title: Investigation of early cell-surface interactions of human mesenchymal stem cells on nanopatterned β-type titanium-niobium alloy surfaces.
abstract: Multi-potent adult mesenchymal stem cells (MSCs) derived from bone marrow have therapeutic potential for bone diseases and regenerative medicine. However, an intrinsic heterogeneity in their phenotype, which in turn results in various differentiation potentials, makes it difficult to predict the response of these cells. The aim of this study is to investigate initial cell-surface interactions of human MSCs on modified titanium alloys. Gold nanoparticles deposited on β-type Ti-40Nb alloys by block copolymer micelle nanolithography served as nanotopographical cues as well as specific binding sites for the immobilization of thiolated peptides present in several extracellular matrix proteins. MSC heterogeneity persists on polished and nanopatterned Ti-40Nb samples. However, cell heterogeneity and donor variability decreased upon functionalization of the gold nanoparticles with cyclic RGD peptides. In particular, the number of large cells significantly decreased after 24 h owing to the arrangement of cell anchorage sites, rather than peptide specificity. However, the size and number of integrin-mediated adhesion clusters increased in the presence of the integrin-binding peptide (cRGDfK) compared with the control peptide (cRADfK). These results suggest that the use of integrin ligands in defined patterns could improve MSC-material interactions, not only by regulating cell adhesion locally, but also by reducing population heterogeneity.
authors: Institute for Physical Chemistry , Ruprecht-Karl-University of Heidelberg , Heidelberg 69120 , Germany ; Max Planck Institute for Intelligent Systems , Stuttgart 70569 , Germany.
authors: Rebecca
authors: R
authors: Medda
authors: Institute for Complex Materials , IFW Dresden , PO Box 270116, Dresden 01171 , Germany.
authors: Arne
authors: A
authors: Helth
authors: Institute for Metallic Materials , IFW Dresden , PO Box 270116, Dresden 01171 , Germany.
authors: Patrick
authors: P
authors: Herre
authors: Institute for Metallic Materials , IFW Dresden , PO Box 270116, Dresden 01171 , Germany.
authors: Darius
authors: D
authors: Pohl
authors: Institute for Metallic Materials , IFW Dresden , PO Box 270116, Dresden 01171 , Germany.
authors: Bernd
authors: B
authors: Rellinghaus
authors: Institute for Physical Chemistry , Ruprecht-Karl-University of Heidelberg , Heidelberg 69120 , Germany ; Max Planck Institute for Intelligent Systems , Stuttgart 70569 , Germany.
authors: Nadine
authors: N
authors: Perschmann
authors: Institute for Advanced Study (IAS) and Center of Integrated Protein Science (CIPSM) , Technische Universität München , Lichtenbergstrasse 4, Garching 85747 , Germany.
authors: Stefanie
authors: S
authors: Neubauer
authors: Institute for Advanced Study (IAS) and Center of Integrated Protein Science (CIPSM) , Technische Universität München , Lichtenbergstrasse 4, Garching 85747 , Germany.
authors: Horst
authors: H
authors: Kessler
authors: Institute for Complex Materials , IFW Dresden , PO Box 270116, Dresden 01171 , Germany.
authors: Steffen
authors: S
authors: Oswald
authors: Institute for Complex Materials , IFW Dresden , PO Box 270116, Dresden 01171 , Germany.
authors: Jürgen
authors: J
authors: Eckert
authors: Institute for Physical Chemistry , Ruprecht-Karl-University of Heidelberg , Heidelberg 69120 , Germany ; Max Planck Institute for Intelligent Systems , Stuttgart 70569 , Germany.
authors: Joachim P
authors: JP
authors: Spatz
authors: Institute for Complex Materials , IFW Dresden , PO Box 270116, Dresden 01171 , Germany.
authors: Annett
authors: A
authors: Gebert
authors: Institute for Physical Chemistry , Ruprecht-Karl-University of Heidelberg , Heidelberg 69120 , Germany ; Max Planck Institute for Intelligent Systems , Stuttgart 70569 , Germany.
authors: Elisabetta A
authors: EA
authors: Cavalcanti-Adam
keywords: block copolymer micelle nanolithography; cell adhesion; human mesenchymal stem cells; nanopattern; titanium alloys