[Regulation effects of intracellular and extracellular Ca2+ on biosynthesis of rosmarinic acid induced by salicylic acid in young seedlings of Salvia miltiorrhiza].
OBJECTIVE
To investigate the effect of intracellular and extracellular Ca2+ on the biosynthesis of rosmarinic acid (RA) induced by salicylic acid in young seedlings of Salvia miltiorrhiza.


METHODS
Young seedlings of S. miltiorrhiza were used to select an optimal concentration of salicylic acid (SA), and then use the optimal concentration of SA to investigate the effects of extracellular Ca2+ channel inhibitors Verapamil, LaCl3, intracelluar calmodulin antagonist TFP and intracelluar Ca2+ channel inhibitors LiCl on the biosynthesis of RA and related enzymes.


RESULTS
SA increased the accumulation of RA and the activities of PAL and TAT, especially the SA of 2 mmol x L(-1) after 24 h. SA improved the accumulation of RA to (40.51 +/- 2.16) mg x g(-1), which was 1.97 times than that of control, and the activities of PAL, TAT were 1.42 times and 1.29 times than those of the control. However, Vp, LaCl3, TFP, LiCl inhibited the effects of SA evidently.


CONCLUSIONS
Ca2+ plays a key role in the regulation of the induction process.
College of Life Sciences, Northwest Agricultural and Forestry University, Yangling 712100, China.
Rong-Rong
RR
Cao
College of Life Sciences, Northwest Agricultural and Forestry University, Yangling 712100, China.
Xiao-Lin
XL
Dang
College of Life Sciences, Northwest Agricultural and Forestry University, Yangling 712100, China.
Bing-Yu
BY
Xing
College of Life Sciences, Northwest Agricultural and Forestry University, Yangling 712100, China.
Jing-Yi
JY
Zhang
College of Life Sciences, Northwest Agricultural and Forestry University, Yangling 712100, China.
Juan-E
JE
Dong
D002118:Calcium / Q000378:metabolism*; D002934:Cinnamates / Q000378:metabolism*; D053630:Depsides / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D020156:Salicylic Acid / Q000378:metabolism*; D027561:Salvia miltiorrhiza / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D036226:Seedlings / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D000097123:Rosmarinic Acid
D002118:Calcium; D002934:Cinnamates; D053630:Depsides; D010940:Plant Proteins; D020156:Salicylic Acid; D000097123:Rosmarinic Acid