title: ZSWIM1: a novel biomarker in T helper cell differentiation.
abstract: The effector memory CD4+ Th17 cells play critical roles in bacterial immunity and pathological inflammation in autoimmune conditions. ZSWIM1 is a gene encoding a protein of unknown function in leukocytes-but containing a zinc finger SWIM motif. In peripheral blood mononuclear cells, the expression of ZSWIM1 is highest in lymphocytes, and in particular shows greatest abundance in naive CD4+ T cells. Upon polarisation of naïve CD4+ T cells, ZSWIM1 expression is retained in Th17 cells but is selectively down regulated in Th1 cells. Similarly in in vitro expanded effector memory CD4+ T cells, ZSWIM1 was more abundant in Th17 cells compared to Th1 or Th17 polyfunctional (Th17pf) cells, which produce IL-17A and IFNγ. Although stimulation of cytokine production by PMA and ionomycin reduced ZSWIM1 expression, the relative differences in abundance between the cell types were maintained. The activation sensitive nature of ZSWIM1 expression suggests that it may play a novel role in the development or function of T helper cells.
authors: Centre for Biomedical Research, Burnet Institute, Melbourne 3004, VIC, Australia; Department of Pathology, University of Melbourne, Melbourne 3084, VIC, Australia. Electronic address: kko@burnet.edu.au.
authors: K Kerry
authors: KK
authors: Ko
authors: Centre for Biomedical Research, Burnet Institute, Melbourne 3004, VIC, Australia; Department of Pathology, University of Melbourne, Melbourne 3084, VIC, Australia; School of Public Health and Preventive Medicine, Monash University, Clayton 3168, VIC, Australia. Electronic address: maree.powell@monash.edu.
authors: Maree S
authors: MS
authors: Powell
authors: Centre for Biomedical Research, Burnet Institute, Melbourne 3004, VIC, Australia; Department of Pathology, University of Melbourne, Melbourne 3084, VIC, Australia; Department of Immunology, Monash University, Melbourne 3004, VIC, Australia. Electronic address: pmhogarth@burnet.edu.au.
authors: P Mark
authors: PM
authors: Hogarth
mesh_terms: D000595:Amino Acid Sequence; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002469:Cell Separation; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000276:immunology; D005786:Gene Expression Regulation; D006801:Humans; D007156:Immunologic Memory; D007371:Interferon-gamma / Q000096:biosynthesis; D020381:Interleukin-17 / Q000096:biosynthesis; D015759:Ionomycin / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008969:Molecular Sequence Data; D015398:Signal Transduction; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D018417:Th1 Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D058504:Th17 Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D016335:Zinc Fingers / Q000235:genetics*
chemical_list: D015415:Biomarkers; D004268:DNA-Binding Proteins; C508717:IL17A protein, human; D020381:Interleukin-17; C000595352:ZSWIM1 protein, human; D015759:Ionomycin; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate
keywords: IL-17A; Inflammation; SWIM domain; T helper cells; Th1 cells; Th17 cells