title: Sample cleanup using solid-phase dispersive extraction for determination of vancomycin in serum.
abstract: A cleanup method employing quick and simple solid-phase dispersive extraction (SPDE) was investigated for its potential use in the determination of vancomycin (VCM) in serum by liquid chromatography/mass-spectrometry (LC/MS). SPDE was observed to be more rapid than conventional cartridge-type solid-phase extraction (SPE). In addition, in the analysis of viscous samples such as serum containing many proteins, SPDE could satisfactorily remove proteins even if deproteinization was not performed beforehand. The limit of detection (S/N = 3) and the limit of quantification (S/N > 10) of VCM by LC/MS were 0.05 and 0.2 ng/mL, respectively. The average recoveries of VCM from pooled serum spiked at 2, 10, and 100 ng/mL were 90.0, 90.8, and 98.6%, respectively. The repeatabilities were 7.5, 6.8, and 2.8%, and the intermediate precision values were 8.5, 6.8, and 7.0%, respectively. This suggests that the developed analytical method combing SPDE is useful for the determination of VCM in serum.
authors: Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University.
authors: Yasuhiro
authors: Y
authors: Sakamoto
authors: Yuki
authors: Y
authors: Jinno
authors: Ikumi
authors: I
authors: Shinodzuka
authors: Yusuke
authors: Y
authors: Iwasaki
authors: Rie
authors: R
authors: Ito
authors: Koichi
authors: K
authors: Saito
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods*; D001798:Blood Proteins / Q000302:isolation & purification*; D002853:Chromatography, Liquid; D007202:Indicators and Reagents / Q000737:chemistry; D013058:Mass Spectrometry; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D013997:Time Factors; D014640:Vancomycin / Q000097:blood*
chemical_list: D001798:Blood Proteins; D007202:Indicators and Reagents; D014640:Vancomycin