title: Engrailed-2 is down-regulated but also ectopically expressed in clear cell renal cell carcinoma.
abstract: Engrailed-2 (EN2), a member of the homeobox family of genes, encodes a homeodomain-containing transcription factor that is thought to be a potential oncogene in a number of cancers. Because the role of EN2 in clear cell renal cell carcinoma (CCRCC) has not been determined, we investigated its expression in CCRCC tissues and cell lines. Using immunohistochemical (IHC) staining, we found that EN2 protein was expressed in normal renal cells and tubules, but was frequently down-regulated in tissues from patients with CCRCC and in CCRCC cell lines. In addition, we found that EN2, which functions in the nucleus, was completely localized to the cytoplasm of CCRCC cells as detected by IHC and immunofluorescence staining. Furthermore, expression of EN2 protein was negatively correlated with increasing histological grade of CCRCC tumors (P = 0.003). The exact role of EN2 expression in renal carcinoma carcinogenesis requires further investigation.
authors: Department of Urology, The First Affiliated Hospital of Jinan University, No. 613 Huang Pu Rd, Guangzhou, 510630, China.
authors: Cai-yong
authors: CY
authors: Lai
authors: Bin
authors: B
authors: Pan
authors: Yun
authors: Y
authors: Luo
authors: Wei-bo
authors: WB
authors: Liang
authors: Jie
authors: J
authors: Chen
authors: Dong-ming
authors: DM
authors: Ye
authors: Ji-Nan
authors: JN
authors: Guo
authors: Li
authors: L
authors: Li
authors: Ze-xuan
authors: ZX
authors: Su
mesh_terms: D000328:Adult; D000368:Aged; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D000099266:Engrailed 2 Protein
chemical_list: D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; D000099266:Engrailed 2 Protein