title: Non-ribosomal phylogenetic exploration of Mollicute species: new insights into haemoplasma taxonomy.
abstract: Nine species of uncultivable haemoplasmas and several Mycoplasma species were examined by partial sequencing of two protein-encoding housekeeping genes. Partial glyceraldehyde-3-phosphate dehydrogenase (gapA) and heat shock protein 70 (dnaK) gene sequences were determined for these Mollicute species; in total nine gapA sequences and ten dnaK sequences were obtained. Phylogenetic analyses of these sequences, along with those of a broad selection of Mollicute species downloaded from GenBank, for the individual genes, and for the gapA and dnaK concatenated data set, revealed a clear separation of the haemoplasmas from other species within the Mycoplasma genus; indeed the haemoplasmas resided within a single clade which was phylogenetically detached from the pneumoniae group of Mycoplasmas. This is the first report to examine the use of gapA and dnaK, as well as a concatenated data set, for phylogenetic analysis of the haemoplasmas and other Mollicute species. These results demonstrate a distinct phylogenetic separation between the haemoplasmas and Mycoplasmas that corresponds with the biological differences observed in these species, indicating that further evaluation of the haemoplasmas' relationship with the Mycoplasma genus is required to determine whether reclassification of the haemoplasmas is necessary.
authors: School of Veterinary Sciences, University of Bristol, Langford BS40 5DU, United Kingdom. Electronic address: chelsea.hicks@bristol.ac.uk.
authors: C A E
authors: CA
authors: Hicks
authors: School of Veterinary Sciences, University of Bristol, Langford BS40 5DU, United Kingdom.
authors: E N
authors: EN
authors: Barker
authors: Department of Applied Sciences, University of the West of England, Frenchay Campus, Coldharbour Lane, Bristol BS16 1QY, United Kingdom.
authors: C
authors: C
authors: Brady
authors: School of Veterinary Sciences, University of Bristol, Langford BS40 5DU, United Kingdom.
authors: C R
authors: CR
authors: Stokes
authors: School of Veterinary Sciences, University of Bristol, Langford BS40 5DU, United Kingdom.
authors: C R
authors: CR
authors: Helps
authors: School of Veterinary Sciences, University of Bristol, Langford BS40 5DU, United Kingdom.
authors: S
authors: S
authors: Tasker
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D019143:Evolution, Molecular; D020043:Genes, Essential; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D009175:Mycoplasma Infections / Q000097:blood; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D008973:Tenericutes / Q000145:classification* / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D018840:HSP70 Heat-Shock Proteins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases
keywords: Hemotropic Mycoplasmas; Mycoplasma; Phylogeny; dnaK; gapA