title: Preparation and in vitro immunomodulatory effect of curdlan sulfate.
abstract: Different curdlan sulfates were synthesized by modification of curdlan with sulfur trioxide-pyridine complex, and the one with the highest sulfur content (9.23 ± 0.16) %, CS3, was used to study the immunomodulating effect on murine RAW264.7 macrophages and bone marrow derived dendritic cells (BMDCs). The results showed that the treatment of macrophages with CS3 could not only increase the nitric oxide (NO) release and the cytokines TNF-α, IL-6 and IL-1β production significantly, but also enhance the inducible NOS (iNOS) expression, NF-κBp65 nuclear translocation, Erk1/2 and SAPK/JNK phosphorylation. The combination of CS3 with GM-CSF upregulated immature BMDCs to express major histocompatibility complex II (MHCII) and CD11c surface markers, CD40, CD80 and CD86 costimulatory molecules, as well as the cytokines of IL-12p70 and IL-6. The surface plasmon resonance (SPR) analysis found that CS3 exhibited binding affinity against dectin-1. These results suggested that curdlan sulfate can possibly be developed as a new immunotherapy agent and anti-viral vaccine adjuvant.
authors: Key Laboratory of Chemical Biology of Natural Products (Ministry of Education), Institute of Biochemical and Biotechnological Drug, School of Pharmaceutical Sciences, Shandong University, Jinan 250012, Shandong, PR China; National Glycoengineering Research Center, Shandong University, Jinan 250012, Shandong, PR China.
authors: Pingli
authors: P
authors: Li
authors: Department of Pharmacology, School of Pharmaceutical Sciences, Shandong University, Jinan 250012, Shandong, PR China.
authors: Xinke
authors: X
authors: Zhang
authors: Department of Pharmacology, School of Pharmaceutical Sciences, Shandong University, Jinan 250012, Shandong, PR China.
authors: Yanna
authors: Y
authors: Cheng
authors: Department of Pharmacy, The Second Hospital of Shandong University, Jinan 250033, PR China.
authors: Juan
authors: J
authors: Li
authors: Key Laboratory of Chemical Biology of Natural Products (Ministry of Education), Institute of Biochemical and Biotechnological Drug, School of Pharmaceutical Sciences, Shandong University, Jinan 250012, Shandong, PR China.
authors: Yuliang
authors: Y
authors: Xiao
authors: Key Laboratory of Chemical Biology of Natural Products (Ministry of Education), Institute of Biochemical and Biotechnological Drug, School of Pharmaceutical Sciences, Shandong University, Jinan 250012, Shandong, PR China.
authors: Qian
authors: Q
authors: Zhang
authors: Key Laboratory of Chemical Biology of Natural Products (Ministry of Education), Institute of Biochemical and Biotechnological Drug, School of Pharmaceutical Sciences, Shandong University, Jinan 250012, Shandong, PR China.
authors: Aizhen
authors: A
authors: Zong
authors: Key Laboratory of Chemical Biology of Natural Products (Ministry of Education), Institute of Biochemical and Biotechnological Drug, School of Pharmaceutical Sciences, Shandong University, Jinan 250012, Shandong, PR China.
authors: Chuanqing
authors: C
authors: Zhong
authors: Key Laboratory of Chemical Biology of Natural Products (Ministry of Education), Institute of Biochemical and Biotechnological Drug, School of Pharmaceutical Sciences, Shandong University, Jinan 250012, Shandong, PR China; National Glycoengineering Research Center, Shandong University, Jinan 250012, Shandong, PR China. Electronic address: fswang@sdu.edu.cn.
authors: Fengshan
authors: F
authors: Wang
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D066298:In Vitro Techniques; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D047071:beta-Glucans / Q000494:pharmacology*
chemical_list: D000276:Adjuvants, Immunologic; D016207:Cytokines; D047071:beta-Glucans; D009569:Nitric Oxide; C063130:curdlan sulfate
keywords: BMDC; Curdlan sulfate; Dectin-1; Macrophage; Surface plasmon resonance