title: Single nucleotide polymorphism-based molecular typing of M. leprae from multicase families of leprosy patients and their surroundings to understand the transmission of leprosy.
abstract: The exact mode of transmission of leprosy is not clearly understood; however, many studies have demonstrated active transmission of leprosy around a source case. Families of five active leprosy cases and their household contacts were chosen from a high endemic area in Purulia. Fifty-two soil samples were also collected from different areas of their houses. DNA was extracted from slit-skin smears (SSS) and soil samples and the Mycobacterium leprae-specific RLEP (129 bp) region was amplified using PCR. Molecular typing of M. leprae was performed for all RLEP PCR-positive samples by single nucleotide polymorphism (SNP) typing and confirmation by DNA sequencing. SSS of these five patients and six out of the total 28 contacts were PCR positive for RLEP whereas 17 soil samples out of 52 showed the presence of M. leprae DNA. SNP typing of M. leprae from all RLEP PCR-positive subjects (patients and smear-positive contacts) and 10 soil samples showed the SNP type 1 genotype. M. leprae DNA from the five leprosy patients and the six contacts was further subtyped and the D subtype was noted in all patients and contacts, except for one contact where the C subtype was identified. Typing followed by subtyping of M. leprae clearly revealed that either the contacts were infected by the patients or both patients and contacts had the same source of infection. It also revealed that the type of M. leprae in the soil in the inhabited areas where patients resided was also of the same type as that found in patients.
authors: Stanley Browne Laboratory, The Leprosy Mission, Community Hospital Nand Nagari, Delhi, India.
authors: R P
authors: RP
authors: Turankar
authors: M
authors: M
authors: Lavania
authors: V S
authors: VS
authors: Chaitanya
authors: U
authors: U
authors: Sengupta
authors: J
authors: J
authors: Darlong
authors: F
authors: F
authors: Darlong
authors: K S R
authors: KS
authors: Siva Sai
authors: R S
authors: RS
authors: Jadhav
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial; D005190:Family; D005260:Female; D016680:Genome, Bacterial; D005838:Genotype; D006801:Humans; D007918:Leprosy / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission*; D008297:Male; D008875:Middle Aged; D058889:Molecular Typing; D009166:Mycobacterium leprae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D020641:Polymorphism, Single Nucleotide; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial
keywords: Environmental samples; M. leprae; SNP typing; leprosy multicase families; transmission