Effect of bacteriophage on the susceptibility, motility, invasion, and survival of Salmonella Typhimurium exposed to the simulated intestinal conditions.
This study was designed to evaluate the effect of bacteriophage P22 on the susceptibility, swimming motility, invasion gene expression, invasive ability, and intracellular survival of Salmonella Typhimurium exposed to the simulated intestinal conditions. S. Typhimurium cells were inoculated at 37 °C for 4 h in the simulated intestinal conditions with or without bacteriophage P22, including control (0 % bile salts, pH 7.2), SN (0 % bile salts, pH 5.0), SL (0.5 % bile salts, pH 5.0), SH (2.0 % bile salts, pH 5.0), SNp (0 % bile salts + P22, pH 5.0), SLp (0.5 % bile salts + P22, pH 5.0), and SHp (2.0 % bile salts + P22, pH 5.0). The numbers of Typhimurium cells were significantly reduced by 3.30, 3.56, and 3.75 log units, respectively, at SNp, SLp, and SHp. Considerable reduction in the swimming motility was observed at SNp (23 %), SLp (22 %), and SHp (20 %). The transcriptional regulator genes, hilA, hilC, hilD, invA, invE, and invF, were significantly down-regulated with SHp, showing 4.07-fold, 2.87-fold, 3.43-fold, 2.07-fold, 1.44-fold, and 4.83-fold, respectively. The decrease in invasive ability was most significant at SHp (45 %), followed by SLp (49 %). These results suggest that bacteriophage P22 can be used as an alternative to control Salmonella invasion of epithelial cells.
Department of Medical Biomaterials Engineering, Kangwon National University, Chuncheon, 200-701, Gangwon, Republic of Korea.
Kanghee
K
Ryu
Debabrata
D
Biswas
Juhee
J
Ahn
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001435:Bacteriophages / Q000502:physiology*; D002460:Cell Line; D004847:Epithelial Cells / Q000382:microbiology*; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D050296:Microbial Viability; D012486:Salmonella typhimurium / Q000235:genetics / Q000502:physiology* / Q000821:virology*
D001426:Bacterial Proteins