title: Optimization of complex fermentation media for glucose oxidase production using statistical approach.
abstract: Production cost of enzyme is largely determined by the type of the strain and raw material used to propagate the strain. Hence, selection of the strain and raw materials is crucial in enzyme production. For Glucose oxidase (GOx), previous studies showed Aspergillus terreus UniMAP AA-1 offers a better alternative to the existing sources. Thus, a lower production cost could be logically anticipated by growing the strain in a cheaper complex media such as molasses. In this work, sugar cane molasses, supplemented with urea and carbonate salt and a locally isolated strain Aspergillus terreus UniMAP AA-1 were used to produce a crude GOx enzyme in a small scale. A statistical optimization approach namely Response Surface Methodology (RSM) was used to optimize the media components for highest GOx activity. It was found that the highest GOx activity was achieved using a combination of molasses, carbonate salt and urea at concentration 32.51, 4.58 and 0.93% (w/v), respectively. This study provides an alternative optimized media conditions for GOx production using locally available raw materials.
authors: School of Bioprocess Engineering, Universiti Malaysia Perlis, Kompleks Pusat Pengajian Jejawi 3, 02600 Arau, Perlis, Malaysia.
authors: Ahmad Anas Nagoor
authors: AA
authors: Gunny
authors: School of Bioprocess Engineering, Universiti Malaysia Perlis, Kompleks Pusat Pengajian Jejawi 3, 02600 Arau, Perlis, Malaysia.
authors: Dachyar
authors: D
authors: Arbain
authors: School of Bioprocess Engineering, Universiti Malaysia Perlis, Kompleks Pusat Pengajian Jejawi 3, 02600 Arau, Perlis, Malaysia.
authors: Logachanthirika
authors: L
authors: Sithamparam
mesh_terms: D001230:Aspergillus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000096:biosynthesis*; D002119:Calcium Carbonate / Q000378:metabolism; D005285:Fermentation; D005942:Gluconates / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005949:Glucose Oxidase / Q000096:biosynthesis*; D007218:Industrial Microbiology / Q000379:methods*; D007700:Kinetics; D007783:Lactones / Q000378:metabolism; D015233:Models, Statistical; D008965:Molasses; D015203:Reproducibility of Results; D017550:Spectroscopy, Fourier Transform Infrared; D013379:Substrate Specificity; D014508:Urea / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D005942:Gluconates; D007783:Lactones; D014508:Urea; D005949:Glucose Oxidase; D002119:Calcium Carbonate; D005947:Glucose; C010730:beta-glucono-1,5-lactone