title: Protein corona fingerprinting predicts the cellular interaction of gold and silver nanoparticles.
abstract: Using quantitative models to predict the biological interactions of nanoparticles will accelerate the translation of nanotechnology. Here, we characterized the serum protein corona 'fingerprint' formed around a library of 105 surface-modified gold nanoparticles. Applying a bioinformatics-inspired approach, we developed a multivariate model that uses the protein corona fingerprint to predict cell association 50% more accurately than a model that uses parameters describing nanoparticle size, aggregation state, and surface charge. Our model implicates a set of hyaluronan-binding proteins as mediators of nanoparticle-cell interactions. This study establishes a framework for developing a comprehensive database of protein corona fingerprints and biological responses for multiple nanoparticle types. Such a database can be used to develop quantitative relationships that predict the biological responses to nanoparticles and will aid in uncovering the fundamental mechanisms of nano-bio interactions.
authors: Institute of Biomaterials and Biomedical Engineering, ‡Banting and Best Department of Medical Research, §Donnelly Centre for Cellular and Biomolecular Research, ⊥Department of Chemical Engineering, ∥Department of Chemistry, #Department of Materials Science and Engineering, University of Toronto , Toronto, Ontario, Canada M5S 3G9.
authors: Carl D
authors: CD
authors: Walkey
authors: Jonathan B
authors: JB
authors: Olsen
authors: Fayi
authors: F
authors: Song
authors: Rong
authors: R
authors: Liu
authors: Hongbo
authors: H
authors: Guo
authors: D Wesley H
authors: DW
authors: Olsen
authors: Yoram
authors: Y
authors: Cohen
authors: Andrew
authors: A
authors: Emili
authors: Warren C W
authors: WC
authors: Chan
mesh_terms: D001798:Blood Proteins / Q000378:metabolism*; D002460:Cell Line; D006046:Gold / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D053768:Metal Nanoparticles; D036103:Nanotechnology; D010316:Particle Size; D011485:Protein Binding; D012834:Silver / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D001798:Blood Proteins; D012834:Silver; D006046:Gold