Enzymatic and non-enzymatic antioxidant potentials of Chlorella vulgaris grown in effluent of a confectionery industry.
Enzymatic and non-enzymatic antioxidant potentials of Chlorella vulgaris have gained considerable importance in recent decades. C. vulgaris strain highly tolerant to extreme pH variations was isolated and mass-cultivated in the wastewater from a confectionery industry. C.vulgaris showed better growth in wastewater than in improvised CFTRI medium. The microalgal biomass was then screened for the following antioxidants: peroxidase, superoxide dismutase, polyphenol oxidase, glutathione peroxidase, chlorophyll a, ascorbic acid, α-tocopherol and reduced glutathione. The total polyphenol content of the strain was also studied. The strain showed a high degree of enzymatic antioxidant activity (0.195 × 10(-5) ± 0.0072 units/cell peroxidase, 0.04125 × 10(-5) ± 0.001 units/cell superoxide dismutase, 0.2625 × 10(-5) ± 0.003 units/cell polyphenol oxidase and 0.025 × 10(-5) ± 0.003 glutathione peroxidase). The microalgal biomass also showed, per milligram weight, 0.2182 ± 0.005 μg of ascorbic acid, 0.00264 ± 0.001 μg of α-tocopherol and 0.07916 ± 0.004 μg of reduced glutathione. These results represent the possibility of using C. vulgaris grown in confectionery industry wastewater as a source of nutritious supplement, which is highly promising in terms of both economic and nutritional point of view.
Department of Plant Biology and Plant Biotechnology, Ramakrishna Mission Vivekananda College, Chennai, 600 004 Tamil Nadu India.
R Ranjith
RR
Kumar
Department of Plant Biology and Plant Biotechnology, Ramakrishna Mission Vivekananda College, Chennai, 600 004 Tamil Nadu India.
P Hanumantha
PH
Rao
Department of Plant Biology and Plant Biotechnology, Ramakrishna Mission Vivekananda College, Chennai, 600 004 Tamil Nadu India.
V V
VV
Subramanian
Department of Plant Biology and Plant Biotechnology, Ramakrishna Mission Vivekananda College, Chennai, 600 004 Tamil Nadu India.
V
V
Sivasubramanian
Chlorella vulgaris; Confectionery industry effluent; Enzymatic antioxidants; Non-enzymatic antioxidants