title: A new glucomannan from Bletilla striata: structural and anti-fibrosis effects.
abstract: A novel water-soluble polysaccharide, designated Bletilla striata polysaccharide b (BSPb), was isolated from a traditional Chinese medicinal herb, Bletilla striata. The crude polysaccharide was obtained by hot water extraction and purified by ion-exchange chromatography. The molecular weight was 260 kDa as determined by HPGPC (high performance gel permeation chromatography). Monosaccharide analysis showed that BSPb contained glucose and mannose with a molar ratio of 3:1. Methylation and spectroscopic analyses with 1D and 2D NMR spectroscopy showed that the backbone of BSPb is mainly composed of (1 → 2)-linked α-D-mannopyranose and (1 → 4)-linked β-D-glucopyranose residues. In this study, BSPb plays an important role in protection against the renal fibrosis effect, which is probably mediated by down-regulated TGF-β RI, TGF-β RII, and α-SMA in vitro.
authors: Ying
authors: Y
authors: Wang
authors: Dan
authors: D
authors: Liu
authors: Shujun
authors: S
authors: Chen
authors: Yan
authors: Y
authors: Wang
authors: Huixing
authors: H
authors: Jiang
authors: Hongping
authors: H
authors: Yin
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D005355:Fibrosis / Q000517:prevention & control*; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D050527:Mesangial Cells; D015394:Molecular Structure; D029595:Orchidaceae / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; C000591471:polysaccharide b, Bletilla striata