title: Complete genome sequence of Agrotis segetum granulovirus Shanghai strain.
abstract: The complete nucleotide sequence of Agrotis segetum granulovirus Shanghai strain (AgseGV-L1) was determined and compared with that of AgseGV Xinjiang strain (AgseGV-XJ). The circular genome of AgseGV-L1 is 131,442 bp and has a G+C content of 37.27 %. It includes 149 ORFs, 24 of which are unique to AgseGV (AgseGV-L1 and AgseGV-XJ [GenBank accession no. NC_005839]). The average level of amino acid sequence identity between AgseGV-L1 and other granulovirus (GV) homologues (except AgseGV-XJ) ranged from 44.3 % (Adoxophyes orana granulovirus [AdorGV]) to 49 % (Cydia pomonella granulovirus [CpGV]). The AgseGV-L1 genome is 99 % identical to that of AgseGV-XJ. They have 196 differences, including 172 substitutions, 21 deletions and 3 insertions. Two homologous regions (hrs) were detected in two intergenic spaces, which share low identity and both lack a palindromic core. A p6.9 gene was found in this genome, which shared 38 %-59 % amino acid identity with those of other baculoviruses. No differences were found in the hr and p6.9 sequences of AgseGV-L1 and AgseGV-XJ. Ie-1 is a known immediate-early gene, but AgseGV-L1 ie-1 has no recognizable promoter element. By BLASTP analysis, one bro gene homologue of NPVs was detected (Agse148). Phylogenetic analysis based on the 29 core baculovirus genes indicated that AgseGV-L1 is closely related to AgseGV-XJ, Helicoverpa armigera granulovirus (HearGV), Helicoverpa armigera granulovirus (XecnGV), Pseudaletia unipuncta granulovirus (PsunGV), Spodoptera litura granulovirus (SpliGV) and Plutella xylostella granulovirus (PlxyGV).
authors: College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, China, lzpzxx@126.com.
authors: Xiaoxia
authors: X
authors: Zhang
authors: Zhenpu
authors: Z
authors: Liang
authors: Xinming
authors: X
authors: Yin
authors: Jianlin
authors: J
authors: Wang
authors: Xinfeng
authors: X
authors: Shao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002681:China; D015967:Gene Expression Regulation, Viral; D016679:Genome, Viral; D025722:Granulovirus / Q000235:genetics* / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology*; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D014764:Viral Proteins