title: [Effect and mechanisms of TET on human gastric carcinoma cell line SGC7901 and SGC7901/ADR].
abstract: OBJECTIVE
  To investigate the effect of tetrandrine (TET) on zinc finger protein 139 (ZNF139) and multidrug resistance (MDR) of human gastric carcinoma cell lines and possible mechanisms.
  
  
  METHODS
  Cultured SGC7901 and SGC7901/ADR were treated with TET (0.5, 1.0, 1.5, 2.0, and 2.5 microg/mL), then inhibition rates were measured by MTT assay in vitro. The expressions of ZNF139, MRP-1, MDR1, and GST-pi were detected by RT-PCR. The correlation between ZNF139 and each multidrug resistance factor was analyzed using Spearman correlation analysis, and the coefficient correlation was calculated.
  
  
  RESULTS
  The inhibition rate of TET (< or = 2.0 microg/mL) for SGC7901 and SGC7901/ADR was less than 10% with MTT assay. Expressions of ZNF139, MRP-1, MDR1, and GST-pi mRNA were higher in SGC7901/ADR than in SGC7901 (all P < 0.05). The expressions of ZNF139, MRP-1, MDR1, and GST--pi were down-regulated in SGC7901/ADR cells efficiently (all P < 0.01). Positive correlation existed between ZNF139 and MRP-1, ZNF139 and MDR1 before treated by TET in SGC7901/ADR, and this relationship also existed in SGC7901/ADR cells after treated by TET (all P < 0.05).
  
  
  CONCLUSIONS
  TET could achieve MDR reversion in gastric cancer cells by down-regulating the expression of ZNF139, MRP-1, and MDR1.
authors: Department of General Surgery, Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang 050011, China. li_yong_hbth@126.com
authors: Yong
authors: Y
authors: Li
authors: Department of General Surgery, Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang 050011, China.
authors: Qun
authors: Q
authors: Zhao
authors: Department of General Surgery, Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang 050011, China.
authors: Bi-Bo
authors: BB
authors: Tan
authors: Department of General Surgery, Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang 050011, China.
authors: Li-Qiao
authors: LQ
authors: Fan
authors: Department of General Surgery, Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang 050011, China.
authors: Qing-Wei
authors: QW
authors: Liu
authors: Department of General Surgery, Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang 050011, China.
authors: Zhi-Kai
authors: ZK
authors: Jiao
authors: Department of General Surgery, Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang 050011, China.
authors: Xue-Feng
authors: XF
authors: Zhao
authors: Department of General Surgery, Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang 050011, China.
authors: Ying-Jie
authors: YJ
authors: Hao
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000378:metabolism; D044182:Benzylisoquinolines / Q000494:pharmacology*; D045744:Cell Line, Tumor; D018432:Drug Resistance, Multiple / Q000187:drug effects* / Q000235:genetics; D019008:Drug Resistance, Neoplasm / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000378:metabolism; D013274:Stomach Neoplasms / Q000378:metabolism*; D016335:Zinc Fingers / Q000235:genetics*
chemical_list: D018435:ATP Binding Cassette Transporter, Subfamily B; D044182:Benzylisoquinolines; D051741:Kruppel-Like Transcription Factors; D027425:ATP-Binding Cassette, Sub-Family C Proteins; C513055:ABCB1 protein, human; C000590232:ZKSCAN1 protein, human; C009438:tetrandrine; C421109:multidrug resistance-associated protein 1