title: Biochemical responses to the toxicity of the biocide abamectin on the freshwater snail Physa acuta.
abstract: The toxic effects of abamectin (ABM), an anthelmintic drug, on the snail, Physa Acuta, and the biochemical responses to the exposure stress were evaluated. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), acetylcholinesterase (AChE), and nitric oxide synthase (NOS), and the contents of malondialdehyde (MDA) were determined in snail soft tissues (head, foot, visceral mass, and the mantle) for up to 96h of exposure to 3.4, 9.6, 19.2, or 27.4μgL(-1) of ABM. The results showed that SOD and GST activities were promoted by ABM-exposure at the earlier periods of treatment (12-48h) while these activites were inhibited at the end of test. The tendency of CAT activity was similar to that of SOD, but it increased at the end of test. MDA levels of the snail soft tissues increased in all treatment groups, including the recovery group, indicating that lipid peroxidation occurred in snail soft tissues. ABM-exposure inhibited AChE activity. However, NOS activities increased by ABM-exposure. In addition, activities of antioxidant enzymes and AChE from the snail soft tissues resumed the normal levels after 96h of recovery period, but MDA level did not attain the original level. This study provides information on the biochemical mechanism of ABM toxicity on the snail.
authors: College of Life Science, Henan Normal University, Xinxiang, Henan 453007, China.
authors: Junguo
authors: J
authors: Ma
authors: College of Life Science, Henan Normal University, Xinxiang, Henan 453007, China.
authors: Chune
authors: C
authors: Zhou
authors: College of Life Science, Henan Normal University, Xinxiang, Henan 453007, China.
authors: Yao
authors: Y
authors: Li
authors: College of Life Science, Henan Normal University, Xinxiang, Henan 453007, China. Electronic address: lixiaoyu65@263.net.
authors: Xiaoyu
authors: X
authors: Li
mesh_terms: D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects; D004798:Enzymes / Q000378:metabolism; D005618:Fresh Water; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D012908:Snails / Q000187:drug effects*; D013995:Time; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D004798:Enzymes; D014874:Water Pollutants, Chemical; D008315:Malondialdehyde; C048324:abamectin; D007559:Ivermectin
keywords: Abamectin; Acetylcholinesterase; Antioxidant enzymes; Lipid peroxidation; Nitric oxide synthase; Physa acuta