title: Immunosuppressive effects of fisetin against dinitrofluorobenzene-induced atopic dermatitis-like symptoms in NC/Nga mice.
abstract: Atopic dermatitis (AD) is a multifactorial chronic skin disorder that is increasing in prevalence globally. In NC/Nga mice, repetitive epicutaneous applications of 2-4-dinitrofluorobenzene (DNFB) induces AD-like clinical symptoms. Bioflanonol fisetin (3,7,3',4'-tetrahydroxyflavone) is a dietary component found in plants, fruits and vegetables. Fisetin has various physiological effects that include anti-oxidation, anti-angiogenesis, anti-carcinogenesis and anti-inflammation. In this study, we investigated whether fisetin relieves AD-like clinical symptoms induced by repeated DNFB treatment in NC/Nga mice. Fisetin significantly inhibited infiltration of inflammatory cells including eosinophils, mast cells and CD4(+) T and CD8(+) T cells, and suppressed the expressions of cytokines and chemokines associated with dermal infiltrates in AD-like skin lesions. Total serum immunoglobulin E (IgE) levels and the ratio of phospho-NF-κB p65 to total NF-κB p65 were markedly reduced by fisetin. Fisetin also reduced the production of interferon-gamma and interleukin-4 by activated CD4(+) T cells in a dose-dependent manner, whereas the anti-inflammatory cytokine, interleukin-10 was increased. These results implicate fisetin as a potential therapeutic for AD.
authors: Department of Microbiology, School of Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors: Gun-Dong
authors: GD
authors: Kim
authors: Department of Microbiology, School of Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors: Seung Eun
authors: SE
authors: Lee
authors: Department of Microbiology, School of Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors: Yong Seek
authors: YS
authors: Park
authors: Department of Food & Biotechnology, College of Life Science, Korea University, Seoul 136-701, Republic of Korea.
authors: Dong-Hoon
authors: DH
authors: Shin
authors: Department of Food Science & Biotechnology, College of Life Science, Gachon University, Seongnam 461-701, Republic of Korea.
authors: Gwi Gun
authors: GG
authors: Park
authors: Department of Microbiology, School of Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea. Electronic address: pcs@khu.ac.kr.
authors: Cheung-Seog
authors: CS
authors: Park
mesh_terms: D000818:Animals; D001483:Base Sequence; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D017931:DNA Primers; D003876:Dermatitis, Atopic / Q000209:etiology*; D004139:Dinitrofluorobenzene / Q000633:toxicity*; D005419:Flavonoids / Q000494:pharmacology*; D044948:Flavonols; D007073:Immunoglobulin E / Q000097:blood; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008297:Male; D051379:Mice; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D005419:Flavonoids; D044948:Flavonols; D007166:Immunosuppressive Agents; D007073:Immunoglobulin E; D004139:Dinitrofluorobenzene; C017875:fisetin
keywords: 2-4-Dinitrofluorobenzene; Atopic dermatitis; Fisetin; NC/Nga; NF-κB