Protein a detection based on quantum dots-antibody bioprobe using fluorescence coupled capillary electrophoresis.
In this report, fluorescence detection coupled capillary electrophoresis (CE-FL) was used to detect Protein A. Antibody was first labeled with Cy5 and then mixed with quantum dots (QDs) to form QDs-antibody bioprobe. Further, we observed fluorescence resonance energy transfer (FRET) from QDs donor to Cy5 acceptor. The bioprobe was formed and brought QDs and Cy5 close enough to allow FRET to occur. After adding protein A, the FRET system was broken and caused the FRET signal to decrease. Thus, a new method for the determination of protein A was proposed based on the FRET signal changes. This study provides a new trail of thought for the detection of protein.
State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China. linqiupjj@gmail.com.
Lin
L
Qiu
Shandong Academy of Medical Sciences, Jinan 250062, China. byh168@163.com.
Yanhua
Y
Bi
School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou 213164, China. clwang@cczu.edu.cn.
Cheli
C
Wang
School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou 213164, China. jingyan.leee@gmail.com.
Jingyan
J
Li
School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou 213164, China. 351533126@163.com.
Peilin
P
Guo
School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou 213164, China. jingyan.leee@gmail.com.
Jinchen
J
Li
State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China. heweij69@nju.edu.cn.
Weijiang
W
He
School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou 213164, China. minuswan@gmail.com.
Jianhao
J
Wang
School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou 213164, China. pengju.jiang@gmail.com.
Pengju
P
Jiang
D000906:Antibodies / Q000737:chemistry* / Q000378:metabolism; D015374:Biosensing Techniques; D002232:Carbocyanines / Q000737:chemistry; D019075:Electrophoresis, Capillary; D031541:Fluorescence Resonance Energy Transfer; D007074:Immunoglobulin G / Q000737:chemistry / Q000378:metabolism; D045663:Quantum Dots / Q000737:chemistry*; D013205:Staphylococcal Protein A / Q000737:chemistry* / Q000378:metabolism
D000906:Antibodies; D002232:Carbocyanines; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A; C085321:cyanine dye 5