title: Enhanced biodegradation of alkane hydrocarbons and crude oil by mixed strains and bacterial community analysis.
abstract: In this study, two strains, Acinetobacter sp. XM-02 and Pseudomonas sp. XM-01, were isolated from soil samples polluted by crude oil at Bohai offshore. The former one could degrade alkane hydrocarbons (crude oil and diesel, 1:4 (v/v)) and crude oil efficiently; the latter one failed to grow on alkane hydrocarbons but could produce rhamnolipid (a biosurfactant) with glycerol as sole carbon source. Compared with pure culture, mixed culture of the two strains showed higher capability in degrading alkane hydrocarbons and crude oil of which degradation rate were increased from 89.35 and 74.32 ± 4.09 to 97.41 and 87.29 ± 2.41 %, respectively. In the mixed culture, Acinetobacter sp. XM-02 grew fast with sufficient carbon source and produced intermediates which were subsequently utilized for the growth of Pseudomonas sp. XM-01 and then, rhamnolipid was produced by Pseudomonas sp. XM-01. Till the end of the process, Acinetobacter sp. XM-02 was inhibited by the rapid growth of Pseudomonas sp. XM-01. In addition, alkane hydrocarbon degradation rate of the mixed culture increased by 8.06 to 97.41 % compared with 87.29 % of the pure culture. The surface tension of medium dropping from 73.2 × 10(-3) to 28.6 × 10(-3) N/m. Based on newly found cooperation between the degrader and the coworking strain, rational investigations and optimal strategies to alkane hydrocarbons biodegradation were utilized for enhancing crude oil biodegradation.
authors.affiliation: Department of Environmental Science, School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.forename: Zhengxi
authors.initials: Z
authors.lastname: Zhou
authors.forename: Jianping
authors.initials: J
authors.lastname: Wen
authors.forename: Xueyi
authors.initials: X
authors.lastname: You
authors.forename: Youzhi
authors.initials: Y
authors.lastname: Mao
authors.forename: Chunzhe
authors.initials: C
authors.lastname: Lu
authors.forename: Guangxin
authors.initials: G
authors.lastname: Huo
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Jia
mesh_terms: D000150:Acinetobacter / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000473:Alkanes / Q000737:chemistry / Q000378:metabolism*; D001673:Biodegradation, Environmental; D052918:Environmental Restoration and Remediation / Q000379:methods*; D007700:Kinetics; D008969:Molecular Sequence Data; D010578:Petroleum / Q000378:metabolism*; D010802:Phylogeny; D011549:Pseudomonas / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology
chemical_list: D000473:Alkanes; D010578:Petroleum