title: A new rotating-disk sorptive extraction mode, with a copolymer of divinylbenzene and N-vinylpyrrolidone trapped in the cavity of the disk, used for determination of florfenicol residues in porcine plasma.
abstract: A novel extraction approach was developed based on rotating-disk sorptive extraction (RDSE). In this approach the rotating-disk extraction device consists of a Teflon disk, with a cavity that is loaded with a commercial sorbent phase selected according to the polarity of the analyte. To avoid leakage of the sorbent, the cavity is covered with a fiberglass filter and sealed with a Teflon ring. The proposed novel analytical RDSE technique was used in this study to determine florfenicol levels in plasma as a model analyte, or sample system, to describe the pharmacokinetics of a veterinary formulation. The sorbent used for this application was the copolymer of divinylbenzene and N-vinylpyrrolidone (Oasis HLB), which was selected because the florfenicol molecule contains both hydrophilic and lipophilic moieties. After the extraction, final determination of the analyte was performed by HPLC-DAD. Calibration plots and other analytical features were obtained after 90 min of extraction. The calibration plot was linear over the interval 0.4-16 μg mL(-1) (n = 6), with R (2) = 0.9999. Recovery and repeatability were determined using a blank plasma sample spiked with 4.8 μg mL(-1) florfenicol. A recovery of 91.5 %, with a relative standard deviation (RSD) of 8.8 %, was obtained when the extraction was evaluated using six different rotating-disk devices. Precision was also assessed, using the same disk (containing the same sorbent phase) for eight aliquots of the same sample. The RSD under these conditions was 10.2 %, clearly indicating that the sorptive phase could possibly be re-used. Accordingly, RDSE is a suitable sample preparation alternative to liquid-liquid extraction (LLE), solid-phase extraction (SPE), and stir-bar sorptive extraction (SBSE).
authors: Department of Inorganic and Analytical Chemistry, Faculty of Chemical and Pharmaceutical Sciences, University of Chile, PO Box 653, Santiago, Chile.
authors: Alejandro
authors: A
authors: Cañas
authors: Samuel
authors: S
authors: Valdebenito
authors: Pablo
authors: P
authors: Richter
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000032:analysis / Q000302:isolation & purification*; D057230:Limit of Detection; D011145:Polyvinyls / Q000737:chemistry; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D013552:Swine; D013839:Thiamphenicol / Q000031:analogs & derivatives* / Q000097:blood / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D011145:Polyvinyls; C530109:divinylbenzene-N-vinylpyrrolidone copolymer; C035534:florfenicol; D013839:Thiamphenicol