title: Anti-scorpion venom activity of Andrographis paniculata: A combined and comparative study with anti-scorpion serum in mice.
abstract: OBJECTIVE
  The objective of this study is to evaluate the anti-scorpion venom (ASV) property of Andrographis paniculata in comparison with anti-redscorpion venom serum and this study aimed to determine its combined effect with anti-redscorpion venom serum.
  
  
  METHODS
  Ethanolic extract of the plant AP was obtained using soxhlet apparatus. Swiss albino mice weighing 20-30g were used. Lyophilized venom sample of Mesobuthus tamulus and Lyophilized monovalent enzyme refined immunoglobulin anti-scorpion venom serum (ASV) was used. Using lethal dose of scorpion venom (25.12μg/g), the venom neutralizing ability of plant extract (1 g/kg) and ASV individually as well as in combination was studied using in vivo and in vitro methods. Mean survival time, protection fold and percentage survival of animals over the period of 24 h were the parameters used.
  
  
  METHODS
  Results were analyzed using Student's t-test.
  
  
  RESULTS
  Ethanolic extract of AP (1 g/kg) showed some protective effect against scorpion venom. ASV was found more effective than plant extract. But, when plant extract and ASV were used in combination, potency of ASV was found to be increased both in vivo and in vitro.
  
  
  CONCLUSIONS
  Present study demonstrates that, both plant extract and ASV have their own scorpion venom neutralising ability in vivo and in vitro, but their combination is most effective in venom neutralizing ability.
authors: Department of Pharmacology, Mahatma Gandhi Institute of Medical Sciences, Sewagram, Wardha, Maharshtra, India.
authors: Ranjana S
authors: RS
authors: Kale
authors: Department of Pharmacology, Mahatma Gandhi Institute of Medical Sciences, Sewagram, Wardha, Maharshtra, India.
authors: Satish E
authors: SE
authors: Bahekar
authors: Department of Pharmacology, Mahatma Gandhi Institute of Medical Sciences, Sewagram, Wardha, Maharshtra, India.
authors: Shailesh R
authors: SR
authors: Nagpure
authors: Department of Pharmacology, Mahatma Gandhi Institute of Medical Sciences, Sewagram, Wardha, Maharshtra, India.
authors: Kartik J
authors: KJ
authors: Salwe
keywords: Andrographis paniculata; Mesobuthus tamulus; anti-scorpion venom; lethal dose