title: TRAIL-engineered bone marrow-derived mesenchymal stem cells: TRAIL expression and cytotoxic effects on C6 glioma cells.
abstract: BACKGROUND
  TNF-related apoptosis-inducing ligand (TRAIL) is considered as a tumor cell-specific cytotoxic agent. Through the aid of mesenchymal stem cells (MSCs), TRAIL is capable of inducing apoptosis of tumor cells in tumor sites. The present study was performed to investigate the cytotoxic effects of TRAIL-engineered MSCs on glioblastoma cells (C6) in vitro.
  
  
  METHODS
  An expression vector of secreting form of TRAIL was used to engineer MSCs. The cytotoxic effects of TRAIL-transfected MSCs on C6 cells were invstigated using the MTT method and Hochest33258 staining after co-culture of the two cell types.
  
  
  RESULTS
  TRAIL and control plasmid transfection of MSCs showed no significant effect on MSC's viability (p>0.05). A significant inhibition of C6 cells was observed when they were co-cultured with TRAIL-engineered MSCs (63.7%±0.12, p<0.05).
  
  
  CONCLUSIONS
  Mesenchymal stem cells were very well tolerant to the transfection of TRAIL-bearing vectors. The cytotoxic effects of TRAIL-engineered MSCs on C6 cells indicates the therapeutic potential of this strategy for treatment of glioblastoma patients.
authors: University of British Columbia, 400-828 West 10th Avenue, Vancouver, BC, V5Z 1L8 Canada. Tel: +1 6048754111 ext. 62501, ljdai@mail.ubc.ca or longjundai@gmail.com.
authors: Xiang-Jun
authors: XJ
authors: Tang
authors: Jun-Ti
authors: JT
authors: Lu
authors: Han-Jun
authors: HJ
authors: Tu
authors: Kuan-Ming
authors: KM
authors: Huang
authors: Rui
authors: R
authors: Fu
authors: Gang
authors: G
authors: Cao
authors: Min
authors: M
authors: Huang
authors: Long-Hai
authors: LH
authors: Cheng
authors: Long-Jun
authors: LJ
authors: Dai
authors: Li
authors: L
authors: Zhang
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology / Q000628:therapy*; D018920:Coculture Techniques; D005818:Genetic Engineering / Q000379:methods; D005909:Glioblastoma / Q000473:pathology / Q000628:therapy*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D055647:Rats, Transgenic; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000096:biosynthesis / Q000235:genetics*; D014162:Transfection
chemical_list: D053221:TNF-Related Apoptosis-Inducing Ligand; C505655:Tnfsf10 protein, rat
keywords: Cytotherapy; gene therapy; glioblastoma; mesenchymal stem cells