title: Dually fluorescent core-shell microgels for ratiometric imaging in live antigen-presenting cells.
abstract: Core-shell microgels containing sensors/dyes in a matrix were fabricated by two-stage free radical precipitation polymerization method for ratiometric sensing/imaging. The microgels composing of poly(N-isopropylacrylamide) (PNIPAm) shell exhibits a low critical solution temperature (LCST), underwent an entropically driven transition from a swollen state to a deswollen state, which exhibit a hydrodynamic radius of ∼ 450 nm at 25 °C (in vitro) and ∼ 190 nm at 37 °C (in vivo). The microgel's ability of escaping from lysosome into cytosol makes the microgel be a potential candidate for cytosolic delivery of sensors/probes. Non-invasive imaging/sensing in Antigen-presenting cells (APCs) was feasible by monitoring the changes of fluorescence intensity ratios. Thus, these biocompatible microgels-based imaging/sensing agents may be expected to expand current molecular imaging/sensing techniques into methods applicable to studies in vivo, which could further drive APC-based treatments.
authors: Center for Biosignatures Discovery Automation, Biodesign Institute, Arizona State University, Tempe, Arrizona, United States of America.
authors: Xianfeng
authors: X
authors: Zhou
authors: Center for Biosignatures Discovery Automation, Biodesign Institute, Arizona State University, Tempe, Arrizona, United States of America.
authors: Fengyu
authors: F
authors: Su
authors: Center for Biosignatures Discovery Automation, Biodesign Institute, Arizona State University, Tempe, Arrizona, United States of America.
authors: Yanqing
authors: Y
authors: Tian
authors: Center for Biosignatures Discovery Automation, Biodesign Institute, Arizona State University, Tempe, Arrizona, United States of America.
authors: Deirdre R
authors: DR
authors: Meldrum
mesh_terms: D000180:Acrylic Resins / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D000938:Antigen-Presenting Cells / Q000378:metabolism / Q000502:physiology*; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism; D002460:Cell Line; D003600:Cytosol / Q000378:metabolism; D005453:Fluorescence; D005782:Gels / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism / Q000502:physiology; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D058105:Polymerization; D013696:Temperature
chemical_list: D000180:Acrylic Resins; D001672:Biocompatible Materials; D005782:Gels; C052970:poly-N-isopropylacrylamide