title: Association of immune and metabolic receptors C5aR and C5L2 with adiposity in women.
abstract: Adipose tissue receptors C5aR and C5L2 and their heterodimerization/functionality and interaction with ligands C5a and acylation stimulating protein (ASP) have been evaluated in cell and rodent studies. Their contribution to obesity factors in humans remains unclear. We hypothesized that C5a receptors, classically required for host defense, are also associated with adiposity. Anthropometry and fasting blood parameters were measured in 136 women divided by body mass index (BMI): normal/overweight (≤30 kg/m(2); n = 34), obese I (≤45 kg/m(2); n = 33), obese II (≤51 kg/m(2); n = 33), and obese III (≤80 kg/m(2); n = 36). Subcutaneous and omental adipose tissue C5aR and C5L2 expression were analysed. C5L2 expression was comparable between subcutaneous and omental across all BMI groups. Plasma ASP and ASP/omental C5L2 expression increased with BMI (P < 0.001 and P < 0.01, resp.). While plasma C5a was unchanged, C5aR expression decreased with increasing BMI in subcutaneous and omental tissues (P < 0.01 and P < 0.05, resp.), with subcutaneous omental depots. Omental C5L2/C5aR ratio increased with BMI (P < 0.01) with correlations between C5L2/C5aR and waist circumference, HDL-C, and adiponectin. Tissue and BMI differences in receptors and ligands, particularly in omental, suggest relationship to metabolic disturbances and highlight adipose-immune interactions.
authors: Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Québec (CRIUCPQ), Laval University, Y4323, 2725 Chemin Ste-Foy, Québec, QC, Canada G1V 4G5 ; Department of Medicine, Laval University, 1050 Avenue de la Médecine, Québec, QC, Canada G1V 0A6.
authors: Pegah
authors: 0000-0002-7804-4359
authors: P
authors: Poursharifi
authors: Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Québec (CRIUCPQ), Laval University, Y4323, 2725 Chemin Ste-Foy, Québec, QC, Canada G1V 4G5.
authors: Reza
authors: R
authors: Rezvani
authors: Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Québec (CRIUCPQ), Laval University, Y4323, 2725 Chemin Ste-Foy, Québec, QC, Canada G1V 4G5.
authors: Abhishek
authors: A
authors: Gupta
authors: Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Québec (CRIUCPQ), Laval University, Y4323, 2725 Chemin Ste-Foy, Québec, QC, Canada G1V 4G5.
authors: Marc
authors: 0000-0003-0461-2576
authors: M
authors: Lapointe
authors: Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Québec (CRIUCPQ), Laval University, Y4323, 2725 Chemin Ste-Foy, Québec, QC, Canada G1V 4G5.
authors: Picard
authors: P
authors: Marceau
authors: Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Québec (CRIUCPQ), Laval University, Y4323, 2725 Chemin Ste-Foy, Québec, QC, Canada G1V 4G5.
authors: André
authors: A
authors: Tchernof
authors: Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Québec (CRIUCPQ), Laval University, Y4323, 2725 Chemin Ste-Foy, Québec, QC, Canada G1V 4G5 ; Department of Medicine, Laval University, 1050 Avenue de la Médecine, Québec, QC, Canada G1V 0A6.
authors: Katherine
authors: 0000-0002-1760-2679
authors: K
authors: Cianflone
mesh_terms: D052242:Adiponectin / Q000097:blood; D000273:Adipose Tissue / Q000378:metabolism; D050154:Adiposity; D000328:Adult; D000368:Aged; D000886:Anthropometry; D015992:Body Mass Index; D008076:Cholesterol, HDL / Q000097:blood; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007107:Immune System; D008875:Middle Aged; D009765:Obesity; D009852:Omentum / Q000378:metabolism; D044087:Receptor, Anaphylatoxin C5a / Q000378:metabolism*; D019707:Receptors, Chemokine / Q000378:metabolism*; D055815:Young Adult
chemical_list: D052242:Adiponectin; C488033:C5AR1 protein, human; C417003:C5aR2 protein, human; D008076:Cholesterol, HDL; D044087:Receptor, Anaphylatoxin C5a; D019707:Receptors, Chemokine