title: Purification and characterization of antioxidative peptides from round scad (Decapterus maruadsi) muscle protein hydrolysate.
abstract: Muscle protein from round scad (Decapterus maruadsi) was hydrolyzed with five commercial proteases, namely, Alcalase, neutral protease, papain, pepsin, and trypsin. Round scad hydrolysate (RSH) prepared with Alcalase demonstrated high antioxidative activity. After ultrafiltration, RSH-III fraction (MW<5kDa) exhibited the strongest activity. Then, RSH-III was purified by gel filtration chromatography (Sephadex G-15) and separated into four fractions (A, B, C, and D), of which fraction B showed the highest antioxidative activity and was further purified using reverse-phase high-performance liquid chromatography twice. The purified peptides were identified as His-Asp-His-Pro-Val-Cys (706.8Da) and His-Glu-Lys-Val-Cys (614.7Da) by matrix-assisted laser desorption ionization time-of-flight/time-of-flight mass spectrometry. Subsequently, the identified peptides were synthesized, and their antioxidative activities were verified. Results indicated that the two novel peptides isolated from round scad muscle protein can be developed into antioxidative ingredients in functional foods.
authors: School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, Guangxi, China; Guangxi Key Lab of Petrochemical Resource Processing and Process Intensification Technology, Nanning 530004, Guangxi, China.
authors: Haiping
authors: H
authors: Jiang
authors: School of Chemistry and Materials Science, University of Science and Technology of China, Anhui 230026, China.
authors: Tianzhe
authors: T
authors: Tong
authors: School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, Guangxi, China.
authors: Jianhua
authors: J
authors: Sun
authors: School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, Guangxi, China.
authors: Yuanjin
authors: Y
authors: Xu
authors: School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, Guangxi, China.
authors: Zhongxing
authors: Z
authors: Zhao
authors: School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, Guangxi, China; Guangxi Key Lab of Petrochemical Resource Processing and Process Intensification Technology, Nanning 530004, Guangxi, China. Electronic address: liaodk@gxu.edu.cn.
authors: Dankui
authors: D
authors: Liao
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D029941:Fish Proteins / Q000737:chemistry* / Q000302:isolation & purification; D005399:Fishes; D006868:Hydrolysis; D009124:Muscle Proteins / Q000737:chemistry* / Q000302:isolation & purification; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification; D011492:Protein Hydrolysates / Q000737:chemistry*; D013381:Subtilisins / Q000737:chemistry
chemical_list: D000975:Antioxidants; D029941:Fish Proteins; D009124:Muscle Proteins; D010455:Peptides; D011492:Protein Hydrolysates; D013381:Subtilisins
keywords: Antioxidative peptide; Characterization; Enzymatic hydrolysis; Purification; Round scad (Decapterus maruadsi)