title: The PGRS domain is responsible for translocation of PE_PGRS30 to cell poles while the PE and the C-terminal domains localize it to the cell wall.
abstract: PE_PGRS proteins localize in the mycobacterial cell wall and the cell wall localization of PE_PGRS33 has been shown to be attributed to its PE domain. In this study, we expressed deletion mutants of PE_PGRS30 in Mycobacterium smegmatis to characterize the role of its domains in protein localization. It was revealed that, apart from the PE domain, the C-terminal domain present in few PE_PGRS proteins carries individual cell wall localization signals. Proteinase K sensitivity assay showed that PE_PGRS30 is exposed on the mycobacterial surface through its PGRS domain. PGRS domain was also shown to be responsible for polar localization of PE_PGRS30.
authors: Gene Regulation Laboratory, National Institute of Immunology, New Delhi 110067, India.
authors: Shweta
authors: S
authors: Chatrath
authors: Gene Regulation Laboratory, National Institute of Immunology, New Delhi 110067, India.
authors: Vineet Kumar
authors: VK
authors: Gupta
authors: Gene Regulation Laboratory, National Institute of Immunology, New Delhi 110067, India. Electronic address: lalit@nii.ac.in.
authors: Lalit C
authors: LC
authors: Garg
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D002458:Cell Fractionation; D002473:Cell Wall / Q000378:metabolism*; D008969:Molecular Sequence Data; D020102:Mycobacterium smegmatis / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D021381:Protein Transport
chemical_list: D001426:Bacterial Proteins
keywords: C-terminal domain; Localization; Mycobacterium tuberculosis; PE domain; PE_PGRS30; PGRS domain