title: Multiwall carbon nanotube-ionic liquid electrode modified with gold nanoparticles as a base for preparation of a novel impedimetric immunosensor for low level detection of human serum albumin in biological fluids.
abstract: A highly sensitive ionic liquid-multiwall carbon nanotube based impedimetric immunosensor modified with gold nanoparticles (GNPs) was developed for the determination of human serum albumin (HSA). The antigen and antibody models used were HSA and activated anti-HSA, respectively. GNPs were electrodeposited on the multiwall based carbon ionic liquid electrode (MW-CILE) surface and then colloidal GNPs were coated through the thiol groups of 1,6-hexanedithiol (HDT) monolayer as a cross linker. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were employed for characterization of the various layers coated onto the electrode. The electron transfer resistance (Ret) of the antibody-modified electrode changed linearly with the concentration of HSA. The linear range and limit of detection were 0.1-100μgmL(-1) and 15.4ngmL(-1), respectively. The sensitivity and specificity of the immunosensor were validated using human urine and human serum samples. The results showed that the prepared immunosensor is a useful tool for screening trace amounts of HSA in the biological fluids of proteinuria and diabetics patients, in clinical laboratories.
authors: Nano Drug Delivery Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran; Department of Chemistry, Razi University, Kermanshah, Iran.
authors: Elham
authors: E
authors: Arkan
authors: Department of Medical Nanotechnology, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran; Research Center for Science and Technology in Medicine, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran.
authors: Reza
authors: R
authors: Saber
authors: Department of Chemistry, Razi University, Kermanshah, Iran.
authors: Ziba
authors: Z
authors: Karimi
authors: Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors: Ali
authors: A
authors: Mostafaie
authors: Department of Chemistry, Razi University, Kermanshah, Iran. Electronic address: mshamsipur@yahoo.com.
authors: Mojtaba
authors: M
authors: Shamsipur
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D052578:Ionic Liquids / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D052578:Ionic Liquids; D037742:Nanotubes, Carbon; D012709:Serum Albumin; D013438:Sulfhydryl Compounds; D006046:Gold; C501433:1,6-hexanedithiol
keywords: Carbon ionic liquid electrode; Electrochemical impedance spectroscopy; Gold nanoparticles; Immunosensor; Multiwall carbon nanotubes