Evaluation of the SD BIOLINE Dengue Duo rapid test in the course of acute and convalescent dengue infections in a Mexican endemic region.
In this study, we evaluated the performance of a rapid test, the SD BIOLINE Dengue Duo (SD BDD) kit, with a panel of serum samples from 310 Mexican patients with diagnosis of dengue infection previously confirmed by reference enzyme-linked immunosorbent assay tests. Eighty-seven negative samples from other febrile illnesses were included as controls. The SD BDD showed an overall sensitivity of 90.65% and specificity of 89.66%. No statistically significant differences were found in the sensitivity of the SD BDD kit compared between primary or secondary infections (87.05% versus 93.57%, respectively, P = 0.0761) and dengue fever or dengue hemorrhagic fever cases (90.77% versus 89.74%, respectively, P = 0.7716). However, a higher sensitivity in the acute phase of dengue infection was found compared with the convalescent phase (93.03% versus 81.82%, respectively, P = 0.0089). These results indicate that the SD BDD kit is a useful tool to diagnose dengue infections, both in primary or secondary infections and mainly during the acute phase.
Laboratorio Multidisciplinario en Ciencias Biomédicas, Instituto de Investigaciones Medico-Biológicas, Universidad Veracruzana. Avenida Iturbide S/N, Col. Centro, C.P, 91700 Veracruz, México.
Luis A
LA
Sánchez-Vargas
Laboratorio Multidisciplinario en Ciencias Biomédicas, Instituto de Investigaciones Medico-Biológicas, Universidad Veracruzana. Avenida Iturbide S/N, Col. Centro, C.P, 91700 Veracruz, México; Hospital Regional de Alta Especialidad de Veracruz, Servicios de Salud de Veracruz. Av. 20 de Noviembre, Col. Centro. C.P. 91700 Veracruz, México.
Elvis E
EE
Sánchez-Marce
Laboratorio Multidisciplinario en Ciencias Biomédicas, Instituto de Investigaciones Medico-Biológicas, Universidad Veracruzana. Avenida Iturbide S/N, Col. Centro, C.P, 91700 Veracruz, México. Electronic address: hvivanco@uv.mx.
Héctor
H
Vivanco-Cid
D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000097:blood; D003715:Dengue / Q000175:diagnosis*; D003955:Diagnostic Tests, Routine / Q000379:methods*; D006801:Humans; D007159:Immunologic Tests / Q000379:methods*; D008800:Mexico; D019095:Point-of-Care Systems; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral; D000956:Antigens, Viral
Dengue; Rapid test; SD Bioline Dengue Duo