Reduced expression of Phospholipase C beta in hippocampal interneuron during pilocarpine induced status epilepticus in mice.
We investigated localization of Phospholipase C beta (PLCβ1 and PLCβ4) in laminaes of dorsal hippocampus and different subtypes of hippocampal interneurons in normal Kunming mouse, and their progressive changes during pilocarpine induced status epilepticus (SE) by quantitative immunohistochemistry and real time PCR. PLCβ1 was observed in the pyramidal layer of CA1-3 area, hilus of the dentate gyrus, whereas PLCβ4 was mainly expressed in calcium binding protein positive interneurons, i.e. calbindin, calretinin, parvalbumin positive interneurons in the strata oriens, radiatum of the CA area and hilus of the dentate gyrus. During pilocarpine induced SE, a temporary down-regulation of PLCβ4 in the interneurons of CA area at SE 30min, and a progressive reduction of PLCβ1/PLCβ4 in dentate hilar cells were demonstrated. These findings confirm and extend the regional specific distribution of PLCβ1 and PLCβ4 immunoreactivity in mouse hippocampus, and suggest that PLCβ1 and PLCβ4 may play an important role in maintenance of the status epilepticus.
Institute of Neurobiology, School of Basic Medicine, Xi'an Jiaotong University Health Science Center, Xi'an 710061, PR China.
Jian Xin
JX
Liu
Institute of Neurobiology, School of Basic Medicine, Xi'an Jiaotong University Health Science Center, Xi'an 710061, PR China.
Ming
M
Hu
Institute of Neurobiology, School of Basic Medicine, Xi'an Jiaotong University Health Science Center, Xi'an 710061, PR China.
Xin Lin
XL
Chen
Department of Anatomy and Histology, School of Basic Medicine, Xi'an Jiaotong University Health Science Center, Xi'an 710061, PR China.
Jie Hua
JH
Xu
Department of Anatomy and Histology, School of Basic Medicine, Xi'an Jiaotong University Health Science Center, Xi'an 710061, PR China.
Peng Bo
PB
Yang
Institute of Neurobiology, School of Basic Medicine, Xi'an Jiaotong University Health Science Center, Xi'an 710061, PR China.
Jian Shui
JS
Zhang
Institute of Neurobiology, School of Basic Medicine, Xi'an Jiaotong University Health Science Center, Xi'an 710061, PR China. Electronic address: liuy5599@mail.xjtu.edu.cn.
Yong
Y
Liu
D000818:Animals; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000502:physiology; D005260:Female; D006624:Hippocampus / Q000187:drug effects* / Q000201:enzymology; D007150:Immunohistochemistry / Q000379:methods; D007395:Interneurons / Q000201:enzymology*; D051379:Mice; D054799:Phospholipase C beta / Q000378:metabolism*; D010862:Pilocarpine / Q000494:pharmacology*; D013226:Status Epilepticus / Q000139:chemically induced / Q000201:enzymology*
D010862:Pilocarpine; D054799:Phospholipase C beta
Calcium binding protein; Epilepsy; Hippocampus; Phospholipase C beta; Status epilepticus