Metabolomic analysis of siderophore cheater mutants reveals metabolic costs of expression in uropathogenic Escherichia coli.
Bacterial siderophores are a group of chemically diverse, virulence-associated secondary metabolites whose expression exerts metabolic costs. A combined bacterial genetic and metabolomic approach revealed differential metabolomic impacts associated with biosynthesis of different siderophore structural families. Despite myriad genetic differences, the metabolome of a cheater mutant lacking a single set of siderophore biosynthetic genes more closely approximate that of a non-pathogenic K12 strain than its isogenic, uropathogen parent strain. Siderophore types associated with greater metabolomic perturbations are less common among human isolates, suggesting that metabolic costs influence success in a human population. Although different siderophores share a common iron acquisition function, our analysis shows how a metabolomic approach can distinguish their relative metabolic impacts in E. coli.
Center for Women's Infectious Diseases Research, Division of Infectious Diseases, Department of Medicine, Washington University School of Medicine , St. Louis, Missouri 63110, United States.
Haitao
H
Lv
Chia S
CS
Hung
Jeffrey P
JP
Henderson
D004758:Enterobactin / Q000031:analogs & derivatives / Q000096:biosynthesis; D048168:Escherichia coli K12 / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D007501:Iron / Q000378:metabolism*; D055442:Metabolome / Q000235:genetics*; D009154:Mutation; D017262:Siderophores / Q000096:biosynthesis; D056689:Uropathogenic Escherichia coli / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000472:pathogenicity; D014774:Virulence
D029968:Escherichia coli Proteins; D017262:Siderophores; D004758:Enterobactin; D007501:Iron