Methanol Extract of Goat's-beard (Aruncus dioicus) Reduces Renal Injury by Inhibiting Apoptosis in a Rat Model of Ischemia-Reperfusion.
Interruption or prolonged reduction and subsequent restoration of blood flow into the kidney triggers the generation of a burst of reactive oxygen species (ROS), leading to injury in the tubular epithelial cells. In this study, we determined whether methanol extract of goat's-beard (Aruncus dioicus) (extract) could prevent this ischemia/re-perfusion injury. When in vitro radical scavenging activity of the extract was measured using a DPPH radical quenching assay, the extract displayed slightly lower activity than ascorbic acid. One hour after administration of the extract (400 mg/kg) by intraperitoneal injection in rats, renal ischemia/reperfusion injury was generated by clamping the left renal artery for forty minutes, followed by 24 hr restoration of blood circulation. Prior to clamping the left renal artery, the right renal artery was removed. Compared with the vehicle-treated group, pre-treatment with the extract significantly reduced the tubular epithelial cell injury by 37% in the outer medulla region, and consequently reduced serum creatinine concentration by 39%. Reduction in the cell injury was mediated by attenuation of Bax/Bcl-2 ratio, inhibition of caspase-3 activation from procaspase-3, and subsequent reduction in the number of apoptotic cells. Thus, goat's-beard (Aruncus dioicus) might be developed as a prophylactic agent to prevent acute kidney injury.
Department of Biochemistry, School of Medicine, Catholic University of Daegu, Daegu 705-718, Korea.
Hae Sook
HS
Baek
Department of Biochemistry, School of Medicine, Catholic University of Daegu, Daegu 705-718, Korea.
Sun Ha
SH
Lim
Department of Internal Medicine, School of Medicine, Catholic University of Daegu, Daegu 705-718, Korea.
Ki Sung
KS
Ahn
Department of Biochemistry, School of Medicine, Catholic University of Daegu, Daegu 705-718, Korea.
Jongwon
J
Lee
apoptosis; goat’s-beard; ischemia/reperfusion; kidney; methanol extract; reactive oxygen species