title: [Study on a collagenase protocol to extract DNA from remnant feathers in edible bird's nest].
abstract: OBJECTIVE
  To establish a method for extracting genomic DNA from rudimental bird feather from the precious edible bird's nest (EBN) harvested from the swiftlet cave.
  
  
  METHODS
  Observed the EBN using endoscopic and studied the influence of adding collagenase on the extracting yield of DNA. PCR amplification and sequencing for the extraction was also conducted.
  
  
  RESULTS
  Collagenase was used in addition to protease K which could substantively increase the DNA yield. The DNA extracted by this method could be used for PCR and other molecular biology analyses.
  
  
  CONCLUSIONS
  This method can be applied to identify the species types in biological products, especially for animal tissue materials that rich in collagen.
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
authors.forename: Ling-Li
authors.initials: LL
authors.lastname: Wang
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
authors.forename: Nian
authors.initials: N
authors.lastname: Chen
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Zhang
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
authors.forename: Guo-Hong
authors.initials: GH
authors.lastname: Wu
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Lai
mesh_terms: D000818:Animals; D001717:Birds; D017364:Collagenases / Q000378:metabolism*; D004247:DNA / Q000302:isolation & purification*; D005241:Feathers; D023281:Genomics
chemical_list: D004247:DNA; D017364:Collagenases