title: CTCF-mediated reduction of vigilin binding affects the binding of HP1α to the satellite 2 locus.
abstract: CCCTC-binding factor (CTCF) has been implicated in numerous aspects of chromosome biology, and vigilin, a multi-KH-domain protein, participates in heterochromatin formation and chromosome segregation. We previously showed that CTCF interacts with vigilin. Here, we show that human vigilin, but not CTCF, colocalizes with HP1α on heterochromatic satellite 2 and β-satellite repeats. CTCF up-regulates the transcription of satellite 2, while vigilin down-regulates it. Vigilin depletion or CTCF overexpression reduces the binding of HP1α on the satellite 2 locus. Furthermore, overexpression of CTCF resists the loading of vigilin onto the satellite 2 locus. Thus CTCF may regulate vigilin behavior and thus indirectly influence the binding of HP1α to the satellite 2 locus.
authors.affiliation: Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Wen-Yan
authors.initials: WY
authors.lastname: Shen
authors.affiliation: Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Qiu-Ying
authors.initials: QY
authors.lastname: Liu
authors.affiliation: Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wei
authors.affiliation: Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Xiao-Qin
authors.initials: XQ
authors.lastname: Yu
authors.affiliation: Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Wen-Li
authors.initials: WL
authors.lastname: Yang
authors.affiliation: Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Xie
authors.affiliation: Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Wen-Quan
authors.initials: WQ
authors.lastname: Liu
authors.affiliation: Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China; Sichuan University "985 project - Science and technology innovation platform for novel drug development", Chengdu 610041, China. Electronic address: qin_1@sina.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Qin
mesh_terms: D000076246:CCCTC-Binding Factor; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D004276:DNA, Satellite / Q000378:metabolism*; D057809:HEK293 Cells; D006367:HeLa Cells; D006570:Heterochromatin / Q000378:metabolism; D006801:Humans; D061986:MCF-7 Cells; D011485:Protein Binding; D021381:Protein Transport; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012097:Repressor Proteins / Q000502:physiology*; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: C000715594:CBX5 protein, human; D000076246:CCCTC-Binding Factor; C000619697:CTCF protein, human; D002868:Chromosomal Proteins, Non-Histone; D004276:DNA, Satellite; D006570:Heterochromatin; D016601:RNA-Binding Proteins; D012097:Repressor Proteins; D000090266:Chromobox Protein Homolog 5; C075197:high density lipoprotein binding protein
keywords: CCCTC-binding factor; HP1α; Satellite 2; Vigilin