[Expression of SSB following early myocardial ischemia in rats].
OBJECTIVE
To investigate the expression of Sjögren's syndrome antigen B (SSB) gene and SSB protein in the early ischemic myocardium in rats.


METHODS
Adult healthy Sprague-Dawley rats were randomly divided into groups of operation [myocardial ischemia (MI) and non-ischemia (NI)], non-operation (NO) and sham-operation (SO) (n = 6 for MI and NI; n = 4 for NO and SO). According to time of ischemia, it was then divided into groups of 0 min, 15 min, 30 min, 60 min, 120 min, and 240 min. The expression of SSB gene in the myocardium was examined by real-time PCR, and the expression of SSB protein was examined by Western blot and immunofluorescence staining.


RESULTS
The expressions of SSB gene was down-regulated at early stage of ischemia. There was significant difference between 0 min and 120 min at the level of expression of SSB gene in MI group, so did that between 120 min group and NO group (P < 0.05). The expression of SSB protein at 60 min after ischemia was significantly decreased compared with that in the group of 0 min (P < 0.05). The expression of SSB protein in NI groups was significantly higher than that in MI groups at the time of 60 min and 120 min after myocardial ischemia (P < 0.05). Additionally, the expression of SSB protein was mainly located in the myocardial nucleus, myocardial plasma, and plasma membrane of partial myocardiocytes according to the result of immunofluorescence staining.


CONCLUSIONS
SSB may participate in pathophysiologic regulation process in myocardial cells at the early stage of myocardial ischemia in rats.
Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Tian-Yi
TY
Chen
Laboratory of Molecular and Translational Medicine, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Lin-Bo
LB
Gao
Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Wei-Bo
WB
Liang
Laboratory of Molecular and Translational Medicine, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Bin
B
Zhou
Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Hui
H
Xue
Laboratory of Molecular and Translational Medicine, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Yan-Yun
YY
Wang
Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Yun
Y
Liu
Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Lu-Shun
LS
Zhang
Laboratory of Molecular and Translational Medicine, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Ya-Ping
YP
Song
Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Lin
L
Zhang
D000818:Animals; D001324:Autoantigens / Q000235:genetics / Q000378:metabolism*; D008297:Male; D017202:Myocardial Ischemia / Q000235:genetics* / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D000097585:SS-B Antigen
D001324:Autoantigens; D012261:Ribonucleoproteins; D000097585:SS-B Antigen