Differentially expressed proteins in malignant and benign adrenocortical tumors.
We have compared the microsomal protein composition of eight malignant and six benign adrenocortical tumors with proteomic methods. IGF2 had increased level in the malignant tumors, confirming previous microarray studies on the same material. Aldolase A, a glycolytic enzyme, also showed increased levels in the malignant tissue compared to the benign. Additionally, several proteins belonging to complex I in the mitochondrial respiration chain showed decreased levels in the malignant tissue. Taken together, this may indicate a shift in energy metabolism where glycolysis may be favored over tight coupling of glycolysis and mitochondrial respiration, a phenomenon known as the Warburg effect. One of the complex I proteins that showed decreased levels in the malignant tissue was GRIM-19. This protein has been suggested as a tumor suppressive protein by being a negative regulator of STAT3. In summary, an analysis of the microsomal proteome in adrenocortical tumors identifies groups of proteins as well as specific proteins differentially expressed in the benign and malignant forms. These proteins shed light on the biology behind malignancy and could delineate future drug targets.
Rheumatology Unit, Department of Medicine, Karolinska Institutet, Stockholm, Sweden ; Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden ; Science for Life Laboratory, Cancer Proteomics Mass Spectrometry, Karolinska Institutet, Solna, Sweden.
Hanna
H
Kjellin
Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden ; Science for Life Laboratory, Cancer Proteomics Mass Spectrometry, Karolinska Institutet, Solna, Sweden.
Henrik
H
Johansson
Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden.
Anders
A
Höög
Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden ; Science for Life Laboratory, Cancer Proteomics Mass Spectrometry, Karolinska Institutet, Solna, Sweden.
Janne
J
Lehtiö
Rheumatology Unit, Department of Medicine, Karolinska Institutet, Stockholm, Sweden.
Per-Johan
PJ
Jakobsson
Department of Breast- and Endocrine Surgery, Section of Endocrine and Sarcoma Surgery, Karolinska University Hospital, Stockholm, Sweden.
Magnus
M
Kjellman
D000306:Adrenal Cortex Neoplasms / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D002853:Chromatography, Liquid; D004734:Energy Metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D007335:Insulin-Like Growth Factor II / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D040901:Proteomics; D021241:Spectrometry, Mass, Electrospray Ionization
D014408:Biomarkers, Tumor; D024101:Mitochondrial Proteins; D007335:Insulin-Like Growth Factor II