Differential expression and ligand binding indicate alternative functions for zebrafish polymeric immunoglobulin receptor (pIgR) and a family of pIgR-like (PIGRL) proteins.
The polymeric immunoglobulin (Ig) receptor (pIgR) is an integral transmembrane glycoprotein that plays an important role in the mammalian immune response by transporting soluble polymeric Igs across mucosal epithelial cells. Single pIgR genes, which are expressed in lymphoid organs including mucosal tissues, have been identified in several teleost species. A single pigr gene has been identified on zebrafish chromosome 2 along with a large multigene family consisting of 29 pigr-like (PIGRL) genes. Full-length transcripts from ten different PIGRL genes that encode secreted and putative inhibitory membrane-bound receptors have been characterized. Although PIGRL and pigr transcripts are detected in immune tissues, only PIGRL transcripts can be detected in lymphoid and myeloid cells. In contrast to pIgR which binds Igs, certain PIGRL proteins bind phospholipids. PIGRL transcript levels are increased after infection with Streptococcus iniae, suggesting a role for PIGRL genes during bacterial challenge. Transcript levels of PIGRL genes are decreased after infection with Snakehead rhabdovirus, suggesting that viral infection may suppress PIGRL function.
Department of Molecular Biomedical Sciences and Center for Comparative Medicine and Translational Research, North Carolina State University, 1060 William Moore Drive, Raleigh, NC, 27607, USA.
Amanda N
AN
Kortum
Ivan
I
Rodriguez-Nunez
Jibing
J
Yang
Juyoung
J
Shim
Donna
D
Runft
Marci L
ML
O'Driscoll
Robert N
RN
Haire
John P
JP
Cannon
Poem M
PM
Turner
Ronda T
RT
Litman
Carol H
CH
Kim
Melody N
MN
Neely
Gary W
GW
Litman
Jeffrey A
JA
Yoder
D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D017124:Conserved Sequence; D019143:Evolution, Molecular; D005399:Fishes / Q000235:genetics / Q000276:immunology; D015870:Gene Expression; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics; D008024:Ligands; D008322:Mammals / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D005810:Multigene Family; D010743:Phospholipids / Q000378:metabolism; D010802:Phylogeny; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D019056:Receptors, Polymeric Immunoglobulin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D018353:Rhabdoviridae Infections / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013290:Streptococcal Infections / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D015027:Zebrafish / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism
D008024:Ligands; D010743:Phospholipids; D019056:Receptors, Polymeric Immunoglobulin; D029961:Zebrafish Proteins