Nuclear receptor-mediated alleviation of alcoholic fatty liver by polyphenols contained in alcoholic beverages.
To elucidate the effect of the polyphenols contained in alcoholic beverages on the metabolic stress induced by ethanol consumption, four groups of mice were fed for five weeks on Lieber's diet with or without ethanol, with ethanol plus ellagic acid, and with ethanol plus trans-resveratrol. Alcoholic fatty liver was observed in the group fed the ethanol diet but not in those fed the ethanol plus polyphenol diets. Liver transcriptome analysis revealed that the addition of the polyphenols suppressed the expression of the genes related to cell stress that were up-regulated by ethanol alone. Conversely, the polyphenols up-regulated the genes involved in bile acid synthesis, unsaturated fatty acid elongation, and tetrahydrofolate synthesis that were down-regulated by ethanol alone. Because parts of these genes were known to be regulated by the constitutive androstane receptor (CAR), we performed the same experiment in the CAR-deficient mice. As a result, fatty liver was observed not only in the ethanol group but also with the ethanol plus polyphenol groups. In addition, there was no segregation of the gene expression profiles among these groups. These results provide a molecular basis for the prevention of alcohol-induced stress by the polyphenols in alcoholic beverages.
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Ruiqing
R
Yao
Department of Biological Engineering, Maebashi Institute of Technology, Maebashi-shi, Gunma, Japan ; Kanagawa Academy of Science and Technology, Takatsu-ku, Kawasaki-shi, Kanagawa, Japan.
Akihito
A
Yasuoka
Kanagawa Academy of Science and Technology, Takatsu-ku, Kawasaki-shi, Kanagawa, Japan.
Asuka
A
Kamei
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Shota
S
Ushiama
Institute for Health Care Science, Suntory Wellness Ltd., Shimamoto-cho, Mishima-gun, Osaka, Japan.
Yoshinori
Y
Kitagawa
Institute for Health Care Science, Suntory Wellness Ltd., Shimamoto-cho, Mishima-gun, Osaka, Japan.
Tomohiro
T
Rogi
Institute for Health Care Science, Suntory Wellness Ltd., Shimamoto-cho, Mishima-gun, Osaka, Japan.
Hiroshi
H
Shibata
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan ; Kanagawa Academy of Science and Technology, Takatsu-ku, Kawasaki-shi, Kanagawa, Japan.
Keiko
K
Abe
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Takumi
T
Misaka
D000434:Alcoholic Beverages; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002492:Central Nervous System Depressants / Q000494:pharmacology; D000090702:Constitutive Androstane Receptor; D004610:Ellagic Acid / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D005235:Fatty Liver, Alcoholic / Q000235:genetics / Q000517:prevention & control*; D005260:Female; D063990:Gene Ontology; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics; D051379:Mice; D008809:Mice, Inbred C3H; D018345:Mice, Knockout; D020411:Oligonucleotide Array Sequence Analysis; D059808:Polyphenols / Q000494:pharmacology*; D018160:Receptors, Cytoplasmic and Nuclear / Q000172:deficiency* / Q000235:genetics; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology; D059467:Transcriptome / Q000187:drug effects / Q000235:genetics
D000975:Antioxidants; D002492:Central Nervous System Depressants; D000090702:Constitutive Androstane Receptor; D059808:Polyphenols; D018160:Receptors, Cytoplasmic and Nuclear; D013267:Stilbenes; D004610:Ellagic Acid; D000431:Ethanol; D000077185:Resveratrol