Transcriptional regulation of abscisic acid signal core components during cucumber seed germination and under Cu²⁺, Zn²⁺, NaCl and simulated acid rain stresses.
Abscisic acid (ABA) is an important phytohormone that regulates lots of physiological and biochemical processes in plant life cycle, especially in seed germination and stress responses. For exploring the transcriptional regulation of ABA signal transduction during cucumber (Cucumis sativus L.) seed germination and under Cu(2+), Zn(2+), NaCl and simulated acid rain stresses, nine CsPYLs, three group A CsPP2Cs and two subclass III CsSnRK2s were identified from cucumber genome, which respectively showed high sequence similarities and highly conserved domains with homologous genes in Arabidopsis. Based on Real-time PCR analysis, most of the tested genes' expression decreased during cucumber seed germination, which was in accordance with the ABA level variation. In addition, according to the absolute expression, CsPYL1, CsPYL3, CsPP2C5, CsABI1, CsSnRK2.3 and CsSnRK2.4 were highly expressed, indicating that they may play more important roles in ABA signaling during cucumber seed germination. Moreover, most of these highly expressed genes, except CsPYL3, were up-regulated by ABA treatment. Meanwhile, most of the tested genes' expression dramatically changed at the initial water uptake phase, indicating that this period may be critical in the regulation of ABA on seed germination. Under Cu(2+), Zn(2+), NaCl and simulated acid rain stresses, cucumber seed germination percentage decreased and ABA content increased. Meanwhile, the expression of ABA signal transduction core components genes showed specific response to a particular stress and was not always consist with ABA variation. Generally, the expression of CsPYL1, CsPYL3, CsABI1, CsSnRK2.3 and CsSnRK2.4 was sensitive to 120 mM NaCl and 0.5 mM Cu(2+) treatments.
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China.
Yanping
Y
Wang
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China.
Ya
Y
Wang
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China.
Wenbin
W
Kai
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China.
Bo
B
Zhao
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China.
Pei
P
Chen
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China.
Liang
L
Sun
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China.
Kai
K
Ji
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China.
Qian
Q
Li
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China.
Shengjie
S
Dai
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China.
Yufei
Y
Sun
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China.
Yidong
Y
Wang
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China.
Yuelin
Y
Pei
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China. Electronic address: pleng@cau.edu.cn.
Ping
P
Leng
D000040:Abscisic Acid / Q000378:metabolism*; D015258:Acid Rain; D003300:Copper / Q000737:chemistry*; D018553:Cucumis sativus / Q000187:drug effects / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D018525:Germination; D010940:Plant Proteins / Q000378:metabolism; D012639:Seeds / Q000502:physiology; D015398:Signal Transduction; D012965:Sodium Chloride / Q000737:chemistry*; D013312:Stress, Physiological; D015032:Zinc / Q000737:chemistry*
D015258:Acid Rain; D010940:Plant Proteins; D012965:Sodium Chloride; D000040:Abscisic Acid; D003300:Copper; D015032:Zinc
ABA signal transduction; Cu(2+); Cucumber seed germination; Expression analysis; NaCl; Simulated acid rain; Zn(2+)