title: Rapid resolution liquid chromatography coupled with quadrupole time-of-flight mass spectrometry-based metabolomics approach to study the effects of jieduquyuziyin prescription on systemic lupus erythematosus.
abstract: Jieduquyuziyin prescription (JP), a traditional Chinese medicine (TCM) prescription, has been widely used for the clinical treatment of systemic lupus erythematosus (SLE). However, the complex chemical constituents of JP and the multifactorial pathogenesis of SLE make research on the therapeutic mechanism of JP in SLE challenging. In this paper, a serum metabolomics approach based on rapid resolution liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (RRLC-Q-TOF/MS) was employed to acquire the metabolic characteristics of serum samples obtained from mice in the SLE model group, JP-treated group, prednisone acetate (PA)-treated group and control group. The orthogonal partial least squares (OPLS) was applied to recognize metabolic patterns, and an obvious separation of groups was obtained. Thirteen metabolites, namely, phosphatidylethanolamine (PE 20:3), hepoxilin B3, lyso- phosphatidylethanolamine (lyso-PE 22:6), 12S-hydroxypentaenoic acid (12S-HEPE), traumatic acid, serotonin, platelet-activating factor (PAF), phosphatidylcholine (PC 20:5),eicosapentaenoic acid (EPA), 12(S)-hydroxyei- cosatetraenoic acid (12S-HETE), 14-hydroxy docosahexaenoic acid (14-HDOHE), lyso-phosphatidylcholine (lyso-PC 20:4), and indole acetaldehyde, were identified and characterized as differential metabolites involved in the pathogenesis of SLE. After treatment with JP, the relative content of 12(S)-HETE, PAF, 12(S)-HEPE, EPA, PE (20:3), Lyso-PE(22:6), and 14-HDOHE were effectively regulated, which suggested that the therapeutic effects of JP on SLE may involve regulating disturbances to the metabolism of unsaturated fatty acid, tryptophan and phospholipid. This research also demonstrated that metabolomics is a powerful tool for researching complex disease mechanisms and evaluating the mechanism of action of TCM.
authors: Analysis and Testing Center, Zhejiang Chinese Medical University, Hangzhou, China.
authors: Xinghong
authors: X
authors: Ding
authors: College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, China.
authors: Jinbo
authors: J
authors: Hu
authors: College of Basic Medicine, Zhejiang Chinese Medical University, Hangzhou, China.
authors: Chengping
authors: C
authors: Wen
authors: College of Life Science, Zhejiang Chinese Medical University, Hangzhou, China.
authors: Zhishan
authors: Z
authors: Ding
authors: College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, China.
authors: Li
authors: L
authors: Yao
authors: College of Basic Medicine, Zhejiang Chinese Medical University, Hangzhou, China.
authors: Yongsheng
authors: Y
authors: Fan
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000378:metabolism / Q000627:therapeutic use*; D005260:Female; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods; D055432:Metabolomics / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D011241:Prednisone / Q000627:therapeutic use
chemical_list: D004365:Drugs, Chinese Herbal; D011241:Prednisone