title: Rapid inactivation of proteins by knocksideways.
abstract: The knocksideways system inactivates proteins by using a small molecule to trap them onto mitochondria. It is typically ∼3 to 4 orders of magnitude faster than a knockdown. To get the best results out of a knocksideways, five parameters need to be optimized: the bait, the prey, the small molecule, the cell or organism, and the assay.
authors: Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom.
authors: Margaret S
authors: MS
authors: Robinson
authors: Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom.
authors: Jennifer
authors: J
authors: Hirst
mesh_terms: D000818:Animals; D001671:Biochemistry / Q000379:methods*; D019281:Dimerization; D006801:Humans; D008928:Mitochondria / Q000378:metabolism*; D051336:Mitochondrial Membranes / Q000378:metabolism*; D021381:Protein Transport; D020123:Sirolimus / Q000378:metabolism*; D022021:Tacrolimus Binding Proteins / Q000378:metabolism*; D013997:Time Factors
chemical_list: D022021:Tacrolimus Binding Proteins; D020123:Sirolimus
keywords: AP-1; AP-2; AP21967; clathrin; rapamycin