title: The effects of x-ray radiation on the eye development of zebrafish.
abstract: The toxic effects of x-ray radiation on eye development was measured using zebrafish as a model organism. Zebrafish embryos at 8 h post-fertilization (hpf) were irradiated using X-rays at doses of 1, 2, 4, and 8 Gy. At 24 and 48 hpf, x-ray radiation induced a significant increase in reactive oxygen species (ROS) content and cell apoptotic signals. Both of these increases were dose dependent and there were significant positive relationships between them at 24 hpf. At 48 and 72 hpf, the increase of ROS concentration can be eliminated by increasing activities of superoxide dismutase and catalase. Although the ROS generated by x-ray radiation caused a significant increase in cell apoptosis at 24 and 48 hpf, the cellular layers of the retina and lens formation in the irradiated groups were not significantly disrupted at 144 hpf compared with the control group, with the exception of a heterogeneous distribution of the cells in inner nuclear cell layer and a significant decrease in the diameters of whole eyes after 8 Gy irradiation. X-Ray radiation at later stages of gastrulation may not cause distinct optic complications; however, there is still a risk of microophthalmia at high doses of irradiation.
authors: Department of Medical Physics, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China Key Laboratory of Heavy Ion Radiation Biology and Medicine, Chinese Academy of Sciences, Lanzhou, China Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province, Lanzhou, China.
authors: R
authors: R
authors: Zhou
authors: Department of Medical Physics, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China Key Laboratory of Heavy Ion Radiation Biology and Medicine, Chinese Academy of Sciences, Lanzhou, China Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province, Lanzhou, China.
authors: J
authors: J
authors: Si
authors: Department of Medical Physics, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China Key Laboratory of Heavy Ion Radiation Biology and Medicine, Chinese Academy of Sciences, Lanzhou, China Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province, Lanzhou, China zhangh@impcas.ac.cn.
authors: H
authors: H
authors: Zhang
authors: School/Hospital of Stomatology, Lanzhou University, Lanzhou, China.
authors: Z
authors: Z
authors: Wang
authors: College of Life Sciences, Northwest Normal University, Lanzhou, China.
authors: J
authors: J
authors: Li
authors: Department of Medical Physics, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China Key Laboratory of Heavy Ion Radiation Biology and Medicine, Chinese Academy of Sciences, Lanzhou, China Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province, Lanzhou, China.
authors: X
authors: X
authors: Zhou
authors: Department of Medical Physics, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China Key Laboratory of Heavy Ion Radiation Biology and Medicine, Chinese Academy of Sciences, Lanzhou, China Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province, Lanzhou, China.
authors: L
authors: L
authors: Gan
authors: Department of Medical Physics, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China Key Laboratory of Heavy Ion Radiation Biology and Medicine, Chinese Academy of Sciences, Lanzhou, China Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province, Lanzhou, China.
authors: Y
authors: Y
authors: Liu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005123:Eye / Q000196:embryology / Q000378:metabolism / Q000528:radiation effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013997:Time Factors; D014965:X-Rays / Q000009:adverse effects*; D015027:Zebrafish / Q000196:embryology* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species
keywords: X-Ray; cell apoptosis; eye development; microophthalmia; reactive oxygen species; zebrafish