title: Highly fluorescent conjugated polyelectrolyte for protein sensing and cell-compatible chemosensing applications.
abstract: Using a highly fluorescent, water-soluble polymer derived from a triazine-bridged copolymer (DTMSPV), we explored the tunable fluorescence properties of the water-soluble DTMSPV by solvent polarity to function as a fluorescence sensory probe for protein sensing. The green-blue fluorescence from DTMSPV was significantly enhanced in the presence of bovine serum albumin through hydrophobic interactions. Meanwhile, complete quenching of the fluorescence from DTMSPV occurred in the presence of hemoglobin through iron complexation with the polyelectrolyte. In addition, the DTMSPVs were highly fluorescent and permeated into living mesenchymal stem cells (MSCs), enabling effective imaging of the MSCs. This permeation into stem cells is crucial to the detection of Al(3+) in living MSCs. The interaction between the triazine units in DTMSPV with the Al(3+) ions allows for the detection of Al(3+) in living cells. Thus, a strong fluorescence from living MSCs pretreated with DTMSPV was quenched as a function of the Al(3+) concentration, confirming that DTMSPV is a cell-permeable fluorescent polymer that can function as a versatile probe to detect Al(3+) in living cells.
authors: Department of Chemical and Biomolecular Engineering, Yonsei University , and §Cell Therapy Center, Severance Hospital, and ⊥Department of Laboratory Medicine, Yonsei University College of Medicine , 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Korea.
authors: Jungmok
authors: J
authors: You
authors: Teahoon
authors: T
authors: Park
authors: Jeonghun
authors: J
authors: Kim
authors: June Seok
authors: JS
authors: Heo
authors: Han-Soo
authors: HS
authors: Kim
authors: Hyun Ok
authors: HO
authors: Kim
authors: Eunkyoung
authors: E
authors: Kim
mesh_terms: D000535:Aluminum / Q000032:analysis*; D000818:Animals; D002417:Cattle; D004573:Electrolytes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000737:chemistry* / Q000166:cytology*; D011506:Proteins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013552:Swine
chemical_list: D004573:Electrolytes; D005456:Fluorescent Dyes; D011506:Proteins; D000535:Aluminum