The effects of Phα1β, a spider toxin, calcium channel blocker, in a mouse fibromyalgia model.
This study investigated the effects of Phα1β, pregabalin and diclofenac using an animal model of fibromyalgia (FM). Repeated administration of reserpine (0.25 mg/kg sc) once daily for three consecutive days significantly decreased thermal hyperalgesia, mechanical allodynia, and dopamine and serotonin content in the brain on the 4th day. Phα1β and pregabalin treatment completely reverted the mechanical allodynia and thermal hyperalgesia induced by reserpine treatment on the 4th day, but diclofenac was ineffective. Reserpine treatment significantly increased the immobility time in the forced swim test, which is indicative of depression in the animals. Phα1β, but not pregabalin, reduced the immobility time (56%), suggesting that Phα1β may control persistent pathological pain in FM.
Programa de Pós-Graduação em Genética e Toxicologia Aplicada (PPGGTA), Universidade Luterana do Brasil (ULBRA), Canoas, RS, Brazil; Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada à Saúde (PPGBioSaúde), ULBRA, Canoas, RGS, Brazil.
Alessandra Hubner
AH
de Souza
Programa de Pós Graduação Biomedicina e Medicina, Instituto de Ensino e Pesquisa da Santa Casa, Belo Horizonte, MG, Brazil.
Aírton Martins
AM
da Costa Lopes
Programa de Pós Graduação Biomedicina e Medicina, Instituto de Ensino e Pesquisa da Santa Casa, Belo Horizonte, MG, Brazil.
Célio J
CJ
Castro
Programa de Pós Graduação em Farmacologia Bioquimica e Molecular, Faculdade Medicina UFMG, Belo Horizonte, MG, Brazil.
Elizete Maria Rita
EM
Pereira
Programa de Pos Graduação em Biologia Celular e Molecular da Pontificia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, RGS, Brazil.
Caroline Peres
CP
Klein
Programa de Pós Graduação Biomedicina e Medicina, Instituto de Ensino e Pesquisa da Santa Casa, Belo Horizonte, MG, Brazil.
Claudio Antonio
CA
da Silva
Programa de Pós Graduação em Farmacologia Bioquimica e Molecular, Faculdade Medicina UFMG, Belo Horizonte, MG, Brazil.
Juliana Figueira
JF
da Silva
Programa de Pós Graduação em Farmacologia UFSM, Santa Maria, RGS, Brazil.
Juliano
J
Ferreira
Programa de Pós Graduação Biomedicina e Medicina, Instituto de Ensino e Pesquisa da Santa Casa, Belo Horizonte, MG, Brazil; Programa de Pós Graduação em Farmacologia Bioquimica e Molecular, Faculdade Medicina UFMG, Belo Horizonte, MG, Brazil. Electronic address: marcusvgomez@gmail.com.
Marcus Vinicius
MV
Gomez
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology / Q000627:therapeutic use*; D004008:Diclofenac / Q000494:pharmacology / Q000627:therapeutic use; D004195:Disease Models, Animal; D004298:Dopamine / Q000378:metabolism; D005356:Fibromyalgia / Q000188:drug therapy*; D006930:Hyperalgesia / Q000139:chemically induced / Q000188:drug therapy*; D008297:Male; D051379:Mice; D010146:Pain / Q000188:drug therapy; D000069583:Pregabalin; D012110:Reserpine; D012701:Serotonin / Q000378:metabolism; D013111:Spider Venoms / Q000494:pharmacology / Q000627:therapeutic use*; D013112:Spiders; D005680:gamma-Aminobutyric Acid / Q000031:analogs & derivatives / Q000494:pharmacology / Q000627:therapeutic use
D002121:Calcium Channel Blockers; D013111:Spider Venoms; D004008:Diclofenac; D012701:Serotonin; D000069583:Pregabalin; D005680:gamma-Aminobutyric Acid; D012110:Reserpine; D004298:Dopamine
Diclofenac; Fibromyalgia model; Phα1β; Pregabalin; Treatment