title: Quantification of antioxidants by using chlorpromazine hydrochloride: application of the method to food and medicinal plant samples.
abstract: Chlorpromazine hydrochloride (CPH) (3-(2-chloro-phenothiazine-10-yl)-propyl] dimethylamine hydrochloride) has been the subject of a large number of studies employing a broad spectrum of oxidants, and chosen to examine the course of electron transfer reactions. We report on a method to determine the antioxidant activity of some food and medicinal plants using the oxidation of CPH by chromium(VI) to form a stable CPH radical in the 1:1 orthophosphoric acid-ethyl alcohol (OPA-EtOH) medium. The pink color of the control solution was measured at λ(max) of 530 nm. Nine standard antioxidants have been studied by this method, along with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The EC50, TEC50, antioxidant efficacy and the stoichiometric values for antioxidants have been evaluated. The radical scavenging activity expressed as EC50 ranged from 9.2 μg/mL in Camellia sinensis to 448.18 μg/mL in Cuminum cyminum. The application of a simple and versatile antioxidant capacity assay for dietary polyphenols and medicinal plant extracts, which are commonly used in Ayurveda opens its relevance in the field of antioxidant analysis.
authors: Department of Studies in Chemistry, University of Mysore.
authors: Padmarajaiah
authors: P
authors: Nagaraja
authors: Narayanan
authors: N
authors: Aradhana
authors: Aandamurthy
authors: A
authors: Suma
authors: Anantharaman
authors: A
authors: Shivakumar
authors: Nelligere Arkeshwaraiah
authors: NA
authors: Chamaraja
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D002746:Chlorpromazine / Q000737:chemistry*; D005504:Food Analysis / Q000379:methods*; D005609:Free Radicals / Q000737:chemistry; D010084:Oxidation-Reduction; D010946:Plants, Medicinal / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D012997:Solvents; D002746:Chlorpromazine