[Standardization of cryopreservation process of Saccharomyces boulardii yeasts for usage in collections and banks of industrial microorganism strains].
New implementation principle of freeze-thawing during cryopreservation of microorganisms, initiation of the process of crystal formation at cooling and thawing stage with the controlled rate of heating was experimentally substantiated. This allows increasing the cell viability, guaranteeing their equal number in each preparation, decreasing the contamination risk of the samples at thawing stage.
T M
TM
Gurina
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IP
Vysekantsev
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OM
Babinets
D001515:Beer; D018070:Biological Specimen Banks; D015169:Colony Count, Microbial; D015925:Cryopreservation / Q000379:methods* / Q000592:standards; D003451:Cryoprotective Agents / Q000494:pharmacology*; D003460:Crystallization; D007218:Industrial Microbiology; D050296:Microbial Viability; D012440:Saccharomyces / Q000187:drug effects* / Q000502:physiology
D003451:Cryoprotective Agents