title: Protease-activated receptors expression in gingiva in periodontal health and disease.
abstract: OBJECTIVE
  Protease-activated receptors (PARs) are a unique class of receptors which are implicated in mediating inflammation, pain and other functions. The aim of this study was to elucidate the role of PARs in the pathogenesis of chronic periodontitis by differential expression analysis of PARs in the gingival tissues of chronic periodontitis patients compared with those of healthy control individuals.
  
  
  METHODS
  Gingival tissue specimens were collected from chronic periodontitis patients (n=20) and control individuals (n=20). The expression of PAR-1, -2, -3 and -4 was determined in these tissues by immunohistochemistry and differential expression between the two groups was investigated by quantitative real-time reverse transcription-polymerase chain reaction analysis.
  
  
  RESULTS
  PAR-1, -2, -3 and -4 were expressed in all gingival tissues. A significant overexpression of PAR-3 was detected in chronic periodontitis-affected tissues compared to healthy gingival tissues. However, expression of PAR-2 was decreased in periodontal lesions.
  
  
  CONCLUSIONS
  Our study shows that PAR-1, -2, -3 and -4 are expressed in both healthy and inflamed gingival tissues. Furthermore, PAR-2 and PAR-3 may contribute to the inflammatory responses associated with chronic periodontitis.
authors: Dental Department, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China.
authors: Diya
authors: D
authors: Zhang
authors: Department of Oral and Maxillofacial Surgery, Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.
authors: Shenglai
authors: S
authors: Li
authors: Department of Oral Medicine, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.
authors: Lingjing
authors: L
authors: Hu
authors: Dental Department, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China.
authors: Lieping
authors: L
authors: Sheng
authors: Dental Department, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China.
authors: Zheng
authors: Z
authors: Cao
authors: Department of Oral Medicine, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.
authors: Yanmin
authors: Y
authors: Wu
authors: Department of Oral Medicine, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China. Electronic address: chenlili_1030@163.com.
authors: Lili
authors: L
authors: Chen
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000378:metabolism*; D005260:Female; D005881:Gingiva / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D044462:Receptors, Proteinase-Activated / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D044462:Receptors, Proteinase-Activated
keywords: Chronic periodontitis; Gingiva; Immunohistochemistry; Protease-activated receptor; Reverse transcriptase polymerase chain reaction