title: Brazilian propolis protects Saccharomyces cerevisiae cells against oxidative stress.
abstract: Propolis is a natural product widely used for humans. Due to its complex composition, a number of applications (antimicrobial, antiinflammatory, anesthetic, cytostatic and antioxidant) have been attributed to this substance. Using Saccharomyces cerevisiae as a eukaryotic model we investigated the mechanisms underlying the antioxidant effect of propolis from Guarapari against oxidative stress. Submitting a wild type (BY4741) and antioxidant deficient strains (ctt1Δ, sod1Δ, gsh1Δ, gtt1Δ and gtt2Δ) either to 15 mM menadione or to 2 mM hydrogen peroxide during 60 min, we observed that all strains, except the mutant sod1Δ, acquired tolerance when previously treated with 25 μg/mL of alcoholic propolis extract. Such a treatment reduced the levels of ROS generation and of lipid peroxidation, after oxidative stress. The increase in Cu/Zn-Sod activity by propolis suggests that the protection might be acting synergistically with Cu/Zn-Sod.
authors: Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors: Rafael A
authors: RA
authors: de Sá
authors: Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors: Frederico A V
authors: FA
authors: de Castro
authors: Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors: Elis C A
authors: EC
authors: Eleutherio
authors: Departamento de Química Orgânica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors: Raquel M
authors: RM
authors: de Souza
authors: Departamento de Química Orgânica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors: Joaquim F M
authors: JF
authors: da Silva
authors: Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors: Marcos D
authors: MD
authors: Pereira
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001938:Brazil; D004361:Drug Tolerance; D006861:Hydrogen Peroxide / Q000633:toxicity; D015227:Lipid Peroxidation; D018384:Oxidative Stress; D011429:Propolis / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000502:physiology*; D013482:Superoxide Dismutase / Q000032:analysis; D024483:Vitamin K 3 / Q000633:toxicity
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D024483:Vitamin K 3; D011429:Propolis; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase
keywords: Saccharomyces cerevisiae; antioxidant; oxidative stress; propolis