title: Antimicrobial synergism against different lineages of methicillin-resistant Staphylococcus aureus carrying SCCmec IV.
abstract: OBJECTIVE
  To evaluate the synergistic activity of antimicrobial drugs against lineages of methicillin-resistant Staphylococcus aureus (MRSA) carrying SCCmec IV. The biofilm production and related genes were also detected.
  
  
  RESULTS
  Forty two MRSA isolates were tested for biofilm production and related genes. Biofilm/biomass susceptibility to gentamicin (G), linezolid (L), rifampicin (R) and vancomycin (V) was determined for six isolates from three lineages prevalent in Rio de Janeiro hospitals in concentrations ranging from 0·25 to 64 μg ml(-1). Biomass was evaluated by microtitre plate test and number of viable cells (CFU cm(-2)) and inspected by epifluorescence microscopy. All isolates presented the icaA and sasG genes, but only 38% were biofilm producers. There were 50 and 45% biomass reductions when concentrations ≥4 μg ml(-1) of R or L and ≥16 μg ml(-1) of G or V, respectively, were used. Synergism tests produced a 55% biomass reduction with R(2μgml-1) + G(16μgml-1), R(2μgml-1) + L(2μgml-1), R(2μgml-1) + V(4μgml-1), and L(2μgml-1) + V(4μgml-1). Number of viable cells was reduced from 2 to 3 logs with R(2μgml-1) + L(2μgml-1) and R(2μgml-1) + V(4μgml-1).
  
  
  CONCLUSIONS
  Synergisms involving R plus L and R plus V caused important reductions in biofilm/biomass and the number of viable cells. Drug combinations should be considered in the chemotherapies of MRSA-SCCmec IV infections.
  
  
  CONCLUSIONS
  Biofilms in MRSA infections restrict the clinical choice of antimicrobials. Thus, knowledge of the best options for monotherapy and drug synergisms could improve clinical results.
authors: Department of Medical Microbiology, Institute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro, Nova Friburgo, Rio de Janeiro, Brazil.
authors: P D M
authors: PD
authors: de Matos
authors: S
authors: S
authors: Sedaca
authors: D C
authors: DC
authors: Ferreira
authors: N L
authors: NL
authors: Iorio
authors: V C S
authors: VC
authors: Toledo
authors: A I C
authors: AI
authors: Freitas
authors: F L
authors: FL
authors: Coelho
authors: C
authors: C
authors: Sousa
authors: K R N
authors: KR
authors: Dos Santos
authors: M O
authors: MO
authors: Pereira
mesh_terms: D000081:Acetamides / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D018533:Biomass; D004357:Drug Synergism; D005839:Gentamicins / Q000494:pharmacology; D006801:Humans; D000069349:Linezolid; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability; D023303:Oxazolidinones / Q000494:pharmacology; D012293:Rifampin / Q000494:pharmacology; D014640:Vancomycin / Q000494:pharmacology
chemical_list: D000081:Acetamides; D000900:Anti-Bacterial Agents; D005839:Gentamicins; D023303:Oxazolidinones; D014640:Vancomycin; D000069349:Linezolid; D012293:Rifampin
keywords: MRSA; SCC
  mec 
  IV; biofilm; biomass; drug synergism