Taraxacum mongolicum extract exhibits a protective effect on hepatocytes and an antiviral effect against hepatitis B virus in animal and human cells.
In order to validate the antiviral effect against hepatitis B virus (HBV) of Taraxacum mongolicum (T. mongolicum), the protective effect on hepatocytes, and antiviral properties against duck hepatitis B virus (DHBV) and HBV of T. mongolicum extract (TME) were evaluated in chemically-injured neonatal rat hepatocytes, DHBV-infected duck fetal hepatocytes and HBV-transfected HepG2.2.15 cells, respectively. The results demonstrated that TME at 50-100 µg/ml improved D-galactosamine (D-GalN), thioacetamide (TAA) and tert-butyl hydroperoxide (t-BHP)-injured rat hepatocytes, and produced protection rates of 42.2, 34.6 and 43.8% at 100 µg/ml, respectively. Furthermore, TME at 1-100 µg/ml markedly inhibited DHBV DNA replication. Additionally, TME at 25-100 µg/ml reduced HBsAg and HBeAg levels and produced inhibition rates of 91.39 and 91.72% at 100 µg/ml, respectively. TME markedly inhibited HBV DNA replication at 25-100 µg/ml. The results demonstrate the potent antiviral effect of T. mongolicum against HBV effect. The protective of TME effect on hepatocytes may be achieved by its ability to ameliorate oxidative stress. The antiviral properties of TME may contribute to blocking protein synthesis steps and DNA replication. Furthermore, major components of TME were quantificationally analyzed. These data provide scientific evidence supporting the traditional use of TME in the treatment of hepatitis.
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, Department of Pharmacy, College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang 310018, P.R. China.
Yuan-Yuan
YY
Jia
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, Department of Pharmacy, College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang 310018, P.R. China.
Rong-Fa
RF
Guan
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, Department of Pharmacy, College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang 310018, P.R. China.
Yi-Hang
YH
Wu
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, Department of Pharmacy, College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang 310018, P.R. China.
Xiao-Ping
XP
Yu
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang 310031, P.R. China.
Wen-Yan
WY
Lin
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, Department of Pharmacy, College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang 310018, P.R. China.
Yong-Yong
YY
Zhang
College of Public Health, Xinjiang Medical University, Ürümqi, Xinjiang 830011, P.R. China.
Tao
T
Liu
College of Public Health, Xinjiang Medical University, Ürümqi, Xinjiang 830011, P.R. China.
Jun
J
Zhao
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang 310031, P.R. China.
Shu-Yun
SY
Shi
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang 310031, P.R. China.
Yu
Y
Zhao
D000818:Animals; D000831:Animals, Newborn; D000998:Antiviral Agents / Q000494:pharmacology*; D002109:Caffeic Acids / Q000737:chemistry / Q000494:pharmacology; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004372:Ducks; D005688:Galactosamine; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology; D056945:Hep G2 Cells; D015611:Hepatitis B Virus, Duck / Q000187:drug effects / Q000502:physiology; D006515:Hepatitis B virus / Q000187:drug effects / Q000502:physiology*; D022781:Hepatocytes / Q000187:drug effects / Q000473:pathology / Q000821:virology*; D006801:Humans; D047311:Luteolin / Q000737:chemistry / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013853:Thioacetamide; D020122:tert-Butylhydroperoxide
D000998:Antiviral Agents; D002109:Caffeic Acids; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D010936:Plant Extracts; D020011:Protective Agents; D013853:Thioacetamide; D005688:Galactosamine; D020122:tert-Butylhydroperoxide; C066408:luteolin-7-glucoside; D047311:Luteolin; C040048:caffeic acid