Histone deacetylase inhibitor sodium butyrate promotes the osteogenic differentiation of rat adipose-derived stem cells.
Adult stem cells hold great promise for use in tissue repair and regeneration. Recently, adipose tissue-derived stem cells (ADSCs) were found to be an appealing alternative to bone marrow stem cells (BMSCs) for bone tissue engineering. The main benefit of ADSCs is that they can be easily and abundantly available from adipose tissue. However, our prior study discovered an important phenomenon that BMSCs have greater osteogenic potential than ADSCs in vitro and epigenetic regulation plays a critical role in runt-related transcription factor 2 (Runx2) expression and thus osteogenesis. In this study, we aimed to improve the osteogenic potential of ADSCs by histone deacetylase inhibitor sodium butyrate (NaBu). We found that NaBu promoted rat ADSC osteogenic differentiation by altering the epigenetic modifications on the Runx2 promoter.
Institute of Sports Medicine, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing, 100191, China.
Xiaoqing
X
Hu
Yutuo
Y
Fu
Xin
X
Zhang
Linghui
L
Dai
Jingxian
J
Zhu
Zhenggang
Z
Bi
Yingfang
Y
Ao
Chunyan
C
Zhou
D000273:Adipose Tissue / Q000166:cytology*; D000704:Analysis of Variance; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D015153:Blotting, Western; D020148:Butyric Acid / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D047369:Chromatin Immunoprecipitation; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D044127:Epigenesis, Genetic / Q000187:drug effects*; D005434:Flow Cytometry; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D010012:Osteogenesis / Q000502:physiology*; D011401:Promoter Regions, Genetic / Q000235:genetics; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000502:physiology*
D050659:Core Binding Factor Alpha 1 Subunit; D017931:DNA Primers; D056572:Histone Deacetylase Inhibitors; C493717:Runx2 protein, rat; D020148:Butyric Acid
adipose derived stem cells; epigenetic regulation; histone deacetylase inhibitor; osteogenic differentiation; sodium butyrate