Effect of photodynamic therapy supplemented with quercetin in HEp-2 cells.
Photodynamic therapy (PDT) is a technique that can be used as a complementary therapy in cancer treatment combined with other therapeutic modalities. Quercetin (QCT) is known to be effective in the treatment of cancer, by reducing the cell viability of different cancer cell lines. This study aimed to evaluate the influence of different concentrations of QCT in PDT on the viability, mitochondrial membrane potential and induction of apoptosis/necrosis in the human larynx carcinoma cells (HEp-2). The HEp-2 cells were treated with aluminum phthalocyanine tetrasulfonate (AlPcS4) and QCT and subsequently irradiated with a diode laser light (685 nm, 35 mW, 4.5 J/cm(2)). The results demonstrated that treatment of HEp-2 cells with high concentrations of QCT (at least 50 μM) reduced cell viability. This response was enhanced in cells subjected to PDT supplemented with high concentrations of QCT. In addition, was observed decrease in the mitochondrial membrane potential and characteristics of late apoptosis and/or initial necrosis process. QCT at concentrations from 50 μM improves PDT-induced cytotoxicity by significantly reducing cell viability by apoptosis and/or necrosis, and mitochondrial membrane potential of Hep-2 cells.
Laboratory of Tissue and Cell Biology, Development and Research Institute, Universidade do Vale do Paraíba (UNIVAP), Av. Shishima Hifumi 2911, Urbanova, 12244-000, São José dos Campos, SP, Brazil.
Rafael
R
de Paula Rodrigues
Italo Rigotti Pereira
IR
Tini
Cristina Pacheco
CP
Soares
Newton Soares
NS
da Silva
D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D007211:Indoles / Q000494:pharmacology; D007822:Laryngeal Neoplasms / Q000188:drug therapy*; D007830:Larynx / Q000473:pathology; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D009336:Necrosis / Q000139:chemically induced; D009942:Organometallic Compounds / Q000494:pharmacology; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology; D011794:Quercetin / Q000494:pharmacology*
D000970:Antineoplastic Agents; D005456:Fluorescent Dyes; D007211:Indoles; D009942:Organometallic Compounds; D017319:Photosensitizing Agents; C048577:aluminum tetrasulfophthalocyanine; D011794:Quercetin
AlPcS4; cell viability; photodynamic therapy; quercetin