title: New antifouling platform characterized by single-molecule imaging.
abstract: Antifouling surfaces have been widely studied for their importance in medical devices and industry. Antifouling surfaces mostly achieved by methoxy-poly(ethylene glycol) (mPEG) have shown biomolecular adsorption less than 1 ng/cm(2) which was measured by surface analytical tools such as surface plasmon resonance (SPR) spectroscopy, quartz crystal microbalance (QCM), or optical waveguide lightmode (OWL) spectroscopy. Herein, we utilize a single-molecule imaging technique (i.e., an ultimate resolution) to study antifouling properties of functionalized surfaces. We found that about 600 immunoglobulin G (IgG) molecules are adsorbed. This result corresponds to ∼5 pg/cm(2) adsorption, which is far below amount for the detection limit of the conventional tools. Furthermore, we developed a new antifouling platform that exhibits improved antifouling performance that shows only 78 IgG molecules adsorbed (∼0.5 pg/cm(2)). The antifouling platform consists of forming 1 nm TiO2 thin layer, on which peptidomimetic antifouling polymer (PMAP) is robustly anchored. The unprecedented antifouling performance can potentially revolutionize a variety of research fields such as single-molecule imaging, medical devices, biosensors, and others.
authors: Department of Chemistry, ‡National Creative Research Initiative Center for Single-Molecule Systems Biology, and Department of Physics, KAIST, 291 University Rd., Daejeon 305-701, Republic of Korea.
authors: Ji Young
authors: JY
authors: Ryu
authors: In Taek
authors: IT
authors: Song
authors: K H Aaron
authors: KH
authors: Lau
authors: Phillip B
authors: PB
authors: Messersmith
authors: Tae-Young
authors: TY
authors: Yoon
authors: Haeshin
authors: H
authors: Lee
mesh_terms: D000327:Adsorption; D007074:Immunoglobulin G / Q000737:chemistry*; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods; D036103:Nanotechnology / Q000295:instrumentation*; D011108:Polymers / Q000737:chemistry*
chemical_list: D007074:Immunoglobulin G; D011108:Polymers