Tempol attenuates atherosclerosis associated with metabolic syndrome via decreased vascular inflammation and NADPH-2 oxidase expression.
Oxidative stress is an important factor in the generation of vascular injury in atherosclerosis. Chronic administration of fructose in rodents is able to facilitate oxidative damage. In the present study we evaluated the role of Tempol, a superoxide dismutase mimetic, on the effect of high fructose intake in apolipoprotein E-deficient (ApoE-KO) mice. Rodents were fed with fructose overload (FF, 10% w/v) for 8 weeks and treated with Tempol 1 mg/kg/day the latest 4 weeks. Tempol revert the pro-oxidant effects caused by FF, diminished lipid peroxidation and impaired vascular NADPH oxidase system through the downregulation of p47phox expression in the vascular wall. Tempol inhibited the expression of vascular adhesion molecule 1 (VCAM-1) in aorta and reduced the development of atheroma plaques. Our results indicate that tempol attenuates oxidative stress by interfering with the correct assembly of Nox2 oxidase complex in the vascular wall and is able to reduce atherosclerosis. Thus tempol represents a potential therapeutic target for preventing risk factors associated with metabolic syndrome.
Vascular Biology Lab, Institute of Medicine and Experimental Biology of Cuyo (IMBECU) CONICET, School of Medical Sciences, National University of Cuyo , Mendoza , Argentina.
B
B
Cannizzo
I
I
Quesada
R
R
Militello
C
C
Amaya
R
R
Miatello
M
M
Cruzado
C
C
Castro
D000818:Animals; D050197:Atherosclerosis / Q000209:etiology* / Q000378:metabolism; D003497:Cyclic N-Oxides / Q000009:adverse effects*; D007249:Inflammation / Q000378:metabolism; D008297:Male; D024821:Metabolic Syndrome / Q000150:complications*; D051379:Mice; D008810:Mice, Inbred C57BL; D019255:NADPH Oxidases / Q000378:metabolism; D018384:Oxidative Stress; D017382:Reactive Oxygen Species; D013113:Spin Labels
D003497:Cyclic N-Oxides; D017382:Reactive Oxygen Species; D013113:Spin Labels; D019255:NADPH Oxidases; C001803:tempol