title: A structurally distinct human mycoplasma protein that generically blocks antigen-antibody union.
abstract: We report the discovery of a broadly reactive antibody-binding protein (Protein M) from human mycoplasma. The crystal structure of the ectodomain of transmembrane Protein M differs from other known protein structures, as does its mechanism of antibody binding. Protein M binds with high affinity to all types of human and nonhuman immunoglobulin G, predominantly through attachment to the conserved portions of the variable region of the κ and λ light chains. Protein M blocks antibody-antigen union, likely because of its large C-terminal domain extending over the antibody-combining site, blocking entry to large antigens. Similar to the other immunoglobulin-binding proteins such as Protein A, Protein M as well as its orthologs in other Mycoplasma species could become invaluable reagents in the antibody field.
authors: Department of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.|Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors: Rajesh K
authors: RK
authors: Grover
authors: Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors: Xueyong
authors: X
authors: Zhu
authors: Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors: Travis
authors: T
authors: Nieusma
authors: Department of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors: Teresa
authors: T
authors: Jones
authors: Department of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors: Isabel
authors: I
authors: Boreo
authors: Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors: Amanda S
authors: AS
authors: MacLeod
authors: Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors: Adam
authors: A
authors: Mark
authors: The Center for Physiological Proteomics, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors: Sherry
authors: S
authors: Niessen
authors: Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors: Helen J
authors: HJ
authors: Kim
authors: Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors: Leopold
authors: L
authors: Kong
authors: Synthetic Biology and Bioenergy Group, J Craig Venter Research Institute, Rockville, MD 20850, USA.
authors: Nacyra
authors: N
authors: Assad-Garcia
authors: Synthetic Biology and Bioenergy Group, J Craig Venter Research Institute, Rockville, MD 20850, USA.
authors: Keehwan
authors: K
authors: Kwon
authors: Comprehensive Cancer Center, Mayo Clinic Arizona, Scottsdale, AZ 85259, USA.
authors: Marta
authors: M
authors: Chesi
authors: Department of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors: Vaughn V
authors: VV
authors: Smider
authors: Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors: Daniel R
authors: DR
authors: Salomon
authors: Department of Internal Medicine, Division of Hematology, and Department of Immunology, Mayo Clinic, College of Medicine, Rochester, MN 55905, USA.
authors: Diane F
authors: DF
authors: Jelinek
authors: Department of Internal Medicine, Division of Hematology, and Department of Immunology, Mayo Clinic, College of Medicine, Rochester, MN 55905, USA.
authors: Robert A
authors: RA
authors: Kyle
authors: Departments of Pediatrics and Microbiology & Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.
authors: Richard B
authors: RB
authors: Pyles
authors: Synthetic Biology and Bioenergy Group, J Craig Venter Research Institute, Rockville, MD 20850, USA.
authors: John I
authors: JI
authors: Glass
authors: Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors: Andrew B
authors: AB
authors: Ward
authors: Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.|Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors: Ian A
authors: IA
authors: Wilson
authors: Department of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.|Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors: Richard A
authors: RA
authors: Lerner
mesh_terms: D000937:Antigen-Antibody Reactions / Q000235:genetics / Q000276:immunology*; D000941:Antigens / Q000276:immunology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D018360:Crystallography, X-Ray; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000276:immunology*; D007145:Immunoglobulin kappa-Chains / Q000276:immunology; D007146:Immunoglobulin lambda-Chains / Q000276:immunology; D008222:Lymphokines / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D009174:Mycoplasma / Q000276:immunology*; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology
chemical_list: D000941:Antigens; D001426:Bacterial Proteins; D007074:Immunoglobulin G; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D008222:Lymphokines; D008565:Membrane Proteins; D011994:Recombinant Proteins; C013059:immunoglobulin-binding factors