title: Two amino acid residues in ion channel protein M2 and polymerase protein PA contribute to replication difference of H5N1 influenza viruses in mice.
abstract: A/swine/Fujian/1/2001 (FJ1) and A/Duck/Zhejiang/52/2000 (DK52) are H5N1 influenza viruses that are lethal in chickens. However, in mice, FJ1 is highly pathogenic, whereas DK52 cannot replicate at all. In this study, we used reverse genetics to demonstrate that amino acid residues at position 54 of polymerase acidic protein (PA) and position 26 of ion channel protein M2 of FJ1 and DK52 are important determinants for replication in mice. In addition, we prove that M2 and PA proteins contribute to the replication of H5N1 viruses in mice.
authors: Shandong Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, People's Republic of China.
authors: Chuantian
authors: C
authors: Xu
authors: Animal Influenza Laboratory of the Ministry of Agriculture and National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, 427 Maduan Street, Harbin 150001, People's Republic of China.
authors: Hualan
authors: H
authors: Chen
authors: Shandong Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, People's Republic of China.
authors: Beixia
authors: B
authors: Hu
authors: Shandong Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, People's Republic of China.
authors: Shaohua
authors: S
authors: Yang
authors: Shandong Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, People's Republic of China.
authors: Lin
authors: L
authors: Zhang
authors: Shandong Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, People's Republic of China.
authors: Qinghua
authors: Q
authors: Huang
authors: Shandong Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, People's Republic of China. Electronic address: xcttaian2002@163.com.
authors: Xiumei
authors: X
authors: Zhang
keywords: M2; Mice; PA; Replication