title: Spectral density mapping protocols for analysis of molecular motions in disordered proteins.
abstract: Spectral density mapping represents the method of choice for investigations of molecular motions of intrinsically disordered proteins (IDPs). However, the current methodology has been developed for well-folded proteins. In order to find conditions for a reliable analysis of relaxation of IDPs, accuracy of the current reduced spectral density mapping protocols applied to IDPs was examined and new spectral density mapping methods employing cross-correlated relaxation rates have been designed. Various sources of possible systematic errors were analyzed theoretically and the presented approaches were tested on a partially disordered protein, delta subunit of bacterial RNA polymerase. Results showed that the proposed protocols provide unbiased description of molecular motions of IDPs and allow to separate slow exchange from fast dynamics.
authors: Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic.
authors: Pavel
authors: P
authors: Kadeřávek
authors: Vojtěch
authors: V
authors: Zapletal
authors: Alžbeta
authors: A
authors: Rabatinová
authors: Libor
authors: L
authors: Krásný
authors: Vladimír
authors: V
authors: Sklenář
authors: Lukáš
authors: L
authors: Žídek
mesh_terms: D064267:Intrinsically Disordered Proteins / Q000032:analysis* / Q000737:chemistry*; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D011487:Protein Conformation; D017510:Protein Folding
chemical_list: D064267:Intrinsically Disordered Proteins