title: Preparation and osteogenic differentiation of scaffold-free mouse adipose-derived stromal cell microtissue spheroids (ASC-MT).
abstract: In this unit, previously described methods are expanded upon, where procedures relating to the preparation, culturing, and osteogenic differentiation of scaffold-free mouse adipose-derived stromal cell microtissue spheroids (ASC-MT) are outlined. Not only is a detailed methodology of how to engineer such spheroids are presented, but a full account of how to induce and analyze osteogenesis in these ASC-MT constructs is given along with relevant figures to help better illustrate the methods described.
authors: Bone and Stem Cell Research Group, CABMM, University of Zurich, Zurich, Switzerland.
authors: Ali
authors: A
authors: Mirsaidi
authors: Bone and Stem Cell Research Group, CABMM, University of Zurich, Zurich, Switzerland.
authors: André N
authors: AN
authors: Tiaden
authors: Bone and Stem Cell Research Group, CABMM, University of Zurich, Zurich, Switzerland.
authors: Peter J
authors: PJ
authors: Richards
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation; D002478:Cells, Cultured; D008297:Male; D051379:Mice; D010012:Osteogenesis; D018874:Spheroids, Cellular / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology; D046509:Tissue Culture Techniques / Q000379:methods*; D054457:Tissue Scaffolds
keywords: 3D-culture; adipose-derived stromal cells; osteogenesis