title: Anti-allergic effect of a Korean traditional medicine, Biyeom-Tang on mast cells and allergic rhinitis.
abstract: BACKGROUND
  Biyeom-Tang, a medicine prescribed by oriental clinics, has been used for the treatment of the allergic rhinitis (AR). In the present study, an ethanol extract of Biyeom-Tang (EBT) was investigated for anti-allergic properties on bone-marrow derived mast cells (BMMC) and in vivo models.
  
  
  METHODS
  The anti-allergic properties of EBT were evaluated by measuring β-Hex release and the production of prostaglandin D2 (PGD2) and leukotriene C4 (LTC4) on BMMC in vitro and PCA and OVA-induced AR models in vivo.
  
  
  RESULTS
  EBT strongly inhibited a degranulation reaction in a dose dependent manner with an IC50 value of 35.6 μg/ml. In addition, the generation of PGD2 and LTC4 was inhibited in BMMC in a concentration-dependent manner with IC50 values of 7.0 μg/ml and 10.9 μg/ml, respectively. When administrated orally, EBT ameliorated the mast cell-mediated PCA reaction. In the OVA-induced AR model, the increased levels of IgE were reduced by EBT. The levels of cytokines, such as IL-4, IL-5, IL-10, and IL-13 decreased in the splenocytes of EBT-treated mice. The histological analysis shows that the infiltration of inflammatory cells increased by OVA-sensitization was also reduced.
  
  
  CONCLUSIONS
  Taken together, these results suggested that EBT has anti-allergic and anti-inflammatory effects in vitro and in vivo models.
authors: Kyu-Tae
authors: KT
authors: Jeong
authors: Sun-Gun
authors: SG
authors: Kim
authors: Jiean
authors: J
authors: Lee
authors: Young Na
authors: YN
authors: Park
authors: Hyo-Hyun
authors: HH
authors: Park
authors: Na-Young
authors: NY
authors: Park
authors: Keuk-Jun
authors: KJ
authors: Kim
authors: Hwadong
authors: H
authors: Lee
authors: Youn Ju
authors: YJ
authors: Lee
authors: Research and Development Division, Korea Promotion Institute for Traditional Medicine Industry, Gyeongsan 712-260, Republic of Korea. eklee@ynu.ac.kr.
authors: Eunkyung
authors: E
authors: Lee
mesh_terms: D029969:Angelica; D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use; D001854:Bone Marrow Cells / Q000187:drug effects; D004195:Disease Models, Animal; D005260:Female; D007073:Immunoglobulin E / Q000378:metabolism; D007378:Interleukins / Q000378:metabolism*; D008297:Male; D008407:Mast Cells / Q000187:drug effects*; D055670:Medicine, Korean Traditional; D027541:Mentha; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D015230:Prostaglandin D2 / Q000378:metabolism*; D065631:Rhinitis, Allergic / Q000188:drug therapy* / Q000378:metabolism; D029778:Trichosanthes; D031210:Xanthium
chemical_list: D018926:Anti-Allergic Agents; D000893:Anti-Inflammatory Agents; D007378:Interleukins; D010936:Plant Extracts; D007073:Immunoglobulin E; D015230:Prostaglandin D2