title: Weak power frequency magnetic field acting similarly to EGF stimulation, induces acute activations of the EGFR sensitive actin cytoskeleton motility in human amniotic cells.
abstract: In this article, we have examined the motility-related effects of weak power frequency magnetic fields (MFs) on the epidermal growth factor receptor (EGFR)-sensitive motility mechanism, including the F-actin cytoskeleton, growth of invasive protrusions and the levels of signal molecules in human amniotic epithelial (FL) cells. Without extracellular EGF stimulation, the field stimulated a large growth of new protrusions, especially filopodia and lamellipodia, an increased population of vinculin-associated focal adhesions. And, an obvious reduction of stress fiber content in cell centers was found, corresponding to larger cell surface areas and decreased efficiency of actin assembly of FL cells in vitro, which was associated with a decrease in overall F-actin content and special distributions. These effects were also associated with changes in protein content or distribution patterns of the EGFR downstream motility-related signaling molecules. All of these effects are similar to those following epidermal growth factor (EGF) stimulation of the cells and are time dependent. These results suggest that power frequency MF exposure acutely affects the migration/motility-related actin cytoskeleton reorganization that is regulated by the EGFR-cytoskeleton signaling pathway. Therefore, upon the MF exposure, cells are likely altered to be ready to transfer into a state of migration in response to the stimuli.
authors: Physics Department, East China Normal University, Shanghai, China ; State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai, China.
authors: Xia
authors: X
authors: Wu
authors: Physics Department, East China Normal University, Shanghai, China.
authors: Mei-Ping
authors: MP
authors: Cao
authors: Bioelectromagnetics Laboratory, Zhejiang University, Hangzhou, China.
authors: Yun-Yun
authors: YY
authors: Shen
authors: Physics Department, East China Normal University, Shanghai, China.
authors: Ke-Ping
authors: KP
authors: Chu
authors: Physics Department, East China Normal University, Shanghai, China.
authors: Wu-Bin
authors: WB
authors: Tao
authors: Physics Department, East China Normal University, Shanghai, China.
authors: Wei-Tao
authors: WT
authors: Song
authors: School of Life Sciences, Fudan University, Shanghai, China.
authors: Li-Ping
authors: LP
authors: Liu
authors: Physics Department, East China Normal University, Shanghai, China.
authors: Xiang-Hui
authors: XH
authors: Wang
authors: School of Life Sciences, Fudan University, Shanghai, China.
authors: Yu-Fang
authors: YF
authors: Zheng
authors: Physics Department, East China Normal University, Shanghai, China.
authors: Shu-De
authors: SD
authors: Chen
authors: Bioelectromagnetics Laboratory, Zhejiang University, Hangzhou, China.
authors: Qun-Li
authors: QL
authors: Zeng
authors: Physics Department, East China Normal University, Shanghai, China ; State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai, China.
authors: Ruo-Hong
authors: RH
authors: Xia
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D000650:Amnion / Q000166:cytology / Q000378:metabolism*; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000378:metabolism* / Q000494:pharmacology; D066246:ErbB Receptors / Q000378:metabolism*; D022001:Focal Adhesions / Q000378:metabolism; D006801:Humans; D060526:Magnetic Fields; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology
chemical_list: D004815:Epidermal Growth Factor; C512478:EGFR protein, human; D066246:ErbB Receptors