title: Adipose tissue diacylglycerol acyltransferase activity and blood lipoprotein triglyceride enrichment in women with abdominal obesity.
abstract: Previous studies have suggested altered triglyceride (TG) storage in patients with abdominal obesity and blood lipid disorders.
  
  
  OBJECTIVE
  We hypothesized that women with abdominal obesity and a dysmetabolic profile have low DGAT activity in their abdominal fat compartments.
  
  
  METHODS
  Paired omental (OM) and subcutaneous (SC) adipose tissue samples were obtained surgically from 39 women undergoing abdominal hysterectomies. Body composition and fat distribution were measured by dual energy x-ray absorptiometry and computed tomography. DGAT activity was measured by acylation of sn-l,2-diacylglycerol with [(14)C] oleoyl-CoA in microsomal fractions isolated from whole adipose tissue homogenates. DGAT activity was calculated on the basis of picomoles (pmol) TG synthesized in the assay per min per mg lipid, per μg protein or per 1000 cells.
  
  
  RESULTS
  No depot differences were found when DGAT activity was reported per μg microsomal protein or per 1000 cells. DGAT activity in either depot was not associated with adipocyte diameters and blood lipid profile variables. DGAT activity per mg lipid was higher in OM than in abdominal SC adipose tissue (0.43 ± 0.20 vs. 0.34 ± 0.18 pmol/min/mg lipid, p < 0.05). OM DGAT activity was negatively correlated with OM adipocyte diameter and visceral adipose tissue area (r = -0.43, p < 0.01 and r = -0.38, p < 0.05 respectively). Plasma total, LDL and HDL TG levels were negatively associated with OM DGAT activity independent of total body fat mass (r = -0.39, p < 0.05, r = -0.46, p < 0.001 and r = -0.40, p < 0.05 respectively).
  
  
  CONCLUSIONS
  A defect in adipose tissue DGAT activity is predictive of adiposity and blood lipoprotein TG enrichment only when considering activity per tissue lipid mass.
authors: Endocrinology and Nephrology, Laval University Medical Research Center, Quebec, Canada; Department of Nutrition, Laval University, Quebec, Canada.
authors: Julie Anne
authors: JA
authors: Côté
authors: Endocrinology and Nephrology, Laval University Medical Research Center, Quebec, Canada; Department of Nutrition, Laval University, Quebec, Canada.
authors: Mélanie
authors: M
authors: Nadeau
authors: Gynecology Unit, Laval University Medical Research Center, Quebec, Canada.
authors: Mathieu
authors: M
authors: Leboeuf
authors: Gynecology Unit, Laval University Medical Research Center, Quebec, Canada.
authors: Line
authors: L
authors: Blackburn
authors: Endocrinology and Nephrology, Laval University Medical Research Center, Quebec, Canada; Department of Nutrition, Laval University, Quebec, Canada. Electronic address: andre.tchernof@crchul.ulaval.ca.
authors: André
authors: A
authors: Tchernof
mesh_terms: D000328:Adult; D051048:Diacylglycerol O-Acyltransferase / Q000378:metabolism*; D005260:Female; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood; D008875:Middle Aged; D056128:Obesity, Abdominal / Q000201:enzymology*; D009852:Omentum; D050177:Overweight / Q000378:metabolism; D050151:Subcutaneous Fat / Q000378:metabolism; D050507:Subcutaneous Fat, Abdominal / Q000378:metabolism*; D014280:Triglycerides / Q000097:blood*
chemical_list: C034396:HDL-triglyceride; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D014280:Triglycerides; C030087:lipoprotein triglyceride; D051048:Diacylglycerol O-Acyltransferase
keywords: Computed tomography; Omental adipocytes; Visceral fat; Women