title: A synthetic luciferin improves bioluminescence imaging in live mice.
abstract: Firefly luciferase is the most widely used optical reporter for noninvasive bioluminescence imaging (BLI) in rodents. BLI relies on the ability of the injected luciferase substrate D-luciferin to access luciferase-expressing cells and tissues within the animal. Here we show that injection of mice with a synthetic luciferin, CycLuc1, improves BLI with existing luciferase reporters and enables imaging in the brain that could not be achieved with D-luciferin.
authors: 1] Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, California, USA. [2].
authors: Melanie S
authors: MS
authors: Evans
authors: 1] Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA. [2] RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, Massachusetts, USA. [3].
authors: Joanna P
authors: JP
authors: Chaurette
authors: Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
authors: Spencer T
authors: ST
authors: Adams
authors: Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
authors: Gadarla R
authors: GR
authors: Reddy
authors: Department of Chemistry, University of California, Irvine, Irvine, California, USA.
authors: Miranda A
authors: MA
authors: Paley
authors: 1] Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA. [2] RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
authors: Neil
authors: N
authors: Aronin
authors: 1] Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, California, USA. [2] Department of Chemistry, University of California, Irvine, Irvine, California, USA.
authors: Jennifer A
authors: JA
authors: Prescher
authors: Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
authors: Stephen C
authors: SC
authors: Miller
mesh_terms: D000818:Animals; D052160:Benzothiazoles / Q000138:chemical synthesis*; D001692:Biological Transport; D008156:Luciferases / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods*; D051379:Mice; D008822:Mice, Transgenic; D015394:Molecular Structure; D059906:Neuroimaging / Q000379:methods*
chemical_list: D052160:Benzothiazoles; C532924:D-luciferin; D008156:Luciferases