title: The Effect of miR-210 Up-regulation on Proliferation and Survival of Mouse Bone Marrow Derived Mesenchymal Stem Cell.
abstract: BACKGROUND
  Bone marrow derived mesenchymal stem cells (MSCs) are a population of multipotent progenitors which have the capacity of proliferation and differentiation into mesenchymal lineage cells. Hypoxia could promote the proliferation of MSCs. Micro-RNAs are endogenous RNAs that can play an important role in some processes such as proliferation and differentiation. MiR-210 could help for better proliferation of MSCs since this miRNA could activate HIF pathway. In current study we investigated if MSCs can preserve their differentiation and proliferation ability under normoxic conditions by upregulation of miR-210.
  
  
  METHODS
  MSCs isolated from C57 BL/6 mice by flushing it's femurs into the cell culture media. After 72 hours, MSCs which are plastic adherent cells were attached to the flask and non-adherent cells were removed. Subsequently, MSCs induced to differentiate into osteocytes and adipocytes with specific differentiation media in order to confirm their identity and multipotency. Then miR-210 was inserted in Lentiviruse vectors and affected MSCs. In each passage, the number and viability of cells were evaluated.
  
  
  RESULTS
  Comparison between miR-210 infected MSCs with control cells showed that miR-210 has ability to increase proliferation of MSCs significantly.
  
  
  CONCLUSIONS
  We showed that miR-210 has ability to induce proliferation of MSCs without any negative effect on their differentiation abilities. Further studies are needed for evaluation of probable effects of miR-210 mechanisms on MSCs proliferation.
authors: Hematology Department, Allied Medical School, Tehran University of Medical sciences, Tehran, Iran.
authors: Neda
authors: N
authors: Minayi
authors: Hematology Department, Allied Medical School, Tehran University of Medical sciences, Tehran, Iran.
authors: Shaban
authors: S
authors: Alizadeh
authors: Health care services Department, Medical School, Tehran University of Medical sciences, Tehran, Iran.
authors: Hosein
authors: H
authors: Dargahi
authors: Hematology Department, Tarbiat Modares University, Tehran, Iran.
authors: Masoud
authors: M
authors: Soleimani
authors: Hematology Department, Tarbiat Modares University, Tehran, Iran.
authors: Zahra Kashani
authors: ZK
authors: Khatib
authors: Hematology Department, Qaem Hospital, Mashad University of Medical sciences, Mashhad, Iran.
authors: Behnoosh
authors: B
authors: Tayebi
authors: Hematology Department, Tabriz University of Medical sciences, Tabriz, Iran.
authors: Mozhde
authors: M
authors: Mohammadian
authors: Hematology Department, Allied Medical School, Tehran University of Medical sciences, Tehran, Iran.
authors: Sadegh
authors: S
authors: Alijani
authors: Hematology Department, Allied Medical School, Tehran University of Medical sciences, Tehran, Iran.
authors: Farshid
authors: F
authors: Karami
keywords: HIF-1α; Mesenchymal stem cells; MiR-210