title: The regulatory impact of immune inhibitors on T cells of SD rats.
abstract: OBJECTIVE
  To observe the regulatory impact of immune inhibitors on T cells in rats.
  
  
  METHODS
  Forty SD rats were selected and randomly divided into experimental group and control group, Rapamycin (SRL) 0.4 mg/d to fill the stomach of the former one, saline lavage was used with the latter one for two weeks. Using flow cytometry to detect the two groups of rats with spleen and thymus level of CD4+ CD25+ T cells; and the spleen cells FoxP3 mRNA expression; Using ELISA method to detect TGF-β, IL-10 levels.
  
  
  RESULTS
  The peripheral blood, spleen and thymus of CD4+ CD25+ T cells accounted for the proportion of mononuclear cells were significantly higher than that of control group (P<0.05); FoxP3 mRNA expression quantity also significantly higher than the control group (P<0.05); Experimental TGF-β in rats, IL-10 levels are significantly higher than control group (P<0.05).
  
  
  CONCLUSIONS
  Immune inhibitors can regulatory CD4+ CD25+ foxp3+ T cells in rats, a single nuclear cell proportion increase, shows that it can induce rat CD4+ CD25+ foxp3+ regulatory T cells proliferation.
authors: Uropoiesis Surgical Department, First Hospital of Baoding, Hebei, China; Medicine Clinical, Hebei University College, Hebei, China. Electronic address: 13831296111zch@sina.com.
authors: Chao-Hua
authors: CH
authors: Zhang
authors: Uropoiesis Surgical Department, First Hospital of Baoding, Hebei, China.
authors: Yan
authors: Y
authors: Huang
authors: The People's Liberation Army 252 Hospital, Baoding, Hebei, China.
authors: Gang
authors: G
authors: Han
mesh_terms: D000818:Animals; D015704:CD4 Antigens / Q000378:metabolism; D051858:Forkhead Transcription Factors / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D016753:Interleukin-10 / Q000097:blood; D008297:Male; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D020123:Sirolimus / Q000494:pharmacology*; D013154:Spleen / Q000737:chemistry; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects*; D013950:Thymus Gland / Q000737:chemistry; D016212:Transforming Growth Factor beta / Q000097:blood
chemical_list: D015704:CD4 Antigens; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; C508609:IL10 protein, human; D007166:Immunosuppressive Agents; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D020123:Sirolimus
keywords: Immune inhibitors; Influence; Regulatory T cells; Research