title: Identification of splice variants, expression analysis and single nucleotide polymorphisms of the PRMT2 gene in dairy cattle.
abstract: Protein arginine N-methyltransferase 2 (PRMT2), also named HRMT1L1, belongs to the Bovine Protein arginine N-methyltransferase (PRMT) genes which are involved in the immune response. To explore the variability of the PRMT2 gene and resistance to mastitis in cows, splice variant (SV), and single nucleotide polymorphisms (SNPs) were identified in this study. A SV (PRMT2-SV) lacking exon 7 (98-bp) of the PRMT2 gene was found in healthy and mastitis-infected mammary gland tissues. Two of four SNPs were significantly associated with bovine milk yield and protein content. Further, we estimated the relative expression of PRMT2-SV in the mammary gland tissue of dairy cattle by using quantitative real-time polymerase chain reaction. The result showed that expression of the PRMT2-SV mRNA was significantly upregulated 4.02-fold (p<0.05) in infected mammary tissues (n=5) compared to healthy tissues (n=5). Our findings reveal that PRMT2-SV may play an important role in mastitis resistance in dairy cattle. The SNPs may be used as a possible candidate SNPs for marker-assisted selection and management in Chinese Holstein cattle.
authors: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100, China.
authors: Zhixiong
authors: Z
authors: Li
authors: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100, China.
authors: Mengxing
authors: M
authors: Zhai
authors: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100, China.
authors: Hongliang
authors: H
authors: Wang
authors: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100, China.
authors: Ling
authors: L
authors: Chen
authors: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100, China.
authors: Lijun
authors: L
authors: Wang
authors: Animal Husbandry Company, Xi'an Modern Agriculture Comprehensive Development Corporation, Shaanxi 710018, China.
authors: Caixia
authors: C
authors: Ru
authors: Animal Husbandry Company, Xi'an Modern Agriculture Comprehensive Development Corporation, Shaanxi 710018, China.
authors: Ailong
authors: A
authors: Song
authors: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100, China. Electronic address: xiaolinliu2000@sina.com.
authors: Xiaolin
authors: X
authors: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D020022:Genetic Predisposition to Disease; D008321:Mammary Glands, Animal / Q000276:immunology; D008414:Mastitis, Bovine / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D008892:Milk / Q000378:metabolism; D008894:Milk Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D020033:Protein Isoforms / Q000235:genetics*; D017433:Protein Structure, Secondary; D011484:Protein-Arginine N-Methyltransferases / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D015854:Up-Regulation
chemical_list: D008894:Milk Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D011484:Protein-Arginine N-Methyltransferases; C464934:protein arginine methyltransferase 2
keywords: Dairy cow; Mastitis; PRMT2; Quantitative real-time PCR; Single nucleotide polymorphisms; Splice variant