title: Genotoxicity of oxy-PAHs to Japanese medaka (Oryzias latipes) embryos assessed using the comet assay.
abstract: Polycyclic aromatic hydrocarbons (PAHs) have long been recognized as important environmental toxicants. Despite a plethora of information on the fate and effects of parent PAHs, relatively little is known about the environmental fate and toxicity of ketone- and quinone-substituted PAH oxidation products (termed oxy-PAHs), particularly in the aquatic environment. This study begins to fill that gap using embryos of the Japanese medaka (Oryzias latipes) as a model species. The genotoxic potential of two environmentally relevant oxy-PAHs, acenaphthenequinone and 7,12-benz[a]anthracenquinone, was assessed using the comet assay. We found that both oxy-PAHs could cause significant increases in DNA damage after only 48 h of exposure at the lowest concentrations tested (5 μg/L). Comparisons of the genotoxic potential between these oxy-PAHs and their corresponding parent PAHs (acenaphthene and benz[a]anthracene) and a well-known mutagenic PAH, benzo[a]pyrene, indicated similar potencies among all five of these compounds, particularly after longer (7 day) exposures. This study demonstrates the mutagenic potential of oxy-PAHs to an in vivo fish embryo model and points out the need for further study of their environmental occurrence and biologic effects.
authors: School of Marine and Atmospheric Sciences, Stony Brook University, Stony Brook, NY, 11794-5000, USA.
authors: Subham
authors: S
authors: Dasgupta
authors: Austin
authors: A
authors: Cao
authors: Brittany
authors: B
authors: Mauer
authors: Beizhan
authors: B
authors: Yan
authors: Seiichi
authors: S
authors: Uno
authors: Anne
authors: A
authors: McElroy
mesh_terms: D000818:Animals; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D009153:Mutagens / Q000633:toxicity; D009990:Oryzias / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism; D010084:Oxidation-Reduction; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000633:toxicity
chemical_list: D009153:Mutagens; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical