title: Characterisation of organic and conventional sweet basil leaves using chromatographic and flow-injection mass spectrometric (FIMS) fingerprints combined with principal component analysis.
abstract: Sweet basil, Ocimum basilicum, is one of the most important and wildly used spices and has been shown to have antioxidant, antibacterial, and anti-diarrheal activities. In this study, high performance liquid chromatographic (HPLC) and flow-injection mass spectrometric (FIMS) fingerprinting techniques were used to differentiate organic and conventional sweet basil leaf samples. Principal component analysis (PCA) of the fingerprints indicated that both HPLC and FIMS fingerprints could effectively detect the chemical differences in the organic and conventional sweet basil leaf samples. This study suggested that the organic basil sample contained greater concentrations of almost all the major compounds than its conventional counterpart on a per same botanical weight basis. The FIMS method was able to rapidly differentiate the organic and conventional sweet basil leaf samples (1min analysis time), whereas the HPLC fingerprints provided more information about the chemical composition of the basil samples with a longer analytical time.
authors: Department of Nutrition and Food Science, University of Maryland, College Park, 20742 MD, USA.
authors: Yingjian
authors: Y
authors: Lu
authors: Department of Nutrition and Food Science, University of Maryland, College Park, 20742 MD, USA; Institute of Food and Nutraceutical Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors: Boyan
authors: B
authors: Gao
authors: Food Composition and Methods Development Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, 20705 MD, USA. Electronic address: Pei.Chen@ars.usda.gov.
authors: Pei
authors: P
authors: Chen
authors: Frontier Natural Products Co-op, 3021 78th Street, Norway, IA 52318, USA.
authors: Denys
authors: D
authors: Charles
authors: Department of Nutrition and Food Science, University of Maryland, College Park, 20742 MD, USA; Institute of Food and Nutraceutical Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: liangli.yu@sjtu.edu.cn.
authors: Liangli Lucy
authors: LL
authors: Yu
mesh_terms: D000383:Agriculture; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D058870:Food, Organic / Q000032:analysis; D013058:Mass Spectrometry / Q000379:methods*; D018649:Ocimum basilicum / Q000737:chemistry* / Q000254:growth & development; D058871:Organic Agriculture; D010936:Plant Extracts / Q000032:analysis; D018515:Plant Leaves / Q000737:chemistry* / Q000254:growth & development; D025341:Principal Component Analysis
chemical_list: D010936:Plant Extracts
keywords: Flow injection; HPLC fingerprint; Mass spectral fingerprint; Principal component analysis; Sweet basil (Ocimum basilicum)