title: [Study of the expression and function of Syk and VEGF-C in the development of non-small cell lung cancer].
abstract: OBJECTIVE
  To explore the role of Syk and VEGF-C in the development of NSCLC.
  
  
  METHODS
  Transfered these genes(eukaryotic expression vector pcDNA3.1-VEGF-C and pLNCX-syk)into A549 cells with the liposomes. Tested the expression of VEGF-C mRNA and Syk mRNA with RT-PCR.Investigated the cell invasion assay with transwell chamber in vitro. Analysis the expression of VEGF-C proteins in A549 cells and detect Syk and VEGF-C proteins of 81 NSCLC tissue samples with immunohistochemical staining.
  
  
  RESULTS
  Higher expression of VEGF-C was revealed in VEGF-C-construct-transfected A549 cell than that in controls through RT-PCR (P < 0.05) and immunohistochemistry(P < 0.01).RT-PCR also revealed that Syk expression was higher in Syk-construct-transfected cells than in controls (P < 0.05). The cell invading experiments showed that there was more invaded cells in both transfected terms than in controls (P < 0.05). The expression of Syk protein in NSCLC tissue were significantly lower than that in the normal lung tissue (P < 0.05), while the expression of VEGF-C protein in NSCLC tissue were significantly higher than that in the normal lung tissue(P < 0.05). The expression of Syk and VEGF-C has a negative correlationship (r = -1.000, P = 0.019).
  
  
  CONCLUSIONS
  The expression of Syk and VEGF-C has a negative correlationship in NSCLC tissue, VEGF-C-construct-transfected A549 cells are more aggressive than Syk-construct-transfected cells. And they may cooperated with each other in the development of NSCLC.
authors.affiliation: Department of Surgery, the Second Hospital of Shandong University,Jinan 250033, China.
authors.forename: Jia-zhong
authors.initials: JZ
authors.lastname: Guo
authors.affiliation: Department of Surgery, the Second Hospital of Shandong University,Jinan 250033, China.
authors.forename: Qi-feng
authors.initials: QF
authors.lastname: Sun
authors.affiliation: Department of Surgery, the Second Hospital of Shandong University,Jinan 250033, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Cong
authors.affiliation: Department of Surgery, the Second Hospital of Shandong University,Jinan 250033, China.
authors.forename: Yun-peng
authors.initials: YP
authors.lastname: Zhao
authors.affiliation: Department of Surgery, the Second Hospital of Shandong University,Jinan 250033, China.
authors.forename: Chuan-liang
authors.initials: CL
authors.lastname: Peng
authors.affiliation: Department of Surgery, the Second Hospital of Shandong University,Jinan 250033, China. Email:menglin2002@163.com.
authors.forename: Xiao-gang
authors.initials: XG
authors.lastname: Zhao
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D000072377:Syk Kinase; D014162:Transfection; D042582:Vascular Endothelial Growth Factor C / Q000235:genetics / Q000378:metabolism*
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C467486:VEGFC protein, human; D042582:Vascular Endothelial Growth Factor C; D011505:Protein-Tyrosine Kinases; C000606896:SYK protein, human; D000072377:Syk Kinase