Preparative chromatography and natural products discovery.
Methodology is reviewed which significantly enhances the economics and efficiency of larger scale preparative normal phase chromatography. Maintenance of hydration of the silica media and regeneration and re-equilibration of the column after each separation is demonstrated to allow repeated use of the column without loss of performance or requirement of repacking.
Ironstone Separations, Inc., 147 County Road 245, Etta, MS 38627-9519, United States. Electronic address: jdmcchesney@yahoo.com.
James D
JD
McChesney
Ironstone Separations, Inc., 147 County Road 245, Etta, MS 38627-9519, United States.
Douglas L
DL
Rodenburg
D001688:Biological Products / Q000032:analysis*; D002845:Chromatography / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry; D012997:Solvents; D014867:Water / Q000737:chemistry
D001688:Biological Products; D012997:Solvents; D014867:Water; D012822:Silicon Dioxide