title: Dynamic Wnt5a expression in murine hair follicle cycle and its inhibitory effects on follicular.
abstract: OBJECTIVE
  To analyze the dynamic expression of Wnt family member 5A (Wingless-type MMTV integration Wnt site family, member 5a) in murine hair cycle and its inhibitory effects on follicle in vivo.
  
  
  METHODS
  Situ hybridization in full-thickness skin was used to observe the change of mouse protein expression in different growth stages, and Ad-Wnt5a was injected after defeathering to observe the hair follicle growth in vivo.
  
  
  RESULTS
  The Wnt5a mRNA was expressed at birth, and was firstly increased then decreased along with the progress of the hair cycle. It reached the peak in advanced stage of growth cycle (P<0.05). Rhoa and β-catenin expression levels were significantly decreased in three groups. Rac2 expression was significantly up-regulated, and the expression level of Wnt5a, Shh and Frizzled2 was increased, but less significantly than group 2.
  
  
  CONCLUSIONS
  The expression of Wnt5a mRNA is consistent with change of murine follicle cycle, and has obvious inhibitory effects on the growth of hair follicle in vivo, indicating that it is antagonistic to Wnts pathway and interferes the growth of follicle together.
authors: Department of Dermatology, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China. Electronic address: fangdr@gmail.com.
authors: De-Ren
authors: DR
authors: Fang
authors: Department of Dermatology, the Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China.
authors: Zhong-Fa
authors: ZF
authors: Lv
authors: Department of Dermatology, the Third People's Hospital of Hanghzhou, Hanghzhou, 310009 China.
authors: Gang
authors: G
authors: Qiao
mesh_terms: D000818:Animals; D016009:Chi-Square Distribution; D020869:Gene Expression Profiling; D018859:Hair Follicle / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012867:Skin / Q000378:metabolism; D051153:Wnt Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D000071818:Wnt-5a Protein
chemical_list: D012333:RNA, Messenger; D051153:Wnt Proteins; D000071818:Wnt-5a Protein; C438615:Wnt5a protein, mouse
keywords: Hair follicle cycle; Iinhibition; Proliferation; Wnt5a