title: In vitro and in vivo antioxidant activity of a fructan from the roots of Arctium lappa L.
abstract: To explore new antioxidant resource from food, a water-soluble polysaccharide (ALP1) was extracted and purified from the roots of Arctium lappa L. (A. lappa L.) through hot water extraction followed by ethanol precipitation, ion-exchange chromatography and gel filtration. The antioxidant activity of ALP1 was then evaluated in vitro and in vivo. ALP1 was characterized as a fructan composed of fructose and glucose in the ratio of 13.0:1.0, with an average molecular weight of 4600 Da. The linkages in ALP1 were →1)-Fruf-(2→, Fruf-(2→ and Glcp-(1→. In vitro antioxidant assays demonstrated that ALP1 possessed moderate ABTS(+) scavenging activity, strong hydroxyl radical scavenging activity and strong ferrous ion chelating activity. In in vivo antioxidant assays, ALP1 administration significantly enhanced antioxidant enzyme activities and total antioxidant capacity, as well as decreased the levels of malondialdehyde (MDA) in both the serum and liver of aging mice. These results suggest that ALP1 has potential as a novel natural antioxidant in food industry and pharmaceuticals.
authors: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China; School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, PR China.
authors: Wei
authors: W
authors: Liu
authors: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China; School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, PR China.
authors: Jiajia
authors: J
authors: Wang
authors: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China; School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, PR China.
authors: Zhenzhen
authors: Z
authors: Zhang
authors: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China; School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, PR China.
authors: Jinnan
authors: J
authors: Xu
authors: International Chemistry Testing, Milford, MA 01757, USA.
authors: Zhuohong
authors: Z
authors: Xie
authors: Department of Nutrition and Food Studies, George Mason University, Fairfax, VA 22030, USA.
authors: Margaret
authors: M
authors: Slavin
authors: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China; School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, PR China. Electronic address: xdgao@cpu.edu.cn.
authors: Xiangdong
authors: X
authors: Gao
mesh_terms: D000818:Animals; D031170:Arctium / Q000737:chemistry*; D055598:Chemical Phenomena; D005260:Female; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology*; D005609:Free Radicals / Q000737:chemistry; D005630:Fructans / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D018517:Plant Roots / Q000737:chemistry*
chemical_list: D016166:Free Radical Scavengers; D005609:Free Radicals; D005630:Fructans
keywords: Antioxidant activity; Arctium lappa L.; Fructan