Micropropagation and acclimatization of Stevia rebaudiana Bertoni.
Multiple shoot induction of Stevia rebaudiana Bertoni was studied by node explants that were cultured on solidified MS media and supplemented with 0, 1, 2, 3 and 4 mg L-1 kinetin for 4 weeks. The results showed the maximum amount of multiple shoot induction (9.31+/-4.17 shoots/explant) when cultured on MS media supplemented with 3 mg L-1 kinetin. In vitro shoots were rooted on solidified MS media supplemented with 0, 0.1, 0.5 and 2 mg L-1 Naphthaleneacetic Acid (NAA) for 4 weeks. The highest number of roots (11.18+/-1.34 roots/shoot) was detected on a concentration of 0.1 mg L-1 NAA while the high survival rate (80%) was obtained when the rooted plantlets were transferred to greenhouse conditions.
Biology Program, Faculty of Science and Technology, Chiang Mai Rajabhat University, Chiang Mai, Thailand.
Kittisak
K
Chotikadachanarong
Department of Biology, Chiang Mai University, Chiang Mai, Thailand.
Srisuluk
S
Dheeranupattana
D000064:Acclimatization; D018556:Crops, Agricultural / Q000187:drug effects / Q000254:growth & development*; D007701:Kinetin / Q000494:pharmacology; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots / Q000254:growth & development; D018520:Plant Shoots / Q000254:growth & development; D012639:Seeds / Q000254:growth & development; D036625:Stevia / Q000187:drug effects / Q000254:growth & development*; D013997:Time Factors; D046509:Tissue Culture Techniques
D009280:Naphthaleneacetic Acids; D010937:Plant Growth Regulators; D007701:Kinetin