Arp2/3 complex inhibition prevents meiotic maturation in porcine oocytes.
The Arp2/3 complex regulates actin nucleation, which is critical for a wide range of cellular processes, such as cell polarity, cell locomotion, and endocytosis. In the present study, we investigated the possible roles of the Arp2/3 complex in porcine oocytes during meiotic maturation. Immunofluorescent staining showed the Arp2/3 complex to localize mainly to the cortex of porcine oocytes, colocalizing with actin. Treatment with an Arp2/3 complex specific inhibitor, CK666, resulted in a decrease in Arp2/3 complex localization at the oocyte cortex. The maturation rate of porcine oocytes decreased significantly after CK666 treatment, concomitant with the failure of cumulus cell expansion and oocyte polar body extrusion. The fluorescence intensity of F-actin decreased in the cytoplasm, and CK666 also disrupted actin cap formation. In summary, our results illustrate that the Arp2/3 complex is required for the meiotic maturation of porcine oocytes and that actin nucleation is critical for meiotic maturation.
College of Animal Sciences and Technology, Nanjing Agricultural University, Nanjing, China.
Fei
F
Wang
Department of Animal Sciences, Chungbuk National University, Cheongju, Korea.
Ga-Young
GY
An
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
Yu
Y
Zhang
College of Animal Sciences and Technology, Nanjing Agricultural University, Nanjing, China.
Hong-Lin
HL
Liu
Department of Animal Sciences, Chungbuk National University, Cheongju, Korea.
Xiang-Shun
XS
Cui
Department of Animal Sciences, Chungbuk National University, Cheongju, Korea.
Nam-Hyung
NH
Kim
College of Animal Sciences and Technology, Nanjing Agricultural University, Nanjing, China.
Shao-Chen
SC
Sun
D051376:Actin-Related Protein 2-3 Complex / Q000378:metabolism*; D000818:Animals; D054885:Cumulus Cells / Q000166:cytology / Q000378:metabolism; D005260:Female; D008540:Meiosis / Q000502:physiology*; D059705:Polar Bodies / Q000166:cytology / Q000378:metabolism*; D013552:Swine
D051376:Actin-Related Protein 2-3 Complex