title: Epidermal stem cells cultured on collagen-modified chitin membrane induce in situ tissue regeneration of full-thickness skin defects in mice.
abstract: A Large scale of full-thickness skin defects is lack of auto-grafts and which requires the engineered skin substitutes for repair and regeneration. One major obstacle in skin tissue engineering is to expand epidermal stem cells (ESCs) and develop functional substitutes. The other one is the scaffold of the ESCs. Here, we applied type I collagen-modified chitin membrane to form collagen-chitin biomimetic membrane (C-CBM), which has been proved to have a great biocompatibility and degraded totally when it was subcutaneously transplanted into rat skin. ESCs were cultured, and the resulting biofilm was used to cover full-thickness skin defects in nude mice. The transplantation of ESCs- collagen- chitn biomimetic membrane (ESCs-C-CBM) has achieved in situ skin regeneration. In nude mice, compared to controls with collagen-chitin biomimetic membrane (C-CBM) only, the ESCs-C-CBM group had significantly more dermatoglyphs on the skin wound 10 w after surgery, and the new skin was relatively thick, red and elastic. In vivo experiments showed obvious hair follicle cell proliferation in the full-thickness skin defect. Stem cell markers examination showed active ESCs in repair and regeneration of skin. The results indicate that the collagen-modified chitin membrane carry with ESCs has successfully regenerated the whole skin with all the skin appendages and function.
authors: Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital Medical College, Jinan University, Guangzhou, People's Republic of China.
authors: Yan
authors: Y
authors: Shen
authors: Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital Medical College, Jinan University, Guangzhou, People's Republic of China.
authors: Libing
authors: L
authors: Dai
authors: Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital Medical College, Jinan University, Guangzhou, People's Republic of China.
authors: Xiaojian
authors: X
authors: Li
authors: Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital Medical College, Jinan University, Guangzhou, People's Republic of China.
authors: Rong
authors: R
authors: Liang
authors: Department of Medical Laboratory, Second Affiliated Hospital of Guangzhou Medical College, Guangzhou, People's Republic of China.
authors: Guangxiong
authors: G
authors: Guan
authors: Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital Medical College, Jinan University, Guangzhou, People's Republic of China.
authors: Zhi
authors: Z
authors: Zhang
authors: Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital Medical College, Jinan University, Guangzhou, People's Republic of China.
authors: Wenjuan
authors: W
authors: Cao
authors: Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital Medical College, Jinan University, Guangzhou, People's Republic of China.
authors: Zhihe
authors: Z
authors: Liu
authors: Student of Sophie Davis School of Biomedical Education, Mack Lipkin Fellowship, New York, New York, United States of America.
authors: Shirley
authors: S
authors: Mei
authors: Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital Medical College, Jinan University, Guangzhou, People's Republic of China.
authors: Weiguo
authors: W
authors: Liang
authors: Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital Medical College, Jinan University, Guangzhou, People's Republic of China.
authors: Shennan
authors: S
authors: Qin
authors: School of Pathology and Laboratory Medicine, The University of Western Australia, Nedlands, Western Australia, Australia.
authors: Jiake
authors: J
authors: Xu
authors: Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital Medical College, Jinan University, Guangzhou, People's Republic of China.
authors: Honghui
authors: H
authors: Chen
mesh_terms: D000818:Animals; D002686:Chitin; D004817:Epidermis; D051379:Mice; D012038:Regeneration / Q000502:physiology*; D012879:Skin Physiological Phenomena; D016038:Skin Transplantation / Q000379:methods*; D017768:Skin, Artificial; D033581:Stem Cell Transplantation / Q000379:methods*; D014945:Wound Healing
chemical_list: D002686:Chitin