title: Development of a live attenuated vaccine candidate against duck Tembusu viral disease.
abstract: Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that is causing massive economic loss in the Chinese duck industry. To obtain a live vaccine candidate against the disease, the DTMUV isolate FX2010 was passaged serially in chicken embryo fibroblasts (CEFs). Characterization of FX2010-180P revealed that it was unable to replicate efficiently in chicken embryonated eggs, nor intranasally infect mice or shelducks at high doses of 5.5log10 tissue culture infectious doses (TCID50). FX2010-180P did not induce clinical symptoms, or pathological lesions in ducks at a dose of 5.5log10TCID50. The attenuation of FX2010-180P was due to 19 amino acid changes and 15 synonymous mutations. Importantly, FX2010-180P elicited good immune responses in ducks inoculated at low doses (3.5log10TCID50) and provided complete protection against challenge with a virulent strain. These results indicate that FX2010-180P is a promising candidate live vaccine for prevention of duck Tembusu viral disease.
authors: Department of avian infectious disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors: Guoxin
authors: G
authors: Li
authors: Department of avian infectious disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors: Xuyuan
authors: X
authors: Gao
authors: Department of avian infectious disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors: Yali
authors: Y
authors: Xiao
authors: Department of avian infectious disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors: Shaoqiong
authors: S
authors: Liu
authors: Department of avian infectious disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors: Shan
authors: S
authors: Peng
authors: Department of avian infectious disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors: Xuesong
authors: X
authors: Li
authors: Department of avian infectious disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors: Ying
authors: Y
authors: Shi
authors: Department of avian infectious disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors: Yuee
authors: Y
authors: Zhang
authors: Department of avian infectious disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors: Lei
authors: L
authors: Yu
authors: Department of avian infectious disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors: Xiaogang
authors: X
authors: Wu
authors: Department of avian infectious disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors: Pixi
authors: P
authors: Yan
authors: Department of avian infectious disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors: Liping
authors: L
authors: Yan
authors: Department of avian infectious disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors: Qiaoyang
authors: Q
authors: Teng
authors: Department of avian infectious disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China. Electronic address: gztong@shvri.ac.cn.
authors: Guangzhi
authors: G
authors: Tong
authors: Department of avian infectious disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China. Electronic address: lizejun@shvri.ac.cn.
authors: Zejun
authors: Z
authors: Li
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002642:Chick Embryo; D004372:Ducks; D005260:Female; D005416:Flavivirus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity; D018177:Flavivirus Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014774:Virulence
chemical_list: D000914:Antibodies, Viral; D014613:Vaccines, Attenuated; D014765:Viral Vaccines
keywords: Attenuation; Duck Tembusu virus; Flavivirus