title: LncRNA profile study reveals a three-lncRNA signature associated with the survival of patients with oesophageal squamous cell carcinoma.
abstract: BACKGROUND
  Oesophageal cancer is one of the most deadly forms of cancer worldwide. Long non-coding RNAs (lncRNAs) are often found to have important regulatory roles.
  
  
  OBJECTIVE
  To assess the lncRNA expression profile of oesophageal squamous cell carcinoma (OSCC) and identify prognosis-related lncRNAs.
  
  
  METHODS
  LncRNA expression profiles were studied by microarray in paired tumour and normal tissues from 119 patients with OSCC and validated by qRT-PCR. The 119 patients were divided randomly into training (n=60) and test (n=59) groups. A prognostic signature was developed from the training group using a random Forest supervised classification algorithm and a nearest shrunken centroid algorithm, then validated in a test group and further, in an independent cohort (n=60). The independence of the signature in survival prediction was evaluated by multivariable Cox regression analysis.
  
  
  RESULTS
  LncRNAs showed significantly altered expression in OSCC tissues. From the training group, we identified a three-lncRNA signature (including the lncRNAs ENST00000435885.1, XLOC_013014 and ENST00000547963.1) which classified the patients into two groups with significantly different overall survival (median survival 19.2 months vs >60 months, p<0.0001). The signature was applied to the test group (median survival 21.5 months vs >60 months, p=0.0030) and independent cohort (median survival 25.8 months vs >48 months, p=0.0187) and showed similar prognostic values in both. Multivariable Cox regression analysis showed that the signature was an independent prognostic factor for patients with OSCC. Stratified analysis suggested that the signature was prognostic within clinical stages.
  
  
  CONCLUSIONS
  Our results suggest that the three-lncRNA signature is a new biomarker for the prognosis of patients with OSCC, enabling more accurate prediction of survival.
authors: ! Department of Thoracic Surgery, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, The People's Republic of China.
authors: Jiagen
authors: J
authors: Li
authors: ! Department of Thoracic Surgery, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, The People's Republic of China.
authors: Zhaoli
authors: Z
authors: Chen
authors: Bioinformatics Laboratory and Laboratory of Noncoding RNA, Institute of Biophysics, Chinese Academy of Sciences, Beijing, The People's Republic of China.
authors: Liqing
authors: L
authors: Tian
authors: ! Department of Thoracic Surgery, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, The People's Republic of China.
authors: Chengcheng
authors: C
authors: Zhou
authors: ! Department of Thoracic Surgery, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, The People's Republic of China.
authors: Max Yifan
authors: MY
authors: He
authors: ! Department of Thoracic Surgery, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, The People's Republic of China.
authors: Yibo
authors: Y
authors: Gao
authors: ! Department of Thoracic Surgery, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, The People's Republic of China.
authors: Suya
authors: S
authors: Wang
authors: ! Department of Thoracic Surgery, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, The People's Republic of China.
authors: Fang
authors: F
authors: Zhou
authors: Department of Pathology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, The People's Republic of China.
authors: Susheng
authors: S
authors: Shi
authors: Department of Pathology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, The People's Republic of China.
authors: Xiaoli
authors: X
authors: Feng
authors: ! Department of Thoracic Surgery, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, The People's Republic of China.
authors: Nan
authors: N
authors: Sun
authors: ! Department of Thoracic Surgery, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, The People's Republic of China.
authors: Ziyuan
authors: Z
authors: Liu
authors: Bioinformatics Laboratory and Laboratory of Noncoding RNA, Institute of Biophysics, Chinese Academy of Sciences, Beijing, The People's Republic of China.
authors: Geir
authors: G
authors: Skogerboe
authors: ! Department of Thoracic Surgery, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, The People's Republic of China.
authors: Jingsi
authors: J
authors: Dong
authors: ! Department of Thoracic Surgery, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, The People's Republic of China.
authors: Ran
authors: R
authors: Yao
authors: ! Department of Thoracic Surgery, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, The People's Republic of China.
authors: Yuda
authors: Y
authors: Zhao
authors: ! Department of Thoracic Surgery, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, The People's Republic of China.
authors: Jian
authors: J
authors: Sun
authors: ! Department of Thoracic Surgery, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, The People's Republic of China.
authors: Baihua
authors: B
authors: Zhang
authors: ! Department of Thoracic Surgery, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, The People's Republic of China.
authors: Yue
authors: Y
authors: Yu
authors: ! Department of Thoracic Surgery, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, The People's Republic of China.
authors: Xuejiao
authors: X
authors: Shi
authors: ! Department of Thoracic Surgery, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, The People's Republic of China.
authors: Mei
authors: M
authors: Luo
authors: ! Department of Thoracic Surgery, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, The People's Republic of China.
authors: Kang
authors: K
authors: Shao
authors: ! Department of Thoracic Surgery, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, The People's Republic of China.
authors: Ning
authors: N
authors: Li
authors: ! Department of Thoracic Surgery, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, The People's Republic of China.
authors: Bin
authors: B
authors: Qiu
authors: ! Department of Thoracic Surgery, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, The People's Republic of China.
authors: Fengwei
authors: F
authors: Tan
authors: Bioinformatics Laboratory and Laboratory of Noncoding RNA, Institute of Biophysics, Chinese Academy of Sciences, Beijing, The People's Republic of China.
authors: Runsheng
authors: R
authors: Chen
authors: ! Department of Thoracic Surgery, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, The People's Republic of China.
authors: Jie
authors: J
authors: He
mesh_terms: D014408:Biomarkers, Tumor / Q000502:physiology; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000401:mortality*; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000401:mortality*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D062085:RNA, Long Noncoding / Q000378:metabolism*; D012372:ROC Curve; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D059467:Transcriptome / Q000502:physiology
chemical_list: D014408:Biomarkers, Tumor; D062085:RNA, Long Noncoding
keywords: Oesophageal Cancer