title: FtsZDr, a tubulin homologue in radioresistant bacterium Deinococcus radiodurans is characterized as a GTPase exhibiting polymerization/depolymerization dynamics in vitro and FtsZ ring formation in vivo.
abstract: The GTPase-dependent polymerization/depolymerization dynamics of FtsZ regulate bacterial cell division in vivo. Deinococcus radiodurans is better known for its extraordinary radioresistance and therefore, the characterization of FtsZ of this bacterium (FtsZDr) would be required to understand the mechanisms underlying regulation of cell division in response to DNA damage. Recombinant FtsZDr bound to GTP and showed GTPase activity. It produced bundles of protofilaments in the presence of either GTP or Mg2+ ions. But the formation of the higher size ordered structures required both GTP and Mg2+ in vitro. It showed polymerization/depolymerization dynamics as a function of GTP and Mg2+. Interestingly, ATP interacted with FtsZDr and stimulated its GTPase activity by ∼2-fold possibly by increasing both substrate affinity and rate of reaction. FtsZDr-GFP expressing in D. radiodurans produced typical Z ring perpendicular to the plane of first cell division. These results suggested that FtsZDr is a GTPase in vitro and produces typical Z ring at the mid cell position in D. radiodurans.
authors: Molecular Biology Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
authors: Kruti Mehta
authors: KM
authors: Modi
authors: Material Science Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
authors: Raghvendra
authors: R
authors: Tewari
authors: Molecular Biology Division, Bhabha Atomic Research Centre, Mumbai 400085, India. Electronic address: hsmisra@barc.gov.in.
authors: Hari Sharan
authors: HS
authors: Misra
mesh_terms: D058491:Bacterial Load; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D034301:Deinococcus / Q000201:enzymology* / Q000528:radiation effects*; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D058105:Polymerization; D011836:Radiation Tolerance; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; C071525:FtsZ protein, Bacteria; D011994:Recombinant Proteins; D020558:GTP Phosphohydrolases
keywords: Bacterial cell division; Deinococcus; FtsZ-GTPase; Protein polymerization; Radioresistance