title: Pyrosequencing: powerful and quantitative sequencing technology.
abstract: Pyrosequencing is a sequencing-by-synthesis method for DNA analysis that has emerged as a platform not only for de novo sequencing applications, but also for quantitative analysis of genomic methylation, single-nucleotide polymorphisms, and allele quantification. In this unit, we describe a complete workflow from sample to result that is suitable for each of these applications. As cytosine conversion is a key element of successful methylation analysis using pyrosequencing, a support protocol for bisulfite treatment is also included.
authors: Qiagen GmbH, Hilden, Germany.
authors: Martin
authors: M
authors: Kreutz
authors: Qiagen GmbH, Hilden, Germany.
authors: Norbert
authors: N
authors: Hochstein
authors: Qiagen GmbH, Hilden, Germany.
authors: Julia
authors: J
authors: Kaiser
authors: Qiagen GmbH, Hilden, Germany.
authors: Frank
authors: F
authors: Narz
authors: Qiagen GmbH, Hilden, Germany.
authors: Ralf
authors: R
authors: Peist
mesh_terms: D018899:CpG Islands; D003596:Cytosine; D019175:DNA Methylation; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D003596:Cytosine
keywords: AQ; DNA methylation; SNP; genome analysis; pyrosequencing; sequencing