title: Rapid extraction of PCR-competent DNA from recalcitrant environmental samples.
abstract: Advances in sequencing technologies have made the investigation of microbial ecology and community dynamics more tractable. The critical first step in such analyses is the efficient and representative recovery of PCR-competent DNA from complex environmental samples. All extraction protocols contain inherent biases, meaning that choice of method involves compromise between various factors, including efficiency, yield, universality, and representative extraction. Here, details are given for a routine method used in our laboratory to extract DNA from soils, sediments, biofilms, roots, and fungi.
authors: Department of Biological Sciences, Macquarie University, Sydney, NSW, Australia.
authors: Michael R
authors: MR
authors: Gillings
mesh_terms: D004783:Environmental Microbiology; D058889:Molecular Typing / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods