Chemical tumor-targeting of nanoparticles based on metabolic glycoengineering and click chemistry.
Tumor-targeting strategies for nanoparticles have been predominantly based on optimization of physical properties or conjugation with biological ligands. However, their tumor-targeting abilities remain limited and insufficient. Furthermore, traditional biological binding molecules have intrinsic limitations originating from the limited amount of cellular receptors and the heterogeneity of tumor cells. Our two-step in vivo tumor-targeting strategy for nanoparticles is based on metabolic glycoengineering and click chemistry. First, an intravenous injection of precursor-loaded glycol chitosan nanoparticles generates azide groups on tumor tissue specifically by the enhanced permeation and retention (EPR) effect followed by metabolic glycoengineering. These 'receptor-like' chemical groups then enhance the tumor-targeting ability of drug-containing nanoparticles by copper-free click chemistry in vivo during a second intravenous injection. The advantage of this protocol over traditional binding molecules is that there are significantly more binding molecules on the surface of most tumor cells regardless of cell type. The subsequent enhanced tumor-targeting ability can significantly enhance the cancer therapeutic efficacy in animal studies.
Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology , 39-1 Hawolgok-dong, Seongbuk-gu, Seoul 136-791, Republic of Korea .
Sangmin
S
Lee
Heebeom
H
Koo
Jin Hee
JH
Na
Seung Jin
SJ
Han
Hyun Su
HS
Min
So Jin
SJ
Lee
Sun Hwa
SH
Kim
Seok Hyun
SH
Yun
Seo Young
SY
Jeong
Ick Chan
IC
Kwon
Kuiwon
K
Choi
Kwangmeyung
K
Kim
D000818:Animals; D001386:Azides / Q000737:chemistry; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry; D057930:Click Chemistry; D006595:Hexosamines / Q000737:chemistry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D058990:Molecular Targeted Therapy / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism* / Q000627:therapeutic use
D001386:Azides; D006595:Hexosamines; C008322:mannosamine; D048271:Chitosan