title: Gene regulation by intracellular delivery and photodegradation of nanoparticles containing small interfering RNA.
abstract: Although the use of small interfering RNA (siRNA) is a promising technique for gene regulation, spatiotemporal control of the effects of siRNA must be achieved if the technique is to be safe and practical. Here, a method for spatiotemporal regulation of genes with nanoparticles containing siRNA is reported. The siRNA is encapsulated in photodegradable nanoparticles that are internalized to SKOV3-Luc cells, where the siRNA is released from the nanoparticles by UV irradiation for 30 s. The encapsulated siRNA only shows no gene-silencing effects, but release of the siRNA upon UV radiation leads to sequence-specific silencing of the luciferase gene in the cells. These results indicate that photodegradable siRNA-containing nanoparticles can be useful for time- and space-dependent regulation of gene expression in cells.
authors: Pharmaceutical Biotechnology, Center for System-based Drug Research, Center for NanoScience (CeNS), Ludwig-Maximilians-University, Butenandtstrasse 5-13, Munich, Germany; Graduate School of Pharmaceutical Sciences and GPLLI Program, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan; Molecular Imaging Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba, 263-8555, Japan.
authors: Shuhei
authors: S
authors: Murayama
authors: Petra
authors: P
authors: Kos
authors: Kanjiro
authors: K
authors: Miyata
authors: Kazunori
authors: K
authors: Kataoka
authors: Ernst
authors: E
authors: Wagner
authors: Masaru
authors: M
authors: Kato
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods*; D004587:Electrophoresis, Agar Gel; D024202:Electrophoretic Mobility Shift Assay; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D015394:Molecular Structure; D053758:Nanoparticles / Q000378:metabolism*; D010782:Photolysis; D034741:RNA, Small Interfering / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D034741:RNA, Small Interfering
keywords: gene delivery; nanoparticle; photodegradable; photoinduced; siRNA; spatiotemporal regulation