title: Using flatbed scanners to collect high-resolution time-lapsed images of the arabidopsis root gravitropic response.
abstract: Research efforts in biology increasingly require use of methodologies that enable high-volume collection of high-resolution data. A challenge laboratories can face is the development and attainment of these methods. Observation of phenotypes in a process of interest is a typical objective of research labs studying gene function and this is often achieved through image capture. A particular process that is amenable to observation using imaging approaches is the corrective growth of a seedling root that has been displaced from alignment with the gravity vector. Imaging platforms used to measure the root gravitropic response can be expensive, relatively low in throughput, and/or labor intensive. These issues have been addressed by developing a high-throughput image capture method using inexpensive, yet high-resolution, flatbed scanners. Using this method, images can be captured every few minutes at 4,800 dpi. The current setup enables collection of 216 individual responses per day. The image data collected is of ample quality for image analysis applications.
authors: Department of Biology, Doane College.
authors: Halie C
authors: HC
authors: Smith
authors: Devon J
authors: DJ
authors: Niewohner
authors: Grant D
authors: GD
authors: Dewey
authors: Autumn M
authors: AM
authors: Longo
authors: Tracy L
authors: TL
authors: Guy
authors: Bradley R
authors: BR
authors: Higgins
authors: Sarah B
authors: SB
authors: Daehling
authors: Sarah C
authors: SC
authors: Genrich
authors: Christopher D
authors: CD
authors: Wentworth
authors: Tessa L
authors: TL
authors: Durham Brooks
mesh_terms: D017360:Arabidopsis / Q000502:physiology*; D018522:Gravitropism / Q000502:physiology*; D018517:Plant Roots / Q000502:physiology; D059008:Time-Lapse Imaging / Q000295:instrumentation* / Q000379:methods*