Identification and molecular characterization of a chitin deacetylase from Bombyx mori peritrophic membrane.
The insect midgut epithelium is generally lined with a unique chitin and protein structure, the peritrophic membrane (PM), which facilitates food digestion and protects the gut epithelium. PM proteins are important determinants for PM structure and formation. In this study, the silkworm Bombyx mori midgut PM protein BmCDA7 was identified by proteomic tools. The full-length BmCDA7 cDNA is 1357 bp; the deduced protein is composed of 379 amino acid residues and includes a 16 amino acid residue signal peptide, a putative polysaccharide deacetylase-like domain and 15 cysteine residues present in three clusters. The heterologously expressed proteins of the BmCDA7 gene in yeast displayed chitin deacetylase activity. Expression of B. mori BmCDA7 was detected in the midgut at both the transcriptional and translational levels. The BmCDA7 gene was expressed by the newly hatched silkworm larvae until day seven of the fifth instar and was expressed at a high level in the newly exuviated larvae of different instars. The functions and regulatory mechanism of BmCDA7, however, need further investigation.
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China. zxw_strive@163.com.
Xiao-Wu
XW
Zhong
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China. wangxiaohuan1024@126.com.
Xiao-Huan
XH
Wang
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China. luffy.tan@gmail.com.
Xiang
X
Tan
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China. xiaqy@swu.edu.cn.
Qing-You
QY
Xia
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China. xbxzh@swu.edu.cn.
Zhong-Huai
ZH
Xiang
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China. zhaop@swu.edu.cn.
Ping
P
Zhao
D000581:Amidohydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000201:enzymology* / Q000235:genetics*; D004789:Enzyme Activation; D015870:Gene Expression; D019476:Insect Proteins; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D021381:Protein Transport; D040901:Proteomics; D011994:Recombinant Proteins
D019476:Insect Proteins; D011994:Recombinant Proteins; D000581:Amidohydrolases; C019866:chitin deacetylase