title: Increased expression of Tim-3 and its ligand Galectin-9 in rat allografts during acute rejection episodes.
abstract: The aim of the study is to elucidate the profiles of T-cell immunoglobulin and mucin domain-3 (Tim-3) and its ligand Galecin-9 in acute pulmonary rejection by using a rat model of lung transplantation. Left lung grafts retrieved from Lewis or Fisher 344 rats were orthotopically transplanted into Lewis recipients without any immunosuppressions; the grafts were harvested at day 3, 7 or 10 after transplantation. The grade of acute rejection was histopathologically evaluated. Tim-3, Galectin-9, immune antigen and related cytokines expression were assessed with immunological techniques and real-time polymerase chain reaction (RT-PCR), respectively. Then, our results showed that Tim-3 and its ligand Galectin-9 were markedly up-regulated at protein and mRNA levels in allografts compared with syngrafts. Meanwhile, the decreased CD4/CD8 ratio was associated with acute rejection occurring and Tim-3 expression on CD4(+) and CD8(+) T cells in allografts was increased. Therefore, our study firstly described that enhanced Tim-3 and its ligand Galectin-9 in allografts might play an important role in the pathogenesis of rat lung transplant rejection, implying new valuable markers for detecting acute allograft rejection.
authors: Division of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors: Xinwei
authors: X
authors: Qiao
authors: Division of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors: Ke
authors: K
authors: Jiang
authors: Division of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors: Jun
authors: J
authors: Nie
authors: Division of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors: Kai
authors: K
authors: Fan
authors: Division of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors: Zhikun
authors: Z
authors: Zheng
authors: Division of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address: bgjjww@yahoo.com.cn.
authors: Jianjun
authors: J
authors: Wang
authors: Division of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address: lijinsong67@hotmail.com.
authors: Jinsong
authors: J
authors: Li
mesh_terms: D064591:Allografts / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D000818:Animals; D016516:CD4-CD8 Ratio; D037161:Galectins / Q000032:analysis* / Q000235:genetics* / Q000276:immunology; D006084:Graft Rejection / Q000235:genetics* / Q000276:immunology / Q000473:pathology*; D016040:Lung Transplantation; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000235:genetics* / Q000276:immunology; D015854:Up-Regulation
chemical_list: D037161:Galectins; C569429:Havcr2 protein, rat; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; C513912:galectin 9, rat
keywords: Acute rejection; Galectin-9; Lung transplantation; Tim-3