title: [Research progress of real-time quantitative PCR method for group A rotavirus detection].
abstract: Group A rotavirus is one of the most significant etiological agents which causes acute gastroenteritis among infants and young children worldwide. So far, several method which includes electron microscopy (EM), enzyme immunoassay (EIA), reverse transcription-polymerase chain reaction (RT-PCR)and Real-time Quantitative PCR has been established for the detection of rotavirus. Compared with other methods, Real-time quantitative PCR have advantages in specificity, sensitivity, genotyping and quantitative accuracy. This article shows a overview of the application of real-time quantitative PCR technique to detecte group A rotavirus.
authors: Department of Viral Diarrheal, Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China. qing9041@126.com
authors: Yan-Qing
authors: YQ
authors: Guo
authors: Department of Viral Diarrheal, Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.
authors: Dan-Di
authors: DD
authors: Li
authors: Department of Viral Diarrheal, Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.
authors: Zhao-Jun
authors: ZJ
authors: Duan
mesh_terms: D000818:Animals; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods / Q000639:trends*; D012401:Rotavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000175:diagnosis* / Q000821:virology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins