title: Evaluation of the bone healing process in an experimental tibial bone defect model in ovariectomized rats.
abstract: The aim of this study was to evaluate the influence of postmenopausal bone loss (induced by ovariectomy) in the process of bone healing in a tibial bone defect model in rats by means of histological evaluation of bone defects and the analysis of the expression of genes and proteins involved in bone consolidation. Twenty female Wistar rats (12 weeks old, weighing ±250 g) were randomly divided into two groups: control group (CG) and ovariectomized group (OG). Rats of OG were submitted to ovariectomy and after 8 weeks post-surgery, all animals were submitted to the tibial bone defect model. The main histological finding analysis revealed that ovariectomized animals showed a higher amount of granulation tissue and immature newly formed bone compared to CG. Furthermore, quantitative histological analysis showed that OG presented a significant decrease in the amount of newly formed bone (p = 0.0351). RT-PCR analysis showed no difference in Runx2, ALP, RANK, RANKL and Osterix gene expression 14-day post-surgery. Interestingly, immunohistochemical evaluation showed that Runx2 was down expressed (p = 0.0001) and RANKL was up expressed (p = 0.0022) in the OG. In conclusion, these data highlight that bone loss induced by ovariectomy causes an impairment in the capacity of bone to heal mainly probably because of alterations in the imbalance of osteoblasts and osteoclasts activities.
authors: Department of Physiotherapy, Post-Graduate Program of Biotechnology, Federal University of São Carlos (UFSCar), São Carlos, SP, Brazil, hueliton_kido@yahoo.com.br.
authors: Hueliton Wilian
authors: HW
authors: Kido
authors: Paulo Sérgio
authors: PS
authors: Bossini
authors: Carla Roberta
authors: CR
authors: Tim
authors: Nivaldo Antônio
authors: NA
authors: Parizotto
authors: Anderson Ferreira
authors: AF
authors: da Cunha
authors: Iran
authors: I
authors: Malavazi
authors: Ana Claudia Muniz
authors: AC
authors: Renno
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001861:Bone Regeneration; D001842:Bone and Bones / Q000187:drug effects / Q000473:pathology*; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D017102:Fracture Healing; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D010012:Osteogenesis; D010024:Osteoporosis / Q000378:metabolism; D010052:Ovariectomy; D053245:RANK Ligand / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013977:Tibia / Q000503:physiopathology*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D053245:RANK Ligand; C493717:Runx2 protein, rat; C495181:Sp7 protein, rat; D014157:Transcription Factors; D000469:Alkaline Phosphatase