title: Nonenzymatic immunosensor for detection of carbohydrate antigen 15-3 based on hierarchical nanoporous PtFe alloy.
abstract: Bimetallic hierarchical nanostructures have attracted increasing attention for biosensing applications due to their higher electrocatalytic activity and better biocompatibility. In this work, we developed a novel sandwich-type nonenzymatic electrochemical immunosensor for cancer biomarker carbohydrate antigen 15-3 (CA15-3). Hierarchical nanoporous PtFe alloys were used as carriers of the signaling antibody anti-CA15-3 in order to achieve an signal amplification. The immunosensor is constructed by using a graphene as platform and using mesoporous PtFe alloy as labels. The electrochemical signal of the immunosensor was based on the high-electrocatalytic activity of PtFe alloy for the electro-oxidation of H2O2. Under optimal conditions, this method could detect CA15-3 ranging from 0.002 to 40 U/mL with a low detection limit of 3 × 10(-4)U/mL. This immunosensor showed good accuracy, stability and fabrication reproducibility. The hierarchical nanoporous metal materials-based immunoassay provides a promising ultrasensitive immunoassay approach for clinical applications.
authors: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors: Yuyang
authors: Y
authors: Li
authors: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors: Caixia
authors: C
authors: Xu
authors: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors: He
authors: H
authors: Li
authors: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors: Huan
authors: H
authors: Wang
authors: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors: Dan
authors: D
authors: Wu
authors: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors: Hongmin
authors: H
authors: Ma
authors: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors: Yanyan
authors: Y
authors: Cai
authors: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors: Bin
authors: B
authors: Du
authors: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China. Electronic address: sdjndxwq@163.com.
authors: Qin
authors: Q
authors: Wei
mesh_terms: D000497:Alloys / Q000737:chemistry*; D055910:Antibodies, Immobilized / Q000737:chemistry; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis / Q000097:blood*; D015374:Biosensing Techniques / Q000379:methods; D055664:Electrochemical Techniques / Q000379:methods; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D007118:Immunoassay / Q000379:methods*; D007501:Iron / Q000737:chemistry*; D057230:Limit of Detection; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010984:Platinum / Q000737:chemistry*; D016062:Porosity; D015203:Reproducibility of Results
chemical_list: D000497:Alloys; D055910:Antibodies, Immobilized; D015295:Antigens, Tumor-Associated, Carbohydrate; D010984:Platinum; D006861:Hydrogen Peroxide; D007501:Iron
keywords: Carbohydrate antigen 15-3; Hierarchical nanoporous; Nonenzymatic electrochemical immunosensor; PtFe alloy