title: Identification and functional characterization of heat shock transcription factor1 in Litopenaeus vannamei.
abstract: Heat shock transcription factors belong to the heat shock factor (HSF) protein family, which are involved in heat shock protein (HSP) gene regulation. They are critical for cell survival upon exposure to harmful conditions. In this study, we identified and characterized a HSF1 (LvHSF1) gene in Litopenaeus vannamei, with a full-length cDNA of 2841 bp and an open reading frame encoding a putative protein of 632 amino acids. Through multiple sequence alignment and phylogenetic analysis, it was revealed that LvHSF1 was closed to insect HSF family, which contained a highly conserved DNA-binding domain, oligomerization domains with HR-A/B, and a nuclear localization signal. Tissues distribution showed that LvHSF1 was widely expressed in all tissues tested. And it was upregulated in hemocytes and gills after Vibrio alginolyticus or Staphylococcus aureus infection. Dual-luciferase reporter assays indicated that LvHSF1 activated the promoters of L. vannamei HSP70 (LvHSP70) and L. vannamei Cactus (LvCactus), while inhibited the expressions of Drosophila antimicrobial peptide (AMP) Atta, Mtk, and L. vannamei AMP PEN4 through NF-κB signal transduction pathway modification. Knocked-down expression of LvHSF1 by dsRNA resulted in downregulations of LvHSP70 and LvCactus, as well as cumulative mortality decreasing under V. alginolyticus or S. aureus infection in L. vannamei. Taken together, our data strongly suggest that LvHSF1 is involved in LvHSP70 regulation, therefore plays a great role in stress resistance. And it also takes part in LvCactus/LvDorsal feedback regulatory pathway modification of L. vannamei, which is in favor of V. alginolyticus or S. aureus infection.
authors: MOE Key Laboratory of Aquatic Product Safety, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, People's Republic of China.
authors: Hui
authors: H
authors: Yan
authors: MOE Key Laboratory of Aquatic Product Safety, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, People's Republic of China.
authors: Shuang
authors: S
authors: Zhang
authors: MOE Key Laboratory of Aquatic Product Safety, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, People's Republic of China.
authors: Xiao-Yun
authors: XY
authors: Li
authors: MOE Key Laboratory of Aquatic Product Safety, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, People's Republic of China.
authors: Feng-Hua
authors: FH
authors: Yuan
authors: MOE Key Laboratory of Aquatic Product Safety, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, People's Republic of China.
authors: Wei
authors: W
authors: Qiu
authors: MOE Key Laboratory of Aquatic Product Safety, State Key Laboratory of Biocontrol, School of Marine Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, People's Republic of China.
authors: Yong-Gui
authors: YG
authors: Chen
authors: MOE Key Laboratory of Aquatic Product Safety, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, People's Republic of China.
authors: Shao-Ping
authors: SP
authors: Weng
authors: MOE Key Laboratory of Aquatic Product Safety, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, People's Republic of China; MOE Key Laboratory of Aquatic Product Safety, State Key Laboratory of Biocontrol, School of Marine Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, People's Republic of China. Electronic address: lsshjg@mail.sysu.edu.cn.
authors: Jian-Guo
authors: JG
authors: He
authors: MOE Key Laboratory of Aquatic Product Safety, State Key Laboratory of Biocontrol, School of Marine Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, People's Republic of China. Electronic address: chenyihong365@gmail.com.
authors: Yi-Hong
authors: YH
authors: Chen
mesh_terms: D000818:Animals; D001419:Bacteria / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D005880:Gills / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D000076249:Heat Shock Transcription Factors; D006434:Hemocytes / Q000378:metabolism; D008156:Luciferases; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D033561:Penaeidae / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D016415:Sequence Alignment; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D018840:HSP70 Heat-Shock Proteins; D000076249:Heat Shock Transcription Factors; D014157:Transcription Factors; D008156:Luciferases
keywords: Antimicrobial peptide genes; Cactus; Heat shock factor1; Litopenaeus vannamei; Nuclear factor-kappa B