title: Mitotic regulator Mis18β interacts with and specifies the centromeric assembly of molecular chaperone holliday junction recognition protein (HJURP).
abstract: The centromere is essential for precise and equal segregation of the parental genome into two daughter cells during mitosis. CENP-A is a unique histone H3 variant conserved in eukaryotic centromeres. The assembly of CENP-A to the centromere is mediated by Holliday junction recognition protein (HJURP) in early G1 phase. However, it remains elusive how HJURP governs CENP-A incorporation into the centromere. Here we show that human HJURP directly binds to Mis18β, a component of the Mis18 complex conserved in the eukaryotic kingdom. A minimal region of HJURP for Mis18β binding was mapped to residues 437-460. Depletion of Mis18β by RNA interference dramatically impaired HJURP recruitment to the centromere, indicating the importance of Mis18β in HJURP loading. Interestingly, phosphorylation of HJURP by CDK1 weakens its interaction with Mis18β, consistent with the notion that assembly of CENP-A to the centromere is achieved after mitosis. Taken together, these data define a novel molecular mechanism underlying the temporal regulation of CENP-A incorporation into the centromere by accurate Mis18β-HJURP interaction.
authors: From the Hefei National Laboratory of Physical Sciences at Microscale, University of Science and Technology of China School of Life Science, Hefei 230027, China.
authors: Jianyu
authors: J
authors: Wang
authors: Xing
authors: X
authors: Liu
authors: Zhen
authors: Z
authors: Dou
authors: Liang
authors: L
authors: Chen
authors: Hao
authors: H
authors: Jiang
authors: Chuanhai
authors: C
authors: Fu
authors: Guosheng
authors: G
authors: Fu
authors: Dan
authors: D
authors: Liu
authors: Jiancun
authors: J
authors: Zhang
authors: Tongge
authors: T
authors: Zhu
authors: Jingwen
authors: J
authors: Fang
authors: Jianye
authors: J
authors: Zang
authors: Jinke
authors: J
authors: Cheng
authors: Maikun
authors: M
authors: Teng
authors: Xia
authors: X
authors: Ding
authors: Xuebiao
authors: X
authors: Yao
mesh_terms: D018797:Cell Cycle Proteins; D002460:Cell Line; D002503:Centromere / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D008938:Mitosis; D010766:Phosphorylation; D011485:Protein Binding; D060066:Protein Interaction Maps
chemical_list: D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; C524371:HJURP protein, human; C517146:OIP5 protein, human
keywords: Cell Division; Centromeres; Chromatin Structure; Histone Chaperone; Phosphorylation