title: Studying mechanosensitive ion channels with an automated patch clamp.
abstract: Patch clamp electrophysiology is the main technique to study mechanosensitive ion channels (MSCs), however, conventional patch clamping is laborious and success and output depends on the skills of the operator. Even though automated patch systems solve these problems for other ion channels, they could not be applied to MSCs. Here, we report on activation and single channel analysis of a bacterial mechanosensitive ion channel using an automated patch clamp system. With the automated system, we could patch not only giant unilamellar liposomes but also giant Escherichia coli (E. coli) spheroplasts. The tension sensitivity and channel kinetics data obtained in the automated system were in good agreement with that obtained from the conventional patch clamp. The findings will pave the way to high throughput fundamental and drug screening studies on mechanosensitive ion channels.
authors: Nanion Technologies GmbH, Gabrielenstr. 9, 80636, Munich, Germany.
authors: Maria
authors: M
authors: Barthmes
authors: Mac Donald F
authors: MD
authors: Jose
authors: Jan Peter
authors: JP
authors: Birkner
authors: Andrea
authors: A
authors: Brüggemann
authors: Christian
authors: C
authors: Wahl-Schott
authors: Armağan
authors: A
authors: Koçer
mesh_terms: D057205:Automation, Laboratory / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000378:metabolism*; D007473:Ion Channels / Q000378:metabolism*; D018408:Patch-Clamp Techniques / Q000379:methods*; D013104:Spheroplasts / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D007473:Ion Channels; C469542:MscS protein, E coli