title: Perirenal fat associated with microalbuminuria in obese rats.
abstract: OBJECTIVE
  To determine whether perirenal fat is associated with increased urinary albumin excretion and whether perirenal fat affects renal vascular endothelial function in obese rats.
  
  
  METHODS
  Wistar rats were randomly divided into normal and obesity group, which were fed with normal and high-fat diet, respectively. Blood and urine samples were collected. Endothelial function of the aorta was determined by measuring endothelium-dependent vasodilatation. Renal tissues were collected for CD34 immunohistochemistry and free fatty acids (FFA) measurement. Levels of glomerular nitric oxide (NO) and reactive oxygen species (ROS) were measured.
  
  
  RESULTS
  After 24 weeks, plasma FFA, high-sensitivity C-reactive protein, and malondialdehyde levels were elevated and were significantly higher in renal venous blood than in jugular venous blood in obese rats. Urinary albumin/creatinine ratio, glomerular CD34 expression, glomerular ROS level, and renal cortex FFA levels were higher in obese rats. Endothelial dysfunction was more severe in the infra-renal aorta than in the thoracic aorta in obese rats. Plasma adiponectin and glomerular NO levels were lower in obese rats.
  
  
  CONCLUSIONS
  Perirenal fat is associated with increased urinary albumin excretion in obese rats. The mechanism may be renal vascular endothelial dysfunction caused by increased oxidative stress and activation of inflammatory molecular pathways due to elevated FFA and low adiponectin levels.
authors: Department of Endocrinology of the Affiliated Hospital, Clinical College, Weifang Medical University, No. 2428, Yuhe Road, Weifang, 261031, Shandong, China.
authors: Ningning
authors: N
authors: Hou
authors: Fang
authors: F
authors: Han
authors: Mingling
authors: M
authors: Wang
authors: Na
authors: N
authors: Huang
authors: Jiangang
authors: J
authors: Zhao
authors: Xue
authors: X
authors: Liu
authors: Xiaodong
authors: X
authors: Sun
mesh_terms: D052242:Adiponectin / Q000097:blood; D000419:Albuminuria / Q000209:etiology / Q000503:physiopathology*; D000818:Animals; D001013:Aorta, Thoracic / Q000503:physiopathology; D002097:C-Reactive Protein / Q000378:metabolism; D003404:Creatinine / Q000652:urine; D004727:Endothelium / Q000503:physiopathology*; D005227:Fatty Acids / Q000097:blood; D006984:Hypertrophy; D050152:Intra-Abdominal Fat / Q000503:physiopathology*; D007601:Jugular Veins; D007678:Kidney Glomerulus / Q000737:chemistry / Q000473:pathology*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009569:Nitric Oxide / Q000032:analysis; D009765:Obesity / Q000150:complications / Q000503:physiopathology*; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000032:analysis; D012082:Renal Veins; D014664:Vasodilation
chemical_list: D052242:Adiponectin; D005227:Fatty Acids; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D008315:Malondialdehyde; D002097:C-Reactive Protein; D003404:Creatinine