title: Curcumin prevents bile canalicular alterations in the liver of hamsters infected with Opisthorchis viverrini.
abstract: Opisthorchis viverrini infection causes inflammation and liver injury leading to periductal fibrosis. Little is known about the pathological alterations in bile canaliculi in opisthorchiasis. This study aimed to investigate bile canalicular alterations in O. viverrini-infected hamsters and to examine the chemopreventive effects of curcumin on such changes. Hamsters were infected with O. viverrini and one group of animals was fed with 1% dietary curcumin supplement. Animals were examined during the acute infection phase, days 21 and 30 post-infection (PI) and chronic infection phase (day 90 PI). Scanning electron microscopy revealed that in the infected group fed with a normal diet, bile canaliculi became slightly tortuous by 30 day PI and more tortuous at day 90 PI. Transmission electron microscopy showed a reduction in microvilli density of canaliculi starting at day 30 PI, with a marked loss of microvilli at day 90 PI. These ultrastructral changes were slightly seen at day 21 PI, which was similar to that found in infected animals fed with 1% curcumin-supplemented diet. Notably, curcumin treatment prevented the reduction of microvilli density, reduced the dilation of bile canaliculi, and decreased the tortuosity of the bile canaliculi relative to non-infected animals on a normal diet at days 30 and 90 PI. These results suggest that curcumin reduces alteration of bile canaliculi and may be a promising agent to prevent the onset of bile duct abnormalities induced by O. viverrini infection.
authors: Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Prapaporn
authors: P
authors: Jattujan
authors: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. ; Liver Fluke and Cholangiocarcinoma Research Center, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Somchai
authors: S
authors: Pinlaor
authors: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. ; Liver Fluke and Cholangiocarcinoma Research Center, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Lakhanawan
authors: L
authors: Charoensuk
authors: Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Channarong
authors: C
authors: Arunyanart
authors: Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Jariya Umka
authors: JU
authors: Welbat
authors: Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Wunnee
authors: W
authors: Chaijaroonkhanarak
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000008:administration & dosage*; D001648:Bile Canaliculi / Q000473:pathology* / Q000648:ultrastructure; D018890:Chemoprevention / Q000379:methods; D006224:Cricetinae; D003474:Curcumin / Q000008:administration & dosage*; D004195:Disease Models, Animal; D004583:Electrons; D008099:Liver / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D008647:Mesocricetus; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D009889:Opisthorchiasis / Q000469:parasitology / Q000473:pathology* / Q000517:prevention & control*; D009891:Opisthorchis / Q000254:growth & development*
chemical_list: D000871:Anthelmintics; D003474:Curcumin
keywords: Opisthorchis viverrini; bile canaliculus; curcumin; hamster liver; ultrastructure