title: BD GeneOhm-MRSA assay for detection of methicillin-resistant Staphylococcus aureus directly in nasal and non-nasal swab specimens from haematologic patients.
abstract: Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) are responsible for rising health care costs and have a high attribution to mortality. Reliable and rapid detection of MRSA carriage is essential. Real-time PCR allows an early detection of MRSA colonization within 2 h. By using the BD GeneOhm-MRSA assay we analysed directly swabs of different sampling sites and compared the assay with culture method. One thousand one hundred and sixty samples from 129 patients in Magdeburg were examined. Of the samples, 8 (0.69%) or 1117 (96.3%) were tested equally positive or negative by both methods whereas 16 (1.38%) specimens were MRSA positive only by the GeneOhm-MRSA assay and 6 (0.52%) were MRSA positive only by culture method. Thirteen samples (1.12%), which are culture negative, were unresolved by the GeneOhm-MRSA. With regard to the patients, seven were detected as MRSA carriers only by the GeneOhm-MRSA while one patient was tested positive for MRSA only by culture. Assuming 100% correct results by the culture method, sensitivity and specificity of GeneOhm-MRSA assay could be calculated as 84.4% and 96.1% for nasal swabs, 78.7% and 96.9% for all swabs under study, and 94.8% and 99.5% when focussed on patients. PPV and NPV were 70.3% and 98% for all specimens together, respectively. BD GeneOhm-MRSA assay is a sensitive test for the detection of MRSA colonization from swab specimens without the need for an initial culture, but should always be performed in parallel to the culture method for comparison reasons. Furthermore, our results indicate that in addition swabs taken from different body sites were successfully analysed by the BD GeneOhm-MRSA assay. However, we conclude that the PCR assay might not be a preferred tool for screening in haematologic patients with low MRSA rate; for screening haematologic patients, the culture method is sufficient enough.
authors: Otto-von-Guericke-University Clinic, Clinical Microbiology Magdeburg Germany.
authors: B
authors: B
authors: Ghebremedhin
authors: Otto-von-Guericke-University Clinic, Clinical Microbiology Magdeburg Germany.
authors: B
authors: B
authors: König
authors: Otto-von-Guericke-University Clinic, Clinical Microbiology Magdeburg Germany.
authors: W
authors: W
authors: König
keywords: MRSA; haematology; non-nasal specimens; real-time PCR; screening