title: Detection of carbapenemases by real-time PCR and melt curve analysis on the BD Max system.
abstract: We developed a multiplex SYBR green real-time PCR for the BD Max instrument (BD Diagnostics, Sparks, MD) to detect a panel of carbapenemases. The assay was evaluated with 152 consecutive isolates sent to the German National Reference Laboratory, and 65/65 of the carbapenemase-positive and 87/87 of the carbapenemase-negative strains were identified correctly.
authors: Department of Infectious Diseases, Medical Microbiology and Hygiene, Heidelberg University Hospital, Heidelberg, Germany.
authors: Marjeta
authors: M
authors: Hofko
authors: Alexander
authors: A
authors: Mischnik
authors: Martin
authors: M
authors: Kaase
authors: Stefan
authors: S
authors: Zimmermann
authors: Alexander H
authors: AH
authors: Dalpke
mesh_terms: D001419:Bacteria / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase