title: The crystal structure of novel chondroitin lyase ODV-E66, a baculovirus envelope protein.
abstract: Chondroitin lyases have been known as pathogenic bacterial enzymes that degrade chondroitin. Recently, baculovirus envelope protein ODV-E66 was identified as the first reported viral chondroitin lyase. ODV-E66 has low sequence identity with bacterial lyases at <12%, and unique characteristics reflecting the life cycle of baculovirus. To understand ODV-E66's structural basis, the crystal structure was determined and it was found that the structural fold resembled that of polysaccharide lyase 8 proteins and that the catalytic residues were also conserved. This structure enabled discussion of the unique substrate specificity and the stability of ODV-E66 as well as the host specificity of baculovirus.
authors: Laboratory of Structural Biology, Graduate School of System Life Sciences, Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
authors: Yoshirou
authors: Y
authors: Kawaguchi
authors: Institute for Molecular Science of Medicine, Aichi Medical University, 1-1 Yazakokarimata, Nagakute, Aichi 480-1195, Japan.
authors: Nobuo
authors: N
authors: Sugiura
authors: Research Complex for the Medicine Frontiers, Aichi Medical University, 1-1 Yazakokarimata, Nagakute, Aichi 480-1195, Japan.
authors: Koji
authors: K
authors: Kimata
authors: Laboratory of Structural Biology, Graduate School of System Life Sciences, Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan; Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
authors: Makoto
authors: M
authors: Kimura
authors: Laboratory of Structural Biology, Graduate School of System Life Sciences, Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan; Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan. Electronic address: kakuta@agr.kyushu-u.ac.jp.
authors: Yoshimitu
authors: Y
authors: Kakuta
keywords: Autographa californica; Chondroitin sulfate; Interdomain movement; ODV-E66; PL8; Polysaccharide lyase; occlusion derived virus envelope protein 66; polysaccharide lyase 8; β-Elimination