title: Polychlorinated biphenyl quinone-induced genotoxicity, oxidative DNA damage and γ-H2AX formation in HepG2 cells.
abstract: Our previous study has demonstrated that PCB quinone is cytotoxic in HepG2 cells (Toxicology in Vitro 26 (2012) 841-848). However, it is not clear whether PCB quinone is also carcinogenic (or mutagenic). In the current study, we investigated the genotoxicity of PCB quinone (2,3,5-trichloro-6-phenyl-[1,4]benzoquinone, PCB29-pQ) in HepG2 cells using single cell gel electrophoresis (SCGE) assay and micronucleus (MN) assay. We found PCB29-pQ exposure significantly increased olive tail moment (OTM) and micronuclei (MN) frequencies in HepG2 cells. These data suggested that PCB29-pQ caused DNA strand breaks and chromosome breaks. We further investigated whether the genotoxicity of PCB29-pQ is associated with the generation of reactive oxygen species (ROS). Using enzyme-linked immunosorbent assay for 8-hydroxydeoxyguanosine (8-OHdG) detection, we demonstrated that the level of oxidative DNA damage was significantly evaluated with PCB29-pQ exposure concentration and time dependently. Moreover, γ-H2AX appeared after the treatment of PCB29-pQ in HepG2 cells, may indicate double strand breaks (DSBs). In addition, the pretreatment of ROS scavengers inhibited the genotoxicity of PCB29-pQ significantly. In conclusion, our data suggested that PCB29-pQ causes genotoxic effects in HepG2 cells, probably via ROS-induced oxidative DNA damage.
authors: Key Laboratory of Luminescence and Real-Time Analysis, Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, PR China.
authors: Hui
authors: H
authors: Dong
authors: Key Laboratory of Luminescence and Real-Time Analysis, Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, PR China.
authors: Chuanyang
authors: C
authors: Su
authors: Key Laboratory of Luminescence and Real-Time Analysis, Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, PR China.
authors: Xiaomin
authors: X
authors: Xia
authors: Key Laboratory of Luminescence and Real-Time Analysis, Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, PR China.
authors: Lingrui
authors: L
authors: Li
authors: Key Laboratory of Luminescence and Real-Time Analysis, Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, PR China.
authors: Erqun
authors: E
authors: Song
authors: Key Laboratory of Luminescence and Real-Time Analysis, Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, PR China. Electronic address: ysong@swu.edu.cn.
authors: Yang
authors: Y
authors: Song
mesh_terms: D016227:Benzoquinones / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D004249:DNA Damage; D023381:Endpoint Determination; D016166:Free Radical Scavengers / Q000494:pharmacology; D056945:Hep G2 Cells; D006657:Histones / Q000096:biosynthesis* / Q000378:metabolism; D006801:Humans; D009153:Mutagens / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects* / Q000235:genetics*; D010766:Phosphorylation / Q000187:drug effects; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D016227:Benzoquinones; D016166:Free Radical Scavengers; C468783:H2AX protein, human; D006657:Histones; D009153:Mutagens; D017382:Reactive Oxygen Species; C004532:quinone; D011078:Polychlorinated Biphenyls
keywords: Genotoxicity; Oxidative stress; PCB; Quinone; ROS