title: Astroglial-derived periostin promotes axonal regeneration after spinal cord injury.
abstract: Traumatic spinal cord injury (SCI) results in a cascade of tissue responses leading to cell death, axonal degeneration, and glial scar formation, exacerbating the already hostile environment and further inhibiting axon regeneration. Overcoming these inhibitory cues and promoting axonal regeneration is one of the primary targets in developing a cure for SCI. Previously, we demonstrated that transplantation of bone morphogenetic protein (BMP)-induced astrocytes derived from embryonic glial-restricted precursors (GDAs(BMP)) promotes extensive axonal growth and motor function recovery in a rodent spinal cord injury model. Here, we identify periostin (POSTN), a secreted protein, as a key component of GDA(BMP)-induced axonal regeneration. POSTN is highly expressed by GDAs(BMP) and the perturbation of POSTN expression by shRNA diminished GDA(BMP)-induced neurite extension in vitro. We also found that recombinant POSTN is sufficient to overcome the inhibitory effect of scar-associated molecules and promote neurite extension in vitro by signaling through focal adhesion kinase and Akt. Furthermore, transplantation of POSTN-deficient GDAs(BMP) into the injured rat spinal cord resulted in compromised axonal regeneration, indicating that POSTN plays an essential role in GDA(BMP)-mediated axonal regeneration. This finding reveals not only one of the major mechanisms underlying GDA(BMP)-dependent recovery from SCI, but also the potential of POSTN as a therapeutic agent for traumatic injury of the CNS.
authors: Stem Cell and Regenerative Medicine Institute, Department of Biomedical Genetics, and Department of Pathology, University of Rochester Medical Center, Rochester, New York 14642.
authors: Chung-Hsuan
authors: CH
authors: Shih
authors: Michelle
authors: M
authors: Lacagnina
authors: Kelly
authors: K
authors: Leuer-Bisciotti
authors: Christoph
authors: C
authors: Pröschel
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism* / Q000637:transplantation*; D001369:Axons / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology; D004195:Disease Models, Animal; D053595:Embryonic Stem Cells / Q000166:cytology; D009416:Nerve Regeneration / Q000502:physiology*; D058953:Neural Stem Cells / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000378:metabolism*
chemical_list: D015815:Cell Adhesion Molecules; C512836:Postn protein, rat
keywords: astrocyte; cell therapy; glial precursor; neurite outgrowth; spinal cord; support cell