Deep UV generation and direct DNA photo-interaction by harmonic nanoparticles in labelled samples.
A biophotonics approach based on the nonlinear optical process of second harmonic generation is presented and demonstrated on malignant human cell lines labelled by harmonic nanoparticles. The method enables independent imaging and therapeutic action, selecting each modality by simply tuning the excitation laser wavelength from infrared to visible. In particular, the generation of deep ultraviolet radiation at 270 nm allows direct interaction with nuclear DNA in the absence of photosensitizing molecules.
Institute of Chemical Sciences and Engineering, EPFL, CH-1015, Lausanne, Switzerland.
Davide
D
Staedler
Thibaud
T
Magouroux
Solène
S
Passemard
Sebastian
S
Schwung
Marc
M
Dubled
Guillaume Stéphane
GS
Schneiter
Daniel
D
Rytz
Sandrine
S
Gerber-Lemaire
Luigi
L
Bonacina
Jean-Pierre
JP
Wolf
D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D017785:Photons; D017319:Photosensitizing Agents / Q000737:chemistry / Q000633:toxicity; D017382:Reactive Oxygen Species / Q000378:metabolism; D014466:Ultraviolet Rays
D017319:Photosensitizing Agents; D017382:Reactive Oxygen Species; D004247:DNA