title: Genetic profiling of Klebsiella pneumoniae: comparison of pulsed field gel electrophoresis and random amplified polymorphic DNA.
abstract: In this study, the discriminatory power of pulsed field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD) methods for subtyping of 54 clinical isolates of Klebsiella pneumoniae were compared. All isolates were typeable by RAPD, while 3.6% of them were not typeable by PFGE. The repeatability of both typing methods were 100% with satisfying reproducibility (≥ 95%). Although the discriminatory power of PFGE was greater than RAPD, both methods showed sufficient discriminatory power (DI > 0.95) which reflects the heterogeneity among the K. pneumoniae isolates. An optimized RAPD protocol is less technically demanding and time consuming that makes it a reliable typing method and competitive with PFGE.
authors: Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, G.C., Tehran, Iran.
authors: Mitra
authors: M
authors: Ashayeri-Panah
authors: Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, G.C., Tehran, Iran.
authors: Fereshteh
authors: F
authors: Eftekhar
authors: Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, G.C., Tehran, Iran.
authors: Maryam Mobarak
authors: MM
authors: Ghamsari
authors: Infectious Disease and Tropical Disease Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors: Mahmood
authors: M
authors: Parvin
authors: Department of Microbiology, School of Medicine, Tehran University of Medical Sciences and Pediatric Infectious Disease Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors: Mohammad Mehdi
authors: MM
authors: Feizabadi
mesh_terms: D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007711:Klebsiella pneumoniae / Q000145:classification* / Q000235:genetics*; D008297:Male; D058889:Molecular Typing / Q000379:methods*; D019105:Random Amplified Polymorphic DNA Technique; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
keywords: Klebsiella pneumoniae; genotyping; pulsed field gel electrophoresis; random amplified polymorphic DNA