title: Dietary restriction and fasting arrest B and T cell development and increase mature B and T cell numbers in bone marrow.
abstract: Dietary restriction (DR) delays ageing and extends life span. Both long- and short-term DR, as well as short-term fasting provide robust protection against many "neuronal and surgery related damaging phenomena" such as Parkinson's disease and ischemia-reperfusion injury. The exact mechanism behind this phenomenon has not yet been elucidated. Its anti-inflammatory actions prompted us to thoroughly investigate the consequences of DR and fasting on B and T cell compartments in primary and secondary lymphoid organs of male C57Bl/6 mice. In BM we found that DR and fasting cause a decrease in the total B cell population and arrest early B cell development, while increasing the number of recirculating mature B cells. In the fasting group, a significant reduction in peripheral B cell counts was observed in both spleen and mesenteric lymph nodes (mLN). Thymopoiesis was arrested significantly at double negative DN2 stage due to fasting, whereas DR resulted in a partial arrest of thymocyte development at the DN4 stage. Mature CD3(+) T cell populations were increased in BM and decreased in both spleen and mLN. Thus, DR arrests B cell development in the BM but increases the number of recirculating mature B cells. DR also arrests maturation of T cells in thymus, resulting in depletion of mature T cells from spleen and mLN while recruiting them to the BM. The functional relevance in relation to protection against organ damage needs to be determined.
authors: Department of Surgery, Division of Transplant Surgery, Erasmus MC-University Medical Center, Rotterdam, The Netherlands.
authors: Shushimita
authors: S
authors: Shushimita
authors: Department of Pulmonary Medicine, Erasmus MC-University Medical Center, Rotterdam, The Netherlands.
authors: Marjolein J W
authors: MJ
authors: de Bruijn
authors: Department of Surgery, Division of Transplant Surgery, Erasmus MC-University Medical Center, Rotterdam, The Netherlands.
authors: Ron W F
authors: RW
authors: de Bruin
authors: Department of Surgery, Division of Transplant Surgery, Erasmus MC-University Medical Center, Rotterdam, The Netherlands.
authors: Jan N M
authors: JN
authors: IJzermans
authors: Department of Pulmonary Medicine, Erasmus MC-University Medical Center, Rotterdam, The Netherlands.
authors: Rudi W
authors: RW
authors: Hendriks
authors: Department of Surgery, Division of Transplant Surgery, Erasmus MC-University Medical Center, Rotterdam, The Netherlands.
authors: Frank J M F
authors: FJ
authors: Dor
mesh_terms: D000818:Animals; D016175:B-Lymphocyte Subsets / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D001835:Body Weight; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D031204:Caloric Restriction; D002452:Cell Count; D059447:Cell Cycle Checkpoints; D002454:Cell Differentiation; D005215:Fasting; D016130:Immunophenotyping; D008221:Lymphoid Tissue / Q000166:cytology; D008297:Male; D051379:Mice; D010641:Phenotype; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000378:metabolism / Q000502:physiology*