Structural elucidation of the capsular polysaccharide from Streptococcus pneumoniae serotype 47A by NMR spectroscopy.
The structure of the serotype 47A (Danish nomenclature system) capsular polysaccharide from Streptococcus pneumoniae was elucidated by NMR spectroscopy. The following structure of the repeating heptasaccharide was deduced: [structure: see text]. The serotype 47A capsular polysaccharide is one of 91 structurally and serologically distinct capsular polysaccharides that have been recognized in S. pneumoniae, a significant human pathogenic bacterium and model system in medical microbiology. Structure and NMR spectra are compared to previously solved capsular polysaccharide structures of other serotypes.
Carlsberg Laboratory, 10 Gamle Carlsberg Vej, 1799 Copenhagen V, Denmark. Electronic address: Bent.Petersen@carlsberglab.dk.
Bent O
BO
Petersen
Carlsberg Laboratory, 10 Gamle Carlsberg Vej, 1799 Copenhagen V, Denmark.
Ole
O
Hindsgaul
Farmasøytisk insitutt, Sem Selands vei 3, 0316 Oslo, Norway.
Berit Smestad
BS
Paulsen
Statens Serum Institut, 5 Artillerivej, DK-2300 Copenhagen S, Denmark.
Antonio R
AR
Redondo
Statens Serum Institut, 5 Artillerivej, DK-2300 Copenhagen S, Denmark.
Ian C
IC
Skovsted
D002236:Carbohydrate Conformation; D002473:Cell Wall / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D013296:Streptococcus pneumoniae / Q000737:chemistry*
D011135:Polysaccharides, Bacterial
Capsular polysaccharide; NMR structual assignment; Serotype 47A; Streptococcus pneumoniae