Probing the selective antitumor activity of 22-oxo-26-selenocyanocholestane derivatives.
Diverse steroidal compounds have shown antiproliferative activity on certain tumor cell lines; however, their complete role on cancer cells has not been extensively established since the research is quite recent. Hence, deeper study in this field is required. Due to the importance of selenium in animal and human health; herein, we report the synthesis, characterization, and biological evaluation of two novel 22-oxo-26-selenocyanocholestanic steroids on cervicouterine cancer cells and non-tumor cells. The title compounds were straightforward prepared from diosgenin and hecogenin in excellent overall yields. We determined their effect on cell proliferation on HeLa, CaSki, and ViBo cell cultures. Their cytotoxic effect on tumor cells, as well as on peripheral blood lymphocytes was also evaluated. The increase in the expression of active caspase-3 along with the fragmentation of DNA confirm that the new 22-oxo-26-selenocyanocholestane frameworks potentiate apoptosis in tumor cells. The antiproliferative activity on tumor cells affects to some extent the proliferative potential of peripheral blood lymphocytes, so an immunosuppressive effect has also been established. The novel 22-oxo-26-selenocyanocholestane compounds show selective antitumor activity and therefore are promising lead candidates for further in vivo evaluation.
Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, Ciudad Universitaria, 72570 Puebla, PUE, Mexico. Electronic address: marieta.fernandez@correo.buap.mx.
María A
MA
Fernández-Herrera
Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, Ciudad Universitaria, 72570 Puebla, PUE, Mexico.
Jesús
J
Sandoval-Ramírez
Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, 09230 México, D. F., Mexico.
Luis
L
Sánchez-Sánchez
Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, 09230 México, D. F., Mexico.
Hugo
H
López-Muñoz
Departamento de Biología Celular, Facultad de Ciencias, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510 México, D. F., Mexico.
María L
ML
Escobar-Sánchez
D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002778:Cholestanones / Q000737:chemistry / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D015335:Molecular Probes; D016566:Organoselenium Compounds / Q000737:chemistry / Q000494:pharmacology*
D000970:Antineoplastic Agents; D002778:Cholestanones; D015335:Molecular Probes; D016566:Organoselenium Compounds
22-Oxo-26-selenocyanocholestanes; Antiproliferative activity; Apoptosis; Cervicouterine cancer cells; Cytotoxicity