title: [Evaluation of anti-Pseudomonas aeruginosa specific secretory IgA antibodies in tears of patients with Pseudomonas aeruginosa keratitis].
abstract: OBJECTIVE
  To investigate the role of specific secretary IgA antibodies on lipopolysaccharide (LPS-sIgA) and exotoxin A (ETA-sIgA) in tear fluid produced by immunoresponse to P. aeruginosa.
  
  
  METHODS
  Subjects were divided into 3 groups; 41 eyes of 41 normal volunteers without a history of using contact lenses (CL) as controls, 32 eyes of 32 CL users without adverse events as a healthy CL wearer group (CL group), and 12 eyes of 12 patients with CL-related corneal ulcers from which P. aeruginosa was isolated (ulcer group). Tear fluid was obtained using filter paper, and the concentration of LPS-sIgA and ETA-sIgA in samples was determined using enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  The lower limit of concentration for LPS-sIgA and ETA-sIgA was 1.24 and 1.24 (U/mL), respectively. The positive eyes which exceeded this lower limit in LPS-sIgA were 17 of 22 eyes in the control group (median concentration 2.00U/ml, range 1.28 to 7.20), 15 of 25 eyes in the CL group (median 1.76 U/ml, range 1.24 to 6.92) and 1 of 12 eyes in the ulcer group (1.80 U/ml). The number of eyes which exceeded the lower limit in ETA-sIgA was 36 of 41 eyes in the control group (median concentration 6.56 U/ml, range 1.36 to 182), 28 of 32 eyes in the CL group (median 5.40 U/ml, range 1.56 to 29.20) and 5 of 12 eyes in the ulcer group (median 1.72 U/ml, range 1.40 to 2.16). There was no significant difference in LPS-sIgA and ETA-sIgA between the control group and the CL group, but they were significantly lower in the ulcer group than in the control group (p < 0.01, Steel's test).
  
  
  CONCLUSIONS
  Tear fluid in normal, healthy CL wearers contains LPS-sIgA and ETA-sIgA which acts as a barrier to P. aeruginosa infectious keratitis.
authors: Division of Ophthalmology, Department of Visual Sciences, Nihon University School of Medicine, Tokyo, Japan. nakashima.motohiro@nihon-u.ac.jp
authors: Motohiro
authors: M
authors: Nakashima
authors: Division of Ophthalmology, Department of Visual Sciences, Nihon University School of Medicine, Tokyo, Japan.
authors: Noriko
authors: N
authors: Inada
authors: Division of Ophthalmology, Department of Visual Sciences, Nihon University School of Medicine, Tokyo, Japan.
authors: Hiroshi
authors: H
authors: Kato
authors: Division of Ophthalmology, Department of Visual Sciences, Nihon University School of Medicine, Tokyo, Japan.
authors: Jun
authors: J
authors: Shoji
authors: Division of Ophthalmology, Department of Visual Sciences, Nihon University School of Medicine, Tokyo, Japan.
authors: Mitsuru
authors: M
authors: Sawa
mesh_terms: D000328:Adult; D003261:Contact Lenses / Q000009:adverse effects*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000032:analysis* / Q000276:immunology; D007634:Keratitis / Q000276:immunology* / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D013666:Tears / Q000276:immunology*
chemical_list: D007071:Immunoglobulin A, Secretory