Inflammation-related cytokines of aqueous humor in acute primary angle-closure eyes.
OBJECTIVE
To measure levels of various inflammation-related cytokines in the aqueous humor of patients with acute primary angle-closure (APAC) and senile cataract.


METHODS
Aqueous humor samples were prospectively collected from 23 eyes (12 eyes with current APAC and 11 eyes with previous APAC) of 23 APAC patients and 15 eyes of 15 cataract patients. The levels of 15 inflammation-related cytokines in the aqueous humor of APAC and cataract subjects were measured by using the multiplex bead immunoassay technique. Data on patient demographics and preoperative intraocular pressure (IOP) were also collected for correlation analysis.


RESULTS
Compared with the group with previous APAC and the cataract group, the group with current APAC showed clear and significantly elevated concentrations of interleukin (IL)-6, IL-8, granulocyte colony-stimulating factor (G-CSF), monocyte chemotactic protein (MCP)-1, MCP-3, and vascular endothelial growth factor (VEGF) (all P < 0.0167). The group with previous APAC and the cataract group had similar levels of cytokines. Intraocular pressure was positively correlated with IL-8 (P = 0.001), G-CSF (P = 0.002), MCP-3 (P < 0.001), and VEGF (P < 0.001).


CONCLUSIONS
An early "acute inflammatory" condition occurs in eyes with current APAC. In addition to controlling IOP, anti-inflammatory treatments are necessary for eyes suffering from APAC.
Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun Yat-sen University, Guangzhou, People's Republic of China.
Wenbin
W
Huang
Shida
S
Chen
Xinbo
X
Gao
Min
M
Yang
Jing
J
Zhang
Xingyi
X
Li
Wei
W
Wang
Minwen
M
Zhou
Xinyu
X
Zhang
Xiulan
X
Zhang
D000208:Acute Disease; D000368:Aged; D001082:Aqueous Humor / Q000378:metabolism*; D002386:Cataract / Q000378:metabolism; D018932:Chemokine CCL2 / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D015812:Glaucoma, Angle-Closure / Q000378:metabolism*; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism; D006801:Humans; D007118:Immunoassay / Q000379:methods; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D007429:Intraocular Pressure; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
C111835:CCL2 protein, human; C508606:CXCL8 protein, human; D018932:Chemokine CCL2; D016207:Cytokines; C508600:IL6 protein, human; D015850:Interleukin-6; D016209:Interleukin-8; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D016179:Granulocyte Colony-Stimulating Factor
acute primary angle-closure; aqueous humor; cytokines; inflammation