title: A novel ribonuclease with HIV-1 reverse transcriptase inhibitory activity purified from the fungus Ramaria formosa.
abstract: A purification protocol that encompassed anion exchange chromatography (EC) on DEAE-cellulose, cation EC on CM-cellulose, anion EC on Q-Sepharose, and fast protein liquid chromatography-gel filtration of Superdex 75 was used to isolate a ribonuclease from dried fruiting bodies of Ramaria formosa. The ribonuclease was unadsorbed on CM-cellulose but adsorbed on both DEAE-cellulose and Q-Sepharose. It displayed a molecular mass of 29-kDa in both gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The ranking of its ribonucleolytic activity toward polyhomoribonucleotides was poly(U) > poly(C) > poly(G) > poly(A). It exhibited a pH optimum of pH 5 and a temperature optimum at 60 °C. The ribonuclease inhibited HIV-1 reverse transcriptase with an IC50 of 3 µM.
authors: State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing, China.
authors: Rui
authors: R
authors: Zhang
authors: Guoting
authors: G
authors: Tian
authors: Yongchang
authors: Y
authors: Zhao
authors: Liyan
authors: L
authors: Zhao
authors: Hexiang
authors: H
authors: Wang
authors: Zhiyuan
authors: Z
authors: Gong
authors: Tzi Bun
authors: TB
authors: Ng
mesh_terms: D000595:Amino Acid Sequence; D001487:Basidiomycota / Q000201:enzymology*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003636:DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D048690:Fruiting Bodies, Fungal / Q000201:enzymology; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012260:Ribonucleases / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D013624:Taiwan
chemical_list: D003636:DEAE-Cellulose; C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase; D012260:Ribonucleases
keywords: HIV-1 reverse transcriptase; Ramaria formosa; Ribonuclease