title: Vinculin binding angle in podosomes revealed by high resolution microscopy.
abstract: Podosomes are highly dynamic actin-rich adhesive structures formed predominantly by cells of the monocytic lineage, which degrade the extracellular matrix. They consist of a core of F-actin and actin-regulating proteins, surrounded by a ring of adhesion-associated proteins such as vinculin. We have characterised the structure of podosomes in macrophages, particularly the structure of the ring, using three super-resolution fluorescence microscopy techniques: stimulated emission depletion microscopy, structured illumination microscopy and localisation microscopy. Rather than being round, as previously assumed, we found the vinculin ring to be created from relatively straight strands of vinculin, resulting in a distinctly polygonal shape. The strands bind preferentially at angles between 116° and 135°. Furthermore, adjacent vinculin strands are observed nucleating at the corners of the podosomes, suggesting a mechanism for podosome growth.
authors: Randall Division of Cell and Molecular Biophysics, King's College London, London, United Kingdom ; Institute of Physical Chemistry, Abbe Center of Photonics, Friedrich-Schiller-Universität Jena, Jena, Germany.
authors: Marie
authors: M
authors: Walde
authors: Randall Division of Cell and Molecular Biophysics, King's College London, London, United Kingdom.
authors: James
authors: J
authors: Monypenny
authors: Randall Division of Cell and Molecular Biophysics, King's College London, London, United Kingdom ; Institute of Physical Chemistry, Abbe Center of Photonics, Friedrich-Schiller-Universität Jena, Jena, Germany ; Leibniz Institute of Photonic Technology, Jena, Germany.
authors: Rainer
authors: R
authors: Heintzmann
authors: Randall Division of Cell and Molecular Biophysics, King's College London, London, United Kingdom.
authors: Gareth E
authors: GE
authors: Jones
authors: Randall Division of Cell and Molecular Biophysics, King's College London, London, United Kingdom.
authors: Susan
authors: S
authors: Cox
mesh_terms: D008841:Actin Cytoskeleton; D000199:Actins / Q000378:metabolism; D002448:Cell Adhesion; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism*; D008853:Microscopy / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D016608:Talin / Q000737:chemistry; D016596:Vinculin / Q000737:chemistry*
chemical_list: D000199:Actins; D016608:Talin; D016596:Vinculin