Transcription profiling of 12 asian gypsy moth (Lymantria dispar) cytochrome P450 genes in response to insecticides.
As the main group of detoxification enzymes, cytochrome P450 monoxygenases (P450s) catalyse an extremely diverse range of reactions that play an important role in the detoxification of foreign compounds. Transcription profiling of 12 Lymantria dispar P450 genes from the CYP6 subfamily believed to be involved in insecticide metabolism was performed in this study. Life-stage transcription profiling of CYP6 genes revealed significant variations between eggs, larvae, pupae, and adult males and females. Exposure of larvae to sublethal doses of deltamethrin, omethoate, and carbaryl enhanced the transcription of most of the CYP6 P450 genes, with induction peaking between 24 and 72 h after exposure. Transcription profiles were dependent on the levels of insecticide exposure and the various developmental stages.
School of Forestry, Northeast Forestry University, Harbin, P. R. China.
Lili
L
Sun
Zhiying
Z
Wang
Chuanshan
C
Zou
Chuanwang
C
Cao
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D008658:Inactivation, Metabolic; D007306:Insecticides / Q000378:metabolism* / Q000633:toxicity; D008018:Life Cycle Stages; D008297:Male; D009036:Moths / Q000201:enzymology / Q000235:genetics*; D009570:Nitriles / Q000633:toxicity; D010802:Phylogeny; D011722:Pyrethrins / Q000633:toxicity; D012737:Sex Factors
D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin; D003577:Cytochrome P-450 Enzyme System
Asian gypsy moth; CYP6 subfamily; P450; insecticide; transcription profiling