title: Molecular typing of human herpesvirus 8 isolates from patients with Kaposi's sarcoma in Hungary.
abstract: BACKGROUND
  Kaposi's sarcoma (KS) shows a distinct geographical and ethnic distribution. Genes at both ends of the human herpesvirus 8 (HHV-8) genome have been shown to vary considerably. Seven major molecular subtypes of HHV-8 were defined based on the amino acid sequence of the open reading frame K1 (orf-K1). The aim of the present study was to characterize HHV8 isolates from hospitalized patients in Hungary.
  
  
  METHODS
  A total of 36 archival paraffin-embedded Kaposi's sarcoma tissue samples were collected. Polymerase chain reaction (PCR) was carried out on the extracted DNA, using specific primers for HHV-8. After identifying the presence of HHV-8 by amplification of its orf26 region, the orf-K1 region was amplified, sequenced and used for phylogenetic analysis.
  
  
  RESULTS
  From the 36 orf26-positive cases, orf-K1 was amplified and was analyzed successfully in 12 cases. Phylogenetic studies, based on the complete K1 gene/protein sequences, indicate that all strains belong to the A subtype. Specifically, six of them were related to the A1 subgroup, six to the A2 subgroup and three previously reported to the A3 subgroup. Nucleotide sequence data are reported and are available in the Genbank database under accession numbers KF829938-KF829947.
authors: Department of Medical Microbiology, Medical and Health Science Center, University of Debrecen, 4032 Debrecen, Nagyerdei krt. 98., Hungary. konya@med.unideb.hu.
authors: Rita
authors: R
authors: Ötvös
authors: Attila
authors: A
authors: Juhasz
authors: Erika
authors: E
authors: Szalai
authors: Dorina
authors: D
authors: Ujvari
authors: Katalin
authors: K
authors: Ötvös
authors: Katalin
authors: K
authors: Szabo
authors: Eva
authors: E
authors: Remenyik
authors: Laszlo
authors: L
authors: Szekely
authors: Lajos
authors: L
authors: Gergely
authors: Jozsef
authors: J
authors: Konya
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D001483:Base Sequence; D004279:DNA, Viral / Q000097:blood / Q000235:genetics; D005260:Female; D019288:Herpesvirus 8, Human / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D006814:Hungary / Q000453:epidemiology; D008297:Male; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D058889:Molecular Typing; D016612:Paraffin Embedding; D010802:Phylogeny; D012514:Sarcoma, Kaposi / Q000097:blood / Q000453:epidemiology / Q000821:virology*; D016415:Sequence Alignment
chemical_list: D004279:DNA, Viral
keywords: HHV-8 genotypes; K1; Kaposi's sarcoma; PCR; subtype A