title: Characterization of human papillomavirus genotypes and HPV-16 physical status in cervical neoplasias of women from northern Portugal.
abstract: OBJECTIVE
  To determine human papillomavirus (HPV) genotypes and the physical status of HPV-16 DNA among women from northern Portugal with cervical lesions.
  
  
  METHODS
  The present retrospective study included samples of cervical exfoliated cells from 88 women (median age 42.0±13.1 years) who attended the Gynecology Service at the Portuguese Institute of Oncology in Porto during 2010. After DNA extraction, HPV genotyping was performed by polymerase chain reaction (PCR) followed by restriction fragment length polymorphism analysis using the MY09/MY11 primers. The physical status of HPV-16 was determined by real-time PCR.
  
  
  RESULTS
  Overall, 69.3% of the samples tested positive. The prevalence of HPV infection was 38.5% in normal samples, 57.7% in cervicitis samples, and 87.2% in all cervical lesions including invasive cancers. Sixteen genotypes were detected, the most prevalent ones being HPV-16 (42.9%), HPV-31 (12.2%), and HPV-58 (10.2%); HPV-18 was rare. The overall prevalence of HPV-16 integration was 31.6%. The physical status of HPV-16 did not differ significantly by histology.
  
  
  CONCLUSIONS
  The most frequent genotypes were HPV-16, -31, and -58. Integration of HPV-16 DNA seemed to be an early event in cervical carcinogenesis. Further studies are required to clarify the value of viral integration as a prognostic marker.
authors: Virology Service, Portuguese Institute of Oncology, Porto, Portugal; Molecular Oncology Group, Portuguese Institute of Oncology, Porto, Portugal; Faculty of Medicine, University of Porto, Porto, Portugal; Research Department, Portuguese League Against Cancer (Liga Portuguesa Contra o Cancro - Núcleo Regional do Norte), Porto, Portugal.
authors: Joana
authors: J
authors: Ribeiro
authors: Virology Service, Portuguese Institute of Oncology, Porto, Portugal.
authors: Dulce
authors: D
authors: Teixeira
authors: Virology Service, Portuguese Institute of Oncology, Porto, Portugal; Molecular Oncology Group, Portuguese Institute of Oncology, Porto, Portugal.
authors: Joana
authors: J
authors: Marinho-Dias
authors: Department of Pathology, Portuguese Institute of Oncology, Porto, Portugal.
authors: Paula
authors: P
authors: Monteiro
authors: Department of Pathology, Portuguese Institute of Oncology, Porto, Portugal.
authors: Joana
authors: J
authors: Loureiro
authors: Virology Service, Portuguese Institute of Oncology, Porto, Portugal.
authors: Inês
authors: I
authors: Baldaque
authors: Virology Service, Portuguese Institute of Oncology, Porto, Portugal; Molecular Oncology Group, Portuguese Institute of Oncology, Porto, Portugal; Research Department, Portuguese League Against Cancer (Liga Portuguesa Contra o Cancro - Núcleo Regional do Norte), Porto, Portugal.
authors: Rui
authors: R
authors: Medeiros
authors: Virology Service, Portuguese Institute of Oncology, Porto, Portugal; Molecular Oncology Group, Portuguese Institute of Oncology, Porto, Portugal. Electronic address: hugomls@gmail.com.
authors: Hugo
authors: H
authors: Sousa
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005838:Genotype; D052162:Human papillomavirus 16 / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000821:virology*; D011174:Portugal; D012189:Retrospective Studies; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000821:virology*; D002575:Uterine Cervicitis / Q000473:pathology / Q000821:virology; D016662:Virus Integration; D002578:Uterine Cervical Dysplasia / Q000473:pathology / Q000821:virology*
chemical_list: D004279:DNA, Viral
keywords: Cervical cancer; HPV-16; Human papillomavirus genotypes; Integration