title: Efficient secretion of (R)-3-hydroxybutyric acid from Halomonas sp. KM-1 by nitrate fed-batch cultivation with glucose under microaerobic conditions.
abstract: To establish a sustainable society, commodity chemicals need to be developed from biomass resources. Recently, (R)-3-hydroxybutyric acid ((R)-3-HB), a monomer of bioplastic poly-(R)-3-hydroxybutyric acid (PHB), has attracted attention for its possible use in the chemical industry. Halophilic bacteria have been considered for bioprocess applications due to certain characteristics such as the ability to grow in media containing high levels of the starting carbon source and the ability to be rarely contaminated. A halophilic bacterium Halomonas sp. KM-1 stores PHB intracellularly under aerobic conditions and secretes (R)-3-HB under microaerobic conditions. In this study, we optimized culture conditions to maximize (R)-3-HB secretion by KM-1 cells. By a simple nitrate fed-batch cultivation, Halomonas sp. KM-1 secreted 40.3g/L (R)-3-HB with a productivity of 0.48g L(-1)h(-1) with 20% (w/v) glucose. This level is one of the highest recorded productivity of (R)-3-HB to date.
authors: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan. Electronic address: y-kawata@aist.go.jp.
authors: Yoshikazu
authors: Y
authors: Kawata
authors: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan.
authors: Hitoshi
authors: H
authors: Ando
authors: Energy Technology Laboratories, OSAKA GAS CO., LTD., 6-19-9 Torishima, Konohana-ku, Osaka 554-0051, Japan.
authors: Isao
authors: I
authors: Matsushita
authors: Energy Technology Laboratories, OSAKA GAS CO., LTD., 6-19-9 Torishima, Konohana-ku, Osaka 554-0051, Japan.
authors: Jun
authors: J
authors: Tsubota
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000378:metabolism*; D000332:Aerobiosis / Q000187:drug effects; D061249:Batch Cell Culture Techniques / Q000379:methods*; D005947:Glucose / Q000494:pharmacology*; D020632:Halomonas / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D009566:Nitrates / Q000494:pharmacology*
chemical_list: D009566:Nitrates; D005947:Glucose; D020155:3-Hydroxybutyric Acid
keywords: (R)-3-Hydroxybutyric acid; Fed-batch cultivation; Halomonas; Nitrate; Poly-(R)-3-hydroxybutyric acid (PHB)