title: Fluorescent Random Amplified Microsatellites (F-RAMS) analysis of mushrooms as a forensic investigative tool.
abstract: The capability of Fluorescent Random Amplified Microsatellites (F-RAMS) to profile hallucinogenic mushrooms to species and sub-species level was assessed. Fifteen samples of Amanita rubescens and 22 samples of other hallucinogenic and non-hallucinogenic mushrooms of the genera Amanita and Psilocybe were profiled using two fluorescently-labeled, 5'degenerate primers, 5'-6FAM-SpC3-DD (CCA)5 and 5'-6FAM-SpC3-DHB (CGA)5, which target different microsatellite repeat regions. Among the two primers, 5'-6FAM-SpC3-DHB (CGA)5 provided more reliable data for identification purposes, by grouping samples of the same species and clustering closely related species together in a dendrogram based on amplicon similarities. A high degree of intra-specific variation between the 15 A. rubescens samples was shown with both primers and the amplicons generated for all A. rubescens samples were organized into three classes of amplicons (discriminant, private, and marker) based on their individualizing potential.
authors: Department of Chemistry and Biochemistry, International Forensic Research Institute, Florida International University, 11200 SW 8th Street, Miami, FL 33199, United States.
authors: Beatrice
authors: B
authors: Kallifatidis
authors: Nuclear Physics Institute, v.v.i., Academy of Sciences of the Czech Republic, Řež 130, CZ-25068 Řež near Prague, Czech Republic.
authors: Jan
authors: J
authors: Borovička
authors: Institute of Molecular and Translational Medicine of the Faculty of Medicine and Dentistry of Palacký University in Olomouc, Hněvotínská 5, CZ-77900 Olomouc, Czech Republic.
authors: Jana
authors: J
authors: Stránská
authors: Institute of Molecular and Translational Medicine of the Faculty of Medicine and Dentistry of Palacký University in Olomouc, Hněvotínská 5, CZ-77900 Olomouc, Czech Republic.
authors: Jiří
authors: J
authors: Drábek
authors: Department of Chemistry and Biochemistry, International Forensic Research Institute, Florida International University, 11200 SW 8th Street, Miami, FL 33199, United States; Department of Biological Sciences, Florida International University, 11200 SW 8th Street, Miami, FL 33199, United States. Electronic address: millsd@fiu.edu.
authors: Deetta K
authors: DK
authors: Mills
mesh_terms: D000545:Amanita / Q000235:genetics*; D016172:DNA Fingerprinting / Q000379:methods; D017931:DNA Primers; D005453:Fluorescence; D005456:Fluorescent Dyes; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D055391:Psilocybe / Q000235:genetics*
chemical_list: D017931:DNA Primers; D005456:Fluorescent Dyes
keywords: DNA profiling; Hallucinogenic mushrooms; Random Amplified Microsatellites