Protective effects of 2',4'-dihydroxy-6'-methoxy-3',5'-dimethylchalcone against hydrogen peroxide-induced oxidative stress in hepatic L02 cell.
2',4'-Dihydroxy-6'-methoxy-3',5'-dimethylchalcone (DMC) is a chalcone isolated from the buds of Cleistocalyx operculatus (Roxb.) Merr. et Perry, and the hepatoprotective effects of DMC on Kunming mice have been studied in previous study. However, the effects of DMC on hepatocyte toxicity and corresponding mechanism remain unclear. The aim of this study was to evaluate the hepatoprotective mechanism of DMC in human hepatocytes (L02) treated with H₂O₂. The results demonstrated that pretreatment with DMC effectively protected H₂O₂-induced cell viability loss, cell membrane damage (lactate dehydrogenase, nitric oxide production and caspase-3 accumulation. Besides, DMC pretreatment increased the amount of glutathione, decreased malondialdehyde and the percentage of apoptotic L02 cells compared with only H₂O₂ treated group. Taken together, these results indicated that DMC had hepatoprotective effects against H₂O₂-induced liver injury by alleviating oxidative stress and apoptosis process in L02 cells, and DMC might be a potential candidate for the intervention of liver diseases.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, People's Republic of China.
Yue
Y
Lu
Yan-Yan
YY
Zhang
Ying-Chun
YC
Hu
Yan-Hua
YH
Lu
D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000737:chemistry / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D047188:Chalcones / Q000494:pharmacology*; D020834:Ethnopharmacology; D035264:Flowers / Q000737:chemistry / Q000254:growth & development; D005978:Glutathione / Q000819:agonists / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects*; D008516:Medicine, Chinese Traditional; D009569:Nitric Oxide / Q000378:metabolism; D009994:Osmolar Concentration; D016877:Oxidants / Q000037:antagonists & inhibitors / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects*; D027842:Syzygium / Q000737:chemistry / Q000254:growth & development
C501649:2',4'-dihydroxy-6'-methoxy-3',5'-dimethylchalcone; D000975:Antioxidants; D047188:Chalcones; D016877:Oxidants; D009569:Nitric Oxide; D006861:Hydrogen Peroxide; C505407:CASP3 protein, human; D053148:Caspase 3; D005978:Glutathione