title: MC540 and upconverting nanocrystal coloaded polymeric liposome for near-infrared light-triggered photodynamic therapy and cell fluorescent imaging.
abstract: In clinic, the application of photodynamic therapy (PDT) in deep tissue is severely constrained by the limited penetration depth of visible light needed for activating the photosensitizer (PS). In this Article, a merocyanine 540 (MC540) and upconverting nanoparticle (UCN) coloaded functional polymeric liposome nanocarrier, (MC540 + UCN)/FPL, was designed and constructed successfully for solving this problem in PDT. Compared with the conventional approaches using UCNs absorbing PSs directly, the combination of UCN and polymeric liposome has unique advantages. The UCN core as a transducer can convert deep-penetrating near-infrared light to visible light for activating MC540. The functional polymeric liposome shell decorated with folate as a nanoshield can keep the UCN and MC540 stable, protect them from being attacked, and help them get into cells. The results show that (MC540 + UCN)/FPL is an individual nanosphere with an average size of 26 nm. MC540 can be activated to produce singlet oxygen successfully by upconverting fluorescence emitted from UCNs. After (MC540 + UCN)/FPL was modified with folate, the cell uptake efficiency increased obviously. More interestingly, in the PDT effect test, the (MC540 + UCN)/FPL nanocarrier further improved the inhibition effect on tumor cells by anchoring targeting folate and transactivating transduction peptide. Our data suggest that the (MC540 + UCN)/FPL nanocarrier may be a useful nanoplatform for future PDT treatment in deep-cancer therapy based on upconversion mechanism.
authors: Institute of Nanobiotechnology, School of Materials Science and Engineering, Tianjin University and Tianjin Key Laboratory of Composites and Functional Materials , Tianjin 300072, People's Republic of China.
authors: Hanjie
authors: H
authors: Wang
authors: Zhongyun
authors: Z
authors: Liu
authors: Sheng
authors: S
authors: Wang
authors: Chunhong
authors: C
authors: Dong
authors: Xiaoqun
authors: X
authors: Gong
authors: Peiqi
authors: P
authors: Zhao
authors: Jin
authors: J
authors: Chang
mesh_terms: D045744:Cell Line, Tumor; D002477:Cells / Q000737:chemistry* / Q000166:cytology; D006801:Humans; D007259:Infrared Rays; D008081:Liposomes / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000188:drug therapy; D010778:Photochemotherapy / Q000295:instrumentation*; D017319:Photosensitizing Agents / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011744:Pyrimidinones / Q000737:chemistry*
chemical_list: D008081:Liposomes; D017319:Photosensitizing Agents; D011108:Polymers; D011744:Pyrimidinones; C003954:merocyanine dye