Bio-imaging of hydroxyl radicals in plant cells using the fluorescent molecular probe rhodamine B hydrazide, without any pretreatment.
Rhodamine B hydrazide can be used to detect hydroxyl radicals in plant cells. RBH was easily inserted into plant cells without any pretreatment, and specifically reacted with intracellular hydroxyl radicals produced by antimycin A. RBH will be a powerful tool for detecting hydroxyl radicals in plant cells.
Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan. Electronic address: asanom@gly.oups.ac.jp.
Mamiko
M
Asano
Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
Mitsunobu
M
Doi
Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
Kimiye
K
Baba
Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
Masahiko
M
Taniguchi
Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
Makio
M
Shibano
Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
Satoshi
S
Tanaka
Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
Minoru
M
Sakaguchi
Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
Masanori
M
Takaoka
Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
Masahiko
M
Hirata
Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
Rika
R
Yanagihara
Department of Pharmacy, Oita University Hospital, 1-1 Idaigaoka Hasama-machi, Yuhu, Oita 879-5593, Japan.
Ryosuke
R
Nakahara
Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
Yuko
Y
Hayashi
Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
Takako
T
Yamaguchi
Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
Hitoshi
H
Matsumura
Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
Yoshikazu
Y
Fujita
D005456:Fluorescent Dyes / Q000737:chemistry*; D006834:Hydrazines / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000032:analysis*; D018613:Microscopy, Confocal; D015335:Molecular Probes / Q000737:chemistry; D059828:Plant Cells / Q000737:chemistry*; D012235:Rhodamines / Q000737:chemistry*
D005456:Fluorescent Dyes; D006834:Hydrazines; D015335:Molecular Probes; D012235:Rhodamines; C543681:rhodamine B hydrazide; D017665:Hydroxyl Radical
Hydroxyl radical; Nicotiana tabacum BY-2; Onion epidermal cell; Reactive oxygen species; Rhodamine B hydrazide