title: DSSylation, a novel protein modification targets proteins induced by oxidative stress, and facilitates their degradation in cells.
abstract: Timely removal of oxidatively damaged proteins is critical for cells exposed to oxidative stresses; however, cellular mechanism for clearing oxidized proteins is not clear. Our study reveals a novel type of protein modification that may play a role in targeting oxidized proteins and remove them. In this process, DSS1 (deleted in split hand/split foot 1), an evolutionally conserved small protein, is conjugated to proteins induced by oxidative stresses in vitro and in vivo, implying oxidized proteins are DSS1 clients. A subsequent ubiquitination targeting DSS1-protein adducts has been observed, suggesting the client proteins are degraded through the ubiquitin-proteasome pathway. The DSS1 attachment to its clients is evidenced to be an enzymatic process modulated by an unidentified ATPase. We name this novel protein modification as DSSylation, in which DSS1 plays as a modifier, whose attachment may render target proteins a signature leading to their subsequent ubiquitination, thereby recruits proteasome to degrade them.
authors: Medical Research Division, Regional Academic Health Center, Edinburg, TX, 78541, USA.
authors: Yinghao
authors: Y
authors: Zhang
authors: Fang-Mei
authors: FM
authors: Chang
authors: Jianjun
authors: J
authors: Huang
authors: Jacob J
authors: JJ
authors: Junco
authors: Shivani K
authors: SK
authors: Maffi
authors: Hannah I
authors: HI
authors: Pridgen
authors: Gabriel
authors: G
authors: Catano
authors: Hong
authors: H
authors: Dang
authors: Xiang
authors: X
authors: Ding
authors: Fuquan
authors: F
authors: Yang
authors: Dae Joon
authors: DJ
authors: Kim
authors: Thomas J
authors: TJ
authors: Slaga
authors: Rongqiao
authors: R
authors: He
authors: Sung-Jen
authors: SJ
authors: Wei
mesh_terms: D005609:Free Radicals / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000235:genetics*; D046988:Proteasome Endopeptidase Complex / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D046188:Protein Modification, Translational / Q000235:genetics*; D025801:Ubiquitin / Q000378:metabolism; D054875:Ubiquitination / Q000235:genetics*
chemical_list: D005609:Free Radicals; C484606:SEM1 protein, human; D025801:Ubiquitin; D046988:Proteasome Endopeptidase Complex