title: Age effects on blood gas, spirometry, airway reactivity, and bronchoalveolar lavage fluid cytology in clinically healthy horses.
abstract: BACKGROUND
  Despite the increasing number of geriatric horses attended by veterinarians, there is a lack of understanding of aging-related changes on the respiratory system of horses.
  
  
  OBJECTIVE
  To identify aging-related changes on the respiratory function and bronchoalveolar lavage fluid (BALF) cytology of horses.
  
  
  METHODS
  Fifteen healthy young adult (2-11 years) and 16 healthy aged (≥20 years) horses.
  
  
  METHODS
  The respiratory system was examined by measurement of arterial blood gases (ABG), use of respiratory inductive plethysmography (RIP) for assessment of breathing pattern and ventilatory parameters, histamine bronchoprovocation, and BALF cytology.
  
  
  RESULTS
  No significant differences were detected with regard to values obtained by ABG or bronchoprovocation of young adult and aged healthy horses. In aged horses, there were significant differences in mean ± SD of the following parameters when compared to young horses: prolonged expiratory time (Te) measured by RIP (3.9 ± 1.5 s versus 3.0 ± 0.6 s), decreased percentage of alveolar macrophages (40.6 ± 11.3% versus 53.5 ± 9.6%), and increased percentage of lymphocytes (53.4 ± 9.5% versus 43.9 ± 11.0%). No correlations between airway reactivity and ventilatory parameters, ABG, or BALF cytology were found in this asymptomatic population.
  
  
  CONCLUSIONS
  These results suggest that aging does not cause changes in the results obtained by ABG, most RIP-derived variables, and bronchoprovocation in the horse. A decreased percentage of macrophage and an increased percentage of lymphocytes in the BALF cytology may be expected in the asymptomatic geriatric horse and may be a result of aging.
authors: Department of Clinical Sciences, Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA.
authors: A P
authors: AP
authors: Pacheco
authors: M R
authors: MR
authors: Paradis
authors: A M
authors: AM
authors: Hoffman
authors: P
authors: P
authors: Hermida
authors: A
authors: A
authors: Sanchez
authors: J A
authors: JA
authors: Nadeau
authors: M
authors: M
authors: Tufts
authors: M R
authors: MR
authors: Mazan
mesh_terms: D000375:Aging / Q000097:blood / Q000502:physiology; D000818:Animals; D001784:Blood Gas Analysis / Q000662:veterinary; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D005260:Female; D006736:Horses / Q000097:blood / Q000254:growth & development / Q000502:physiology*; D008297:Male; D012143:Respiratory Physiological Phenomena; D013147:Spirometry / Q000662:veterinary
keywords: Aging in horses; Arterial blood gases; Geriatric; Pulmonary function testing