title: A Drosophila in vivo screen identifies store-operated calcium entry as a key regulator of adiposity.
abstract: To unravel the evolutionarily conserved genetic network underlying energy homeostasis, we performed a systematic in vivo gene knockdown screen in Drosophila. We used a transgenic RNAi library enriched for fly orthologs of human genes to functionally impair about half of all Drosophila genes specifically in adult fat storage tissue. This approach identified 77 genes, which affect the body fat content of the fly, including 58 previously unknown obesity-associated genes. These genes function in diverse biological processes such as lipid metabolism, vesicle-mediated trafficking, and the universal store-operated calcium entry (SOCE). Impairment of the SOCE core component Stromal interaction molecule (Stim), as well as other components of the pathway, causes adiposity in flies. Acute Stim dysfunction in the fat storage tissue triggers hyperphagia via remote control of the orexigenic short neuropeptide F in the brain, which in turn affects the coordinated lipogenic and lipolytic gene regulation, resulting in adipose tissue hypertrophy.
authors: Abteilung Molekulare Entwicklungsbiologie, Max-Planck-Institut für biophysikalische Chemie, 37077 Göttingen, Germany; Forschungsgruppe Molekulare Physiologie, Max-Planck-Institut für biophysikalische Chemie, 37077 Göttingen, Germany.
authors: Jens
authors: J
authors: Baumbach
authors: IT und Elektronik Service, Max-Planck-Institut für biophysikalische Chemie, 37077 Göttingen, Germany.
authors: Petra
authors: P
authors: Hummel
authors: Abteilung Molekulare Entwicklungsbiologie, Max-Planck-Institut für biophysikalische Chemie, 37077 Göttingen, Germany.
authors: Iris
authors: I
authors: Bickmeyer
authors: Abteilung Molekulare Entwicklungsbiologie, Max-Planck-Institut für biophysikalische Chemie, 37077 Göttingen, Germany.
authors: Katarzyna M
authors: KM
authors: Kowalczyk
authors: Abteilung Molekulare Entwicklungsbiologie, Max-Planck-Institut für biophysikalische Chemie, 37077 Göttingen, Germany.
authors: Martina
authors: M
authors: Frank
authors: Abteilung Molekulare Entwicklungsbiologie, Max-Planck-Institut für biophysikalische Chemie, 37077 Göttingen, Germany.
authors: Konstantin
authors: K
authors: Knorr
authors: Abteilung Molekulare Entwicklungsbiologie, Max-Planck-Institut für biophysikalische Chemie, 37077 Göttingen, Germany; Forschungsgruppe Molekulare Physiologie, Max-Planck-Institut für biophysikalische Chemie, 37077 Göttingen, Germany.
authors: Anja
authors: A
authors: Hildebrandt
authors: Facility für Elektronenmikroskopie, Max-Planck-Institut für biophysikalische Chemie, 37077 Göttingen, Germany.
authors: Dietmar
authors: D
authors: Riedel
authors: Abteilung Molekulare Entwicklungsbiologie, Max-Planck-Institut für biophysikalische Chemie, 37077 Göttingen, Germany.
authors: Herbert
authors: H
authors: Jäckle
authors: Abteilung Molekulare Entwicklungsbiologie, Max-Planck-Institut für biophysikalische Chemie, 37077 Göttingen, Germany; Forschungsgruppe Molekulare Physiologie, Max-Planck-Institut für biophysikalische Chemie, 37077 Göttingen, Germany. Electronic address: rkuehnl@gwdg.de.
authors: Ronald P
authors: RP
authors: Kühnlein
mesh_terms: D050154:Adiposity / Q000235:genetics / Q000502:physiology*; D000818:Animals; D001921:Brain / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D004330:Drosophila; D008954:Models, Biological; D009479:Neuropeptides / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D043743:Ubiquitin-Protein Ligase Complexes
chemical_list: D002118:Calcium; D009479:Neuropeptides; D011506:Proteins; C527990:Wdtc1 protein, mouse; D043743:Ubiquitin-Protein Ligase Complexes; C076101:neuropeptide F