title: Gene Expression and Gene Ontology Enrichment Analysis for H3K4me3 and H3K4me1 in Mouse Liver and Mouse Embryonic Stem Cell Using ChIP-Seq and RNA-Seq.
abstract: Recent study has identified the cis-regulatory elements in the mouse genome as well as their genomic localizations. Recent discoveries have shown the enrichment of H3 lysine 4 trimethylation (H3K4me3) binding as an active promoter and the presence of H3 lysine 4 monomethylation (H3K4me1) outside promoter regions as a mark for an enhancer. In this work, we further identified highly expressed genes by H3K4me3 mark or by both H3K4me3 and H3K4me1 marks in mouse liver using ChIP-Seq and RNA-Seq. We found that in mice, the liver carries embryonic stem cell-related functions while the embryonic stem cell also carries liver-related functions. We also identified novel genes in RNA-Seq experiments for mouse liver and for mouse embryonic stem cells. These genes are not currently in the Ensemble gene database at NCBI.
authors: Department of Computer Science and Engineering, University of Connecticut, Storrs, CT, USA.
authors: Ngoc Tam L
authors: NT
authors: Tran
authors: Department of Computer Science and Engineering, University of Connecticut, Storrs, CT, USA.
authors: Chun-Hsi
authors: CH
authors: Huang
keywords: ChIP-Seq; H3K4me1; H3K4me3; RNA-Seq; gene expression; gene ontology