title: Prevalence of metilentetrahidrofolate reductase C677T polymorphism, consumption of vitamins B6, B9, B12 and determination of lipidic hydroperoxides in obese and normal weight Mexican population.
abstract: BACKGROUND
  Oxidative stress is a key factor in the development of the principal comorbidities of obesity. Methylenetetrahydrofolate reductase enzyme (MTHFR) participates in the metabolism of folate with the action of vitamins B6 and B12. The gene of MTHFR may present a single nucleotide polymorphism (SNP) at position 677 (C677T), which can promote homocysteinemia associated to the production of free radicals.
  
  
  OBJECTIVE
  To determine the frequency of SNP C677T of the MTHFR, evaluate the consumption of vitamins B6, B9, B12 and determine the concentration of plasma lipid hydroperoxides (LOOH) in obese and control groups.
  
  
  METHODS
  128 Mexican mestizo according to their body mass index were classified as normal weight (Nw; n=75) and obesity (ObeI-III; n=53). Identification of SNP C677T of MTHFR was performed by PCR-RFLP technic. The consumption of vitamins B6, B9 and B12 was assessed by a validate survey. LOOH was determined as an indicator of peripheral oxidative stress.
  
  
  RESULTS
  There was no statistical difference in the frequency of the C677T polymorphism between the TT homozygous genotype in Nw (0.19) and ObeI-III (0.25). The frequency of T allele in Nw was 0.45 and 0.51 in ObI-III group. There were no statistical differences in the consumption of vitamins B6, B9 and B12 between Nw and ObI-III groups. The LOOH showed statistical difference (p < 0.05) between Nw and ObI–III group.
  
  
  CONCLUSIONS
  Oxidative stress is present in all grades of obesity although there were no differences in the vitamin consumption and the SNP C677T between Nw and ObeI–III groups.
authors: Departamento de Salud, Universidad Iberoamericana Ciudad de México. México.. cesar.hernandez@uia.mx.
authors: César
authors: C
authors: Hernández-Guerrero
authors: Estudiante de la Maestría en Nutriología Aplicada de la Universidad Iberoamericana Ciudad de México. México..
authors: Inés
authors: I
authors: Romo-Palafox
authors: Departamento de Salud, Universidad Iberoamericana Ciudad de México. México..
authors: Mary Carmen
authors: MC
authors: Díaz-Gutiérrez
authors: Estudiante de la Licenciatura en Nutrición y Ciencia de los Alimentos de la Universidad Iberoamericana Ciudad de México. México..
authors: Mariana
authors: M
authors: Iturbe-García
authors: Estudiante de la Licenciatura en Nutrición y Ciencia de los Alimentos de la Universidad Iberoamericana Ciudad de México. México..
authors: Alejandra
authors: A
authors: Texcahua-Salazar
authors: Departamento de Salud, Universidad Iberoamericana Ciudad de México. México..
authors: Ana Bertha
authors: AB
authors: Pérez-Lizaur
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D005492:Folic Acid / Q000008:administration & dosage*; D005787:Gene Frequency; D006801:Humans; D008054:Lipid Peroxides / Q000097:blood*; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008800:Mexico / Q000453:epidemiology; D008875:Middle Aged; D009765:Obesity / Q000097:blood* / Q000453:epidemiology / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics; D015995:Prevalence; D014805:Vitamin B 12 / Q000008:administration & dosage*; D025101:Vitamin B 6 / Q000008:administration & dosage*; D055815:Young Adult
chemical_list: D008054:Lipid Peroxides; D025101:Vitamin B 6; D005492:Folic Acid; D042965:Methylenetetrahydrofolate Reductase (NADPH2); D014805:Vitamin B 12