title: Library generation by gene shuffling.
abstract: This unit describes the process of gene shuffling, also known as sexual PCR. Gene shuffling is a facile method for the generation of sequence libraries containing the information from a family of related genes. Essentially, related genes are fragmented by DNase I digestion and reassembled by primer-less PCR. The resulting chimeric genes can then be screened or selected for a desired function.
authors: University of Texas at Austin, Austin, Texas.
authors: Adam J
authors: AJ
authors: Meyer
authors: Jared W
authors: JW
authors: Ellefson
authors: Andrew D
authors: AD
authors: Ellington
mesh_terms: D017931:DNA Primers / Q000235:genetics*; D003850:Deoxyribonuclease I / Q000378:metabolism; D015723:Gene Library; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; D003850:Deoxyribonuclease I
keywords: PCR; directed evolution; recombination