title: Effect of intermittent aeration cycle on nutrient removal and microbial community in a fluidized bed reactor-membrane bioreactor combo system.
abstract: Effect of intermittent aeration cycle (IAC=15/45-60/60min) on nutrient removal and microbial community structure was investigated using a novel fluidized bed reactor-membrane bioreactor (FBR-MBR) combo system. FBR alone was found more efficient for removing PO4-P (>85%) than NH4-N (<40%) and chemical oxygen demand (COD<35%). However, in the combo system, COD and NH4-N removals were almost complete (>98%). Efficient nitrification, stable mixed liquor suspended solid and reduced transmembrane pressure was also achieved. Quantitative real-time polymerase chain reaction results of total bacteria 16S rRNA gene copies per mL of mixed-liquor varied from (2.48±0.42)×10(9) initial to (2.74±0.10)×10(8), (6.27±0.16)×10(9) and (9.17±1.78)×10(9) for 15/45, 45/15 and 60/60min of IACs, respectively. The results of clone library analysis revealed that Proteobacteria (59%), Firmicutes (12%) and Bacteroidetes (11%) were the dominant bacterial group in all samples. Overall, the combo system performs optimum nutrient removal and host stable microbial communities at 45/15min of IAC.
authors: UNEP-Tongji Institute of Environment for Sustainable Development, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
authors: Awoke
authors: A
authors: Guadie
authors: State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China. Electronic address: siqingxia@gmail.com.
authors: Siqing
authors: S
authors: Xia
authors: State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
authors: Zhiqiang
authors: Z
authors: Zhang
authors: Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China.
authors: Jemaneh
authors: J
authors: Zeleke
authors: Center for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Sydney, NSW 2007, Australia.
authors: Wenshan
authors: W
authors: Guo
authors: Center for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Sydney, NSW 2007, Australia.
authors: Huu Hao
authors: HH
authors: Ngo
authors: Department of Civil and Environmental Engineering, University of California, Berkeley, CA 94720, United States.
authors: Slawomir W
authors: SW
authors: Hermanowicz
mesh_terms: D000332:Aerobiosis; D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001673:Biodegradation, Environmental; D044822:Biodiversity; D057219:Biofouling; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology*; D005798:Genes, Bacterial; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000302:isolation & purification*; D010100:Oxygen / Q000032:analysis; D010758:Phosphorus / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S; D018508:Water Purification / Q000295:instrumentation* / Q000379:methods*
chemical_list: D008567:Membranes, Artificial; D012336:RNA, Ribosomal, 16S; D010758:Phosphorus; D009584:Nitrogen; D010100:Oxygen
keywords: Fluidized bed reactor; Intermittent aeration cycle; Membrane bioreactor; Microbial community; Nutrient removal