title: Physalis alkekengi carotenoidic extract inhibitor of soybean lipoxygenase-1 activity.
abstract: The aim of this study was to evaluate the effect of the carotenoidic saponified extract of Physalis alkekengi sepals (PA) towards the lipoxygenase (LOX) oxidation of linoleic acid. Lipoxygenase activity in the presence of carotenoids, standard and from extract, was followed by its kinetic behaviour determining the changes in absorption at 234 nm. The standard carotenoids used were β-carotene (β-car), lutein (Lut), and zeaxanthin (Zea). The calculated enzymatic specific activity (ESA) after 600 s of reaction proves that PA carotenoidic extract has inhibitory effect on LOX oxidation of linoleic acid. A longer polyenic chain of carotenoid structure gives a higher ESA during the first reaction seconds. This situation is not available after 600 s of reaction and may be due to a destruction of this structure by cooxidation of carotenoids, besides the classical LOX reaction. The PA carotenoidic extract inhibiting the LOX-1 reaction can be considered a source of lipoxygenase inhibitors.
authors: Laboratory of Animal Biology, National Research Development Institute for Animal Biology and Nutrition Baloteşti (IBNA), Calea Bucureşti Nr. 1, Baloteşti, 077015 Ilfov, Romania.
authors: Veronica Sanda
authors: VS
authors: Chedea
authors: University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, 3-5 Manastur Street, 400372 Cluj-Napoca, Romania.
authors: Adela
authors: A
authors: Pintea
authors: University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, 3-5 Manastur Street, 400372 Cluj-Napoca, Romania.
authors: Andrea
authors: A
authors: Bunea
authors: Department of Functional Genomics and Experimental Pathology, Chiricuta Cancer Institute, 400015 Cluj-Napoca, Romania.
authors: Cornelia
authors: C
authors: Braicu
authors: University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, 3-5 Manastur Street, 400372 Cluj-Napoca, Romania.
authors: Andreea
authors: A
authors: Stanila
authors: University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, 3-5 Manastur Street, 400372 Cluj-Napoca, Romania.
authors: Carmen
authors: C
authors: Socaciu
mesh_terms: D002338:Carotenoids / Q000737:chemistry / Q000494:pharmacology*; D008084:Lipoxygenase / Q000187:drug effects* / Q000378:metabolism*; D016859:Lipoxygenase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D032321:Physalis / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D016859:Lipoxygenase Inhibitors; D010936:Plant Extracts; D002338:Carotenoids; C101084:lipoxygenase L-1; D008084:Lipoxygenase