title: Evaluation of the Petrifilm™ EB and TEMPO(®) EB systems with ISO 21528-2:2004 method for the count of Enterobacteriaceae in milk.
abstract: The development of alternative microbiological techniques is driven by the necessity to meet the current needs to deliver rapid results in the manufacturing process of foods, but it is important that these methods be evaluated for each application. The objective of the present study was to assess the Petrifilm™ EB and the TEMPO(®) EB systems with ISO 21528-2:2004 for the count of Enterobacteriaceae in pasteurized and UHT milk samples. We analyzed the microflora of 141 pasteurized milk samples, 15 samples of artificially contaminated pasteurized milk and 15 samples of artificially contaminated UHT milk. Investigation of the method Petrifilm™ EB and ISO 21528:2 regression analysis showed a high correlation in the samples, r = 0.90 for the microflora of pasteurized milk, r = 0.98 for artificially contaminated pasteurized milk and r = 0.99 for the artificially contaminated UHT milk. In evaluating the system TEMPO EB ® method and ISO 21528:2 correlation was also significant in the analyzed samples, with r = 0.86 for the microflora of pasteurized milk, r = 0.96 for artificially contaminated pasteurized milk and r = 0.99 for artificially contaminated UHT milk. No statistically significant differences were observed between the three methods conducted to analyze artificially contaminated pasteurized and UHT milk at three inoculum levels. In conclusion, the Petrifilm™ EB system and the TEMPO(®) EB system may be an alternative to the ISO 21528-2:2004 for the Enterobacteriaceae assay for milk as because of the ease-of-operation and the time reduction achieved for conducting the microbiological assay using these systems.
authors: Departamento de Ciência e Tecnologia de Alimentos, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Brazil.
authors: Andréia
authors: A
authors: Cirolini
authors: Departamento de Ciência e Tecnologia de Alimentos, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Brazil.
authors: Marília
authors: M
authors: Miotto
authors: Departamento de Ciência e Tecnologia de Alimentos, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Brazil.
authors: Fernanda Morgana
authors: FM
authors: Machado
authors: Departamento de Ciência e Tecnologia de Alimentos, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Brazil.
authors: Helen Silvestre
authors: HS
authors: da Silva
authors: Departamento de Informática e Estatística, Centro de Tecnologia, Universidade Federal de Santa Catarina, Brazil.
authors: Paulo José
authors: PJ
authors: Ogliari
authors: Departamento de Ciência e Tecnologia de Alimentos, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Brazil.
authors: Cleide Rosana Werneck
authors: CR
authors: Vieira
mesh_terms: D000818:Animals; D058491:Bacterial Load / Q000379:methods*; D004755:Enterobacteriaceae / Q000302:isolation & purification*; D005516:Food Microbiology / Q000379:methods*; D008892:Milk / Q000382:microbiology*; D061352:Pasteurization / Q000379:methods
keywords: Enterobacteriaceae; ISO 21528-2:2004; alternative methods; count; milk