title: [The inhibitory effect of matrine on the growth of human colorectal cancer HT29 cells: an experimental observation].
abstract: OBJECTIVE
  To study the inhibitory and inductive effect of matrine (MA) on human colorectal cancer HT29 cells.
  
  
  METHODS
  MTT assay was used to determine the cell growth inhibitory rate in vitro . Changes of cell cycle and the apoptosis of HT29 cells before and after MA treatment were observed using flow cytometry and electron microscope.
  
  
  RESULTS
  MTT showed that 2-32 mg/mL MA inhibited the proliferation of HT29 cells (P < 0.05) in a dose- and time-dependent manner. Cells treated by 4, 8, and 16 mg/mL MA at G0/G1 phase were obviously higher than those in the negative control group (P < 0.05), indicating that the cell cycle was arrested at G0/G1 phase. Morphological apoptosis of HT29 cells could be seen under transmission electron microscope.
  
  
  CONCLUSIONS
  MA inhibited the proliferation of HT29 cells, and its mechanism might be associated with stagnation at G0/G1 phase and inducing apoptosis of HT29 cells.
authors: Department of MRI Room and Gastroenterology, Huangshi Central Hospital, Hubei College of Technology, Hubei 435000, China.
authors: Cheng
authors: C
authors: Chang
authors: Department of MRI Room and Gastroenterology, Huangshi Central Hospital, Hubei College of Technology, Hubei 435000, China.
authors: De-Li
authors: DL
authors: Rao
authors: Department of MRI Room and Gastroenterology, Huangshi Central Hospital, Hubei College of Technology, Hubei 435000, China.
authors: Xiao-Ming
authors: XM
authors: Qiu
authors: Department of MRI Room and Gastroenterology, Huangshi Central Hospital, Hubei College of Technology, Hubei 435000, China.
authors: Hong
authors: H
authors: Wang
authors: Hubei Key Laboratory of Kidney Disease Pathogenesis and Intervention, Hubei College of Technology, Hubei 435003, China.
authors: Li
authors: L
authors: Xiong
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D019073:HT29 Cells; D006801:Humans; D011807:Quinolizines / Q000494:pharmacology*; D000093842:Matrines
chemical_list: D000470:Alkaloids; D011807:Quinolizines; D000093842:Matrines