[Population genetic study of Oncomelania hupensis from three provinces in lower reaches of Yangtze River].
OBJECTIVE
To analyze the population genetic structure and genetic diversity of Oncomelania hupensis population from Jiangxi, Anhui and Jiangsu provinces along the lower reaches of the Yangzte River in order to explore the application values of rDNA-ITS molecular markers in the surveillance of snail dispersal.


METHODS
The samples about 9 species of O. hupensis snails were collected separately from Jiangxi, Anhui and Jiangsu provinces, and their genomes were extracted, then the partial gene of rDNA-ITS was amplified, cloned and sequenced. Next, the population genetic diversity parameters, including Fixation index (F(st)), genetic distance, and genetic variation were calculated. By using the neighbor-joining method, the family map was constructed based on the haplotype.


RESULTS
A total of 78 haplotypes among 93 sequences were acquired from 9 populations. The average haplotype diversity and nucleotide diversity of 9 populations were 0.988 and 0.012 88, respectively, which showed that the genetic diversity level of O. hupensis snail population was high. The variation was found mainly among the samples, the genetic distances between populations were ranged from 0.001 6 to 0.002 3, which showed a higher degree of genetic differentiation among snail populations. The family map showed that all the haploid types could be divided into three major branches.


CONCLUSIONS
The genetic diversity of O. hupensis population in Jiangxi, Anhui and Jiangsu provinces along the lower reaches of the Yangtze River mainly exists among individuals, and there are no obvious genetic differences among populations. The application of rDNA-ITS molecular markers in surveillance of snail dispersal is worth further discussing.
College of Life Science, Shaanxi Normal University, Xi'an 710119, China.
Bin
B
Cui
College of Life Science, Shaanxi Normal University, Xi'an 710119, China.
Zhi-Ping
ZP
Huo
Jiangsu Institute of Parasitic Diseases, China.
Kun
K
Yang
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Key Laboratory of Parasite and Vector Biology, Ministry of Health, WHO Collaborating Center of Malaria, Schistosomiasis and Filariasis, China.
Li
L
Zhang
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Key Laboratory of Parasite and Vector Biology, Ministry of Health, WHO Collaborating Center of Malaria, Schistosomiasis and Filariasis, China.
Pin
P
Yang
College of Life Science, Shaanxi Normal University, Xi'an 710119, China.
Ping
P
You
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Key Laboratory of Parasite and Vector Biology, Ministry of Health, WHO Collaborating Center of Malaria, Schistosomiasis and Filariasis, China.
Shi-Zhu
SZ
Li
D000818:Animals; D002681:China; D014644:Genetic Variation; D005828:Genetics, Population; D006239:Haplotypes; D045483:Rivers; D012908:Snails / Q000235:genetics*