title: Determination of antibody structures.
abstract: While antibodies share a conserved structural framework, their complementarity-determining region loops are highly variable in size and sequence. Even more variable are the potential ways these loops can be used to interact with antigen. Thus, X-ray crystal structures of antibody Fab fragments and Fab-antigen complexes are critical for a detailed understanding of the antibody-antigen recognition process. This chapter describes the basic procedures necessary for the crystallization and structure determination of antibody Fab fragments by X-ray crystallography.
authors: Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA, USA.
authors: Robyn L
authors: RL
authors: Stanfield
mesh_terms: D000906:Antibodies / Q000737:chemistry* / Q000378:metabolism*; D018360:Crystallography, X-Ray; D007140:Immunoglobulin Fab Fragments
chemical_list: D000906:Antibodies; D007140:Immunoglobulin Fab Fragments