title: A dual amplification strategy for ultrasensitive electrochemiluminescence immunoassay based on a Pt nanoparticles dotted graphene-carbon nanotubes composite and carbon dots functionalized mesoporous Pt/Fe.
abstract: A facile and sensitive electrochemiluminescence (ECL) immunosensor for the detection of human carcinoembryonic antigen (CEA) was designed. The immunosensor used Pt nanoparticles dotted graphene-carbon nanotubes composites (Pt/Gr-CNTs) as a platform and carbon dots functionalized Pt/Fe nanoparticles (Pt/Fe@CDs) as bionanolabels. The Pt/Gr-CNTs was first synthesized using a facile ultrasonic method to modify the working electrode, which increases the surface area to capture a large amount of primary anti-CEA antibodies as well as improving the electronic transmission rate. The bionanolabels Pt/Fe@CDs prepared through ethanediamine linking, showed good ECL signal amplification performance. The reason is that the Pt/Fe@CDs nanocomposites as signal tags can increase CDs loading per immunoreaction in comparison with single CDs. The approach provided a good linear response range from 0.003 to 600 ng mL(-1) with a low detection limit of 0.8 pg mL(-1). The immunosensor showed good specificity, acceptable stability and reproducibility. Satisfactory results were obtained in the determination of CEA in human serum albumin samples. Hence, the proposed ECL immunosensor could become a promising method for tumor marker detection.
authors: Key Laboratory of Chemical Sensing & Analysis in the University of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China. chm_yanm@ujn.edu.cn.
authors: Wenping
authors: W
authors: Deng
authors: Fang
authors: F
authors: Liu
authors: Shenguang
authors: S
authors: Ge
authors: Jinghua
authors: J
authors: Yu
authors: Mei
authors: M
authors: Yan
authors: Xianrang
authors: X
authors: Song
mesh_terms: D000497:Alloys; D015374:Biosensing Techniques; D002272:Carcinoembryonic Antigen / Q000032:analysis; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D007501:Iron / Q000737:chemistry*; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*; D016062:Porosity; D045663:Quantum Dots / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000497:Alloys; D002272:Carcinoembryonic Antigen; D037742:Nanotubes, Carbon; D010984:Platinum; D006108:Graphite; D007501:Iron