Highly efficient one-pot multienzyme (OPME) synthesis of glycans with fluorous-tag assisted purification.
Oligo(ethylene glycol)-linked light fluorous tags have been found to be optimal for conjugating to glycans for both high-yield enzymatic glycosylation reactions using one-pot multienzyme (OPME) systems and quick product purification using fluorous solid-phase extraction (FSPE) cartridges. The combination of OPME glycosylation systems and the FSPE cartridge purification scheme provides a highly effective strategy for facile synthesis and purification of glycans.
Department of Chemistry, University of California, One Shields Avenue, Davis, California 95616, USA. xiichen@ucdavis.edu.
Joel
J
Hwang
Hai
H
Yu
Hamed
H
Malekan
Go
G
Sugiarto
Yanhong
Y
Li
Jingyao
J
Qu
Van
V
Nguyen
Dongyuan
D
Wu
Xi
X
Chen
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005461:Fluorine / Q000737:chemistry*; D006031:Glycosylation; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry / Q000378:metabolism*; D011134:Polysaccharides / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D052616:Solid Phase Extraction
D009097:Multienzyme Complexes; D011134:Polysaccharides; D005461:Fluorine