title: Molecular phylogenetic and pathogenetic characterization of Fusarium solani species complex (FSSC), the cause of dry rot on potato in Iran.
abstract: Members of Fusarium solani species complex (FSSC) are common pathogens of potato, causing dry rot in the west of Iran which involved Hamedan, Kermanshah, Eilam and Kurdistan provinces. Therefore, the objectives in this study were to isolate and identify disease-causing FSSC from infected potato tubers based on the morphological and molecular characteristics. Forty-five isolates of Fusarium were obtained from potato tubers collected from the wet market in different regions of the west of Iran and identified as FSSC through morphological characters. All of the isolates were evaluated for their pathogenicity on healthy potato tubers in the planthouse. The tubers rot symptoms were observed on the 21st day after inoculation of Fusarium isolates on the tubers tested. In the tubers inoculation tests, lesion sizes were quite variable; therefore, the measurement was done to compare the depth and width of lesion expansion among the isolates. Based on the sequence data from translation elongation factor (EF-lα) gene and internal transcript spacer (ITS) regions analysis, all of the selected FSSC isolates were divided into two major groups. This is the first report on molecular identification of FSSC strains isolated from potato tubers in Iran and Fusarium falciforme was reported for the first time in Iran.
authors: Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran. Electronic address: khchehri@gmail.com.
authors: Khosrow
authors: K
authors: Chehri
authors: Department of Biotechnology and Chemical Engineering, Science and Research Branch, Islamic Azad University, Kermanshah, Iran.
authors: Hamid Reza
authors: HR
authors: Ghasempour
authors: Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran; Department of Biotechnology and Chemical Engineering, Science and Research Branch, Islamic Azad University, Kermanshah, Iran.
authors: Naser
authors: N
authors: Karimi
mesh_terms: D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005670:Fusarium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity*; D007492:Iran; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D011198:Solanum tuberosum / Q000382:microbiology*; D014774:Virulence
chemical_list: D005656:Fungal Proteins
keywords: Iran; Morphology; Pathogenicity; phylogenetic analysis; potato dry rot