title: Design of a comprehensive biochemistry and molecular biology experiment: phase variation caused by recombinational regulation of bacterial gene expression.
abstract: Scientific experiments are indispensable parts of Biochemistry and Molecular Biology. In this study, a comprehensive Biochemistry and Molecular Biology experiment about Salmonella enterica serovar Typhi Flagellar phase variation has been designed. It consisted of three parts, namely, inducement of bacterial Flagellar phase variation, antibody agglutination test, and PCR analysis. Phase variation was observed by baterial motility assay and identified by antibody agglutination test and PCR analysis. This comprehensive experiment can be performed to help students improve their ability to use the knowledge acquired in Biochemistry and Molecular Biology.
authors.affiliation: Department of Biochemistry and Molecular Biology, Jiangsu University School of Medical Technology, Zhenjiang, Jiangsu, 212013, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Sheng
authors.forename: Shungao
authors.initials: S
authors.lastname: Xu
authors.forename: Renyun
authors.initials: R
authors.lastname: Lu
authors.forename: Dadzie
authors.initials: D
authors.lastname: Isaac
authors.forename: Xueyi
authors.initials: X
authors.lastname: Zhang
authors.forename: Haifang
authors.initials: H
authors.lastname: Zhang
authors.forename: Huifang
authors.initials: H
authors.lastname: Wang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Qiao
authors.forename: Xinxiang
authors.initials: X
authors.lastname: Huang
mesh_terms: D001671:Biochemistry / Q000379:methods*; D015964:Gene Expression Regulation, Bacterial; D008967:Molecular Biology / Q000379:methods*; D011995:Recombination, Genetic
keywords: Salmonella enterica serovar Typhi; comprehensive experiment; phase variation; recombinational regulation of gene expression