title: Effect of fibrin sealant aided with Dexon mesh for renal repair in a rat model of partial nephrectomy.
abstract: BACKGROUND
  To evaluate the clinical efficacy and histochemical impact of a new technique of renal repair using a fibrin sealant and Dexon mesh in rats.
  
  
  METHODS
  Ten groups of Sprague-Dawley (SD) rats underwent a bilateral partial nephrectomy 30, 21, 14, 7 to 1 days before sacrifice. Renal repair was accomplished by suturing on one side and using fibrin sealant and Dexon mesh on the opposite side. The time for renal reconstruction was recorded for each approach and compared. In addition to histological evaluations, the isolated renal tissue studies included immunohistochemical analysis, and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  In comparison with suturing, renal repair using fibrin sealant and Dexon mesh was much faster. We demonstrated a significant attenuation of the initial inflammatory response in the fibrin-Dexon group. The specific alterations in transforming growth factor-β1 (Tgf-β1) mRNA expression were significantly lower in the fibrin-Dexon group.
  
  
  CONCLUSIONS
  The fibrin sealant and Dexon mesh significantly simplified the procedure by reducing the time of renal reconstruction. This approach can diminish the fibrotic reaction and offers a response for renal repair similar to the suturing technique.
authors: Division of Urology, Department of Surgery, Changhua Christian Hospital, Taiwan.
authors: Pai-Fu
authors: PF
authors: Wang
authors: School of Medicine, National Yang-Ming University, Taiwan.
authors: Allen W
authors: AW
authors: Chiu
authors: Department of Pathology, Changhua Christian Hospital, Taiwan.
authors: Yueh-Min
authors: YM
authors: Lin
authors: Division of Pediatric Nephrology, Children's Medical Center, China Medical University Hospital, Taiwan.
authors: Ching-Yuang
authors: CY
authors: Lin
authors: Division of Urology, Department of Surgery, Changhua Christian Hospital, Taiwan. Electronic address: jasta1206@gmail.com.
authors: Hung-Jen
authors: HJ
authors: Shih
mesh_terms: D000818:Animals; D005260:Female; D015718:Fibrin Tissue Adhesive; D007668:Kidney / Q000737:chemistry / Q000473:pathology / Q000601:surgery; D009392:Nephrectomy / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013526:Surgical Mesh; D053773:Transforming Growth Factor beta1 / Q000032:analysis
chemical_list: D015718:Fibrin Tissue Adhesive; D053773:Transforming Growth Factor beta1
keywords: Dexon mesh; Fibrin sealant; Partial nephrectomy