Eight years of single-molecule localization microscopy.
Super-resolution imaging by single-molecule localization (localization microscopy) provides the ability to unravel the structural organization of cells and the composition of biomolecular assemblies at a spatial resolution that is well below the diffraction limit approaching virtually molecular resolution. Constant improvements in fluorescent probes, efficient and specific labeling techniques as well as refined data analysis and interpretation strategies further improved localization microscopy. Today, it allows us to interrogate how the distribution and stoichiometry of interacting proteins in subcellular compartments and molecular machines accomplishes complex interconnected cellular processes. Thus, it exhibits potential to address fundamental questions of cell and developmental biology. Here, we briefly introduce the history, basic principles, and different localization microscopy methods with special focus on direct stochastic optical reconstruction microscopy (dSTORM) and summarize key developments and examples of two- and three-dimensional localization microscopy of the last 8 years.
Department of Biotechnology and Biophysics, Biocenter, Julius Maximilian University Würzburg, Am Hubland, 97074, Würzburg, Germany, teresa.klein@uni-wuerzburg.de.
Teresa
T
Klein
Sven
S
Proppert
Markus
M
Sauer
D002477:Cells / Q000166:cytology*; D005456:Fluorescent Dyes / Q000032:analysis; D021621:Imaging, Three-Dimensional; D008856:Microscopy, Fluorescence / Q000379:methods
D005456:Fluorescent Dyes