title: Cotransplantation of bone marrow mononuclear cells and umbilical cord mesenchymal stem cells in avascular necrosis of the femoral head.
abstract: OBJECTIVE
  We sought to investigate the therapeutic effects of cotransplantation of autologous bone marrow mononuclear cells (BMMNCs) and allogeneic umbilical cord mesenchymal stem cells (UC-MSCs) on avascular necrosis of the femoral head (ANFH).
  
  
  METHODS
  In all, 30 patients (49 hips; 24 males and 6 females) with ANFH were enrolled. According to the system of the Association Research Circulation Osseous, there were 24 hips in phase II and 25 hips in phase Ⅲ. Blood supply to the femoral head was evaluated by using digital subtraction angiography. Generally, 60 to 80 mL of autologous BMMNCs and 30 to 50 mL of UC-MSCs were infused into the femoral head artery. Harris scores including pain and joint function were used to evaluate the effects before and 3, 6, 9, and 12 months after transplantation. Computed tomography and radiographs were performed before and 12 months after the treatment.
  
  
  RESULTS
  Clinical symptoms of pain and claudication were gradually improved. After the treatment, 93.3% (28/30), 86.7% (26/30), and 86.7% (26/30) of patients showed relief of hip pain, improvement of joint function, and extended walking distances, respectively. The Harris scores were increased significantly at 3, 6, and 12 months posttransplant compared with those pretransplant. In addition, the bone lesions in 89.7% of hips (44/49) were improved as showed on computed tomography after transplantation.
  
  
  CONCLUSIONS
  Cotransplantation of autologous BMMNCs and allogeneic UC-MSCs showed therapeutic effect on ANFH without severe adverse effects.
authors: Organ Transplant Institute, Fuzhou General Hospital, Xiamen University, Fuzhou, P.R. China.
authors: J
authors: J
authors: Cai
authors: Organ Transplant Institute, Fuzhou General Hospital, Xiamen University, Fuzhou, P.R. China.
authors: Z
authors: Z
authors: Wu
authors: Organ Transplant Institute, Fuzhou General Hospital, Xiamen University, Fuzhou, P.R. China.
authors: L
authors: L
authors: Huang
authors: Organ Transplant Institute, Fuzhou General Hospital, Xiamen University, Fuzhou, P.R. China.
authors: J
authors: J
authors: Chen
authors: Organ Transplant Institute, Fuzhou General Hospital, Xiamen University, Fuzhou, P.R. China.
authors: C
authors: C
authors: Wu
authors: Organ Transplant Institute, Fuzhou General Hospital, Xiamen University, Fuzhou, P.R. China.
authors: S
authors: S
authors: Wang
authors: Organ Transplant Institute, Fuzhou General Hospital, Xiamen University, Fuzhou, P.R. China.
authors: Z
authors: Z
authors: Deng
authors: Organ Transplant Institute, Fuzhou General Hospital, Xiamen University, Fuzhou, P.R. China.
authors: W
authors: W
authors: Wu
authors: Organ Transplant Institute, Fuzhou General Hospital, Xiamen University, Fuzhou, P.R. China.
authors: F
authors: F
authors: Luo
authors: Organ Transplant Institute, Fuzhou General Hospital, Xiamen University, Fuzhou, P.R. China. Electronic address: doctortjm@yahoo.com.
authors: J
authors: J
authors: Tan
mesh_terms: D000328:Adult; D001854:Bone Marrow Cells / Q000166:cytology; D016026:Bone Marrow Transplantation; D005260:Female; D005271:Femur Head Necrosis / Q000473:pathology* / Q000628:therapy*; D006801:Humans; D007231:Infant, Newborn; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008297:Male; D008875:Middle Aged; D009336:Necrosis / Q000097:blood*; D010865:Pilot Projects; D011859:Radiography; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology; D014057:Tomography, X-Ray Computed; D014184:Transplantation, Homologous; D014470:Umbilical Cord / Q000166:cytology; D055815:Young Adult