Real-time monitoring of auxin vesicular exocytotic efflux from single plant protoplasts by amperometry at microelectrodes decorated with nanowires.
Recent biochemical results suggest that auxin (IAA) efflux is mediated by a vesicular cycling mechanism, but no direct detection of vesicular IAA release from single plant cells in real-time has been possible up to now. A TiC@C/Pt-QANFA micro-electrochemical sensor has been developed with high sensitivity in detection of IAA, and it allows real-time monitoring and quantification of the quantal release of auxin from single plant protoplast by exocytosis.
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 (China).
Jun-Tao
JT
Liu
Liang-Sheng
LS
Hu
Yan-Ling
YL
Liu
Rong-Sheng
RS
Chen
Zhi
Z
Cheng
Shi-Jing
SJ
Chen
Christian
C
Amatore
Wei-Hua
WH
Huang
Kai-Fu
KF
Huo
D002244:Carbon / Q000737:chemistry; D055664:Electrochemical Techniques; D005089:Exocytosis; D007210:Indoleacetic Acids / Q000032:analysis*; D008839:Microelectrodes; D053770:Nanowires / Q000737:chemistry*; D010316:Particle Size; D010944:Plants / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry; D011522:Protons; D013499:Surface Properties; D013997:Time Factors; D014025:Titanium / Q000737:chemistry
D007210:Indoleacetic Acids; D011522:Protons; D010984:Platinum; D002244:Carbon; D014025:Titanium
amperometric monitoring; auxin efflux; electrochemical sensors; exocytosis; single plant cells