title: Interferon-α suppresses invasion and enhances cisplatin-mediated apoptosis and autophagy in human osteosarcoma cells.
abstract: Interferon (IFN)-α is generated in response to viral infections and is used clinically in the therapy of a variety of viral infections and cancers. The present study investigated whether IFN-α could inhibit the invasive ability of osteosarcoma cells using a Matrigel invasion assay. In addition, the osteosarcoma cells were treated with cisplatin and/or IFN-α. Apoptosis and autophagy were assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, Hoechst 33258 staining, flow cytometry assay, acridine orange staining, green fluorescent protein-LC3 dot assay and transmission electron microscopy. Further analysis revealed that the efficacy of cisplatin was enhanced by the addition of the cytokine, IFN-α. These results indicate that the combination therapy of chemotherapeutics and IFN-α is a new approach for osteosarcoma, which requires validation by experimentsin vivo.
authors: Department of Parasitology, Xiangya Medical School, Central South University, Changsha, Hunan 410078, P.R. China ; Department of Microbiology, Anhui Medical University, Hefei, Anhui 230032, P.R. China.
authors: Jun
authors: J
authors: Zhao
authors: Department of Microbiology, Anhui Medical University, Hefei, Anhui 230032, P.R. China.
authors: Mingli
authors: M
authors: Wang
authors: Department of Microbiology, Anhui Medical University, Hefei, Anhui 230032, P.R. China.
authors: Zeng
authors: Z
authors: Li
authors: Department of Pathology and Cell Biology, Columbia University, New York, NY 10032, USA.
authors: Jingxian
authors: J
authors: Chen
authors: Department of Orthopedics, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230032, P.R. China.
authors: Zongshen
authors: Z
authors: Yin
authors: Department of Orthopedics, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230032, P.R. China.
authors: Jun
authors: J
authors: Chang
authors: Department of Clinical Laboratory, The Third Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230032, P.R. China.
authors: Dongmei
authors: D
authors: Gao
authors: Department of Parasitology, Xiangya Medical School, Central South University, Changsha, Hunan 410078, P.R. China.
authors: Shiping
authors: S
authors: Wang
keywords: apoptosis; autophagy; cisplatin; interferon-α; osteosarcoma cell