title: A medium hyperglycosylated podocalyxin enables noninvasive and quantitative detection of tumorigenic human pluripotent stem cells.
abstract: While human pluripotent stem cells are attractive sources for cell-replacement therapies, a major concern remains regarding their tumorigenic potential. Thus, safety assessment of human pluripotent stem cell-based products in terms of tumorigenicity is critical. Previously we have identified a pluripotent stem cell-specific lectin probe rBC2LCN recognizing hyperglycosylated podocalyxin as a cell surface ligand. Here we demonstrate that hyperglycosylated podocalyxin is secreted from human pluripotent stem cells into cell culture supernatants. We establish a sandwich assay system, named the GlycoStem test, targeting the soluble hyperglycosylated podocalyxin using rBC2LCN. The GlycoStem test is sufficiently sensitive and quantitative to detect residual human pluripotent stem cells. This work provides a proof of concept for the noninvasive and quantitative detection of tumorigenic human pluripotent stem cells using cell culture supernatants. The developed method should increase the safety of human pluripotent stem cell-based cell therapies.
authors: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 2, 1-1-1 Umezono, Tsukuba, Ibaraki 305-8568, Japan.
authors: Hiroaki
authors: H
authors: Tateno
authors: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan.
authors: Yasuko
authors: Y
authors: Onuma
authors: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan.
authors: Yuzuru
authors: Y
authors: Ito
authors: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 2, 1-1-1 Umezono, Tsukuba, Ibaraki 305-8568, Japan.
authors: Keiko
authors: K
authors: Hiemori
authors: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan.
authors: Yasuhiko
authors: Y
authors: Aiki
authors: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan.
authors: Madoka
authors: M
authors: Shimizu
authors: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan.
authors: Kumiko
authors: K
authors: Higuchi
authors: Cell Biology Research Center, Life Science Research Laboratories, Wako Pure Chemical Industries, Ltd. 6-1 Takada-cho, Amagasaki, Hyogo 661-0963, Japan.
authors: Masakazu
authors: M
authors: Fukuda
authors: Cell Biology Research Center, Life Science Research Laboratories, Wako Pure Chemical Industries, Ltd. 6-1 Takada-cho, Amagasaki, Hyogo 661-0963, Japan.
authors: Masaki
authors: M
authors: Warashina
authors: Cell Biology Research Center, Life Science Research Laboratories, Wako Pure Chemical Industries, Ltd. 6-1 Takada-cho, Amagasaki, Hyogo 661-0963, Japan.
authors: Susumu
authors: S
authors: Honda
authors: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan.
authors: Makoto
authors: M
authors: Asashima
authors: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 2, 1-1-1 Umezono, Tsukuba, Ibaraki 305-8568, Japan.
authors: Jun
authors: J
authors: Hirabayashi
mesh_terms: D001710:Biotin / Q000737:chemistry; D019921:Biotinylation; D057508:Burkholderia cenocepacia / Q000378:metabolism; D002454:Cell Differentiation; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D018920:Coculture Techniques; D005434:Flow Cytometry; D006031:Glycosylation; D057809:HEK293 Cells; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008024:Ligands; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D040081:Protein Array Analysis / Q000379:methods; D012795:Sialoglycoproteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: C041433:Agaricus lectins; D037102:Lectins; D008024:Ligands; D012795:Sialoglycoproteins; C001643:podocalyxin; D001710:Biotin