Aedes aegypti midgut remodeling during metamorphosis.
The Aedes aegypti midgut is restructured during metamorphosis; its epithelium is renewed by replacing the digestive and endocrine cells through stem or regenerative cell differentiation. Shortly after pupation (white pupae) begins, the larval digestive cells are histolized and show signs of degeneration, such as autophagic vacuoles and disintegrating microvilli. Simultaneously, differentiating cells derived from larval stem cells form an electron-dense layer that is visible 24h after pupation begins. Forty-eight hours after pupation onset, the differentiating cells yield an electron-lucent cytoplasm rich in microvilli and organelles. Dividing stem cells were observed in the fourth instar larvae and during the first 24h of pupation, which suggests that stem cells proliferate at the end of the larval period and during pupation. This study discusses various aspects of the changes during midgut remodeling for pupating A. aegypti.
Programa de Pós-graduação em Biologia Celular e Estrutural, Departamento de Biologia Geral, Universidade Federal de Viçosa (DBG/UFV), Campus Universitário, Viçosa, Minas Gerais, CEP 36570-900, Brazil. Electronic address: kennerbio@yahoo.com.br.
Kenner Morais
KM
Fernandes
Programa de Pós-graduação em Biologia Celular e Estrutural, Departamento de Biologia Geral, Universidade Federal de Viçosa (DBG/UFV), Campus Universitário, Viçosa, Minas Gerais, CEP 36570-900, Brazil. Electronic address: caneves@ufv.br.
Clóvis Andrade
CA
Neves
Programa de Pós-graduação em Biologia Celular e Estrutural, Departamento de Biologia Geral, Universidade Federal de Viçosa (DBG/UFV), Campus Universitário, Viçosa, Minas Gerais, CEP 36570-900, Brazil. Electronic address: jeserrao@ufv.br.
José Eduardo
JE
Serrão
Programa de Pós-graduação em Biologia Celular e Estrutural, Departamento de Biologia Geral, Universidade Federal de Viçosa (DBG/UFV), Campus Universitário, Viçosa, Minas Gerais, CEP 36570-900, Brazil. Electronic address: gmartins@ufv.br.
Gustavo Ferreira
GF
Martins
D000330:Aedes / Q000254:growth & development* / Q000648:ultrastructure*; D000818:Animals; D005260:Female; D041981:Gastrointestinal Tract / Q000254:growth & development / Q000648:ultrastructure; D007814:Larva / Q000254:growth & development / Q000648:ultrastructure; D008675:Metamorphosis, Biological; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D011679:Pupa / Q000254:growth & development / Q000648:ultrastructure
Aedes aegypti; Development; Endocrine cells; Midgut; Regenerative cells; Stem cells