The outer membrane protein P2 (OmpP2) of Haemophilus parasuis induces proinflammatory cytokine mRNA expression in porcine alveolar macrophages.
Porins, expressed by Gram-negative bacteria, have several biological effects on host tissues or cells. The outer membrane protein P2 (OmpP2), a member of the porin family, has been identified as a multifunctional protein involved in the pathogenicity of Haemophilus parasuis. In the present study, it was shown that OmpP2 (0.5-10 μg/mL) from H. parasuis Nagasaki strain up-regulated mRNA expression of interleukin (IL)-1α, IL-1β, IL-6 and IL-8 in porcine alveolar macrophages (PAM, 3D4/31) in vitro, in a dose-dependent manner. Moreover, OmpP2 porin induced a more prolonged cytokine response in PAM than that of the lipooligosaccharide (LOS) from this microorganism. The data demonstrate that H. parasuis OmpP2 can stimulate proinflammatory cytokine mRNA expression, suggesting that this particular porin might play an important role in the pathogenesis of disease caused by H. parasuis.
Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, People's Republic of China; Key Laboratory of Applied Marine Biotechnology, School of Marine Science, Ningbo University, Ningbo 315211, People's Republic of China.
Suming
S
Zhou
Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, People's Republic of China.
Xianhui
X
He
Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, People's Republic of China.
Chenggang
C
Xu
Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, People's Republic of China; College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, People's Republic of China.
Bing
B
Zhang
Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, People's Republic of China.
Saixiang
S
Feng
Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, People's Republic of China.
Yi
Y
Zou
Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, People's Republic of China.
Jingyi
J
Li
Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, People's Republic of China. Electronic address: mliao@scau.edu.cn.
Ming
M
Liao
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D044137:Haemophilus parasuis / Q000235:genetics* / Q000378:metabolism; D007378:Interleukins / Q000235:genetics / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000276:immunology*; D018272:Porins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013552:Swine
D001425:Bacterial Outer Membrane Proteins; D007378:Interleukins; C063931:Omp2 protein, bacteria; D018272:Porins; D012333:RNA, Messenger
Alveolar macrophage; Haemophilus parasuis; OmpP2 porin; Proinflammatory cytokines