title: Shear Stress Enhances Chemokine Secretion fromChlamydia pneumoniae-infected Monocytes.
abstract: Chlamydia pneumoniaeis a common respiratory pathogen that is considered a highly likely risk factor for atherosclerosis. C. pneumoniae is disseminated from the lung into systemic circulation via infected monocytes and lodges at the atherosclerotic sites. During transit, C.pneumoniae-infected monocytes in circulation are subjected to shear stress due to blood flow. The effect of mechanical stimuli on infected monocytes is largely understudied in the context ofC. pneumoniaeinfection and inflammation. We hypothesized that fluid shear stress alters the inflammatory response ofC. pneumoniae-infected monocytes and contributes to immune cell recruitment to the site of tissue damage. Using anin vitromodel of blood flow, we determined that a physiological shear stress of 7.5 dyn/cm2for 1 h onC. pneumoniae-infected monocytes enhances the production of several chemokines, which in turn is correlated with the recruitment of significantly large number of monocytes. Taken together, these results suggest synergistic interaction between mechanical and chemical factors inC. pneumoniaeinfection and associated inflammation.
authors: Department of Biomedical Engineering, and South Texas Center for Emerging Infectious Diseases, The University of Texas at San Antonio, San Antonio, TX 78249, USA.
authors: Shankar J
authors: SJ
authors: Evani
authors: Department of Biomedical Engineering, and South Texas Center for Emerging Infectious Diseases, The University of Texas at San Antonio, San Antonio, TX 78249, USA.
authors: Shatha F
authors: SF
authors: Dallo
authors: Departments of Pathology and Dental Medicine, Midwestern University, Downers Grove, IL 60515, USA.
authors: Ashlesh K
authors: AK
authors: Murthy
authors: Department of Biomedical Engineering, and South Texas Center for Emerging Infectious Diseases, The University of Texas at San Antonio, San Antonio, TX 78249, USA.
authors: Anand K
authors: AK
authors: Ramasubramanian
keywords: Chemokines; Chemotaxis; Chlamydia pneumoniae; Monocytes; Shear stress