title: Differential accumulation of γ-aminobutyric acid in elicited cells of two rice cultivars showing contrasting sensitivity to the blast pathogen.
abstract: Intracellular free amino acid pools were quantified in suspension cultured cells of a blast-sensitive and a blast-resistant rice genotype at increasing times after treatment with Magnaporthe oryzae cell wall hydrolysates. Besides some expected variations in free phenylalanine, a remarkable early increase of γ-aminobutyric acid (GABA) levels was evident in both cultivars. Glutamate decarboxylase activity and protein levels were unaffected. GABA homeostasis was recovered in the sensitive cultivar 48 h after the treatment. In contrast, a further GABA accumulation and a general increase of most amino acids was found at this later stage in the resistant genotype, which showed a larger decrease in cell viability as a consequence of elicitor addition. Data support a recently hypothesised role of GABA metabolism in the plant response to fungal pathogens.
authors: Department of Life Science and Biotechnology, University of Ferrara, Ferrara, Italy.
authors: G
authors: G
authors: Forlani
authors: M
authors: M
authors: Bertazzini
authors: S
authors: S
authors: Giberti
mesh_terms: D002478:Cells, Cultured; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006706:Homeostasis; D020082:Magnaporthe / Q000502:physiology*; D012275:Oryza / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D005680:gamma-Aminobutyric Acid
keywords: Elicitors; Italian rice genotypes; Magnaporthe oryzae; free amino acids; γ-aminobutyric acid