title: Ethylene plays an essential role in the recovery of Arabidopsis during post-anaerobiosis reoxygenation.
abstract: Ethylene is known to play an essential role in mediating hypoxic responses in plants. Here, we show that in addition to regulating hypoxic responses, ethylene also regulates cellular responses in the reoxygenation stage after anoxic treatment in Arabidopsis. We found that expression of several ethylene biosynthetic genes and ethylene-responsive factors, including ERF1 and ERF2, was induced during reoxygenation. Compared with the wild type, two ethylene-insensitive mutants (ein2-5 and ein3eil1) were more sensitive to reoxygenation and displayed damaged phenotypes during reoxygenation. To characterize the role of ethylene, we applied microarray analysis to Col-0, ein2-5 and ein3eil1 under reoxygenation conditions. Our results showed that gene transcripts involved in reactive oxygen species (ROS) detoxification, dehydration response and metabolic processes were regulated during reoxygenation. Moreover, ethylene signalling may participate in regulating these responses and maintaining the homeostasis of different phytohormones. Our work presents evidence that ethylene has distinct functions in recovery after anoxia and provides insight into the reoxygenation signalling network.
authors: Institute of Plant Biology, National Taiwan University, Taipei, 115, Taiwan; Agricultural Biotechnology Research Center, Academia Sinica, Taipei, 115, Taiwan.
authors: Kuen-Jin
authors: KJ
authors: Tsai
authors: Shu-Jen
authors: SJ
authors: Chou
authors: Ming-Che
authors: MC
authors: Shih
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D002952:Citric Acid Cycle; D003517:Cyclopentanes / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005030:Ethylenes / Q000378:metabolism*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D006706:Homeostasis; D008954:Models, Biological; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism*; D054883:Oxylipins / Q000378:metabolism; D010641:Phenotype; D010937:Plant Growth Regulators / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D036226:Seedlings / Q000235:genetics / Q000502:physiology; D015398:Signal Transduction; D013312:Stress, Physiological; D059467:Transcriptome; D014867:Water / Q000502:physiology
chemical_list: D003517:Cyclopentanes; D004268:DNA-Binding Proteins; D005030:Ethylenes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D014867:Water; C093680:ethylene-responsive element binding protein; C011006:jasmonic acid; C036216:ethylene; D010100:Oxygen
keywords: anoxia; ein2-5 and ein3eil1; re-aeration