title: A new derivative for oxosteroid analysis by mass spectrometry.
abstract: Here we report a new method for oxosteroid identification utilizing "tandem mass tag hydrazine" (TMTH) carbonyl-reactive derivatisation reagent. TMTH is a reagent with a chargeable tertiary amino group attached through a linker to a carbonyl-reactive hydrazine group. Thirty oxosteroids were analysed after derivatisation with TMTH by electrospray ionization mass spectrometry (ESI-MS) and were found to give high ion-currents compared to underivatised molecules. ESI-tandem mass spectrometry (MS/MS) analysis of the derivatives yielded characteristic fragmentation patterns with specific mass reporter ions derived from the TMT group. A shotgun ESI-MS method incorporating TMTH derivatisation was applied to a urine sample.
authors: College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK.
authors: K
authors: K
authors: Rigdova
authors: College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK.
authors: Y
authors: Y
authors: Wang
authors: Proteome Sciences plc, Coveham House, Downside Bridge Road, Cobham, Surrey KT11 3EP, UK.
authors: M
authors: M
authors: Ward
authors: College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK. Electronic address: w.j.griffiths@swansea.ac.uk.
authors: W J
authors: WJ
authors: Griffiths
mesh_terms: D000305:Adrenal Cortex Hormones / Q000652:urine; D000728:Androgens / Q000652:urine; D003687:Dehydroepiandrosterone / Q000737:chemistry / Q000652:urine; D013196:Dihydrotestosterone / Q000737:chemistry / Q000652:urine; D006801:Humans; D006834:Hydrazines / Q000737:chemistry*; D007664:Ketosteroids / Q000652:urine*; D009277:Nandrolone / Q000737:chemistry / Q000652:urine; D011372:Progestins / Q000652:urine; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods; D013739:Testosterone / Q000737:chemistry / Q000652:urine
chemical_list: D000305:Adrenal Cortex Hormones; D000728:Androgens; D006834:Hydrazines; D007664:Ketosteroids; D011372:Progestins; D013196:Dihydrotestosterone; C029424:hydrazine; D013739:Testosterone; D003687:Dehydroepiandrosterone; D009277:Nandrolone
keywords: Derivatisation; Steroid glucuronides; Steroids; Tandem mass spectrometry; Urine