title: EndoS reduces the pathogenicity of anti-mCOL7 IgG through reduced binding of immune complexes to neutrophils.
abstract: Endo-β-N-acetylglucosaminidase (EndoS) has been shown to act as a potent pathogen-derived immunomodulatory molecule in autoimmune diseases. Here we investigated how EndoS treatment reduces the pathogenicity of rabbit anti-mCOL7 IgG using different experimental models of epidermolysis bullosa acquisita (EBA). Our results show that the EndoS treatment does not interfere with the binding of the antibody to the antigen but reduces immune complex (IC)-mediated neutrophil activation by impairing the binding of the IC to FcγR on neutrophils. On the basis of this newly identified EndoS-mediated mechanism we hope to develop new strategies in the treatment of the disease.
authors: Priority Area Asthma & Allergy, Research Center Borstel, Airway Research Center North (ARCN), Members of the German Center for Lung Research (DZL), Borstel, Germany ; Laboratory of Autoimmunity, The Medical College of Xiamen University, Xiamen University, Xiamen, China.
authors: Xinhua
authors: X
authors: Yu
authors: Laboratory of Autoimmunity, The Medical College of Xiamen University, Xiamen University, Xiamen, China.
authors: Junfeng
authors: J
authors: Zheng
authors: Division of Infection Medicine, Department of Clinical Science, Lund University, Lund, Sweden.
authors: Mattias
authors: M
authors: Collin
authors: Department of Dermatology, University of Lübeck, Lübeck, Germany.
authors: Enno
authors: E
authors: Schmidt
authors: Department of Dermatology, University of Lübeck, Lübeck, Germany.
authors: Detlef
authors: D
authors: Zillikens
authors: Priority Area Asthma & Allergy, Research Center Borstel, Airway Research Center North (ARCN), Members of the German Center for Lung Research (DZL), Borstel, Germany.
authors: Frank
authors: F
authors: Petersen
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex / Q000276:immunology*; D000941:Antigens / Q000378:metabolism; D024143:Collagen Type VII / Q000276:immunology*; D004195:Disease Models, Animal; D016107:Epidermolysis Bullosa Acquisita / Q000473:pathology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D018375:Neutrophil Activation; D009504:Neutrophils / Q000276:immunology*; D011485:Protein Binding; D011817:Rabbits
chemical_list: D000936:Antigen-Antibody Complex; D000941:Antigens; D024143:Collagen Type VII; D007074:Immunoglobulin G; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase