title: Direct detection of circulating microRNAs in serum of cancer patients by coupling protein-facilitated specific enrichment and rolling circle amplification.
abstract: We have developed a simple method for direct detection of circulating microRNAs in serum by using the p19 protein-functionalized magnetic beads (PFMBs) for specific enrichment and rolling circle amplification (RCA). The detection limit for microRNAs is 1 fM. Therefore, the proposed method has the potential of being used in the analysis of circulating microRNAs and clinical diagnosis.
authors: The Key Lab of Analysis and Detection for Food Safety of MOE and Fujian Provincial, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350108, China. hhyang@fio.org.cn.
authors: Cheng-Yi
authors: CY
authors: Hong
authors: Xian
authors: X
authors: Chen
authors: Juan
authors: J
authors: Li
authors: Jing-Hua
authors: JH
authors: Chen
authors: Guonan
authors: G
authors: Chen
authors: Huang-Hao
authors: HH
authors: Yang
mesh_terms: D006801:Humans; D060328:Magnetic Phenomena; D035683:MicroRNAs / Q000097:blood* / Q000737:chemistry; D009369:Neoplasms / Q000097:blood*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D014764:Viral Proteins / Q000737:chemistry
chemical_list: D035683:MicroRNAs; D014764:Viral Proteins