title: Role of unphosphorylated transcription factor STAT3 in late cerebral ischemia after subarachnoid hemorrhage.
abstract: Molecular mechanisms behind increased cerebral vasospasm and local inflammation in late cerebral ischemia after subarachnoid hemorrhage (SAH) are poorly elucidated. Using system biology tools and experimental SAH models, we have identified signal transducer and activator of transcription 3 (STAT3) transcription factor as a possible major regulatory molecule. On the basis of the presence of transcription factor binding sequence in the promoters of differentially regulated genes (significant enrichment PE: 6 × 10(5)) and the consistent expression of STAT3 (mRNA, P=0.0159 and Protein, P=0.0467), we hypothesize that unphosphorylated STAT3 may directly DNA bind and probably affect the genes that are involved in inflammation and late cerebral ischemia to influence the pathologic progression of SAH.
authors: Department of Clinical Experimental Research, Glostrup Research Institute, University of Copenhagen, Glostrup, Denmark.
authors: Ajoy K
authors: AK
authors: Samraj
authors: Department of Clinical Experimental Research, Glostrup Research Institute, University of Copenhagen, Glostrup, Denmark.
authors: Anne H
authors: AH
authors: Müller
authors: Department of Clinical Experimental Research, Glostrup Research Institute, University of Copenhagen, Glostrup, Denmark.
authors: Anne-Sofie
authors: AS
authors: Grell
authors: 1] Department of Clinical Experimental Research, Glostrup Research Institute, University of Copenhagen, Glostrup, Denmark [2] Division of Experimental Vascular Research, Department of Clinical Sciences, Lund University, Lund, Sweden.
authors: Lars
authors: L
authors: Edvinsson
mesh_terms: D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002545:Brain Ischemia / Q000209:etiology* / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D053614:Janus Kinase 2 / Q000235:genetics; D008297:Male; D010766:Phosphorylation; D011401:Promoter Regions, Genetic; D051381:Rats; D017207:Rats, Sprague-Dawley; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D013345:Subarachnoid Hemorrhage / Q000150:complications*; D015533:Transcriptional Activation
chemical_list: D050796:STAT3 Transcription Factor; D053614:Janus Kinase 2