title: The N-glycan profile of placental membrane glycoproteins alters during gestation and aging.
abstract: Alterations in the glycosylation of few membrane proteins from human placenta during gestation have been documented, but data on N-glycome of placental membrane proteins are still missing. The primary goal of this study was to obtain N-glycan profiles of human placental membrane proteins using a reliable, simple and high-throughput method. The second goal was to examine whether the N-glycan profile alters during gestation. Placental membrane proteins were isolated from women of different ages after first and third trimesters of pregnancy. The N-glycan fingerprint of membrane proteins was obtained using DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis (DSA-FACE). Lectin blotting was used to confirm DSA-FACE results. Observed gestation-related alterations were: greater abundance of core-fucosylated and multiantennary N-glycans, but lower amounts of bisected biantennary N-glycans together with a decrease in α2,3-sialylation. Age-related alterations were: more core Fuc and more α2,3-Sia in first trimester placentas from older women than in those from younger women; also less core Fuc and less α2,6-Sia in third trimester placentas from older women compared to those from younger women. This study represents the first N-glycan profiling of placental cell membrane proteins. These data represent a basis for future research on the N-glycome of placental proteins in different (patho)physiological conditions.
authors: INEP - Institute for the Application of Nuclear Energy, University of Belgrade, Belgrade, 381, Serbia. Electronic address: draganar@inep.co.rs.
authors: Dragana
authors: D
authors: Robajac
authors: INEP - Institute for the Application of Nuclear Energy, University of Belgrade, Belgrade, 381, Serbia.
authors: Romana
authors: R
authors: Masnikosa
authors: Department for Molecular Biomedical Research, VIB, Ghent, 32, Belgium; Department of Biomedical Molecular Biology, Ghent University, Ghent, 32, Belgium.
authors: Valerie
authors: V
authors: Vanhooren
authors: Department for Molecular Biomedical Research, VIB, Ghent, 32, Belgium; Department of Biomedical Molecular Biology, Ghent University, Ghent, 32, Belgium.
authors: Claude
authors: C
authors: Libert
authors: Clinic of Gynaecology and Obstetrics "Narodni Front", University of Belgrade, Belgrade, 381, Serbia.
authors: Željko
authors: Ž
authors: Miković
authors: INEP - Institute for the Application of Nuclear Energy, University of Belgrade, Belgrade, 381, Serbia.
authors: Olgica
authors: O
authors: Nedić
mesh_terms: D000328:Adult; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D005865:Gestational Age; D006801:Humans; D008423:Maternal Age; D008562:Membrane Glycoproteins / Q000032:analysis / Q000378:metabolism; D010920:Placenta / Q000378:metabolism*; D010929:Placentation / Q000502:physiology; D011134:Polysaccharides / Q000032:analysis / Q000378:metabolism; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000032:analysis / Q000378:metabolism; D011264:Pregnancy Trimesters / Q000502:physiology; D015203:Reproducibility of Results; D005644:alpha-L-Fucosidase / Q000378:metabolism / Q000494:pharmacology
chemical_list: D008562:Membrane Glycoproteins; D011134:Polysaccharides; D011257:Pregnancy Proteins; D005644:alpha-L-Fucosidase
keywords: Aging; Gestation; N-glycome; Placental membrane