title: NG2-glia from pallial progenitors produce the largest clonal clusters of the brain: time frame of clonal generation in cortex and olfactory bulb.
abstract: NG2-glia are the most unknown population originating in the CNS. Despite their relative abundance in the brain, fundamental questions about their function, heterogeneity, and origin remain in debate. Particularly, it is still intriguing how these cells escaped from classical in vivo clonal analyses describing other neural types. Using StarTrack labeling in mouse brains, we found that NG2-glia are produced as immense clonal clusters whose number of cells is about one order of magnitude higher than in other neural types. Unexpectedly, this number remained low during embryonic and early postnatal stages, increasing during adulthood. In addition, we also demonstrated a pallial origin of a telencephalic NG2 population, which in the olfactory bulb is derived from local progenitors. Together, our results reveal an original ontogenic process that gives rise to the NG2-glia population and expands the previously established limits of development.
authors: Instituto Cajal, CSIC, Department of Molecular, Cellular, and Developmental Neurobiology, 28002 Madrid, Spain.
authors: Jorge
authors: J
authors: García-Marqués
authors: Raúl
authors: R
authors: Núñez-Llaves
authors: Laura
authors: L
authors: López-Mascaraque
mesh_terms: D000367:Age Factors; D000818:Animals; D000941:Antigens / Q000502:physiology*; D001921:Brain / Q000166:cytology / Q000254:growth & development; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation; D002540:Cerebral Cortex / Q000166:cytology* / Q000254:growth & development*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009457:Neuroglia / Q000502:physiology*; D009830:Olfactory Bulb / Q000166:cytology* / Q000254:growth & development*; D011247:Pregnancy; D011509:Proteoglycans / Q000502:physiology*; D013997:Time Factors; D000099216:Chondroitin Sulfate Proteoglycan 4
chemical_list: D000941:Antigens; D011509:Proteoglycans; D000099216:Chondroitin Sulfate Proteoglycan 4
keywords: NG2; StarTrack; clonal analysis; in utero electroporation; olfactory bulb; polydendrocytes