title: Characterization of new polyol/H+ symporters in Debaryomyces hansenii.
abstract: Debaryomyces hansenii is a halotolerant yeast that produces and assimilates a wide variety of polyols. In this work we evaluate polyol transport in D. hansenii CBS 767, detecting the occurrence of polyol/H(+) (and sugar/H(+)) symporter activity, through the transient extracellular alkalinization of unbuffered starved cell suspensions. From the D. hansenii genome database, we selected nine ORFs encoding putative transporter proteins to clone in a centromeric plasmid with C-terminal GFP tagging and screened for polyol/H(+) symporters by heterologous expression in Saccharomyces cerevisiae. Five distinct D. hansenii polyol/H(+) symporters were identified and characterized, with different specificities and affinities for polyols, namely one glycerol-specific (DhStl1), one D-galactitol-specific (DhSgl1, Symporter galactitol/H(+) 1), one D-(+)-chiro-inositol-specific (DhSyi1, Symporter D-(+)-chiro-inositol/H(+) 1), one for D-sorbitol/D-mannitol/ribitol/D-arabitol/D-galactitol (DhSyl1, Symporter Polyols 1) and another for D-sorbitol/D-mannitol/ribitol/D-arabitol (DhSyl2, Symporter Polyols 2). This work contributed to the annotation of new yeast polyol transporters, including two specific for uncommon substrates as galactitol and D-(+)-chiro-inositol.
authors: Centre for Botany Applied to Agriculture (CBAA), Instituto Superior de Agronomia, University of Lisbon, Lisbon, Portugal.
authors: Iliana
authors: I
authors: Pereira
authors: Centre for Botany Applied to Agriculture (CBAA), Instituto Superior de Agronomia, University of Lisbon, Lisbon, Portugal ; Research Institute for Medicines and Pharmaceutical Sciences (iMed.UL) and Department of Biochemistry and Human Biology, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal.
authors: Ana
authors: A
authors: Madeira
authors: Centre for Botany Applied to Agriculture (CBAA), Instituto Superior de Agronomia, University of Lisbon, Lisbon, Portugal.
authors: Catarina
authors: C
authors: Prista
authors: Centre for Botany Applied to Agriculture (CBAA), Instituto Superior de Agronomia, University of Lisbon, Lisbon, Portugal.
authors: Maria C
authors: MC
authors: Loureiro-Dias
authors: Centre for Botany Applied to Agriculture (CBAA), Instituto Superior de Agronomia, University of Lisbon, Lisbon, Portugal.
authors: Maria José
authors: MJ
authors: Leandro
mesh_terms: D001692:Biological Transport / Q000502:physiology*; D055165:Debaryomyces / Q000378:metabolism*; D005690:Galactose / Q000031:analogs & derivatives / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism*; D007294:Inositol / Q000378:metabolism; D008353:Mannitol / Q000378:metabolism; D011108:Polymers / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013012:Sorbitol / Q000378:metabolism; D013402:Sugar Alcohols / Q000378:metabolism; D027981:Symporters / Q000378:metabolism*
chemical_list: D011108:Polymers; D013402:Sugar Alcohols; D027981:Symporters; C024617:polyol; C002038:galactal; D008353:Mannitol; D007294:Inositol; D013012:Sorbitol; D006859:Hydrogen; D005990:Glycerol; D005690:Galactose; C014999:arabitol