title: Disturbance of autophagy-lysosome signaling molecule expression in human gastric adenocarcinoma.
abstract: Autophagy is classified as type II programmed cell death and may participate in tumorigenesis. However, changes in autophagy-lysosome signaling and the relationship between the apoptotic cascade and gastric cancer cells have not been fully elucidated. The present study investigated the induction of autophagy in poorly differentiated human gastric adenocarcinoma. Immunoblotting revealed markedly induced autophagy in low grade differentiated gastric adenocarcinoma, indicated by elevation of microtubule-associated protein 1 light chain 3-I/II conversion and Beclin 1 in human gastric carcinomas. In addition, the diffuse (poorly differentiated) subtype showed significantly elevated Lamp2 and cathepsin B protein levels. Concomitantly, significant induction of anti-apoptotic events were indicated by changes in B-cell lymphoma 2 (Bcl-2) and X-linked inhibitor of apoptosis protein levels. Notably, confocal laser microscope data indicated co-expression of Bcl-2 and Beclin 1 in poorly differentiated human gastric adenocarcinoma. Results of this study indicate that the autophagy-lysosome signaling participates in poorly differentiated human gastric adenocarcinoma and there are intracellular links between autophagic signaling and the apoptotic cascade.
authors: Department of General Surgery, The Second Affiliated Hospital, Soochow University, Suzhou, Jiangsu 215004, P.R. China.
authors: Shao-Hua
authors: SH
authors: Wei
authors: Department of General Surgery, The Second Affiliated Hospital, Soochow University, Suzhou, Jiangsu 215004, P.R. China.
authors: Wei
authors: W
authors: Li
authors: Department of General Surgery, The Second Affiliated Hospital, Soochow University, Suzhou, Jiangsu 215004, P.R. China.
authors: Yang
authors: Y
authors: Liu
authors: Department of General Surgery, The Second Affiliated Hospital, Soochow University, Suzhou, Jiangsu 215004, P.R. China.
authors: DE-Kang
authors: DK
authors: Gao
authors: Department of General Surgery, The Second Affiliated Hospital, Soochow University, Suzhou, Jiangsu 215004, P.R. China.
authors: Jun
authors: J
authors: Pan
authors: Department of General Surgery, The Second Affiliated Hospital, Soochow University, Suzhou, Jiangsu 215004, P.R. China.
authors: Chun-Wei
authors: CW
authors: Gu
authors: Department of General Surgery, The Second Affiliated Hospital, Soochow University, Suzhou, Jiangsu 215004, P.R. China.
authors: Hao-Rong
authors: HR
authors: Wu
keywords: Beclin 1; apoptosis; autophagy; human gastric carcinomas; lysosome