title: Grafting of a model protein on lactide and caprolactone based biodegradable films for biomedical applications.
abstract: Thermoplastic biodegradable polymers displaying elastomeric behavior and mechanical consistency are greatly appreciated for the regeneration of soft tissues and for various medical devices. However, while the selection of a suitable base material is determined by mechanical and biodegradation considerations, it is the surface properties of the biomaterial that are responsible for the biological response. In order to improve the interaction with cells and modulate their behavior, biologically active molecules can be incorporated onto the surface of the material. With this aim, the surface of a lactide and caprolactone based biodegradable elastomeric terpolymer was modified in two stages. First, the biodegradable polymer surface was aminated by atmospheric pressure plasma treatment and second a crosslinker was grafted in order to covalently bind the biomolecule. In this study, albumin was used as a model protein. According to X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM), albumin was efficiently immobilized on the surface of the terpolymer, the degree of albumin surface coverage (ΓBSA) reached ~35%. Moreover, gel permeation chromatography (GPC) studies showed that the hydrolytic degradation kinetic of the synthesized polymer was slightly delayed when albumin was grafted. However, the degradation process in the bulk of the material was unaffected, as demonstrated by Fourier transform infrared (FTIR) analyses. Furthermore, XPS analyses showed that the protein was still present on the surface after 28 days of degradation, meaning that the surface modification was stable, and that there had been enough time for the biological environment to interact with the modified material.
authors: Department of Mining-Metallurgy Engineering and Materials Science; POLYMAT; University of the Basque Country (UPV/EHU); School of Engineering; Bilbao, Spain; Laboratoire d'Ingénierie de Surface (LIS); Centre de recherche du CHU de Québec; Hôpital Saint-François d'Assise; Québec, QC Canada.
authors: Aitor
authors: A
authors: Larrañaga
authors: Department of Mining-Metallurgy Engineering and Materials Science; POLYMAT; University of the Basque Country (UPV/EHU); School of Engineering; Bilbao, Spain; Laboratoire d'Ingénierie de Surface (LIS); Centre de recherche du CHU de Québec; Hôpital Saint-François d'Assise; Québec, QC Canada.
authors: Andrée-Anne
authors: AA
authors: Guay-Bégin
authors: Laboratoire d'Ingénierie de Surface (LIS); Centre de recherche du CHU de Québec; Hôpital Saint-François d'Assise; Québec, QC Canada; Département de génie des mines, de la métallurgie et des matériaux; Centre de Recherche sur les Matériaux Avancés (CERMA); Université Laval; Québec, QC Canada.
authors: Pascale
authors: P
authors: Chevallier
authors: Laboratoire d'Ingénierie de Surface (LIS); Centre de recherche du CHU de Québec; Hôpital Saint-François d'Assise; Québec, QC Canada; Département de génie des mines, de la métallurgie et des matériaux; Centre de Recherche sur les Matériaux Avancés (CERMA); Université Laval; Québec, QC Canada.
authors: Gad
authors: G
authors: Sabbatier
authors: Department of Mining-Metallurgy Engineering and Materials Science; POLYMAT; University of the Basque Country (UPV/EHU); School of Engineering; Bilbao, Spain.
authors: Jorge
authors: J
authors: Fernández
authors: Laboratoire d'Ingénierie de Surface (LIS); Centre de recherche du CHU de Québec; Hôpital Saint-François d'Assise; Québec, QC Canada; Département de génie des mines, de la métallurgie et des matériaux; Centre de Recherche sur les Matériaux Avancés (CERMA); Université Laval; Québec, QC Canada.
authors: Gaétan
authors: G
authors: Laroche
authors: Department of Mining-Metallurgy Engineering and Materials Science; POLYMAT; University of the Basque Country (UPV/EHU); School of Engineering; Bilbao, Spain.
authors: Jose-Ramon
authors: JR
authors: Sarasua
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D063566:Biodegradable Plastics / Q000737:chemistry*; D020811:Biomedical Technology; D002152:Calorimetry, Differential Scanning; D002850:Chromatography, Gel; D018625:Microscopy, Atomic Force; D056951:Photoelectron Spectroscopy; D011091:Polyesters / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D063566:Biodegradable Plastics; D011091:Polyesters; C062968:lactide-caprolactone copolymer
keywords: albumin; grafting; hydrolytic degradation; poly(lactide/ε-caprolactone); surface modification; terpolymer