title: [Effect of yangyin yiqi huoxue recipe on immune balance of Th1/Th2 in serum and submaxillary glands of NOD mice with Sjogren's syndrome].
abstract: OBJECTIVE
  To observe the effect of Yangyin Yiqi Huoxue Recipe (YYHR) on expression of interferon-gamma (IFN-gamma), IL-2, IL-4, IL-10, and immune balance of Th1/Th2 in serum and submaxillary glands of non-obese diabetic (NOD) mice with Sjogren's syndrome (SS), and to explore its mechanisms.
  
  
  METHODS
  Totally 32 NOD mice were randomly into 4 groups, i.e., the model group, the Chinese medicine group [CM, administered with YYHR at the dose of 0.4 mL by gavage (100 g/kg)], the Western medicine group [WM group, administered with hydroxychloroquine 0.4 mL by gavage (60 mg/kg)], and the combined group [administered with YYHR (50 g/kg) and hydroxychloroquine (60 mg/kg) 0.4 mL by gavage], 8 mice in each group. Eight Balb/C mice were recruited as the normal control group. Mice were sacrificed to withdraw blood after 8 weeks. The submaxillary gland was excised. Serum levels of IFN-gamma, IL-2, IL-4, and IL-10 were detected by ELISA. Protein expression of IFN-gamma, IL-2, IL-4, and IL-10 in submaxillary glands was detected by SP method.
  
  
  RESULTS
  Compared with the normal group, levels of IFN-gamma, IL-2, IL-4, and IL-10 in serum and submaxillary glands all increased in the model group (P < 0.05). Levels of IFN-gamma and IL-10 in serum in the CM group and the combined group were lower than those of the WM group (P < 0.05). Serum levels of IFN-gamma and IL-2 in submaxillary glands in combined group were lower than those of the WM group (P < 0.05). The ratio of IFN-gamma/IL-4 in serum and submaxillary glands in the model group were higher than that of the rest groups (P < 0.05). Besides, it was the nearest to that of the normal group.
  
  
  CONCLUSIONS
  YYHR could decrease the levels of Th1 and Th2 related cytokines and the ratio of IFN-gamma/IL-4 in serum and submaxillary glands of NOD mice with SS. It could achieve therapeutic effects through adjusting immune balance of Th1/Th2 in SS mice.
authors: Department of Chinese Medicine, First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou 310003, China.
authors: Guo-Lin
authors: GL
authors: Wu
authors: Department of Internal Medicine, Qujing City Hospital of Traditional Chinese Medicine, Qujing 655000, China.
authors: Xing-Hong
authors: XH
authors: Pu
authors: College of Basic Medicine, Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors: Tian-Yi
authors: TY
authors: Li
authors: Department of Chinese Medicine, First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou 310003, China.
authors: Guo-You
authors: GY
authors: Yu
authors: Department of Chinese Medicine, First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou 310003, China.
authors: Wen-Wen
authors: WW
authors: Lu
authors: College of Basic Medicine, Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors: Yong-Sheng
authors: YS
authors: Fan
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D007371:Interferon-gamma / Q000097:blood; D007378:Interleukins / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D016688:Mice, Inbred NOD; D044967:Serum / Q000276:immunology*; D012859:Sjogren's Syndrome / Q000097:blood* / Q000188:drug therapy / Q000276:immunology*; D013363:Submandibular Gland / Q000276:immunology*; D058408:Th1-Th2 Balance
chemical_list: D004365:Drugs, Chinese Herbal; D007378:Interleukins; D007371:Interferon-gamma; C033946:yiki-huoxue