Vernonia condensata Baker (Asteraceae): a promising source of antioxidants.
The present study evaluated the antioxidant potential of Vernonia condensata Baker (Asteraceae). Dried and powdered leaves were exhaustively extracted with ethanol by static maceration followed by partition to obtain the hexane, dichloromethane, ethyl acetate, and butanol fractions. Total phenols and flavonoids contents were determined through spectrophotometry and flavonoids were identified by HPLC-DAD system. The antioxidant activity was assessed by DPPH radical scavenging activity, TLC-bioautography, reducing power of Fe(+3), phosphomolybdenum, and TBA assays. The total phenolic content and total flavonoids ranged from 0.19 to 23.11 g/100 g and from 0.13 to 4.10 g/100 g, respectively. The flavonoids apigenin and luteolin were identified in the ethyl acetate fraction. The IC50 of DPPH assay varied from 4.28 to 75.10 µg/mL and TLC-bioautography detected the antioxidant compounds. The reducing power of Fe(+3) was 19.98 to 336.48  μg/mL, while the reaction with phosphomolybdenum ranged from 13.54% to 32.63% and 56.02% to 135.00% considering ascorbic acid and rutin as reference, respectively. At 30 mg/mL, the ethanolic extract and fractions revealed significant effect against lipid peroxidation. All these data sustain that V. condensata is an important and promising source of bioactive substances with antioxidant activity.
Graduate Program in Pharmaceutical Sciences, Federal University of Juiz de Fora, Campus Universitário, 36036-330 Juiz de Fora, MG, Brazil ; Faculty of Pharmacy, Federal University of Juiz de Fora, Campus Universitário, 36036-330 Juiz de Fora, MG, Brazil.
Jucélia Barbosa
JB
da Silva
Graduate Program in Pharmaceutical Sciences, Federal University of Juiz de Fora, Campus Universitário, 36036-330 Juiz de Fora, MG, Brazil ; Faculty of Pharmacy, Federal University of Juiz de Fora, Campus Universitário, 36036-330 Juiz de Fora, MG, Brazil.
Vanessa dos Santos
Vdos S
Temponi
Graduate Program in Pharmaceutical Sciences, Federal University of Juiz de Fora, Campus Universitário, 36036-330 Juiz de Fora, MG, Brazil ; Faculty of Pharmacy, Federal University of Juiz de Fora, Campus Universitário, 36036-330 Juiz de Fora, MG, Brazil.
Carolina Miranda
CM
Gasparetto
Faculty of Pharmacy, Federal University of Juiz de Fora, Campus Universitário, 36036-330 Juiz de Fora, MG, Brazil.
Rodrigo Luiz
RL
Fabri
Department of Biochemistry, Institute of Biological Sciences, Federal University of Juiz de Fora, Campus Universitário, 36036-330 Juiz de Fora, MG, Brazil.
Danielle Maria de Oliveira
DM
Aragão
Graduate Program in Pharmaceutical Sciences, Federal University of Juiz de Fora, Campus Universitário, 36036-330 Juiz de Fora, MG, Brazil.
Nícolas de Castro Campos
Nde C
Pinto
Department of Biochemistry, Institute of Biological Sciences, Federal University of Juiz de Fora, Campus Universitário, 36036-330 Juiz de Fora, MG, Brazil.
Antônia
A
Ribeiro
Graduate Program in Pharmaceutical Sciences, Federal University of Juiz de Fora, Campus Universitário, 36036-330 Juiz de Fora, MG, Brazil ; Department of Biochemistry, Institute of Biological Sciences, Federal University of Juiz de Fora, Campus Universitário, 36036-330 Juiz de Fora, MG, Brazil.
Elita
E
Scio
Faculty of Pharmacy, Federal University of Juiz de Fora, Campus Universitário, 36036-330 Juiz de Fora, MG, Brazil.
Glauciemar
G
Del-Vechio-Vieira
Graduate Program in Pharmaceutical Sciences, Federal University of Juiz de Fora, Campus Universitário, 36036-330 Juiz de Fora, MG, Brazil ; Faculty of Pharmacy, Federal University of Juiz de Fora, Campus Universitário, 36036-330 Juiz de Fora, MG, Brazil.
Orlando Vieira
OV
de Sousa
Graduate Program in Pharmaceutical Sciences, Federal University of Juiz de Fora, Campus Universitário, 36036-330 Juiz de Fora, MG, Brazil ; Faculty of Pharmacy, Federal University of Juiz de Fora, Campus Universitário, 36036-330 Juiz de Fora, MG, Brazil.
Maria Silvana
MS
Alves
D000085:Acetates / Q000737:chemistry; D000975:Antioxidants / Q000032:analysis*; D047310:Apigenin / Q000032:analysis; D001713:Biphenyl Compounds / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis; D016166:Free Radical Scavengers / Q000737:chemistry; D047311:Luteolin / Q000032:analysis; D008315:Malondialdehyde / Q000378:metabolism; D008982:Molybdenum; D010084:Oxidation-Reduction; D010636:Phenols / Q000032:analysis; D010756:Phosphoric Acids; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D035741:Vernonia / Q000737:chemistry*
D000085:Acetates; D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D016166:Free Radical Scavengers; D010636:Phenols; D010756:Phosphoric Acids; D010851:Picrates; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; D008315:Malondialdehyde; C007650:ethyl acetate; D047310:Apigenin; D008982:Molybdenum; C004931:1,1-diphenyl-2-picrylhydrazyl; D047311:Luteolin; C003125:phosphomolybdic acid