title: Preparation and characterization of genipin cross-linked porous chitosan-collagen-gelatin scaffolds using chitosan-CO2 solution.
abstract: Novel porous scaffolds composed of chitosan, collagen and gelatin were prepared by the multistep procedure involving final freeze-drying and characterized. To eliminate the need for residual acid removal from the material after drying, carbon dioxide saturation process was used for chitosan blend formulation. The use of CO2 for chitosan dissolution made the scaffold preparation process more reproducible and economically sustainable. Genipin was applied to stabilize the structure of the scaffolds and those crosslinked at a level of 7.3% exhibited a homogenous porous structure (33.1%), high swelling capacity (27.6g/g for wound exudate like medium; 62.5 g/g for water), and were stable under cyclic compression. The values of other investigated parameters: dissolution degree (30%), lysozyme-induced degradation (5% after 168 h), good antioxidant properties (DPPH, ABTS, Fe(2+) assays) and especially very low in vitro cytotoxicity against fibroblasts (103%, MTT assay), were highly advantageous for possible biomedical applications of the novel materials.
authors: Department of Pharmaceutical Technology and Biochemistry, Gdansk University of Technology, Gabriela Narutowicza Street 11/12, Gdansk 80-233, Poland. Electronic address: gregory.gorczyca@gmail.com.
authors: Grzegorz
authors: G
authors: Gorczyca
authors: Department of Chemistry, Technology and Biotechnology of Food, Gdansk University of Technology, Gabriela Narutowicza Street 11/12, Gdansk 80-233, Poland.
authors: Robert
authors: R
authors: Tylingo
authors: Department of Pharmaceutical Technology and Biochemistry, Gdansk University of Technology, Gabriela Narutowicza Street 11/12, Gdansk 80-233, Poland.
authors: Piotr
authors: P
authors: Szweda
authors: Department of Pharmaceutical Technology and Biochemistry, Gdansk University of Technology, Gabriela Narutowicza Street 11/12, Gdansk 80-233, Poland.
authors: Ewa
authors: E
authors: Augustin
authors: Department of Chemistry, Technology and Biotechnology of Food, Gdansk University of Technology, Gabriela Narutowicza Street 11/12, Gdansk 80-233, Poland.
authors: Maria
authors: M
authors: Sadowska
authors: Department of Pharmaceutical Technology and Biochemistry, Gdansk University of Technology, Gabriela Narutowicza Street 11/12, Gdansk 80-233, Poland.
authors: Sławomir
authors: S
authors: Milewski
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001672:Biocompatible Materials; D002245:Carbon Dioxide / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D039823:Iridoids / Q000737:chemistry*; D051379:Mice; D009113:Muramidase / Q000737:chemistry; D041681:NIH 3T3 Cells; D016062:Porosity; D012995:Solubility
chemical_list: D000975:Antioxidants; D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D039823:Iridoids; D002245:Carbon Dioxide; D005780:Gelatin; D003094:Collagen; D048271:Chitosan; C007834:genipin; D009113:Muramidase
keywords: Carbon dioxide; Chitosan; Collagen; Genipin; Porous scaffold