title: Staphylococcus aureus subsp. anaerobius strain ST1464 genome sequence.
abstract: Staphylococcus aureus subsp. anaerobius is responsible for Morel's disease in animals and a cause of abscess in humans. It is characterized by a microaerophilic growth, contrary to the other strains of S. aureus. The 2,604,446-bp genome (32.7% GC content) of S. anaerobius ST1464 comprises one chromosome and no plasmids. The chromosome contains 2,660 open reading frames (ORFs), 49 tRNAs and three complete rRNAs, forming one complete operon. The size of ORFs ranges between 100 to 4,600 bp except for two ORFs of 6,417 and 7,173 bp encoding segregation ATPase and non-ribosomal peptide synthase, respectively. The chromosome harbors Staphylococcus phage 2638A genome and incomplete Staphylococcus phage genome PT1028, but no detectable CRISPRS. The antibiotic resistance gene for tetracycline was found although Staphylococcus aureus subsp. anaerobius is susceptible to tetracycline in-vitro. Intact oxygen detoxification genes encode superoxide dismutase and cytochrome quinol oxidase whereas the catalase gene is impaired by a stop codon. Based on the genome, in-silico multilocus sequence typing indicates that S. aureus subsp. anaerobius emerged as a clone separated from all other S. aureus strains, illustrating host-adaptation linked to missing functions. Availability of S. aureus subsp. anaerobius genome could prompt the development of post-genomic tools for its rapid discrimination from S. aureus.
authors: Aix Marseille Université, URMITE, Marseille, France.
authors: Haitham
authors: H
authors: Elbir
authors: Aix Marseille Université, URMITE, Marseille, France.
authors: Catherine
authors: C
authors: Robert
authors: Aix Marseille Université, URMITE, Marseille, France.
authors: Ti Thien
authors: TT
authors: Nguyen
authors: Aix Marseille Université, URMITE, Marseille, France.
authors: Grégory
authors: G
authors: Gimenez
authors: Faculty of Veterinary Medicine, University of Khartoum, Sudan.
authors: Sulieman M
authors: SM
authors: El Sanousi
authors: Department of Microbiology, Tumor and Cell Biology, Stockholm, Sweden.
authors: Jan-Ingmar
authors: JI
authors: Flock
authors: Aix Marseille Université, URMITE, Marseille, France.
authors: Didier
authors: D
authors: Raoult
authors: Aix Marseille Université, URMITE, Marseille, France.
authors: Michel
authors: M
authors: Drancourt
keywords: Morel’s disease; SOLiD; Staphylococcus aureus subsp. anaerobius; genome