title: [High-fat diet induces pulmonary fibrosis in rats and inhibitory effects of N-acetylcysteine].
abstract: OBJECTIVE
  To explore the relation between high-fat diet and pulmonary fibrosis and the inhibition of N-acetylcysteine (NAC) on lung tissue in rats.
  
  
  METHODS
  Thirty Sprague Dawley (SD) male rats were randomly divided into control group (n = 10) on a quantitative control Lieber-DeCarli liquid diet, a high-fat diet group (n = 10) on a high fat-diet Lieber-DeCarli liquid diet and NAC group (n = 10) on NAC 300 mg×kg(-1)×d(-1). All rats were sacrificed 8 weeks later. The morphological changes and collagen deposition in lung tissue were observed by hematoxylin & eosin and Masson staining while the contents of glutathione (GSH) and hyaluronic acid (HA) measured through colorimetry and enzyme-linked immunosorbent assay. And the expression of transforming growth factor-β1 (TGF-β1) expression in lung tissue was detected through immunohistochemistry.
  
  
  RESULTS
  There were variable degree of alveolar and alveolar septal infiltration of inflammatory cells. And more deposition of collagen fibers appeared at intervals of alveolar in high fat group. Similar pathological changes were found in NAC group, but the degree was lower than that of high-fat group. Compared to the control and NAC groups, the lung tissue content of GSH decreased (GSH: 0.11 ± 0.05 vs 0.19 ± 0.11, 0.22 ± 0.14 mg/g, both P < 0.05) while HA and TGF-β1 increased in high-fat diet group (HA: 0.57 ± 0.06 vs 0.46 ± 0.04, 0.41 ± 0.04 mg/g; TGF-β1: 24.6 ± 3.4 vs 16.8 ± 2.6, 11.7 ± 1.5, all P < 0.05).
  
  
  CONCLUSIONS
  High-fat diet may induce pulmonary fibrosis in rats and NAC has inhibitory effects.
authors: Department of Respiratory Medicine, Tianjin Haihe Hospital, Institute of Respiratory Medicine Tianjin, Tianjin 300350, China.
authors: Hong-zhi
authors: HZ
authors: Yu
authors: Department of Respiratory Medicine, Tianjin Haihe Hospital, Institute of Respiratory Medicine Tianjin, Tianjin 300350, China. Email: wq572004@163.com.
authors: Qi
authors: Q
authors: Wu
authors: Department of Respiratory Medicine, Tianjin Haihe Hospital, Institute of Respiratory Medicine Tianjin, Tianjin 300350, China.
authors: Zhong-zhen
authors: ZZ
authors: Du
authors: Department of Respiratory Medicine, Tianjin Haihe Hospital, Institute of Respiratory Medicine Tianjin, Tianjin 300350, China.
authors: Xin
authors: X
authors: Sun
authors: Department of Respiratory Medicine, Tianjin Haihe Hospital, Institute of Respiratory Medicine Tianjin, Tianjin 300350, China.
authors: Cheng-zhi
authors: CZ
authors: Wang
authors: Department of Respiratory Medicine, Tianjin Haihe Hospital, Institute of Respiratory Medicine Tianjin, Tianjin 300350, China.
authors: Li
authors: L
authors: Li
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D059305:Diet, High-Fat / Q000009:adverse effects*; D004195:Disease Models, Animal; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D011658:Pulmonary Fibrosis / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D053773:Transforming Growth Factor beta1; D000111:Acetylcysteine