title: A novel hapten and monoclonal-based enzyme-linked immunosorbent assay for sulfonamides in edible animal tissues.
abstract: For high-throughput monitoring of the residues of sulfonamides (SAs) in edible animal tissues, a novel hapten and monoclonal-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed. The novel hapten was synthesized and conjugated to carrier protein as immunogen. The spleen cells of the inoculated mice expressing group-specificity against SAs were fused. The obtained monoclonal antibody 4E5 showed the cross-reactivity (CR) to 16 structurally different SAs. Based on this antibody, an optimised icELISA protocol was carried out with only phosphate-buffered saline for the fast extraction of SAs in the tissues. The limits of detection of SAs in chicken ranged from 1.5 to 22.3μgkg(-1). The recoveries were 70.6-121% with less than 24.1% relative standard deviation. The developed ic-ELISA showed a good correlation with high performance liquid chromatography. It would be a useful tool for the screening of residues of SAs in edible animal tissues.
authors: National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors: Qi
authors: Q
authors: Zhou
authors: National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors: Dapeng
authors: D
authors: Peng
authors: National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors: Yulian
authors: Y
authors: Wang
authors: National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors: Yuanhu
authors: Y
authors: Pan
authors: National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors: Dan
authors: D
authors: Wan
authors: National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors: Xiya
authors: X
authors: Zhang
authors: National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan, Hubei 430070, China. Electronic address: yuan5802@mail.hzau.edu.cn.
authors: Zonghui
authors: Z
authors: Yuan
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002645:Chickens; D004350:Drug Residues / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D005260:Female; D005506:Food Contamination / Q000032:analysis*; D006241:Haptens / Q000737:chemistry; D008460:Meat / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000737:chemistry*; D013449:Sulfonamides / Q000032:analysis*
chemical_list: D000900:Anti-Bacterial Agents; D006241:Haptens; D013449:Sulfonamides
keywords: Edible animal tissues; Hapten; Indirect competitive enzyme-linked immunosorbent assay; Monoclonal antibody; Sulfonamides