Identification and biosynthesis of new acyloins from the thermophilic bacterium Thermosporothrix hazakensis SK20-1(T).
Two new acyloin compounds were isolated from the thermophilic bacterium Thermosporothrix hazakensis SK20-1(T) . Genome sequencing of the bacterium and biochemical studies identified the thiamine diphosphate (TPP)-dependent enzyme Thzk0150, which is involved in the formation of acyloin. Through extensive analysis of the Thzk0150-catalyzed reaction products, we propose a putative reaction mechanism involving two substrates: 4-methyl-2-oxovalerate as an acyl donor and phenyl pyruvate as an acyl acceptor.
Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 (Japan).
Jin-Soo
JS
Park
Noritaka
N
Kagaya
Junko
J
Hashimoto
Miho
M
Izumikawa
Shuhei
S
Yabe
Kazuo
K
Shin-Ya
Makoto
M
Nishiyama
Tomohisa
T
Kuzuyama
D001426:Bacterial Proteins / Q000378:metabolism; D055162:Biocatalysis; D002176:Candida albicans / Q000187:drug effects; D041862:Chloroflexi / Q000737:chemistry* / Q000378:metabolism; D005233:Fatty Alcohols / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D006588:Hexanones / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D007651:Keto Acids / Q000737:chemistry / Q000378:metabolism; D010667:Phenylpyruvic Acids / Q000737:chemistry / Q000378:metabolism
D001426:Bacterial Proteins; D005233:Fatty Alcohols; D006588:Hexanones; D007651:Keto Acids; D010667:Phenylpyruvic Acids; C096624:sattabacin; C011159:acyloin; C013082:alpha-ketoisocaproic acid; C031606:phenylpyruvic acid
Thermosporothrix hazakensis; acyloins; biosynthesis; enzymes; thermophilic bacteria