Global proteomic analysis of Chelidonium majus and Corydalis cava (Papaveraceae) extracts revealed similar defense-related protein compositions.
Chelidonium majus and Corydalis cava are phylogenetically closely related (Papaveraceae family). The medicinal and pharmaceutical interest in these plants is based on their synthesis of pharmaceutically important compounds, such as alkaloids, flavonoids, phenolic acids and proteins. C. majus shoot and C. cava tuber extracts have been used in traditional folk medicine to treat many diseases, such as fungal, bacterial and viral infections, liver disorders, fever, post-traumatic, colic, abdominal and menstrual pains and even cancer. This study attempts to perform a global comparative proteomic analysis of pharmacologically important extracts from these two closely related unsequenced plant species to gain insights into the protein basis of these plant organs and to compare their common and specific proteomic compositions. We used a shotgun proteomic approach combined with label-free protein quantitation according to the exponentially modified protein abundance index (emPAI). In total, a mean number of 228 protein identification results were recorded in C. cava tuber extracts and about 1240 in C. majus shoot extracts. Comparative analysis revealed a similar stress and defense-related protein composition of pharmacologically active plant species and showed the presence of different pathogenesis-related and low molecular inducible antimicrobial peptides. These findings could form the basis for further elucidation of the mechanism of the strong pharmacological activities of these medicinal plant extracts.
Department of Molecular Virology, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University in Poznań, Umultowska 89, 61-614 Poznań, Poland. Electronic address: rnawrot@amu.edu.pl.
Robert
R
Nawrot
Max-Planck-Institute of Molecular Plant Physiology, Am Mühlenberg 1, D-14476 Potsdam-Golm, Germany.
Henrik
H
Zauber
Max-Planck-Institute of Molecular Plant Physiology, Am Mühlenberg 1, D-14476 Potsdam-Golm, Germany.
Waltraud X
WX
Schulze
D000470:Alkaloids / Q000737:chemistry; D028281:Chelidonium / Q000737:chemistry* / Q000502:physiology; D031311:Corydalis / Q000737:chemistry* / Q000502:physiology; D005419:Flavonoids / Q000627:therapeutic use; D008519:Medicine, Traditional; D057865:Plant Immunity; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D018520:Plant Shoots / Q000502:physiology; D035281:Plant Tubers / Q000502:physiology; D010946:Plants, Medicinal; D040901:Proteomics; D013312:Stress, Physiological; D053719:Tandem Mass Spectrometry
D000470:Alkaloids; D005419:Flavonoids; D010940:Plant Proteins; C053376:pathogenesis-related proteins, plant
Chelidonium majus; Corydalis cava; Pathogenesis-related proteins (PR); Plant defense; Shotgun proteomics