Hepatic Fibrosis Inhibitory Effect of Peptides Isolated from Navicula incerta on TGF-β1 Induced Activation of LX-2 Human Hepatic Stellate Cells.
In this study, novel peptides (NIPP-1, NIPP-2) derived from Navicula incerta (microalgae) protein hydrolysate were explored for their inhibitory effects on collagen release in hepatic fibrosis with the investigation of its underlying mechanism of action. TGF-β1 activated fibrosis in LX-2 cells was examined in the presence or absence of purified peptides NIPP-1 and NIPP-2. Besides the mechanisms of liver cell injury, protective effects of NIPP-1 and NIPP-2 were studied to show the protective mechanism against TGF-β1 stimulated fibrogenesis. Our results showed that the core protein of NIPP-1 peptide prevented fibril formation of type I collagen, elevated the MMP level and inhibited TIMP production in a dose-dependent manner. The treatment of NIPP-1 and NIPP-2 on TGF-β1 induced LX-2 cells alleviated hepatic fibrosis. Moreover, α-SMA, TIMPs, collagen and PDGF in the NIPP-1 treated groups were significantly decreased. Therefore, it could be suggested that NIPP-1 has potential to be used in anti-fibrosis treatment.
Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Korea.
Kyong-Hwa
KH
Kang
Oceanic Life Research Center, Chosun University, Jeonnam 543-700, Korea.
Zhong-Ji
ZJ
Qian
Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Korea.
Bomi
B
Ryu
Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Korea.
Fatih
F
Karadeniz
Marine Bio Research Team, Korea Basic Science Institute (KBSI), Jeju 690-140, Korea.
Daekyung
D
Kim
Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Korea ; Department of Chemistry, Pukyong National University, Busan 608-737, Korea.
Se-Kwon
SK
Kim
LX-2; hepatic fibrosis; hepatic stellate cells; liver injury; microalgae