title: Effect of glucans from Caripia montagnei mushroom on TNBS-induced colitis.
abstract: In this study, we evaluated the effect of different doses of polysaccharides extracted from Caripia montagnei mushroom at different intervals of treatment on colonic injury in the model of colitis induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS). The FT-IR analysis and NMR showed that the polysaccharides from this species of mushroom are composed of α- and β-glucans. The colonic damage was evaluated by macroscopic, histological, biochemical and immunologic analyses. The results showed the reduction of colonic lesions in all groups treated with the glucans. Such glucans significantly reduced the levels of IL-6 (50 and 75 mg/kg, p < 0.05), a major inflammatory cytokine. Biochemical analyses showed that the glucans from C. montagnei acted on reducing levels of alkaline phosphatase (75 mg/kg, p < 0.01) and myeloperoxidase (p < 0.001), a result confirmed by the reduction of cellular infiltration observed microscopically. The increase of catalase activity possibly indicates a protective effect of these glucans on colonic tissue, confirming their anti-inflammatory potential.
authors: Laboratory of Glycobiology, Department of Biochemistry, Federal University of Rio Grande do Norte, Av. Salgado Filho, 3000, Bairro L. Nova, CEP 59078-970, Natal, RN, Brazil. mariliabio84@gmail.com.
authors: Marilia da S
authors: Mda S
authors: Nascimento Santos
authors: Laboratory of Glycobiology, Department of Biochemistry, Federal University of Rio Grande do Norte, Av. Salgado Filho, 3000, Bairro L. Nova, CEP 59078-970, Natal, RN, Brazil. joedyson_magalhaes@hotmail.com.
authors: Joedyson Emmanuel
authors: JE
authors: de M Magalhães
authors: Laboratory of Glycobiology, Department of Biochemistry, Federal University of Rio Grande do Norte, Av. Salgado Filho, 3000, Bairro L. Nova, CEP 59078-970, Natal, RN, Brazil. lsepwill_@gmail.com.
authors: Luiza Sheyla Evenni P Will
authors: LS
authors: Castro
authors: Laboratory of Glycobiology, Department of Biochemistry, Federal University of Rio Grande do Norte, Av. Salgado Filho, 3000, Bairro L. Nova, CEP 59078-970, Natal, RN, Brazil. tutu_sousa@hotmail.com.
authors: Thuane
authors: T
authors: de Sousa Pinheiro
authors: Department of Biochemistry, Federal University of Paraná, UFPR, Curitiba CEP 81531-980, PR, Brazil. popoh_diego@hotmail.com.
authors: Diego Araujo
authors: DA
authors: Sabry
authors: Laboratory of Glycobiology, Department of Biochemistry, Federal University of Rio Grande do Norte, Av. Salgado Filho, 3000, Bairro L. Nova, CEP 59078-970, Natal, RN, Brazil. leo.dnobre@gmail.com.
authors: Leonardo Thiago Duarte B
authors: LT
authors: Nobre
authors: Laboratory of Glycobiology, Department of Biochemistry, Federal University of Rio Grande do Norte, Av. Salgado Filho, 3000, Bairro L. Nova, CEP 59078-970, Natal, RN, Brazil. jplslima@gmail.com.
authors: João Paulo Matos Santos
authors: JP
authors: Lima
authors: Laboratory of Mycology, Department of Botany, Zoology and Ecology, Federal University of Rio Grande do Norte, Av. Salgado Filho, 3000, Bairro L. Nova 59078-970, Natal, RN, Brazil. baseia@cb.ufrn.br.
authors: Iuri Goulart
authors: IG
authors: Baseia
authors: Laboratory of Glycobiology, Department of Biochemistry, Federal University of Rio Grande do Norte, Av. Salgado Filho, 3000, Bairro L. Nova, CEP 59078-970, Natal, RN, Brazil. eddaleite@cb.ufrn.br.
authors: Edda Lisboa
authors: EL
authors: Leite
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002374:Catalase / Q000378:metabolism; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D016207:Cytokines / Q000096:biosynthesis; D004195:Disease Models, Animal; D004789:Enzyme Activation; D005936:Glucans / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D008297:Male; D009569:Nitric Oxide; D019906:Nuclear Magnetic Resonance, Biomolecular; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared; D014302:Trinitrobenzenesulfonic Acid / Q000009:adverse effects
chemical_list: D016207:Cytokines; D005936:Glucans; D009569:Nitric Oxide; D014302:Trinitrobenzenesulfonic Acid; D002374:Catalase; D009195:Peroxidase; D000469:Alkaline Phosphatase