Polyacrylamide hydrogel micropatterning.
This chapter describes the production of micropatterns of extracellular matrix proteins on a 2D flat polyacrylamide (PAA) gel. The technique is divided into two parts. First, micropatterns are produced on glass or directly on a photomask using deep UV. Then the micropatterns are transferred on acrylamide gel by polymerization of the gel directly on the template coverslip. This procedure is easy to perform and does not require any expensive equipment. It can be performed in no more than 2h once you get your hands on it. It combines the advantages of other existing techniques: good spatial resolution, suitable for very soft gel, no need for the use of chemical crosslinkers for attachment of the proteins to the acrylamide, no modification of the mechanical properties of the gel by the process, and suitable for multiple protein patterning. We also discuss the storage issues of such substrates and provide a brief review of other existing techniques for micropatterning on PAA.
Laboratoire de Physiologie Cellulaire & Végétale, CNRS, UMR 5168, Grenoble, France; LPCV, University of Grenoble Alpes, Grenoble, France; LPCV, DSV, CEA, iRTSV, Grenoble, France; LPCV, INRA, USC1359, Grenoble, France.
Timothée
T
Vignaud
Laboratoire de Physiologie Cellulaire & Végétale, CNRS, UMR 5168, Grenoble, France; LPCV, University of Grenoble Alpes, Grenoble, France; LPCV, DSV, CEA, iRTSV, Grenoble, France; LPCV, INRA, USC1359, Grenoble, France.
Hajer
H
Ennomani
Laboratoire de Physiologie Cellulaire & Végétale, CNRS, UMR 5168, Grenoble, France; LPCV, University of Grenoble Alpes, Grenoble, France; LPCV, DSV, CEA, iRTSV, Grenoble, France; LPCV, INRA, USC1359, Grenoble, France.
Manuel
M
Théry
D000180:Acrylic Resins / Q000737:chemistry*; D000268:Adhesiveness; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D055616:Microtechnology / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry; D011107:Polylysine / Q000737:chemistry; D014466:Ultraviolet Rays; D000099166:Polyacrylamides
D000180:Acrylic Resins; D016326:Extracellular Matrix Proteins; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols; D011107:Polylysine; D000099166:Polyacrylamides
Cell adhesion; Extracellular matrix; Hydrogel; Micropattern; Polyacrylamide; Rigidity; Traction forces