title: Construction of reductive pathway in Saccharomyces cerevisiae for effective succinic acid fermentation at low pH value.
abstract: Succinic acid is an important precursor for the synthesis of high-value-added products. Saccharomyces cerevisiae is a suitable platform for succinic acid production because of its high tolerance towards acidity. In this study, a modified pathway for succinate production was established and investigated in S. cerevisiae. The engineered strain could produce up to 6.17±0.34g/L of succinate through the constructed pathway. The succinate titer was further improved to 8.09±0.28g/L by the deletion of GPD1 and even higher to 9.98±0.23g/L with a yield of 0.32mol/mol glucose through regulation of biotin and urea levels. Under optimal supplemental CO2 conditions in a bioreactor, the engineered strain produced 12.97±0.42g/L succinate with a yield of 0.21mol/mol glucose at pH 3.8. These results demonstrated that the proposed engineering strategy was efficient for succinic acid production at low pH value.
authors: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors: Daojiang
authors: D
authors: Yan
authors: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors: Caixia
authors: C
authors: Wang
authors: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors: Jiemin
authors: J
authors: Zhou
authors: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors: Yilan
authors: Y
authors: Liu
authors: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100190, PR China.
authors: Maohua
authors: M
authors: Yang
authors: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100190, PR China. Electronic address: jmxing@home.ipe.ac.cn.
authors: Jianmin
authors: J
authors: Xing
mesh_terms: D019149:Bioreactors / Q000382:microbiology; D001710:Biotin / Q000494:pharmacology; D002119:Calcium Carbonate / Q000494:pharmacology; D057075:Enzyme Assays; D005285:Fermentation / Q000187:drug effects; D017353:Gene Deletion; D005947:Glucose / Q000378:metabolism; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+) / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D060847:Metabolic Engineering / Q000379:methods*; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D010084:Oxidation-Reduction / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D019802:Succinic Acid / Q000378:metabolism*; D014508:Urea / Q000494:pharmacology
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D001710:Biotin; D014508:Urea; D019802:Succinic Acid; C568531:GPD1 protein, S cerevisiae; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+); D002119:Calcium Carbonate; D005947:Glucose
keywords: Low pH; Reducing power; Reductive pathway; Saccharomyces cerevisiae; Succinic acid