Hesperidin, a citrus bioflavonoid, ameliorates genotoxicity-induced by diazinon in human blood lymphocytes.
Hesperidin (Hes), a natural bioflavonoid, is abundant in citrus fruit and has been reported to exert a wide range of pharmacological effects. Diazinon (DZN) can be mutagenic, or capable of inducing genetic damage, in human blood cells. The protective effect of Hes against DZN-induced micronucleus formation, an index of DNA damage, was investigated in human blood lymphocytes. Whole blood samples were collected from 5 volunteers and were incubated with different Hes concentrations for 3 h. The samples were then incubated with 750 µM DZN for 24 h. Subsequently, the blood samples were cultured with a mitogenic stimulant to evaluate micronucleus formation in cytokinesis-blocked binucleated lymphocytes. The incubation of blood samples with DZN induced additional genotoxicity in lymphocytes, and Hes pretreatment significantly reduced the micronucleus frequency (p<0.01-p<0.001). Hes revealed a potent antigenotoxic effect against DZN-induced DNA damage, which may be due to free radical scavenging property. Since hesperidin is a natural compound and is considered safe, it can be used as a supplement to protect people exposed to chemical or environmental hazards.
Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
M
M
Shokrzadeh
Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
A
A
Ahmadi
Student Research Committee, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
S
S
Ramezaninejhad
Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
A
A
Shadboorestan
D000328:Adult; D002478:Cells, Cultured; D004249:DNA Damage / Q000187:drug effects*; D003976:Diazinon / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D006569:Hesperidin / Q000494:pharmacology*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects
D006569:Hesperidin; D003976:Diazinon