title: Role of IL-31 in regulation of Th2 cytokine levels in patients with nasal polyps.
abstract: Interleukin-31 (IL-31) is reported to be involved in Th2 cell-mediated diseases. However, the regulatory effect of IL-31 in the pathogenesis of nasal polyps (NPs) has not been understood. This study is aimed to determine whether IL-31 production is associated with Th2 cytokine levels and clinical severity in patients with NPs. Thirty patients with NPs and fifteen normal controls were included, and IL-31 production was determined by immunohistochemistry and enzyme-linked immunosorbent assay, respectively. The relationship between IL-31 expression and clinical severity was also evaluated. Besides, the effect of IL-31 on Th2 cytokine expression by polyp epithelial cells was investigated. We observed significantly enhanced IL-31 mRNA and protein level expression in NPs compared with normal control. IL-31+ cells were found to be positively correlated with clinical severity of NPs. Furthermore, we provided the direct evidence that IL-31 up-regulates Th2 cytokines expressed by polyp epithelial cells. Our results demonstrate that enhanced Th2 cytokine levels was correlated with IL-31 expression in NPs and provide a possible explanation for IL-31's regulatory role in the pathogenesis of NPs.
authors: Department of Otolaryngology, RenHe Hospital, China Three Gorges University, No. 410, Yiling Road, Yichang, 443001, Hubei, China.
authors: Hong
authors: H
authors: Ouyang
authors: Jie
authors: J
authors: Cheng
authors: Yajun
authors: Y
authors: Zheng
authors: Jingdong
authors: J
authors: Du
mesh_terms: D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry; D007378:Interleukins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D009298:Nasal Polyps / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D018418:Th2 Cells / Q000378:metabolism*
chemical_list: C502187:IL31 protein, human; D007378:Interleukins; D012333:RNA, Messenger