Effect of high pressure processing on the conversion of dihydroxyacetone to methylglyoxal in New Zealand mānuka (Leptospermum scoparium) honey and models thereof.
The effect of high pressure processing (HPP) on the conversion of dihydroxyacetone (DHA) to methylglyoxal (MGO) was examined in New Zealand mānuka honey and models thereof. The objective was to confirm that previously reported increases of MGO with HPP treatment originated from conversion of DHA. RP-HPLC was used to quantify DHA, MGO and hydroxymethylfurfural (HMF) after derivatisation with O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine hydrochloride (PFBHA) or (in the case of MGO) separately with o-phenylenediamine (OPD). Fresh and stored mānuka honey, clover honey with DHA added and artificial 26 honey with DHA added were subjected to nine different pressures and holding times and compared to untreated samples. There was no consistent trend of decrease in DHA or increase in MGO for any of the samples with any treatment. Samples showed random change generally within 5-10% of an untreated sample for MGO, DHA and HMF. HPP does not accelerate the conversion of DHA to MGO in honey.
Department of Chemistry, University of Waikato, Private Bag 3105, Hamilton, New Zealand.
Megan N C
MN
Grainger
Department of Chemistry, University of Waikato, Private Bag 3105, Hamilton, New Zealand. Electronic address: manleyha@waikato.ac.nz.
Merilyn
M
Manley-Harris
Department of Chemical and Materials Engineering, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand.
Noor A M
NA
Fauzi
Department of Chemical and Materials Engineering, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand.
Mohammed M
MM
Farid
D004098:Dihydroxyacetone / Q000737:chemistry*; D005511:Food Handling / Q000295:instrumentation / Q000379:methods*; D006722:Honey / Q000032:analysis*; D031649:Leptospermum / Q000737:chemistry*; D008956:Models, Chemical; D009520:New Zealand; D011312:Pressure; D011765:Pyruvaldehyde / Q000737:chemistry*
D011765:Pyruvaldehyde; D004098:Dihydroxyacetone
Dihydroxyacetone; High pressure processing; Methylglyoxal; Mānuka honey