Gut microbiome phenotypes driven by host genetics affect arsenic metabolism.
Large individual differences in susceptibility to arsenic-induced diseases are well-documented and frequently associated with different patterns of arsenic metabolism. In this context, the role of the gut microbiome in directly metabolizing arsenic and triggering systemic responses in diverse organs raises the possibility that gut microbiome phenotypes affect the spectrum of metabolized arsenic species. However, it remains unclear how host genetics and the gut microbiome interact to affect the biotransformation of arsenic. Using an integrated approach combining 16S rRNA gene sequencing and HPLC-ICP-MS arsenic speciation, we demonstrate that IL-10 gene knockout leads to a significant taxonomic change of the gut microbiome, which in turn substantially affects arsenic metabolism.
Department of Biological Engineering, ‡Division of Comparative Medicine, and §Department of Chemistry, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.
Kun
K
Lu
Ridwan
R
Mahbub
Peter Hans
PH
Cable
Hongyu
H
Ru
Nicola M A
NM
Parry
Wanda M
WM
Bodnar
John S
JS
Wishnok
Miroslav
M
Styblo
James A
JA
Swenberg
James G
JG
Fox
Steven R
SR
Tannenbaum
D000818:Animals; D001151:Arsenic / Q000493:pharmacokinetics*; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D004785:Environmental Pollutants / Q000493:pharmacokinetics*; D041981:Gastrointestinal Tract / Q000378:metabolism / Q000382:microbiology*; D016753:Interleukin-10 / Q000235:genetics*; D013058:Mass Spectrometry; D051379:Mice; D018345:Mice, Knockout; D064307:Microbiota; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004785:Environmental Pollutants; C556062:IL10 protein, mouse; D012336:RNA, Ribosomal, 16S; D016753:Interleukin-10; D001151:Arsenic