Antioxidant and immunoregulatory activity of alkali-extractable polysaccharides from North American ginseng.
The alkali-extractable polysaccharide (AEP) was isolated from the root of North American ginseng. Two fractions, AEP-1 and AEP-2, were further purified by gel filtration column chromatography. Gas chromatography analysis identified that AEP-1 was composed of Glc, Gal and GalA. And AEP-2 mainly contained Ara, Man, Gal, Glc and GalA. Antioxidant assays indicated that AEP and AEP-2 exhibited significant antioxidant activities in a dose-dependent manner. AEP-2 also exhibited macrophage-activating activity by increasing NO, TNF-α and IL-6 production. The results suggest that AEP-2 could be used as potential antioxidants and immunomodulators.
Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; School of Food & Bioengineering, Qilu University of Technology, Jinan 250353, China.
Xiaona
X
Yu
Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Research Center for Environmental Science and Engineering, Shanxi University, Taiyuan 030006, China.
Xiushi
X
Yang
School of Food & Bioengineering, Qilu University of Technology, Jinan 250353, China.
Bo
B
Cui
Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; School of Life Sciences, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China.
Lijun
L
Wang
Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address: renguixing@caas.cn.
Guixing
G
Ren
D000468:Alkalies / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D007155:Immunologic Factors / Q000302:isolation & purification / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D005894:Panax / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*
D000468:Alkalies; D000975:Antioxidants; D016207:Cytokines; D007155:Immunologic Factors; D011134:Polysaccharides; D009569:Nitric Oxide
Alkali-extractable polysaccharide; Bioactivity; North American ginseng