Construction and immunogenicity of DNA vaccines encoding fusion protein of porcine IFN- λ 1 and GP5 gene of porcine reproductive and respiratory syndrome virus.
Porcine reproductive and respiratory syndrome virus (PRRSV) has been mainly responsible for the catastrophic economic losses in pig industry worldwide. The commercial vaccines only provide a limited protection against PRRSV infection. Thus, the focus and direction is to develop safer and more effective vaccines in the research field of PRRS. The immune modulators are being considered to enhance the effectiveness of PRRSV vaccines. IFN- λ1 belongs to type III interferon, a new interferon family. IFN- λ1 is an important cytokine with multiple functions in innate and acquired immunity. In this study, porcine IFN- λ1 (PoIFN- λ1) was evaluated for its adjuvant effects on the immunity of a DNA vaccine carrying the GP5 gene of PRRSV. Groups of mice were immunized twice at 2-week interval with 100 μ g of the plasmid DNA vaccine pcDNA3.1-SynORF5, pcDNA3.1-PoIFN- λ1-SynORF5, and the blank vector pcDNA3.1, respectively. The results showed that pcDNA3.1-PoIFN- λ1-SynORF5 can significantly enhance GP5-specific ELISA antibody, PRRSV-specific neutralizing antibody, IFN- γ level, and lymphocyte proliferation rather than the responses induced by pcDNA3.1-SynORF5. Therefore, type III interferon PoIFN- λ1 could enhance the immune responses of DNA vaccine of PRRSV, highlighting the potential value of PoIFN- λ1 as a molecular adjuvant in the prevention of PRRSV infection.
College of Veterinary Medicine, Nanjing Agricultural University, No. 1 Wei-gang, Nanjing 210095, China ; Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu Province 210014, China ; Key Laboratory of Animal Diseases, Diagnostics, and Immunology, Ministry of Agriculture, Nanjing, Jiangsu Province 210014, China ; National Center for Engineering Research of Veterinary Bio-Products, Nanjing, Jiangsu Province 210014, China.
Luping
L
Du
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu Province 210014, China ; Key Laboratory of Animal Diseases, Diagnostics, and Immunology, Ministry of Agriculture, Nanjing, Jiangsu Province 210014, China ; National Center for Engineering Research of Veterinary Bio-Products, Nanjing, Jiangsu Province 210014, China ; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu Province 225009, China.
Bin
B
Li
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu Province 210014, China ; Key Laboratory of Animal Diseases, Diagnostics, and Immunology, Ministry of Agriculture, Nanjing, Jiangsu Province 210014, China ; National Center for Engineering Research of Veterinary Bio-Products, Nanjing, Jiangsu Province 210014, China ; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu Province 225009, China.
Kongwang
K
He
College of Veterinary Medicine, Nanjing Agricultural University, No. 1 Wei-gang, Nanjing 210095, China.
Haibin
H
Zhang
College of Veterinary Medicine, Nanjing Agricultural University, No. 1 Wei-gang, Nanjing 210095, China.
Kehe
K
Huang
State Key Laboratory of Agricultural Microbiology, Division of Animal Infectious Diseases, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Shaobo
S
Xiao
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D007371:Interferon-gamma / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D051379:Mice; D019318:Porcine Reproductive and Respiratory Syndrome / Q000188:drug therapy* / Q000276:immunology / Q000517:prevention & control; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D013552:Swine; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D011993:Recombinant Fusion Proteins; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; C114789:glycoprotein 5, PRRSV; D007371:Interferon-gamma