title: Saccharopolyspora indica sp. nov., an actinomycete isolated from the rhizosphere of Callistemon citrinus (Curtis).
abstract: A novel actinomycete strain, designated VRC122T, was isolated from a Callistemon citrinus rhizosphere sample collected from New Delhi, India, and its taxonomic status was determined by using a polyphasic approach. Strain VRC122T was a Gram-stain-positive, aerobic, non-motile, non-acid-alcohol-fast strain. Phylogenetic analysis based on 16S rRNA gene sequences showed the strain was placed in a well-separated sub-branch within the genus Saccharopolyspora. The highest levels of 16S rRNA gene sequence similarity were found with Saccharopolyspora hirsuta subsp. kobensis JCM 9109T (98.71%), Saccharopolyspora antimicrobica I05-00074T (98.69%) and Saccharopolyspora jiangxiensis W12T (98.66%); 16S rRNA gene sequence similarities with type strains of all other species of the genus Saccharopolyspora were below 98%. Chemosystematic studies revealed that it contained meso-diaminopimelic acid. Arabinose and galactose were the predominant whole-cell sugars. Diagnostic polar lipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylcholine. MK-9(H6) was the predominant menaquinone. C14:0, C16:0, iso-C15:0, iso-C16:0, iso-C17:0, anteiso-C15:0, anteiso-C17:0, C17:0 cyclo and summed feature 3 (C16:1ω7c and/or C16:1ω6c) were the major cellular fatty acids. The G+C content of the genomic DNA was 69.5 mol%. The results of DNA-DNA hybridization (30%, 22% and 25%, respectively) with type strains of the above-mentioned species, in combination with differences in physiological and biochemical data supported that strain VRC122T represents a novel species of the genus Saccharopolyspora, for which the name Saccharopolyspora indica sp. nov., is proposed. The type strain is VRC122T (=KCTC 29208T=MTCC 11564T=MCC 2206T=ATCC BAA-2551T).
authors: Department of Botany, Osmania University, Hyderabad-500007, Andhra Pradesh, India.
authors: Radha
authors: R
authors: Vaddavalli
authors: Department of pharmacy, Aditya Pharmaceutical College, Kakinada-533437, India.
authors: Sneha
authors: S
authors: Peddi
authors: Department of Microbiology, Osmania University, Hyderabad-500007, Andhra Pradesh, India.
authors: Srilekha Yadav
authors: SY
authors: Kothagauni
authors: Center for Cellular and Molecular Biology, Uppal Road, Hyderabad-500007, Andhra Pradesh, India.
authors: Zareena
authors: Z
authors: Begum
authors: Department of Botany, Osmania University, Hyderabad-500007, Andhra Pradesh, India.
authors: Bhagyanarayana
authors: B
authors: Gaddam
authors: Electron Microscope Facility, NIN, Tarnaka, Hyderabad-500007, Andhra Pradesh, India.
authors: Madhusudhanachary
authors: M
authors: Periketi
authors: Department of Microbiology, Osmania University, Hyderabad-500007, Andhra Pradesh, India.
authors: Venkateswar Rao
authors: VR
authors: Linga
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D007194:India; D008969:Molecular Sequence Data; D027822:Myrtaceae / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D016930:Saccharopolyspora / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9; D003960:Diaminopimelic Acid