title: Dextran sulfate sodium (DSS)-induced colitis in mice.
abstract: Inflammatory bowel diseases (IBD), mainly comprising ulcerative colitis and Crohn's Disease, are complex and multifactorial diseases with unknown etiology. For the past 20 years, to study human IBD mechanistically, a number of murine models of colitis have been developed. These models are indispensable tools to decipher underlying mechanisms of IBD pathogenesis as well as to evaluate a number of potential therapeutics. Among various chemically induced colitis models, the dextran sulfate sodium (DSS)-induced colitis model is widely used because of its simplicity and many similarities with human ulcerative colitis. This model has both advantages and disadvantages that must be considered when employed. This protocol describes the DSS-induced colitis model, focusing on details and factors that could affect DSS-induced pathology.
authors: Center for Inflammation, Immunity, & Infection, Department of Biology, Georgia State University, Atlanta, Georgia.
authors: Benoit
authors: B
authors: Chassaing
authors: Center for Inflammation, Immunity, & Infection, Department of Biology, Georgia State University, Atlanta, Georgia.
authors: Jesse D
authors: JD
authors: Aitken
authors: Center for Inflammation, Immunity, & Infection, Department of Biology, Georgia State University, Atlanta, Georgia.
authors: Madhu
authors: M
authors: Malleshappa
authors: Department of Nutritional Sciences, The Pennsylvania State University, University Park, Pennsylvania.|Department of Medicine, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania.
authors: Matam
authors: M
authors: Vijay-Kumar
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000737:chemistry / Q000378:metabolism; D001835:Body Weight; D003092:Colitis / Q000139:chemically induced / Q000276:immunology*; D016264:Dextran Sulfate / Q000008:administration & dosage*; D004195:Disease Models, Animal; D005243:Feces / Q000737:chemistry; D005260:Female; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL
chemical_list: D015415:Biomarkers; D016264:Dextran Sulfate
keywords: chemical colitogen; inflammatory bowel disease; intestinal inflammation; myeloperoxidase; occult blood; ulcerative colitis