title: Sensory neuron development in mouse coccygeal vertebrae and its relationship to tail biopsies for genotyping.
abstract: A common method of genotyping mice is via tissue obtained from tail biopsies. However, there is no available information on the temporal development of sensory neurons in the tail and how their presence or absence might affect the age for performing tail biopsies. The goals of this study were to determine if afferent sensory neurons, and in particular nociceptive neurons, are present in the coccygeal vertebrae at or near the time of birth and if not, when they first can be visualized on or in those vertebrae. Using toluidine blue neuronal staining, transmission electron microscopy, and calcitonin-related gene peptide immunostaining, we found proximal to distal maturation of coccygeal nerve growth in the C57BL/6J mouse. Single nerve bundles were first seen on postpartum day (PPD) 0. On PPD 3 presumptive nociceptive sensory nerve fibers were seen entering the vertebral perichondrium. Neural development continued through the last time point (PPD 7) but at no time were neural fibers seen entering the body of the vertebrae. The effect of age on the development of pain perception in the neonatal mouse is discussed.
authors: Department of Animal Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America.
authors: Jerald
authors: J
authors: Silverman
authors: Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America.
authors: Gregory
authors: G
authors: Hendricks
mesh_terms: D000818:Animals; D001706:Biopsy / Q000379:methods; D005260:Female; D005838:Genotype; D051379:Mice; D008810:Mice, Inbred C57BL; D009412:Nerve Fibers / Q000502:physiology; D055495:Neurogenesis / Q000235:genetics* / Q000502:physiology*; D009619:Nociceptors / Q000502:physiology*; D012445:Sacrococcygeal Region / Q000502:physiology*; D011984:Sensory Receptor Cells / Q000502:physiology*; D013623:Tail / Q000294:innervation* / Q000502:physiology