Similar in vitro effects and pulp regeneration in ectopic tooth transplantation by basic fibroblast growth factor and granulocyte-colony stimulating factor.
OBJECTIVE
Granulocyte-colony stimulating factor (G-CSF) has been shown to have combinatorial trophic effects with dental pulp stem cells for pulp regeneration. The aim of this investigation is to examine the effects of basic fibroblast growth factor (bFGF) in vitro and in vivo compared with those of G-CSF and to assess the potential utility of bFGF as an alternative to G-CSF for pulp regeneration.


METHODS
Five different types of cells were examined in the in vitro effects of bFGF on cell migration, proliferation, anti-apoptosis, neurite outgrowth, angiogenesis, and odontogenesis compared with those of G-CSF. The in vivo regenerative potential of pulp tissue including vasculogenesis and odontoblastic differentiation was also compared using an ectopic tooth transplantation model.


RESULTS
Basic fibroblast growth factor was similar to G-CSF in high migration, proliferation and anti-apoptotic effects and angiogenic and neurite outgrowth stimulatory activities in vitro. There was no significant difference between bFGF and G-CSF in the regenerative potential in vivo.


CONCLUSIONS
The potential utility of bFGF for pulp regeneration is demonstrated as a homing/migration factor similar to the influence of G-CSF.
Department of Dental Regenerative Medicine, Center of Advanced Medicine for Dental Oral Diseases, National Center for Geriatrics and Gerontology, Research Institute, Obu, Japan; Department of Endodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
N
N
Takeuchi
Y
Y
Hayashi
M
M
Murakami
F J
FJ
Alvarez
H
H
Horibe
K
K
Iohara
K
K
Nakata
H
H
Nakamura
M
M
Nakashima
D000293:Adolescent; D000328:Adult; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D003782:Dental Pulp / Q000187:drug effects / Q000502:physiology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D016513:Mice, SCID; D008963:Molar / Q000502:physiology / Q000637:transplantation*; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000502:physiology; D009805:Odontogenesis / Q000187:drug effects / Q000502:physiology; D013552:Swine; D055815:Young Adult
D016222:Fibroblast Growth Factor 2; D016179:Granulocyte Colony-Stimulating Factor
basic fibroblast growth factor; granulocyte-colony stimulating factor; homing/migration factor; pulp regeneration