title: Increased 2,3-butanediol production by changing codon usages in Escherichia coli.
abstract: The natural microorganism Escherichia coli without modification is not suitable for the efficient production of 2,3-butanediol (2,3-BD) on an industrial scale because of its poor metabolic performance. Metabolic capacities of E. coli have been improved to produce 2,3-BD efficiently, the performance of which is possible for producing such a product. Codon optimization with the ribosome-binding site for the efficient production of target genes (budA and budC) was achieved by molecular engineering, which allowed the metabolic engineering to proceed to the next level. As a result, comparing the productivity in 26 H, where the amount of p18COR was 1.04 g/L and that of p18WTR was 0.41 g/L, represents an approximate 60.6% increase in the productivity of the p18WTR with codon optimization. In other words, p18COR was 2.54-fold greater than p18WTR in the production of 2,3-BD.
authors: Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, Korea; Department of Chemical Engineering, Hongik University, Seoul, Korea.
authors: Seo-Young
authors: SY
authors: Park
authors: Borim
authors: B
authors: Kim
authors: Soojin
authors: S
authors: Lee
authors: Minkyu
authors: M
authors: Oh
authors: Jong-In
authors: JI
authors: Won
authors: Jinwon
authors: J
authors: Lee
mesh_terms: D002072:Butylene Glycols / Q000032:analysis / Q000378:metabolism*; D003001:Cloning, Molecular / Q000379:methods*; D003062:Codon / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods*
chemical_list: D002072:Butylene Glycols; D003062:Codon; C026978:2,3-butylene glycol
keywords: 2,3-butanediol; Escherichia coli; acetoin reductase; acetolactate decarboxylase; codon optimization; ribosome-binding site