title: A benzophenanthridine alkaloid, chelerythrine induces apoptosis in vitro in a Dalton's lymphoma.
abstract: OBJECTIVE
  The aim of this study was to investigate the effect of chelerythrine on DL cell apoptosis in an in vitro experimental setup.
  
  
  METHODS
  For tumor model, spontaneous occurring T-cell lymphoma designated as Dalton's lymphoma (DL) was selected. Double staining, transmission electron microscope (TEM), fluorescence microscopy, Western blotting, Reverse Transcriptase-Polymerase Chain Reaction, and DNA fragmentation assay were used to detect heat shock factor 1 (HSF1) and hsp70 expression and PKC phosphorylation, and apoptotic characteristic of DL cells.
  
  
  RESULTS
  Chelerythrine exposure resulted in significant morphological alteration comparable to that of apoptosis. Furthermore, it was confirmed by fluorescence microscopy, TEM analysis, and DNA fragmentation assay that 10 μg/mL of chelerythrine is capable of inducing apoptosis in DL cells. The suppression in HSF1 expression and subsequent inhibition of hsp70 expression in chelerythrine-treated DL cells suggest that chelerythrine induces apoptosis in DL cells by inhibiting the expression of these cytoprotective proteins.
  
  
  CONCLUSIONS
  Chelerythrine is capable of inducing apoptosis DL cells in vitro and therefore, it could be useful in combating tumor growth and progression.
authors: Sanjay
authors: S
authors: Kumar
authors: Praveen
authors: P
authors: Deepak
authors: Sanjay
authors: S
authors: Kumar
authors: Pramod K
authors: PK
authors: Gautam
authors: Department of Zoology, Banaras Hindu University, Varanasi, India.
authors: Arbind
authors: A
authors: Acharya
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053119:Benzophenanthridines / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis; D000076249:Heat Shock Transcription Factors; D006801:Humans; D016399:Lymphoma, T-Cell / Q000188:drug therapy*; D010766:Phosphorylation / Q000187:drug effects; D011493:Protein Kinase C / Q000037:antagonists & inhibitors* / Q000378:metabolism; D014157:Transcription Factors / Q000096:biosynthesis
chemical_list: D000970:Antineoplastic Agents; D053119:Benzophenanthridines; D004268:DNA-Binding Proteins; C000619706:HSF1 protein, human; D018840:HSP70 Heat-Shock Proteins; D000076249:Heat Shock Transcription Factors; D014157:Transcription Factors; C016299:chelerythrine; D011493:Protein Kinase C