Whole-mount in situ hybridization on murine skeletogenic tissues.
Whole-mount in situ hybridization (WISH) is a highly sensitive method for spatial and temporal visualization of RNA transcripts throughout an entire tissue. This method is an excellent tool for studying both embryonic development and disease. It is based on sequence complementation between a Digoxigenin (DIG)-labeled antisense RNA probe and the sense RNA transcript of interest in the tissue. The labeled probe is then visualized by immunohistochemical methods using an antibody conjugated to alkaline phosphatase that catalyzes a color reaction.
University of Rochester Medical Center, Rochester, NY, USA.
Timothy
T
Rutkowsky
University of Rochester Medical Center, Rochester, NY, USA.
Deepika
D
Sharma
University of Rochester Medical Center, Rochester, NY, USA.
Matthew J
MJ
Hilton
D000818:Animals; D001842:Bone and Bones / Q000196:embryology / Q000378:metabolism*; D005333:Fetus; D015870:Gene Expression; D007150:Immunohistochemistry; D017403:In Situ Hybridization / Q000379:methods*; D051379:Mice