title: Targeted amplification of delivery to cell surface receptors by dendrimer self-assembly.
abstract: Nanometer-scale architectures assembled on cell surface receptors from smaller macromolecular constituents generated a large amplification of fluorescence. A targeted dendrimer was synthesized from a cystamine-core G4 PAMAM dendrimer, and contained an anti-BrE3 monoclonal antibody as the targeting group, several fluorophores and an average of 12 aldehyde moieties as complementary bio-orthogonal reactive sites for the covalent assembly. A cargo dendrimer, derived from a PAMAM G4 dendrimer, contained several fluorophores as the cargo for delivery and five hydrazine moieties as complimentary bio-orthogonal reactive sites. The system is designed to be flexible and allow for facile incorporation of a variety of targeting ligands.
authors: Department of Chemistry, New York University, New York, NY 10003, USA; Nanometics LLC, 111 Great Neck Rd, Suite 212, Great Neck, NY 11021, USA.
authors: Steven
authors: S
authors: Isaacman
authors: Cancer Institute, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA.
authors: Michael
authors: M
authors: Buckley
authors: Department of Chemistry, New York University, New York, NY 10003, USA.
authors: Xiaojian
authors: X
authors: Wang
authors: Department of Radiology, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA.
authors: Edwin Y
authors: EY
authors: Wang
authors: Cancer Institute, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA.
authors: Leonard
authors: L
authors: Liebes
authors: Department of Chemistry, New York University, New York, NY 10003, USA; Cancer Institute, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA. Electronic address: canary@nyu.edu.
authors: James W
authors: JW
authors: Canary
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D045744:Cell Line, Tumor; D050091:Dendrimers / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D009757:Nylons / Q000737:chemistry*; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000378:metabolism*; D012235:Rhodamines / Q000737:chemistry / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D050091:Dendrimers; D004337:Drug Carriers; D005456:Fluorescent Dyes; D009757:Nylons; C520550:PAMAM-G4; D011956:Receptors, Cell Surface; D012235:Rhodamines
keywords: Cell surface self-assembly; Dendrimer; Targeted amplification