Revealing individual signatures of human T cell CDR3 sequence repertoires with Kidera Factors.
The recent development of High Throughput Sequencing technologies has enabled an individual's TCR repertoire to be efficiently analysed at the nucleotide level. However, with unique clonotypes ranging in the tens of millions per individual, this approach gives a surfeit of information that is difficult to analyse and interpret in a biological context and gives little information about TCR structural diversity. Using publicly available TCR CDR3 sequence data, we analysed TCR repertoires by converting the encoded CDR3 amino acid sequences into Kidera Factors, a set of orthogonal physico-chemical properties that reflect protein structure. This approach enabled the TCR repertoire from different individuals to be distinguished and demonstrated the close similarity of the repertoire in different samples from the same individual.
Department of Statistical Science, University College London, London, United Kingdom ; CoMPLEX, University College London, London, United Kingdom.
Michael
M
Epstein
CoMPLEX, University College London, London, United Kingdom ; Institute of Child Health, University College London, London, United Kingdom.
Martino
M
Barenco
Institute of Child Health, University College London, London, United Kingdom.
Nigel
N
Klein
CoMPLEX, University College London, London, United Kingdom ; Institute of Child Health, University College London, London, United Kingdom.
Michael
M
Hubank
CoMPLEX, University College London, London, United Kingdom ; Institute of Child Health, University College London, London, United Kingdom.
Robin E
RE
Callard
D000595:Amino Acid Sequence; D005260:Female; D006801:Humans; D007156:Immunologic Memory; D008297:Male; D008969:Molecular Sequence Data; D025341:Principal Component Analysis; D011487:Protein Conformation; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000737:chemistry* / Q000145:classification / Q000276:immunology; D013601:T-Lymphocytes / Q000737:chemistry* / Q000276:immunology
D016693:Receptors, Antigen, T-Cell, alpha-beta