The effect of indole-3-acetic acid on coleoptile extension growth in the absence of protein synthesis.
In wheat coleoptile sections cycloheximide inhibited over 90% of (14)C-leucine incorporation into protein within 10 minutes of its application. Even after 2-hour pretreatments with cycloheximide, IAA stimulated extension, suggesting that its growth-promoting action did not directly involve protein synthesis.Kinetic experiments with cycloheximide indicate that incorporation of a structural factor (possibly protein from a previously synthesised pool) into cell walls might be the rate-limiting process affected by IAA.
Dept. of Biology, Queen Elizabeth College (University of London), London.
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