title: Mechanism of the photoinduced uncaging reaction of puromycin protected by a 6-nitroveratryloxycarbonyl group.
abstract: The cleavage of a photolabile nitroveratryloxycarbonyl protecting group, which is widely used as caging group, was studied by femtosecond transient absorption spectroscopy in the visible and infrared spectral range and by flash-photolysis experiments on the longer time scale. On the basis of quantum-chemical calculations it is shown that directly after excitation, triplet absorption that is not part of the reactive pathway dominates the transient spectrum and that the molecules following the triplet pathway are trapped in a nonreactive triplet state. By contrast, photolysis proceeds from the singlet manifold. Therefore, trapping in the triplet state lowers the quantum yield of the process for this compound compared with other o-nitrobenzyl protecting groups. With our integrated approach of time-resolved UV and IR measurements and calculations, we can characterize the entire uncaging mechanism and identify the most relevant intermediate states along the reaction pathway. The final uncaging is accomplished within 32 μs.
authors: Institute of Physical and Theoretical Chemistry, Goethe University Frankfurt , Max-von-Laue Str. 7, 60438 Frankfurt/Main, Germany.
authors: Jörg
authors: J
authors: Kohl-Landgraf
authors: Florian
authors: F
authors: Buhr
authors: Daniel
authors: D
authors: Lefrancois
authors: Jan-Michael
authors: JM
authors: Mewes
authors: Harald
authors: H
authors: Schwalbe
authors: Andreas
authors: A
authors: Dreuw
authors: Josef
authors: J
authors: Wachtveitl
mesh_terms: D000042:Absorption; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D008958:Models, Molecular; D008968:Molecular Conformation; D009574:Nitro Compounds / Q000737:chemistry*; D010782:Photolysis; D011691:Puromycin / Q000737:chemistry*; D011789:Quantum Theory
chemical_list: D000900:Anti-Bacterial Agents; D009574:Nitro Compounds; D011691:Puromycin