title: Assays to examine endothelial cell migration, tube formation, and gene expression profiles.
abstract: Common methods for studying angiogenesis in vitro include the tube formation assay, the migration assay, and the study of the endothelial genome. The formation of capillary-like tubes in vitro on basement membrane matrix mimics many steps of the angiogenesis process in vivo and is used widely as a screening test for angiogenic or antiangiogenic factors. Other assays related to the study of angiogenesis include the cell migration assay, the study of gene expression changes during the process of angiogenesis, and the study of endothelial-derived microparticles. Protocols for these procedures will be described here.
authors: Neuroprotection Research Laboratory, Department of Radiology and Neurology, Harvard Medical School, Massachusetts General Hospital, Boston, MA, USA.
authors: Shuzhen
authors: S
authors: Guo
authors: Josephine
authors: J
authors: Lok
authors: Yi
authors: Y
authors: Liu
authors: Kazuhide
authors: K
authors: Hayakawa
authors: Wendy
authors: W
authors: Leung
authors: Changhong
authors: C
authors: Xing
authors: Xunming
authors: X
authors: Ji
authors: Eng H
authors: EH
authors: Lo
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D002465:Cell Movement; D002469:Cell Separation; D055252:Cell-Derived Microparticles / Q000502:physiology; D042783:Endothelial Cells / Q000502:physiology*; D004730:Endothelium, Vascular / Q000166:cytology; D020869:Gene Expression Profiling; D051379:Mice; D055806:Microvessels / Q000166:cytology; D018919:Neovascularization, Physiologic; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats; D059467:Transcriptome
chemical_list: D012333:RNA, Messenger