title: Replication of prions in differentiated muscle cells.
abstract: We have demonstrated that prions accumulate to high levels in non-proliferative C2C12 myotubes. C2C12 cells replicate as myoblasts but can be differentiated into myotubes. Earlier studies indicated that C2C12 myoblasts are not competent for prion replication. (1) We confirmed that observation and demonstrated, for the first time, that while replicative myoblasts do not accumulate PrP(Sc), differentiated post-mitotic myotube cultures replicate prions robustly. Here we extend our observations and describe the implication and utility of this system for replicating prions.
authors: Centre for Prions and Protein Folding Diseases; Department of Agricultural, Food and Nutritional Sciences; University of Alberta; Edmonton, AB Canada.
authors: Allen
authors: A
authors: Herbst
authors: Centre for Prions and Protein Folding Diseases; Department of Agricultural, Food and Nutritional Sciences; University of Alberta; Edmonton, AB Canada.
authors: Judd M
authors: JM
authors: Aiken
authors: Centre for Prions and Protein Folding Diseases; Department of Biological Sciences; University of Alberta; Edmonton, AB Canada.
authors: Debbie
authors: D
authors: McKenzie
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D051379:Mice; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism*; D011328:Prions / Q000096:biosynthesis*
chemical_list: D011328:Prions
keywords: C2C12; Differentiation; PrP; Prnp; Scrapie; muscle; myotube; prion; prion replication