title: Highly stable colorimetric aptamer sensors for detection of ochratoxin A through optimizing the sequence with the covalent conjugation of hemin.
abstract: Optimization of hairpin DNA is introduced to detect ochratoxin A (OTA) by chemically conjugating its cofactor, hemin, toward the 5'-end. The newly designed OTA aptasensor showed enhanced stability and sensitivity, thereby lowering the detection limit to an ~1 nM level. Furthermore, an optimal spacer for hemin conjugation was investigated for stable responses toward very diluted OTA solutions.
authors: Nanobiotechnology (Major), School of Engineering, University of Science & Technology Centre, Daejeon 305-806, Republic of Korea. taihwan@kribb.re.kr.
authors: Jayeon
authors: J
authors: Lee
authors: Chang Hoon
authors: CH
authors: Jeon
authors: Sang Jung
authors: SJ
authors: Ahn
authors: Tai Hwan
authors: TH
authors: Ha
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D003124:Colorimetry / Q000295:instrumentation / Q000379:methods*; D006427:Hemin / Q000737:chemistry*; D057230:Limit of Detection; D008969:Molecular Sequence Data; D009793:Ochratoxins / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D009793:Ochratoxins; C025589:ochratoxin A; D006427:Hemin