title: Modified formalin-ether concentration technique for diagnosis of human strongyloidiasis.
abstract: We compared the efficacy and applicability of a modified formalin-ether concentration technique (M-FECT) to the conventional FECT (C-FECT) and the agar plate culture (APC) method for the detection of Strongyloides stercoralis larvae. For this purpose, we used 600 human fecal specimens collected in an endemic area of southern Thailand. In the M-FECT, we used 2 layers of wire meshes, instead of gauze, to avoid the loss by absorption/adhesion of larvae to the gauze during filtration, and we reduced the exposure time of S. stercoralis larvae in stool samples to formalin. By such simple modifications, the efficacy of M-FECT has become comparable to APC and was much better than that of C-FECT for the diagnosis of strongyloidiasis.
authors: Department of Medical Technology, School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat, Thailand.
authors: Witthaya
authors: W
authors: Anamnart
authors: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Pewpan M
authors: PM
authors: Intapan
authors: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Wanchai
authors: W
authors: Maleewong
mesh_terms: D000818:Animals; D004986:Ether; D005243:Feces / Q000469:parasitology; D005557:Formaldehyde; D006801:Humans; D010274:Parasitology / Q000379:methods*; D013048:Specimen Handling / Q000379:methods*; D017171:Strongyloides stercoralis / Q000302:isolation & purification*; D013322:Strongyloidiasis / Q000175:diagnosis*; D013785:Thailand
chemical_list: D004986:Ether; D005557:Formaldehyde
keywords: Strongyloides stercoralis; agar plate culture; diagnosis; feces; formalin-ether concentration technique