title: Effects of ECM protein mimetics on adhesion and proliferation of chorion derived mesenchymal stem cells.
abstract: BACKGROUND
  We evaluated the effects of fibronectin, collagen, cadherin, and laminin based extracellular matrix (ECM) protein mimetics coated with mussel derived adhesive protein (MAP) on adhesion and proliferation of chorionic mesenchymal stem cells (cMSCs).
  
  
  METHODS
  Human placental chorionic tissues from term third-trimester pregnancies (n=3) were used. The cMSCs were cultured on rationally designed ECM protein mimetics coated with MAP on plastic surfaces with the addition of reduced fetal bovine serum (0.5%, 1% FBS). Adhesion capabilities were monitored by a real time cell analysis system (RTCA) utilizing an impedance method. Proliferation capabilities were monitored by RTCA and MTS assay.
  
  
  RESULTS
  Of the ECM protein mimetics tested, GRGDSP(FN) coated surfaces exhibited the highest adhesion and proliferation capabilities on RTCA at FBS concentration of 0.5% and 1%. When 0.5% FBS was added to ECM protein mimetics during the MTS assay, GRGDSP(FN), REDV(FN), and collagen mimetics, GPKGAAGEPGKP(ColI) showed higher cMSCs proliferation compared with the control. When 1% FBS was added, GRGDSP(FN) and TAIPSCPEGTVPLYS(ColIV) showed significant cMSCs proliferation capacity.
  
  
  CONCLUSIONS
  Fibronectin mimetics, GRGDSP(FN) amino acid sequence showed the highest adhesion and proliferation capabilities. In addition, results from RTCA assessment of cell viability correlated well with the tetrazolium-based MTS assay.
authors: 1. Department of Laboratory Medicine College of Medicine, The Catholic University of Korea, Seoul, South Korea; ; 3. Department of Biomedical Science, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
authors: Ji-Hyun
authors: JH
authors: Kim
authors: 1. Department of Laboratory Medicine College of Medicine, The Catholic University of Korea, Seoul, South Korea;
authors: Dong Wook
authors: DW
authors: Jekarl
authors: 1. Department of Laboratory Medicine College of Medicine, The Catholic University of Korea, Seoul, South Korea;
authors: Myungshin
authors: M
authors: Kim
authors: 1. Department of Laboratory Medicine College of Medicine, The Catholic University of Korea, Seoul, South Korea;
authors: Eun-Jee
authors: EJ
authors: Oh
authors: 1. Department of Laboratory Medicine College of Medicine, The Catholic University of Korea, Seoul, South Korea;
authors: Yonggoo
authors: Y
authors: Kim
authors: 2. Department of Obstetrics and Gynecology, College of Medicine, The Catholic University of Korea, Seoul, South Korea;
authors: In Yang
authors: IY
authors: Park
authors: 2. Department of Obstetrics and Gynecology, College of Medicine, The Catholic University of Korea, Seoul, South Korea;
authors: Jong Chul
authors: JC
authors: Shin
mesh_terms: D000818:Animals; D032701:Biomimetics; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002823:Chorion / Q000166:cytology; D005109:Extracellular Matrix / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000008:administration & dosage*; D005260:Female; D005353:Fibronectins / Q000008:administration & dosage*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D011247:Pregnancy
chemical_list: D016326:Extracellular Matrix Proteins; D005353:Fibronectins
keywords: cell proliferation assay.; chorionic mesenchymal stem cell; extracellular matrix; protein mimetics; real time cell analysis