title: Brachybacterium huguangmaarense sp. nov., isolated from Lake sediment.
abstract: A Gram-stain positive strain, M1T, was isolated from the sediment of Maar Lake in Zhanjiang, Guangdong Province, China. The diagnostic cell-wall diamino acid was meso-diaminopimelic acid, and mycolic acids were not detected. The polar lipid profile of strain M1T consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unknown glycolipid. The predominant quinone was MK-7, with MK-6 as a minor component. The major fatty acids were anteiso-C15:0 and anteiso-C17:0, with iso-C18:0 as a minor component. The DNA G+C content of the genomic DNA was 71.0 mol%. 16S rRNA gene sequence analysis showed that strain M1T belongs to the family Dermabacteraceae, sharing highest sequence similarity with Brachybacterium nesterenkovii JCM 11648T (98.1%). Furthermore, a combination of DNA-DNA relatedness and physiological and biochemical properties indicated that the novel strain could be readily distinguished from its closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, strain M1T represents a novel species of the genus Brachybacterium, for which the name Brachybacterium huguangmaarense sp. nov. is proposed. The type strain is M1T (=CCTCC AB 2012866T=DSM 26370T).
authors.affiliation: College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences/Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Haikou 571101, PR China.
authors.forename: Qing-Yi
authors.initials: QY
authors.lastname: Xie
authors.affiliation: Guangdong Huankai Microbial Sci. & Tech. Co., Ltd, Guangzhou 510663, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071 PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, PR China.
authors.forename: Jun-Ying
authors.initials: JY
authors.lastname: An
authors.affiliation: College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, PR China.
authors.forename: Ya-Mei
authors.initials: YM
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071 PR China.
authors.forename: Kui
authors.initials: K
authors.lastname: Hong
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D006017:Glycolipids / Q000737:chemistry; D060106:Lakes / Q000382:microbiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C062629:menaquinone 7