[Expression change of SH2B1, SOCS3, PTP1B and NPY in mice hypothalamus and its relation with obesity].
OBJECTIVE
To investigate the expression pattern of adapter protein with a Src-homology 2 domain (SH2B1), the suppressor of cytokine signaling-3 (SOCS3), protein-tyrosine phosphatase 1B (PTP1B) and neturopetide Y (NPY) in obese and normal mice hypothalamus and its relation with serum leptin and insulin levels.


METHODS
The obesity animal model was prepared with healthy C57/bl6 mice. Lee's index and Homeostasis model assessment-insulin resistance (HOMA-IR) were calculated. The mRNA levels of SH2B1, SOCS3, PTP1B and NPY were measured by fluorescent quantitation RT-PCR. The SH2B1 and NPY protein expressions were detected by Western blot.


RESULTS
Compared with the normal mice of the same age, SH2B1 mRNA expression in the obese mice hypothalamus decreased. SOCS3 and PTP1B mRNA expression increased. Western blot showed that SH2B1 protein expression decreased, while NPY protein expression increased in the obese mice. Linear correlation analysis showed that the serum leptin and fasting insulin levels were negatively correlated with SH2B1mRNA expression and positively correlated with SOCS3 and PTP1B mRNA expression.


CONCLUSIONS
SH2B1, SOCS3, PTP1B and NPY are key factors for obesity development.
Institute of Medical Sciences, Xiangya Hospital, Central South University, Changsha 410008, China.
Tao
T
Su
Jing
J
Wu
Weifang
W
Liu
Chaojun
C
Duan
Sai
S
Zhang
Can'e
C
Tang
Fanyan
F
Luo
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000818:Animals; D007031:Hypothalamus / Q000378:metabolism*; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D020738:Leptin / Q000097:blood; D051379:Mice; D008810:Mice, Inbred C57BL; D009478:Neuropeptide Y / Q000378:metabolism*; D009765:Obesity / Q000378:metabolism*; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1 / Q000378:metabolism*; D012333:RNA, Messenger; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; D007328:Insulin; D020738:Leptin; D009478:Neuropeptide Y; D012333:RNA, Messenger; C115327:Sh2bpsm1 protein, mouse; C491411:Socs3 protein, mouse; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; C516609:Ptpn1 protein, mouse