title: Identification of Streptomyces coelicolor M145 genomic region involved in biosynthesis of teichulosonic acid-cell wall glycopolymer.
abstract: The cell wall of the model actinomycete Streptomyces coelicolor M145 has recently been shown to contain the novel glycopolymer teichulosonic acid. The major building block of this polymer is 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (Kdn), suggesting initial clues about the genetic control of biosynthesis of this cell wall component. Here, through genome mining and gene knockouts, we demonstrate that the sco4879-sco4882 genomic region of S. coelicolor M145 is necessary for biosynthesis of teichulosonic acid. Specifically, mutants carrying individual knockouts of sco4879, sco4880 and sco4881 genes do not produce Kdn-containing glycopolymer and instead accumulate the minor cell wall component poly(diglycosyl 1-phosphate). Our studies provide evidence that this region is at least partly responsible for biosynthesis of Kdn, whereas flanking genes might control the other steps of teichulosonic acid formation.
authors: Department of Genetics and Biotechnology, Ivan Franko National University, Lviv, 4 Hrushevskoho st, Lviv, 79005, Ukraine.
authors: Bohdan
authors: B
authors: Ostash
authors: Alexander
authors: A
authors: Shashkov
authors: Galina
authors: G
authors: Streshinskaya
authors: Elena
authors: E
authors: Tul'skaya
authors: Lidiya
authors: L
authors: Baryshnikova
authors: Andrey
authors: A
authors: Dmitrenok
authors: Yuriy
authors: Y
authors: Dacyuk
authors: Victor
authors: V
authors: Fedorenko
mesh_terms: D002473:Cell Wall / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D057225:Data Mining; D009682:Magnetic Resonance Spectroscopy; D016254:Mutagenesis, Insertional; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D048372:Streptomyces coelicolor / Q000235:genetics / Q000378:metabolism*; D013400:Sugar Acids / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D011135:Polysaccharides, Bacterial; D013400:Sugar Acids; C050008:3-deoxyglycero-galacto-nonulosonic acid