Single-point mutation detection in RNA extracts using gold nanoparticles modified with hydrophobic molecular beacon-like structures.
Gold nanoparticles functionalized with oligonucleotides that bear a cholesterol group are used as gene sensors. The hydrophobic molecule is buried inside the nanostructure but when the complementary RNA sequence is present the structure unfolds exposing the cholesterol group to the water molecules. This rearrangement leads to the aggregation of the nanostructures.
Instituto Madrileño de Estudios Avanzados en Nanociencia (IMDEA Nanociencia), & CNB-CSIC-IMDEA Nanociencia Associated Unit "Unidad de Nanobiotecnología" Cantoblanco, 28049 Madrid, Spain. alvaro.somoza@imdea.org.
Alfonso
A
Latorre
Christian
C
Posch
Yolanda
Y
Garcimartín
Susana
S
Ortiz-Urda
Álvaro
Á
Somoza
D000818:Animals; D015374:Biosensing Techniques; D002460:Cell Line; D002784:Cholesterol / Q000031:analogs & derivatives; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry* / Q000235:genetics; D017354:Point Mutation; D012313:RNA / Q000235:genetics* / Q000302:isolation & purification
D009841:Oligonucleotides; D012313:RNA; D006046:Gold; D002784:Cholesterol