title: Magnetic ligation method for quantitative detection of microRNAs.
abstract: A magnetic ligation method is utilized for the detection of microRNAs among a complex biological background without polymerase chain reaction or nucleotide modification. The sandwich probes assay can be adapted to analyze a panel of microRNAs associated with cardiovascular diseases in heart tissue samples.
authors: Center for Systems Biology, Massachusetts General Hospital-Harvard Medical School, 185 Cambridge St., Boston, MA, 02114, USA.
authors: Monty
authors: M
authors: Liong
authors: Hyungsoon
authors: H
authors: Im
authors: Maulik D
authors: MD
authors: Majmudar
authors: Aaron D
authors: AD
authors: Aguirre
authors: Matthew
authors: M
authors: Sebas
authors: Hakho
authors: H
authors: Lee
authors: Ralph
authors: R
authors: Weissleder
mesh_terms: D011088:DNA Ligases; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D008863:Microspheres; D015336:Molecular Probe Techniques; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*
chemical_list: D058185:Magnetite Nanoparticles; D035683:MicroRNAs; D011088:DNA Ligases
keywords: diagnostics; enzymatic ligation; magnetic labeling; magnetic resonance; microRNA