Biosynthesis of the insecticidal xenocyloins in Xenorhabdus bovienii.
The biosynthesis gene cluster for the production of xenocyloins was identified in the entomopathogenic bacterium Xenorhabdus bovienii SS-2004, and their biosynthesis was elucidated by heterologous expression and in vitro characterization of the enzymes. XclA is an S-selective ThDP-dependent acyloin-like condensation enzyme, and XclB and XclC are examples of the still-rare acylating ketosynthases that catalyze the acylation of the XclA-derived initial xenocyloins with acetyl-, propionyl-, or malonyl-CoA, thereby resulting in the formation of further xenocyloin derivatives. All xenocyloins were produced mainly by the more virulent primary variant of X. bovienii and showed activity against insect hemocytes thus contributing to the overall virulence of X. bovienii against insects.
Merck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Göthe Universität Frankfurt, Max-von-Laue-Strasse 9, 60438 Frankfurt am Main (Germany).
Anna
A
Proschak
Qiuqin
Q
Zhou
Tim
T
Schöner
Aunchalee
A
Thanwisai
Darko
D
Kresovic
Andrea
A
Dowling
Richard
R
ffrench-Constant
Ewgenij
E
Proschak
Helge B
HB
Bode
D015099:3-Oxoacyl-(Acyl-Carrier-Protein) Synthase / Q000235:genetics / Q000378:metabolism; D000215:Acylation; D000818:Animals; D001665:Binding Sites; D007211:Indoles / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D007306:Insecticides / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D007915:Lepidoptera / Q000187:drug effects; D062105:Molecular Docking Simulation; D005810:Multigene Family; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D020593:Xenorhabdus / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics
D007211:Indoles; D007306:Insecticides; D011994:Recombinant Proteins; D015099:3-Oxoacyl-(Acyl-Carrier-Protein) Synthase
S-hydroxyketones; Xenorhabdus; acylating ketosynthases; acyloin condensation; biosynthesis; insecticidal activity; natural products