title: Resveratrol induces human K562 cell apoptosis, erythroid differentiation, and autophagy.
abstract: Resveratrol (Res) is a naturally occurring phytoalexin with apoptotic and inducing-glob effects in leukemic cells, but the potential induction of erythroid differentiation in cells is not fully understood. Here, we investigated the effects of Res on human erythro-megakaryoblastic leukemia cell line K562. Among the treated cells, proliferation was inhibited and the occurrence of cell apoptosis and cell death were detected. Erythroid differentiation assay was explored, and we found that Res could increase the expression of glycophorin A (GPA), HBA1, HBB, and γ-globin genes and enforced the expression of GPA, CD71, and Band3 proteins. Res also induced K562 cell autophagy when the concentration of Res was increased up to 50 or 100 μM. Our findings suggested that Res possesses the potency not only inducing apoptosis but also inducing erythroid differentiation and autophagy in K562 cells. These results provide that Res may be a therapeutic candidate for chronic myelogenous leukemia treatment.
authors: Molecular Biology Research Center, School of Life Science, Central South University, Changsha, 410078, China.
authors: Hui-Wen
authors: HW
authors: Yan
authors: Wei-Xin
authors: WX
authors: Hu
authors: Jie-Ying
authors: JY
authors: Zhang
authors: Ye
authors: Y
authors: Wang
authors: Kun
authors: K
authors: Xia
authors: Min-Yuan
authors: MY
authors: Peng
authors: Jing
authors: J
authors: Liu
mesh_terms: D001457:Anion Exchange Protein 1, Erythrocyte / Q000032:analysis; D015703:Antigens, CD / Q000032:analysis; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001343:Autophagy / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation; D041905:Erythroid Cells / Q000166:cytology / Q000187:drug effects*; D006021:Glycophorins / Q000032:analysis; D006801:Humans; D020014:K562 Cells; D011990:Receptors, Transferrin / Q000032:analysis; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D001457:Anion Exchange Protein 1, Erythrocyte; D015703:Antigens, CD; D000970:Antineoplastic Agents; C068126:CD71 antigen; C409972:GYPA protein, human; D006021:Glycophorins; D011990:Receptors, Transferrin; D013267:Stilbenes; D000077185:Resveratrol