Characterization of polymorphic microsatellite loci in the Antarctic krill Euphausia superba.
BACKGROUND
The Antarctic krill, Euphausia superba is a pelagic crustacean, abundant in high-density swarms (10,000 - 30,000 ind/m2) with a circumpolar distribution and a key role in the food web of the Southern Ocean. Only three EST derived microsatellite markers have been used in previous genetic studies, hence we developed additional highly polymorphic microsatellite markers to allow robust studies of the genetic variability and population differentiation within this species.


RESULTS
The microsatellite markers described here were obtained through an enriched genomic library, followed by 454 pyrosequencing. A total of 10 microsatellite markers were tested in 32 individuals from the Antarctic Peninsula. One of the tested loci was fixed for one allele while the other was variable. Of the remaining nine markers, seven showed no departure from Hardy-Weinberg equilibrium. The mean number of alleles was 14.9.


CONCLUSIONS
These markers open perspectives for population genetic studies of this species to unravel genetic structure, dispersal and population biology, vital information for future conservation.
Rui
R
Candeias
Centre of Marine Sciences, CCMAR-CIMAR, University of the Algarve, Gambelas Campus, 8005-139 Faro, Portugal. steixeira@ualg.pt.
Sara
S
Teixeira
Carlos M
CM
Duarte
Gareth A
GA
Pearson
D000483:Alleles; D000818:Animals; D000864:Antarctic Regions; D001483:Base Sequence; D033201:Euphausiacea / Q000235:genetics*; D020224:Expressed Sequence Tags; D005819:Genetic Markers; D014644:Genetic Variation; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic
D005819:Genetic Markers