title: Lung development alterations in newborn mice after recovery from exposure to sublethal hyperoxia.
abstract: Exposure of newborn mice to hyperoxia arrests lung development, with resultant pathological characteristics similar to bronchopulmonary dysplasia in infants born prematurely. We tested the hypothesis that aberrations in lung development caused by 14 days of sublethal hyperoxia would be reversed during 14 days of recovery to room air (RA) when the concentration of oxygen exposure was weaned gradually. Newborn FVB mice were exposed to 85% oxygen or RA for 14 days. Weaning from hyperoxia was by either transfer directly into RA or a decrease in the concentration of oxygen by 10% per days. At 28 days, pups were euthanized, and the lungs were inflation fixed and assessed. At postnatal day 28, lungs of mice weaned abruptly from hyperoxia had fewer (6 ± 0.6 versus 10 ± 0.7; P < 0.001) alveoli per high-powered field and larger alveoli (4050 ± 207 versus 2305 ± 182 μm(2)) than animals weaned gradually; both hyperoxia-exposed groups were different from lungs obtained from air-breathing controls (20 ± 0.5 alveoli per high-powered field; P < 0.001). The results are consistent with the absence of catch-up alveolarization in this model and indicate that the long-term consequences of early exposures to hyperoxia merit closer examination. The effects of abrupt weaning to RA observed further suggest that weaning should be considered in experimental models of newborn exposure to hyperoxia.
authors: Section of Neonatology, Department of Pediatrics, Center for Perinatal Research, Nationwide Children's Hospital Research Institute, Columbus, Ohio; Neonatology and Pediatric Critical Care, Department of General Pediatrics, University Children's Hospital of Muenster, Muenster, Germany.
authors: Esther
authors: E
authors: Rieger-Fackeldey
authors: Section of Neonatology, Department of Pediatrics, Center for Perinatal Research, Nationwide Children's Hospital Research Institute, Columbus, Ohio; Department of Pediatrics, Yonsei University College of Medicine, Seoul, Republic of Korea.
authors: Min S
authors: MS
authors: Park
authors: Section of Neonatology, Department of Pediatrics, Center for Perinatal Research, Nationwide Children's Hospital Research Institute, Columbus, Ohio.
authors: Brandon L
authors: BL
authors: Schanbacher
authors: Section of Neonatology, Department of Pediatrics, Center for Perinatal Research, Nationwide Children's Hospital Research Institute, Columbus, Ohio.
authors: Mandar S
authors: MS
authors: Joshi
authors: Section of Neonatology, Department of Pediatrics, Center for Perinatal Research, Nationwide Children's Hospital Research Institute, Columbus, Ohio.
authors: Louis G
authors: LG
authors: Chicoine
authors: Section of Neonatology, Department of Pediatrics, Center for Perinatal Research, Nationwide Children's Hospital Research Institute, Columbus, Ohio.
authors: Leif D
authors: LD
authors: Nelin
authors: Section of Neonatology, Department of Pediatrics, Center for Perinatal Research, Nationwide Children's Hospital Research Institute, Columbus, Ohio.
authors: John A
authors: JA
authors: Bauer
authors: Section of Neonatology, Department of Pediatrics, Center for Perinatal Research, Nationwide Children's Hospital Research Institute, Columbus, Ohio. Electronic address: welty@bcm.edu.
authors: Stephen E
authors: SE
authors: Welty
authors: Section of Neonatology, Department of Pediatrics, Center for Perinatal Research, Nationwide Children's Hospital Research Institute, Columbus, Ohio; Center for Developmental Therapeutics, Seattle Children's Hospital Research Institute, Seattle, Washington.
authors: Charles V
authors: CV
authors: Smith
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001997:Bronchopulmonary Dysplasia / Q000209:etiology* / Q000473:pathology*; D004195:Disease Models, Animal; D018496:Hyperoxia / Q000150:complications*; D008168:Lung / Q000473:pathology*; D051379:Mice; D012121:Respiration, Artificial / Q000379:methods*