title: Inhibition of fluorescent advanced glycation end products (AGEs) of human serum albumin upon incubation with 3-β-hydroxybutyrate.
abstract: Advanced glycation end products (AGEs), which are the final products of glycation, have a major role in diabetic complication and neurodegenerative disorders. The 3-β-hydroxybutyrate (3BHB), a ketone body which is produced by the liver, can be detected in increased concentrations in individuals post fasting and prolonged exercises and in diabetic (type I) patients. In this study, the inhibitory effect of 3BHB on AGEs formation by glucose from the human serum albumin (HSA) was studied at physiological conditions after 35 days of incubation, using physical techniques such as circular dichroism and fluorescence spectroscopy, as well as differential scanning calorimetry (DSC). The fluorescence intensity measurements of glycated HSA by glucose (GHSA) in the presence of 3BHB indicate a decrease in AGEs formation. The DSC deconvolution profile results also confirm the protective role of 3BHB on incubated with glucose by preventing the enthalpy reduction of the HSA tail segment, compared with the deconvolution profile seen for incubated with glucose alone. The concentration of 3BHB used in this study is in accordance with the concentration detected in the body of individuals post fasting and prolonged exercises.
authors.affiliation: Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Bohlooli
authors.forename: A A
authors.initials: AA
authors.lastname: Moosavi-Movahedi
authors.forename: F
authors.initials: F
authors.lastname: Taghavi
authors.forename: A A
authors.initials: AA
authors.lastname: Saboury
authors.forename: P
authors.initials: P
authors.lastname: Maghami
authors.forename: A
authors.initials: A
authors.lastname: Seyedarabi
authors.forename: F
authors.initials: F
authors.lastname: Moosavi-Movahedi
authors.forename: F
authors.initials: F
authors.lastname: Ahmad
authors.forename: A
authors.initials: A
authors.lastname: Shockravi
authors.forename: M
authors.initials: M
authors.lastname: Habibi-Rezaei
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000008:administration & dosage; D002152:Calorimetry, Differential Scanning; D002942:Circular Dichroism; D048909:Diabetes Complications / Q000378:metabolism* / Q000473:pathology; D015444:Exercise / Q000502:physiology; D005947:Glucose / Q000378:metabolism*; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation / Q000187:drug effects; D006801:Humans; D012709:Serum Albumin / Q000187:drug effects* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
chemical_list: D017127:Glycation End Products, Advanced; D012709:Serum Albumin; D005947:Glucose; D020155:3-Hydroxybutyric Acid