Targeting a rare amyloidotic disease through rationally designed polymer conjugates.
Saraiva et al. discovered in 2006 a RAGE-based peptide sequence capable of preventing transthyretin (TTR) aggregate-induced cytotoxicity, hallmark of initial stages of an inherited rare amyloidosis known as Familial Amyloidotic Polyneuropathy (FAP). To allow clinical progression of this peptidic sequence as FAP treatment, a family of polymer conjugates has been designed, synthesised and fully characterised. This approach fulfils the strategies defined in the Polymer Therapeutics area as an exhaustive physico-chemical characterisation fitting activity output towards a novel molecular target that is described here. RAGE peptide acts extracellularly, therefore, no intracellular drug delivery was necessary. PEG was selected as carrier and polymer-drug linker optimisation was then carried out by means of biodegradable (disulphide) and non-biodegradable (amide) covalent bonds. Conjugate size in solution, stability under in vitro and in vivo scenarios and TTR binding affinity through surface plasmon resonance (SPR) was also performed with all synthesised conjugates. In their in vitro evaluation by monitoring the activation of caspase-3 in Schwann cells, peptide derivatives demonstrated retention of peptide activity reducing TTR aggregates (TTRagg) cytotoxicity upon conjugation and a greater plasma stability than the parent free peptide. The results also confirmed that a more stable polymer-peptide linker (amide) is required to secure therapeutic efficiency.
Polymer Therapeutics Lab., Centro de Investigación Príncipe Felipe (CIPF), C/Eduardo Primo Yúfera 3, Valencia 46012, Spain.
Inmaculada
I
Conejos-Sánchez
Instituto de Biología Molecular e Celular (IBMC), Rua do Campo Alegre 823, Porto 4150-180, Portugal.
Isabel
I
Cardoso
Instituto de Biología Molecular e Celular (IBMC), Rua do Campo Alegre 823, Porto 4150-180, Portugal; Instituto de Ciências Biomédicas Abel Salazar (ICBAS), Largo Prof. Aber Salazar 2, Porto 4099-003, Portugal.
Maria J
MJ
Saraiva
Polymer Therapeutics Lab., Centro de Investigación Príncipe Felipe (CIPF), C/Eduardo Primo Yúfera 3, Valencia 46012, Spain. Electronic address: mjvicent@cipf.es.
María J
MJ
Vicent
D028227:Amyloid Neuropathies, Familial; D000818:Animals; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000737:chemistry; D011228:Prealbumin / Q000378:metabolism*; D051381:Rats; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000737:chemistry*; D012583:Schwann Cells / Q000187:drug effects / Q000378:metabolism
D010455:Peptides; D011228:Prealbumin; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic; D011092:Polyethylene Glycols; D053148:Caspase 3
Drug delivery; Drug design; Nanoconjugates; Polymer therapeutics; Polymer–drug conjugate; Rare diseases