title: Transcriptome profile of the murine macrophage cell response to Candida parapsilosis.
abstract: Candida parapsilosis is a human fungal pathogen with increasing global significance. Understanding how macrophages respond to C. parapsilosis at the molecular level will facilitate the development of novel therapeutic paradigms. The complex response of murine macrophages to infection with C. parapsilosis was investigated at the level of gene expression using an Agilent mouse microarray. We identified 155 and 511 differentially regulated genes at 3 and 8h post-infection, respectively. Most of the upregulated genes encoded molecules involved in immune response and inflammation, transcription, signaling, apoptosis, cell cycle, electron transport and cell adhesion. Typical of the classically activated macrophages, there was significant upregulation of genes coordinating the production of inflammatory cytokines such as TNF, IL-1 and IL-15. Further, we used both primary murine macrophages and macrophages differentiated from human peripheral mononuclear cells to confirm the upregulation of the TNF-receptor family member TNFRSF9 that is associated with Th1 T-helper cell responses. Additionally, the microarray data indicate significant differences between the response to C. parapsilosis infection and that of C. albicans.
authors: Department of Microbiology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary.
authors: Tibor
authors: T
authors: Németh
authors: Department of Microbiology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary.
authors: Adél
authors: A
authors: Tóth
authors: Department of Microbiology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary.
authors: Zsuzsanna
authors: Z
authors: Hamari
authors: Department of Genetics, Cell- and Immunobiology, Semmelweis University, Nagyvárad tér 4, 1089 Budapest, Hungary.
authors: András
authors: A
authors: Falus
authors: Department of Genetics, Cell- and Immunobiology, Semmelweis University, Nagyvárad tér 4, 1089 Budapest, Hungary.
authors: Katalin
authors: K
authors: Éder
authors: Department of Microbiology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary.
authors: Csaba
authors: C
authors: Vágvölgyi
authors: Department of Microbiology and Immunology, Albert Einstein College of Medicine, 1300 Morris Park Ave, New York, NY 10461, USA.
authors: Allan J
authors: AJ
authors: Guimaraes
authors: Department of Microbiology and Immunology, Albert Einstein College of Medicine, 1300 Morris Park Ave, New York, NY 10461, USA.
authors: Joshua D
authors: JD
authors: Nosanchuk
authors: Department of Microbiology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary. Electronic address: gacsera@gmail.com.
authors: Attila
authors: A
authors: Gácser
mesh_terms: D000818:Animals; D002175:Candida / Q000502:physiology*; D002478:Cells, Cultured; D006801:Humans; D008264:Macrophages / Q000378:metabolism* / Q000382:microbiology* / Q000473:pathology; D051379:Mice; D010587:Phagocytosis; D059467:Transcriptome; D053261:Tumor Necrosis Factor Receptor Superfamily, Member 9 / Q000235:genetics / Q000378:metabolism
chemical_list: C505714:TNFRSF9 protein, human; D053261:Tumor Necrosis Factor Receptor Superfamily, Member 9
keywords: Candida parapsilosis; Host response; Infection; Macrophages