A light-activated metal complex targets both DNA and RNA in a fluorescent in vitro transcription and translation assay.
A coupled in vitro transcription and translation (IVTT) assay that uses GFP as a fluorescent reporter allowed the potency of a light-activated cytotoxic ruthenium agent to be quantified. The compound inhibits the function of both DNA and mRNA only upon light activation. The IVTT functional assay provides estimates of potency that are consistent with cellular cytotoxicity values, in marked contrast to the values obtained from traditional DNA-damage assays.
Department of Chemistry, University of Kentucky, 505 Rose St., Lexington, KY 40506 (USA).
David K
DK
Heidary
Edith C
EC
Glazer
D002474:Cell-Free System; D056831:Coordination Complexes / Q000737:chemistry / Q000378:metabolism*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004249:DNA Damage; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*; D012428:Ruthenium / Q000737:chemistry*; D014158:Transcription, Genetic
D056831:Coordination Complexes; D012333:RNA, Messenger; D049452:Green Fluorescent Proteins; D012428:Ruthenium; D004247:DNA
cancer; cisplatin; drug discovery; high-throughput screening; ruthenium