title: [Simultaneous determination of adrenaline, noradrenaline, cortisone and cortisol in plasma with HPLC/MS/MS].
abstract: OBJECTIVE
  To develop a method for simultaneous determination of adrenaline, noradrenaline, cortisone and cortisol in plasma using HPLC/MS/MS.
  
  
  METHODS
  Sample proteins were precipitated with acetonitrile and the sample solution was injected into HPLC/MS/MS after centrifugation at 15,000 r/min for 5 min. Electrospray ionization (ESI) and the positive ion detection were applied with a multiple reaction monitoring (MRM) mode for quantitative analyses.
  
  
  RESULTS
  Under the optimal conditions, good linearity (r > 0.999) was observed in the range of 0.02-200.00 ng/mL of target compounds. The detection limit reached 4.13 pg/mL, 4.64 pg/mL, 4.29 pg/mL and 4.52 pg/mL for adrenaline, noradrenaline, cortisone and cortisol respectively. The inter-day and intra-day precisions ranged from 1.19%-5.42% and 2.16%-6.04% respectively. Satisfied results were achieved using human plasma samples, with a spiked recovery in the range of 80.0%-109.0% and a relative standard deviation of 3.93%-7.57%.
  
  
  CONCLUSIONS
  The proposed method is quick, sensitive and suitable for batch analyses of plasma samples.
authors: Center for Disease Control and Prevention of Sichuan Province, Chengdu 610041, China.
authors: Li
authors: L
authors: Yong
authors: Department of Sanitary Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
authors: Yu
authors: Y
authors: Wang
authors: Department of Sanitary Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
authors: Xiao-Li
authors: XL
authors: Zou
authors: Center for Disease Control and Prevention of Sichuan Province, Chengdu 610041, China.
authors: Lan
authors: L
authors: Zhu
authors: State Key Laboratory of Oral Disease Research, Sichuan University, Chengdu 610041, China.
authors: Hui-Ru
authors: HR
authors: Xie
authors: State Key Laboratory of Oral Disease Research, Sichuan University, Chengdu 610041, China.
authors: Long-Jiang
authors: LJ
authors: Li
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003348:Cortisone / Q000097:blood*; D004837:Epinephrine / Q000097:blood*; D006801:Humans; D006854:Hydrocortisone / Q000097:blood*; D057230:Limit of Detection; D009638:Norepinephrine / Q000097:blood*; D053719:Tandem Mass Spectrometry
chemical_list: D003348:Cortisone; D006854:Hydrocortisone; D009638:Norepinephrine; D004837:Epinephrine