title: Carvacrol prevents methotrexate-induced renal oxidative injury and renal damage in rats.
abstract: OBJECTIVE
  The purpose of this study was to investigate the effect of carvacrol (CAR) on methotrexate (MTX)-induced renal damage in rats.
  
  
  METHODS
  Twenty-four male rats were equally divided into three groups: group I, control treatment; group II, MTX-treated; and group III, MTX+CAR-treated. A single dose of CAR (73 mg/kg) was administered intraperitoneally to group III on the first day of the experiment and a single dose of MTX (20 mg/kg) was administered intraperitoneally to groups II and III on the second day of the experiment. Blood samples and kidney tissue were obtained from each animal on day 8 for the measurement of malondialdehyde (MDA), total oxidant status (TOS), total antioxidant status (TAS), and oxidative stress index (OSI). Light microscopy was used for histopathological examination of kidney specimens.
  
  
  RESULTS
  MDA, TOS and OSI levels were significantly greater in the group receiving MTX alone relative to the control animals, while the TAS level was significantly reduced in the MTX group compared with the control group. The administration of CAR was associated with significantly decreased MDA, TOS, and OSI levels and increased TAS levels relative to the rats treated with MTX alone. Animals treated with CAR exhibited decreased tubular degeneration and architectural impairment relative to animals treated with MTX alone; however, the difference in histological scores did not meet the threshold of statistical significance.
  
  
  CONCLUSIONS
  MTX treatment results in oxidative damage to the rat kidney; damage which is partially abrogated by the administration of CAR.
authors: . drmehtapbozkurt@hotmail.com.
authors: Mehtap
authors: M
authors: Bozkurt
authors: Serda
authors: S
authors: Em
authors: Pelin
authors: P
authors: Oktayoglu
authors: Gul
authors: G
authors: Turkcu
authors: Hatice
authors: H
authors: Yuksel
authors: Mustafa A
authors: MA
authors: Sarıyıldız
authors: Mehmet
authors: M
authors: Caglayan
authors: Ibrahim
authors: I
authors: Batmaz
authors: Kemal
authors: K
authors: Nas
authors: Yasar
authors: Y
authors: Bozkurt
authors: Mahir
authors: M
authors: Kuyumcu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology; D000081007:Cymenes; D005493:Folic Acid Antagonists / Q000633:toxicity; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008727:Methotrexate / Q000633:toxicity*; D039821:Monoterpenes / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D000081007:Cymenes; D005493:Folic Acid Antagonists; D039821:Monoterpenes; D008315:Malondialdehyde; C073316:carvacrol; D008727:Methotrexate