[Sequence analysis and identification of a chloroplast matK gene in Rhei Rhizoma from different botanical origins].
Rhei Rhizoma is a Chinese medicine with multiple botanical origins. There is a problem to identify it with conventional methods. To compare the characteristics of chloroplast matK gene sequences of different Rheum species and authenticate inspected species, the matK gene sequences of different species from different origins were amplified, cloned, and sequenced. Genomic DNA of Rheum plants was extracted using modified DNA extracted Kit and matK gene sequences were analyzed by ContingExpress, DNAman and MEGA5.0. The length of matK gene sequences of Rheum palmatum, R. tanguticum and R. officinale were 1 518 bp containing 57 variable loci. According to the mutation sites, R. palmatum, R. tanguticum and R. officinale were divided into different genotypes separately. Based on the established method according to the loci 587, 707, 838, we successfully identified the genuine Rheum species from its adulterants.
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
Xiao-qin
XQ
Zhang
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
Chun-sheng
CS
Liu
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
Xing-li
XL
Yan
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
Xiao-li
XL
Cheng
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
Juan
J
Liu
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
Qiu-ling
QL
Wang
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
Kai
K
Liu
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
Sheng-li
SL
Wei
D000595:Amino Acid Sequence; D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics; D004340:Drug Contamination; D061125:Genes, Chloroplast; D017343:Genes, Plant; D005838:Genotype; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D010946:Plants, Medicinal / Q000235:genetics*; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000235:genetics*; D012250:Rheum / Q000145:classification / Q000235:genetics*; D027343:Rhizome / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D018744:DNA, Plant; C085377:MATK protein, human; D016392:Proto-Oncogene Proteins pp60(c-src)