title: Correlation of restenosis after rabbit carotid endarterectomy and inflammatory cytokines.
abstract: OBJECTIVE
  To establish rabbit model of restenosis after carotid endarterectomy surgery, and to study tissue inflammatory cytokines (TNF-α, IL-6) involved in restenosis.
  
  
  METHODS
  A total of 32 rabbits were randomly divided into two groups: model group and control group. The right common carotid artery in rabbits was damaged by carotid endar terectomy in model group. The tissues were harvested at different time points respectively, the pathological changes of the vascular wall after operation were observed at different time points. The changes of expression of tissue vascular wall inflammatory cytokines (TNF-α, IL-6) at different time points after the surgery was observed by RT-PCR, and the changes of serum inflammatory cytokines (TNF-α, IL -6) were detected by ELISA.
  
  
  RESULTS
  The new intima appeared after 7 days of the injury and reached the peak on 28 d which is uneven and significantly thicker than the control group (P<0.01). The tissue inflammatory cytokines (TNF-α, IL-6) were significantly increased after the rabbit common carotid artery injury, which was significant difference compared with normal control group (P<0.05).
  
  
  CONCLUSIONS
  The tissue inflammatory factors significantly increase after the rabbit carotid artery injury, which suggests the mutual concurrent effects of inflammatory cytokines can result in the proliferation of vascular restenosis.
authors: Department of Cardiovascular Thoracic Surgery, Anqiu People's Hospital, Anqiu 262100, China.
authors: Jun-Jun
authors: JJ
authors: Liang
authors: Department of Cardiovascular Thoracic Surgery, Anqiu People's Hospital, Anqiu 262100, China.
authors: Wei
authors: W
authors: Xue
authors: Department of Cardiovascular Thoracic Surgery, Anqiu People's Hospital, Anqiu 262100, China.
authors: Li-Zhi
authors: LZ
authors: Lou
authors: Department of Cardiovascular Thoracic Surgery, Anqiu People's Hospital, Anqiu 262100, China.
authors: Cheng
authors: C
authors: Liu
authors: Department of Medical Service, Anqiu People's Hospital, Anqiu 262100, China.
authors: Zhao-Fen
authors: ZF
authors: Wang
authors: Tianjin Cerebral Hospital, Tianjin 300060, China.
authors: Qing-Guo
authors: QG
authors: Li
authors: Department of Neurosurgery, Anqiu People's Hospital, Anqiu 262100, China. Electronic address: sdaqhsh@163.com.
authors: Shao-Hua
authors: SH
authors: Huang
mesh_terms: D000818:Animals; D017536:Carotid Artery, Common / Q000737:chemistry / Q000378:metabolism* / Q000601:surgery*; D016893:Carotid Stenosis / Q000378:metabolism*; D016894:Endarterectomy, Carotid / Q000379:methods*; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*
chemical_list: D015850:Interleukin-6; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha
keywords: Carotid endarterectomy; Inflammatory factors; Restenosis after angioplasty