[Research on effect of Baimai powder effective compounds group promotes neurogenesis and maintains of neural stem cells after cerebral infarction].
The neural stem cells (NSCs), play a crucial role in stroke treatment, which can be regulated by a few of traditional Chinese medicines. In this study, the effect of the Mongolian medicine Baimai powder effective compounds group (BMECG) on the proliferation of NSCs has been investigated. The cultured NSCs which were isolated from newborn rat cerebral cortical in vitro were exposed to oxygen glucose deprivation/reoxgenation (OGD/R). The CFSE immunofluorescence staining was employed to identify the proliferation of NSCs by flow cytometry. Furthermore, the bilateral carotid artery occlusion (BCAO) was established on Kunming mice, and all groups were ig for 7 d respectively. The neurobehavioral changes was studied with rota-rod treadmill test, after that, the brain of mice were detected by immunohistochemistry with labeling of Nestin and pathological observation at 7 days after BCAO. It was found that, proliferation of NSCs was increased by BMECG in in vitro and in vivo. And BMECG significantly improved the time of staying in the rota-rod, it can promote the foundction of in cerebral cortex. It is concluded that these results further support the hypothesis that neuroprotective effect of BMECG may relate to the ability of stimulating self-renew of NSCs, which can be provided a new insight and strategy of anti-neuropathy of stroke.
Minzu University of China, State Nationalities Affairs Commission and Department of Educational Key Lab of Minority Traditional Medicine, Beijing 100081, China. nlqsh@163.com
Qing-Shan
QS
Liu
Minzu University of China, State Nationalities Affairs Commission and Department of Educational Key Lab of Minority Traditional Medicine, Beijing 100081, China.
Xiao-Yu
XY
Chen
Minzu University of China, State Nationalities Affairs Commission and Department of Educational Key Lab of Minority Traditional Medicine, Beijing 100081, China.
Shu-Juan
SJ
Zhuang
Minzu University of China, State Nationalities Affairs Commission and Department of Educational Key Lab of Minority Traditional Medicine, Beijing 100081, China.
Ke-Qin
KQ
Li
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002544:Cerebral Infarction / Q000188:drug therapy* / Q000503:physiopathology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D008297:Male; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology* / Q000187:drug effects; D055495:Neurogenesis / Q000187:drug effects*; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents