OsPT2, a phosphate transporter, is involved in the active uptake of selenite in rice.
• Selenite is a predominant form of selenium (Se) available to plants, especially in anaerobic soils, but the molecular mechanism of selenite uptake by plants is not well understood. • ltn1, a rice mutant previously shown to have increased phosphate (Pi) uptake, was found to exhibit higher selenite uptake than the wild-type in both concentration- and time-dependent selenite uptake assays. Respiratory inhibitors significantly inhibited selenite uptake in the wildtype and the ltn1 mutant, indicating that selenite uptake was coupled with H(+) and energy-dependent. Selenite uptake was greatly enhanced under Pi-starvation conditions, suggesting that Pi transporters are involved in selenite uptake. • OsPT2, the most abundantly expressed Pi transporter in the roots, is also significantly up-regulated in ltn1 and dramatically induced by Pi starvation. OsPT2-overexpressing and knockdown plants displayed significantly increased and decreased rates of selenite uptake, respectively, suggesting that OsPT2 plays a crucial role in selenite uptake. Se content in rice grains also increased significantly in OsPT2-overexpressing plants. • These data strongly demonstrate that selenite and Pi share similar uptake mechanisms and that OsPT2 is involved in selenite uptake, which provides a potential strategy for breeding Se-enriched rice varieties.
Henan University of Science and Technology, Luoyang, 471003, China.
Lianhe
L
Zhang
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.|National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
Bin
B
Hu
Henan University of Science and Technology, Luoyang, 471003, China.
Wei
W
Li
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.|National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
Ronghui
R
Che
Henan University of Science and Technology, Luoyang, 471003, China.
Kun
K
Deng
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.|National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
Hua
H
Li
Henan University of Science and Technology, Luoyang, 471003, China.
Feiyan
F
Yu
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
Hongqing
H
Ling
Henan University of Science and Technology, Luoyang, 471003, China.
Youjun
Y
Li
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.|National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
Chengcai
C
Chu
D019297:2,4-Dinitrophenol / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000031:analogs & derivatives / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D006859:Hydrogen / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D012275:Oryza / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D028061:Phosphate Transport Proteins / Q000235:genetics / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D019441:Plant Epidermis / Q000166:cytology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020887:Selenious Acid / Q000378:metabolism*; D012643:Selenium / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism; D027981:Symporters / Q000378:metabolism; D013997:Time Factors
D028061:Phosphate Transport Proteins; D010710:Phosphates; D010940:Plant Proteins; D012333:RNA, Messenger; D027981:Symporters; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D013455:Sulfur; D006859:Hydrogen; D020887:Selenious Acid; D012643:Selenium; D019297:2,4-Dinitrophenol
molecular mechanism; phosphate transporter; rice (Oryza sativa); selenite uptake; selenium (Se)