Novel electrochemical sensing platform based on magnetic field-induced self-assembly of Fe3O4@Polyaniline nanoparticles for clinical detection of creatinine.
A novel electrochemical sensing platform based on magnetic field-induced self-assembly of Fe3O4@Polyaniline nanoparticles (Fe3O4@PANI NPs) has been for the first time fabricated for the sensitive detection of creatinine in biological fluids. The template molecule, creatinine, was self-assembled on the surface of Fe3O4@PANI NPs together with the functional monomer aniline by the formation of N-H hydrogen bonds. After pre-assembled, through the magnetic-induction of the magnetic glassy carbon electrode (MGCE), the ordered structure of molecularly imprinted polymers (MIPs) were established by the electropolymerization and assembled on the surface of MGCE with the help of magnetic fields by a simple one-step approach. The structural controllability of the MIPs film established by magnetic field-induced self-assembly was further studied. The stable and hydrophilic Fe3O4@PANI can not only provide available functionalized sites with which the template molecule creatinine can form hydrogen bond by the abundant amino groups in PANI matrix, but also afford a promoting pathway for electron transfer. The as-prepared molecularly imprinted electrochemical sensor (MIES) shows good stability and reproducibility for the determination of creatinine with the detection limit reached 0.35 nmol L(-1) (S/N=3). In addition, the highly sensitive and selective MIES has been successfully used for the clinical determination of creatinine in human plasma and urine samples. The average recoveries were 90.8-104.9% with RSD lower than 2.7%.
School of Pharmacy, Nanjing Medical University, Nanjing 210029, PR China; The First People's Hospital of Kunshan, Kunshan 215300, PR China.
Tingting
T
Wen
School of Pharmacy, Nanjing Medical University, Nanjing 210029, PR China.
Wanying
W
Zhu
School of Pharmacy, Nanjing Medical University, Nanjing 210029, PR China.
Cheng
C
Xue
School of Pharmacy, Nanjing Medical University, Nanjing 210029, PR China.
Jinhua
J
Wu
School of Pharmacy, Nanjing Medical University, Nanjing 210029, PR China.
Qing
Q
Han
School of Pharmacy, Nanjing Medical University, Nanjing 210029, PR China.
Xi
X
Wang
School of Pharmacy, Nanjing Medical University, Nanjing 210029, PR China. Electronic address: xueminzhou001_001@hotmail.com.
Xuemin
X
Zhou
School of Pharmacy, Nanjing Medical University, Nanjing 210029, PR China. Electronic address: huijun_jiang2006@hotmail.com.
Huijun
H
Jiang
D000814:Aniline Compounds / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D003404:Creatinine / Q000032:analysis / Q000097:blood*; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D054802:Molecular Imprinting; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results
D000814:Aniline Compounds; C416807:polyaniline; D003404:Creatinine; C023650:aniline; D052203:Ferrosoferric Oxide
Creatinine; Electrochemical sensor; Magnetic field-induced; Molecularly imprinted polymers; Self-assembly