title: Overview of affinity tags for protein purification.
abstract: Addition of an affinity tag is a useful method for differentiating recombinant proteins expressed in bacterial and eukaryotic expression systems from the background of total cellular proteins, as well as for detecting protein-protein interactions. This overview describes the historical basis for the development of affinity tags, affinity tags that are commonly used today, how to choose an appropriate affinity tag for a particular purpose, and several recently developed affinity tag technologies that may prove useful in the near future.
authors: University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin.
authors: Michelle E
authors: ME
authors: Kimple
authors: University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin.
authors: Allison L
authors: AL
authors: Brill
authors: University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin.
authors: Renee L
authors: RL
authors: Pasker
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D015336:Molecular Probe Techniques; D011994:Recombinant Proteins / Q000302:isolation & purification*
chemical_list: D011994:Recombinant Proteins
keywords: affinity chromatography; affinity tag; epitope tag; protein detection; protein purification