Comparison of in vitro and in vivo bioactivity: cuttlefish-bone-derived hydroxyapatite and synthetic hydroxyapatite granules as a bone graft substitute.
Bone reconstruction in clinical settings often requires bone substitutes. Hydroxyapatite (HAp) is a widely used bone substitute due to its osteoconductive properties and bone bonding ability. The aim of this study was to evaluate HAp granules derived from cuttlefish bone (CB-HAp) as a substitute biomaterial for bone grafts. In this study, HAp granules were prepared from raw CB by using a hydrothermal reaction. The formation of HAp from CB was confirmed by scanning electron microscopy and x-ray diffraction analysis. The bioactivity of the CB-HAp granules was evaluated both in vitro and in vivo. Our results show that CB-HAp is non-toxic and that CB-HAp granules supported improved cell adhesion, proliferation and differentiation compared to stoichiometric synthetic HAp granules. Furthermore, in vivo bone defect healing experiments show that the formation of bone with CB-HAp is higher than that with pure HAp. These results show that CB-HAp granules have excellent potential for use as a bone graft material.
Wonkwang Bone Regeneration Research Institute, Wonkwang University, Iksan 570-749, Korea. Bonecell Biotech, Inc., 77, Dunsan-ro, Seo-gu, Daejeon 302-830, Korea.
Beom-Su
BS
Kim
Hyo Jin
HJ
Kang
Sun-Sik
SS
Yang
Jun
J
Lee
D000818:Animals; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000737:chemistry*; D016025:Bone Transplantation; D001842:Bone and Bones / Q000737:chemistry* / Q000378:metabolism; D002448:Cell Adhesion; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D049832:Decapodiformes; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D008297:Male; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology; D010012:Osteogenesis; D011817:Rabbits; D014961:X-Ray Diffraction
D018786:Bone Substitutes; D017886:Durapatite