title: p53 and PCNA Expression in Keratocystic Odontogenic Tumors Compared with Selected Odontogenic Cysts.
abstract: p53 and PCNA expression in keratocystic odontogenic tumors compared with selected odontogenic cysts Summary: The aim of this study was to evaluate p53 and PCNA expression in different odontogenic lesions regarding their different clinical behaviors. Slices prepared from 94 paraffin-embedded tissue blocks (25 radicular cysts (RC), 23 dentigerous cysts (DC), 23 keratocystic odontogenic tumors (KCOT) and 23 calcifying cystic odontogenic tumors (CCOT)) were stained with p53 and PCNA antibodies using immunohistochemistry procedure. The highest level of p53 expression was in the basal layer of RC, and the highest level of PCNA expression was in the suprabasal layer of KCOT. The differences of p53 expression in basal and suprabasal layers as well as PCNA expression in the suprabasal layer were significant but there was no significant difference in PCNA expression in the basal layer of these lesions. The expression of p53 in the basal layer of RC was higher than in other cysts. This may be due to intensive inflammatory infiltration. Also, the high level of PCNA expression in the suprabasal layer of KCOT may justify its neoplastic nature and tendency to recurrence. KCOT and calcifying cystic odontogenic tumors did not show similar expression of studied biomarkers.
authors.affiliation: Dental Materials Research Center, Dental Faculty, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Seyedmajidi
authors.affiliation: Oral and Maxillofacial Pathology Department, Dental Faculty, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Shima
authors.initials: S
authors.lastname: Nafarzadeh
authors.affiliation: Pathology Department, Medical Faculty, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Sepideh
authors.initials: S
authors.lastname: Siadati
authors.affiliation: Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran.
authors.forename: Shahryar
authors.initials: S
authors.lastname: Shafaee
authors.affiliation: Non-Communicable Pediatrics Diseases Research Center, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Bijani
authors.affiliation: Student Research Committee, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Nazanin
authors.initials: N
authors.lastname: Keshmiri
keywords: PCNA; calcifying cystic odontogenic tumor; dentigerous cyst; keratocystic odontogenic tumor; p53; radicular cyst