title: Theoretical and experimental studies on activity of Yarrowia lipolytica lipase in methanol/water mixtures.
abstract: Methanol is regularly used as a substrate for biodiesel synthesis. Excess methanol can however inhibit the lipase activity. In the present work, the activity and conformational changes of Yarrowia lipolytica lipase (LIP2) at different concentrations of methanol were investigated by measuring fluorescence, UV-vis spectra, and molecular dynamics simulations. The lipase tended to expand at higher concentrations of methanol, and the methanol molecules were able to enter into the LIP2, leading to microenvironment changes around the aromatic amino acids. More hydrophobic groups were exposed to the solvents at high methanol concentrations, and the original hydrophobic interaction in the protein was destroyed, thus resulting in the tertiary structure change of the lipase.
authors: Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology , Beijing 100029, China.
authors: Lingli
authors: L
authors: Li
authors: Yang
authors: Y
authors: Jiang
authors: Haiyang
authors: H
authors: Zhang
authors: Wei
authors: W
authors: Feng
authors: Biqiang
authors: B
authors: Chen
authors: Tianwei
authors: T
authors: Tan
mesh_terms: D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D008049:Lipase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D000432:Methanol / Q000737:chemistry* / Q000494:pharmacology*; D056004:Molecular Dynamics Simulation; D011487:Protein Conformation; D014867:Water / Q000737:chemistry*; D025062:Yarrowia / Q000201:enzymology*
chemical_list: D004791:Enzyme Inhibitors; D014867:Water; D008049:Lipase; D000432:Methanol