title: Time-insensitive fluorescent sensor for human serum albumin and its unusual red shift.
abstract: The concentration of human serum albumin (HSA) indicates the health state of individuals and is routinely measured by UV spectroscopy with bromocresol. However, this method tends to overestimate HSA, and more critically, depends highly on the timing, in seconds, of the measurements. Here, we report an analog of 2',7'-dichlorofluorescein that can be used as a fluorescent sensor to quantify HSA in human sera. The accuracy of this new method proved superior to that of bromocresol when an international standard serum sample was analyzed. This method is more convenient than the bromocresol method because it allows for fluorescence measurements during a >15 min period. Colorimetric analysis was also performed to further investigate the effects of the binding of the sensor to HSA. These spectroscopic studies suggest that absorption and emission changes upon HSA binding may be due to the dehydration of the dye and/or stabilization of the tritylic cation species.
authors: Department of Chemistry, University of Pittsburgh , 219 Parkman Avenue, Pittsburgh, Pennsylvania 15260, United States.
authors: Sara E
authors: SE
authors: Smith
authors: Jessica M
authors: JM
authors: Williams
authors: Shin
authors: S
authors: Ando
authors: Kazunori
authors: K
authors: Koide
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D012709:Serum Albumin / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005456:Fluorescent Dyes; D012709:Serum Albumin