title: Valencene oxidase CYP706M1 from Alaska cedar (Callitropsis nootkatensis).
abstract: (+)-Nootkatone is a natural sesquiterpene ketone used in grapefruit and citrus flavour compositions. It occurs in small amounts in grapefruit and is a major component of Alaska cedar (Callitropsis nootkatensis) heartwood essential oil. Upon co-expression of candidate cytochrome P450 enzymes from Alaska cedar in yeast with a valencene synthase, a C. nootkatensis valencene oxidase (CnVO) was identified to produce trans-nootkatol and (+)-nootkatone. Formation of (+)-nootkatone was detected at 144±10μg/L yeast culture. CnVO belongs to a new subfamily of the CYP706 family of cytochrome P450 oxidases.
authors: Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, 6708 PD Wageningen, The Netherlands; Plant Research International, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors: Katarina
authors: K
authors: Cankar
authors: Plant Research International, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors: Adèle
authors: A
authors: van Houwelingen
authors: Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, 6708 PD Wageningen, The Netherlands.
authors: Miriam
authors: M
authors: Goedbloed
authors: Division of Molecular and Computational Toxicology, Free University of Amsterdam, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands.
authors: Rokus
authors: R
authors: Renirie
authors: DSM Biotechnology Center, Alexander Fleminglaan 1, 2613 AX Delft, The Netherlands.
authors: René M
authors: RM
authors: de Jong
authors: Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, 6708 PD Wageningen, The Netherlands.
authors: Harro
authors: H
authors: Bouwmeester
authors: Plant Research International, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors: Dirk
authors: D
authors: Bosch
authors: Isobionics, Urmonderbaan 22, 6167 RD Geleen, The Netherlands.
authors: Theo
authors: T
authors: Sonke
authors: Plant Research International, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands. Electronic address: jules.beekwilder@wur.nl.
authors: Jules
authors: J
authors: Beekwilder
mesh_terms: D003001:Cloning, Molecular; D029779:Cupressaceae / Q000201:enzymology* / Q000235:genetics; D009251:NADPH-Ferrihemoprotein Reductase / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D000081223:Polycyclic Sesquiterpenes; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D012717:Sesquiterpenes / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D010940:Plant Proteins; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; D009251:NADPH-Ferrihemoprotein Reductase; C050302:nootkatone
keywords: (+)-Nootkatone; (+)-Valencene; Alaska cedar; Callitropsis nootkatensis; Cytochrome P450; Sesquiterpene