title: Characterization of microalgae-bacteria consortium cultured in landfill leachate for carbon fixation and lipid production.
abstract: The characteristics of cultivating high-density microalgae-bacteria consortium with landfill leachate was tested in this study. Landfill leachate was collected from Laogang landfill operated for over 10 years in Shanghai, China. The maximum biomass concentration of 1.58g L(-1) and chlorophyll a level of 22mg L(-1) were obtained in 10% leachate spike ratio. Meanwhile, up to 90% of the total nitrogen in landfill leachate was removed in culture with 10% leachate spike ratio with a total nitrogen concentration of 221.6mg L(-1). The fluorescence peak of humic-like organic matters red shifted to longer wavelengths by the end of culture, indicating that microalgae-bacteria consortium was effective for treating landfill leachate contaminants. Furthermore, with the leachate spike ratio of 10%, the maximum lipid productivity and carbon fixation were 24.1 and 65.8mg L(-1)d(-1), respectively. Results of this research provide valuable information for optimizing microalgae culture in landfill leachate.
authors: State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092, China.
authors: Xin
authors: X
authors: Zhao
authors: Department of Agricultural and Biological Engineering, University of Illinois at Urbana-Champaign 1304 W. Pennsylvania Ave, AESB, Urbana, IL, United States.
authors: Yan
authors: Y
authors: Zhou
authors: State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092, China.
authors: Sheng
authors: S
authors: Huang
authors: State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092, China.
authors: Duanyang
authors: D
authors: Qiu
authors: Department of Agricultural and Biological Engineering, University of Illinois at Urbana-Champaign 1304 W. Pennsylvania Ave, AESB, Urbana, IL, United States.
authors: Lance
authors: L
authors: Schideman
authors: State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092, China. Electronic address: xlchai@tongji.edu.cn.
authors: Xiaoli
authors: X
authors: Chai
authors: State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092, China.
authors: Youcai
authors: Y
authors: Zhao
mesh_terms: D000641:Ammonia / Q000032:analysis; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development*; D061249:Batch Cell Culture Techniques; D057486:Carbon Cycle / Q000187:drug effects*; D002681:China; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000187:drug effects / Q000254:growth & development*; D059013:Microbial Consortia; D009584:Nitrogen / Q000032:analysis / Q000302:isolation & purification; D009930:Organic Chemicals / Q000032:analysis; D010758:Phosphorus / Q000302:isolation & purification; D013050:Spectrometry, Fluorescence; D062065:Wastewater / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000641:Ammonia; D008055:Lipids; D009584:Nitrogen; D009930:Organic Chemicals; D010758:Phosphorus; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Biofuel production; Carbon fixation; Landfill leachate; Microalgae; Microalgae-bacteria consortium