[Protective effect of isoorientin on alcohol-induced hepatic fibrosis in rats].
OBJECTIVE
To observe the effect and mechanism of isoorientin from Gypsophila elegans on alcohol-induced hepatic fibrosis in rats.


METHODS
ninety healthy male Wistar rats were randomly divided into six groups: the normal control group, the model control group, the colchicines group (positive control, 1.0 mg x kg(-1) x d(-1)), the high, middle and low-dose isoorientin groups (20, 50, 100 mg x kg(-1) x d(-1)). The normal control group received normal saline, while other groups received alcohol to cause hepatic fibrosis. After 24-weeks treatment, the alanine aminotransferase (ALT), aspartate aminotransferase (AST), Interleukin 6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), hyaluronic acid (HA), laminin (LN), type III precollagen (PCIII), hydroxyproline (Hyp), Myeloperoxidase (MPO), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) were assayed according to the manufacturer's instructions, the alpha-SMA and TGF-beta1 were detected by western blotting, and the histopathological changes was observed by H&E staining.


RESULTS
Isoorientin could improve the liver function by decreasing the activity of ALT, AST, IL-6, TNF-alpha, MDA, MPO, HA, LN, PCIII and Hyp (P < 0.05), increasing the activity of SOD and GSH-Px (P < 0.05), and reducing the expression of alpha-SMA and TGF-beta1 (P < 0.05). In addition, the high and middle-dose isoorientin groups showed more remarkable effect


CONCLUSIONS
Isoorientin from G. elegans can protect hepatic fibrosis induced by alcohol.
Guangxi Medical University, Nanning 530021, China. carolchen8811@163.com
Yong-Xin
YX
Chen
The First Affiliated Hospital of Guangxi Traditional Chinese Medicine University, Nanning 530023, China.
Quan-Fang
QF
Huang
Guangxi Medical University, Nanning 530021, China.
Xing
X
Lin
Guangxi Medical University, Nanning 530021, China.
Jin-Bin
JB
Wei
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D000431:Ethanol / Q000009:adverse effects; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000188:drug therapy / Q000201:enzymology / Q000517:prevention & control*; D047311:Luteolin / Q000008:administration & dosage*; D008297:Male; D020011:Protective Agents / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D020011:Protective Agents; D053773:Transforming Growth Factor beta1; D000431:Ethanol; C057912:homoorientin; D005979:Glutathione Peroxidase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D047311:Luteolin