Erythropoietin ameliorates genetamicin-induced renal toxicity: A biochemical and histopathological study.
BACKGROUND
Investigations have attempted to modify the outcome of tubular injury by either ameliorating renal tubular damage or promoting tubular regeneration in the case of acute tubular necrosis.


OBJECTIVE
We investigated the protective effect of Eprex an erythropoietin analogue on tubular injury induced by gentamicin (GM).


METHODS
Forty male Wistar rats were randomly divided into four groups. In group 1,rats were served as a sham group. In group 2, rats were injected intraperitoneally with 100 mg/kg of GM for 10 consecutive days (positive control group) and then were sacrificed. In group 3, rats received GM for 10 days then Eprex 100U/kg was injected intraperitoneally for the next 10 days and then they were sacrificed at the day 20th. In group 4 rats were injected a combination of GM (80 mg/kg) and Eprex 100U/kg intraperitoneally for 10 days and then were sacrificed.


RESULTS
The results indicated that, Eprex prevented the increase in serum creatinine (Cr) and blood urea nitrogen (BUN). The effect of Eprex on damage score, showed that co-administration of GM and Eprex (group 3 and 4) reduced the kidney tissue damage compared to positive control group (P<0.05). This result indicat that Eprex potentially can reduce or prevent the kidney tissue damage.


CONCLUSIONS
Ameliorative effect of Eprex when the drug was given in combination with GM and also when the drug was applied after GM-induced tubular damage, revealed the renoprotective potency of Eprex. Eprex is a promising drug to prevent or attenuate tubular damage induced by GM or other nephrotoxic agents which act through the same mechanisms as gentamicin.
Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Mahmoud
M
Rafieian-Kopaei
Department of Nephrology, Division of Nephropathology, Isfahan University of Medical Sciences, Isfahan, Iran.
Hamid
H
Nasri
Water and Electrolytes Research Center, Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran.
Mehdi
M
Nematbakhsh
Department of Clinical Pathology, Isfahan University of Medical Sciences, Isfahan , Iran.
Azar
A
Baradaran
Isfahan Kidney Disease Research Center, Isfahan University of Medical Sciences, Isfahan , Iran.
Alaleh
A
Gheissari
Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Hamid
H
Rouhi
Department of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Seyed Mohammad
SM
Ahmadi Soleimani
Medical Physics and Medical Engineering Department, Isfahan University of Medical Sciences, Isfahan, Iran.
Milad
M
Baradaran-Ghahfarokhi
Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Fatemeh
F
Ghaed-Amini
Department of Nephrology, Tabriz University of Medical Sciences, Tabriz, Iran.
Mohammadreza
M
Ardalan
Erythropoietin; Gentamicin; Tubular toxicity