title: Polyurethane-based scaffolds for myocardial tissue engineering.
abstract: Bi-layered scaffolds with a 0°/90° lay-down pattern were prepared by melt-extrusion additive manufacturing (AM) using a poly(ester urethane) (PU) synthesized from poly(ε-caprolactone) diol, 1,4-butandiisocyanate and l-lysine ethyl ester dihydrochloride chain extender. Rheological analysis and differential scanning calorimetry of the starting material showed that compression moulded PU films were in the molten state at a higher temperature than 155°C. The AM processing temperature was set at 155°C after verifying the absence of PU thermal degradation phenomena by isothermal thermogravimetry analysis and rheological characterization performed at 165°C. Scaffolds highly reproduced computer-aided design geometry and showed an elastomeric-like behaviour which is promising for applications in myocardial regeneration. PU scaffolds supported the adhesion and spreading of human cardiac progenitor cells (CPCs), whereas they did not stimulate CPC proliferation after 1-14 days culture time. In the future, scaffold surface functionalization with bioactive peptides/proteins will be performed to specifically guide CPC behaviour.
authors: Department of Mechanical and Aerospace Engineering , Politecnico di Torino , Corso Duca degli Abruzzi 24, Turin , Italy.
authors: Valeria
authors: V
authors: Chiono
authors: Tissue Engineering Laboratory , Università 'Campus Bio-Medico di Roma' , Via Alvaro del Portillo 21, Rome , Italy.
authors: Pamela
authors: P
authors: Mozetic
authors: Department of Mechanical and Aerospace Engineering , Politecnico di Torino , Corso Duca degli Abruzzi 24, Turin , Italy.
authors: Monica
authors: M
authors: Boffito
authors: Department of Mechanical and Aerospace Engineering , Politecnico di Torino , Corso Duca degli Abruzzi 24, Turin , Italy.
authors: Susanna
authors: S
authors: Sartori
authors: Department of Mechanical and Aerospace Engineering , Politecnico di Torino , Corso Duca degli Abruzzi 24, Turin , Italy.
authors: Emilia
authors: E
authors: Gioffredi
authors: Department of Mechanical and Aerospace Engineering , Politecnico di Torino , Corso Duca degli Abruzzi 24, Turin , Italy.
authors: Antonella
authors: A
authors: Silvestri
authors: Tissue Engineering Laboratory , Università 'Campus Bio-Medico di Roma' , Via Alvaro del Portillo 21, Rome , Italy.
authors: Alberto
authors: A
authors: Rainer
authors: Tissue Engineering Laboratory , Università 'Campus Bio-Medico di Roma' , Via Alvaro del Portillo 21, Rome , Italy.
authors: Sara Maria
authors: SM
authors: Giannitelli
authors: Tissue Engineering Laboratory , Università 'Campus Bio-Medico di Roma' , Via Alvaro del Portillo 21, Rome , Italy.
authors: Marcella
authors: M
authors: Trombetta
authors: Department of Public Health , University of Naples 'Federico II' , Via Pansini 5, Naples , Italy.
authors: Daria
authors: D
authors: Nurzynska
authors: Department of Public Health , University of Naples 'Federico II' , Via Pansini 5, Naples , Italy.
authors: Franca
authors: F
authors: Di Meglio
authors: Department of Public Health , University of Naples 'Federico II' , Via Pansini 5, Naples , Italy.
authors: Clotilde
authors: C
authors: Castaldo
authors: Department of Public Health , University of Naples 'Federico II' , Via Pansini 5, Naples , Italy.
authors: Rita
authors: R
authors: Miraglia
authors: Department of Public Health , University of Naples 'Federico II' , Via Pansini 5, Naples , Italy.
authors: Stefania
authors: S
authors: Montagnani
authors: Department of Mechanical and Aerospace Engineering , Politecnico di Torino , Corso Duca degli Abruzzi 24, Turin , Italy.
authors: Gianluca
authors: G
authors: Ciardelli
keywords: additive manufacturing; cardiac progenitor cells; myocardial tissue engineering; polyurethane