title: Rapid detection of Mycoplasma pneumoniae and its macrolide-resistance mutation by Cycleave PCR.
abstract: Mycoplasma pneumoniae is a common cause of community-acquired pneumonia, which is often empirically treated with macrolides such as erythromycin and azithromycin. Recent studies have found a gradual increase in the numbers of macrolide-resistant strains due to mutations in the 23S rRNA gene. A2063G is the most common mutation, followed by A2064G. We developed a Cycleave PCR method for detecting macrolide-resistant strains of M. pneumoniae. With use of this method, the resistant strains can be quickly separated from the susceptible ones. In this work, via this method, both M. pneumoniae and resistance mutants were successfully identified from 101 clinical isolates as well as from 136 nasopharyngeal specimens. The findings of this study may allow clinicians to determine the treatment plan more rapidly and may provide a convenient method to conduct surveillance for genetic mutations conferring antibiotic resistance.
authors: Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, PR China; Key Laboratory of Clinical Pharmacology of Antibiotics, Ministry of Health, Shanghai 200040, PR China.
authors: Yang
authors: Y
authors: Liu
authors: Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, PR China; Key Laboratory of Clinical Pharmacology of Antibiotics, Ministry of Health, Shanghai 200040, PR China.
authors: Xinye
authors: X
authors: Ye
authors: Shanghai Children's Hospital, Shanghai Jiaotong University, Shanghai, 200040, PR China.
authors: Hong
authors: H
authors: Zhang
authors: Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, PR China; Key Laboratory of Clinical Pharmacology of Antibiotics, Ministry of Health, Shanghai 200040, PR China.
authors: Zhen
authors: Z
authors: Wu
authors: Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, PR China; Key Laboratory of Clinical Pharmacology of Antibiotics, Ministry of Health, Shanghai 200040, PR China. Electronic address: xuxiaogang@fudan.edu.cn.
authors: Xiaogang
authors: X
authors: Xu
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002648:Child; D002675:Child, Preschool; D024881:Drug Resistance, Bacterial; D006801:Humans; D018942:Macrolides / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods; D009177:Mycoplasma pneumoniae / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D011019:Pneumonia, Mycoplasma / Q000175:diagnosis* / Q000382:microbiology; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D018942:Macrolides; D012338:RNA, Ribosomal, 23S
keywords: CycleavePCR; Detect; Macrolide resistance; Mycoplasma pneumoniae