title: Kappa casein gene polymorphism in local Tunisian goats.
abstract: The genetic polymorphism of the goat Kappa casein was investigated in Tunisian goats. Blood samples were collected from local goat breeds. Samples of genomic DNA were obtained from leukocytes of 175 dairy goats and regions of interest in the gene were amplified by Polymerase Chain Reaction (PCR) and then evaluated in agarose gel. For a better characterization of the single nucleotide polymorphism, a PCR-Restriction Fragment Length Polymorphism was performed employing the endonuclease DNA amplification using 459 bp primers. The PCR products of primers (459 bp) digested by restriction enzyme Alw44I produced two fragments of 459 and 381 bp. The Kappa casein allelic variants in tested animals revealed different genotypes, two of them were homozygous: AA or BB, AC or BC and CC. Genotypic frequencies were 12.5, 60.5 and 27% for AA or BB, CC and AC or BC, respectively. Identification of different variants of the Kappa casein can be used for the improvement and conservation of Tunisian local goats.
authors: Department of Animal Production, School of Higher Education in Agriculture of Mateur, Tunisia.
authors: B
authors: B
authors: Jemmali
authors: Department of Animal Production, School of Higher Education in Agriculture of Mateur, Tunisia.
authors: A
authors: A
authors: Ben Gara
authors: Regional Field crops Research Center Beja, Tunisia.
authors: H
authors: H
authors: Selmi
authors: Department of Animal Production, School of Higher Education in Agriculture of Mateur, Tunisia.
authors: Z
authors: Z
authors: Ammari
authors: Department of Animal Production, School of Higher Education in Agriculture of Mateur, Tunisia.
authors: C
authors: C
authors: Bouheni
authors: National Agricultural Institute of Tunisia, Tunisia.
authors: M
authors: M
authors: Ben Larbi
authors: Department of Animal Production, School of Higher Education in Agriculture of Mateur, Tunisia.
authors: M
authors: M
authors: Hammami
authors: Department of Animal Production, School of Higher Education in Agriculture of Mateur, Tunisia.
authors: M
authors: M
authors: Amraoui
authors: Department of Animal Production, School of Higher Education in Agriculture of Mateur, Tunisia.
authors: M
authors: M
authors: Kamoun
authors: Department of Animal Production, School of Higher Education in Agriculture of Mateur, Tunisia.
authors: H
authors: H
authors: Rouissi
authors: Department of Animal Production, School of Higher Education in Agriculture of Mateur, Tunisia.
authors: B
authors: B
authors: Rekik
mesh_terms: D000818:Animals; D002364:Caseins / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005787:Gene Frequency; D005838:Genotype; D006041:Goats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D014416:Tunisia
chemical_list: D002364:Caseins