title: Homogenous HIV-1 subtype B quasispecies in Brazilian men and women recently infected via heterosexual transmission.
abstract: HIV has extraordinary genetic mutability, both among individuals and at the population level. However, studies of primary HIV-1 infection and serum-converters indicate that the viral population is homogeneous at the sequence level, which suggests clonal HIV transmission. It remains unclear whether this feature applies to the female population. Ten single genome amplification sequences were generated from ten individuals (five females) with recent heterosexually acquired HIV infection as determined by the serologic testing algorithm for recent HIV seroconversion. Intra-individual genetic diversity was equally low in both genders (<2 %), with mean and median variations of 0.8 and 0 %, respectively. All of the subjects were infected with clade B. Three subjects (two females) appeared to be infected by two related viral populations, and four subjects harbored non-R5 strains. Our results support the hypothesis of clonal selection for sexual transmission of HIV-1 in both genders. Future studies that generate a larger number of clones, preferably by next generation deep sequencing, are needed to confirm these results.
authors: Paulista School of Medicine, Federal University of Sao Paulo, São Paulo, SP, Brazil.
authors: Nancy Lima
authors: NL
authors: Gouveia
authors: Michelle
authors: M
authors: Camargo
authors: Marcos Montani
authors: MM
authors: Caseiro
authors: Luiz Mario Ramos
authors: LM
authors: Janini
authors: Maria Cecilia Araripe
authors: MC
authors: Sucupira
authors: Ricardo Sobhie
authors: RS
authors: Diaz
mesh_terms: D000328:Adult; D001938:Brazil / Q000453:epidemiology; D005260:Female; D014644:Genetic Variation; D015658:HIV Infections / Q000453:epidemiology / Q000523:psychology / Q000635:transmission* / Q000821:virology*; D015497:HIV-1 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012725:Sexual Behavior; D055815:Young Adult