In situ protocol for the determination of dose-response effect of low-fluoride dentifrices on enamel remineralization.
No in situ protocol has assessed the dose-response effects of fluoride dentifrices involving low-fluoride formulations.


OBJECTIVE
To assess the ability of an in situ remineralization model in determining dose-response effects of dentifrices containing low fluoride concentrations ([F]) on bovine enamel.


METHODS
Volunteers wore palatal appliances containing demineralized enamel blocks and brushed their teeth and devices with the dentifrices supplied (double-blind, crossover protocol) separately for 3 and 7 days. Surface hardness (SH), integrated subsurface hardness (ΔKHN) and [F] in enamel were determined. Data were analyzed by ANOVA, Tukey's test and Pearson's correlation (p<0.05).


RESULTS
Dose-response relationships were verified between [F] in dentifrices and SH, ΔKHN and enamel [F]. Higher correlation coefficients between enamel [F] and SH and ΔKHN were obtained for the 3-day period. Significant differences in SH and ΔKHN were observed among all groups for the 3-day period, but not between 0-275, 275-550, and 550-1,100 µg F/g dentifrices for the 7-day period, nor between 3- and 7-day periods for the 1,100 µg F/g groups.


CONCLUSIONS
Considering that the peak remineralization capacity of the conventional dentifrice (1,100 µg F/g) was achieved in 3 days, this experimental period could be used in future studies assessing new dentifrice formulations, especially at low-fluoride concentrations.
Rebeca Lima
RL
Afonso
Juliano Pelim
JP
Pessan
Bruna Babler
BB
Igreja
Camila Fernandes
CF
Cantagallo
Marcelle
M
Danelon
Alberto Carlos Botazzo
AC
Delbem
D000328:Adult; D000704:Analysis of Variance; D000818:Animals; D002417:Cattle; D018592:Cross-Over Studies; D003743:Dental Enamel / Q000187:drug effects*; D003802:Dentifrices / Q000494:pharmacology* / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D004311:Double-Blind Method; D005260:Female; D005459:Fluorides / Q000494:pharmacology* / Q000627:therapeutic use; D006245:Hardness Tests; D006801:Humans; D008297:Male; D013499:Surface Properties / Q000187:drug effects; D013997:Time Factors; D017001:Tooth Demineralization / Q000188:drug therapy*; D014089:Tooth Remineralization / Q000379:methods*; D016896:Treatment Outcome; D055815:Young Adult
D003802:Dentifrices; D005459:Fluorides