title: Evaluation of the Effect of miR-26b Up-Regulation on HbF Expression in Erythroleukemic K-562 Cell Line.
abstract: BACKGROUND
  The major hemoglobin in the fetus is hemoglobin F (HbF) (α2γ2), whereas in adult humans, hemoglobin A (α2β2) is predominately expressed. Several studies have indicated that expression of the HbF subunit γ-globin might be regulated post-transcriptionally. This could be done by small non-coding RNAs called microRNAs which target mRNAs in a sequence-specific manner and lead to translational repression or mRNA decay. The aim of this study is to evaluate the effect of miR-26b up-regulation on γ-globin gene expression in K-562 cell line.
  
  
  METHODS
  These cells were grown in RPMI 1640 and pre miR-26b and were transfected within K-562 cell line using lentiviral vector. After RNA extraction and cDNA synthesis in selected days, miRNA up-regulation was confirmed by miRNA real time PCR and then γand βchain and GATA-1 expression were investigated by RT and QRT-PCR.
  
  
  RESULTS
  The viability of cells before transfection was 90%. Three and 7 days after transfection, through the use of relative Q-PCR, the γ chain expression increased 3.7, 6.8 and 3.8 folds and GATA-1 expression increased 2.1, 6.0 and 8.0 in comparison with untransfected cells.
  
  
  CONCLUSIONS
  The data suggest that miR-26b can be involved in the increase of γ-globin gene expression in K-562 cell line. We suggest that miR-26b may be a significant therapeutic target for increasing HbF levels in patients with sickle cell disease and β-thalassemia.
authors: Department of Hematology, Allied Medical School, Tehran University of Medical Sciences, Tehran, Iran.
authors: Sadegh
authors: S
authors: Alijani
authors: Department of Hematology, Allied Medical School, Tehran University of Medical Sciences, Tehran, Iran.
authors: Shaban
authors: S
authors: Alizadeh
authors: Department of Hematology, Allied Medical School, Tehran University of Medical Sciences, Tehran, Iran.
authors: Ahmad
authors: A
authors: Kazemi
authors: Students Scientific Research Center (SSRC), Allied Medical School, Tehran University of Medical Sciences, Tehran, Iran.
authors: Zahra Kashani
authors: ZK
authors: Khatib
authors: Department of Hematology, Tarbiat Modares University, Tehran, Iran.
authors: Masoud
authors: M
authors: Soleimani
authors: Department of Hematology, Allied Medical School, Tehran University of Medical Sciences, Tehran, Iran.
authors: Mohamadreza
authors: M
authors: Rezvani
authors: Department of Hematology, Allied Medical School, Tehran University of Medical Sciences, Tehran, Iran.
authors: Neda
authors: N
authors: Minayi
authors: Department of Hematology, Allied Medical School, Tehran University of Medical Sciences, Tehran, Iran.
authors: Farshid
authors: F
authors: Karami
authors: Department of Hematology, Qaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
authors: Behnoosh
authors: B
authors: Tayebi
keywords: K-562; MicroRNAs; miR-26b