title: Altered miRNA expression profiles are involved in the protective effects of troxerutin against ultraviolet B radiation in normal human dermal fibroblasts.
abstract: The aim of this study was to investigate the mechanisms by which troxerutin protects cells against ultraviolet B (UVB) radiation. First, we demonstrate that pre-treatment with troxerutin protects normal human dermal fibroblasts (nHDFs) against UVB-induced cytotoxicity. As shown by migration assay and DNA repair analysis, troxerutin increased cell migration and DNA repair activity in the nHDFs. Subsequently, we analyzed microRNA (miRNA) expression profiles in the nHDFs. miRNAs are 19- to 24-nucleotide (nt) non-coding RNA molecules that regulate the translation of target genes through RNA interference. In UVB-exposed cells, miRNAs act on crucial functions, such as apoptosis and cellular senescence. miRNA expression is significantly altered during the protective process induced by phytochemicals. Therefore, understanding changes that occur in miRNA expression profiles may help to elucidate the protective mechanisms of troxerutin. We identified 11 miRNAs that were significantly (>2-fold) upregulated and 12 that were significantly downregulated (>2-fold) following treatment of the nHDFs with troxerutin. In addition, we investigated the biological functions of these miRNAs through the prediction of miRNA targets and Gene Ontology analysis of the putative targets. Overall, our findings indicate that pre-treatment with troxerutin increases the viability of UVB-exposed nHDFs through the alteration of the miRNA expression profiles.
authors: Korea Institute for Skin and Clinical Sciences and Molecular-Targeted Drug Research Center, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Hwa Jun
authors: HJ
authors: Cha
authors: Songpa R&D Center, Coreana Cosmetics Co., Ltd., Cheonan, Chungcheongnam-do 330-833, Republic of Korea.
authors: Kwang Sik
authors: KS
authors: Lee
authors: Songpa R&D Center, Coreana Cosmetics Co., Ltd., Cheonan, Chungcheongnam-do 330-833, Republic of Korea.
authors: Ghang Tai
authors: GT
authors: Lee
authors: Songpa R&D Center, Coreana Cosmetics Co., Ltd., Cheonan, Chungcheongnam-do 330-833, Republic of Korea.
authors: Kun Kook
authors: KK
authors: Lee
authors: College of Pharmacy and Medical Research Center, Chungbuk National University, Cheongju, Chungcheongbuk‑do 361-763, Republic of Korea.
authors: Jin Tae
authors: JT
authors: Hong
authors: Department of Cosmetology, Kyung-In Women's College, Incheon 407-740, Republic of Korea.
authors: Sung Nae
authors: SN
authors: Lee
authors: School of Art, Kyungbok University, Namyangju, Gyeonggi-do 472-948, Republic of Korea.
authors: Hyun Hee
authors: HH
authors: Jang
authors: Laboratory of Molecular Oncology, Cheil General Hospital and Women's Healthcare Center, Kwandong University College of Medicine, Seoul 100‑380, Republic of Korea.
authors: Jae Ho
authors: JH
authors: Lee
authors: Laboratory of Functional Genomics, Korea Institute of Radiological and Medical Sciences, Seoul 139-706, Republic of Korea.
authors: In-Chul
authors: IC
authors: Park
authors: Department of Dermatology, Konkuk University School of Medicine, Seoul 143-701, Republic of Korea.
authors: Yu Ri
authors: YR
authors: Kim
authors: Department of Dermatology, Konkuk University School of Medicine, Seoul 143-701, Republic of Korea.
authors: Kyu Joong
authors: KJ
authors: Ahn
authors: Korea Institute for Skin and Clinical Sciences and Molecular-Targeted Drug Research Center, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Seung Bin
authors: SB
authors: Kwon
authors: Korea Institute for Skin and Clinical Sciences and Molecular-Targeted Drug Research Center, Konkuk University, Seoul 143-701, Republic of Korea.
authors: In-Sook
authors: IS
authors: An
authors: Korea Institute for Skin and Clinical Sciences and Molecular-Targeted Drug Research Center, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Sungkwan
authors: S
authors: An
authors: Korea Institute for Skin and Clinical Sciences and Molecular-Targeted Drug Research Center, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Seunghee
authors: S
authors: Bae
mesh_terms: D016923:Cell Death / Q000187:drug effects / Q000528:radiation effects; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D019610:Cytoprotection / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D004260:DNA Repair / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D020405:Dermis / Q000166:cytology*; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D020869:Gene Expression Profiling; D063990:Gene Ontology; D006801:Humans; D006896:Hydroxyethylrutoside / Q000031:analogs & derivatives* / Q000494:pharmacology; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology; D014466:Ultraviolet Rays; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects
chemical_list: D006896:Hydroxyethylrutoside; D035683:MicroRNAs; D020011:Protective Agents; C005865:troxerutin