title: Mesenchymal stromal cell injection protects against oxidative stress in Escherichia coli-induced acute lung injury in mice.
abstract: OBJECTIVE
  Stem cells may be a promising therapy for acute respiratory distress syndrome. Recent in vivo and in vitro studies suggested that the mesenchymal stromal cells (MSCs) have anti-oxidative stress properties. We hypothesized that intravenous injection of bone marrow-derived mesenchymal stem cells (MSCs) could attenuate Escherichia coli-induced acute lung injury (ALI) in mice by controlling the oxidative stress status.
  
  
  METHODS
  Eighty mice were randomly divided into four groups: group 1 (control group) received 25 μL of saline as a vehicle; group 2 contained E coli-induced ALI mice; group 3 included mice that received MSCs before induction of ALI; group 4 included mice that received MSCs after induction of ALI. Lung samples were isolated and assayed for oxidative stress variables and histopathologic analysis. Total anti-oxidant capacity was measured in broncho-alveolar lavage.
  
  
  RESULTS
  Pre- and post-injury MSC injection increased survival, reduced pulmonary edema and attenuated lung injuries in ALI mice. Histologically, MSCs exhibited a considerable degree of preservation of the pulmonary alveolar architecture. An increase of anti-oxidant enzyme activities and a decrease of myeloperoxidase activity and malondialdehyde levels in the MSC recipient groups versus the ALI group were found. Furthermore, the total anti-oxidant capacity and reduced glutathione levels were significantly increased in MSCs recipient groups versus the ALI group. Weak +ve inducible nitric oxide synthase immuno-expression in groups that received MSCs was detected. Pre-injury MSC injection showed better effects than did post-injury MSC injection.
  
  
  CONCLUSIONS
  Systemic bone marrow-derived MSC injection was effective in modulating the oxidative stress status in E coli-induced acute lung injury in mice.
authors: Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt. Electronic address: sallyshalaby@hotmail.com.
authors: Sally M
authors: SM
authors: Shalaby
authors: Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors: Amal S
authors: AS
authors: El-Shal
authors: Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors: Somia H
authors: SH
authors: Abd-Allah
authors: Histology and Cell Biology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors: Assmaa O
authors: AO
authors: Selim
authors: Histology and Cell Biology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors: Sally A
authors: SA
authors: Selim
authors: Histology and Cell Biology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors: Zienab A
authors: ZA
authors: Gouda
authors: Clinical Pharmacology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors: Dalia M
authors: DM
authors: Abd El Motteleb
authors: Anesthesia and Intensive Care Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors: Hala E
authors: HE
authors: Zanfaly
authors: Anesthesia and Intensive Care Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors: Heba M
authors: HM
authors: El-Assar
authors: Microbiology and Immunology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors: Shymaa
authors: S
authors: Abdelazim
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000628:therapy*; D000818:Animals; D064987:Cell- and Tissue-Based Therapy; D004926:Escherichia coli / Q000737:chemistry; D007267:Injections; D008070:Lipopolysaccharides / Q000633:toxicity; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D018384:Oxidative Stress
chemical_list: D008070:Lipopolysaccharides
keywords: Escherichia coli; acute lung injury; mesenchymal stromal cells; mice; oxidative stress markers