title: Characterization of human immunodeficiency virus type 1 CRF01_AE env genes derived from recently infected Thai individuals.
abstract: Transmitted/founder virus is responsible for the establishment of human immunodeficiency virus type 1 (HIV-1) infection and induces primary anti-HIV-1 immune responses; therefore, it is important to study the viral population to understand the early events of HIV-1 infection. We amplified HIV-1 env genes from sera derived from recently infected Thai individuals, and established envelope glycoproteins (Env)-recombinant viruses. Generated Env-recombinant viruses were tested for their neutralization susceptibility to neutralizing human monoclonal antibodies (NHMAbs) and entry inhibitors, as well as being subjected to genotypic analysis. Most recombinant viruses were susceptible to neutralization by NHMAbs to Env gp41, whereas approximately one-third of the recombinant viruses were susceptible to a NHMAb against the CD4 binding site of gp120. In addition, all env genes were classified into CRF01_AE genes and showed low genetic divergence. Taken together with our previous studies on CRF01_AE env genes derived from chronically infected Thai individuals, these results suggested that the immunological and genetic characteristics of CRF01_AE Env derived from recently infected Thai individuals were different from those derived from chronically infected individuals.
authors: Nithinart
authors: N
authors: Chaitaveep
authors: Piraporn
authors: P
authors: Utachee
authors: Shota
authors: S
authors: Nakamura
authors: Thippawan
authors: T
authors: Chuenchitra
authors: Pattama
authors: P
authors: Ekpo
authors: Naokazu
authors: N
authors: Takeda
authors: Kovit
authors: K
authors: Pattanapanyasat
authors: Masanori
authors: M
authors: Kameoka
mesh_terms: D000595:Amino Acid Sequence; D057134:Antibodies, Neutralizing / Q000276:immunology; D005838:Genotype; D015483:HIV Antibodies / Q000276:immunology; D023581:HIV Fusion Inhibitors / Q000494:pharmacology; D015658:HIV Infections / Q000453:epidemiology / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D008297:Male; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013785:Thailand / Q000453:epidemiology; D055815:Young Adult; D054299:env Gene Products, Human Immunodeficiency Virus / Q000235:genetics*
chemical_list: D057134:Antibodies, Neutralizing; D015483:HIV Antibodies; D023581:HIV Fusion Inhibitors; D054299:env Gene Products, Human Immunodeficiency Virus