Effect of fumarate reducing bacteria on in vitro rumen fermentation, methane mitigation and microbial diversity.
The metabolic pathways involved in hydrogen (H(2)) production, utilization and the activity of methanogens are the important factors that should be considered in controlling methane (CH(4)) emissions by ruminants. H(2) as one of the major substrate for CH(4) production is therefore should be controlled. One of the strategies on reducing CH(4) is through the use of hydrogenotrophic microorganisms such as fumarate reducing bacteria. This study determined the effect of fumarate reducing bacteria, Mitsuokella jalaludinii, supplementation on in vitro rumen fermentation, CH(4) production, diversity and quantity. M. jalaludinii significantly reduced CH(4) at 48 and 72 h of incubation and significantly increased succinate at 24 h. Although not significantly different, propionate was found to be highest in treatment containing M. jalaludinii at 12 and 48 h of incubation. These results suggest that supplementation of fumarate reducing bacteria to ruminal fermentation reduces CH(4) production and quantity, increases succinate and changes the rumen microbial diversity.
Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, College of Bio-industry Science, Sunchon National University, Suncheon, 540-742, Republic of Korea.
Lovelia
L
Mamuad
Seon Ho
SH
Kim
Chang Dae
CD
Jeong
Yeon Jae
YJ
Choi
Che Ok
CO
Jeon
Sang-Suk
SS
Lee
D000818:Animals; D001105:Archaea; D001419:Bacteria / Q000145:classification / Q000378:metabolism*; D002417:Cattle; D058645:Denaturing Gradient Gel Electrophoresis; D005285:Fermentation / Q000502:physiology*; D005650:Fumarates / Q000378:metabolism*; D008697:Methane / Q000378:metabolism*; D064307:Microbiota; D012417:Rumen / Q000382:microbiology*; D014678:Veillonella
D005650:Fumarates; D008697:Methane