Post-transcriptional regulation of ITGB6 protein levels in damaged skeletal muscle.
We have identified integrin beta 6 (Itgb6) as a transcript highly enriched in skeletal muscle. This finding is unexpected because Itgb6 is typically associated with epithelial expression domains in normal tissue. Further we find that ITGB6 protein expression in muscle is post-transcriptionally regulated. Uninjured muscle expresses Itgb6 RNA but no ITGB6 protein is detectable. Muscle injury induces ITGB6 protein accumulation rapidly post-injury in myofibers adjacent to the site of injury. As regeneration of the injured muscle tissue progresses ITGB6 protein is found in newly formed fibers up to at least 15 days post-injury.
Target and Pathway Validation, Molecular Discovery Research, GlaxoSmithKline, Research Triangle Park, NC, USA.
Melissa
M
Ducceschi
Lisa G
LG
Clifton
Stephen A
SA
Stimpson
Andrew N
AN
Billin
D000818:Animals; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D039641:Integrin beta Chains / Q000235:genetics* / Q000378:metabolism*; D008297:Male; D051379:Mice; D018482:Muscle, Skeletal / Q000293:injuries / Q000378:metabolism* / Q000473:pathology; D009135:Muscular Diseases / Q000235:genetics / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D016212:Transforming Growth Factor beta / Q000378:metabolism
D039641:Integrin beta Chains; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; C072170:integrin beta6