title: Micro-architectural changes in cancellous bone differ in female and male C57BL/6 mice with high-fat diet-induced low bone mineral density.
abstract: The relationship between fat and bone mass at distinct trabecular and cortical skeletal compartments in a high-fat diet (HFD) model was studied. For this, C57BL/6 mice were assigned to four groups of eight animals each. Two groups, each of males and females, received a standard chow diet while the remaining other two groups received the HFD for a period of 10 weeks. Male mice on the HFD were heavier and gained more weight (15·8 %; P<  0·05) v. those on the control diet or when compared with the female rats fed the HFD. We observed an increased lipid profile in both males and females, with significantly higher lipid levels (about 20-25 %; P< 0·01) in males. However, glucose intolerance was more pronounced in females than males on the HFD (about 30 %; P< 0·05). The micro-architectural assessment of bones showed that compared with female mice on the HFD, male mice on the HFD showed more deterioration at the trabecular region. This was corroborated by plasma osteocalcin and carboxy-terminal collagen crosslinks (CTx) levels confirming greater loss in males (about 20 %; P< 0·01). In both sexes cortical bone parameters and strength remained unchanged after 10 weeks of HFD treatment. The direct effect of the HFD on bone at the messenger RNA level in progenitor cells isolated from femoral bone marrow was a significantly increased expression of adipogenic marker genes v. osteogenic genes. Overall, the present data indicate that obesity induced by a HFD aggravates bone loss in the cancellous bone compartment, with a greater loss in males than females, although 10 weeks of HFD treatment did not alter cortical bone mass and strength in both males and females.
authors: Division of Endocrinology, Council of Scientific and Industrial Research (CSIR)-Central Drug Research Institute, Lucknow, India.
authors: Jyoti
authors: J
authors: Gautam
authors: Division of Endocrinology, Council of Scientific and Industrial Research (CSIR)-Central Drug Research Institute, Lucknow, India.
authors: Dharmendra
authors: D
authors: Choudhary
authors: Division of Endocrinology, Council of Scientific and Industrial Research (CSIR)-Central Drug Research Institute, Lucknow, India.
authors: Vikram
authors: V
authors: Khedgikar
authors: Division of Endocrinology, Council of Scientific and Industrial Research (CSIR)-Central Drug Research Institute, Lucknow, India.
authors: Priyanka
authors: P
authors: Kushwaha
authors: Department of Molecular Medicine and Biotechnology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India.
authors: Ravi Shankar
authors: RS
authors: Singh
authors: Division of Endocrinology, Council of Scientific and Industrial Research (CSIR)-Central Drug Research Institute, Lucknow, India.
authors: Divya
authors: D
authors: Singh
authors: Department of Molecular Medicine and Biotechnology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India.
authors: Swasti
authors: S
authors: Tiwari
authors: Division of Endocrinology, Council of Scientific and Industrial Research (CSIR)-Central Drug Research Institute, Lucknow, India.
authors: Ritu
authors: R
authors: Trivedi
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D001835:Body Weight; D015519:Bone Density / Q000235:genetics / Q000502:physiology*; D001854:Bone Marrow Cells / Q000378:metabolism; D003094:Collagen / Q000378:metabolism*; D059305:Diet, High-Fat / Q000009:adverse effects*; D004195:Disease Models, Animal; D005260:Female; D005269:Femur / Q000473:pathology; D015870:Gene Expression; D018149:Glucose Intolerance / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010006:Osteoblasts / Q000166:cytology*; D015675:Osteocalcin / Q000097:blood*; D010024:Osteoporosis / Q000209:etiology*; D012333:RNA, Messenger / Q000378:metabolism; D012737:Sex Factors; D013234:Stem Cells / Q000378:metabolism; D013977:Tibia / Q000473:pathology; D055114:X-Ray Microtomography
chemical_list: D012333:RNA, Messenger; D015675:Osteocalcin; D003094:Collagen; D000469:Alkaline Phosphatase