title: Identification of anti-allergic effect of Clonorchis sinensis-derived protein venom allergen-like proteins (CsVAL).
abstract: Previous studies demonstrated that Clonochis sinensis-derived crude antigens suppress development of allergic responses. We investigated the effects of C. sinensis venom allergen-like (CsVAL) proteins on immune-modulating activities in allergic inflammatory response. Using RBL-2H3 rat mast cells, we demonstrated that CsVAL inhibits antigen-induced β-hexosaminidase release from immunoglobulin E-sensitized RBL-2H3 cells, and this inhibitory activity occurs by suppressing Lyn phosphorylation and intracellular reactive oxygen species production. In addition, CsVAL peptide treatment inhibits activation of protein kinase C-α and extracellular signal-regulated kinase 1/2, which are involved in degranulation of immunoglobulin E-sensitized mast cells. Furthermore, immunization with CsVAL suppressed development of skin inflammation by assessing ear thickness and cutaneous infiltration by eosinophils and mast cells in oxazolone-induced contact hypersensitivity in vivo mouse model. These results suggest that CsVAL is a promising candidate as an effective mast cell inhibitor for allergic and inflammatory diseases.
authors: Division of Malaria & Parasitic Disease, Korea National Institute of Health, 187 Osong Saengmyeong2-ro, Cheongwon-gun, Chungbuk 361-951, Republic of Korea.
authors: Young-Il
authors: YI
authors: Jeong
authors: Division of Malaria & Parasitic Disease, Korea National Institute of Health, 187 Osong Saengmyeong2-ro, Cheongwon-gun, Chungbuk 361-951, Republic of Korea.
authors: Yu-Jung
authors: YJ
authors: Kim
authors: Division of Malaria & Parasitic Disease, Korea National Institute of Health, 187 Osong Saengmyeong2-ro, Cheongwon-gun, Chungbuk 361-951, Republic of Korea.
authors: Jung-Won
authors: JW
authors: Ju
authors: Division of Malaria & Parasitic Disease, Korea National Institute of Health, 187 Osong Saengmyeong2-ro, Cheongwon-gun, Chungbuk 361-951, Republic of Korea.
authors: Sung-Hee
authors: SH
authors: Hong
authors: Division of Malaria & Parasitic Disease, Korea National Institute of Health, 187 Osong Saengmyeong2-ro, Cheongwon-gun, Chungbuk 361-951, Republic of Korea.
authors: Myoung-Ro
authors: MR
authors: Lee
authors: Division of Malaria & Parasitic Disease, Korea National Institute of Health, 187 Osong Saengmyeong2-ro, Cheongwon-gun, Chungbuk 361-951, Republic of Korea.
authors: Shin-Hyeong
authors: SH
authors: Cho
authors: Division of Malaria & Parasitic Disease, Korea National Institute of Health, 187 Osong Saengmyeong2-ro, Cheongwon-gun, Chungbuk 361-951, Republic of Korea.
authors: Won-Ja
authors: WJ
authors: Lee
authors: Division of Malaria & Parasitic Disease, Korea National Institute of Health, 187 Osong Saengmyeong2-ro, Cheongwon-gun, Chungbuk 361-951, Republic of Korea. Electronic address: ondalgl@korea.kr.
authors: Sang-Eun
authors: SE
authors: Lee
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000276:immunology* / Q000627:therapeutic use; D000947:Antigens, Helminth / Q000276:immunology* / Q000627:therapeutic use; D015550:Cell Degranulation / Q000187:drug effects; D002460:Cell Line; D003004:Clonorchis sinensis / Q000276:immunology*; D003877:Dermatitis, Contact / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D005260:Female; D015801:Helminth Proteins / Q000276:immunology* / Q000627:therapeutic use; D007073:Immunoglobulin E / Q000276:immunology; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology; D008807:Mice, Inbred BALB C; D051381:Rats; D012867:Skin / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D001619:beta-N-Acetylhexosaminidases / Q000276:immunology
chemical_list: D018926:Anti-Allergic Agents; D000947:Antigens, Helminth; D015801:Helminth Proteins; D007073:Immunoglobulin E; D001619:beta-N-Acetylhexosaminidases
keywords: Clonochis sinensis; Clonochis sinensis venom allergen-like (CsVAL) proteins; Contact hypersensitivity; RBL-2H3 rat mast cell