Detection of immunoglobulins and complement components in formalin fixed and paraffin embedded renal biopsy material by immunoflourescence technique.
BACKGROUND
The technique of direct immunoflourescence (IF) is essential in the accurate diagnosis of renal glomerular diseases. The optimal results are obtained when the procedure is done on fresh frozen tissue (IF-F). However, techniques are available for IF study on formalin fixed and paraffin embedded (FFPE) renal biopsy specimens with variable reported success rates.


OBJECTIVE
We evaluated three such techniques on FFPE tissue and compared the results with those obtained by IF-F from the same patients.


METHODS
Heat treatment with Tris buffer and citrate buffer, and pronase treatment of the FFPE material was carried out. Direct IF was done for renal panel immunoglobulins and complement components on all biopsies and the results were compared with the historical IF-F study.


RESULTS
When compared to the IF-F, the immunoflourescence staining on the paraffin sections was less sensitive and less intense in all immune complex-mediated renal diseases, but the diagnostic findings were detected in majority of the cases.


CONCLUSIONS
In conclusion, it is possible to establish the diagnosis in most cases of immune complex-mediated glomerular diseases with IF on paraffin embedded tissue specimens.
Histopathology Department, Sindh Institute of Urology and Transplantation, Karachi, Pakistan.
Muhammed
M
Mubarak
Histopathology Department, Sindh Institute of Urology and Transplantation, Karachi, Pakistan.
I
I
Kazi Javed
Histopathology Department, Sindh Institute of Urology and Transplantation, Karachi, Pakistan.
Umme
U
Kulsoom
Histopathology Department, Sindh Institute of Urology and Transplantation, Karachi, Pakistan.
Muhammed
M
Ishaque
Complement; Frozen tissue; Immunoflourescence; Immunoglobulins; Paraffin embedded; Renal biopsies