Anti-hemorrhagic effect of hydro-alcoholic extract of the leaves of Mikania glomerata in lesions induced by Bothrops jararaca venom in rats.
OBJECTIVE
To investigate the effects of hydroalcoholic leaf extract of Mikania glomerata Spreng (Asteraceae) on the activity of Bothrops jararaca snake venom in Wistar rats.


METHODS
Fifty four rats Wistar were divided into six groups of nine animals in each: control treated with saline; control treated with B. jararaca venom; control treated with M. glomerata extract; B. jararaca venom incubated with M. glomerata extract at proportions of 1:1, 1:2, and 1:4.


RESULTS
Histopathological and morphometric analysis showed that intradermal administration of snake venom incubated with the hydroalcoholic extract at proportions of 1:1, 1:2 and 1:4 promoted a significant reduction in the number of inflammatory cells and a marked decrease in edema after the third hour. There was also a significant reduction in the intensity of the hemorrhagic halo in animals receiving the snake venom incubated with the extract, with the observation of a progressive and parallel inhibition with increasing proportion of M. glomerata.


CONCLUSIONS
The Mikania glomerata hydroalcoholic extract exerted effective anti-inflammatory and antihemorrhagic activity against the effects induced by Bothrops jararaca snake venom.
Herminio Ometto University Center, School of Pharmacy, ArarasSP, Brazil.
Vanessa Barreto
VB
Mourão
Herminio Ometto University Center, School of Pharmacy, ArarasSP, Brazil.
Gleice Mara
GM
Giraldi
Herminio Ometto University Center, ArarasSP, Brazil.
Lia Mara Grosso
LM
Neves
Herminio Ometto University Center, ArarasSP, Brazil.
Fernanda Oliveira de Gaspari de
FO
Gaspi
State University of Campinas, Multidisciplinary Center for Chemical, Biological and Agricultural, Phytochemistry Division, CampinasSP, Brazil.
Rodney Alexandre Ferreira
RA
Rodrigues
Herminio Ometto University Center, ArarasSP, Brazil.
Armindo Antonio
AA
Alves
Herminio Ometto University Center, ArarasSP, Brazil.
Marcelo Augusto Marreto
MA
Esquisatto
Herminio Ometto University Center, ArarasSP, Brazil.
Mauricio Ventura
MV
Mazzi
Herminio Ometto University Center, ArarasSP, Brazil.
Fernanda Aparecida Sampaio
FA
Mendonça
Herminio Ometto University Center, ArarasSP, Brazil.
Gláucia Maria Tech dos
GM
Santos
D000818:Animals; D017837:Bothrops; D003435:Crotalid Venoms / Q000037:antagonists & inhibitors* / Q000633:toxicity; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology; D006470:Hemorrhage / Q000139:chemically induced* / Q000188:drug therapy / Q000473:pathology; D008297:Male; D035742:Mikania; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012871:Skin Diseases / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control
D003435:Crotalid Venoms; D010936:Plant Extracts