title: Simultaneous determination of omeprazole and their main metabolites in human urine samples by capillary electrophoresis using electrospray ionization-mass spectrometry detection.
abstract: We report a novel method for the simultaneous determination of omeprazole and their main metabolites (omeprazole sulphide, omeprazole sulphone and 5-hydroxy omeprazole) in human urine samples. For this purpose, two new capillary electrophoresis (CE) methods were developed for the simultaneous determination of target compounds, using initially diode-array for optical detection and electrospray ionization-mass spectrometry (ESI-MS) for metabolites identification and identity confirmation. A new metabolite (5-hydroxysulphide omeprazole) was identified by electrospray ionization multi-stage mass spectrometry (ESI-MS2) fragment which was then used to support the proposed chemical structure. Pharmacokinetic results using CE method were compared with those obtained when a HPLC method was used. Equivalent pharmacokinetics profiles resulted when any analytical methods were carried out.
authors: Department of Analytical Chemistry and Food Technology, Faculty of Chemistry, University of Castilla-La Mancha, Ciudad Real, Spain.
authors: Juan José Berzas
authors: JJ
authors: Nevado
authors: Department of Analytical Chemistry and Food Technology, Faculty of Chemistry, University of Castilla-La Mancha, Ciudad Real, Spain.
authors: Gregorio Castañeda
authors: GC
authors: Peñalvo
authors: Department of Analytical Chemistry and Food Technology, Faculty of Chemistry, University of Castilla-La Mancha, Ciudad Real, Spain.
authors: Rosa María Rodríguez
authors: RM
authors: Dorado
authors: Department of Analytical Chemistry and Food Technology, Faculty of Pharmacy University of Castilla-La Mancha, Albacete, Spain. Electronic address: virginia.rrobledo@uclm.es.
authors: Virginia Rodríguez
authors: VR
authors: Robledo
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D009853:Omeprazole / Q000031:analogs & derivatives / Q000737:chemistry* / Q000652:urine*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: C040449:omeprazole sulfide; C040450:omeprazole sulfone; D009853:Omeprazole
keywords: Capillary electrophoresis-mass spectrometry; Human urine; Metabolites; Omeprazole; Pharmacokinetics