Methylation status of immune response genes promoters in cell-free DNA differs in hemodialyzed patients with diabetic nephropathy according to the intensity of anemia therapy.
BACKGROUND
Anemia is a major complication of end-stage renal disease. Hemodialysis itself is regarded as a stimulus activating inflammation. Pro-inflammatory cytokines are able to suppress erythropoiesis. In this pilot study, we analyzed the changes in methylation status of promoters of immune response genes in cell-free DNA to detect the differences between diabetic subjects (n = 18) with different therapeutic doses of recombinant erythropoietin.


METHODS
The extent of promoter methylation of 24 genes in plasma cell-free DNA was examined before and after hemodialysis using EpiTect Methyl qPCR Array Inflammatory Response and Autoimmunity (Qiagen).


RESULTS
The patients with higher methylation status of gene sequences IL13RA1, IL15, EDG3 and INHA in interdialytic interval were significantly overrepresented in the group with none or mild anemia therapy.


CONCLUSIONS
The results are in agreement with the fact that IL13 and IL15 are known inhibitors of erythropoiesis and with considered immunomodulatory role of cell-free DNA.
Institute of Biology and Medical Genetics and Institute of Medical Biochemistry and Laboratory Medicine, Charles University in Prague, Prague, Czech Republic.
Marie
M
Korabecna
Eva
E
Pazourkova
Ales
A
Horinek
Magdalena
M
Mokrejsova
Vladimir
V
Tesar
D000740:Anemia / Q000188:drug therapy / Q000209:etiology; D016000:Cluster Analysis; D016207:Cytokines / Q000235:genetics; D019175:DNA Methylation; D003928:Diabetic Nephropathies / Q000150:complications / Q000235:genetics* / Q000276:immunology* / Q000628:therapy; D004921:Erythropoietin / Q000627:therapeutic use; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007109:Immunity / Q000235:genetics*; D008297:Male; D011401:Promoter Regions, Genetic; D006435:Renal Dialysis / Q000009:adverse effects
D016207:Cytokines; D004921:Erythropoietin