title: Human fecal source identification with real-time quantitative PCR.
abstract: Waterborne diseases represent a significant public health risk worldwide and can originate from contact with water contaminated with human fecal material. We describe a real-time quantitative PCR (qPCR) method that targets a genetic marker of the human-associated Bacteroides dorei for identification of human fecal pollution in ambient water samples. The following protocol includes water sample collection, filtration, DNA isolation with a sample processing control, qPCR amplification with an internal amplification control, and quality control data analysis.
authors: National Risk Management Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, Cincinnati, OH, USA.
authors: Orin C
authors: OC
authors: Shanks
authors: Lindsay
authors: L
authors: Peed
authors: Mano
authors: M
authors: Sivaganesan
authors: Richard A
authors: RA
authors: Haugland
authors: Eunice C
authors: EC
authors: Chern
mesh_terms: D005243:Feces / Q000382:microbiology*; D005819:Genetic Markers; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D014871:Water Microbiology; D014876:Water Pollution
chemical_list: D005819:Genetic Markers