Anti-hyperlipidemic Effect of Polyphenol Extract (Seapolynol(™)) and Dieckol Isolated from Ecklonia cava in in vivo and in vitro Models.
The inhibitory effect of polyphenol extracts (Seapolynol(™), SPN) of the marine brown algae Ecklonia cava and dieckol, a major component of SPN, on hyperlipidemia was investigated in ICR mice fed a high-fat diet (HFD) for five weeks. For analysis of the anti-hyperlipidemic effects of SPN and dieckol, these two agents were given orally on a daily basis to HFD-fed mice for four weeks, starting one week after the beginning of HFD feeding. Groups administered with SPN as well as dieckol showed lower body weight gains than the HFD only group. Administration of SPN and dieckol also resulted in a significant reduction of the level of total cholesterol (TCHO), triglyceride (TG), and low-density lipoprotein (LDL) cholesterol in the serum of HFD-fed mice. In Oil Red O staining using 3T3-L1 preadipocytes, it was shown that both SPN and dieckol markedly inhibited lipid accumulation of 3T3-L1 cells. Furthermore, SPN and dieckol (50 μg/mL) significantly inhibited 3-hydroxyl-methyl glutaryl coenzyme A (HMGCoA) reductase activity in vitro. Taken together, these results suggest that polyphenols of Ecklonia cava (SPN) and dieckol reduce body weight gain and fat accumulation in HFD-induced obese mice, and that their hypolipidemic effect is related to the inhibition of adipogenesis of adipocytes and HMGCoA reductase activity.
Department of Microbiology, College of Medicine, Konyang University, Daejeon 302-718, Korea ; Myonggok Institute for Medical Science, College of Medicine, Konyang University, Daejeon 302-718, Korea.
A-Reum
AR
Yeo
Department of Microbiology, College of Medicine, Konyang University, Daejeon 302-718, Korea ; Myonggok Institute for Medical Science, College of Medicine, Konyang University, Daejeon 302-718, Korea.
Junglim
J
Lee
Department of Microbiology, College of Medicine, Konyang University, Daejeon 302-718, Korea ; Myonggok Institute for Medical Science, College of Medicine, Konyang University, Daejeon 302-718, Korea.
In Hwan
IH
Tae
Department of Microbiology, College of Medicine, Konyang University, Daejeon 302-718, Korea ; Myonggok Institute for Medical Science, College of Medicine, Konyang University, Daejeon 302-718, Korea.
Seok-Rae
SR
Park
Department of Pharmaceutics & Biotechnology, Medical Engineering College, Konyang University, Chungnam 320-711, Korea.
Young Ho
YH
Cho
Division of Applied Chemistry and Biotechnology, College of Engineering, Hanbat National University, Daejeon 305-719, Korea.
Bong Ho
BH
Lee
Botamedi Inc., Jeju 690-756, Korea.
Hyeon Cheol
HC
Shin
Botamedi Inc., Jeju 690-756, Korea.
Seong Ho
SH
Kim
Department of Microbiology, College of Medicine, Konyang University, Daejeon 302-718, Korea ; Myonggok Institute for Medical Science, College of Medicine, Konyang University, Daejeon 302-718, Korea.
Yung Choon
YC
Yoo
Ecklonia cava; Seapolynol™; dieckol; hyperlipidemia; polyphenols