title: Detection of microvasculature alterations by synchrotron radiation in murine with delayed jellyfish envenomation syndrome.
abstract: Using the tentacle extract (TE) from the jellyfish Cyanea capillata, we have previously established a delayed jellyfish envenomation syndrome (DJES) model, which is meaningful for clinical interventions against jellyfish stings. However, the mechanism of DJES still remains unclear. Thus, this study aimed to explore its potential mechanism by detecting TE-induced microvasculature alterations in vivo and ex vivo. Using a third-generation synchrotron radiation facility, we, for the first time, directly observed the blood vessel alterations induced by jellyfish venom in vivo and ex vivo. Firstly, microvasculature imaging of whole-body mouse in vivo indicated that the small blood vessel branches in the liver and kidney in the TE-treated group, seemed much thinner than those in the control group. Secondly, 3D imaging of kidney ex vivo showed that the kidneys in the TE-treated group had incomplete vascular trees where distal vessel branches were partly missing and disorderly disturbed. Finally, histopathological analysis found that obvious morphological changes, especially hemorrhagic effects, were also present in the TE-treated kidney. Thus, TE-induced microvasculature changes might be one of the important mechanisms of multiple organ dysfunctions in DJES. In addition, the methods we employed here will probably facilitate further studies on developing effective intervention strategies against DJES.
authors: Department of Marine Biotechnology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China.
authors: Beilei
authors: B
authors: Wang
authors: Department of Marine Biotechnology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China.
authors: Bo
authors: B
authors: Zhang
authors: Northern Hospital, General Hospital of PLA Shenyang Military Area Command, Shenyang 110031, China.
authors: Hua
authors: H
authors: Huo
authors: Department of Marine Biotechnology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China.
authors: Tao
authors: T
authors: Wang
authors: Department of Marine Biotechnology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China.
authors: Qianqian
authors: Q
authors: Wang
authors: Department of Cardiovascular Surgery, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors: Yuanlin
authors: Y
authors: Wu
authors: Department of Marine Biotechnology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China.
authors: Liang
authors: L
authors: Xiao
authors: Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, CAS 239 Zhang Heng Road, Pudong District, Shanghai 201204, China.
authors: Yuqi
authors: Y
authors: Ren
authors: Department of Marine Biotechnology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China. Electronic address: lmzhang1969@163.com.
authors: Liming
authors: L
authors: Zhang
mesh_terms: D000818:Animals; D001733:Bites and Stings / Q000000981:diagnostic imaging*; D003063:Cnidaria; D003064:Cnidarian Venoms / Q000633:toxicity*; D008815:Mice, Inbred Strains; D055806:Microvessels / Q000187:drug effects*; D017207:Rats, Sprague-Dawley; D037401:Scyphozoa; D017356:Synchrotrons; D055114:X-Ray Microtomography
chemical_list: D003064:Cnidarian Venoms
keywords: Delayed jellyfish envenomation syndrome; Jellyfish; Micro-computed tomography; Synchrotron radiation; X-ray microangiography