Detection of Caprine-specific Nucleic Acid Sequences in Goat Milk Using Polymerase Chain Reaction.
BACKGROUND
none declared.


BACKGROUND
This study was carried out to evaluate PCR-based method for detection of DNA in goat milk. It utilized primers targeting the mitochondrial cytochrome -b (mtcyt-b) gene, which was used as a target DNA for PCR amplification.


METHODS
For the specific identification of goat mtcyt-b gene, pair of primers (GSL1, GSR2), were used, which produced a 428 base pair (bp) PCR product from milk samples as well as from peripheral blood. Amplification products were visualized on ethidium bromide-stained agarose gels.


CONCLUSIONS
Amplification products were not detected when the PCR was applied to DNA from animal species including cattle, sheep, swine, camel, deer, horse, donkey, and human, which indicates that the 2 pairs of primers are specific for goat.


CONCLUSIONS
DNA can be extracted from goat milk and would be advantageous in the variety of application such as species identification in milk and milk.
Molecular Biology Laboratory, Department of Clinical Medicine, Faculty of Veterinary Medicine, University Khartoum , Sudan ; Department of Physiology, Faculty of Medicine, Gadarif University , Gadarif, Sudan.
Asim A
AA
Osman
Molecular Biology Laboratory, Department of Clinical Medicine, Faculty of Veterinary Medicine, University Khartoum , Sudan.
Imadeldin E
IE
Aradaib
Department of Physiology, Faculty of Medicine, The National Ribat University , Khartoum, Sudan.
Omer A
OA
Musa
DNA; Goat milk; PCR assay; Rapid detection