title: CD8(+) T cells mediate robust stage-specific immunity to P. berghei under chemoprophylaxis and this protective environment is not downregulated by the presence of blood-stage infection.
abstract: Sterile protection against malaria infection can be achieved by the inoculation of intact sporozoites while treating concomitantly with the 4-aminoquinoline chloroquine. We present an analysis of protective immunity elicited by successive immunization with Plasmodium berghei sporozoites under chemoprophylaxis. Immunization resulted in a protective, stage-specific immune response. Protection appeared to be mediated by CD8(+) T cells and was abrogated upon their specific depletion. Adoptive transfer of splenocytes rendered recipient animals resistant to sporozoite infection, but not to blood-stage challenge. Immunization with sporozoites under chemoprophylaxis results in robust immunity, and the presence of blood-stage infection at sporozoite immunization had no downregulating effect on the protective immune response.
authors: Department of Infectious Diseases, Parasitology Unit, University Hospital Heidelberg, Heidelberg, Germany.
authors: Matthew D
authors: MD
authors: Lewis
authors: Department of Infectious Diseases, Parasitology Unit, University Hospital Heidelberg, Heidelberg, Germany ; Centre for Childhood and Adolescence Medicine, University Hospital Heidelberg, Heidelberg, Germany.
authors: Johannes
authors: J
authors: Pfeil
authors: Department of Infectious Diseases, Parasitology Unit, University Hospital Heidelberg, Heidelberg, Germany.
authors: Kirsten
authors: K
authors: Heiss
authors: Department of Infectious Diseases, Parasitology Unit, University Hospital Heidelberg, Heidelberg, Germany.
authors: Ann-Kristin
authors: AK
authors: Mueller
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000494:pharmacology / Q000627:therapeutic use*; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D018890:Chemoprevention; D002738:Chloroquine / Q000494:pharmacology / Q000627:therapeutic use*; D003131:Combined Modality Therapy; D007114:Immunization; D007156:Immunologic Memory / Q000187:drug effects / Q000276:immunology; D008288:Malaria / Q000276:immunology / Q000517:prevention & control*; D017780:Malaria Vaccines / Q000627:therapeutic use*; D051379:Mice; D010962:Plasmodium berghei; D034101:Sporozoites / Q000276:immunology
chemical_list: D000962:Antimalarials; D017780:Malaria Vaccines; D002738:Chloroquine