title: Three-dimensional structure of bovine heart NADH: ubiquinone oxidoreductase (complex I) by electron microscopy of a single negatively stained two-dimensional crystal.
abstract: Bovine heart NADH:ubiquinone oxidoreductase (complex I), which is the largest (about 1 MDa) membrane protein complex in the mitochondrial respiratory chain, catalyzes the electron transfer from NADH to ubiquinone, coupled with proton pumping. We have crystallized bovine complex I in reconstituted lipid bilayers and obtained a three-dimensional density map by the electron crystallographic analysis of a single negatively stained two-dimensional crystal. The asymmetric unit with dimensions of a = 388 Å, b = 129 Å and γ = 90° contains two molecules and is of P1 symmetry. Structural differences between the two molecules indicate flexibility of the hydrophilic domain relative to the membrane-embedded domain.
authors: Picobiology Institute.
authors: Satoru
authors: S
authors: Shimada
authors: Kyoko
authors: K
authors: Shinzawa-Itoh
authors: Satoko
authors: S
authors: Amano
authors: Yui
authors: Y
authors: Akira
authors: Atsuo
authors: A
authors: Miyazawa
authors: Tomitake
authors: T
authors: Tsukihara
authors: Kazutoshi
authors: K
authors: Tani
authors: Christoph
authors: C
authors: Gerle
authors: Shinya
authors: S
authors: Yoshikawa
mesh_terms: D000818:Animals; D002417:Cattle; D003460:Crystallization; D003461:Crystallography; D004579:Electron Transport; D042967:Electron Transport Complex I / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008928:Mitochondria / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology*; D009243:NAD / Q000737:chemistry; D011487:Protein Conformation; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D009243:NAD; D014451:Ubiquinone; D042967:Electron Transport Complex I
keywords: electron crystallography; electron transfer; membrane protein; mitochondrial respiration; proton pump; tilt series