View synthesis with a partitioned-aperture microscope.
We present a simple and fast algorithm for view synthesis based on the acquisition of four high-resolution oblique images with a conventional widefield microscope. The images are acquired simultaneously using a partitioned aperture add-on. The technique provides physically valid views of thin samples that are transmitting or fluorescent, as demonstrated with biopsied tissue or green fluorescent protein-labeled brain slices. The goal of this technique is to facilitate image interpretation by conferring impressions of depth that are otherwise absent in standard microscope images.
J-C
JC
Baritaux
C R
CR
Chan
J
J
Li
J
J
Mertz
D000818:Animals; D001921:Brain / Q000166:cytology; D003106:Colon / Q000166:cytology; D007091:Image Processing, Computer-Assisted; D051379:Mice; D008853:Microscopy / Q000295:instrumentation*; D055620:Optical Phenomena