title: In vitro maturation, fertilization and embryo culture of oocytes obtained from vitrified auto-transplanted mouse ovary.
abstract: BACKGROUND
  The purpose of this study was to investigate the in vitro survival and developmental potential of oocytes obtained from vitrified mouse ovaries transplanted to a heterotopic site.
  
  
  METHODS
  In this experimental study, two-week-old mice were unilaterally ovariectomized after anesthesia. The ovaries were vitrified by cryotop. After two weeks, the ovaries were thawed and autotransplanted to the gluteus muscle tissue. Three weeks later the mice were killed, after which we removed and dissected the transplanted and opposite right ovaries. Cumulus oocyte complexes (COCs) and denuded oocytes were evaluated for in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro development (IVD). The control group consisted of sevenweek- old age-matched mice ovaries.
  
  
  RESULTS
  All vitrified-transplanted (Vit-trans) ovaries contained some oocytes that survived. Following IVM, IVF and IVD, there were 41.7% out of 12 cultured zygotes that reached the 8-cell stage.
  
  
  CONCLUSIONS
  Our experiment supports the progressive role of long-term graft survival after wholeovarian cryopreservation by vitrification and subsequent heterotopic transplantation. It is possible to recover viable follicles and oocytes that have the ability to develop in vitro.
authors: Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran ; Department of Biology, Faculty of Basic Sciences, Tehran University, Tehran, Iran.
authors: Arash
authors: A
authors: Behbahanian
authors: Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran ; Department of Anatomy, Faculty of Medicine, Baqiyatallah (A.S.) University of Medical Sciences, Tehran, Iran.
authors: Hossein
authors: H
authors: Eimani
authors: Department of Biology, Faculty of Basic Sciences, Tehran University, Tehran, Iran.
authors: Bahman
authors: B
authors: Zeinali
authors: Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
authors: Mojtaba
authors: M
authors: Rezazadeh Valojerdi
authors: Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
authors: Poopak
authors: P
authors: Eftekhari Yazdi
authors: Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
authors: Abdolhossein
authors: A
authors: Shahverdi
authors: Department of Genetics at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
authors: Hamid
authors: H
authors: Gourabi
authors: Department of Genetics at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
authors: Afsaneh
authors: A
authors: Golkar-Narenji
keywords: Oocytes; Ovary; Vitrification; in vitro Maturation