Application of solidified floating organic drop microextraction method for biomonitoring of chlorpyrifos and its oxon metabolite in urine samples.
A simple, efficient and green analytical procedure for monitoring sub ppb amounts of chlorpyrifos (CP) and chlorpyrifos-oxon (CPO) in urine samples was reported. The methodology is based on the solidified floating organic drop microextraction of the analytes with a free microdrop of 2-dodecanol. The parameters those can affect the microextraction efficiency, such as solvent type, extraction solvent volume, extraction time and temperature, salt effect, pH and stirring rate on extraction were optimized. The analytes were extracted from the urine samples by using 10μL of 2-dodecanol for 40min at 70°C and then, the extracts were injected to GC-MS column by applying 100kPa injection pressure. The regression coefficients relating to linearity were at least 0.99. The accuracy of the developed method was tested upon recovery studies for CP and CPO calculated as 100±7% and 110±9% (at 0.1ngmL(-1) level), respectively. LOD value for CP was found 4.8ngL(-1) and for CPO it was found 3.8ngL(-1). This method can easily be adopted by clinical laboratories for the contribution to policies aiming to reduce exposure of pesticides.
Ege University, Science Faculty, Department of Chemistry, 35100 Bornova, Izmir, Turkey. Electronic address: fusun.okcu@ege.edu.tr.
Füsun Okçu
FO
Pelit
Ege University, Faculty of Pharmacy, Pharmaceutical Sciences Research Laboratory (FABAL), 35100 Bornova, Izmir, Turkey.
Çiğdem
Ç
Yengin
D004390:Chlorpyrifos / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D059627:Liquid Phase Microextraction / Q000379:methods*; D010575:Pesticides / Q000032:analysis / Q000302:isolation & purification / Q000652:urine*; D015203:Reproducibility of Results
D010575:Pesticides; C009618:O,O-diethyl O-3,5,6-trichloro-2-pyridyl phosphate; D004390:Chlorpyrifos
Chlorpyrifos; Chlorpyrifos oxon; Gas chromatography; Pesticide; Solidified floating organic drop microextraction; Urine