title: The differential interferon responses of two strains of Stat1-deficient mice do not alter susceptibility to HSV-1 and VSV in vivo.
abstract: Stat1 is a pivotal transcription factor for generation of the interferon (IFN)-dependent antiviral response. Two Stat1 knockout mouse lines have been previously generated, one deleted the N-terminal domain (ΔNTD) and one in the DNA-binding domain (ΔDBD). These widely-used strains are assumed interchangeable, and both are highly susceptible to various pathogens. In this study, primary cells derived from ΔNTD mice were shown to be significantly more responsive to IFN, and established an antiviral state with greater efficiency than cells derived from ΔDBD mice, following infection with vesicular stomatitis virus and herpes simplex virus type-1. Also, while mice from both strains succumbed rapidly and equally to virus infection, ΔDBD mice supported significantly higher replication in brains and livers than ΔNTD mice. Endpoint-type experimental comparisons of these mouse strains are therefore misleading in failing to indicate important differences in virus replication and innate response.
authors: Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Lebanon, NH 03756, United States.
authors: Sarah
authors: S
authors: Katzenell
authors: Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Lebanon, NH 03756, United States.
authors: Yufei
authors: Y
authors: Chen
authors: Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Lebanon, NH 03756, United States.
authors: Zachary M
authors: ZM
authors: Parker
authors: Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Lebanon, NH 03756, United States. Electronic address: david.a.leib@dartmouth.edu.
authors: David A
authors: DA
authors: Leib
mesh_terms: D000818:Animals; D004198:Disease Susceptibility; D006561:Herpes Simplex / Q000235:genetics / Q000276:immunology* / Q000821:virology; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000502:physiology*; D007372:Interferons; D051379:Mice; D018345:Mice, Knockout; D017434:Protein Structure, Tertiary; D050794:STAT1 Transcription Factor / Q000737:chemistry / Q000172:deficiency / Q000235:genetics / Q000276:immunology*; D054243:Vesicular Stomatitis / Q000235:genetics / Q000276:immunology* / Q000821:virology; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics / Q000502:physiology*; D014779:Virus Replication
chemical_list: D050794:STAT1 Transcription Factor; C494080:Stat1 protein, mouse; D007372:Interferons
keywords: Herpes simplex virus; Innate immunity; Stat1; Vesicular stomatitis virus