Isolation, polarization, and expansion of CD4⁺ helper T cell lines and clones using magnetic beads.
Autoreactive CD4⁺ helper T cells specific for a range of nucleoprotein-derived autoantigens are an important feature of systemic lupus erythematosus, driving B cell differentiation and autoantibody production and contributing to the inflammatory lesions caused by immune complex deposition. Several peptide epitopes from nucleoprotein antigens have been identified and offer a means selectively to manipulate T cell responses by skewing toward a profile of cytokines that is less pro-inflammatory. Antigen-specific T cell lines and clones can be useful in the study of helper T cell subsets because their life span is prolonged and many individual cells can be generated, allowing particular phenotypes to be studied in detail. Magnetic beads offer a robust and convenient method for the isolation, polarization, and expansion of T cells, which can be adapted for a broad range of applications.
Antibody and Vaccine Group, Cancer Sciences Unit, Faculty of Medicine, Tenovus Research Laboratory, Southampton General Hospital, University of Southampton, Hampshire, UK.
Lekh N
LN
Dahal
Robert N
RN
Barker
Frank J
FJ
Ward
D018929:Cell Culture Techniques; D002460:Cell Line; D002999:Clone Cells; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D010641:Phenotype; D037182:T-Cell Antigen Receptor Specificity / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology* / Q000276:immunology / Q000378:metabolism