title: Membrane palmitoylated protein 3 promotes hepatocellular carcinoma cell migration and invasion via up-regulating matrix metalloproteinase 1.
abstract: Membrane associated guanylate kinase (MAGUK) family, has been extensively studied in cellular adhesion and signal transduction at sites of cell–cell contact. Recently, growing attention has been paid to its role in the initiation and progression of various cancers. However, its role in hepatocellular carcinoma (HCC) has been rarely investigated. In this study, we found that membrane palmitoylated protein 3 (MPP3), a member of MAGUK family, was significantly up-regulated in both high metastatic potential cell lines and clinical tissue samples of HCC, and the most significant increase was observed in the tumors invading the portal veins. Higher level of MPP3 correlated with poorer survival of patients with HCC. Forced expression of MPP3 significantly enhanced HCC cell migration and invasion, whereas knockdown of this gene inhibited this oncogenic effect. Mechanismly, we found that MPP3 promoted HCC cell migration and invasion via up-regulating matrix metalloproteinase 1 (MMP1). These findings indicate that MPP3 play an important role in HCC metastasis by promoting cell migration and invasion, suggesting that it may serve as a novel prognostic marker and molecular target for therapy of HCC.
authors: The State Key Laboratory of Genetic Engineering, Fudan University, Shanghai, PR China; Joint Laboratory of Immunogenomics, Zhoushan Hospital-BIG/CAS, Zhoushan, Zhejiang, PR China.
authors: Haijie
authors: H
authors: Ma
authors: The State Key Laboratory of Genetic Engineering, Fudan University, Shanghai, PR China.
authors: Hao
authors: H
authors: Cai
authors: Joint Laboratory of Immunogenomics, Zhoushan Hospital-BIG/CAS, Zhoushan, Zhejiang, PR China.
authors: Yongkui
authors: Y
authors: Zhang
authors: The State Key Laboratory of Genetic Engineering, Fudan University, Shanghai, PR China.
authors: Jiaxue
authors: J
authors: Wu
authors: Joint Laboratory of Immunogenomics, Zhoushan Hospital-BIG/CAS, Zhoushan, Zhejiang, PR China.
authors: Xiaoguang
authors: X
authors: Liu
authors: The State Key Laboratory of Genetic Engineering, Fudan University, Shanghai, PR China.
authors: Jie
authors: J
authors: Zuo
authors: The State Key Laboratory of Genetic Engineering, Fudan University, Shanghai, PR China.
authors: Wei
authors: W
authors: Jiang
authors: The State Key Laboratory of Genetic Engineering, Fudan University, Shanghai, PR China.
authors: Guoqing
authors: G
authors: Ji
authors: The State Key Laboratory of Genetic Engineering, Fudan University, Shanghai, PR China.
authors: Yuanyuan
authors: Y
authors: Zhang
authors: The State Key Laboratory of Genetic Engineering, Fudan University, Shanghai, PR China.
authors: Chao
authors: C
authors: Liu
authors: Joint Laboratory of Immunogenomics, Zhoushan Hospital-BIG/CAS, Zhoushan, Zhejiang, PR China.
authors: Wangyu
authors: W
authors: Zhu
authors: The State Key Laboratory of Genetic Engineering, Fudan University, Shanghai, PR China. Electronic address: longyu@fudan.edu.cn.
authors: Long
authors: L
authors: Yu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000201:enzymology* / Q000473:pathology; D002465:Cell Movement / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D055785:Gene Knockdown Techniques; D051528:Guanylate Kinases / Q000378:metabolism*; D064593:Heterografts; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000201:enzymology* / Q000473:pathology; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D009361:Neoplasm Invasiveness; D060888:Real-Time Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D051528:Guanylate Kinases; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1