title: Development of an automated culture system for laboratory evolution.
abstract: Laboratory evolution provides phenotype-genotype mappings and quantitative analysis of selective pressures, giving important insights about evolutionary dynamics. Moreover, parallel laboratory evolution clarifies which phenotypic and genotypic changes are inevitable for adaptive evolution. Such parallel experiments, however, remain labor-intensive. In this study, to facilitate massive parallel laboratory evolution, we developed an automated culture system that can maintain hundreds of independent culture series in exponential growth phase under various culture conditions. We demonstrate the performance of this automated culture system using the laboratory evolution of Escherichia coli under various stressors.
authors: Quantitative Biology Center (QBiC), RIKEN, Osaka, Japan.
authors: Takaaki
authors: T
authors: Horinouchi
authors: Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, Osaka, Japan.
authors: Teruaki
authors: T
authors: Minamoto
authors: Quantitative Biology Center (QBiC), RIKEN, Osaka, Japan.
authors: Shingo
authors: S
authors: Suzuki
authors: Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, Osaka, Japan.
authors: Hiroshi
authors: H
authors: Shimizu
authors: Quantitative Biology Center (QBiC), RIKEN, Osaka, Japan Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, Osaka, Japan chikara.furusawa@riken.jp.
authors: Chikara
authors: C
authors: Furusawa
mesh_terms: D057205:Automation, Laboratory / Q000295:instrumentation* / Q000379:methods*; D001431:Bacteriological Techniques / Q000295:instrumentation* / Q000379:methods*; D019020:Directed Molecular Evolution; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development*
keywords: Escherichia coli; automated culture system; laboratory evolution