title: Intravenous infusion of mesenchymal stem/stromal cells decreased CCR7(+) antigen presenting cells in mice with corneal allotransplantation.
abstract: OBJECTIVE
  To investigate the effects of intravenous (IV) infusion of human mesenchymal stem/stromal cells (hMSCs) on activation and migration of CCR7(+) antigen presenting cells (APCs) in allogeneic corneal transplantation.
  
  
  METHODS
  We first analyzed the cellular and molecular profiles of draining lymph nodes (DLNs) in early and late phases after syngeneic or allogeneic corneal transplantation in mice, and then investigated the effects of hMSCs on APCs expressing CCR7, a key molecule implicated in APC migration to DLNs.
  
  
  RESULTS
  After early transplantation, the numbers of MHC class II(+)CD11b(+)CD11c(-), MHC class II(+)CD11b(-)CD11c(+), and MHC II(+)CD11b(+)CD11c(+) cells as well as the levels of APC-derived cytokines (IL-12a and IL-12b) driving the Th1 response were increased in both syngeneic and allogeneic transplants indicating activation of APCs. In late phase, the numbers of CD3(+)CD4(+)CD8(-) and CD3(+)CD4(-)CD8(+) cells and the levels of T cell-derived cytokines were increased in allogeneic transplants, but not in syngeneic transplants indicating immune rejection. The peri-transplant infusion of IV hMSCs significantly reduced the numbers of CCR7(+)CD11b(+) or CCR7(+)CD11c(+) cells in DLNs and the cornea in the early phase. Also, the expression of CCR7 and its ligands, CCL19, CCL21, and CXC3R as well as IL-12 were markedly decreased by hMSCs in the cornea and DLNs.
  
  
  CONCLUSIONS
  IV hMSCs reduced the activation and migration of CCR7(+) APCs in the cornea and DLNs in allogeneic corneal transplantation.
authors: Department of Ophthalmology, Seoul National University Hospital , Seoul , Korea and.
authors: Hyun Ju
authors: HJ
authors: Lee
authors: Jung Hwa
authors: JH
authors: Ko
authors: Ah Young
authors: AY
authors: Ko
authors: Mee Kum
authors: MK
authors: Kim
authors: Won Ryang
authors: WR
authors: Wee
authors: Joo Youn
authors: JY
authors: Oh
mesh_terms: D064591:Allografts; D000818:Animals; D002465:Cell Movement; D003316:Corneal Diseases / Q000276:immunology / Q000601:surgery; D016039:Corneal Transplantation / Q000379:methods*; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D007262:Infusions, Intravenous; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D054400:Receptors, CCR7 / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
chemical_list: C515371:Ccr7 protein, mouse; D054400:Receptors, CCR7
keywords: Antigen presenting cell; chemokine (C–C motif) receptor 7; cornea; mesenchymal stem/stromal cell; transplantation