[Urine metabonomic study of intervention effects of Morinda officinalis how. on 'kidney-yang deficiency syndrome'].
To investigate the intervention effects of Morinda officinalis How. on 'Kidney-yang deficiency syndrome' induced by hydrocortisone in rats, the metabolic profiles of rat urine were characterized using proton nuclear magnetic resonance and principal component analysis (PCA) was applied to study the trajectory of urinary metabolic phenotype of rats with 'Kidney-yang deficiency syndrome' under administration of M. officinalis at different time points. Meanwhile, the intervention effects of M. officinalis on urinary metabolic potential biomarkers associated with 'Kidney-yang deficiency syndrome' were also discussed. The experimental results showed that in accordance to the increased time of administration, an obvious tendency was observed that clustering of the treatment group moved gradually closed to that of the control group. Eight potential biomarkers including citrate, succinate, alpha-ketoglutarate, lactate, betaine, sarcosine, alanine and taurine were definitely up- or down-regulated. In conclusion, the effectiveness of M. oficinalis on 'Kidney-yang deficiency syndrome' is proved using the established metabonomic method and the regulated metabolic pathways involve energy metabolism, transmethylation and transportation of amine. Meanwhile, the administration of M. officinalis can alleviate the kidney impairment induced by 'Kidney-yang deficiency syndrome'.
College of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China. zouzhongjie@139.com
Zhong-jie
ZJ
Zou
Department of Chemistry, Tsinghua University, Beijing 100084, China.
Yuan-yuan
YY
Xie
College of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Meng-juan
MJ
Gong
College of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Bin
B
Han
College of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Shu-mei
SM
Wang
College of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Sheng-wang
SW
Liang
D000409:Alanine / Q000652:urine; D000818:Animals; D001622:Betaine / Q000652:urine; D015415:Biomarkers / Q000652:urine*; D019343:Citric Acid / Q000652:urine; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D006854:Hydrocortisone; D007656:Ketoglutaric Acids / Q000652:urine; D007674:Kidney Diseases / Q000139:chemically induced / Q000652:urine*; D019344:Lactic Acid / Q000652:urine; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D055432:Metabolomics / Q000379:methods; D032066:Morinda / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D025341:Principal Component Analysis; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012521:Sarcosine / Q000652:urine; D019802:Succinic Acid / Q000652:urine; D013654:Taurine / Q000652:urine; D016711:Yang Deficiency / Q000139:chemically induced / Q000652:urine*
D015415:Biomarkers; D004365:Drugs, Chinese Herbal; D007656:Ketoglutaric Acids; D013654:Taurine; D019343:Citric Acid; D019344:Lactic Acid; D001622:Betaine; D019802:Succinic Acid; D000409:Alanine; D006854:Hydrocortisone; D012521:Sarcosine