β-Conglycinin reduces the tight junction occludin and ZO-1 expression in IPEC-J2.
Soybean allergy presents a health threat to humans and animals. The mechanism by which food/feed allergen β-conglycinin injures the intestinal barrier has not been well understood. In this study, the changes of epithelial permeability, integrity, metabolic activity, the tight junction (TJ) distribution and expression induced by β-conglycinin were evaluated using IPEC-J2 model. The results showed a significant decrease of trans-epithelial electrical resistance (TEER) (p < 0.001) and metabolic activity (p < 0.001) and a remarkable increase of alkaline phosphatase (AP) activity (p < 0.001) in a dose-dependent manner. The expression levels of tight junction occludin and ZO-1 were decreased (p < 0.05). The reduced fluorescence of targets and change of cellular morphology were recorded. The tight junction occludin and ZO-1 mRNA expression linearly declined with increasing β-conglycinin (p < 0.001).
College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China. zhaoyuan4CL52@126.com.
Yuan
Y
Zhao
College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China. qgx@jlau.edu.cn.
Guixin
G
Qin
College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China. hanrui0409@163.com.
Rui
R
Han
College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China. wangjun4169@126.com.
Jun
J
Wang
Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China. zhang_xd@jlu.edu.cn.
Xiaodong
X
Zhang
College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China. ddliu19880922@163.com.
Dandan
D
Liu
D000818:Animals; D052179:Antigens, Plant / Q000494:pharmacology*; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects*; D005916:Globulins / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D062793:Occludin / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000494:pharmacology; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics; D055314:Seed Storage Proteins / Q000494:pharmacology*; D030262:Soybean Proteins / Q000494:pharmacology*; D013552:Swine; D019108:Tight Junctions / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D062826:Zonula Occludens-1 Protein / Q000235:genetics* / Q000378:metabolism
D052179:Antigens, Plant; D005916:Globulins; D062793:Occludin; D010940:Plant Proteins; D012333:RNA, Messenger; D055314:Seed Storage Proteins; D030262:Soybean Proteins; D062826:Zonula Occludens-1 Protein; C013894:beta-conglycinin protein, Glycine max