Characterization of cattle fecal Streptomyces strains converting cellulose and hemicelluloses into reducing sugars.
To characterize Streptomyces isolated from cattle feces for converting lignocellulose into reducing sugars, five Streptomyces strains were screened. All the strains could convert lignocellulose into reducing sugars. The strain A16 accumulate 3.3-folds more reducing sugars on cottonseed shells treated with ethanol than without the treatment (P < 0.05). The five strains did not accumulate more reducing sugars on rice straws and wheat brans than those on cottonseed shells. Compared with A10 alone, the microbial combination of F1 + A10 accumulated 19, 61, and 25 % less reducing sugars on cottonseed shell, rice straw, and wheat bran than those by A10 solely, respectively (P < 0.05). Further studies indicated that the activities of avicelase and xylanase were not correlated with the reducing sugar amount accumulated by the test strains. Strain A7 could produce more cellular lipids with xylose and glucose as the sole carbon sources. This study shows the potential for Streptomyces strains from herbivore feces to convert lignocelluloses into lipids and reducing sugars for fuel production.
School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, People's Republic of China.
Yupei
Y
Liu
Zujun
Z
Deng
Hongming
H
Tan
Qingli
Q
Deng
Lixiang
L
Cao
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002482:Cellulose / Q000378:metabolism*; D005243:Feces / Q000382:microbiology*; D005947:Glucose / Q000378:metabolism; D008031:Lignin / Q000378:metabolism; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000378:metabolism*; D013302:Streptomyces / Q000145:classification* / Q000254:growth & development / Q000502:physiology*
D011134:Polysaccharides; C036909:lignocellulose; C007916:hemicellulose; D002482:Cellulose; D008031:Lignin; D005947:Glucose