title: Overexpression of NDRG2 can inhibit neuroblastoma cell proliferation through negative regulation by CYR61.
abstract: Several recent studies have showed that the n-myc downstream regulated gene 2 (NDRG2) is a new tumor suppressor gene, and that it plays an important role in tumor suppression in several cancers or cancer cell lines. However, few studies focused on its function in neuroblastoma cells. In the present investigation, we demonstrated that NDRG2 overexpression inhibited their proliferation. Using a cDNA microarray, we found that overexpression of NDRG2 inhibited the expression of cysteine-rich protein 61 (CYR61), a proliferation related gene. From our research, CYR61 may partially hinder NDRG2-mediated inhibition of cell proliferation. Overexpression of NDRG2 resulted in accumulation of cells in the G1 phase, which was accompanied by upregulation of p21 and p27 and downregulation of CDK4 and cyclin D1. Taken together, these data indicate that NDRG2 inhibits the proliferation of neuroblastoma cells partially through suppression of CYR61. Our findings offer novel insights into the physiological roles of NDRG2 in neuroblastoma cell proliferation, and NDRG2 may prove to be effective candidate for the treatment of children with neuroblastoma.
authors: Department of Neurosurgery and Institute for Functional Brain Disorders, Tangdu Hospital, Xi'an, China E-mail : sky1010222@163.com.
authors: Zhi-Guo
authors: ZG
authors: Zhang
authors: Gang
authors: G
authors: Li
authors: Da-Yun
authors: DY
authors: Feng
authors: Jian
authors: J
authors: Zhang
authors: Jing
authors: J
authors: Zhang
authors: Huai-Zhou
authors: HZ
authors: Qin
authors: Lian-Ting
authors: LT
authors: Ma
authors: Guo-Dong
authors: GD
authors: Gao
authors: Lin
authors: L
authors: Wu
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000235:genetics; D051358:Cyclin-Dependent Kinase 4 / Q000235:genetics; D055515:Cysteine-Rich Protein 61 / Q000235:genetics*; D018076:DNA, Complementary; D015536:Down-Regulation; D059585:G1 Phase Cell Cycle Checkpoints; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016086:Lentivirus; D009447:Neuroblastoma / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D010957:Plasmids; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D014162:Transfection; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D015854:Up-Regulation
chemical_list: C501848:CCN1 protein, human; D055515:Cysteine-Rich Protein 61; D018076:DNA, Complementary; C466228:NDRG2 protein, human; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins; C413374:p27 antigen; D019938:Cyclin D1; C495900:CDK4 protein, human; D051358:Cyclin-Dependent Kinase 4