title: Human Bone Marrow Mesenchymal Stem Cell Behaviors on PCL/Gelatin Nanofibrous Scaffolds Modified with A Collagen IV-Derived RGD-Containing Peptide.
abstract: OBJECTIVE
  We introduce an RGD (Arg-Gly-Asp)-containing peptide of collagen IV origin that possesses potent cell adhesion and proliferation properties.
  
  
  METHODS
  In this experimental study, the peptide was immobilized on an electrospun nanofibrous polycaprolactone/gelatin (PCL/Gel) hybrid scaffold by a chemical bonding approach to improve cell adhesion properties of the scaffold. An iodine-modified phenylalanine was introduced in the peptide to track the immobilization process. Native and modified scaffolds were characterized with scanning electronmicroscopy (SEM) and fourier transform infrared spectroscopy (FTIR). We studied the osteogenic and adipogenic differentiation potential of human bone marrow-derived mesenchymal stem cells (hBMSCs). In addition, cell adhesion and proliferation behaviors of hBMSCs on native and peptide modified scaffolds were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and 4',6-diamidino-2-phenylindole (DAPI) staining, and the results compared with tissue culture plate, as the control.
  
  
  RESULTS
  FTIR results showed that the peptide successfully immobilized on the scaffold.MTT assay and DAPI staining results indicated that peptide immobilization had a dramatic effect on cell adhesion and proliferation.
  
  
  CONCLUSIONS
  This peptide modified nanofibrous scaffold can be a promising biomaterial for tissue engineering and regenerative medicine with the use of hBMSCs.
authors: Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors: Ali
authors: A
authors: Mota
authors: Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors: Abbas
authors: A
authors: Sahebghadam Lotfi
authors: Iran Polymer and Petrochemical Institute (IPPI), Tehran, Iran.
authors: Jalal
authors: J
authors: Barzin
authors: National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
authors: Mostafa
authors: M
authors: Hatam
authors: Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors: Behzad
authors: B
authors: Adibi
authors: National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
authors: Zahra
authors: Z
authors: Khalaj
authors: National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
authors: Mohammad
authors: M
authors: Massumi
keywords: Baicalin; Erythropoiesis; Hematopoietic Stem Cell; PPARgamma