title: Layer-by-layer assembly of epidermal growth factors on polyurethane films for wound closure.
abstract: To facilitate the healing of chronic wounds, growth factors such as epidermal growth factor need to be safely encapsulated for their sustained and effective delivery to the wound bed. Using a layer-by-layer assembly technique, epidermal growth factor is successfully encapsulated on the surface of poly(acrylic acid)-modified polyurethane film. The amount of encapsulated epidermal growth factor is controlled by adjusting the number of chitosan/epidermal growth factor bilayers. A controlled release of epidermal growth factor from the surface of polyurethane film for a period of five days is achieved with well-retained bioactivity (over 90%) as evidenced by a cell proliferation assay. In an in vitro cellular wounding assay, the cell gap covered with the epidermal growth factor-loaded polyurethane film closes at a rate more than twice as fast as that covered with a control polyurethane film. Fluorescent staining of F-actin reveals that the released epidermal growth factor induces differences in cytoskeletal organization, suggesting that stimulated cell migration also contributes to the close of the cell gap.
authors: Department of Textile Sciences, Faculty of Human Ecology, University of Manitoba, Winnipeg, Manitoba, Canada.
authors: Abhilash
authors: A
authors: Kulkarni
authors: Faculty of Health Disciplines, Athabasca University, Athabasca, Alberta, Canada.
authors: William
authors: W
authors: Diehl-Jones
authors: Department of Chemistry, Faculty of Science, University of Manitoba, Winnipeg, Manitoba, Canada.
authors: Sadegh
authors: S
authors: Ghanbar
authors: Department of Textile Sciences, Faculty of Human Ecology, University of Manitoba, Winnipeg, Manitoba, Canada Department of Chemistry, Faculty of Science, University of Manitoba, Winnipeg, Manitoba, Canada Song.Liu@umanitoba.ca.
authors: Song
authors: S
authors: Liu
keywords: Delivery of EGF; LbL assembly; PU film; wound healing