Insulin regulates the novel adipokine adipolin/CTRP12: in vivo and ex vivo effects.
There has been intense interest in the adipokines of the C1q complement/TNF-related protein (CTRP) superfamily. Adipolin (CTRP12) has been described as a novel adipokine, abundantly expressed in adipose tissue with insulin-sensitising and anti-inflammatory effects. We wanted to investigate the effects of acute and chronic hyperinsulinaemia on circulating adipolin concentrations (ELISA) via a prolonged insulin-glucose infusion in humans. We also examined the effects of insulin and the insulin sensitiser, rosiglitazone, on adipolin concentrations (western blotting) in human adipose tissue explants. We found that hyperinsulinaemic induction in healthy lean human subjects significantly increased circulating levels of adipolin (P<0.05 and P<0.01). Furthermore, in subcutaneous adipose tissue explants, insulin significantly increased adipolin protein expression and secretion (P<0.05 and P<0.01). This effect was attenuated by the phosphatidylinositol 3-kinase inhibitor, LY294002 (P<0.05). Moreover, the insulin-sensitising peroxisome proliferator-activated receptor γ (PPARγ) agonist, rosiglitazone, significantly increased adipolin protein expression and secretion in subcutaneous adipose tissue explants (P<0.05 and P<0.01). This effect was inhibited by the PPARγ antagonist, GW9662 (P<0.05). Our data provide novel insights into adipolin physiology in human subjects.
Warwick Medical School, University of Warwick, Coventry CV4 7AL, UK Department of Obstetrics and Gynaecology, Birmingham Heartlands Hospital, Heart of England NHS Foundation Trust, Birmingham, UK Department of Endocrinology and Metabolic Diseases, Polish Mother's Memorial Research Institute, The Medical University of Lodz, Lodz, Poland.
Bee K
BK
Tan
Krzysztof C
KC
Lewandowski
Joseph Paul
JP
O'Hare
Harpal S
HS
Randeva
D054392:Adipokines / Q000097:blood / Q000235:genetics*; D000273:Adipose Tissue / Q000378:metabolism; D000328:Adult; D005947:Glucose / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D008297:Male; D015854:Up-Regulation; D055815:Young Adult
D054392:Adipokines; C584654:C1QTNF12 protein, human; D007328:Insulin; D005947:Glucose
PI3K; adipokine; adipolin; insulin; rosiglitazone