title: Lyophilization as a novel approach for preparation of water resistant HA fiber membranes by crosslinked with EDC.
abstract: The hyaluronic acid (HA) fibers scaffold with an extracellular matrix mimic structure has been prepared via lyophilization. The morphology of the HA fibers varying the concentration from 0.05 wt% to 0.15 wt% was characterized by scanning electron microscope (SEM). The diameter of HA fibers increased and the morphology changed from fiber to sheet-like structure as the concentration of HA solution increased. To enhance the water-resistance, the pure HA fiber membranes were chemical cross-linked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), and confirmed by fourier transform infrared (FT-IR) spectra. In vitro degradation behavior of cross-linked HA fiber membranes in both of water and PBS solutions was investigated, the physical properties were also studied by differential scanning calorimetry (DSC) and thermogravimetry (TG). The results showed that the bonding water capacity increased after crosslinking, and indicated that the crosslinked HA fibers could be used as scaffold in tissue engineering.
authors: State Key Laboratory of Chemical Resource Engineering, Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors: Jie
authors: J
authors: Chen
authors: State Key Laboratory of Chemical Resource Engineering, Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors: Cheng
authors: C
authors: Peng
authors: State Key Laboratory of Chemical Resource Engineering, Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors: Jun
authors: J
authors: Nie
authors: Chembiotech Laboratories, University of Birmingham Research Park, Vincent Drive, Birmingham B15 2SQ, UK.
authors: John F
authors: JF
authors: Kennedy
authors: State Key Laboratory of Chemical Resource Engineering, Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, Beijing University of Chemical Technology, Beijing 100029, PR China. Electronic address: magp@mail.buct.edu.cn.
authors: Guiping
authors: G
authors: Ma
mesh_terms: D002152:Calorimetry, Differential Scanning; D002234:Carbodiimides / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D005612:Freeze Drying; D006820:Hyaluronic Acid / Q000737:chemistry*; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D017550:Spectroscopy, Fourier Transform Infrared; D054457:Tissue Scaffolds; D014867:Water / Q000737:chemistry*
chemical_list: C557098:1-ethyl-3-(3-dimethylaminoisopropyl) carbodiimide; D002234:Carbodiimides; D003432:Cross-Linking Reagents; D008567:Membranes, Artificial; D014867:Water; D006820:Hyaluronic Acid
keywords: Cross linked; Hyaluronic acid; Lyophilization; Tissue scaffold