title: Toxicity study of cerium oxide nanoparticles in human neuroblastoma cells.
abstract: The present study consisted of cytotoxic, genotoxic, and oxidative stress responses of human neuroblastoma cell line (IMR32) following exposure to different doses of cerium oxide nanoparticles (CeO2 NPs; nanoceria) and its microparticles (MPs) for 24 hours. Cytotoxicity was evaluated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide and lactate dehydrogenase assays whereas genotoxicity was assessed using the cytokinesis-block micronucleus and comet assays. A battery of assays including lipid peroxidation, reactive oxygen species (ROS), hydrogen peroxide, reduced glutathione, nitric oxide, glutathione reductase, glutathione peroxidase, superoxide dismutase, catalase, and glutathione S-transferase were performed to test the hypothesis that ROS was responsible for the toxicity of nanoceria. The results showed that nanosized CeO2 was more toxic than cerium oxide MPs. Hence, further study on safety evaluation of CeO2 NPs on other models is recommended.
authors: Toxicology Unit, Biology Division, Indian Institute of Chemical Technology, Hyderabad, Andhra Pradesh 500 007, India. Email: paramgrover@gmail.com; grover@iict.res.in.
authors: Monika
authors: M
authors: Kumari
authors: Shailendra Pratap
authors: SP
authors: Singh
authors: Srinivas
authors: S
authors: Chinde
authors: Mohammed Fazlur
authors: MF
authors: Rahman
authors: Mohammed
authors: M
authors: Mahboob
authors: Paramjit
authors: P
authors: Grover
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D045744:Cell Line, Tumor; D002563:Cerium / Q000633:toxicity*; D016207:Cytokines / Q000378:metabolism; D004260:DNA Repair / Q000187:drug effects; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D053758:Nanoparticles / Q000633:toxicity*; D009447:Neuroblastoma / Q000473:pathology*; D018384:Oxidative Stress / Q000187:drug effects; D010316:Particle Size
chemical_list: D000975:Antioxidants; D016207:Cytokines; D002563:Cerium; C030583:ceric oxide; D007770:L-Lactate Dehydrogenase
keywords: cerium oxide nanoparticles; cytotoxicity; genotoxicity; human neuroblastoma cells; oxidative stress