Comparison of serum amyloid A and C-reactive protein as diagnostic markers of systemic inflammation in dogs.
The diagnostic performance of canine serum amyloid A (SAA) was compared with that of C-reactive protein (CRP) in the detection of systemic inflammation in dogs. Sera from 500 dogs were retrospectively included in the study. C-reactive protein and SAA were measured using validated automated assays. The overlap performance, clinical decision limits, overall diagnostic performance, correlations, and agreement in the clinical classification between these 2 diagnostic markers were compared. Significantly higher concentrations of both proteins were detected in dogs with systemic inflammation (SAA range: 48.75 to > 2700 mg/L; CRP range: 0.4 to 907.4 mg/L) compared to dogs without systemic inflammation (SAA range: 1.06 to 56.4 mg/L; CRP range: 0.07 to 24.7 mg/L). Both proteins were shown to be sensitive and specific markers of systemic inflammation in dogs. Significant correlations and excellent diagnostic agreement were observed between the 2 markers. However, SAA showed a wider range of concentrations and a significantly superior overall diagnostic performance compared with CRP.
Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Groennegaardsvej 3 ground floor, Frederiksberg, Denmark (Christensen, Langhorn, Andreasen, Jacobsen, Persson, Kjelgaard-Hansen); Department of Companion Animal Clinical Studies, University of Pretoria, Private Bag X04, Onderstepoort, 0110, Pretoria, South Africa (Goddard); Department of Companion Animal Sciences, Norwegian School of Veterinary Science, P.O. Box 8146 Dep. N-0033 Oslo, Norway (Moldal); Department of Animal Medicine and Surgery, the Veterinary Faculty, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain (Tvarijonaviciute); and Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 108, 3584 CM Utrecht, Netherlands (Kirpensteijn).
Michelle B
MB
Christensen
Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Groennegaardsvej 3 ground floor, Frederiksberg, Denmark (Christensen, Langhorn, Andreasen, Jacobsen, Persson, Kjelgaard-Hansen); Department of Companion Animal Clinical Studies, University of Pretoria, Private Bag X04, Onderstepoort, 0110, Pretoria, South Africa (Goddard); Department of Companion Animal Sciences, Norwegian School of Veterinary Science, P.O. Box 8146 Dep. N-0033 Oslo, Norway (Moldal); Department of Animal Medicine and Surgery, the Veterinary Faculty, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain (Tvarijonaviciute); and Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 108, 3584 CM Utrecht, Netherlands (Kirpensteijn).
Rebecca
R
Langhorn
Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Groennegaardsvej 3 ground floor, Frederiksberg, Denmark (Christensen, Langhorn, Andreasen, Jacobsen, Persson, Kjelgaard-Hansen); Department of Companion Animal Clinical Studies, University of Pretoria, Private Bag X04, Onderstepoort, 0110, Pretoria, South Africa (Goddard); Department of Companion Animal Sciences, Norwegian School of Veterinary Science, P.O. Box 8146 Dep. N-0033 Oslo, Norway (Moldal); Department of Animal Medicine and Surgery, the Veterinary Faculty, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain (Tvarijonaviciute); and Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 108, 3584 CM Utrecht, Netherlands (Kirpensteijn).
Amelia
A
Goddard
Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Groennegaardsvej 3 ground floor, Frederiksberg, Denmark (Christensen, Langhorn, Andreasen, Jacobsen, Persson, Kjelgaard-Hansen); Department of Companion Animal Clinical Studies, University of Pretoria, Private Bag X04, Onderstepoort, 0110, Pretoria, South Africa (Goddard); Department of Companion Animal Sciences, Norwegian School of Veterinary Science, P.O. Box 8146 Dep. N-0033 Oslo, Norway (Moldal); Department of Animal Medicine and Surgery, the Veterinary Faculty, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain (Tvarijonaviciute); and Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 108, 3584 CM Utrecht, Netherlands (Kirpensteijn).
Eva B
EB
Andreasen
Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Groennegaardsvej 3 ground floor, Frederiksberg, Denmark (Christensen, Langhorn, Andreasen, Jacobsen, Persson, Kjelgaard-Hansen); Department of Companion Animal Clinical Studies, University of Pretoria, Private Bag X04, Onderstepoort, 0110, Pretoria, South Africa (Goddard); Department of Companion Animal Sciences, Norwegian School of Veterinary Science, P.O. Box 8146 Dep. N-0033 Oslo, Norway (Moldal); Department of Animal Medicine and Surgery, the Veterinary Faculty, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain (Tvarijonaviciute); and Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 108, 3584 CM Utrecht, Netherlands (Kirpensteijn).
