title: Chronic murine toxoplasmosis is defined by subtle changes in neuronal connectivity.
abstract: Recent studies correlate chronic Toxoplasma gondii (T. gondii) infection with behavioral changes in rodents; additionally, seropositivity in humans is reported to be associated with behavioral and neuropsychiatric diseases. In this study we investigated whether the described behavioral changes in a murine model of chronic toxoplasmosis are associated with changes in synaptic plasticity and brain neuronal circuitry. In mice chronically infected with T. gondii, magnetic resonance imaging (MRI) data analysis displayed the presence of heterogeneous lesions scattered throughout all brain areas. However, a higher density of lesions was observed within specific regions such as the somatosensory cortex (SSC). Further histopathological examination of these brain areas indicated the presence of activated resident glia and recruited immune cells accompanied by limited alterations of neuronal viability. In vivo diffusion-tensor MRI analysis of neuronal fiber density within the infected regions revealed connectivity abnormalities in the SSC. Altered fiber density was confirmed by morphological analysis of individual, pyramidal and granule neurons, showing a reduction in dendritic arbor and spine density within the SSC, as well as in the hippocampus. Evaluation of synapse efficacy revealed diminished levels of two key synaptic proteins, PSD95 and synaptophysin, within the same brain areas, indicating deficits in functionality of the synaptic neurotransmission in infected mice. Our results demonstrate that persistent T. gondii infection in a murine model results in synaptic deficits within brain structures leading to disturbances in the morphology of noninfected neurons and modified brain connectivity, suggesting a potential explanation for the behavioral and neuropsychiatric alterations.
authors: Institute of Medical Microbiology, Otto-von-Guericke University, 39120-Magdeburg, Germany.
authors: Alexandru
authors: A
authors: Parlog
authors: Laura-Adela
authors: LA
authors: Harsan
authors: Marta
authors: M
authors: Zagrebelsky
authors: Marianna
authors: M
authors: Weller
authors: Dominik
authors: D
authors: von Elverfeldt
authors: Christian
authors: C
authors: Mawrin
authors: Martin
authors: M
authors: Korte
authors: Ildiko Rita
authors: IR
authors: Dunay
mesh_terms: D000818:Animals; D001369:Axons / Q000473:pathology; D002908:Chronic Disease; D003712:Dendrites / Q000473:pathology; D000076122:Disks Large Homolog 4 Protein; D005260:Female; D051528:Guanylate Kinases / Q000378:metabolism; D006624:Hippocampus / Q000469:parasitology / Q000473:pathology / Q000503:physiopathology; D008279:Magnetic Resonance Imaging; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D009415:Nerve Net / Q000473:pathology / Q000503:physiopathology*; D017966:Pyramidal Cells / Q000378:metabolism / Q000473:pathology; D013569:Synapses / Q000378:metabolism / Q000473:pathology; D016708:Synaptophysin / Q000378:metabolism; D014124:Toxoplasmosis, Animal / Q000469:parasitology / Q000473:pathology / Q000503:physiopathology*
chemical_list: D000076122:Disks Large Homolog 4 Protein; C501447:Dlg4 protein, mouse; D008565:Membrane Proteins; D016708:Synaptophysin; D051528:Guanylate Kinases
keywords: Behavioral manipulation; Neuronal connectivity; Parasites