[Effects of micron Chinese herbal Liu-He-Dan ointment on inflammation and oxidative stress in rats with acute pancreatitis].
OBJECTIVE
To evaluate the effects of micron Chinese herbal Liu-He-Dan ointment (MLHD) on inflammation and oxidative stress in rats with acute pancreatitis (AP).


METHODS
Eighteen SD rats were randomized into three groups: normal group (n = 6), AP group (n = 6) and MLHD group (n = 6). The rat model of AP was induced by intra-peritoneal injection of 150 g/L L-arginine with dosage of 2.0 mg/g twice within 2 hours. As control, the normal rats were injected the same amount of saline. The MLHD was applied to the abdomen area of the AP rats in MLHD group for 72 h. The serum and pancreas of the rats were obtained. The level of amylase, TNF-alpha, IL-6, IL-10 in serum and SOD, MDA in the pancreas were detected and quantified.


RESULTS
Compared with AP group, the serum levels of amylase, TNF-alpha, IL-6, IL-10 were significantly decreased in MLHD group (P < 0.05). And the SOD in pancreas increased significantly (P < 0.05), but a decreasing trend of MDA level in MLHD group (P > 0.05) was observed. The serum level of the pathological score of pancreas in treatment group were similar to that in the model group (P > 0.05).


CONCLUSIONS
Micron Liu-He-Dan ointment may have therapeutic and protective effects on AP by antioxidation and alleviating inflammatory response.
Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Xiao-Hang
XH
Peng
Department of Acupuncture and Moxibustion, Chengdu University of Traditional Chinese Medicine, Chengdu 610072, China.
Weiz
W
Huang
Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Lin
L
Zhu
Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Qing
Q
Xia
Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Wen-Fu
WF
Tang
D000279:Administration, Cutaneous; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D001120:Arginine; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D007249:Inflammation / Q000188:drug therapy; D008297:Male; D009824:Ointments; D018384:Oxidative Stress / Q000502:physiology*; D010195:Pancreatitis / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology; D008517:Phytotherapy; D051381:Rats; D017207:Rats, Sprague-Dawley
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; C581590:Liu-He-Dan; D009824:Ointments; D001120:Arginine