title: Proliferation inhibition and the underlying molecular mechanisms of microRNA-30d in renal carcinoma cells.
abstract: To investigate the inhibitory effects of microRNA-30d (miR-30d) on renal carcinoma cell proliferation and the underlying molecular mechanisms, miR-30d expression in renal cell carcinoma (RCC) specimens was analyzed by quantitative polymerase chain reaction (qPCR). The inhibition of the proliferation of miR-30d on renal carcinoma cells (ACHN cell line) was analyzed by MTT and colony formation assays. The effects of miR-30d on cyclin E2 expression were detected by the luciferase activity of the reporter gene. In addition, the effects of miR-30d on endogenous cyclin E2 expression at the RNA and protein levels were investigated by qPCR and western blot analysis, respectively. Cell cycles were analyzed by flow cytometry. The results showed the following: i) Expression of miR-30d was significantly downregulated in renal carcinoma tissues compared with paraneoplastic tissues; ii) overexpression of miR-30d inhibited renal carcinoma cell proliferation and colony formation; iii) miR-30d inhibited cyclin E2 3' untranslated region-mediated reporter gene expression; and iv) overexpression of miR-30d downregulated endogenous cyclin E2 expression and blocked the cell cycle at the G1phase. In conclusion, miR-30d functions as a tumor suppressor gene in RCC and inhibits renal carcinoma cell proliferation. Cell cycle regulatory factor cyclin E2 is a target gene of miR-30d. miR-30d inhibits renal carcinoma cell proliferation via the regulation of cyclin E2 expression at the post-transcriptional level.
authors: Medical School, Ningbo University, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Ningbo, Zhejiang 315211, P.R. China ; Affiliated Hospital, Ningbo University, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Ningbo, Zhejiang 315211, P.R. China.
authors: Hongsheng
authors: H
authors: Yu
authors: Medical School, Ningbo University, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Ningbo, Zhejiang 315211, P.R. China.
authors: Xialu
authors: X
authors: Lin
authors: Affiliated Hospital, Ningbo University, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Ningbo, Zhejiang 315211, P.R. China.
authors: Fang
authors: F
authors: Wang
authors: Affiliated Hospital, Ningbo University, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Ningbo, Zhejiang 315211, P.R. China.
authors: Burong
authors: B
authors: Zhang
authors: Affiliated Hospital, Ningbo University, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Ningbo, Zhejiang 315211, P.R. China.
authors: Weihua
authors: W
authors: Wang
authors: Medical School, Ningbo University, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Ningbo, Zhejiang 315211, P.R. China.
authors: Hongbo
authors: H
authors: Shi
authors: Medical School, Ningbo University, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Ningbo, Zhejiang 315211, P.R. China.
authors: Baobo
authors: B
authors: Zou
authors: Medical School, Ningbo University, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Ningbo, Zhejiang 315211, P.R. China.
authors: Jinshun
authors: J
authors: Zhao
keywords: cyclin E2; microRNA-30d; proliferation inhibition; renal carcinoma cells; renal cell carcinoma