Comparative Proteomics of Sera From HCC Patients With Different Origins.
BACKGROUND
Hepatocellular carcinoma (HCC), a major fatal cancer worldwide, is induced by different etiological factors in the liver.


OBJECTIVE
To gain insight into serum protein profiling of HCC with different etiologies.


METHODS
We subjected the sera of HBV-HCC, HCV-HCC, non-B non-C-HCC patients, and healthy volunteers to two-dimensional gel electrophoresis (2-DE) and liquid chromatography tandem mass spectrometry (LC-MS/MS).


RESULTS
We found 30 differentially expressed protein spots (≥ 1.5 fold P < 0.05) between these two analyses; of them 17 protein spots corresponding to 8 proteins were identified by MS. Transthyretin, leucine rich α-2-glycoprotein, and ficolin 3 were differentially expressed between HBV-related HCC and non-B non-C-HCC sera. Moreover, haptoglobin α-2 isoforms were decreased in HCV-HCC compared to non-B non-CHCC.


CONCLUSIONS
Serum proteome analyses of HCC with different origins showed a differential protein pattern, presumably related to different hepatopathogenesis in liver induced by different agents. Further studies are required to clarify the importance of identified proteins for early diagnosis of HCC with different origins.
Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IR Iran.
Jamal
J
Sarvari
Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IR Iran.
Zahra
Z
Mojtahedi
Department of Biochemistry and Functional Proteomics, Yamaguchi University Graduate School of Medicine, Yamaguchi, Japan.
Yasuhiro
Y
Kuramitsu
Department of Internal Medicine, School of Medicine, Shiraz University of Medical sciences, Shiraz, IR Iran.
Mohammad Reza
MR
Fattahi
Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IR Iran.
Abbas
A
Ghaderi
Department of Biochemistry and Functional Proteomics, Yamaguchi University Graduate School of Medicine, Yamaguchi, Japan.
Kazuyuki
K
Nakamura
Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IR Iran.
Nasrollah
N
Erfani
Biological Markers; Carcinoma, Hepatocellular; Electrophoresis, Gel, Two-Dimensional; Hepatitis B Virus; Hepatitis C