Supplementation of the diet with Salecan attenuates the symptoms of colitis induced by dextran sulphate sodium in mice.
As a water-soluble extracellular β-glucan produced by Agrobacterium sp. ZX09, Salecan has an excellent toxicological profile and exerts multiple physiological effects. The aims of the present study were to investigate the protective effects of a Salecan diet in the well-defined dextran sulphate sodium (DSS) model of experimental murine colitis and to elucidate the mechanism involved in its effects with special attention being paid to its effect on the production of TNF-α, a primary mediator involved in the inflammatory response. Male C57BL/6J mice were fed a diet supplemented with either 4 or 8 % Salecan for 26 d and DSS was administered to induce acute colitis during the last 5 d of the experimental period. Several clinical and inflammatory parameters as well as mRNA expression of TNF-α and Dectin-1 were evaluated. The results indicated that the dietary incorporation of Salecan attenuated the severity of DSS colitis as evidenced by the decreased disease activity index, reduced severity of anaemia, attenuated changes in colon architecture and reduced colonic myeloperoxidase activity. This protection was associated with the down-regulation of TNF-α mRNA levels, which might derive from its ability to increase Dectin-1 mRNA levels. In conclusion, the present study suggests that Salecan contributes to the reduction of colonic damage and inflammation in mice with DSS-induced colitis and holds promise as a new, effective nutritional supplement in the management of inflammatory bowel disease.
Center for Molecular Metabolism, Nanjing University of Science and Technology, Nanjing 210094, People's Republic of China.
Mengyi
M
Zhou
East Hospital, Tongji University, Shanghai 200120, People's Republic of China.
Zhongqiu
Z
Wang
Center for Molecular Metabolism, Nanjing University of Science and Technology, Nanjing 210094, People's Republic of China.
Jinping
J
Chen
Center for Molecular Metabolism, Nanjing University of Science and Technology, Nanjing 210094, People's Republic of China.
Yibei
Y
Zhan
Center for Molecular Metabolism, Nanjing University of Science and Technology, Nanjing 210094, People's Republic of China.
Tao
T
Wang
Center for Molecular Metabolism, Nanjing University of Science and Technology, Nanjing 210094, People's Republic of China.
Lin
L
Xia
Center for Molecular Metabolism, Nanjing University of Science and Technology, Nanjing 210094, People's Republic of China.
Shiming
S
Wang
State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, People's Republic of China.
Zichun
Z
Hua
Center for Molecular Metabolism, Nanjing University of Science and Technology, Nanjing 210094, People's Republic of China.
Jianfa
J
Zhang
D000704:Analysis of Variance; D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D003106:Colon / Q000378:metabolism* / Q000473:pathology; D016264:Dextran Sulfate; D019587:Dietary Supplements; D004195:Disease Models, Animal; D015536:Down-Regulation; D007249:Inflammation / Q000378:metabolism / Q000517:prevention & control; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D037181:Lectins, C-Type / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009195:Peroxidase / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*; D047071:beta-Glucans / Q000008:administration & dosage*; D000099181:Dectin-1
D016264:Dextran Sulfate; D037181:Lectins, C-Type; D009195:Peroxidase; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D047071:beta-Glucans; D000099181:Dectin-1; C577623:salecan