title: The effect of 5-HT and electrical field stimulation on the contractility of the whole isolated urinary bladder of Suncus murinus.
abstract: The present study used the whole isolated urinary bladder of Suncus murinus, to investigate the effect of exogenously added serotonin (5-HT) and electrical field stimulation (EFS) in the absence and presence of methysergide, a 5-HT1/2/7 receptor antagonist or the selective 5-HT7 receptor antagonist, SB269970. Further experiments investigated the involvement of potassium channel, cholinergic and purinergic systems in mediating the contractile response to EFS. Pre-treatment with methysergide reduced and increased the contractile responses to 5-HT and EFS, respectively. Pre-treatment with SB269970 increased the responses to 5-HT without modifying the EFS-induced contractions. EFS-induced contractions were not modified by pre-treatment with atropine (10μM), α-β-methylene ATP or glibenclamide. EFS-induced contractions were attenuated by cromakalim (10µM) or atropine (0.1 µM). In conclusion, the 5-HT2 receptors are likely to play a role in mediating the contractile response to 5-HT in detrusor muscle. Furthermore, EFS-induced contractions are mediated through cholinergic and an unknown neurotransmitter which is modulated by K(ATP) channels in the detrusor muscle of Suncus murinus.
authors: Division of Pharmacy and Pharmaceutical Sciences, School of Applied Sciences, University of Huddersfield, Huddersfield, Queensgates, HD1 3DH, UK. Electronic address: fajavid@hud.ac.uk.
authors: Farideh A
authors: FA
authors: Javid
authors: UROsphere S.A.S., Faculté des Sciences Pharmaceutiques, 35 Chemin des Maraichers, 31062 Toulouse Cedex 09, France. Electronic address: stefano.palea@urosphere.com.
authors: Stefano
authors: S
authors: Palea
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D019806:Cromakalim / Q000494:pharmacology; D004558:Electric Stimulation; D005260:Female; D005905:Glyburide / Q000494:pharmacology; D008297:Male; D008784:Methysergide; D009119:Muscle Contraction / Q000187:drug effects; D010636:Phenols / Q000494:pharmacology; D011985:Receptors, Serotonin / Q000502:physiology*; D012701:Serotonin / Q000494:pharmacology*; D012702:Serotonin Antagonists / Q000494:pharmacology; D012788:Shrews; D013449:Sulfonamides / Q000494:pharmacology; D001743:Urinary Bladder / Q000187:drug effects* / Q000502:physiology
chemical_list: D010636:Phenols; D011985:Receptors, Serotonin; C404922:SB 269970; D012702:Serotonin Antagonists; D013449:Sulfonamides; D019806:Cromakalim; D012701:Serotonin; D001285:Atropine; D000255:Adenosine Triphosphate; C002630:alpha,beta-methyleneadenosine 5'-triphosphate; D005905:Glyburide; D008784:Methysergide
keywords: 5-Hydroxytryptamine; Electrical field stimulation; Purinergic P2X receptors; Suncus murinus; Urinary bladder