title: Cell-based splicing of minigenes.
abstract: Cell-based splicing of minigenes is used extensively in the analysis of alternative splicing events. In particular, such assays are critical for identifying or confirming the in vivo relevance of cis- and trans-acting factors in the regulation of particular splicing patterns. Here we provide detailed information on the methods specific to the cell-based analysis of minigene splicing. In addition, we discuss some of the theoretical considerations that must be given to the design of the minigene and subsequent experimental conditions.
authors.affiliation: Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Smith
authors.forename: Kristen W
authors.initials: KW
authors.lastname: Lynch
mesh_terms: D002460:Cell Line; D005091:Exons; D017930:Genes, Reporter; D007438:Introns; D008967:Molecular Biology / Q000379:methods*; D012326:RNA Splicing / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics*; D014162:Transfection
chemical_list: D015534:Trans-Activators