Elena
E
Moldal
Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Groennegaardsvej 3 ground floor, Frederiksberg, Denmark (Christensen, Langhorn, Andreasen, Jacobsen, Persson, Kjelgaard-Hansen); Department of Companion Animal Clinical Studies, University of Pretoria, Private Bag X04, Onderstepoort, 0110, Pretoria, South Africa (Goddard); Department of Companion Animal Sciences, Norwegian School of Veterinary Science, P.O. Box 8146 Dep. N-0033 Oslo, Norway (Moldal); Department of Animal Medicine and Surgery, the Veterinary Faculty, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain (Tvarijonaviciute); and Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 108, 3584 CM Utrecht, Netherlands (Kirpensteijn).
Asta
A
Tvarijonaviciute
Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Groennegaardsvej 3 ground floor, Frederiksberg, Denmark (Christensen, Langhorn, Andreasen, Jacobsen, Persson, Kjelgaard-Hansen); Department of Companion Animal Clinical Studies, University of Pretoria, Private Bag X04, Onderstepoort, 0110, Pretoria, South Africa (Goddard); Department of Companion Animal Sciences, Norwegian School of Veterinary Science, P.O. Box 8146 Dep. N-0033 Oslo, Norway (Moldal); Department of Animal Medicine and Surgery, the Veterinary Faculty, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain (Tvarijonaviciute); and Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 108, 3584 CM Utrecht, Netherlands (Kirpensteijn).
Jolle
J
Kirpensteijn
Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Groennegaardsvej 3 ground floor, Frederiksberg, Denmark (Christensen, Langhorn, Andreasen, Jacobsen, Persson, Kjelgaard-Hansen); Department of Companion Animal Clinical Studies, University of Pretoria, Private Bag X04, Onderstepoort, 0110, Pretoria, South Africa (Goddard); Department of Companion Animal Sciences, Norwegian School of Veterinary Science, P.O. Box 8146 Dep. N-0033 Oslo, Norway (Moldal); Department of Animal Medicine and Surgery, the Veterinary Faculty, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain (Tvarijonaviciute); and Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 108, 3584 CM Utrecht, Netherlands (Kirpensteijn).
Sabrina
S
Jakobsen
Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Groennegaardsvej 3 ground floor, Frederiksberg, Denmark (Christensen, Langhorn, Andreasen, Jacobsen, Persson, Kjelgaard-Hansen); Department of Companion Animal Clinical Studies, University of Pretoria, Private Bag X04, Onderstepoort, 0110, Pretoria, South Africa (Goddard); Department of Companion Animal Sciences, Norwegian School of Veterinary Science, P.O. Box 8146 Dep. N-0033 Oslo, Norway (Moldal); Department of Animal Medicine and Surgery, the Veterinary Faculty, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain (Tvarijonaviciute); and Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 108, 3584 CM Utrecht, Netherlands (Kirpensteijn).
Frida
F
Persson
Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Groennegaardsvej 3 ground floor, Frederiksberg, Denmark (Christensen, Langhorn, Andreasen, Jacobsen, Persson, Kjelgaard-Hansen); Department of Companion Animal Clinical Studies, University of Pretoria, Private Bag X04, Onderstepoort, 0110, Pretoria, South Africa (Goddard); Department of Companion Animal Sciences, Norwegian School of Veterinary Science, P.O. Box 8146 Dep. N-0033 Oslo, Norway (Moldal); Department of Animal Medicine and Surgery, the Veterinary Faculty, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain (Tvarijonaviciute); and Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 108, 3584 CM Utrecht, Netherlands (Kirpensteijn).
Mads
M
Kjelgaard-Hansen
D000818:Animals; D015415:Biomarkers; D002097:C-Reactive Protein / Q000378:metabolism*; D004283:Dog Diseases / Q000097:blood* / Q000378:metabolism; D004285:Dogs; D007249:Inflammation / Q000378:metabolism / Q000662:veterinary*; D011015:Pneumonia, Aspiration / Q000097:blood / Q000378:metabolism / Q000662:veterinary; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D000685:Serum Amyloid A Protein / Q000378:metabolism*; D012909:Snake Bites / Q000097:blood / Q000378:metabolism / Q000662:veterinary; D014947:Wounds and Injuries / Q000097:blood / Q000378:metabolism / Q000662:veterinary
D015415:Biomarkers; D000685:Serum Amyloid A Protein; D002097:C-Reactive Protein