title: A cell-free approach to accelerate the study of protein-protein interactions in vitro.
abstract: Protein-protein interactions are highly desirable targets in drug discovery, yet only a fraction of drugs act as binding inhibitors. Here, we review the different technologies used to find and validate protein-protein interactions. We then discuss how the novel combination of cell-free protein expression, AlphaScreen and single-molecule fluorescence spectroscopy can be used to rapidly map protein interaction networks, determine the architecture of protein complexes, and find new targets for drug discovery.
authors: Institute for Molecular Bioscience , University of Queensland , Saint Lucia, Queensland , Australia.
authors: E
authors: E
authors: Sierecki
authors: Institute for Molecular Bioscience , University of Queensland , Saint Lucia, Queensland , Australia.
authors: N
authors: N
authors: Giles
authors: Institute for Molecular Bioscience , University of Queensland , Saint Lucia, Queensland , Australia.
authors: M
authors: M
authors: Polinkovsky
authors: Institute for Molecular Bioscience , University of Queensland , Saint Lucia, Queensland , Australia.
authors: M
authors: M
authors: Moustaqil
authors: Institute for Molecular Bioscience , University of Queensland , Saint Lucia, Queensland , Australia.
authors: K
authors: K
authors: Alexandrov
authors: Institute for Molecular Bioscience , University of Queensland , Saint Lucia, Queensland , Australia.
authors: Y
authors: Y
authors: Gambin
keywords: AlphaScreen; drug discovery; in vitro protein expression; protein–protein interactions; single-molecule fluorescence