title: [Simultaneous determination of four common nonprotein nitrogen substances in urine by high performance liquid chromatography].
abstract: A high performance liquid chromatographic (HPLC) method was proposed to simultaneously determine four common nonprotein nitrogen substances, including creatine (Cr), creatinine (Cn), uric acid (Ua) and pseudouridine (Pu) in urine. After proteins being removed by acetone precipitation method, freeze drying and redissolving, the urine samples were analyzed by HPLC. Chromatographic separation was performed on a Waters RP18 Column (150 mm x 4.60 mm, 3.5 microm) in gradient elution mode using 10.0 mmol/L KH2PO4 solution (pH 4.78) and acetonitrile as mobile phases at a flow rate of 0.8 mL/min. The samples were detected at 220 nm. Rapid separation was achieved within 7 min. Under the optimized conditions, good linearities of four common nonprotein nitrogen substances were obtained in the range of 0.1-250 mg/L. The detection limits were 9.31 (Cr), 26.19 (Cn), 4.70 (Ua), an 6.30 (Pu) microg/L and the recoveries were in the range of 81%-111% with the relative standar deviations of 0.23%-2.78% (n = 3). The results demonstrate that this method is simple, rapid and accurate with good reproducibility, and can provide early diagnosis and preliminary judgment for type 2 diabetes mellitus (T2DM) patients with renal damage.
authors.affiliation: Key Laboratory of Oil & Gas Fine Chemicals, Ministry of Education, Xinjiang University, Urumqi 830046, China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Key Laboratory of Oil & Gas Fine Chemicals, Ministry of Education, Xinjiang University, Urumqi 830046, China.
authors.forename: Dongqun
authors.initials: D
authors.lastname: Huang
authors.affiliation: Key Laboratory of Oil & Gas Fine Chemicals, Ministry of Education, Xinjiang University, Urumqi 830046, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Nephrology, Xinjiang Uygur Autonomous Region People's Hospital, Urumqi 830001, China.
authors.forename: Shiru
authors.initials: S
authors.lastname: Xu
authors.affiliation: Key Laboratory of Oil & Gas Fine Chemicals, Ministry of Education, Xinjiang University, Urumqi 830046, China.
authors.forename: Shun
authors.initials: S
authors.lastname: Feng
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003401:Creatine; D003404:Creatinine; D003924:Diabetes Mellitus, Type 2 / Q000652:urine; D006801:Humans; D057230:Limit of Detection; D009584:Nitrogen; D011560:Pseudouridine; D015203:Reproducibility of Results; D014527:Uric Acid; D016482:Urinalysis
chemical_list: D011560:Pseudouridine; D014527:Uric Acid; D003404:Creatinine; D003401:Creatine; D009584:Nitrogen