title: Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD.
abstract: The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.
authors: Laboratório de Doenças de Animais Aquáticos, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors: C A G
authors: CA
authors: Leal
authors: Laboratório de Doenças de Animais Aquáticos, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors: G A
authors: GA
authors: Carvalho-Castro
authors: Laboratório Nacional Agropecuário, Ministério da Agricultura, Pedro Leopoldo, MG, Brazil.
authors: A C
authors: AC
authors: Cottorello
authors: Laboratório de Doenças de Animais Aquáticos, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors: R C
authors: RC
authors: Leite
authors: Laboratório de Doenças de Animais Aquáticos, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors: H C P
authors: HC
authors: Figueiredo
mesh_terms: D000818:Animals; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D033561:Penaeidae / Q000821:virology*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014730:Veterinary Medicine / Q000379:methods*
keywords: WSD; WSSV; clinical samples; real-time PCR; sensitivity