title: Identification of Sola l 4 as Bet v 1 homologous pathogenesis related-10 allergen in tomato fruits.
abstract: METHODS
  The aim was to investigate the potential contribution of a major birch pollen Bet v 1-homologous allergen to birch pollen-associated tomato fruit allergy.
  
  
  RESULTS
  Two isoforms of a Bet v 1-homologous protein (designated Sola l 4) from tomato fruit were identified by cDNA-cloning and produced as recombinant proteins. Allergen-specific IgE levels to tomato, birch pollen, Bet v 1, and Sola l 4 were determined in birch pollen allergic patients with allergy or tolerance to tomato. Sola l 4 was recognized in 76% of birch/tomato allergic patients, while tomato- and Bet v 1-specific IgE was detectable in 64% and 81% of sera. Almost all patients sensitized to Bet v 1 reacted with Sola l 4. Both Sola l 4 isoforms displayed allergenic potency and IgE-cross-reactivity with Bet v 1 as investigated by competitive ELISA and in vitro mediator release assay. Nevertheless, the reactivity pattern of patients' sera was diverse.
  
  
  CONCLUSIONS
  Sola l 4, a novel pathogenesis related-10 protein, qualifies as major allergen in tomato fruits. Data suggest Sola l 4 as class II allergen. IgE-testing using Sola l 4 showed low clinical specificity, but high sensitivity in tomato allergic patients and will further improve component-resolved allergy diagnosis.
authors: Paul-Ehrlich-Institut, Division of Allergology, Langen, Germany.
authors: Andrea
authors: A
authors: Wangorsch
authors: Annette
authors: A
authors: Jamin
authors: Kay
authors: K
authors: Foetisch
authors: Anna
authors: A
authors: Malczyk
authors: Andreas
authors: A
authors: Reuter
authors: Sabine
authors: S
authors: Vierecke
authors: Stefan
authors: S
authors: Schülke
authors: Detlef
authors: D
authors: Bartel
authors: Vera
authors: V
authors: Mahler
authors: Jonas
authors: J
authors: Lidholm
authors: Stefan
authors: S
authors: Vieths
authors: Stephan
authors: S
authors: Scheurer
mesh_terms: D000595:Amino Acid Sequence; D052179:Antigens, Plant / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D003001:Cloning, Molecular; D003429:Cross Reactions; D005512:Food Hypersensitivity / Q000276:immunology*; D005638:Fruit / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D018551:Solanum lycopersicum / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000276:immunology; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology
chemical_list: D052179:Antigens, Plant; D011994:Recombinant Proteins; C061312:Bet v 1 allergen, Betula; D007073:Immunoglobulin E
keywords: Bet v 1; Birch pollen allergy; Pathogenesis related-10 protein; Sola l 4; Tomato allergy