title: Antiproliferative activity and induction of apoptosis in PC-3 cells by the chalcone cardamonin from Campomanesia adamantium (Myrtaceae) in a bioactivity-guided study.
abstract: The Myrtaceae family is a common source of medicines used in the treatment of numerous diseases in South America. In Brazil, fruits of the Campomanesia species are widely used to make liqueurs, juices and sweets, whereas leaves are traditionally employed as a medicine for dysentery, stomach problems, diarrhea, cystitis and urethritis. Ethanol extracts of Campomanesia adamantium (Myrtaceae) leaves and fruits were evaluated against prostate cancer cells (PC-3). The compound (2E)-1-(2,4-dihydroxy-6-methoxyphenyl)-3-phenylprop-2-en-1-one, cardamonin) was isolated from ethanol extracts of C. adamantium leaves in a bioactivity-guided study and quantified by UPLC-MS/MS. In vitro studies showed that the isolated chalcone cardamonin inhibited prostate cancer cell proliferation and decreased the expression of NFkB1. Moreover, analysis by flow cytometry showed that this compound induced DNA fragmentation, suggesting an effect on apoptosis induction in the PC-3 cell line.
authors: Graduate Program in Biosciences and Technology of Bioactive Products, Pharmacy course, State University of Campinas, Campinas, 6109, São Paulo 13083-970, Brazil. aislanfagundes@yahoo.com.br.
authors: Aislan Cristina Rheder Fagundes
authors: AC
authors: Pascoal
authors: Department of Chemistry, Federal University of Paraná, 19081, Curitiba 81531-990, Paraná, Brazil. slash@ufpr.br.
authors: Carlos Augusto
authors: CA
authors: Ehrenfried
authors: Graduate Program in Biosciences and Technology of Bioactive Products, Pharmacy course, State University of Campinas, Campinas, 6109, São Paulo 13083-970, Brazil. begogcl@gmail.com.
authors: Begoña Gimenez-Cassina
authors: BG
authors: Lopez
authors: Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas, Campinas 13083-887, São Paulo, Brazil. thiagomatosaraujo@gmail.com.
authors: Thiago Matos
authors: TM
authors: de Araujo
authors: Faculty of Basic Sciences, Fluminense Federal University, Nova Friburgo 24020-420, Rio de Janeiro, Brazil. vinicius_pascoal@yahoo.com.br.
authors: Vinicius D'ávila Bitencourt
authors: VD
authors: Pascoal
authors: Multidisciplinary Center for Biological Research, Laboratory Animal Quality Control, State University of Campinas, l6095, Campinas 13083-877, São Paulo, Brazil. rovilson@unicamp.br.
authors: Rovilson
authors: R
authors: Gilioli
authors: Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas, Campinas 13083-887, São Paulo, Brazil. anhegf@yahoo.com.br.
authors: Gabriel Forato
authors: GF
authors: Anhê
authors: Division of Pharmacology and Toxicology, Multidisciplinary Center for Chemical, Biological and Agricultural, State University of Campinas, 6171, Campinas 13081-970, São Paulo, Brazil. aa_ruiz@hotmail.com.
authors: Ana Lúcia Tasca Goes
authors: AL
authors: Ruiz
authors: Division of Pharmacology and Toxicology, Multidisciplinary Center for Chemical, Biological and Agricultural, State University of Campinas, 6171, Campinas 13081-970, São Paulo, Brazil. carvalho_je@yahoo.com.br.
authors: João Ernesto de
authors: JE
authors: Carvalho
authors: Department of Chemistry, Federal University of Paraná, 19081, Curitiba 81531-990, Paraná, Brazil. elida@ufpr.br.
authors: Maria Elida Alves
authors: ME
authors: Stefanello
authors: Graduate Program in Biosciences and Technology of Bioactive Products, Pharmacy course, State University of Campinas, Campinas, 6109, São Paulo 13083-970, Brazil. marcosjs@unicamp.br.
authors: Marcos José
authors: MJ
authors: Salvador
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001938:Brazil; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002599:Chalcone / Q000737:chemistry / Q000494:pharmacology; D047188:Chalcones / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008297:Male; D027822:Myrtaceae / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000473:pathology
chemical_list: D047188:Chalcones; D010936:Plant Extracts; D002599:Chalcone; C436747:cardamonin