title: Folding of synthetic homogeneous glycoproteins in the presence of a glycoprotein folding sensor enzyme.
abstract: UDP-glucose:glycoprotein glucosyltransferase (UGGT) plays a key role in recognizing folded and misfolded glycoproteins in the glycoprotein quality control system of the endoplasmic reticulum. UGGT detects misfolded glycoproteins and re-glucosylates them as a tag for misfolded glycoproteins. A flexible model to reproduce in vitro folding of a glycoprotein in the presence of UGGT in a mixture containing correctly folded, folding intermediates, and misfolded glycoproteins is described. The data demonstrates that UGGT can re-glucosylate all intermediates in the in vitro folding experiments, thus indicating that UGGT inspects not only final folded products, but also the glycoprotein folding intermediates.
authors: Department of Chemistry, Graduate School of Science, Institution Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka, 560-0043 (Japan); Institution Japan Science and Technology Agency (JST), ERATO, Ito Glycotrilogy Project, 2-1 Hirosawa, Wako, Saitama, 351-0198 (Japan).
authors: Simone
authors: S
authors: Dedola
authors: Masayuki
authors: M
authors: Izumi
authors: Yutaka
authors: Y
authors: Makimura
authors: Akira
authors: A
authors: Seko
authors: Akiko
authors: A
authors: Kanamori
authors: Masafumi
authors: M
authors: Sakono
authors: Yukishige
authors: Y
authors: Ito
authors: Yasuhiro
authors: Y
authors: Kajihara
mesh_terms: D004721:Endoplasmic Reticulum / Q000378:metabolism; D005964:Glucosyltransferases / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism; D017510:Protein Folding; D021241:Spectrometry, Mass, Electrospray Ionization; D013379:Substrate Specificity
chemical_list: D010940:Plant Proteins; C024934:crambin protein, Crambe abyssinica; D005964:Glucosyltransferases; C072423:mannosylglycoprotein 1,3-glucosyltransferase
keywords: biological activity; glycoproteins; mass spectrometry; molecular recognition; protein folding