title: Synthesis, characterization and cytotoxicity of a new palladium(II) complex with a coumarine-derived ligand.
abstract: The new coumarine derivative, 3-(1-(2-hydroxyethylamino)ethylidene)chroman-2,4--dione, and corresponding palladium(II) complex have been synthesized and characterized by microanalysis, infrared, (1)H and (13)C NMR spectroscopy. The proposed structure of the complex was confirmed on the basis of the X-ray structural study. The palladium(II) complex decreased viability of L929 mouse fibrosarcoma, U251 human glioma and B16 mouse melanoma cell lines in a dose dependent manner, while its ligand exhibited no significant cytotoxicity. The cytotoxic effect of the complex was comparable to that of cisplatin, and mediated by apoptosis associated with oxidative stress, mitochondrial depolarization and caspase activation. Therefore, our results indicate that newly synthesized palladium(II) complex might be a potential candidate for anticancer therapy.
authors: College of Health Studies in Ćuprija, Lole Ribara 1/2, 35230 Ćuprija, Serbia.
authors: Dragoslav R
authors: DR
authors: Ilić
authors: Department of Chemistry, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Serbia.
authors: Verica V
authors: VV
authors: Jevtić
authors: Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovića 69, 34000 Kragujevac, Serbia.
authors: Gordana P
authors: GP
authors: Radić
authors: Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Dr. Subotica 1, 11000 Belgrade, Serbia.
authors: Katarina
authors: K
authors: Arsikin
authors: Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Dr. Subotica 1, 11000 Belgrade, Serbia.
authors: Biljana
authors: B
authors: Ristić
authors: Institute for Biological Research, University of Belgrade, Despota Stefana Blvd. 142, 11000 Belgrade, Serbia.
authors: Ljubica
authors: L
authors: Harhaji-Trajković
authors: Department of Chemistry, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Serbia.
authors: Nenad
authors: N
authors: Vuković
authors: Department of Chemistry, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Serbia.
authors: Slobodan
authors: S
authors: Sukdolak
authors: University of Novi Sad, Faculty of Sciences, Department of Physics, Trg Dositeja Obradovića 4, 21000 Novi Sad, Serbia.
authors: Olivera
authors: O
authors: Klisurić
authors: Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Dr. Subotica 1, 11000 Belgrade, Serbia.
authors: Vladimir
authors: V
authors: Trajković
authors: Department of Chemistry, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Serbia. Electronic address: srecko@kg.ac.rs.
authors: Srećko R
authors: SR
authors: Trifunović
mesh_terms: D000818:Animals; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D003374:Coumarins / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D018360:Crystallography, X-Ray; D004354:Drug Screening Assays, Antitumor; D004789:Enzyme Activation; D006801:Humans; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D010165:Palladium / Q000737:chemistry* / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D003374:Coumarins; D008024:Ligands; D017382:Reactive Oxygen Species; D010165:Palladium; C030123:coumarin; D020169:Caspases
keywords: Apoptosis; Coumarine-derived ligand; Crystal structure; Cytotoxicity; Oxidative stress; Palladium(II) complex