The interaction of DNA with phytoferritin during iron oxidation.
Phytoferritin from legume seeds is considered an iron supplement with great potential. Phytoferritin co-exists with plastid DNA in amyloplasts of legume seed cells where Fe(2+) is oxidized into Fe(3+), followed by storage within the inner cavity of the protein. In this study, the interaction of plasmid DNA with black bean (Phaseolus vulgaris L.) seed ferritin (BSF) during iron oxidation was studied. Results indicated that iron ions facilitated formation of apoBSF aggregates at a high iron loading (>48 Fe(2+)/shell). Interestingly, the co-existence of DNA and ferritin has a pronounced effect on iron uptake by ferritin. This view is confirmed by a pronounced increase in the rate of iron oxidation catalysed by apoBSF in the presence of DNA. On the other hand, the apoBSF exhibited a marked DNA-protective function against oxidative damage at a low loading of Fe(2+) (⩽ 48 Fe(2+)/shell). However, outside this ratio, such an effect gradually decreased, because the added iron exceeded the iron binding capacity of ferritin. The current study advances the understanding of the interaction among multi-components in foodstuffs.
CAU & ACC Joint-Laboratory of Space Food, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing Key Laboratory of Functional Food from Plant Resources, Beijing 100083, China.
Rui
R
Yang
CAU & ACC Joint-Laboratory of Space Food, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing Key Laboratory of Functional Food from Plant Resources, Beijing 100083, China.
Senpei
S
Yang
CAU & ACC Joint-Laboratory of Space Food, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing Key Laboratory of Functional Food from Plant Resources, Beijing 100083, China.
Xiayun
X
Liao
CAU & ACC Joint-Laboratory of Space Food, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing Key Laboratory of Functional Food from Plant Resources, Beijing 100083, China.
Jianjun
J
Deng
CAU & ACC Joint-Laboratory of Space Food, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing Key Laboratory of Functional Food from Plant Resources, Beijing 100083, China. Electronic address: gzhao@cau.edu.cn.
Guanghua
G
Zhao
D001052:Apoferritins / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D005293:Ferritins / Q000737:chemistry*; D007501:Iron / Q000737:chemistry*; D010084:Oxidation-Reduction; D027805:Phaseolus / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*
D010940:Plant Proteins; C030124:phytoferritin; D004247:DNA; D005293:Ferritins; D001052:Apoferritins; D007501:Iron
DNA-protective function; Ferritin association; Iron oxidation; Phytoferritin