title: Effect of RSCs combined with COP-1 on optic nerve damage in glaucoma rat model.
abstract: OBJECTIVE
  To explore effect of retinal stem cells (RSCs) combined with copolymer-1 (COP-1) immunotherapy on optic nerve damage in glaucoma rat model.
  
  
  METHODS
  A total of 40 SD rats were selected for glaucoma model and were randomly divided into 4 groups to observe protective effects of RSCs transplantation combined with COP-1.
  
  
  RESULTS
  Brain-derived neurotrophic factor (BDNF) and insulin like growth factor-1 (IGF-1) were either positive in retina of RSCs transplanted or COP-1 immunological treated rat. Positive rate of BDNF and IGF-1 and expression of mRNA and protein were significantly higher in RSCs transplantation combined with COP-1 immunotherapy treated rats compared with the other 3 groups, in which amount of apoptotic RGCs was lowest.
  
  
  CONCLUSIONS
  RSCs transplantation combined with COP-1 immunotherapy can promote the secretion of BDNF and IGF-1. They protect RGCs in glaucoma rats in coordination, significantly reduce the number of apoptosis RGCs so as to alleviate the optic nerve damage. It ponits a new research direction for treatment of glaucoma.
authors: Department of Ophthalmology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
authors: Wei-Cai
authors: WC
authors: Fu
authors: Department of Ophthalmology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
authors: Yan
authors: Y
authors: Jiang
authors: Department of Ophthalmology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China. Electronic address: 28906390@qq.com.
authors: Lin
authors: L
authors: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D002478:Cells, Cultured; D016009:Chi-Square Distribution; D004195:Disease Models, Animal; D005260:Female; D000068717:Glatiramer Acetate; D005901:Glaucoma / Q000276:immunology / Q000601:surgery / Q000628:therapy*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D008297:Male; D008856:Microscopy, Fluorescence; D020221:Optic Nerve Injuries / Q000276:immunology / Q000601:surgery / Q000628:therapy*; D010455:Peptides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000737:chemistry / Q000166:cytology*; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D010455:Peptides; D012333:RNA, Messenger; D000068717:Glatiramer Acetate; D007334:Insulin-Like Growth Factor I
keywords: Glaucoma model; Immunotherapy; Polymer −1; Rat; Retinal stem cells