title: RNA aptamer against autoantibodies associated with multiple sclerosis and bioluminescent detection probe on its basis.
abstract: Nowadays, there are no specific laboratory tests for establishing the diagnosis of multiple sclerosis (MS). The presence of proteolytic autoantibodies against myelin basic protein is now considered as a characteristic feature of MS. New 2'-F-containing RNA aptamer of high affinity and specificity to these antibodies was selected. Covalent conjugate of this aptamer and Ca(2+)-regulated photoprotein obelin was obtained for the first time and applied as a label in bioluminescent microplate assay to detect target antibodies. The developed model solid-phase microassay is simple, fast, and highly sensitive.
authors: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences , Novosibirsk 630090, Russia.
authors: Maria A
authors: MA
authors: Vorobjeva
authors: Vasilisa V
authors: VV
authors: Krasitskaya
authors: Alesya A
authors: AA
authors: Fokina
authors: Valentina V
authors: VV
authors: Timoshenko
authors: Georgy A
authors: GA
authors: Nevinsky
authors: Alya G
authors: AG
authors: Venyaminova
authors: Ludmila A
authors: LA
authors: Frank
mesh_terms: D000293:Adolescent; D000328:Adult; D052157:Aptamers, Nucleotide / Q000276:immunology*; D001323:Autoantibodies / Q000276:immunology*; D005260:Female; D006801:Humans; D049449:Luminescence; D008297:Male; D008875:Middle Aged; D015335:Molecular Probes; D009103:Multiple Sclerosis / Q000276:immunology*; D055815:Young Adult
chemical_list: D052157:Aptamers, Nucleotide; D001323:Autoantibodies; D015335:Molecular Probes