title: Assessment of in vitro cyto/genotoxicity of sequentially treated electroplating effluent on the human hepatocarcinoma HuH-7 cell line.
abstract: The present study compares in vitro toxicity of electroplating effluent after the batch treatment process with that obtained after the sequential treatment process. Activated charcoal prepared from sugarcane bagasse through chemical carbonization, and tolerant indigenous bacteria, Bacillus sp. strain IST105, were used individually and sequentially for the treatment of electroplating effluent. The sequential treatment involving activated charcoal followed by bacterial treatment removed 99% of Cr(VI) compared with the batch processes, which removed 40% (charcoal) and 75% (bacteria), respectively. Post-treatment in vitro cyto/genotoxicity was evaluated by the MTT test and the comet assay in human HuH-7 hepatocarcinoma cells. The sequentially treated sample showed an increase in LC50 value with a 6-fold decrease in comet-assay DNA migration compared with that of untreated samples. A significant decrease in DNA migration and an increase in LC50 value of treated effluent proved the higher effectiveness of the sequential treatment process over the individual batch processes.
authors: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110 067, India. Electronic address: umeshbiology@gmail.com.
authors: Umesh Chandra
authors: UC
authors: Naik
authors: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110 067, India. Electronic address: mihirdasjnu@gmail.com.
authors: Mihir Tanay
authors: MT
authors: Das
authors: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110 067, India.
authors: Swati
authors: S
authors: Sauran
authors: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110 067, India. Electronic address: isthakur@hotmail.com.
authors: Indu Shekhar
authors: IS
authors: Thakur
mesh_terms: D001673:Biodegradation, Environmental; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002606:Charcoal / Q000737:chemistry; D020552:Comet Assay; D004249:DNA Damage; D004595:Electroplating; D004785:Environmental Pollutants / Q000737:chemistry / Q000633:toxicity*; D006801:Humans; D007220:Industrial Waste / Q000009:adverse effects* / Q000032:analysis; D019216:Metals, Heavy / Q000737:chemistry / Q000633:toxicity*; D009153:Mutagens / Q000737:chemistry / Q000633:toxicity*; D018508:Water Purification / Q000379:methods*
chemical_list: D004785:Environmental Pollutants; D007220:Industrial Waste; D019216:Metals, Heavy; D009153:Mutagens; D002606:Charcoal
keywords: Activated charcoal; Bacillus sp.; Chromium (VI); Cytotoxicity; Detoxification; Genotoxicity