title: Target-responsive, DNA nanostructure-based E-DNA sensor for microRNA analysis.
abstract: Because of the short size and low abundance of microRNAs, it is challenging to develop fast, inexpensive, and simple biosensors to detect them. In this work, we have demonstrated a new generation (the third generation) of E-DNA sensor for the sensitive and specific detection of microRNAs. Our third generation of E-DNA sensor can sensitively detect microRNA target (microRNA-141) as low as 1 fM. The excellent specificity has been demonstrated by its differential ability to the highly similar microRNA analogues. In our design, the use of DNA tetrahedron ensures the stem-loop structure in well controlled density with improved reactivity. The regulation of the thermodynamic stability of the stem-loop structure decreases the background signal and increases the specificity as well. The enzymes attached bring the electrocatalytic signal to amplify the detection. The combination of these effects improves the sensitivity of the E-DNA sensor and makes it suitable to the microRNA detection. Finally, our third generation of E-DNA sensor is generalizable to the detection of other micro RNA targets (for example, microRNA-21).
authors: Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai, China 201800.
authors: Meihua
authors: M
authors: Lin
authors: Yanli
authors: Y
authors: Wen
authors: Lanying
authors: L
authors: Li
authors: Hao
authors: H
authors: Pei
authors: Gang
authors: G
authors: Liu
authors: Haiyun
authors: H
authors: Song
authors: Xiaolei
authors: X
authors: Zuo
authors: Chunhai
authors: C
authors: Fan
authors: Qing
authors: Q
authors: Huang
mesh_terms: D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry*; D035683:MicroRNAs / Q000032:analysis*; D049329:Nanostructures; D009690:Nucleic Acid Conformation
chemical_list: D035683:MicroRNAs; D004247:DNA