title: Wound healing properties of a 3-D scaffold comprising soluble silkworm gland hydrolysate and human collagen.
abstract: Biomaterials that serve as scaffolds for cell proliferation and differentiation are increasingly being used in wound repair. In this study, the potential regenerative properties of a 3-D scaffold containing soluble silkworm gland hydrolysate (SSGH) and human collagen were evaluated. The scaffold was generated by solid-liquid phase separation and a freeze-drying method using a homogeneous aqueous solution. The porosity, swelling behavior, protein release, cytotoxicity, and antioxidative properties of scaffolds containing various ratios of SSGH and collagen were evaluated. SSGH/collagen scaffolds had a high porosity of 61-81% and swelling behavior studies demonstrated a 50-75% increase in swelling, along with complete protein release in the presence of phosphate-buffered saline. Cytocompatibility of the SSGH/collagen scaffold was demonstrated using mesenchymal stem cells from human umbilical cord. Furthermore, SSGH/collagen efficiently attenuated oxidative stress-induced cell damage. In an in vivo mouse model of wound healing, the SSGH/collagen scaffold accelerated wound re-epithelialization over a 15-day period. Overall, the microporous SSGH/collagen 3-D scaffold maintained optimal hydration of the exposed tissues and decreased wound healing time. These results contribute to the generation of advanced wound healing materials and may have future therapeutic implications.
authors: Department of Applied Bioscience, CHA University, Gyeonggi-do 463-836, Republic of Korea.
authors: Kyu-Oh
authors: KO
authors: Kim
authors: Department of Applied Bioscience, CHA University, Gyeonggi-do 463-836, Republic of Korea.
authors: Youngjun
authors: Y
authors: Lee
authors: Department of Applied Bioscience, CHA University, Gyeonggi-do 463-836, Republic of Korea.
authors: Jung-Wook
authors: JW
authors: Hwang
authors: Department of Applied Bioscience, CHA University, Gyeonggi-do 463-836, Republic of Korea.
authors: Hojin
authors: H
authors: Kim
authors: Department of Applied Bioscience, CHA University, Gyeonggi-do 463-836, Republic of Korea.
authors: Sun Mi
authors: SM
authors: Kim
authors: Department of Obstetrics and Gynecology, Bundang CHA Hospital, Seoul 135-081, Republic of Korea.
authors: Sung Woon
authors: SW
authors: Chang
authors: Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA, Suwon 441-100, Republic of Korea.
authors: Heui Sam
authors: HS
authors: Lee
authors: Department of Applied Bioscience, CHA University, Gyeonggi-do 463-836, Republic of Korea. Electronic address: yschoi@cha.ac.kr.
authors: Yong-Soo
authors: YS
authors: Choi
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D024042:Collagen Type I / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010316:Particle Size; D016062:Porosity; D011492:Protein Hydrolysates / Q000494:pharmacology*; D012995:Solubility; D013329:Structure-Activity Relationship; D013499:Surface Properties; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D001672:Biocompatible Materials; D024042:Collagen Type I; D011492:Protein Hydrolysates
keywords: Human collagen; Microporous scaffold; Silkworm gland hydrolysate; Wound healing