Irreversible site-specific hydrazinolysis of proteins by use of sortase.
Sortase-mediated hydrazinolysis of proteins with hydrazine or its derivatives was developed for the production of recombinant protein hydrazides. This process provides an alternative approach for protein semisynthesis through the use of recombinant protein hydrazides as thioester surrogates. It also provides an alternative method for C-terminal modification of proteins with functional units as well as for the preparation of C-to-C fusion proteins.
Tsinghua-Peking Center for Life Sciences, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084 (China); School of Medical Engineering, Hefei University of Technology, Hefei, Anhui 230009 (China).
Yi-Ming
YM
Li
Yi-Tong
YT
Li
Man
M
Pan
Xiu-Qi
XQ
Kong
Yi-Chao
YC
Huang
Zhang-Yong
ZY
Hong
Lei
L
Liu
D000595:Amino Acid Sequence; D019881:Aminoacyltransferases / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D019793:Fluorescein / Q000737:chemistry; D006834:Hydrazines / Q000737:chemistry*; D006868:Hydrolysis; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism*; D061905:Single-Domain Antibodies / Q000737:chemistry / Q000276:immunology
D001426:Bacterial Proteins; D006834:Hydrazines; D010455:Peptides; D011994:Recombinant Proteins; D061905:Single-Domain Antibodies; C029424:hydrazine; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases; D019793:Fluorescein
hydrazones; hydrolysis; ligation; protein modification; proteins