title: Genetic manipulation of Streptococcus pyogenes (the Group A Streptococcus, GAS).
abstract: Streptococcus pyogenes (the Group A Streptococcus, GAS) is a Gram-positive bacterium responsible for a wide spectrum of diseases ranging from mild superficial infections (pharyngitis, impetigo) to severe, often life-threatening invasive diseases (necrotizing fasciitis, streptococcal toxic shock syndrome) in humans. This unit describes molecular techniques for the genetic manipulation of S. pyogenes with detailed protocols for transformation, gene disruption, allelic exchange, transposon mutagenesis, and genetic complementation.
authors: Department of Cell Biology and Molecular Genetics, Maryland Pathogen Research Institute, University of Maryland, College Park, Maryland.
authors: Yoann
authors: Y
authors: Le Breton
authors: Department of Cell Biology and Molecular Genetics, Maryland Pathogen Research Institute, University of Maryland, College Park, Maryland.
authors: Kevin S
authors: KS
authors: McIver
mesh_terms: D017353:Gene Deletion; D005816:Genetic Complementation Test; D005827:Genetics, Microbial / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*; D016254:Mutagenesis, Insertional; D013297:Streptococcus pyogenes / Q000235:genetics*; D014169:Transformation, Bacterial
keywords: Streptococcus pyogenes; genetic complementation; genomic DNA; mariner; plasmid; transformation; transposon mutagenesis