title: First isolation and molecular characterization of Toxoplasma gondii from a human placenta in Argentina.
abstract: Blood sample and placenta were taken from a 37-week pregnant woman; serologic results indicated acute toxoplasmosis. Placenta was inoculated into mice. Seropositive mice were sacrificed and tissue cysts from brain were inoculated into new mice. Specific DNA was detected by PCR, and the isolate was characterized as Type II by nPCR-RFLP for nSAG2, SAG3, BTUB, GRA6, c29-2, c22-8, L358, PK1 and Apico markers. This is the first isolation and molecular characterization of Toxoplasma gondii from humans in Argentina.
authors: Laboratorio de Inmunoparasitología, FCV, UNLP, Argentina; CONICET, Argentina. Electronic address: laispardini@fcv.unlp.edu.ar.
authors: Lais
authors: L
authors: Pardini
authors: Centro de toxoplasmosis y otras zoonosis, Hospital Alemán, Buenos Aires, Argentina.
authors: Liliana A
authors: LA
authors: Carral
authors: Laboratorio de Inmunoparasitología, FCV, UNLP, Argentina.
authors: Mariana
authors: M
authors: Bernstein
authors: Laboratorio de Inmunoparasitología, FCV, UNLP, Argentina; CONICET, Argentina.
authors: María L
authors: ML
authors: Gos
authors: Centro de toxoplasmosis y otras zoonosis, Hospital Alemán, Buenos Aires, Argentina.
authors: Patricia
authors: P
authors: Olejnik
authors: Laboratorio de Inmunoparasitología, FCV, UNLP, Argentina.
authors: Juan M
authors: JM
authors: Unzaga
authors: Centro de toxoplasmosis y otras zoonosis, Hospital Alemán, Buenos Aires, Argentina.
authors: Federico J
authors: FJ
authors: Kaufer
authors: Centro de toxoplasmosis y otras zoonosis, Hospital Alemán, Buenos Aires, Argentina.
authors: Cristina B
authors: CB
authors: Freuler
authors: Centro de toxoplasmosis y otras zoonosis, Hospital Alemán, Buenos Aires, Argentina.
authors: Ricardo A
authors: RA
authors: Durlach
authors: Laboratorio de Inmunoparasitología, FCV, UNLP, Argentina.
authors: María C
authors: MC
authors: Venturini
mesh_terms: D000818:Animals; D001118:Argentina / Q000453:epidemiology; D001681:Biological Assay; D003049:Coccidiostats / Q000627:therapeutic use; D005260:Female; D006801:Humans; D018445:Infectious Disease Transmission, Vertical; D051379:Mice; D010920:Placenta / Q000469:parasitology*; D011247:Pregnancy; D015572:Spiramycin / Q000627:therapeutic use; D014122:Toxoplasma / Q000302:isolation & purification*; D014123:Toxoplasmosis / Q000188:drug therapy / Q000453:epidemiology* / Q000635:transmission*
chemical_list: D003049:Coccidiostats; D015572:Spiramycin
keywords: Genotyping; Human; Isolation; Placenta; Toxoplasma gondii