Embryoids derived from isolated protoplasts of carrot.
Protoplasts isolated enzymatically from carrot root tissues developed into cell clusters in a liquid medium containing coconut milk and naphthaleneacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D). Cells of the resulting calluses differentiated into embryoids on an agar medium containing coconut milk or kinetin.
Institute for Agricultural Research, Tohoku University, Sendai, Japan.
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Kameya
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Uchimiya