title: Evaluation of the Hologic gen-probe PANTHER, APTIMA Combo 2 assay in a tertiary care teaching hospital.
abstract: OBJECTIVE
  To evaluate the performance of the Hologic Gen-Probe (San Diego, CA) PANTHER system.
  
  
  METHODS
  The performance of PANTHER was compared with the Hologic Gen-Probe TIGRIS and/or Roche (Indianapolis, IN) COBAS AMPLICOR systems through testing of patient specimens and the spiked-urine matrix.
  
  
  RESULTS
  After discrepant resolution, PANTHER demonstrated a 99.3% (95% confidence interval [CI], 96.0%-99.9%) positive and 100% (98.5%-100.0%) negative agreement for Chlamydia trachomatis (CT) and 100% (96.6%-100.0%) positive and 100% (98.6%-100.0%) negative agreement for Neisseria gonorrhoeae (NG) for all male, female, unsexed, and NG-spiked female urine specimens combined. For other specimen types collectively, the PANTHER demonstrated 100% (95% CI, 90.6%-100.0%) positive and 100% (88.3%-100.0%) negative agreement for CT and 90.9% (62.8%-98.4%) positive and 100% (93.5%-100.0%) negative agreement for NG. Analytical sensitivity of the PANTHER in urine matrix was similar to the TIGRIS system.
  
  
  CONCLUSIONS
  The PANTHER system provides an excellent new addition to options for detecting CT and NG, is appropriate for testing urine samples, and will facilitate high-throughput testing in the clinical laboratory.
authors: Beth Israel Deaconess Medical Center, 330 Brookline Ave, YA309, Boston, MA 02215; e-mail: jekirby@bidmcharvard.edu.
authors: Annie
authors: A
authors: Cheng
authors: James E
authors: JE
authors: Kirby
mesh_terms: D000328:Adult; D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D005260:Female; D006069:Gonorrhea / Q000175:diagnosis*; D006784:Hospitals, Teaching; D006801:Humans; D008297:Male; D009344:Neisseria gonorrhoeae / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D063128:Tertiary Health Care; D016482:Urinalysis
chemical_list: D011933:Reagent Kits, Diagnostic
keywords: Chlamydia trachomatis; Neisseria gonorrhoeae; Nucleic acid amplification test; Sexually transmitted diseases