title: Serotype determination of adenoviruses in children with respiratory infection.
abstract: OBJECTIVE
  To determine adenovirus serotypes among children with acute respiratory infection (ARI) in Khoozestan province, Iran during years 2010-2011.
  
  
  METHODS
  One hundred sixty three nasopharyngeal swabs were collected from children between 1 and 15 y who were hospitalized for the acute respiratory infection. The viral DNA was extracted from the nasopharyngeal swabs and adenoviruses were detected by Nested PCR. Positive PCR samples were sequenced in order to confirm the adenovirus serotypes.
  
  
  RESULTS
  Out of 163 samples, 30 (18.4 %) were positive for adenoviruses by nested PCR. Twenty two PCR products were sequenced and recognized as Ad5 and Ad2 serotypes including 19 (86.3 %) Ad5 and 3 (13.7 %) Ad2.
  
  
  CONCLUSIONS
  This study reveals that adenoviruses with Ad5 predominance are important cause of respiratory tract infection in children.
authors: Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
authors: Yusuf
authors: Y
authors: Ghasemi
authors: Manoochehr
authors: M
authors: Makvandi
authors: Ali Reza
authors: AR
authors: Samarbafzadeh
authors: Ahmad
authors: A
authors: Nejati
authors: Saeed
authors: S
authors: Najafifard
authors: Niloofar
authors: N
authors: Neisi
authors: Mojtaba
authors: M
authors: Rasti
authors: Kambiz
authors: K
authors: Ahmadi
authors: Ahmad
authors: A
authors: Shamsizadeh
authors: Roya
authors: R
authors: Nikfar
mesh_terms: D000208:Acute Disease; D000256:Adenoviridae / Q000145:classification*; D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D012141:Respiratory Tract Infections / Q000821:virology*; D012703:Serotyping