title: The Arabidopsis thaliana cysteine-rich receptor-like kinases CRK6 and CRK7 protect against apoplastic oxidative stress.
abstract: Receptor-like kinases are important regulators of many different processes in plants. Despite their large number only a few have been functionally characterized. One of the largest subgroups of receptor-like kinases in Arabidopsis is the cysteine-rich receptor like kinases (CRKs). High sequence similarity among the CRKs has been suggested as major cause for functional redundancy. The genomic localization of CRK genes in back-to-back repeats has made their characterization through mutant analysis unpractical. Expression profiling has linked the CRKs with reactive oxygen species, important signaling molecules in plants. Here we have investigated the role of two CRKs, CRK6 and CRK7, and analyzed their role in extracellular ROS signaling. CRK6 and CRK7 are active protein kinases with differential preference for divalent cations. Our results suggest that CRK7 is involved in mediating the responses to extracellular but not chloroplastic ROS production.
authors: Division of Plant Biology, Department of Biosciences, University of Helsinki, FI-00014 Helsinki, Finland. Electronic address: niina.idanheimo@helsinki.fi.
authors: Niina
authors: N
authors: Idänheimo
authors: Division of Plant Biology, Department of Biosciences, University of Helsinki, FI-00014 Helsinki, Finland. Electronic address: adrien.gauthier@helsinki.fi.
authors: Adrien
authors: A
authors: Gauthier
authors: Division of Plant Biology, Department of Biosciences, University of Helsinki, FI-00014 Helsinki, Finland. Electronic address: jarkko.salojarvi@helsinki.fi.
authors: Jarkko
authors: J
authors: Salojärvi
authors: Division of Plant Biology, Department of Biosciences, University of Helsinki, FI-00014 Helsinki, Finland; Institute of Biotechnology, University of Helsinki, FI-00014 Helsinki, Finland. Electronic address: riccardo.siligato@helsinki.fi.
authors: Riccardo
authors: R
authors: Siligato
authors: Division of Plant Biology, Department of Biosciences, University of Helsinki, FI-00014 Helsinki, Finland; Institute of Technology, University of Tartu, Tartu 50411, Estonia. Electronic address: mikael.brosche@helsinki.fi.
authors: Mikael
authors: M
authors: Brosché
authors: Institute of Technology, University of Tartu, Tartu 50411, Estonia. Electronic address: hannes.kollist@ut.ee.
authors: Hannes
authors: H
authors: Kollist
authors: Division of Plant Biology, Department of Biosciences, University of Helsinki, FI-00014 Helsinki, Finland; Institute of Biotechnology, University of Helsinki, FI-00014 Helsinki, Finland. Electronic address: aripekka.mahonen@helsinki.fi.
authors: Ari Pekka
authors: AP
authors: Mähönen
authors: Division of Plant Biology, Department of Biosciences, University of Helsinki, FI-00014 Helsinki, Finland. Electronic address: jaakko.kangasjarvi@helsinki.fi.
authors: Jaakko
authors: J
authors: Kangasjärvi
authors: Division of Plant Biology, Department of Biosciences, University of Helsinki, FI-00014 Helsinki, Finland. Electronic address: michael.wrzaczek@helsinki.fi.
authors: Michael
authors: M
authors: Wrzaczek
mesh_terms: D017360:Arabidopsis / Q000201:enzymology / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D017382:Reactive Oxygen Species; C588276:CRK6 protein, Arabidopsis; C588277:CRK7 protein, Arabidopsis; D018844:Cyclin-Dependent Kinases
keywords: Arabidopsis thaliana; CRK; Oxidative stress; RLK; ROS signaling; Redundancy