title: Study of the variations in apoptotic factors in hippocampus of male rats with posttraumatic stress disorder.
abstract: BACKGROUND
  Post-traumatic stress disorder (PTSD) is a stress-related psychosomatic disorder caused by occurrence of a traumatic event and the hippocampus volume of the patients with Post-traumatic stress disorder decreased. However, the mechanisms that cause such damage are not well-understood. The aim of this study is to detect the expression of apoptosis-related Bax, Bcl-2, Caspase-3 and Insulin-like growth Factor-I proteins in the hippocampus region in the Predatory stress rats.
  
  
  METHODS
  A total of 70 male wistar rats were divided into Predatory stress groups of 1d, 2d, 3d, 7d, 14d, 30d and a normal control group (N = 10). Rats were subjected to 5 min of predatory stress and then exposed to the elevated plus-maze (EPM). Serum corticosterone and Insulin-like growth factor-1 level of Hippocampus were measured by ELISA technique. The expression of Bax, Bcl-2, and Caspase-3 were detected by western blotting.
  
  
  RESULTS
  Rats spent significantly more time in closed arms of the elevated plus maze (EPM) than control group after exposure to stress. Serum levels of corticosterone significantly increased at 2d-3d. The expression of hippocampal IGF-1 was significantly up-regulated at 1d-2d after stress. Both Bax and the ratio of Bax/Bcl-2 significantly peaked at Predatory stress 2d-14d. Caspase3 was significantly active among 2d-30 compared to the normal control.
  
  
  CONCLUSIONS
  The activation of caspase-3 in the stress groups indicates that apoptosis may be one of the reasons inducing hippocampus atrophy and play roles in the pathogenesis of PTSD. Increase in hippocampus levels of IGF-1 during early PTSD might be involved in the early molecular inhibitory mechanism of apoptosis in PTSD.
authors: Department of Anatomical Science and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Tehran, Iran. ; Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Tehran, Iran. ; Neuroscience Research Center, Shahid Beheshi University of Medical Sciences, Tehran, Iran.
authors: Behrang
authors: B
authors: Alani
authors: Neuroscience Research Center, Shahid Beheshi University of Medical Sciences, Tehran, Iran.
authors: Nader
authors: N
authors: Maghsoudi
authors: Research Group on Health Psychology, University of Leuven, Belgium, Iran.
authors: Ali
authors: A
authors: Khatibi
authors: Department of Physiology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran. ; Physiology Research Center, Kashan University of Medical Sciences, Kashan, Iran.
authors: Mahdi
authors: M
authors: Noureddini
authors: Neuroscience Research Center, Shahid Beheshi University of Medical Sciences, Tehran, Iran.
authors: Farzad
authors: F
authors: Asefifar
authors: Neuroscience Research Center, Shahid Beheshi University of Medical Sciences, Tehran, Iran. ; Behavioural Science Research Center, Department of Psychiatry, Imam Hossein Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors: Jamal
authors: J
authors: Shams
keywords: Apoptosis; Bax; Bcl2; caspase-3; corticosterone; insulin-like growth factor-1; post-traumatic stress disorder; predator stress