title: Polyphenol-rich extract of Nelumbo nucifera leaves inhibits alcohol-induced steatohepatitis via reducing hepatic lipid accumulation and anti-inflammation in C57BL/6J mice.
abstract: The present study was undertaken to evaluate the hepatoprotective effect mechanisms of Nelumbo nucifera leaves extract (NLE) in experimental alcoholic steatohepatitis animal models. We found that the NLE contained polyphenols (phenolic acids and flavonoids), and more than 70% of the main functional components in NLE could potentially provide benefits for alcoholic liver disease. The parameters of histopathology, immunohistochemistry, antioxidant defense, proinflammatory mediator and lipid synthesis-related proteins demonstrated the inhibitory effect of NLE on alcoholic steatohepatitis. Plasma and hepatic content analysis showed that NLE inhibited lipid accumulation by altering the levels of triglycerides (TG) and cholesterol (TC). Treatment with NLE increased the expression of the p-AMPK/AMPK ratio and PPAR-α. Furthermore, fatty acid oxidation and transport via carnitine palmitoyltransferase-1 (CPT1) and microsomal triglyceride transfer protein (MTP) were through the activation of the AMPK and PPAR-α signal. These results revealed that the polyphenol-rich component of NLE prevents alcoholic steatohepatitis by multiple pathways, including reduced lipid synthesis, enhanced fatty acid oxidation and transport responses, inhibited oxidative stress and facilitated anti-inflammation. Suggesting that NLE might be regarded as a beneficial food that has the potential to be developed as a natural agent for preventing alcoholic steatohepatitis.
authors: Institute of Biochemistry and Biotechnology, College of Medicine, Chung Shan Medical University, No. 110, Sec. 1, Jianguo North Road, Taichung 402, Taiwan. wcj@csmu.edu.tw.
authors: Chang-Chieh
authors: CC
authors: Tang
authors: Wea-Lung
authors: WL
authors: Lin
authors: Yi-Ju
authors: YJ
authors: Lee
authors: Yu-Chi
authors: YC
authors: Tang
authors: Chau-Jong
authors: CJ
authors: Wang
mesh_terms: D000438:Alcohols / Q000009:adverse effects; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D005234:Fatty Liver / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D050356:Lipid Metabolism / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D031653:Nelumbo / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage*; D018515:Plant Leaves / Q000737:chemistry; D059808:Polyphenols / Q000008:administration & dosage*
chemical_list: D000438:Alcohols; D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D059808:Polyphenols