Contribution to the structural characterization of eucaryotic PSI reaction centre - II. Characterization of a highly purified photoactive SDS-CP1 complex.
Under precise conditions, SDS PAGE† allows purification of a photoactive P700-chla-protein complex from eucaryotic cells. The yield of P700 recovery is close to 100%. A total protein content equivalent to about 140 kD for one mole of P700 has been estimated by chemical analysis, and electrophoresis revealed the presence of two peptidic chains with MWs close to 65 kD. Photochemical and structural properties of this complex are given and compared with those of other complexes previously isolated.
Service de Biochimie, Department de Biologie, C.E.N. de SACLAY, BP No2, Gif-sur-Yvette, 91 190, France.
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Setif
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Acker
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Lagoutte
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Duranton