title: miR-30a suppresses breast cancer cell proliferation and migration by targeting Eya2.
abstract: Eye absent (Eya) proteins are involved in cell fate determination in a broad spectrum of cells and tissues. Aberrant expression of Eya2 has been documented in a variety of cancers and correlates with clinical outcome. However, whether microRNAs (miRNAs) can regulate Eya2 expression remains unknown. Here, we show that miR-30a represses Eya2 expression by binding to the 3'-untranslated region of Eya2. Overexpression of Eya2 in miR-30a-transfected breast cancer cells effectively rescued the inhibition of cell proliferation and migration caused by miR-30a. Knockdown of Eya2 by small-interfering RNA (siRNA) in breast cancer cells mimicked the effect induced by miR-30a and abolished the ability of miR-30a to regulate breast cancer cell proliferation and migration. The miR-30a/Eya2 axis could regulate G1/S cell cycle progression, accompanied by the modulation of expression of cell cycle-related proteins, including cyclin A, cyclin D1, cyclin E, and c-Myc. Moreover, miR-30a expression was downregulated in breast cancer patients, and negatively correlated with Eya2, which was upregulated in breast cancer patients. These data suggest that the miR-30a/Eya2 axis may play an important role in breast cancer development and progression and that miR-30a activation or Eya2 inhibition may be a useful strategy for cancer treatment.
authors: Department of Endocrinology, Chinese PLA General Hospital, Chinese PLA Medical School, Beijing, People's Republic of China; Department of Medical Molecular Biology, Beijing Institute of Biotechnology, Beijing, People's Republic of China.
authors: Jing
authors: J
authors: Fu
authors: Department of Medical Molecular Biology, Beijing Institute of Biotechnology, Beijing, People's Republic of China.
authors: Xiaojie
authors: X
authors: Xu
authors: Department of Nuclear Medicine, Peking University First Hospital, Beijing, People's Republic of China.
authors: Lei
authors: L
authors: Kang
authors: Department of Medical Molecular Biology, Beijing Institute of Biotechnology, Beijing, People's Republic of China.
authors: Liying
authors: L
authors: Zhou
authors: Department of General Surgery, 307 Hospital of PLA, Beijing, People's Republic of China.
authors: Shibin
authors: S
authors: Wang
authors: Department of Endocrinology, Chinese PLA General Hospital, Chinese PLA Medical School, Beijing, People's Republic of China.
authors: Juming
authors: J
authors: Lu
authors: Department of Medical Molecular Biology, Beijing Institute of Biotechnology, Beijing, People's Republic of China.
authors: Long
authors: L
authors: Cheng
authors: Department of Medical Molecular Biology, Beijing Institute of Biotechnology, Beijing, People's Republic of China.
authors: Zhongyi
authors: Z
authors: Fan
authors: Department of Medical Molecular Biology, Beijing Institute of Biotechnology, Beijing, People's Republic of China.
authors: Bin
authors: B
authors: Yuan
authors: Department of Endocrinology, Chinese PLA General Hospital, Chinese PLA Medical School, Beijing, People's Republic of China.
authors: Peirong
authors: P
authors: Tian
authors: Beijing Institute of Radiation Medicine, Beijing, People's Republic of China.
authors: Xiaofei
authors: X
authors: Zheng
authors: Department of General Surgery, 307 Hospital of PLA, Beijing, People's Republic of China. Electronic address: yuchengze@sina.com.
authors: Chengze
authors: C
authors: Yu
authors: Department of Medical Molecular Biology, Beijing Institute of Biotechnology, Beijing, People's Republic of China. Electronic address: yeqn66@yahoo.com.
authors: Qinong
authors: Q
authors: Ye
authors: Department of Endocrinology, Chinese PLA General Hospital, Chinese PLA Medical School, Beijing, People's Republic of China. Electronic address: metabolism301@126.com.
authors: Zhaohui
authors: Z
authors: Lv
mesh_terms: D020413:3' Untranslated Regions; D001940:Breast / Q000378:metabolism / Q000473:pathology*; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D005260:Female; D059585:G1 Phase Cell Cycle Checkpoints; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D017027:Protein Tyrosine Phosphatases / Q000235:genetics* / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; D047908:Intracellular Signaling Peptides and Proteins; C554019:MIRN130 microRNA, human; D035683:MicroRNAs; D009687:Nuclear Proteins; C106938:EYA2 protein, human; D017027:Protein Tyrosine Phosphatases
keywords: Breast cancer; Cell migration; Cell proliferation; Eya2; miR-30a