title: [Influence of zoledronate on osteoclast differentiation and gene expression of calmodulin and calmodulin-dependent protein kinase II].
abstract: OBJECTIVE
  To investigate the effect of zoledronate acid on osteoclast differentiation and gene expression of calmodulin (CAM) and calmodulin-dependent protein kinase (CAMK)II.
  
  
  METHODS
  Receptor activation of nuclear factor κB ligand (RANKL) was used to induce differentiation of RAW264.7 cells into osteoclasts in vitro. The cells were divided into two groups, group A and group B. Both groups were treated with RANKL for 5 days, whereas group B was also treated with zoledronate for the last 2 days.Osteoclastogenesis and gene expression of CAM and CAMK II were examined.
  
  
  RESULTS
  In group B, the number of new-generated osteoclasts (≥3 nuclei), number and size of dentin resorption lacunaes were (23 ± 3) , (19 ± 2) and (4951 ± 223) µm(2) respectively, which were significantly lower than those [(44 ± 3) , (46 ± 1) and (13 331 ± 248) µm(2)] in group A (P < 0.01).mRNA and protein level of CAM and CAMK II were also significantly down-regulated in group B when compared with group A (P < 0.01) and the decrease was 26.7% and 37.2% respectively for CAM, 57.0% and 76.1% respectively for CAMK II.
  
  
  CONCLUSIONS
  Zoledronate acid could significantly inhibit formation and resorption function of osteoclasts. CAM and CAMKII may be involved in the inhibition process.
authors: Department of Oral and Maxillofacial Surgery, College of Stomatology, Hebei United University, Tangshan Hebei 063000, China.
authors: Peng
authors: P
authors: Li
authors: Department of Oral and Maxillofacial Surgery, College of Stomatology, Hebei United University, Tangshan Hebei 063000, China.
authors: Jue-shan
authors: JS
authors: Lin
authors: Department of Oral and Maxillofacial Surgery, College of Stomatology, Hebei United University, Tangshan Hebei 063000, China.
authors: Peng
authors: P
authors: Zhang
authors: Department of Oral and Maxillofacial Surgery, College of Stomatology, Hebei United University, Tangshan Hebei 063000, China.
authors: Wei
authors: W
authors: Dong
authors: Department of Oral and Maxillofacial Surgery, College of Stomatology, Hebei United University, Tangshan Hebei 063000, China.
authors: Jin-yuan
authors: JY
authors: Li
authors: Department of Oral and Maxillofacial Surgery, College of Stomatology, Hebei United University, Tangshan Hebei 063000, China. Email: qimengchun@163.com.
authors: Meng-chun
authors: MC
authors: Qi
mesh_terms: D000818:Animals; D050071:Bone Density Conservation Agents / Q000494:pharmacology; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000235:genetics / Q000378:metabolism*; D002147:Calmodulin / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D002460:Cell Line; D004164:Diphosphonates / Q000494:pharmacology*; D007093:Imidazoles / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D051379:Mice; D010010:Osteoclasts / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D053246:Receptor Activator of Nuclear Factor-kappa B / Q000494:pharmacology; D000077211:Zoledronic Acid
chemical_list: D050071:Bone Density Conservation Agents; D002147:Calmodulin; D004164:Diphosphonates; D007093:Imidazoles; D012333:RNA, Messenger; D053246:Receptor Activator of Nuclear Factor-kappa B; D000077211:Zoledronic Acid; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2