Genomic analyses of sodium channel α-subunit genes from strains of melon thrips, Thrips palmi, with different sensitivities to cypermethrin.
We examined the genomic organization of the sodium channel α-subunit gene in two strains of melon thrips, Thrips palmi, having differing sensitivity to cypermethrin. The nucleotide sequences of the strains included 18 or 16 putative exons which covered the entire coding region of the gene producing 2039 amino acid residues. Deduced amino acid sequences of both strains showed 80% homology with those of Periplaneta americana and Cimex lectularius. Comparison of deduced amino acid sequences of both strains showed no consistent amino acid difference. In addition to the previously reported resistant amino acid (Ile) at the T929I site, both strains encoded another resistant amino acids at two positions which are involved in pyrethroid resistance in other arthropods. These amino acids might also involve in the basal levels of resistance to pyrethroids of both strains.
Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan.
Wen Xue
WX
Bao
Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan.
Yoko
Y
Kataoka
Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan.
Yoko
Y
Kohara
Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan. Electronic address: sonodas@rib.okayama-u.ac.jp.
Shoji
S
Sonoda
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D023281:Genomics; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007305:Insecticide Resistance; D007306:Insecticides / Q000494:pharmacology*; D008969:Molecular Sequence Data; D011722:Pyrethrins / Q000494:pharmacology*; D016415:Sequence Alignment; D015222:Sodium Channels / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D061149:Thysanoptera / Q000737:chemistry / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism
D019476:Insect Proteins; D007306:Insecticides; D011722:Pyrethrins; D015222:Sodium Channels
Pyrethroid; Resistance; Sodium channel; Thrips