title: An obesity-like gene MdTLP7 from apple (Malus × domestica) enhances abiotic stress tolerance.
abstract: Tubby-like proteins (TLPs) are found in a broad range of multicellular organisms. In mammals, genetic mutation of tubby or other TLPs can result in certain disease phenotypes related to animal specific characters: obesity, retinal degeneration, hearing loss, et al. Plants also harbor a large number of TLP genes, but the information in plants is far more limited. We identified a highly up-regulated obesity-like gene, MdTLP7, in our previous study of apple differential gene expression profile under chilling, indicating its possible role in plant abiotic stress tolerance. cDNA of MdTLP7 was amplified and expressed in Escherichia coli. In the solid and solution medium, the rate of growth and the quantity of the cell carrying MdTLP7 gene were significantly more than that of empty vector under salt and temperature stresses. To identify the functional region, serial deletion from both N-terminus and C-terminus of MdTLP7 was performed. In 415 amino acid polypeptide chain of MdTLP7, a middle conservative fragment (120-310 amino acid residues) played vital roles in stress tolerance. This fragment was involved in β barrel of Tubby domain according to the model of Tubby domain. All above results suggested MdTLP7 confers stress-tolerance to E. coli cell against abiotic stresses.
authors: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, People's Republic of China.
authors: Fan
authors: F
authors: Du
authors: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, People's Republic of China.
authors: Jia-Ning
authors: JN
authors: Xu
authors: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, People's Republic of China.
authors: Chun-Yan
authors: CY
authors: Zhan
authors: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, People's Republic of China.
authors: Zhi-Bo
authors: ZB
authors: Yu
authors: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, People's Republic of China; State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, Shandong 271018, People's Republic of China. Electronic address: xyunwang@sdau.edu.cn.
authors: Xiao-Yun
authors: XY
authors: Wang
mesh_terms: D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development* / Q000502:physiology; D015870:Gene Expression; D017343:Genes, Plant; D027845:Malus / Q000235:genetics*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D013312:Stress, Physiological
chemical_list: D010940:Plant Proteins; D011994:Recombinant Proteins
keywords: E. coli; Functional region; Stress tolerance; Tubby-like protein