Effects of renal sympathetic denervation using saline-irrigated radiofrequency ablation catheter on the activity of the renin-angiotensin system and endothelin-1.
BACKGROUND
Excessive activation of the sympathetic nervous system (SNS) and the renin-angiotensin system (RAS) are crucial and interacted closely in the pathogenesis of chronic cardiovascular diseases. This study investigated the effects of renal denervation (RDN) on the RAS.


METHODS
Eight Chinese Kunming dogs underwent bilateral RDN utilizing saline-irrigated radiofrequency ablation catheter. Blood pressure (BP) measurements, blood sampling assays and renal angiography were performed at baseline, 30 min, one month and three months after ablation.


RESULTS
During three months of follow-up, RDN caused a significant and uniform reduction in plasma level of renin, angiotensin II, and endothelin-1(ET-1), with the reduction of -5.7±6.8 (p=0.049), -19.4±19.3 (p=0.025), and -22.4±21 pg/ml (p=0.02) for plasma renin, -10.6±7.2 (p=0.004), -15.9±8.8 (p=0.001), and -15.2±9.6 pg/ml (p=0.003) for plasma angiotensin II, as well as -3.9±3 (p=0.007), -10.8±5 (p<0.001), and -14.6±6.1 pg/ml (p<0.001) for plasma ET-1. RDN utilizing a saline-irrigated catheter also caused a progressive and substantial BP reduction of -19±22/-8±13, -30±13/-13±14, and -36±20/-16±14 mm Hg (p=0.045, p<0.001, and p<0.002 for systolic BP; p=0.14, p=0.036, and p=0.014 for diastolic BP) without ablation-related complications.


CONCLUSIONS
RDN substantially decreased BP and also significantly decreased the plasma levels of RAS and ET-1, which might be implicated in the mechanism of BP reduction by RDN.
Department of Cardiology, The Second Affiliated Hospital of Chongqing Medical University, China.
Jiayi
J
Lu
Department of Cardiology, The Second Affiliated Hospital of Chongqing Medical University, China.
Zhiyu
Z
Ling
Department of Cardiology, The Second Affiliated Hospital of Chongqing Medical University, China.
Weijie
W
Chen
Department of Cardiology, The Second Affiliated Hospital of Chongqing Medical University, China.
Huaan
H
Du
Department of Cardiology, The Second Affiliated Hospital of Chongqing Medical University, China.
Yanping
Y
Xu
Department of Cardiology, The Second Affiliated Hospital of Chongqing Medical University, China.
Jinqi
J
Fan
Department of Cardiology, The Second Affiliated Hospital of Chongqing Medical University, China.
Yi
Y
Long
Department of Cardiology, The Second Affiliated Hospital of Chongqing Medical University, China.
Shaojie
S
Chen
Department of Cardiology, The Second Affiliated Hospital of Chongqing Medical University, China.
Peilin
P
Xiao
Department of Cardiology, The Second Affiliated Hospital of Chongqing Medical University, China.
Zengzhang
Z
Liu
Medizinische Klinik I, Krankenhaus Landshut/Achdorf, Germany.
Bernhard
B
Zrenner
Department of Cardiology, The Second Affiliated Hospital of Chongqing Medical University, China yinyh63@163.com.
Yuehui
Y
Yin
D000792:Angiography; D000804:Angiotensin II / Q000097:blood; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D017115:Catheter Ablation; D003714:Denervation; D004285:Dogs; D019332:Endothelin-1 / Q000097:blood*; D005260:Female; D007668:Kidney / Q000000981:diagnostic imaging / Q000187:drug effects / Q000294:innervation* / Q000503:physiopathology; D008297:Male; D012083:Renin / Q000097:blood; D012084:Renin-Angiotensin System / Q000187:drug effects*; D012965:Sodium Chloride / Q000494:pharmacology*; D013562:Sympathectomy; D013599:Systole / Q000187:drug effects
D019332:Endothelin-1; D000804:Angiotensin II; D012965:Sodium Chloride; D012083:Renin
Renal sympathetic denervation; endothelin-1; renal artery ablation; renin-angiotensin system; saline-irrigated radiofrequency ablation catheter