title: Hippocampal BDNF treatment facilitates consolidation of spatial memory in spontaneous place recognition in rats.
abstract: In order to investigate the role of brain-derived neurotrophic factor (BDNF) in the consolidation of spatial memory, we examined the relationship between the increase of hippocampal BDNF and the establishment of long-term spatial memory in spontaneous place recognition test in rats. The test consisted of a sample phase, delay interval, and a test phase, and preferred exploration of the object in a novel place compared with that in a familiar place was assessed in the test phase. In experiment 1, dorsal hippocampal administration of anisomycin, a protein synthesis inhibitor, before the sample phase (20 min) abolished the preference for the novel place object in the test phase conducted 24h later. This impairment was reversed by the dorsal hippocampal BDNF treatment immediately after the sample phase, although the BDNF treatment alone did not improve performance. In experiment 2, we used a shorter sample phase condition (5 min) in which control rats did not show any preference for the novel place object in the test phase after 24h delay, and found that BDNF treatment immediately after the sample phase caused rats' significant preference for it. Results suggest an important role of hippocampal BDNF as a product of protein synthesis that is required for the consolidation of spatial memory.
authors: Institute of Psychology and Behavioral Neuroscience, University of Tsukuba, Tsukuba 305-8577, Ibaraki, Japan; Laboratory for Neural Circuitry of Memory, RIKEN Brain Science Institute, Hirosawa, Wako 351-0198, Saitama, Japan.
authors: Takaaki
authors: T
authors: Ozawa
authors: Institute of Psychology and Behavioral Neuroscience, University of Tsukuba, Tsukuba 305-8577, Ibaraki, Japan.
authors: Kazuo
authors: K
authors: Yamada
authors: Institute of Psychology and Behavioral Neuroscience, University of Tsukuba, Tsukuba 305-8577, Ibaraki, Japan. Electronic address: y-ichit@human.tsukuba.ac.jp.
authors: Yukio
authors: Y
authors: Ichitani
mesh_terms: D000818:Animals; D000841:Anisomycin / Q000494:pharmacology; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism* / Q000494:pharmacology; D005106:Exploratory Behavior / Q000187:drug effects / Q000502:physiology; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008568:Memory / Q000187:drug effects / Q000502:physiology*; D009483:Neuropsychological Tests; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D021641:Recognition, Psychology / Q000187:drug effects / Q000502:physiology*; D013028:Space Perception / Q000187:drug effects / Q000502:physiology*; D013647:Task Performance and Analysis; D013997:Time Factors
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D011500:Protein Synthesis Inhibitors; D000841:Anisomycin
keywords: BDNF; Consolidation; Hippocampus; Rats; Spatial memory; Spontaneous place recognition