title: Epigenetic regulation of death of crayfish glial cells but not neurons induced by photodynamic impact.
abstract: Epigenetic processes are involved in regulation of cell functions and survival, but their role in responses of neurons and glial cells to oxidative injury is insufficiently explored. Here, we studied the role of DNA methylation and histone deacetylation in reactions of neurons and surrounding glial cells to photodynamic treatment that induces oxidative stress and cell death. Isolated crayfish stretch receptor consisting of a single mechanoreceptor neuron surrounded by glial cells was photosensitized with aluminum phthalocyanine Photosens that induced neuron inactivation, necrosis of the neuron and glia, and glial apoptosis. Inhibitors of DNA methylation 5-azacytidine and 5-aza-2'-deoxycytidine (decitabine) reduced the level of PDT-induced necrosis of glial cells but not neurons by 1.3 and 2.0 times, respectively, and did not significantly influence apoptosis of glial cells. Histone deacetylase inhibitors valproic acid and trichostatin A inhibited PDT-induced both necrosis and apoptosis of satellite glial cells but not neurons by 1.6-2.7 times. Thus, in the crayfish stretch receptor DNA methylation and histone deacetylation are involved in epigenetic control of glial but not neuronal necrosis. Histone deacetylation also participates in glial apoptosis.
authors: A.B. Kogan Research Institute for Neurocybernetics, Southern Federal University, Rostov-on-Don 344090, Russia.
authors: S A
authors: SA
authors: Sharifulina
authors: Department of Biophysics and Biocybernetics, Southern Federal University, Rostov-on-Don 344090, Russia.
authors: M A
authors: MA
authors: Komandirov
authors: A.B. Kogan Research Institute for Neurocybernetics, Southern Federal University, Rostov-on-Don 344090, Russia; Department of Biophysics and Biocybernetics, Southern Federal University, Rostov-on-Don 344090, Russia. Electronic address: auzd@yandex.ru.
authors: A B
authors: AB
authors: Uzdensky
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000528:radiation effects; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics* / Q000502:physiology / Q000528:radiation effects; D003400:Astacoidea; D001374:Azacitidine / Q000031:analogs & derivatives / Q000494:pharmacology; D019175:DNA Methylation / Q000187:drug effects / Q000528:radiation effects; D000077209:Decitabine; D004791:Enzyme Inhibitors / Q000494:pharmacology; D044127:Epigenesis, Genetic / Q000187:drug effects / Q000528:radiation effects; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006877:Hydroxamic Acids / Q000494:pharmacology; D066298:In Vitro Techniques; D007211:Indoles / Q000494:pharmacology; D007834:Lasers; D008465:Mechanoreceptors / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects*; D009336:Necrosis / Q000139:chemically induced / Q000188:drug therapy / Q000235:genetics* / Q000503:physiopathology; D009457:Neuroglia / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects*; D009942:Organometallic Compounds / Q000494:pharmacology; D010775:Photic Stimulation / Q000009:adverse effects; D017319:Photosensitizing Agents / Q000494:pharmacology; D014635:Valproic Acid / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; D007211:Indoles; D009942:Organometallic Compounds; D017319:Photosensitizing Agents; C048577:aluminum tetrasulfophthalocyanine; C012589:trichostatin A; D014635:Valproic Acid; D000077209:Decitabine; D001374:Azacitidine
keywords: Cell death; DNA methylation; Epigenetic; Glia; Histone deacetylation; Neuron; Photodynamic