title: In vivo and in situ tracking cancer chemotherapy by highly photostable NIR fluorescent theranostic prodrug.
abstract: In vivo monitoring of the biodistribution and activation of prodrugs is urgently required. Near infrared (NIR) fluorescence-active fluorophores with excellent photostability are preferable for tracking drug release in vivo. Herein, we describe a NIR prodrug DCM-S-CPT and its polyethylene glycol-polylactic acid (PEG-PLA) loaded nanoparticles as a potent cancer therapy. We have conjugated a dicyanomethylene-4H-pyran derivative as the NIR fluorophore with camptothecin (CPT) as the anticancer drug using a disulfide linker. In vitro experiments verify that the high intracellular glutathione (GSH) concentrations in tumor cells cause cleavage of the disulfide linker, resulting in concomitantly the active drug CPT release and significant NIR fluorescence turn-on with large Stokes shift (200 nm). The NIR fluorescence of DCM-S-CPT at 665 nm with fast response to GSH can act as a direct off-on signal reporter for the GSH-activatable prodrug. Particularly, DCM-S-CPT possesses much better photostability than ICG, which is highly desirable for in situ fluorescence-tracking of cancer chemotherapy. DCM-S-CPT has been successfully utilized for in vivo and in situ tracking of drug release and cancer therapeutic efficacy in living animals by NIR fluorescence. DCM-S-CPT exhibits excellent tumor-activatable performance when intravenously injected into tumor-bearing nude mice, as well as specific cancer therapy with few side effects. DCM-S-CPT loaded in PEG-PLA nanoparticles shows even higher antitumor activity than free CPT, and is also retained longer in the plasma. The tumor-targeting ability and the specific drug release in tumors make DCM-S-CPT as a promising prodrug, providing significant advances toward deeper understanding and exploration of theranostic drug-delivery systems.
authors: Key Laboratory for Advanced Materials and Institute of Fine Chemicals, Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology , Shanghai 200237, China.
authors: Xumeng
authors: X
authors: Wu
authors: Xuanrong
authors: X
authors: Sun
authors: Zhiqian
authors: Z
authors: Guo
authors: Jianbin
authors: J
authors: Tang
authors: Youqing
authors: Y
authors: Shen
authors: Tony D
authors: TD
authors: James
authors: He
authors: H
authors: Tian
authors: Weihong
authors: W
authors: Zhu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004355:Drug Stability; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism / Q000627:therapeutic use*; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D007259:Infrared Rays; D042541:Intracellular Space / Q000378:metabolism; D019344:Lactic Acid / Q000737:chemistry; D051379:Mice; D057054:Molecular Imaging; D053758:Nanoparticles / Q000737:chemistry; D009369:Neoplasms / Q000175:diagnosis* / Q000188:drug therapy*; D011091:Polyesters; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011355:Prodrugs / Q000737:chemistry / Q000378:metabolism / Q000627:therapeutic use*
chemical_list: D005456:Fluorescent Dyes; D011091:Polyesters; D011108:Polymers; D011355:Prodrugs; D019344:Lactic Acid; D011092:Polyethylene Glycols; C033616:poly(lactide); D005978:Glutathione