title: Genome-wide mRNA and miRNA analysis of peripheral blood mononuclear cells (PBMC) reveals different miRNAs regulating HIV/HCV co-infection.
abstract: Co-infection with human immunodeficiency virus (HIV) and hepatitis C virus (HCV) is common due to shared transmission routes. The genomic basis of HIV/HCV co-infection and its regulation by microRNA (miRNA) is unknown. Therefore, our objective was to investigate genome-wide mRNA expression and its regulation by miRNA in primary PBMCs derived from 27 patients (5 HCV - mono-infected, 5 HIV-mono-infected, 12 HCV/HIV co-infected, and 5 healthy controls). This revealed 27 miRNAs and 476 mRNAs as differentially expressed (DE) in HCV/HIV co-infection when compared to controls (adj p<0.05). Our study shows the first evidence of miRNAs specific for co-infection, several of which are correlated with key gene targets demonstrating functional relationships to pathways in cancer, immune-function, and metabolism. Notable was the up regulation of HCV-specific miR-122 in co-infection (FC>50, p=4.02E-06), which may have clinical/biological implications.
authors: Retroviral Genetics Division, Centre for Virus Research, Westmead Millennium Institute and Westmead Hospital, The University of Sydney, Westmead, NSW 2145, Australia.
authors: P
authors: P
authors: Gupta
authors: School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, Hunter Medical Research Institute, The University of Newcastle, University Drive, Callaghan, NSW 2308, Australia. Electronic address: BLiu@ccia.unsw.edu.au.
authors: B
authors: B
authors: Liu
authors: School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, Hunter Medical Research Institute, The University of Newcastle, University Drive, Callaghan, NSW 2308, Australia; Schizophrenia Research Institute, 405 Liverpool St, Darlinghurst, NSW 2010, Australia.
authors: J Q
authors: JQ
authors: Wu
authors: Department of Infectious Diseases, Hospital Carlos III, Sinesio Delgado 10, 28029 Madrid, Spain.
authors: V
authors: V
authors: Soriano
authors: Department of Infectious Diseases, Hospital Carlos III, Sinesio Delgado 10, 28029 Madrid, Spain.
authors: E
authors: E
authors: Vispo
authors: School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, Hunter Medical Research Institute, The University of Newcastle, University Drive, Callaghan, NSW 2308, Australia.
authors: A P
authors: AP
authors: Carroll
authors: School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, Hunter Medical Research Institute, The University of Newcastle, University Drive, Callaghan, NSW 2308, Australia; Schizophrenia Research Institute, 405 Liverpool St, Darlinghurst, NSW 2010, Australia.
authors: B J
authors: BJ
authors: Goldie
authors: School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, Hunter Medical Research Institute, The University of Newcastle, University Drive, Callaghan, NSW 2308, Australia; Schizophrenia Research Institute, 405 Liverpool St, Darlinghurst, NSW 2010, Australia. Electronic address: Murray.Cairns@newcastle.edu.au.
authors: M J
authors: MJ
authors: Cairns
authors: Retroviral Genetics Division, Centre for Virus Research, Westmead Millennium Institute and Westmead Hospital, The University of Sydney, Westmead, NSW 2145, Australia. Electronic address: nitin.saksena@sydney.edu.au.
authors: N K
authors: NK
authors: Saksena
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D060085:Coinfection / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D005260:Female; D020869:Gene Expression Profiling; D016678:Genome; D015658:HIV Infections / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D016174:Hepacivirus / Q000235:genetics / Q000502:physiology*; D006526:Hepatitis C / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged
chemical_list: D035683:MicroRNAs
keywords: Co-infection; HCV; HIV; Microarray; miRNA