Arsenic-Induced Hepatic Toxicity and Its Attenuation by Fruit Extract of Emblica officinalis (Amla) in Mice.
Arsenic a metalloid and environmental contaminated has been found to be associated with public health problems in the affected areas. It is naturally occurred in groundwater and its accumulation in plant and animals leads to toxicity in several tissues most notably hepatic organ. Arsenic exposures (3 mg/kg body weight/day for 30 days) in mice exhibited increased arsenic and Zn levels in hepatocytes associated with enhanced oxidative stress in hepatocytes while there were no significantly changes were observed in Cu level. An increase in the lipid peroxidation and decrease in the levels of reduced glutathione and activity of superoxide dismutase, catalase, and glutathione peroxidase were observed in arsenic treated mice as compared to controls. Arsenic exposure in mice also caused a significant change in serum biomarkers in the SGOT, SGPT and creatinine as compared to the controls. There were no significant changes in the serum levels of total protein in these mice. Co-administration of arsenic and fruit extract of amla (500 mg/kg body weight/day for 30 days) caused a significant reduction of arsenic transference associated with significantly decreases hepatic arsenic levels and balanced the antioxidant enzyme and levels of serum hepatic enzymes like SGOT and SGPT. The results of the present study clearly demonstrate the antioxidant property of amla that could be responsible for its protective efficacy in arsenic induced hepatic toxicity.
Department of Pharmacology, King George's Medical University, Lucknow, 226 003 India.
Manish K
MK
Singh
Department of Biochemistry, All India Institute of Medical Sciences, Jodhpur, 342005 India.
Shailendra
S
Dwivedi
Department of Pharmacology, King George's Medical University, Lucknow, 226 003 India.
Suraj S
SS
Yadav
Department of Biochemistry, All India Institute of Medical Sciences, Jodhpur, 342005 India.
Praveen
P
Sharma
Department of Pharmacology, King George's Medical University, Lucknow, 226 003 India.
Sanjay
S
Khattri
Arsenic; Emblicaofficinalis; Liver dysfunction; Metal estimation; Mice; Oxidative stress