title: Failure to detect functional neutrophil B helper cells in the human spleen.
abstract: A novel role for human neutrophilic granulocytes was recently described, showing that these cells, upon entering the spleen, can be reprogrammed into a distinct B cell-helper neutrophil phenotype that is capable of eliciting B cell responses such as immunoglobulin secretion, class switch recombination and somatic hypermutation. Using similar protocols, we detected a homogeneous population of CD15(high)CD16(high) neutrophils in fresh human spleen samples, which did not differ in phenotype and function from blood neutrophils. No phenotypic characteristics of costimulatory nature were detected on splenic or circulating neutrophils, nor could we reproduce the immunoglobulin production of splenic B cells in the presence of splenic neutrophils, although B cell function and neutrophil activity were normal. Independent confirmation of a role for NBH cells is required.
authors: Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, Amsterdam, The Netherlands.
authors: Sietse Quirijn
authors: SQ
authors: Nagelkerke
authors: Department of Pediatric Hematology, Immunology and Infectious Diseases, Emma Children's Hospital, Academic Medical Center, Amsterdam, The Netherlands ; Department of Experimental Immunology, Academic Medical Center, Amsterdam, The Netherlands.
authors: Daan Jacob
authors: DJ
authors: aan de Kerk
authors: Department of Pediatric Hematology, Immunology and Infectious Diseases, Emma Children's Hospital, Academic Medical Center, Amsterdam, The Netherlands ; Department of Experimental Immunology, Academic Medical Center, Amsterdam, The Netherlands.
authors: Machiel Hugo
authors: MH
authors: Jansen
authors: Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, Amsterdam, The Netherlands.
authors: Timo Kars
authors: TK
authors: van den Berg
authors: Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, Amsterdam, The Netherlands ; Department of Pediatric Hematology, Immunology and Infectious Diseases, Emma Children's Hospital, Academic Medical Center, Amsterdam, The Netherlands.
authors: Taco Willem
authors: TW
authors: Kuijpers
mesh_terms: D001402:B-Lymphocytes / Q000166:cytology*; D002454:Cell Differentiation; D002469:Cell Separation; D005434:Flow Cytometry; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology; D016256:Lewis X Antigen / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology*; D010641:Phenotype; D017382:Reactive Oxygen Species / Q000378:metabolism; D017452:Receptors, IgG / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000276:immunology*
chemical_list: D007136:Immunoglobulins; D016256:Lewis X Antigen; D017382:Reactive Oxygen Species; D017452:Receptors, IgG