title: Harnessing G-tetrad scaffolds within G-quadruplex forming aptamers for fluorescence detection strategies.
abstract: G-tetrads are essential structural components required for the formation of G-quadruplexes. Replacement of G nucleobases within G-tetrads with fluorescent 8-aryl-dG residues provides diagnostic handles that are universally applicable to antiparallel G-quadruplex aptamers, as they can stabilize quadruplex folding and maintain aptamer function.
authors: Departments of Chemistry & Toxicology, University of Guelph, Guelph, N1G 2W1, Ontario, Canada. rmanderv@uoguelph.ca.
authors: Michael
authors: M
authors: Sproviero
authors: Richard A
authors: RA
authors: Manderville
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D001483:Base Sequence; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D054856:G-Quadruplexes; D008958:Models, Molecular; D011485:Protein Binding; D013917:Thrombin / Q000032:analysis / Q000378:metabolism
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; C081676:thrombin aptamer; D013917:Thrombin