title: Comparison of primer sets and one-step reverse transcription polymerase chain reaction kits for the detection of bluetongue viral RNA.
abstract: Bluetongue virus is the etiological agent of bluetongue, one of the most important insect-transmitted animal diseases in the world. To establish a feasible diagnostic procedure for detecting the viral RNA, seven commercially available one-step RT-PCR kits in combination with three primer sets were evaluated. Results of this study showed remarkable differences in analytical sensitivity between the examined RT-PCR kits. In addition, it was found that a World Organization for Animal Health-recommended primer set may not be effective in detecting most BTV RNA.
authors: Animal Health Research Institute, 376 Chung-Cheng Road, Tamsui District, New Taipei City 25158, Taiwan. Electronic address: fanlee@mail.nvri.gov.tw.
authors: Fan
authors: F
authors: Lee
authors: Animal Health Research Institute, 376 Chung-Cheng Road, Tamsui District, New Taipei City 25158, Taiwan. Electronic address: yllin@mail.nvri.gov.tw.
authors: Yeou-Liang
authors: YL
authors: Lin
authors: Animal Health Research Institute, 376 Chung-Cheng Road, Tamsui District, New Taipei City 25158, Taiwan. Electronic address: tsaihj@ntu.edu.tw.
authors: Hsiang-Jung
authors: HJ
authors: Tsai
mesh_terms: D000818:Animals; D001819:Bluetongue / Q000175:diagnosis* / Q000821:virology; D001820:Bluetongue virus / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D011933:Reagent Kits, Diagnostic; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic
keywords: Bluetongue; Primer; Reverse transcription polymerase chain reaction