title: Expression and roles of As-NUPR1 protein from Artemia sinica during embryo development and in response to salinity stress.
abstract: As-NUPR1, a stress-related protein, plays an important role in post-diapause during embryonic development in the brine shrimp Artemia sinica. In the present study, successful expression of As-NUPR1 from the cDNA sequence isolated from A. sinica was demonstrated using a prokaryotic expression system. The recombinant protein consisted of 132 amino acids with a molecular weight of 15 kDa, and a predicted isoelectric point of 7.17. As-NUPR1 polyclonal antibodies were prepared by immunization of Balb/c mice with purified recombinant As-NUPR1 protein as an antigen, and immunological studies were carried out. Expression of As-NUPR1 during different developmental stages of the embryo and in response to salinity stress was analyzed in A. sinica using Western blots. The experimental results showed that the expression of As-NUPR1 is widely distributed at different developmental stages in A. sinica, and there was no tissue or organ specificity. Expression of As-NUPR1 decreased gradually during the diapause termination stage of embryo development, after which there was a general increase in expression after breaking the shell. In addition, As-NUPR1 expression was highly upregulated under conditions of high salinity. These results suggest that the As-NUPR1 protein is a stress-related protein that plays a role in protecting embryos from high salt damage in different embryonic developmental stages, especially during the post-diapause period.
authors: College of Life Sciences, Liaoning Normal University, No. 1, Liushu South Street, Ganjingzi District, Dalian, 116081, People's Republic of China.
authors: Rui
authors: R
authors: Zhang
authors: Feng
authors: F
authors: Yao
authors: Cheng
authors: C
authors: Cheng
authors: Yifei
authors: Y
authors: Chen
authors: Ying
authors: Y
authors: Lv
authors: Zengrong
authors: Z
authors: Li
authors: Na
authors: N
authors: Zhao
authors: Ting
authors: T
authors: Wang
authors: Wenting
authors: W
authors: Xin
authors: Xiangyang
authors: X
authors: Zou
authors: Lin
authors: L
authors: Hou
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001156:Artemia / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D003001:Cloning, Molecular; D019295:Computational Biology; D018076:DNA, Complementary; D047108:Embryonic Development / Q000235:genetics*; D015870:Gene Expression; D008969:Molecular Sequence Data; D010802:Phylogeny; D021381:Protein Transport; D054712:Salinity; D016415:Sequence Alignment; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D018076:DNA, Complementary