title: Hypoxic Culturing Enhances the Wound-Healing Potential of Adipose-Derived Stem Cells.
abstract: BACKGROUND
  Adipose tissue is one of the richest sources of mesenchymal stem cells that exhibit an outstanding ability to regenerate skin.
  
  
  OBJECTIVE
  Although the anatomical sites of adipose-derived stem cells (ASCs) in the body are relatively oxygen-deficient (i.e., 1%-5% oxygen content), ASCs are usually cultured under normoxic conditions, and long-term culturing of ASCs in normoxia may induce their senescence.
  The review is an overview of the cellular responses of ASCs during hypoxia, which collectively increase the wound-healing potential of ASCs. Furthermore, the mechanism of action for stimulation by hypoxia (i.e., a pivotal role of reactive oxygen species and related signal pathways) will be discussed.
  Hypoxia is a critical stimulatory factor for ASCs. Therefore, understanding the response and adaptation of ASCs to hypoxia may be invaluable for developing novel cell therapeutic strategies.
  
  
  CONCLUSIONS
  Culturing ASCs under hypoxia may uniquely benefit proliferation, stemness, migration, and growth factor secretion. Therefore, the preconditioning of ASCs by hypoxia shows a prominent wound-healing effect in clinical use.
authors: Department of Dermatology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine , Seoul, Korea.
authors: Won-Serk
authors: WS
authors: Kim
authors: Laboratory of Skin Regeneration, Department of Applied Bioscience, CHA University , Seoul, Korea. ; Stem Cell Research Laboratory, CHA Stem Cell Institute , Seoul, Korea.
authors: Jong-Hyuk
authors: JH
authors: Sung