[The protective effect of asiatic acid against oxygen-glucose deprivation/reoxygenation injury of PC12 cells].
To study the protective effect and preliminary mechanisms of asiatic acid against oxygen-glucose deprivation/reoxygenation (OGD/R) injury of PC12 cells, Na2S2O4 combined with low glucose induced damage of PC12 cells was served as OGD/R injury model in vitro. MTT method was used to evaluate cell survival. Ultraviolet spectrophotometry was performed to determine lactate dehydrogenase (LDH) leakage, lactic acid (LD) content, intracellular superoxide dismutase (SOD), malonyldialdehyde (MDA), and cellular Caspase-3 activity. Flow cytometry was applied to assay cell apoptosis. Na2S2O4 combined with low glucose induced significant cell survival rate decreasing compared with normal cells. Cell survival rate increasing, LDH leakage alleviating, LD producing inhibiting, SOD activity promotion, MDA content reducing, cell apoptotic rate decreasing and Caspase-3 activity inhibiting were observed when cells were preincubated with different concentration of asiatic acid (10, 1 and 0.1 micromol x L(-1)). Evident protective effect of asiatic acid against OGD/R injured PC12 cells was verified in our experiment, and the possible mechanisms were related to eliminating free radicals and inhibiting cell apoptosis.
Pharmacy Department, Affiliated Hospital of Nantong University, Nantong 226001, China.
Ju-ping
JP
Yuan
Blood Bank, Affiliated Hospital of Nantong University, Nantong 226001, China.
Jian-ming
JM
Lu
Cardiac Functiom Room, Affiliated Hospital of Nantong University, Nantong 226001, China.
Yuan
Y
Lu
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D028041:Centella / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D010100:Oxygen / Q000378:metabolism*; D016716:PC12 Cells; D053978:Pentacyclic Triterpenes / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D015427:Reperfusion Injury / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
D018696:Neuroprotective Agents; D053978:Pentacyclic Triterpenes; D019344:Lactic Acid; D008315:Malondialdehyde; C017032:asiatic acid; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase; C505410:Casp3 protein, rat; D053148:Caspase 3; D005947:Glucose; D010100:Oxygen