title: Umbilical cord mesenchymal stem cells labeled with multimodal iron oxide nanoparticles with fluorescent and magnetic properties: application for in vivo cell tracking.
abstract: Here we describe multimodal iron oxide nanoparticles conjugated to Rhodamine-B (MION-Rh), their stability in culture medium, and subsequent validation of an in vitro protocol to label mesenchymal stem cells from umbilical cord blood (UC-MSC) with MION-Rh. These cells showed robust labeling in vitro without impairment of their functional properties, the viability of which were evaluated by proliferation kinetic and ultrastructural analyzes. Thus, labeled cells were infused into striatum of adult male rats of animal model that mimic late onset of Parkinson's disease and, after 15 days, it was observed that cells migrated along the medial forebrain bundle to the substantia nigra as hypointense spots in T2 magnetic resonance imaging. These data were supported by short-term magnetic resonance imaging. Studies were performed in vivo, which showed that about 5 × 10(5) cells could be efficiently detected in the short term following infusion. Our results indicate that these labeled cells can be efficiently tracked in a neurodegenerative disease model.
authors: Hospital Israelita Albert Einstein, São Paulo, Brazil ; Departamento de Neurologia e Neurociências, Universidade Federal de São Paulo, São Paulo, Brazil.
authors: Tatiana T
authors: TT
authors: Sibov
authors: Hospital Israelita Albert Einstein, São Paulo, Brazil ; Departamento de Neurologia e Neurociências, Universidade Federal de São Paulo, São Paulo, Brazil.
authors: Lorena F
authors: LF
authors: Pavon
authors: Hospital Israelita Albert Einstein, São Paulo, Brazil.
authors: Liza A
authors: LA
authors: Miyaki
authors: Hospital Israelita Albert Einstein, São Paulo, Brazil.
authors: Javier B
authors: JB
authors: Mamani
authors: Hospital Israelita Albert Einstein, São Paulo, Brazil ; Departamento de Neurologia e Neurociências, Universidade Federal de São Paulo, São Paulo, Brazil.
authors: Leopoldo P
authors: LP
authors: Nucci
authors: Hospital Israelita Albert Einstein, São Paulo, Brazil ; Faculdade de Ciências Médicas da Santa Casa de São Paulo, São Paulo, Brazil.
authors: Larissa T
authors: LT
authors: Alvarim
authors: Hospital Israelita Albert Einstein, São Paulo, Brazil.
authors: Paulo H
authors: PH
authors: Silveira
authors: Hospital Israelita Albert Einstein, São Paulo, Brazil.
authors: Luciana C
authors: LC
authors: Marti
authors: Hospital Israelita Albert Einstein, São Paulo, Brazil ; Departamento de Neurologia e Neurociências, Universidade Federal de São Paulo, São Paulo, Brazil ; Faculdade de Ciências Médicas da Santa Casa de São Paulo, São Paulo, Brazil.
authors: Lf
authors: L
authors: Gamarra
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002465:Cell Movement; D058948:Cell Tracking / Q000379:methods*; D036101:Cord Blood Stem Cell Transplantation; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005456:Fluorescent Dyes; D006801:Humans; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D050997:Nanomedicine; D019636:Neurodegenerative Diseases / Q000473:pathology / Q000628:therapy; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D012235:Rhodamines; D013378:Substantia Nigra / Q000166:cytology
chemical_list: D005456:Fluorescent Dyes; D058185:Magnetite Nanoparticles; D012235:Rhodamines; C029773:rhodamine B
keywords: Parkinson’s disease; Rhodamine; magnetic resonance imaging; mesenchymal stem cells; multimodal iron oxide nanoparticles