title: Effects of the antihypertensive drug benidipine on osteoblast functionin vitro.
abstract: The dihydropyridine-type calcium channel blocker, benidipine (BD) has been widely used in hypertension therapy. Previous studies have demonstrated that BD has a positive effect on bone metabolism. Inspired by this promoting phenomenon, the present study investigated the effects of BD on osteoblastsin vitro. Experiments were designed and performed, including an MTT assay, reverse transcription-polymerase chain reaction, western blot analysis, alkaline phosphatase activity measurements and alizarin red S staining. The results demonstrated that BD promoted osteoblast proliferation and osteogenic differentiation at concentrations from 1×10-6to 1×10-9M by upregulating Runx2, BMP2 and OCN gene expression levels. Overall, BD at appropriate concentrations has been demonstrated to have positive effects on osteoblast function in addition to its conventional clinical usage.
authors: Department of Prosthodontics, School of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.
authors: Baixiang
authors: B
authors: Wang
authors: Department of Comprehensive Treatment, School of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.
authors: Ming
authors: M
authors: Bi
authors: Department of Prosthodontics, Zhenjiang Stomatological Hospital, Zhenjiang, Jiangsu 212002, P.R. China.
authors: Zhen
authors: Z
authors: Zhu
authors: Department of Prosthodontics, School of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.
authors: Lei
authors: L
authors: Wu
authors: Department of Prosthodontics, School of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.
authors: Jingyun
authors: J
authors: Wang
keywords: benidipine; calcium channel blockers; hypertension; osteoporosis