title: Rutin rich Emblica officinalis Geart. fruit extract ameliorates inflammation in the pancreas of rats subjected to alcohol and cerulein administration.
abstract: BACKGROUND
  The modulating effect of methanolic extract of Emblica officinalis (MEEO) on ethanol (EtOH)- and cerulein (Cer)-induced pancreatitis in rats was investigated in this study.
  
  
  METHODS
  Male albino Wistar rats were divided into four groups. Group 1 and 2 rats served as control and fed normal diet. Group 3 and 4 rats were fed isocalorically adjusted diet containing EtOH (36% of total calories) for 5 weeks and also subjected to intraperitoneal injection of Cer 20 µg/kg b.wt. thrice weekly for the last 3 weeks of the experimental period. In addition, group 2 and 4 rats received 200 mg/kg b.wt. of MEEO from 15th day till the experimental period. Serum levels of lipase (L), amylase (A), cytokines IL-1β, IL-18, caspase-1 and oxidative stress index (OSI) were determined. Levels of fecal trypsin, total collagen, caspase-1, myeloperoxidase (MPO), antioxidants and mRNA expression of caspase-1, IL-1β and IL-18 were determined in the pancreas.
  
  
  RESULTS
  HPLC analysis showed the presence of rutin in MEEO. We observed a significant elevation in serum L/A ratio, IL-1β, IL-18, caspase-1, OSI, collagen, MPO activity and the mRNA expression of IL-1β, IL-18 and caspase-1 and significant reduction in fecal trypsin and antioxidant status in EtOH- and Cer-administered rats. The inflammatory markers were found to be reduced and the antioxidant status of pancreas was maintained in MEEO-coadministered rats.
  
  
  CONCLUSIONS
  The rutin rich nature of E. officinalis can be claimed for its anti-inflammatory and pancreato protective effects.
authors: Ravikumar
authors: R
authors: Aruna
authors: Arumugam
authors: A
authors: Geetha
authors: Periyanayagam
authors: P
authors: Suguna
authors: Vijayashankar
authors: V
authors: Suganya
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D015415:Biomarkers / Q000378:metabolism; D002108:Ceruletide / Q000633:toxicity; D002851:Chromatography, High Pressure Liquid; D003094:Collagen / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D000431:Ethanol / Q000633:toxicity; D005638:Fruit; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010179:Pancreas / Q000737:chemistry; D010195:Pancreatitis / Q000139:chemically induced / Q000188:drug therapy*; D029842:Phyllanthus emblica; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000494:pharmacology*; D017208:Rats, Wistar; D012431:Rutin / Q000494:pharmacology*; D014357:Trypsin / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D015415:Biomarkers; D016207:Cytokines; D010936:Plant Extracts; D000431:Ethanol; D012431:Rutin; D002108:Ceruletide; D003094:Collagen; D014357:Trypsin