title: Determination of amitraz and its metabolites in whole blood using solid-phase extraction and liquid chromatography-tandem mass spectrometry.
abstract: A method was developed for determination of amitraz and its metabolites, N-[2,4-(dimethylphenyl)-N'-methylformamidine (DMPF), 2,4-dimethylformamidine (DMF), 2,4-dimethylaniline (DMA) in whole blood. The analytes were extracted by solid-phase extraction (SPE) using dichloromethane, acetonitrile and methanol (2:1:1) mixture as elute solution. Analysis was performed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in the positive ion mode using multiple reaction monitoring (MRM) technique. Collision-induced dissociation (CID) of amitraz at the electrospray source in MS/MS was observed in the analytic conditions. The method was validated in human whole blood spiked at three concentration levels. The low limit of detection (LOD) and the low limit of quantification (LOQ) for all the analytes were below 0.5μg/L and 2μg/L, respectively. Recoveries were between 90.2% and 104.5%, Bias and relative standard deviation (RSD) were below 15% (n=6). The good linear relationships were obtained in certain concentration ranges of amitraz and its metabolites. The results demonstrated the method is exclusive, sensitive and accurate, and can be applied in forensic toxicology.
authors: Center of Evidence Identification, Chongqing Public Security Bureau, Chongqing 400021, China.
authors: Hao
authors: H
authors: Guo
authors: Center of Evidence Identification, Chongqing Public Security Bureau, Chongqing 400021, China. Electronic address: panzhang.chem@gmail.com.
authors: Pan
authors: P
authors: Zhang
authors: Center of Evidence Identification, Chongqing Public Security Bureau, Chongqing 400021, China.
authors: Junwei
authors: J
authors: Wang
authors: Center of Evidence Identification, Chongqing Public Security Bureau, Chongqing 400021, China.
authors: Jing
authors: J
authors: Zheng
mesh_terms: D002675:Child, Preschool; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014052:Toluidines / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification / Q000506:poisoning
chemical_list: D014052:Toluidines; C014983:amitraz
keywords: Amitraz; LC–MS/MS; SPE; Whole blood