title: Characterization of carbonic anhydrase from Turkish native "Gerze" chicken and influences of metal ions on enzyme activity.
abstract: Abstract Carbonic anhydrase was purified and characterized from erythrocytes of the Turkish native chicken, Gerze, for the first time. The enzyme was purified 57.65-fold with a yield of 52%, and a specific activity of 954.08 U/mg proteins having optimum pH at 8.0; optimum temperature at 30 °C; optimum ionic strength at 10 mM and stable pH at 8.0. The purified enzyme had apparent KM and Vmax values of 0.73 mM and 0.236 μmol × min(-1), respectively. Al(+3), Hg(+2), Cu(+2), Pb(+2), and Cd(+2) showed inhibitory effects on the enzyme. Pb(+2) exhibited the strongest inhibitory action. Cd(+2) and Hg(+2) were moderate inhibitor, whereas Al(+3) and Cu(+2) showed weaker actions. All tested metals inhibited the enzyme in competitive manner. Our findings indicate that these metals inhibit the chicken enzyme in a similar manner to other α-CAs from mammals investigated earlier, but susceptibility to various metals differ between the native chicken and other mammalian enzymes.
authors: Department of Agricultural Biotechnology, Faculty of Agriculture, Ondokuz Mayıs University , Samsun , Turkey and.
authors: Levent
authors: L
authors: Mercan
authors: Deniz
authors: D
authors: Ekinci
authors: Claudiu T
authors: CT
authors: Supuran
mesh_terms: D000535:Aluminum / Q000737:chemistry; D000818:Animals; D002104:Cadmium / Q000737:chemistry; D002257:Carbonic Anhydrase Inhibitors / Q000737:chemistry*; D002256:Carbonic Anhydrases / Q000737:chemistry* / Q000302:isolation & purification; D002412:Cations; D002645:Chickens / Q000378:metabolism*; D003300:Copper / Q000737:chemistry; D056727:Endangered Species; D004912:Erythrocytes / Q000737:chemistry / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007854:Lead / Q000737:chemistry; D008628:Mercury / Q000737:chemistry; D019216:Metals, Heavy / Q000737:chemistry*; D019565:Metals, Light / Q000737:chemistry*; D009994:Osmolar Concentration; D014421:Turkey
chemical_list: D002257:Carbonic Anhydrase Inhibitors; D002412:Cations; D019216:Metals, Heavy; D019565:Metals, Light; D002104:Cadmium; D007854:Lead; D003300:Copper; D000535:Aluminum; D002256:Carbonic Anhydrases; D008628:Mercury
keywords: Carbonic anhydrase; gerze; inhibition; metal