title: Differential effects of aluminum on in vitro primary root growth, nutrient content and phospholipase C activity in coffee seedlings (Coffea arabica).
abstract: Coffea arabica is a woody species that grows in acid soils, where aluminum is available and may affect growth and productivity. To determine the effect of aluminum on primary root growth of C. arabica cv. Typica, seedlings were exposed over 30 days to different concentrations of AlCl3 (0, 100, 300 and 500 μM) in vitro. The aluminum effect on primary root growth was dose-dependent: low aluminum concentrations (100 and 300 μM) stimulated primary root growth (6.98 ± 0.15 and 6.45 ± 0.17 cm, respectively) compared to the control (0 μM; 5.24 ± 0.17 cm), while high concentrations (500 μM) induced damage to the root tips and inhibition of primary root growth (2.96 ± 0.28 cm). Aluminum (100 μM) also increased the K and Ca contents around 33% and 35% in the coffee roots. It is possible that aluminum toxicity resides in its association with cell nuclei in the meristematic region of the root. Additionally, after 30 days of treatment with aluminum, two different effects could be observed on phospholipase C (PLC) activity. In shoots, aluminum concentrations ≥ 300 μM inhibited more than 50% of PLC activity. In contrast, in roots a contrasting behavior was determined: low (100 μM) and toxic concentrations (500 μM) increased the activity of PLC (100%). These results suggest the possible involvement of the phosphoinositide signal transduction pathway, with the phospholipase C enzyme participating in the beneficial and toxic effects of aluminum in plants.
authors: Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43 # 130, Col. Chuburná de Hidalgo, 97200 Mérida, Yucatán, Mexico.
authors: Jesús E
authors: JE
authors: de A Bojórquez-Quintal
authors: Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43 # 130, Col. Chuburná de Hidalgo, 97200 Mérida, Yucatán, Mexico.
authors: Lucila A
authors: LA
authors: Sánchez-Cach
authors: Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43 # 130, Col. Chuburná de Hidalgo, 97200 Mérida, Yucatán, Mexico.
authors: Ángela
authors: Á
authors: Ku-González
authors: Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Calle 43 # 130, Col. Chuburná de Hidalgo, 97200 Mérida, Yucatán, Mexico.
authors: Cesar
authors: C
authors: de los Santos-Briones
authors: Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43 # 130, Col. Chuburná de Hidalgo, 97200 Mérida, Yucatán, Mexico.
authors: María
authors: M
authors: de Fátima Medina-Lara
authors: Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43 # 130, Col. Chuburná de Hidalgo, 97200 Mérida, Yucatán, Mexico.
authors: Ileana
authors: I
authors: Echevarría-Machado
authors: Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43 # 130, Col. Chuburná de Hidalgo, 97200 Mérida, Yucatán, Mexico.
authors: José A
authors: JA
authors: Muñoz-Sánchez
authors: Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43 # 130, Col. Chuburná de Hidalgo, 97200 Mérida, Yucatán, Mexico.
authors: S M
authors: SM
authors: Teresa Hernández Sotomayor
authors: Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43 # 130, Col. Chuburná de Hidalgo, 97200 Mérida, Yucatán, Mexico. Electronic address: luismanh@cicy.mx.
authors: Manuel Martínez
authors: MM
authors: Estévez
mesh_terms: D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000494:pharmacology*; D002712:Chlorides / Q000494:pharmacology*; D040503:Coffea / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D018517:Plant Roots / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D015398:Signal Transduction; D012964:Sodium / Q000378:metabolism; D010738:Type C Phospholipases / Q000378:metabolism*
chemical_list: D017607:Aluminum Compounds; D002712:Chlorides; D000077410:Aluminum Chloride; D012964:Sodium; D010738:Type C Phospholipases; D011188:Potassium
keywords: Aluminum; Coffee; Nutrient contents; PLC activity; Primary root growth