title: Genotoxic and oxidative responses in coelomocytes of Eisenia fetida and Hediste diversicolor exposed to lipid-coated CdSe/ZnS quantum dots and CdCl2.
abstract: The emerging of Quantum Dots utilization in industrial or medicinal fields involved a potentially increase of these nanoparticles in environment. In this work, the genotoxic (comet assay) and oxidative effects (SOD activity, TBARS) of functionalized-QDs and cadmium chloride were investigated on Hediste diversicolor and Eisenia fetida coelomocytes. Results demonstrated that functionalized-QDs (QDNs) and cadmium chloride induced DNA damages through different mechanisms that depended on the nano- or ionic nature of Cd. The minimal genotoxic concentrations for H. diversicolor (<0.001ng/g for QDNs and CdCl2 ) were lower than for E. fetida (between 0.01 and 0.1 ng/g for QDNs, and between 0.001 and 0.01 ng/g for CdCl2 ). These results showed that H. diversicolor was more sensitive than E. fetida. The two contaminants had a low impact on the oxidative stress markers.
authors: Laboratoire de Mutagenèse Environnementale, Institut Méditerranéen de Biodiversité et d'Ecologie, Faculté de Pharmacie, Aix-Marseille Université, 27 Bd Jean Moulin, 13385, Marseille, Cedex 05, France.
authors: Gladys
authors: G
authors: Saez
authors: Laboratoire de Mutagenèse Environnementale, Institut Méditerranéen de Biodiversité et d'Ecologie, Faculté de Pharmacie, Aix-Marseille Université, 27 Bd Jean Moulin, 13385, Marseille, Cedex 05, France.
authors: Mélanie
authors: M
authors: Aye
authors: Laboratoire de Mutagenèse Environnementale, Institut Méditerranéen de Biodiversité et d'Ecologie, Faculté de Pharmacie, Aix-Marseille Université, 27 Bd Jean Moulin, 13385, Marseille, Cedex 05, France.
authors: Michel
authors: M
authors: De Meo
authors: INSERM, U869, ARNA Laboratory, F-33000, Bordeaux, France.|University of Bordeaux, ARNA Laboratory, F-33000, Bordeaux, France.
authors: Ahissan
authors: A
authors: Aimé
authors: INSERM, U869, ARNA Laboratory, F-33000, Bordeaux, France.|University of Bordeaux, ARNA Laboratory, F-33000, Bordeaux, France.
authors: Isabelle
authors: I
authors: Bestel
authors: INSERM, U869, ARNA Laboratory, F-33000, Bordeaux, France.|University of Bordeaux, ARNA Laboratory, F-33000, Bordeaux, France.
authors: Philippe
authors: P
authors: Barthélémy
authors: Laboratoire de Mutagenèse Environnementale, Institut Méditerranéen de Biodiversité et d'Ecologie, Faculté de Pharmacie, Aix-Marseille Université, 27 Bd Jean Moulin, 13385, Marseille, Cedex 05, France.
authors: Carole
authors: C
authors: Di Giorgio
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D019256:Cadmium Chloride / Q000633:toxicity*; D020552:Comet Assay; D004249:DNA Damage; D007962:Leukocytes / Q000187:drug effects*; D009153:Mutagens / Q000633:toxicity*; D053758:Nanoparticles / Q000633:toxicity; D009835:Oligochaeta; D018384:Oxidative Stress / Q000187:drug effects; D011077:Polychaeta; D045663:Quantum Dots / Q000633:toxicity*; D012643:Selenium / Q000633:toxicity*; D013440:Sulfides / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances; D017967:Zinc Compounds / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D009153:Mutagens; D013440:Sulfides; D017392:Thiobarbituric Acid Reactive Substances; D017967:Zinc Compounds; D013482:Superoxide Dismutase; D012643:Selenium; D019256:Cadmium Chloride; C031238:zinc sulfide
keywords: cadmium; genotoxicity; invertebrates; quantum dots