title: Genotypic and phenotypic comparison of enteroaggregative Escherichia coli isolates from HIV-positive and non-HIV diarrheal samples.
abstract: Enteroaggregative Escherichia coli (EAEC) have been isolated from both HIV-positive and non-HIV diarrheal samples. In this study a collection of 18 isolates from these two groups were compared for biofilm formation and antibiotic resistance and for the presence of 14 virulence-related genes. All the HIV-positive and over 66% of the non- HIV strains were PCR-negative for adhesion-related sequences indicating that as yet unknown adhesins may play a role. However, despite some variations, the prevalence rate of the virulence-related genes was not significantly different in the two groups. HIV-positive isolates were biofilm producer but only a single weak biofilm former was observed among the non-HIV strains. The rate of resistance to most of the antibiotics used was higher among the HIV-positive group than the non-HIV isolates, but was significantly higher for amoxicillin-calvulanic acid (100%) and nalidixic acid (55.5%). Pulse field gel electrophoresis of the isolates produced 17 unique profiles reflecting the exiting heterogeneity of the isolates.
authors: Anis
authors: A
authors: Jafari
authors: Ebrahim
authors: E
authors: Shafaei
authors: Mana
authors: M
authors: Oloomi
authors: Mohammad-Reza
authors: MR
authors: Aghasadeghi
authors: Molecular Biology Department, Pasteur Institute of Iran, Tehran, Iran. saeidbouzari@yahoo.com.
authors: Saeid
authors: S
authors: Bouzari
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018441:Biofilms / Q000254:growth & development; D003967:Diarrhea / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial; D004926:Escherichia coli / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005838:Genotype; D006679:HIV Seropositivity / Q000382:microbiology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D010641:Phenotype
chemical_list: D000900:Anti-Bacterial Agents