title: Effect of hemoperfusion using polymyxin B-immobilized fibers on acute lung injury in a rat sepsis model.
abstract: Direct hemoperfusion using polymyxin B-immobilized column (PMX-DHP) is recognized as an effective treatment for septic shock. However, whether its efficacy is limited to cardiovascular dysfunction remains unknown. Therefore, we planned to examine the effects of PMX-DHP in an acute lung injury model. [Materials and methods] Rats were assigned to either PMX-DHP group or control group (n= 7 in each). A lung injury was created by the intratracheal instillation of LPS. In PMX-DHP group, an arteriovenous extracorporeal circuit using PMX column was applied for three hours. The same procedure using a dummy column was applied in control group. The lung microcirculation was observed, and adherent leukocytes, RBC velocity, and the arterial PaO2 were calculated. Pathological changes and the wet/dry weight ratio of the lungs were examined. [Results] Adherent leukocytes and platelets to the lung venules were recognized at 3 hours, and their numbers increased over time. Treatment with PMX-DHP significantly suppressed these events and helped maintenance of the blood flow and PaO2 levels. The lung edema and the histologic damages were also suppressed. [Conclusions] PMX-DHP improved the microcirculation by suppressing leukocyte and platelet adhesion. PMX-DHP had beneficial effects in a model for acute lung injury.
authors: 1. Department of Emergency and Disaster Medicine, Juntendo University, Graduate School of Medicine, 2-1-1 Hongo Bunkyo-ku, Tokyo, 113-8421, Japan.
authors: Toshiaki
authors: T
authors: Iba
authors: 2. Department of Host Defense and Biochemical Research, Juntendo University, Graduate School of Medicine, 2-1-1 Hongo Bunkyo-ku, Tokyo, 113-8421, Japan.
authors: Isao
authors: I
authors: Nagaoka
authors: 1. Department of Emergency and Disaster Medicine, Juntendo University, Graduate School of Medicine, 2-1-1 Hongo Bunkyo-ku, Tokyo, 113-8421, Japan.
authors: Atsushi
authors: A
authors: Yamada
authors: 1. Department of Emergency and Disaster Medicine, Juntendo University, Graduate School of Medicine, 2-1-1 Hongo Bunkyo-ku, Tokyo, 113-8421, Japan.
authors: Masataka
authors: M
authors: Nagayama
authors: 1. Department of Emergency and Disaster Medicine, Juntendo University, Graduate School of Medicine, 2-1-1 Hongo Bunkyo-ku, Tokyo, 113-8421, Japan.
authors: Takahiro
authors: T
authors: Miki
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000503:physiopathology / Q000628:therapy*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004912:Erythrocytes / Q000473:pathology; D006464:Hemoperfusion; D006801:Humans; D007962:Leukocytes / Q000473:pathology; D008070:Lipopolysaccharides / Q000633:toxicity; D008833:Microcirculation / Q000187:drug effects; D010973:Platelet Adhesiveness / Q000187:drug effects; D011112:Polymyxin B / Q000737:chemistry / Q000627:therapeutic use*; D051381:Rats; D018805:Sepsis / Q000139:chemically induced / Q000503:physiopathology / Q000628:therapy*
chemical_list: D000900:Anti-Bacterial Agents; D008070:Lipopolysaccharides; D011112:Polymyxin B
keywords: acute lung injury; hemoadsorption; intravital microscope; lipopolysaccharide; polymyxin B-immobilized fibers