title: Optimization of milk-based medium for efficient cultivation of Bifidobacterium pseudocatenulatum G4 using face-centered central composite-response surface methodology.
abstract: This study was undertaken to optimize skim milk and yeast extract concentration as a cultivation medium for optimal Bifidobacteria pseudocatenulatum G4 (G4) biomass and β -galactosidase production as well as lactose and free amino nitrogen (FAN) balance after cultivation period. Optimization process in this study involved four steps: screening for significant factors using 2(3) full factorial design, steepest ascent, optimization using FCCD-RSM, and verification. From screening steps, skim milk and yeast extract showed significant influence on the biomass production and, based on the steepest ascent step, middle points of skim milk (6% wt/vol) and yeast extract (1.89% wt/vol) were obtained. A polynomial regression model in FCCD-RSM revealed that both factors were found significant and the strongest influence was given by skim milk concentration. Optimum concentrations of skim milk and yeast extract for maximum biomass G4 and β -galactosidase production meanwhile low in lactose and FAN balance after cultivation period were 5.89% (wt/vol) and 2.31% (wt/vol), respectively. The validation experiments showed that the predicted and experimental values are not significantly different, indicating that the FCCD-RSM model developed is sufficient to describe the cultivation process of G4 using skim-milk-based medium with the addition of yeast extract.
authors: Department of Biomolecular Sciences, Faculty of Applied Sciences, Universiti Teknologi MARA, Sec. 2, 40150 Shah Alam, Selangor, Malaysia.
authors: Khalilah
authors: K
authors: Abdul Khalil
authors: Halal Products Research Institute, Universiti Putra Malaysia, Putra Infoport, 43400 Serdang, Selangor, 40150 Shah Alam, Selangor, Malaysia ; Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 13 Serdang, Selangor, Malaysia.
authors: Shuhaimi
authors: S
authors: Mustafa
authors: Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 13 Serdang, Selangor, Malaysia ; Institute of Tropical Forestry and Forest Products, Universiti Putra Malaysia, 43400 15 Serdang, Selangor, Malaysia.
authors: Rosfarizan
authors: R
authors: Mohammad
authors: Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 13 Serdang, Selangor, Malaysia.
authors: Arbakariya
authors: A
authors: Bin Ariff
authors: Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 13 Serdang, Selangor, Malaysia.
authors: Yamin
authors: Y
authors: Shaari
authors: Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors: Yazid
authors: Y
authors: Abdul Manap
authors: Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 13 Serdang, Selangor, Malaysia.
authors: Siti Aqlima
authors: 0000-0002-7625-3704
authors: SA
authors: Ahmad
authors: The School of Environmental Engineering, Universiti Malaysia Perlis, Kompleks Pengajian Jejawi 3, 02600 Arau, Perlis, Malaysia.
authors: Farrah Aini
authors: FA
authors: Dahalan
mesh_terms: D000818:Animals; D001644:Bifidobacterium / Q000254:growth & development* / Q000378:metabolism*; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism; D008892:Milk; D015233:Models, Statistical; D015203:Reproducibility of Results; D012107:Research Design; D015003:Yeasts
chemical_list: D003470:Culture Media