title: X-ray crystal structure of the passenger domain of plasmid encoded toxin(Pet), an autotransporter enterotoxin from enteroaggregative Escherichia coli (EAEC).
abstract: Autotransporters (ATs) represent a superfamily of proteins produced by a variety of pathogenic bacteria, which include the pathogenic groups of Escherichia coli (E. coli) associated with gastrointestinal and urinary tract infections. We present the first X-ray structure of the passenger domain from the Plasmid-encoded toxin (Pet) a 100 kDa protein at 2.3 Å resolution which is a cause of acute diarrhea in both developing and industrialized countries. Pet is a cytoskeleton-altering toxin that induces loss of actin stress fibers. While Pet (pdb code: 4OM9) shows only a sequence identity of 50% compared to the closest related protein sequence, extracellular serine protease plasmid (EspP) the structural features of both proteins are conserved. A closer structural look reveals that Pet contains a β-pleaded sheet at the sequence region of residues 181-190, the corresponding structural domain in EspP consists of a coiled loop. Secondary, the Pet passenger domain features a more pronounced beta sheet between residues 135 and 143 compared to the structure of EspP.
authors: Departamento de Salud Pública Facultad de Medicina UNAM, Ciudad Universitaria Coyoacán 04510, D.F., Mexico; Laboratorio de Patogenicidad Bacteriana, Unidad de Hemato Oncología e Investigación, Hospital Infantil de México Federico Gómez 06720, D.F., Mexico.
authors: J
authors: J
authors: Domingo Meza-Aguilar
authors: Department of Chemistry and Biochemistry, Arizona State University, Physical Sciences BLDG D-102, Tempe, AZ 85287, USA.
authors: Petra
authors: P
authors: Fromme
authors: Instituto de Fisiología Celular UNAM, Ciudad Universitaria Coyoacán 04510, D.F., Mexico.
authors: Alfredo
authors: A
authors: Torres-Larios
authors: Instituto de Química UNAM, Ciudad Universitaria Coyoacán 04510, D.F, Mexico.
authors: Guillermo
authors: G
authors: Mendoza-Hernández
authors: Departamento de Salud Pública Facultad de Medicina UNAM, Ciudad Universitaria Coyoacán 04510, D.F., Mexico; Laboratorio de Patogenicidad Bacteriana, Unidad de Hemato Oncología e Investigación, Hospital Infantil de México Federico Gómez 06720, D.F., Mexico.
authors: Ulises
authors: U
authors: Hernandez-Chiñas
authors: Instituto de Química UNAM, Ciudad Universitaria Coyoacán 04510, D.F, Mexico.
authors: Roberto A
authors: RA
authors: Arreguin-Espinosa de Los Monteros
authors: Departamento de Salud Pública Facultad de Medicina UNAM, Ciudad Universitaria Coyoacán 04510, D.F., Mexico; Laboratorio de Patogenicidad Bacteriana, Unidad de Hemato Oncología e Investigación, Hospital Infantil de México Federico Gómez 06720, D.F., Mexico.
authors: Carlos A
authors: CA
authors: Eslava Campos
authors: Department of Chemistry and Biochemistry, Arizona State University, Physical Sciences BLDG D-102, Tempe, AZ 85287, USA. Electronic address: Raimund.Fromme@asu.edu.
authors: Raimund
authors: R
authors: Fromme
mesh_terms: D001427:Bacterial Toxins / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004768:Enterotoxins / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D008958:Models, Molecular; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012697:Serine Endopeptidases / Q000737:chemistry*
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C107189:EspP protein, E coli; C530786:Pet protein, E coli; D012697:Serine Endopeptidases
keywords: 4OM9; Autotransporter; EAEC; Passenger domain; Plasmid encoded toxin(Pet); SPATE