Direct analysis of sialylated or sulfated glycosphingolipids and other polar and neutral lipids using TLC-MS interfaces.
Gangliosides and sulfatides (STs) are acidic glycosphingolipids (GSLs) that have one or more sialic acids or sulfate substituents, in addition to neutral sugars, attached to the C-1 hydroxyl group of the ceramide long chain base. TLC is a widely employed and convenient technique for separation and characterization of GSLs. When TLC is directly coupled to MS, it provides both the molecular mass and structural information without further purification. Here, after development of the TLC plates, the structural analyses of acidic GSLs, including gangliosides and STs, were investigated using the liquid extraction surface analysis (LESA™) and CAMAG TLC-MS interfaces coupled to an ESI QSTAR Pulsar i quadrupole orthogonal TOF mass spectrometer. Coupling TLC with ESI-MS allowed the acquisition of high resolution mass spectra of the acidic GSLs with high sensitivity and mass accuracy, without the loss of sialic acid residues that frequently occurs during low-pressure MALDI MS. These systems were then applied to the analysis of total lipid extracts from bovine brain. This allowed profiling of many different lipid classes, not only gangliosides and STs, but also SMs, neutral GSLs, and phospholipids.
Mass Spectrometry Resource and Department of Biochemistry, Boston University School of Medicine, Boston, MA 02118.
Hyejung
H
Park
Ying
Y
Zhou
Catherine E
CE
Costello
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002855:Chromatography, Thin Layer; D006017:Glycolipids / Q000737:chemistry*; D006028:Glycosphingolipids / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012794:Sialic Acids / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
D006017:Glycolipids; D006028:Glycosphingolipids; D012794:Sialic Acids; C011117:sulfoglycolipids
collision-induced dissociation; gangliosides; phospholipids; sphingomyelins; sulfatides; tandem mass spectrometry; thin-layer chromatography