title: Amino acids modulates the intestinal proteome associated with immune and stress response in weaning pig.
abstract: The objective of this study was to investigate the effects of free amino acids supplementation to protein restricted diet on the intestinal morphology and proteome composition in weaning pigs. Weanling piglets were randomly fed one of the three diets including a corn-soybean based control diet and two lower protein diets with or without free amino acids supplementation for 2 weeks. The jejunum samples of piglets were collected for morphology and proteome analysis. Compared with the control diet, the protein restricted diet had a significant lower average daily gain and higher feed conversion rate. Free amino acids supplementation to the protein restricted diet significantly improved average daily gain and higher feed conversion rate, compared with the protein restricted diet. The villous height in pigs fed the protein restricted diet was lower than that of the control and free amino acids diet. Using two-dimensional gel electrophoresis and mass spectrometry, we identified 16 differentially expressed protein spots in the jejunum of the weaning piglet. These proteins were related to stress and immune response, the metabolism of carbohydrates and lipids, and tissue structure. Based on the proteome and ELISA analysis, free amino acids diet significantly down-regulated the jejunal expression of stress protein heat shock 60 kDa protein. Our results indicated that amino acids supplementation to the protein restricted diet could enhance weight gain and feed efficiency in weanling pigs through improving intestinal nutrient absorption and transportation, gut health, and mucosal immunity.
authors: State Key Laboratory of Animal Nutrition, China Agricultural University, No. 2. Yuanmingyuan West Road, Beijing, 100193, China.
authors: Man
authors: M
authors: Ren
authors: Chuang
authors: C
authors: Liu
authors: Xiangfang
authors: X
authors: Zeng
authors: Longyao
authors: L
authors: Yue
authors: Xiangbing
authors: X
authors: Mao
authors: Shiyan
authors: S
authors: Qiao
authors: Junjun
authors: J
authors: Wang
mesh_terms: D000818:Animals; D004032:Diet; D019587:Dietary Supplements; D007583:Jejunum / Q000378:metabolism; D020543:Proteome / Q000235:genetics*; D013025:Glycine max; D013312:Stress, Physiological / Q000235:genetics* / Q000276:immunology; D034421:Sus scrofa / Q000235:genetics* / Q000378:metabolism; D013552:Swine; D014886:Weaning
chemical_list: D020543:Proteome