Live cell cytoplasm staining and selective labeling of intracellular proteins by non-toxic cell-permeant thiophene fluorophores.
A structurally correlated series of cell-permeant thiophene fluorophores, characterized by intense green or red fluorescence inside live mouse embryonic fibroblasts, was developed. The fluorophores displayed rapid internalization, excellent retention inside the cells, and high optical stability in the cytosolic environment and did not alter cell viability and reproducibility. Depending on the molecular structure, they experienced distinct fate inside the cells: from bright and lasting staining of the cytoplasm to selective tagging of a small set of globular proteins.
Laboratorio MIST.E-R, National Research Council, Via Gobetti 101, I-40129 Bologna, Italy.
F
F
Di Maria
I E
IE
Palamà
M
M
Baroncini
A
A
Barbieri
A
A
Bongini
R
R
Bizzarri
G
G
Gigli
G
G
Barbarella
D000818:Animals; D002470:Cell Survival; D003593:Cytoplasm / Q000737:chemistry*; D005347:Fibroblasts / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D011506:Proteins / Q000032:analysis*; D013194:Staining and Labeling; D013876:Thiophenes / Q000138:chemical synthesis / Q000737:chemistry*
D005456:Fluorescent Dyes; D011506:Proteins; D013876:Thiophenes