title: Adiponectin effect on the viability of human endometrial stromal cells and mRNA expression of adiponectin receptors.
abstract: BACKGROUND
  Adiponectin is one of the most important adipokines secreted from fatty tissue that has a direct inhibitory effect on the development of cancer cells. Adiponectin plays an important role in human reproduction system and fertility of women. Adiponectin concentration decreases in women with endometriosis and endometrial cancer. The aim of the present study was to investigate the effect of adiponectin on human endometrial stromal cell (HESC) viability as well as mRNA expression of Adipo R1 and Adipo R2 receptors.
  
  
  METHODS
  In this experimental study, eight endometrial biopsies were taken and stromal cells were separated by enzymatic digestion and cell filtrations. Stromal cells of each biopsy were divided into four groups: control, 10, 100, and 200 ng/ml adiponectin concentrations. The effect of adiponectin on viability of the normal HESCs was studied by trypan blue staining and the relative expression levels of Adipo R1 and R2 were analyzed by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). Data were analyzed by one way ANOVA and unpaired student's t test and p<0.05 was considered significant.
  
  
  RESULTS
  Adiponectin decreased viability of normal human endometrial stromal cells in a dose and time dependent manner. Expression of Adipo R1 and Adipo R2 receptors did not change in the presence of adiponectin.
  
  
  CONCLUSIONS
  Adiponectin can directly influence the viability of HESCs and decrease their viability, but it didn't change expression of adiponectin receptors.
authors: Department of Veterinary Physiology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
authors: Somayeh
authors: S
authors: Bohlouli
authors: Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors: Mozafar
authors: M
authors: Khazaei
authors: Department of Veterinary Physiology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
authors: Masoud
authors: M
authors: Teshfam
authors: Department of Basic Sciences (Veterinary Physiology Division), Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
authors: Hosein
authors: H
authors: Hassanpour
keywords: Adipo R1; Adipo R2; Adiponectin; Endometrium; Stromal Cells