Nonlinear light-sheet fluorescence microscopy by photobleaching imprinting.
We present a nonlinear light-sheet fluorescence microscopy (LSFM) scheme based on photobleaching imprinting. By measuring photobleaching-induced fluorescence decay, our method simultaneously achieves a large imaging field of view and a thin optical section. Furthermore, the scattered-light-induced background is significantly reduced, considerably improving image contrast. Our method is expected to expand the application field of LSFM into the optical quasi-ballistic regime, enabling studies on non-transparent biological samples.
Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University, , St Louis, MO 63130, USA.
Liang
L
Gao
Liren
L
Zhu
Chiye
C
Li
Lihong V
LV
Wang
D000818:Animals; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D008962:Models, Theoretical; D038761:Photobleaching; D015027:Zebrafish / Q000196:embryology
light-sheet fluorescence microscopy; nonlinear microscopy; photobleaching