title: Proteomic analysis indicates that overexpression and nuclear translocation of lactoylglutathione lyase (GLO1) is associated with tumor progression in murine fibrosarcoma.
abstract: Lactoylglutathione lyase (GLO1), a ubiquitously expressed methylglyoxal (MG) detoxification enzyme, is implicated in the progression of various human malignant diseases. However, the role of GLO1 in the development or progression of murine fibrosarcoma is still unclear. We performed proteomic analysis to identify differences in the intracellular proteins of the regressive tumor cell line QR-32 and the inflammatory cell-promoting progressive tumor cell line QRsP-11 of murine fibrosarcoma by 2DE combined with MS. Seven upregulated proteins were identified in QRsP-11 compared to QR-32 cells, namely, GLO1, annexin A1, adenylate kinase isoenzyme 1, transcription factor BTF3, myosin light polypeptide 6, low molecular weight phosphotyrosine protein phosphatase and nucleoside diphosphate kinase B. Heat shock protein beta-1 (HspB1), a methylglyoxal-adducted protein, is concomitantly over-expressed in QRsP-11 as compared to QR-32 cells. We also found out that GLO1 is translocated into the nucleus to a higher extent in QRsP-11 compared to QR-32 cells, which can be reversed by using a MEK inhibitor (U0126). Moreover, U0126 and GLO1 siRNA can inhibit cell proliferation and migration in QRsP-11 cells. Our data suggest that overexpression and nuclear translocation of GLO1 might be associated with tumor progression in murine fibrosarcoma.
authors: Departments of Biochemistry and Functional Proteomics Yamaguchi University Graduate School of Medicine, Ube, Japan.
authors: Yufeng
authors: Y
authors: Wang
authors: Yasuhiro
authors: Y
authors: Kuramitsu
authors: Kazuhiro
authors: K
authors: Tokuda
authors: Futoshi
authors: F
authors: Okada
authors: Byron
authors: B
authors: Baron
authors: Junko
authors: J
authors: Akada
authors: Takao
authors: T
authors: Kitagawa
authors: Kazuyuki
authors: K
authors: Nakamura
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism*; D049109:Cell Proliferation; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D005354:Fibrosarcoma / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000378:metabolism; D007791:Lactoylglutathione Lyase / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D051379:Mice; D018832:Molecular Chaperones; D009363:Neoplasm Proteins / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D006360:Heat-Shock Proteins; C486113:Hsbp1 protein, mouse; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; D020543:Proteome; D034741:RNA, Small Interfering; D007791:Lactoylglutathione Lyase
keywords: 2DE; GLO1; LC-MS/MS; Murine fibrosarcoma