title: Biomimetic and cell-mediated mineralization of hydroxyapatite by carrageenan functionalized graphene oxide.
abstract: In bone tissue engineering, it is imperative to design multifunctional biomaterials that can induce and assemble bonelike apatite that is close to natural bone. In this study, graphene oxide (GO) was functionalized by carrageenan. The resulting GO-carrageenan (GO-Car) composite was further used as a substrate for biomimetic and cell-mediated mineralization of hydroxyapatite (HA). It was confirmed that carrageenan on the GO surface facilitated the nucleation of HA. The observation of the effect of the GO-Car on the adhesion, morphology, and proliferation of MC3T3-E1 cells was investigated. In vitro studies clearly show the effectiveness of GO-Car in promoting HA mineralization and cell differentiation. The results of this study suggested that the GO-Car hybrid will be a promising material for bone regeneration and implantation.
authors: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, ‡The Research Center of Biomedical Nanotechnology, and §Key Lab of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Lanzhou University , Lanzhou 730000, P. R. China.
authors: Hongyan
authors: H
authors: Liu
authors: Ju
authors: J
authors: Cheng
authors: Fengjuan
authors: F
authors: Chen
authors: Fengping
authors: F
authors: Hou
authors: Decheng
authors: D
authors: Bai
authors: Pinxian
authors: P
authors: Xi
authors: Zhengzhi
authors: Z
authors: Zeng
mesh_terms: D001672:Biocompatible Materials; D032701:Biomimetics; D018786:Bone Substitutes / Q000138:chemical synthesis* / Q000737:chemistry; D002351:Carrageenan / Q000737:chemistry*; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D017886:Durapatite / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D010006:Osteoblasts / Q000166:cytology*; D010087:Oxides / Q000737:chemistry*; D023822:Tissue Engineering / Q000295:instrumentation*
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D010087:Oxides; D006108:Graphite; D002351:Carrageenan; D017886:Durapatite