Identification of potential vaccine candidates against Streptococcus pneumoniae by reverse vaccinology approach.
In the past few decades, genome-based approaches have contributed significantly to vaccine development. Our aim was to identify the most conserved and immunogenic antigens of Streptococcus pneumoniae, which can be potential vaccine candidates in the future. BLASTn was done to identify the most conserved antigens. PSORTb 3.0.2 was run to predict the subcellular localization of the proteins. B cell epitope prediction was done for the immunogenicity testing. Finally, BLASTp was done for verifying the extent of similarity to human proteome to exclude the possibility of autoimmunity. Proteins failing to comply with the set parameters were filtered at each step. Based on the above criteria, out of the initial 22 pneumococcal proteins selected for screening, pavB and pullulanase were the most promising candidate proteins.
Department of Biotechnology & Bioengineering, IST, Gauhati University, Jalukbari, Guwahati, Assam, India, 781014.
Sandipan
S
Talukdar
Shubhranshu
S
Zutshi
K S
KS
Prashanth
Kandarpa K
KK
Saikia
Parveen
P
Kumar
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology / Q000382:microbiology; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D019295:Computational Biology; D017124:Conserved Sequence; D018985:Epitopes, B-Lymphocyte / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D016680:Genome, Bacterial / Q000276:immunology*; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D011008:Pneumococcal Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control; D022242:Pneumococcal Vaccines / Q000235:genetics / Q000276:immunology; D020543:Proteome / Q000235:genetics / Q000276:immunology; D013296:Streptococcus pneumoniae / Q000235:genetics* / Q000276:immunology; D037521:Virulence Factors / Q000737:chemistry* / Q000235:genetics / Q000276:immunology
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D018985:Epitopes, B-Lymphocyte; D022242:Pneumococcal Vaccines; D020543:Proteome; D037521:Virulence Factors; D006026:Glycoside Hydrolases; C100181:pullulanase