title: Cytotoxicity of diplodiatoxin, dipmatol and diplonine, metabolites synthesized by Stenocarpella maydis.
abstract: The cytotoxicity of three Stenocarpella maydis metabolites (diplodiatoxin, dipmatol and diplonine) was investigated on Neuro-2a, CHO-K1 and MDBK cell lines. Diplodiatoxin was the most cytotoxic followed by dipmatol. Conversely, diplonine was not cytotoxic. Diplodiatoxin and dipmatol affected mitochondrial succinate dehydrogenase (MTT assay) and the overall viability of cells as assessed in real-time (xCELLigence assay). The results obtained so far indicate that diplodiatoxin and dipmatol exert their toxicity possibly via the necrotic cell death pathway.
authors: Toxicology and Ethnoveterinary Medicine, Food, Feed and Veterinary Public Health (FFVPH) Programme, Agricultural Research Council-Onderstepoort Veterinary Institute (ARC-OVI), P/Bag x05, Onderstepoort, 0110, South Africa; Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, P/Bag x04, Onderstepoort, 0110, South Africa. Electronic address: MasangoM@arc.agric.za.
authors: Mxolisi G
authors: MG
authors: Masango
authors: Toxicology and Ethnoveterinary Medicine, Food, Feed and Veterinary Public Health (FFVPH) Programme, Agricultural Research Council-Onderstepoort Veterinary Institute (ARC-OVI), P/Bag x05, Onderstepoort, 0110, South Africa.
authors: Gezina C H
authors: GC
authors: Ferreira
authors: Molecular Epidemiology and Diagnostics (MED) Programme, Agricultural Research Council-Onderstepoort Veterinary Institute (ARC-OVI), P/Bag x05, Onderstepoort, 0110, South Africa.
authors: Charlotte E
authors: CE
authors: Ellis
authors: Toxicology and Ethnoveterinary Medicine, Food, Feed and Veterinary Public Health (FFVPH) Programme, Agricultural Research Council-Onderstepoort Veterinary Institute (ARC-OVI), P/Bag x05, Onderstepoort, 0110, South Africa; Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, P/Bag x04, Onderstepoort, 0110, South Africa.
authors: Esameldin E
authors: EE
authors: Elgorashi
authors: Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, P/Bag x04, Onderstepoort, 0110, South Africa.
authors: Christo J
authors: CJ
authors: Botha
mesh_terms: D000818:Animals; D001203:Ascomycota / Q000378:metabolism*; D016466:CHO Cells; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002867:Chromones / Q000378:metabolism / Q000633:toxicity*; D006224:Cricetinae; D003412:Cricetulus; D003521:Cyclopropanes / Q000378:metabolism / Q000633:toxicity*; D004285:Dogs; D006168:Guinea Pigs; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D009336:Necrosis / Q000139:chemically induced / Q000473:pathology; D010935:Plant Diseases / Q000382:microbiology; D013385:Succinate Dehydrogenase / Q000378:metabolism; D003313:Zea mays
chemical_list: D002867:Chromones; D003521:Cyclopropanes; C456848:diplodiatoxin; C568540:diplonine; D013385:Succinate Dehydrogenase
keywords: Cytotoxicity; Diplodiatoxin; Diplonine; Dipmatol; Stenocarpella maydis; xCELLigence