Poly(methyl vinyl ether-alt-maleic acid)-functionalized porous silicon nanoparticles for enhanced stability and cellular internalization.
Currently, developing a stable nanocarrier with high cellular internalization and low toxicity is a key bottleneck in nanomedicine. Here, we have developed a successful method to covalently conjugate poly(methyl vinyl ether-co-maleic acid) (PMVE-MA) copolymer on the surface of (3-aminopropyl)triethoxysilane-functionalized thermally carbonized porous silicon nanoparticles (APSTCPSi NPs), forming a surface negatively charged nanovehicle with unique properties. This polymer conjugated NPs could modify surface smoothness, charge, and hydrophilicity of the developed NPs, leading to considerable improvement in the colloidal and plasma stabilities via enhanced suspensibility and charge repulsion. Furthermore, despite the surface negative charge of the polymer-conjugated NPs, the cellular internalization was increased in both MDA-MB-231 and MCF-7 breast cancer cells. These results provide a proof-of-concept evidence that such polymer-based PSi nanocomposite can be extensively used as a promising candidate for intracellular drug delivery.
Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, Fl-00014, Helsinki, Finland.
Mohammad-Ali
MA
Shahbazi
Patrick V
PV
Almeida
Ermei
E
Mäkilä
Alexandra
A
Correia
Mónica P A
MP
Ferreira
Martti
M
Kaasalainen
Jarno
J
Salonen
Jouni
J
Hirvonen
Hélder A
HA
Santos
D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002470:Cell Survival; D002477:Cells / Q000166:cytology*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D012825:Silicon / Q000737:chemistry*
D011108:Polymers; D012825:Silicon
cell uptake; nanoparticle; poly(methyl vinyl ether-alt-maleic acid); porous silicon; stability