Mixed nanomicelles loaded with thymopeptides-sodium deoxycholate/phospholipid improve drug absorption.
OBJECTIVE
To improve the absorption of thymopeptides (TH) by preparing sodium deoxycholate/phospholipid-mixed nanomicelles (SDC/PL-MMs).


METHODS
TH-SDC/PL-MMs were prepared by a film dispersion method, and then evaluated using photon correlation spectroscopy (PCS), zeta potential measurement, as well as their physical stability after storage for several days. Furthermore, in situ intestinal single-pass perfusion experiments and pharmacodynamics in immunodeficient mice were performed to make a comparison with TH powders and the control drug in absorption properties.


RESULTS
A narrow size distribution of nanomicelles, with a mean particle size of (149 ± 8.32) nm and a zeta potential of (-31.05 ± 2.52) mV, was obtained. The in situ intestine perfusion experiments demonstrated a significant advantage in absorption characteristics for TH compared to the other formulations, and oral administration of TH-SDC/PL-MMs potentiated an equivalent effect with i.h. TH in pharmacodynamic studies in immunodeficient mice.


CONCLUSIONS
TH-SDC/PL-MMs prepared by a film dispersion method are able to improve the absorption of TH. SDC/PL-MMs might be a good approach for the more effective delivery of drugs like TH.
Department of Pharmacy, 302 Military Hospital, Beijing 100039, China; School of pharmaceutics, Chengdu University of TCM, Chengdu 611730, China.
Jin
J
Shi-Xiao
Department of Pharmacy, 302 Military Hospital, Beijing 100039, China; School of pharmaceutics, Chengdu University of TCM, Chengdu 611730, China.
Jin
J
Shi-Ying
Department of Pharmacy, 302 Military Hospital, Beijing 100039, China.
Qing-Yuan
QY
Lv
Department of Pharmacy, 302 Military Hospital, Beijing 100039, China; School of pharmaceutics, Chengdu University of TCM, Chengdu 611730, China.
Fu
F
Shan-Shan
Department of Pharmacy, 302 Military Hospital, Beijing 100039, China. Electronic address: hanjin302emba@163.com.
Han
H
Jin
Department of Pharmacy, 302 Military Hospital, Beijing 100039, China. Electronic address: yhlpharm@126.com.
Hai-Long
HL
Yuan
D000818:Animals; D002626:Chemistry, Pharmaceutical; D003840:Deoxycholic Acid / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D004355:Drug Stability; D051379:Mice; D008823:Micelles; D010316:Particle Size; D010455:Peptides / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics*; D010743:Phospholipids / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013950:Thymus Gland / Q000737:chemistry*
D004337:Drug Carriers; D008823:Micelles; D010455:Peptides; D010743:Phospholipids; D003840:Deoxycholic Acid
Immunodeficiency; In situ intestinal absorption; Micelles; Phospholipid; R283.6; Sodium deoxycholate; Thymopeptides