title: Contribution of cerebrospinal fluid sCD19 levels to the detection of CNS lymphoma and its impact on disease outcome.
abstract: Flow cytometry (FCM) is more sensitive than conventional cytology for detection of occult leptomeningeal lymphoma; however, some FCM-negative patients show central nervous system (CNS) recurrence. Here, we evaluated the cerebrospinal fluid (CSF) levels of 13 B-cell-associated markers and their contribution to the diagnosis of CNS lymphoma in 91 diffuse large B-cell lymphomas (DLBCL) and 22 Burkitt lymphomas (BLs). From all markers tested, CD19 was the most informative. Thus, higher soluble CD19 (sCD19) levels were associated with a greater frequency of neurological symptoms in DLBCL and BL and with parenchymal CNS lymphoma in DLBCL; sCD19 emerged as a powerful predictor of event-free and overall survival in DLBCL and BL, particularly when combined with FCM detection of CNS disease. These results support the utility of combined FCM detection of lymphoma cells and assessment of sCD19 levels in CSF, for more accurate identification of CNS disease in DLBCL and BL patients.
authors: Department of Medicine and Centro de Investigación del Cáncer (Instituto de Biología Molecular y Celular del Cáncer, Consejo Superior de Investigaciones Científicas/Universidad de Salamanca) and Department of Hematology, University Hospital, Instituto de Investigación Biomédica de Salamanca and University of Salamanca, Salamanca, Spain;
authors: Carmen
authors: C
authors: Muñiz
authors: Lourdes
authors: L
authors: Martín-Martín
authors: Antonio
authors: A
authors: López
authors: Blanca
authors: B
authors: Sánchez-González
authors: Antonio
authors: A
authors: Salar
authors: Julia
authors: J
authors: Almeida
authors: Juan-Manuel
authors: JM
authors: Sancho
authors: José María
authors: JM
authors: Ribera
authors: Cecilia
authors: C
authors: Heras
authors: Francisco Javier
authors: FJ
authors: Peñalver
authors: Marta
authors: M
authors: Gómez
authors: Eva
authors: E
authors: González-Barca
authors: Natalia
authors: N
authors: Alonso
authors: Belén
authors: B
authors: Navarro
authors: Teresa
authors: T
authors: Olave
authors: Francisco
authors: F
authors: Sala
authors: Eulogio
authors: E
authors: Conde
authors: José Antonio
authors: JA
authors: Márquez
authors: Elena
authors: E
authors: Cabezudo
authors: Antonia
authors: A
authors: Cladera
authors: María
authors: M
authors: García-Malo
authors: María Dolores
authors: MD
authors: Caballero
authors: Alberto
authors: A
authors: Orfao
mesh_terms: D000328:Adult; D000368:Aged; D018941:Antigens, CD19 / Q000134:cerebrospinal fluid*; D014408:Biomarkers, Tumor / Q000134:cerebrospinal fluid*; D002051:Burkitt Lymphoma / Q000134:cerebrospinal fluid / Q000175:diagnosis / Q000276:immunology*; D016543:Central Nervous System Neoplasms / Q000134:cerebrospinal fluid / Q000175:diagnosis / Q000276:immunology*; D018572:Disease-Free Survival; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D053208:Kaplan-Meier Estimate; D016403:Lymphoma, Large B-Cell, Diffuse / Q000134:cerebrospinal fluid / Q000175:diagnosis / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012995:Solubility
chemical_list: D018941:Antigens, CD19; D014408:Biomarkers, Tumor