Polymorphisms assessment in the promoter region of IL12RB2 in Amazon leprosy patients.
Leprosy displays a wide clinical spectrum that is dependent of the type of immune response. We investigate here whether polymorphisms in the promoter region of the IL12RB2 gene are associated with susceptibility or resistance to clinical forms of leprosy. Nucleotide sequencing of the promoter region of IL12RB2 encompassing SNPs -1035 A/G, -1033 T/C, -1023 A/G, -650 del/G and -464 A/G was performed on DNA samples from 105 leprosy patients and 108 healthy controls. However, none of the SNPs were associated with susceptibility to the disease or any of its clinical forms. Similarly, haplotype analysis did not show any association. The haplotype -1035A/-1033T/-650G/-464A was prevalent, and homozygosity for this haplotype was associated to a lower distribution of CD4(+) T cells (p=0.041). Our data suggest that polymorphisms present in the promoter region of IL12RB2 may not be associated with susceptibility to leprosy or its clinical forms.
Programa de Pós-graduação em Imunologia Básica e Aplicada - PPGIBA, Universidade Federal do Amazonas - UFAM, Manaus, Brazil.
G A V
GA
Silva
Programa de Pós-graduação em Imunologia Básica e Aplicada - PPGIBA, Universidade Federal do Amazonas - UFAM, Manaus, Brazil.
M P
MP
Santos
Programa de Pós-graduação em Imunologia Básica e Aplicada - PPGIBA, Universidade Federal do Amazonas - UFAM, Manaus, Brazil.
I
I
Motta-Passos
Programa de Pós-graduação em Imunologia Básica e Aplicada - PPGIBA, Universidade Federal do Amazonas - UFAM, Manaus, Brazil.
A L
AL
Boechat
Programa de Pós-graduação em Imunologia Básica e Aplicada - PPGIBA, Universidade Federal do Amazonas - UFAM, Manaus, Brazil; Instituto de Ciências Biológicas, Departamento de Parasitologia, UFAM, Manaus, Amazonas, Brazil.
A
A
Malheiro
Programa de Pós-graduação em Imunologia Básica e Aplicada - PPGIBA, Universidade Federal do Amazonas - UFAM, Manaus, Brazil; Fundação de Medicina Tropical Doutor Heitor Vieira Dourado, Manaus, Amazonas, Brazil; Universidade Nilton Lins, Manaus, Amazonas, Brazil.
R
R
Ramasawmy
Programa de Pós-graduação em Imunologia Básica e Aplicada - PPGIBA, Universidade Federal do Amazonas - UFAM, Manaus, Brazil; Instituto Leônidas e Maria Deane, Fiocruz Amazônia, Manaus, Amazonas, Brazil.
F G
FG
Naveca
Programa de Pós-graduação em Imunologia Básica e Aplicada - PPGIBA, Universidade Federal do Amazonas - UFAM, Manaus, Brazil; Instituto de Ciências Biológicas, Departamento de Morfologia, UFAM, Manaus, Amazonas, Brazil; Universidade Federal de Goiás, Campus Avançado de Catalão, Departamento de Ciências Biológicas, Brazil. Electronic address: lpaula.bio@hotmail.com.
L
L
de Paula
D000293:Adolescent; D000328:Adult; D000368:Aged; D001938:Brazil; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000473:pathology; D016022:Case-Control Studies; D004198:Disease Susceptibility; D005260:Female; D005787:Gene Frequency; D006239:Haplotypes; D006720:Homozygote; D006801:Humans; D007918:Leprosy / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D053707:Receptors, Interleukin-12 / Q000235:genetics*
C508439:IL12RB2 protein, human; D053707:Receptors, Interleukin-12