Acetylcholinesterase inhibitory activity of Haplophyton cimicidum.
In Oaxaca, México, Haplophyton cimicidum is used in combination with other plants as part of a folk strategy against arthropod pests of crops and cattle. Methanolic and crude alkaloid (acid-base extraction) extracts of H. cimicidum leaves were analysed by thin-layer chromatography (TLC) and assayed in vitro for acetylcholinesterase (AChE) inhibitory activity using Spodoptera frugiperda homogenates as source of enzyme. Derivatised chromatographic plates indicated the presence of indoles and alkaloids in the extracts. The crude alkaloid extract exhibited a higher number of compounds than the methanolic extract as judged by the number of spots on TLC plates. The crude alkaloid extract had a weak inhibition potential of AChE with a lower IC50 (93 μg mL(-1)) than the methanolic extract (159 μg mL(-1)). Indole alkaloids may be responsible for the activity, though a subsequent analysis of the extract components is necessary to determine the active alkaloid(s).
a Departamento de Ecología y Recursos Naturales , Facultad de Ciencias, Universidad Nacional Autónoma de México , México City , Mexico.
R E
RE
Llanos-Romero
R
R
Cárdenas
B
B
Zúñiga
J
J
Herrera-Santoyo
P
P
Guevara-Fefer
D000818:Animals; D029062:Apocynaceae / Q000737:chemistry*; D002417:Cattle; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D002855:Chromatography, Thin Layer; D004305:Dose-Response Relationship, Drug; D026121:Indole Alkaloids / Q000737:chemistry / Q000494:pharmacology*; D008800:Mexico; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D018411:Spodoptera / Q000201:enzymology
D002800:Cholinesterase Inhibitors; D026121:Indole Alkaloids; D010936:Plant Extracts
Apocynaceae; Haplophyton cimicidum; alkaloids; cholinesterase inhibition; thin layer chromatography