title: p42/p44 mitogen-activated protein kinases inhibit atrial natriuretic peptide mRNA transcription in gp130-mediated hypertrophic ventricular myocytes.
abstract: OBJECTIVE
  To understand the role of ANP mRNA transcription regulation in gp130-mediated cardiomyocyte hypertrophy, and the involved mitogen-activated protein kinase kinase (MEK)-extracellular signal-regulated kinase (ERK, also called p42/p44 MAPK) signaling pathway.
  
  
  METHODS
  Isolated neonatal ventricular myocytes were treated with different concentrations of CT-1 (10(-9), 10(-8)and 10(-7)mol/L). MTT was used to analyze the viability and RT-PCR was used to detect ANP mRNA levels in cardiomyocyte. To inhibit p42/p44 MAPK activity in hypertrophic cardiomyocytes, the cells were pretreated with a specific MEK1 inhibitor.
  
  
  RESULTS
  CT-1 significantly induced ANP mRNA expression and the viability of cardiomyocytes in a dose- and time-dependent manner. Furthermore, blocking p42/p44 MAPK activity by the special MEK1 inhibitor upregulated the ANP mRNA.
  
  
  CONCLUSIONS
  p42/p44 MAPK have an important role in suppressing ANP mRNA transcription and cell activity in gp130-mediated hypertrophic ventricular myocytes.
authors: Department of Physiology and Cardiovascular Institute, Hainan Medical College, Haikou 571199, PR China.
authors: Zhan-Ling
authors: ZL
authors: Dong
authors: Department of Physiology and Cardiovascular Institute, Hainan Medical College, Haikou 571199, PR China.
authors: Yang
authors: Y
authors: Wang
authors: Department of Physiology and Cardiovascular Institute, Hainan Medical College, Haikou 571199, PR China.
authors: Tian-Fa
authors: TF
authors: Li
authors: Department of Physiology and Cardiovascular Institute, Hainan Medical College, Haikou 571199, PR China.
authors: Shao-Jiang
authors: SJ
authors: Zheng
authors: Department of Physiology and Cardiovascular Institute, Hainan Medical College, Haikou 571199, PR China.
authors: Yue-Qiong
authors: YQ
authors: Kong
authors: Department of Physiology and Cardiovascular Institute, Hainan Medical College, Haikou 571199, PR China.
authors: You-Ling
authors: YL
authors: Lan
authors: Department of Physiology and Cardiovascular Institute, Hainan Medical College, Haikou 571199, PR China. Electronic address: guojl79@163.com.
authors: Jun-Li
authors: JL
authors: Guo
authors: Department of Physiology and Cardiovascular Institute, Hainan Medical College, Haikou 571199, PR China; Department of Physiology, Hainan Provincial Health School, Haikou 570311, PR China. Electronic address: fushigan@aliyun.com.
authors: Shi-Gan
authors: SG
authors: Fu
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D006332:Cardiomegaly / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D050822:Cytokine Receptor gp130 / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism / Q000494:pharmacology*; D006352:Heart Ventricles / Q000166:cytology; D020935:MAP Kinase Signaling System / Q000187:drug effects; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014158:Transcription, Genetic
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; D050822:Cytokine Receptor gp130; D009320:Atrial Natriuretic Factor; C092073:cardiotrophin 1; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3
keywords: Atrial natriuretic peptide; Cardiomyocytes; Hypertrophy