title: Serum microRNAs as biomarkers in patients undergoing prostate biopsy: results from a prospective multi-center study.
abstract: OBJECTIVE
  Increased levels of microRNAs in serum/plasma have been identified in various malignancies. We aimed to investigate serum levels of miR-26a-1 and miR-141 in patients undergoing prostate biopsy clinical suspicious for prostate cancer (PCA) in a prospective multi-center study.
  
  
  METHODS
  Pre-biopsy serum samples of 170 patients were collected in three different study Centres. Serum RNA was isolated, and microRNA lev-els were quantified using real-time PCR. Relative miR-26a -1 and miR-141 levels were determined using RNU1-4 and SNORD43 as reference genes.
  
  
  RESULTS
  After exclusion of pa-tients with metastatic prostate cancer (n=9) and isolation failures (n=28), 133 patients (prostate cancer n=54, non-malignant n=79) were used for further analysis. The levels of miR-26a-1 and miR-141 were similar in patients with positive and negative biopsies. We observed a significant increase of miR-141 in patients with higher Gleason Score.
  
  
  CONCLUSIONS
  The analysis of circulating microRNAs does not seem to help identify patients with cancer undergoing prostate biopsy. However, their levels may be useful to identify patients with high-risk prostate cancer.
authors: Universitätsklinikum Bonn, Klinik und Poliklinik für Urologie und Kinderurologie, Bonn, Germany; Sigmund-Freud-Straße 25, 53105 Bonn. joerg.ellinger@ukb.uni-bonn.de.
authors: Anna M
authors: AM
authors: Westermann
authors: Doris
authors: D
authors: Schmidt
authors: Stefan
authors: S
authors: Holdenrieder
authors: Rudolf
authors: R
authors: Moritz
authors: Axel
authors: A
authors: Semjonow
authors: Matthias
authors: M
authors: Schmidt
authors: Glen
authors: G
authors: Kristiansen
authors: Stefan C
authors: SC
authors: Müller
authors: Jörg
authors: J
authors: Ellinger
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D001706:Biopsy; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D008875:Middle Aged; D060787:Neoplasm Grading; D011446:Prospective Studies; D011471:Prostatic Neoplasms / Q000097:blood* / Q000235:genetics / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; C533895:MIRN141 microRNA, human; C528163:MIRN26 microRNA, human
keywords: Prostate cancer; biomarker; miR-141; miR-26a-1; microRNA; serum