title: Dietary intake of a plant phospholipid/lipid conjugate reduces lung cancer growth and tumor angiogenesis.
abstract: It is well recognized that early detection and cancer prevention are significant armaments in the 'war against cancer'. Changes in lifestyle and diet have significant impact on the global incidence of cancer. For over 30 years, many investigators have studied the concept of chemoprevention. More recently, with the demonstration that antiangiogenic activity reduces tumor growth, the concept of angioprevention has emerged as a novel strategy in the deterrence of cancer development (carcinogenesis). In this study, we utilized a fast growing, highly aggressive murine Lewis lung cancer model to examine the in vivo antitumor effects of a novel, dietary supplement, known as plant phospholipid/lipid conjugate (pPLC). Our goal was to determine if pPLC possessed direct antitumor activity with relatively little toxicity that could be developed as a chemoprevention therapy. We used pPLC directly in this in vivo model due to the lack of aqueous solubility of this novel formulation, which precludes in vitro experimentation. pPLC contains known antioxidants, ferulic acid and lipoic acid, as well as soy sterols, formulated in a unique aqueous-insoluble matrix. The pPLC dietary supplement was shown to suppress in vivo growth of this tumor model by 30%. We also demonstrated a significant decrease in tumor angiogenesis accompanied by increased apoptosis and present preliminary evidence of enhanced expression of the hypoxia-related genes pentraxin-3 and metallothionein-3, by 24.9-fold and 10.9-fold, respectively, compared with vehicle control. These findings lead us to propose using this plant phosolipid/lipid conjugate as a dietary supplement that may be useful in cancer prevention.
authors: Radiation Oncology Branch, National Cancer Institute, Bethesda, MD 20895, USA, sstevenw@mail.nih.gov laurie.shumanmoss@nih.gov.
authors: Laurie A
authors: LA
authors: Shuman Moss
authors: Radiation Oncology Branch, National Cancer Institute, Bethesda, MD 20895, USA.
authors: Sandra
authors: S
authors: Jensen-Taubman
authors: Radiation Oncology Branch, National Cancer Institute, Bethesda, MD 20895, USA, Gettysburg College, Gettysburg, PA 17325, USA and.
authors: Danielle
authors: D
authors: Rubinstein
authors: Conjugated Functional Foods, Hackensack, NJ 07601, USA.
authors: Gary
authors: G
authors: Viole
authors: Radiation Oncology Branch, National Cancer Institute, Bethesda, MD 20895, USA, sstevenw@mail.nih.gov.
authors: William G
authors: WG
authors: Stetler-Stevenson
mesh_terms: D000818:Animals; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D018827:Carcinoma, Lewis Lung / Q000098:blood supply / Q000178:diet therapy* / Q000473:pathology; D049109:Cell Proliferation; D004032:Diet; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007124:Immunoenzyme Techniques; D008055:Lipids / Q000737:chemistry*; D051379:Mice; D008810:Mice, Inbred C57BL; D009389:Neovascularization, Pathologic / Q000517:prevention & control*; D020411:Oligonucleotide Array Sequence Analysis; D010743:Phospholipids / Q000737:chemistry*; D008517:Phytotherapy; D028321:Plant Preparations / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D008055:Lipids; D010743:Phospholipids; D028321:Plant Preparations; D012333:RNA, Messenger