title: In vivo targeting of adult neural stem cells in the dentate gyrus by a split-cre approach.
abstract: We describe the labeling of adult neural stem cells (aNSCs) in the mouse and human dentate gyrus (DG) by the combinatorial expression of glial fibrillary acidic protein (GFAP) and Prominin1, as revealed by immunohistochemistry. Split-Cre-based genetic fate mapping of these double-positive cells in the adult murine DG reveals their NSC identity, as they are self-renewing and contribute to neurogenesis over several months. Their progeny reacts to stimuli such as voluntary exercise with increased neurogenesis. Prominin1+/GFAP+ cells also exist in the adult human DG, the only region in the human brain for which adult neurogenesis has been consistently reported. Our data, together with previous evidence of such double-positive NSCs in the developing murine brain and in neurogenic regions of vertebrates with widespread neurogenesis, suggest that Prominin1- and GFAP-expressing cells are NSCs in a wide range of species in development and adulthood.
authors: Institute of Stem Cell Research, Helmholtz Center Munich, Ingolstädter Landstrasse 1, 85764 Neuherberg/Munich, Germany ; Emil-Fischer Centre, Institute of Biochemistry, Friedrich-Alexander University Erlangen-Nuremberg, Fahrstrasse 17, 91054 Erlangen, Germany.
authors: Ruth
authors: R
authors: Beckervordersandforth
authors: Department of Physiological Genomics, Institute of Physiology, Ludwig-Maximilian University of Munich, Schillerstrasse 46, 80336 Munich, Germany.
authors: Aditi
authors: A
authors: Deshpande
authors: Emil-Fischer Centre, Institute of Biochemistry, Friedrich-Alexander University Erlangen-Nuremberg, Fahrstrasse 17, 91054 Erlangen, Germany ; Institute of Developmental Genetics, Helmholtz Center Munich, Ingolstädter Landstrasse 1, 85764 Neuherberg/Munich, Germany.
authors: Iris
authors: I
authors: Schäffner
authors: Department of Neurology, Friedrich-Alexander University Erlangen-Nuremberg, Schwabachanlage 6, 91054 Erlangen, Germany.
authors: Hagen B
authors: HB
authors: Huttner
authors: Department of Physiological Genomics, Institute of Physiology, Ludwig-Maximilian University of Munich, Schillerstrasse 46, 80336 Munich, Germany.
authors: Alexandra
authors: A
authors: Lepier
authors: Emil-Fischer Centre, Institute of Biochemistry, Friedrich-Alexander University Erlangen-Nuremberg, Fahrstrasse 17, 91054 Erlangen, Germany.
authors: Dieter Chichung
authors: DC
authors: Lie
authors: Institute of Stem Cell Research, Helmholtz Center Munich, Ingolstädter Landstrasse 1, 85764 Neuherberg/Munich, Germany ; Department of Physiological Genomics, Institute of Physiology, Ludwig-Maximilian University of Munich, Schillerstrasse 46, 80336 Munich, Germany ; Munich Cluster for Systems Neurology (SyNergy), 80336 Munich, Germany.
authors: Magdalena
authors: M
authors: Götz
mesh_terms: D000071916:AC133 Antigen; D053687:Adult Stem Cells / Q000378:metabolism*; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D018891:Dentate Gyrus / Q000166:cytology* / Q000378:metabolism*; D015870:Gene Expression; D018390:Gene Targeting; D017930:Genes, Reporter; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics / Q000378:metabolism; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D059765:Homologous Recombination; D006801:Humans; D051379:Mice; D058953:Neural Stem Cells / Q000378:metabolism*; D009457:Neuroglia / Q000378:metabolism; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D021381:Protein Transport
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D005904:Glial Fibrillary Acidic Protein; D006023:Glycoproteins; D010455:Peptides