title: Dendritic cells coordinate innate immunity via MyD88 signaling to control Listeria monocytogenes infection.
abstract: Listeria monocytogenes (LM), a facultative intracellular Gram-positive pathogen, can cause life-threatening infections in humans. In mice, the signaling cascade downstream of the myeloid differentiation factor 88 (MyD88) is essential for proper innate immune activation against LM, as MyD88-deficient mice succumb early to infection. Here, we show that MyD88 signaling in dendritic cells (DCs) is sufficient to mediate the protective innate response, including the production of proinflammatory cytokines, neutrophil infiltration, bacterial clearance, and full protection from lethal infection. We also demonstrate that MyD88 signaling by DCs controls the infection rates of CD8α(+) cDCs and thus limits the spread of LM to the T cell areas. Furthermore, in mice expressing MyD88 in DCs, inflammatory monocytes, which are required for bacterial clearance, are activated independently of intrinsic MyD88 signaling. In conclusion, CD11c(+) conventional DCs critically integrate pathogen-derived signals via MyD88 signaling during early infection with LM in vivo.
authors: Institute for Infection Immunology, TWINCORE, Center for Experimental and Clinical Infection Research, a Joint Venture between the Medical School Hannover (MHH) and the Helmholtz Center for Infection Research (HZI), 30625 Hannover, Germany.
authors: Catharina
authors: C
authors: Arnold-Schrauf
authors: Institute for Infection Immunology, TWINCORE, Center for Experimental and Clinical Infection Research, a Joint Venture between the Medical School Hannover (MHH) and the Helmholtz Center for Infection Research (HZI), 30625 Hannover, Germany.
authors: Markus
authors: M
authors: Dudek
authors: Institute for Infection Immunology, TWINCORE, Center for Experimental and Clinical Infection Research, a Joint Venture between the Medical School Hannover (MHH) and the Helmholtz Center for Infection Research (HZI), 30625 Hannover, Germany.
authors: Anastasia
authors: A
authors: Dielmann
authors: Institut National de la Santé et de la Recherche Médicale (INSERM) U932, Institut Curie, 75005 Paris, France.
authors: Luigia
authors: L
authors: Pace
authors: Institute for Infection Immunology, TWINCORE, Center for Experimental and Clinical Infection Research, a Joint Venture between the Medical School Hannover (MHH) and the Helmholtz Center for Infection Research (HZI), 30625 Hannover, Germany.
authors: Maxine
authors: M
authors: Swallow
authors: Institute for Infection Immunology, TWINCORE, Center for Experimental and Clinical Infection Research, a Joint Venture between the Medical School Hannover (MHH) and the Helmholtz Center for Infection Research (HZI), 30625 Hannover, Germany.
authors: Friederike
authors: F
authors: Kruse
authors: Department of Medicine I for Gastroenterology, Infectious Disease and Rheumatology, Campus Benjamin Franklin, Charité-Universitätsmedizin Berlin, 12203 Berlin, Germany.
authors: Anja A
authors: AA
authors: Kühl
authors: Chirurgische Klinik und Poliklinik, Technische Universität München, 81675 Munich, Germany.
authors: Bernhard
authors: B
authors: Holzmann
authors: Institute for Infection Immunology, TWINCORE, Center for Experimental and Clinical Infection Research, a Joint Venture between the Medical School Hannover (MHH) and the Helmholtz Center for Infection Research (HZI), 30625 Hannover, Germany.
authors: Luciana
authors: L
authors: Berod
authors: Institute for Infection Immunology, TWINCORE, Center for Experimental and Clinical Infection Research, a Joint Venture between the Medical School Hannover (MHH) and the Helmholtz Center for Infection Research (HZI), 30625 Hannover, Germany. Electronic address: sparwasser.tim@mh-hannover.de.
authors: Tim
authors: T
authors: Sparwasser
mesh_terms: D000818:Animals; D039521:CD11c Antigen / Q000235:genetics / Q000378:metabolism; D016827:CD8 Antigens / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D007113:Immunity, Innate; D008088:Listeriosis / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics / Q000378:metabolism*; D009504:Neutrophils / Q000276:immunology; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D039521:CD11c Antigen; D016827:CD8 Antigens; C431698:CD8alpha antigen; D016207:Cytokines; C507780:Myd88 protein, mouse; D053594:Myeloid Differentiation Factor 88