title: Prolonged exposure to bradykinin and prostaglandin E2 increases TRPV1 mRNA but does not alter TRPV1 and TRPV1b protein expression in cultured rat primary sensory neurons.
abstract: Sensitisation of the capsaicin receptor, transient receptor potential vanilloid type 1 (TRPV1) ion channel in nociceptive primary sensory neurons (PSN) underlies the development of inflammatory heat hyperalgesia. Removal of the negative-dominant splice variant of the TRPV1 molecule, TRPV1b from TRPV1/TRPV1b heterotetrameric channels, which should be associated with changes in the expression of TRPV1 and TRPV1b transcripts and proteins, has been suggested to contribute to that sensitisation. Respective reverse-transcriptase polymerase chain reaction (RT-PCR) and Western-blotting revealed that both TRPV1 and TRPV1b mRNA, and their encoded proteins are expressed in rat cultured PSN. Sequencing of the RT-PCR products showed that TRPV1b mRNA lacks the entire exon 7. Further, growing PSN for 2 days in the presence of 10μM bradykinin (BK) and 10μM prostaglandin E2 (PGE2) significantly increases TRPV1 responsiveness and TRPV1 mRNA expression, without producing any changes in TRPV1b mRNA, and TRPV1 and TRPV1b protein expression. These data challenge the hypothesis that alterations in the composition of the TRPV1 ion channel contributes to the sensitisation.
authors: Section of Anaesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Imperial College London, Chelsea and Westminster Hospital, 369 Fulham Road. London, SW10 9NH, United Kingdom.
authors: Shilpa
authors: S
authors: Mistry
authors: Section of Anaesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Imperial College London, Chelsea and Westminster Hospital, 369 Fulham Road. London, SW10 9NH, United Kingdom.
authors: Cleoper C
authors: CC
authors: Paule
authors: Section of Anaesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Imperial College London, Chelsea and Westminster Hospital, 369 Fulham Road. London, SW10 9NH, United Kingdom.
authors: Angelika
authors: A
authors: Varga
authors: Division of Oncology, Department of Surgery and Cancer Imperial College London, Hammersmith Hospital, Du Cane Road, London, W12 0NN, United Kingdom.
authors: Andy
authors: A
authors: Photiou
authors: Section of Anaesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Imperial College London, Chelsea and Westminster Hospital, 369 Fulham Road. London, SW10 9NH, United Kingdom; Department of Physiology, University of Debrecen, Medical and Health Science Centre, Nagyerdei krt. 98, Debrecen, H-4012, Hungary.
authors: Agnes
authors: A
authors: Jenes
authors: Department of Experimental Biology, Faculty of Medicine, Alameda Prof. Hernani Monteiro, 4200-319, Porto, Portugal; IBMC, University of Porto, Rua do Campo Alegre, 823, 4150-180, Porto, Portugal.
authors: Antonio
authors: A
authors: Avelino
authors: Division of Oncology, Department of Surgery and Cancer Imperial College London, Hammersmith Hospital, Du Cane Road, London, W12 0NN, United Kingdom.
authors: Laki
authors: L
authors: Buluwela
authors: Section of Anaesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Imperial College London, Chelsea and Westminster Hospital, 369 Fulham Road. London, SW10 9NH, United Kingdom. Electronic address: i.nagy@imperial.ac.uk.
authors: Istvan
authors: I
authors: Nagy
mesh_terms: D000818:Animals; D001920:Bradykinin / Q000494:pharmacology*; D002211:Capsaicin / Q000494:pharmacology; D002478:Cells, Cultured; D015232:Dinoprostone / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000187:drug effects / Q000378:metabolism; D009619:Nociceptors / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D050916:TRPV Cation Channels / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D050916:TRPV Cation Channels; C493922:Trpv1 protein, rat; D015232:Dinoprostone; D002211:Capsaicin; D001920:Bradykinin
keywords: Capsaicin; Dorsal root ganglion; Inflammation; Pain