title: Development of a specimen-sparing multichannel bead assay to detect antiparasite IgG4 for the diagnosis of Schistosoma and Wuchereria infections on the coast of Kenya.
abstract: To better delineate the impact of parasitic coinfection in coastal Kenya, we developed a novel specimen-sparing bead assay using multiplex flow immunoassay (MFI) technology to simultaneously measure serum or plasma immunoglobulin G4 (IgG4) against Brugia malayi antigen (BMA) and Schistosoma haematobium soluble worm antigen (SWAP). Properties of the bead assay were estimated by latent class analysis using data from S. haematobium egg counts/filarial rapid diagnostic cards (RDTs), parasite-specific enzyme-linked immunosorbent assays (ELISAs), and the multichannel IgG4 assay. For schistosomiasis, the bead assay had an estimated sensitivity of 81% and a specificity of 45%, and it was more sensitive than ELISA or urine egg counts for diagnosing infection. For filariasis, it had a sensitivity of 86% and a specificity of 39%, and it was more sensitive than ELISA or RDT. Measuring antibody by MFI is feasible and may provide more accurate epidemiological information than current parasitological tests, especially in the setting of low-intensity infections.
authors: Center for Global Health and Diseases, Case Western Reserve University School of Medicine, Cleveland, Ohio; Division of Vector Borne and Neglected Tropical Diseases, Ministry of Public Health and Sanitation, Nairobi, Kenya; Department of Environmental Sciences, Emory University, Atlanta, Georgia.
authors: Adam S
authors: AS
authors: DuVall
authors: Jessica K
authors: JK
authors: Fairley
authors: Laura
authors: L
authors: Sutherland
authors: Amaya L
authors: AL
authors: Bustinduy
authors: Peter L
authors: PL
authors: Mungai
authors: Eric M
authors: EM
authors: Muchiri
authors: Indu
authors: I
authors: Malhotra
authors: Uriel
authors: U
authors: Kitron
authors: Charles H
authors: CH
authors: King
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D017178:Brugia malayi / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005368:Filariasis / Q000175:diagnosis / Q000276:immunology*; D015200:Fluoroimmunoassay / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007223:Infant; D007630:Kenya; D008297:Male; D008875:Middle Aged; D012548:Schistosoma haematobium / Q000276:immunology*; D012553:Schistosomiasis haematobia / Q000175:diagnosis / Q000276:immunology*; D013048:Specimen Handling / Q000379:methods; D055815:Young Adult
chemical_list: D000909:Antibodies, Helminth; D007074:Immunoglobulin G