title: Generation and use of antibody fragments for structural studies of proteins refractory to crystallization.
abstract: With the rapid technological advances in all aspects of macromolecular X-ray crystallography the preparation of diffraction quality crystals has become the rate-limiting step. Crystallization chaperones have proven effective for overcoming this barrier. Here we describe the usage of a Fab chaperone for the crystallization of HIV-1 Rev, a protein that has long resisted all attempts at elucidating its complete atomic structure.
authors: Protein Expression Laboratory, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, USA.
authors: Stephen J
authors: SJ
authors: Stahl
authors: Norman R
authors: NR
authors: Watts
authors: Paul T
authors: PT
authors: Wingfield
mesh_terms: D001709:Biotechnology / Q000379:methods*; D018360:Crystallography, X-Ray; D015694:Gene Products, rev / Q000378:metabolism*; D015497:HIV-1 / Q000378:metabolism*
chemical_list: D015694:Gene Products, rev