Comparison of supplementation of n-3 fatty acids from fish and flax oil on cytokine gene expression and growth of milk-fed Holstein calves.
The ability to reduce incidence of disease in calves and improve early vaccination strategies is of particular interest for dairy producers. The n-3 fatty acids have been reported to reduce inflammatory diseases in humans but limited research has been done in calves. The objective of this study was to compare supplementation of n-3 fatty acids from fish and flax oil on gene expression of whole blood cells and growth of milk-fed Holstein calves. Forty-eight Holstein bull calves from a commercial dairy were randomly assigned to 1 of 3 diets beginning at 4d old: (1) control milk replacer (MR) with all pork fat, (2) MR with 2% flax oil, and (3) MR with 2% fish oil. All MR were 17% fat, 27% crude protein on a dry matter (DM) basis, with all protein from whey sources. Calves were each fed 654g DM of MR daily for the first 25d and then 327g/d for d26, 27, and 28. On d28, calves were challenged with a Pasteurella vaccine and the temperature response to the vaccine was recorded. Milk and feed intake and fecal scores were recorded daily, and body weight and hip width were recorded weekly. Blood was collected from all calves on d25. One tube of collected blood was incubated with endotoxin (lipopolysaccharide; LPS) for 2h and frozen with a second tube of control blood. Quantitative real-time PCR was used to assess the effects of LPS stimulation on cytokine gene expression. During the 28 d, calves supplemented with flax oil had a greater growth rate and feed efficiency than calves fed fish oil (0.52±0.02 vs. 0.48±0.02g of gain:g of feed). Fish oil tended to decrease LPS stimulation of tumor necrosis factor-α expression. Flax oil, but not fish oil, decreased the expression of IL-4 and tended to decrease expression of osteopontin and IL-8. Flax oil tended to reduce the increase in rectal temperature in response to a Pasteurella vaccine. In conclusion, our data support the idea that supplementation with n-3 fatty acids affects cytokine gene expression.
Department of Animal Science, Michigan State University, East Lansing 48824.
E L
EL
Karcher
Nurture Research Center, Provimi North America, Brookville, OH 45309.
T M
TM
Hill
Nurture Research Center, Provimi North America, Brookville, OH 45309.
H G
HG
Bateman
Nurture Research Center, Provimi North America, Brookville, OH 45309.
R L
RL
Schlotterbeck
Department of Animal Science, Michigan State University, East Lansing 48824.
N
N
Vito
Department of Large Animal Clinical Sciences, Michigan State University, East Lansing 48824.
L M
LM
Sordillo
Department of Animal Science, Michigan State University, East Lansing 48824. Electronic address: mikevh@msu.edu.
M J
MJ
Vandehaar
D000821:Animal Feed; D000818:Animals; D001835:Body Weight; D002417:Cattle; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage*; D005395:Fish Oils / Q000008:administration & dosage*; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D008043:Linseed Oil / Q000008:administration & dosage*; D008070:Lipopolysaccharides / Q000009:adverse effects; D060888:Real-Time Polymerase Chain Reaction; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D016209:Interleukin-8; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D008043:Linseed Oil
calves; inflammation; n-3 fatty acids