title: Methodologies for measurement of cardiac markers.
abstract: Antibody-based tests are the primary technology used for clinical measurement of cardiac biomarkers in peripheral circulation. This article focuses on the principles of immunometric methods that have been applied to cardiac biomarkers of widespread clinical utility (CKMB, Troponins, and B-type natriuretic proteins) and of more recent clinical testing (ST2, Galectin-2, and myosin binding protein C). How these principles are applied in the design of immunometric assays and how they influence assay performance in quantifying cardiac biomarkers in biologic fluids (serum or plasma) is discussed.
authors: Pathology Service, Clinical Pathology Core Laboratories (Special Chemistry), Pathology, Massachusetts General Hospital, Harvard Medical School, GRB554, 55 Fruit Street, Boston, MA 02114, USA. Electronic address: sluss.patrick@mgh.harvard.edu.
authors: Patrick M
authors: PM
authors: Sluss
mesh_terms: D015415:Biomarkers / Q000097:blood*; D002352:Carrier Proteins / Q000097:blood; D003402:Creatine Kinase / Q000097:blood; D003935:Diagnostic Techniques, Cardiovascular; D037502:Galectin 3 / Q000097:blood; D006331:Heart Diseases / Q000175:diagnosis*; D006801:Humans; D000072179:Interleukin-1 Receptor-Like 1 Protein; D008955:Models, Cardiovascular; D020097:Natriuretic Peptide, Brain / Q000097:blood; D019095:Point-of-Care Systems; D011956:Receptors, Cell Surface / Q000097:blood; D014336:Troponin / Q000097:blood; D000099163:Myosin Binding Protein C
chemical_list: D015415:Biomarkers; D002352:Carrier Proteins; D003402:Creatine Kinase; D037502:Galectin 3; D000072179:Interleukin-1 Receptor-Like 1 Protein; D020097:Natriuretic Peptide, Brain; D011956:Receptors, Cell Surface; D014336:Troponin; D000099163:Myosin Binding Protein C; C079241:IL1RL1 protein, human
keywords: Assay design; Assay principles; Biomarkers; CKMB; Cardiac markers; Immunoassay; Laboratory medicine; Troponins