title: Short-fragment DNA-mediated in vivo DNA electroporation delivery.
abstract: Electroporation is an effective physical delivery method. A variety of factors have been shown to affect the electroporation-mediated gene delivery efficiency. Here we report the usefulness of noncoding short-fragment DNA (sf-DNA) for facilitating electroporation-mediated gene transfer. The plasmid pGL3-control encoding firefly luciferase was injected into tissue together with or without sf-DNA in different length or dose. Immediately after injection, the tissues were electroporated and the level of luciferase activity was assessed 24 h later. The results showed that plasmid DNA formulated with sf-DNA resulted in significant improvement in electroporation-mediated gene transfer efficiency. The effect is dose and length dependent, and also found in low-voltage electroporation. These results indicated that sf-DNA can be used as a helper molecule to improve the electroporation-mediated gene transfection efficiency.
authors: School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
authors: Jinliang
authors: J
authors: Peng
authors: Yonggang
authors: Y
authors: Zhao
authors: Yuhong
authors: Y
authors: Xu
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D004553:Electric Conductivity; D018274:Electroporation / Q000379:methods*; D005260:Female; D007267:Injections; D008156:Luciferases / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D009132:Muscles / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D014162:Transfection / Q000379:methods*
chemical_list: D004247:DNA; D008156:Luciferases