Cloning and characterization of a dynein light chain gene from Puccinia striiformis f. sp. tritici.
Stripe rust is one of the most serious wheat diseases worldwide. The fungus Puccinia striiformis f. sp. tritici (Pst), the causal agent of this disease, is an obligate biotrophic basidiomycete fungus. Numerous studies have shown that dyneins play important roles during fungal growth and propagation. However, knowledge is limited regarding the function of dyneins in Pst. In this study, we cloned the dynein light chain gene PsDLC1 from Pst and characterized its expression. The function of PsDLC1 was determined by heterologous mutant complementation. Expression of PsDLC1 in Aspergillus nidulans partially complemented the defects of the ΔnudG mutant, indicating that PsDLC1 belongs to the dynein light chain LC8 family. In addition, PsDLC1 was identified in Pst using virus-induced gene silencing (VIGS). Knockdown of PsDLC1 produces no significant effect on Pst growth and development, indicating that PsDLC1 is unnecessary for Pst infection of wheat.
College of Life Sciences, State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling, Shaanxi, China.
Jie
J
Liu
Qiong
Q
Zhang
Qing
Q
Chang
Qiuling
Q
Wang
Lina
L
Han
Jia
J
Liu
Man
M
Li
Hua
H
Zhuang
Zhensheng
Z
Kang
D001233:Aspergillus nidulans / Q000235:genetics / Q000254:growth & development; D001487:Basidiomycota / Q000235:genetics* / Q000254:growth & development* / Q000302:isolation & purification; D003001:Cloning, Molecular; D004398:Dyneins / Q000235:genetics* / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D020869:Gene Expression Profiling; D055785:Gene Knockdown Techniques; D005816:Genetic Complementation Test; D010935:Plant Diseases / Q000382:microbiology; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D014908:Triticum / Q000382:microbiology
D005656:Fungal Proteins; D021122:Protein Subunits; D004398:Dyneins
Dynein; Light chain 8; Puccinia striiformis f. sp. tritici; Stripe rust