Developmental cigarette smoke exposure: hippocampus proteome and metabolome profiles in low birth weight pups.
Exposure to cigarette smoke during development is linked to neurodevelopmental delays and cognitive impairment including impulsivity, attention deficit disorder, and lower IQ. However, brain region specific biomolecular alterations induced by developmental cigarette smoke exposure (CSE) remain largely unexplored. In the current molecular phenotyping study, a mouse model of 'active' developmental CSE (serum cotinine > 50 ng/mL) spanning pre-implantation through third trimester-equivalent brain development (gestational day (GD) 1 through postnatal day (PD) 21) was utilized. Hippocampus tissue collected at the time of cessation of exposure was processed for gel-based proteomic and non-targeted metabolomic profiling with partial least squares-discriminant analysis (PLS-DA) for selection of features of interest. Ingenuity pathway analysis was utilized to identify candidate molecular and metabolic pathways impacted within the hippocampus. CSE impacted glycolysis, oxidative phosphorylation, fatty acid metabolism, and neurodevelopment pathways within the developing hippocampus.
Department of Environmental and Occupational Health Sciences, School of Public Health and Information Sciences, University of Louisville, Louisville, KY, USA; Birth Defects Center, University of Louisville, Louisville, KY, USA. Electronic address: rachel.neal@louisville.edu.
Rachel E
RE
Neal
Department of Environmental and Occupational Health Sciences, School of Public Health and Information Sciences, University of Louisville, Louisville, KY, USA.
Jing
J
Chen
Department of Molecular, Cellular, and Craniofacial Biology, ULSD, University of Louisville, Louisville, KY, USA.
Rekha
R
Jagadapillai
Department of Biostatistics and Bioinformatics, School of Public Health and Information Sciences, University of Louisville, Louisville, KY, USA.
Hyejeong
H
Jang
Department of Environmental and Occupational Health Sciences, School of Public Health and Information Sciences, University of Louisville, Louisville, KY, USA.
Bassam
B
Abomoelak
Department of Biostatistics and Bioinformatics, School of Public Health and Information Sciences, University of Louisville, Louisville, KY, USA; Birth Defects Center, University of Louisville, Louisville, KY, USA.
Guy
G
Brock
Department of Molecular, Cellular, and Craniofacial Biology, ULSD, University of Louisville, Louisville, KY, USA; Birth Defects Center, University of Louisville, Louisville, KY, USA.
Robert M
RM
Greene
Department of Molecular, Cellular, and Craniofacial Biology, ULSD, University of Louisville, Louisville, KY, USA; Birth Defects Center, University of Louisville, Louisville, KY, USA.
M Michele
MM
Pisano
D000818:Animals; D001724:Birth Weight; D005260:Female; D005317:Fetal Growth Retardation / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006019:Glycolysis / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D050356:Lipid Metabolism / Q000187:drug effects; D008297:Male; D018811:Maternal Exposure / Q000009:adverse effects*; D055432:Metabolomics / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D055495:Neurogenesis / Q000187:drug effects; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D010085:Oxidative Phosphorylation / Q000187:drug effects; D040901:Proteomics / Q000379:methods; D011897:Random Allocation; D012906:Smoke / Q000009:adverse effects*
D009419:Nerve Tissue Proteins; D012906:Smoke
Cigarette smoke; Development; Hippocampus; Metabolome; Proteome; Tobacco