Detecting epigenetic effects of transposable elements in plants.
Transposable elements (TE) represent a major fraction of eukaryotic genomes and play many roles in plant epigenetics. In this chapter, we describe the use of Sequence-Specific Amplified Polymorphism (SSAP) as a reliable Transposon Display technique applicable for use in many plant species. We also discuss the interpretation of SSAP data and associated risks. This technique has potential to allow rapid screening of plant populations, especially in nonmodel or wild species.
Laboratory of Evolutionary Botany, Biology Institute, University of Neuchâtel, Neuchâtel, Switzerland.
Christian
C
Parisod
Armel
A
Salmon
Malika
M
Ainouche
Marie-Angèle
MA
Grandbastien
D019175:DNA Methylation; D004251:DNA Transposable Elements / Q000235:genetics*; D044127:Epigenesis, Genetic; D010944:Plants / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015183:Restriction Mapping
D004251:DNA Transposable Elements