title: Validation of a portable nitric oxide analyzer for screening in primary ciliary dyskinesias.
abstract: BACKGROUND
  Nasal nitric oxide (nNO) levels are very low in primary ciliary dyskinesia (PCD) and it is used as a screening test.
  
  
  METHODS
  We assessed the reliability and usability of a hand-held analyser in comparison to a stationary nitric oxide (NO) analyser in 50 participants (15 healthy, 13 PCD, 22 other respiratory diseases; age 6-79 years). Nasal NO was measured using a stationary NO analyser during a breath-holding maneuver, and using a hand-held analyser during tidal breathing, sampling at 2 ml/sec or 5 ml/sec. The three methods were compared for their specificity and sensitivity as a screen for PCD, their success rate in different age groups, within subject repeatability and acceptability. Correlation between methods was assessed.
  
  
  RESULTS
  Valid nNO measurements were obtained in 94% of participants using the stationary analyser, 96% using the hand-held analyser at 5 ml/sec and 76% at 2 ml/sec. The hand-held device at 5 ml/sec had excellent sensitivity and specificity as a screening test for PCD during tidal breathing (cut-off of 30 nL/min,100% sensitivity, >95% specificity). The cut-off using the stationary analyser during breath-hold was 38 nL/min (100% sensitivity, 95% specificity). The stationary and hand-held analyser (5 ml/sec) showed reasonable within-subject repeatability(% coefficient of variation = 15).
  
  
  CONCLUSIONS
  The hand-held NO analyser provides a promising screening tool for PCD.
authors: Amanda
authors: A
authors: Harris
authors: Esther
authors: E
authors: Bhullar
authors: Kerry
authors: K
authors: Gove
authors: Rhiannon
authors: R
authors: Joslin
authors: Jennifer
authors: J
authors: Pelling
authors: Hazel J
authors: HJ
authors: Evans
authors: Woolf T
authors: WT
authors: Walker
authors: Primary Ciliary Dyskinesia Centre, University Hospital Southampton NHS Foundation Trust, Southampton, UK. jlucas1@soton.ac.uk.
authors: Jane S
authors: JS
authors: Lucas
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D003948:Diagnostic Techniques, Respiratory System / Q000295:instrumentation; D006801:Humans; D007619:Kartagener Syndrome / Q000175:diagnosis* / Q000378:metabolism; D008875:Middle Aged; D009569:Nitric Oxide / Q000032:analysis*; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D009569:Nitric Oxide