Nasal carriage of multi-drug resistant Panton-Valentine leucocidin-positive methicillin-resistant Staphylococcus aureus in children in Tripoli-Libya.
Methicillin-resistant Staphylococcus aureus (MRSA) colonized children are at an increased risk of developing infections than methicillin-sensitive S. aureus colonized children. Nasal specimens from inpatient children, mothers of inpatient children, healthcare workers, and outpatient children at Tripoli Children Hospital (TCH) were examined for MRSA by chromogenic MRSA ID medium. Susceptibility of MRSA isolates to antibiotics was determined by the disc diffusion method. The nasal carriage rate of MRSA among inpatient children (8.3%, 24 of 289), their mothers (11%, 22 of 200), and healthcare workers (12.4%, 22 of 178) was significantly higher than among outpatient children (2.2%, 2 of 91) (P < 0.05, P < 0.02, and P < 0.006, respectively). Of the examined MRSA isolates (N = 35) 10 (28.6%) were positive for Panton-Valentine leucocidin genes by polymerase chain reaction. Multidrug resistance was found in 24.3% (17 of 70) of MRSA isolates. Nasal carriage of multidrug-resistant Panton-Valentine leucocidin-positive MRSA is not uncommon among inpatient children and their mothers in Tripoli.
Faculty of Pharmacy and Faculty of Medicine, University of Tripoli, Tripoli, Libya.
Omaima H
OH
Al-haddad
Abdulaziz
A
Zorgani
Khalifa Sifaw
KS
Ghenghesh
D000293:Adolescent; D058345:Asymptomatic Infections / Q000453:epidemiology; D001427:Bacterial Toxins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D002353:Carrier State / Q000453:epidemiology* / Q000382:microbiology; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005098:Exotoxins / Q000235:genetics*; D005260:Female; D006282:Health Personnel; D006776:Hospitals, Pediatric; D006801:Humans; D007223:Infant; D007956:Leukocidins / Q000235:genetics*; D008002:Libya / Q000453:epidemiology; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D009035:Mothers; D009666:Nose / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000453:epidemiology* / Q000382:microbiology
D001427:Bacterial Toxins; D004269:DNA, Bacterial; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin