In vitro and in vivo photoprotective/photochemopreventive potential of Garcinia brasiliensis epicarp extract.
The damaging effects of sunlight to the skin has triggered studies that involve the synthesis and extraction of organic compounds from natural sources that can absorb UV radiation, and studies on polyphenolic compounds with antioxidant and anti-inflammatory properties that can be used as photochemopreventive agents for reducing skin damage. We investigated the in vitro and in vivo photoprotective/photochemopreventive potential of Garcinia brasiliensis epicarp extract (GbEE). We evaluated the cell viability of L929 fibroblasts after UVB exposure using a quartz plate containing the extract solution or the GbEE formulation. The in vivo photoprotective effect of the GbEE formulation was evaluated by measuring the UVB damage-induced decrease in endogenous reduced glutathione (GSH), the increase in myeloperoxidase (MPO) activity and secretion of cytokines IL-1β and TNF-α. The in vitro methodology using fibroblasts showed that the photoprotective properties of the GbEE solutions and 10% GbEE formulation were similar to the commercial sunscreen (SPF-15). In vivo results demonstrated of the GbEE formulation in decreasing UVB induced-damage such as GSH depletion, an increased in MPO activity and secretion of cytokines IL-1β and TNF-α. The results showed that the extract has great potential for use as a sunscreen in topical formulations in addition to UV filters.
Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Sônia Aparecida
SA
Figueiredo
Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Fernanda Maria Pinto
FM
Vilela
Laboratory of Phytochemistry and Medicinal Chemistry, Department of Pharmacy, Alfenas Federal University, Alfenas, Minas Gerais, Brazil.
Claudinei Alves
CA
da Silva
Department of Pharmacology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Thiago Mattar
TM
Cunha
Laboratory of Organic Chemistry, Department of Chemistry, Viçosa Federal University, Viçosa, Minas Gerais, Brazil.
Marcelo Henrique
MH
Dos Santos
Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. Electronic address: magika@fcfrp.usp.br.
Maria José Vieira
MJ
Fonseca
D000287:Administration, Topical; D000818:Animals; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects; D029760:Garcinia / Q000737:chemistry*; D005978:Glutathione / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D008812:Mice, Hairless; D009195:Peroxidase / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects / Q000528:radiation effects; D013472:Sunlight; D013473:Sunscreening Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000592:standards; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014466:Ultraviolet Rays
D053583:Interleukin-1beta; D010936:Plant Extracts; D013473:Sunscreening Agents; D014409:Tumor Necrosis Factor-alpha; D009195:Peroxidase; D005978:Glutathione
Anti-inflammatory; Antioxidant; Cytotoxicity; Garcinia brasiliensis; In vitro SPF; Photochemoprotection; Photoprotection