title: Expression of miR-29c, miR-93, and miR-429 as potential biomarkers for detection of early stage non-small lung cancer.
abstract: BACKGROUND
  Altered expression of miRNA expression contributes to human carcinogenesis. This study was designed to detect aberrant miRNA expressions as a potential biomarker for early detection and prognosis prediction of non-small cell lung cancer (NSCLC).
  
  
  METHODS
  miRNA array was used to profile differentially expressed miRNAs and Taqman-based quantitative RT-PCR assays were used to analyze levels of miR-29c, miR-93, and miR-429 expression in NSCLC tissue samples, corresponding normal tissue samples, and serum samples from 70 NSCLC patients as well as in serum samples from 48 healthy controls.
  
  
  RESULTS
  Levels of miR-29c and miR-93 expression were upregulated in NSCLC tissues, while serum levels of miR-29c were also upregulated, but levels of serum miR-429 were decreased in NSCLC. Moreover, the levels of miR-429 expression in NSCLC tissues were associated with those in serum samples. Receiver operating characteristic (ROC) curve analysis showed that at the optimal cut-off point, the areas under the ROC curve for serum levels of miR-29c and miR-429 were 0.723 and 0.727, respectively, levels which are higher than that of carcinoma embryonic antigen (0.534) in diagnosis of stage I NSCLC. In addition, serum levels of miR-429 were associated with poor overall survival of NSCLC patients. Both univariate and multivariate analyses showed that serum miR-429 level was an independent prognostic predictor for NSCLC.
  
  
  CONCLUSIONS
  The results of the current study suggest that detection of serum miR-29c and miR-429 expression should be further evaluated as a novel, non-invasive biomarker for early stage NSCLC.
authors: Department of Joint Immunogenomics Laboratory, Zhoushan Hospital, Zhoushan, Zhejiang, China.
authors: Wangyu
authors: W
authors: Zhu
authors: Department of Joint Immunogenomics Laboratory, Zhoushan Hospital, Zhoushan, Zhejiang, China.
authors: Jianying
authors: J
authors: He
authors: Department of Joint Immunogenomics Laboratory, Zhoushan Hospital, Zhoushan, Zhejiang, China.
authors: Dongdong
authors: D
authors: Chen
authors: Department of Cardio-Thoracic Surgery, Zhoushan Hospital, Zhoushan, Zhejiang, China.
authors: Bingjie
authors: B
authors: Zhang
authors: Department of Joint Immunogenomics Laboratory, Zhoushan Hospital, Zhoushan, Zhejiang, China.
authors: Liyun
authors: L
authors: Xu
authors: Department of Cardio-Thoracic Surgery, Zhoushan Hospital, Zhoushan, Zhejiang, China.
authors: Haijie
authors: H
authors: Ma
authors: Department of Cardio-Thoracic Surgery, Zhoushan Hospital, Zhoushan, Zhejiang, China.
authors: Xiaoguang
authors: X
authors: Liu
authors: Department of Joint Immunogenomics Laboratory, Zhoushan Hospital, Zhoushan, Zhejiang, China.
authors: Yongkui
authors: Y
authors: Zhang
authors: Department of Joint Immunogenomics Laboratory, Zhoushan Hospital, Zhoushan, Zhejiang, China.
authors: Hanbo
authors: H
authors: Le
mesh_terms: D000328:Adult; D000368:Aged; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D015331:Cohort Studies; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D011379:Prognosis; D012372:ROC Curve
chemical_list: D014408:Biomarkers, Tumor; C532045:MIRN29a microRNA, human; C546363:MIRN429 microRNA, human; C543350:MIRN93 microRNA, human; D035683:MicroRNAs