Association of the polymorphisms of MMP-9 and TIMP-3 genes with thoracic aortic dissection in Chinese Han population.
OBJECTIVE
Thoracic aortic dissection (TAD) is the most common life-threatening disorder, and a shifted balance of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) is involved in TAD pathogenesis. The aim of this study was to evaluate the association of 4 single-nucleotide polymorphisms (SNPs) in MMP-9 and TIMP-3 genes with TAD risk in Chinese Han population.


METHODS
A total of 206 Chinese patients with TAD and 180 controls were included in this study. Four SNPs (rs3918249, rs2274756, rs9609643 and rs8136803) were genotyped using high-throughput MALDI-TOF mass spectrometry. Allele and genotype association analyses were conducted using PLINK.


RESULTS
All the 4 SNPs resulted in Hardy-Weinberg equilibrium in patients and controls. The G allele frequency for the MMP-9 SNP rs2274756 was significantly higher in female TAD patients than in female controls (P=0.0099). Moreover, after adjusting for traditional cardiovascular risk factors (sex, age, hypertension, dyslipidemia, diabetes and smoking habit), the rs2274756 polymorphism (odds ratio: 0.30; 95% confidence interval: 0.11 to 0.79, P=0.015) resulted in an independent susceptibility factor for TAD in females. No associations were found between the other SNPs and TAD.


CONCLUSIONS
The results provide strong evidence for an association between MMP-9 SNP rs2274756 and female TAD risk in Chinese Han population.
1] Department of Hepatobiliary Surgery, the First Affiliated Hospital of Medical College, Xi-an Jiaotong University, Xi-an 710000, China [2] Department of Cardiovascular Surgery, Beijing An Zhen Hospital, Capital Medical University, Beijing 100029, China.
Xiao-long
XL
Wang
1] Department of Cardiovascular Surgery, Beijing An Zhen Hospital, Capital Medical University, Beijing 100029, China [2] The Key Laboratory of Remodeling-related Cardiovascular Diseases, Capital Medical University, Ministry of Education, Beijing 100029, China.
Ou
O
Liu
Department of Cardiovascular Surgery, Beijing An Zhen Hospital, Capital Medical University, Beijing 100029, China.
Yan-wen
YW
Qin
Department of Cardiovascular Surgery, Beijing An Zhen Hospital, Capital Medical University, Beijing 100029, China.
Hong-jia
HJ
Zhang
1] Department of Hepatobiliary Surgery, the First Affiliated Hospital of Medical College, Xi-an Jiaotong University, Xi-an 710000, China [2] Department of Cardiovascular Surgery, Beijing An Zhen Hospital, Capital Medical University, Beijing 100029, China.
Yi
Y
Lv
D000293:Adolescent; D000328:Adult; D000368:Aged; D000784:Aortic Dissection / Q000175:diagnosis / Q000201:enzymology / Q000208:ethnology / Q000235:genetics*; D017545:Aortic Aneurysm, Thoracic / Q000175:diagnosis / Q000201:enzymology / Q000208:ethnology / Q000235:genetics*; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D008875:Middle Aged; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D012737:Sex Factors; D019717:Tissue Inhibitor of Metalloproteinase-3 / Q000235:genetics*; D055815:Young Adult
C088390:TIMP3 protein, human; D019717:Tissue Inhibitor of Metalloproteinase-3; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9