title: Generation and characterization of mouse monoclonal antibodies against NS4B protein of dengue virus.
abstract: Dengue virus (DENV) non-structural protein 4B (NS4B) has been demonstrated to be an attractive antiviral target. Due to its nature as an integral membrane protein, NS4B remains poorly characterized. In this study, we generated and characterized two monoclonal antibodies (mAb) that selectively bind to DENV NS4B protein. One mAb, 10-3-7, is specific for DENV-2 NS4B, and its epitope was mapped to residues 5-15 of NS4B. The other mAb, 44-4-7, cross-reacts with all the four serotypes of DENV NS4B, and its epitope was mapped to residues 141-147 of NS4B. Using the mAbs, we probed the intracellular orientation of the epitopes of NS4B by an epitope accessibility assay. The results showed that the N-terminus of NS4B is located in the ER lumen, whereas amino acids 130-148 of NS4B are located in the cytosol. The study demonstrates that the two anti-NS4B mAbs will be useful for future structural and functional analyses of DENV NS4B.
authors: Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05-01, Chromos, Singapore 138670, Singapore.
authors: Xuping
authors: X
authors: Xie
authors: Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05-01, Chromos, Singapore 138670, Singapore.
authors: Jing
authors: J
authors: Zou
authors: Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05-01, Chromos, Singapore 138670, Singapore. Electronic address: qing_yin.wang@novartis.com.
authors: Qing-Yin
authors: QY
authors: Wang
authors: Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05-01, Chromos, Singapore 138670, Singapore.
authors: Christian G
authors: CG
authors: Noble
authors: School of Biological Sciences, Nanyang Technological University, Singapore.
authors: Julien
authors: J
authors: Lescar
authors: Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05-01, Chromos, Singapore 138670, Singapore. Electronic address: pei_yong.shi@novartis.com.
authors: Pei-Yong
authors: PY
authors: Shi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D003429:Cross Reactions; D003715:Dengue / Q000276:immunology* / Q000821:virology; D003716:Dengue Virus / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D018604:Epitope Mapping; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D017361:Viral Nonstructural Proteins
keywords: Dengue virus NS4B protein; Flavivirus replication; Membrane protein