title: In vitro antioxidant activity and potential inhibitory action against α-glucosidase of polysaccharides from fruit peel of tea (Camellia sinensis L.).
abstract: The conditions for extracting polysaccharides from tea (Camellia sinensis L.) fruit peel (TFPPs) were studied. Three parameters (temperature, time, and liquid/solid ratio) affecting the extraction of TFPP were optimized using response surface methodology (RSM). Under the optimized conditions, the yield of TFPP was predicted to be 4.98%. The physicochemical properties, in vitro antioxidant activities, and inhibitory effects on α-glucosidase of fractionated TFPPs (TFPP-0, TFPP-20, TFPP-40, and TFPP-60) were investigated. We found that the TFPPs were all acid protein-bound heteropolysaccharides, although with different chemical compositions. They had not only remarkable scavenging activity on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing activity, but also excellent inhibitory potential against α-glucosidase in vitro. Our results suggest that tea fruit peel could be treated as a potential bioresource for the development of polysaccharide antioxidants.
authors: Department of Tea Science, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture, Hangzhou 310058, China; College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors: Yue-fei
authors: YF
authors: Wang
authors: Jie
authors: J
authors: Wang
authors: Jing
authors: J
authors: Wu
authors: Ping
authors: P
authors: Xu
authors: Yi-qi
authors: YQ
authors: Wang
authors: Jun-jie
authors: JJ
authors: Gao
authors: Danielle
authors: D
authors: Hochstetter
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D028241:Camellia sinensis / Q000737:chemistry*; D002241:Carbohydrates / Q000737:chemistry; D002614:Chelating Agents / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004791:Enzyme Inhibitors / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry; D065089:Glycoside Hydrolase Inhibitors; D011134:Polysaccharides / Q000737:chemistry*; D013662:Tea / Q000737:chemistry*; D000520:alpha-Glucosidases / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D002241:Carbohydrates; D002614:Chelating Agents; D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D011134:Polysaccharides; D013662:Tea; D000431:Ethanol; D000520:alpha-Glucosidases