title: Three new clerodane diterpenes from Polyalthia longifolia var. pendula.
abstract: Three new clerodane diterpenes, (4→2)-abeo-cleroda-2,13E-dien-2,14-dioic acid (1), (4→2)-abeo-2,13-diformyl-cleroda-2,13E-dien-14-oic acid (2), and 16(R&amp;S)- methoxycleroda-4(18),13-dien-15,16-olide (3), were isolated from the unripe fruit of Polyalthia longifolia var. pendula (Annonaceae) together with five known compounds (4-8). The structures of all isolates were determined by spectroscopic analysis. The anti-inflammatory activity of the isolates was evaluated by testing their inhibitory effect on NO production in LPS-stimulated RAW 264.7 macrophages. Among the isolated compounds, 16-hydroxycleroda-3,13-dien-15,16-olide (6) and 16-oxocleroda-3,13-dien-15-oic acid (7) showed promising NO inhibitory activity at 10 µg/mL, with 81.1% and 86.3%, inhibition, respectively.
authors: Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan. thwu@isca.vghks.gov.tw.
authors: Tung-Ho
authors: TH
authors: Wu
authors: Graduate Institute of Pharmaceutical Chemistry, China Medical University, Taichung 404, Taiwan. vivinecheng001@yahoo.com.tw.
authors: Yung-Yi
authors: YY
authors: Cheng
authors: Proteomics Core Laboratory, Department of Medical Research, China Medical University Hospital, Taichung 404, Taiwan. cjchen@mail.cmu.edu.tw.
authors: Chao-Jung
authors: CJ
authors: Chen
authors: Department of Agricultural Chemistry, National Taiwan University, Taipei 106, Taiwan. nglt97@ntu.edu.tw.
authors: Lean-Teik
authors: LT
authors: Ng
authors: Graduate Institute of Pharmaceutical Chemistry, China Medical University, Taichung 404, Taiwan. lichen1973@gmail.com.
authors: Li-Chen
authors: LC
authors: Chou
authors: Graduate Institute of Pharmaceutical Chemistry, China Medical University, Taichung 404, Taiwan. ljhuang@mail.cmu.edu.tw.
authors: Li-Jiau
authors: LJ
authors: Huang
authors: National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan. tony_chen72001@yahoo.com.tw.
authors: Yung-Husan
authors: YH
authors: Chen
authors: Graduate Institute of Pharmaceutical Chemistry, China Medical University, Taichung 404, Taiwan. sckuo@mail.cmu.edu.tw.
authors: Sheng-Chu
authors: SC
authors: Kuo
authors: Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, Ain-Shams University, Organization of African Unity Street, Abassia, Cairo 11566, Egypt. mohamed.elshazly@pharm.asu.edu.eg.
authors: Mohamed
authors: M
authors: El-Shazly
authors: Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan. yachwu@mail.cmu.edu.tw.
authors: Yang-Chang
authors: YC
authors: Wu
authors: Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan. aaronfrc@kmu.edu.tw.
authors: Fang-Rong
authors: FR
authors: Chang
authors: Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan. ccliaw@mail.nsysu.edu.tw.
authors: Chih-Chuang
authors: CC
authors: Liaw
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic; D045785:Diterpenes, Clerodane / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy*; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D031044:Polyalthia / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D000972:Antineoplastic Agents, Phytogenic; D045785:Diterpenes, Clerodane