Suppressive effects of carotenoids on the antigen-induced degranulation in RBL-2H3 rat basophilic leukemia cells.
In this study, the anti-degranulation effects of fifteen carotenoids were evaluated using RBL-2H3 rat basophilic leukemia cell line as a mast cell model. Nine carotenoids, fucoxanthin, zeaxanthin, β-carotene, astaxanthin, 3-hydroxyechinenone, fucoxanthinol, lycopene, β-cryptoxanthin, and siphonaxanthin significantly suppressed antigen-induced mast cell degranulation. Under the same conditions, the cellular carotenoid contents were quantified using high performance liquid chromatography-photodiode array (HPLC-PDA). There was no correlation between the cellular carotenoid contents and their anti-degranulation activities. These results indicate that the differences in the anti-degranulation activities of carotenoids were not related to their uptake by the cells.
Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University.
Yuki
Y
Manabe
Takashi
T
Hirata
Tatsuya
T
Sugawara
D000818:Animals; D000941:Antigens / Q000276:immunology*; D001491:Basophils / Q000502:physiology*; D002338:Carotenoids / Q000494:pharmacology*; D015550:Cell Degranulation / Q000187:drug effects* / Q000276:immunology*; D003864:Depression, Chemical; D015471:Leukemia, Basophilic, Acute / Q000503:physiopathology*; D021962:Membrane Microdomains / Q000187:drug effects / Q000502:physiology; D051381:Rats; D014407:Tumor Cells, Cultured; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D000941:Antigens; D002338:Carotenoids; D001619:beta-N-Acetylhexosaminidases