title: Rat cortex and hippocampus-derived soluble factors for the induction of adipose-derived mesenchymal stem cells into neuron-like cells.
abstract: To simulate brain microenvironment, adipose-derived mesenchymal stem cells (AMSC) were induced to differentiate to neuronal-like cells in rat cortex and hippocampus medium (Cox + Hip). First, isolated AMSC were characterized by flow cytometer and the capacity of adipogenesis and osteogenesis. After induction in rat cortex and hippocampus conditioned medium, the cell morphological change was examined and neural marker proteins (β-Ш-Tubulin, NSE, Nissl body) expression was detected by immunofluorescence staining. A variety of synaptic marker proteins, including GAP43, SHANK2, SHANK3 and Bassoon body, were detected. ELISA was used to measure brain derived neurotrophic factor (BDNF) and nerve growth factor (NGF) secretion at different time-points. AMSCs positively expressed CD13, CD44 and CD90 and could differentiate into osteoblasts or adipocytes. After induction in Cox + Hip medium for 14 days, cells had a typical neuronal perikaryal appearance, which was suggestive of neuronal differentiation. After 14 days of Cox + Hip treatment, the percentage of cells expressing β-Ⅲ-Tubulin, NSE and Nissl was 53.9 ± 0.8%, 51.3 ± 1.7% and 16.4 ± 2.1%, respectively. Expression of GAP43, SHANK2, SHANK3 and Bassoon body was detected, indicating synapse formation after treatment in Cox + Hip medium. Differentiated AMSCs secreted neurotrophic factors NGF and BDNF. Thus rat cortex and hippocampus-derived soluble factors can induce AMSCs to a neuronal-like phenotype, suggesting that AMSCs have a dual role in supplementing newborn neurons and secreting neurotrophic factors, and therefore could be help as a potential treatment for nervous system diseases.
authors: Regenerative Medicine Centre, First Affiliated Hospital of Dalian Medical University, Dalian, 116011, P.R. China; Institute of Integrative Medicine, Dalian Medical University, Dalian, 116011, P.R. China.
authors: Chao
authors: C
authors: Han
authors: Lin
authors: L
authors: Song
authors: Yang
authors: Y
authors: Liu
authors: Wei
authors: W
authors: Zou
authors: Chen
authors: C
authors: Jiang
authors: Jing
authors: J
authors: Liu
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000328:Adult; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D018826:CD13 Antigens / Q000096:biosynthesis; D002525:Cerebellar Cortex / Q000166:cytology / Q000378:metabolism; D019922:GAP-43 Protein / Q000096:biosynthesis; D006624:Hippocampus / Q000166:cytology*; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D008875:Middle Aged; D020932:Nerve Growth Factor / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D055495:Neurogenesis / Q000502:physiology*; D009562:Nissl Bodies / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology; D051381:Rats; D018800:Thy-1 Antigens / Q000096:biosynthesis; D014404:Tubulin / Q000096:biosynthesis; D055815:Young Adult
chemical_list: D019208:Brain-Derived Neurotrophic Factor; C113688:Bsn protein, rat; D019922:GAP-43 Protein; D018960:Hyaluronan Receptors; D009419:Nerve Tissue Proteins; C485686:Shank2 protein, rat; C120532:Shank3 protein, rat; D018800:Thy-1 Antigens; C488416:Tubb3 protein, rat; D014404:Tubulin; D020932:Nerve Growth Factor; D018826:CD13 Antigens
keywords: adipose-derived mesenchymal stem cells (AMSCs); cortex and hippocampus- conditioned medium; neuronal differentiation