The activity of N-acetyl-β-d-hexosaminidase A and B and β-glucuronidase in nasal polyps and hypertrophic nasal concha.
Nasal polyps and hypertrophic lower nasal conchae are common disorders of nasal cavity. The majority of etiopathogenetic theories indicate inflammatory background of polyps and hypertrophic concha. N-acetyl-β-D-hexosaminidase and β-glucuronidase are lysosomal exoglycosidases revealing accelerated activity in inflammatory processes.


OBJECTIVE
The aim of the study was to evaluate the catabolism of glycoconjugates in nasal polyps and hypertrophic nasal concha basing on the activity of N-acetyl-β-D-hexosaminidase (HEX) and β-glucuronidase (GLU).


METHODS
Material consisted of nasal polyps taken from 40 patients during polypectomy in patients with chronic rhinosinusitis with nasal polyps (CRSwNP) and hypertrophic lower nasal conchae taken from 20 patients during mucotomy. The activity of HEX, HEX A, HEX B and GLU in supernatant of homogenates of nasal polyps and hypertrophic lower nasal concha tissues has been estimated using colorimetric method.


RESULTS
Statistically significant decrease has been observed in concentration of the activity (per 1mg of tissue) of HEX (p<0.05), HEX B (p<0.001) and specific activity (per 1mg of protein) of HEX B (p<0.001) in nasal polyps tissue in comparison to hypertrophic lower nasal conchae tissue.


CONCLUSIONS
Decrease in the activity and specific activity concentration of the majority of examined lysosomal exoglycosidases (increasing in inflammations) in comparison to hypertrophic lower nasal conchae suggests electrolytes disorders and questions the inflammatory background of nasal polyps.
Medical Institute, College of Computer Science and Business Administration, Łomża, Poland. Electronic address: schojnowska@pwsip.edu.pl.
Sylwia
S
Chojnowska
Department of Surgical Nursing, Medical University of Bialystok, Poland.
Alina
A
Minarowska
Department of Psychiatry, Medical University of Białystok, Poland.
Napoleon
N
Waszkiewicz
Department of Biochemistry, Radioimmunology and Experimental Medicine, The Children's Memorial Health Institute, Warsaw, Poland.
Alina
A
Kępka
Department of Paediatric Radiology, Medical University of Białystok, Poland.
Beata
B
Zalewska-Szajda
Department of Paediatric Radiology, Medical University of Białystok, Poland.
Elżbieta
E
Gościk
Department of Allergology and Internal Medicine, Medical University of Białystok, Poland.
Krzysztof
K
Kowal
Department of Otolaryngology, Medical University of Białystok, Poland.
Ewa
E
Olszewska
Department of Emergency Medicine and Disasters, Medical University of Białystok, Poland.
Emilia
E
Konarzewska-Duchnowska
Department of Lung Diseases and Tuberculosis, Medical University of Bialystok, Poland.
Łukasz
Ł
Minarowski
Medical College of the Universal Education Society, Łomża, Poland.
Krzysztof
K
Zwierz
Department of Emergency Medicine and Disasters, Medical University of Białystok, Poland.
Jerzy Robert
JR
Ładny
Department of Emergency Medicine and Disasters, Medical University of Białystok, Poland.
Sławomir Dariusz
SD
Szajda
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D005966:Glucuronidase / Q000378:metabolism*; D054818:Hexosaminidase A / Q000378:metabolism*; D054819:Hexosaminidase B / Q000378:metabolism*; D006801:Humans; D006984:Hypertrophy / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000201:enzymology*; D014420:Turbinates / Q000201:enzymology* / Q000473:pathology; D055815:Young Adult
D005966:Glucuronidase; D054818:Hexosaminidase A; D054819:Hexosaminidase B
Hypertrophy of lower nasal concha; Isoensyme A of N-acetyl-β-d-hexosaminidase (HEX A); Isoensyme B of N-acetyl-β-d-hexosaminidase (HEX B); N-acetyl-β-d-hexosaminidase (HEX); Nasal polyps; β-Glucuronidase (GLU)