title: Metabolism of secondary metabolites isolated from Tartary buckwheat and its extract.
abstract: The metabolism of Tartary buckwheat was investigated using a strategy from single bioactive compounds to complex Tartary buckwheat extract. Firstly, the metabolites of different structural compounds were investigated by an in situ liver-intestinal perfusion model and the metabolic pathways were proposed. Furthermore, Tartary buckwheat extract in rats was elucidated on the basis of the metabolism information of single compounds. High-performance liquid chromatography coupled with high resolution mass and multiple-stage mass spectrometry (HPLC-HRMS/MS(n)) was performed to characterise and identify 19 metabolites in perfusate and intestinal content after administration of single compounds to an in situ liver/intestinal perfusion model and 16 metabolites and 6 components in rat faeces, urine, bile, and plasma after oral administration of Tartary buckwheat to rats. Five new metabolites were identified as the glucuronidation and sulfation products of N-trans-feruloyltyramine and the methylation product of quercetin-3-O-[β-d-xyloxyl-(1→2)-α-l-rhamnoside]. The metabolic pathways of phenylpropanoid glycosides and N-trans-feruloyltyramine were proposed for the first time.
authors: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100050, China.
authors: Qiang
authors: Q
authors: Ren
authors: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100050, China.
authors: Yingfei
authors: Y
authors: Li
authors: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100050, China.
authors: Caisheng
authors: C
authors: Wu
authors: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100050, China.
authors: Caihong
authors: C
authors: Wang
authors: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100050, China.
authors: Ying
authors: Y
authors: Jin
authors: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100050, China. Electronic address: zhjl@imm.ac.cn.
authors: Jinlan
authors: J
authors: Zhang
mesh_terms: D000818:Animals; D001646:Bile / Q000737:chemistry / Q000378:metabolism; D019612:Fagopyrum / Q000737:chemistry / Q000378:metabolism*; D005243:Feces / Q000737:chemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000737:chemistry; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D008297:Male; D015394:Molecular Structure; D010936:Plant Extracts / Q000097:blood / Q000737:chemistry* / Q000378:metabolism* / Q000652:urine; D051381:Rats; D017208:Rats, Wistar; D064210:Secondary Metabolism
chemical_list: D010936:Plant Extracts
keywords: HPLC-HRMS/MS(n); Identification; Liver-intestinal perfusion; Metabolites; Tartary buckwheat