title: Interleukin-11 induces the expression of matrix metalloproteinase 13 in gastric cancer SCH cells partly via the PI3K-AKT and JAK-STAT3 pathways.
abstract: Interleukin (IL)-11 is expressed in the majority of gastric carcinomas and has been associated with an aggressive phenotype and poor prognosis of gastric adenocarcinoma. Matrix metalloproteinase (MMP)-13 has been detected in numerous invasive malignant tumor types and exhibits a broad spectrum of activities on connective tissue components. In this study, we investigated whether IL-11 affects the expression of MMP-13 in human gastric cancer cells, as well as the underlying mechanism. Using western blot assays, we investigated the effect of recombinant human (rh) IL-11 on the expression of MMP-13 in gastric carcinoma cell lines. Using the PI3K inhibitor wortmannin and RNA interference to target the STAT3 gene, we investigated the effects of PI3K inhibition and/or STAT3 depletion on the expression of the MMP-13 protein. Results showed that IL-11 induced MMP-13 expression in a time- and concentration-dependent manner in SCH cells. IL-11 activated PI3K-AKT and JAK-STAT3 signal transduction. Wortmannin and depletion of STAT3 by means of small interfering RNA (siRNA) synergistically reduced the expression of MMP-13. These findings suggested that IL-11 induces the expression of MMP-13 in gastric cancer SCH cells partly via the PI3K-AKT and JAK-STAT3 pathways.
authors: Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Baiyun, Guangzhou 510515, P.R. China.
authors: Gongli
authors: G
authors: Yang
authors: Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Baiyun, Guangzhou 510515, P.R. China.
authors: Feng
authors: F
authors: Ma
authors: Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Baiyun, Guangzhou 510515, P.R. China.
authors: Muxiao
authors: M
authors: Zhong
authors: Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Baiyun, Guangzhou 510515, P.R. China.
authors: Lin
authors: L
authors: Fang
authors: Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Baiyun, Guangzhou 510515, P.R. China.
authors: Yao
authors: Y
authors: Peng
authors: Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Baiyun, Guangzhou 510515, P.R. China.
authors: Xiaoming
authors: X
authors: Xin
authors: Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Baiyun, Guangzhou 510515, P.R. China.
authors: Jietao
authors: J
authors: Zhong
authors: Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Baiyun, Guangzhou 510515, P.R. China.
authors: Wei
authors: W
authors: Zhu
authors: Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Baiyun, Guangzhou 510515, P.R. China.
authors: Yali
authors: Y
authors: Zhang
mesh_terms: D000730:Androstadienes / Q000494:pharmacology; D045744:Cell Line, Tumor; D020868:Gene Silencing / Q000187:drug effects; D006801:Humans; D017370:Interleukin-11 / Q000494:pharmacology*; D053612:Janus Kinases / Q000378:metabolism*; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D034741:RNA, Small Interfering / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000473:pathology*; D000077191:Wortmannin
chemical_list: D000730:Androstadienes; D017370:Interleukin-11; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; D050796:STAT3 Transcription Factor; D019869:Phosphatidylinositol 3-Kinases; D053612:Janus Kinases; D053509:Matrix Metalloproteinase 13; D000077191:Wortmannin