title: Analysis of neutrophil bactericidal activity.
abstract: This chapter describes two methods for measuring the bactericidal activity of neutrophils. These are a new simple fluorescence-based assay, which quantifies bactericidal activity by measuring changes in bacterial fluorescence associated with a loss of membrane potential over time, and a more traditional colony counting protocol. Two variations of these techniques are presented: a "one-step" protocol providing a composite measure of phagocytosis and killing, and a "two-step" protocol that allows calculation of separate rate constants for both of these processes.
authors: Free Radical Research Group, Department of Pathology, Christchurch School of Medicine, Christchurch, New Zealand.
authors: Heather A
authors: HA
authors: Parker
authors: Nicholas J
authors: NJ
authors: Magon
authors: Jessie N
authors: JN
authors: Green
authors: Mark B
authors: MB
authors: Hampton
authors: Christine C
authors: CC
authors: Winterbourn
mesh_terms: D001419:Bacteria / Q000276:immunology*; D003114:Colony-Forming Units Assay; D005470:Fluorometry / Q000379:methods; D006801:Humans; D009504:Neutrophils / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology