title: Structural characteristics of water-soluble polysaccharides from Heracleum sosnowskyi Manden.
abstract: Fractions containing arabinogalactan proteins (AGPs) and pectic polysaccharides were isolated from above-ground parts of Heracleum sosnowskyi. Major units of their structure were elucidated using ion-exchange chromatography, gel chromatography, and NMR spectroscopy. The carbohydrate backbone of the polysaccharides consisted of 1,3-β-D-galactopyranosyl residues, whereas side chains of the branched region consisted of the residues of 1,6-β-galactopyranose, 1,5-α-L-arabinofuranose, 1,4-β-D-glucuronic acid, and 1,6-β-D-glucopyranose. The branching points were identified as 1,3,6-β-D-galactopyranose residues. Side chains were terminated with β-D-galactopyranose, α-L-arabinofuranose and α-L-rhamnopyranose. A significant part of the side-chain β-1,6-galactan was substituted at C6 by 4-OMe-β-D-glucuronic acid. A minor part of glucuronic acid was included in the α-Rhap-(1 → 4)-β-GlcA-(→ fragment. All the studied fractions contained 1,4-β-D-galacturonic acid as well.
authors: Institute of Chemistry, Komi Science Centre, The Urals Branch of the Russian Academy of Sciences, Pervomaiskaya Street 49, Syktyvkar 167982, Russia.
authors: Evgeny G
authors: EG
authors: Shakhmatov
authors: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, Moscow 119991, Russia.
authors: Philip V
authors: PV
authors: Toukach
authors: Institute of Chemistry, Komi Science Centre, The Urals Branch of the Russian Academy of Sciences, Pervomaiskaya Street 49, Syktyvkar 167982, Russia.
authors: Sergey P
authors: SP
authors: Kuznetsov
authors: Institute of Chemistry, Komi Science Centre, The Urals Branch of the Russian Academy of Sciences, Pervomaiskaya Street 49, Syktyvkar 167982, Russia. Electronic address: makarowa.elena-ma@yandex.ru.
authors: Elena N
authors: EN
authors: Makarova
mesh_terms: D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D030002:Heracleum / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D011134:Polysaccharides / Q000737:chemistry*; D012995:Solubility; D014867:Water / Q000737:chemistry
chemical_list: D011134:Polysaccharides; D014867:Water
keywords: Arabinogalactan protein; Glucomannan; Heracleum sosnowskyi; NMR; Pectin; Structure