title: [Human umbilic mesenchymal stromal cells repairs diabetic foot in rats associated with VEGF expressional change].
abstract: OBJECTITVE: To explore the mechanism of human umbilic mesenchymal cells (HUMSCs) implantation for the treatment of diabetic foot in rats associate with vascular endothelia growth factor (VEGF) expression changes.
  
  
  METHODS
  After diabetic foot model in rats were established by administration of streptozotozin (STZ) in intraperitoneal injection (2 weeks), ulceration in foot was induced by incision injury combined with swearing staphylococcus aureas. Then, HUMSCs were smeared on the ulceration of foot in diabetic rats. Ten days later, the densities of blood vessel and the level of VEGF expression were determined by using immunohistochemistry, RT-PCR and Western blot.
  
  
  RESULTS
  HUMSC grafts reduced significantly the volume of ulceration in diabetic foot rats (P < 0.05). RT-PCR and Western blot showed that VEGF and its mRNA were significantly upregulated (P < 0.05). VEGF immunstaining was found in blood vessels and the densities of blood vessels in HUMSC group were increased significantly (P < 0.05).
  
  
  CONCLUSIONS
  HUMSC implantation showed a positive role in promoting the recovery of the ulceration in foot with diabetic rats.
authors: Yan-an Hospital Affiliated to Kunming Medical University, Kunming 650051, China.
authors: Ying
authors: Y
authors: Wang
authors: Institute of Neuroscience, Kunming Medical University, Kunming 650031, China.
authors: Qi-Qin
authors: QQ
authors: Dan
authors: Yan-an Hospital Affiliated to Kunming Medical University, Kunming 650051, China.
authors: Qing-Ping
authors: QP
authors: Wang
authors: Yan-an Hospital Affiliated to Kunming Medical University, Kunming 650051, China.
authors: Ning
authors: N
authors: Zhou
authors: Yan-an Hospital Affiliated to Kunming Medical University, Kunming 650051, China.
authors: Xing-Fang
authors: XF
authors: Jin
authors: Yan-an Hospital Affiliated to Kunming Medical University, Kunming 650051, China.
authors: Zong-Liua
authors: ZL
authors: Hou
authors: Yan-an Hospital Affiliated to Kunming Medical University, Kunming 650051, China.
authors: Bao-Kun
authors: BK
authors: Peng
authors: Institute of Neuroscience, Kunming Medical University, Kunming 650031, China.
authors: Ting-Hua
authors: TH
authors: Wang
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000473:pathology; D017719:Diabetic Foot / Q000628:therapy*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D012333:RNA, Messenger; D051381:Rats; D014470:Umbilical Cord / Q000166:cytology; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*; D014945:Wound Healing
chemical_list: D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat