title: Preparation and evaluation of polysaccharide sulfates for inhibiting Helicobacter pylori adhesion.
abstract: In treatments of Helicobacter pylori infections, recrudescences were common because of an unfavorable bacterial eradication rate due to the ever increasing resistance to antibiotics. In this study, we chose pectin, guar gum and chitosan to synthesize their sulfates to inhibit adhesions of H. pylori and thus enhance the eradication rate. The introduction of sulfates was characterized using FT-IR and elemental analysis. Data from zeta-potential, hydrodynamic diameter, hydrolysis and rheological property demonstrated the sulfates were physicochemically stable. Inhibition assay of hemagglutination and adhesion indicated sulfates prevented H. pylori from adhering to erythrocytes and AGS cells. In binding assay, affinities of sulfates to H. pylori suggested sulfates could compete with target cells for bacteria and moderated the bacterial adhesion to hosts. A higher content of galactoses and 2,3-O-linked sulfates benefited this action. Thus polysaccharide sulfates can serve as potential adjuvants to raise the bacterial eradication rate by inhibiting adhesions of H. pylori.
authors: School of Pharmaceutical Sciences, Sun Yat-sen University, University Town, Guangzhou 510006, PR China; Department of Clinical Pharmacy, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou 450002, PR China.
authors: Weijuan
authors: W
authors: Song
authors: School of Pharmaceutical Sciences, Sun Yat-sen University, University Town, Guangzhou 510006, PR China.
authors: Yalong
authors: Y
authors: Wang
authors: Guangdong General Hospital, Guangzhou 510080, PR China.
authors: Liyan
authors: L
authors: Zhang
authors: School of Pharmaceutical Sciences, Sun Yat-sen University, University Town, Guangzhou 510006, PR China.
authors: Shengnan
authors: S
authors: Fu
authors: Guangdong General Hospital, Guangzhou 510080, PR China.
authors: Ying
authors: Y
authors: Zeng
authors: School of Pharmaceutical Sciences, Sun Yat-sen University, University Town, Guangzhou 510006, PR China. Electronic address: lsshhy@mail.sysu.edu.cn.
authors: Haiyan
authors: H
authors: Hu
mesh_terms: D018829:Adhesins, Bacterial / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D016480:Helicobacter pylori / Q000187:drug effects*; D011134:Polysaccharides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013431:Sulfates / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D018829:Adhesins, Bacterial; D011134:Polysaccharides; D013431:Sulfates
keywords: Adhesion inhibition; Binding affinity; Helicobacter pylori; Polysaccharide sulfate