title: Quantitative solid phase microextraction--gas chromatography mass spectrometry analysis of five megastigmatrienone isomers in aged wine.
abstract: Megastigmatrienone is a key flavor compound in tobacco. It has also been detected in wine, where it may contribute to a tobacco/incense aroma, but its importance and concentration in wines had never previously been evaluated. A method was developed and validated for quantifying the five megastigmatrienone isomers in red and white wines. Megastigmatrienone isomers were extracted by headspace solid-phase microextraction (HS-SPME), with a 65 μm film thickness polydimethylsiloxane-divinylbenzene (PDMS-DVB) fiber and analyzed using gas chromatography-mass spectrometry (GC/MS) in selected ion monitoring mode (SIM). Several parameters affecting the length of the adsorption process (i.e., adding salt, extraction time and extraction temperature) were tested. The optimum analytical conditions were established. The LOQ were between 0.06 μg L(-1) and 0.49 μg L(-1) for white wine and 0.11 μg L(-1) and 0.98 μg L(-1) for red wine, repeatability in both types of wine was less than 10% and recovery ranged from 96% for white wine to 94% for red wine. The five isomers of megastigmatrienone were quantified in red and white wines for the first time. Concentrations ranged from 2 μg L(-1) to 41 μg L(-1) in both red and white wines. Initial results revealed a link between wine aging and megastigmatrienone levels, indicating that megastigmatrienone may be a component in wine "bouquet".
authors: Univ. Bordeaux, ISVV, EA 4577, Unité de Recherche Œnologie, 33882 Villenave d'Ornon, France; INRA, ISVV, USC 1366 Œnologie, 33882 Villenave d'Ornon, France. Electronic address: davideslaghenaufi@gmail.com.
authors: Davide
authors: D
authors: Slaghenaufi
authors: Univ. Bordeaux, ISVV, EA 4577, Unité de Recherche Œnologie, 33882 Villenave d'Ornon, France; INRA, ISVV, USC 1366 Œnologie, 33882 Villenave d'Ornon, France.
authors: Marie-Claire
authors: MC
authors: Perello
authors: Univ. Bordeaux, ISVV, EA 4577, Unité de Recherche Œnologie, 33882 Villenave d'Ornon, France; INRA, ISVV, USC 1366 Œnologie, 33882 Villenave d'Ornon, France.
authors: Stéphanie
authors: S
authors: Marchand-Marion
authors: Univ. Bordeaux, ISVV, EA 4577, Unité de Recherche Œnologie, 33882 Villenave d'Ornon, France; INRA, ISVV, USC 1366 Œnologie, 33882 Villenave d'Ornon, France.
authors: Sophie
authors: S
authors: Tempere
authors: Univ. Bordeaux, ISVV, EA 4577, Unité de Recherche Œnologie, 33882 Villenave d'Ornon, France; INRA, ISVV, USC 1366 Œnologie, 33882 Villenave d'Ornon, France.
authors: Gilles
authors: G
authors: de Revel
mesh_terms: D003512:Cyclohexanones / Q000032:analysis*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007536:Isomerism; D057230:Limit of Detection; D011145:Polyvinyls / Q000737:chemistry; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction / Q000379:methods*; D014920:Wine / Q000032:analysis*
chemical_list: D003512:Cyclohexanones; D004129:Dimethylpolysiloxanes; D011145:Polyvinyls; C445089:poly(dimethylsiloxane-divinylbenzene); C549856:(4Z)-4-((E)-but-2-enylidene)-3,5,5-trimethylcyclohex- 2-en-1-one
keywords: Gas chromatography; Mass spectrometry; Megastigmatrienone; Solid phase microextraction; Tobacco aroma; Wine