Molecular and structural evaluation of dentin caries-like lesions produced by different artificial models.
This study evaluated structural and molecular issues of dentin caries-like lesions produced by different artificial models (ACL) compared with natural caries lesions (NCL). One hundred twenty-four sound occlusal dentin blocks and 47 carious blocks were obtained and surface hardness was analyzed (SH1). They were assigned to groups according to ACL: GB: Biological; GC: Chemical; GIS: In situ; GNC: natural caries (control). Blocks from groups 1, 2 and 3 were submitted to caries lesion induction. NCL and ACL blocks were submitted to surface hardness (SH 2), FT-Raman and µEDXRF analysis. All blocks were longitudinally sectioned and one of the halves was submitted to cross-sectional hardness (CSH) and the other to SEM analysis. SH1 and SH2 data were submitted to t test (unpaired and paired, respectively), CSH and SEM data to two-way and one-way ANOVA respectively, and Tukey and t tests, respectively (p<0.05). Data from FT-Raman/µEDXRF were submitted to one-way ANOVA and Dunnett multiple-comparisons test (a=0.05). GB and GNC showed lowest SH2 values that were significantly different from GC and GIS. Regarding CSH, GB and GNC showed no significant difference between them. SEM showed similar caries lesion depth for GB and GNC, being significantly higher than for GC and GIS. µEDXRF showed similar values of calcium and phosphate for GB and GNC; GNC values were significantly different from GIS. No significant difference was found among the groups concerning phosphate, carbonate and CH bonds values. For collagen type I, GC values were significantly different compared to other groups. It may be concluded that caries-like lesions produced by GB were the closest model to NCL.
Department of Pediatric Dentistry, Piracicaba School of Dentistry, UNICAMP - University of Campinas, PiracicabaSP, Brazil.
Larissa Ferreira
LF
Pacheco
Department of Pediatric Dentistry, Piracicaba School of Dentistry, UNICAMP - University of Campinas, PiracicabaSP, Brazil.
Éfani  Caroline de Freitas
É
Banzi
Department of Pediatric Dentistry, Piracicaba School of Dentistry, UNICAMP - University of Campinas, PiracicabaSP, Brazil.
Eliana
E
Rodrigues
Department of Dental Materials and Operative Dentistry, Vale do Paraíba University; School of Dentistry, Research and Development Institute, IP&D, Laboratory of Biomedical Vibrational Spectroscopy, LEVB. São José dos CamposSP, Brazil.
Luís Eduardo Silva
LE
Soares
Department of Pediatric Dentistry, Piracicaba School of Dentistry, UNICAMP - University of Campinas, PiracicabaSP, Brazil.
Fernanda Miori
FM
Pascon
Department of Restorative Dentistry, Dental Materials Division, Piracicaba School of Dentistry, UNICAMP - University of Campinas, PiracicabaSP, Brazil.
Lourenço
L
Correr-Sobrinho
Department of Pediatric Dentistry, Piracicaba School of Dentistry, UNICAMP - University of Campinas, PiracicabaSP, Brazil.
Regina Maria
RM
Puppin-Rontani
D003731:Dental Caries / Q000473:pathology*; D003743:Dental Enamel / Q000473:pathology; D003804:Dentin / Q000473:pathology*; D006244:Hardness; D006801:Humans; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D008964:Molar, Third; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties