title: Clinical significance of serum ADP-ribosylation and NAD glycohydrolase activity in patients with colorectal cancer.
abstract: The objective of this study was to evaluate the clinical significance of serum ADP-ribosylation and NAD glycohydrolase activity in patients with colorectal cancer (CRC). A total of 108 patients with CRC who underwent curative surgery and 20 healthy volunteers were enrolled in this study. ADP-ribosylation and NAD glycohydrolase activity levels were determined. The association of ADP-ribosylation and NAD glycohydrolase with clinical and laboratory factors and their impact on overall survival (OS) and disease free survival (DFS) were shown. The preoperative ADP-ribosylation and NAD glycohydrolase activity levels were significantly higher in patients with CRC than in the control group (p<0.001). ADP-ribosylation and NAD glycohydrolase activity levels were correlated with tumor stage (p=0.05, p=0.001), stage of disease (p<0.001, p<0.001), serum CEA level (p<0.001, p<0.001), and site of lesion (p<0.001, p<0.001), respectively. Patients with high ADP-ribosylation had significantly unfavorable OS and DFS compared with those with lower levels (p<0.001, p<0.001), respectively. Moreover, the patients with high NAD glycohydrolase activity showed significantly worse OS and DFS rates, similar to ADP-ribosylation. Serum levels of ADP-ribosylation and NAD glycohydrolase activity correlate well with tumor stage, stage of disease, serum CEA level, and site of lesion. In conclusion, elevated levels of preoperative ADP-ribosylation and NAD glycohydrolase levels in serum are associated with poor prognosis in patients with CRC.
authors.affiliation: İstanbul Faculty of Medicine, Department of Biophysics, İstanbul University, Çapa-İstanbul, Turkey, basakgn@yahoo.com.
authors.forename: Başak
authors.initials: B
authors.lastname: Varol
authors.forename: Özlem
authors.initials: Ö
authors.lastname: Coşkun
authors.forename: Senem
authors.initials: S
authors.lastname: Karabulut
authors.forename: Kürşat Rahmi
authors.initials: KR
authors.lastname: Serin
authors.forename: Oktar
authors.initials: O
authors.lastname: Asoğlu
authors.forename: Işıl
authors.initials: I
authors.lastname: Albeniz
authors.forename: Faruk
authors.initials: F
authors.lastname: Taş
authors.forename: Rüstem
authors.initials: R
authors.lastname: Nurten
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002272:Carcinoembryonic Antigen / Q000097:blood; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000401:mortality* / Q000473:pathology; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009244:NAD+ Nucleosidase / Q000097:blood*; D009367:Neoplasm Staging
chemical_list: D002272:Carcinoembryonic Antigen; D000244:Adenosine Diphosphate; D009244:NAD+ Nucleosidase