title: Sinibacillus soli gen. nov., sp. nov., a moderately thermotolerant member of the family Bacillaceae.
abstract: Two Gram-staining-positive, rod-shaped and endospore-forming bacteria that represent a single species, designated strains GD05T and GD051, were isolated from a tropical forest soil and a hot spring sediment, respectively. Cells of both strains were facultatively anaerobic, catalase- and oxidase-positive, and could grow optimally at 50 °C, pH 8.0 and with 1 % (w/v) NaCl. Analysis of the 16S rRNA gene sequence revealed that these two isolates belonged to the family Bacillaceae, but did not show sequence similarities of more than 95% to members of other related genera. The G+C content of the genomic DNA was 43.7-44.1 mol%. The major cellular fatty acids were anteiso-C15:0, iso-C15:0, iso-C16:0 and anteiso-C17:0. The main polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the major menaquinone was MK-7. The peptidoglycan type was A1γ (meso-diaminopimelic acid direct). On the basis of this polyphasic taxonomic analysis, the novel strains represent a novel species of a new genus in the family Bacillaceae, order Bacillales, for which the name Sinibacillus soli gen. nov., sp. nov. is proposed. The type strain is GD05T (=CCTCC AB 2013105T=KCTC 33117T).
authors: Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
authors: Guiqin
authors: G
authors: Yang
authors: Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
authors: Shungui
authors: S
authors: Zhou
mesh_terms: D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D018441:Biofilms; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology; D045482:Hot Springs / Q000382:microbiology*; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014197:Trees / Q000382:microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C062629:menaquinone 7