Optimization of pressurized liquid extraction of inositols from pine nuts (Pinus pinea L.).
Pressurized liquid extraction (PLE) has been used for the first time to extract bioactive inositols from pine nuts. The influence of extraction time, temperature and cycles of extraction in the yield and composition of the extract was studied. A quadratic lineal model using multiple linear regression in the stepwise mode was used to evaluate possible trends in the process. Under optimised PLE conditions (50°C, 18 min, 3 cycles of 1.5 mL water each one) at 10 MPa, a noticeable reduction in extraction time and solvent volume, compared with solid-liquid extraction (SLE; room temperature, 2h, 2 cycles of 5 mL water each one) was achieved; 5.7 mg/g inositols were extracted by PLE, whereas yields of only 3.7 mg/g were obtained by SLE. Subsequent incubation of PLE extracts with Saccharomyces cerevisiae (37°C, 5h) allowed the removal of other co-extracted low molecular weight carbohydrates which may interfere in the bioactivity of inositols.
Instituto de Química Orgánica General (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain.
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Ruiz-Aceituno
Instituto de Química Orgánica General (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain.
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Rodríguez-Sánchez
Instituto de Química Orgánica General (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain.
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Sanz
Instituto de Química Orgánica General (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain. Electronic address: mlsanz@iqog.csic.es.
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Sanz
Instituto de Química Orgánica General (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain.
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Ramos
D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D007294:Inositol / Q000302:isolation & purification*; D009754:Nuts / Q000737:chemistry*; D028223:Pinus / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification*; D011312:Pressure
D010936:Plant Extracts; D007294:Inositol
Glycosyl-inositols; Inositols; Pine nuts; Pressurized liquid extraction; Saccharomyces cerevisiae