title: Macrophage migration inhibitory factor: association of -794 CATT5-8 and -173 G>C polymorphisms with TNF-α in systemic lupus erythematosus.
abstract: Macrophage migration inhibitory factor (MIF) is an upstream immunoregulatory cytokine associated with the pathogenesis of autoimmune inflammatory diseases. There is evidence that MIF functions in a positive feedback loop with TNF-α that could perpetuate the inflammatory process in systemic lupus erythematosus (SLE). In this case-control study we investigated whether commonly occurring functional MIF polymorphisms are associated with SLE as well as with MIF and TNF-α serum levels in a Mexican-Mestizo population. Genotyping of the -794 CATT5-8 (rs5844572) and -173 G>C (rs755622) MIF polymorphisms was performed by PCR and PCR-RFLP, respectively in 186 SLE patients and 200 healthy subjects. MIF and TNF-α serum levels were determined by ELISA. A significant increase of MIF and TNF-α levels was found in SLE patients. According to a genetic model, we found a significant association of genotypes carrying the -794 CATT7 and -173(∗)C risk alleles with susceptibility to SLE and with a significant increase of TNF-α. In conclusion, MIF gene polymorphisms are associated with SLE susceptibility and with an increase of TNF-α serum levels in a Mexican-Mestizo population.
authors: Instituto de Investigación en Ciencias Biomédicas/Programa de Doctorado en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
authors: U
authors: U
authors: De la Cruz-Mosso
authors: Department of Medicine/Section of Rheumatology, Yale University School of Medicine, New Haven, USA.
authors: R
authors: R
authors: Bucala
authors: Instituto de Investigación en Ciencias Biomédicas/Programa de Doctorado en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
authors: C A
authors: CA
authors: Palafox-Sánchez
authors: Unidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo, Guerrero, Mexico.
authors: I
authors: I
authors: Parra-Rojas
authors: Instituto de Investigación en Ciencias Biomédicas/Programa de Doctorado en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
authors: J R
authors: JR
authors: Padilla-Gutiérrez
authors: Instituto de Investigación en Ciencias Biomédicas/Programa de Doctorado en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
authors: A L
authors: AL
authors: Pereira-Suárez
authors: Instituto de Genética Molecular, Centro Universitario de la Ciénega, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
authors: H
authors: H
authors: Rangel-Villalobos
authors: Instituto de Investigación en Ciencias Biomédicas/Programa de Doctorado en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
authors: M
authors: M
authors: Vázquez-Villamar
authors: Instituto de Investigación en Ciencias Biomédicas/Programa de Doctorado en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
authors: L I
authors: LI
authors: Angel-Chávez
authors: Instituto de Investigación en Ciencias Biomédicas/Programa de Doctorado en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico. Electronic address: biologiamolecular@hotmail.com.
authors: J F
authors: JF
authors: Muñoz-Valle
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000235:genetics*; D008263:Macrophage Migration-Inhibitory Factors / Q000097:blood / Q000235:genetics*; D008297:Male; D008800:Mexico; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000235:genetics; D055815:Young Adult
chemical_list: D008263:Macrophage Migration-Inhibitory Factors; D014409:Tumor Necrosis Factor-alpha