title: Structural and genetic studies of the O-antigen of Enterobacter cloacae G2277.
abstract: The O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Enterobacter cloacae G2277 and studied by sugar analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy. The following structure of the linear pentasaccharide repeating unit was established, where a galacturonic acid (GalA) residue is mono-O-acetylated at position either 2 or 3: The O-antigen gene cluster of E. cloacae G2277 was sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in agreement with the O-polysaccharide structure.
authors: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. Electronic address: perepel@ioc.ac.ru.
authors: Andrei V
authors: AV
authors: Perepelov
authors: TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, PR China; Tianjin Key Laboratory for Microbial Functional Genomics, TEDA College, Nankai University, TEDA, Tianjin 300457, PR China.
authors: Min
authors: M
authors: Wang
authors: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors: Andrei V
authors: AV
authors: Filatov
authors: Tianjin Key Laboratory for Microbial Functional Genomics, TEDA College, Nankai University, TEDA, Tianjin 300457, PR China.
authors: Xi
authors: X
authors: Guo
authors: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors: Alexander S
authors: AS
authors: Shashkov
authors: TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, PR China; Tianjin Key Laboratory for Microbial Functional Genomics, TEDA College, Nankai University, TEDA, Tianjin 300457, PR China.
authors: Lei
authors: L
authors: Wang
authors: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors: Yuriy A
authors: YA
authors: Knirel
mesh_terms: D000107:Acetylation; D002240:Carbohydrate Sequence; D016972:Enterobacter cloacae / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D005810:Multigene Family; D019081:O Antigens / Q000737:chemistry* / Q000235:genetics*
chemical_list: D008070:Lipopolysaccharides; D019081:O Antigens
keywords: Bacterial polysaccharide structure; Enterobacter cloacae; Lipopolysaccharide; O-Antigen gene cluster; O-Polysaccharide