Nanoclustering and heterogeneous membrane diffusion of Ras studied by FRAP and RICS analysis.
Fluorescence Recovery After Photobleaching (FRAP) and Raster Image Correlation Spectroscopy (RICS) are two powerful techniques to study the diffusion dynamics of fluorescently labeled proteins. FRAP and RICS can be easily applied on any commercial confocal microscope. In this chapter, we describe the principles of these methods and provide the reader with a detailed guide on how to apply these methods in the study of Ras nanoclustering and diffusion in the plasma membrane of live cells.
Turku Centre for Biotechnology, Åbo Akademi University, Turku, Finland.
Camilo
C
Guzmán
Maja
M
Solman
Daniel
D
Abankwa
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D006224:Cricetinae; D004058:Diffusion; D036681:Fluorescence Recovery After Photobleaching / Q000379:methods*; D018613:Microscopy, Confocal; D049329:Nanostructures; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013223:Statistics as Topic; D018631:ras Proteins / Q000737:chemistry* / Q000378:metabolism*
D018631:ras Proteins