title: CD90(+)CD45(-) intraperitoneal mesothelial-like cells inhibit T cell activation by production of arginase I.
abstract: In this study, we analyzed intraperitoneal cells recovered from human samples and found that CD90(+)CD45(-) cells exist as a minor population but vigorously grow in culture, showing the morphological features of mesothelial cells (MC). Interestingly, the MC highly expressed arginase I and markedly suppressed T cell proliferation with the reduction of CD3 ζ chain expression in T cells stimulated by coated anti-CD3 mAb. The addition of nor-NOHA (500 μM), or L-arginine (1 mM) mostly restored the inhibitory effect of MC on T cell proliferation as well as the reduced expression of CD3 ζ chain. The expression level of CD3 ζ chain in T cells in the peritoneal cavity was significantly down-regulated from circulating T cells. These results suggest that intraperitoneal free MC have immunomodulatory functions through the control of L-arginine level, and thus may play significant roles in the pathogenesis of various diseases in the peritoneal cavity.
authors.affiliation: Department of Surgical Oncology, University of Tokyo, Tokyo, Japan. Electronic address: kitayama-1SU@h.u-tokyo.ac.jp.
authors.forename: Joji
authors.initials: J
authors.lastname: Kitayama
authors.affiliation: Department of Surgical Oncology, University of Tokyo, Tokyo, Japan.
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Emoto
authors.affiliation: Department of Surgical Oncology, University of Tokyo, Tokyo, Japan.
authors.forename: Hironori
authors.initials: H
authors.lastname: Yamaguchi
authors.affiliation: Department of Surgical Oncology, University of Tokyo, Tokyo, Japan.
authors.forename: Hironori
authors.initials: H
authors.lastname: Ishigami
authors.affiliation: Department of Surgical Oncology, University of Tokyo, Tokyo, Japan.
authors.forename: Hiroharu
authors.initials: H
authors.lastname: Yamashita
authors.affiliation: Department of Surgical Oncology, University of Tokyo, Tokyo, Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Seto
authors.affiliation: Department of Surgical Oncology, University of Tokyo, Tokyo, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Matsuzaki
authors.affiliation: Department of Surgical Oncology, University of Tokyo, Tokyo, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Watanabe
mesh_terms: D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001119:Arginase / Q000096:biosynthesis* / Q000235:genetics; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D017252:CD3 Complex / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D017493:Leukocyte Common Antigens / Q000172:deficiency / Q000235:genetics*; D008213:Lymphocyte Activation / Q000187:drug effects; D010529:Peritoneal Cavity / Q000166:cytology; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018800:Thy-1 Antigens / Q000235:genetics* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D017252:CD3 Complex; C121704:CD3 antigen, zeta chain; D004791:Enzyme Inhibitors; C404735:N(omega)-hydroxynorarginine; D018800:Thy-1 Antigens; D001120:Arginine; D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human; C574619:ARG1 protein, human; D001119:Arginase
keywords: Arginase; Immunosuppression; Mesothelial cell; Peritoneal cavity; T cell