title: [SPR-biosensor assay for analysis of small compounds interaction with human cytochrome P450 51A1 (CYP51A1)].
abstract: The SPR assay for human cytochrome P450 51A1's (CYP51A1) ligand screening was developed. Assay has been validated with known azole inhibitors of cytochrome P450s. The studied azoles selectively interacted with human cytochrome P450 51A1, which showed the highest affinity towards ketoconazole. The efficiency of the SPR assay was showed with 19 steroid and triterpene compounds, which were not investigated as potential ligands of CYP51A1.
authors: O V
authors: OV
authors: Gnedenko
authors: L A
authors: LA
authors: Kaluzhskiĭ
authors: A A
authors: AA
authors: Mol'nar
authors: A V
authors: AV
authors: Iantsevich
authors: D V
authors: DV
authors: Mukha
authors: A A
authors: AA
authors: Gilep
authors: S A
authors: SA
authors: Usanov
authors: V A
authors: VA
authors: Stonik
authors: A S
authors: AS
authors: Ivanov
authors: A V
authors: AV
authors: Lisitsa
authors: A I
authors: AI
authors: Archakov
mesh_terms: D058888:14-alpha Demethylase Inhibitors / Q000737:chemistry* / Q000378:metabolism; D015374:Biosensing Techniques; D006801:Humans; D007654:Ketoconazole / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D058886:Sterol 14-Demethylase / Q000737:chemistry* / Q000378:metabolism
chemical_list: D058888:14-alpha Demethylase Inhibitors; C548418:CYP51A1 protein, human; D058886:Sterol 14-Demethylase; D007654:Ketoconazole