Isolation and characterization of a dominant dwarf gene, d-h, in rice.
Plant height is an important agronomic trait that affects grain yield. Previously, we reported a novel semi-dominant dwarf mutant, HD1, derived from chemical mutagenesis using N-methyl-N-nitrosourea (MNU) on a japonica rice cultivar, Hwacheong. In this study, we cloned the gene responsible for the dwarf mutant using a map-based approach. Fine mapping revealed that the mutant gene was located on the short arm of chromosome 1 in a 48 kb region. Sequencing of the candidate genes and rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR) analysis identified the gene, d-h, which encodes a protein of unknown function but whose sequence is conserved in other cereal crops. Real-time (RT)-PCR analysis and promoter activity assays showed that the d-h gene was primarily expressed in the nodes and the panicle. In the HD1 plant, the d-h gene was found to carry a 63-bp deletion in the ORF region that was subsequently confirmed by transgenic experiments to be directly responsible for the gain-of-function phenotype observed in the mutant. Since the mutant plants exhibit a defect in GA response, but not in the GA synthetic pathway, it appears that the d-h gene may be involved in a GA signaling pathway.
Department of Plant Science, Research Institute of Agriculture and Life Sciences and Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul, Korea.
Rihua
R
Piao
Department of Plant Science, Research Institute of Agriculture and Life Sciences and Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul, Korea.
Sang-Ho
SH
Chu
College of Plant Science, Jilin University, Changchun, China.
Wenzhu
W
Jiang
Department of Plant Science, Research Institute of Agriculture and Life Sciences and Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul, Korea.
Yoye
Y
Yu
Agro-Biothechnology Research Institute, Jilin Academy of Agricultural Sciences, Changchun, China.
Yongmei
Y
Jin
Department of Plant Science, Research Institute of Agriculture and Life Sciences and Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul, Korea.
Mi-Ok
MO
Woo
Department of Applied Bio Science, Konkuk University, Seoul, Korea.
Joohyun
J
Lee
Department of Plant Science, Research Institute of Agriculture and Life Sciences and Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul, Korea.
Sunghan
S
Kim
Department of Plant Science, Research Institute of Agriculture and Life Sciences and Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul, Korea.
Hee-Jong
HJ
Koh
D000595:Amino Acid Sequence; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D002874:Chromosome Mapping; D003593:Cytoplasm / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D005799:Genes, Dominant; D017343:Genes, Plant / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D010940:Plant Proteins; D049452:Green Fluorescent Proteins