title: Deposition and transport of Pseudomonas aeruginosa in porous media: lab-scale experiments and model analysis.
abstract: In this study, the deposition and transport of Pseudomonas aeruginosa on sandy porous materials have been investigated under static and dynamic flow conditions. For the static experiments, both equilibrium and kinetic batch tests were performed at a 1:3 and 3:1 soil:solution ratio. The batch data were analysed to quantify the deposition parameters under static conditions. Column tests were performed for dynamic flow experiments with KCl solution and bacteria suspended in (1) deionized water, (2) mineral salt medium (MSM) and (3) surfactant + MSM. The equilibrium distribution coefficient (K(d)) was larger at a 1:3 (2.43 mL g(-1)) than that at a 3:1 (0.28 mL g(-1)) soil:solution ratio. Kinetic batch experiments showed that the reversible deposition rate coefficient (k(att)) and the release rate coefficient (k(det)) at a soil:solution ratio of 3:1 were larger than those at a 1:3 ratio. Column experiments showed that an increase in ionic strength resulted in a decrease in peak concentration of bacteria, mass recovery and tailing of the bacterial breakthrough curve (BTC) and that the presence of surfactant enhanced the movement of bacteria through quartz sand, giving increased mass recovery and tailing. Deposition parameters under dynamic condition were determined by fitting BTCs to four different transport models, (1) kinetic reversible, (2) two-site, (3) kinetic irreversible and (4) kinetic reversible and irreversible models. Among these models, Model 4 was more suitable than the others since it includes the irreversible sorption term directly related to the mass loss of bacteria observed in the column experiment. Applicability of the parameters obtained from the batch experiments to simulate the column breakthrough data is evaluated.
authors: Center for Water Resource Cycle Research, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea.
authors: Kyu-Sang
authors: KS
authors: Kwon
authors: Environmental Biocolloid Engineering Laboratory, Program in Rural System Engineering, Seoul National University, Seoul 151-742, Republic of Korea.
authors: Song-Bae
authors: SB
authors: Kim
authors: Environmental Biocolloid Engineering Laboratory, Program in Rural System Engineering, Seoul National University, Seoul 151-742, Republic of Korea.
authors: Nag-Choul
authors: NC
authors: Choi
authors: Department of Earth and Environmental Sciences, Korea University, Seoul 136-701, Republic of Korea.
authors: Dong-Ju
authors: DJ
authors: Kim
authors: Department of Earth and Environmental Sciences, Korea University, Seoul 136-701, Republic of Korea.
authors: Soonjae
authors: S
authors: Lee
authors: Department of Earth and Environmental Sciences, Korea University, Seoul 136-701, Republic of Korea.
authors: Sang-Hyup
authors: SH
authors: Lee
authors: Center for Water Resource Cycle Research, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea.
authors: Jae-Woo
authors: JW
authors: Choi
mesh_terms: D001422:Bacterial Adhesion; D007700:Kinetics; D008954:Models, Biological; D016062:Porosity; D011189:Potassium Chloride / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000166:cytology*; D012822:Silicon Dioxide / Q000737:chemistry; D012988:Soil Microbiology; D013501:Surface-Active Agents / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D013501:Surface-Active Agents; D014867:Water; D011189:Potassium Chloride; D012822:Silicon Dioxide