Plasminogen activator inhibitor type 1 expression induced by lipopolysaccharide of Porphyromonas gingivalis in human gingival fibroblast.
In the gingival tissues of patients with periodontitis, inflammatory responses are mediated by a wide variety of genes. In our previous screening study, plasminogen activator inhibitor type 1 (PAI-1) mRNA binding protein expression was increased in gingiva from periodontitis patients. In this study, we further investigated the signaling pathway involved in PAI-1 expression induced by Porphyromonas gingivalis LPS (Pg LPS) in human gingival fibroblasts (HGF). When HGFs were treated with Pg LPS, both PAI-1 mRNA expression and PAI-1 protein were induced in a dose-dependent manner. Pg LPS induced NF-κB activation and the expressions of PAI-1 mRNA and protein were suppressed by pretreating with a NF-κB inhibitor. Pg LPS also induced ERK, p38, and JNK activation, and Pg LPS-induced PAI-1 expression was inhibited by ERK/p38/JNK inhibitor pretreatment. In conclusion, Pg LPS induced PAI-1 expression through NF-κB and MAP kinases activation in HGF.
Department of Oral Microbiology, Pusan National University, Yangsan, 626-810, Republic of Korea.
Hee Sam
HS
Na
Eun J
EJ
Lim
So Y
SY
Jeong
Mi H
MH
Ryu
Mi Hee
MH
Park
Jin
J
Chung
D001826:Body Fluids / Q000166:cytology / Q000378:metabolism; D002460:Cell Line; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005881:Gingiva / Q000737:chemistry / Q000166:cytology*; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D017395:Plasminogen Activator Inhibitor 1 / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D016966:Porphyromonas gingivalis / Q000737:chemistry*
D008070:Lipopolysaccharides; D017395:Plasminogen Activator Inhibitor 1; D048049:Extracellular Signal-Regulated MAP Kinases