Evaluation of a commercial real-time PCR for the detection of extended spectrum β-lactamase genes.
We investigated the performance of a real-time PCR for the detection of extended spectrum β-lactamase genes in Enterobacteriaceae (Check-MDR ESBL PCR). Results from micro-arrays were considered as the gold standard. An analysis on 489 isolates resulted in a sensitivity of 98.9 % and a specificity of 100 % for the PCR.
Laboratory for Microbiology and Infection Control, Amphia Hospital, Breda, The Netherlands.
Ina
I
Willemsen
Laboratory for Microbiology and Infection Control, Amphia Hospital, Breda, The Netherlands.
Linda
L
Hille
Laboratory for Microbiology and Infection Control, Amphia Hospital, Breda, The Netherlands.
Angela
A
Vrolijk
Department for Microbiology and Infection Control, Saint Fransiscus Hospital, Roosendaal, The Netherlands.|Laboratory for Microbiology and Infection Control, Amphia Hospital, Breda, The Netherlands.
Anneke
A
Bergmans
Department for Microbiology and Infection Control, VUmc Medical Center, Amsterdam, The Netherlands.|Department for Microbiology and Infection Control, Saint Fransiscus Hospital, Roosendaal, The Netherlands.|Laboratory for Microbiology and Infection Control, Amphia Hospital, Breda, The Netherlands.
Jan
J
Kluytmans
D001431:Bacteriological Techniques / Q000379:methods*; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000382:microbiology; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000235:genetics*
D001618:beta-Lactamases