Detection of carbapenemase activities of Bacteroides fragilis strains with matrix-assisted laser desorption ionization--time of flight mass spectrometry (MALDI-TOF MS).
Today resistance against carbapenems is considered an emerging problem in Bacteroides fragilis. Carbapenemase activities produced by aerobic bacteria have been detected by looking at hydrolysis of carbapenems with MALDI-TOF MS, but this technique was never used for anaerobic bacteria. We have developed a protocol for detection and verification of carbapenemase production in B. fragilis within 2.5 h. Twenty-eight strains of B. fragilis were tested. Of the sixteen cfiA-positive strains all showed hydrolysis of ertapenem, whereas the twelve cfiA-negative strains showed no hydrolysis. Ertapenem hydrolysis could be inhibited with 2,6-Pyridinecarboxylic acid (DPA) in all cfiA-positive strains, verifying the presence of the metallo-beta-lactamase. Here we show a rapid way to detect carbapenemase activities of B. fragilis strains.
Department of Clinical Microbiology, Värendsgatan 8, 35185 Växjö, Sweden. Electronic address: asa.c.johansson@ltkronoberg.se.
Asa
A
Johansson
Institute of Clinical Microbiology, Faculty of General Medicine, University of Szeged, Szeged, Hungary.
Elisabeth
E
Nagy
Institute of Clinical Microbiology, Faculty of General Medicine, University of Szeged, Szeged, Hungary.
József
J
Sóki
D001426:Bacterial Proteins / Q000032:analysis*; D001431:Bacteriological Techniques / Q000379:methods*; D001441:Bacteroides fragilis / Q000201:enzymology*; D000077727:Ertapenem; D006801:Humans; D006868:Hydrolysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D001618:beta-Lactamases / Q000032:analysis*; D047090:beta-Lactams / Q000378:metabolism
D001426:Bacterial Proteins; D047090:beta-Lactams; D001618:beta-Lactamases; C063912:carbapenemase; D000077727:Ertapenem
Bacteroides fragilis; Carbapenemase; MALDI-TOF MS