title: Identification of malic enzyme mutants depending on 1,2,3-triazole moiety-containing nicotinamide adenine dinucleotide analogs.
abstract: An activity screening between 1,2,3-triazole moiety-containing nicotinamide adenine dinucleotide (NAD) analogs and malic enzyme (ME) mutants identified some mutants capable of taking NAD analogs as the cofactor. One particular pair, ME-L310K/L404S and the analog B-8 had good catalytic efficiency and cofactor specificity. The new system gained about 1200-fold cofactor specificity shift from NAD toward B-8 in terms of oxidative decarboxylation of l-malate. Our results provided insightful information for the development of orthogonal redox system that is of particular important to precisely control engineered metabolic pathways.
authors: Division of Biotechnology and State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, CAS, Dalian 116023, China; Department of Chemistry, Liaoning Key Laboratory for the Synthesis and Application of Functional Compounds, Bohai University, Jinzhou 121013, China.
authors: Shuhua
authors: S
authors: Hou
authors: Division of Biotechnology and State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, CAS, Dalian 116023, China.
authors: Debin
authors: D
authors: Ji
authors: Division of Biotechnology and State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, CAS, Dalian 116023, China.
authors: Wujun
authors: W
authors: Liu
authors: Division of Biotechnology and State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, CAS, Dalian 116023, China.
authors: Lei
authors: L
authors: Wang
authors: Division of Biotechnology and State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, CAS, Dalian 116023, China. Electronic address: zhaozb@dicp.ac.cn.
authors: Zongbao Kent
authors: ZK
authors: Zhao
mesh_terms: D055162:Biocatalysis; D003653:Decarboxylation; D007700:Kinetics; D008291:Malate Dehydrogenase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008293:Malates / Q000737:chemistry / Q000378:metabolism; D009154:Mutation; D009243:NAD / Q000031:analogs & derivatives* / Q000378:metabolism; D010084:Oxidation-Reduction; D011485:Protein Binding; D014230:Triazoles / Q000737:chemistry*
chemical_list: D008293:Malates; D014230:Triazoles; D009243:NAD; C030298:malic acid; D008291:Malate Dehydrogenase; C030574:malate dehydrogenase (decarboxylating)
keywords: Cofactor; Enzyme kinetics; Malic enzyme; Nicotinamide adenine dinucleotide; Orthogonal redox system