title: Target-induced structure switching of DNA for label-free and ultrasensitive electrochemiluminescent detection of proteins.
abstract: The association of the target thrombin with the corresponding aptamer leads to structure switching of the dsDNA probes and the formation of nicking sites for exonuclease III, which causes cyclic cleavage of the dsDNA probes and highly reduced intercalation of the electrochemiluminescent signal indicators for label-free and sensitive detection of thrombin at the femtomolar level.
authors: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China. yunatswu@swu.edu.cn.
authors: Mengli
authors: M
authors: Yang
authors: Ying
authors: Y
authors: Chen
authors: Yun
authors: Y
authors: Xiang
authors: Ruo
authors: R
authors: Yuan
authors: Yaqin
authors: Y
authors: Chai
mesh_terms: D004277:DNA, Single-Stranded / Q000737:chemistry*; D055664:Electrochemical Techniques; D008163:Luminescent Measurements; D009690:Nucleic Acid Conformation; D013917:Thrombin / Q000032:analysis* / Q000378:metabolism
chemical_list: D004277:DNA, Single-Stranded; D013917:Thrombin