title: Imaging mGluR5 dynamics in astrocytes using quantum dots.
abstract: This unit describes the method that we have developed to clarify endogenous mGluR5 (metabotropic glutamate receptors 5) dynamics in astrocytes by single-particle tracking using quantum dots (QD-SPT). QD-SPT has been a powerful tool to examine the contribution of neurotransmitter receptor dynamics to synaptic plasticity. Neurotransmitter receptors are also expressed in astrocytes, the most abundant form of glial cell in the brain. mGluR5s, which evoke intracellular Ca(2+) signals upon receiving glutamate, contribute to the modulation of synaptic transmission efficacy and local blood flow by astrocytes. QD-SPT has previously revealed that the regulation of the lateral diffusion of mGluR5 on the plasma membrane is important for local Ca(2+) signaling in astrocytes. Determining how mGluR5 dynamics are regulated in response to neuronal input would enable a better understanding of neuron-astrocyte communication in future studies.
authors: Laboratory for Developmental Neurobiology, Brain Science Institute, Saitama, Japan.|Interdisciplinary Institute for Neuroscience (IINS), Université Bordeaux Segalen, Bordeaux, France.
authors: Misa
authors: M
authors: Arizono
authors: Laboratory for Developmental Neurobiology, Brain Science Institute, Saitama, Japan.|Division of Biological Sciences, Graduate School of Science, Nagoya University, Nagoya, Japan.
authors: Hiroko
authors: H
authors: Bannai
authors: Laboratory for Developmental Neurobiology, Brain Science Institute, Saitama, Japan.
authors: Katsuhiko
authors: K
authors: Mikoshiba
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000737:chemistry* / Q000502:physiology*; D020013:Calcium Signaling / Q000502:physiology; D002478:Cells, Cultured; D005260:Female; D005456:Fluorescent Dyes / Q000032:analysis; D006624:Hippocampus / Q000737:chemistry / Q000166:cytology / Q000502:physiology; D056004:Molecular Dynamics Simulation; D057054:Molecular Imaging / Q000379:methods*; D011247:Pregnancy; D045663:Quantum Dots / Q000032:analysis*; D051381:Rats; D064529:Receptor, Metabotropic Glutamate 5 / Q000032:analysis* / Q000502:physiology*
chemical_list: D005456:Fluorescent Dyes; C578937:Grm5 protein, rat; D064529:Receptor, Metabotropic Glutamate 5
keywords: astrocyte; mGluR5; quantum dot; single particle tracking