title: A simple chemical synthesis of sugar nucleoside diphosphates in water.
abstract: Chemoenzymatic oligosaccharide synthesis is attractive since it eliminates the tedious multistep protection-deprotection requirements of pure chemical synthesis. Chemoenzymatic synthesis using glycosyltransferases, however, requires not only the correct enzyme to control both regio- and stereospecificity, but also the glycosyl donor to provide the sugar that is added. This unit describes a simple synthesis of sugar-nucleoside diphosphates (sugar-NDPs), the type of glycosyl donor (e.g., UDP-Glc, UDP-Gal, ADP-Glc) required by most glycosyltransferases, by using a chemical coupling reaction in water. The preparation of sugar-NDPs by this method therefore does not require any skills in synthetic organic chemistry.
authors: Carlsberg Laboratory, Copenhagen, Denmark.
authors: Hidenori
authors: H
authors: Tanaka
authors: Carlsberg Laboratory, Copenhagen, Denmark.
authors: Yayoi
authors: Y
authors: Yoshimura
authors: Carlsberg Laboratory, Copenhagen, Denmark.
authors: Ole
authors: O
authors: Hindsgaul
mesh_terms: D055162:Biocatalysis; D060326:Chemistry Techniques, Synthetic / Q000379:methods*; D016695:Glycosyltransferases / Q000737:chemistry*; D009702:Nucleoside Diphosphate Sugars / Q000138:chemical synthesis*; D014867:Water / Q000737:chemistry
chemical_list: D009702:Nucleoside Diphosphate Sugars; D014867:Water; D016695:Glycosyltransferases
keywords: chemical synthesis; chemoenzymatic oligosaccharide synthesis; glycosyl-transferase; pyrophosphate formation; sugar nucleotide; sugar-nucleoside diphosphate