title: Biodistribution of protoporphyrin IX in female genital erosive lichen planus after topical application of hexaminolevulinate.
abstract: Genital erosive lichen planus (GELP) is a chronic inflammatory disease, in women characterized by painful vulvar and vaginal erosions. To prepare for a clinical trial on photodynamic treatment (PDT), we applied hexyl 5-aminolevulinate hydrochloride (HAL) in clinically normal and affected mucosa in 12 women with GELP using two different doses (6.25 or 50mg/ml). Biopsies were taken after 30 min and 3h. The biodistribution of HAL, measured as photoactive protoporphyrin IX (PpIX), was studied using non-invasive superficial fluorescence measurements and microscopic fluorescence photometry. More PpIX was detected after application of 12.5mg HAL than after 100mg, with large inter-individual variations. PpIX levels after 3h were overall higher than after 30 min. PpIX fluorescence was not detected in skin distant to the genital area. In conclusion, 6.25mg/ml HAL applied for 3h seems adequate for HAL absorption and conversion to PpIX in submucosal inflammatory and epithelial cells and can be used in a PDT trial of GELP.
authors: National Resource Centre for Women's Health, Division for Women's and Children's Health, Oslo University Hospital, 0424 Oslo, Norway; Department of Dermatology, Oslo University Hospital, 0424 Oslo, Norway; Institute for Clinical Medicine, University of Oslo, Box 1171 Blindern, 0318 Oslo, Norway. Electronic address: anneliseord@yahoo.no.
authors: Anne Lise Ording
authors: AL
authors: Helgesen
authors: Department of Dermatology, Oslo University Hospital, 0424 Oslo, Norway; Institute for Clinical Medicine, University of Oslo, Box 1171 Blindern, 0318 Oslo, Norway.
authors: Petter
authors: P
authors: Gjersvik
authors: Department of Pathology, Oslo University Hospital, 0424 Oslo, Norway.
authors: Qian
authors: Q
authors: Peng
authors: Department of Pathology, Oslo University Hospital, 0424 Oslo, Norway.
authors: Vlada
authors: V
authors: Vasovic
authors: Department of Biostatistics and Epidemiology, Oslo University Hospital, 0424 Oslo, Norway.
authors: Are Hugo
authors: AH
authors: Pripp
authors: Department of Pathology, Oslo University Hospital, 0424 Oslo, Norway.
authors: Peter
authors: P
authors: Jebsen
authors: Institute for Clinical Medicine, University of Oslo, Box 1171 Blindern, 0318 Oslo, Norway; Department of Gynaecology, Oslo University Hospital, 0424 Oslo, Norway.
authors: Tom
authors: T
authors: Tanbo
authors: Department of Internal Medicine, Oslo University Hospital, 0424 Oslo, Norway.
authors: Trond
authors: T
authors: Warloe
mesh_terms: D000287:Administration, Topical; D000622:Aminolevulinic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D004305:Dose-Response Relationship, Drug; D005260:Female; D005831:Genital Diseases, Female / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008010:Lichen Planus / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D008657:Metabolic Clearance Rate / Q000187:drug effects; D010778:Photochemotherapy / Q000379:methods; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000493:pharmacokinetics; D011524:Protoporphyrins / Q000378:metabolism*; D014018:Tissue Distribution / Q000187:drug effects; D016896:Treatment Outcome
chemical_list: D017319:Photosensitizing Agents; D011524:Protoporphyrins; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX; C419924:5-aminolevulinic acid hexyl ester
keywords: Erosive lichen planus; Genital; Hexylaminolevulinate