title: miR-335 represents an independent prognostic marker in epithelial ovarian cancer.
abstract: OBJECTIVE
  To investigate the clinical significance of miR-335 expression in epithelial ovarian cancer (EOC).
  
  
  METHODS
  Expression of miR-335 in normal ovaries, EOC, and omental metastases was determined by quantitative realtime polymerase chain reaction. Overall survival (OS) and relapse-free survival (RFS) were examined using KaplanMeier curves and the Cox proportional hazards regression model.
  
  
  RESULTS
  miR-335 expression level was reduced in malignant tissue samples, especially in omental metastases. Low miR-335 levels in EOC were associated with shorter OS (P < .001) and RFS (P = .002). In the multivariate analyses, low miR-335 levels emerged as an independent prognostic factor for poor OS (P = .005) and RFS (P = .016). miR-335 was downregulated in the recurrence group compared with the nonrecurrence group. The area under the receiver operating characteristic curve was 0.818 (95% confidence interval, 0.675-0.961).
  
  
  CONCLUSIONS
  Our results indicate that miR-335 relates to the prognosis of patients with EOC and is a promising predictor of EOC recurrence.
authors: Dept of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan. Hubei Province, No. 1277, Jiefang Avenue, Wuhan 443000, China; zehuawang@163.net.
authors: Jin
authors: J
authors: Cao
authors: Jing
authors: J
authors: Cai
authors: Da
authors: D
authors: Huang
authors: Qing
authors: Q
authors: Han
authors: Yanxia
authors: Y
authors: Chen
authors: Qiang
authors: Q
authors: Yang
authors: Chun
authors: C
authors: Yang
authors: Yan
authors: Y
authors: Kuang
authors: Donglin
authors: D
authors: Li
authors: Zehua
authors: Z
authors: Wang
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D002277:Carcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D011379:Prognosis; D012307:Risk Factors
chemical_list: D014408:Biomarkers, Tumor; C556347:MIRN335 microRNA, human; D035683:MicroRNAs
keywords: EOC; Metastasis; MicroRNA; Recurrence; Survival