title: BioID: a screen for protein-protein interactions.
abstract: BioID is a unique method to screen for physiologically relevant protein interactions that occur in living cells. This technique harnesses a promiscuous biotin ligase to biotinylate proteins based on proximity. The ligase is fused to a protein of interest and expressed in cells, where it biotinylates proximal endogenous proteins. Because it is a rare protein modification in nature, biotinylation of these endogenous proteins by BioID fusion proteins enables their selective isolation and identification with standard biotin-affinity capture. Proteins identified by BioID are candidate interactors for the protein of interest. BioID can be applied to insoluble proteins, can identify weak and/or transient interactions, and is amenable to temporal regulation. Initially applied to mammalian cells, BioID has potential application in a variety of cell types from diverse species.
authors: Children's Health Research Center, Sanford Research, North Sioux Falls, South Dakota.|Department of Pediatrics, Sanford School of Medicine, University of South Dakota, Sioux Falls, South Dakota.
authors: Kyle J
authors: KJ
authors: Roux
authors: Children's Health Research Center, Sanford Research, North Sioux Falls, South Dakota.
authors: Dae In
authors: DI
authors: Kim
authors: Institute of Medical Biology, Immunos, Singapore.
authors: Brian
authors: B
authors: Burke
mesh_terms: D019921:Biotinylation / Q000379:methods*; D015151:Immunoblotting; D025941:Protein Interaction Mapping / Q000379:methods*; D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D011506:Proteins
keywords: BioID; biotinylation; protein-protein interaction; proximity-dependent labeling