[Study on fingerprint of shengxuening tablets].
To establish a method for studying fingerprint of Shengxuening tablets. With chlorin e6 as the reference substance, SHISEIDO Capcell-pak C18 (4.6 mm x 250 mm, 5 microm) analytical column was adopted and eluted with 0.2% formic acid ( containing 20 mmol x L(-1) TBAB) (A) and acetonitrile-methanol-acetone (50: 50: 5) (B). The detection wavelength was set 392 nm. The volume flow rate was 1.0 mL x min(-1). The temperature of column was 45 degrees C. Totally 10 common peaks were indicated on the HPLC fingerprint, with RSDs for variable retention values in common peaks below 0.50%. UPLC/DAD/Q-TOF-MS xevo G2 Q-TOF LC/MS was adopted to preliminarily indentify six chromatographic peaks. The main ingredient in Shengxuening tablets was ferrous derivative, which was mainly composed of Fe chlorin p6, Fe chlorin e6 and Fe isochlorin e4.
Hubei Institute for Food and Drug Control, Wuhan 430064, China. niejingwh@hotmail.com
Jing
J
Nie
Hubei Institute for Food and Drug Control, Wuhan 430064, China.
Hua-Sheng
HS
Hu
Hubei Institute for Food and Drug Control, Wuhan 430064, China.
Xiao-Yong
XY
Chen
Tongji Medical College of Huazhong University of Science & Technology, Wuhan 430030, China.
Juan-Juan
JJ
Liu
Hubei Institute for Food and Drug Control, Wuhan 430064, China.
Ke
K
Li
Wuhan United Pharmaceutical Co., Ltd., Wuhan 430073, China.
Jing
J
Luo
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D011786:Quality Control; D013607:Tablets / Q000032:analysis
D004365:Drugs, Chinese Herbal; D013607:Tablets; C516453:shengxuening