title: [Study on miR-490-5p and miR-363 as novel biomarkers for the diagnosis of colorectal cancer].
abstract: OBJECTIVE
  To identify dysregulated microRNA(miRNA) that can act as novel biomarker for colorectal cancer screening.
  
  
  METHODS
  Real time-polymerase chain reaction was used to detect the expression profile of miRNA in tumor and paired normal tissues, and the significant dysregulated miRNA was examined by non-paired t-test. Receiver operating characteristic(ROC) curve was performed to evaluate the specificity and sensitivity of miR-363 and miR-490-5p as biomarkers to discriminate colorectal cancer patients from normal person.
  
  
  RESULTS
  Seven-three dysregulated miRNAs were found in male samples, of which 6 miRNAs were up-regulated and other 67 miRNAs were down-regulated, while 42 dysregulated miRNAs were found in female samples, of which 5 were up-regulated and 37 were down-regulated in tumor tissues compared with normal tissues. Among above dysregulated miRNAs, 33 miRNAs had significant differences, besides, dysregulated expression level of 10 of 33 miRNAs was over 5 folds in male and female as well as mixed samples.
  
  
  CONCLUSIONS
  The expression pattern in female is different from that in male. miR-363 and miR-490-5p possess the potential in screening colorectal cancer patients from healthy people.
authors: Deparatment of General Surgery, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou 510150, China. maxtiger@126.com.
authors: Xuehu
authors: X
authors: Xu
authors: Xiaobing
authors: X
authors: Wu
authors: Shangbiao
authors: S
authors: Wu
authors: Qingping
authors: Q
authors: Jiang
authors: Haibo
authors: H
authors: Liu
authors: Rong
authors: R
authors: Chen
authors: Yan
authors: Y
authors: Sun
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000175:diagnosis*; D055088:Early Detection of Cancer; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000032:analysis*; D008875:Middle Aged; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; C574861:MIRN363 microRNA, human; C574862:MIRN490 microRNA, human; D035683:MicroRNAs