title: Expansion of human hematopoietic stem/progenitor cells on decellularized matrix scaffolds.
abstract: Umbilical cord blood (UCB) is one of the richest sources for hematopoietic stem/progenitor cells (HSPCs), with more than 3000 transplantations performed each year for the treatment of leukemia and other bone marrow, immunological, and hereditary diseases. However, transplantation of single cord blood units is mostly restricted to children, due to the limited number of HSPC per unit. This unit develops a method to increase the number of HSPCs in laboratory conditions by using cell-free matrices from bone marrow cells that mimic 'human-body niche-like' conditions as biological scaffolds to support the ex vivo expansion of HSPCs. In this unit, we describe protocols for the isolation and characterization of HSPCs from UCB and their serum-free expansion on decellularized matrices. This method may also help to provide understanding of the biochemical organization of hematopoietic niches and lead to suggestions regarding the design of tissue engineering-based biomimetic scaffolds for HSPC expansion for clinical applications.
authors: CSIR Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India.|Geelong Technology Precinct, Deakin University, Geelong, Victoria, Australia.|Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia.
authors: Abhilasha
authors: A
authors: Tiwari
authors: Lowy Cancer Research Centre, University of New South Wales, New South Wales, Australia.
authors: Melinda L
authors: ML
authors: Tursky
authors: Geelong Technology Precinct, Deakin University, Geelong, Victoria, Australia.
authors: Mark A
authors: MA
authors: Kirkland
authors: CSIR Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India.
authors: Gopal
authors: G
authors: Pande
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002469:Cell Separation; D005109:Extracellular Matrix / Q000378:metabolism*; D005312:Fetal Blood / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D018952:Antigens, CD34; D015415:Biomarkers
keywords: decellularized biological scaffold; extracellular matrix; hematopoietic niche; stem cell expansion; umbilical cord blood