Effect of E-beam treatment on the chemistry and on the antioxidant activity of lycopene from dry tomato peel and tomato powder.
Tomato powder (TP) and dry tomato peel (DTP) have been previously used in our laboratory as a source of lycopene to manufacture meat products ready-to-eat (RTE) submitted to E-beam irradiation with good technological and sensory results. Present work describes the studies performed in order to investigate the effect of radiation on chemical changes and antioxidant properties of lycopene. DTP and TP were irradiated (4 kGy). Changes on lycopene were analyzed by HPLC; inhibition of reactive oxygen species (ROS), possible modulation of mitogen-activated protein kinases (MAPK) cascade, nuclear factor κ-light-chain-enhancer of activated B cells (NP-κB) activation and expression of proteins involved in oxidation stress were analyzed in RAT-1 fibroblasts cell culture. Radiation reduced the content of all-E-lycopene and increased (Z)-lycopene, lycopene isomerization, and degradation being higher in DTP than in TP. E-Beam treatment increased the antioxidant ability of both DTP and TP in inhibiting spontaneous and H2O2-induced oxidative stress in cultured fibroblasts. Antioxidant activity was higher in DTP than in TP samples.
Department of Nutrition, Bromatology and Food Technology, Faculty of Veterinary, Complutense University , 28040 Madrid, Spain.
M Carmen
MC
Gámez
Marta M
MM
Calvo
M Dolores
MD
Selgas
M Luisa
ML
García
Katrin
K
Erler
Volker
V
Böhm
Assunta
A
Catalano
Rossella
R
Simone
Paola
P
Palozza
D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D002338:Carotenoids / Q000737:chemistry* / Q000494:pharmacology; D002460:Cell Line; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005514:Food Irradiation; D000077276:Lycopene; D018551:Solanum lycopersicum / Q000737:chemistry* / Q000528:radiation effects*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D011208:Powders / Q000737:chemistry / Q000528:radiation effects; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
D000975:Antioxidants; D010936:Plant Extracts; D011208:Powders; D017382:Reactive Oxygen Species; D002338:Carotenoids; D000077276:Lycopene