title: Viability of talus osteochondral defect cartilage for chondrocyte harvesting: results of 151 patients.
abstract: BACKGROUND
  Large talar cartilage defects can be treated with either autologous chondrocyte implantation or matrix autologous chondrocyte implantation. Both techniques depend on successful harvesting of the chondrocytes. In the past, they have come from the ipsilateral knee, which has been associated with donor site morbidity. We hypothesized that damaged cartilage from the talus can be used as a reliable source for chondrocyte cell harvesting in preparation for possible matrix-induced autologous chondrocyte implantation (MACI).
  
  
  METHODS
  Chondrocytes were harvested from the injured talar cartilage during ankle arthroscopy and sent to a cell laboratory, measured for initial biopsy weight, cultured for 4 to 6 weeks, and then analyzed for viability. A total of 151 patients were analyzed.
  
  
  RESULTS
  The average biopsy initial weight was 187.1 mg. The average number of cells was 3.13 × 10(5). The viability of the chondrocytes provided by the manufacturer averaged 92.3% (range, 33%-100%).
  
  
  CONCLUSIONS
  Chondrocytes harvested from the damaged talar articular cartilage were functional and proliferated with an average viability of 92%.
  
  
  CONCLUSIONS
  This technique may provide a useful source of chondrocytes if needed for a future cell-based regenerative procedure such as MACI while eliminating the need to harvest chondrocytes from the knee or other intact areas of cartilage on the talus.
  
  
  METHODS
  Level IV, case series.
authors: Sutter Medical Group-Auburn, Auburn, California.
authors: Christopher
authors: C
authors: Kreulen
authors: Eric
authors: E
authors: Giza
authors: Jason
authors: J
authors: Kim
authors: Valentina
authors: V
authors: Campanelli
authors: Martin
authors: M
authors: Sullivan
mesh_terms: D000328:Adult; D001182:Arthroscopy; D001706:Biopsy; D002358:Cartilage, Articular / Q000293:injuries / Q000601:surgery*; D018929:Cell Culture Techniques; D019902:Chondrocytes / Q000637:transplantation*; D005260:Female; D006801:Humans; D008297:Male; D013628:Talus / Q000293:injuries / Q000601:surgery*; D020858:Tissue and Organ Harvesting / Q000379:methods*; D014182:Transplantation, Autologous
keywords: matrix-induced autologous chondrocyte implantation (MACI); osteochondral defect; talar chondrocytes; talus