title: Trichomonas vaginalis metronidazole resistance is associated with single nucleotide polymorphisms in the nitroreductase genes ntr4Tv and ntr6Tv.
abstract: Metronidazole resistance in the sexually transmitted parasite Trichomonas vaginalis is a problematic public health issue. We have identified single nucleotide polymorphisms (SNPs) in two nitroreductase genes (ntr4Tv and ntr6Tv) associated with resistance. These SNPs were associated with one of two distinct T. vaginalis populations identified by multilocus sequence typing, yet one SNP (ntr6Tv A238T), which results in a premature stop codon, was associated with resistance independent of population structure and may be of diagnostic value.
authors.affiliation: Femeris Women's Health Research Center, Medical Diagnostic Laboratories, Hamilton, New Jersey, USA.
authors.forename: Teresa E
authors.initials: TE
authors.lastname: Paulish-Miller
authors.forename: Peter
authors.initials: P
authors.lastname: Augostini
authors.forename: Jessica A
authors.initials: JA
authors.lastname: Schuyler
authors.forename: William L
authors.initials: WL
authors.lastname: Smith
authors.forename: Eli
authors.initials: E
authors.lastname: Mordechai
authors.forename: Martin E
authors.initials: ME
authors.lastname: Adelson
authors.forename: Scott E
authors.initials: SE
authors.lastname: Gygax
authors.forename: William E
authors.initials: WE
authors.lastname: Secor
authors.forename: David W
authors.initials: DW
authors.lastname: Hilbert
mesh_terms: D000981:Antiprotozoal Agents / Q000494:pharmacology; D018388:Codon, Terminator / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics; D008795:Metronidazole / Q000494:pharmacology*; D009601:Nitroreductases / Q000235:genetics*; D021261:Parasitic Sensitivity Tests; D020641:Polymorphism, Single Nucleotide; D015800:Protozoan Proteins / Q000235:genetics*; D014246:Trichomonas vaginalis / Q000187:drug effects* / Q000235:genetics*
chemical_list: D000981:Antiprotozoal Agents; D018388:Codon, Terminator; D015800:Protozoan Proteins; D008795:Metronidazole; D009601:Nitroreductases