title: Elucidation and biological activities of a new polysaccharide from cultured Cordyceps militaris.
abstract: A novel low-molecular-weight polysaccharide (CMP-1) with antioxidant, immunostimulatory and antitumor activities was isolated from the cultured Cordyceps militaris. The structure of CMP-1 was elucidated by a combination of physicochemical and instrumental analyses, and its average molecular weight was 4.3 kDa. The backbone of CMP-1 was composed of (1 → 4)-linked α-D-glucopyranosyl, (1 → 6)-linked β-D-glucopyranosyl and (1 → 4)-linked β-D-glucopyranosyl residues which branched at O-6. The branches consisted of (1 → 3)-linked α-L-rhamnopyranosyl terminated with (1 → )-linked α-D-glucopyranosyl residues. The ultrastructure of CMP-1 was further investigated by scanning electron microscope (SEM). The antioxidant assays showed that CMP-1 exhibited free radical-scavenging effects, ferrous ions-chelating ability and reducing power. In vitro test revealed that CMP-1 could significantly stimulate the mouse splenocyte proliferation. The cytotoxicity assay showed that CMP-1 inhibited the proliferation of HT-29, HeLa, HepG2 and K562 cells, with the IC50 values of 137.66, 162.59, 176.29 and 364.01 μg/mL, respectively.
authors: Biotechnological Institute of Chinese Materia Medica, Jinan University, Guangzhou 510632, China.
authors: Yongshuai
authors: Y
authors: Jing
authors: Biotechnological Institute of Chinese Materia Medica, Jinan University, Guangzhou 510632, China.
authors: Xinlu
authors: X
authors: Cui
authors: Department of Pharmacology, Jinan University, Guangzhou 510632, China.
authors: Zhiyan
authors: Z
authors: Chen
authors: Department of Pharmacology, Jinan University, Guangzhou 510632, China.
authors: Lijiao
authors: L
authors: Huang
authors: Department of Pharmacology, Jinan University, Guangzhou 510632, China. Electronic address: tsly@jnu.edu.cn.
authors: Liyan
authors: L
authors: Song
authors: Department of Pharmacology, Jinan University, Guangzhou 510632, China.
authors: Ting
authors: T
authors: Liu
authors: Department of Pharmacology, Jinan University, Guangzhou 510632, China.
authors: Wenjie
authors: W
authors: Lv
authors: Biotechnological Institute of Chinese Materia Medica, Jinan University, Guangzhou 510632, China. Electronic address: tyrm@jnu.edu.cn.
authors: Rongmin
authors: R
authors: Yu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D032661:Cordyceps / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000187:drug effects
chemical_list: D011134:Polysaccharides
keywords: Antioxidant activity; Antitumor activity; Cordyceps militaris; Immunostimulatory activity; Low-molecular-weight polysaccharide; Structure characterization