title: Development of atom transfer radical polymer-modified gold nanoparticle-based enzyme-linked immunosorbent assay (ELISA).
abstract: In this work, a novel enzyme-linked immunosorbent assay (ELISA) with a low limit of detection and high sensitivity was developed using atom transfer radical polymer (ATRP)-modified gold nanoparticles (AuNPs). Clear signal amplification was achieved by introducing an abundance of horseradish peroxidase (HRP) to the AuNPs, because of the ATRP modification. This result suggested that the new ELISA was able to detect antigens in complex mixtures, and the limit of detection (LOD) was lower than that of conventional ELISA by a factor of 81. The new ELISA strategy greatly decreased the LOD during analysis and exhibited excellent reproducibility, stability, and feasibility. Therefore, it is a promising technique with many potential applications in biochemistry and medical science research.
authors: Training Department, Logistics University of Chinese Armed Police Forces , Tianjin 300309, People's Republic of China.
authors: Feng
authors: F
authors: Chen
authors: Shike
authors: S
authors: Hou
authors: Qingsheng
authors: Q
authors: Li
authors: Haojun
authors: H
authors: Fan
authors: Rong
authors: R
authors: Fan
authors: Zhongwei
authors: Z
authors: Xu
authors: Gahu
authors: G
authors: Zhala
authors: Xia
authors: X
authors: Mai
authors: Xiaoyi
authors: X
authors: Chen
authors: Xuyi
authors: X
authors: Chen
authors: Yingfu
authors: Y
authors: Liu
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D011108:Polymers / Q000737:chemistry*
chemical_list: D011108:Polymers; D006046:Gold; D006735:Horseradish Peroxidase