Evaluation of a short, on-plate formic acid extraction method for matrix-assisted laser desorption ionization-time of flight mass spectrometry-based identification of clinically relevant yeast isolates.
This report describes a short, on-plate formic acid (FA) extraction method for the identification of clinical yeast isolates using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS). A total of 41.1% (78/190) and 63.7% (121/190) of yeasts were identified using species log score thresholds of >2.0 and >1.9, respectively. Overall, 97.4% (185/190) of yeasts were identified in combination with conventional FA extraction.
UK Clinical Mycology Network (UKCMN) Regional Laboratory, Department of Medical Microbiology, Royal Free Hospital, Pond Street, Hampstead, London, United Kingdom.
Rebecca L
RL
Gorton
Shila
S
Seaton
Purnima
P
Ramnarain
Timothy D
TD
McHugh
Christopher C
CC
Kibbler
D005561:Formates; D006801:Humans; D009181:Mycoses / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D015003:Yeasts / Q000737:chemistry* / Q000145:classification* / Q000302:isolation & purification
D005561:Formates; C030544:formic acid