Fluorous enzymatic synthesis of phosphatidylinositides.
A fluorous tagging strategy coupled with enzymatic synthesis is introduced to efficiently synthesize multiple phosphatidylinositides, which are then directly immobilized on a fluorous polytetrafluoroethylene (PTFE) membrane to probe protein-lipid interactions.
Division of Chemical Biology and Medicinal Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. qszhang@unc.edu.
Weigang
W
Huang
Angela
A
Proctor
Christopher E
CE
Sims
Nancy L
NL
Allbritton
Qisheng
Q
Zhang
D000906:Antibodies / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D019869:Phosphatidylinositol 3-Kinases / Q000737:chemistry; D010716:Phosphatidylinositols / Q000138:chemical synthesis* / Q000737:chemistry; D011138:Polytetrafluoroethylene / Q000737:chemistry; D040081:Protein Array Analysis; D010738:Type C Phospholipases / Q000737:chemistry
D000906:Antibodies; D005456:Fluorescent Dyes; D010716:Phosphatidylinositols; D011138:Polytetrafluoroethylene; D019869:Phosphatidylinositol 3-Kinases; D010738:Type C Phospholipases