title: Characterization of LdrA (long direct repeat A) protein of Escherichia coli.
abstract: Reminiscent of apoptotic genes in higher systems, almost all bacteria contain 'suicide' genes, which encode toxins. Toxins inhibit cell growth and may cause cell death. These are coexpressed with their cognate antitoxins from a toxin-antitoxin (TA) operon in normally growing cells. The cellular targets of toxins are diverse. The study of the TA system is important as in addition to their involvement in the normal bacterial physiology, TA systems may also play a role in bacterial pathogenicity. The long direct repeat (Ldr) family of proteins belongs to one such TA system. Here we report characterization of LdrA, the prototypical protein of the Ldr family, and show that it is highly toxic for cell growth. The data suggests that LdrA may exert toxicity by inhibiting ATP synthesis, possibly due to its localization in the cell membrane. This inhibition of energy production leads to the inhibition of biosynthetic reactions in the cell such as DNA replication, transcription and translation, and eventually cell growth.
authors: Department of Biochemistry and Molecular Biology, Robert Wood Johnson Medical School, Piscataway, N.J., USA.
authors: Yoshihiro
authors: Y
authors: Yamaguchi
authors: Narumi
authors: N
authors: Tokunaga
authors: Masayori
authors: M
authors: Inouye
authors: Sangita
authors: S
authors: Phadtare
mesh_terms: D000255:Adenosine Triphosphate / Q000037:antagonists & inhibitors; D001427:Bacterial Toxins / Q000235:genetics / Q000633:toxicity*; D004734:Energy Metabolism / Q000187:drug effects; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000633:toxicity*
chemical_list: D001427:Bacterial Toxins; D029968:Escherichia coli Proteins; C000594693:LdrA (long direct repeat A) protein, E coli; D000255:Adenosine Triphosphate