title: Biosynthesis of benzoylformic acid from benzoyl cyanide with a new bacterial isolate of Brevibacterium sp. CCZU12-1.
abstract: Brevibacterium sp. CCZU12-1 with high nitrilase activity could effectively hydrolyze benzoyl cyanide into benzoylformic acid. After the culture optimization, the preferred carbon sources, nitrogen sources, and inducer were glucose (10 g/L), a composite of peptone (10 g/L) plus yeast extract (2.5 g/L), and ε-caprolactam (2.0 mM), respectively. After the reaction optimization, the optimum reaction temperature, reaction pH, organic cosolvent, and metal ion were 30 °C, 7.0, ethanol (2%, v/v), and Ca(2+) (0.1 mM), respectively. At biotransformation of 120-mM benzoyl cyanide for 24 h, the yield of benzoylformic acid reached 91.8%. Moreover, the microbial nitrilase from Brevibacterium sp. CCZU12-1 could hydrolyze various nitriles, and it significantly exhibited high nitrilase activity against benzoyl cyanide, 3-cyanopyridine, and α-cyclohexyl-mandelonitrile.
authors: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, 213164, China, heyucai2001@163.com.
authors: Yu-Cai
authors: YC
authors: He
authors: Xue-He
authors: XH
authors: Pan
authors: Xiao-Feng
authors: XF
authors: Xu
authors: Li-Qun
authors: LQ
authors: Wang
mesh_terms: D000619:Aminohydrolases / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D055162:Biocatalysis; D001951:Brevibacterium / Q000378:metabolism*; D002209:Caprolactam / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006038:Glyoxylates / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008333:Mandelic Acids / Q000378:metabolism*; D009570:Nitriles / Q000378:metabolism*; D010461:Peptones / Q000378:metabolism; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D006038:Glyoxylates; D008333:Mandelic Acids; D009570:Nitriles; D010461:Peptones; C012482:phenylglyoxylic acid; D002209:Caprolactam; D000619:Aminohydrolases; C021867:nitrilase; D005947:Glucose