MicroRNA-195 inhibits non-small cell lung cancer cell proliferation, migration and invasion by targeting MYB.
MicroRNA-195 (miR-195) has been implicated in several other cancers; however, its role in non-small cell lung cancer (NSCLC) remains unclear. In this study, we demonstrated that miR-195 was significantly down-regulated in NSCLC samples and cell lines compared with corresponding normal counterparts. In vitro and in vivo functional assays demonstrated that modulation of miR-195 expression affected NSCLC cell proliferation, migration and invasion. Using miRNA target prediction algorithms and reporter assays, we demonstrated that miR-195 suppressed the expression of MYB both at the mRNA and protein level, and was directly bound to the 3'untranslated region of MYB mRNA. Overexpression of MYB in NSCLC cells using an ectopic expression vector restored the decreased cell proliferation, migration and invasion effects induced by miR-195. Finally, we observed an inverse correlation between MYB and miR-195 in NSCLC. Taken together, our findings indicated that miR-195 functions as tumour suppressor in NSCLC, and the miR-195/MYB axis might represent a potential therapeutic target for NSCLC intervention.
Tumor Research Institute of Yunnan Province Tumor Hospital and The Third Affiliated Hospital of Kunming Medical University, PR China.
Zhou
Z
Yongchun
School of Public Health, Chinese University of Hong Kong, Hong Kong SAR, PR China.
Tian
T
Linwei
Tumor Research Institute of Yunnan Province Tumor Hospital and The Third Affiliated Hospital of Kunming Medical University, PR China.
Wang
W
Xicai
Department of Cardiothoracic Surgery, Yunnan Province Tumor Hospital and The Third Affiliated Hospital of Kunming Medical University, PR China.
Ye
Y
Lianhua
Department of Cardiothoracic Surgery, Yunnan Province Tumor Hospital and The Third Affiliated Hospital of Kunming Medical University, PR China.
Zhao
Z
Guangqiang
Institute of Molecular and Clinical Medicine, Kunming Medical University, PR China.
Yu
Y
Ming
Department of Cardiothoracic Surgery, Yunnan Province Tumor Hospital and The Third Affiliated Hospital of Kunming Medical University, PR China.
Li
L
Guanjian
Department of Cardiothoracic Surgery, Yunnan Province Tumor Hospital and The Third Affiliated Hospital of Kunming Medical University, PR China.
Lei
L
Yujie
Department of Cardiothoracic Surgery, Yunnan Province Tumor Hospital and The Third Affiliated Hospital of Kunming Medical University, PR China. Electronic address: huangyunchao2013@163.com.
Huang
H
Yunchao
D020413:3' Untranslated Regions; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D049109:Cell Proliferation; D015536:Down-Regulation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D020626:Oncogene Proteins v-myb / Q000235:genetics / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction
D020413:3' Untranslated Regions; C552722:MIRN195 microRNA, human; D035683:MicroRNAs; D020626:Oncogene Proteins v-myb
Invasion; MYB; Non-small cell lung cancer; Proliferation; miR-195