title: Intraspinal transplantation of mouse and human neural precursor cells.
abstract: This unit describes the preparation and transplantation of human neural precursor cells (hNPCs) and mouse neural precursor cells (mNPCs) into the thoracic region of the mouse spinal cord. The techniques in this unit also describe how to prepare the mouse for surgery by performing a laminectomy to expose the spinal cord for transplantation. NPCs genetically labeled with eGFP transplanted into the spinal cord of a mouse following viral-mediated demyelination can efficiently be detected via eGFP expression. Transplantation of these cells into the spinal cord is an efficacious way to determine their effects in neurological disorders such as multiple sclerosis, Alzheimer's disease, and spinal cord injury.
authors: Department of Molecular Biology and Biochemistry, Sue and Bill Gross Stem Cell Center, Multiple Sclerosis Research Center, University of California, Irvine, California.
authors: Jason G
authors: JG
authors: Weinger
authors: Department of Molecular Biology and Biochemistry, Sue and Bill Gross Stem Cell Center, Multiple Sclerosis Research Center, University of California, Irvine, California.
authors: Lu
authors: L
authors: Chen
authors: Center for Regenerative Medicine, Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California.
authors: Ronald
authors: R
authors: Coleman
authors: Department of Molecular Biology and Biochemistry, Sue and Bill Gross Stem Cell Center, Multiple Sclerosis Research Center, University of California, Irvine, California.
authors: Ronika
authors: R
authors: Leang
authors: Department of Molecular Biology and Biochemistry, Sue and Bill Gross Stem Cell Center, Multiple Sclerosis Research Center, University of California, Irvine, California.
authors: Warren C
authors: WC
authors: Plaisted
authors: Center for Regenerative Medicine, Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California.
authors: Jeanne F
authors: JF
authors: Loring
authors: Department of Molecular Biology and Biochemistry, Sue and Bill Gross Stem Cell Center, Multiple Sclerosis Research Center, University of California, Irvine, California.
authors: Thomas E
authors: TE
authors: Lane
mesh_terms: D000818:Animals; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology* / Q000637:transplantation*; D013116:Spinal Cord / Q000473:pathology* / Q000601:surgery; D033581:Stem Cell Transplantation / Q000379:methods*
chemical_list: D049452:Green Fluorescent Proteins
keywords: human neural precursor cells; intraspinal transplantation; laminectomy; mouse neural precursor cells; multipotency