title: Astaxanthin treatment confers protection against oxidative stress in U937 cells stimulated with lipopolysaccharide reducing O2- production.
abstract: Recently, astaxanthin (ASTA) studies have focused on several biological functions such as radical scavenging, singlet oxygen quenching, anti-carcinogenesis, anti-diabetic, anti-obesity, anti-inflammatory, anti-melanogenesis, and immune enhancement activities. In this study, we investigated the potential role protective of ASTA, an antioxidant marine carotenoid, in restoring physiological conditions in U937 cells stimulated with LPS (10 µg/ml). Our results show that pre-treatment with ASTA (10 µM) for 1 h attenuates the LPS-induced toxicity and ROS production. The beneficial effect of ASTA is associated with a reduction intracellular O2 (-) production by restoring the antioxidant network activity of superoxide dismutase (SOD) and catalase (CAT), which influence HO-1 expression and activity by inhibiting nuclear translocation of Nrf2. We accordingly hypothesize that ASTA has therapeutic properties protecting U937 cells from LPS-induced inflammatory and oxidative stress.
authors: Department of Medicine and Science of Aging, University G. D'Annunzio, Chieti, Italy.
authors: Sara
authors: S
authors: Franceschelli
authors: Department of Medicine and Science of Aging, University G. D'Annunzio, Chieti, Italy.
authors: Mirko
authors: M
authors: Pesce
authors: Department of Medicine and Science of Aging, University G. D'Annunzio, Chieti, Italy.
authors: Alessio
authors: A
authors: Ferrone
authors: Department of Medicine and Science of Aging, University G. D'Annunzio, Chieti, Italy.
authors: Maria Anna
authors: MA
authors: De Lutiis
authors: Department of Medicine and Science of Aging, University G. D'Annunzio, Chieti, Italy.
authors: Antonia
authors: A
authors: Patruno
authors: Department of Psychological, Humanistic and Territorial Sciences, University G. D'Annunzio, Chieti, Italy.
authors: Alfredo
authors: A
authors: Grilli
authors: Department of Medicine and Science of Aging, University G. D'Annunzio, Chieti, Italy.
authors: Mario
authors: M
authors: Felaco
authors: Department of Medicine and Science of Aging, University G. D'Annunzio, Chieti, Italy.
authors: Lorenza
authors: L
authors: Speranza
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D051547:Heme Oxygenase-1 / Q000378:metabolism; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D020011:Protective Agents / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*; D020298:U937 Cells; D024341:Xanthophylls / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D008070:Lipopolysaccharides; D051267:NF-E2-Related Factor 2; C495635:NFE2L2 protein, human; D020011:Protective Agents; D024341:Xanthophylls; D013481:Superoxides; D009580:Nitroblue Tetrazolium; C005948:astaxanthine; D002374:Catalase; D051547:Heme Oxygenase-1; D013482:Superoxide Dismutase