title: Immune response to Newcastle disease virus vaccination in a wild passerine.
abstract: We studied the immune response of wild House Sparrows (Passer domesticus) experimentally challenged with different doses of inactivated Newcastle disease virus (NDV) vaccine. We evaluated within-individual cell-mediated and humoral responses in birds kept in outdoor aviaries, over a 6-wk period. Nonbreeding adult House Sparrows developed a significant humoral response to NDV experimental vaccination within 1 wk postchallenge, as measured by hemagglutination inhibition assay; values increased until week 4 and persisted until week 6. Differences among treatments appeared by week 1, with individuals challenged with the highest dose (0.2 mL) eliciting a higher humoral response than the rest (n = 18). By week 4, all individuals vaccinated displayed an increased humoral response, with individuals challenged with the highest dose remaining significantly above responses of individuals vaccinated with the middle dose (0.1 mL, n = 14), but not the lowest dose (0.05 mL, n = 15). The middle and lowest dose responded similarly and significantly different from controls (n = 23). Differences persisted through week 6 postchallenge. Cell-mediated responses were independent of the experimental treatment. All individuals experienced a rise in granulocyte concentration, whereas lymphocyte and monocyte concentrations decreased, most likely as a result of captivity. Adult wild House Sparrows immunochallenged with inactivated NDV vaccine developed a specific humoral response, highlighting the utility of this technique in immunologic and evolutionary ecology studies in wild birds.
authors: 1  Estación Biológica Doñana, CSIC, Avenida Americo Vespucio s/n, 41092 Sevilla, Spain.
authors: Juli
authors: J
authors: Broggi
authors: Olaya
authors: O
authors: García
authors: Francisco
authors: F
authors: Miranda
authors: Albert
authors: A
authors: Pagès
authors: Ramón C
authors: RC
authors: Soriguer
authors: Jordi
authors: J
authors: Figuerola
mesh_terms: D000818:Animals; D004306:Dose-Response Relationship, Immunologic; D009521:Newcastle Disease / Q000097:blood / Q000517:prevention & control*; D009522:Newcastle disease virus / Q000276:immunology*; D046370:Sparrows; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D014765:Viral Vaccines