Short-term and continuing stresses differentially interplay with multiple hormones to regulate plant survival and growth.
The stress phytohormone, abscisic acid (ABA), plays important roles in facilitating plants to survive and grow well under a wide range of stress conditions. Previous gene expression studies mainly focused on plant responses to short-term ABA treatment, but the effect of sustained ABA treatment and their difference are poorly studied. Here, we treated plants with ABA for 1 h or 9 d, and our genome-wide analysis indicated the differentially regulated genes under the two conditions were tremendously different. We analyzed other hormones' signaling changes by using their whole sets of known responsive genes as reporters and integrating feedback regulation of their biosynthesis. We found that, under short-term ABA treatment, signaling outputs of growth-promoting hormones, brassinosteroids and gibberellins, and a biotic stress-responsive hormone, jasmonic acid, were significantly inhibited, while auxin and ethylene signaling outputs were promoted. However, sustained ABA treatment repressed cytokinin and gibberellin signaling, but stimulated auxin signaling. Using several sets of hormone-related mutants, we found candidates in corresponding hormonal signaling pathways, including receptors or transcription regulators, are essential in responding to ABA. Our findings indicate interactions of ABA-dependent stress signals with hormones at different levels are involved in plants to survive under transient stress and to adapt to continuing stressful environments.
a State Key Laboratory of Genetic Engineering, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, 200433, P.R. China.
Cangjing
C
Yang
Jingjing
J
Liu
Xinran
X
Dong
Zhenying
Z
Cai
Weidong
W
Tian
Xuelu
X
Wang
D000040:Abscisic Acid / Q000378:metabolism / Q000494:pharmacology*; D017360:Arabidopsis / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D060406:Brassinosteroids / Q000378:metabolism; D003583:Cytokinins / Q000378:metabolism; D005030:Ethylenes / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D007210:Indoleacetic Acids / Q000378:metabolism; D008954:Models, Biological; D020411:Oligonucleotide Array Sequence Analysis; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology*; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D013312:Stress, Physiological / Q000187:drug effects*; D016019:Survival Analysis; D013997:Time Factors
D029681:Arabidopsis Proteins; D060406:Brassinosteroids; D003583:Cytokinins; D005030:Ethylenes; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D018749:RNA, Plant; D000040:Abscisic Acid; C036216:ethylene
abscisic acid; continuing stress; crosstalk; phytohormone; short-term stress; survival and growth.