title: Compositional shift in lipid fractions during lipid accumulation and turnover in Schizochytrium sp.
abstract: Single cell oils (SCOs), a complex lipid system, contains neutral lipids (NLs), polar lipids (PLs) and unsaponifiable matters (UMs). To investigate the dynamic changes and the metabolic competition mechanism of different components of SCOs, changes in lipid composition of Schizochytrium sp. were monitored in lipid accumulation and turnover stages. Lipid content could reach 69.98% in biomass during the lipid accumulation stage, while, after the exhaustion of glucose, the content decreased to 45.51% and 20.6g/L non-oil biomass was synthesis. Polyunsaturated fatty acids (PUFAs) were easier to bind with PLs. NLs were preferentially converted to PLs during lipid turnover stage, accompanied by the degradation of saturated fatty acids and the increase of UMs. Meanwhile, a positive correlation between the synthesis of PUFAs and unsaponifiable matters exited in Schizochytrium sp., and increasing the content of UMs from 45 to 100mg/L could increase the PUFA percentage from 64% to 74% effectively.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing 210009, People's Republic of China.
authors.forename: Lu-Jing
authors.initials: LJ
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing 210009, People's Republic of China.
authors.forename: Guan-Nan
authors.initials: GN
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing 210009, People's Republic of China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Ji
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing 210009, People's Republic of China.
authors.forename: Xue-Chao
authors.initials: XC
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing 210009, People's Republic of China. Electronic address: biotech@njut.edu.cn.
authors.forename: He
authors.initials: H
authors.lastname: Huang
mesh_terms: D061249:Batch Cell Culture Techniques; D005591:Chemical Fractionation; D002784:Cholesterol / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D050356:Lipid Metabolism / Q000187:drug effects; D013185:Squalene / Q000378:metabolism; D058009:Stramenopiles / Q000254:growth & development / Q000378:metabolism*; D019207:beta Carotene / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D019207:beta Carotene; D013185:Squalene; D002784:Cholesterol; D005947:Glucose
keywords: Docosahexaenoic acid; Lipid accumulation; Schizochytrium sp.; Turnover