title: The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants.
abstract: Clathrin-mediated endocytosis is the major mechanism for eukaryotic plasma membrane-based proteome turn-over. In plants, clathrin-mediated endocytosis is essential for physiology and development, but the identification and organization of the machinery operating this process remains largely obscure. Here, we identified an eight-core-component protein complex, the TPLATE complex, essential for plant growth via its role as major adaptor module for clathrin-mediated endocytosis. This complex consists of evolutionarily unique proteins that associate closely with core endocytic elements. The TPLATE complex is recruited as dynamic foci at the plasma membrane preceding recruitment of adaptor protein complex 2, clathrin, and dynamin-related proteins. Reduced function of different complex components severely impaired internalization of assorted endocytic cargoes, demonstrating its pivotal role in clathrin-mediated endocytosis. Taken together, the TPLATE complex is an early endocytic module representing a unique evolutionary plant adaptation of the canonical eukaryotic pathway for clathrin-mediated endocytosis.
authors: Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors: Astrid
authors: A
authors: Gadeyne
authors: Max-Planck-Institut für Moleculare Planzenphysiologie, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany.
authors: Clara
authors: C
authors: Sánchez-Rodríguez
authors: Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors: Steffen
authors: S
authors: Vanneste
authors: Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors: Simone
authors: S
authors: Di Rubbo
authors: Max-Planck-Institut für Moleculare Planzenphysiologie, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany.
authors: Henrik
authors: H
authors: Zauber
authors: Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors: Kevin
authors: K
authors: Vanneste
authors: Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors: Jelle
authors: J
authors: Van Leene
authors: Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors: Nancy
authors: N
authors: De Winne
authors: Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors: Dominique
authors: D
authors: Eeckhout
authors: Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors: Geert
authors: G
authors: Persiau
authors: Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors: Eveline
authors: E
authors: Van De Slijke
authors: Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors: Bernard
authors: B
authors: Cannoot
authors: Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors: Leen
authors: L
authors: Vercruysse
authors: Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA.
authors: Jonathan R
authors: JR
authors: Mayers
authors: Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium; Institute of Science and Technology (IST Austria), Am Campus 1, 3400 Klosterneuburg, Austria.
authors: Maciek
authors: M
authors: Adamowski
authors: Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium; Institute of Science and Technology (IST Austria), Am Campus 1, 3400 Klosterneuburg, Austria.
authors: Urszula
authors: U
authors: Kania
authors: Max-Planck-Institut für Moleculare Planzenphysiologie, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany.
authors: Matthias
authors: M
authors: Ehrlich
authors: Max-Planck-Institut für Moleculare Planzenphysiologie, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany; Institute of Biotechnology (IBT), University of Vilnius, V. Graiciuno 8, 02242 Vilnius, Lithuania.
authors: Alois
authors: A
authors: Schweighofer
authors: Laboratory of Cell Biology, Wageningen University, Droevendaalsesteeg 1, 6708PB Wageningen, The Netherlands.
authors: Tijs
authors: T
authors: Ketelaar
authors: Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors: Steven
authors: S
authors: Maere
authors: Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA.
authors: Sebastian Y
authors: SY
authors: Bednarek
authors: Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium; Institute of Science and Technology (IST Austria), Am Campus 1, 3400 Klosterneuburg, Austria.
authors: Jiří
authors: J
authors: Friml
authors: Department of Medical Protein Research, VIB, A. Baertsoenkaai 3, 9000 Ghent, Belgium; Department of Biochemistry, Ghent University, 9000 Ghent, Belgium.
authors: Kris
authors: K
authors: Gevaert
authors: Department of Biology, Center for Proteome Analysis and Mass Spectrometry, University of Antwerp, 2020 Antwerp, Belgium; Flemish Institute for Technological Research (VITO), 2400 Mol, Belgium.
authors: Erwin
authors: E
authors: Witters
authors: Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors: Eugenia
authors: E
authors: Russinova
authors: Max-Planck-Institut für Moleculare Planzenphysiologie, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany. Electronic address: persson@mpimp-golm.mpg.de.
authors: Staffan
authors: S
authors: Persson
authors: Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors: Geert
authors: G
authors: De Jaeger
authors: Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium. Electronic address: daniel.vandamme@psb.vib-ugent.be.
authors: Daniël
authors: D
authors: Van Damme
mesh_terms: D033962:Adaptor Protein Complex 2 / Q000378:metabolism; D017360:Arabidopsis / Q000166:cytology* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002966:Clathrin / Q000378:metabolism*; D034281:Dynamins / Q000378:metabolism; D004705:Endocytosis; D046912:Multiprotein Complexes / Q000378:metabolism
chemical_list: D033962:Adaptor Protein Complex 2; D029681:Arabidopsis Proteins; D002966:Clathrin; D046912:Multiprotein Complexes; D034281:Dynamins