title: Enhanced production of 2,3-butanediol by overexpressing acetolactate synthase and acetoin reductase in Klebsiella pneumoniae.
abstract: Mutants with overexpression of α-acetolactate synthase (ALS), α-acetolactate decarboxylase, and acetoin reductase (AR), either individually or in combination, were constructed to improve 2,3-butanediol (2,3-BD) production in Klebsiella pneumoniae. The recombinant strains were characterized in terms of the enzyme activity, 2,3-BD yield, and expression levels. The recombinant K. pneumoniae strain (KG-rs) that overexpressed both ALS and AR showed an improved 2,3-BD yield. When cultured in the media with five different carbon sources (glucose, galactose, fructose, sucrose, and lactose), the mutant exhibited higher 2,3-BD productivity and production than the parental strain in all the tested carbon sources except for lactose. The 2,3-BD production of KG-rs in a batch fermentation with glucose as the carbon source was 12% higher than that of the parental strain.
authors: School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, People's Republic of China; Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin, People's Republic of China; Tianjin Industrial Microbiology Key Laboratory, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, People's Republic of China.
authors: Xue-Wu
authors: XW
authors: Guo
authors: Yun-Hui
authors: YH
authors: Zhang
authors: Chun-Hong
authors: CH
authors: Cao
authors: Tong
authors: T
authors: Shen
authors: Ming-Yue
authors: MY
authors: Wu
authors: Ye-Fu
authors: YF
authors: Chen
authors: Cui-Ying
authors: CY
authors: Zhang
authors: Dong-Guang
authors: DG
authors: Xiao
mesh_terms: D000095:Acetolactate Synthase / Q000096:biosynthesis* / Q000235:genetics; D000429:Alcohol Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics; D002072:Butylene Glycols / Q000138:chemical synthesis* / Q000737:chemistry; D002244:Carbon / Q000378:metabolism*; D005285:Fermentation; D015964:Gene Expression Regulation, Bacterial; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics; D007773:Lactates / Q000737:chemistry; D009154:Mutation
chemical_list: D002072:Butylene Glycols; D007773:Lactates; C026978:2,3-butylene glycol; C006359:alpha-acetolactate; D002244:Carbon; D000429:Alcohol Oxidoreductases; C019801:butanediol dehydrogenase; D000095:Acetolactate Synthase
keywords: 2,3-BD; Klebsiella pneumoniae; acetoin reductase; acetolactate decarboxylase; acetolactate synthase