Development of a novel multi-layer microfluidic device towards characterization of drug metabolism and cytotoxicity for drug screening.
A novel multi-layer microfluidic device was developed for characterization of drug metabolism in human liver microsomes (HLMs) and their cytotoxicity on cells. The results demonstrated that this platform is robust for low levels of compounds and shows potential for high-throughput drug screening in drug development.
Department of Chemistry, Tsinghua University, Beijing, 100084, China.
Qin
Q
Wu
Dan
D
Gao
Juntong
J
Wei
Feng
F
Jin
Weiyi
W
Xie
Yuyang
Y
Jiang
Hongxia
H
Liu
D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D018844:Cyclin-Dependent Kinases / Q000037:antagonists & inhibitors; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D005419:Flavonoids / Q000494:pharmacology; D057166:High-Throughput Screening Assays / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D046210:Microfluidic Analytical Techniques; D008862:Microsomes, Liver / Q000378:metabolism; D010880:Piperidines / Q000494:pharmacology; D047428:Protein Kinase Inhibitors / Q000494:pharmacology
D005419:Flavonoids; D010880:Piperidines; D047428:Protein Kinase Inhibitors; C077990:alvocidib; D018844:Cyclin-Dependent Kinases