Investigation into the radiobiological consequences of pre-treatment verification imaging with megavoltage X-rays in radiotherapy.
OBJECTIVE
The aim of this study was to investigate the effect of pre-treatment verification imaging with megavoltage X-rays on cancer and normal cell survival in vitro and to compare the findings with theoretically modelled data. Since the dose received from pre-treatment imaging can be significant, the incorporation of this dose at the planning stage of treatment has been suggested.


METHODS
The impact of imaging dose incorporation on cell survival was investigated by clonogenic assay of irradiated DU-145 prostate cancer, H460 non-small-cell lung cancer and AGO-1522b normal tissue fibroblast cells. Clinically relevant imaging-to-treatment times of 7.5 and 15 min were chosen for this study. The theoretical magnitude of the loss of radiobiological efficacy due to sublethal damage repair was investigated using the Lea-Catcheside dose protraction factor model.


RESULTS
For the cell lines investigated, the experimental data showed that imaging dose incorporation had no significant impact on cell survival. These findings were in close agreement with theoretical results.


CONCLUSIONS
For the conditions investigated, the results suggest that allowance for the imaging dose at the planning stage of treatment should not adversely affect treatment efficacy.


CONCLUSIONS
There is a paucity of data in the literature on imaging effects in radiotherapy. This article presents a systematic study of imaging dose effects on cancer and normal cell survival, providing both theoretical and experimental evidence for clinically relevant imaging doses and imaging-to-treatment times. The data provide a firm foundation for further study into this highly relevant area of research.
Radiotherapy Physics, Northern Ireland Cancer Centre, Belfast Health and Social Care Trust, UK.
W B
WB
Hyland
S J
SJ
McMahon
K T
KT
Butterworth
A J
AJ
Cole
R B
RB
King
K M
KM
Redmond
K M
KM
Prise
A R
AR
Hounsell
C K
CK
McGarry
D002289:Carcinoma, Non-Small-Cell Lung / Q000532:radiotherapy; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008175:Lung Neoplasms / Q000532:radiotherapy; D008297:Male; D008954:Models, Biological; D008962:Models, Theoretical; D009369:Neoplasms / Q000532:radiotherapy*; D011471:Prostatic Neoplasms / Q000532:radiotherapy; D011879:Radiotherapy Dosage; D011881:Radiotherapy, Computer-Assisted / Q000379:methods; D011882:Radiotherapy, High-Energy; D013997:Time Factors