title: Influences of protein ingestion on glucagon-like peptide (GLP)-1-immunoreactive endocrine cells in the chicken ileum.
abstract: Influences of a specific dietary nutrient on glucagon-like peptide (GLP)-1-containing cells in the chicken intestine are not yet clear. Significance of dietary protein level on GLP-1-containing cells in the chicken ileum was investigated. Chickens fed control or experimental diets of varying protein levels were examined using immunohistochemical and morphometrical techniques. We show that the protein ingestion had an impact on the activities of GLP-1-immunoreactive cells in the chicken ileum. Weight gains declined with decreasing dietary crude protein (CP) levels, but no significant differences were detected in the daily feed intake and villous height. GLP-1-immunoreactive cells with a round or oval shape were frequently observed in the lower CP level groups (4.5% and 0%). Frequencies of occurrence of GLP-1-immunoreactive cells were 41.1 ± 4.1, 38.5 ± 4, 34.8 ± 3.1 and 34.3 ± 3.7 (cells/mm(2) , mean ± SD) for dietary CP level of 18%, 9%, 4.5% and 0% groups, respectively and significant differences were recognized between the control and lower CP level groups (P<0.05). Multiple regression analysis indicated a significant correlation between the daily protein intake and frequencies of occurrence of GLP-1-immunoreactive cells. The protein ingestion is one of the signals that influence GLP-1-containing cells in the chicken small intestine.
authors: Department of Bioscience and Food Production Science, Interdisciplinary Graduate School of Science and Technology, Kami-ina, Japan.
authors: Mohammad M
authors: MM
authors: Monir
authors: Kohzy
authors: K
authors: Hiramatsu
authors: Sachiko
authors: S
authors: Matsumoto
authors: Kei
authors: K
authors: Nishimura
authors: Chihiro
authors: C
authors: Takemoto
authors: Tomomi
authors: T
authors: Shioji
authors: Takafumi
authors: T
authors: Watanabe
authors: Kazumi
authors: K
authors: Kita
authors: Shinichi
authors: S
authors: Yonekura
authors: Sang Gun
authors: SG
authors: Roh
mesh_terms: D000818:Animals; D002645:Chickens / Q000033:anatomy & histology* / Q000276:immunology; D004044:Dietary Proteins / Q000494:pharmacology*; D019858:Enteroendocrine Cells / Q000166:cytology* / Q000276:immunology; D052216:Glucagon-Like Peptide 1; D007082:Ileum / Q000166:cytology* / Q000276:immunology; D007150:Immunohistochemistry; D008297:Male
chemical_list: D004044:Dietary Proteins; D052216:Glucagon-Like Peptide 1
keywords: chicken; glucagon-like peptide-1; immunohistochemistry; intestine; protein ingestion