title: β-diketone-cobalt complexes inhibit DNA synthesis and induce S-phase arrest in rat C6 glioma cells.
abstract: β-diketone-cobalt complexes, a family of newly synthesized non-platinum metal compounds, exhibit potential antitumor activity; however, the antitumor mechanism is unclear. The current study investigated the mechanism by which β-diketone-cobalt complexes inhibit rat C6 glioma cell proliferation. It was found that β-diketone-cobalt complexes suppress rat C6 glioma cell viability in a dose-dependent manner (3.125-100 μg/ml). In rat C6 glioma cells, the IC50value of β-diketone-cobalt complexes was 24.7±3.395 μg/ml and the IC10value was 4.37±1.53 μg/ml, indicating a strong inhibitory effect. Further investigation suggested that β-diketone-cobalt complexes inhibit rat C6 glioma cell proliferation, which is associated with S-phase arrest and DNA synthesis inhibition. During this process, β-diketone-cobalt complexes decreased cyclin A expression and increased cyclin E and p21 expression. In addition, β-diketone-cobalt complexes exhibit a stronger antitumor capability than the antineoplastic agent, 5-fluorouracil.
authors: China-Japan Union Hospital, Jilin University, Changchun, Jilin 130033, P.R. China.
authors: Kaizhi
authors: K
authors: Zhang
authors: China-Japan Union Hospital, Jilin University, Changchun, Jilin 130033, P.R. China.
authors: Xingli
authors: X
authors: Zhao
authors: China-Japan Union Hospital, Jilin University, Changchun, Jilin 130033, P.R. China.
authors: Junzhi
authors: J
authors: Liu
authors: China-Japan Union Hospital, Jilin University, Changchun, Jilin 130033, P.R. China.
authors: Xiangyang
authors: X
authors: Fang
authors: China-Japan Union Hospital, Jilin University, Changchun, Jilin 130033, P.R. China.
authors: Xuepeng
authors: X
authors: Wang
authors: Key Laboratory of Polyoxometalate Science of Ministry of Education, Northeast Normal University, Changchun, Jilin 130024, P.R. China.
authors: Xiaohong
authors: X
authors: Wang
authors: China-Japan Union Hospital, Jilin University, Changchun, Jilin 130033, P.R. China.
authors: Rui
authors: R
authors: Li
keywords: DNA synthesis; cell cycle; glioma; β-diketone-cobalt complexes