Analysis of the Rit subfamily GTPase-mediated signaling and neuronal differentiation and survival.
The Rit subfamily of GTPases is a founding branch within the Ras family of small G-proteins and preserves unique sequences in the G2 effector loop domain and the C-terminus. Rit proteins regulate a diversity of signal transduction pathways, some of which are similar to and others of which differ from the pathways that are regulated by other Ras family GTPases. Rit proteins have been demonstrated to be essential regulators in neuronal differentiation and survival. Here, we describe the materials and methods utilized to characterize cellular signaling for the Rit subfamily of G-proteins in neuronal differentiation and survival.
Cancer Center, University of Hawaii, Honolulu, HI, USA.
Geng-Xian
GX
Shi
Catherine N
CN
Kaminski
D000818:Animals; D001483:Base Sequence; D016923:Cell Death; D002454:Cell Differentiation; D002470:Cell Survival; D004591:Electrophoresis, Polyacrylamide Gel; D020868:Gene Silencing; D015151:Immunoblotting; D047468:Immunoprecipitation; D016501:Neurites / Q000378:metabolism; D009474:Neurons / Q000166:cytology*; D016716:PC12 Cells; D011494:Protein Kinases / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D015398:Signal Transduction; D014162:Transfection; D018631:ras Proteins / Q000378:metabolism*
D034741:RNA, Small Interfering; D011494:Protein Kinases; C486777:Rit protein, rat; D018631:ras Proteins