title: A ruthenium(II) complex capable of inducing and stabilizing bcl-2 G-quadruplex formation as a potential cancer inhibitor.
abstract: Two ruthenium(II) complexes (Ru-complexes) were synthesized and characterized in this study. The selectivity and ability of the complexes to interact with bcl-2 DNA were investigated here. It turned out that [Ru(ip)3](ClO4)2·2H2O (complex 1, ip = 1H-iminazole [4,5-f][1,10] phenanthroline) could induce and stabilize the formations of G-quadruplexes more effectively than [Ru(pip)3](ClO4)2·2H2O (complex 2, pip = 2-phenylimidazo-[4,5-f][1,10]phenanthroline) did. Considering the important role of the Ru-complex ligand in inducing and stabilizing the formations of G-quadruplex in our previous studies, we speculate that the overlarge ligand of complex 2 may block its binding affinity for G-quadruplexes. Complex 1 also induced cell apoptosis in in vitro assays. In general, this study provided potentially important information for further development of the Ru-complexes as good inducers and stabilizers of bcl-2 G-quadruplex DNA for cancer treatment.
authors: Department of Chemistry, Jinan University, Guangzhou 510632, PR China.
authors: Jingnan
authors: J
authors: Zhang
authors: Department of Chemistry, Jinan University, Guangzhou 510632, PR China.
authors: Qianqian
authors: Q
authors: Yu
authors: Department of Chemistry, Jinan University, Guangzhou 510632, PR China.
authors: Qian
authors: Q
authors: Li
authors: Department of Chemistry, Jinan University, Guangzhou 510632, PR China.
authors: Licong
authors: L
authors: Yang
authors: Department of Chemistry, Jinan University, Guangzhou 510632, PR China; School of Pharmacy, Guangdong Medical College, Zhanjiang, 524023, PR China.
authors: Lanmei
authors: L
authors: Chen
authors: Department of Chemistry, Jinan University, Guangzhou 510632, PR China.
authors: Yanhui
authors: Y
authors: Zhou
authors: Department of Chemistry, Jinan University, Guangzhou 510632, PR China. Electronic address: tliuliu@jnu.edu.cn.
authors: Jie
authors: J
authors: Liu
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000494:pharmacology*; D004273:DNA, Neoplasm / Q000037:antagonists & inhibitors* / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes; D054856:G-Quadruplexes / Q000187:drug effects; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D008024:Ligands; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D010618:Phenanthrolines / Q000737:chemistry*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D012428:Ruthenium / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D056831:Coordination Complexes; D004273:DNA, Neoplasm; D005456:Fluorescent Dyes; D008024:Ligands; D010618:Phenanthrolines; D019253:Proto-Oncogene Proteins c-bcl-2; D012428:Ruthenium
keywords: Anticancer; Bcl-2; DNA structures; G-quadruplexes; Ruthenium