title: Dictyostelium cells migrate similarly on surfaces of varying chemical composition.
abstract: During cell migration, cell-substrate binding is required for pseudopod anchoring to move the cell forward, yet the interactions with the substrate must be sufficiently weak to allow parts of the cell to de-adhere in a controlled manner during typical protrusion/retraction cycles. Mammalian cells actively control cell-substrate binding and respond to extracellular conditions with localized integrin-containing focal adhesions mediating mechanotransduction. We asked whether mechanotransduction also occurs during non-integrin mediated migration by examining the motion of the social amoeba Dictyostelium discoideum, which is thought to bind non-specifically to surfaces. We discovered that Dictyostelium cells are able to regulate forces generated by the actomyosin cortex to maintain optimal cell-surface contact area and adhesion on surfaces of various chemical composition and that individual cells migrate with similar speed and contact area on the different surfaces. In contrast, during collective migration, as observed in wound healing and metastasis, the balance between surface forces and protrusive forces is altered. We found that Dictyostelium collective migration dynamics are strongly affected when cells are plated on different surfaces. These results suggest that the presence of cell-cell contacts, which appear as Dictyostelium cells enter development, alter the mechanism cells use to migrate on surfaces of varying composition.
authors: Department of Physics, University of Maryland College Park, College Park, Maryland, United States of America ; Laboratory of Cellular and Molecular Biology, Center for Cancer Research, NCI, NIH, Bethesda, Maryland, United States of America.
authors: Colin P
authors: CP
authors: McCann
authors: Department of Physics, University of Maryland College Park, College Park, Maryland, United States of America ; Laboratory of Cellular and Molecular Biology, Center for Cancer Research, NCI, NIH, Bethesda, Maryland, United States of America.
authors: Erin C
authors: EC
authors: Rericha
authors: Laboratory of Cellular and Molecular Biology, Center for Cancer Research, NCI, NIH, Bethesda, Maryland, United States of America ; Biophysics Graduate Program, University of Maryland, College Park, Maryland, United States of America.
authors: Chenlu
authors: C
authors: Wang
authors: Department of Physics, University of Maryland College Park, College Park, Maryland, United States of America ; Institute for Physical Science and Technology, University of Maryland, College Park, Maryland, United States of America.
authors: Wolfgang
authors: W
authors: Losert
authors: Laboratory of Cellular and Molecular Biology, Center for Cancer Research, NCI, NIH, Bethesda, Maryland, United States of America ; Institute for Physical Science and Technology, University of Maryland, College Park, Maryland, United States of America.
authors: Carole A
authors: CA
authors: Parent
mesh_terms: D000473:Alkanes / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects*; D016764:Cell Polarity / Q000187:drug effects; D004023:Dictyostelium / Q000166:cytology* / Q000187:drug effects; D005898:Glass / Q000737:chemistry*; D024462:Myosin Type II / Q000378:metabolism; D011107:Polylysine / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D012821:Silanes / Q000494:pharmacology*; D013499:Surface Properties; D016608:Talin / Q000378:metabolism
chemical_list: D000473:Alkanes; D012821:Silanes; D016608:Talin; C410437:tridecafluoro-1,1,2,2-tetrahydrooctyl-1-dimethylchlorosilane; D011107:Polylysine; D012710:Serum Albumin, Bovine; D024462:Myosin Type II