title: Association of 8q24.21 rs10505477-rs6983267 haplotype and age at diagnosis of colorectal cancer.
abstract: BACKGROUND
  Colorectal cancer (CRC) is the fourth most common cause of cancer death in the world. Genetic variants in 8q24.21 including rs10505477 and rs6983267 have been hypothesized to be involved in susceptibility to CRC. This study aims to investigate the possible association between these loci and their haplotypes with CRC risk in Iranian population.
  
  
  METHODS
  Subjects were recruited from two hospitals in Tehran. The rs10505477 and rs6983267 polymorphisms were genotyped by TaqMan real time PCR using subject genomic DNA, extracted either from formalin-fixed, paraffin-embedded tissue of patients or from blood of the controls by standard methods.
  
  
  RESULTS
  A total of 715 subjects (380 CRC patients and 335 matched controls) were genotyped in this study. Allele and genotype analysis of the rs10505477 and rs6983267 polymorphisms by gender, age at diagnosis, tumor location, tumor grade, and tumor node metastasis (TNM) showed no significant association with CRC risk. There was a significant relationship between GG haplotype and susceptibility to age at diagnosis for both <60 and ≥60 (p=0.0005 and p=0.000004, respectively) and between GT and CRC in the age at diagnosis ≥ 60 (Table 3: p=0.031). The GG haplotype was less frequent in CRC patients with the age at diagnosis <60, but was more common in subjects with the age at diagnosis ≥ 60.
  
  
  CONCLUSIONS
  Results of this study suggests that the rs6983267 and rs10505477 polymorphisms alone may not be relevant to CRC risk, but their GG haplotype plays a notable role in age at diagnosis of CRC in the Iranian population.
authors: Gastroenterology and Liver Diseases Research Center (GLDRC), Shahid Beheshti University of Medical Science, Tehran, Iran E-mail : nnzali@hotmail.com, batoolsadat@yahoo.com.
authors: Monir Sadat
authors: MS
authors: Haerian
authors: Batoul Sadat
authors: BS
authors: Haerian
authors: Hassan
authors: H
authors: Rooki
authors: Saadat
authors: S
authors: Molanaei
authors: Farid
authors: F
authors: Kosari
authors: Maedeh
authors: M
authors: Obohhat
authors: Parisa
authors: P
authors: Hosseinpour
authors: Pedram
authors: P
authors: Azimzadeh
authors: Seyed Reza
authors: SR
authors: Mohebbi
authors: Zahra
authors: Z
authors: Akbari
authors: Mohammad Reza
authors: MR
authors: Zali
mesh_terms: D000328:Adult; D000367:Age Factors; D017668:Age of Onset; D000368:Aged; D016022:Case-Control Studies; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D007492:Iran / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic