Binding of Y-P30 to syndecan 2/3 regulates the nuclear localization of CASK.
The survival promoting peptide Y-P30 has documented neuroprotective effects as well as cell survival and neurite outgrowth promoting activity in vitro and in vivo. Previous work has shown that multimerization of the peptide with pleiotrophin (PTN) and subsequent binding to syndecan (SDC) -2 and -3 is involved in its neuritogenic effects. In this study we show that Y-P30 application regulates the nuclear localization of the SDC binding partner Calcium/calmodulin-dependent serine kinase (CASK) in neuronal primary cultures during development. In early development at day in vitro (DIV) 8 when mainly SDC-3 is expressed supplementation of the culture medium with Y-P30 reduces nuclear CASK levels whereas it has the opposite effect at DIV 18 when SDC-2 is the dominant isoform. In the nucleus CASK regulates gene expression via its association with the T-box transcription factor T-brain-1 (Tbr-1) and we indeed found that gene expression of downstream targets of this complex, like the GluN2B NMDA-receptor, exhibits a corresponding down- or up-regulation at the mRNA level. The differential effect of Y-P30 on the nuclear localization of CASK correlates with its ability to induce shedding of the ectodomain of SDC-2 but not -3. shRNA knockdown of SDC-2 at DIV 18 and SDC-3 at DIV 8 completely abolished the effect of Y-P30 supplementation on nuclear CASK levels. During early development a protein knockdown of SDC-3 also attenuated the effect of Y-P30 on axon outgrowth. Taken together these data suggest that Y-P30 can control the nuclear localization of CASK in a SDC-dependent manner.
Research Group Neuroplasticity, Leibniz Institute for Neurobiology, Magdeburg, Germany.
Peter
P
Landgraf
Research Group Neuroplasticity, Leibniz Institute for Neurobiology, Magdeburg, Germany.
Marina
M
Mikhaylova
Research Group Neuroplasticity, Leibniz Institute for Neurobiology, Magdeburg, Germany.
Tamar
T
Macharadze
Research Group Neuroplasticity, Leibniz Institute for Neurobiology, Magdeburg, Germany.
Corinna
C
Borutzki
Department of Experimental Obstetrics and Gynaecology, Medical Faculty, Otto-von-Guericke University, Magdeburg, Germany.
Ana-Claudia
AC
Zenclussen
Developmental Neurobiology, Faculty of Biology and Biotechnology, Ruhr University, Bochum, Germany.
Petra
P
Wahle
Research Group Neuroplasticity, Leibniz Institute for Neurobiology, Magdeburg, Germany.
Michael R
MR
Kreutz
D000818:Animals; D015153:Blotting, Western; D019556:COS Cells; D002467:Cell Nucleus / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D015870:Gene Expression / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D051528:Guanylate Kinases / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010455:Peptides / Q000378:metabolism* / Q000494:pharmacology; D011485:Protein Binding; D034622:RNA Interference; D051381:Rats; D020318:Rats, Long-Evans; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053669:Syndecan-2 / Q000235:genetics / Q000378:metabolism*; D053670:Syndecan-3 / Q000235:genetics / Q000378:metabolism*; D020825:T-Box Domain Proteins / Q000378:metabolism; D013997:Time Factors
C121001:NR2B NMDA receptor; D009419:Nerve Tissue Proteins; D010455:Peptides; D016194:Receptors, N-Methyl-D-Aspartate; D053670:Syndecan-3; D020825:T-Box Domain Proteins; C540454:Tbr1 protein, rat; C442243:dermcidin; D049452:Green Fluorescent Proteins; D053669:Syndecan-2; C106868:CASK kinases; D051528:Guanylate Kinases