title: Heat-shock protein A8 restores sperm membrane integrity by increasing plasma membrane fluidity.
abstract: The constitutive 70  kDa heat-shock protein, HSPA8, has previously been shown to contribute to the long-term survival of spermatozoa inside the mammalian female reproductive tract. Here, we show that a recombinant form of HSPA8 rapidly promotes the viability of uncapacitated spermatozoa, the ability of spermatozoa to bind to oviductal epithelial cells, enhances IVF performance, and decreases sperm mitochondrial activity. Fluorescence recovery after photobleaching revealed that the repair of membrane damage is achieved by an almost instantaneous increase in sperm membrane fluidity. The ability of HSPA8 to influence membrane stability and fluidity, as well as its conserved nature among mammalian species, supports the idea that this protein protects sperm survival through membrane repair mechanisms. Free Persian abstract A Persian translation of the abstract is freely available online at http://www.reproduction-online.org/content/147/5/719/suppl/DC1.
authors: Academic Unit of Reproductive and Developmental Medicine, Department of Human Metabolism, The Medical School, University of Sheffield, Sheffield, UK.
authors: Najmeh
authors: N
authors: Moein-Vaziri
authors: Ian
authors: I
authors: Phillips
authors: Simon
authors: S
authors: Smith
authors: Carmen
authors: C
authors: Almiňana
authors: Carolina
authors: C
authors: Maside
authors: Maria A
authors: MA
authors: Gil
authors: Jordi
authors: J
authors: Roca
authors: Emilio A
authors: EA
authors: Martinez
authors: William V
authors: WV
authors: Holt
authors: A Graham
authors: AG
authors: Pockley
authors: Alireza
authors: A
authors: Fazeli
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology; D002784:Cholesterol / Q000502:physiology; D018840:HSP70 Heat-Shock Proteins / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D008560:Membrane Fluidity / Q000502:physiology*; D023421:Models, Animal; D013094:Spermatozoa / Q000502:physiology*; D013552:Swine
chemical_list: D018840:HSP70 Heat-Shock Proteins; D002784:Cholesterol