title: Vaspin inhibits cytokine-induced nuclear factor-kappa B activation and adhesion molecule expression via AMP-activated protein kinase activation in vascular endothelial cells.
abstract: BACKGROUND
  Vaspin is an adipocytokine that was recently identified in the visceral adipose tissue of diabetic rats and has anti-diabetic and anti-atherogenic effects. We hypothesized that vaspin prevents inflammatory cytokine-induced nuclear factor-kappa B (NF-κB) activation by activating AMP-activated protein kinase (AMPK) in vascular endothelial cells.
  
  
  METHODS
  We examined the effects of vaspin on NF-κB activation and the expression of the NF-κB-mediated genes intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), E-selectin, and monocyte chemoattractant protein-1 (MCP-1). Human aortic endothelial cells (HAECS) were used. Tumor necrosis factor alpha (TNFα) was used as a representative proinflammatory cytokine.
  
  
  RESULTS
  Treatment with vaspin significantly increased the phosphorylation of AMPK and acetyl-CoA carboxylase, the down-stream target of AMPK. Furthermore, treatment with vaspin significantly decreased TNFα-induced activation of NF-κB, as well as the expression of the adhesion molecules ICAM-1, VCAM-1, E-selectin, and MCP-1. These effects were abolished following transfection of AMPKα1-specific small interfering RNA. In an adhesion assay using THP-1 cells, vaspin reduced TNFα-induced adhesion of monocytes to HAECS in an AMPK-dependent manner.
  
  
  CONCLUSIONS
  Vaspin might attenuate the cytokine-induced expression of adhesion molecule genes by inhibiting NF-κB following AMPK activation.
authors: Chang Hee
authors: CH
authors: Jung
authors: Min Jung
authors: MJ
authors: Lee
authors: Yu Mi
authors: YM
authors: Kang
authors: Yoo La
authors: YL
authors: Lee
authors: Hae Kyeong
authors: HK
authors: Yoon
authors: Sang-Wook
authors: SW
authors: Kang
authors: Woo Je
authors: WJ
authors: Lee
authors: Department of Internal Medicine, University of Ulsan College of Medicine, Poongnap-dong, Songpa-gu, Seoul 138-736, Korea. jypark@amc.seoul.kr.
authors: Joong-Yeol
authors: JY
authors: Park
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D002478:Cells, Cultured; D016207:Cytokines / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015843:Serpins / Q000494:pharmacology*
chemical_list: D015815:Cell Adhesion Molecules; D016207:Cytokines; D016328:NF-kappa B; C507339:SERPINA12 protein, human; D015843:Serpins; D055372:AMP-Activated Protein Kinases