title: [Analysis of occurrence atypical proteins in the serum at smoking and non-smoking cigarettes patients with lung cancer].
abstract: The acting correctly immunological setting responds on every signal connected with appearing in the organism of the substance having of immunogenic properties. The smoking of cigarettes is connected with the continuous stimulation of the defensive mechanisms of the system both about the cellular character as and humoral. The important group of the proteins which he appears after immunostimulation in the intercellular space are particles built from the antigens of the various origin and antibodies. The analysis of the level of circulating immunological complexes (CIC) was the aim of the work at patients with the lung cancer and study the occurrence of the HSP-70 protein in circulating immunological complexes at patients with lung cancer smoking non-smoking cigarettes. Human serum was the analysed material, received from the blood taken from the ulnar vein from 39 patients with recognized neoplastic disease in age from 42 to 83 years ,13 non-smoking and 26 smoking. The marks of the levels of immunological complexes was conducted Haskova method and reactions on the presence of HSP-70 protein was conducted Dot Blot method by use the mice's antibodies mAbHSP-70. It was confirmed: are raised level CIC in sera of patients with lung cancer; higher levels CIC in smoking patients with lung cancer in comparison with control group and not smoking patients with lung cancer group; positive reactions on the presence of the isolated from sera of patients with lung cancer; positive reactions on the presence of the HSP-70 protein in CIC isolated from sera of smoking patients with lung cancer.
authors: Zaklad Biochemii i Biologii Komórki, Instytut Biologii Eksperymentalnej, Uniwersytet Kazimierza Wielkiego w Bydgoszczy. goldar56@gmail.com
authors: Ryszard
authors: R
authors: Gołda
authors: Katedra i Klinika Chorób Pluc, Nowotworów i Gruzlicy, Collegium Medicum im. Ludwika Rydygiera w Bydgoszczy, Uniwersytet Mikolaja Kopernika w Toruniu.
authors: Grzegorz
authors: G
authors: Przybylski
authors: Zaklad Biochemii i Biologii Komórki, Instytut Biologii Eksperymentalnej, Uniwersytet Kazimierza Wielkiego w Bydgoszczy.
authors: Wioletta
authors: W
authors: Dokładna
authors: Katedra i Klinika Rehabilitacji, Collegium Medicum im. Ludwika Rydygiera w Bydgoszczy, Uniwersytet Mikolaja Kopernika w Toruniu.
authors: Małgorzata
authors: M
authors: Pyskir
authors: Katedra i Zaklad Biofizyki, Collegium Medicum im. Ludwika Rydygiera w Bydgosszczy, Uniwersytet Mikolaja Kopernika w Toruniu.
authors: Jerzy
authors: J
authors: Pyskir
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000936:Antigen-Antibody Complex / Q000097:blood* / Q000276:immunology; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000097:blood*; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood* / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012907:Smoking / Q000009:adverse effects / Q000097:blood* / Q000276:immunology*
chemical_list: D000936:Antigen-Antibody Complex; D018840:HSP70 Heat-Shock Proteins