title: Age estimation via quantification of signal-joint T cell receptor excision circles in Koreans.
abstract: The estimation of age from biological samples (i.e., remains) at crime scenes could provide useful information about both victims and other persons related to criminal activities. Signal-joint T cell receptor excision circle (sjTREC) levels in peripheral blood decline with age, and negative correlations between sjTREC levels and age have been demonstrated in several ethnic groups. To validate the utility of sjTREC for age estimation in Koreans, Taqman qPCR was used to quantify the sjTREC level in samples obtained from 172 individuals ranging from 16 to 65 years old. We modified the previously reported method by using a shorter amplicon and confirmed the efficiency and utility of this method in this report. Our results showed that the linear negative regression curve between sjTREC levels and age was characterized by r=-0.807 and a standard error of 8.49 years. These results indicate that sjTREC level is an effective age estimation method in Koreans. The value of the standard error of quantification was not different from previous reports for other population groups.
authors: Department of Forensic Medicine, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, Seoul 110-799, South Korea.
authors: Sohee
authors: S
authors: Cho
authors: Institute of Applied Genetics, Department of Forensics and Investigative Genetics, University of North Texas Health Science Center, 3500 Camp Bowie Blvd., Fort Worth, TX 76107, United States.
authors: Jianye
authors: J
authors: Ge
authors: Institute of Applied Genetics, Department of Forensics and Investigative Genetics, University of North Texas Health Science Center, 3500 Camp Bowie Blvd., Fort Worth, TX 76107, United States.
authors: Seung Bum
authors: SB
authors: Seo
authors: Department of Forensic Medicine, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, Seoul 110-799, South Korea.
authors: Kiha
authors: K
authors: Kim
authors: Department of Forensic Medicine, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, Seoul 110-799, South Korea.
authors: Hye Young
authors: HY
authors: Lee
authors: Department of Forensic Medicine, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, Seoul 110-799, South Korea; Institute of Forensic Medicine, Seoul National University College of Medicine, 28, Yongon-Dong, Chongno-Gu, Seoul 110-799, South Korea. Electronic address: sdlee@snu.ac.kr.
authors: Soong Deok
authors: SD
authors: Lee
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging / Q000208:ethnology* / Q000235:genetics / Q000502:physiology; D044466:Asian People / Q000235:genetics; D053803:Forensic Genetics / Q000379:methods; D049429:Forensic Pathology; D015870:Gene Expression; D015321:Gene Rearrangement; D006801:Humans; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D011948:Receptors, Antigen, T-Cell / Q000097:blood* / Q000235:genetics; D012044:Regression Analysis; D056910:Republic of Korea; D019914:Taq Polymerase; D055815:Young Adult
chemical_list: D011948:Receptors, Antigen, T-Cell; D019914:Taq Polymerase
keywords: Age estimation; Blood; Korean; sjTREC