Teardrop shapes minimize bending energy of fusion pores connecting planar bilayers.
A numerical gradient flow procedure was devised to characterize minimal energy shapes of fusion pores connecting two parallel planar bilayer membranes. Pore energy, composed of splay, tilt, and stretching, was obtained by modeling each bilayer as two monolayers and treating each monolayer of a bilayer membrane as a freely deformable surface described with a mean lipid orientation field. Voids between the two monolayers were prevented by a steric penalty formulation. Pore shapes were assumed to possess both axial and reflectional symmetry. For fixed pore radius and bilayer separation, the gradient flow procedure was applied to initially toroidal pore shapes. Using initially elliptical pore shapes yielded the same final shape. The resulting minimal pore shapes and energies were analyzed as a function of pore dimension and lipid composition. Previous studies either assumed or confined pore shapes, thereby tacitly supplying an unspecified amount of energy to maintain shape. The shapes derived in the present study were outputs of calculations and an externally provided energy was not supplied. Our procedure therefore yielded energy minima significantly lower than those reported in prior studies. The membrane of minimal energy pores bowed outward near the pore lumen, yielding a pore length that exceeded the distance between the two fusing membranes.
Department of Mathematics, Fordham University, 441 E. Fordham Road, Bronx, New York 10458, USA.
Rolf J
RJ
Ryham
Department of Mathematics, Fordham University, 441 E. Fordham Road, Bronx, New York 10458, USA.
Mark A
MA
Ward
Department of Molecular Biophysics and Physiology, Rush University Medical Center, 1750 W. Harrison St., Chicago, Illinois 60612, USA.
Fredric S
FS
Cohen
D001696:Biomechanical Phenomena; D002462:Cell Membrane / Q000378:metabolism*; D008051:Lipid Bilayers / Q000378:metabolism*; D055595:Mechanical Phenomena; D008561:Membrane Fusion; D008954:Models, Biological; D016062:Porosity; D013816:Thermodynamics
D008051:Lipid Bilayers