Root carboxylate exudation capacity under phosphorus stress does not improve grain yield in green gram.
Genetic variability in carboxylate exudation capacity along with improved root traits was a key mechanism for P-efficient green gram genotype to cope with P-stress but it did not increase grain yield. This study evaluates genotypic variability in green gram for total root carbon exudation under low phosphorus (P) using (14)C and its relationship with root exuded carboxylates, growth and yield potential in contrasting genotypes. Forty-four genotypes grown hydroponically with low (2 μM) and sufficient (100 μM) P concentrations were exposed to (14)CO2 to screen for total root carbon exudation. Contrasting genotypes were employed to study carboxylate exudation and their performance in soil at two P levels. Based on relative (14)C exudation and biomass, genotypes were categorized. Carboxylic acids were measured in exudates and root apices of contrasting genotypes belonging to efficient and inefficient categories. Oxalic and citric acids were released into the medium under low-P. PDM-139 (efficient) was highly efficient in carboxylate exudation as compared to ML-818 (inefficient). In low soil P, the reduction in biomass was higher in ML-818 as compared to PDM-139. Total leaf area and photosynthetic rate averaged for genotypes increased by 71 and 41 %, respectively, with P fertilization. Significantly, higher root surface area and volume were observed in PDM-139 under low soil P. Though the grain yield was higher in ML-818, the total plant biomass was significantly higher in PDM-139 indicating improved P uptake and its efficient translation into biomass. The higher carboxylate exudation capacity and improved root traits in the later genotype might be the possible adaptive mechanisms to cope with P-stress. However, it is not necessary that higher root exudation would result in higher grain yield.
Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi, 110012, India, renu_iari@rediffmail.com.
Renu
R
Pandey
Surendra Kumar
SK
Meena
Vengavasi
V
Krishnapriya
Altaf
A
Ahmad
Naval
N
Kishora
D001692:Biological Transport; D018533:Biomass; D002244:Carbon / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002264:Carboxylic Acids / Q000378:metabolism*; D018556:Crops, Agricultural; D007887:Fabaceae / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D014644:Genetic Variation; D005838:Genotype; D010641:Phenotype; D010758:Phosphorus / Q000378:metabolism*; D010788:Photosynthesis; D053147:Plant Exudates / Q000378:metabolism*; D018515:Plant Leaves / Q000254:growth & development / Q000502:physiology; D018517:Plant Roots / Q000254:growth & development / Q000502:physiology; D018520:Plant Shoots / Q000254:growth & development / Q000502:physiology; D012639:Seeds / Q000254:growth & development / Q000502:physiology; D012987:Soil / Q000737:chemistry; D013312:Stress, Physiological
D002250:Carbon Radioisotopes; D002264:Carboxylic Acids; D053147:Plant Exudates; D012987:Soil; D010758:Phosphorus; D002244:Carbon