Production of 2,3-butanediol from xylose by engineered Saccharomyces cerevisiae.
2,3-Butanediol (2,3-BD) production from xylose that is abundant in lignocellulosic hydrolyzate would make the production of 2,3-BD more sustainable and economical. Saccharomyces cerevisiae can produce only trace amounts of 2,3-BD, but also cannot ferment xylose. Therefore, it is necessary to introduce both 2,3-BD production and xylose assimilation pathways into S. cerevisiae for producing 2,3-BD from xylose. A pyruvate decarboxylase (Pdc)-deficient mutant (SOS4) was used as a host in order to increase carbon flux toward 2,3-BD instead of ethanol. The XYL1, XYL2, and XYL3 genes coding for xylose assimilating enzymes derived from Scheffersomyces stipitis were introduced into the SOS4 strain to enable xylose utilization. Additionally, the alsS and alsD genes from Bacillus subtilis and endogenous BDH1 gene were overexpressed to increase 2,3-BD production from xylose. As a result, the resulting strain (BD4X) produced 20.7g/L of 2,3-BD from xylose with a yield of 0.27g 2,3-BD/g xylose. The titer of 2,3-BD from xylose increased up to 43.6g/L under a fed-batch fermentation. The BD4X strain produced (R, R)-2,3-BD dominantly (>97% of the total 2,3-BD) with trace amounts of meso-2,3-BD. These results suggest that S. cerevisiae might be a promising host for producing 2,3-BD from lignocellulosic biomass for industrial applications.
Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, Seoul 151-921, Republic of Korea.
Soo-Jung
SJ
Kim
Department of Food Science and Human Nutrition, and Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Seung-Oh
SO
Seo
Department of Advanced Fermentation Fusion Science and Technology, Kookmin University, Seoul 136-702, Republic of Korea.
Yong-Cheol
YC
Park
Department of Food Science and Human Nutrition, and Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Yong-Su
YS
Jin
Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, Seoul 151-921, Republic of Korea. Electronic address: jhseo94@snu.ac.kr.
Jin-Ho
JH
Seo
D002072:Butylene Glycols / Q000378:metabolism*; D005285:Fermentation; D060847:Metabolic Engineering / Q000379:methods*; D053858:Metabolic Networks and Pathways / Q000235:genetics; D009154:Mutation; D011767:Pyruvate Decarboxylase / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D014994:Xylose / Q000378:metabolism*
D002072:Butylene Glycols; D029701:Saccharomyces cerevisiae Proteins; C026978:2,3-butylene glycol; D014994:Xylose; D011767:Pyruvate Decarboxylase
2,3-Butanediol (2,3-BD); Pyruvate decarboxylase-deficient mutant; Saccharomyces cerevisiae; Xylose