title: Protocol and cell responses in three-dimensional conductive collagen gel scaffolds with conductive polymer nanofibres for tissue regeneration.
abstract: It has been established that nerves and skeletal muscles respond and communicate via electrical signals. In regenerative medicine, there is current emphasis on using conductive nanomaterials to enhance electrical conduction through tissue-engineered scaffolds to increase cell differentiation and tissue regeneration. We investigated the role of chemically synthesized polyaniline (PANI) and poly(3,4-ethylenedioxythiophene) (PEDOT) conductive polymer nanofibres for conductive gels. To mimic a naturally derived extracellular matrix for cell growth, type I collagen gels were reconstituted with conductive polymer nanofibres and cells. Cell viability and proliferation of PC-12 cells and human skeletal muscle cells on these three-dimensional conductive collagen gels were evaluated in vitro. PANI and PEDOT nanofibres were found to be cytocompatible with both cell types and the best results (i.e. cell growth and gel electrical conductivity) were obtained with a low concentration (0.5 wt%) of PANI. After 7 days of culture in the conductive gels, the densities of both cell types were similar and comparable to collagen positive controls. Moreover, PC-12 cells were found to differentiate in the conductive hydrogels without the addition of nerve growth factor or electrical stimulation better than collagen control. Importantly, electrical conductivity of the three-dimensional gel scaffolds increased by more than 400% compared with control. The increased conductivity and injectability of the cell-laden collagen gels to injury sites in order to create an electrically conductive extracellular matrix makes these biomaterials very conducive for the regeneration of tissues.
authors: Biological Engineering Program, Faculty of Engineering , King Mongkut's University of Technology Thonburi , Bangkok 10140 , Thailand.
authors: Sirinrath
authors: S
authors: Sirivisoot
authors: Terumo BCT , Lakewood, CO 80215 , USA.
authors: Rajesh
authors: R
authors: Pareta
authors: Wake Forest Institute for Regenerative Medicine , Wake Forest University School of Medicine , Winston-Salem, NC 27157 , USA.
authors: Benjamin S
authors: BS
authors: Harrison
keywords: PC-12; human skeletal muscle cells; nanomaterials; poly(3,4-ethylenedioxythiopene); polyaniline