Effect of acupuncture on CXCL8 receptors in rats suffering from embryo implantation failure.
To observe the effect of acupuncture on CXCL8 receptors (CXCR1 and CXCR2) in rat endometrium experiencing embryo implantation failure, 72 pregnant rats were randomly divided into four groups: normal group (N), embryo implantation failure group (M), acupuncture treatment group (A), and progestin treatment group (W). Then the rats in each group were equally randomized into a day-6 (D6) group, a day-8 (D8) group, and a day-10 (D10) group. The rats in group M, group A, and group W were treated with mifepristone-sesame oil solution on day 1, while the rats in group N were injected with the same amount of sesame oil. Meanwhile, "Housanli" and "Sanyinjiao" were selected for acupuncture. From day 1 to the time of death, the rats in group A were fastened up and then acupuncture was administered while the rats in group N and group M were only fixed, and the rats in group W were given progestin. The number of implanted embryos was calculated. The expression of CXCR1 and CXCR2 in rat endometrium was detected by immunohistochemistry, Western blotting and real-time PCR. Compared to group N, the average number of implanted embryos, the protein and mRNA expression of CXCR1 (D6, D8 and D10), and the protein and mRNA expression of CXCR2 (D8 and D10) in rat endometrium were significantly decreased in group M. Compared to group M, there was significant elevation in the average number of implanted embryos, the protein expression (D6, D8 and D10) and mRNA expression (D8) of CXCR1 in rat endometrium of group A, and the protein expression (D8 and D10) and mRNA expression (D8) of CXCR2 in rat endometrium of group W. These findings indicated that acupuncture can increase the number of implanted embryos in rats of embryo implantation failure, which may be relevant with up-regulation the expression of CXCR1 and CXCR2 at maternal-fetal interface of rats with embryo implantation failure.
Institute of Integrated Traditional Chinese Medicine and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
Wei-Na
WN
Gao
Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
Wei
W
Yang
Institute of Integrated Traditional Chinese Medicine and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
Ya-Fei
YF
Liu
Institute of Integrated Traditional Chinese Medicine and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
Xiao
X
Tang
Institute of Integrated Traditional Chinese Medicine and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
Yue
Y
Guo
Institute of Integrated Traditional Chinese Medicine and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
Li-Jun
LJ
Wang
Institute of Integrated Traditional Chinese Medicine and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
Ming-Min
MM
Zhang
Institute of Integrated Traditional Chinese Medicine and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. gyhuang@tjh.tjmu.edu.cn.
Guang-Ying
GY
Huang
D015670:Acupuncture Therapy / Q000379:methods*; D000818:Animals; D015153:Blotting, Western; D010064:Embryo Implantation / Q000187:drug effects / Q000235:genetics*; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006727:Hormone Antagonists / Q000494:pharmacology; D007150:Immunohistochemistry; D015735:Mifepristone / Q000494:pharmacology; D011247:Pregnancy; D011372:Progestins / Q000494:pharmacology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D023062:Receptors, Interleukin-8A / Q000235:genetics* / Q000378:metabolism; D023063:Receptors, Interleukin-8B / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D016896:Treatment Outcome; D015854:Up-Regulation / Q000187:drug effects
D006727:Hormone Antagonists; D011372:Progestins; D023062:Receptors, Interleukin-8A; D023063:Receptors, Interleukin-8B; D015735:Mifepristone