Tooth bleaching induces changes in the vascular permeability of rat incisor pulps.
OBJECTIVE
To evaluate the inflammatory response in dental pulps of rat incisors subjected to tooth bleaching protocols with different HP concentrations and application times.


METHODS
42 incisors from Wistar rats were submitted to tooth bleaching using concentrations of 25% or 35% HP for treatment times of 15, 30 or 45 minutes. Four non-bleached teeth were used as controls. The animals received an intravenous injection of India ink immediately after the bleaching procedure and were sacrificed 1 hour later. Six bleached teeth from each group and three controls were made transparent, and one sample from each group was processed for histological analysis. The data were statistically analyzed using Kruskal Wallis and Dunn's tests (P < 0.05).


RESULTS
The amount of dental pulp ink content was significantly higher in the samples that were bleached with 35% HP for 30 minutes and with both HP concentrations (25 and 35%) for 45 minutes than in the controls. For the samples bleached with the same HP concentration, the ink content was higher in samples that were bleached for 45 minutes. These results indicate that HP tooth bleaching can induce an increase in vascular permeability in rat incisors. Importantly, this increase is more dependent on the length of the bleaching procedure than on the concentration of the bleaching agent.
Vanessa Guarino
VG
Ferreira
Department of Restorative Dentistry, School of Dentistry, University of São Paulo, SP, Brazil.
Cleber Keiti
CK
Nabeshima
Department of Restorative Dentistry, School of Dentistry, University of São Paulo, SP, Brazil.
Márcia Martins
MM
Marques
Department of Stomatology (Oral Pathology), School of Dentistry, University of São Paulo, SP, Brazil.
Adriana Fraga Costa Samos
AF
Paris
Department of Surgery, School of Veterinary Medicine, University of São Paulo, SP, Brazil.
Marco Antônio
MA
Gioso
Department of Restorative Dentistry, School of Dentistry, Pontifical Catholic University of Rio de Janeiro, Rio de Janeiro, Brazil.
Rodrigo Sant'anna Aguiar
RS
dos Reis
Department of Restorative Dentistry, School of Dentistry, University of São Paulo, SP, Brazil.
Manoel Eduardo de Lima
ME
Machado
D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects*; D002244:Carbon; D004396:Coloring Agents; D003782:Dental Pulp / Q000098:blood supply* / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000008:administration & dosage / Q000627:therapeutic use; D007180:Incisor / Q000187:drug effects*; D008297:Male; D011671:Pulpitis / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014073:Tooth Bleaching / Q000379:methods*; D058205:Tooth Bleaching Agents / Q000008:administration & dosage / Q000627:therapeutic use*
D004396:Coloring Agents; D058205:Tooth Bleaching Agents; C028433:chinese ink; D002244:Carbon; D006861:Hydrogen Peroxide