title: Association of plasma soluble α-klotho with pro-endothelin-1 in patients with type 2 diabetes.
abstract: OBJECTIVE
  To study the relationship between plasma soluble klotho (sKlotho) and pro-endothelin-1 (proET-1) in patients with type 2 diabetes (T2DM).
  
  
  METHODS
  In this cross-sectional study, we recruited 175 T2DM subjects and 56 non-diabetic controls. Plasma sKlotho, proET-1 and extracellular superoxide dismutase (SOD) were measured by ELISA and ILMA, respectively.
  
  
  RESULTS
  Plasma sKlotho level in patients with T2DM was lower compared to that in non-diabetic controls (416.8±148.1 vs. 494.6±134.3 pg/ml, p=0.001) and showed significant interaction with diabetes status in its association with proET-1. Plasma sKlotho was inversely correlated with proET-1 in T2DM (Rho=-0.410, p<0.0001) but not in non-diabetic controls (Rho=0.091, p=0.505). Multivariable linear regression models revealed that sKlotho was independently associated with proET-1 after adjustment for renal filtration function, albuminuria, diabetes duration, HbA1c, systolic and diastolic blood pressure.
  
  
  CONCLUSIONS
  Plasma sKlotho was associated with proET-1 independent of renal function in patients with T2DM.
authors: Clinical Research Unit, Khoo Teck Puat Hospital, Republic of Singapore.
authors: Jian-Jun
authors: JJ
authors: Liu
authors: Clinical Research Unit, Khoo Teck Puat Hospital, Republic of Singapore.
authors: Sylvia
authors: S
authors: Liu
authors: Institut für Experimentelle Endokrinologie, Charité Universitätsmedizin Berlin, Germany.
authors: Nils G
authors: NG
authors: Morgenthaler
authors: Clinical Research Unit, Khoo Teck Puat Hospital, Republic of Singapore.
authors: Melvin D S
authors: MDS
authors: Wong
authors: Diabetes Centre, Khoo Teck Puat Hospital, 90 Yishun Central, Singapore 768828, Republic of Singapore.
authors: Subramaniam
authors: S
authors: Tavintharan
authors: Diabetes Centre, Khoo Teck Puat Hospital, 90 Yishun Central, Singapore 768828, Republic of Singapore.
authors: Chee Fang
authors: CF
authors: Sum
authors: Diabetes Centre, Khoo Teck Puat Hospital, 90 Yishun Central, Singapore 768828, Republic of Singapore. Electronic address: lim.su.chi@alexandrahealth.com.sg.
authors: Su Chi
authors: SC
authors: Lim
mesh_terms: D000368:Aged; D000419:Albuminuria / Q000209:etiology; D001786:Blood Glucose / Q000032:analysis; D001794:Blood Pressure; D015992:Body Mass Index; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D003925:Diabetic Angiopathies / Q000097:blood; D003928:Diabetic Nephropathies / Q000097:blood / Q000652:urine; D003929:Diabetic Neuropathies / Q000097:blood; D019332:Endothelin-1 / Q000097:blood*; D005260:Female; D005919:Glomerular Filtration Rate; D005966:Glucuronidase / Q000097:blood*; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D000090265:Klotho Proteins; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D011498:Protein Precursors / Q000097:blood*; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D001786:Blood Glucose; D019332:Endothelin-1; D005966:Glucuronidase; D006442:Glycated Hemoglobin; D000090265:Klotho Proteins; D008055:Lipids; D011498:Protein Precursors; D013482:Superoxide Dismutase; C093240:proendothelin 1
keywords: Endothelin-1; Oxidative stress; Soluble klotho; Type 2 diabetes; Vascular complication