Determination of 26 veterinary antibiotics residues in water matrices by lyophilization in combination with LC-MS/MS.
A sensitive, simple and reliable multi-residue method was developed for the determination of 26 widely used veterinary antibiotics including 6 macrolides, 2 pleuromutilins, 4 tetracyclines, 2 lincosamides, 6 fluoroquinolones and 6 sulfonamides in different water matrices using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Water samples were lyophilized to dryness. Target compounds were separated on Zorbax SB-Aq column (150mm×2.1mm i.d., 3.5μm) and determined by LC-MS/MS operating in positive electrospray ionization mode. Spiked at concentration levels of 0.02, 0.4 and 4μgL(-1), recoveries of all target compounds were over 70% except sulfaquinoxaline (59.0% at 0.02μgL(-1)) with relative standard deviations below 20%. Limits of detection (LOD) and limits of quantification (LOQ) of 26 drugs ranged from 0.1 to 6.5ngL(-1) and from 0.3 to 19ngL(-1), respectively. The developed method was successfully applied to the analysis of 26 antibiotics residues in fish pond water, groundwater, biogas digester water, and lagoon wastewater samples collected from local pig farms.
National Reference Laboratory of Veterinary Drug Residues (SCAU), College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Feng-Yang
FY
Hu
National Reference Laboratory of Veterinary Drug Residues (SCAU), College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Li-Min
LM
He
National Reference Laboratory of Veterinary Drug Residues (SCAU), College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Jian-Wen
JW
Yang
National Reference Laboratory of Veterinary Drug Residues (SCAU), College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Kui
K
Bian
National Reference Laboratory of Veterinary Drug Residues (SCAU), College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Zong-Nan
ZN
Wang
National Reference Laboratory of Veterinary Drug Residues (SCAU), College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Hai-Cui
HC
Yang
National Reference Laboratory of Veterinary Drug Residues (SCAU), College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. Electronic address: gale@scau.edu.cn.
Ya-Hong
YH
Liu
D000900:Anti-Bacterial Agents / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods; D004355:Drug Stability; D005612:Freeze Drying / Q000379:methods; D005618:Fresh Water / Q000737:chemistry*; D057230:Limit of Detection; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods; D019155:Veterinary Drugs / Q000032:analysis*; D062065:Wastewater / Q000737:chemistry*; D014873:Water Pollutants / Q000032:analysis*
D000900:Anti-Bacterial Agents; D019155:Veterinary Drugs; D062065:Wastewater; D014873:Water Pollutants
Antibiotics; Environmental water; Liquid chromatography–tandem mass spectrometry; Lyophilization