Expression of OsMATE1 and OsMATE2 alters development, stress responses and pathogen susceptibility in Arabidopsis.
Multidrug and Toxic compound Extrusion proteins (MATE) are a group of secondary active transporters with ubiquitous occurrences in all domains of life. This is a newly characterized transporter family with limited functional knowledge in plants. In this study, we functionally characterised two members of rice MATE gene family, OsMATE1 and OsMATE2 through expression in heterologous system, Arabidopsis. Expression of OsMATEs in Arabidopsis altered growth and morphology of transgenic plants. Genome-wide expression analysis revealed modulation of genes involved in plant growth, development and biotic stress in transgenic lines. Transgenic plants displayed sensitivity for biotic and abiotic stresses. Elevated pathogen susceptibility of transgenic lines was correlated with reduced expressions of defence related genes. Promoter and cellular localization studies suggest that both MATEs express in developing and reproductive organs and are plasma-membrane localised. Our results reveal that OsMATE1 and OsMATE2 regulate plant growth and development as well as negatively affect disease resistance.
National Botanical Research Institute, Council of Scientific and Industrial Research (CSIR), Rana Pratap Marg, Lucknow-226 001, INDIA.
Manish
M
Tiwari
National Botanical Research Institute, Council of Scientific and Industrial Research (CSIR), Rana Pratap Marg, Lucknow-226 001, INDIA.
Deepika
D
Sharma
Department of Botany, University of Lucknow, Lucknow-226007, INDIA.
Munna
M
Singh
National Botanical Research Institute, Council of Scientific and Industrial Research (CSIR), Rana Pratap Marg, Lucknow-226 001, INDIA.
Rudra Deo
RD
Tripathi
National Botanical Research Institute, Council of Scientific and Industrial Research (CSIR), Rana Pratap Marg, Lucknow-226 001, INDIA.
Prabodh Kumar
PK
Trivedi
D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D001151:Arsenic / Q000494:pharmacology; D035264:Flowers / Q000254:growth & development; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D020022:Genetic Predisposition to Disease; D027701:Organic Cation Transport Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D012275:Oryza / Q000201:enzymology / Q000235:genetics; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000254:growth & development; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000254:growth & development; D036226:Seedlings / Q000254:growth & development
D027701:Organic Cation Transport Proteins; D010940:Plant Proteins; D001151:Arsenic