Drought stress-induced compositional changes in tolerant transgenic rice and its wild type.
Comparing well-watered versus deficit conditions, we evaluated the chemical composition of grains harvested from wild-type (WT) and drought-tolerant, transgenic rice (Oryza sativa L.). The latter had been developed by inserting AtCYP78A7, which encodes a cytochrome P450 protein. Two transgenic Lines, '10B-5' and '18A-4', and the 'Hwayoung' WT were grown under a rainout shelter. After the harvested grains were polished, their levels of key components, including proximates, amino acids, fatty acids, minerals and vitamins were analysed to determine the effect of watering system and genotype. Drought treatment significantly influenced the levels of some nutritional components in both transgenic and WT grains. In particular, the amounts of lignoceric acid and copper in the WT decreased by 12.6% and 39.5%, respectively, by drought stress, whereas those of copper and potassium in the transgenics rose by 88.1-113.3% and 10.4-11.9%, respectively, under water-deficit conditions.
Bio-Evaluation Center, KRIBB, 30 Yeongudanji-ro, Ochang-eup, Cheongwon, 363-883, Republic of Korea.
Kyong-Hee
KH
Nam
Bio-Evaluation Center, KRIBB, 30 Yeongudanji-ro, Ochang-eup, Cheongwon, 363-883, Republic of Korea.
Do-Young
DY
Kim
Bio-Evaluation Center, KRIBB, 30 Yeongudanji-ro, Ochang-eup, Cheongwon, 363-883, Republic of Korea.
Hee Jae
HJ
Shin
Bio-Evaluation Center, KRIBB, 30 Yeongudanji-ro, Ochang-eup, Cheongwon, 363-883, Republic of Korea.
Ki Jung
KJ
Nam
Bio-Evaluation Center, KRIBB, 30 Yeongudanji-ro, Ochang-eup, Cheongwon, 363-883, Republic of Korea.
Joo Hee
JH
An
Bio-Evaluation Center, KRIBB, 30 Yeongudanji-ro, Ochang-eup, Cheongwon, 363-883, Republic of Korea.
In-Soon
IS
Pack
Bio-Evaluation Center, KRIBB, 30 Yeongudanji-ro, Ochang-eup, Cheongwon, 363-883, Republic of Korea.
Jung-Ho
JH
Park
Bio-Evaluation Center, KRIBB, 30 Yeongudanji-ro, Ochang-eup, Cheongwon, 363-883, Republic of Korea.
Soon-Chun
SC
Jeong
Life Sciences Research Institute, Biomedic Co., Ltd., Bucheon 420-020, Republic of Korea.
Ho Bang
HB
Kim
Bio-Evaluation Center, KRIBB, 30 Yeongudanji-ro, Ochang-eup, Cheongwon, 363-883, Republic of Korea. Electronic address: cgkim@kribb.re.kr.
Chang-Gi
CG
Kim
D000596:Amino Acids / Q000032:analysis; D055864:Droughts; D005227:Fatty Acids / Q000032:analysis; D005838:Genotype; D008903:Minerals / Q000032:analysis; D012275:Oryza / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D013312:Stress, Physiological; D014815:Vitamins / Q000032:analysis; D014867:Water / Q000378:metabolism
D000596:Amino Acids; D005227:Fatty Acids; D008903:Minerals; D014815:Vitamins; D014867:Water
Chemical composition; Drought tolerance; Food safety assessment; Oryza sativa L.; Rainout shelter; Transgenic crop