title: Mass transfer mechanism in chiral reversed phase liquid chromatography.
abstract: The mechanism of mass transfer in chiral chromatography was investigated using an experimental protocol already applied in RPLC and HILIC chromatography. The different contributions to the reduced height equivalent to a theoretical plate (HETP) include the longitudinal diffusion HETP term, the solid-liquid mass transfer resistance HETP term, the short-range eddy dispersion HETP term, and the long-range eddy dispersion HETP term. Their accurate measurement permits the determination of the adsorption rate constant kads of trans-stilbene enantiomers on a column packed with Lux 5 μm Cellulose-1 particles. The experimental results demonstrate that the number of adsorption-desorption steps per unit time of chiral compounds on polysaccharide-based chiral stationary phases is four orders of magnitude smaller than that of achiral compounds.
authors: Department of Chemistry, University of Tennessee, Knoxville, TN 37996-1600, USA.
authors: Fabrice
authors: F
authors: Gritti
authors: Department of Chemistry, University of Tennessee, Knoxville, TN 37996-1600, USA. Electronic address: guiochon@utk.edu.
authors: Georges
authors: G
authors: Guiochon
mesh_terms: D000327:Adsorption; D002853:Chromatography, Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D004058:Diffusion; D008970:Molecular Weight; D013237:Stereoisomerism
keywords: Adsorption–desorption steps; Chiral separation; Lux 5μm Cellulose-1; Mass transfer resistance; Tri-tert-butylbenzene; trans-Stilbene