title: Heterogeneity of CD4+ and CD8+ T-cell responses to cytomegalovirus in HIV-infected and HIV-uninfected men who have sex with men.
abstract: Studies of T-cell immunity to human cytomegalovirus (CMV) primarily reflect anti-CMV pp65 or immediate early antigen 1 (IE-1) activity. We assessed responses of T cells from human immunodeficiency virus (HIV)-negative and HIV-infected men to peptide pools spanning 19 CMV open reading frames selected because they previously correlated with total CMV-specific T-cell responses in healthy donors. Cells producing cytokines in response to pp65 or IE-1 together composed <12% and <40% of the total CD4(+) and CD8(+) T-cell responses to CMV, respectively. These proportions were generally similar regardless of HIV serostatus. Thus, analyses of total CMV-specific T-cell responses should extend beyond pp65 and IE-1 regardless of HIV serostatus.
authors.affiliation: Division of Geriatrics Medicine and Gerontology, Johns Hopkins University School of Medicine.
authors.forename: Huifen
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.
authors.forename: Joseph B
authors.initials: JB
authors.lastname: Margolick
authors.affiliation: Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.
authors.forename: Jay H
authors.initials: JH
authors.lastname: Bream
authors.affiliation: Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.
authors.forename: Tricia L
authors.initials: TL
authors.lastname: Nilles
authors.affiliation: Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.
authors.forename: Susan
authors.initials: S
authors.lastname: Langan
authors.affiliation: Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.
authors.forename: Hanhvy T
authors.initials: HT
authors.lastname: Bui
authors.affiliation: Vaccine and Gene Therapy Institute and Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon.
authors.forename: Andrew W
authors.initials: AW
authors.lastname: Sylwester
authors.affiliation: Vaccine and Gene Therapy Institute and Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon.
authors.forename: Louis J
authors.initials: LJ
authors.lastname: Picker
authors.affiliation: Division of Geriatrics Medicine and Gerontology, Johns Hopkins University School of Medicine.
authors.forename: Sean X
authors.initials: SX
authors.lastname: Leng
mesh_terms: D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology*; D018414:CD8-Positive T-Lymphocytes / Q000502:physiology*; D003586:Cytomegalovirus Infections / Q000276:immunology*; D015658:HIV Infections / Q000150:complications* / Q000276:immunology*; D018451:Homosexuality, Male; D006801:Humans; D008297:Male
keywords: CD4+ and CD8+ T-cell responses; CMV; HIV infection; IE-1; IFN-γ; IL-2; MACS; TNF-α; intracellular cytokine staining; pp65