title: Cytotoxicity of newly developed pozzolan cement and other root-end filling materials on human periodontal ligament cell.
abstract: OBJECTIVE
  The purpose of this study was to evaluate in vitro cytotoxicity of the pozzolan cement and other root-end filling materials using human periodontal ligament cell.
  
  
  METHODS
  Endocem (Maruchi), white ProRoot MTA (Dentsply), white Angelus MTA (Angelus), and Super EBA (Bosworth Co.) were tested after set completely in an incubator at 37℃ for 7 days, Endocem was tested in two ways: 1) immediately after mixing (fresh specimens) and 2) after setting completely like other experimental materials. The methods for assessment included light microscopic examination, cell counting and WST-1 assay on human periodontal ligament cell.
  
  
  RESULTS
  In the results of microscopic examination and cell counting, Super EBA showed significantly lower viable cell than any other groups (p < 0.05). As the results of WST-1 assay, compared with untreated control group, there was no significant cell viability of the Endocem group. However, the fresh mixed Endocem group had significantly less cell viability. The cells exposed to ProRoot MTA and Angelus MTA showed the highest viability, whereas the cells exposed to Super EBA displayed the lowest viability (p < 0.05).
  
  
  CONCLUSIONS
  The cytotoxicity of the pozzolan cement (Endocem) was comparable with ProRoot MTA and Angelus MTA. Considering the difficult manipulation and long setting time of ProRoot MTA and Angelus MTA, Endocem can be used as the alternative of retrofilling material.
authors: Department of Conservative Dentistry Gangnam Severance Dental Hospital, Yonsei University College of Dentistry, Seoul, Korea.
authors: Minju
authors: M
authors: Song
authors: Department of Conservative Dentistry, Yonsei University College of Dentistry, Seoul, Korea.
authors: Tae-Sun
authors: TS
authors: Yoon
authors: Department of Conservative Dentistry, Yonsei University College of Dentistry, Seoul, Korea.
authors: Sue-Youn
authors: SY
authors: Kim
authors: Microscope Center, Department of Conservative Dentistry and Oral Science Research Center, Yonsei University College of Dentistry, Seoul, Korea.
authors: Euiseong
authors: E
authors: Kim
keywords: Cytotoxicity; Endocem; Pozzolan cement; Root-end filling material; WST-1 assay