title: The functional role of MnSOD as a biomarker of human diseases and therapeutic potential of a new isoform of a human recombinant MnSOD.
abstract: Reactive oxygen species (ROS) are generated as a consequence of metabolic reactions in the mitochondria of eukaryotic cells. This work describes the role of the manganese superoxide dismutase (MnSOD) as a biomarker of different human diseases and proposes a new therapeutic application for the prevention of cancer and its treatment. The paper also describes how a new form of human MnSOD was discovered, its initial application, and its clinical potentials. The MnSOD isolated from a human liposarcoma cell line (LSA) was able to kill cancer cells expressing estrogen receptors, but it did not have cytotoxic effects on normal cells. Together with its oncotoxic activity, the recombinant MnSOD (rMnSOD) exerts a radioprotective effect on normal cells irradiated with X-rays. The rMnSOD is characterized by the presence of a leader peptide, which allows the protein to enter cells: this unique property can be used in the radiodiagnosis of cancer or chemotherapy, conjugating radioactive substances or chemotherapic drugs to the leader peptide of the MnSOD. Compared to traditional chemotherapic agents, the drugs conjugated with the leader peptide of MnSOD can selectively reach and enter cancer cells, thus reducing the side effects of traditional treatments.
authors: Molecular Biology and Viral Oncology Unit, Department of Experimental Oncology, Istituto Nazionale per lo Studio e la Cura dei Tumori "Fondazione G. Pascale"-IRCCS, Naples, Italy.
authors: Antonella
authors: A
authors: Borrelli
authors: Molecular Biology and Viral Oncology Unit, Department of Experimental Oncology, Istituto Nazionale per lo Studio e la Cura dei Tumori "Fondazione G. Pascale"-IRCCS, Naples, Italy.
authors: Antonietta
authors: A
authors: Schiattarella
authors: Molecular Biology and Viral Oncology Unit, Department of Experimental Oncology, Istituto Nazionale per lo Studio e la Cura dei Tumori "Fondazione G. Pascale"-IRCCS, Naples, Italy.
authors: Patrizia
authors: P
authors: Bonelli
authors: Molecular Biology and Viral Oncology Unit, Department of Experimental Oncology, Istituto Nazionale per lo Studio e la Cura dei Tumori "Fondazione G. Pascale"-IRCCS, Naples, Italy.
authors: Franca Maria
authors: FM
authors: Tuccillo
authors: Molecular Biology and Viral Oncology Unit, Department of Experimental Oncology, Istituto Nazionale per lo Studio e la Cura dei Tumori "Fondazione G. Pascale"-IRCCS, Naples, Italy.
authors: Franco Maria
authors: FM
authors: Buonaguro
authors: Molecular Biology and Viral Oncology Unit, Department of Experimental Oncology, Istituto Nazionale per lo Studio e la Cura dei Tumori "Fondazione G. Pascale"-IRCCS, Naples, Italy.
authors: Aldo
authors: A
authors: Mancini
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers; D045744:Cell Line, Tumor; D006801:Humans; D008080:Liposarcoma / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D008928:Mitochondria / Q000378:metabolism* / Q000473:pathology; D058990:Molecular Targeted Therapy; D020033:Protein Isoforms / Q000235:genetics / Q000627:therapeutic use; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism / Q000627:therapeutic use
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D020033:Protein Isoforms; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase