title: Trigger factor assists the refolding of heterodimeric but not monomeric luciferases.
abstract: The refolding of thermally inactivated protein by ATP-independent trigger factor (TF) and ATP-dependent DnaKJE chaperones was comparatively analyzed. Heterodimeric (αβ) bacterial luciferases of Aliivibrio fischeri, Photobacterium leiognathi, and Vibrio harveyi as well as monomeric luciferases of Vibrio harveyi and Luciola mingrelica (firefly) were used as substrates. In the presence of TF, thermally inactivated heterodimeric bacterial luciferases refold, while monomeric luciferases do not refold. These observations were made both in vivo (Escherichia coli ΔdnaKJ containing plasmids with tig gene) and in vitro (purified TF). Unlike TF, the DnaKJE chaperone system refolds both monomeric and heterodimeric luciferases with equal efficiency.
authors: Research Institute for Genetics and Selection of Industrial Microorganisms (GosNIIGenetika), Moscow, 117545, Russia. zavilgel@genetika.ru.
authors: O E
authors: OE
authors: Melkina
authors: I I
authors: II
authors: Goryanin
authors: I V
authors: IV
authors: Manukhov
authors: A V
authors: AV
authors: Baranova
authors: V A
authors: VA
authors: Kolb
authors: M S
authors: MS
authors: Svetlov
authors: G B
authors: GB
authors: Zavilgelsky
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019281:Dimerization; D004926:Escherichia coli / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000378:metabolism; D048888:Fireflies / Q000201:enzymology; D049453:Luciferases, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D049409:Luciferases, Firefly / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018832:Molecular Chaperones / Q000378:metabolism; D010776:Photobacterium / Q000201:enzymology; D058849:Protein Refolding; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D014733:Vibrio / Q000201:enzymology
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D018832:Molecular Chaperones; D011994:Recombinant Proteins; D049409:Luciferases, Firefly; D049453:Luciferases, Bacterial