title: Knocking down the expression of adenylate cyclase-associated protein 1 inhibits the proliferation and migration of breast cancer cells.
abstract: Adenylate cyclase-associated protein 1 (CAP1) is a conserved protein that was found to be up-regulated in breast cancer and related to the migration of breast cancer. We verified its roles in breast cancer specimens and cell lines. In our results, 71 of 100 specimens of breast cancer showed high levels of CAP1 by immunohistochemistry. Associated with statistical analysis, we saw that CAP1 was related to the grade of breast cancer. In MDA-MB-231, the expression of CAP1 was the highest and by knocking down the expression of CAP1 in MDA-MB-231, its ability for proliferating and migrating apparently decreased and induced changes in morphology, which were related to the arrangement of F-actin. Therefore, CAP1 might be a potential molecular targeted therapy for surgery and immune treatment.
authors: Department of General Surgery, The Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong 226001, Jiangsu, China.
authors: Xia-Fei
authors: XF
authors: Yu
authors: Department of General Surgery, The Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong 226001, Jiangsu, China.
authors: Qi-Chao
authors: QC
authors: Ni
authors: Department of General Surgery, The Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong 226001, Jiangsu, China.
authors: Jin-Peng
authors: JP
authors: Chen
authors: Department of General Surgery, The Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong 226001, Jiangsu, China.
authors: Jun-Fei
authors: JF
authors: Xu
authors: Department of Pathogen Biology, Medical College, Nantong University, Nantong 226001, Jiangsu, China.
authors: Ying
authors: Y
authors: Jiang
authors: Department of Pathology, Nantong University Cancer Hospital, Nantong 226363, Jiangsu, China.
authors: Shu-Yun
authors: SY
authors: Yang
authors: Department of General Surgery, The Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong 226001, Jiangsu, China.
authors: Jing
authors: J
authors: Ma
authors: Department of Pathogen Biology, Medical College, Nantong University, Nantong 226001, Jiangsu, China.
authors: Xiao-Ling
authors: XL
authors: Gu
authors: Department of General Surgery, The Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong 226001, Jiangsu, China. Electronic address: 13706292250@163.com.
authors: Hua
authors: H
authors: Wang
authors: Department of Pathogen Biology, Medical College, Nantong University, Nantong 226001, Jiangsu, China. Electronic address: wyy0418@ntu.edu.cn.
authors: Ying-Ying
authors: YY
authors: Wang
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000096:biosynthesis / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation; D003598:Cytoskeletal Proteins / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D058990:Molecular Targeted Therapy
chemical_list: C489549:CAP1 protein, human; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins
keywords: Adenylate cyclase-associated protein 1; Breast cancer; F-actin; Migration; Proliferation