Potential of Ni(II)-NTA-modified poly(ethylene imine) glycopolymers as carrier system for future dendritic cell-based immunotherapy.
Dendritic cells (DCs) play a crucial role in the development of cell-mediated immunotherapy due to their ability to induce and maintain strong immune responses. In our study, we evaluated a biocompatible Ni(II)-NTA-modified poly(ethylene imine) dendritic glycopolymer (Ni(II)-NTA-DG) as new carrier system to increase the antigen uptake into iDCs for future DC-based immunotherapy. Ni(II)-NTA-DG led to an increase in His6-Gp160 uptake in monocytes and iDCs, where His6-Gp160 is localized in the early endosomal and lysosomal compartments. Ni(II)-NTA-DG and the formed polyplexes induced an activation of iDCs, showing an increasing expression of costimulatory molecules CD86, CD80, and proinflammatory cytokines IL-6 and IL-8. Beside no influencing effect of Ni(II)-NTA-DG and polyplexes on the maturation of antigen-bearing DCs, the mature peptide bearing DCs remained their ability to migrate along a gradient of CCR7 ligands. Thus, Ni(II)-NTA-DG with advancing biological properties is a promising carrier system for the future application in DC-based immunotherapy.
Leibniz-Institut für Polymerforschung Dresden e.V. , Hohe Straße 6, D-01069 Dresden, Germany.
N
N
Hauptmann
M
M
Pion
R
R
Wehner
M-Á
MÁ
Muñoz-Fernández
M
M
Schmitz
B
B
Voit
D
D
Appelhans
D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D003713:Dendritic Cells / Q000737:chemistry / Q000166:cytology* / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D007097:Imines / Q000737:chemistry* / Q000276:immunology; D007167:Immunotherapy; D015850:Interleukin-6 / Q000737:chemistry / Q000378:metabolism; D016209:Interleukin-8 / Q000737:chemistry / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism; D009000:Monocytes / Q000166:cytology; D011095:Polyethylenes / Q000737:chemistry*
D007097:Imines; D015850:Interleukin-6; D016209:Interleukin-8; D008070:Lipopolysaccharides; D011095:Polyethylenes; C505405:poly(ethylene imine)