Failure to find evidence for deletion of LCE3C and LCE3B genes at PSORS4 contributing to psoriasis susceptibility in Tunisian families.
BACKGROUND
Recently, it has been shown that a deletion in the late cornified envelope (LCE) gene cluster (LCE3C_LCE3B-del) is associated with susceptibility to psoriasis in European and Asian populations. However, no study of this deletion has been performed in the North African population. The aim of the present study was to investigate whether this deletion is associated with familial psoriasis in Tunisian population.


METHODS
A total of 34 patients and 55 healthy individuals were recruited from 7 multiplex families and a PCR assay was used to determine the association of this deletion. Its effect on susceptibility to psoriasis was assessed using the PDT program.


RESULTS
We failed to detect any evidence of association between LCE3C_LCE3B-del and psoriasis in Tunisian families. No epistasic effect was found between the deletion and PSORS1 locus.


CONCLUSIONS
These findings indicate that the LCE3C_LCE3B-del does not contribute in a major way to psoriasis susceptibility in Tunisian families.
Laboratory of Genetics, Immunology and Human Pathologies, Department of Biology, Faculty of Sciences, Campus Universitaire, El Manar, 2092 Tunis, Tunisia. Electronic address: myriambensaid@yahoo.fr.
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M
Ammar
Laboratory of Genetics, Immunology and Human Pathologies, Department of Biology, Faculty of Sciences, Campus Universitaire, El Manar, 2092 Tunis, Tunisia.
C
C
Bouchlaka-Souissi
Laboratory of Genetics, Immunology and Human Pathologies, Department of Biology, Faculty of Sciences, Campus Universitaire, El Manar, 2092 Tunis, Tunisia.
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K
Soumaya
Laboratory of Genetics, Immunology and Human Pathologies, Department of Biology, Faculty of Sciences, Campus Universitaire, El Manar, 2092 Tunis, Tunisia.
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R
Bouhaha
Department of Dermatology, La Rabta Hospital, La Rabta, 1007 Tunis, Tunisia.
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Z
Ines
Laboratory of Genetics, Immunology and Human Pathologies, Department of Biology, Faculty of Sciences, Campus Universitaire, El Manar, 2092 Tunis, Tunisia.
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F
Bouazizi
Department of Dermatology, Military Hospital of Tunis, Montfleury, Tunis, Tunisia.
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N
Doss
Department of Dermatology, Military Hospital of Tunis, Montfleury, Tunis, Tunisia.
R
R
Dhaoui
Department of Dermatology, La Rabta Hospital, La Rabta, 1007 Tunis, Tunisia.
A
A
Ben Osman
Laboratory of Genetics, Immunology and Human Pathologies, Department of Biology, Faculty of Sciences, Campus Universitaire, El Manar, 2092 Tunis, Tunisia.
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A
Ben Ammar-El Gaaïed
Department of Dermatology, La Rabta Hospital, La Rabta, 1007 Tunis, Tunisia.
M
M
Mokni
Laboratory of Genetics, Immunology and Human Pathologies, Department of Biology, Faculty of Sciences, Campus Universitaire, El Manar, 2092 Tunis, Tunisia.
R
R
Marrakchi
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics; D055233:Cornified Envelope Proline-Rich Proteins / Q000172:deficiency* / Q000235:genetics; D004843:Epistasis, Genetic; D005192:Family Health; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D015236:HLA-C Antigens / Q000235:genetics; D006801:Humans; D054643:INDEL Mutation; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011565:Psoriasis / Q000453:epidemiology / Q000208:ethnology / Q000235:genetics*; D017384:Sequence Deletion; D014416:Tunisia / Q000453:epidemiology / Q000208:ethnology; D055815:Young Adult
D055233:Cornified Envelope Proline-Rich Proteins; D015236:HLA-C Antigens; C528994:HLA-C*06 antigen; C549748:LCE3B protein, human; C524768:LCE3C protein, human
Copy number variation; Family-based association study; Gene interaction; Genetics; Génétique; Interaction gène-gène; Psoriasis; Variation de nombre de copies; Étude d’association basée sur des familles