Electrochemical monitoring of an important biomarker and target protein: VEGFR2 in cell lysates.
Vascular endothelial growth factor receptor 2 (VEGFR2) is a potential cell-type biomarker in clinical diagnoses. Besides, it's the target protein of many tyrosine kinase inhibitors and its expression significantly associates with clinical performance of these inhibitors. VEGFR2 detection provides an early warning for diseases and a basis for therapy and drug screening. Some methods have been developed for VEGFR2 determination. However, they are usually performed indirectly and complexly. Herein, an electrochemical biosensing platform for VEGFR2 analysis has been first proposed. It can detect the total concentrations of the VEGFR2 protein in cells lysates directly and can be used to monitor the changes of VEGFR2 expression levels induced by treatments of different inhibitors. Moreover, the inhibitor-VEGFR2 interactions are illuminated through theoretical simulation. The simulation results agree well with the experimental data, indicating the veracity of the proposed method. The electrochemical detection methodology for VEGFR2 would be promising in clinical diagnosis and drug screening.
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials and Jiangsu Key Laboratory of Biofunctional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, P. R. China.
Tianxiang
T
Wei
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials and Jiangsu Key Laboratory of Biofunctional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, P. R. China.
Wenwen
W
Tu
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials and Jiangsu Key Laboratory of Biofunctional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, P. R. China.
Bo
B
Zhao
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials and Jiangsu Key Laboratory of Biofunctional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, P. R. China.
Yaqian
Y
Lan
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials and Jiangsu Key Laboratory of Biofunctional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, P. R. China.
Jianchun
J
Bao
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials and Jiangsu Key Laboratory of Biofunctional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, P. R. China.
Zhihui
Z
Dai
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D042783:Endothelial Cells / Q000187:drug effects; D006108:Graphite / Q000737:chemistry; D007118:Immunoassay / Q000379:methods; D008253:Macaca mulatta; D009536:Niacinamide / Q000031:analogs & derivatives / Q000494:pharmacology; D010084:Oxidation-Reduction; D010640:Phenothiazines / Q000737:chemistry; D010671:Phenylurea Compounds / Q000494:pharmacology; D010880:Piperidines / Q000494:pharmacology; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011799:Quinazolines / Q000494:pharmacology; D012160:Retina / Q000166:cytology; D000077157:Sorafenib; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000378:metabolism
D010640:Phenothiazines; D010671:Phenylurea Compounds; D010880:Piperidines; D047428:Protein Kinase Inhibitors; D011799:Quinazolines; D009536:Niacinamide; D006108:Graphite; D048271:Chitosan; D000077157:Sorafenib; D040301:Vascular Endothelial Growth Factor Receptor-2; C009469:thionine; C452423:vandetanib