title: TNFα-induced miR-130 resulted in adipocyte dysfunction during obesity-related inflammation.
abstract: Adipocytes are continuously stimulated by proinflammatory cytokines such as TNFα, which cause adipocyte dysfunction by facilitating the inflammatory response. Although miR-130 was reported to be an important regulator of adipogenesis by targeting PPARγ mRNA, little is known about the mechanisms regulating miR-130 expression during the proinflammatory response. Here, we examined miR-130 levels in white adipose tissue (WAT) from high-fat diet (HFD) mice and TNFα-stimulated adipocytes. Primary transcripts of miR-130 were increased after TNFα stimulation, indicating that induction of miR-130 during the pro-inflammatory response is regulated by a transcriptional event. A chromatin immunoprecipitation assay showed that p65 binding to the promoter regions of miR-130 was enhanced after TNFα treatment. Taken together, our findings suggest that induction of miR-130 by TNFα is responsible for adipocyte dysfunction.
authors: Department of Biochemistry, College of Medicine, Catholic University of Korea, Seoul 137-701, South Korea.
authors: Chongtae
authors: C
authors: Kim
authors: Department of Biochemistry, College of Medicine, Catholic University of Korea, Seoul 137-701, South Korea.
authors: Heejin
authors: H
authors: Lee
authors: Department of Biochemistry, College of Medicine, Catholic University of Korea, Seoul 137-701, South Korea.
authors: Yoon Mi
authors: YM
authors: Cho
authors: Department of Biochemistry, College of Medicine, Catholic University of Korea, Seoul 137-701, South Korea.
authors: Oh-Joo
authors: OJ
authors: Kwon
authors: Department of Molecular Science and Technology, Ajou University, Suwon, 443-749 South Korea.
authors: Wook
authors: W
authors: Kim
authors: Department of Biochemistry, College of Medicine, Catholic University of Korea, Seoul 137-701, South Korea. Electronic address: leeek@catholic.ac.kr.
authors: Eun Kyung
authors: EK
authors: Lee
keywords: NFκB; PPARγ; adipocyte dysfunction; miR-130