title: Optimization of matrix solid-phase dispersion for the rapid determination of salicylate and benzophenone-type UV absorbing substances in marketed fish.
abstract: A simple and effective method for the rapid determination of five salicylate and benzophenone-type UV absorbing substances in marketed fish is described. The method involves the use of matrix solid-phase dispersion (MSPD) prior to their determination by on-line silylation gas chromatography tandem mass spectrometry (GC-MS/MS). The parameters that affect the extraction efficiency were optimized using a Box-Behnken design method. The optimal extraction conditions involved dispersing 0.5g of freeze-dried powdered fish with 1.0g of Florisil using a mortar and pestle. This blend was then transferred to a solid-phase extraction (SPE) cartridge containing 1.0g of octadecyl bonded silica (C18), as the clean-up co-sorbent. The target analytes were then eluted with 7mL of acetonitrile. The extract was derivatized on-line in the GC injection-port by reaction with a trimethylsilylating (TMS) reagent. The TMS-derivatives were then identified and quantitated by GC-MS/MS. The limits of quantitation (LOQs) were less than 0.1ng/g.
authors: Department of Chemistry, National Central University, Chung-Li 320, Taiwan.
authors: Dung-Ying
authors: DY
authors: Tsai
authors: Department of Chemistry, National Central University, Chung-Li 320, Taiwan.
authors: Chien-Liang
authors: CL
authors: Chen
authors: Department of Chemistry, National Central University, Chung-Li 320, Taiwan. Electronic address: wanghsiending@gmail.com.
authors: Wang-Hsien
authors: WH
authors: Ding
mesh_terms: D000818:Animals; D001492:Bass / Q000378:metabolism; D001577:Benzophenones / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D005396:Fish Products / Q000032:analysis* / Q000191:economics; D005506:Food Contamination / Q000032:analysis; D048389:Gadus morhua / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D012459:Salicylates / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D012474:Salmon / Q000378:metabolism; D017747:Seafood / Q000032:analysis* / Q000191:economics; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D013473:Sunscreening Agents / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D017210:Tilapia / Q000378:metabolism
chemical_list: D001577:Benzophenones; D012459:Salicylates; D013473:Sunscreening Agents; C047723:benzophenone
keywords: Fish sample analysis; GC–MS/MS; Matrix solid-phase dispersion; On-line silylation; Organic UV absorbing substances