title: [The profile of IGF2R gene expression and H3 histone modifications in replicative cell senescence].
abstract: OBJECTIVE
  To study the profile of IGF2R expression and histone modifications in replicative cell senescence.
  
  
  METHODS
  The changes of biological characteristics of young human pulmonary fibroblast (HPF) cells [at population doubling level (PDL) 23] and aging HPF cells (at PDL50) were observed and real-time quantitative PCR was utilized to investigate human IGF2R gene expressions profile during the process of cellular aging (at different PDL). Then chromatinimmunoprecipitation-real time quantitative PCR (CHIP-QPCR) methods were conducted to analyze histone modifications of the regions around the transcriptional start site of IGF2R (H3-Ac, H3K9-tri-Me, H3K9-Ac and H3K4-tri-Me).
  
  
  RESULTS
  In contrast to young cells, the aging cells were bigger and less proliferative, their cell cycles arrest, and aging specific beta-galactosidase staining was positive. IGF2R gene expression was in positive correlation with PDL. H3-Ac, H3K9-Ac and H3K4-tri-Me were dominant in the upstream region (-0.6 kb) to the downstream region (+1.2 kb) of transcriptional start site (TSS). While in the downstream of TSS from +1.6 kb to +4.0 kb, H3K9-Ac was declined and H3K9-tri-Me was elevated in turn, but H3K4-tri-Me still prevailed in these areas.
  
  
  CONCLUSIONS
  IGF2R is related to cell replicative senescence and its gene expression is regulated by histone modification of H3. Therefore, epigenetics may play a role in cell senescence.
authors: Sichuan Center for Disease Control and Prevention, Chengdu 610041, China.
authors: Wei
authors: W
authors: Xu
authors: Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
authors: Zhi-Xiong
authors: ZX
authors: Zhuang
authors: Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
authors: Jian-Ping
authors: JP
authors: Yang
authors: Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
authors: Lin-Qing
authors: LQ
authors: Yang
authors: Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
authors: Yu-Ling
authors: YL
authors: Xu
authors: Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
authors: Wen-Juan
authors: WJ
authors: Zhang
authors: Sichuan Center for Disease Control and Prevention, Chengdu 610041, China.
authors: Yi
authors: Y
authors: Zeng
mesh_terms: D016922:Cellular Senescence; D019175:DNA Methylation; D005347:Fibroblasts / Q000166:cytology*; D015870:Gene Expression; D006657:Histones / Q000378:metabolism*; D006801:Humans; D008168:Lung / Q000166:cytology; D017527:Receptor, IGF Type 2 / Q000235:genetics / Q000378:metabolism*
chemical_list: D006657:Histones; D017527:Receptor, IGF Type 2