title: Paeoniflorin inhibits the maturation and immunostimulatory function of allergen-induced murine dendritic cells.
abstract: Dendritic cells (DCs) as the front lines of defense play a crucial role in allergic contact dermatitis (ACD). Paeoniflorin (PF) has been clinically proven to be effective in the treatment of inflammatory skin diseases such as ACD. However, the mechanisms underlying the anti-inflammatory effect of PF remain unclear. The aim of this study was to explore the effect of PF on the maturation and immunostimulatory function of DCs in the murine model of ACD in vitro. Murine bone marrow-derived DCs were stimulated with the contact sensitizer 1-chloro-2, 4-dinitrobenze (DNCB) in vitro. Surface antigen expression of DCs (MHC II, CD40, CD80, and CD86), as an indicator of maturation DCs and cytokines (IL-12, IFN-γ, IL-10, and TGF-β) after DNCB stimulation in the absence or presence of PF at different doses, was detected. Then, we detected that PF-treated DCs stimulated T cells in response to DNCB. PF inhibited the up-regulation of MHC II, CD80, CD86, and CD40, decreased IL-12p70 secretion, while increased the production of IL-10 and TGF-β, and had no effect on IFN-γ cytokine production by murine bone marrow-derived DCs in response to DNCB. DCs exposed to PF had diminished capacity to stimulate allogeneic T cell proliferation and to activate IFN-γ-producing CD4⁺ T cells and induced CD4⁺CD25⁺Foxp3⁺ T cells and IL-10-producing T cell expansion from naïve CD4⁺ T cells. These results indicate that PF may be effective in preventing and treating ACD in vitro and other inflammatory responses possibly through inhibiting maturation of DCs and limiting their capacity to stimulate T cell responses.
authors.affiliation: Department of Dermatology, Jining First People's Hospital, Shandong, PR China; Department of Mycology, Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing, Jiangsu, PR China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Shi
authors.affiliation: Department of Radiology, Jining First People's Hospital, Shandong, PR China.
authors.forename: Aidong
authors.initials: A
authors.lastname: Ma
authors.affiliation: Department of Dermatology, Jining First People's Hospital, Shandong, PR China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Zheng
authors.affiliation: School of Stomatology, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, PR China.
authors.forename: Guang
authors.initials: G
authors.lastname: Huo
authors.affiliation: Department of Dermatology, Jining First People's Hospital, Shandong, PR China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Yan
authors.affiliation: Department of Dermatology, Jining First People's Hospital, Shandong, PR China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Fu
authors.affiliation: Department of Dermatology, Jining First People's Hospital, Shandong, PR China. Electronic address: qiou_ying@sohu.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: Department of Mycology, Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing, Jiangsu, PR China. Electronic address: liumyco@hotmail.com.
authors.forename: Weida
authors.initials: W
authors.lastname: Liu
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology; D003713:Dendritic Cells / Q000187:drug effects* / Q000502:physiology; D005260:Female; D005960:Glucosides / Q000494:pharmacology*; D007155:Immunologic Factors / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D039821:Monoterpenes / Q000494:pharmacology*; D010587:Phagocytosis / Q000187:drug effects; D006027:Glycosides
chemical_list: D000485:Allergens; D016207:Cytokines; D005960:Glucosides; D007155:Immunologic Factors; D039821:Monoterpenes; C015423:peoniflorin; D006027:Glycosides
keywords: 1-Chloro-2, 4-dinitrobenze; Allergic contact dermatitis; Dendritic cells; Paeoniflorin; Regulatory T cells