title: Comparison and investigation of bovine hemoglobin binding to dihydroartemisinin and 9-hydroxy-dihydroartemisinin: spectroscopic characterization.
abstract: The UV-vis absorption, steady state/time resolved fluorescence spectroscopy and synchronous fluorescence, circular dichroism (CD) spectroscopy are used to investigate the interaction mechanisms of dihydroartemisinin (DHA) and 9-hydroxy-dihydroartemisinin (9-OH DHA), respectively. The UV-vis studies present that DHA and 9-OH DHA can disturb the structure of bovine hemoglobin (BHb). Steady state/time resolved and synchronous fluorescence spectroscopy reveal that the binding constant of DHA with BHb is bigger than 9-OH DHA. CD spectra indicate DHA and 9-OH DHA can change the conformation of BHb. The comparison results suggest that the binding of BHb with DHA is more stable and stronger than 9-OH DHA.
authors: Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Materials Science, Analysis and Testing Center, Nanjing Normal University, Nanjing 210023, China; Jiangsu Key Laboratory of Biomedical Materials, College of Chemistry and Materials Science, Analysis and Testing Center, Key Laboratory of Applied Photochemistry, Nanjing Normal University, Nanjing 210023, China.
authors: Mengsi
authors: M
authors: Xiao
authors: Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Materials Science, Analysis and Testing Center, Nanjing Normal University, Nanjing 210023, China; Jiangsu Key Laboratory of Biomedical Materials, College of Chemistry and Materials Science, Analysis and Testing Center, Key Laboratory of Applied Photochemistry, Nanjing Normal University, Nanjing 210023, China.
authors: Lina
authors: L
authors: Han
authors: Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Materials Science, Analysis and Testing Center, Nanjing Normal University, Nanjing 210023, China; Jiangsu Key Laboratory of Biomedical Materials, College of Chemistry and Materials Science, Analysis and Testing Center, Key Laboratory of Applied Photochemistry, Nanjing Normal University, Nanjing 210023, China.
authors: Lin
authors: L
authors: Zhou
authors: Department of Physical Science and Technology, Nanjing Normal University, Nanjing 210023, China.
authors: Yanhuai
authors: Y
authors: Zhou
authors: Department of Physical Science and Technology, Nanjing Normal University, Nanjing 210023, China.
authors: Xiaoqin
authors: X
authors: Huang
authors: Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Materials Science, Analysis and Testing Center, Nanjing Normal University, Nanjing 210023, China; Jiangsu Key Laboratory of Biomedical Materials, College of Chemistry and Materials Science, Analysis and Testing Center, Key Laboratory of Applied Photochemistry, Nanjing Normal University, Nanjing 210023, China.
authors: Xuefeng
authors: X
authors: Ge
authors: Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Materials Science, Analysis and Testing Center, Nanjing Normal University, Nanjing 210023, China; Jiangsu Key Laboratory of Biomedical Materials, College of Chemistry and Materials Science, Analysis and Testing Center, Key Laboratory of Applied Photochemistry, Nanjing Normal University, Nanjing 210023, China.
authors: Shaohua
authors: S
authors: Wei
authors: Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Materials Science, Analysis and Testing Center, Nanjing Normal University, Nanjing 210023, China; Jiangsu Key Laboratory of Biomedical Materials, College of Chemistry and Materials Science, Analysis and Testing Center, Key Laboratory of Applied Photochemistry, Nanjing Normal University, Nanjing 210023, China. Electronic address: zhoujiahong@njnu.edu.cn.
authors: Jiahong
authors: J
authors: Zhou
authors: Institute of Stomatology, Department of Oral and Maxillofacial Surgery, Nanjing Medical University, No. 136, Hanzhong Avenue, Nanjing, China. Electronic address: whmz2002@yahoo.cn.
authors: Heming
authors: H
authors: Wu
authors: Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Materials Science, Analysis and Testing Center, Nanjing Normal University, Nanjing 210023, China; Jiangsu Key Laboratory of Biomedical Materials, College of Chemistry and Materials Science, Analysis and Testing Center, Key Laboratory of Applied Photochemistry, Nanjing Normal University, Nanjing 210023, China.
authors: Jian
authors: J
authors: Shen
mesh_terms: D000818:Animals; D037621:Artemisinins / Q000737:chemistry* / Q000378:metabolism*; D002417:Cattle; D002942:Circular Dichroism; D006454:Hemoglobins / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D011485:Protein Binding; D017433:Protein Structure, Secondary; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics; D013997:Time Factors
chemical_list: C000593166:9-hydroxy-dihydroartemisinin; D037621:Artemisinins; D006454:Hemoglobins; D012996:Solutions; C039060:artenimol
keywords: 9-OH DHA; Bovine hemoglobin; Comparison; DHA; Spectroscopic characterization