Interactions of ruthenium complexes containing indoloquinoline moiety with human telomeric G-quadruplex DNA.
G-quadruplex structures are attractive targets for the development of anticancer drugs, as their formation in human telomere could impair telomerase activity, thus inducing apoptosis in cancer cells. Vast majority of G-quadruplex binding molecules have been designed and synthesized. Ruthenium complexes have also been reported to induction or stabilization of G-quadruplex structure of human telomeric sequence, whereas most of them generally promote the formation of antiparallel or hybrid-type G-quadruplex structure. Ruthenium complex that selectively promotes the formation of parallel G-quadruplex structure has rarely been reported. We reported here the interaction of two ruthenium complexes [Ru(bpy)2(mitatp)](2+)1 and [Ru(phen)2(mitatp)](2+)2 (bpy=2,2' bipyridine, phen=1,10-phenanthroline, mitatp=5-methoxy-isatino[1,2-b]-1,4,8,9-tetraazatriphenylene) containing indoloquinoline moiety with human telomeric G-quadruplex DNA (Telo22). Complex 1 binds to Telo22 tightly via a stable π-π stacking interaction and efficiently stabilizes the G-quadruplex structure. Circular dichroism (CD) spectra titration results suggest that complex 1 could induce Telo22 to fold into antiparallel G-quadruplex conformation. Complex 2 exhibits moderate G-quadruplex binding and stabilizing ability, while CD titration data reveals that complex 2 could promote the formation of parallel G-quadruplex structure.
School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, PR China. Electronic address: xiaoheiyu79@163.com.
Hui-juan
HJ
Yu
School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, PR China. Electronic address: gych@gdut.edu.cn.
Lin
L
Yu
School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, PR China.
Zhi-feng
ZF
Hao
School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, PR China.
Ying
Y
Zhao
D065966:Absorption, Physicochemical; D002942:Circular Dichroism; D056831:Coordination Complexes / Q000737:chemistry*; D005410:Flame Ionization; D031541:Fluorescence Resonance Energy Transfer; D054856:G-Quadruplexes; D006576:Heterocyclic Compounds, 4 or More Rings / Q000737:chemistry*; D006801:Humans; D049449:Luminescence; D010618:Phenanthrolines / Q000737:chemistry*; D011804:Quinolines / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry*; D016615:Telomere / Q000378:metabolism*; D017186:Titrimetry; D044366:Transition Temperature
D056831:Coordination Complexes; D006576:Heterocyclic Compounds, 4 or More Rings; D010618:Phenanthrolines; D011804:Quinolines; D012428:Ruthenium
G-quadruplex; Indoloquinoline; Ruthenium complex; Telomere