Phylogenetic analysis of parasitic trematodes of the genus Euclinostomum found in Trichopsis and Betta fish.
Many species of fish in the world are infected with digenean trematodes belonging to the genera Clinostomum and Euclinostomum. In this study, metacercariae, identified as Euclinostomum sp. on the basis of morphological characteristics and molecular data, were taken from 3 osphronemid fish- Trichopsis vittata , Trichopsis schalleri, and Betta imbellis, in Thailand. Phylogenetic analysis based on a mitochondrial gene (cytochrome c oxidase subunit I) and 2 nuclear genes (18S rDNA and ITS-internal transcribed spacer) of these Euclinostomum parasites indicated a clear distinction from those belonging to the Clinostomum genus. These are the first records of partial mitochondrial and nuclear DNA sequences of an Euclinostomum sp.
National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani, 12120, Thailand;
S
S
Senapin
K
K
Phiwsaiya
P
P
Laosinchai
C
C
Kowasupat
P
P
Ruenwongsa
B
B
Panijpan
D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D021903:DNA, Ribosomal Spacer / Q000737:chemistry; D003576:Electron Transport Complex IV / Q000235:genetics; D005393:Fish Diseases / Q000469:parasitology*; D005399:Fishes; D008969:Molecular Sequence Data; D010802:Phylogeny; D018510:RNA, Helminth / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D016415:Sequence Alignment / Q000662:veterinary; D014200:Trematoda / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014201:Trematode Infections / Q000469:parasitology / Q000662:veterinary*
D018509:DNA, Helminth; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D018510:RNA, Helminth; D012337:RNA, Ribosomal, 18S; D003576:Electron Transport Complex IV