Reentrant liquid-liquid phase separation in protein solutions at elevated hydrostatic pressures.
We present results from small-angle x-ray scattering data on the effect of high pressure on the phase behavior of dense lysozyme solutions in the liquid-liquid phase separation region, and characterize the underlying intermolecular protein-protein interactions as a function of temperature and pressure in this region of phase space. A reentrant liquid-liquid phase separation region has been discovered at elevated pressures, which originates in the pressure dependence of the solvent-mediated protein-protein interactions.
Fakultät Physik/DELTA, TU Dortmund, 44221 Dortmund, Germany.
Johannes
J
Möller
Physikalische Chemie, Fakultät für Chemie und Chemische Biologie, TU Dortmund, Otto-Hahn Strasse 6, 44227 Dortmund, Germany.
Sebastian
S
Grobelny
Fakultät Physik/DELTA, TU Dortmund, 44221 Dortmund, Germany.
Julian
J
Schulze
Fakultät Physik/DELTA, TU Dortmund, 44221 Dortmund, Germany.
Steffen
S
Bieder
Fakultät Physik/DELTA, TU Dortmund, 44221 Dortmund, Germany.
Andre
A
Steffen
Physikalische Chemie, Fakultät für Chemie und Chemische Biologie, TU Dortmund, Otto-Hahn Strasse 6, 44227 Dortmund, Germany.
Mirko
M
Erlkamp
Fakultät Physik/DELTA, TU Dortmund, 44221 Dortmund, Germany.
Michael
M
Paulus
Fakultät Physik/DELTA, TU Dortmund, 44221 Dortmund, Germany.
Metin
M
Tolan
Physikalische Chemie, Fakultät für Chemie und Chemische Biologie, TU Dortmund, Otto-Hahn Strasse 6, 44227 Dortmund, Germany.
Roland
R
Winter
D003102:Colloids / Q000737:chemistry; D006874:Hydrostatic Pressure; D008956:Models, Chemical; D009113:Muramidase / Q000737:chemistry*; D044367:Phase Transition; D012996:Solutions / Q000737:chemistry
D003102:Colloids; D012996:Solutions; D009113:Muramidase