title: [Optimization of L-phenylalanine-ammonia-lyase lyophilization].
abstract: The influence of freezing-thawing on the activity of recombinant L-phenylalanine-ammonia-lyase has been investigated. Conditions of liophilization of recombinant L-phenylalanine-ammonia-lyase at which enzyme loses about 10% of activity in a single freeze-thaw cycle are chosen. Dependence of cryoscopic temperature of enzyme from concentration of protein in a preparation is studied. The optimum temperature of freezing of enzymatic preparation L-phenylalanine-ammonia-lyase for sublimatic drying is chosen.
authors: O O
authors: OO
authors: Babich
authors: A Iu
authors: AIu
authors: Prosekov
mesh_terms: D001487:Basidiomycota / Q000235:genetics; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics; D005612:Freeze Drying; D005800:Genes, Fungal; D010650:Phenylalanine Ammonia-Lyase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D015202:Protein Engineering / Q000379:methods*
chemical_list: D010650:Phenylalanine Ammonia-Lyase