title: In vitro remineralizing effect of fluoride varnishes containing sodium trimetaphosphate.
abstract: This study analyzed the effects of fluoride varnishes supplemented with sodium trimetaphosphate (TMP) on the remineralization of caries-like lesions in vitro. Bovine enamel discs were selected through surface hardness (SH) and caries-like lesions were induced. SH was again determined and the blocks were divided into 7 experimental groups (n = 24/group): placebo (no fluoride or TMP), 5% TMP, 2.5% NaF, 2.5% NaF/5% TMP, 5% NaF, 5% NaF/5% TMP and commercial formulation (Duraphat™, 5% NaF), following a blind protocol. Discs were treated with the varnishes and kept in a remineralizing solution for 4 h and a demineralizing solution for 2 h. Varnishes were then removed and half of the discs were used for analysis of loosely (CaF2) and firmly bound fluoride. The remaining discs were submitted to a pH-cycling regimen for 6 days. The percentage of surface hardness recovery (%SHR), cross-sectional hardness (ΔKHN) and enamel CaF2 and fluoride were determined. Data were analyzed by ANOVA and by Student-Newman-Keuls' test (p < 0.05). A dose-response relationship was observed between fluoride concentrations in the varnishes and %SHR. The 5% TMP varnish led to %SHR similar to that obtained for the placebo. When TMP was used in association with fluoride, however, significantly higher %SHR were observed in comparison with their counterparts without TMP. Moreover, ΔKHN obtained for the 5% NaF/5% TMP was significantly lower among all groups tested. Higher concentrations of CaF2 and fluoride were observed for Duraphat and 5% NaF, followed by 5% NaF/5% TMP, 2.5% NaF and 2.5% NaF/5% TMP (p < 0.05). It was concluded that the supplementation of fluoride varnishes with TMP leads to enhanced remineralizing effect of artificial caries lesions in vitro.
authors: M M
authors: MM
authors: Manarelli
authors: A C B
authors: AC
authors: Delbem
authors: T M T
authors: TM
authors: Lima
authors: F C N
authors: FC
authors: Castilho
authors: J P
authors: JP
authors: Pessan
mesh_terms: D019412:Anatomy, Cross-Sectional; D000818:Animals; D001706:Biopsy; D002124:Calcium Fluoride / Q000032:analysis; D002327:Cariostatic Agents / Q000494:pharmacology*; D002417:Cattle; D003743:Dental Enamel / Q000737:chemistry / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005460:Fluorides, Topical / Q000494:pharmacology* / Q000627:therapeutic use; D006244:Hardness; D006863:Hydrogen-Ion Concentration; D010919:Placebos; D011122:Polyphosphates / Q000494:pharmacology*; D012969:Sodium Fluoride / Q000627:therapeutic use; D013997:Time Factors; D017001:Tooth Demineralization / Q000503:physiopathology; D014089:Tooth Remineralization / Q000379:methods*
chemical_list: D002327:Cariostatic Agents; D005460:Fluorides, Topical; D010919:Placebos; D011122:Polyphosphates; C023807:sodium fluoride topical preparation; D012969:Sodium Fluoride; C009293:trimetaphosphoric acid; D002124:Calcium Fluoride