title: Production of monoclonal antibodies.
abstract: This unit describes the production of monoclonal antibodies beginning with immunization, cell fusion, and selection. Support protocols are provided for screening primary hybridoma supernatants for antibodies of desired specificity, establishment of stable hybridoma lines, cloning of these B cell lines by limiting dilution to obtain monoclonal lines, and preparation of cloning/expansion medium. An alternate protocol describes cell fusion and one-step selection and cloning of hybridomas utilizing a semi-solid methylcellulose-based medium (ClonaCell-HY from StemCell Technologies).
authors: Washington University School of Medicine, St. Louis, Missouri.
authors: Wayne M
authors: WM
authors: Yokoyama
authors: StemCell Technologies, Inc, Vancouver, British Columbia, Canada.
authors: Michelle
authors: M
authors: Christensen
authors: StemCell Technologies, Inc, Vancouver, British Columbia, Canada.
authors: Gary Dos
authors: GD
authors: Santos
authors: StemCell Technologies, Inc, Vancouver, British Columbia, Canada.
authors: Diane
authors: D
authors: Miller
authors: Boehringer Ingelheim Pharmaceuticals, Inc, Ridgefield, Connecticut.
authors: Jason
authors: J
authors: Ho
authors: Boehringer Ingelheim Pharmaceuticals, Inc, Ridgefield, Connecticut.
authors: Tao
authors: T
authors: Wu
authors: Boehringer Ingelheim Pharmaceuticals, Inc, Ridgefield, Connecticut.
authors: Michael
authors: M
authors: Dziegelewski
authors: Boehringer Ingelheim Pharmaceuticals, Inc, Ridgefield, Connecticut.
authors: Francisca A
authors: FA
authors: Neethling
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D001402:B-Lymphocytes / Q000276:immunology*; D018929:Cell Culture Techniques / Q000379:methods*; D002459:Cell Fusion / Q000379:methods; D002999:Clone Cells; D000939:Epitopes; D006825:Hybridomas; D007114:Immunization; D008747:Methylcellulose / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D008747:Methylcellulose
keywords: B cell hybridomas; limiting dilution; monoclonal antibodies; semi-solid methylcellulose-based medium