Acinetobacter harbinensis sp. nov., isolated from river water.
A bacterial strain, HITLi 7T, with nitrifying ability was isolated from the surface water of the Songhua River in China. Cells were Gram-stain-negative, strictly aerobic, oxidase-negative, non-motile coccobacilli, capable of growth in mineral media with acetate as the sole carbon source and ammonia as the sole source of nitrogen. The cells did not grow at 37 °C, but did grow at 2 °C. The DNA G+C content was 45.5 mol%. Results of 16S rRNA gene sequence analysis indicated a close relationship between this isolate and Acinetobacter lwoffii (98.4% similarity for strain DSM 2403T). rpoB and gyrB gene sequences did not show significant similarity with those from other species of the genus Acinetobacter. Predominant cellular fatty acids were 9-octadecenoic acid (C18 : 1ω9c) and summed feature 4 (iso-C15:0 2-OH and/or C16:1ω7c). Acid was not produced from d-glucose, and gelatin was not hydrolysed by the isolate. Genotypic, phenotypic and chemotaxonomic data from this study indicate that the isolate should be classified as a representative of a novel species of the genus Acinetobacter. The name Acinetobacter harbinensis sp. nov. is proposed for the novel species, with HITLi 7T (=CGMCC 1.12528T=KCTC 32411T) as the type strain.
State Key Laboratory of Urban Water Resource and Environment, Harbin 150090, PR China.|School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, PR China.
Weiguang
W
Li
Department of Civil and Environmental Engineering, Aalto University, P.O. Box 15200, FI-00076 Aalto, Finland.|School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, PR China.
Duoying
D
Zhang
School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, PR China.
Xiaofei
X
Huang
School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, PR China.
Wen
W
Qin
D000150:Acinetobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology*; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S