title: Identification and characterization of the Populus sucrose synthase gene family.
abstract: In this study, we indentified 15 sucrose synthase (SS) genes in Populus and the results of RT-qPCR revealed that their expression patterns were constitutive and partially overlapping but diverse. The release of the most recent Populus genomic data in Phytozome v9.1 has revealed the largest SS gene family described to date, comprising 15 distinct members. This information will now enable the analysis of transcript expression profiles for those that have not been previously reported. Here, we performed a comprehensive analysis of SS genes in Populus by describing the gene structure, chromosomal location and phylogenetic relationship of each family member. A total of 15 putative SS gene members were identified in the Populus trichocarpa (Torr. & Gray) genome using the SS domain and amino acid sequences from Arabidopsis thaliana as a probe. A phylogenetic analysis indicated that the 15 members could be classified into four groups that fall into three major categories: dicots, monocots & dicots 1 (M & D 1), and monocots & dicots 2 (M & D 2). In addition, the 15 SS genes were found to be unevenly distributed on seven chromosomes. The two conserved domains (sucrose synthase and glycosyl transferase) were found in this family. Meanwhile, the expression profiles of all 15 gene members in seven different organs were investigated in Populus tomentosa (Carr.) by using RT-qPCR. Additional analysis indicated that the poplar SS gene family is also involved in response to water-deficit. The current study provides basic information that will assist in elucidating the functions of poplar SS family.
authors: National Engineering Laboratory for Tree Breeding, NDRC, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, MOE, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory, SFA, College of Biological Sciences and Biotechnology, Beijing Forestry University, Qinghua East Road No.35, Haidian District, Beijing 100083, China. Electronic address: anxinmin@bjfu.edu.cn.
authors: Xinmin
authors: X
authors: An
authors: National Engineering Laboratory for Tree Breeding, NDRC, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, MOE, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory, SFA, College of Biological Sciences and Biotechnology, Beijing Forestry University, Qinghua East Road No.35, Haidian District, Beijing 100083, China.
authors: Zhong
authors: Z
authors: Chen
authors: National Engineering Laboratory for Tree Breeding, NDRC, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, MOE, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory, SFA, College of Biological Sciences and Biotechnology, Beijing Forestry University, Qinghua East Road No.35, Haidian District, Beijing 100083, China; Chinese Academy for Environmental Planning, Ministry of Environmental Protection of the People's Republic of China, Beijing 100012, China.
authors: Jingcheng
authors: J
authors: Wang
authors: National Engineering Laboratory for Tree Breeding, NDRC, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, MOE, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory, SFA, College of Biological Sciences and Biotechnology, Beijing Forestry University, Qinghua East Road No.35, Haidian District, Beijing 100083, China.
authors: Meixia
authors: M
authors: Ye
authors: National Engineering Laboratory for Tree Breeding, NDRC, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, MOE, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory, SFA, College of Biological Sciences and Biotechnology, Beijing Forestry University, Qinghua East Road No.35, Haidian District, Beijing 100083, China.
authors: Lexiang
authors: L
authors: Ji
authors: National Engineering Laboratory for Tree Breeding, NDRC, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, MOE, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory, SFA, College of Biological Sciences and Biotechnology, Beijing Forestry University, Qinghua East Road No.35, Haidian District, Beijing 100083, China.
authors: Jia
authors: J
authors: Wang
authors: National Engineering Laboratory for Tree Breeding, NDRC, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, MOE, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory, SFA, College of Biological Sciences and Biotechnology, Beijing Forestry University, Qinghua East Road No.35, Haidian District, Beijing 100083, China.
authors: Weihua
authors: W
authors: Liao
authors: National Engineering Laboratory for Tree Breeding, NDRC, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, MOE, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory, SFA, College of Biological Sciences and Biotechnology, Beijing Forestry University, Qinghua East Road No.35, Haidian District, Beijing 100083, China.
authors: Huandi
authors: H
authors: Ma
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D002874:Chromosome Mapping; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018745:Genome, Plant; D005964:Glucosyltransferases / Q000235:genetics*; D016695:Glycosyltransferases / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D032107:Populus / Q000235:genetics*; D016415:Sequence Alignment; D013395:Sucrose / Q000737:chemistry / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D013395:Sucrose; D016695:Glycosyltransferases; D005964:Glucosyltransferases; C022699:sucrose synthase
keywords: Expression profiles; Populus; RT-qPCR; Sucrose synthase; Water-deficit