title: Rapid generation of Epstein-Barr virus-specific T cells for cellular therapy.
abstract: Lymphoproliferative disease after hematopoietic stem cell transplantation is a serious and life-threatening disease. CD8(+) T lymphocytes play a key role in the control of viral diseases and some tumors. Adoptive immunotherapy has been shown to be effective in controlling and preventing some virus-related diseases in transplant recipients. However, the ex vivo production of cells for adoptive transfer is labor-intensive and expensive. To simplify the culture procedures of Epstein-Barr virus (EBV)-specific cytotoxic T lymphocytes, we explored a novel method, a rapid expansion protocol, that only used recombinant human interleukin to initiate the growth of clinical-grade EBV-specific T cells. After restimulation with EBV peptides principally from latent antigens and immediate/early antigens, the CD8(+) cells obtained could produce cytokines that had cytotoxic activity against target cells. This method provides a new possibility for the treatment of life-threatening EBV-associated malignancies.
authors: Department of Pediatrics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors: Q-F
authors: QF
authors: Hao
authors: Department of Pediatrics, Navy General Hospital, Beijing, China.
authors: Y-X
authors: YX
authors: Yang
authors: Department of Pediatrics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors: Y
authors: Y
authors: Wang
authors: Department of Pediatrics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors: L-M
authors: LM
authors: Chen
authors: Department of Pediatrics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors: G-Y
authors: GY
authors: Sheng
authors: Department of Pediatrics, Navy General Hospital, Beijing, China. Electronic address: kaishitengfei@163.com.
authors: Z
authors: Z
authors: Luan
mesh_terms: D019264:Adoptive Transfer / Q000379:methods; D001402:B-Lymphocytes / Q000166:cytology; D049109:Cell Proliferation; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D016207:Cytokines / Q000378:metabolism; D000939:Epitopes / Q000276:immunology; D020031:Epstein-Barr Virus Infections / Q000276:immunology*; D006680:HLA Antigens / Q000276:immunology; D004854:Herpesvirus 4, Human / Q000378:metabolism*; D006801:Humans; D016130:Immunophenotyping; D007167:Immunotherapy / Q000379:methods; D052118:Lysosomal-Associated Membrane Protein 1 / Q000378:metabolism; D033581:Stem Cell Transplantation; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
chemical_list: D016207:Cytokines; D000939:Epitopes; D006680:HLA Antigens; D052118:Lysosomal-Associated Membrane Protein 1