title: Investigation into the cardiotoxic effects of doxorubicin on contractile function and the protection afforded by cyclosporin A using the work-loop assay.
abstract: Doxorubicin is known to cause cardiotoxicity through multiple routes including the build-up of reactive oxygen species and disruption of the calcium homeostasis in cardiac myocytes, but the effect of drug treatment on the associated biomechanics of cardiac injury remains unclear. Detecting and understanding the adverse effects of drugs on cardiac contractility is becoming a priority in non-clinical safety pharmacology assessment. The work-loop technique enables the assessment of force-length work-loop contractions, which mimic those of the pressure-volume work-loops experienced by the heart in vivo. During this study we evaluated whether the work-loop technique could potentially provide improved insight into the biomechanics associated with drug-induced cardiac dysfunction. In order to do this we investigated the cardiotoxic effects of doxorubicin and characterised the protection afforded by the co-administration of cyclosporin A (CsA). This study provides detailed biomechanical in vitro insight into the cardiac dysfunction associated with Doxorubicin treatment, including reduction in peak force, force during shortening and power output. These effects were significantly abrogated in doxorubicin-CsA co-treatment studies. Closely mimicking the in vivo pressure-volume muscle mechanics, this assay provides a quick and easy technique to gain a better understanding of the detailed biomechanics of drug-induced cardiac dysfunction.
authors: Department of Biomolecular and Sport Sciences, Coventry University, Cox Street, Coventry CV1 5FB, UK.
authors: Mayel
authors: M
authors: Gharanei
authors: Department of Biomolecular and Sport Sciences, Coventry University, Cox Street, Coventry CV1 5FB, UK.
authors: Afthab
authors: A
authors: Hussain
authors: Department of Biomolecular and Sport Sciences, Coventry University, Cox Street, Coventry CV1 5FB, UK.
authors: Rob S
authors: RS
authors: James
authors: Division of Biomedical Sciences, St. George's, University of London, Cranmer Terrace, Tooting, London SW17 0RE, UK.
authors: Omar
authors: O
authors: Janneh
authors: Department of Biomolecular and Sport Sciences, Coventry University, Cox Street, Coventry CV1 5FB, UK. Electronic address: h.maddock@coventry.ac.uk.
authors: Helen
authors: H
authors: Maddock
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity*; D001681:Biological Assay; D002316:Cardiotonic Agents / Q000494:pharmacology; D054715:Cardiotoxins / Q000633:toxicity*; D016572:Cyclosporine / Q000494:pharmacology; D004317:Doxorubicin / Q000633:toxicity*; D066298:In Vitro Techniques; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects*; D010210:Papillary Muscles / Q000187:drug effects* / Q000502:physiology; D017207:Rats, Sprague-Dawley
chemical_list: D000903:Antibiotics, Antineoplastic; D002316:Cardiotonic Agents; D054715:Cardiotoxins; D004317:Doxorubicin; D016572:Cyclosporine
keywords: Cardiotoxicity; CsA; Doxorubicin; Force; mPTP