title: Different subsets of macrophages in patients with new onset tuberculous pleural effusion.
abstract: OBJECTIVE
  Macrophages are the infiltrate components of tuberculous pleural effusion (TPE). This study is aimed at examining the role of different subsets of macrophages in pleural fluid (PF) and peripheral blood (PB) from patients with new onset TPE.
  
  
  METHODS
  The numbers of PB and PF CD163(+), CD206(+) and CD115(+) macrophages in 25 patients with new onset TPE and 17 healthy controls (HC) were determined by flow cytometry. The concentrations of serum and PF cytokines were determined by cytometric bead array (CBA) and enzyme-linked immunosorbentassay (ELISA). The potential association between the numbers of different subsets of macrophages and the values of clinical measures in TPE patients were analyzed.
  
  
  RESULTS
  The numbers of PB CD14(+)CD163(-) M1-like and CD14(+)CD163(-) interleukin (IL)-12(+) M1 macrophages were significantly higher than that in the HC, but lower than PF, and the numbers of PF CD14(+)CD163(+), CD14(+)CD163(+)CD206(+), CD14(+)CD163(+)CDll5(+) M2-like, and CD14(+)CD163(+)IL-10(+) M2 macrophages were less than PB in the TPE patients. The levels of serum IL-1, IL-6, IL-8, IL-12, tumor growth factor (TGF)-β1, and tumor necrosis factor (TNF)-α in the TPE patients were significantly higher than that in the HC, but lower than that in the PF. The levels of PF IL-10 were significantly higher than that in the PB of patients and HC. In addition, the levels of serum IL-12 and TNF-α were correlated positively with the values of erythrocyte sedimentation rate (ESR) and the numbers of ESAT-6- and culture filtrate protein 10 (CFP-10)-specific IFN-γ-secreting T cells, and the levels of PF TNF-α were correlated positively with the levels of PF adenosine deaminase (ADA) and lactate dehydrogenase (LDH) in those patients.
  
  
  CONCLUSIONS
  Our data indicate that Mycobacterium tuberculosis (M. tb) infection induces M1 predominant pro-inflammatory responses, contributing to the development of TPE in humans.
authors: Department of Respiratory Medicine, The First Hospital, Jilin University, Changchun, China.
authors: Ying
authors: Y
authors: Tang
authors: Department of Respiratory Medicine, The First Hospital, Jilin University, Changchun, China.
authors: Shu-Cheng
authors: SC
authors: Hua
authors: The Center of Tuberculous Meningitis Diagnosis and Treatment, The Infectious Disease Hospital of Changchun, Changchun, China.
authors: Gui-Xiang
authors: GX
authors: Qin
authors: Department of Respiratory Medicine, The First Hospital, Jilin University, Changchun, China.
authors: Li-Jun
authors: LJ
authors: Xu
authors: Key Laboratory for Zoonosis Research, Ministry of Education; Department of Central Laboratory, The First Hospital, Jilin University, Changchun, China.
authors: Yan-Fang
authors: YF
authors: Jiang
mesh_terms: D000328:Adult; D000368:Aged; D002452:Cell Count; D003710:Demography; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016753:Interleukin-10 / Q000097:blood; D018664:Interleukin-12 / Q000097:blood; D037181:Lectins, C-Type / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism / Q000473:pathology*; D008297:Male; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000378:metabolism; D008875:Middle Aged; D010996:Pleural Effusion / Q000097:blood / Q000473:pathology*; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D014396:Tuberculosis, Pleural / Q000097:blood / Q000473:pathology*; D055815:Young Adult
chemical_list: D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface; D016753:Interleukin-10; D018664:Interleukin-12; D016186:Receptor, Macrophage Colony-Stimulating Factor