title: First identification of Mycobacterium avium paratuberculosis sheep strain in Argentina.
abstract: We here identified for the first time the presence of Mycobacterium avium paratuberculosis (MAP) sheep (S) strain in Argentina. IS900 polymerase chain reaction (PCR) was positive. The S strain was compared with MAP cattle (C) strains by using IS1311 PCR-restriction endonuclease analysis (PCR-REA), multiplex PCR and restriction fragment length polymorphism (RFLP) analysis.
authors: Veterinary Center of Diagnostic and Research, Faculty of Veterinary Science, University of La Plata, Chascomús, Buenos Aires, Argentina.
authors: G E
authors: GE
authors: Travería
authors: Biotechnology Institute, National Institute of Agricultural Technology, Castelar, Argentina.
authors: M
authors: M
authors: Zumarraga
authors: Biotechnology Institute, National Institute of Agricultural Technology, Castelar, Argentina.
authors: I
authors: I
authors: Etchechoury
authors: Biotechnology Institute, National Institute of Agricultural Technology, Castelar, Argentina.
authors: M I
authors: MI
authors: Romano
authors: Biotechnology Institute, National Institute of Agricultural Technology, Castelar, Argentina.
authors: A
authors: A
authors: Cataldi
authors: Veterinary Center of Diagnostic and Research, Faculty of Veterinary Science, University of La Plata, Chascomús, Buenos Aires, Argentina.
authors: M F Alvarado
authors: MF
authors: Pinedo
authors: Veterinary Research Institute, Hudcova, Brno, Czech Republic.
authors: I
authors: I
authors: Pavlik
authors: Veterinary Research Institute, Hudcova, Brno, Czech Republic.
authors: R
authors: R
authors: Pribylova
authors: Veterinary Center of Diagnostic and Research, Faculty of Veterinary Science, University of La Plata, Chascomús, Buenos Aires, Argentina.
authors: J R
authors: JR
authors: Romero
mesh_terms: D000818:Animals; D001118:Argentina; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D060885:Multiplex Polymerase Chain Reaction; D016927:Mycobacterium avium subsp. paratuberculosis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010283:Paratuberculosis / Q000175:diagnosis / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis / Q000382:microbiology*
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial
keywords: PCR; PCR-REA; paratuberculosis; sheep; typing