title: OpenSource lab-on-a-chip physiometer for accelerated zebrafish embryo biotests.
abstract: Zebrafish (Danio rerio) embryo assays have recently come into the spotlight as convenient experimental models in both biomedicine and ecotoxicology. As a small aquatic model organism, zebrafish embryo assays allow for rapid physiological, embryo-, and genotoxic tests of drugs and environmental toxins that can be simply dissolved in water. This protocol describes prototyping and application of an innovative, miniaturized, and polymeric chip-based device capable of immobilizing a large number of living fish embryos for real-time and/or time-lapse microscopic examination. The device provides a physical address designation to each embryo during analysis, continuous perfusion of medium, and post-analysis specimen recovery. Miniaturized embryo array is a new concept of immobilization and real-time drug perfusion of multiple individual and developing zebrafish embryos inside the mesofluidic device. The OpenSource device presented in this protocol is particularly suitable to perform accelerated fish embryo biotests in ecotoxicology and phenotype-based pharmaceutical screening.
authors: The OpenTech Factory, School of Applied Sciences, RMIT University, Melbourne, Australia.
authors: Jin
authors: J
authors: Akagi
authors: Department of Molecular Medicine and Pathology, School of Medical Sciences, University of Auckland, Auckland, New Zealand.
authors: Chris J
authors: CJ
authors: Hall
authors: Department of Molecular Medicine and Pathology, School of Medical Sciences, University of Auckland, Auckland, New Zealand.
authors: Kathryn E
authors: KE
authors: Crosier
authors: School of Engineering, University of Glasgow, Glasgow, United Kingdom.
authors: Jonathan M
authors: JM
authors: Cooper
authors: Department of Molecular Medicine and Pathology, School of Medical Sciences, University of Auckland, Auckland, New Zealand.
authors: Philip S
authors: PS
authors: Crosier
authors: The OpenTech Factory, School of Applied Sciences, RMIT University, Melbourne, Australia.
authors: Donald
authors: D
authors: Wlodkowic
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D046149:Embryo Culture Techniques; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D057446:Hydrodynamics; D056656:Lab-On-A-Chip Devices; D015027:Zebrafish / Q000196:embryology*
chemical_list: D004129:Dimethylpolysiloxanes
keywords: Lab-on-a-Chip; OpenSource; PDMS; bioassay; biotest; embryo; microfluidics; pharmacology; toxicology; zebrafish