Lethal effect of a hyperthermic CO₂ pneumoperitoneum on gastric cancer cells.
OBJECTIVE
We investigated the lethal effect of a hyperthermic CO₂ pneumoperitoneum on gastric cancer cells. This could form the theoretical basis for further studies of the feasibility and safety of inflating hyperthermic CO₂ in the abdominal cavity of gastric cancer patients during laparoscopy.


METHODS
An in vitro hyperthermic CO₂ pneumoperitoneum experimental model was built, where gastric cancer cell line SGC-7901 cells were grouped according to temperature. Cytotoxicity was detected using a cell counting kit; apoptosis was detected by Annexin V-FITC/PI flow cytometry and Hoechst 33342/PI fluorescent microscopy. Morphological alterations were observed by transmission electron microscopy. Invasion and migration were detected by a scratch test and by transwell migration, respectively.


RESULTS
Cytotoxicity assays showed that a hyperthermic CO₂ pneumoperitoneum significantly inhibited the proliferation of SGC-7901 cells (P<0.05); it also significantly induced apoptosis of SGC-7901 cells (P<0.05). Morphological observations showed that the cell membrane and nucleus had an apoptotic phenotype. The invasiveness and migration ability of the gastric cancer cells subjected to hyperthermic CO₂ were significantly reduced.


CONCLUSIONS
A hyperthermic CO₂ pneumoperitoneum had a lethal effect on gastric cancer SGC-7901 cells by inhibiting their invasion and migration, and by inducing apoptosis.
Department of Gastrointestinal Surgery, Subei People's Hospital of Jiangsu Province (Clinical Medical College of Yangzhou University), Yangzhou 225001, China.
Jiran
J
Zhang
Department of Gastrointestinal Surgery, Subei People's Hospital of Jiangsu Province (Clinical Medical College of Yangzhou University), Yangzhou 225001, China.
Dong
D
Tang
Department of Gastrointestinal Surgery, Subei People's Hospital of Jiangsu Province (Clinical Medical College of Yangzhou University), Yangzhou 225001, China.
Jie
J
Chen
Nanjing Medical University, Nanjing 210000, China.
Sen
S
Wang
Department of Gastrointestinal Surgery, Subei People's Hospital of Jiangsu Province (Clinical Medical College of Yangzhou University), Yangzhou 225001, China.
Jun
J
Gao
Department of Gastrointestinal Surgery, Subei People's Hospital of Jiangsu Province (Clinical Medical College of Yangzhou University), Yangzhou 225001, China.
Nianyuan
N
Ye
Department of Gastrointestinal Surgery, Subei People's Hospital of Jiangsu Province (Clinical Medical College of Yangzhou University), Yangzhou 225001, China. Electronic address: daorong666@163.com.
Daorong
D
Wang
D002245:Carbon Dioxide / Q000008:administration & dosage*; D006801:Humans; D006979:Hyperthermia, Induced; D011028:Pneumoperitoneum, Artificial; D013274:Stomach Neoplasms / Q000473:pathology* / Q000628:therapy; D014407:Tumor Cells, Cultured
D002245:Carbon Dioxide