title: 2PE-STED microscopy with a single Ti:sapphire laser for reduced illumination.
abstract: We reported a new effective approach to carry out two-photon excitation stimulated emission depletion (2PE-STED) microscopy using a single Ti:sapphire laser system. With an acoustic-optic Bragg cell, the modulated-CW 2PE STED microscope had the benefits of both CW and pulse approaches: lower input power, simple optical scheme and no complicated synchronization. Additionally, it also took advantages of fluorescence yield increasing. The sub-diffraction-limit resolution was demonstrated using ATTO 425-tagged clathrin-coated vesicles.
authors: College of Precision Instrument and Optoelectronics Engineering, Tianjin University, Tianjin, China ; Tianjin Key Laboratory of Biomedical Detecting Techniques and Instruments, Tianjin, China.
authors: Qifeng
authors: Q
authors: Li
authors: College of Precision Instrument and Optoelectronics Engineering, Tianjin University, Tianjin, China.
authors: Yang
authors: Y
authors: Wang
authors: College of Precision Instrument and Optoelectronics Engineering, Tianjin University, Tianjin, China ; Tianjin Key Laboratory of Biomedical Detecting Techniques and Instruments, Tianjin, China.
authors: Da
authors: D
authors: Chen
authors: Department of Chemistry, University of British Columbia, Vancouver, British Columbia, Canada.
authors: Sherry S H
authors: SS
authors: Wu
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry*; D000818:Animals; D016466:CHO Cells; D002966:Clathrin / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D005453:Fluorescence; D007834:Lasers; D008029:Lighting; D008856:Microscopy, Fluorescence / Q000379:methods*; D038761:Photobleaching; D017785:Photons; D013816:Thermodynamics; D013997:Time Factors; D014025:Titanium / Q000737:chemistry*
chemical_list: D002966:Clathrin; D014025:Titanium; D000537:Aluminum Oxide