title: Association of 'Candidatus Liberibacter solanacearum' with a vegetative disorder of celery in Spain and development of a real-time PCR method for its detection.
abstract: A new symptomatology was observed in celery (Apium graveolens) in Villena, Spain in 2008. Symptomatology included an abnormal amount of shoots per plant and curled stems. These vegetative disorders were associated with 'Candidatus Liberibacter solanacearum' and not with phytoplasmas. Samples from plant sap were immobilized on membranes based on the spot procedure and tested using a newly developed real-time polymerase chain reaction assay to detect 'Ca. L. solanacearum'. Then, a test kit was developed and validated by intralaboratory assays with an accuracy of 100%. Bacterial-like cells with typical morphology of 'Ca. Liberibacter' were observed using electron microscopy in celery plant tissues. A fifth haplotype of 'Ca. L. solanacearum', named E, was identified in celery and in carrot after analyzing partial sequences of 16S and 50S ribosomal RNA genes. From our results, celery (family Apiaceae) can be listed as a new natural host of this emerging bacterium.
authors: Gabriela R
authors: GR
authors: Teresani
authors: Edson
authors: E
authors: Bertolini
authors: Ana
authors: A
authors: Alfaro-Fernández
authors: Carmen
authors: C
authors: Martínez
authors: Francisco André Ossamu
authors: FA
authors: Tanaka
authors: Elliot W
authors: EW
authors: Kitajima
authors: Montserrat
authors: M
authors: Roselló
authors: Susana
authors: S
authors: Sanjuán
authors: Juan Carlos
authors: JC
authors: Ferrándiz
authors: María M
authors: MM
authors: López
authors: Mariano
authors: M
authors: Cambra
authors: María Isabel
authors: MI
authors: Font
mesh_terms: D000077102:Apium / Q000382:microbiology* / Q000648:ultrastructure; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D018552:Daucus carota / Q000382:microbiology; D006239:Haplotypes; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D018520:Plant Shoots / Q000382:microbiology / Q000648:ultrastructure; D018547:Plant Stems / Q000382:microbiology / Q000648:ultrastructure; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012230:Rhizobiaceae / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D017422:Sequence Analysis, DNA; D013030:Spain; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D004275:DNA, Ribosomal