Proteome-driven elucidation of adaptive responses to combined vitamin E and C deficiency in zebrafish.
The purpose of this study was to determine the system-wide consequences of deficiencies in two essential micronutrients, vitamins E and C, on the proteome using zebrafish (Danio rerio) as one of the few vertebrate models that similar to humans cannot synthesize vitamin C. We describe a label-free proteomics workflow to detect changes in protein abundance estimates dependent on vitamin regimes. We used ion-mobility-enhanced data-independent tandem mass spectrometry to determine differential regulation of proteins in response to low dietary levels of vitamin C with or without vitamin E. The detection limit of the method was as low as 20 amol, and the dynamic range was five orders of magnitude for the protein-level estimates. On the basis of the quantitative changes obtained, we built a network of protein interactions that reflect the whole organism's response to vitamin C deficiency. The proteomics-driven study revealed that in vitamin-E-deficient fish, vitamin C deficiency is associated with induction of stress response, astrogliosis, and a shift from glycolysis to glutaminolysis as an alternative mechanism to satisfy cellular energy requirements.
Department of Chemistry, Oregon State University , 153 Gilbert Hall, Corvallis, Oregon 97331, United States.
Ievgen
I
Motorykin
Maret G
MG
Traber
Robert L
RL
Tanguay
Claudia S
CS
Maier
D000222:Adaptation, Physiological; D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000378:metabolism*; D001206:Ascorbic Acid Deficiency / Q000378:metabolism*; D002853:Chromatography, Liquid; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D053858:Metabolic Networks and Pathways; D025941:Protein Interaction Mapping; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D014020:Tissue Extracts / Q000737:chemistry; D014810:Vitamin E / Q000008:administration & dosage / Q000378:metabolism*; D014811:Vitamin E Deficiency / Q000378:metabolism*; D015027:Zebrafish
D020543:Proteome; D014020:Tissue Extracts; D014810:Vitamin E; D001205:Ascorbic Acid