title: Transgenic rice expressing rice stripe virus NS3 protein, a suppressor of RNA silencing, shows resistance to rice blast disease.
abstract: The NS3 protein of rice stripe virus (RSV), encoded by the virion strand of RNA3, is a viral suppressor of RNA silencing (VSR). Rice expressing NS3 had a normal phenotype, was initially sensitive to RSV but recovered at the later stages of infection. RSV accumulated slightly more in transgenic than in wild-type plants at the early stage of infection, but accumulation was similar later. Transgenic rice expressing NS3 also showed enhanced resistance to the fungus Magnaporthe oryzae. Meanwhile, expressional levels of genes related to the salicylic acid (SA) and jasmonic acid (JA) pathways were not significantly altered, indicating that the defense to M. oryzae was independent of the SA and JA pathways. We propose that NS3 may have dual functions, facilitating viral infection as a VSR and inhibiting pathogenic development as an inducer of host defense.
authors.affiliation: College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Gentu
authors.initials: G
authors.lastname: Wu
authors.forename: Jiaoyu
authors.initials: J
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.forename: Bo
authors.initials: B
authors.lastname: Dong
authors.forename: Yanli
authors.initials: Y
authors.lastname: Wang
authors.forename: Guochang
authors.initials: G
authors.lastname: Sun
authors.forename: Chengqi
authors.initials: C
authors.lastname: Yan
authors.forename: Fei
authors.initials: F
authors.lastname: Yan
authors.forename: Jianping
authors.initials: J
authors.lastname: Chen
mesh_terms: D060467:Disease Resistance; D015870:Gene Expression; D020082:Magnaporthe / Q000502:physiology*; D012275:Oryza / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D034622:RNA Interference; D029364:Tenuivirus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology
chemical_list: D014764:Viral Proteins