title: Fine mapping and metabolic and physiological characterization of the glume glaucousness inhibitor locus Iw3 derived from wild wheat.
abstract: This research provided the first view of metabolic and physiological effect of a tissue-specific glaucousness inhibitor in wheat and laid foundation for map-based cloning of the Iw3 locus. Cuticular wax constitutes the outermost layer of plant skin, and its composition greatly impacts plant appearance and plant-environment interaction. Epicuticular wax in the upper part of adult wheat plants can form the glaucousness, which is associated with drought tolerance. In this research, we characterized a glume-specific glaucousness inhibitor, Iw3, by fine mapping, physiological, and molecular approaches. Iw3 inhibits glaucousness formation by altering wax composition. Compared to the wild type, Iw3 eliminated β-diketone, reduced 47 % primary alcohols, but increased aldehyde 400-fold and alkanes fivefold, which led to 30 % reduction of total glume wax load. Loss of the glaucousness increased cuticle permeability, suggesting an important role in drought sensitivity. Genetically, the glaucousness-inhibiting effect by Iw3 is partially dominant in a dosage-dependent manner. We localized the Iw3 locus within a 0.13-cM interval delimited by marker loci Xpsp3000 and XWL3096. Of the 53 wax genes assayed, we detected transcription changes in nine genes by Iw3, downregulation of Cer4-1 and upregulation of other five Cer4 and three KCS homologs. All these results provided initial insights into Iw3-mediated regulation of wax metabolism and paved way for in-depth characterization of the Iw3 locus and the glaucousness-related β-diketone pathway.
authors: College of Agronomy, Northwestern A&F University, Yangling, 712100, Shaanxi, China.
authors: Jing
authors: J
authors: Wang
authors: Wanlong
authors: W
authors: Li
authors: Wei
authors: W
authors: Wang
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D056426:Genetic Loci; D005819:Genetic Markers; D007178:Inbreeding; D010539:Permeability; D010641:Phenotype; D020161:Physical Chromosome Mapping / Q000379:methods*; D019441:Plant Epidermis / Q000648:ultrastructure; D019655:Quantitative Trait, Heritable; D014908:Triticum / Q000235:genetics* / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D014885:Waxes / Q000378:metabolism
chemical_list: D005819:Genetic Markers; D014885:Waxes