Novel phage display-derived H5N1-specific scFvs with potential use in rapid avian flu diagnosis.
The highly pathogenic avian influenza A (HPAI) viruses of the H5N1 subtype infect poultry and have also been spreading to humans. Although new antiviral drugs and vaccinations can be effective, rapid detection would be more efficient to control the outbreak of infections. In this study, a phage-display library was applied to select antibody fragments for HPAI strain A/Hubei/1/2010. As a result, three clones were selected and sequenced. A hemagglutinin inhibition assay of the three scFvs revealed that none exhibited hemagglutination inhibition activity towards the H5N1 virus, yet they showed a higher binding affinity for several HPAI H5N1 strains compared with other influenza viruses. An ELISA confirmed that the HA protein was the target of the scFvs, and the results of a protein structure simulation showed that all the selected scFvs bound to the HA2 subunit of the HA protein. In conclusion, the three selected scFVs could be useful for developing a specific detection tool for the surveillance of HPAI epidemic strains.
Center for Disease Control and Prevention, Guangdong Province 510444, P. R. China.
Jie
J
Wu
Xian-Qiao
XQ
Zeng
Hong-Bin
HB
Zhang
Han-Zhong
HZ
Ni
Lei
L
Pei
Li-Rong
LR
Zou
Li-Jun
LJ
Liang
Xin
X
Zhang
Jin-Yan
JY
Lin
Chang-Wen
CW
Ke
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity / Q000276:immunology*; D061505:Cell Surface Display Techniques; D002645:Chickens; D006385:Hemagglutination Inhibition Tests; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D005585:Influenza in Birds; D007251:Influenza, Human / Q000175:diagnosis* / Q000276:immunology*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D019151:Peptide Library; D011485:Protein Binding / Q000276:immunology; D011487:Protein Conformation; D057127:Single-Chain Antibodies / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D019151:Peptide Library; D057127:Single-Chain Antibodies