Estrogen-regulated transcription of the uteroglobin gene from the brown hare (Lepus capensis).
To get further insights on the estrogen regulation of the uteroglobin (UG) gene, the 5'-flanking region of the UG gene from the brown hare (Lepus capensis) (Lc) was cloned and compared with those from two phylogenetically related species: the rabbit (Orictolagus cuniculus) (Oc) and the volcano rabbit (Romerolagus diazi) (Rd). The Lc-UG gene is very similar to those from rabbits (94%) and volcano rabbits (95%), and shares a number of genetic elements, including an estrogen response element (ERE). The estrogen-regulated transcription of a series of progressive 5'-deletion mutants of the Lc-UG gene, identified a functional ERE in the promoter region exhibiting the same orientation and relative position than that previously described in rabbits. The Lc-ERE is identical to the Oc-ERE, but different from both the Rd-ERE and the consensus ERE (c-ERE) by one nucleotide. We also detected important species-specific differences in the estrogen-regulated transcription of the UG gene. A luciferase reporter driven by 333 base pairs (bp) of the Lc-UG promoter elicited a higher response to estradiol than its related counterparts when expressed in estrogen-sensitive MCF-7 cells. Several ERE-like motifs which failed to act as functional EREs were also identified; one of them exhibited two mismatches in its palindromic sequence, a characteristic exhibited in many other natural occurring EREs, including the Rd-ERE.
Research Unit in Reproductive Medicine, Hospital de Gineco-Obstetricia No. 4, Dr. Luis Castelazo Ayala, Instituto Mexicano del Seguro Social, Av. Río Magdalena 289 sexto piso, Col. Tizapán San Ángel, C.P. 01090 México, D.F., Mexico.
Adriana
A
Acosta-Montesdeoca
Research Unit in Reproductive Medicine, Hospital de Gineco-Obstetricia No. 4, Dr. Luis Castelazo Ayala, Instituto Mexicano del Seguro Social, Av. Río Magdalena 289 sexto piso, Col. Tizapán San Ángel, C.P. 01090 México, D.F., Mexico.
Teresa
T
Zariñán
Research Unit in Reproductive Medicine, Hospital de Gineco-Obstetricia No. 4, Dr. Luis Castelazo Ayala, Instituto Mexicano del Seguro Social, Av. Río Magdalena 289 sexto piso, Col. Tizapán San Ángel, C.P. 01090 México, D.F., Mexico.
Alfredo
A
Ulloa-Aguirre
Research Unit in Reproductive Medicine, Hospital de Gineco-Obstetricia No. 4, Dr. Luis Castelazo Ayala, Instituto Mexicano del Seguro Social, Av. Río Magdalena 289 sexto piso, Col. Tizapán San Ángel, C.P. 01090 México, D.F., Mexico. Electronic address: ruben.gutierrezs@imss.gob.mx.
Rubén
R
Gutiérrez-Sagal
D024506:5' Flanking Region / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D004958:Estradiol / Q000494:pharmacology; D047628:Estrogen Receptor alpha / Q000378:metabolism; D004967:Estrogens / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D034621:Hares / Q000235:genetics*; D006801:Humans; D061986:MCF-7 Cells; D008969:Molecular Sequence Data; D059372:Nucleotide Motifs / Q000235:genetics; D011817:Rabbits; D020218:Response Elements / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014158:Transcription, Genetic / Q000187:drug effects*; D014598:Uteroglobin / Q000235:genetics* / Q000378:metabolism
D047628:Estrogen Receptor alpha; D004967:Estrogens; D004958:Estradiol; D014598:Uteroglobin
Estradiol; Estrogen response element (ERE); Hare; Lepus capensis; Secretoglobins; Uteroglobin