title: Sphingomonas oligoaromativorans sp. nov., an oligotrophic bacterium isolated from a forest soil.
abstract: A halo- and organo-sensitive oligotrophic bacterium, designated strain SY-6T, was isolated from humus forest soil at Gyeryong mountain in Korea. Cells of the strain were Gram-negative, strictly aerobic, non-motile rods and the strain formed yellow-pigmented colonies on 100-fold-diluted nutrient broth. Strain SY-6T grew at pH 6.0-7.0 (optimal growth at pH 7.0), at 10-37 °C (optimal growth at 28 °C) and at salinities of 0-0.5% (w/v) NaCl, growing optimally at 0.01% (w/v) NaCl. On the basis of 16S rRNA gene sequence analysis, strain SY-6T was shown to belong to the genus Sphingomonas and showed the closest phylogenetic similarity to Sphingomonas polyaromaticivorans B2-7T (96.7%). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and sphingoglycolipid. The predominant ubiquinone and polyamine were Q-10 and sym-homospermidine, respectively. The major fatty acids were C18:1ω7c and C16:0. The DNA G+C content of the novel isolate was 65.3 mol%. On the basis of the evidence from this polyphasic study, strain SY-6T represents a novel species of the genus Sphingomonas, for which the name Sphingomonas oligoaromativorans sp. nov. is proposed. The type strain is SY-6T (=KACC 12948T=NBRC 105508T).
authors: Department of Biotechnology, Mokwon University, Doan-dong 800, Seo-gu, Daejeon 302-729, Republic of Korea.
authors: Song-Ih
authors: SI
authors: Han
authors: Institute of Microbial Ecology and Resources, Mokwon University, Doan-dong 800, Seo-gu, Daejeon 302-729, Republic of Korea.
authors: Jae-Chan
authors: JC
authors: Lee
authors: Department of Bioresource Science, College of Agriculture, Ibaraki University; Ami-machi, Ibaraki 300-0393, Japan.
authors: Hiroyuki
authors: H
authors: Ohta
authors: Institute of Microbial Ecology and Resources, Mokwon University, Doan-dong 800, Seo-gu, Daejeon 302-729, Republic of Korea.|Department of Biotechnology, Mokwon University, Doan-dong 800, Seo-gu, Daejeon 302-729, Republic of Korea.
authors: Kyung-Sook
authors: KS
authors: Whang
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013095:Spermidine / Q000031:analogs & derivatives / Q000737:chemistry; D020578:Sphingomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014197:Trees / Q000382:microbiology; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; C007961:sym-homospermidine; D014451:Ubiquinone; C025204:Ubiquinone Q2; D013095:Spermidine