title: How to build a time-gated luminescence microscope.
abstract: The sensitivity of filter-based fluorescence microscopy techniques is limited by autofluorescence background. Time-gated detection is a practical way to suppress autofluorescence, enabling higher contrast and improved sensitivity. In the past few years, three groups of authors have demonstrated independent approaches to build robust versions of time-gated luminescence microscopes. Three detailed, step-by-step protocols are provided here for modifying standard fluorescent microscopes to permit imaging time-gated luminescence.
authors: Advanced Cytometry Laboratories, MQ BioFocus Research Centre & Photonics Research Centre, Macquarie University, New South Wales, Australia.
authors: Dayong
authors: D
authors: Jin
authors: Advanced Cytometry Laboratories, MQ BioFocus Research Centre & Photonics Research Centre, Macquarie University, New South Wales, Australia.
authors: Yiqing
authors: Y
authors: Lu
authors: Newport Instruments, San Diego, California.
authors: Robert C
authors: RC
authors: Leif
authors: Newport Instruments, San Diego, California.
authors: Sean
authors: S
authors: Yang
authors: Department of Chemistry, University of Illinois at Chicago, Chicago, Illinois.
authors: Megha
authors: M
authors: Rajendran
authors: Department of Chemistry, University of Illinois at Chicago, Chicago, Illinois.
authors: Lawrence W
authors: LW
authors: Miller
mesh_terms: D002138:Calibration; D004655:Emulsions; D049449:Luminescence; D008853:Microscopy / Q000295:instrumentation*; D008863:Microspheres; D009821:Oils; D012815:Signal Processing, Computer-Assisted; D014867:Water
chemical_list: D004655:Emulsions; D009821:Oils; D014867:Water
keywords: autofluorescence; lanthanide; microscopy; time-gated luminescence