title: Differentiated human stem cells resemble fetal, not adult, β cells.
abstract: Human pluripotent stem cells (hPSCs) have the potential to generate any human cell type, and one widely recognized goal is to make pancreatic β cells. To this end, comparisons between differentiated cell types produced in vitro and their in vivo counterparts are essential to validate hPSC-derived cells. Genome-wide transcriptional analysis of sorted insulin-expressing (INS(+)) cells derived from three independent hPSC lines, human fetal pancreata, and adult human islets points to two major conclusions: (i) Different hPSC lines produce highly similar INS(+) cells and (ii) hPSC-derived INS(+) (hPSC-INS(+)) cells more closely resemble human fetal β cells than adult β cells. This study provides a direct comparison of transcriptional programs between pure hPSC-INS(+) cells and true β cells and provides a catalog of genes whose manipulation may convert hPSC-INS(+) cells into functional β cells.
authors: Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute and Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138.
authors: Sinisa
authors: S
authors: Hrvatin
authors: Charles W
authors: CW
authors: O'Donnell
authors: Francis
authors: F
authors: Deng
authors: Jeffrey R
authors: JR
authors: Millman
authors: Felicia Walton
authors: FW
authors: Pagliuca
authors: Philip
authors: P
authors: DiIorio
authors: Alireza
authors: A
authors: Rezania
authors: David K
authors: DK
authors: Gifford
authors: Douglas A
authors: DA
authors: Melton
mesh_terms: D000328:Adult; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D005333:Fetus / Q000166:cytology / Q000378:metabolism; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D006801:Humans; D050417:Insulin-Secreting Cells / Q000166:cytology* / Q000378:metabolism; D046228:Microarray Analysis; D010179:Pancreas / Q000166:cytology*; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism
keywords: MARIS; beta cells; differentiation; transcriptional profiling