title: Prognostic significance of miRNA-1 (miR-1) expression in patients with chordoma.
abstract: Reliable prognostic biomarkers for chordoma have not yet been established. Recent studies revealed that expression of miRNA-1 (miR-1) is frequently downregulated in several cancer types including chordoma. The goal of this follow-up study is to investigate the expression of miR-1 as a prognostic biomarker and further confirm the functional role of miR-1 in chordoma cell growth and proliferation. We determined the relative expression levels of miR-1 and Met in chordoma tissue samples and correlated those to clinical variables. The results showed that miR-1 was downregulated in 93.7% of chordoma tissues and expression was inversely correlated with Met expression. miR-1 expression levels also correlated with clinical prognosis. To characterize and confirm the functional role of miR-1 in the growth and proliferation of chordoma cells, miR-1 precursors were stably transfected into chordoma cell lines UCH-1 and CH-22. Cell Proliferation Assay and MTT were used to evaluate cell growth and proliferation. Restoring expression of miR-1 precursor decreased cell growth and proliferation in UCH-1 and CH-22 cells. These results indicate that suppressed miR-1 expression in chordoma may in part be a driver for tumor growth, and that miR-1 has potential to serve as prognostic biomarker and therapeutic target for chordoma patients.
authors: Sarcoma Biology Laboratory, Department of Orthopedic Surgery, Massachusetts General Hospital, Boston, Massachusetts, 02114.
authors: Zhenfeng
authors: Z
authors: Duan
authors: Jacson
authors: J
authors: Shen
authors: Xiaoqian
authors: X
authors: Yang
authors: Pei
authors: P
authors: Yang
authors: Eiji
authors: E
authors: Osaka
authors: Edwin
authors: E
authors: Choy
authors: Gregory
authors: G
authors: Cote
authors: David
authors: D
authors: Harmon
authors: Yu
authors: Y
authors: Zhang
authors: G Petur
authors: GP
authors: Nielsen
authors: Dimitrios
authors: D
authors: Spentzos
authors: Henry
authors: H
authors: Mankin
authors: Francis
authors: F
authors: Hornicek
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D045744:Cell Line, Tumor; D002817:Chordoma / Q000235:genetics; D015536:Down-Regulation; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis*; D008875:Middle Aged; D011379:Prognosis; D019859:Proto-Oncogene Proteins c-met / Q000096:biosynthesis
chemical_list: D014408:Biomarkers, Tumor; C544547:MIRN1 microRNA, human; D035683:MicroRNAs; C491743:MET protein, human; D019859:Proto-Oncogene Proteins c-met
keywords: Met; chordoma; miR-1; prognostic biomarker