Cryptosporidium parvum GP60 subtypes in dairy cattle from Buenos Aires, Argentina.
Cryptosporidium parvum from 73 dairy calves less than two months old from Buenos Aires province (Argentina) were molecularly characterized using sequence analysis of the GP60 gene. Seventy-five sequences were obtained, and seven different subtypes were identified, all belonging to the IIa subtype family. The most common subtypes were IIaA20G1R1 (27/75), IIaA22G1R1 (16/75), and IIaA18G1R1 (13/75). Subtypes IIaA21G1R1, IIaA23G1R1, IIaA16G1R1 and IIaA19G1R1 were found sporadically. Two samples contained mixed infections with IIaA21G1R1 and IIaA22G1R1. A significant association was found between subtypes and geographic location, whereas there was no relation between subtypes and presence of diarrhea. Three of the subtypes found in this study (IIaA16G1R1, IIaA18G1R1, and IIaA19G1R1) were previously identified in humans. These findings suggest that cattle could play an important role in the transmission of cryptosporidiosis to humans in Buenos Aires province.
Cátedra de Microbiología y Parasitología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, 60 y 120, La Plata 1900, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. Electronic address: vdelcoco@aetos.med.unlp.edu.ar.
Valeria F
VF
Del Coco
Cátedra de Microbiología y Parasitología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, 60 y 120, La Plata 1900, Argentina; Comisión de Investigaciones Científicas de la Provincia de Buenos Aires, Argentina.
María A
MA
Córdoba
Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Argentina.
Gladys
G
Bilbao
Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Argentina.
Aldana Pinto
AP
de Almeida Castro
Cátedra de Microbiología y Parasitología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, 60 y 120, La Plata 1900, Argentina.
Juan A
JA
Basualdo
Environmental Microbial and Food Safety Laboratory, Agricultural Research Service, United States Department of Agriculture, Building 173, BARC-East, Beltsville, MD 20705, USA.
Ronald
R
Fayer
Environmental Microbial and Food Safety Laboratory, Agricultural Research Service, United States Department of Agriculture, Building 173, BARC-East, Beltsville, MD 20705, USA.
Mónica
M
Santín
D000818:Animals; D000833:Animals, Suckling; D001118:Argentina; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D016009:Chi-Square Distribution; D003457:Cryptosporidiosis / Q000469:parasitology*; D016785:Cryptosporidium parvum / Q000145:classification / Q000235:genetics*; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D003967:Diarrhea / Q000469:parasitology / Q000662:veterinary*; D005243:Feces / Q000469:parasitology; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D010270:Parasite Egg Count / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA; D015047:Zoonoses / Q000469:parasitology*
D016054:DNA, Protozoan
Argentina; Cattle; Cryptosporidium parvum; GP60; Molecular epidemiology; Subtypes