title: The protective effects of tacrolimus on rat uteri exposed to ischemia-reperfusion injury: a biochemical and histopathologic evaluation.
abstract: OBJECTIVE
  To evaluate the effects of the immunosuppressant tacrolimus as an antioxidant and analyze the histopathologic changes in rat uteri exposed to experimental ischemia-reperfusion (I/R) injury.
  
  
  METHODS
  Experimental study.
  
  
  METHODS
  Experimental surgery laboratory in a university.
  
  
  METHODS
  Twenty-eight female rats exposed to experimentally induced uterine I/R injury.
  
  
  METHODS
  Group I: control group; group II: uterine I/R injury-induced group; group III: pre-ischemia tacrolimus group; group IV: post-ischemia tacrolimus group.
  
  
  METHODS
  Uterine tissue malondialdehyde (MDA) level as a marker of lipid peroxidation and glutathione (GSH) level and superoxide dismutase (SOD) and catalase (CAT) activities as markers of tissue antioxidant capacity; histopathologic examination of all uterine rat tissue.
  
  
  RESULTS
  Following aortic I/R injury, MDA levels were significantly increased whereas GSH levels and CAT and SOD activities were found to be decreased compared with control animals. MDA levels were found to recover prominently after the administration of tacrolimus in both groups III and IV. Administration of tacrolimus improved uterine GSH levels and CAT activity in the tacrolimus-treated groups.
  
  
  CONCLUSIONS
  Our results indicate that tacrolimus reduces oxidative damage in rat uteri exposed to I/R injury induced by distal abdominal aortic occlusion. Histologic evaluation reveals that tacrolimus attenuates the inflammatory response and protects the tissue damage induced by I/R injury.
authors: Department of Obstetrics and Gynecology, Zeynep Kamil Gynecologic and Pediatric Training and Research Hospital, Istanbul, Turkey. Electronic address: drsadiksahin@gmail.com.
authors: Sadik
authors: S
authors: Sahin
authors: Department of Biochemistry, School of Pharmacy, Marmara University, Istanbul, Turkey.
authors: Ozlem Bingol
authors: OB
authors: Ozakpinar
authors: Department of Cardiovascular Surgery, Marmara University, Istanbul, Turkey.
authors: Koray
authors: K
authors: Ak
authors: Department of Obstetrics and Gynecology, Zeynep Kamil Gynecologic and Pediatric Training and Research Hospital, Istanbul, Turkey.
authors: Mustafa
authors: M
authors: Eroglu
authors: Department of Histology and Embryology, School of Medicine, Marmara University, Istanbul, Turkey.
authors: Merve
authors: M
authors: Acikel
authors: Department of Biochemistry, School of Pharmacy, Marmara University, Istanbul, Turkey.
authors: Sermin
authors: S
authors: Tetik
authors: Department of Biochemistry, School of Pharmacy, Marmara University, Istanbul, Turkey.
authors: Fikriye
authors: F
authors: Uras
authors: Department of Histology and Embryology, School of Medicine, Marmara University, Istanbul, Turkey.
authors: Sule
authors: S
authors: Cetinel
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D004716:Endometritis / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology*; D016559:Tacrolimus / Q000627:therapeutic use*; D016896:Treatment Outcome
chemical_list: D000975:Antioxidants; D007166:Immunosuppressive Agents; D016559:Tacrolimus
keywords: Ischemia-reperfusion injury; rat; tacrolimus; transplantation; uterus