title: Macrophage metalloelastase-12 is detectable in human seminal plasma and represents a predictor for inflammatory processes in the male genital tract.
abstract: Macrophage metalloelastase-12 (MMP-12), a protein of the matrix metalloproteinase family, is involved in the breakdown of extracellular matrix in normal physiological processes as well as in disease processes. MMP-12 is almost exclusively produced by macrophages and is associated with inflammatory disorders. Giving the fact that inflammation negatively influences ejaculate parameters, we investigated a possible presence and correlation of MMP-12 in seminal plasma with parameters of the ejaculate, especially in leucocytospermic ejaculates. Forty-two patients who presented for semen analysis were assigned into four groups depending on the result of semen analysis according to the WHO guidelines 2010: normozoospermia (n = 11), OAT (n = 10), azoospermia (n = 10) and leucocytospermia (>1 mio. peroxidase-positive cells per ml) (n = 11). MMP-12 was detected by ELISA and was measurable in nearly all seminal plasma samples. Generally, MMP-12 concentrations were significantly higher in leucocytospermic samples than in nonleucocytospermic ones (P = 0.001). The MMP-12 levels between the latter nonleucocytospermic groups did not differ. Moreover, MMP-12 levels correlated with the presence of peroxidase-positive leucocytes. No correlation with CD 14 positive monocytes/macrophages was detected. In this study, we demonstrate that MMP-12 is present in seminal plasma and is correlated with inflammatory conditions in human semen and therefore may serve as predictor of ongoing inflammatory processes.
authors: Faculty of Medicine, Philipps University, Marburg, Germany.
authors: A
authors: A
authors: Urbschat
authors: P
authors: P
authors: Paulus
authors: I
authors: I
authors: Wiegratz
authors: H
authors: H
authors: Beschmann
authors: P
authors: P
authors: Hadji
authors: R
authors: R
authors: Hofmann
authors: F
authors: F
authors: Ochsendorf
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D005832:Genital Diseases, Male / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007249:Inflammation / Q000175:diagnosis / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism / Q000473:pathology; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D008297:Male; D053508:Matrix Metalloproteinase 12 / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism; D011237:Predictive Value of Tests; D012661:Semen / Q000378:metabolism*; D055101:Semen Analysis
chemical_list: D015415:Biomarkers; D018950:Lipopolysaccharide Receptors; D009195:Peroxidase; D053508:Matrix Metalloproteinase 12
keywords: Leucocytospermia; MMP-12; macrophages; seminal plasma