[Expression and significance of lymphocytes in chronic rhinosinusitis].
OBJECTIVE
To investigate the B lymphocytes(CD20) and T-lymphocyte subsets (CD4, CD8) expression and clinical significance in chronic rhinosinusitis.


METHODS
Immunohistochemical technique was applied to detect the expression of the B lymphocytes (CD20), helper T lymphocytes (CD4) and the cytotoxic T lymphocyte (CD8) in 15 patients with chronic rhinosinusitis without nasal polyps group (CRSsNP). 12 patients of chronic nasal sinusitis with nasal polyp (CRSwNP), 7 cases of recurrent CRSwNP group and 13 patients with inferior turbinate mucosa in the control group. The Mann-Whitney U test was used to analyze CD)20, CD4 and CD8 between the experimental groups and the control group. One- Way ANOVA was used to compare the lymphocyte infiltration between the experiment groups.


RESULTS
Patients with CRS had higher infiltration of the B lymphocytes (CD20) and T lymphocyte subsets (CD4, CD88) than the patients of the control group (P < 0.05). The expression of T lymphocyte subsets (CD4, CD8) in CRSwNP and recurrent CRSwNP groups was significantly higher than in CRSsNP (P < 0.05). The expression of CD4 in the recurrent CRSwNP group was obviously higher than that in CRSsNP and CRSwNP (P < 0.01).


CONCLUSIONS
The B lymphocytes, helper T cells and cytotoxic T cells have the high expression in CRS groups, which involved with the formation of inflammation. Furthermore nasal polyps are closely related to T lymphocyte infiltration.
Department of Otorhinolaryngology Head and Neck Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China.
Jing
J
Du
Department of Otorhinolaryngology Head and Neck Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China.
Chuyuan
C
Zhao
Department of Otorhinolaryngology Head and Neck Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China.
Xin
X
Wang
D000293:Adolescent; D000328:Adult; D001402:B-Lymphocytes / Q000166:cytology*; D016022:Case-Control Studies; D002648:Child; D002908:Chronic Disease; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000150:complications; D012220:Rhinitis / Q000150:complications / Q000276:immunology*; D012852:Sinusitis / Q000150:complications / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000166:cytology*; D055815:Young Adult