title: Immune activities comparison of polysaccharide and polysaccharide-protein complex from Lycium barbarum L.
abstract: Lycium barbarum L., known as wolfberry, is an important Chinese herbal medicine. In the research, we purified water-soluble polysaccharide-protein complex (LBPF4) and polysaccharide (LBPF4-OL) from the fruiting bodies of L. barbarum L. The monosaccharide and amino acid composition of LBPF4 and LBPF4-OL was elucidated with fractional acid hydrolization, GC/MC and NMR techniques. LBPF4-OL molecular weight was 181 kDa, as determined by high-performance gel-permeation chromatography (HPGPC). In vitro assay, we found that LBPF4 induced splenocyte proliferations depended on both B cells and T cells, but LBPF4-OL induced splenocyte proliferations mainly depended on B cells. ELISA results showed that both LBPF4 and LBPF4-OL significantly induced TNF-α, IL-1β and NO production on macrophage. We also found that both LBPF4 and LBPF4-OL can enhance macrophage phagocytosis. Furthermore, electrophoretic mobility shift assay (EMSA) studies suggest that LBPF4 100 μg/ml treatment can more effectively increase NF-κB activity than LBPF4-OL. Taken together, our results demonstrate that LBPF4 can enhance T, B cells and macrophages functions, but LBPF4-OL can only enhance B cells and macrophage functions. This is partly due to LBPF4 being able to more significantly enhance lymphocytes NF-κB activity.
authors: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, PR China.
authors: Xiaorui
authors: X
authors: Zhang
authors: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, PR China.
authors: Yingjie
authors: Y
authors: Li
authors: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, PR China.
authors: Junping
authors: J
authors: Cheng
authors: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, PR China.
authors: Gang
authors: G
authors: Liu
authors: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, PR China.
authors: Chunhui
authors: C
authors: Qi
authors: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, PR China. Electronic address: zhouwx@bmi.ac.cn.
authors: Wenxia
authors: W
authors: Zhou
authors: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, PR China. Electronic address: zhangyx@nic.bmi.ac.cn.
authors: Yongxiang
authors: Y
authors: Zhang
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D007155:Immunologic Factors / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000096:biosynthesis; D032305:Lycium / Q000737:chemistry*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008970:Molecular Weight; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis; D051757:PAX5 Transcription Factor / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D010940:Plant Proteins / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D007155:Immunologic Factors; D053583:Interleukin-1beta; D016328:NF-kappa B; D051757:PAX5 Transcription Factor; C497282:Pax5 protein, mouse; D010940:Plant Proteins; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide
keywords: Lycium barbarum L.; Lymphocytes; Macrophage; Nuclear factor-κB; Polysaccharide-protein complex