title: Visualizing genomes with Oligopaint FISH probes.
abstract: Oligopaint probes are fluorescently labeled, single-stranded DNA oligonucleotides that can be used to visualize genomic regions ranging in size from tens of kilobases to many megabases. This unit details how Oligopaint probes can be synthesized using basic molecular biological techniques, and provides protocols for FISH, 3D-FISH, and sample preparation.
authors: Department of Genetics, Harvard Medical School, Boston, Massachusetts.
authors: Brian J
authors: BJ
authors: Beliveau
authors: Nicholas
authors: N
authors: Apostolopoulos
authors: Chao-ting
authors: CT
authors: Wu
mesh_terms: D004277:DNA, Single-Stranded / Q000235:genetics*; D016678:Genome / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D015345:Oligonucleotide Probes / Q000235:genetics*; D009841:Oligonucleotides / Q000235:genetics*
chemical_list: D004277:DNA, Single-Stranded; D015345:Oligonucleotide Probes; D009841:Oligonucleotides
keywords: FISH; Oligopaint; complex DNA library; oligonucleotide