TMA vessel segmentation based on color and morphological features: application to angiogenesis research.
Given that angiogenesis and lymphangiogenesis are strongly related to prognosis in neoplastic and other pathologies and that many methods exist that provide different results, we aim to construct a morphometric tool allowing us to measure different aspects of the shape and size of vascular vessels in a complete and accurate way. The developed tool presented is based on vessel closing which is an essential property to properly characterize the size and the shape of vascular and lymphatic vessels. The method is fast and accurate improving existing tools for angiogenesis analysis. The tool also improves the accuracy of vascular density measurements, since the set of endothelial cells forming a vessel is considered as a single object.
VISILAB, E.T.S.I. Industriales, Universidad de Castilla-La Mancha, 13071 Ciudad Real, Spain.
M Milagro
MM
Fernández-Carrobles
Fundación Investigación Clínico de Valencia, Instituto de Investigación Sanitaria, INCLIVA, 46010 Valencia, Spain.
Irene
I
Tadeo
VISILAB, E.T.S.I. Industriales, Universidad de Castilla-La Mancha, 13071 Ciudad Real, Spain.
Gloria
G
Bueno
Laboratorio de Patología Molecular, Departmento de Patología, Facultad de Medicina y Odontología, Universidad de Valencia, 46010 Valencia, Spain.
Rosa
R
Noguera
VISILAB, E.T.S.I. Industriales, Universidad de Castilla-La Mancha, 13071 Ciudad Real, Spain.
Oscar
O
Déniz
VISILAB, E.T.S.I. Industriales, Universidad de Castilla-La Mancha, 13071 Ciudad Real, Spain.
Jesús
J
Salido
Departmento de Anatomía Patológica, Hospital General Universitario de Ciudad Real, 13005 Ciudad Real, Spain.
Marcial
M
García-Rojo
D000818:Animals; D001808:Blood Vessels / Q000378:metabolism / Q000473:pathology; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods; D042601:Lymphatic Vessels / Q000378:metabolism / Q000473:pathology; D009369:Neoplasms / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000473:pathology