title: Ratiometric detection of enzyme turnover and flavin reduction using rare-earth upconverting phosphors.
abstract: Gd4O2S:Yb:Tm rare-earth upconversion phosphors have been utilised to monitor the redox behaviour of flavin mononucleotide and report on the turnover of a flavo-protein, (pentaerythritol tetranitrate reductase). The presence of two bands separated by over 300 nm in the UCP emission spectra allows ratiometric signalling of these processes with high sensitivity.
authors: School of Chemistry, The University of Manchester, Oxford Road, Manchester, M13 9PL, UK. louise.natrajan@manchester.ac.uk.
authors: Peter
authors: P
authors: Harvey
authors: Chloë
authors: C
authors: Oakland
authors: Max D
authors: MD
authors: Driscoll
authors: Sam
authors: S
authors: Hay
authors: Louise S
authors: LS
authors: Natrajan
mesh_terms: D005486:Flavin Mononucleotide / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D053768:Metal Nanoparticles / Q000737:chemistry; D008674:Metals, Rare Earth / Q000737:chemistry*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry*
chemical_list: D008674:Metals, Rare Earth; D005486:Flavin Mononucleotide; D010088:Oxidoreductases; C098939:pentaerythritol tetranitrate reductase