title: [Effect of DNA methyltransferase 1 and methyl-CpG-binding protein 2 abnormal expression on cervical lesions and related interaction].
abstract: OBJECTIVE
  To explore the effect of DNA methyltransferase 1 (DNMT1) and methyl-CpG-binding protein 2 (MeCP2) on cervical cancer and cervix precancerous lesion.
  
  
  METHODS
  74 patients with cervix squamous cell carcinoma(SCC), 52 patients with cervical intraepithelial neoplasm I (CIN I), 60 patients with cervical intraepithelial neoplasm II - II (CIN II-III)and 58 patients with histologically diagnosed cervix inflammation(CI), were included in this study. Information as demography, reproductive history, life style, HPV infection were collected. Western Blot were used to detect the expression of DNMT1 protein and MeCP2 protein. Real-time PCR was used to detect the expression of DNMT1 and MeCP2 mRNA.
  
  
  RESULTS
  Levels of DNMT1 and MeCP2 protein expression increased gradually with the deterioration of cervical lesion (H = 94.33, P < 0.001;F = 21.580, P < 0.001). Along with the deterioration of cervical lesion, levels of DNMT1 and MeCP2 mRNA expression were gradually increasing( F = 4.758, P = 0.003; F = 7.804, P < 0.001). Data from Correlation analysis showed that both protein (r = 0.287, P < 0.001) and mRNA(r = 0.179, P = 0.005)were positive correlated with DNMT1 and MeCP2.
  
  
  RESULTS
  of our study indicated that there was an additive interaction between high-expression of DNMT1 protein and high-expression of MeCP2 protein in SCC or CIN II-III. However, there was an additive interaction between high-expression of DNMT1 mRNA and high-expression of MeCP2 mRNA in SCC or CIN II-III.
  
  
  CONCLUSIONS
  Results from our study revealed the fact that both high expression of DNMT1 protein and high expression of MeCP2 protein could increase the risk of cervix cancerization. According to our findings, there might be a synergistic action existed between DNMT1 and MeCP2 during the progression of cervix cancelation.
authors: Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan 030001, China.
authors: Hui-jie
authors: HJ
authors: Kang
authors: Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan 030001, China.
authors: Jin-tao
authors: JT
authors: Wang
authors: Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan 030001, China.
authors: Chen-fei
authors: CF
authors: Gao
authors: Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan 030001, China.
authors: Li-xia
authors: LX
authors: Bai
authors: Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan 030001, China.
authors: Ling
authors: L
authors: Ding
authors: Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan 030001, China.
authors: Juan
authors: J
authors: Xu
authors: Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan 030001, China. Email: wangjt59@163.com.
authors: Ting-ting
authors: TT
authors: Wu
authors: Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan 030001, China.
authors: Lan
authors: L
authors: Bai
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D051783:Methyl-CpG-Binding Protein 2 / Q000378:metabolism*; D008875:Middle Aged; D019853:O(6)-Methylguanine-DNA Methyltransferase / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology; D002578:Uterine Cervical Dysplasia / Q000378:metabolism* / Q000473:pathology
chemical_list: D051783:Methyl-CpG-Binding Protein 2; D012333:RNA, Messenger; D019853:O(6)-Methylguanine-DNA Methyltransferase