[Relations between enzymes which build up flavonols, enzymes which convert flavonols, and the accumulation of phenylpropanoid compounds during the development of anthers].
The difference in activity of enzymes which are involved in the biosynthesis of flavonols (phenylalanineammonium-lyase, chalcone-flavanone isomerase) and of an enzyme which converts flavonols is studied during the development of anthers in Tulipa cv. Apeldoorn. The results are considered in relation to the accumulation of simple phenylpropanes, of an intermediate a chalcone and of different flavonoid compounds.In the stages of development with high activities of flavonol synthesizing enzymes there are also high activities in the flavonol converting enzymes. In these stages a large amount of derivates of p-coumaric acid and ferulic acid is accumulated as well as the intermediate product 2',3,4,4',6'-pentahydroxychalcone; accordingly only traces of flavonols can be found.An intensive accumulation of different flavonols does not start before the late phase of development of the anthers in which the stationary concentration of chalcones decreases. It is the stage when the activity of the flavonol converting enzyme decreases rapidly.The relations between the stationary concentration of flavonoid compounds and the interlacing of synthesis and turnover are discussed.
Botanisches Institut der Universität, Schloßgarten 3, D-4400, Münster, Bundesrepublik Deutschland.
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R
Wiermann