title: Evaluation of the stability and antimicrobial activity of an ethanolic extract of Libidibia ferrea.
abstract: Biofilm is a dense, whitish, noncalcified aggregate of bacteria, with desquamated epithelial cells and food debris creating conditions for an imbalance of resident oral microflora and favoring the destruction of hard and soft tissues by development of caries and gingivitis. The aim of this study was to obtain and characterize an extract of Libidibia ferrea, ex Caesalpinia ferrea L. and to evaluate its feasibility for formulation as a mouthwash, according to current legislation. For this purpose, pH, sedimentation, density, and stability were evaluated, along with microbiological testing of the extract. The microbiological test was used to verify the presence of Staphylococcus aureus, Pseudomonas aeruginosa, fungi, yeasts, coliforms, and minimum inhibitory concentrations of Streptococcus mutans and Streptococcus oralis strains. Characterization, microbiological evaluation, and minimum inhibitory concentration results were tabulated and described using descriptive statistics. The L. ferrea extract showed stable characteristics, product quality, and antibacterial activity against the microorganisms tested irrespective of experimental time intervals. According to these results, it can be concluded that formulation of a mouthwash containing L. ferrea extract to control biofilm is feasible, but further studies are needed.
authors: School of Dentistry, Federal University of Amazonas, Manaus, Amazonas, Brazil.
authors: Raquel
authors: R
authors: de Oliveira Marreiro
authors: School of Dentistry, Federal University of Amazonas, Manaus, Amazonas, Brazil.
authors: Maria Fulgência Costa Lima
authors: MF
authors: Bandeira
authors: School of Pharmacy, Federal University of Amazonas, Manaus, Amazonas, Brazil.
authors: Tatiane Pereira
authors: TP
authors: de Souza
authors: School of Dentistry, Federal University of Amazonas, Manaus, Amazonas, Brazil.
authors: Mailza Costa
authors: MC
authors: de Almeida
authors: School of Dentistry, Federal University of Amazonas, Manaus, Amazonas, Brazil.
authors: Katiana
authors: K
authors: Bendaham
authors: School of Dentistry, Federal University of Amazonas, Manaus, Amazonas, Brazil.
authors: Gisely Naura
authors: GN
authors: Venâncio
authors: School of Pharmacy, Federal University of Amazonas, Manaus, Amazonas, Brazil.
authors: Isis Costa
authors: IC
authors: Rodrigues
authors: School of Dentistry, Federal University of Amazonas, Manaus, Amazonas, Brazil.
authors: Cristiane Nagai
authors: CN
authors: Coelho
authors: School of Dentistry, Federal University of Amazonas, Manaus, Amazonas, Brazil.
authors: Patrícia Sâmea Lêdo Lima
authors: PS
authors: Milério
authors: School of Dentistry, Federal University of Amazonas, Manaus, Amazonas, Brazil.
authors: Glauber Palma
authors: GP
authors: de Oliveira
authors: School of Dentistry, Federal University of Amazonas, Manaus, Amazonas, Brazil.
authors: Nikeila Chacon
authors: NC
authors: de Oliveira Conde
keywords: biofilm; extract; quality control