Validation of spectrophotometric method for Se(IV) determination: analytical applications.
As selenium is an important part of the antioxidant enzymes and also because there are several studies suggesting a possible link between cancer and selenium deficiency, this paper presents a spectrophotometric method for the assay of Se(IV), using N,N-diethyl-p-phenylenediamine monohydrochloride as reagent. The proposed method is based on the reaction between the selenium and potassium iodide in low acidic medium, when iodine is released. This last product will further oxidise the new reagent. The final obtained product is strongly coloured in red and has an absorption maximum at 552 nm and molar extinction coefficient (ε) of 6.1 × 10(4) L mol(-1) cm(-1). The optimum working conditions were established, and the developed method was validated, being characterised by a good linearity (in the range of 0.5-3.0 μg/mL), a limit of detection (0.0573 μg/mL) and a limit of quantification (0.1737 μg/mL). At the same time, the repeatability, the precision of the method and the accuracy were established. The proposed and validated method was applied with good results for the determination of Se(IV) in spring and bottled water from Iasi and also in pharmaceutical and cosmetic products.
Faculty of Pharmacy, "Grigore T. Popa" University of Medicine and Pharmacy, Iasi, Romania.
Gladiola
G
Tantaru
Madalina
M
Vieriu
Maria-Cristina
MC
Popescu
D004784:Environmental Monitoring; D006863:Hydrogen-Ion Concentration; D007454:Iodides / Q000032:analysis; D007455:Iodine / Q000032:analysis; D057230:Limit of Detection; D010655:Phenylenediamines; D012643:Selenium / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods; D014131:Trace Elements / Q000032:analysis*
D007454:Iodides; D010655:Phenylenediamines; D014131:Trace Elements; D007455:Iodine; D012643:Selenium; C029728:4-phenylenediamine