title: Specialization of oleosins in oil body dynamics during seed development in Arabidopsis seeds.
abstract: Oil bodies (OBs) are seed-specific lipid storage organelles that allow the accumulation of neutral lipids that sustain plantlet development after the onset of germination. OBs are covered with specific proteins embedded in a single layer of phospholipids. Using fluorescent dyes and confocal microscopy, we monitored the dynamics of OBs in living Arabidopsis (Arabidopsis thaliana) embryos at different stages of development. Analyses were carried out with different genotypes: the wild type and three mutants affected in the accumulation of various oleosins (OLE1, OLE2, and OLE4), three major OB proteins. Image acquisition was followed by a detailed statistical analysis of OB size and distribution during seed development in the four dimensions (x, y, z, and t). Our results indicate that OB size increases sharply during seed maturation, in part by OB fusion, and then decreases until the end of the maturation process. In single, double, and triple mutant backgrounds, the size and spatial distribution of OBs are modified, affecting in turn the total lipid content, which suggests that the oleosins studied have specific functions in the dynamics of lipid accumulation.
authors: Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1318, AgroParisTech, and Equipe de Recherche Labellisée 3559 Centre National de la Recherche Scientifique, Institut Jean-Pierre Bourgin, F-78026 Versailles cedex, France.
authors: Martine
authors: M
authors: Miquel
authors: Ghassen
authors: G
authors: Trigui
authors: Sabine
authors: S
authors: d'Andréa
authors: Zsolt
authors: Z
authors: Kelemen
authors: Sébastien
authors: S
authors: Baud
authors: Adeline
authors: A
authors: Berger
authors: Carine
authors: C
authors: Deruyffelaere
authors: Alain
authors: A
authors: Trubuil
authors: Loïc
authors: L
authors: Lepiniec
authors: Bertrand
authors: B
authors: Dubreucq
mesh_terms: D017360:Arabidopsis / Q000196:embryology* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D018525:Germination; D021621:Imaging, Three-Dimensional; D002479:Inclusion Bodies / Q000378:metabolism*; D010641:Phenotype; D010938:Plant Oils / Q000378:metabolism*; D012044:Regression Analysis; D012639:Seeds / Q000254:growth & development* / Q000378:metabolism*; D013194:Staining and Labeling
chemical_list: D029681:Arabidopsis Proteins; D010938:Plant Oils