title: Polyphenols-rich Cyamopsis tetragonoloba (L.) Taub. beans show hypoglycemic and β-cells protective effects in type 2 diabetic rats.
abstract: The aim of this study was to evaluate the antidiabetic activity of Cyamopsis tetragonoloba (L.) Taub. (Fabaceae) beans in high-fat diet (HFD) fed-streptozotocin (STZ)-induced type 2 diabetic rats. Dose dependent response of oral treatment of C. tetragonoloba beans' methanol extract (CTme) (200 and 400mg/kg b wt.) was assessed by measuring fasting blood glucose, changes in body weight, plasma insulin, homeostasis model assessment of insulin resistance (HOMA-IR), total cholesterol, triglycerides, oral glucose tolerance, intraperitoneal insulin tolerance, hepatic glycogen, marker enzymes of carbohydrate metabolism in HFD fed-STZ-induced type 2 diabetic rats. Histology and immunohistochemical analysis of pancreatic islets were also performed. High-performance liquid chromatography (HPLC) analysis of CTme showed the presence of polyphenols such as gallic acid and caffeic acid in the concentrations of 2.46% (W/W) and 0.32% (W/W). CTme significantly reverted the altered biochemical parameters to near normal levels in diabetic rats. Furthermore CTme showed the protective effect on the β-cells of pancreatic tissues in diabetic rats. These findings indicate that C. tetragonoloba beans have therapeutic potential in HFD fed-STZ-induced hyperglycemia; therefore this can be used in the management of type 2 diabetes.
authors: Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Chennai 600034, India.
authors: Gopalsamy Rajiv
authors: GR
authors: Gandhi
authors: Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Chennai 600034, India.
authors: Pautu
authors: P
authors: Vanlalhruaia
authors: Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Chennai 600034, India.
authors: Antony
authors: A
authors: Stalin
authors: Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Chennai 600034, India.
authors: Santiagu Stephen
authors: SS
authors: Irudayaraj
authors: Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Chennai 600034, India. Electronic address: entolc@hotmail.com.
authors: Savarimuthu
authors: S
authors: Ignacimuthu
authors: Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Chennai 600034, India.
authors: Michael Gabriel
authors: MG
authors: Paulraj
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D031293:Cyamopsis / Q000737:chemistry*; D003924:Diabetes Mellitus, Type 2 / Q000517:prevention & control*; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007328:Insulin / Q000097:blood; D007515:Islets of Langerhans / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats
chemical_list: D001786:Blood Glucose; D007004:Hypoglycemic Agents; D007328:Insulin; D010936:Plant Extracts
keywords: Biochemical parameters; Cyamopsis tetragonoloba; Histology; Immunohistochemical; Polyphenols; Type 2 diabetes