Disrupting androgen production of Leydig cells by resveratrol via direct inhibition of human and rat 3β-hydroxysteroid dehydrogenase.
Resveratrol is a polyphenol produced by several plants. It has been demonstrated that it has anti-inflammatory, antitumor, and anti-diabetic effects in animal models. However, its side effects are generally unclear. In the present study, we reported that resveratrol inhibited luteinizing hormone-stimulated androgen production in rat immature Leydig cells. Further analysis demonstrated that it was a competitive inhibitor of rat and human 3β-hydroxysteroid dehydrogenase with IC₆₀ values of 3.87 ± 0.06 and 8.48 ± 0.04 μM, respectively. The inhibition on 3β-hydroxysteroid dehydrogenase was specific since it did not inhibit another hydroxysteroid dehydrogenase 17β-hydroxysteroid dehydrogenase 3 at the highest concentration (100 μM) tested. In conclusion, resveratrol potentially interferes with androgen biosynthesis of rat Leydig cells.
Department of Pharmacy, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.
Ling
L
Li
The 2nd Affiliated Hospital & Research Academy of Reproductive Biomedicine, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
Xiaomin
X
Chen
The 2nd Affiliated Hospital & Research Academy of Reproductive Biomedicine, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
Qiqi
Q
Zhu
The 2nd Affiliated Hospital & Research Academy of Reproductive Biomedicine, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
Dongxin
D
Chen
Department of Anesthesiology & Zhejiang Province Key Laboratory of Anesthesiology, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
Jingjing
J
Guo
The 2nd Affiliated Hospital & Research Academy of Reproductive Biomedicine, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
Wenwen
W
Yao
The 2nd Affiliated Hospital & Research Academy of Reproductive Biomedicine, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
Yaoyao
Y
Dong
The 2nd Affiliated Hospital & Research Academy of Reproductive Biomedicine, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
Jia
J
Wei
Department of Anesthesiology & Zhejiang Province Key Laboratory of Anesthesiology, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
Qingquan
Q
Lian
The 2nd Affiliated Hospital & Research Academy of Reproductive Biomedicine, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China. Electronic address: r_ge@yahoo.com.
Ren-Shan
RS
Ge
Department of Pharmacy, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China. Electronic address: yuanbo1376@163.com.
Bo
B
Yuan
D015096:3-Hydroxysteroid Dehydrogenases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D052244:Endocrine Disruptors / Q000633:toxicity*; D004791:Enzyme Inhibitors / Q000633:toxicity*; D006801:Humans; D007985:Leydig Cells / Q000187:drug effects* / Q000201:enzymology; D007986:Luteinizing Hormone / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D013267:Stilbenes / Q000633:toxicity*; D013739:Testosterone / Q000096:biosynthesis* / Q000097:blood
D052244:Endocrine Disruptors; D004791:Enzyme Inhibitors; D013267:Stilbenes; D013739:Testosterone; D007986:Luteinizing Hormone; D015096:3-Hydroxysteroid Dehydrogenases; D000077185:Resveratrol
3β-Hydroxysteroid dehydrogenase; Rat Leydig cells; Resveratrol; Steroidogenesis; Steroidogenic enzymes