title: Proteomic Analysis of Intracellular and Membrane Proteins From Voriconazole-Resistant Candida glabrata.
abstract: OBJECTIVE
  The proteomic analysis of voriconazole resistant Candida glabrata strain has not yet been investigated. In this study, differentially expressed proteins of intracellular and membrane fraction from voriconazole-susceptible, susceptible dose-dependent (S-DD), resistant C. glabrata strains were compared with each other and several proteins were identified.
  
  
  METHODS
  The proteins of intracellular and membrane were isolated by disrupting cells with glass bead and centrifugation from voriconazole susceptible, S-DD, and resistant C. glabrata strains. The abundance of expressed proteins was compared using two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis and proteins showing continuous twofold or more increase or reduction of expression in resistant strains compared to susceptible and S-DD strain were analyzed by liquid chromatography/mass spectrometry-mass spectrometry method.
  
  
  RESULTS
  Of 34 intracellular proteins, 15 proteins showed expression increase or reduction (twofold or more). The identified proteins included regulation, energy production, carbohydrate transport, amino acid transport, and various metabolism related proteins. The increase of expression of heat shock protein 70 was found. Among membrane proteins, 12, 31 proteins showed expression increase or decrease in the order of susceptible, S-DD, and resistant strains. This expression included carbohydrate metabolism, amino acid synthesis, and response to stress-related proteins. In membrane fractions, the change of expression of 10 heat shock proteins was observed, and 9 heat shock protein 70 (Hsp70) showed the reduction of expression.
  
  
  CONCLUSIONS
  The expression of Hsp70 protein in membrane fraction is related to voriconazole resistant C. glabrata strains.
authors: Division of Antimicrobial Resistance, Korea National Institute of Health, Osong, Korea.
authors: Jae Il
authors: JI
authors: Yoo
authors: Division of Antimicrobial Resistance, Korea National Institute of Health, Osong, Korea.
authors: Hwa Su
authors: HS
authors: Kim
authors: Proteome Research Team, Korea Basic Science Institute, Daejeon, Korea.
authors: Chi Won
authors: CW
authors: Choi
authors: Division of Antimicrobial Resistance, Korea National Institute of Health, Osong, Korea.
authors: Jung Sik
authors: JS
authors: Yoo
authors: Division of Antimicrobial Resistance, Korea National Institute of Health, Osong, Korea.
authors: Jae Yon
authors: JY
authors: Yu
authors: Division of Antimicrobial Resistance, Korea National Institute of Health, Osong, Korea.
authors: Yeong Seon
authors: YS
authors: Lee
keywords: Candida glabrata; Hsp70 protein; proteomic analysis; voriconazole