Thermosensitive micropatterned substrates.
We describe the design of micropatterned surfaces for single cell studies, based on photo-patterned thermoresponsive polymer brushes. Such surfaces allow for spatially controlled cell adhesion at 37°CC and thermal harvesting of the studied cells at T <32°CC.
Laboratoire Interdisciplinaire de Physique UMR 5588, Univ. Grenoble 1/CNRS, Grenoble, France.
Lionel
L
Bureau
Laboratoire Interdisciplinaire de Physique UMR 5588, Univ. Grenoble 1/CNRS, Grenoble, France.
Martial
M
Balland
D000180:Acrylic Resins / Q000494:pharmacology*; D000327:Adsorption; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects / Q000528:radiation effects; D055616:Microtechnology / Q000379:methods*; D058105:Polymerization / Q000187:drug effects / Q000528:radiation effects; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors; D014466:Ultraviolet Rays
D000180:Acrylic Resins; C052970:poly-N-isopropylacrylamide
Cell adhesion; Dynamic micropattern; Micropatterning; PNIPAM; Thermo-responsive