title: Listeria monocytogenes: cultivation and laboratory maintenance.
abstract: This unit describes general procedures for the lab cultivation and storage of the Gram-positive facultative intracellular bacterium Listeria monocytogenes. The basic protocols are relevant for a wide scope of applications including microbial genetics and both in vitro and in vivo infection studies. Commonly used L. monocytogenes strains, serotypes, and growth parameters are also discussed.
authors: Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, Kentucky.
authors: Grant S
authors: GS
authors: Jones
authors: Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, Kentucky.
authors: Sarah E F
authors: SEF
authors: D'Orazio
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D008089:Listeria monocytogenes / Q000254:growth & development* / Q000302:isolation & purification; D011309:Preservation, Biological / Q000379:methods*
keywords: Gram-positive; Listeria monocytogenes; intracellular pathogen; listeriosis