title: Using multibiomarker approach as a tool to improve the management plan for a Private Reserve of Natural Heritage (RPPN).
abstract: This study aimed to monitor an aquatic ecosystem during two different periods (dry and rainy season) on a protected area located inside a sugarcane farm, using the fish Astyanax sp. as bioindicator. An integrated approach was adopted by combining the responses of well-known biomarkers: acetylcholinesterase, lipid peroxidation (LPO), catalase (CAT), glutathione S-transferase (GST), micronucleus test, and liver histopathology. The activity of enzymes CAT and GST was increased after the rainy season. This can be explained mainly by the intensification of rain density, which drags substances into the streams, especially pesticides applied on agriculture. LPO and micronucleus test also suggested some effects of contamination in the surrounding area during this season. The results have supported a discussion about the effectiveness of protected areas in agricultural regions, emphasizing the biomonitoring as a tool for improving management plans in protected areas.
authors: Ecology and Conservation Program of Post-Graduation, Federal University of Paraná, Curitiba, PR, Brazil.
authors: Manuela Dreyer
authors: MD
authors: da Silva
authors: Stéfani Cibele
authors: SC
authors: Rossi
authors: Nédia de Castilhos
authors: Nde C
authors: Ghisi
authors: Ciro Alberto
authors: CA
authors: de Oliveira Ribeiro
authors: Marta Margarete
authors: MM
authors: Cestari
authors: Helena Cristina
authors: HC
authors: Silva de Assis
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D003247:Conservation of Natural Resources / Q000379:methods*; D004784:Environmental Monitoring / Q000379:methods*; D005982:Glutathione Transferase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D014874:Water Pollutants, Chemical; D002374:Catalase; D005982:Glutathione Transferase; D000110:Acetylcholinesterase