title: The prognostic significance of eukaryotic elongation factor 1 alpha-2 in non-small cell lung cancer.
abstract: BACKGROUND
  Eukaryotic elongation factor 1 alpha-2 (eEF1A2) has been recently shown to be a putative oncogene of lung cancer.
  
  
  METHODS
  We analyzed the expression and prognostic significance of eEF1A2 in 69 primary non-small cell lung cancer (NSCLC) cases. We also suppressed eEF1A2 expression using RNA interference and then analyzed cell proliferation, migration and invasion of five adenocarcinoma cell lines.
  
  
  RESULTS
  eEF1A2 protein expression was positive in 84.1%. Negative immunostaining for eEF1A2 was shown to be an independent prognostic factor and significantly correlated with lymph node metastasis. There was no significant correlation between eEF1A2 protein and mRNA expression levels. Among the five examined cell lines, transfection of eEF1A2 siRNA inhibited cell migration in only one cell line while it did not change cell proliferation and invasion.
  
  
  CONCLUSIONS
  Negative immunostaining of eEF1A2 predicted for poor prognosis of NSCLC. The mechanism of this result could not be elucidated by cell proliferation, migration and invasion assays.
authors: Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, Seiryo-machi 4-1, Aoba-ku, Sendai, Miyagi 980-8575, Japan. m.kawamura@idac.tohoku.ac.jp.
authors: Masaki
authors: M
authors: Kawamura
authors: Chiaki
authors: C
authors: Endo
authors: Akira
authors: A
authors: Sakurada
authors: Fumihiko
authors: F
authors: Hoshi
authors: Hirotsugu
authors: H
authors: Notsuda
authors: Takashi
authors: T
authors: Kondo
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D048708:Cell Growth Processes / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D020648:Peptide Elongation Factor 1 / Q000096:biosynthesis* / Q000235:genetics; D011379:Prognosis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
chemical_list: C503234:EEF1A2 protein, human; D020648:Peptide Elongation Factor 1; D012333:RNA, Messenger
keywords: NSCLC; eEF1A2; immunohistochemistry; lymphnode metastasis; survival analysis