title: A decadentate Gd(III)-coordinating paramagnetic cosolvent for protein relaxation enhancement measurement.
abstract: Solvent paramagnetic relaxation enhancement (sPRE) arises from random collisions between paramagnetic cosolvent and protein of interest. The sPRE can be readily measured, affording protein structure information. However, lack of an inert cosolvent probe may yield sPRE values that are not consistent with protein structure. Here we synthesized a new sPRE probe, triethylenetetraamine hexaacetate trimethylamide gadolinium, or Gd(III)-TTHA-TMA. With a total of 10 coordination sites, this paramagnetic cosovlent eliminates an inner-sphere water molecule that can otherwise transfer relaxation to protein through exchange. With the metal ion centered, the new probe is largely spherical with a radius of 4.0 Å, permitting accurate back calculation of sPRE. The effectiveness Gd(III)-TTHA-TMA as a sPRE probe was demonstrated on three well-studied protein systems.
authors: State Key Laboratory of Magnetic Resonance and Atomic Molecular Physics and Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, 430071, Hubei Province, China.
authors: Xin-Hua
authors: XH
authors: Gu
authors: Zhou
authors: Z
authors: Gong
authors: Da-Chuan
authors: DC
authors: Guo
authors: Wei-Ping
authors: WP
authors: Zhang
authors: Chun
authors: C
authors: Tang
mesh_terms: D004492:Edetic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D005682:Gadolinium / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D011506:Proteins / Q000737:chemistry* / Q000648:ultrastructure; D014867:Water / Q000737:chemistry
chemical_list: D011506:Proteins; D014867:Water; C026059:triethylenetetraminehexaacetic acid; D004492:Edetic Acid; D005682:Gadolinium