title: Coronin 6 regulates acetylcholine receptor clustering through modulating receptor anchorage to actin cytoskeleton.
abstract: The maintenance of a high density of neurotransmitter receptors at the postsynaptic apparatus is critical for efficient neurotransmission. Acetylcholine receptors (AChRs) are neurotransmitter receptors densely packed on the postsynaptic muscle membrane at the neuromuscular junction (NMJ) via anchoring onto the actin cytoskeletal network. However, how the receptor-associated actin is coordinately regulated is not fully understood. We report here that Coronin 6, a newly identified member of the coronin family, is highly enriched at adult NMJs and regulates AChR clustering through modulating the interaction between receptors and the actin cytoskeletal network. Experiments with cultured myotubes reveal that Coronin 6 is important for both agrin- and laminin-induced AChR clustering. Furthermore, Coronin 6 forms a complex with AChRs and actin in a manner dependent on its C-terminal region and a conserved Arg(29) residue at the N terminus, both of which are critical for the cytoskeletal anchorage of AChRs. Importantly, in vivo knockdown of Coronin 6 in mouse skeletal muscle fibers leads to destabilization of AChR clusters. Together, these findings demonstrate that Coronin 6 is a critical regulator of AChR clustering at the postsynaptic region of the NMJs through modulating the receptor-anchored actin cytoskeleton.
authors: JNU-HKUST Joint Laboratory for Neuroscience and Innovative Drug Research, Jinan University, Guangzhou 510632, Guangdong, China, Division of Life Science and State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China, and Guangdong Key Laboratory of Brain Science, Disease and Drug Development, HKUST Shenzhen Research Institute, Shenzhen, Guangdong, China.
authors: Yuewen
authors: Y
authors: Chen
authors: Fanny C F
authors: FC
authors: Ip
authors: Lei
authors: L
authors: Shi
authors: Zhe
authors: Z
authors: Zhang
authors: Huibin
authors: H
authors: Tang
authors: Yu Pong
authors: YP
authors: Ng
authors: Wen-Cai
authors: WC
authors: Ye
authors: Amy K Y
authors: AK
authors: Fu
authors: Nancy Y
authors: NY
authors: Ip
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D003599:Cytoskeleton / Q000378:metabolism*; D018274:Electroporation; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008840:Microfilament Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009469:Neuromuscular Junction / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011950:Receptors, Cholinergic / Q000378:metabolism*
chemical_list: D000199:Actins; D008840:Microfilament Proteins; D011950:Receptors, Cholinergic; C072061:coronin proteins
keywords: Coronin 6; acetylcholine; actin cytoskeleton; actin-binding proteins