Copper, aluminum, iron and calcium inhibit human acetylcholinesterase in vitro.
Acetylcholinesterase (AChE) is an important part of cholinergic nerves where it participates in termination of neurotransmission. AChE can be inhibited by e.g. some Alzheimer disease drugs, nerve agents, and secondary metabolites. In this work, metal salts aluminum chloride, calcium chloride, cupric chloride, ferric chloride, potassium chloride, magnesium chloride and sodium chloride were tested for their ability to inhibit AChE. Standard Ellman assay based on human recombinant AChE was done and inhibition was measured using Dixon plot. No inhibition was proved for sodium, potassium and magnesium ions. However, aluminum, cupric, ferric and calcium ions were able to inhibit AChE via noncompetitive mechanism of inhibition. Though the inhibition is much weaker when compared to e.g. drugs with noncompetitive mechanism of action, biological relevance of the findings can be anticipated.
Faculty of Military Health Sciences, University of Defence, Trebesska 1575, CZ-500 01 Hradec Kralove, Czech Republic; Karel English College in Brno, Sujanovo namesti 356/1, 60200 Brno, Czech Republic. Electronic address: miroslav.pohanka@gmail.com.
Miroslav
M
Pohanka
D000110:Acetylcholinesterase / Q000378:metabolism; D000535:Aluminum / Q000633:toxicity*; D002118:Calcium / Q000633:toxicity*; D002800:Cholinesterase Inhibitors / Q000633:toxicity*; D003300:Copper / Q000633:toxicity*; D057809:HEK293 Cells; D006801:Humans; D007501:Iron / Q000633:toxicity*
D002800:Cholinesterase Inhibitors; D003300:Copper; D000535:Aluminum; D007501:Iron; D000110:Acetylcholinesterase; D002118:Calcium
Acetylcholinesterase; Alzheimer disease; Cholinergic system; Cholinesterase; Heavy metal; Inhibition