Activity-guided isolation of antioxidants from the leaves of Terminalia arjuna.
An activity-guided isolation and purification process was used to identify the l,l-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging components of the food plant (Terminalia arjuna) of Antheraea mylitta. Dry leaves of T. arjuna were extracted with different solvents and tested for their antioxidant activity against DPPH(•). The acetone-water (8:2) extract expressed strong DPPH radical-scavenging activity, and was subjected to column chromatography over silica gel. Gallic acid, apigenin, luteolin, quercetin, epicatechin, ellagic acid and 1-O-β-galloyl glucose were isolated as active components and characterised by using different spectroscopic techniques.
a Bio-Organic Laboratory, Department of Chemistry , University of Delhi , Delhi 110007 , India.
Pradeep Pratap
PP
Singh
S M S
SM
Chauhan
D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D047310:Apigenin / Q000302:isolation & purification / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000494:pharmacology; D002392:Catechin / Q000302:isolation & purification / Q000494:pharmacology; D004610:Ellagic Acid / Q000302:isolation & purification / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005707:Gallic Acid / Q000302:isolation & purification / Q000494:pharmacology; D047348:Hydrolyzable Tannins / Q000302:isolation & purification / Q000494:pharmacology; D047311:Luteolin / Q000302:isolation & purification / Q000494:pharmacology; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010851:Picrates / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D011794:Quercetin / Q000302:isolation & purification / Q000494:pharmacology; D029766:Terminalia / Q000737:chemistry*
D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D047348:Hydrolyzable Tannins; D010851:Picrates; D010936:Plant Extracts; D004610:Ellagic Acid; C060474:glucogallin; D005707:Gallic Acid; D047310:Apigenin; D002392:Catechin; D011794:Quercetin; C004931:1,1-diphenyl-2-picrylhydrazyl; D047311:Luteolin
Antheraea mylitta; DPPH•; antioxidant; cardioprotective