title: Radiosensitization of tumor cells through endoplasmic reticulum stress induced by PEGylated nanogel containing gold nanoparticles.
abstract: High atomic number molecules, such as gold and platinum, are known to enhance the biological effect of X-irradiation. This study was aimed to determine the radiosensitizing potential of PEGylated nanogel containing gold nanoparticles (GNG) and the cellular mechanism involved. GNG pretreatment increased the levels of reproductive cell death and apoptosis induced by X-irradiation. GNG accumulated in cytoplasm and increased the expression of endoplasmic reticulum (ER) stress-related protein. GNG suppressed the repair capacity of DNA after X-irradiation by down-regulating DNA repair-related proteins. Our results suggest that GNG radiosensitized cells by enhancing apoptosis and impairing DNA repair capacity via ER stress induction.
authors: Laboratory of Radiation Biology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
authors: Hironobu
authors: H
authors: Yasui
authors: Laboratory of Radiation Biology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
authors: Ryo
authors: R
authors: Takeuchi
authors: Laboratory of Radiation Biology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
authors: Masaki
authors: M
authors: Nagane
authors: Laboratory of Radiation Biology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
authors: Shunsuke
authors: S
authors: Meike
authors: Laboratory of Radiation Biology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
authors: Yoshinari
authors: Y
authors: Nakamura
authors: Laboratory of Radiation Biology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
authors: Tohru
authors: T
authors: Yamamori
authors: Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
authors: Yoshinori
authors: Y
authors: Ikenaka
authors: Laboratory of Anatomy, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
authors: Yasuhiro
authors: Y
authors: Kon
authors: Division of Materials Sciences, Faculty of Pure & Applied Sciences, University of Tsukuba, Tsukuba 305-8573, Japan.
authors: Hiroki
authors: H
authors: Murotani
authors: Division of Materials Sciences, Faculty of Pure & Applied Sciences, University of Tsukuba, Tsukuba 305-8573, Japan.
authors: Motoi
authors: M
authors: Oishi
authors: Division of Materials Sciences, Faculty of Pure & Applied Sciences, University of Tsukuba, Tsukuba 305-8573, Japan.
authors: Yukio
authors: Y
authors: Nagasaki
authors: Laboratory of Radiation Biology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan. Electronic address: inanami@vetmed.hokudai.ac.jp.
authors: Osamu
authors: O
authors: Inanami
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D006224:Cricetinae; D003412:Cricetulus; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D005782:Gels; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles; D051379:Mice; D011092:Polyethylene Glycols / Q000737:chemistry*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D014965:X-Rays
chemical_list: D005782:Gels; D011838:Radiation-Sensitizing Agents; D011092:Polyethylene Glycols; D006046:Gold
keywords: Apoptosis; DNA repair; Endoplasmic reticulum stress; Gold nanoparticle; Radiation