Prenatal exposure to lipopolysaccharide combined with pre- and postnatal high-fat diet result in lowered blood pressure and insulin resistance in offspring rats.
BACKGROUND
Adult metabolic syndrome may in part have origins in fetal or early life. This study was designed to explore the effect of prenatal exposure to lipopolysaccharide and high-fat diet on metabolic syndrome in offspring rats.


METHODS
32 pregnant rats were randomly divided into four groups, including Control group; LPS group (pregnant rats were injected with LPS 0.4 mg/kg intraperitoneally on the 8(th), 10(th) and 12(th) day of pregnancy); High-fat group (maternal rats had high-fat diet during pregnancy and lactation period, and their pups also had high-fat diet up to the third month of life); LPS + High-fat group (rats were exposed to the identical experimental scheme with LPS group and High-fat group).


RESULTS
Blood pressure elevated in LPS group and High-fat group, reduced in LPS+High-fat group, accompanied by the increase of serum leptin level in LPS and High-fat group and increase of serum IL-6, TNF-a in High-fat group; both serum insulin and cholesterol increased in High-fat and LPS+High-fat group, as well as insulin in LPS group. HOMA-IR value increased in LPS, High-fat and LPS+High-fat group, and QUICKI decreased in these groups; H-E staining showed morphologically pathological changes in thoracic aorta and liver tissue in the three groups. Increased serum alanine and aspartate aminotransferase suggest impaired liver function in LPS+High-fat group.


CONCLUSIONS
Prenatal exposure to lipopolysaccharide combined with pre- and postnatal high-fat diet result in lowered blood pressure, insulin resistance and impaired liver function in three-month old offspring rats. The lowered blood pressure might benefit from the predictive adaptive response to prenatal inflammation.
Department of Pharmacy, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, PR China.
Xue-Qin
XQ
Hao
Department of pharmacology, Medical College, Henan University of Science and Technology, Luoyang, PR China.
Jing-Xia
JX
Du
Department of pharmacology, Medical College, Henan University of Science and Technology, Luoyang, PR China.
Yan
Y
Li
Luoyang Entry-Exit Inspection and Quarantine Bureau, Luoyang, PR China.
Meng
M
Li
Department of Cardiology, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, PR China.
Shou-Yan
SY
Zhang
D000818:Animals; D000831:Animals, Newborn; D001786:Blood Glucose / Q000378:metabolism; D001794:Blood Pressure / Q000187:drug effects*; D001835:Body Weight; D002784:Cholesterol / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007328:Insulin / Q000378:metabolism; D007333:Insulin Resistance; D007677:Kidney Function Tests; D008070:Lipopolysaccharides / Q000009:adverse effects*; D008111:Liver Function Tests; D008297:Male; D024821:Metabolic Syndrome / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000209:etiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014280:Triglycerides / Q000378:metabolism
D001786:Blood Glucose; D016207:Cytokines; D007328:Insulin; D008070:Lipopolysaccharides; D014280:Triglycerides; D002784:Cholesterol