Analysis of follicular fluid total phospholipids in women undergoing in-vitro fertilisation.
Follicular fluid (FF) samples were obtained from 100 patients referred to the University Hospital. A total of 79 subjects underwent IVF and the remaining 21 underwent ICSI. The levels of apoA-I and total phospholipid were measured using turbidometric and colorimetric phosphorus assays, respectively. Correlation analysis showed a significant inverse association of total phospholipid in FF with fertilisation ratio (r = -0.24, p = 0.04). Furthermore, the ratio of phospholipid/apoA-I in patients with a percentage of fertilised oocytes ≤ 50% was significantly higher (> 2.5%, p < 0.05) than in those with higher percentages of fertilised oocytes. The amounts of phospholipid and phospholipid/apoA-I ratio in FF were associated negatively to the percentage of oocyte fertilisation. Therefore, the change in the phospholipid and phospholipid/apoA-I ratio of FF might be regarded as indicators of female fertility.
Infertility and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences , Tehran , Iran.
S
S
Fayezi
M
M
Darabi
M
M
Darabi
M
M
Nouri
A
A
Rahimipour
A
A
Mehdizadeh
D000328:Adult; D016632:Apolipoprotein A-I / Q000378:metabolism*; D005260:Female; D005307:Fertilization in Vitro / Q000706:statistics & numerical data*; D015571:Follicular Fluid / Q000378:metabolism*; D006801:Humans; D007247:Infertility, Female / Q000378:metabolism*; D008297:Male; D010743:Phospholipids / Q000378:metabolism*; D011247:Pregnancy; D018873:Pregnancy Rate
D016632:Apolipoprotein A-I; D010743:Phospholipids