Establishment of the purity values of carbohydrate certified reference materials using quantitative nuclear magnetic resonance and mass balance approach.
This work described the assignment of purity values to six carbohydrate certified reference materials, including glucose, fructose, galactose, lactose, xylose and sucrose, according to the ISO Guides 34 and 35. The CRMs' purity values were assigned based on the weighted average of quantitative nuclear magnetic resonance method and mass balance approach with high resolution liquid chromatography - evaporative light scattering detection. All the six CRMs with following value amount fractions: glucose (GBW10062) at a certified purity P ± U (k=2) of (0.99 ± 0.005)%; fructose (GBW10063) at (0.99 ± 0.005)%; galactose (GBW10064) at (0.99 ± 0.007)%; lactose (GBW10065) at (0.99 ± 0.008)%; xylose (GBW10066) at (0.99 ± 0.007)% and sucrose (GBW10067) at (0.99 ± 0.008)%, respectively were certified. The homogeneity of the CRMs was determined by an in-house validated liquid chromatographic method. Potential degradation during storage was also investigated and a shelf-life based on this value was established.
Division of Chemistry, National Institute of Metrology, Beijing 100013, China. Electronic address: superfluidcan@hotmail.com.
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D002241:Carbohydrates / Q000737:chemistry / Q000592:standards*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods / Q000592:standards*; D008970:Molecular Weight; D012015:Reference Standards
D002241:Carbohydrates
Carbohydrates; Certified Reference Material (CRM); Mass balance approach; Quantitative Nuclear Magnetic Resonance (QNMR); Uncertainty