Differential interferences of hemoglobin and hemolysis on insulin assay with the Abbott Architect-Ci8200 immunoassay.
OBJECTIVE
This paper evaluates the effect of hemoglobin (Hb) and hemolysis on insulin measurements with the Architect-Ci8200 analyzer.


METHODS
Insulin concentrations were measured using the Architect-Ci8200. Interference studies were performed by spiking serum pools of defined insulin concentrations with increasing concentrations of either free Hb or hemolysate. A change of more than 10% was taken as evidence of significant interference.


RESULTS
A significant negative bias in insulin results was observed only in samples spiked with hemolysate but not with free Hb. The bias was proportional to the degree of hemolysis and to the time elapsed before insulin assay. This interference was decreased when samples were kept at +4°C.


CONCLUSIONS
For all insulin requests, hemolysis must be systematically checked before biological interpretation of insulin results.
Department of Biochemistry, Hôpital Tenon, APHP, Paris F75020, France.
Simon
S
Garinet
Department of Biochemistry, Hôpital Tenon, APHP, Paris F75020, France; INSERM, U938, Faculté de Médecine Saint Antoine, ICAN, Institute of Cardiometabolism and Nutrition, F75012 Paris, France; UPMC Univ Paris 06, UMR_S 938, F75005 Paris, France.
Soraya
S
Fellahi
Department of Biochemistry, Hôpital Tenon, APHP, Paris F75020, France.
Ginette
G
Marlin
Department of Biochemistry, Hôpital Tenon, APHP, Paris F75020, France; INSERM, U938, Faculté de Médecine Saint Antoine, ICAN, Institute of Cardiometabolism and Nutrition, F75012 Paris, France; UPMC Univ Paris 06, UMR_S 938, F75005 Paris, France.
Jacqueline
J
Capeau
Department of Biochemistry, Hôpital Tenon, APHP, Paris F75020, France; INSERM, U938, Faculté de Médecine Saint Antoine, ICAN, Institute of Cardiometabolism and Nutrition, F75012 Paris, France; UPMC Univ Paris 06, UMR_S 938, F75005 Paris, France.
Guillaume
G
Lefèvre
Department of Biochemistry, Hôpital Tenon, APHP, Paris F75020, France; INSERM, U938, Faculté de Médecine Saint Antoine, ICAN, Institute of Cardiometabolism and Nutrition, F75012 Paris, France; UPMC Univ Paris 06, UMR_S 938, F75005 Paris, France. Electronic address: jean-philippe.bastard@tnn.aphp.fr.
Jean-Philippe
JP
Bastard
D006454:Hemoglobins / Q000378:metabolism*; D006461:Hemolysis; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods*; D007328:Insulin / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
D006454:Hemoglobins; D007328:Insulin
Hemoglobin; Hemolysis; Insulin