title: Copy number variation in CCND1 gene is implicated in the pathogenesis of sporadic parathyroid carcinoma.
abstract: BACKGROUND
  The molecular bases for parathyroid carcinomas present in conjunction with sporadic primary hyperparathyroidism are not fully elucidated. Gene copy number variations (CNVs) play an important role in tumorigenesis. The aim of the current study was to explore whether the CNVs of specific tumor-associated genes are involved in parathyroid carcinogenesis.
  
  
  METHODS
  A multiplex ligation-dependent probe amplification method was used to compare differences in copy number in 39 common tumor-associated genes among 7 patients with parathyroid carcinoma and 14 age- and sex-matched subjects with parathyroid adenoma.
  
  
  RESULTS
  It was shown that amplification of CCND1, a gene encoding cyclin D1, was more prevalent in parathyroid carcinomas than in adenomas (71 vs. 21 %, p = 0.056). This result was confirmed quantitatively by real-time polymerase chain reaction. Expression of CCND1 mRNA level was significantly higher in carcinomas than in adenomas (p = 0.003). Western blot and immunohistochemical analysis also demonstrated higher expression of CCND1 in carcinoma specimens than in adenoma samples.
  
  
  CONCLUSIONS
  It is thus inferred that gain in copy number of CCND1 is implicated in the molecular pathogenesis of parathyroid carcinoma.
authors: Department of Endocrine and Metabolic Diseases, Rui-jin Hospital, Shanghai Jiao-tong University School of Medicine, Shanghai Institute of Endocrine and Metabolic Diseases, Shanghai Clinical Center for Endocrine and Metabolic Diseases, 197 Rui-jin Er Road, Shanghai, 200025, People's Republic of China.
authors: Lin
authors: L
authors: Zhao
authors: Li-hao
authors: LH
authors: Sun
authors: Dong-mei
authors: DM
authors: Liu
authors: Xiao-yan
authors: XY
authors: He
authors: Bei
authors: B
authors: Tao
authors: Guang
authors: G
authors: Ning
authors: Jian-min
authors: JM
authors: Liu
authors: Hong-yan
authors: HY
authors: Zhao
mesh_terms: D000236:Adenoma / Q000737:chemistry / Q000235:genetics*; D000328:Adult; D000368:Aged; D002277:Carcinoma / Q000737:chemistry / Q000235:genetics*; D019938:Cyclin D1 / Q000032:analysis / Q000235:genetics*; D056915:DNA Copy Number Variations; D005260:Female; D006801:Humans; D049950:Hyperparathyroidism, Primary / Q000150:complications; D008297:Male; D008875:Middle Aged; D060885:Multiplex Polymerase Chain Reaction; D010282:Parathyroid Neoplasms / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D019938:Cyclin D1