title: Evaluation of osseointegration of titanium alloyed implants modified by plasma polymerization.
abstract: By means of plasma polymerization, positively charged, nanometre-thin coatings can be applied to implant surfaces. The aim of the present study was to quantify the adhesion of human bone cells in vitro and to evaluate the bone ongrowth in vivo, on titanium surfaces modified by plasma polymer coatings. Different implant surface configurations were examined: titanium alloy (Ti6Al4V) coated with plasma-polymerized allylamine (PPAAm) and plasma-polymerized ethylenediamine (PPEDA) versus uncoated. Shear stress on human osteoblast-like MG-63 cells was investigated in vitro using a spinning disc device. Furthermore, bone-to-implant contact (BIC) was evaluated in vivo. Custom-made conical titanium implants were inserted at the medial tibia of female Sprague-Dawley rats. After a follow-up of six weeks, the BIC was determined by means of histomorphometry. The quantification of cell adhesion showed a significantly higher shear stress for MG-63 cells on PPAAm and PPEDA compared to uncoated Ti6Al4V. Uncoated titanium alloyed implants showed the lowest BIC (40.4%). Implants with PPAAm coating revealed a clear but not significant increase of the BIC (58.5%) and implants with PPEDA a significantly increased BIC (63.7%). In conclusion, plasma polymer coatings demonstrate enhanced cell adhesion and bone ongrowth compared to uncoated titanium surfaces.
authors: Biomechanics and Implant Technology Research Laboratory, Department of Orthopaedics, University Medical Center Rostock, Doberaner Straße 142, 18057 Rostock, Germany. carolin.gabler@med.uni-rostock.de.
authors: Carolin
authors: C
authors: Gabler
authors: Biomechanics and Implant Technology Research Laboratory, Department of Orthopaedics, University Medical Center Rostock, Doberaner Straße 142, 18057 Rostock, Germany. carmen.zietz@med.uni-rostock.de.
authors: Carmen
authors: C
authors: Zietz
authors: Biomechanics and Implant Technology Research Laboratory, Department of Orthopaedics, University Medical Center Rostock, Doberaner Straße 142, 18057 Rostock, Germany. rebecca.goehler@uni-rostock.de.
authors: Rebecca
authors: R
authors: Göhler
authors: Biomechanics and Implant Technology Research Laboratory, Department of Orthopaedics, University Medical Center Rostock, Doberaner Straße 142, 18057 Rostock, Germany. a.fritsche@deru-hamburg.de.
authors: Andreas
authors: A
authors: Fritsche
authors: Biomechanics and Implant Technology Research Laboratory, Department of Orthopaedics, University Medical Center Rostock, Doberaner Straße 142, 18057 Rostock, Germany. tobias.lindner@med.uni-rostock.de.
authors: Tobias
authors: T
authors: Lindner
authors: Biomechanics and Implant Technology Research Laboratory, Department of Orthopaedics, University Medical Center Rostock, Doberaner Straße 142, 18057 Rostock, Germany. mjm@haenle.org.
authors: Maximilian
authors: M
authors: Haenle
authors: Leibniz Institute for Plasma Science and Technology (INP e.V.) Greifswald, Felix-Hausdorff-Str. 2, 17489 Greifswald, Germany. finke@inp-greifswald.de.
authors: Birgit
authors: B
authors: Finke
authors: Institute of Physics, University of Greifswald, Felix-Hausdorff-Str. 6, 17487 Greifswald, Germany. meichsner@physik.uni-greifswald.de.
authors: Jürgen
authors: J
authors: Meichsner
authors: Department of Oral and Maxillofacial Surgery, University Medical Center Rostock, Schillingallee 35, 18057 Rostock, Germany. solvig.lenz@uni-rostock.de.
authors: Solvig
authors: S
authors: Lenz
authors: Department of Oral and Maxillofacial Surgery, University Medical Center Rostock, Schillingallee 35, 18057 Rostock, Germany. bernhard.frerich@med.uni-rostock.de.
authors: Bernhard
authors: B
authors: Frerich
authors: Department of Cell Biology, University Medical Center Rostock, Schillingallee 69, 18057 Rostock, Germany. frank.luethen@med.uni-rostock.de.
authors: Frank
authors: F
authors: Lüthen
authors: Department of Cell Biology, University Medical Center Rostock, Schillingallee 69, 18057 Rostock, Germany. barbara.nebe@med.uni-rostock.de.
authors: J Barbara
authors: JB
authors: Nebe
authors: Biomechanics and Implant Technology Research Laboratory, Department of Orthopaedics, University Medical Center Rostock, Doberaner Straße 142, 18057 Rostock, Germany. rainer.bader@med.uni-rostock.de.
authors: Rainer
authors: R
authors: Bader
mesh_terms: D000497:Alloys; D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D020099:Coated Materials, Biocompatible; D005260:Female; D006801:Humans; D016348:Osseointegration; D010006:Osteoblasts / Q000378:metabolism; D058105:Polymerization; D019736:Prostheses and Implants; D051381:Rats; D014025:Titanium
chemical_list: D000497:Alloys; D020099:Coated Materials, Biocompatible; C031462:titanium alloy (TiAl6V4); D014025:Titanium