title: Effects of waterborne cadmium on thyroid hormone levels and related gene expression in Chinese rare minnow larvae.
abstract: Cadmium is a heavy metal abundant in the environment that can induce endocrine disorder and toxicity in aquatic organisms at low levels. However, its effects on the thyroid system in fish are still unclear. In this study, the thyroid hormone (TH) levels and the expression profiles of genes related to hypothalamic- pituitary-thyroid (HPT) axis, including corticotropin-releasing hormone (crh), thyroid stimulating hormone beta (tshβ), solute carrier family 5 (sodium iodide symporter) member 5 (slc5a5), thyroglobulin (tg), thyroid hormone receptor alpha (trα) and thyroid hormone receptor beta (trβ), were determined in whole body of Chinese rare minnow (Gobiocypris rarus) larvae after exposure to different levels of Cd(2+) (0, 0.5 and 2.5mg/L) for 4days. And the 96-h lethal concentration of Cd(2+) on rare minnow larvae was determined as 2.59mg/L. The results showed that crh, slc5a5, tg and tshβ mRNA levels were significantly up-regulated in the larvae, but the gene expression of trα and trβ was down-regulated in a concentration-dependent manner. Besides, the THs levels decreased in the whole-body of fish, especially the thyroxine (T4) level. The above results indicated that Cd(2+) could alter gene expression in the HPT axis that might subsequently contribute to thyroid disruption.
authors: Key Laboratory of Freshwater Biodiversity Conservation (Ministry of Agriculture), Key Field Station for Fishery Resource and Environment in Upper-Middle Reaches of Yangtze River (Ministry of Agriculture), Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic. Electronic address: zhihuali06@yahoo.com.
authors: Zhi-Hua
authors: ZH
authors: Li
authors: Key Laboratory of Freshwater Biodiversity Conservation (Ministry of Agriculture), Key Field Station for Fishery Resource and Environment in Upper-Middle Reaches of Yangtze River (Ministry of Agriculture), Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
authors: Lu
authors: L
authors: Chen
authors: Key Laboratory of Freshwater Biodiversity Conservation (Ministry of Agriculture), Key Field Station for Fishery Resource and Environment in Upper-Middle Reaches of Yangtze River (Ministry of Agriculture), Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing 400715, China.
authors: Yan-Hua
authors: YH
authors: Wu
authors: Key Laboratory of Freshwater Biodiversity Conservation (Ministry of Agriculture), Key Field Station for Fishery Resource and Environment in Upper-Middle Reaches of Yangtze River (Ministry of Agriculture), Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.
authors: Ping
authors: P
authors: Li
authors: Key Laboratory of Freshwater Biodiversity Conservation (Ministry of Agriculture), Key Field Station for Fishery Resource and Environment in Upper-Middle Reaches of Yangtze River (Ministry of Agriculture), Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
authors: Yun-Feng
authors: YF
authors: Li
authors: Key Laboratory of Freshwater Biodiversity Conservation (Ministry of Agriculture), Key Field Station for Fishery Resource and Environment in Upper-Middle Reaches of Yangtze River (Ministry of Agriculture), Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China. Electronic address: nizhaoh@yfi.ac.cn.
authors: Zhao-Hui
authors: ZH
authors: Ni
mesh_terms: D000818:Animals; D002104:Cadmium / Q000009:adverse effects*; D002105:Cadmium Poisoning; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D004781:Environmental Exposure; D005399:Fishes / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects / Q000378:metabolism; D007814:Larva / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D013961:Thyroid Gland / Q000187:drug effects / Q000378:metabolism; D013963:Thyroid Hormones / Q000378:metabolism*; D013974:Thyroxine / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics; D014874:Water Pollutants, Chemical / Q000506:poisoning*
chemical_list: D012333:RNA, Messenger; D013963:Thyroid Hormones; D014874:Water Pollutants, Chemical; D002104:Cadmium; D013974:Thyroxine
keywords: Cadmium; Chinese rare minnow; Gene expression; Hypothalamic–pituitary–thyroid axis; Thyroid hormone