Preparation of synthetic oligosaccharide-conjugates of poly-β-(1→6)-N-acetyl glucosamine.
Staphylococcus aureus and Staphylococcus epidermidis are prominent bacterial pathogens of nosocomial infections. Both microorganisms colonize medical devices by forming adherent biofilms. Poly-β-D-(1→6)-N-acetyl-glucosamine (PNAG) is a surface polysaccharide antigen which was found on both S. aureus and S. epidermidis. Animal studies have proved that PNAG can elicit antibodies which protect against staphylococcal infections. We have presented the synthesis of di-, tetra- and hexasaccharide fragments of PNAG with formyl-heptyl aglycone and their attachment to bovine serum albumin (BSA) by reductive amination.
Department of Organic Chemistry, University of Debrecen, PO Box 20, H-4010 Debrecen, Hungary. Electronic address: fekete.aniko@science.unideb.hu.
Anikó
A
Fekete
Department of Pharmaceutical Chemistry, Medical and Health Center, University of Debrecen, PO Box 70, H-4010 Debrecen, Hungary.
Dániel
D
Eszenyi
Department of Organic Chemistry, University of Debrecen, PO Box 20, H-4010 Debrecen, Hungary.
Mihály
M
Herczeg
National Institute of Child Health and Human Development, National Institutes of Health, 31 Center Dr., MSC 2423 Bethesda, MD 20892-2423, United States.
Vince
V
Pozsgay
Department of Pharmaceutical Chemistry, Medical and Health Center, University of Debrecen, PO Box 70, H-4010 Debrecen, Hungary.
Anikó
A
Borbás
D000586:Amination; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D011135:Polysaccharides, Bacterial / Q000138:chemical synthesis; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D047071:beta-Glucans / Q000138:chemical synthesis*
D011135:Polysaccharides, Bacterial; D047071:beta-Glucans; C505465:poly-N-acetyl-1-6-glucosamine; D012710:Serum Albumin, Bovine
Carbohydrate chemical synthesis; Glycoconjugate; Poly-β-(1→6)-N-acetyl glucosamine; Reductive amination