title: Immunization, hybridoma generation, and selection for monoclonal antibody production.
abstract: Monoclonal antibodies (MAbs) produced by a single clone of cells with homogeneous binding specificity for an antigenic determinant have been used in diagnostics and therapeutics. Many new methods have been devised by scientists for making hybridomas and MAbs. The three major steps for producing MAbs are immunization, immortalization, and isolation. Here, we describe technical details of the three important steps for generating mouse hybridomas and MAbs.
authors: Diagnostics and Translational Research Center, Henry M. Jackson Foundation for the Advancement of Military Medicine, Gaithersburg, MD, USA.
authors: Hyung-Yong
authors: HY
authors: Kim
authors: Alexander
authors: A
authors: Stojadinovic
authors: Mina J
authors: MJ
authors: Izadjoo
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000378:metabolism; D000917:Antibody Formation / Q000276:immunology; D006825:Hybridomas / Q000276:immunology / Q000378:metabolism*; D051379:Mice
chemical_list: D000911:Antibodies, Monoclonal