title: Electrochemical biosensor based on self-assembled monolayers modified with gold nanoparticles for detection of HER-3.
abstract: We have developed a new immunological biosensor for ultrasensitive quantification of human epidermal growth factor receptor-3(HER-3). In order to construct the biosensor, the gold electrode surface was layered with, hexanedithiol, gold nanoparticles, and cysteamine, respectively. Anti-HER-3 antibody was covalently attached to cysteamine by glutaraldehyde and used as a bioreceptor in a biosensor system for the first time by this study. Surface characterization was obtained by means of electrochemical impedance spectroscopy and voltammetry. The proposed biosensor showed a good analytical performance for the detection of HER-3 ranging from 0.2 to 1.4 pg mL(-1). Kramers-Kronig transform was performed on the experimental impedance data. Moreover, in an immunosensor system, the single frequency impedance technique was firstly used for characterization of interaction between HER-3 and anti-HER-3. Finally the presented biosensor was applied to artificial serum samples spiked with HER-3.
authors: Namık Kemal University, Faculty of Science and Arts, Chemistry Department, Biochemistry Division, Tekirdağ, Turkey.
authors: Mehmet Çetin
authors: MÇ
authors: Canbaz
authors: Namık Kemal University, Faculty of Science and Arts, Chemistry Department, Biochemistry Division, Tekirdağ, Turkey.
authors: Ciğdem Sayıklı
authors: CS
authors: Simşek
authors: Namık Kemal University, Faculty of Science and Arts, Chemistry Department, Biochemistry Division, Tekirdağ, Turkey. Electronic address: msezginturk@hotmail.com.
authors: Mustafa Kemal
authors: MK
authors: Sezgintürk
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D003543:Cysteamine / Q000737:chemistry; D058266:Dielectric Spectroscopy / Q000379:methods; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry*; D020893:Receptor, ErbB-3 / Q000032:analysis*
chemical_list: D055910:Antibodies, Immobilized; D003543:Cysteamine; D006046:Gold; D020893:Receptor, ErbB-3
keywords: Anti-HER-3; Biosensor; Cancer biomarkers; HER-3; Single frequency impedance