title: Phytochemical screening and in-vitro antioxidant activity isolated bioactive compounds from Tridax procumbens Linn.
abstract: Tridax procumbens L., Asteraceae, has been extensively used for various ailments in the Ayurvedic system of medicine. Previous studies have revealed remarkable phytoconstituents from Tridax procumbens L. with significant antioxidant activity. The aim of the present study is to measure the anti-DPPH activity of the purified isolated compounds from n butanol soluble part and ethyl acetate soluble part of successive methanolic extract of Tridax procumbens L. We thus quantified the total phenolic and total flavonoids in different purified isolated compounds, the whole of the tests were evaluated with a sample cone. of 100 microg mL(-1) and were determined spectrophotometrically using Folin-ciocaltue and AlCl3 reagents, respectively. DPPH (1,1-diphenyl, 2-picryl hydrazyl) assay was used to determine the in vitro antioxidant activity of different isolated compounds. Isolated compounds, one from ethyl acetate soluble part (EF-I) and one from n butanol soluble part (BF-II) were reported to possess a significant anti DPPH activity with lowest IC50 values 67.26 and 80.90 microg mL(-1), respectively while comparable to standard ascorbic acid with IC50 value of 59.62 microg mL(-1), due to the high concentration of phenols 146.4 microg mL(-1) from EF-I and 142.2 microg mL(-1) from BF-II and flavonoids 48 and 42.5 microg mL(-1) found in EF-I and BF-II isolated compounds, respectively.
authors: P.G. Department of Botany, Govt. Maharaja Autonomous P.G. College, Chhatarpur-471405, M.P, India.
authors: Manjusha
authors: M
authors: Saxena
authors: Pest Control and Ayurvedic Drug Research Laboratory, S.S.L. Jain P.G. College, Vidisha-464001, M.P. India.
authors: Abrar Hussain
authors: AH
authors: Mir
authors: P.G. Department of Zoology, Bhoj Mahavidhyalya Bhopal M.P, India.
authors: Manik
authors: M
authors: Sharma
authors: P.G. Department of Zoology, Bhoj Mahavidhyalya Bhopal M.P, India.
authors: Mohd Yousu
authors: MY
authors: Malla
authors: Pest Control and Ayurvedic Drug Research Laboratory, S.S.L. Jain P.G. College, Vidisha-464001, M.P. India.
authors: Sumeerah
authors: S
authors: Qureshi
authors: P.G. Department of Zoology, Bhoj Mahavidhyalya Bhopal M.P, India.
authors: Mohd Iqbal
authors: MI
authors: Mir
authors: P.G. Department of Botany, Govt. Maharaja Autonomous P.G. College, Chhatarpur-471405, M.P, India.
authors: Yogesh
authors: Y
authors: Chaturvedi
mesh_terms: D020001:1-Butanol / Q000737:chemistry; D000085:Acetates / Q000737:chemistry; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification*; D019659:Asteraceae / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000737:chemistry; D005419:Flavonoids / Q000302:isolation & purification; D000432:Methanol / Q000737:chemistry; D010636:Phenols / Q000302:isolation & purification; D008517:Phytotherapy; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D057685:Plant Weeds / Q000737:chemistry*; D010946:Plants, Medicinal; D012997:Solvents / Q000737:chemistry; D013053:Spectrophotometry
chemical_list: D000085:Acetates; D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D012997:Solvents; C007650:ethyl acetate; D020001:1-Butanol; C004931:1,1-diphenyl-2-picrylhydrazyl; D000432:Methanol