title: Inhibitory effect of plant Manilkara subsericea against biological activities of Lachesis muta snake venom.
abstract: Snake venom is composed of a mixture of substances that caused in victims a variety of pathophysiological effects. Besides antivenom, literature has described plants able to inhibit injuries and lethal activities induced by snake venoms. This work describes the inhibitory potential of ethanol, hexane, ethyl acetate, or dichloromethane extracts and fractions from stem and leaves of Manilkara subsericea against in vivo (hemorrhagic and edema) and in vitro (clotting, hemolysis, and proteolysis) activities caused by Lachesis muta venom. All the tested activities were totally or at least partially reduced by M. subsericea. However, when L. muta venom was injected into mice 15 min first or after the materials, hemorrhage and edema were not inhibited. Thus, M. subsericea could be used as antivenom in snakebites of L. muta. And, this work also highlights Brazilian flora as a rich source of molecules with antivenom properties.
authors: Laboratório de Venenos e Toxinas de Animais e Avaliação de Inibidores, Departamento de Biologia Celular e Molecular, Instituto de Biologia, Universidade Federal Fluminense, Outeiro de São João Batista, 3 º andar, Sala 310, 24020-141 Niterói, RJ, Brazil.
authors: Eduardo Coriolano
authors: EC
authors: De Oliveira
authors: Programa de Pós-Graduação em Ciências Aplicadas a Produtos para a Saúde, Faculdade de Farmácia, Universidade Federal Fluminense, 24220-900 Niterói, RJ, Brazil ; Programa de Pós-Graduação em Biotecnologia Vegetal, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, 21941-599 Rio de Janeiro, RJ, Brazil.
authors: Caio Pinho
authors: CP
authors: Fernandes
authors: Centro de Pesquisa e Desenvolvimento, Fundação Ezequiel Dias, 30510-010 Belo Horizonte, MG, Brazil.
authors: Eladio Flores
authors: EF
authors: Sanchez
authors: Programa de Pós-Graduação em Ciências Aplicadas a Produtos para a Saúde, Faculdade de Farmácia, Universidade Federal Fluminense, 24220-900 Niterói, RJ, Brazil ; Programa de Pós-Graduação em Biotecnologia Vegetal, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, 21941-599 Rio de Janeiro, RJ, Brazil.
authors: Leandro
authors: L
authors: Rocha
authors: Laboratório de Venenos e Toxinas de Animais e Avaliação de Inibidores, Departamento de Biologia Celular e Molecular, Instituto de Biologia, Universidade Federal Fluminense, Outeiro de São João Batista, 3 º andar, Sala 310, 24020-141 Niterói, RJ, Brazil.
authors: André Lopes
authors: AL
authors: Fuly
mesh_terms: D000818:Animals; D000997:Antivenins / Q000008:administration & dosage* / Q000737:chemistry; D001938:Brazil; D032129:Manilkara / Q000737:chemistry; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D012910:Snake Venoms / Q000633:toxicity*; D017819:Viperidae
chemical_list: D000997:Antivenins; D010936:Plant Extracts; D012910:Snake Venoms