title: Real-time visualization of 3-D dynamic microscopic objects using optical diffraction tomography.
abstract: 3-D refractive index (RI) distribution is an intrinsic bio-marker for the chemical and structural information about biological cells. Here we develop an optical diffraction tomography technique for the real-time reconstruction of 3-D RI distribution, employing sparse angle illumination and a graphic processing unit (GPU) implementation. The execution time for the tomographic reconstruction is 0.21 s for 96(3) voxels, which is 17 times faster than that of a conventional approach. We demonstrated the real-time visualization capability with imaging the dynamics of Brownian motion of an anisotropic colloidal dimer and the dynamic shape change in a red blood cell upon shear flow.
authors: Kyoohyun
authors: K
authors: Kim
authors: Kyung Sang
authors: KS
authors: Kim
authors: Hyunjoo
authors: H
authors: Park
authors: Jong Chul
authors: JC
authors: Ye
authors: Yongkeun
authors: Y
authors: Park
mesh_terms: D000465:Algorithms; D058948:Cell Tracking / Q000295:instrumentation*; D002478:Cells, Cultured; D003199:Computer Systems; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004912:Erythrocytes / Q000166:cytology*; D006801:Humans; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000295:instrumentation* / Q000379:methods; D012031:Refractometry / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D041622:Tomography, Optical / Q000295:instrumentation* / Q000379:methods