title: Differentiation of human induced pluripotent stem cells into a keratinocyte lineage.
abstract: Direct reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) provides an opportunity to develop novel personalized treatment options for numerous diseases and to advance current approaches for cell-based drug discoveries and disease modeling. The ability to differentiate iPSCs into relevant cell types is an important prerequisite for the successful development of iPSC-based treatment and modeling strategies. Here, we describe a protocol for the efficient differentiation of human iPSCs into functional keratinocytes. The protocol employs treating iPSCs with retinoic acid and bone-morphogenetic protein-4 to induce differentiation toward a keratinocyte lineage, which is then followed by the growth of differentiated iPSCs on collagen type I- and collagen type IV-coated dishes to enrich for iPSC-derived keratinocytes.
authors: Charles C. Gates Center for Regenerative Medicine and Stem Cell Biology, University of Colorado, Anschutz Medical Campus, Mail Stop 8320, 6511, Aurora, CO, 80045, USA.
authors: Igor
authors: I
authors: Kogut
authors: Dennis R
authors: DR
authors: Roop
authors: Ganna
authors: G
authors: Bilousova
mesh_terms: D055415:Bone Morphogenetic Protein 4 / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects; D024042:Collagen Type I / Q000494:pharmacology; D024141:Collagen Type IV / Q000494:pharmacology; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects; D015603:Keratinocytes / Q000166:cytology*; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D055415:Bone Morphogenetic Protein 4; D024042:Collagen Type I; D024141:Collagen Type IV; D014212:Tretinoin