title: Purification of radiolabeled RNA products using denaturing gel electrophoresis.
abstract: This unit discusses a basic method for purification of radiolabeled RNAs using denaturing polyacrylamide gel electrophoresis. The method consists of a number of experimental procedures, including total RNA preparation from yeast cells, isolation of a specific RNA from total yeast RNA, RNA 3'-terminal labeling using nucleotide (5' [(32) P]pCp) addition (via ligation), denaturing (8 M urea) polyacrylamide gel electrophoresis, and RNA extraction from the gel slice. Key points for achieving good electrophoretic separation of RNA are also discussed.
authors: Department of Biochemistry and Biophysics, Center for RNA Biology, University of Rochester Medical Center, Rochester, New York.
authors: Hironori
authors: H
authors: Adachi
authors: Yi-Tao
authors: YT
authors: Yu
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D007553:Isotope Labeling / Q000379:methods*; D012313:RNA / Q000737:chemistry*; D015003:Yeasts / Q000235:genetics*
chemical_list: D012313:RNA
keywords: RNA molecular biology; RNA purification; denaturing polyacrylamide electrophoresis (PAGE); radiolabeled RNAs; yeast cells