title: Canibacter oris gen. nov., sp. nov., isolated from an infected human wound.
abstract: A facultatively anaerobic, Gram-reaction-positive, catalase- and oxidase-negative, rod-shaped bacterium isolated from an infected human wound caused by a dog bite was characterized by phenotypic and molecular genetic methods. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain IMMIB Q2029717T was a member of the order Micrococcales of the class Actinobacteria, displaying 91.6% to 96% sequence similarity with members of the family Microbacteriaceae. Phylogentic trees generated by different algorithms indicated that the strain forms an independent phylogenetic line of descent that consistently clustered proximal to the base of the genus Leucobacter. Chemical studies revealed the presence of a cell-wall murein based on L-lysine (type B1α), major menaquinone (MK-10) and a DNA G+C content of 56.9 mol%. The distinct phylogenetic position, ribotyping and matrix-assisted laser desorption/ionization time-of-flight MS profiles and the significant phenotypic differences clearly separate strain IMMIB Q2029717T from its nearest phylogenetic neighbour and support its classification as a representative of a novel genus and species, with the suggested name Canibacter oris gen. nov., sp. nov. The type strain is IMMIB Q2029717T (=DSM 27064T=CCUG 64069T).
authors: Bacteriology Laboratory, Department of Microbiology and Infectious Diseases, Laboratory Medicine of Western Australia, QEII Medical Centre, Nedlands WA 6009, Australia.
authors: M
authors: M
authors: Aravena-Román
authors: Bacteriology Laboratory, Department of Microbiology and Infectious Diseases, Laboratory Medicine of Western Australia, QEII Medical Centre, Nedlands WA 6009, Australia.
authors: T J J
authors: TJJ
authors: Inglis
authors: Institut für Organische Chemie, Johannes Gutenberg-Universität Mainz, Duesbergweg 10-14 55128 Mainz, Germany.
authors: C
authors: C
authors: Siering
authors: Leibniz Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstrasse 7b, D-38124 Braunschweig, Germany.
authors: P
authors: P
authors: Schumann
authors: Institut für Medizinische Mikrobiologie und Immunologie der Universität Bonn, Sigmund-Freud-Straße 25, 53127 Bonn, Germany.
authors: A F
authors: AF
authors: Yassin
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001733:Bites and Stings / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D004285:Dogs; D005260:Female; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D021521:Ribotyping; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2