title: [Quality characteristics of human spermatozoa with hyaluronic acid receptors].
abstract: OBJECTIVE
  To analyze the quality characteristics of human spermatozoa with hyaluronic acid (HA) receptors and search for a new indicator for the assessment of sperm quality.
  
  
  METHODS
  Using sperm-HA binding assay with HA-coated slides, we determined the binding rate of motile sperm with HA receptors and analyzed its correlation with routine semen parameters, sperm membrane function, sperm fertilizing function and diminished/arrested sperm maturation.
  
  
  RESULTS
  The motile sperm with HA binding sites in the acrosomal region showed significantly higher acrosomal integrity ([95.4 +/- 3.9]%) and mitochondrial membrane potential (MMP) ([97.8 +/- 2.1]%) than those in the initial semen ([68.8 +/- 6.2]% and [72.8 +/- 7.4]%) (P < 0.01). The sperm-HA binding scores were correlated mildly with many routine semen parameters (r = 0.195-0.268, P < 0.05), positively with the acrosome reaction level after ionophore challenge (r = 0.666, P < 0.01) and normal sperm morphology (r = 0.417, P < 0.01), and negatively with sperm nucleoprotein immaturation (r = -0.266, P < 0.01), DNA fragmentation (r = -0. 308, P < 0.01) and excessive residual cytoplasm (r = -0.218, P < 0.05).
  
  
  CONCLUSIONS
  Sperm with HA receptors in the acrosomal region exhibit significant advantages in plasma membrane structure, fertilizing potential and maturation. The sperm-HA binding assay, which is based on a relationship between sperm receptors for zona pellucida and HA, is likely to become a new independent indicator for assessing the multiple qualities of spermatozoa.
authors: Laboratory of Reproductive Medicine, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, China. liuyustudio@163.com
authors: Yu
authors: Y
authors: Liu
authors: Medical School of Tongren Polytechnic College, Tongren, Guizhou 554300, China.
authors: Ying
authors: Y
authors: Long
authors: Department of Clinical Laboratory, Nanshan Maternity and Child Healthcare Hospital, Shenzhen, Guangdong 518052, China.
authors: Meng
authors: M
authors: Liu
authors: Laboratory of Reproductive Medicine, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, China.
authors: Xiao-Lan
authors: XL
authors: Chen
authors: Laboratory of Reproductive Medicine, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, China.
authors: Wen-Yuan
authors: WY
authors: Wu
authors: Laboratory of Reproductive Medicine, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, China.
authors: Jin-E
authors: JE
authors: Cheng
mesh_terms: D000177:Acrosome / Q000378:metabolism; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism*; D006820:Hyaluronic Acid; D008297:Male; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism* / Q000502:physiology*
chemical_list: D018960:Hyaluronan Receptors; D006820:Hyaluronic Acid