Influence on functional parameters of intestinal tract induced by short-term exposure to fumonisins contaminated corn chyme samples.
The gut is a possible target toward mycotoxin fumonisins (FBs) exposure. The study aims to investigate the effects induced by FBs contaminated-corn chyme samples on functional parameters of human and rat intestine by using Ussing chamber. Fumonisins-contaminated corn and processed corn samples were undergone to in vitro digestion process and then added to luminal side. A reduction (about 90%) of short circuit current (Isc μA/cm(2)) during exposure of human colon tissues to fumonisins-free corn chyme samples was observed, probably related to increased chyme osmolality. This hyperosmotic stress could drain water towards the luminal compartment, modifying Na(+) and Cl(-) transports. The presence of FBs in corn chyme samples, independently to their concentration, did not affect significantly the Isc, probably related to their interference towards epithelial Na(+) transport, as assessed by using a specific inhibitor (Amiloride). The rat colon tract represents a more accessible model to study FBs toxicity showing a similar functional response to human. In the rat small intestine a significant reduction (about 15%) of Isc parameter during exposure to uncontaminated or FBs contaminated corn chyme samples was observed; therefore such model was not suitable to assess the FBs toxicity, probably because the prevalent glucose and amino acids electrogenic absorption overwhelmed the FBs influence on ionic transport.
National Research Council of Italy (CNR), Institute of Sciences of Food Production (ISPA), Via G. Amendola 122/O, 70125 Bari, Italy. Electronic address: Fiorenza.minervini@ispa.cnr.it.
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Minervini
Department of Bioscience, Biotechnologies and Biopharmaceutics, University of Bari "Aldo Moro", Via E. Orabona 4, 70125 Bari, Italy. Electronic address: lucantonio.debellis@uniba.it.
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Debellis
National Research Council of Italy (CNR), Institute of Sciences of Food Production (ISPA), Via G. Amendola 122/O, 70125 Bari, Italy. Electronic address: Antonella.garbetta@ispa.cnr.it.
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Garbetta
National Research Council of Italy (CNR), Institute of Sciences of Food Production (ISPA), Via G. Amendola 122/O, 70125 Bari, Italy. Electronic address: Annalisa.degirolamo@ispa.cnr.it.
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De Girolamo
National Research Council of Italy (CNR), Institute of Sciences of Food Production (ISPA), Via G. Amendola 122/O, 70125 Bari, Italy. Electronic address: Roberto.schena@ispa.cnr.it.
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Schena
Department of Biomedical Sciences & Human Oncology, University of Bari Medical School, Piazza G. Cesare, 70100 Bari, Italy. Electronic address: piero.portincasa@uniba.it.
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Portincasa
National Research Council of Italy (CNR), Institute of Sciences of Food Production (ISPA), Via G. Amendola 122/O, 70125 Bari, Italy. Electronic address: Angelo.visconti@ispa.cnr.it.
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Visconti
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D005260:Female; D037341:Fumonisins / Q000633:toxicity*; D006801:Humans; D007421:Intestine, Small / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D051381:Rats; D003313:Zea mays / Q000737:chemistry*
D037341:Fumonisins
Electrophysiological parameter; Fumonisin; Human and rat intestine tract; In vitro digestion; Ussing chamber