title: Cyclic mononucleotides modulate potassium and calcium flux responses to H2O2 in Arabidopsis roots.
abstract: Cyclic mononucleotides are messengers in plant stress responses. Here we show that hydrogen peroxide (H2O2) induces rapid net K(+)-efflux and Ca(2+)-influx in Arabidopsis roots. Pre-treatment with either 10μM cAMP or cGMP for 1 or 24h does significantly reduce net K(+)-leakage and Ca(2+)-influx, and in the case of the K(+)-fluxes, the cell permeant cyclic mononucleotides are more effective. We also examined the effect of 10μM of the cell permeant 8-Br-cGMP on the Arabidopsis microsomal proteome and noted a specific increase in proteins with a role in stress responses and ion transport, suggesting that cGMP is sufficient to directly and/or indirectly induce complex adaptive changes to cellular stresses induced by H2O2.
authors: Biological and Environmental Sciences & Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia.
authors: Natalia Maria
authors: NM
authors: Ordoñez
authors: Biological and Environmental Sciences & Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia.
authors: Claudius
authors: C
authors: Marondedze
authors: Bioscience Core Facility, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia.
authors: Ludivine
authors: L
authors: Thomas
authors: Department of Applied Biology, University of Perugia, Borgo XX Giugno, 74, 06121 Perugia, Italy.
authors: Stefania
authors: S
authors: Pasqualini
authors: School of Agricultural Science, University of Tasmania, Private Bag 54, Hobart, Tasmania 7001, Australia.
authors: Lana
authors: L
authors: Shabala
authors: School of Agricultural Science, University of Tasmania, Private Bag 54, Hobart, Tasmania 7001, Australia.
authors: Sergey
authors: S
authors: Shabala
authors: Biological and Environmental Sciences & Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia. Electronic address: christoph.gehring@KAUST.edu.sa.
authors: Chris
authors: C
authors: Gehring
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D000242:Cyclic AMP / Q000502:physiology*; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000494:pharmacology / Q000502:physiology*; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D018517:Plant Roots / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism*; D020543:Proteome / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D020543:Proteome; D015124:8-Bromo Cyclic Adenosine Monophosphate; C016276:8-bromocyclic GMP; D006861:Hydrogen Peroxide; D000242:Cyclic AMP; D006152:Cyclic GMP; D011188:Potassium; D002118:Calcium
keywords: Arabidopsis thaliana; Cyclic mononucleotide; Hydrogen peroxide; Ion flux; Plant stress; Proteomic; cAMP; cGMP