title: Polyvalent display of monosaccharides on ferritin protein cage nanoparticles for the recognition and binding of cell-surface lectins.
abstract: Carbohydrate-lectin interactions are important in many biological events. Endogenous cell-surface lectins are attractive markers for the recognition and targeting. Human ferritin protein cage nanoparticles (HFPCNs) are prepared as delivery nanoplatforms and two different types of monosaccharide derivatives; maleimido group terminated-mannopyranoside and galactopyranoside. Uniform and polyvalent displays of mannoses or galactoses on the surface of HFPCNs are achieved by using site-specific thiol-maleimide Michael-type addition. Mannose- or galactose-displaying HFPCNs recognize and tightly bind to DC-SIGN or ASGP-R lectins on the surface of the mammalian cells, DCEK or HepG2 cells.
authors: School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 689-798, Korea.
authors: Young Ji
authors: YJ
authors: Kang
authors: Hyun Ji
authors: HJ
authors: Yang
authors: Sangbin
authors: S
authors: Jeon
authors: Young-Sun
authors: YS
authors: Kang
authors: Yoonkyung
authors: Y
authors: Do
authors: Sung You
authors: SY
authors: Hong
authors: Sebyung
authors: S
authors: Kang
mesh_terms: D000818:Animals; D002460:Cell Line; D002850:Chromatography, Gel; D016503:Drug Delivery Systems / Q000379:methods*; D005293:Ferritins / Q000737:chemistry*; D006027:Glycosides / Q000138:chemical synthesis; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008856:Microscopy, Fluorescence; D008958:Models, Molecular; D015394:Molecular Structure; D009005:Monosaccharides / Q000737:chemistry / Q000378:metabolism*; D016296:Mutagenesis; D020349:Surface Plasmon Resonance
chemical_list: D006027:Glycosides; D037102:Lectins; D008565:Membrane Proteins; D009005:Monosaccharides; D005293:Ferritins
keywords: cell-surface lectins; delivery nanoplatforms; glycoconjugates; polyvalent display; protein cages