title: Upregulation of TLR2 expression is induced by estrogen via an estrogen-response element (ERE).
abstract: TLR2 and estrogen are both thought to be involved in the pathogenesis of RA; however, it is unknown if there is an association between estrogen and TLR2. In this report, we treated PMA-differentiated THP-1 cells with 17β-estradiol (E2) and observed increases in TLR2 mRNA and protein levels by real-time quantitative PCR and western blot. Transfection of THP-1 cells with a series of 5'-deleted TLR2 promoter-luciferase constructs revealed that E2 enhanced TLR2 transcriptional activity in an estrogen receptor alpha (ERα)-dependent pattern. An estrogen receptor response element (ERE) was identified 251 bases upstream of the TLR2 promoter, and electrophoretic mobility shift assay and chromatin immunoprecipitations showed ERα binding was increased by E2. In summary, this work demonstrated that TLR2 is a new estrogen-regulated gene whose expression is upregulated through the interaction of ERα with an ERE in the promoter region.
authors: Department of Orthopedics Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang 110032, China; Department of Sports Medicine and Joint Surgery, The First Affiliated Hospital, China Medical University, Shenyang 110001, China.
authors: Xi
authors: X
authors: Li
authors: Department of Pathogen Biology, College of Basic Medical Sciences, China Medical University, Shenyang 110001, China.
authors: Miao
authors: M
authors: Li
authors: Department of Sports Medicine and Joint Surgery, The First Affiliated Hospital, China Medical University, Shenyang 110001, China. Electronic address: baixizhuangcmu@sina.com.
authors: Xizhuang
authors: X
authors: Bai
mesh_terms: D002460:Cell Line; D004958:Estradiol / Q000494:pharmacology*; D047628:Estrogen Receptor alpha / Q000378:metabolism*; D004967:Estrogens / Q000494:pharmacology*; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020218:Response Elements / Q000187:drug effects* / Q000235:genetics; D051195:Toll-Like Receptor 2 / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D047628:Estrogen Receptor alpha; D004967:Estrogens; D012333:RNA, Messenger; C495342:TLR2 protein, human; D051195:Toll-Like Receptor 2; D004958:Estradiol
keywords: 17β-Estradiol; ERE; Gene expression; TLR2