Contact angle changes induced by immunocomplex formation.
Immunoassays analyzing interactions between antigens and antibodies can be affected by capillary action together with binding affinity. This paper studies contact-angle changes of bacterial suspensions on antibody immobilized surfaces. The capillary action and the dried pattern of the bacterial suspensions are analyzed and correlated with specific- and nonspecific bindings between bacteria and antibodies.
Department of Mechanical Engineering, University of Washington, Seattle, WA, USA. jae71@uw.edu.
Jong-Hoon
JH
Kim
Amy Q
AQ
Shen
Kyong-Hoon
KH
Lee
Gerard A
GA
Cangelosi
Jae-Hyun
JH
Chung
D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry*; D000936:Antigen-Antibody Complex / Q000276:immunology*; D002417:Cattle; D004867:Equipment Design; D004926:Escherichia coli / Q000276:immunology / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology; D007118:Immunoassay / Q000295:instrumentation*; D009163:Mycobacterium bovis / Q000276:immunology / Q000302:isolation & purification*; D014380:Tuberculosis, Bovine / Q000382:microbiology
D055910:Antibodies, Immobilized; D000936:Antigen-Antibody Complex