Senescing human bone-marrow-derived clonal mesenchymal stem cells have altered lysophospholipid composition and functionality.
Mesenchymal stem cells (MSCs) have been used in a wide range of research and clinical studies because MSCs do not have any ethical issues and have the advantage of low carcinogenicity due to their limited proliferation. However, because only a small number of MSCs can be obtained from the bone marrow, ex vivo amplification is inevitably required. For that reason, this study was conducted to acquire the metabolic information to examine and control the changes in the activities and differentiation potency of MSCs during the ex vivo culture process. Endogenous metabolites of human bone-marrow-derived clonal MSCs (hcMSCs) during cellular senescence were profiled by ultraperformance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/QTOFMS). To select significant metabolites, we used the linear mixed effects model having fixed effects for batch and time (passage) and random effects for metabolites, determining the mean using a t test and the standard deviation using an F test. We used structural analysis with representative standards and spectrum patterns with different collision energies to distinctly identify eight metabolites with altered expression during senescence as types of lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine (LPE), such as LPC 16:0 and LPE 22:4. The present study revealed changes in endogenous metabolites and mechanisms due to senescence.
College of Pharmacy, Seoul National University , 599 Gwanangno, Gwanak-gu, Seoul 151742, Korea.
Seul Ji
SJ
Lee
TacGhee
T
Yi
Soo Hyun
SH
Ahn
Dong Kyu
DK
Lim
Ji Yeon
JY
Hong
Yun Kyoung
YK
Cho
Johan
J
Lim
Sun U
SU
Song
Sung Won
SW
Kwon
D001854:Bone Marrow Cells / Q000737:chemistry / Q000166:cytology; D002454:Cell Differentiation; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology*; D002999:Clone Cells; D006801:Humans; D008244:Lysophosphatidylcholines / Q000032:analysis* / Q000378:metabolism; D008246:Lysophospholipids / Q000032:analysis* / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000737:chemistry* / Q000166:cytology
D008244:Lysophosphatidylcholines; D008246:Lysophospholipids; C008301:lysophosphatidylethanolamine