[Identification and localisation of tryptophan synthetase in seeds of Juglans regia L].
Cell-free extracts from seeds of Juglans regia synthesize tryptophan from L-serine and indole. Tryptophan synthetase has maximal activity in the range between pH 7 and 8. The enzyme is associated with a particulate fraction (density 1,210 g/ml) which is separated from the mitochondria (density 1,191 g/ml) after isopycnic density centrifugation on a continuous sucrose gradient.
Botanisches Institut der Universität zu Köln, Gyrhofstraße 15, D-5000, Köln 41, Bundesrepublik Deutschland.
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