title: Differential Nrf2 expression between glioma stem cells and non-stem-like cells in glioblastoma.
abstract: Glioblastoma multiforme (GBM), the most commonly occurring primary intracranial tumor, is associated with a negative outcome, regardless of the availability of multimodal therapies. However, the identification of glioma stem cells (GSCs), which are small groups of cells within the GBM, has resulted in novel avenues for research. GSCs are resistant to numerous types of environmental stress, such as irradiation, antitumor drugs and hypoxia. Nuclear factor erythroid 2-related factor 2 (Nrf2) has a significant role the cellular response to oxidative stress and previous studies have supported the significance of Nrf2 in GBM; however, the role of Nrf2 in GSCs remains unclear. In the present study, Nrf2 in CD133-GBM cells and CD133+GSCs from GBM were compared. GSCs from GBM, which express the surface marker CD133, were separated by magnetic cell sorting and analyzed by immunofluorescence in 24-well clusters and cell counting using flow cytometry. The expression of Nrf2 was detected at the transcriptional and translational levels in CD133+and CD133-cells, and the result indicated that GSCs were successfully isolated from the GBM. The percentage of tumor stem cells in total cells was between 0.49 and 0.91%. Nrf2 was overexpressed in CD133+GSCs when compared with CD133-GBM cells, which indicated that the expression of Nrf2 in GSCs was closely correlated with malignant proliferation and differentiation of the GBM. Therefore, it was concluded that Nrf2 may be a potential biomarker and rational therapeutic target in GBM.
authors: Department of Neurosurgery, Jinling Hospital Affiliated to Nanjing University School of Medicine, Nanjing, Jiangsu 210089, P.R. China.
authors: Jianhong
authors: J
authors: Zhu
authors: Department of Neurosurgery, Jinling Hospital, Neurosurgical Institution of People's Liberation Army of China, Nanjing, Jiangsu 210002, P.R. China.
authors: Handong
authors: H
authors: Wang
authors: Department of Neurosurgery, Jinling Hospital Affiliated to Nanjing University School of Medicine, Nanjing, Jiangsu 210089, P.R. China.
authors: Xiangjun
authors: X
authors: Ji
authors: Department of Neurosurgery, Jinling Hospital, Neurosurgical Institution of People's Liberation Army of China, Nanjing, Jiangsu 210002, P.R. China.
authors: Lin
authors: L
authors: Zhu
authors: Department of Neurosurgery, Jinling Hospital Affiliated to Nanjing University School of Medicine, Nanjing, Jiangsu 210089, P.R. China.
authors: Qing
authors: Q
authors: Sun
authors: Department of Neurosurgery, Jinling Hospital Affiliated to Nanjing University School of Medicine, Nanjing, Jiangsu 210089, P.R. China.
authors: Zixiang
authors: Z
authors: Cong
authors: Department of Neurosurgery, Jinling Hospital, Neurosurgical Institution of People's Liberation Army of China, Nanjing, Jiangsu 210002, P.R. China.
authors: Yuan
authors: Y
authors: Zhou
authors: Neurosurgery Department of Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
authors: Huandong
authors: H
authors: Liu
authors: Department of Neurosurgery, Jinling Hospital, Neurosurgical Institution of People's Liberation Army of China, Nanjing, Jiangsu 210002, P.R. China.
authors: Mengliang
authors: M
authors: Zhou
keywords: glioblastoma; glioma stem cell; nuclear factor erythroid 2-related factor 2