title: Capilliposide Isolated from Lysimachia capillipes Hemsl. Induces ROS Generation, Cell Cycle Arrest, and Apoptosis in Human Nonsmall Cell Lung Cancer Cell Lines.
abstract: Several data has reported that capilliposide, extracted from a traditional Chinese medicine, Lysimachia capillipes Hemsl. (LC) could exhibit inhibitory effect on cell proliferation in various cancers. The current study investigated the antitumor efficacy of Capilliposide and elucidated its potential molecular mechanism involved in vivo and vitro. Our results indicated that LC capilliposide inhibited proliferation of lung cancer cells in a dose-dependent manner. LC capilliposide induced cell cycle arrest at the S stage and enhanced apoptosis in NSCLC cells. Treatment with LC capilliposide increased the intracellular level of ROS, which activated the mitochondrial apoptotic pathway. Blockage of ROS by NAC highly reversed the effect of LC capilliposide on apoptosis. Xenograft tumor growth was significantly lower in the LC-treated group compared with the untreated control group (P < 0.05). The results also show that LC treatment does not produce any overt signs of acute toxicity in vivo. These findings demonstrate that LC capilliposide could exert an anti-tumor effect on NSCLC through mitochondrial-mediated apoptotic pathway and the activation of ROS is involved.
authors: Department of Radiation Oncology and Chemotherapy, The First Affiliated Hospital of Wenzhou Medical University, No. 2 Fuxue Road, Wenzhou 325000, China.
authors: Zheng-Hua
authors: ZH
authors: Fei
authors: Zhejiang Chinese Medical University, No. 548 Binwen Road, Hangzhou 310053, China.
authors: Kan
authors: K
authors: Wu
authors: Wenzhou Medical University, No. 58 Chashan Road, Wenzhou 325035, China.
authors: Yun-Liang
authors: YL
authors: Chen
authors: Zhejiang Chinese Medical University, No. 548 Binwen Road, Hangzhou 310053, China.
authors: Bing
authors: B
authors: Wang
authors: Department of Oncology, The Hangzhou First People's Hospital, No. 261 Huansha Road, Hangzhou 310006, China.
authors: Shi-Rong
authors: SR
authors: Zhang
authors: Department of Oncology, The Hangzhou First People's Hospital, No. 261 Huansha Road, Hangzhou 310006, China.
authors: Sheng-Lin
authors: SL
authors: Ma