title: The BAF60 subunit of the SWI/SNF chromatin-remodeling complex directly controls the formation of a gene loop at FLOWERING LOCUS C in Arabidopsis.
abstract: SWI/SNF complexes mediate ATP-dependent chromatin remodeling to regulate gene expression. Many components of these complexes are evolutionarily conserved, and several subunits of Arabidopsis thaliana SWI/SNF complexes are involved in the control of flowering, a process that depends on the floral repressor FLOWERING LOCUS C (FLC). BAF60 is a SWI/SNF subunit, and in this work, we show that BAF60, via a direct targeting of the floral repressor FLC, induces a change at the high-order chromatin level and represses the photoperiod flowering pathway in Arabidopsis. BAF60 accumulates in the nucleus and controls the formation of the FLC gene loop by modulation of histone density, composition, and posttranslational modification. Physiological analysis of BAF60 RNA interference mutant lines allowed us to propose that this chromatin-remodeling protein creates a repressive chromatin configuration at the FLC locus.
authors: Institut de Biologie des Plantes, Unité Mixte de Recherche 8618 Université Paris-Sud XI, 91405 Orsay, France.
authors: Teddy
authors: T
authors: Jégu
authors: David
authors: D
authors: Latrasse
authors: Marianne
authors: M
authors: Delarue
authors: Heribert
authors: H
authors: Hirt
authors: Séverine
authors: S
authors: Domenichini
authors: Federico
authors: F
authors: Ariel
authors: Martin
authors: M
authors: Crespi
authors: Catherine
authors: C
authors: Bergounioux
authors: Cécile
authors: C
authors: Raynaud
authors: Moussa
authors: M
authors: Benhamed
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly; D003080:Cold Temperature; D035264:Flowers / Q000235:genetics / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006657:Histones / Q000378:metabolism; D026161:MADS Domain Proteins / Q000235:genetics* / Q000378:metabolism; D008954:Models, Biological; D009690:Nucleic Acid Conformation; D017440:Photoperiod; D011499:Protein Processing, Post-Translational; D021122:Protein Subunits / Q000378:metabolism*; D034622:RNA Interference; D012319:RNA Polymerase II / Q000378:metabolism; D013997:Time Factors
chemical_list: D029681:Arabidopsis Proteins; D002843:Chromatin; C418902:At5g10140 protein, Arabidopsis; D006657:Histones; D026161:MADS Domain Proteins; D021122:Protein Subunits; D012319:RNA Polymerase II