title: Single-walled carbon nanohorn (SWNH) aggregates inhibited proliferation of human liver cell lines and promoted apoptosis, especially for hepatoma cell lines.
abstract: Single-walled carbon nanohorns (SWNHs) may be useful as carriers for anticancer drugs due to their particular structure. However, the interactions between the material itself and cancerous or normal cells have seldom been studied. To address this problem, the effects of raw SWNH material on the biological functions of human liver cell lines were studied. Our results showed that unmodified SWNHs inhibited mitotic entry, growth, and proliferation of human liver cell lines and promoted their apoptosis, especially in hepatoma cell lines. Individual spherical SWNH particles were found inside the nuclei of human hepatoma HepG2 cells and the lysosomes of normal human liver L02 cells, implying that SWNH particles could penetrate into human liver cells_and the different interacted mechanisms on human normal cell lines compared to hepatoma cell lines. Further research on the mechanisms and application in treatment of hepatocellular carcinoma with SWNHs is needed.
authors: Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing, People's Republic of China.
authors: Jinqian
authors: J
authors: Zhang
authors: Department of Nephrology, Beijing Children's Hospital, Capital Medical University, Beijing, People's Republic of China.
authors: Qiang
authors: Q
authors: Sun
authors: Department of Hematology, Chinese PLA (People's Liberation Army) General Hospital, Beijing, People's Republic of China.
authors: Jian
authors: J
authors: Bo
authors: Department of General Surgery, The Second People's Hospital of Guangdong Province, Southern Medical University, Guangzhou, People's Republic of China.
authors: Rui
authors: R
authors: Huang
authors: Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing, People's Republic of China.
authors: Mengran
authors: M
authors: Zhang
authors: Department of General Surgery, The Second People's Hospital of Guangdong Province, Southern Medical University, Guangzhou, People's Republic of China.
authors: Zhenglin
authors: Z
authors: Xia
authors: Department of Neurobiology, Beijing Institute for Brain Disorders, Beijing Center of Neural Regeneration and Repair, Key Laboratory for Neurodegenerative Diseases of the Ministry of Education, Capital Medical University, Beijing, People's Republic of China.
authors: Lili
authors: L
authors: Ju
authors: Department of General Surgery, The Second People's Hospital of Guangdong Province, Southern Medical University, Guangzhou, People's Republic of China.
authors: Guoan
authors: G
authors: Xiang
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004337:Drug Carriers / Q000494:pharmacology / Q000633:toxicity; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000473:pathology; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D050997:Nanomedicine; D037742:Nanotubes, Carbon / Q000633:toxicity
chemical_list: D004337:Drug Carriers; D037742:Nanotubes, Carbon
keywords: SWNHs; cell apoptosis; cell proliferation; hepatocellular carcinoma; lysosome; nucleus; single-walled carbon nanohorns