title: Toxoplasma gondii genotypes isolated from pregnant women with follow-up of infected children in southern Brazil.
abstract: BACKGROUND
  Toxoplasma gondii populations that perpetuate in South America's natural ecosystems display broad genetic diversity, but the impact of this diversity on humans is generally unknown. In this short communication, we depict the genotypic traits of four isolates related to congenital parasitism as it emerges in Southern Brazil.
  
  
  METHODS
  Using the PCR-restriction fragment length polymorphism markers SAG1, 5'3'SAG2, alt. SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico, and CS3. Three of the four strains (TgCTBral, TgCTBrv, and TgCTBrac) were identified as ToxoDB genotype #166.
  
  
  RESULTS
  Three children displayed normal cognitive/psychomotor development, and one child displayed Sabin's tetrad (TgCTBral).
  
  
  CONCLUSIONS
  Vertical transmission of the two genotypes was observed and contributes to knowledge of T. gondii strains isolated from humans in Brazil.
authors.affiliation: Department of Medicine, Teaching Hospital of State University of Maringá, 1590 Mandacaru Avenue, Maringá, Paraná, CEP 87083-240, Brazil.
authors.forename: Lourenço T
authors.initials: LT
authors.lastname: Higa
authors.forename: João L
authors.initials: JL
authors.lastname: Garcia
authors.forename: Chunlei
authors.initials: C
authors.lastname: Su
authors.forename: Rita C
authors.initials: RC
authors.lastname: Rossini
authors.forename: Ana L
authors.initials: AL
authors.lastname: Falavigna-Guilherme
mesh_terms: D000328:Adult; D001938:Brazil; D002648:Child; D003072:Cognition Disorders / Q000209:etiology; D016054:DNA, Protozoan / Q000235:genetics; D002658:Developmental Disabilities / Q000209:etiology; D005260:Female; D005500:Follow-Up Studies; D005819:Genetic Markers; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D018445:Infectious Disease Transmission, Vertical; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000469:parasitology; D014122:Toxoplasma / Q000235:genetics* / Q000302:isolation & purification; D014123:Toxoplasmosis / Q000150:complications / Q000469:parasitology* / Q000635:transmission
chemical_list: D016054:DNA, Protozoan; D005819:Genetic Markers
keywords: Brazil; Children; Genotyping; Pregnant women; Toxoplasmosis