Aptamer-functionalized silver nanoparticles for scanometric detection of platelet-derived growth factor-BB.
In this work, we reported a scanometric assay system based on the aptamer-functionalized silver nanoparticles (apt-AgNPs) for detection of platelet-derived growth factor-BB (PDGF-BB) protein. The aptamer and ssDNA were bound with silver nanoparticles by self-assembly of sulfhydryl group at 5' end to form the apt-AgNPs probe. The apt-AgNPs probe can catalyze the reduction of metallic ions in color agent to generate metal deposition that can be captured both by human eyes and a flatbed scanner. Two different color agents, silver enhancer solution and color agent 1 (10 mM HAuCl4+2 mM hydroquinone) were used to develop silver and gold shell on the surface of AgNPs separately. The results demonstrated that the formation of Ag core-Au shell structure had some advantages especially in the low concentrations. The apt-AgNPs probe coupled with color agent 1 showed remarkable superiority in both sensitivity and detection limit compared to the apt-AuNPs system. The apt-AgNPs system also produced a wider linear range from 1.56 ng mL(-1) to 100 ng mL(-1) for PDGF-BB with the detection limit lower than 1.56 ng mL(-1). The present strategy was applied to the determination of PDGF-BB in 10% serum, and the results showed that it had good specificity in complex biological media.
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, PR China.
Hongting
H
Hu
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, PR China.
Hui
H
Li
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, PR China.
Yaju
Y
Zhao
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, PR China.
Shiyu
S
Dong
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, PR China.
Wei
W
Li
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, PR China.
Weibing
W
Qiang
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, PR China. Electronic address: xudanke@nju.edu.cn.
Danke
D
Xu
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D000077214:Becaplermin; D053768:Metal Nanoparticles / Q000737:chemistry*; D008969:Molecular Sequence Data; D020574:Proto-Oncogene Proteins c-sis / Q000032:analysis*; D012834:Silver / Q000737:chemistry*
D052157:Aptamers, Nucleotide; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D012834:Silver
Aptamer; Catalysis; Gold nanoparticles; Scanometric detection; Silver nanoparticles