title: [Effect of extracorporeal shock wave therapy on cartilage and subchondral bone remodeling in rabbits with ACLT-induced osteoarthritis].
abstract: OBJECTIVE
  To investigate the role of extracorporeal shock wave therapy on the protection of cartilage and subchondral bone remodeling in rabbits with osteoarthritis induced by anterior cruciate ligament transaction (ACLT).
  
  
  METHODS
  Anterior cruciate ligament transaction was performed on 24 adult rabbits to establish knee osteoarthritis models. The rabbits were randomly divided into two groups, with one group receiving extracorporeal shock wave therapy (ESWT) and the other group serving as controls. The ESWT was set at energy 0.16 MPa/time, frequency 5 Hz, 1 200 shock per side, 3 times per week, and a total of 6 times in 4 weeks. Histological observations were undertaken with articular cartilages under mascroscope and microscope; bone mineral density (BMD) was measured. The subchondral bones of femoralcondyle and tibial plateau were given a bone histomorphometry analysis. The levels of interleukin-1 beta (IL-1beta), tumor necrosis factor alpha (TNF-alpha) and nitric oxide (NO) were detected by ELISA. The expressions of matrix metalloproteinases (MMP-1, MMP 3, MMP-13) and tissue inhibitor of metalloproteinases 1 (TIMP-1) in cartilage were determined by RT-PCR.
  
  
  RESULTS
  Lower levels of histological result in medial femoral condyles (by both mascroscope and microscope examinations) and higher levels of BMD (in distal femora) were found in the ESWT treated rabbits compared with the controls (P < 0.05). The ESWT treated rabbits also had decreased trabecular bone relative volume and trabecular bone thickness, increased bone separation, lower levels of expressions of MMP-1 and MMP-3, and higher levels of TIMP-1.
  
  
  CONCLUSIONS
  ESWT can protect cartilage from damages and prevent subchondral sclerosis through regulating MMP-1, MMP-3 and TIMP-1 in cartilages and modulating subchondral bone metabolisms.
authors: Center of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Pu
authors: P
authors: Wang
authors: Center of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Chuan
authors: C
authors: Liu
authors: Center of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Xiao-Tian
authors: XT
authors: Yang
authors: Center of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Xiao-Fei
authors: XF
authors: Wei
authors: Center of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Yu-Jing
authors: YJ
authors: Zhou
authors: Center of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Lin
authors: L
authors: Yang
authors: Center of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Cheng-Qi
authors: CQ
authors: He
mesh_terms: D000818:Animals; D016118:Anterior Cruciate Ligament / Q000473:pathology; D015519:Bone Density; D016723:Bone Remodeling; D001842:Bone and Bones; D002358:Cartilage, Articular / Q000473:pathology; D004195:Disease Models, Animal; D053583:Interleukin-1beta / Q000378:metabolism; D007719:Knee Joint / Q000473:pathology; D020782:Matrix Metalloproteinases / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D020370:Osteoarthritis, Knee / Q000628:therapy*; D011817:Rabbits; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; D019715:Tissue Inhibitor of Metalloproteinase-1; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D020782:Matrix Metalloproteinases