title: In situ gelling dorzolamide loaded chitosan nanoparticles for the treatment of glaucoma.
abstract: The most important risk associated with glaucoma is the onset and progression of intraocular pressure. The objective of this study was to formulate in situ gel of chitosan nanoparticles to enhance the bioavailability and efficacy of dorzolamide in the glaucoma treatment. Optimized nanoparticles were spherical in shape (particle size: 164 nm) with a loading efficiency of 98.1%. The ex vivo release of the optimized in situ gel nanoparticle formulation showed a sustained drug release as compared to marketed formulation. The gamma scintigraphic study of prepared in situ nanoparticle gel showed good corneal retention compared to marketed formulation. HET-CAM assay of the prepared formulation scored 0.33 in 5 min which indicates the non-irritant property of the formulation. Thus in situ gel of dorzolamide hydrochloride loaded nanoparticles offers a more intensive treatment of glaucoma and a better patient compliance as it requires fewer applications per day compared to conventional eye drops.
authors: Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
authors: Shefali
authors: S
authors: Katiyar
authors: Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
authors: Jayamanti
authors: J
authors: Pandit
authors: Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
authors: Rabi S
authors: RS
authors: Mondal
authors: Institute of Nuclear Medicine and Allied Sciences, Lucknow Road, Timarpur, Delhi 110054, India.
authors: Anil K
authors: AK
authors: Mishra
authors: Institute of Nuclear Medicine and Allied Sciences, Lucknow Road, Timarpur, Delhi 110054, India.
authors: Krishna
authors: K
authors: Chuttani
authors: Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
authors: Mohd
authors: M
authors: Aqil
authors: Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
authors: Asgar
authors: A
authors: Ali
authors: Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India. Electronic address: yas2312003@yahoo.com.
authors: Yasmin
authors: Y
authors: Sultana
mesh_terms: D000818:Animals; D002152:Calorimetry, Differential Scanning; D002257:Carbonic Anhydrase Inhibitors / Q000737:chemistry / Q000627:therapeutic use*; D048271:Chitosan / Q000737:chemistry*; D005901:Glaucoma / Q000188:drug therapy*; D006041:Goats; D006801:Humans; D066298:In Vitro Techniques; D053758:Nanoparticles / Q000737:chemistry / Q000627:therapeutic use*; D011877:Radionuclide Imaging; D013449:Sulfonamides / Q000737:chemistry / Q000627:therapeutic use*; D013876:Thiophenes / Q000737:chemistry / Q000627:therapeutic use*; D014961:X-Ray Diffraction
chemical_list: D002257:Carbonic Anhydrase Inhibitors; D013449:Sulfonamides; D013876:Thiophenes; D048271:Chitosan; C062765:dorzolamide
keywords: Chitosan nanoparticles; Dorzolamide; Glaucoma; In situ gel