title: Does vestibular end-organ function recover after gentamicin-induced trauma in Guinea pigs?
abstract: Until 1993 it was commonly accepted that regeneration of vestibular hair cells was not possible in mammals. Two histological studies then showed structural evidence for spontaneous regeneration of vestibular hair cells after gentamicin treatment. There is less evidence for functional recovery going along with this regenerative process; in other words, do regenerated hair cells function adequately? This study aims to address this question, and in general evaluates whether spontaneous functional recovery may occur, in the short or long term, in mammals after ototoxic insult. Guinea pigs were treated with gentamicin for 10 consecutive days at a daily dose of 125 mg/kg body weight. Survival times varied from 1 day to 16 weeks. Vestibular short-latency evoked potentials (VsEPs) to linear acceleration pulses were recorded longitudinally to assess otolith function. After the final functional measurements we performed immunofluorescence histology for hair cell counts. Auditory brainstem responses (ABRs) to click stimuli were recorded to assess cochlear function. As intended, gentamicin treatment resulted in significant loss of utricular hair cells and accompanying declines in VsEPs. Hair cell counts 8 or 16 weeks after treatment did not significantly differ from counts after shorter survival periods. Maximal functional loss was achieved 1-4 weeks after treatment. After this period, only 2 animals showed recovery of VsEP amplitude - all other animals did not reveal signs of regeneration or recovery. In contrast, after initial ABR threshold shifts there was a small but significant recovery. We conclude that spontaneous recovery of otolith function, in contrast to cochlear function, is very limited in guinea pigs. These results support the concept of intratympanic gentamicin treatment where gentamicin is used for chemoablation of the vestibular sensory epithelia.
authors: Department of Otorhinolaryngology - Head and Neck Surgery, Brain Center Rudolf Magnus, University Medical Center Utrecht, Utrecht, The Netherlands.
authors: Hendrik G
authors: HG
authors: Bremer
authors: Huib
authors: H
authors: Versnel
authors: Ferry G J
authors: FG
authors: Hendriksen
authors: Vedat
authors: V
authors: Topsakal
authors: Wilko
authors: W
authors: Grolman
authors: Sjaak F L
authors: SF
authors: Klis
mesh_terms: D000818:Animals; D016057:Evoked Potentials, Auditory, Brain Stem / Q000187:drug effects / Q000502:physiology*; D005260:Female; D005839:Gentamicins / Q000633:toxicity*; D006168:Guinea Pigs; D018069:Hair Cells, Vestibular / Q000187:drug effects / Q000502:physiology*; D020127:Recovery of Function / Q000187:drug effects / Q000502:physiology*; D012038:Regeneration / Q000502:physiology*; D014722:Vestibule, Labyrinth / Q000187:drug effects / Q000502:physiology*
chemical_list: D005839:Gentamicins