title: Anti-tumor effects of gene therapy with GALV membrane fusion glycoprotein in lung adenocarcinoma.
abstract: This study examined the efficacy of gene therapy of lung adenocarcinoma using specifically controlled type I herpes simplex virus recombinant vector expressing Gibbon ape leukemia virus membrane fusion glycoprotein gene (GALV.fus). Recombinant HSV-I plasmid carrying target transgene was constructed, and recombinant viral vector was generated in Vero cells using Lipofectamine transfection. Viral vector was introduced into lung adenocarcinoma A549 cells or human fetal fibroblast HFL-I GNHu 5 cells, or inoculated into human lung adenocarcinoma xenografts in nude mice. The anti-tumor and cytotoxic effects of GALV-FMG, the transgene, were examined in these cell and animal models. Expression of GALV-FMG in xenographs achieved 100 % tumorigenicity. Recombinant HSV-I viral vector also exhibited significant tumor cell killing effect in vitro. Relative survival rates of tumor cells treated with GALV-FMG or control vectors were, respectively, 20 and 70 %. GALV.fus has a potent anti-tumor effect against lung cancer both in vitro and in vivo. This anti-tumor potential provides foundation for further studies with this vector.
authors: Department of Cardiothoracic Surgery, The Second Affiliated Hospital of Chongqing Medical University, Linjiang Road 74, Yuzhong District, Chongqing, 400010, China, drzhubing@tom.com.
authors: Bing
authors: B
authors: Zhu
authors: Jian-ru
authors: JR
authors: Yang
authors: Xin-ping
authors: XP
authors: Fu
authors: Yue-quan
authors: YQ
authors: Jiang
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology / Q000628:therapy*; D000077192:Adenocarcinoma of Lung; D000818:Animals; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D002522:Chlorocebus aethiops; D004274:DNA, Recombinant / Q000235:genetics; D015870:Gene Expression; D015316:Genetic Therapy / Q000009:adverse effects / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D018259:Herpesvirus 1, Human / Q000235:genetics*; D006801:Humans; D017989:Leukemia Virus, Gibbon Ape / Q000235:genetics*; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy*; D008297:Male; D051379:Mice; D014709:Vero Cells; D014760:Viral Fusion Proteins / Q000235:genetics*
chemical_list: D004274:DNA, Recombinant; D014760:Viral Fusion Proteins