title: Evaluation of antibacterial and cytotoxic effects of nano-sized bioactive glass/collagen composites releasing tetracycline hydrochloride.
abstract: OBJECTIVE
  To evaluate the antibacterial efficacy of silicate bioactive glass nanoparticles/collagen composites functionalized with tetracycline hydrochloride (TCH).
  
  
  RESULTS
  Different concentrations of tetracycline hydrochloride (TCH) were incorporated on silicate bioactive glass nanoparticles/collagen composites by dipping these biomaterials for 48 h at 37°C in a solution of simulated body fluid (SBF) plus 0·05, 0·20 or 0·35 mg ml(-1) of the antibiotic. TCH release was assessed in double-distilled water at 37°C up to 72 h. The antibacterial activity of the samples has been evaluated in two ways: inhibition zone test and plate count method. The experiments were performed in vitro up to 48 h on four staphylococci strains (Staphylococcus aureus ATCC29213, ATCC25923, ATCC6538P and Staphylococcus epidermidis ATCC12228). The new composites were also tested for cytotoxicity on MG-63 human osteosarcoma cells. The results showed that the incorporation and release of TCH was dependent on the initial concentration of TCH in SBF. The biomaterials also inhibited the Staph. aureus cell growth even though the efficacy was similar for all concentration. On the other hand, no cytotoxic effects were found on osteoblast-like cells, even at the highest concentration.
  
  
  CONCLUSIONS
  Considering all results, it can be concluded that the new composite acts as a suitable bioactive carrier of TCH and could have potential in the prevention of biomaterial related infections.
  
  
  CONCLUSIONS
  The results suggest a potential application as wound dressing.
authors: Grupo Interdisciplinario en Materiales- Universidad Católica de Salta (IESIING-UCASAL), Instituto de Tecnologías y Ciencias de Ingeniería-Universidad Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (INTECIN UBA-CONICET), Salta, Argentina.
authors: J
authors: J
authors: Rivadeneira
authors: A L
authors: AL
authors: Di Virgilio
authors: M C
authors: MC
authors: Audisio
authors: A R
authors: AR
authors: Boccaccini
authors: A A
authors: AA
authors: Gorustovich
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D001826:Body Fluids; D045744:Cell Line, Tumor; D003094:Collagen / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D006801:Humans; D053761:Nanocomposites / Q000737:chemistry*; D010006:Osteoblasts / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D013212:Staphylococcus epidermidis / Q000187:drug effects; D013752:Tetracycline / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D003094:Collagen; D013752:Tetracycline
keywords: antibacterial activity; antibiotic; bioactive glass; collagen