Identification of germline transcriptional regulatory elements in Aedes aegypti.
The mosquito Aedes aegypti is the principal vector for the yellow fever and dengue viruses, and is also responsible for recent outbreaks of the alphavirus chikungunya. Vector control strategies utilizing engineered gene drive systems are being developed as a means of replacing wild, pathogen transmitting mosquitoes with individuals refractory to disease transmission, or bringing about population suppression. Several of these systems, including Medea, UD(MEL), and site-specific nucleases, which can be used to drive genes into populations or bring about population suppression, utilize transcriptional regulatory elements that drive germline-specific expression. Here we report the identification of multiple regulatory elements able to drive gene expression specifically in the female germline, or in the male and female germline, in the mosquito Aedes aegypti. These elements can also be used as tools with which to probe the roles of specific genes in germline function and in the early embryo, through overexpression or RNA interference.
1] Division of Biology, MC 156-29, California Institute of Technology, Pasadena, CA 91125, USA [2].
Omar S
OS
Akbari
1] Division of Biology, MC 156-29, California Institute of Technology, Pasadena, CA 91125, USA [2].
Philippos A
PA
Papathanos
Division of Biology, MC 156-29, California Institute of Technology, Pasadena, CA 91125, USA.
Jeremy E
JE
Sandler
Division of Biology, MC 156-29, California Institute of Technology, Pasadena, CA 91125, USA.
Katie
K
Kennedy
Division of Biology, MC 156-29, California Institute of Technology, Pasadena, CA 91125, USA.
Bruce A
BA
Hay
D000330:Aedes / Q000235:genetics* / Q000821:virology; D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics; D065632:Chikungunya Fever / Q000635:transmission / Q000821:virology; D003715:Dengue / Q000635:transmission / Q000821:virology; D029721:Drosophila Proteins / Q000235:genetics; D005260:Female; D015870:Gene Expression; D017344:Genes, Insect / Q000235:genetics*; D049452:Green Fluorescent Proteins; D007303:Insect Vectors / Q000235:genetics* / Q000821:virology; D008297:Male; D011401:Promoter Regions, Genetic / Q000235:genetics; D050436:Regulatory Elements, Transcriptional / Q000235:genetics*; D051901:Smad4 Protein / Q000235:genetics; D015004:Yellow Fever / Q000635:transmission / Q000821:virology
D029721:Drosophila Proteins; C112504:Med protein, Drosophila; D051901:Smad4 Protein; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins