title: [Establishment of mouse lens epithelial cell lines with the genotype of Hsf4-/-].
abstract: OBJECTIVE
  To establish mouse lens epithelial cell lines with the genotype of Hsf4-/-.
  
  
  METHODS
  The expended mouse lens epithelial cells, which were generated from P6 Hsf4-deficient mouse lens epithelia, were immortalized with SV40-T-antigen and named MLEC/Hsf4-/- cell. The expression of alpha A-crystallin was immunoblotted. Hsf4b cDNA was reconstituted by transiently transfection.
  
  
  RESULTS
  The SV40-immortalized cells were in adherent growth mode with spindle morphology, pseudopodia, clear nuclear boundary membrane and cytoplasm translucent. Immunoblotting results indicated that the lens biomarker protein alpha A-crystallin was expressed in MLEC/Hsf4-/- cells. Reconstitution of Hsf4b into MLEC/Hsf4-/- cells upregulated the expression of Hsp25.
  
  
  CONCLUSIONS
  The SV40-immortalized MLEC/Hsf4-/- cells have the lens epithelial characteristics and could be used as a tool for studying the signal transduction in vitro.
authors: Key Laboratory of Cell and Molecular Immunology, Medical College of Henan University, Kaifeng 475000, China.
authors: Jun
authors: J
authors: Zhang
authors: Zeng-yi
authors: ZY
authors: Ma
authors: Yue-ling
authors: YL
authors: Wang
authors: Ji-yan
authors: JY
authors: Wang
authors: Email: hyz@henu.edu.cn.
authors: Yan-zhong
authors: YZ
authors: Hu
mesh_terms: D000818:Animals; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics; D004847:Epithelial Cells / Q000166:cytology*; D000076249:Heat Shock Transcription Factors; D007908:Lens, Crystalline / Q000166:cytology*; D051379:Mice; D014157:Transcription Factors / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D000076249:Heat Shock Transcription Factors; C484462:Hsf4 protein, mouse; D014157:Transcription Factors