title: Persistent detection of Plasmodium falciparum, P. malariae, P. ovale curtisi and P. ovale wallikeri after ACT treatment of asymptomatic Ghanaian school-children.
abstract: Two hundred and seventy four asymptomatic Ghanaian school-children aged 5 to 17 years were screened for malaria parasites by examination of blood films. One hundred and fifty five microscopically-positive individuals were treated with dihydroartemisinin-piperaquine and followed for 3 weeks. Retrospective species-specific PCR of all 274 screened samples identified an additional 60 children with sub-patent parasitaemia, and a substantial proportion of co-infections with Plasmodium malariae, Plasmodium ovale curtisi and Plasmodium ovale wallikeri. One hundred individuals harboured at least one non-falciparum parasite species. Using standard double-read microscopy, the 21-day efficacy of treatment against Plasmodium falciparum was 91.4% among the 117 children seen at all 5 visits. Using nested PCR to test 152 visit 5 blood samples, 22 were found to be parasite-positive. Twenty individuals harboured P. falciparum, four harboured P. ovale spp. and two P. malariae, with four of these 22 isolates being mixed species infections. The persistent detection of low density Plasmodium sp. infections following antimalarial treatment suggests these may be a hitherto unrecognised obstacle to malaria elimination.
authors.affiliation: Dept. of Immunology & Infection, London School of Hygiene & Tropical Medicine, London, UK.
authors.forename: Bismarck
authors.initials: B
authors.lastname: Dinko
authors.affiliation: Dept. of Immunology & Infection, London School of Hygiene & Tropical Medicine, London, UK.
authors.forename: Mary C
authors.initials: MC
authors.lastname: Oguike
authors.affiliation: Dept. of Biology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
authors.forename: John A
authors.initials: JA
authors.lastname: Larbi
authors.affiliation: Dept. of Immunology & Infection, London School of Hygiene & Tropical Medicine, London, UK ; Dept. of Medical Microbiology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.
authors.forename: Teun
authors.initials: T
authors.lastname: Bousema
authors.affiliation: Dept. of Immunology & Infection, London School of Hygiene & Tropical Medicine, London, UK ; Dept. of Clinical Parasitology, Hospital for Tropical Diseases, UCL Hospitals Trust, London, UK.
authors.forename: Colin J
authors.initials: CJ
authors.lastname: Sutherland
keywords: ACT; Asymptomatic malaria; Plasmodium malariae; Plasmodium ovale spp.