A PCR-high-resolution melt assay for rapid differentiation of nontypeable Haemophilus influenzae and Haemophilus haemolyticus.
We have developed a PCR-high-resolution melt (PCR-HRM) assay to discriminate nontypeable Haemophilus influenzae (NTHi) colonies from Haemophilus haemolyticus. This method is rapid and robust, with 96% sensitivity and 92% specificity compared to the hpd#3 assay. PCR-HRM is ideal for high-throughput screening for NTHi surveillance and clinical trials.
School of Paediatrics and Child Health, The University of Western Australia, Perth, Australia.
Janessa
J
Pickering
Michael J
MJ
Binks
Jemima
J
Beissbarth
Kim M
KM
Hare
Lea-Ann S
LA
Kirkham
Heidi
H
Smith-Vaughan
D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006190:Haemophilus / Q000145:classification* / Q000235:genetics*; D006192:Haemophilus Infections / Q000175:diagnosis* / Q000382:microbiology*; D057166:High-Throughput Screening Assays; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D044366:Transition Temperature
D004269:DNA, Bacterial