title: Isolation of single-base genome-edited human iPS cells without antibiotic selection.
abstract: Precise editing of human genomes in pluripotent stem cells by homology-driven repair of targeted nuclease-induced cleavage has been hindered by the difficulty of isolating rare clones. We developed an efficient method to capture rare mutational events, enabling isolation of mutant lines with single-base substitutions without antibiotic selection. This method facilitates efficient induction or reversion of mutations associated with human disease in isogenic human induced pluripotent stem cells.
authors: Gladstone Institute of Cardiovascular Disease, San Francisco, California, USA.
authors: Yuichiro
authors: Y
authors: Miyaoka
authors: Gladstone Institute of Cardiovascular Disease, San Francisco, California, USA.
authors: Amanda H
authors: AH
authors: Chan
authors: 1] Gladstone Institute of Cardiovascular Disease, San Francisco, California, USA. [2] Department of Pediatrics, University of California San Francisco, San Francisco, California, USA.
authors: Luke M
authors: LM
authors: Judge
authors: Gladstone Institute of Cardiovascular Disease, San Francisco, California, USA.
authors: Jennie
authors: J
authors: Yoo
authors: Department of Neurology, University of California San Francisco, San Francisco, California, USA.
authors: Miller
authors: M
authors: Huang
authors: Gladstone Institute of Cardiovascular Disease, San Francisco, California, USA.
authors: Trieu D
authors: TD
authors: Nguyen
authors: Gladstone Institute of Cardiovascular Disease, San Francisco, California, USA.
authors: Paweena P
authors: PP
authors: Lizarraga
authors: Gladstone Institute of Cardiovascular Disease, San Francisco, California, USA.
authors: Po-Lin
authors: PL
authors: So
authors: 1] Gladstone Institute of Cardiovascular Disease, San Francisco, California, USA. [2] Department of Medicine, University of California San Francisco, San Francisco, California, USA. [3] Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California, USA.
authors: Bruce R
authors: BR
authors: Conklin
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001482:Base Composition / Q000235:genetics; D002460:Cell Line; D003001:Cloning, Molecular; D003584:Cytological Techniques / Q000379:methods*; D015894:Genome, Human; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects; D009154:Mutation
chemical_list: D000900:Anti-Bacterial Agents