title: In vitro evaluation of doxorubicin-incorporated magnetic albumin nanospheres.
abstract: Magnetic albumin nanospheres that incorporate doxorubicin (M-DOX-BSA-NPs) were prepared previously by our research group to develop magnetically responsive drug carrier system. This nanocarrier was synthesized as a drug delivery system for targeted chemotherapy. In this work, cytotoxic effects of doxorubicin (DOX)-loaded/unloaded or magnetic/non-magnetic nanoparticles and free DOX against PC-3 cells and A549 cells were determined with the MTT test and the results were compared with each other. DOX-loaded magnetic albumin nanospheres (M-DOX-BSA-NPs) were found more cytotoxic than other formulations. The quantitative data obtained from flow cytometry analysis further verified the higher targeting and killing ability of M-DOX-BSA-NPs than free DOX on both of the cancer cell lines. Additionally, the results of cell cycle analysis have showed that M-DOX-BSA-NPs affected G1 and G2 phases. Finally, cell images were obtained using spin-disk confocal microscopy, and cellular uptake of M-DOX-BSA-NPs was visualized. The findings of this study suggest that M-DOX-BSA-NPs represent a potential doxorubicin delivery system for targeted drug transport into prostate and lung cancer cells.
authors: Department of Chemistry, Faculty of Science, İzmir Institute of Technology, 35430, Urla, İzmir, Turkey.
authors: Ayça
authors: A
authors: Zeybek
authors: Gülşah
authors: G
authors: Şanlı-Mohamed
authors: Güliz
authors: G
authors: Ak
authors: Habibe
authors: H
authors: Yılmaz
authors: Şenay H
authors: ŞH
authors: Şanlıer
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage* / Q000494:pharmacology; D002417:Cattle; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000008:administration & dosage* / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D060328:Magnetic Phenomena; D059346:Magnets / Q000737:chemistry*; D054118:Nanospheres / Q000737:chemistry* / Q000378:metabolism; D009369:Neoplasms / Q000188:drug therapy; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000903:Antibiotics, Antineoplastic; D004337:Drug Carriers; D012710:Serum Albumin, Bovine; D004317:Doxorubicin
keywords: A549 cell line; PC3 cell line; apoptosis; doxorubicin; magnetic albumin nanoparticle; targeted drug delivery