title: Genomic Reprogramming and Skin-Like Maturation of Engineered Human Skin Substitutes.
abstract: BACKGROUND
  Cultured skin substitutes (CSS) have been evaluated in clinical trials as an adjunctive treatment for large full-thickness burn wounds. Prepared with autologous fibroblasts, keratinocytes, and biopolymers, CSS can provide permanent wound closure upon engraftment to excised burns.
  
  
  OBJECTIVE
  CSS containing only two cell types are limited in anatomy and physiology compared with normal uninjured skin. Identifying deficiencies in CSS can instruct further tissue engineering advances.
  Expression profiling of CSS duringin vitromaturation and after transplantationin vivowith Affymetrix GeneChip®Arrays was used to characterize pathways that are abnormal or deficient in CSS compared with normal human skin. Examination of the large data set generated from microarray expression analysis revealed similarities between healed CSS and normal skin, particularly in expression of genes involved in epidermal differentiation and barrier function. However, deficiencies in several pathways were also noted, such as the genetic pathways regulating development of adnexal structures, including hair follicles.
  A deeper understanding of the cellular and molecular events guiding morphogenesis of engineered skin can lead to improvements that will increase clinical efficacy.
  
  
  CONCLUSIONS
  The results of GeneChip analysis highlighted the processes that act to regulate tissue developmentin vitroand adaptation to the wound environment and healingin vivo. This knowledge can be used to inform modifications to the model that will facilitate incorporation of additional cell types for increased homology with native human skin and improved functional outcome for burn patients treated with engineered skin grafts.
authors: Research Department, Shriners Hospitals for Children-Cincinnati , Cincinnati, Ohio. ; Department of Surgery, University of Cincinnati College of Medicine , Cincinnati, Ohio.
authors: Dorothy M
authors: DM
authors: Supp