Glutamine synthetase I-deficiency in Mesorhizobium loti differentially affects nodule development and activity in Lotus japonicus.
In this study, we focused on the effect of glutamine synthetase (GSI) activity in Mesorhizobium loti on the symbiosis between the host plant, Lotus japonicus, and the bacteroids. We used a signature-tagged mutant of M. loti (STM30) with a transposon inserted into the GSI (mll0343) gene. The L. japonicus plants inoculated with STM30 had significantly more nodules, and the occurrence of senesced nodules was much higher than in plants inoculated with the wild-type. The acetylene reduction activity (ARA) per nodule inoculated with STM30 was lowered compared to the control. Also, the concentration of chlorophyll, glutamine, and asparagine in leaves of STM30-infected plants was found to be reduced. Taken together, these data demonstrate that a GSI deficiency in M. loti differentially affects legume-rhizobia symbiosis by modifying nodule development and metabolic processes.
Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa 761-0795, Japan; Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakorn Pathom 73140, Thailand.
Sirinapa
S
Chungopast
Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa 761-0795, Japan; Faculty of Agriculture, Chiang Mai University, 50200 Chiang Mai, Thailand.
Pilunthana
P
Thapanapongworakul
Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa 761-0795, Japan.
Hiroyuki
H
Matsuura
Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa 761-0795, Japan; Faculty of Biology, Hanoi National University of Education, 136 Xuan Thuy Road, Hanoi, Vietnam.
Tan
T
Van Dao
Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa 761-0795, Japan.
Toshimasa
T
Asahi
Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa 761-0795, Japan.
Kuninao
K
Tada
Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa 761-0795, Japan.
Shigeyuki
S
Tajima
Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa 761-0795, Japan. Electronic address: nomura@ag.kagawa-u.ac.jp.
Mika
M
Nomura
D005974:Glutamate-Ammonia Ligase / Q000172:deficiency / Q000235:genetics*; D000070116:Lotus / Q000378:metabolism* / Q000382:microbiology*; D059128:Mesorhizobium / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D009586:Nitrogen Fixation; D018517:Plant Roots / Q000378:metabolism / Q000382:microbiology; D060888:Real-Time Polymerase Chain Reaction; D013559:Symbiosis
C081173:glutamine synthetase I; D005974:Glutamate-Ammonia Ligase
Glutamine synthetase; Mesorhizobium loti; Nodule; Senescence