Role of Acorus calamus and alpha-asarone on hippocampal dependent memory in noise stress exposed rats.
Stress is a condition or stimulus that threatens an organism's survival. Noise is an environmental stressor. It is well known that long term as well as acute exposure to noise led to oxidative stress. In the present study, it was investigated that the persistence of noise stress (100 dBA/4 h/d for 30 days) could cause memory impairment in rats and whether ethylacetate extract of AC EAAC (50 mg kg(-1) b.wt.) and alpha-Asarone (9 mg kg(-1) b.wt.). treatment can prevent or not. In order to understand the possible mechanism behind it, antioxidant status and acetylcholinesterase (AChE) activity in hippocampus was evaluated after rats were tested in Radial Eight-arm Maze (RAM). Heat shock protein 70 (hsp 70) expression in hippocampus was also evaluated to understand the intensity of stress level. Results showed that after noise stress exposure, time taken to visit all the baited arms, working and reference memory errors were increased in RAM. The superoxide dismutase, lipid peroxidation, AChE activity, hsp 70 were significantly increased with concomitant decrease in catalase, glutathione peroxidase activity and G6PD activity of non-enzymatic levels was observed in the 30 days noise stress exposed group. When rats were co-administrated with EAAC and alpha-Asarone prevents the noise stress induced alterations significantly. In Conclusion, noise stress induced oxidative stress, increased AChE activity, and over expression of hsp 70 in hippocampus region might have led to the impairment of spatial memory. EAAC and alpha-Asarone prevents this noise stress induced memory impairment.
Department of Physiology, Tagore Medical College and Hospital, Chennai, India.
Manikandan
M
Sundaramahalingam
Sri Lakshmi Narayana Institute of Medical Sciences, Pondicherry, India.
Srikumar
S
Ramasundaram
Department of Physiology, Dr. ALM. PG. Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai-600 113, India.
Sheela Devi
SD
Rathinasamy
Department of Physiology, Tagore Medical College and Hospital, Chennai, India.
Ruvanthika Pulipakkam
RP
Natarajan
Department of Physiology, Tagore Medical College and Hospital, Chennai, India.
Thangam
T
Somasundaram
D000110:Acetylcholinesterase / Q000378:metabolism; D029023:Acorus; D000081248:Allylbenzene Derivatives; D000818:Animals; D000840:Anisoles / Q000494:pharmacology*; D004195:Disease Models, Animal; D058851:GPI-Linked Proteins / Q000378:metabolism; D050884:HSP72 Heat-Shock Proteins / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008568:Memory / Q000187:drug effects*; D008569:Memory Disorders / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology / Q000523:psychology; D009043:Motor Activity / Q000187:drug effects; D009622:Noise / Q000009:adverse effects*; D018697:Nootropic Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D051381:Rats; D017208:Rats, Wistar; D013315:Stress, Psychological / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology / Q000523:psychology; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors
D000081248:Allylbenzene Derivatives; D000840:Anisoles; D058851:GPI-Linked Proteins; D050884:HSP72 Heat-Shock Proteins; D018697:Nootropic Agents; D010936:Plant Extracts; C012195:asarone; D013482:Superoxide Dismutase; D000110:Acetylcholinesterase; C548246:Ache protein, rat