title: MicroRNA expression profiling of cat and dog kidneys.
abstract: MicroRNAs (miRNAs) play a role in the pathogenesis of certain diseases and may serve as biomarkers. Here, we present the first analysis of miRNA expression in the kidneys of healthy cats and dogs. Kidneys were divided into renal cortex (CO) and medulla (MD), and RNA sequence analysis was performed using the mouse genome as a reference. A total of 277, 276, 295, and 297 miRNAs were detected in cat CO, cat MD, dog CO, and dog MD, respectively. By comparing the expression ratio of CO to MD, we identified highly expressed miRNAs in each tissue as follows: 41 miRNAs including miR-192-5p in cat CO; 45 miRNAs including miR-323-3p in dog CO; 78 miRNAs including miR-20a-5p in cat MD; and 11 miRNAs including miR-132-5p in dog MD. Further, the target mRNAs of these miRNAs were identified. These data provide veterinary medicine critical information regarding renal miRNA expression.
authors: Laboratory of Anatomy, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Hokkaido, Japan. Electronic address: ichi-o@vetmed.hokudai.ac.jp.
authors: Osamu
authors: O
authors: Ichii
authors: Laboratory of Anatomy, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
authors: Saori
authors: S
authors: Otsuka
authors: Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
authors: Hiroshi
authors: H
authors: Ohta
authors: Laboratory of Veterinary Clinical Pathology, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan.
authors: Akira
authors: A
authors: Yabuki
authors: Department of Endocrinology, Metabolism and Nephrology, Kochi University School of Medicine, Kochi, Japan.
authors: Taro
authors: T
authors: Horino
authors: Laboratory of Anatomy, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
authors: Yasuhiro
authors: Y
authors: Kon
mesh_terms: D000818:Animals; D002415:Cats / Q000235:genetics*; D004285:Dogs / Q000235:genetics*; D020869:Gene Expression Profiling / Q000662:veterinary*; D007668:Kidney / Q000502:physiology*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D017423:Sequence Analysis, RNA
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger
keywords: Cat; Companion animals; Dog; Kidney; MicroRNA; RNA sequencing