title: Characterization of 15 STR cannabis loci: nomenclature proposal and SNPSTR haplotypes.
abstract: The standardization of methods for individualizing Cannabis sativa plants could offer new possibilities in the investigation of its illegal trade. Here we present the first nomenclature proposal for 15 cannabis STRs, which allows an initial standardization for performing comparisons between laboratories and generating genotype databases. Several alleles of the 15 STR loci have been sequenced. This has revealed that not all the STR loci are equally suitable for the individualization purposes. Moreover, several nucleotide variations have been detected both inside the repeat structure and/or in the flanking region. All the different SNPSTR haplotypes are presented and compared with the previous sequence raw data of the 15 STR loci. The SNPSTR data could considerably increase the informative value of the STRs, which could be very useful in complex cases.
authors: Institute of Legal Medicine, Münster University, Germany; BIOMICs Research Group, Lascaray Research Center, University of the Basque Country UPV/EHU, Vitoria-Gasteiz, Spain.
authors: Laura
authors: L
authors: Valverde
authors: Institute of Legal Medicine, Münster University, Germany.
authors: Christian
authors: C
authors: Lischka
authors: Institute of Legal Medicine, Münster University, Germany.
authors: Stefanie
authors: S
authors: Scheiper
authors: Institute of Legal Medicine, Münster University, Germany.
authors: Johanna
authors: J
authors: Nedele
authors: Institute of Legal Medicine, Münster University, Germany.
authors: Rachel
authors: R
authors: Challis
authors: BIOMICs Research Group, Lascaray Research Center, University of the Basque Country UPV/EHU, Vitoria-Gasteiz, Spain.
authors: Marian M
authors: MM
authors: de Pancorbo
authors: Institute of Legal Medicine, Münster University, Germany.
authors: Heidi
authors: H
authors: Pfeiffer
authors: Institute of Legal Medicine, Münster University, Germany. Electronic address: Stephan.Koehnemann@ukmuenster.de.
authors: Stephan
authors: S
authors: Köhnemann
mesh_terms: D000483:Alleles; D002188:Cannabis / Q000235:genetics*; D016172:DNA Fingerprinting; D056426:Genetic Loci; D006239:Haplotypes; D018895:Microsatellite Repeats; D060885:Multiplex Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D009626:Terminology as Topic
keywords: Cannabis; Forensic genetics; Nomenclature; SNPSTR haplotype; STR