Screening of cellular proteins that interact with the classical swine fever virus non-structural protein 5A by yeast two-hybrid analysis.
Classical swine fever virus (CSFV), the pathogen of classical swine fever (CSF), causes severe hemorrhagic fever and vascular necrosis in domestic pigs and wild boar. A large number of evidence has proven that non-structural 5A (NS5A) is not only a very important part of viral replication complex, but also can regulate host cell's function; however, the underlying mechanisms remain poorly understood. In the current study, aiming to find more clues in understanding the molecular mechanisms of CSFV NS5A's function, the yeast two-hybrid (Y2H) system was adopted to screen for CSFV NS5A interactive proteins in the cDNA library of the swine umbilical vein endothelial cell (SUVEC). Alignment with the NCBI database revealed 16 interactive proteins: DDX5, PSMC3, NAV1, PHF5A, GNB2L1, CSDE1, HSPA8, BRMS1, PPP2R3C, AIP, TMED10, POLR1C, TMEM70, METAP2, CHORDC1 and COPS6. These proteins are mostly related to gene transcription, protein folding, protein degradation and metabolism. The interactions detected by the Y2H system should be considered as preliminary results. Since identifying novel pathways and host targets, which play essential roles during infection, may provide potential targets for therapeutic development. The finding of proteins obtained from the SUVEC cDNA library that interact with the CSFV NS5A protein provide valuable information for better understanding the interactions between this viral protein and the host target proteins.
College of Veterinary Medicine, Northwest A and F University, Yangling 712100, Shaanxi, China.
Chengcheng
C
Zhang
Lei
L
He
Kai
K
Kang
Heng
H
Chen
Lei
L
Xu
Yanming
Y
Zhang
D000818:Animals; D015153:Blotting, Western; D006692:Classical Swine Fever Virus / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D042783:Endothelial Cells / Q000378:metabolism / Q000821:virology; D015723:Gene Library; D018613:Microscopy, Confocal; D025941:Protein Interaction Mapping; D011506:Proteins / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D020798:Two-Hybrid System Techniques; D014471:Umbilical Veins / Q000166:cytology; D017361:Viral Nonstructural Proteins / Q000378:metabolism*
D017931:DNA Primers; D011506:Proteins; D017361:Viral Nonstructural Proteins