title: A fluorescent probe to detect thiol-containing amino acids in solid tumors.
abstract: Early detecting of cancer is critical to provide proper treatment and to improve survival of patients. Here, we reported a highly sensitive ratiometric (yellow emission (550 nm) to blue emission (496 nm)) fluorescent probe 1 developed for detection of thiol-containing amino acids. This probe successfully eliminates interference from background autofluorescence, and discriminates between human carcinoma and normal cells by detecting intracellular thiol levels in living cells (P < 0.05). Furthermore, the ability of the probe to identify growing tumors by measuring GSH in the tissues as well as in the fresh blood of tumor xenograft mice. Additionally, the ratio of the emission intensity at two different wavelengths can provide quantitative analysis of glutathione (GSH) in the living systems. It suggests that it represents a promising prognostic and diagnostic marker, with extensive and simple potential clinical applications.
authors: Department of Chemistry, Korea University, Seoul 136-701, Republic of Korea.
authors: Wen Xiu
authors: WX
authors: Ren
authors: Division of Biotechnology, Laboratory of Stem Cells and Tissue Regeneration, College of Life Sciences & Biotechnology, Korea University, Seoul 136-713, Republic of Korea.
authors: Jiyou
authors: J
authors: Han
authors: Department of Chemistry, Korea University, Seoul 136-701, Republic of Korea.
authors: Tuhin
authors: T
authors: Pradhan
authors: Division of Biotechnology, Laboratory of Stem Cells and Tissue Regeneration, College of Life Sciences & Biotechnology, Korea University, Seoul 136-713, Republic of Korea.
authors: Ja-Yun
authors: JY
authors: Lim
authors: Department of Chemistry, Korea University, Seoul 136-701, Republic of Korea.
authors: Jae Hong
authors: JH
authors: Lee
authors: Division of Biotechnology, Laboratory of Stem Cells and Tissue Regeneration, College of Life Sciences & Biotechnology, Korea University, Seoul 136-713, Republic of Korea.
authors: Jaehun
authors: J
authors: Lee
authors: Division of Biotechnology, Laboratory of Stem Cells and Tissue Regeneration, College of Life Sciences & Biotechnology, Korea University, Seoul 136-713, Republic of Korea. Electronic address: jhkim@korea.ac.kr.
authors: Jong-Hoon
authors: JH
authors: Kim
authors: Department of Chemistry, Korea University, Seoul 136-701, Republic of Korea. Electronic address: jongskim@korea.ac.kr.
authors: Jong Seung
authors: JS
authors: Kim
mesh_terms: D000596:Amino Acids / Q000097:blood* / Q000737:chemistry*; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000097:blood; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes; D005978:Glutathione / Q000097:blood; D006801:Humans; D009369:Neoplasms / Q000097:blood*
chemical_list: D000596:Amino Acids; D005456:Fluorescent Dyes; D005978:Glutathione
keywords: Blood; GSH measurement; Hepatocellular carcinoma; Ratiometric fluorescence; Tumor tissue