title: Alginate immobilization of Escherichia coli MTCC 1652 whole cells for bioconversion of glycyrrhizinic acid and into 18-beta glycyrrhetinic acid.
abstract: Microbial biotransformation of Glycyrrhizinic acid (GL) into 18-beta Glycyrrhetinic Acid (GA) was achieved using Escherichia coli MTCC 1652 whole cell. The E. coli whole cell was immobilized by entrapment method within calcium alginate beads using cell suspension of equal volume with sodium alginate 8%. The pH of solution, reaction volume and % of GL were optimized during the immobilization procedure and optimum pH 6.5, reaction volume of 4 mL and at 3% GL concentration for 12 h of incubation time showed highest concentration of GA (72.649 microg mL(-1)) with 76% bioconversion of GL to GA. Under optimized condition the immobilized cell produces 58.663 microg per mL of GA in licorice root extract containing 95.118 microg of GL per mL of the extract with 61% conversion at 12 h.
authors: Microbial and Pharmaceutical Biotechnology Laboratory, Centre for Advanced Research in Pharmaceutical Science, Faculty of Pharmacy, Jamia Hamdard, New Delhi-1 10062, India.
authors: M
authors: M
authors: Ahmad
authors: Microbial and Pharmaceutical Biotechnology Laboratory, Centre for Advanced Research in Pharmaceutical Science, Faculty of Pharmacy, Jamia Hamdard, New Delhi-1 10062, India.
authors: B P
authors: BP
authors: Panda
mesh_terms: D000464:Alginates / Q000737:chemistry*; D001711:Biotransformation; D004926:Escherichia coli / Q000378:metabolism*; D020723:Glucuronic Acid / Q000737:chemistry; D006034:Glycyrrhetinic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D006035:Glycyrrhiza; D019695:Glycyrrhizic Acid / Q000378:metabolism*; D006603:Hexuronic Acids / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D010936:Plant Extracts / Q000378:metabolism*; D018517:Plant Roots; D013997:Time Factors
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D010936:Plant Extracts; C119129:18alpha-glycyrrhetinic acid; D019695:Glycyrrhizic Acid; D020723:Glucuronic Acid; D006034:Glycyrrhetinic Acid