title: Misregulation of the broad-range phospholipase C activity increases the susceptibility of Listeria monocytogenes to intracellular killing by neutrophils.
abstract: Listeria monocytogenes is a facultative intracellular bacterial pathogen that tightly regulates the activities of various virulence factors during infection. A mutant strain (the plcBDpro mutant) that has lost the ability to control the activity of a phospholipase C (PC-PLC) is attenuated a hundred fold in mice. This attenuation is not due to a lack of bacterial fitness, but appears to result from a modified host response to infection. The transcriptomic pattern of immune-related genes indicated that PC-PLC did not enhance the innate immune response in infected macrophages. However, it partially protected the cells from bacteria-mediated mitochondrial fragmentation. In mice, the plcBDpro mutant transiently caused an increase in liver pathology, as judged by the size of neutrophil-filled micro-abscesses. Moreover, the plcBDpro mutant was more susceptible to intracellular killing by neutrophils than wild-type L. monocytogenes. Together, these data indicate that in vivo attenuation of the plcBDpro mutant results from its reduced ability to disrupt mitochondrial homeostasis and to resist intracellular killing by neutrophils.
authors: Bryant S
authors: BS
authors: Blank
authors: Delbert S
authors: DS
authors: Abi Abdallah
authors: Justin J
authors: JJ
authors: Park
authors: Evgeniya V
authors: EV
authors: Nazarova
authors: Alan
authors: A
authors: Pavinski Bitar
authors: Kirk J
authors: KJ
authors: Maurer
authors: Hélène
authors: H
authors: Marquis
mesh_terms: D000818:Animals; D005260:Female; D008089:Listeria monocytogenes / Q000201:enzymology* / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D050296:Microbial Viability; D009504:Neutrophils / Q000276:immunology* / Q000382:microbiology*; D010738:Type C Phospholipases / Q000235:genetics / Q000378:metabolism*; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D037521:Virulence Factors; D010738:Type C Phospholipases