Analgesic and anti-inflammatory activities of the 2,8-dihydroxy-1,6-dimethoxyxanthone from Haploclathra paniculata (Mart) Benth (Guttiferae).
In the present study, the pharmacological effects of 2,8-dihydroxy-1,6-dimethoxyxanthone from the bark of Haploclathra paniculata were investigated in mice using in vivo inflammation and nociception models. Acetic acid-induced writhing, paw licking induced by formalin, hot plate, and carrageenan-induced paw edema tests were used to investigate the anti-inflammatory and antinociceptive activities of the xanthone compound. Xanthone, at both doses, inhibited abdominal writhing and the formalin test. At a dose of 20 mg/kg, the time of reaction to the hot plate increased, and significant effects were observed after 30, 60 and 90 min of treatment. At doses of 10 and 20 mg/kg p.o., the 2,8-dihydroxy-1,6-dimethoxyxanthone significantly reduced paw edema at 3 h after the stimulus. The tests also showed no acute toxicity of the xanthone compound in mice. 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability was also studied and confirmed the antioxidant activity of the xanthone. To propose the mechanism of action of anti-inflammatory activity of the xanthone, a molecular docking was performed using the isoenzymes cyclooxygenase 1 and 2 and the results indicate that the molecule is capable of inhibiting both the enzymes. Therefore, it can be concluded that 2,8-dihydroxy-1,6-dimethoxyxanthone from H. paniculata demonstrates analgesic, anti-inflammatory, and antioxidant activities.
1 Department of Food Sciences of Federal University of Lavras-UFLA , Lavras-MG, Brazil .
Maria Eliza Castro
ME
Moreira
Rosemary Gualberto Fonseca Alvarenga
RG
Pereira
Marcelo Jose
MJ
Dias Silva
Danielle Ferreira
DF
Dias
Vanessa Silva
VS
Gontijo
Alexandre
A
Giusti-Paiva
Marcia Paranho
MP
Veloso
Antônio Carlos
AC
Doriguetto
Tanus Jorge
TJ
Nagem
Marcelo Henrique
MH
dos Santos
D019342:Acetic Acid; D000700:Analgesics / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use; D001713:Biphenyl Compounds / Q000378:metabolism; D002351:Carrageenan; D028084:Clusiaceae / Q000737:chemistry*; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology / Q000627:therapeutic use; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D006358:Hot Temperature; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy*; D008297:Male; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy*; D010147:Pain Measurement; D008517:Phytotherapy; D010851:Picrates / Q000378:metabolism; D024301:Plant Bark; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D017208:Rats, Wistar; D044004:Xanthones / Q000494:pharmacology / Q000627:therapeutic use*
C551473:2,8-dihydroxy-1,6-dimethoxyxanthone; D000700:Analgesics; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D052246:Cyclooxygenase 2 Inhibitors; D010851:Picrates; D010936:Plant Extracts; D044004:Xanthones; D002351:Carrageenan; C004931:1,1-diphenyl-2-picrylhydrazyl; D011451:Prostaglandin-Endoperoxide Synthases; D019342:Acetic Acid
DPPH; analgesic effect; anti-inflammatory activity; edema; inflammation; oxygenated xanthone