Conservation and function of Dazl in promoting the meiosis of goat male germline stem cells.
Dazl (deleted in azoospermia-like) is a conserved gene in mammalian meiosis, which encodes RNA binding protein required for spermatocyte meiosis. Up to date, the expression and function of Dazl in the goat testis are unknown. The objectives of this study were to investigate the expression pattern of Dazl in dairy goat testis and their function in male germline stem cells (mGSCs). The results first revealed that the expression level of Dazl in adult testes was significantly higher than younger and immature goats, and azoospermia and male intersex testis. The dairy goat Dazl is highly conserved analysed by several online and bioinformatics software, respectively. Over-expression of Dazl promoted the expression of meiosis-related genes in dairy goat mGSCs. The expression of Stra8 was up-regulated by over-expression of Dazl analysed by Luciferase reporter assay. Taken together, results suggest the Dazl plays an important role in dairy goat spermatogenesis and that over-expression of Dazl may promote Stra8 expression in dairy goat mGSCs.
College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Key Lab for Animal Biotechnology of Agriculture Ministry of China, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Zhiwei
Z
Niu
Yue
Y
Hu
Mingzhi
M
Liao
Meng
M
Yu
Haijing
H
Zhu
Long
L
Wang
Jiang
J
Wu
Chunling
C
Bai
Guangpeng
G
Li
Jinlian
J
Hua
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D015870:Gene Expression; D005854:Germ Cells / Q000378:metabolism*; D006041:Goats / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008540:Meiosis / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism*; D013737:Testis / Q000378:metabolism; D015533:Transcriptional Activation
D003062:Codon; D016601:RNA-Binding Proteins