title: Thioflavin T as a fluorescence light-up probe for G4 formation.
abstract: Thioflavin T (ThT) becomes fluorescent in the presence of the G-quadruplex structure such as that formed by the human telomeric motif. In this report, we extend and generalize these observations and show that this dye may be used as a convenient and specific quadruplex probe. In the presence of most, but not all, G4-forming sequences, we observed a large increase in ThT fluorescence emission, whereas the presence of control duplexes and single strands had a more limited effect on emission. This differential behavior allowed us to design a high-throughput assay to detect G4 formation. Hundreds of different oligonucleotides may be tested in parallel for G4 formation with a simple fluorescence plate reader. We applied this technique to a family of aptamers not previously recognized as G4-forming sequences and demonstrated that ThT fluorescence signal may be used to predict G4 formation.
authors: ARNA Laboratory, University of Bordeaux, F-33000 Bordeaux, France, INSERM U869, IECB, F-33600 Pessac, France and Department of Chemistry and Biochemistry, Swarthmore College, Swarthmore, PA 19081, USA.
authors: Amandine
authors: A
authors: Renaud de la Faverie
authors: Aurore
authors: A
authors: Guédin
authors: Amina
authors: A
authors: Bedrat
authors: Liliya A
authors: LA
authors: Yatsunyk
authors: Jean-Louis
authors: JL
authors: Mergny
mesh_terms: D052160:Benzothiazoles; D005456:Fluorescent Dyes / Q000737:chemistry*; D054856:G-Quadruplexes; D063347:Native Polyacrylamide Gel Electrophoresis; D013844:Thiazoles / Q000737:chemistry*
chemical_list: D052160:Benzothiazoles; D005456:Fluorescent Dyes; D013844:Thiazoles; C009462:thioflavin T