Ultra structure analysis of cell-cell interactions between pericytes and neutrophils in vitro.
Neutrophils' adhesion to the endothelium during inflammatory is a well-known processes. In contrast the interaction of neutrophils with cells of the neurovascular unit after they have been transmigrated into the brain is less clear. Recently, lymphocyte function-associated antigen-1 (LFA-1) dependent subendothelial crawling of neutrophils has been observed in vivo. This is mediated by intracellular adhesion molecule-1 (ICAM-1), which is expressed on the cell surface of pericytes. In our work we demonstrated in vitro a cell-cell interaction between porcine brain capillary pericytes (PBCPs) and neutrophils, with further characterization of the initial contact between these cells. PBCPs increase ICAM-1 protein expression in response to the cytokine tumor necrosis factor-alpha (TNF-α). Furthermore, an increase in neutrophil adhesion to PBCPs was determined by immunofluorescence staining. By means of scanning force microscopy (SFM), we could additionally show that pericytes as well as neutrophils form cell extensions towards the neighboring cell. Interestingly, these extensions differ for different cell types.
Institute of Biochemistry, Westfälische Wilhelms-Universität Münster, Wilhelm-Klemm-Str. 2, 48149 Münster, Germany.
Christian
C
Pieper
Institute of Biochemistry, Westfälische Wilhelms-Universität Münster, Wilhelm-Klemm-Str. 2, 48149 Münster, Germany. Electronic address: gallah@uni-muenster.de.
Hans-Joachim
HJ
Galla
D000818:Animals; D015153:Blotting, Western; D002448:Cell Adhesion; D002450:Cell Communication; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D018625:Microscopy, Atomic Force; D009504:Neutrophils / Q000166:cytology*; D020286:Pericytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1
Cell–cell adhesion; Neutrophils; Pericytes; Scanning force microscopy (SFM); Ultrastructure