Simultaneous characterisation of fifty coumarins from the roots of Angelica dahurica by off-line two-dimensional high-performance liquid chromatography coupled with electrospray ionisation tandem mass spectrometry.
BACKGROUND
The root of Angelica dahurica is a traditional Chinese medicine that used for the treatment of headache, toothache, abscess, furunculosis and acne. Coumarins were the major bioactive constituents of A. dahurica, hence it is worthwhile developing a method to simultaneously characterise them, especially those in trace amounts.


OBJECTIVE
To develop an efficient method for the simultaneous characterisation of coumarins in A. dahurica.


METHODS
A method using off-line two-dimensional high-performance liquid chromatography coupled with electrospray tandem mass spectrometry (off-line 2D-HPLC-ESI/MS(n) ) was developed.


RESULTS
In total 50 coumarins, including 32 linear furanocoumarins, 16 bifuranocoumarins and two non-furanocoumarins, were identified from the roots of A. dahurica. The possible MS fragmentations of these coumarins are also proposed.


CONCLUSIONS
The method described here allows rapid and convenient identification of the coumarins in A. dahurica, and may be applied to other herbal medicines containing linear furanocoumarins.
Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 100029, China.
Bin
B
Li
Xu
X
Zhang
Juan
J
Wang
Le
L
Zhang
Bowen
B
Gao
Shepo
S
Shi
Xiaohui
X
Wang
Jun
J
Li
Pengfei
P
Tu
D029969:Angelica / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D011564:Furocoumarins / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D003374:Coumarins; D004365:Drugs, Chinese Herbal; D011564:Furocoumarins
Angelica dahurica; Off-line 2D-HPLC-ESI/MSn; coumarin; furanocoumarins; spirobifuranocoumarin