title: Use of Plackett-Burman design for rapid screening of nitrogen and carbon sources for the production of lipase in solid state fermentation by Yarrowia lipolytica from mustard oil cake (Brassica napus).
abstract: Mustard oil cake (Brassica napus), the residue obtained after extraction of mustard oil from mustard oil seeds, was investigated for the production of lipase under solid state fermentation (SSF) using the marine yeast Yarrowia lipolytica NCIM 3589. Process parameters such as incubation time, biomass concentration, initial moisture content, carbon source concentration and nitrogen source concentration of the medium were optimized. Screening of ten nitrogen and five carbon sources has been accomplished with the help of Plackett-Burman design. The highest lipase activity of 57.89 units per gram of dry fermented substrate (U/gds) was observed with the substrate of mustard oil cake in four days of fermentation.
authors: Department of Biotechnology, GITAM Institute of Technology, GITAM University, Gandhinagar, Visakhapatnam, AP, India. ; Center for Biotechnology, Department of Chemical Engineering, Andhra University, Visakhapatnam, AP, India.
authors: Sarat Babu
authors: SB
authors: Imandi
authors: M.R.College of Pharmacy, Phool Baugh, Vizianagaram, AP, India.
authors: Sita Kumari
authors: SK
authors: Karanam
authors: Center for Biotechnology, Department of Chemical Engineering, Andhra University, Visakhapatnam, AP, India. ; Department of Chemical Engg. & Biotechnology, Anil Neerukonda Institute of Technology and Sciences, Sangivalasa, Visakhapatnam, AP, India.
authors: Hanumantha Rao
authors: HR
authors: Garapati
mesh_terms: D018533:Biomass; D029688:Brassica napus / Q000378:metabolism* / Q000382:microbiology*; D002244:Carbon / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D003627:Data Interpretation, Statistical; D005285:Fermentation; D008049:Lipase / Q000378:metabolism*; D009149:Mustard Plant; D009584:Nitrogen / Q000378:metabolism*; D010938:Plant Oils / Q000302:isolation & purification; D013997:Time Factors; D025062:Yarrowia / Q000201:enzymology* / Q000254:growth & development*
chemical_list: D003470:Culture Media; D010938:Plant Oils; D002244:Carbon; D008049:Lipase; D009584:Nitrogen; C027793:mustard oil
keywords: Plackett-Burman design; Yarrowia lipolytica; lipase; mustard oil cake