title: An insulin-like growth factor found in hepatopancreas implicates carbohydrate metabolism of the blue crab Callinectes sapidus.
abstract: Hyperglycemia that is caused by the release of crustacean hyperglycemic hormone (CHH) from the sinus gland to hemolymph is one of the hallmark physiological phenomena, occurring in decapod crustaceans experiencing stressful conditions. However, the mechanism(s) by which such elevated glucose levels return to resting levels is still unknown. Interestingly, noted is a difference in the clearance rate of hemolymph glucose between adult females and adult males of the blue crab, Callinectes sapidus: the former with more rapid clearance than the latter. The presence of an endogenous-insulin-like molecule is suggested in C. sapidus because an injection of bovine insulin, significantly reduces the levels of hemolymph glucose that were previously elevated by emersion stress or the glucose injection. Using 5' and 3' RACE, the full-length cDNA of an insulin-like molecule is isolated from the hepatopancreas of an adult female C. sapidus and shows the same putative sequence of an insulin-like androgenic gland factor (IAG) but differs in 5' and 3' UTR sequences. A knock-down study using five injections of double-stranded RNA of CasIAG-hep (dsRNA-CasIAG-hep, 10μg/injection) over a 10-day period reduces CasIAG-hep expression by ∼50%. The levels of hemolymph glucose are also kept higher in dsRNA-CasIAG-hep injected group than those treated with dsRNA-green fluorescent protein (dsRNA-IAG-hep) or saline. Most importantly, the hepatopancreas of dsRNA-CasIAG-hep injected animals contains amounts of carbohydrate (glucose, trehalose, and glycogen) significantly lower than those of control groups, indicating that the function of CasIAG-hep in carbohydrate metabolism in crustaceans is similar to carbohydrate metabolism in vertebrates.
authors: University of Maryland Center for Environmental Science, Institute of Marine and Environmental Technology, 701 East Pratt Street, Columbus Center, Baltimore, MD, United States. Electronic address: chung@umces.edu.
authors: J Sook
authors: JS
authors: Chung
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D020121:5' Untranslated Regions / Q000235:genetics; D000375:Aging / Q000187:drug effects / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins; D001483:Base Sequence; D003386:Brachyura / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D050260:Carbohydrate Metabolism / Q000187:drug effects / Q000235:genetics; D002417:Cattle; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D020869:Gene Expression Profiling; D005947:Glucose / Q000378:metabolism; D006458:Hemolymph / Q000187:drug effects / Q000378:metabolism; D043143:Hepatopancreas / Q000187:drug effects / Q000378:metabolism*; D007101:Immersion; D007267:Injections; D007328:Insulin / Q000008:administration & dosage / Q000494:pharmacology; D007447:Invertebrate Hormones; D008297:Male; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D012330:RNA, Double-Stranded / Q000378:metabolism; D013002:Somatomedins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D060829:Arthropod Proteins; D018076:DNA, Complementary; D007328:Insulin; D007447:Invertebrate Hormones; D009419:Nerve Tissue Proteins; D012330:RNA, Double-Stranded; D013002:Somatomedins; C015947:hyperglycemic hormone, crustacean; D005947:Glucose
keywords: Callinectes sapidus; Carbohydrate metabolism; Hemolymph glucose; Hepatopancreas; Insulin-like androgenic gland factor; dsRNA-knock-down effect