title: Stimulation of cerebral angiogenesis by gene delivery.
abstract: Angiogenesis, an important process for long term neurological recovery, could be induced by ischemic brain injury. In this chapter, we describe a system to deliver adeno-associated viral (AAV) vector-mediated gene therapy for ischemic stroke. This includes the methods to construct, produce, and purify an AAV vector expressing target gene and an approach to quantify the number of microvessels and capillary density with synchrotron radiation angiography (SRA) imaging.
authors: Neuroscience and Neuroengineering Research Center, Med-X Research Institute and School of Biomedical Engineering, Shanghai, China.
authors: Yaohui
authors: Y
authors: Tang
authors: Yaning
authors: Y
authors: Li
authors: Xiaojie
authors: X
authors: Lin
authors: Peng
authors: P
authors: Miao
authors: Yongting
authors: Y
authors: Wang
authors: Guo-Yuan
authors: GY
authors: Yang
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000503:physiopathology; D002560:Cerebrovascular Circulation; D000229:Dependovirus / Q000235:genetics; D005822:Genetic Vectors; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D018919:Neovascularization, Physiologic / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014161:Transduction, Genetic; D014776:Virus Cultivation