The grapevine flagellin receptor VvFLS2 differentially recognizes flagellin-derived epitopes from the endophytic growth-promoting bacterium Burkholderia phytofirmans and plant pathogenic bacteria.
• The role of flagellin perception in the context of plant beneficial bacteria still remains unclear. Here, we characterized the flagellin sensing system flg22-FLAGELLIN SENSING 2 (FLS2) in grapevine, and analyzed the flagellin perception in the interaction with the endophytic plant growth-promoting rhizobacterium (PGPR) Burkholderia phytofirmans. • The functionality of the grapevine FLS2 receptor, VvFLS2, was demonstrated by complementation assays in the Arabidopsis thaliana fls2 mutant, which restored flg22-induced H₂O₂ production and growth inhibition. Using synthetic flg22 peptides from different bacterial origins, we compared recognition specificities between VvFLS2 and AtFLS2. • In grapevine, flg22-triggered immune responses are conserved and led to partial resistance against Botrytis cinerea. Unlike flg22 peptides derived from Pseudomonas aeruginosa or Xanthomonas campestris, flg22 peptide derived from B. phytofirmans triggered only a small oxidative burst, weak and transient defense gene induction and no growth inhibition in grapevine. Although, in Arabidopsis, all the flg22 epitopes exhibited similar biological activities, the expression of VvFLS2 into the fls2 background conferred differential flg22 responses characteristic for grapevine. • These results demonstrate that VvFLS2 differentially recognizes flg22 from different bacteria, and suggest that flagellin from the beneficial PGPR B. phytofirmans has evolved to evade this grapevine immune recognition system.
Université de Bourgogne, UMR 1347 Agroécologie, Pôle Interactions Plantes Micro-organismes - ERL CNRS 6300, 17 rue Sully, 21000, Dijon, France.
Lucie
L
Trdá
Laboratoire Stress, Défenses et Reproduction des Plantes, URVVC EA 4707, Université de Reims Champagne-Ardenne, Campus Moulin de la Housse Chemin des Rouliers, 51687, Reims, France.
Olivier
O
Fernandez
The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK.
Freddy
F
Boutrot
Université de Bourgogne, UMR 1347 Agroécologie, Pôle Interactions Plantes Micro-organismes - ERL CNRS 6300, 17 rue Sully, 21000, Dijon, France.
Marie-Claire
MC
Héloir
Université de Bourgogne, UMR 1347 Agroécologie, Pôle Interactions Plantes Micro-organismes - ERL CNRS 6300, 17 rue Sully, 21000, Dijon, France.
Jani
J
Kelloniemi
INRA, UMR 1347 Agroécologie, Pôle Interactions Plantes Micro-organismes - ERL CNRS 6300, 17 rue Sully, 21000, Dijon, France.
Xavier
X
Daire
Université de Bourgogne, UMR 1347 Agroécologie, Pôle Interactions Plantes Micro-organismes - ERL CNRS 6300, 17 rue Sully, 21000, Dijon, France.
Marielle
M
Adrian
Laboratoire Stress, Défenses et Reproduction des Plantes, URVVC EA 4707, Université de Reims Champagne-Ardenne, Campus Moulin de la Housse Chemin des Rouliers, 51687, Reims, France.
Christophe
C
Clément
The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK.
Cyril
C
Zipfel
Laboratoire Stress, Défenses et Reproduction des Plantes, URVVC EA 4707, Université de Reims Champagne-Ardenne, Campus Moulin de la Housse Chemin des Rouliers, 51687, Reims, France.
Stéphan
S
Dorey
Université de Bourgogne, UMR 1347 Agroécologie, Pôle Interactions Plantes Micro-organismes - ERL CNRS 6300, 17 rue Sully, 21000, Dijon, France.
Benoit
B
Poinssot
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000502:physiology; D020171:Botrytis / Q000187:drug effects / Q000502:physiology; D019117:Burkholderia / Q000254:growth & development / Q000502:physiology*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D015169:Colony Count, Microbial; D003198:Computer Simulation; D060467:Disease Resistance / Q000187:drug effects; D060026:Endophytes / Q000187:drug effects / Q000254:growth & development*; D000939:Epitopes / Q000276:immunology*; D005408:Flagellin / Q000276:immunology* / Q000494:pharmacology; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology; D057865:Plant Immunity / Q000187:drug effects; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000378:metabolism*; D013045:Species Specificity; D027843:Vitis / Q000187:drug effects / Q000254:growth & development / Q000276:immunology* / Q000382:microbiology*
D000939:Epitopes; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D011956:Receptors, Cell Surface; D005408:Flagellin
Burkholderia phytofirmans; PGPR; Vitis vinifera; flagellin sensing; flg22; microbe-associated molecular pattern (MAMP); pattern recognition receptor (PRR)