title: Simultaneous separation and determination of four phenylethanoid glycosides in rat plasma sample after oral administration of Cistanche salsa extract by microemulsion liquid chromatography.
abstract: A simple, rapid and specific method was developed to separate as well as to determine the four phenylethanoid glycosides (PhGs) (echinacoside, tubuloside B, acteoside and isoacteoside) in rat plasma after oral administration of Cistanche salsa extract by reversed phase high performance liquid chromatography using a microemulsion as the mobile phase. The separations were performed on a Zorbax Extend-C18 column at 25°C. Photodiode-array detector was conducted at 322nm and with a flow rate of 0.8mLmin(-1). The optimized microemulsion mobile phase consisted of 0.3% triethylamine in 20mM phosphoric acid at pH 6.0, 0.8% (v/v) ethyl acetate as oil phase, 1.5% (v/v) Genapol X-080 as surfactant, 2.5% (v/v) n-propanol as co-surfactant. Under the optimal conditions, the calibration curve for four PhGs was linear in the range of 10-1000ngmL(-1) with the correlation coefficients greater than 0.9994. The intra-day and inter-day precision (RSD) were below 8.64% and the limits of detection (LOD) for the four PhGs were 0.4-1.3ngmL(-1) (S/N=3). The microemulsion liquid chromatography (MELC) method was successfully applied to separate and determine the four PhGs in rat plasma after oral administration of C. salsa extract.
authors: Department of Pharmacy, Urumqi General Hospital of PLA, Urumqi Xinjiang 830000, China.
authors: Jun
authors: J
authors: Zhou
authors: Department of Pharmacy, Urumqi General Hospital of PLA, Urumqi Xinjiang 830000, China.
authors: Qiong
authors: Q
authors: Zhang
authors: Department Clinical Laboratory, No. 23 Hospital of PLA, Urumqi Xinjiang 830000, China.
authors: Jiang Bing
authors: JB
authors: Sun
authors: Department of Chemistry, Fourth Military Medical University, Xi'an, Shanxi 710032, China.
authors: Feng Qiao
authors: FQ
authors: Wang
authors: Department of Pharmacy, Urumqi General Hospital of PLA, Urumqi Xinjiang 830000, China. Electronic address: sjbzj415@163.com.
authors: Ping
authors: P
authors: Zeng
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000379:methods*; D031671:Cistanche / Q000737:chemistry*; D004355:Drug Stability; D006027:Glycosides / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results
chemical_list: D006027:Glycosides; D010936:Plant Extracts
keywords: Cistanche salsa; Microemulsion liquid chromatography; Phenylethanoid glycosides; Rat plasma