title: A continuous cell line, SYSU-OfHe-C, from hemocytes of Ostrinia furnacalis possesses immune ability depending on the presence of larval plasma.
abstract: A continuous cell line, SYSU-OfHe-C, from larval hemocytes of corn borer, Ostrinia furnacalis was established. With increasing passages, the cells grew increasingly faster, and approximately 45% of the cells were in division at passage 55. The culture was mainly composed of two types of cells, granulocytes and plasmatocytes, which showed different division and proliferation behaviors, but possessed similar phagocytic ability. Its spreading ability was significantly weaker than that of hemocytes from naïve larva; however, it could be promoted by larval plasma. Furthermore, its encapsulation ability was also promoted by larval plasma to form multilayer capsules on Sephadex A-25 beads. Finally, the expression of several immune-related genes was verified after provocation by microbes or Sephadex beads. These results indicated that the cell line possessed immune ability depending on the presence of plasma of naïve larvae and are beneficial to studies of insect cellular systems.
authors: State Key Laboratory of Biocontrol, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China. Electronic address: lsshj@mail.sysu.edu.cn.
authors: Jian
authors: J
authors: Hu
authors: State Key Laboratory of Biocontrol, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.
authors: Xiangping
authors: X
authors: Feng
authors: State Key Laboratory of Biocontrol, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.
authors: Zhongguo
authors: Z
authors: Yang
authors: State Key Laboratory of Biocontrol, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.
authors: Zhuoxin
authors: Z
authors: Chen
authors: State Key Laboratory of Biocontrol, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.
authors: Wenqing
authors: W
authors: Zhang
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D048430:Cell Shape; D004926:Escherichia coli / Q000502:physiology; D006098:Granulocytes / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology / Q000648:ultrastructure; D006434:Hemocytes / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology / Q000648:ultrastructure; D006458:Hemolymph / Q000166:cytology; D054884:Host-Pathogen Interactions; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D007814:Larva / Q000166:cytology / Q000276:immunology; D009036:Moths / Q000166:cytology* / Q000276:immunology; D010587:Phagocytosis; D012441:Saccharomyces cerevisiae / Q000502:physiology; D013211:Staphylococcus aureus / Q000502:physiology
chemical_list: D019476:Insect Proteins
keywords: Cell line; Encapsulation; Hemocyte; Immune; Lepidoptera; Spreading