Electroanalytical quantification of total dsDNA extracted from human sample using, an ionic liquid modified, carbon nanotubes paste electrode.
Direct electrochemistry of dsDNA has been achieved by using an ionic liquid 1-butyl-4-methylpyridinium hexafluorophosphate modified carbon nanotubes paste electrode (IL-CNTPE). Oxidation peaks appeared at 0.93 and 1.26 V (vs. Ag/AgCl) on the IL- CNTPE after preconcentration of dsDNA in pH 5.0 acetate buffer, which were attributed to the oxidation of guanine and adenine residues on the dsDNA molecule structure. Based on the signal of guanine, under the optimal conditions, very low levels of dsDNA can be detected after 60s accumulation with detection limits of 0.249 mg L(-) 16 pM. Additionally, human DNA from a healthy volunteer is determined by use of the IL-CNTPE and it is found to be 40±2, 14 pM.
Analytical Chemistry Laboratory, Aristotle University of Thessaloniki, Thessaloniki, 54124, Greece.
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C
Serpi
Laboratory of Forensic Medicine and Toxicology, School of Medicine, Greece.
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Kovatsi
Analytical Chemistry Laboratory, Aristotle University of Thessaloniki, Thessaloniki, 54124, Greece. Electronic address: girousi@chem.auth.gr.
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Girousi
D000327:Adsorption; D004247:DNA / Q000302:isolation & purification*; D004566:Electrodes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D052578:Ionic Liquids; D037742:Nanotubes, Carbon
D052578:Ionic Liquids; D037742:Nanotubes, Carbon; D004247:DNA
1-Butyl-4-methylpyridinium hexafluorophosphate; Carbon nanotubes paste electrode; Direct electrochemistry; Double stranded DNA; Ionic liquid