title: Analysis of high pIα-Amy-1gene family members expressed in late maturity α-amylase in wheat (Triticum aestivumL.).
abstract: Late maturity α-amylase (LMA) is a genetic defect involving the synthesis of high pI isozymes of α-amylase encoded byα-Amy-1genes during the later stages of grain development. The aims of this investigation were to determine both the number of expressedα-Amy-1genes and their relative transcript abundance. Sub-cloning and sequencing of expressed high pI α-amylase genes in developing wheat seeds revealed three insertion/deletion patterns in the 3' untranslated region and numerous single nucleotide polymorphisms at the 3' end ofα-Amy-1.The genetic variations defined 36α-Amy-1gene sequences that were expressed on the onset of LMA in doubled haploid progenies (SpM25, SpM52 and SpM127) derived from the cross Spica (LMA)/Maringa (non-LMA). Five isoelectric point groups were predicted based on the translated partial coding sequences. The potential application of quantitative real-time RT-PCR in screening wheat genotypes for LMA is discussed.
authors.affiliation: School of Agriculture Food and Wine, University of Adelaide, Waite Campus, Glen Osmond, SA 5064 Australia.
authors.forename: Cong-Rong
authors.initials: CR
authors.lastname: Cheng
authors.affiliation: School of Agriculture Food and Wine, University of Adelaide, Waite Campus, Glen Osmond, SA 5064 Australia ; Crop Improvement, Plant Genomics Centre, South Australian Research and Development Institute, Waite Campus, Urrbrae, SA 6064 Australia.
authors.forename: Klaus
authors.initials: K
authors.lastname: Oldach
authors.affiliation: School of Agriculture Food and Wine, University of Adelaide, Waite Campus, Glen Osmond, SA 5064 Australia.
authors.forename: Kolumbina
authors.initials: K
authors.lastname: Mrva
authors.affiliation: School of Agriculture Food and Wine, University of Adelaide, Waite Campus, Glen Osmond, SA 5064 Australia.
authors.forename: Daryl
authors.initials: D
authors.lastname: Mares
keywords: High pI α-amylase; Late maturity α-amylase (LMA); Quantitative real-time RT-PCR; Triticum aestivum; Wheat; α-Amy-1 genes