title: Role of phosphatidylinositol 3-kinase (PI3K) and Akt1 kinase in porcine reproductive and respiratory syndrome virus (PRRSV) replication.
abstract: We have previously reported that inhibition of phosphatidylinositol 3-kinase (PI3K) reduces porcine reproductive and respiratory syndrome (PRRSV) replication. Here, we further investigate the mechanism by which PI3K inhibition affects virus replication and the role of Akt1 kinase in virus replication. We found that PI3K inhibition reduced viral gene transcription by approximately 1.5-fold. Accordingly, viral protein synthesis was significantly reduced by PI3K inhibition. Interestingly, cells overexpressing the dominant negative mutant Akt1 exhibited a significant reduction in viral gene transcription compared to cells overexpressing the constitutively active Akt1. Viral protein synthesis was also enhanced in cells overexpressing the constitutively active Akt1. Overall, our data show that both PI3K and Akt1 play a role in viral gene expression, leading to an increase in virus replication.
authors: Department of Biology and Microbiology, South Dakota State University, Brookings, SD, 57007, USA, xiuqing.wang@sdstate.edu.
authors: Xiuqing
authors: X
authors: Wang
authors: Hanmo
authors: H
authors: Zhang
authors: Alex M
authors: AM
authors: Abel
authors: Alan J
authors: AJ
authors: Young
authors: Liji
authors: L
authors: Xie
authors: Zhixun
authors: Z
authors: Xie
mesh_terms: D000818:Animals; D004261:DNA Replication; D015967:Gene Expression Regulation, Viral; D058539:Phosphatidylinositol 3-Kinase / Q000235:genetics / Q000378:metabolism*; D019318:Porcine Reproductive and Respiratory Syndrome / Q000201:enzymology* / Q000235:genetics / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism*; D013552:Swine; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication
chemical_list: D014764:Viral Proteins; D058539:Phosphatidylinositol 3-Kinase; D051057:Proto-Oncogene Proteins c-akt