Efficient Recovery of Lignocellulolytic Enzymes of Spent Mushroom Compost from Oyster Mushrooms, Pleurotus spp., and Potential Use in Dye Decolorization.
This study was conducted in order to perform efficient extraction of lignocellulolytic enzymes amylase (EC 3.2.1.1), cellulase (EC 3.2.1.4), laccase (EC 1.10.3.2), and xylanase (EC 3.2.1.8) from spent mushroom compost (SMC) of Pleurotus ostreatus, P. eryngii, and P. cornucopiae. Optimal enzyme recovery was achieved when SMCs were extracted with 50 mM sodium citrate (pH 4.5) buffer at 4℃ for 2 hr. Amylase, cellulase, and xylanase activities showed high values in extracts from P. ostreatus SMC, with 2.97 U/g, 1.67 U/g, and 91.56 U/g, respectively, whereas laccase activity and filter paper degradation ability were highest in extracts from P. eryngii SMC, with values of 9.01 U/g and 0.21 U/g, respectively. Enzymatic activities varied according to the SMCs released from different mushroom farms. The synthetic dyes remazol brilliant blue R and Congo red were decolorized completely by the SMC extract of P. eryngii within 120 min, and the decolorization ability of the extract was comparable to that of 0.3 U of commercial laccase. In addition, laccase activity of the SMC extract from P. eryngii was compared to that of commercial enzymes or its industrial application in decolorization.
Graduate School of Future Convergence Technology, Hankyong National University, Ansung 456-749, Korea.
Seon-Hwa
SH
Lim
Mushroom Research Institute, GARES, Gwangju 464-870, Korea.
Yun-Hae
YH
Lee
Graduate School of Future Convergence Technology, Hankyong National University, Ansung 456-749, Korea. ; JK BioTech Co. Ltd., Ansung 456-749, Korea. ; Institute of Genetic Engineering, Hankyong National University, Ansung 456-749, Korea.
Hee-Wan
HW
Kang
Activity; Decolorization; Lignocellulolytic enzymes; Pleurotus spp.; Spent mushroom compost