High-density lipoprotein subfractions and influence of endothelial lipase in a healthy Turkish population: a study in a land of low high-density lipoprotein cholesterol.
OBJECTIVE
Low concentration of high-density lipoprotein (HDL) is prevalent in Turkey. Endothelial lipase (EL) regulates lipoprotein metabolism. Small, lipid-poor HDL particles represent more-efficient cholesterol acceptors than their large, lipid-rich counterparts. The aim of this study was to investigate HDL subfractions and the effect of EL on HDL concentrations in healthy Turkish population.


METHODS
102 healthy subjects were included in the study (mean age 33.6 ± 10.3 years, 42 female). HDL subfractions were assayed by single precipitation method and EL concentrations were measured by competitive enzyme immunoassay.


RESULTS
Mean HDL concentrations were 1.45 ± 0.37 mmol/L in women, 1.10 ± 0.30 mmol/L in men. Small HDL subfraction levels did not differ statistically between < 1 mmol/L and ≥ 1.6 mmol/L total HDL groups. Small HDL was not correlated with EL, low density lipoprotein cholesterol (LDL), triglyceride (TG) and age but positively correlated with total cholesterol and HDL (r = 0.2, p = 0.017; r = 0.2, p = 0.028, respectively). Large HDL was not correlated with age, EL and total cholesterol, and negatively correlated with HDL, LDL, TG (r = - 0.7, p < 0.001; r = - 0.2, p = 0.045; r = - 0.3, p < 0.001, respectively). If subjects were divided into two groups as HDL< 1 mmol/L and HDL > 1.6 mmol/L, mean EL concentrations were 475.83 ± 521.77 nmol/L and 529.71 ± 276.92 nmol/L, respectively (p = 0.086).


CONCLUSIONS
There were no differences between small HDL concentrations in the HDL low and high groups. Our data did not support EL to be the reason for low HDL in a healthy Turkish population. Our results in a healthy population may serve as a reference for clinical studies on HDL subfractions.
Department of Cardiology, Faculty of Medicine, Sakarya University , Sakarya.
Harun
H
Kilic
Enver
E
Atalar
Incilay
I
Lay
Nuray
N
Yazihan
Fatih
F
Buyukcam
Ugur
U
Saygisunar
Murat
M
Aksoy
Huseyin
H
Gunduz
Ramazan
R
Akdemir
D000328:Adult; D008078:Cholesterol, LDL / Q000097:blood*; D005260:Female; D006801:Humans; D008049:Lipase / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood; D008297:Male; D014280:Triglycerides / Q000097:blood; D014421:Turkey
D008078:Cholesterol, LDL; D008075:Lipoproteins, HDL; D014280:Triglycerides; C410382:LIPG protein, human; D008049:Lipase
HDL subfractions; endothelial lipase; lipoprotein metabolism