Backbone and stereospecific (13)C methyl Ile (δ1), Leu and Val side-chain chemical shift assignments of Crc.
Carbon catabolite repression (CCR) allows bacteria to selectively assimilate a preferred compound among a mixture of several potential carbon sources, thus boosting growth and economizing the cost of adaptability to variable nutrients in the environment. The RNA-binding catabolite repression control (Crc) protein acts as a global post-transcriptional regulator of CCR in Pseudomonas species. Crc triggers repression by inhibiting the expression of genes involved in transport and catabolism of non-preferred substrates, thus indirectly favoring assimilation of preferred one. We report here a nearly complete backbone and stereospecific (13)C methyl side-chain chemical shift assignments of Ile (δ1), Leu and Val of Crc (~ 31 kDa) from Pseudomonas syringae Lz4W.
CSIR - Centre for Cellular and Molecular Biology, Council of Scientific and Industrial Research (CSIR), Uppal Road, Hyderabad, 500007, AP, India.
Rakhi
R
Sharma
Bhubanananda
B
Sahu
Malay K
MK
Ray
Mandar V
MV
Deshmukh
D001426:Bacterial Proteins / Q000737:chemistry*; D007532:Isoleucine; D007930:Leucine; D019906:Nuclear Magnetic Resonance, Biomolecular; D044224:Pseudomonas syringae; D012097:Repressor Proteins / Q000737:chemistry*; D013237:Stereoisomerism; D014633:Valine
D001426:Bacterial Proteins; D012097:Repressor Proteins; C101896:crc protein, Pseudomonas; D007532:Isoleucine; D007930:Leucine; D014633:Valine