title: Effect of bone marrow mesenchymal stem cells on the Smad expression of hepatic fibrosis rats.
abstract: OBJECTIVE
  To investigate the impact of bone marrow mesenchymal stem cells on Smad expression of hepatic fibrosis rats.
  
  
  METHODS
  A total of 48 adult female SD rats were randomly divided into three groups, normal control group (n=10), observation group (n=19) with liver fibrosis model rats injected with BMSCs cells; model group (n=19), with liver fibrosis model rats injected with physiological saline. Serum index, TGF-β1 and Smad expression were detected.
  
  
  RESULTS
  Type III procollagen, IV collagen, hyaluronic acid, laminin levels of observation group were significantly lower than those of model group (P<0.05). The content and expression of TGF-β1 in serum and liver tissue of observation group were significantly lower than those of model group(P<0.05). Compared with normal control group, the Smad3, Smad4 mRNA and protein expression of model group were significantly increased, the Smad7 mRNA and protein expression were significantly reduced (P<0.05). Compared with model group, Smad3, Smad4 mRNA and protein expression of observation group were significantly reduced, and Smad7 mRNA expression were significantly increased (P<0.05).
  
  
  CONCLUSIONS
  BMSCs can regulate Smad expression to some extent, and reduce the degree of liver fibrosis.
authors: Liver Disease Center, First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, Naning 530023, China. Electronic address: wangzhenchang924@163.com.
authors: Zhen-Chang
authors: ZC
authors: Wang
authors: Guangxi University of Traditional Chinese Medicine, Nanning 530007, China.
authors: Shan
authors: S
authors: Yang
authors: Liver Disease Center, First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, Naning 530023, China.
authors: Jing-Jing
authors: JJ
authors: Huang
authors: Liver Disease Center, First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, Naning 530023, China.
authors: Song-Lin
authors: SL
authors: Chen
authors: Guangxi University of Traditional Chinese Medicine, Nanning 530007, China.
authors: Quan-Qiang
authors: QQ
authors: Li
authors: Liver Disease Center, First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, Naning 530023, China.
authors: Yuan
authors: Y
authors: Li
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D003094:Collagen / Q000378:metabolism; D005260:Female; D006820:Hyaluronic Acid / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D008103:Liver Cirrhosis / Q000378:metabolism* / Q000473:pathology / Q000601:surgery*; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells; D051381:Rats; D017207:Rats, Sprague-Dawley; D051785:Smad Proteins / Q000032:analysis / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D007797:Laminin; D051785:Smad Proteins; D016212:Transforming Growth Factor beta; D006820:Hyaluronic Acid; D003094:Collagen
keywords: Bone mesenchymal stem cells; Hepatic fibrosis rats; Smadv