Cellular behavior of human adipose-derived stem cells on wettable gradient polyethylene surfaces.
Appropriate surface wettability and roughness of biomaterials is an important factor in cell attachment and proliferation. In this study, we investigated the correlation between surface wettability and roughness, and biological response in human adipose-derived stem cells (hADSCs). We prepared wettable and rough gradient polyethylene (PE) surfaces by increasing the power of a radio frequency corona discharge apparatus with knife-type electrodes over a moving sample bed. The PE changed gradually from hydrophobic and smooth surfaces to hydrophilic (water contact angle, 90° to ~ 50°) and rough (80 to ~120 nm) surfaces as the power increased. We found that hADSCs adhered better to highly hydrophilic and rough surfaces and showed broadly stretched morphology compared with that on hydrophobic and smooth surfaces. The proliferation of hADSCs on hydrophilic and rough surfaces was also higher than that on hydrophobic and smooth surfaces. Furthermore, integrin beta 1 gene expression, an indicator of attachment, and heat shock protein 70 gene expression were high on hydrophobic and smooth surfaces. These results indicate that the cellular behavior of hADSCs on gradient surface depends on surface properties, wettability and roughness.
Department of Neurosurgery, Catholic University of Korea, Daejeon 301-723, Korea. windy818@hanmail.net.
Hyun Hee
HH
Ahn
Department of Neurosurgery, Catholic University of Korea, Daejeon 301-723, Korea. leeilwoo@catholic.ac.kr.
Il Woo
IW
Lee
Department of Molecular Science and Technology, Ajou University, Suwon 443-759, Korea. hblee@ajou.ac.kr.
Hai Bang
HB
Lee
Department of Molecular Science and Technology, Ajou University, Suwon 443-759, Korea. moonskim@ajou.ac.kr.
Moon Suk
MS
Kim
D000273:Adipose Tissue / Q000166:cytology*; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D005260:Female; D015870:Gene Expression; D050884:HSP72 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D019012:Integrin beta1 / Q000235:genetics / Q000378:metabolism; D011095:Polyethylenes / Q000737:chemistry; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D013499:Surface Properties; D017155:Wettability
D050884:HSP72 Heat-Shock Proteins; D019012:Integrin beta1; D011095:Polyethylenes