title: Distribution and genetic diversity of the microorganisms in the biofilter for the simultaneous removal of arsenic, iron and manganese from simulated groundwater.
abstract: A biofilter was developed in this study, which showed an excellent performance with the simultaneous removal of AsIII from 150 to 10mg L(-1) during biological iron and manganese oxidation. The distribution and genetic diversity of the microorganisms along the depth of the biofilter have been investigated using DGGE. Results suggested that Iron oxidizing bacteria (IOB, such as Gallionella, Leptothrix), Manganese oxidizing bacteria (MnOB, such as Leptothrix, Pseudomonas, Hyphomicrobium, Arthrobacter) and AsIII-oxidizing bacteria (AsOB, such as Alcaligenes, Pseudomonas) are dominant in the biofilter. The spatial distribution of IOB, MnOB and AsOB at different depths of the biofilter determined the removal zone of FeII, MnII and AsIII, which site at the depths of 20, 60 and 60cm, respectively, and the corresponding removal efficiencies were 86%, 84% and 87%, respectively. This process shows great potential to the treatment of groundwater contaminated with iron, manganese and arsenic due to its stable performance and significant cost-savings.
authors: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
authors: Liu
authors: L
authors: Yang
authors: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
authors: Xiangkun
authors: X
authors: Li
authors: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
authors: Zhaorui
authors: Z
authors: Chu
authors: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
authors: Yuhui
authors: Y
authors: Ren
authors: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China. Electronic address: yongtc@163.com.
authors: Jie
authors: J
authors: Zhang
mesh_terms: D001151:Arsenic / Q000302:isolation & purification*; D001419:Bacteria / Q000235:genetics*; D001673:Biodegradation, Environmental; D018441:Biofilms; D019149:Bioreactors / Q000382:microbiology; D058645:Denaturing Gradient Gel Electrophoresis; D005374:Filtration / Q000295:instrumentation*; D014644:Genetic Variation; D060587:Groundwater / Q000737:chemistry* / Q000382:microbiology; D007501:Iron / Q000302:isolation & purification*; D008345:Manganese / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D014874:Water Pollutants, Chemical / Q000302:isolation & purification
chemical_list: D014874:Water Pollutants, Chemical; D008345:Manganese; D007501:Iron; D001151:Arsenic
keywords: Biofilter; DGGE; Groundwater; Iron and manganese; Simultaneous removal of arsenic