Kinetics of dengue non-structural protein 1 antigen and IgM and IgA antibodies in capillary blood samples from confirmed dengue patients.
Large-scale epidemiological surveillance of dengue in the field and dengue patient management require simple methods for sample collection, storage, and transportation as well as effective diagnostic tools. We evaluated the kinetics of three biological markers of dengue infection-non-structural protein 1 (NS1) antigen, immunoglobulin M (IgM), and IgA-in sequential capillary blood samples collected from fingertips of confirmed dengue patients. The overall sensitivities and specificities of the tests were 96% and 100%, respectively, for NS1, 58.1% and 100%, respectively, for IgM, and 33% and 100%, respectively, for IgA. During the acute phase of the disease, NS1 was the best marker of dengue infection, with a sensitivity of 98.7%, whereas from day 5, all three markers exhibited relevant levels of sensitivity. This first descriptive study of the kinetics of biological markers of dengue in capillary blood samples confirms the usefulness of this biological compartment for dengue diagnosis and argues for its exploitation in community-level and remote settings.
Laboratoire de Virologie, Centre National de Référence des Arbovirus, Laboratoire Associé, Région Antilles Guyane, Institut Pasteur de la Guyane, Cayenne, French Guiana; Far East Medical Vietnam Limited, Ho Chi Minh City, Vietnam; Pasteur Institute of Ho Chi Minh City, Ho Chi Minh City, Vietnam; Laboratoire des Interactions Virus-Hôtes, Institut Pasteur de la Guyane, Cayenne, French Guiana; Centre d'Epidémiologie et de Santé Publique des Armées, Marseille, France; Unité Mixte de Recherche Scientifique 872, Pôle 4, Equipe 16, Institut National de la Santé et de la Recherche Médicale, Centre de Recherches Biomédicales des Cordeliers, Paris, France.
Séverine
S
Matheus
Thai Binh
TB
Pham
Bhetty
B
Labeau
Vu Thi Que
VT
Huong
Vincent
V
Lacoste
Xavier
X
Deparis
Vincent
V
Marechal
D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D015415:Biomarkers / Q000097:blood; D001800:Blood Specimen Collection / Q000379:methods; D002196:Capillaries; D003715:Dengue / Q000097:blood* / Q000276:immunology; D003716:Dengue Virus / Q000276:immunology*; D018450:Disease Progression; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology*; D008137:Longitudinal Studies; D008875:Middle Aged; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D013997:Time Factors; D014744:Vietnam; D017361:Viral Nonstructural Proteins / Q000276:immunology*; D055815:Young Adult
D000914:Antibodies, Viral; D000956:Antigens, Viral; D015415:Biomarkers; D007070:Immunoglobulin A; D007075:Immunoglobulin M; D017361:Viral Nonstructural Proteins