Lipoprotein CD0873 is a novel adhesin of Clostridium difficile.
Clostridium difficile is a cause of antibiotic-associated diarrhea and colitis, a healthcare-associated intestinal disease. Colonization of the gut is a critical step in the course of infection. The C. difficile lipoprotein CD0873 was identified as a putative adhesin through a bioinformatics approach. Surface exposure of CD0873 was confirmed and a CD0873 mutant was generated. The CD0873 mutant showed a significant reduction in adherence to Caco-2 cells and wild-type bacteria preincubated with anti-CD0873 antibodies showed significantly decreased adherence to Caco-2 cells. In addition, we demonstrated that purified recombinant CD0873 protein alone associates with Caco-2 cells. This is the first definitive identification of a C. difficile adhesin, which now allows work to devise improved measures for preventing and treating disease.
Biosciences, College of Life and Environmental Sciences, University of Exeter.
Andrea
A
Kovacs-Simon
Research Center, Novartis Vaccines and Diagnostics, Siena, Italy.
Rosanna
R
Leuzzi
Research Center, Novartis Vaccines and Diagnostics, Siena, Italy.
Magdalena
M
Kasendra
Clostridia Research Group, School of Molecular Medical Sciences, Centre for Biomolecular Sciences, University of Nottingham, United Kingdom.
Nigel
N
Minton
Biosciences, College of Life and Environmental Sciences, University of Exeter.
Richard W
RW
Titball
Biosciences, College of Life and Environmental Sciences, University of Exeter.
Stephen L
SL
Michell
D018829:Adhesins, Bacterial / Q000235:genetics / Q000378:metabolism*; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018938:Caco-2 Cells; D016360:Clostridioides difficile / Q000235:genetics / Q000502:physiology*; D019295:Computational Biology; D004847:Epithelial Cells / Q000382:microbiology*; D055786:Gene Knockout Techniques; D006801:Humans; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding
D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D008074:Lipoproteins; D008565:Membrane Proteins
ABC-type transporter; Bacteria; CD0873; Clostridium difficile; adhesion; lipoprotein