title: Alterations in the rat serum proteome induced by prepubertal exposure to bisphenol a and genistein.
abstract: Humans are exposed to an array of chemicals via the food, drink and air, including a significant number that can mimic endogenous hormones. One such chemical is Bisphenol A (BPA), a synthetic chemical that has been shown to cause developmental alterations and to predispose for mammary cancer in rodent models. In contrast, the phytochemical genistein has been reported to suppress chemically induced mammary cancer in rodents, and Asians ingesting a diet high in soy containing genistein have lower incidence of breast and prostate cancers. In this study, we sought to: (1) identify protein biomarkers of susceptibility from blood sera of rats exposed prepubertally to BPA or genistein using Isobaric Tandem Mass Tags quantitative mass spectrometry (TMT-MS) combined with MudPIT technology and, (2) explore the relevance of these proteins to carcinogenesis. Prepubertal exposures to BPA and genistein resulted in altered expression of 63 and 28 proteins in rat sera at postnatal day (PND) 21, and of 9 and 18 proteins in sera at PND35, respectively. This study demonstrates the value of using quantitative proteomic techniques to explore the effect of chemical exposure on the rat serum proteome and its potential for unraveling cellular targets altered by BPA and genistein involved in carcinogenesis.
authors.affiliation: Department of Pharmacology and Toxicology, University of Alabama at Birmingham (UAB) , 1670 University Boulevard, Volker Hall 241, Birmingham 35294, Alabama, United States.
authors.forename: Angela
authors.initials: A
authors.lastname: Betancourt
authors.forename: James A
authors.initials: JA
authors.lastname: Mobley
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Sarah
authors.initials: S
authors.lastname: Jenkins
authors.forename: Dongquan
authors.initials: D
authors.lastname: Chen
authors.forename: Kyoko
authors.initials: K
authors.lastname: Kojima
authors.forename: Jose
authors.initials: J
authors.lastname: Russo
authors.forename: Coral A
authors.initials: CA
authors.lastname: Lamartiniere
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000831:Animals, Newborn; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001559:Benzhydryl Compounds / Q000494:pharmacology*; D001798:Blood Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D063646:Carcinogenesis / Q000235:genetics; D002273:Carcinogens / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D019833:Genistein / Q000494:pharmacology; D006801:Humans; D007774:Lactation / Q000187:drug effects; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D015674:Mammary Neoplasms, Animal / Q000097:blood* / Q000139:chemically induced / Q000235:genetics / Q000473:pathology; D018811:Maternal Exposure; D058977:Molecular Sequence Annotation; D010636:Phenols / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000098826:Bisphenol A Compounds
chemical_list: D016588:Anticarcinogenic Agents; D001559:Benzhydryl Compounds; D001798:Blood Proteins; D002273:Carcinogens; D019833:Genistein; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds