Prevalence and characterization of Staphylococcus aureus and Staphylococcus pseudintermedius isolated from companion animals and environment in the veterinary teaching hospital in Zambia, Africa.
The Republic of Zambia consists of only one veterinary teaching school at the University of Zambia (UNZA) where students and veterinarians are exposed to many bacterial pathogens including Staphylococcus aureus (SA) and Staphylococcus pseudintermedius (SP). The aim of this study was the characterization and antimicrobial susceptibility profile of eleven SA and 48 SP isolates from the veterinary hospitals' in- and outpatients and the environment. No isolate was resistant to cefoxitin by disk diffusion test and the corresponding resistance gene mecA was not found. In contrast, the resistance rates of SA to penicillin (63.6%) and trimethoprim-sulfamethoxazole (36.4%) and SP to penicillin (52.1%) and tetracycline (25.0%) were the highest. A variety of sequence types (STs) without a predominant type including numerous novel types were determined, especially for SP (39.6%). The spa typing provided a clonal assignment for all SAs (100%) and 24 SPs (50%) with three and two novel types, respectively. This study has provided an overview of SA and SP in the veterinary teaching hospital at UNZA. However, for a better understanding of these species regarding pathogenesis and transmission, further studies on the prevalence and characterization of SA and SP from veterinary staff, pet owners, and farm animals in Zambia is needed.
Division of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, Kita-20, Nishi-10, Kita-ku, Sapporo, Hokkaido 001-0020, Japan.
Jung-Ho
JH
Youn
Department of Microbiology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Republic of Korea.
Yong Ho
YH
Park
Department of Paraclinical Studies, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka, Zambia.
Bernard
B
Hang'ombe
Division of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, Kita-20, Nishi-10, Kita-ku, Sapporo, Hokkaido 001-0020, Japan. Electronic address: sugimoto@czc.hokudai.ac.jp.
Chihiro
C
Sugimoto
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D002371:Cat Diseases; D002415:Cats; D004269:DNA, Bacterial / Q000235:genetics; D004283:Dog Diseases; D004285:Dogs; D024881:Drug Resistance, Bacterial; D006762:Hospitals, Animal; D006784:Hospitals, Teaching; D008969:Molecular Sequence Data; D010406:Penicillins / Q000494:pharmacology; D057805:Pets; D015995:Prevalence; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D013210:Staphylococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D013211:Staphylococcus aureus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D013752:Tetracycline / Q000494:pharmacology; D015662:Trimethoprim, Sulfamethoxazole Drug Combination / Q000494:pharmacology; D015024:Zambia / Q000453:epidemiology
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D010406:Penicillins; D015662:Trimethoprim, Sulfamethoxazole Drug Combination; D013752:Tetracycline
Africa; Antimicrobial resistance; Staphylococcus aureus; Staphylococcus pseudintermedius; Veterinary hospital