title: Introducing MINA--The Molecularly Imprinted Nanoparticle Assay.
abstract: A new ELISA- (enzyme-linked immunosorbent assay)-like assay is demonstrated in which no elements of biological origin are used for molecular recognition or signaling. Composite imprinted nanoparticles that contain a catalytic core and which are synthesized by using a solid-phase approach can simultaneously act as recognition/signaling elements, and be used with minimal modifications to standard assay protocols. This assay provides a new route towards replacement of unstable biomolecules in immunoassays.
authors: Saint-Petersburg State, Chemical-Pharmaceutical Academy, Saint-Petersburg, Prof. Popov St. 14, 197376, Russian Federation.
authors: Roman V
authors: RV
authors: Shutov
authors: Antonio
authors: A
authors: Guerreiro
authors: Ewa
authors: E
authors: Moczko
authors: Isabel Perez
authors: IP
authors: de Vargas-Sansalvador
authors: Iva
authors: I
authors: Chianella
authors: Michael J
authors: MJ
authors: Whitcombe
authors: Sergey A
authors: SA
authors: Piletsky
mesh_terms: D002138:Calibration; D005290:Ferric Compounds / Q000737:chemistry; D007118:Immunoassay / Q000379:methods*; D007700:Kinetics; D018625:Microscopy, Atomic Force; D054802:Molecular Imprinting / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D009195:Peroxidase / Q000378:metabolism; D060327:Solid-Phase Synthesis Techniques; D014640:Vancomycin / Q000494:pharmacology
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide; D014640:Vancomycin; D009195:Peroxidase
keywords: core-shell materials; molecular imprinting; peroxidases; solid-phase synthesis