title: Chemical composition of the bark of Tetrapterys mucronata and identification of acetylcholinesterase inhibitory constituents.
abstract: The secondary metabolite content of Tetrapterys mucronata, a poorly studied plant that is used occasionally in Brazil for the preparation of a psychotropic plant decoction called "Ayahuasca", was determined to establish its chemical composition and to search for acetylcholinesterase (AChE) inhibitors. The ethanolic extract of the bark of T. mucronata exhibited in vitro AChE inhibition in a TLC bioautography assay. To localize the active compounds, biological profiling for AChE inhibition was performed using at-line HPLC-microfractionation in 96-well plates and subsequent AChE inhibition bioautography. The analytical HPLC-PDA conditions were transferred geometrically to a preparative medium-pressure liquid chromatography column using chromatographic calculations for the efficient isolation of the active compounds at the milligram scale. Twenty-two compounds were isolated, of which six are new natural products. The structures of the new compounds (9, 10, 16-18, and 20) were elucidated by spectroscopic data interpretation. Compounds 1, 5, 6, 9, and 10 inhibited AChE with IC50 values below 15 μM.
authors: Núcleo de Bioensaios, Biossíntese e Ecofisiologia de Produtos Naturais, NuBBE, Instituto de Química, Universidade Estadual Paulista (UNESP) , Araraquara, São Paulo, Brazil.
authors: Marcos Marçal Ferreira
authors: MM
authors: Queiroz
authors: Emerson Ferreira
authors: EF
authors: Queiroz
authors: Maria Luiza
authors: ML
authors: Zeraik
authors: Samad Nejad
authors: SN
authors: Ebrahimi
authors: Laurence
authors: L
authors: Marcourt
authors: Muriel
authors: M
authors: Cuendet
authors: Ian
authors: I
authors: Castro-Gamboa
authors: Matthias
authors: M
authors: Hamburger
authors: Vanderlan
authors: V
authors: da Silva Bolzani
authors: Jean-Luc
authors: JL
authors: Wolfender
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D001688:Biological Products / Q000737:chemistry / Q000302:isolation & purification*; D001938:Brazil; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D007211:Indoles / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D029581:Malpighiaceae / Q000737:chemistry*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010616:Phenanthrenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology
chemical_list: D001688:Biological Products; D002800:Cholinesterase Inhibitors; D007211:Indoles; D010616:Phenanthrenes; D010936:Plant Extracts; D000110:Acetylcholinesterase