title: A study of the inhibition of catalase by dipotassium trioxohydroxytetrafluorotriborate K₂[B₃O₃F₄OH].
abstract: In the development of boronic acid-based enzyme inhibitors as potential pharmaceutical drugs, dipotassium trioxohydroxytetrafluorotriborate K2[B3O3F4OH] was listed as a promising new therapeutic for treatment of these diseases. The catalase-mediated conversion of hydrogen peroxide, in the presence and absence of K2[B3O3F4OH] was studied. The kinetics conformed to the Michaelis-Menten model. Lineweaver-Burk plots were linear and plotted the family of straight lines intersected on the abscissa indicating non-competitive inhibition of the catalase. It appears that in the absence of inhibitor, catalase operates the best at conditions around pH 7.1 and in the presence of K2[B3O3F4OH] the optimum is around pH 6.2. The uncatalyzed reaction of hydrogen peroxide decomposition generally has a value of activation energy of 75 kJ mole(-1), whereas catalase, in the absence of inhibitor, lowers the value to 11.2 kJ mole(-1), while in the presence 69 mmoles L(-1) of K2[B3O3F4OH] it was 37.8 kJ mole(-1).
authors: Department of Chemistry, Faculty of Science, University of Sarajevo , Sarajevo , Bosnia and Herzegovina and.
authors: Safija
authors: S
authors: Islamovic
authors: Borivoj
authors: B
authors: Galic
authors: Mladen
authors: M
authors: Milos
mesh_terms: D000818:Animals; D001896:Boron Compounds / Q000737:chemistry / Q000494:pharmacology*; D002374:Catalase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D015394:Molecular Structure; D013329:Structure-Activity Relationship
chemical_list: D001896:Boron Compounds; D004791:Enzyme Inhibitors; C561431:dipotassium trioxohydroxytetrafluorotriborate; D006861:Hydrogen Peroxide; D002374:Catalase
keywords: Boroxine; catalase; drug research; enzyme inhibition