title: The polysaccharides from fermented Ganoderma lucidum mycelia induced miRNAs regulation in suppressed HepG2 cells.
abstract: Medicinal mushroom polysaccharides such as Ganoderma lucidum polysaccharides (GLPs) have been commonly hypothesized to suppress tumor cells proliferation through immune effects. To verify this hypothesis through investigating comprehensive miRNA expression in polysaccharide treated cancer cells, an anticancer mycelia GLP was employed to disclose miRNA differential expression of human hepatocarcinoma cells (HepG2), by using a miRNA microarray assay based on Sanger miR-Base Release 16. The experiment and the analysis result indicates that among the 61 differential expressed miRNAs (p ≤ 0.01), 17 of them were regulated significantly. GLP can inhibit HepG2 cells directly through regulation of hepatocarcinoma genes. A newly found miR-3131 exhibited the strongest upregulation (92-folds, Log2 = 6.53, p = 0.000016). The miRNAs responded synergistically in both hepatocarcinoma and immune-related aspects.
authors: State Key Laboratory of Food Science and Technology, School of Chemical Engineering, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, China.
authors: Jie
authors: J
authors: Shen
authors: College of Veterinary Medicine, Gyeongsang National University, Jinju, Gyeongnam 660-701, Republic of Korea.
authors: Hyeon-soo
authors: HS
authors: Park
authors: State Key Laboratory of Food Science and Technology, School of Chemical Engineering, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, China; Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, ON N1G 5C9, Canada. Electronic address: ymxia@jiangnan.edu.cn.
authors: Yong-mei
authors: YM
authors: Xia
authors: College of Veterinary Medicine, Gyeongsang National University, Jinju, Gyeongnam 660-701, Republic of Korea.
authors: Gon-sup
authors: GS
authors: Kim
authors: Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, ON N1G 5C9, Canada.
authors: Steve W
authors: SW
authors: Cui
mesh_terms: D005285:Fermentation; D056945:Hep G2 Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D020940:Reishi / Q000737:chemistry / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; D011134:Polysaccharides
keywords: Antitumor; Ganoderma lucidum; Hepatocarcinoma; Immune; Polysaccharide; miRNA