title: Tissue factor expression provokes escape from tumor dormancy and leads to genomic alterations.
abstract: The coagulation system links immediate (hemostatic) and late (inflammatory, angiogenic) tissue responses to injury, a continuum that often is subverted in cancer. Here we provide evidence that tumor dormancy is influenced by tissue factor (TF), the cancer cell-associated initiator of the coagulation system and a signaling receptor. Thus, indolent human glioma cells deficient for TF remain viable but permanently dormant at the injection site for nearly a year, whereas the expression of TF leads to a step-wise transition to latent and overt tumor growth phases, a process that is preceded by recruitment of vascular (CD105(+)) and myeloid (CD11b(+) and F4/80(+)) cells. Importantly, the microenvironment orchestrated by TF expression drives permanent changes in the phenotype, gene-expression profile, DNA copy number, and DNA methylation state of the tumor cells that escape from dormancy. We postulate that procoagulant events in the tissue microenvironment (niche) may affect the fate of occult tumor cells, including their biological and genetic progression to initiate a full-blown malignancy.
authors: Montreal Children's Hospital, Research Institute of McGill University Health Centre, McGill University, Montreal, QC, Canada H3Z 2Z3.
authors: Nathalie
authors: N
authors: Magnus
authors: Delphine
authors: D
authors: Garnier
authors: Brian
authors: B
authors: Meehan
authors: Serge
authors: S
authors: McGraw
authors: Tae Hoon
authors: TH
authors: Lee
authors: Maxime
authors: M
authors: Caron
authors: Guillaume
authors: G
authors: Bourque
authors: Chloe
authors: C
authors: Milsom
authors: Nada
authors: N
authors: Jabado
authors: Jacquetta
authors: J
authors: Trasler
authors: Rafal
authors: R
authors: Pawlinski
authors: Nigel
authors: N
authors: Mackman
authors: Janusz
authors: J
authors: Rak
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D056915:DNA Copy Number Variations; D019175:DNA Methylation; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D020868:Gene Silencing; D005910:Glioma / Q000378:metabolism / Q000503:physiopathology*; D006801:Humans; D051379:Mice; D009154:Mutation / Q000235:genetics; D009385:Neoplastic Processes; D018709:Statistics, Nonparametric; D013925:Thromboplastin / Q000378:metabolism*; D059016:Tumor Microenvironment / Q000235:genetics*
chemical_list: D013925:Thromboplastin
keywords: angiogenesis; brain tumor; clotting; macrophages; oncogenes