title: Expression of β-catenin and mesenchymal markers in canine prostatic hyperplasia and carcinoma.
abstract: β-catenin is a nuclear signalling molecule that is associated with human prostatic neoplasia and the epithelial-mesenchymal transition (EMT) process. The present study evaluates immunohistochemically the expression of β-catenin and the mesenchymal markers vimentin, desmin, calponin and smooth muscle actin (SMA) in four normal canine prostates and prostate samples from 15 dogs with benign prostatic hyperplasia (BPH) and six with prostatic carcinoma (PC). β-catenin was located on the membrane of normal epithelial cells, while the same marker had both cytoplasmic and membrane expression in hyperplastic cells and a nuclear redistribution in PC. Vimentin-positive luminal cells were observed in two of the 15 cases of BPH and in all PC samples, suggesting the conversion of neoplastic epithelial cells to a mesenchymal type. SMA was consistently negative in PC, but there was mild desmin and calponin immunoreactivity in these lesions. As in men, β-catenin is involved in canine prostatic carcinogenesis, thus further validating the use of this animal model to study human prostatic disease.
authors: School of Veterinary Science, University of Queensland, Gatton Campus, Gatton 4343, Queensland, Australia.
authors: F Z X
authors: FZ
authors: Lean
authors: Hellenic Veterinary Laboratories S.A., 20th Km Leoforos Lavriou, 19002 Paiania Attikis, Greece.
authors: S
authors: S
authors: Kontos
authors: School of Veterinary Science, University of Queensland, Gatton Campus, Gatton 4343, Queensland, Australia. Electronic address: c.palmieri@uq.edu.au.
authors: C
authors: C
authors: Palmieri
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002277:Carcinoma / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D003893:Desmin / Q000378:metabolism; D004283:Dog Diseases / Q000378:metabolism* / Q000473:pathology; D004285:Dogs; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D058750:Epithelial-Mesenchymal Transition; D008297:Male; D008840:Microfilament Proteins / Q000378:metabolism; D011470:Prostatic Hyperplasia / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D014746:Vimentin / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism*; D000096985:Calponins
chemical_list: D000199:Actins; D014408:Biomarkers, Tumor; D002135:Calcium-Binding Proteins; D003893:Desmin; D008840:Microfilament Proteins; D014746:Vimentin; D051176:beta Catenin; D000096985:Calponins
keywords: dog; epithelial–mesenchymal transition; prostate; β-catenin