GABAergic and glycinergic inputs modulate rhythmogenic mechanisms in the lamprey respiratory network.
We have previously shown that GABA and glycine modulate respiratory activity in the in vitro brainstem preparations of the lamprey and that blockade of GABAA and glycine receptors restores the respiratory rhythm during apnoea caused by blockade of ionotropic glutamate receptors. However, the neural substrates involved in these effects are unknown. To address this issue, the role of GABAA, GABAB and glycine receptors within the paratrigeminal respiratory group (pTRG), the proposed respiratory central pattern generator, and the vagal motoneuron region was investigated both during apnoea induced by blockade of glutamatergic transmission and under basal conditions through microinjections of specific antagonists. The removal of GABAergic, but not glycinergic transmission within the pTRG, causes the resumption of rhythmic respiratory activity during apnoea, and reveals the presence of a modulatory control of the pTRG under basal conditions. A blockade of GABAA and glycine receptors within the vagal region strongly increases the respiratory frequency through disinhibition of neurons projecting to the pTRG from the vagal region. These neurons were retrogradely labelled (neurobiotin) from the pTRG. Intense GABA immunoreactivity is observed both within the pTRG and the vagal area, which corroborates present findings. The results confirm the pTRG as a primary site of respiratory rhythm generation, and suggest that inhibition modulates the activity of rhythm-generating neurons, without any direct role in burst formation and termination mechanisms.
Dipartimento di Medicina Sperimentale e Clinica, Sezione Scienze Fisiologiche, Università degli Studi di Firenze, Viale G.B. Morgagni 63, 50134 Firenze, Italy.  fulvia.bongianni@unifi.it.
Elenia
E
Cinelli
Donatella
D
Mutolo
Brita
B
Robertson
Sten
S
Grillner
Massimo
M
Contini
Tito
T
Pantaleo
Fulvia
F
Bongianni
D000200:Action Potentials; D000818:Animals; D063226:Central Pattern Generators / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D018756:GABA Antagonists / Q000494:pharmacology; D059330:GABAergic Neurons / Q000378:metabolism / Q000502:physiology; D007798:Lampreys; D009046:Motor Neurons / Q000378:metabolism / Q000502:physiology; D018079:Receptors, GABA / Q000235:genetics / Q000378:metabolism*; D018009:Receptors, Glycine / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D012119:Respiration; D014630:Vagus Nerve / Q000166:cytology / Q000187:drug effects / Q000502:physiology
D018756:GABA Antagonists; D018079:Receptors, GABA; D018009:Receptors, Glycine