title: An evaluation of the inflammatory response of lipopolysaccharide-treated primary dental pulp cells with regard to calcium silicate-based cements.
abstract: This study compared the biological changes of lipopolysaccharide (LPS)-treated dental pulp (DP) cells directly cultured on mineral trioxide aggregate (MTA) and calcium silicate (CS) cements. DP cells were treated with LPS for 24 h. Then, the LPS-treated DP cells were cultured on MTA or CS cements. Cell viability, cell death mechanism and interleukin (IL)-1β expressions were analysed. A one-way analysis of variance was used to evaluate the significance of the differences between the means. A significantly higher IL-1β expression (2.9-fold) was found for LPS-treated cells (P<0.05) compared with DP cells without LPS treatment at 24 h. Absorbance values of LPS-treated cells cultured on CS cement were higher than a tissue culture plate. A significant difference (P<0.05) in cell viability was observed between cells on CS and MTA cements 24 h after seeding. At 48 h, a high concentration of Si (5 mM) was released from MTA, which induced LPS-treated DP cell apoptosis. The present study demonstrates that CS cement is biocompatible with cultured LPS-treated DP cells. MTA stimulates inflammation in LPS-treated DP cells, which leads to greater IL-1β expression and apoptosis.
authors.affiliation: 1] School of Dentistry, Chung Shan Medical University, Taichung City, Taiwan [2] Department of Dentistry, Chung Shan Medical University Hospital, Taichung City, Taiwan.
authors.forename: Wei-Yun
authors.initials: WY
authors.lastname: Lai
authors.affiliation: 1] School of Dentistry, Chung Shan Medical University, Taichung City, Taiwan [2] Department of Dentistry, Chung Shan Medical University Hospital, Taichung City, Taiwan.
authors.forename: Chia-Tze
authors.initials: CT
authors.lastname: Kao
authors.affiliation: 1] School of Dentistry, Chung Shan Medical University, Taichung City, Taiwan [2] Department of Dentistry, Chung Shan Medical University Hospital, Taichung City, Taiwan.
authors.forename: Chi-Jr
authors.initials: CJ
authors.lastname: Hung
authors.affiliation: 1] School of Dentistry, Chung Shan Medical University, Taichung City, Taiwan [2] Department of Dentistry, Chung Shan Medical University Hospital, Taichung City, Taiwan.
authors.forename: Tsui-Hsien
authors.initials: TH
authors.lastname: Huang
authors.affiliation: Institute of Oral Science, Chung Shan Medical University, Taichung City, Taiwan.
authors.forename: Ming-You
authors.initials: MY
authors.lastname: Shie
mesh_terms: D017610:Calcium Compounds; D003738:Dental Cements; D003782:Dental Pulp / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000139:chemically induced* / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D017640:Silicates
chemical_list: D017610:Calcium Compounds; D003738:Dental Cements; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D017640:Silicates; C031293:calcium silicate