Glassy carbon electrodes sequentially modified by cysteamine-capped gold nanoparticles and poly(amidoamine) dendrimers generation 4.5 for detecting uric acid in human serum without ascorbic acid interference.
Glassy carbon electrodes (GCE) were sequentially modified by cysteamine-capped gold nanoparticles (AuNp@cysteamine) and PAMAM dendrimers generation 4.5 bearing 128-COOH peripheral groups (GCE/AuNp@cysteamine/PAMAM), in order to explore their capabilities as electrochemical detectors of uric acid (UA) in human serum samples at pH 2. The results showed that concentrations of UA detected by cyclic voltammetry with GCE/AuNp@cysteamine/PAMAM were comparable (deviation <±10%; limits of detection (LOD) and quantification (LOQ) were 1.7×10(-4) and 5.8×10(-4) mg dL(-1), respectively) to those concentrations obtained using the uricase-based enzymatic-colorimetric method. It was also observed that the presence of dendrimers in the GCE/AuNp@cysteamine/PAMAM system minimizes ascorbic acid (AA) interference during UA oxidation, thus improving the electrocatalytic activity of the gold nanoparticles.
Centro de Investigación y Desarrollo Tecnológico en Electroquímica S.C., Parque Tecnológico Querétaro s/n, Sanfandila, Pedro Escobedo 76703, Qro, Mexico; Departamento de Química, División de Ciencias Naturales y Exactas, Campus Guanajuato, Universidad de Guanajuato, Noria Alta s/n, Guanajuato 36050, Gto, Mexico.
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Ramírez-Segovia
Centro de Investigación y Desarrollo Tecnológico en Electroquímica S.C., Parque Tecnológico Querétaro s/n, Sanfandila, Pedro Escobedo 76703, Qro, Mexico.
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JA
Banda-Alemán
Departamento de Química, División de Ciencias Naturales y Exactas, Campus Guanajuato, Universidad de Guanajuato, Noria Alta s/n, Guanajuato 36050, Gto, Mexico.
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Gutiérrez-Granados
Centro de Investigación y Desarrollo Tecnológico en Electroquímica S.C., Parque Tecnológico Querétaro s/n, Sanfandila, Pedro Escobedo 76703, Qro, Mexico.
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A
Rodríguez
Centro de Investigación y Desarrollo Tecnológico en Electroquímica S.C., Parque Tecnológico Querétaro s/n, Sanfandila, Pedro Escobedo 76703, Qro, Mexico.
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Rodríguez
Centro de Investigación y Desarrollo Tecnológico en Electroquímica S.C., Parque Tecnológico Querétaro s/n, Sanfandila, Pedro Escobedo 76703, Qro, Mexico.
Luis A
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Godínez
Centro de Investigación y Desarrollo Tecnológico en Electroquímica S.C., Parque Tecnológico Querétaro s/n, Sanfandila, Pedro Escobedo 76703, Qro, Mexico.
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Bustos
Centro de Investigación y Desarrollo Tecnológico en Electroquímica S.C., Parque Tecnológico Querétaro s/n, Sanfandila, Pedro Escobedo 76703, Qro, Mexico. Electronic address: jmanriquez@cideteq.mx.
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Manríquez
D001205:Ascorbic Acid / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry; D003543:Cysteamine / Q000737:chemistry*; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles; D011073:Polyamines / Q000737:chemistry*; D014527:Uric Acid / Q000097:blood*
C531249:Poly(amidoamine); D011073:Polyamines; D014527:Uric Acid; D003543:Cysteamine; D002244:Carbon; D006046:Gold; D001205:Ascorbic Acid
Dendrimers; Gold nanoparticles; Human serum; Modified electrodes; Uric acid