title: Transplantation of bone marrow mesenchymal stem cells decreases oxidative stress, apoptosis, and hippocampal damage in brain of a spontaneous stroke model.
abstract: Stroke is the most common cause of motor disabilities and is a major cause of mortality worldwide. Adult stem cells have been shown to be effective against neuronal degeneration through mechanisms that include both the recovery of neurotransmitter activity and a decrease in apoptosis and oxidative stress. We chose the lineage stroke-prone spontaneously hypertensive rat (SHRSP) as a model for stem cell therapy. SHRSP rats can develop such severe hypertension that they generally suffer a stroke at approximately 1 year of age. The aim of this study was to evaluate whether mesenchymal stem cells (MSCs) decrease apoptotic death and oxidative stress in existing SHRSP brain tissue. The results of qRT-PCR assays showed higher levels of the antiapoptotic Bcl-2 gene in the MSC-treated animals, compared with untreated. Our study also showed that superoxide, apoptotic cells, and by-products of lipid peroxidation decreased in MSC-treated SHRSP to levels similar those found in the animal controls, Wistar Kyoto rats. In addition, we saw a repair of morphological damage at the hippocampal region after MSC transplantation. These data suggest that MSCs have neuroprotective and antioxidant potential in stroke-prone spontaneously hypertensive rats.
authors: Departamento de Biofísica, Universidade Federal de São Paulo, São Paulo, SP 04023-062, Brazil.
authors: Michele Longoni
authors: ML
authors: Calió
authors: Centro de Desenvolvimento de Modelos Experimentais para Medicina e Biologia, Universidade Federal de São Paulo, São Paulo, SP 04023-062, Brazil.
authors: Darci Sousa
authors: DS
authors: Marinho
authors: Centro de Desenvolvimento de Modelos Experimentais para Medicina e Biologia, Universidade Federal de São Paulo, São Paulo, SP 04023-062, Brazil.
authors: Gui Mi
authors: GM
authors: Ko
authors: Departamento de Biofísica, Universidade Federal de São Paulo, São Paulo, SP 04023-062, Brazil.
authors: Renata Rodrigues
authors: RR
authors: Ribeiro
authors: Departamento de Morfologia, Universidade Federal de São Paulo, São Paulo, SP 04023-062, Brazil.
authors: Adriana Ferraz
authors: AF
authors: Carbonel
authors: Departamento de Fisiologia, Universidade Federal de São Paulo, São Paulo, SP 04023-062, Brazil.
authors: Lila Missae
authors: LM
authors: Oyama
authors: Centro de Desenvolvimento de Modelos Experimentais para Medicina e Biologia, Universidade Federal de São Paulo, São Paulo, SP 04023-062, Brazil.
authors: Milene
authors: M
authors: Ormanji
authors: Centro de Desenvolvimento de Modelos Experimentais para Medicina e Biologia, Universidade Federal de São Paulo, São Paulo, SP 04023-062, Brazil.
authors: Tatiana Pinoti
authors: TP
authors: Guirao
authors: Departamento de Odontologia, Universidade Santa Cecília, Santos, SP, Brazil.
authors: Pedro Luiz
authors: PL
authors: Calió
authors: Departamento de Nefrologia, Universidade Federal de São Paulo, São Paulo, SP 04023-062, Brazil.
authors: Luciana Aparecida
authors: LA
authors: Reis
authors: Departamento de Morfologia, Universidade Federal de São Paulo, São Paulo, SP 04023-062, Brazil.
authors: Manuel de Jesus
authors: Mde J
authors: Simões
authors: Departamento de Biofísica, Universidade Federal de São Paulo, São Paulo, SP 04023-062, Brazil.
authors: Telma
authors: T
authors: Lisbôa-Nascimento
authors: Departamento de Biofísica, Universidade Federal de São Paulo, São Paulo, SP 04023-062, Brazil.
authors: Alice Teixeira
authors: AT
authors: Ferreira
authors: Centro de Desenvolvimento de Modelos Experimentais para Medicina e Biologia, Universidade Federal de São Paulo, São Paulo, SP 04023-062, Brazil. Electronic address: clelia.rejane@unifesp.br.
authors: Clélia Rejane Antônio
authors: CR
authors: Bertoncini
mesh_terms: D000818:Animals; D017209:Apoptosis; D016026:Bone Marrow Transplantation; D005609:Free Radicals / Q000378:metabolism; D006624:Hippocampus / Q000293:injuries / Q000601:surgery; D006801:Humans; D015227:Lipid Peroxidation / Q000235:genetics*; D045164:Mesenchymal Stem Cell Transplantation; D018384:Oxidative Stress; D051381:Rats; D011918:Rats, Inbred SHR; D017382:Reactive Oxygen Species / Q000378:metabolism; D020521:Stroke / Q000378:metabolism / Q000473:pathology / Q000628:therapy*
chemical_list: D005609:Free Radicals; D017382:Reactive Oxygen Species
keywords: Apoptosis; Free radicals; Lipid peroxidation; Mesenchymal stem cells; Stroke; Superoxide