Novel properties of antimicrobial peptide anoplin.
Antimicrobial decapeptide anoplin was tested for its antifungal activity against plant pathogen Leptosphaeria maculans and protection of Brassica napus plants from disease. To reveal the mode of action of the peptide, a natural form of anoplin amidated on C-terminus (ANP-NH2), and its carboxylated analog (ANP-OH) were used in the study. We demonstrated strong antifungal activity of anoplin in vitro regardless C-terminus modification. In addition we show that both ANP-NH2 and ANP-OH induce expression of defence genes in B. napus and protects plants from L. maculans infection. The results indicate that the amidation of anoplin is not essential for its antifungal and plant defence stimulating activities.
Institute of Experimental Botany, Czech Academy of Science, Rozvojová 313, 165 02 Prague 6, Czech Republic; Department of Biochemistry and Microbiology, Institute of Chemical Technology Prague, Technická 5, 165 21 Prague 6, Czech Republic.
Barbora
B
Jindřichová
Institute of Experimental Botany, Czech Academy of Science, Rozvojová 313, 165 02 Prague 6, Czech Republic. Electronic address: burketova@ueb.cas.cz.
Lenka
L
Burketová
Department of Biochemistry and Microbiology, Institute of Chemical Technology Prague, Technická 5, 165 21 Prague 6, Czech Republic.
Zuzana
Z
Novotná
D000577:Amides / Q000737:chemistry / Q000494:pharmacology; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000494:pharmacology*; D001203:Ascomycota / Q000187:drug effects*; D029688:Brassica napus / Q000235:genetics / Q000382:microbiology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control*; D014862:Wasp Venoms / Q000737:chemistry / Q000494:pharmacology*
D000577:Amides; D000935:Antifungal Agents; D023181:Antimicrobial Cationic Peptides; D014862:Wasp Venoms; C443041:anoplin
Anoplin; Antifungal; Antimicrobial peptide; Brassica napus; Leptosphaeria maculans