title: Advances in DNA-directed immobilization.
abstract: DNA-directed immobilization (DDI) of proteins is a chemically mild and highly efficient method for generating (micro)structured patterns of proteins on surfaces. Twenty years after its initial description, the DDI method has proven its robustness and versatility in numerous applications, ranging from biosensing and biomedical diagnostics, to fundamental studies in biology and medicine on the single-cell level. This review gives a brief summary on technical aspects of the DDI method and illustrates its scope for applications with an emphasis on studies in cell biology.
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG-1), Hermann-von-Helmholtz-Platz 1, D-76344 Eggenstein-Leopoldshafen, Germany.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Meyer
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG-1), Hermann-von-Helmholtz-Platz 1, D-76344 Eggenstein-Leopoldshafen, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Giselbrecht
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute for Microstructure Technology (IMT), Hermann-von-Helmholtz-Platz 1, D-76344 Eggenstein-Leopoldshafen, Germany.
authors.forename: Bastian E
authors.initials: BE
authors.lastname: Rapp
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute of Nanotechnology (INT) & Karlsruhe Nano Micro Facility (KNMF), Hermann-von-Helmholtz-Platz 1, D-76344 Eggenstein-Leopoldshafen, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Hirtz
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG-1), Hermann-von-Helmholtz-Platz 1, D-76344 Eggenstein-Leopoldshafen, Germany. Electronic address: niemeyer@kit.edu.
authors.forename: Christof M
authors.initials: CM
authors.lastname: Niemeyer
mesh_terms: D035843:Biomedical Research / Q000379:methods; D015374:Biosensing Techniques / Q000379:methods; D006801:Humans; D061925:Immobilized Nucleic Acids / Q000032:analysis*; D046228:Microarray Analysis / Q000379:methods; D020543:Proteome / Q000032:analysis
chemical_list: D061925:Immobilized Nucleic Acids; D020543:Proteome