title: Generation of functionally mature neutrophils from induced pluripotent stem cells.
abstract: Induced pluripotent stem cells (iPSCs) are pluripotent stem cells established from somatic cells. The capability of iPSCs to differentiate into any mature cell lineage under the appropriate conditions allows for modeling of cell processes as well as disease states. Here, we describe an in vitro method for generating functional mature neutrophils from human iPSCs. We also describe assays for testing these differentiated cells for neutrophil characteristics and functions by morphology, cell surface markers, production of reactive oxygen species, microbial killing, and mobilization of neutrophils to an inflammatory site in an in vivo immunodeficient mouse infusion model.
authors: Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
authors: Colin L
authors: CL
authors: Sweeney
authors: Randall K
authors: RK
authors: Merling
authors: Uimook
authors: U
authors: Choi
authors: Debra Long
authors: DL
authors: Priel
authors: Douglas B
authors: DB
authors: Kuhns
authors: Hongmei
authors: H
authors: Wang
authors: Harry L
authors: HL
authors: Malech
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology*; D002465:Cell Movement; D006801:Humans; D016130:Immunophenotyping / Q000379:methods; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D051379:Mice; D009504:Neutrophils / Q000166:cytology* / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species