title: Natural laccase mediators separated from water-washed solution of steam exploded corn straw by nanofiltration and organic solvent fractionation.
abstract: Artificially synthetic mediators of laccase had the limitation of high cost and possible toxicity. The separation of natural laccase mediators from water-washed solution (WWS) of steam exploded corn straw (SECS) was studied using nano-filtration and successive organic solvents extraction. Results indicated that the UV absorption intensity of nano-filtrated WWS was significantly enhanced. The UV absorption intensity of each extractive from WWS could be ranked as ether extractive (EE)>ethyl acetate extractive (EAE)>chloroform extractive (CE). Decoloration of crystal violet catalyzed by laccase/EE was higher than that by laccase/ABTS, which was 66.95% and 61.9% at 8h, respectively. All the decoloration rates of malachite green at 60min using EE, EAE and ABTS as mediator were both more than 80%. This research would benefit for broaden the source of laccase mediator and reduce the using cost of laccase/mediator system.
authors: State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
authors: Weihua
authors: W
authors: Qiu
authors: State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
authors: Wenyan
authors: W
authors: Zhang
authors: State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China. Electronic address: hzchen@mail.ipe.ac.cn.
authors: Hongzhang
authors: H
authors: Chen
mesh_terms: D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D003116:Color; D004396:Coloring Agents / Q000378:metabolism; D005374:Filtration / Q000379:methods*; D042845:Laccase / Q000378:metabolism*; D036103:Nanotechnology / Q000379:methods*; D009930:Organic Chemicals / Q000737:chemistry*; D012996:Solutions; D012997:Solvents / Q000737:chemistry; D013227:Steam; D055454:Trametes / Q000201:enzymology; D014466:Ultraviolet Rays; D014866:Waste Products; D003313:Zea mays / Q000737:chemistry*
chemical_list: D004396:Coloring Agents; D009930:Organic Chemicals; D012996:Solutions; D012997:Solvents; D013227:Steam; D014866:Waste Products; D042845:Laccase
keywords: Nanofiltration; Natural laccase mediator; Steam exploded corn straw; Successive organic solvent extraction; Water-washed solution