title: [Post-thaw sperm functions of high- and low-fertility sperm donors: a comparative study of 40 cases].
abstract: OBJECTIVE
  To investigate sperm function indexes that can be used to effectively evaluate the sperm donors' fertility so as to select healthy post-thaw semen samples and improve the success rate of assisted reproductive technology.
  
  
  METHODS
  According to the pregnancy outcomes, we divided 40 donor semen samples into a high-fertility group (n = 20) and a low-fertility group (n = 20). We measured and compared the concentration, progressive motility, morphology, acrosome intactness, DNA integrity and mitochondrial membrane potential (MMP) of the post-thaw sperm between the two groups.
  
  
  RESULTS
  There were statistically significant differences between the high- and low-fertility groups in the percentages of morphologically normal sperm ([18.50 +/- 6.10]% vs [14.42 +/- 6.44]%, P < 0.01), acrosome intactness ([86.17 +/- 4.49]% vs [80.04 +/- 7.52]%, P < 0.05) and DNA fragmentation index ([9.21 +/- 3.22]% vs [15.72 +/- 8.20]%, P < 0.05), but not in MMP ([56.75 +/- 18.80]% vs [52.23 +/- 18.86]%, P > 0.05). A significantly positive correlation was found between MMP and sperm motility (r = 0.760, P < 0.05), but not between other sperm functions and sperm concentration and motility.
  
  
  CONCLUSIONS
  Sperm concentration, motility, morphology, acrosome intactness rate and DNA integrity contribute effectively to the evaluation of the fertilization capacity of post-thaw donor semen samples.
authors: Department of Urology, Shanghai Human Sperm Bank, Laboratory of Sperm Development and Genetics, Shanghai Research Institute of Andrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200135, China. chosenlyf@hotmail.com
authors: Yu-Fei
authors: YF
authors: Liu
authors: Department of Urology, Shanghai Human Sperm Bank, Laboratory of Sperm Development and Genetics, Shanghai Research Institute of Andrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200135, China.
authors: Yong
authors: Y
authors: Zhu
authors: Department of Urology, Shanghai Human Sperm Bank, Laboratory of Sperm Development and Genetics, Shanghai Research Institute of Andrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200135, China.
authors: Hui
authors: H
authors: Lu
authors: Department of Urology, Shanghai Human Sperm Bank, Laboratory of Sperm Development and Genetics, Shanghai Research Institute of Andrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200135, China.
authors: Xiao-Rong
authors: XR
authors: Cao
authors: Department of Urology, Shanghai Human Sperm Bank, Laboratory of Sperm Development and Genetics, Shanghai Research Institute of Andrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200135, China.
authors: Feng
authors: F
authors: Yan
authors: Department of Urology, Shanghai Human Sperm Bank, Laboratory of Sperm Development and Genetics, Shanghai Research Institute of Andrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200135, China.
authors: Yong
authors: Y
authors: Liu
authors: Department of Urology, Shanghai Human Sperm Bank, Laboratory of Sperm Development and Genetics, Shanghai Research Institute of Andrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200135, China.
authors: Wen-Bo
authors: WB
authors: Shi
authors: Department of Urology, Shanghai Human Sperm Bank, Laboratory of Sperm Development and Genetics, Shanghai Research Institute of Andrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200135, China.
authors: Can
authors: C
authors: Sun
authors: Department of Urology, Shanghai Human Sperm Bank, Laboratory of Sperm Development and Genetics, Shanghai Research Institute of Andrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200135, China.
authors: Ke-Min
authors: KM
authors: Lu
authors: Department of Urology, Shanghai Human Sperm Bank, Laboratory of Sperm Development and Genetics, Shanghai Research Institute of Andrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200135, China.
authors: Qian
authors: Q
authors: Xiao
authors: Department of Urology, Shanghai Human Sperm Bank, Laboratory of Sperm Development and Genetics, Shanghai Research Institute of Andrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200135, China.
authors: Hong-Liang
authors: HL
authors: Hu
authors: Department of Urology, Shanghai Human Sperm Bank, Laboratory of Sperm Development and Genetics, Shanghai Research Institute of Andrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200135, China.
authors: Zheng
authors: Z
authors: Li
mesh_terms: D000328:Adult; D015925:Cryopreservation; D005260:Female; D005306:Fertilization; D006801:Humans; D008297:Male; D011247:Pregnancy; D012662:Semen Preservation; D013074:Sperm Banks; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*