title: Neural stem cells improve intracranial nanoparticle retention and tumor-selective distribution.
abstract: OBJECTIVE
  The purpose of this work is to determine if tumor-tropic neural stem cells (NSCs) can improve the tumor-selective distribution and retention of nanoparticles (NPs) within invasive brain tumors.
  
  
  METHODS
  Streptavidin-conjugated, polystyrene NPs are surface-coupled to biotinylated human NSCs. These NPs are large (798 nm), yet when conjugated to tropic cells, they are too large to passively diffuse through brain tissue or cross the blood-tumor barrier. NP distribution and retention was quantified 4 days after injections located either adjacent to an intracerebral glioma, in the contralateral hemisphere, or intravenously.
  
  
  CONCLUSIONS
  In all three in vivo injection paradigms, NSC-coupled NPs exhibited significantly improved tumor-selective distribution and retention over free-NP suspensions. These results provide proof-of-principle that NSCs can facilitate the tumor-selective distribution of NPs, a platform useful for improving intracranial drug delivery.
authors.affiliation: Department of Neurosciences, Beckman Research Institute at City of Hope, 1500 East Duarte Road, Duarte, CA, 91010, USA.
authors.forename: Rachael
authors.initials: R
authors.lastname: Mooney
authors.forename: Yiming
authors.initials: Y
authors.lastname: Weng
authors.forename: Revathiswari
authors.initials: R
authors.lastname: Tirughana-Sambandan
authors.forename: Valerie
authors.initials: V
authors.lastname: Valenzuela
authors.forename: Soraya
authors.initials: S
authors.lastname: Aramburo
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Garcia
authors.forename: Zhongqi
authors.initials: Z
authors.lastname: Li
authors.forename: Margarita
authors.initials: M
authors.lastname: Gutova
authors.forename: Alexander J
authors.initials: AJ
authors.lastname: Annala
authors.forename: Jacob M
authors.initials: JM
authors.lastname: Berlin
authors.forename: Karen S
authors.initials: KS
authors.lastname: Aboody
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D002470:Cell Survival; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism*; D005910:Glioma / Q000378:metabolism*; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism*; D009368:Neoplasm Transplantation; D058953:Neural Stem Cells / Q000502:physiology* / Q000637:transplantation; D010316:Particle Size; D014018:Tissue Distribution
chemical_list: D004337:Drug Carriers