title: An aryl hydrocarbon receptor ligand acts on dendritic cells and T cells to suppress the Th17 response in allergic rhinitis patients.
abstract: A predominant Th17 population is a marker of allergic rhinitis (AR). The aryl hydrocarbon receptor (AhR) exhibits strong immunomodulation potential via regulation of the differentiation of T lymphocytes and dendritic cells (DCs) after activation by its ligand, such as 2-(1'H-indole-3'-carbonyl)-thiazole-4-carboxylic acid methyl ester (ITE). The aim of this study was to analyze the effect of AhR on Th17 differentiation by investigating the action of ITE on DCs and CD4(+) T cells from patients with AR. In all, 26 AR patients and 12 healthy controls were included in this study. The expression of interleukin (IL)-1β, IL-6, IL-10, and IL-17 in the culture supernatant and the presence of Th17 cells in CD4(+) T cells and DC-CD4(+) T-cell co-culture system were measured before and after treatment with ITE. We show that ITE significantly induced cell secretion of IL-10 and inhibited IL-1β and IL-6 production in DCs, and promoted IL-10 production and suppressed IL-17 expression in CD4(+) T cells in vitro. It also suppressed the expansion of Th17 cells in vitro. Our work demonstrates that ITE acts on DCs and CD4(+) T cells to inhibit the Th17 response that suppresses AR; the AhR-DC-Th17 axis may be an important pathway in the treatment of AR. ITE, a nontoxic AhR ligand, attenuated the Th17 response; thus, it appears to be a promising therapeutic candidate for suppressing the inflammatory responses associated with AR.
authors: Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors: Ping
authors: P
authors: Wei
authors: Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors: Guo-Hua
authors: GH
authors: Hu
authors: Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors: Hou-Yong
authors: HY
authors: Kang
authors: Department of Otolaryngology, The Children's Hospital of Chongqing Medical University, Chongqing, China.
authors: Hong-Bing
authors: HB
authors: Yao
authors: Department of Otolaryngology, The Children's Hospital of Chongqing Medical University, Chongqing, China.
authors: Wei
authors: W
authors: Kou
authors: Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors: Hong
authors: H
authors: Liu
authors: Department of Otolaryngology, The Children's Hospital of Chongqing Medical University, Chongqing, China.
authors: Cheng
authors: C
authors: Zhang
authors: Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors: Su-Ling
authors: SL
authors: Hong
mesh_terms: D016022:Case-Control Studies; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D004353:Drug Evaluation, Preclinical; D005260:Female; D006801:Humans; D007211:Indoles / Q000494:pharmacology / Q000627:therapeutic use*; D007378:Interleukins / Q000378:metabolism; D008297:Male; D018336:Receptors, Aryl Hydrocarbon / Q000819:agonists* / Q000378:metabolism; D065631:Rhinitis, Allergic; D012221:Rhinitis, Allergic, Perennial / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D058504:Th17 Cells / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D013844:Thiazoles / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: C548651:2-(1'H-indole-3'-carbonyl)thiazole-4-carboxylic acid methyl ester; D007211:Indoles; D007378:Interleukins; D018336:Receptors, Aryl Hydrocarbon; D013844:Thiazoles