A CMOS cantilever-based label-free DNA SoC with improved sensitivity for hepatitis B virus detection.
This paper presents a highly-integrated DNA detection SoC, where several kinds of cantilever DNA sensors, a readout circuit, an MCU, voltage regulators, and a wireless transceiver, are integrated monolithically in a 0.35 μm CMOS Bio-MEMS process. The cantilever-based biosensors with embedded piezoresistors aim to transduce DNA hybridization into resistance variation without cumbersome labeling process. To improve detection sensitivity for low DNA concentration use, an oscillator-based self-calibrated readout circuit with high precision is proposed to convert small resistance variation ( of original resistance) of the sensor into adequate frequency variation and further into digital data. Moreover, its wireless capacity enables isolation of the sample solution from electrical wire lines and facilitates data transmission. To demonstrate the effectiveness of full system, it is applied to detect hepatitis B virus (HBV) DNA. The experimental results show that it has the capability to distinguish between one base-pair (1-bp) mismatch DNAs and match DNAs and achieves a limit of detection (LOD) of less than 1 pM.
Yu-Jie
YJ
Huang
Che-Wei
CW
Huang
Tsung-Hsien
TH
Lin
Chih-Ting
CT
Lin
Li-Guang
LG
Chen
Po-Yun
PY
Hsiao
Bi-Ru
BR
Wu
Hsiao-Ting
HT
Hsueh
Bing-Jye
BJ
Kuo
Hann-Huei
HH
Tsai
Hsin-Hao
HH
Liao
Ying-Zong
YZ
Juang
Chorng-Kuang
CK
Wang
Shey-Shi
SS
Lu
D015374:Biosensing Techniques / Q000295:instrumentation*; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification*; D056656:Lab-On-A-Chip Devices; D057230:Limit of Detection; D015336:Molecular Probe Techniques / Q000295:instrumentation; D012666:Semiconductors
D004279:DNA, Viral