title: Ultrasensitive label-free detection of PNA-DNA hybridization by reduced graphene oxide field-effect transistor biosensor.
abstract: A reduced graphene oxide (R-GO)-based field-effect transistor (FET) biosensor used for ultrasensitive label-free detection of DNA via peptide nucleic acid (PNA)-DNA hybridization is reported. In this work, R-GO was prepared by reduction of GO with hydrazine, and the FET biosensor was fabricated by drop-casting the R-GO suspension onto the sensor surface. PNA instead of DNA as the capture probe was employed, and DNA detection was performed through PNA-DNA hybridization by the R-GO FET biosensor. The detection limit as low as 100 fM was achieved, which is 1 order of magnitude lower than that of the previously reported graphene FET DNA biosensor based on DNA-DNA hybridization. Moreover, the R-GO FET biosensor was able to distinguish the complementary DNA from one-base mismatched DNA and noncomplementary DNA. Interestingly, the fabricated DNA biosensor was found to have a regeneration capability. The developed R-GO FET DNA biosensor shows ultrasensitivity and high specificity, indicating its potential applications in disease diagnostics as a point-of-care tool.
authors: School of Laboratory Medicine, Hubei University of Chinese Medicine , 1 Huangjia Lake West Road, Wuhan 430065, People's Republic of China.
authors: Bingjie
authors: B
authors: Cai
authors: Shuting
authors: S
authors: Wang
authors: Le
authors: L
authors: Huang
authors: Yong
authors: Y
authors: Ning
authors: Zhiyong
authors: Z
authors: Zhang
authors: Guo-Jun
authors: GJ
authors: Zhang
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation*; D004247:DNA / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D006108:Graphite / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D014173:Transistors, Electronic
chemical_list: D010087:Oxides; D020135:Peptide Nucleic Acids; D006108:Graphite; D004247:DNA