title: Effect of verapamil and lidocaine on TRPM and NaV1.9 gene expressions in renal ischemia-reperfusion.
abstract: BACKGROUND
  To determine effects on calcium and sodium channels of Ca(2+) and Na(+) channel blockers in the present study, expression levels of TRPM1, TRPM2, TRPM3, TRPM4, TRPM5, TRPM6, TRPM7, TRPM8, and NaV1.9 genes were evaluated in kidney tissues after induced ischemia-reperfusion.
  
  
  METHODS
  Forty albino Wistar male rats were equally divided into 4 groups as follows: group I: control group (n = 10), group II: ischemia group (60 minutes of ischemia + 48 hours of reperfusion; n = 10), group III: ischemia (60 minutes of ischemia + 48 hours of reperfusion) + calcium channel blocker (n = 8), group IV: ischemia (60 minutes of ischemia + 48 hours of reperfusion) + sodium channel blocker (n = 8).
  
  
  RESULTS
  When compared to ischemia group expression levels of TRPM2, TRPM4, TRPM6, and NaV1.9 in Ca(2+) and Na(+) channel blocker groups were increased, whereas that of TRPM7 was decreased. However, expression levels of TRPM1, TRPM3, TRPM5, and TRPM8 were not determined in kidney tissue. Histologically, the Ca(2+) channel blocker verapamil and the Na(+) channel blocker lidocaine inhibited the cell death in kidney tissue compared to control.
  
  
  CONCLUSIONS
  Our study suggested that verapamil and lidocaine significantly reduce the degree of ischemia-reperfusion injury due to effects to TRPM and Nav1.9 genes.
authors: Department of Medical Physiology, Faculty of Medicine, University of Gaziantep, Turkey.
authors: D
authors: D
authors: Dusmez
authors: Department of Medical Physiology, Faculty of Medicine, University of Gaziantep, Turkey. Electronic address: beyhancengiz@gmail.com.
authors: B
authors: B
authors: Cengiz
authors: Department of Medical Biology, Faculty of Medicine, University of Adiyaman, Turkey.
authors: O
authors: O
authors: Yumrutas
authors: Department of Medical Physiology, Faculty of Medicine, University of Gaziantep, Turkey.
authors: T
authors: T
authors: Demir
authors: Department of Medical Biology, Faculty of Medicine, University of Gaziantep, Turkey.
authors: S
authors: S
authors: Oztuzcu
authors: Department of Medical Physiology, Faculty of Medicine, University of Gaziantep, Turkey.
authors: S
authors: S
authors: Demiryurek
authors: Department of Pathology, Faculty of Medicine, University of Gaziantep, Turkey.
authors: E
authors: E
authors: Tutar
authors: Department of Medical Physiology, Faculty of Medicine, University of Gaziantep, Turkey; Department of Medical Biology, Faculty of Medicine, University of Gaziantep, Turkey.
authors: R
authors: R
authors: Bayraktar
authors: Department of Medical Physiology, Faculty of Medicine, University of Adiyaman, Turkey.
authors: A
authors: A
authors: Bulut
authors: Department of Medical Physiology, Faculty of Medicine, University of Dumlupınar, Kutahya, Turkey.
authors: H
authors: H
authors: Simsek
authors: Department of Medical Physiology, Faculty of Medicine, University of Harran, Sanliufa, Turkey.
authors: S Nur
authors: SN
authors: Daglı
authors: Department of Medical Physiology, Faculty of Medicine, University of Gaziantep, Turkey.
authors: T
authors: T
authors: Kılıc
authors: Department of Medical Physiology, Faculty of Medicine, University of Gaziantep, Turkey.
authors: C
authors: C
authors: Bagcı
mesh_terms: D000818:Animals; D002121:Calcium Channel Blockers / Q000008:administration & dosage*; D015220:Calcium Channels / Q000378:metabolism; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D007668:Kidney / Q000473:pathology*; D007674:Kidney Diseases / Q000188:drug therapy* / Q000473:pathology*; D008012:Lidocaine / Q000008:administration & dosage*; D008297:Male; D062558:NAV1.9 Voltage-Gated Sodium Channel / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy*; D050053:TRPM Cation Channels / Q000378:metabolism*; D014700:Verapamil / Q000008:administration & dosage*; D061567:Voltage-Gated Sodium Channel Blockers / Q000008:administration & dosage*
chemical_list: D002121:Calcium Channel Blockers; D015220:Calcium Channels; D062558:NAV1.9 Voltage-Gated Sodium Channel; C113705:Scn11a protein, rat; D050053:TRPM Cation Channels; D061567:Voltage-Gated Sodium Channel Blockers; D008012:Lidocaine; D014700:Verapamil