title: Anticarcinogenic activity of polyphenolic extracts from grape stems against breast, colon, renal and thyroid cancer cells.
abstract: A major part of the wineries' wastes is composed of grape stems which are discarded mainly in open fields and cause environmental problems due mainly to their high polyphenolic content. The grape stem extracts' use as a source of high added value polyphenols presents great interest because this combines a profitable venture with environmental protection close to wine-producing zones. In the present study, at first, the Total Polyphenolic Content (TPC) and the polyphenolic composition of grape stem extracts from four different Greek Vitis vinifera varieties were determined by HPLC methods. Afterwards, the grape stem extracts were examined for their ability to inhibit growth of colon (HT29), breast (MCF-7 and MDA-MB-23), renal (786-0 and Caki-1) and thyroid (K1) cancer cells. The cancer cells were exposed to the extracts for 72 h and the effects on cell growth were evaluated using the SRB assay. The results indicated that all extracts inhibited cell proliferation, with IC₅₀ values of 121-230 μg/ml (MCF-7), 121-184 μg/ml (MDA-MD-23), 175-309 μg/ml (HT29), 159-314 μg/ml (K1), 180-225 μg/ml (786-0) and 134->400 μg/ml (Caki-1). This is the first study presenting the inhibitory activity of grape stem extracts against growth of colon, breast, renal and thyroid cancer cells.
authors: Cell Culture, Molecular Modelling and Drug Design Lab., Symeonidion Research Center, Theagenion Cancer Hospital, Thessaloniki 54639, Greece.
authors: Despina
authors: D
authors: Sahpazidou
authors: Cell Culture, Molecular Modelling and Drug Design Lab., Symeonidion Research Center, Theagenion Cancer Hospital, Thessaloniki 54639, Greece.
authors: George D
authors: GD
authors: Geromichalos
authors: Department of Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26 & Aiolou, Larissa 41221, Greece.
authors: Dimitrios
authors: D
authors: Stagos
authors: Chemistry Laboratory, Agricultural University of Athens, Iera Odos 75, Athens 11855, Greece.
authors: Anna
authors: A
authors: Apostolou
authors: Chemistry Laboratory, Agricultural University of Athens, Iera Odos 75, Athens 11855, Greece.
authors: Serkos A
authors: SA
authors: Haroutounian
authors: Department of Forensic Sciences and Toxicology, Medical School, University of Crete, Heraklion 71003, Greece.
authors: Aristidis M
authors: AM
authors: Tsatsakis
authors: Laboratory of Anatomy-Histology-Embryology, Medical School, University of Crete, Heraklion 71003, Greece.
authors: George N
authors: GN
authors: Tzanakakis
authors: Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115, USA; Spherix Consulting, Inc., 6430 Rockledge Drive #503, Bethesda, MD 20817, USA.
authors: A Wallace
authors: AW
authors: Hayes
authors: Department of Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26 & Aiolou, Larissa 41221, Greece. Electronic address: dkouret@uth.gr.
authors: Dimitrios
authors: D
authors: Kouretas
mesh_terms: D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology / Q000517:prevention & control; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000473:pathology / Q000517:prevention & control; D006801:Humans; D007680:Kidney Neoplasms / Q000473:pathology / Q000517:prevention & control; D010936:Plant Extracts / Q000494:pharmacology*; D018547:Plant Stems; D059808:Polyphenols / Q000494:pharmacology*; D013964:Thyroid Neoplasms / Q000473:pathology / Q000517:prevention & control; D027843:Vitis
chemical_list: D016588:Anticarcinogenic Agents; D010936:Plant Extracts; D059808:Polyphenols
keywords: Anticancer activity; Grape stem extracts; Polyphenols; Vitis vinifera