title: Effects of atrazine and chlorpyrifos on cytochrome P450 in common carp liver.
abstract: Atrazine (ATR) and chlorpyrifos (CPF), widely used in agriculture, have resulted in a series of toxicological and environmental problems. We investigated the activities of the biotransformation enzymes ethoxyresorufin-O-deethylase (EROD) and pentoxyresorufin-O-deethylase (PROD), total cytochrome P450 (CYP), CYP1A mRNA level and level of tissue ATR, CPF, and their metabolites in the liver of common carp (Cyprinus carpio L.) after a 40-d exposure to CPF and ATR, alone or in combination, and a 20-d recovery. In the present study, juvenile common carp was exposed to ATR (at concentrations of 4.28, 42.8 and 428 μg L(-1)), CPF (1.16, 11.6 and 116 μg L(-1)), and ATR/CPF mixture (at concentrations of 1.13, 11.3 and 113 μg L(-1)). A general increasing trend for the activity of the biotransformation enzymes (EROD and PROD), CYP and CYP1A mRNA level was observed in the liver of common carp exposed to ATR, CPF and the ATR/CPF mixture. In addition, ATR, CPF, and their metabolites demonstrated a high accumulation in the liver. These results demonstrated that the CYP system in fish could be used as a biomarkers in evaluating the impact of ATR and CPF exposure on the common carp.
authors: College of Animal Science and Technology, Northeast Agricultural University, 59 Mucai Street, Harbin 150030, PR China; Animal Health Supervision Institute of Heilongjiang Province, 243 Haping Road, Xiangfang District, Harbin 150069, PR China.
authors: Houjuan
authors: H
authors: Xing
authors: College of Animal Science and Technology, Northeast Agricultural University, 59 Mucai Street, Harbin 150030, PR China.
authors: Ziwei
authors: Z
authors: Zhang
authors: College of Animal Science and Technology, Northeast Agricultural University, 59 Mucai Street, Harbin 150030, PR China.
authors: Haidong
authors: H
authors: Yao
authors: College of Animal Science and Technology, Northeast Agricultural University, 59 Mucai Street, Harbin 150030, PR China.
authors: Tao
authors: T
authors: Liu
authors: College of Animal Science and Technology, Northeast Agricultural University, 59 Mucai Street, Harbin 150030, PR China.
authors: Liangliang
authors: L
authors: Wang
authors: College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Harbin 150030, PR China. Electronic address: shiwenxu@neau.edu.cn.
authors: Shiwen
authors: S
authors: Xu
authors: College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Harbin 150030, PR China. Electronic address: lishu@neau.edu.cn.
authors: Shu
authors: S
authors: Li
mesh_terms: D000818:Animals; D001280:Atrazine / Q000378:metabolism*; D002347:Carps / Q000378:metabolism*; D004390:Chlorpyrifos / Q000378:metabolism*; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D029941:Fish Proteins / Q000378:metabolism; D005399:Fishes / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D010575:Pesticides / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D029941:Fish Proteins; D010575:Pesticides; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D003577:Cytochrome P-450 Enzyme System; D019363:Cytochrome P-450 CYP1A1; D004390:Chlorpyrifos; D001280:Atrazine
keywords: Atrazine; CYP system; Chlorpyrifos; Common carp; Pesticide residue