title: Altered expression of podoplanin in keratocystic odontogenic tumours following decompression.
abstract: Marsupialisation or decompression is frequently performed as a conservative therapy for keratocystic odontogenic tumours (KCOTs). Positive podoplanin (PDPN) expression in the epithelium of KCOT has been previously reported and may be associated with neoplastic invasion. In the present study, changes in PDPN expression were observed in the epithelium of KCOTs following decompression. In total, 16 pairs of paraffin-embedded tissue specimens obtained at the time of decompression and at two-stage curettage or enucleation were collected and immunohistochemically examined using an antibody against PDPN. The intensity of PDPN staining was evaluated with a semi-quantitative detection method and statistically analysed. The immunohistochemical reactivity of PDPN was consistently markedly positive in 93.8% of KCOT samples prior to decompression. The positive staining was immunolocalised to the cell membrane and cytoplasm of cells in the basal layer and extended into the suprabasal layer for two to three cell layers. At the time of curettage, 2 of the 16 (12.5%) cases were completely negative, 11 of the 16 (68.8%) cases were locally positive and 3 of the 16 (18.7%) cases showed a 'linear staining' pattern, as the PDPN-positive cells were restricted to within the single basal layer. The expression level of PDPN was significantly decreased (P<0.05) and a significant loss or reduction of PDPN expression was observed in KCOTs following decompression. Larger sample groups are required to further verify this result.
authors: Institute of Stomatology, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
authors: Xiaomin
authors: X
authors: Zhang
authors: Institute of Stomatology, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
authors: Jing
authors: J
authors: Wang
authors: Institute of Stomatology, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
authors: Xu
authors: X
authors: Ding
authors: Institute of Stomatology, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
authors: Shuzhong
authors: S
authors: Xing
authors: Institute of Stomatology, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
authors: Wei
authors: W
authors: Zhang
authors: Division of Oral Pathology, The Shanghai Ninth People's Hospital, Shanghai 200011, P.R. China.
authors: Lizhen
authors: L
authors: Wang
authors: Institute of Stomatology, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
authors: Heming
authors: H
authors: Wu
authors: Institute of Stomatology, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
authors: Lin
authors: L
authors: Wang
keywords: decompression; immunohistochemistry; keratocystic odontogenic tumour; podoplanin