title: Novel methods for accurate identification, isolation, and genomic analysis of symptomatic microenvironments in atherosclerotic arteries.
abstract: A challenge facing surgeons is identification and selection of patients for carotid endarterectomy or coronary artery bypass/surgical intervention. While some patients with atherosclerosis develop unstable plaques liable to undergo thrombosis, others form more stable plaques and are asymptomatic. Identification of the cellular signaling mechanisms associated with production of the inflammatory, hemorrhagic lesions of mature heterogenic plaques will help significantly in our understanding of the differences in microenvironment associated with development of regions susceptible to rupture and thrombosis and may help to predict the risk of plaque rupture and guide surgical intervention to patients who will most benefit. Here, we demonstrate detailed and novel methodologies for successful and, more importantly, accurate and reproducible extraction, sampling, and analysis of micro-regions in stable and unstable coronary/carotid arteries. This information can be applied to samples from other origins and so should be useful for scientists working with micro-isolation techniques in all fields of biomedical science.
authors: School of Healthcare Science, Manchester Metropolitan University, Manchester, UK.
authors: Mark
authors: M
authors: Slevin
authors: Maribel
authors: M
authors: Baldellou
authors: Elspeth
authors: E
authors: Hill
authors: Yvonne
authors: Y
authors: Alexander
authors: Garry
authors: G
authors: McDowell
authors: Christopher
authors: C
authors: Murgatroyd
authors: Michael
authors: M
authors: Carroll
authors: Hans
authors: H
authors: Degens
authors: Jerzy
authors: J
authors: Krupinski
authors: Norma
authors: N
authors: Rovira
authors: Mohammad
authors: M
authors: Chowdhury
authors: Ferdinand
authors: F
authors: Serracino-Inglott
authors: Lina
authors: L
authors: Badimon
mesh_terms: D000818:Animals; D001158:Arteries / Q000378:metabolism / Q000473:pathology*; D050197:Atherosclerosis / Q000235:genetics / Q000473:pathology*; D005629:Frozen Sections; D020869:Gene Expression Profiling; D020043:Genes, Essential; D023281:Genomics; D006801:Humans; D007150:Immunohistochemistry; D061346:Laser Capture Microdissection; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D012333:RNA, Messenger