title: Generation of rabbit monoclonal antibodies.
abstract: Rabbit hybridomas are gaining wide acceptance as serologic reagents to identify a variety of antigens, including proteins, small peptides, phosphorylated proteins, and polysaccharides. Rabbits make high-affinity IgG antibodies, all of which bind with high affinity to Protein A from Staphylococcus aureus and Protein G from Group G Streptococcus. Consequently, rabbit monoclonal antibodies of desired specificity can be rapidly detected using Protein A/G as secondary reagents. Here we describe the method for generating rabbit monoclonal antibodies using the rabbit hybridoma fusion partner 240E-1. The method begins with the immunization of rabbits and ends with the cloning the antigen-specific hybridomas.
authors: Stritch School of Medicine, Loyola University Chicago, Maywood, IL, USA.
authors: Pi-Chen
authors: PC
authors: Yam
authors: Katherine L
authors: KL
authors: Knight
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D011817:Rabbits; D013205:Staphylococcal Protein A / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; C034284:IgG Fc-binding protein, Streptococcus; D013205:Staphylococcal Protein A