Preparation of graphene oxide-based surface plasmon resonance biosensor with Au bipyramid nanoparticles as sensitivity enhancer.
A sensitive and selective wavelength-modulation surface plasmon resonance (SPR) biosensor based on graphene oxide (GO) and Au bipyramids (AuBPs) is reported for determination of bovine IgM. GO sheets with lengths of 100-300 nm are synthesized and assembled on amine-modified Au film. The large surface area and abundant functional groups of GO allow the efficient immobilization of antibody. AuBPs are nanoparticles with a penta-twinned crystal structure, which have a sharp localized surface plasmon resonance (LSPR) band because of their high monodispersity. In the optimal conditions, the GO-based biosensor with AuBPs as sensitivity enhancers shows a satisfactory response to bovine IgM in the concentration range of 0.03-32 μg mL(-1). For contrast, traditional biosensor, GO-based biosensor and GO-based biosensor with Au nanorods (AuNRs) as sensitivity enhancers for antigen detection were also investigated. Consequently, the as-prepared GO sheets function as promising support for antibody and GO-based SPR biosensor using AuBPs as enhancers has the highest sensitivity among the four types of biosensors.
College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, China.
Jia
J
Zhang
College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, China.
Ying
Y
Sun
College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, China.
Qiong
Q
Wu
College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, China.
Yan
Y
Gao
College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, China.
Hua
H
Zhang
Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Yu
Y
Bai
College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, China. Electronic address: songdq@jlu.edu.cn.
Daqian
D
Song
D000818:Animals; D015374:Biosensing Techniques; D002417:Cattle; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D007075:Immunoglobulin M / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D010316:Particle Size; D020349:Surface Plasmon Resonance; D013499:Surface Properties
D007075:Immunoglobulin M; D010087:Oxides; D006046:Gold; D006108:Graphite
Au bipyramids, IgM; Graphene oxide; Surface plasmon resonance