title: Development of a Specific and Rapid Diagnostic Method for Detecting Influenza A (H1N1) pdm09 Virus Infection Using Immunochromatographic Assay.
abstract: OBJECTIVE
  The aim of this study was to develop an immunochromatographic assay (ICA) for the detection of influenza A (H1N1) pdm09 virus infection.
  
  
  METHODS
  Several monoclonal antibodies against influenza A (H1N1) pdm09 virus were generated and an ICA (pdm09-ICA) was developed for the rapid and specific detection of influenza A (H1N1) pdm09 virus infection. The specificity and sensitivity of the developed assay were compared with that of hemagglutination assay and real-time reverse-transcription polymerase chain reaction (rRT-PCR).
  
  
  RESULTS
  The detection limit was estimated to be 1/2 (8) hemagglutinating unit; the sensitivity and specificity rates of pdm09-ICA were 75.86% (110/145) and 100% (43/43), respectively, compared with rRT-PCR. The cross-reactivity for 20 influenza viruses, including seasonal H1N1 viruses, was found to be negative except for the H1N1 virus (A/Swine/Korea/GC0503/2005).
  
  
  CONCLUSIONS
  These results indicate that the proposed method can be easily used for rapid and specific detection of the pdm09 infection. The assay developed in this study would be a useful tool for distinguishing the pdm09 infection from seasonal influenza A and B infections.
authors: Biotech Laboratory, Standard Diagnostics Inc., Yongin, Korea ; Department of Life Science, College of Natural Science, Kyonggi University, Suwon, Korea.
authors: Mi Jung
authors: MJ
authors: Ji
authors: Biotech Laboratory, Standard Diagnostics Inc., Yongin, Korea.
authors: Byung Ki
authors: BK
authors: Cho
authors: Biotech Laboratory, Standard Diagnostics Inc., Yongin, Korea.
authors: Young Shik
authors: YS
authors: Cho
authors: Department of Laboratory Medicine, College of Medicine, Soonchunhyang University, Cheonan, Korea.
authors: Young Jin
authors: YJ
authors: Choi
authors: Division of Influenza Virus, Korea National Institute of Health, Osong, Korea.
authors: Donghyok
authors: D
authors: Kwon
authors: Division of Influenza Virus, Korea National Institute of Health, Osong, Korea.
authors: Kyeongcheol
authors: K
authors: Shin
authors: Division of Influenza Virus, Korea National Institute of Health, Osong, Korea.
authors: Joo-Yeon
authors: JY
authors: Lee
authors: Division of Influenza Virus, Korea National Institute of Health, Osong, Korea.
authors: Chun
authors: C
authors: Kang
authors: Department of Life Science, College of Natural Science, Kyonggi University, Suwon, Korea.
authors: Byoung Su
authors: BS
authors: Yoon
keywords: immunochromatographic assay; immunochromatography test; influenza; pdm09