title: Analysis of community dynamics in environmental samples using denaturing gradient gel electrophoresis.
abstract: Denaturing gradient gel electrophoresis (DGGE) is a culture-independent fingerprinting technique that allows for rapid comparative analysis of changes to microbial communities. 16S rRNA genes amplified from environmental samples can be separated based on their melting behavior in a denaturing gradient of urea and formamide. A fingerprint of the microbial community is generated with each band on the gel assumed to correspond to a different bacterial species. Community dynamics can then be assessed through statistical analysis of DGGE profiles and the sequencing of excised bands.
authors: Department of Biogeochemistry, Max-Planck-Institute for Terrestrial Microbiology, Marburg, Germany.
authors: Claire L
authors: CL
authors: Thompson
mesh_terms: D058645:Denaturing Gradient Gel Electrophoresis / Q000379:methods; D004783:Environmental Microbiology; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA