title: Impairment of kindling development in phospholipase Cγ1 heterozygous mice.
abstract: OBJECTIVE
  Elucidating molecular mechanisms underlying limbic epileptogenesis may reveal novel targets for preventive therapy. Studies of TrkB mutant mice led us to hypothesize that signaling through a specific phospholipase (PLC), PLCγ1, promoted development of kindling.
  
  
  METHODS
  To test this hypothesis, we examined the development of kindling in PLCγ1 heterozygous mice. We also examined the cellular and subcellular location of PLCγ1 in adult wild-type mice.
  
  
  RESULTS
  The development of kindling was impaired in PLCγ1 heterozygous mice compared to wild-type controls. PLCγ1 immunoreactivity was localized to the soma and dendrites of both excitatory and inhibitory neurons in the hippocampus of adult mice.
  
  
  CONCLUSIONS
  This study implicates PLCγ1 signaling as the dominant pathway by which TrkB activation promotes limbic epileptogenesis. Its cellular localization places PLCγ1 in a position to modify the efficacy of both excitatory and inhibitory synaptic transmission. These findings advance PLCγ1 as a novel target for therapies aimed at preventing temporal lobe epilepsy induced by status epilepticus.
authors: Department of Medicine (Neurology), Duke University Medical Center, Durham, North Carolina, U.S.A.
authors: Xiao Ping
authors: XP
authors: He
authors: Renren
authors: R
authors: Wen
authors: James O
authors: JO
authors: McNamara
mesh_terms: D000818:Animals; D006579:Heterozygote; D006624:Hippocampus / Q000737:chemistry* / Q000201:enzymology* / Q000473:pathology; D007696:Kindling, Neurologic / Q000235:genetics* / Q000473:pathology; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D051966:Phospholipase C gamma / Q000032:analysis* / Q000235:genetics*; D012640:Seizures / Q000235:genetics / Q000473:pathology; D015398:Signal Transduction / Q000502:physiology
chemical_list: D051966:Phospholipase C gamma; C117399:Plcg1 protein, mouse
keywords: Epilepsy; PLCγ1; Seizure; TrkB