title: Isolation of a new broad spectrum antifungal polyene from Streptomyces sp. MTCC 5680.
abstract: A new polyene macrolide antibiotic PN00053 was isolated from the fermentation broth of Streptomyces sp. wild-type strain MTCC-5680. The producer strain was isolated from fertile mountain soil of Naldehra region, Himachal Pradesh, India. The compound PN00053 was purified through various steps of chromatographic techniques and bio-activity guided fractionation followed by its characterization using physiochemical properties, spectral data ((1) H-NMR, (13) C-NMR, HMBC, HSQC, and COSY) and MS analysis. PN00053 exhibited broad spectrum in vitro antifungal activity against strains of Aspergillus fumigatus (HMR), A. fumigatus ATCC 16424, Candida albicans (I.V.), C. albicans ATCC 14503, C. krusei GO6, C. glabrata HO4, Cryptococcus neoformans, Trichophyton sp. as well as fluconazole resistant strains C. krusei GO3 and C. glabrata HO5. It did not inhibit growth of gram positive and gram-negative bacteria, displaying its specificity against fungi.
  
  
  CONCLUSIONS
  PN00053 is a novel polyene macrolide isolated from a wild strain of Streptomyces sp. PM0727240 (MTCC5680), an isolate from the mountainous rocky regions of Himachal Pradesh, India. The compound is a new derivative of the antibiotic Roflamycoin [32, 33-didehydroroflamycoin (DDHR)]. It displayed broad spectrum antifungal activity against yeast and filamentous fungi. However, it did not show any antibacterial activity. The in vitro study revealed that PN00053 has better potency as compared to clinical gold standard fluconazole. The development of pathogenic resistance against the polyenes has been seldom reported. Hence, we envisage PN00053 could be a potential antifungal lead.
authors: Department of Natural products-Biology, Piramal Enterprises Limited, Mumbai, India.
authors: A
authors: A
authors: Vartak
authors: V
authors: V
authors: Mutalik
authors: R R
authors: RR
authors: Parab
authors: P
authors: P
authors: Shanbhag
authors: S
authors: S
authors: Bhave
authors: P D
authors: PD
authors: Mishra
authors: G B
authors: GB
authors: Mahajan
mesh_terms: D000898:Antibiosis; D000935:Antifungal Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001232:Aspergillus fumigatus / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects; D003455:Cryptococcus neoformans / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D015725:Fluconazole / Q000494:pharmacology; D018942:Macrolides / Q000302:isolation & purification* / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D011090:Polyenes / Q000302:isolation & purification* / Q000494:pharmacology; D012988:Soil Microbiology; D013302:Streptomyces / Q000302:isolation & purification / Q000378:metabolism*; D014249:Trichophyton / Q000187:drug effects
chemical_list: D000935:Antifungal Agents; D018942:Macrolides; C000591760:PN00053; D011090:Polyenes; D015725:Fluconazole
keywords: Actinomycetes; Streptomycetes; antibiotics; antimicrobials; pharmaceuticals