title: Role of mitochondrial calcium uniporter in regulating mitochondrial fission in the cerebral cortexes of living rats.
abstract: The mitochondrial calcium uniporter (MCU) transports Ca2+ from the cytoplasm to the mitochondrial matrix and thus maintains Ca2+ homeostasis. Previous studies have reported that inhibition of MCU by ruthenium red (RR) protects the brain from ischemia/reperfusion (I/R) injury and that mitochondrial fission plays an important role in I/R injury. However, it is still not known whether MCU affects mitochondrial fission. In the present study, treatment with RR was found to decrease the concentration of free calcium in the mitochondria, calcineurin enzyme activity and dynamin-related protein 1 expression, and treatment with spermine was found to have the opposite effect in organisms subjected to occlusion of the middle cerebral artery lasting 2 h followed by 24 h reperfusion. These results indicate that MCU may be related to mitochondrial fission via modulating mitochondrial Ca2+ uptake and this relationship between MCU and mitochondrial fission may protect the brain from I/R injury.
authors: Department of Anesthesiology, Affiliated Hospital of Qingdao University Medical College, Qingdao, 266003, Shandong, China.
authors: Nan
authors: N
authors: Liang
authors: Peng
authors: P
authors: Wang
authors: Shilei
authors: S
authors: Wang
authors: Shuhong
authors: S
authors: Li
authors: Yu
authors: Y
authors: Li
authors: Jinying
authors: J
authors: Wang
authors: Min
authors: M
authors: Wang
mesh_terms: D000818:Animals; D019703:Calcineurin / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism*; D002540:Cerebral Cortex / Q000473:pathology / Q000648:ultrastructure; D004195:Disease Models, Animal; D034281:Dynamins / Q000378:metabolism; D007202:Indicators and Reagents / Q000493:pharmacokinetics; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology; D008297:Male; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D063154:Mitochondrial Dynamics / Q000502:physiology*; D009422:Nervous System Diseases / Q000209:etiology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology; D012430:Ruthenium Red / Q000493:pharmacokinetics; D013096:Spermine / Q000494:pharmacology; D014851:Wakefulness / Q000502:physiology
chemical_list: D015220:Calcium Channels; D007202:Indicators and Reagents; C034703:mitochondrial calcium uniporter; D012430:Ruthenium Red; D013096:Spermine; D019703:Calcineurin; C519851:Dnm1l protein, rat; D034281:Dynamins; D002118:Calcium