title: Effect of RNA interference therapy on the mice eosinophils CCR3 gene and granule protein in the murine model of allergic rhinitis.
abstract: OBJECTIVE
  To observe the clinical manifestations of allergic rhinitis mice and the expression changes of the eosinophils CCR3 and the granule protein mRNA in the bone marrow, peripheral blood and nasal lavage fluid.
  
  
  METHODS
  Twenty-four BALB/c mice were randomly divided into the control group, PBS therapy group, siRNA therapy group and the CCR3 siRNA therapy group (n=6). Allergic rhinitis model were sensitized and stimulated by ovalbunfin, and CCR3 siRNA therapy group were administered with CCR3 transnasally before stimulated. The levels of the eosinophils CCR3, MBP, ECP and EPO in bone marrow, peripheral blood and nasal lavage fluid were detected by RT-PCR.
  
  
  RESULTS
  Compared to the control group and CCR3 siRNA therapy group, the nasal mucosa of the PBS therapy group and siRNA therapy group developed epithalaxy, goblet cells hyperplasia, squamous epithelium metaplasia, epithelium necrosis, lamina propria and submucosa gland hyperplasia, vasodilatation, tissue edema, and the characterized eosinophil infiltration. RT-PCR indicated that the CCR3 mRNA, MBP, ECP and EPO expression in bone marrow, peripheral blood and nasal lavage fluid of the CCR3 siRNA therapy group was lower than the PBS therapy group and siRNA therapy group (P<0.05).
  
  
  CONCLUSIONS
  The RNA interference therapy to CCR3 by local administration pernasal can suppress the process of the development, migration and invasion of the allergic rhinitis eosinophil, thus can reduce the effect of eosinophils and then reduce the inflammation effect of the allergic rhinitis. It may be a new treatment for respiratory tract allergic inflammation.
authors: Department of Otorhinolaryngology Head and Neck Surgery, the Second Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors: Xin-Hua
authors: XH
authors: Zhu
authors: Department of Head and Neck Surgery, the Tumor Hospital of Jiangxi Province, Nanchang 330006, China.
authors: Bing
authors: B
authors: Liao
authors: Department of Otorhinolaryngology Head and Neck Surgery, the Second Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors: Ke
authors: K
authors: Liu
authors: Department of Otorhinolaryngology Head and Neck Surgery, the Second Affiliated Hospital of Nanchang University, Nanchang 330006, China. Electronic address: liuyuehuiclark@21cn.com.
authors: Yue-Hui
authors: YH
authors: Liu
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D001853:Bone Marrow / Q000737:chemistry; D004195:Disease Models, Animal; D047091:Eosinophil Granule Proteins / Q000235:genetics / Q000378:metabolism*; D004804:Eosinophils / Q000378:metabolism / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009297:Nasal Mucosa / Q000737:chemistry / Q000166:cytology; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics; D011897:Random Allocation; D054397:Receptors, CCR3 / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*; D065631:Rhinitis, Allergic; D012221:Rhinitis, Allergic, Perennial / Q000235:genetics / Q000628:therapy*
chemical_list: C515351:Ccr3 protein, mouse; D047091:Eosinophil Granule Proteins; D034741:RNA, Small Interfering; D054397:Receptors, CCR3
keywords: Allergic rhinitis; CCR3; Eosinophil; RNA interfering