title: A recombinant matrix metalloproteinase protein from Gnathostoma spinigerum for serodiagnosis of neurognathostomiasis.
abstract: Neurognathostomiasis is a severe form of human gnathostomiasis which can lead to disease and death. Diagnosis of neurognathostomiasis is made presumptively by using clinical manifestations. Immunoblotting, which recognizes antigenic components of molecular mass 21 kDa and 24 kDa in larval extracts of Gnathostoma spinigerum (Gs 21/24), has high sensitivity and specificity for diagnosis of neurognathostomiasis. However, only very small amounts of the Gs 21/24 antigens can be prepared from parasites harvested from natural or experimental animals. To overcome this problem, we recently produced a recombinant matrix metalloproteinase (rMMP) protein from G. spinigerum. In this study, we evaluated this rMMP alongside the Gs 21/24 antigens for serodiagnosis of human neurognathostomiasis. We studied sera from 40 patients from Srinagarind Hospital, Khon Kaen University, Thailand, with clinical criteria consistent with those of neurognathostomiasis, and sera from 30 healthy control adults from Thailand. All sera were tested for specific IgG antibodies against both G. spinigerum crude larval extract and rMMP protein using immunoblot analysis. The sensitivity and specificity for both antigenic preparations were all 100%. These results show that G. spinigerum rMMP protein can be used as an alternative diagnostic antigen, in place of larval extract, for serodiagnosis of neurognathostomiasis.
authors: Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen 40002, Thailand. ; Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Penchom
authors: P
authors: Janwan
authors: Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen 40002, Thailand. ; Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Pewpan M
authors: PM
authors: Intapan
authors: Department of Parasitology, National Institute of Infectious Diseases, Tokyo, Japan.
authors: Hiroshi
authors: H
authors: Yamasaki
authors: Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen 40002, Thailand. ; Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Porntip
authors: P
authors: Laummaunwai
authors: Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen 40002, Thailand. ; Department of Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Kittisak
authors: K
authors: Sawanyawisuth
authors: Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Chaisiri
authors: C
authors: Wongkham
authors: Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
authors: Chatchai
authors: C
authors: Tayapiwatana
authors: Department of Surgery, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Amnat
authors: A
authors: Kitkhuandee
authors: Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen 40002, Thailand. ; Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Viraphong
authors: V
authors: Lulitanond
authors: Research Division, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Yukifumi
authors: Y
authors: Nawa
authors: Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen 40002, Thailand. ; Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Wanchai
authors: W
authors: Maleewong
mesh_terms: D000328:Adult; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000235:genetics / Q000302:isolation & purification; D020807:Central Nervous System Parasitic Infections / Q000175:diagnosis* / Q000469:parasitology; D006039:Gnathostoma / Q000201:enzymology / Q000276:immunology / Q000302:isolation & purification*; D058429:Gnathostomiasis / Q000175:diagnosis* / Q000469:parasitology; D064368:Healthy Volunteers; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007074:Immunoglobulin G / Q000097:blood; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000302:isolation & purification; D010274:Parasitology / Q000379:methods*; D011446:Prospective Studies; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D013785:Thailand
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D020782:Matrix Metalloproteinases
keywords: Gnathostoma spinigerum; immunoblotting; neurognathostomiasis; recombinant matrix metalloproteinase