title: Tumor-homing glycol chitosan-based optical/PET dual imaging nanoprobe for cancer diagnosis.
abstract: Imaging techniques including computed tomography, magnetic resonance imaging, and positron emission tomography (PET) offer many potential benefits to diagnosis and treatment of cancers. Each method has its own strong and weak points. Therefore, multimodal imaging techniques have been highlighted as an alternative method for overcoming the limitations of each respective imaging method. In this study, we fabricated PET/optical activatable imaging probe based on glycol chitosan nanoparticles (CNPs) for multimodal imaging. To prepare the dual PET/optical probes based on CNPs, both (64)Cu radiolabeled DOTA complex and activatable matrix metalloproteinase (MMP)-sensitive peptide were chemically conjugated onto azide-functionalized CNPs via bio-orthogonal click chemistry, which was a reaction between azide group and dibenzyl cyclooctyne. The PET/optical activatable imaging probes were visualized by PET and optical imaging system. Biodistribution of probes and activity of MMP were successfully measured in tumor-bearing mice.
authors: Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology (KIST) , Seoul 136-791, Republic of Korea.
authors: Sangmin
authors: S
authors: Lee
authors: Sun-Woong
authors: SW
authors: Kang
authors: Ju Hee
authors: JH
authors: Ryu
authors: Jin Hee
authors: JH
authors: Na
authors: Dong-Eun
authors: DE
authors: Lee
authors: Seung Jin
authors: SJ
authors: Han
authors: Choong Mo
authors: CM
authors: Kang
authors: Yearn Seong
authors: YS
authors: Choe
authors: Kyo Chul
authors: KC
authors: Lee
authors: James F
authors: JF
authors: Leary
authors: Kuiwon
authors: K
authors: Choi
authors: Kyung-Han
authors: KH
authors: Lee
authors: Kwangmeyung
authors: K
authors: Kim
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry; D003301:Copper Radioisotopes; D006018:Glycols / Q000737:chemistry; D006801:Humans; D061986:MCF-7 Cells; D008297:Male; D020782:Matrix Metalloproteinases / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology; D009374:Neoplasms, Experimental / Q000175:diagnosis* / Q000201:enzymology / Q000378:metabolism; D055096:Optical Devices; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D049268:Positron-Emission Tomography; D019275:Radiopharmaceuticals / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D003301:Copper Radioisotopes; D006018:Glycols; D010455:Peptides; D019275:Radiopharmaceuticals; D048271:Chitosan; D020782:Matrix Metalloproteinases