title: Protection of 4-hydroxybenzyl-chitooligomers against inflammatory responses in Chang liver cells.
abstract: The aim of this study was to investigate anti-inflammatory activity of 4-hydroxybenzyl-chitooligomers (HB-COS) in Chang liver cells stimulated by a cytokine mixture. It was revealed that HB-COS decreased the level of nitric oxide and prostaglandin E2 (PGE2) production by diminishing the expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) without significant cytotoxicity. Moreover, HB-COS exerted inhibitory effects on the production of pro-inflammatory mediator (interleukin-6) in Chang liver cells. Notably, HB-COS exhibited anti-inflammatory activities via blocking degradation of inhibitory kappa B alpha (IκB-α), translocation of nuclear factor kappa B (NF-κB), and phosphorylation of mitogen-activated protein kinases (MAPKs) in a dose-dependent manner. Collectively, these findings indicated that HB-COS possessed potential anti-inflammatory effects in Chang liver cells, and could be a useful therapeutic agent for the treatment of hepatic inflammatory diseases.
authors: Department of Biochemistry, Faculty of Biology, University of Science, VietNam National University Ho Chi Minh City (VNU-HCM), Ho Chi Minh City, Viet Nam.
authors: Mai Duy Luu
authors: MD
authors: Trinh
authors: Agricultural Hi-Tech Park of Ho Chi Minh City, Ho Chi Minh City, Viet Nam.
authors: Minh-Hiep
authors: MH
authors: Dinh
authors: Specialized Graduate School Science and Technology Convergence, Department of Marine-bio Convergence Science, Pukyong National University, Busan 608-737, Republic of Korea.
authors: Dai-Hung
authors: DH
authors: Ngo
authors: Department of Biochemistry, Faculty of Biology, University of Science, VietNam National University Ho Chi Minh City (VNU-HCM), Ho Chi Minh City, Viet Nam.
authors: Dang-Khoa
authors: DK
authors: Tran
authors: Department of Biochemistry, Faculty of Biology, University of Science, VietNam National University Ho Chi Minh City (VNU-HCM), Ho Chi Minh City, Viet Nam.
authors: Quoc-Tuan
authors: QT
authors: Tran
authors: Specialized Graduate School Science and Technology Convergence, Department of Marine-bio Convergence Science, Pukyong National University, Busan 608-737, Republic of Korea.
authors: Thanh-Sang
authors: TS
authors: Vo
authors: Department of Biochemistry, Faculty of Biology, University of Science, VietNam National University Ho Chi Minh City (VNU-HCM), Ho Chi Minh City, Viet Nam. Electronic address: nghiep75@yahoo.com.
authors: Dai-Nghiep
authors: DN
authors: Ngo
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D006801:Humans; D020672:I-kappa B Proteins / Q000378:metabolism; D007249:Inflammation / Q000188:drug therapy* / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects
chemical_list: D000893:Anti-Inflammatory Agents; D020672:I-kappa B Proteins; D015850:Interleukin-6; D016328:NF-kappa B; C000606204:NFKBIA protein, human; D000072000:NF-KappaB Inhibitor alpha; D009569:Nitric Oxide; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D020928:Mitogen-Activated Protein Kinases; D015232:Dinoprostone
keywords: 4-Hydroxybenzyl-chitooligomers; Anti-inflammation; Chang liver cells, iNOS, COX-2