Vaccination of monoglycosylated hemagglutinin induces cross-strain protection against influenza virus infections.
The 2009 H1N1 pandemic and recent human cases of H5N1, H7N9, and H6N1 in Asia highlight the need for a universal influenza vaccine that can provide cross-strain or even cross-subtype protection. Here, we show that recombinant monoglycosylated hemagglutinin (HAmg) with an intact protein structure from either seasonal or pandemic H1N1 can be used as a vaccine for cross-strain protection against various H1N1 viruses in circulation from 1933 to 2009 in mice and ferrets. In the HAmg vaccine, highly conserved sequences that were originally covered by glycans in the fully glycosylated HA (HAfg) are exposed and thus, are better engulfed by dendritic cells (DCs), stimulated better DC maturation, and induced more CD8+ memory T cells and IgG-secreting plasma cells. Single B-cell RT-PCR followed by sequence analysis revealed that the HAmg vaccine activated more diverse B-cell repertoires than the HAfg vaccine and produced antibodies with cross-strain binding ability. In summary, the HAmg vaccine elicits cross-strain immune responses that may mitigate the current need for yearly reformulation of strain-specific inactivated vaccines. This strategy may also map a new direction for universal vaccine design.
Genomics Research Center, Academia Sinica, Taipei 115, Taiwan.
Juine-Ruey
JR
Chen
Yueh-Hsiang
YH
Yu
Yung-Chieh
YC
Tseng
Wan-Ling
WL
Chiang
Ming-Feng
MF
Chiang
Yi-An
YA
Ko
Yi-Kai
YK
Chiu
Hsiu-Hua
HH
Ma
Chung-Yi
CY
Wu
Jia-Tsrong
JT
Jan
Kuo-I
KI
Lin
Che
C
Ma
Chi-Huey
CH
Wong
D000818:Animals; D001483:Base Sequence; D002853:Chromatography, Liquid; D003001:Cloning, Molecular; D015195:Drug Design; D004797:Enzyme-Linked Immunosorbent Assay; D058501:Enzyme-Linked Immunospot Assay; D005260:Female; D005289:Ferrets; D005434:Flow Cytometry; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000494:pharmacology*; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology*; D007252:Influenza Vaccines / Q000494:pharmacology*; D007251:Influenza, Human / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D009975:Orthomyxoviridae / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013045:Species Specificity; D053719:Tandem Mass Spectrometry
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines
broadly neutralizing antibody; glycoprotein engineering