Mannose-binding lectin-2 and ficolin-2 gene polymorphisms and clinical risk factors for acute rejection in kidney transplantation.
BACKGROUND
There is growing evidence that the lectin pathway is significantly associated with acute rejection. Rare studies associated both gene polymorphisms of MBL2 and FCN2 with acute rejection after kidney transplantation. The aim of the present study was to investigate the role of the lectin gene profile and clinical risk factors such as PRA level on acute rejection in kidney transplant recipients.


METHODS
We prospectively analyzed 157 kidney transplant recipients with and without acute rejection. A total of 6 well-known functional single-nucleotide polymorphisms in the MBL2 gene and 5 in the FCN2 gene of the recipients were determined by gene sequencing. MBL2 and FCN2 genotypic variants were analyzed for association with the incidence of acute rejection within the first year after kidney transplantation.


RESULTS
After adjusting for variables of P<0.2, we found the differences in the incidence of acute rejection were only according to panel-reactive antibodies (odds ratios (OR) = 6.468, 95% confidence intervals (CI)= 2.017-20.740, P = 0.002) and the HH genotypes of MBL2 promoter -550 (OR = 2.448, 95%CI = 1.026-5.839, P = 0.044).


CONCLUSIONS
Panel-reactive antibodies and the HH genotypes of MBL2 promoter -550 have significant impacts on the risk of developing acute rejection after kidney transplantation.
Nursing Department, the Third Xiangya Hospital, Central South University, Changsha 410013, Hunan, China.
Xiaoxia
X
Wu
Department of Transplant Surgery, the Third Xiangya Hospital, Central South University, Changsha 410013, Hunan, China. Electronic address: 13548685542@163.com.
Qiquan
Q
Wan
Department of Transplant Surgery, the Third Xiangya Hospital, Central South University, Changsha 410013, Hunan, China.
Qifa
Q
Ye
Department of Clinical Laboratory of Microbiology, the Third Xiangya Hospital, Central South University, Changsha 410013, Hunan, China.
Jiandang
J
Zhou
D000208:Acute Disease; D000328:Adult; D005260:Female; D005500:Follow-Up Studies; D005838:Genotype; D006084:Graft Rejection / Q000235:genetics*; D006801:Humans; D016030:Kidney Transplantation; D037102:Lectins / Q000235:genetics*; D008297:Male; D037601:Mannose-Binding Lectin / Q000235:genetics*; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D012307:Risk Factors; D000097604:Ficolins
D037102:Lectins; D037601:Mannose-Binding Lectin; D000097604:Ficolins; C445619:MBL2 protein, human
Acute rejection; Ficolin-2; Gene polymorphism; Kidney transplantation; Mannose-binding lectin; Panel-reactive antibodies