title: Cytotoxic and antimicrobial activities of endophytic fungi isolated from Bacopa monnieri (L.) Pennell (Scrophulariaceae).
abstract: BACKGROUND
  Endophytes, which reside in plant tissues, have the potential to produce novel metabolites with immense benefits for health industry. Cytotoxic and antimicrobial activities of endophytic fungi isolated from Bacopa monnieri (L.) Pennell were investigated.
  
  
  METHODS
  Endophytic fungi were isolated from the Bacopa monnieri. Extracts from liquid cultures were tested for cytotoxicity against a number of cancer cell lines using the MTT assay. Antimicrobial activity was determined using the micro dilution method.
  
  
  RESULTS
  22% of the examined extracts showed potent (IC50 of <20 μg/ml) cytotoxic activity against HCT-116 cell line. 5.5%, 11%, 11% of the extracts were found to be cytotoxic for MCF-7, PC-3, and A-549 cell lines respectively. 33% extracts displayed antimicrobial activity against at least one test organism with MIC value 10-100 μg/ml. The isolate B9_Pink showed the most potent cytotoxic activity for all the cell lines examined and maximum antimicrobial activity against the four pathogens examined which was followed by B19.
  
  
  CONCLUSIONS
  Results indicated the potential for production of bioactive agents from endophytes of Bacopa monnieri.
authors: Microbial Biotechnology Department, Indian Institute of Integrative Medicine, Jammu, India. meenusamiksha@rediffmail.com.
authors: Meenu
authors: M
authors: Katoch
authors: Gurpreet
authors: G
authors: Singh
authors: Sadhna
authors: S
authors: Sharma
authors: Nidhi
authors: N
authors: Gupta
authors: Payare Lal
authors: PL
authors: Sangwan
authors: Ajit Kumar
authors: AK
authors: Saxena
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D034681:Bacopa / Q000382:microbiology*; D001688:Biological Products / Q000494:pharmacology* / Q000627:therapeutic use; D015179:Colorectal Neoplasms / Q000188:drug therapy; D060026:Endophytes / Q000302:isolation & purification; D005658:Fungi / Q000302:isolation & purification; D045325:HCT116 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D061986:MCF-7 Cells; D009369:Neoplasms / Q000188:drug therapy*
chemical_list: D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D001688:Biological Products