title: Quantitative determination of cyclosporine in human whole blood by ultra-performance liquid chromatography with triple quadrupole tandem mass spectrometry.
abstract: Cyclosporine is an immunosuppressant drug used in organ transplants or for the treatment of autoimmune diseases. We developed and validated a simple, sensitive, and specific method using UPLC-MS/MS to determine cyclosporine levels in human whole blood. MS/MS detection was performed in the positive electrospray ionization mode with multiple reaction monitoring. Cyclosporine was extracted from whole-blood samples using ascomycin as an internal standard. The mass transitions m/z 1203.49 → 1185.53 and m/z 814.71 → 796.67 were used to assay the analyte and IS. This method was validated with respect to linearity, specificity, accuracy, precision, recovery, and stability. The method exhibited a linear response from 10 to 1000 ng mL(-1) with correlation coefficient values >0.99. The precision and the accuracy values were within 15%, except at the lower limit of quatification (LLOQ). Cyclosporine was stable in whole blood with no evidence of degradation. This method was successfully applied to a pharmacokinetic study of cyclosporine in healthy volunteers following oral administration.
authors: Department of Biomedical Science and Clinical Trial Center, Kyungpook National University Graduate School and Hospital.
authors: Hyun Jin
authors: HJ
authors: Jung
authors: Mi-Ri
authors: MR
authors: Gwon
authors: Jeonghyeon
authors: J
authors: Park
authors: Jeong Ju
authors: JJ
authors: Seo
authors: Sook Jin
authors: SJ
authors: Seong
authors: Eun Hee
authors: EH
authors: Kim
authors: Soon Rim
authors: SR
authors: Suh
authors: Ji Yun
authors: JY
authors: Jeong
authors: Hae Won
authors: HW
authors: Lee
authors: Young-Ran
authors: YR
authors: Yoon
mesh_terms: D000284:Administration, Oral; D053000:Analytic Sample Preparation Methods; D001774:Blood Chemical Analysis / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D016572:Cyclosporine / Q000008:administration & dosage / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics; D064368:Healthy Volunteers; D006801:Humans; D008297:Male; D053719:Tandem Mass Spectrometry; D013997:Time Factors
chemical_list: D016572:Cyclosporine