title: Gene Expression Profiles Underlying Selective T-Cell-Mediated Immunity Activity of a Chinese Medicine Granule on Mice Infected with Influenza Virus H1N1.
abstract: A Chinese medicine granule, Shu-Feng-Xuan-Fei (SFXF), is critical for viral clearance in early phase of influenza virus infection. In this study, 72 ICR mice were randomly divided into six groups: normal control group, virus control group, Oseltamivir group, low-dose SFXF, medium-dose SFXF, and high-dose SFXF. Mice were anesthetized and inoculated with 4LD50 of influenza virus A (H1N1) except normal control group. Oseltamivir group received 11.375 mg·kg(-1) ·d(-1) Oseltamivir Phosphate. SFXF 3.76, 1.88 and 0.94 g·kg(-1) ·d(-1) were administrated to mice in all SFXF groups. Each group was in equal dose of 0.2ml daily for 4 consecutive days. Mice were sacrificed and then total RNA was extracted in lung tissue. Some genes involved in T-cell-mediated immunity were selected by DNA microarray. These candidate genes were verified by Real-Time PCR and western immunoblotting. Compared with virus control group, in Toll-like receptor signaling pathway, 12 virus-altered genes were significantly reduced following medium-dose SFXF treatment. Eighteen antigen processing presentation-associated genes were upregulated by medium-dose SFXF. In the process of T cell receptor signaling pathway, 19 genes were downregulated by medium-dose SFXF treatment. On exploration into effector T cells activation and cytokines, all of altered genes in virus control group were reversed by medium-dose SFXF. Real-time PCR and western immunoblotting showed that the regulation of medium-dose SFXF in IL-4, IFN-γ, TNF-α, IL-1β, TLR7, MyD88, p38, and JNK was superior to Oseltamivir and high-dose SFXF group. Therefore, SFXF granules could reduce influenza infected cells and activation of T cells.
authors: Laboratory of Chinese Medicine on Viral Disease, Basic Medical College, Beijing University of Chinese Medicine, Beijing 100029, China.
authors: Na-Na
authors: NN
authors: Lu
authors: Laboratory of Chinese Medicine on Viral Disease, Basic Medical College, Beijing University of Chinese Medicine, Beijing 100029, China ; Shanxi University of Traditional Chinese Medicine, Taiyuan, Shanxi 030024, China.
authors: Qi
authors: Q
authors: Liu
authors: Laboratory of Chinese Medicine on Viral Disease, Basic Medical College, Beijing University of Chinese Medicine, Beijing 100029, China.
authors: Li-Gang
authors: LG
authors: Gu
authors: Laboratory of Chinese Medicine on Viral Disease, Basic Medical College, Beijing University of Chinese Medicine, Beijing 100029, China.
authors: Shi-Jie
authors: SJ
authors: Ge
authors: Laboratory of Chinese Medicine on Viral Disease, Basic Medical College, Beijing University of Chinese Medicine, Beijing 100029, China.
authors: Jun
authors: J
authors: Wu
authors: Laboratory of Chinese Medicine on Viral Disease, Basic Medical College, Beijing University of Chinese Medicine, Beijing 100029, China.
authors: Qiu
authors: Q
authors: Ze-Ji
authors: Department of Respiratory Medicine, China-Japan Friendship Hospital, Beijing 100029, China.
authors: Ze-Ji
authors: ZJ
authors: Qiu
authors: Department of Respiratory Medicine, China-Japan Friendship Hospital, Beijing 100029, China.
authors: Hong-Chun
authors: HC
authors: Zhang
authors: Laboratory of Chinese Medicine on Viral Disease, Basic Medical College, Beijing University of Chinese Medicine, Beijing 100029, China.
authors: En-Xiang
authors: EX
authors: Chao
authors: Laboratory of Chinese Medicine on Viral Disease, Basic Medical College, Beijing University of Chinese Medicine, Beijing 100029, China.
authors: Zhuo-Nan
authors: ZN
authors: Yu