title: Grape seed proanthocyanidin extract prevents DDP-induced testicular toxicity in rats.
abstract: Oxidative stress has been proven to be involved in cisplatin (DDP)-induced toxicity. The aim of the present study was to investigate a possible protective role of grape seed proanthocyanidin extract (GSPE) in DDP-induced spermiotoxicity. GSPE at 200 mg kg(-1) d(-1) and 400 mg kg(-1) d(-1) was orally administered for 15 consecutive days, starting 10 days before a single intraperitoneal dose of DDP (7 mg kg(-1)). Results revealed that testicular and epididymal weight, epididymal sperm count, motility and morphology, the activities of GSH-Px and SOD, and GSH levels were significantly decreased whereas the level of MDA was significantly increased in the DDP group rats. GSPE treatment significantly attenuated the harmful effects of DDP-induced lipid peroxidation, oxidative stress, loss of genital organ weight, as well as function of reproductive organs. These changes were restored to near normal levels by GSPE at 400 mg kg(-1) d(-1). In conclusion, GSPE has dose dependent protective effects against DDP-induced rat testicular toxicity.
authors: Beijing Key Laboratory of Bioactive Substances and Functional Foods, Beijing Union University, Beijing 100191, China. gaolip62@163.com.
authors: Yan-meng
authors: YM
authors: Zhao
authors: Li-ping
authors: LP
authors: Gao
authors: Hai-lian
authors: HL
authors: Zhang
authors: Jun-xia
authors: JX
authors: Guo
authors: Pei-pei
authors: PP
authors: Guo
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D002945:Cisplatin / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D056604:Grape Seed Extract / Q000008:administration & dosage*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D044945:Proanthocyanidins / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects; D013737:Testis / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D056604:Grape Seed Extract; C511402:Grape Seed Proanthocyanidins; D044945:Proanthocyanidins; D005978:Glutathione; D002945:Cisplatin