RANKL-based osteoclastogenic assays from murine bone marrow cells.
Osteoclasts are the only cell type in the body to resorb bone. Osteoclasts play a critical role in physiologic and pathologic bone remodeling. Many genetic mouse models affect the skeleton by regulating osteoclast function directly or indirectly. This protocol describes a procedure for generating osteoclasts from mouse bone marrow cells using macrophage colony stimulating factor and receptor activator of NF-κB ligand.
University of Rochester Medical Center, Rochester, NY, USA.
Lianping
L
Xing
University of Rochester Medical Center, Rochester, NY, USA.
Brendan F
BF
Boyce
D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism*; D001862:Bone Resorption / Q000378:metabolism*; D006651:Histocytochemistry; D051379:Mice; D010010:Osteoclasts / Q000378:metabolism*; D061251:Primary Cell Culture; D053245:RANK Ligand / Q000378:metabolism*
D053245:RANK Ligand