title: Regulation of LH/FSH expression by secretoglobin 3A2 in the mouse pituitary gland.
abstract: Secretoglobin (SCGB) 3A2 was originally identified as a downstream target for the homeodomain transcription factor NKX2-1 in the lung. NKX2-1 plays a role in the genesis and expression of genes in the thyroid, lung and ventral forebrain; Nkx2-1-null mice have no thyroid and pituitary and severely hypoplastic lungs and hypothalamus. To demonstrate whether SCGB3A2 plays any role in pituitary hormone production, NKX2-1 and SCGB3A2 expression in the mouse pituitary gland was examined by immunohistochemical analysis and RT-PCR. NKX2-1 was localized in the posterior pituitary lobe, whereas SCGB3A2 was observed in both anterior and posterior lobes as shown by immunohistochemistry and RT-PCR. Expression of CCAAT-enhancer binding proteins (C/EBPs), which regulate mouse Scgb3a2 transcription, was also examined by RT-PCR. C/EBPβ, γ, δ and ζ were expressed in the adult mouse pituitary gland. SCGB3A2 was expressed in the anterior and posterior lobes from postnatal days 1 and 5, respectively and the areas where SCGB3A2 expression was found coincided with the area where FSH-secreting cells were found. Double-staining for SCGB3A2 and pituitary hormones revealed that SCGB3A2 was mainly localized in gonadotrophs in 49 % of FSH-secreting cells and 47 % of LH-secreting cells. In addition, SCGB3A2 dramatically inhibited LH and FSH mRNA expression in rat pituitary primary cell cultures. These results suggest that SCGB3A2 regulates FSH/LH production in the anterior pituitary lobe and that transcription factors other than NKX2-1 may regulate SCGB3A2 expression.
authors: Biochemical Engineering, Faculty of Engineering, Yamagata University, Yonezawa, Yamagata, 992-8510, Japan.
authors: Yuki
authors: Y
authors: Miyano
authors: Shigeyuki
authors: S
authors: Tahara
authors: Ichiro
authors: I
authors: Sakata
authors: Takafumi
authors: T
authors: Sakai
authors: Hiroyuki
authors: H
authors: Abe
authors: Shioko
authors: S
authors: Kimura
authors: Reiko
authors: R
authors: Kurotani
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D005640:Follicle Stimulating Hormone / Q000378:metabolism*; D052681:Gonadotrophs / Q000378:metabolism; D007150:Immunohistochemistry; D007986:Luteinizing Hormone / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009687:Nuclear Proteins / Q000378:metabolism; D010902:Pituitary Gland / Q000166:cytology / Q000378:metabolism*; D010907:Pituitary Hormones / Q000378:metabolism; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D060765:Secretoglobins / Q000378:metabolism*; D013194:Staining and Labeling; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors / Q000378:metabolism
chemical_list: C000614414:Nkx2-1 protein, mouse; C000614416:Nkx2-1 protein, rat; D009687:Nuclear Proteins; D010907:Pituitary Hormones; D012333:RNA, Messenger; C449818:Scgb3a2 protein, mouse; D060765:Secretoglobins; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone