title: Reprogramming to a pluripotent state modifies mesenchymal stem cell resistance to oxidative stress.
abstract: Properties of induced pluripotent stem cells (iPSC) have been extensively studied since their first derivation in 2006. However, the modification in reactive oxygen species (ROS) production and detoxification caused by reprogramming still needs to be further elucidated. The objective of this study was to compare the response of iPSC generated from menstrual blood-derived mesenchymal stem cells (mb-iPSC), embryonic stem cells (H9) and adult menstrual blood-derived mesenchymal stem cells (mbMSC) to ROS exposure and investigate the effects of reprogramming on cellular oxidative stress (OS). mbMSC were extremely resistant to ROS exposure, however, mb-iPSC were 10-fold less resistant to H(2)O(2), which was very similar to embryonic stem cell sensitivity. Extracellular production of ROS was also similar in mb-iPSC and H9 and almost threefold lower than in mbMSC. Furthermore, intracellular amounts of ROS were higher in mb-iPSC and H9 when compared with mbMSC. As the ability to metabolize ROS is related to antioxidant enzymes, we analysed enzyme activities in these cell types. Catalase and superoxide dismutase activities were reduced in mb-iPSC and H9 when compared with mbMSC. Finally, cell adhesion under OS conditions was impaired in mb-iPSC when compared with mbMSC, albeit similar to H9. Thus, reprogramming leads to profound modifications in extracellular ROS production accompanied by loss of the ability to handle OS.
authors: Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors: Karina D
authors: KD
authors: Asensi
authors: Rodrigo S
authors: RS
authors: Fortunato
authors: Danúbia S
authors: DS
authors: dos Santos
authors: Thaísa S
authors: TS
authors: Pacheco
authors: Danielle F
authors: DF
authors: de Rezende
authors: Deivid C
authors: DC
authors: Rodrigues
authors: Fernanda C P
authors: FC
authors: Mesquita
authors: Tais H
authors: TH
authors: Kasai-Brunswick
authors: Antonio C Campos
authors: AC
authors: de Carvalho
authors: Denise P
authors: DP
authors: Carvalho
authors: Adriana B
authors: AB
authors: Carvalho
authors: Regina C dos S
authors: RC
authors: Goldenberg
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D002448:Cell Adhesion; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D065150:Cellular Reprogramming; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007621:Karyotyping; D008598:Menstruation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008648:Mesoderm / Q000166:cytology; D018384:Oxidative Stress; D010641:Phenotype; D039904:Pluripotent Stem Cells / Q000166:cytology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013997:Time Factors
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species
keywords: induced pluripotent stem cells; menstrual blood; mesenchymal stem cells; oxidative stress; reactive oxygen species