[Modified HPLC method of determination of the valproic acid in biological fluids].
Proposed modified HPLC method for determination of valproic acid in biological fluids. Created solid-phase extraction of valproic and heptanoic acids (internal standard, IS) on the cartridges packed hyper cross-linked polystyrene which maintain some tens extractions without losses of efficiency. Carboxylic acids are derivative with 1-(bromoacetyl)pyrene in acetone at presence of triethylamine. Chromatographic separation of derivatives is performed on Chromolith Perfonnance RP-18e columns, which packed unique monolithic sorbent. UV detection at 360 nm. Mobile phase acetonitrile - water (90:10, v/v) plus 1% isopropanol, speed flow 2000 microL/min, pressure 21 bar. Complete chromatographic cycle less than 3 minutes. Yield of IS and valproic acid (extraction plus derivatization) was 101-106%. Sensitivity (limit detection) was near 1 ng for valproic and near 0.6 ng for heptanoic acid during signal/noise ratio = 3.
A A
AA
Dutov
D A
DA
Nikitin
V I
VI
Letunov
P P
PP
Tereshkov
O N
ON
Konovalova
D019840:2-Propanol / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D000293:Adolescent; D000328:Adult; D000927:Anticonvulsants / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004827:Epilepsy / Q000188:drug therapy / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D014635:Valproic Acid / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D014867:Water / Q000737:chemistry
D000097:Acetonitriles; D000927:Anticonvulsants; D014867:Water; D014635:Valproic Acid; D019840:2-Propanol; C032159:acetonitrile