title: Cytotoxicity and mutagenicity studies on migration extracts from nanocomposites with potential use in food packaging.
abstract: Clays are used in the food packaging industry to obtain nanocomposites. The use of these new materials is a concern, because they could reach consumers by oral exposure through possible migration, and potential toxic effects could be derived. In the present study, several in vitro basal cytotoxicity and mutagenicity tests on migration extracts obtained from a nanocomposite material with poly (lactic) acid (PLA) and two modified clays, Clay1 and Clay2, are shown. Migration extracts in distilled water showed values of 0.1 ± 0.2mg/dm(2) in all samples. Also, the content of characteristic metals of the clays structure (Al, Ca, Mg, Fe, Si) was studied and no statistical differences were observed. For the cytotoxicity assays, the human intestinal Caco-2 and human liver HepG2 cells were selected. Cells were exposed to concentrations between 2.5% and 100% extracts determining three different biomarkers of cellular viability. No significant differences were observed in the cytotoxicity assays. Finally, mutagenicity was evaluated by the Ames test and resulted in the absence of mutagenic response at all the concentrations assayed. Taking in account all above mentioned, these new materials show a good profile for their use in food packaging although further research is still needed.
authors: Area of Toxicology, Faculty of Pharmacy, Universidad de Sevilla, Professor García González n° 2, 41012 Seville, Spain.
authors: Sara
authors: S
authors: Maisanaba
authors: Area of Toxicology, Faculty of Pharmacy, Universidad de Sevilla, Professor García González n° 2, 41012 Seville, Spain.
authors: Silvia
authors: S
authors: Pichardo
authors: Packaging, Transport, & Logistics Research Institute (ITENE), C/Albert Einstein, 1, Parque Tecnológico, Paterna, Spain.
authors: María
authors: M
authors: Jordá-Beneyto
authors: Packaging, Transport, & Logistics Research Institute (ITENE), C/Albert Einstein, 1, Parque Tecnológico, Paterna, Spain.
authors: Susana
authors: S
authors: Aucejo
authors: Area of Toxicology, Faculty of Pharmacy, Universidad de Sevilla, Professor García González n° 2, 41012 Seville, Spain.
authors: Ana M
authors: AM
authors: Cameán
authors: Area of Toxicology, Faculty of Pharmacy, Universidad de Sevilla, Professor García González n° 2, 41012 Seville, Spain. Electronic address: angelesjos@us.es.
authors: Ángeles
authors: Á
authors: Jos
mesh_terms: D015197:Carcinogenicity Tests; D045744:Cell Line, Tumor; D018857:Food Packaging; D006801:Humans; D009152:Mutagenicity Tests; D053761:Nanocomposites
keywords: Clay; Cytotoxicity; Migration; Mutagenicity; Nanocomposites