title: N-terminus oligomerization is conserved in intracellular calcium release channels.
abstract: Oligomerization of all three mammalian ryanodine receptor isoforms, a structural requirement for normal intracellular Ca2+ release channel function, is displayed by the discrete N-terminal domain which assembles into homo- and hetero-tetramers. This is demonstrated in yeast, mammalian cells and native tissue by complementary yeast two-hybrid, chemical cross-linking and co-immunoprecipitation assays. The IP3 (inositol 1,4,5-trisphosphate) receptor N-terminus (residues 1-667) similarly exhibits tetrameric association as indicated by chemical cross-linking and co-immunoprecipitation assays. The presence of either a 15-residue splice insertion or of the cognate ligand IP3 did not affect tetramerization of the IP3 receptor N-terminus. Thus N-terminus tetramerization appears to be an essential intrinsic property that is conserved in both the ryanodine receptor and IP3 receptor families of mammalian intracellular Ca2+ release channels.
authors: *Wales Heart Research Institute, Cardiff University School of Medicine, Institute of Molecular and Experimental Medicine, Heath Park, Cardiff CF14 4XN, U.K.
authors: Spyros
authors: S
authors: Zissimopoulos
authors: *Wales Heart Research Institute, Cardiff University School of Medicine, Institute of Molecular and Experimental Medicine, Heath Park, Cardiff CF14 4XN, U.K.
authors: Jason
authors: J
authors: Marsh
authors: *Wales Heart Research Institute, Cardiff University School of Medicine, Institute of Molecular and Experimental Medicine, Heath Park, Cardiff CF14 4XN, U.K.
authors: Laurence
authors: L
authors: Stannard
authors: *Wales Heart Research Institute, Cardiff University School of Medicine, Institute of Molecular and Experimental Medicine, Heath Park, Cardiff CF14 4XN, U.K.
authors: Monika
authors: M
authors: Seidel
authors: *Wales Heart Research Institute, Cardiff University School of Medicine, Institute of Molecular and Experimental Medicine, Heath Park, Cardiff CF14 4XN, U.K.
authors: F Anthony
authors: FA
authors: Lai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018076:DNA, Complementary; D005786:Gene Expression Regulation / Q000502:physiology; D057809:HEK293 Cells; D006801:Humans; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000235:genetics / Q000378:metabolism; D021122:Protein Subunits; D011817:Rabbits; D019837:Ryanodine Receptor Calcium Release Channel / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D018076:DNA, Complementary; D053496:Inositol 1,4,5-Trisphosphate Receptors; D021122:Protein Subunits; D019837:Ryanodine Receptor Calcium Release Channel