title: Effects of dietary selenium on histopathological changes and T cells of spleen in broilers exposed to aflatoxin B1.
abstract: Aflatoxin B1 (AFB1), which causes hepatocellular carcinoma and immune-suppression, is commonly found in feedstuffs. To evaluate the ability of selenium (Se) to counteract the deleterious effects of AFB1, two hundred 1-day-old male avian broilers, divided into five groups, were fed with basal diet (control group), 0.3 mg/kg AFB1 (AFB1 group), 0.3 mg/kg AFB1+0.2 mg/kg Se (+Se group I), 0.3 mg/kg AFB1+0.4 mg/kg Se (+Se group II) and 0.3 mg/kg AFB1+0.6 mg/kg Se (+Se group III), respectively. Compared with control group, the relative weight of spleen in the AFB1 group was decreased at 21 days of age. The relative weight of spleen in the three +Se groups was higher than that in the AFB1 group. By pathological observation, the major spleen lesions included congestion in red pulp and vacuoles appeared in the lymphatic nodules and periarterial lymphatic sheath in the AFB1 group. In +Se groups II and III, the incidence of major splenic lesions was decreased. The percentages of CD3+, CD3+CD4+ and CD3+CD8+ T cells in the AFB1 group were lower than those in control group from 7 to 21 days of age, while there was a marked increase in the three +Se groups compared to the AFB1 group. The results indicated that sodium selenite could improve the cellular immune function impaired by AFB1 through increasing the relative weight of spleen and percentages of splenic T cell subsets, and alleviating histopathological spleen damage.
authors: Department of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China. ckj930@126.com.
authors: Kejie
authors: K
authors: Chen
authors: Department of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China. pengxi197313@163.com.
authors: Xi
authors: X
authors: Peng
authors: Department of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China. fangjing4109@163.com.
authors: Jing
authors: J
authors: Fang
authors: Department of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China. cui580420@sicau.edu.cn.
authors: Hengmin
authors: H
authors: Cui
authors: Department of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China. zzcjl@126.com.
authors: Zhicai
authors: Z
authors: Zuo
authors: Department of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China. dengjl213@126.com.
authors: Junliang
authors: J
authors: Deng
authors: Department of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China. chzhli75@163.com.
authors: Zhengli
authors: Z
authors: Chen
authors: Department of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China. gengyisicau@126.com.
authors: Yi
authors: Y
authors: Geng
authors: Department of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China. nwm_mm2004@163.com.
authors: Weimin
authors: W
authors: Lai
authors: Department of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China. tangyimingtt@163.com.
authors: Li
authors: L
authors: Tang
authors: Department of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China. magician-rookies@hotmail.com.
authors: Qingqiu
authors: Q
authors: Yang
mesh_terms: D016604:Aflatoxin B1 / Q000633:toxicity*; D000818:Animals; D002645:Chickens; D019587:Dietary Supplements; D008297:Male; D009929:Organ Size / Q000187:drug effects; D012643:Selenium / Q000494:pharmacology* / Q000627:therapeutic use; D018038:Sodium Selenite / Q000494:pharmacology*; D013154:Spleen / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D013158:Splenic Diseases / Q000188:drug therapy / Q000473:pathology; D016176:T-Lymphocyte Subsets / Q000187:drug effects* / Q000473:pathology
chemical_list: D016604:Aflatoxin B1; D012643:Selenium; D018038:Sodium Selenite