[Quality standard of uygur medicine Medicago sativa seeds].
In this paper, microscopic identification method was adopted to observe the microscopic characters of ten batches of Medicago sativa seeds. And M. sativa seeds were identificated by TLC method in contrast to trigonelline and stachydrine hydrochloride. The impurities, moisture, ash, sour insoluble ash were detected based on Chinese Pharmacopoeia 2010 version (Vol I ). An HPLC method was also established for determination of trigonelline in the M. sativa seeds. The contents of impurities, moisture, ash, sour insoluble ash should not exceed 5%, 10%, 6%, and 2%, respectively. The content of trigonelline should be not less than 0.795 6 mg x g(-1). The experimental methods were accurate and reliable, and can be used as the quality control of the seeds of M. sativa.
Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, Urumqi 830011, China.
Wen-Huan
WH
Ding
Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, Urumqi 830011, China.
Hai-Yan
HY
Xu
Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, Urumqi 830011, China.
Dong-Dong
DD
Wang
Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, Urumqi 830011, China.
Jie
J
Li
Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, Urumqi 830011, China.
Shu-Ge
SG
Tian
D000470:Alkaloids / Q000032:analysis / Q000592:standards; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000592:standards; D000455:Medicago sativa / Q000737:chemistry* / Q000648:ultrastructure; D011786:Quality Control; D012639:Seeds / Q000737:chemistry / Q000648:ultrastructure
D000470:Alkaloids; D004365:Drugs, Chinese Herbal; C009560:trigonelline