title: Modification of FP-HIV activity by peptide sequences of GB virus C: a biophysical approach.
abstract: Three synthetic peptide sequences of 18 amino acid each, corresponding to different fragments of the E2 capsid protein of GB virus C (GBV-C): SDRDTVVELSEWGVPCAT (P45), GSVRFPFHRCGAGPKLTK (P58) and RFPFHRCGAGPKLTKDLE (P59) have been characterized in order to find a relationship between their physicochemical properties and the results obtained in cellular models. Experiments were performed in presence and absence of the HIV fusion peptide (FP-HIV) due to the evidences that GBV-C inhibits AIDS progression. P45 peptide showed lower surface activity and less extent of penetration into 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2-dimyristoyl-sn-glycero-3-phospho-L-serine (DMPS) (3:2, mol/mol) lipid monolayers than P58 and P59. However, P45 peptide presented higher capacity to inhibit FP-HIV induced cell-cell fusion than the other two sequences. These results were supported by fluorescence anisotropy measurements which indicated that P45 had a significant effect on the inhibition of FP-HIV perturbation of liposomes of the same lipid composition. Finally, atomic force microscopy (AFM) studies have evidenced the modification of the changes induced by the FP-HIV in the morphology of lipid bilayers when P45 was present in the medium.
authors: Physical Chemistry Department, Faculty of Pharmacy, University of Barcelona, Institute of Nanoscience and Nanotechnology (IN2UB), Avda Joan XXIII, s/n. 08028 Barcelona, Catalonia, Spain.
authors: O
authors: O
authors: Domènech
authors: Physical Chemistry Department, Faculty of Pharmacy, University of Barcelona, Institute of Nanoscience and Nanotechnology (IN2UB), Avda Joan XXIII, s/n. 08028 Barcelona, Catalonia, Spain; Associated Unit to the CSIC Peptides and proteins: Physicochemical properties, Spain.
authors: A
authors: A
authors: Ortiz
authors: Physical Chemistry Department, Faculty of Pharmacy, University of Barcelona, Institute of Nanoscience and Nanotechnology (IN2UB), Avda Joan XXIII, s/n. 08028 Barcelona, Catalonia, Spain; Associated Unit to the CSIC Peptides and proteins: Physicochemical properties, Spain.
authors: M
authors: M
authors: Pujol
authors: Unit of Synthesis and Biomedical Applications of Peptides, Department of Biomedical Chemistry (IQAB-CSIC), Jordi Girona 18-26, 08034 Barcelona, Spain.
authors: I
authors: I
authors: Haro
authors: Physical Chemistry Department, Faculty of Pharmacy, University of Barcelona, Institute of Nanoscience and Nanotechnology (IN2UB), Avda Joan XXIII, s/n. 08028 Barcelona, Catalonia, Spain; Associated Unit to the CSIC Peptides and proteins: Physicochemical properties, Spain.
authors: M
authors: M
authors: Muñoz
authors: Physical Chemistry Department, Faculty of Pharmacy, University of Barcelona, Institute of Nanoscience and Nanotechnology (IN2UB), Avda Joan XXIII, s/n. 08028 Barcelona, Catalonia, Spain; Associated Unit to the CSIC Peptides and proteins: Physicochemical properties, Spain.
authors: M A
authors: MA
authors: Alsina
authors: Physical Chemistry Department, Faculty of Pharmacy, University of Barcelona, Institute of Nanoscience and Nanotechnology (IN2UB), Avda Joan XXIII, s/n. 08028 Barcelona, Catalonia, Spain; Associated Unit to the CSIC Peptides and proteins: Physicochemical properties, Spain.
authors: J
authors: J
authors: Prat
authors: Physical Chemistry Department, Faculty of Pharmacy, University of Barcelona, Institute of Nanoscience and Nanotechnology (IN2UB), Avda Joan XXIII, s/n. 08028 Barcelona, Catalonia, Spain; Associated Unit to the CSIC Peptides and proteins: Physicochemical properties, Spain.
authors: M A
authors: MA
authors: Busquets
authors: Physical Chemistry Department, Faculty of Pharmacy, University of Barcelona, Institute of Nanoscience and Nanotechnology (IN2UB), Avda Joan XXIII, s/n. 08028 Barcelona, Catalonia, Spain; Associated Unit to the CSIC Peptides and proteins: Physicochemical properties, Spain. Electronic address: vgirona@ub.edu.
authors: V
authors: V
authors: Girona
mesh_terms: D000595:Amino Acid Sequence; D001703:Biophysics; D029407:GB virus C / Q000737:chemistry* / Q000378:metabolism; D006678:HIV / Q000737:chemistry* / Q000378:metabolism; D015700:HIV Envelope Protein gp41 / Q000737:chemistry* / Q000378:metabolism; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D015700:HIV Envelope Protein gp41; D008051:Lipid Bilayers; D008081:Liposomes; D008563:Membrane Lipids; D010455:Peptides; D014759:Viral Envelope Proteins; C107619:glycoprotein E2, GB virus C
keywords: Atomic force microscopy; FP-HIV; Fluorescence anisotropy; Synthetic peptides