title: Micromirror structured illumination microscope for high-speed in vivo drosophila brain imaging.
abstract: Genetic tools and especially genetically encoded fluorescent reporters have given a special place to optical microscopy in drosophila neurobiology research. In order to monitor neural networks activity, high speed and sensitive techniques, with high spatial resolution are required. Structured illumination microscopies are wide-field approaches with optical sectioning ability. Despite the large progress made with the introduction of the HiLo principle, they did not meet the criteria of speed and/or spatial resolution for drosophila brain imaging. We report on a new implementation that took advantage of micromirror matrix technology to structure the illumination. Thus, we showed that the developed instrument exhibits a spatial resolution close to that of confocal microscopy but it can record physiological responses with a speed improved by more than an order a magnitude.
authors: A
authors: A
authors: Masson
authors: M
authors: M
authors: Pedrazzani
authors: S
authors: S
authors: Benrezzak
authors: P
authors: P
authors: Tchenio
authors: T
authors: T
authors: Preat
authors: D
authors: D
authors: Nutarelli
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology*; D004330:Drosophila / Q000033:anatomy & histology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D007909:Lenses; D008029:Lighting / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D008904:Miniaturization