title: Choose your label wisely: water-soluble fluorophores often interact with lipid bilayers.
abstract: Water-soluble organic fluorophores are widely used as labels in biological systems. However, in many cases these fluorophores can interact strongly with lipid bilayers, influencing the interaction of the target with the bilayer and/or leading to misleading fluorescent signals. Here, we quantify the interaction of 32 common water-soluble dyes with model lipid bilayers to serve as an additional criterion when selecting a dye label.
authors: Department of Chemistry, Stanford University, Stanford, California, United States.
authors: Laura D
authors: LD
authors: Hughes
authors: Department of Chemistry, Stanford University, Stanford, California, United States.
authors: Robert J
authors: RJ
authors: Rawle
authors: Department of Chemistry, Stanford University, Stanford, California, United States.
authors: Steven G
authors: SG
authors: Boxer
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D012995:Solubility; D013050:Spectrometry, Fluorescence; D014867:Water
chemical_list: D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D011506:Proteins; D014867:Water