Differential expression of tyrosine hydroxylase and transporters in the right and left stellate ganglion of socially isolated rats.
Chronic isolation stress of adult rat males acted increasing gene expression of tyrosine hydroxylase (TH) and neuronal norepinephrine transporter (NET) in the right stellate ganglia, while vesicular monoamine transporter 2 (VMAT2) level remained unchanged. The stress decreased protein level of TH, as well as mRNA levels for NET and VMAT2 in the left stellate ganglia, but expressed no effect on protein levels of these two transporters. These results demonstrate asymmetry in noradrenergic genes in the right and left stellate ganglia during stress and provide molecular evidence to help explain the difference in response to the stress.
Institute "VINCA", Department of Molecular Biology and Endocrinology, University of Belgrade, Belgrade, Serbia.
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Jovanovic
Institute "VINCA", Department of Molecular Biology and Endocrinology, University of Belgrade, Belgrade, Serbia. Electronic address: sladj@vinca.rs.
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Spasojevic
Institute "VINCA", Department of Molecular Biology and Endocrinology, University of Belgrade, Belgrade, Serbia.
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Stefanovic
Institute "VINCA", Department of Molecular Biology and Endocrinology, University of Belgrade, Belgrade, Serbia.
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Dronjak
D000704:Analysis of Variance; D000818:Animals; D002908:Chronic Disease; D007839:Functional Laterality / Q000502:physiology; D005786:Gene Expression Regulation; D008297:Male; D050484:Norepinephrine Plasma Membrane Transport Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012934:Social Isolation; D013233:Stellate Ganglion / Q000378:metabolism*; D013315:Stress, Psychological / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*; D050493:Vesicular Monoamine Transport Proteins / Q000378:metabolism*
D050484:Norepinephrine Plasma Membrane Transport Proteins; D012333:RNA, Messenger; C493454:Slc18a2 protein, rat; C493226:Slc6a2 protein, rat; D050493:Vesicular Monoamine Transport Proteins; D014446:Tyrosine 3-Monooxygenase
Chronic stress; Norepinephrine transporter; Stellate ganglia; Tyrosine hydroxylase; Vesicular monoamine transporter 2