title: Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography.
abstract: Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-μm-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.
authors: Department of Physics, Arizona State University, Tempe, Arizona 85287, USA.
authors: Uwe
authors: U
authors: Weierstall
authors: Department of Physics, Arizona State University, Tempe, Arizona 85287, USA.
authors: Daniel
authors: D
authors: James
authors: The Scripps Research Institute, Department of Integrative Structural and Computational Biology, La Jolla, California 92037, USA.
authors: Chong
authors: C
authors: Wang
authors: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607, Germany.
authors: Thomas A
authors: TA
authors: White
authors: Department of Physics, Arizona State University, Tempe, Arizona 85287, USA.
authors: Dingjie
authors: D
authors: Wang
authors: The Scripps Research Institute, Department of Integrative Structural and Computational Biology, La Jolla, California 92037, USA.
authors: Wei
authors: W
authors: Liu
authors: Department of Physics, Arizona State University, Tempe, Arizona 85287, USA.
authors: John C H
authors: JC
authors: Spence
authors: Department of Physics, Arizona State University, Tempe, Arizona 85287, USA.
authors: R
authors: R
authors: Bruce Doak
authors: Department of Physics, Arizona State University, Tempe, Arizona 85287, USA.
authors: Garrett
authors: G
authors: Nelson
authors: Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, USA.
authors: Petra
authors: P
authors: Fromme
authors: Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, USA.
authors: Raimund
authors: R
authors: Fromme
authors: Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, USA.
authors: Ingo
authors: I
authors: Grotjohann
authors: Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, USA.
authors: Christopher
authors: C
authors: Kupitz
authors: Department of Physics, Arizona State University, Tempe, Arizona 85287, USA.
authors: Nadia A
authors: NA
authors: Zatsepin
authors: Department of Physics, Arizona State University, Tempe, Arizona 85287, USA.
authors: Haiguang
authors: H
authors: Liu
authors: Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, USA.
authors: Shibom
authors: S
authors: Basu
authors: The Scripps Research Institute, Department of Integrative Structural and Computational Biology, La Jolla, California 92037, USA.
authors: Daniel
authors: D
authors: Wacker
authors: The Scripps Research Institute, Department of Integrative Structural and Computational Biology, La Jolla, California 92037, USA.
authors: Gye Won
authors: GW
authors: Han
authors: The Scripps Research Institute, Department of Integrative Structural and Computational Biology, La Jolla, California 92037, USA.
authors: Vsevolod
authors: V
authors: Katritch
authors: SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
authors: Sébastien
authors: S
authors: Boutet
authors: SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
authors: Marc
authors: M
authors: Messerschmidt
authors: SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
authors: Garth J
authors: GJ
authors: Williams
authors: SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
authors: Jason E
authors: JE
authors: Koglin
authors: 1] SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA [2] Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-751 24, Sweden.
authors: M
authors: M
authors: Marvin Seibert
authors: 1] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607, Germany [2] Departamento de Química, Universidad Autónoma de Madrid, Ciudad Universitaria de Cantoblanco, Madrid 28049, Spain.
authors: Markus
authors: M
authors: Klinker
authors: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607, Germany.
authors: Cornelius
authors: C
authors: Gati
authors: Max-Planck-Institut für medizinische Forschung, Jahnstrasse 29, Heidelberg 69120, Germany.
authors: Robert L
authors: RL
authors: Shoeman
authors: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607, Germany.
authors: Anton
authors: A
authors: Barty
authors: 1] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607, Germany [2] Department of Physics, University of Hamburg, Hamburg 22761, Germany [3] Center for Ultrafast Imaging, Hamburg 22607, Germany.
authors: Henry N
authors: HN
authors: Chapman
authors: 1] Department of Physics, Arizona State University, Tempe, Arizona 85287, USA [2] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607, Germany.
authors: Richard A
authors: RA
authors: Kirian
authors: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607, Germany.
authors: Kenneth R
authors: KR
authors: Beyerlein
authors: The Scripps Research Institute, Department of Integrative Structural and Computational Biology, La Jolla, California 92037, USA.
authors: Raymond C
authors: RC
authors: Stevens
authors: School of Medicine and School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland.
authors: Dianfan
authors: D
authors: Li
authors: School of Medicine and School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland.
authors: Syed T A
authors: ST
authors: Shah
authors: School of Medicine and School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland.
authors: Nicole
authors: N
authors: Howe
authors: School of Medicine and School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland.
authors: Martin
authors: M
authors: Caffrey
authors: The Scripps Research Institute, Department of Integrative Structural and Computational Biology, La Jolla, California 92037, USA.
authors: Vadim
authors: V
authors: Cherezov
mesh_terms: D003461:Crystallography / Q000379:methods*; D008055:Lipids / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism
chemical_list: D008055:Lipids; D008565:Membrane Proteins; D043562:Receptors, G-Protein-Coupled