Acute and sub-chronic toxicity studies of the extract of Thunberg Fritillary Bulb.
The aim of this study was to investigate the acute and sub-chronic toxicity of extract of Thunberg Fritillary Bulb. For the acute toxicity tests, graded doses of the extract were administered orally to mice. The animals were observed for toxic symptoms and mortality daily for 14days. In the sub-chronic toxicity study, rats were orally administered the extract at doses of 1 and 3mg/kg body weight (BW) for 26weeks. After 26weeks, the rats were sacrificed for hematological, biochemical and histological examination. In the acute toxicity tests, the estimated median lethal dosage (LD50) was 52.2mg/kg body weight in the mice. In the sub-chronic toxicity tests, a dose of 1mg/kg body weight presented no toxicity. Above the 1mg/kg dose, the main adverse signs observed in male rats were body or head tremor and spontaneous motor activity reduction. There were no other significant changes observed in hematology, blood biochemistry, organ weight and organ histology. The overall findings of this study indicate that the extract of Thunberg Fritillary Bulb is non-toxic up to 1mg/kg body weight, which can be considered a safe application dose.
Department of Pharmacology of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China; Department of Biomedicine Science, College of Medicine, University of Toledo, Toledo 43614, United States.
Zehui
Z
Li
Department of Pharmacology of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
Yuan
Y
Qiao
Drug Safety Evaluation Center, China Institute for Radiation Protection, Taiyuan 030006, China.
Jianguo
J
Li
Department of Oncology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China.
Chao
C
An
Department of Oncology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China.
Kaiwen
K
Hu
Department of Pharmacology of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. Electronic address: tangmk@bucm.edu.cn.
Minke
M
Tang
D000818:Animals; D005260:Female; D031410:Fritillaria; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D020258:Neurotoxicity Syndromes / Q000209:etiology; D010936:Plant Extracts / Q000633:toxicity*; D018517:Plant Roots; D017207:Rats, Sprague-Dawley; D023382:Toxicity Tests, Acute; D059550:Toxicity Tests, Subchronic; D014202:Tremor / Q000139:chemically induced
D010936:Plant Extracts
Acute toxicity; Sub-chronic toxicity; Thunberg Fritillary Bulb