Colorimetric sensor array allows fast detection and simultaneous identification of sepsis-causing bacteria in spiked blood culture.
Sepsis is a medical emergency demanding early diagnosis and tailored antimicrobial therapy. Every hour of delay in initiating effective therapy measurably increases patient mortality. Blood culture is currently the reference standard for detecting bloodstream infection, a multistep process which may take one to several days. Here, we report a novel paradigm for earlier detection and the simultaneous identification of pathogens in spiked blood cultures by means of a metabolomic "fingerprint" of the volatile mixture outgassed by the organisms. The colorimetric sensor array provided significantly faster detection of positive blood cultures than a conventional blood culture system (12.1 h versus 14.9 h, P < 0.001) while allowing for the identification of 18 bacterial species with 91.9% overall accuracy within 2 h of growth detection. The colorimetric sensor array also allowed for discrimination between unrelated strains of methicillin-resistant Staphylococcus aureus, indicating that the metabolomic fingerprint has the potential to track nosocomial transmissions. Altogether, the colorimetric sensor array is a promising tool that offers a new paradigm for diagnosing bloodstream infections.
Specific Technologies, Mountain View, California, USA.
Sung H
SH
Lim
Samantha
S
Mix
Zeyu
Z
Xu
Brian
B
Taba
Indre
I
Budvytiene
Anders N
AN
Berliner
Nuria
N
Queralto
Yair S
YS
Churi
Richard S
RS
Huang
Michael
M
Eiden
Raymond A
RA
Martino
Paul
P
Rhodes
Niaz
N
Banaei
D001419:Bacteria / Q000145:classification / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods*; D015374:Biosensing Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology*; D001774:Blood Chemical Analysis / Q000379:methods*; D003124:Colorimetry / Q000379:methods*; D006801:Humans; D055432:Metabolomics / Q000379:methods; D018805:Sepsis / Q000175:diagnosis* / Q000382:microbiology; D013995:Time