title: Identification of more than two paternal haplotypes of the ovine fatty acid-binding protein 4 (FABP4) gene in half-sib families: evidence of intragenic meiotic recombination.
abstract: The fatty acid binding protein 4 (FABP4) plays an important role in the regulation of lipid metabolism in mammals. In this study, two regions of ovine FABP4 spanning exon 2-intron 2 and exon 3-intron 3 were investigated in four hundred and twenty lambs derived from seven sires that were previously typed as having heterozygous genotypes in both these regions of the gene. These regions have been shown to be variable, with three SNPs plus one indel and four SNPs respectively constituting five and four allele variants in the two regions. Across these regions, fourteen haplotypes have been identified. The lambs were typed using a Polymerase Chain Reaction Single-Stranded Conformational Polymorphism (PCR-SSCP) method to identify the haplotypes inherited from the sires. Between three and four paternally-derived haplotypes were identified in the progeny of six of the seven sires, suggesting that meiotic recombination occurs within ovine FABP4. A number of sequence motifs associated with recombination "hotspots" were detected in the two regions of the gene that were analyzed and these may facilitate the recombination.
authors: Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
authors: Wei
authors: W
authors: Yan
authors: Gene-Marker Laboratory, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln, New Zealand.
authors: Huitong
authors: H
authors: Zhou
authors: Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
authors: Yuzhu
authors: Y
authors: Luo
authors: Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
authors: Jiang
authors: J
authors: Hu
authors: Gene-Marker Laboratory, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln, New Zealand.
authors: Jon G H
authors: JG
authors: Hickford
mesh_terms: D000483:Alleles; D000818:Animals; D050556:Fatty Acid-Binding Proteins / Q000235:genetics*; D014644:Genetic Variation; D005838:Genotype; D006239:Haplotypes; D006579:Heterozygote; D008540:Meiosis; D020641:Polymorphism, Single Nucleotide; D011995:Recombination, Genetic; D012756:Sheep / Q000235:genetics
chemical_list: D050556:Fatty Acid-Binding Proteins