title: Blu-ray disk lens as the objective of a miniaturized two-photon fluorescence microscope.
abstract: In this paper, we examine the performance of a Blu-ray disk (BD) aspheric lens as the objective of a miniaturized scanning nonlinear optical microscope. By combining a single 2D micro-electro mechanical system (MEMS) mirror as the scanner and with different tube lens pairs, the field of view (FOV) of the studied microscope varies from 59 μm × 93 μm up to 178 μm × 280 μm, while the corresponding lateral resolution varies from 0.6 μm to 2 μm for two-photon fluorescence (2PF) signals. With a 34/s video frame rate, in vivo dynamic observation of zebrafish heartbeat through 2PF of the excited green fluorescence protein (GFP) is demonstrated.
authors: Hsiang-Yu
authors: HY
authors: Chung
authors: Wei-Cheng
authors: WC
authors: Kuo
authors: Yu-Hsiang
authors: YH
authors: Cheng
authors: Che-Hang
authors: CH
authors: Yu
authors: Shih-Hsuan
authors: SH
authors: Chia
authors: Cheng-Yung
authors: CY
authors: Lin
authors: Jie-Shin
authors: JS
authors: Chen
authors: Huai-Jen
authors: HJ
authors: Tsai
authors: Andrey B
authors: AB
authors: Fedotov
authors: Anatoly A
authors: AA
authors: Ivanov
authors: Aleksei M
authors: AM
authors: Zheltikov
authors: Chi-Kuang
authors: CK
authors: Sun
mesh_terms: D016250:Compact Disks; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D007909:Lenses; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*; D008904:Miniaturization