title: Molecular diagnosis of Baylisascaris schroederi infections in giant panda (Ailuropoda melanoleuca) feces using PCR.
abstract: The helminth Baylisascaris schroederi is one of the most harmful parasites infecting giant pandas (Ailuropoda melanoleuca). It is therefore important to develop an exact diagnostic technique to detect this parasite. Using a known number (1, 2, 3, 4, 5, 10, 25, 50, 100) of feces-isolated B. schroederi egg and adult DNA, we developed a PCR to detect a portion of the mitochondrial 12S rRNA and applied it to giant panda fecal samples. The method was sufficiently sensitive to detect B. schroederi DNA from isolated eggs in a fecal sample with a detection threshold of one egg. We detected B. schroederi in 88% of fecal samples, 30% higher than the conventional flotation technique. No cross-reactivity with other common nematode DNA was detected. Our PCR assay may constitute a valuable alternative for the diagnosis of B. schroederi infections.
authors: 1 Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
authors: Xuan
authors: X
authors: Zhou
authors: Hua
authors: H
authors: Yu
authors: Ning
authors: N
authors: Wang
authors: Yue
authors: Y
authors: Xie
authors: Yi-nan
authors: YN
authors: Liang
authors: De-sheng
authors: DS
authors: Li
authors: Cheng-dong
authors: CD
authors: Wang
authors: Si-jie
authors: SJ
authors: Chen
authors: Yu-bo
authors: YB
authors: Yan
authors: Xiao-bin
authors: XB
authors: Gu
authors: Shu-xian
authors: SX
authors: Wang
authors: Xue-rong
authors: XR
authors: Peng
authors: Guang-you
authors: GY
authors: Yang
mesh_terms: D000818:Animals; D017191:Ascaridida Infections / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D001199:Ascaridoidea / Q000235:genetics / Q000302:isolation & purification*; D018509:DNA, Helminth / Q000235:genetics; D005243:Feces / Q000469:parasitology; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D001503:Ursidae
chemical_list: D018509:DNA, Helminth