A pair of new antioxidant phenolic acid stereoisomers isolated from danshen injection (lyophilized powder).
A pair of new phenolic acid stereoisomers, (R)-norsalvianolic acid L (1) and (S)-norsalvianolic acid L (2), was isolated from the Danshen Injection (lyophilized powder). The structural elucidation and stereochemistry determination were achieved by spectroscopic and chemical methods including 1D, 2D NMR (1H-1H COSY, HSQC and HMBC) and circular dichroism experiments. Their antioxidant activities were assessed by the DPPH· and ABTS·+ scavenging methods in vitro with microplate assay. The IC50 values of 1 were 6.9 and 9.7 μM respectively, which was close to the control salvianolic acid B (7.8 and 7.1 μM respectively), while the IC50 values of isomer 2 were 27.1 and 25.3 μM, respectively.
National Institutes for Food and Drug Control, Beijing 100050, China.
Jing
J
Liu
National Institutes for Food and Drug Control, Beijing 100050, China.
Jianfeng
J
Zhao
National Institutes for Food and Drug Control, Beijing 100050, China.
Zhong
Z
Dai
National Institutes for Food and Drug Control, Beijing 100050, China. linrch307@sina.com.
Ruichao
R
Lin
National Institutes for Food and Drug Control, Beijing 100050, China. duneer@163.com.
Gangli
G
Wang
National Institutes for Food and Drug Control, Beijing 100050, China.
Shuangcheng
S
Ma
D000475:Alkenes / Q000737:chemistry; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry; D062385:Hydroxybenzoates / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry; D059808:Polyphenols / Q000737:chemistry; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D013237:Stereoisomerism
D000475:Alkenes; D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D062385:Hydroxybenzoates; D010636:Phenols; D059808:Polyphenols; C568740:salvianolic acid; C028972:dan-shen root extract; C017616:phenolic acid