title: [Effect of Bushen Tiaojing Recipe and Xiaoyao Pill on adenohypophysis and ovary in androgen-induced sterile rats: a comparative study].
abstract: OBJECTIVE
  To observe the effect of Bushen Tiaojing Recipe (BTR) and Xiaoyao Pill (XYP) on the morphology and sex hormones secretion of adenohypophysis and ovaries in androgen-induced sterile rats (ASR).
  
  
  METHODS
  Fifty 9-day old SD female rats randomly recruited from total 60 rats were subcutaneously injected with testosterone propionate to establish the ASR model. And the rest 10 rats were recruited as the normal group. Thirty successfully modeled rats were recruited and randomly divided into the model group, the BTR group (administered with BTR suspension), and the XYP group (administered with XYP suspension), 10 in each group. Five weeks later, rats were decapitated in the proestrus. Serum levels of estradiol (E2), progesterone (P), testosterone (T), follicle-stimulating hormone (FSH), and luteinizing hormone (LH) were detected by radioimmunoassay. The morphologies of adenohypophysis and ovary were observed after HE staining.
  
  
  RESULTS
  Compared with the normal group, serum E2 and T levels increased, while FSH and LH levels decreased in the model group (all P < 0.01). The morphology of adenohypophysis and ovary was abnormal in the model group. Compared with the model group, serum E2 and T levels decreased, while FSH and LH levels increased in the BTR group and the XYP group (P < 0.05, P < 0.01). Besides, E2 and T levels in the BTR group and FSH levels in the XYP group restored to normal (all P > 0.05). The damaged structure of adenohypophysis and ovary got restored to different degrees.
  
  
  CONCLUSIONS
  BTR and XYP both could improve ovulation failure.
authors: College of Traditional Chinese Medicine, Hebei United University, Hebei 063000, China.
authors: Ding-Jie
authors: DJ
authors: Xu
authors: Department of Integrative Medical Gynecology, Heibei Medical University, Shijiazhuang 050091, China.
authors: Li-Wen
authors: LW
authors: Hong
authors: Experimental Research Center, Hebei United University, Hebei 063000, China.
authors: Hong
authors: H
authors: Xu
authors: Department of Integrative Medical Gynecology, Heibei Medical University, Shijiazhuang 050091, China.
authors: Hong-Min
authors: HM
authors: Yang
authors: Department of Integrative Medical Gynecology, Heibei Medical University, Shijiazhuang 050091, China.
authors: Man-Fang
authors: MF
authors: Liu
authors: Department of Integrative Medical Gynecology, Heibei Medical University, Shijiazhuang 050091, China.
authors: Hui-Lan
authors: HL
authors: Du
mesh_terms: D000728:Androgens / Q000009:adverse effects; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004958:Estradiol / Q000097:blood; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D007247:Infertility, Female / Q000139:chemically induced / Q000188:drug therapy; D007986:Luteinizing Hormone / Q000097:blood; D010053:Ovary / Q000187:drug effects*; D010060:Ovulation; D010903:Pituitary Gland, Anterior / Q000187:drug effects*; D011374:Progesterone / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D013739:Testosterone / Q000097:blood
chemical_list: D000728:Androgens; D004365:Drugs, Chinese Herbal; C516449:xiaoyao; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone