A remodeled protein arginine methyltransferase 1 (PRMT1) generates symmetric dimethylarginine.
Protein arginine methylation is emerging as a significant post-translational modification involved in various cell processes and human diseases. As the major arginine methylation enzyme, protein arginine methyltransferase 1 (PRMT1) strictly generates monomethylarginine and asymmetric dimethylarginine (ADMA), but not symmetric dimethylarginine (SDMA). The two types of dimethylarginines can lead to distinct biological outputs, as highlighted in the PRMT-dependent epigenetic control of transcription. However, it remains unclear how PRMT1 product specificity is regulated. We discovered that a single amino acid mutation (Met-48 to Phe) in the PRMT1 active site enables PRMT1 to generate both ADMA and SDMA. Due to the limited amount of SDMA formed, we carried out quantum mechanical calculations to determine the free energies of activation of ADMA and SDMA synthesis. Our results indicate that the higher energy barrier of SDMA formation (ΔΔG(‡) = 3.2 kcal/mol as compared with ADMA) may explain the small amount of SDMA generated by M48F-PRMT1. Our study reveals unique energetic challenges for SDMA-forming methyltransferases and highlights the exquisite control of product formation by active site residues in the PRMTs.
From the Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322.
Shanying
S
Gui
Symon
S
Gathiaka
Jun
J
Li
Jun
J
Qu
Orlando
O
Acevedo
Joan M
JM
Hevel
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry; D020134:Catalytic Domain; D006801:Humans; D056004:Molecular Dynamics Simulation; D009154:Mutation; D011484:Protein-Arginine N-Methyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D013379:Substrate Specificity; D013816:Thermodynamics
C487735:dimethylarginine; D001120:Arginine; C541817:PRMT1 protein, rat; D011484:Protein-Arginine N-Methyltransferases
Computer Modeling; Dimethylarginine; Enzyme Mechanisms; Histone Methylation; Product Specificity; Protein Arginine Methyltransferase; Protein Methylation; S-adenosylmethionine (SAM); Site-directed Mutagenesis