title: Purification of synthetic peptides using a catching full-length sequence by polymerization approach.
abstract: During automated solid-phase peptide synthesis, failure sequences were capped with acetic anhydride. After synthesis, a polymerizable methacrylamide tag was attached to the full-length sequences. Peptide purification was then achieved by polymerizing the full-length sequences, washing away impurities, and cleaving the peptide product from the polymer.
authors: Department of Chemistry, Michigan Technological University , 1400 Townsend Drive, Houghton, Michigan 49931 United States.
authors: Mingcui
authors: M
authors: Zhang
authors: Durga
authors: D
authors: Pokharel
authors: Shiyue
authors: S
authors: Fang
mesh_terms: D000089:Acetic Anhydrides; D000178:Acrylamides / Q000737:chemistry; D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D015394:Molecular Structure; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D058105:Polymerization; D060327:Solid-Phase Synthesis Techniques
chemical_list: D000089:Acetic Anhydrides; D000178:Acrylamides; D010455:Peptides; C031800:acetic anhydride; C045985:methacrylamide