[Human β-defensin-3 regulates the proliferation and the secretion of prostaglandin E2 and matrix metalloproteinase-1 in human gingival fibroblasts].
OBJECTIVE
To observe the effect of human β-defensin-3 (HBD-3) on proliferation and the secretion of prostaglandin E2 (PGE2) and matrix metalloproteinase-1(MMP-1) in human gingival fibroblasts(HGF).


METHODS
The HGF were cultured with tissue-explant method and the fourth-generation HGF were plated in 96-well plate. All groups except the control group were treated with different concentrations of HBD-3 for 7 days. Then the HGF proliferation was evaluated with methyl thiazolyl tetrazolium(MTT) colorimetry and the secretions of PGE2 and MMP-1 at the 12th hours of each group were detected by enzyme-linked immunosorbent assay (ELISA).


RESULTS
The result of MTT dynamic monitoring showed that the amount of HGF increased with time in all groups in concentration dependent manner.ELISA showed that the secretions of PGE2 and MMP-1 in 1.0 mg/L HBD-3 group were (350.56 ± 63.96) ng/L and (13.22 ± 0.59) µg/L, significantly higher than those in the control group and 10.0 mg/L HBD-3 group (P < 0.05).


CONCLUSIONS
HBD-3 promoted the proliferation of HGF. The low concentration of HBD-3 may play a role in immunoregulation through increasing the secretions of PGE2 and MMP-1.
Department of Periondontics, Stomatological Hospital, Tianjin Medical University, Tianjin 300070, China.
Chen-xin
CX
Jin
Zhi-xiang
ZX
Qiao
Bing
B
Liu
Dong-lai
DL
Chen
Email: wangyonglan999@tom.com.
Yong-lan
YL
Wang
D000293:Adolescent; D000328:Adult; D000890:Anti-Infective Agents / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D015232:Dinoprostone / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005881:Gingiva / Q000166:cytology / Q000378:metabolism; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism*; D055815:Young Adult; D023083:beta-Defensins / Q000494:pharmacology*
D000890:Anti-Infective Agents; C422950:DEFB103A protein, human; D023083:beta-Defensins; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1; D015232:Dinoprostone