title: Functional architecture of MFS D-glucose transporters.
abstract: The Major Facilitator Superfamily (MFS) is a diverse group of secondary transporters with over 10,000 members, found in all kingdoms of life, including Homo sapiens. One objective of determining crystallographic models of the bacterial representatives is identification and physical localization of residues important for catalysis in transporters with medical relevance. The recently solved crystallographic models of the D-xylose permease XylE from Escherichia coli and GlcP from Staphylococcus epidermidus, homologs of the human D-glucose transporters, the GLUTs (SLC2), provide information about the structure of these transporters. The goal of this work is to examine general concepts derived from the bacterial XylE, GlcP, and other MFS transporters for their relevance to the GLUTs by comparing conservation of functionally critical residues. An energy landscape for symport and uniport is presented. Furthermore, the substrate selectivity of XylE is compared with GLUT1 and GLUT5, as well as a XylE mutant that transports D-glucose.
authors.affiliation: Department of Physiology, Department of Microbiology, Immunology, and Molecular Genetics, and Molecular Biology Institute, University of California, Los Angeles, CA 90095-1662.
authors.forename: M Gregor
authors.initials: MG
authors.lastname: Madej
authors.forename: Linfeng
authors.initials: L
authors.lastname: Sun
authors.forename: Nieng
authors.initials: N
authors.lastname: Yan
authors.forename: H Ronald
authors.initials: HR
authors.lastname: Kaback
mesh_terms: D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D001692:Biological Transport / Q000502:physiology; D002151:Calorimetry; D018076:DNA, Complementary / Q000235:genetics; D019453:Escherichia coli O157 / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics; D051246:Glucose Transport Proteins, Facilitative / Q000737:chemistry*; D006801:Humans; D008081:Liposomes / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013379:Substrate Specificity; D027981:Symporters / Q000737:chemistry* / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D029968:Escherichia coli Proteins; D051246:Glucose Transport Proteins, Facilitative; D008081:Liposomes; D027981:Symporters; C033903:xylE protein, E coli
keywords: glucose transport; ligand docking; membrane proteins; sequence analysis; solute carrier transporter