Identification and characterization of hydrocolloid from Cordia myxa leaf.
Hot water extraction technique was employed to extract the hydrocolloid from Cordia myxa leaf (PCM). The optimal conditions for extraction of PCM were determined using response surface methodology. A Box-Behnken design (BBD) was applied to evaluate the effects of three independent variables (extraction time (X1: 1-4 h), extraction temperature (X2: 55-95 °C), and water to raw material ratio (X3: 5-30 ml/g) on the extraction yield of PCM. The content of moisture, water-soluble and water-insoluble ash, crude protein and total phenol were determined in the extracted hydrocolloid by standard methods. The maximum hydrocolloid extraction yield (9.501±0.15%) was achieved by using extraction time of 4.94 h, extraction temperature of 94.91 °C and water to raw material ratio of 21.74 ml/g. The contents of moisture, crude protein, water-soluble and water-insoluble ash and total phenol were 21.63±0.94%, 14.27±0.55%, 3.07±0.16% and 2.61±0.19 mg galic acid/g, respectively.
Department of Food Science and Engineering, Faculty of Agricultural Engineering and Technology, University of Tehran, Iran. Electronic address: vsamavati@ut.ac.ir.
Vahid
V
Samavati
Department of Food Science & Technology, Islamic Azad University, Ayatollah Amoli Branch, Amol, Iran.
Mohammad
M
Lorestani
Department of Food Science & Technology, Ramin Agricultural & Natural Resources University, Ahvaz, Iran.
Sajjad
S
Joolazadeh
D005591:Chemical Fractionation / Q000379:methods*; D003102:Colloids; D031213:Cordia / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D017665:Hydroxyl Radical / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D018515:Plant Leaves / Q000737:chemistry*; D010940:Plant Proteins / Q000032:analysis; D013481:Superoxides / Q000737:chemistry; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry
D003102:Colloids; D016166:Free Radical Scavengers; D010936:Plant Extracts; D010940:Plant Proteins; D014867:Water; D013481:Superoxides; D017665:Hydroxyl Radical
Box–Behnken; Hydrocolloid; Identification; Modeling