title: High-Throughput Single-Cell Analysis for Wound Healing Applications.
abstract: CONCLUSIONS
  Wound repair is a complex biological process that integrates multiple physiologic pathways to restore skin homeostasis after a wide array of gross and anatomical insults. As such, a scientific examination of the wound typically requires broad sampling of numerous factors and is commonly achieved through DNA microarray analysis.
  
  
  RESULTS
  In the last several years, it has become increasingly evident that the granularity afforded by such traditional population-based assays may be insufficient to capture the complex relationships in heterogeneous processes such as those associated with wound healing and stem cell biology.
  
  
  BACKGROUND
  Several emerging technologies have recently become available that permit high-throughput single-cell gene expression analysis in a manner which provides novel insights into the relationships of complex tissue. The most prominent among these employs microfluidic-based devices to achieve a high-resolution analysis of tissue samples.
  
  
  CONCLUSIONS
  The intrinsically heterogeneous nature of injured tissue, in conjunction with its temporal dynamics, makes wound repair and tissue regeneration an attractive target for high-throughput single-cell analysis. Given the staggering costs associated with chronic and non-healing wounds, the development of predictive and diagnostic tools using this technology would likely be attractive to healthcare providers.
authors: Department of Surgery, Stanford University School of Medicine , Stanford, California.
authors: Michael
authors: M
authors: Januszyk
authors: Department of Surgery, Stanford University School of Medicine , Stanford, California.
authors: Geoffrey C
authors: GC
authors: Gurtner