title: Control efficacy of an endophytic Bacillus amyloliquefaciens strain BZ6-1 against peanut bacterial Wilt, Ralstonia solanacearum.
abstract: We aimed to isolate and identify endophytic bacteria that might have efficacy against peanut bacterial wilt (BW) caused by Ralstonia solanacearum. Thirty-seven endophytic strains were isolated from healthy peanut plants in R. solanacearum-infested fields and eight showed antagonistic effects against R. solanacearum. Strain BZ6-1 with the highest antimicrobial activity was identified as Bacillus amyloliquefaciens based on morphology, biochemistry, and 16S rRNA analysis. Culture conditions of BZ6-1 were optimized using orthogonal test method and inhibitory zone diameter in dual culture plate assay reached 34.2 mm. Furthermore, main antimicrobial substances of surfactin and fengycin A homologues produced by BZ6-1 were analyzed by high performance liquid chromatography electrospray ionization tandem mass spectrometry. Finally, pot experiments were adopted to test the control efficiency of BZ6-1 against peanut BW. Disease incidence decreased significantly from 84.5% in the control to 12.1% with addition of 15 mL (10(8) cfu mL(-1)) culture broth for each seedling, suggesting the feasibility of strain BZ6-1 in the biological control of peanut plants BW.
authors: College of Environmental Science and Engineering, Yangzhou University, Yangzhou 225009, China ; Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing 210095, China.
authors: Xiaobing
authors: 0000-0002-8635-9916
authors: X
authors: Wang
authors: School of Chemistry and Environmental Engineering, Jiangsu University of Technology, Changzhou 213001, China.
authors: Guobin
authors: G
authors: Liang
mesh_terms: D010367:Arachis / Q000382:microbiology*; D001407:Bacillus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D002853:Chromatography, Liquid; D060026:Endophytes / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D043368:Ralstonia solanacearum / Q000235:genetics / Q000254:growth & development*
chemical_list: D012336:RNA, Ribosomal, 16S