Role of P2X7 purinoceptors in neuroprotective mechanism of ischemic postconditioning in mice.
Cerebral ischemia-reperfusion (I-R) injury is one of the primary causes of ischemic stroke. Ischemic postconditioning (iPoCo) is evolving as an important adaptive technique to contain I-R injury. Some recent studies have shown neuroprotective effect of iPoCo. However, neuroprotective mechanism of iPoCo is not clear. So, the present study has been undertaken to investigate the possible role of P2X7 purinoceptors in neuroprotective mechanism of iPoCo in mice. Bilateral carotid artery occlusion for 12 min followed by R for 24 h produced a significant rise in cerebral infarct size and neurological severity score (NSS) along with impairment of memory and motor coordination. iPoCo, involving three episodes of 10-s carotid artery occlusion with intermittent R of 10 s applied just after ischemic insult of 12 min, produced a significant decrease in cerebral infarct size and NSS along with reversal of I-R-induced impairment of memory and motor coordination. iPoCo induced neuroprotective effects were significantly abolished by pretreatment with selective purinergic P2X7 receptor blocker Brilliant Blue G (40 mg/kg intraperitoneal). It may be concluded that neuroprotective effect of iPoCo probably involves in activation of purinergic P2X7 receptors.
Pharmacology Division, Department of Pharmaceutical Sciences and Drug Research, Faculty of Medicine, Punjabi University, Patiala, 147002, Punjab, India.
Chanpreet Singh
CS
Bindra
Amteshwar Singh
AS
Jaggi
Nirmal
N
Singh
D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D057775:Ischemic Postconditioning; D019194:Ischemic Preconditioning; D008568:Memory / Q000502:physiology; D051379:Mice; D009044:Motor Cortex / Q000473:pathology; D018696:Neuroprotective Agents; D058486:Receptors, Purinergic P2X7 / Q000378:metabolism*; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology
D018696:Neuroprotective Agents; D058486:Receptors, Purinergic P2X7