Enzymes of the glycollate pathway in relation to greening in Euglena gracilis.
The kinetics of chlorophyll formation during the greening of dark-grown Euglena gracilis was accompanied by marked increases in activity of the enzymes of glycollate metabolism; glycollate: DCPIP(1) oxidoreductase and phosphoglycollate phosphatase (E.C. 3.1.3.18.). Inhibitors of protein synthesis indicated a de novo synthesis of these enzymes during the development of the photosynthetic system. The inhibitory effects of chloramphenicol and cycloheximide, together with a non-aqueous localisation of glycollate: DCPIP oxidoreductase in photoautotrophically-grown cells, indicated that this enzyme is synthesized and located in the cytoplasm, while phosphoglycollate phosphatase was synthesized in the chloroplast. Glycollate: DCPIP oxidoreductase did not increase above the low level in heterotrophic cells when exogenous glycollate, in the presence or absence of glucose, was supplied in the dark.
Postgraduate School of Biological Sciences, University of Bradford, Bradford, Yorkshire, England.
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GA
Codd
M J
MJ
Merrett