Modification of catalase and MAPK in Vicia faba cultivated in soil with high natural radioactivity and treated with a static magnetic field.
The effects of a static magnetic field (SMF) and high natural radioactivity (HR) on catalase and MAPK genes in Vicia faba were investigated. Soil samples with high natural radioactivity were collected from Ramsar in north Iran where the annual radiation absorbed dose from background radiation is higher than 20mSv/year. The specific activity of the radionuclides of (232)Th, (236)Ra, and (40)K was measured using gamma spectrometry. The seeds were planted either in the soil with high natural radioactivity or in the control soils and were then exposed to a SMF of 30mT for 8 days; 8h/day. Levels of expression of catalase and MAPK genes, catalase activity and H2O2 content were evaluated. The results demonstrated significant differences in the expression of catalase and MAPK genes in SMF- and HR-treated plants compared to the controls. An increase in catalase activity was accompanied by increased expression of its gene and accumulation of H2O2. Relative expression of the MAPK gene in treated plants, however, was lower than those of the controls. The results suggest that the response of V. faba plants to SMF and HR may be mediated by modification of catalase and MAPK.
Department of Biophysics, Faculty of Biological Science, Tarbiat Modares University (TMU), POB 14115-154, Tehran, Iran.
Nazanin
N
Haghighat
Department of Biophysics, Faculty of Biological Science, Tarbiat Modares University (TMU), POB 14115-154, Tehran, Iran. Electronic address: parviz@modares.ac.ir.
Parviz
P
Abdolmaleki
Department of Plant Biology, Faculty of Biological Science, Tarbiat Modares University (TMU), POB 14115-154, Tehran, Iran.
Faezeh
F
Ghanati
Department of Genetics, Faculty of Biological Science, Tarbiat Modares University (TMU), POB 14115-154, Tehran, Iran.
Mehrdad
M
Behmanesh
Department of Plant Biology, Faculty of Biological Science, Tarbiat Modares University (TMU), POB 14115-154, Tehran, Iran.
Atefeh
A
Payez
D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism* / Q000528:radiation effects; D007492:Iran; D060526:Magnetic Fields; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011197:Potassium Radioisotopes / Q000633:toxicity; D011883:Radium / Q000633:toxicity; D012990:Soil Pollutants, Radioactive / Q000633:toxicity*; D013051:Spectrometry, Gamma; D013910:Thorium / Q000633:toxicity; D031307:Vicia faba / Q000201:enzymology / Q000235:genetics* / Q000528:radiation effects*
D010940:Plant Proteins; D011197:Potassium Radioisotopes; D012990:Soil Pollutants, Radioactive; D013910:Thorium; D006861:Hydrogen Peroxide; D002374:Catalase; D020928:Mitogen-Activated Protein Kinases; D011883:Radium
Catalase; High natural radioactivity; MAPK; Static magnetic field; Vicia faba