title: Probe development for detection of TERRA 1 intramolecular G-quadruplex formation using a fluorescent adenosine derivative.
abstract: We developed a probing system to detect the intramolecular G-quadruplex of telomeric repeat-containing RNA (TERRA 1). We used a fluorescent adenosine derivative rA(py) as a fluorophore and incorporated it into the dangling position of the parallel-type G-quadruplex sequence of TERRA 1. The rA(py)-modified G-quadruplex structure exhibited a strong fluorescence emission signal, while the emission signals of the single-strand and duplex structures were much lower.
authors: Department of Chemistry, Chonbuk National University, Jeonju 561-756, South Korea.
authors: In Sun
authors: IS
authors: Kim
authors: Department of Chemistry, Chonbuk National University, Jeonju 561-756, South Korea. Electronic address: yseo@jbnu.ac.kr.
authors: Young Jun
authors: YJ
authors: Seo
mesh_terms: D002942:Circular Dichroism; D005456:Fluorescent Dyes / Q000737:chemistry*; D054856:G-Quadruplexes; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism; D012313:RNA / Q000032:analysis* / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013696:Temperature
chemical_list: D005456:Fluorescent Dyes; D009841:Oligonucleotides; D012313:RNA
keywords: Fluorophore; G-quadruplex; Probe; RNA; TERRA 1