title: Optimization of polysaccharides extraction from seeds of Pharbitis nil and its anti-oxidant activity.
abstract: In the present study, polysaccharides were extracted from Pharbitis nil seeds (PNSs) by ultrasonic extraction method for the first time. Response surface methodology with Box-Behnken design was used to optimize the extraction process of PNS polysaccharides (PNSPs). Highest PNSPs yield 6.01 ± 0.15% that agreed closely with the predicted yield 5.99% were obtained under the optimal conditions as follows: ratio of water to raw material 6.5 mL/g, extraction temperature 49.0 °C, ultrasonic power 61.6 W, and extraction time 32.6 min. Preliminary characterization indicated that the sugar, uronic acid and protein contents of the product were 83.6 ± 1.61, 21.8 ± 1.25 and 16.4 ± 0.88% (w/w), respectively. FT-IR analysis revealed the general characteristic absorption peaks of polysaccharides. Besides, PNSPs showed a remarkable antioxidant activity in vitro in a dose-dependent manner. These may provide theoretical basis for further system research and rational development and utilization of PNS resources.
authors: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150040, China; Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
authors: Qiuhong
authors: Q
authors: Wang
authors: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150040, China.
authors: Yanping
authors: Y
authors: Sun
authors: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150040, China.
authors: Bingyou
authors: B
authors: Yang
authors: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150040, China.
authors: Zhibin
authors: Z
authors: Wang
authors: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150040, China.
authors: Yanxin
authors: Y
authors: Liu
authors: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150040, China.
authors: Qi
authors: Q
authors: Cao
authors: Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China. Electronic address: sun_xiaobo163@163.com.
authors: Xiaobo
authors: X
authors: Sun
authors: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150040, China. Electronic address: hxkuang@hotmail.com.
authors: Haixue
authors: H
authors: Kuang
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D049915:Ipomoea nil / Q000196:embryology*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000975:Antioxidants; D011134:Polysaccharides
keywords: Anti-oxidant activity; Polysaccharides; Preliminary characterization; Seeds of Pharbitis nil; Ultrasonic extraction