HLA-G1 increases the radiosensitivity of human tumoral cells.
Different molecules regulate the response of tumoral tissues to ionizing radiation. The objective of this work was to determine if HLA-G1 expression modulates the radiosensitivity of human tumoral cell lines. To this end, human melanoma M8 and human erythroleukemia K562 cell lines, with their correspondent HLA-G1 negative and positive variants, were gamma irradiated and the survival frequency was determined by clonogenic assay. The survival fraction of HLA-G1 expressing cells was around 60% of HLA-G1 negative cells. The generation of acidic vesicular organelles was higher in HLA-G1 positive cells. Apoptosis levels showed statistically significant differences only in K562 cells, whereas the variation in G2/M cycle progression was only significant in M8 cells. In addition, irradiation diminished cell-surface HLA-G1 and increased soluble HLA-G1 levels. Soluble HLA-G1 has no influence on cell survival in any cell line. In summary, we could demonstrate that HLA-G1 confers higher radiosensitivity to HLA-G1 expressing cells.
Radiopathology Laboratory, Autoridad Regulatoria Nuclear (ARN), Buenos Aires, Argentina; Toxicology Laboratory, Universidad Nacional del Sur (UNS), Bahía Blanca, Argentina.
Cristina E
CE
Gallegos
Radiopathology Laboratory, Autoridad Regulatoria Nuclear (ARN), Buenos Aires, Argentina. Electronic address: smichelin@arn.gob.ar.
Severino
S
Michelin
Radiopathology Laboratory, Autoridad Regulatoria Nuclear (ARN), Buenos Aires, Argentina.
Sofía
S
Baffa Trasci
Radiopathology Laboratory, Autoridad Regulatoria Nuclear (ARN), Buenos Aires, Argentina.
Elizabeth Aballay
EA
Lobos
Radiopathology Laboratory, Autoridad Regulatoria Nuclear (ARN), Buenos Aires, Argentina.
Diana
D
Dubner
Commissariat a l'Energie Atomique et aux Energies Alternatives (CEA), Institute of Emerging Diseases and Innovative Therapies (iMETI), Research Division in Hematology and Immunology (SRHI), Paris, France; University Paris Diderot, Sorbonne Paris Cité, UMR E-5 Institut Universitaire d'Hematologie, Saint-Louis Hospital, Paris, France.
Edgardo D
ED
Carosella
D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002470:Cell Survival; D005720:Gamma Rays; D059951:HLA-G Antigens / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D020014:K562 Cells; D004915:Leukemia, Erythroblastic, Acute / Q000276:immunology / Q000532:radiotherapy*; D008545:Melanoma / Q000276:immunology / Q000532:radiotherapy*; D011836:Radiation Tolerance
D014408:Biomarkers, Tumor; D059951:HLA-G Antigens
Erythroleukemia cells; HLA-G1; Ionizing radiation; Melanoma cells; Radiosensitivity