title: Structural elucidation and in vitro antitumor activity of a novel oligosaccharide from Bombyx batryticatus.
abstract: An oligosaccharide BBPW-2 was isolated and purified from Bombyx batryticatus, its molecular weight was 2.0 × 10(3)Da, and its structure was elucidated by compositional, methylation and NMR analysis. Our results showed that BBPW-2 consisted of β-D-(1 → 2,6)-glucopyranose and β-D-(1 → 2,6)-mannosyl units serving as the backbone, α-D-(1 → 2)-galactopyranose and α-D-(1 → 3)-mannosyl units as branches, and α-D-Manp and β-D-Glcp as terminals. The in vitro inhibitory activity of BBPW-2 was measured using MTT and and crystal violet assays, which suggested that BBPW-2 had direct cytotoxic effects on the cancer cell lines HeLa and HepG2 (particularly HeLa cells), and had a long-term antiproliferative effect on MCF-7 cells, respectively. Apoptosis and cellcycle analysis of HeLa cells showed that BBPW-2 induced cellcycle disruption in the G0/G1 and G2/M phases accompanied by an impressive increment of early apoptotic cells and late apoptotic and necrotic cells. These results suggest that BBPW-2 could be a potential chemotherapeutic drug and its antitumor effects deserve further study.
authors: College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
authors: Xue
authors: X
authors: Jiang
authors: Institute of Horticulture, Zhejiang Academy of Agricultural Science, Hangzhou, Zhejiang 310021, China.
authors: Zuofa
authors: Z
authors: Zhang
authors: College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
authors: Yajie
authors: Y
authors: Chen
authors: College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
authors: Zhenteng
authors: Z
authors: Cui
authors: College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China. Electronic address: slgsilk@zju.edu.cn.
authors: Liangen
authors: L
authors: Shi
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D012831:Bombyx / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D061986:MCF-7 Cells; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D009844:Oligosaccharides
keywords: Antitumor activity; Bombyx batryticatus; Oligosaccharide; Structural elucidation