title: Accumulation and tolerance of lead in two contrasting ecotypes of Dianthus carthusianorum.
abstract: Dianthus carthusianorum is one of the dominant plant species colonising the Zn-Pb waste deposits in Bolesław, Southern Poland. It differs in terms of morphology and genetics from ecotypes inhabiting non-metal-polluted areas. The response of waste-heap (metallicolous, M) and reference (nonmetallicolous, NM) ecotypes of D. carthusianorum to Pb in hydroponics was investigated and compared in this study. The plants of the M ecotype were more tolerant to Pb than these of the NM ecotype in spite of accumulation of higher concentrations of Pb. In both ecotypes, about 70-78% of Pb was retained in roots. In non Pb-treated plants, a higher glutathione (GSH) level was found in the M ecotype. After the Pb exposure, the GSH level decreased and was similar in both ecotypes. Lead treatment induced synthesis of phytochelatins (PCs) only in the plant roots, with significantly higher concentrations thereof detected in the NM ecotype. Malate and citrate concentrations were higher in the M ecotype; however, they did not change significantly upon any Pb treatment in either ecotype. The results indicated that neither PCs nor organic acids were responsible for the enhanced Pb tolerance of the waste-heap plants.
authors: Department of Plant Physiology, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland. Electronic address: mwojcik@umcs.pl.
authors: Małgorzata
authors: M
authors: Wójcik
authors: Department of Plant Physiology, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland. Electronic address: anna.tukiendorf@umcs.pl.
authors: Anna
authors: A
authors: Tukiendorf
mesh_terms: D001692:Biological Transport; D019343:Citric Acid / Q000378:metabolism; D029749:Dianthus / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D060146:Ecotype; D005978:Glutathione / Q000378:metabolism; D007854:Lead / Q000378:metabolism* / Q000633:toxicity*; D008293:Malates / Q000378:metabolism; D054811:Phytochelatins / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000502:physiology; D012989:Soil Pollutants / Q000378:metabolism* / Q000633:toxicity*; D015032:Zinc / Q000378:metabolism
chemical_list: D008293:Malates; D012989:Soil Pollutants; D019343:Citric Acid; D007854:Lead; C030298:malic acid; D054811:Phytochelatins; D005978:Glutathione; D015032:Zinc
keywords: Caryophyllaceae; Dianthus carthusianorum; Glutathione; Lead tolerance; Organic acids; Phytochelatin