title: Inhibitor analysis and adaptive evolution of Saccharomyces cerevisiae for simultaneous saccharification and ethanol fermentation from industrial waste corncob residues.
abstract: Industrial waste corncob residues (CCR) are rich in cellulose and can be hydrolyzed directly without pretreatment. However, a poor fermentation performance was frequently observed in the simultaneous saccharification and ethanol fermentation (SSF) of CCR, although the furans and organic acid inhibitors were very low. In this study, the high level of water-insoluble phenolic compounds such as 2-furoic acid, ferulic acid, p-coumaric acid, guaiacol, and p-hydroxybenzoic acid were detected in CCR and inhibited the growth and metabolism of Saccharomyces cerevisiae DQ1. An evolutionary adaptation strategy was developed by culturing the S. cerevisiae DQ1 strain in a series of media with the gradual increase of CCR hydrolysate. The high ethanol concentration (62.68g/L) and the yield (55.7%) were achieved in the SSF of CCR using the adapted S. cerevisiae DQ1. The results provided a practical method for improving performance of simultaneous saccharification and ethanol production from CCR.
authors: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
authors: Hanqi
authors: H
authors: Gu
authors: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
authors: Jian
authors: J
authors: Zhang
authors: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China; State Key Laboratory of Motor Vehicle Biofuel Technology, Nanyang, Henan 473000, China. Electronic address: jbao@ecust.edu.cn.
authors: Jie
authors: J
authors: Bao
mesh_terms: D000222:Adaptation, Physiological; D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D008401:Gas Chromatography-Mass Spectrometry; D006868:Hydrolysis; D007220:Industrial Waste; D010636:Phenols / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D007220:Industrial Waste; D010636:Phenols; D000431:Ethanol
keywords: Corncob residues (CCR); Evolutionary adaptation; Saccharomyces cerevisiae DQ1; Simultaneous saccharification and ethanol fermentation (SSF); Water-insoluble phenolic inhibitors