title: The effects of sleep deprivation on microRNA expression in rats submitted to pilocarpine-induced status epilepticus.
abstract: Epilepsy is a neurological disorder with significant prevalence and the individuals affected by this disease have a great probability of occurrence of a lethal phenomenon known as Sudden Unexpected Death in Epilepsy (SUDEP). SUDEP occurs mainly during the night and probably during sleep. The pathophysiological mechanisms involved in this lethal phenomenon are still obscure and new evidences that could corroborate in this area are warranted. Thus, the aim of the present study was to evaluate the effect of sleep deprivation in the expression of microRNA (miRNA) in the frontal cortex and heart tissues of adult male rats after 50days of saline (SAL) or pilocarpine-induced status epilepticus (PILO). Initially 389 miRNA expressions were evaluated between SAL and PILO groups by microarray. Subsequently, 3 differentially expressed miRNAs of each tissue were investigated after total sleep deprivation (TSD 6h) and paradoxical sleep deprivation (PSD 24h). Still, it was analyzed that the effects of sleep rebound with equivalent duration of PSD and TSD. There was a significant increase of miR-146a expression, an important inflammatory modulator in the frontal cortex of PILO rats when compared to SAL animals. Animals treated with pilocarpine were affected by TSD (through overexpression of miRNAs related to inflammatory process) and these changes were maintained even after a sleep window of 6h. In contrast, miRNAs associated with heart diseases were down-regulated in PSD rebound, suggesting a possible restoration of homeostasis in cardiovascular system of SAL and PILO groups.
authors: Departamento de Psicobiologia, Universidade Federal de São Paulo, Brazil.
authors: Gabriela
authors: G
authors: Matos
authors: Departamento de Neurologia Experimental, Universidade Federal de São Paulo, Brazil.
authors: Fulvio A
authors: FA
authors: Scorza
authors: Departamento de Psicobiologia, Universidade Federal de São Paulo, Brazil.
authors: Diego R
authors: DR
authors: Mazzotti
authors: Departamento de Psicobiologia, Universidade Federal de São Paulo, Brazil.
authors: Camila
authors: C
authors: Guindalini
authors: Departamento de Neurologia Experimental, Universidade Federal de São Paulo, Brazil.
authors: Esper A
authors: EA
authors: Cavalheiro
authors: Departamento de Psicobiologia, Universidade Federal de São Paulo, Brazil.
authors: Sergio
authors: S
authors: Tufik
authors: Departamento de Psicobiologia, Universidade Federal de São Paulo, Brazil. Electronic address: ml.andersen12@gmail.com.
authors: Monica L
authors: ML
authors: Andersen
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D004195:Disease Models, Animal; D005625:Frontal Lobe / Q000187:drug effects / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D046228:Microarray Analysis; D018721:Muscarinic Agonists / Q000633:toxicity*; D010862:Pilocarpine / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D012892:Sleep Deprivation / Q000378:metabolism*; D013226:Status Epilepticus / Q000139:chemically induced* / Q000503:physiopathology; D013997:Time Factors
chemical_list: D035683:MicroRNAs; D018721:Muscarinic Agonists; D010862:Pilocarpine
keywords: Epilepsy; MicroRNA; Pilocarpine; Rats; Sleep deprivation; Sudden unexpected death in epilepsy