title: Localization and distribution of wheat dwarf virus in its vector leafhopper, Psammotettix alienus.
abstract: Numerous virus pathogens are transmitted by specific arthropod vectors. Understanding the mechanism of transmission is a critical step in the epidemiology of plant viruses and is crucial for the development of effective disease control strategies. In this study, we describe the localization and distribution of Wheat dwarf virus (WDV), an economically important and widespread single-stranded DNA virus, in its leafhopper vector, Psammotettix alienus. The results suggest that WDV not only can move to the salivary glands from the anterior and middle midgut via the hemocoel but also can pass directly through the sheath of the filter chamber and be readily transmitted to healthy wheat plants within 5 min of an acquisition access period on infected plants. When a bacterial-expressed recombinant capsid protein (CP) was incubated with the internal organs of leafhoppers, CP-immunoreactive antigens were found at the anterior and middle midgut. Furthermore, when leafhoppers were fed with an antiserum raised against the CP, the accumulation of WDV in the gut cells, hemocoel, and salivary glands was significantly reduced. These data provide evidence that transmission of WDV is determined by a CP-mediated virion-vector retention mechanism.
authors: Yajiao
authors: Y
authors: Wang
authors: Qianzhuo
authors: Q
authors: Mao
authors: Wenwen
authors: W
authors: Liu
authors: ThiThi
authors: T
authors: Mar
authors: Taiyun
authors: T
authors: Wei
authors: Yan
authors: Y
authors: Liu
authors: Xifeng
authors: X
authors: Wang
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism; D017791:Geminiviridae / Q000502:physiology*; D006430:Hemiptera / Q000166:cytology / Q000821:virology*; D007303:Insect Vectors / Q000166:cytology / Q000821:virology*; D009758:Nymph; D010935:Plant Diseases / Q000821:virology*; D011994:Recombinant Proteins; D013997:Time Factors; D014908:Triticum / Q000821:virology*
chemical_list: D036022:Capsid Proteins; D011994:Recombinant Proteins