title: Optical methods for the measurement and manipulation of cytosolic calcium signals in neutrophils.
abstract: The measurement and manipulation of cytosolic free Ca(2+) of neutrophils is crucial for investigating the mechanisms within living neutrophils which generate Ca(2+) signals and the cellular responses triggered by them. Optical methods for this are the most applicable for neutrophils and are discussed here, especially the use of fluorescent indicators of Ca(2+) and photoactivation of reagents involved in Ca(2+) signaling. Both of these synthetic agents can be loaded into neutrophils as lipid-soluble esters or can be microinjected into the cell. In this chapter, we will outline some of the techniques that have been used to monitor, visualize, and manipulate Ca(2+) in neutrophils.
authors: Neutrophil Signalling Group, School of Medicine, Cardiff University, Cardiff, UK.
authors: Maurice B
authors: MB
authors: Hallett
authors: Maha
authors: M
authors: Al-Jumaa
authors: Sharon
authors: S
authors: Dewitt
mesh_terms: D020013:Calcium Signaling / Q000502:physiology*; D003600:Cytosol / Q000378:metabolism; D005434:Flow Cytometry; D005470:Fluorometry / Q000379:methods; D006801:Humans; D009504:Neutrophils / Q000378:metabolism*