title: Effect of anesthesia on cognitive status and MMP-2 expression in rats.
abstract: OBJECTIVE
  To investigate the effect of anesthesia on the cognitive status damage and MMP-2 expression in rats.
  
  
  METHODS
  A total of 120 healthy rats were selected and randomly divided into the control group, CF3-CH(OCH2F)-CF3 (Sevoflurane) group and CF3-CH2-O-CHF-CF3 group (Sevoflurane) (n=40). After training for 3 d by the Morris water maze, the control group were injected with fentanyl for analgesia, the CF3-CH(OCH2F)-CF3 group and the CF3-CH2-O-CHF-CF3 group were anesthesia with CF3-CH (OCH2F)-CF3 and CF3-CH2-O-CHF-CF3 on the basis of fentanyl, then rats in three groups underwent open surgery and suture conventional incision. Morris water maze was used to measure the rats' cognitive ability in three groups on the 1st d, 3rd d, 5th d and 7th d, and the brain tissue MMP-2 expression was detected.
  
  
  RESULTS
  After 1 d/7 d of the surgery, Morris water maze performance and MMP-2 expression were not significantly different among three groups (P>0.05); After 3 d/5 d of the surgery, compared with the control group, the Morris water maze test result was significantly worsened, MMP-2 expression levels were significantly increased (P<0.05); After 3 d/5 d of the surgery, compared with the CF3-CH2-O-CHF-CF3 group, Morris water maze test result of CF3-CH(OCH2F)-CF3 group was significantly worsened, MMP-2 expression levels were significantly increased (P<0.05).
  
  
  CONCLUSIONS
  Anesthesia can cause some injury on cognitive status, different anesthetic drugs may cause different injury, and the cognitive status injury is related to the MMP-2 expression.
authors: Department of Anesthesiology, People's Hospital of Linzi District, Zibo 255400, Shandong Province, China.
authors: Hong-Tu
authors: HT
authors: Li
authors: Department of Anesthesiology, People's Hospital of Linzi District, Zibo 255400, Shandong Province, China.
authors: Quan-Jun
authors: QJ
authors: Cao
authors: Department of Urology, People's Hospital of Linzi District, Zibo 255400, Shandong Province, China.
authors: Xiang-Jie
authors: XJ
authors: Qi
authors: Department of Anesthesiology, People's Hospital of Linzi District, Zibo 255400, Shandong Province, China; Operating Room, People's Hospital of Linzi District, Zibo 255400, Shandong Province, China. Electronic address: Lht030506@sina.com.
authors: Wei-Ling
authors: WL
authors: Lu
mesh_terms: D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D001923:Brain Chemistry / Q000187:drug effects*; D003071:Cognition / Q000187:drug effects*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D018782:Maze Learning; D008738:Methyl Ethers / Q000494:pharmacology*; D011183:Postoperative Complications; D011897:Random Allocation; D051381:Rats; D000077149:Sevoflurane
chemical_list: D018685:Anesthetics, Inhalation; D008738:Methyl Ethers; D000077149:Sevoflurane; D020778:Matrix Metalloproteinase 2
keywords: Anesthesia; Cognition; Influence study; Matrix metalloproteinase-2