title: Cytotoxic activity of methanolic extract of Mentha longifolia and Ocimum basilicum against human breast cancer.
abstract: Labiatae family is represented in Saudi Arabia. The aim of the present study was to go insight to investigate the anticancer activity and antioxidative potentials of methanolic extracts of Mentha longifolia L. (ML) and Ocimum basilicum L. (OB) that grown in Madina province, western region, Saudi Arabia. OB exhibited the greater phenolic contents as mg gallic acid equivalent/g weight (mg GAE/g) for a value of 105 +/- 5.5 mg GAE/g. On the other hand, ML produced 29 +/- 3.12 mg GAE/g. The standard antioxidant vitamin E used in this experiment elicited a value of total phenolic contents equal 22 +/- 2.2 mg GAE/g. The percentage scavenging activity of against diphenylpicrylhydrazyl (DPPH) was 850 and 160% for OB and ML extracts, respectively. Vitamin E elicited% scavenging activity of against DPPH equal to 198%. Brine shrimp cytotoxic assay clearly indicated the cytotoxic effects of either ML or OB extract. The brine shrimp survival is inversely proportional to the concentration of either ML or OB extract used with LD50 191.23 and 235.50 ppm, respectively. Toxic effects on brine shrimps indicated the anticancer potential of ML or OB extract. The ML or OB extract was unable to produce pbluescript (pBS) plasmid DNA damage, while the plasmid DNA treated with EcoRI produced a single band as a result of DNA damage. Also, both ML and OB extract exhibited marked cytotoxic activity against MCF-7 cells at various concentrations (20, 40, 80, 160 and 320 microg mL(-1)). The 160 and 320 microg mL(-1) showed more cytotoxic effect against MCF-7 cells. Based on results achieved, we can concluded that, OB and ML extracts have the potency to act as powerful antioxidants and protect against DNA damage and have cytotoxic activity against MCF-7 cell line.
authors: Department of Medical Laboratories Technology, Faculty of Applied Medical Sciences, Taibah University, Madinah, P.O. Box 30001, Saudi Arabia.
authors: Khalil H
authors: KH
authors: Al-Ali
authors: Department of Medical Laboratories Technology, Faculty of Applied Medical Sciences, Taibah University, Madinah, P.O. Box 30001, Saudi Arabia.
authors: Hesham A
authors: HA
authors: El-Beshbishy
authors: Department of Medical Parasitology, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors: Ayman A
authors: AA
authors: El-Badry
authors: Department of Biochemistry, Faculty of Medicine, Kuwait University, Safat 13110, P.O. Box 24923, Kuwait.
authors: Moussa
authors: M
authors: Alkhalaf
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D000975:Antioxidants / Q000494:pharmacology; D001156:Artemia / Q000187:drug effects; D001943:Breast Neoplasms / Q000473:pathology*; D049109:Cell Proliferation / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007928:Lethal Dose 50; D061986:MCF-7 Cells; D027541:Mentha / Q000737:chemistry; D000432:Methanol / Q000737:chemistry*; D018649:Ocimum basilicum / Q000737:chemistry; D010636:Phenols / Q000494:pharmacology; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D010946:Plants, Medicinal; D012997:Solvents / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts; D012997:Solvents; D000432:Methanol