title: Structure of the full-length yeast Arp7-Arp9 heterodimer.
abstract: The nuclear actin-related proteins Arp7 and Arp9 are components of the yeast SWI/SNF and RSC chromatin-remodelling complexes. The 3.1 Å resolution crystal structure reported here shows that the full-length Arp7 and Arp9 proteins exist as a dimer without a requirement for additional polypeptides. Of the 11 actin-related proteins, Arp7 and Arp9 are the only two directly demonstrated to form a dimer within this family. The Arp7-Arp9 heterodimer is unlikely to form an actin-like filament based on modelling using the structure. The Arp7-Arp9 structure reveals that its dimerization interface is not altered when bound in a complex with the SWI/SNF Snf2 HSA domain and the regulatory protein Rtt102.
authors: Institute of Molecular Biology and Biophysics, Department of Biology, ETH Zürich, Schafmattstrasse 20, ETH-Hönggerberg, CH-8093 Zürich, Switzerland.
authors: Joel
authors: J
authors: Lobsiger
authors: Institute of Molecular Biology and Biophysics, Department of Biology, ETH Zürich, Schafmattstrasse 20, ETH-Hönggerberg, CH-8093 Zürich, Switzerland.
authors: Yvonne
authors: Y
authors: Hunziker
authors: Institute of Molecular Biology and Biophysics, Department of Biology, ETH Zürich, Schafmattstrasse 20, ETH-Hönggerberg, CH-8093 Zürich, Switzerland.
authors: Timothy J
authors: TJ
authors: Richmond
mesh_terms: D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry* / Q000235:genetics; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics
chemical_list: C116139:ARP7 protein, S cerevisiae; C116140:ARP9 protein, S cerevisiae; D002352:Carrier Proteins; D002868:Chromosomal Proteins, Non-Histone; D011994:Recombinant Proteins; C586369:Rtt102 protein, S cerevisiae; C445179:SWI-SNF-B chromatin-remodeling complex; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors
keywords: Arp; RSC; SWI/SNF; actin; chromatin remodelling; nucleus