Flavonoids from Lindera glauca Blume as low-density lipoprotein oxidation inhibitors.
In order to identify antioxidant flavonoids from Lindera glauca Blume, we performed phytochemical analysis of L. glauca Blume heartwood and isolated eight flavonoids - lindeglaucol (1), lindeglaucone (2), cilicicone B (3), tamarixetin 3-O-α-L-rhamnoside (4), procyanidin A2 (5), cinnamtannin B1 (6), cinnamtannin D1 (7), and procyanidin A1 (8) - through repeated column chromatography over silica gel (SiO₂), octadecyl silica gel (ODS) and Sephadex LH-20. The chemical structures of compounds 1-8 were elucidated from spectroscopic data (NMR, IR and MS). The low-density lipoprotein oxidation inhibitory activities of the isolated compounds were evaluated in vitro by using the thiobarbituric acid reactive substances assay. Compounds 5-8 exhibited high inhibition activity, comparable to the positive control butyl hydroxyl toluene. Compounds 2 and 3 were slightly less active, while 1 and 4 expressed low activity.
a The Graduate School of Biotechnology, Institute of Life Sciences & Resources, Kyung Hee University , Yongin 446-701 , Republic of Korea.
Gyu-Won
GW
Huh
Ji-Hae
JH
Park
Ji-Hyun
JH
Kang
Tae-Sook
TS
Jeong
Hee Cheol
HC
Kang
Nam-In
NI
Baek
D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry*; D027443:Lindera / Q000737:chemistry*; D008077:Lipoproteins, LDL / Q000032:analysis / Q000737:chemistry*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010084:Oxidation-Reduction; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis / Q000737:chemistry
D000975:Antioxidants; D005419:Flavonoids; D008077:Lipoproteins, LDL; D017392:Thiobarbituric Acid Reactive Substances; C019455:low density lipoprotein inhibitor
LDL-oxidation; Lindera glauca; NMR; flavonoid