title: Differential genes in adipocytes induced from polycystic and non-polycystic ovary syndrome-derived human embryonic stem cells.
abstract: We explored the molecular mechanisms of obesity and insulin resistance in patients with polycystic ovary syndrome (PCOS) using a human embryonic stem cell model (hESCs). Three PCOS-derived and one non-PCOS-derived hESC lines were induced into adipocytes, and then total RNA was extracted. The differentially expressed PCOS-derived and non-PCOS-derived adipocytes genes were identified using the Boao Biological human V 2.0 whole genome oligonucleotide microarray. Signals of interest were then validated by real-time PCR. A total of 153 differential genes were expressed of which 91 genes were up-regulated and 62 down-regulated. Nuclear receptor subfamily 0, group B, member 2 (NR0B2) was an up-regulated gene, and the GeneChip CapitalBio® Molecule Annotation System V4.0 indicated that it was associated with obesity and diabetes (Ratio ≥ 2.0X). Multiple genes are involved in PCOS. Nuclear receptor subfamily 0, group B, member 2 may play a role in obesity and insulin resistance in patients with PCOS.
authors: Reproductive Medical Center, The First Affiliated Hospital of Zhengzhou University , Zhengzhou , China .
authors: Fang
authors: F
authors: Wang
authors: Wei-Wei
authors: WW
authors: Liu
authors: Xue-Mei
authors: XM
authors: Chen
authors: Hui-Juan
authors: HJ
authors: Kong
authors: Jing
authors: J
authors: Li
authors: Ying-Pu
authors: YP
authors: Sun
mesh_terms: D017667:Adipocytes / Q000378:metabolism*; D050156:Adipogenesis / Q000235:genetics*; D016022:Case-Control Studies; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D020022:Genetic Predisposition to Disease; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics*; D009765:Obesity / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D011085:Polycystic Ovary Syndrome / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results
keywords: Adipocytes; NR0B2; embryonic stem cells; gene chips; polycystic ovary syndrome