title: Protease characteristics of bacteriocin producing Lysinibacilli, isolated from fruits and vegetable waste.
abstract: This study describes the physical stability and optimization of nutrient components for an extracellular protease produced by Bacillus strains isolated from fruits and vegetable waste, Lucknow, India. The isolated proteases could hydrolyze various native proteinaceous substrates such as bovine serum albumin, casein, skim milk, but not the gelatin. The strain JX416854 and isolate 10 yielded maximum protease (831; 703 U/ml) under optimized conditions: Nutrient, Casein broth; pH 7.0; shaking condition 37°C for 36 h. Crude protease exhibited activity over a wide range of pH (6.0-10.0) and found to be stable at (10-70°C), pH stable at 7- 9.0. The significant protease activity was observed with divalent cations Ca2+ and Mg2+ and EDTA. Further, significant blood destaining properties and stabilities with detergents were also observed. Thus, the significant potency and stability of these enzymes indicated their industrial importance and could be an alternative protease for various industrial applications.
authors: Department of Biosciences, Integral University, Kursi Road, Dasauli, Lucknow- 226026, India.
authors: Varish
authors: V
authors: Ahmad
authors: Department of Biosciences, Integral University, Kursi Road, Dasauli, Lucknow- 226026, India.
authors: Azhar
authors: A
authors: Kamal
authors: Department of Biosciences, Integral University, Kursi Road, Dasauli, Lucknow- 226026, India.
authors: Khurshid
authors: K
authors: Ahmad
authors: Department of Biosciences, Integral University, Kursi Road, Dasauli, Lucknow- 226026, India.
authors: Mohd Sajid
authors: MS
authors: Khan
keywords: 16S r RNA gene; Lysinibacillus; Phylogenetic analysis; Protease