title: A novel in vivo platform for studying alveolar bone regeneration in rat.
abstract: Alveolar bone regeneration is a significant challenge in dental implantation. Novel biomaterials and tissue-engineered constructs are under extensive development and awaiting in vivo animal tests to find clinical endpoint. Here, we establish a novel in vivo model, modifying gingivoperiosteoplasty in rat for the alveolar bone regeneration. Rat premaxillary bone defects were filled with silk scaffold or remained empty during the implantation period (up to 6 weeks), and harvested samples were analyzed by micro-computed tomography and histopathology. Empty defects showed increased but limited new bone formation with increasing implantation period. In defects implanted with silk sponge, the bone formation was significantly greater than that of empty defect, indicating an effective role of silk scaffold in the defect model. The modified premaxillary defect model in rat is simple to perform, while mimicking the clinical conditions, finding usefulness for the development of biomaterials and tissue-engineered constructs targeting alveolar bone regeneration in dental implantation.
authors.affiliation: Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, Republic of Korea ; Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea.
authors.forename: Joong-Hyun
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea ; Department of Conservative Dentistry, Dankook University Dental College, Cheonan, Republic of Korea.
authors.forename: Ho-Jin
authors.initials: HJ
authors.lastname: Moon
authors.affiliation: Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, Republic of Korea ; Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea.
authors.forename: Tae-Hyun
authors.initials: TH
authors.lastname: Kim
authors.affiliation: Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, Republic of Korea ; Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea.
authors.forename: Jong-Min
authors.initials: JM
authors.lastname: Jo
authors.affiliation: Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, Republic of Korea ; Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea.
authors.forename: Sung Hee
authors.initials: SH
authors.lastname: Yang
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, India.
authors.forename: Deboki
authors.initials: D
authors.lastname: Naskar
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, India.
authors.forename: Subhas C
authors.initials: SC
authors.lastname: Kundu
authors.affiliation: Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea ; Faculty of Pharmacy, The University of Sydney, Sydney, NSW, Australia.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Chrzanowski
authors.affiliation: Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, Republic of Korea ; Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea ; Department of Biomaterials Science, Dankook University Dental College, Cheonan, Republic of Korea.
authors.forename: Hae-Won
authors.initials: HW
authors.lastname: Kim
keywords: Animal model; bone regeneration; dental implantation; rat alveolar bone; silk