title: [Study on a 905A to G mutation of α 1,3 galactosyltransferase gene].
abstract: OBJECTIVE
  To study the effect of 905A to G mutation of α -1,3 galactosyltransferase of ABO gene on B antigen expression.
  
  
  METHODS
  Three samples were diagnosed as B subgroup by serological test. Genotyping and sequencing were performed with polymerase chain reaction-sequence specific primer (PCR-SSP), direct sequencing and gene dones of exons 6 and 7 of the ABO locus.
  
  
  RESULTS
  The sequence of B allele has differed from that of regular B101 allele with a 905A to G missense mutation in exon 7, which resulted in an amino acid substitution (D302G) in all of the three B subgroup samples.
  
  
  CONCLUSIONS
  905A to G mutation can reduce the expression of B antigen.
authors: Liaoning Blood Center, Shenyang, Liaoning 110044, P.R.China. ljp_63@yahoo.com.
authors: Fengqiu
authors: F
authors: Lin
authors: Xu
authors: X
authors: Zhang
authors: Jianping
authors: J
authors: Li
mesh_terms: D000017:ABO Blood-Group System / Q000235:genetics; D000483:Alleles; D019943:Amino Acid Substitution; D001483:Base Sequence; D005260:Female; D005700:Galactosyltransferases / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D020125:Mutation, Missense
chemical_list: D000017:ABO Blood-Group System; D005700:Galactosyltransferases; C047006:N-acetyllactosaminide alpha-1,3-galactosyltransferase