Antimicrobial function of SHβAP, a novel hemoglobin β chain-related antimicrobial peptide, isolated from the liver of skipjack tuna, Katsuwonus pelamis.
A 2.3 kDa of antimicrobial peptide was purified from an acidified liver extract of skipjack tuna, Katsuwonus pelamis, by preparative acid-urea-polyacrylamide gel electrophoresis and C18 reversed-phase HPLC. A comparison of the amino acid sequence of the purified peptide with those of other known polypeptides revealed high homology with the C-terminus of hemoglobin β-chain; thus, this peptide was designated as the Skipjack Hemoglobin β chain-related Antimicrobial Peptide (SHβAP). SHβAP showed potent antimicrobial activity against Gram-positive bacteria, such as Bacillus subtilis, Staphylococcus aureus, and Streptococcus iniae (minimal effective concentrations [MECs], 6.5-57.0 μg/mL), Gram-negative bacteria, such as Escherichia coli D31, Pseudomonas aeruginosa, Salmonella enterica, Shigella sonnei, and two Vibrio parahaemolyticus species (MECs, 2.0-19.0 μg/mL), and against Candida albicans (MEC; 12.0 μg/mL) without significant hemolytic activity. Antimicrobial activity of this peptide was heatstable and pH resistant but is sensitive to proteases and salt. SHβAP did not show membrane permeabilization and killing ability. The secondary structural prediction and the homology modeling expected that this peptide formed an amphipathic α-helical structure. This is the first report the purification of a novel antimicrobial peptide related to the C-terminus of hemoglobin β-chain from marine fish.
Department of Food Science and Biotechnology, Kunsan National University, Kunsan 573-701, Republic of Korea.
Jung-Kil
JK
Seo
Department of Biotechnology, Pukyong National University, Busan 608-737, Republic of Korea.
Min Jeong
MJ
Lee
Department of Biotechnology, Pukyong National University, Busan 608-737, Republic of Korea.
Hyun-Gyo
HG
Jung
Department of Biotechnology, Pukyong National University, Busan 608-737, Republic of Korea.
Hye-Jin
HJ
Go
Korea Environmental Industry and Technology Institute, Seoul 122-706, Republic of Korea.
Young Ja
YJ
Kim
Department of Biotechnology, Pukyong National University, Busan 608-737, Republic of Korea. Electronic address: ngpark@pknu.ac.kr.
Nam Gyu
NG
Park
D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D019295:Computational Biology; D004591:Electrophoresis, Polyacrylamide Gel; D006461:Hemolysis / Q000187:drug effects; D008081:Liposomes / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014413:Tuna / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D055544:beta-Globins / Q000378:metabolism*
D023181:Antimicrobial Cationic Peptides; D008081:Liposomes; D055544:beta-Globins
Antimicrobial peptide; Hemoglobin; Innate immunity; Skipjack tuna liver