title: Implementation of a simplified method of islet isolation for allogeneic islet transplantation in cynomolgus monkeys.
abstract: OBJECTIVE
  The present study describes a simple and cost-effective islet isolation procedure. Using this method, allogeneic islets reverse diabetes in cynomolgus monkeys.
  
  
  METHODS
  Pancreatic tissue from 11 cynomolgus monkeys were digested, collected, and purified using a simplified method. Islet quantification, purity, viability, and glucose static incubation were conducted immediately after isolation. Five streptozotocin-induced monkeys with diabetes were transplanted intrahepatically, and liver biopsies from 3 of these monkeys were taken at different time points for histologic study.
  
  
  RESULTS
  The mean (SD) of viability, purity, and static glucose incubation stimulation index were 94.4% (2.3%), 91.8% (3.4%), and 2.6 (1.7), respectively. Monkeys who received a mean (SD) dose of 19,968 (2273) islet equivalent per kilogram (n = 4) from 2 to 3 donors who achieved prolonged normoglycemia (57-232 days), whereas the single monkey who received an islet dose of 8000 islet equivalent per kilogram did not experience diabetes reversal. Immunohistochemical assessment of the liver biopsies taken from the monkeys with normoglycemia revealed an insulin- and glucagon-positive islet graft for up to 6 months with minimal peri-islet inflammatory infiltration.
  
  
  CONCLUSIONS
  This study demonstrates that cynomolgus monkey islets can be successfully and efficiently harvested using a simple isolation method, and these islets can restore normoglycemia in monkeys with diabetes.
authors: From the Division of Transplantation/Surgery, University of Illinois at Chicago, Chicago, IL.
authors: Meirigeng
authors: M
authors: Qi
authors: Yong
authors: Y
authors: Wang
authors: Kjetil
authors: K
authors: Formo
authors: Enza
authors: E
authors: Marchese
authors: Shusen
authors: S
authors: Wang
authors: James J
authors: JJ
authors: McGarrigle
authors: Matthew A
authors: MA
authors: Bochenek
authors: Katie
authors: K
authors: Kinzer
authors: Pilar
authors: P
authors: Vaca
authors: Maureen
authors: M
authors: Davis
authors: Sang Joon
authors: SJ
authors: Ahn
authors: Kevin
authors: K
authors: Chang
authors: Amelia
authors: A
authors: Bartholomew
authors: Enrico
authors: E
authors: Benedetti
authors: José
authors: J
authors: Oberholzer
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002096:C-Peptide / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000601:surgery*; D005260:Female; D005951:Glucose Tolerance Test; D007515:Islets of Langerhans; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D008252:Macaca fascicularis; D008297:Male; D013997:Time Factors; D020858:Tissue and Organ Harvesting / Q000379:methods*; D014184:Transplantation, Homologous; D016896:Treatment Outcome
chemical_list: D001786:Blood Glucose; D002096:C-Peptide