title: A nano-graphite-DNA hybrid sensor for magnified fluorescent detection of mercury(II) ions in aqueous solution.
abstract: In this communication, we present a nano-graphite-DNA hybrid sensor for fluorescent detection of mercury(II) ions in aqueous solution for the first time. Furthermore, an amplification strategy based on nano-graphite for Hg(2+) detection by using DNase I was demonstrated. The proposed amplified assay was simple and cost-effective with a limit of detection (LOD) for Hg(2+) of 0.5 nM, which was about 20-fold lower than that of traditional unamplified homogeneous assays. We further demonstrated its practical application to detect Hg(2+) in a real sample.
authors.affiliation: Research Center of Analytical Instrumentation, Analytical Testing Center, College of Chemistry, Sichuan University, Chengdu, China. yduan@scu.edu.cn.
authors.forename: Yin
authors.initials: Y
authors.lastname: Wei
authors.forename: Bianmiao
authors.initials: B
authors.lastname: Li
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.forename: Yixiang
authors.initials: Y
authors.lastname: Duan
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002413:Cations, Divalent; D004277:DNA, Single-Stranded / Q000737:chemistry*; D003850:Deoxyribonuclease I / Q000737:chemistry; D060766:Drinking Water / Q000032:analysis; D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D008628:Mercury / Q000032:analysis*; D053758:Nanoparticles / Q000737:chemistry*; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000032:analysis
chemical_list: D002413:Cations, Divalent; D004277:DNA, Single-Stranded; D060766:Drinking Water; D014874:Water Pollutants, Chemical; D006108:Graphite; D003850:Deoxyribonuclease I; D008628:Mercury