UHPLC/Q-TOF MS-based plasma metabolic profiling analysis of the bleeding mechanism in a rat model of yeast and ethanol-induced blood heat and hemorrhage syndrome.
Blood heat and hemorrhage (BHH) syndrome is the most common bleeding disease in clinic. In this study, a rat model with BHH syndrome was built for the first time. Biochemical study showed the intrinsic coagulation pathways and the platelet aggregation rate in the rat model were inhibited, while extrinsic pathway of coagulation cascade was activated. An UHPLC/Q-TOF MS combined with orthogonal partial least squares-discriminant analysis (OPLS-DA) was employed to construct plasma metabolic profiling of the rat model with BHH syndrome. Twenty-four unique metabolites were identified, which were involved in glycerophospholipid metabolism, arachidonic acid metabolism, fatty acid metabolism, amino acid metabolism and cholic acid metabolism. In the end, we concluded that bleeding mechanism of the rat with BHH syndrome may be associated with augmenting blood viscosity, inhibiting platelet aggregation and intrinsic coagulation pathways.
Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, 138 Xianlin Road, Nanjing 210046, China.
Jing
J
Shang
Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, 138 Xianlin Road, Nanjing 210046, China.
Jia
J
Liu
Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, 138 Xianlin Road, Nanjing 210046, China.
Mu
M
He
Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, 138 Xianlin Road, Nanjing 210046, China.
Erxin
E
Shang
Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, 138 Xianlin Road, Nanjing 210046, China.
Li
L
Zhang
Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, 138 Xianlin Road, Nanjing 210046, China.
Mingqiu
M
Shan
Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, 138 Xianlin Road, Nanjing 210046, China.
Weifeng
W
Yao
Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, 138 Xianlin Road, Nanjing 210046, China.
Bing
B
Yu
Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, 138 Xianlin Road, Nanjing 210046, China.
Yingzhi
Y
Yao
Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, 138 Xianlin Road, Nanjing 210046, China. Electronic address: awding105@163.com.
Anwei
A
Ding
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D001777:Blood Coagulation / Q000502:physiology; D019826:Cholic Acid / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D016002:Discriminant Analysis; D000431:Ethanol / Q000009:adverse effects; D005227:Fatty Acids / Q000378:metabolism; D020404:Glycerophospholipids / Q000378:metabolism; D006470:Hemorrhage / Q000378:metabolism*; D006358:Hot Temperature; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D055442:Metabolome / Q000502:physiology*; D055432:Metabolomics / Q000379:methods; D023421:Models, Animal; D010974:Platelet Aggregation / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015003:Yeasts
D000596:Amino Acids; D005227:Fatty Acids; D020404:Glycerophospholipids; D016718:Arachidonic Acid; D000431:Ethanol; D019826:Cholic Acid
Blood heat and hemorrhage syndrome; Metabonomics; OPLS-DA; UHPLC/Q-TOF MS