title: Effect of lycopene against cisplatin-induced acute renal injury in rats: organic anion and cation transporters evaluation.
abstract: In the present study, we investigated the effects of lycopene on the expression of organic anion transporters (OATs), organic cation transporters (OCTs), and multidrug resistance-associated proteins (MRPs) of cisplatin-induced nephrotoxicity in rats. Twenty-eight 8-week-old Wistar rats were divided into four groups: control, lycopene-treated (6 mg/kg BW by oral gavage), cisplatin-treated (7 mg/kg BW, IP), and lycopene in combination with cisplatin-treated groups. In the presence of cisplatin, serum urea nitrogen (urea-N) (48.5 vs. 124.3 mg/dl) and creatinine (0.29 vs. 1.37 mg/dl) levels and the kidney efflux transporters MRP2 and MRP4 levels were significantly increased, whereas OAT1, OAT3, OCT1, and OCT2 levels in kidney were decreased in the treated rats compared with normal control rats. However, administration of lycopene in combination with cisplatin resulted in a reduction in the serum urea-N (124.3 vs. 62.4) and creatinine (1.37 vs. 0.40) levels and the kidney efflux transporters MRP2 and MRP4 proteins in the kidneys. Administration of lycopene to acute renal injury-induced rats largely upregulated the organic anion transporters (OAT1 and 3) and organic cation transporters (OCT1 and 2) to decrease the side effects of cisplatin. The present study suggests that lycopene synergizes with its nephroprotective effect against cisplatin-induced acute kidney injury in rats.
authors: Elazig Health High School, Firat University, Elazig, 23119, Turkey.
authors: Fazilet
authors: F
authors: Erman
authors: Mehmet
authors: M
authors: Tuzcu
authors: Cemal
authors: C
authors: Orhan
authors: Nurhan
authors: N
authors: Sahin
authors: Kazim
authors: K
authors: Sahin
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000378:metabolism; D058186:Acute Kidney Injury / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D015153:Blotting, Western; D002338:Carotenoids / Q000494:pharmacology*; D050482:Catecholamine Plasma Membrane Transport Proteins / Q000378:metabolism; D002945:Cisplatin; D000077276:Lycopene; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology; D027362:Organic Anion Transport Protein 1 / Q000378:metabolism; D027361:Organic Anion Transporters / Q000378:metabolism*; D029362:Organic Anion Transporters, Sodium-Independent / Q000378:metabolism; D027701:Organic Cation Transport Proteins / Q000378:metabolism*; D000075102:Organic Cation Transporter 2; D017208:Rats, Wistar; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D018528:ATP-Binding Cassette Transporters; D000975:Antioxidants; D002338:Carotenoids; D050482:Catecholamine Plasma Membrane Transport Proteins; D002945:Cisplatin; D000077276:Lycopene; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D018696:Neuroprotective Agents; D027362:Organic Anion Transport Protein 1; D027361:Organic Anion Transporters; D029362:Organic Anion Transporters, Sodium-Independent; D027701:Organic Cation Transport Proteins; D000075102:Organic Cation Transporter 2; C423013:Abcc2 protein, rat; C489542:Abcc4 protein, rat; C493212:Slc22a1 protein, rat; C100590:Slc22a2 protein, rat; C421043:Slc22a6 protein, rat; C114338:organic anion transport protein 3