Pentadecapeptide BPC 157 and anaphylactoid reaction in rats and mice after intravenous dextran and white egg administration.
Anesthetized mice or rats received intravenously 6%, 10%, 20%, 40%, 60%, 80%, and 90% dextran and/or white egg (1ml/rat or 0.15ml/mouse) into their tails. Medication (/kg b.w., 5ml/kg) was given intraperitoneally (BPC 157 10µg, 1µg, 10ng, and 10pg/kg, chloropyramine 20mg/kg, and cimetidine 10mg/kg intraperitoneally, alone or in combination while controls received an equivolume of saline), immediately after challenge or, alternatively, at 5min after or 24 or 48h before challenge. The effect was assessed at 5, 10, 20 and 30min after dextran and/or white egg challenge. We commonly noted prominent edema involving the face, upper and lower lip, snout, paws and scrotum (presented with extreme cyanosis), poor respiration and the number of fatalities after dextran and/or white egg application. Contrary, BPC 157 regimens (10µg, 1µg, 10ng, and 10pg/kg) effectively, may both prevent anaphylactoid reactions that may arise from dextran and/or white egg application and furthermore, rescue already advanced reactions when given after the challenge. Chloropyramine and cimetidine given alone were only moderately effective. When given together with BPC 157, the observed effect correlates with the strong effect of BPC 157 given alone.
Department of Pharmacology, School of Medicine, University of Zagreb, Salata 11, 10000 Zagreb, Croatia.
Bozidar
B
Duplancic
Department of Pharmacology, School of Medicine, University of Zagreb, Salata 11, 10000 Zagreb, Croatia.
Vasilije
V
Stambolija
Department of Pharmacology, School of Medicine, University of Zagreb, Salata 11, 10000 Zagreb, Croatia.
Jadranka
J
Holjevac
Department of Pharmacology, School of Medicine, University of Zagreb, Salata 11, 10000 Zagreb, Croatia.
Mladen
M
Zemba
Department of Pharmacology, School of Medicine, University of Zagreb, Salata 11, 10000 Zagreb, Croatia.
Igor
I
Balenovic
Department of Pharmacology, School of Medicine, University of Zagreb, Salata 11, 10000 Zagreb, Croatia.
Domagoj
D
Drmic
Department of Pharmacology and Toxicology, The Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000, Zagreb, Croatia.
Jelena
J
Suran
Department of Pharmacology, School of Medicine, University of Zagreb, Salata 11, 10000 Zagreb, Croatia.
Bozo
B
Radic
Department of Pharmacology, School of Medicine, University of Zagreb, Salata 11, 10000 Zagreb, Croatia.
Marinko
M
Filipovic
Department of Pharmacology, School of Medicine, University of Zagreb, Salata 11, 10000 Zagreb, Croatia.
Alenka Boban
AB
Blagaic
Department of Pathology, School of Medicine, University of Zagreb, Salata 11, 10000 Zagreb, Croatia.
Luka
L
Brcic
Department of Pathology, School of Medicine, University of Zagreb, Salata 11, 10000 Zagreb, Croatia.
Danijela
D
Kolenc
Department of Pathology, The Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia.
Zeljko
Z
Grabarevic
Department of Pathology, School of Medicine, University of Zagreb, Salata 11, 10000 Zagreb, Croatia.
Sven
S
Seiwerth
Department of Pharmacology, School of Medicine, University of Zagreb, Salata 11, 10000 Zagreb, Croatia. Electronic address: sikiric@mef.hr.
Predrag
P
Sikiric
D061605:Administration, Intravenous; D000707:Anaphylaxis / Q000139:chemically induced / Q000517:prevention & control*; D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D002927:Cimetidine / Q000494:pharmacology; D003911:Dextrans; D004195:Disease Models, Animal; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control; D004529:Egg White; D005029:Ethylenediamines / Q000494:pharmacology; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D006635:Histamine H2 Antagonists / Q000494:pharmacology; D008297:Male; D051379:Mice; D010446:Peptide Fragments / Q000494:pharmacology*; D011506:Proteins / Q000494:pharmacology*; D017208:Rats, Wistar; D013997:Time Factors
D018926:Anti-Allergic Agents; D003911:Dextrans; D005029:Ethylenediamines; D006634:Histamine H1 Antagonists; D006635:Histamine H2 Antagonists; D010446:Peptide Fragments; D011506:Proteins; C004664:chloropyramine; D002927:Cimetidine; C083833:BPC 157
Anaphylactoid reaction; Dextran; Ovalbumin; Pentadecapeptide BPC 157