title: A mannosylated cell-penetrating peptide-graft-polyethylenimine as a gene delivery vector.
abstract: Polyethylenimine (PEI) is widely applied in non-viral gene delivery vectors. PEI with high molecular weight is highly effective in gene transfection but is high cytotoxic. Conversely, PEI with low molecular weight displays lower cytotoxicity but less delivering efficiency. To overcome this issue, a novel copolymer with mannosylated, a cell-penetrating peptide (CPP), grafting into PEI with molecular weight of 1800 (Man-PEI1800-CPP) were prepared in this study to target antigen-presenting cells (APCs) with mannose receptors and enhance transfection efficiency with grafting CPP. The copolymer was characterized by (1)H NMR and FTIR. Spherical nanoparticles were formed with diameters of about 80-250 nm by mixing the copolymer and DNA at various charge ratios of copolymer/DNA(N/P). Gel retardation assays indicated that Man-PEI1800-CPP polymers efficiently condensed DNA at low N/P ratios. Cytotoxicity studies showed that Man-PEI1800-CPP/DNA complexes maintained in a high percentage of cell viability compared to the PEI with molecular weight of 25 k (PEI25k). Laser scan confocal microscopy and flow cytometry confirmed that Man-PEI1800-CPP/DNA complexes resulted in higher cell uptake efficiency on DC2.4 cells than on Hela cells line. The transfection efficiency of Man-PEI1800-CPP was significantly higher than that of PEI25k on DC2.4 cells. More importantly, the complexes were mainly distributed in the epidermis and dermis of skin and targeted on splenocytes after percutaneous coating based on microneedles in vivo. These results indicated that Man-PEI1800-CPP was a potential APCs targeted of non-virus vector for gene therapy.
authors: College of Pharmaceutical Sciences, Zhejiang University, Yuhangtang Road 388, Hangzhou, Zhejiang Province 310058, China; Zhejiang Pharmaceutical College, Ningbo, Zhejiang, China.
authors: Ying
authors: Y
authors: Hu
authors: Zhejiang Pharmaceutical College, Ningbo, Zhejiang, China.
authors: Beihua
authors: B
authors: Xu
authors: Zhejiang Pharmaceutical College, Ningbo, Zhejiang, China.
authors: Qixiong
authors: Q
authors: Ji
authors: Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou, Zhejiang, China.
authors: Dan
authors: D
authors: Shou
authors: Department of Pharmacy, Zhejiang University City College, Hangzhou, Zhejiang, China.
authors: Xiaoyi
authors: X
authors: Sun
authors: Zhejiang Pharmaceutical College, Ningbo, Zhejiang, China; Department of Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors: Jiaojiao
authors: J
authors: Xu
authors: College of Pharmaceutical Sciences, Zhejiang University, Yuhangtang Road 388, Hangzhou, Zhejiang Province 310058, China.
authors: Jianqing
authors: J
authors: Gao
authors: College of Pharmaceutical Sciences, Zhejiang University, Yuhangtang Road 388, Hangzhou, Zhejiang Province 310058, China. Electronic address: wqliang@zju.edu.cn.
authors: Wenquan
authors: W
authors: Liang
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D057846:Cell-Penetrating Peptides / Q000737:chemistry*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D011094:Polyethyleneimine / Q000737:chemistry*
chemical_list: D057846:Cell-Penetrating Peptides; D011094:Polyethyleneimine
keywords: APCs-targeted gene delivery vector; Cell-penetrating peptides (CPPs); Mannose; Polyethylenimine (PEI)