title: Surface-enhanced fluorescent immunoassay on 2D silver nanotriangles array.
abstract: Long range ordered silver nanotriangles array was fabricated for surface-enhanced fluorescent immunoassay in this paper. By polystyrene (PS) microspheres based LB template method, the silver nanotriangle array with about 100 nm in height was constructed on the surface of glass slide. On the surface of Ag nanotriangles array, the immune reaction of antigens and labeled antibodies was carried out. Based on the interaction of fluorophores from antibodies with the plasmon resonance from Ag nanotriangles and the enrichment effect of this patterned array, 3.11 times enhancement of the fluorescent intensity of the target antibodies was obtained. According to the fitting curve of fluorescent intensities and logarithmic concentrations of labeled antibodies from 100 pg/mL to 10 μg/mL, it concludes that the limit of detection by this Ag nanotriangles array for immune complex is 100 pg/mL. Due to the advantages of high sensitivity, good reproducibility, and convenient fabrication, the 2D silver nanotriangles array could be an exciting platform for bioassays in proteomics, drug discovery and diagnostics.
authors: State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, China; State Key Lab of Marine Coatings, 4 Jinhu Road, Qingdao 266071, China; Marine Chemical Research Institute, 4 Jinhu Road, Qingdao 266071, China.
authors: Zhishi
authors: Z
authors: Li
authors: State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, China.
authors: Zhinan
authors: Z
authors: Guo
authors: State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, China.
authors: Weidong
authors: W
authors: Ruan
authors: State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, China.
authors: Wei
authors: W
authors: Song
authors: State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, China.
authors: Xu
authors: X
authors: Wang
authors: State Key Lab of Marine Coatings, 4 Jinhu Road, Qingdao 266071, China; Marine Chemical Research Institute, 4 Jinhu Road, Qingdao 266071, China.
authors: Huajin
authors: H
authors: Wang
authors: State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, China. Electronic address: zhaob@mail.jlu.edu.cn.
authors: Bing
authors: B
authors: Zhao
mesh_terms: D000818:Animals; D003198:Computer Simulation; D005453:Fluorescence; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000378:metabolism; D007834:Lasers; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D018625:Microscopy, Atomic Force; D011137:Polystyrenes / Q000737:chemistry; D011817:Rabbits; D012235:Rhodamines / Q000378:metabolism; D012834:Silver / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013194:Staining and Labeling; D013499:Surface Properties
chemical_list: D007074:Immunoglobulin G; D011137:Polystyrenes; D012235:Rhodamines; D012834:Silver; C009434:tetramethylrhodamine isothiocyanate
keywords: Arrays; Immunoassay; Silver nanotriangle; Surface-enhanced fluorescence