[Effects of soluble egg antigen and adult worm antigen of Schistosoma japonicum on differentiation of effector B cells of mice].
OBJECTIVE
To observe the differentiation ability of effector B cells induced by soluble egg antigen (SEA) and soluble adult worm antigen (SWAP) of Schistosoma japonicum.


METHODS
The mouse spleen mononuclear cells and CDl9+ B cells sorted by beadg were stimulated by SEA, SWAP and lipopolysaccharide (LPS), respectively. The ratios of CD19+ IL-6+ cells and CD19+ IFN-gamma+ cells were analyzed by flow cytometry after 72 hours. At the same time, the cytokines IL-6 and IFN-gamma levels in the cultured supernatants were detected by ELISA. The mouse was immunized with the mixture of SEA or SWAP or LPS and the incomplete Freund' s adjuvant for three times, respectively. The mouse spleen mononuclear cells were isolated at the seventh day after the last immunization. The ratios of CD19+ IL-6+ cells and CD19 IFN-gama+ cells were analyzed, and the cytokines IL-6 and IFN-gamma+ levels in the culture supernatants were detected.


RESULTS
The ratio of CD19 IL-6+ cells in spleen mononuclear cells and splenic B cells was significantly increased in the groups stimulated by SEA and LPS (P < 0.05), and the cytokines IL-6 level in the CD193 cells culture supernatants were significantly increased (P < 0.01). Furthermore, the ratio of CD19+ IL-6+ cells and the cytokines IL-6 level were significantly increased in the SEA immunized group (P < 0.01). SWAP could induce a significantly higher ratio of the CD19+ IFN-gamma+ cells in spleen cells, instead of in splenic CD19+ B cells (P < 0.05). The CD19+ IFN-gamma+ cells and the cytokine IFN-gamma level in the culture supernatants in the SWAP immunized group were significantly higher than those in the SEA and PBS immunized groups (P C 6.01).


CONCLUSIONS
SEA preferentially induces the increase of CDl9+ IL-6+ cells in mouse spleen cells; while SWAP preferentially induces the CD19 + IFN-gamma+ cells' production of mouse spleen cells, depending on the effects of other immune cells.
Department of Pathogen Biology and Immunology, Yangzhou University Medical College, Yangzhou 225001, China. fangtiantf@163.com
Fang
F
Tian
Department of Pathogen Biology and Immunology, Yangzhou University Medical College, Yangzhou 225001, China.
Xue-li
XL
Hu
Department of Pathogen Biology and Immunology, Yangzhou University Medical College, Yangzhou 225001, China.
Hao
H
Liu
Department of Pathogen Biology and Immunology, Yangzhou University Medical College, Yangzhou 225001, China.
Hui
H
Wei
Department of Pathogen Biology and Immunology, Yangzhou University Medical College, Yangzhou 225001, China.
Qiu-fang
QF
Duan
Department of Pathogen Biology and Immunology, Yangzhou University Medical College, Yangzhou 225001, China.
Li
L
Qian
Department of Pathogen Biology and Immunology, Yangzhou University Medical College, Yangzhou 225001, China.
Hua
H
Yan
Department of Pathogen Biology and Immunology, Yangzhou University Medical College, Yangzhou 225001, China.
Wei-ping
WP
Yang
D000375:Aging; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D001402:B-Lymphocytes / Q000166:cytology*; D002454:Cell Differentiation; D005260:Female; D007371:Interferon-gamma / Q000032:analysis; D015850:Interleukin-6 / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D010063:Ovum / Q000276:immunology; D012549:Schistosoma japonicum / Q000276:immunology*
D000947:Antigens, Helminth; D015850:Interleukin-6; D007371:Interferon-gamma