title: Obestatin changes proliferation, differentiation and apoptosis of porcine preadipocytes.
abstract: Obestatin, originally identified and purified from rat stomach extracts, was reported to bind to orphan G protein-coupled receptor, GPR39, and inhibit appetite and gastric motility. This study was conducted to investigate the effects of porcine obestatin on proliferation, differentiation and apoptosis of porcine preadipocytes isolated from subcutaneous fat of piglets. At indicated times of culture, morphology of preadipocytes and accumulated lipid droplets within the cells were identified by invert microscope. After treating with obestatin (0, 0.1, 1, 10 and 100nM), cell proliferation was measured by MTT method and protein expression of CCAAT/enhancer binding protein-α (C/EBPα), peroxisome proliferator-activated receptor-γ (PPARγ), Caspase-7 and Caspase-9 was determined by Western Blot, mRNA expression of GPR39 and Caspase-3 was analyzed by RT-PCR, and the activity of Caspase-3 was measured by spectrophotometric method. The results showed that obestatin had no effect on GPR39 expression, while promotes the optical density (OD) value of cells, enhanced protein expression of PPARγ and C/EBPa, decreased mRNA expression and activity of Caspase-3, and inhibited protein expression of Caspase-7 and Caspase-9 in a dose-dependent manner. These results suggested that obestatin enhances proliferation and differentiation of preadipocytes promoting PPARγ and C/EBPa expression, and inhibiting preadipocyte apoptosis by decreasing expression of Caspase-3, Caspase-7 and Caspase-9.
authors.affiliation: College of Yingdong agricultural science and engineering, Shaoguan university, Daxue road, Zhenjiang district, Shaoguan 512005, China.
authors.forename: Shengqiu
authors.initials: S
authors.lastname: Tang
authors.affiliation: College of Yingdong agricultural science and engineering, Shaoguan university, Daxue road, Zhenjiang district, Shaoguan 512005, China. Electronic address: dongcathy@163.com.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Dong
authors.affiliation: Hubei Key laboratory of animal embryo and molecular breeding, Hubei academy of agricultural science, Wuhan 430064, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000096:biosynthesis / Q000235:genetics; D053148:Caspase 3 / Q000096:biosynthesis / Q000235:genetics; D053179:Caspase 7 / Q000096:biosynthesis / Q000235:genetics; D053453:Caspase 9 / Q000096:biosynthesis / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D054439:Ghrelin / Q000494:pharmacology* / Q000502:physiology; D047495:PPAR gamma / Q000096:biosynthesis / Q000235:genetics; D061251:Primary Cell Culture; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis / Q000235:genetics; D050151:Subcutaneous Fat / Q000166:cytology; D034421:Sus scrofa
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; D054439:Ghrelin; D047495:PPAR gamma; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; D053148:Caspase 3; D053179:Caspase 7; D053453:Caspase 9