title: Composition of microbial oral biofilms during maturation in young healthy adults.
abstract: In the present study we aimed to analyze the bacterial community structure of oral biofilms at different maturation stages in young healthy adults. Oral biofilms established on membrane filters were collected from 32 human subjects after 5 different maturation intervals (1, 3, 5, 9 and 14 days) and the respective phylogenetic diversity was analyzed by 16S rDNA amplicon sequencing. Our analyses revealed highly diverse entire colonization profiles, spread into 8 phyla/candidate divisions and in 15 different bacterial classes. A large inter-individual difference in the subjects' microbiota was observed, comprising 35% of the total variance, but lacking conspicuous general temporal trends in both alpha and beta diversity. We further obtained strong evidence that subjects can be categorized into three clusters based on three differently occurring and mutually exclusive species clusters.
authors: Institute for General Microbiology, Christian-Albrechts-University, Kiel, Germany.
authors: Daniela
authors: D
authors: Langfeldt
authors: Institute for General Microbiology, Christian-Albrechts-University, Kiel, Germany.
authors: Sven C
authors: SC
authors: Neulinger
authors: Department of Prosthetic Dentistry and Biomaterials Science, Hannover Medical School, Hannover, Germany.
authors: Wieland
authors: W
authors: Heuer
authors: Department of Conservative Dentistry, Periodontology and Preventive Dentistry, Hannover Medical School, Hannover, Germany.
authors: Ingmar
authors: I
authors: Staufenbiel
authors: Max-Planck Institute for Evolutionary Biology, Plön, Germany.
authors: Sven
authors: S
authors: Künzel
authors: Max-Planck Institute for Evolutionary Biology, Plön, Germany ; Institute for Experimental Medicine, Christian-Albrechts-University, Kiel, Germany.
authors: John F
authors: JF
authors: Baines
authors: Department of Prosthetic Dentistry and Biomaterials Science, Hannover Medical School, Hannover, Germany.
authors: Jörg
authors: J
authors: Eberhard
authors: Institute for General Microbiology, Christian-Albrechts-University, Kiel, Germany.
authors: Ruth A
authors: RA
authors: Schmitz
mesh_terms: D001419:Bacteria / Q000254:growth & development*; D018441:Biofilms / Q000254:growth & development*; D006262:Health; D006801:Humans; D009055:Mouth / Q000382:microbiology*; D013045:Species Specificity; D055815:Young Adult