title: RBM10 regulates alternative splicing.
abstract: RBM10, originally called S1-1, is a nuclear RNA-binding protein with domains characteristic of RNA processing proteins. It has been reported that RBM10 constitutes spliceosome complexes and that RBM5, a close homologue of RBM10, regulates alternative splicing of apoptosis-related genes, Fas and cFLIP. In this study, we examined whether RBM10 has a regulatory function in splicing similar to RBM5, and determined that it indeed regulates alternative splicing of Fas and Bcl-x genes. RBM10 promotes exon skipping of Fas pre-mRNA as well as selection of an internal 5'-splice site in Bcl-x pre-mRNA. We propose a consensus RBM10-binding sequence at 5'-splice sites of target exons and a mechanistic model of RBM10 action in the alternative splicing.
authors: Department of Immunology, Osaka City University Graduate School of Medicine, Japan; Department of Otolaryngology, Osaka City University Graduate School of Medicine, Japan. Electronic address: ainoue@med.osaka-cu.ac.jp.
authors: Akira
authors: A
authors: Inoue
authors: Department of Psychiatry and Behavioral Sciences, Tokyo Medical and Dental University Graduate School, Japan.
authors: Naoki
authors: N
authors: Yamamoto
authors: Department of Radioisotope Center, Osaka City University Graduate School of Medicine, Japan.
authors: Masatsugu
authors: M
authors: Kimura
authors: Department of Anatomy and Cell Biology, Graduate School of Medicine, Nagoya University, Japan.
authors: Koji
authors: K
authors: Nishio
authors: Department of Otolaryngology, Osaka City University Graduate School of Medicine, Japan.
authors: Hideo
authors: H
authors: Yamane
authors: Department of Immunology, Osaka City University Graduate School of Medicine, Japan.
authors: Koichi
authors: K
authors: Nakajima
mesh_terms: D017398:Alternative Splicing; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D020169:Caspases / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000502:physiology; D016384:Consensus Sequence; D004268:DNA-Binding Proteins / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D022821:RNA Splice Sites; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000502:physiology*; D025521:Tumor Suppressor Proteins / Q000502:physiology; D019014:fas Receptor / Q000235:genetics / Q000378:metabolism
chemical_list: D051017:Apoptosis Regulatory Proteins; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; C491901:FAS protein, human; D007527:Isoenzymes; C512260:RBM10 protein, human; C473448:RBM5 protein, human; D022821:RNA Splice Sites; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D025521:Tumor Suppressor Proteins; D019014:fas Receptor; D020169:Caspases
keywords: Alternative splicing; Bcl-x; Fas; RBM10; RBM5; S1-1