title: Profile of natural killer cells after a previous natural Vaccinia virus infection in an in vitro viral re-exposure.
abstract: The present study compares the profile of NK cells in an in vitro re-exposure by Vaccinia virus (VACV), in groups that have had a previous vaccination or natural infection. Our data suggests that stimulation with VACV triggers a cytotoxic response by NK cells marked by an increase of NCRs: NKp30, NKp44, and NKp46 in infected (vaccinated and unvaccinated) subjects and in non-infected vaccinated patients, when compared with non-infected unvaccinated individuals. However, the degranulation and secretion processes are inhibited in infected (vaccinated and unvaccinated) subjects and in the non-infected vaccinated patients, when compared with non-infected unvaccinated individuals. We demonstrated that stimulation with VACV downregulates the percentage of expression of Perforin, Granzyme A, and CD107a, but upregulate CD94 in infected (vaccinated and unvaccinated) subjects and in non-infected vaccinated patients, when compared with non-infected unvaccinated individuals. Furthermore, the percentage of IFN-γ(+) NK cells was significantly lower in non-infected unvaccinated subjects, when compared with infected (vaccinated and unvaccinated) and non-infected vaccinated individuals. Our results also show that the percentage of TNF-α(+) NK cells was significantly higher in infected (vaccinated and unvaccinated) subjects and in non-infected vaccinated patients, when compared with non-infected unvaccinated individuals, after in vitro stimulation with UV-inactivated VACV. Our data suggest that the expression of NCRs NKp30, NKp44, NKp46 and cytokines by NK cells are important in the innate response against VACV.
authors: Centro de Pesquisas René Rachou (CPqRR), Fundação Oswaldo Cruz (Fiocruz), Ministério da Saúde, Belo Horizonte, MG, Brazil; Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil. Electronic address: duduaugusto1@yahoo.com.br.
authors: Eduardo Augusto dos Santos
authors: EA
authors: Moreira-Silva
authors: Centro de Pesquisas René Rachou (CPqRR), Fundação Oswaldo Cruz (Fiocruz), Ministério da Saúde, Belo Horizonte, MG, Brazil; Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
authors: Daniela Carla
authors: DC
authors: Medeiros-Silva
authors: Departamento de Morfologia, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
authors: Juliana de Assis Silva
authors: Jde A
authors: Gomes
authors: Departamento de Microbiologia, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
authors: Flávio Guimarães
authors: FG
authors: da Fonseca
authors: Centro de Pesquisas René Rachou (CPqRR), Fundação Oswaldo Cruz (Fiocruz), Ministério da Saúde, Belo Horizonte, MG, Brazil; Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
authors: Rodrigo
authors: R
authors: Correa-Oliveira
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D015550:Cell Degranulation; D016207:Cytokines / Q000378:metabolism; D003602:Cytotoxicity, Immunologic; D005260:Female; D053804:Granzymes / Q000032:analysis; D006801:Humans; D007694:Killer Cells, Natural / Q000737:chemistry / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D055627:Natural Cytotoxicity Triggering Receptor 1 / Q000032:analysis; D055629:Natural Cytotoxicity Triggering Receptor 2 / Q000032:analysis; D055628:Natural Cytotoxicity Triggering Receptor 3 / Q000032:analysis; D054353:Perforin / Q000032:analysis; D012900:Smallpox Vaccine / Q000008:administration & dosage / Q000276:immunology*; D014615:Vaccinia / Q000276:immunology*; D014616:Vaccinia virus / Q000276:immunology*; D055815:Young Adult
chemical_list: D015703:Antigens, CD; D016207:Cytokines; C112260:NCR1 protein, human; C112837:NCR2 protein, human; C431710:NCR3 protein, human; D055627:Natural Cytotoxicity Triggering Receptor 1; D055629:Natural Cytotoxicity Triggering Receptor 2; D055628:Natural Cytotoxicity Triggering Receptor 3; D012900:Smallpox Vaccine; D054353:Perforin; D053804:Granzymes
keywords: NK cells; Poxviridae infections; Smallpox vaccine; Vaccinia virus; Zoonoses