title: Enhanced polyhydroxyalkanoate production from organic wastes via process control.
abstract: This work explores the use of a model-based control scheme to enhance the productivity of polyhroxyalkanoate (PHA) production in a mixed culture two-stage system fed with synthetic wastewater. The controller supplies pulses of substrate while regulating the dissolved oxygen (DO) concentration and uses the data to fit a dynamic mathematical model, which in turn is used to predict the time until the next pulse addition. Experiments in a bench scale system first determined the optimal DO set-point and initial substrate concentration. Then the proposed feedback control strategy was compared with a simpler empiric algorithm. The results show that a substrate conversion rate of 1.370±0.598mgPHA/mgCOD/d was achieved. The proposed strategy can also indicate when to stop the accumulation of PHA upon saturation, which occurred with a PHA content of 71.0±7.2wt.%.
authors: Laboratory for Research on Advanced Processes for Water Treatment, Instituto de Ingeniería, Unidad Académica Juriquilla, Querétaro, Universidad Nacional Autónoma de México, Blvd. Juriquilla 3001, Querétaro 76230, Mexico. Electronic address: AVargasC@ii.unam.mx.
authors: Alejandro
authors: A
authors: Vargas
authors: Laboratory for Research on Advanced Processes for Water Treatment, Instituto de Ingeniería, Unidad Académica Juriquilla, Querétaro, Universidad Nacional Autónoma de México, Blvd. Juriquilla 3001, Querétaro 76230, Mexico.
authors: Liliana
authors: L
authors: Montaño
authors: Laboratory for Research on Advanced Processes for Water Treatment, Instituto de Ingeniería, Unidad Académica Juriquilla, Querétaro, Universidad Nacional Autónoma de México, Blvd. Juriquilla 3001, Querétaro 76230, Mexico.
authors: Rodolfo
authors: R
authors: Amaya
mesh_terms: D000332:Aerobiosis; D000704:Analysis of Variance; D061249:Batch Cell Culture Techniques; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D005232:Fatty Acids, Volatile / Q000032:analysis; D007700:Kinetics; D010100:Oxygen / Q000032:analysis; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*; D014866:Waste Products
chemical_list: D005232:Fatty Acids, Volatile; D054813:Polyhydroxyalkanoates; D014866:Waste Products; D010100:Oxygen
keywords: Model-based control; Optimization; Polyhydroxyalkanoate; Process control; Wastewater treatment