Conducting miller-urey experiments.
In 1953, Stanley Miller reported the production of biomolecules from simple gaseous starting materials, using an apparatus constructed to simulate the primordial Earth's atmosphere-ocean system. Miller introduced 200 ml of water, 100 mmHg of H2, 200 mmHg of CH4, and 200 mmHg of NH3 into the apparatus, then subjected this mixture, under reflux, to an electric discharge for a week, while the water was simultaneously heated. The purpose of this manuscript is to provide the reader with a general experimental protocol that can be used to conduct a Miller-Urey type spark discharge experiment, using a simplified 3 L reaction flask. Since the experiment involves exposing inflammable gases to a high voltage electric discharge, it is worth highlighting important steps that reduce the risk of explosion. The general procedures described in this work can be extrapolated to design and conduct a wide variety of electric discharge experiments simulating primitive planetary environments.
School of Chemistry and Biochemistry, Georgia Institute of Technology.
Eric T
ET
Parker
James H
JH
Cleaves
Aaron S
AS
Burton
Daniel P
DP
Glavin
Jason P
JP
Dworkin
Manshui
M
Zhou
Jeffrey L
JL
Bada
Facundo M
FM
Fernández
D000596:Amino Acids / Q000138:chemical synthesis*; D000641:Ammonia / Q000737:chemistry*; D055664:Electrochemical Techniques; D019418:Evolution, Chemical; D008401:Gas Chromatography-Mass Spectrometry; D008697:Methane / Q000737:chemistry*; D009584:Nitrogen / Q000737:chemistry; D000069497:Origin of Life
D000596:Amino Acids; D000641:Ammonia; D009584:Nitrogen; D008697:Methane