title: Study on antimicrobial potential of neem oil nanoemulsion against Pseudomonas aeruginosa infection in Labeo rohita.
abstract: Presence of several biochemical constituents in neem makes it an efficient antimicrobial agent for pathogenic diseases. The current investigation was aimed to assess the therapeutic potential of neem nanoemulsion as a control measure for Pseudomonas aeruginosa infection in freshwater fish Labeo rohita. The median lethal concentration (LC50) for the neem oil and neem nanoemulsion was 73.9 and 160.3 mg/L, respectively. The biomarker enzymes of treated fish tissues showed a significant difference in the level of glutathione reductase, catalase, and lipid peroxidation in neem oil-treated samples than in neem nanoemulsion-treated samples at P<0.05. The results were corroborative with histopathology and ultrastructural analysis. The bacterial infection of P. aeruginosa treated using neem nanoemulsion was more effective in both in vitro and in vivo methods. Present findings suggest that neem-based nanoemulsion has negligible toxicity to Rohu fishes. This makes neem-based nanoemulsion as an efficient therapeutic agent against P. aeruginosa infection, leading to its possible usage in the aquaculture industry.
authors: Centre for Nanobiotechnology, VIT University, Vellore, Tamil Nadu, India.
authors: Prabhakar
authors: P
authors: Mishra
authors: Suresh Kumar
authors: SK
authors: R S
authors: Jayakumar
authors: J
authors: Jerobin
authors: John
authors: J
authors: Thomas
authors: Amitava
authors: A
authors: Mukherjee
authors: Natarajan
authors: N
authors: Chandrasekaran
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D003530:Cyprinidae / Q000382:microbiology*; D004655:Emulsions / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D005393:Fish Diseases / Q000382:microbiology; D005880:Gills / Q000382:microbiology / Q000473:pathology; D005989:Glycerides / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D049329:Nanostructures / Q000737:chemistry / Q000633:toxicity; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D013729:Terpenes / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D000900:Anti-Bacterial Agents; D004655:Emulsions; D005989:Glycerides; D013729:Terpenes; C002443:neem oil
keywords: Labeo rohita; Pseudomonas aeruginosa; biosafety; histopathology; neem nanoemulsion