Optimization of solid phase extraction clean up and validation of quantitative determination of carbazochrome sodium sulfonate in human plasma by liquid chromatography-electrospray ionization tandem mass spectrometry.
A mixed-mode anion exchange solid phase extraction (SPE) method for extraction and clean up of carbazochrome sodium sulfonate (CSS) and (1S)-(+)-10-camphorsulfonic acid (IS) was optimized for quantification by high-performance liquid chromatography/negative electrospray ionization mass spectrometry. The analytes were extracted from 1mL of human plasma via SPE on Oasis(®) WAX cartridge. Chromatographic separation was achieved on a Zorbax SB-Aq (4.6×250mm, 5μm) column under an isocratic condition. Detection was performed using electrospray ionization in negative ion multiple reaction monitoring (MRM) mode. The deprotonated precursor to product ion transitions monitored for CSS and IS was at m/z 299.0→256.0 and m/z 230.9→79.8, respectively. The method was fully validated for its selectivity, sensitivity, precision, accuracy, recovery, matrix effect and stability. Linear range was 0.189-37.8ng/mL with a high square regression coefficient (r=0.9995). The intra-and inter-day precision (RSD, %) ranged from 0.95% to 4.17%, and the intra-and inter-day accuracy was between 95.03% and 105.9%. This method was successfully applied to a bioequivalence study of 90mg CSS formulation in 18 healthy Chinese male subjects under fasting condition.
Research Center for Clinical Pharmacy, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, first Affiliated Hospital, Zhejiang University, Hangzhou, 310003, PR China.
Xingjiang
X
Hu
Research Center for Clinical Pharmacy, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, first Affiliated Hospital, Zhejiang University, Hangzhou, 310003, PR China.
Yunliang
Y
Zheng
Research Center for Clinical Pharmacy, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, first Affiliated Hospital, Zhejiang University, Hangzhou, 310003, PR China.
Guolan
G
Wu
Research Center for Clinical Pharmacy, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, first Affiliated Hospital, Zhejiang University, Hangzhou, 310003, PR China.
Jian
J
Liu
Research Center for Clinical Pharmacy, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, first Affiliated Hospital, Zhejiang University, Hangzhou, 310003, PR China.
Meixiang
M
Zhu
Research Center for Clinical Pharmacy, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, first Affiliated Hospital, Zhejiang University, Hangzhou, 310003, PR China.
Huili
H
Zhou
Research Center for Clinical Pharmacy, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, first Affiliated Hospital, Zhejiang University, Hangzhou, 310003, PR China.
You
Y
Zhai
Research Center for Clinical Pharmacy, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, first Affiliated Hospital, Zhejiang University, Hangzhou, 310003, PR China.
Lihua
L
Wu
Research Center for Clinical Pharmacy, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, first Affiliated Hospital, Zhejiang University, Hangzhou, 310003, PR China. Electronic address: stjz@zju.edu.cn.
Jianzhong
J
Shen-Tu
D000323:Adrenochrome / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification / Q000493:pharmacokinetics; D002853:Chromatography, Liquid / Q000379:methods; D018592:Cross-Over Studies; D004355:Drug Stability; D006801:Humans; D016014:Linear Models; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
D000323:Adrenochrome; C073338:carbazochrome
Carbazochrome sodium sulfonate; Human plasma; LC-ESI-MS/MS; Solid phase extraction