title: Identification of specific cell-surface markers of adipose-derived stem cells from subcutaneous and visceral fat depots.
abstract: Adipose-derived stem/stromal cells (ASCs) from the anatomically distinct subcutaneous and visceral depots of white adipose tissue (WAT) differ in their inherent properties. However, little is known about the molecular identity and definitive markers of ASCs from these depots. In this study, ASCs from subcutaneous fat (SC-ASCs) and visceral fat (VS-ASCs) of omental region were isolated and studied. High-content image screening of over 240 cell-surface markers identified several potential depot-specific markers of ASCs. Subsequent studies revealed consistent predominant expression of CD10 in SC-ASCs and CD200 in VS-ASCs across 12 human subjects and in mice. CD10-high-expressing cells sorted from SC-ASCs differentiated better than their CD10-low-expressing counterparts, whereas CD200-low VS-ASCs differentiated better than CD200-high VS-ASCs. The expression of CD10 and CD200 is thus depot-dependent and associates with adipogenic capacities. These markers will offer a valuable tool for tracking and screening of depot-specific stem cell populations.
authors: Fat Metabolism and Stem Cell Group, Laboratory of Metabolic Medicine, Singapore Bioimaging Consortium, A STAR, Helios, 11 Biopolis Way, Singapore 138667, Singapore.
authors: Wee Kiat
authors: WK
authors: Ong
authors: Saw Swee Hock School of Public Health, National University of Singapore, MD3, 16 Medical Drive, Singapore 117597, Singapore.
authors: Chuen Seng
authors: CS
authors: Tan
authors: Fat Metabolism and Stem Cell Group, Laboratory of Metabolic Medicine, Singapore Bioimaging Consortium, A STAR, Helios, 11 Biopolis Way, Singapore 138667, Singapore.
authors: Kai Li
authors: KL
authors: Chan
authors: Fat Metabolism and Stem Cell Group, Laboratory of Metabolic Medicine, Singapore Bioimaging Consortium, A STAR, Helios, 11 Biopolis Way, Singapore 138667, Singapore.
authors: Grace Gandi
authors: GG
authors: Goesantoso
authors: Fat Metabolism and Stem Cell Group, Laboratory of Metabolic Medicine, Singapore Bioimaging Consortium, A STAR, Helios, 11 Biopolis Way, Singapore 138667, Singapore.
authors: Xin Hui Derryn
authors: XH
authors: Chan
authors: Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, MD6, 14 Medical Drive, Singapore 117599, Singapore.
authors: Edmund
authors: E
authors: Chan
authors: Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, MD6, 14 Medical Drive, Singapore 117599, Singapore.
authors: Jocelyn
authors: J
authors: Yin
authors: Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, MD6, 14 Medical Drive, Singapore 117599, Singapore.
authors: Chia Rou
authors: CR
authors: Yeo
authors: Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, MD6, 14 Medical Drive, Singapore 117599, Singapore.
authors: Chin Meng
authors: CM
authors: Khoo
authors: Department of Surgery, National University Hospital, 5 Lower Kent Ridge Road, Singapore 119074, Singapore.
authors: Jimmy Bok Yan
authors: JB
authors: So
authors: Department of Surgery, National University Hospital, 5 Lower Kent Ridge Road, Singapore 119074, Singapore.
authors: Asim
authors: A
authors: Shabbir
authors: Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, MD6, 14 Medical Drive, Singapore 117599, Singapore.
authors: Sue-Anne
authors: SA
authors: Toh
authors: Cardiovascular and Metabolic Disorders Program, Duke-NUS Graduate Medical School, 8 College Road, Singapore 169857, Singapore ; Laboratory of Metabolic Medicine, Singapore Bioimaging Consortium and Metabolism in Human Diseases Unit, Institute of Molecular and Cell Biology, A STAR, Singapore 138667, Singapore.
authors: Weiping
authors: W
authors: Han
authors: Fat Metabolism and Stem Cell Group, Laboratory of Metabolic Medicine, Singapore Bioimaging Consortium, A STAR, Helios, 11 Biopolis Way, Singapore 138667, Singapore ; Cardiovascular and Metabolic Disorders Program, Duke-NUS Graduate Medical School, 8 College Road, Singapore 169857, Singapore.
authors: Shigeki
authors: S
authors: Sugii
mesh_terms: D050156:Adipogenesis; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D015870:Gene Expression; D057166:High-Throughput Screening Assays; D006801:Humans; D016130:Immunophenotyping; D050152:Intra-Abdominal Fat / Q000166:cytology*; D008297:Male; D051379:Mice; D015260:Neprilysin / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*; D050151:Subcutaneous Fat / Q000166:cytology*
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; D015415:Biomarkers; D015260:Neprilysin; C042739:antigens, CD200