title: Requirement of Runx3 in pulmonary vasculogenesis.
abstract: Runx3 is essential for normal vertebrate lung development and Runx3 knockout (KO) mice die within 24 h after birth because of various organ defects including defects in alveolar expansion. For proper early lung development, vasculogenesis and angiogenesis are necessary in humans. Previous studies have reported that various signaling molecules, such as CD31, VEGF and vWF, are closely related to lung vasculogenesis and angiogenesis. To confirm the relationship between Runx3-related lung defects and vasculogenesis, the localization of various blood vessel markers is examined in WT and Runx3 KO mouse lungs at PN1. Our results indicate that CD31, VEGF and vWF were dramatically up-regulated by a loss of Runx3 during lung development. Moreover, U0126, a MEK inhibitor, rescued the lung phenotype and vascularization by regulation of ERK signaling. Therefore, it was concluded that lung vasculogenesis and angiogenesis were induced in the Runx3 KO mouse, which shows lung defects, by increased CD31, VEGF and vWF.
authors: Division in Anatomy and Developmental Biology, Department of Oral Biology, BK21 PLUS project, Oral Science Research Institute, College of Dentistry, Yonsei Center of Biotechnology, Yonsei University, Seoul, Korea.
authors: Jong-Min
authors: JM
authors: Lee
authors: Hyuk-Jae
authors: HJ
authors: Kwon
authors: Wing-Fu
authors: WF
authors: Lai
authors: Han-Sung
authors: HS
authors: Jung
mesh_terms: D000818:Animals; D002070:Butadienes / Q000494:pharmacology; D050677:Core Binding Factor Alpha 3 Subunit / Q000235:genetics*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000096:biosynthesis; D006965:Hyperplasia / Q000235:genetics; D008168:Lung / Q000098:blood supply* / Q000196:embryology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D020929:Mitogen-Activated Protein Kinase Kinases / Q000037:antagonists & inhibitors; D018919:Neovascularization, Physiologic / Q000235:genetics; D009570:Nitriles / Q000494:pharmacology; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000096:biosynthesis* / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis* / Q000378:metabolism; D014841:von Willebrand Factor / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: D002070:Butadienes; D050677:Core Binding Factor Alpha 3 Subunit; D004791:Enzyme Inhibitors; D009570:Nitriles; D019408:Platelet Endothelial Cell Adhesion Molecule-1; C493730:Runx3 protein, mouse; C113580:U 0126; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse; D014841:von Willebrand Factor; D048049:Extracellular Signal-Regulated MAP Kinases; D020929:Mitogen-Activated Protein Kinase Kinases