title: Comparison of Bush Sophora Root polysaccharide and its sulfate's anti-duck hepatitis A virus activity and mechanism.
abstract: In order to research the sulfating modification in enhancing the anti-duck hepatitis A virus (DHAV) activity of Bush Sophora Root polysaccharide (BSRPS), sulfated Bush Sophora Root polysaccharide (sBSRPS) was prepared by chlorosulfonic acid-pyridine method. KBr pellets method was applied to analyze their different structures. Anti-DHAV activity was studied by duck embryonic hepatocytes culture in vitro and artificial inoculation method in vivo. Direct immunofluorescence method and Real-time PCR were applied to study the antiviral mechanism of adsorption, replication and release in vitro and the dynamic change of virus content of blood in vivo. The results showed at the most effective content, sBSRPS (7.813 μg/mL) could inhibit both replication and release of DHAV in vitro, BSRPS (500 μg/mL) only inhibit replication. The relative expression of DHAV gene at the 8thh and the mortality rate of sBSRPS group were significantly reduced. These results indicated sBSRPS performed more effectively in anti-DHAV activity than BSRPS.
authors: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors: Yun
authors: Y
authors: Chen
authors: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors: Wen
authors: W
authors: Xiong
authors: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors: Ling
authors: L
authors: Zeng
authors: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors: Deyun
authors: D
authors: Wang
authors: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: liujiaguo@njau.edu.cn.
authors: Jiaguo
authors: J
authors: Liu
authors: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors: Yi
authors: Y
authors: Wu
authors: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors: Yuanliang
authors: Y
authors: Hu
mesh_terms: D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D017931:DNA Primers; D004354:Drug Screening Assays, Antitumor; D019085:Fluorescent Antibody Technique, Direct; D004371:Hepatitis Virus, Duck / Q000187:drug effects* / Q000502:physiology; D022781:Hepatocytes / Q000166:cytology / Q000821:virology; D018517:Plant Roots / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction; D029909:Sophora / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013431:Sulfates / Q000737:chemistry; D014779:Virus Replication / Q000187:drug effects
chemical_list: D017931:DNA Primers; D011134:Polysaccharides; D013431:Sulfates
keywords: Antiviral activity; Bush Sophora Root polysaccharide; Duck hepatitis A virus; Sulfated modification