title: Chemical characterization and complement fixation of pectins from Cola cordifolia leaves.
abstract: Defatted leaves from the medicinal tree Cola cordifolia were extracted with 50% EtOH, 50 °C and 100 °C water. The polysaccharide rich extracts were fractionated and the structure of the polysaccharides elucidated. Linkage analysis of the polysaccharides indicates a rhamnogalacturonan type I backbone where both Rha and parts of GalA are substituted in position 3, indicating a highly branched polymer with short side chains. The purified fractions were tested for complement fixation, macrophage stimulating activity and anti-adhesion activity towards Helicobacter pylori. Here we report on complex and polydisperse types of pectins (Mw: 3-1300 kDa) as well as the presence of low Mw (<3 kDa) acidic oligosaccharides. The fractions showed a moderate complement fixing activity and no macrophage activating effects after LPS removal. Anti-adhesion activity towards H. pylori was not found.
authors: University of Oslo, School of Pharmacy, 1068 Blindern, 0316 Oslo, Norway. Electronic address: ingvild.austarheim@farmasi.uio.no.
authors: Ingvild
authors: I
authors: Austarheim
authors: NTNU, 7491 Trondheim, Norway.
authors: Bjørn E
authors: BE
authors: Christensen
authors: University of Oslo, School of Pharmacy, 1068 Blindern, 0316 Oslo, Norway.
authors: Hoai Thi Nguyen
authors: HT
authors: Aas
authors: Institute of Pharmaceutical Biology and Phytochemistry, Corrensstr. 48, 48149 Münster, Germany.
authors: Christian
authors: C
authors: Thöle
authors: University of Oslo, School of Pharmacy, 1068 Blindern, 0316 Oslo, Norway; Department of Traditional Medicine, P. B. 1746 Bamako, Mali.
authors: Drissa
authors: D
authors: Diallo
authors: University of Oslo, School of Pharmacy, 1068 Blindern, 0316 Oslo, Norway.
authors: Berit S
authors: BS
authors: Paulsen
mesh_terms: D000818:Animals; D002460:Cell Line; D030221:Cola / Q000737:chemistry*; D003165:Complement System Proteins / Q000737:chemistry / Q000187:drug effects*; D016480:Helicobacter pylori / Q000187:drug effects; D051379:Mice; D010368:Pectins / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: C042491:rhamnogalacturonan I; D010368:Pectins; D003165:Complement System Proteins
keywords: Cola cordifolia; Complement fixation; Helicobacter pylori; LPS; Pectin; Traditional medicine