title: Tim-3 on peripheral CD4⁺ and CD8⁺ T cells is involved in the development of glioma.
abstract: Tim-3 acts as a negative regulatory molecule and plays a critical role in immune tolerance. The purpose of this study was to investigate the expression of Tim-3 on peripheral CD4⁺ and CD8⁺ T cells in glioma. A total of 30 newly diagnosed glioma patients and 30 healthy controls were recruited and leukocytes from peripheral blood mononuclear cells were analyzed for Tim-3 surface expression by flow cytometry. Plasma tumor necrosis factor-alpha (TNF-α) was also measured. Data showed that expression of Tim-3 was significantly increased in both CD4⁺ and CD8⁺ T cells in glioma patients than in controls (p<0.001 and p<0.001, respectively). Patients with a higher tumor grade revealed further elevated Tim-3 expression in CD8⁺ T cells compared with those with a lower tumor grade. Also, the Karnofsky score of patients was negatively correlated with the percentage of Tim-3⁺CD8⁺ T cells in glioma patients (p=0.007). In addition, an inverse correlation was observed between the plasma level of TNF-α and Tim-3⁺CD4⁺ T cells (p=0.005) or Tim-3⁺CD8⁺ T cells (p<0.001) in glioma patients. Our results suggested that Tim-3 may be involved in the development of glioma.
authors: 1 Department of Neurosurgery, Daping Hospital, Third Military Medical University , Chongqing, China .
authors: Song
authors: S
authors: Han
authors: Sizhe
authors: S
authors: Feng
authors: Lunshan
authors: L
authors: Xu
authors: Weiwei
authors: W
authors: Shi
authors: Xuhui
authors: X
authors: Wang
authors: Hao
authors: H
authors: Wang
authors: Chunyong
authors: C
authors: Yu
authors: Tao
authors: T
authors: Dong
authors: Minhui
authors: M
authors: Xu
authors: Guobiao
authors: G
authors: Liang
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism*; D002681:China; D005434:Flow Cytometry; D005910:Glioma / Q000235:genetics* / Q000276:immunology* / Q000503:physiopathology; D000072597:Hepatitis A Virus Cellular Receptor 2; D006801:Humans; D007108:Immune Tolerance / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D018709:Statistics, Nonparametric; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: C448908:HAVCR2 protein, human; D000072597:Hepatitis A Virus Cellular Receptor 2; D008565:Membrane Proteins; D014409:Tumor Necrosis Factor-alpha