title: Planarian yorkie/YAP functions to integrate adult stem cell proliferation, organ homeostasis and maintenance of axial patterning.
abstract: During adult homeostasis and regeneration, the freshwater planarian must accomplish a constant balance between cell proliferation and cell death, while also maintaining proper tissue and organ size and patterning. How these ordered processes are precisely modulated remains relatively unknown. Here we show that planarians use the downstream effector of the Hippo signaling cascade, yorkie (yki; YAP in vertebrates) to control a diverse set of pleiotropic processes in organ homeostasis, stem cell regulation, regeneration and axial patterning. We show that yki functions to maintain the homeostasis of the planarian excretory (protonephridial) system and to limit stem cell proliferation, but does not affect the differentiation process or cell death. Finally, we show that Yki acts synergistically with WNT/β-catenin signaling to repress head determination by limiting the expression domains of posterior WNT genes and that of the WNT-inhibitor notum. Together, our data show that yki is a key gene in planarians that integrates stem cell proliferation control, organ homeostasis, and the spatial patterning of tissues.
authors: The Hospital for Sick Children, Program in Developmental and Stem Cell Biology, Toronto, ON M5G 1X8, Canada.
authors: Alexander Y T
authors: AY
authors: Lin
authors: Bret J
authors: BJ
authors: Pearson
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology / Q000502:physiology; D000818:Animals; D019521:Body Patterning; D002454:Cell Differentiation; D049109:Cell Proliferation; D006706:Homeostasis; D010932:Planarians / Q000235:genetics / Q000254:growth & development* / Q000502:physiology*; D034622:RNA Interference; D012038:Regeneration / Q000235:genetics / Q000502:physiology; D015534:Trans-Activators / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D060449:Wnt Signaling Pathway
chemical_list: D015534:Trans-Activators
keywords: Differentiation; Hippo; Lophotrochozoan; Planarians; Regeneration; Schmidtea mediterranea; Stem cells; Tumor suppression; WNT; YAP; Yorkie; β-catenin