Photobiocatalytic chemistry of oxidoreductases using water as the electron donor.
To date, water has been poorly studied as the sacrificial electron donor for biocatalytic redox reactions using isolated enzymes. Here we demonstrate that water can also be turned into a sacrificial electron donor to promote biocatalytic redox reactions. The thermodynamic driving force required for water oxidation is obtained from UV and visible light by means of simple titanium dioxide-based photocatalysts. The electrons liberated in this process are delivered to an oxidoreductase by simple flavin redox mediators. Overall, the feasibility of photobiocatalytic, water-driven bioredox reactions is demonstrated.
Instituto de Tecnología Química UPV-CSIC, Avda de los Naranjos s/n, Valencia 46022, Spain.
Maria
M
Mifsud
Department of Biotechnology, Delft University of Technology, Julianalaan 136, Delft 2628BL, The Netherlands.
Serena
S
Gargiulo
Instituto de Tecnología Química UPV-CSIC, Avda de los Naranjos s/n, Valencia 46022, Spain.
Sara
S
Iborra
Department of Biotechnology, Delft University of Technology, Julianalaan 136, Delft 2628BL, The Netherlands.
Isabel W C E
IW
Arends
Department of Biotechnology, Delft University of Technology, Julianalaan 136, Delft 2628BL, The Netherlands.
Frank
F
Hollmann
Instituto de Tecnología Química UPV-CSIC, Avda de los Naranjos s/n, Valencia 46022, Spain.
Avelino
A
Corma
D002384:Catalysis; D004583:Electrons; D007700:Kinetics; D010088:Oxidoreductases / Q000737:chemistry / Q000378:metabolism*; D010777:Photochemistry; D013816:Thermodynamics; D014867:Water / Q000737:chemistry*
D014867:Water; D010088:Oxidoreductases