Caffeoylquinic acids in leaves of selected Apocynaceae species: Their isolation and content.
BACKGROUND
Three compounds isolated from the methanol (MeOH) leaf extract of Vallaris glabra (Apocynaceae) were those of caffeoylquinic acids (CQAs). This prompted a quantitative analysis of their contents in leaves of V. glabra in comparison with those of five other Apocynaceae species (Alstonia angustiloba, Dyera costulata, Kopsia fruticosa, Nerium oleander, and Plumeria obtusa), including flowers of Lonicera japonica (Japanese honeysuckle), the commercial source of chlorogenic acid (CGA).


METHODS
Compound were isolated by column chromatography, and identified by NMR and MS analyses. CQA content of leaf extracts was determined using reversed-phase HPLC.


RESULTS
From the MeOH leaf extract of V. glabra, 3-CQA, 4-CQA, and 5-CQA or CGA were isolated. Content of 5-CQA of V. glabra was two times higher than flowers of L. japonica, while 3-CQA and 4-CQA content was 16 times higher.


CONCLUSIONS
With much higher CQA content than the commercial source, leaves of V. glabra can serve as a promising alternative source.
School of Science, Monash University Sunway Campus, Bandar Sunway, 46150 Petaling Jaya, Selangor, Malaysia.
Siu Kuin
SK
Wong
School of Science, Monash University Sunway Campus, Bandar Sunway, 46150 Petaling Jaya, Selangor, Malaysia.
Yau Yan
YY
Lim
Natural Products Division, Forest Research Institute Malaysia, Kepong, 52109 Selangor, Malaysia.
Sui Kiong
SK
Ling
Faculty of Applied Sciences, UCSI University, 56000 Cheras, Kuala Lumpur, Malaysia.
Eric Wei Chiang
EW
Chan
Apocynaceae; Vallaris glabra; caffeoylquinic acids; chlorogenic acid