Inhibition of human neutrophils NEP activity, CD11b/CD18 expression and elastase release by 3,4-dihydroxyphenylethanol-elenolic acid dialdehyde, oleacein.
Polyphenols, such as oleacein (3,4-DHPEA-EDA; 3,4-dihydroxyphenylethanol-elenolic acid dialdehyde), are believed to play a role in the prevention of cardiovascular diseases. Due to an increase of neutrophil mediators in acute myocardial infarction the aim of this study was to establish the effect of oleacein on neutral endopeptidase (NEP) activity and other functions of human neutrophils, such as elastase, MMP-9 and IL-8 production. The effect on CD62L and CD11b/CD18 expression on neutrophils was also determined. Oleacein with a concentration of 100 μM inhibited NEP activity, elastase, MMP-9 and IL-8 release from neutrophils by 77.7 ± 2.7%, 21.3 ± 7.8%, 22.7 ± 4.2% and 25.2 ± 5.6%, respectively. Oleacein with a concentration of 50 μM suppressed CD11b/CD18 expression by 63.6 ± 3.1% and to a lesser extent, increased CD62L expression by 27.3 ± 8.3% on the surface of neutrophils, in comparison with stimulated cells. Oleacein by inhibiting NEP activity, adhesion molecules expression and elastase release might play a role in the protective effects of olive oil against endothelial injuries.
Department of Pharmacognosy and Molecular Basis of Phytotherapy, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland.
Monika E
ME
Czerwińska
Department of Pharmacognosy and Molecular Basis of Phytotherapy, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland.
Anna K
AK
Kiss
Department of Pharmacognosy and Molecular Basis of Phytotherapy, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland. Electronic address: marek.naruszewicz@wum.edu.pl.
Marek
M
Naruszewicz
D000447:Aldehydes / Q000494:pharmacology*; D039481:CD11b Antigen / Q000235:genetics* / Q000378:metabolism; D018821:CD18 Antigens / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D015260:Neprilysin / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D000069463:Olive Oil; D010196:Pancreatic Elastase / Q000235:genetics / Q000378:metabolism*; D010636:Phenols / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D010938:Plant Oils / Q000737:chemistry; D011714:Pyrans / Q000494:pharmacology*
C547883:3,4-dihydroxyphenylethanol-elenolic acid dialdehyde; D000447:Aldehydes; D039481:CD11b Antigen; D018821:CD18 Antigens; D004791:Enzyme Inhibitors; C509295:ITGAM protein, human; D000069463:Olive Oil; D010636:Phenols; D010936:Plant Extracts; D010938:Plant Oils; D011714:Pyrans; C578055:oleacein; D010196:Pancreatic Elastase; D015260:Neprilysin
Cardiovascular diseases; DHPEA-EDA; Human neutrophils; Oleacein; Olive oil