title: The influence of platelet-rich fibrin on angiogenesis in guided bone regeneration using xenogenic bone substitutes: a study of rabbit cranial defects.
abstract: OBJECTIVE
  The purpose of this study was to investigate the influence of platelet-rich fibrin (PRF) on angiogenesis and osteogenesis in guided bone regeneration (GBR) using xenogenic bone in rabbit cranial defects.
  
  
  METHODS
  In each rabbit, 2 circular bone defects, one on either side of the midline, were prepared using a reamer drill. Each of the experimental sites received bovine bone with PRF, and each of the control sites received bovine bone alone. The animals were sacrificed at 1 week (n = 4), 2 weeks (n = 3) and 4 weeks (n = 3). Biopsy samples were examined histomorphometrically by light microscopy, and expression of vascular endothelial growth factor (VEGF) was determined by immunohistochemical staining.
  
  
  RESULTS
  At all experimental time points, immunostaining intensity for VEGF was consistently higher in the experimental group than in the control group. However, the differences between the control group and the experimental group were not statistically significant in the histomorphometrical and immunohistochemical examinations.
  
  
  CONCLUSIONS
  The results of this study suggest that PRF may increase the number of marrow cells. However, PRF along with xenogenic bone substitutes does not show a significant effect on bony regeneration. Further large-scale studies are needed to confirm our results.
authors: Department of Oral and Maxillofacial Surgery, Yeouido St. Mary's Hospital, Catholic University of Korea, Seoul, Republic of Korea.
authors: Jong-Suk
authors: JS
authors: Yoon
authors: Department of Oral and Maxillofacial Surgery, Yeouido St. Mary's Hospital, Catholic University of Korea, Seoul, Republic of Korea.
authors: Sang-Hwa
authors: SH
authors: Lee
authors: Department of Oral and Maxillofacial Surgery, Yeouido St. Mary's Hospital, Catholic University of Korea, Seoul, Republic of Korea. Electronic address: omfsyhj@catholic.ac.kr.
authors: Hyun-Joong
authors: HJ
authors: Yoon
mesh_terms: D000818:Animals; D001706:Biopsy; D001792:Blood Platelets / Q000502:physiology*; D001847:Bone Diseases / Q000473:pathology / Q000601:surgery*; D001854:Bone Marrow Cells / Q000187:drug effects / Q000473:pathology; D001861:Bone Regeneration / Q000187:drug effects*; D018786:Bone Substitutes / Q000627:therapeutic use*; D016025:Bone Transplantation / Q000379:methods*; D002417:Cattle; D005337:Fibrin / Q000627:therapeutic use*; D048091:Guided Tissue Regeneration / Q000379:methods*; D064593:Heterografts / Q000473:pathology / Q000637:transplantation*; D008297:Male; D008903:Minerals / Q000627:therapeutic use; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects; D010294:Parietal Bone / Q000473:pathology / Q000601:surgery*; D011817:Rabbits; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis
chemical_list: C077540:Bio-Oss; D018786:Bone Substitutes; D008903:Minerals; D042461:Vascular Endothelial Growth Factor A; D005337:Fibrin
keywords: Non-organic bovine bone; PRF (platelet-rich fibrin); Vascular endothelial growth factor