title: Case study 1. Practical considerations with experimental design and interpretation.
abstract: At some point, anyone with knowledge of drug metabolism and enzyme kinetics started out knowing little about these topics. This chapter was specifically written with the novice in mind. Regardless of the enzyme one is working with or the goal of the experiment itself, there are fundamental components and concepts of every experiment using drug metabolism enzymes. The following case studies provide practical tips, techniques, and answers to questions that may arise in the course of conducting such experiments. Issues ranging from assay design and development to data interpretation are addressed. The goal of this section is to act as a starting point to provide the reader with key questions and guidance while attempting his/her own work.
authors: Department of Chemistry, Washington State University, Pullman, WA, USA.
authors: John T
authors: JT
authors: Barr
authors: Darcy R
authors: DR
authors: Flora
authors: Otito F
authors: OF
authors: Iwuchukwu
mesh_terms: D003627:Data Interpretation, Statistical; D057075:Enzyme Assays / Q000379:methods* / Q000592:standards; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004798:Enzymes / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D016014:Linear Models; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D012015:Reference Standards; D012107:Research Design; D012997:Solvents / Q000737:chemistry
chemical_list: D004791:Enzyme Inhibitors; D004798:Enzymes; D004364:Pharmaceutical Preparations; D012997:Solvents