3-dimensional tissue is formed from cancer cells in vitro on Gelfoam®, but not on Matrigel™.
Cell and tissue culture can be performed on different substrates such as on plastic, in Matrigel™, and on Gelfoam(®), a sponge matrix. Each of these substrates consists of a very different surface, ranging from hard and inflexible, a gel, and a sponge-matrix, respectively. Folkman and Moscona found that cell shape was tightly coupled to DNA synthesis and cell growth. Therefore, the flexibility of a substrate is important for cells to maintain their optimal shape. Human osteosarcoma cells, stably expressing a fusion protein of α(v) integrin and green fluorescent protein (GFP), grew as a simple monolayer without any structure formation on the surface of a plastic dish. When the osteosarcoma cells were cultured within Matrigel™, the cancer cells formed colonies but no other structures. When the cancer cells were seeded on Gelfoam(®), the cells formed three-dimensional tissue-like structures. The behavior of 143B osteosarcoma cells on Gelfoam(®) in culture is remarkably different from those of these cells in monolayer culture or in Matrigel™. Tissue-like structures were observed only in Gelfoam(®) culture. The data in this report suggest a flexible structural substrate such as Gelfoam(®) provides a more in vivo-like culture condition than monolayer culture or Matrigel(TM) and that Matrigel(TM) does not result in actual three-dimensional culture.
AntiCancer, Inc., 7917 Ostrow Street, San Diego, California, 92111; Department of Surgery, University of California San Diego, 200 West Arbor Drive, San Diego, California, 92103; Department of Orthopedic Surgery, Graduate School of Medicine, University of the Ryukyus, 207 Uehara Nishihara, Okinawa, 903-0125, Japan.
Yasunori
Y
Tome
Fuminari
F
Uehara
Sumiyuki
S
Mii
Shuya
S
Yano
Lei
L
Zhang
Naotoshi
N
Sugimoto
Hiroki
H
Maehara
Michael
M
Bouvet
Hiroyuki
H
Tsuchiya
Fuminori
F
Kanaya
Robert M
RM
Hoffman
D001859:Bone Neoplasms / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D003094:Collagen / Q000737:chemistry*; D004338:Drug Combinations; D005781:Gelatin Sponge, Absorbable / Q000737:chemistry*; D049452:Green Fluorescent Proteins; D006801:Humans; D066298:In Vitro Techniques; D039001:Integrin alpha Chains / Q000096:biosynthesis; D007797:Laminin / Q000737:chemistry*; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology; D011509:Proteoglycans / Q000737:chemistry*
D004338:Drug Combinations; D039001:Integrin alpha Chains; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D049452:Green Fluorescent Proteins; D003094:Collagen
CONFOCAL MICROSCOPY; CULTURE; DIMENSION; GELFOAM®; GREEN FLUORESCENT PROTEIN; HISTOLOGY; MATRIGEL™; OSTEOSARCOMA; REAL-TIME IMAGING; αv INTEGRIN