title: Bioinspired porous membranes containing polymer nanoparticles for wound healing.
abstract: Skin damages covering a surface larger than 4 cm(2) require a regenerative strategy based on the use of appropriate wound dressing supports to facilitate the rapid tissue replacement and efficient self-healing of the lost or damaged tissue. In the present work, A novel biomimetic approach is proposed for the design of a therapeutic porous construct made of poly(L-lactic acid) (PLLA) fabricated by thermally induced phase separation (TIPS). Biomimicry of ECM was achieved by immobilization of type I collagen through a two-step plasma treatment for wound healing. Anti-inflammatory (indomethacin)-containing polymeric nanoparticles (nps) were loaded within the porous membranes in order to minimize undesired cell response caused by post-operative inflammation. The biological response to the scaffold was analyzed by using human keratinocytes cell cultures. In this work, a promising biomimetic construct for wound healing and soft tissue regeneration with drug-release properties was fabricated since it shows (i) proper porosity, pore size, and mechanical properties, (ii) biomimicry of ECM, and (iii) therapeutic potential.
authors: Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129, Turin, Italy.
authors: Ana M
authors: AM
authors: Ferreira
authors: Clara
authors: C
authors: Mattu
authors: Elia
authors: E
authors: Ranzato
authors: Gianluca
authors: G
authors: Ciardelli
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry / Q000494:pharmacology; D002461:Cell Line, Transformed; D024042:Collagen Type I; D005109:Extracellular Matrix / Q000737:chemistry; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism* / Q000473:pathology; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology; D008422:Materials Testing; D008567:Membranes, Artificial; D053758:Nanoparticles / Q000737:chemistry*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology; D016062:Porosity; D012867:Skin / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D024042:Collagen Type I; D008567:Membranes, Artificial; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)
keywords: collagen; nanoparticles; plasma; porous membrane; wound healing