title: Reformatting of scFv antibodies into the scFv-Fc format and their downstream purification.
abstract: The scFv-Fc format allows for rapid characterization of candidate scFvs isolated from phage display libraries before conversion into a full-length IgG. This format offers several advantages over the phage display-derived scFv, including bivalent binding, longer half-life, and Fc-mediated effector functions. Here, a detailed method is presented, which describes the cloning, expression, and purification of an scFv-Fc fragment, starting from scFv fragments obtained from a phage display library. This method facilitates the rapid screening of candidate antibodies, prior to a more time-consuming conversion into a full IgG format. Alternatively, the scFv-Fc format may be used in the clinic for therapeutic applications.
authors: Philochem AG, Zurich, Switzerland.
authors: Emil
authors: E
authors: Bujak
authors: Mattia
authors: M
authors: Matasci
authors: Dario
authors: D
authors: Neri
authors: Sarah
authors: S
authors: Wulhfard
mesh_terms: D007141:Immunoglobulin Fc Fragments / Q000235:genetics / Q000378:metabolism*; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000378:metabolism; D019151:Peptide Library; D057127:Single-Chain Antibodies / Q000235:genetics / Q000378:metabolism*
chemical_list: D007141:Immunoglobulin Fc Fragments; D007135:Immunoglobulin Variable Region; D019151:Peptide Library; D057127:Single-Chain Antibodies