Ultrasound-assisted extraction of Mangiferin from Mango (Mangifera indica L.) leaves using response surface methodology.
Mangiferin is a xanthone widely distributed in higher plants showing antioxidative, antiviral, anticancer, antidiabetic, immunomodulatory, hepatoprotective and analgesic effects. In the present study, an ultrasonic-assisted extraction method was developed for the effective extraction of mangiferin from mango leaves. Some parameters such as ethanol concentration, liquid-to-solid ratio, extraction temperature, and extraction time were optimized by single-factor experiment and response surface methodology. The optimal extraction conditions were 44% ethanol, the liquid-to-solid ratio was 38:1, and extraction for 19.2 min at 60 °C under ultrasound irradiation of 200 W. Under optimal conditions, the yield of mangiferin was 58.46 ± 1.27 mg/g. The results obtained are helpful for the full utilization of mango leaves, and also indicated that ultrasonic-assisted extraction is a very useful method for the extraction of mangiferin from plant materials.
Department of Nutrition and Food Hygiene, School of Public Health, Guangdong Medical College, Dongguan 523808, China. zoutb@163.com.
Tang-Bin
TB
Zou
Department of Nutrition and Food Hygiene, School of Public Health, Guangdong Medical College, Dongguan 523808, China. 2284@gdmc.edu.cn.
En-Qin
EQ
Xia
Department of Nutrition and Food Hygiene, School of Public Health, Guangdong Medical College, Dongguan 523808, China. 1450@gdmc.edu.cn.
Tai-Ping
TP
He
Department of Sanitary Inspection, School of Public Health, Guangdong Medical College, Dongguan 523808, China. 2184@gdmc.edu.cn.
Ming-Yuan
MY
Huang
Department of Nutrition and Food Hygiene, School of Public Health, Guangdong Medical College, Dongguan 523808, China. 1314@gdmc.edu.cn.
Qing
Q
Jia
Department of Nutrition and Food Hygiene, School of Public Health, Guangdong Medical College, Dongguan 523808, China. chineseli@163.com.
Hua-Wen
HW
Li
D000431:Ethanol / Q000737:chemistry; D031022:Mangifera / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D013016:Sound; D013696:Temperature; D044004:Xanthones / Q000737:chemistry / Q000302:isolation & purification*
D044004:Xanthones; C013592:mangiferin; D000431:Ethanol