Study of glucose biosensor lifetime improvement in 37°C serum based on PANI enzyme immobilization and PLGA biodegradable membrane.
Glucose sensors with long life span were fabricated and evaluated in 37°C serum to imitate in vivo conditions. Polyaniline nanofibers were electrodeposited on sensor electrodes for excessive glucose oxidase immobilization to extend the lifetime of sensors. The sensitivity of sensors stored in 37°C bovine serum was stable for 20 days without apparent descents, and still remained 45% of its initial value after 44 days. In order to further improve the life span of sensors, a degradable polymer film, poly lactic-co-glycolic acid (PLGA), was used as an out coating layer of glucose sensors. Results showed that the PLGA coated glucose sensors left 80% of its initial sensitivity after 44 days' storage in 37°C bovine serum. The sensors could be potentially used as long-term implantable devices for real time monitoring of glucose levels.
Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, China; Cyrus Tang Center for Sensor Materials and Applications, Zhejiang University, Hangzhou, China.
Lu
L
Fang
Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, China; Cyrus Tang Center for Sensor Materials and Applications, Zhejiang University, Hangzhou, China.
Bo
B
Liang
Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, China; Cyrus Tang Center for Sensor Materials and Applications, Zhejiang University, Hangzhou, China.
Guang
G
Yang
Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, China; Cyrus Tang Center for Sensor Materials and Applications, Zhejiang University, Hangzhou, China.
Yichuan
Y
Hu
Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, China; Cyrus Tang Center for Sensor Materials and Applications, Zhejiang University, Hangzhou, China.
Qin
Q
Zhu
Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, China; Cyrus Tang Center for Sensor Materials and Applications, Zhejiang University, Hangzhou, China. Electronic address: yexuesong@zju.edu.cn.
Xuesong
X
Ye
D000814:Aniline Compounds / Q000737:chemistry*; D000818:Animals; D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques / Q000295:instrumentation*; D001786:Blood Glucose / Q000032:analysis* / Q000378:metabolism; D001831:Body Temperature; D002417:Cattle; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D004867:Equipment Design; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D019344:Lactic Acid / Q000737:chemistry*; D008567:Membranes, Artificial; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer
D000814:Aniline Compounds; D001786:Blood Glucose; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; C416807:polyaniline; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D005949:Glucose Oxidase
Bovine serum; Glucose sensor; PANI; PLGA