The effects of cadmium-zinc interactions on biochemical responses in tobacco seedlings and adult plants.
The objective of the present study was to investigate the effects of cadmium-zinc (Cd-Zn) interactions on their uptake, oxidative damage of cell macromolecules (lipids, proteins, DNA) and activities of antioxidative enzymes in tobacco seedlings as well as roots and leaves of adult plants. Seedlings and plants were exposed to Cd (10 µM and 15 µM) and Zn (25 µM and 50 µM) as well as their combinations (10 µM or 15 µM Cd with either 25 µM or 50 µM Zn). Measurement of metal accumulation exhibited that Zn had mostly positive effect on Cd uptake in roots and seedlings, while Cd had antagonistic effect on Zn uptake in leaves and roots. According to examined oxidative stress parameters, in seedlings and roots individual Cd treatments induced oxidative damage, which was less prominent in combined treatments, indicating that the presence of Zn alleviates oxidative stress. However, DNA damage found in seedlings, and lower glutathione reductase (GR) and superoxide dismutase (SOD) activity recorded in both seedlings and roots, after individual Zn treatments, indicate that Zn accumulation could impose toxic effects. In leaves, oxidative stress was found after exposure to Cd either alone or in combination with Zn, thus implying that in this tissue Zn did not have alleviating effects. In conclusion, results obtained in different tobacco tissues suggest tissue-dependent Cd-Zn interactions, which resulted in activation of different mechanisms involved in the protection against metal stress.
Department of Botany, Faculty of Science, University of Zagreb, Zagreb, Croatia.
Mirta
M
Tkalec
Department of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia.
Petra Peharec
PP
Stefanić
Department of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia.
Petra
P
Cvjetko
Department of Ecology, Institute of Public Health "Dr. Andrija Štampar", Zagreb, Croatia.
Sandra
S
Sikić
Department of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia.
Mirjana
M
Pavlica
Department of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia.
Biljana
B
Balen
D000704:Analysis of Variance; D002104:Cadmium / Q000737:chemistry* / Q000493:pharmacokinetics; D020552:Comet Assay; D005980:Glutathione Reductase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D018515:Plant Leaves / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D036226:Seedlings / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D014026:Nicotiana / Q000378:metabolism*; D015032:Zinc / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology
D002104:Cadmium; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D015032:Zinc