title: Prevalence of Bordetella infection in a hospital setting in niamey, niger.
abstract: Bordetella pertussis still poses an important health threat in developing countries. In Niger, notified pertussis cases are few despite the low diphtheria-tetanus-pertussis/pentavalent vaccine coverage. We aimed to estimate the prevalence of B. pertussis in children aged <5 years consulting at a pediatric ward. A 5-month study in 2011 recruited 342 children with respiratory symptoms at the National Hospital of Niamey. Nasopharyngeal aspirates were tested by culture and real-time polymerase chain reaction. Overall, 34 (11.2%) of the 305 available nasopharyngeal aspirates tested by real-time polymerase chain reaction were positive for a Bordetella spp., with an estimated prevalence of 8.2 cases per 1000 children aged <5. None was notified to the surveillance network. A single specimen was positive on culture. This study, the first to provide laboratory-confirmed data on pertussis in Niger, highlights the need to sensitize health care personnel to actively notify clinical cases and to integrate laboratory diagnosis in the existing surveillance system.
authors: Epicentre, Niamey, Niger viviane.jusot@gmail.com.
authors: Viviane
authors: V
authors: Jusot
authors: Laboratoire de Microbiologie, Centre Hospitalier Intercommunal, Créteil, France.
authors: Said
authors: S
authors: Aberrane
authors: Epicentre, Niamey, Niger.
authors: Franck
authors: F
authors: Alé
authors: Hopital National, Laboratoire de Biologie, Niamey, Niger.
authors: Boubou
authors: B
authors: Laouali
authors: Hopital National, Laboratoire de Biologie, Niamey, Niger.
authors: Issa
authors: I
authors: Moussa
authors: CERMES, Unite de Biologie, Plateforme PCR, Niamey, Niger.
authors: Sanda A
authors: SA
authors: Alio
authors: Hopital National, Niamey, Niger.
authors: Eric
authors: E
authors: Adehossi
authors: CERMES, Unite de Biologie, Plateforme PCR, Niamey, Niger.
authors: Jean-Marc
authors: JM
authors: Collard
authors: Epicentre, Paris, France.
authors: Rebecca F
authors: RF
authors: Grais
mesh_terms: D000293:Adolescent; D001885:Bordetella Infections / Q000175:diagnosis / Q000453:epidemiology* / Q000382:microbiology; D001886:Bordetella pertussis / Q000235:genetics / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006761:Hospitals / Q000706:statistics & numerical data; D006801:Humans; D007223:Infant; D008297:Male; D009305:Nasopharynx / Q000382:microbiology*; D009548:Niger / Q000453:epidemiology; D015995:Prevalence; D011446:Prospective Studies; D060888:Real-Time Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial
keywords: Niger; diagnosis; pertussis; prevalence