Gold nanoparticle-based exonuclease III signal amplification for highly sensitive colorimetric detection of folate receptor.
By combining terminal protection of small molecule (folate)-capped DNA probes, exonuclease III signal amplification and gold nanoparticles, we developed a simple and label-free colorimetric assay for highly sensitive detection of folate receptor (FR). A detection limit of 50 fM FR was obtained using UV-vis spectrometry and 10 pM FR could be visualized by the naked eye.
Department of Chemistry, National University of Singapore, Singapore 117543. chmgaoz@nus.edu.sg.
Xinjian
X
Yang
Zhiqiang
Z
Gao
D015342:DNA Probes / Q000737:chemistry; D005090:Exodeoxyribonucleases / Q000737:chemistry / Q000378:metabolism*; D058973:Folate Receptors, GPI-Anchored / Q000032:analysis*; D005492:Folic Acid / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D021141:Nucleic Acid Amplification Techniques; D013056:Spectrophotometry, Ultraviolet
D015342:DNA Probes; D058973:Folate Receptors, GPI-Anchored; D006046:Gold; D005492:Folic Acid; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III