title: [Protective effects of hydrogen on renal ischemia/reperfusion injury in rats].
abstract: OBJECTIVE
  To investigate the protective effects of hydrogen on renal ischemic/reperfusion injury and its mechanism in rats.
  
  
  METHODS
  Eighteen adult male Sprague-Dawley rats were used and allocated into sham-operated group (Sham group), ischemic reperfusion group (I/R group) and hydrogen treated group (H2 group) randomly. Ischemic/reperfusion of the left renal pedicles following a right nephrectomy was investigated in I/R group. In the Sham group, the rats were subjected to identical surgical procedure without occlusion of left renal pedicles. In the H2 group, inhalation of the 2.5% hydrogen was initiated 10 min before reperfusion and last for 130 min till the end of the 120-min reperfusion. Plasma level of usea nitrogen (BUN), creatinine (Cr), maiondiaidehyde (MDA), the score of histopathological examination of left kidney were evaluated 24 h after operation in all subjects.
  
  
  RESULTS
  Ischemia-reperfusion injury rat model was established successfully. Inhalation of hydrogen (2.5%) markedly reduced the level of serum BUN, CREA and MDA (P < 0.05). The histopathological outcome in the H2 group was significantly improved, compared to the I/R group (P < 0.05).
  
  
  CONCLUSIONS
  Inhalation of hydrogen could attenuate renal ischemic/reperfusion injury in rats by decreasing the content of MDA.
authors: Department of Anesthesiology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
authors: Kui
authors: K
authors: Zeng
authors: Department of Anesthesiology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
authors: Han
authors: H
authors: Huang
authors: Department of Anesthesiology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
authors: Xiao-Qin
authors: XQ
authors: Jiang
authors: Department of Anesthesiology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
authors: Xiao-Jing
authors: XJ
authors: Chen
authors: Department of Anesthesiology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
authors: Wei
authors: W
authors: Huang
mesh_terms: D000818:Animals; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D006859:Hydrogen / Q000627:therapeutic use*; D007668:Kidney / Q000473:pathology*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009392:Nephrectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000628:therapy*
chemical_list: D008315:Malondialdehyde; D006859:Hydrogen; D003404:Creatinine