title: Human B cell immortalization for monoclonal antibody production.
abstract: Infection of primary B lymphocytes with Epstein-Barr virus gives rise to growth-transformed and immortalized lymphoblastoid cell lines (LCL) in vitro. Among their many applications is the use of LCL to present antigens in a variety of immunologic assays and to generate human monoclonal antibodies. This chapter describes a method to generate LCL from donor peripheral blood with rapid immortalization and cryopreservation times.
authors: Pediatric Infectious Diseases, Department of Pediatrics, Stony Brook University School of Medicine, Stony Brook, NY, USA.
authors: Joyce
authors: J
authors: Hui-Yuen
authors: Siva
authors: S
authors: Koganti
authors: Sumita
authors: S
authors: Bhaduri-McIntosh
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D000917:Antibody Formation / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D006801:Humans
chemical_list: D000911:Antibodies, Monoclonal