Puniceibacterium antarcticum gen. nov., sp. nov., isolated from seawater.
A Gram-reaction-negative, aerobic, non-flagellated, rod-shaped bacterium, designated strain SM1211T, was isolated from Antarctic seawater. The isolate grew at 4-35 °C and with 0-10% (w/v) NaCl. It could produce bacteriochlorophyll a, but did not reduce nitrate to nitrite or hydrolyse DNA. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain SM1211T constituted a distinct phylogenetic line within the family Rhodobacteraceae and was closely related to species in the genera Litorimicrobium, Leisingera, Seohaeicola and Phaeobacter with 95.1-96.0% similarities. The predominant cellular fatty acid was C18:1ω7c. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid and two unidentified phospholipids. The genomic DNA G+C content of strain SM1211T was 60.7 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic data obtained in this study, strain SM1211T is considered to represent a novel species in a new genus within the family Rhodobacteraceae, for which the name Puniceibacterium antarcticum gen. nov., sp. nov. is proposed. The type strain of Puniceibacterium antarcticum is SM1211T (=CCTCC AB 2013147T=KACC 16875T).
Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.|State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
Chang
C
Liu
Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.|State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
Xi-Ying
XY
Zhang
Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.|State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
Hai-Nan
HN
Su
Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.|State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
Ming-Yang
MY
Zhou
SOA Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, PR China.
Bo
B
Chen
Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.|State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
Hai
H
Li
Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.|State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
Xiu-Lan
XL
Chen
Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.|State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
Dian-Li
DL
Zhao
Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.|State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
Bai-Cheng
BC
Zhou
Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.|State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
Mei
M
Shi
Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.|State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
Yu-Zhong
YZ
Zhang
D000864:Antarctic Regions; D015373:Bacterial Typing Techniques; D025541:Bacteriochlorophyll A / Q000737:chemistry; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
D025541:Bacteriochlorophyll A; D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S