The testis of the mice C57/BL6 offspring in adulthood have alterations due to maternal caffeine consumption.
OBJECTIVE
To investigate the effects of the maternal caffeine consumption during pregnancy to adult male testis mice offspring.


METHODS
Twenty pregnant mice were divided into control group (c) and caffeine group (cf). dams received daily saline or 20 mg/kg of caffeine subcutaneously. Male offspring were monitored daily until 13th week. The testis were used to evaluate both the proliferation (pcna) and apoptosis (bax); leptin receptor (ob-r); aromatase; follicle stimulating hormone (fshr), luteinizing hormone (lhr) and androgen receptors (ar); steroidogenic acute regulatory protein (star); vascular endothelial growth factor (vegf) and estrogen receptors (erα and erβ) by western blotting. Serum concentrations of testosterone, estradiol and leptin were measured.


RESULTS
There was a significant reduction in food intake and the body mass gain (p<0.05) in the cf ; pcna (p=0.01), fshr (p=0.02), star (p=0.0007), vegf (p=0.009), ar (p=0.03) in the cf. while an increase were note in bax (p=0.01), ob-r (p=0.02), lhr (p=0.04) and in the aromatase (p=0.03) in the cf. only erα and erβ were not changed by maternal caffeine. The serum testosterone levels in the cf offspring were 90% lower than in the c offspring (p=0.04).


CONCLUSIONS
Maternal caffeine consumption has a role and alters the testis of the offspring in adulthood.
State University of Rio de Janeiro, Brazil.
Fernanda Silveira
FS
Cavalcante
UERJ, Rio de JaneiroRJ, Brazil.
Verônica
V
Aiceles
State Department of Health, Rio de Janeiro, Brazil.
Diana de Freitas Serapião
Dde F
Moraes
UERJ, Rio de JaneiroRJ, Brazil.
Jorge Luiz
JL
Alves-Pereira
UERJ, Anatomy Department, Rio de JaneiroRJ, Brazil.
Tatiane Silva
TS
Faria
UERJ, Anatomy Department, Rio de JaneiroRJ, Brazil.
Cristiane da Fonte
Cda F
Ramos
D000818:Animals; D015992:Body Mass Index; D002110:Caffeine / Q000378:metabolism / Q000494:pharmacology*; D004435:Eating / Q000187:drug effects; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D020738:Leptin / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010750:Phosphoproteins / Q000378:metabolism*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011897:Random Allocation; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism; D000098682:Steroidogenic Acute Regulatory Protein
D020738:Leptin; D010750:Phosphoproteins; D018809:Proliferating Cell Nuclear Antigen; D000098682:Steroidogenic Acute Regulatory Protein; D002110:Caffeine; D013739:Testosterone; D004958:Estradiol; D005640:Follicle Stimulating Hormone