title: [Genome sequences of coxsackievirus B5 isolated from viral encephalitis patients in Henan province, 2011].
abstract: OBJECTIVE
  To analyze the complete genome sequences of two coxsackievirus B5 (CVB5) isolated in Henan province, 2011.
  
  
  METHODS
  Specimens were collected from viral encephalitis patients and followed by viral isolation on them. RNA were extracted from positive isolates and the amplified products were sequenced. The full-length genomes of them were acquired by assembling the fragments, using DNAStar 5.01 software while phylogenetic analysis were performed with Mega 5.05 and other software.
  
  
  RESULTS
  The genomes RNA of 03001N and 17Y showed 7408 bp and 7404 bp long, and the 5'- and 3'-untranslated regions were 747 bp, 743 bp and 103 bp, 103 bp, respectively. BLAST analysis of these two isolates, based on the complete genome, showed 97% identity, with both of them having the highest similarity(98%, 99%)to the CVB5 strain isolated from Henan in 2010 rather than other CVB5 strains. Coding regions of both isolates were 6558 bp, code for a polyprotein of 2185 amino acids (aa) and both of them showed 99% amino acid identity. Phylogenetic tree in VP1 region showed that the two isolates belonged to the same clade with other strains isolated from all over the country in the past years, except for some CVB5 strains isolated from Henan and Shandong province in 2009 that formed the other cluster.
  
  
  CONCLUSIONS
  It seemed that more than one group of CVB5 were circulating in Henan province and these two isolates appeared the main epidemic strains circulating in the past years.
authors: Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450016, China.
authors: Hong-xia
authors: HX
authors: Ma
authors: Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450016, China.
authors: Jing-jing
authors: JJ
authors: Pan
authors: Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450016, China.
authors: Kai
authors: K
authors: Kang
authors: Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450016, China.
authors: Zhi-qiang
authors: ZQ
authors: Xie
authors: Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450016, China.
authors: Wei-ping
authors: WP
authors: Ru
authors: Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450016, China.
authors: Hao-min
authors: HM
authors: Chen
authors: Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450016, China.
authors: Xue-yong
authors: XY
authors: Huang
authors: Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450016, China. Email: xubl@hncdc.com.cn.
authors: Bian-li
authors: BL
authors: Xu
mesh_terms: D001483:Base Sequence; D002681:China / Q000453:epidemiology; D018792:Encephalitis, Viral / Q000453:epidemiology / Q000821:virology*; D029822:Enterovirus B, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D005814:Genes, Viral; D006801:Humans; D010802:Phylogeny