Simultaneous determination of fifteen constituents of jitai tablet using ultra high-performance liquid chromatography coupled with triple quadrupole electrospray tandem mass spectrometry.
An ultra-high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) method was developed for simultaneous determination of fifteen constituents in Jitai tablet (JTT), a complex Traditional Chinese Medicine prescription (TCMP) used in treating opiate addiction. Benefitting from a small particle size (1.8 µm) C18 column, accelerated analysis with satisfactory resolution, sensitivity and selectivity were achieved in a single run within 7 min with linear gradient elution of acetonitrile-0.1% (v/v) formic acid in water. The analytical signal was obtained by multiple reaction monitoring transitions via electrospray ionization source operating in both positive and negative ionization mode. The approach was validated for linearity, sensitivity, precision, repeatability, stability and recovery. All analytes showed good linearity over a wide concentration range (r > 0.99). The method limits ranged from 0.03 ng/mL to 19.35 ng/mL which are sensitive enough for quality control studies. The developed method was successfully applied to the simultaneous determination of fifteen constituents in JTT. In conclusion, our experimental results demonstrate that UHPLC-ESI-MS/MS is a useful approach for the overall quality assessment of complex TCMPs.
School of Pharmacy, Second Military Medical University, Shanghai 200433, China. shupingwang2007@163.com.
Shuping
S
Wang
Department of Pharmacy, Changhai Hospital, Second Military Medical University, Shanghai 200433, China. naizhe168@163.com.
Peng
P
Fu
College of Pharmacy, Nankai University, Tianjin 300071, China. mark_2004@163.com.
Lei
L
Liu
National Engineering Research Center for TCM, Shanghai 201203, China. pharmacy2001@163.com.
Lingling
L
Wang
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China. pypengchengcheng@126.com.
Chengcheng
C
Peng
School of Pharmacy, Second Military Medical University, Shanghai 200433, China. pypengchengcheng@126.com.
Weidong
W
Zhang
School of Pharmacy, Second Military Medical University, Shanghai 200433, China. lyliurh@126.com.
Runhui
R
Liu
D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D006801:Humans; D009293:Opioid-Related Disorders / Q000188:drug therapy* / Q000473:pathology; D021241:Spectrometry, Mass, Electrospray Ionization; D013607:Tablets / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D013607:Tablets