title: Protective effects of resveratrol against di-n buthyl phthalate induced toxicity in ductus epididymis and ductus deferens in rats.
abstract: OBJECTIVE
  This study aimed to observe the possible protective effects of resveratrol (RSV) against damage induced by di-n-butylphthalate (DBP), on the ductus epididymis and deferens in rats.
  
  
  METHODS
  Six groups of rats were used in the experiment: Group 1: Control group; Group 2: Solvent (carboxymethylcellulose (CMC), 10 ml/kg); Group 3: 500 mg/kg/day DBP; Group 4: 500 mg/kg/day DBP+20 mg/kg/day RSV; Group 5: 1000 mg/kg/day DBP; Group 6: 1000 mg/kg/day DBP + 20 mg/kg/day RSV. Groups were treated by gavage for 30 days. Immunohistochemical, electronmicroscopic and histomorphometric examinations were carried out in the epididymis and deferens.
  
  
  RESULTS
  In the ductus epididymis and deferens mitochondrial crystolysis, exfoliation of the stereocilia and openings in lateral surface increased with DBP dosage, but these structures were recovered with RSV. DBP reduced the epithelial height of epididymis and vas deferens. Lumen dilatation was observed in both tissues. These disorders may lead to dysfunction of epithelial absorption. In the TUNEL examinations in both tissues, there were no apoptotic cells or apoptotic bodies.
  
  
  CONCLUSIONS
  In conclusion, DBP administration caused structural degeneration in the epididymis and deferens, parallel to dose evaluation and RSV can reverse these changes with its protective effects.
authors.affiliation: Histology and Embryology Department, Eskisehir Osmangazi Universty, Medicine School, Turkey.
authors.forename: Erhan
authors.initials: E
authors.lastname: Sahin
authors.affiliation: Histology and Embryology Department, Gazi Universty Medicine School, Turkey.
authors.forename: Celal
authors.initials: C
authors.lastname: Ilgaz
authors.affiliation: Histology and Embryology Department, Gazi Universty Medicine School, Turkey.
authors.forename: Deniz
authors.initials: D
authors.lastname: Erdoğan
authors.affiliation: Histology and Embryology Department, Gazi Universty Medicine School, Turkey.
authors.forename: Gülnur
authors.initials: G
authors.lastname: Take
authors.affiliation: Histology and Embryology Department, Gazi Universty Medicine School, Turkey.
authors.forename: Güleser
authors.initials: G
authors.lastname: Göktas
mesh_terms: D000818:Animals; D003993:Dibutyl Phthalate / Q000633:toxicity*; D004822:Epididymis / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D020287:In Situ Nick-End Labeling; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D014649:Vas Deferens / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure
chemical_list: D013267:Stilbenes; D003993:Dibutyl Phthalate; D000077185:Resveratrol
keywords: Di n buthyl phthalate; Ductus deferens; Ductus epididymis; Resveratrol