Biochemical phenotype of a common disease-causing mutation and a possible therapeutic approach for the phosphomannomutase 2-associated disorder of glycosylation.
Phosphomannomutase 2 (PMM2) deficiency represents the most frequent type of congenital disorders of glycosylation. For this disease there is no cure at present. The complete loss of phosphomannomutase activity is probably not compatible with life and people affected carry at least one allele with residual activity. We characterized wild-type PMM2 and its most common hypomorphic mutant, p.F119L, which is associated with a severe phenotype of the disease. We demonstrated that active species is the dimeric enzyme and that the mutation weakens the quaternary structure and, at the same time, affects the activity and the stability of the enzyme. We demonstrated that ligand binding stabilizes both proteins, wild-type and F119L-PMM2, and promotes subunit association in vitro. The strongest effects are observed with glucose-1,6-bisphosphate (Glc-1,6-P2) or with monophosphate glucose in the presence of vanadate. This finding offers a new approach for the treatment of PMM2 deficiency. We propose to enhance Glc-1,6-P2 concentration either acting on the metabolic pathways that control its synthesis and degradation or exploiting prodrugs that are able to cross membranes.
Istituto di Chimica Biomolecolare - CNR Pozzuoli, Italy.
Giuseppina
G
Andreotti
Istituto di Biostrutture e Bioimmagini - CNR Napoli, Italy.
Emilia
E
Pedone
Istituto di Chimica Biomolecolare - CNR Pozzuoli, Italy.
Assunta
A
Giordano
Istituto di Biostrutture e Bioimmagini - CNR Napoli, Italy ; Dipartimento di Biologia, Universita' Federico II Napoli, Italy.
Maria Vittoria
MV
Cubellis
Congenital disorders of glycosylation; N-glycosylation; glucose 1,6-bisphosphate; phosphomannomutase