Wheat V-H+-ATPase subunit genes significantly affect salt tolerance in Arabidopsis thaliana.
Genes for V-H(+)-ATPase subunits were identified and cloned from the salt-tolerant wheat mutant RH8706-49. Sequences of these genes are highly conserved in plants. Overexpression of these genes in Arabidopsis thaliana improved its salt tolerance, and increased the activities of V-H(+)-ATPase and Na(+)/H(+) exchange, with the largest increase in plants carrying the c subunit of V-H(+)-ATPase. Results from quantitative RT-PCR analysis indicated that the mRNA level of each V-H(+)-ATPase subunit in the Arabidopsis increased under salt stress. Overall, our results suggest that each V-H(+)-ATPase subunit plays a key role in enhancing salt tolerance in plants.
School of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhang, Hebei, China.
Xiaoliang
X
He
College of Life Science of Hebei Normal University, Shijiazhuang, Hebei, China.
Xi
X
Huang
College of Life Science of Hebei Normal University, Shijiazhuang, Hebei, China.
Yinzhu
Y
Shen
College of Life Science of Hebei Normal University, Shijiazhuang, Hebei, China.
Zhanjing
Z
Huang
D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D003001:Cloning, Molecular; D015870:Gene Expression; D010641:Phenotype; D010802:Phylogeny; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified; D021122:Protein Subunits / Q000235:genetics; D055049:Salt Tolerance / Q000235:genetics*; D012965:Sodium Chloride / Q000378:metabolism; D014908:Triticum / Q000201:enzymology; D025262:Vacuolar Proton-Translocating ATPases / Q000096:biosynthesis / Q000235:genetics*
D010940:Plant Proteins; D021122:Protein Subunits; D012965:Sodium Chloride; D025262:Vacuolar Proton-Translocating ATPases