title: Identification of Lactobacillus plantarum, Lactobacillus pentosus and Lactobacillus fermentum from honey stomach of honeybee.
abstract: This study aimed to isolate and identify Lactobacillus in the honey stomach of honeybee Apis dorsata. Samples of honeybee were collected from A. dorsata colonies in different bee trees and Lactobacillus bacteria isolated from honey stomachs. Ninety two isolates were Gram-stained and tested for catalase reaction. By using bacterial universal primers, the 16S rDNA gene from DNA of bacterial colonies amplified with polymerase chain reaction (PCR). Forty-nine bacterial 16S rDNA gene were sequenced and entrusted in GenBank. Phylogenetic analysis showed they were different phylotypes of Lactobacillus. Two of them were most closely relevant to the previously described species Lactobacillus plantarum. Other two phylotypes were identified to be closely related to Lactobacillus pentosus. However, only one phylotype was found to be distantly linked to the Lactobacillus fermentum. The outcomes of the present study indicated that L. plantarum, L. pentosus, and L. fermentum were the dominant lactobacilli in the honey stomach of honeybee A. dorsata collected during the dry season from Malaysia forest area - specifically "Melaleuca in Terengganu".
authors: Department of Food Sciences, Faculty of Food Sciences and Technology, University Putra Malaysia, Serdang, Selangor, Malaysia. ; Department of honey bee, Animal Science Research Institute of Iran, Karaj, Iran.
authors: Naser
authors: N
authors: Tajabadi
authors: Department of Agriculture Technology, Faculty of Agriculture, University Putra Malaysia, Serdang, Selangor, Malaysia.
authors: Makhdzir
authors: M
authors: Mardan
authors: Department of Food Sciences, Faculty of Food Sciences and Technology, University Putra Malaysia, Serdang, Selangor, Malaysia.
authors: Nazamid
authors: N
authors: Saari
authors: Departments of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, Serdang, Selangor, Malaysia.
authors: Shuhaimi
authors: S
authors: Mustafa
authors: Department of honey bee, Animal Science Research Institute of Iran, Karaj, Iran.
authors: Rasoul
authors: R
authors: Bahreini
authors: Department of Food Technology, Faculty of Food Sciences and Technology, University Putra Malaysia, Serdang, Selangor, Malaysia.
authors: Mohd Yazid Abdul
authors: MY
authors: Manap
mesh_terms: D000818:Animals; D001516:Bees / Q000382:microbiology*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D008296:Malaysia; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013270:Stomach / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: 16S rDNA gene sequences; Lactobacillus; honey bee; honey stomach; probiotic