title: Cycloamylose production from amylomaize by isoamylase and Thermus aquaticus 4-α-glucanotransferase.
abstract: Amylomaize (AMM) was utilized as the substrate for cycloamylose (CA) production in this study. After debranching, AMM was incubated with 10 U/g of Thermus aquaticus 4-α-glucanotransferase (TA 4αGTase) for various reaction times in water or in DMSO reaction system. The maximum conversion yield of CA was greatly improved from 24.55% to 45.58% and from 27.40% to 47.25% after debranching in the water and DMSO reaction systems, respectively. Compared with the method that produced CA from commercial potato amylose, this method produced CA from a natural amylopectin containing starch with enhanced conversion yield after debranching. Meanwhile, we found that the minimum degree of polymerization (DP) of CA from TA 4αGTase treatment was 5, regardless of the reaction conditions. These results were different from those reported in the literature that stated the minimum DP of CA produced with a TA 4αGTase treatment was 22 regardless of the reaction conditions.
authors: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China. Electronic address: xuyan19890225@163.com.
authors: Yan
authors: Y
authors: Xu
authors: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China. Electronic address: zhouxing@jiangnan.edu.cn.
authors: Xing
authors: X
authors: Zhou
authors: Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands. Electronic address: y.bai@rug.nl.
authors: Yuxiang
authors: Y
authors: Bai
authors: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address: wangjinpeng@jiangnan.edu.cn.
authors: Jinpeng
authors: J
authors: Wang
authors: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address: wuchunsen@126.com.
authors: Chunsen
authors: C
authors: Wu
authors: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China. Electronic address: xmxu@jiangnan.edu.cn.
authors: Xueming
authors: X
authors: Xu
authors: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China. Electronic address: jinlab2008@yahoo.com.
authors: Zhengyu
authors: Z
authors: Jin
mesh_terms: D003505:Cyclodextrins / Q000096:biosynthesis*; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D006004:Glycogen Debranching Enzyme System / Q000378:metabolism*; D007517:Isoamylase / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013213:Starch / Q000378:metabolism*; D013824:Thermus / Q000201:enzymology*
chemical_list: D003505:Cyclodextrins; D006004:Glycogen Debranching Enzyme System; D013213:Starch; C020569:4 alpha-glucanotransferase; D007517:Isoamylase; D004121:Dimethyl Sulfoxide
keywords: Amylomaize; Cycloamylose; Isoamylase; Thermus aquaticus 4-α-glucanotransferase