Analysis of Intracellular Calcium Signaling in Human Embryonic Stem Cells.
Measurement of changes in intracellular calcium concentration is one of the most common and useful tools for studying signal transduction pathways or cellular responses in basic research and drug screening purposes as well. Increasing number of such applications using human pluripotent stem cells and their derivatives requires development of calcium signal measurements for this special cell type. Here we describe a modified protocol for analysis of calcium signaling events in human embryonic stem cells, which can be used for other pluripotent cell types (such as iPSC) or their differentiated offspring as well.
Institute of Molecular Pharmacology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Diószegi 64, 1113, Budapest, Hungary.
Adrienn
A
Péntek
Katalin
K
Pászty
Ágota
Á
Apáti
D001671:Biochemistry / Q000379:methods*; D020013:Calcium Signaling; D000066449:Human Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism; D042541:Intracellular Space / Q000378:metabolism*; D013223:Statistics as Topic