title: Effect of ambient light exposure of media and embryos on development and quality of porcine parthenogenetically activated embryos.
abstract: Light exposure is a common stress factor during in vitro handling of oocytes and embryos that originates from both microscope and ambient light. In the current study, the effect of two types of ambient light (daylight and laboratory light) on porcine parthenogenetically activated (PA) embryos was tested in two experiments: (1) ambient light on medium subsequently used for embryo in vitro development; and (2) ambient light exposure on activated oocytes before in vitro development. The results from Experiment 1 showed that exposure of culture medium to both types of ambient light decreased the percentage of blastocysts that showed good morphology, only after 24 h exposure. The results from Experiment 2 revealed a reduction in both blastocyst formation and quality when activated oocytes were exposed to both types of ambient light. This effect was seen after only 1 h exposure and increased with time. In conclusion, exposure to ambient light can be harmful to embryo development, both when medium is exposed for a long period of time and, to a greater extent, when the embryo itself is exposed for >1 h. In practice, it is therefore recommended to protect both culture medium and porcine embryos against ambient light during in vitro handling in the laboratory.
authors: Department of Animal Science,Aarhus University,DK-8830 Tjele,Denmark.
authors: Rong
authors: R
authors: Li
authors: Department of Animal Science,Aarhus University,DK-8830 Tjele,Denmark.
authors: Ying
authors: Y
authors: Liu
authors: Department of Animal Science,Aarhus University,DK-8830 Tjele,Denmark.
authors: Hanne Skovsgaard
authors: HS
authors: Pedersen
authors: Department of Animal Science,Aarhus University,DK-8830 Tjele,Denmark.
authors: Henrik
authors: H
authors: Callesen
mesh_terms: D000818:Animals; D017209:Apoptosis; D001755:Blastocyst / Q000502:physiology*; D003470:Culture Media / Q000494:pharmacology; D046149:Embryo Culture Techniques / Q000379:methods*; D005260:Female; D059471:In Vitro Oocyte Maturation Techniques; D008027:Light; D009865:Oocytes / Q000502:physiology*; D010312:Parthenogenesis; D034421:Sus scrofa
chemical_list: D003470:Culture Media
keywords: Pig