Characterisation of the genetic effects of the ADFP gene and its association with production traits in dairy goats.
Adipose differentiation-related protein (ADFP) is important for regulation of lipid metabolism and insulin secretion in beta-cells. In this study, we investigated polymorphisms within the caprine ADFP gene and determined its relationship with production traits. As there was no sequence information available for the caprine ADFP gene, we generated DNA sequence data and examined the genomic organisation. The caprine ADFP gene is organised into 7 exons and 6 introns that span approximately 8.7 kbp and is transcribed into mRNA containing 1,353 bp of sequence coding for a protein of 450 amino acids. The protein sequences showed substantial similarity (71-99%) to orthologues from cattle, human and mouse. We identified polymorphisms in the sequences using DNA sequencing, PCR-RFLP and forced PCR-RFLP methods. Seven single nucleotide polymorphisms (SNPs) were identified using samples from 4 different goat populations consisting of 1408 healthy and unrelated individuals. Six haplotypes involving the 7 SNPs from the caprine ADFP gene were identified and their effects on production traits were analysed. Haplotype 6 had the highest haplotype frequency and was highly significantly associated with chest circumference and milk yield in the analysed populations. The results of this study suggest that the ADFP gene is a strong candidate gene affecting production traits and may be used for marker-assisted selection and management in Chinese dairy goat breeding programmes.
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 45002, China.
Zhuan-Jian
ZJ
Li
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
Wen-Jiao
WJ
Guo
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 45002, China.
Ya-Dong
YD
Tian
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 45002, China.
Rui-Li
RL
Han
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
Yu-Jia
YJ
Sun
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
Jing
J
Xue
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
Xian-Yong
XY
Lan
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China. Electronic address: chenhong1212@263.net.
Hong
H
Chen
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001947:Breeding; D002417:Cattle; D002681:China; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005091:Exons; D005260:Female; D006041:Goats / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D006239:Haplotypes; D006801:Humans; D007438:Introns; D007774:Lactation; D015810:Linkage Disequilibrium; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology; D051379:Mice; D008892:Milk / Q000378:metabolism; D008969:Molecular Sequence Data; D000070780:Perilipin-2; D020641:Polymorphism, Single Nucleotide; D017386:Sequence Homology, Amino Acid
D008565:Membrane Proteins; C000602982:PLIN2 protein, human; D000070780:Perilipin-2; C000602983:Plin2 protein, mouse; D004247:DNA
ADFP gene; Dairy goat; Molecular characterisation; Production traits; Single nucleotide polymorphism