Salt-assisted LLE combined with field-amplified sample stacking in CE for improved determination of beta blocker drugs in human urine.
BACKGROUND
A simple and sensitive CE method was developed and validated for the analysis of some beta blockers in human urine.


METHODS
In this study, salting-out assisted LLE combined with field-ampliﬁed sample stacking method was employed for biological sample clean-up and sensitivity enhancement in CE.


RESULTS
Under the optimal conditions good linearity (r(2) ≥0.998) was obtained, within 0.025-1 µg/ml for propranolol and metoprolol, and within 0.05-1 µg/ml for carvedilol in urine samples. LODs and LLOQs ranged from 0.005 to 0.015 µg/ml, and from 0.025 to 0.05 µg/ml, respectively. The RSDs of intra- and inter-day analysis of examined compounds were less than 4.0%. The recoveries were in the range of 98-119%.


CONCLUSIONS
The validated method is successfully applied to determine propranolol, metoprolol and carvedilol in human urine samples obtained from the patients who received these drugs.
Biomedical Analysis Lab, Liver & Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Rana
R
Fazeli-Bakhtiyari
Mohammad Hossein
MH
Sorouraddin
Mir Ali
MA
Farajzadeh
Mohammad Hossein
MH
Somi
Abolghasem
A
Jouyban
D000319:Adrenergic beta-Antagonists / Q000737:chemistry / Q000302:isolation & purification* / Q000652:urine*; D053000:Analytic Sample Preparation Methods / Q000379:methods*; D002138:Calibration; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012492:Salts / Q000737:chemistry*; D016482:Urinalysis / Q000379:methods*
D000319:Adrenergic beta-Antagonists; D012492:Salts