title: Transcriptome and gene expression analysis during flower blooming in Rosa chinensis 'Pallida'.
abstract: Rosa chinensis 'Pallida' (Rosa L.) is one of the most important ancient rose cultivars originating from China. It contributed the 'tea scent' trait to modern roses. However, little information is available on the gene regulatory networks involved in scent biosynthesis and metabolism in Rosa. In this study, the transcriptome of R. chinensis 'Pallida' petals at different developmental stages, from flower buds to senescent flowers, was investigated using Illumina sequencing technology. De novo assembly generated 89,614 clusters with an average length of 428bp. Based on sequence similarity search with known proteins, 62.9% of total clusters were annotated. Out of these annotated transcripts, 25,705 and 37,159 sequences were assigned to gene ontology and clusters of orthologous groups, respectively. The dataset provides information on transcripts putatively associated with known scent metabolic pathways. Digital gene expression (DGE) was obtained using RNA samples from flower bud, open flower and senescent flower stages. Comparative DGE and quantitative real time PCR permitted the identification of five transcripts encoding proteins putatively associated with scent biosynthesis in roses. The study provides a foundation for scent-related gene discovery in roses.
authors: Flower Research Institute of Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650205, PR China; Yunnan Flower Breeding Key Lab., Kunming Yunnan 650205, PR China.
authors: Huijun
authors: H
authors: Yan
authors: Flower Research Institute of Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650205, PR China; Yunnan Flower Breeding Key Lab., Kunming Yunnan 650205, PR China.
authors: Hao
authors: H
authors: Zhang
authors: Flower Research Institute of Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650205, PR China; Yunnan Flower Breeding Key Lab., Kunming Yunnan 650205, PR China.
authors: Min
authors: M
authors: Chen
authors: Flower Research Institute of Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650205, PR China; Yunnan Flower Breeding Key Lab., Kunming Yunnan 650205, PR China.
authors: Hongying
authors: H
authors: Jian
authors: Laboratoire BVpam, EA3061, Université de Saint-Etienne, Université de Lyon, 23 rue du Dr Michelon, Saint-Etienne F-42023, France.
authors: Sylvie
authors: S
authors: Baudino
authors: Laboratoire BVpam, EA3061, Université de Saint-Etienne, Université de Lyon, 23 rue du Dr Michelon, Saint-Etienne F-42023, France.
authors: Jean-Claude
authors: JC
authors: Caissard
authors: Laboratoire Reproduction et Développement des Plantes, UMR INRA-CNRS, Lyon1-ENSL, Ecole Normale Supérieure, 46 allée d'Italie, 69364 Lyon Cedex 07, France.
authors: Mohammed
authors: M
authors: Bendahmane
authors: Flower Research Institute of Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650205, PR China; Yunnan Flower Breeding Key Lab., Kunming Yunnan 650205, PR China.
authors: Shubin
authors: S
authors: Li
authors: Flower Research Institute of Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650205, PR China; Yunnan Flower Breeding Key Lab., Kunming Yunnan 650205, PR China.
authors: Ting
authors: T
authors: Zhang
authors: Flower Research Institute of Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650205, PR China; Yunnan Flower Breeding Key Lab., Kunming Yunnan 650205, PR China.
authors: Ningning
authors: N
authors: Zhou
authors: Flower Research Institute of Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650205, PR China; Yunnan Flower Breeding Key Lab., Kunming Yunnan 650205, PR China.
authors: Xianqin
authors: X
authors: Qiu
authors: Flower Research Institute of Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650205, PR China; Yunnan Flower Breeding Key Lab., Kunming Yunnan 650205, PR China.
authors: Qigang
authors: Q
authors: Wang
authors: Flower Research Institute of Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650205, PR China; Yunnan Flower Breeding Key Lab., Kunming Yunnan 650205, PR China. Electronic address: kxtang@hotmail.com.
authors: Kaixue
authors: K
authors: Tang
mesh_terms: D035264:Flowers / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D058977:Molecular Sequence Annotation; D005810:Multigene Family; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D027826:Rosa / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors
keywords: Digital gene expression; Rosa chinensis ‘Pallida’; Scent-related genes; Transcriptome