title: Short RNA indicator sequences are not completely degraded by autoclaving.
abstract: Short indicator RNA sequences (<100 bp) persist after autoclaving and are recovered intact by molecular amplification. Primers targeting longer sequences are most likely to produce false positives due to amplification errors easily verified by melting curves analyses. If short indicator RNA sequences are used for virus identification and quantification then post autoclave RNA degradation methodology should be employed, which may include further autoclaving.
authors: Department of Biology, New Mexico State University, Las Cruces, NM, USA, 88003.
authors: Veena V
authors: VV
authors: Unnithan
authors: 1] Plant and Environmental Sciences, New Mexico State University, Las Cruces, NM, USA, 88003 [2] Memorial University of Newfoundland, Boreal Ecosystems Research Initiative, Corner Brook, NL, Canada, A2H 6P9.
authors: Adrian
authors: A
authors: Unc
authors: Department of Biology, New Mexico State University, Las Cruces, NM, USA, 88003.
authors: Valerisa
authors: V
authors: Joe
authors: Department of Biology, New Mexico State University, Las Cruces, NM, USA, 88003.
authors: Geoffrey B
authors: GB
authors: Smith
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000378:metabolism; D006358:Hot Temperature; D017909:Levivirus / Q000235:genetics; D012367:RNA, Viral / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013242:Sterilization
chemical_list: D017931:DNA Primers; D012367:RNA, Viral