Reconstitution of OmpF membrane protein on bended lipid bilayers: perforated hexagonal mesophases.
Membrane proteins have been reconstituted on lipid bilayers with zero mean-curvature (cubic phases or vesicles). Here we show that reconstitution of pore-forming membrane proteins can also occur on highly curved lipidic bilayers of reverse hexagonal mesophases, for which the mean-curvature is significantly different from zero. We further show that the membrane protein provides unique topological interconnectivities between the aqueous nanochannels, significantly enhancing mesophase transport properties.
Food & Soft Material Science, Institute of Food Nutrition and Health, ETH Zurich, Schmelzbergstrasse 9, LFO E23, 8092, Zurich, Switzerland. raffaele.mezzenga@hest.ethz.ch.
Alexandru
A
Zabara
Renata
R
Negrini
Patric
P
Baumann
Ozana
O
Onaca-Fischer
Raffaele
R
Mezzenga
D001692:Biological Transport; D008051:Lipid Bilayers / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry*; D008954:Models, Biological; D018272:Porins / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D008051:Lipid Bilayers; D008565:Membrane Proteins; C109237:OmpF protein; D018272:Porins; D014867:Water