title: Relationship between ischemia/reperfusion injury and acute rejection of allogeneic liver transplant in rats.
abstract: OBJECTIVE
  This study aimed to investigate the relationship between the severity of ischemia/reperfusion (I/R) injury and the acute rejection (AR) of allogeneic liver transplants in rats.
  
  
  METHODS
  The experimental rats were divided in different groups: normal control group (sham group, group I); syngeneic liver transplant control group (similar gene group, group II); and allogeneic liver transplant groups (groups III to VI). The rats were humanely killed at 1, 3, 5, and 7 days after transplantation or sham operation to determine the severity of I/R injury, rejection classification, and hepatocyte apoptosis. Messenger RNA (mRNA) and protein expression levels of Fas, perforin, and granzyme B were assessed in the liver tissues using real-time polymerase chain reaction and immunohistochemistry, respectively.
  
  
  RESULTS
  The rejection scores of the transplanted liver tissues gradually increased until these scores were proportional to the severity of I/R injury in groups III, IV, and V. The maximum scores were reached at 7 days after transplantation as the duration of transplantation was extended. The mRNA and protein expression levels of Fas, perforin, and granzyme B were significantly increased at 1, 3, 3, 5, and 7 days after liver reperfusion in groups III, IV, and V compared with those in groups I, II, and VI (P < .05).
  
  
  CONCLUSIONS
  The occurrence of AR after allogeneic liver transplantation in rats was positively correlated with the severity of I/R injury. Given that I/R injury caused serious damage to the transplanted liver, the occurrence of AR consequently decreased.
authors: Department of Hepatobiliary Surgery, Hunan Provincial People's Hospital, Changsha, Hunan Province, China.
authors: Y
authors: Y
authors: Wang
authors: Department of Hepatobiliary Surgery, Hunan Provincial People's Hospital, Changsha, Hunan Province, China. Electronic address: ygbzcn@126.com.
authors: J
authors: J
authors: Wu
authors: Department of Hepatobiliary Surgery, Hunan Provincial People's Hospital, Changsha, Hunan Province, China.
authors: B
authors: B
authors: Jiang
authors: Department of Hepatobiliary Surgery, Hunan Provincial People's Hospital, Changsha, Hunan Province, China.
authors: J
authors: J
authors: Wang
authors: Department of Hepatobiliary Surgery, Hunan Provincial People's Hospital, Changsha, Hunan Province, China.
authors: C
authors: C
authors: Liu
authors: Department of Hepatobiliary Surgery, Hunan Provincial People's Hospital, Changsha, Hunan Province, China.
authors: C
authors: C
authors: Peng
authors: Department of Hepatobiliary Surgery, Hunan Provincial People's Hospital, Changsha, Hunan Province, China.
authors: B
authors: B
authors: Tian
mesh_terms: D000818:Animals; D017209:Apoptosis; D001706:Biopsy; D004195:Disease Models, Animal; D005260:Female; D006084:Graft Rejection; D053804:Granzymes / Q000378:metabolism; D022781:Hepatocytes / Q000473:pathology; D006801:Humans; D008099:Liver / Q000473:pathology; D017093:Liver Failure / Q000601:surgery*; D016031:Liver Transplantation; D008297:Male; D052899:Pore Forming Cytotoxic Proteins / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology*; D019014:fas Receptor / Q000378:metabolism
chemical_list: C491898:Fas protein, rat; D052899:Pore Forming Cytotoxic Proteins; D019014:fas Receptor; C510465:perforin, rat; D053804:Granzymes; C509215:Gzmb protein, rat