Efficient gene targeting in zebrafish mediated by a zebrafish-codon-optimized cas9 and evaluation of off-targeting effect.
Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, College of Life Sciences, Peking University, Beijing 100871, China; Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen 518055, China.
Da
D
Liu
Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, College of Life Sciences, Peking University, Beijing 100871, China.
Zhanxiang
Z
Wang
Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, College of Life Sciences, Peking University, Beijing 100871, China.
An
A
Xiao
Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, College of Life Sciences, Peking University, Beijing 100871, China.
Yutian
Y
Zhang
Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, College of Life Sciences, Peking University, Beijing 100871, China.
Wenyuan
W
Li
Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, College of Life Sciences, Peking University, Beijing 100871, China; Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, CA 90095, USA.
Yao
Y
Zu
Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, CA 90095, USA.
Shaohua
S
Yao
Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen 518055, China; Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, CA 90095, USA.
Shuo
S
Lin
Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, College of Life Sciences, Peking University, Beijing 100871, China. Electronic address: bzhang@pku.edu.cn.
Bo
B
Zhang
D000818:Animals; D001483:Base Sequence; D064113:CRISPR-Cas Systems; D003062:Codon; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D018390:Gene Targeting / Q000379:methods*; D008969:Molecular Sequence Data; D015027:Zebrafish / Q000235:genetics* / Q000378:metabolism
D003062:Codon; D029941:Fish Proteins