title: Polymerase chain reaction of enterococcus faecalis and candida albicans in apical periodontitis from Turkish patients.
abstract: OBJECTIVE
  The aim of this study was to determine the frequency of two important pathogenic microorganisms associated with endodontic infections, Enterococcus faecalis and Candida albicans, in root canal samples from patients with necrotic pulps or failed canal therapy by polymerase chain reaction method.
  
  
  METHODS
  Microbial samples were obtained from 117 teeth with necrotic pulp tissues and 114 teeth with failed endodontic treatment.
  
  
  RESULTS
  E.faecalis were identified in 16% of the necrotic and 10% of the retreated root canal infections by PCR. C.albicans genome were identified in 20% and 11% of the necrotic and retreated root canal infections, respectively, by PCR. The frequencies of microbiota were not statistically different between necrotic and retreatment groups (p > 0.05, chi squared test).
  
  
  CONCLUSIONS
  PCR analysis of teeth with periapical lesions revealed that E.faecalis was found in fewer patients than in previous studies. The C.albicans prevelance was consistent with previous reports. No statistical difference was found between primary and secondary root canal infections for C.albicans or E.faecalis. Key words:Primary root canal infection, secondary root canal infection, E.faecalis, C.albicans.
authors.affiliation: DDS, PhD. Assistant Professor, Cukurova University, Faculty of Dentistry, Department of Endodontics.
authors.forename: Aysin
authors.initials: A
authors.lastname: Dumani
authors.affiliation: DDS, PhD. Professor, Cukurova University, Faculty of Dentistry, Department of Endodontics.
authors.forename: Oguz
authors.initials: O
authors.lastname: Yoldas
authors.affiliation: DDS, PhD. Assistant Professor, Cukurova University, Faculty of Dentistry, Department of Endodontics.
authors.forename: Sehnaz
authors.initials: S
authors.lastname: Yilmaz
authors.affiliation: MD, PhD. Professor, Cukurova University, Department of Microbiology.
authors.forename: Fatih
authors.initials: F
authors.lastname: Koksal
authors.affiliation: DDS, PhD. Research Asistant, Cukurova University, Department of Microbiology.
authors.forename: Begum
authors.initials: B
authors.lastname: Kayar
authors.affiliation: DDS, PhD. Research Asistant, Cukurova University, Department of Microbiology.
authors.forename: Beril
authors.initials: B
authors.lastname: Akcimen
authors.affiliation: MD, PhD. Associated Professor, Cukurova University, Department of Biostatistic.
authors.forename: Gulsah
authors.initials: G
authors.lastname: Seydaoglu