title: Purification of vitronectin.
abstract: This unit describes the purification of extracellular vitronectin from plasma or serum by using heparin-affinity chromatography. First, the plasma is depleted of fibronectin plus other heparin- and Sepharose-binding proteins and treated with urea to activate the heparin-binding activity of vitronectin, which is subsequently bound to a heparin affinity column and eluted. The resulting vitronectin should be ∼ 98% pure.
authors: National Institute of Environmental Health Sciences, NIH, Research Triangle Park, North Carolina.
authors: Steven K
authors: SK
authors: Akiyama
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D006493:Heparin / Q000737:chemistry; D006801:Humans; D010949:Plasma / Q000737:chemistry; D012685:Sepharose / Q000737:chemistry; D044967:Serum / Q000737:chemistry; D019096:Vitronectin / Q000097:blood / Q000302:isolation & purification*
chemical_list: D019096:Vitronectin; D006493:Heparin; D012685:Sepharose
keywords: adhesion; affinity purification; cell biology; extracellular matrix