title: Association of azoospermia factor region deletions in infertile male subjects among Malaysians.
abstract: Azoospermia factor region (AZF) deletions (AZFa, AZFb, AZFc and AZFd) in the Y chromosome were analysed in male infertility subjects in various populations with conflicting results. This study comprised of 54 infertile males and 63 fertile controls, and the frequency of AZFa, AZFb, AZFc and AZFd deletions were determined using conventional polymerase chain reaction (PCR) as well as real-time PCR-high resolution melting analysis-based methods. The results of this study showed that, three of 54 cases (5.55%) had AZF (a, b and c) deletions (two had AZFc and one had AZFa deletions). Four cases were found to have AZFd deletions (7.4%) with two of them being associated with AZFc deletions (P = 0.028). The frequency of AZF (a, b and c) deletions in Malaysian infertile male subjects was found to be comparable with other populations. AZFd deletions were found to be significant (P < 0.05) in male infertility and it may be associated with other types of AZF deletions.
authors: Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Malaysia.
authors: A A
authors: AA
authors: Hussein
authors: R
authors: R
authors: Vasudevan
authors: I
authors: I
authors: Patimah
authors: N
authors: N
authors: Prashant
authors: F A
authors: FA
authors: Nora
mesh_terms: D000328:Adult; D053713:Azoospermia / Q000235:genetics*; D016022:Case-Control Studies; D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y / Q000235:genetics*; D005820:Genetic Testing; D005838:Genotype; D006801:Humans; D007248:Infertility, Male / Q000453:epidemiology / Q000208:ethnology* / Q000235:genetics*; D008296:Malaysia / Q000453:epidemiology; D008297:Male; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods; D012307:Risk Factors
keywords: Azoospermia factor region; high resolution melt; male infertility