Proteomics approaches for the studies of bone metabolism.
Bone is an active tissue, in which bone formation by osteoblast is followed by bone resorption by osteoclasts, in a repeating cycle. Proteomics approaches may allow the detection of changes in cell signal transduction, and the regulatory mechanism of cell differentiation. LC-MS/MS-based quantitative methods can be used with labeling strategies, such as SILAC, iTRAQ, TMT and enzymatic labeling. When used in combination with specific protein enrichment strategies, quantitative proteomics methods can identify various signaling molecules and modulators, and their interacting proteins in bone metabolism, to elucidate biological functions for the newly identified proteins in the cellular context. In this article, we will briefly review recent major advances in the application of proteomics for bone biology, especially from the aspect of cellular signaling.
Department of Veterinary Biochemistry, BK21 and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
Ji-Hyun
JH
Lee
Department of Veterinary Biochemistry, BK21 and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
Je-Yoel
JY
Cho
D000818:Animals; D001847:Bone Diseases / Q000378:metabolism / Q000628:therapy; D001842:Bone and Bones / Q000378:metabolism*; D006801:Humans; D040901:Proteomics / Q000379:methods*