title: [Isolation of mouse primary hepatocytes by retrograde liver perfusion with catheterization via heart].
abstract: OBJECTIVE
  To explore the effects of retrograde liver perfusion with catheterization via heart on the isolation of primary mouse hepatocytes.
  
  
  METHODS
  In order to more efficiently isolate primary mouse hepatocytes, we improved the traditional two-step collagenase perfusion method. The liver perfusion catheter was inserted through right atrium and suprahepatic vena cava, and the perfusion velocity was controlled by the drip infusion with collagenase perfusate containing 10% of fetal calf serum.
  
  
  RESULTS
  Total success rate of catheterization in the improved group was as high as 95%, and the success rate of first at ttempt in the improved group was significantly higher than that in the traditional group (94.7% vs. 68.8%). Liver perfusion in the improved group was symmetrical with the high yield of hepatocytes up to 1.07 x 10(6) per gram of mouse weight and 92.16% of average cell vitality, which were higher than those in the traditional group.
  
  
  CONCLUSIONS
  The retrograde liver perfusion through the heart is a simple and easy-to-learn method to isolate mouse primary hepatocytes, which also could guarantee the satisfactory yield and vitality of primary hepatocytes.
authors: Department of Hepato-Biliary-Pancreatic Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Yan-Nan
authors: YN
authors: Bai
authors: Department of Hepato-Biliary-Pancreatic Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Tao
authors: T
authors: Zhang
authors: Department of Hepato-Biliary-Pancreatic Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Hong
authors: H
authors: Wu
authors: Department of Hepato-Biliary-Pancreatic Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Yong
authors: Y
authors: Zeng
mesh_terms: D000818:Animals; D002404:Catheterization; D002469:Cell Separation / Q000379:methods*; D017364:Collagenases; D006321:Heart; D022781:Hepatocytes / Q000166:cytology*; D008099:Liver; D051379:Mice; D010477:Perfusion
chemical_list: D017364:Collagenases