title: Photosensitizer loaded nano-graphene for multimodality imaging guided tumor photodynamic therapy.
abstract: Graphene, a 2-dimensional carbon nanomaterial, has attracted wide attention in biomedical applications, owing to its intrinsic physical and chemical properties. In this work, a photosensitizer molecule, 2-(1-hexyloxyethyl)-2-devinyl pyropheophorbide-alpha (HPPH or Photochlor®), is loaded onto polyethylene glycol (PEG)-functionalized graphene oxide (GO) via supramolecular π-π stacking. The obtained GO-PEG-HPPH complex shows high HPPH loading efficiency. The in vivo distribution and delivery were tracked by fluorescence imaging as well as positron emission tomography (PET) after radiolabeling of HPPH with (64)Cu. Compared with free HPPH, GO-PEG-HPPH offers dramatically improved photodynamic cancer cell killing efficacy due to the increased tumor delivery of HPPH. Our study identifies a role for graphene as a carrier of PDT agents to improve PDT efficacy and increase long-term survival following treatment.
authors: 1. Department of Radiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China ; 2. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, United States ; 3. State Key Laboratory for Powder Metallurgy, Central South University, Changsha, Hunan, 410083, China.
authors: Pengfei
authors: P
authors: Rong
authors: 2. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, United States ; 4. Institute of Functional Nano & Soft Materials (FUNSOM), Soochow University, Suzhou, Jiangsu 215123, China.
authors: Kai
authors: K
authors: Yang
authors: 2. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, United States.
authors: Avinash
authors: A
authors: Srivastan
authors: 2. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, United States.
authors: Dale O
authors: DO
authors: Kiesewetter
authors: 2. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, United States ; 5. Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, Fujian 361102, China.
authors: Xuyi
authors: X
authors: Yue
authors: 2. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, United States.
authors: Fu
authors: F
authors: Wang
authors: 2. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, United States ; 5. Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, Fujian 361102, China.
authors: Liming
authors: L
authors: Nie
authors: 2. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, United States.
authors: Ashwinkumar
authors: A
authors: Bhirde
authors: 2. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, United States.
authors: Zhe
authors: Z
authors: Wang
authors: 4. Institute of Functional Nano & Soft Materials (FUNSOM), Soochow University, Suzhou, Jiangsu 215123, China.
authors: Zhuang
authors: Z
authors: Liu
authors: 2. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, United States.
authors: Gang
authors: G
authors: Niu
authors: 1. Department of Radiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China.
authors: Wei
authors: W
authors: Wang
authors: 2. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, United States.
authors: Xiaoyuan
authors: X
authors: Chen
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival; D002734:Chlorophyll / Q000031:analogs & derivatives / Q000737:chemistry / Q000627:therapeutic use; D003301:Copper Radioisotopes / Q000627:therapeutic use; D006108:Graphite / Q000737:chemistry*; D051379:Mice; D008856:Microscopy, Fluorescence; D064847:Multimodal Imaging; D053758:Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use; D009369:Neoplasms / Q000188:drug therapy*; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000138:chemical synthesis / Q000627:therapeutic use*; D011092:Polyethylene Glycols / Q000737:chemistry; D049268:Positron-Emission Tomography
chemical_list: D003301:Copper Radioisotopes; D017319:Photosensitizing Agents; D002734:Chlorophyll; C083998:2-(1-hexyloxyethyl)-2-devinyl pyropheophorbide-a; D011092:Polyethylene Glycols; D006108:Graphite
keywords: Graphene oxide; HPPH; optical imaging.; photodynamic therapy; positron emission tomography