title: Rapid LC-MS-based metabolomics method to study the Fusarium infection of barley.
abstract: Ultra high performance liquid chromatography with quadrupole/time-of-flight mass spectrometry was applied to evaluate the potential of nontarget metabolomic fingerprinting in order to distinguish Fusarium-infected and control barley samples. First, the sample extraction and instrumental conditions were optimized to obtain the broadest possible representation of polar/medium-polar compounds occurring in extracts obtained from barley grain samples. Next, metabolomic fingerprints of extracts obtained from nine barley varieties were acquired under ESI conditions in both positive and negative mode. Each variety of barley was tested in two variants: artificially infected by Fusarium culmorum at the beginning of heading and a control group (no infection). In addition, the dynamics of barley infection development was monitored using this approach. The experimental data were statistically evaluated by principal component analysis, hierarchical clustering analysis, and orthogonal partial least-squares discriminant analysis. The differentiation of barley in response to F. culmorum infection was feasible using this metabolomics-based method. Analysis in positive mode provided a higher number of molecular features as compared to that performed under negative mode setting. However, the analysis in negative mode permitted the detection of deoxynivalenol and deoxynivalenol-3-glucoside considered as resistance-indicator metabolites in barley.
authors: Department of Food Analysis and Nutrition, Faculty of Food and Biochemical Technology, Institute of Chemical Technology, Prague, Czech Republic.
authors: Tomas
authors: T
authors: Cajka
authors: Marta
authors: M
authors: Vaclavikova
authors: Zbynek
authors: Z
authors: Dzuman
authors: Lukas
authors: L
authors: Vaclavik
authors: Jaroslava
authors: J
authors: Ovesna
authors: Jana
authors: J
authors: Hajslova
mesh_terms: D002851:Chromatography, High Pressure Liquid; D016000:Cluster Analysis; D005670:Fusarium / Q000502:physiology*; D001467:Hordeum / Q000378:metabolism* / Q000382:microbiology*; D016018:Least-Squares Analysis; D013058:Mass Spectrometry; D055432:Metabolomics; D010935:Plant Diseases / Q000382:microbiology*; D025341:Principal Component Analysis
keywords: Barley; Fusarium head blight; LC-MS/Metabolomics; Mycotoxins