title: Elucidation of hydration dynamics of locust bean gum-collagen composites by impedance and thermoporometry.
abstract: The intricacy of the different parameters involved in the hydration dynamics of collagen influences its performance as biomaterials. This work presents the molecular motions of collagen originating from the solvents and locust bean gum (LBG), which reveal the changes in solvation dynamics of the biopolymers affecting the surface as well as interfacial properties. Water, as a probe liquid bound in collagen has been investigated using a combination of thermoporometry, ATR-FTIR, circular dichroic spectroscopy, dielectric spectroscopy and SEM to explore the influence of LBG on collagen with respect to static and dynamic behaviour. The relaxation process of collagen in the frequency range of 0.01 Hz to 10(5)Hz and thermoporometry results indicate that the interfacial hydration dynamics are dependent on the applied concentration of LBG. This investigation explicitly reflects the rearrangements of the structural water clusters around the charged amino acids of collagen. These results can be employed to redesign the approach towards the development of collagen based biomaterials.
authors: Chemical Laboratory, Central Leather Research Institute, Council of Scientific and Industrial Research, Adyar, Chennai 600020, India.
authors: Ivy
authors: I
authors: Kanungo
authors: Chemical Laboratory, Central Leather Research Institute, Council of Scientific and Industrial Research, Adyar, Chennai 600020, India.
authors: Nishter Nishad
authors: NN
authors: Fathima
authors: Chemical Laboratory, Central Leather Research Institute, Council of Scientific and Industrial Research, Adyar, Chennai 600020, India. Electronic address: clrichem@mailcity.com.
authors: Raghava Rao
authors: RR
authors: Jonnalagadda
authors: Chemical Laboratory, Central Leather Research Institute, Council of Scientific and Industrial Research, Adyar, Chennai 600020, India.
authors: Balachandran
authors: B
authors: Unni Nair
mesh_terms: D003094:Collagen / Q000737:chemistry*; D005685:Galactans / Q000737:chemistry*; D008351:Mannans / Q000737:chemistry*; D015394:Molecular Structure; D053149:Plant Gums / Q000737:chemistry*; D013816:Thermodynamics; D014867:Water / Q000737:chemistry*
chemical_list: D005685:Galactans; D008351:Mannans; D053149:Plant Gums; D014867:Water; D003094:Collagen; C017471:locust bean gum
keywords: Collagen; Hydration; Impedance; Locust bean gum; Thermoporometry