title: Production of (+)-valencene in the mushroom-forming fungus S. commune.
abstract: Production of commercially interesting sesquiterpenes was previously examined in plants and microorganisms such as Escherichia coli and Saccharomyces cerevisiae. We here investigate the potential of the mushroom Schizophyllum commune for the production of sesquiterpenes. Genomic analysis of S. commune revealed that the mevalonate pathway required for the synthesis of the farnesyl diphosphate substrate for sesquiterpene production is operational. Introduction of a valencene synthase gene resulted in production of the sesquiterpene (+)-valencene, both in mycelium and in fruiting bodies. Levels of (+)-valencene in culture media of strains containing a mutated RGS regulatory protein gene (thn) were increased fourfold compared to those in wild-type transformants. Up to 16 mg L(-1) (+)-valencene was produced in these strains. In addition, the amount of (+)-valencene containing n-dodecane recovered from the culture medium increased sixfold to sevenfold in the thn mutant strains due to the absence of schizophyllan.
authors: Department of Microbiology, Kluyver Centre for Genomics of Industrial Fermentation, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands, k.scholtmeijer@uu.nl.
authors: Karin
authors: K
authors: Scholtmeijer
authors: Katarina
authors: K
authors: Cankar
authors: Jules
authors: J
authors: Beekwilder
authors: Han A B
authors: HA
authors: Wösten
authors: Luis G
authors: LG
authors: Lugones
authors: Dirk
authors: D
authors: Bosch
mesh_terms: D000473:Alkanes / Q000032:analysis; D003470:Culture Media / Q000737:chemistry; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D060847:Metabolic Engineering; D053858:Metabolic Networks and Pathways / Q000235:genetics; D008969:Molecular Sequence Data; D012567:Schizophyllum / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D012717:Sesquiterpenes / Q000378:metabolism*
chemical_list: D000473:Alkanes; D003470:Culture Media; D004271:DNA, Fungal; D012717:Sesquiterpenes; C007548:n-dodecane; C506706:valencene