title: Mercury arc lamp based super-resolution imaging with conventional fluorescence microscopes.
abstract: We present an implementation of localization based three-dimensional super-resolution imaging on a regular microscope. We retain the original arc lamp as the photoactivation light source, and incorporate an inexpensive diode laser for imaging. As alterations to the standard microscope is minimal, this optical setup can be easily adapted in a typical research laboratory and even undergraduate teaching experiments, providing an inexpensive system for students and research scientists who require such super resolution capabilities. With this simple design, a spatial resolution of better than 40 nm at a reasonable frame rate has been achieved, adequate for most routine applications.
authors: Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China.
authors: Zhiwei
authors: Z
authors: Yuan
authors: Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China.
authors: Jielin
authors: J
authors: Sun
authors: School of Biomedical Engineering and Bio-ID Center, Shanghai Jiao Tong University, Shanghai 200240, China.
authors: Renkuan
authors: R
authors: Zhai
authors: School of Biomedical Engineering and Bio-ID Center, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: xl3a@sjtu.edu.cn.
authors: Xiaowei
authors: X
authors: Li
authors: Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China; School of Biomedical Engineering and Bio-ID Center, Shanghai Jiao Tong University, Shanghai 200240, China; State Key Laboratory for Oncogene and Related Genes, Shanghai Jiao Tong University, Shanghai 200240, China.
authors: Zhifeng
authors: Z
authors: Shao
keywords: Localization-based super-resolution microscopy; Mercury arc lamp; Photoactivation source