The genetic incorporation of thirteen novel non-canonical amino acids.
Thirteen novel non-canonical amino acids were synthesized and tested for suppression of an amber codon using a mutant pyrrolysyl-tRNA synthetase-tRNA(Pyl)(CUA) pair. Suppression was observed with varied efficiencies. One non-canonical amino acid in particular contains an azide that can be applied for site-selective protein labeling.
Department of Chemistry, Texas A&M University, College Station, TX 77843, USA. wliu@chem.tamu.edu.
Alfred
A
Tuley
Yane-Shih
YS
Wang
Xinqiang
X
Fang
Yadagiri
Y
Kurra
Yohannes H
YH
Rezenom
Wenshe R
WR
Liu
D000596:Amino Acids / Q000235:genetics*; D018388:Codon, Terminator; D015394:Molecular Structure; D010649:Phenylalanine / Q000235:genetics*
D000596:Amino Acids; D018388:Codon, Terminator; D010649:Phenylalanine