A cost-effective approach for detection of toxigenic Clostridium difficile: toxigenic culture using ChromID Clostridium difficile agar.
We evaluated the performance and the cost of toxigenic culture using a commercial chromogenic medium (CDIF) for 538 stool specimens. Compared with real-time PCR, this method was found to detect an additional 9% of positive specimens and result in 61% reduction in material costs, with a trade-off increase in turnaround time of 1 day.
Department of Pathology, Princess Margaret Hospital, Hong Kong, China.
Shik
S
Luk
Wing Kin
WK
To
Tak Keung
TK
Ng
Wai Ting
WT
Hui
Wing Keung
WK
Lee
Florence
F
Lau
Almond Man Wai
AM
Ching
D000362:Agar; D001427:Bacterial Toxins / Q000032:analysis*; D001431:Bacteriological Techniques / Q000191:economics / Q000379:methods*; D002863:Chromogenic Compounds / Q000378:metabolism; D016360:Clostridioides difficile / Q000302:isolation & purification*; D003015:Clostridium Infections / Q000175:diagnosis* / Q000382:microbiology; D003362:Cost-Benefit Analysis; D003470:Culture Media / Q000737:chemistry* / Q000191:economics; D005243:Feces / Q000382:microbiology; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D001427:Bacterial Toxins; D002863:Chromogenic Compounds; D003470:Culture Media; D000362:Agar