[Protective effects of pretreatment with neurotrophin-3 on intrathecal ropivacaine in rats].
OBJECTIVE
To investigate the effect of pretreatment with neurotrophin-3 (NT-3) on intrathecal ropivacaine in rats.


METHODS
A total of 144 male Sprague Dawley rats weighing 280-320 g were successfully implanted with microspinal cather following the improved methods of Yaksh. The rats were randomly divided into 4 groups and given saline (Group NS, n=36), 0.5% ropivacaine (Group M, n=36), 1% ropivacaine (Group R, n=36), and ropivacaine+NT-3 (Group T, n=36). The rats received 0.12 mL/ kg body weight of ropivacaine at 0.5% or 1%, or normal saline only, via an implanted intrathecal catheter at 90-min interval for 12 h in Group NS, M, R and T. In the meantime the rats also received NT-3 0.1 mg/kg in group T. On days 1, 3, 5, 7, 14 and 28, we assessed the paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL), behavioural change and histopathological damage score changed for possible neuronal injury within the spinal cord.


RESULTS
Compared with Group NS and Group M, the PWMT and PWTL were significantly higher on 1, 3, 5 d and the histopathological damage score was significantly higher on 1, 3, 5, 7, 14 d in Group R (P<0.05). Compared with Group T, the PWMT and PWTL in Group R were significantly higher on 1, 3, 5 d and histopathological damage score was significantly higher on 5, 7, 14 d (P<0.05).


CONCLUSIONS
NT-3 pretreatment in mice has obvious protective effect against repeated intrathecal injection of 1% ropivacaine in the spinal nerve.
Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha 410008, China.
Zhihua
Z
Sun
Qulian
Q
Guo
Xiaoping
X
Xu
Zhong
Z
Zhang
Na
N
Wang
Zongbin
Z
Song
D000577:Amides / Q000009:adverse effects*; D000818:Animals; D007278:Injections, Spinal; D008297:Male; D020933:Neurotrophin 3 / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077212:Ropivacaine; D013116:Spinal Cord / Q000187:drug effects*
D000577:Amides; D020933:Neurotrophin 3; D000077212:Ropivacaine