The promoter of the Arabidopsis PIN6 auxin transporter enabled strong expression in the vasculature of roots, leaves, floral stems and reproductive organs.
Cellular auxin homeostasis controls many aspects of plant growth, organogenesis and development. The existence of intracellular auxin transport mediated by endoplasmic reticulum (ER)-localized PIN5, PIN6 and PIN8 proteins is a relatively recent discovery shaping a new era in understanding auxin-mediated growth processes. Here we summarize the importance of PIN6 in mediating intracellular auxin transport during root formation, leaf vein patterning and nectary production. While, it was previously shown that PIN6 was strongly expressed in rosette leaf cell types important in vein formation, here we demonstrate by use a PIN6 promoter-reporter fusion, that PIN6 is also preferentially expressed in the vasculature of the primary root, cotyledons, cauline leaves, floral stem, sepals and the main transmitting tract of the reproductive silique. The strong, vein- specific reporter gene expression patterns enabled by the PIN6 promoter emphasizes that transcriptional control is likely to be a major regulator of PIN6 protein levels, during vasculature formation, and supports the need for ER-localized PIN proteins in selecting specialized cells for vascular function in land plants.
Australian Research Council Centre of Excellence in Plant Energy Biology; College of Medicine, Biology and Environment; Research School of Biology; The Australian National University; Canberra, ACT Australia.
Nazia
N
Nisar
Australian Research Council Centre of Excellence in Plant Energy Biology; College of Medicine, Biology and Environment; Research School of Biology; The Australian National University; Canberra, ACT Australia.
Abby J
AJ
Cuttriss
Australian Research Council Centre of Excellence in Plant Energy Biology; College of Medicine, Biology and Environment; Research School of Biology; The Australian National University; Canberra, ACT Australia.
Barry J
BJ
Pogson
Australian Research Council Centre of Excellence in Plant Energy Biology; College of Medicine, Biology and Environment; Research School of Biology; The Australian National University; Canberra, ACT Australia.
Christopher I
CI
Cazzonelli
D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D007210:Indoleacetic Acids / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D063245:Plant Development; D057048:Plant Nectar / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development; D058526:Plant Vascular Bundle / Q000254:growth & development / Q000378:metabolism*; D011401:Promoter Regions, Genetic
D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D026901:Membrane Transport Proteins; C581623:PIN6 protein, Arabidopsis; D057048:Plant Nectar
PIN6; auxin; development; hormone; transport; vasculature