title: The effects of diosgenin in the regulation of renal proximal tubular fibrosis.
abstract: Fibrosis is the important pathway for end-stage renal failure. Glucose has been demonstrated to be the most important fibrogenesis-inducing agent according to previous studies. Despite diosgenin has been demonstrated to be anti-inflammatory, the possible role in fibrosis regulation of diosgenin remain to be investigated. In this study, renal proximal tubular epithelial cells (designated as HK-2) were treated with high concentration of glucose (HG, 27.5mM) to determine whether diosgenin (0.1, 1 and 10 μM) has the effects to regulate renal cellular fibrosis. We found that 10 μM of diosgenin exert optimal inhibitory effects on high glucose-induced fibronectin expression in HK-2 cells. In addition, diosgenin markedly inhibited HG-induced increase in α-smooth muscle actin (α-SMA) and HG-induced decrease in E-cadherin. In addition, diosgenin antagonizes high glucose-induced epithelial-to-mesenchymal transition (EMT) signals partly by enhancing the catabolism of Snail in renal cells. Collectively, these data suggest that diosgenin has the potential to inhibit high glucose-induced renal tubular fibrosis possibly through EMT pathway.
authors: Graduate Institute of Biomedical Science, Chung Hwa University of Medical Technology, Tainan, Taiwan.
authors: Wei-Cheng
authors: WC
authors: Wang
authors: Graduate Institute of Biomedical Science, Chung Hwa University of Medical Technology, Tainan, Taiwan; Department of Internal Medicine, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung, Taiwan.
authors: Shu-Fen
authors: SF
authors: Liu
authors: Graduate Institute of Biomedical Science, Chung Hwa University of Medical Technology, Tainan, Taiwan.
authors: Wen-Teng
authors: WT
authors: Chang
authors: Graduate Institute of Biomedical Science, National Sun Yat-sen University, Kaohsiung, Taiwan.
authors: Yow-Ling
authors: YL
authors: Shiue
authors: Graduate Institute of Biomedical Science, National Sun Yat-sen University, Kaohsiung, Taiwan.
authors: Pei-Fang
authors: PF
authors: Hsieh
authors: Graduate Institute of Biomedical Science, Chung Hwa University of Medical Technology, Tainan, Taiwan.
authors: Tsung-Jen
authors: TJ
authors: Hung
authors: Department of Food nutrition, Chung Hwa University of Medical Technology, Tainan, Taiwan.
authors: Chien-Ya
authors: CY
authors: Hung
authors: Graduate Institute of Biomedical Science, Chung Hwa University of Medical Technology, Tainan, Taiwan; Department of Public Health, National Taiwan University, Taipei, Taiwan.
authors: Yu-Ju
authors: YJ
authors: Hung
authors: Graduate Institute of Biomedical Science, Chung Hwa University of Medical Technology, Tainan, Taiwan.
authors: Mei-Fen
authors: MF
authors: Chen
authors: Graduate Institute of Biomedical Science, Chung Hwa University of Medical Technology, Tainan, Taiwan; Department of Medical Laboratory Science and Biotechnology, Chung Hwa University of Medical Technology, Tainan, Taiwan. Electronic address: Call0955443221@gmail.com.
authors: Yu-Lin
authors: YL
authors: Yang
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D004144:Diosgenin / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism; D005355:Fibrosis / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D007687:Kidney Tubules, Proximal / Q000378:metabolism / Q000473:pathology*; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D000199:Actins; D015820:Cadherins; D005353:Fibronectins; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; D005947:Glucose; D004144:Diosgenin
keywords: Epithelial-to-mesenchymal transition; Glucose; Renal tubular fibrosis