[Primary study of skin wound healing in rats for Loropetalum chinens].
OBJECTIVE
Cutaneous wound is a common health problem of humans. Loropetalum chinens, a medicinal plant, is widely used to treat wounds among the people. The research aims to observe whether L. chinens can promote the rats' wounds healing process, isolate the extracts primarily and commit the wound healing selection, which provide work basis for wound healing research of L. chinens.


METHODS
First we analyzed the possible components with HC-MS/MS, then committed our wound healing experiments for L. chinens in the rat incision wound model and excision wound model, which are commonly used worldwide. After that, we carried on the preliminary isolation of the L. chinens and we screened the heal-promoting effects of the isolations in incision wound model.


RESULTS
L. chinens significantly accelerates the wound healing of rat's skin, shortens the healing period, enhances the healing intensity and promotes the cell proliferation and blood vessels formation around the wounds. The isolations, which are petroleum ether layer, ethyl acetate layer and n-butyl alcohol layer, exert heal-promoting effects. It indicates that the possible morphon that promotes wound healing may exist in these three components, with small polar.


CONCLUSIONS
L. chinens possesses strong wound healing promoting effects, and the active constituent, with small polar, exists in petroleum ether layer, ethyl acetate layer and n-butyl alcohol layer, and we should focus on these three layers when carrying on further studies.
State Key Laboratory of Bioactive Substance and Function of Natural Medicines & Ministry of Health Key Laboratory of Biosynthesis of Natural Products, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Ze-Qin
ZQ
Lian
State Key Laboratory of Bioactive Substance and Function of Natural Medicines & Ministry of Health Key Laboratory of Biosynthesis of Natural Products, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Jian
J
Gao
Jiangxi Deyu Group, Jingdezhen 333000, China.
Xiao-Bin
XB
Li
Jiangxi Deyu Group, Jingdezhen 333000, China.
Hao-Yuan
HY
Liu
State Key Laboratory of Bioactive Substance and Function of Natural Medicines & Ministry of Health Key Laboratory of Biosynthesis of Natural Products, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Hai-Bo
HB
Zhu
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D029201:Hamamelidaceae / Q000737:chemistry*; D006801:Humans; D008297:Male; D008517:Phytotherapy; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects* / Q000293:injuries / Q000503:physiopathology; D012871:Skin Diseases / Q000188:drug therapy* / Q000503:physiopathology; D014945:Wound Healing / Q000187:drug effects*
D004365:Drugs, Chinese Herbal