title: Lycopene treatment prevents hematological, reproductive and histopathological damage induced by acute zearalenone administration in male Swiss mice.
abstract: Zearalenone (ZEA) is a mycotoxin commonly found as a contaminant in cereals. ZEA toxicity targets mainly the reproductive system, and oxidative stress plays an etiological role in its toxic effects. Therefore, the present study aimed to investigate the effect of lycopene, a potent carotenoid antioxidant, on markers of oxidative stress in liver, kidney and testes, and on reproductive, hematological and histopathological parameters after ZEA administration. Adult Swiss albino male mice received lycopene (20mg/kg, p.o.) for ten days before a single oral administration of ZEA (40mg/kg, p.o.), and 48h thereafter tissues (liver, kidney, testes and blood) were collected for biochemical, hematological and histological analyses. Lycopene prevented ZEA-induced changes in hematological parameters (increased number of leukocytes, segmented neutrophils, sticks, eosinophils and monocytes and decreased number of red blood cells (RBC), number of lymphocytes and platelets). Moreover, lycopene prevented the reduction in the number and motility of spermatozoa and the testicular tissue damage induced by ZEA. In addition, lycopene prevented the decrease in glutathione-S-transferase activity in kidney and testes and increased glutathione-S-transferase activity per se in the liver, kidneys and testes as well as superoxide dismutase activity in the liver. In summary, lycopene was able to prevent ZEA-induced acute toxic effects in male mice, suggesting that this antioxidant carotenoid may represent a promising prophylactic strategy against ZEA toxicity.
authors: Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil.
authors: Silvana Peterini
authors: SP
authors: Boeira
authors: Universidade Federal do Pampa, Campus Itaqui, 97650-000 Itaqui, RS, Brazil.
authors: Carlos Borges
authors: CB
authors: Filho
authors: Universidade Federal do Pampa, Campus Itaqui, 97650-000 Itaqui, RS, Brazil.
authors: Lucian
authors: L
authors: Del'Fabbro
authors: Universidade Regional Integrada do Alto Uruguai e das Missões, Campus de Erechim, 99700-000 Erechim, RS, Brazil.
authors: Silvane Souza
authors: SS
authors: Roman
authors: Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil; Departamento de Educação Física e Desportos, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil.
authors: Luiz Fernando Freire
authors: LF
authors: Royes
authors: Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil; Departamento de Neuropsiquiatria, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil.
authors: Michele Rechia
authors: MR
authors: Fighera
authors: Universidade Federal do Pampa, Campus Itaqui, 97650-000 Itaqui, RS, Brazil; Programa de Pós-Graduação em Bioquímica, Universidade Federal do Pampa, 97500-970 Uruguaiana, RS, Brazil.
authors: Cristiano Ricardo
authors: CR
authors: Jessé
authors: Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil.
authors: Mauro Schneider
authors: MS
authors: Oliveira
authors: Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil; Departamento de Tecnologia e Ciência dos Alimentos, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil. Electronic address: furian.anaflavia@gmail.com.
authors: Ana Flávia
authors: AF
authors: Furian
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000627:therapeutic use*; D001772:Blood Cell Count; D002338:Carotenoids / Q000008:administration & dosage / Q000627:therapeutic use*; D064419:Chemically-Induced Disorders / Q000097:blood / Q000473:pathology / Q000517:prevention & control; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D000077276:Lycopene; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D012098:Reproduction / Q000187:drug effects*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000473:pathology; D013737:Testis / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D015025:Zearalenone / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D002338:Carotenoids; D015025:Zearalenone; D000077276:Lycopene
keywords: Carotenoid; GST; Lycopene; Mycotoxin; Spermatozoa; Testes