title: [Adverse effect of environmental endocrine disruptors on gonadal development of prepubertal male rats and therapeutic effect of bushen tianjing recipe on it].
abstract: OBJECTIVE
  To verify the antagonistic effect of Bushen Tianjing Recipe (BTR) on environmental endocrine disruptors (EEDs) induced gonadal dysgenesis (GD) Sprague-Dawley (SD) male rat model.
  
  
  METHODS
  Totally 70 3-week-old male SD rats were randomly divided into seven groups, i.e., the control group (fed with corn oil), the model A group [di-2-ethylhexyl-phthalate (DEHP) 500 mg/kg], the CM A group (fed with DEHP 500 mg/kg + BTR 40 mL/kg), the exposed group B (fed with CYP 80 mg/kg), the CM B group (fed with CYP 80 mg/kg + BTR 40 mL/kg), the model C group [fed with DEHP 500 mg/kg + CYP 80 mL/kg], the CM C group (DEHP 500 mg/kg + CYP 80 mg/kg + BTR 40 mL/kg), respectively, 10 in each group. All were administered with corresponding medication by gastrogavage, once daily, for total 30 days. Rats were killed 24 h after the last administration, and their body weight and wet testis weight were weighed. The coefficient of testis was calculated. The serum testosterone (T) level was measured by chemiluminescent immunoassay. The histopathologic tissue was prepared. The ultrastructural changes of genital cells were observed by electron microscope.
  
  
  RESULTS
  Compared with the control group, there was no statistical difference in the body weight increase among all groups (P > 0.05). The time of testicular descent and preputial separation were significantly delayed in each exposed group (P < 0.01). In the exposed group A and the exposed group C, the wet weight of the testes was reduced and serum T level decreased (P < 0.01). The coefficient of testis significantly decreased in the exposed group A (P < 0.01). Compared with corresponding model group, the time of testicular descent and preputial separation were significantly fore-laid in each corresponding CM group (P < 0.01). The weight of the testes, the coefficient of testis, and the serum T level increased in the CM A group (P < 0.01). The serum T level obviously increased in the CM B group (P < 0.05).
  
  
  CONCLUSIONS
  The GD rat model was successfully duplicated by using DEHP. EEDs were proved to have significant anti-androgen activities. BTR was verified to have significant antagonistic to its anti-androgen effect.
authors: Department of Integrated Traditional and Western Medicine, Children's Hospital of Fudan University, Shanghai 200032, China.
authors: Xiang-Ting
authors: XT
authors: Li
authors: Department of Integrated Traditional and Western Medicine, Children's Hospital of Fudan University, Shanghai 200032, China.
authors: De-Pei
authors: DP
authors: Cai
mesh_terms: D000818:Animals; D004051:Diethylhexyl Phthalate / Q000633:toxicity; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D052244:Endocrine Disruptors / Q000633:toxicity*; D006059:Gonadal Dysgenesis / Q000139:chemically induced / Q000188:drug therapy*; D008297:Male; D008517:Phytotherapy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013739:Testosterone / Q000097:blood
chemical_list: D004365:Drugs, Chinese Herbal; D052244:Endocrine Disruptors; D013739:Testosterone; D004051:Diethylhexyl Phthalate