[Optimization of supercritical fluid extraction of bioactive components in Ligusticum chuanxiong by orthogonal array design].
With the yields of ferulic acid, coniferylferulate, Z-ligustilide, senkyunolide A, butylidenephthalide, butylphthalide, senkyunolide I, senkyunolide H, riligustilide, levistolide A, and total pharmacologically active ingredient as evaluation indexes, the extraction of Ligusticum chuanxiong by supercritical fluid technology was investigated through an orthogonal experiment L9 (3(4)). Four factors, namely temperature, pressure, flow rate of carbon dioxide, co-solvent concentration of the supercritical fluid, were investigated and optimized. Under the optimized conditions, namely 65 degrees C of temperature, 35 MPa of pressure, 1 L x min(-1) of CO2 flow rate, 8% of co-solvent concetration, supercritical fluid extraction could achieve a better yield than the conventional reflux extraction using methanol. And the supercritical fluid extraction process was validated to be stable and reliable.
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China. hlc0614@163.com
Li-Cui
LC
Hu
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
Xun
X
Wu
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
Xue-Dong
XD
Yang
D001572:Benzofurans / Q000032:analysis / Q000302:isolation & purification; D025924:Chromatography, Supercritical Fluid / Q000295:instrumentation / Q000379:methods*; D003373:Coumaric Acids / Q000032:analysis / Q000302:isolation & purification; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000302:isolation & purification*; D030004:Ligusticum / Q000737:chemistry*
D001572:Benzofurans; D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; C576743:senkyunolide I; C004999:ferulic acid