Simple synthesis, self-assembly, and cytotoxicity of novel dimeric cholesterol derivatives.
A simple and economic methodology to synthesize three types of novel dimeric cholesterol derivatives (DCDs) was developed. Results obtained from dynamic light scattering and transmission electron microscopy show that spherical and/or angular nano-structural aggregates of DCDs are formed by self-assembly in aqueous solution. The size and morphology of DCD dispersions depend on the spatial arrangement of the substituents and polarity of the head group in the DCD structures. The cytoxicity of DCD dispersions to human keratinocytes (HaCaT) and squamous cell carcinomas (SCC25) cells was also evaluated by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The present novel DCD dispersions were not toxic to HaCaT and SCC25 cells at appropriate tested concentrations.
Department of Chemical and Materials Engineering, National Yunlin University of Science and Technology, Yunlin 64002, Taiwan. Electronic address: chouth@yuntech.edu.tw.
Tzung-Han
TH
Chou
Department of Chemical and Materials Engineering, National Yunlin University of Science and Technology, Yunlin 64002, Taiwan.
Chien-Wen
CW
Chen
Department of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan 717, Taiwan.
Chia-Hua
CH
Liang
Department of Chemical and Materials Engineering, National Yunlin University of Science and Technology, Yunlin 64002, Taiwan.
Li-Hsien
LH
Yeh
Institute of Micro/Nanotechnology, Old Dominion University, Norfolk, VA 23529, USA.
Shizhi
S
Qian
D002470:Cell Survival; D002478:Cells, Cultured; D002784:Cholesterol / Q000138:chemical synthesis* / Q000737:chemistry; D019281:Dimerization; D006801:Humans; D015603:Keratinocytes / Q000166:cytology; D008968:Molecular Conformation; D010316:Particle Size; D013499:Surface Properties
D002784:Cholesterol
Cytotoxicity; Dimeric cholesteryl derivatives; HaCaT cells; Nano-structural aggregates; Self-assembly