
Susi
S
Zara
Roberto
R
Sorrentino
Antonio
A
Scarano
Enrico
E
Gherlone
Amelia
A
Cataldi
Fernando
F
Zarone
D000538:Aluminum Silicates / Q000737:chemistry; D002448:Cell Adhesion; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D002516:Ceramics / Q000737:chemistry*; D017076:Computer-Aided Design; D003776:Dental Porcelain / Q000737:chemistry; D003801:Dental Veneers; D005347:Fibroblasts / Q000502:physiology*; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D017680:Potassium Compounds / Q000737:chemistry; D013499:Surface Properties; D015040:Zirconium / Q000737:chemistry
D000538:Aluminum Silicates; D017680:Potassium Compounds; C493120:lithia disilicate; D003776:Dental Porcelain; C016447:feldspar; D015040:Zirconium; C028541:zirconium oxide