title: Complete genome sequence of Dehalobacter restrictus PER-K23(T.).
abstract: Dehalobacter restrictus strain PER-K23 (DSM 9455) is the type strain of the species Dehalobacter restrictus. D. restrictus strain PER-K23 grows by organohalide respiration, coupling the oxidation of H2 to the reductive dechlorination of tetra- or trichloroethene. Growth has not been observed with any other electron donor or acceptor, nor has fermentative growth been shown. Here we introduce the first full genome of a pure culture within the genus Dehalobacter. The 2,943,336 bp long genome contains 2,826 protein coding and 82 RNA genes, including 5 16S rRNA genes. Interestingly, the genome contains 25 predicted reductive dehalogenase genes, the majority of which appear to be full length. The reductive dehalogenase genes are mainly located in two clusters, suggesting a much larger potential for organohalide respiration than previously anticipated.
authors: Wageningen University, Agrotechnology and Food Sciences, Laboratory of Microbiology, Dreijenplein 10, NL-6703 HB Wageningen, The Netherlands.
authors: Thomas
authors: T
authors: Kruse
authors: Ecole Polytechnique Fédérale de Lausanne (EPFL), School of Architecture, Civil and Environmental Engineering, Laboratory for Environmental Biotechnology, Station 6, CH-1015 Lausanne, Switzerland.
authors: Julien
authors: J
authors: Maillard
authors: DOE Joint Genome Institute, Walnut Creek, California, USA ; Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
authors: Lynne
authors: L
authors: Goodwin
authors: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors: Tanja
authors: T
authors: Woyke
authors: DOE Joint Genome Institute, Walnut Creek, California, USA ; Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
authors: Hazuki
authors: H
authors: Teshima
authors: DOE Joint Genome Institute, Walnut Creek, California, USA ; Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
authors: David
authors: D
authors: Bruce
authors: DOE Joint Genome Institute, Walnut Creek, California, USA ; Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
authors: Chris
authors: C
authors: Detter
authors: DOE Joint Genome Institute, Walnut Creek, California, USA ; Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
authors: Roxanne
authors: R
authors: Tapia
authors: DOE Joint Genome Institute, Walnut Creek, California, USA ; Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
authors: Cliff
authors: C
authors: Han
authors: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors: Marcel
authors: M
authors: Huntemann
authors: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors: Chia-Lin
authors: CL
authors: Wei
authors: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors: James
authors: J
authors: Han
authors: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors: Amy
authors: A
authors: Chen
authors: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors: Nikos
authors: N
authors: Kyrpides
authors: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors: Ernest
authors: E
authors: Szeto
authors: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors: Victor
authors: V
authors: Markowitz
authors: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors: Natalia
authors: N
authors: Ivanova
authors: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors: Ioanna
authors: I
authors: Pagani
authors: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors: Amrita
authors: A
authors: Pati
authors: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors: Sam
authors: S
authors: Pitluck
authors: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors: Matt
authors: M
authors: Nolan
authors: Ecole Polytechnique Fédérale de Lausanne (EPFL), School of Architecture, Civil and Environmental Engineering, Laboratory for Environmental Biotechnology, Station 6, CH-1015 Lausanne, Switzerland.
authors: Christof
authors: C
authors: Holliger
authors: Wageningen University, Agrotechnology and Food Sciences, Laboratory of Microbiology, Dreijenplein 10, NL-6703 HB Wageningen, The Netherlands.
authors: Hauke
authors: H
authors: Smidt
keywords: Dehalobacter restrictus type strain; PCE; TCE; anaerobe; organohalide respiration; reductive dehalogenases