title: Transcoronary gradient of plasma microRNA 423-5p in heart failure: evidence of altered myocardial expression.
abstract: BACKGROUND
  Elevated plasmatic microRNAs (miRs) are observed in heart failure (HF). However, the cardiac origin of these miRs remains unclear.
  
  
  OBJECTIVE
  We calculated transcoronary gradients of miR-29b, miR-133a and miR-423-5p in 17 outpatients with stable systolic HF and in controls without structural cardiac disease.
  
  
  METHODS
  MicroRNAs were measured by quantitative real-time polymerase chain reaction.
  
  
  RESULTS
  Positive transcoronary miR gradients were observed in patients with HF but not in controls (p = 0.03). B-type natriuretic peptide (BNP) moderately correlated with the transcoronary gradients of miR-133a and miR-423-5p.
  
  
  CONCLUSIONS
  The difference in transcoronary gradients between HF outpatients and controls suggests that miR-423-5p has a cardiac origin. The positive correlation between miR-423-5p and BNP transcoronary gradients supports this hypothesis.
authors: Heart Failure and Cardiac Transplant Group, Cardiovascular Experimental and Molecular Laboratory, Division of Cardiology, Hospital de Clínicas de Porto Alegre , Porto Alegre , Brazil and.
authors: Livia A
authors: LA
authors: Goldraich
authors: Nidiane C
authors: NC
authors: Martinelli
authors: Ursula
authors: U
authors: Matte
authors: Carolina
authors: C
authors: Cohen
authors: Michael
authors: M
authors: Andrades
authors: Maurício
authors: M
authors: Pimentel
authors: Andréia
authors: A
authors: Biolo
authors: Nadine
authors: N
authors: Clausell
authors: Luis E
authors: LE
authors: Rohde
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D006333:Heart Failure / Q000097:blood*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D008875:Middle Aged; D009206:Myocardium / Q000378:metabolism*; D020097:Natriuretic Peptide, Brain / Q000097:blood; D010865:Pilot Projects
chemical_list: D015415:Biomarkers; C548900:MIRN423 microRNA, human; D035683:MicroRNAs; D020097:Natriuretic Peptide, Brain