Positive control for cytotoxicity evaluation of dental vinyl polysiloxane impression materials using sodium lauryl sulfate.
OBJECTIVE
Vinyl polysiloxane (VPS) is elastomeric dental impression material which, despite having very few reports of adverse reactions, has shown high levels of cytotoxicity that is difficult to be interpreted without referencing to the positive control material. Therefore, in this study, positive control VPS was developed using sodium lauryl sulfate (SLS) for the reference of cytotoxicity test.


METHODS
The positive control VPS with SLS was formed with a different proportion of SLS (0, 1, 2, 4, 8 and 16 wt%) added to the base. The cytotoxicity test was then carried out using the extractions or dilutions of the extractions from each of the test samples using murine fibroblast cells (L929).


RESULTS
The final product of positive control VPS behaved similar to commercially available VPS; being initially liquid-like and then becoming rubber-like. Ion chromatography showed that the level of SLS released from the product increased as the proportion of added SLS increased, consequently resulting in an increased level of cytotoxicity. Also, the commercially available VPS was less cytotoxic than the positive control VPS with more or equal to 2 wt% of SLS. However, even the VPS with the highest SLS (16 wt%) did not cause oral mucosa irritation during the animal study.


CONCLUSIONS
The positive control VPS was successfully produced using SLS, which will be useful in terms of providing references during in vitro cytotoxicity testing.
Brain Korea 21 PLUS Project, Department and Research Institute of Dental Biomaterials and Bioengineering.
Jae-Sung
JS
Kwon
Sang-Bae
SB
Lee
Kwang-Mahn
KM
Kim
Kyoung-Nam
KN
Kim
D000818:Animals; D002460:Cell Line; D002852:Chromatography, Ion Exchange; D006224:Cricetinae; D003760:Dental Impression Materials / Q000032:analysis / Q000633:toxicity*; D005347:Fibroblasts / Q000187:drug effects; D008422:Materials Testing; D008647:Mesocricetus; D051379:Mice; D009061:Mouth Mucosa / Q000187:drug effects; D011145:Polyvinyls / Q000032:analysis / Q000633:toxicity*; D012833:Siloxanes / Q000032:analysis / Q000633:toxicity*; D012967:Sodium Dodecyl Sulfate / Q000032:analysis / Q000633:toxicity*; D013431:Sulfates / Q000032:analysis; D013501:Surface-Active Agents / Q000032:analysis / Q000633:toxicity*; D013997:Time Factors
D003760:Dental Impression Materials; D011145:Polyvinyls; D012833:Siloxanes; D013431:Sulfates; D013501:Surface-Active Agents; C034183:vinyl polysiloxane; D012967:Sodium Dodecyl Sulfate
biocompatibility; cytotoxicity; elastomeric impression materials; sodium lauryl sulfate; vinyl polysiloxane