[Study on different factors affecting the bionic enzymatic hydrolysis of icariin].
This study aims to observe different factors which affected the bionic enzymatic hydrolysis of icariin into baohuoside I and to optimize the reaction conditions in order to provide research foundation for building a novel bionic enzymolysis drug delivery system. To simulate the environment in vivo, 37 degrees C was set as the temperature and artificial intestinal juice and gastric juice were selected as the buffer solutions. Taking the conversion of baohuoside I as index, the effects of the kinds of enzyme, enzyme activity, substrate concentration, reaction time, pancreatin in artificial intestinal juice and surfactant on the conversion of baohuoside I were investigated. The results showed that cellulase, beta-glucosidase and snailase were all inactive in artificial gastric juice and no baohuoside I generated. Pancreatin in artificial intestinal juice couldn't significantly influence the activity of beta-glucosidase or snailase (P > 0.05), but noticeably decrease the activity of cellulase (P < 0.05). In artificial intestinal juice, the conversion of baohuoside I was highest by using beta-glucosidase, and the optimum reaction conditions were determined as follows: enzyme activity 10 U x mL(-1), substrate concentration 1 mg x mL(-1), 3 g x L(-1) rhamnolipid and reaction time 3 h. Under this condition, the conversion of baohuoside I was 99.8%.
Affiliated Hospital on Intergration of Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210046, China.
Xia
X
Gao
Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China.
Yan
Y
Chen
Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China.
Ying
Y
Wang
Affiliated Hospital on Intergration of Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210046, China.
Wen-jie
WJ
Sun
Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China.
Xiao-bin
XB
Jia
D000818:Animals; D002480:Cellulase / Q000737:chemistry; D005419:Flavonoids / Q000096:biosynthesis* / Q000378:metabolism*; D006867:Hydrolases / Q000737:chemistry / Q000302:isolation & purification; D006868:Hydrolysis; D010194:Pancreatin / Q000737:chemistry; D012908:Snails / Q000201:enzymology; D013501:Surface-Active Agents / Q000737:chemistry; D001617:beta-Glucosidase / Q000737:chemistry*
D005419:Flavonoids; D013501:Surface-Active Agents; C060988:baohuoside I; D010194:Pancreatin; D006867:Hydrolases; D001617:beta-Glucosidase; D002480:Cellulase; C056599:icariin