RNA interference targeting extracellular matrix metalloproteinase inducer (CD147) inhibits growth and increases chemosensitivity in human cervical cancer cells.
Overexpression of extracellular matrix metalloproteinase (MMP) inducer (EMMPRIN CD147) has been implicated in the growth and survival of malignant cells. However, its presence and role in cervical cancer cells has not been well-studied. In the present study, small interfering RNA (siRNA) was designed and synthesized to breakdown the expression of CD147. The present data demonstrated that 24 and 48 hours after transfecting CD147 siRNA, both the CD147 mRNA and protein expression were significantly inhibited as determined by quantitative real-time polymerase chain reaction (RT-PCR) and immunocytochemistry. Meanwhile, simultaneous silencing of CD147 resulted in distinctly increasing MMP-9, VEGF, and MDR-1. Further studies demonstrated decreased CD147 expression, resulted in G1/S phase transition with flow cytometry analysis, as well as the resistance of the cells to 5-FU. These findings provide further evidence that CD147 may become a promising therapeutic target for human cervical cancer and a potential chemotherapy-sensitizing agent.
School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, China.
F
F
Zhang
School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, China.
Y L
YL
Zeng
School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, China.
X G
XG
Zhang
School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, China.
W J
WJ
Chen
Cancer Hospital of Gansu Province, Lanzhou, China.
R
R
Yang
School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, China.
S J
SJ
Li
D051926:Basigin / Q000235:genetics*; D049109:Cell Proliferation; D005260:Female; D006367:HeLa Cells; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000628:therapy*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
C497886:BSG protein, human; D034741:RNA, Small Interfering; D042461:Vascular Endothelial Growth Factor A; D051926:Basigin; D020780:Matrix Metalloproteinase 9