title: Interplays between nitric oxide and reactive oxygen species in cryptogein signalling.
abstract: Nitric oxide (NO) has many functions in plants. Here, we investigated its interplays with reactive oxygen species (ROS) in the defence responses triggered by the elicitin cryptogein. The production of NO induced by cryptogein in tobacco cells was partly regulated through a ROS-dependent pathway involving the NADPH oxidase NtRBOHD. In turn, NO down-regulated the level of H2O2. Both NO and ROS synthesis appeared to be under the control of type-2 histone deacetylases acting as negative regulators of cell death. Occurrence of an interplay between NO and ROS was further supported by the finding that cryptogein triggered a production of peroxynitrite (ONOO(-)). Next, we showed that ROS, but not NO, negatively regulate the intensity of activity of the cryptogein-induced protein kinase NtOSAK. Furthermore, using a DNA microarray approach, we identified 15 genes early induced by cryptogein via NO. A part of these genes was also modulated by ROS and encoded proteins showing sequence identity to ubiquitin ligases. Their expression appeared to be negatively regulated by ONOO(-), suggesting that ONOO(-) mitigates the effects of NO and ROS. Finally, we provided evidence that NO required NtRBOHD activity for inducing cell death, thus confirming previous assumption that ROS channel NO through cell death pathways.
authors: INRA, UMR 1347 Agroécologie, Pôle Mécanisme et Gestion des Interactions Plantes-Microorganismes - ERL CNRS 6300, 17 rue Sully, BP 86510, 21065, Dijon cédex, France.
authors: Anna
authors: A
authors: Kulik
authors: Elodie
authors: E
authors: Noirot
authors: Vincent
authors: V
authors: Grandperret
authors: Stéphane
authors: S
authors: Bourque
authors: Jérôme
authors: J
authors: Fromentin
authors: Pauline
authors: P
authors: Salloignon
authors: Caroline
authors: C
authors: Truntzer
authors: Grażyna
authors: G
authors: Dobrowolska
authors: Françoise
authors: F
authors: Simon-Plas
authors: David
authors: D
authors: Wendehenne
mesh_terms: D005656:Fungal Proteins / Q000378:metabolism* / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism; D008954:Models, Biological; D009569:Nitric Oxide / Q000378:metabolism*; D030421:Peroxynitrous Acid / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D013535:Suspensions; D014026:Nicotiana / Q000166:cytology / Q000187:drug effects
chemical_list: D005656:Fungal Proteins; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D013535:Suspensions; C061032:cryptogein protein, Phytophthora cryptogea; D030421:Peroxynitrous Acid; D009569:Nitric Oxide; D006861:Hydrogen Peroxide
keywords: NADPH oxidase; SnRK2 protein kinase.; cell death; cryptogein; defence responses; histone deacetylase; peroxynitrite