title: Immobilization of pectin degrading enzyme from Bacillus licheniformis KIBGE IB-21 using agar-agar as a support.
abstract: Pectinase from Bacillus licheniformis KIBGE IB-21 was immobilized in agar-agar matrix using entrapment technique. Effect of different concentrations of agar-agar on pectinase immobilization was investigated and it was found that maximum immobilization was achieved at 3.0% agar-agar with 80% enzyme activity. After immobilization, the optimum temperature of enzyme increased from 45 to 50 °C and reaction time from 5 to 10 minutes as compared to free enzyme. Due to the limited diffusion of high molecular weight substrate, K(m) of immobilized enzyme slightly increased from 1.017 to 1.055 mg ml(-1), while Vmax decreased from 23,800 to 19,392 μM min(-1) as compared to free enzyme. After 120 h entrapped pectinase retained their activity up to 82% and 71% at 30 °C and 40 °C, respectively. The entrapped pectinase showed activity until 10th cycle and maintain 69.21% activity even after third cycle.
authors: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi 75270, Pakistan.
authors: Haneef Ur
authors: HU
authors: Rehman
authors: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi 75270, Pakistan.
authors: Afsheen
authors: A
authors: Aman
authors: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi 75270, Pakistan.
authors: Raheela Rahmat
authors: RR
authors: Zohra
authors: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi 75270, Pakistan. Electronic address: ali_kibge@yahoo.com.
authors: Shah Ali Ul
authors: SA
authors: Qader
mesh_terms: D000362:Agar; D001407:Bacillus / Q000201:enzymology*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006358:Hot Temperature; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D011096:Polygalacturonase / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D004800:Enzymes, Immobilized; D000362:Agar; D011096:Polygalacturonase
keywords: Agar-agar; Bacillus licheniformis; Immobilization; Pectinase