Combination of micropreparative solution isoelectric focusing and high-performance liquid chromatography for differentiation of biofilm-positive and biofilm-negative Candida parapsilosis group from vascular catheter.
This study utilizes the high-performance liquid chromatography technique in combination with the new micropreparative solution isoelectric focusing fractionation on non-woven fabric strip for the characterization and differentiation of biofilm-positive and biofilm-negative forms of Candida parapsilosis sensu stricto on the basis of the changes in the composition of their cell-surface. Treatment of yeasts by boiling in distilled water relased surface substances from yeasts cells. Consequently, the optimized procedure has been used for fast identification of the highly pathogenic biofilm-positive Candida parapsilosis group in real clinical material - sonicate from vascular catheters. Moreover, the capillary isoelectric focusing was used as supporting and control technique. Obtained results suggest that this new method can be used to distinguish between biofilm-positive and negative forms of Candida parapsilosis sensu stricto.
Institute of Analytical Chemistry of the ASCR, v. v. i., Veveří 97, 602 00 Brno, Czech Republic; Department of Biochemistry, Faculty of Science, Masaryk University, Kotlářská 267/2, 602 00 37 Brno, Czech Republic. Electronic address: vykydalova@iach.cz.
Marie
M
Vykydalová
Institute of Analytical Chemistry of the ASCR, v. v. i., Veveří 97, 602 00 Brno, Czech Republic.
Marie
M
Horká
Department of Microbiology, Faculty of Medicine, Masaryk University, Pekařská 53, 602 00 Brno, Czech Republic. Electronic address: filip.ruzicka@fnusa.cz.
Filip
F
Růžička
Institute of Analytical Chemistry of the ASCR, v. v. i., Veveří 97, 602 00 Brno, Czech Republic; Department of Biochemistry, Faculty of Science, Masaryk University, Kotlářská 267/2, 602 00 37 Brno, Czech Republic.
Filip
F
Duša
Institute of Analytical Chemistry of the ASCR, v. v. i., Veveří 97, 602 00 Brno, Czech Republic.
Dana
D
Moravcová
Institute of Analytical Chemistry of the ASCR, v. v. i., Veveří 97, 602 00 Brno, Czech Republic.
Vladislav
V
Kahle
Institute of Analytical Chemistry of the ASCR, v. v. i., Veveří 97, 602 00 Brno, Czech Republic.
Karel
K
Slais
D018441:Biofilms; D002175:Candida / Q000302:isolation & purification*; D002408:Catheters, Indwelling / Q000382:microbiology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing / Q000379:methods*
Biofilm; Candida parapsilosis group; Capillary isoelectric focusing; High-performance liquid chromatography; Micropreparative solution isoelectric focusing; Sonicate from vascular catheter