Investigation of the gibberellic acid optimization with a statistical tool from Penicillium variable in batch reactor.
The culture conditions for gibberellic acid (GA3) production by the fungus Penicillium variable (P. variable) was optimized using a statistical tool, response surface methodology (RSM). Interactions of culture conditions and optimization of the system were studied using Box-Behnken design (BBD) with three levels of three variables in a batch flask reactor. Experimentation showed that the model developed based on RSM and BBD had predicted GA3 production with R(2) = 0.987. The predicted GA3 production was optimum (31.57 mg GA3/kg substrate) when the culture conditions were at 7 days of incubation period, 21% v/w of inoculum size, and 2% v/w of olive oil concentration as a natural precursor. The results indicated that RSM and BBD methods were effective for optimizing the culture conditions of GA3 production by P. variable mycelia.
a School of Chemical Engineering, Universiti Sains Malaysia, Engineering Campus, Seberang Perai Selatan , Penang , Malaysia.
Nur Kamilah Md
NK
Isa
Mashitah
M
Mat Don
D019149:Bioreactors; D003470:Culture Media / Q000737:chemistry*; D005163:Factor Analysis, Statistical; D005285:Fermentation; D005875:Gibberellins / Q000096:biosynthesis*; D000069463:Olive Oil; D010407:Penicillium / Q000378:metabolism*; D010938:Plant Oils / Q000378:metabolism
D003470:Culture Media; D005875:Gibberellins; D000069463:Olive Oil; D010938:Plant Oils; C007842:gibberellic acid