title: Mapping free-standing homing endonuclease promoters using 5'RLM-RACE.
abstract: 5'RLM-RACE is a PCR-based technique used to map the 5' termini of transcripts in both eukaryotic and prokaryotic organisms. Free-standing homing endonuclease promoters often lack recognizable promoters making predicting the transcriptional start site challenging. Furthermore, homing endonucleases are often expressed at very low levels making transcript mapping a challenge. Here, I present a 5'RLM-RACE protocol with special considerations for the expected abundance of homing endonucleases and for their potential to be subjected to RNA processing events.
authors: Genome Sciences Centre, BC Cancer Agency, Vancouver, BC, Canada.
authors: Ewan A
authors: EA
authors: Gibb
mesh_terms: D004720:Endonucleases / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D004720:Endonucleases