[Quantitive variation of polysaccharides and alcohol-soluble extracts in F1 generation of Dendrobium officinale].
Using phenol-sulfuric acid method and hot-dip method of alcohol-soluble extracts, the contents of polysaccharides and alcohol-soluble extracts in 11 F1 generations of Dendrobium officinale were determined. The results showed that the polysaccharides contents in samples collected in May and February were 32.89%-43.07% and 25.77%-35.25%, respectively, while the extracts contents were 2.81%-4.85% and 7.90%-17.40%, respectively. They were significantly different among families. The content of polysaccharides in offspring could be significantly improved by hybridization between parents with low and high polysaccharides contents, and the hybrid vigor was obvious. Cross breeding was an effective way for breeding new varieties with higher polysaccharides contents. Harvest time would significantly affect the contents of polysaccharides and alcohol-soluble extracts. The contents of polysaccharides in families collected in May were higher than those of polysaccharides in families collected in February, but the extracts content had the opposite variation. The extents of quantitative variation of polysaccharides and alcohol-soluble extracts were different among families, and each family had its own rules. It would be significant in giving full play to their role as the excellent varieties and increasing effectiveness by studying on the quantitative accumulation regularity of polysaccharides and alcohol-soluble extracts in superior families (varieties) of D. officinale to determine the best harvesting time.
Nurturing Station for State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Key Laboratory of Varieties Created and Ecological Cultivation of Dendrobium officinale for National Chinese Traditional Medicine Administrative Bureau, Zhejiang Provincial Strategic Alliance for Technical Innovation in Industry of Dendrobium officinale, Lin'an 311300, China.
Xiao-Ling
XL
Zhang
Nurturing Station for State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Key Laboratory of Varieties Created and Ecological Cultivation of Dendrobium officinale for National Chinese Traditional Medicine Administrative Bureau, Zhejiang Provincial Strategic Alliance for Technical Innovation in Industry of Dendrobium officinale, Lin'an 311300, China.
Jing-Jing
JJ
Liu
Nurturing Station for State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Key Laboratory of Varieties Created and Ecological Cultivation of Dendrobium officinale for National Chinese Traditional Medicine Administrative Bureau, Zhejiang Provincial Strategic Alliance for Technical Innovation in Industry of Dendrobium officinale, Lin'an 311300, China.
Ling-Shang
LS
Wu
Nurturing Station for State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Key Laboratory of Varieties Created and Ecological Cultivation of Dendrobium officinale for National Chinese Traditional Medicine Administrative Bureau, Zhejiang Provincial Strategic Alliance for Technical Innovation in Industry of Dendrobium officinale, Lin'an 311300, China.
Jin-Ping
JP
Si
Nurturing Station for State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Key Laboratory of Varieties Created and Ecological Cultivation of Dendrobium officinale for National Chinese Traditional Medicine Administrative Bureau, Zhejiang Provincial Strategic Alliance for Technical Innovation in Industry of Dendrobium officinale, Lin'an 311300, China.
Ying-Ying
YY
Guo
Fenghua Jiuwei Shengcaotang Professinal Cooperatives of Dendrobium officinale, Fenghua 315500, China.
Jie
J
Yu
Zhejiang Jiacheng Biological Engineering Co., Ltd., Yiwu 322002, China.
Lin-Hua
LH
Wang
D001947:Breeding; D002681:China; D031667:Dendrobium / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification
D010936:Plant Extracts; D011134:Polysaccharides