title: Chymase activities and survival in endotoxin-induced human chymase transgenic mice.
abstract: We examined the effects of overexpressed human chymase on survival and activity in lipopolysaccharide (LPS)-treated mice. Human chymase transgenic (Tg) and wild-type C57BL/6 (WT) mice were treated with LPS (0.03, 0.1 and 0.3 mg/day; intraperitoneal) for 2 weeks. Treatment with 0.03 mg LPS did not affect survival in either WT or Tg mice. WT mice were not affected by 0.1 mg/day of LPS, whereas 25% of Tg mice died. Survival of mice treated with 0.3 mg/day of LPS was 87.5% and 0% in WT and Tg, respectively. LPS-induced increases in chymase activity in the heart and skin were significantly greater in Tg than WT mice. These data suggest a possible contribution of human chymase activation to LPS-induced mortality.
authors: 1. Department of Pharmacology, Kagawa University Medical School, Kagawa, Japan.
authors: Kazi
authors: K
authors: Rafiq
authors: 1. Department of Pharmacology, Kagawa University Medical School, Kagawa, Japan.
authors: Yu-Yan
authors: YY
authors: Fan
authors: 1. Department of Pharmacology, Kagawa University Medical School, Kagawa, Japan.
authors: Shamshad J
authors: SJ
authors: Sherajee
authors: 2. Teijin Institute for Bio-Medical Research, Teijin Pharma Ltd., Tokyo, Japan.
authors: Yoshimasa
authors: Y
authors: Takahashi
authors: 2. Teijin Institute for Bio-Medical Research, Teijin Pharma Ltd., Tokyo, Japan.
authors: Junji
authors: J
authors: Matsuura
authors: 2. Teijin Institute for Bio-Medical Research, Teijin Pharma Ltd., Tokyo, Japan.
authors: Naoki
authors: N
authors: Hase
authors: 3. Department of Gastroenterology and Neurology, Kagawa University Medical School, Kagawa, Japan.
authors: Hirohito
authors: H
authors: Mori
authors: 1. Department of Pharmacology, Kagawa University Medical School, Kagawa, Japan.
authors: Daisuke
authors: D
authors: Nakano
authors: 3. Department of Gastroenterology and Neurology, Kagawa University Medical School, Kagawa, Japan.
authors: Hideki
authors: H
authors: Kobara
authors: 1. Department of Pharmacology, Kagawa University Medical School, Kagawa, Japan.
authors: Hirofumi
authors: H
authors: Hitomi
authors: 3. Department of Gastroenterology and Neurology, Kagawa University Medical School, Kagawa, Japan.
authors: Tsutomu
authors: T
authors: Masaki
authors: 4. Department of Cardiovascular Diseases, Fukuoka University Chikushi Hospital, Fukuoka, Japan.
authors: Hidenori
authors: H
authors: Urata
authors: 1. Department of Pharmacology, Kagawa University Medical School, Kagawa, Japan.
authors: Akira
authors: A
authors: Nishiyama
mesh_terms: D000818:Animals; D053818:Chymases / Q000096:biosynthesis / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006801:Humans; D008070:Lipopolysaccharides / Q000633:toxicity; D008822:Mice, Transgenic; D009206:Myocardium / Q000201:enzymology*; D012867:Skin / Q000187:drug effects / Q000201:enzymology*; D013534:Survival
chemical_list: D008070:Lipopolysaccharides; D053818:Chymases
keywords: chymase activity and lipopolysaccharide; endotoxemia; human chymase transgenic mice