title: Synergistic combination of marine oligosaccharides and azithromycin against Pseudomonas aeruginosa.
abstract: In this paper we describe how utilization of low molecular weight alginate-derived oligosaccharide (ADO) and chito-oligosaccharide (COS) in conjunction with antibiotics, could more effectively inhibit the growth of wild-type and resistant Pseudomonas aeruginosa. Inhibition is effected by modulating the bacteria's quorum sensing (QS) system, thus regulating biofilm formation and reducing resistance to antibiotic treatment. This can be demonstrated by using conventional MIC screening. COS showed synergistic effects with azithromycin, whereas ADO indicated additive effects against wild-type P. aeruginosa. Using electrospray-ionization mass spectroscopy (ESI-MS), matrix-assisted laser desorption/ionization-time of flightmass spectroscopy (MALDI-TOF-MS) and nuclear magnetic resonance (NMR), the chemical structure of ADO and of COS was characterized. The wild-type and resistant strains were identified by 16S rRNA sequence analysis. This report demonstrates the feasibility of attenuating the tolerance of P. aeruginosa to azithromycin by using specific marine oligosaccharides.
authors: College of Food Science and Engineering, Ocean University of China, Qingdao 266003, People's Republic of China; Department of Biology, Jackson State University, Jackson, MS 39217, United States.
authors: Xiaojia
authors: X
authors: He
authors: Department of Biology, Jackson State University, Jackson, MS 39217, United States.
authors: Huey-min
authors: HM
authors: Hwang
authors: Department of Biology, Jackson State University, Jackson, MS 39217, United States.
authors: Winfred G
authors: WG
authors: Aker
authors: College of Food Science and Engineering, Ocean University of China, Qingdao 266003, People's Republic of China.
authors: Peng
authors: P
authors: Wang
authors: Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS 39217, United States.
authors: Yunfeng
authors: Y
authors: Lin
authors: College of Food Science and Engineering, Ocean University of China, Qingdao 266003, People's Republic of China. Electronic address: jiangxl@ouc.edu.cn.
authors: Xiaolu
authors: X
authors: Jiang
authors: College of Food Science and Technology, Huazhong Agriculture University, Wuhan 430070, People's Republic of China.
authors: Xiaoyu
authors: X
authors: He
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017963:Azithromycin / Q000494:pharmacology*; D018441:Biofilms / Q000254:growth & development; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004357:Drug Synergism; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D053038:Quorum Sensing / Q000187:drug effects; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013312:Stress, Physiological
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D009844:Oligosaccharides; D012336:RNA, Ribosomal, 16S; D017963:Azithromycin
keywords: Alginate-derived oligosaccharide; Antibiotics; Chito-oligosaccharides; Pseudomonas aeruginosa; Quorum sensing