Effects of hydroxypropyl degree on physiochemical activities of chitosan from squid pens.
Chitosan was prepared by alkaline N-deacetylation of β-chitin and hydroxypropyl derivatives with different degrees of substitution (DS) were synthesized. It was characterized by Fourier transform infrared (FT-IR) and elemental analysis. The DS of hydroxypropyl chitosan (HPCS) calculated by an element analyzer were 0.42, 0.75, 1.20, 1.82 and 2.25. HPCS showed better foam capacity and stability than that of chitosan, and the effectiveness correlated well with the DS of HPCS. The highest bile acid-binding capacity of all five HPCS reached 56.02 mg/g, which was 4.0-fold higher than that of chitosan. The scavenging ability of HPCS against hydroxyl and ABTS radicals improved with increasing concentration. The correlation between the hydroxypropyl content (DS) of HPCS and scavenging ABTS radical ability was positive. The hydroxyl radicals scavenging activity of HPCS correlated well with its increasing concentration, and EC50 values were below 12.5 mg/mL. These results indicated that hydroxypropylation is a possible approach to obtain chitosan derivatives with desirable physiochemical properties.
Department of Food Science, Zhejiang University of Technology, Hangzhou 310014, PR China; Key Laboratory of Agricultural Products Chemical and Biological Processing Technology of Zhejiang Province, Hangzhou 310023, PR China.
Zhen-Hua
ZH
Cheng
Key Laboratory of Agricultural Products Chemical and Biological Processing Technology of Zhejiang Province, Hangzhou 310023, PR China. Electronic address: hjunlzr@163.com.
Jun
J
Huang
Department of Biological and Pharmaceutical Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, PR China.
Sheng
S
Hu
Key Laboratory of Agricultural Products Chemical and Biological Processing Technology of Zhejiang Province, Hangzhou 310023, PR China.
Cheng-Jun
CJ
Jiang
Key Laboratory of Agricultural Products Chemical and Biological Processing Technology of Zhejiang Province, Hangzhou 310023, PR China.
Qing
Q
Ge
Key Laboratory of Agricultural Products Chemical and Biological Processing Technology of Zhejiang Province, Hangzhou 310023, PR China.
Hong-Wu
HW
Lai
Key Laboratory of Agricultural Products Chemical and Biological Processing Technology of Zhejiang Province, Hangzhou 310023, PR China.
Jin-Yan
JY
Gong
Department of Food Science, Zhejiang University of Technology, Hangzhou 310014, PR China.
Pei-Long
PL
Sun
Key Laboratory of Agricultural Products Chemical and Biological Processing Technology of Zhejiang Province, Hangzhou 310023, PR China.
Jian-Wei
JW
Mao
Department of Biological and Pharmaceutical Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, PR China.
Le-He
LH
Mei
D000107:Acetylation; D000818:Animals; D052160:Benzothiazoles / Q000737:chemistry; D001647:Bile Acids and Salts / Q000378:metabolism; D055598:Chemical Phenomena; D048271:Chitosan / Q000737:chemistry* / Q000378:metabolism*; D049832:Decapodiformes / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000378:metabolism*; D017665:Hydroxyl Radical / Q000737:chemistry; D013329:Structure-Activity Relationship; D013451:Sulfonic Acids / Q000737:chemistry
D052160:Benzothiazoles; D001647:Bile Acids and Salts; D016166:Free Radical Scavengers; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D017665:Hydroxyl Radical; D048271:Chitosan
Degree of substitution; Hydroxypropyl chitosan; β-Chitin