Optimizing bioluminescence imaging to study protozoan parasite infections.
Bioluminescence imaging is a non-invasive technique which can be used to monitor infections in real-time. However, its utility is restricted by difficulties in detecting pathogens in deep tissue. 'Red-shifted' luciferases, which emit light of longer wavelength than standard bioluminescence-generating proteins, greatly enhance sensitivity, and have wide applicability for studying parasite infections.
Department of Pathogen Molecular Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
Martin C
MC
Taylor
Department of Pathogen Molecular Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK. Electronic address: john.kelly@lshtm.ac.uk.
John M
JM
Kelly
D000818:Animals; D004195:Disease Models, Animal; D056890:Eukaryota / Q000378:metabolism; D008156:Luciferases / Q000378:metabolism; D049449:Luminescence; D010273:Parasitic Diseases, Animal / Q000469:parasitology* / Q000473:pathology*; D010274:Parasitology / Q000379:methods*
D008156:Luciferases
bioluminescence; in vivo imaging; red-shifted luciferase; trypanosomes