Transcription of the SCL/TAL1 interrupting Locus (Stil) is required for cell proliferation in adult Zebrafish Retinas.
The human oncogene SCL/TAL1 interrupting locus (Stil) is highly conserved in vertebrate species. Previously, we identified a homolog of the Stil gene in zebrafish mutant (night blindness b, nbb), which showed neural defects in the retina (e.g. dopaminergic cell degeneration and/or lack of regeneration). In this research, we examined the roles of Stil in cell proliferation after degeneration in adult zebrafish retinas. We demonstrated that knockdown of Stil gene expression or inhibition of Sonic hedgehog (Shh) signaling transduction decreases the rate of cell proliferation. In contrast, activation of Shh signal transduction promotes cell proliferation. In nbb(+/-) retinas, inhibition of SUFU (a repressor in the Shh pathway) rescues the defects in cell proliferation due to down-regulation of Stil gene expression. The latter data suggest that Stil play a role in cell proliferation through the Shh signal transduction pathway.
Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana 46556; Department of Physiology, Nankai University, Tianjin, China 370001.
Lei
L
Sun
Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana 46556.
Ping
P
Li
Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana 46556; Center for Zebrafish Research, University of Notre Dame, Notre Dame, Indiana 46556.
Aprell L
AL
Carr
Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana 46556; Center for Zebrafish Research, University of Notre Dame, Notre Dame, Indiana 46556.
Ryne
R
Gorsuch
Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana 46556.
Clare
C
Yarka
Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana 46556; Center for Zebrafish Research, University of Notre Dame, Notre Dame, Indiana 46556.
Jingling
J
Li
Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana 46556.
Michael
M
Bartlett
Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana 46556.
Delaney
D
Pfister
Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana 46556; Center for Zebrafish Research, University of Notre Dame, Notre Dame, Indiana 46556.
David R
DR
Hyde
Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana 46556; Center for Zebrafish Research, University of Notre Dame, Notre Dame, Indiana 46556. Electronic address: Li.78@nd.edu.
Lei
L
Li
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000502:physiology*; D049109:Cell Proliferation; D063928:Ependymoglial Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D053823:Hedgehog Proteins / Q000235:genetics / Q000378:metabolism*; D016627:Oxidopamine / Q000494:pharmacology; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000502:physiology*; D012097:Repressor Proteins / Q000235:genetics; D012160:Retina / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D012162:Retinal Degeneration / Q000139:chemically induced / Q000473:pathology; D017948:Retinal Rod Photoreceptor Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D015398:Signal Transduction; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; D015027:Zebrafish / Q000235:genetics / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*
D051792:Basic Helix-Loop-Helix Proteins; D053823:Hedgehog Proteins; D016627:Oxidopamine; D011518:Proto-Oncogene Proteins; D012097:Repressor Proteins; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; D029961:Zebrafish Proteins; C524053:Shha protein, zebrafish; C518960:Sufu protein, zebrafish; C489367:tal1 protein, zebrafish
Cell Proliferation; Neurons; Oncogene; Retina; Zebrafish