title: Resveratrol improves bone repair by modulation of bone morphogenetic proteins and osteopontin gene expression in rats.
abstract: This study investigated the effect of resveratrol on bone healing and its influence on the gene expression of osteogenic markers. Two calvarial defects were created and one screw-shaped titanium implant was inserted in the tibia of rats that were assigned to daily administration of placebo (control group, n=15) or 10mg/kg of resveratrol (RESV group, n=15) for 30 days. The animals were then sacrificed. One of the calvarial defects was processed for histomorphometric analysis and the tissue relative to the other was collected for mRNA quantification of bone morphogenetic protein (BMP)-2, BMP-7, osteopontin (OPN), bone sialoprotein (BSP), osteoprotegrin (OPG), and receptor activator of NF-κB ligand (RANKL). Implants were removed by applying a counter-torque force. Histomorphometric analysis revealed higher remaining defect in the calvarial defects of the control group than the RESV group (P=0.026). Resveratrol increased the counter-torque values of implant removal when compared to control therapy (P=0.031). Gene expression analysis showed a higher expression of BMP-2 (P=0.011), BMP-7 (P=0.049), and OPN (P=0.002) genes in the RESV group than in the control group. In conclusion, resveratrol improved the repair of critical-sized bone defects and the biomechanical retention of implants. Indeed, this natural agent may up-regulate the gene expression of important osteogenic markers.
authors: Dental Research Division, School of Dentistry, Paulista University, São Paulo, SP, Brazil.
authors: R C
authors: RC
authors: Casarin
authors: Dental Research Division, School of Dentistry, Paulista University, São Paulo, SP, Brazil.
authors: M Z
authors: MZ
authors: Casati
authors: Dental Research Division, School of Dentistry, Paulista University, São Paulo, SP, Brazil.
authors: S P
authors: SP
authors: Pimentel
authors: Dental Research Division, School of Dentistry, Paulista University, São Paulo, SP, Brazil.
authors: F R
authors: FR
authors: Cirano
authors: Dental Research Division, School of Dentistry, Paulista University, São Paulo, SP, Brazil.
authors: M
authors: M
authors: Algayer
authors: Dental Research Division, School of Dentistry, Paulista University, São Paulo, SP, Brazil.
authors: P R
authors: PR
authors: Pires
authors: Dental Research Division, School of Dentistry, Paulista University, São Paulo, SP, Brazil.
authors: B
authors: B
authors: Ghiraldini
authors: Department of Periodontology, Dental Research Division, Guarulhos University, Guarulhos, SP, Brazil.
authors: P M
authors: PM
authors: Duarte
authors: Dental Research Division, School of Dentistry, Paulista University, São Paulo, SP, Brazil. Electronic address: fernanda@ribbeiro.com.
authors: F V
authors: FV
authors: Ribeiro
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics*; D055419:Bone Morphogenetic Protein 7 / Q000235:genetics*; D015921:Dental Implants; D015870:Gene Expression; D020007:Implants, Experimental; D058574:Integrin-Binding Sialoprotein / Q000235:genetics; D008297:Male; D053495:Osteopontin / Q000378:metabolism*; D053244:Osteoprotegerin / Q000235:genetics; D053245:RANK Ligand / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013977:Tibia / Q000601:surgery*; D014025:Titanium; D015854:Up-Regulation; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D055396:Bone Morphogenetic Protein 2; D055419:Bone Morphogenetic Protein 7; D015921:Dental Implants; D058574:Integrin-Binding Sialoprotein; D053244:Osteoprotegerin; D053245:RANK Ligand; D012333:RNA, Messenger; D013267:Stilbenes; D053495:Osteopontin; D014025:Titanium; D000077185:Resveratrol
keywords: Gene expression; Medicinal; Osseointegration; Plants; Rats; Resveratrol; Wound healing