[Effect of Siwu decoction and its combined administration on hepatic P450 enzymatic activity and mRNA expression in rats].
To study the effect of Siwu decoction (SWD) compound and its combined administration on hepatic P450 enzymatic activity and mRNA expression in rats. Rats were orally administered with SWD and water decoction combined with other medicines for two weeks, and then sacrificed. Their livers were perfused with normal saline to prepare liver micrisomes. Mixed probe and liver microsome in vitro incubation method were adopted to detect the effect of SWD on hepatic cytochrome P450. The real-time quantitative polymerase chain reaction (Q-PCR) was used to detect the effect of SWD on the expression of hepatic cytochrome P450. Compared with the control group, the SWD compound group showed higher CYP1A2 enzymatic activity (P < 0.05); Rehmanniae-paeoniae, angelicae-paeoniae, angelicae-rhizome, paeoniae-rhizome groups had lower CYP1A2 and CYP2C19 enzymatic activities (P < 0.05); And the compound group, the single component group and the combination group showed lower CYP2B6 enzymatic activities (P < 0.05). The compound could up-regulated the mRNA expression of CYP2B1 (P < 0.05); And the four single components could down-regulated the mRNA expression of CYP2B1 (P < 0.05). SWD compound had the effect in inducing CYP1A2 enzymatic activity. The rehmanniae-paeoniae group and the angelicae-paeoniae group had identical enzymatic activity with the control group, but significant down-regulation in CYP1A2 enzymatic activity after being combined with paeoniae. The compound and its combined administration showed the inhibitory effect on CYP2B6 enzymatic activity, particularly being combined with angelicae. The compound showed identical effect with the four single components in terms of CYP1A2 mRNA expression and enzymatic activity.
School of Pharmacy, Guangxi Medical University, Nanning 530021, China. liangmiao_0423@126.com
Miao
M
Liang
Research Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
Zeng-Chun
ZC
Ma
Research Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
Jian-Feng
JF
Yi
Research Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
Yu-Guang
YG
Wang
Research Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
Hong-Ling
HL
Tan
Research Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
Cheng-Rong
CR
Xiao
Research Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
Qian-De
QD
Liang
Research Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
Xiang-Lin
XL
Tang
Research Institute of Toxicology and Pharmacology, Academy of Military Medical Science, Beijing 100850, China.
Hua
H
Li
Research Institute of Toxicology and Pharmacology, Academy of Military Medical Science, Beijing 100850, China.
Guo-Lin
GL
Shen
Research Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
Yue
Y
Gao
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System