Analysis of lysozyme in cheese samples by on-line combination of capillary zone electrophoresis and mass spectrometry.
Some methodological aspects of an on-line combination of capillary zone electrophoresis with mass spectrometric detection (CZE-QqQ-MS) were studied in this work as well as the possibilities of using this combination for analysis of the high-molecular mass compounds present in multi-component matrices. All experiments using an on-line combination of capillary electrophoresis with mass spectrometric detection were carried out in cationic mode in covalently-coated capillary. The optimised electrolyte system consisted of 100 mmol/L formic acid. Prior to the CZE-QqQ-MS analysis, an extraction of lysozyme from cheese samples using 1 mol/L of acetic acid was performed. The LOD was 3.6 mg lysozyme per kg and the LOQ was 10.9 mg lysozyme per kg. The concentration range of the lysozyme determined in four cheese samples analysed in this work was from 0.5 to 3.3g of lysozyme per kg. The values of the relative standard deviations thus obtained were from 4.6% to 9.3% depending on the cheese sample.
Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská Dolina CH-2, SK-842 15 Bratislava, Slovak Republic.
Monika
M
Kondeková
Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Natural Science, Palacký University Olomouc, 17. listopadu 12, 771 46 Olomouc, Czech Republic.
Vítězslav
V
Maier
Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Natural Science, Palacký University Olomouc, 17. listopadu 12, 771 46 Olomouc, Czech Republic.
Pavlína
P
Ginterová
Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská Dolina CH-2, SK-842 15 Bratislava, Slovak Republic. Electronic address: marak@fns.uniba.sk.
Jozef
J
Marák
Department of Analytical Chemistry, Faculty of Natural Science, Palacký University Olomouc, 17. listopadu 12, 771 46 Olomouc, Czech Republic.
Juraj
J
Sevčík
D000818:Animals; D002611:Cheese / Q000032:analysis*; D002645:Chickens; D004527:Egg Proteins / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005520:Food Preservatives / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D009113:Muramidase / Q000032:analysis*
D004527:Egg Proteins; D005520:Food Preservatives; D009113:Muramidase
Capillary zone electrophoresis; Cheese; Lysozyme; Mass spectrometry; Multi-component matrices