title: Repair of the 3' proximal and internal deletions of a satellite RNA associated with Cucumber mosaic virus is directed toward restoring structural integrity.
abstract: The phenomenon of rapid turnover of 3' proximal nucleotides (nt) lost by the action of nuclease in RNA viruses is integral to replication. Here, a set of six deletions encompassing the 3' 23 nt region of a satellite RNA (satRNA) of Cucumber mosaic virus (CMV) strain Q (Q-sat), were engineered. Repair of the 3' end was not observed in the absence of CMV. However, co-expression with CMV in planta revealed that Q-sat mutants lacking the 3' 18 nt but not the 3' 23 nt are repaired and the progeny accumulation was inversely proportional to the extent of the deletion. Progeny of the 3'Δ3 mutant were repaired to wild type (wt) while those from the remaining four mutants were heterogeneous, exhibiting a wt secondary structure. Analysis of additional 3' internal deletions mutants revealed that progeny with a repaired sequence reminiscent of wt secondary structure were competent for replication and systemic spread.
authors: Department of Plant Pathology & Microbiology, University of California, Riverside, CA 92521-0122, United States.
authors: Sun-Jung
authors: SJ
authors: Kwon
authors: Department of Plant Pathology & Microbiology, University of California, Riverside, CA 92521-0122, United States.
authors: Sonali
authors: S
authors: Chaturvedi
authors: Department of Plant Pathology & Microbiology, University of California, Riverside, CA 92521-0122, United States. Electronic address: arao@ucr.edu.
authors: A L N
authors: AL
authors: Rao
mesh_terms: D001483:Base Sequence; D019401:Cucumber Mosaic Virus Satellite / Q000235:genetics* / Q000378:metabolism; D017799:Cucumovirus / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004260:DNA Repair; D006378:Helper Viruses / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010935:Plant Diseases / Q000821:virology; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017384:Sequence Deletion; D014026:Nicotiana / Q000821:virology
chemical_list: D019401:Cucumber Mosaic Virus Satellite; D012367:RNA, Viral
keywords: 3' repair; Cucumovirus; RNA virus; Satellite RNA