title: Assembly and analysis of a male sterile rubber tree mitochondrial genome reveals DNA rearrangement events and a novel transcript.
abstract: BACKGROUND
  The rubber tree, Hevea brasiliensis, is an important plant species that is commercially grown to produce latex rubber in many countries. The rubber tree variety BPM 24 exhibits cytoplasmic male sterility, inherited from the variety GT 1.
  
  
  RESULTS
  We constructed the rubber tree mitochondrial genome of a cytoplasmic male sterile variety, BPM 24, using 454 sequencing, including 8 kb paired-end libraries, plus Illumina paired-end sequencing. We annotated this mitochondrial genome with the aid of Illumina RNA-seq data and performed comparative analysis. We then compared the sequence of BPM 24 to the contigs of the published rubber tree, variety RRIM 600, and identified a rearrangement that is unique to BPM 24 resulting in a novel transcript containing a portion of atp9.
  
  
  CONCLUSIONS
  The novel transcript is consistent with changes that cause cytoplasmic male sterility through a slight reduction to ATP production efficiency. The exhaustive nature of the search rules out alternative causes and supports previous findings of novel transcripts causing cytoplasmic male sterility.
authors: Jeremy R
authors: JR
authors: Shearman
authors: Duangjai
authors: D
authors: Sangsrakru
authors: Panthita
authors: P
authors: Ruang-Areerate
authors: Chutima
authors: C
authors: Sonthirod
authors: Pichahpuk
authors: P
authors: Uthaipaisanwong
authors: Thippawan
authors: T
authors: Yoocha
authors: Supannee
authors: S
authors: Poopear
authors: Kanikar
authors: K
authors: Theerawattanasuk
authors: Somvong
authors: S
authors: Tragoonrung
authors: National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Paholyothin Road, Khlong Nueng, Khlong Luang, Pathumthani 12120, Thailand. sithichoke.tan@biotec.or.th.
authors: Sithichoke
authors: S
authors: Tangphatsornruang
mesh_terms: D000255:Adenosine Triphosphate; D018506:Gene Expression Regulation, Plant; D054629:Genome, Mitochondrial / Q000235:genetics*; D028482:Hevea / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D000255:Adenosine Triphosphate