title: Application of statistical experimental design for optimisation of bioinsecticides production by sporeless Bacillus thuringiensis strain on cheap medium.
abstract: In order to overproduce bioinsecticides production by a sporeless Bacillus thuringiensis strain, an optimal composition of a cheap medium was defined using a response surface methodology. In a first step, a Plackett-Burman design used to evaluate the effects of eight medium components on delta-endotoxin production showed that starch, soya bean and sodium chloride exhibited significant effects on bioinsecticides production. In a second step, these parameters were selected for further optimisation by central composite design. The obtained results revealed that the optimum culture medium for delta-endotoxin production consists of 30 g L(-1) starch, 30 g L(-1) soya bean and 9 g L(-1) sodium chloride. When compared to the basal production medium, an improvement in delta-endotoxin production up to 50% was noted. Moreover, relative toxin yield of sporeless Bacillus thuringiensis S22 was improved markedly by using optimised cheap medium (148.5 mg delta-endotoxins per g starch) when compared to the yield obtained in the basal medium (94.46 mg delta-endotoxins per g starch). Therefore, the use of optimised culture cheap medium appeared to be a good alternative for a low cost production of sporeless Bacillus thuringiensis bioinsecticides at industrial scale which is of great importance in practical point of view.
authors: Team of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia.
authors: Saoussen
authors: S
authors: Ben Khedher
authors: Team of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia. ; Biological and Environmental Sciences Department, College of Arts and Sciences, Qatar University, Doha, Qatar.
authors: Samir
authors: S
authors: Jaoua
authors: Team of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia. ; Biological and Environmental Sciences Department, College of Arts and Sciences, Qatar University, Doha, Qatar.
authors: Nabil
authors: N
authors: Zouari
mesh_terms: D001413:Bacillus thuringiensis / Q000254:growth & development* / Q000378:metabolism*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D001709:Biotechnology / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D004731:Endotoxins / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000378:metabolism*; D015233:Models, Statistical; D012107:Research Design
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D003470:Culture Media; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis
keywords: cheap medium; delta-endotoxins; optimisation; response surface methodology; sporeless Bacillus thuringiensis