title: Stability and cytotoxicity of angiotensin-I-converting enzyme inhibitory peptides derived from bovine casein.
abstract: This study investigated the effect of heat treatment combined with acid and alkali on the angiotensin-I-converting enzyme (ACE) inhibitory activity of peptides derived from bovine casein. The free amino group content, color, and cytotoxicity of the peptides were measured under different conditions. When heated at 100 °C in the pH range from 9.0 to 12.0, ACE inhibitory activity was reduced and the appearance of the peptides was significantly darkened. After thermal treatment in the presence of acid and alkali, the free amino group content of ACE inhibitory peptides decreased markedly. High temperature and prolonged heating also resulted in the loss of ACE inhibitory activity, the loss of free amino groups, and the darker coloration of bovine casein-derived peptides. However, ACE inhibitory peptides, within a concentration range of from 0.01 to 0.2 mg/ml, showed no cytotoxicity to Caco-2 and ECV-304 cell lines after heat treatment. This indicated that high temperature and alkaline heat treatment impaired the stability of bovine casein-derived ACE inhibitory peptides.
authors: Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin 150030, China.
authors: Wei
authors: W
authors: Wu
authors: Pan-pan
authors: PP
authors: Yu
authors: Feng-yang
authors: FY
authors: Zhang
authors: Hong-xia
authors: HX
authors: Che
authors: Zhan-mei
authors: ZM
authors: Jiang
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry*; D000818:Animals; D018938:Caco-2 Cells; D002364:Caseins / Q000737:chemistry*; D002417:Cattle; D045744:Cell Line, Tumor; D002470:Cell Survival; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D010455:Peptides / Q000737:chemistry*; D007703:Peptidyl-Dipeptidase A / Q000737:chemistry*
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D002364:Caseins; D010455:Peptides; D007703:Peptidyl-Dipeptidase A