title: Micrometastasis expressing insulin arise in lung and spleen at advanced stage of rip1-tag2 transgenic mice.
abstract: Rip1-Tag2 mice is one overt pancreatic β-cell tumor model, which is widely used for studying pancreas tumor angiogenesis and tumor development. However, tumor metastasis in Rip1-Tag2 mice had rarely been reported, in this present study, we find some micrometastasis in lung and spleen of the Rip1-Tag2 mice at advanced stage, which is important for uncovering metastasis cell characteristics and exploring how to survive in cancer microenvironment. To study the micrometastasis of Rip1-Tag2 mice in advanced pancreatic cancer, we first observed the pathology process of β cell tumor in Rip1-Tag2 mice through HE staining, then we performed immunohistochemistry with insulin antibody, T-antigen antibodies and C-petide antibody on lung and spleen tissues sections from advanced stage, comparing with background wild-type C57BL/6 mice sections. The results indicated that micrometastasis expressing insulin was found in the Rip1-Tag2 mice lung, and spleen. Further evidences demonstrate pathology structure of lung and spleen are damaged. Interestingly and importantly, the expression of T antigen and insulin antibodies are all decreased in advanced stage of primary β cell tumor, which suggest that the at least partly micrometastasis is derived from the early stage or from advanced stage of β cell tumor then return to undifferentiated state like cancer stem cell. The findings contributed to the study of cancer metastasis and cancer stem cell.
authors: 1. Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou 510006, China;
authors: Jiang-chao
authors: JC
authors: Li
authors: 2. School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou, 510006, Guangdong, China;
authors: Yongxia
authors: Y
authors: Yang
authors: 2. School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou, 510006, Guangdong, China;
authors: Xiao-ming
authors: XM
authors: Zhou
authors: 1. Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou 510006, China;
authors: Si-mei
authors: SM
authors: Guo
authors: 1. Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou 510006, China;
authors: Ying
authors: Y
authors: Liu
authors: 1. Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou 510006, China;
authors: Cui-ling
authors: CL
authors: Qi
authors: 1. Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou 510006, China;
authors: Tian
authors: T
authors: Lan
authors: 1. Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou 510006, China;
authors: Qian-qian
authors: QQ
authors: Zhang
authors: 1. Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou 510006, China;
authors: Li-jing
authors: LJ
authors: Wang
mesh_terms: D000818:Animals; D000957:Antigens, Viral, Tumor / Q000378:metabolism; D015536:Down-Regulation; D020690:GTPase-Activating Proteins / Q000235:genetics*; D007328:Insulin / Q000378:metabolism*; D007340:Insulinoma / Q000235:genetics* / Q000378:metabolism; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D061206:Neoplasm Micrometastasis; D013154:Spleen / Q000378:metabolism* / Q000473:pathology; D059016:Tumor Microenvironment
chemical_list: D000957:Antigens, Viral, Tumor; D020690:GTPase-Activating Proteins; D007328:Insulin; C490620:Ralbp1 protein, mouse
keywords: Rip1-Tag2 transgenic mice; insulin; micrometastasis