Sequential acid and enzymatic hydrolysis in situ and bioethanol production from Gracilaria biomass.
Gracilaria sp., a red alga, was used as a feedstock for the production of bioethanol. Saccharification of Gracilaria sp. by sequential acid and enzyme hydrolysis in situ produced a high quality hydrolysate that ensured its fermentability to produce ethanol. The optimal saccharification process resulted in total 11.85g/L (59.26%) of glucose and galactose, Saccharomyces cerevisiae Wu-Y2 showed a good performance on co-fermentability of glucose and galactose released in the hydrolysate from Gracilaria sp. The final ethanol concentrations of 4.72g/L (0.48g/g sugar consumed; 94% conversion efficiency) and the ethanol productivity 4.93g/L/d were achieved. 1g of dry Gracilaria can be converted to 0.236g (23.6%) of bioethanol via the processes developed. Efficient alcohol production by immobilized S. cerevisiae Wu-Y2 in batch and repeated batch fermentation was also demonstrated. The findings of this study revealed that Gracilaria sp. can be a potential feedstock in biorefinery for ethanol production.
Department of Bioindustry Technology, Da-Yeh University, Changhua, Taiwan.
Fang-Chen
FC
Wu
Department of Bioindustry Technology, Da-Yeh University, Changhua, Taiwan.
Jane-Yii
JY
Wu
Department of Environmental Engineering, Da-Yeh University, Changhua, Taiwan.
Yi-Jyun
YJ
Liao
Department of Environmental Engineering, Da-Yeh University, Changhua, Taiwan.
Man-Ying
MY
Wang
Department of Environmental Engineering, Da-Yeh University, Changhua, Taiwan. Electronic address: ils@mail.dyu.edu.tw.
Ing-Lung
IL
Shih
D056804:Biofuels; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism / Q000187:drug effects; D018914:Cells, Immobilized / Q000187:drug effects / Q000378:metabolism; D002480:Cellulase / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D044724:Gracilaria / Q000166:cytology / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis / Q000187:drug effects; D013464:Sulfuric Acids / Q000494:pharmacology*; D013997:Time Factors
D056804:Biofuels; D013464:Sulfuric Acids; D000431:Ethanol; D002480:Cellulase; C033158:sulfuric acid
Bioethanol; Enzymatic hydrolysis; Gracilaria; Saccharification; Saccharomyces cerevisiae