title: Nucleotides as nontoxic endogenous endosomolytic agents in drug delivery.
abstract: Nontoxic endogenous nucleotides such as adenosine triphosphate and guanosine triphosphate have secondary phosphate groups, causing proton-buffering capacity and/or hemolytic activity in endolysosomal pH ranges. Nucleotides co-delivered in single polymeric pDNA nanocarrier induce highly enhanced transfection efficiency with negligible cytotoxicity due to their endosomolytic functions.
authors.affiliation: Department of Pharmacy and Integrated Research, Institute of Pharmaceutical Sciences, College of Pharmacy, The Catholic University of Korea, 43 Jibong-ro, Wonmi-gu, Bucheon-si, Gyeonggi-do, 420-743, Republic of Korea.
authors.forename: Hana
authors.initials: H
authors.lastname: Cho
authors.forename: Yong-Yeon
authors.initials: YY
authors.lastname: Cho
authors.forename: You Han
authors.initials: YH
authors.lastname: Bae
authors.forename: Han Chang
authors.initials: HC
authors.lastname: Kang
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D016503:Drug Delivery Systems / Q000379:methods*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D049329:Nanostructures / Q000737:chemistry* / Q000633:toxicity*; D009711:Nucleotides / Q000737:chemistry* / Q000633:toxicity*; D014162:Transfection
chemical_list: D009711:Nucleotides
keywords: ATP; drug delivery; endosomolytic material; gene delivery; nucleotide