title: Real-time PCR assay for rapid detection of epidemiologically and clinically significant Mycobacterium tuberculosis Beijing genotype isolates.
abstract: Mycobacterium tuberculosis Beijing genotype strains are rapidly disseminating, frequently hypervirulent, and multidrug resistant. Here, we describe a method for their rapid detection by real-time PCR that targets the specific IS6110 insertion in the dnaA-dnaN genome region. The method was evaluated with a geographically and genetically diverse collection representing areas in East Asia and the former Soviet Union in which the Beijing genotype is endemic and epidemic (i.e., major foci of its global propagation) and with clinical specimens.
authors: Laboratory of Molecular Microbiology, St. Petersburg Pasteur Institute, St. Petersburg, Russia.
authors: Igor
authors: I
authors: Mokrousov
authors: Anna
authors: A
authors: Vyazovaya
authors: Viacheslav
authors: V
authors: Zhuravlev
authors: Tatiana
authors: T
authors: Otten
authors: Julie
authors: J
authors: Millet
authors: Wei-Wei
authors: WW
authors: Jiao
authors: A-Dong
authors: AD
authors: Shen
authors: Nalin
authors: N
authors: Rastogi
authors: Boris
authors: B
authors: Vishnevsky
authors: Olga
authors: O
authors: Narvskaya
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D005838:Genotype; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D014376:Tuberculosis / Q000175:diagnosis*
chemical_list: D004269:DNA, Bacterial