De novo genome assembly of the fungal plant pathogen Pyrenophora semeniperda.
Pyrenophora semeniperda (anamorph Drechslera campulata) is a necrotrophic fungal seed pathogen that has a wide host range within the Poaceae. One of its hosts is cheatgrass (Bromus tectorum), a species exotic to the United States that has invaded natural ecosystems of the Intermountain West. As a natural pathogen of cheatgrass, P. semeniperda has potential as a biocontrol agent due to its effectiveness at killing seeds within the seed bank; however, few genetic resources exist for the fungus. Here, the genome of P. semeniperda isolate assembled from sequence reads of 454 pyrosequencing is presented. The total assembly is 32.5 Mb and includes 11,453 gene models encoding putative proteins larger than 24 amino acids. The models represent a variety of putative genes that are involved in pathogenic pathways typically found in necrotrophic fungi. In addition, extensive rearrangements, including inter- and intrachromosomal rearrangements, were found when the P. semeniperda genome was compared to P. tritici-repentis, a related fungal species.
Plant and Wildlife Sciences, Brigham Young University, Provo, Utah, United States of America.
Marcus M
MM
Soliai
USDA Forest Service, Rocky Mountain Research Station, Shrub Sciences Laboratory, Provo, Utah, United States of America.
Susan E
SE
Meyer
Plant and Wildlife Sciences, Brigham Young University, Provo, Utah, United States of America.
Joshua A
JA
Udall
Plant and Wildlife Sciences, Brigham Young University, Provo, Utah, United States of America.
David E
DE
Elzinga
Plant and Wildlife Sciences, Brigham Young University, Provo, Utah, United States of America.
Russell A
RA
Hermansen
Plant and Wildlife Sciences, Brigham Young University, Provo, Utah, United States of America.
Paul M
PM
Bodily
Plant and Wildlife Sciences, Brigham Young University, Provo, Utah, United States of America.
Aaron A
AA
Hart
Plant and Wildlife Sciences, Brigham Young University, Provo, Utah, United States of America.
Craig E
CE
Coleman
D001203:Ascomycota / Q000235:genetics*; D001483:Base Sequence; D031724:Bromus / Q000382:microbiology*; D018076:DNA, Complementary / Q000235:genetics; D040481:Genome Components / Q000235:genetics*; D016681:Genome, Fungal / Q000235:genetics*; D007061:Idaho; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000235:genetics; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D009841:Oligonucleotides