Isolation and identification ofVibrio parahaemolyticusfrom seawater and sediment samples in the southern coast of the Caspian Sea.
The objectives of this study were to investigate the occurrence ofVibrio parahaemolyticusin the seawater and its sediment by molecular techniques and conventional microbiological methods. Of 300 samples analyzed, 20.3 % was recorded positive forV. parahaemolyticus. Of the 62 strains isolated, 26 (8.3 %) were obtained from the seawater samples, and 36 (12 %); from sediments. Only three strains (4.83 %) showed hemolytic activity in Wagatsuma agar. The results of this study demonstrated the presence ofV. parahaemolyticusin the southern coast of the Caspian Sea (Northern Iran). Furthermore, the PCR approach proved useful for reliable confirmation of species identification.V. parahaemolyticusis an important human pathogen responsible for food-borne gastroenteritis worldwide. These findings indicated the potential sanitary risk associated with the presence of pathogenicV. parahaemolyticusin the Caspian Sea.
Department of Microbiology, Islamic Azad University (IAU), Babol Branch, Babol Iran.
Majid
M
Alipour
Department of Microbiology, Islamic Azad University (IAU), Lahyjan Branch, Lahyjan Iran.
Khosro
K
Issazadeh
Department of Microbiology, Islamic Azad University (IAU), Lahyjan Branch, Lahyjan Iran.
Javad
J
Soleimani
Acute gastroenteritis; Seawater; Sediment; Vibrio parahaemolyticus