[Spatiotemporal expression of ADAM10 during cranial base suture development].
OBJECTIVE
To explore the expression of a disintegrin and metalloproteinase 10 (ADAM10) during mouse's development of cranial base synchondrosis.


METHODS
Histological and biochemical method including alcian blue-alizarin red staining, IHC, ICC and Western blot were applied to observe and analyze the expression of ADAM10 in mouse cranial base.


RESULTS
The spheno-occipital synchondrosis and spheno-ethmoid synchondrosis were of similar histological structure, including resting zone, proliferative zone and hypertrophic zone. ADAM10 was expressed widely in synchondrosis,especially in the hypertrophic zone. During development of cranial base, ADAM10 was highly expressed from embryo period to infantile period, while there was a huge decrease when it came to adulthood. As an excellent cell line to mimic chondrogenesis, ATDC5 expressed ADAM10 in cytoplasm, particularly around the nucleus.


CONCLUSIONS
The expression of ADAM10 during mouse's development of cranial base synchondrosis is spatiotemprally different. Supported by National Natural Science Foundation of China (10972142), National Science Foundation for Distinguished Young Scholars of China (81000451),Science Foundation for Young Scholars of Shanghai Municipal Public Health Bureau (2010y142), Foundation for Distinguished Youth Teachers of Shanghai Municipal Commission of Education [(2010)83], Key Research Project of Science and Technology Committee of Shanghai Municipality (12JC1405700) and Innovation Team Project of Shanghai Municipal Commission of Education.
Department of Oral and Craniomaxillofacial Science, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology. Shanghai 200011, China.E-mail: furunqing@hotmail.com.
Run-qing
RQ
Fu
Yu
Y
Tan
Zhi
Z
Yang
Bing
B
Fang
D051722:ADAM Proteins; D000072197:ADAM10 Protein; D053829:Amyloid Precursor Protein Secretases; D000818:Animals; D006984:Hypertrophy; D008565:Membrane Proteins; D051379:Mice; D019291:Skull Base; D013537:Sutures
D008565:Membrane Proteins; D053829:Amyloid Precursor Protein Secretases; D051722:ADAM Proteins; D000072197:ADAM10 Protein; C491234:Adam10 protein, mouse