title: The role of CD44-hyaluronic acid interaction in exogenous mesenchymal stem cells homing to rat remnant kidney.
abstract: OBJECTIVE
  The aim of our study was to reveal the role of CD44-Hyaluronic acid (HA) in the homing and improving renal function of systemically transplanted MSCs in chronic renal failure.
  
  
  METHODS
  First, a remnant kidney model was established in rats and the expression of HA was determined using immunohistochemistry (IHC) and western blotting. Next, chemotaxis assay using flow cytometry, and cell migration assay of MSCs were performed in vitro. Then, MSCs were transplanted into rats, thus, sprague-Dawley (SD) rats were randomly divided into sham group, 5/6 nephrectomy (5/6 Nx) group, MSC group and MSC/Anti-CD44 group (n = 8 for all groups). Migration of MSCs to the kidney in these rats was assessed by using cell tracking experiments, and tissue damage was evaluated by morphological analysis using Masson's trichrome staining and periodic acid Schiff staining.
  
  
  RESULTS
  HA was significantly observed in 5/6 Nx group, but not in sham group. Meanwhile, HA was discovered induced MSCs migration remarkably (p < 0.05) and anti-CD44 antibody inhibited the migration significantly (p < 0.05) in vitro. In vivo, the GFP-MSCs were observed in MSC group and the cells reduced in MSC/Anti-CD44 groups, especially, in the tubulointerstitium.
  
  
  CONCLUSIONS
  Our findings reveal that CD44-HA has the potential to induce MSCs homing to injured tissue, while its effect on the ability of MSCs, improving tissue function, is not significant.
authors: Departments of Nephrology, The First Affiliated Hospital, China Medical University, Shenyang, Liaoning, China.
authors: Xiao-Hui
authors: XH
authors: Bian
authors: Guang-Yu
authors: GY
authors: Zhou
authors: Li-Ning
authors: LN
authors: Wang
authors: Jian-Fei
authors: JF
authors: Ma
authors: Qiu-Ling
authors: QL
authors: Fan
authors: Na
authors: N
authors: Liu
authors: Yu
authors: Y
authors: Bai
authors: Wei
authors: W
authors: Guo
authors: Yan-Qiu
authors: YQ
authors: Wang
authors: Guang-Ping
authors: GP
authors: Sun
authors: Ping
authors: P
authors: He
authors: Xu
authors: X
authors: Yang
authors: Xue-Song
authors: XS
authors: Su
authors: Feng
authors: F
authors: Du
authors: Gui-Feng
authors: GF
authors: Zhao
authors: Jia-Ning
authors: JN
authors: Miao
authors: Li
authors: L
authors: Ma
authors: Li-Qiang
authors: LQ
authors: Zheng
authors: De-Tian
authors: DT
authors: Li
authors: Jiang-Min
authors: JM
authors: Feng
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D003404:Creatinine / Q000097:blood; D018960:Hyaluronan Receptors / Q000494:pharmacology*; D006820:Hyaluronic Acid / Q000494:pharmacology*; D007668:Kidney / Q000166:cytology* / Q000187:drug effects; D007672:Kidney Cortex / Q000166:cytology; D007674:Kidney Diseases / Q000097:blood / Q000503:physiopathology / Q000628:therapy*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D009392:Nephrectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D014508:Urea / Q000097:blood
chemical_list: D018960:Hyaluronan Receptors; D014508:Urea; D006820:Hyaluronic Acid; D003404:Creatinine