title: Accelerating proliferation of neural stem/progenitor cells in collagen sponges immobilized with engineered basic fibroblast growth factor for nervous system tissue engineering.
abstract: Neural stem/progenitor cells (NS/PCs) play a therapeutic role in nervous system diseases and contribute to functional recovery. However, their efficacy is limited as the majority of cells die post-transplantation. In this study, collagen sponges were utilized as carriers for NS/PCs. Basic fibroblast growth factor (bFGF), a mitogen for NS/PCs, was incorporated into the collagen sponges to stimulate NS/PC proliferation. However, the effect of native bFGF is limited because it diffuses into the culture medium and is lost following medium exchange. To overcome this problem, a collagen-binding polypeptide domain, which has high affinity to collagen, was fused with bFGF to sustain the exposure of NS/PCs within the collagen sponges to bFGF. The results indicated that the number of NS/PCs was significantly higher in collagen sponges incorporating engineered bFGF than in those with native bFGF or the PBS control after 7 days in culture. Here, we designed a natural biological neural scaffold consisting of collagen sponges, engineered bFGF, and NS/PCs. In addition to the effect of proliferated NS/PCs, the engineered bFGF retained in the natural biological neural scaffolds could have a direct effect on nervous system reconstruction. The two aspects of the natural biological neural scaffolds may produce synergistic effects, and so they represent a promising candidate for nervous system repair.
authors: The Affiliated Bayi Brain Hospital, Bayi Clinical College, Southern Medical University , No. 1838, North of Guangzhou Avenue, Guangzhou 510515, Guangdong, China.
authors: Fukai
authors: F
authors: Ma
authors: Zhifeng
authors: Z
authors: Xiao
authors: Bing
authors: B
authors: Chen
authors: Xianglin
authors: X
authors: Hou
authors: Jin
authors: J
authors: Han
authors: Yannan
authors: Y
authors: Zhao
authors: Jianwu
authors: J
authors: Dai
authors: Ruxiang
authors: R
authors: Xu
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000737:chemistry; D003470:Culture Media; D005346:Fibroblast Growth Factors / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009420:Nervous System / Q000187:drug effects*; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D003470:Culture Media; D005346:Fibroblast Growth Factors; D003094:Collagen