title: A fast and biocompatible living virus labeling method based on sialic acid-phenylboronic acid recognition system.
abstract: The sialic acid (SA)-phenylboronic acid (PBA) recognition system is of particular interest in the bioconjugation field, because it is simple, fast, efficient, and biocompatible. In this paper, we report a novel method for reversibly labeling living virus with quantum dots (QDs) by taking advantage of this SA-PBA recognition system. The QDs were initially modified with PBA (QDs-PBA) to target them to the surface of vesicular stomatitis virus (VSV), which has abundant with SA on its envelope. The QDs-PBA was of good monodispersity and strong fluorescence, and could be conjugated with VSV by simply incubating with native VSV for 10 min at 37 °C, producing QDs-VSV that was capable of being imaged at the single virion level. The labeling efficiency attained 83 ± 4.3 % (mean ± SD); meanwhile, the activity and recognition ability of the labeled virus were minimally affected. This method was simple, rapid, and reversible. This work promotes the virus labeling development to a new step. That is, native viruses can be reversibly labeled without any modification.
authors: School of Life Science, Beijing Institute of Technology, Beijing, 100081, China.
authors: Li-Li
authors: LL
authors: Huang
authors: Yong-Jie
authors: YJ
authors: Jin
authors: Dongxu
authors: D
authors: Zhao
authors: Chao
authors: C
authors: Yu
authors: Jian
authors: J
authors: Hao
authors: Hai-Yan
authors: HY
authors: Xie
mesh_terms: D001897:Boronic Acids / Q000737:chemistry*; D019158:N-Acetylneuraminic Acid / Q000737:chemistry*; D045663:Quantum Dots; D013194:Staining and Labeling / Q000379:methods*; D014780:Viruses / Q000737:chemistry*
chemical_list: D001897:Boronic Acids; D019158:N-Acetylneuraminic Acid; C010686:benzeneboronic acid