[A comparative study of the cytotoxicity of five dental bonding agents to human periodontal ligament cells].
OBJECTIVE
To compare the cytotoxicity of 5 different dental bonding agents to human periodontal ligament cells(HPDLCs).


METHODS
Primary cultured HPDLCs were exposed to different concentrations (100%,50% and 25%) of Super Bond, Clearfil SE Bond, G-Bond, Single Bond2, and Adper Easy One for different times (24 h, 48 h and 72 h). Cytotoxicity of 5 different agents to HPDLCs were observed by MTT method. The data was analyzed using SPSS 19.0 software package.


RESULTS
Cytotoxicity of 5 dentin bonding agents to HPDLCs were Super Bond<Clearfil SE Bond<G-Bond<Adper Easy One<Single Bond2. Cytotoxicity was affected by the exposure time and concentration. Cytotoxicity effect was 25%<50%<100% and 24 h<48 h<72 h. Significant difference was found between 24h and 48h(P<0.05) but no significant difference was found between 48h and 72h (P>0.05).


CONCLUSIONS
Cytotoxicity of 5 dentin bonding agents are different. Cytotoxicity of Super-bond C&B,G-Bond and Clearfil SE Bond are weak, while cytotoxicity of Single Bond2 is the strongest among the 5 agents. Supported by Medical Leading Talents and Innovation Team Project of Jiangsu Province (LJ201110) and Natural Science Foundation of Jiangsu Province (SBK201340904).
Department of Endodontics,Nanjing Stomatological Hospital, Central Laboratory of Stomatological School of Nanjing University. Nanjing 210008, Jiangsu Province, China.E-mail:lijunliang10@163.com.
Jun-liang
JL
Li
Wen-mei
WM
Wang
Jiu-yu
JY
Ge
Jun
J
Ji
Tian-cong
TC
Wang
D001896:Boron Compounds; D001840:Dental Bonding; D003804:Dentin; D017220:Dentin-Bonding Agents; D006801:Humans; D008689:Methacrylates; D008768:Methylmethacrylates; D010513:Periodontal Ligament; D019279:Resin Cements
D001896:Boron Compounds; C438313:Clearfil SE Bond; D017220:Dentin-Bonding Agents; C498353:G-Bond; D008689:Methacrylates; D008768:Methylmethacrylates; D019279:Resin Cements; C049495:Super-bond