title: Molecular analysis of OXA-48-carrying conjugative IncL/M-like plasmids in clinical isolates of Klebsiella pneumoniae in Ireland.
abstract: This study characterized an IncL/M-like plasmid containing a bla(OXA-48)-encoding gene from a clinical isolate of Klebsiella pneumoniae, denoted as E71T. Investigation of this plasmid sequence identified unique regions of interest along with conserved regions detected in eight other clinical carbapenem-resistant isolates. A 63-kb plasmid (pE71T) from K. pneumoniae E71T was sequenced and found to be highly similar to the recently published K. pneumoniae pOXA-48a (JN626286). Two copies of the insertion sequence element IS1R were identified, one of which was located adjacent to the bla(OXA-48)-encoding gene forming part of a composite transposon Tn1999.2 and the second located 16-kb downstream. Plasmid profiling and PCR assays confirmed that the pE71T backbone was conserved among the eight other clinical bla(OXA-48)-positive isolates, and in all cases, the OXA-48 genes were part of the Tn1999.2 composite transposon. This is the first report of a bla(OXA-48) and IS1R arrangement-containing plasmid in Ireland.
authors: 1 UCD Centre for Molecular Innovation & Drug Discovery, School of Public Health, Physiotherapy & Population Science, University College Dublin , Dublin, Ireland .
authors: Karen
authors: K
authors: Power
authors: Juan
authors: J
authors: Wang
authors: Maria
authors: M
authors: Karczmarczyk
authors: Brendan
authors: B
authors: Crowley
authors: Meaghan
authors: M
authors: Cotter
authors: Pippa
authors: P
authors: Haughton
authors: Maureen
authors: M
authors: Lynch
authors: Kirsten
authors: K
authors: Schaffer
authors: Séamus
authors: S
authors: Fanning
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000627:therapeutic use; D001483:Base Sequence; D015780:Carbapenems / Q000378:metabolism / Q000627:therapeutic use; D017124:Conserved Sequence; D004251:DNA Transposable Elements; D015870:Gene Expression; D006801:Humans; D007494:Ireland; D007710:Klebsiella Infections / Q000188:drug therapy / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010957:Plasmids / Q000737:chemistry*; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; D004251:DNA Transposable Elements; D001618:beta-Lactamases; C108449:oxacillinase