title: Mcp1p tracks microtubule plus ends to destabilize microtubules at cell tips.
abstract: Microtubule plus ends are dynamically regulated by a wide variety of proteins for performing diverse cellular functions. Here, we show that the fission yeast Schizosaccharomyces pombe uncharacterized protein mcp1p is a microtubule plus-end tracking protein which depends on the kinesin-8 klp6p for transporting along microtubules towards microtubule plus ends. In the absence of mcp1p, microtubule catastrophe and rescue frequencies decrease, leading to an increased dwell time of microtubule plus ends at cell tips. Thus, these findings suggest that mcp1p may synergize with klp6p at microtubule plus-ends to destabilize microtubules.
authors: HKU-Shenzhen Institute of Research and Innovation, The University of Hong Kong, Shenzhen, China; Department of Biochemistry, The University of Hong Kong, Pokfulam, Hong Kong, China.
authors: Fan
authors: F
authors: Zheng
authors: HKU-Shenzhen Institute of Research and Innovation, The University of Hong Kong, Shenzhen, China; Department of Biochemistry, The University of Hong Kong, Pokfulam, Hong Kong, China.
authors: Tianpeng
authors: T
authors: Li
authors: Department of Anatomy, The University of Hong Kong, Pokfulam, Hong Kong, China.
authors: Martin
authors: M
authors: Cheung
authors: Department of Cell and Developmental Biology, The University of Pennsylvania, Philadelphia, PA 19104, USA.
authors: Viktoriya
authors: V
authors: Syrovatkina
authors: HKU-Shenzhen Institute of Research and Innovation, The University of Hong Kong, Shenzhen, China; Department of Biochemistry, The University of Hong Kong, Pokfulam, Hong Kong, China. Electronic address: chuanhai@hku.hk.
authors: Chuanhai
authors: C
authors: Fu
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D016547:Kinesins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000502:physiology*; D008870:Microtubules / Q000378:metabolism*; D055550:Protein Stability; D021381:Protein Transport; D012568:Schizosaccharomyces / Q000378:metabolism* / Q000648:ultrastructure; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism / Q000502:physiology*; D059008:Time-Lapse Imaging
chemical_list: C588242:Mcp1 protein, S pombe; D008869:Microtubule-Associated Proteins; D029702:Schizosaccharomyces pombe Proteins; C459102:Klp6 protein, S pombe; D016547:Kinesins
keywords: +TIP; Kinesin; MAP; Microtubule; Microtubule depolymerization