title: Equine arteritis virus gP5 protein induces apoptosis in cultured insect cells.
abstract: Equine Arteritis Virus (EAV) has been shown to induce apoptosis in vitro but the induction of this mechanism has not been previously associated with any viral gene product. In this work, we found a cytotoxicity effect of the EAV gP5 protein on baculovirus-insect cells and a low yield of protein recovery. Besides, different morphological features by electron transmission microscopy, DNA fragmentation in agarose gel, TUNEL analysis and caspase 3 activity were found. All these findings indicate that the EAV gP5 protein induces apoptosis in insect cells.
authors: Virology, Faculty of Veterinary Sciences, National University of La Plata, La Plata, Argentina; Members of CONICET (CCT-La Plata), Argentina.
authors: Germán Ernesto
authors: GE
authors: Metz
authors: Virology, Faculty of Veterinary Sciences, National University of La Plata, La Plata, Argentina; Members of CONICET (CCT-La Plata), Argentina.
authors: María Soledad
authors: MS
authors: Serena
authors: Members of CONICET (CCT-La Plata), Argentina; IGEVET-CCT-La Plata, Argentina.
authors: María Mercedes
authors: MM
authors: Abeyá
authors: Virology, Faculty of Veterinary Sciences, National University of La Plata, La Plata, Argentina.
authors: Andrea Belén
authors: AB
authors: Dulbecco
authors: Laboratorio de Patología Especial, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Argentina.
authors: Adriana
authors: A
authors: Massone
authors: Members of CONICET (CCT-La Plata), Argentina; IGEVET-CCT-La Plata, Argentina.
authors: Silvina
authors: S
authors: Díaz
authors: Virology, Faculty of Veterinary Sciences, National University of La Plata, La Plata, Argentina; Members of CONICET (CCT-La Plata), Argentina; IGEVET-CCT-La Plata, Argentina. Electronic address: mariagabrielaecheverria@yahoo.com.ar.
authors: María Gabriela
authors: MG
authors: Echeverría
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis; D016367:Baculoviridae / Q000235:genetics; D007240:Equartevirus / Q000472:pathogenicity / Q000502:physiology*; D015870:Gene Expression; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D061987:Sf9 Cells; D018411:Spodoptera; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D000956:Antigens, Viral; C550278:GP5 protein, equine arteritis virus; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; D037521:Virulence Factors
keywords: Apoptosis; Equine arteritis virus; Insect cells; gP5 protein