Propagation of Vatica diospyroides symington: an endangered medicinal dipterocarp of peninsular Thailand by cultures of embryonic axes and leaf-derived calli.
Embryonic axes and young leaves of Vatica diospyroides Symington were excised sterilely and then cultured on MS medium supplemented with 0-20 mg L(-1) of a growth regulator and 0-0.3% Activated Charcoal (AC). The growth regulators tested were 2, 4-Dichlorophenoxyacetic acid (2,4-D), alpha-Naphthaleneacetic acid (NAA) and 3, 6-Dichloro-2-methoxybensoic acid (Dicamba). Vigorous shoot development was observed with 0-20 mg L(-1) of each plant growth regulator and 0.1-0.15% AC. Shoot-derived calli were obtained 6 months after culturing embryonic axes on MS medium, with 15 mg L(-1) dicamba and 0.1% AC. Interestingly, after culturing young leaves for 2 months, the highest weight of compact calli (0.40 g) was achieved with a medium containing 20 mg L(-1) dicamba and 0.1% AC, in darkness. These were successfully multiplied by renewing and culturing in the same medium and transfer to shooting induction in MS medium supplemented with 0-20 mg L(-1) 6-benzyladenine (BA). Unfortunately, shoot induction from calli was unsuccessful and despite initiated roots being induced. A successful in vitro propagation protocol of V. diospyroides should be thus investigated more extensively.
Theera Srisawat and Nathinan Jongkraijak Faculty of Science and Industrial Technology, Prince of Songkla University, Suratthani campus, Suratthani, 84000, Thailand.
Theera
T
Srisawat
Theera Srisawat and Nathinan Jongkraijak Faculty of Science and Industrial Technology, Prince of Songkla University, Suratthani campus, Suratthani, 84000, Thailand.
Nathinan
N
Jongkraijak
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D002606:Charcoal / Q000494:pharmacology; D003247:Conservation of Natural Resources; D003996:Dicamba / Q000494:pharmacology; D053958:Dipterocarpaceae / Q000145:classification / Q000187:drug effects / Q000196:embryology / Q000254:growth & development*; D056727:Endangered Species; D018525:Germination; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D008517:Phytotherapy; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves; D010946:Plants, Medicinal; D013785:Thailand; D013997:Time Factors
D009280:Naphthaleneacetic Acids; D010937:Plant Growth Regulators; D002606:Charcoal; D015084:2,4-Dichlorophenoxyacetic Acid; C034182:1-naphthaleneacetic acid; D003996:Dicamba