Poly (glycerol sebacate) elastomer supports osteogenic phenotype for bone engineering applications.
For bone engineering, the optimal scaffolding material and composition has yet to be elucidated. In this study, we investigated poly (glycerol sebacate) (PGS), an elastomer known primarily for its soft tissue regeneration ability, as a suitable substrate to support osteo-precursor cell attachment and function. We synthesized PGS in the form of sheets where MC3T3-E1 cells were seeded in three different densities of 25,000, 50,000 and 100,000 cells mm(-3) and we investigated the cells/scaffold constructs for their cellular proliferation, matrix deposition, maturation, mineralization and their mechanical compression strength at 24 h and two and four weeks. MC3T3-E1 cells proliferated, synthesized a collagenous matrix and expressed osteogenic markers Runx2, bone sialoprotein and osteocalcin according to their initial seeding density on PGS. We conclude that PGS can support the osteoblastic phenotype in vitro and is a promising osteoconductive substrate for bone regeneration research and for future clinical translation.
Department of Oral Biology, School of Dental Medicine, Center for Craniofacial Regeneration, University of Pittsburgh, Pittsburgh, PA, USA.
Samer H
SH
Zaky
Catherine K
CK
Hangadora
Mauro A
MA
Tudares
Jin
J
Gao
Adrianna
A
Jensen
Yadong
Y
Wang
Charles
C
Sfeir
Alejandro J
AJ
Almarza
D016475:3T3 Cells; D000818:Animals; D001842:Bone and Bones / Q000378:metabolism; D049109:Cell Proliferation; D003094:Collagen / Q000737:chemistry; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D003651:Decanoates / Q000737:chemistry*; D020724:Elastomers / Q000737:chemistry*; D005990:Glycerol / Q000031:analogs & derivatives* / Q000737:chemistry; D058574:Integrin-Binding Sialoprotein / Q000378:metabolism; D051379:Mice; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis; D010641:Phenotype; D011108:Polymers / Q000737:chemistry*; D012038:Regeneration; D013314:Stress, Mechanical; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
D050659:Core Binding Factor Alpha 1 Subunit; D003651:Decanoates; D020724:Elastomers; D058574:Integrin-Binding Sialoprotein; D011108:Polymers; C493716:Runx2 protein, mouse; C469892:poly(glycerol-sebacate); D015675:Osteocalcin; D003094:Collagen; D005990:Glycerol