title: Expanding the potential of G-quadruplex structures: formation of a heterochiral TBA analogue.
abstract: In order to expand the potential applications of G-quadruplex structures, we explored the ability of heterochiral oligodeoxynucleotides based on the thrombin-binding aptamer (TBA) sequence to fold into similar complexes, with particular focus on their resistance in biological environments. A combination of CD and NMR techniques was used. Similarly to TBA, the ODN ggTTggtgtggTTgg (lower case letters indicate L residues) is able to fold into a chair-like antiparallel G-quadruplex structure, but has a slightly higher thermal stability. The discovery that heterochiral ODNs are able to form stable G-quadruplex structures opens up new possibilities for their development in several fields, as aptamers, sensors and, as recently shown, as catalysts for enantioselective reactions.
authors: Dipartimento di Farmacia, Università degli Studi di Napoli Federico II, Via D. Montesano 49, 80131 Napoli (Italy).
authors: Antonella
authors: A
authors: Virgilio
authors: Michela
authors: M
authors: Varra
authors: Maria
authors: M
authors: Scuotto
authors: Antonella
authors: A
authors: Capuozzo
authors: Carlo
authors: C
authors: Irace
authors: Luciano
authors: L
authors: Mayol
authors: Veronica
authors: V
authors: Esposito
authors: Aldo
authors: A
authors: Galeone
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D002942:Circular Dichroism; D054856:G-Quadruplexes; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D009838:Oligodeoxyribonucleotides; C081676:thrombin aptamer
keywords: G-quadruplexes; aptamers; biological activity; heterochiral oligonucleotides