Nar1 deficiency results in shortened lifespan and sensitivity to paraquat that is rescued by increased expression of mitochondrial superoxide dismutase.
Saccharomyces cerevisiae Nar1p is an essential Fe/S protein that exhibits striking similarity to bacterial iron-only hydrogenases. Nar1p is required for the maturation of cytosolic and nuclear, but not of mitochondrial Fe/S proteins, and plays a role in modulating sensitivity to oxygen in both yeast and Caenorhabditis elegans through unknown mechanisms. Here we report that Nar1 deficiency results in shortened lifespan and sensitivity to paraquat that is rescued by increased expression of mitochondrial superoxide dismutase. These data suggest that Nar1p promotes protection against oxidative stress and define a new role for Nar1p in promoting replicative lifespan.
Institute of Aging Research, Guangdong Medical College, Dongguan, China; Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan, China.
Wei
W
Zhao
Institute of Aging Research, Guangdong Medical College, Dongguan, China; Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan, China.
Bing Xiong
BX
Fang
Institute of Aging Research, Guangdong Medical College, Dongguan, China; Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan, China; Institute of Biochemistry & Molecular Biology, Guangdong Medical College, Zhanjiang, China.
Yu Jie
YJ
Niu
Institute of Aging Research, Guangdong Medical College, Dongguan, China; Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan, China.
Yi Na
YN
Liu
Institute of Aging Research, Guangdong Medical College, Dongguan, China; Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan, China.
Bin
B
Liu
Institute of Aging Research, Guangdong Medical College, Dongguan, China; Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan, China.
Qi
Q
Peng
Institute of Aging Research, Guangdong Medical College, Dongguan, China.
Jiang Bin
JB
Li
Department of Pathology, University of Washington, Seattle, USA.
Brian M
BM
Wasko
Department of Pathology, University of Washington, Seattle, USA.
Joseph R
JR
Delaney
Institute of Aging Research, Guangdong Medical College, Dongguan, China; Buck Institute for Research on Aging, Novato, USA.
Brian K
BK
Kennedy
Institute of Aging Research, Guangdong Medical College, Dongguan, China; Departments of Medicine and Genetics, Albert Einstein College of Medicine, Bronx, USA.
Yousin
Y
Suh
Institute of Aging Research, Guangdong Medical College, Dongguan, China; Department of Biochemistry, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong.
Zhong Jun
ZJ
Zhou
Institute of Aging Research, Guangdong Medical College, Dongguan, China; Department of Pathology, University of Washington, Seattle, USA.
Matt
M
Kaeberlein
Institute of Aging Research, Guangdong Medical College, Dongguan, China; Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan, China; Institute of Biochemistry & Molecular Biology, Guangdong Medical College, Zhanjiang, China. Electronic address: xgliu64@126.com.
Xin Guang
XG
Liu
D000818:Animals; D003600:Cytosol / Q000378:metabolism; D006540:Herbicides / Q000378:metabolism / Q000494:pharmacology; D006864:Hydrogenase / Q000378:metabolism; D007506:Iron-Sulfur Proteins / Q000172:deficiency / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology; D010269:Paraquat / Q000378:metabolism / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000502:physiology / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors
D006540:Herbicides; D007506:Iron-Sulfur Proteins; D029701:Saccharomyces cerevisiae Proteins; C118830:iron hydrogenase; D006864:Hydrogenase; C501975:Nar1 protein, S cerevisiae; D013482:Superoxide Dismutase; D010269:Paraquat
Lifespan; Mitochondrial superoxide dismutase; Nar1