title: E-cadherin is critical for SC1-induced colony growth of F9 embryonic carcinoma cells.
abstract: BACKGROUND
  Colony morphology of embryonic stem (ES) cells contributes to the maintenance of undifferentiated ES cells. Small molecule 3,4-dihydropy-rimido[4,5-d]pyrimidine (SC1), an inhibitor of ERK1- and RasGAP-dependent signaling pathways, can maintain the compact colony morphology of ES cells. However, information on the influence of SC1 on cell morphological change remains lacking.
  
  
  METHODS
  In this study, mouse ES cells J1 and embryonic carcinoma (EC) cells F9 were cultured in SC1-containing medium to determine the effect of SC1 on cell morphology.
  
  
  RESULTS
  SC1 promotes a more compact morphology of J1 mouse ES cells and induces colony growth of F9 EC cells. Furthermore, the cell adhesion protein E-cadherin is a downstream target of SC1, and E-cadherin is critical for SC1-mediated colony growth of F9 EC cells.
  
  
  CONCLUSIONS
  SC1 maintains and induces compact colony morphology of pluripotent cells, and its downstream target, E-cadherin, is involved in the colony phenotype of F9 EC cells. These results explored the potential role of SC1 in morphological change and gene expression in pluripotent cells.
authors.affiliation: College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.forename: Yongyan
authors.initials: Y
authors.lastname: Wu
authors.forename: Yingying
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Ai
authors.forename: Haibo
authors.initials: H
authors.lastname: Wu
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Shi
authors.forename: Zekun
authors.initials: Z
authors.lastname: Guo
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D051379:Mice; D009363:Neoplasm Proteins / Q000378:metabolism*; D009373:Neoplasms, Germ Cell and Embryonal / Q000378:metabolism* / Q000473:pathology; D014411:Neoplastic Stem Cells / Q000378:metabolism* / Q000473:pathology; D039904:Pluripotent Stem Cells / Q000378:metabolism* / Q000473:pathology; D011720:Pyrazoles / Q000494:pharmacology*; D011743:Pyrimidines / Q000494:pharmacology*; D014410:Tumor Stem Cell Assay
chemical_list: D015820:Cadherins; D009363:Neoplasm Proteins; D011720:Pyrazoles; D011743:Pyrimidines; C515825:SC1 compound