Expression and functional analysis of putative vacuolar Ca2+-transporters (CAXs and ACAs) in roots of salt tolerant and sensitive rice cultivars.
Vacuolar Ca2+-transporters could play an important role for salt tolerance in rice (Oryza sativa L.) root. Here, we compared the expression profiles of putative vacuolar cation/H+ exchanger (CAX) and calmodulin-regulated autoinhibited Ca2+-ATPase (ACA) in rice roots of salt tolerant cv. Pokkali and salt sensitive cv. IR29. In addition to five putative vacuolar CAX genes in the rice genome, a new CAX gene (OsCAX4) has been annotated. In the present study, we isolated the OsCAX4 gene and showed that its encoded protein possesses a unique transmembrane structure and is potentially involved in transporting not only Ca2+ but also Mn2+ and Cu2+. These six OsCAX genes differed in their mRNA expression pattern in roots of tolerant versus sensitive rice cultivars exposed to salt stress. For example, OsCAX4 showed abundant expression in IR29 (sensitive) upon prolonged salt stress. The mRNA expression profile of four putative vacuolar Ca2+-ATPases (OsACA4-7) was also examined. Under control conditions, the mRNA levels of OsACA4, OsACA5, and OsACA7 were relatively high and similar among IR29 and Pokkali. Upon salt stress, only OsACA4 showed first a decrease in its expression in Pokkali (tolerant), followed by a significant increase. Based on these results, a role of vacuolar Ca2+ transporter for salt tolerance in rice root was discussed.
Plant Physiology and Biochemistry Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand.
Nana
N
Yamada
Cattarin
C
Theerawitaya
Suriyan
S
Cha-um
Chalermpol
C
Kirdmanee
Teruhiro
T
Takabe
D000595:Amino Acid Sequence; D017920:Antiporters / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000096:biosynthesis / Q000235:genetics*; D027682:Cation Transport Proteins / Q000096:biosynthesis / Q000235:genetics*; D003001:Cloning, Molecular; D003300:Copper / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D008274:Magnesium / Q000378:metabolism; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D055051:Salt-Tolerant Plants / Q000378:metabolism*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000378:metabolism*
D017920:Antiporters; D027682:Cation Transport Proteins; D012333:RNA, Messenger; C027614:calcium-hydrogen antiporters; D012965:Sodium Chloride; D003300:Copper; D000252:Calcium-Transporting ATPases; D008274:Magnesium; D002118:Calcium