title: High expression of sarcoplasmic/endoplasmic reticulum Ca(2+)-ATPase 2b blocks cell differentiation in human liposarcoma cells.
abstract: OBJECTIVE
  We have previously reported that elevated expression of sarcoplasmic/endoplasmic reticulum Ca(2+)-ATPase 2 (SERCA2) was related to the malignant degree of different types of human liposarcoma. Here, we investigated the effects of high SERCA2b expression on proliferation and differentiation of preadipocyte-like human liposarcoma cell line SW872 cells.
  
  
  METHODS
  SW872 cells were stably transfected with human SERCA2b expressing plasmid. Adipocyte differentiation was assayed by adipogenic gene and protein expression. Cell proliferation, formation of reactive oxygen species (ROS) and phosphorylation of peroxisome proliferator activated receptor gamma (PPAR-γ) and extracellular signal-regulated kinase (ERK) were determined by MTT assay, 2, 7-dichlorofluorescein diacetate (DCF-DA) assay and western blot analysis, respectively.
  
  
  RESULTS
  High expression of SERCA2b promoted cell proliferation and blocked the differentiation potential of SW872 cells under both in vitro and in vivo differentiation-inducing environment. Moreover, high expression of SERCA2b induced accumulation of ROS and enhanced ERK signaling, thus leading to inactivation of PPAR-γ and down-regulation of adipocyte-specific genes.
  
  
  CONCLUSIONS
  The results revealed a novel role of SERCA2b in facilitating the blockade of human liposarcoma differentiation, which helps provide a molecular target for therapeutic interventions of human liposarcoma.
authors: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China.
authors: Lu
authors: L
authors: Wang
authors: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China.
authors: Wanshuai
authors: W
authors: Li
authors: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China.
authors: Yang
authors: Y
authors: Yang
authors: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China.
authors: Yamei
authors: Y
authors: Hu
authors: Department of Clinical Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors: Yanhong
authors: Y
authors: Gu
authors: Department of Clinical Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors: Yongqian
authors: Y
authors: Shu
authors: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China.
authors: Yang
authors: Y
authors: Sun
authors: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China.
authors: Xuefeng
authors: X
authors: Wu
authors: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China. Electronic address: shenyan@nju.edu.cn.
authors: Yan
authors: Y
authors: Shen
authors: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China. Electronic address: molpharm@163.com.
authors: Qiang
authors: Q
authors: Xu
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008080:Liposarcoma / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D016513:Mice, SCID; D047495:PPAR gamma / Q000378:metabolism; D010766:Phosphorylation; D017382:Reactive Oxygen Species; D060888:Real-Time Polymerase Chain Reaction; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000235:genetics* / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D047495:PPAR gamma; D017382:Reactive Oxygen Species; D048049:Extracellular Signal-Regulated MAP Kinases; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases
keywords: Differentiation blockade; ERK; Human liposarcoma; PPAR-γ; SERCA2b; SW872 cells