title: Determination of breath isoprene and acetone concentration with a needle-type extraction device in gas chromatography-mass spectrometry.
abstract: BACKGROUND
  Isoprene in human breath is said to be related to cholesterol metabolism, and the possibility of the correlations with some clinical parameters has been studied. However, at this stage, no clear benefit of breath isoprene has been reported for clinical diagnosis. In this work, isoprene and acetone concentrations were measured in the breath of healthy and obese subjects using a needle-type extraction device for subsequent analysis in gas chromatography-mass spectrometry (GC-MS) to investigate the possibility of these compounds as an indicator of possible diseases.
  
  
  METHODS
  After measuring intraday and interday variations of isoprene and acetone concentrations in breath samples of healthy subjects, their concentrations were also determined in 80 healthy and 17 obese subjects. In addition, correlation between these breath concentrations and the blood tests result was studied for these healthy and obese subjects.
  
  
  RESULTS
  The results indicated successful determination of breath isoprene and acetone in this work, however, no clear correlation was observed between these measured values and the blood test results.
  
  
  CONCLUSIONS
  Breath isoprene concentration may not be a useful indicator for obesity or hypercholesterolemia.
authors: Department of Applied Chemistry, University of Yamanashi, 4-3-11 Takeda, Kofu 400-8511, Japan.
authors: Ikuo
authors: I
authors: Ueta
authors: Department of Applied Chemistry, University of Yamanashi, 4-3-11 Takeda, Kofu 400-8511, Japan.
authors: Ayako
authors: A
authors: Mizuguchi
authors: Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tempakuku, Nagoya 468-8503, Japan.
authors: Mitsuyoshi
authors: M
authors: Okamoto
authors: Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tempakuku, Nagoya 468-8503, Japan.
authors: Hiroyuki
authors: H
authors: Sakamaki
authors: Ena Hospital, 2725 Oicho, Ena 509-7201, Japan.
authors: Masahiko
authors: M
authors: Hosoe
authors: Ishiguro Clinic, 6-37 Masakikitamachi, Gifu 502-0881, Japan.
authors: Motoyuki
authors: M
authors: Ishiguro
authors: Department of Environmental and Life Sciences, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempakucho, Toyohashi 441-8580, Japan. Electronic address: saito@ens.tut.ac.jp.
authors: Yoshihiro
authors: Y
authors: Saito
mesh_terms: D000096:Acetone / Q000032:analysis*; D000328:Adult; D000368:Aged; D001944:Breath Tests; D002070:Butadienes / Q000032:analysis*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D045782:Hemiterpenes / Q000032:analysis*; D006801:Humans; D006937:Hypercholesterolemia / Q000175:diagnosis; D008297:Male; D008875:Middle Aged; D009339:Needles; D009765:Obesity / Q000175:diagnosis; D010420:Pentanes / Q000032:analysis*
chemical_list: D002070:Butadienes; D045782:Hemiterpenes; D010420:Pentanes; C005059:isoprene; D000096:Acetone
keywords: Acetone; Breath analysis; Gas chromatography; Isoprene; Needle extraction