title: [Comparison of P-nitrobenzoic acid and hydroxylamine hydrochloride for the primary screening of mycobacterial species].
abstract: OBJECTIVE
  To examine the screening methods for mycobacteria recommended by the China Anti-tuberculosis Association, in order to increase laboratory diagnostic accuracy for mycobacterial screening.
  
  
  METHODS
  Using P-nitrobenzoic acid (PNB 0.5 g/L) as the control group, and hydroxylamine hydrochloride (HA, in 125, 150 and 175 mg/L concentrations) as the study group, laboratory preserved strains of H37Rv M.tuberculosis, and standard and clinically isolated strains of M.nontuberculosis (NTM) from Guangzhou Chest Hospital were tested for both PNB and HA sensitivity. Differences between groups were analyzed by χ(2) test.
  
  
  RESULTS
  Among the 2529 MTB strains, the resistance rate to PNB was 3.0% (76/2529), to HA was 12.2% (308/2529), 4.8% (121/2529), and 0.9% (23/2529), respectively, corresponding to the aforementioned 3 different concentrations of HA. Among the 1766 NTM strains, the sensitive rate to PNB was 8.3% (147/1766), to HA was 0.1% (2/1766), 0.5% (9/1766), and 0.9% (16/1766), respectively, corresponding to the aforementioned 3 different concentrations of HA. There was significant difference (χ(2) = 5.44-83.50, P < 0.05).
  
  
  CONCLUSIONS
  HA at 175 mg/L concentration was the optimal condition for laboratory tuberculosis preliminary screening.
authors: Laboratory of Guangzhou Chest Hospital of Laboratory, Guangzhou 510095, China. Email: wlz8160@21cn.com.
authors: Long-zhang
authors: LZ
authors: Wu
authors: Shou-yong
authors: SY
authors: Tan
authors: Yao-ju
authors: YJ
authors: Tan
authors: Yi-luan
authors: YL
authors: Jian
authors: Lu
authors: L
authors: Kuang
authors: Mei-yu
authors: MY
authors: Pan
authors: Shao-fang
authors: SF
authors: Zeng
authors: Yan-wen
authors: YW
authors: Liu
authors: Bing-tang
authors: BT
authors: Zhong
mesh_terms: D003470:Culture Media; D024881:Drug Resistance, Bacterial; D006801:Humans; D019811:Hydroxylamine / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009161:Mycobacterium / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification*; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification; D009579:Nitrobenzoates / Q000494:pharmacology*; D009170:Nontuberculous Mycobacteria / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification
chemical_list: D003470:Culture Media; D009579:Nitrobenzoates; D019811:Hydroxylamine; C008629:4-nitrobenzoic acid