title: Dynamics of the two heterochromatin types during imprinted X chromosome inactivation in vole Microtus levis.
abstract: In rodent female mammals, there are two forms of X-inactivation - imprinted and random which take place in extraembryonic and embryonic tissues, respectively. The inactive X-chromosome during random X-inactivation was shown to contain two types of facultative heterochromatin that alternate and do not overlap. However, chromatin structure of the inactive X-chromosome during imprinted X-inactivation, especially at early stages, is still not well understood. In this work, we studied chromatin modifications associated with the inactive X-chromosome at different stages of imprinted X-inactivation in a rodent, Microtus levis. It has been found that imprinted X-inactivation in vole occurs in a species-specific manner in two steps. The inactive X-chromosome at early stages of imprinted X-inactivation is characterized by accumulation of H3K9me3, HP1, H4K20me3, and uH2A, resembling to some extent the pattern of repressive chromatin modifications of meiotic sex chromatin. Later, the inactive X-chromosome recruits trimethylated H3K27 and acquires the two types of heterochromatin associated with random X-inactivation.
authors: Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia ; State Research Institute of Circulation Pathology, Novosibirsk, Russia ; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
authors: Evgeniya A
authors: EA
authors: Vaskova
authors: Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia ; State Research Institute of Circulation Pathology, Novosibirsk, Russia ; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
authors: Elena V
authors: EV
authors: Dementyeva
authors: Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia ; State Research Institute of Circulation Pathology, Novosibirsk, Russia ; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
authors: Alexander I
authors: AI
authors: Shevchenko
authors: Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia ; State Research Institute of Circulation Pathology, Novosibirsk, Russia ; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
authors: Sophia V
authors: SV
authors: Pavlova
authors: Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia ; State Research Institute of Circulation Pathology, Novosibirsk, Russia ; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
authors: Elena V
authors: EV
authors: Grigor'eva
authors: Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
authors: Antonina I
authors: AI
authors: Zhelezova
authors: Department of Genetics and Southwest National Primate Research Center, Texas Biomedical Research Institute, San Antonio, Texas, United States of America.
authors: John L
authors: JL
authors: Vandeberg
authors: Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia ; State Research Institute of Circulation Pathology, Novosibirsk, Russia ; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
authors: Suren M
authors: SM
authors: Zakian
mesh_terms: D000818:Animals; D003411:Arvicolinae / Q000235:genetics*; D047108:Embryonic Development / Q000235:genetics; D005260:Female; D018392:Genomic Imprinting / Q000235:genetics*; D006570:Heterochromatin / Q000235:genetics*; D006657:Histones / Q000235:genetics / Q000378:metabolism; D008540:Meiosis / Q000235:genetics; D012728:Sex Chromatin / Q000235:genetics; D013234:Stem Cells / Q000378:metabolism; D014327:Trophoblasts / Q000378:metabolism; D014960:X Chromosome / Q000235:genetics*; D049951:X Chromosome Inactivation / Q000235:genetics*; D014998:Y Chromosome / Q000235:genetics
chemical_list: D006570:Heterochromatin; D006657:Histones