title: Quercetin mitigates lead acetate-induced behavioral and histological alterations via suppression of oxidative stress, Hsp-70, Bak and upregulation of Bcl-2.
abstract: Lead toxicity is of major health concern due to its persistence in environment that induces cognitive impairment and neuronal degeneration. The present study was conducted to investigate the efficacy of quercetin, a ubiquitous bioflavonoid against lead-induced neurotoxicity in Wistar rats. Briefly, lead acetate (20mg/kg) was injected i.p., followed by oral administration of quercetin (50 and 100mg/kg) once daily for five consecutive days. On 6th day, rats were assessed for motor co-ordination, grip strength and sensorimotor impairment (by adhesive removal test). Lead treated rats have shown marked behavioral impairment with increased oxidative stress. Quercetin reduced lead-induced oxidative burden in brain, thus maintained the normal behavioral functions of lead-intoxicated rats. The lead administered group showed severely vacuolated and pyknotic nuclei with high expressions of Bak and Hsp-70. The expression of anti-apoptotic Bcl-2 was observed to be reduced in lead intoxicated group. Quercetin however, restored the normal morphology of brain and the expressions of Bak, Bcl-2 and Hsp-70. In conclusion, quercetin mitigates the toxic effect of lead effectively and thus, may be an important compound for developing effective therapeutic intervention against metal toxicity.
authors: Neurotoxicology Laboratory, Department of Medical Elementology & Toxicology (DST-FIST and UGC-SAP-BSR Funded Department), Jamia Hamdard (Hamdard University), Hamdard Nagar, New Delhi 110062, India.
authors: Krishan
authors: K
authors: Chander
authors: Neurotoxicology Laboratory, Department of Medical Elementology & Toxicology (DST-FIST and UGC-SAP-BSR Funded Department), Jamia Hamdard (Hamdard University), Hamdard Nagar, New Delhi 110062, India; Dr. B.R. Ambedkar Centre for Biomedical Research, University of Delhi, New Delhi, India.
authors: Kumar
authors: K
authors: Vaibhav
authors: Neurotoxicology Laboratory, Department of Medical Elementology & Toxicology (DST-FIST and UGC-SAP-BSR Funded Department), Jamia Hamdard (Hamdard University), Hamdard Nagar, New Delhi 110062, India.
authors: Md
authors: M
authors: Ejaz Ahmed
authors: Neurotoxicology Laboratory, Department of Medical Elementology & Toxicology (DST-FIST and UGC-SAP-BSR Funded Department), Jamia Hamdard (Hamdard University), Hamdard Nagar, New Delhi 110062, India.
authors: Hayate
authors: H
authors: Javed
authors: Neurotoxicology Laboratory, Department of Medical Elementology & Toxicology (DST-FIST and UGC-SAP-BSR Funded Department), Jamia Hamdard (Hamdard University), Hamdard Nagar, New Delhi 110062, India.
authors: Rizwana
authors: R
authors: Tabassum
authors: Neurotoxicology Laboratory, Department of Medical Elementology & Toxicology (DST-FIST and UGC-SAP-BSR Funded Department), Jamia Hamdard (Hamdard University), Hamdard Nagar, New Delhi 110062, India.
authors: Andleeb
authors: A
authors: Khan
authors: Dr. B.R. Ambedkar Centre for Biomedical Research, University of Delhi, New Delhi, India.
authors: Mukesh
authors: M
authors: Kumar
authors: Dr. B.R. Ambedkar Centre for Biomedical Research, University of Delhi, New Delhi, India.
authors: Anju
authors: A
authors: Katyal
authors: Neurotoxicology Laboratory, Department of Medical Elementology & Toxicology (DST-FIST and UGC-SAP-BSR Funded Department), Jamia Hamdard (Hamdard University), Hamdard Nagar, New Delhi 110062, India.
authors: Fakhrul
authors: F
authors: Islam
authors: Neurotoxicology Laboratory, Department of Medical Elementology & Toxicology (DST-FIST and UGC-SAP-BSR Funded Department), Jamia Hamdard (Hamdard University), Hamdard Nagar, New Delhi 110062, India. Electronic address: mohdssiddiqui@rediffmail.com.
authors: M Saeed
authors: MS
authors: Siddiqui
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000473:pathology; D003072:Cognition Disorders / Q000188:drug therapy / Q000209:etiology; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D007855:Lead Poisoning / Q000188:drug therapy / Q000209:etiology; D008297:Male; D009942:Organometallic Compounds / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D011794:Quercetin / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation; D050998:bcl-2 Homologous Antagonist-Killer Protein / Q000235:genetics / Q000378:metabolism*; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: C494774:Bak1 protein, rat; C494828:Bax protein, rat; D018840:HSP70 Heat-Shock Proteins; D009942:Organometallic Compounds; D050998:bcl-2 Homologous Antagonist-Killer Protein; D051028:bcl-2-Associated X Protein; D011794:Quercetin; C008261:lead acetate
keywords: Bak; Bcl-2; Hsp-70; Lead; Oxidative stress; Quercetin