title: MicroRNA-210 promotes proliferation and invasion of peripheral nerve sheath tumor cells targeting EFNA3.
abstract: MicroRNA (miR) plays an important role in tumorigenesis including malignant peripheral nerve sheath tumor (MPNST). miR-210 downregulation is frequently observed in a variety of tumors. In this study, miR-210 was identified as downregulated in MPNST cells, and its potential target ephrin-A3 (EFNA3) was upregulated in them compared with neurofibroma cells using quantitative real-time (qRT)-PCR. Luciferase reporter assay further demonstrates that EFNA3 is a target of miR-210. Then it is confirmed that miR-210 can regulate EFNA3 mRNA and protein expression in MPNST ST88-14 (NF1 wild-type) and sNF96.2 (NF1 mutant type) cell lines. The functions of miR-210 in MPNST cells were investigated, and the results showed that overexpression of miR-210 increased cellular viability, colony formation, S phase percentage, and invasiveness of MPNST cells. Inversely, inhibition of miR-210 expression induced suppression of proliferation and invasion of MPNST cells. These results suggest that miR-210-mediated EFNA3 promotion of proliferation and invasion of MPNST cells plays an important role in MPNST tumorigenesis and progression. miR-210 and EFNA3 may be candidate novel therapeutic targets for MPNST.
authors: Department of Spine Surgery, The Second Xiangya Hospital of Central South University, Changsha, China.
authors: Zhengguang
authors: Z
authors: Wang
authors: Bangliang
authors: B
authors: Yin
authors: Bing
authors: B
authors: Wang
authors: Zemin
authors: Z
authors: Ma
authors: Weidong
authors: W
authors: Liu
authors: Guohua
authors: G
authors: Lv
mesh_terms: D048708:Cell Growth Processes / Q000235:genetics; D015536:Down-Regulation; D036384:Ephrin-A3 / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009455:Neurofibroma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D060888:Real-Time Polymerase Chain Reaction; D016196:S Phase / Q000235:genetics; D014162:Transfection
chemical_list: D036384:Ephrin-A3; C543800:MIRN210 microRNA, human; D035683:MicroRNAs