title: Electrochemical screening of biomembrane-active compounds in water.
abstract: Interactions of biomembrane-active compounds with phospholipid monolayers on microfabricated Pt/Hg electrodes in an on-line high throughput flow system are demonstrated by recording capacitance current peak changes as rapid cyclic voltammograms (RCV). Detection limits of the compounds' effects on the layer have been estimated from the data. Compounds studied include steroids, polycyclic aromatic hydrocarbons, tricyclic antidepressants and tricyclic phenothiazines. The results show that the extent and type of interaction depends on the-(a) presence and number of aromatic rings and substituents, (b) presence and composition of side chains and, (c) molecular shape. Interaction is only indirectly related to compound hydrophobicity. For a selection of tricyclic antidepressants and tricyclic phenothiazines the detection limit in water is related to their therapeutic normal threshold. The sensing assay has been tested in the presence of humic acid as a potential interferent and in a tap water matrix. The system can be applied to the screening of putative hazardous substances and pharmaceuticals allowing for early detection thereof in the water supply. The measurements are made in real time which means that potentially toxic compounds are detected rapidly within <10 min per assay. This technology will contribute greatly to environment safety and health.
authors: School of Chemistry, University of Leeds, Leeds LS2 9JT, United Kingdom. Electronic address: cmsm@leeds.ac.uk.
authors: Shahrzad
authors: S
authors: Mohamadi
authors: School of Chemistry, University of Leeds, Leeds LS2 9JT, United Kingdom.
authors: Daniel J
authors: DJ
authors: Tate
authors: School of Chemistry, University of Leeds, Leeds LS2 9JT, United Kingdom.
authors: Alexander
authors: A
authors: Vakurov
authors: School of Chemistry, University of Leeds, Leeds LS2 9JT, United Kingdom.
authors: Andrew
authors: A
authors: Nelson
mesh_terms: D000929:Antidepressive Agents, Tricyclic / Q000032:analysis*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D057230:Limit of Detection; D008567:Membranes, Artificial; D010640:Phenothiazines / Q000032:analysis*; D010713:Phosphatidylcholines / Q000737:chemistry; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D013256:Steroids / Q000032:analysis*; D014867:Water / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D000929:Antidepressive Agents, Tricyclic; D008567:Membranes, Artificial; D010640:Phenothiazines; D010713:Phosphatidylcholines; D011084:Polycyclic Aromatic Hydrocarbons; D013256:Steroids; D014874:Water Pollutants, Chemical; D014867:Water; C017251:1,2-oleoylphosphatidylcholine; C031637:phenothiazine
keywords: Flow cell; Mercury film electrode; Phospholipid monolayer; Wafer based sensing device