title: Control of Cdc28 CDK1 by a stress-induced lncRNA.
abstract: Genomic analysis has revealed the existence of a large number of long noncoding RNAs (lncRNAs) with different functions in a variety of organisms, including yeast. Cells display dramatic changes of gene expression upon environmental changes. Upon osmostress, hundreds of stress-responsive genes are induced by the stress-activated protein kinase (SAPK) p38/Hog1. Using whole-genome tiling arrays, we found that Hog1 induces a set of lncRNAs upon stress. One of the genes expressing a Hog1-dependent lncRNA in antisense orientation is CDC28, the cyclin-dependent kinase 1 (CDK1) that controls the cell cycle in yeast. Cdc28 lncRNA mediates the establishment of gene looping and the relocalization of Hog1 and RSC from the 3' UTR to the +1 nucleosome to induce CDC28 expression. The increase in the levels of Cdc28 results in cells able to reenter the cell cycle more efficiently after stress. This may represent a general mechanism to prime expression of genes needed after stresses are alleviated.
authors: Cell Signaling Unit, Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra (UPF), 08003 Barcelona, Spain.
authors: Mariona
authors: M
authors: Nadal-Ribelles
authors: Cell Signaling Unit, Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra (UPF), 08003 Barcelona, Spain.
authors: Carme
authors: C
authors: Solé
authors: EMBL Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
authors: Zhenyu
authors: Z
authors: Xu
authors: EMBL Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
authors: Lars M
authors: LM
authors: Steinmetz
authors: Cell Signaling Unit, Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra (UPF), 08003 Barcelona, Spain. Electronic address: eulalia.nadal@upf.edu.
authors: Eulàlia
authors: E
authors: de Nadal
authors: Cell Signaling Unit, Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra (UPF), 08003 Barcelona, Spain. Electronic address: francesc.posas@upf.edu.
authors: Francesc
authors: F
authors: Posas
mesh_terms: D020413:3' Untranslated Regions; D017489:CDC28 Protein Kinase, S cerevisiae / Q000378:metabolism*; D002453:Cell Cycle; D047369:Chromatin Immunoprecipitation; D005434:Flow Cytometry; D015966:Gene Expression Regulation, Fungal; D049452:Green Fluorescent Proteins / Q000378:metabolism; D048055:Mitogen-Activated Protein Kinase 8 / Q000378:metabolism*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D009707:Nucleosomes / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000235:genetics; D009997:Osmotic Pressure; D011401:Promoter Regions, Genetic; D062085:RNA, Long Noncoding / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D020413:3' Untranslated Regions; D009707:Nucleosomes; D016376:Oligonucleotides, Antisense; D062085:RNA, Long Noncoding; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins; D017489:CDC28 Protein Kinase, S cerevisiae; C088589:HOG1 protein, S cerevisiae; D048055:Mitogen-Activated Protein Kinase 8; D020928:Mitogen-Activated Protein Kinases