title: Effects of combined amendments on heavy metal accumulation in rice (Oryza sativa L.) planted on contaminated paddy soil.
abstract: Stabilization of heavy metals in situ was investigated. Two combined amendments (LS, limestone+sepiolite; HZ, hydroxyhistidine+zeolite) were applied at ratios of 0.2%, 0.4%, and 0.8% (w/w) to paddy soil with multi-metal (Pb, Cd, Cu, and Zn) contamination. The effects of these two combined amendments on heavy metal bioavailability in soil, and on uptake and accumulation of heavy metals in rice plants were investigated. Application of LS and HZ significantly increased soil pH values and cation exchange capacity contents, and resulted in a reduction in exchangeable fraction of metals and in extract metal concentrations of amended soils through toxicity characteristic leaching procedure (TCLP). LS and HZ obviously inhibited uptake and accumulation of Pb, Cd, Cu, and Zn in rice plants. Compared with the control soil, concentrations of Pb, Cd, Cu, and Zn in brown rice were decreased by 10.6-31.8%, 16.7-25.5%, 11.5-22.1%, and 11.7-16.3%, respectively, as a result of 0.2% to 0.8% addition of LS, and decreased by 5.1-40.8%, 16.7-20.0%, 8.1-16.2%, and 13.3-21.7%, respectively, as a result of 0.2-0.8% addition of HZ. Significant positive linear correlations were found between heavy metal concentrations in TCLP extracts and those in rice tissues except for Pb. Extracting heavy metals with TCLP was a more suitable method for estimating heavy metal bioavailability concentrations of amended soil than exchangeable fraction of heavy metals, because the latter underestimated heavy metal bioavailability. These results demonstrate that LS and HZ could be effective in reducing heavy metal bioavailability and accumulation in rice grown on multi-metal-contaminated soils.
authors: Institute of Environment Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China; College of Bioscience and Technology, Hunan Agricultural University, Changsha 410128, China.
authors: Hang
authors: H
authors: Zhou
authors: Institute of Environment Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China.
authors: Xin
authors: X
authors: Zhou
authors: Institute of Environment Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China.
authors: Min
authors: M
authors: Zeng
authors: Institute of Environment Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China. Electronic address: liaobh1020@163.com.
authors: Bo-Han
authors: BH
authors: Liao
authors: Institute of Environment Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China.
authors: Li
authors: L
authors: Liu
authors: Institute of Environment Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China.
authors: Wen-Tao
authors: WT
authors: Yang
authors: Institute of Environment Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China.
authors: Yan-Ming
authors: YM
authors: Wu
authors: Institute of Environment Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China.
authors: Qiong-Yao
authors: QY
authors: Qiu
authors: Institute of Environment Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China.
authors: Ying-Jie
authors: YJ
authors: Wang
mesh_terms: D006863:Hydrogen-Ion Concentration; D019216:Metals, Heavy / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D012275:Oryza / Q000737:chemistry / Q000378:metabolism*; D012987:Soil / Q000737:chemistry*; D012989:Soil Pollutants / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*
chemical_list: D019216:Metals, Heavy; D012987:Soil; D012989:Soil Pollutants
keywords: Bioavailability; Combined amendment; Heavy metals; Rice (Oryza sativa L.); Soil