title: Pathogenecity of Pseudomonas aeruginosa in Oreochromis mossambicus and treatment using lime oil nanoemulsion.
abstract: Fish diseases caused by Pseudomonas aeruginosa, a known pathogenic organism, is responsible for considerable economic losses in the commercial cultivation of Oreochromis mossambicus (Tilapia). The bacteria were injected into healthy fish through intramuscular injection, oral and immersion challenge. Infection was confirmed by histopathological investigation of the infected organs. Lime nanoemulsion was prepared and the effectiveness of the nanoemulsion was studied both in vitro and in vivo by well diffusion assay and in vivo in the artificially infected fish. Results showed that the lime nanoemulsion was effective against the P. aeruginosa infection in O. mossambicus both in vitro and in vivo.
authors: Centre for Nanobiotechnology, VIT University, Vellore, Tamil Nadu, India.
authors: John
authors: J
authors: Thomas
authors: Centre for Nanobiotechnology, VIT University, Vellore, Tamil Nadu, India.
authors: S
authors: S
authors: Thanigaivel
authors: Centre for Nanobiotechnology, VIT University, Vellore, Tamil Nadu, India.
authors: S
authors: S
authors: Vijayakumar
authors: Centre for Nanobiotechnology, VIT University, Vellore, Tamil Nadu, India.
authors: Kuntal
authors: K
authors: Acharya
authors: Centre for Nanobiotechnology, VIT University, Vellore, Tamil Nadu, India.
authors: Dhairyasheel
authors: D
authors: Shinge
authors: Centre for Nanobiotechnology, VIT University, Vellore, Tamil Nadu, India.
authors: T Samuel Jeba
authors: TS
authors: Seelan
authors: Centre for Nanobiotechnology, VIT University, Vellore, Tamil Nadu, India.
authors: Amitava
authors: A
authors: Mukherjee
authors: Centre for Nanobiotechnology, VIT University, Vellore, Tamil Nadu, India. Electronic address: nchandrasekaran@vit.ac.in.
authors: Natarajan
authors: N
authors: Chandrasekaran
mesh_terms: D000818:Animals; D004655:Emulsions / Q000737:chemistry / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D049329:Nanostructures / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology*; D011552:Pseudomonas Infections / Q000188:drug therapy*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000472:pathogenicity; D017210:Tilapia / Q000382:microbiology*
chemical_list: D004655:Emulsions; D010938:Plant Oils
keywords: Histopathology; Lime oil nanoemulsion; Oreochromis mossambicus; Pathogenecity; Pseudomonas aeruginosa