title: Potential role of porcine reproductive and respiratory syndrome virus structural protein GP2 in apoptosis inhibition.
abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) is a serious threat to the pork industry, and its pathogenesis needs further investigations. To study the role of two structural proteins of PRRSV in virus-host cells interactions, two stable cell lines (MARC-2a and MARC-N) expressing GP2 and N proteins, respectively, were established. We induced apoptosis in these cells by treating them with staurosporine and found a significant reduction in the number of apoptotic cells in MARC-2a as compared to MARC-N and MARC-145 cells. In addition, we found significantly higher activities of transcriptional factors (NF- κ B and AP-1) in both cell lines as compared to MARC-145 (parent cells). Overall, our data suggest that, although both stable cell lines activate NF- κ B and AP-1, GP2 triggers the antiapoptotic process through an intermediate step that needs to be further investigated.
authors: International Vaccine Center (VIDO-InterVac), Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK, Canada S7N 5E3.
authors: Sujit
authors: S
authors: Pujhari
authors: Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E5.
authors: Tayyba T
authors: TT
authors: Baig
authors: International Vaccine Center (VIDO-InterVac), Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK, Canada S7N 5E3.
authors: Alexander N
authors: AN
authors: Zakhartchouk
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D002460:Cell Line / Q000821:virology; D008460:Meat; D016328:NF-kappa B / Q000235:genetics; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics* / Q000378:metabolism; D019311:Staurosporine / Q000008:administration & dosage; D013552:Swine; D015678:Viral Structural Proteins / Q000235:genetics*
chemical_list: D016328:NF-kappa B; D015678:Viral Structural Proteins; D019311:Staurosporine