title: [Effect of ginsenoside Rb1 on cerebral infarction volume and IL-1 beta in the brain tissue and sera of focal cerebral ischemia/reperfusion injury model rats].
abstract: OBJECTIVE
  To investigate the effect of ginsenoside Rb1 on cerebral infarction volume as well as IL-1 beta in the brain tissue and sera of focal cerebral ischemia/reperfusion (I/R) injury model rats.
  
  
  METHODS
  The I/R rat model was established by using thread according to Zea-Longa. SD rats were randomly divided into five groups, i.e., the sham-operation group, the model group, the low dose ginsenoside Rb1 (20 mg/kg) group, the medium dose ginsenoside Rb1 group (40 mg/kg), and the high dose ginsenoside Rb1 group (80 mg/kg), 12 in each group. Rats in the sham-operation group only received middle cerebral artery occlusion (MCAO) but without thread insertion. The MCAO model was prepared in the rest 4 groups, followed by MCAO2 h later. Ginsenoside Rb1 at each dose was peritoneally administrated to rats in corresponding groups immediately after cerebral ischemia. Equal volume of normal saline was administered to rats in the sham-operation group. Rats' cerebral infarction volume, integrals of neurologic defect degree, expression of IL-1 beta content in the brain tissue and sera were observed 24 h after 2-h cerebral I/R.
  
  
  RESULTS
  In the model group, integrals of neurologic defect degree were improved (P < 0.01), IL-1 beta positive cells in the brain tissue increased and serum IL-1 beta content elevated (P < 0.05), when compared with the sham-operation group. In comparison of the model group, integrals of neurologic defect degree were lowered in the medium dose and high dose ginsenoside Rb1 groups (P < 0.05, P < 0.01). The cerebral infarction volume was all shrunken in each ginsenoside Rb1 group, IL-1 beta positive cells in the brain tissue decreased, and IL-1 beta content in serum reduced (P < 0.01, P < 0.05). Compared with the low dose ginsenoside Rb1 group, integrals of neurologic defect degree decreased, the cerebral infarction volume shrunken, and IL-1 beta content in serum reduced in the high dose ginsenoside Rb1 group (P < 0.01, P < 0.05).
  
  
  CONCLUSIONS
  Ginsenoside Rb1 (20, 40, 80 mg/kg) might effectively release local cerebral ischemia by down-regulating the IL-1 beta expression.
authors: Department of Anesthesiology, First Affiliated Hospital, Wenzhou Medical College, Zhejiang 325000, China.
authors: Jun-Wei
authors: JW
authors: Liu
authors: Department of Anesthesiology, First Affiliated Hospital, Wenzhou Medical College, Zhejiang 325000, China.
authors: Ye-Long
authors: YL
authors: Ren
authors: Department of Anesthesiology, First Affiliated Hospital, Wenzhou Medical College, Zhejiang 325000, China.
authors: Xu-Ling
authors: XL
authors: Liu
authors: Department of Anesthesiology, First Affiliated Hospital, Wenzhou Medical College, Zhejiang 325000, China.
authors: Hong-Lian
authors: HL
authors: Xia
authors: Department of Anesthesiology, Yangquan Coal Group General Hospital, Shanxi 045000, China.
authors: Hui-Ling
authors: HL
authors: Zhang
authors: Department of Anesthesiology, First Affiliated Hospital, Wenzhou Medical College, Zhejiang 325000, China.
authors: Shen-Hui
authors: SH
authors: Jin
authors: Department of Anesthesiology, First Affiliated Hospital, Wenzhou Medical College, Zhejiang 325000, China.
authors: Qin-Xue
authors: QX
authors: Dai
authors: Department of Anesthesiology, First Affiliated Hospital, Wenzhou Medical College, Zhejiang 325000, China.
authors: Jun-Lu
authors: JL
authors: Wang
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D002545:Brain Ischemia / Q000097:blood / Q000378:metabolism*; D036145:Ginsenosides / Q000008:administration & dosage / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000097:blood / Q000378:metabolism*
chemical_list: D036145:Ginsenosides; D053583:Interleukin-1beta; C442759:ginsenoside Rb1