title: Improvement of the suture-occluded method in rat models of focal cerebral ischemia-reperfusion.
abstract: The aim of the present study was to provide a simple method of establishing a rat model for focal cerebral ischemia-reperfusion (FCIR). The suture-occluded method was used to establish FCIR in male Sprague-Dawley rats. An incision was made over the bifurcation of the common carotid artery (CCA), through which a suture was inserted up to the internal carotid artery (ICA). The suture remained in the skin subsequent to model establishment and was withdrawn to the CCA to enable reperfusion. The reliability of the rat model was assessed via analysis of nerve function, tetrazolium (TTC) staining and pathological examination. Following FCIR in rats, the resulting neurological impairments were observed. TTC staining revealed infarcts and pathological examination revealed typical pathological changes. This modified method was simple, reliable and, therefore, may be used to investigate FCIR.
authors: Department of Neurology, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453100, P.R. China.
authors: Ping
authors: P
authors: Zhang
authors: Department of Neurology, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453100, P.R. China.
authors: Zhen
authors: Z
authors: Huang
authors: Department of Neurology, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453100, P.R. China.
authors: Hai-Qing
authors: HQ
authors: Yan
authors: Department of Neurology, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453100, P.R. China.
authors: Lin-Lin
authors: LL
authors: Su
authors: Department of Neurology, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453100, P.R. China.
authors: Yong-Kun
authors: YK
authors: Gui
authors: Department of Pharmacy, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.
authors: Hai-Xia
authors: HX
authors: Lv
authors: Department of Neurology, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453100, P.R. China.
authors: Bin
authors: B
authors: Zhu
authors: Department of Neurology, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453100, P.R. China.
authors: Tong
authors: T
authors: Li
keywords: focal cerebral ischemia-reperfusion; model; rat