Single cell transfection with single molecule resolution using a synthetic nanopore.
We report the development of a single cell gene delivery system based on electroporation using a synthetic nanopore, that is not only highly specific and very efficient but also transfects with single molecule resolution at low voltage (1 V) with minimal perturbation to the cell. Such a system can be used to control gene expression with unprecedented precision--no other method offers such capabilities.
Department of Electrical Engineering, University of Notre Dame , Notre Dame, Indiana 46556, United States.
Volker
V
Kurz
Tetsuya
T
Tanaka
Gregory
G
Timp
D002462:Cell Membrane / Q000737:chemistry*; D004058:Diffusion; D018274:Electroporation / Q000379:methods*; D006801:Humans; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D058608:Nanopores / Q000648:ultrastructure*; D010316:Particle Size; D010957:Plasmids / Q000008:administration & dosage / Q000737:chemistry* / Q000235:genetics; D017655:Silicon Compounds / Q000737:chemistry*
D053769:Nanocapsules; D017655:Silicon Compounds; C032734:silicon nitride