title: In vitro assay for the toxicity of silver nanoparticles using heart and gill cell lines of Catla catla and gill cell line of Labeo rohita.
abstract: Silver nanoparticles (Ag-NPs) are used in commercial products for their antimicrobial properties. The Ag-NPs in some of these products are likely to reach the aquatic environment, thereby posing a health concern for humans and aquatic species. The silver nanoparticles were synthesized and characterized using, UV-vis spectra, Dynamic light scattering (DLS) and Transmission electron microscopy (TEM) analysis. Acute toxicity tests on fish were conducted by exposing Catla catla and Labeo rohita for 96h to AgNO3 and Ag-NPs under static conditions. The cytotoxic effect of AgNO3 and Ag-NPs in Sahul India C. catla heart cell line (SICH), Indian C. catla gill cell line (ICG) and L. rohita gill cell line (LRG) was assessed using MTT and neutral red (NR) assay. Linear correlations between each in vitro EC50 and the in vivo LC50 data were highly significant. DNA damage and nuclear fragmentation (condensation) were assessed by comet assay and Hoechst staining, respectively in SICH, ICG and LRG cells exposed to Ag-NPs. The results of antioxidant parameter obtained show significantly increased lipid peroxidation (LPO) level and decreased level of GSH, SOD and CAT in SICH, ICG and LRG cell lines after exposure to increasing Ag-NPs in a concentration-dependent manner. This work proves that fish cell lines could be used as an alternative to whole animals using cytotoxicity tests, genotoxicity tests and oxidative stress assessment after exposure to nanoparticles.
authors: OIE Reference Laboratory for WTD, Aquaculture Biotechnology Laboratory, PG and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam, Tamil Nadu 632 509, India.
authors: G
authors: G
authors: Taju
authors: OIE Reference Laboratory for WTD, Aquaculture Biotechnology Laboratory, PG and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam, Tamil Nadu 632 509, India.
authors: S
authors: S
authors: Abdul Majeed
authors: OIE Reference Laboratory for WTD, Aquaculture Biotechnology Laboratory, PG and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam, Tamil Nadu 632 509, India.
authors: K S N
authors: KS
authors: Nambi
authors: OIE Reference Laboratory for WTD, Aquaculture Biotechnology Laboratory, PG and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam, Tamil Nadu 632 509, India. Electronic address: cah_sahul@hotmail.com.
authors: A S
authors: AS
authors: Sahul Hameed
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001681:Biological Assay / Q000379:methods; D002460:Cell Line; D020552:Comet Assay / Q000379:methods; D004249:DNA Damage / Q000187:drug effects; D005399:Fishes / Q000378:metabolism*; D005880:Gills / Q000187:drug effects* / Q000378:metabolism; D006321:Heart / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects; D053768:Metal Nanoparticles / Q000633:toxicity*; D009152:Mutagenicity Tests / Q000379:methods; D018384:Oxidative Stress / Q000187:drug effects; D012834:Silver / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D000975:Antioxidants; D014874:Water Pollutants, Chemical; D012834:Silver
keywords: Cytotoxicity; Fish cell lines; Genotoxicity; Linear correlation; Oxidative stress; Silver nanoparticles