Sensitive SERS glucose sensing in biological media using alkyne functionalized boronic acid on planar substrates.
In this work, we propose a novel glucose binding mechanism on a highly sensitive SERS substrate, in order to overcome challenges in specific glucose detection in bio-fluids. We make use of phenylboronic acid as a receptor for saccharide capture onto the substrate and the ability of the captured glucose molecule to undergo secondary binding with an alkyne-functionalized boronic acid to form a glucose-alkyne-boronic acid complex. The formation of this complex shows high selectivity for glucose, over other saccharides. In addition, the alkyne group of the alkyne-functionalized boronic acid exhibits a distinct Raman peak at 1996 cm(-1) in a biological silent region (1800-2800 cm(-1)) where most endogenous molecules, including glucose, show no Raman scattering, thus offering a high sensitivity over other SERS glucose sensing. The substrate offers long-term stability, as well as high SERS enhancement to the glucose-alkyne boronic acid complex on substrate. In addition, the reversibility of SERS signals at various incubation stages also shows reusability capabilities, whereas positive results in clinical urine samples demonstrate clinical feasibility. All these strongly suggest that this newly developed SERS-based assay offers great potential in glucose sensing.
Singapore Bioimaging Consortium, Agency for Science, Technology, and Research, Singapore.
Kien Voon
KV
Kong
Singapore Bioimaging Consortium, Agency for Science, Technology, and Research, Singapore.
Chris Jun Hui
CJ
Ho
Singapore Bioimaging Consortium, Agency for Science, Technology, and Research, Singapore.
Tianxun
T
Gong
Department of Urology, Singapore General Hospital, Singapore.
Weber Kam On
WK
Lau
Singapore Bioimaging Consortium, Agency for Science, Technology, and Research, Singapore; School of Physics, National University of Ireland, Galway, Ireland. Electronic address: Malini_Olivo@sbic.a-star.edu.sg.
Malini
M
Olivo
D000480:Alkynes / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D001897:Boronic Acids / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D006029:Glycosuria / Q000175:diagnosis*; D006801:Humans; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman / Q000379:methods*
D000480:Alkynes; D001897:Boronic Acids; D005947:Glucose; C010686:benzeneboronic acid
Alkyne; Biosensing; Boronic acid; Glucose; Surface-enhanced Raman spectroscopy