title: Methylation of O6-methyl guanine methyltransferase gene promoter in meningiomas--comparison between tumor grades I, II, and III.
abstract: BACKGROUND
  Meningiomas are the second most common primary intracranial tumors after gliomas. Epigenetic biomarkers such as DNA methylation, which is found in many tumors and is thus important in tumorigenesis can help diagnose meningiomas and predict response to adjuvant chemotherapy. We investigated aberrant O6- methyl guanine methyltransferase (MGMT) methylation in meningiomas.
  
  
  METHODS
  Sixty-one patients were classified according to the WHO grading, and MGMT promoter methylation status was examined via the methylation-Specific PCR(MSP) method.
  
  
  RESULTS
  MGMT promoter methylation was found in 22.2% of grade I, 35% of grade I with atypical features, 36% of grade II, and 42.9% of grade III tumors.
  
  
  CONCLUSIONS
  There was an increase, albeit not statistically significant, in MGMT methylation with a rise in the tumor grade. Higher methylation levels were also observed in the male gender.
authors: Department of Biology, School of Basic Sciences, Science and Research Branch Islamic Azad University (IAU), Tehran, Iran E-mail : afshinmo2002@yahoo.com.
authors: Leila
authors: L
authors: Larijani
authors: Zahra
authors: Z
authors: Madjd
authors: Ali
authors: A
authors: Samadikuchaksaraei
authors: Shima
authors: S
authors: Younespour
authors: Hanieh
authors: H
authors: Zham
authors: Azadeh
authors: A
authors: Rakhshan
authors: Foruzan
authors: F
authors: Mohammadi
authors: Ali
authors: A
authors: Rahbari
authors: Afshin
authors: A
authors: Moradi
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D019175:DNA Methylation; D015254:DNA Modification Methylases / Q000235:genetics*; D045643:DNA Repair Enzymes / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008577:Meningeal Neoplasms / Q000235:genetics* / Q000473:pathology*; D008579:Meningioma / Q000235:genetics* / Q000473:pathology*; D008875:Middle Aged; D060787:Neoplasm Grading; D011401:Promoter Regions, Genetic; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D055815:Young Adult
chemical_list: D025521:Tumor Suppressor Proteins; D015254:DNA Modification Methylases; C515491:MGMT protein, human; D045643:DNA Repair Enzymes