title: Synthesis, characterization, thermal and DNA-binding properties of new zinc complexes with 2-hydroxyphenones.
abstract: The neutral mononuclear zinc complexes with 2-hydroxyphenones (ketoH) having the formula [Zn(keto)2(H2O)2] and [Zn(keto)2(enR)], where enR stands for a N,N'-donor heterocyclic ligand such as 2,2'-bipyridine (bipy), 1,10-phenanthroline (phen) or 2,2'-dipyridylamine (dpamH), have been synthesized and characterized by IR, UV and (1)H NMR spectroscopies. The 2-hydroxyphenones are chelated to the metal ion through the phenolate and carbonyl oxygen atoms. The crystal structures of [bis(2-hydroxy-4-methoxy-benzophenone)(2,2'-bipyridine)zinc(II)] dimethanol solvate and [bis(2-hydroxy-benzophenone)(2,2'-bipyridine)zinc(II)] dimethanol solvate have been determined by X-ray crystallography. The thermal stability of the zinc complexes has been investigated by simultaneous TG/DTG-DTA technique. The ability of the complexes to bind to calf-thymus DNA (CT DNA) has been studied by UV-absorption and fluorescence emission spectroscopy as well as viscosity measurements. UV studies of the interaction of the complexes with DNA have shown that they can bind to CT DNA and the corresponding binding constants to DNA have been calculated and evaluated. The complexes most probably bind to CT DNA via intercalation as concluded by studying the viscosity of a DNA solution in the presence of the complexes. Competitive studies with ethidium bromide (EB) have shown that the reported complexes can displace the DNA-bound EB, suggesting strong competition with EB for the intercalation site.
authors.affiliation: Department of General and Inorganic Chemistry, Faculty of Chemistry, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece; Institute of Chemistry, Faculty of Sciences, University of Kragujevac, 34000 Kragujevac, Serbia.
authors.forename: Emina
authors.initials: E
authors.lastname: Mrkalić
authors.affiliation: Department of General and Inorganic Chemistry, Faculty of Chemistry, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece.
authors.forename: Ariadni
authors.initials: A
authors.lastname: Zianna
authors.affiliation: Department of General and Inorganic Chemistry, Faculty of Chemistry, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece.
authors.forename: George
authors.initials: G
authors.lastname: Psomas
authors.affiliation: Faculty of Chemistry, Adam Mickiewicz University, 61614 Poznan, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Gdaniec
authors.affiliation: Faculty of Chemistry, Adam Mickiewicz University, 61614 Poznan, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Czapik
authors.affiliation: Department of Organic Chemistry and Biochemistry, Faculty of Chemistry, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece.
authors.forename: Evdoxia
authors.initials: E
authors.lastname: Coutouli-Argyropoulou
authors.affiliation: Department of General and Inorganic Chemistry, Faculty of Chemistry, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece. Electronic address: lalia@chem.auth.gr.
authors.forename: Maria
authors.initials: M
authors.lastname: Lalia-Kantouri
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry*; D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002417:Cattle; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry; D004355:Drug Stability; D004996:Ethidium / Q000737:chemistry; D007364:Intercalating Agents / Q000138:chemical synthesis / Q000737:chemistry*; D010618:Phenanthrolines / Q000737:chemistry*; D013696:Temperature; D015032:Zinc / Q000737:chemistry*
chemical_list: D056831:Coordination Complexes; D007364:Intercalating Agents; D010618:Phenanthrolines; D015082:2,2'-Dipyridyl; D004247:DNA; C427203:calf thymus DNA; D004996:Ethidium; D015032:Zinc; C025205:1,10-phenanthroline; C048062:2,2'-dipyridylamine
keywords: 2-Hydroxyphenones; Competitive studies with ethidium bromide; Crystal structure; Interaction with calf-thymus DNA; Thermal behavior; Zinc complexes