title: Characterization and antioxidant properties of six Algerian propolis extracts: ethyl acetate extracts inhibit myeloperoxidase activity.
abstract: Because propolis contains many types of antioxidant compounds such as polyphenols and flavonoids, it can be useful in preventing oxidative damages. Ethyl acetate extracts of propolis from several Algerian regions show high activity by scavenging free radicals, preventing lipid peroxidation and inhibiting myeloperoxidase (MPO). By fractioning and assaying ethyl acetate extracts, it was observed that both polyphenols and flavonoids contribute to these activities. A correlation was observed between the polyphenol content and the MPO inhibition. However, it seems that kaempferol, a flavonoid, contributes mainly to the MPO inhibition. This molecule is in a high amount in the ethyl acetate extract and demonstrates the best efficiency towards the enzyme with an inhibiting concentration at 50% of 4 ± 2 µM.
authors: Laboratory of Beneficial Microorganisms, Functional Food and Health (LMBAFS), Faculty of Natural Sciences and Life, University of Abdelhamid Ibn Badis, Mostaganem 27000, Algeria. yasminaboufadi@yahoo.fr.
authors: Yasmina Mokhtaria
authors: YM
authors: Boufadi
authors: Laboratory of Pharmaceutical Chemistry, Faculty of Pharmacy, Universite Libre de Bruxelles, Brussels 1050, Belgium. jal.sub@hotmail.com.
authors: Jalal
authors: J
authors: Soubhye
authors: Laboratory of Beneficial Microorganisms, Functional Food and Health (LMBAFS), Faculty of Natural Sciences and Life, University of Abdelhamid Ibn Badis, Mostaganem 27000, Algeria. Ardz22003@yahoo.fr.
authors: Ali
authors: A
authors: Riazi
authors: Laboratory of Experimental Medicine, CHU Charleroi, A. Vésale Hospital, Universite Libre de Bruxelles, Montigny-le-Tilleul 6110, Belgium. Alexandre.ROUSSEAU@chu-charleroi.be.
authors: Alexandre
authors: A
authors: Rousseau
authors: Laboratory of Experimental Medicine, CHU Charleroi, A. Vésale Hospital, Universite Libre de Bruxelles, Montigny-le-Tilleul 6110, Belgium. michel.vanhaeverbeek@ulb.ac.be.
authors: Michel
authors: M
authors: Vanhaeverbeek
authors: Laboratory of Pharmaceutical Chemistry, Faculty of Pharmacy, Universite Libre de Bruxelles, Brussels 1050, Belgium. jneve@ulb.ac.be.
authors: Jean
authors: J
authors: Nève
authors: Laboratory of Experimental Medicine, CHU Charleroi, A. Vésale Hospital, Universite Libre de Bruxelles, Montigny-le-Tilleul 6110, Belgium. karim.zouaoui@chu-charleroi.be.
authors: Karim Zouaoui
authors: KZ
authors: Boudjeltia
authors: Laboratory of Pharmaceutical Chemistry, Faculty of Pharmacy, Universite Libre de Bruxelles, Brussels 1050, Belgium. pvantwer@ulb.ac.be.
authors: Pierre
authors: P
authors: Van Antwerpen
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000737:chemistry; D004789:Enzyme Activation / Q000187:drug effects; D005419:Flavonoids / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D015227:Lipid Peroxidation / Q000187:drug effects; D059625:Liquid-Liquid Extraction; D010084:Oxidation-Reduction / Q000187:drug effects; D009195:Peroxidase / Q000037:antagonists & inhibitors; D059808:Polyphenols / Q000737:chemistry; D011429:Propolis / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D016166:Free Radical Scavengers; D059808:Polyphenols; D011429:Propolis; D009195:Peroxidase; D001205:Ascorbic Acid