Structural characterization of polysaccharides expressed by Burkholderia oklahomensis E0147.
Burkholderia oklahomensis E0147 is a US isolated bacterium believed to express a similar O-antigen to type A structure of the highly pathogenic species, Burkholderia pseudomallei. Both species are genetically closely related. Lipopolysaccharide was collected from E0147 and structurally characterized to test this hypothesis. Glycosyl composition and linkage analyses in conjunction with 1D and 2D (1)H and (13)C NMR spectroscopy showed that the O-antigen was a repeating disaccharide with the following structure: [3)-β-D-Glcp-(1→3)-2OAc-α-L-6dTalp-(1→]n NMR spectroscopy also revealed the presence of a co-extracted exopolysaccharide previously described in B. pseudomallei, with the structure: [3)-2OAc-β-D-Galp-(1→4)-α-D-Galp-(1→3)-β-D-Galp-(1→5)-β-D-Kdop-(2→]n.
Department of Biological Sciences, Northern Arizona University, 1298 S. Knoles Dr. Building #56, Flagstaff, AZ 86011, USA.
Joshua K
JK
Stone
Complex Carbohydrate Research Center, The University of Georgia, Athens, GA 30602, USA.
Christian
C
Heiss
Complex Carbohydrate Research Center, The University of Georgia, Athens, GA 30602, USA.
Zhirui
Z
Wang
Complex Carbohydrate Research Center, The University of Georgia, Athens, GA 30602, USA.
Ian
I
Black
Department of Biological Sciences, Northern Arizona University, 1298 S. Knoles Dr. Building #56, Flagstaff, AZ 86011, USA.
Stephanie A
SA
Grasso
Department of Chemistry, Northern Arizona University, Flagstaff, AZ 86011, USA.
Andrew T
AT
Koppisch
Complex Carbohydrate Research Center, The University of Georgia, Athens, GA 30602, USA.
Parastoo
P
Azadi
Department of Biological Sciences, Northern Arizona University, 1298 S. Knoles Dr. Building #56, Flagstaff, AZ 86011, USA.
Paul
P
Keim
Department of Biological Sciences, Northern Arizona University, 1298 S. Knoles Dr. Building #56, Flagstaff, AZ 86011, USA. Electronic address: Apichai.Tuanyok@gmail.com.
Apichai
A
Tuanyok
D019117:Burkholderia / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*
D019081:O Antigens
Burkholderia oklahomensis; Exopolysaccharide; Lipopolysaccharide; NMR; O-antigen; Structure