title: Puerarin protects against lead-induced cytotoxicity in cultured primary rat proximal tubular cells.
abstract: Puerarin, a potent free radicals scavenger, has been demonstrated to have protective efficacy in oxidative damage induced by nephrotoxins. In the present study, the attenuating effect of puerarin (PU) on lead (Pb)-induced apoptosis and oxidative stress was investigated in cultured primary rat proximal tubular (rPT) cells. Results showed that exposure to 0.5 µM Pb induced a decrease in cell viability accompanied with obvious cellular morphological alterations and caused an increase in apoptotic rate and apoptotic morphological changes. Simultaneously, depletion of mitochondrial membrane potential (ΔΨ) and intracellular glutathione (GSH); elevation of caspase-3 activity, intracellular reactive oxygen species, and malondialdehyde levels; and inhibition of GSH peroxidase (GSH-Px) activity were revealed in the cells exposed to Pb alone. However, simultaneous supplementation with PU (50 and 100 µM) protected rPT cells from Pb-induced cytotoxicity through inhibiting apoptosis, attenuating lipid peroxidation, renewing mitochondrial function, and elevating the intracellular antioxidants (nonenzymatic and enzymic) levels. In conclusion, these findings suggested that PU, as a widely distributed dietary antioxidant, contributes potentially to inhibition of Pb-induced cytotoxicity in rPT cells.
authors: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, People's Republic of China.
authors: Gang
authors: G
authors: Liu
authors: Laboratory Animal Center, Shandong University of Traditional Chinese Medicine, Jinan, People's Republic of China.
authors: Zifa
authors: Z
authors: Li
authors: Department of Animal Husbandry and Veterinary Medicine, Beijing Vocational College of Agriculture, Beijing, People's Republic of China.
authors: Jinqiu
authors: J
authors: Wang
authors: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, People's Republic of China.
authors: Hong
authors: H
authors: Wang
authors: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, People's Republic of China.
authors: Zhenyong
authors: Z
authors: Wang
authors: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, People's Republic of China wanglin2013@sdau.edu.cn.
authors: Lin
authors: L
authors: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D019610:Cytoprotection; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007529:Isoflavones / Q000494:pharmacology*; D007687:Kidney Tubules, Proximal / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007854:Lead / Q000633:toxicity*; D008315:Malondialdehyde / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D009566:Nitrates / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D061251:Primary Cell Culture; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D016166:Free Radical Scavengers; D007529:Isoflavones; D009566:Nitrates; D017382:Reactive Oxygen Species; D007854:Lead; D008315:Malondialdehyde; C017461:lead nitrate; D005979:Glutathione Peroxidase; C505410:Casp3 protein, rat; D053148:Caspase 3; D005978:Glutathione; C033607:puerarin
keywords: Lead; apoptosis; oxidative stress; proximal tubular cells; puerarin