title: Syntheses of lactosyl ceramide analogues carrying novel bifunctional BODIPY dyes directed towards the differential analysis of multiplexed glycosphingolipids by MS/MS using iTRAQ.
abstract: Lactosyl ceramide analogues carrying novel bifunctional BODIPY-based fluorescent tags were designed and synthesised for live cell imaging. Addition of azide functionality on the fluorophore facilitated isobaric tagging for quantitative multiplexed analysis of biomolecules based on tandem mass spectrometry.
authors: Japan Science and Technology Agency (JST), ERATO, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
authors: Sang-Hyun
authors: SH
authors: Son
authors: Shusaku
authors: S
authors: Daikoku
authors: Atsuko
authors: A
authors: Ohtake
authors: Katsuhiko
authors: K
authors: Suzuki
authors: Kazuya
authors: K
authors: Kabayama
authors: Yukishige
authors: Y
authors: Ito
authors: Osamu
authors: O
authors: Kanie
mesh_terms: D000818:Animals; D001896:Boron Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D002460:Cell Line; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006028:Glycosphingolipids / Q000032:analysis*; D007790:Lactosylceramides / Q000138:chemical synthesis / Q000737:chemistry*; D061848:Optical Imaging / Q000379:methods; D051381:Rats; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D006028:Glycosphingolipids; D007790:Lactosylceramides