Median lethal needle caliber in two models of experimental sepsis.
OBJECTIVE
To estimate the median lethal needle caliber (LC50) of a new experimental sepsis model and compare it to the LC50 of the cecal ligation and puncture (CLP) sepsis model.


METHODS
Male albino Wistar rats were studied (n=22). Animals were allocated into two study groups. In Group I, experimental sepsis was induced by cecal ligation and puncture. In Group II, experimental sepsis was induced by ascending colon ligation and cecal puncture. Up-and-down method was used to determinate the LC50.


RESULTS
LC50 in Group I was 19 Gauge (Confidence Interval 17 to 22 Gauge). Determination of LC50 was not possible in Group II due to the death of all animals.


CONCLUSIONS
LC50 in cecal ligation and puncture is 19 Gauge. The lethality of the new model tested in this trial is very high.
Federal University of Santa Catarina, Department of Surgery, FlorianopolisSC, Brazil.
Armando José
AJ
d'Acampora
UFSC, Department of Surgery, Division of Anesthesia, FlorianopolisSC, Brazil.
Giovani de Figueiredo
Gde F
Locks
D000818:Animals; D002432:Cecum / Q000293:injuries*; D044682:Colon, Ascending / Q000293:injuries*; D004195:Disease Models, Animal; D004867:Equipment Design; D008026:Ligation; D008297:Male; D009339:Needles; D011677:Punctures / Q000295:instrumentation*; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000209:etiology* / Q000401:mortality; D016019:Survival Analysis