title: Yeasts and hygienic-sanitary microbial indicators in water buffalo mozzarella produced and commercialized in Minas Gerais, Brazil.
abstract: The aim of this work was to study the yeast populations and the main hygienic-sanitary microbial indicators in water buffalo mozzarella produced and commercialized in Minas Gerais, Brazil. Forty-two water buffalo mozzarella samples were purchased from retail outlets in Belo Horizonte. In addition, five samples of consecutive starter cultures, curd before acidification, acidified curd and mozzarella were collected at an industry in the city of Oliveira. Only three of the five water samples analyzed were suitable for consumption according to Brazilian sanitary standards. Four milk samples were highly contaminated with fecal coliforms, and did not meet the minimal hygienic-sanitary standards according to Brazilian regulations. Only one sample of buffalo muzzarela purchased from retail outlets exceeded the limit for coagulase-positive Staphylococcus. Eleven samples showed counts of thermotolerant coliforms higher than 5 × 10(3) CFU.g(-1), but still lower than the maximum permitted by the Brazilian laws. Salmonella spp. and Listeria monocytogenes were not isolated. Debaryomyces hansenii, Candida lusitaniae and C. parapsilosis were the prevalent yeast species isolated from cheese. Among samples from the production stages, the acidified curd presented the highest numbers of yeasts, with C. catenulata being the most frequent species isolated. Some opportunistic yeast species such as C. guilliermondii, C. tropicalis, C. parapsilosis, C. lusitaniae, C. catenulata, C. rugosa and C. krusei occurred in the mozzarella cheese samples analyzed. The mozzarella cheese presented a low microbial load as compared to other cheese already studied, and the yeast biota included species typical of cheese and also opportunistic pathogens.
authors: Departamento de Microbiologia, ICB, CP. 486, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors: Susanne
authors: S
authors: Facchin
authors: Departamento de Microbiologia, ICB, CP. 486, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors: Anne C
authors: AC
authors: Barbosa
authors: Departamento de Microbiologia, ICB, CP. 486, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors: Luiz S
authors: LS
authors: Carmo
authors: Laboratório de Enterotoxinas. Fundação Ezequiel Dias, Belo Horizonte MG, Brazil.
authors: Maria Crisolita C
authors: MC
authors: Silva
authors: Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors: Afonso L
authors: AL
authors: Oliveira
authors: Laboratório de Microbiologia Ambiental e Biotecnologia. Campus Universitário de Palmas. Universidade Federal do Tocantins, Palmas, TO, Brazil.
authors: Paula B
authors: PB
authors: Morais
authors: Departamento de Microbiologia, ICB, CP. 486, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors: Carlos A
authors: CA
authors: Rosa
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification*; D058491:Bacterial Load; D001938:Brazil; D002020:Buffaloes; D015169:Colony Count, Microbial; D003611:Dairy Products / Q000382:microbiology*; D015003:Yeasts / Q000145:classification / Q000302:isolation & purification*
keywords: hygienic-sanitary microbial indicators; opportunistic yeast; water buffalo mozzarella; yeast diversity