title: Effect of vitamin E and C supplementation on oxidative damage and total antioxidant capacity in lead-exposed workers.
abstract: The molecular response of the antioxidant system and the effects of antioxidant supplementation against oxidative insult in lead-exposed workers has not been sufficiently studied. In this work, antioxidants (vitamin E 400 IU+vitamin C 1g/daily) were supplemented for one year to 15 workers exposed to lead (73 μg of lead/dl of blood) and the results were compared with those on 19 non-lead exposed workers (6.7 μg of lead/dl). Lead intoxication was accompanied by a high oxidative damage and an increment in the erythrocyte antioxidant response due to increased activity of catalase and superoxide dismutase. Antioxidant supplementations decreased significantly the oxidative damage as well as the total antioxidant capacity induced by lead intoxication with reduction of the antioxidant enzyme activities. We conclude that antioxidant supplementation is effective in reducing oxidative damage and induces modifications in the physiopathological status of the antioxidant response in lead-exposed workers.
authors.affiliation: Biochemistry Department, Centro de Investigación y Estudios Avanzados IPN, México, DF, Mexico.
authors.forename: Adela-Leonor
authors.initials: AL
authors.lastname: Rendón-Ramírez
authors.affiliation: CIATEC, León, GTO, Mexico.
authors.forename: María
authors.initials: M
authors.lastname: Maldonado-Vega
authors.affiliation: Faculty of Medicine, UJED, Durango, DGO, Mexico.
authors.forename: Martha-Angelica
authors.initials: MA
authors.lastname: Quintanar-Escorza
authors.affiliation: Section of Methodology of Science, Centro de Investigación y Estudios Avanzados IPN, México, DF, Mexico.
authors.forename: Gerardo
authors.initials: G
authors.lastname: Hernández
authors.affiliation: HRAEB, León, GTO, Mexico.
authors.forename: Bertha-Isabel
authors.initials: BI
authors.lastname: Arévalo-Rivas
authors.affiliation: Cellular Physiology Institute, UNAM, Mexico.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Zentella-Dehesa
authors.affiliation: Biochemistry Department, Centro de Investigación y Estudios Avanzados IPN, México, DF, Mexico. Electronic address: jcalder@cinvestav.mx.
authors.forename: José-Víctor
authors.initials: JV
authors.lastname: Calderón-Salinas
mesh_terms: D000328:Adult; D000395:Air Pollutants, Occupational / Q000097:blood / Q000633:toxicity*; D000975:Antioxidants / Q000493:pharmacokinetics / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000097:blood / Q000493:pharmacokinetics / Q000494:pharmacology*; D002374:Catalase / Q000097:blood; D019587:Dietary Supplements; D005979:Glutathione Peroxidase / Q000097:blood; D005980:Glutathione Reductase / Q000097:blood; D006801:Humans; D007854:Lead / Q000097:blood / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D016273:Occupational Exposure / Q000009:adverse effects / Q000032:analysis; D018384:Oxidative Stress / Q000187:drug effects; D000623:Porphobilinogen Synthase / Q000097:blood; D013482:Superoxide Dismutase / Q000097:blood; D014810:Vitamin E / Q000097:blood / Q000493:pharmacokinetics / Q000494:pharmacology*
chemical_list: D000395:Air Pollutants, Occupational; D000975:Antioxidants; D014810:Vitamin E; D007854:Lead; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D000623:Porphobilinogen Synthase; D001205:Ascorbic Acid
keywords: Antioxidants; Blood; Enzymes; Human; Lead toxicity; Oxidation