title: The changing point-spread function: single-molecule-based super-resolution imaging.
abstract: Over the past decade, many techniques for imaging systems at a resolution greater than the diffraction limit have been developed. These methods have allowed systems previously inaccessible to fluorescence microscopy to be studied and biological problems to be solved in the condensed phase. This brief review explains the basic principles of super-resolution imaging in both two and three dimensions, summarizes recent developments, and gives examples of how these techniques have been used to study complex biological systems.
authors: Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.
authors: Mathew H
authors: MH
authors: Horrocks
authors: Matthieu
authors: M
authors: Palayret
authors: David
authors: D
authors: Klenerman
authors: Steven F
authors: SF
authors: Lee
mesh_terms: D002477:Cells / Q000166:cytology*; D005456:Fluorescent Dyes; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes