Preparation of PLLA/bpV(pic) microspheres and their effect on nerve cells.
In this study, we prepared PLLA/bpV(pic) microspheres, a bpV(pic) controlled release system and examined their ability to protect nerve cells and promote axonal growth. PLLA microspheres were prepared by employing the o/w single emulsification-evaporation technique. Neural stem cells and dorsal root ganglia were divided into 3 groups in terms of the treatment they received: a routine medium group (cultured in DMEM), a PLLA microsphere group (DMEM containing PLLA microspheres alone) and a PLLA/bpV(pic) group [DMEM containing PLLA/bpV(pic) microspheres]. The effects of PLLA/bpV(pic) microspheres were evaluated by the live-dead test and measurement of axonal length. Our results showed that PLLA/bpV(pic) granulation rate was (88.2±5.6)%; particle size was (16.8±3.1)%, drug loading was (4.05±0.3)%; encapsulation efficiency was (48.5±1.8)%. The release time lasted for 30 days. In PLLA/bpV(pic) microsphere group, the cell survival rate was (95.2 ±4.77)%, and the length of dorsal root ganglion (DRG) was 718±95 μm, which were all significantly greater than those in ordinary routine medium group and PLLA microsphere group. This preliminary test results showed the PLLA/bpV(pic) microspheres were successfully prepared and they could promote the survival and growth of neural cells in DRG.
The Second Department of Orthopedic Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, 510006, China.
Qiang
Q
Lin
The Second Department of Orthopedic Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, 510006, China. drchygz@163.com.
Hai-Yun
HY
Chen
The Second Department of Orthopedic Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, 510006, China.
Hao-Shen
HS
Li
The Second Department of Orthopedic Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, 510006, China.
Yang-Ting
YT
Cai
D000818:Animals; D001369:Axons / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D004339:Drug Compounding; D005260:Female; D005727:Ganglia, Spinal / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D007150:Immunohistochemistry; D019344:Lactic Acid / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D008854:Microscopy, Electron; D008863:Microspheres; D058953:Neural Stem Cells / Q000187:drug effects / Q000502:physiology; D016900:Neurofilament Proteins / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D009942:Organometallic Compounds / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D011247:Pregnancy; D051381:Rats
D003692:Delayed-Action Preparations; D016900:Neurofilament Proteins; D009942:Organometallic Compounds; D011091:Polyesters; D011108:Polymers; C119540:oxodiperoxo(pyridine-2-carboxylate)vanadate(V); C051774:neurofilament protein H; D019344:Lactic Acid; C033616:poly(lactide)