title: Enhanced production of 3-hydroxypropionic acid from glycerol by modulation of glycerol metabolism in recombinant Escherichia coli.
abstract: 3-Hydroxypropionic acid (3-HP) is a valuable biochemical with high potential for bioplastic manufacturing. The endogenous glycerol metabolism and by-product formation pathway in Escherichia coli were modulated to enhance 3-HP production from glycerol. Double deletion of glpK and yqhD directed the glycerol flux to 3-HP biosynthesis and reduced the formation of 1,3-propanediol. Since 3-hydroxypropionaldehyde (3-HPA), a precursor of 3-HP, is toxic to cell growth, the gene encoding Pseudomonas aeruginosa semialdehyde dehydrogenase (PSALDH) highly active on 3-HPA was expressed in E. coli. Finally, fed-batch culture of recombinant E. coli BL21star(DE3) without glpK and yqhD, and expressing Lactobacillus brevis DhaB-DhaR, and P. aeruginosa PSALDH resulted in 57.3g/L 3-HP concentration, 1.59g/L-h productivity and 0.88g/g yield. In conclusion, modulation of the glycerol metabolism in combination with enhanced activity of 3-HPA dehydrogenation improved the production of 3-HP from glycerol.
authors: Interdisciplinary Program of Bioengineering, Seoul National University, Seoul 151-921, South Korea.
authors: Kwangwook
authors: K
authors: Kim
authors: Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, Seoul 151-921, South Korea.
authors: Sun-Ki
authors: SK
authors: Kim
authors: Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul 136-702, South Korea. Electronic address: ycpark@kookmin.ac.kr.
authors: Yong-Cheol
authors: YC
authors: Park
authors: Interdisciplinary Program of Bioengineering, Seoul National University, Seoul 151-921, South Korea; Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, Seoul 151-921, South Korea. Electronic address: jhseo94@snu.ac.kr.
authors: Jin-Ho
authors: JH
authors: Seo
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000378:metabolism; D061249:Batch Cell Culture Techniques; D001681:Biological Assay; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation; D017353:Gene Deletion; D005798:Genes, Bacterial; D005985:Glyceraldehyde / Q000031:analogs & derivatives / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D019344:Lactic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis; D053858:Metabolic Networks and Pathways; D010084:Oxidation-Reduction; D011407:Propane / Q000378:metabolism; D011995:Recombination, Genetic / Q000235:genetics*; D013379:Substrate Specificity
chemical_list: C047158:3-hydroxypropionaldehyde; D019344:Lactic Acid; D005985:Glyceraldehyde; C031601:hydracrylic acid; D000444:Aldehyde Dehydrogenase; D005990:Glycerol; D011407:Propane
keywords: 3-Hydroxypropionic acid; Escherichia coli; Fed-batch fermentation; Glycerol; Glycerol metabolism