The role of viral and host microRNAs in the Aujeszky's disease virus during the infection process.
Porcine production is a primary market in the world economy. Controlling swine diseases in the farm is essential in order to achieve the sector necessities. Aujeszky's disease is a viral condition affecting pigs and is endemic in many countries of the world, causing important economic losses in the swine industry. microRNAs (miRNAs) are non-coding RNAs which modulates gene expression in animals, plants and viruses. With the aim of understanding miRNA roles during the Aujeszky's disease virus [ADV] (also known as suid herpesvirus type 1 [SuHV-1]) infection, the expression profiles of host and viral miRNAs were determined through deep sequencing in SuHV-1 infected porcine cell line (PK-15) and in an animal experimental SuHV-1 infection with virulent (NIA-3) and attenuated (Begonia) strains. In the in vivo approach miR-206, miR-133a, miR-133b and miR-378 presented differential expression between virus strains infection. In the in vitro approach, most miRNAs were down-regulated in infected groups. miR-92a and miR-92b-3p were up-regulated in Begonia infected samples. Functional analysis of all this over expressed miRNAs during the infection revealed their association in pathways related to viral infection processes and immune response. Furthermore, 8 viral miRNAs were detected by stem loop RT-qPCR in both in vitro and in vivo approaches, presenting a gene regulatory network affecting 59 viral genes. Most described viral miRNAs were related to Large Latency Transcript (LLT) and to viral transcription activators EP0 and IE180, and also to regulatory genes regarding their important roles in the host-pathogen interaction during viral infection.
Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona (UAB), Bellaterra, Barcelona, Spain ; Departament de Genètica Animal, Centre de Recerca en AgriGenòmica (CRAG), CSIC-IRTA-UAB-UB, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
Oriol
O
Timoneda
Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, Bellaterra, Cerdanyola del Vallès, Spain.
Fernando
F
Núñez-Hernández
Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona (UAB), Bellaterra, Barcelona, Spain ; Departament de Genètica Animal, Centre de Recerca en AgriGenòmica (CRAG), CSIC-IRTA-UAB-UB, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
Ingrid
I
Balcells
Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, Bellaterra, Cerdanyola del Vallès, Spain.
Marta
M
Muñoz
Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona (UAB), Bellaterra, Barcelona, Spain ; Departament de Genètica Animal, Centre de Recerca en AgriGenòmica (CRAG), CSIC-IRTA-UAB-UB, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
Anna
A
Castelló
Departament de Genètica Animal, Centre de Recerca en AgriGenòmica (CRAG), CSIC-IRTA-UAB-UB, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
Gonzalo
G
Vera
Centro Nacional de Sanidad Agropecuaria (CENSA), La Habana, Cuba.
Lester J
LJ
Pérez
Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona (UAB), Bellaterra, Barcelona, Spain.
Raquel
R
Egea
CRAG scientific services, Centre de Recerca en AgriGenòmica (CRAG), CSIC-IRTA-UAB-UB, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
Gisela
G
Mir
Departament de Genètica Animal, Centre de Recerca en AgriGenòmica (CRAG), CSIC-IRTA-UAB-UB, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
Sarai
S
Córdoba
Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, Bellaterra, Cerdanyola del Vallès, Spain ; Departament d'Agricultura, Ramaderia, Pesca, Alimentació i Medi Natural de la Generalitat de Catalunya (DAR), Barcelona, Spain.
Rosa
R
Rosell
Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, Bellaterra, Cerdanyola del Vallès, Spain ; Departament de Sanitat i Anatomia Animals, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
Joaquim
J
Segalés
Program Infection and Immunity, FISIB, Bunyola, Spain.
Anna
A
Tomàs
Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona (UAB), Bellaterra, Barcelona, Spain ; Departament de Genètica Animal, Centre de Recerca en AgriGenòmica (CRAG), CSIC-IRTA-UAB-UB, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
Armand
A
Sánchez
Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, Bellaterra, Cerdanyola del Vallès, Spain.
José I
JI
Núñez
D000818:Animals; D002460:Cell Line; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D005814:Genes, Viral; D011558:Herpesvirus 1, Suid / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D054884:Host-Pathogen Interactions; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009830:Olfactory Bulb / Q000473:pathology / Q000821:virology; D011557:Pseudorabies / Q000235:genetics* / Q000276:immunology / Q000821:virology; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D013552:Swine; D013553:Swine Diseases / Q000235:genetics* / Q000276:immunology / Q000821:virology; D012668:Trigeminal Ganglion / Q000473:pathology / Q000821:virology; D014774:Virulence
D035683:MicroRNAs; D012333:RNA, Messenger