title: Modulation of immune function by milk fat globule membrane isolates.
abstract: The nutritional value and characterization of minor milk components on mammalian immune function are not fully understood. The aim of this research was to test the ability of a milk fat globule membrane (MFGM) isolate to modulate murine immune function in vitro, by studying its effects on splenocyte proliferation, apoptosis, and cytokine production. Proliferation of spleen cells was not affected by the MFGM isolate; however, in the presence of polyclonal activators, the MFGM isolate suppressed cell proliferation. Results obtained by flow cytometry did not support programmed cell death as the cause of the MFGM immune-modulating capacity. A mode of suppression on the splenocyte activation process was suggested from a marked decrease in the production of IFN-γ and tumor necrosis factor-α cytokines, typical indicators of immune cell activation. The effect of MFGM on IL-4 secretion was significantly less than that for the other 2 cytokines. The activity exerted by the MFGM over concanavalin A-stimulated cells differed from that observed in cells treated with lipopolysaccharide, suggesting a different mode of action depending on the activator used. These results indicate the potential of MFGM extracts as functional ingredients with bioactive modulating capacity.
authors.affiliation: Department of Food Science, University of Guelph, Guelph, ON, N1G 2W5 Canada.
authors.forename: R
authors.initials: R
authors.lastname: Zanabria
authors.affiliation: Department of Food Science, University of Guelph, Guelph, ON, N1G 2W5 Canada.
authors.forename: A M
authors.initials: AM
authors.lastname: Tellez
authors.affiliation: Department of Food Science, University of Guelph, Guelph, ON, N1G 2W5 Canada.
authors.forename: M
authors.initials: M
authors.lastname: Griffiths
authors.affiliation: Department of Pathobiology, University of Guelph, Guelph, ON, N1G 2W5 Canada.
authors.forename: S
authors.initials: S
authors.lastname: Sharif
authors.affiliation: Department of Food Science, University of Guelph, Guelph, ON, N1G 2W5 Canada. Electronic address: milena.corredig@uoguelph.ca.
authors.forename: M
authors.initials: M
authors.lastname: Corredig
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003208:Concanavalin A / Q000378:metabolism; D005260:Female; D006017:Glycolipids / Q000737:chemistry* / Q000276:immunology*; D006023:Glycoproteins / Q000737:chemistry* / Q000276:immunology*; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D066292:Lipid Droplets; D008070:Lipopolysaccharides / Q000009:adverse effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008892:Milk / Q000737:chemistry*; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D006017:Glycolipids; D006023:Glycoproteins; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; C110367:milk fat globule; D003208:Concanavalin A; D015847:Interleukin-4; D007371:Interferon-gamma
keywords: antiinflammatory; bioactivity; immunomodulation; milk fat globule membrane