Influence of thin carbonate-containing apatite coating with molecular precursor method to zirconia on osteoblast-like cell response.
The influence of a thin carbonate-containing hydroxyapatite (CA) coating to tetragonal zirconia polycrystal (TZP) on osteoblastlike cell response was investigated. TZP disks were subjected to blasting and acid etching. Thin CA coatings were deposited by the molecular precursor method (TZP-CA). Initial cell adhesion of mouse osteoblast-like cells MC3T3-E1 was enhanced, and marked progress of actin filaments was observed on TZP-CA compared to on TZP. After 3, 5 or 7 days, cell proliferation on TZP-CA was significantly higher than that on TZP. Alkaline phosphatase activity was slightly lower on TZP-CA than on TZP at 7 days, and no difference was observed at 14 or 21 days. At 28 days incubation, collagenous fibers with mineral precipitants accompanied by phosphorous and amino groups were observed. These results indicate that thin CA coating with molecular precursor method offers promise as a means of enhancing cell response, particularly initial adhesion and proliferation of MC3T3-E1 cells.
Division of Oral Implants Research, Oral Health Science Center, Tokyo Dental College.
Hajime
H
Kaneko
Hodaka
H
Sasaki
Shinya
S
Honma
Tohru
T
Hayakawa
Mitsunobu
M
Sato
Yasutomo
Y
Yajima
Masao
M
Yoshinari
D000134:Acid Etching, Dental; D000199:Actins / Q000502:physiology; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001031:Apatites / Q000737:chemistry*; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D010006:Osteoblasts / Q000502:physiology*; D013499:Surface Properties; D015040:Zirconium / Q000737:chemistry*
D000199:Actins; D001031:Apatites; D020099:Coated Materials, Biocompatible; C030782:carboapatite; D015040:Zirconium; D000469:Alkaline Phosphatase; C028541:zirconium oxide