title: Inner retinal oxygen delivery and metabolism in streptozotocin diabetic rats.
abstract: OBJECTIVE
  The purpose of the study is to report global measurements of inner retinal oxygen delivery (DO2_IR) and oxygen metabolism (MO2_IR) in streptozotocin (STZ) diabetic rats.
  
  
  METHODS
  Phosphorescence lifetime and blood flow imaging were performed in rats 4 (STZ/4 wk; n = 10) and 6 (STZ/6 wk; n = 10) weeks following injection of STZ to measure retinal arterial (O2A) and venous (O2V) oxygen contents and total retinal blood flow (F). DO2_IR and MO2_IR were calculated from measurements of F and O2A and of F and the arteriovenous oxygen content difference, respectively. Data in STZ rats were compared to those in healthy control rats (n = 10).
  
  
  RESULTS
  Measurements of O2A and O2V were not significantly different among STZ/4 wk, STZ/6 wk, and control rats (P ≥ 0.28). Likewise, F was similar among all groups of rats (P = 0.81). DO2_IR measurements were 941 ± 231, 956 ± 232, and 973 ± 243 nL O2/min in control, STZ/4 wk, and STZ/6 wk rats, respectively (P = 0.95). MO2_IR measurements were 516 ± 175, 444 ± 103, and 496 ± 84 nL O2/min in control, STZ/4 wk, and STZ/6 wk rats, respectively (P = 0.37).
  
  
  CONCLUSIONS
  Global inner retinal oxygen delivery and metabolism were not significantly impaired in STZ rats in early diabetes.
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois.
authors.forename: Justin
authors.initials: J
authors.lastname: Wanek
authors.forename: Pang-Yu
authors.initials: PY
authors.lastname: Teng
authors.forename: Norman P
authors.initials: NP
authors.lastname: Blair
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Shahidi
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000378:metabolism*; D003930:Diabetic Retinopathy / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D020318:Rats, Long-Evans; D012039:Regional Blood Flow; D012160:Retina / Q000378:metabolism* / Q000503:physiopathology
chemical_list: D010100:Oxygen
keywords: delivery; diabetes; metabolism; oxygen; rat; retina