title: Effect of in vitro exposure to lead chloride on semen quality and sperm DNA fragmentation.
abstract: Exposure to lead may cause changes in the male reproductive system. We evaluated the effect of lead chloride (PbCl2) in vitro on semen quality from 31 individuals. Samples were incubated at room temperature for two exposure times (4 h and 8 h) and with two concentrations of PbCl2 (15 μg/ml or 30 μg/ml). Results showed that PbCl2 significantly inhibited rapid progressive motility and caused an increase in the percentage of tail anomalies in both times and concentrations assessed, as well as a decrease in vitality in the group exposed to 30 μg/ml PbCl2. A significant increase in immotile sperm was also observed between the group control and the groups submitted to lead. Total motility and DNA fragmentation also showed a significant decrease and increase, respectively, after 4 h of incubation in the group exposed to 30 μg/ml and in both groups after 8 h of incubation. In conclusion, PbCl2 affected sperm parameters and DNA integrity, which are essential for male fertility.
authors: Department of Biology & CICECO,University of Aveiro,Campus Universitário de Santiago,3810-193,Aveiro,Portugal.
authors: M
authors: M
authors: Gomes
authors: Centre for Reproductive Genetics Prof. Alberto Barros,Av. do Bessa,240,1º Dto. Frente,4100-009 Porto,Portugal.
authors: A
authors: A
authors: Gonçalves
authors: Department of Microscopy,Laboratory of Histology and Embryology,Institute of Biomedical Sciences Abel Salazar,University of Porto,Rua Jorge Viterbo Ferreira,228,4050-313 Porto,E1,P2,R06,Porto,Portugal.
authors: E
authors: E
authors: Rocha
authors: Department of Microscopy,Laboratory of Cell Biology,Institute of Biomedical Sciences Abel Salazar,UMIB,University of Porto,Rua Jorge Viterbo Ferreira,228,4050-313 Porto,E1,P2,R07,Porto,Portugal.
authors: R
authors: R
authors: Sá
authors: Department of Microscopy,Laboratory of Cell Biology,Institute of Biomedical Sciences Abel Salazar,UMIB,University of Porto,Rua Jorge Viterbo Ferreira,228,4050-313 Porto,E1,P2,R07,Porto,Portugal.
authors: A
authors: A
authors: Alves
authors: Centre for Reproductive Genetics Prof. Alberto Barros,Av. do Bessa,240,1º Dto. Frente,4100-009 Porto,Portugal.
authors: J
authors: J
authors: Silva
authors: Centre for Reproductive Genetics Prof. Alberto Barros,Av. do Bessa,240,1º Dto. Frente,4100-009 Porto,Portugal.
authors: A
authors: A
authors: Barros
authors: Department of Biology & CICECO,University of Aveiro,Campus Universitário de Santiago,3810-193,Aveiro,Portugal.
authors: M L
authors: ML
authors: Pereira
authors: Department of Microscopy,Laboratory of Cell Biology (Director),Building 1,Floor 2,Room 07,Institute of Biomedical Sciences Abel Salazar (ICBAS),UMIB,University of Porto,Rua Jorge Viterbo Ferreira.
authors: M
authors: M
authors: Sousa
mesh_terms: D000328:Adult; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects*; D006801:Humans; D007854:Lead / Q000633:toxicity*; D008297:Male; D055101:Semen Analysis; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology
chemical_list: D007854:Lead; C029891:lead chloride
keywords: TUNEL assay