title: Isolation and characterization of BoHV-1 from seropositive cows after inducing artificial stress in West Bengal, India.
abstract: Infectious Bovine Rhinotracheitis (BoHV-1) is the most important emerging disease of cattle in India. With an aim to reactivate BoHV-1 from latently infected sero-positive cattle for molecular characteristics of the isolates prevalent in tropical and sub-tropical countries like India and further epidemiological investigations on IBR infections this study had been conducted. Artificial stress with dexamethasone at the dose rate of 0.1 mg kg(-1) body weight for 5 consecutive days was induced in BoHV-1 sero-positive cows. Then isolation from nasal swabs was attempted in Madin Darby Bovine Kidney (MDBK) cell line to find out the prevalent strain in India. The virus was isolated from all the three cows. All the three isolates were typed as BoHV-1.2 (Strain India 4, India 5 and India 6). The reactivation obtained in this study with dexamethasone suggests the usefulness of BoHV-1 cow latency model for epidemiological investigations on BoHV-1 infections in tropical and sub-tropical countries like India, Pakistan etc.
authors: Department of Veterinary Epidemiology and Preventive Medicine, West Bengal University of Animal and Fishery Sciences, 68 K.B. Sarani, Kolkata-700 037, West Bengal, India.
authors: Tapabrata
authors: T
authors: Saha
authors: Department of Veterinary Epidemiology and Preventive Medicine, West Bengal University of Animal and Fishery Sciences, 68 K.B. Sarani, Kolkata-700 037, West Bengal, India.
authors: Chanchal
authors: C
authors: Guha
authors: Institute of Animal Health and Veterinary Biologicals, Government of West Bengal, 68 K.B. Sarani, Kolkata-700 037, West Bengal, India.
authors: Dhruba
authors: D
authors: Chakraborty
authors: Institute of Animal Health and Veterinary Biologicals, Government of West Bengal, 68 K.B. Sarani, Kolkata-700 037, West Bengal, India.
authors: Biplab
authors: B
authors: Pal
authors: Department of Veterinary Epidemiology and Preventive Medicine, West Bengal University of Animal and Fishery Sciences, 68 K.B. Sarani, Kolkata-700 037, West Bengal, India.
authors: Ujjwal
authors: U
authors: Biswas
authors: Department of Veterinary Epidemiology and Preventive Medicine, West Bengal University of Animal and Fishery Sciences, 68 K.B. Sarani, Kolkata-700 037, West Bengal, India.
authors: Amaresh
authors: A
authors: Chatterjee
authors: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, 17493 Greifswald-InselRiems, Germany.
authors: Patricia
authors: P
authors: Koenig
authors: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, 17493 Greifswald-InselRiems, Germany.
authors: Martin
authors: M
authors: Beer
mesh_terms: D000818:Animals; D002417:Cattle; D003907:Dexamethasone / Q000494:pharmacology; D004285:Dogs; D005838:Genotype; D007242:Herpesvirus 1, Bovine / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000472:pathogenicity; D007194:India; D007241:Infectious Bovine Rhinotracheitis / Q000097:blood / Q000635:transmission / Q000821:virology*; D061985:Madin Darby Canine Kidney Cells; D009296:Nasal Cavity / Q000821:virology; D010641:Phenotype; D013312:Stress, Physiological / Q000187:drug effects; D014775:Virus Activation / Q000187:drug effects; D017735:Virus Latency
chemical_list: D003907:Dexamethasone