title: [Diagnostic implications of atypical squamous cells of unknown significance with abnormal DNA ploidy for early cervical lesions].
abstract: OBJECTIVE
  To investigate the clinical significance of atypical squamous cells of unknown significance (ASCUS) with abnormal DNA ploidy in the early diagnosis of cervical lesions.
  
  
  METHODS
  Eight thousand four hundred and forty-eight patients were included in this study and all had DNA quantitative analysis and cervical liquid-based cytology. Among 1041 cases with DNA aneuploidy and/or abnormal cervical liquid-based cytology and additional cervical biopsy, histological review was performed in 247 ASCUS cases with abnormal DNA ploidy.
  
  
  RESULTS
  (1) Among 8448 cases, 7877 were normal or benign, 426 were ASCUS, 45 were ASC-H, 55 were LSIL and 22 were HSIL by TBS diagnosis. The presence of 1-2 abnormal DNA ploidy cells was detected in 15.3% (65/426) of ASCUS, 11.1% (5/45) of ASC-H, 9.1% (5/55) of LSIL, and 0 (0/22) of HSIL. The presence of ≥ 3 abnormal DNA ploidy cells was detected in 39.0% (166/426) of ASCUS, 75.6% (34/45) of ASC-H, 76.4% (42/55) of LSIL, and 95.5% (21/22) of HSIL. (2) A total of 67 cases of CIN 2, CIN 3 or cancers were found in 247 patients with ASCUS by colposcopy biopsies, of which 13.9% (5/36) had 1-2 abnormal DNA ploidy cells, 45.5% (56/123) had ≥ 3 abnormal DNA ploidy cells and 6.8% (6/88) had normal DNA polidy. ASCUS with 1-2 abnormal DNA ploidy cells and with ≥ 3 abnormal DNA ploidy cells were compared. The difference was statistically significant (χ(2) = 11.79, P < 0.01). But the difference between ASCUS with 1-2 abnormal DNA ploidy cells and normal DNA ploidy had no statistical significance (P > 0.05).
  
  
  CONCLUSIONS
  ASCUS with ≥ 3 abnormal DNA ploidy cells has higher risk for developing CIN 2, CIN 3 or invasive carcinoma. The application of DNA quantitative analysis and cervical liquid-based cytology test can help in guiding clinical follow-up and treatment options in patients with ASCUS.
authors: Department of Pathology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China. E-mail: mjhdoctor@126.com.
authors: Jin-hong
authors: JH
authors: Mei
authors: Department of Pathology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors: Shan
authors: S
authors: Xu
authors: Department of Pathology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors: Yong-liang
authors: YL
authors: Han
authors: Department of Pathology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors: Yi
authors: Y
authors: Tu
authors: Department of Pathology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors: Yi-feng
authors: YF
authors: Xiong
authors: Department of Pathology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors: Yan-qing
authors: YQ
authors: Yu
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D000293:Adolescent; D000328:Adult; D000368:Aged; D000782:Aneuploidy; D002278:Carcinoma in Situ / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D003127:Colposcopy; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D006801:Humans; D008875:Middle Aged; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D055815:Young Adult
chemical_list: D004273:DNA, Neoplasm