LeMAPK1, LeMAPK2, and LeMAPK3 are associated with nitric oxide-induced defense response against Botrytis cinerea in the Lycopersicon esculentum fruit.
Nitric oxide (NO) and mitogen-activated protein kinases (MAPKs) are signal molecules involved in the disease resistance of plants. To investigate the role of tomato MAPKs in the NO-mediated defense response, mature green tomatoes (Lycopersicon esculentum Mill. cv. Qian-xi) were treated with a MAPKs inhibitor (1,4-diamino-2,3-dicyano-1,4-bis(o-amino-phenylmercapto) butadiene (U0126)), NO donor sodium nitroprusside (SNP), and SNP plus U0126. Treatment with U0126 increased the incidence of disease and size of lesion areas in the tomato fruits after being inoculated with Botrytis cinerea. NO enhanced the resistance of the tomato fruits against Botrytis cinerea invasion and the activities of nitric oxide synthase, Chitinase, β-1,3-glucanase, polyphenol oxidase, and phenylalanine ammonia-lyase. However, the effects of NO on disease resistance were weakened by the MAPKs inhibitor. Meanwhile, the relative expression of LeMAPK1, LeMAPK2, and LeMAPK3 in the (SNP + U0126)-treated fruits was lower than that in the SNP-treated fruits. The results suggest that LeMAPK1/2/3 are involved in NO-induced disease resistance of tomato fruits against Botrytis cinerea.
College of Food Science and Nutritional Engineering, China Agricultural University , Beijing, 100083, China.
Yanyan
Y
Zheng
Hui
H
Hong
Lin
L
Chen
Jingyuan
J
Li
Jiping
J
Sheng
Lin
L
Shen
D020171:Botrytis; D002070:Butadienes / Q000494:pharmacology; D060467:Disease Resistance / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005638:Fruit / Q000201:enzymology*; D018551:Solanum lycopersicum / Q000382:microbiology*; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000502:physiology*; D009569:Nitric Oxide / Q000502:physiology*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009570:Nitriles / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D010935:Plant Diseases / Q000382:microbiology*; D010940:Plant Proteins / Q000502:physiology
D002070:Butadienes; D004791:Enzyme Inhibitors; D020030:Nitric Oxide Donors; D009570:Nitriles; D010940:Plant Proteins; C113580:U 0126; D009599:Nitroprusside; D009569:Nitric Oxide; D020928:Mitogen-Activated Protein Kinases