Chemical constituents of Euphorbia tibetica and their biological activities.
OBJECTIVE
To investigate the chemical constituents and their biological activities of the aerial parts of Euphorbia tibetica.


METHODS
Compounds were isolated and purified by various chromatographic methods, and their structures were elucidated through the use of extensive spectroscopic techniques including 2D-NMR, the structures of compounds 5 and 6 were confirmed by single-crystal X-ray crystallography. Bioactivities of all the isolated compounds were evaluated by the MTT method on A549 and anti-angiogenesis assay.


RESULTS
One new compound, methyl 4-epi-shikimate-3-O-gallate (1), together with twenty-three known constituents (2-24) were isolated from the aerial parts of E. tibetica.


CONCLUSIONS
Compound 1 is new, and the other compounds were isolated from this plant for the first time. Compounds 5-7, 9, 11, 17, 18 and 20 exhibited inhibitory effects on the growth of human lung cancer cell A549 and compounds 5, 7, 12, 13, 17 and 19 showed anti-angiogenic effects in a zebrafish model.
Key Laboratory of Economic Plants and Biotechnology; Germplasm Bank of Wild Species in Southwest China; Institute of Tibetan Plateau Research at Kunming, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
Yang
Y
Da-Song
Biology Institute of Shandong Academy of Sciences, Jinan 250014, China.
He
H
Qiu-Xia
Key Laboratory of Economic Plants and Biotechnology; Germplasm Bank of Wild Species in Southwest China; Institute of Tibetan Plateau Research at Kunming, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
Yang
Y
Yong-Ping
Biology Institute of Shandong Academy of Sciences, Jinan 250014, China. Electronic address: liukechun2000@163.com.
Liu
L
Ke-Chun
Key Laboratory of Economic Plants and Biotechnology; Germplasm Bank of Wild Species in Southwest China; Institute of Tibetan Plateau Research at Kunming, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. Electronic address: li_xiaoli11@mail.kib.ac.cn.
Li
L
Xiao-Li
D020533:Angiogenesis Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology*; D028481:Euphorbia / Q000737:chemistry*; D006131:Growth Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D015394:Molecular Structure; D015027:Zebrafish
D020533:Angiogenesis Inhibitors; D004365:Drugs, Chinese Herbal; D006131:Growth Inhibitors
Cytotoxicity; Diterpenes; Euphorbia tibetica; Euphorbiaceae; R284.1; Shikimate derivatives