title: A skin substitute based on human amniotic membrane.
abstract: Human amniotic membrane (HAM) has biological properties which are useful for wound healing. HAM is notably one of the therapeutic alternatives for venous leg ulcer care. Indeed, a prospective clinical study has demonstrated that cryopreserved HAM transplantation for leg ulcer is feasible, safe and has beneficial effects: 80 % of the patients had a significant clinical response. Nevertheless, at the end of the 3-month follow-up period, only 20 % of the ulcers were totally closed. The aim of this work was to create and characterize a model of epidermized HAM. The method of HAM desepithelialization was validated by histology, immunohistochemistry and scanning electron microscopy. Then, de-epithelialized HAM was seeded with primary keratinocytes. After 21 days of culture, 15 at the air-liquid interface, the model obtained was analyzed histologically and by immunohistochemistry. The amniotic basement membrane was preserved during enzymatic desepithelialization of HAM. Primary keratinocytes proliferated on HAM: the model obtained showed involucrin expression and had a good basement membrane. As re-epithelialization is an important step for ulcer closure, a model of epidermized HAM could be used to speed up the healing of such wounds.
authors: UMR 1098, SFR FED 4234, University of Franche Comté, 25000, Besançon, France, htauzin@yahoo.fr.
authors: Hélène
authors: H
authors: Tauzin
authors: Gwenaël
authors: G
authors: Rolin
authors: Céline
authors: C
authors: Viennet
authors: Philippe
authors: P
authors: Saas
authors: Philippe
authors: P
authors: Humbert
authors: Patrice
authors: P
authors: Muret
mesh_terms: D000650:Amnion / Q000166:cytology*; D001485:Basement Membrane / Q000166:cytology; D006801:Humans; D015603:Keratinocytes / Q000166:cytology; D007871:Leg Ulcer / Q000628:therapy*; D012867:Skin / Q000166:cytology*; D017768:Skin, Artificial; D046509:Tissue Culture Techniques; D014945:Wound Healing / Q000502:physiology*