title: Statistical optimization for production of chitin deacetylase from Rhodococcus erythropolis HG05.
abstract: A strain producing chitin deacetylase (CDA) was isolated and identified as Rhodococcus erythropolis by morphological characteristics and 16S rDNA analysis, named as R. erythropolis HG05. By Plackett-Burman and central composite design, CDA production from R. erythropolis HG05 was increased from 58.00 U/mL to 238.89 U/mL. With the crude enzyme from R. erythropolis HG05, the hydrolysate components from colloid chitin were chito-oligosaccharides with polymerization number larger than hexaose.
authors: Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, 59 Cangwu Road, Lianyungang 222005, China; Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China.
authors: Yuying
authors: Y
authors: Sun
authors: Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China. Electronic address: zhangjiquan@qdio.ac.cn.
authors: Jiquan
authors: J
authors: Zhang
authors: Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, 59 Cangwu Road, Lianyungang 222005, China.
authors: Shengjun
authors: S
authors: Wu
authors: Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, 59 Cangwu Road, Lianyungang 222005, China.
authors: Shujun
authors: S
authors: Wang
mesh_terms: D000581:Amidohydrolases / Q000096:biosynthesis*; D006868:Hydrolysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012240:Rhodococcus / Q000201:enzymology* / Q000235:genetics; D013499:Surface Properties
chemical_list: D012336:RNA, Ribosomal, 16S; D000581:Amidohydrolases; C019866:chitin deacetylase
keywords: Chitin deacetylase; Rhodococcus erythropolis; Statistical optimization