Microbiological evaluation of different irrigation protocols on root canal disinfection in teeth with apical periodontitis: an in vivo study.
The present in vivo study was designed to evaluate the efficacy of apical negative pressure irrigation (ANP), passive ultrasonic irrigation (PUI) and positive pressure irrigation (PP) in the reduction of intracanal bacteria of dogs' teeth with pulp necrosis and apical periodontitis. Eighty root canals were randomly distributed into 3 experimental and 2 control groups according to the irrigation delivery system: group ANP (n=20), group PUI (n=20), group PP (n=20), group PC (positive control - sterile saline irrigation; n=10) and group NC (negative control - vital pulps not subjected to bacterial inoculation; n=10). The first sample (S1) was collected at baseline, and the second sample (S2) was collected after the disinfection protocols. All samples were seeded in culture media for anaerobic bacteria. CFU counts were analyzed statistically by the Kruskal-Wallis, Dwass-Steel-Critchlow-Fligner post-hoc and Chi-square followed by Tukey like multiple comparisons for proportions (α=0.05). All experimental groups were effective in reducing Gram-positive bacteria compared with PC (p<0.05). Regarding the reduction of Gram-negative bacteria, group ANP was significantly better than PP (p<0.05). No statistically significant difference could be found between PP and PUI (p>0.05). In dog's teeth with apical periodontitis, the use of ANP and PUI can be considered promising disinfection protocols as both delivery systems promoted a significant bacterial reduction.
Department of Endodontics and Pediatric Dentistry, School of Dentistry, University of Washington, SeattleWA, USA.
Nestor
N
Cohenca
Department of Pediatric Dentistry, Ribeirão Preto School of Dentistry, USP - University of São Paulo, Ribeirão PretoSP, Brazil.
Lea Assed Bezerra
LA
Silva
Department of Pediatric Dentistry, Ribeirão Preto School of Dentistry, USP - University of São Paulo, Ribeirão PretoSP, Brazil.
Raquel Assed Bezerra
RA
Silva
Department of Pediatric Dentistry, Ribeirão Preto School of Dentistry, USP - University of São Paulo, Ribeirão PretoSP, Brazil.
Paulo
P
Nelson-Filho
Department of Endodontics, Universidad del Pacífico, Asunción, Paraguay.
Carlos
C
Heilborn
Department of Restorative Dentistry, Ribeirão Preto School of Dentistry, USP - University of São Paulo, Ribeirão PretoSP, Brazil.
Evandro
E
Watanabe
Department of Pediatric Dentistry, Ribeirão Preto School of Dentistry, USP - University of São Paulo, Ribeirão PretoSP, Brazil.
Maria Conceição Pereira
MC
Saraiva
D004203:Disinfection; D006801:Humans; D010485:Periapical Periodontitis / Q000628:therapy*; D012390:Root Canal Therapy; D007507:Therapeutic Irrigation