title: New diterpenoid alkaloids from Aconitum coreanum and their anti-arrhythmic effects on cardiac sodium current.
abstract: Two new diterpenoid alkaloids, Guan-Fu base J (GFJ, 1) and Guan-Fu base N (GFN, 2) along with nineteen known alkaloids (3-21) were isolated from the roots of Aconitum coreanum (Lèvl.) Rapaics, which is the raw material of a new approval anti-arrhythmia drug "Acehytisine Hydrochloride". The structures of isolated compounds were established by means of 1D, 2D NMR spectroscopic and chemical methods. All isolates obtained in the present study were evaluated for their inhibitory effects on blocking the ventricular specific sodium current using a whole-cell patch voltage-clamp technique. Among these 21 compounds, Guan-Fu base S (GFS, 3) showed the strongest inhibitory effect with an IC50 value of 3.48 μM, and only hetisine-type C20 diterpenoid alkaloids showed promising IC50 values for further development.
authors: School of Chinese Medicine, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China.
authors: Bei-Ni
authors: BN
authors: Xing
authors: Research Division of Pharmacology, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China.
authors: Si-Si
authors: SS
authors: Jin
authors: School of Chinese Medicine, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China.
authors: Hao
authors: H
authors: Wang
authors: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, People's Republic of China.
authors: Qing-Fa
authors: QF
authors: Tang
authors: School of Chinese Medicine, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China.
authors: Jing-Han
authors: JH
authors: Liu
authors: School of Chinese Medicine, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China.
authors: Rui-Yang
authors: RY
authors: Li
authors: School of Chinese Medicine, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China. Electronic address: jyliang08@126.com.
authors: Jing-Yu
authors: JY
authors: Liang
authors: Research Division of Pharmacology, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China. Electronic address: tyq@cpu.edu.cn.
authors: Yi-Qun
authors: YQ
authors: Tang
authors: Department of Biology, Georgia State University, Atlanta, GA 30303, USA. Electronic address: ychnjcpu@gmail.com.
authors: Chun-Hua
authors: CH
authors: Yang
mesh_terms: D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D004224:Diterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D015394:Molecular Structure; D032383:Myocytes, Cardiac / Q000187:drug effects; D018408:Patch-Clamp Techniques; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D012964:Sodium / Q000502:physiology
chemical_list: D000470:Alkaloids; D000889:Anti-Arrhythmia Agents; D004224:Diterpenes; D010936:Plant Extracts; C000594058:guan-fu base J; C000594059:guan-fu base N; C000594060:guan-fu base S; D012964:Sodium
keywords: Aconitum coreanum; Anti-arrhythmic effect; Diterpenoid alkaloid; Guan-Fu base J; Guan-Fu base N