title: Embryonic domains of the aorta derived from diverse origins exhibit distinct properties that converge into a common phenotype in the adult.
abstract: Vascular smooth muscle cells (VSMCs) are derived from distinct embryonic origins. Vessels originating from differing smooth muscle cell populations have distinct vascular and pathological properties involving calcification, atherosclerosis, and structural defects such as aneurysm and coarctation. We hypothesized that domains within a single vessel, such as the aorta, vary in phenotype based on embryonic origin. Gene profiling and myographic analyses demonstrated that embryonic ascending and descending aortic domains exhibited distinct phenotypes. In vitro analyses demonstrated that VSMCs from each region were dissimilar in terms of cytoskeletal and migratory properties, and retention of different gene expression patterns. Using the same analysis, we found that these same two domains are indistinguishable in the adult vessel. Our data demonstrate that VSMCs from different embryonic origins are functionally distinct in the embryonic mouse, but converge to assume a common phenotype in the aorta of healthy adults. These findings have fundamental implications for aortic development, function and disease progression.
authors: Department of Cell and Developmental Biology, Vanderbilt University, 2220 Pierce Ave, PRB 348, Nashville, TN 37232, USA. Electronic address: Elise.r.pfaltzgraff@vanderbilt.edu.
authors: Elise R
authors: ER
authors: Pfaltzgraff
authors: Department of Pediatrics, Vanderbilt University, 2215 B Garland Ave., 1125 MRB IV Bldg, Nashville, TN 37232, USA. Electronic address: Elaine.l.shelton@vanderbilt.edu.
authors: Elaine L
authors: EL
authors: Shelton
authors: Department of Cardiovascular Medicine, Vanderbilt University, 2220 Pierce Ave, PRB 359, Nashville, TN 37232, USA. Electronic address: Cristi.l.galindo@vanderbilt.edu.
authors: Cristi L
authors: CL
authors: Galindo
authors: Department of Cell and Developmental Biology, Vanderbilt University, 2213 Garland Ave, MRBIV 9415, Nashville, TN 37232, USA. Electronic address: bnelms@fisk.edu.
authors: Brian L
authors: BL
authors: Nelms
authors: Department of Pediatrics, Vanderbilt University, 2215 B Garland Ave., 1125 MRB IV Bldg, Nashville, TN 37232, USA. Electronic address: Christopher.w.hooper@vanderbilt.edu.
authors: Christopher W
authors: CW
authors: Hooper
authors: Department of Pediatrics, Vanderbilt University, 2215 B Garland Ave., 1125 MRB IV Bldg, Nashville, TN 37232, USA. Electronic address: Stan.poole@vanderbilt.edu.
authors: Stanley D
authors: SD
authors: Poole
authors: Department of Cell and Developmental Biology, Vanderbilt University, 2213 Garland Ave, MRBIV 9415, Nashville, TN 37232, USA. Electronic address: Patricia.labosky@nih.gov.
authors: Patricia A
authors: PA
authors: Labosky
authors: Department of Cell and Developmental Biology, Vanderbilt University, 2220 Pierce Ave, PRB 348, Nashville, TN 37232, USA; Department of Cardiovascular Medicine, Vanderbilt University, 2220 Pierce Ave, PRB 359, Nashville, TN 37232, USA. Electronic address: David.bader@vanderbilt.edu.
authors: David M
authors: DM
authors: Bader
authors: Department of Cell and Developmental Biology, Vanderbilt University, 2213 Garland Ave, MRBIV 9415, Nashville, TN 37232, USA; Department of Pediatrics, Vanderbilt University, 2215 B Garland Ave., 1125 MRB IV Bldg, Nashville, TN 37232, USA. Electronic address: jeff.reese@vanderbilt.edu.
authors: Jeff
authors: J
authors: Reese
mesh_terms: D000818:Animals; D001011:Aorta / Q000196:embryology* / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D002454:Cell Differentiation; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology* / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D014644:Genetic Variation; D008297:Male; D051379:Mice; D009131:Muscle, Smooth, Vascular / Q000196:embryology* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger
keywords: Mesoderm; Neural crest; Smooth muscle; Vasculature