title: Pinpointing double bonds in lipids by Paternò-Büchi reactions and mass spectrometry.
abstract: The positions of double bonds in lipids play critical roles in their biochemical and biophysical properties. In this study, by coupling Paternò-Büchi (P-B) reaction with tandem mass spectrometry, we developed a novel method that can achieve confident, fast, and sensitive determination of double bond locations within various types of lipids. The P-B reaction is facilitated by UV irradiation of a nanoelectrospray plume entraining lipids and acetone. Tandem mass spectrometry of the on-line reaction products via collision activation leads to the rupture of oxetane rings and the formation of diagnostic ions specific to the double bond location.
authors: Department of Chemistry, Purdue University, 560 Oval Dr., West Lafayette, IN (USA) http://www.chem.purdue.edu/xia/
authors: Xiaoxiao
authors: X
authors: Ma
authors: Yu
authors: Y
authors: Xia
mesh_terms: D000096:Acetone / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D015394:Molecular Structure; D053719:Tandem Mass Spectrometry; D014466:Ultraviolet Rays
chemical_list: D008055:Lipids; D000096:Acetone
keywords: Paternò-Büchi reaction; double bond location; photochemical reactions; tandem mass spectrometry; unsaturated lipids