title: High UBCH10 protein expression as a marker of poor prognosis in esophageal squamous cell carcinoma.
abstract: OBJECTIVE
  Ubiquitin-conjugating enzyme H10 (UBCH10) is required in the cell-cycle transition from metaphase to anaphase. Therefore, we investigated whether its expression level in cancerous esophageal lesions affected prognosis of patients with esophageal squamous-cell carcinoma.
  
  
  METHODS
  Paraffin-embedded tissue samples from 121 patients with esophageal squamous cell carcinoma were stained with antibody to UBCH10 for immunohistochemical analysis.
  
  
  RESULTS
  UBCH10 was expressed in cancerous and dysplastic lesions, but not in normal tissue. Patients were grouped according to expression: High (N=33) or low (N=88), depending on the staining pattern. There were significant differences between the groups in terms of invasion into lymphatic vessels, number of metastatic lymph nodes, TNM classification, and stages, as well as in survival: the 50% survival rate in the high expression group was 2.3 years, whereas it was 9.9 years for the low-expression group (p<0.0001). Even with multivariate adjusting for stage 0 to stage IV using the Cox proportional hazard model, patients belonging to the high-expression group had a poor prognosis (Hazard ratio=2.5; 95% Confidence Interval=1.3-4.5; p=0.004).
  
  
  CONCLUSIONS
  High protein expression of UBCH10 is a marker of poor prognosis in esophageal squamous cell carcinoma.
authors: Department of Surgery, Jikei University School of Medicine, Nishi-shimbashi 3 - 25 - 8, Minato-ku, Tokyo, 105-8461, Japan. ii3a-mtmt@asahi-net.or.jp.
authors: Akira
authors: A
authors: Matsumoto
authors: Yoshio
authors: Y
authors: Ishibashi
authors: Mitsuyoshi
authors: M
authors: Urashima
authors: Nobuo
authors: N
authors: Omura
authors: Koji
authors: K
authors: Nakada
authors: Katsunori
authors: K
authors: Nishikawa
authors: Atsuo
authors: A
authors: Shida
authors: Koji
authors: K
authors: Takada
authors: Hideyuki
authors: H
authors: Kashiwagi
authors: Katsuhiko
authors: K
authors: Yanaga
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000096:biosynthesis; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000473:pathology; D004938:Esophageal Neoplasms / Q000201:enzymology* / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D016612:Paraffin Embedding; D011379:Prognosis; D016016:Proportional Hazards Models; D012189:Retrospective Studies; D044763:Ubiquitin-Conjugating Enzymes / Q000096:biosynthesis*
chemical_list: D014408:Biomarkers, Tumor; C105156:UBE2C protein, human; D044763:Ubiquitin-Conjugating Enzymes
keywords: Esophageal squamous cell carcinoma; UBCH10; ubiquitin-conjugating enzymes; ubiquitin-proteasome pathway