Site specific properties of carious dentin matrices biomodified with collagen cross-linkers.
OBJECTIVE
To assess in non-cavitated carious teeth the mechanical properties of dentin matrix by measuring its reduced modulus of elasticity and the effect of dentin biomodification strategies on three dentin matrix zones: caries-affected, apparently normal dentin below caries-affected zone and sound dentin far from carious site.


METHODS
Nano-indentations were performed on dentin matrices of carious molars before and after surface modification using known cross-linking agents (glutaraldehyde, proanthocyanidins from grape seed extract and carbodiimide).


RESULTS
Statistically significant differences were observed between dentin zones of demineralized dentin prior to surface biomodification (P < 0.05). Following surface modification, there were no statistically significant differences between dentin zones (P < 0.05). An average increase of 30-fold, 2-fold and 2.2-fold of the reduced modulus of elasticity was observed following treatments of the three dentin zones with proanthocyanidin, carbodiimide and glutaraldehyde, respectively.
Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, Room #551, 801 South Paulina Street, Chicago, IL 60612, USA. bedran@uic.edu
Ana K
AK
Bedran-Russo
Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, Room #551, 801 South Paulina Street, Chicago, IL 60612, USA.
Sachin
S
Karol
Department of Oral Biology, School of Dental Medicine, Georgia Health Sciences University, Augusta, Georgia, USA.
David H
DH
Pashley
Department of Orthodontics, College of Dentistry, Chicago, Illinois 60612, USA.
Grace
G
Viana
D003094:Collagen / Q000187:drug effects*; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D003731:Dental Caries / Q000473:pathology* / Q000503:physiopathology; D003804:Dentin / Q000187:drug effects* / Q000473:pathology; D055119:Elastic Modulus; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000494:pharmacology; D005976:Glutaral / Q000494:pharmacology; D056604:Grape Seed Extract / Q000494:pharmacology; D006244:Hardness; D006801:Humans; D020527:Microscopy, Scanning Probe; D008963:Molar / Q000187:drug effects / Q000473:pathology; D044945:Proanthocyanidins / Q000494:pharmacology; D017001:Tooth Demineralization / Q000473:pathology / Q000503:physiopathology; D027843:Vitis
D003432:Cross-Linking Reagents; D056604:Grape Seed Extract; C511402:Grape Seed Proanthocyanidins; D044945:Proanthocyanidins; D003094:Collagen; D005022:Ethyldimethylaminopropyl Carbodiimide; D005976:Glutaral