Association between endothelial nitric oxide synthase intron 4a/b polymorphism and aortic dissection.
The genetic risk factors that contribute to the risk of developing aortic dissection (AD) have been studied. We assessed the association of endothelial nitric oxide synthase (eNOS) gene polymorphism with AD.



Patients who underwent surgery with the diagnosis of AD and survived after the operation in our center between May 2007 and June 2011 were recruited retrospectively. The eNOS intron 4a/b polymorphism was determined by polymerase chain reaction (PCR) using oligonucleotide primers (sense: 5'-AGGCCCTATGGTAGTGCCTTT-3'; antisense: 5'-TCTCTTAGTGCTGTGGTCAC-3') that flank the region of the 27 bp VNTR in intron 4.



Thirty-nine patients (88%) had type A AD, while the remainder (12%) had type B AD. The distribution of eNOS4 a/b gene polymorphism differed significantly from the control group, with higher frequencies of eNOS 4a/a and 4a/b genotypes in the AD group (x(2)=7.16, p=0.03).



In this study, the distribution of eNOS genotypes differed between the AD and control groups; however, this polymorphism was not found to be an independent factor for the development of AD.
Department of Cardiology, Siyami Ersek Cardiovascular and Thoracic Surgery Center, İstanbul, Turkey. ahmetekmekci@yahoo.com.
Ahmet
A
Ekmekçi
Department of Cardiology, Kadirli State Hospital, Osmaniye, Turkey.
Mahmut
M
Uluganyan
Department of Cardiology, Siyami Ersek Cardiovascular and Thoracic Surgery Center, İstanbul, Turkey.
Barış
B
Güngör
Department of Genetics, İstanbul University, Institute for Experimental Medical Research, İstanbul, Turkey.
Neslihan
N
Abacı
Department of Cardiology, Siyami Ersek Cardiovascular and Thoracic Surgery Center, İstanbul, Turkey.
Kazım Serhan
KS
Ozcan
Department of Cardiology, Siyami Ersek Cardiovascular and Thoracic Surgery Center, İstanbul, Turkey.
Gökhan
G
Ertaş
Department of Cardiology, Siyami Ersek Cardiovascular and Thoracic Surgery Center, İstanbul, Turkey.
Aycan
A
Zencirci
Department of Cardiovascular Surgery, Siyami Ersek Cardiovascular and Thoracic Surgery Center, İstanbul, Turkey.
Ahmet Yavuz
AY
Balcı
Department of Genetics, İstanbul University, Institute for Experimental Medical Research, İstanbul, Turkey.
Sema
S
Sırma Ekmekci
Department of Cardiology, Siyami Ersek Cardiovascular and Thoracic Surgery Center, İstanbul, Turkey.
Nurten
N
Sayar
Department of Genetics, İstanbul University, Institute for Experimental Medical Research, İstanbul, Turkey.
Duran
D
Ustek
Department of Cardiology, Siyami Ersek Cardiovascular and Thoracic Surgery Center, İstanbul, Turkey.
Mehmet
M
Eren
D000368:Aged; D000784:Aortic Dissection / Q000235:genetics*; D017545:Aortic Aneurysm, Thoracic / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007438:Introns / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D012189:Retrospective Studies
C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III