In vitro inhibition of the replication of classical swine fever virus by porcine Mx1 protein.
Classical swine fever virus (CSFV) is the causative pathogen of classical swine fever (CSF), a highly contagious disease of swine. Mx proteins are interferon-induced dynamin-like GTPases present in all vertebrates with a wide range of antiviral activities. Although Zhao et al. (2011) have reported that human MxA can inhibit CSFV replication, whether porcine Mx1 (poMx1) has anti-CSFV activity remains unknown. In this study, we generated a cell line designated PK-15/EGFP-poMx1 which expressed porcine Mx1 protein constitutively, and we observed that the proliferation of progeny virus in this cell line was significantly inhibited as measured by virus titration, indirect immune fluorescence assay, Q-PCR and Western blot. Furthermore, when PTD-poMx1 fusion protein expressed in Escherichia coli (Zhang et al., 2013) was used to treat CSFV-infected PK-15 cells, the results showed that PTD-poMx1 inhibited CSFV replication in a dose-dependent manner. Additionally, the proliferation of progeny virus was inhibited as measured by virus titration and Q-PCR. Overall, the results demonstrated that poMx1 effectively inhibited CSFV replication, suggesting that poMx1 may be a valuable therapeutic agent against CSFV infection.
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Dan-ni
DN
He
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Xiao-min
XM
Zhang
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Ke
K
Liu
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Ran
R
Pang
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Jin
J
Zhao
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: zhoubin@njau.edu.cn.
Bin
B
Zhou
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Pu-yan
PY
Chen
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D002460:Cell Line; D006692:Classical Swine Fever Virus / Q000187:drug effects / Q000502:physiology*; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D064547:Myxovirus Resistance Proteins / Q000378:metabolism* / Q000494:pharmacology; D011993:Recombinant Fusion Proteins / Q000378:metabolism / Q000494:pharmacology; D013552:Swine; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D064547:Myxovirus Resistance Proteins; D011993:Recombinant Fusion Proteins
Antiviral activity; Classical swine fever virus (CSFV); Porcine Mx1 (poMx1)