title: Detection of Anaplasma platys in dogs using real-time loop-mediated isothermal amplification.
abstract: Anaplasma platys is a parasite of canine platelets that causes infectious cyclic thrombocytopenia. In this study, a novel real-time loop-mediated isothermal amplification (RT-LAMP) method was developed to detect A. platys. RT-LAMP primer sets were designed using a citrate synthase gene sequence and the assay was performed at 63 °C for 30 min. No cross-reactivity was observed with other Anaplasma or Ehrlichia spp. and the method exhibited a similar level of sensitivity in detecting the organism in 58 canine blood samples to that of a nested PCR. This RT-LAMP is a rapid and potentially cost-effective method of diagnosing A. platys infection in dogs.
authors: College of Veterinary Medicine, South China Agricultural University, 483 Wushan Street, Guangzhou 510642, China.
authors: Hua-tao
authors: HT
authors: Li
authors: College of Veterinary Medicine, South China Agricultural University, 483 Wushan Street, Guangzhou 510642, China.
authors: Ling-suang
authors: LS
authors: Sun
authors: College of Veterinary Medicine, South China Agricultural University, 483 Wushan Street, Guangzhou 510642, China.
authors: Zhong-ming
authors: ZM
authors: Chen
authors: College of Veterinary Medicine, South China Agricultural University, 483 Wushan Street, Guangzhou 510642, China.
authors: Jing-si
authors: JS
authors: Hu
authors: College of Veterinary Medicine, South China Agricultural University, 483 Wushan Street, Guangzhou 510642, China.
authors: Cun-dong
authors: CD
authors: Ye
authors: College of Veterinary Medicine, South China Agricultural University, 483 Wushan Street, Guangzhou 510642, China.
authors: Kun
authors: K
authors: Jia
authors: College of Veterinary Medicine, South China Agricultural University, 483 Wushan Street, Guangzhou 510642, China.
authors: Heng
authors: H
authors: Wang
authors: College of Veterinary Medicine, South China Agricultural University, 483 Wushan Street, Guangzhou 510642, China.
authors: Li-guo
authors: LG
authors: Yuan
authors: College of Veterinary Medicine, South China Agricultural University, 483 Wushan Street, Guangzhou 510642, China.
authors: Gui-hong
authors: GH
authors: Zhang
authors: College of Veterinary Medicine, South China Agricultural University, 483 Wushan Street, Guangzhou 510642, China. Electronic address: shoujunli@scau.edu.cn.
authors: Shoujun
authors: S
authors: Li
mesh_terms: D000709:Anaplasma / Q000235:genetics / Q000302:isolation & purification*; D000712:Anaplasmosis / Q000175:diagnosis* / Q000469:parasitology; D000818:Animals; D002681:China; D004283:Dog Diseases / Q000175:diagnosis* / Q000469:parasitology; D004285:Dogs; D004536:Ehrlichia / Q000235:genetics / Q000302:isolation & purification; D021141:Nucleic Acid Amplification Techniques / Q000191:economics / Q000662:veterinary*; D012680:Sensitivity and Specificity
keywords: Anaplasma platys; Canine; Real-time loop-mediated isothermal amplification; gltA gene