title: Methanogenic archaea diversity in Hanwoo (Bos taurus coreanae) rumen fluid, rectal dung, and barn floor manure using a culture-independent method based on mcrA gene sequences.
abstract: The diversity of methanogenic archaea associated with Korean Hanwoo cattle was analyzed using mcrA gene sequences from samples of rumen fluid (RF), rectal dung (RD), and barn floor manure (BFM). The predominant species were Methanobrevibacter ruminantium in RF and BFM(63.6% and 62.4%, respectively) and Methanocorpusculum labreanum in RD (53.2%).
authors: Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors: Aileen Rose
authors: AR
authors: Daquiado
authors: Departement of Food Science, Gyeongnam National University of Science and Technology, Jinju 660-758, Republic of Korea.
authors: Kye Man
authors: KM
authors: Cho
authors: Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors: Tae Young
authors: TY
authors: Kim
authors: Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju 660-701, Republic of Korea; Department of Animal Science (Institute of Agriculture and Life Science), Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors: Sam Churl
authors: SC
authors: Kim
authors: Department of Animal Science (Institute of Agriculture and Life Science), Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors: Hong-Hee
authors: HH
authors: Chang
authors: Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea. Electronic address: yblee5467@gmail.com.
authors: Yong Bok
authors: YB
authors: Lee
mesh_terms: D000818:Animals; D001105:Archaea / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D044822:Biodiversity; D002417:Cattle; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D008372:Manure / Q000382:microbiology*; D008697:Methane / Q000378:metabolism*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics; D012417:Rumen / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D019641:DNA, Archaeal; D008372:Manure; D010088:Oxidoreductases; C021572:methyl coenzyme M reductase; D008697:Methane
keywords: Dung; Manure; Methanogenic archaea; Rumen; mcrA gene