title: Molecular detection of Ancylostoma duodenale, Ancylostoma ceylanicum, and Necator americanus in humans in northeastern and southern Thailand.
abstract: The 2 principal species of hookworms infecting humans are Necator americanus and Ancylostoma duodenale. Case studies on zoonotic hookworm infections with Ancylostoma ceylanicum and/or Ancylostoma caninum are known mainly from Asian countries. Of these 2 zoonotic species, only A. ceylanicum can develop to adulthood in humans. In the present study, we report a molecular-based survey of human hookworm infections present in southern and northeastern Thailand. Thirty larval hookworm samples were obtained from fecal agar plate cultures of 10 patients in northeastren Thailand and 20 in southern Thailand. Partial ITS1, 5.8S, and ITS2 regions of the ribosomal DNA genes were amplified using PCR. The amplicons were sequenced, aligned, and compared with other hookworm sequences in GenBank database. The results showed that, in Thailand, N. americanus is more prevalent than Ancylostoma spp. and is found in both study areas. Sporadic cases of A. ceylanicum and A. duodenale infection were seen in northeastern Thailand.
authors: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. ; Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Issarapong
authors: I
authors: Phosuk
authors: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. ; Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Pewpan M
authors: PM
authors: Intapan
authors: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. ; Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Tongjit
authors: T
authors: Thanchomnang
authors: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. ; Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Oranuch
authors: O
authors: Sanpool
authors: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. ; Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Penchom
authors: P
authors: Janwan
authors: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. ; Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Porntip
authors: P
authors: Laummaunwai
authors: Department of Medical Technology, School of Allied Health Sciences and Public Health, Walailak University, Thasala, Nakhon Si Thammarat 80160, Thailand.
authors: Witthaya
authors: W
authors: Aamnart
authors: Department of Parasitology, Faculty of Medicine Chiang Mai University, Chiang Mai 50200, Thailand.
authors: Nimit
authors: N
authors: Morakote
authors: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. ; Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Wanchai
authors: W
authors: Maleewong
mesh_terms: D000722:Ancylostoma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D000724:Ancylostomiasis / Q000453:epidemiology*; D000818:Animals; D016000:Cluster Analysis; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005243:Feces / Q000469:parasitology; D006801:Humans; D008969:Molecular Sequence Data; D017221:Necator americanus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D009332:Necatoriasis / Q000453:epidemiology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013785:Thailand / Q000453:epidemiology
chemical_list: D021903:DNA, Ribosomal Spacer; D012340:RNA, Ribosomal, 5.8S
keywords: Ancylostoma ceylanicum; Ancylostoma duodenale; Necator americanus; human; molecular detection