title: Comprehensive histological evaluation of bone implants.
abstract: To investigate and assess bone regeneration in sheep in combination with new implant materials classical histological staining methods as well as immunohistochemistry may provide additional information to standard radiographs or computer tomography. Available published data of bone defect regenerations in sheep often present none or sparely labeled histological images. Repeatedly, the exact location of the sample remains unclear, detail enlargements are missing and the labeling of different tissues or cells is absent. The aim of this article is to present an overview of sample preparation, staining methods and their benefits as well as a detailed histological description of bone regeneration in the sheep tibia. General histological staining methods like hematoxylin and eosin, Masson-Goldner trichrome, Movat's pentachrome and alcian blue were used to define new bone formation within a sheep tibia critical size defect containing a polycaprolactone-co-lactide (PCL) scaffold implanted for 3 months (n = 4). Special attention was drawn to describe the bone healing patterns down to cell level. Additionally one histological quantification method and immunohistochemical staining methods are described.
authors: Department of Trauma and Reconstructive Surgery; University Hospital Carl Gustav Carus; Technische Universität Dresden; Dresden, Germany; University Hospital and Medical Faculty; Technische Universität Dresden; Centre for Translational Bone, Joint and Soft Tissue Research; Dresden, Germany.
authors: Claudia
authors: C
authors: Rentsch
authors: Department of Trauma and Reconstructive Surgery; University Hospital Carl Gustav Carus; Technische Universität Dresden; Dresden, Germany.
authors: Wolfgang
authors: W
authors: Schneiders
authors: Department of Trauma and Reconstructive Surgery; University Hospital Carl Gustav Carus; Technische Universität Dresden; Dresden, Germany; University Hospital and Medical Faculty; Technische Universität Dresden; Centre for Translational Bone, Joint and Soft Tissue Research; Dresden, Germany.
authors: Suzanne
authors: S
authors: Manthey
authors: University Hospital and Medical Faculty; Technische Universität Dresden; Centre for Translational Bone, Joint and Soft Tissue Research; Dresden, Germany.
authors: Barbe
authors: B
authors: Rentsch
authors: Department of Trauma and Reconstructive Surgery; University Hospital Carl Gustav Carus; Technische Universität Dresden; Dresden, Germany; University Hospital and Medical Faculty; Technische Universität Dresden; Centre for Translational Bone, Joint and Soft Tissue Research; Dresden, Germany.
authors: Stephan
authors: S
authors: Rammelt
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D018786:Bone Substitutes / Q000737:chemistry*; D005260:Female; D009130:Muscle, Smooth / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry; D019736:Prostheses and Implants; D012756:Sheep; D013194:Staining and Labeling / Q000379:methods; D013977:Tibia / Q000473:pathology / Q000601:surgery; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D000199:Actins; D018786:Bone Substitutes; D011091:Polyesters; C553659:polycaprolactone-co-lactide
keywords: bone implants; histology; polycaprolactone-co-lactide scaffold; sheep