title: Poly (ADP-ribose) polymerase 3 (PARP3), a potential repressor of telomerase activity.
abstract: BACKGROUND
  Considering previous result in Non-Small Cell Lung Cancer (NSCLC), we investigated in human cancer cells the role of PARP3 in the regulation of telomerase activity.
  
  
  METHODS
  We selected A549 (lung adenocarcinoma cell line) and Saos-2 (osteosarcoma cell line), with high and low telomerase activity levels, respectively. The first one was transfected using a plasmid construction containing a PARP3 sequence, whereas the Saos-2 cells were submitted to shRNA transfection to get PARP3 depletion. PARP3 expression on both cell systems was evaluated by real-time quantitative PCR and PARP3 protein levels, by Western-blot. Telomerase activity was determined by TRAP assay.
  
  
  RESULTS
  In A549 cells, after PARP3 transient transfection, data obtained indicated that twenty-four hours after transfection, up to 100-fold increased gene expression levels were found in the transfected cells with pcDNA/GW-53/PARP3 in comparison to transfected cells with the empty vector. Moreover, 48 hours post-transfection, telomerase activity decreased around 33%, and around 27%, 96 hours post-transfection. Telomerase activity average ratio was 0.67 ± 0.05, and 0.73 ± 0.06, respectively, with significant differences. In Saos-2 cells, after shRNA-mediated PARP3 silencing, a 2.3-fold increase in telomerase activity was detected in relation to the control.
  
  
  CONCLUSIONS
  Our data indicated that, at least in some cancer cells, repression of PARP3 could be responsible for an increased telomerase activity, this fact contributing to telomere maintenance and, therefore, avoiding genome instability.
authors: Tamara
authors: T
authors: Fernández-Marcelo
authors: Cristina
authors: C
authors: Frías
authors: Irene
authors: I
authors: Pascua
authors: Carmen
authors: C
authors: de Juan
authors: Jacqueline
authors: J
authors: Head
authors: Ana
authors: A
authors: Gómez
authors: Florentino
authors: F
authors: Hernando
authors: Jose-Ramon
authors: JR
authors: Jarabo
authors: Eduardo
authors: E
authors: Díaz-Rubio
authors: Antonio-Jose
authors: AJ
authors: Torres
authors: Michèle
authors: M
authors: Rouleau
authors: Manuel
authors: M
authors: Benito
authors: Departamento de Bioquímica y Biología Molecular II, Facultad de Farmacia, Universidad Complutense y Servicios de, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), Madrid 28040, Spain. insepi@ucm.es.
authors: Pilar
authors: P
authors: Iniesta
mesh_terms: D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D015971:Gene Expression Regulation, Enzymologic; D055785:Gene Knockdown Techniques; D006801:Humans; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D019098:Telomerase / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C480979:PARP3 protein, human; D011065:Poly(ADP-ribose) Polymerases; D019098:Telomerase