title: A sensitive colorimetric high-throughput screening method for lipase synthetic activity assay.
abstract: A sensitive and practical high-throughput screening method for assaying lipase synthetic activity is described. Lipase-catalyzed transesterification between vinyl acetate and n-butanol in n-hexane was chosen as a model reaction. The released acetaldehyde was determined by the colorimetric method using 3-methyl-2-benzothialinone (MBTH) derivatization. In comparison with other methods, the major advantages of this process include high sensitivity, simple detection, inexpensive reagents, and low requirements for instruments.
authors: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, People's Republic of China.
authors: Jianyong
authors: J
authors: Zheng
authors: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, People's Republic of China.
authors: Xianfeng
authors: X
authors: Fu
authors: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, People's Republic of China.
authors: Xiangxian
authors: X
authors: Ying
authors: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, People's Republic of China.
authors: Yinjun
authors: Y
authors: Zhang
authors: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, People's Republic of China. Electronic address: hzwangzhao@163.com.
authors: Zhao
authors: Z
authors: Wang
mesh_terms: D000440:Butanols / Q000378:metabolism; D003124:Colorimetry / Q000379:methods*; D057075:Enzyme Assays / Q000379:methods*; D057166:High-Throughput Screening Assays; D008049:Lipase / Q000378:metabolism*; D014753:Vinyl Compounds / Q000378:metabolism
chemical_list: D000440:Butanols; D014753:Vinyl Compounds; D008049:Lipase; C011566:vinyl acetate
keywords: Derivatization; High-throughput screening; Lipase; MBTH; Synthetic activity