title: Influenza virus vaccine expressing fusion and attachment protein epitopes of respiratory syncytial virus induces protective antibodies in BALB/c mice.
abstract: Respiratory syncytial virus (RSV) is an important viral pathogen that causes life-threatening respiratory infections in both infants and the elderly; no vaccines are at present available. In this report, we examined the use of influenza virus as a vehicle for production of an experimental RSV vaccine. We used reverse genetics to generate a recombinant influenza A virus with epitopes from the RSV fusion (F) and attachment (G) proteins (rFlu/RSV/F+G) in the influenza virus nonstructural (NS1) protein gene. Expression of RSV F+G epitope proteins was confirmed by Western blotting, and no changes in viral morphology were evident following examination by electron microscopy. BALB/c mice immunized intranasally with rFlu/RSV/F+G showed viral-specific antibody responses against both influenza and RSV. Total IgG, IgG1, IgG2a and IgA were measured in mice immunized with rFlu/RSV/F+G, revealing robust cellular and mucosal immune responses. Furthermore, we found that rFlu/RSV/F+G conferred protection against subsequent influenza and RSV challenges, showing significant decreases in viral replication and obvious attenuation of histopathological changes associated with viral infections. These findings suggest that rFlu/RSV/F+G is a promising vaccine candidate, which should be further assessed using cotton rat and primate models.
authors: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China; Department of Pathogenic Biology and Medical Immunology, School of Basic Medical Sciences, Ningxia Medical University, Yinchuan 750004, China; Center of Clinical Laboratory, 302 Military Hospital, Beijing 100039, China.
authors: Chengrong
authors: C
authors: Bian
authors: National Institutes for Food and Drug Control, Beijing 100050, China.
authors: Shuzhen
authors: S
authors: Liu
authors: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China; Department of Pathogenic Biology and Medical Immunology, School of Basic Medical Sciences, Ningxia Medical University, Yinchuan 750004, China.
authors: Na
authors: N
authors: Liu
authors: Laboratory Animal Center of the Academy of Military and Medical Sciences, Beijing 100071, China.
authors: Guangzhou
authors: G
authors: Zhang
authors: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China.
authors: Li
authors: L
authors: Xing
authors: Department of Blood Transfusion, Chinese PLA General Hospital, Beijing 100853, China.
authors: Yingwei
authors: Y
authors: Song
authors: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China.
authors: Yueqiang
authors: Y
authors: Duan
authors: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China.
authors: Hongjing
authors: H
authors: Gu
authors: Department of Pathogenic Biology and Medical Immunology, School of Basic Medical Sciences, Ningxia Medical University, Yinchuan 750004, China.
authors: Ya
authors: Y
authors: Zhou
authors: Department of Hepatobiliary, 302 Military Hospital, Beijing 100039, China.
authors: Peirui
authors: P
authors: Zhang
authors: Department of Hepatobiliary, 302 Military Hospital, Beijing 100039, China.
authors: Zhiwei
authors: Z
authors: Li
authors: Department of Hepatobiliary, 302 Military Hospital, Beijing 100039, China.
authors: Keming
authors: K
authors: Zhang
authors: Department of Hepatobiliary, 302 Military Hospital, Beijing 100039, China.
authors: Zhaohai
authors: Z
authors: Wang
authors: Department of Hepatobiliary, 302 Military Hospital, Beijing 100039, China. Electronic address: zhangsg302@hotmail.com.
authors: Shaogeng
authors: S
authors: Zhang
authors: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China. Electronic address: xiliangw@126.com.
authors: Xiliang
authors: X
authors: Wang
authors: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China; Department of Hepatobiliary, 302 Military Hospital, Beijing 100039, China. Electronic address: ypenghuiamms@hotmail.com.
authors: Penghui
authors: P
authors: Yang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D002460:Cell Line; D002522:Chlorocebus aethiops; D004195:Disease Models, Animal; D004285:Dogs; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D005260:Female; D023061:Gene Order; D005822:Genetic Vectors / Q000235:genetics; D018928:Immunity, Mucosal; D007114:Immunization; D007252:Influenza Vaccines / Q000235:genetics / Q000276:immunology*; D008099:Liver / Q000276:immunology / Q000821:virology; D008168:Lung / Q000276:immunology / Q000473:pathology / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology* / Q000517:prevention & control*; D012136:Respiratory Syncytial Viruses / Q000235:genetics / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D014760:Viral Fusion Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000939:Epitopes; D007252:Influenza Vaccines; D014760:Viral Fusion Proteins
keywords: Fusion and attachment protein epitopes; Influenza virus; RSV; Viral vector