Analyzing the functions and structure of the human lipodystrophy protein seipin.
Disruption of the gene BSCL2, which encodes the protein seipin, causes severe generalized lipodystrophy in humans with a near complete absence of adipose tissue. Moreover, cell culture studies have demonstrated that seipin plays a critical cell-autonomous role in adipocyte differentiation. These observations reveal seipin as a critical regulator of human adipose tissue development; however, until recently very little has been known about the potential molecular functions of this intriguing protein. Despite significant recent interest in the function of seipin, our understanding of its molecular role(s) remains limited. The topology of seipin and lack of evidence for any enzymatic domains or activity indicate that it may act principally as a scaffold for other proteins or play a structural role in altering membrane curvature and/or budding. Work in this area has been hampered by several factors, including the lack of homology that might imply testable functions, the poor availability of antibodies to the endogenous protein and the observation that this hydrophobic ER membrane-resident protein is difficult to analyze by standard Western blotting techniques. Here we summarize some of the techniques we have applied to investigate the association of seipin with a recently identified binding partner, lipin 1. In addition, we describe the use of atomic force microscopy (AFM) to image oligomers of the seipin protein. We believe that AFM will offer a valuable tool to examine the association of candidate binding proteins with the seipin oligomer.
Institute of Metabolic Science, Addenbrooke's Hospital, University of Cambridge Metabolic Research Laboratories, Cambridge, United Kingdom.
M F Michelle
MF
Sim
Department of Pharmacology, University of Cambridge, Cambridge, United Kingdom.
Mesbah Uddin
MU
Talukder
Institute of Metabolic Science, Addenbrooke's Hospital, University of Cambridge Metabolic Research Laboratories, Cambridge, United Kingdom.
Rowena J
RJ
Dennis
Department of Pharmacology, University of Cambridge, Cambridge, United Kingdom.
J Michael
JM
Edwardson
Rowett Institute of Nutrition and Health, Institute of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom. Electronic address: j.rochford@abdn.ac.uk.
Justin J
JJ
Rochford
D017667:Adipocytes / Q000737:chemistry / Q000378:metabolism; D050156:Adipogenesis; D000273:Adipose Tissue / Q000254:growth & development* / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D004721:Endoplasmic Reticulum / Q000235:genetics / Q000473:pathology; D044388:GTP-Binding Protein gamma Subunits / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D050356:Lipid Metabolism / Q000235:genetics; D008060:Lipodystrophy / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018625:Microscopy, Atomic Force / Q000379:methods; D010711:Phosphatidate Phosphatase / Q000378:metabolism
C432679:BSCL2 protein, human; D044388:GTP-Binding Protein gamma Subunits; C485999:LPIN1 protein, human; D010711:Phosphatidate Phosphatase
Adipocyte; Adipogenesis; Atomic force microscopy; BSCL2; Bimolecular fluorescence; Lipin; Lipodystrophy; Seipin