Quantification of vitamin A in fortified rapeseed, groundnut and soya oils using a simple portable device: comparison to high performance liquid chromatography.
Vitamin A deficiency is still one of the major public health problems in least developed countries. Fortification of vegetable oils is a strategy implemented worldwide to prevent this deficiency. For a fortification program to be effective, regular monitoring is necessary to control food quality in the producing units. The reference methods for vitamin A quantification are expensive and time-consuming. A rapid method should be useful for regular assessment of vitamin A in the oil industry. A portable device was compared to high-performance liquid chromatography (HPLC) for three plant oils (rapeseed, groundnut, and soya). The device presented a good linearity from 3 to 30 mg retinol equivalents per kg (mg RE.kg- 1). Its limits of detection and quantification were 3 mg RE.kg- 1 for groundnut and rapeseed oils and 4 mg RE.kg- 1 for soya oil. The intra-assay precision ranged from 1.48 % to 3.98 %, considered satisfactory. Accuracy estimated by the root mean squares error ranged from 3.99 to 5.49 and revealed a lower precision than HPLC (0.4 to 2.25). Although it offers less precision than HPLC, the device estimates quickly the vitamin A content of the tested oils from 3 or 4 to 15 mg RE.kg- 1.
IRD; UMR 204, IRD / Montpellier1 / Montpellier2 / SupAgro (NUTRIPASS), Montpellier, France.
Cécile
C
Renaud
IRD; UMR 204, IRD / Montpellier1 / Montpellier2 / SupAgro (NUTRIPASS), Montpellier, France.
Jacques
J
Berger
GAIN - Global Alliance for Improved Nutrition, Geneva, Switzerland.
Arnaud
A
Laillou
Montpellier SupAgro; UMR 204, IRD / Montpellier1 / Montpellier2 / SupAgro (NUTRIPASS), Montpellier, France.
Sylvie
S
Avallone
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005229:Fatty Acids, Monounsaturated; D005527:Food, Fortified / Q000032:analysis; D057230:Limit of Detection; D000074241:Peanut Oil; D010938:Plant Oils / Q000032:analysis*; D000074262:Rapeseed Oil; D011933:Reagent Kits, Diagnostic; D012639:Seeds / Q000737:chemistry; D013024:Soybean Oil / Q000032:analysis*; D014801:Vitamin A / Q000032:analysis*
D005229:Fatty Acids, Monounsaturated; D000074241:Peanut Oil; D010938:Plant Oils; D000074262:Rapeseed Oil; D011933:Reagent Kits, Diagnostic; D014801:Vitamin A; D013024:Soybean Oil
food quality; retinyl palmitate; vitamin A fortification