Development of barley (Hordeum vulgare L.) lines with altered starch granule size distribution.
Microscope analysis of starches prepared from 139 barley genotypes identified a Japanese genotype, Kinai Kyoshinkai-2 (KK-2), with altered starch granule size distribution. Compared to normal barley starch, KK-2 produced consistently higher volumes of starch granules with 5-15 μm diameter and reduced volumes of starch granules with >15 μm diameter when grown in different environments. A cross between KK-2 and normal starch cultivar CDC Kendall was made and led to the production of 154 F5 lines with alterations to the normal 7:3:1 distribution for A-:B-:C-type starch granule volumes. Three F5 lines showed unimodal starch granule size distribution due to apparent lack of very small (<5.0 μm diameter) C-type starch granules, but the phenotype was accompanied by reduced grain weight and total starch concentration. Five F5 lines produced a significantly larger population of large (>15 μm diameter) A-type starch granules as compared to normal starch and showed on average a 10:4:1 distribution for A-:B-:C-type starch granule volumes. The unusual starch phenotypes displayed by the F5 lines confirm starch granule size distribution in barley can be genetically altered.
Department of Plant Sciences, College of Agriculture and Bioresources, University of Saskatchewan , 51 Campus Drive, Saskatoon, Saskatchewan S7N5A8, Canada.
Sarita
S
Jaiswal
Monica
M
Båga
Geetika
G
Ahuja
Brian G
BG
Rossnagel
Ravindra N
RN
Chibbar
D005838:Genotype; D001467:Hordeum / Q000166:cytology* / Q000235:genetics*; D010641:Phenotype; D012639:Seeds / Q000235:genetics; D013213:Starch / Q000032:analysis / Q000737:chemistry* / Q000235:genetics*
D013213:Starch