Novel membrane-based biotechnological alternative process for succinic acid production and chemical synthesis of bio-based poly (butylene succinate).
Succinic acid was produced in a novel membrane-based fermentation and separation integrated system. With this integrated system, product inhibition was alleviated by removing acids and replenishing fresh broth. High cell density maintain for a longer time from 75 to 130h and succinic acid concentration increased from 53 to 73g/L. In the developed separation process, succinic acid was crystallized at a recovery of 85-90%. The purity of the obtained succinic acid crystals reached 99.4% as found by HPLC and (1)H NMR analysis. A crystallization experiment indicated that among by-products glucose had a negative effect on succinic acid crystallization. Poly (butylene succinate) (PBS) was synthesized using the purified succinic acid and (1)H NMR analysis confirmed that the composition of the synthesized PBS is in agreement with that from petro-based succinic acid.
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China; Graduate University of Chinese Academy of Sciences, Beijing 100049, PR China.
Caixia
C
Wang
Department of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, PR China.
Wei
W
Ming
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China; Graduate University of Chinese Academy of Sciences, Beijing 100049, PR China.
Daojiang
D
Yan
Department of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, PR China.
Congcong
C
Zhang
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
Maohua
M
Yang
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China; Graduate University of Chinese Academy of Sciences, Beijing 100049, PR China.
Yilan
Y
Liu
Department of Chemical Engineering, Institute of Polymer Science and Engineering, Tsinghua University, Beijing 100084, PR China.
Yu
Y
Zhang
Department of Chemical Engineering, Institute of Polymer Science and Engineering, Tsinghua University, Beijing 100084, PR China.
Baohua
B
Guo
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
Yinhua
Y
Wan
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China. Electronic address: jmxing@home.ipe.ac.cn.
Jianmin
J
Xing
D001709:Biotechnology / Q000379:methods*; D002072:Butylene Glycols / Q000138:chemical synthesis*; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism; D004951:Esterification; D005285:Fermentation; D008567:Membranes, Artificial; D011108:Polymers / Q000138:chemical synthesis*; D012996:Solutions; D019802:Succinic Acid / Q000138:chemical synthesis*; D013997:Time Factors
D002072:Butylene Glycols; D008567:Membranes, Artificial; D011108:Polymers; D012996:Solutions; C089797:bionole; D019802:Succinic Acid
Integrated process; Membrane separation; Poly (butylene succinate) synthesis; Succinic acid