title: Electroporation-mediated siRNA delivery into tumors.
abstract: Electroporation-mediated gene transfer (electro-transfection) is a powerful tool to introduce nucleic acid compounds such as plasmid DNAs, antisense oligonucleotides, and short interfering RNAs (siRNAs) into the cells. Electro-transfection is a physical gene transfer method that utilizes an electrostatic field generated with an electroporator apparatus. Here, we demonstrate a practical protocol for electro-transfection (electro-delivery) of siRNA into cells in vivo and further demonstrate the application of the method to cancer therapy. We successfully developed an original electrode (the plate and fork-type electrode) and used it for in vivo electro-delivery of siRNA. Vascular endothelial growth factor (VEGF), an angiogenic factor, was chosen as a model target gene. Electro-delivery of an siRNA targeting VEGF into the xenografted tumors in nude mice significantly suppressed the growth of tumors. By repeating the therapy with a chemically modified siRNA after an interval of 20 days, satisfactory anticancer effects were achieved.
authors: Division of Disease Models, Center for Neurological Disease and Cancer, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Yoshifumi
authors: Y
authors: Takei
mesh_terms: D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D018274:Electroporation / Q000379:methods*; D015316:Genetic Therapy; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000628:therapy; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection / Q000379:methods*
chemical_list: D034741:RNA, Small Interfering