title: Effects of dental adhesives on micronucleus frequency in peripheral blood lymphocytes in vitro.
abstract: Dental adhesives come into direct contact with oral tissues. Due to this close and long-term contact, the materials should exhibit a high degree of biocompatibility. The aim of this study was to evaluate the genotoxic effect of dental adhesives on human lymphocytes in vitro. Polymerized dental adhesives (Excite, Adper Single Bond 2, Prompt L-pop and OptiBond Solo Plus) were eluted in dimethyl sulfoxide for 1 hour, 24 h and 120 h (5 days). Thereafter, lymphocyte cultures were treated with different concentrations of eluates (0.2 microg/mL, 0.5 microg/mL and 5 microg/mL) obtained from each of the tested materials. Genotoxicity was evaluated by micronucleus test. The chi2-test was used on statistical analysis (p < 0.05). After elution period of 1 h, only the highest dose of all tested materials affected the measured cytogenetic parameters. After 24 h, genotoxicity was demonstrated only in cultures treated with eluates in concentrations of 0.5 microg/mL and 5 microg/mL. Based on the results, it is concluded that the use of dental adhesives causes genotoxic effects in human lymphocytes. Toxic effect of these dental adhesives increases with the tested material concentration and decreases with the length of elution period.
authors.affiliation: Novi Zagreb Medical Center Zagreb, Croatia.
authors.forename: Dunja
authors.initials: D
authors.lastname: Prica
authors.affiliation: Department of Endodontics and Restorative Dentistry, Study of Dental Medicine, School of Medicine, University of Split, Split, Croatia.
authors.forename: Antonija
authors.initials: A
authors.lastname: Tadin
authors.affiliation: Department of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Zagreb, Croatia.
authors.forename: Danijela
authors.initials: D
authors.lastname: Marović
authors.affiliation: Department of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Zagreb, Croatia.
authors.forename: Marina
authors.initials: M
authors.lastname: Katunarić
authors.affiliation: Zagreb Dental Polyclinic, Zagreb, Croatia.
authors.forename: Adriana
authors.initials: A
authors.lastname: Prica
authors.affiliation: Department of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Zagreb, Croatia.
authors.forename: Nada
authors.initials: N
authors.lastname: Galić
mesh_terms: D018929:Cell Culture Techniques; D003738:Dental Cements / Q000494:pharmacology*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008422:Materials Testing; D008689:Methacrylates / Q000494:pharmacology*; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects; D015162:Micronucleus Tests; D019279:Resin Cements / Q000494:pharmacology*
chemical_list: C554641:Adper single bond 2; D003738:Dental Cements; C420130:Excite cement; D008689:Methacrylates; C451600:OptiBond Solo Plus; C408584:Prompt L-Pop; D019279:Resin Cements