Functional analysis of the C-terminal region of the vacuolar cadmium-transporting rice OsHMA3.
Rice OsHMA3 is a vacuolar cadmium (Cd) transporter belonging to the P1B-ATPase family and has a long (273aa) C-terminal region. We analyzed the function of the region related to Cd using the transgenic Arabidopsis Col-0 ecotype, which is sensitive to Cd. The OsHMA3 variant containing a truncated (58aa) C-terminal region did not confer Cd tolerance, whereas an OsHMA3 variant containing a longer truncated (105aa) C-terminal region conferred Cd tolerance to transgenic Arabidopsis. We conclude that the C-terminal region, particularly the region containing the first 105aa, has an important role in OsHMA3 activity.
Graduate School of Bioresource Sciences, Akita Prefectural University, Kaidoubata-Nishi 241-438, Shimoshinjyo-Nakano, Akita 010-0195, Japan.
Saori
S
Kumagai
Laboratory of Plant Genetics and Breeding, Faculty of Bioresource Sciences, Akita Prefectural University, Kaidoubata-Nishi 241-438, Shimoshinjyo-Nakano, Akita 010-0195, Japan.
Tatsuya
T
Suzuki
Graduate School of Bioresource Sciences, Akita Prefectural University, Kaidoubata-Nishi 241-438, Shimoshinjyo-Nakano, Akita 010-0195, Japan.
Kouichi
K
Tezuka
Laboratory of Plant Genetics and Breeding, Faculty of Bioresource Sciences, Akita Prefectural University, Kaidoubata-Nishi 241-438, Shimoshinjyo-Nakano, Akita 010-0195, Japan.
Namiko
N
Satoh-Nagasawa
Laboratory of Plant Genetics and Breeding, Faculty of Bioresource Sciences, Akita Prefectural University, Kaidoubata-Nishi 241-438, Shimoshinjyo-Nakano, Akita 010-0195, Japan.
Hidekazu
H
Takahashi
Laboratory of Plant Genetics and Breeding, Faculty of Bioresource Sciences, Akita Prefectural University, Kaidoubata-Nishi 241-438, Shimoshinjyo-Nakano, Akita 010-0195, Japan.
Kenji
K
Sakurai
Laboratory of Plant Genetics and Breeding, Faculty of Bioresource Sciences, Akita Prefectural University, Kaidoubata-Nishi 241-438, Shimoshinjyo-Nakano, Akita 010-0195, Japan.
Akio
A
Watanabe
Faculty of Life and Environmental Sciences, University of Tsukuba, Tennoudai 1-1-1, Tsukuba, 305-8572 Ibaraki, Japan.
Tatsuhito
T
Fujimura
Laboratory of Plant Genetics and Breeding, Faculty of Bioresource Sciences, Akita Prefectural University, Kaidoubata-Nishi 241-438, Shimoshinjyo-Nakano, Akita 010-0195, Japan. Electronic address: akagi@akita-pu.ac.jp.
Hiromori
H
Akagi
D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000502:physiology*; D019943:Amino Acid Substitution; D017360:Arabidopsis; D001692:Biological Transport; D002104:Cadmium / Q000378:metabolism*; D027682:Cation Transport Proteins / Q000737:chemistry / Q000502:physiology*; D016297:Mutagenesis, Site-Directed; D019697:Onions; D012275:Oryza / Q000201:enzymology*; D019441:Plant Epidermis / Q000201:enzymology; D010940:Plant Proteins / Q000737:chemistry / Q000502:physiology*; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D014617:Vacuoles / Q000201:enzymology
D027682:Cation Transport Proteins; D010940:Plant Proteins; D002104:Cadmium; D000251:Adenosine Triphosphatases
C-terminal domain; Cadmium; OsHMA3; P(1B)-ATPase (HMA); Rice; Vacuolar sequestration