title: Effect of the RW-Cb and its active ingredient like P-acid and P-aldehyde on primary rat osteoblasts.
abstract: BACKGROUND
  RW-Cb, the first processed product of Cibotium barometz (L.) J. Sm. (Dicksoniaceae) in TCM has been widely used to treat osteoporosis, a major worldwide health problem, influencing more and more people in the word et al. To do research on RW-Cb, one processed product of Cibotium barometz (L.) J. Sm. (Dicksoniaceae) in TCM has been widely used to treat osteoporosis, a major worldwide health problem, influencing more and more people in the word et al.
  
  
  METHODS
  The raw rhizome slices of Cibotium barometz were identified and both P-acid and P-aldehyde had a high purity. Primary rat osteoblasts were cultured and identified to be used for the assay.
  
  
  RESULTS
  RW-Cb contributed the most to osteoblasts proliferation and the AKP activity among all the groups; osteoblasts proliferation effect of P-acid and P-aldehyde: the mixed group(high>middle>low)>the P-acid group (high>middle>low) or the P-aldehyde group(high>middle>low), P<0.001; AKP activity: the P-acid's high group was higher than P-aldehyde's high, the mixed of low and middle groups had no significant difference, the rest of groups' results were similar to the osteoblasts' proliferation, P<0.05.
  
  
  CONCLUSIONS
  RW-Cb and its index constituents like P-acid and P-aldehyde could promote proliferation and differentiation of primary rat osteoblasts cultured in vitro, and it could be the theoretical basis for the treatment of osteoporosis.
authors: Gang
authors: G
authors: Xu
authors: Min-Jie
authors: MJ
authors: Zhao
authors: Na
authors: N
authors: Sun
authors: Cheng-Guo
authors: CG
authors: Ju
authors: Tian-Zhu
authors: TZ
authors: Jia
mesh_terms: D000447:Aldehydes / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D029624:Ferns / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal; D051381:Rats
chemical_list: D000447:Aldehydes; D010936:Plant Extracts