[Effect of growth factors and some microelements on biosurfactant synthesis of Acinetobacter calcoaceticus IMV B-7241].
The effect of yeast autolysate and microelements on synthesis of surface-active substances (SAS, biosurfactants) was investigated under cultivation of Acinetobacter calcoaceticus IMV B-7241 on various carbon substrates (n-hexadecane, ethanol, glycerol). The authors have shown a possibility to substitute the yeast autolysate and microelement mixture in the composition of ethanol- and n-hexadecane-containing media by copper sulfate (0.16 micromol/l) and iron sulfate (3.6 micromol/l), and in the medium with glycerol by 0.21 mmol/l of KCl, 38 micromol/l of zinc sulfate and 0.16 micromol/l of copper sulfate. Under such conditions of cultivation of the strain IMV B-7241 the SAS concentration exceeded that on the initial media, which contained the yeast autolysate and microelements, 1.2-1.6 times. The authors have also established the activating effect of low (0.01 mM) concentrations of Fe2+ on activity of the enzymes of biosynthesis of surface-active amino- (NADP-dependent glutamate dehydrogenase) and glycolipids (phosphoenolpyruvate(PhEP)-synthetase, PhEP-carboxykinase), as well as of anaplerotic reaction(PhEP-carboxylase). A necessity to introduce zinc cations into glycerol-containing medium is determined by their stimulating effect on activity of 4-dinitroso-N,N-dimethylaniline-dependent alcohol dehydrogenase--one of the enzymes of this substrate catabolism in A. calcoaceticus IMV B-7241.
T P
TP
Pirog
T A
TA
Shevchuk
O Iu
OIu
Mashchenko
S A
SA
Parfeniuk
G A
GA
Iutinskaia
D016954:Acinetobacter calcoaceticus / Q000378:metabolism*; D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000473:Alkanes / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D045424:Complex Mixtures / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D019327:Copper Sulfate / Q000378:metabolism / Q000494:pharmacology; D000431:Ethanol / Q000378:metabolism*; D005296:Ferrous Compounds / Q000378:metabolism / Q000494:pharmacology; D018086:Glutamate Dehydrogenase (NADP+) / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D006017:Glycolipids / Q000096:biosynthesis; D010730:Phosphoenolpyruvate Carboxylase / Q000378:metabolism; D017866:Phosphotransferases (Paired Acceptors) / Q000378:metabolism; D013501:Surface-Active Agents / Q000378:metabolism*; D015003:Yeasts / Q000737:chemistry; D019287:Zinc Sulfate / Q000378:metabolism / Q000494:pharmacology
D000473:Alkanes; D001426:Bacterial Proteins; D045424:Complex Mixtures; D005296:Ferrous Compounds; D006017:Glycolipids; D013501:Surface-Active Agents; C020748:ferrous sulfate; D000431:Ethanol; D019287:Zinc Sulfate; D000426:Alcohol Dehydrogenase; D018086:Glutamate Dehydrogenase (NADP+); D017866:Phosphotransferases (Paired Acceptors); C022509:pyruvate, water dikinase; D010730:Phosphoenolpyruvate Carboxylase; C007932:n-hexadecane; D019327:Copper Sulfate; D005990:Glycerol