title: Hypoxia-induced angiogenesis and capillary density determination.
abstract: Chronic exposure to moderate hypoxia elicits structural and functional changes in the microvascular network of the mammalian CNS. Hypoxia-induced angiogenesis can be elicited and studied by a relatively simple experimental method. Rats or mice can be exposed to mild hypoxia in a hypobaric chamber, or alternatively in a normobaric hypoxia chamber. After 3 weeks, the animals are perfused and fixed, the brain removed, and paraffin embedded and sectioned at 5 μm. The sections are stained for the blood-brain barrier glucose transporter (GLUT-1) by immunohistochemistry, capillary profiles identified and counted as a measure of angiogenesis.
authors: Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, OH, USA.
authors: Constantinos P
authors: CP
authors: Tsipis
authors: Xiaoyan
authors: X
authors: Sun
authors: Kui
authors: K
authors: Xu
authors: Joseph C
authors: JC
authors: Lamanna
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015687:Cell Hypoxia; D002540:Cerebral Cortex / Q000098:blood supply*; D051272:Glucose Transporter Type 1 / Q000378:metabolism; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D055806:Microvessels / Q000502:physiology*; D018919:Neovascularization, Physiologic; D016612:Paraffin Embedding; D051381:Rats; D017208:Rats, Wistar; D016707:Tissue Fixation
chemical_list: D015415:Biomarkers; D051272:Glucose Transporter Type 1; C495678:Slc2a1 protein, mouse