title: Induction of retinal progenitors and neurons from mammalian Müller glia under defined conditions.
abstract: Vision impairment caused by loss of retinal neurons affects millions of people worldwide, and currently, there is no effective treatment. Müller glia of mammalian retina may represent an under-recognized and potential source for regeneration of a wide range of retinal cell types, including retinal ganglion cells and photoreceptors. Here, we demonstrated that mouse Müller glia cells have the capacity to be reprogrammed into the retinal neuronal cell fate and are competent to give rise to photoreceptors under a defined culture condition. Inactivation of p53 released proliferation restriction of Müller glia and significantly enhanced the induction of retinal progenitor from Müller glia in culture. Moreover, following the ocular transplantation, the Müller glia-derived progenitors were differentiated toward the fates of photoreceptors and retinal ganglion cells. Together, these results demonstrate the feasibility of using Müller glia as a potential source for retinal repair and regeneration.
authors: Department of Ophthalmology and Institute for Genomic Medicine, University of California, San Diego, La Jolla, California 92093. Electronic address: j3zhao@ucsd.edu.
authors: Jack Jiagang
authors: JJ
authors: Zhao
authors: Department of Ophthalmology and Institute for Genomic Medicine, University of California, San Diego, La Jolla, California 92093.
authors: Hong
authors: H
authors: Ouyang
authors: Department of Ophthalmology and Institute for Genomic Medicine, University of California, San Diego, La Jolla, California 92093; Department of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha, Hunan 41001, China.
authors: Jing
authors: J
authors: Luo
authors: Department of Ophthalmology and Institute for Genomic Medicine, University of California, San Diego, La Jolla, California 92093.
authors: Sherrina
authors: S
authors: Patel
authors: Department of Cellular and Molecular Medicine and Institute for Genomic Medicine, University of California, San Diego, La Jolla, California 92093.
authors: Yuanchao
authors: Y
authors: Xue
authors: Department of Ophthalmology and Institute for Genomic Medicine, University of California, San Diego, La Jolla, California 92093.
authors: John
authors: J
authors: Quach
authors: Department of Ophthalmology and Institute for Genomic Medicine, University of California, San Diego, La Jolla, California 92093.
authors: Nicole
authors: N
authors: Sfeir
authors: Molecular Medicine Research Center, West China Hospital, Chengdu, Sichuan 610041, China.
authors: Meixia
authors: M
authors: Zhang
authors: Department of Cellular and Molecular Medicine and Institute for Genomic Medicine, University of California, San Diego, La Jolla, California 92093.
authors: Xiangdong
authors: X
authors: Fu
authors: Gladstone Institutes, University of California, San Francisco, San Francisco, California 94158-2261.
authors: Sheng
authors: S
authors: Ding
authors: Department of NanoEngineering, University of California, San Diego, La Jolla, California 92093.
authors: Shaochen
authors: S
authors: Chen
authors: Department of Ophthalmology and Institute for Genomic Medicine, University of California, San Diego, La Jolla, California 92093; Veterans Administration Healthcare System, San Diego, California 92161. Electronic address: kang.zhang@gmail.com.
authors: Kang
authors: K
authors: Zhang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D017931:DNA Primers; D051379:Mice; D009457:Neuroglia / Q000166:cytology*; D009474:Neurons / Q000166:cytology*; D020419:Photoreceptor Cells, Vertebrate / Q000166:cytology; D060888:Real-Time Polymerase Chain Reaction; D012160:Retina / Q000166:cytology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology*
chemical_list: D017931:DNA Primers
keywords: Muller Glia; Muller Glia, Progenitor; Photoreceptors; Proliferation; Regeneration; Reprogramming; Retina; p53