title: First evidence of fish genotoxicity induced by heavy metals from landfill leachates: the advantage of using the RAPD-PCR technique.
abstract: Municipal leachates are loaded with heavy metals that can contaminate surface water before discharge into a receiving body of water. The aim of this study is to evaluate the genotoxic effects of heavy metals generated by domestic waste on the common roach Rutilus rutilus in the last of the four interconnected ponds at the Etueffont landfill. We used random amplified polymorphic DNA (RAPD) since it has been shown to be a powerful means of detecting a broad range of DNA damage due to environmental contaminants. Our results show the ability of RAPD analysis to detect significant genetic alterations in roach DNA, after contamination with a set of metals contained in the landfill leachates in comparison to a roach from a non-polluted reference pond. Analysis of electrophoresis profiles indicates apparent changes such as the appearance of new bands or disappearance of bands as compared to the control. In fact, mixed smearing and laddering of DNA fragments in muscle samples support the genotoxic effects of metal deposits in the roach. This study is the first evidence found via the RAPD-PCR technique in the detection of pollutant impacts on fish exposed to landfill leachates.
authors: Université de Franche-Comté, Laboratoire de Chrono-Environnement, UMR CNRS 6249, 1, Place Leclerc, F-25030 Besançon cedex, France; Université de Sfax, Faculté des Sciences de Sfax, Unité de recherche UR/11ES72, Biodiversité et Ecosystèmes Aquatiques, Route Soukra, CP 3000 Sfax, Tunisia.
authors: Zohra
authors: Z
authors: Ben Salem
authors: Université de Franche-Comté, Laboratoire de Chrono-Environnement, UMR CNRS 6249, 1, Place Leclerc, F-25030 Besançon cedex, France.
authors: Nicolas
authors: N
authors: Capelli
authors: Université de Franche-Comté, Laboratoire de Chrono-Environnement, UMR CNRS 6249, 1, Place Leclerc, F-25030 Besançon cedex, France.
authors: Elise
authors: E
authors: Grisey
authors: Université de Franche-Comté, Laboratoire de Chrono-Environnement, UMR CNRS 6249, 1, Place Leclerc, F-25030 Besançon cedex, France.
authors: Pierre-Emmanuel
authors: PE
authors: Baurand
authors: Université de Sfax, Faculté des Sciences de Sfax, Unité de recherche UR/11ES72, Biodiversité et Ecosystèmes Aquatiques, Route Soukra, CP 3000 Sfax, Tunisia.
authors: Habib
authors: H
authors: Ayadi
authors: Université de Franche-Comté, Laboratoire de Chrono-Environnement, UMR CNRS 6249, 1, Place Leclerc, F-25030 Besançon cedex, France. Electronic address: lotfi.aleya@univ-fcomte.fr.
authors: Lotfi
authors: L
authors: Aleya
mesh_terms: D000818:Animals; D003530:Cyprinidae / Q000502:physiology*; D004247:DNA / Q000187:drug effects*; D019216:Metals, Heavy / Q000032:analysis / Q000633:toxicity*; D018482:Muscle, Skeletal / Q000187:drug effects*; D019105:Random Amplified Polymorphic DNA Technique; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
chemical_list: D019216:Metals, Heavy; D014874:Water Pollutants, Chemical; D004247:DNA
keywords: Genotoxicity; Landfill; Leachates; Metals; Random amplified polymorphic DNA (RAPD) technique