Diverse effect of different odor stimuli on behavior and Fos protein production in the olfactory system neurogenic region of adult rats.
Previously it has been demonstrated that processes of postnatal neurogenesis in the olfactory system neurogenic region-the subventricular zone (SVZ), rostral migratory stream (RMS), and olfactory bulb (OB) can be significantly altered by different factors of an environment. However, the mechanisms involved in regulation of neurogenesis by exogenous factors in the olfactory system remain unclear. The purpose of the present study was to contribute to the understanding of these mechanisms by immunohistochemical assessment of Fos protein induction in areas of adult neurogenesis. To evaluate the coordinate activation of Fos production in neurons of the olfactory system neurogenic region, a brief exposure to artificial odor (eau de Cologne) or naturalistic odor (cat odor) has been used in alert rats. Our results revealed that the effects of these odors are easily distinguishable at both the behavioral and the morphological level. Cat odor induced greater changes in anxiety level, and produced typical pattern of Fos activation in the accessory olfactory bulb (AOB), a brain region associated with defensive behavior. An important finding is, that next to distinct Fos expression in the OB and the AOB, Fos positive cells have been found also within the SVZ/RMS of the odor stimulated rats. Interestingly, Fos expression in the RMS was detected only after exposure to artificial odor stimulus. These results provide new evidence that some SVZ/RMS cells have complete prerequisites necessary for the Fos signal transduction cascade.
Institute of Neurobiology, Slovak Academy of Sciences, Šoltésovej 4, 04001, Košice, Slovak Republic. Electronic address: lievajova@saske.sk.
Kamila
K
Fabianová
Institute of Neurobiology, Slovak Academy of Sciences, Šoltésovej 4, 04001, Košice, Slovak Republic.
Marcela
M
Martončíková
Institute of Animal Physiology, Slovak Academy of Science, Šoltésovej 4, 04001, Košice, Slovak Republic.
Dušan
D
Fabian
Institute of Neurobiology, Slovak Academy of Sciences, Šoltésovej 4, 04001, Košice, Slovak Republic.
Juraj
J
Blaško
Institute of Neurobiology, Slovak Academy of Sciences, Šoltésovej 4, 04001, Košice, Slovak Republic.
Enikő
E
Račeková
D000704:Analysis of Variance; D000818:Animals; D002415:Cats; D005106:Exploratory Behavior / Q000502:physiology*; D020547:Lateral Ventricles / Q000166:cytology / Q000378:metabolism; D008124:Locomotion / Q000502:physiology*; D008297:Male; D009249:NADP / Q000378:metabolism; D055495:Neurogenesis / Q000502:physiology*; D009812:Odorants; D009833:Olfactory Pathways / Q000166:cytology* / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D016760:Proto-Oncogene Proteins c-fos; D009249:NADP
Adult neurogenesis; Fos protein; anxiety; artificial odor; cat odor