title: Screening and identification of T helper 1 and linear immunodominant antibody-binding epitopes in spike 1 domain and membrane protein of feline infectious peritonitis virus.
abstract: Feline infectious peritonitis virus (FIP virus: FIPV) causes a fatal disease in wild and domestic cats. The development of an FIP-preventive vaccine requires an antigen that does not induce antibody-dependent enhancement, and T helper (Th)1 activity plays an important role in protect against FIPV infection. In the present study, we identified synthetic peptides including Th1 and a linear immunodominant antibody-binding epitope in the S1 domain and M protein of FIPV. We also identified peptides that strongly induce Th1 activity from those derived from the structural proteins (S, M, and N proteins) of FIPV based on this and previous studies (Satoh et al. [19]). No Th1 epitope-containing peptide was identified in the peptides derived from the S1 domain of type I FIPV. In contrast, 7 Th1 epitope-containing peptides were identified in the S1 domain of type II FIPV, and no linear immunodominant antibody-binding epitope was contained in any of these peptides. Eleven Th1 epitope-containing peptides common to each serotype were identified in the M protein-derived peptides, and 2 peptides (M-11 and M-12) contained the linear immunodominant antibody-binding epitope. Of the peptides derived from the S, M, and N proteins of FIPV, those that induced significantly stronger Th1 activity than that of the FIPV antigen were rescreened, and 4 peptides were identified. When 3 of these peptides (M-9, I-S2-15, and II-S1-24) were selected and administered with CpG-ODNs to SPF cats, M-9 and II-S1-24 induced Th1 activity. Our results may provide important information for the development of a peptide-based vaccine against FIPV infection.
authors: Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan.
authors: Tomomi
authors: T
authors: Takano
authors: Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan.
authors: Hiroyuki
authors: H
authors: Morioka
authors: Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan.
authors: Kohji
authors: K
authors: Gomi
authors: Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan.
authors: Keisuke
authors: K
authors: Tomizawa
authors: Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan.
authors: Tomoyoshi
authors: T
authors: Doki
authors: Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan. Electronic address: hohdatsu@vmas.kitasato-u.ac.jp.
authors: Tsutomu
authors: T
authors: Hohdatsu
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000595:Amino Acid Sequence; D000818:Animals; D001666:Binding Sites, Antibody; D002415:Cats; D000086862:Coronavirus M Proteins; D016765:Coronavirus, Feline / Q000276:immunology*; D005260:Female; D016056:Immunodominant Epitopes / Q000276:immunology*; D008081:Liposomes / Q000008:administration & dosage / Q000276:immunology; D008297:Male; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage / Q000276:immunology; D064370:Spike Glycoprotein, Coronavirus / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D022223:Vaccines, Subunit / Q000276:immunology; D014763:Viral Matrix Proteins / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; C408982:CPG-oligonucleotide; D000086862:Coronavirus M Proteins; D016056:Immunodominant Epitopes; D008081:Liposomes; D009838:Oligodeoxyribonucleotides; D064370:Spike Glycoprotein, Coronavirus; D022223:Vaccines, Subunit; D014763:Viral Matrix Proteins
keywords: Coronavirus; Feline infectious peritonitis; Peptide-based vaccine; Th1