High-resolution molecular karyotyping uncovers pairing between ancestrally related Brassica chromosomes.
How do chromosomal regions with differing degrees of homology and homeology interact at meiosis? We provide a novel analytical method based on simple genetics principles which can help to answer this important question. This method interrogates high-throughput molecular marker data in order to infer chromosome behavior at meiosis in interspecific hybrids. We validated this method using high-resolution molecular marker karyotyping in two experimental Brassica populations derived from interspecific crosses among B. juncea, B. napus and B. carinata, using a single nucleotide polymorphism chip. This method of analysis successfully identified meiotic interactions between chromosomes sharing different degrees of similarity: full-length homologs; full-length homeologs; large sections of primary homeologs; and small sections of secondary homeologs. This analytical method can be applied to any allopolyploid species or fertile interspecific hybrid in order to detect meiotic associations. This genetic information can then be used to identify which genomic regions share functional homeology (i.e., retain enough similarity to allow pairing and segregation at meiosis). When applied to interspecific hybrids for which reference genome sequences are available, the question of how differing degrees of homology and homeology affect meiotic interactions may finally be resolved.
School of Agriculture and Food Sciences, The University of Queensland, Brisbane, 4072, Qld, Australia.|Centre for Integrative Legume Research, The University of Queensland, Brisbane, 4072, Qld, Australia.
Annaliese S
AS
Mason
School of Agriculture and Food Sciences, The University of Queensland, Brisbane, 4072, Qld, Australia.|Centre for Integrative Legume Research, The University of Queensland, Brisbane, 4072, Qld, Australia.
Jacqueline
J
Batley
School of Agriculture and Food Sciences, The University of Queensland, Brisbane, 4072, Qld, Australia.|Australian Centre for Plant Functional Genomics, The University of Queensland, Brisbane, 4072, Qld, Australia.
Philipp Emanuel
PE
Bayer
School of Agriculture and Food Sciences, The University of Queensland, Brisbane, 4072, Qld, Australia.|Centre for Integrative Legume Research, The University of Queensland, Brisbane, 4072, Qld, Australia.
Alice
A
Hayward
The UWA Institute of Agriculture, The University of Western Australia, 35 Stirling Highway, Crawley, 6009, WA, Australia.
Wallace A
WA
Cowling
The UWA Institute of Agriculture, The University of Western Australia, 35 Stirling Highway, Crawley, 6009, WA, Australia.|School of Plant Biology, The University of Western Australia, 35 Stirling Highway, Crawley, 6009, WA, Australia.
Matthew N
MN
Nelson
D000483:Alleles; D001937:Brassica / Q000235:genetics*; D019457:Chromosome Breakage; D023902:Chromosome Pairing / Q000235:genetics*; D020090:Chromosome Segregation / Q000235:genetics; D032461:Chromosomes, Plant / Q000235:genetics*; D003433:Crosses, Genetic; D015321:Gene Rearrangement / Q000235:genetics; D005819:Genetic Markers; D018745:Genome, Plant / Q000235:genetics; D006824:Hybridization, Genetic; D040582:Inheritance Patterns / Q000235:genetics; D007621:Karyotyping / Q000379:methods*; D010802:Phylogeny; D011995:Recombination, Genetic / Q000235:genetics; D013045:Species Specificity
D005819:Genetic Markers
Brassica; meiosis; molecular karyotyping; polyploidy; recombination