title: Intraocular concentration of moxifloxacin after intracameral injection combined with presoaked intraocular lenses.
abstract: OBJECTIVE
  To evaluate the impact of intraocular lens (IOL) moxifloxacin presoaking time on the intraocular concentration of moxifloxacin achieved after intracameral moxifloxacin injection.
  
  
  METHODS
  Harlan Biotech Israel, Rehovot, Israel.
  
  
  METHODS
  Laboratory study
  
  
  METHODS
  Sixty eyes of 30 rabbits were divided into 2 groups after crystalline lens evacuation. In Group A (30 eyes), hydrophilic acrylic IOLs presoaked for 15 minutes in 5 mg/mL moxifloxacin were implanted. In Group B (30 eyes), the same hydrophilic acrylic IOLs presoaked in the same solution for 24 hours were implanted. Intracameral injection of 100 mcg/0.1 moxifloxacin was performed at the end of surgery in both groups. Aqueous humor samples were obtained 2, 4, 6, 8, and 10 hours after IOL implantation and were analyzed by high-performance liquid chromatography to determine the antibiotic concentration.
  
  
  RESULTS
  Group A achieved mean postoperative moxifloxacin concentrations of 18.60 mcg/mL±8.80 (SD), 4.08±6.03 mcg/mL, 1.50±0.75 mcg/mL, 0.21±0.09 mcg/mL, and 0.12±0.04 mcg/mL at 2, 4, 6, 8, and 10 hours, respectively. Group B achieved mean moxifloxacin concentrations of 17.25±6.27 mcg/mL, 9.46±2.22 mcg/mL, 6.26±1.22 mcg/mL, 4.34±0.42 mcg/mL, and 3.62±1.02 mcg/mL, respectively. Moxifloxacin concentrations at 6, 8, and 10 hours were statistically significantly higher in Group B than in Group A (all P<.0001).
  
  
  CONCLUSIONS
  Combining intracameral moxifloxacin injection with implantation of moxifloxacin-presoaked hydrophilic acrylic IOLs yielded high intraocular concentrations of moxifloxacin. Higher concentrations were found with longer presoaking.
  
  
  BACKGROUND
  No author has a financial or proprietary interest in any material or method mentioned.
authors: From the School of Medicine (Lipnitzki), Hebrew University and Hadassah, Jerusalem, Harlan Biotech Israel Ltd. (Ben Eliahu, Ezov), and the Ophthalmology Department (Marcovitz, Kleinmann), Kaplan Medical Center, Rehovot, Israel.
authors: Inna
authors: I
authors: Lipnitzki
authors: From the School of Medicine (Lipnitzki), Hebrew University and Hadassah, Jerusalem, Harlan Biotech Israel Ltd. (Ben Eliahu, Ezov), and the Ophthalmology Department (Marcovitz, Kleinmann), Kaplan Medical Center, Rehovot, Israel.
authors: Shmuel
authors: S
authors: Ben Eliahu
authors: From the School of Medicine (Lipnitzki), Hebrew University and Hadassah, Jerusalem, Harlan Biotech Israel Ltd. (Ben Eliahu, Ezov), and the Ophthalmology Department (Marcovitz, Kleinmann), Kaplan Medical Center, Rehovot, Israel.
authors: Arie L
authors: AL
authors: Marcovitz
authors: From the School of Medicine (Lipnitzki), Hebrew University and Hadassah, Jerusalem, Harlan Biotech Israel Ltd. (Ben Eliahu, Ezov), and the Ophthalmology Department (Marcovitz, Kleinmann), Kaplan Medical Center, Rehovot, Israel.
authors: Natan
authors: N
authors: Ezov
authors: From the School of Medicine (Lipnitzki), Hebrew University and Hadassah, Jerusalem, Harlan Biotech Israel Ltd. (Ben Eliahu, Ezov), and the Ophthalmology Department (Marcovitz, Kleinmann), Kaplan Medical Center, Rehovot, Israel. Electronic address: guy.kleinmann@hsc.utah.edu.
authors: Guy
authors: G
authors: Kleinmann
mesh_terms: D000180:Acrylic Resins; D000818:Animals; D000867:Anterior Chamber / Q000187:drug effects; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*; D001082:Aqueous Humor / Q000378:metabolism*; D001372:Aza Compounds / Q000008:administration & dosage / Q000493:pharmacokinetics*; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid; D004337:Drug Carriers; D024841:Fluoroquinolones; D056965:Injections, Intraocular; D019654:Lens Implantation, Intraocular; D007910:Lenses, Intraocular; D000077266:Moxifloxacin; D018918:Phacoemulsification; D011804:Quinolines / Q000008:administration & dosage / Q000493:pharmacokinetics*; D011817:Rabbits; D013997:Time Factors
chemical_list: D000180:Acrylic Resins; D000900:Anti-Bacterial Agents; D001372:Aza Compounds; D004337:Drug Carriers; D024841:Fluoroquinolones; D011804:Quinolines; D000077266:Moxifloxacin