Characterization of a ginsenoside-transforming β-glucosidase from Paenibacillus mucilaginosus and its application for enhanced production of minor ginsenoside F₂.
A novel β-glucosidase (BglPm) was identified from Paenibacillus mucilaginosus KCTC 3870(T) which has ginsenoside converting activity. The gene, termed bglPm, consists of 1,260 bp and belongs to glycoside hydrolase family 1 (GH1). After being overexpressed and purified from Escherichia coli, the enzymatic properties of BglPm were investigated. The enzyme exhibited an optimal activity at 45°C and pH 7.5 and showed high bioconversion ability for major ginsenoside Rb1 and Rd into ginsenoside F2. Thus, it was used for mass production of relatively high pure F2 from relatively abundant protopanaxadiol type ginsenosides mixture (PPDGM) with combined usage of ginsenoside Rc-hydrolyzing enzyme. Scale-up of production using 250 g of the PPDGM resulted in 152 g of F2 with 80.1% chromatography purity and 95.7% recovery. These results suggest that this enzyme would be useful in the preparation of pharmacologically active ginsenoside F2 in the functional food and pharmaceutical industries.
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon, Republic of Korea.
Chang-Hao
CH
Cui
KAIST Institute for Biocentury, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon, Republic of Korea.
Jin-Kwang
JK
Kim
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon, Republic of Korea ; KAIST Institute for Biocentury, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon, Republic of Korea ; Intelligent Synthetic Biology Center, Yuseong-gu, Daejeon, Republic of Korea.
Sun-Chang
SC
Kim
KAIST Institute for Biocentury, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon, Republic of Korea.
Wan-Taek
WT
Im
D001709:Biotechnology / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D036145:Ginsenosides / Q000096:biosynthesis / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D056507:Paenibacillus / Q000201:enzymology*; D013696:Temperature; D001617:beta-Glucosidase / Q000235:genetics* / Q000378:metabolism
D017931:DNA Primers; D036145:Ginsenosides; C407883:ginsenoside F2; D001617:beta-Glucosidase