Quantitative analysis of phenolic metabolites from different parts of Angelica keiskei by HPLC-ESI MS/MS and their xanthine oxidase inhibition.
Angelica keiskei is used as popular functional food stuff. However, quantitative analysis of this plant's metabolites has not yet been disclosed. The principal phenolic compounds (1-16) within A. keiskei were isolated, enabling us to quantify the metabolites within different parts of the plant. The specific quantification of metabolites (1-16) was accomplished by multiple reaction monitoring (MRM) using a quadruple tandem mass spectrometer. The limit of detection and limit of quantitation were calculated as 0.4-44 μg/kg and 1.5-148 μg/kg, respectively. Abundance and composition of these metabolites varied significantly across different parts of plant. For example, the abundance of chalcones (12-16) decreased as follows: root bark (10.51 mg/g)>stems (8.52 mg/g)>leaves (2.63 mg/g)>root cores (1.44 mg/g). The chalcones were found to be responsible for the xanthine oxidase (XO) inhibition shown by this plant. The most potent inhibitor, xanthoangelol inhibited XO with an IC50 of 8.5 μM. Chalcones (12-16) exhibited mixed-type inhibition characteristics.
Division of Applied Life Science (BK21 Plus Program), IALS, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Dae Wook
DW
Kim
Graduate Program in Biochemistry and Biophysics, Brandeis University, 415 South Street, Waltham, MA 02453, USA.
Marcus J
MJ
Curtis-Long
Division of Applied Life Science (BK21 Plus Program), IALS, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Heung Joo
HJ
Yuk
Division of Applied Life Science (BK21 Plus Program), IALS, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Yan
Y
Wang
Division of Applied Life Science (BK21 Plus Program), IALS, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Yeong Hun
YH
Song
Namhae Garlic Research Institute, Namhae 668-812, Republic of Korea.
Seong Hun
SH
Jeong
Division of Applied Life Science (BK21 Plus Program), IALS, Gyeongsang National University, Jinju 660-701, Republic of Korea. Electronic address: khpark@gnu.ac.kr.
Ki Hun
KH
Park
D029969:Angelica / Q000737:chemistry* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004791:Enzyme Inhibitors / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D010636:Phenols / Q000032:analysis* / Q000378:metabolism; D010936:Plant Extracts / Q000032:analysis* / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014969:Xanthine Oxidase / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000378:metabolism
D004791:Enzyme Inhibitors; D010636:Phenols; D010936:Plant Extracts; D014969:Xanthine Oxidase
Angelica keiskei; Chalcone; Coumarin; MRM; Xanthine oxidase