title: Application of different feeding strategies in fed batch culture for pullulanase production using sago starch.
abstract: The production of pullulanase by Bacillus flavothermus KWF-1 in batch and fed batch culture were compared using 2L bioreactor. In batch culture, 0.0803 U/mL of pullulanase activity with specific activity of 0.0213 U/mg was produced by controlling the agitation speed and temperature at 200 rpm and 50 °C, respectively. Fed batch production was studied by feeding the culture with different sago starch concentrations in various feeding modes for enhanced pullulanase production. Exponential feeding mode at dilution rate of 0.01/h was the preeminent strategy for enhanced pullulanase production of 0.1710 U/mL with specific activity of 0.066 U/mg. It had shown an increment of pullulanase production and specific activity by 2.1 and 3.1-fold, respectively when compared to batch culture. Increment of pullulanase activity in exponential feeding mode improved hydrolyzation of sago starch into maltotriose and panose by 4.5 and 2.5-fold respectively compared to batch system.
authors: Industrial Biotechnology Department, Faculty of Biosciences and Bioengineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia.
authors: Shankar
authors: S
authors: R
authors: Industrial Biotechnology Department, Faculty of Biosciences and Bioengineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia. Electronic address: madihah@fbb.utm.my.
authors: Madihah
authors: M
authors: M S
authors: Industrial Biotechnology Department, Faculty of Biosciences and Bioengineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia.
authors: Shaza
authors: S
authors: E M
authors: Industrial Biotechnology Department, Faculty of Biosciences and Bioengineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia.
authors: Nur Aswati
authors: NA
authors: K O
authors: Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors: Suraini
authors: S
authors: A A
authors: Industrial Biotechnology Research Centre, SIRIM Berhad, Malaysia.
authors: Kamarulzaman
authors: K
authors: K
mesh_terms: D006026:Glycoside Hydrolases / Q000096:biosynthesis*; D013213:Starch / Q000378:metabolism*
chemical_list: D013213:Starch; D006026:Glycoside Hydrolases; C100181:pullulanase
keywords: Fed batch; Maltose; Maltotriose; Panose; Pullulanase; Sago