title: The effect of ileal interposition surgery on enteroendocrine cell numbers in the UC Davis type 2 diabetes mellitus rat.
abstract: OBJECTIVE
  To investigate the short-term effect of ileal interposition (IT) surgery on gut morphology and enteroendocrine cell numbers in the pre-diabetic UC Davis type 2 diabetes mellitus (UCD-T2DM) rat.
  
  
  METHODS
  Two-month old male UCD-T2DM rats underwent either sham (n=5) or IT (n=5) surgery. Intestines were collected 1.5months after surgery. The jejunum, ileum and colon regions were processed for histochemical and immunohistochemical labeling and stereological analyses of changes in gut morphometry and number of enteroendocrine cells.
  
  
  RESULTS
  Stereological analysis showed that intestinal volume, luminal surface area and the number of all chromogranin A-positive enteroendocrine cells were markedly increased in the IT rats compared with sham-operated animals. Subanalyses of the glucagon-like peptide 2, cholecystokinin, serotonin cells and the neurotensin immunoreactive sub-pool of enteroendocrine cells in the IT region revealed an increase in numbers across phenotypes. However, the density of the different cell types varied.
  
  
  CONCLUSIONS
  IT surgery in the UCD-T2DM rat leads to rapid alterations in gut morphometry and an increase in the number of enteroendocrine cells. This effect may potentially explain why IT surgery delays the onset of type 2 diabetes in the UCD-T2DM rat.
authors: Gubra, Hørsholm, Denmark; Department of Human Nutrition, University of Copenhagen, Frederiksberg, Denmark.
authors: Carl Frederik
authors: CF
authors: Hansen
authors: Gubra, Hørsholm, Denmark.
authors: Efstathios
authors: E
authors: Vassiliadis
authors: Gubra, Hørsholm, Denmark.
authors: Niels
authors: N
authors: Vrang
authors: Department of Human Nutrition, University of Copenhagen, Frederiksberg, Denmark.
authors: Per T
authors: PT
authors: Sangild
authors: Department of Biomedical Sciences, School of Veterinary Medicine, Cornell University, Ithaca, NY, USA.
authors: Bethany P
authors: BP
authors: Cummings
authors: Department of Molecular Biosciences, University of California, Davis, CA, USA.
authors: Peter
authors: P
authors: Havel
authors: Gubra, Hørsholm, Denmark. Electronic address: jacob@gubra.dk.
authors: Jacob
authors: J
authors: Jelsing
mesh_terms: D000818:Animals; D002766:Cholecystokinin / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D019858:Enteroendocrine Cells / Q000166:cytology*; D053767:Glucagon-Like Peptide 2 / Q000378:metabolism; D007082:Ileum / Q000378:metabolism*; D050356:Lipid Metabolism; D008297:Male; D051381:Rats; D012701:Serotonin / Q000378:metabolism
chemical_list: D053767:Glucagon-Like Peptide 2; D012701:Serotonin; D002766:Cholecystokinin
keywords: Bariatric surgery; Enteroendocrine cells; Ileal interposition; Stereology