title: Structural elucidation and antioxidant activities of exopolysaccharides from Lactobacillus helveticus MB2-1.
abstract: In the present study, the crude polysaccharides (EPS) from Lactobacillus helveticus MB2-1 were fermented with reconstituted whey medium and further fractionated on DEAE-52 cellulose and Sephadex G-100 chromatography to afford three purified fractions of EPS-1, EPS-2 and EPS-3. Then, their preliminary structures and antioxidant activities in vitro were investigated. Three purified EPS fractions (EPS-1, EPS-2 and EPS-3) had similar molecular weight distributions (about 2 × 10(5)Da), and it was composed of galactose, glucose and mannose with a molar ratio of 1.33:2.75:1.00, 1.00:1.43:9.34 and 1.17:1.00:2.96, respectively. In addition, the antioxidant activity of EPS-1, EPS-2 and EPS-3 was evaluated with the in vitro scavenging abilities. The results indicated that both crude and purified EPS showed strong scavenging activities on three kinds of radical and chelating activities on ferrous ion, and their antioxidant activities decreased in the order of crude EPS>EPS-3>EPS-2>EPS-1.
authors: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors: Wei
authors: W
authors: Li
authors: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors: Juan
authors: J
authors: Ji
authors: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors: Xiaohong
authors: X
authors: Chen
authors: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors: Mei
authors: M
authors: Jiang
authors: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors: Xin
authors: X
authors: Rui
authors: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. Electronic address: dongms@njau.edu.cn.
authors: Mingsheng
authors: M
authors: Dong
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D002236:Carbohydrate Conformation; D002853:Chromatography, Liquid; D052198:Lactobacillus helveticus / Q000737:chemistry* / Q000254:growth & development; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000975:Antioxidants; D011134:Polysaccharides
keywords: Antioxidant activities; Exopolysaccharides (EPS); Lactobacillus helveticus MB2-1; Purification; Structural elucidation