Dynamic photochemical lipid micropatterning for manipulation of nonadherent mammalian cells.
Cell micropatterning methods with stimuli-responsive dynamic surfaces are getting a lot of attention in a wide variety of research fields, ranging from cell engineering to fundamental studies in cell biology. The surface of a slide coated with photo-cleavable poly(ethylene glycol) (PEG)-lipid can be used to spatiotemporally control cell immobilization and release by light irradiation. On the basis of this surface, it is easy to design simple methods for making a fine micropattern of any kind of cell. Furthermore, target cells can be selectively and rapidly released from this surface by light irradiation. In this review, we first describe how to obtain the photo-cleavable PEG-lipid from commercially available compounds through a facile four-step synthesis. Next, as a cell-patterning method, the protocols of coating substrates with the PEG-lipid, irradiating a pattern of light onto the coated substrate, and loading cells onto the irradiated surface are described. These protocols require no expensive equipment and potentially apply to any substrates that can adsorb serum albumin or chemically expose amine moieties on their surfaces. Finally, as an advanced method, cell release from the PEG-lipid surface in microfluidic devices is introduced. We also discuss the advantages and the possible applications of the present dynamic cell-patterning method.
Department of Bioengineering, The University of Tokyo, Tokyo, Japan.
Shinya
S
Yamahira
Department of Bioengineering, The University of Tokyo, Tokyo, Japan.
Yumi
Y
Takasaki
Department of Chemistry and Biotechnology, The University of Tokyo, Tokyo, Japan.
Satoshi
S
Yamaguchi
Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan.
Kimio
K
Sumaru
Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan.
Toshiyuki
T
Kanamori
Department of Bioengineering, The University of Tokyo, Tokyo, Japan; Department of Chemistry and Biotechnology, The University of Tokyo, Tokyo, Japan.
Teruyuki
T
Nagamune
D000818:Animals; D002448:Cell Adhesion; D006801:Humans; D008027:Light; D008055:Lipids / Q000138:chemical synthesis / Q000737:chemistry*; D008322:Mammals / Q000378:metabolism*; D044085:Microfluidics; D055616:Microtechnology / Q000379:methods*; D055668:Photochemical Processes / Q000528:radiation effects; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry
D008055:Lipids; D011092:Polyethylene Glycols
Cell micropatterning; Microfluidics; Nonadherent cells; PEG–lipid; Photo-cleavable materials