title: Reproducible surface-enhanced Raman quantification of biomarkers in multicomponent mixtures.
abstract: Direct and quantitative detection of unlabeled glycerophosphoinositol (GroPIns), an abundant cytosolic phosphoinositide derivative, would allow rapid evaluation of several malignant cell transformations. Here we report label-free analysis of GroPIns via surface-enhanced Raman spectroscopy (SERS) with a sensitivity of 200 nM, well below its apparent concentration in cells. Crucially, our SERS substrates, based on lithographically defined gold nanofeatures, can be used to predict accurately the GroPIns concentration even in multicomponent mixtures, avoiding the preliminary separation of individual compounds. Our results represent a critical step toward the creation of SERS-based biosensor for rapid, label-free, and reproducible detection of specific molecules, overcoming limits of current experimental methods.
authors: Institute of Protein Biochemistry, National Research Council , Via P. Castellino 111, 80131 Naples, Italy.
authors: Anna Chiara
authors: AC
authors: De Luca
authors: Peter
authors: P
authors: Reader-Harris
authors: Michael
authors: M
authors: Mazilu
authors: Stefania
authors: S
authors: Mariggiò
authors: Daniela
authors: D
authors: Corda
authors: Andrea
authors: A
authors: Di Falco
mesh_terms: D015415:Biomarkers / Q000032:analysis / Q000737:chemistry; D005990:Glycerol / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D007294:Inositol / Q000737:chemistry; D007295:Inositol Phosphates / Q000032:analysis* / Q000737:chemistry; D055620:Optical Phenomena; D015203:Reproducibility of Results; D013059:Spectrum Analysis, Raman; D013499:Surface Properties; D013997:Time Factors
chemical_list: D015415:Biomarkers; D007295:Inositol Phosphates; C014575:glycerylphosphoinositol; D007294:Inositol; D006046:Gold; D005990:Glycerol