title: Inhibition of Aurora-B suppresses osteosarcoma cell migration and invasion.
abstract: Previous studies have suggested that Aurora-B may be involved in cancer metastasis. However, its role has been poorly evaluated in osteosarcoma (OS). The aim of this study was to investigate the correlation between Aurora-B expression and metastasis in human OS. The human OS cell line, U2-OS, and OS biopsy specimens were used in the study. The expression of Aurora-B protein was examined using immunohistochemistry and western blotting in OS tissues and U2-OS cells, respectively. AZD1152-hydroxyquinazoline-pyrazol-anilide, an inhibitor of Aurora-B, was used to inhibit Aurora-B expression in U2-OS cells. The effect of Aurora-B inhibition on U2-OS cell proliferation, invasion and migration was assessed using MTT, colony formation, wound healing and Transwell assays. The results showed that positive expression of the Aurora-B protein was observed in the nucleus, and that Aurora-B expression levels in the cases with pulmonary metastases were significantly higher than in those without metastasis.In vitro, the proliferation, invasion and migration of U2-OS cells were suppressed by the inhibition of Aurora-B. These results suggest that Aurora-B may be involved in OS metastasis, and may be a promising target in the treatment of OS metastasis.
authors: Department of Anesthesia, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
authors: Xiao Ping
authors: XP
authors: Zhu
authors: Department of Orthopedics, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
authors: Zhi Li
authors: ZL
authors: Liu
authors: Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330004, P.R. China.
authors: Ai Fen
authors: AF
authors: Peng
authors: Department of Orthopedics, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
authors: Yun Fei
authors: YF
authors: Zhou
authors: Department of Orthopedics, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
authors: Xin Hua
authors: XH
authors: Long
authors: Department of Pathology, Cancer Hospital of Jiangxi Province, Nanchang, Jiangxi 330029, P.R. China.
authors: Qing Feng
authors: QF
authors: Luo
authors: Department of Orthopedics, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
authors: Shan Hu
authors: SH
authors: Huang
authors: Department of Orthopedics, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
authors: Yong
authors: Y
authors: Shu
keywords: AZD1152; Aurora-B; metastasis; osteosarcoma