title: 3,5,7,3',4'-pentamethoxyflavone, a quercetin derivative protects DNA from oxidative challenges: potential mechanism of action.
abstract: DNA protection is one of the most important strategies in cancer therapy. Since quercetin and its derivatives are found to be potent antioxidant agents, they are able to scavenge radicals significantly. Therefore, we focused on the DNA protection activity of 3,5,7,3',4'-pentamethoxyflavone (PMF), a quercetin derivative isolated from Kaemperia parviflora. Although, PMF was found to be a very poor antioxidant compound, still it could remarkably protect DNA from oxidative damage. DNA binding assay showed that PMF bound to the minor groove of DNA, which suggests a possible mechanism for its DNA protective effects. Cellular toxicity assay on RAW 264.7 macrophages showed this compound is very safe for therapeutic applications.
authors: Department of Biotechnology, Daegu University, Kyongsan, Kyongbok 712-714, Republic of Korea.
authors: Rekha
authors: R
authors: Jakhar
authors: Department of Biotechnology, Daegu University, Kyongsan, Kyongbok 712-714, Republic of Korea.
authors: Souren
authors: S
authors: Paul
authors: Metalloenzyme Research Group and School of Biological Sciences, Chung-Ang University, Anseong 456-756, Republic of Korea.
authors: Young Rong
authors: YR
authors: Park
authors: Metalloenzyme Research Group and School of Biological Sciences, Chung-Ang University, Anseong 456-756, Republic of Korea.
authors: Jaehong
authors: J
authors: Han
authors: Department of Biotechnology, Daegu University, Kyongsan, Kyongbok 712-714, Republic of Korea. Electronic address: sckang@daegu.ac.kr.
authors: Sun Chul
authors: SC
authors: Kang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D020552:Comet Assay; D004247:DNA / Q000378:metabolism*; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D047309:Flavones / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D010851:Picrates / Q000737:chemistry; D020011:Protective Agents / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet; D013451:Sulfonic Acids / Q000737:chemistry; D018675:Toxicity Tests
chemical_list: C576901:3,5,7,3',4'-pentamethoxyflavone; D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D047309:Flavones; D016166:Free Radical Scavengers; D010851:Picrates; D020011:Protective Agents; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D009569:Nitric Oxide; D004247:DNA; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Antioxidant; Comet assay; DNA binding; DNA damage; ROS