title: Overexpression of a novel chrysanthemum SUPERMAN-like gene in tobacco affects lateral bud outgrowth and flower organ development.
abstract: Previous studies have shown that the SUP genes play important roles in flower development and plant growth and morphogenesis. In this study, we isolated and characterized a SUPERMAN-like gene DgSZFP from chrysanthemum. DgSZFP contains one conserved Cys2/His2-type zinc finger motifs in the N-terminal region and an EAR-box in C-terminus. Its expression was significantly higher in nodes, flower buds, disc stamens, and petals than in the other tissues. Overexpression of DgSZFP in tobacco resulted in enhanced branching, reduced plant height, increased the width of petal tubes, produced the staminoid petals and petaloid stamens in flowers, and enhanced the seed weight and size. In addition, DgSZFP-overexpression tobacco plants accumulated high concentrations of cytokinin and chlorophyll. These results suggest that DgSZFP may be the candidate gene for regulating branching and floral organ development in chrysanthemum.
authors: Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, PR China; Department of Ornamental Horticulture, Sichuan Agricultural University, 555 Northeast Road, Wenjiang District, Chengdu, Sichuan 611130, PR China. Electronic address: qinglinliu@126.com.
authors: Qing-Lin
authors: QL
authors: Liu
authors: Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, PR China; Key Lab of Plant Genetics & Molecular Breeding of Department of Life Science, Zhoukou Normal University, East Wenchang Street, Chuanhui District, Zhoukou, Henan 466001, PR China.
authors: Ke-Dong
authors: KD
authors: Xu
authors: Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, PR China.
authors: Nan
authors: N
authors: Ma
authors: Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, PR China. Electronic address: zhaolj5073@sina.com.
authors: Liang-Jun
authors: LJ
authors: Zhao
authors: Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, PR China.
authors: Lin
authors: L
authors: Xi
mesh_terms: D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D002734:Chlorophyll / Q000235:genetics / Q000378:metabolism; D031202:Chrysanthemum / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D003583:Cytokinins / Q000235:genetics / Q000378:metabolism; D035264:Flowers / Q000254:growth & development*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018519:Meristem / Q000254:growth & development; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018547:Plant Stems / Q000254:growth & development*; D030821:Plants, Genetically Modified; D012639:Seeds / Q000254:growth & development; D014026:Nicotiana / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D016335:Zinc Fingers
chemical_list: D029681:Arabidopsis Proteins; D003583:Cytokinins; D010940:Plant Proteins; D014157:Transcription Factors; C096661:superman protein, Arabidopsis; D002734:Chlorophyll
keywords: Branching; Chrysanthemum; DgSZFP; Floral organ development; Transgenic tobacco