title: Temporal gene expression profile after acute electroconvulsive stimulation in the rat.
abstract: Electroconvulsive therapy (ECT) remains one of the most effective treatments of major depression. It has been suggested that the mechanisms of action involve gene expression. In recent decades there have been several investigations of gene expression following both acute and chronic electroconvulsive stimulation (ECS). These studies have focused on several distinct gene targets but have generally included only few time points after ECS for measuring gene expression. Here we measured gene expression of three types of genes: Immediate early genes, synaptic proteins, and neuropeptides at six time points following an acute ECS. We find significant increases for c-Fos, Egr1, Neuritin 1 (Nrn 1), Bdnf, Snap29, Synaptotagmin III (Syt 3), Synapsin I (Syn 1), and Psd95 at differing time points after ECS. For some genes these changes are prolonged whereas for others they are transient. Npy expression significantly increases whereas the gene expression of its receptors Npy1r, Npy2r, and Npy5r initially decreases. These decreases are followed by a significant increase for Npy2r, suggesting anticonvulsive adaptations following seizures. In summary, we find distinct changes in mRNA quantities that are characteristic for each gene. Considering the observed transitory and inverse changes in expression patterns, these data underline the importance of conducting measurements at several time points post-ECS.
authors: Laboratory of Neurobiology, Department of Health Science and Technology, Aalborg University, Aalborg, Denmark.
authors: Mads
authors: M
authors: Dyrvig
authors: Laboratory of Neural Plasticity, Department of Neuroscience and Pharmacology, University of Copenhagen, Copenhagen, Denmark.
authors: Søren H
authors: SH
authors: Christiansen
authors: Laboratory of Neural Plasticity, Department of Neuroscience and Pharmacology, University of Copenhagen, Copenhagen, Denmark.
authors: David P D
authors: DP
authors: Woldbye
authors: Laboratory of Neurobiology, Department of Health Science and Technology, Aalborg University, Aalborg, Denmark. Electronic address: jlichota@hst.aau.dk.
authors: Jacek
authors: J
authors: Lichota
mesh_terms: D000818:Animals; D004565:Electroconvulsive Therapy / Q000009:adverse effects*; D020869:Gene Expression Profiling; D017781:Genes, Immediate-Early / Q000235:genetics*; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D023421:Models, Animal; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D018013:Receptors, Neuropeptide / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D017476:Receptors, Neuropeptide Y / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D012640:Seizures / Q000235:genetics; D013570:Synaptic Membranes / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome
chemical_list: C066211:Npy1r protein, rat; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; D018013:Receptors, Neuropeptide; D017476:Receptors, Neuropeptide Y; C095889:neuropeptide Y2 receptor; C100568:neuropeptide Y5 receptor
keywords: Depression; Hippocampus; Immediate early genes; Neuropeptides; Synaptic vesicle proteins; qPCR