Whole genomic sequence and replication kinetics of a new enterovirus C96 isolated from Guangdong, China with a different cell tropism.
Enterovirus 96 (EV-C96) is a newly described serotype within the enterovirus C (EV-C) species, and its biological and pathological characters are largely unknown. In this study, we sequenced the whole genome of a novel EV-C96 strain that was isolated in 2011 from a patient with acute flaccid paralysis (AFP) in Guangdong province, China and characterized the properties of its infection. Sequence analysis revealed the close relationship between the EV-C96 strains isolated from the Guangdong and Shandong provinces of China, and suggested that recombination events occurred both between these EV-C96 strains and with other EV-C viruses. Moreover, the virus replication kinetics showed EV-C96 Guangdong strain replicated at a high rate in RD cells and presented a different cell tropism to other strains isolated from Shandong recently. These findings gave further insight into the evolutionary processes and extensive biodiversity of EV-C96.
Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China ; Guangdong Provincial Institution of Public Health, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China.
Jing
J
Lu
Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China.
Huanying
H
Zheng
WHO WPRO Regional Polio Reference Laboratory and Ministry of Health Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Yong
Y
Zhang
Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China.
Xue
X
Guo
Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China.
De
D
Wu
Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China.
Hui
H
Li
Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China.
Leng
L
Liu
Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China.
Hanri
H
Zeng
Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China ; Guangdong Provincial Institution of Public Health, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China.
Lina
L
Yi
Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China.
Ling
L
Fang
Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China.
Yanling
Y
Mo
WHO WPRO Regional Polio Reference Laboratory and Ministry of Health Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Wenbo
W
Xu
Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China.
Changwen
C
Ke
D000818:Animals; D045744:Cell Line, Tumor; D002675:Child, Preschool; D002681:China; D002522:Chlorocebus aethiops; D030015:Enterovirus C, Human / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D004769:Enterovirus Infections / Q000821:virology*; D005260:Female; D016679:Genome, Viral; D006801:Humans; D007700:Kinetics; D010264:Paraplegia / Q000821:virology*; D010802:Phylogeny; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D014709:Vero Cells; D015678:Viral Structural Proteins / Q000235:genetics; D014779:Virus Replication
D015678:Viral Structural Proteins