title: Vaccination with profilin encapsulated in oligomannose-coated liposomes induces significant protective immunity against Toxoplasma gondii.
abstract: Toxoplasma gondii is an obligate intracellular parasite that can infect a variety of mammals and birds, causing toxoplasmosis. Several types of vaccines against T. gondii have been developed, but these have limitations in terms of their safety and inadequate efficacy. T. gondii profilin (TgPF) is a potential immunodominant antigen for a candidate vaccine. In this study, we encapsulated TgPF in oligomannose-coated liposomes (OMLs) to evaluate the immune response induced by this vaccine. C57BL/6 mice were immunized with TgPF-OML three times at 14-day intervals and challenged with T. gondii. TgPF-OML increased the survival of the mice and reduced the parasite burden in their brains after T. gondii infection. Immunization with TgPF-OML also induced TgPF-specific interferon-γ production and IgG antibodies in mice. Our results demonstrate that OML-encapsulated TgPF triggers strong humoral and cellular responses against T. gondii, and that TgPF-OML is a candidate vaccine that warrants further development.
authors: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro 080-8555, Hokkaido, Japan; Faculty of Agriculture, Shinshu University, Minami-Minowa, Kamiina, Nagano 399-4598, Japan.
authors: Sachi
authors: S
authors: Tanaka
authors: Department of Applied Biochemistry, Tokai University, Kita-kaname, Hiratsuka 259-1292, Kanagawa, Japan.
authors: Yasuhiro
authors: Y
authors: Kuroda
authors: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro 080-8555, Hokkaido, Japan.
authors: Fumiaki
authors: F
authors: Ihara
authors: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro 080-8555, Hokkaido, Japan.
authors: Maki
authors: M
authors: Nishimura
authors: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro 080-8555, Hokkaido, Japan.
authors: Jun
authors: J
authors: Hiasa
authors: Department of Applied Biochemistry, Tokai University, Kita-kaname, Hiratsuka 259-1292, Kanagawa, Japan.
authors: Naoya
authors: N
authors: Kojima
authors: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro 080-8555, Hokkaido, Japan. Electronic address: nisikawa@obihiro.ac.jp.
authors: Yoshifumi
authors: Y
authors: Nishikawa
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001921:Brain / Q000469:parasitology; D005260:Female; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D016056:Immunodominant Epitopes / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D008081:Liposomes / Q000276:immunology*; D008358:Mannose / Q000737:chemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D051304:Profilins / Q000276:immunology*; D015800:Protozoan Proteins / Q000276:immunology; D016052:Protozoan Vaccines / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014122:Toxoplasma; D014124:Toxoplasmosis, Animal / Q000517:prevention & control*
chemical_list: D000913:Antibodies, Protozoan; D016056:Immunodominant Epitopes; D008081:Liposomes; D051304:Profilins; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D011994:Recombinant Proteins; D007371:Interferon-gamma; D008358:Mannose
keywords: Oligomannose-coated liposome; Profilin; Toxoplasma gondii; Vaccine