Short-term effects of 7-ketocholesterol on human adipose tissue mesenchymal stem cells in vitro.
Oxysterols comprise a very heterogeneous group derived from cholesterol through enzymatic and non-enzymatic oxidation. Among them, 7-ketocholesterol (7-KC) is one of the most important. It has potent effects in cell death processes, including cytoxicity and apoptosis induction. Mesenchymal stem cells (MSCs) are multipotent cells characterized by self-renewal and cellular differentiation capabilities. Very little is known about the effects of oxysterols in MSCs. Here, we describe the short-term cytotoxic effect of 7-ketocholesterol on MSCs derived from human adipose tissue. MSCs were isolated from adipose tissue obtained from two young, healthy women. After 24 h incubation with 7-KC, mitochondrial hyperpolarization was observed, followed by a slight increase in the level of apoptosis and changes in actin organization. Finally, the IC50 of 7-KC was higher in these cells than has been observed or described in other normal or cancer cell lines.
Laboratory of Genetics and Molecular Hematology (LIM31), University of São Paulo School of Medicine, Av. Dr. Enéas de Carvalho Aguiar,155, 1st Floor, Room 43, 05403-000 São Paulo, SP, Brazil.
Débora
D
Levy
Laboratory of Genetics and Molecular Hematology (LIM31), University of São Paulo School of Medicine, Av. Dr. Enéas de Carvalho Aguiar,155, 1st Floor, Room 43, 05403-000 São Paulo, SP, Brazil.
Jorge Luis Maria
JL
Ruiz
Laboratory of Genetics and Molecular Hematology (LIM31), University of São Paulo School of Medicine, Av. Dr. Enéas de Carvalho Aguiar,155, 1st Floor, Room 43, 05403-000 São Paulo, SP, Brazil.
Andrea Turbuck
AT
Celestino
Laboratory of Genetics and Molecular Hematology (LIM31), University of São Paulo School of Medicine, Av. Dr. Enéas de Carvalho Aguiar,155, 1st Floor, Room 43, 05403-000 São Paulo, SP, Brazil.
Suelen Feitoza
SF
Silva
Departament of Immunology, Laboratory of Tumor Immunology, Institute of Biomedical Science, University of Sao Paulo, Av. Prof. Lineu Prestes, 1730-Room 254, 05508-900 São Paulo, SP, Brazil.
Adilson Kleber
AK
Ferreira
Cell Culture and Wound Healing Research Laboratory, Division of Plastic Surgery, University of São Paulo, Av. Dr. Arnaldo, 455, 1st Floor, 05403-000 São Paulo, SP, Brazil.
Cesar
C
Isaac
Laboratory of Genetics and Molecular Hematology (LIM31), University of São Paulo School of Medicine, Av. Dr. Enéas de Carvalho Aguiar,155, 1st Floor, Room 43, 05403-000 São Paulo, SP, Brazil. Electronic address: spbydlow@usp.br.
Sérgio Paulo
SP
Bydlowski
D000199:Actins / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects; D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007653:Ketocholesterols / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000187:drug effects*; D055815:Young Adult
D000199:Actins; D007653:Ketocholesterols; C003001:7-ketocholesterol
7-Ketocholesterol; Adipose tissue; Apoptosis; Mesenchymal stem cell; Mitochondrial hyperpolarization