NMDA and AMPA receptors in the anterior cingulate cortex mediates visceral pain in visceral hypersensitivity rats.
Several studies have shown that N-methyl-D-aspartate (NMDA)-receptor activation in anterior cingulate cortex (ACC) neurons plays critical roles in modulating visceral pain responses in visceral hypersensitivity (VH) rats. However, there are few reports about the expressions of NMDA and α-amino-3-hydroxy-5-methyl-4-isox-azolepropionic-acid (AMPA) receptor subtypes in ACC of VH model rats at different time points. The current study was undertaken to investigate NR2A, NR2B and GluR2 expressions in ACC of VH rats that were induced by administration with 5% mustard oil. Our results indicated that NR2B, but not NR2A, was highly expressed in VH model group on day 15, 22, and 36 compared with normal group (p < 0.05). GluR2 expression was also higher in VH model group on day 15, 22, and 36 than that of normal group (p < 0.05). These findings suggested increased expression of NR2B and GluR2 might be key mechanisms for long-term synaptic plastic changes in VH rats.
Department of Paediatrics, Changhai Hospital of Second Military Medical University, Shanghai 200433, China.
Lin
L
Zhou
Department of Paediatrics, Changhai Hospital of Second Military Medical University, Shanghai 200433, China.
Junjing
J
Huang
Department of Gastroenterology, Changhai Hospital of Second Military Medical University, Shanghai 200433, China.
Jun
J
Gao
Department of Gastroenterology, Changhai Hospital of Second Military Medical University, Shanghai 200433, China.
Guanpo
G
Zhang
Department of Paediatrics, Changhai Hospital of Second Military Medical University, Shanghai 200433, China. Electronic address: jinjinj2000@163.com.
Jinjin
J
Jiang
D000285:Administration, Rectal; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D004195:Disease Models, Animal; D006179:Gyrus Cinguli / Q000378:metabolism; D006801:Humans; D006967:Hypersensitivity / Q000378:metabolism*; D009149:Mustard Plant; D009473:Neuronal Plasticity; D010938:Plant Oils / Q000008:administration & dosage; D051381:Rats; D017207:Rats, Sprague-Dawley; D018091:Receptors, AMPA / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D015398:Signal Transduction; D059265:Visceral Pain / Q000378:metabolism*
C120997:NR2A NMDA receptor; C121001:NR2B NMDA receptor; D010938:Plant Oils; D018091:Receptors, AMPA; D016194:Receptors, N-Methyl-D-Aspartate; C104722:glutamate receptor ionotropic, AMPA 2; C027793:mustard oil
Anterior cingulate cortex; N-methyl-d-aspartate; Visceral hypersensitivity; α-Amino-3-hydroxy-5-methyl-4-isox-azolepropionic-acid