MiR-21 is under control of STAT5 but is dispensable for mammary development and lactation.
Development of mammary alveolar epithelium during pregnancy is controlled by prolactin, through the transcription factors STAT5A/B that activate specific sets of target genes. Here we asked whether some of STAT5's functions are mediated by microRNAs. The miR-21 promoter sequence contains a bona-fide STAT5 binding site and miR-21 levels increased in HC11 mammary cells upon prolactin treatment. In vivo miR-21 was abundantly expressed in mammary epithelium at day 6 of pregnancy. Analysis of mice lacking miR-21 revealed that their mammary tissue developed normally during pregnancy and dams were able to nurse their pups. Our study demonstrated that although expression of miR-21 is under prolactin control through the transcription factors STAT5A/B its presence is dispensable for mammary development and lactation.
Laboratory of Genetics and Physiology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, United States of America.
Yonatan
Y
Feuermann
Laboratory of Genetics and Physiology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, United States of America.
Keunsoo
K
Kang
Animal Science Departments, The Volcani Center, The Ministry of Agriculture, Bet Dagan, Israel.
Avi
A
Shamay
Laboratory of Genetics and Physiology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, United States of America.
Gertraud W
GW
Robinson
Laboratory of Genetics and Physiology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, United States of America.
Lothar
L
Hennighausen
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D004847:Epithelial Cells / Q000502:physiology; D005260:Female; D007774:Lactation / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000166:cytology / Q000254:growth & development* / Q000502:physiology; D018345:Mice, Knockout; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D050799:STAT5 Transcription Factor / Q000502:physiology*; D015533:Transcriptional Activation; D059467:Transcriptome
C531950:MIRN21 microRNA, mouse; D035683:MicroRNAs; D050799:STAT5 Transcription Factor