title: Human platelet microRNA-mRNA networks associated with age and gender revealed by integrated plateletomics.
abstract: There is little data considering relationships among human RNA, demographic variables, and primary human cell physiology. The platelet RNA and expression-1 study measured platelet aggregation to arachidonic acid, ADP, protease-activated receptor (PAR) 1 activation peptide (PAR1-AP), and PAR4-AP, as well as mRNA and microRNA (miRNA) levels in platelets from 84 white and 70 black healthy subjects. A total of 5911 uniquely mapped mRNAs and 181 miRNAs were commonly expressed and validated in a separate cohort. One hundred twenty-nine mRNAs and 15 miRNAs were differentially expressed (DE) by age, and targets of these miRNAs were over-represented among these mRNAs. Fifty-four mRNAs and 9 miRNAs were DE by gender. Networks of miRNAs targeting mRNAs, both DE by age and gender, were identified. The inverse relationship in these RNA pairs suggests miRNAs regulate mRNA levels on aging and between genders. A simple, interactive public web tool (www.plateletomics.com) was developed that permits queries of RNA levels and associations among RNA, platelet aggregation and demographic variables. Access to these data will facilitate discovery of mechanisms of miRNA regulation of gene expression. These results provide new insights into aging and gender, and future platelet RNA association studies must account for age and gender.
authors: Department of Structural and Computational Biology and Molecular Biophysics, and.
authors: Lukas M
authors: LM
authors: Simon
authors: Leonard C
authors: LC
authors: Edelstein
authors: Srikanth
authors: S
authors: Nagalla
authors: Angela B
authors: AB
authors: Woodley
authors: Edward S
authors: ES
authors: Chen
authors: Xianguo
authors: X
authors: Kong
authors: Lin
authors: L
authors: Ma
authors: Paolo
authors: P
authors: Fortina
authors: Satya
authors: S
authors: Kunapuli
authors: Michael
authors: M
authors: Holinstat
authors: Steven E
authors: SE
authors: McKenzie
authors: Jing-Fei
authors: JF
authors: Dong
authors: Chad A
authors: CA
authors: Shaw
authors: Paul F
authors: PF
authors: Bray
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D001792:Blood Platelets / Q000378:metabolism*; D005260:Female; D053263:Gene Regulatory Networks; D023281:Genomics / Q000379:methods; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012737:Sex Factors; D055815:Young Adult
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger