Sulfite oxidase biosensors based on tetrathiafulvalene modified screen-printed carbon electrodes for sulfite determination in wine.
Screen-printed carbon electrodes have been modified with tetrathiafulvalene and sulfite oxidase enzyme for the sensitive and selective detection of sulfite. Amperometric experimental conditions were optimized taking into account the importance of quantifying sulfite in wine samples and the inherent complexity of these samples, particularly red wine. The biosensor responds to sulfite giving a cathodic current (at +200 mV vs screen-printed Ag/AgCl electrode and pH 6) in a wide concentration range, with a capability of detection of 6 μM (α=β=0.05) at 60°C. The method has been applied to the determination of sulfite in white and red samples, with averages recoveries of 101.5% to 101.8%, respectively.
Analytical Chemistry Department, Faculty of Sciences, University of Burgos, Pza. Misael Bañuelos s/n, 09001 Burgos, Spain.
Begoña
B
Molinero-Abad
Analytical Chemistry Department, Faculty of Sciences, University of Burgos, Pza. Misael Bañuelos s/n, 09001 Burgos, Spain. Electronic address: malomillo@ubu.es.
M Asunción
MA
Alonso-Lomillo
Analytical Chemistry Department, Faculty of Sciences, University of Burgos, Pza. Misael Bañuelos s/n, 09001 Burgos, Spain.
Olga
O
Domínguez-Renedo
Analytical Chemistry Department, Faculty of Sciences, University of Burgos, Pza. Misael Bañuelos s/n, 09001 Burgos, Spain.
M Julia
MJ
Arcos-Martínez
D015374:Biosensing Techniques; D002244:Carbon; D004566:Electrodes; D006801:Humans; D011994:Recombinant Proteins / Q000378:metabolism; D050876:Sulfite Oxidase / Q000378:metabolism*; D013447:Sulfites / Q000032:analysis*; D014920:Wine / Q000032:analysis*
D011994:Recombinant Proteins; D013447:Sulfites; D002244:Carbon; D050876:Sulfite Oxidase
Screen-printed carbon electrodes; Sulfite; Sulfite oxidase; Tetrathiafulvalene; Wine