Ribosomal and mitochondrial peroxidase isoenzymes of the lentil (Lens culinaris) root.
Ribosome- and mitochondria-rich preparations were obtained from lentil roots and their peroxidase isoenzymes examined by starch gel electrophoresis. Seven isoenzymes were shown to be associated with both the ribosomal and mitochondrial fractions.The apparent similarity between ribosomal and mitochondrial isoenzyme patterns, together with the observation that a considerable amount of peroxidase activity dissociated from the ribosomes during sedimentation of the ribosomal preparation into a sucrose gradient, suggested that at least some of these isoperoxidases were attached to membranes.
Woodstock Agricultural Research Centre, Shell Research Limited, Sittingbourne, Kent, England.
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E
Darimont
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R
Baxter