title: [Enrichment of haploid spermatids in mice by flow sorting].
abstract: OBJECTIVE
  To establish an effective method for haploid spermatid enrichment by Hoechst 33342 staining and flow sorting.
  
  
  METHODS
  Mouse testicular monoplast suspension was prepared by two-step enzyme digestion, and the cells were incubated in the medium containing Hoechst 33342 and Verapamil. Haploid spermatids were separated and enriched according to their DNA content by flow sorting. The gene expressions in the spermatids of several histone-modified enzymes, including the histone acetylases (HAT) and histone deacetylases (HDAC), were examined by RT-PCR and compared with that in the HAT-inhibitor curcumin-treated counterparts.
  
  
  RESULTS
  We successfully enriched the haploid spermatids with high purity and further purified the round and elongated spermatids. RT-PCR results indicated the specificity of the expression of the HAT gene in the spermatids, and that it was influenced by curcumin.
  
  
  CONCLUSIONS
  Flow sorting can efficiently improve the purity of haploid spermatid enrichment, which helps a lot to elucidate the mechanisms of spermiogenesis.
authors: Department of Histology and Embryology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China. zpxiaxy@shsmu.edu.cn
authors: Xiao-Yu
authors: XY
authors: Xia
authors: Department of Histology and Embryology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
authors: Xiao-Yu
authors: XY
authors: Xia
authors: Department of Biochemistry and Molecular and Cell Biology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
authors: Chen-Zhi
authors: CZ
authors: Guo
authors: Department of Histology and Embryology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
authors: Chen
authors: C
authors: Xu
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D006238:Haploidy; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D013087:Spermatids / Q000166:cytology*