Isolation of Fungal Pathogens to an Edible Mushroom, Pleurotus eryngii, and Development of Specific ITS Primers.
Fungal pathogens have caused severe damage to the commercial production of Pleurotus eryngii, the king oyster mushroom, by reducing production yield, causing deterioration of commercial value, and shortening shelf-life. Four strains of pathogenic fungi, including Trichoderma koningiopsis DC3, Phomopsis sp. MP4, Mucor circinelloides MP5, and Cladosporium bruhnei MP6, were isolated from the bottle culture of diseased P. eryngii. A species-specific primer set was designed for each fungus from the ITS1-5.8S rDNA-ITS2 sequences. PCR using the ITS primer set yielded a unique DNA band for each fungus without any cross-reaction, proving the validity of our method in detection of mushroom fungal pathogens.
Department of Microbiology and Research Institute of Life Sciences, Gyeongsang National University, Jinju 660-771, Korea.
Sang-Woo
SW
Kim
Department of Microbiology and Research Institute of Life Sciences, Gyeongsang National University, Jinju 660-771, Korea.
Sinil
S
Kim
Department of Microbiology and Research Institute of Life Sciences, Gyeongsang National University, Jinju 660-771, Korea.
Hyun-Jun
HJ
Lee
Department of Microbiology and Research Institute of Life Sciences, Gyeongsang National University, Jinju 660-771, Korea.
Ju-Wan
JW
Park
Department of Microbiology and Research Institute of Life Sciences, Gyeongsang National University, Jinju 660-771, Korea.
Hyeon-Su
HS
Ro
Fungal pathogens; ITS primer; Mushroom; Pleurotus eryngii