Engineering micropatterned surfaces to modulate the function of vascular stem cells.
Multipotent vascular stem cells have been implicated in vascular disease and in tissue remodeling post therapeutic intervention. Hyper-proliferation and calcified extracellular matrix deposition of VSC cause blood vessel narrowing and plaque hardening thereby increasing the risk of myocardial infarct. In this study, to optimize the surface design of vascular implants, we determined whether micropatterned polymer surfaces can modulate VSC differentiation and calcified matrix deposition. Undifferentiated rat VSC were cultured on microgrooved surfaces of varied groove widths, and on micropost surfaces. 10μm microgrooved surfaces elongated VSC and decreased cell proliferation. However, microgrooved surfaces did not attenuate calcified extracellular matrix deposition by VSC cultured in osteogenic media conditions. In contrast, VSC cultured on micropost surfaces assumed a dendritic morphology, were significantly less proliferative, and deposited minimal calcified extracellular matrix. These results have significant implications for optimizing the design of cardiovascular implant surfaces.
Department of Bioengineering, University of California, Berkeley, 121 Stanley Hall, Berkeley, CA 94720, United States.
Jennifer
J
Li
Department of Bioengineering, University of California, Berkeley, 121 Stanley Hall, Berkeley, CA 94720, United States.
Michelle
M
Wu
Department of Bioengineering, University of California, Berkeley, 121 Stanley Hall, Berkeley, CA 94720, United States.
Julia
J
Chu
Berkeley Sensor and Actuator Center, University of California, Berkeley, 668 Sutardja Dai Hall, Berkeley, CA 94720, United States.
Ryan
R
Sochol
Department of Bioengineering, University of California, Berkeley, 121 Stanley Hall, Berkeley, CA 94720, United States. Electronic address: shyampatel@berkeley.edu.
Shyam
S
Patel
D000818:Animals; D001011:Aorta / Q000166:cytology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D010012:Osteogenesis; D051381:Rats; D013234:Stem Cells / Q000166:cytology*; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry*
D004129:Dimethylpolysiloxanes; C013830:baysilon
Cardiovascular; Microgrooves; Micropatterning; Microposts; Vascular stem cells