title: The influence of supramolecular structure of cellulose allomorphs on the interactions with cellulose-binding domain, CBD3b from Paenibacillus barcinonensis.
abstract: The interaction of recombinant cellulose-binding domains (CBDs) of endoglucanase Cel9B from Paenibacillus barcinonensis with different cotton cellulose allomorphs (I, II and III) has been investigated, in order to bring new insights regarding the CBD adsorption and desorption processes. The highest CBD adsorption capacity was recorded for cellulose I, confirming the affinity of proteins to the most crystalline substrate. The weakening and splitting of the hydrogen bonds within cellulose structure after CBD adsorption, as well as a decrease of the crystallinity degree were identified by ATR-FTIR spectroscopy and XRD. The CBD's adsorption kinetic was shown to be rendered by properties as, specific surface area and porosity, being confirmed by dynamic vapor sorption measurements. An important influence of temperature (25, 37 and 50°C) and/or pH medium (4, 5.5, 7 and 10) on the CBD desorption capacity was confirmed, being related to the hydrophobic interactions formed between the CBD and the cellulose allomorphs.
authors: "Petru Poni" Institute of Macromolecular Chemistry, 41A Grigore Ghica Voda Alley, 700487 Iasi, Romania.
authors: Diana
authors: D
authors: Ciolacu
authors: Department of Microbiology, Faculty of Biology, University of Barcelona, Av. Diagonal 643, 08028 Barcelona, Spain.
authors: Alina Iulia
authors: AI
authors: Chiriac
authors: Department of Microbiology, Faculty of Biology, University of Barcelona, Av. Diagonal 643, 08028 Barcelona, Spain.
authors: F I Javier
authors: FI
authors: Pastor
authors: University of Maribor, Institute of Engineering Materials and Design, Smetanova ulica 17, 2000 Maribor, Slovenia. Electronic address: vanja.kokol@um.si.
authors: Vanja
authors: V
authors: Kokol
mesh_terms: D000327:Adsorption; D000595:Amino Acid Sequence; D002480:Cellulase / Q000737:chemistry* / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry* / Q000378:metabolism*; D003460:Crystallization; D006860:Hydrogen Bonding; D008969:Molecular Sequence Data; D056507:Paenibacillus / Q000201:enzymology*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012044:Regression Analysis; D017550:Spectroscopy, Fourier Transform Infrared; D013227:Steam; D013696:Temperature; D014961:X-Ray Diffraction
chemical_list: D013227:Steam; D002482:Cellulose; D002480:Cellulase
keywords: Adsorption; Cellulose allomorphs; Cellulose binding domain; Desorption; Endoglucanase