title: SNAP25 ameliorates sensory deficit in rats with spinal cord transection.
abstract: Spinal cord injury causes sensory loss below the level of lesion. Synaptosomal-associated protein 25 (SNAP25) is a t-SNARE protein essential for exocytosis and neurotransmitter release, but its role in sensory functional recovery has not been determined. The aim of the present study is therefore to investigate whether SNAP25 can promote sensory recovery. By 2D proteomics, we found a downregulation of SNAP25 and then constructed two lentiviral vectors, Lv-exSNAP25 and Lv-shSNAP25, which allows efficient and stable RNAi-mediated silencing of endogenous SNAP25. Overexpression of SNAP25 enhanced neurite outgrowth in vitro and behavior response to thermal and mechanical stimuli in vivo, while the silencing of SNAP25 had the opposite effect. These results suggest that SNAP25 plays a crucial role in sensory functional recovery following spinal cord injury (SCI). Our study therefore provides a novel target for the management of SCI for sensory dysfunction.
authors: Department of Anesthesiology and Institute of Neurological Disease, Translation Neuroscience Center, The State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, People's Republic of China.
authors: Wei
authors: W
authors: Wang
authors: Fang
authors: F
authors: Wang
authors: Jia
authors: J
authors: Liu
authors: Wei
authors: W
authors: Zhao
authors: Qi
authors: Q
authors: Zhao
authors: Mu
authors: M
authors: He
authors: Bao-Jiang
authors: BJ
authors: Qian
authors: Yang
authors: Y
authors: Xu
authors: Ran
authors: R
authors: Liu
authors: Su-Juan
authors: SJ
authors: Liu
authors: Wei
authors: W
authors: Liu
authors: Jin
authors: J
authors: Liu
authors: Xin-Fu
authors: XF
authors: Zhou
authors: Ting-Hua
authors: TH
authors: Wang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D015536:Down-Regulation; D005089:Exocytosis / Q000502:physiology; D008297:Male; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000502:physiology*; D013119:Spinal Cord Injuries / Q000503:physiopathology*; D050825:Synaptosomal-Associated Protein 25 / Q000235:genetics* / Q000378:metabolism*
chemical_list: D050825:Synaptosomal-Associated Protein 25