Salicylic acid induces vanillin synthesis through the phospholipid signaling pathway in Capsicum chinense cell cultures.
Signal transduction via phospholipids is mediated by phospholipases such as phospholipase C (PLC) and D (PLD), which catalyze hydrolysis of plasma membrane structural phospholipids. Phospholipid signaling is also involved in plant responses to phytohormones such as salicylic acid (SA). The relationships between phospholipid signaling, SA, and secondary metabolism are not fully understood. Using a Capsicum chinense cell suspension as a model, we evaluated whether phospholipid signaling modulates SA-induced vanillin production through the activation of phenylalanine ammonia lyase (PAL), a key enzyme in the biosynthetic pathway. Salicylic acid was found to elicit PAL activity and consequently vanillin production, which was diminished or reversed upon exposure to the phosphoinositide-phospholipase C (PI-PLC) signaling inhibitors neomycin and U73122. Exposure to the phosphatidic acid inhibitor 1-butanol altered PLD activity and prevented SA-induced vanillin production. Our results suggest that PLC and PLD-generated secondary messengers may be modulating SA-induced vanillin production through the activation of key biosynthetic pathway enzymes.
Beatriz A
BA
Rodas-Junco
Yahaira
Y
Cab-Guillén
J Armando
JA
Muñoz-Sánchez
Felipe
F
Vázquez-Flota
Miriam
M
Monforte-González
S M Teresa
SM
Hernández-Sotomayor
D001547:Benzaldehydes / Q000378:metabolism*; D002212:Capsicum / Q000187:drug effects / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D020156:Salicylic Acid / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects
D001547:Benzaldehydes; D010743:Phospholipids; C100058:vanillin; D020156:Salicylic Acid