title: Direct phase measurement in zonal wavefront reconstruction using multidither coherent optical adaptive technique.
abstract: In traditional zonal wavefront sensing for adaptive optics, after local wavefront gradients are obtained, the entire wavefront can be calculated by assuming that the wavefront is a continuous surface. Such an approach will lead to sub-optimal performance in reconstructing wavefronts which are either discontinuous or undersampled by the zonal wavefront sensor. Here, we report a new method to reconstruct the wavefront by directly measuring local wavefront phases in parallel using multidither coherent optical adaptive technique. This method determines the relative phases of each pupil segment independently, and thus produces an accurate wavefront for even discontinuous wavefronts. We implemented this method in an adaptive optical two-photon fluorescence microscopy and demonstrated its superior performance in correcting large or discontinuous aberrations.
authors: Rui
authors: R
authors: Liu
authors: Daniel E
authors: DE
authors: Milkie
authors: Aaron
authors: A
authors: Kerlin
authors: Bryan
authors: B
authors: MacLennan
authors: Na
authors: N
authors: Ji
mesh_terms: D000465:Algorithms; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D008858:Microscopy, Phase-Contrast / Q000379:methods*