title: Quantitative PCR for detection of mRNA and gDNA in environmental isolates.
abstract: Quantitative PCR is used to gauge the abundance of specific nucleic acid species within purified samples. Due to its high sensitivity and minimal operation costs, this method is routinely applied in modern molecular bioscience laboratories. Nonetheless, all quantitative PCR experiments must include several carefully designed, yet simple, controls to ensure the reliability of the analyses. The aim of this chapter is to provide basic quantitative PCR methods, from primer design through data analysis, that are generally applicable to studies in microbiology. These methods allow the abundance of targeted RNA or DNA molecules to be determined in nucleic acid samples purified from a variety of biological sources.
authors: School of Biological Sciences, University of Sydney, Sydney, NSW, Australia.
authors: Anthony J
authors: AJ
authors: Brzoska
authors: Karl A
authors: KA
authors: Hassan
mesh_terms: D004247:DNA; D004783:Environmental Microbiology; D005819:Genetic Markers; D012333:RNA, Messenger; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods / Q000592:standards
chemical_list: D005819:Genetic Markers; D012333:RNA, Messenger; D004247:DNA