title: DNA damage induced by carbon ions (C³⁺) beam accessed by independent component analysis of infrared spectra.
abstract: OBJECTIVE
  To understand the degradation processes and role of secondary species on deoxyribonucleic acid (DNA)-damaged fragments as a result of irradiation with energetic ions.
  
  
  METHODS
  Damage caused to DNA thin films as a result of exposure to 4 keV carbon ions beam was accessed by analyzing the infrared spectra, obtained in situ for different irradiation times, with both bi-dimensional (2D) correlation spectroscopy and independent component analysis (ICA).
  
  
  RESULTS
  Results indicated that deoxyribose, phosphate and base groups of the DNA molecule were being damaged and new reaction products as oxime and furfural groups are being formed.
  
  
  CONCLUSIONS
  Damage on DNA bases is consistent with the formation of oxime products which react with DNA deoxyribose products forming furfural groups and confirming that DNA damage is caused by direct and indirect processes.
authors: CEFITEC, Departamento de Física, Faculdade de Ciências e Tecnologia, FCT, Universidade Nova de Lisboa , Caparica , Portugal.
authors: Maria
authors: M
authors: Raposo
authors: Margarida
authors: M
authors: Coelho
authors: Paulo J
authors: PJ
authors: Gomes
authors: Pedro
authors: P
authors: Vieira
authors: Paulo A
authors: PA
authors: Ribeiro
authors: Nigel J
authors: NJ
authors: Mason
authors: Christopher A
authors: CA
authors: Hunniford
authors: Robert W
authors: RW
authors: McCullough
mesh_terms: D000818:Animals; D002244:Carbon / Q000009:adverse effects*; D002417:Cattle; D004249:DNA Damage; D013055:Spectrophotometry, Infrared; D013223:Statistics as Topic / Q000379:methods*
chemical_list: D002244:Carbon