Confocal supercritical angle microscopy for cell membrane imaging.
We demonstrate subwavelength sectioning on biological samples with a conventional confocal microscope. This optical sectioning is achieved by the phenomenon of supercritical angle fluorescence, wherein only a fluorophore next to the interface of a refractive index discontinuity can emit propagating components of radiation into the so-called forbidden angles. The simplicity of this technique allows it to be integrated with a high numerical aperture confocal scanning microscope by only a simple modification on the detection channel. Confocal-supercritical angular fluorescence microscopy would be a powerful tool to achieve high-resolution surface imaging, especially for membrane imaging in biological samples.
Siddharth
S
Sivankutty
Thomas
T
Barroca
Céline
C
Mayet
Guillaume
G
Dupuis
Emmanuel
E
Fort
Sandrine
S
Lévêque-Fort
D000818:Animals; D016466:CHO Cells; D002462:Cell Membrane / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D018613:Microscopy, Confocal / Q000379:methods*