title: Effect of peripheral 5-HT on glucose and lipid metabolism in wether sheep.
abstract: In mice, peripheral 5-HT induces an increase in the plasma concentrations of glucose, insulin and bile acids, and a decrease in plasma triglyceride, NEFA and cholesterol concentrations. However, given the unique characteristics of the metabolism of ruminants relative to monogastric animals, the physiological role of peripheral 5-HT on glucose and lipid metabolism in sheep remains to be established. Therefore, in this study, we investigated the effect of 5-HT on the circulating concentrations of metabolites and insulin using five 5-HT receptor (5HTR) antagonists in sheep. After fasting for 24 h, sheep were intravenously injected with 5-HT, following which-, plasma glucose, insulin, triglyceride and NEFA concentrations were significantly elevated. In contrast, 5-HT did not affect the plasma cholesterol concentration, and it induced a decrease in bile acid concentrations. Increases in plasma glucose and insulin concentrations induced by 5-HT were attenuated by pre-treatment with Methysergide, a 5HTR 1, 2 and 7 antagonist. Additionally, decreased plasma bile acid concentrations induced by 5-HT were blocked by pre-treatment with Ketanserin, a 5HTR 2A antagonist. However, none of the 5HTR antagonists inhibited the increase in plasma triglyceride and NEFA levels induced by 5-HT. On the other hand, mRNA expressions of 5HTR1D and 1E were observed in the liver, pancreas and skeletal muscle. These results suggest that there are a number of differences in the physiological functions of peripheral 5-HT with respect to lipid metabolism between mice and sheep, though its effect on glucose metabolism appears to be similar between these species.
authors: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors: Hitoshi
authors: H
authors: Watanabe
authors: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors: Ryo
authors: R
authors: Saito
authors: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors: Tatsuya
authors: T
authors: Nakano
authors: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors: Hideyuki
authors: H
authors: Takahashi
authors: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors: Yu
authors: Y
authors: Takahashi
authors: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors: Keisuke
authors: K
authors: Sumiyoshi
authors: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors: Katsuyoshi
authors: K
authors: Sato
authors: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors: Xiangning
authors: X
authors: Chen
authors: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors: Natsumi
authors: N
authors: Okada
authors: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors: Shunsuke
authors: S
authors: Iwasaki
authors: Department of Animal Sciences, Faculty of Agriculture, Iwate University, Morioka, Japan.
authors: Dian W
authors: DW
authors: Harjanti
authors: Department of Animal Sciences, Faculty of Agriculture, Iwate University, Morioka, Japan.
authors: Natsumi
authors: N
authors: Sekiguchi
authors: Department of Animal Sciences, Faculty of Agriculture, Iwate University, Morioka, Japan.
authors: Hiroaki
authors: H
authors: Sano
authors: Laboratory of Food and Biomolecular Science, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors: Haruki
authors: H
authors: Kitazawa
authors: Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Cardiganshire, United Kingdom.
authors: Michael T
authors: MT
authors: Rose
authors: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors: Shyuichi
authors: S
authors: Ohwada
authors: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors: Kouichi
authors: K
authors: Watanabe
authors: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors: Hisashi
authors: H
authors: Aso
mesh_terms: D000818:Animals; D001647:Bile Acids and Salts / Q000097:blood; D001786:Blood Glucose / Q000187:drug effects*; D002784:Cholesterol / Q000097:blood; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000097:blood; D050356:Lipid Metabolism / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism; D011985:Receptors, Serotonin / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism* / Q000494:pharmacology*; D012702:Serotonin Antagonists / Q000494:pharmacology; D012756:Sheep / Q000097:blood / Q000378:metabolism*; D014280:Triglycerides / Q000097:blood
chemical_list: D001647:Bile Acids and Salts; D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D011985:Receptors, Serotonin; D012702:Serotonin Antagonists; D014280:Triglycerides; D012701:Serotonin; D002784:Cholesterol; D005947:Glucose