Genome-wide miRNA-profiling of aflatoxin B1-induced hepatic injury using deep sequencing.
Aflatoxin B1 is a potent carcinogen which can induce** hepatocellular carcinoma (HCC) in mammals. Though microRNAs are known to play important roles in tumorigenesis, the functional complexity of microRNAs in AFB1-induced hepatocellular tumorigenesis has not yet been elucidated. Here, we applied Illumina deep sequencing technology for high-throughput profiling of microRNA in rat liver tissue before and after treatment with aflatoxin B1. Analysis of mature miRNAs from different arms of pre-miRNAs allowed us to identify the predominant form of miRNA. We studied the differential expression profile of miRNAs in two libraries, identifying several cancer-related microRNAs which exhibit abnormal expression. KEGG analysis indicated that predicted target genes of differentially expressed miRNAs are involved in cancer-related pathways. Bioinformatic analysis predicted 16 potential novel miRNAs. Our work provides new insights at the miRNA level into AFB1-induced hepatic injury which may lead to HCC.
The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, and the School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Weiqiang
W
Yang
The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, and the School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Junwei
J
Lian
The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, and the School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China; Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Youjun
Y
Feng
Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Swaminath
S
Srinivas
The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, and the School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Zhenni
Z
Guo
The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, and the School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Hong
H
Zhong
The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, and the School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Zhenhong
Z
Zhuang
The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, and the School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. Electronic address: wshyyl@sina.com.
Shihua
S
Wang
D016604:Aflatoxin B1 / Q000633:toxicity*; D000818:Animals; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000737:chemistry / Q000235:genetics; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D019295:Computational Biology; D030541:Databases, Genetic; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D005819:Genetic Markers; D059014:High-Throughput Nucleotide Sequencing; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008113:Liver Neoplasms / Q000139:chemically induced / Q000235:genetics; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008969:Molecular Sequence Data; D012322:RNA Precursors / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D015203:Reproducibility of Results
D005819:Genetic Markers; D035683:MicroRNAs; D012322:RNA Precursors; D016604:Aflatoxin B1
Aflatoxin B1; Hepatocellular carcinoma; High-throughput sequencing; MicroRNA