Antrodia salmonea in submerged culture exhibits antioxidant activities in vitro and protects human erythrocytes and low-density lipoproteins from oxidative modification.
Antrodia salmonea is well known in Taiwan as a beneficial mushroom. In the present study, we investigated the antioxidant activity of whole fermented broth (AS), filtrate (ASF), and mycelia (ASM) of A. salmonea using different antioxidant models. Furthermore, the effect of A. salmonea on AAPH-induced oxidative hemolysis of human erythrocytes and CuSO4-induced oxidative modification of human low-density lipoproteins (LDLs) was examined. We found that the AS, ASF, and ASM possess effective antioxidant activity against various oxidative systems including superoxide anion scavenging, reducing power, metal chelation, and DPPH radical scavenging. Further, AAPH-induced oxidative hemolysis in erythrocytes was prevented by AS, ASF, and ASM. Notably, AS, ASF, and ASM appear to possess powerful antioxidant activities against CuSO4-induced oxidative modification of LDL as assessed by malondialdehyde (MDA) formation, cholesterol degradation, and the relative electrophoretic mobility of oxidized LDL. It is noteworthy that AS had comparatively strong antioxidant ability compared to ASF or ASM, which is well correlated with the content of their total polyphenols. Thus, A. salmonea may exert antioxidant properties and offer protection from atherogenesis.
Department of Cosmeceutics, China Medical University, Taichung 40402, Taiwan; Department of Health and Nutrition Biotechnology, Asia University, Taichung 41354, Taiwan.
You-Cheng
YC
Hseu
Department of Health and Nutrition Biotechnology, Asia University, Taichung 41354, Taiwan.
Chuan-Chen
CC
Lee
Department of Applied Chemistry, Chao Yang University of Technology, Taichung 41349, Taiwan.
Yung-Chang
YC
Chen
Department of Cosmeceutics, China Medical University, Taichung 40402, Taiwan.
K J
KJ
Senthil Kumar
Department of Applied Chemistry, Chao Yang University of Technology, Taichung 41349, Taiwan.
Chee-Shan
CS
Chen
Institute of Environmental Health, China Medical University, Taichung 40402, Taiwan.
Ching-Tsan
CT
Tsai
School of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung 40402, Taiwan.
Hui-Chi
HC
Huang
Department of Fragrance and Cosmetic Science, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
Hui-Min
HM
Wang
Institute of Nutrition, China Medical University, Taichung 40421, Taiwan. Electronic address: hlyang@mail.cmu.edu.tw.
Hsin-Ling
HL
Yang
D000975:Antioxidants / Q000494:pharmacology*; D055491:Antrodia / Q000737:chemistry*; D002614:Chelating Agents / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D004912:Erythrocytes / Q000187:drug effects*; D006461:Hemolysis; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*
D000975:Antioxidants; D002614:Chelating Agents; D008077:Lipoproteins, LDL
Antioxidant; Antrodia salmonea; Atherosclerosis; Hemolysis; LDL