ESCRT machinery is required for plasma membrane repair.
Plasma membrane damage can be triggered by numerous phenomena, and efficient repair is essential for cell survival. Endocytosis, membrane patching, or extracellular budding can be used for plasma membrane repair. We found that endosomal sorting complex required for transport (ESCRT), involved previously in membrane budding and fission, plays a critical role in plasma membrane repair. ESCRT proteins were recruited within seconds to plasma membrane wounds. Quantitative analysis of wound closure kinetics coupled to mathematical modeling suggested that ESCRTs are involved in the repair of small wounds. Real-time imaging and correlative scanning electron microscopy (SEM) identified extracellular buds and shedding at the site of ESCRT recruitment. Thus, the repair of certain wounds is ensured by ESCRT-mediated extracellular shedding of wounded portions.
Institut Curie, 26 rue d'Ulm, 75248 Paris Cedex 05, France.
Ana Joaquina
AJ
Jimenez
Paolo
P
Maiuri
Julie
J
Lafaurie-Janvore
Séverine
S
Divoux
Matthieu
M
Piel
Franck
F
Perez
D001426:Bacterial Proteins / Q000494:pharmacology; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure*; D004072:Digitonin / Q000494:pharmacology; D056827:Endosomal Sorting Complexes Required for Transport / Q000235:genetics / Q000378:metabolism*; D005453:Fluorescence; D055785:Gene Knockdown Techniques; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D011419:Propidium / Q000032:analysis / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D012503:Saponins / Q000494:pharmacology; D013301:Streptolysins / Q000494:pharmacology
D001426:Bacterial Proteins; C498543:CHMP3 protein, human; C478397:CHMP4B protein, human; D002135:Calcium-Binding Proteins; D018797:Cell Cycle Proteins; D056827:Endosomal Sorting Complexes Required for Transport; C117020:PDCD6IP protein, human; D034741:RNA, Small Interfering; D012503:Saponins; D013301:Streptolysins; C485184:enhanced green fluorescent protein; C025964:streptolysin O; D049452:Green Fluorescent Proteins; D011419:Propidium; D004072:Digitonin