title: Selection of rodent species appropriate for mtDNA transfer to generate transmitochondrial mito-mice expressing mitochondrial respiration defects.
abstract: Previous reports have shown that transmitochondrial mito-mice with nuclear DNA from Mus musculus and mtDNA from M. spretus do not express respiration defects, whereas those with mtDNA from Rattus norvegicus cannot be generated from ES cybrids with mtDNA from R. norvegicus due to inducing significant respiration defects and resultant losing multipotency. Here, we isolated transmitochondrial cybrids with mtDNA from various rodent species classified between M. spretus and R. norvegicus, and compared the O2 consumption rates. The results showed a strong negative correlation between phylogenetic distance and reduction of O2 consumption rates, which would be due to the coevolution of nuclear and mitochondrial genomes and the resultant incompatibility between the nuclear genome from M. musculus and the mitochondrial genome from the other rodent species. These observations suggested that M. caroli was an appropriate mtDNA donor to generate transmitochondrial mito-mice with nuclear DNA from M. musculus. Then, we generated ES cybrids with M. caroli mtDNA, and found that these ES cybrids expressed respiration defects without losing multipotency and can be used to generate transmitochondrial mito-mice expressing mitochondrial disorders.
authors: Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan.
authors: Shunkei
authors: S
authors: Enoki
authors: Akinori
authors: A
authors: Shimizu
authors: Chisato
authors: C
authors: Hayashi
authors: Hirotake
authors: H
authors: Imanishi
authors: Osamu
authors: O
authors: Hashizume
authors: Kazuyuki
authors: K
authors: Mekada
authors: Hitoshi
authors: H
authors: Suzuki
authors: Tetsuo
authors: T
authors: Hashimoto
authors: Kazuto
authors: K
authors: Nakada
authors: Jun-Ichi
authors: J
authors: Hayashi
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000378:metabolism; D053595:Embryonic Stem Cells; D019143:Evolution, Molecular; D022761:Gene Transfer, Horizontal / Q000235:genetics*; D051379:Mice / Q000235:genetics*; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D028361:Mitochondrial Diseases / Q000235:genetics*; D009154:Mutation; D010101:Oxygen Consumption; D010802:Phylogeny; D051381:Rats / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial