title: Human papillomavirus detection in cervical scrapes from women attended in the Family Health Program.
abstract: OBJECTIVE
  to survey the prevalence of human papillomavirus, associated risk factors and genotype distribution in women who were referred to cervical cancer screening when attended in a Family Health Program.
  
  
  METHODS
  we conducted a cross-sectional survey, investigating 351 women. Polymerase chain reaction for DNA amplification and restriction fragment length polymorphism analysis were used to detect and typify the papillomavirus.
  
  
  RESULTS
  virus infection was detected in 8.8% of the samples. Among the 21 different genotypes identified in this study, 14 were high risk for cervical cancer, and the type 16 was the most prevalent type. The infection was associated with women who had non-stable sexual partners. Low risk types were associated with younger women, while the high risk group was linked to altered cytology.
  
  
  CONCLUSIONS
  in this sample attended a Family Health Program, we found a low rate of papillomavirus infection. Virus frequency was associated to sexual behavior. However, the broad range of genotypes detected deserves attention regarding the vaccine coverage, which includes only HPV prevalent types.
authors.affiliation: Secretaria de Estado de Saúde e Defesa Civil do Estado do Rio de Janeiro, Hospital Estadual Alberto Torres, Rio de JaneiroRJ, Brazil, MSc, RN, Hospital Estadual Alberto Torres, Secretaria de Estado de Saúde e Defesa Civil do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Everton Faccini
authors.initials: EF
authors.lastname: Augusto
authors.affiliation: Universidade Federal Fluminense, NiteróiRJ, Brasil, Doctoral student, Universidade Federal Fluminense, Niterói, RJ, Brasil.
authors.forename: Larissa Silva dos
authors.initials: LS
authors.lastname: Santos
authors.affiliation: Universidade Federal Fluminense, Departamento de Microbiologia e Parasitologia, NiteróiRJ, Brazil, PhD, Full Professor, Departamento de Microbiologia e Parasitologia, Universidade Federal Fluminense, Niterói, RJ, Brazil.
authors.forename: Ledy do Horto dos Santos
authors.initials: Ldo H
authors.lastname: Oliveira
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002584:Cervix Uteri / Q000821:virology*; D003430:Cross-Sectional Studies; D005192:Family Health; D005260:Female; D006801:Humans; D008875:Middle Aged; D065006:Papanicolaou Test; D027383:Papillomaviridae / Q000145:classification / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000821:virology*; D015995:Prevalence; D014626:Vaginal Smears; D055815:Young Adult