title: An impedimetric immunosensor based on diamond nanowires decorated with nickel nanoparticles.
abstract: Nanostructured boron-doped diamond has been investigated as a sensitive impedimetric electrode for the detection of immunoglobulin G (IgG). The immunosensor was constructed in a three-step process: (i) reactive ion etching of flat boron-doped diamond (BDD) interfaces to synthesize BDD nanowires (BDD NWs), (ii) electrochemical deposition of nickel nanoparticles (Ni NPs) on the BDD NWs, and (iii) immobilization of biotin-tagged anti-IgG onto the Ni NPs. Electrochemical impedance spectroscopy (EIS) was used to follow the binding of IgG at different concentrations without the use of any additional label. A detection limit of 0.3 ng mL(-1) (2 nM) with a dynamic range up to 300 ng mL(-1) (2 μM) was obtained with the interface. Moreover, the study demonstrated that this immunosensor exhibits good stability over time and allows regeneration by incubation in ethylenediaminetetraacetic acid (EDTA) aqueous solution.
authors: Institut de Recherche Interdisciplinaire (IRI), CNRS USR 3078, Université Lille1, Parc de la Haute Borne, 50 avenue de Halley, BP 70478, 59658 Villeneuve d'Ascq, France.
authors: Palaniappan
authors: P
authors: Subramanian
authors: Anastasiia
authors: A
authors: Motorina
authors: Weng Siang
authors: WS
authors: Yeap
authors: Ken
authors: K
authors: Haenen
authors: Yannick
authors: Y
authors: Coffinier
authors: Vladimir
authors: V
authors: Zaitsev
authors: Joanna
authors: J
authors: Niedziolka-Jonsson
authors: Rabah
authors: R
authors: Boukherroub
authors: Sabine
authors: S
authors: Szunerits
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000737:chemistry; D055910:Antibodies, Immobilized; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001895:Boron / Q000737:chemistry*; D002417:Cattle; D018130:Diamond / Q000737:chemistry*; D058266:Dielectric Spectroscopy / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D007074:Immunoglobulin G / Q000032:analysis*; D057230:Limit of Detection; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry; D053770:Nanowires / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry; D056951:Photoelectron Spectroscopy; D011817:Rabbits; D013499:Surface Properties
chemical_list: D000888:Antibodies, Anti-Idiotypic; D055910:Antibodies, Immobilized; D007074:Immunoglobulin G; C048844:anti-IgG; D018130:Diamond; D009532:Nickel; D001895:Boron