title: Generation and analysis of serine protease inhibitor kazal type 3-cre driver mice.
abstract: Serine protease inhibitor Kazal type 1 (SPINK1; mouse homologue Spink3) was initially discovered as a trypsin-specific inhibitor in the pancreas. However, previous studies have suggested that SPINK1/Spink3 is expressed in a wide range of normal tissues and tumors, although precise characterization of its gene expression has not been described in adulthood. To further analyze Spink3 expression, we generated two mouse lines in which either lacZ or Cre recombinase genes were inserted into the Spink3 locus by Cre-loxP technology. In Spink3(lacZ) mice, β-galactosidase activity was found in acinar cells of the pancreas and kidney, as well as epithelial cells of the bronchus in the lung, but not in the gastrointestinal tract or liver. Spink3(cre) knock-in mice were crossed with Rosa26 reporter (R26R) mice to monitor Spink3 promoter activity. In Spink3(cre);R26R mice, β-galactosidase activity was found in acinar cells of the pancreas, kidney, lung, and a small proportion of cells in the gastrointestinal tract and liver. These data suggest that Spink3 is widely expressed in endoderm-derived tissues, and that Spink3(cre) knock-in mice are a useful tool for establishment of a conditional knockout mice to analyze Spink3 function not only in normal tissues, but also in tumors that express SPINK1/Spink3.
authors: Institute of Resource Development and Analysis, Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto 860-0811, Japan.
authors: Kazuya
authors: K
authors: Sakata
authors: Masaki
authors: M
authors: Ohmuraya
authors: Kimi
authors: K
authors: Araki
authors: Chigure
authors: C
authors: Suzuki
authors: Satoshi
authors: S
authors: Ida
authors: Daisuke
authors: D
authors: Hashimoto
authors: Jung
authors: J
authors: Wang
authors: Yasuo
authors: Y
authors: Uchiyama
authors: Hideo
authors: H
authors: Baba
authors: Ken-Ichi
authors: K
authors: Yamamura
mesh_terms: D061354:Acinar Cells / Q000201:enzymology; D000818:Animals; D002460:Cell Line; D004847:Epithelial Cells / Q000201:enzymology; D015870:Gene Expression; D018014:Gene Transfer Techniques; D006023:Glycoproteins / Q000235:genetics* / Q000502:physiology; D019426:Integrases / Q000235:genetics*; D007668:Kidney / Q000166:cytology; D007763:Lac Operon / Q000235:genetics; D008168:Lung / Q000166:cytology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010179:Pancreas / Q000166:cytology; D011401:Promoter Regions, Genetic / Q000235:genetics; D029584:Prostatic Secretory Proteins / Q000235:genetics* / Q000502:physiology; D014359:Trypsin Inhibitor, Kazal Pancreatic; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D029584:Prostatic Secretory Proteins; C487134:Spink1 protein, mouse; D014359:Trypsin Inhibitor, Kazal Pancreatic; C045073:Cre recombinase; D019426:Integrases; D001616:beta-Galactosidase