title: Actively targeted in vivo multiplex detection of intrinsic cancer biomarkers using biocompatible SERS nanotags.
abstract: Surface-enhanced Raman scattering (SERS) technique is becoming highly popular for multiplex biosensing due to the 'fingerprint' Raman spectra from every molecule. As a proof-of-concept, we demonstrated the actively targeted multiplex in vitro and in vivo detection of three intrinsic cancer biomarkers - EGFR, CD44 and TGFβRII in a breast cancer model using three multiplexing capable, biocompatible SERS nanoparticles/nanotags. Intra-tumorally injected antibody conjugated nanotags specifically targeting the three biomarkers exhibited maximum signal at 6 hours and no detectable signal at 72 hours. However, nanotags without antibodies showed no detectable signal after 6 hours. This difference could be due to the specific binding of the bioconjugated nanotags to the receptors on the cell surface. Thus, this study establishes SERS nanotags as an ultrasensitive nanoprobe for the multiplex detection of biomarkers and opens up its potential application in monitoring tumor progression and therapy and development into a theranostic probe.
authors: 1] Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), 11 Biopolis Way, Singapore 138667 [2].
authors: U S
authors: US
authors: Dinish
authors: 1] Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), 11 Biopolis Way, Singapore 138667 [2].
authors: Ghayathri
authors: G
authors: Balasundaram
authors: 1] Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), 11 Biopolis Way, Singapore 138667 [2] Department of Chemistry & MedChem Program of Life Sciences Institute, National University of Singapore, 117543 Singapore.
authors: Young-Tae
authors: YT
authors: Chang
authors: 1] Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), 11 Biopolis Way, Singapore 138667 [2] School of Physics, National University of Ireland Galway, Galway, Ireland.
authors: Malini
authors: M
authors: Olivo
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000276:immunology; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000276:immunology; D045744:Cell Line, Tumor; D066246:ErbB Receptors / Q000032:analysis / Q000276:immunology; D005260:Female; D006801:Humans; D018960:Hyaluronan Receptors / Q000032:analysis / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053758:Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000175:diagnosis; D017346:Protein Serine-Threonine Kinases / Q000032:analysis / Q000276:immunology; D000077294:Receptor, Transforming Growth Factor-beta Type II; D018125:Receptors, Transforming Growth Factor beta / Q000032:analysis / Q000276:immunology; D013059:Spectrum Analysis, Raman; D014183:Transplantation, Heterologous
chemical_list: D055910:Antibodies, Immobilized; D001672:Biocompatible Materials; D014408:Biomarkers, Tumor; D018960:Hyaluronan Receptors; D018125:Receptors, Transforming Growth Factor beta; D066246:ErbB Receptors; D017346:Protein Serine-Threonine Kinases; D000077294:Receptor, Transforming Growth Factor-beta Type II