Oxidative stress markers correlate with renal dysfunction and thrombocytopenia in severe leptospirosis.
Leptospirosis is a zoonotic disease that causes severe manifestations such as Weil's disease and pulmonary hemorrhage syndrome. The aim of this study was to evaluate whether reactive oxygen species (ROS) production and antioxidant reduced glutathione (GSH) levels are related to complications in patients hospitalized with leptospirosis. The ROS production and GSH levels were measured in blood samples of 12 patients and nine healthy controls using chemiluminescence and absorbance assays. We found that ROS production was higher and GSH levels were lower in leptospirosis patients compared with healthy individuals. Among patients, GSH depletion was correlated with thrombocytopenia and elevated serum creatinine, whereas a strong positive correlation was observed between ROS production and elevated serum potassium. Additional investigation of the biological significance of ROS production and GSH levels is warranted as they may guide the development of novel adjuvant therapies for leptospirosis targeting oxidative stress.
Laboratory of Pathology and Molecular Biology, Oswaldo Cruz Foundation, Salvador, Bahia, Brazil; Faculty of Medicine, Federal University of Bahia, Salvador, BA, Brazil; Institute of Collective Health, Federal University of Bahia, Salvador, BA, Brazil; Department of Epidemiology of Microbial Diseases, Yale School of Public Health New Haven, Connecticut.
Alan M
AM
Araújo
Eliana A G
EA
Reis
Daniel A
DA
Athanazio
Guilherme S
GS
Ribeiro
José E
JE
Hagan
Guilherme C
GC
Araujo
Alcineia O
AO
Damião
Nicolli S
NS
Couto
Albert I
AI
Ko
Alberto
A
Noronha-Dutra
Mitermayer G
MG
Reis
D058186:Acute Kidney Injury / Q000097:blood*; D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D015331:Cohort Studies; D003404:Creatinine / Q000097:blood; D005260:Female; D005978:Glutathione / Q000097:blood*; D006801:Humans; D007922:Leptospirosis / Q000097:blood*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D011188:Potassium / Q000097:blood; D017382:Reactive Oxygen Species / Q000097:blood*; D012720:Severity of Illness Index; D013921:Thrombocytopenia / Q000097:blood*; D055815:Young Adult
D015415:Biomarkers; D017382:Reactive Oxygen Species; D003404:Creatinine; D005978:Glutathione; D011188:Potassium