title: Proper heat shock pretreatment reduces acute liver injury induced by carbon tetrachloride and accelerates liver repair in mice.
abstract: Whether proper heat shock preconditioning can reduce liver injury and accelerate liver repair after acute liver injury is worth study. So mice received heat shock preconditioning at 40°C for 10 minutes (min), 20 min or 30 min and recovered at room temperature for 8 hours (h) under normal feeding conditions. Then acute liver injury was induced in the heat shock-pretreated mice and unheated control mice by intraperitoneal (i.p.) injection of carbon tetrachloride (CCl4). Hematoxylin and eosin (H&E) staining, serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels and the expression levels of heat shock protein 70 (HSP70), cytochrome P450 1A2 (CYP1A2) and proliferating cell nuclear antigen (PCNA) were detected in the unheated control mice and heat shock-pretreated mice after CCl4 administration. Our results showed that heat shock preconditioning at 40°C for 20 min remarkably improved the mice's survival rate (P<0.05), lowered the levels of serum AST and ALT (P<0.05), induced HSP70 (P<0.01), CYP1A2 (P<0.01) and PCNA (P<0.05) expression, effectively reduced liver injury (P<0.05) and accelerated the liver repair (P<0.05) compared with heat shock preconditioning at 40°C for 10 min or 30 min in the mice after acute liver injury induced by CCl4 when compared with the control mice. Our results may be helpful in further investigation of heat shock pretreatment as a potential clinical approach to target liver injury.
authors: Molecular Medicine Key Laboratory of Liver Injury and Repair, Medical College, Henan University of Science and Technology, An hui road 31, Jian xi district, Luoyang 471003, P.R. China.
authors: San-Qiang
authors: SQ
authors: Li
authors: Molecular Medicine Key Laboratory of Liver Injury and Repair, Medical College, Henan University of Science and Technology, An hui road 31, Jian xi district, Luoyang 471003, P.R. China.
authors: Dong-Mei
authors: DM
authors: Wang
authors: Department of Environmental Engineering and Chemistry, Luoyang Insititute of Science and Technology, Wang cheng road 90, Luo long district, Luoyang 471023, P.R. China.
authors: You-Ju
authors: YJ
authors: Shu
authors: Molecular Medicine Key Laboratory of Liver Injury and Repair, Medical College, Henan University of Science and Technology, An hui road 31, Jian xi district, Luoyang 471003, P.R. China.
authors: Xue-Dong
authors: XD
authors: Wan
authors: Molecular Medicine Key Laboratory of Liver Injury and Repair, Medical College, Henan University of Science and Technology, An hui road 31, Jian xi district, Luoyang 471003, P.R. China.
authors: Zheng-Shun
authors: ZS
authors: Xu
authors: Department of Biological Engineering, Huanghuai University, Kai yuan road 6, Yi cheng district, Zhumadian 463000, P.R. China.
authors: En-Zhong
authors: EZ
authors: Li
keywords: CCl4; CYP1A2; HSP70; PCNA; acute liver injury; heat shock pretreatment