title: Optoelectronic tweezers system for single cell manipulation and fluorescence imaging of live immune cells.
abstract: A compact optoelectronic tweezers system for combined cell manipulation and analysis is presented. CMOS-controlled gallium nitride micro-LED arrays are used to provide simultaneous spatio-temporal control of dielectrophoresis traps within an optoelectronic tweezers device and fluorescence imaging of contrasting dye labelled cells. This capability provides direct identification, selection and controlled interaction of single T-lymphocytes and dendritic cells. The trap strength and profile for two emission wavelengths of micro-LED array have been measured and a maximum trapping force of 13.1 and 7.6 pN was achieved for projected micro-LED devices emitting at λmax 520 and 450 nm, respectively. A potential application in biological research is demonstrated through the controlled interaction of live immune cells where there is potential for this method of OET to be implemented as a compact device.
authors: Abigail H
authors: AH
authors: Jeorrett
authors: Steven L
authors: SL
authors: Neale
authors: David
authors: D
authors: Massoubre
authors: Erdan
authors: E
authors: Gu
authors: Robert K
authors: RK
authors: Henderson
authors: Owain
authors: O
authors: Millington
authors: Keith
authors: K
authors: Mathieson
authors: Martin D
authors: MD
authors: Dawson
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000295:instrumentation*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology* / Q000502:physiology*; D004581:Electronics / Q000295:instrumentation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D008846:Micromanipulation / Q000295:instrumentation; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D052898:Optical Tweezers; D013601:T-Lymphocytes / Q000166:cytology* / Q000502:physiology*