title: Optimization of selenylation conditions for lycium barbarum polysaccharide based on antioxidant activity.
abstract: Lycium barbarum polysaccharide (LBP) was modified by HNO3-Na2SeO3 method according to L9(3(4)) orthogonal design to obtain nine selenizing LBPs (sLBPs), sLBP1-sLBP9. Their antioxidant activities in vitro were compared by free radical-scavenging test. sLBP6, sLBP8 and sLBP9 presented stronger activity. In vivo test, 14-day-old chickens were injected respectively with sLBP6, sLBP8 and sLBP9 taking LBP as control, and serum GSH-Px and SOD activities and MDA content were determined. The results showed that three sLBPs could significantly enhance GSH-Px and SOD activities and decrease MDA content. The actions of sLBPs were significantly stronger than that of unmodified LBP. These results indicated that selenylation modification could significantly enhance the antioxidant activities of LBP, sLBP6 possessed the best efficacy and could be exploited into an antioxidant. The optimal modification conditions were 400mg of sodium selenite for 500 mg of LBP, reaction temperature of 70 °C and reaction time of 6h.
authors: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors: Shulei
authors: S
authors: Qiu
authors: National Research Center of Veterinary Biological Engineering and technology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China.
authors: Jin
authors: J
authors: Chen
authors: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors: Xi
authors: X
authors: Chen
authors: China Institute of Veterinary Drug Control, Beijing 100081, PR China.
authors: Qiang
authors: Q
authors: Fan
authors: China Institute of Veterinary Drug Control, Beijing 100081, PR China.
authors: Cunshuai
authors: C
authors: Zhang
authors: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors: Deyun
authors: D
authors: Wang
authors: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors: Xiuping
authors: X
authors: Li
authors: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors: Xingying
authors: X
authors: Chen
authors: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors: Xiaolan
authors: X
authors: Chen
authors: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors: Cui
authors: C
authors: Liu
authors: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors: Zhenzhen
authors: Z
authors: Gao
authors: College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Shanxi, Taigu 030801, PR China.
authors: Hongquan
authors: H
authors: Li
authors: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: ylhu@njau.edu.cn.
authors: Yuanliang
authors: Y
authors: Hu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology*; D002645:Chickens; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D032305:Lycium / Q000737:chemistry; D017942:Nitric Acid / Q000737:chemistry; D018038:Sodium Selenite / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; C500223:lycium barbarum polysaccharide; D017942:Nitric Acid; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D018038:Sodium Selenite
keywords: Free radical-scavenging rate; GSH-Px activity; MDA content; SOD activity; Selenizing lycium barbarum polysaccharide