title: Down-regulation of miR-150 induces cell proliferation inhibition and apoptosis in non-small-cell lung cancer by targeting BAK1 in vitro.
abstract: Non-small-cell lung cancer (NSCLC) is one of the most common causes of cancer-related death. Our investigations show that miR-150 is a typical microRNA that is overexpressed in human NSCLC. We characterized the effects of miR-150 overexpression in NSCLC cells and found that down-regulation of miR-150 expression inhibited cell proliferation and induced cell apoptosis in vitro; additionally, up-regulation of miR-150 levels had the opposite effect on tumor growth and progression. Furthermore, we found that the mechanism of the miR-150 effects on NSCLC cells was associated with alterations in the expression of human BRI1-associated receptor kinase 1 (BAK1). miR-150 may function as an oncogene in NSCLC cells by directly targeting BAK1. Thus, these data highlight a novel molecular interaction between miR-150 and BAK1 and provide a novel strategy for NSCLC therapy via the down-regulation of miR-150 expression.
authors.affiliation: Department of Rehabilitation, The 454th Hospital of Chinese PLA, Nanjing, Jiangsu, 210002, China, guxiaoyan05@126.com.
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Gu
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Yi-zhou
authors.initials: YZ
authors.lastname: Luo
authors.forename: Qiang
authors.initials: Q
authors.lastname: Du
authors.forename: Ruo-ran
authors.initials: RR
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Shi
authors.forename: Tian-pei
authors.initials: TP
authors.lastname: Yu
mesh_terms: D017209:Apoptosis; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology*; D049109:Cell Proliferation; D015536:Down-Regulation; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D035683:MicroRNAs / Q000032:analysis / Q000037:antagonists & inhibitors / Q000502:physiology*; D050998:bcl-2 Homologous Antagonist-Killer Protein / Q000037:antagonists & inhibitors* / Q000235:genetics
chemical_list: D035683:MicroRNAs; D050998:bcl-2 Homologous Antagonist-Killer Protein; C494772:BAK1 protein, human; C533097:MIR150, human