title: Silencing of Glucose Transporter Protein-1 by RNA Interference Inhibits Human Osteosarcoma Mg63 Cells Growth in vivo.
abstract: While knock-down of glucose transporter protein 1 (GLUT-1) inhibited various human cancer cell growth in vitro and in vivo, including osteosarcoma cell growth in vitro, there has been no report on whether knock-down of GLUT-1 by siRNA may inhibit osteosarcoma cell growth in vivo. We hypothesized that siRNA may inhibit osteosarcoma cell growth in vivo. We introduced siRNA-GLUT-1 by lentivirus into MG63 osteosarcoma cells which were xenograted into nude mice. Immunohistochemical staining, Western blot and reverse transcriptase quantitative (RT-qPCR) were used to determine GLUT-1 protein and mRNA expression of the tumor cells. The results showed the tumor volume of GLUT-1-siRNA-MG63 cells xenorafted nude mice was significantly less than that of siGFP-MG63 or MG63 cells xenografted nude mice (P < 0.05), suggesting that silencing of GLUT-1 inhibited tumor formation and growth of osteosarcoma cells in vivo. Our findings suggest that the lentiviral-mediated siRNA interference against GLUT-1 may be a valuable tool for gene therapy for osteosarcoma.
authors: Department of Orthopedics, Tong Ji Hospital, Tong Ji University, Shanghai, China.
authors: Fan
authors: F
authors: Jian
authors: Department of Orthopedics, Tong Ji Hospital, Tong Ji University, Shanghai, China.
authors: Feng
authors: F
authors: Yuan
authors: Department of Orthopedics, Tong Ji Hospital, Tong Ji University, Shanghai, China.
authors: Mei
authors: M
authors: Jiong
authors: Department of Orthopedics, Tong Ji Hospital, Tong Ji University, Shanghai, China.
authors: Xiao-zhong
authors: XZ
authors: Zhu
authors: Department of Orthopedics, Tong Ji Hospital, Tong Ji University, Shanghai, China qidongfanjian@sina.com.
authors: Guang-rong
authors: GR
authors: Yu
authors: Biochemical Laboratory of Tong Ji University, Shanghai, China.
authors: Dong-dong
authors: DD
authors: Lu
mesh_terms: D000818:Animals; D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015316:Genetic Therapy; D051272:Glucose Transporter Type 1 / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D047368:Tumor Burden
chemical_list: D051272:Glucose Transporter Type 1; D034741:RNA, Small Interfering
keywords: Gene silencing; Glucose transporter protein1 (GLUT-1); Osteosarcoma; RNA interference