title: Guiding intracortical brain tumour cells to an extracortical cytotoxic hydrogel using aligned polymeric nanofibres.
abstract: Glioblastoma multiforme is an aggressive, invasive brain tumour with a poor survival rate. Available treatments are ineffective and some tumours remain inoperable because of their size or location. The tumours are known to invade and migrate along white matter tracts and blood vessels. Here, we exploit this characteristic of glioblastoma multiforme by engineering aligned polycaprolactone (PCL)-based nanofibres for tumour cells to invade and, hence, guide cells away from the primary tumour site to an extracortical location. This extracortial sink is a cyclopamine drug-conjugated, collagen-based hydrogel. When aligned PCL-nanofibre films in a PCL/polyurethane carrier conduit were inserted in the vicinity of an intracortical human U87MG glioblastoma xenograft, a significant number of human glioblastoma cells migrated along the aligned nanofibre films and underwent apoptosis in the extracortical hydrogel. Tumour volume in the brain was significantly lower following insertion of aligned nanofibre implants compared with the application of smooth fibres or no implants.
authors: 1] Neurological Biomaterials and Cancer Therapeutics Laboratory, Wallace H. Coulter Department of Biomedical Engineering Georgia Institute of Technology/Emory University School of Medicine, 313 Ferst Drive, Atlanta Georgia 30332 USA [2].
authors: Anjana
authors: A
authors: Jain
authors: Neurological Biomaterials and Cancer Therapeutics Laboratory, Wallace H. Coulter Department of Biomedical Engineering Georgia Institute of Technology/Emory University School of Medicine, 313 Ferst Drive, Atlanta Georgia 30332 USA.
authors: Martha
authors: M
authors: Betancur
authors: Neurological Biomaterials and Cancer Therapeutics Laboratory, Wallace H. Coulter Department of Biomedical Engineering Georgia Institute of Technology/Emory University School of Medicine, 313 Ferst Drive, Atlanta Georgia 30332 USA.
authors: Gaurangkumar D
authors: GD
authors: Patel
authors: Neurological Biomaterials and Cancer Therapeutics Laboratory, Wallace H. Coulter Department of Biomedical Engineering Georgia Institute of Technology/Emory University School of Medicine, 313 Ferst Drive, Atlanta Georgia 30332 USA.
authors: Chandra M
authors: CM
authors: Valmikinathan
authors: Neurological Biomaterials and Cancer Therapeutics Laboratory, Wallace H. Coulter Department of Biomedical Engineering Georgia Institute of Technology/Emory University School of Medicine, 313 Ferst Drive, Atlanta Georgia 30332 USA.
authors: Vivek J
authors: VJ
authors: Mukhatyar
authors: Neurological Biomaterials and Cancer Therapeutics Laboratory, Wallace H. Coulter Department of Biomedical Engineering Georgia Institute of Technology/Emory University School of Medicine, 313 Ferst Drive, Atlanta Georgia 30332 USA.
authors: Ajit
authors: A
authors: Vakharia
authors: Neurological Biomaterials and Cancer Therapeutics Laboratory, Wallace H. Coulter Department of Biomedical Engineering Georgia Institute of Technology/Emory University School of Medicine, 313 Ferst Drive, Atlanta Georgia 30332 USA.
authors: S Balakrishna
authors: SB
authors: Pai
authors: Department of Neurosurgery Children's Health Care of Atlanta Georgia 30342 USA.
authors: Barunashish
authors: B
authors: Brahma
authors: Department of Pediatrics, Aflac Cancer And Blood Disorders Center Emory University School of Medicine Atlanta, Georgia 30322 USA.
authors: Tobey J
authors: TJ
authors: MacDonald
authors: Neurological Biomaterials and Cancer Therapeutics Laboratory, Wallace H. Coulter Department of Biomedical Engineering Georgia Institute of Technology/Emory University School of Medicine, 313 Ferst Drive, Atlanta Georgia 30332 USA.
authors: Ravi V
authors: RV
authors: Bellamkonda
mesh_terms: D001932:Brain Neoplasms / Q000473:pathology*; D005909:Glioblastoma / Q000473:pathology*; D064593:Heterografts; D006801:Humans; D020100:Hydrogels; D057139:Nanofibers; D011108:Polymers / Q000737:chemistry*
chemical_list: D020100:Hydrogels; D011108:Polymers