title: Optimized extraction of polysaccharides from Cymbopogon citratus and its biological activities.
abstract: In this study the extraction of hot water soluble polysaccharides (HWSPs) from Cymbopogon citratus using hot water decoction was discussed. Response surface methodology (RSM) based on a three level, three variable central composite rotatable design (CCRD), was employed to obtain best possible combination of extraction time (X1: 30-180 min), extraction temperature (X2: 70-100 °C) and water to the raw material ratio (X3: 10-60) for maximum HWSPs extraction. The optimum extraction conditions were as follows: extraction time was around 113.81 min, extraction temperature at 99.66 °C and the ratio of water to raw material was 33.11 g/mL. Under these conditions, the experimental yield was 13.24±0.23%, which is well in close agreement with the value predicted by RSM model yield (13.19%). The basic characterization of HWSPs was determined by using the FTIR. These preliminary in vitro biological studies indicated that lemongrass polysaccharides were useful for anticancer therapy.
authors: Proteomics & Molecular Cell Physiology Lab (PMCPL), Department of Zoology, Bharathiar University, Coimbatore, TN, India; Department of Virology, King Institute of Preventive Medicine and Research, Chennai, TN, India. Electronic address: thangam1985@yahoo.co.in.
authors: Ramar
authors: R
authors: Thangam
authors: Department of Botany, Annamalai University, Annamalai Nagar, TN, India.
authors: Veeraperumal
authors: V
authors: Suresh
authors: Proteomics & Molecular Cell Physiology Lab (PMCPL), Department of Zoology, Bharathiar University, Coimbatore, TN, India; Department of Zoology, Periyar University, Salem, TN, India. Electronic address: skperiyaruniv@gmail.com.
authors: Soundarapandian
authors: S
authors: Kannan
mesh_terms: D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005591:Chemical Fractionation / Q000379:methods*; D031743:Cymbopogon / Q000737:chemistry*; D006358:Hot Temperature; D006801:Humans; D008962:Models, Theoretical; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D011134:Polysaccharides; D014867:Water
keywords: Antioxidant and anticancer effects; Cymbopogon citratus (lemongrass); Optimization; Polysaccharides (HWSPs); Response surface methodology