Short communication: validation of somatic cell score-associated loci identified in a genome-wide association study in German Holstein cattle.
Recently, we identified 6 genomic loci affecting daughter yield deviations (DYD) for somatic cell score (SCS) in a genome-wide association study (GWAS) performed with German Holstein bulls. In the current study, we tested if these loci were associated with SCS in cows using their own performance data. The study was performed with 1,412 German Holstein cows, of which 483 were daughters of 71 bulls that had been used in the GWAS. We tested 10 single nucleotide polymorphisms (SNP) representing 6 genomic regions that were associated with DYD for SCS in bulls. All tested SNP were significant in cows. Seven of them, located on Bos taurus autosomes (BTA) 6, 13, and 19, had the same direction of effect as those previously reported in the bull population. The most significant associations were detected on BTA6 and BTA19, accounting for 1.8% of the total genetic variance. The major allele of the 2 SNP on BTA6 and the minor allele of the 2 SNP on BTA19 were favorable for lower SCS. The differences between the homozygous genotype classes were up to 15,000 cells/mL. The verification of SNP associated with SCS in this study provides further evidence for the functional role of the linked genomic regions for immune response and contributes to identification of causative mutations. In particular, SNP with minor frequency of the favorable allele possess high potential to reduce SCS in German Holstein cattle by selection.
Breeding Biology and Molecular Genetics, Department for Crop and Animal Sciences, Humboldt-Universität zu Berlin, Invalidenstraße 42, 10115 Berlin, Germany.
Hamdy
H
Abdel-Shafy
Breeding Biology and Molecular Genetics, Department for Crop and Animal Sciences, Humboldt-Universität zu Berlin, Invalidenstraße 42, 10115 Berlin, Germany.
Ralf H
RH
Bortfeldt
Breeding Biology and Molecular Genetics, Department for Crop and Animal Sciences, Humboldt-Universität zu Berlin, Invalidenstraße 42, 10115 Berlin, Germany.
Monika
M
Reissmann
Breeding Biology and Molecular Genetics, Department for Crop and Animal Sciences, Humboldt-Universität zu Berlin, Invalidenstraße 42, 10115 Berlin, Germany. Electronic address: gudrun.brockmann@agrar.hu-berlin.de.
Gudrun A
GA
Brockmann
D000822:Animal Husbandry; D000818:Animals; D001947:Breeding; D002417:Cattle / Q000235:genetics*; D017931:DNA Primers; D003612:Dairying; D005260:Female; D055106:Genome-Wide Association Study / Q000662:veterinary*; D005858:Germany; D008297:Male; D008892:Milk / Q000166:cytology*; D020641:Polymorphism, Single Nucleotide
D017931:DNA Primers
candidate gene; mastitis resistance; single nucleotide polymorphism; validation study