title: Skin barrier integrity and natural moisturising factor levels after cumulative dermal exposure to alkaline agents in atopic dermatitis.
abstract: Dermal exposure to alkaline agents may lead to skin barrier damage and irritant contact dermatitis. The objective of this study was to investigate the effects of cumulative exposure to 0.5% sodium lauryl sulphate (SLS) and 0.15% NaOH on the barrier function and natural moisturising factor (NMF) levels in atopic dermatitis and healthy volunteers with known filaggrin genotype. The skin response was monitored by measurement of erythema and transepidermal water loss. The stratum corneum NMF levels were determined by high-performance liquid chromatography. Repeated exposure to 0.5% SLS and/or 0.15% NaOH in atopic dermatitis resulted in more severe impairment of the skin barrier function. Cumulative exposure to the irritants reduced significantly NMF in both the atopic and healthy controls group. The pronounced decrease of NMF after repeated single and sequential irritant exposure may be a pathogenetically relevant factor for development of chronic irritant contact dermatitis in both healthy and atopic individuals.
authors: Department of Dermatology, University of Lübeck, DE-23538 Lübeck, Germany. Irena.Angelova-Fischer@uk-sh.de.
authors: Irena
authors: I
authors: Angelova-Fischer
authors: Irena
authors: I
authors: Dapic
authors: Anne-Karin
authors: AK
authors: Hoek
authors: Ivone
authors: I
authors: Jakasa
authors: Tobias W
authors: TW
authors: Fischer
authors: Detlef
authors: D
authors: Zillikens
authors: Sanja
authors: S
authors: Kezic
mesh_terms: D000279:Administration, Cutaneous; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003876:Dermatitis, Atopic / Q000150:complications* / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism; D017453:Dermatitis, Irritant / Q000175:diagnosis / Q000209:etiology* / Q000378:metabolism; D004890:Erythema / Q000139:chemically induced / Q000175:diagnosis; D005260:Female; D000091344:Filaggrin Proteins; D020022:Genetic Predisposition to Disease; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007381:Intermediate Filament Proteins / Q000235:genetics; D007509:Irritants / Q000008:administration & dosage / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D010641:Phenotype; D012307:Risk Factors; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D023422:Skin Irritancy Tests; D012967:Sodium Dodecyl Sulfate / Q000008:administration & dosage / Q000009:adverse effects*; D012972:Sodium Hydroxide / Q000008:administration & dosage / Q000009:adverse effects*; D013997:Time Factors; D014867:Water / Q000378:metabolism*; D014870:Water Loss, Insensible / Q000187:drug effects*; D055815:Young Adult
chemical_list: C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins; D007509:Irritants; D014867:Water; D012967:Sodium Dodecyl Sulfate; D012972:Sodium Hydroxide