title: Specific intracellular uptake of herceptin-conjugated CdSe/ZnS quantum dots into breast cancer cells.
abstract: Herceptin, a typical monoclonal antibody, was immobilized on the surface of CdSe/ZnS core-shell quantum dots (QDs) to enhance their specific interactions with breast cancer cells (SK-BR3). The mean size of the core-shell quantum dots (28 nm), as determined by dynamic light scattering, increased to 86 nm after herceptin immobilization. The in vitro cell culture experiment showed that the keratin forming cancer cells (KB) proliferated well in the presence of herceptin-conjugated QDs (QD-Her, 5 nmol/mL), whereas most of the breast cancer cells (SK-BR3) had died. To clarify the mechanism of cell death, the interaction of SK-BR3 cells with QD-Her was examined by confocal laser scanning microscopy. As a result, the QD-Her bound specifically to the membrane of SK-BR3, which became almost saturated after 6 hours incubation. This suggests that the growth signal of breast cancer cells is inhibited completely by the specific binding of herceptin to the Her-2 receptor of SK-BR3 membrane, resulting in cell death.
authors: School of Applied Chemical Engineering, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors: Seung-Jin
authors: SJ
authors: Han
authors: School of Applied Chemical Engineering, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors: Pierson
authors: P
authors: Rathinaraj
authors: School of Applied Chemical Engineering, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors: Soo-Young
authors: SY
authors: Park
authors: School of Applied Chemical Engineering, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors: Young Kyoo
authors: YK
authors: Kim
authors: School of Advanced Materials Engineering, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors: Joon Hyung
authors: JH
authors: Lee
authors: School of Applied Chemical Engineering, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors: Inn-Kyu
authors: IK
authors: Kang
authors: Department of Chemistry, Missouri University of Science and Technology, Missouri, MO 65409, USA.
authors: Jong-Sik
authors: JS
authors: Moon
authors: Department of Chemistry, Missouri University of Science and Technology, Missouri, MO 65409, USA.
authors: Jeffrey G
authors: JG
authors: Winiarz
mesh_terms: D061067:Antibodies, Monoclonal, Humanized / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology; D001943:Breast Neoplasms; D019187:Cadmium Compounds / Q000737:chemistry / Q000493:pharmacokinetics; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D006801:Humans; D010316:Particle Size; D045663:Quantum Dots / Q000737:chemistry / Q000378:metabolism*; D018036:Selenium Compounds / Q000737:chemistry / Q000493:pharmacokinetics; D017550:Spectroscopy, Fourier Transform Infrared; D013440:Sulfides / Q000737:chemistry / Q000493:pharmacokinetics; D000068878:Trastuzumab; D017967:Zinc Compounds / Q000737:chemistry / Q000493:pharmacokinetics
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D019187:Cadmium Compounds; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; C058667:cadmium selenide; C031238:zinc sulfide; D000068878:Trastuzumab