title: Substrate-mediated, high-efficiency siRNA electroporation.
abstract: Here, we outline a method for surface-mediated transfection of small interfering RNA (siRNA) using electroporation. Cells are cultured on an electrode carrying siRNA. After the cells are adhered on the electrode surface, an electric pulse is applied to release siRNA from the surface and transfect it into the cells. A siRNA electroporation microarray is also presented for high-throughput screening of the functions of many siRNAs.
authors: Department of Diabetes, Endocrinology and Nutrition, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
authors: Hiroyuki
authors: H
authors: Fujimoto
authors: Hiroo
authors: H
authors: Iwata
mesh_terms: D001483:Base Sequence; D004566:Electrodes; D018274:Electroporation / Q000295:instrumentation / Q000379:methods*; D057809:HEK293 Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism*; D046888:Tissue Array Analysis / Q000295:instrumentation / Q000379:methods*; D014162:Transfection / Q000295:instrumentation / Q000379:methods*
chemical_list: D034741:RNA, Small Interfering