Galangin, a dietary flavonol inhibits tumor initiation during experimental pulmonary tumorigenesis by modulating xenobiotic enzymes and antioxidant status.
The aim of present study was to elucidate anti-initiating efficacy of galangin against benzo(a)pyrene (B(a)P)-induced lung carcinogenesis in male Swiss albino mice. Therefore, the activities of xenobiotic metabolic enzymes such as phase I and II were examined in lung as well as liver tissues (to compare the effects between target and non-target organs). Besides, the activities/levels of tissue marker enzymes, antioxidants, lipid peroxidation (LPO), cytochrome P450 1A1 (CYP1A1) expressions and histological observation of lungs were also analyzed. B(a)P (50 mg/kg body weight) was administered to male Swiss albino mice (20-25 g) to experimentally induce lung cancer. B(a)P-induced animals showed increased activity of phase I (Cytochrome P450, Cytochrome b5, NADPH Cytochrome P450 redcutase and NADH Cytochrome b5 reductase) drug metabolic enzymes, LPO levels, tissue marker enzymes and decreased activity of phase II metabolic enzymes (glutathione-S-transferase, DT-diaphorase and UDP-glucuronyl transferase) as well as antioxidant levels. Histological examination of lungs revealed severe alveolar and bronchiolar damages in B(a)P-induced mice. Immunohistochemical and western blot analysis of CYP1A1 increased significantly in lung tissues of B(a)P-induced animals. Treatment with galangin (20 mg/kg body weight) efficiently counteracted all the above anomalies and restored cellular homeostasis. Our results demonstrate that galangin can modify xenobiotic enzymes in murine model of pulmonary tumorigenesis.
Department of Zoology, University of Madras, Guindy Campus, Chennai, 600 025, Tamilnadu, India.
Dinesh
D
Devadoss
Department of Animal Health and Management, Alagappa University, Karaikudi, 630 003, Tamilnadu, India.
Manikandan
M
Ramar
Department of Zoology, University of Madras, Guindy Campus, Chennai, 600 025, Tamilnadu, India. arulvasu2005@gmail.com.
Arulvasu
A
Chinnasamy
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D063646:Carcinogenesis / Q000187:drug effects* / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology / Q000627:therapeutic use*; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000201:enzymology* / Q000473:pathology; D008297:Male; D051379:Mice; D015262:Xenobiotics / Q000378:metabolism
D000975:Antioxidants; D005419:Flavonoids; D015262:Xenobiotics; C037032:galangin; C041477:3-hydroxyflavone
Benzo(a)pyrene; CYP1A1 and antioxidants; Galangin; Lung cancer; Xenobiotic metabolic enzymes