[Does bilirubin interfere with capillary electrophoresis of serum proteins?].
Capillary electrophoresis of serum proteins is a fast, reliable and simple technique, but many interference exist. The objective of our work is to study the interference of bilirubin on this technique; 70 icteric sera were analysed on Capillarys ™ (Sebia). A second electrophoresis was performed on 40 samples after bilirubin photodegradation. The bilirubin and serum proteins were determinated respectively by Jendrassik and Grof and biuret methods on Konélab 20i ™ (Thermo Electron Corporation). We found abnormal spreading of the albumin fraction of the anode side wich constitute sometimes an isolated fraction in the traditional area of pre-albumin migration. This fraction varies from 2.0 ± 2.0% (0.0 to 7.3%) or 0.98 ± 1.53 g/L (0 to 5.3 g/L) and it seems to be related to the direct bilirubin since, following overloading sera with a solution of bilirubin, no further fraction was recovered. An average decrease of bilirubin after photodegradation of 58 ± 17% (26-89%) is followed by a decrease in the same order 64 ± 38% (10-100%) of the additional fraction. Acetate cellulose electrophoresis of the same samples showed no variation. The high bilirubin levels seem modify slightly the electrophoretic profile. However the impact of the interference on the interpretation of electrophoretic trace is negligible.
Laboratoire de biochimie-toxicologie, CHU Fattouma Bourguiba, Monastir, Tunisie.
Ilhem
I
Hellara
Laboratoire de biochimie-toxicologie, CHU Fattouma Bourguiba, Monastir, Tunisie.
Ons
O
Fekih
Laboratoire de biochimie-toxicologie, CHU Fattouma Bourguiba, Monastir, Tunisie.
Sonia
S
Triki
Laboratoire de biochimie-toxicologie, CHU Fattouma Bourguiba, Monastir, Tunisie.
Ahlem
A
Elmay
Laboratoire de biochimie-toxicologie, CHU Fattouma Bourguiba, Monastir, Tunisie.
Fadoua
F
Neffati
Laboratoire de biochimie-toxicologie, CHU Fattouma Bourguiba, Monastir, Tunisie.
Mohamed Fadhel
MF
Najjar
D000418:Albumins / Q000032:analysis; D001663:Bilirubin / Q000097:blood*; D001774:Blood Chemical Analysis / Q000379:methods*; D001798:Blood Proteins / Q000032:analysis*; D005591:Chemical Fractionation; D019075:Electrophoresis, Capillary; D006801:Humans
D000418:Albumins; D001798:Blood Proteins; D001663:Bilirubin
bilirubin; capillary electrophoresis; interference; proteins