title: Rapid purification of monoclonal antibodies using magnetic microspheres.
abstract: Magnetic microspheres represent an interesting alternative to conventional chromatography resins in automated high-throughput protocols replacing centrifugation and filtration by magnetic separation. Some magnetic microspheres have unique features like high magnetite content and non-porous surface which allows them to migrate very fast in magnetic fields while binding target molecules with a low unspecific adsorption. Here, we describe the use of protein A or protein G-coated magnetic microspheres to purify quickly monoclonal antibodies from crude serum-free supernatants without the need of preliminary clarification or purification step. Using this method, multiple samples can be processed in parallel, a high level of purity can be obtained, and the purified IgG maintain their biological activity.
authors: NovImmune S.A., Plan-Les-Ouates, Switzerland.
authors: Pauline
authors: P
authors: Malinge
authors: Giovanni
authors: G
authors: Magistrelli
mesh_terms: D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000737:chemistry*; D008863:Microspheres; D013205:Staphylococcal Protein A / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; C034284:IgG Fc-binding protein, Streptococcus; D013205:Staphylococcal Protein A