Local and remote ischemic preconditioning protect against intestinal ischemic/reperfusion injury after supraceliac aortic clamping.
OBJECTIVE
This study tests the hypothesis that local or remote ischemic preconditioning may protect the intestinal mucosa against ischemia and reperfusion injuries resulting from temporary supraceliac aortic clamping.


METHODS
Twenty-eight Wistar rats were divided into four groups: the sham surgery group, the supraceliac aortic occlusion group, the local ischemic preconditioning prior to supraceliac aortic occlusion group, and the remote ischemic preconditioning prior to supraceliac aortic occlusion group. Tissue samples from the small bowel were used for quantitative morphometric analysis of mucosal injury, and blood samples were collected for laboratory analyses.


RESULTS
Supraceliac aortic occlusion decreased intestinal mucosal length by reducing villous height and elevated serum lactic dehydrogenase and lactate levels. Both local and remote ischemic preconditioning mitigated these histopathological and laboratory changes.


CONCLUSIONS
Both local and remote ischemic preconditioning protect intestinal mucosa against ischemia and reperfusion injury following supraceliac aortic clamping.
Vascular Surgery, Department of Surgery, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto AlegreRS, Brazil.
Nilon
N
Erling Junior
Department of Surgery, Faculdade de Medicina da Universidade de São Paulo, São PauloSP, Brazil.
Edna Frasson de Souza
EF
Montero
Institute of Heart, Faculdade de Medicina da Universidade de São Paulo, São PauloSP, Brazil.
Paulina
P
Sannomiya
Department of Surgery, Faculdade de Medicina da Universidade de São Paulo, São PauloSP, Brazil.
Luiz Francisco
LF
Poli-de-Figueiredo
D000818:Animals; D001012:Aorta, Abdominal / Q000601:surgery*; D062186:Arterial Pressure; D003250:Constriction; D007413:Intestinal Mucosa / Q000098:blood supply* / Q000473:pathology; D007421:Intestine, Small / Q000098:blood supply / Q000473:pathology; D019194:Ischemic Preconditioning / Q000379:methods*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D015203:Reproducibility of Results; D013997:Time Factors; D016896:Treatment Outcome