Nucleotide sequencing and identification of some wild mushrooms.
The rDNA-ITS (Ribosomal DNA Internal Transcribed Spacers) fragment of the genomic DNA of 8 wild edible mushrooms (collected from Eastern Chota Nagpur Plateau of West Bengal, India) was amplified using ITS1 (Internal Transcribed Spacers 1) and ITS2 primers and subjected to nucleotide sequence determination for identification of mushrooms as mentioned. The sequences were aligned using ClustalW software program. The aligned sequences revealed identity (homology percentage from GenBank data base) of Amanita hemibapha [CN (Chota Nagpur) 1, % identity 99 (JX844716.1)], Amanita sp. [CN 2, % identity 98 (JX844763.1)], Astraeus hygrometricus [CN 3, % identity 87 (FJ536664.1)], Termitomyces sp. [CN 4, % identity 90 (JF746992.1)], Termitomyces sp. [CN 5, % identity 99 (GU001667.1)], T. microcarpus [CN 6, % identity 82 (EF421077.1)], Termitomyces sp. [CN 7, % identity 76 (JF746993.1)], and Volvariella volvacea [CN 8, % identity 100 (JN086680.1)]. Although out of 8 mushrooms 4 could be identified up to species level, the nucleotide sequences of the rest may be relevant to further characterization. A phylogenetic tree is constructed using Neighbor-Joining method showing interrelationship between/among the mushrooms. The determined nucleotide sequences of the mushrooms may provide additional information enriching GenBank database aiding to molecular taxonomy and facilitating its domestication and characterization for human benefits.
Cytogenetics, Genetics and Plant Breeding Section, Department of Botany, Kalyani University, Kalyani, West Bengal 741235, India.
Sudip Kumar
SK
Das
Cytogenetics, Genetics and Plant Breeding Section, Department of Botany, Kalyani University, Kalyani, West Bengal 741235, India.
Aninda
A
Mandal
Cytogenetics, Genetics and Plant Breeding Section, Department of Botany, Kalyani University, Kalyani, West Bengal 741235, India.
Animesh K
AK
Datta
Pteridology-Palaeobotany Section, Department of Botany, Kalyani University, Kalyani, West Bengal 741235, India.
Sudha
S
Gupta
Department of Botany, Charuchandra College, Kolkata, West Bengal 700029, India.
Rita
R
Paul
Department of Botany, Narasinha Dutt College, Howrah, West Bengal 711101, India.
Aditi
A
Saha
P.G. Department of Botany, Hooghly Mohsin College, Hooghly, West Bengal 712101, India.
Sonali
S
Sengupta
Cytogenetics, Genetics and Plant Breeding Section, Department of Botany, Kalyani University, Kalyani, West Bengal 741235, India.
Priyanka Kumari
PK
Dubey
D000363:Agaricales / Q000145:classification* / Q000235:genetics*; D001483:Base Sequence; D019295:Computational Biology; D004271:DNA, Fungal; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal