Biotype expression and insecticide response of Bemisia tabaci chemosensory protein-1.
Chemosensory proteins (CSPs) are a group of small soluble proteins found so far exclusively in arthropod species. These proteins act in chemical communication and perception. In this study, a gene encoding the Type 1 CSP (BtabCSP1) from the agricultural pest Bemisia tabaci (whitefly) was analyzed to understand sequence variation and expression specificity in different biotypes. Sequence analysis of BtabCSP1 showed significant differences between the two genetically characterized biotypes, B and Q. The B-biotype had a larger number of BtabCSP1 mutations than the Q-biotype. Similar to most other CSPs, BtabCSP1 was more expressed in the head than in the rest of the body. One-step RT-PCR and qPCR analysis on total messenger RNA showed that biotype-Q had higher BtabCSP1 expression levels than biotype-B. Females from a mixed field-population had high levels of BtabCSP1 expression. The interaction of BtabCSP1 with the insecticide thiamethoxam was investigated by analyzing the BtabCSP1 expression levels following exposure to the neonicotinoid, thiamethoxam, in a time/dose-response study. Insecticide exposure increased BtabCSP1 expression (up to tenfold) at 4 and 24 h following 50 or 100 g/ml treatments.
Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Shandong Provincial Key Laboratory for Genetic Improvement Cultivation and Physiology of Crops, Jinan, China.
Guo Xia
GX
Liu
Ning
N
Xuan
Dong
D
Chu
Hong Yan
HY
Xie
Zhong Xue
ZX
Fan
Yu Ping
YP
Bi
Jean-François
JF
Picimbon
Yu Chuan
YC
Qin
Su Ting
ST
Zhong
Yao Fa
YF
Li
Zhan Lin
ZL
Gao
Wen Liang
WL
Pan
Guo Ying
GY
Wang
Balaji
B
Rajashekar
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006430:Hemiptera / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007306:Insecticides / Q000494:pharmacology*; D008297:Male; D008969:Molecular Sequence Data; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000494:pharmacology*; D009928:Organ Specificity; D010078:Oxazines / Q000494:pharmacology*; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D012727:Sex Characteristics; D000077922:Thiamethoxam; D013844:Thiazoles / Q000494:pharmacology*
D019476:Insect Proteins; D007306:Insecticides; D000073943:Neonicotinoids; D009574:Nitro Compounds; D010078:Oxazines; D013844:Thiazoles; D000077922:Thiamethoxam
B-biotype; Q-biotype; chemosensory protein; neonicotinoid thiamethoxam insecticide; whitefly