title: Ganoderma atrum polysaccharide improves aortic relaxation in diabetic rats via PI3K/Akt pathway.
abstract: A newly identified polysaccharide (PSG-1) has been purified from Ganoderma atrum. The study was to investigate the protective effect of PSG-1 on diabetes-induced endothelial dysfunction in rat aorta. Rats were fed a high fat diet for 8 weeks and then injected with a low dose of streptozotocin to induce type 2 diabetes. The diabetic rats were orally treated with PSG-1 for 4 weeks. It was found that administration of PSG-1 significantly reduced levels of fasting blood glucose, improved endothelium-dependent aortic relaxation, increased levels of phosphoinositide 3-kinase (PI3K), phospho-Akt (p-Akt), endothelial nitric oxide synthase (eNOS) and nitric oxide in the aorta from diabetic rats, compared to un-treated diabetics. These results suggested that the protective effects of PSG-1 against endothelial dysfunction may be related to activation of the PI3K/Akt/eNOS pathway.
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors: Ke-Xue
authors: KX
authors: Zhu
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address: spnie@ncu.edu.cn.
authors: Shao-Ping
authors: SP
authors: Nie
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors: Chuan
authors: C
authors: Li
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland, New Zealand.
authors: Deming
authors: D
authors: Gong
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors: Ming-Yong
authors: MY
authors: Xie
mesh_terms: D000818:Animals; D001011:Aorta / Q000187:drug effects* / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000378:metabolism; D032902:Ganoderma / Q000737:chemistry*; D008297:Male; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D051058:Oncogene Protein v-akt / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D011134:Polysaccharides / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D052250:Nitric Oxide Synthase Type III; D051058:Oncogene Protein v-akt; D019869:Phosphatidylinositol 3-Kinases; D011134:Polysaccharides
keywords: Aorta; Ganoderma atrum polysaccharide; Vascular endothelium; Vasorelaxation