Morphogenetic study on the maturation of osteoblastic cell as induced by inorganic polyphosphate.
Since inorganic polyphosphates [poly(P)] have an activity to induce bone differenciation in vitro and in vivo, we examined an effect of poly(P) on organelle by light microscopy and electron microscopy in Murine MC3T3-E1 osteoblastic cells. The MC3T3-E1 cells were ultrastructurally observed to possess morphological characteristics of osteoblasts. Cells cultured with poly(P) were strongly stained with an anti-collagen type I antibody but not in those cultured without poly(P). Ultrastructural analysis of cells cultured with poly(P) revealed a well-developed Golgi apparatus, swollen and elongated rough endoplasmic reticulum, large mitochondria and many coated pits. Since MC3T3-E1 cells can be transformed from a resting phase to an active blastic cell phase after supplementation with poly(P), it implies that poly(P) can be an effective material for bone regeneration.
Department of Biological Sciences and Engineering, Faculty of Health Sciences, Hokkaido University, Kita-ku, Sapporo, Japan.
Kaori
K
Tsutsumi
Department of Internal Medicine, Gastroenterology & Hematology Section, Hokkaido University Graduate School of Medicine, Kita-ku, Sapporo, Japan.
Nagahito
N
Saito
Regenetiss Inc., Kunitachi, Tokyo, Japan.
Yumi
Y
Kawazoe
Department of Veterinary Medicine, Azabu University, Chuo-ku, Sagamihara-Shi, Kanagawa, Japan.
Hong-Kean
HK
Ooi
Regenetiss Inc., Kunitachi, Tokyo, Japan ; Laboratory for Polyphosphate Research, The Kitasato Institute, Kitasato Institute for Life Sciences, Kitasato University, Minato-ku, Tokyo, Japan.
Toshikazu
T
Shiba
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D024042:Collagen Type I / Q000378:metabolism; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D051379:Mice; D046529:Microscopy, Electron, Transmission; D008858:Microscopy, Phase-Contrast; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D010012:Osteogenesis / Q000187:drug effects*; D053495:Osteopontin / Q000378:metabolism; D011122:Polyphosphates / Q000494:pharmacology*; D013997:Time Factors; D014617:Vacuoles / Q000187:drug effects / Q000648:ultrastructure
D024042:Collagen Type I; D011122:Polyphosphates; D053495:Osteopontin