[Quantitative detection of APC/RASSF1A promoter methylation in the plasma of patients with cervical diseases].
OBJECTIVE
To quantitatively detect adenomatous polyposis coli(APC) and Ras association domain family 1A( RASSF1A) promoter methylation levels in the plasma of patients with cervical disease and to determine the diagnostic value of the indicators of cervical disease.


METHODS
Preoperative blood samples were collected from 25 cases of healthy women and 118 cases of cervical disease, and tissue samples were also collected from 31 cases of them. The APC/RASSF1A promoter methylation levels of plasma and tissue were determined by duplex real-time quantitative methylation specific PCR (qMSP).


RESULTS
Among 31 paired plasma and tissue samples, true negative rate of APC and RASSF1A genes were all 100%, and true positive rate of APC and RASSF1A genes were 3/5 and 7/9, respectively. In 143 cases of plasma samples, total positive rate of APC and (or) RASSF1A methylation was 3% (2/59) for control/low-grade lesions groups and 48% (40/84) for high-grade lesions/tumor groups (P < 0.01) . RASSF1A methylation rate was related to lymph node metastasis and International Federation of Gynecology and Obstetrics (FIGO) stage (P < 0.05).


CONCLUSIONS
The plasma APC/RASSF1A methylation detection may be with some application prospect in the diagnosis of cervical diseases.
Department of Laboratory Medicine, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Hong
H
Wang
Email: sypan@njmu.edu.cn.
Shi-yang
SY
Pan
Zhi-rui
ZR
Pang
Fang
F
Wang
Jian
J
Xu
Dan
D
Chen
Er-fu
EF
Xie
Li-xia
LX
Zhang
Li
L
Gao
Yu-qiao
YQ
Xu
Bai
B
Jin
D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood; D016022:Case-Control Studies; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000097:blood*; D005260:Female; D017491:Genes, APC; D006801:Humans; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction / Q000379:methods; D011401:Promoter Regions, Genetic; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D002578:Uterine Cervical Dysplasia / Q000097:blood / Q000175:diagnosis* / Q000235:genetics
D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; C411762:RASSF1 protein, human; D025521:Tumor Suppressor Proteins