The effect of diesel exhaust exposure on blood-brain barrier integrity and function in a murine model.
Epidemiological studies indicate that exposure to diesel exhaust (DE) is associated with vascular-based disorders. To investigate the effect of DE on blood-brain barrier (BBB) function and integrity, 8-week-old BALB/c mice were randomized to DE in a cyclical treatment regimen over a 2-week period. Functional integrity of BBB was determined by considering brain parenchymal abundance of IgG within the hippocampal formation and cortex at 6 h and 24 h intervals following final exposure treatment. Neurovascular inflammation was expressed as the abundance of glial fibrillar acidic protein. Two doses of DE were studied and compared to air-only treated mice. Mice exposed to DE had substantially greater abundance of parenchymal IgG compared to control mice not exposed to DE. Increased parenchymal glial fibrillar acidic protein at 24 h post-DE exposure suggested heightened neurovascular inflammation. Our findings are proof-of-concept that inhalation of DE can compromise BBB function and support the broader contention that DE exposure may contribute to neurovascular disease risk.
Curtin Health Innovation Research Institute, School of Public Health, Curtin University of Technology, GPO Box U1987, Perth, WA, 6845, Australia.
Sayeh
S
Heidari Nejad
Ryusuke
R
Takechi
Benjamin J
BJ
Mullins
Corey
C
Giles
Alexander N
AN
Larcombe
Dean
D
Bertolatti
Krassi
K
Rumchev
Satvinder
S
Dhaliwal
John
J
Mamo
D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D002196:Capillaries / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D002540:Cerebral Cortex / Q000098:blood supply / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D002560:Cerebrovascular Circulation / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis; D006624:Hippocampus / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D008807:Mice, Inbred BALB C; D052638:Particulate Matter / Q000633:toxicity*; D011897:Random Allocation; D001335:Vehicle Emissions / Q000633:toxicity*
D000393:Air Pollutants; D005904:Glial Fibrillary Acidic Protein; D007074:Immunoglobulin G; D052638:Particulate Matter; D001335:Vehicle Emissions
Blood-brain barrier; Diesel exhaust; Diesel exhaust particle; Neuroinflammation