title: Bacillus ligniniphilus sp. nov., an alkaliphilic and halotolerant bacterium isolated from sediments of the South China Sea.
abstract: An alkaliphilic and halotolerant Gram-stain-positive bacterium, which was isolated from sediment samples from the South China Sea, was subjected to a taxonomic study. The isolate, strain L1T, grew well at a wide range of temperatures and pH values, 10.0-45.0 °C and pH 6-11, with optima at 30 °C and pH 9.0, respectively. The growth of strain L1T occurred at total salt concentrations of 0-10% (w/v) with an optimum at 2% (w/v). Phylogenetic analysis based on 16S rRNA sequence comparison indicated that the isolate represented a member of the genus Bacillus. The strains most closely related to strain L1T were Bacillus nanhaiisediminis JCM 16507T, Bacillus halodurans DSM 497T and Bacillus pseudofirmus DSM 8715T, with 16S rRNA similarities of 96.5%, 95.9% and 95.7%, respectively. DNA-DNA hybridization of strain L1T with the type strains of the most closely related species, B. nanhaiisediminis JCM 16507T, B. halodurans DSM 497T and B. pseudofirmus DSM 8715T, showed reassociation values of about 21.7%, 14.3% and 13.9%, respectively. The DNA G+C content of strain L1T was 40.76 mol%. The predominant isoprenoid quinone was menaquinone 7 (MK-7). The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The predominant cellular fatty acids of strain L1T were iso-C14 : 0 and anteiso-C15:0. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Based on the phenotypic and phylogenetic characteristics, it is proposed that strain L1T (=JCM 18543T=DSM 26145T) should be classified as the type strain of Bacillus ligniniphilus sp. nov.
authors.affiliation: School of Environmental Engineering, Jiangsu University, 301 Xuefu Road Zhenjiang 212013, PR China.|State Key Laboratory of Dairy Biotechnology, Technology Center of Bright Dairy & Food Co. Ltd, Shanghai 200436, PR China.
authors.forename: Daochen
authors.initials: D
authors.lastname: Zhu
authors.affiliation: Department of Ecosystem Studies, School of Environmental Science, University of Shiga Prefecture, Hikone, Shiga, Japan.
authors.forename: Shoko-Hosoi
authors.initials: SH
authors.lastname: Tanabe
authors.affiliation: School of Environmental Engineering, Jiangsu University, 301 Xuefu Road Zhenjiang 212013, PR China.
authors.forename: Changxiao
authors.initials: C
authors.lastname: Xie
authors.affiliation: Department of Biology, Faculty of Science and Engineering, Konan University, 8-9-1, Okamoto, Higashinada, Kobe, 658-8501, Japan.
authors.forename: Daiske
authors.initials: D
authors.lastname: Honda
authors.affiliation: School of Environmental Engineering, Jiangsu University, 301 Xuefu Road Zhenjiang 212013, PR China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Dairy Biotechnology, Technology Center of Bright Dairy & Food Co. Ltd, Shanghai 200436, PR China.
authors.forename: Lianzhong
authors.initials: L
authors.lastname: Ai
mesh_terms: D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C062629:menaquinone 7