Expression of selected cytochrome P450 isoforms and of cooperating enzymes in colorectal tissues in selected pathological conditions.
The current interest in CYP expression in the colon results from its uniqueness as a target organ for cancer. To date, the CYP expression profiles in the colon have not yet been subject of comprehensive research. In this study, we investigated 40 patients with Crohn's disease, 40 with ulcerative colitis, and 40 healthy subjects as a control group. Colon tissues were fixed, dehydrated, cleared in xylene and embedded in paraffin. Sections were prepared from paraffin blocks for immunohistochemical staining with specific antibodies. We used antibodies to the human CYP1A1, CYP2B6, CYP2C9, CYP2E1 and CYP3A4 isoforms, as well as antibodies to the human glycoprotein P, glutathione-S transferase and antibody to the UDP-glucuronosyltransferase. The sections were stained immunohistochemically and examined using light microscopy. Cellular localization was determined, and computer image analysis was used. In all cases with Crohn's disease, the proteins studied showed at least a twofold expression. Ulcerative colitis showed a much weaker influence regarding the expression of the proteins studied but in case of CYP2C9 and UDP-glucuronosyltransferase, a decrease of expression was observed.
Department of Histology, Medical University of Silesia, Katowice, Poland.
Danuta
D
Plewka
Department of Proteomics, Medical University of Silesia, Sosnowiec, Poland. Electronic address: aplewka@sum.edu.pl.
Andrzej
A
Plewka
Department of Proteomics, Medical University of Silesia, Sosnowiec, Poland.
Tomasz
T
Szczepanik
Department of Proteomics, Medical University of Silesia, Sosnowiec, Poland.
Michał
M
Morek
Department of Proteomics, Medical University of Silesia, Sosnowiec, Poland.
Edyta
E
Bogunia
Department of Proteomics, Medical University of Silesia, Sosnowiec, Poland.
Piotr
P
Wittek
Department of Proteomics, Medical University of Silesia, Sosnowiec, Poland.
Czarosław
C
Kijonka
D000328:Adult; D003093:Colitis, Ulcerative / Q000201:enzymology*; D003424:Crohn Disease / Q000201:enzymology*; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis / Q000096:biosynthesis; D008297:Male; D008875:Middle Aged; D059467:Transcriptome
D007527:Isoenzymes; D003577:Cytochrome P-450 Enzyme System
Colorectal tissues; Crohn's disease; Cytochrome P450; Ulcerative colitis