title: Dynamics of +1 ribosomal frameshifting.
abstract: It has been well characterized that the amino acid starvation can induce +1 frameshifting. However, how the +1 frameshifting occurs has not been fully understood. Here, taking Escherichia coli RF2 programmed frameshifting as an example we present systematical analysis of the +1 frameshifting that could occur during every state-transition step in elongation phase of protein synthesis, showing that the +1 frameshifting can occur only during the period after deacylated tRNA dissociation from the posttranslocation state and before the recognition of the next "hungry" codon. The +1 frameshifting efficiency is theoretically studied, with the simple analytical solutions showing that the high efficiency is almost solely due to the occurrence of ribosome pausing which in turn results from the insufficient RF2. The analytical solutions also provide a consistent explanation of a lot of independent experimental data.
authors: Key Laboratory of Soft Matter Physics and Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: pxie@iphy.ac.cn.
authors: Ping
authors: P
authors: Xie
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000378:metabolism; D018965:Frameshifting, Ribosomal; D055641:Mathematical Concepts; D008954:Models, Biological; D008957:Models, Genetic; D010441:Peptide Chain Elongation, Translational; D010454:Peptide Termination Factors / Q000378:metabolism; D020871:RNA Stability; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D012343:RNA, Transfer / Q000235:genetics / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D010454:Peptide Termination Factors; D012329:RNA, Bacterial; C489006:prfB protein, E coli; D012343:RNA, Transfer
keywords: Ribosome; Ribosome pausing; Translation elongation; Translational regulation; Virus