title: Establishment and validation of a model for non-luteinized human mural granulosa cell culture.
abstract: Cell culture techniques of human mural granulosa cells (MGCs) serve as a major in vitro tool. However, the use of luteinized MGCs has major limitations due to their luteinized state. Our aim was to establish a standardized protocol for the culture of MGCs as a model for different stages of folliculogenesis. We showed that early-non-luteinized, preovulatory-non-luteinized and luteal-MGCs have distinct gene expression pattern. After 4 days of incubation of luteinized-MGCs, ovulatory genes mRNA's achieve expression levels similar to the early non-luteinized follicles. FSH stimulation for 48 h of these 4 days cultured MGCs showed ovulatory genes mRNA's expression similar to the pre-ovulatory non-luteinized follicles. These FSH-stimulated cells responded to hCG stimulation in a pattern similar to the response of pre-ovulatory follicles. This novel model may provide a standardized research tool for delineation of the molecular processes occurring during the latter stages of follicular development in the human ovary.
authors: IVF Unit and Fertility Research Laboratory, Department of Obstetrics and Gynecology, Sheba Medical Center, Tel Hashomer 52621, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors: L
authors: L
authors: Ophir
authors: IVF Unit and Fertility Research Laboratory, Department of Obstetrics and Gynecology, Sheba Medical Center, Tel Hashomer 52621, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel. Electronic address: yuval.yung@sheba.health.gov.il.
authors: Y
authors: Y
authors: Yung
authors: IVF Unit and Fertility Research Laboratory, Department of Obstetrics and Gynecology, Sheba Medical Center, Tel Hashomer 52621, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors: E
authors: E
authors: Maman
authors: IVF Unit and Fertility Research Laboratory, Department of Obstetrics and Gynecology, Sheba Medical Center, Tel Hashomer 52621, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors: N
authors: N
authors: Rubinstein
authors: IVF Unit and Fertility Research Laboratory, Department of Obstetrics and Gynecology, Sheba Medical Center, Tel Hashomer 52621, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors: G M
authors: GM
authors: Yerushalmi
authors: IVF Unit and Fertility Research Laboratory, Department of Obstetrics and Gynecology, Sheba Medical Center, Tel Hashomer 52621, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors: J
authors: J
authors: Haas
authors: IVF Unit and Fertility Research Laboratory, Department of Obstetrics and Gynecology, Sheba Medical Center, Tel Hashomer 52621, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors: E
authors: E
authors: Barzilay
authors: IVF Unit and Fertility Research Laboratory, Department of Obstetrics and Gynecology, Sheba Medical Center, Tel Hashomer 52621, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors: A
authors: A
authors: Hourvitz
mesh_terms: D000328:Adult; D001141:Aromatase / Q000235:genetics / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002478:Cells, Cultured; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics / Q000378:metabolism; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D015870:Gene Expression; D006107:Granulosa Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D034461:Luteinization / Q000235:genetics*; D008954:Models, Biological; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D011966:Receptors, LHRH / Q000235:genetics / Q000378:metabolism
chemical_list: D015415:Biomarkers; D006063:Chorionic Gonadotropin; D012333:RNA, Messenger; D011966:Receptors, LHRH; D005640:Follicle Stimulating Hormone; D001141:Aromatase; C561410:CYP19A1 protein, human; D002786:Cholesterol Side-Chain Cleavage Enzyme
keywords: Cell culture; Follicle; Luteinization; Mural granulosa cells; Ovary