title: Mueller matrix three-dimensional directional imaging of collagen fibers.
abstract: A method for measuring three-dimensional (3-D) direction images of collagen fibers in biological tissue is presented. Images of the 3-D directions are derived from the measured transmission Mueller matrix images (MMIs), acquired at different incidence angles, by taking advantage of the form birefringence of the collagen fibers. The MMIs are decomposed using the recently developed differential decomposition, which is more suited to biological tissue samples than the common polar decomposition method. Validation of the 3-D direction images was performed by comparing them with images from second-harmonic generation microscopy. The comparison found a good agreement between the two methods. It is envisaged that 3-D directional imaging could become a useful tool for understanding the collagen framework for fibers smaller than the diffraction limit.
authors: Pål Gunnar
authors: PG
authors: Ellingsen
authors: Lars Martin Sandvik
authors: LM
authors: Aas
authors: Vegard Stenhjem
authors: VS
authors: Hagen
authors: Rajesh
authors: R
authors: Kumar
authors: Magnus Borstad
authors: MB
authors: Lilledahl
authors: Morten
authors: M
authors: Kildemo
mesh_terms: D000162:Acoustics; D000818:Animals; D002645:Chickens; D003094:Collagen / Q000648:ultrastructure*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D015203:Reproducibility of Results; D013710:Tendons / Q000737:chemistry / Q000648:ultrastructure
chemical_list: D003094:Collagen