Effects of typified propolis on mutans streptococci and lactobacilli: a randomized clinical trial.
OBJECTIVE
The aim of this study was to determine in a randomized, double-blind, placebo-controlled clinical trial the effects of typified propolis and chlorhexidine rinses on salivary levels of mutans streptococci (MS) and lactobacilli (LACT).


METHODS
One hundred patients were screened for salivary levels of MS >100,000 CFUs/mL of saliva. All patients presented with at least one cavitated decayed surface. Sixty patients met entry criteria. Subjects were adults 18-55 years old. After restoration of cavitated lesions patients were randomized to 3 experimental groups: 1) PROP-alcohol-free 2% typified propolis rinse (n = 20); 2) CHX- 0.12% chlorhexidine rinse; 3) PL-placebo mouthrinse. Patients rinsed unsupervised 15 mL of respective rinses twice a day for 1 min for 28 days. Patients were assessed for the salivary levels of MS (Dentocult SM) and LACT (Dentocult LB) at baseline, 7-day, 14-day, and at 28-day visits (experimental effects) and at 45-day visit (residual effects). General linear models were employed to analyze the data.


RESULTS
PROP was superior to CHX at 14-day and 28-day visits in suppressing the salivary levels of MS (p < .05). PROP was superior to PL at all visits (p < .01). The residual effects of PROP in suppressing the salivary levels of MS could still be observed at the 45-day visit, where significant differences between PROP and CHX (p < .05), were demonstrated. PROP was significantly superior than CHX in suppressing the levels of salivary LACT at the 28-day visit (p < .05).


CONCLUSIONS
Typified propolis rinse was effective in suppressing cariogenic infections in caries-active patients when compared to existing and placebo therapies.
Biomaterials Research Group - School of Dentistry - UNIBAN Bandeirante Anhanguera University - São Paulo - SP - Brazil.
Camillo
C
Anauate Netto
Professional Masters Program in Pharmacy - School of Pharmacy - UNIBAN Bandeirante Anhanguera University - São Paulo - SP - Brazil.
Maria Cristina
MC
Marcucci
Professional Masters Program in Pharmacy - School of Pharmacy - UNIBAN Bandeirante Anhanguera University - São Paulo - SP - Brazil.
Niraldo
N
Paulino
Biomaterials Research Group - School of Dentistry - UNIBAN Bandeirante Anhanguera University - São Paulo - SP - Brazil.
Andrea
A
Anido-Anido
Biomaterials Research Group - School of Dentistry - UNIBAN Bandeirante Anhanguera University - São Paulo - SP - Brazil.
Ricardo
R
Amore
Microbiology Research Group - Professional Masters Program in Pharmacy - UNIBAN Bandeirante Anhanguera University - São Paulo - SP - Brazil.
Sergio
S
de Mendonça
Biomaterials Research Group - School of Dentistry - UNIBAN Bandeirante Anhanguera University - São Paulo - SP - Brazil.
Laurindo
L
Borelli Neto
Department of Cariology & Comprehensive Care - College of Dentistry - New York University - New York-NY - USA.
Walter Antonio
WA
Bretz
Chlorhexidine; Lactobacilli; Mutans Streptococci; Randomized clinical trial; Typified propolis