title: The involvement of CXCL11 in bone marrow-derived mesenchymal stem cell migration through human brain microvascular endothelial cells.
abstract: Bone marrow-derived mesenchymal stem cells (MSCs) transplant into the brain, where they play a potential therapeutic role in neurological diseases. However, the blood-brain barrier (BBB) is a native obstacle for MSCs entry into the brain. Little is known about the mechanism behind MSCs migration across the BBB. In the present study, we modeled the interactions between human MSCs (hMSCs) and human brain microvascular endothelial cells (HBMECs) to mimic the BBB microenvironment. Real-time PCR analysis indicated that the chemokine CXCL11 is produced by hMSCs and the chemokine receptor CXCR3 is expressed on HBMECs. Further results indicate that CXCL11 secreted by hMSCs may interact with CXCR3 on HBMECs to induce the disassembly of tight junctions through the activation of ERK1/2 signaling in the endothelium, which promotes MSCs transendothelial migration. These findings are relevant for understanding the biological responses of MSCs in BBB environments and helpful for the application of MSCs in neurological diseases.
authors: Department of Neurology, The First Hospital of China Medical University, 155 Nan Jing Northern Street, Shenyang, 110001, Liaoning, China.
authors: Yu
authors: Y
authors: Feng
authors: Hong-Mei
authors: HM
authors: Yu
authors: De-Shu
authors: DS
authors: Shang
authors: Wen-Gang
authors: WG
authors: Fang
authors: Zhi-Yi
authors: ZY
authors: He
authors: Yu-Hua
authors: YH
authors: Chen
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000378:metabolism; D001853:Bone Marrow / Q000378:metabolism; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D054371:Chemokine CXCL11 / Q000378:metabolism*; D042783:Endothelial Cells / Q000378:metabolism*; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D055806:Microvessels / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D054367:Receptors, CXCR3 / Q000378:metabolism*
chemical_list: C515202:CXCL11 protein, human; C515187:CXCR3 protein, human; D054371:Chemokine CXCL11; D054367:Receptors, CXCR3