Beta-galactosidase staining in the skeleton.
The lacZ gene, encoding for the β-galactosidase enzyme, is widely used as a reporter gene in bone biology due to the ease of visualization in situ on whole-mount or on tissue sections. In this protocol we provide detailed methods for visualizing this reporter gene for both in vivo and in vitro studies. In addition, we describe a few tips for troubleshooting for mineralized tissues such as the skeleton.
Sichuan University, Chengdu, Sichuan, P.R. China.
X L
XL
Han
Baylor College of Dentistry, Dallas, TX, USA.
Jian Q
JQ
Feng
D000818:Animals; D001842:Bone and Bones / Q000166:cytology* / Q000201:enzymology*; D005333:Fetus; D006651:Histocytochemistry; D051379:Mice; D013194:Staining and Labeling; D001616:beta-Galactosidase / Q000378:metabolism*
D001616:beta-Galactosidase