A tuned affinity-based staurosporine probe for in situ profiling of protein kinases.
A clickable and cell-permeable affinity-based probe (AfBP) was designed from staurosporine, by incorporating an electrophilic chloroacetamide warhead to facilitate in situ proteome labeling of a range of potential kinase targets.
Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore117543. chmyaosq@nus.edu.sg.
Xiamin
X
Cheng
Lin
L
Li
Mahesh
M
Uttamchandani
Shao Q
SQ
Yao
D000081:Acetamides / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D062105:Molecular Docking Simulation; D047428:Protein Kinase Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D011494:Protein Kinases / Q000737:chemistry* / Q000378:metabolism; D040901:Proteomics; D019311:Staurosporine / Q000737:chemistry* / Q000494:pharmacology
D000081:Acetamides; D047428:Protein Kinase Inhibitors; C013874:chloroacetamide; D011494:Protein Kinases; D019311:Staurosporine