Generation and characterization of regulatory dendritic cells derived from murine induced pluripotent stem cells.
Regulatory dendritic cells (DCregs) represent a potential therapeutic tool for assessing a variety of immune overreaction conditions; however, current approaches for generating DCregs for therapeutic purposes are limited. We attempted to generate and characterize DCregs from murine induced pluripotent stem (iPS) cells. The iPS cells co-cultured with OP9 cells displayed mesodermally differentiated flat colonies. GM-CSF drove most of the colonies exhibiting a differentiated morphology. Thereafter, cells became morphologically heterologous under the effects of TGF-β and IL-10. Most of the floating cells developed an irregular shape with areas of protrusion. The generated iPS-DCregs demonstrated high CD11b/c and low CD40, CD80, CD86 and MHC-II expressions with a high antigen uptake ability and poor T-cell stimulatory function. Importantly, iPS-DCregs showed immune responsiveness regulation effects both in vitro and in vivo and the ability to generate regulatory T-cells in vitro. Our result illustrates a feasible approach for generating functional DCregs from murine iPS cells.
1] Department of Dermatology, Huashan hospital, Fudan University, Shanghai, China [2] Division of Transplantation Immunology, National Research Institute for Child Health and Development, Tokyo, Japan.
Qi
Q
Zhang
1] Division of Transplantation Immunology, National Research Institute for Child Health and Development, Tokyo, Japan [2] AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan.
Masayuki
M
Fujino
Division of Transplantation Immunology, National Research Institute for Child Health and Development, Tokyo, Japan.
Shizue
S
Iwasaki
1] Division of Transplantation Immunology, National Research Institute for Child Health and Development, Tokyo, Japan [2] Hasumi International Research Foundation, Tokyo, Japan.
Hiroshi
H
Hirano
Division of Transplantation Immunology, National Research Institute for Child Health and Development, Tokyo, Japan.
Songjie
S
Cai
Division of Transplantation Immunology, National Research Institute for Child Health and Development, Tokyo, Japan.
Yuya
Y
Kitajima
Department of Dermatology, Huashan hospital, Fudan University, Shanghai, China.
Jinhua
J
Xu
1] Department of Dermatology, Huashan hospital, Fudan University, Shanghai, China [2] Division of Transplantation Immunology, National Research Institute for Child Health and Development, Tokyo, Japan.
Xiao-Kang
XK
Li
D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D018122:B7-1 Antigen / Q000096:biosynthesis; D051940:B7-2 Antigen / Q000096:biosynthesis; D039481:CD11b Antigen / Q000096:biosynthesis; D039521:CD11c Antigen / Q000096:biosynthesis; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D019013:CD40 Antigens / Q000096:biosynthesis; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology*; D002460:Cell Line; D049109:Cell Proliferation; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology*; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D016753:Interleukin-10 / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D018122:B7-1 Antigen; D051940:B7-2 Antigen; D039481:CD11b Antigen; D039521:CD11c Antigen; D019013:CD40 Antigens; C497965:Cd86 protein, mouse; C556062:IL10 protein, mouse; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D016178:Granulocyte-Macrophage Colony-Stimulating Factor