title: Voltage dependent potassium channel remodeling in murine intestinal smooth muscle hypertrophy induced by partial obstruction.
abstract: Partial obstruction of the small intestine causes obvious hypertrophy of smooth muscle cells and motility disorder in the bowel proximate to the obstruction. To identify electric remodeling of hypertrophic smooth muscles in partially obstructed murine small intestine, the patch-clamp and intracellular microelectrode recording methods were used to identify the possible electric remodeling and Western blot, immunofluorescence and immunoprecipitation were utilized to examine the channel protein expression and phosphorylation level changes in this research. After 14 days of obstruction, partial obstruction caused obvious smooth muscle hypertrophy in the proximally located intestine. The slow waves of intestinal smooth muscles in the dilated region were significantly suppressed, their amplitude and frequency were reduced, whilst the resting membrane potentials were depolarized compared with normal and sham animals. The current density of voltage dependent potassium channel (KV) was significantly decreased in the hypertrophic smooth muscle cells and the voltage sensitivity of KV activation was altered. The sensitivity of KV currents (IKV) to TEA, a nonselective potassium channel blocker, increased significantly, but the sensitivity of IKv to 4-AP, a KV blocker, stays the same. The protein levels of KV4.3 and KV2.2 were up-regulated in the hypertrophic smooth muscle cell membrane. The serine and threonine phosphorylation levels of KV4.3 and KV2.2 were significantly increased in the hypertrophic smooth muscle cells. Thus this study represents the first identification of KV channel remodeling in murine small intestinal smooth muscle hypertrophy induced by partial obstruction. The enhanced phosphorylations of KV4.3 and KV2.2 may be involved in this process.
authors: Department of Physiology, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors: Dong-Hai
authors: DH
authors: Liu
authors: Department of Physiology, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors: Xu
authors: X
authors: Huang
authors: Department of Physiology, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors: Xin
authors: X
authors: Guo
authors: Department of Physiology, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors: Xiang-Min
authors: XM
authors: Meng
authors: Department of Physiology, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors: Yi-Song
authors: YS
authors: Wu
authors: Department of Physiology, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors: Hong-Li
authors: HL
authors: Lu
authors: Department of Physiology, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors: Chun-Mei
authors: CM
authors: Zhang
authors: Department of Physiology, Chungbuk National University College of Medicine, Cheongju, Chungbuk, Republic of Korea.
authors: Young-chul
authors: YC
authors: Kim
authors: Department of Physiology, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors: Wen-Xie
authors: WX
authors: Xu
mesh_terms: D015761:4-Aminopyridine / Q000494:pharmacology; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D025802:Electric Capacitance; D005455:Fluorescent Antibody Technique; D005629:Frozen Sections; D006984:Hypertrophy; D007415:Intestinal Obstruction / Q000503:physiopathology*; D015640:Ion Channel Gating / Q000187:drug effects; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D008813:Mice, Inbred ICR; D009130:Muscle, Smooth / Q000187:drug effects / Q000503:physiopathology*; D010766:Phosphorylation / Q000187:drug effects; D021122:Protein Subunits / Q000378:metabolism; D051669:Shab Potassium Channels / Q000378:metabolism*; D051672:Shal Potassium Channels / Q000378:metabolism*; D019789:Tetraethylammonium / Q000494:pharmacology
chemical_list: D021122:Protein Subunits; D051669:Shab Potassium Channels; D051672:Shal Potassium Channels; D019789:Tetraethylammonium; D015761:4-Aminopyridine