title: Comprehensive assessment of phenolics and antiradical potential of Rumex hastatus D. Don. roots.
abstract: BACKGROUND
  Roots of Rumex hastatus (Polygonaceae) are traditionally used for the treatment of various ailments including liver and lung diseases. In this study, various solvent extracts of R. hastatus roots, like methanolic, n-hexane, ethyl acetate, chloroform, butanol and aqueous fractions were assessed through their antioxidant properties in vitro and determination of phenolic contents.
  
  
  METHODS
  Several parameters like DPPH˙, ABTS˙(+), ˙OH, H2O2, superoxide free radical scavenging, iron chelating power, reducing power, β-carotene bleaching power, antioxidant capacity and total phenolics and flavonoids were evaluated. High Performance liquid Chromatography (HPLC) was also considered.
  
  
  RESULTS
  Though all the fractions exhibited dose dependant activity. The samples with the highest activity were the butanol and methanol fractions in all the assays except hydrogen peroxide radical scavenging assay where chloroform fraction showed the highest scavenging aptitude. On the other hand, aquous fraction showed significant beta carotene linoleic acid, while n-hexane and ethyl acetate fractions exhibited a lesser antioxidant activity in all the assays. HPLC revealed the presence of rutin, luteolin-7-glucoside, vitexin and luteolin.
  
  
  CONCLUSIONS
  These results have to some extent substantiated the use of R. hastatus roots against different diseases, as an excellent basis of potential antioxidant due to the presence of sufficient amount of phenolics such as rutin and luteolin.
authors: Botanical Sciences Division, Pakistan Museum of Natural History, Garden Avenue, Shakarparian, Islamabad, Pakistan. sumairasahreen@gmail.com.
authors: Sumaira
authors: S
authors: Sahreen
authors: Muhammad Rashid
authors: MR
authors: Khan
authors: Rahmat Ali
authors: RA
authors: Khan
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D047310:Apigenin / Q000032:analysis / Q000494:pharmacology; D052160:Benzothiazoles / Q000378:metabolism; D001713:Biphenyl Compounds / Q000378:metabolism; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology*; D005960:Glucosides / Q000032:analysis / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism; D047311:Luteolin / Q000032:analysis / Q000494:pharmacology; D010636:Phenols / Q000032:analysis / Q000494:pharmacology*; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D031804:Rumex / Q000737:chemistry*; D012431:Rutin / Q000032:analysis / Q000494:pharmacology; D013451:Sulfonic Acids / Q000378:metabolism; D019207:beta Carotene / Q000032:analysis / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D005419:Flavonoids; D005960:Glucosides; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D013451:Sulfonic Acids; D019207:beta Carotene; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D012431:Rutin; D047310:Apigenin; C066408:luteolin-7-glucoside; C032731:vitexin; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D047311:Luteolin