CPPU elevates photosynthetic abilities, growth performances and yield traits in salt stressed rice (Oryza sativa L. spp. indica) via free proline and sugar accumulation.
Application of N-2-(chloro-4-pyridyl)-N-phenyl urea (CPPU) to salt susceptible cultivar of indica rice (cv. PTT1) effected on free proline and soluble sugar accumulation and regulated the physio-morphological responses when subjected to salt stress condition was firstly demonstrated in this study. Soluble sugars, including sucrose, glucose and fructose, in leaf blade and leaf sheath were enriched in 0.1 mM CPPU pretreated plants subsequently exposed to 16.6 dS m(-1) NaCl for 10 days. In the long period (15 days) salt stress, free proline content in the leaf blade and leaf sheath were evidently peaked to act as osmotic adjustment in the salt-stressed plants. In addition, the photosynthetic pigments, including chlorophyll a, chlorophyll b, total chlorophyll and total carotenoids, were maintained by the functional regulation of soluble sugar and free proline in the cellular levels, thereby leading to higher net photosynthetic rate. Further, the stomatal closure and transpiration rate in CPPU pretreated plants were retained under salt stress, thereby resulting in alleviation of growth performance and yield traits. This study suggested that exogenous application of CPPU may alternatively play effective role to improve the salt tolerant abilities of salt susceptible rice crop.
Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
Arega
A
Gashaw
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Pahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand.
Cattarin
C
Theerawitaya
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Pahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand.
Thapanee
T
Samphumphuang
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Pahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand. Electronic address: suriyanc@biotec.or.th.
Suriyan
S
Cha-um
Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand. Electronic address: kanyarat.sup@mahidol.ac.th.
Kanyaratt
K
Supaibulwatana
D050260:Carbohydrate Metabolism; D012275:Oryza / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D010671:Phenylurea Compounds / Q000494:pharmacology*; D010788:Photosynthesis / Q000187:drug effects*; D011392:Proline / Q000378:metabolism*; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D012965:Sodium Chloride / Q000378:metabolism*; D013312:Stress, Physiological / Q000187:drug effects
C000597774:N-(2-chloro-4-pyridyl)-N-phenylurea; D010671:Phenylurea Compounds; D012965:Sodium Chloride; D011392:Proline
Growth; Net photosynthetic rate; Osmotic adjustment; Photosynthetic pigment; Salt tolerance; Soluble sugar