Characterization of the cellular localization of C4orf34 as a novel endoplasmic reticulum resident protein.
Human genome projects have enabled whole genome mapping and improved our understanding of the genes in humans. However, many unknown genes remain to be functionally characterized. In this study, we characterized human chromosome 4 open reading frame 34 gene (hC4orf34). hC4orf34 was highly conserved from invertebrate to mammalian cells and ubiquitously expressed in the organs of mice, including the heart and brain. Interestingly, hC4orf34 is a novel ER-resident, type I transmembrane protein. Mutant analysis showed that the transmembrane domain (TMD) of hC4orf34 was involved in ER retention. Overall, our results indicate that hC4orf34 is an ER-resident type I transmembrane protein, and might play a role in ER functions including Ca2+ homeostasis and ER stress.
Department of Biotechnology, College of Life Science and Nano Technology, Hannam University, Daejeon 305-811, Korea.
Mi-Hee
MH
Jun
Department of Ecological Science, College of Ecology and Environment, Kyungpook National University, Sangju 742-711, Korea.
Young-Wu
YW
Jun
Department of Ecological Science, College of Ecology and Environment, Kyungpook National University, Sangju 742-711, Korea.
Kun-Hyung
KH
Kim
Department of Biotechnology, College of Life Science and Nano Technology, Hannam University, Daejeon 305-811, Korea.
Jin-A
JA
Lee
Department of Ecological Science, College of Ecology and Environment, Kyungpook National University, Sangju 742-711, Korea.
Deok-Jin
DJ
Jang
D000595:Amino Acid Sequence; D000818:Animals; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism*; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D021381:Protein Transport / Q000187:drug effects; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014415:Tunicamycin / Q000494:pharmacology
D008565:Membrane Proteins; D011506:Proteins; C000598976:SMIM14 protein, human; D014415:Tunicamycin