title: Nitric oxide photorelease from a trinuclear ruthenium nitrosyl complex and its in vitro cytotoxicity against melanoma cells.
abstract: In vitro cytotoxicity study of the [Ru3O(CH3COO)6(4-pic)2(NO)]PF6 triruthenium nitrosyl cluster (compound 1, 4-pic=4-methylpyridine) against B16F10 melanoma cell line was evaluated in the presence and absence of visible light irradiation. The nitrosyl cluster 1 showed a significant tumoricidal activity when irradiated at λ=532 nm, reducing cell viability up to 90% at a concentration of 62.5 μM. However, cell death of 60% is also observed in the dark which can be assigned to the NO release mediated by a redox reaction of the cluster in cell medium. This possibility was confirmed by amperometric detection of NO after the addition of ascorbic acid to compound 1 in phosphate buffer. A control experiment was performed with the solvated cluster [Ru3O(CH3COO)6(4-pic)2(CH3OH)]PF6 (compound 2) and no significant lowering of cell viability was observed. These results suggest that the nitrosyl cluster acts as a pro-drug, delivering NO, which is the actual active species.
authors: Departamento de Física e Química da Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café s/n, 14040-903 Ribeirão Preto, SP, Brazil.
authors: Zumira A
authors: ZA
authors: Carneiro
authors: Departamento de Física e Química da Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café s/n, 14040-903 Ribeirão Preto, SP, Brazil.
authors: Juliana C
authors: JC
authors: Biazzotto
authors: Escola de Engenharia da Universidade Presbiteriana Mackenzie, Rua da Consolação 930, 01302-907 São Paulo, SP, Brazil.
authors: Anamaria D P
authors: AD
authors: Alexiou
authors: Departamento de Física e Química da Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café s/n, 14040-903 Ribeirão Preto, SP, Brazil. Electronic address: sofian@fcfrp.usp.br.
authors: Sofia
authors: S
authors: Nikolaou
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D056831:Coordination Complexes / Q000737:chemistry / Q000494:pharmacology*; D008027:Light; D051379:Mice; D009569:Nitric Oxide / Q000737:chemistry / Q000494:pharmacology*; D020030:Nitric Oxide Donors / Q000737:chemistry / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010782:Photolysis
chemical_list: D000970:Antineoplastic Agents; D056831:Coordination Complexes; D020030:Nitric Oxide Donors; D009569:Nitric Oxide
keywords: Melanoma; Nitric oxide release; Triruthenium cluster; Visible light irradiation