title: UVB irradiation does not directly induce detectable changes of DNA methylation in human keratinocytes.
abstract: Unprotected exposure to UVB radiation from the sun and the resulting DNA damage are thought to be responsible for physiological changes in the skin and for a variety of skin cancers, including basal cell and squamous cell carcinoma and malignant melanoma. Although the mutagenic effects of UVB have been well documented and studied mechanistically, there is only limited information as to whether UV light may also be responsible for inducing epigenetic changes in the genome of exposed cells. DNA methylation is a stable epigenetic modification involved in gene control. To study the effects of UVB radiation on DNA methylation, we repeatedly exposed normal human keratinocytes to a UVB light source. After a recovery period, we analyzed global DNA methylation patterns in the irradiated and control cells using the methylated-CpG island recovery assay (MIRA) method in combination with high-resolution microarrays. Bioinformatics analysis revealed only a limited number of possible differences between UVB-exposed and control cells. However, these minor apparent changes could not be independently confirmed by bisulfite sequencing-based approaches. This study reveals that UVB irradiation of keratinocytes has no recognizable global effect on DNA methylation patterns and suggests that changes in DNA methylation, as observed in skin cancers, are not immediate consequences of human exposure to solar UVB irradiation.
authors.affiliation: Department of Cancer Biology, Beckman Research Institute, City of Hope, Duarte, 91010, USA.
authors.forename: Christoph
authors.initials: C
authors.lastname: Lahtz
authors.affiliation: Department of Cancer Biology, Beckman Research Institute, City of Hope, Duarte, 91010, USA.
authors.forename: Sang-In
authors.initials: SI
authors.lastname: Kim
authors.affiliation: Department of Cancer Biology, Beckman Research Institute, City of Hope, Duarte, 91010, USA.
authors.forename: Steven E
authors.initials: SE
authors.lastname: Bates
authors.affiliation: Department of Information Sciences, Beckman Research Institute, City of Hope, Duarte, 91010, USA.
authors.forename: Arthur X
authors.initials: AX
authors.lastname: Li
authors.affiliation: Department of Molecular Medicine, Beckman Research Institute, City of Hope, Duarte, 91010, USA.
authors.forename: Xiwei
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Cancer Biology, Beckman Research Institute, City of Hope, Duarte, 91010, USA.
authors.forename: Gerd P
authors.initials: GP
authors.lastname: Pfeifer