title: Scavenger receptor-mediated endocytosis facilitates RNA interference in the desert locust, Schistocerca gregaria.
abstract: RNA interference (RNAi) has become a widely used loss-of-function tool in eukaryotes; however, the delivery of double-stranded (ds)RNA) to the target cells remains a major challenge when exploiting the RNAi-technology. In insects, the efficiency of RNAi is highly species-dependent. Yet, the mechanism of cell entry in insects has only been characterized in a cell line of the fruit fly, Drosophila melanogaster, a species that is well known to be poorly amenable to environmental RNAi. In the present paper, we demonstrate that silencing vacuolar H-ATPase 16 (vha16) and clathrin heavy chain (clath), two components of the Clathrin-dependent endocytosis pathway, together with pharmacological inhibition of scavenger receptors with polyinosine and dextran sulphate, can significantly attenuate the highly robust RNAi response in the desert locust, Schistocerca gregaria.
authors: Molecular Developmental Physiology and Signal Transduction, Department of Animal Physiology and Neurobiology, KU Leuven, Leuven, Belgium.
authors: N
authors: N
authors: Wynant
authors: D
authors: D
authors: Santos
authors: P
authors: P
authors: Van Wielendaele
authors: J
authors: J
authors: Vanden Broeck
mesh_terms: D000818:Animals; D002966:Clathrin; D016264:Dextran Sulfate; D004705:Endocytosis; D006110:Grasshoppers / Q000235:genetics*; D011069:Poly I; D034622:RNA Interference / Q000187:drug effects*; D012330:RNA, Double-Stranded / Q000378:metabolism; D051116:Receptors, Scavenger
chemical_list: D002966:Clathrin; D012330:RNA, Double-Stranded; D051116:Receptors, Scavenger; D011069:Poly I; D016264:Dextran Sulfate
keywords: RNAi; double stranded RNA (dsRNA); environmental; insect; pattern recognition; sid1; systemic