title: Isocitrate dehydrogenase-1 mutations as prognostic biomarker in glioblastoma multiforme patients in West Bohemia.
abstract: BACKGROUND
  Glioblastoma multiforme (GBM) is the most malignant primary brain tumor in adults. Recent whole-genome studies revealed novel GBM prognostic biomarkers such as mutations in metabolic enzyme IDH-isocitrate dehydrogenases (IDH1 and IDH2). The distinctive mutation IDH1 R132H was uncovered to be a strong prognostic biomarker for glioma patients. We investigated the prognostic role of IDH1 R132H mutation in GBM patients in West Bohemia.
  
  
  METHODS
  The IDH1 R132H mutation was assessed by the RT-PCR in the tumor samples from 45 GBM patients treated in the Faculty Hospital in Pilsen and was correlated with the progression free and overall survival.
  
  
  RESULTS
  The IDH1 R132H mutation was identified in 20 from 44 GBM tumor samples (45.4%). The majority of mutated tumors were secondary GBMs (16 in 18, 89.9%). Low frequency of IDH1 mutations was observed in primary GBMs (4 in 26, 15.3%). Patients with IDH R132H mutation had longer PFS, 136 versus 51 days (P < 0.021, Wilcoxon), and OS, 270 versus 130 days (P < 0.024, Wilcoxon test).
  
  
  CONCLUSIONS
  The prognostic value of IDH1 R132H mutation in GBM patients was verified. Patients with mutation had significantly longer PFS and OS than patients with wild-type IDH1 and suffered more likely from secondary GBMs.
authors: Department of Neurology, Faculty of Medicine in Pilsen, Charles University in Prague, Husova 3, 301 66 Pilsen, Czech Republic ; Faculty Hospital in Pilsen, Alej Svobody 80, 304 60 Pilsen, Czech Republic.
authors: J
authors: J
authors: Polivka
authors: Department of Histology and Embryology and Biomedical Centre, Faculty of Medicine in Pilsen, Charles University in Prague, Husova 3, 301 66 Pilsen, Czech Republic.
authors: J
authors: J
authors: Polivka
authors: Department of Neurology, Faculty of Medicine in Pilsen, Charles University in Prague, Husova 3, 301 66 Pilsen, Czech Republic ; Faculty Hospital in Pilsen, Alej Svobody 80, 304 60 Pilsen, Czech Republic.
authors: V
authors: V
authors: Rohan
authors: Department of Biology, Faculty of Medicine in Pilsen, Charles University in Prague, Husova 3, 301 66 Pilsen, Czech Republic.
authors: M
authors: M
authors: Pesta
authors: Department of Neurology, Faculty of Medicine in Pilsen, Charles University in Prague, Husova 3, 301 66 Pilsen, Czech Republic ; Faculty Hospital in Pilsen, Alej Svobody 80, 304 60 Pilsen, Czech Republic.
authors: T
authors: T
authors: Repik
authors: Department of Histology and Embryology and Biomedical Centre, Faculty of Medicine in Pilsen, Charles University in Prague, Husova 3, 301 66 Pilsen, Czech Republic.
authors: P
authors: P
authors: Pitule
authors: Central Immunoanalytical Laboratory, Faculty Hospital in Pilsen, Dr. E. Benese 13, 305 99 Pilsen, Czech Republic.
authors: O
authors: O
authors: Topolcan
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D001932:Brain Neoplasms / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics* / Q000401:mortality; D018153:Czech Republic / Q000453:epidemiology; D005260:Female; D005909:Glioblastoma / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics* / Q000401:mortality; D006801:Humans; D007521:Isocitrate Dehydrogenase / Q000235:genetics*; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012189:Retrospective Studies
chemical_list: D014408:Biomarkers, Tumor; D007521:Isocitrate Dehydrogenase