title: Utilization of alkali-tolerant strains in fermentation of excess sludge.
abstract: This study aimed at exploring a new approach for producing volatile fatty acids (VFAs) from excess sludge (ES). Two representative alkali-tolerant bacteria (HIT-01 and HIT-02) were isolated from ES, and inoculated separately or jointly into ES to investigate their effects on VFAs accumulation, soluble organic compounds concentrations, and bacterial community structure of ES under alkaline anaerobic conditions (pH 10.0). Four fermentation conditions were examined for 20days. Joint-inoculation with the two strains achieved the highest VFAs concentration (3139mg/L). Acetic and propionic acids were the dominant acid species. On the 9th day, all the inoculated ES had the higher concentrations of soluble organic compounds than the un-inoculated control ES. Denaturing gradient gel electrophoresis (DGGE) analysis revealed that these two strains (HIT-01 and HIT-02) became predominant in the bacterial community during alkaline anaerobic fermentation. The results demonstrated that bioaugmentation might be useful for enhancing VFAs accumulation from ES.
authors: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China; Department of Food and Environment Engineering, East University of Heilongjiang, Harbin 150086, China.
authors: Weiguang
authors: W
authors: Jie
authors: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China; Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China. Electronic address: pyz@bjut.edu.cn.
authors: Yongzhen
authors: Y
authors: Peng
authors: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
authors: Nanqi
authors: N
authors: Ren
authors: Department of Civil and Environmental Engineering, University of Connecticut, Storrs, CT 06269, USA.
authors: Baikun
authors: B
authors: Li
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects*; D000468:Alkalies / Q000494:pharmacology*; D064751:Ammonium Compounds / Q000378:metabolism; D001419:Bacteria / Q000302:isolation & purification / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D057919:Biological Oxygen Demand Analysis; D002241:Carbohydrates / Q000032:analysis; D004275:DNA, Ribosomal / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D005232:Fatty Acids, Volatile / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects*; D008969:Molecular Sequence Data; D010758:Phosphorus / Q000378:metabolism; D010802:Phylogeny; D012722:Sewage / Q000382:microbiology*; D012995:Solubility
chemical_list: D000468:Alkalies; D064751:Ammonium Compounds; D001426:Bacterial Proteins; D002241:Carbohydrates; D004275:DNA, Ribosomal; D005232:Fatty Acids, Volatile; D012722:Sewage; D010758:Phosphorus
keywords: Alkali-tolerant stains; Excess sludge (ES); Fermentation; Microbial community structure; Volatile fatty acids (VFAs)