title: Novel enhanced green fluorescent protein-expressing NOG mouse for analyzing the microenvironment of xenograft tissues.
abstract: The interaction between transplanted cells and host tissues is important for the growth and maintenance of transplanted cells. To analyze the mechanisms of these interactions, a systemic fluorescent protein-expressing mouse is a useful recipient. In this study, we generated a novel NOG strain, which strongly expresses enhanced green fluorescent protein (EGFP; PgkEGFP-NOG), especially in the liver, kidney, gastrointestinal tract, and testis. Because the host tissues expressed EGFP, xenotransplanted human cancer cells were clearly identified as EGFP-negative colonies in PgkEGFP-NOG mice. Immunohistochemical analysis revealed that EGFP-expressing stromal tissues formed a complicated tumor microenvironment within xenograft tissues. Moreover, a similar microenvironment was observed in human iPS cell-derived teratomas. Collectively, these results indicated that a suitable microenvironment is essential for the growth and maintenance of xenotransplanted cells and that PgkEGFP-NOG mice represent a useful animal model for analyzing the mechanisms of microenvironment formation.
authors: Yuichiro
authors: Y
authors: Higuchi
authors: Kenji
authors: K
authors: Kawai
authors: Masafumi
authors: M
authors: Yamamoto
authors: Miyuki
authors: M
authors: Kuronuma
authors: Yasuhiko
authors: Y
authors: Ando
authors: Ikumi
authors: I
authors: Katano
authors: Masato
authors: M
authors: Nakamura
authors: Hiroshi
authors: H
authors: Suemizu
mesh_terms: D000818:Animals; D015179:Colorectal Neoplasms / Q000473:pathology*; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000235:genetics*; D045325:HCT116 Cells; D064593:Heterografts; D006801:Humans; D007150:Immunohistochemistry; D057026:Induced Pluripotent Stem Cells / Q000473:pathology*; D051379:Mice; D016688:Mice, Inbred NOD; D023421:Models, Animal; D009368:Neoplasm Transplantation; D013724:Teratoma / Q000473:pathology*; D059016:Tumor Microenvironment
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins