End-binding protein 1 (EB1) up-regulation is an early event in colorectal carcinogenesis.
End-binding protein (EB1) is a microtubule protein that binds to the tumor suppressor adenomatous polyposis coli (APC). While EB1 is implicated as a potential oncogene, its role in cancer progression is unknown. Therefore, we analyzed EB1/APC expression at the earliest stages of colorectal carcinogenesis and in the uninvolved mucosa ("field effect") of human and animal tissue. We also performed siRNA-knockdown in colon cancer cell lines. EB1 is up-regulated in early and field carcinogenesis in the colon, and the cellular/nano-architectural effect of EB1 knockdown depended on the genetic context. Thus, dysregulation of EB1 is an important early event in colon carcinogenesis.
Biomedical Engineering Department, Northwestern University, Evanston, IL 60208, USA. Electronic address: stypula@u.northwestern.edu.
Yolanda
Y
Stypula-Cyrus
Biomedical Engineering Department, Northwestern University, Evanston, IL 60208, USA.
Nikhil N
NN
Mutyal
Department of Medicine, Boston Medical Center, Boston, MA 02118, USA.
Mart
M
Dela Cruz
Department of Internal Medicine, NorthShore University Health System, Evanston, IL 60201, USA.
Dhananjay P
DP
Kunte
Biomedical Engineering Department, Northwestern University, Evanston, IL 60208, USA.
Andrew J
AJ
Radosevich
Department of Medicine, Boston Medical Center, Boston, MA 02118, USA.
Ramesh
R
Wali
Department of Medicine, Boston Medical Center, Boston, MA 02118, USA. Electronic address: hkroy@bu.edu.
Hemant K
HK
Roy
Biomedical Engineering Department, Northwestern University, Evanston, IL 60208, USA.
Vadim
V
Backman
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000236:Adenoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D000818:Animals; D017209:Apoptosis; D063646:Carcinogenesis / Q000378:metabolism*; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D003599:Cytoskeleton / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D008297:Male; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D046888:Tissue Array Analysis; D015854:Up-Regulation
C500985:MAPRE1 protein, human; D008869:Microtubule-Associated Proteins
Adenomatous polyposis coli; Colon cancer; End-binding protein 1; Field carcinogenesis; Sub-diffractional structure