Dialysis method alters the expression of microRNA-33a and its target genes ABCA1, ABCG1 in THP-1 macrophages.
Atherosclerosis and accompanying cardiovascular disease are the first causes of mortality in patients undergoing maintenance hemodialysis. Anti-atherosclerotic effects of hemodiafiltration (HDF) have been reported. Our study aimed to investigate the effect of serum derived from a healthy group (n = 23), before and after hemodialysis (HD) therapy (n = 23), and before and after HDF therapy (n = 17) on the expression of microRNA-33a and its target genes adenosine triphosphate-binding cassette transporter A1,G1 (ABCA1, ABCG1) in THP-1 macrophages. Meanwhile, blood lipids and high-sensitivity C-reactive protein (hs-CRP) were measured in these groups. Our data showed that the expression of miRNA-33a was lower (P < 0.05) and ABCA1 and ABCG1 were higher (P < 0.05) in the healthy group than pre-HD and pre-HDF. miR-33a was significantly decreased (P < 0.05) but ABCA1, ABCG1 was significantly increased (P < 0.05) in post-HDF compared with pre-HDF, while these parameters in pre- and post- HD groups did not show any significant change (P > 0.05). High density lipoprotein cholesterol (HDL-C) was higher and hs-CRP was lower in the healthy group than pre-HD and pre-HDF groups. Moreover, a significant increase of HDL-C (P < 0.05) and decrease (P < 0.05) of hs-CRP was shown in post-HDF compared with pre-HDF, but HD appeared to have no significant change in these subjects. HDF therapy can downregulate miR-33a expression, and then result in ABCA1, ABCG1 upregulation and an increase in circulating HDL-C, leading to a possible anti-atherosclerosis effect to some extent.
Department of Nephrology, The First Affiliated Hospital of Chongqing Medical University, ChongQing, China.
Jian-Min
JM
Wang
Jia-Jun
JJ
Zhou
Qian
Q
Zheng
Hua
H
Gan
Hang
H
Wang
D064286:ATP Binding Cassette Transporter 1 / Q000235:genetics*; D000070998:ATP Binding Cassette Transporter, Subfamily G, Member 1; D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000368:Aged; D050197:Atherosclerosis / Q000517:prevention & control; D002097:C-Reactive Protein / Q000378:metabolism; D016022:Case-Control Studies; D002460:Cell Line; D008076:Cholesterol, HDL / Q000097:blood; D015536:Down-Regulation; D005260:Female; D017583:Hemodiafiltration / Q000379:methods; D006801:Humans; D008264:Macrophages / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D006435:Renal Dialysis / Q000379:methods*; D015854:Up-Regulation
C577836:ABCA1 protein, human; C419353:ABCG1 protein, human; D064286:ATP Binding Cassette Transporter 1; D000070998:ATP Binding Cassette Transporter, Subfamily G, Member 1; D018528:ATP-Binding Cassette Transporters; D008076:Cholesterol, HDL; C550416:MIRN33a microRNA, human; D035683:MicroRNAs; D002097:C-Reactive Protein
ABCA1; ABCG1; Atherosclerosis; Blood purification; Cholesterol; MicroRNA-33a