title: Antioxidant Chinese yam polysaccharides and its pro-proliferative effect on endometrial epithelial cells.
abstract: Chinese yam polysaccharide (CYP) was successfully isolated from the rhizoma of Dioscorea opposita and its chemical characteristics were determined. Antioxidant and pro-proliferative activity of CYP on human endometrial epithelial cells in vitro were investigated. Role of CYP on cell proliferation was also evaluated by examining pro-apoptotic and anti-apoptotic proteins Bax and Bcl-2 by Western blot analysis. Chemical composition analysis indicated that CYP was mainly composed of mannose, glucose, galactose and glucuronic acid in the ratio of 0.5:1.2:0.3:0.3. In vitro, CYP exhibited a potent scavenging activity on the DPPH radical, hydroxyl radical and superoxide radical. Furthermore, CYP apparently promoted the proliferation of human endometrial epithelial cells, especially beyond the concentration of 100 μg/ml after 36 h exposure. Western blot revealed that the anti-apoptotic protein Bcl-2 was upregulated after endometrial epithelial cells were treated with CYP, while the protein level of Bax was attenuated, thus leading to the downregulation of Bax/Bcl-2 ratio. Our findings provide the first evidence that CYP may prove to be a potential candidate of the natural antioxidants as a therapeutic agent for female infertility.
authors: Department of Obstetrics and Gynecology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors: Ying
authors: Y
authors: Ju
authors: Department of Preventive and Health Care, Shanxi People's hospital, Taiyuan 30053, China.
authors: Yan
authors: Y
authors: Xue
authors: Department of Obstetrics and Gynecology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors: Jianlei
authors: J
authors: Huang
authors: Department of Obstetrics and Gynecology, Chinese PLA General Hospital, Beijing 100853, China.
authors: Qingzhi
authors: Q
authors: Zhai
authors: Department of Obstetrics and Gynecology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710032, China. Electronic address: xiaohongwang_fmmu@tom.com.
authors: Xiao-Hong
authors: XH
authors: Wang
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D027742:Dioscorea / Q000737:chemistry*; D015536:Down-Regulation / Q000187:drug effects; D004717:Endometrium / Q000187:drug effects* / Q000378:metabolism; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D017665:Hydroxyl Radical / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D011134:Polysaccharides; D051028:bcl-2-Associated X Protein; D013481:Superoxides; D017665:Hydroxyl Radical
keywords: Antioxidant; Chinese yam polysaccharide; Endometrial epithelial cells; Female infertility; Female reproduction; Pro-proliferative