title: [Genetic characterization analysis on epidemic rubella virus strains isolated in Liaoning from 2007 to 2012].
abstract: To analyze the genetic characterization of epidemic rubella virus strains isolated in Liaoning from 2007-2012, a total of 145 rubella virus strains were isolated using Vero/Slam cell line from the patients' throat swabs during rubella outbreaks and sporadics cases in Liaoning Province from 2007 to 2012. Fragments of 945 nucleotides containing 1E gene from 145 rubella virus isolates were amplified by RT-PCR, the PCR products were sequenced and analyzed. Based on the 739 nucleotides of 1E gene, the phylogenetic trees were constructed with 32 WHO rubella reference strains of 13 genotypes downloaded from GenBank and 145 rubella virus strains. The results showed that the 145 rubella virus strains in 2007 -2012 belonged to genotype 1E, nucleotide acids and amino acids similarities were 97.2%-100.0% and 97.6%-100.0%, respectively. Compared to the 1E reference strains(Rvi/ Dezhou.CHN/02, RVi/MYS/01), the nucleotide acids and amino acids similarities were 96.6%-99.2% and 98.2%-100.0%, respectively except for one amino acid change (Val246-Ala246) of RVi/Shenyang. Liaoning. CHN/13.11/13, and Asp262-Asn262 of RVi/Shenyang. Liaoning. CHN/13.11/4 and RVi/Liaoyang. Liaoning. CHN/26. 11/2. there had no change found in the important antigenic epitope sites, the hemagglutination inhibition and neutralization epitopes of the other rubella viruses. All the 145 strains isolated had the same amino acid change (Leu338--Phe338) in E1 protein. These findings suggested that genotype 1E of rubella virus was the predominant genotype in Liaoning province. the rubella prevailed in recent six years was mainly caused by rubella viruses genotype 1E with multi-transmission routes.
authors: Liaoning Provincial Center for Disease Control and Prevention, Shenyang 110005, China. dawangyan@lncdc.com
authors: Yan
authors: Y
authors: Wang
authors: Liaoning Provincial Center for Disease Control and Prevention, Shenyang 110005, China.
authors: Yan
authors: Y
authors: Ma
authors: Liaoning Provincial Center for Disease Control and Prevention, Shenyang 110005, China.
authors: Xiao-Ting
authors: XT
authors: Xu
authors: Da Lian Center for Disease Control and Prevention, Dalian 116021, China.
authors: Xue-Song
authors: XS
authors: Fan
authors: Da Lian Center for Disease Control and Prevention, Dalian 116021, China.
authors: Qian
authors: Q
authors: Lin
authors: An Shan Center for Disease Control and Prevention, Anshan 116021, China.
authors: Dan
authors: D
authors: Sui
authors: An Shan Center for Disease Control and Prevention, Anshan 116021, China.
authors: Ye
authors: Y
authors: Yin
authors: Liao Yang Center for Disease Control and Prevention, Liaoyang 111000, China.
authors: Feng-Tong
authors: FT
authors: Wu
authors: Liao Yang Center for Disease Control and Prevention, Liaoyang 111000, China.
authors: Bai-Ling
authors: BL
authors: Pan
authors: Ying Kou Center for Disease Control and Prevention, Yingkou 115004, China.
authors: Guang-Yuan
authors: GY
authors: Liu
authors: Ying Kou Center for Disease Control and Prevention, Yingkou 115004, China.
authors: Ji-Jian
authors: JJ
authors: Wang
authors: Liaoning Provincial Center for Disease Control and Prevention, Shenyang 110005, China.
authors: Yue
authors: Y
authors: Han
authors: Liaoning Provincial Center for Disease Control and Prevention, Shenyang 110005, China.
authors: Jun-Qiao
authors: JQ
authors: Guo
authors: Liaoning Provincial Center for Disease Control and Prevention, Shenyang 110005, China.
authors: Zhuo
authors: Z
authors: Zhao
mesh_terms: D000595:Amino Acid Sequence; D002681:China / Q000453:epidemiology; D058872:Epidemics; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012409:Rubella / Q000453:epidemiology / Q000821:virology*; D012412:Rubella virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016415:Sequence Alignment; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D014759:Viral Envelope Proteins