The use of mesenchymal stem cells in bladder augmentation.
OBJECTIVE
To compare integration of bladder acellular matrix (BAM) with the bladder when seeded with mesenchymal stem cells (MSC) and when MSC are injected intravenously (IV).


METHODS
MSCs were isolated from bone marrow of EPM-1 Wistar male rats. Female rats were distributed into: Group A-BAM augmentation; Group B-BAM augmentation and MSCs IV administered; Group C-BAM-MSC seeded augmentation. Animals were killed on postoperative days 7, 14 and 28. Morphological analyses were performed using hematoxylin and eosin and Masson's trichrome, in addition to immunohistochemical staining with α-SMA and neurofilament for assessment of tissue repair. RNAm expression of the SRY gene was used to mark MSCs in the rats killed on postoperative day 28.


RESULTS
The muscle layer was best repaired in Groups B and C. No difference in the repair of the urothelium in the animals in any of the three groups was found. Group B presented the smallest inflammatory reaction and the best neural repair on postoperative day 28. None of the animals examined had MSCs in their bladder graft.


CONCLUSIONS
The MSCs were able to improve repair of the muscle layer and when injected intravenously, they were noted to initiate the neuronal regeneration process.
Department of Surgery, Federal University of São Paulo, Rua Batista Cepelos 87-ap 61, São Paulo, 04109-120, Brazil, milatorii@gmail.com.
Mila Torii Corrêa
MT
Leite
Luiz G
LG
Freitas-Filho
Andréia Silva
AS
Oliveira
Patrícia
P
Semedo-Kuriki
Marcus
M
Laks
Victor Eduardo Arrua
VE
Arias
Pedro S
PS
Peixoto
D000818:Animals; D005260:Female; D007275:Injections, Intravenous; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D001743:Urinary Bladder / Q000601:surgery*