title: Saccharification behavior of cellulose acetate during enzymatic processing for microbial ethanol production.
abstract: This study was conducted to realize the potential application of cellulose acetate to enzymatic processing, followed by microbial ethanol fermentation. To eliminate the effect of steric hindrance of acetyl groups on the action of cellulase, cellulose acetate was subjected to deacetylation in the presence of 1N sodium hydroxide and a mixture of methanol/acetone, yielding 88.8-98.6% at 5-20% substrate loadings during a 48h saccharification at 50°C. Ethanol fermentation using Saccharomyces cerevisiae attained a high yield of 92.3% from the initial glucose concentration of 44.2g/L; however, a low saccharification yield was obtained at 35°C, decreasing efficiency during simultaneous saccharification and fermentation (SSF). Presaccharification at 50°C prior to SSF without increasing the total process time attained the ethanol titers of 19.8g/L (5% substrate), 38.0g/L (10% substrate), 55.9g/L (15% substrate), and 70.9g/L (20% substrate), which show a 12.0-16.2% improvement in ethanol yield.
authors: Bio-energy Corporation, Research and Development Laboratory, 2-9-7 Minaminanamatsu, Amagasaki 660-0053, Japan.
authors: Shinji
authors: S
authors: Hama
authors: Bio-energy Corporation, Research and Development Laboratory, 2-9-7 Minaminanamatsu, Amagasaki 660-0053, Japan.
authors: Kohsuke
authors: K
authors: Nakano
authors: Bio-energy Corporation, Research and Development Laboratory, 2-9-7 Minaminanamatsu, Amagasaki 660-0053, Japan.
authors: Kaoru
authors: K
authors: Onodera
authors: Bio-energy Corporation, Research and Development Laboratory, 2-9-7 Minaminanamatsu, Amagasaki 660-0053, Japan.
authors: Masashi
authors: M
authors: Nakamura
authors: Bio-energy Corporation, Research and Development Laboratory, 2-9-7 Minaminanamatsu, Amagasaki 660-0053, Japan.
authors: Hideo
authors: H
authors: Noda
authors: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan. Electronic address: akondo@kobe-u.ac.jp.
authors: Akihiko
authors: A
authors: Kondo
mesh_terms: D000107:Acetylation; D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000031:analogs & derivatives* / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
chemical_list: C005062:acetylcellulose; D000431:Ethanol; D002482:Cellulose; D002480:Cellulase; D005947:Glucose
keywords: Acetyl cellulose; Biorefinery; Cellulase; Deacetylation; Microbial fermentation