title: Analysis of genetically modified organisms by pyrosequencing on a portable photodiode-based bioluminescence sequencer.
abstract: A portable bioluminescence analyser for detecting the DNA sequence of genetically modified organisms (GMOs) was developed by using a photodiode (PD) array. Pyrosequencing on eight genes (zSSIIb, Bt11 and Bt176 gene of genetically modified maize; Lectin, 35S-CTP4, CP4EPSPS, CaMV35S promoter and NOS terminator of the genetically modified Roundup ready soya) was successfully detected with this instrument. The corresponding limit of detection (LOD) was 0.01% with 35 PCR cycles. The maize and soya available from three different provenances in China were detected. The results indicate that pyrosequencing using the small size of the detector is a simple, inexpensive, and reliable way in a farm/field test of GMO analysis.
authors: Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China; Department of Pharmacology, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China.
authors: Qinxin
authors: Q
authors: Song
authors: Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China.
authors: Guijiang
authors: G
authors: Wei
authors: Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China; Department of Pharmacology, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China. Electronic address: ghzhou@nju.edu.cn.
authors: Guohua
authors: G
authors: Zhou
mesh_terms: D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D030841:Food, Genetically Modified; D059014:High-Throughput Nucleotide Sequencing / Q000295:instrumentation / Q000379:methods*; D049449:Luminescence; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D013025:Glycine max / Q000737:chemistry / Q000235:genetics*; D003313:Zea mays / Q000737:chemistry / Q000235:genetics*
chemical_list: D018744:DNA, Plant
keywords: Bt-11 and Bt-176 maize; Genetically modified organisms (GMO); Portable bioluminescence analyser; Pyrosequencing; Roundup ready soya