title: High concentrations of genistein exhibit pro-oxidant effects in primary muscle cells through mechanisms involving 5-lipoxygenase-mediated production of reactive oxygen species.
abstract: Genistein, a typical soy isoflavone, is an important antioxidant for improving human health and animal production but the compound possesses some pro-oxidant potential. In order to explore the latter, the dose-response relationship of various concentrations of genistein on both cellular proliferation and the redox system were examined. The proliferation of primary muscle cells was promoted by a low concentration of genistein but was inhibited by high concentrations, which also enhanced lipid oxidation and suppressed membrane fluidity. By selecting a high concentration (200 μM) as a pro-oxidant treatment, the mechanism underlying the pro-oxidant function of genistein was then explored. The generation of intracellular reactive oxygen species (ROS) was stimulated by 200 μM genistein, with inhibited expression of NADPH oxidase 4 and cyclooxygenase 1 and 2 as well as increased activity of the glutathione redox system. The cellular expression of 5-lipoxygenase, however, was up-regulated by 200 μM genistein and the addition of 5-lipoxygenase inhibitor (Zileuton) decreased genistein-induced intracellular ROS level, close to that from the addition of the ROS scavenger, N-acetylcysteine. It is concluded that higher concentrations of genistein exert pro-oxidant potential in the primary muscle cells through enhancing ROS production in a 5-lipoxygenase-dependent manner.
authors: Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, PR China; The Key Laboratory of Animal Nutrition and Feed Science (South China) of Ministry of Agriculture, Guangzhou, PR China; State Key Laboratory of Livestock and Poultry Breeding, Guangzhou, PR China; Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou, PR China; Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou, PR China. Electronic address: cwei010230@163.com.
authors: Wei
authors: W
authors: Chen
authors: Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, PR China; The Key Laboratory of Animal Nutrition and Feed Science (South China) of Ministry of Agriculture, Guangzhou, PR China; State Key Laboratory of Livestock and Poultry Breeding, Guangzhou, PR China; Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou, PR China; Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou, PR China. Electronic address: lyc0123@tom.com.
authors: Ying Cai
authors: YC
authors: Lin
authors: Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, PR China; The Key Laboratory of Animal Nutrition and Feed Science (South China) of Ministry of Agriculture, Guangzhou, PR China; State Key Laboratory of Livestock and Poultry Breeding, Guangzhou, PR China; Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou, PR China; Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou, PR China. Electronic address: xianyongm@gmail.com.
authors: Xian Yong
authors: XY
authors: Ma
authors: Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, PR China; The Key Laboratory of Animal Nutrition and Feed Science (South China) of Ministry of Agriculture, Guangzhou, PR China; State Key Laboratory of Livestock and Poultry Breeding, Guangzhou, PR China; Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou, PR China; Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou, PR China. Electronic address: jiangz41@163.com.
authors: Zong Yong
authors: ZY
authors: Jiang
authors: Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, PR China; The Key Laboratory of Animal Nutrition and Feed Science (South China) of Ministry of Agriculture, Guangzhou, PR China; State Key Laboratory of Livestock and Poultry Breeding, Guangzhou, PR China; Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou, PR China; Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou, PR China. Electronic address: 619836899@qq.com.
authors: Si Ping
authors: SP
authors: Lan
mesh_terms: D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism*; D001483:Base Sequence; D002478:Cells, Cultured; D017931:DNA Primers; D019833:Genistein / Q000494:pharmacology*; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D016133:Polymerase Chain Reaction; D017382:Reactive Oxygen Species / Q000378:metabolism* / Q000494:pharmacology*; D013552:Swine
chemical_list: D017931:DNA Primers; D017382:Reactive Oxygen Species; D019833:Genistein; D001094:Arachidonate 5-Lipoxygenase
keywords: 5-Lipoxygenase; Genistein; Primary muscle cells; Pro-oxidant; Reactive oxygen species