title: Participation of bone marrow-derived cells in hippocampal vascularization after status epilepticus.
abstract: OBJECTIVE
  Diseases such as temporal lobe epilepsy, brain trauma and stroke can induce endothelial cell proliferation and angiogenesis in specific brain areas. During status epilepticus (SE), bone marrow-derived cells are able to infiltrate and proliferate, dramatically increasing at the site of injury. However, it is still unclear whether these cells directly participate in vascular changes induced by SE.
  
  
  METHODS
  To investigate the possible role of bone marrow-derived cells in angiogenesis after seizures, we induced SE by pilocarpine injection in previously prepared chimeric mice. Mice were euthanized at 8h, 7d or 15d after SE onset.
  
  
  RESULTS
  Our results indicated that SE modified hippocampal vascularization and induced angiogenesis. Further, bone marrow-derived GFP(+) cells penetrated through the parenchyma and participated in the formation of new vessels after SE. We detected bone marrow-derived cells closely associated with vessels in the hippocampus, increasing the density of blood vessels that had decreased immediately after pilocarpine-induced SE.
  
  
  CONCLUSIONS
  We conclude that epileptic seizures directly affect vascularization in the hippocampus mediated by bone marrow-derived cells in a time-dependent manner.
authors: Department of Physiology, Federal University of São Paulo, Rua Botucatu, 862, 04023-062 São Paulo, SP, Brazil.
authors: Simone A
authors: SA
authors: Romariz
authors: Department of Physiology, Federal University of São Paulo, Rua Botucatu, 862, 04023-062 São Paulo, SP, Brazil.
authors: Karina de O
authors: Kde O
authors: Garcia
authors: Department of Physiology, Federal University of São Paulo, Rua Botucatu, 862, 04023-062 São Paulo, SP, Brazil.
authors: Daisyléa de Souza
authors: Dde S
authors: Paiva
authors: Department of Physiology, Federal University of São Paulo, Rua Botucatu, 862, 04023-062 São Paulo, SP, Brazil.
authors: Simone
authors: S
authors: Bittencourt
authors: Department of Physiology, Federal University of São Paulo, Rua Botucatu, 862, 04023-062 São Paulo, SP, Brazil.
authors: Luciene
authors: L
authors: Covolan
authors: Department of Physiology, Federal University of São Paulo, Rua Botucatu, 862, 04023-062 São Paulo, SP, Brazil.
authors: Luis Eugênio
authors: LE
authors: Mello
authors: Department of Physiology, Federal University of São Paulo, Rua Botucatu, 862, 04023-062 São Paulo, SP, Brazil. Electronic address: beatriz.longo@unifesp.br.
authors: Beatriz Monteiro
authors: BM
authors: Longo
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D004833:Epilepsy, Temporal Lobe / Q000139:chemically induced / Q000473:pathology; D006624:Hippocampus / Q000098:blood supply / Q000166:cytology* / Q000187:drug effects*; D008810:Mice, Inbred C57BL; D010862:Pilocarpine / Q000633:toxicity*; D012640:Seizures / Q000139:chemically induced / Q000473:pathology; D013226:Status Epilepticus / Q000139:chemically induced / Q000473:pathology*; D013997:Time Factors
chemical_list: D010862:Pilocarpine
keywords: Acute seizures; Blood vessels; Chimeric mice; GFP; Hippocampus; Pilocarpine