title: Species identification through mitochondrial rRNA genetic analysis.
abstract: Inter-species and intraspecific variations in mitochondrial DNA (mtDNA) were observed in a bioinformatics analysis of the mitochondrial genomic sequences of 11 animal species. Some highly conserved regions were identified in the mitochondrial 12S and 16S ribosomal RNA (rRNA) genes of these species. To test whether these sequences are universally conserved, primers were designed to target the conserved regions of these two genes and were used to amplify DNA from 21 animal tissues, including two of unknown origin. By sequencing these PCR amplicons and aligning the sequences to a database of non-redundant nucleotide sequences, it was confirmed that these amplicons aligned specifically to mtDNA sequences from the expected species of origin. This molecular technique, when combined with bioinformatics, provides a reliable method for the taxonomic classification of animal tissues.
authors: 1] Division of Human Genetics, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229 [2] Department of Molecular & Cellular Physiology, College of Medicine, University of Cincinnati, 231 Albert Sabin Way, Cincinnati, OH 45267.
authors: Li
authors: L
authors: Yang
authors: Division of Human Genetics, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229.
authors: Zongqing
authors: Z
authors: Tan
authors: Center for Molecular and Human Genetics, Research Institute at Nationwide Children's Hospital, 700 Childrens Drive, Columbus, OH 43205.
authors: Daren
authors: D
authors: Wang
authors: 1] Division of Human Genetics, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229 [2] Attardi Institute of Mitochondrial Biomedicine, Wenzhou Medical College, Wenzhou, Zhejiang, China.
authors: Ling
authors: L
authors: Xue
authors: College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
authors: Min-Xin
authors: MX
authors: Guan
authors: 1] Division of Human Genetics, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229 [2] Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH 45229.
authors: Taosheng
authors: T
authors: Huang
authors: 1] Division of Human Genetics, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229 [2] Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH 45229.
authors: Ronghua
authors: R
authors: Li
mesh_terms: D000818:Animals; D001483:Base Sequence; D030541:Databases, Genetic; D006801:Humans; D008928:Mitochondria / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000032:analysis* / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000378:metabolism; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA
chemical_list: D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S; C075526:RNA, ribosomal, 12S