CORRELATION BETWEEN MICRO-CT SECTIONS AND HISTOLOGICAL SECTIONS OF MOUSE SKULL DEFECTS IMPLANTED WITH ENGINEERED CARTILAGE.
One advantage of using cartilage to replace/repair bone is that the implant disappears as bone is formed by endochondral ossification. Previously, we showed that cartilage spheroids, grown in a rotating bioreactor (Synthecon, Inc.) and implanted into a 2 mm skull defect, contributed to healing of the defect. Skulls with or without implants were subjected to microCT scans. Mineralized regions from microCT sections correlated with regions of bone in histological sections of the defect region of demineralized skulls. Recently, sections from microCT scans of live mice were compared to histological sections from the same mice. The area of the defect staining for bone in histological sections of demineralized skulls was the same region shown as mineralized in microCT sections. Defects without implants were not healed. This study demonstrates that microCT scans are an important corollary to histological studies evaluating the use of implants in healing of bony defects.
Department of Orthodontics, Dental Branch, UTHSC, Houston, TX.
P J
PJ
Duke
Department of Orthodontics, Dental Branch, UTHSC, Houston, TX.
L
L
Doan
Department of Orthodontics, Dental Branch, UTHSC, Houston, TX.
H
H
Luong
Department of Orthodontics, Dental Branch, UTHSC, Houston, TX.
C
C
Kelley
Our Lady of the Lake College, Baton Rouge, LA.
W
W
Leboeuf
University of Houston, Houston, TX.
Q
Q
Diep
Dept of Imaging Physics, MD Anderson Cancer Center, Houston, TX 77225, U.S.A.
E
E
Johnson
Dept of Imaging Physics, MD Anderson Cancer Center, Houston, TX 77225, U.S.A.
D D
DD
Cody