title: Monitoring microRNAs using a molecular beacon in CD133+/ CD338+ human lung adenocarcinoma-initiating A549 cells.
abstract: Lung cancer is the most common causes of cancer-related deaths worldwide, and a lack of effective methods for early diagnosis has greatly impacted the prognosis and survival rates of the affected patients. Tumor-initiating cells (TICs) are considered to be largely responsible for tumor genesis, resistance to tumor therapy, metastasis, and recurrence. In addition to representing a good potential treatment target, TICs can provide clues for the early diagnosis of cancer. MicroRNA (miRNA) alterations are known to be involved in the initiation and progression of human cancer, and the detection of related miRNAs in TICs is an important strategy for lung cancer early diagnosis. As Hsa-miR-155 (miR-155) can be used as a diagnostic marker for non-small cell lung cancer (NSCLC), a smart molecular beacon of miR-155 was designed to image the expression of miR-155 in NSCLC cases. TICs expressing CD133 and CD338 were obtained from A549 cells by applying an immune magnetic bead isolation system, and miR-155 was detected using laser-scanning confocal microscopy. We found that intracellular miR- 155 could be successfully detected using smart miR-155 molecular beacons. Expression was higher in TICs than in A549 cells, indicating that miR-155 may play an important role in regulating bio-behavior of TICs. As a non-invasive approach, molecular beacons could be implemented with molecular imaging to diagnose lung cancer at early stages.
authors: Diagnosis and Treatment Center of Cancer, Chengdu Military General Hospital, Chengdu, China E-mail : zhtao269@yeah.net, chenzhtang@yeah.net.
authors: Quan
authors: Q
authors: Yao
authors: Jian-Guo
authors: JG
authors: Sun
authors: Hu
authors: H
authors: Ma
authors: An-Mei
authors: AM
authors: Zhang
authors: Sheng
authors: S
authors: Lin
authors: Cong-Hui
authors: CH
authors: Zhu
authors: Tao
authors: T
authors: Zhang
authors: Zheng-Tang
authors: ZT
authors: Chen
mesh_terms: D000071916:AC133 Antigen; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters / Q000032:analysis; D015703:Antigens, CD / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis*; D002289:Carcinoma, Non-Small-Cell Lung / Q000737:chemistry* / Q000235:genetics; D045744:Cell Line, Tumor; D015870:Gene Expression; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D018189:Immunomagnetic Separation; D008175:Lung Neoplasms / Q000737:chemistry* / Q000235:genetics; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D018613:Microscopy, Confocal; D009363:Neoplasm Proteins / Q000032:analysis; D014411:Neoplastic Stem Cells / Q000737:chemistry*; D010455:Peptides / Q000032:analysis
chemical_list: C116614:ABCG2 protein, human; D000071916:AC133 Antigen; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters; D015703:Antigens, CD; D014408:Biomarkers, Tumor; D006023:Glycoproteins; C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D009363:Neoplasm Proteins; C000606023:PROM1 protein, human; D010455:Peptides