title: Separation and purification of fructooligosaccharides on a zeolite fixed-bed column.
abstract: Fructooligosaccharides (FOS), a well-known prebiotic product, are obtained by enzymatic synthesis and consist of a mixture of mono- and disaccharides. In this work, a methodology for their separation and purification was developed using a zeolite fixed-bed column. The effects of column temperature (40-60°C), eluent flow rate (0.10-0.14 mL/min), injected to bed volume percent ratio (2.6-5.1%), and ethanol concentration in the eluent (40-60%, v/v) were investigated using a fractionary factorial design (2(4-1)), having the separation efficiency and purity as target responses. Additional experiments were performed as well, where the temperature and ethanol concentration were studied in a central composite design (2(2)). In this work, the zeolite fixed-bed column was shown to be a good alternative for FOS purification, allowing a FOS purity of 90% and separation efficiency of 6.86 between FOS and glucose, using an eluent at 45°C with 60% ethanol concentration.
authors: Department of Chemical Engineering, Federal University of Santa Maria, Santa Maria, RS, Brazil.
authors: Raquel Cristine
authors: RC
authors: Kuhn
authors: Marcio Antonio
authors: MA
authors: Mazutti
authors: Francisco
authors: F
authors: Maugeri Filho
mesh_terms: D000327:Adsorption; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D013499:Surface Properties; D013696:Temperature; D017641:Zeolites / Q000737:chemistry*
chemical_list: D009844:Oligosaccharides; C116580:fructooligosaccharide; D017641:Zeolites
keywords: Fixed-bed columns; Fructooligosaccharides; Purity; Separation efficiency; Zeolites