Cre-dependent adeno-associated virus preparation and delivery for labeling neurons in the mouse brain.
Virus-mediated gene delivery is a powerful strategy for labeling and manipulating neurons in mammalian brains. A major drawback of this gene delivery method has been the lack of cell-type specificity. However, methods that combine Cre-knockin mice and Cre-activated adeno-associated virus (AAV) have now been developed to achieve high-level, stable, and cell-type-specific gene expression. Here, we describe a protocol for the design, production, and delivery of Cre-dependent AAVs. This method is simple and highly efficient, and allows chronic live imaging of defined classes of synapses in the mouse brain.
Z Josh
ZJ
Huang
Hiroki
H
Taniguchi
Miao
M
He
Sandra
S
Kuhlman
D000818:Animals; D001921:Brain / Q000378:metabolism*; D000229:Dependovirus / Q000145:classification / Q000378:metabolism*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D019426:Integrases / Q000378:metabolism*; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D012703:Serotyping
D049452:Green Fluorescent Proteins; C045073:Cre recombinase; D019426:Integrases