title: A sensitive LC-MS/MS method for simultaneous determination of amygdalin and paeoniflorin in human plasma and its application.
abstract: A simple and sensitive HPLC-MS/MS method was developed and fully validated for the simultaneous determination of amygdalin (AD) and paeoniflorin (PF) in human plasma. For both analytes, the method exhibited high sensitivity (LLOQs of 0.6ng/mL) by selecting the ammonium adduct ions ([M+NH4](+)) as the precursor ions and good linearity over the concentration range of 0.6-2000ng/mL with the correlation coefficients>0.9972. The intra- and inter-day precision was lower than 10% in relation to relative standard deviation, while accuracy was within ±2.3% in terms of relative error for both analytes. The developed method was successfully applied to a pilot pharmacokinetic study of AD and PF in healthy volunteers after intravenous infusion administration of Huoxue-Tongluo lyophilized powder for injection.
authors: Department of Pharmaceutical Analysis, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China; Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China; Department of Analytical Chemistry, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China.
authors: Xiaobing
authors: X
authors: Li
authors: Department of Pharmaceutical Analysis, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China; Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China.
authors: Fuguo
authors: F
authors: Shi
authors: Department of Pharmaceutical Analysis, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China; Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China.
authors: Pan
authors: P
authors: Gu
authors: Department of Pharmaceutical Analysis, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China; Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China.
authors: Lingye
authors: L
authors: Liu
authors: Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China; Department of Analytical Chemistry, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China. Electronic address: dochehua@163.com.
authors: Hua
authors: H
authors: He
authors: Department of Pharmaceutical Analysis, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China; Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China. Electronic address: dinglidl@hotmail.com.
authors: Li
authors: L
authors: Ding
mesh_terms: D000678:Amygdalin / Q000097:blood* / Q000737:chemistry* / Q000493:pharmacokinetics; D002853:Chromatography, Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005960:Glucosides / Q000097:blood* / Q000737:chemistry* / Q000493:pharmacokinetics; D006801:Humans; D039821:Monoterpenes / Q000097:blood* / Q000737:chemistry* / Q000493:pharmacokinetics; D010865:Pilot Projects; D053719:Tandem Mass Spectrometry / Q000379:methods*; D006027:Glycosides
chemical_list: D000678:Amygdalin; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D039821:Monoterpenes; C527174:tongluo; C015423:peoniflorin; D006027:Glycosides
keywords: Amygdalin; Huoxue-Tongluo lyophilized powder for injection; LC–MS/MS; Paeoniflorin; Pharmacokinetics