Development of coronal cementum in hypsodont horse cheek teeth.
The horse is a grazing herbivore whose cheek teeth are hypsodon; that is, they possess long crowns that are completely covered by coronal cement at eruption. For elucidation of the sequential events in the formation of this coronal cementum in the mandibular horse cheek teeth, in the present study the lower 3rd permanent premolar teeth (PM4 ) from 3.5-, 4-, and 5-year-old horses were compared by using radiography, microcomputed tomography (Miro-CT), light microscopy (LM), and scanning electron microscopy (SEM). The present study clearly showed that prior to coronal cementogenesis tartrate-resistant acid phosphatase (TRAP)-positive odontoclasts resorbed on the enamel surface of the reserve crown in horse cheek tooth. Enamel resorption areas were relatively narrow, and started from the cuspal tips, and moved in the apical direction during tooth development. A primary cementum was initially deposited on the irregularly pitted enamel-cementum junction (ECJ) of the infolding and peripheral enamel. The infolding cementum filled grooves completely by the time of tooth eruption. On the other hand, in the peripheral cementum, the secondary and tertiary cementum layers were sequentially deposited on the primary cementum. These two cementum layers were sites for the insertion of the periodontal ligaments, and were continually laid down on the primary cementum coronally rather than apically throughout the life. The results of the present study suggest that the coronal cementum of horse cheek teeth is a multistructural and multifunctional tissue, meeting the requirements of its many different functions.
Department of Hard Tissue Research, Graduate School of Oral Medicine, Matsumoto Dental University, 1780 Gobara Hirooka Shiojiri, Nagano, 399-0781, Japan.
Noriyuki
N
Sahara
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D003739:Dental Cementum / Q000033:anatomy & histology* / Q000166:cytology / Q000648:ultrastructure; D003743:Dental Enamel / Q000166:cytology / Q000502:physiology; D006736:Horses / Q000033:anatomy & histology* / Q000254:growth & development; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D008855:Microscopy, Electron, Scanning; D010010:Osteoclasts / Q000166:cytology; D000071681:Tartrate-Resistant Acid Phosphatase; D014070:Tooth / Q000033:anatomy & histology* / Q000254:growth & development* / Q000648:ultrastructure; D055114:X-Ray Microtomography
D007527:Isoenzymes; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase
coronal cementogenesis; enamel resorption; horse; mandibular cheek teeth; odontoclasts