Asymmetric spindle pole formation in CPAP-depleted mitotic cells.
CPAP is an essential component for centriole formation. Here, we report that CPAP is also critical for symmetric spindle pole formation during mitosis. We observed that pericentriolar material between the mitotic spindle poles were asymmetrically distributed in CPAP-depleted cells even with intact numbers of centrioles. The length of procentrioles was slightly reduced by CPAP depletion, but the length of mother centrioles was not affected. Surprisingly, the young mother centrioles of the CPAP-depleted cells are not fully matured, as evidenced by the absence of distal and subdistal appendage proteins. We propose that the selective absence of centriolar appendages at the young mother centrioles may be responsible for asymmetric spindle pole formation in CPAP-depleted cells. Our results suggest that the neural stem cells with CPAP mutations might form asymmetric spindle poles, which results in premature initiation of differentiation.
Department of Biological Sciences, Seoul National University, Seoul, Republic of Korea.
Miseon
M
Lee
Department of Biological Sciences, Seoul National University, Seoul, Republic of Korea.
Jaerak
J
Chang
Department of Physiology and Biomedical Sciences, Seoul National University, Seoul, Republic of Korea.
Sunghoe
S
Chang
National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Kyung S
KS
Lee
Department of Biological Sciences, Seoul National University, Seoul, Republic of Korea. Electronic address: rheek@snu.ac.kr.
Kunsoo
K
Rhee
D002502:Centrioles / Q000235:genetics / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D008938:Mitosis; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D064047:Spindle Poles / Q000235:genetics* / Q000648:ultrastructure
C414735:CENPJ protein, human; D008869:Microtubule-Associated Proteins; D034741:RNA, Small Interfering
Asymmetric cell division; CPAP; Centriole maturation; Spindle pole