Determination of nucleotides in infant milk formulas using novel dendrimer ion-exchangers.
The main aim of the present study was to develop a method for the separation of 5'-monophosphate nucleotides with the use of ion chromatography. Novel dendrimeric stationary phases were used for this purpose. The effects exerted by the type of anion-exchanger support (silica or polymeric) and the number of stationary phase layers on nucleotide retention were studied. A silica-based dendrimeric anion-exchanger was most suitable for analyzing the studied compounds. An increase in the number of layers enhanced nucleotide retention inside the column. The separation efficiency of the studied compounds was tested at various concentrations of the mobile phase buffer. At higher phosphate buffer concentrations, nucleotide resolutions were achieved in 6min. Three commercially available infant milk formulas were analyzed to verify the applicability of the studied method. Solid phase extraction was used for sample cleanup and concentration. The limit of quantification of nucleotides was 0.40μg/ml, and the method was linear in the concentration range of 0.40-20.6μgml(-1.)
Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarin St., 87-100 Toruń, Poland. Electronic address: kowalska@chem.umk.pl.
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Studzińska
Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarin St., 87-100 Toruń, Poland.
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Rola
Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarin St., 87-100 Toruń, Poland.
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Buszewski
D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D050091:Dendrimers / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007223:Infant; D041943:Infant Formula / Q000737:chemistry*; D016014:Linear Models; D009711:Nucleotides / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D050091:Dendrimers; D009711:Nucleotides
Dendrimer packing materials; Infant milk; Ion chromatography; Nucleotides monophosphates; Stationary phase