title: Methane production from marine microalgae Isochrysis galbana.
abstract: Methane production from marine microalgae Isochrysis galbana was assessed before and after mechanical and chemical pretreatments. Mechanical pretreatment resulted in a 61.7% increase in soluble Chemical Oxygen Demand. Different hydrolysis conditions were evaluated by varying temperature - T, sulfuric acid concentration - AC and biomass suspension concentration (measured as particulate COD - CODp) using an experimental design. The most significant interaction occurred between AC and T and the hydrolysis condition that showed the best result in the anaerobic digestion step was the condition at 40°C with addition of 0.2% (v/v) acid for 16h (9.27LCH4/kgVS). The low methane yields were attributed to inhibitory sodium concentrations for anaerobic digestion. Eliminating inhibitory sodium in the anaerobic digestion by biomass prewashing, there was a 71.5% increase in methane yield for biomass after acid hydrolysis, demonstrating the need for pretreatment and reduction in sodium concentration in the anaerobic digestion.
authors: Department of Biochemical Engineering, School of Chemistry, Federal University of Rio de Janeiro, Cidade Universitária, Centro de Tecnologia, Bl. E, Sl. 203, Ilha do Fundão, 21941-909 Rio de Janeiro, Brazil.
authors: Nathalia O
authors: NO
authors: Santos
authors: Department of Biochemical Engineering, School of Chemistry, Federal University of Rio de Janeiro, Cidade Universitária, Centro de Tecnologia, Bl. E, Sl. 203, Ilha do Fundão, 21941-909 Rio de Janeiro, Brazil.
authors: Suzana M
authors: SM
authors: Oliveira
authors: Petroleum Engineering, Estácio de Sá University, Av. Presidente Vargas, 2560, Centro, 20210-030 Rio de Janeiro, Brazil. Electronic address: larissacalves@yahoo.com.br.
authors: Larissa C
authors: LC
authors: Alves
authors: Department of Biochemical Engineering, School of Chemistry, Federal University of Rio de Janeiro, Cidade Universitária, Centro de Tecnologia, Bl. E, Sl. 203, Ilha do Fundão, 21941-909 Rio de Janeiro, Brazil. Electronic address: christe@eq.ufrj.br.
authors: Magali C
authors: MC
authors: Cammarota
mesh_terms: D000693:Anaerobiosis / Q000187:drug effects; D059001:Aquatic Organisms / Q000187:drug effects / Q000378:metabolism*; D001673:Biodegradation, Environmental / Q000187:drug effects; D056804:Biofuels / Q000382:microbiology; D057919:Biological Oxygen Demand Analysis; D018533:Biomass; D002245:Carbon Dioxide / Q000378:metabolism; D058087:Haptophyta / Q000187:drug effects / Q000378:metabolism*; D006868:Hydrolysis / Q000187:drug effects; D008697:Methane / Q000096:biosynthesis*; D058086:Microalgae / Q000187:drug effects / Q000378:metabolism*; D054712:Salinity; D012964:Sodium / Q000494:pharmacology; D013464:Sulfuric Acids / Q000494:pharmacology; D013696:Temperature
chemical_list: D056804:Biofuels; D013464:Sulfuric Acids; D002245:Carbon Dioxide; D012964:Sodium; C033158:sulfuric acid; D008697:Methane
keywords: Anaerobic digestion; Biogas; Isochrysis galbana; Marine microalgae; Thermal pre-treatment