title: Changes in Rubisco activase gene expression and polypeptide content in Brachypodium distachyon.
abstract: Regulation of Rubisco (D-ribulose-1,5-bisphosphate carboxylase/oxygenase activase (RCA) gene expression and polypeptide content were determined in Brachypodium distachyon leaves, stems and ear elements at different developmental stages under optimal growth conditions as well as under drought and salt stress conditions. B. distachyon leaf contains a much greater amount of Rubisco activase small (RCAS) isoform than the large one (RCAL) under optimal growth conditions. Increased levels of the RCAL isoform compared with the RCAS isoform were found in leaves and in green stems under salt and drought stress, respectively. Transcriptional levels of RCA are almost identical in different leaf positions. Short-term drought and salt stresses did not cause the impairment of RCA gene expression in early seedlings. But gradually increasing drought stress significantly decreased gene expression in early seedling samples. Amounts of the RCAS isoform were found to be more in different leaves of the plant compared with the RCAL isoform and their ratio was constant under normal condition. In green stems gene expression of RCA decreased under salt and drought stresses, although as it was in green leaves protein amounts of RCAL isoform increased compared with the RCAS isoform. All of the above described results clearly indicate that the accumulation of each RCA isoform is differentially regulated by developmental and environmental cues.
authors: Institute of Botany, Azerbaijan National Academy of Sciences, 40 Patamdar Shosse, AZ-1073 Baku, Azerbaijan. Electronic address: sbayramov@hotmail.com.
authors: Shahniyar
authors: S
authors: Bayramov
authors: Institute of Botany, Azerbaijan National Academy of Sciences, 40 Patamdar Shosse, AZ-1073 Baku, Azerbaijan.
authors: Novruz
authors: N
authors: Guliyev
mesh_terms: D058431:Brachypodium / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D055864:Droughts; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D010788:Photosynthesis; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018547:Plant Stems / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D018749:RNA, Plant / Q000235:genetics; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics / Q000378:metabolism; D012274:Ribulosephosphates / Q000378:metabolism; D054712:Salinity; D036226:Seedlings / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D013312:Stress, Physiological
chemical_list: D010940:Plant Proteins; D018749:RNA, Plant; D012274:Ribulosephosphates; C054765:rca protein, plant; C001933:ribulose-1,5 diphosphate; D012273:Ribulose-Bisphosphate Carboxylase
keywords: Brachypodium distachyon; Drought stress; Gene expression; Protein content; Rubisco activase; Salt stress