Tissue-specific antioxidant responses in pale chub (Zacco platypus) exposed to copper and benzo[a]pyrene.
In this study, antioxidant responses including lipid peroxidation (LPO), superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST), were evaluated in the liver, gill and muscle tissues of pale chub (Zacco platypus) exposed to copper (Cu) and benzo[a]pyrene (BaP). Cu exposure induced significant antioxidant responses in Z. platypus, particularly in the liver, whereas BaP exposure had a negligible effect. Following Cu exposure, both SOD and CAT activity increased in a concentration-dependent manner, showing significant correlations with malondialdehyde (MDA) levels as a measure of LPO (r = 0.646 and 0.663, respectively). SOD, CAT and GST mRNA levels were also enhanced following Cu exposure, except at 20 μg L(-1), although significant correlations with antioxidant enzyme activities were not found. The results of this study suggest that combined information on SOD and CAT activities together with LPO levels in the liver could be a useful indicator for assessing oxidative stress in freshwater fish.
Ecotoxicology Research Center, Korea Institute of Toxicology, Daejeon, 305-343, Republic of Korea.
Woo-Keun
WK
Kim
June-Woo
JW
Park
Eun-Suk
ES
Lim
Sung-Kyu
SK
Lee
Jungkon
J
Kim
Sunmi
S
Kim
Sang-Woo
SW
Lee
Kyungho
K
Choi
Jinho
J
Jung
D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D003300:Copper / Q000633:toxicity*; D003530:Cyprinidae / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D014874:Water Pollutants, Chemical; D001564:Benzo(a)pyrene; D008315:Malondialdehyde; D003300:Copper; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase