High-pressure open-channel on-chip electroosmotic pump for nanoflow high performance liquid chromatography.
Here, we construct an open-channel on-chip electroosmotic pump capable of generating pressures up to ∼170 bar and flow rates up to ∼500 nL/min, adequate for high performance liquid chromatographic (HPLC) separations. A great feature of this pump is that a number of its basic pump units can be connected in series to enhance its pumping power; the output pressure is directly proportional to the number of pump units connected. This additive nature is excellent and useful, and no other pumps can work in this fashion. We demonstrate the feasibility of using this pump to perform nanoflow HPLC separations; tryptic digests of bovine serum albumin (BSA), transferrin factor (TF), and human immunoglobulins (IgG) are utilized as exemplary samples. We also compare the performance of our electroosmotic (EO)-driven HPLC with Agilent 1200 HPLC; comparable efficiencies, resolutions, and peak capacities are obtained. Since the pump is based on electroosmosis, it has no moving parts. The common material and process also allow this pump to be integrated with other microfabricated functional components. Development of this high-pressure on-chip pump will have a profound impact on the advancement of lab-on-a-chip devices.
Department of Chemistry and Biochemistry, University of Oklahoma , 101 Stephenson Parkway, Norman, Oklahoma 73019, United States.
Wei
W
Wang
Congying
C
Gu
Kyle B
KB
Lynch
Joann J
JJ
Lu
Zhengyu
Z
Zhang
Qiaosheng
Q
Pu
Shaorong
S
Liu
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D053841:Electroosmosis / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D056656:Lab-On-A-Chip Devices; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000032:analysis
D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine