title: Molecularly imprinted cryogels for carbonic anhydrase purification from bovine erythrocyte.
abstract: Molecularly imprinted PHEMAH cryogels were synthesized and used for purification of carbonic anhydrase from bovine erythrocyte. Cryogels were prepared with free radical cryopolymerization of 2-hydroxyethyl methacrylate and methacryloylamido histidine and characterized by swelling degree, macroporosity, FTIR, SEM, surface area and elemental analysis. Maximum carbonic anhydrase adsorption of molecularly imprinted PHEMAH cryogel was found to be 3.16 mg/g. Selectivity of the molecularly imprinted cryogel was investigated using albumin, hemoglobin, IgG, γ-globulin, and lysozyme as competitor proteins and selectivity ratios were found to be 15.26, 60.05, 21.88, 17.61, and 17.42, respectively. Carbonic anhydrase purity was demonstrated by SDS-PAGE and zymogram results.
authors: Adnan Menderes University, Koçarlı Vocational and Training School , Aydın , Turkey.
authors: Murat
authors: M
authors: Uygun
authors: A Alev
authors: AA
authors: Karagözler
authors: Adil
authors: A
authors: Denizli
mesh_terms: D000327:Adsorption; D000818:Animals; D002256:Carbonic Anhydrases / Q000302:isolation & purification / Q000378:metabolism*; D002417:Cattle; D059985:Cryogels / Q000138:chemical synthesis*; D004912:Erythrocytes / Q000378:metabolism*; D005609:Free Radicals / Q000138:chemical synthesis; D006454:Hemoglobins / Q000737:chemistry; D006639:Histidine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry; D008689:Methacrylates / Q000138:chemical synthesis / Q000737:chemistry; D054802:Molecular Imprinting / Q000379:methods*; D009113:Muramidase / Q000737:chemistry; D058105:Polymerization; D011109:Polymethacrylic Acids / Q000138:chemical synthesis*; D011485:Protein Binding; D005719:gamma-Globulins / Q000737:chemistry
chemical_list: D059985:Cryogels; D005609:Free Radicals; D006454:Hemoglobins; D007074:Immunoglobulin G; D008689:Methacrylates; D011109:Polymethacrylic Acids; D005719:gamma-Globulins; C579323:poly(hydroxyethyl methacrylate-N-methacryloyl-histidine methyl ester); D006639:Histidine; C005044:hydroxyethyl methacrylate; D009113:Muramidase; D002256:Carbonic Anhydrases