Investigations of an electrochemical platform based on the layered MoS2-graphene and horseradish peroxidase nanocomposite for direct electrochemistry and electrocatalysis.
The self-assembly of layered molybdenum disulfide-graphene (MoS2-Gr) and horseradish peroxidase (HRP) by electrostatic attraction into a novel hybrid nanomaterial (HRP-MoS2-Gr) is reported. The properties of the MoS2-Gr were characterized by X-ray diffraction (XRD), high-resolution transmission electron microscopy (TEM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). UV-vis and Fourier transform infrared spectroscopy (FT-IR) indicate that the native structure of the HRP is maintained after the assembly, implying good biocompatibility of MoS2-Gr nanocomposite. Furthermore, the HRP-MoS2-Gr composite is utilized as a biosensor, which displays electrocatalytic activity to hydrogen peroxide (H2O2) with high sensitivity (679.7 μA mM(-1)cm(-2)), wide linear range (0.2 μM-1.103 mM), low detection limit (0.049 μM), and fast amperometric response. In addition, the biosensor also exhibits strong anti-interference ability, satisfactory stability and reproducibility. These desirable electrochemical properties are attributed to the good biocompatibility and electron transport efficiency of the MoS2-Gr composite, as well as the high loading of HRP. Therefore, this biosensor is potentially suitable for H2O2 analysis in environmental, pharmaceutical, food or industrial applications.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; Department of Chemistry, Nanchang University, Nanchang 330031, China.
Haiyan
H
Song
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; Department of Chemistry, Nanchang University, Nanchang 330031, China. Electronic address: ynni@ncu.edu.cn.
Yongnian
Y
Ni
School of Chemistry, Physics and Mechanical Engineering, Queensland University of Technology, Brisbane 4001, Australia. Electronic address: s.kokot@qut.edu.au.
Serge
S
Kokot
D015374:Biosensing Techniques / Q000295:instrumentation*; D004220:Disulfides / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D006108:Graphite / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000378:metabolism; D057230:Limit of Detection; D008982:Molybdenum / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
D004220:Disulfides; D004800:Enzymes, Immobilized; D006108:Graphite; D008982:Molybdenum; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; C082964:molybdenum disulfide
Electrocatalysis; Horseradish peroxidase; Hydrogen peroxide; Layered nanocomposites; MoS(2)–graphene