Effects of 1,25(OH)2D3 in immune response regulation of systemic lupus erithematosus (SLE) patient with hypovitamin D.
Vitamin D deficiency has been associated with pathogenesis of autoimmune diseases including SLE; however, there were still lack of data about the effects of administration of vitamin D in immune regulation in SLE patients. The aim of this study was to investigate the effects of calcitriol/1,25(OH)2D3 on dendritic cells maturation and Th17 and Treg cells activation in SLE patients with hypovitamin D. The monocytes and lymphocytes of five SLE patients with hypovitamin D were divided into 4 groups, P0 (0 nM/control), P1 (1 nM), P2 (10 nM), and P3 (100 nM) as cultured samples. Flowcytometry analysis was used to evaluate dendritic cell maturation (the percentage of CD40, CD86, and HLA-DR expression) and the amount of Th17 and Treg cells (the percentage of Th17 and Treg cells). Cytokines production of IL-12, IL-17A, and TGF-β measured by ELISA. This study showed significant differences in CD40, CD86, HLA-DR expressions, and Th17 percentage in 10 nM of 1,25(OH)2D3 compared to that of control. For cytokines secretion, there was also significant difference between IL-12p70 and IL-17A levels in 10 nM of 1,25(OH)2D3 compared to that of control. The 1,25(OH)2D3 increased Treg cells and TGF-β level but not significant. Our study concluded that 1,25(OH)2D3 inhibited dendritic cells maturation and Th17 cells activation in SLE patients. The 1,25(OH)2D3 increased Treg cells but not significant.
Division of Rheumatology, Department of Internal Medicine, Faculty of Medicine Brawijaya University, Saiful Anwar General Hospital Malang, Indonesia.
Cesarius S
CS
Wahono
Biomedical Sciences Master Program, Faculty of Medicine Brawijaya University Malang, Indonesia ; Department of Pharmacology, Faculty of Medicine, Malahayati University Lampung, Indonesia.
Hetti
H
Rusmini
Biomedical Sciences Master Program, Faculty of Medicine Brawijaya University Malang, Indonesia.
Dwi
D
Soelistyoningsih
Biomedical Sciences Master Program, Faculty of Medicine Brawijaya University Malang, Indonesia ; Laboratory of Clinical Skill, Faculty of Medicine, Malang Islamic University Malang, Indonesia.
Reza
R
Hakim
Department of Clinical Pathology, Faculty of Medicine Brawijaya University, Saiful Anwar General Hospital Malang, Indonesia.
Kusworini
K
Handono
Department of Parasitology, Faculty of Medicine Brawijaya University Malang, Indonesia.
Agustina T
AT
Endharti
Division of Rheumatology, Department of Internal Medicine, Faculty of Medicine Brawijaya University, Saiful Anwar General Hospital Malang, Indonesia.
Handono
H
Kalim
Department of Clinical Pathology, Faculty of Medicine Brawijaya University, Saiful Anwar General Hospital Malang, Indonesia.
Edi
E
Widjajanto
25(OH)2D3]; Calcitriol [1; Th17 cells; Treg cells; dendritic cell; systemic lupus erythematosus