Calorie restriction upregulated sirtuin 1 by attenuating its ubiquitin degradation in cancer cells.
Sirtuin (SIRT) 1 is a key protein in mediating the benefits of calorie restriction (CR) in mammals. However, the molecular mechanisms underlying CR-induced SIRT1 upregulation in mammals remain unclear. Herein we show that the elevated SIRT1 levels are not due to increased SIRT1 mRNA expression. but rather to enhanced SIRT1 protein stability as a result of reduced ubiquitin-proteasome degradation of SIRT1 under limited nutrient conditions. Our observations have important implications for improving healthy aging in humans.
Peking University Research Center on Ageing, Peking University Health Science Center, Beijing, China; Department of Biochemistry & Molecular Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
Limin
L
Han
Ganye
G
Zhao
Hui
H
Wang
Tanjun
T
Tong
Jun
J
Chen
D031204:Caloric Restriction / Q000379:methods; D045744:Cell Line, Tumor; D006367:HeLa Cells; D006801:Humans; D046988:Proteasome Endopeptidase Complex / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D056564:Sirtuin 1 / Q000235:genetics* / Q000378:metabolism*; D025801:Ubiquitin / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000235:genetics*
D012333:RNA, Messenger; D025801:Ubiquitin; D046988:Proteasome Endopeptidase Complex; C447939:SIRT1 protein, human; D056564:Sirtuin 1
calorie restriction; sirtuin 1; ubiquitination