Rosiglitazone dampens pulmonary inflammation in a porcine model of acute lung injury.
The hallmarks of acute lung injury (ALI) are the compromised alveolar-capillary barrier and the extravasation of leukocytes into the alveolar space. Given the fact that the peroxisome proliferator-activated receptor-γ agonist rosiglitazone holds significant anti-inflammatory properties, we aimed to evaluate whether rosiglitazone could dampen these hallmarks of local pulmonary inflammation in a porcine model of lung injury. For this purpose, we used a model of lipopolysaccharide (LPS, 50 μg/kg)-induced ALI. One hundred twenty minutes following the infusion of LPS, we started the exposure to rosiglitazone through inhalation or infusion. We found that intravenous rosiglitazone significantly controlled local pulmonary inflammation as determined through the expression of cytokines within the alveolar compartment. Furthermore, we found a significant reduction of the protein concentration and neutrophil activity within the alveolar space. In summary, we therefore conclude that the treatment with rosiglitazone might dampen local pulmonary inflammation during the initial stages of ALI.
Department of Anesthesiology and Intensive Care Medicine, Tübingen University Hospital, Eberhard-Karls University, Tübingen, Germany.
Valbona
V
Mirakaj
Christian
C
Mutz
Dierk
D
Vagts
Janek
J
Henes
Helene A
HA
Haeberle
Susanne
S
Husung
Tony
T
König
Gabriele
G
Nöldge-Schomburg
Peter
P
Rosenberger
D055371:Acute Lung Injury / Q000188:drug therapy*; D000280:Administration, Inhalation; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D001999:Bronchoscopy; D002404:Catheterization; D004195:Disease Models, Animal; D004731:Endotoxins / Q000737:chemistry; D006439:Hemodynamics; D007004:Hypoglycemic Agents / Q000627:therapeutic use; D007249:Inflammation / Q000188:drug therapy*; D007262:Infusions, Intravenous; D008070:Lipopolysaccharides; D008168:Lung / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000378:metabolism; D000077154:Rosiglitazone; D013552:Swine; D045162:Thiazolidinediones / Q000627:therapeutic use*
D004731:Endotoxins; D007004:Hypoglycemic Agents; D008070:Lipopolysaccharides; D045162:Thiazolidinediones; D000077154:Rosiglitazone; D009195:Peroxidase