title: Analysis of microdialysate monoamines, including noradrenaline, dopamine and serotonin, using capillary ultra-high performance liquid chromatography and electrochemical detection.
abstract: Electrochemical methods are very often used to detect catecholamine and indolamine neurotransmitters separated by conventional reverse-phase high performance liquid chromatography (HPLC). The present paper presents the development of a chromatographic method to detect monoamines present in low-volume brain dialysis samples using a capillary column filled with sub-2μm particles. Several parameters (repeatability, linearity, accuracy, limit of detection) for this new ultrahigh performance liquid chromatography (UHPLC) method with electrochemical detection were examined after optimization of the analytical conditions. Noradrenaline, adrenaline, serotonin, dopamine and its metabolite 3-methoxytyramine were separated in 1μL of injected sample volume; they were detected above concentrations of 0.5-1nmol/L, with 2.1-9.5% accuracy and intra-assay repeatability equal to or less than 6%. The final method was applied to very low volume dialysates from rat brain containing monoamine traces. The study demonstrates that capillary UHPLC with electrochemical detection is suitable for monitoring dialysate monoamines collected at high sampling rate.
authors: CNRS, UMR5292, Lyon Neuroscience Research Center, CMO Team, Lyon F-69000, France; University of Lyon 1, Villeurbanne F-69000, France.
authors: Barbara
authors: B
authors: Ferry
authors: INSERM, U1028, Lyon Neuroscience Research Center, NeuroDialyTics, Lyon F-69000, France.
authors: Elena-Patricia
authors: EP
authors: Gifu
authors: INSERM, U1028, Lyon Neuroscience Research Center, NeuroDialyTics, Lyon F-69000, France.
authors: Ioana
authors: I
authors: Sandu
authors: INSERM, U1028, Lyon Neuroscience Research Center, NeuroDialyTics, Lyon F-69000, France; CNRS, UMR5292, Lyon Neuroscience Research Center, BioRaN, Lyon, F-69000, France.
authors: Luc
authors: L
authors: Denoroy
authors: University of Lyon 1, Villeurbanne F-69000, France; INSERM, U1028, Lyon Neuroscience Research Center, NeuroDialyTics, Lyon F-69000, France; University of Lyon, F-69000, France. Electronic address: sandrine.parrot@univ-lyon1.fr.
authors: Sandrine
authors: S
authors: Parrot
mesh_terms: D000818:Animals; D015306:Biogenic Monoamines / Q000032:analysis* / Q000302:isolation & purification; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D055664:Electrochemical Techniques; D057230:Limit of Detection; D016014:Linear Models; D017551:Microdialysis / Q000379:methods*; D051381:Rats; D015203:Reproducibility of Results
chemical_list: D015306:Biogenic Monoamines
keywords: Electrochemical detection; Microdialysis; Monoamine neurotransmitter; Ultra high performance liquid chromatography