title: A review of reagents for fluorescence microscopy of cellular compartments and structures, Part II: reagents for non-vesicular organelles.
abstract: A wide range of fluorescent dyes and reagents exist for labeling organelles in live and fixed cells. Choosing between them can sometimes be confusing, and optimization for many of them can be challenging. Presented here is a discussion on the commercially-available reagents that have shown the most promise for each organelle of interest, including endoplasmic reticulum/nuclear membrane, Golgi apparatus, mitochondria, nucleoli, and nuclei, with an emphasis on localization of these structures for microscopy. Included is a featured reagent for each structure with a recommended protocol, troubleshooting guide, and example image.
authors: Molecular Probes Labeling and Detection, Life Technologies, Eugene, Oregon.
authors: Jason A
authors: JA
authors: Kilgore
authors: Molecular Probes Labeling and Detection, Life Technologies, Eugene, Oregon.
authors: Nick J
authors: NJ
authors: Dolman
authors: National High Magnetic Field Laboratory and Department of Biological Science, Florida State University, Tallahassee, Florida.
authors: Michael W
authors: MW
authors: Davidson
mesh_terms: D000818:Animals; D002451:Cell Compartmentation; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D007202:Indicators and Reagents; D008856:Microscopy, Fluorescence / Q000379:methods*; D015388:Organelles / Q000378:metabolism*; D013194:Staining and Labeling
chemical_list: D005456:Fluorescent Dyes; D007202:Indicators and Reagents
keywords: cell biology; fluorescent dyes; imaging; labeling; organelles