title: QTL detection for coccidiosis (Eimeria tenella) resistance in a Fayoumi × Leghorn F₂ cross, using a medium-density SNP panel.
abstract: BACKGROUND
  Coccidiosis is a major parasitic disease that causes huge economic losses to the poultry industry. Its pathogenicity leads to depression of body weight gain, lesions and, in the most serious cases, death in affected animals. Genetic variability for resistance to coccidiosis in the chicken has been demonstrated and if this natural resistance could be exploited, it would reduce the costs of the disease. Previously, a design to characterize the genetic regulation of Eimeria tenella resistance was set up in a Fayoumi × Leghorn F2 cross. The 860 F2 animals of this design were phenotyped for weight gain, plasma coloration, hematocrit level, intestinal lesion score and body temperature. In the work reported here, the 860 animals were genotyped for a panel of 1393 (157 microsatellites and 1236 single nucleotide polymorphism (SNP) markers that cover the sequenced genome (i.e. the 28 first autosomes and the Z chromosome). In addition, with the aim of finding an index capable of explaining a large amount of the variance associated with resistance to coccidiosis, a composite factor was derived by combining the variables of all these traits in a single variable. QTL detection was performed by linkage analysis using GridQTL and QTLMap. Single and multi-QTL models were applied.
  
  
  RESULTS
  Thirty-one QTL were identified i.e. 27 with the single-QTL model and four with the multi-QTL model and the average confidence interval was 5.9 cM. Only a few QTL were common with the previous study that used the same design but focused on the 260 more extreme animals that were genotyped with the 157 microsatellites only. Major differences were also found between results obtained with QTLMap and GridQTL.
  
  
  CONCLUSIONS
  The medium-density SNP panel made it possible to genotype new regions of the chicken genome (including micro-chromosomes) that were involved in the genetic control of the traits investigated. This study also highlights the strong variations in QTL detection between different models and marker densities.
authors.forename: Nicola
authors.initials: N
authors.lastname: Bacciu
authors.forename: Bertrand
authors.initials: B
authors.lastname: Bed'Hom
authors.forename: Olivier
authors.initials: O
authors.lastname: Filangi
authors.forename: Hélène
authors.initials: H
authors.lastname: Romé
authors.forename: David
authors.initials: D
authors.lastname: Gourichon
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Répérant
authors.forename: Pascale
authors.initials: P
authors.lastname: Le Roy
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Pinard-van der Laan
authors.affiliation: INRA, UMR 1348 PEGASE, Domaine de la Prise, F-35590 Saint-Gilles, France. Olivier.Demeure@rennes.inra.fr.
authors.forename: Olivier
authors.initials: O
authors.lastname: Demeure
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics* / Q000469:parasitology*; D003048:Coccidiosis / Q000235:genetics / Q000662:veterinary*; D003433:Crosses, Genetic; D016786:Eimeria tenella / Q000302:isolation & purification*; D014644:Genetic Variation; D005838:Genotype; D020641:Polymorphism, Single Nucleotide; D011201:Poultry Diseases / Q000235:genetics* / Q000469:parasitology*; D040641:Quantitative Trait Loci