title: Polymerase chain reaction based detection of Toxoplasma gondii from lymph node aspirates of dogs.
abstract: The purpose of the study was to detect DNA of Toxoplasma gondii in lymph node aspirates of dogs using amplification of SAG-2 gene fragment. Blood and lymph node aspirates were collected from 20 clinically ill dogs of different age groups. Lymph node aspirates were analysed with polymerase chain reaction (PCR). Two numbers of dogs were found to be positive by PCR. The PCR positive dogs showed modified direct agglutination test (MDAT) titre of 1:512 and 1:4,096. The remaining sera samples showed the titre value of less than 1:64 by MDAT and were also negative by PCR. This study indicates that lymph node aspirates could be an alternative ideal specimen for the detection of T. gondii.
authors: Department of Veterinary Epidemiology and Preventive Medicine, Madras Veterinary College, Vepery, Chennai, 600 007 Tamilnadu India.
authors: M
authors: M
authors: Vijaya Bharathi
authors: Post Graduate Research Institute in Animal Sciences, Kattupakkam, Kattangulathur, 603 203 Tamil Nadu India.
authors: C
authors: C
authors: Sreekumar
authors: Division of Medical Entomology, Defence Research Laboratory, Defence Research & Development Organization (DRDO), Post Bag No: 2, Tezpur, Assam 784 001 India.
authors: C
authors: C
authors: Rajendran
keywords: Dogs; Lymph node aspirates; MDAT; PCR; Toxoplasma gondii