Epigenetic modifiers alter the secondary metabolite composition of a plant endophytic fungus, Pestalotiopsis crassiuscula obtained from the leaves of Fragaria chiloensis.
The addition of the DNA methyltransferase inhibitor 500 μM 5-azacytidine to the culture medium of a plant endophytic fungus, Pestalotiopsis crassiuscula, obtained from the leaves of Fragaria chiloensis, dramatically altered the profiles of its metabolites and resulted in the isolation of one new coumarin (1), along with six known compounds (2-7). HPLC profiles revealed that only compounds 3, 4, and 7 belonged to the new induced secondary metabolites. The structures of all isolated compounds were elucidated on the basis of extensive analysis of NMR spectra.
a College of Pharmaceutical Science, Hebei University , Baoding 071002 , China.
Xiao-Long
XL
Yang
Le
L
Huang
Xiao-Li
XL
Ruan
D000818:Animals; D002176:Candida albicans / Q000187:drug effects; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D015254:DNA Modification Methylases / Q000037:antagonists & inhibitors; D057890:Epigenomics; D031985:Fragaria / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D018515:Plant Leaves / Q000382:microbiology; D013098:Xylariales / Q000737:chemistry*
D003374:Coumarins; D015254:DNA Modification Methylases