title: Human papillomavirus 45 genetic variation and cervical cancer risk worldwide.
abstract: Human papillomavirus 45 (HPV45) is a member of the HPV18-related alpha-7 species and accounts for approximately 5% of all cervical cancer cases worldwide. This study evaluated the genetic diversity of HPV45 and the association of HPV45 variants with the risk of cervical cancer by sequencing the entire E6 and E7 open reading frames of 300 HPV45-positive cervical samples from 36 countries. A total of 43 HPV45 sequence variants were identified that formed 5 phylogenetic sublineages, A1, A2, A3, B1, and B2, the distribution of which varied by geographical region. Among 192 cases of cervical cancer and 101 controls, the B2 sublineage was significantly overrepresented in cervical cancer, both overall and in Africa and Europe separately. We show that the sequence analysis of E6 and E7 allows the classification of HPV45 variants and that the risk of cervical cancer may differ by HPV45 variant sublineage.
  
  
  OBJECTIVE
  This work describes the largest study to date of human papillomavirus 45 (HPV45)-positive cervical samples and provides a comprehensive reference for phylogenetic classification for use in epidemiological studies of the carcinogenicity of HPV45 genetic variants, particularly as our findings suggest that the B2 sublineage of HPV45 is associated with a higher risk of cervical cancer.
authors: International Agency for Research on Cancer, Lyon, France.
authors: Alyce A
authors: AA
authors: Chen
authors: Daniëlle A M
authors: DA
authors: Heideman
authors: Debby
authors: D
authors: Boon
authors: Tarik
authors: T
authors: Gheit
authors: Peter J F
authors: PJ
authors: Snijders
authors: Massimo
authors: M
authors: Tommasino
authors: Silvia
authors: S
authors: Franceschi
authors: Gary M
authors: GM
authors: Clifford
mesh_terms: D052685:Alphapapillomavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D016022:Case-Control Studies; D005260:Female; D014644:Genetic Variation; D014943:Global Health; D006801:Humans; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000235:genetics; D030361:Papillomavirus Infections / Q000821:virology*; D010802:Phylogeny; D002583:Uterine Cervical Neoplasms / Q000821:virology*
chemical_list: D009856:Oncogene Proteins, Viral