Methods for measuring antibody-dependent cell-mediated cytotoxicity in vitro.
Antibody-dependent cell-mediated cytotoxicity (ADCC) is a relevant characteristic to measure for a number of therapeutic monoclonal antibodies (mAbs) under development. ADCC is a mechanism by which antibody-opsonized, infected, or cancerous cells are destroyed by FcγRIII (CD16)-expressing effector cells. Here we describe three methods that can be used to quantify the ADCC activity of mAbs by measuring distinct aspects of the ADCC mechanism.
Analytical Development and Quality Control, Genentech Inc., South San Francisco, CA, USA.
Aaron S
AS
Miller
Max L
ML
Tejada
Hélène
H
Gazzano-Santoro
D000920:Antibody-Dependent Cell Cytotoxicity / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology
D016207:Cytokines