Comprehensive genome-wide analysis reveals different classes of enigmatic old yellow enzyme in fungi.
In this study, we systematically identify Old Yellow Enzymes (OYEs) from a diverse range of economically important fungi representing different ecology and lifestyle. Using active site residues and sequence alignments, we present a classification for these proteins into three distinct classes including a novel class (Class III) and assign names to sequences. Our in-depth phylogenetic analysis suggests a complex history of lineage-specific expansion and contraction for the OYE gene family in fungi. Comparative analyses reveal remarkable diversity in the number and classes of OYE among fungi. Quantitative real-time PCR (qRT-PCR) of Ascochyta rabiei OYEs indicates differential expression of OYE genes during oxidative stress and plant infection. This study shows relationship of OYE with fungal ecology and lifestyle, and provides a foundation for future functional analysis and characterization of OYE gene family.
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi-110067, India.
Shadab
S
Nizam
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi-110067, India.
Sandhya
S
Verma
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi-110067, India.
Nilam Nayan
NN
Borah
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi-110067, India.
Rajesh Kumar
RK
Gazara
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi-110067, India.
Praveen Kumar
PK
Verma
D000595:Amino Acid Sequence; D001203:Ascomycota / Q000201:enzymology* / Q000235:genetics*; D019070:Cell Lineage / Q000235:genetics; D019143:Evolution, Molecular; D020869:Gene Expression Profiling; D014644:Genetic Variation; D009252:NADPH Dehydrogenase / Q000096:biosynthesis / Q000145:classification* / Q000235:genetics*; D018384:Oxidative Stress; D010802:Phylogeny; D016415:Sequence Alignment
D009252:NADPH Dehydrogenase