[Expression of triggering receptors expressed on myeloid-1 in innate response to Porphyromonas gingivalis in macrophages].
OBJECTIVE
To investigate the role of triggering receptors expressed on myeloid-1(TREM-1) in innate response to Porphyromonas gingivalis(Pg) in mice macrophages and its potential role in periodontitis development.


METHODS
Peritoneal macrophages from mice were harvested, separated and cultured, then challenged with viable Pg. Transcription and protein expression in macrophages were assessed with real time PCR and flow cytometry respectively.LP-17 peptide (10, 100 and 1000 µg/L) was utilized to block TREM-1, and tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were detected by enzyme linked absorbent analysis.


RESULTS
At 2 h after Pg challenge, transcription of TREM-1 was significantly up-regulated after Pg challenge[(7.99 ± 1.11) fold vs blank]. At 24 h after bacteria infection, increased TREM-1 expression was demonstrated by flow cytometry, with mean fluorescent intensity increasing from (7.05 ± 1.85) in blank group to (13.17 ± 2.33) in experimental group. Proinflammatory cytokine (TNF-α and IL-6) production was significantly decreased after blocking TREM-1 by LP-17 peptide(100 and 1000 µg/L).


CONCLUSIONS
TREM-1 enhanced innate immune response to Pg in macrophages, which may facilitate periodontitis development.
Affiliated Stomatological Hospital, Fujian Medical University & Stomatological Key Laboratory of Fujian College and University, Fuzhou 350002, China.
Lang
L
Lei
Hou-xuan
HX
Li
Sheng-bo
SB
Pan
Department of Periodontology, School of Stomatology,Nanjing University & Nanjing Stomatological Hospital, Nanjing 210008, China,Email: fhyan2005@126.com.
Fu-hua
FH
Yan
D000818:Animals; D002478:Cells, Cultured; D015850:Interleukin-6 / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D010455:Peptides / Q000494:pharmacology; D016966:Porphyromonas gingivalis / Q000276:immunology; D011971:Receptors, Immunologic / Q000378:metabolism*; D000073871:Triggering Receptor Expressed on Myeloid Cells-1; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015854:Up-Regulation
D015850:Interleukin-6; D008562:Membrane Glycoproteins; D010455:Peptides; D011971:Receptors, Immunologic; C517262:TREM1 protein, mouse; D000073871:Triggering Receptor Expressed on Myeloid Cells-1; D014409:Tumor Necrosis Factor-alpha