title: Exosome-formed synthetic microRNA-143 is transferred to osteosarcoma cells and inhibits their migration.
abstract: MicroRNAs (miRNAs) have emerged as potential anticancer agents, but their clinical application is limited by the lack of an effective delivery system to tumors. Exosomes are small vesicles that play important roles in intercellular communication. Here, we show that synthetic miR-143 introduced into cells is released enveloped in exosomes and that the secreted exosome-formed miR-143 is transferred to osteosarcoma cells. The delivery of exosome-formed miR-143 significantly reduced the migration of osteosarcoma cells. The delivery efficiency of exosome-formed miR-143 was less than that achieved with lipofection, but the migratory potential of osteosarcoma cells was similarly inhibited after both strategies. Our results suggest that exosomes can deliver synthetic miR-143 and are a potentially efficient and functional delivery system.
authors: Department of Orthopedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan. Electronic address: k_s08_10hyogo@hotmail.com.
authors: Keisuke
authors: K
authors: Shimbo
authors: Department of Orthopedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan; Department of Regenerative Medicine, Hiroshima University Hospital, Hiroshima, Japan.
authors: Shigeru
authors: S
authors: Miyaki
authors: Department of Regenerative Medicine, Hiroshima University Hospital, Hiroshima, Japan.
authors: Hiroyuki
authors: H
authors: Ishitobi
authors: Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, Japan.
authors: Yoshio
authors: Y
authors: Kato
authors: Department of Orthopedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan.
authors: Tadahiko
authors: T
authors: Kubo
authors: Department of Orthopedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan.
authors: Shoji
authors: S
authors: Shimose
authors: Department of Orthopedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan.
authors: Mitsuo
authors: M
authors: Ochi
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D055354:Exosomes / Q000378:metabolism*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D035683:MicroRNAs / Q000008:administration & dosage* / Q000235:genetics / Q000378:metabolism / Q000627:therapeutic use; D009362:Neoplasm Metastasis / Q000473:pathology / Q000517:prevention & control*; D012516:Osteosarcoma / Q000235:genetics / Q000473:pathology* / Q000628:therapy; D014162:Transfection
chemical_list: C542359:MIRN143 microRNA, human; D035683:MicroRNAs
keywords: Delivery system; Exosome; Osteosarcoma cells; miR-143