title: A novel facility for 3D micro-irradiation of living cells in a controlled environment by MeV ions.
abstract: We present a novel facility for micro-irradiation of living targets with ions from a 1.7 MV tandem accelerator. We show results using 1 MeV protons and 2 MeV He(2+). In contrast to common micro-irradiation facilities, which use electromagnetic or electrostatic focusing and specially designed vacuum windows, we employ a tapered glass capillary with a thin end window, made from polystyrene with a thickness of 1-2 μm, for ion focusing and extraction. The capillary is connected to a beamline tilted vertically by 45°, which allows for easy immersion of the extracted ions into liquid environment within a standard cell culture dish. An inverted microscope is used for simultaneously observing the samples as well as the capillary tip, while a stage-top incubator provides an appropriate environment for the samples. Furthermore, our setup allows to target volumes in cells within a μm(3) resolution, while monitoring the target in real time during and after irradiation.
authors: Atomic Physics Laboratory, RIKEN, 351-0198 Wako-shi, Saitama, Japan.
authors: V
authors: V
authors: Mäckel
authors: Atomic Physics Laboratory, RIKEN, 351-0198 Wako-shi, Saitama, Japan.
authors: W
authors: W
authors: Meissl
authors: Atomic Physics Laboratory, RIKEN, 351-0198 Wako-shi, Saitama, Japan.
authors: T
authors: T
authors: Ikeda
authors: Cellular Dynamics Laboratory, RIKEN, 351-0198 Wako-shi, Saitama, Japan.
authors: M
authors: M
authors: Clever
authors: Atomic Physics Laboratory, RIKEN, 351-0198 Wako-shi, Saitama, Japan.
authors: E
authors: E
authors: Meissl
authors: Atomic Physics Laboratory, RIKEN, 351-0198 Wako-shi, Saitama, Japan.
authors: T
authors: T
authors: Kobayashi
authors: Atomic Physics Laboratory, RIKEN, 351-0198 Wako-shi, Saitama, Japan.
authors: T M
authors: TM
authors: Kojima
authors: Cellular Dynamics Laboratory, RIKEN, 351-0198 Wako-shi, Saitama, Japan.
authors: N
authors: N
authors: Imamoto
authors: Atomic Physics Laboratory, RIKEN, 351-0198 Wako-shi, Saitama, Japan.
authors: K
authors: K
authors: Ogiwara
authors: Atomic Physics Laboratory, RIKEN, 351-0198 Wako-shi, Saitama, Japan.
authors: Y
authors: Y
authors: Yamazaki
mesh_terms: D002470:Cell Survival; D004780:Environment, Controlled; D004867:Equipment Design; D006367:HeLa Cells; D006801:Humans; D055616:Microtechnology / Q000295:instrumentation*; D010315:Particle Accelerators / Q000295:instrumentation*; D011522:Protons
chemical_list: D011522:Protons