title: Therapeutic effect of the treatment for colorectal cancer with adenoviral vectors mediated estrogen receptor β gene therapy combined with thermotherapy.
abstract: BACKGROUND
  Our preliminary study on repressing colorectal tumors by recombinant adenoviruses (Ads) delivering the human ERβ gene (Ad-ERβ) has achieved positive result.
  
  
  METHODS
  In this study, hydrophobic fluorescent dyes ICG-Der-01 was entrapped into the N-succinyl-N'-octyl chitosan (SOC) micelles to form the near infrared absorbing dyes SOC-ICG-Der-01 and SOC-ICG-Der-01 mediated near infrared laser (SOC-ICG-Der-01/NIR) thermotherapy was combined with Ad-ERβ gene therapy to regress colon cancer in vivo.
  
  
  RESULTS
  Firstly, the antitumor efficacies of SOC-ICG-Der-01/NIR thermotherapy were investigated on S180 ascites tumor-bearing mice. Results indicated that, the average tumor volume of SOC-ICG-Der-01/NIR group was the smallest among the three treatment groups. Then, thermotherapy with SOC-ICG-Der-01/NIR combined with Ad-ERβ gene therapy to treat HCT-116 colon cancer xenograft model was investigated. Further results demonstrated that, SOC-ICG-Der-01/NIR thermotherapy showed the significantly inhibitory efficiency compared with control group and Ad-ERβ enhanced the therapeutic effect of SOC-ICG-Der-01/NIR.
  
  
  CONCLUSIONS
  These findings demonstrated that combined administration of Ad-ERβ with SOC-ICG-Der-01/NIR thermotherapy represents a promising colon cancer therapeutic strategy.
authors: Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, Nanjing, 210023,, China.
authors: Zhenzhen
authors: Z
authors: Tu
authors: Yuxiang
authors: Y
authors: Ma
authors: Walter
authors: W
authors: Akers
authors: Samuel
authors: S
authors: Achilefu
authors: Yueqing
authors: Y
authors: Gu
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000628:therapy*; D003131:Combined Modality Therapy; D047629:Estrogen Receptor beta / Q000235:genetics*; D005456:Fluorescent Dyes; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000008:administration & dosage; D006801:Humans; D006979:Hyperthermia, Induced; D053685:Laser Therapy; D051379:Mice; D008819:Mice, Nude; D012510:Sarcoma 180 / Q000235:genetics / Q000628:therapy*; D014407:Tumor Cells, Cultured
chemical_list: D047629:Estrogen Receptor beta; D005456:Fluorescent Dyes