title: Morphological and molecular characterization of lymnaeid snails and their potential role in transmission of Fasciola spp. in Vietnam.
abstract: Freshwater snails of the family Lymnaeidae play an important role in the transmission of fascioliasis worldwide. In Vietnam, 2 common lymnaeid species, Lymnaea swinhoei and Lymnaea viridis, can be recognized on the basis of morphology, and a third species, Lymnaea sp., is known to exist. Recent studies have raised controversy about their role in transmission of Fasciola spp. because of confusion in identification of the snail hosts. The aim of this study is, therefore, to clarify the identities of lymnaeid snails in Vietnam by a combination of morphological and molecular approaches. The molecular analyses using the second internal transcribed spacer (ITS2) of the nuclear ribosomal DNA clearly showed that lymnaeids in Vietnam include 3 species, Austropeplea viridis (morphologically identified as L. viridis), Radix auricularia (morphologically identified as L. swinhoei) and Radix rubiginosa (morphologically identified as Lymnaea sp.). R. rubiginosa is a new record for Vietnam. Among them, only A. viridis was found to be infected with Fasciola spp. These results provide a new insight into lymnaeid snails in Vietnam. Identification of lymnaeid snails in Vietnam and their role in the liver fluke transmission should be further investigated.
authors: Department of Parasitology, Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Vietnam. ; Research Unit in Parasitology and Parasitic Diseases, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, Boulevard de Colonter 20, 4000 Liège, Belgium.
authors: Bui Thi
authors: BT
authors: Dung
authors: Department of Parasitology, Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Vietnam.
authors: Pham Ngoc
authors: PN
authors: Doanh
authors: Department of Molecular Systematic and Conservation Genetic, Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Vietnam.
authors: Dang Tat
authors: DT
authors: The
authors: Department of Molecular Systematic and Conservation Genetic, Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Vietnam.
authors: Ho Thi
authors: HT
authors: Loan
authors: Research Unit in Parasitology and Parasitic Diseases, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, Boulevard de Colonter 20, 4000 Liège, Belgium.
authors: Bertrand
authors: B
authors: Losson
authors: Research Unit in Parasitology and Parasitic Diseases, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, Boulevard de Colonter 20, 4000 Liège, Belgium.
authors: Yannick
authors: Y
authors: Caron
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005209:Fasciola / Q000302:isolation & purification*; D008195:Lymnaea / Q000033:anatomy & histology* / Q000235:genetics / Q000469:parasitology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014744:Vietnam
chemical_list: D021903:DNA, Ribosomal Spacer
keywords: Austropeplea viridis; Fasciola; Radix auricularia; Radix rubiginosa; Vietnam