title: Lipid constituents of the edible mushroom, Pleurotus giganteus demonstrate anti-Candida activity.
abstract: Different solvent extracts of Pleurotus giganteus fruiting bodies were tested for antifungal activities against Candida species responsible for human infections. The lipids extracted from the ethyl acetate fraction significantly inhibited the growth of all the Candida species tested. Analysis by GC/MS revealed lipid components such as fatty acids, fatty acid methyl esters, ergosterol, and ergosterol derivatives. The sample with high amounts of fatty acid methyl esters was the most effective antifungal agent. The samples were not cytotoxic to a mammalian cell line, mouse embryonic fibroblasts BALB/c 3T3 clone A31. To our knowledge, this is the first report of antifungal activity of the lipid components of Pleurotus giganteus against Candida species.
authors.affiliation: Mushroom Research Centre, Institute of Biological Sciences, Faculty ofScience, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Chia-Wei
authors.initials: CW
authors.lastname: Phan
authors.affiliation: Mushroom Research Centre, Institute of Biological Sciences, Faculty ofScience, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Guan-Serm
authors.initials: GS
authors.lastname: Lee
authors.affiliation: School of Applied Sciences, RMIT University, Victoria 3083, Australia.
authors.forename: Ian G
authors.initials: IG
authors.lastname: Macreadie
authors.affiliation: Mushroom Research Centre, Institute of Biological Sciences, Faculty ofScience, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Sri Nurestri Abd
authors.initials: SN
authors.lastname: Malek
authors.affiliation: Mushroom Research Centre, Institute of Biological Sciences, Faculty ofScience, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: David
authors.initials: D
authors.lastname: Pamela
authors.affiliation: Mushroom Research Centre, Institute of Biological Sciences, Faculty ofScience, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Vikineswary
authors.initials: V
authors.lastname: Sabaratnam
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000032:analysis* / Q000494:pharmacology; D041702:BALB 3T3 Cells; D002175:Candida / Q000187:drug effects*; D008055:Lipids / Q000737:chemistry* / Q000633:toxicity; D051379:Mice; D008826:Microbial Sensitivity Tests; D020076:Pleurotus / Q000737:chemistry*; D018675:Toxicity Tests
chemical_list: D000935:Antifungal Agents; D008055:Lipids