title: Constructing STR multiplexes for individual identification of Hungarian red deer.
abstract: Red deer is the most valuable game of the fauna in Hungary, and there is a strong need for genetic identification of individuals. For this purpose, 10 tetranucleotide STR markers were developed and amplified in two 5-plex systems. The study presented here includes the flanking region sequence analysis and the allele nomenclature of the 10 loci as well as the PCR optimization of the DeerPlex I and II. LD pairwise tests and cross-species similarity analyses showed the 10 loci to be independently inherited. Considerable levels of genetic differences between two subpopulations were recorded, and F(ST) was 0.034 using AMOVA. The average probability of identity (PI(ave)) was at the value of 2.6736 × 10(-15). This low value for PI(ave) nearly eliminates false identification. An illegal hunting case solved by DeerPlex is described herein. The calculated likelihood ratio (LR) illustrates the potential of the 10 red deer microsatellite markers for forensic investigations.
authors: Department of Genetics, Eötvös Loránd University, Pázmány Péter s. 1/c, 1117, Budapest, Hungary; Institute of Molecular Genetics, Agricultural Biotechnology Center, Szent-Györgyi Albert u. 4, 2100, Gödöllő, Hungary; Department of Genetics, Institute for Forensic Sciences, Mosonyi u. 9, 1087, Budapest, Hungary.
authors: Zoltan
authors: Z
authors: Szabolcsi
authors: Balazs
authors: B
authors: Egyed
authors: Petra
authors: P
authors: Zenke
authors: Zsolt
authors: Z
authors: Padar
authors: Adrienn
authors: A
authors: Borsy
authors: Viktor
authors: V
authors: Steger
authors: Erzsebet
authors: E
authors: Pasztor
authors: Sandor
authors: S
authors: Csanyi
authors: Zsuzsanna
authors: Z
authors: Buzas
authors: Laszlo
authors: L
authors: Orosz
mesh_terms: D000483:Alleles; D000818:Animals; D003247:Conservation of Natural Resources; D003670:Deer / Q000235:genetics*; D005838:Genotype; D006814:Hungary; D015810:Linkage Disequilibrium; D018895:Microsatellite Repeats; D060885:Multiplex Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013045:Species Specificity
keywords: Cervus elaphus hippelaphus; deer genetic identification; forensic science; population structure; red deer; tetranucleotide microsatellite; wildlife forensic genetics