title: Perkinsus chesapeaki observed in a new host, the European common edible cockle Cerastoderma edule, in the Spanish Mediterranean coast.
abstract: Histological observations showed the presence of a Perkinsus sp. parasite in Cerastoderma edule tissues for the first time in the Spanish Mediterranean coast. ITS molecular characterization by PCR-RFLP, in situ hybridization and sequencing, identified the parasite as Perkinsus chesapeaki, with a maximum identity of 99-100% with GenBank P. chesapeaki sequences from France and 97% with P. chesapeaki sequences of North American origin when BLAST analysis was carried out. Furthermore, phylogenetic studies placed the European cockle parasite in a well defined cluster together with the other European isolates. This is the first report of P. chesapeaki in the cockle C. edule.
authors: IRTA, Sant Carles de la Ràpita, Ctra. Poblenou Km 5, 43540 Tarragona, Spain; Catalonia's Aquaculture R&D and Innovation Reference Network (XRAq), Spain. Electronic address: noelia.carrasco@irta.es.
authors: N
authors: N
authors: Carrasco
authors: IRTA, Sant Carles de la Ràpita, Ctra. Poblenou Km 5, 43540 Tarragona, Spain.
authors: M
authors: M
authors: Rojas
authors: IRTA, Sant Carles de la Ràpita, Ctra. Poblenou Km 5, 43540 Tarragona, Spain.
authors: P
authors: P
authors: Aceituno
authors: IRTA, Sant Carles de la Ràpita, Ctra. Poblenou Km 5, 43540 Tarragona, Spain; Catalonia's Aquaculture R&D and Innovation Reference Network (XRAq), Spain.
authors: K B
authors: KB
authors: Andree
authors: IRTA, Sant Carles de la Ràpita, Ctra. Poblenou Km 5, 43540 Tarragona, Spain; Catalonia's Aquaculture R&D and Innovation Reference Network (XRAq), Spain.
authors: B
authors: B
authors: Lacuesta
authors: IRTA, Sant Carles de la Ràpita, Ctra. Poblenou Km 5, 43540 Tarragona, Spain; Catalonia's Aquaculture R&D and Innovation Reference Network (XRAq), Spain.
authors: M D
authors: MD
authors: Furones
mesh_terms: D056893:Alveolata; D000818:Animals; D049873:Cardiidae / Q000469:parasitology*; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013030:Spain
keywords: C. edule; In situ hybridization; P. chesapeaki; PCR-RFLP; Perkinsus sp.; Phylogeny