title: Selection and screening using antibody phage display libraries.
abstract: Phage display is a powerful tool to isolate specific binders from a large and diverse combinatorial library. Here we provide a step-by-step protocol in how to set up a successful phage panning experiment in order to isolate useful antibodies. The protocol includes testing antigens for their suitability in the phage panning procedure and optimizing the panning conditions and alternative screening methods to minimize nonspecific binding. We describe example phage panning experiments starting from the library transformation to the phage clone screening.
authors: Antibody Engineering Department, Genentech Inc., South San Francisco, CA, USA.
authors: Patrick
authors: P
authors: Koenig
authors: Germaine
authors: G
authors: Fuh
mesh_terms: D000906:Antibodies / Q000378:metabolism*; D019151:Peptide Library
chemical_list: D000906:Antibodies; D019151:Peptide Library