title: Comparison of operating strategies for increased biogas production from thin stillage.
abstract: The effect of increasing organic loading rate (OLR) and simultaneously decreasing hydraulic retention time (HRT) during anaerobic digestion of sulphur- and nitrogen-rich thin stillage was investigated during operation of continuously stirred tank laboratory reactors at two different temperatures. The operating strategies and substrate were set in order to mimic an existing full-scale commercial biogas plant in Sweden. The reactors were operated for 554-570 days with a substrate mixture of thin stillage and milled grain, resulting in high ammonium concentrations (>4.5gL(-1)). Initially, one reactor was operated at 38°C, as in the full-scale plant, while in the experimental reactor the temperature was raised to 44°C. Both reactors were then subjected to increasing OLR (from 3.2 to 6.0gVSL(-1)d(-1)) and simultaneously decreasing HRT (from 45 to 24 days) to evaluate the effects of these operational strategies on process stability, hydrogen sulphide levels and microbial composition. The results showed that operation at 44°C was the most successful strategy, resulting in up to 22% higher methane yield compared with the mesophilic reactor, despite higher free ammonia concentration. Furthermore, kinetic studies revealed higher biogas production rate at 44°C compared with 38°C, while the level of hydrogen sulphide was not affected. Quantitative PCR analysis of the microbiological population showed that methanogenic archaea and syntrophic acetate-oxidising bacteria had responded to the new process temperature while sulphate-reducing bacteria were only marginally affected by the temperature-change.
authors: Tekniska verken i Linköping AB (publ.), Department of Biogas R&D, Box 1500, SE-581 15 Linköping, Sweden; Department of Microbiology, BioCenter, Swedish University of Agricultural Sciences, Box 7025, SE-750 07 Uppsala, Sweden. Electronic address: jan.moestedt@tekniskaverken.se.
authors: Jan
authors: J
authors: Moestedt
authors: Tekniska verken i Linköping AB (publ.), Department of Biogas R&D, Box 1500, SE-581 15 Linköping, Sweden.
authors: Erik
authors: E
authors: Nordell
authors: Department of Microbiology, BioCenter, Swedish University of Agricultural Sciences, Box 7025, SE-750 07 Uppsala, Sweden. Electronic address: anna.schnurer@slu.se.
authors: Anna
authors: A
authors: Schnürer
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D001105:Archaea / Q000235:genetics; D001419:Bacteria / Q000235:genetics; D056804:Biofuels / Q000032:analysis* / Q000382:microbiology; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D006862:Hydrogen Sulfide / Q000378:metabolism*; D013548:Sweden; D013696:Temperature
chemical_list: D056804:Biofuels; D000641:Ammonia; D006862:Hydrogen Sulfide
keywords: Ammonium; Anaerobic digestion; Biogas; Hydrogen sulphide; Temperature; Thin stillage