Triterpenoid saponins from Patrinia scabra.
OBJECTIVE
To study the chemical constituents and bioactivity of the roots of Patrinia scabra Bunge.


METHODS
The chemical constituents were isolated using various chromatographic methods, and the structures were elucidated on the basis of spectral analysis and chemical methods. In addition, cytotoxic activities toward HepG2 cells were tested by the MTT method.


RESULTS
A new triterpenoid saponin, 3-O-(4'-isovaleryl)-O-β-D-xylose-12,30-dihydroxy-oleanane-28,13-lactone-22-O- β-D-glucoside (1), along with two known triterpenoid saponins, acanthopanax saponin CP3 (2) and foetoside C (3), were isolated.


CONCLUSIONS
The aglycone of compound 1 was a new skeleton derivative of oleanolic acid. Compound 2 showed strong cytotoxicity to HePG2 cells (IC50 1.49 μmol·L(-1)).
Department of Natural Products Chemistry, China Pharmaceutical University, Nanjing 210009, China; Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University, Nanjing, 210009, China. Electronic address: fengsunlight@163.com.
Feng
F
Feng
Department of Natural Products Chemistry, China Pharmaceutical University, Nanjing 210009, China.
Xu
X
Xi-Yu
Department of Natural Products Chemistry, China Pharmaceutical University, Nanjing 210009, China.
Liu
L
Fu-Lei
Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, 210009, China; Key Laboratory of Drug Quality Control and Pharmacovigilance, China Pharmaceutical University, Ministry of Education, Nanjing 210009, China.
Liu
L
Wen-Yuan
Qingfeng Pharmaceutical Inc., Ganzhou 341000, China.
Xie
X
Ning
D002470:Cell Survival / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000633:toxicity; D056945:Hep G2 Cells; D006801:Humans; D015394:Molecular Structure; D032405:Patrinia / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D012503:Saponins / Q000737:chemistry* / Q000633:toxicity; D014315:Triterpenes / Q000737:chemistry* / Q000633:toxicity
D004365:Drugs, Chinese Herbal; D012503:Saponins; D014315:Triterpenes
Chemical constituents; Cytotoxicity; Patrinia scabra Bunge; R284; Triterpenoid saponins