Guanine nucleotide-binding protein subunit beta-2-like 1, a new Annexin A7 interacting protein.
We report for the first time that Guanine nucleotide-binding protein subunit beta-2-like 1 (RACK1) formed a complex with Annexin A7. Hca-F and Hca-P are a pair of syngeneic mouse hepatocarcinoma cell lines established and maintained in our laboratory. Our previous study showed that both Annexin A7 and RACK1 were expressed higher in Hca-F (lymph node metastasis >70%) than Hca-P (lymph node metastasis <30%). Suppression of Annexin A7 expression in Hca-F cells induced decreased migration and invasion ability. In this study, knockdown of RACK1 by RNA interference (RNAi) had the same impact on metastasis potential of Hca-F cells as Annexin A7 down-regulation. Furthermore, by co-immunoprecipitation and double immunofluorescence confocal imaging, we found that RACK1 was in complex with Annexin A7 in control cells, but not in the RACK1-down-regulated cells, indicating the abolishment of RACK1-Annexin A7 interaction in Hca-F cells by RACK1 RNAi. Taken together, these results suggest that RACK1-Annexin A7 interaction may be one of the means by which RACK1 and Annexin A7 influence the metastasis potential of mouse hepatocarcinoma cells in vitro.
Key Laboratory of Tumor Metastasis of Liaoning Province, Department of Pathology, Dalian Medical University, Dalian 116044, China.
Yue
Y
Du
Key Laboratory of Tumor Metastasis of Liaoning Province, Department of Pathology, Dalian Medical University, Dalian 116044, China.
Jinyi
J
Meng
Key Laboratory of Tumor Metastasis of Liaoning Province, Department of Pathology, Dalian Medical University, Dalian 116044, China.
Yuhong
Y
Huang
Key Laboratory of Tumor Metastasis of Liaoning Province, Department of Pathology, Dalian Medical University, Dalian 116044, China.
Jun
J
Wu
Key Laboratory of Tumor Metastasis of Liaoning Province, Department of Pathology, Dalian Medical University, Dalian 116044, China.
Bo
B
Wang
Key Laboratory of Tumor Metastasis of Liaoning Province, Department of Pathology, Dalian Medical University, Dalian 116044, China.
Mohammed M
MM
Ibrahim
Key Laboratory of Tumor Metastasis of Liaoning Province, Department of Pathology, Dalian Medical University, Dalian 116044, China. Electronic address: jianwutdlmedu@163.com.
Jianwu
J
Tang
D000818:Animals; D017310:Annexin A7 / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008207:Lymphatic Metastasis; D051379:Mice; D018613:Microscopy, Confocal; D009479:Neuropeptides / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D034622:RNA Interference; D000075370:Receptors for Activated C Kinase; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017310:Annexin A7; D009479:Neuropeptides; C090294:RACK1 protein, mouse; D000075370:Receptors for Activated C Kinase
Annexin A7 (ANXA7); Guanine nucleotide-binding protein subunit beta-2-like 1 (Gnb2l1); Lymph node metastasis (LNM); Receptor of activated protein kinase C 1 (RACK1)