Anticoagulant and Antiplatelet Activities of Artemisia princeps Pampanini and Its Bioactive Components.
Artemisia princeps Pampanini (AP) has been used as a traditional medicine in Korea, China and Japan and reported to exhibit various beneficial biological effects including anti-inflammatory, antioxidant, anti-atherogenic and lipid lowering activities; however, its antiplatelet and anticoagulant properties have not been studied. In the present study, we evaluated the effects of an ethanol extract of Artemisia princeps Pampanini (EAP) and its major flavonoids, eupatilin and jaceosidin, on platelet aggregation and coagulation. To determine the antiplatelet activity, arachidonic acid (AA)-, collagen- and ADP (adenosine diphosphate)-induced platelet aggregation were examined along with serotonin and thromboxane A2 (TXA2) generation in vitro. The anticoagulant activity was determined by monitoring the activated partial thromboplastin time (aPTT) and prothrombin time (PT) in vitro. The data showed that EAP and its major flavonoids, eupatilin and jaceosidin, significantly reduced AA-induced platelet aggregation and the generation of serotonin and TXA2, although no significant change in platelet aggregation induced by collagen and ADP was observed. Moreover, EAP significantly prolonged the PT and aPTT. The PT and/or aPTT were significantly increased in the presence of eupatilin and jaceosidin. Thus, these results suggest that EAP may have the potential to prevent or improve thrombosis by inhibiting platelet activation and blood coagulation.
Department of Food Science and Nutrition, Kyungpook National University, Daegu 702-701, Korea.
Ri
R
Ryu
Center for Food and Nutritional Genomics Research, Kyungpook National University, Daegu 702-701, Korea.
Un Ju
UJ
Jung
Foods R&D, CJ Cheil Jedang Corporation, Seoul 152-051, Korea.
Hye-Jin
HJ
Kim
College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Korea ; Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Daegu 702-701, Korea.
Wonhwa
W
Lee
College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Korea.
Jong-Sup
JS
Bae
Department of Genetic Engineering, Kyungpook National University, Daegu 702-701, Korea.
Yong Bok
YB
Park
Department of Food Science and Nutrition, Kyungpook National University, Daegu 702-701, Korea ; Center for Food and Nutritional Genomics Research, Kyungpook National University, Daegu 702-701, Korea.
Myung-Sook
MS
Choi
Artemisia princeps Pampanini; anticoagulation; antiplatelet; eupatilin; jaceosidin