title: Electroporation-mediated gene delivery to the lungs.
abstract: Electroporation is a safe, efficient, and inexpensive method to transfer naked plasmid DNA into various tissues. For electroporation-mediated gene transfer to the mouse lung, a plasmid solution is delivered to the lungs via the trachea. Immediately after plasmid delivery, eight square wave pulses are delivered by two pre-gelled electrodes placed on each side of the chest. An optimal field strength in mice is 200 V/cm, with a pulse duration of 10 ms each and a 1 s interval between pulses. High level gene expression can be achieved within 24 h in all cell types in the lung with very little inflammation and no apparent trauma.
authors: Division of Neonatology, Department of Pediatrics, School of Medicine and Dentistry, University of Rochester, Rochester, NY, USA.
authors: Jennifer L
authors: JL
authors: Young
authors: Michael S
authors: MS
authors: Barravecchia
authors: David A
authors: DA
authors: Dean
mesh_terms: D000758:Anesthesia; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D018274:Electroporation / Q000295:instrumentation / Q000379:methods*; D018014:Gene Transfer Techniques / Q000295:instrumentation; D008168:Lung / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D016612:Paraffin Embedding; D010477:Perfusion; D011506:Proteins / Q000302:isolation & purification; D012313:RNA / Q000235:genetics / Q000302:isolation & purification
chemical_list: D011506:Proteins; D012313:RNA