Evaluation of an automated SPF10-LiPA25 assay for detection and typing of human papillomavirus in archival samples.
An automated blotter was evaluated for detection of the HPV SPF10-LiPA25 RHA for HPV genotyping with 63 FFPE tissue and 45 WHO proficiency panel samples. The results showed that 10-20% more types were detected and bands were of significantly stronger intensity (p<0.0001) compared to manual processing. Therefore it was concluded that automated detection of HPV genotypes using the HPV SPF10-LiPA25 is more sensitive for low copy number and mixed-type HPV samples.
Regional HPV Labnet Reference Laboratory, Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Parkville, Victoria, Australia; Murdoch Children's Research Institute, Parkville, Victoria, Australia. Electronic address: alyssa.cornall@mcri.edu.au.
Alyssa M
AM
Cornall
University of Applied Sciences, Leiden, The Netherlands. Electronic address: wim.quint@gmail.com.
Wim H
WH
Quint
Regional HPV Labnet Reference Laboratory, Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Parkville, Victoria, Australia; Murdoch Children's Research Institute, Parkville, Victoria, Australia; Department of Obstetrics and Gynaecology, University of Melbourne, Victoria, Australia. Electronic address: suzanne.garland@thewomens.org.au.
Suzanne M
SM
Garland
Regional HPV Labnet Reference Laboratory, Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Parkville, Victoria, Australia; Murdoch Children's Research Institute, Parkville, Victoria, Australia; Department of Obstetrics and Gynaecology, University of Melbourne, Victoria, Australia. Electronic address: sepehr.tabrizi@thewomens.org.au.
Sepehr N
SN
Tabrizi
D057205:Automation, Laboratory / Q000379:methods*; D005260:Female; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D008297:Male; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology*; D014773:Virology / Q000379:methods*
Automation; FFPE; Genotyping; HPV; SPF10-LiPA