title: [A novel human leukocyte antigen-A*33:44 allele revealed by sequence analysis].
abstract: OBJECTIVE
  To analyze the sequence of a novel human leukocyte antigen (HLA)-A*33:44 allele.
  
  
  METHODS
  A novel HLA-A allele was found by double-stranded sequencing combined with single-stranded sequencing. The frequency of the novel allele was determined by population survey.
  
  
  RESULTS
  Genomic sequence of this novel HLA-A*33:44 allele (accession No. HQ873871) has differed from HLA-B*33:03:01 by one nucleotide in exon 4, which resulted in nt 866 G→ A substitution, which results in an amino acid substitution from Gly(GGT) to Asp(GAT) at codon 265. This alternation is a new single nucleotide polymorphism compared with other HLA-A alleles. The frequency of this new allele is less than 0.0003 in Chinese Han population.
  
  
  CONCLUSIONS
  A mutation has been found in exon 4 of the novel HLA-A*33:44 allele, which may provide more information for HLA gene study.
authors: Institute of Blood Transfusion, Guangzhou Blood Center, Guangzhou, Guangdong 510095, P.R.China. 779684055@qq.com.
authors: Haoqiang
authors: H
authors: Ding
authors: Xin
authors: X
authors: Ye
authors: Yingfeng
authors: Y
authors: Huang
authors: Yuan
authors: Y
authors: Shao
authors: Yangkai
authors: Y
authors: Chen
authors: Wenjie
authors: W
authors: Xia
authors: Baiyu
authors: B
authors: Xiong
mesh_terms: D000328:Adult; D000483:Alleles; D019943:Amino Acid Substitution; D044466:Asian People / Q000235:genetics*; D005260:Female; D015234:HLA-A Antigens / Q000235:genetics*; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D015234:HLA-A Antigens; C082945:HLA-A*33 antigen