Ability of barrier coat S-PRG coating to arrest artificial enamel lesions in primary teeth.
OBJECTIVE
To evaluate the effects of a surface pre-reacted glass-ionomer (S-PRG) filled coating material to arrest artificial enamel lesions in primary teeth.


METHODS
Buccal and lingual enamel was demineralized in 0.1 M lactic acid buffer solution (pH 4.75) for 5 days and then divided in the PRG-applied and non-PRG areas. Proximal surfaces were used as a control area without demineralization and coating application. Teeth were divided into three groups (n = 4) according to the 1-week immersion in different solutions: Group 1 (distilled water), Group 2 (demineralizing solution) and Group 3 (artificial saliva). Hardness and Young's modulus by nano-indentation test, and elemental contents and ultrastructure by SEM/EDX analysis were obtained. Data were statistically analyzed using ANOVA and Fisher's PLSD at alpha = 0.05.


RESULTS
Only for the non-PRG area in Group 1, the hardness and Young's modulus of the demineralized surface enamel were significantly lower than those of the enamel 30-60 microm beneath the surface. Demineralized enamel of non-PRG and PRG-applied areas showed similar SEM views. Only for the non-PRG area in Group 2 and control area in Group 3, the Ca/P of the surface enamel was significantly higher than that of the enamel 5-10 microm beneath the surface. There was no significant difference of the Ca/P among the measuring points from the surface to 10 microm depth of enamel for the PRG applied area in Group 2.
Department of Pediatric Dentistry, Unit of Translational Medicine, Course of Medical and Dental Science, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1, Sakamoto, Nagasaki 852-8588, Japan. hosoya@nagasaki-u.ac.jp
Yumiko
Y
Hosoya
Department of Operative Dentistry, Nihon University School of Dentistry, Tokyo, Japan.
Susumu
S
Ando
Otani Dental Clinic, Nayoro, Japan.
Hideji
H
Otani
Yukinari Pediatric and Orthodontic Dental Clinic, Nagasaki, Japan.
Tetsuhiro
T
Yukinari
Department of Operative Dentistry, Nihon University School of Dentistry, Tokyo, Japan.
Masashi
M
Miyazaki
Bioscience Research Center, College of Dentistry, University of Tennessee, Memphis, USA.
Franklin
F
Garcia-Godoy
D002118:Calcium / Q000032:analysis; D002327:Cariostatic Agents / Q000627:therapeutic use*; D020099:Coated Materials, Biocompatible / Q000627:therapeutic use*; D003731:Dental Caries / Q000517:prevention & control*; D003743:Dental Enamel / Q000187:drug effects*; D055119:Elastic Modulus; D004577:Electron Probe Microanalysis; D005899:Glass Ionomer Cements / Q000627:therapeutic use*; D006244:Hardness; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010758:Phosphorus / Q000032:analysis; D011092:Polyethylene Glycols / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry; D019279:Resin Cements / Q000627:therapeutic use*; D012464:Saliva, Artificial / Q000737:chemistry; D017001:Tooth Demineralization / Q000517:prevention & control; D014094:Tooth, Deciduous / Q000187:drug effects*; D014867:Water / Q000737:chemistry
D002327:Cariostatic Agents; D020099:Coated Materials, Biocompatible; D005899:Glass Ionomer Cements; D011109:Polymethacrylic Acids; D019279:Resin Cements; D012464:Saliva, Artificial; D014867:Water; C020946:triethylene glycol dimethacrylate; D010758:Phosphorus; D011092:Polyethylene Glycols; D002118:Calcium