The atopic skin-like microenvironment modulates the T-cell-polarising cytokine production of myeloid dendritic cells, as determined by laser scanning cytometry.
Because it is not known exactly when or where myeloid dendritic cells (mDCs) acquire their atopic dermatitis (AD)-specific T-cell-polarising ability in patients with this condition, we used laser scanning cytometry (LSC) to determine whether isolated peripheral blood mDCs from AD patients differed from cells from controls in their cytokine expression profiles de novo and after stimulation with Staphylococcus enterotoxin B (SEB) and thymic stromal lymphopoietin (TSLP), which represents an AD-like microenvironment. Unstimulated mDCs from AD patients showed pluripotent T-cell-polarising capacity, and the surrounding skin microenvironment was essential for the distinctive, disease-specific activity of mDCs (Th2-Th22 bias). We also emphasise that LSC is an attractive technique to study the effect of new DC-targeted therapeutic modalities in AD.
Department of Dermatology, University of Debrecen Medical and Health Science Centre, Debrecen, Hungary; Department of Dermatological Allergology, University of Debrecen Medical and Health Science Centre, Debrecen, Hungary.
Georgina
G
Nagy
Quang-Minh
QM
Doan-Xuan
Krisztián
K
Gáspár
Gábor
G
Mócsai
Anikó
A
Kapitány
Dániel
D
Törőcsik
Zsolt
Z
Bacsó
Edit
E
Gyimesi
Eva
E
Remenyik
Tamás
T
Bíró
Andrea
A
Szegedi
D016022:Case-Control Studies; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D003713:Dendritic Cells / Q000378:metabolism*; D003876:Dermatitis, Atopic / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D047569:Laser Scanning Cytometry; D022423:Myeloid Cells / Q000378:metabolism*
D016207:Cytokines
atopic dermatitis; laser scanning cytometry; myeloid dendritic cells