title: Serum levels of miR-19b and miR-146a as prognostic biomarkers for non-small cell lung cancer.
abstract: MicroRNA (miRNA) is a type of small non-coding RNA molecule that has important roles in cancer initiation, promotion and progression by negatively regulating gene expression. In this study, we explored the role of miRNAs in the prognosis of patients with non-small cell lung cancer (NSCLC). The miRNA expression profiles were determined in 5 pairs of NSCLC and paracancerous tissues (3 adenocarcinomas and 2 squamous cell carcinomas). Aberrantly expressed miRNAs were validated by quantitative real-time PCR (qRT-PCR) in 61 pairs of NSCLC and paracancerous tissues. Differentially expressed miRNAs were further analyzed in sera from 94 healthy subjects and 94 advanced NSCLC patients receiving platinum-based chemotherapy. Three miRNAs (miR-19b, miR-146a, and miR-223) were significantly dysregulated in NSCLC tissues (P < 0.05). High miR-19b and low miR-146a expression in NSCLC tissues were associated with higher TNM stage, lymph node metastasis and poorer survival (P < 0.05). The serum levels of miR-19b in NSCLC patients were significantly higher (P < 0.001), whereas serum levels of miR-146a were significantly lower (P < 0.001), compared with those in controls. Serum levels of miR-19b and miR-146a were associated with overall survival of NSCLC patients (P < 0.05). Patients with low serum level of miR-19b and high serum level of miR-146a achieved a higher overall response rate and longer survival time (P < 0.05). These data suggest that miR-19b and miR-146a are potential biomarkers for the prediction of survival and response to chemotherapy in NSCLC.
authors: Department of Thoracic Surgery, the First Affiliated Hospital of Xiamen University.
authors: Chaohui
authors: C
authors: Wu
authors: Yunpeng
authors: Y
authors: Cao
authors: Zefeng
authors: Z
authors: He
authors: Jianbing
authors: J
authors: He
authors: Chao
authors: C
authors: Hu
authors: Hongbing
authors: H
authors: Duan
authors: Jie
authors: J
authors: Jiang
mesh_terms: D000230:Adenocarcinoma / Q000097:blood / Q000175:diagnosis*; D014408:Biomarkers, Tumor / Q000097:blood*; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood / Q000175:diagnosis*; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000175:diagnosis*; D002681:China; D020869:Gene Expression Profiling; D006801:Humans; D008207:Lymphatic Metastasis / Q000175:diagnosis*; D035683:MicroRNAs / Q000097:blood; D060888:Real-Time Polymerase Chain Reaction; D012044:Regression Analysis
chemical_list: D014408:Biomarkers, Tumor; C542542:MIRN146 microRNA, human; C549214:MIRN19A microRNA, human; D035683:MicroRNAs