title: Serotonin-2C receptor involved serotonin-induced Ca²⁺ mobilisations in neuronal progenitors and neurons in rat suprachiasmatic nucleus.
abstract: The hypothalamic suprachiasmatic nucleus (SCN), the central circadian pacemaker in mammals, undergoes serotonergic regulation, but the underlying mechanisms remain obscure. Here, we generated a subclone of an SCN progenitor cell line expressing Ca(2+) sensors (SCN2.2YC) and compared its 5-HT receptor signalling with that of rat SCN neurons in brain slices. SCN2.2YC cells expressed 5-HT1A/2A/2B/2C, but not 5A/7, while all six subtypes were expressed in SCN tissues. High K(+) or 5-HT increased cytosolic Ca(2+) in SCN2.2YC cells. The 5-HT responses were inhibited by ritanserin and SB-221284, but resistant to WAY-100635 and RS-127445, suggesting predominant involvement of 5-HT2C for Ca(2+) mobilisations. Consistently, Ca(2+) imaging and voltage-clamp electrophysiology using rat SCN slices demonstrated post-synaptic 5-HT2C expression. Because 5-HT2C expression was postnatally increased in the SCN and 5-HT-induced Ca(2+) mobilisations were amplified in differentiated SCN2.2YC cells and developed SCN neurons, we suggest that this signalling development occurs in accordance with central clock maturations.
authors: Graduate School of Innovative Life Science, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan.
authors: Kouhei
authors: K
authors: Takeuchi
authors: Graduate School of Innovative Life Science, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan.
authors: Shahid
authors: S
authors: Mohammad
authors: Graduate School of Innovative Life Science, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan.
authors: Tomoya
authors: T
authors: Ozaki
authors: Graduate School of Innovative Life Science, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan.
authors: Eri
authors: E
authors: Morioka
authors: Graduate School of Science and Engineering, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan.
authors: Kaori
authors: K
authors: Kawaguchi
authors: 1] Graduate School of Innovative Life Science, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan [2] Graduate School of Science and Engineering, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan.
authors: Juhyon
authors: J
authors: Kim
authors: Center for Cell-dynamics and Department of Physics, Korea University, Anam-Dong 5-1, Sungbuk-Gu, Seoul 136-713, Korea.
authors: Byeongha
authors: B
authors: Jeong
authors: Center for Cell-dynamics and Department of Physics, Korea University, Anam-Dong 5-1, Sungbuk-Gu, Seoul 136-713, Korea.
authors: Jin Hee
authors: JH
authors: Hong
authors: Center for Cell-dynamics and Department of Physics, Korea University, Anam-Dong 5-1, Sungbuk-Gu, Seoul 136-713, Korea.
authors: Kyoung J
authors: KJ
authors: Lee
authors: 1] Graduate School of Innovative Life Science, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan [2] Graduate School of Science and Engineering, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan.
authors: Masayuki
authors: M
authors: Ikeda
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D066298:In Vitro Techniques; D007211:Indoles / Q000494:pharmacology; D008297:Male; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018408:Patch-Clamp Techniques; D010879:Piperazines / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D044405:Receptor, Serotonin, 5-HT2C / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016713:Ritanserin / Q000494:pharmacology; D012701:Serotonin / Q000494:pharmacology*; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013493:Suprachiasmatic Nucleus / Q000166:cytology; D059467:Transcriptome
chemical_list: D007211:Indoles; D010879:Piperazines; D011725:Pyridines; D011743:Pyrimidines; D044405:Receptor, Serotonin, 5-HT2C; C112099:SB 221284; C121466:2-amino-4-(4-fluoronaphth-1-yl)-6-isopropylpyrimidine; D016713:Ritanserin; D012701:Serotonin; C090413:N-(2-(4-(2-methoxyphenyl)-1-piperazinyl)ethyl)-N-(2-pyridinyl)cyclohexanecarboxamide; D002118:Calcium