title: Importance of biofilm formation and dipeptidyl peptidase IV for the pathogenicity of clinical Porphyromonas gingivalis isolates.
abstract: The ability of Porphyromonas gingivalis to cause adult periodontitis is determined by its arsenal of virulence factors. Here, we investigated the importance of biofilm formation and bacterial dipeptidyl peptidase IV (DPPIV) for the pathogenicity of clinical P. gingivalis isolates. In our study, the isolates with biofilm-forming capacity also showed high DPPIV activity in vitro. Moreover, DPPIV activity increased in P. gingivalis biofilms compared to planktonic cells. In a murine subcutaneous abscess model, the biofilm-forming isolates with high DPPIV activity proved to be pathogenic, while the nonbiofilm formers with low DPPIV activity did not induce abscesses. The biofilm-forming ATCC 33277 strain with low DPPIV activity was not pathogenic in mice either. Our results suggest that biofilm formation and DPPIV activity contribute to the pathogenic potential of P. gingivalis. Furthermore, we show that biofilm formation may enhance P. gingivalis virulence through an increased DPPIV activity. Because of their importance for bacterial colonization and growth, biofilm formation and DPPIV activity could present interesting therapeutic targets to tackle periodontitis.
authors: Laboratory of Microbiology, Parasitology and Hygiene (LMPH), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Antwerp, Belgium.
authors: Sofie
authors: S
authors: Clais
authors: Gaëlle
authors: G
authors: Boulet
authors: Monique
authors: M
authors: Kerstens
authors: Tessa
authors: T
authors: Horemans
authors: Wim
authors: W
authors: Teughels
authors: Marc
authors: M
authors: Quirynen
authors: Ellen
authors: E
authors: Lanckacker
authors: Ingrid
authors: I
authors: De Meester
authors: Anne-Marie
authors: AM
authors: Lambeir
authors: Peter
authors: P
authors: Delputte
authors: Louis
authors: L
authors: Maes
authors: Paul
authors: P
authors: Cos
mesh_terms: D000038:Abscess / Q000382:microbiology; D000818:Animals; D018441:Biofilms / Q000254:growth & development*; D018819:Dipeptidyl Peptidase 4 / Q000096:biosynthesis*; D004195:Disease Models, Animal; D004789:Enzyme Activation; D005260:Female; D006801:Humans; D051379:Mice; D010518:Periodontitis / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000472:pathogenicity / Q000502:physiology*; D014774:Virulence
chemical_list: D018819:Dipeptidyl Peptidase 4
keywords: Porphyromonas gingivalis; biofilm; dipep-tidyl peptidase IV; in vitro; in vivo; virulence