title: In vitro vitamin K3 effect on conjunctival fibroblast migration and proliferation.
abstract: OBJECTIVE
  To evaluate the dose effect of vitamin K3 on wound healing mechanisms.
  
  
  METHODS
  Conjunctival fibroblasts were incubated for 24 hours. An artificial wound was made and the cells were incubated with fresh medium plus doses of vitamin K3 to be tested. Wound repair was monitored at 0, 18, 24, and 48 hours. Proliferation was measured in actively dividing cells by [(3)H]thymidine uptake. Six different groups were tested: group 1/no drugs added, group 2/ethanol 0.1%, group 3/vitamin K3 1 mg/L, group 4/vitamin K3 2 mg/L, group 5/vitamin K3 4 mg/L, and group 6/vitamin K3 6 mg/L. Each experiment was carried out in triplicate and 4 times.
  
  
  RESULTS
  There were no differences among groups at the initial time. In vitro wound repair was slower in groups 4, 5, and 6. There were no differences between control and ethanol groups and between control and vitamin K3 1 mg/L groups. Fibroblast mitogenic activity was statistically decreased in all vitamin K groups; statistical differences were found among vitamin K3 1 mg/mL and higher doses too. In groups 5 and 6, cellular toxicity was presented.
  
  
  CONCLUSIONS
  Vitamin K3 is able to inhibit fibroblast proliferation. Vitamin K3 2 mg/L or higher doses inhibit wound healing repair, exhibiting cellular toxicity at 4 and 6 mg/L.
authors: Department of Ophthalmology, Lozano Blesa University Hospital, C/San Juan Bosco 15, 50009 Zaragoza, Spain ; Aragon Institute of Health Sciences (IIS Aragon), 50009 Zaragoza, Spain.
authors: I
authors: I
authors: Pinilla
authors: Department of Ophthalmology, Hospital García Orcoyen, Navarra, 31200 Estella, Spain.
authors: L B
authors: LB
authors: Izaguirre
authors: Department of Ophthalmology, Complejo Hospitalario de Navarra, Navarra, 31008 Pamplona, Spain.
authors: F J
authors: FJ
authors: Gonzalvo
authors: Aragon Institute of Health Sciences (IIS Aragon), 50009 Zaragoza, Spain.
authors: E
authors: E
authors: Piazuelo
authors: Aragon Institute of Health Sciences (IIS Aragon), 50009 Zaragoza, Spain.
authors: M A
authors: MA
authors: Garcia-Gonzalez
authors: Aragon Institute of Health Sciences (IIS Aragon), 50009 Zaragoza, Spain ; Department of Applied Physics, Zaragoza University, 50009 Zaragoza, Spain.
authors: A I
authors: AI
authors: Sanchez-Cano
authors: Aragon Institute of Health Sciences (IIS Aragon), 50009 Zaragoza, Spain ; Department of Gastroenterology, Lozano Blesa University Hospital, C/San Juan Bosco 15, 50009 Zaragoza, Spain.
authors: F
authors: F
authors: Sopeña
mesh_terms: D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003228:Conjunctiva / Q000166:cytology*; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D024483:Vitamin K 3 / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D024483:Vitamin K 3