title: A metabolomics-based approach for predicting stages of chronic kidney disease.
abstract: Chronic kidney disease (CKD) is a major epidemiologic problem and a risk factor for cardiovascular events and cerebrovascular accidents. Because CKD shows irreversible progression, early diagnosis is desirable. Renal function can be evaluated by measuring creatinine-based estimated glomerular filtration rate (eGFR). This method, however, has low sensitivity during early phases of CKD. Cystatin C (CysC) may be a more sensitive predictor. Using a metabolomic method, we previously identified metabolites in CKD and hemodialysis patients. To develop a new index of renal hypofunction, plasma samples were collected from volunteers with and without CKD and metabolite concentrations were assayed by quantitative liquid chromatography/mass spectrometry. These results were used to construct a multivariate regression equation for an inverse of CysC-based eGFR, with eGFR and CKD stage calculated from concentrations of blood metabolites. This equation was able to predict CKD stages with 81.3% accuracy (range, 73.9-87.0% during 20 repeats). This procedure may become a novel method of identifying patients with early-stage CKD.
authors: Department of Bioengineering, Graduate School of Bioscience and Biochemistry, Tokyo Institute of Technology, 4259-G1-25 Nagatsuta-cho, Midori-ku, Yokohama 226-8502, Japan. Electronic address: kobayapi@bio.titech.ac.jp.
authors: Toshihiro
authors: T
authors: Kobayashi
authors: Global Application Development Center, Shimadzu Corporation, 1 Nishinokyo Kuwabara-cho, Nakagyo-ku, Kyoto 604-8511, Japan.
authors: Tatsunari
authors: T
authors: Yoshida
authors: Kiyokai Tanaka-Kitanoda Hospital, 707 Kitanoda, Higashi-ku, Sakai 599-8123, Japan.
authors: Tatsuya
authors: T
authors: Fujisawa
authors: Department of Bioengineering, Graduate School of Bioscience and Biochemistry, Tokyo Institute of Technology, 4259-G1-25 Nagatsuta-cho, Midori-ku, Yokohama 226-8502, Japan.
authors: Yuriko
authors: Y
authors: Matsumura
authors: Department of Bioengineering, Graduate School of Bioscience and Biochemistry, Tokyo Institute of Technology, 4259-G1-25 Nagatsuta-cho, Midori-ku, Yokohama 226-8502, Japan; Yokohama College of Pharmacy, 601 Matanocho, Totsuka-ku, Yokohama 245-0066, Japan.
authors: Toshihiko
authors: T
authors: Ozawa
authors: Department of Bioengineering, Graduate School of Bioscience and Biochemistry, Tokyo Institute of Technology, 4259-G1-25 Nagatsuta-cho, Midori-ku, Yokohama 226-8502, Japan.
authors: Hiroyuki
authors: H
authors: Yanai
authors: Department of Bioengineering, Graduate School of Bioscience and Biochemistry, Tokyo Institute of Technology, 4259-G1-25 Nagatsuta-cho, Midori-ku, Yokohama 226-8502, Japan.
authors: Atsuo
authors: A
authors: Iwasawa
authors: Department of Bioengineering, Graduate School of Bioscience and Biochemistry, Tokyo Institute of Technology, 4259-G1-25 Nagatsuta-cho, Midori-ku, Yokohama 226-8502, Japan.
authors: Toshiaki
authors: T
authors: Kamachi
authors: Kiyokai Tanaka-Kitanoda Hospital, 707 Kitanoda, Higashi-ku, Sakai 599-8123, Japan.
authors: Kouichi
authors: K
authors: Fujiwara
authors: Department of Bioengineering, Graduate School of Bioscience and Biochemistry, Tokyo Institute of Technology, 4259-G1-25 Nagatsuta-cho, Midori-ku, Yokohama 226-8502, Japan.
authors: Masahiro
authors: M
authors: Kohno
authors: Kiyokai Tanaka-Kitanoda Hospital, 707 Kitanoda, Higashi-ku, Sakai 599-8123, Japan.
authors: Noriaki
authors: N
authors: Tanaka
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002853:Chromatography, Liquid / Q000379:methods; D055316:Cystatin C / Q000097:blood* / Q000378:metabolism; D042241:Early Diagnosis; D005260:Female; D005919:Glomerular Filtration Rate; D006801:Humans; D007668:Kidney / Q000378:metabolism / Q000503:physiopathology; D016014:Linear Models; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D055432:Metabolomics / Q000379:methods*; D008875:Middle Aged; D015999:Multivariate Analysis; D051436:Renal Insufficiency, Chronic / Q000097:blood* / Q000175:diagnosis* / Q000503:physiopathology
chemical_list: D055316:Cystatin C
keywords: CKD; Cystatin C; GFR; LC/MS; OPLS