title: Eimeria tenella rhomboid 3 has a potential role in microneme protein cleavage.
abstract: Invasion in several apicomplexan parasites, including Eimeria tenella, is accompanied by shedding of surface adhesins by intramembrane proteolysis mediated by rhomboid protease. We have previously identified E. tenella rhomboid 3 (EtROM3), but its precise role has not been elucidated. In this study, the interactions between EtROM3 and microneme (MIC) proteins were analyzed using the yeast two hybrid technique. The results showed that c-Myc-ROM3 fusion protein interacted with EtMIC4 protein in co-transformed AH109 yeasts, which was further confirmed by immunoprecipitation assay. Smaller EtMIC4 band from co-transformed cells suggested that EtROM3 was an active protease and involved in the cleavage of EtMIC4.
authors: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China; National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
authors: Jun
authors: J
authors: Zheng
authors: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China.
authors: Pengtao
authors: P
authors: Gong
authors: National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
authors: Honglin
authors: H
authors: Jia
authors: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China.
authors: Mingying
authors: M
authors: Li
authors: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China.
authors: Guocai
authors: G
authors: Zhang
authors: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China.
authors: Xichen
authors: X
authors: Zhang
authors: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. Electronic address: Jianhuali7207@163.com.
authors: Jianhua
authors: J
authors: Li
mesh_terms: D016786:Eimeria tenella / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D015800:Protozoan Proteins / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D015800:Protozoan Proteins; D012697:Serine Endopeptidases
keywords: Eimeria tenella; Interaction; Microneme; Rhomboid