[Protective effect of NMDA receptor antagonist memantine on acute lung injury in mice].
OBJECTIVE
To investigate the protective effect of a non-specific NMDA receptor antagonist memantine on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice.


METHODS
Healthy male mice were divided into 4 groups: a normal group, a memantine group, an ALI group and a memantine+ALI group. The ALI group was induced by intraperitoneal injection of LPS (10 mg/kg). Memantine (10 mg/kg) was injected intraperitoneally before the injection of LPS to determine the effect of blockade of NMDA receptor in the memantine+ALI group. The lung wet/dry ratio was detected. HE staining was preformed to show the morphological changes in the lung tissue. Myeloperoxidase enzyme (MPO) activity and malondialdehyde (MDA) content in the lung tissue were detected. ELISA was used to detect the tumor necrosis factor-α (TNF-α) content and lactate dehydrogenase (LDH) activity in the bronchoalveolar lavage fluid (BALF).


RESULTS
Memantine pretreatment improved the LPS-induced ALI lung tissue morphological changes, reduced their lung wet/dry ratio, the levels of TNF-α and LDH activity in BALF, and also reduced the MPO and MDA content in the lung tissue.


CONCLUSIONS
Blockade of NMDA receptors can ameliorate LPS-induced mice ALI.
Department of Physiology, Xiangya School of Basic Medicine, Central South University, Changsha 410078; Yongzhou Vocational and Technical College, Yongzhou Hunan 425000, China).
Ling
L
Ma
Wei
W
Liu
Dandan
D
Feng
Jianzhong
J
Han
Yang
Y
Li
Qingmei
Q
Cheng
Shaojie
S
Yue
Ziqiang
Z
Luo
D055371:Acute Lung Injury / Q000188:drug therapy*; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008070:Lipopolysaccharides; D008168:Lung / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008559:Memantine / Q000494:pharmacology*; D051379:Mice; D009195:Peroxidase / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D008070:Lipopolysaccharides; D016194:Receptors, N-Methyl-D-Aspartate; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; D009195:Peroxidase; D008559:Memantine