title: Expression of a Deschampsia antarctica Desv. polypeptide with lipase activity in a Pichia pastoris vector.
abstract: The current study isolated and characterized the Lip3F9 polypeptide sequence of Deschampsia antarctica Desv. (GeneBank Accession Number JX846628), which was found to be comprised of 291 base pairs and was, moreover, expressed in Pichia pastoris X-33 cells. The enzyme was secreted after 24 h of P. pastoris culture incubation and through induction with methanol. The expressed protein showed maximum lipase activity (35 U/L) with an optimal temperature of 37 °C. The lipase-expressed enzyme lost 50% of its specific activity at 25 °C, a behavior characteristic of a psychrotolerant enzyme. Recombinant enzyme activity was measured in the presence of ionic and non-ionic detergents, and a decrease in enzyme activity was detected for all concentrations of ionic and non-ionic detergents assessed.
authors: Laboratorio de Fisiología y Biología Molecular Vegetal, Instituto de Agroindustria, Facultad de Ciencias Agropecuarias y Forestales, Universidad de La Frontera, Casilla 54D, Temuco 4811230, Chile. claudia.rabert@ufrontera.cl.
authors: Claudia
authors: C
authors: Rabert
authors: Laboratorio de Fisiología y Biología Molecular Vegetal, Instituto de Agroindustria, Facultad de Ciencias Agropecuarias y Forestales, Universidad de La Frontera, Casilla 54D, Temuco 4811230, Chile. hilda.gutierrez@ufrontera.cl.
authors: Ana
authors: A
authors: Gutiérrez-Moraga
authors: Laboratorio de Fisiología Vegetal, Departamento de Botánica, Universidad de Concepción, Casilla 160-C, Correo 3, Concepción 3801061, Chile. anavarre@gmail.com.
authors: Alejandro
authors: A
authors: Navarrete
authors: Laboratorio de Fisiología Vegetal, Departamento de Botánica, Universidad de Concepción, Casilla 160-C, Correo 3, Concepción 3801061, Chile. darnacos@gmail.com.
authors: Darío
authors: D
authors: Navarrete-Campos
authors: Laboratorio de Fisiología y Biología Molecular Vegetal, Instituto de Agroindustria, Facultad de Ciencias Agropecuarias y Forestales, Universidad de La Frontera, Casilla 54D, Temuco 4811230, Chile. leon.bravo@ufrontera.cl.
authors: León
authors: L
authors: Bravo
authors: Vicerrectoría de Investigación y Postgrado, Universidad de La Frontera, Casilla 54D, Temuco 4811230, Chile. mgidekel@gmail.com.
authors: Manuel
authors: M
authors: Gidekel
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003902:Detergents / Q000494:pharmacology; D015870:Gene Expression; D017343:Genes, Plant; D007700:Kinetics; D008049:Lipase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008066:Lipolysis; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D010843:Pichia / Q000235:genetics*; D013696:Temperature; D064028:Tracheophyta / Q000235:genetics*
chemical_list: D003902:Detergents; D010455:Peptides; D008049:Lipase