title: Biosynthesis of hygrocins, antitumor naphthoquinone ansamycins produced by Streptomyces sp. LZ35.
abstract: Hygrocins are naphthoquinone ansamycins with significant antitumor activities. Here, we report the identification and characterization of the hygrocin biosynthetic gene cluster (hgc) in Streptomyces sp. LZ35. A biosynthetic pathway is proposed based on bioinformatics analysis of the hgc genes and intermediates accumulated in selected gene disruption mutants. One of the steps during the biosynthesis of hygrocins is a Baeyer–Villiger oxidation between C5 and C6, catalyzed by luciferase- like monooxygenase homologue Hgc3. Hgc3 represents the founding member of a previously uncharacterized family of enzymes acting as Baeyer–Villiger monooxygenases.
authors: Shanren
authors: S
authors: Li
authors: Haoxin
authors: H
authors: Wang
authors: Yaoyao
authors: Y
authors: Li
authors: Jingjing
authors: J
authors: Deng
authors: Chunhua
authors: C
authors: Lu
authors: Yan
authors: Y
authors: Shen
authors: Yuemao
authors: Y
authors: Shen
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D047029:Lactams, Macrocyclic / Q000737:chemistry / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism; D005810:Multigene Family; D009285:Naphthoquinones / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D010802:Phylogeny; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*
chemical_list: D000970:Antineoplastic Agents; D001426:Bacterial Proteins; D047029:Lactams, Macrocyclic; D009285:Naphthoquinones; C506975:hygrocin A; C506976:hygrocin B; D006899:Mixed Function Oxygenases