title: Gram-scale purification of dehydroevodiamine from Evodia rutaecarpa fruits, and a procedure for selective removal of quaternary indoloquinazoline alkaloids from Evodia extracts.
abstract: Dehydroevodiamine (DHE) is a major bioactive constituent in the traditional Chinese herbal drug Evodiae fructus (Wu zhu yu). The compound has been shown to possess pronounced cardiovascular and neuropharmacological activities in vitro and in vivo. For quality control purposes and follow-up studies assessing potential safety risks of DHE, we developed a simple and efficient two-step protocol for gram-scale purification of DHE. An alkaloidal fraction was obtained by cation-exchange solid phase extraction, and DHE and the minor alkaloid hortiamine were purified by isocratic preparative RP-HPLC. The DHE content in different commercial batches of Evodiae fructus, and in a series of commercially available Evodia-containing TCM products was assessed. A daily intake of up to mg amounts of DHE was calculated from recommended doses of these products. A method for the selective removal of quaternary indoloquinazoline alkaloids from Evodia extracts was developed.
authors.affiliation: Division of Pharmaceutical Biology, University of Basel, Klingelbergstrasse 50, 4056 Basel, Switzerland.
authors.forename: Anja
authors.initials: A
authors.lastname: Schramm
authors.affiliation: Division of Pharmaceutical Biology, University of Basel, Klingelbergstrasse 50, 4056 Basel, Switzerland. Electronic address: matthias.hamburger@unibas.ch.
authors.forename: Matthias
authors.initials: M
authors.lastname: Hamburger
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D032086:Evodia / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D010946:Plants, Medicinal; D011799:Quinazolines / Q000302:isolation & purification
chemical_list: D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D011799:Quinazolines; C028633:dehydroevodiamine
keywords: Cation-exchange chromatography; Dehydroevodiamine; Evodia rutaecarpa; Gram-scale isolation; Preparative HPLC; Quantitative analysis