title: Pathway-level acceleration of glycogen catabolism by a response regulator in the cyanobacterium Synechocystis species PCC 6803.
abstract: Response regulators of two-component systems play pivotal roles in the transcriptional regulation of responses to environmental signals in bacteria. Rre37, an OmpR-type response regulator, is induced by nitrogen depletion in the unicellular cyanobacterium Synechocystis species PCC 6803. Microarray and quantitative real-time polymerase chain reaction analyses revealed that genes related to sugar catabolism and nitrogen metabolism were up-regulated by rre37 overexpression. Protein levels of GlgP(slr1367), one of the two glycogen phosphorylases, in the rre37-overexpressing strain were higher than those of the parental wild-type strain under both nitrogen-replete and nitrogen-depleted conditions. Glycogen amounts decreased to less than one-tenth by rre37 overexpression under nitrogen-replete conditions. Metabolome analysis revealed that metabolites of the sugar catabolic pathway and amino acids were altered in the rre37-overexpressing strain after nitrogen depletion. These results demonstrate that Rre37 is a pathway-level regulator that activates the metabolic flow from glycogen to polyhydroxybutyrate and the hybrid tricarboxylic acid and ornithine cycle, unraveling the mechanism of the transcriptional regulation of primary metabolism in this unicellular cyanobacterium.
authors: RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
authors: Takashi
authors: T
authors: Osanai
authors: Akira
authors: A
authors: Oikawa
authors: Keiji
authors: K
authors: Numata
authors: Ayuko
authors: A
authors: Kuwahara
authors: Hiroko
authors: H
authors: Iijima
authors: Yoshiharu
authors: Y
authors: Doi
authors: Kazuki
authors: K
authors: Saito
authors: Masami Yokota
authors: MY
authors: Hirai
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D002087:Butyrates / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial; D005947:Glucose / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism*; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D008954:Models, Biological; D009584:Nitrogen / Q000172:deficiency; D020411:Oligonucleotide Array Sequence Analysis; D010957:Plasmids / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D046939:Synechocystis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D002087:Butyrates; D002244:Carbon; D006003:Glycogen; D005978:Glutathione; D005947:Glucose; D009584:Nitrogen