title: Molecular characterization and its antioxidant activity of a newly isolated Streptomyces coelicoflavus BC 01 from mangrove soil.
abstract: OBJECTIVE
  To isolate and identify the biologically active strain of Streptomyces species from mangrove soil of Visakhapatnam region.
  
  
  METHODS
  Actinomycetes are isolated by using starch casein agar media and four potential strains were selected to evaluate the antioxidant activity by using the standard methods DPPH, FRAP and total antioxidant capacity. Further, significant antioxidant activity strain characterized by morphological, physiological, biochemical and molecular characterization.
  
  
  RESULTS
  20 actinomycetes strains were isolated, among them four active isolates designated as BC 01, BC 02, BC 03 and BC 04 were studied for antioxidant activities. Of these four isolates, BC 01 showed a potent antioxidant activity when compared with other isolates. The morphological, biochemical and molecular characterization of the active isolate BC 01 belongs to the genus Streptomyces species. The phylogenetic tree was constructed and nucleotide blast in search indicated that the strain is 99.7% similarity with Streptomyces coelicoflavus.
  
  
  CONCLUSIONS
  The results of the present investigation proven that actinomycetes isolated from mangroves are potent source of antioxidants. The strain BC 01 exhibited a potential in vitro antioxidant activity; studies of actinomycetes from mangrove soil can be useful in discovery of novel species to get novel drugs.
authors.affiliation: Department of Biochemistry, Andhra University, Visakhapatnam 530003, Andhra Pradesh, India.
authors.forename: Kothagorla Venkata
authors.initials: KV
authors.lastname: Raghava Rao
authors.affiliation: Department of Biochemistry, Andhra University, Visakhapatnam 530003, Andhra Pradesh, India.
authors.forename: Tamanam
authors.initials: T
authors.lastname: Raghava Rao
keywords: Actinomycetes; Antioxidants; Molecular characterization; Phylogenetic tree; Streptomyces