Evaluation of pleural effusion sCD26 and DPP-IV as diagnostic biomarkers in lung disease.
In this study, we measured ADA and DPP-IV enzymatic activity and sCD26 concentration in 150 pleural effusion (PE) samples and tested for correlations between these and other cellular and biochemical measures. We found that DPP-IV in particular might improve the specificity (but not the sensitivity) of the ADA test for diagnosis of pulmonary tuberculosis, since half of the false ADA positive results in non-tuberculous PE were also DPP-IV positive. A percentage of patients with malignant PE were sCD26 or DPP-IV positive; however, some patients with benign PE also tested positive. As a pattern associated with DPP-IV (but not the CD26 protein) was observed in PE, we searched for a finding that might increase the value of these biomarkers for diagnosis of malignancy. The observed pattern was related to the presence of leukocytes, as indicated by correlations with the cell count, and to a band of 180 kDa, detected by immunoblotting.
Department of Biochemistry, Genetics and Immunology, Faculty of Biology, University of Vigo, 36310 Vigo (Spain).
Nuria
N
Sánchez-Otero
Department of Biochemistry, Genetics and Immunology, Faculty of Biology, University of Vigo, 36310 Vigo (Spain).
Francisco Javier
FJ
Rodríguez-Berrocal
Department of Biochemistry, Genetics and Immunology, Faculty of Biology, University of Vigo, 36310 Vigo (Spain).
María Páez
MP
de la Cadena
Unit of Interventional Bronchopleural Pathology, Pneumology Department of Hospital University Complex of Vigo (CHUVI), 36214 Vigo (Spain).
María Isabel
MI
Botana-Rial
Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Santiago de Compostela, 15782 Santiago de Compostela (Spain).
Oscar J
OJ
Cordero
D000243:Adenosine Deaminase / Q000096:biosynthesis*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers; D018819:Dipeptidyl Peptidase 4 / Q000096:biosynthesis*; D005122:Exudates and Transudates; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D010996:Pleural Effusion / Q000378:metabolism*; D018417:Th1 Cells / Q000276:immunology; D014396:Tuberculosis, Pleural / Q000175:diagnosis* / Q000276:immunology; D055815:Young Adult
D015415:Biomarkers; D007371:Interferon-gamma; C042807:DPP4 protein, human; D018819:Dipeptidyl Peptidase 4; D000243:Adenosine Deaminase