title: An immunomagnetic separation-real-time PCR system for the detection of Alicyclobacillus acidoterrestris in fruit products.
abstract: Alicyclobacillus acidoterrestris is the most important spoilage species within the Alicyclobacillus genus and has become a major issue in the pasteurized fruit juice industry. The aim of this study was to develop a method combining immunomagnetic separation (IMS) with real-time PCR system (IMS-PCR) for rapid and specific detection of A. acidoterrestris in fruit products. A real-time PCR with the TaqMan system was designed to target the 16S rDNA genes with specific primer and probe set. The specificity of the assay was confirmed using 9 A. acidoterrestris strains and 21 non-A. acidoterrestris strains. The results indicated that no combination of the designed primers and probe was found in any Alicyclobacillus genus except A. acidoterrestris. The detection limit of the established IMS-PCR was less than 10CFU/mL and the testing process was accomplished in 2-3h. For the three types of samples (sterile water, apple juice and kiwi juice), the correlation coefficient of standard curves was greater than 0.991, and the calculated PCR efficiencies were from 108% to 109%. As compared with the standard culture method performed concurrently on the same set of samples, the sensitivity, specificity and accuracy of IMS-PCR for 196 naturally contaminated fruit products were 90.0%, 98.3% and 97.5%, respectively. The results exhibited that the proposed IMS-PCR method was effective for the rapid detection of A. acidoterrestris in fruit products.
authors: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Zhouli
authors: Z
authors: Wang
authors: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Rui
authors: R
authors: Cai
authors: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Yahong
authors: Y
authors: Yuan
authors: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Chen
authors: C
authors: Niu
authors: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Zhongqiu
authors: Z
authors: Hu
authors: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: yuetl305@nwsuaf.edu.cn.
authors: Tianli
authors: T
authors: Yue
mesh_terms: D056450:Alicyclobacillus / Q000235:genetics / Q000502:physiology*; D001628:Beverages / Q000382:microbiology; D005516:Food Microbiology / Q000379:methods*; D005638:Fruit / Q000382:microbiology*; D018189:Immunomagnetic Separation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Alicyclobacillus acidoterrestris; Detection; Fruit products; Immunomagnetic separation; Real-time PCR