UHPH-processed O/W submicron emulsions stabilised with a lipid-based surfactant: physicochemical characteristics and behaviour on in vitro TC7-cell monolayers and ex vivo pig's ear skin.
Submicron O/W emulsions formulated with sesame oil plus a lipid-base surfactant, and with or without retinyl acetate (RAC) as a model hydrophobic biomolecule, were prepared by single-pass homogenisation at ≥ 200 MPa (UHPH) and an initial fluid temperature (Tin) of 24°C. These emulsions were characterised by a monomodal distribution (peak maximum at 260 nm) and a 2-year potential physical stability at ambient temperature. Submicron droplets were investigated in term of (i) physicochemical characteristics (size distribution curves; ζ-potential value), and (ii) impact on TC7-cell monolayers (MTT-assay and cell LDH-leakage). Submicron droplets ± RAC did not affect or increased significantly (p=0.05) TC7-cell metabolic activity after 4-24h of exposure indicating absence of cellular impairment, except when high amounts of droplets were deposed on TC7-cells. Indeed, the lipid-based surfactant deposed alone on TC7-cells at high concentration, induced some significant (p=0.05) cell LDH-leakage, and therefore cell-membrane damage. Cellular uptake experiments revealed a significant (p=0.05) time-dependent internalisation of RAC from submicron droplets, and cellular transformation of RAC into retinol. The turnover of RAC into retinol and therefore RAC bioaccessibility appeared faster for RAC-micelles of similar size-range and prepared at atmospheric pressure with polysorbate 80, than for submicron O/W emulsions. Permeation experiments using pig's ear skin mounted on Franz-type diffusion cells, revealed RAC in dermis-epidermis, in significantly (p=0.05) higher amounts for submicron than coarse pre-emulsions. However, RAC amounts remained low for both emulsion-types and RAC was not detected in the receptor medium of Franz-type diffusion cells.
Université Montpellier 2, UMR 1208, Ingénierie des Agropolymères et Technologies Emergentes, Équipe de Biochimie et Technologie Alimentaires, CC023, Place Eugène Bataillon, 34095 Montpellier, Cedex 5, France.
Amal
A
Benzaria
Université Montpellier 2, UMR 1208, Ingénierie des Agropolymères et Technologies Emergentes, Équipe de Biochimie et Technologie Alimentaires, CC023, Place Eugène Bataillon, 34095 Montpellier, Cedex 5, France.
Alvar
A
Gràcia-Julià
Université Montpellier 2, UMR 1208, Ingénierie des Agropolymères et Technologies Emergentes, Équipe de Biochimie et Technologie Alimentaires, CC023, Place Eugène Bataillon, 34095 Montpellier, Cedex 5, France.
Laëtitia
L
Picart-Palmade
Université Montpellier 2, UMR 1208, Ingénierie des Agropolymères et Technologies Emergentes, Équipe de Biochimie et Technologie Alimentaires, CC023, Place Eugène Bataillon, 34095 Montpellier, Cedex 5, France.
Pauline
P
Hue
Université Montpellier 2, UMR 1208, Ingénierie des Agropolymères et Technologies Emergentes, Équipe de Biochimie et Technologie Alimentaires, CC023, Place Eugène Bataillon, 34095 Montpellier, Cedex 5, France.
Dominique
D
Chevalier-Lucia
Université Montpellier 1, IBMM-UMR 5247 UM1-UM2-CNRS, Avenue Charles Flahault-BP 14491, 34093 Montpellier, Cedex 5, France.
Gilberte
G
Marti-Mestres
NAHO COSMETICS, 270 rue Thomas Edison, 34400 Lunel, France.
Nadège
N
Hodor
Université Montpellier 2, UMR 1208, Ingénierie des Agropolymères et Technologies Emergentes, Équipe de Biochimie et Technologie Alimentaires, CC023, Place Eugène Bataillon, 34095 Montpellier, Cedex 5, France. Electronic address: Eliane.Dumay@univ-montp2.fr.
Eliane
E
Dumay
D000818:Animals; D018938:Caco-2 Cells; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D055598:Chemical Phenomena; D004431:Ear, External; D004655:Emulsions / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D008055:Lipids / Q000737:chemistry*; D009821:Oils / Q000737:chemistry*; D010316:Particle Size; D011312:Pressure; D012867:Skin / Q000166:cytology* / Q000378:metabolism; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry*; D013552:Swine; D014867:Water / Q000737:chemistry
D004655:Emulsions; D008055:Lipids; D009821:Oils; D013501:Surface-Active Agents; D014867:Water
In vitro TC7-cell cultures; Retinyl acetate uptake; Skin permeation; Submicron O/W emulsions; Ultra-high-pressure homogenisation