title: Denaturing gradient gel electrophoresis fingerprinting of soil bacteria in the vicinity of the Chinese Great Wall Station, King George Island, Antarctica.
abstract: Bacterial diversity was investigated in soil samples collected from 13 sites around the Great Wall Station, Fildes Peninsula, King George Island, Antarctica, using denaturing gradient gel electrophoresis (DGGE) of 16S rRNA genes. The classes alpha-, beta-, and gamma-Proteobacteria, as well as the phylum Actinobacteria, were found to be the dominant bacteria in the soils around the Great Wall Station. Although the selected samples were not contaminated by oil, a relationship between soil parameters, microbial biodiversity, and human impact was still seen. Sample sites in human impacted areas showed lower bacterial biodiversity (average H' = 2.65) when compared to non-impacted sites (average H' = 3.05). There was no statistically significant correlation between soil bacterial diversity and total organic carbon (TOC), total nitrogen, or total phosphorus contents of the soil. Canonical correlation analysis showed that TOC content was the most important factor determining bacterial community profiles among the measured soil parameters. In conclusion, microbial biodiversity and community characteristics within relatively small scales (1.5 km) were determined as a function of local environment parameters and anthropogenic impact.
authors: Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China. panqi021@gmail.com
authors: Qi
authors: Q
authors: Pan
authors: Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
authors: Feng
authors: F
authors: Wang
authors: Jinan Municipal Engineering Design and Research Institute Co., Ltd., Jinan 250101, China.
authors: Yang
authors: Y
authors: Zhang
authors: Key Laboratory for Polar Science of State Ocean Administration, Polar Research Institute of China, Shanghai 200136, China.
authors: Minghong
authors: M
authors: Cai
authors: Key Laboratory for Polar Science of State Ocean Administration, Polar Research Institute of China, Shanghai 200136, China.
authors: Jianfeng
authors: J
authors: He
authors: Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
authors: Haizhen
authors: H
authors: Yang
mesh_terms: D000864:Antarctic Regions; D001419:Bacteria / Q000145:classification* / Q000235:genetics; D001483:Base Sequence; D044822:Biodiversity; D016172:DNA Fingerprinting; D017931:DNA Primers; D058645:Denaturing Gradient Gel Electrophoresis; D006801:Humans; D012988:Soil Microbiology
chemical_list: D017931:DNA Primers