title: Hormone profiling and transcription analysis reveal a major role of ABA in tomato salt tolerance.
abstract: The response and adaptation of plants to different environmental stresses are of great interest as they provide the key to understanding the mechanisms underlying stress tolerance. In this study, the changing patterns of four endogenous hormones and various physiological and biochemical parameters of both a salt-tolerant (LA2711) and a salt-sensitive (ZS-5) tomato cultivar were examined under salt stress and non-stress conditions. Additionally, the transcription of key genes in the abscisic acid (ABA) biosynthesis and metabolism were analyzed at different time points. The results indicated that gene expression responsible for ABA biosynthesis and metabolism coincided with the hormone level, and SlNCED1 and SlCYP707A3 may play major roles in the process. LA2711 performed superior to ZS-5 on various parameters, including seed germination, Na(+) compartmentation, selective absorption of K(+), and antioxidant enzymes activity. The difference in salt tolerance between the two genotypes could be attributed to the different levels of ABA due to differences in gene expression of key genes in ABA biosynthesis and metabolism. Although gibberellin, cytokinin and auxin were involved, our results indicated that ABA signaling plays a major role in tomato salt tolerance. As compared to ZS-5, LA2711 had a higher capability to selectively absorb and redistribute K(+) and a higher tolerance to Na(+) in young leaves, which may be the main physiological mechanisms of salt tolerance.
authors: College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, PR China.
authors: Rongchao
authors: R
authors: Yang
authors: College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, PR China.
authors: Ting
authors: T
authors: Yang
authors: College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, PR China.
authors: Haijun
authors: H
authors: Zhang
authors: College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, PR China.
authors: Yan
authors: Y
authors: Qi
authors: College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, PR China.
authors: Yanxia
authors: Y
authors: Xing
authors: College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, PR China.
authors: Na
authors: N
authors: Zhang
authors: College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, PR China.
authors: Ren
authors: R
authors: Li
authors: School of Agriculture, Virginia State University, PO Box 9061, Petersburg, VA 23806, USA.
authors: Sarah
authors: S
authors: Weeda
authors: School of Agriculture, Virginia State University, PO Box 9061, Petersburg, VA 23806, USA.
authors: Shuxin
authors: S
authors: Ren
authors: College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, PR China. Electronic address: bouy@mail.hzau.edu.cn.
authors: Bo
authors: B
authors: Ouyang
authors: College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, PR China. Electronic address: yaguo@cau.edu.cn.
authors: Yang-Dong
authors: YD
authors: Guo
mesh_terms: D000040:Abscisic Acid / Q000235:genetics* / Q000378:metabolism; D000975:Antioxidants / Q000378:metabolism; D015870:Gene Expression; D020869:Gene Expression Profiling; D017343:Genes, Plant; D005838:Genotype; D018525:Germination / Q000235:genetics; D018551:Solanum lycopersicum / Q000235:genetics* / Q000378:metabolism; D010937:Plant Growth Regulators / Q000235:genetics* / Q000378:metabolism; D018515:Plant Leaves; D011188:Potassium / Q000378:metabolism; D055049:Salt Tolerance / Q000235:genetics*; D012964:Sodium / Q000378:metabolism*; D012965:Sodium Chloride / Q000378:metabolism*; D013045:Species Specificity; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D000975:Antioxidants; D010937:Plant Growth Regulators; D012965:Sodium Chloride; D000040:Abscisic Acid; D012964:Sodium; D011188:Potassium
keywords: Gene expression; Hormones; Physiological and biochemical; Salinity stress; Tomato