title: NF-κB activation and cell death after intracerebral hemorrhage in patients.
abstract: Nuclear factor-κB (NF-κB) plays an important role in secondary damage after intracerebral hemorrhage (ICH). We explored NF-κB activation and the relationship between NF-κB and cell death in the perihematomal brain tissue of patients after ICH. According to the interval between onset of hemorrhage and specimen collection, 53 cases of patients with basal ganglia hemorrhage were divided into six experimental groups: 0-6, 7-12, 13-24, 25-48, 49-96, and >96 h group. Brain tissues of the experimental groups and control group were collected. IL-1β, TNF-α, and NF-κB p65 expressions at the protein level were detected by immunohistochemistry. Cell death was detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) assay. All of the detection items of immunohistochemistry and TUNEL showed significant differences between the experimental groups and control group. At the protein level, nuclear NF-κB p65, IL-1β, and TNF-α achieved maximum values at 13-48, 0-24, and 13-48 h, respectively. Maximum cell death was reached at 13-48 h. NF-κB activation increased dramatically in perihematomal brain tissue after ICH. NF-κB activation was closely related with cell death and had an important function in secondary brain damage after ICH in patients.
authors: Department of Emergency Surgery, Qilu Hospital of Shandong University, Jinan, 250012, Shandong, People's Republic of China.
authors: Zeli
authors: Z
authors: Zhang
authors: Yuguang
authors: Y
authors: Liu
authors: Qibing
authors: Q
authors: Huang
authors: Yuxing
authors: Y
authors: Su
authors: Yuan
authors: Y
authors: Zhang
authors: Guanghui
authors: G
authors: Wang
authors: Feng
authors: F
authors: Li
mesh_terms: D000328:Adult; D000368:Aged; D016923:Cell Death / Q000502:physiology; D002543:Cerebral Hemorrhage / Q000150:complications / Q000201:enzymology* / Q000473:pathology*; D005260:Female; D005786:Gene Expression Regulation; D006406:Hematoma / Q000209:etiology; D006801:Humans; D020287:In Situ Nick-End Labeling; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000378:metabolism*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha