FasL gene -844T/C mutation of esophageal cancer in South China and its clinical significance.
In this study, we investigated the association between the FasL -844T/C polymorphism and the risk of developing esophageal squamous cell carcinoma (ESCC) in South China. For the investigation, we randomly selected 248 patients suffering from ESCC from Southern China along with 297 healthy individuals as the control group. The relationship between the FasL gene -844T/C SNP and ESCC was studied using PCR-RFLP and immunohistochemistry. The Fas -1377G/A SNP was also selected for investigation to detect whether it interferes with the functional effect of the FasL -844C/T polymorphism in ESCC development. A significant difference in the FasL -844T/C genotypes between the patients and the control group was observed (P<0.05), with those expressing the C allele having a significantly reduced risk of developing ESCC, however elderly patients (>60 years) exhibited a more malignant pathological grade if they were homozygous for the C allele. FasL -844 CC combined with the Fas -1377 G allele is a protective factor against ESCC. Having said this, even though the C allele has a protective effect prior to development of ESCC, once the host does develop the condition the tumour will develop faster and have a higher degree of malignancy than T carriers.
1] Department of thoracic surgery, Zhejiang Cancer Hospital, Hangzhou 310022 [2] Zhejiang Key Laboratory of Diagnosis and Treatment Technology on Thoracic Oncology (Lung and Esophagus) Zhejiang Cancer Hospital, Hangzhou 310022.
Hongguang
H
Zhao
Department of pathology, Zhejiang Cancer Hospital, Hangzhou 310022.
Linfeng
L
Zheng
Department of pharmacy, Zhejiang Chinese Medicine University.
Xinru
X
Li
1] Department of biochemistry, Institute of basic medical science, Zhejiang Medical College, Hangzhou 310053, China [2] Department of biochemistry, Institute of basic medical science, Zhejiang Medical College, Hangzhou 310053, China.
Lifang
L
Wang
D000483:Alleles; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D004938:Esophageal Neoplasms / Q000235:genetics*; D000077277:Esophageal Squamous Cell Carcinoma; D053222:Fas Ligand Protein / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012306:Risk; D012307:Risk Factors
C505661:FASLG protein, human; D053222:Fas Ligand Protein