title: Effect of dietary antioxidants on the cytostatic effect of acrylamide during copper-deficiency in Saccharomyces cerevisiae.
abstract: Acrylamide exposure increases oxidative stress and causes cytotoxicity. In order to understand the role of oxidative stress in acrylamide toxicity, we utilized Saccharomyces cerevisiae as a model organism grown in Yeast Peptone Dextrose (YPD) or Copper-Deficient Medium (CDM). Although the growth curves of yeast were comparable in these media, acrylamide treatment resulted in significant growth inhibition and colony formation only in the CDM. Copper-deficiency induced a decrease in the intracellular metallothionein levels, along with reduced Cu, Zn-SOD activity that appeared to increase the sensitivity of the yeast to the cytostatic effect of acrylamide. Increased dichlorofluorescein (DCF) fluorescence, enhanced formation of para-phenyl tertiary butyl nitrone (PBN)-hydroxyethyl adducts and a lowered reduced glutathione (GSH) content were observed under copper-deficient conditions, when challenged with acrylamide. The cytostatic effects and intracellular redox changes in response to acrylamide were ameliorated by antioxidant molecules viz. a viz. curcumin, β-carotene, vanillin and caffeic acid, which effectively decreased the oxidative stress and improved the growth recovery.
authors: Department of Biotechnology, Krishna University, Krishna Dist., Machilipatnam, Andhra Pradesh 521001, India. bodigavijayasri@gmail.com.
authors: Upendra Nadh
authors: UN
authors: Kommuguri
authors: Poorna Venkata
authors: PV
authors: Satyaprasad Pallem
authors: Sreedhar
authors: S
authors: Bodiga
authors: Vijaya Lakshmi
authors: VL
authors: Bodiga
mesh_terms: D020106:Acrylamide / Q000633:toxicity*; D000975:Antioxidants / Q000378:metabolism*; D003300:Copper / Q000172:deficiency*; D003470:Culture Media / Q000378:metabolism; D018384:Oxidative Stress; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
chemical_list: D000975:Antioxidants; D003470:Culture Media; D020106:Acrylamide; D003300:Copper