title: Brilliant Blue G assisted epiretinal membrane surgery.
abstract: We report intensely staining epiretinal membrane (ERM) with Brilliant Blue G (BBG) under air for two minutes. ERM peeling was performed in 21 cases. After removal of posterior hyaloid, 0.2 mL BBG was first applied on the macula, to stain ERM under air conditions for 2 minutes. Internal limiting membrane (ILM) was intensely stained and peeled in all cases following ERM removal. In 4 cases, the ERM was also observed to be intensely stained with BBG and peeled with an ILM forceps. Postoperatively, the ganglion cell layer thickness was lower in three of the cases, however VA improved in all cases and multifocal electroretinogram revealed no toxicity. Light microscopy of ERM revealed masses of cells whereas; the ILM did not. The increased staining characteristics of ERM and ILM may be resulted from longer contact time of BBG under air pressure.
authors: MD, Turgut Özal University, Medical School, Department of Ophthalmology, Ankara, Turkey.
authors: Yüksel
authors: Y
authors: Totan
authors: MD, Turgut Özal University, Medical School, Department of Ophthalmology, Ankara, Turkey.
authors: Emre
authors: E
authors: Güler
authors: MD, Turgut Özal University, Medical School, Department of Ophthalmology, Ankara, Turkey.
authors: Mehmet Serdar
authors: MS
authors: Dervişoğulları
mesh_terms: D004396:Coloring Agents / Q000627:therapeutic use; D004596:Electroretinography; D019773:Epiretinal Membrane / Q000601:surgery*; D006801:Humans; D007202:Indicators and Reagents / Q000627:therapeutic use*; D008266:Macula Lutea / Q000473:pathology / Q000601:surgery; D012008:Recurrence; D012165:Retinal Ganglion Cells / Q000187:drug effects / Q000502:physiology; D012394:Rosaniline Dyes / Q000627:therapeutic use*; D013194:Staining and Labeling; D014792:Visual Acuity; D014821:Vitrectomy
chemical_list: D004396:Coloring Agents; D007202:Indicators and Reagents; D012394:Rosaniline Dyes; C004692:coomassie Brilliant Blue