title: Pretreatment of mice with oligonucleotide prop5 protects them from influenza virus infections.
abstract: Influenza A virus is a successful parasite and requires host factors to complete its life cycle. Prop5 is an antisense oligonucleotide, targeting programmed cell death protein 5 (PDCD5). In this study, we tested the antiviral activity of prop5 against mouse-adapted A/FM/1/47 strain of influenza A virus in a mouse model. Prop5 intranasally administered the mice at dosages of 10 and 20 mg/kg/d at 24 h and 30 min before infection, provided 80% and 100% survival rates and prolonged mean survival days in comparison with influenza virus-infected mice (both p < 0.01). Moreover, viral titres in mice pretreated with prop5, at dose of 10 and 20 mg/kg/d, had declined significantly on day two, four, and six post-infection compared with the yields in infected mice (p < 0.05 or p < 0.01); lung index in mice pretreated with prop5 (20 mg/kg/d) had been inhibited on day six post-infection (p < 0.05). Western blotting and immunohistochemistry showed that prop5 could down-regulate the PDCD5 protein expression levels in lung tissues of infected mice. These data indicate that antisense oligonucleotide prop5 is a promising drug for prophylaxis and control influenza virus infections and provides an insight into the host-pathogen interaction.
authors: Beijing University of Technology, No.100, Pingleyuan, Chaoyang District, Beijing 100124, China. caaslk@163.com.
authors: Kang
authors: K
authors: Li
authors: Beijing Institute of Radiation Medicine, No.27, Taiping Road, Haidian District, Beijing 100850, China. zhouzhe@bmi.ac.cn.
authors: Zhe
authors: Z
authors: Zhou
authors: Beijing Institute of Radiation Medicine, No.27, Taiping Road, Haidian District, Beijing 100850, China. kings108@163.com.
authors: Yu Ou
authors: YO
authors: Wang
authors: Beijing Institute of Radiation Medicine, No.27, Taiping Road, Haidian District, Beijing 100850, China. liu458868056@126.com.
authors: Juan
authors: J
authors: Liu
authors: Beijing Institute of Radiation Medicine, No.27, Taiping Road, Haidian District, Beijing 100850, China. xuehaibaozong@163.com.
authors: Hai Bao
authors: HB
authors: Zhao
authors: Beijing Institute of Radiation Medicine, No.27, Taiping Road, Haidian District, Beijing 100850, China. yangjing@bmi.ac.cn.
authors: Jing
authors: J
authors: Yang
authors: Beijing Institute of Radiation Medicine, No.27, Taiping Road, Haidian District, Beijing 100850, China. sqwang@nic.bmi.ac.cn.
authors: Sheng Qi
authors: SQ
authors: Wang
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage*; D051017:Apoptosis Regulatory Proteins / Q000037:antagonists & inhibitors*; D004195:Disease Models, Animal; D006801:Humans; D009980:Influenza A virus / Q000187:drug effects*; D008168:Lung / Q000821:virology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors*; D020319:Oligodeoxyribonucleotides, Antisense / Q000008:administration & dosage*; D009841:Oligonucleotides / Q000008:administration & dosage*; D009975:Orthomyxoviridae; D009976:Orthomyxoviridae Infections / Q000517:prevention & control*; D016019:Survival Analysis; D016896:Treatment Outcome; D019562:Viral Load
chemical_list: D000998:Antiviral Agents; D051017:Apoptosis Regulatory Proteins; D009363:Neoplasm Proteins; D020319:Oligodeoxyribonucleotides, Antisense; D009841:Oligonucleotides; C486488:Pdcd5 protein, mouse