title: Single nucleotide polymorphisms of toll-like receptor 7 and toll-like receptor 9 in hepatitis C virus infection patients from central China.
abstract: OBJECTIVE
  To analyze the correlation of polymorphisms of toll-like receptor 7 (TLR7) (rs179009) and toll-like receptor 9 (TLR9) (rs187084) in hepatitis C virus (HCV) infections in the Han population.
  
  
  METHODS
  The genotypes of TLR7IVS2-151 in HCV infection were detected by Sanger sequencing using polymerase chain reaction-restriction fragment length polymorphism to determine the TLR9 T-1486C single nucleotide polymorphisms (SNP) for all enrolled patients.
  
  
  RESULTS
  We found no significant difference between males with spontaneous clearance of HCV versus those chronically infected [χ²=2.71, p=0.10, odd ratios (OR)=0.58, 95% confidence interval (CI) 0.31-1.11]. However, significant differences were found for the distribution of TLR7 (rs179009) in females (χ²=9.46, p=0.01). In females, a significant difference was also found between chronic hepatitis C and those with spontaneous clearance of HCV in terms of TLR7 IVS2-151G/A allele frequencies (χ²=9.50, p=0.00, OR=0.46, 95% CI 0.28-0.75). In HCV-infected patients, no significant association was found between the frequency of TLR9 genotypes and alleles.
  
  
  CONCLUSIONS
  The site of TLR7 IVS2-151 (rs179009) G/A may be a factor for susceptibility of chronic HCV in the female Han population. TLR9T-1486C (rs18084) SNP may not play a major role in HCV infection. However, individual risk profiles for HCV infection did vary by sex and this relationship should be further investigated.
authors.affiliation: Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China. zhangpingan@yahoo.com.cn.
authors.forename: Xin-su
authors.initials: XS
authors.lastname: Wei
authors.forename: Chuan-dong
authors.initials: CD
authors.lastname: Wei
authors.forename: Yong-qing
authors.initials: YQ
authors.lastname: Tong
authors.forename: Cheng-liang
authors.initials: CL
authors.lastname: Zhu
authors.forename: Ping-an
authors.initials: PA
authors.lastname: Zhang
mesh_terms: D044466:Asian People / Q000235:genetics; D002681:China; D016001:Confidence Intervals; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006526:Hepatitis C / Q000235:genetics*; D019698:Hepatitis C, Chronic / Q000235:genetics; D006801:Humans; D008099:Liver / Q000502:physiology; D008297:Male; D020641:Polymorphism, Single Nucleotide; D051199:Toll-Like Receptor 7 / Q000235:genetics*; D051217:Toll-Like Receptor 9 / Q000235:genetics*
chemical_list: C495348:TLR7 protein, human; C495371:TLR9 protein, human; D051199:Toll-Like Receptor 7; D051217:Toll-Like Receptor 9
keywords: Hepatitis C virus; TLR7; TLR9; single nucleotide polymorphism