title: Making Drosophila lineage-restricted drivers via patterned recombination in neuroblasts.
abstract: The Drosophila cerebrum originates from about 100 neuroblasts per hemisphere, with each neuroblast producing a characteristic set of neurons. Neurons from a neuroblast are often so diverse that many neuron types remain unexplored. We developed new genetic tools that target neuroblasts and their diverse descendants, increasing our ability to study fly brain structure and development. Common enhancer-based drivers label neurons on the basis of terminal identities rather than origins, which provides limited labeling in the heterogeneous neuronal lineages. We successfully converted conventional drivers that are temporarily expressed in neuroblasts, into drivers expressed in all subsequent neuroblast progeny. One technique involves immortalizing GAL4 expression in neuroblasts and their descendants. Another depends on loss of the GAL4 repressor, GAL80, from neuroblasts during early neurogenesis. Furthermore, we expanded the diversity of MARCM-based reagents and established another site-specific mitotic recombination system. Our transgenic tools can be combined to map individual neurons in specific lineages of various genotypes.
authors.affiliation: 1] Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, USA. [2] Kyorin University School of Medicine, Mitaka, Tokyo, Japan. [3].
authors.forename: Takeshi
authors.initials: T
authors.lastname: Awasaki
authors.affiliation: 1] Department of Neurobiology, University of Massachusetts, Worcester, Massachusetts, USA. [2] [3].
authors.forename: Chih-Fei
authors.initials: CF
authors.lastname: Kao
authors.affiliation: 1] Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, USA. [2].
authors.forename: Ying-Jou
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: 1] Department of Neurobiology, University of Massachusetts, Worcester, Massachusetts, USA. [2].
authors.forename: Ching-Po
authors.initials: CP
authors.lastname: Yang
authors.affiliation: Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, USA.
authors.forename: Yaling
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, USA.
authors.forename: Barret D
authors.initials: BD
authors.lastname: Pfeiffer
authors.affiliation: Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, USA.
authors.forename: Haojiang
authors.initials: H
authors.lastname: Luan
authors.affiliation: Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, USA.
authors.forename: Xiaotang
authors.initials: X
authors.lastname: Jing
authors.affiliation: Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, USA.
authors.forename: Yu-Fen
authors.initials: YF
authors.lastname: Huang
authors.affiliation: Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, USA.
authors.forename: Yisheng
authors.initials: Y
authors.lastname: He
authors.affiliation: Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, USA.
authors.forename: Mark David
authors.initials: MD
authors.lastname: Schroeder
authors.affiliation: Neural Cell-Fate Determinants Section, National Institute of Neurological Disorders and Stroke, US National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Alexander
authors.initials: A
authors.lastname: Kuzin
authors.affiliation: Neural Cell-Fate Determinants Section, National Institute of Neurological Disorders and Stroke, US National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Thomas
authors.initials: T
authors.lastname: Brody
authors.affiliation: Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, USA.
authors.forename: Christopher T
authors.initials: CT
authors.lastname: Zugates
authors.affiliation: Neural Cell-Fate Determinants Section, National Institute of Neurological Disorders and Stroke, US National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Ward F
authors.initials: WF
authors.lastname: Odenwald
authors.affiliation: 1] Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, USA. [2] Department of Neurobiology, University of Massachusetts, Worcester, Massachusetts, USA.
authors.forename: Tzumin
authors.initials: T
authors.lastname: Lee
mesh_terms: D000818:Animals; D019070:Cell Lineage / Q000502:physiology; D054022:Cerebrum / Q000166:cytology* / Q000502:physiology; D004330:Drosophila / Q000166:cytology* / Q000502:physiology; D029721:Drosophila Proteins / Q000096:biosynthesis / Q000235:genetics; D005821:Genetic Techniques; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D051880:Receptors, Notch / Q000096:biosynthesis / Q000235:genetics; D011995:Recombination, Genetic; D019076:Transgenes
chemical_list: D029721:Drosophila Proteins; C497759:N protein, Drosophila; D051880:Receptors, Notch