title: Processing-body movement in Arabidopsis depends on an interaction between myosins and DECAPPING PROTEIN1.
abstract: Processing (P)-bodies are cytoplasmic RNA protein aggregates responsible for the storage, degradation, and quality control of translationally repressed messenger RNAs in eukaryotic cells. In mammals, P-body-related RNA and protein exchanges are actomyosin dependent, whereas P-body movement requires intact microtubules. In contrast, in plants, P-body motility is actin based. In this study, we show the direct interaction of the P-body core component DECAPPING PROTEIN1 (DCP1) with the tails of different unconventional myosins in Arabidopsis (Arabidopsis thaliana). By performing coexpression studies with AtDCP1, dominant-negative myosin fragments, as well as functional full-length myosin XI-K, the association of P-bodies and myosins was analyzed in detail. Finally, the combination of mutant analyses and characterization of P-body movement patterns showed that myosin XI-K is essential for fast and directed P-body transport. Together, our data indicate that P-body movement in plants is governed by myosin XI members through direct binding to AtDCP1 rather than through an adapter protein, as known for membrane-coated organelles. Interspecies and intraspecies interaction approaches with mammalian and yeast protein homologs suggest that this mechanism is evolutionarily conserved among eukaryotes.
authors: Botanical Institute, Biocenter, Cologne University, Cologne 50674, Germany.
authors: Alexandra
authors: A
authors: Steffens
authors: Benjamin
authors: B
authors: Jaegle
authors: Achim
authors: A
authors: Tresch
authors: Martin
authors: M
authors: Hülskamp
authors: Marc
authors: M
authors: Jakoby
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism*; D022083:Cytoplasmic Structures / Q000378:metabolism*; D004722:Endoribonucleases / Q000378:metabolism*; D005453:Fluorescence; D006801:Humans; D008322:Mammals; D009068:Movement; D009154:Mutation / Q000235:genetics; D009218:Myosins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013045:Species Specificity
chemical_list: D000199:Actins; D029681:Arabidopsis Proteins; D011993:Recombinant Fusion Proteins; C523559:myosin XIK protein, Arabidopsis; D004722:Endoribonucleases; C518493:Dcp1 protein, Arabidopsis; D009218:Myosins