Autophagy induced by resveratrol suppresses α-MSH-induced melanogenesis.
Autophagy degrades cellular components and organelles through a cooperative process involving autophagosomes and lysosomes. Although autophagy is known to mainly regulate the turnover of cellular components, the role of autophagy in melanogenesis has not been well addressed. Here, we show that inhibition of autophagy suppresses the antimelanogenesis activity of resveratrol (RSV), a well-known antimelanogenic agent. RSV strongly increased autophagy in melanocytes. However, the depletion of ATG5 significantly suppressed RSV-mediated antimelanogenesis as well as RSV-induced autophagy in melanocytes. Moreover, suppression of ATG5 retrieved the RSV-mediated downregulation of tyrosinase and TRP1 in α-MSH-treated cells. Most importantly, electron microscopy analysis revealed that autophagosomes engulfed melanin or melanosomes after combined treatment of α-MSH and RSV. Taken together, these results suggest that RSV-mediated autophagy regulates melanogenesis.
Graduate School of East-West Medical Science, Kyung Hee University, Yongin-si, Korea.
Eun Sung
ES
Kim
Huikyoung
H
Chang
Hyunjung
H
Choi
Ji Hyun
JH
Shin
So Jung
SJ
Park
Yoon Kyung
YK
Jo
Eun Sun
ES
Choi
Seok Yun
SY
Baek
Byung-Gyu
BG
Kim
Jong Wook
JW
Chang
Jin Cheon
JC
Kim
Dong-Hyung
DH
Cho
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D001343:Autophagy / Q000187:drug effects*; D000071187:Autophagy-Related Protein 5; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000187:drug effects; D006801:Humans; D008543:Melanins / Q000096:biosynthesis*; D008544:Melanocytes / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D020460:Melanosomes / Q000648:ultrastructure; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism; D014442:Monophenol Monooxygenase / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D014357:Trypsin / Q000378:metabolism; D000521:alpha-MSH / Q000494:pharmacology*
C502778:ATG5 protein, human; D000972:Antineoplastic Agents, Phytogenic; D000071187:Autophagy-Related Protein 5; D008543:Melanins; D008869:Microtubule-Associated Proteins; D013267:Stilbenes; D000521:alpha-MSH; D014442:Monophenol Monooxygenase; C489303:PRSS1 protein, human; D014357:Trypsin; D000077185:Resveratrol
autophagy; melanocytes; melanogenesis; resveratrol