title: Multiplex cell and lineage tracking with combinatorial labels.
abstract: We present a method to label and trace the lineage of multiple neural progenitors simultaneously in vertebrate animals via multiaddressable genome-integrative color (MAGIC) markers. We achieve permanent expression of combinatorial labels from new Brainbow transgenes introduced in embryonic neural progenitors with electroporation of transposon vectors. In the mouse forebrain and chicken spinal cord, this approach allows us to track neural progenitor's descent during pre- and postnatal neurogenesis or perinatal gliogenesis in long-term experiments. Color labels delineate cytoarchitecture, resolve spatially intermixed clones, and specify the lineage of astroglial subtypes and adult neural stem cells. Combining colors and subcellular locations provides an expanded marker palette to individualize clones. We show that this approach is also applicable to modulate specific signaling pathways in a mosaic manner while color-coding the status of individual cells regarding induced molecular perturbations. This method opens new avenues for clonal and functional analysis in varied experimental models and contexts.
authors: INSERM, U968, Paris 75012, France; Sorbonne Universités, UPMC Univ Paris 06, UMR_S 968, Institut de la Vision, Paris 75012, France; CNRS, UMR 7210, Paris 75012, France.
authors: Karine
authors: K
authors: Loulier
authors: INSERM, U968, Paris 75012, France; Sorbonne Universités, UPMC Univ Paris 06, UMR_S 968, Institut de la Vision, Paris 75012, France; CNRS, UMR 7210, Paris 75012, France.
authors: Raphaëlle
authors: R
authors: Barry
authors: Laboratoire d'Optique et Biosciences, Ecole Polytechnique, Palaiseau 91128, France; CNRS, UMR 7645, Palaiseau 91128, France; INSERM, U696, Palaiseau 91128, France.
authors: Pierre
authors: P
authors: Mahou
authors: INSERM, U968, Paris 75012, France; Sorbonne Universités, UPMC Univ Paris 06, UMR_S 968, Institut de la Vision, Paris 75012, France; CNRS, UMR 7210, Paris 75012, France.
authors: Yann
authors: Y
authors: Le Franc
authors: Laboratoire d'Optique et Biosciences, Ecole Polytechnique, Palaiseau 91128, France; CNRS, UMR 7645, Palaiseau 91128, France; INSERM, U696, Palaiseau 91128, France.
authors: Willy
authors: W
authors: Supatto
authors: INSERM, U968, Paris 75012, France; Sorbonne Universités, UPMC Univ Paris 06, UMR_S 968, Institut de la Vision, Paris 75012, France; CNRS, UMR 7210, Paris 75012, France.
authors: Katherine S
authors: KS
authors: Matho
authors: INSERM, U968, Paris 75012, France; Sorbonne Universités, UPMC Univ Paris 06, UMR_S 968, Institut de la Vision, Paris 75012, France; CNRS, UMR 7210, Paris 75012, France.
authors: Siohoi
authors: S
authors: Ieng
authors: INSERM, U968, Paris 75012, France; Sorbonne Universités, UPMC Univ Paris 06, UMR_S 968, Institut de la Vision, Paris 75012, France; CNRS, UMR 7210, Paris 75012, France.
authors: Stéphane
authors: S
authors: Fouquet
authors: INSERM, U968, Paris 75012, France; Sorbonne Universités, UPMC Univ Paris 06, UMR_S 968, Institut de la Vision, Paris 75012, France; CNRS, UMR 7210, Paris 75012, France.
authors: Elisabeth
authors: E
authors: Dupin
authors: INSERM, U968, Paris 75012, France; Sorbonne Universités, UPMC Univ Paris 06, UMR_S 968, Institut de la Vision, Paris 75012, France; CNRS, UMR 7210, Paris 75012, France.
authors: Ryad
authors: R
authors: Benosman
authors: INSERM, U968, Paris 75012, France; Sorbonne Universités, UPMC Univ Paris 06, UMR_S 968, Institut de la Vision, Paris 75012, France; CNRS, UMR 7210, Paris 75012, France.
authors: Alain
authors: A
authors: Chédotal
authors: Laboratoire d'Optique et Biosciences, Ecole Polytechnique, Palaiseau 91128, France; CNRS, UMR 7645, Palaiseau 91128, France; INSERM, U696, Palaiseau 91128, France.
authors: Emmanuel
authors: E
authors: Beaurepaire
authors: Ecole Normale Supérieure, Institut de Biologie de l'ENS, IBENS, Paris 75005, France; INSERM, U1024, Paris 75005, France; CNRS, UMR 8197, Paris 75005, France. Electronic address: xavier.morin@ens.fr.
authors: Xavier
authors: X
authors: Morin
authors: INSERM, U968, Paris 75012, France; Sorbonne Universités, UPMC Univ Paris 06, UMR_S 968, Institut de la Vision, Paris 75012, France; CNRS, UMR 7210, Paris 75012, France. Electronic address: jean.livet@inserm.fr.
authors: Jean
authors: J
authors: Livet
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000166:cytology* / Q000196:embryology / Q000254:growth & development; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology; D002642:Chick Embryo; D003124:Colorimetry; D018274:Electroporation; D004622:Embryo, Mammalian; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D055495:Neurogenesis / Q000502:physiology; D009457:Neuroglia / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D013116:Spinal Cord / Q000166:cytology* / Q000196:embryology; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*; D013997:Time Factors; D019895:Transposases / Q000502:physiology
chemical_list: D008164:Luminescent Proteins; D019895:Transposases