MicroRNA‑34a/c function as tumor suppressors in Hep‑2 laryngeal carcinoma cells and may reduce GALNT7 expression.
A family of small non-coding RNAs, ~22 nt in length, known as microRNAs (miRNAs), regulating ~30% of all human gene expression, have been reported to be involved in the pathogenesis of a number of types of cancers, including laryngeal squamous cell carcinoma (LSCC). In the current study, miR-34a and miR-34c were observed to be downregulated in human LSCC tissues. Ectopic expression of miR-34a and miR-34c in Hep-2 cells significantly induced the cell proliferation and migration ability in vitro. UDP-N-acetyl-α-D-galactosamine:polypeptide-N-acetylgalactosaminyltransferase 7 (GALNT7), whose expression is negatively regulated by miR-34a and miR-34c in Hep-2 cells, is conﬁrmed to be a novel direct target gene of miR-34a and miR-34c. In conclusion, the current results suggest that miR-34a and miR-34c may function as tumor suppressors in LSCC through downregulation of GALNT7. The study of miR-34a, miR-34c and its novel target, GALNT7, may serve as novel potential makers for LSCC therapy.
Department of Otolaryngology, Head and Neck Surgery, The First People's Hospital of Jining, Shandong 272000, P.R. China.
Wei
W
Li
Department of Respiration, The First People's Hospital of Jining, Shandong 272000, P.R. China.
Huiping
H
Ma
Department of Otolaryngology, Head and Neck Surgery, Harbin Medical University Cancer Institute and Hospital, Heilongjiang 150081, P.R. China.
Ji
J
Sun
D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D007822:Laryngeal Neoplasms / Q000201:enzymology* / Q000235:genetics* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D017350:N-Acetylgalactosaminyltransferases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D000097763:Polypeptide N-acetylgalactosaminyltransferase
D035683:MicroRNAs; D017350:N-Acetylgalactosaminyltransferases; D012333:RNA, Messenger; D000097763:Polypeptide N-acetylgalactosaminyltransferase; C522425:MIRN34 microRNA, human