title: Arabidopsis ABCG14 protein controls the acropetal translocation of root-synthesized cytokinins.
abstract: Cytokinins are a major group of phytohormones regulating plant growth, development and stress responses. However, in contrast to the well-defined polar transport of auxins, the molecular basis of cytokinin transport is poorly understood. Here we show that an ATP-binding cassette transporter in Arabidopsis, AtABCG14, is essential for the acropetal (root to shoot) translocation of the root-synthesized cytokinins. AtABCG14 is expressed primarily in the pericycle and stelar cells of roots. Knocking out AtABCG14 strongly impairs the translocation of trans-zeatin (tZ)-type cytokinins from roots to shoots, thereby affecting the plant's growth and development. AtABCG14 localizes to the plasma membrane of transformed cells. In planta feeding of C(14) or C(13)-labelled tZ suggests that it acts as an efflux pump and its presence in the cells directly correlates with the transport of the fed cytokinin. Therefore, AtABCG14 is a transporter likely involved in the long-distance translocation of cytokinins in planta.
authors: 1] Biosciences Department, Brookhaven National Laboratory, Upton, New York 11973, USA [2].
authors: Kewei
authors: K
authors: Zhang
authors: Laboratory of Growth Regulators, Faculty of Science, Palacký University & Institute of Experimental Botany AS CR, Olomouc CZ-78371, Czech Republic.
authors: Ondrej
authors: O
authors: Novak
authors: Biosciences Department, Brookhaven National Laboratory, Upton, New York 11973, USA.
authors: Zhaoyang
authors: Z
authors: Wei
authors: Biosciences Department, Brookhaven National Laboratory, Upton, New York 11973, USA.
authors: Mingyue
authors: M
authors: Gou
authors: Biosciences Department, Brookhaven National Laboratory, Upton, New York 11973, USA.
authors: Xuebin
authors: X
authors: Zhang
authors: Department of Biological Sciences, St. John's University, Queens, New York 11439, USA.
authors: Yong
authors: Y
authors: Yu
authors: Biosciences Department, Brookhaven National Laboratory, Upton, New York 11973, USA.
authors: Huijun
authors: H
authors: Yang
authors: Biosciences Department, Brookhaven National Laboratory, Upton, New York 11973, USA.
authors: Yuanheng
authors: Y
authors: Cai
authors: 1] Laboratory of Growth Regulators, Faculty of Science, Palacký University & Institute of Experimental Botany AS CR, Olomouc CZ-78371, Czech Republic [2] Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University, CZ-783 71 Olomouc, Czech Republic.
authors: Miroslav
authors: M
authors: Strnad
authors: Biosciences Department, Brookhaven National Laboratory, Upton, New York 11973, USA.
authors: Chang-Jun
authors: CJ
authors: Liu
mesh_terms: D000070996:ATP Binding Cassette Transporter, Subfamily G; D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D017360:Arabidopsis; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001692:Biological Transport; D002247:Carbon Isotopes; D002250:Carbon Radioisotopes; D003583:Cytokinins / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D018520:Plant Shoots / Q000378:metabolism*; D015026:Zeatin / Q000378:metabolism
chemical_list: C000589386:ABCG14 protein, Arabidopsis; D000070996:ATP Binding Cassette Transporter, Subfamily G; D018528:ATP-Binding Cassette Transporters; D029681:Arabidopsis Proteins; D002247:Carbon Isotopes; D002250:Carbon Radioisotopes; D003583:Cytokinins; D015026:Zeatin