Molecular analysis of an odorant-binding protein gene in two sympatric species of Lutzomyia longipalpis s.l.
Lutzomyia longipalpis s.l. is the main vector of American visceral leishmaniasis (AVL) and occurs as a species complex. DNA samples from two Brazilian sympatric species that differ in pheromone and courtship song production were used to analyse molecular polymorphisms in an odorant-binding protein (obp29) gene. OBPs are proteins related to olfaction and are involved in activities fundamental to survival, such as foraging, mating and choice of oviposition site. In this study, the marker obp29 was found to be highly polymorphic in Lu. longipalpis s.l. , with no fixed differences observed between the two species. A pairwise fixation index test indicated a moderate level of genetic differentiation between the samples analysed.
Laboratório de Biologia Molecular de Insetos, Instituto Oswaldo Cruz, Fiocruz, Brasil, Rio de JaneiroRJ, Laboratório de Biologia Molecular de Insetos, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brasil.
Ana Karina Kerche
AK
Dias
Laboratório de Biologia Molecular de Insetos, Instituto Oswaldo Cruz, Fiocruz, Brasil, Rio de JaneiroRJ, Laboratório de Biologia Molecular de Insetos, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brasil.
Luiz Guilherme Soares da Rocha
LG
Bauzer
Laboratório de Biologia Molecular de Insetos, Instituto Oswaldo Cruz, Fiocruz, Brasil, Rio de JaneiroRJ, Laboratório de Biologia Molecular de Insetos, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brasil.
Denise Borges dos
DB
Santos Dias
Laboratório de Biologia Molecular de Insetos, Instituto Oswaldo Cruz, Fiocruz, Brasil, Rio de JaneiroRJ, Laboratório de Biologia Molecular de Insetos, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brasil.
Alexandre Afranio
AA
Peixoto
D000818:Animals; D001938:Brazil; D056084:Genetic Fitness; D007303:Insect Vectors / Q000235:genetics*; D010675:Pheromones / Q000235:genetics; D011110:Polymorphism, Genetic / Q000235:genetics; D011576:Psychodidae / Q000235:genetics*; D018035:Receptors, Odorant / Q000235:genetics*; D060047:Reproductive Isolation; D017422:Sequence Analysis, DNA; D012726:Sexual Behavior, Animal / Q000502:physiology*; D061350:Sympatry / Q000235:genetics*
D010675:Pheromones; D018035:Receptors, Odorant; C045648:odorant-binding protein