title: On the GFP-based analysis of dynamic concentration profiles.
abstract: Studies with GFP-tagged proteins can be used to investigate the dynamics of concentration profiles of regulatory proteins in cells and tissues. Analysis of these experiments must account for the finite rate with which the GFP-tagged proteins mature to the fluorescent state. Toward this end, we present an analytical framework that provides an explicit connection between the apparent kinetics of concentration gradients and the rates of GFP maturation.
authors: Mathematical and Statistical Computing Laboratory, Division of Computational Bioscience, Center for Information Technology, National Institutes of Health, Bethesda, Maryland.
authors: Alexander M
authors: AM
authors: Berezhkovskii
authors: Department of Chemical and Biological Engineering and Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey. Electronic address: stas@princeton.edu.
authors: Stanislav Y
authors: SY
authors: Shvartsman
mesh_terms: D000818:Animals; D004330:Drosophila / Q000196:embryology / Q000235:genetics / Q000378:metabolism*; D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008954:Models, Biological; D009024:Morphogenesis
chemical_list: D049452:Green Fluorescent Proteins