title: Effect of miR-205 on 3T3-L1 preadipocyte differentiation through targeting to glycogen synthase kinase 3 beta.
abstract: MiR-205 plays an important role during adipogenesis by modulating the Wnt signaling pathway. Here, we report that miR-205 can regulate the differentiation of 3T3-L1 preadipocyte cells by targeting glycogen synthase kinase 3 beta (GSK-3β), which is a negative regulatory factor of Wnt signaling. When transiently overexpressed in 3T3-L1 cells, miR-205 suppressed the translation of GSK-3β, resulting in increased expression of β-catenin, which can promote cell proliferation by facilitating the transcription of the Wnt target genes cyclin D1 and c-Myc. However, stable overexpression of miR-205 in 3T3-L1 cells did not show any apparent inhibitory effect on adipogenic differentiation. While endogenous miR-205 was inhibited in 3T3-L1 cells, the adipogenesis marker gene, C/EBPα, was significantly activated and more lipid droplets appeared in differentiated adipocytes. However, systemic silencing of miR-205 in mice by using a locked-nucleic-acid-modified oligonucleotide (LNA-antimiR) did not lead to any observable increase in adipose tissue differentiation, implying that, as opposed to the findings from 3T3-L1 cells, miR-205 is dispensable for adipose tissue development in mice.
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510006, People's Republic of China.
authors.forename: Jingwei
authors.initials: J
authors.lastname: Yu
authors.forename: Yaosheng
authors.initials: Y
authors.lastname: Chen
authors.forename: Limei
authors.initials: L
authors.lastname: Qin
authors.forename: Luxi
authors.initials: L
authors.lastname: Cheng
authors.forename: Guangcai
authors.initials: G
authors.lastname: Ren
authors.forename: Peiqing
authors.initials: P
authors.lastname: Cong
authors.forename: Delin
authors.initials: D
authors.lastname: Mo
authors.forename: Zuyong
authors.initials: Z
authors.lastname: He
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000502:physiology*; D032761:Animal Experimentation; D000818:Animals; D002454:Cell Differentiation; D005786:Gene Expression Regulation; D038362:Glycogen Synthase Kinase 3 / Q000096:biosynthesis* / Q000235:genetics; D000071679:Glycogen Synthase Kinase 3 beta; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D013234:Stem Cells / Q000502:physiology*
chemical_list: C576666:MIRN205 microRNA, mouse; D035683:MicroRNAs; D000071679:Glycogen Synthase Kinase 3 beta; C000605507:Gsk3b protein, mouse; D038362:Glycogen Synthase Kinase 3