Development of a new biosensor for determination of catalase activity.
Catalase is one of the major antioxidant enzymes that catalyzes the hydrolysis of H2O2. The aim of this study was to suggest a new method for the assay of catalase activity. For this purpose, an amperometric biosensor based on glucose oxidase for determination of catalase activity was developed. Immobilization of glucose oxidase was made by a cross-linking method with glutaraldehyde on a Clark-type electrode (dissolved oxygen probe). Optimization and characterization properties of the biosensor were studied and determination of catalase activity in defined conditions was investigated in artificial serum solution. The results were compared with a reference method.
a Chemistry Department, Science Faculty , Muğla Sıtkı Koçman University , Muğla , Turkey.
Mustafa
M
Teke
D000818:Animals; D015374:Biosensing Techniques; D002138:Calibration; D002374:Catalase / Q000097:blood*; D002417:Cattle; D003432:Cross-Linking Reagents / Q000737:chemistry; D055664:Electrochemical Techniques; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D002374:Catalase; D010100:Oxygen; D005976:Glutaral