Maintenance of strong morphological differentiation despite ongoing natural hybridization between sympatric species of Lomatia (Proteaceae).
OBJECTIVE
When species cohesion is maintained despite ongoing natural hybridization, many questions are raised about the evolutionary processes operating in the species complex. This study examined the extensive natural hybridization between the Australian native shrubs Lomatia myricoides and L. silaifolia (Proteaceae). These species exhibit striking differences in morphology and ecological preferences, exceeding those found in most studies of hybridization to date.


METHODS
Nuclear microsatellite markers (nSSRs), genotyping methods and morphometric analyses were used to uncover patterns of hybridization and the role of gene flow in morphological differentiation between sympatric species.


RESULTS
The complexity of hybridization patterns differed markedly between sites, however, signals of introgression were present at all sites. One site provided evidence of a large hybrid swarm and the likely presence of multiple hybrid generations and backcrosses, another site a handful of early generational hybrids and a third site only traces of admixture from a past hybridization event. The presence of cryptic hybrids and a pattern of morphological bimodality amongst hybrids often disguised the extent of underlying genetic admixture.


CONCLUSIONS
Distinct parental habitats and phenotypes are expected to form barriers that contribute to the rapid reversion of hybrid populations to their parental character state, due to limited opportunities for hybrid/intermediate advantage. Furthermore, strong genomic filters may facilitate continued gene flow between species without the danger of assimilation. Stochastic fire events facilitate temporal phenological isolation between species and may partly explain the bi-directional and site-specific patterns of hybridization observed. Furthermore, the findings suggest that F1 hybrids are rare, and backcrosses may occur rapidly following these initial hybridization events.
The Royal Botanic Gardens and Domain Trust, Sydney, New South Wales, Australia.
Emma J
EJ
McIntosh
Maurizio
M
Rossetto
Peter H
PH
Weston
Glenda M
GM
Wardle
D017753:Ecosystem; D051456:Gene Flow; D005838:Genotype; D006824:Hybridization, Genetic; D018895:Microsatellite Repeats; D009517:New South Wales; D018515:Plant Leaves / Q000033:anatomy & histology*; D039105:Proteaceae / Q000033:anatomy & histology* / Q000235:genetics*; D061350:Sympatry
Australia; Hybridization; Lomatia myricoides; Lomatia silaifolia; Proteaceae; admixture; fire; hybrid zone; introgression; microsatellite; morphometrics; species cohesion