Proteomic analysis of human-derived cell culture supplements.
OBJECTIVE
Development of cell therapy and advanced therapy medicinal products depends on in vitro expansion of human cells in fetal bovine serum (FBS) supplemented media. Human-derived supplements, such as human serum (huS) and human platelet lysate (hPL), represent suitable alternatives to FBS. Various studies demonstrated that the use of these human alternatives result in comparable or even improved proliferation and expansion ratios.


METHODS
Within this study three human supplement alternatives, huS, hPLP (plasma containing hPL) and hPLN (plasma replaced by saline), were compared by 2D gel electrophoresis, an important tool in proteomic analysis. 2D gel electrophoresis allows the determination of the protein number and the detection of protein changes (decreasing/increasing concentration).


CONCLUSIONS
The comparison of huS, hPLP, and hPLN gels resulted in clearly visible differences in protein pattern, protein number and concentration, particularly when comparing huS with hPL and hPLP with hPLN.
Red Cross Blood Transfusion Service of Upper Austria ; Austrian Cluster for Tissue Regeneration, Linz, Austria.
Jasmin
J
Kemptner
Red Cross Blood Transfusion Service of Upper Austria ; Austrian Cluster for Tissue Regeneration, Linz, Austria.
Karin
K
Witzeneder
Red Cross Blood Transfusion Service of Upper Austria ; Austrian Cluster for Tissue Regeneration, Linz, Austria.
Simone
S
Hennerbichler
Red Cross Blood Transfusion Service of Upper Austria ; Austrian Cluster for Tissue Regeneration, Linz, Austria.
Christian
C
Gabriel
Cell therapy; Fetal calf serum; Human platelet lysate; Human serum; Proteomics