title: Clone 13-infected Aedes aegypti salivary components inhibit Rift Valley fever virus pathogenicity.
abstract: Rift Valley fever virus (RVFV) continues to cause large outbreaks among humans and domestic animals in Africa. RVFV Clone 13, a naturally attenuated clone, is a promising vaccine which was used during the 2009-2010 outbreak in South Africa and played a key role in the control of the disease. In this work, we infected Aedes aegypti mosquitoes with RVFV Clone 13 and prepared salivary gland extracts (SGE). C57BL/6-NRJ male mice were infected with a mixture of SGE infected by Clone 13 and the ZH548 RVFV strain. With the injection of increasing doses of Clone 13-infected SGE, all mice were protected. Our results suggest Clone 13 infected SGE contain unique antiviral components able to counteract the replication of RVFV when injected into vertebrates.
authors: Unité de Génétique Moléculaire des Bunyavirus, Institut Pasteur, 25 rue du Dr Roux, Paris, France.
authors: Alain
authors: A
authors: Le Coupanec
authors: Unité de Génétique Moléculaire des Bunyavirus, Institut Pasteur, 25 rue du Dr Roux, Paris, France.
authors: Divya
authors: D
authors: Babin
authors: Unité de Génétique Moléculaire des Bunyavirus, Institut Pasteur, 25 rue du Dr Roux, Paris, France.
authors: Michèle
authors: M
authors: Bouloy
authors: Unité de Génétique Moléculaire des Bunyavirus, Institut Pasteur, 25 rue du Dr Roux, Paris, France; Unité Interactions Moléculaires Flavivirus-Hôtes, Institut Pasteur, 25 rue du Dr Roux, Paris, France. Electronic address: vchoumet@pasteur.fr.
authors: Valérie
authors: V
authors: Choumet
mesh_terms: D000330:Aedes / Q000276:immunology / Q000821:virology*; D000818:Animals; D005260:Female; D008297:Male; D008810:Mice, Inbred C57BL; D012296:Rift Valley fever virus / Q000276:immunology*; D012463:Saliva / Q000276:immunology / Q000821:virology; D014613:Vaccines, Attenuated / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D014613:Vaccines, Attenuated; D014765:Viral Vaccines
keywords: Aedes aegypti; Arbovirus; Clone 13; Pathogenicity; Rift Valley fever virus; Salivary gland