title: A Mutation inMtap2Is Associated with Arrest of Mammalian Spermatocytes before the First Meiotic Division.
abstract: In spite of evolutionary conservation of meiosis, many of the genes that control mammalian meiosis are still unknown. We report here that the ENU-inducedrepro4mutation, identified in a screen to uncover genes that control mouse meiosis, causes failure of spermatocytes to exit meiotic prophase I via the G2/MI transition. Major events of meiotic prophase I occurred normally in affected spermatocytes and known regulators of the meiotic G2/MI transition were present and functional. Deep sequencing of mutant DNA revealed a mutation located in an intron ofMtap2gene, encoding microtubule-associated protein 2, and levels ofMtap2transcript were reduced in mutant testes. This evidence implicates MTAP2 as required directly or indirectly for completion of meiosis and normal spermatogenesis in mammals.
authors: The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA.
authors: Fengyun
authors: F
authors: Sun
authors: The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA.
authors: Mary Ann
authors: MA
authors: Handel
keywords: meiosis; microtubule-associated protein; spermatogenesis