title: Collagen-binding α11 integrin expression in human myometrium and fibroids utilizing a novel RNA in situ probe.
abstract: OBJECTIVE
  Integrin α11β1 is a collagen receptor specific to fibroblasts that regulates myofibroblast differentiation. We sought to determine whether α11β1 is expressed in myometrium and fibroids and whether tissue expression varies.
  
  
  METHODS
  Comparison of α11 in human myometrium and fibroids using Western blot and RNA in situ hybridization.
  
  
  METHODS
  Specimens were obtained from fibroid and myometrium. For Western blots, we used a polyclonal antibody to integrin α11. RNA in situ hybridization was performed using a custom RNA probe for α11 subunit.
  
  
  RESULTS
  Myometrium and fibroids express α11 integrin, with expression 2-fold greater in fibroids. The RNA probe offers a more precise method compared to Western blot using polyclonal human antibody.
  
  
  CONCLUSIONS
  The difference in expression in myometrium and fibroids suggests that α11 is involved in the formation of myofibroblasts and fibroid development.
authors: Department of Obstetrics and Gynecology, Duke University Medical Center, Durham, NC, USA.
authors: Mary
authors: M
authors: Peavey
authors: Department of Obstetrics and Gynecology, Duke University Medical Center, Durham, NC, USA.
authors: Najah
authors: N
authors: Salleh
authors: Department of Obstetrics and Gynecology, Duke University Medical Center, Durham, NC, USA Department of Pathology, Duke University Medical Center, Durham, NC, USA Phyllis.Leppert@duke.edu.
authors: Phyllis
authors: P
authors: Leppert
mesh_terms: D003094:Collagen / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D039001:Integrin alpha Chains / Q000096:biosynthesis* / Q000235:genetics; D007889:Leiomyoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D009215:Myometrium / Q000378:metabolism* / Q000473:pathology; D015347:RNA Probes / Q000235:genetics; D014594:Uterine Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: C121535:ITGA11 protein, human; D039001:Integrin alpha Chains; D015347:RNA Probes; D003094:Collagen
keywords: RNA in situ hybridization; collagen receptor; fibroids; integrin α11β1