title: Lensless phase contrast microscopy based on multiwavelength Fresnel diffraction.
abstract: We demonstrate a compact, wide-field, quantitative phase contrast microscope that does not require lenses for image formation. High-resolution images are retrieved from Fresnel diffraction patterns recorded at multiple wavelengths, combined with a robust iterative phase retrieval algorithm. Quantitative phase contrast images of living cultured neurons are obtained with a transverse resolution of <2 μm. Our system is well suited for high-resolution live cell imaging and provides a compact, cost-effective alternative to full-sized phase-contrast microscopes.
authors.forename: Daniel W E
authors.initials: DW
authors.lastname: Noom
authors.forename: Kjeld S E
authors.initials: KS
authors.lastname: Eikema
authors.forename: Stefan
authors.initials: S
authors.lastname: Witte