title: High-resolution mass spectrometry applied to the identification of transformation products of quinolones from stability studies and new metabolites of enrofloxacin in chicken muscle tissues.
abstract: The aim of this work was the identification of new metabolites and transformation products (TPs) in chicken muscle from enrofloxacin (ENR), ciprofloxacin (CIP), difloxacin (DIF) and sarafloxacin (SAR), which are antibiotics that belong to the fluoroquinolones family. The stability of ENR, CIP, DIF and SAR standard solutions versus pH degradation process (from pH 1.5 to 8.0, simulating the pH since the drug is administered until its excretion) and freeze-thawing (F/T) cycles was tested. In addition, chicken muscle samples from medicated animals with ENR were analyzed in order to identify new metabolites and TPs. The identification of the different metabolites and TPs was accomplished by comparison of mass spectral data from samples and blanks, using liquid chromatography coupled to quadrupole time-of-flight (LC-QqToF) and multiple mass defect filter (MMDF) technique as a pre-filter to remove most of the background noise and endogenous components. Confirmation and structure elucidation was performed by liquid chromatography coupled to linear ion trap quadrupole Orbitrap (LC-LTQ-Orbitrap), due to its mass accuracy and MS/MS capacity for elemental composition determination. As a result, 21 TPs from ENR, 6 TPs from CIP, 14 TPs from DIF and 12 TPs from SAR were identified due to the pH shock and F/T cycles. On the other hand, 14 metabolites were identified from the medicated chicken muscle samples. Formation of CIP and SAR, from ENR and DIF, respectively, and the formation of desethylene-quinolone were the most remarkable identified compounds.
authors: Department of Analytical Chemistry, Food and Nutrition Torribera Campus, University of Barcelona, Avda. Prat de la Riba, 171, Sta. Coloma de Gramenet, E-08921 Barcelona, Spain.
authors: F J
authors: FJ
authors: Morales-Gutiérrez
authors: Department of Analytical Chemistry, Food and Nutrition Torribera Campus, University of Barcelona, Avda. Prat de la Riba, 171, Sta. Coloma de Gramenet, E-08921 Barcelona, Spain.
authors: M P
authors: MP
authors: Hermo
authors: Department of Analytical Chemistry, Food and Nutrition Torribera Campus, University of Barcelona, Avda. Prat de la Riba, 171, Sta. Coloma de Gramenet, E-08921 Barcelona, Spain.
authors: J
authors: J
authors: Barbosa
authors: Department of Analytical Chemistry, Food and Nutrition Torribera Campus, University of Barcelona, Avda. Prat de la Riba, 171, Sta. Coloma de Gramenet, E-08921 Barcelona, Spain. Electronic address: dolores.barron@ub.edu.
authors: D
authors: D
authors: Barrón
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry; D002645:Chickens; D002853:Chromatography, Liquid / Q000379:methods; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009132:Muscles / Q000737:chemistry*; D015363:Quinolones / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D015363:Quinolones; D000077422:Enrofloxacin
keywords: Chicken muscle; High-resolution mass spectrometry; Metabolites; Quinolones; Transformation products