title: Contributions to the phytochemical study of Bidens tripartitae herba from Romania. I. Tannins.
abstract: OBJECTIVE
  To analyze qualitatively and quantitatively tannins in the native plant, collected during the whole vegetation period from different areas of the country, and in its different organs (flower, stem, leaf).
  
  
  METHODS
  For quantitative analysis, the plant product was extracted by repeated maceration (3 days) with 80% methanol. Proanthocyanidins in the extract were quantified by spectrophotometric methods.
  
  
  RESULTS
  condensed tannins were present while hydrolyzed tannins were absent. Chromatographic analysis showed that tannins spectrum is similar in all plant organs and in plants collected at different times throughout the vegetation period. The differences are only quantitative. The maximum amount of tannins was found during the flowering stage (10.32%).
  
  
  CONCLUSIONS
  In terms of tannin content, flowering is the best time to collect. However, collection throughout the whole vegetation period is acceptable.
authors: Faculty of Medical Bioengineering, University of Medicine and Pharmacy Grigore T. Popa, Iasi.
authors: M
authors: M
authors: Zagnat
authors: Faculty of Medical Bioengineering, University of Medicine and Pharmacy Grigore T. Popa, Iasi.
authors: Corina
authors: C
authors: Cheptea
authors: Faculty of Pharmacy, University of Medicine and Pharmacy Grigore T. Popa, Iasi.
authors: A F
authors: AF
authors: Spac
mesh_terms: D031176:Bidens; D002845:Chromatography; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry*; D012383:Romania; D013634:Tannins / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D013634:Tannins