title: Differential ability to resist to complement lysis and invade host cells mediated by MBL in R4 and 860 strains of Trypanosoma cruzi.
abstract: To produce an infection Trypanosoma cruzi must evade lysis by the complement system. During early stages of infection, the lectin pathway plays an important role in host defense and can be activated by binding of mannan-binding lectin (MBL) to carbohydrates on the surface of pathogens. We hypothesized that MBL has a dual role during parasite-host cell interaction as lectin complement pathway activator and as binding molecule to invade the host cell. We used two polarized strains of T. cruzi, R4 (susceptible) and 860 (resistant) strains, to investigate the role of MBL in complement-mediated lysis. Interestingly R4, but not 860 metacyclic strain, markedly increases the invasion of host cells, suggesting that MBL drives the invasion process while the parasite deactivates the Lectin complement pathway.
authors.affiliation: Laboratório de Biologia Molecular de Parasitas e Vetores, Instituto Oswaldo Cruz - Fiocruz., Av Brasil, 4550. Manguinhos-Rio de Janeiro, Brazil.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Evans-Osses
authors.affiliation: Laboratório de Biologia Molecular de Parasitas e Vetores, Instituto Oswaldo Cruz - Fiocruz., Av Brasil, 4550. Manguinhos-Rio de Janeiro, Brazil.
authors.forename: Andres
authors.initials: A
authors.lastname: Mojoli
authors.affiliation: Laboratório de Imunopatologia Molecular, Departamento de Patologia Médica, Universidade Federal do Paraná, Curitiba, Brazil.
authors.forename: Marcia Holsbach
authors.initials: MH
authors.lastname: Beltrame
authors.affiliation: Laboratório de Imunopatologia Molecular, Departamento de Patologia Médica, Universidade Federal do Paraná, Curitiba, Brazil.
authors.forename: Denise Endo
authors.initials: DE
authors.lastname: da Costa
authors.affiliation: Laboratório de Genômica Funcional de Parasitos, Departamento de Bioquimica e Biologia Molecular, Universidade Federal de Parana, Curitiba, Brazil.
authors.forename: Wanderson Duarte
authors.initials: WD
authors.lastname: DaRocha
authors.affiliation: Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany.
authors.forename: Thirumalaisamy P
authors.initials: TP
authors.lastname: Velavan
authors.affiliation: Laboratório de Imunopatologia Molecular, Departamento de Patologia Médica, Universidade Federal do Paraná, Curitiba, Brazil.
authors.forename: Iara
authors.initials: I
authors.lastname: de Messias-Reason
authors.affiliation: Laboratório de Biologia Molecular de Parasitas e Vetores, Instituto Oswaldo Cruz - Fiocruz., Av Brasil, 4550. Manguinhos-Rio de Janeiro, Brazil. Electronic address: marcel.ivan.ramirez@gmail.com.
authors.forename: Marcel Ivan
authors.initials: MI
authors.lastname: Ramirez
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000469:parasitology; D002522:Chlorocebus aethiops; D003167:Complement Activation; D003170:Complement Pathway, Alternative; D006790:Host-Parasite Interactions; D006801:Humans; D007113:Immunity, Innate; D037601:Mannose-Binding Lectin / Q000502:physiology*; D015800:Protozoan Proteins / Q000502:physiology*; D014349:Trypanosoma cruzi / Q000502:physiology*; D014709:Vero Cells
chemical_list: D037601:Mannose-Binding Lectin; D015800:Protozoan Proteins
keywords: Chagas disease; Complement system; Inate immunity; Mannan binding lectin; Parasite–host cell interaction