Use of in vitro bioassays for assessing botanicals.
In vitro bio assays can play a vital role in evaluating botanicals ranging from comparative screening, interaction studies, bio-activity guided fractionation, biological characterization, assisting in stability studies to studying mechanism of actions. In this review, we present some challenges and common pitfalls of using bioassays for assessing botanicals including guidance to overcome them. The overall objective of this review is to improve the success of botanicals products by incorporating robust bioassays during various stages of research and development.
R&D Centre, Natural Remedies Pvt. Ltd., Plot 5B, Veerasandra Industrial Area, Electronic City PO, Hosur Road, Bangalore 560 100, Karnataka, India. Electronic address: amit@naturalremedy.com.
Amit
A
Agarwal
R&D Centre, Natural Remedies Pvt. Ltd., Plot 5B, Veerasandra Industrial Area, Electronic City PO, Hosur Road, Bangalore 560 100, Karnataka, India.
Prashanth
P
D'Souza
R&D Centre, Natural Remedies Pvt. Ltd., Plot 5B, Veerasandra Industrial Area, Electronic City PO, Hosur Road, Bangalore 560 100, Karnataka, India.
T Sudhakar
TS
Johnson
R&D Centre, Natural Remedies Pvt. Ltd., Plot 5B, Veerasandra Industrial Area, Electronic City PO, Hosur Road, Bangalore 560 100, Karnataka, India.
Shekhar M
SM
Dethe
R&D Centre, Natural Remedies Pvt. Ltd., Plot 5B, Veerasandra Industrial Area, Electronic City PO, Hosur Road, Bangalore 560 100, Karnataka, India.
Cv
C
Chandrasekaran
D001681:Biological Assay / Q000379:methods*; D001682:Biological Availability; D006801:Humans; D010944:Plants / Q000737:chemistry* / Q000378:metabolism; D011786:Quality Control; D015203:Reproducibility of Results