title: Development of SPR biosensor for the detection of human hepatitis B virus using plasma-treated parylene-N film.
abstract: A plasma-treated parylene-N film was presented for the immobilization of proteins through physical adsorption. The changes in surface properties of the parylene-N film after plasma-treatment were analyzed using contact angle microscopy and AFM. To demonstrate the high protein-immobilization efficiency of the plasma-treated parylene-N film, the immobilization efficiencies of differently modified surfaces were compared using model proteins with different surface charges, such as streptavidin (pI=5, negatively charged at pH 7), horseradish peroxidase (pI=6.6, nearly neutral at pH 7), and avidin (pI=10, positively charged at pH 7). The application of the plasma-treated parylene-N film as an SPR biosensor was also tested by immobilizing model proteins. An SPR biosensor based on the plasma-treated parylene-N film was developed for the detection of the human hepatitis virus surface antigen (HBsAg), and the plasma-treated parylene-N film was estimated to improve the sensitivity of SPR biosensor as much as 1000-fold by enhancing immobilization of receptor antibodies.
authors: Department of Materials Science and Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-749, Republic of Korea.
authors: Yong-Hwan
authors: YH
authors: Choi
authors: Department of Materials Science and Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-749, Republic of Korea.
authors: Ga-Yeon
authors: GY
authors: Lee
authors: Department of Materials Science and Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-749, Republic of Korea.
authors: Hyuk
authors: H
authors: Ko
authors: Department of Materials Science and Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-749, Republic of Korea.
authors: Young Wook
authors: YW
authors: Chang
authors: Korea Institute of Science and Technology (KIST), Seoul, Republic of Korea.
authors: Min-Jung
authors: MJ
authors: Kang
authors: Department of Materials Science and Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-749, Republic of Korea. Electronic address: jcpyun@yonsei.ac.kr.
authors: Jae-Chul
authors: JC
authors: Pyun
mesh_terms: D000327:Adsorption; D006509:Hepatitis B / Q000821:virology; D006514:Hepatitis B Surface Antigens / Q000302:isolation & purification*; D006515:Hepatitis B virus / Q000302:isolation & purification*; D006801:Humans; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000302:isolation & purification; D020349:Surface Plasmon Resonance / Q000379:methods*; D013499:Surface Properties; D014992:Xylenes / Q000737:chemistry*
chemical_list: D006514:Hepatitis B Surface Antigens; D011108:Polymers; D011506:Proteins; D014992:Xylenes; C011055:parylene
keywords: Immobilization; Parylene-N; Plasma; SPR biosensor; Surface modification