title: Correlations of β-catenin, Ki67 and Her-2/neu with gastric cancer.
abstract: OBJECTIVE
  To study the clinical pathologic characteristics of β-catenin, Ki67 and Her-2/neu in gastric cancer and the correlation of β-catenin and Ki67 to the protein expression and gene conditions of Her-2/Neu.
  
  
  METHODS
  The protein expression of β-catenin, Ki67 and Her-2/Neu was detected by immunohistochemistry in 101 cases of gastric cancer and the gene conditions of Her-2/Neu by fluorescence in situ hybridization (FISH).
  
  
  RESULTS
  The protein expression of β-catenin, Ki67 and Her-2/Neu had close relationship with the clinical pathologic characteristics of gastric cancer. The β-catenin and Ki67 had obvious correlation to the differentiation, infiltration and lymphatic metastasis of the gastric cancer (P<0.05). The Ki67 had close relationship with the tumor-node-metastasis staging staging of gastric cancer (P<0.05). Her-2/Neu had close relationship with the differentiation and tumor-node-metastasis staging of gastric cancer (P<0.05) but had no relationship with the infiltration and lymphatic metastasis of the gastric cancer (P<0.05). The protein expression of Ki67 had significantly positive correlation to the protein expression and gene amplification conditions of Her-2/Neu (r=0.567, P<0.05 for protein; r=0.304, P<0.05 for gene).
  
  
  CONCLUSIONS
  Combined detection of β-catenin, Ki67 and Her-2/Neu can be used as a reliable method to help the observation of biological behavior, diagnosis and prognosis of gastric cancer, and Ki67 can be used to serve the preliminary screening of Her-2/Neu gene state.
authors: School of Medicine, Shandong University, Jinan 250012, China.
authors: Hong-Wen
authors: HW
authors: Wu
authors: Digestive Internal Medicine, The First Hospital of Zibo City, Zibo 255200, China. Electronic address: hongwenwu70@126.com.
authors: Cheng-Yong
authors: CY
authors: Qin
authors: Digestive Internal Medicine, Shandong Provincial Hospital, Jinan 250021, China.
authors: Ji-Lai
authors: JL
authors: Huang
authors: Digestive Internal Medicine, Shandong Provincial Hospital, Jinan 250021, China.
authors: Xian-Yi
authors: XY
authors: Kong
authors: Digestive Internal Medicine, Shandong Qianfoshan Hospital, Jinan 255200, China.
authors: Wen-Ji
authors: WJ
authors: Wang
authors: Digestive Internal Medicine, Shandong Qianfoshan Hospital, Jinan 255200, China.
authors: Wen-Kun
authors: WK
authors: Bai
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D019394:Ki-67 Antigen / Q000097:blood*; D008297:Male; D008875:Middle Aged; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000097:blood*; D018709:Statistics, Nonparametric; D013274:Stomach Neoplasms / Q000097:blood*; D051176:beta Catenin / Q000097:blood*
chemical_list: D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen; D018719:Erb-b2 Receptor Tyrosine Kinases; D051176:beta Catenin; C508053:ERBB2 protein, human
keywords: Fluorescence in situ hybridization; Gastric cancer; Her-2/neu; Immunohistochemistry; Ki67; Tumor marker; β-catenin