Aseptic meningitis outbreak caused by echovirus 30 in two regions in Bulgaria, May-August 2012.
An aseptic meningitis outbreak emerged in two regions in Bulgaria in 2012 and echovirus 30 (E30) was established as the aetiological agent by cell culture isolation, serological test, and molecular-based techniques. A total of 157 patients with aseptic meningitis were investigated, of which 117 were confirmed as having E30-associated disease. Molecular analysis of 12 E30 isolates revealed 99-100% nucleotide and amino-acid identity between them and a close correlation with a Greek strain involved in an E30 outbreak in 2012. Children aged 5-14 years were mainly affected, which could reflect the absence of E30 epidemics in Bulgaria for a period of 11 years. The first case with E30 isolation (a 2-year-old patient from Plovdiv) was notified at the end of April 2012. This was most likely the index case, from which the spread of the virus started, causing sporadic cases first, which later led to an aseptic meningitis outbreak facilitated by person-to-person viral transmission.
Department of Virology, National Center for Infectious and Parasitic Diseases, Sofia,Bulgaria.
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Z
Mladenova
National Centre for Immunobiologicals Research and Evaluation, Istituto Superiore di Sanità, Rome,Italy.
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G
Buttinelli
Department of Virology, National Center for Infectious and Parasitic Diseases, Sofia,Bulgaria.
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A
Dikova
Department of Virology, National Center for Infectious and Parasitic Diseases, Sofia,Bulgaria.
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A
Stoyanova
Department of Infectious Diseases Surveillance, Regional Health Inspectorate, Plovdiv,Bulgaria.
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M
Troyancheva
Infectious Ward,University Multi-Profiled Hospital 'St. George',Plovdiv,Bulgaria.
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R
Komitova
Infectious Ward,University Multi-Profiled Hospital 'St. George',Plovdiv,Bulgaria.
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M
Stoycheva
Multi-Profiled Hospital for Active Treatment 'Prof. St. Kirkovich',Stara Zagora,Bulgaria.
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L
Pekova
Department of Epidemiology and CD surveillance, Epidemiology Unit, National Centre for Infectious and Parasitic Diseases, Sofia,Bulgaria.
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K
Parmakova
National Centre for Immunobiologicals Research and Evaluation, Istituto Superiore di Sanità, Rome,Italy.
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L
Fiore
D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D002031:Bulgaria / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D004196:Disease Outbreaks; D004457:Echovirus Infections / Q000453:epidemiology* / Q000821:virology; D029822:Enterovirus B, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D008582:Meningitis, Aseptic / Q000453:epidemiology*; D008875:Middle Aged; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D055815:Young Adult
D012367:RNA, Viral