title: Cloning, reformatting, and small-scale expression of monoclonal antibody isolated from mouse, rat, or hamster hybridoma.
abstract: The hybridoma technology, first described in 1975 by Milstein and Köhler, is still to date one of the most commonly used approaches to produce monoclonal antibodies. However, despite many advantages, this approach suffers from limitations like limited antibody productivity. Here, we describe a method for efficient cloning of antibody VH and VL produced by mouse, rat, or hamster hybridoma before reformatting in full-length IgG and small-scale expression in mammalian cell line.
authors: NovImmune S.A., Plan-Les-Ouates, Switzerland.
authors: Jeremy
authors: J
authors: Loyau
authors: François
authors: F
authors: Rousseau
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D006224:Cricetinae; D006825:Hybridomas / Q000378:metabolism*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000378:metabolism; D007147:Immunoglobulin Light Chains / Q000235:genetics / Q000378:metabolism; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000378:metabolism; D051379:Mice; D051381:Rats
chemical_list: D000911:Antibodies, Monoclonal; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region