title: Lens extrusion from Laminin alpha 1 mutant zebrafish.
abstract: We report analysis of the ocular lens phenotype of the recessive, larval lethal zebrafish mutant, lama1 (a69/a69). Previous work revealed that this mutant has a shortened body axis and eye defects including a defective hyaloid vasculature, focal corneal dysplasia, and loss of the crystalline lens. While these studies highlight the importance of laminin α1 in lens development, a detailed analysis of the lens defects seen in these mutants was not reported. In the present study, we analyze the lenticular anomalies seen in the lama1 (a69/a69) mutants and show that the lens defects result from the anterior extrusion of lens material from the eye secondary to structural defects in the lens capsule and developing corneal epithelium associated with basement membrane loss. Our analysis provides further insights into the role of the lens capsule and corneal basement membrane in the structural integrity of the developing eye.
authors: Department of Biological Sciences, University of Delaware, 327 Wolf Hall Newark, DE 19716, USA.
authors: Mallika
authors: 0000-0002-2729-9351
authors: M
authors: Pathania
authors: Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
authors: Elena V
authors: EV
authors: Semina
authors: Department of Biological Sciences, University of Delaware, 327 Wolf Hall Newark, DE 19716, USA.
authors: Melinda K
authors: MK
authors: Duncan
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000378:metabolism; D024141:Collagen Type IV / Q000378:metabolism; D003315:Cornea / Q000378:metabolism / Q000473:pathology; D007797:Laminin / Q000235:genetics* / Q000378:metabolism; D007903:Lens Capsule, Crystalline / Q000378:metabolism; D007908:Lens, Crystalline / Q000378:metabolism* / Q000473:pathology*; D009154:Mutation; D010641:Phenotype; D015027:Zebrafish / Q000235:genetics*; D029961:Zebrafish Proteins / Q000235:genetics*
chemical_list: D024141:Collagen Type IV; D007797:Laminin; D029961:Zebrafish Proteins; C510925:laminin alpha1, zebrafish