Plant Proteins Differently Affect Body Fat Reduction in High-fat Fed Rats.
This study examined the effects of corn gluten (CG), wheat gluten (WG), and soybean protein isolate (SPI), as well as their hydrolysates, on weight reduction in rats fed a high-fat diet. Eight-month-old male Sprague-Dawley rats (n=70) were fed a high-fat diet (40% of the calories were fat) for 4 weeks. Rats were then randomly divided into seven groups and were fed isocaloric diets with different protein sources for 8 weeks. The protein sources were casein (control group), intact CG (CG group), CG hydrolysate (CGH group), intact WG (WG group), WG hydrolysate (WGH group), intact SPI (SPI group), and SPI hydrolysate (SPIH group). Body weight gain, adipose tissue weights, lipid profiles in plasma and liver; and hepatic activities of carnitine palmitoyl transferase, fatty acid synthase (FAS), malic enzyme, and glucose-6-phosphate dehydrogenase were assessed. The CGH group showed significant weight reduction compared with the other groups. Epididymal fat pad and plasma triglycerides in the CGH group were the lowest and were significantly different than those in the control group. FAS activity in the CGH group was significantly lower than that in the other groups. In conclusion, the CGH diet of these experimental animals demonstrated a weight-reducing effect by lowering the adipose tissue weight and by affecting the activities of hepatic lipogenic enzymes.
Biofood Network, Ewha Womans University, Seoul 120-160, Korea ; Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 120-750, Korea.
Joohee
J
Kim
Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 120-750, Korea.
Hyo Jung
HJ
Lee
Department of Food Science and Technology, Seoul National University of Science and Technology, Seoul 139-743, Korea.
Ji Yeon
JY
Kim
Biofood Network, Ewha Womans University, Seoul 120-160, Korea ; Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 120-750, Korea.
Mi Kyung
MK
Kim
Biofood Network, Ewha Womans University, Seoul 120-160, Korea ; Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 120-750, Korea.
Oran
O
Kwon
corn gluten; hydrolysate; soybean protein isolate; weight reduction; wheat gluten