[Effects of electro-acupuncture at Zusanli point on gastric and intestinal blood flow in rats with acute necrotizing pancreatitis].
OBJECTIVE
To investigate the effects of electro-acupuncture (EA) at Zusanli point on gastric and intestinal blood flow and serum endothelin-1(ET-1), nitricoxide(NO), thromboxaneB2 (TXB2), 6-keto-prostaglandin F1alpha (6-K-PGF1alpha) in rats with acute necrotizing pancreatitis (ANP).


METHODS
Thirty-six male Sprague Dawley (SD) rats were randomly divided into sham operation (sham), ANP and EA groups (n = 12). ANP model was induced by retrograde injection of 5% sodium taurocholate into pancreaticobiliary. EA was applied to Zusanli for 30 min at 2 h and 6 h after the operation in EA group. Gastric and intestinal mucosal blood flow was measured by laser doppler flowmetry (LDF) at 12 h and 24 h after operation, while the levels of serum ET-1, NO, TXB2 and 6-K-PGF1alpha were detected.


RESULTS
The gastric and intestinal blood flow in ANP rats were significantly lower than those in the sham group (P < 0.05), but those of EA groups were higher than those in ANP groups (P < 0.05). The serum ET-1, NO and ET-1/NO levels in ANP group were increased when compared with SO group (P < 0.05). After the electro-acupuncture treatment at Zusanli point, the levels of ET-1, NO and ET-1/N were decreased, and there were significant differences of ET-1 (at 12 h, 24 h), NO (at 12 h) and ET-1/NO (at 24 h) between EA and ANP group (P < 0.05). The levels of serum TXB2, 6-K-PGF1alpha and TXB2/6-K-PGF1alpha in ANP group were also increased (P < 0.05), but those in EA group were decreased, and there were significant differences of TXB2 (at 12 h, 24 h), 6-K-PGF1alpha (at 12 h) and TXB2/6-K-PGF1alpha (at 24 h) compared with ANP groups (P < 0.05).


CONCLUSIONS
Electro-acupuncture at Zusanli point can significantly improve the gastric and intestinal mucosa blood flow in ANP rats, which may be related to the regulation of serum ET-1, NO, TXB2, 6-K-PGF1alpha.
Department of Integrated Traditional Chinese Medcine and Western Medcine, West China Hospital, Sichuan University, Chengdu 610041, China.
Lu
L
Bai
Department of Integrated Traditional Chinese Medcine and Western Medcine, West China Hospital, Sichuan University, Chengdu 610041, China.
Lin
L
Zhu
Department of Integrated Traditional Chinese Medcine and Western Medcine, West China Hospital, Sichuan University, Chengdu 610041, China.
Wen-Fu
WF
Tang
Department of Integrated Traditional Chinese Medcine and Western Medcine, West China Hospital, Sichuan University, Chengdu 610041, China.
Mei-Hua
MH
Wan
Department of Integrated Traditional Chinese Medcine and Western Medcine, West China Hospital, Sichuan University, Chengdu 610041, China.
Wei
W
Huang
Department of Integrated Traditional Chinese Medcine and Western Medcine, West China Hospital, Sichuan University, Chengdu 610041, China.
Guang-Yuan
GY
Chen
D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood; D015669:Acupuncture Points; D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology*; D015671:Electroacupuncture; D019332:Endothelin-1 / Q000097:blood; D005753:Gastric Mucosa / Q000098:blood supply; D041981:Gastrointestinal Tract / Q000098:blood supply*; D007413:Intestinal Mucosa / Q000098:blood supply; D008297:Male; D009569:Nitric Oxide / Q000097:blood; D019283:Pancreatitis, Acute Necrotizing / Q000503:physiopathology* / Q000628:therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013929:Thromboxane B2 / Q000097:blood
D019332:Endothelin-1; D009569:Nitric Oxide; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha