title: Endothelialization of electrospun polycaprolactone (PCL) small caliber vascular grafts spun from different polymer blends.
abstract: Small caliber vascular grafts represent a challenge to material scientists. In contrast to large caliber grafts, prostheses with diameter <6 mm, lead to increased hemodynamic disturbances and thrombogenic complications. Thus, endothelialization of small caliber grafts should create a compatible interface for hemodynamic processes. The purpose of our study was to compare different compositions of electrospun scaffolds with conventional ePTFE grafts with an inner diameter of 4 mm as well as different pre-coatings to create an optimized physiological interface for endothelialization. Polycaprolactone, polylactide, and polyethylenglycol (PCL/PLA and PCL/PLA/PEG) electrospun grafts and ePTFE grafts were pre-coated with blood, gelatine or fibronectin and seeded with endothelial cells from the human term placenta. Best results were obtained with fibronectin-coated PCL/PLA/PEG grafts. Here, the number of attached viable cells was 78-81% higher than on fibronectin pre-treated ePTFE grafts. Cells attached to PCL/PLA/PEG grafts appeared in physiological cobblestone morphology. Viability analysis showed a high cell viability of more than 98%. Fibronectin-coated PCL/PLA/PEG grafts may be a promising improvement to conventionally used ePTFE grafts.
authors: Institute of Cell Biology, Histology, and Embryology, Medical University Graz, Graz, Austria; Center of Biomedical Technology Department for Health Sciences and Biomedicine, Danube University Krems, Krems, Austria.
authors: D
authors: D
authors: Pfeiffer
authors: C
authors: C
authors: Stefanitsch
authors: K
authors: K
authors: Wankhammer
authors: M
authors: M
authors: Müller
authors: L
authors: L
authors: Dreyer
authors: B
authors: B
authors: Krolitzki
authors: H
authors: H
authors: Zernetsch
authors: B
authors: B
authors: Glasmacher
authors: C
authors: C
authors: Lindner
authors: A
authors: A
authors: Lass
authors: M
authors: M
authors: Schwarz
authors: W
authors: W
authors: Muckenauer
authors: I
authors: I
authors: Lang
mesh_terms: D063566:Biodegradable Plastics / Q000737:chemistry; D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005353:Fibronectins / Q000737:chemistry*; D006801:Humans; D011091:Polyesters / Q000737:chemistry*
chemical_list: D063566:Biodegradable Plastics; D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D011091:Polyesters; C016240:polycaprolactone
keywords: electrospinning; endothelial cells; graft; tissue engineering; vascular prosthesis