Multiplex high-resolution melting analysis as a diagnostic tool for detection of plasmid-mediated AmpC β-lactamase genes.
High-resolution melting (HRM) analysis can be a diagnostic tool to evaluate the presence of resistance genes with the added bonus of discriminating sequence modifications. A real-time, multiplex PCR assay using HRM was designed for the detection of plasmid-mediated ampC genes. The specificity and sensitivity of this assay were 96% and 100%, respectively.
Creighton University School of Medicine, Department of Medical Microbiology and Immunology, Center for Research in Anti-Infectives and Biotechnology, Omaha, Nebraska, USA.
Chelsie N
CN
Geyer
Nancy D
ND
Hanson
D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics*; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000235:genetics; D004756:Enterobacteriaceae Infections / Q000382:microbiology; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D010957:Plasmids; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D044366:Transition Temperature; D001618:beta-Lactamases / Q000032:analysis* / Q000235:genetics*
D001426:Bacterial Proteins; C048795:AmpC beta-lactamases; D001618:beta-Lactamases