title: Biological characteristics and probiotic effect of Leuconostoc lactis strain isolated from the intestine of black porgy fish.
abstract: A strain of lactic acid bacteria, Leuconostoc lactis, was isolated from the intestinal tract of black porgy, Sparus macrocephalus, and identified by conventional biochemical characteristics and 16S rDNA gene sequence analysis. The isolated strain had the ability of bile tolerance and resistance to low pH, and survived well in the trypsinase and pepsin solution. But the highly concentrated dose of trypsinase and pepsin affect the viability of the isolated strain. The isolate was resistant to several antibiotics, including Cephalothin, Ceftriaxone, Imipenem and Tobramycin. The isolate could auto-aggregate itself and coaggregate with other bacteria in vitro. The autoaggregation percentage increased to 23.29% after 20 h of incubation. The percentage of coaggregation were respectively 31.21%, 29.44%, 10.74%, 16.49%, 24.36%, 24.41% and 20.99% for Vibrio parahaemolyticus, Listeria monocytogenes, Escherichia coli O157, Salmonella typhimurium, Shigella, Staphylococcus aureus and Proteusbacillus vulgaris after 20 h incubation of a mixed suspension. The supernatant of the strain inhibited the growth of several pathogens, such as V.parahaemolyticus, Vibrio harveyi, Vibrio alginolyticus, Staphylococcus aureus, Escherichia coli O157, Salmonella typhimurium, Bacillus subtilis, Proteusbacillus vulgaris and Shigella. These results indicated that the isolate, Leuconostoc lactis, might be an attractive candidate for perspectival strain for probiotics in marine aquaculture.
authors: College of Life Sciences, China Jiliang University, Hangzhou, China.
authors: Wei
authors: W
authors: Zhang
authors: College of Life Sciences, China Jiliang University, Hangzhou, China.
authors: Mingqi
authors: M
authors: Liu
authors: College of Life Sciences, China Jiliang University, Hangzhou, China.
authors: Xianjun
authors: X
authors: Dai
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000898:Antibiosis; D001422:Bacterial Adhesion; D015373:Bacterial Typing Techniques; D001647:Bile Acids and Salts / Q000633:toxicity; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D007422:Intestines / Q000382:microbiology*; D007934:Leuconostoc / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology*; D050296:Microbial Viability / Q000187:drug effects; D010434:Pepsin A / Q000378:metabolism; D010473:Perciformes / Q000382:microbiology*; D010802:Phylogeny; D019936:Probiotics / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014357:Trypsin / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001647:Bile Acids and Salts; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D014357:Trypsin; D010434:Pepsin A
keywords: Leuconostoc lactis; biological characteristic; black porgy; lactic acid bacteria; probiotic