Antinociceptive and anti-inflammatory effects of flavonoids PMT1 and PMT2 isolated from Piper montealegreanum Yuncker (Piperaceae) in mice.
In this study, we identified the antinociceptive and anti-inflammatory effects of two flavonoids (PMT1 and PMT2) from Piper montealegreanum. The antinociceptive effect was evaluated using the classical tests: acetic acid-induced writhing, formalin and hot plate test. PMT1 and PMT2 (0.1, 1, 30 and 100 μmol/kg, i.p.) reduced the writhings, with an ID50 of 0.58 and 0.44 μmol/kg, respectively. Moreover, these flavonoids (100 μmol/kg, i.p.) inhibited paw-licking time in the neurogenic phase of the formalin test, but only PMT2 was active in the inflammatory phase. However, PMT1 and PMT2 (100 μmol/kg, i.p.) did not increase the latency time of the animals in the hot plate. In order to evaluate the anti-inflammatory effect of these flavonoids, capsaicin-induced ear oedema was carried out. Both flavonoids (100 μmol/kg, i.p.) were active in this model. These results suggest that PMT1 and PMT2 have antinociceptive and anti-inflammatory activities.
a Laboratório de Farmacologia e Imunidade, Instituto de Ciências Biológicas e da Saúde, Universidade Federal de Alagoas , Av. Lourival Melo Mota, s/n, Cidade Universitária, Maceió , AL , CEP: 57072-900 Brazil.
Aline Cavalcanti
AC
de Queiroz
Harley da Silva
Hda S
Alves
Luiz Henrique Agra
LH
Cavalcante-Silva
Thays de Lima Matos Freire
Tde L
Dias
Mariana da Silva
Mda S
Santos
Gabriela Muniz de Albuquerque
GM
Melo
Eliane Aparecida
EA
Campesatto
Maria Célia de Oliveira
MC
Chaves
Magna Suzana
MS
Alexandre-Moreira
D000700:Analgesics / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002211:Capsaicin / Q000494:pharmacology*; D004195:Disease Models, Animal; D004487:Edema / Q000188:drug therapy; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005557:Formaldehyde; D008297:Male; D051379:Mice; D015394:Molecular Structure; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy; D010147:Pain Measurement; D031701:Piper / Q000737:chemistry*
D000700:Analgesics; D000893:Anti-Inflammatory Agents; D005419:Flavonoids; C000592723:PMT1 flavonoid; C000592724:PMT2 flavonoid; D005557:Formaldehyde; D002211:Capsaicin