title: Hypoxic culture conditions for Mesenchymal Stromal/Stem Cells from Wharton's jelly: a critical parameter to consider in a therapeutic context.
abstract: Mesenchymal Stromal/Stem Cells from human Wharton's jelly (WJ-MSC) are an abundant and interesting source of stem cells for applications in cell and tissue engineering. Their fetal origin confers specific characteristics compared to Mesenchymal Stromal/Stem Cells isolated from human bone marrow (BM-MSC). The aim of this work was to optimize WJ-MSC culture conditions for their subsequent clinical use. We focused on the influence of oxygen concentration during monolayer expansion on several parameters to characterize MSC. Our work distinguished WJ-MSC from BM-MSC in terms of proliferation, telomerase activity and adipogenic differentiation. We also showed that hypoxia had a beneficial effect on proliferation potential, clonogenic capacity and to a lesser extent, on HLA-G expression of WJ-MSC during their expansion. Moreover, we reported for the first time an increase in chondrogenic differentiation when WJ-MSC were expanded under hypoxia. In an allogeneic therapeutic context, production of clinical batches requires generating high numbers of MSC whilst maintaining the cells' properties. Considering our results, hypoxia will be an important parameter to take into account. In addition, the clinical use of WJ-MSC would provide significant numbers of cells with maintenance of their proliferation and differentiation potential, particularly their chondrogenic potential. Due to their chondrogenic differentiation potential, WJ-MSC promise to be an interesting source of MSC for cell therapy or tissue engineering for cartilage repair and/or regeneration.
authors: Loic
authors: L
authors: Reppel
authors: Talar
authors: T
authors: Margossian
authors: Layale
authors: L
authors: Yaghi
authors: Philippe
authors: P
authors: Moreau
authors: Nathalie
authors: N
authors: Mercier
authors: Leonore
authors: L
authors: Leger
authors: Sebastien
authors: S
authors: Hupont
authors: Jean-Francois
authors: JF
authors: Stoltz
authors: Daniele
authors: D
authors: Bensoussan
authors: UMR 7365 CNRS-Universite de Lorraine, Ingenierie Moleculaire et Physiopathologie Articulaire (IMoPA), Biopole de l'Universite de Lorraine, Campus biologie-sante, Faculte de Medecine, Avenue de la Foret de Haye, BP 184, 54505 Vandoeuvre- les -Nancy, France. loic.reppel@univ-lorraine.fr.
authors: Celine
authors: C
authors: Huselstein
mesh_terms: D000943:Antigens, Differentiation / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002113:Calcification, Physiologic; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D010012:Osteogenesis; D044968:Regenerative Medicine; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014470:Umbilical Cord / Q000166:cytology; D059631:Wharton Jelly / Q000166:cytology
chemical_list: D000943:Antigens, Differentiation; D017931:DNA Primers