title: Potential of a white-rot fungus Pleurotus eryngii F032 for degradation and transformation of fluorene.
abstract: The white-rot fungus Pleurotus eryngii F032 showed the capability to degrade a three fused-ring aromatic hydrocarbons fluorene. The elimination of fluorene through sorption was also investigated. Enzyme production is accompanied by an increase in biomass of P. eryngii F032 during degradation process. The fungus totally degraded fluorine within 23 d at 10-mg l(-1) solution. Fluorene degradation was affected with initial fluorene concentrations. The highest enzyme activity was shown by laccase in the 10-mg l(-1) culture after 30 d of incubation (1620 U l(-1)). Few activities of enzymes were observed in the fungal cell at the varying concentration of fluorene. Three metabolic were detected and separated in ethylacetate extract, after isolated by column chromatography. The metabolites, 9-fluorenone, phthalic acid, and benzoic acid were identified using UV-vis spectrophotometer and gas chromatography-mass spectrometry (GC-MS). The results show the presence of a complex mechanism for the regulation of fluorene-degrading enzymes.
authors: Institute of Environmental and Water Resources Management, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Malaysia. Electronic address: hadibarata@utm.my.
authors: Tony
authors: T
authors: Hadibarata
authors: Division of Sustainable Energy and Environmental Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 567-0871, Japan.
authors: Risky Ayu
authors: RA
authors: Kristanti
mesh_terms: D001711:Biotransformation; D004798:Enzymes / Q000032:analysis; D005449:Fluorenes / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D053858:Metabolic Networks and Pathways; D020076:Pleurotus / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D004798:Enzymes; D005449:Fluorenes; C041509:fluorene
keywords: 9-Fluorenone; Benzoic acid; Fluorene biotransformation; Ligninolytic enzymes; Metabolic pathway