title: Molecular cloning and expression of the gene for G protein alpha subunit induced by bisphenol A in marine polychaete Perinereis aibuhitensis.
abstract: A G protein alpha subunit gene named Pa Gα was isolated from the marine polychaete Perinereis aibuhitensis. The full-length cDNA of Pa Gα was 1832 bp and contained a 205 bp 5' untranslated region (5'UTR), a 565 bp 3' UTR and a 1062 bp open reading frame encoding 353 amino acid residues. The deduced protein sequence of Pa Gα showed 73% homology with the Gα protein of Dipolydora quadrilobata. Tissue-specific expression induced by exposure to bisphenol A (BPA) in P. aibuhitensis was detected by real-time PCR, which showed BPA induced expression of the Pa Gα gene, and the level of transcription was related positively to the concentration of BPA and the length of exposure time. With increasing concentration of BPA and length of exposure time, the level of mRNA transcription was raised gradually, but the level of increasing expression of Pa Gα mRNA induced by exposure to BPA varied significantly among different tissues.
authors.affiliation: Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning province, Dalian Ocean University, Dalian, 116023, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning province, Dalian Ocean University, Dalian, 116023, China. Electronic address: ybzhou2011@hotmail.com.
authors.forename: Yibing
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning province, Dalian Ocean University, Dalian, 116023, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning province, Dalian Ocean University, Dalian, 116023, China.
authors.forename: Shaojuan
authors.initials: S
authors.lastname: Li
authors.affiliation: Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning province, Dalian Ocean University, Dalian, 116023, China.
authors.forename: Dazuo
authors.initials: D
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001559:Benzhydryl Compounds / Q000633:toxicity*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D044385:GTP-Binding Protein alpha Subunits / Q000235:genetics*; D015870:Gene Expression; D008969:Molecular Sequence Data; D010636:Phenols / Q000633:toxicity*; D011077:Polychaeta / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D018076:DNA, Complementary; D044385:GTP-Binding Protein alpha Subunits; D010636:Phenols; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; G protein α subunit; Gene expression; Perinereis aibuhitensis