title: Human platelet-rich plasma induces chondrogenic differentiation of subchondral progenitor cells in polyglycolic acid-hyaluronan scaffolds.
abstract: Cartilage repair approaches may be improved by addition of human platelet-rich plasma (PRP) that increases chondrogenic differentiation of mesenchymal stem and progenitor cells. The aim of our study was to evaluate the effect of human PRP on the differentiation of multipotent human subchondral progenitor cells in resorbable polyglycolic acid-hyaluronan (PGA-HA) scaffolds. PGA-HA scaffolds were loaded with subchondral progenitor cells and stimulated with transforming growth factor-beta3 (TGFB3) or 5% PRP, whereas nonstimulated cultures served as controls. Chondrogenic differentiation was evaluated by real-time gene expression analysis of typical chondrogenic marker genes and by immunohistochemical staining of extracellular cartilage matrix molecules such as proteoglycans and collagen type II. TGFB3 and PRP induced the expression of chondrogenic marker genes collagen type II and IX, aggrecan, and cartilage oligomeric matrix protein in subchondral progenitor cells cultured in PGA-HA scaffolds compared with nonstimulated controls. Progenitor cells in PGA-HA scaffolds formed an extracellular matrix rich in proteoglycans and collagen type II on treatment with PRP, but to a lesser extent, than in cultures stimulated with TGFB3. The results suggest that PRP induces chondrogenic differentiation of progenitor cells in PGA-HA scaffolds and may be therefore beneficial in scaffold-assisted cartilage repair approaches involving stem and progenitor cells.
authors: TransTissue Technologies GmbH, Charitéplatz 1, 10117, Berlin, Germany.
authors: Jan Philipp
authors: JP
authors: Krüger
authors: Anna-Katharina
authors: AK
authors: Ketzmar
authors: Michaela
authors: M
authors: Endres
authors: Axel
authors: A
authors: Pruss
authors: Alberto
authors: A
authors: Siclari
authors: Christian
authors: C
authors: Kaps
mesh_terms: D017667:Adipocytes / Q000166:cytology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology*; D020219:Chondrogenesis; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D006820:Hyaluronic Acid; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology; D053657:Platelet-Rich Plasma / Q000502:physiology*; D011100:Polyglycolic Acid; D020133:Reverse Transcriptase Polymerase Chain Reaction; D054457:Tissue Scaffolds; D053782:Transforming Growth Factor beta3 / Q000494:pharmacology
chemical_list: D016326:Extracellular Matrix Proteins; D053782:Transforming Growth Factor beta3; D011100:Polyglycolic Acid; D006820:Hyaluronic Acid
keywords: cartilage regeneration; platelet-rich plasma; scaffold; stem and progenitor cells; transforming growth factor-beta