title: Large-scale speciation of selenium in rice proteins using ICP-MS assisted electrospray MS/MS proteomics.
abstract: A Se-targeted bottom-up proteomics approach was developed for the identification of Se-containing proteins in rice grown naturally on seleniferous soils. The proteins were separated by 2D gel electrophoresis. The position of Se-containing spots was tentatively identified by the correlation between the 1D isoelectrofocusing (IEF) and 1D SDS electropherograms of a sample aliquot and confirmed by (78)Se imaging in the 2D gel. The method was complemented by the ICP-MS assisted shotgun proteomics approach. The proteins were identified by capHPLC with the dual ICP MS and electrospray Orbitrap MS detection. The first ever comprehensive study of rice selenoproteome revealed the presence of selenium, as both selenomethionine (SeMet) and selenocysteine (SeCys) residues, in a dozen proteins including a 19 kDa globulin, granule-bound starch synthase, and the family of glutelin-type seed storage proteins.
authors: CNRS/UPPA, Laboratoire de Chimie Analytique Bio-inorganique et Environnement (LCABIE), UMR5254, 2, av. Pr. Angot, 64053 Pau, France. joanna.szpunar@univ-pau.fr.
authors: Pracha
authors: P
authors: Cheajesadagul
authors: Juliusz
authors: J
authors: Bianga
authors: Carine
authors: C
authors: Arnaudguilhem
authors: Ryszard
authors: R
authors: Lobinski
authors: Joanna
authors: J
authors: Szpunar
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D008969:Molecular Sequence Data; D012275:Oryza / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D040901:Proteomics; D012643:Selenium / Q000032:analysis*; D017279:Selenocysteine / Q000032:analysis*; D012645:Selenomethionine / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D010940:Plant Proteins; D017279:Selenocysteine; D012645:Selenomethionine; D012643:Selenium