Effect of Persimmon Leaf Extract on Phthalic Anhydride-induced Allergic Response in Mice.
The purpose of this study was to investigate the anti-allergy activities of persimmon leaf extract (PLE) on a phthalic anhydride (PA)-induced allergic mouse model. A human leukemic mast cell line (HMC-1) was used to examine the inhibitory activity of PLE on the histamine release by human leukemic mast cells. PLE inhibited histamine release from HMC-1 cells in response to cross-linkage of high-affinity IgE receptor-α (FcεRIα). Additionally, a PA-induced allergic mouse model was used to investigate the effects of PLE in vivo. Mice were orally administrated with or without PLE of single dose (250 mg/kg/day) for 31 days. Oral intake of PLE significantly inhibited passive cutaneous reactions. Oral administration of PLE to PA-induced allergic mice also led to a striking suppression of the development of contact dermatitis, ear swelling and lymph node weight. In addition, PA-specific IL-4 production of draining lymph node cells was markedly diminished by PLE oral administration, but not IFN-γ. Furthermore, PLE treatment suppressed PA-induced thymus and activation-regulated chemokine (CCL17) and cutaneous T cell-attracting chemokine (CCL27) expressions in ear tissues. Based on these results, we suggest that PLE may have therapeutic potential as an effective material for management of irritant contact dermatitis or related inflammatory diseases.
Jeonju University Atopy & Health Research Institute, Jeonbuk 560-759, Korea.
Ji Ye
JY
Mok
School of Alternative Medicine & Health Science, Jeonbuk 560-759, Korea.
In Hwa
IH
Jeon
Department of Radiological Science, College of Alternative Medicine, Jeonju University, Jeonbuk 560-759, Korea.
Jung-Keun
JK
Cho
School of Alternative Medicine & Health Science, Jeonbuk 560-759, Korea.
Ji Min
JM
Park
School of Alternative Medicine & Health Science, Jeonbuk 560-759, Korea.
Hyeon Soo
HS
Kim
School of Alternative Medicine & Health Science, Jeonbuk 560-759, Korea.
Hyun Ju
HJ
Kang
Moakgol Association, Jeonbuk 565-821, Korea.
Hyung Soon
HS
Kim
Jeonju University Atopy & Health Research Institute, Jeonbuk 560-759, Korea ; School of Alternative Medicine & Health Science, Jeonbuk 560-759, Korea.
Seon Il
SI
Jang
IgE; chemokines; contact dermatitis; persimmon leaf extract; phthalic anhydride