Peroxiredoxin 1 is involved in disassembly of flagella and cilia.
Cilia/flagella are evolutionarily conserved cellular organelles. In this study, we demonstrated that Dunaliella salina Peroxiredoxin 1 (DsPrdx1) localized to the flagella and basal bodies, and was involved in flagellar disassembly. The link between DsPrdx1 and flagella of Dunaliella salina (D. salina) encouraged us to explore the function of its human homologue, Homo sapiens Peroxiredoxin 1 (HsPrdx1) in development and physiology. Our results showed that HsPrdx1 was overexpressed, and cilia were lost in esophageal squamous cell carcinoma (ESCC) cells compared with the non-cancerous esophageal epithelial cells Het-1A. Furthermore, when HsPrdx1 was knocked down by short hairpin RNA (shRNA) lentivirus in ESCC cells, the phenotype of cilia lost can be reversed, and the expression levels of tumor suppressor genes LKB1 and p-AMPK were increased, and the activity of the oncogene Aurora A was inhibited compared with those in cells transfected with scrambe-shRNA lentivirus. These findings firstly showed that Prdx1 is involved in disassembly of flagella and cilia, and suggested that the abnormal expression of the cilia-related gene including Prdx1 may affect both ciliogenesis and cancernogenesis.
Laboratory for Cell Biology, The First Affiliated Hospital, Zhengzhou University, 40 Daxue Road, Zhengzhou 450052, China.
Fanghua
F
Gong
College of Life Sciences, Zhengzhou University, 100 Kexue Avenue, Zhengzhou 450001, China.
Hongtao
H
Liu
College of Life Sciences, Zhengzhou University, 100 Kexue Avenue, Zhengzhou 450001, China. Electronic address: lijie20141101@163.com.
Jie
J
Li
Laboratory for Cell Biology, The First Affiliated Hospital, Zhengzhou University, 40 Daxue Road, Zhengzhou 450052, China.
Lexun
L
Xue
Department of Oncology, The First Affiliated Hospital, Zhengzhou University, 1 East Jianshe Road, Zhengzhou 450052, China. Electronic address: zhangmzzdyxy@163.com.
Mingzhi
M
Zhang
D000595:Amino Acid Sequence; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D000460:Chlorophyta / Q000502:physiology; D004938:Esophageal Neoplasms / Q000378:metabolism / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D005407:Flagella / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D054464:Peroxiredoxins / Q000502:physiology*; D017386:Sequence Homology, Amino Acid
C515940:PRDX1 protein, human; D054464:Peroxiredoxins
Dunaliella salina; Esophageal squamous cell carcinoma; Flagellar/ciliary disassembly; Peroxiredoxin 1