title: microRNA-155 acts as an oncogene by targeting the tumor protein 53-induced nuclear protein 1 in esophageal squamous cell carcinoma.
abstract: MicroRNA-155 (miR-155) is overexpressed in many human cancers; however, the function of miR-155 is largely unknown in esophageal squamous cell carcinoma (ESCC). In the present study, we found that miR-155 is dramatically increased in ESCC tissues compared with the paired adjacent normal tissues, which suggested that miR-155 acts as an oncogene in ESCC. We predicted that tumor protein p53-induced nuclear protein 1 (TP53INP1) is a candidate target gene of miR-155 given that miR-155 expression decreased mRNA and protein levels of TP53INP1 as determined by RT-PCR and Western blot analysis. In addition, miR-155 and TP53INP1 showed a negative relation in ESCC tissues. Dual luciferase-based reporter assay indicated direct regulation of TP53INP1 by miR-155. Furthermore, we demonstrated that RNA interference of TP53INP1 increased the proliferation and colonies formation of EC-1 cells. Up-regulation of TP53INP1 abrogated miR-155 induced growth in EC-1 cells and mutation of TP53INP1 in 3'-UTR restored the effects when co-transfected with miR-155. We also indicated that overexpression of miR-155 significantly promoted the proliferation of EC-1 cells in vitro and the development of tumors in nude mice. Taken together, our study reveals that miR-155 acts as an oncogene by targeting TP53INP1 in ESCC.
authors.affiliation: Department of Clinical Laboratory Medicine, Shanghai Tenth People's Hospital of Tongji University Shanghai, China, 200072.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory Medicine, Shanghai Tenth People's Hospital of Tongji University Shanghai, China, 200072.
authors.forename: Chen
authors.initials: C
authors.lastname: Cheng
authors.affiliation: Department of Central Laboratory, Shanghai Tenth People's Hospital of Tongji University Shanghai, China, 200072.
authors.forename: Xiang
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Department of Central Laboratory, Shanghai Tenth People's Hospital of Tongji University Shanghai, China, 200072.
authors.forename: Jiang-Tu
authors.initials: JT
authors.lastname: He
authors.affiliation: Department of Central Laboratory, Shanghai Tenth People's Hospital of Tongji University Shanghai, China, 200072.
authors.forename: Qiu-Hui
authors.initials: QH
authors.lastname: Pan
authors.affiliation: Department of Clinical Laboratory Medicine, Shanghai Tenth People's Hospital of Tongji University Shanghai, China, 200072.
authors.forename: Fen-Yong
authors.initials: FY
authors.lastname: Sun
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004938:Esophageal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009857:Oncogenes; D034622:RNA Interference; D013997:Time Factors; D014162:Transfection; D047368:Tumor Burden; D015854:Up-Regulation
chemical_list: D020413:3' Untranslated Regions; D002352:Carrier Proteins; D006360:Heat-Shock Proteins; C524942:MIRN155 microRNA, human; D035683:MicroRNAs; C429767:TP53INP1 protein, human
keywords: ESCC; TP53INP1; miR-155