title: Preparation and differentiation of mesenchymal stem cells from ears of adult mice.
abstract: External murine ears collected postmortem, as well as ear punches obtained during standard marking of live animals, are the source of mesenchymal stem cells, termed ear mesenchymal stem cells (EMSC). These cells provide an easily obtainable, primary culture model system for the study of lineage commitment and differentiation. EMSC are capable of differentiating into adipocytes, osteocytes, chondrocytes, and contractile myocytes. Facile adipogenic differentiation of EMSC provides an excellent model for the study of adipogenesis. In this chapter, methods for isolation, culture, and differentiation of EMSC are described.
authors: Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland. Electronic address: b.kozak@pan.olsztyn.pl.
authors: Barbara
authors: B
authors: Gawronska-Kozak
mesh_terms: D017667:Adipocytes / Q000166:cytology; D050156:Adipogenesis; D000818:Animals; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology; D015925:Cryopreservation / Q000379:methods; D004423:Ear / Q000033:anatomy & histology; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice
keywords: Adipogenesis; Chondrogenesis; Differentiation; EMSC; Mesenchymal stem cells; Myogenesis; Osteogenesis