title: Induction of specific humoral and cellular immune responses in a mouse model following gene fusion of HSP70C and Hantaan virus Gn and S0.7 in an adenoviral vector.
abstract: Heat shock proteins (HSPs) display adjuvant functions when given as fusion proteins to enhance vaccination efficiency. To evaluate enhanced potency of Hantaan virus (HTNV) glycoprotein (GP) and nucleocapsid protein (NP) immunogenicity by heat shock protein 70 (HSP70), a recombinant adenovirus rAd-GnS0.7-pCAG-HSP70C expression vector was developed by genetically linking the HSP70 C-terminal gene (HSP70 359-610 aa, HSP70C) to the Gn and 0.7 kb fragment of the NP (aa1-274-S0.7). C57BL/6 mice were immunized with these recombinant adenoviral vectors. A series of immunological assays determined the immunogenicity of the recombinant adenoviral vectors. The results showed that rAd-GnS0.7-pCAG-HSP70C induced a stronger humoral and cellular immune response than other recombinant adenoviruses (rAd-GnS0.7-pCAG and rAd-GnS0.7) and the HFRS vaccine control. Animal protection experiments showed that rAd-GnS0.7-pCAG-HSP70C was effective at protecting C57BL/6 mice from HTNV infection. The results of the immunological experiments showed that HSP70C lead to enhanced vaccine potency, and suggested significant potential in the development of genetically engineered vaccines against HTNV.
authors: Department of Microbiology, Fourth Military Medical University, Xi'an, China.
authors: Linfeng
authors: L
authors: Cheng
authors: Department of Microbiology, Fourth Military Medical University, Xi'an, China.
authors: Lan
authors: L
authors: Yu
authors: Department of Microbiology, Fourth Military Medical University, Xi'an, China.
authors: Xingan
authors: X
authors: Wu
authors: Department of Microbiology, Fourth Military Medical University, Xi'an, China.
authors: Kai
authors: K
authors: Li
authors: Department of Microbiology, Fourth Military Medical University, Xi'an, China.
authors: Fang
authors: F
authors: Wang
authors: Department of Microbiology, Fourth Military Medical University, Xi'an, China.
authors: Liang
authors: L
authors: Zhang
authors: Department of Microbiology, Fourth Military Medical University, Xi'an, China.
authors: Wei
authors: W
authors: Ye
authors: Department of Microbiology, Fourth Military Medical University, Xi'an, China.
authors: Puyuan
authors: P
authors: Li
authors: Department of Microbiology, Fourth Military Medical University, Xi'an, China.
authors: Fanglin
authors: F
authors: Zhang
authors: Department of Microbiology, Fourth Military Medical University, Xi'an, China.
authors: Zhikai
authors: Z
authors: Xu
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000917:Antibody Formation / Q000276:immunology; D005260:Female; D050939:Gene Fusion / Q000235:genetics / Q000276:immunology; D005822:Genetic Vectors / Q000235:genetics / Q000276:immunology*; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000276:immunology*; D018063:Hantaan virus / Q000235:genetics / Q000276:immunology*; D007111:Immunity, Cellular / Q000235:genetics / Q000276:immunology*; D056724:Immunity, Humoral / Q000235:genetics / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology*; D014611:Vaccination / Q000379:methods; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D006023:Glycoproteins; D018840:HSP70 Heat-Shock Proteins; D019590:Nucleocapsid Proteins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D014765:Viral Vaccines