title: Two calcium-dependent protein kinases from Chlamydomonas reinhardtii are transcriptionally regulated by nutrient starvation.
abstract: We report here, the transcriptional regulation of 2 Calcium Dependent Protein Kinases in response to nutrient starvation of Chlamydomonas reinhardtii vegetative cells. The CDPK proteins, CDPK1 and CDPK3; share 53% identity among themselves, a maximum of 57% and 52% to higher plants respectively and 42% to apicomplexan protozoans. We expressed a CDPK1-GFP fusion protein in the C. reinhardtii vegetative cells and showed its distribution both in the cell body and the membrane-matrix fraction of the flagella. The fusion protein exhibits mobility shift in the presence of Ca (2+), confirming its Ca (2+)-binding properties. To the best of our knowledge, this is the first report of transcriptional regulation of CDPKs from a unicellular chlorophyte in response to nutrient starvation namely acetate (A), phosphorus (P), and nitrogen (N).
authors: UM-DAE Centre for Excellence in Basic Sciences; Kalina Campus; Santacruz (E); Mumbai-400098, India.
authors: Mustafa J
authors: MJ
authors: Motiwalla
authors: UM-DAE Centre for Excellence in Basic Sciences; Kalina Campus; Santacruz (E); Mumbai-400098, India.
authors: Marilyn P
authors: MP
authors: Sequeira
authors: UM-DAE Centre for Excellence in Basic Sciences; Kalina Campus; Santacruz (E); Mumbai-400098, India.
authors: Jacinta S
authors: JS
authors: D'Souza
mesh_terms: D016825:Chlamydomonas reinhardtii / Q000201:enzymology* / Q000235:genetics; D046508:Culture Techniques; D005786:Gene Expression Regulation; D011494:Protein Kinases / Q000378:metabolism*; D017871:Calcium-Calmodulin-Dependent Protein Kinases
chemical_list: D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases
keywords: CDPK1; CDPK1-GFP; CDPK3; Chlamydomonas reinhardtii; Localization; Nutrient starvation; RT-PCR