title: Introducing samples directly into electrospray ionization mass spectrometers by direct infusion using a nanoelectrospray interface.
abstract: Procedures are described for constructing and using a microscale electrospray interface for direct infusion of samples into mass spectrometers. The sensitivity of the nanospray interface is a result of greatly reducing the flow of sample solution while preserving the analyte signal intensity. The described methodology provides a simple and robust way to analyze individual purified peptide and protein samples, i.e., samples that do not require liquid chromatography separation.
authors: Beckman Research Institute of the City of Hope, Duarte, California.
authors: Terry D
authors: TD
authors: Lee
authors: Beckman Research Institute of the City of Hope, Duarte, California.
authors: Roger E
authors: RE
authors: Moore
authors: Beckman Research Institute of the City of Hope, Duarte, California.
authors: Denise
authors: D
authors: Keen
mesh_terms: D036103:Nanotechnology / Q000295:instrumentation*; D010455:Peptides / Q000032:analysis; D011506:Proteins / Q000032:analysis; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods*
chemical_list: D010455:Peptides; D011506:Proteins
keywords: mass spectrometry; microscale electrospray interface; nanoelectrospray