title: Enzymatic synthesis of amylose brushes revisited: details from X-ray photoelectron spectroscopy and spectroscopic ellipsometry.
abstract: The successful synthesis of amylose brushes via enzymatic "grafting from" polymerization and the detailed characterization of all synthetic steps by X-ray photoelectron spectroscopy (XPS) and spectroscopic ellipsometry measurements are reported. Au and Si surfaces are amino-functionalized with self-assembled monolayers (SAMs) of cystamine and 3-aminopropyldimethyethoxysilane (APDMES), respectively. Maltoheptaose is covalently attached to the amino-functionalized Au and Si surfaces via reductive amination. Amylose brushes are grown from maltoheptaose modified Au and Si surfaces with enzymatic polymerization using potato phosphorylase and Rabbit Muscle phosphorylase, as evidenced by spectroscopic ellipsometry and XPS measurements.
authors: Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, NL-9747 AG Groningen, The Netherlands.
authors: Laura
authors: L
authors: Mazzocchetti
authors: Theodoros
authors: T
authors: Tsoufis
authors: Petra
authors: P
authors: Rudolf
authors: Katja
authors: K
authors: Loos
mesh_terms: D000688:Amylose / Q000138:chemical synthesis / Q000737:chemistry*; D060326:Chemistry Techniques, Synthetic; D005936:Glucans / Q000737:chemistry*; D006046:Gold; D006005:Phosphorylases / Q000737:chemistry; D056951:Photoelectron Spectroscopy; D058105:Polymerization; D012825:Silicon; D013057:Spectrum Analysis; D013499:Surface Properties
chemical_list: D005936:Glucans; C031146:maltoheptaose; D006046:Gold; D000688:Amylose; D006005:Phosphorylases; D012825:Silicon
keywords: amylose brushes; ellipsometry; enzymatic polymerization; self-assembled monolayers; x-ray photonelectron spectroscopy