title: Melatonin protects against behavioural dysfunctions and dendritic spine damage in 3-nitropropionic acid-induced rat model of Huntington's disease.
abstract: Huntington's disease (HD), an autosomal dominant neurodegenerative movement disorder in which striatal and cortical neurons are mostly affected, has no effective cure existing. A fungal neurotoxin and a potent inhibitor of mitochondrial electron transport chain complex II inhibitor, 3-nitropropionic acid (3-NP) is known to cause HD pathology, including lesions in the striatum and the cortex, and several behavioural syndromes in experimental animals. In the present study we examined the effect of melatonin on motor activities, neuronal morphology as revealed by Nissl and rapid Golgi staining, as well as GABA, glutamate and biogenic amine neurotransmitter levels in 3-NP-induced HD in rats. We found that melatonin (10, 20mg/kg, i.p.) administered 1h prior to 3-NP dose (20mg/kg; daily for 4 days) restored motor coordination ability as shown in gait, beam balancing, swim ability and performance on rotarod. However it failed to reduce 3-NP-induced striatal lesion core area, neuronal damage and the elevated levels of striatal dopamine. Melatonin administration partially restored 3-NP-induced loss of dendritic spines in the striatum and the cortex, and the reduction in cerebellar granule cell, but not hippocampal CA1 neuronal arborization. These findings collectively suggest that melatonin offers beneficial effects in correction of learning related fine motor adjustments, but not in behaviours unrelated to learning, by the restoration of striatal and cortical spines, and cerebellar granule cell arborization.
authors: Division of Cell Biology and Physiology, CSIR-Indian Institute of Chemical Biology, Laboratory of Clinical & Experimental Neuroscience, Rm # 117&119, Jadavpur, Kolkata 700032, India.
authors: J
authors: J
authors: Chakraborty
authors: Division of Cell Biology and Physiology, CSIR-Indian Institute of Chemical Biology, Laboratory of Clinical & Experimental Neuroscience, Rm # 117&119, Jadavpur, Kolkata 700032, India.
authors: D N
authors: DN
authors: Nthenge-Ngumbau
authors: Manovikas Kendra, Manovikas Biomedical Research and Diagnostic Centre, 482, Madudah, Plot I-24, Sector-J, E. M. Bypass, Kolkata 700107, India.
authors: U
authors: U
authors: Rajamma
authors: Division of Cell Biology and Physiology, CSIR-Indian Institute of Chemical Biology, Laboratory of Clinical & Experimental Neuroscience, Rm # 117&119, Jadavpur, Kolkata 700032, India. Electronic address: kpmohanakumar@yahoo.com.
authors: K P
authors: KP
authors: Mohanakumar
mesh_terms: D000818:Animals; D003292:Convulsants / Q000633:toxicity; D049229:Dendritic Spines / Q000187:drug effects* / Q000473:pathology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D006816:Huntington Disease / Q000139:chemically induced / Q000150:complications* / Q000473:pathology*; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D001523:Mental Disorders / Q000209:etiology / Q000473:pathology / Q000517:prevention & control; D009474:Neurons / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018377:Neurotransmitter Agents / Q000378:metabolism; D009574:Nitro Compounds / Q000633:toxicity; D011422:Propionates / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D016622:Silver Staining; D018709:Statistics, Nonparametric; D013997:Time Factors
chemical_list: D003292:Convulsants; D018696:Neuroprotective Agents; D018377:Neurotransmitter Agents; D009574:Nitro Compounds; D011422:Propionates; D008550:Melatonin; C015392:3-nitropropionic acid
keywords: Cerebellar granular cell arborization; Dendritic spine damage; Gait abnormality; Glutamate; Striatal biogenic amine neurotransmitters