title: MicroRNA-23a promotes neuroblastoma cell metastasis by targeting CDH1.
abstract: CDH1 inactivation is important in tumor metastasis. In the present study, it was suggested that the mRNA and protein levels of CDH1 decreased in metastatic neuroblastoma (NB) tissues compared with those in primary NB tissues. The aim of the study was to explore the regulatory mechanisms of CDH1 downregulation in metastatic NB. MicroRNAs are small non-coding RNAs (~22 nt in length) that negatively regulate target mRNAs and are involved in various cancer-related processes, including metastasis. In the current study, miR-23a was shown to be upregulated in human metastatic NB tissues compared with primary NB tissues. Inhibition of miR-23a may significantly suppress NB cell migration and invasion.In vitroreporter assay suggested that CDH1 is a direct target gene of miR-23a. Furthermore, blocking the expression of miR-23a partly restored the expression of CDH1 in NB cells. These findings provide evidence that miR-23a is key in promoting NB cell migration and invasion through targeting CDH1, and suggest that exogenous miR-23a may have therapeutic value in treating NB metastasis.
authors: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China.
authors: Lin
authors: L
authors: Cheng
authors: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China.
authors: Tao
authors: T
authors: Yang
authors: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China.
authors: Yongqin
authors: Y
authors: Kuang
authors: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China.
authors: Bin
authors: B
authors: Kong
authors: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China.
authors: Sixun
authors: S
authors: Yu
authors: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China.
authors: Haifeng
authors: H
authors: Shu
authors: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China.
authors: Hutian
authors: H
authors: Zhou
authors: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China.
authors: Jianwen
authors: J
authors: Gu
keywords: CDH1; miR-23a; miRNA; neuroblastoma