title: Coffea arabica instant coffee--chemical view and immunomodulating properties.
abstract: Results of chemical analyses and immunological studies of two Coffea arabica instant coffee powders obtained by freeze-dried (ICPf) and spray-dried (ICPs) procedures, and arabinogalactan-protein (AGP3) obtained from ICPf are presented. For instant coffee powders no significant differences have been found in carbohydrate (ICPf: 37%, ICPs: 38%) as well as in caffeine (ICPf: 3.0%, ICPs: 3.4%) contents. Their (1)H NMR spectra revealed differences in trigonelline and chlorogenic acids content and in a degree of AGP backbone substitution. Immunobiological tests of all samples (ICPf, ICPs, AGP2 and AGP3) revealed a significant immunostimulatory effect on induction of interleukin 2 and free radicals secretion by mice immunocytes. Moreover, tests revealed more pronounced effect of arabinogalactans AGP2 and AGP3 compared to instant coffee powders (ICPf and ICPs).
authors: Institute of Chemistry, Department of Glycomaterials, Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovakia. Electronic address: chemcape@savba.sk.
authors: Peter
authors: P
authors: Capek
authors: Institute of Chemistry, Department of Immunochemistry of Glycoconjugates, Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovakia.
authors: Ema
authors: E
authors: Paulovičová
authors: Institute of Chemistry, Department of Analytical, Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovakia.
authors: Mária
authors: M
authors: Matulová
authors: Institute of Chemistry, Department of Glycomaterials, Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovakia.
authors: Danica
authors: D
authors: Mislovičová
authors: Illycaff s.p.a., Research & Innovation, via Flavia 110, I-34147 Trieste, Italy.
authors: Luciano
authors: L
authors: Navarini
authors: Illycaff s.p.a., Research & Innovation, via Flavia 110, I-34147 Trieste, Italy.
authors: Furio
authors: F
authors: Suggi-Liverani
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D040503:Coffea / Q000737:chemistry*; D005260:Female; D005609:Free Radicals / Q000276:immunology; D007159:Immunologic Tests; D007376:Interleukin-2 / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D005609:Free Radicals; D007376:Interleukin-2
keywords: Arabinogalactan-protein; Coffea arabica beans; FT-IR; Immunobiological activity; Instant coffee powder; NMR