Arsenic trioxide targets miR-125b in glioma cells.
BACKGROUND
Arsenic trioxide (As2O3) has been demonstrated to suppress tumorigenesis in human glioma. However, the exact molecular mechanisms by which As2O3 exerts its tumor suppressor functions are elusive. Therefore, it is warranted to explore the underlying mechanism of As2O3-mediated anti-tumor activity in glioma.


METHODS
To achieve our goal, we used multiple approaches including MTT assay, apoptosis, Real-time RT-PCR, Western blotting, invasion assay, and gene transfection.


RESULTS
We observed that A22O3 inhibited cell growth and induced apoptosis as well as suppressed migration and invasion in human glioma cells. Moreover, we found that As2O3 down-regulated miR-125b expression and subsequently up-regulated its target gene Bak1 expression. Furthermore, we identified that As2O3 exerts its anti-tumor activity partly through regulation of miR-125b.


CONCLUSIONS
Our present study suggests that As2O3 could be a potential therapeutic agent for treatment of human glioma.
Sulian
S
Chen
Lihua
L
Zhu
Jing
J
Huang
Ying
Y
Cai
Xiaohui
X
Lu
Qingling
Q
Yang
Qiong
Q
Wu
Changjie
C
Chen
Department of Biochemistry and Molecular Biology, Bengbu Medical College, Anhui 233030, China. tochenchangjie@163.com.
Zhiwei
Z
Wang
D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000737:chemistry / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D005910:Glioma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D010087:Oxides / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D001152:Arsenicals; C531888:MIRN125 microRNA, human; D035683:MicroRNAs; D010087:Oxides; D000077237:Arsenic Trioxide