title: Relationship between tumor and peripheral blood NPRL2 mRNA levels in patients with colorectal adenoma and colorectal cancer.
abstract: NPRL2 is a tumor suppressor gene involved in the progression of human cancer. The present study investigated whether NPRL2 expression correlates with colorectal cancer (CRC) progression. Colorectal tissue and peripheral blood samples were obtained from 62 patients with CRC, 38 patients with colorectal adenomas and 51 normal controls. NPRL2 mRNA levels in tissue samples and blood were measured using quantitative real-time PCR. NPRL2 protein expression was determined by immunohistochemistry. NPRL2 protein expression in CRCs was significantly lower than in the adenomas or normal colorectal tissue. NPRL2 mRNA expression was significantly decreased in adenomas compared with normal controls (P<0.0001) and it was further decreased in colorectal tumors compared with adenomas (P<0.0001). NPRL2 mRNA levels expression correlated with tumor stage. In addition, NPRL2 mRNA levels in the blood correlated with the levels detected in tumors. Furthermore, receiver operating characteristic (ROC) analysis showed that NPRL2 expression in blood could distinguish colorectal adenomas and CRCs from normal controls. NPRL2 mRNA expression in CRC tumor tissues and peripheral blood correlated with colorectal tumor progression. Based on our findings, we can conclude that NPRL2 mRNA blood levels could be a potentially useful marker for the detection of early stage adenomas and CRCs.
authors: Department of Gastroenterology; Second Affiliated Hospital of Harbin Medical University; Harbin, Heilongjiang PR China.
authors: Ai-yun
authors: AY
authors: Liu
authors: Department of General Surgery; Xuan Wu Hospital; Capital Medical University; Beijing, PR China.
authors: Dian-Ggang
authors: DG
authors: Liu
authors: Department of Gastroenterology; Second Affiliated Hospital of Harbin Medical University; Harbin, Heilongjiang PR China.
authors: Ya-ju
authors: YJ
authors: Du
authors: Department of Gastroenterology; Second Affiliated Hospital of Harbin Medical University; Harbin, Heilongjiang PR China.
authors: Feng-hua
authors: FH
authors: Pei
authors: Department of Gastroenterology; First Hospital of Harbin; Harbin, Heilongjiang PR China.
authors: Guang
authors: G
authors: Yang
authors: Department of Gastroenterology; Second Affiliated Hospital of Harbin Medical University; Harbin, Heilongjiang PR China.
authors: Bing-rong
authors: BR
authors: Liu
authors: Department of Gastroenterology; Second Affiliated Hospital of Harbin Medical University; Harbin, Heilongjiang PR China.
authors: Hui-tao
authors: HT
authors: Zhang
authors: Department of Gastroenterology; Second Affiliated Hospital of Harbin Medical University; Harbin, Heilongjiang PR China.
authors: Xin-hong
authors: XH
authors: Wang
authors: Department of Gastroenterology; Second Affiliated Hospital of Harbin Medical University; Harbin, Heilongjiang PR China.
authors: Yu-jing
authors: YJ
authors: Fan
authors: Department of Pathology; Second Affiliated Hospital of Harbin Medical University; Harbin, Heilongjiang PR China.
authors: Ying-zhun
authors: YZ
authors: Chen
authors: Department of Pathology, Harbin Medical University; Harbin, Heilongjiang PR China.
authors: Yang
authors: Y
authors: Jiang
authors: Department of Gastroenterology; Second Affiliated Hospital of Harbin Medical University; Harbin, Heilongjiang PR China.
authors: Jing
authors: J
authors: Chen
mesh_terms: D000236:Adenoma / Q000097:blood / Q000378:metabolism / Q000473:pathology*; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000097:blood / Q000378:metabolism / Q000473:pathology*; D018450:Disease Progression; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000097:blood / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000097:blood / Q000235:genetics / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; C492816:NPRL2 protein, human; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins
keywords: DNA mismatch repair; adenoma; biological markers; colorectal carcinoma; intensive tumor suppressor gene; nitrogen permease regulator-like 2