[Primary co-culture of cortical neurons and astrocytes of new-born SD rats].
This study is to establish a simple and practical co-culture method of cortical neurons and astrocytes of rats. The cortex of the new-born SD rats was digested by 0.125% pancreatic enzyme, and the differential adherence was applied to obtain the mixed cell suspension of neurons and astrocytes. A low concentration of cytarabine was used to inhibit the astrocytes in a moderate way to get neuronal and astrocyte co-culture. The morphological characteristics of the cells in different times were observed under the inverted microscope. The cells began to adhere the wall 2 h after the inoculation. Neurons and astrocytes grew in a good condition under the inverted microscope 9 days after the inoculation. The results of the immunofluorescence staining and Rosenfeld's staining indicated that the co-culture of neurons and astrocytes was successful and the ratio of neurons and astrocytes was close to 1:1. A new neurons and astrocytes co-culture method, which is simple and convenient, was successfully established. It will be an efficient method for the related researches about neuronal and astrocyte co-culture in vitro.
School of Life Science and Biopharmaceutic, Shenyang Pharmaceutical University, Shenyang 110016, China.
Cheng-na
CN
Wang
School of Life Science and Biopharmaceutic, Shenyang Pharmaceutical University, Shenyang 110016, China.
Li
L
Lin
School of Life Science and Biopharmaceutic, Shenyang Pharmaceutical University, Shenyang 110016, China.
Zhen-fang
ZF
Duan
School of Life Science and Biopharmaceutic, Shenyang Pharmaceutical University, Shenyang 110016, China.
Fei
F
Zhong
School of Life Science and Biopharmaceutic, Shenyang Pharmaceutical University, Shenyang 110016, China.
Dai-ying
DY
Zuo
School of Life Science and Biopharmaceutic, Shenyang Pharmaceutical University, Shenyang 110016, China.
Ying-liang
YL
Wu
D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000166:cytology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D018920:Coculture Techniques / Q000379:methods*; D005260:Female; D008297:Male; D009474:Neurons / Q000166:cytology*; D061251:Primary Cell Culture / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley