title: Stem cell responses to plasma surface modified electrospun polyurethane scaffolds.
abstract: The topographical effects from functional materials on stem cell behavior are currently of interest in tissue engineering and regenerative medicine. Here we investigate the influence of argon, oxygen, and hydrogen plasma surface modification of electrospun polyurethane fibers on human embryonic stem cell (hESC) and rat postnatal neural stem cell (NSC) responses. The plasma gases were found to induce three combinations of fiber surface functionalities and roughness textures. On randomly oriented fibers, plasma treatments lead to substantially increased hESC attachment and proliferation as compared to native fibers. Argon plasma was found to induce the most optimal combination of surface functionality and roughness for cell expansion. Contact guided migration of cells and alignment of cell processes were observed on aligned fibers. Neuronal differentiation around 5% was found for all samples and was not significantly affected by the induced variations of surface functional group distribution or individual fiber topography.
  
  
  BACKGROUND
  In this study the influence of argon, oxygen, and hydrogen plasma surface modification of electrospun polyurethane fibers on human embryonic stem cell and rat postnatal neural stem cell (NSC) responses is studied with the goal of clarifying the potential effects of functional materials on stem cell behavior, a topic of substantial interest in tissue engineering and regenerative medicine.
authors: Bionano Systems Laboratory, Department of Microtechnology and Nanoscience (MC2), Chalmers University of Technology, Göteborg, Sweden.
authors: Carl
authors: C
authors: Zandén
authors: Center for Brain Repair and Rehabilitation (CBR) Institute for Neuroscience and Physiology, Gothenburg, Sweden.
authors: Nina
authors: N
authors: Hellström Erkenstam
authors: Center for Brain Repair and Rehabilitation (CBR) Institute for Neuroscience and Physiology, Gothenburg, Sweden.
authors: Thomas
authors: T
authors: Padel
authors: Center for Brain Repair and Rehabilitation (CBR) Institute for Neuroscience and Physiology, Gothenburg, Sweden.
authors: Julia
authors: J
authors: Wittgenstein
authors: Bionano Systems Laboratory, Department of Microtechnology and Nanoscience (MC2), Chalmers University of Technology, Göteborg, Sweden; Key Laboratory of Advanced Display and System Applications & SMIT Center, Shanghai University, Mechatronical Engineering and Automation Building, Shanghai, People's Republic of China. Electronic address: johan.liu@chalmers.se.
authors: Johan
authors: J
authors: Liu
authors: Center for Brain Repair and Rehabilitation (CBR) Institute for Neuroscience and Physiology, Gothenburg, Sweden.
authors: H Georg
authors: HG
authors: Kuhn
mesh_terms: D057908:Argon Plasma Coagulation; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D011140:Polyurethanes / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000009:adverse effects / Q000737:chemistry*
chemical_list: D011140:Polyurethanes
keywords: Polyurethane; Scaffold; Stem cell; Surface modification