title: Synthesis, and In-vitro Cytotoxicity Studies of a Series of Triazene Derivatives on Human Cancer Cell Lines.
abstract: Compounds containing triazene ring structure are cytotoxic agents and clinically used as antitumor alkylating agents. In this study, a series of triazene derivatives holding alkyl and aryl moieties were synthesized and proved to be potent cytotoxic agents in-vitro particularly against eight cancer cell lines (PC3, HT29, Hela, HL60, Jurkat, K562, MCF7, HepG2) and a non-cancerous cell line (HUVEC). The cytotoxic activity was assessed using two methods, LDH assay, and trypan blue exclusion. Some of the triazene derivatives showed cytotoxic activity more than temozolomide (TMZ) as the reference drug. The synthesized triazenes showed marked cytotoxicity effects on all eight cancer cell lines. Among the compounds synthesized, 1,3-bis(2-ethoxyphenyl)triazene C had unique efficacy and selectivity so that it had IC50 between 0.560-3.33 μM on cancer cell lines and 12.61 μM on normal cell line (HUVEC). 1-(4-nitrophenyl)-3-(2-hydroxyethyl)triazene E shows weaker effect on cancer cell lines than the other compounds having IC50 between 3-15.54 μM.
authors: Novel Drug Delivery Research Center, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors: Hadi
authors: H
authors: Adibi
authors: Student's Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran. ; Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors: Mohammad Bagher
authors: MB
authors: Majnooni
authors: Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors: Ali
authors: A
authors: Mostafaie
authors: Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors: Kamran
authors: K
authors: Mansouri
authors: Analytical Chemistry Department, Faculty of Chemistry, Razi University, Kermanshah, Iran.
authors: Moslem
authors: M
authors: Mohammadi
keywords: Cancer cell lines; Cytotoxic activity; LDH assay; Triazene derivatives; Trypan blue exclusion