title: Panduratin A, an activator of PPAR-α/δ, suppresses the development of oxazolone-induced atopic dermatitis-like symptoms in hairless mice.
abstract: OBJECTIVE
  Panduratin A isolated from Boesenbergia pandurata (Roxb.) has been reported to have antioxidant, anti-inflammatory, and anti-allergic activities. However, the effect of panduratin A on atopic dermatitis (AD) has not been studied. In the present study, we investigated the efficacy of panduratin A, an activator of peroxisome proliferator-activated receptors (PPAR) α/δ, using oxazolone-induced AD-like model in hairless mice.
  
  
  METHODS
  To determine PPARα/δ activation of panduratin A, HaCaT, Hs68, and COS-7 cells were treated with panduratin A, then PPARα/δ and PPAR response element (PPRE) activities were assessed with a reporter gene assay. For the in vivo study, oral administration of panduratin A was performed for 4weeks, with oxazolone treatment every other day. The efficacy of panduratin A on parameters of oxazolone-induced AD was assessed physiologically, morphologically, and immunologically.
  
  
  RESULTS
  Panduratin A increased PPARα/δ and PPRE activation both in vitro and in vivo. Panduratin A attenuated dermatitis-associated barrier damage as demonstrated by transepidermal water loss, erythema, and filaggrin expression. Furthermore, infiltration of inflammatory cells and epidermal thickness in the skin were decreased. Panduratin A decreased serum immunoglobulin (Ig) E and interleukin-4 levels but increased IgG2a and interferon-γ levels. In addition, panduratin A decreased inflammation-associated molecules in the skin. Panduratin A also decreased Th2-associated molecules and increased Th1/regulatory T cell (Treg)-associated molecules in the spleen.
  
  
  CONCLUSIONS
  Panduratin A showed a beneficial effect on AD by modulating Th1/Th2/Treg-associated immune response and is a potential candidate for treating AD.
authors: Department of Biotechnology, Yonsei University, Seoul, South Korea.
authors: Myung-Suk
authors: MS
authors: Kim
authors: Department of Biomaterials Science and Engineering, Yonsei University, Seoul, South Korea.
authors: Hee-Bong
authors: HB
authors: Pyun
authors: Department of Biotechnology, Yonsei University, Seoul, South Korea; Department of Biomaterials Science and Engineering, Yonsei University, Seoul, South Korea. Electronic address: jkhwang@yonsei.ac.kr.
authors: Jae-Kwan
authors: JK
authors: Hwang
mesh_terms: D000818:Animals; D019556:COS Cells; D047188:Chalcones / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D016207:Cytokines / Q000097:blood / Q000235:genetics; D003876:Dermatitis, Atopic / Q000097:blood / Q000139:chemically induced / Q000276:immunology / Q000517:prevention & control*; D004353:Drug Evaluation, Preclinical; D005260:Female; D000091344:Filaggrin Proteins; D017930:Genes, Reporter; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008812:Mice, Hairless; D010081:Oxazolone; D047493:PPAR alpha / Q000378:metabolism; D047494:PPAR delta / Q000378:metabolism; D012867:Skin / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D015533:Transcriptional Activation / Q000187:drug effects
chemical_list: D047188:Chalcones; D016207:Cytokines; C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D007074:Immunoglobulin G; D047493:PPAR alpha; D047494:PPAR delta; D010081:Oxazolone; C482884:panduratin A; D007073:Immunoglobulin E
keywords: Atopic dermatitis; Boesenbergia pandurata (Roxb.) Schltr; Oxazolone; Panduratin A; Transepidermal water loss