title: Role of activating transcription factor 3 protein ATF3 in necrosis and apoptosis induced by 5-fluoro-2'-deoxyuridine.
abstract: Necrosis and apoptosis are the two major forms of cell death. We have studied the mechanisms that regulate the cell death observed during treatment of mouse cancer cell line FM3A with the anticancer drug 5-fluoro-2'-deoxyuridine (FUdR). To detect causal differences between necrosis and apoptosis, we exploited the necrosis in original clone F28-7 and the apoptosis in its variant F28-7-A that occur on treatment with FUdR. Activating transcription factor 3 (ATF3) was strongly induced during necrosis but not apoptosis. In addition, we found that ATF3 expression is regulated by heat shock protein 90 (HSP90) at the mRNA stage. Knockdown of Atf3 by siRNA in the F28-7 cells resulted in apoptotic morphology rather than necrotic morphology. These results suggest that ATF3 is a cell-death regulator in necrosis and apoptosis.
authors: Faculty of Pharmaceutical Sciences, Okayama University, Kita-ku, Japan; Division of Genome Stability Research, National Cancer Center Research Institute, Chuo-ku, Tokyo, Japan.
authors: Akira
authors: A
authors: Sato
authors: Kentaro
authors: K
authors: Nakama
authors: Hiroki
authors: H
authors: Watanabe
authors: Akito
authors: A
authors: Satake
authors: Akihiro
authors: A
authors: Yamamoto
authors: Takuya
authors: T
authors: Omi
authors: Akiko
authors: A
authors: Hiramoto
authors: Mitsuko
authors: M
authors: Masutani
authors: Yusuke
authors: Y
authors: Wataya
authors: Hye-Sook
authors: HS
authors: Kim
mesh_terms: D051700:Activating Transcription Factor 3 / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D005467:Floxuridine / Q000633:toxicity; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D009336:Necrosis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D051700:Activating Transcription Factor 3; C103002:Atf3 protein, mouse; D018841:HSP90 Heat-Shock Proteins; D012333:RNA, Messenger; D005467:Floxuridine
keywords: activating transcription factor 3 (ATF3); apoptosis; cell death; heat shock protein 90 (HSP90); necrosis