title: Analysis of adipose tissue lipid using mass spectrometry.
abstract: Mass spectrometry technology has enabled significant advances in detailing the alterations of the lipidome in response to pathological conditions or experimental manipulations. Lipids comprise a wide range of compounds with functions that include structural, intracellular signaling, trafficking, and storage. Characterization of lipid species has evolved significantly over recent years due to the progress made in the area of mass spectrometry. This chapter details the methods used for the analysis of lipids tailored to the intrinsic characteristics of adipose tissue. Particular attention is given to the analysis of triglycerides, diacylglycerols, and phospholipid.
authors: Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi, USA.
authors: Rodney C
authors: RC
authors: Baker
authors: Division of Endocrinology, Department of Medicine, University of Mississippi Medical Center, Jackson, Mississippi, USA.
authors: Yana
authors: Y
authors: Nikitina
authors: Division of Endocrinology, Department of Medicine, University of Mississippi Medical Center, Jackson, Mississippi, USA. Electronic address: asubauste@umc.edu.
authors: Angela R
authors: AR
authors: Subauste
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods; D004075:Diglycerides / Q000032:analysis; D006801:Humans; D008055:Lipids / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D010743:Phospholipids / Q000032:analysis; D014280:Triglycerides / Q000032:analysis
chemical_list: D004075:Diglycerides; D008055:Lipids; D010743:Phospholipids; D014280:Triglycerides
keywords: Cyclic phosphatidic acid; Diacylglycerides; Lysophosphatidic acid; Phospholipids; Triglycerides