title: The genome, proteome and phylogenetic analysis of Sinorhizobium meliloti phage ΦM12, the founder of a new group of T4-superfamily phages.
abstract: Phage ΦM12 is an important transducing phage of the nitrogen-fixing rhizobial bacterium Sinorhizobium meliloti. Here we report the genome, phylogenetic analysis, and proteome of ΦM12, the first report of the genome and proteome of a rhizobium-infecting T4-superfamily phage. The structural genes of ΦM12 are most similar to T4-superfamily phages of cyanobacteria. ΦM12 is the first reported T4-superfamily phage to lack genes encoding class I ribonucleotide reductase (RNR) and exonuclease dexA, and to possess a class II coenzyme B12-dependent RNR. ΦM12's novel collection of genes establishes it as the founder of a new group of T4-superfamily phages, fusing features of cyanophages and phages of enteric bacteria.
authors: Department of Biological Science, Florida State University, Biology Unit I, 230A, 89 Chieftain Way, Tallahassee, FL 32306-4370, United States.
authors: Tess E
authors: TE
authors: Brewer
authors: Department of Biological Science, Florida State University, Biology Unit I, 230A, 89 Chieftain Way, Tallahassee, FL 32306-4370, United States; Institute of Molecular Biophysics, Florida State University, 91 Chieftan Way Tallahassee, FL 32306-4380 United States.
authors: M Elizabeth
authors: ME
authors: Stroupe
authors: Department of Biological Science, Florida State University, Biology Unit I, 230A, 89 Chieftain Way, Tallahassee, FL 32306-4370, United States. Electronic address: kmjones@bio.fsu.edu.
authors: Kathryn M
authors: KM
authors: Jones
mesh_terms: D017122:Bacteriophage T4 / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D016962:Sinorhizobium meliloti / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D020543:Proteome; D014764:Viral Proteins
keywords: Alphaproteobacteria; Bacteriophage; Class II ribonucleotide reductase; PhiM12; Proteome; Sinorhizobium meliloti; T4-like phage; gp20; gp23; ΦM12