Multiple antibody targets on herpes B glycoproteins B and D identified by screening sera of infected rhesus macaques with peptide microarrays.
Herpes B virus (or Herpesvirus simiae or Macacine herpesvirus 1) is endemic in many populations of macaques, both in the wild and in captivity. The virus elicits only mild clinical symptoms (if any) in monkeys, but can be transmitted by various routes, most commonly via bites, to humans where it causes viral encephalitis with a high mortality rate. Hence, herpes B constitutes a considerable occupational hazard for animal caretakers, veterinarians and laboratory personnel. Efforts are therefore being made to reduce the risk of zoonotic infection and to improve prognosis after accidental exposure. Among the measures envisaged are serological surveillance of monkey colonies and specific diagnosis of herpes B zoonosis against a background of antibodies recognizing the closely related human herpes simplex virus (HSV). 422 pentadecapeptides covering, in an overlapping fashion, the entire amino acid sequences of herpes B proteins gB and gD were synthesized and immobilized on glass slides. Antibodies present in monkey sera that bind to subsets of the peptide collection were detected by microserological techniques. With 42 different rhesus macaque sera, 114 individual responses to 18 different antibody target regions (ATRs) were recorded, 17 of which had not been described earlier. This finding may pave the way for a peptide-based, herpes B specific serological diagnostic test.
Unit of Infection Models, German Primate Center, Göttingen, Germany.
Sven-Kevin
SK
Hotop
Department of Virology, University Medical Center, Georg-August University Göttingen, Göttingen, Germany.
Ahmed
A
Abd El Wahed
Department of Chemical Biology, Helmholtz Centre for Infection Research, Braunschweig, Germany.
Ulrike
U
Beutling
Unit of Infection Models, German Primate Center, Göttingen, Germany.
Dieter
D
Jentsch
Unit of Infection Models, German Primate Center, Göttingen, Germany.
Dirk
D
Motzkus
Department of Chemical Biology, Helmholtz Centre for Infection Research, Braunschweig, Germany.
Ronald
R
Frank
Department of Virology, University Medical Center, Georg-August University Göttingen, Göttingen, Germany.
Gerhard
G
Hunsmann
Unit of Infection Models, German Primate Center, Göttingen, Germany.
Christiane
C
Stahl-Hennig
Courant Research Centre Geobiology, Göttingen, Germany.
Hans-Joachim
HJ
Fritz
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000276:immunology* / Q000821:virology; D006568:Herpesvirus 1, Cercopithecine / Q000235:genetics / Q000276:immunology*; D054884:Host-Pathogen Interactions / Q000276:immunology; D006801:Humans; D008253:Macaca mulatta / Q000097:blood / Q000276:immunology* / Q000821:virology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D040081:Protein Array Analysis / Q000379:methods; D017434:Protein Structure, Tertiary; D012680:Sensitivity and Specificity; D017386:Sequence Homology, Amino Acid; D018139:Simplexvirus / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D015047:Zoonoses / Q000175:diagnosis / Q000276:immunology / Q000821:virology
D000914:Antibodies, Viral; D000939:Epitopes; D010455:Peptides; D014759:Viral Envelope Proteins