title: Compact portable biosensor for arsenic detection in aqueous samples with Escherichia coli bioreporter cells.
abstract: We present a compact portable biosensor to measure arsenic As(III) concentrations in water using Escherichia coli bioreporter cells. Escherichia coli expresses green fluorescent protein in a linearly dependent manner as a function of the arsenic concentration (between 0 and 100 μg/L). The device accommodates a small polydimethylsiloxane microfluidic chip that holds the agarose-encapsulated bacteria, and a complete optical illumination/collection/detection system for automated quantitative fluorescence measurements. The device is capable of sampling water autonomously, controlling the whole measurement, storing and transmitting data over GSM networks. We demonstrate highly reproducible measurements of arsenic in drinking water at 10 and 50 μg/L within 100 and 80 min, respectively.
authors: Institute of Systems Engineering, University of Applied Sciences and Arts Western Switzerland Valais, Sion, Switzerland.
authors: Frederic
authors: F
authors: Truffer
authors: Laboratory of Microsystems Engineering, Ecole Polytechnique Fédérale Lausanne, EPFL-STI-LMIS, Station 17, CH-1015 Lausanne, Switzerland.
authors: Nina
authors: N
authors: Buffi
authors: Department of Fundamental Microbiology, University of Lausanne, Switzerland.
authors: Davide
authors: D
authors: Merulla
authors: Department of Fundamental Microbiology, University of Lausanne, Switzerland.
authors: Siham
authors: S
authors: Beggah
authors: Laboratory of Microsystems Engineering, Ecole Polytechnique Fédérale Lausanne, EPFL-STI-LMIS, Station 17, CH-1015 Lausanne, Switzerland.
authors: Harald
authors: H
authors: van Lintel
authors: Laboratory of Microsystems Engineering, Ecole Polytechnique Fédérale Lausanne, EPFL-STI-LMIS, Station 17, CH-1015 Lausanne, Switzerland.
authors: Philippe
authors: P
authors: Renaud
authors: Department of Fundamental Microbiology, University of Lausanne, Switzerland.
authors: Jan Roelof
authors: JR
authors: van der Meer
authors: Institute of Systems Engineering, University of Applied Sciences and Arts Western Switzerland Valais, Sion, Switzerland.
authors: Martial
authors: M
authors: Geiser
mesh_terms: D001151:Arsenic / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004926:Escherichia coli / Q000166:cytology*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D014867:Water / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis
chemical_list: D014874:Water Pollutants, Chemical; D014867:Water; D001151:Arsenic