title: Efficacy of formalin-killed Pseudomonas anguilliseptica vaccine on immune gene expression and protection in farmed olive flounder, Paralichthys olivaceus.
abstract: Formalin killed Pseudomonas anguilliseptica bacterial vaccine was prepared and administered to farm reared olive flounder, Paralichthys olivaceus reared at 17 °C and 20 °C for 4 weeks. Non-vaccinated fishes (n=150) served as positive control. Vaccinated fishes were divided into two groups (n=150 each in replicate). Both the vaccinated and non-vaccinated fishes were challenged intraperitoneally with P. anguilliseptica (1×10(7) CFU ml(-1)) isolates and PBS (negative control). Fishes were sampled from zero hour post injection (hpi) for 28 days (each hour and each day); the mean percent mortality and relative percent survival (RPS) were calculated for the challenged and control groups. The vaccinated fishes had a significant increase in RPS (69 and 89, respectively); the percentage mortality declined from 83±0.6 and 74±0.7 in challenged and control fishes to 25%±0.8% and 8%±0.8% in vaccinated and challenged fish groups, respectively. The immune gene expression assay was analyzed using real-time PCR. Vaccinated fishes registered a significant increase in the expression of TNFR-1, FasL, IRF7, TLR2, IL-1b and CD40 gene transcripts when compared to the control group. The upregulation of these genes along with the increased RPS values suggest that the formalin-killed cells of P. anguilliseptica could play an important role in immunizing olive flounder against P. anguilliseptica.
authors: Jeju Special Self-Governing Province Ocean and Fisheries Research Institute, Pyoseon-myeon, Segwipo-si, Jeju 697-914, South Korea.
authors: Yeoung-Hwan
authors: YH
authors: Jang
authors: Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences & Marine and Environmental Research Institute, Jeju National University, Jeju 690-756, South Korea.
authors: Dharaneedharan
authors: D
authors: Subramanian
authors: Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences & Marine and Environmental Research Institute, Jeju National University, Jeju 690-756, South Korea. Electronic address: msheo@jejunu.ac.kr.
authors: Moon-Soo
authors: MS
authors: Heo
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001428:Bacterial Vaccines / Q000276:immunology / Q000627:therapeutic use*; D005393:Fish Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D005432:Flounder / Q000276:immunology* / Q000382:microbiology; D005557:Formaldehyde; D005786:Gene Expression Regulation; D011549:Pseudomonas / Q000276:immunology; D011552:Pseudomonas Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D014611:Vaccination / Q000662:veterinary
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D005557:Formaldehyde
keywords: Immune gene expression; Jeju Island; Olive flounder; Pseudomonas anguilliseptica; Winter diseases