title: Analysis of cell-free DNA in maternal blood in screening for aneuploidies: meta-analysis.
abstract: OBJECTIVE
  To review clinical validation or implementation studies of maternal blood cell-free (cf) DNA analysis in screening for aneuploidies and to explore the potential use of this method in clinical practice.
  
  
  METHODS
  Searches of PubMed and MEDLINE were performed to identify all peer-reviewed articles on cfDNA testing in screening for aneuploidies between 2011, when the first such study was published, and 20 December 2013.
  
  
  RESULTS
  Weighted pooled detection rates (DR) and false-positive rates (FPR) in singleton pregnancies were 99.0% (95% CI 98.2–99.6) and 0.08% (95% CI0.03–0.14), respectively, for trisomy 21; 96.8% (95% CI 94.5–98.4) and 0.15% (95% CI 0.08–0.25) for trisomy 18; 92.1% (95% CI 85.9–96.7) and 0.20% (95% CI 0.04–0.46) for trisomy 13; 88.6% (95% CI 83.0–93.1) and 0.12% (95% CI 0.05–0.24) for monosomy X, and 93.8% (95% CI 85.9–98.7) and 0.12% (95% CI 0.02–0.28) for sex chromosome aneuploidies other than monosomy X. For twin pregnancies, the DR was 94.4% (95% 74.2–99.0) and the FPR was 0% (95% CI 0.00–1.84) for trisomy 21.
  
  
  CONCLUSIONS
  An analysis of cfDNA in maternal blood provides effective screening for trisomies.
authors: M M
authors: MM
authors: Gil
authors: R
authors: R
authors: Akolekar
authors: M S
authors: MS
authors: Quezada
authors: B
authors: B
authors: Bregant
authors: K H
authors: KH
authors: Nicolaides
mesh_terms: D000782:Aneuploidy; D002474:Cell-Free System / Q000502:physiology; D004247:DNA / Q000235:genetics*; D005260:Female; D006801:Humans; D008423:Maternal Age; D062145:Maternal Serum Screening Tests / Q000379:methods / Q000592:standards*; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods / Q000592:standards; D014314:Trisomy / Q000235:genetics
chemical_list: D004247:DNA