[Effect of human umbilici mesenchymal stromal cells implantation on the BDNF expression in diabetic foot rats].
OBJECTITVE: To investigate the effect of human umbilici mesenchymal cells (HUMSCs) implantation on the brain derived neurotrophic factor (BDNF) expression in diabetic foot rats.


METHODS
SD rats were divided into three groups (n = 12): normal group, diadetic foot model group and HUMSC treatment group. Diabetic foot model in rats was established, then prepared HUMSC were implanted on the diabetic foot ulcers in rats, and control ones were administrated with saline only. The area of ulceration, sensory function, BDNF expression and its localization were determined by using morphology, physiological function measurement, RT-PCR and immunohistochemistry assay.


RESULTS
Siglificantly decreased area of ulceration in diabetic foot rats of HUMSC implantation group was observed. This was simultaneously companied with the sensory function improvement (P < 0.05). RT-PCR showed that BDNF mRNA expression was significantly up regulated (P < 0.05). BDNF immunstaining was located in epithelia tissue and the protein level of BDNF was markedly increased (P < 0.05).


CONCLUSIONS
HUMSC implantation maybe an effective strategy on the treatment of ulceration in diabetic foot rats, and the possible mechanism may involve in BDNF expression.
Institute of Neuroscience, Kunming Medical University, Kunming 650031, China.
Ning
N
Zhou
Institute of Neurological Disease and Translation Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, China.
Qing-Ping
QP
Wang
Yan-an Hospital Affiliated to Kunming Medical University, Kunming 650051, China.
Xing-Fang
XF
Jin
Yan-an Hospital Affiliated to Kunming Medical University, Kunming 650051, China.
Zong-Liu
ZL
Hou
Yan-an Hospital Affiliated to Kunming Medical University, Kunming 650051, China.
Bao-Kun
BK
Peng
Yan-an Hospital Affiliated to Kunming Medical University, Kunming 650051, China.
Qi-Qin
QQ
Dan
Institute of Neuroscience, Kunming Medical University, Kunming 650031, China.
Tin-Hua
TH
Wang
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000150:complications; D017719:Diabetic Foot / Q000378:metabolism / Q000628:therapy*; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014470:Umbilical Cord / Q000166:cytology*; D014945:Wound Healing / Q000502:physiology
D019208:Brain-Derived Neurotrophic Factor