CXCL12-G801A polymorphism modulates risk of colorectal cancer in Taiwan.
BACKGROUND
The chemokine CXCL12, designated stromal cell-derived factor-1 (SDF-1), plays a significant role in many cancer metastases. Previous studies have shown that CXCL12-G801A, a single nucleotide polymorphism (SNP) in the 3' untranslated region, correlates with breast and lung cancer in Iran. The aim of this study was to evaluate the association of the gene variant CXCL12-G801A with colorectal cancer (CRC) in a Taiwanese cohort.


METHODS
In this study, we used a denaturing high performance liquid chromatography (DHPLC) method to analyze the frequencies of CXCL12-G801A polymorphic variants between CRC patients (n = 258) and healthy controls (n = 300) in Taiwan.


RESULTS
The SNP distribution was higher in CRC patients with TNM stage II (117/258) than healthy controls (52/300). We observed a significant increase in the G/A plus A/A genotype of the CXCL12-G801A polymorphism in CRC patients (45.35%) compared with healthy controls (17.33%). The analysis of allelic frequencies in both groups revealed that CRC patients have a higher frequency of A allele (23.45%) than healthy controls (8.67%). Furthermore, among older CRC patients, the frequency of the CXCL12-G801A genotype was significantly increased (p = 0.0148).


CONCLUSIONS
Our observations suggest that the CXCL12-G801A genotype may be associated with some clinical manifestations in CRC patients in Taiwan.
Department of Pathology and Laboratory, Medicine Kaohsiung Veterans General Hospital Tainan Branch, Tainan, Taiwan ; Department of Medical Laboratory and Biotechnology Science, Kaohsiung Medical University, Kaohsiung, Taiwan.
Ming-Der
MD
Shi
School of Nutrition, Chung Shan Medical University, Taichung, Taiwan ; Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan.
Jing-Hsien
JH
Chen
Department of Pathology and Laboratory, Medicine Kaohsiung Veterans General Hospital Tainan Branch, Tainan, Taiwan.
Hsin-Te
HT
Sung
Department of Optometry, Chung Hwa University of Medical Technology, Tainan, Taiwan.
Jung-Shin
JS
Lee
Department of Medical Laboratory and Biotechnology Science, Kaohsiung Medical University, Kaohsiung, Taiwan.
Li-Yu
LY
Tsai
Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan ; School of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung, Taiwan.
Hui-Hsuan
HH
Lin
chemokine CXCL12; colorectal cancer; genotype; single nucleotide polymorphism