title: Mass spectrometric proteomics reveals that nuclear protein positive cofactor PC4 selectively binds to cross-linked DNA by a trans-platinum anticancer complex.
abstract: An MS-based proteomic strategy combined with chemically functionalized gold nanoparticles as affinity probes was developed and validated by successful identification and quantification of HMGB1, which is well characterized to interact selectively with 1,2-cross-linked DNA by cisplatin, from whole cell lysates. The subsequent application of this method to identify proteins responding to 1,3-cross-linked DNA by a trans-platinum anticancer complex, trans-PtTz (Tz = thiazole), revealed that the human nuclear protein positive cofactor PC4 selectively binds to the damaged DNA, implying that PC4 may play a role in cellular response to DNA damage by trans-PtTz.
authors: Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, PR China ;
authors: Zhifeng
authors: Z
authors: Du
authors: Qun
authors: Q
authors: Luo
authors: Liping
authors: L
authors: Yang
authors: Tao
authors: T
authors: Bing
authors: Xianchan
authors: X
authors: Li
authors: Wei
authors: W
authors: Guo
authors: Kui
authors: K
authors: Wu
authors: Yao
authors: Y
authors: Zhao
authors: Shaoxiang
authors: S
authors: Xiong
authors: Dihua
authors: D
authors: Shangguan
authors: Fuyi
authors: F
authors: Wang
mesh_terms: D045744:Cell Line, Tumor; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage / Q000187:drug effects*; D024243:HMGB1 Protein / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry; D053768:Metal Nanoparticles / Q000737:chemistry*; D009944:Organoplatinum Compounds / Q000737:chemistry*; D040901:Proteomics / Q000379:methods; D013844:Thiazoles / Q000737:chemistry*
chemical_list: D024243:HMGB1 Protein; D009944:Organoplatinum Compounds; D013844:Thiazoles; C504858:trans-(PtCl2(NH3)(thiazole)); D004247:DNA