Cadherin switching during the formation and differentiation of the Drosophila mesoderm - implications for epithelial-to-mesenchymal transitions.
Epithelial-to-mesenchymal transition (EMT) is typically accompanied by downregulation of epithelial (E-) cadherin, and is often additionally accompanied by upregulation of a mesenchymal or neuronal (N-) cadherin. Snail represses transcription of the E-cadherin gene both during normal development and during tumour spreading. The formation of the mesodermal germ layer in Drosophila, considered a paradigm of a developmental EMT, is associated with Snail-mediated repression of E-cadherin and the upregulation of N-cadherin. By using genetic manipulation to remove or overexpress the cadherins, we show here that the complementarity of cadherin expression is not necessary for the segregation or the dispersal of the mesodermal germ layer in Drosophila. However, we discover different effects of E- and N-cadherin on the differentiation of subsets of mesodermal derivatives, which depend on Wingless signalling from the ectoderm, indicating differing abilities of E- and N-cadherin to bind to and sequester the common junctional and signalling effector β-catenin. These results suggest that the downregulation of E-cadherin in the mesoderm might be required to facilitate optimal levels of Wingless signalling.
Institute of Genetics, University of Cologne, Zülpicher Strasse 47a, 50674 Cologne, Germany.
Gritt
G
Schäfer
Maithreyi
M
Narasimha
Elisabeth
E
Vogelsang
Maria
M
Leptin
D000818:Animals; D015820:Cadherins / Q000096:biosynthesis*; D002448:Cell Adhesion / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D004330:Drosophila / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000096:biosynthesis*; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology; D008648:Mesoderm / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
C570800:CadN protein, Drosophila; D015820:Cadherins; D029721:Drosophila Proteins; D014157:Transcription Factors; C501889:shg protein, Drosophila
Cell adhesion; Drosophila; Gastrulation; Wingless signalling; β-catenin