title: Tolerance of S. cerevisiae and Z. mobilis to inhibitors produced during dilute acid hydrolysis of soybean meal.
abstract: The objective of this research was to determine the minimum inhibitory concentration of 5-hydroxymethyl furfural, furfural, and acetic acid on Saccharomyces cerevisiae (NRRL Y-2233) and Zymomonas mobilis subspecies mobilis (NRRL B-4286) in both detoxified hydrolyzed soybean meal and synthetic YM broth spiked with the three compounds. Soybean meal was hydrolyzed with dilute sulfuric acid (0.0, 0.5, 1.25, and 2.0% wt v(-1)) at three temperatures (105, 120, and 135°C) and three durations (15, 30, and 45 min) followed by detoxification with activated carbon. Of all the combinations, only the treatments obtained at 135°C, 2.0% H2SO4, and 45 min and the one at 135°C, 1.25% H2SO4, and 45 min showed inhibition in the growth of the tested microorganisms. Spiked YM broths showed inhibition for the highest levels of inhibitors, either applied individually or in combination.
authors: a Department of Food Science , University of Arkansas , Fayetteville , Arkansas , USA.
authors: Deivis E
authors: DE
authors: Lujan-Rhenals
authors: Rubén O
authors: RO
authors: Morawicki
authors: Steven C
authors: SC
authors: Ricke
mesh_terms: D019342:Acetic Acid / Q000032:analysis / Q000494:pharmacology*; D000143:Acids / Q000737:chemistry; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000032:analysis / Q000494:pharmacology*; D006868:Hydrolysis; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D013025:Glycine max / Q000737:chemistry* / Q000378:metabolism; D017355:Zymomonas / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
chemical_list: D000143:Acids; D000431:Ethanol; C008046:5-hydroxymethylfurfural; D005662:Furaldehyde; D019342:Acetic Acid