title: Dynamic compression and co-culture with nucleus pulposus cells promotes proliferation and differentiation of adipose-derived mesenchymal stem cells.
abstract: Adipose-derived stem cells (ASCs) are a set of multi potent stem cells potentially used in cartilage tissue engineering. We hypothesized that the effect of dynamic compression and co-culture with nucleus pulposus cells (NPCs) promotes ASC proliferation and chondrogenic differentiation. A controlled dynamic compression loading device was utilized to stimulate ASCs obtained from Sprague Dawley (SD) rats and identified by flow cytometry. The proliferation index was measured by carboxyfluorescein succinimidyl ester (CFSE) staining. Dynamic compression, as well as co-culture enhanced chondrogenic differentiation of ASCs as indicated by the expression of SOX-9, type-II collagen and aggrecan, which were measured by real-time PCR and Western blot. In our study, we found dynamic compression promoted the proliferation of ASCs and induced its differentiation into NP-like cells. Combination of dynamic compression and co-culture showed an additive effect on NP-like cell differentiation.
authors: The Affiliated Hospital of Nanjing University, Nanjing Drum Tower Hospital of Nanjing, China.
authors: Jun
authors: J
authors: Dai
authors: Department of Orthopedic, Tongji Hospital of Tongji Medical College of HUST, Wuhan, China.
authors: Huan
authors: H
authors: Wang
authors: Department of Orthopedic, Tongji Hospital of Tongji Medical College of HUST, Wuhan, China.
authors: Guo
authors: G
authors: Liu
authors: Department of Orthopedic, Tongji Hospital of Tongji Medical College of HUST, Wuhan, China.
authors: Zhanjiang
authors: Z
authors: Xu
authors: Department of Orthopedic, Tongji Hospital of Tongji Medical College of HUST, Wuhan, China.
authors: Feng
authors: F
authors: Li
authors: Department of Orthopedic, Tongji Hospital of Tongji Medical College of HUST, Wuhan, China. Electronic address: fanghuangtjh@126.com.
authors: Huang
authors: H
authors: Fang
mesh_terms: D053674:Aggrecans / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D020219:Chondrogenesis; D018920:Coculture Techniques; D024043:Collagen Type II / Q000378:metabolism; D007403:Intervertebral Disc / Q000166:cytology / Q000502:physiology; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D013314:Stress, Mechanical; D023822:Tissue Engineering
chemical_list: D053674:Aggrecans; D024043:Collagen Type II
keywords: Adipose-derived mesenchymal stem cells; Co-culture; Dynamic compression; Intervertebral disc degeneration; Nucleus pulposus cells