title: [Studies on light-dependent nitrite reduction in Chlorella].
abstract: 1. The dependence of nitrite reduction activity in Chlorella fusca SHIHIRA et KRAUSS, as measured by uptake of nitrite, on the developmental stage, light intensity and CO2 concentration was investigated. 2. There is a quantitative relation between uptake of nitrite-nitrogen and increase of organic cell-nitrogen. 3. The kinetics of nitrite reduction in light are linear with time at high CO2 concentrations (3% in air) but biphasic at low CO2 concentrations (0 to 0.03%). In the steady state phase nitrite uptake is stimulated by an increase in the CO2 concentration from 0 to 0.03%. The initial phase and the steady state phase exhibit a different temperature dependence. 4. Nitrite uptake in the steady state phase is completely inhibited by iodoacetamide at concentrations which inhibit the photosynthetic CO2 reduction (5×10(-5) M). At lower iodoacetamide concentrations (1 to 2×10(-5) M) the linear time course of nitrite uptake at high CO2 concentrations is changed in the same manner as it is when the CO2 concentration is lowered. It is concluded that under special conditions (e.g. low CO2 concentration) the rate of steady state nitrite reduction in light is limited by the supply of carbon skeleton (i.e. N acceptors in the synthesis of organic nitrogen compounds) from the photosynthetic CO2 reduction cycle. 5. Pre-illumination in the presence of nitrite followed by a dark period depresses the initial phase of nitrite uptake in light at low CO2 concentrations. Pretreatment with nitrite in the dark lowers the nitrite uptake of the cells in the initial phase by exactly the same amount of nitrite which has been taken up during the pre-darkening period. It is concluded that there is a correlation between the initial phase of nitrite reduction in light and the respiration-linked nitrite reduction in the dark.
authors: Botanisches Institut der Universität Frankfurt/Main, Frankfurt/Main, Deutschland.
authors: H
authors: H
authors: Strotmann