title: Expression of the transcription factor Hes3 in the mouse and human ocular surface, and in pterygium.
abstract: OBJECTIVE
  In this work we examined the presence of the neural stem cell biomarker Hairy and Enhancer of Split 3 (Hes3) in the anterior eye segment and in the aberrant growth condition of the conjunctiva pterygium. Further, we studied the response of Hes3 to irradiation.
  
  
  METHODS
  Adult mouse and human corneoscleral junction and conjunctiva, as well as human pterygium were prepared for immunohistochemical detection of Hes3 and other markers. Total body irradiation was used to study the changes in the pattern of Hes3 expression.
  
  
  RESULTS
  The adult rodent and human eye as well as pterygium, contain a population of cells expressing Hes3. In the human eye, Hes3-expressing (Hes3+) cells are found predominantly in the subconjunctival space spanning over the limbus where they physically associate with blood vessels. The cytoarchitecture of Hes3 + cells is similar to those previously observed in the adult central nervous system. Furthermore, irradiation reduces the number of Hes3 + cells in the subconjunctival space. In contrast, irradiation strongly promotes the nuclear localization of Hes3 in the ciliary body epithelium.
  
  
  CONCLUSIONS
  Our results suggest that a recently identified signal transduction pathway that regulates neural stem cells and glioblastoma cancer stem cells also operates in the ocular surface, ciliary body, and in pterygium.
authors: Department of Ophthalmology, University of Dresden , Dresden , Germany.
authors: Matina
authors: M
authors: Economopoulou
authors: Jimmy
authors: J
authors: Masjkur
authors: Frederik
authors: F
authors: Raiskup
authors: Doreen
authors: D
authors: Ebermann
authors: Sarama
authors: S
authors: Saha
authors: Mike O
authors: MO
authors: Karl
authors: Richard
authors: R
authors: Funk
authors: Jozsef
authors: J
authors: Jaszai
authors: Triantafyllos
authors: T
authors: Chavakis
authors: Monika
authors: M
authors: Ehrhart-Bornstein
authors: Lutz E
authors: LE
authors: Pillunat
authors: Leoni
authors: L
authors: Kunz-Schughart
authors: Ina
authors: I
authors: Kurth
authors: Anna
authors: A
authors: Dubrovska
authors: Andreas
authors: A
authors: Androutsellis-Theotokis
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism*; D003228:Conjunctiva / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005123:Eye / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D005786:Gene Expression Regulation / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D051379:Mice; D058990:Molecular Targeted Therapy; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000528:radiation effects; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011625:Pterygium / Q000188:drug therapy / Q000378:metabolism* / Q000503:physiopathology; D012097:Repressor Proteins; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C495457:HES3 protein, human; C411886:Hes3 protein, mouse
keywords: Hes3; conjunctiva; eye; irradiation; neural stem cells; pterygium