title: Characterization of HelD, an interacting partner of RNA polymerase from Bacillus subtilis.
abstract: Bacterial RNA polymerase (RNAP) is an essential multisubunit protein complex required for gene expression. Here, we characterize YvgS (HelD) from Bacillus subtilis, a novel binding partner of RNAP. We show that HelD interacts with RNAP-core between the secondary channel of RNAP and the alpha subunits. Importantly, we demonstrate that HelD stimulates transcription in an ATP-dependent manner by enhancing transcriptional cycling and elongation. We demonstrate that the stimulatory effect of HelD can be amplified by a small subunit of RNAP, delta. In vivo, HelD is not essential but it is required for timely adaptations of the cell to changing environment. In summary, this study establishes HelD as a valid component of the bacterial transcription machinery.
authors: Laboratory of Molecular Genetics of Bacteria, Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague 14220, Czech Republic, Department of Genetics and Microbiology, Faculty of Science, Charles University in Prague, Prague 12843, Czech Republic, Department of Structure Analysis of Biomacromolecules, Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague 16206, Czech Republic, Laboratory of Structure and Function of Biomolecules, Institute of Biotechnology, Academy of Sciences of the Czech Republic, Prague 14220, Czech Republic, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia and Laboratory of Molecular Structure Characterization, Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague 14220, Czech Republic.
authors: Jana
authors: J
authors: Wiedermannová
authors: Petra
authors: P
authors: Sudzinová
authors: Tomaš
authors: T
authors: Kovaľ
authors: Alžbeta
authors: A
authors: Rabatinová
authors: Hana
authors: H
authors: Šanderova
authors: Olga
authors: O
authors: Ramaniuk
authors: Šimon
authors: Š
authors: Rittich
authors: Jan
authors: J
authors: Dohnálek
authors: Zhihui
authors: Z
authors: Fu
authors: Petr
authors: P
authors: Halada
authors: Peter
authors: P
authors: Lewis
authors: Libor
authors: L
authors: Krásny
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D010641:Phenotype; D061805:Transcription Elongation, Genetic; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D000255:Adenosine Triphosphate; D004247:DNA; D012321:DNA-Directed RNA Polymerases