title: In situ synthesis of silver-nanoparticles/bacterial cellulose composites for slow-released antimicrobial wound dressing.
abstract: Bacterial cellulose has attracted increasing attention as a novel wound dressing material, but it has no antimicrobial activity, which is one of critical skin-barrier functions in wound healing. To overcome such deficiency, we developed a novel method to synthesize and impregnate silver nanoparticles on to bacterial cellulose nanofibres (AgNP-BC). Uniform spherical silver nano-particles (10-30 nm) were generated and self-assembled on the surface of BC nano-fibers, forming a stable and evenly distributed Ag nanoparticles coated BC nanofiber. Such hybrid nanostructure prevented Ag nanoparticles from dropping off BC network and thus minimized the toxicity of nanoparticles. Regardless the slow Ag(+) release, AgNP-BC still exhibited significant antibacterial activities with more than 99% reductions in Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. Moreover, AgNP-BC allowed attachment and growth of epidermal cells with no cytotoxicity emerged. The results demonstrated that AgNP-BC could reduce inflammation and promote wound healing.
authors: School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, PR China. Electronic address: wujian.ustb@gmail.com.
authors: Jian
authors: J
authors: Wu
authors: School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, PR China. Electronic address: zhengyudong@mater.ustb.edu.cn.
authors: Yudong
authors: Y
authors: Zheng
authors: Centre for Nanotechnology & Regenerative Medicine, Division of Surgery & Interventional Science, University College London, London NW3 2QG, UK. Electronic address: wh_song@hotmail.com.
authors: Wenhui
authors: W
authors: Song
authors: School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, PR China. Electronic address: luanjiabin@gmail.com.
authors: Jiabin
authors: J
authors: Luan
authors: School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, PR China. Electronic address: yujilingyujiuyou@126.com.
authors: Xiaoxiao
authors: X
authors: Wen
authors: Hospital Affiliated to Chinese PLA General Hospital, Beijing 100853, PR China. Electronic address: wzhigu@yahoo.com.cn.
authors: Zhigu
authors: Z
authors: Wu
authors: State Key Laboratory of Advanced Metals and Materials, University of Science and Technology Beijing, Beijing 100083, PR China. Electronic address: chenxh@skl.ustb.edu.cn.
authors: Xiaohua
authors: X
authors: Chen
authors: School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, PR China. Electronic address: 15201461928@163.com.
authors: Qi
authors: Q
authors: Wang
authors: School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, PR China. Electronic address: ustbgsl@163.com.
authors: Shaolin
authors: S
authors: Guo
mesh_terms: D000890:Anti-Infective Agents / Q000008:administration & dosage* / Q000737:chemistry; D001458:Bandages; D002482:Cellulose / Q000737:chemistry*; D003692:Delayed-Action Preparations; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D012834:Silver / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D000890:Anti-Infective Agents; D003692:Delayed-Action Preparations; D012834:Silver; D002482:Cellulose
keywords: Antimicrobial activities; Bacterial cellulose; Biocompatibility; Epidermal cells; Silver nanoparticles