Photosystem 1 preparations from dark-and light-grown cells of the cyanobacterium, Chlorogloea fritschii.
Photosystem 1 (PS1) enriched preparations have been extracted from the cyanobacterium Chlorogloea fritschii grown either in darkness or in the light. Absorption spectra show that the main chlorophyll peak has shifted from 678 nm in PS1 from light grown cells to 675 nm in PS1 from dark grown cells. Fluorescence spectra show a similar blue shift in wavelength maximum from 690 nm to 678 nm and the fluorescence intensity is higher in PS1 from dark grown cells. Allophycocyanin is present in PS1 from light grown cells, but absent from preparations from C. fritschii grown in the dark. P700: chlorophyll a ratios of the preparations from light and dark grown cells are 1:35 and 1:80 respectively, all P700 being photoactive. The results are interpreted to suggest that allophycocyanin is not attached to PS1 in dark grown C. fritschii, neither is all chlorophyll arranged in such a way as to ensure efficient energy transfer to P700.
Biology Division, Preston Polytechnic, Corporation Street, Preston, Lancashire, U.K..
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Hilary Evans