Nonradioactive in situ hybridization on skeletal tissue sections.
Spatial and temporal visualization of RNA transcripts in tissue is a key tool in studying both developmental and pathological processes. In situ hybridization is a highly sensitive method for RNA transcript detection. It is based on sequence complementation between a labeled RNA probe and the RNA transcript of interest. The labeled probe is then detected by immunohistochemical methods using an antibody conjugated to an enzyme that catalyzes the generation of chromogenic or fluorescent signals, which indicate the location of the transcript.
Weizmann Institute of Science, Rehovot, Israel.
Yulia
Y
Shwartz
Weizmann Institute of Science, Rehovot, Israel.
Elazar
E
Zelzer
D000818:Animals; D001842:Bone and Bones / Q000378:metabolism*; D005456:Fluorescent Dyes; D017403:In Situ Hybridization / Q000379:methods*; D051379:Mice; D008856:Microscopy, Fluorescence; D015347:RNA Probes
D005456:Fluorescent Dyes; D015347:RNA Probes