Long lived BSA Au clusters as a time gated intensity imaging probe.
The work presented here reports the use of long lifetime (>1 μs) BSA Au clusters as a cellular/tissue, time gated, intensity imaging probe. By collecting the emission signal 50 ns post excitation, one can off-gate the intense auto-fluorescence background, thereby greatly enhancing the clarity/specificity in fluorescence imaging.
Center for Commercialization of Fluorescence Technologies, Department of Cell Biology and Immunology, University of North Texas Health Science Center, 3500 Camp Bowie Blvd., Fort Worth, Texas 76107, USA. sraut@live.unthsc.edu Ignacy.gryczynski@unthsc.edu.
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D000818:Animals; D002417:Cattle; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D006046:Gold / Q000737:chemistry*; D051379:Mice; D008856:Microscopy, Fluorescence; D049329:Nanostructures / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism
D005456:Fluorescent Dyes; D012710:Serum Albumin, Bovine; D006046:Gold