title: Simultaneous determination of loratadine, desloratadine and cetirizine by capillary zone electrophoresis.
abstract: OBJECTIVE
  The aim of the study was the development of a simple and rapid analytical procedure for the determination of the most frequently used antihistamine derivatives.
  
  
  METHODS
  A capillary zone electrophoretic method was developed for the simultaneous separation of loratadine, desloratadine and cetirizine. Efforts were focused primarly on the optimisation of the experimental parameters: buffer composition and concentration, buffer pH, applied voltage, temperature, injection pressure and time.
  
  
  RESULTS
  The optimised parameters for the separation were: 25 mM buffer electrolyte, buffer pH 2.5, voltage + 25 kV, temperature 25 °C, injection pressure 50 mbar, injection time 3 seconds, capillary 48 cm (effective length 40 cm) x 50 μm, detection at 240 nm. Under these conditions, the analysis time was below 5 minutes, the order of migration being: desloratadine, cetirizine and loratadine. The developed method was validated in terms of linearity, limits of detection and quantiﬁcation, intra- and inter-day precision, selectivity and robustness.
  
  
  CONCLUSIONS
  Capillary zone electrophoresis proved to be a suitable method for the simulatneous determination of the three studied antihistamine derivatives.
authors: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy, Târgu Mureş, Romania.
authors: Gabriel
authors: G
authors: Hancu
authors: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy, Târgu Mureş, Romania.
authors: Camelia
authors: C
authors: Campian
authors: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy, Târgu Mureş, Romania.
authors: Aura
authors: A
authors: Rusu
authors: Department of Organic Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy, Târgu Mureş, Romania.
authors: Eleonora
authors: E
authors: Mircia
authors: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy, Târgu Mureş, Romania.
authors: Hajnal
authors: H
authors: Kelemen
keywords: Antihistamines; Capillary electrophoresis; Cetirizine; Desloratadine; Loratadine; Separation