title: Heavy metals induce oxidative stress and genome-wide modulation in transcriptome of rice root.
abstract: Industrial growth, ecological disturbances and agricultural practices have contaminated the soil and water with many harmful compounds, including heavy metals. These heavy metals affect growth and development of plants as well as cause severe human health hazards through food chain contamination. In past, studies have been made to identify biochemical and molecular networks associated with heavy metal toxicity and uptake in plants. Studies suggested that most of the physiological and molecular processes affected by different heavy metals are similar to those affected by other abiotic stresses. To identify common and unique responses by different metals, we have studied biochemical and genome-wide modulation in transcriptome of rice (IR-64 cultivar) root after exposure to cadmium (Cd), arsenate [As(V)], lead (Pb) and chromium [Cr(VI)] in hydroponic condition. We observed that root tissue shows variable responses for antioxidant enzyme system for different heavy metals. Genome-wide expression analysis suggests variable number of genes differentially expressed in root in response to As(V), Cd, Pb and Cr(VI) stresses. In addition to unique genes, each heavy metal modulated expression of a large number of common genes. Study also identified cis-acting regions of the promoters which can be determinants for the modulated expression of the genes in response to different heavy metals. Our study advances understanding related to various processes and networks which might be responsible for heavy metal stresses, accumulation and detoxification.
authors.affiliation: Council of Scientific and Industrial Research, National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow, 226001, India.
authors.forename: Sonali
authors.initials: S
authors.lastname: Dubey
authors.forename: Manju
authors.initials: M
authors.lastname: Shri
authors.forename: Prashant
authors.initials: P
authors.lastname: Misra
authors.forename: Deepika
authors.initials: D
authors.lastname: Lakhwani
authors.forename: Sumit Kumar
authors.initials: SK
authors.lastname: Bag
authors.forename: Mehar H
authors.initials: MH
authors.lastname: Asif
authors.forename: Prabodh Kumar
authors.initials: PK
authors.lastname: Trivedi
authors.forename: Rudro Deo
authors.initials: RD
authors.lastname: Tripathi
authors.forename: Debasis
authors.initials: D
authors.lastname: Chakrabarty
mesh_terms: D001149:Arsenates / Q000633:toxicity; D002104:Cadmium / Q000633:toxicity; D002857:Chromium / Q000633:toxicity; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D018527:Hydroponics; D007854:Lead / Q000633:toxicity; D053858:Metabolic Networks and Pathways / Q000187:drug effects / Q000235:genetics; D012275:Oryza / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D018749:RNA, Plant / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D059467:Transcriptome; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D001149:Arsenates; D010940:Plant Proteins; D018749:RNA, Plant; D014874:Water Pollutants, Chemical; D002104:Cadmium; D002857:Chromium; D007854:Lead; C025657:arsenic acid