title: Ancient whole genome enrichment using baits built from modern DNA.
abstract: We report metrics from complete genome capture of nuclear DNA from extinct mammoths using biotinylated RNAs transcribed from an Asian elephant DNA extract. Enrichment of the nuclear genome ranged from 1.06- to 18.65-fold, to an apparent maximum threshold of ∼80% on-target. This projects an order of magnitude less costly complete genome sequencing from long-dead organisms, even when a reference genome is unavailable for bait design.
authors: McMaster Ancient DNA Centre, Department of Anthropology, McMaster University, Hamilton, ON, Canada.
authors: Jacob M
authors: JM
authors: Enk
authors: Alison M
authors: AM
authors: Devault
authors: Melanie
authors: M
authors: Kuch
authors: Yusuf E
authors: YE
authors: Murgha
authors: Jean-Marie
authors: JM
authors: Rouillard
authors: Hendrik N
authors: HN
authors: Poinar
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004606:Elephants / Q000235:genetics; D005580:Fossils; D016678:Genome; D023281:Genomics / Q000379:methods*; D049690:History, Ancient; D056665:Mammoths / Q000235:genetics*; D016415:Sequence Alignment / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D004247:DNA
keywords: ancient DNA; targeted enrichment; whole-genome capture