title: Cell adhesion as a novel approach to determining the cellular binding motif on the severe acute respiratory syndrome coronavirus spike protein.
abstract: Emerging life threatening pathogens such as severe acute aspiratory syndrome-coronavirus (SARS-CoV), avian-origin influenzas H7N9, and the Middle East respiratory syndrome coronavirus (MERS-CoV) have caused a high case-fatality rate and psychological effects on society and the economy. Therefore, a simple, rapid, and safe method to investigate a therapeutic approach against these pathogens is required. In this study, a simple, quick, and safe cell adhesion inhibition assay was developed to determine the potential cellular binding site on the SARS-CoV spike protein. Various synthetic peptides covering the potential binding site helped to minimize further the binding motif to 10-25 residues. Following analyses, 2 peptides spanning the 436-445 and 437-461 amino acids of the spike protein were identified as peptide inhibitor or peptide vaccine candidates against SARS-CoV.
authors: Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien, Taiwan; Institute of Medical Sciences, Tzu-Chi University, Hualien, Taiwan.
authors: Hsin-Hou
authors: HH
authors: Chang
authors: Institute of Medical Sciences, Tzu-Chi University, Hualien, Taiwan.
authors: Po-Kong
authors: PK
authors: Chen
authors: Institute of Medical Sciences, Tzu-Chi University, Hualien, Taiwan.
authors: Guan-Ling
authors: GL
authors: Lin
authors: The Department of Life Sciences, Tzu-Chi University, Hualien, Taiwan.
authors: Chun-Jen
authors: CJ
authors: Wang
authors: Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien, Taiwan.
authors: Chih-Hsien
authors: CH
authors: Liao
authors: Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien, Taiwan.
authors: Yu-Cheng
authors: YC
authors: Hsiao
authors: Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien, Taiwan.
authors: Jing-Hua
authors: JH
authors: Dong
authors: Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien, Taiwan; Institute of Medical Sciences, Tzu-Chi University, Hualien, Taiwan. Electronic address: dssun@mail.tcu.edu.tw.
authors: Der-Shan
authors: DS
authors: Sun
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification; D001665:Binding Sites; D002448:Cell Adhesion; D002460:Cell Line; D006801:Humans; D010455:Peptides / Q000302:isolation & purification; D011485:Protein Binding; D045473:Severe acute respiratory syndrome-related coronavirus / Q000502:physiology*; D064370:Spike Glycoprotein, Coronavirus / Q000378:metabolism*; D053585:Virus Attachment
chemical_list: D000998:Antiviral Agents; D010455:Peptides; D064370:Spike Glycoprotein, Coronavirus
keywords: Binding motif; SARS-CoV; Spike protein; Vero E6 cells