title: Ion exchange-high-performance liquid chromatography (IEX-HPLC).
abstract: The ion exchange-high-performance liquid chromatography is a high-throughput analytical method that allows to determine the charge profile of purified antibodies. Here, we describe the preparation of the samples, chromatographic conditions to be used (buffer preparation, salt gradient, quantity injected, flow rate, run time, column suitability, etc.), validity of the analysis, and integration of the chromatogram in order to calculate the proportion of the different isoforms.
authors: NovImmune S.A., Plan-Les-Ouates, Switzerland.
authors: Marie
authors: M
authors: Corbier
authors: Delphine
authors: D
authors: Schrag
authors: Sylvain
authors: S
authors: Raimondi
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange