Effects of nicotine on proliferation and survival in human umbilical cord mesenchymal stem cells.
Cigarette smoking is known to have negative effects on tissue repair and healing. The aim of this study is to investigate the effects of nicotine in human umbilical cord mesenchymal stem cells (MSCs). After nicotine treatment, MSCs became pyknotic, vacuoles appeared in the cytoplasm and nucleus, and the nuclear boundary became fuzzy as observed using atomic force microscopy. Cell proliferation was inhibited in a dose-dependent manner (P < 0.05 for all concentrations). The proportion of apoptotic MSCs was significantly increased in a dose-dependent manner. The mitochondrial membrane potential was significantly decreased (P < 0.05). Nicotine-treated MSCs had a significantly higher G0/G1 ratio (P < 0.05). Peptide mass fingerprinting identified 27 proteins that were differentially expressed between MSCs with and without nicotine treatment. These nicotine exerted toxic effects on MSCs are likely related, at least in part, to the altered expression of multiple proteins that are essential to the health and proliferation of these cells.
Department of Hematology, The First Affiliated Hospital of Jinan University, Guangzhou, 510630, People's Republic of China. thlz@jnu.edu.cn.
Hui-Lan
HL
Zeng
Yong-Liang
YL
Qin
Hui-Zhong
HZ
Chen
Qian-Qian
QQ
Bu
Yang
Y
Li
Qi
Q
Zhong
Xin-Ai
XA
Han
Jie
J
Chen
Pan-Xi
PX
Yu
Ge-Xiu
GX
Liu
D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D015536:Down-Regulation / Q000187:drug effects; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000502:physiology*; D009538:Nicotine / Q000633:toxicity*; D020543:Proteome / Q000378:metabolism; D014470:Umbilical Cord / Q000166:cytology
D020543:Proteome; D009538:Nicotine
Apoptosis; Mesenchymal Stem Cells; Nicotine; Proliferation; Proteome; Ultrastructure