title: Rational design of artificial ON-riboswitches.
abstract: Riboswitches are composed of two regions: one for binding to the ligand (the aptamer domain) and the other for regulating the expression of the gene (the expression platform). In most riboswitches (both natural and artificial), a part of the aptamer domain required for ligand binding is directly involved in the regulation of expression, so that it is difficult to design other ligand-responsive riboswitches based on these riboswitches even by using artificial aptamers obtained through in vitro selection. This chapter describes a method for rationally constructing a foundational ON-riboswitch, which is easily available for the design of other ligand-dependent riboswitches, by introducing a new region (a modulator sequence: MS) in addition to the two basic regions. A facile method for preparing arbitrary molecule-dependent riboswitches based on the foundational riboswitch is also presented.
authors.affiliation: Proteo-Science Center, Ehime University, Matsuyama, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ogawa
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D014176:Protein Biosynthesis; D058928:Riboswitch; D014158:Transcription, Genetic
chemical_list: D052157:Aptamers, Nucleotide; D058928:Riboswitch; D004247:DNA; D008156:Luciferases