Quartz crystal microbalance for comparison of calcium phosphate precipitation on planar and rough phospholipid bilayers.
The planar and rough phospholipid bilayers at the surfaces of quartz crystal and titania-modified quartz crystal were fabricated via the surface modification, respectively, and characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), cyclic voltammetry (CV) and piezoelectric measurement. The formation of calcium phosphate on planar and rough phospholipid bilayers was investigated in detail using in situ quartz crystal microbalance (QCM) and X-ray diffraction (XRD) techniques. The obtained results showed that the calcium phosphate precipitation was closely related to the roughness and surface potential of phospholipid bilayers. Compared with planar phospholipid bilayers, the rough phospholipid bilayers exhibited a higher deposition rate of calcium phosphate. The presence of anionic phosphatidylserine (PS) in phosphatidylcholine (PC)/PS phospholipid induced PC/PS surface with negative charge, thus showing significantly enhanced calcium phosphate precipitation.
Institute of Materials Science and Engineering, Henan Polytechnic University, Jiaozuo 454000, China; Cultivating Base for Key Laboratory of Environment-friendly Inorganic Materials in University of Henan Province, Henan Polytechnic University, Jiaozuo 454000, China.
Zhengpeng
Z
Yang
Institute of Materials Science and Engineering, Henan Polytechnic University, Jiaozuo 454000, China; College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China. Electronic address: xchen.2008@yahoo.com.cn.
Chunjing
C
Zhang
Institute of Materials Science and Engineering, Henan Polytechnic University, Jiaozuo 454000, China.
Lina
L
Huang
D002130:Calcium Phosphates / Q000138:chemical synthesis* / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D010316:Particle Size; D010743:Phospholipids / Q000737:chemistry*; D058525:Quartz Crystal Microbalance Techniques; D013499:Surface Properties
D002130:Calcium Phosphates; D008051:Lipid Bilayers; D010743:Phospholipids; C020243:calcium phosphate
Biomaterials; Biomimetic; Calcium phosphate precipitation; Interfaces; Quartz crystal microbalance; Roughness