title: New insight into the binding interaction of hydroxylated carbon nanotubes with bovine serum albumin.
abstract: In order to understand the effects of carbon nanotubes on the structural stability of proteins, the ligand-binding ability, fibrillation, and chemical denaturation of bovine serum albumin in the presence of a multi-walled hydroxylated carbon nanotubes (HO-MWCNTs) was characterized by UV-vis, circular dichroism, fluorescence spectroscopy and molecule modeling methods at the molecular level. The experiment results indicated that the fluorescence intensity of BSA was decreased obviously in presence of HO-MWCNTs. The binding interaction of HO-MWCNTs with BSA led to the secondary structure changes of BSA. This interaction could not only affect the ligand-binding ability of BSA, but also change the rate of fibrillation and denaturation of BSA. This work gave us some important information about the structures and properties of protein induced by carbon nanotubes.
authors: Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province 224002, People's Republic of China.
authors: Yonghui
authors: Y
authors: Guan
authors: Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province 224002, People's Republic of China.
authors: Hongmei
authors: H
authors: Zhang
authors: Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province 224002, People's Republic of China. Electronic address: wyqing76@126.com.
authors: Yanqing
authors: Y
authors: Wang
mesh_terms: D000818:Animals; D052160:Benzothiazoles; D002417:Cattle; D002942:Circular Dichroism; D019791:Guanidine / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D006900:Hydroxylation; D007700:Kinetics; D062105:Molecular Docking Simulation; D037742:Nanotubes, Carbon / Q000737:chemistry*; D066329:Protein Aggregates / Q000187:drug effects; D011485:Protein Binding / Q000187:drug effects; D011489:Protein Denaturation / Q000187:drug effects; D017433:Protein Structure, Secondary; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013844:Thiazoles / Q000378:metabolism
chemical_list: D052160:Benzothiazoles; D037742:Nanotubes, Carbon; D066329:Protein Aggregates; D013844:Thiazoles; C009462:thioflavin T; D012710:Serum Albumin, Bovine; D019791:Guanidine
keywords: Binding mechanism; Bovine serum albumin; Conformational changes; Fluorescence spectroscopy; Multi-walled hydroxylated carbon nanotubes