title: Coumestrol treatment prevents Na+, K+ -ATPase inhibition and affords histological neuroprotection to male rats receiving cerebral global ischemia.
abstract: OBJECTIVE
  In this study, we investigated the possible mechanisms underlying the neuroprotective effects of coumestrol, a potent isoflavonoid with antioxidant activities and binding affinities for both estrogen receptors (ER) ER-alpha and ER-beta that are comparable to those of 17beta-estradiol, in a model of global ischemia in male subjects.
  
  
  METHODS
  Wistar rats underwent global ischemia (10 minutes) or sham surgery and received a single intracerebroventricular (icv) infusion of 20 μg of coumestrol or vehicle 1 hour before ischemia or 0, 3, 6, or 24 hours after reperfusion.
  
  
  RESULTS
  The data analysis revealed an extensive neuronal death in the CA1 hippocampal subfield at 7 days, and a significant decrease in the Na+, K+ -ATPase activity at 1 and 24 hours after ischemia, and both injuries were attenuated by coumestrol administration.
  
  
  CONCLUSIONS
  Coumestrol treatment was effective in preventing neuronal loss in all times of administration as well as able to rescue the Na+, K+ -ATPase activity, suggesting its potential benefits for either prevention or therapeutics use against cerebral ischemia in males.
authors: Cibele Canal
authors: CC
authors: Castro
authors: Aline S
authors: AS
authors: Pagnussat
authors: Nathalia
authors: N
authors: Moura
authors: Maira J
authors: MJ
authors: da Cunha
authors: Fernanda R
authors: FR
authors: Machado
authors: Angela T S
authors: AT
authors: Wyse
authors: Carlos Alexandre
authors: CA
authors: Netto
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D056547:CA1 Region, Hippocampal / Q000187:drug effects* / Q000473:pathology; D016923:Cell Death; D003375:Coumestrol / Q000627:therapeutic use*; D008297:Male; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D017966:Pyramidal Cells / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
chemical_list: D018696:Neuroprotective Agents; D000254:Sodium-Potassium-Exchanging ATPase; D003375:Coumestrol
keywords: Cerebral ischemia,; Coumestrol,; Hippocampus; K+-ATPase,; Na+,; Neuroprotection,