title: In-situ detection of resveratrol inhibition effect on epidermal growth factor receptor of living MCF-7 cells by Atomic Force Microscopy.
abstract: A new method based on Atomic Force Microscopy (AFM) was developed to real-time and in-situ detect epidermal growth factor receptor (EGFR) expression levels on living MCF-7 cells for evaluating the anticancer activity of resveratrol. Here, the inhibition effect of resveratrol on EGFR expression levels on MCF-7 cells was probed by epidermal growth factor (EGF)-functionalized tips for the first time. Changes in morphology and stiffness of single cell stimulated by resveratrol at different concentrations were detected by AFM. The consequences showed that resveratrol influenced the cellular state and reduced expression of EGFR on the cell surface, which were also interpreted by MTT assay and confocal microscopy assay. AFM, which was used to investigate potential targets for anti-tumor drug on living cells and realize a better understanding of drug action mechanism, was expected to be developed into a promising tool for screening of drugs.
authors: Department of Chemistry, Jinan University, Guangzhou 510632, PR China.
authors: Lu
authors: L
authors: Zhang
authors: Department of Chemistry, Jinan University, Guangzhou 510632, PR China.
authors: Fen
authors: F
authors: Yang
authors: Department of Chemistry, Jinan University, Guangzhou 510632, PR China.
authors: Ji-Ye
authors: JY
authors: Cai
authors: Department of Chemistry, Jinan University, Guangzhou 510632, PR China. Electronic address: typh@jnu.edu.cn.
authors: Pei-Hui
authors: PH
authors: Yang
authors: Experiment and Technology Center, Jinan University, Guangzhou 510632, PR China. Electronic address: zhihong3615@sina.com.
authors: Zhi-Hong
authors: ZH
authors: Liang
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000378:metabolism; D066246:ErbB Receptors / Q000032:analysis* / Q000037:antagonists & inhibitors* / Q000378:metabolism; D005260:Female; D006801:Humans; D055767:Immobilized Proteins / Q000378:metabolism; D061986:MCF-7 Cells; D018625:Microscopy, Atomic Force / Q000379:methods; D061848:Optical Imaging; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D055767:Immobilized Proteins; D013267:Stilbenes; D004815:Epidermal Growth Factor; D066246:ErbB Receptors; D000077185:Resveratrol
keywords: Atomic Force Microscopy; Epidermal growth factor receptor; Force curves; Living MCF-7 cells; Resveratrol