title: A novel teichuronic acid, the major polymer from the cell wall of Actinoplanes lobatus VKM Ac-676(T.).
abstract: The cell wall of Actinoplanes lobatus VKM Ac-676(T) (family Micromonosporaceae; order Actinomycetales; class Actinobacteria) contains two anionic glycopolymers. The major one is a teichuronic acid with a linear structure of the chain and heterogeneous repeating units, consisting of glucopyranose residues and statistically alternating residues of diaminouronic acids having the d-manno,l-gulo and d-gluco configurations in a ratio of 6:3:1, respectively. The teichuronic acid of this structure has not hitherto been found in Gram-positive bacteria. The minor glycopolymer is a teichoic acid of poly(glycosylpolyol phosphate) nature with the repeating unit -6)-[α-d-Manp(1→4)]-β-d-Galp-(1→2)-snGro-(3-P-. The structures of the glycopolymers were established by chemical methods and NMR spectroscopy.
authors: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect, 47, Moscow 119991, Russian Federation.
authors: Alexander S
authors: AS
authors: Shashkov
authors: School of Biology, M.V. Lomonosov Moscow State University, Moscow 119991, Russian Federation. Electronic address: streshinskaya@mail.ru.
authors: Galina M
authors: GM
authors: Streshinskaya
authors: School of Biology, M.V. Lomonosov Moscow State University, Moscow 119991, Russian Federation.
authors: Elena M
authors: EM
authors: Tul'skaya
authors: School of Biology, M.V. Lomonosov Moscow State University, Moscow 119991, Russian Federation.
authors: Yulia I
authors: YI
authors: Kozlova
authors: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect, 47, Moscow 119991, Russian Federation.
authors: Sof'ya N
authors: SN
authors: Senchenkova
authors: All-Russian Collection of Microorganisms (VKM), G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russian Federation.
authors: Ludmila I
authors: LI
authors: Evtushenko
mesh_terms: D000192:Actinomycetales / Q000737:chemistry; D000838:Anions / Q000737:chemistry; D002473:Cell Wall / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D011108:Polymers / Q000737:chemistry*; D011487:Protein Conformation; D014574:Uronic Acids / Q000737:chemistry*
chemical_list: D000838:Anions; D011108:Polymers; D014574:Uronic Acids; C010788:teichuronic acid
keywords: Actinoplanes lobatus; Anionic polysaccharides; Cell wall; NMR spectroscopy; Teichoic acid; Teichuronic acid