title: Interferon lambda 1 expression in cervical cells differs between low-risk and high-risk human papillomavirus-positive women.
abstract: Persistent infection by high-risk (HR) human papillomavirus (HPV) types is a prerequisite for progression to cancer. HR-HPVs may lead to a deregulation of innate immunity by interfering with the epithelial type I interferon (IFN) response, whereas very little is known about type III IFNs, a key component of the mucosal antiviral response. This study reports a first attempt to evaluate the activation of type III IFN genes (IFN lambda 1-3), IFN lambda receptor genes (IFN-lambdaR1 and IL10R2), and IFN-induced genes (MxA, ISG15, ISG56) in HPV-positive and HPV-negative cervical cells from 154 women attending the gynecological unit of a university hospital in Rome. Despite an increased individual variability, a coordinated expression of several IFN lambda-related genes was observed. Furthermore, IFN lambda 1 and IFN-lambdaR1 genes were expressed at higher levels in cervical cells positive to low-risk (LR) HPV compared to HR-HPV and HPV-negative cells. Consistently, ISG15 expression was significantly higher in LR-HPV-infected women than in the other groups. Moreover, IFN lambda 1 expression decreased significantly with abnormal cytological results. This study is the first to show the activation of a type III IFN response in LR-HPV-positive cervical cells and suggests that the lack of this response in HR-HPV infection may be related to lesion progression.
authors: Laboratory of Virology, Department of Molecular Medicine, 'Sapienza' University, Viale di Porta, Tiburtina 28, 00185, Rome, Italy.
authors: Fabiana
authors: F
authors: Cannella
authors: Carolina
authors: C
authors: Scagnolari
authors: Carla
authors: C
authors: Selvaggi
authors: Patrizia
authors: P
authors: Stentella
authors: Nadia
authors: N
authors: Recine
authors: Guido
authors: G
authors: Antonelli
authors: Alessandra
authors: A
authors: Pierangeli
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D020869:Gene Expression Profiling; D006785:Hospitals, University; D006801:Humans; D007372:Interferons; D007378:Interleukins / Q000096:biosynthesis*; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification* / Q000276:immunology* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000276:immunology* / Q000821:virology*; D018121:Receptors, Cytokine / Q000096:biosynthesis*; D017471:Receptors, Interferon; D012384:Rome; D055815:Young Adult; D000095022:Interferon Lambda
chemical_list: D007372:Interferons; D007378:Interleukins; D018121:Receptors, Cytokine; D017471:Receptors, Interferon; C000624353:interferon-lambda, human; D000095022:Interferon Lambda; C579640:IFNLR1 protein, human