title: Two functionally distinct myeloid dendritic cell subpopulations are present in bovine blood.
abstract: Immature myeloid (m)DCs circulating in the blood of cattle have been defined as lineage negative (Lin(-))MHCII(+)CD11c(+)CD205(+) cells. Lin(-)MHCII(+)CD11c(+)CD205(+) mDCs (0.2% blood mononuclear cells) isolated from bovine blood were heterogeneous in cell size and CD205 expression. Using highspeed cell sorting, Lin(-)MHCII(+)CD11c(+)CD205(+) DCs were sorted into CD205(Hi) and CD205(Lo) subpopulations which were phenotypically distinct and differed significantly (P<0.01) in TLR gene expression. CD205(Hi) and CD205(Lo) mDCs were more efficient in macropinocytosis than monocytes and expressed no or little detectable non-specific esterase activity. CD205(Lo) mDCs efficiently activated purified allogeneic T cells and the addition of TLR agonists did not significantly alter this antigen presentation capacity. T cell activation by CD205(Lo) mDCs was associated with differential up-regulation of CD40, CD80, CD86 and TGFβ1 gene expression when compared to CD205(Hi) mDCs. In conclusion, two phenotypically and functionally distinct CD11c(+)CD205(+) mDCs were isolated from blood that had an equal capacity to acquire antigen but markedly different capacities to activate T cells.
authors: Vaccine and Infectious Disease Organization-International Vaccine Center, 120 Veterinary Road, University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada.
authors: Patricia
authors: P
authors: González-Cano
authors: Vaccine and Infectious Disease Organization-International Vaccine Center, 120 Veterinary Road, University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada.
authors: Natasa
authors: N
authors: Arsic
authors: Vaccine and Infectious Disease Organization-International Vaccine Center, 120 Veterinary Road, University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada.
authors: Yurij I
authors: YI
authors: Popowych
authors: Vaccine and Infectious Disease Organization-International Vaccine Center, 120 Veterinary Road, University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada; School of Public Health, University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada. Electronic address: philip.griebel@usask.ca.
authors: Philip J
authors: PJ
authors: Griebel
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D015703:Antigens, CD / Q000378:metabolism; D001775:Blood Circulation; D039521:CD11c Antigen / Q000378:metabolism; D002417:Cattle / Q000276:immunology*; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D037181:Lectins, C-Type / Q000378:metabolism; D015778:Minor Histocompatibility Antigens; D022423:Myeloid Cells / Q000276:immunology*; D011956:Receptors, Cell Surface / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D039521:CD11c Antigen; C093569:DEC-205 receptor; D037181:Lectins, C-Type; D015778:Minor Histocompatibility Antigens; D011956:Receptors, Cell Surface
keywords: Antigen presentation; Bovine; Co-stimulatory molecules; Myeloid dendritic cells; Toll-like receptor