Purification and characterization of plantaricin Y, a novel bacteriocin produced by Lactobacillus plantarum 510.
Lactobacillus plantarum 510, previously isolated from a koshu vineyard in Japan, was found to produce a bacteriocin-like inhibitory substance which was purified and characterized. Mass spectrometry analysis showed that the mass of this bacteriocin is 4,296.65 Da. A partial sequence, NH2- SSSLLNTAWRKFG, was obtained by N-terminal amino acid sequence analysis. A BLAST search revealed that this is a unique sequence; this peptide is thus a novel bacteriocin produced by Lactobacillus plantarum 510 and was termed plantaricin Y. Plantaricin Y shows strong inhibitory activity against Listeria monocytogenes BCRC 14845, but no activity against other pathogens tested. Bacteriocin activity decreased slightly after autoclaving (121 °C for 15 min), but was completely inactivated by protease K. Furthermore, trypsin-digested bacteriocin product fragments retained activity against L. monocytogenes BCRC 14845 and exhibited a different inhibitory spectrum.
Department of Biotechnology, Ming Chuan University, No. 5 De-Ming Rd., Gui-Shan Township, Taoyuan, 333, Taiwan, yisheng@mail.mcu.edu.tw.
Yi-sheng
YS
Chen
Yan-chong
YC
Wang
Yiou-shing
YS
Chow
Fujitoshi
F
Yanagida
Chen-chung
CC
Liao
Chi-ming
CM
Chiu
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001430:Bacteriocins / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D019286:Endopeptidase K / Q000378:metabolism; D006358:Hot Temperature; D007564:Japan; D048191:Lactiplantibacillus plantarum / Q000737:chemistry* / Q000235:genetics; D008089:Listeria monocytogenes / Q000187:drug effects / Q000378:metabolism; D013058:Mass Spectrometry; D008970:Molecular Weight
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D019286:Endopeptidase K