A "three-in-one" sample preparation method for simultaneous determination of B-group water-soluble vitamins in infant formula using VitaFast(®) kits.
VitaFast(®) test kits designed for the microbiological assay in microtiter plate format can be applied to quantitative determination of B-group water-soluble vitamins such as vitamin B12, folic acid and biotin, et al. Compared to traditional microbiological methods, VitaFast(®) kits significantly reduce sample processing time and provide greater reliability, higher productivity and better accuracy. Recently, simultaneous determination of vitamin B12, folic acid and biotin in one sample is urgently required when evaluating the quality of infant formulae in our practical work. However, the present sample preparation protocols which are developed for individual test systems, are incompatible with simultaneous determination of several analytes. To solve this problem, a novel "three-in-one" sample preparation method is herein developed for simultaneous determination of B-group water-soluble vitamins using VitaFast(®) kits. The performance of this novel "three-in-one" sample preparation method was systematically evaluated through comparing with individual sample preparation protocols. The experimental results of the assays which employed "three-in-one" sample preparation method were in good agreement with those obtained from conventional VitaFast(®) extraction methods, indicating that the proposed "three-in-one" sample preparation method is applicable to the present three VitaFast(®) vitamin test systems, thus offering a promising alternative for the three independent sample preparation methods. The proposed new sample preparation method will significantly improve the efficiency of infant formulae inspection.
Key Laboratory of Sensing Technology and Biomedical Instruments (Guangdong Province), School of Engineering, Sun Yat-Sen University, Guangzhou 510275, China; Shenzhen Key Research Laboratory of Detection Technology R&D on Food Safety, Food Inspection Center of CIQ-Shenzhen, Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518045, China.
Heng
H
Zhang
Shenzhen Key Research Laboratory of Detection Technology R&D on Food Safety, Food Inspection Center of CIQ-Shenzhen, Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518045, China.
Fang
F
Lan
Key Laboratory of Sensing Technology and Biomedical Instruments (Guangdong Province), School of Engineering, Sun Yat-Sen University, Guangzhou 510275, China.
Yupeng
Y
Shi
Shenzhen Key Research Laboratory of Detection Technology R&D on Food Safety, Food Inspection Center of CIQ-Shenzhen, Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518045, China.
Zhi-Gang
ZG
Wan
Shenzhen Key Research Laboratory of Detection Technology R&D on Food Safety, Food Inspection Center of CIQ-Shenzhen, Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518045, China.
Zhen-Feng
ZF
Yue
Shenzhen Key Research Laboratory of Detection Technology R&D on Food Safety, Food Inspection Center of CIQ-Shenzhen, Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518045, China.
Fang
F
Fan
Shenzhen Key Research Laboratory of Detection Technology R&D on Food Safety, Food Inspection Center of CIQ-Shenzhen, Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518045, China.
Yan-Kui
YK
Lin
Shenzhen Key Research Laboratory of Detection Technology R&D on Food Safety, Food Inspection Center of CIQ-Shenzhen, Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518045, China.
Mu-Jin
MJ
Tang
Shenzhen Key Research Laboratory of Detection Technology R&D on Food Safety, Food Inspection Center of CIQ-Shenzhen, Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518045, China. Electronic address: jz_lv@yahoo.com.cn.
Jing-Zhang
JZ
Lv
Key Laboratory of Sensing Technology and Biomedical Instruments (Guangdong Province), School of Engineering, Sun Yat-Sen University, Guangzhou 510275, China. Electronic address: xiaotan@mail.sysu.edu.cn.
Tan
T
Xiao
Key Laboratory of Sensing Technology and Biomedical Instruments (Guangdong Province), School of Engineering, Sun Yat-Sen University, Guangzhou 510275, China. Electronic address: yichq@mail.sysu.edu.cn.
Changqing
C
Yi
D053000:Analytic Sample Preparation Methods / Q000295:instrumentation / Q000379:methods*; D015374:Biosensing Techniques / Q000379:methods; D005492:Folic Acid / Q000032:analysis* / Q000302:isolation & purification* / Q000378:metabolism; D041943:Infant Formula / Q000737:chemistry*; D007778:Lactobacillus / Q000378:metabolism; D011933:Reagent Kits, Diagnostic; D012995:Solubility; D014805:Vitamin B 12 / Q000032:analysis* / Q000302:isolation & purification* / Q000378:metabolism
D011933:Reagent Kits, Diagnostic; D005492:Folic Acid; D014805:Vitamin B 12
Infant formula; Microbiological assay; Sample preparation; Water-soluble vitamins