title: A terrified-sound stress induced proteomic changes in adult male rat hippocampus.
abstract: In this study, we investigated the biochemical mechanisms in the adult rat hippocampus underlying the relationship between a terrified-sound induced psychological stress and spatial learning. Adult male rats were exposed to a terrified-sound stress, and the Morris water maze (MWM) has been used to evaluate changes in spatial learning and memory. The protein expression profile of the hippocampus was examined using two-dimensional gel electrophoresis (2DE), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and bioinformatics analysis. The data from the MWM tests suggested that a terrified-sound stress improved spatial learning. The proteomic analysis revealed that the expression of 52 proteins was down-regulated, while that of 35 proteins were up-regulated, in the hippocampus of the stressed rats. We identified and validated six of the most significant differentially expressed proteins that demonstrated the greatest stress-induced changes. Our study provides the first evidence that a terrified-sound stress improves spatial learning in rats, and that the enhanced spatial learning coincides with changes in protein expression in rat hippocampus.
authors: Department of Genetics and Molecular Biology, Medical School of Xi'an Jiaotong University, 76 Western Yanta Road, Xi'an, Shaanxi 710061, PR China; Key Laboratory of Environment and Disease-Related Gene, Ministry of Education, 76 Western Yanta Road, Xi'an, Shaanxi 710061, PR China.
authors: Juan
authors: J
authors: Yang
authors: Department of Genetics and Molecular Biology, Medical School of Xi'an Jiaotong University, 76 Western Yanta Road, Xi'an, Shaanxi 710061, PR China; Key Laboratory of Environment and Disease-Related Gene, Ministry of Education, 76 Western Yanta Road, Xi'an, Shaanxi 710061, PR China.
authors: Lili
authors: L
authors: Hu
authors: Department of Genetics and Molecular Biology, Medical School of Xi'an Jiaotong University, 76 Western Yanta Road, Xi'an, Shaanxi 710061, PR China; Key Laboratory of Environment and Disease-Related Gene, Ministry of Education, 76 Western Yanta Road, Xi'an, Shaanxi 710061, PR China.
authors: Qiuhua
authors: Q
authors: Wu
authors: Department of Genetics and Molecular Biology, Medical School of Xi'an Jiaotong University, 76 Western Yanta Road, Xi'an, Shaanxi 710061, PR China; Key Laboratory of Environment and Disease-Related Gene, Ministry of Education, 76 Western Yanta Road, Xi'an, Shaanxi 710061, PR China.
authors: Liying
authors: L
authors: Liu
authors: Department of Genetics and Molecular Biology, Medical School of Xi'an Jiaotong University, 76 Western Yanta Road, Xi'an, Shaanxi 710061, PR China; Key Laboratory of Environment and Disease-Related Gene, Ministry of Education, 76 Western Yanta Road, Xi'an, Shaanxi 710061, PR China.
authors: Lingyu
authors: L
authors: Zhao
authors: Department of Genetics and Molecular Biology, Medical School of Xi'an Jiaotong University, 76 Western Yanta Road, Xi'an, Shaanxi 710061, PR China; Key Laboratory of Environment and Disease-Related Gene, Ministry of Education, 76 Western Yanta Road, Xi'an, Shaanxi 710061, PR China.
authors: Xiaoge
authors: X
authors: Zhao
authors: Department of Genetics and Molecular Biology, Medical School of Xi'an Jiaotong University, 76 Western Yanta Road, Xi'an, Shaanxi 710061, PR China; Key Laboratory of Environment and Disease-Related Gene, Ministry of Education, 76 Western Yanta Road, Xi'an, Shaanxi 710061, PR China.
authors: Tusheng
authors: T
authors: Song
authors: Department of Genetics and Molecular Biology, Medical School of Xi'an Jiaotong University, 76 Western Yanta Road, Xi'an, Shaanxi 710061, PR China; Key Laboratory of Environment and Disease-Related Gene, Ministry of Education, 76 Western Yanta Road, Xi'an, Shaanxi 710061, PR China. Electronic address: hchen@mail.xjtu.edu.cn.
authors: Chen
authors: C
authors: Huang
mesh_terms: D000161:Acoustic Stimulation; D000818:Animals; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005239:Fear / Q000502:physiology; D006624:Hippocampus / Q000737:chemistry / Q000502:physiology*; D007525:Isoelectric Focusing; D008297:Male; D008568:Memory / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000502:physiology; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D065853:Spatial Learning / Q000502:physiology; D013315:Stress, Psychological / Q000378:metabolism / Q000503:physiopathology*; D014828:Vocalization, Animal / Q000502:physiology
chemical_list: D009419:Nerve Tissue Proteins
keywords: A terrified-sound stress; Hippocampus; Protein expression; Spatial learning and memory