Astaxanthin reduces matrix metalloproteinase expression in human chondrocytes.
Astaxanthin is a red carotenoid pigment which exerts multiple biological activities. However, little is known about the effects of astaxanthin on matrix metalloproteinases (MMPs) in OA. The present study investigated the effects of astaxanthin on MMPs in human chondrocytes. Human chondrocytes were pretreated with astaxanthin at 1, 10 or 50μM, then, cells were stimulated with IL-1β (10ng/ml) for 24h. MMP-1, MMP-3 and MMP-13 were observed. We found that astaxanthin reduced the expression of MMP-1, MMP-3 and MMP-13 as well as the phosphorylation of two mitogen-activated protein kinases (MAPK) (p38 and ERK1/2) in IL-1β-stimulated chondrocytes. Astaxanthin also blocked the IκB-α degradation. These results suggest that astaxanthin may be beneficial in the treatment of OA.
Department of Orthopedic Surgery, the Second Affiliated Hospital, School of Medicine, Zhejiang University.
Wei-Ping
WP
Chen
Department of Orthopedic Surgery, the Second Affiliated Hospital, School of Medicine, Zhejiang University.
Yan
Y
Xiong
Department of Orthopedic Surgery, the Second Affiliated Hospital, School of Medicine, Zhejiang University.
Yong-Xiang
YX
Shi
Department of Orthopedic Surgery, the Second Affiliated Hospital, School of Medicine, Zhejiang University.
Peng-Fei
PF
Hu
Department of Orthopedic Surgery, the Second Affiliated Hospital, School of Medicine, Zhejiang University.
Jia-Peng
JP
Bao
Department of Orthopedic Surgery, the Second Affiliated Hospital, School of Medicine, Zhejiang University. Electronic address: ldwu@yahoo.com.
Li-Dong
LD
Wu
D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D020672:I-kappa B Proteins / Q000378:metabolism; D053583:Interleukin-1beta; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism; D053509:Matrix Metalloproteinase 13 / Q000235:genetics / Q000378:metabolism; D019278:Matrix Metalloproteinase 3 / Q000235:genetics / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D000072000:NF-KappaB Inhibitor alpha; D024341:Xanthophylls / Q000494:pharmacology
D000893:Anti-Inflammatory Agents; D020672:I-kappa B Proteins; D053583:Interleukin-1beta; C000606204:NFKBIA protein, human; D024341:Xanthophylls; D000072000:NF-KappaB Inhibitor alpha; C005948:astaxanthine; D020928:Mitogen-Activated Protein Kinases; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13; C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1
Astaxanthin; Matrix metalloproteinase; Osteoarthritis