title: An unbalanced PD-L1/CD86 ratio in CD14(++)CD16(+) monocytes is correlated with HCV viremia during chronic HCV infection.
abstract: Circulating monocyte subsets with distinct functions play important roles in hepatitis C virus (HCV) infection. However, the mechanisms have not been well studied. In this study, we analyzed the distributions and phenotypic characteristics of three circulating monocyte subsets-CD14(++)CD16(-), CD14(++)CD16(+) and CD14(+/dim)CD16(+)-in chronic HCV-infected patients, HCV spontaneous resolvers and healthy controls, and we evaluated the possible link between HCV viremia and disease progression. Our results indicated that the frequency of the CD14(++)CD16(+) monocyte subset was decreased, and negatively correlated with HCV RNA and core antigen levels during chronic HCV infection. PD-L1 expression and the PD-L1/CD86 ratio in CD14(++)CD16(+) monocytes were higher during chronic HCV infection than in spontaneous HCV resolvers and healthy controls. The PD-L1/CD86 ratio positively correlated with HCV viral load and core antigen levels. Finally, PD-L1 was significantly increased, while cytokine secretions were dramatically decreased upon Toll-like receptor (TLR) ligand binding and HCV JFH-1stimulation. These findings indicates the compromised immune status of the CD14(++)CD16(+) monocytes during chronic HCV infection and provides new insights into the specific role of the CD14(++)CD16(+) monocytes and their significance in chronic HCV infection.
authors: 1] Department of Microbiology, Peking University Health Science Center, Beijing, China [2] Department of Laboratory Medicine, Peking University Third Hospital, Beijing, China.
authors: Jiajia
authors: J
authors: Zheng
authors: State Key Laboratory for Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, China CDC, Beijing, China.
authors: Hua
authors: H
authors: Liang
authors: Department of Microbiology, Peking University Health Science Center, Beijing, China.
authors: Chunhui
authors: C
authors: Xu
authors: Department of Microbiology, Peking University Health Science Center, Beijing, China.
authors: Qiang
authors: Q
authors: Xu
authors: Department of Microbiology, Peking University Health Science Center, Beijing, China.
authors: Ting
authors: T
authors: Zhang
authors: 1] Department of Microbiology, Peking University Health Science Center, Beijing, China [2] Center of Infectious Diseases, Peking University, Beijing, China.
authors: Tao
authors: T
authors: Shen
authors: 1] Department of Microbiology, Peking University Health Science Center, Beijing, China [2] Center of Infectious Diseases, Peking University, Beijing, China.
authors: Fengmin
authors: F
authors: Lu
mesh_terms: D000328:Adult; D051940:B7-2 Antigen / Q000378:metabolism*; D060890:B7-H1 Antigen / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D016207:Cytokines / Q000378:metabolism; D003710:Demography; D005260:Female; D016174:Hepacivirus / Q000276:immunology*; D019698:Hepatitis C, Chronic / Q000276:immunology* / Q000821:virology; D006801:Humans; D016130:Immunophenotyping; D008024:Ligands; D018950:Lipopolysaccharide Receptors / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D017452:Receptors, IgG / Q000378:metabolism*; D051193:Toll-Like Receptors / Q000378:metabolism; D014766:Viremia / Q000276:immunology*
chemical_list: D051940:B7-2 Antigen; D060890:B7-H1 Antigen; D015415:Biomarkers; C423236:CD274 protein, human; C497964:CD86 protein, human; D016207:Cytokines; D008024:Ligands; D018950:Lipopolysaccharide Receptors; D017452:Receptors, IgG; D051193:Toll-Like Receptors