⁹⁹mTc carbonyl DTPA-rituximab: preparation and preliminary bioevaluation.
The anti CD20 antibody Rituximab was conjugated with para isothiocyanato benzyl diethylene triamine penta acetic acid (p-NCS-Bz-DTPA) and subsequent radiolabeling with (99m)Tc was carried out via the (99m)Tc carbonyl synthon. The (99m)Tc labeled antibody conjugate exhibited >95% radiochemical purity after purification and retained good in vitro stability when studied up to 24h at room temperature. In vitro cell binding studies carried out in Raji cells expressing CD20 antigen validated the biological efficacy of the preparation.
Radiopharmaceuticals Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India.
Usha
U
Pandey
Radiopharmaceuticals Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India.
Mythili
M
Kameswaran
Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
Haladhar
H
Dev Sarma
Radiopharmaceuticals Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India. Electronic address: grace@barc.gov.in.
Grace
G
Samuel
D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000737:chemistry; D018951:Antigens, CD20 / Q000378:metabolism; D051379:Mice; D015609:Organotechnetium Compounds / Q000138:chemical synthesis*; D019275:Radiopharmaceuticals / Q000138:chemical synthesis*; D000069283:Rituximab; D014018:Tissue Distribution
D058846:Antibodies, Monoclonal, Murine-Derived; D018951:Antigens, CD20; D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals; C000592761:technetium 99m carbonyl DTPA-rituximab; D000069283:Rituximab
(99m)Tc carbonyl–DTPA–Rituximab; Non-Hodgkin's lymphoma (NHL); Radioimmunoscintigraphy; Rituximab