title: Application of lipid extracts from Solidago canadensis to phytomonitoring of PCB126 in transgenic Arabidopsis plants.
abstract: The aim of this study is to elucidate the effect of lipid extracts from Solidago canadensis for phytomonitoring of polychlorinated biphenyl (PCB) 126 in the transgenic Arabidopsis plant XgD2V11-6 carrying the recombinant guinea pig (g) aryl hydrocarbon receptor (AhR)-mediated β-glucuronidase (GUS) reporter gene expression system. A lipid extract was prepared from S. canadensis and separated into simple lipid, glycolipid, and phospholipid fractions by silica gel column chromatography. Sterylglucoside (SG), monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), and glucosyl ceramide were found in the glycolipid fraction. When the transgenic Arabidopsis plants were treated with the glycolipid fraction together with PCB126, PCB126-induced GUS activity significantly increased in the whole plant. Moreover, S. canadensis-derived SG, MGDG, and DGDG also significantly increased PCB126-induced GUS activity. These results indicated that glycolipids in S. canadensis enhanced the sensitivity of this monitoring assay.
authors: Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan.
authors: Sayuri
authors: S
authors: Shimazu
authors: Department of Biotechnology, Fukuyama University, 1 Gakuen-cho, Fukuyama, Hiroshima 729-0292, Japan.
authors: Masaya
authors: M
authors: Ohta
authors: Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan. Electronic address: ashida@kobe-u.ac.jp.
authors: Hitoshi
authors: H
authors: Ashida
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000235:genetics*; D004784:Environmental Monitoring / Q000379:methods*; D038983:Galactolipids / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism; D006168:Guinea Pigs; D015386:Hazardous Substances / Q000633:toxicity*; D030821:Plants, Genetically Modified; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism; D036624:Solidago / Q000737:chemistry*
chemical_list: D038983:Galactolipids; D015386:Hazardous Substances; D018336:Receptors, Aryl Hydrocarbon; C007388:digalactosyldiacylglycerol; C009909:monogalactosyldiacylglycerol; D011078:Polychlorinated Biphenyls; D005966:Glucuronidase; C023035:3,4,5,3',4'-pentachlorobiphenyl
keywords: Arabidopsis thaliana; Aryl hydrocarbon receptor (AhR); Polychlorinated biphenyl (PCB); Solidago canadensis