title: Chemiluminescence immunoassay based on microfluidic chips for α-fetoprotein.
abstract: BACKGROUND
  Conventional immunoassays are labor intensive, time consuming, expensive and require large pieces of equipment for detection. In an effort to overcome these shortcomings, this study established an immunoassay method of alpha fetoprotein (AFP) in serum in combination with the microfluidic chip technology.
  
  
  METHODS
  A sandwich immunoassay approach was applied to detect AFP based on microfluidic chips and the chemiluminescence as detection signal. The chip used in this method was composed of a polydimethylsiloxane (PDMS) microchannel layer over a PDMS base layer.
  
  
  RESULTS
  AFP concentration and chemiluminescence intensity were linearly correlated over the concentration ranging from 12.5 to 200 ng/ml, and a detection limit as low as 1.5 ng/ml using this method. The coefficients of variation were 9.91% and 11.4% for the within- and between-run assays, respectively. More than 50 clinical samples were tested and the results obtained for this method strongly correlated with Roche's electrochemiluminescence (ECL) kit.
  
  
  CONCLUSIONS
  The proposed method offers a reliable, simple, reagent safe and inexpensive analytical platform for the determination of AFP in serum, and promotes the development of high throughput screening and point-of-care testing (POCT) diagnostics in clinical practice.
authors: Laboratory Diagnosis Center, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China.
authors: Fei
authors: F
authors: Fan
authors: CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing 100190, China.
authors: Haiying
authors: H
authors: Shen
authors: Laboratory Diagnosis Center, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China.
authors: Guojun
authors: G
authors: Zhang
authors: CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing 100190, China.
authors: Xingyu
authors: X
authors: Jiang
authors: Laboratory Diagnosis Center, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China. Electronic address: kangxx2013@163.com.
authors: Xixiong
authors: X
authors: Kang
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D057230:Limit of Detection; D049449:Luminescence; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D008904:Miniaturization; D011247:Pregnancy; D012016:Reference Values; D015203:Reproducibility of Results; D000509:alpha-Fetoproteins / Q000032:analysis*
chemical_list: D000509:alpha-Fetoproteins
keywords: Chemiluminescence; Microfluidic chips; Sandwich immunoassay; α-Fetoprotein