title: A miR169 isoform regulates specific NF-YA targets and root architecture in Arabidopsis.
abstract: In plants, roots are essential for water and nutrient acquisition. MicroRNAs (miRNAs) regulate their target mRNAs by transcript cleavage and/or inhibition of protein translation and are known as major post-transcriptional regulators of various developmental pathways and stress responses. In Arabidopsis thaliana, four isoforms of miR169 are encoded by 14 different genes and target diverse mRNAs, encoding subunits A of the NF-Y transcription factor complex. These miRNA isoforms and their targets have previously been linked to nutrient signalling in plants. By using mimicry constructs against different isoforms of miR169 and miR-resistant versions of NF-YA genes we analysed the role of specific miR169 isoforms in root growth and branching. We identified a regulatory node involving the particular miR169defg isoform and NF-YA2 and NF-YA10 genes that acts in the control of primary root growth. The specific expression of MIM169defg constructs altered specific cell type numbers and dimensions in the root meristem. Preventing miR169defg-regulation of NF-YA2 indirectly affected laterial root initiation. We also showed that the miR169defg isoform affects NF-YA2 transcripts both at mRNA stability and translation levels. We propose that a specific miR169 isoform and the NF-YA2 target control root architecture in Arabidopsis.
authors.affiliation: Institut des Sciences du Végétal (ISV), CNRS, UPR2355, Saclay Plant Sciences, F-91198, Gif-sur-Yvette Cedex, France.|Université Paris Diderot, Sorbonne Paris Cité, F-75205, Paris Cedex 13, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Sorin
authors.affiliation: Institut des Sciences du Végétal (ISV), CNRS, UPR2355, Saclay Plant Sciences, F-91198, Gif-sur-Yvette Cedex, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Declerck
authors.affiliation: Institut des Sciences du Végétal (ISV), CNRS, UPR2355, Saclay Plant Sciences, F-91198, Gif-sur-Yvette Cedex, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Christ
authors.affiliation: Institut des Sciences du Végétal (ISV), CNRS, UPR2355, Saclay Plant Sciences, F-91198, Gif-sur-Yvette Cedex, France.|INRA, Institut JP Bourgin, Route de Saint-Cyr, 78026, Versailles Cedex, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Blein
authors.affiliation: Institut des Sciences du Végétal (ISV), CNRS, UPR2355, Saclay Plant Sciences, F-91198, Gif-sur-Yvette Cedex, France.
authors.forename: Linnan
authors.initials: L
authors.lastname: Ma
authors.affiliation: Institut des Sciences du Végétal (ISV), CNRS, UPR2355, Saclay Plant Sciences, F-91198, Gif-sur-Yvette Cedex, France.|Université Paris Diderot, Sorbonne Paris Cité, F-75205, Paris Cedex 13, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Lelandais-Brière
authors.affiliation: Department of Plant Systems Biology, Flanders Institute for Biotechnology, Technologiepark 927, 9052, Ghent, Belgium.|Department Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, 9052, Ghent, Belgium.
authors.forename: Maria Fransiska
authors.initials: MF
authors.lastname: Njo
authors.affiliation: Department of Plant Systems Biology, Flanders Institute for Biotechnology, Technologiepark 927, 9052, Ghent, Belgium.|Department Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, 9052, Ghent, Belgium.
authors.forename: Tom
authors.initials: T
authors.lastname: Beeckman
authors.affiliation: Institut des Sciences du Végétal (ISV), CNRS, UPR2355, Saclay Plant Sciences, F-91198, Gif-sur-Yvette Cedex, France.
authors.forename: Martin
authors.initials: M
authors.lastname: Crespi
authors.affiliation: Institut des Sciences du Végétal (ISV), CNRS, UPR2355, Saclay Plant Sciences, F-91198, Gif-sur-Yvette Cedex, France.|Université Paris Diderot, Sorbonne Paris Cité, F-75205, Paris Cedex 13, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Hartmann
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D023081:CCAAT-Binding Factor / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D018519:Meristem / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D010641:Phenotype; D018517:Plant Roots / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified; D059368:RNA Isoforms; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D023081:CCAAT-Binding Factor; C000591832:MIRN169 microRNA, Arabidopsis; D035683:MicroRNAs; C000591833:NF-YA2 protein, Arabidopsis; D059368:RNA Isoforms; D018749:RNA, Plant
keywords: Arabidopsis; Nuclear Factor Y subunit A (NF-YA); miR169 isoforms; mimicry; root branching