Pseudo-mannosylated DC-SIGN ligands as potential adjuvants for HIV vaccines.
The development of new and effective adjuvants may play a fundamental role in improving HIV vaccine efficacy. New classes of vaccine adjuvants activate innate immunity receptors, notably toll like receptors (TLRs). Adjuvants targeting the C-Type lectin receptor DC-SIGN may be alternative or complementary to adjuvants based on TRL activation. Herein we evaluate the ability of the glycomimetic DC-SIGN ligand Polyman 19 (PM 19) to modulate innate immune responses. Results showed that PM 19 alone, or in combination with TLR agonists, induces the expression of cytokines, β chemokines and co-stimulatory molecules that may, in turn, modulate adaptive immunity and exert anti-viral effects. These results indicate that the suitability of this compound as a vaccine adjuvant should be further evaluated.
Department of Biomedical and Clinical Sciences "L. Sacco", University of Milan, Via GB. Grassi 74, 20157 Milan, Italy. angela.berzi@unimi.it.
Angela
A
Berzi
Department of Chemistry, University of Milan, Via C.Golgi 19, 20133 Milan, Italy. norbert.varga@unibas.ch.
Norbert
N
Varga
Department of Chemistry, University of Milan, Via C.Golgi 19, 20133 Milan, Italy. sara.sattin@unimi.it.
Sara
S
Sattin
Unit of Obstetrics and Gynecology, Department of Biomedical and Clinical Sciences "L. Sacco", University of Milan, Via GB. Grassi 74, 20157 Milan, Italy. Antonazzo.Patrizio@hsacco.it.
Patrizio
P
Antonazzo
Department of Biomedical and Clinical Sciences "L. Sacco", University of Milan, Via GB. Grassi 74, 20157 Milan, Italy. mara.biasin@unimi.it.
Mara
M
Biasin
Unit of Obstetrics and Gynecology, Department of Biomedical and Clinical Sciences "L. Sacco", University of Milan, Via GB. Grassi 74, 20157 Milan, Italy. irene.cetin@unimi.it.
Irene
I
Cetin
Department of Biomedical and Clinical Sciences "L. Sacco", University of Milan, Via GB. Grassi 74, 20157 Milan, Italy. daria.trabattoni@unimi.it.
Daria
D
Trabattoni
Department of Chemistry, University of Milan, Via C.Golgi 19, 20133 Milan, Italy. anna.bernardi@unimi.it.
Anna
A
Bernardi
Department of Pathophysiology and Transplantation, University of Milan, Via F.lli VCervi 93, 20090 Milan, Italy. mario.clerici@unimi.it.
Mario
M
Clerici
D016915:AIDS Vaccines / Q000008:administration & dosage / Q000276:immunology*; D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism; D006801:Humans; D037181:Lectins, C-Type / Q000378:metabolism*; D008024:Ligands; D008351:Mannans / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
D016915:AIDS Vaccines; D000276:Adjuvants, Immunologic; D015815:Cell Adhesion Molecules; D016207:Cytokines; D037181:Lectins, C-Type; D008024:Ligands; D008351:Mannans; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C097323:polymannose