Reconstitution of coronary vasculature by an active fraction of Geum japonicum in ischemic hearts.
Chronic coronary heart disease (cCHD) is characterized by atherosclerosis, which progressively narrows the coronary artery lumen and impairs myocardial blood flow. Restoration of occluded coronary vessels with newly formed collaterals remains an ideal therapeutic approach due to the need for redirecting blood flow into the ischemic heart. In this study, we investigated the effect of an active fraction isolated from Geum joponicum (AFGJ) on angiogenesis in cCHD hearts. Our results demonstrated that AFGJ not only enhanced capillary tube formation of endothelial cells, but also promoted the growth of new coronary collaterals (at the diameter 0.021-0.21 mm) in the ischemic region of hearts in rat cCHD model. Our study also indicated that the growth of new collaterals in ischemic hearts resulted in improved functional recovery of the cCHD hearts as demonstrated by ECG and echocardiography analyses. These data suggest that AFGJ may provide a novel therapeutic method for effective treatment of cCHD.
1] The Second People's Hospital of Shenzhen, The First Affiliated Hospital of Shenzhen University, Shenzhen, PR China [2] Joint Laboratory For Regenerative Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong.
Hao
H
Chen
Joint Laboratory For Regenerative Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong.
Lei
L
Cheng
Laboratory of Innovative Medicine, Hong Kong.
Xiaoli
X
Lin
The Second People's Hospital of Shenzhen, The First Affiliated Hospital of Shenzhen University, Shenzhen, PR China.
Xiaping
X
Zhou
The Second People's Hospital of Shenzhen, The First Affiliated Hospital of Shenzhen University, Shenzhen, PR China.
Zhiming
Z
Cai
Laboratory of Innovative Medicine, Hong Kong.
Ming
M
Li
D000818:Animals; D050197:Atherosclerosis / Q000188:drug therapy; D049109:Cell Proliferation; D002478:Cells, Cultured; D003327:Coronary Disease / Q000188:drug therapy*; D003331:Coronary Vessels / Q000187:drug effects; D004452:Echocardiography; D031986:Geum / Q000378:metabolism*; D006321:Heart; D008297:Male; D009203:Myocardial Infarction / Q000188:drug therapy*; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D010936:Plant Extracts