title: Protective effect of fucoidan against AAPH-induced oxidative stress in zebrafish model.
abstract: Fucoidan, extracted from Ecklonia cava, has been extensively studied because of its wide biological activities. However, antioxidative activities have not been yet examined. Therefore we evaluated in vitro and in vivo studies on antioxidative activities of E. cava fucoidan (ECF). ECF exhibited more prominent effects in peroxyl radical scavenging activity, compared to the other scavenging activities. Thus, ECF was further evaluated for its protective ability against 2,2'-azobis dihydrochloride induced oxidative stress in Vero cells and ECF strongly reduced the AAPH-induced oxidative damage through scavenging intracellular reactive oxygen species. Furthermore, we evaluated protective effect of ECF against AAPH-induced oxidative stress in zebrafish model. ECF significantly reduced ROS generation, lipid peroxidation and cell death in zebrafish model. These findings indicate that ECF has antioxidant activities in vitro Vero cells and in vivo zebrafish model, even though ECF is not a polyphenol or flavonoid compound and does not contain benzene rings or conjugated structures.
authors: Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
authors: Eun-A
authors: EA
authors: Kim
authors: Division of Food Bioscience, Konkuk University, Chungju, Chungbuk 380-701, Republic of Korea.
authors: Seung-Hong
authors: SH
authors: Lee
authors: Research Institute of Processing from Jeju Fisher Food, Choung Ryong Fisheries Co., Ltd., Jeju 697-943, Republic of Korea.
authors: Chang-ik
authors: CI
authors: Ko
authors: Depatment of Pharmacology, School of Medicine, Ajou University Suwon, Republic of Korea.
authors: Seon-Heui
authors: SH
authors: Cha
authors: Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
authors: Min-Cheol
authors: MC
authors: Kang
authors: Pediatric Oncology Experimental Therapeutics Investigators Consortium (POETIC) Laboratory for Pre-Clinical and Drug Discovery Studies, University of Calgary, Calgary, AB, Canada.
authors: Sung-Myung
authors: SM
authors: Kang
authors: Department of Food and Nutrition, Sungshin Women's University, Seoul 136-742, Republic of Korea.
authors: Seok-Chun
authors: SC
authors: Ko
authors: Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
authors: Won-Woo
authors: WW
authors: Lee
authors: Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
authors: Ju-Young
authors: JY
authors: Ko
authors: Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
authors: Ji-Hyeok
authors: JH
authors: Lee
authors: Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
authors: Nalae
authors: N
authors: Kang
authors: Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
authors: Jae-Young
authors: JY
authors: Oh
authors: Laboratory of Comparative Animal Medicine, Division of Animal Life Science, Institute of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
authors: Ginnae
authors: G
authors: Ahn
authors: Department of Veterinary Medicine, Applied Radiological Science Research Institute, Jeju National University, Jeju 690-756, Republic of Korea.
authors: Young Heun
authors: YH
authors: Jee
authors: Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea. Electronic address: youjinj@jejunu.ac.kr.
authors: You-Jin
authors: YJ
authors: Jeon
mesh_terms: D000578:Amidines / Q000494:pharmacology*; D000818:Animals; D002522:Chlorocebus aethiops; D004578:Electron Spin Resonance Spectroscopy; D006321:Heart / Q000187:drug effects; D015227:Lipid Peroxidation; D023421:Models, Animal; D018384:Oxidative Stress / Q000187:drug effects*; D011134:Polysaccharides / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014709:Vero Cells; D015027:Zebrafish
chemical_list: D000578:Amidines; D011134:Polysaccharides; D017382:Reactive Oxygen Species; C046728:2,2'-azobis(2-amidinopropane); C007789:fucoidan
keywords: AAPH; Anti-oxidant; Fucoidan; Zebrafish