Opiorphin increases blood pressure of conscious rats through renin-angiotensin system (RAS).
Human opiorphin is a recently identified endogenous pentapeptide, encoded by ProL1 multigenes family that contributes to cardiovascular modulation. The aim of this study was to evaluate the effect of opiorphin through intravenous injection (i.v.) on mean arterial pressure (MAP) regulation. To investigate the bioactivity of opiorphin, a rat cannulation model was developed for MAP measurement and blood sampling. In our present study, opiorphin (200-700 nmol/kg) increased MAP in dose-related and time-dependent manner in conscious rats, which associated highly with the elevation of angiotensin II (AngII) levels in serum. Furthermore, the MAP elevation induced by opiorphin was completely blocked by AngII receptor antagonist valsartan and partially attenuated by angiotensin-converting enzyme inhibitor captopril. Finally, we tested the effect of opiorphin in hypoxia condition, which exhibited that opiorphin reversed hypoxia induced hypotension in conscious rats. Taken together, these results indicated that opiorphin may play an important role in the modulation of blood pressure through AngII dependent pathway, which may help future development of potent clinical therapeutics for emergency treatment.
Department of Biochemistry and Molecular Biology, School of Life Sciences, Lanzhou University, 222 Tian Shui South Road, Lanzhou 730000, P.R. China.
Yuan
Y
Fang
Department of Biochemistry and Molecular Biology, School of Life Sciences, Lanzhou University, 222 Tian Shui South Road, Lanzhou 730000, P.R. China.
Shuo
S
Li
Department of Biochemistry and Molecular Biology, School of Life Sciences, Lanzhou University, 222 Tian Shui South Road, Lanzhou 730000, P.R. China.
Huabin
H
Zhou
Department of Biochemistry and Molecular Biology, School of Life Sciences, Lanzhou University, 222 Tian Shui South Road, Lanzhou 730000, P.R. China.
Xiaozhu
X
Tian
Department of Biochemistry and Molecular Biology, School of Life Sciences, Lanzhou University, 222 Tian Shui South Road, Lanzhou 730000, P.R. China.
Shuangyu
S
Lv
Department of Biochemistry and Molecular Biology, School of Life Sciences, Lanzhou University, 222 Tian Shui South Road, Lanzhou 730000, P.R. China. Electronic address: chenq@lzu.edu.cn.
Qiang
Q
Chen
D000804:Angiotensin II / Q000097:blood; D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology; D001794:Blood Pressure; D002216:Captopril / Q000494:pharmacology; D007022:Hypotension / Q000188:drug therapy / Q000209:etiology; D000860:Hypoxia / Q000150:complications / Q000503:physiopathology; D008297:Male; D009842:Oligopeptides / Q000494:pharmacology / Q000502:physiology* / Q000627:therapeutic use; D017208:Rats, Wistar; D012084:Renin-Angiotensin System; D012471:Salivary Proteins and Peptides / Q000494:pharmacology / Q000502:physiology* / Q000627:therapeutic use; D013777:Tetrazoles / Q000494:pharmacology; D014633:Valine / Q000031:analogs & derivatives / Q000494:pharmacology; D000068756:Valsartan
D000959:Antihypertensive Agents; D009842:Oligopeptides; D012471:Salivary Proteins and Peptides; D013777:Tetrazoles; C517879:glutaminyl-arginyl-phenylalanyl-seryl-arginine; D000804:Angiotensin II; D000068756:Valsartan; D002216:Captopril; D014633:Valine
AngII; Blood pressure; Hypoxia; Opiorphin; Vasopressor