title: Bioelectrocatalytic sensor for triglycerides in human skin sebum based on enzymatic cascade reaction of lipase, glycerol kinase and glycerophosphate oxidase.
abstract: We report the development of an electrochemical biosensor for the quantification of triglycerides in human skin sebum, based on a multienzyme cascade reaction. The presence of excessive triglycerides in human sebum is one of the leading causes of various skin ailments. However, to the best of our knowledge, no bioelectrocatalytic approach for the quantification of sebum triglycerides has been made. In order to develop triglyceride biosensor, we fabricated a multienzyme-associated electrode incorporating lipase, glycerol kinase, and glycerophosphate oxidase. Enzymes were deposited by electrostatic force and further stabilized via crosslinking between enzymes and polymer matrices. The enzyme-modified biosensing electrode maintained its bioelectrocatalytic activity for five days. An additional constraint was the limited solubility of sebum triglycerides in aqueous electrolytes, impeding the analysis. To address this issue, triglyceride samples were prepared in the form of micelles, enabling efficient sample preparation for biosensor signaling. Calibration tests revealed that the designed assay had a detection range of 15-200mg/dL of micellar triglyceride, which covered the required determination range. The developed biosensing approach was successfully used to determine triglyceride concentrations in real sebum samples of unknown triglyceride content.
authors: Department of Molecular Science and Technology, Ajou University, Yeongtong-gu, Suwon 443-749, South Korea.
authors: Chi Yong
authors: CY
authors: Jeong
authors: Department of Molecular Science and Technology, Ajou University, Yeongtong-gu, Suwon 443-749, South Korea.
authors: Yong Duk
authors: YD
authors: Han
authors: Department of Molecular Science and Technology, Ajou University, Yeongtong-gu, Suwon 443-749, South Korea.
authors: Jae Ho
authors: JH
authors: Yoon
authors: Department of Molecular Science and Technology, Ajou University, Yeongtong-gu, Suwon 443-749, South Korea. Electronic address: hcyoon@ajou.ac.kr.
authors: Hyun C
authors: HC
authors: Yoon
mesh_terms: D055162:Biocatalysis; D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D004798:Enzymes / Q000378:metabolism; D005991:Glycerol Kinase / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D006801:Humans; D008049:Lipase / Q000378:metabolism; D008823:Micelles; D012629:Sebum / Q000737:chemistry* / Q000378:metabolism; D014280:Triglycerides / Q000032:analysis*
chemical_list: D004798:Enzymes; D008823:Micelles; D014280:Triglycerides; D005993:Glycerolphosphate Dehydrogenase; D005991:Glycerol Kinase; D008049:Lipase
keywords: Bioelectrocatalytic; Enzyme cascade; Skin sebum triglyceride; Triglyceride biosensor