Prooxidative-antioxidative balance of cells in different types of renal replacement therapy.
BACKGROUND
Patients suffering from chronic kidney disease (CKD) are exposed to increased oxidative stress and disturbances manifesting in the enzymatic and non-enzymatic antioxidative defence system. The object of the research was to assess the differences between conservative treatment, peritoneal dialysis and haemodialysis in moderating cellular antioxidative agents.


METHODS
The group examined comprised 145 patients. The activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glucose-6-phosphate dehydrogenase were obtained using kinetic methods. The spectrophotometric method established the concentrations of reduced glutathione, albumin, uric acid, glucose, total protein and lipids.


RESULTS
The type of treatment determined significant changes in antioxidative enzyme activities and concentrations of non-enzymatic antioxidative compounds.


CONCLUSIONS
Peritoneal dialysis provides better antioxidant protection than other types of therapy in CKD and should be considered as first-choice treatment despite more metabolic disorders.
Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University, Szczecin, Poland.
Joanna
J
Stępniewska
Barbara
B
Dołęgowska
Marlena
M
Popińska
Daria
D
Sałata
Marta
M
Budkowska
Edyta
E
Gołembiewska
Marek
M
Myślak
Maciej
M
Domański
Małgorzata
M
Marchelek-Myśliwiec
Kazimierz
K
Ciechanowski
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005978:Glutathione / Q000097:blood / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D006435:Renal Dialysis / Q000379:methods*; D051436:Renal Insufficiency, Chronic / Q000097:blood / Q000378:metabolism* / Q000473:pathology / Q000628:therapy*; D012709:Serum Albumin / Q000032:analysis; D013482:Superoxide Dismutase / Q000378:metabolism; D014527:Uric Acid / Q000097:blood
D000975:Antioxidants; D008055:Lipids; D012709:Serum Albumin; D014527:Uric Acid; D005954:Glucosephosphate Dehydrogenase; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005978:Glutathione