title: Two homologs of rho-class and polymorphism in alpha-class glutathione S-transferase genes in the liver of three tilapias.
abstract: To clarify detoxification metabolism of tilapia, a natural and biological control for removing the leftover toxicants in fresh water, sequence structure, expression profile and polymorphisms of members of glutathione S-transferase (GST) genes were analyzed in Nile tilapia, blue tilapia and their hybrid. Full-length mRNA sequences of alpha-class GST (GSTA) and two homologs of rho-class GST (GSTR) were identified. Sequence analysis confirmed the similarity in conserved domain regions and their phylogenetic relationships with GST genes in other fishes. In addition, three single nucleotide polymorphisms of GSTA genes were identified in the three populations, two (C266T and G525A) of which showed significant association. The relative mRNA expression of GSTA gene was significantly (P<0.05) increased in the liver of Nile tilapia at 24h post-injection of MC-LR, significantly (P<0.05) decreased in blue tilapia whereas slightly decreased (P>0.05) in hybrid tilapia.
authors: Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, China.
authors: Ying
authors: Y
authors: Yu
authors: College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, China. Electronic address: xufang_liang@yahoo.com.
authors: Xu-Fang
authors: XF
authors: Liang
authors: College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, China.
authors: Ling
authors: L
authors: Li
authors: College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, China.
authors: Shan
authors: S
authors: He
authors: College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, China.
authors: Zheng-Yong
authors: ZY
authors: Wen
authors: College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, China.
authors: Dan
authors: D
authors: Shen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001427:Bacterial Toxins / Q000633:toxicity; D000087522:Cyanobacteria Toxins; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005982:Glutathione Transferase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008387:Marine Toxins / Q000633:toxicity; D052998:Microcystins / Q000633:toxicity; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D016415:Sequence Alignment; D017210:Tilapia / Q000145:classification / Q000235:genetics*; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; D008387:Marine Toxins; D052998:Microcystins; D014874:Water Pollutants, Chemical; D005982:Glutathione Transferase
keywords: Blue tilapia; Gene expression; Glutathione S-transferase; Hybrid tilapia; Nile tilapia; Single nucleotide polymorphism