FRET-enabled biological characterization of polymeric micelles.
Translation of micelles from the laboratory to the clinic is limited by a poor understanding of their in vivo fate following administration. In this paper, we establish a robust approach to real-time monitoring of the in vivo stability of micelles using Förster Resonance Energy Transfer (FRET). This characterization method allows for exquisite insight into the fate of micellar constituents, affording the capabilities to rapidly and efficiently evaluate a library of synthetically derived micellar systems as new therapeutic platforms in vivo. FRET-enabled biological characterization further holds potential to tailor material systems being uniquely investigated across the delivery community towards the next generation of stable therapeutics for disease management.
David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, USA; Department of Chemical Engineering, Massachusetts Institute of Technology, USA.
Stephen W
SW
Morton
David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, USA.
Xiaoyong
X
Zhao
David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, USA.
Mohiuddin A
MA
Quadir
David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, USA; Department of Chemical Engineering, Massachusetts Institute of Technology, USA; Institute for Soldier Nanotechnologies, Massachusetts Institute of Technology, USA. Electronic address: http://web.mit.edu/hammond/lab/
Paula T
PT
Hammond
D000818:Animals; D005260:Female; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism; D008027:Light; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008823:Micelles; D011108:Polymers / Q000737:chemistry*; D012542:Scattering, Radiation; D014018:Tissue Distribution
D005456:Fluorescent Dyes; D008823:Micelles; D011108:Polymers
Delivery; FRET; In vivo stability; Micelle; Self-assembly