Conventional transmission electron microscopy.
Researchers have used transmission electron microscopy (TEM) to make contributions to cell biology for well over 50 years, and TEM continues to be an important technology in our field. We briefly present for the neophyte the components of a TEM-based study, beginning with sample preparation through imaging of the samples. We point out the limitations of TEM and issues to be considered during experimental design. Advanced electron microscopy techniques are listed as well. Finally, we point potential new users of TEM to resources to help launch their project.
Molecular, Cellular, and Developmental Biology, University of Colorado at Boulder, Boulder, CO 80309 Boulder Laboratory for 3D Electron Microscopy of Cells, University of Colorado at Boulder, Boulder, CO 80309.
Mark
M
Winey
Janet B
JB
Meehl
Eileen T
ET
O'Toole
Thomas H
TH
Giddings
D000818:Animals; D003585:Cell Biology; D002477:Cells / Q000166:cytology*; D005404:Fixatives; D006801:Humans; D046529:Microscopy, Electron, Transmission / Q000295:instrumentation* / Q000379:methods*; D008867:Microtomy / Q000379:methods; D009993:Osmium Tetroxide; D013048:Specimen Handling; D013194:Staining and Labeling / Q000379:methods
D005404:Fixatives; D009993:Osmium Tetroxide