An orthogonal DNA replication system in yeast.
An extranuclear replication system, consisting of an orthogonal DNA plasmid-DNA polymerase pair, was developed in Saccharomyces cerevisiae. Engineered error-prone DNA polymerases showed complete mutational targeting in vivo: per-base mutation rates on the plasmid were increased substantially and remained stable with no increase in genomic rates. Orthogonal replication serves as a platform for in vivo continuous evolution and as a system whose replicative properties can be manipulated independently of the host's.
Department of Biomedical Engineering, University of California-Irvine, Irvine, California, USA.
Arjun
A
Ravikumar
Department of Biomedical Engineering, University of California-Irvine, Irvine, California, USA.
Adrian
A
Arrieta
1] Department of Biomedical Engineering, University of California-Irvine, Irvine, California, USA. [2] Department of Chemistry, University of California-Irvine, Irvine, California, USA. [3] Center for Complex Biological Systems, University of California-Irvine, Irvine, California, USA.
Chang C
CC
Liu
D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D015202:Protein Engineering; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
D004259:DNA-Directed DNA Polymerase