title: Plasma-functionalized electrospun matrix for biograft development and cardiac function stabilization.
abstract: Cardiac tissue engineering approaches can deliver large numbers of cells to the damaged myocardium and have thus increasingly been considered as a possible curative treatment to counteract the high prevalence of progressive heart failure after myocardial infarction (MI). Optimal scaffold architecture and mechanical and chemical properties, as well as immune- and bio-compatibility, need to be addressed. We demonstrated that radio-frequency plasma surface functionalized electrospun poly(ɛ-caprolactone) (PCL) fibres provide a suitable matrix for bone-marrow-derived mesenchymal stem cell (MSC) cardiac implantation. Using a rat model of chronic MI, we showed that MSC-seeded plasma-coated PCL grafts stabilized cardiac function and attenuated dilatation. Significant relative decreases of 13% of the ejection fraction (EF) and 15% of the fractional shortening (FS) were observed in sham treated animals; respective decreases of 20% and 25% were measured 4 weeks after acellular patch implantation, whereas a steadied function was observed 4 weeks after MSC-patch implantation (relative decreases of 6% for both EF and FS).
authors: Empa, Swiss Federal Laboratories for Materials Science and Technology, 9000 St. Gallen, Switzerland; Department of Cardiovascular Surgery, University Hospital and University of Bern, 3010 Bern, Switzerland; Graduate School for Cellular and Biomedical Sciences, University of Bern, 3010 Bern, Switzerland.
authors: A G
authors: AG
authors: Guex
authors: Cardiology, Department of Medicine, University of Fribourg, 1700 Fribourg, Switzerland.
authors: A
authors: A
authors: Frobert
authors: Cardiology, Department of Medicine, University of Fribourg, 1700 Fribourg, Switzerland.
authors: J
authors: J
authors: Valentin
authors: Empa, Swiss Federal Laboratories for Materials Science and Technology, 9000 St. Gallen, Switzerland.
authors: G
authors: G
authors: Fortunato
authors: Empa, Swiss Federal Laboratories for Materials Science and Technology, 9000 St. Gallen, Switzerland.
authors: D
authors: D
authors: Hegemann
authors: Cardiology, Department of Medicine, University of Fribourg, 1700 Fribourg, Switzerland.
authors: S
authors: S
authors: Cook
authors: Department of Cardiovascular Surgery, University Hospital and University of Bern, 3010 Bern, Switzerland.
authors: T P
authors: TP
authors: Carrel
authors: Department of Cardiovascular Surgery, University Hospital and University of Bern, 3010 Bern, Switzerland.
authors: H T
authors: HT
authors: Tevaearai
authors: Cardiology, Department of Medicine, University of Fribourg, 1700 Fribourg, Switzerland. Electronic address: marie-noelle.giraud@unifr.ch.
authors: M N
authors: MN
authors: Giraud
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006334:Heart Function Tests; D008297:Male; D009203:Myocardial Infarction / Q000150:complications / Q000503:physiopathology; D051381:Rats; D011917:Rats, Inbred Lew; D023822:Tissue Engineering; D054457:Tissue Scaffolds
keywords: Cardiac tissue engineering; Echocardiography: cell therapy; Electrospinning; In vivo functional evaluation; Plasma polymerization