title: Optimization of polyhydroxyalkanoates fermentations with on-line capacitance measurement.
abstract: The aim of this work was to provide an effective methodology for optimization of the polyhydroxyalkanoates (PHAs) fermentation with Ralstonia eutropha by the on-line capacitance measurement. The present study found the capacitance values could reflect variations of microbial morphology and viability. Furthermore, oxygen uptake rate, specific oxygen uptake rate and specific growth rate were measured in real-time and compared with the capacitance value. In addition, a fed-batch control strategy based on the on-line capacitance measurement was proposed to improve the PHAs production by 22%.
authors: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors: Lan
authors: L
authors: Li
authors: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China. Electronic address: wangzejian@ecust.edu.cn.
authors: Ze-Jian
authors: ZJ
authors: Wang
authors: Zhejiang Pharma, Group Tian An Pharmaceutical Co. Ltd., Zhejiang 315000, China.
authors: Xue-Jun
authors: XJ
authors: Chen
authors: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors: Ju
authors: J
authors: Chu
authors: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors: Ying-Ping
authors: YP
authors: Zhuang
authors: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors: Si-Liang
authors: SL
authors: Zhang
mesh_terms: D018533:Biomass; D020118:Cupriavidus necator / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D025802:Electric Capacitance; D005285:Fermentation / Q000187:drug effects; D009862:Online Systems; D010100:Oxygen / Q000378:metabolism; D010710:Phosphates / Q000494:pharmacology; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis / Q000378:metabolism*; D013997:Time Factors
chemical_list: D010710:Phosphates; D054813:Polyhydroxyalkanoates; D010100:Oxygen
keywords: On-line capacitance measurement; Optimization; PHAs; Viable biomass