title: Triterpenes and Steroids from Euphorbia denticulata Lam. With Anti-Herpes Symplex Virus Activity.
abstract: In this research, dried acetone:chloroform extract of aerial parts of E. denticulata as one of the endemic plants to Iran, afforded a number of triterpenes and steroids including: betulin, 24-methylene-cycloart-3-ol, cycloart-23Z-ene-3β,25-diol, cycloart-23E-ene-3β,25- diol, ergosta-8,24-dien-3-ol (obtusifoliol) and beta-sitosterol which were reported for the first time from this plant. The structure of these compounds was elucidated by NMR and mass spectroscopic methods. The MTS assay was used to determine the toxicity and antiviral activity of betulin and (3β,23E)-cycloarta-23-ene-3,25-diol. Betulin showed anti-HSV-1 activity with EC50 value of 84.37±0.02 μg/mL, and toxicity on normal vero cells with CC50 value of 660.718±0.072 μg/mL. (3β,23E)-Cycloarta-23-ene-3,25-diol showed antiviral effect with EC50 value of 86.63±0.03 μg/mL, and toxicity with CC50 value of 1089.21±0.25 μg/mL. The results revealed that these two compounds have the antiviral activity far below the CC50 value with selectivity index (CC50/EC50) values of 7.83, and 12.57, respectively.
authors: Isfahan Pharmaceutical Sciences Research Center. Isfahan University of Medical Sciences, Isfahan, Iran.
authors: Sara
authors: S
authors: Shamsabadipour
authors: Department of Pharmacognosy, Isfahan Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan Iran.
authors: Mustafa
authors: M
authors: Ghanadian
authors: Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran.
authors: Hojjatollah
authors: H
authors: Saeedi
authors: Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran.
authors: Mohammad Reza
authors: MR
authors: Rahimnejad
authors: Microbiology, Islamic Azad University, Falavarjan Branch, Young Researchers and Elites Club, Isfahan, Iran.
authors: Marzieh
authors: M
authors: Mohammadi-Kamalabadi
authors: Phytochemistry Research Center and School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors: Seyed Majid
authors: SM
authors: Ayatollahi
authors: Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
authors: Loghman
authors: L
authors: Salimzadeh
keywords: Euphorboa denticulate; anti- HSV-1 activity; cycloartanes; steroids; toxicity; triterpenes