title: Validation of a HPLC method for determination of hydroxymethylfurfural in crude palm oil.
abstract: For the first time 5-hydroxymethyl-2-furaldehyde (HMF) was separated from crude palm oil (CPO), and its authenticity was determined using an RP-HPLC method. Separation was accomplished with isocratic elution of a mobile phase comprising water and methanol (92:8 v/v) on a Purospher Star RP-18e column (250mm×4.6mm, 5.0μm). The flow rate was adjusted to 1ml/min and detection was performed at 284nm. The method was validated, and results obtained exhibit a good recovery (95.58% to 98.39%). Assessment of precision showed that the relative standard deviations (RSD%) of retention times and peak areas of spiked samples were less than 0.59% and 2.66%, respectively. Further, the limit of detection (LOD) and LOQ were 0.02, 0.05mg/kg, respectively, and the response was linear across the applied ranges. The crude palm oil samples analysed exhibited HMF content less than 2.27mg/kg.
authors: Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. Electronic address: abdulazis@putra.upm.edu.my.
authors: Abdul Azis
authors: AA
authors: Ariffin
authors: Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors: H M
authors: HM
authors: Ghazali
authors: Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Techno Azma, Accredited Laboratory in Fats and Oils, Tehran, Iran.
authors: Parviz
authors: P
authors: Kavousi
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000032:analysis; D057230:Limit of Detection; D000073878:Palm Oil; D010938:Plant Oils / Q000032:analysis*
chemical_list: D010938:Plant Oils; D000073878:Palm Oil; C008046:5-hydroxymethylfurfural; D005662:Furaldehyde
keywords: 5-Hydroxymethylfurfural (HMF); Accuracy; Crude palm oil (CPO); Method validation; Precision; Recovery