title: Dynamic monitoring of optical clearing of skin using photoacoustic microscopy and ultrasonography.
abstract: Tissue optical clearing technique has shown great potential for enhancing the imaging depth and contrast of optical imaging modalities. However, the mechanism of optical clearing is still in controversy. In this manuscript, we combined photoacoustic microscopy with ultrasonography to monitor the dermic changes induced by optical clearing agents at different immersion time points. The measured parameters were correlated with the optical clearing process, and could be used to assess the optical clearing effect. Both in vitro and in vivo results demonstrated that photoacoustic microscopy and ultrasonography can potentially be used as a powerful tool in screening optical clearing agents and exploring the mechanism of optical clearing.
authors: Xiaoquan
authors: X
authors: Yang
authors: Yanyan
authors: Y
authors: Liu
authors: Dan
authors: D
authors: Zhu
authors: Rui
authors: R
authors: Shi
authors: Qingming
authors: Q
authors: Luo
mesh_terms: D000287:Administration, Topical; D000818:Animals; D003879:Dermatologic Agents / Q000008:administration & dosage; D046169:Dermoscopy / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D054459:Elasticity Imaging Techniques / Q000295:instrumentation*; D005990:Glycerol / Q000008:administration & dosage*; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation*; D033401:Microscopy, Acoustic / Q000295:instrumentation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000000981:diagnostic imaging* / Q000187:drug effects; D012880:Skin Pigmentation / Q000187:drug effects / Q000502:physiology*
chemical_list: D003879:Dermatologic Agents; D005990:Glycerol