Identification of differentially expressed genes in hepatopancreas of oriental river prawn, Macrobrachium nipponense exposed to environmental hypoxia.
Hypoxia represents a major physiological challenge for prawn culture, and the hepatopancreas plays an important role in these processes. Here, we applied high-throughput sequencing technology to detect the gene expression profile of the hepatopancreas in Macrobrachium nipponense in response to hypoxia for 3 h and hypoxia for 24 h. Gene expression profiling identified 1925 genes that were significantly up- or down-regulated by dissolved oxygen availability. Functional categorization of the differentially expressed genes revealed that oxygen transport, electron transport chain, reactive oxygen species generation/scavenging, and immune response were the differentially regulated processes occurring during environmental hypoxia. Finally, quantitative real-time polymerase chain reaction using six genes independently verified the tag-mapped results. Immunohistochemistry analysis revealed, for the first time, hemocyanin protein expression as significant hypoxia-specific signature in prawns,which opens the way for in depth molecular studies of hypoxia exposure. The analysis of changes in hepatic gene expression in oriental river prawn provides a preliminary basis for a better understanding of the molecular response to hypoxia exposures.
Shengming
S
Sun
Fujun
F
Xuan
Xianping
X
Ge
Hongtuo
H
Fu
Jian
J
Zhu
Shiyong
S
Zhang
D000818:Animals; D016000:Cluster Analysis; D004777:Environment; D006433:Hemocyanins / Q000235:genetics; D043143:Hepatopancreas / Q000378:metabolism / Q000502:physiology; D057166:High-Throughput Screening Assays / Q000379:methods; D000860:Hypoxia / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D033541:Palaemonidae / Q000235:genetics* / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics
D006433:Hemocyanins; D010100:Oxygen