title: Protein profile of the seminal plasma of collared peccaries (Pecari tajacu Linnaeus, 1758).
abstract: This study was conducted to characterize the major proteins of the peccary seminal plasma, based on the semen samples collected from nine adult and reproductively sound animals. Our approach included the use of two-dimensional electrophoresis followed by Coomassie blue staining and analysis of polypeptide maps with PDQuest Software (Bio-Rad). Proteins were identified by tandem mass spectrometry (LC-MS/MS). We detected 179 protein spots per gel and 98 spots were identified by mass spectrometry, corresponding to 23 different proteins. The combined intensity of those spots accounted for 56.2±6% of the intensities of all spots and 60.9% of the intensities of spots presented in every protein map. Protein spots identified as clusterin represented 19.7±8.3% of the integrated optical densities of all spots detected in the seminal plasma maps. There was a negative association (r=-0.87; P<0.05) between the intensity of a clusterin spot and the percentage of sperm with functional membrane. Spermadhesin porcine seminal plasma protein 1 and bodhesin 2 comprised 5.4±1.9 and 8.8±3.9% of the total intensity of all spots respectively. Many proteins appeared in a polymorphic pattern, such as clusterin (27 spots), epididymal secretory glutathione peroxidase (ten spots), inter-α-trypsin inhibitor (12 spots), and IgG-binding protein (ten spots), among others. In conclusion, we presently describe the major seminal plasma proteome of the peccary, which exhibits a distinct high expression of clusterin isoforms. Knowledge of wild species reproductive biology is crucial for an understanding of their survival strategies and adaptation in a changing environment.
authors: Laboratory of Animal Germplasm ConservationFederal University of the Semi-arid, BR 110, Km 47, Bairro Costa e Silva, 59625-900 Mossoró, Rio Grande do Norte, BrazilDepartment of Animal ScienceFederal University of Ceará, 60021-970 Fortaleza, BrazilSchool of PharmacyUniversity of Fortaleza, 60811-905 Fortaleza, Brazil.
authors: E A A
authors: EA
authors: Santos
authors: Laboratory of Animal Germplasm ConservationFederal University of the Semi-arid, BR 110, Km 47, Bairro Costa e Silva, 59625-900 Mossoró, Rio Grande do Norte, BrazilDepartment of Animal ScienceFederal University of Ceará, 60021-970 Fortaleza, BrazilSchool of PharmacyUniversity of Fortaleza, 60811-905 Fortaleza, Brazil.
authors: P C
authors: PC
authors: Sousa
authors: Laboratory of Animal Germplasm ConservationFederal University of the Semi-arid, BR 110, Km 47, Bairro Costa e Silva, 59625-900 Mossoró, Rio Grande do Norte, BrazilDepartment of Animal ScienceFederal University of Ceará, 60021-970 Fortaleza, BrazilSchool of PharmacyUniversity of Fortaleza, 60811-905 Fortaleza, Brazil.
authors: J A M
authors: JA
authors: Martins
authors: Laboratory of Animal Germplasm ConservationFederal University of the Semi-arid, BR 110, Km 47, Bairro Costa e Silva, 59625-900 Mossoró, Rio Grande do Norte, BrazilDepartment of Animal ScienceFederal University of Ceará, 60021-970 Fortaleza, BrazilSchool of PharmacyUniversity of Fortaleza, 60811-905 Fortaleza, Brazil.
authors: R A
authors: RA
authors: Moreira
authors: Laboratory of Animal Germplasm ConservationFederal University of the Semi-arid, BR 110, Km 47, Bairro Costa e Silva, 59625-900 Mossoró, Rio Grande do Norte, BrazilDepartment of Animal ScienceFederal University of Ceará, 60021-970 Fortaleza, BrazilSchool of PharmacyUniversity of Fortaleza, 60811-905 Fortaleza, Brazil.
authors: A C O
authors: AC
authors: Monteiro-Moreira
authors: Laboratory of Animal Germplasm ConservationFederal University of the Semi-arid, BR 110, Km 47, Bairro Costa e Silva, 59625-900 Mossoró, Rio Grande do Norte, BrazilDepartment of Animal ScienceFederal University of Ceará, 60021-970 Fortaleza, BrazilSchool of PharmacyUniversity of Fortaleza, 60811-905 Fortaleza, Brazil.
authors: F B M B
authors: FB
authors: Moreno
authors: Laboratory of Animal Germplasm ConservationFederal University of the Semi-arid, BR 110, Km 47, Bairro Costa e Silva, 59625-900 Mossoró, Rio Grande do Norte, BrazilDepartment of Animal ScienceFederal University of Ceará, 60021-970 Fortaleza, BrazilSchool of PharmacyUniversity of Fortaleza, 60811-905 Fortaleza, Brazil.
authors: M F
authors: MF
authors: Oliveira
authors: Laboratory of Animal Germplasm ConservationFederal University of the Semi-arid, BR 110, Km 47, Bairro Costa e Silva, 59625-900 Mossoró, Rio Grande do Norte, BrazilDepartment of Animal ScienceFederal University of Ceará, 60021-970 Fortaleza, BrazilSchool of PharmacyUniversity of Fortaleza, 60811-905 Fortaleza, Brazil.
authors: A A
authors: AA
authors: Moura
authors: Laboratory of Animal Germplasm ConservationFederal University of the Semi-arid, BR 110, Km 47, Bairro Costa e Silva, 59625-900 Mossoró, Rio Grande do Norte, BrazilDepartment of Animal ScienceFederal University of Ceará, 60021-970 Fortaleza, BrazilSchool of PharmacyUniversity of Fortaleza, 60811-905 Fortaleza, Brazil legio2000@yahoo.com.
authors: A R
authors: AR
authors: Silva
mesh_terms: D000510:Alpha-Globulins / Q000032:analysis; D000818:Animals; D001188:Artiodactyla / Q000378:metabolism*; D002853:Chromatography, Liquid; D051152:Clusterin / Q000032:analysis; D003247:Conservation of Natural Resources; D015180:Electrophoresis, Gel, Two-Dimensional; D005979:Glutathione Peroxidase / Q000032:analysis; D008222:Lymphokines / Q000032:analysis; D008297:Male; D060066:Protein Interaction Maps; D020033:Protein Isoforms; D040901:Proteomics / Q000379:methods; D012661:Semen / Q000737:chemistry*; D029589:Seminal Plasma Proteins / Q000032:analysis*; D053719:Tandem Mass Spectrometry
chemical_list: D000510:Alpha-Globulins; D051152:Clusterin; D008222:Lymphokines; D020033:Protein Isoforms; D029589:Seminal Plasma Proteins; C013059:immunoglobulin-binding factors; C011877:inter-alpha-inhibitor; D005979:Glutathione Peroxidase