title: Preparation, characterization and cytotoxicity of schizophyllan/silver nanoparticle composite.
abstract: Silver nanoparticles (Ag-NPs) have been successfully prepared with a simple and "green" chemical reduction method. Triple helical schizophyllan (SPG) was used for the first time as reducing and stabilizing agents. The effect of temperature, silver nitrate/schizophyllan concentrations, pH of the reactions medium and the reaction time were investigated. The obtained schizophyllan/Ag-NP was characterized by UV-vis spectroscopy, TEM, DLS, X-ray diffraction, TGA, and ATR-FTIR. The results revealed that, Ag-NPs attached to SPG through a strong non-covalent interaction, leading to good dispersion of Ag-NPs with a diameter of 6 nm within the biopolymer matrix. By increasing the pH of the reaction medium, the triple helical structure of SPG was partially broken. The SPG/AgNP nanocomposite was non-toxic for mouse fibroblast line (NIH-3T3) and human keratinocyte cell line (HaCaT).
authors: Central European Institute of Technology (CEITEC), Brno University of Technology, Brno Czech Republic; Textile Research Division, National Research Center, Dokki, P.O. 12622, Giza 12522, Egypt. Electronic address: abdel-mohsen@ceitec.vutbr.cz.
authors: A M
authors: AM
authors: Abdel-Mohsen
authors: Institute of Organic Chemistry and Technology, Faculty of Chemical Technology, University of Pardubice, Czech Republic.
authors: Rasha M
authors: RM
authors: Abdel-Rahman
authors: Chemistry Department, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia; Textile Research Division, National Research Center, Dokki, P.O. 12622, Giza 12522, Egypt. Electronic address: m_gaballa@yahoo.com.
authors: Moustafa M G
authors: MM
authors: Fouda
authors: Central European Institute of Technology (CEITEC), Brno University of Technology, Brno Czech Republic.
authors: L
authors: L
authors: Vojtova
authors: Institute of Materials Chemistry, Brno University of Technology, Czech Republic.
authors: L
authors: L
authors: Uhrova
authors: Chemistry Department, Faculty of Science, Damanhour University, Damanhour, Egypt.
authors: A F
authors: AF
authors: Hassan
authors: Chemistry Department, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia.
authors: Salem S
authors: SS
authors: Al-Deyab
authors: Chemistry Department, Faculty of Science, Fayoum University, Fayoum, Egypt.
authors: Ibrahim E
authors: IE
authors: El-Shamy
authors: Central European Institute of Technology (CEITEC), Brno University of Technology, Brno Czech Republic; Institute of Materials Chemistry, Brno University of Technology, Czech Republic.
authors: J
authors: J
authors: Jancar
mesh_terms: D000818:Animals; D002460:Cell Line; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015603:Keratinocytes / Q000187:drug effects; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D051379:Mice; D046529:Microscopy, Electron, Transmission; D041681:NIH 3T3 Cells; D012834:Silver / Q000737:chemistry*; D012566:Sizofiran / Q000737:chemistry / Q000633:toxicity*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014961:X-Ray Diffraction
chemical_list: D012834:Silver; D012566:Sizofiran
keywords: Cytotoxicity; Green synthesis; Schizophyllan; Silver nanoparticles