title: Liquid perfluorochemical-supported hybrid cell culture system for proliferation of chondrocytes on fibrous polylactide scaffolds.
abstract: CP5 bovine chondrocytes were cultured on biodegradable electrospun fibrous polylactide (PLA) scaffolds placed on a flexible interface formed between two immiscible liquid phases: (1) hydrophobic perfluorochemical (PFC) and (2) aqueous culture medium, as a new way of cartilage implant development. Robust and intensive growth of CP5 cells was achieved in our hybrid liquid-solid-liquid culture system consisting of the fibrous PLA scaffolds in contrast to limited growth of the CP5 cells in traditional culture system with PLA scaffold placed on solid surface. The multicellular aggregates of CP5 cells covered the surface of PLA scaffolds and the chondrocytes migrated through and overgrew internal fibers of the scaffolds. Our hybrid culture system simultaneously allows the adhesion of adherent CP5 cells to fibers of PLA scaffolds as well as, due to use of phase of PFC, enhances the mass transfer in the case of supplying/removing of respiratory gases, i.e., O2 and CO2. Our flexible (independent of vessel shape) system is simple, ready-to-use and may utilize a variety of polymer-based scaffolds traditionally proposed for implant development.
authors: Biotechnology and Bioprocess Engineering Division, Faculty of Chemical and Process Engineering, Warsaw University of Technology, Waryńskiego 1, 00-645, Warsaw, Poland, pilarek@ichip.pw.edu.pl.
authors: Maciej
authors: M
authors: Pilarek
authors: Iwona
authors: I
authors: Grabowska
authors: Ilona
authors: I
authors: Senderek
authors: Michał
authors: M
authors: Wojasiński
authors: Justyna
authors: J
authors: Janicka
authors: Katarzyna
authors: K
authors: Janczyk-Ilach
authors: Tomasz
authors: T
authors: Ciach
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D005466:Fluorocarbons / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D011091:Polyesters; D054457:Tissue Scaffolds
chemical_list: D005466:Fluorocarbons; D011091:Polyesters; C033616:poly(lactide)