title: [Impact of an automated rapid MRSA/VRE identification technique in the surveillance of patients transferred from other medical centers].
abstract: BACKGROUND
  Identification of patients with methicillin resistant Staphylococcus aureus (MRSA) and vancomycin resistant Enterococci (VRE) is essential to limit the spread of these agents, through the use of isolation and contact precautions. Traditional microbiology has a long turn around time (3-5 days) extending the time of isolation, increasing complexity and cost of these patients.
  
  
  OBJECTIVE
  To implement a new real time polymerase chain reaction (PCR) GeneXpert R for SAMR and VRE detection. To compare costs and turn around time of PCR versus traditional cultures.
  
  
  METHODS
  Two periods were compared, in the first, traditional microbiology (standard group) was used, and in the second, only PCR was used (PCR group).
  
  
  RESULTS
  MRSA or VRE were identified in 29.9% of patients in the PCR group and in 9.6% in the standard group. Turn around time was 15 ± 9 hours in PCR group and 53 ± 23 hours in standard group. PCR group had a net cost of USD 245 per patient and standard group USD 530 per patient.
  
  
  CONCLUSIONS
  PCR technique GeneXpert R for MRSA and VRE had a positive impact in the management of these patients and justifies its inclusion.
authors: Alvaro
authors: A
authors: Rojas
authors: Marcela
authors: M
authors: Potin
authors: Claudia
authors: C
authors: Corla
authors: Juan C
authors: JC
authors: Román
authors: Patricia
authors: P
authors: García
mesh_terms: D016983:Enterococcus / Q000187:drug effects / Q000302:isolation & purification*; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000302:isolation & purification*; D010360:Patient Transfer; D060888:Real-Time Polymerase Chain Reaction / Q000191:economics* / Q000379:methods; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000191:economics / Q000382:microbiology*; D013997:Time Factors; D020713:Vancomycin Resistance