Crude extract of Rheum palmatum L inhibits migration and invasion of LS1034 human colon cancer cells acts through the inhibition of matrix metalloproteinase-2/-9 by MAPK signaling.
Crude extract of Rheum palmatum L. (CERP) has been used to treat different diseases in the Chinese population for decades. In this study, we investigated the anti-metastasis effects of CERP on LS1034 human colorectal cancer cells in vitro and examined potential mechanisms of its effects. CERP significantly inhibited cell migration and invasion of LS1034 cells. We also found that CERP inhibited protein levels of matrix metalloproteinases-2 (MMP-2) and matrix metalloproteinases-9 (MMP-9), and cytosolic NF-kB p65, RHO A, ROCK 1. Furthermore, we found CERP inhibited protein levels of GRB2, SOS1, MKK7, FAK, Rho A, ROCK 1, VEGF, PKC, AKT, phosphor-AKT (Thr308), Cyclin D, iNOS, COX2, NF-kB p65, p-ERK1/2, p-JNK1/2, p-p38, p-c-jun, MMP-2, MMP-9, MMP-1, MMP-7, MMP-10, UPA and increased the protein level of Ras in LS1034 cells. In conclusion, our results suggest that CERP may be used as a novel anti-metastasis agent for the treatment of human colon cancer cells.
Graduate Institute of Chinese Medicine, China Medical University, No 91, Hsueh-Shih Road, Taichung, 402, Taiwan, Republic of China.|Department of Chinese Medicine, Changhua Hospital, Changhua, Taiwan, Republic of China.|Department of Health, Executive Yuan, Changhua, Taiwan, Republic of China.
Yi-Shih
YS
Ma
Departments of Dermatology, Chung Shan Medical University Hospital, Taichung, 402, Taiwan, Republic of China.|Institute of Medicine, Chung Shan Medical University, Taichung, 402, Taiwan, Republic of China.
Yu-Ping
YP
Hsiao
Departments of Biological Science and Technology, China Medical University, No 91, Hsueh-Shih Road, Taichung, 402, Taiwan, Republic of China.
Ju-Hwa
JH
Lin
Departments of Biological Science and Technology, China Medical University, No 91, Hsueh-Shih Road, Taichung, 402, Taiwan, Republic of China.
Shu-Chun
SC
Hsu
Department of Health and Nutrition Biotechnology, Asia University, Taichung, 413, Taiwan, Republic of China.
Fu-Shin
FS
Chueh
Graduate Institute of Chinese Medicine, China Medical University, No 91, Hsueh-Shih Road, Taichung, 402, Taiwan, Republic of China.|Department of Chinese Medicine, Taichung Hospital, Taichung, 403, Taiwan, Republic of China.|Department of Health, Executive Yuan, Taichung, 403, Taiwan, Republic of China.
Shu-Wen
SW
Weng
School of Medicine, China Medical University, No 91, Hsueh-Shih Road, Taichung, 402, Taiwan, Republic of China.|Department of Surgery, China Medical University Beigang Hospital, Yunlin, 651, Taiwan, Republic of China.
Kuang-Chi
KC
Lai
Graduate Institute of Chinese Medicine, China Medical University, No 91, Hsueh-Shih Road, Taichung, 402, Taiwan, Republic of China.
Jaung-Geng
JG
Lin
Departments of Biological Science and Technology, China Medical University, No 91, Hsueh-Shih Road, Taichung, 402, Taiwan, Republic of China.|Department of Biotechnology, Asia University, Taichung, 413, Taiwan, Republic of China.
Jing-Gung
JG
Chung
D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012250:Rheum / Q000737:chemistry* / Q000378:metabolism; D051996:Transcription Factor RelA / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects; D054460:rho-Associated Kinases / Q000378:metabolism; D020742:rhoA GTP-Binding Protein / Q000378:metabolism
D010936:Plant Extracts; D051996:Transcription Factor RelA; C515851:ROCK1 protein, human; D054460:rho-Associated Kinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D020742:rhoA GTP-Binding Protein
crude extract of Rheum palmatum L; human colon cancer LS1034 cells; invasion; matrix metalloproteinases; migration