Detecting histone modifications in plants.
Histone modifications play an essential role in chromatin-associated processes including gene regulation and epigenetic inheritance. It is therefore very important to quantitatively analyze histone modifications at both the single gene and whole genome level. Here, we describe a robust chromatin immunoprecipitation (ChIP) method for Arabidopsis, which could be adapted for other plant species. This method is compatible with multiple downstream applications including qPCR, tilling arrays, and high-throughput sequencing.
Cell & Developmental Biology, John Innes Centre, Imperial College London, London, SW7 2AZ, UK.
Jie
J
Song
Bas
B
Rutjens
Caroline
C
Dean
D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D002843:Chromatin / Q000235:genetics; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D053938:DNA Fragmentation; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D005557:Formaldehyde / Q000494:pharmacology; D006657:Histones / Q000378:metabolism*; D011499:Protein Processing, Post-Translational
D002843:Chromatin; D018744:DNA, Plant; D006657:Histones; D005557:Formaldehyde