title: Immunohistochemistry for myc predicts survival in colorectal cancer.
abstract: MYC over-expression as determined by molecular means has been reported as a favorable prognostic biomarker in colorectal carcinoma (CRC). However MYC expression analysis is not available in the routine clinical setting. We investigated whether immunohistochemistry (IHC) for the myc protein using a novel commercially available rabbit monoclonal antibody [clone Y69] which is currently in widespread clinical use for lymphoma diagnosis could be used to predict outcome in resected CRC. Myc IHC was performed on a tissue microarray (TMA) comprising a retrospective cohort of 1421 CRC patients and scored blinded as to all clinical and pathological data. IHC was also performed on a subcohort of whole section CRCs to assess staining characteristics and concordance with TMA expression. MYC over-expression was found in 980 (69%) of CRCs and was associated with tumor stage and DNA mismatch repair/BRAF status. There was substantial agreement between TMA and whole section myc IHC (kappa = 0.742, p<0.01). CRCs with MYC over-expression demonstrated improved 5-year survival (93.2% vs. 57.3%), with the effect significantly modulated by the dominant effect of tumor stage, age at diagnosis and lymphovascular space invasion status on survival. We conclude that myc status as determined by IHC alone can be used to predict overall survival in patients with CRC undergoing surgical resection.
authors: Cancer Diagnosis and Pathology Group, Kolling Institute of Medical Research, Sydney, Australia ; Histopath Pathology, North Ryde, Australia.
authors: Christopher W
authors: CW
authors: Toon
authors: Cancer Diagnosis and Pathology Group, Kolling Institute of Medical Research, Sydney, Australia ; Dept of Anatomical Pathology, SYDPATH, St Vincents Hospital, Darlinghurst, Australia.
authors: Angela
authors: A
authors: Chou
authors: Cancer Diagnosis and Pathology Group, Kolling Institute of Medical Research, Sydney, Australia ; Dept of Anatomical Pathology, Royal North Shore Hospital, St Leonards, Australia.
authors: Adele
authors: A
authors: Clarkson
authors: Dept of Anatomical Pathology, Royal North Shore Hospital, St Leonards, Australia.
authors: Keshani
authors: K
authors: DeSilva
authors: Cancer Diagnosis and Pathology Group, Kolling Institute of Medical Research, Sydney, Australia ; Dept of Anatomical Pathology, Royal North Shore Hospital, St Leonards, Australia.
authors: Michelle
authors: M
authors: Houang
authors: University of Sydney, Camperdown, Australia ; Bill Walsh Cancer Research Laboratory, Kolling Institute of Medical Research, Sydney, Australia.
authors: Joseph C Y
authors: JC
authors: Chan
authors: Cancer Diagnosis and Pathology Group, Kolling Institute of Medical Research, Sydney, Australia ; Dept of Anatomical Pathology, Royal North Shore Hospital, St Leonards, Australia.
authors: Loretta L
authors: LL
authors: Sioson
authors: University of Sydney, Camperdown, Australia ; Bill Walsh Cancer Research Laboratory, Kolling Institute of Medical Research, Sydney, Australia.
authors: Lucy
authors: L
authors: Jankova
authors: Cancer Diagnosis and Pathology Group, Kolling Institute of Medical Research, Sydney, Australia ; Dept of Anatomical Pathology, Royal North Shore Hospital, St Leonards, Australia ; University of Sydney, Camperdown, Australia.
authors: Anthony J
authors: AJ
authors: Gill
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D010641:Phenotype; D016016:Proportional Hazards Models; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism*; D016019:Survival Analysis; D046888:Tissue Array Analysis; D055815:Young Adult
chemical_list: D016271:Proto-Oncogene Proteins c-myc