title: Isolation, structure determination, and antiaging effects of 2,3-pentanediol from endophytic fungus of Curcuma amada and docking studies.
abstract: An endophytic fungus was isolated from the rhizomes of Curcuma amada (Zingiberaceae), which was identified as Fusarium oxysporum on the basis of its morphological and molecular characters. Chromatographic separation and spectroscopic analysis of the fungal metabolite (chloroform extract) led to the identification of one pure compound having molecular formula C5H12O2, i.e., 2,3-pentanediol (1). Activity analysis of compound 1 demonstrated improved antiaging (antioxidant, thermotolerance) properties against Caenorhabditis elegans, in comparison to a similar, commercially available molecule i.e., 1,5-pentanediol (2). The effective (lower) concentration of 1 significantly showed (28.6%) higher survival percentage of the worms under thermal stress (37 ºC) compared to its higher concentration (25.3%), while similar trends were followed in oxidative stress where (22.2%) higher survival percentage was recorded in comparison to untreated control. The compound 1, however, lacked potential antimicrobial activity, indicating the plausible ramification of the position of OH group in such bioactive molecules. In silico evaluation of these molecules against common as well as unique targets corroborated better antiaging potential of 1 in comparison to that of 2. The results for the first time indicated that the utilization of the endophytic fungi of C. amada could, thus, be a possible source for obtaining non-plant-based bioactive compounds having broader therapeutic applications pertaining to age-related progressions.
authors: Microbial Technology and Nematology Division, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Lucknow, Uttar Pradesh, 226015, India.
authors: Sudeep
authors: S
authors: Tiwari
authors: Sailendra
authors: S
authors: Singh
authors: Pallavi
authors: P
authors: Pandey
authors: Shilpi K
authors: SK
authors: Saikia
authors: Arvind Singh
authors: AS
authors: Negi
authors: Shailendra K
authors: SK
authors: Gupta
authors: Rakesh
authors: R
authors: Pandey
authors: Suchitra
authors: S
authors: Banerjee
mesh_terms: D000375:Aging / Q000187:drug effects*; D000818:Animals; D000900:Anti-Bacterial Agents; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000187:drug effects; D030024:Curcuma / Q000382:microbiology*; D005670:Fusarium / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006018:Glycols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D062105:Molecular Docking Simulation; D008969:Molecular Sequence Data; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D027343:Rhizome / Q000382:microbiology; D017423:Sequence Analysis, RNA
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D006018:Glycols; D012337:RNA, Ribosomal, 18S; C053671:2,3-pentanediol