title: Effect of local administration of cyclosporine A on peripheral nerve regeneration in a rat sciatic nerve transection model.
abstract: OBJECTIVE
  To assess the effect of cyclosporine A (CsA) loaded in chitosan conduit on bridging the sciatic nerve defects in a rat model.
  
  
  METHODS
  A 10 mm sciatic nerve defect was bridged using a chitosan conduit filled with 10 μl carrier-drug dilution (10 μg/L CsA). In control group, the conduit was filled with the same volume of carrier dilution alone. The regene-rated fibers were studied 4, 8 and 12 weeks after surgery.
  
  
  RESULTS
  The functional study confirmed faster recovery of the regenerated axons in treatment group than control group (P<0.05). There was statistically significant difference of the gastrocnemius muscle weight ratios between treatment and control groups (P<0.05). Morphometric indices of regenerated fibers showed that the number and diameter of the myelinated fibers in CsA-treated animals were significantly higher than those in control group. In immunohistochemistry, the location of reactions to S-100 in CsA group was clearly more positive than control group.
  
  
  CONCLUSIONS
  CsA loaded in a chitosan conduit results in improvement of functional recovery and quantitative morphometric indices of sciatic nerve. It is easily available without any complications compared with its systemic administration.
authors: Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia 57153-1177, Iran.
authors: Rahim
authors: R
authors: Mohammadi
authors: Hadi
authors: H
authors: Heydarian
authors: Keyvan
authors: K
authors: Amini
mesh_terms: D000818:Animals; D048271:Chitosan; D016572:Cyclosporine / Q000008:administration & dosage* / Q000494:pharmacology; D007150:Immunohistochemistry; D009416:Nerve Regeneration / Q000187:drug effects*; D051381:Rats; D012584:Sciatic Nerve / Q000737:chemistry / Q000293:injuries*
chemical_list: D016572:Cyclosporine; D048271:Chitosan