TSG-6 mediates the effect of tendon derived stem cells for rotator cuff healing.
BACKGROUND
Bone marrow stem cells (MSCs) were able to reduce fibrovascular tissues formation via TNF alpha-stimulated gene/protein 6 (6TSG-6) in various animal models. At the same time, tendon-derived stem cells (TDSCs) were able to promote rotator cuff healing; however, the mechanism is still unknown.


OBJECTIVE
To investigate the role of TSG-6 in the treatment of rotator cuff healing with TDSCs.


METHODS
45 rats underwent unilateral detachment and repair of the supraspinatus tendon. 15 animals received TDSCs in a fibrin glue carrier(Group A), 15 received TSG-6 silenced TDSCs (Group B), and 15 received fibrin glue for control (Group C). Animals were sacrificed at 4 weeks and evaluated for the biomechanical testing. Statistical analysis was performed with an independent t test with significance set at p = 0.05.


RESULTS
The ultimate stress was greater in the TDSCs group (4.91 ± 1.41 N/mm(2)) as compared with the Control group (2.99 ± 1.04 N/mm(2)) (p < 0.05). However, when silent the expression of TSG-6, the TSG-6 silenced group (3.36 ± 0.96 N/mm(2)) showed no benefit over the control group (p = 0.32).


CONCLUSIONS
TSG-6 mediates the function of TDSCs to improve the structure and the attachment strength of the healing tendon-bone interface.
Department of Orthopedics, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China. zqywyx@163.com.
B
B
Cheng
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H
Ge
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Zhou
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Zhang
D000818:Animals; D001696:Biomechanical Phenomena / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D015718:Fibrin Tissue Adhesive / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D017006:Rotator Cuff / Q000378:metabolism*; D013234:Stem Cells / Q000378:metabolism*; D013710:Tendons / Q000378:metabolism*; D014945:Wound Healing / Q000502:physiology*
D015815:Cell Adhesion Molecules; D015718:Fibrin Tissue Adhesive; C513056:Tnfaip6 protein, rat