title: Graphene nano-ink biosensor arrays on a microfluidic paper for multiplexed detection of metabolites.
abstract: The development of a miniaturized and low-cost platform for the highly sensitive, selective and rapid detection of multiplexed metabolites is of great interest for healthcare, pharmaceuticals, food science, and environmental monitoring. Graphene is a delicate single-layer, two-dimensional network of carbon atoms with extraordinary electrical sensing capability. Microfluidic paper with printing technique is a low cost matrix. Here, we demonstrated the development of graphene-ink based biosensor arrays on a microfluidic paper for the multiplexed detection of different metabolites, such as glucose, lactate, xanthine and cholesterol. Our results show that the graphene biosensor arrays can detect multiple metabolites on a microfluidic paper sensitively, rapidly and simultaneously. The device exhibits a fast measuring time of less than 2 min, a low detection limit of 0.3 μM, and a dynamic detection range of 0.3-15 μM. The process is simple and inexpensive to operate and requires a low consumption of sample volume. We anticipate that these results could open exciting opportunities for a variety of applications.
authors: Department of Biological Engineering, Utah State University, Logan 84322, UT, USA.
authors: Pratima
authors: P
authors: Labroo
authors: Department of Biological Engineering, Utah State University, Logan 84322, UT, USA. Electronic address: yue.cui@usu.edu.
authors: Yue
authors: Y
authors: Cui
mesh_terms: D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation*; D001786:Blood Glucose / Q000032:analysis; D002784:Cholesterol / Q000097:blood; D004867:Equipment Design; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007281:Ink; D019344:Lactic Acid / Q000097:blood; D057230:Limit of Detection; D046228:Microarray Analysis / Q000191:economics / Q000295:instrumentation; D046210:Microfluidic Analytical Techniques / Q000191:economics / Q000295:instrumentation*; D010209:Paper; D019820:Xanthine / Q000097:blood
chemical_list: D001786:Blood Glucose; D019820:Xanthine; D019344:Lactic Acid; D006108:Graphite; D002784:Cholesterol
keywords: Biosensor arrays; Graphene ink; Metabolite; Microfluidic paper; Multiplexed