title: HPLC-fluorescence determination of thiol compounds in the serum of human male and female subjects using HILIC-mode column.
abstract: High-performance liquid chromatography-fluorescence detection using a hydrophilic interaction chromatography-mode column (ZIC®-HILIC) was used to determine four kinds of thiol compounds in human serum. Sera were obtained from 34 subjects for this study (17 male subjects aged 22-38 years and 17 female subjects aged 18-38 years). Serum cysteine, cysteinylglycine, glutathione, and γ-glutamylcysteine, derivatized with ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate, were separated on the ZIC®-HILIC column and quantified. The serum concentrations of cysteine, cysteinylglycine, glutathione and γ-glutamylcysteine were 226 ± 4.7, 23.4 ± 1.3, 3.7 ± 0.2 and 3.2 ± 0.1 μm, respectively. In addition, the concentrations of serum thiol compounds from male subjects were significantly higher than those of the female subjects (p < 0.05).
authors: Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi-shi, Chiba, 274-8510, Japan.
authors: Yumiko
authors: Y
authors: Kono
authors: Hideaki
authors: H
authors: Iizuka
authors: Muneki
authors: M
authors: Isokawa
authors: Makoto
authors: M
authors: Tsunoda
authors: Hideaki
authors: H
authors: Ichiba
authors: Kiyomi
authors: K
authors: Sadamoto
authors: Takeshi
authors: T
authors: Fukushima
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D003545:Cysteine / Q000031:analogs & derivatives / Q000097:blood*; D004151:Dipeptides; D005260:Female; D005453:Fluorescence; D005978:Glutathione / Q000097:blood*; D006801:Humans; D008297:Male; D012727:Sex Characteristics; D055815:Young Adult
chemical_list: D004151:Dipeptides; D005978:Glutathione; D003545:Cysteine; C017341:gamma-glutamylcysteine
keywords: ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate; gender difference; glutathione; human serum; γ-glutamylcysteine