title: Detection of Mycobacterium avium subspecies paratuberculosis in patients with Crohn's disease is unrelated to the presence of single nucleotide polymorphisms rs2241880 (ATG16L1) and rs10045431 (IL12B).
abstract: Mycobacterium avium subspecies paratuberculosis (MAP) has been controversially linked with Crohn's disease (CD). Detection of MAP in CD has been highly variable, and one explanation might be the genetic heterogeneity of this syndrome. Many of the single nucleotide polymorphisms (SNPs) linked with CD are contained within genes that are associated with bacterial handling in general, and some are specifically implicated in susceptibility to mycobacterial disease. We tested a cohort of IBD patients (n = 149) to determine whether the presence of MAP was associated with a selection of these SNPs. Blood samples from CD patients (n = 84), ulcerative colitis (UC, n = 65) patients and healthy controls (n = 55) were examined for the presence of MAP and SNPs in ATG16L1, IL12B, NOD2/CARD15, NKx2-3, IL23R and IRGM. Statistical analysis was then used to determine whether there was any association between the presence of MAP and these SNPs. MAP, rs2241880 (ATG16L1) and rs10045431 (IL12B) were found to be significantly associated with CD. The presence of MAP was not related to the status of the SNPs in ATG16L1 or IL12B. We have found no evidence for the contribution of these SNPs to the presence of MAP in CD patients.
authors: Department of Microbiology, University College Cork, Cork, Ireland.
authors: James P
authors: JP
authors: Dalton
authors: Alan
authors: A
authors: Desmond
authors: Fergus
authors: F
authors: Shanahan
authors: Colin
authors: C
authors: Hill
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000071183:Autophagy-Related Proteins; D002352:Carrier Proteins / Q000235:genetics*; D003424:Crohn Disease / Q000150:complications / Q000235:genetics* / Q000382:microbiology; D005260:Female; D056726:Genetic Association Studies; D006801:Humans; D053762:Interleukin-12 Subunit p40 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016927:Mycobacterium avium subsp. paratuberculosis / Q000302:isolation & purification*; D010283:Paratuberculosis / Q000150:complications / Q000235:genetics* / Q000382:microbiology; D020641:Polymorphism, Single Nucleotide; D055815:Young Adult
chemical_list: C518959:ATG16L1 protein, human; D000071183:Autophagy-Related Proteins; D002352:Carrier Proteins; C500879:IL12B protein, human; D053762:Interleukin-12 Subunit p40