title: Coverage of diarrhoea-associated Escherichia coli isolates from different origins with two types of phage cocktails.
abstract: Eighty-nine T4-like phages from our phage collection were tested against four collections of childhood diarrhoea-associated Escherichia coli isolates representing different geographical origins (Mexico versus Bangladesh), serotypes (69 O, 27 H serotypes), pathotypes (ETEC, EPEC, EIEC, EAEC, VTEC, Shigella), epidemiological settings (community and hospitalized diarrhoea) and years of isolation. With a cocktail consisting of 3 to 14 T4-like phages, we achieved 54% to 69% coverage against predominantly EPEC isolates from Mexico, 30% to 53% against mostly ETEC isolates from a prospective survey in Bangladesh, 24% to 61% against a mixture of pathotypes isolated from hospitalized children in Bangladesh, and 60% coverage against Shigella isolates. In comparison a commercial Russian phage cocktail containing a complex mixture of many different genera of coliphages showed 19%, 33%, 50% and 90% coverage, respectively, against the four above-mentioned collections. Few O serotype-specific phages and no broad-host range phages were detected in our T4-like phage collection. Interference phenomena between the phage isolates were observed when constituting larger phage cocktails. Since the coverage of a given T4-like phage cocktail differed with geographical area and epidemiological setting, a phage composition adapted to a local situation is needed for phage therapy approaches against E. coli pathogens.
authors: Nutrition and Health Department, Nestlé Research Center, Lausanne, Switzerland.
authors: Gilles
authors: G
authors: Bourdin
authors: Armando
authors: A
authors: Navarro
authors: Shafiqul A
authors: SA
authors: Sarker
authors: Anne-C
authors: AC
authors: Pittet
authors: Firdausi
authors: F
authors: Qadri
authors: Shamima
authors: S
authors: Sultana
authors: Alejandro
authors: A
authors: Cravioto
authors: Kaisar A
authors: KA
authors: Talukder
authors: Gloria
authors: G
authors: Reuteler
authors: Harald
authors: H
authors: Brüssow
mesh_terms: D001459:Bangladesh; D001691:Biological Therapy / Q000379:methods; D003090:Coliphages / Q000254:growth & development / Q000502:physiology*; D003967:Diarrhea / Q000382:microbiology*; D004405:Dysentery, Bacillary / Q000382:microbiology; D004926:Escherichia coli / Q000145:classification / Q000302:isolation & purification / Q000821:virology*; D004927:Escherichia coli Infections / Q000382:microbiology*; D058507:Host Specificity; D006801:Humans; D008800:Mexico; D012760:Shigella / Q000302:isolation & purification / Q000821:virology; D014762:Viral Interference