title: Method for the generation of antibodies specific for site and posttranslational modifications.
abstract: Protein phosphorylation plays critical roles in multiple aspects of cellular events. Site- and phosphorylation state-specific antibodies are indispensable to analyze spatially and temporally distribution of protein phosphorylation in cells. Such information provides some clues of its biological function. Here, we describe a strategy to design a phosphopeptide as an antigen for a site- and phosphorylation state-specific antibody. Importantly, this strategy is also applicable to the production of other types of antibodies, which specifically recognize the site-specific modification, such as acetylation, methylation, and proteolysis. This protocol also focuses on the screening for monoclonal version of a site- and phosphorylation state-specific antibody.
authors: Aichi Cancer Center Research Institute, Nagoya, Aichi, Japan.
authors: Hidemasa
authors: H
authors: Goto
authors: Masaki
authors: M
authors: Inagaki
mesh_terms: D000906:Antibodies / Q000276:immunology*; D010748:Phosphopeptides / Q000276:immunology; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational
chemical_list: D000906:Antibodies; D010748:Phosphopeptides