Vitreoretinal lymphoma versus uveitis: cytokine profile and correlations.
OBJECTIVE
To compare the cytokine spectrum of vitreoretinal lymphoma to uveitis and correlate cytokine concentrations with disease activity.


METHODS
Retrospective case series of 10 patients with vitreoretinal lymphoma and 7 patients with uveitis. Aqueous humor concentration of IFN-γ, TNF-α, TNF-β, IL-1ra, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, IL-17, MCP-1, G-CSF, GM-CSF, and VEGF-A was determined using a bead-based assay (Luminex). Variance between groups, correlation coefficients, and longitudinal behavior of cytokines were analyzed.


RESULTS
No statistically significant difference in cytokines was found when comparing groups. IL-10 was positively correlated with IL-6 and MCP-1. IL-6 was positively correlated with G-CSF, IL-1ra, IL-8, and IL-10. A relationship between concentration of any cytokine, aside from IL-10, and disease activity was not found.


CONCLUSIONS
IL-10 and IL-6 are good immunologic markers to be used as complementary diagnostic tools. IL-10 is the only IL that could be used for monitoring purposes.
Department of Ophthalmology .
Juline N
JN
Caraballo
Melissa R
MR
Snyder
Patrick B
PB
Johnston
Brian P
BP
O Neill
Harish
H
Raja
Joseph G
JG
Balsanek
Brian E
BE
Peters
Jose S
JS
Pulido
D001082:Aqueous Humor / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism*; D005134:Eye Neoplasms / Q000175:diagnosis / Q000378:metabolism; D006801:Humans; D007118:Immunoassay; D016393:Lymphoma, B-Cell / Q000175:diagnosis* / Q000378:metabolism; D019572:Retinal Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D012189:Retrospective Studies; D014605:Uveitis / Q000175:diagnosis* / Q000378:metabolism; D014822:Vitreous Body / Q000473:pathology*
D015415:Biomarkers; D016207:Cytokines