title: Relationship between the mobility of phosphocholine headgroup and the protein-liposome interaction: a dielectric spectroscopic study.
abstract: Proteins could affect the headgroup mobility of phospholipid within liposome membranes through the protein-liposome interaction. The variation of headgroup mobility of phospholipid was then investigated by using the dielectric dispersion analysis. The eight proteins (Mw = 4.2-28.7 kDa) were used to investigate the protein-liposome interaction. It has been revealed that the strength of the protein-liposome interaction at 25 °C was linearly correlated with the stability of intramolecular hydrogen bondings of proteins, better than with their hydrophobicity and the surface charge density. Overall, liposomes composed of binary lipid system, appeared to strongly interact with proteins, in contrast to liposomes composed of single, ternary, and quaternary lipid systems. This is probably because liposomes composed of binary lipid system favored to form the microscopic environment where proteins could interact. The present result suggested the heterogeneous phase state of lipid membranes was one of dominant factors for the interaction between proteins and lipid membranes.
authors: Division of Chemical Engineering, Grad. Sch. of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
authors: Toshinori
authors: T
authors: Shimanouchi
authors: Division of Chemical Engineering, Grad. Sch. of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
authors: Noriko
authors: N
authors: Yoshimoto
authors: Division of Chemical Engineering, Grad. Sch. of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
authors: Azusa
authors: A
authors: Hiroiwa
authors: Division of Chemical Engineering, Grad. Sch. of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
authors: Keiichi
authors: K
authors: Nishiyama
authors: Division of Chemical Engineering, Grad. Sch. of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
authors: Keita
authors: K
authors: Hayashi
authors: Division of Chemical Engineering, Grad. Sch. of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan. Electronic address: umakoshi@cheng.es.osaka-u.ac.jp.
authors: Hiroshi
authors: H
authors: Umakoshi
mesh_terms: D058266:Dielectric Spectroscopy; D057927:Hydrophobic and Hydrophilic Interactions; D008081:Liposomes / Q000737:chemistry*; D010767:Phosphorylcholine / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D008081:Liposomes; D011506:Proteins; D010767:Phosphorylcholine
keywords: Hydrogen bond; Lipid composition; Liposome; Protein