title: Reconstitution of a 10-gene pathway for synthesis of the plant alkaloid dihydrosanguinarine in Saccharomyces cerevisiae.
abstract: Benzylisoquinoline alkaloids (BIAs) represent a large class of plant secondary metabolites, including pharmaceuticals such as morphine, codeine and their derivatives. Large-scale production of BIA-based pharmaceuticals is limited to extraction and derivatization of alkaloids that accumulate in planta. Synthesis of BIAs in microbial hosts could bypass such limitations and transform both industrial production of BIAs with recognized value and research into uncharacterized BIAs. Here we reconstitute a 10-gene plant pathway in Saccharomyces cerevisiae that allows for the production of dihydrosanguinarine and its oxidized derivative sanguinarine from (R,S)-norlaudanosoline. Synthesis of dihydrosanguinarine also yields the side-products N-methylscoulerine and N-methylcheilanthifoline, the latter of which has not been detected in plants. This work represents the longest reconstituted alkaloid pathway ever assembled in yeast and demonstrates the feasibility of the production of high-value alkaloids in microbial systems.
authors: 1] Department of Biology, Concordia University, Montréal, Québec, Canada H4B 1R6 [2] Centre for Structural and Functional Genomics, Concordia University, Montréal, Québec, Canada H4B 1R6 [3].
authors: Elena
authors: E
authors: Fossati
authors: 1] Department of Biology, Concordia University, Montréal, Québec, Canada H4B 1R6 [2] Centre for Structural and Functional Genomics, Concordia University, Montréal, Québec, Canada H4B 1R6 [3].
authors: Andrew
authors: A
authors: Ekins
authors: 1] Department of Biology, Concordia University, Montréal, Québec, Canada H4B 1R6 [2] Centre for Structural and Functional Genomics, Concordia University, Montréal, Québec, Canada H4B 1R6 [3].
authors: Lauren
authors: L
authors: Narcross
authors: 1] Department of Biology, Concordia University, Montréal, Québec, Canada H4B 1R6 [2] Centre for Structural and Functional Genomics, Concordia University, Montréal, Québec, Canada H4B 1R6.
authors: Yun
authors: Y
authors: Zhu
authors: Centre for Structural and Functional Genomics, Concordia University, Montréal, Québec, Canada H4B 1R6.
authors: Jean-Pierre
authors: JP
authors: Falgueyret
authors: Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada T2N 1N4.
authors: Guillaume A W
authors: GA
authors: Beaudoin
authors: Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada T2N 1N4.
authors: Peter J
authors: PJ
authors: Facchini
authors: 1] Department of Biology, Concordia University, Montréal, Québec, Canada H4B 1R6 [2] Centre for Structural and Functional Genomics, Concordia University, Montréal, Québec, Canada H4B 1R6.
authors: Vincent J J
authors: VJ
authors: Martin
mesh_terms: D000470:Alkaloids / Q000096:biosynthesis; D053119:Benzophenanthridines / Q000096:biosynthesis*; D017343:Genes, Plant; D005822:Genetic Vectors; D007546:Isoquinolines; D010207:Papaver / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D013765:Tetrahydropapaveroline / Q000378:metabolism*; D014170:Transformation, Genetic / Q000235:genetics*
chemical_list: D000470:Alkaloids; D053119:Benzophenanthridines; D007546:Isoquinolines; C501843:dihydrosanguinarine; D013765:Tetrahydropapaveroline