Acerola (Malpighia emarginata DC.) juice intake protects against alterations to proteins involved in inflammatory and lipolysis pathways in the adipose tissue of obese mice fed a cafeteria diet.
BACKGROUND
Obesity has been studied as a metabolic and an inflammatory disease and is characterized by increases in the production of pro-inflammatory adipokines in the adipose tissue.To elucidate the effects of natural dietary components on the inflammatory and metabolic consequences of obesity, we examined the effects of unripe, ripe and industrial acerola juice (Malpighia emarginata DC.) on the relevant inflammatory and lipolysis proteins in the adipose tissue of mice with cafeteria diet-induced obesity.


METHODS
Two groups of male Swiss mice were fed on a standard diet (STA) or a cafeteria diet (CAF) for 13 weeks. Afterwards, the CAF-fed animals were divided into five subgroups, each of which received a different supplement for one further month (water, unripe acerola juice, ripe acerola juice, industrial acerola juice, or vitamin C) by gavage. Enzyme-linked immunosorbent assays, Western blotting, a colorimetric method and histology were utilized to assess the observed data.


RESULTS
The CAF water (control obese) group showed a significant increase in their adiposity indices and triacylglycerol levels, in addition to a reduced IL-10/TNF-α ratio in the adipose tissue, compared with the control lean group. In contrast, acerola juice and Vitamin C intake ameliorated the weight gain, reducing the TAG levels and increasing the IL-10/TNF-α ratio in adipose tissue. In addition, acerola juice intake led to reductions both in the level of phosphorylated JNK and to increases in the phosphorylation of IκBα and HSLser660 in adipose tissue.


CONCLUSIONS
Taken together, these results suggest that acerola juice reduces low-grade inflammation and ameliorates obesity-associated defects in the lipolytic processes.
Fernando Milanez
FM
Dias
Daniela Dimer
DD
Leffa
Francine
F
Daumann
Schérolin de Oliveira
Sde O
Marques
Thais F
TF
Luciano
Jonathan Correa
JC
Possato
Aline Alves
AA
de Santana
Rodrigo Xavier
RX
Neves
José Cesar
JC
Rosa
Lila Missae
LM
Oyama
Bruno
B
Rodrigues
Vanessa Moraes
VM
de Andrade
Cláudio Teodoro
CT
de Souza
Immunometabolism Research Group, Department of Physical Education, Universidade Estadual Paulista, UNESP, Rua Roberto Simonsen, 305, 19060-900 Presidente Prudente, SP, Brazil. fabiolira@fct.unesp.br.
Fabio Santos
FS
de Lira
D000284:Administration, Oral; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D001205:Ascorbic Acid / Q000008:administration & dosage; D016207:Cytokines / Q000378:metabolism*; D004032:Diet; D004353:Drug Evaluation, Preclinical; D002149:Energy Intake; D004822:Epididymis / Q000378:metabolism / Q000473:pathology; D005638:Fruit / Q000737:chemistry; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D050152:Intra-Abdominal Fat / Q000378:metabolism*; D008066:Lipolysis; D008297:Male; D029581:Malpighiaceae / Q000737:chemistry*; D051379:Mice; D008820:Mice, Obese; D009765:Obesity; D010936:Plant Extracts / Q000008:administration & dosage*
D000893:Anti-Inflammatory Agents; D016207:Cytokines; D018836:Inflammation Mediators; D010936:Plant Extracts; D001205:Ascorbic Acid