[Effects of Trichoderma longbrachitum and Streptomyces jingyangensis combination on the growth and disease resistance of tobacco seedlings].
An agar plate antagonism experiment in combining with in vivo screening experiment was conducted to study the affinity and bacteriostasis spectrum of the combination of biocontrol agents Trichoderma longbrachitum and Streptomyces jingyangensis to Nicotiana tabacum seedlings, with the effects of each agent and their combination on the N. tabacum seedlings growth, induced resistance, and resistance to Phytophthora nicotianae analyzed. The two agents had no interactive inhibitory effect and showed higher affinity to N. tabacum, and the agents themselves as well as their metabolites had higher bacteriostasis activities and wider bacteriostasis spectrum to P. nicotiaonae, Pythium aphanidermatum, and Alternaria alternate in different habitats. The combination of the two agents affected the morphological characteristics of the seedlings underground and aboveground parts, promoted the growth of root, stem, and leaf, and increased the root volume, total surface area, length, and average diameter as well as the stem height and size and the leaf length, width, and biomass, with these promotion effects being superior than those of the single-agent treatment. The combination of the two agents also increased the activities of the defensive enzymes superoxide dismutase, catalase, phenylalanine ammonia lyase, and peroxidase in the seedlings root significantly, with the relative control efficiency against P. nicotianae reached 69.3%, as compared to the conventional treatment. This study showed that the combination of T. longbrachitum and S. jingyangensis was a compatible combination with higher affinity and efficiency. This combination showed a synergistic effect of the two agents in plant disease control and in promoting plant growth, being able to promote the tobacco seedlings growth and control the P. nicotianae effectively.
Ministry of Agriculture Key Laboratory of Tobacco Biology and Processing/Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, Shandong, China. zhangliangonly2008@163.com
Liang
L
Zhang
Ministry of Agriculture Key Laboratory of Tobacco Biology and Processing/Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, Shandong, China.
Hao-Bao
HB
Liu
Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Jin-Gang
JG
Gu
Ministry of Agriculture Key Laboratory of Tobacco Biology and Processing/Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, Shandong, China.
Yu-Feng
YF
Song
Ministry of Agriculture Key Laboratory of Tobacco Biology and Processing/Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, Shandong, China.
Lian-Hong
LH
Dong
Ministry of Agriculture Key Laboratory of Tobacco Biology and Processing/Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, Shandong, China.
Yu-Long
YL
Su
Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Shi-Gui
SG
Li
Sichuan Tobacco Monopoly Administration, Chengdu 610000, China.
Qiang
Q
Lei
Sichuan Tobacco Monopoly Administration, Chengdu 610000, China.
Ren-Jun
RJ
Wu
Sichuan Tobacco Monopoly Administration, Chengdu 610000, China.
Ying
Y
Yin
Sichuan Tobacco Monopoly Administration, Chengdu 610000, China.
Jian-Kang
JK
Qu
Liangshan Tobacco Co. Ltd., Xichang 615000, Sichuan, China.
Yong
Y
Wang
D010572:Pest Control, Biological / Q000379:methods*; D010935:Plant Diseases / Q000517:prevention & control; D036226:Seedlings / Q000254:growth & development / Q000382:microbiology; D013302:Streptomyces / Q000502:physiology*; D014026:Nicotiana / Q000254:growth & development* / Q000382:microbiology; D014242:Trichoderma / Q000502:physiology*