Uptake and biological effects of synthetic glucocorticoids in common carp (Cyprinus carpio).
Uptake and biological effects of synthetic glucocorticoids (GCs) were analyzed using common carp (Cyprinus carpio). Fish were exposed to clobetasol propionate (CP) or clobetasone butyrate (CB) individually or in mixture at 1 μg L(-1) for 21 days. Bioconcentration factor (BCF) of CB was calculated as 100, and BCF of CP was less than 16. No effects were found in fish erythrocyte and leukocyte numbers and serum glucose levels after exposure to the selected GCs. On the other hand, serum concentrations of free amino acids significantly increased in GC-exposed groups. Thus, exposures to synthetic GCs at relatively low concentrations seemed to cause enhancement of protein degradation and subsequent increase of serum free amino acids without a corresponding increase in serum glucose levels, an effect which might be related to partial induction of gluconeogenesis by GC.
Center for Marine Environmental Studies (CMES), Ehime University, 2-5 Bunkyo-cho, Matsuyama 790-8577, Japan. Electronic address: kei_n@ehime-u.ac.jp.
Kei
K
Nakayama
Chemicals Evaluation and Research Institute, Japan, CERI Kurume, 3-2-7 Miyanojin, Kurume 839-0801, Japan.
Yoshiyuki
Y
Inoue
Chemicals Evaluation and Research Institute, Japan, CERI Kurume, 3-2-7 Miyanojin, Kurume 839-0801, Japan.
Naomi
N
Ikeda
Chemicals Evaluation and Research Institute, Japan, CERI Kurume, 3-2-7 Miyanojin, Kurume 839-0801, Japan.
Naoki
N
Hashizume
Chemicals Evaluation and Research Institute, Japan, CERI Kurume, 3-2-7 Miyanojin, Kurume 839-0801, Japan.
Hidekazu
H
Murakami
Chemicals Evaluation and Research Institute, Japan, CERI Kurume, 3-2-7 Miyanojin, Kurume 839-0801, Japan.
Takeshi
T
Ishibashi
Center for Marine Environmental Studies (CMES), Ehime University, 2-5 Bunkyo-cho, Matsuyama 790-8577, Japan.
Hirofumi
H
Ikeda
Center for Marine Environmental Studies (CMES), Ehime University, 2-5 Bunkyo-cho, Matsuyama 790-8577, Japan.
Tomohiko
T
Isobe
Center for Marine Environmental Studies (CMES), Ehime University, 2-5 Bunkyo-cho, Matsuyama 790-8577, Japan.
Shin-Ichi
S
Kitamura
National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba 305-8506, Japan.
Go
G
Suzuki
D000596:Amino Acids / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D002347:Carps / Q000378:metabolism* / Q000502:physiology; D002990:Clobetasol / Q000031:analogs & derivatives* / Q000493:pharmacokinetics* / Q000494:pharmacology; D004906:Erythrocyte Count; D005938:Glucocorticoids / Q000493:pharmacokinetics* / Q000494:pharmacology; D007958:Leukocyte Count; D059748:Proteolysis / Q000187:drug effects
D000596:Amino Acids; D001786:Blood Glucose; D005938:Glucocorticoids; C010893:clobetasone butyrate; D002990:Clobetasol
Bioconcentration factor; Blood cell composition; Common carp; Free amino acid; Glucocorticoid; Glucose