title: Highly parallel transport recordings on a membrane-on-nanopore chip at single molecule resolution.
abstract: Membrane proteins are prime drug targets as they control the transit of information, ions, and solutes across membranes. Here, we present a membrane-on-nanopore platform to analyze nonelectrogenic channels and transporters that are typically not accessible by electrophysiological methods in a multiplexed manner. The silicon chip contains 250,000 femtoliter cavities, closed by a silicon dioxide top layer with defined nanopores. Lipid vesicles containing membrane proteins of interest are spread onto the nanopore-chip surface. Transport events of ligand-gated channels were recorded at single-molecule resolution by high-parallel fluorescence decoding.
authors: Institute of Biochemistry, Biocenter, Goethe-University , Frankfurt, Germany.
authors: Michael
authors: M
authors: Urban
authors: Alexander
authors: A
authors: Kleefen
authors: Nobina
authors: N
authors: Mukherjee
authors: Patrick
authors: P
authors: Seelheim
authors: Barbara
authors: B
authors: Windschiegl
authors: Marc
authors: M
authors: Vor der Brüggen
authors: Armagan
authors: A
authors: Koçer
authors: Robert
authors: R
authors: Tampé