title: Calcium mediated formation of phosphorylcholine-based polyplexes for efficient knockdown of epidermal growth factor receptors (EGFR) in HeLa cells.
abstract: 2-Methacryloxyethyl phosphorylcholine (MPC) materials are well studied due to their excellent biocompatibility and are currently being used in many clinical applications. In this study, MPC based homopolymers and copolymers are prepared and are subsequently evaluated for their charge inversion properties in the presence of cations and subsequent DNA binding efficacies. These polymers are then studied for their epidermal growth factor receptor (EGFR) specific siRNA delivery in HeLa cells. The homopolymers of MPC and their copolymers show efficient EGFR knockdown efficacies in HeLa cells both in the presence and absence of serum proteins.
authors: Department of Chemical and Materials Engineering, University of Alberta, 116 St and 85 Ave, Edmonton, AB, Canada. narain@ualberta.ca.
authors: Marya
authors: M
authors: Ahmed
authors: Kazuhiko
authors: K
authors: Ishihara
authors: Ravin
authors: R
authors: Narain
mesh_terms: D002118:Calcium / Q000737:chemistry*; D066246:ErbB Receptors / Q000235:genetics*; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D008689:Methacrylates / Q000008:administration & dosage / Q000737:chemistry*; D010767:Phosphorylcholine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000737:chemistry*
chemical_list: D008689:Methacrylates; D034741:RNA, Small Interfering; D010767:Phosphorylcholine; C070638:2-methacryloyloxyethyl phosphorylcholine; D066246:ErbB Receptors; D002118:Calcium