[Study on determination method for components in monocrotalinum liposomes and their entrapment efficiency].
To establish an HPLC method for determining components in monocrotalinum liposomes. The results showed a good linear relationship in monocrotalinum liposomes within the concentration range between 1.6-102.4 mg x L(-1) (r = 0.999 8), with RSDs of intra-day precision, inter-day precision, stability and reproducibility of 0.61%, 0.92%, 1.7%, 1.6%, respectively. The recovery rate of monocrotaline was (99.96 +/- 0.50)%. These data indicated that the HPLC method could accurately determine components in monocrotalinum liposomes. Meanwhile, the microcolumn centrifugation method was established to determine the entrapment efficiency of components in monocrotalinum liposomes. As a result, the recovery rate and the blank liposome recovery of free components were (94.44 +/- 0.77)%, (95.86 +/- 0.68 )%, respectively. According to the parallel determination of the entrapment efficiency of three monocrotaline liposomes, their RSD was 4.0%. The data indicated that the microcolumn centrifugation method was an accurate and feasible method for determining the entrapment efficiency of monocrotaline liposomes.
School of Pharmacy, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, China. xyxnb@126.com
Yang
Y
Xiong
School of Pharmacy, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, China.
De-Jun
DJ
Zhang
School of Pharmacy, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, China.
Qing-Fang
QF
Xue
School of Pharmacy, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, China.
Sheng-Wu
SW
Huang
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004337:Drug Carriers / Q000737:chemistry*; D004339:Drug Compounding; D008081:Liposomes / Q000737:chemistry*; D016686:Monocrotaline / Q000737:chemistry*
D004337:Drug Carriers; D008081:Liposomes; D016686:Monocrotaline