title: Cellulose aerogel regenerated from ionic liquid solution for immobilized metal affinity adsorption.
abstract: Surface morphology of cellulosic adsorbents is expected to influence the adsorption behavior of biomacromolecules. In the present study, cellulose aerogel regenerated from ionic liquid solution was prepared for use as a polymer support for protein adsorption. Iminodiacetic acid groups were introduced to the aerogel for immobilized metal affinity adsorption of proteins. A Cu(II)-immobilized iminodiacetic acid cellulose aerogel (Cu(II)-IDA-CA), which has a large specific surface area, showed a higher adsorption capacity than Cu(II)-immobilized iminodiacetic acid bacterial cellulose (Cu(II)-IDA-BC) and Cu(II)-immobilized iminodiacetic acid plant cellulose (Cu(II)-IDA-PC). In contrast, the Cu(II)-immobilized cellulosic adsorbents showed similar adsorption capacities for smaller amino acid and peptides. The results show that cellulose aerogels are useful as polymer supports with high protein adsorption capacities.
authors: Department of Applied Chemistry, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan. Electronic address: oshimat@cc.miyazaki-u.ac.jp.
authors: Tatsuya
authors: T
authors: Oshima
authors: Department of Applied Chemistry, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan.
authors: Toshihiko
authors: T
authors: Sakamoto
authors: Department of Applied Chemistry, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan.
authors: Kaoru
authors: K
authors: Ohe
authors: Department of Applied Chemistry, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan.
authors: Yoshinari
authors: Y
authors: Baba
keywords: Adsorption; Cellulose aerogel; Immobilized metal affinity; Ionic liquids; Protein