title: Low concentration of serum helps to maintain the characteristics of NSCs/NPCs on alkali-treated PHBHHx film in vitro.
abstract: OBJECTIVE
  Efforts have been made by tissue engineers to create a permissive environment for neural regeneration, and to enhance the efficiency of neural stem cell (NSC) transplantation. However, to acquire sufficient number of seed cells on the material appears to be the main obstacle to constructing functional transplantable NSC-biomaterial complexes. A culture system has been optimized in the current study to maintain the specific characteristics of NSCs/neural progenitor cells (NPCs) on the material and achieve sustaining increased multipotent seed cells.
  
  
  METHODS
  The PHBHHx film was selected as biomaterial and the surface was firstly modified with NaOH treatment. The NSCs/NPCs isolated from the cerebral cortex of rat embryos were cultured on the treated PHBHHx films in growth medium containing 1%, 5%, and 10% fetal bovine serum (FBS). Then the attachment, survival, proliferation, and differentiation of NSCs/NPCs were assessed.
  
  
  RESULTS
  NaOH treatment significantly increased the hydrophilicity of PHBHHx and enhanced NSCs/NPCs attachment. On the treated PHBHHx film, NSCs/NPCs survived well and actively proliferated in the medium containing 1% FBS. After 7-14 days in culture, approximately two-thirds of cells remained as nestin and Sox2 positive NSCs/NPCs. However, in the medium containing 5% and 10% FBS, NSCs/NPCs proliferation was reduced and differentiation, particularly glial differentiation was significantly promoted.
  
  
  CONCLUSIONS
  Growth medium containing low concentration of FBS is favorable for maintaining the characteristics, in terms of self-renewal and multiple differentiation, of NSCs/NPCs on NaOH-treated PHBHHx films. This could be a useful method to construct functional transplantable NSCs/NPCs-biomaterial complex.
authors: Hai-xia
authors: HX
authors: Lu
authors: Zhi-qian
authors: ZQ
authors: Yang
authors: Qian
authors: Q
authors: Jiao
authors: Yuan-yuan
authors: YY
authors: Wang
authors: Li
authors: L
authors: Wang
authors: Peng-bo
authors: PB
authors: Yang
authors: Xin-lin
authors: XL
authors: Chen
authors: Peng-bo
authors: PB
authors: Zhang
authors: Peng
authors: P
authors: Wang
authors: Ming-xia
authors: MX
authors: Chen
authors: Xiao-yun
authors: XY
authors: Lu
authors: Yong
authors: Y
authors: Liu
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002208:Caproates / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D002470:Cell Survival; D002540:Cerebral Cortex / Q000166:cytology; D053595:Embryonic Stem Cells / Q000166:cytology; D058953:Neural Stem Cells / Q000166:cytology*; D051381:Rats; D012710:Serum Albumin, Bovine; D012972:Sodium Hydroxide; D013499:Surface Properties; D023822:Tissue Engineering; D000094122:Polyhydroxybutyrates
chemical_list: D020155:3-Hydroxybutyric Acid; D001672:Biocompatible Materials; D002208:Caproates; D012710:Serum Albumin, Bovine; D012972:Sodium Hydroxide; C115940:poly(3-hydroxybutyrate-co-3-hydroxyhexanoate); D000094122:Polyhydroxybutyrates
keywords: Multiple differentiation,; NSCs/NPCs,; PHBHHx,; Self-renewal,; Serum