title: Differential expression pattern of microRNAs in CD4+ and CD19+ cells from asymptomatic patients with systemic lupus erythematosus.
abstract: OBJECTIVE
  The aim of this study was to investigate the pattern of microRNA (miRNA) expression in CD19+ and CD4+ cells from asymptomatic patients with systemic lupus erythematosus (SLE).
  
  
  METHODS
  A screening of the expression of 377 miRNAs was performed in human CD4+ and CD19+ cells isolated from the peripheral blood by using a TaqMan Human MicroRNA Array. Validation of differential expression pattern of those was performed using TaqMan assays in these cell populations obtained from a larger cohort of patients and controls.
  
  
  RESULTS
  According to the screening assays, three miRNAs were differentially expressed (p value <0.1) in cell populations from both patients and controls: hsa-miR-143, hsa-miR-224 and hsa-miR-576-5p for CD4+ cells, and hsa-miR-10a, hsa-miR-31 and hsa-miR-345 for CD19+ cells. After validation, significant differences (p value <0.05) were confirmed only for hsa-miR-143 and hsa-miR-224 in CD4+ cells and for hsa-miR-10a and hsa-miR-345 in CD19+ cells. In all cases, the miRNAs were over expressed in SLE patients compared with healthy donors.
  
  
  CONCLUSIONS
  Our results support a different pattern of miRNA expression in SLE patients.
authors: 1Servicio de Inmunología, Hospital Universitario Virgen del Rocío/Instituto de Biomedicina de Sevilla (IBIS), Seville, Spain.
authors: R
authors: R
authors: Martínez-Ramos
authors: J-R
authors: JR
authors: García-Lozano
authors: J-M
authors: JM
authors: Lucena
authors: M-J
authors: MJ
authors: Castillo-Palma
authors: F
authors: F
authors: García-Hernández
authors: M-C
authors: MC
authors: Rodríguez
authors: A
authors: A
authors: Núñez-Roldán
authors: M-F
authors: MF
authors: González-Escribano
mesh_terms: D000328:Adult; D018941:Antigens, CD19 / Q000378:metabolism*; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods
chemical_list: D018941:Antigens, CD19; D035683:MicroRNAs
keywords: Systemic lupus erythematosus (SLE); asymptomatic phase; microRNA