Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.
Nickel is an essential micronutrient due to its involvement in many enzymatic reactions as a cofactor. However, excess of this element is toxic to biological systems. Here, we constructed a cDNA library from Beta maritima and screened it in the yeast system to identify genes that confer resistance to toxic levels of nickel. A cDNA clone (NIC6), which encodes for a putative membrane protein with unknown function, was found to help yeast cells to tolerate toxic levels of nickel. A GFP fused form of Nic6 protein was localized to multivesicular structures in tobacco epidermal cells. Thus, our results suggest a possible role of Nic6 in nickel and intracellular ion homeostasis.
Izmir Institute of Technology, Department of Molecular Biology and Genetics, 35430 Urla, Izmir, Turkey.
Gonensin O
GO
Bozdag
Izmir Institute of Technology, Department of Molecular Biology and Genetics, 35430 Urla, Izmir, Turkey.
Alaattin
A
Kaya
Izmir Institute of Technology, Department of Molecular Biology and Genetics, 35430 Urla, Izmir, Turkey.
Ahmet
A
Koc
Westphalian Wilhelms-University Münster, Institute for Biochemistry and Biotechnology of Plants, D-48143 Münster, Germany.
Gundula A
GA
Noll
Westphalian Wilhelms-University Münster, Institute for Biochemistry and Biotechnology of Plants, D-48143 Münster, Germany.
Dirk
D
Prüfer
Izmir Institute of Technology, Department of Molecular Biology and Genetics, 35430 Urla, Izmir, Turkey. Electronic address: caglarkarakaya@iyte.edu.tr.
Hüseyin Caglar
HC
Karakaya
D000595:Amino Acid Sequence; D001483:Base Sequence; D027461:Beta vulgaris / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics*; D015723:Gene Library; D017343:Genes, Plant; D008658:Inactivation, Metabolic / Q000235:genetics; D008969:Molecular Sequence Data; D009532:Nickel / Q000378:metabolism* / Q000633:toxicity; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism
D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D009532:Nickel
Beta maritima; Heavy metal toxicity; Ion homeostasis; Nickel; Transporter protein; Yeast