title: Isolation, preliminary characterization and hepatoprotective activity of polysaccharides from Tamarindus indica L.
abstract: Polysaccharide was isolated from Tamarindus indica L. (TIP) and was characterized in terms of moisture and ash content, pH, water holding capacity, particle size, tapped density, bulk density, carr's index, Hausners ratio, angle of repose, content of glucose, uronic acid and sulfate. Morphological, spectral (UV-vis, FTIR) and DSC thermal analysis reveals polysaccharide nature of the isolated starch. DPPH radical scavenging activity of TIP shows RSA comparable to that of silymarin. Hepatoprotective potential of TIP in terms of biochemical parameters, SGOT, SGPT, ALP and BRN were significantly increased (P<0.05) and reduction of serum Total protein in the group of rats given thioacetamide (100mg/kg s.c.). Histopathology reveals that TIP under antagonize the effect of thioacetamide by acting, either as membrane stabilizer, thereby preventing the distortion of the cellular ionic environment associated with thioacetamide intoxication, or by preventing interaction of thioacetamide with the transcriptional machinery of the cells.
authors: Institute of Pharmaceutical Sciences, Guru Ghasidas Vishwavidyalaya, Bilaspur C.G-495009, India. Electronic address: samalpharmacology@rediffmail.com.
authors: Predeep Kumar
authors: PK
authors: Samal
authors: Institute of Pharmaceutical Sciences, Guru Ghasidas Vishwavidyalaya, Bilaspur C.G-495009, India.
authors: Jawahar Singh
authors: JS
authors: Dangi
mesh_terms: D000818:Animals; D002152:Calorimetry, Differential Scanning; D005260:Female; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008111:Liver Function Tests; D008297:Male; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D029861:Tamarindus / Q000737:chemistry*; D013853:Thioacetamide / Q000633:toxicity
chemical_list: D011134:Polysaccharides; D013853:Thioacetamide
keywords: Hepatoprotective; Polysacharide; SGOT; SGPT; Tamarindus indicus