title: [Association of IL-10 promoter polymorphism and plasma levels with susceptibility to vocal cords leukoplakia].
abstract: OBJECTIVE
  To investigated the association between interleukin-10(IL-10)-1082/-819/-592 promoter polymorphism, plasma IL-10 levels and risk of vocal leukoplakia.
  
  
  METHODS
  A case-control study of 61 patients with vocal cords leukoplakia and 119 healthy subjects were performed. Genotypes for the IL-10 gene (IL-10 -1082 G/A, -819 C/T and -592 C/A) were determined using pyrosequencing and plasma IL-10 levels were analyzed by ELISA.
  
  
  RESULTS
  Smoking and alcohol consumption increased the risk of vocal leukoplakia (OR = 4.73, 95%CI:2.4-9.1;OR = 5.70, 95%CI:2.9-11.2, P < 0.01) . Patients with vocal cord leukoplakia had significantly higher frequencies of AC at -592 and -819 (OR = 1.93, 95%CI:0.97-3.81, P = 0.05) and AG at -1082(OR = 2.14, 95%CI:0.87-5.27, P = 0.092) than control. Vocal leukoplakia patients had higher concentration of plasma IL-10 (21.6 ± 0.5) pg/ml (X(-) ± s) than controls (19.0 ± 1.1)pg/ml(t = 2.08, P = 0.043) and the haplotype containing the G allele was associated with higher plasma IL-10 concentrations (24.3 ± 5.7) pg/ml compared with the ATA haplotype(19.9 ± 4.7) pg/ml (t = -2.64, P = 0.008).
  
  
  CONCLUSIONS
  IL-10-1082/-819/-592 promoter polymorphism and high concentration of plasma IL-10 are associated with vocal cord leukoplakia, and the concentration of plasma IL-10 is associated with the genotypes of IL-10 promoter polymorphism.
authors: Department of Otorhinolaryngology Head and Neck Surgery, Eye, Ear, Nose and Throat Hospital of Fudan University, Shanghai 200031, China.
authors: Jian
authors: J
authors: Zhou
authors: Lei
authors: L
authors: Tao
authors: Duo
authors: D
authors: Zhang
authors: Bin
authors: B
authors: Chen
authors: Wei-jing
authors: WJ
authors: Tang
authors: Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China. Email: lulunew2003@163.com.
authors: Li-ming
authors: LM
authors: Lu
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D016753:Interleukin-10 / Q000097:blood / Q000235:genetics*; D007971:Leukoplakia / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D014827:Vocal Cords / Q000473:pathology*
chemical_list: C508609:IL10 protein, human; D016753:Interleukin-10