title: Establishment and identification of induced pluripotent stem cells in liver cancer patients.
abstract: OBJECTIVE
  To induce pluripotent stem (IPS) cells from fibrocytes that are separated from liver cancer patients.
  
  
  METHODS
  The fibrocytes were reprogrammed to IPS cells by lentiviral vector, stained and identified by immunohistochemistry.
  
  
  RESULTS
  The IPS cells were successfully established from fibrocytes after infection, and IPS cell clones formed in round shape under a microscopy. The induction rate was 0.013%±0.007%. No tumor formed at the back of nude mice within 8 weeks after the inoculation of cell clones. However, tetatoma appeared in nude mice within 1 week after IPS inoculation. A few tumors formed in nude mice within 4 weeks after the inoculation of cell clones. However, subcutaneous tumors formed within 1 week after IPS inoculation. The induced IPS cells showed three germ layers in tetatoma. Nanog and OCT4 in the induced IPS cells showed hypomethylation. SSEA-A, TRA-1-6-, TRA-1-81 and Nanog were highly expressed in the induced IPS cells, indicating the IPS cells possessed the similar ability as the stem cells.
  
  
  CONCLUSIONS
  The IPS cells of liver cancer patients can be established effectively from fibrocytes and can be cultured stably in vitro, which provides an approach for the treatment of intermediate or advanced stage liver cancer.
authors: Department of Laparoscopic Surgery, People's Hospital of Zhengzhou, Zhengzhou 450003, China.
authors: Da-Ming
authors: DM
authors: Zhang
authors: Department of Laparoscopic Surgery, People's Hospital of Zhengzhou, Zhengzhou 450003, China.
authors: Jian-Jun
authors: JJ
authors: Li
authors: Department of Laparoscopic Surgery, People's Hospital of Zhengzhou, Zhengzhou 450003, China.
authors: Peng
authors: P
authors: Yan
authors: Department of Laparoscopic Surgery, People's Hospital of Zhengzhou, Zhengzhou 450003, China. Electronic address: 876893310@qq.com.
authors: Jian-Ting
authors: JT
authors: Hu
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D064593:Heterografts; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D008099:Liver / Q000166:cytology*; D008113:Liver Neoplasms / Q000473:pathology*; D008745:Methylation; D051379:Mice; D008819:Mice, Nude
keywords: Establishment; Fibrocyte; Induced pluripotent stem cells; Liver cancer