Triple nanoemulsion potentiates the effects of topical treatments with microencapsulated retinol and modulates biological processes related to skin aging.
BACKGROUND
The sum of environmental and genetic factors affects the appearance and function of the skin as it ages. The identification of molecular changes that take place during skin aging provides biomarkers and possible targets for therapeutic intervention. Retinoic acid in different formulations has emerged as an alternative to prevent and repair age-related skin damage.


OBJECTIVE
To understand the effects of different retinoid formulations on the expression of genes associated with biological processes that undergo changes during skin aging.


METHODS
Ex-vivo skin samples were treated topically with different retinoid formulations. The modulation of biological processes associated with skin aging was measured by Reverse Transcription quantitative PCR (RT-qPCR).


RESULTS
A formulation containing microencapsulated retinol and a blend of active ingredients prepared as a triple nanoemulsion provided the best results for the modulation of biological, process-related genes that are usually affected during skin aging.


CONCLUSIONS
This association proved to be therapeutically more effective than tretinoin or microencapsulated retinol used singly.
Laboratório de Biologia Molecular, Grupo Boticário, CuritibaPR, Brazil, MSc - Biomolecular Specialist, Laboratório de Biologia Molecular (LABIM), Grupo Boticário - Curitiba (PR), Brazil.
Alessandro
A
Afornali
Laboratório de Biologia Molecular, Grupo Boticário, CuritibaPR, Brazil, MSc - Biomolecular Specialist, Laboratório de Biologia Molecular (LABIM), Grupo Boticário - Curitiba (PR), Brazil.
Rodrigo de
Rd
Vecchi
Laboratório de Biologia Molecular, Grupo Boticário, CuritibaPR, Brazil, PhD - Biomolecular Researcher, Laboratório de Biologia Molecular (LABIM), Grupo Boticário - Curitiba (PR), Brazil.
Rodrigo Makowiecky
RM
Stuart
Skingen Inteligência Genética, Grupo Boticário, CuritibaPR, Brazil, PhD - Technology Research Mananger, Skingen Inteligência Genética, Curitiba (PR), Grupo Boticário - Curitiba (PR), Brazil.
Gustavo
G
Dieamant
Skingen Inteligência Genética, CuritibaPR, Brazil, MSc - Technology Research, Skingen Inteligência Genética - Curitiba (PR), Brazil.
Luciana Lima de
LL
Oliveira
Laboratório de Biologia Molecular, Grupo Boticário, BrazilCuritibaPR, PhD - Biomolecular Research Coordinator, Laboratório de Biologia Molecular (LABIM), Grupo Boticário - Curitiba (PR), Brazil.
Carla Abdo
CA
Brohem
Skingen Inteligência Genética, CuritibaPR, Brazil, Pharm - Director, Skingen Inteligência Genética - Curitiba (PR), Brazil.
Israel Henrique Stokfisz
IH
Feferman
Department of Dermatology, Hospital Evangélico de Curitiba, Faculdade Evangélica do Paraná, CuritibaPR, Brazil, MD - Professor, Head of the Department of Dermatology at Hospital Evangélico de Curitiba - Faculdade Evangélica do Paraná (HUEC-FEPAR) - Curitiba (PR), Brazil.
Lincoln Helder Zambaldi
LH
Fabrício
Skingen Inteligência Genética, CuritibaPR, Brazil, MSc - Biomolecular Research Mananger, Skingen Inteligência Genética, Curitiba (PR), Brazil.
Márcio
M
Lorencini
D000287:Administration, Topical; D000704:Analysis of Variance; D001686:Biological Phenomena / Q000187:drug effects; D004357:Drug Synergism; D004655:Emulsions; D015870:Gene Expression; D006801:Humans; D007641:Keratolytic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D050997:Nanomedicine; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000187:drug effects*; D015595:Skin Aging / Q000187:drug effects* / Q000235:genetics; D014212:Tretinoin / Q000494:pharmacology / Q000627:therapeutic use*
D004655:Emulsions; D007641:Keratolytic Agents; D014212:Tretinoin