title: Identification of a frustule-associated protein of the marine pennate diatom Fistulifera sp. strain JPCC DA0580.
abstract: Among the proteins localized on the cell wall (frustule) of diatoms (frustule-associated proteins), several proteins tightly associated with the cell wall have been implicated in frustule formation. These proteins include diatom-specific unique serine- and lysine-rich sequences represented by silaffins. Taking advantage of available genome information, we used a recently described bioinformatics approach to screen silaffin-like proteins rich in serine and lysine from the genome of the marine pennate diatom Fistulifera sp. strain JPCC DA0580 and identified 7 proteins. All of the proteins shared a sequence motif called the XGXG domain, which was also confirmed in a silaffin-like protein identified in other diatoms. In vivo localization analysis revealed that one of the identified proteins, G7408, occurs throughout the frustule with a slightly uneven distribution. This novel frustule-associated protein could be a useful tool to elucidate the mechanism of biosilica formation in diatoms and to functionalize this strain for future biotechnological applications.
authors: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan.
authors: Michiko
authors: M
authors: Nemoto
authors: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan.
authors: Yoshiaki
authors: Y
authors: Maeda
authors: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan; JST, CREST, Sanbancho 5, Chiyoda-ku, Tokyo 102-0075, Japan.
authors: Masaki
authors: M
authors: Muto
authors: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan; JST, CREST, Sanbancho 5, Chiyoda-ku, Tokyo 102-0075, Japan.
authors: Masayoshi
authors: M
authors: Tanaka
authors: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan.
authors: Tomoko
authors: T
authors: Yoshino
authors: Department of Biology, Tokyo Gakugei University, 4-1-1, Nukuikita-machi, Koganei, Tokyo 184-8501, Japan.
authors: Shigeki
authors: S
authors: Mayama
authors: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan; JST, CREST, Sanbancho 5, Chiyoda-ku, Tokyo 102-0075, Japan. Electronic address: tsuyo@cc.tuat.ac.jp.
authors: Tsuyoshi
authors: T
authors: Tanaka
mesh_terms: D002473:Cell Wall / Q000737:chemistry / Q000235:genetics; D017377:Diatoms / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D016678:Genome; D010802:Phylogeny; D020543:Proteome; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D020543:Proteome; D015800:Protozoan Proteins
keywords: Biomineral; Fistulifera sp. strain JPCC DA0580; Frustule-associated protein; Marine diatom; Whole genome