Matrix metalloproteinases and E-cadherin immunoreactivity in different basal cell carcinoma histological types.
The immunohistochemical staining of matrix metalloproteinases (MMPs) and E-cadherin in tumor epithelial and stromal cells was analyzed in a group of solid, superficial spreading and cystic tumors and in a group of morpheaform and recurrent basal cell carcinomas (BCC) in order to determine whether any of these factors possibly contribute to tumor therapy resistance. Tumor tissues of 64 patients were obtained by complete excisional or curettage biopsy of BCC and these were immunohistochemically stained for MMP-1, MMP-2, MMP-9, MMP-13 and E-cadherin. In the morpheaform and recurrent BCC, MMP-9 expression significantly increased in the stroma, while E-cadherin expression was negative in epithelial cells. Odds ratio for development of morpheaform and recurrent BCC was 6.2 for positive MMP-1 immunostaining in epithelial tumor cells, 5.8 for positive MMP-9 immunostaining in tumor stroma, 3.2 for positive MMP-13 immunostaining in tumor stroma, and 4.5 for negative E-cadherin in epithelial tumor cells. Our results suggest that MMP-1 immunostaining in tumor cells, MMP-9 expression in stromal cells, and absence of E-cadherin expression are associated with morpheaform and recurrent BCC.
Vanjaka Rogošić Private Dermatovenereology Clinic, Split, Croatia.
Lucija
L
Vanjaka-Rogošić
Clinical Department of Dermatology and Venereology, Split University Hospital Center, Split, Croatia.
Neira
N
Puizina-Ivić
Clinical Department of Dermatology and Venereology, Split University Hospital Center, Split, Croatia.
Lina
L
Mirić
Clinical Department of Ophthalmology, Split University Hospital Center, Split, Croatia.
Veljko
V
Rogošić
Clinical Department of Pathology, Forensic Medicine and Cytology, Split University Hospital Center, Split, Croatia.
Ivana
I
Kuzmić-Prusac
Department of Anatomy, Histology and Embryology, School of Medicine, University of Split, Split, Croatia.
Mirna Saraga
MS
Babić
Clinical Department of Dermatology and Venereology, Split University Hospital Center, Split, Croatia.
Dubravka
D
Vuković
Department of Anatomy, Histology and Embryology, School of Medicine, University of Split, Split, Croatia. Electronic address: snjezana.mardesic.brakus@mefst.hr.
Snježana
S
Mardešić
D015820:Cadherins / Q000378:metabolism*; D002280:Basal Cell Carcinoma / Q000201:enzymology / Q000378:metabolism / Q000503:physiopathology*; D006801:Humans; D007150:Immunohistochemistry; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D015999:Multivariate Analysis
D015820:Cadherins; D020782:Matrix Metalloproteinases
Basal cell carcinoma; E-cadherin; MMP-1; MMP-13; MMP-2; MMP-9