title: [Study of resveratrol suppressing TGF-beta1 induced transdifferentiation of podocytes].
abstract: OBJECTIVE
  To explore the effect of resveratrol on transforming growth factor-beta1 (TGF-beta1) induced transdifferentiation of podocytes.
  
  
  METHODS
  Mouse podocytes in vitro cultured under differentiating conditions for 10 days were divided into the normal group, the model group, the high dose resveratrol group, and the low dose resveratrol group. The podocytes in the high and low dose resveratrol groups were intervened with 5 micromol/L and 2 micromol/L resveratrol respectively for 30 min. Those in the model group and the two resveratrol treated groups were continually incubated with 5 ng/mL TGF-beta1 for 72 h. Those in the normal group were routinely cultured. The protein expression of podocyte phenotypic protein molecules such as E-cadherin, P-cadherin, zonula occludens-1 (ZO-1), NEPH1, and alpha-smooth muscle-actin (alpha-SMA) were detected by immunocytochemistry, flow cytometry (FCM), and Western blot. A simple albumin influx assay was used to evaluate the filtration barrier function of podocyte monolayer.
  
  
  RESULTS
  Compared with the normal control group, E-cadherin (+) percentage rate, the protein expression of P-cadherin, ZO-1, and NEPH1 significantly decreased in the model group (P < 0.05), but the expression of alpha-SMA and albumin permeability across podocyte monolayers increased significantly (P < 0.05). Compared with the model group, E-cadherin (+) percentage rate significantly increased (P < 0.05) and albumin permeability across podocyte monolayers decreased significantly (P < 0.05) in the high and low dose resveratrol groups. In the low dose resveratrol group, the expression of P-cadherin and NEPH1 significantly increased (P < 0.05). In the high dose resveratrol group, the expression of P-cadherin, ZO-1, and NEPH1 increased significantly, and the expression of alpha-SMA decreased significantly (P < 0.05). The correlations between resveratrol concentrations and the expression of E-cadherin (+), P-cadherin, and NEPH1 were significantly positive (r(E-cadherin (+)) = 0.772, r(P-cadherin) = 0.756, r(NEPH1) = 0.809, P < 0.05).
  
  
  CONCLUSIONS
  The role of resveratrol in inhibiting TGF-beta1 induced phenotype abnormality might be an important mechanism for preserving the integrality of glomerular filtration barrier and decreasing proteinuria.
authors: Laboratory of Nephropathy, Hangzhou Municipal Hospital of Traditional Chinese Medicine, Hangzhou 310007, China. yangruchunhz@163.com
authors: Ru-Chun
authors: RC
authors: Yang
authors: Laboratory of Nephropathy, Hangzhou Municipal Hospital of Traditional Chinese Medicine, Hangzhou 310007, China.
authors: Xiao-Ling
authors: XL
authors: Zhu
authors: Laboratory of Nephropathy, Hangzhou Municipal Hospital of Traditional Chinese Medicine, Hangzhou 310007, China.
authors: Hua-Qin
authors: HQ
authors: Zhang
authors: Laboratory of Nephropathy, Hangzhou Municipal Hospital of Traditional Chinese Medicine, Hangzhou 310007, China.
authors: Wei-Dong
authors: WD
authors: Li
mesh_terms: D000818:Animals; D054338:Cell Transdifferentiation / Q000187:drug effects*; D002478:Cells, Cultured; D007684:Kidney Tubules / Q000166:cytology / Q000187:drug effects; D051379:Mice; D050199:Podocytes / Q000166:cytology* / Q000187:drug effects*; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D013267:Stilbenes; D053773:Transforming Growth Factor beta1; D000077185:Resveratrol