title: Synthesis of biocontrol macromolecules by derivative of chitosan with surfactin and antifungal evaluation.
abstract: A derivative of chitosan was prepared with chitosan and β-cyclodextrins, which was synthesized by the immobilization reaction, as a carrier to adsorb surfactin produced from Bacillus amyloliquefaciens and got biological macromolecules. The antifungal activity against three sapstain fungi by a combination of macromolecules was tested. The results showed that the macromolecules inhibited the mycelium growth of sapstain fungi Lasiodiplodia rubropurpurea, L. crassispora, and L. theobromae by about 73.22%, 76.72%, and 70.22%, respectively. The macromolecules were relatively thermally stable with more than 50% of the antifungal activity even after being held at 121°C for 30 min. Meanwhile, the activity of the macromolecules remained more than 55% at a pH value ranging from 4 to 12. The macromolecules were resistant to hydrolysis by most protein-denaturing detergents and other enzymes. The results indicated the macromolecules might provide an alternative bioresource for the bio-control of sapstain.
authors: The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, Jiangsu Normal University, Xuzhou, Jiangsu 221116, PR China.
authors: Bo
authors: B
authors: Yuan
authors: The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, Jiangsu Normal University, Xuzhou, Jiangsu 221116, PR China.
authors: Pei-Yuan
authors: PY
authors: Xu
authors: The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, Jiangsu Normal University, Xuzhou, Jiangsu 221116, PR China.
authors: Yue-Ji
authors: YJ
authors: Zhang
authors: The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, Jiangsu Normal University, Xuzhou, Jiangsu 221116, PR China.
authors: Pei-Pei
authors: PP
authors: Wang
authors: The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, Jiangsu Normal University, Xuzhou, Jiangsu 221116, PR China.
authors: Hong
authors: H
authors: Yu
authors: The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, Jiangsu Normal University, Xuzhou, Jiangsu 221116, PR China. Electronic address: jhjiang@jsnu.edu.cn.
authors: Ji-Hong
authors: JH
authors: Jiang
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry* / Q000494:pharmacology*; D001407:Bacillus / Q000378:metabolism; D048271:Chitosan / Q000737:chemistry*; D005658:Fungi / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D046911:Macromolecular Substances / Q000737:chemistry* / Q000494:pharmacology*; D025282:Mycelium / Q000187:drug effects; D013696:Temperature; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D000935:Antifungal Agents; D046911:Macromolecular Substances; D047392:beta-Cyclodextrins; D048271:Chitosan
keywords: Anti-sapstain fungi; Biological macromolecules; Synthesis