title: Analysis of hepatic deiodinase 2 mRNA levels in natural fish lake populations exposed to different levels of putative thyroid disrupters.
abstract: Hepatic mRNA levels of the dio2 gene (deiodinase 2), implicated in thyroid hormone homeostasis, were analyzed in trout from six remote lakes in the Pyrenees (Spain) and the Tatra Mountains (Slovakia). Highest levels corresponded to fish from the two coldest lakes in Pyrenees, whereas relatively low levels were found in the Tatra lakes. These values correlated with the presence of highly-brominated polybrominated diphenyl ethers (PBDE) congeners in the muscle of the same animals, reflecting the distribution of these compounds across European mountain ranges. In contrast, cyp1a expression levels, diagnostic for the presence of dioxin-like pollutants, mirrored the distribution of semi-volatile organochlorine compounds, indicating the specificity of the two types of biological responses. Exposure to PDBEs is known to increase transcription of dio2 and other thyroid-related genes in laboratory experiments; we propose that our data reflects the same phenomenon in natural populations, driven by anthropogenic pollutants at the environmental concentrations.
authors: Institute of Environmental Assessment and Water Research (IDAEA-CSIC), Molecular and Cellular Biology, Jordi Girona, 18, 08034 Barcelona, Spain; Masaryk University, Faculty of Science, RECETOX, Kamenice 5/753, Brno CZ62500, Czech Republic.
authors: Sergio
authors: S
authors: Jarque
authors: Institute of Environmental Assessment and Water Research (IDAEA-CSIC), Molecular and Cellular Biology, Jordi Girona, 18, 08034 Barcelona, Spain.
authors: Carme
authors: C
authors: Bosch
authors: Institute of Environmental Assessment and Water Research (IDAEA-CSIC), Molecular and Cellular Biology, Jordi Girona, 18, 08034 Barcelona, Spain.
authors: Marta
authors: M
authors: Casado
authors: Institute of Environmental Assessment and Water Research (IDAEA-CSIC), Molecular and Cellular Biology, Jordi Girona, 18, 08034 Barcelona, Spain.
authors: Joan O
authors: JO
authors: Grimalt
authors: Institute of Environmental Assessment and Water Research (IDAEA-CSIC), Molecular and Cellular Biology, Jordi Girona, 18, 08034 Barcelona, Spain.
authors: Demetrio
authors: D
authors: Raldúa
authors: Institute of Environmental Assessment and Water Research (IDAEA-CSIC), Molecular and Cellular Biology, Jordi Girona, 18, 08034 Barcelona, Spain. Electronic address: bpcbmc@cid.csic.es.
authors: Benjamin
authors: B
authors: Piña
mesh_terms: D000818:Animals; D052244:Endocrine Disruptors / Q000378:metabolism / Q000633:toxicity; D004784:Environmental Monitoring / Q000379:methods*; D005399:Fishes / Q000378:metabolism*; D055768:Halogenated Diphenyl Ethers / Q000378:metabolism / Q000633:toxicity; D006843:Hydrocarbons, Chlorinated / Q000378:metabolism / Q000633:toxicity; D007453:Iodide Peroxidase / Q000235:genetics* / Q000378:metabolism; D060106:Lakes / Q000737:chemistry; D008099:Liver / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D018154:Slovakia; D013030:Spain; D013961:Thyroid Gland / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism* / Q000633:toxicity
chemical_list: D052244:Endocrine Disruptors; D055768:Halogenated Diphenyl Ethers; D006843:Hydrocarbons, Chlorinated; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D007453:Iodide Peroxidase
keywords: Airborne pollution; Aryl hydrocarbon Receptor; Persistent organic pollutants; Salmo trutta; qRT-PCR