Temperature impacts on anaerobic biotransformation of LNAPL and concurrent shifts in microbial community structure.
Thermally-enhanced bioremediation is a promising treatment approach for petroleum contamination; however, studies examining temperature effects on anaerobic biodegradation in zones containing light non-aqueous phase liquids (LNAPLs) are lacking. Herein, laboratory microcosm studies were conducted for a former refinery to evaluate LNAPL transformation, sulfate reduction, and methane generation over a one-year period for temperatures ranging from 4 to 40 °C, and microbial community shifts were characterized. Temperatures of 22 and 30 °C significantly increased total biogas generation compared to lower (4 and 9 °C) and higher temperatures (35 and 40 °C; p < 0.1). Additionally, at 22 and 30 °C methane generation commenced ~6 months earlier than for 35 and 40 °C. Statistically significant biodegradation of benzene, toluene and xylenes was observed at elevated temperatures but not at lower temperatures (p < 0.1). Additionally, a novel differential chromatogram approach was developed to overcome challenges associated with resolving losses in complex mixtures of hydrocarbons, and application of this method revealed greater losses of hydrocarbons at 22 and 30 °C as compared to lower and higher temperatures. Finally, molecular biology assays revealed that the composition and activity of microbial communities shifted in a temperature-dependent manner. Collectively, results demonstrated that anaerobic biodegradation processes can be enhanced by increasing the temperature of LNAPL-containing soils, but biodegradation does not simply increase as temperature increases likely due to a lack of microorganisms that thrive at temperatures well above the historical high temperatures for a site. Rather, optimal degradation is achieved by holding soils at the high end of, or slightly higher than, their natural range.
Department of Civil and Environmental Engineering, Colorado State University, 1301 Campus Delivery, Fort Collins, CO, 80523, USA.
Natalie R
NR
Zeman
Maria
M
Irianni Renno
Mitchell R
MR
Olson
L Paige
LP
Wilson
Thomas C
TC
Sale
Susan K
SK
De Long
D000693:Anaerobiosis; D001105:Archaea / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D056804:Biofuels; D001711:Biotransformation; D004785:Environmental Pollutants / Q000378:metabolism*; D006838:Hydrocarbons / Q000378:metabolism; D008697:Methane / Q000096:biosynthesis; D059013:Microbial Consortia; D010084:Oxidation-Reduction; D013431:Sulfates / Q000378:metabolism; D013696:Temperature
D056804:Biofuels; D004785:Environmental Pollutants; D006838:Hydrocarbons; D013431:Sulfates; D008697:Methane