title: Compared with that of MUFA, a high dietary intake of n-3 PUFA does not reduce the degree of pathology in mdx mice.
abstract: Duchenne muscular dystrophy (DMD) is a severe muscle disease that affects afflicted males from a young age, and the mdx mouse is an animal model of this disease. Although new drugs are in development, it is also essential to assess potential dietary therapies that could assist in the management of DMD. In the present study, we compared two diets, high-MUFA diet v. high-PUFA diet, in mdx mice. To generate the high-PUFA diet, a portion of dietary MUFA (oleic acid) was replaced with the dietary essential n-3 PUFA α-linolenic acid (ALA). We sought to determine whether ALA, compared with oleic acid, was beneficial in mdx mice. Consumption of the high-PUFA diet resulted in significantly higher n-3 PUFA content and reduced arachidonic acid content in skeletal muscle phospholipids (PL), while the high-MUFA diet led to higher oleate content in PL. Mdx mice on the high-MUFA diet exhibited 2-fold lower serum creatine kinase activity than those on the high-PUFA diet (P< 0·05) as well as a lower body fat percentage (P< 0·05), but no significant difference in skeletal muscle histopathology results. There was no significant difference between the dietary groups with regard to phosphorylated p65 (an inflammatory marker) in skeletal muscle. In conclusion, alteration of PL fatty acid (FA) composition by the high-PUFA diet made mdx muscle more susceptible to sarcolemmal leakiness, while the high-MUFA diet exhibited a more favourable impact. These results may be important for designing dietary treatments for DMD patients, and future work on dietary FA profiles, such as comparing other FA classes and dose effects, is needed.
authors: Department of Exercise Science and Rutgers Center for Lipid Research, Rutgers University, 70 Lipman Drive, Loree Building, New Brunswick, NJ 08901, USA.
authors: Gregory C
authors: GC
authors: Henderson
authors: Department of Population Health Sciences, Virginia Polytechnic Institute and State University, 1410 Prices Fork Rd, Blacksburg, VA 24061, USA.
authors: Nicholas P
authors: NP
authors: Evans
authors: Department of Human Nutrition, Foods and Exercise, Virginia Polytechnic Institute and State University, 338 Wallace Hall, Blacksburg, VA 24061, USA.
authors: Robert W
authors: RW
authors: Grange
authors: Department of Exercise Science and Rutgers Center for Lipid Research, Rutgers University, 70 Lipman Drive, Loree Building, New Brunswick, NJ 08901, USA.
authors: Marc A
authors: MA
authors: Tuazon
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002853:Chromatography, Liquid; D003402:Creatine Kinase / Q000097:blood*; D004041:Dietary Fats / Q000378:metabolism*; D004195:Disease Models, Animal; D005229:Fatty Acids, Monounsaturated / Q000378:metabolism*; D015525:Fatty Acids, Omega-3 / Q000378:metabolism*; D007249:Inflammation / Q000378:metabolism; D008297:Male; D051379:Mice; D018101:Mice, Inbred mdx; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology; D020388:Muscular Dystrophy, Duchenne / Q000473:pathology*; D016328:NF-kappa B / Q000032:analysis; D019301:Oleic Acid / Q000378:metabolism; D010743:Phospholipids / Q000302:isolation & purification*; D010766:Phosphorylation; D010938:Plant Oils / Q000378:metabolism
chemical_list: D015415:Biomarkers; D004041:Dietary Fats; D005229:Fatty Acids, Monounsaturated; D015525:Fatty Acids, Omega-3; D016328:NF-kappa B; D010743:Phospholipids; D010938:Plant Oils; D016718:Arachidonic Acid; D019301:Oleic Acid; D003402:Creatine Kinase