title: Paragonimus paishuihoensis metacercariae in freshwater crabs, Potamon lipkei, in Vientiane Province, Lao PDR.
abstract: Among Paragonimus species, P. paishuihoensis is one of the most mysterious and poorly understood species. Metacercariae are characterized by having a unique dendritically branched excretory bladder. However, the morphology of the adult worm remains unknown. To date, metacercariae of this species have been reported only in China and Thailand. In this study, we first found P. paishuihoensis metacercariae in freshwater crabs, Potamon lipkei, in Hinheub District, Vientiane, Lao PDR, with a prevalence of 77.7% and the average intensity of 10.3 (range 1-28) metacercariae per crab. The molecular data based on ITS2 and CO1 markers indicated that P. paishuihoensis from Laos and Thailand were almost completely identical and were close to members of the Paragonimus bangkokensis/Paragonimus harinasutai complex. Attempts to infect experimental animals (cats, dogs, and rats) with P. paishuihoensis were unsuccessful, suggesting that these animals might be unsuitable definitive hosts for the species. Further studies are necessary to elucidate the taxonomic status and life cycle of P. paishuihoensis.
authors: Department of Microbiology and Immunology, School of Medicine, Fukuoka University, Fukuoka, Japan.
authors: Shigehisa
authors: S
authors: Habe
authors: Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, Hanoi, Vietnam.
authors: Pham Ngoc
authors: PN
authors: Doanh
authors: Sigma Clinic, Fukuoka, Japan.
authors: Shinichiro
authors: S
authors: Yahiro
authors: Institut de la Francophonie pour la Médecine Tropicale, Vientiane, Lao PDR.
authors: Nanthasane
authors: N
authors: Vannavong
authors: Institut de la Francophonie pour la Médecine Tropicale, Vientiane, Lao PDR.
authors: Hubert
authors: H
authors: Barennes
authors: Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Basel, Switzerland.
authors: Peter
authors: P
authors: Odermatt
authors: Faculties of Medicine and Pharmacy, Limoges, France.
authors: Gilles
authors: G
authors: Dreyfuss
authors: Laboratory of Veterinary Parasitic Diseases, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
authors: Yoichiro
authors: Y
authors: Horii
authors: Research Affairs, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors: Yukifumi
authors: Y
authors: Nawa
mesh_terms: D000818:Animals; D003386:Brachyura / Q000469:parasitology*; D016000:Cluster Analysis; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D005618:Fresh Water; D007812:Laos; D058488:Metacercariae / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010238:Paragonimus / Q000302:isolation & purification*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015995:Prevalence; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D021903:DNA, Ribosomal Spacer; D003576:Electron Transport Complex IV
keywords: Lao PDR; Paragonimus paishuihoensis; Potamon lipkei; freshwater crab; metacercaria