title: Expression of PI3-K, PKB and GSK-3 β in the skeletal muscle tissue of gestational diabetes mellitus.
abstract: OBJECTIVE
  To analyze the expression of phosphatidylinositol 3 kinase (PI3-K), protein kinase B (PKB) and glycogen synthase kinase 3 beta (GSK-3 β) in skeletal muscle tissue of gestational diabetes mellitus (GDM).
  
  
  METHODS
  A total of 90 cases of pregnant women were divided into observation group and control group according to the occurrence of GDM with 45 cases in either, and the expression of PI3-K, PKB, GSK-3 β mRNA expression in skeletal muscle tissue was compared between two groups.
  
  
  RESULTS
  The total PI3-K p85 protein was significantly higher in the observation group compared with the control group, the activity of PI3-K was lower than that of the latter; The total PKB, GSK-3 β protein in skeletal tissue had no significant difference between two groups, while the serine phosphorylation levels of PKB and GSK-3β were significantly lower in observation group compared with the control group.
  
  
  CONCLUSIONS
  The downregulation of PI3-K, PKB and GSK-3βin skeletal tissue of GDM caused by phosphorylation dysfunction of signaling molecules is the reason for insulin resistance and transporter function decline which lead to GDM.
authors: Shaoxing Women and Children's Hospital, Shaoxing 312000, China.
authors: Tao
authors: T
authors: Zhang
authors: Shaoxing Women and Children's Hospital, Shaoxing 312000, China.
authors: Min
authors: M
authors: Fang
authors: Shaoxing Women and Children's Hospital, Shaoxing 312000, China.
authors: Zi-Mu
authors: ZM
authors: Fu
authors: Shaoxing Women and Children's Hospital, Shaoxing 312000, China.
authors: He-Chun
authors: HC
authors: Du
authors: Shaoxing Women and Children's Hospital, Shaoxing 312000, China.
authors: Hua
authors: H
authors: Yuan
authors: Shaoxing Women and Children's Hospital, Shaoxing 312000, China.
authors: Gui-Yu
authors: GY
authors: Xia
authors: Shaoxing Women and Children's Hospital, Shaoxing 312000, China.
authors: Jie
authors: J
authors: Feng
authors: Department of Gynaecology, Linyi People's Hospital, Linyi 276000, China. Electronic address: liuwg0539@163.com.
authors: Gui-Yun
authors: GY
authors: Yin
mesh_terms: D000328:Adult; D015331:Cohort Studies; D016640:Diabetes, Gestational / Q000201:enzymology / Q000378:metabolism*; D005260:Female; D038362:Glycogen Synthase Kinase 3 / Q000096:biosynthesis* / Q000737:chemistry; D000071679:Glycogen Synthase Kinase 3 beta; D006801:Humans; D007150:Immunohistochemistry; D018482:Muscle, Skeletal / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D058539:Phosphatidylinositol 3-Kinase / Q000096:biosynthesis* / Q000737:chemistry; D011247:Pregnancy; D051057:Proto-Oncogene Proteins c-akt / Q000096:biosynthesis* / Q000737:chemistry; D055815:Young Adult
chemical_list: D058539:Phosphatidylinositol 3-Kinase; C000605506:GSK3B protein, human; D000071679:Glycogen Synthase Kinase 3 beta; D051057:Proto-Oncogene Proteins c-akt; D038362:Glycogen Synthase Kinase 3
keywords: Gestational diabetes mellitus; Glycogen synthase kinase 3β; Phosphatidylinositol 3 kinase; Protein kinase B; Skeletal musclet