title: A mass spectrometry-based hybrid method for structural modeling of protein complexes.
abstract: We describe a method that integrates data derived from different mass spectrometry (MS)-based techniques with a modeling strategy for structural characterization of protein assemblies. We encoded structural data derived from native MS, bottom-up proteomics, ion mobility-MS and chemical cross-linking MS into modeling restraints to compute the most likely structure of a protein assembly. We used the method to generate near-native models for three known structures and characterized an assembly intermediate of the proteasomal base.
authors: Department of Chemistry, University of Oxford, South Parks Road, Oxford, United Kingdom.
authors: Argyris
authors: A
authors: Politis
authors: Department of Biology, Institute of Molecular Systems Biology, Eidgenossische Technische Hochschule (ETH) Zurich, Zurich, Switzerland.
authors: Florian
authors: F
authors: Stengel
authors: Department of Chemistry, University of Oxford, South Parks Road, Oxford, United Kingdom.
authors: Zoe
authors: Z
authors: Hall
authors: Department of Chemistry, University of Oxford, South Parks Road, Oxford, United Kingdom.
authors: Helena
authors: H
authors: Hernández
authors: Department of Biology, Institute of Molecular Systems Biology, Eidgenossische Technische Hochschule (ETH) Zurich, Zurich, Switzerland.
authors: Alexander
authors: A
authors: Leitner
authors: Department of Biology, Institute of Molecular Systems Biology, Eidgenossische Technische Hochschule (ETH) Zurich, Zurich, Switzerland.
authors: Thomas
authors: T
authors: Walzthoeni
authors: Department of Chemistry, University of Oxford, South Parks Road, Oxford, United Kingdom.
authors: Carol V
authors: CV
authors: Robinson
authors: Department of Biology, Institute of Molecular Systems Biology, Eidgenossische Technische Hochschule (ETH) Zurich, Zurich, Switzerland.|Faculty of Science, University of Zurich, Zurich, Switzerland.
authors: Ruedi
authors: R
authors: Aebersold
mesh_terms: D000818:Animals; D013058:Mass Spectrometry / Q000379:methods*; D008958:Models, Molecular; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins