title: Anti-proliferative action of silibinin on human colon adenomatous cancer HT-29 cells.
abstract: BACKGROUND
  Silibinin a flavonoid from milk thistle (Silybum marianum) exhibit a variety of pharmacological actions, including anti-proliferative and apoptotic activities against various types of cancers in intact animals and cancer cell lines. In the present study, the effect of silibinin on human colon cancer HT-29 cells was studied.
  
  
  METHODS
  Incubations of cells with different silibinin concentrations (0.783-1,600 ug/ml) for 24, 48 or 72 h showed a progressive decline in cell viability.
  
  
  RESULTS
  Loss of cell viability was time dependent and optimum inhibition of cell growth (78%) was observed at 72 h. Under inverted microscope, the dead cells were seen as cell aggregates. IC50 (silibinin concentration killing 50% cells) values were 180, 110 and 40 ug/ml at 24, 48 and 72 h respectively.
  
  
  CONCLUSIONS
  These findings re-enforce the anticancer potential of silibinin, as reported earlier for various other cancer cell lines (Ramasamy and Agarwal (2008), Cancer Letters, 269: 352-62).
authors: Department of Pharmacology Postgraduate Institute of Medical Education and Research. Chandigarh.. sanyalpu@gmail.com.
authors: Reyhan
authors: R
authors: Akhtar
authors: Faculty of Pharmacy. Jamia Hamdard. New Delhi.. sanyalpu@gmail.com.
authors: Mohd
authors: M
authors: Ali
authors: Department of Experimental Medicine and Biotechnology. Postgraduate Institute of Medical Education and Research. Chandigarh.. sanyalpu@gmail.com.
authors: Safrunnisa
authors: S
authors: Mahmood
authors: Department of Biophysics. Panjab University. Chandigarh.. sanyalpu@gmail.com.
authors: Sankar Nath
authors: SN
authors: Sanyal
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D019073:HT29 Cells; D006801:Humans; D000077385:Silybin; D012838:Silymarin / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D012838:Silymarin; D000077385:Silybin