title: Development of an orange juice in-house reference material and its application to guarantee the quality of vitamin C determination in fruits, juices and fruit pulps.
abstract: Reference materials are useful for the quality control of analytical procedures and to evaluate the performance of laboratories. There are few and expensive certified reference materials commercially available for vitamin C or ascorbic acid analysis in food matrices. In this study, the preparation and the suitability assessment of an orange juice in-house reference material (RM) for vitamin C analysis in fruits, juices and in fruit pulps is described. This RM was used for the development and full validation of an HPLC method. The results showed excellent linearity (r(2)=0.9995), good accuracy (96.6-97.3%) and precision, as relative standard deviation, ranged from 0.70% to 3.67%. The in-house RM was homogenous and stable at storage conditions (-80°C) during 12 months. According to our results, this in-house RM is an excellent tool to use in quality control and method verification purposes for vitamin C analysis of fruits, juices and fruit pulps matrices. Furthermore, a stabilization solution with perchloric and metaphosphoric acids was developed which prevents degradation of ascorbic acid for a period of 12 months at -80°C.
authors: Department of Food and Nutrition, National Institute of Health Dr. Ricardo Jorge, I.P. (INSA), Av. Padre Cruz, 1649-016 Lisbon, Portugal.
authors: A
authors: A
authors: Valente
authors: Department of Food and Nutrition, National Institute of Health Dr. Ricardo Jorge, I.P. (INSA), Av. Padre Cruz, 1649-016 Lisbon, Portugal.
authors: A
authors: A
authors: Sanches-Silva
authors: Department of Food and Nutrition, National Institute of Health Dr. Ricardo Jorge, I.P. (INSA), Av. Padre Cruz, 1649-016 Lisbon, Portugal.
authors: T G
authors: TG
authors: Albuquerque
authors: Department of Food and Nutrition, National Institute of Health Dr. Ricardo Jorge, I.P. (INSA), Av. Padre Cruz, 1649-016 Lisbon, Portugal. Electronic address: helena.costa@insa.min-saude.pt.
authors: H S
authors: HS
authors: Costa
mesh_terms: D001205:Ascorbic Acid / Q000032:analysis*; D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D032084:Citrus sinensis / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D011786:Quality Control; D012015:Reference Standards; D014866:Waste Products / Q000032:analysis*
chemical_list: D014866:Waste Products; D001205:Ascorbic Acid
keywords: Ascorbic acid; Quality control; Reference material; Validation; Vitamin C