title: Rapid generation of cell gradients by utilizing solely nanotopographic interactions on a bio-inert glass surface.
abstract: The control of cell gradients is critical for understanding many biological systems and realizing the unique functionality of biomimetic implants. Herein, we report a nanotopographic gradient strategy that can rapidly generate cell gradients on a nanodendritic silica substrate without any chemical modification. We can achieve controllable cell gradients within only half an hour of cell incubation solely induced by the topographic effect of the gradient nanodendrites. We also demonstrate that cell gradients can be modulated by the combination of nanotopographic and chemical gradients. The results reveal that the enhanced topographic interactions between the nanodentritic structure and nanoscaled filopodia of the cells mainly contribute to the generation of cell gradients.
authors: Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Organic Solids Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 (P.R. China); University of Chinese Academy of Sciences, Beijing 100049 (P.R. China).
authors: Gao
authors: G
authors: Yang
authors: Yanhua
authors: Y
authors: Cao
authors: Junbing
authors: J
authors: Fan
authors: Hongliang
authors: H
authors: Liu
authors: Feilong
authors: F
authors: Zhang
authors: Pengchao
authors: P
authors: Zhang
authors: Chao
authors: C
authors: Huang
authors: Lei
authors: L
authors: Jiang
authors: Shutao
authors: S
authors: Wang
mesh_terms: D000818:Animals; D050091:Dendrimers / Q000737:chemistry; D005898:Glass / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D018625:Microscopy, Atomic Force; D041681:NIH 3T3 Cells; D049329:Nanostructures / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties
chemical_list: D050091:Dendrimers; D012822:Silicon Dioxide
keywords: cell gradients; chemical modification; nanotopographic gradient; topographic interaction