title: Prevention of H2O2-induced oxidative stress in Chang liver cells by 4-hydroxybenzyl-chitooligomers.
abstract: In this study, a bioactive derivative of chitooligomers (1.0-3.0 kDa), 4-hydroxybenzyl-COS (HB-COS), was synthesized to enhance antioxidant activity. Hence, HB-COS was evaluated for its capabilities against H2O2-induced oxidative stress in human Chang liver cells. It was found that HB-COS possessed the free radical scavenging activity via decreasing the intracellular reactive oxygen species production. Furthermore, HB-COS significantly reduced the oxidation of DNA in a dose-dependent manner. Notably, HB-COS treatment upregulated the gene and protein expressions of antioxidative enzymes and thereby enhancing the intracellular antioxidant mechanisms. In addition, HB-COS treatment caused a remarkable blockade on degradation of inhibitory kappa B alpha (IκB-α) protein and translocation of nuclear factor kappa B (NF-κB). The current study demonstrated that HB-COS effectively attenuated hydrogen peroxide-induced oxidative stress in Chang liver cells by increasing levels of antioxidant enzymes and inhibiting reactive oxygen species generation, DNA oxidation and the NF-κB signaling pathway.
authors: Department of Biochemistry, Faculty of Biology, University of Science, VietNam National University Ho Chi Minh City (VNU-HCM), Ho Chi Minh City, Viet Nam.
authors: Mai Duy Luu
authors: MD
authors: Trinh
authors: Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Republic of Korea.
authors: Dai-Hung
authors: DH
authors: Ngo
authors: Department of Biochemistry, Faculty of Biology, University of Science, VietNam National University Ho Chi Minh City (VNU-HCM), Ho Chi Minh City, Viet Nam.
authors: Dang-Khoa
authors: DK
authors: Tran
authors: Department of Biochemistry, Faculty of Biology, University of Science, VietNam National University Ho Chi Minh City (VNU-HCM), Ho Chi Minh City, Viet Nam.
authors: Quoc-Tuan
authors: QT
authors: Tran
authors: Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Republic of Korea.
authors: Thanh-Sang
authors: TS
authors: Vo
authors: Department of Science and Technology, Ho Chi Minh City, Viet Nam.
authors: Minh-Hiep
authors: MH
authors: Dinh
authors: Department of Biochemistry, Faculty of Biology, University of Science, VietNam National University Ho Chi Minh City (VNU-HCM), Ho Chi Minh City, Viet Nam. Electronic address: nghiep75@yahoo.com.
authors: Dai-Nghiep
authors: DN
authors: Ngo
mesh_terms: D000975:Antioxidants / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D001559:Benzhydryl Compounds / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000494:pharmacology; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000975:Antioxidants; D001559:Benzhydryl Compounds; D016328:NF-kappa B; D017382:Reactive Oxygen Species; D004247:DNA; D048271:Chitosan; D006861:Hydrogen Peroxide
keywords: 4-Hydroxybenzyl-chitooligomers (HB-COS); Antioxidant enzymes; Chang liver cells; Free radical scavenging; Nuclear factor kappa B (NF-κB)