title: Next generation barcode tagged sequencing for monitoring microbial community dynamics.
abstract: Microbial identification using 16S rDNA variable regions has become increasingly popular over the past decade. The application of next-generation amplicon sequencing to these regions allows microbial communities to be sequenced in far greater depth than previous techniques, as well as allowing for the identification of unculturable or rare organisms within a sample. Multiplexing can be used to sequence multiple samples in tandem through the use of sample-specific identification sequences which are attached to each amplicon, making this a cost-effective method for large-scale microbial identification experiments.
authors: Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW, Australia.
authors: Katy
authors: K
authors: Breakwell
authors: Sasha G
authors: SG
authors: Tetu
authors: Liam D H
authors: LD
authors: Elbourne
mesh_terms: D019295:Computational Biology / Q000379:methods; D058893:DNA Barcoding, Taxonomic; D004783:Environmental Microbiology; D059014:High-Throughput Nucleotide Sequencing; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D012336:RNA, Ribosomal, 16S