title: Human Sprouty1 suppresses growth, migration, and invasion in human breast cancer cells.
abstract: Breast cancer is the most common cancer and the leading cause of cancer death in women worldwide. Expression of human Sprouty1 (hSpry1) gene is downregulated in most breast cancer patients, implicating it as an important tumor suppressor gene. So, we hypothesized that overexpression of hSpry1 gene may suppress breast cancer cell growth, migration, and invasion. Here, we demonstrate that in breast cancer cell lines, MDA-MB-231 and T47D, transfection-induced overexpression of hSpry1 reduced cell population, proliferation, and colony formation in vitro without affecting cell apoptosis. Adhesion molecules act as both positive and negative modulators of cellular migration and invasion. Here, we found that overexpression of hSpry1 enhances the initial establishment events in breast cancer cell adhesion to type IV collagen and vitronectin. Moreover, the overexpression of hSpry1 in the highly invasive MDA-MB-231 breast cancer cells causes a significant reduction in cellular migration and invasion through Matrigel membranes. In addition, we showed that hSpry1 overexpression prevents VEGF secretion. VEGF is essential for primary tumor growth, migration, and invasion. Thus, our study provides a novel mechanism of tumor suppression activity of hSpry1.
authors: Cancer Research Laboratories, University of New South Wales Department of Surgery, Sydney, NSW 2217, Australia.
authors: Ahmed H
authors: AH
authors: Mekkawy
authors: Mohammad H
authors: MH
authors: Pourgholami
authors: David L
authors: DL
authors: Morris
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D024141:Collagen Type IV / Q000032:analysis; D005260:Female; D006801:Humans; D008565:Membrane Proteins / Q000502:physiology*; D009361:Neoplasm Invasiveness; D010750:Phosphoproteins / Q000502:physiology*; D042461:Vascular Endothelial Growth Factor A / Q000502:physiology
chemical_list: D024141:Collagen Type IV; D008565:Membrane Proteins; D010750:Phosphoproteins; C486055:SPRY1 protein, human; D042461:Vascular Endothelial Growth Factor A