title: Giant vesicles functionally expressing membrane receptors for an insect pheromone.
abstract: To date, biochemical approaches to membrane receptors have been limited to the following methods: knockout or overexpression of membrane receptors by gene introduction and genome engineering or extraction of membrane receptor-surfactant complexes from innate cells and their introduction into model biomembranes. Here, we describe the development of a third method involving gene expression using cell-free in situ protein synthesis inside model biomembrane capsules. We verified this method by synthesizing olfactory receptors from the silkmoth Bombyx mori inside giant vesicles and found that they were excited in the presence of their ligand the Bombyx mori sex pheromone.
authors: Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, Chiba University, 1-33 Yayoi, Inage, Chiba, Chiba 263-8522, Japan. fujinami@faculty.chiba-u.jp.
authors: Satoshi
authors: S
authors: Hamada
authors: Masashi
authors: M
authors: Tabuchi
authors: Taro
authors: T
authors: Toyota
authors: Takeshi
authors: T
authors: Sakurai
authors: Tomohiro
authors: T
authors: Hosoi
authors: Tomonori
authors: T
authors: Nomoto
authors: Kei
authors: K
authors: Nakatani
authors: Masanori
authors: M
authors: Fujinami
authors: Ryohei
authors: R
authors: Kanzaki
mesh_terms: D000818:Animals; D012831:Bombyx; D005233:Fatty Alcohols / Q000378:metabolism; D049452:Green Fluorescent Proteins; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D018035:Receptors, Odorant / Q000235:genetics / Q000378:metabolism*; D043563:Receptors, Pheromone / Q000235:genetics / Q000378:metabolism*; D012724:Sex Attractants / Q000378:metabolism
chemical_list: D005233:Fatty Alcohols; D019476:Insect Proteins; D018035:Receptors, Odorant; D043563:Receptors, Pheromone; D012724:Sex Attractants; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C006787:bombykol