title: A genetic approach to study H2O2 scavenging in fission yeast--distinct roles of peroxiredoxin and catalase.
abstract: The main peroxiredoxin in Schizosaccharomyces pombe, Tpx1, is important to sustain aerobic growth, and cells lacking this protein are only able to grow on solid plates under anaerobic conditions. We have found that deletion of the gene coding for thioredoxin reductase, trr1, is a suppressor of the sensitivity to aerobic growth of Δtpx1 cells, so that cells lacking both proteins are able to grow on solid plates in the presence of oxygen. We have investigated this suppression effect, and determined that it depends on the presence of catalase, which is constitutively expressed in Δtrr1 cells in a transcription factor Pap1-dependent manner. A complete characterization of the repertoire of hydrogen peroxide scavenging activities in fission yeast suggests that Tpx1 is the only enzyme with sufficient sensitivity for peroxides and cellular abundance as to control the low levels produced during aerobic growth, catalase being the next barrier of detoxification when the steady-state levels of peroxides are increased in Δtpx1 cells. Gpx1, the only glutathione peroxidase encoded by the S. pombe genome, only has a minor secondary role when extracellular peroxides are added. Our study proposes non-overlapping roles for the different hydrogen peroxide scavenging activities of this eukaryotic organism.
authors: Oxidative Stress and Cell Cycle Group, Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, C/ Dr. Aiguader 88, E-08003, Barcelona, Spain.
authors: Esther
authors: E
authors: Paulo
authors: Sarela
authors: S
authors: García-Santamarina
authors: Isabel A
authors: IA
authors: Calvo
authors: Mercè
authors: M
authors: Carmona
authors: Susanna
authors: S
authors: Boronat
authors: Alba
authors: A
authors: Domènech
authors: José
authors: J
authors: Ayté
authors: Elena
authors: E
authors: Hidalgo
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D050976:Basic-Leucine Zipper Transcription Factors / Q000378:metabolism; D002374:Catalase / Q000378:metabolism*; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D006861:Hydrogen Peroxide / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D000073718:Pancreatitis-Associated Proteins; D054464:Peroxiredoxins / Q000235:genetics / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D013880:Thioredoxin-Disulfide Reductase / Q000235:genetics
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; D000073718:Pancreatitis-Associated Proteins; C105096:Pap1 protein, S pombe; C000612141:REG3A protein, human; D029702:Schizosaccharomyces pombe Proteins; C515985:Tpx1 protein, S pombe; D006861:Hydrogen Peroxide; D054464:Peroxiredoxins; D002374:Catalase; D013880:Thioredoxin-Disulfide Reductase; D010100:Oxygen