title: In vivo and in vitro evaluation of sterols from Gymnema sylvestrte R. Br.
abstract: Gymnema sylvestre R. Br. is an important medicinal plant known for its antidiabetic potential. In the present study, phytosterols from G. sylvestre was identified and quantified in vivo and in vitro. Maximum callus induction was observed in MS medium supplemented with 0.5 mg L(-1) of 2, 4-D. The protein content was significantly high both in aerial plant parts and callus tissue. Phytosterols were identified using chromatographic and spectral studies. beta-sitosterol, campesterol and stigmasterol were identified both in vivo and in vitro. Lanosterol was identified only in callus culture. Phytosterols have reported for the first time in callus culture of G. sylvestre.
authors: Laboratory of Medicinal Plant Biotechnology, University of Rajasthan, Jaipur-300004, Rajasthan, India.
authors: Sharad
authors: S
authors: Vats
authors: Laboratory of Medicinal Plant Biotechnology, University of Rajasthan, Jaipur-300004, Rajasthan, India.
authors: Raka
authors: R
authors: Kamal
mesh_terms: D031104:Gymnema sylvestre / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D010840:Phytosterols / Q000302:isolation & purification / Q000378:metabolism*; D008517:Phytotherapy; D035261:Plant Components, Aerial / Q000378:metabolism; D010936:Plant Extracts / Q000302:isolation & purification / Q000378:metabolism*; D010946:Plants, Medicinal; D046509:Tissue Culture Techniques
chemical_list: D010840:Phytosterols; D010936:Plant Extracts