title: Roles of MiR-101 and its target gene Cox-2 in early diagnosis of cervical cancer in Uygur women.
abstract: OBJECTIVE
  Early diagnosis is important for cervical cancer treatment. This study aimed to characteriz the microRNA profile and target gene protein levels of cervical cancers in Uygur women for application in early diagnosis.
  
  
  METHODS
  The profiles of miRNA in cervical cancer and chronic cervicitis were analyzed with miRNAmicroarray V4.0. The expression of miR-101 was detected by real-time PCR and locked nucleotide acid in situ hybridization (LNA-ISH). Cox-2 protein levels were assessed by immunohistochemistry.
  
  
  RESULTS
  The microarray identified a set of 12 miRNAs significantly decreased in cervical cancer in comparison to the control group. Quantitative RT-PCR analysis showed miR-101 to be significantly downregulated in cancer tissues (p<0.05) while LNA-ISH showed miR-101 positive rates of 80% (20/25) and 8% (5/25) (p<0.05) in the control and cervical cancer groups. Cox-2 positive rates of cervical cancer and control groups were 84% (21/25) and 8% (2/25) (p<0.05).
  
  
  CONCLUSIONS
  Use of down-regulation of miR-101 and up-regulation of Cox-2 as markers may play a role in early diagnosis of cervical cancer in Uygur women.
authors: Department of Pathology, College of Basic Medicine, Xinjiang Medical University, Urumqi, China E-mail : gulnark@126.com.
authors: Chen
authors: C
authors: Lin
authors: Fei
authors: F
authors: Huang
authors: Ya-Jing
authors: YJ
authors: Zhang
authors: Talafu
authors: T
authors: Tuokan
authors: Gulinaer
authors: G
authors: Kuerban
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics*; D002277:Carcinoma / Q000737:chemistry / Q000175:diagnosis / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D051546:Cyclooxygenase 2 / Q000032:analysis*; D055088:Early Detection of Cancer; D005260:Female; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D059467:Transcriptome; D002583:Uterine Cervical Neoplasms / Q000737:chemistry / Q000175:diagnosis / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; C534072:MIRN101 microRNA, human; D035683:MicroRNAs; D051546:Cyclooxygenase 2