title: Effect of preoperative administration of methylpredonisolone and ulinastatin on tumor cell metastasis after surgical stress.
abstract: Using a rat laparotomy stress model, we conducted a comparative analysis of postoperative organ metastasis after administration of ulinastatin (UTI) or methylprednisolone (MP), which have an inhibitory effect on cytokine production. The subjects were classified into 4 groups: 1) minimal laparotomy group (C group), 2) major laparotomy group (L group), 3) preoperative MP intravenous administration + major laparotomy group (MP group), and 4) preoperative UTI intravenous administration + major laparotomy group (UTI group). Either MP or UTI was administered intravenously before surgery, and RI-labeled cells were injected into the portal vein immediately after laparotomy to collect tissue specimens in order to measure radiation dosage. Then, the concentrations of serum IL-2 and IL-6, liver interleukin 1 beta (IL-1β) and interleukin 10 (IL-10), and liver E-selectin were measured. In addition natural killer cell, (NK cell) activation and neoplastic nodules on the liver surface at 3 weeks after surgery were also measured. The adhesion rate of malignant cells to the liver was higher in the L group than in the C group, higher in the MP group than the L group, and lower overall in the UTI group. The concentration of IL-1β and IL-6 were decreased in the MP and UTI groups compared to the L group. IL-2 was decreased significantly in the MP group compared with the C and L groups. E-selectin expression level decreased in the UTI group compared with the L group. NK cell activation decreased in the MP group compared with the C group and L group, but no differences were observed between the UTI and L groups. The number of tumor nodules on the surface of the liver increased in the MP group compared with the L group, and decreased in the UTI group compared with the L group. Postoperative alleviation of invasive reaction was suggested in both the MP and UTI groups. However, preoperative administration of MP increased metastasis while that of UTI inhibited metastasis. MP was considered to have decreased anti-tumor immunocompetence and promoted metastasis, while UTI was considered to have inhibited the expression of adhesive molecules and decreased metastasis.
authors: Department of Surgery, Kurume University School of Medicine.
authors: Kazuya
authors: K
authors: Momosaki
authors: Nobuya
authors: N
authors: Ishibashi
authors: Shogo
authors: S
authors: Yoshida
authors: Tatsuya
authors: T
authors: Muraoka
authors: Katsuyuki
authors: K
authors: Tanaka
authors: Nobutaka
authors: N
authors: Iwakuma
authors: Yohsuke
authors: Y
authors: Oka
authors: Atsushi
authors: A
authors: Kaibara
authors: Yoshito
authors: Y
authors: Akagi
authors: Kazuo
authors: K
authors: Shirouzu
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D001201:Ascites / Q000473:pathology; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D016207:Cytokines / Q000097:blood; D004334:Drug Administration Schedule; D005938:Glucocorticoids / Q000008:administration & dosage / Q000633:toxicity*; D006023:Glycoproteins / Q000008:administration & dosage / Q000494:pharmacology*; D016867:Immunocompromised Host; D018836:Inflammation Mediators / Q000097:blood; D007813:Laparotomy / Q000009:adverse effects*; D008113:Liver Neoplasms / Q000276:immunology / Q000473:pathology* / Q000517:prevention & control*; D008114:Liver Neoplasms, Experimental / Q000517:prevention & control* / Q000556:secondary*; D008297:Male; D008775:Methylprednisolone / Q000008:administration & dosage / Q000633:toxicity*; D009368:Neoplasm Transplantation; D011300:Preoperative Care; D051381:Rats; D013997:Time Factors
chemical_list: D000970:Antineoplastic Agents; D016207:Cytokines; D005938:Glucocorticoids; D006023:Glycoproteins; D018836:Inflammation Mediators; C028665:urinastatin; D008775:Methylprednisolone