Pleural mesothelium lubrication after phospholipase treatment.
Coefficient of kinetic friction (μ) of rabbit pleural mesothelium increased after short treatment of specimens with phospholipase C. This increase was removed by addition of a solution with hyaluronan or sialomucin, as previously shown in post-blotting Ringer or after short pronase treatment. After phospholipase μ decreased with increase in sliding velocity, but at highest velocity it was still greater than control; this difference was removed by addition of hyaluronan or sialomucin, as in post-blotting Ringer or after short pronase treatment. Hyaluronan placed on specimen before phospholipase treatment reduced increase in μ by protecting phospholipids from enzyme, as shown by others for alveolar and synovial phospholipids. Samples of parietal pleura stained with silver nitrate showed that mesothelial cells were not disrupted by short phospholipase treatment. Instead, they were disrupted if this treatment was preceded by a short pronase treatment; but even after this disruption addition of hyaluronan or sialomucin brought μ back to control.
Dipartimento di Fisiopatologia e dei Trapianti, Sezione di Fisiologia Umana, Università degli Studi di Milano, Milan, Italy.
Francesca
F
Bodega
Dipartimento di Fisiopatologia e dei Trapianti, Sezione di Fisiologia Umana, Università degli Studi di Milano, Milan, Italy.
Chiara
C
Sironi
Dipartimento di Fisiopatologia e dei Trapianti, Sezione di Fisiologia Umana, Università degli Studi di Milano, Milan, Italy.
Cristina
C
Porta
Dipartimento di Fisiopatologia e dei Trapianti, Sezione di Fisiologia Umana, Università degli Studi di Milano, Milan, Italy.
Luciano
L
Zocchi
Dipartimento di Fisiopatologia e dei Trapianti, Sezione di Fisiologia Umana, Università degli Studi di Milano, Milan, Italy. Electronic address: emilio.agostoni@unimi.it.
Emilio
E
Agostoni
D000818:Animals; D003964:Diaphragm / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D004848:Epithelium / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D017276:Friction / Q000187:drug effects* / Q000502:physiology*; D006820:Hyaluronic Acid / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D010994:Pleura / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology*; D011817:Rabbits; D052116:Sialomucins / Q000494:pharmacology; D010738:Type C Phospholipases / Q000378:metabolism*
D052116:Sialomucins; D006820:Hyaluronic Acid; D010738:Type C Phospholipases
Hyaluronan; Kinetic friction; Phospholipase; Pleural mesothelium; Sialomucin; Sliding velocity