title: Microvasculature of carotid atheromatous plaques: hemorrhagic plaques have dense microvessels with fenestrations to the arterial lumen.
abstract: BACKGROUND
  Microvessels in atheromatous plaques are well known to play a role in plaque vulnerability associated with intraplaque hemorrhage, but their architecture remains unclear. The morphometry of the microvasculature and hemorrhage of human carotid atheromatous plaques (CAPs) were evaluated, and 3-dimensional (3D) reconstruction of the microvessels was performed.
  
  
  METHODS
  CAPs were obtained by endarterectomy in 42 patients. The specimens were analyzed using light microscopy. Plaque hemorrhage was defined as an area-containing red blood cells (>1 mm2). To determine the histopathologic features of plaque hemorrhage, the plaque area was divided into 4 regions: cap, shoulder, lipid/necrotic core, and media. Then, the density of microvessels and macrophages in each region was quantified. Two representative lesions with either hemorrhagic or nonhemorrhagic plaque were cut into 90 serial sections. The sections were double stained with anti-CD34 and anti-α smooth muscle actin antibodies, scanned using a digital microscope, and reconstructed using TRI-SRF2 software.
  
  
  RESULTS
  The hemorrhagic plaques showed a higher density of microvessels than nonhemorrhagic plaques in the shoulder, cap, and lipid/necrotic core (P=.03, .009, and .001, respectively), and there was positive correlations between its density and macrophages in each regions (P<.001, .001, and .019, respectively). 3D imaging also revealed dense microvessels with a network structure in the cap and shoulder regions of hemorrhagic plaques, and some of the vessels were fenestrated to the arterial lumen.
  
  
  CONCLUSIONS
  The microvasculature of plaques with intraplaque hemorrhage was dense, some of which fenestrated to the arterial lumen. The pathologic 3D imaging revealed precise architecture of microvasculature of plaques.
authors: Department of Pathology, Ehime University Graduate School of Medicine and Proteo-Science Center, Toon, Ehime, Japan; Department of Cardiology, Erasmus Medical Center Rotterdam, the Netherlands. Electronic address: miekrt@gmail.com.
authors: Mie
authors: M
authors: Kurata
authors: Department of Pathology, Division of Pathogenomics, Ehime University, Graduate School of Medicine, Ehime, Japan.
authors: Masato
authors: M
authors: Nose
authors: Department of Pathology, The Nippon Dental University, Tokyo, Japan.
authors: Yoshihito
authors: Y
authors: Shimazu
authors: Department of Pathology, The Nippon Dental University, Tokyo, Japan.
authors: Takaaki
authors: T
authors: Aoba
authors: Department of Pathology, Ehime University Graduate School of Medicine and Proteo-Science Center, Toon, Ehime, Japan.
authors: Yuki
authors: Y
authors: Kohada
authors: Department of Pathology, Ehime University Graduate School of Medicine and Proteo-Science Center, Toon, Ehime, Japan.
authors: Soichiro
authors: S
authors: Yorioka
authors: Department of Pathology, Ehime University Graduate School of Medicine and Proteo-Science Center, Toon, Ehime, Japan.
authors: Satomi
authors: S
authors: Suehiro
authors: Department of Pathology, Ehime University Graduate School of Medicine and Proteo-Science Center, Toon, Ehime, Japan.
authors: Erina
authors: E
authors: Fukuoka
authors: Department of Neurosurgery, Ehime University Graduate School of Medicine, Ehime, Japan.
authors: Shirabe
authors: S
authors: Matsumoto
authors: Department of Neurosurgery, Ehime University Graduate School of Medicine, Ehime, Japan.
authors: Hideaki
authors: H
authors: Watanabe
authors: Department of Neurosurgery, Ehime University Graduate School of Medicine, Ehime, Japan.
authors: Yoshiaki
authors: Y
authors: Kumon
authors: Department of Integrated Medicine and Informatics, Ehime University Graduate School of Medicine, Ehime, Japan.
authors: Takafumi
authors: T
authors: Okura
authors: Department of Integrated Medicine and Informatics, Ehime University Graduate School of Medicine, Ehime, Japan.
authors: Jitsuo
authors: J
authors: Higaki
authors: Department of Pathology, Ehime University Graduate School of Medicine and Proteo-Science Center, Toon, Ehime, Japan.
authors: Junya
authors: J
authors: Masumoto
mesh_terms: D002339:Carotid Arteries / Q000473:pathology* / Q000601:surgery; D016893:Carotid Stenosis / Q000473:pathology* / Q000601:surgery; D016894:Endarterectomy, Carotid; D006801:Humans; D055806:Microvessels; D058226:Plaque, Atherosclerotic / Q000473:pathology* / Q000601:surgery; D017540:Tunica Media / Q000473:pathology* / Q000601:surgery
keywords: Atherosclerosis; intraplaque hemorrhage; microvasculature of plaques; three-dimensional imaging