title: Diagnosis of Helicobacter pylori infection by invasive and noninvasive tests.
abstract: Although several invasive and noninvasive tests have been developed for the diagnosis of Helicobacter pylori infection, all of the tests have their limitations. We conducted a study to investigate and compare the suitability of rapid urease test (RUT), serology, histopathology and stool antigen tests with polymerase chain reaction (PCR) for detection of H. pylori, and correlate the diagnostic methods with PCR. Eighty nine patients (61 adults, 28 children) referred to the Firoozgar Hospital and Children Medical Center Hospital for diagnostic upper gastrointestinal endoscopy entered to the study and noninvasive tests such as immunoassay for serological antibodies against H. pylori and detection of its antigen in feces were measured. The biopsies were utilized for histological examination, RUT and PCR. The H. pylori statuses were evaluated by the positivity of ureC PCR in biopsy specimens and 53 subjects had H. pylori positive result. Histopathology showed high overall performance in adults and children with sensitivity and specificity 100% and 90%, respectively. Sensitivity, specificity, and accuracy for stool antigen test were 87.8%, 75% and 82%, respectively. Correlation of RUT, serology (IgG), histopathology and stool antigen tests with PCR were 0.82, 0.32, 0.91 and 0.63, respectively. In conclusion, the RUT and histopathology are as accurate as the PCR of biopsy and stool antigen test can consider as appropriate noninvasive test for detection of H. pylori infection.
authors: Pediatric Infectious Diseases Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors: Babak
authors: B
authors: Pourakbari
authors: Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors: Mona
authors: M
authors: Ghazi
authors: Pediatric Infectious Diseases Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors: Shima
authors: S
authors: Mahmoudi
authors: Pediatric Infectious Diseases Research Center, Tehran University of Medical Sciences, Tehran, Iran. ; Department of Pediatric Infectious Diseases, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors: Setareh
authors: S
authors: Mamishi
authors: Department of Internal Medicine, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors: Hossein
authors: H
authors: Azhdarkosh
authors: Department of Pediatric Gastroenterology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors: Mehri
authors: M
authors: Najafi
authors: Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors: Bahram
authors: B
authors: Kazemi
authors: Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors: Ali
authors: A
authors: Salavati
authors: Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors: Akbar
authors: A
authors: Mirsalehian
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000942:Antigens, Bacterial / Q000032:analysis; D001706:Biopsy; D001774:Blood Chemical Analysis; D002648:Child; D019411:Clinical Laboratory Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D003955:Diagnostic Tests, Routine / Q000379:methods*; D005243:Feces / Q000737:chemistry; D005753:Gastric Mucosa / Q000382:microbiology; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014510:Urease / Q000032:analysis; D055815:Young Adult
chemical_list: D000942:Antigens, Bacterial; D004269:DNA, Bacterial; D014510:Urease
keywords: Helicobacter pylori; diagnosis; invasive and noninvasive tests