title: Identification of off-target cleavage sites of zinc finger nucleases and TAL effector nucleases using predictive models.
abstract: Using engineered nucleases, such as Zinc Finger Nucleases (ZFNs) or Transcription Activator-Like Effector Nucleases (TALENs), to make targeted genomic modifications has become a common technique to create new model organisms and custom cell lines, and has shown great promise for disease treatment. However, these nucleases have the potential for off-target cleavage that could confound interpretation of experimental results and be detrimental for therapeutic use. Here, we describe a method to test for nuclease cleavage at potential off-target sites predicted by bioinformatics models.
authors.affiliation: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
authors.forename: Eli J
authors.initials: EJ
authors.lastname: Fine
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Cradick
authors.forename: Gang
authors.initials: G
authors.lastname: Bao
mesh_terms: D000818:Animals; D019295:Computational Biology / Q000379:methods*; D053837:DNA Cleavage; D004720:Endonucleases / Q000378:metabolism*; D013379:Substrate Specificity; D016335:Zinc Fingers / Q000502:physiology*
chemical_list: D004720:Endonucleases