title: Phosphorylation of HPr by HPr kinase in Gluconobacter oxydans 621H.
abstract: DNA sequencing has revealed that Gluconobacter oxydans may contain an incomplete phosphoenolpyruvate: carbohydrate phosphotransferase system (PTS), but the function of individual members of the system remains unknown. Here we demonstrated that the predicted histidine protein HPr, an essential component of PTS, can be phosphorylated by a predicted HPr kinase in G. oxydans, where Ser54 in HPr is the site responsible for such a phosphorylation. The discovery implies that G. oxydans may regulate PTS activity in a way similar to that identified in some Gram-positive bacteria with low GC content.
authors: Ping
authors: P
authors: Zhang
authors: Yushu
authors: Y
authors: Ma
authors: Fengqing
authors: F
authors: Wang
authors: State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China. myushu@ecust.edu.cn.
authors: Dongzhi
authors: D
authors: Wei
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D020600:Gluconobacter oxydans / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005810:Multigene Family; D009154:Mutation; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D010770:Phosphotransferases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D010770:Phosphotransferases; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C030182:phosphocarrier protein HPr; C110847:HPr kinase; D017346:Protein Serine-Threonine Kinases