title: Biofilm-degrading enzymes from Lysobacter gummosus.
abstract: Biofilm-degrading enzymes could be used for the gentle cleaning of industrial and medical devices and the manufacture of biofilm-resistant materials. We therefore investigated 20 species and strains of the bacterial genus Lysobacter for their ability to degrade experimental biofilms formed by Staphylococcus epidermidis, a common nosocomial pathogen typically associated with device-related infections. The highest biofilm-degradation activity was achieved by L. gummosus. The corresponding enzymes were identified by sequencing the L. gummosus genome. Partial purification of the biofilm-degrading activity from an extract of extracellular material followed by peptide mass fingerprinting resulted in the identification of two peptidases (α-lytic protease and β-lytic metalloendopeptidase) that were predicted to degrade bacterial cell walls. In addition, we identified two isoforms of a lysyl endopeptidase and an enzyme similar to metalloproteases from Vibrio spp. Potential peptidoglycan-binding C-terminal fragments of two OmpA-like proteins also co-purified with the biofilm-degrading activity. The L. gummosus genome was found to encode five isoenzymes of α-lytic protease and three isoenzymes of lysyl endopeptidase. These results indicated that the extracellular digestion of biofilms by L. gummosus depends on multiple bacteriolytic and proteolytic enzymes, which could now be exploited for biofilm control.
authors: Fraunhofer Institute for Molecular Biology and Applied Ecology IME; Project Group Bioresources; Gießen, Germany.
authors: Anke
authors: A
authors: Gökçen
authors: Fraunhofer Institute for Molecular Biology and Applied Ecology IME; Project Group Bioresources; Gießen, Germany; Justus-Liebig University of Gießen; Institute of Phytopathology and Applied Zoology at the Interdisciplinary Research Center; Gießen, Germany.
authors: Andreas
authors: A
authors: Vilcinskas
authors: Fraunhofer Institute for Molecular Biology and Applied Ecology IME; Project Group Bioresources; Gießen, Germany.
authors: Jochen
authors: J
authors: Wiesner
mesh_terms: D000898:Antibiosis; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D053528:Lysobacter / Q000201:enzymology*; D013058:Mass Spectrometry; D010447:Peptide Hydrolases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology*
chemical_list: D010447:Peptide Hydrolases
keywords: Lysobacter gummosus; Staphylococcus epidermidis; biofilm; device-related infections; lytic peptidases