title: Effect of spleen lymphocytes on the splenomegaly in hepatocellular carcinoma-bearing mice.
abstract: OBJECTIVE
  To study the effect of spleen lymphocytes on the splenomegaly by hepatocellular carcinoma-bearing mouse model.
  
  
  METHODS
  Cell counts, cell cycle distribution, the percentage of lymphocytes subsets and the levels of IL-2 were measured, and two-dimensional gel electrophoresis (2-DE) was used to investigate the relationship between spleen lymphocytes and splenomegaly in hepatocellular carcinoma-bearing mice.
  
  
  RESULTS
  Compared with the normal group, the thymus was obviously atrophied and the spleen was significantly enlarged in the tumor-bearing group. Correlation study showed that the number of whole spleen cells was positively correlated with the splenic index. The cell diameter and cell-cycle phase distribution of splenocytes in the tumor-bearing group showed no significant difference compared to the normal group. The percentage of CD3+ T lymphocytes and CD8+ T lymphocytes in spleen and peripheral blood of tumor-bearing mice were substantially higher than that in the normal mice. Meanwhile, the IL-2 level was also higher in the tumor-bearing group than in the normal group. Furthermore, two dysregulated protein, β-actin and S100-A9 were identiﬁed in spleen lymphocytes from H22-bearing mice, which were closely related to cellular motility.
  
  
  CONCLUSIONS
  It is suggested that dysregulated β-actin and S100-A9 can result in recirculating T lymphocytes trapped in the spleen, which may explain the underlying cause of splenomegaly in H22-bearing mice.
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China.
authors.forename: Jing Jing
authors.initials: JJ
authors.lastname: Fang
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China.
authors.forename: Zhen Yuan
authors.initials: ZY
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Dong
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China.
authors.forename: Guo Qiang
authors.initials: GQ
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China.
authors.forename: An Guo
authors.initials: AG
authors.lastname: Teng
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China.
authors.forename: An Jun
authors.initials: AJ
authors.lastname: Liu
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000150:complications*; D002453:Cell Cycle; D005260:Female; D008113:Liver Neoplasms / Q000150:complications*; D008214:Lymphocytes / Q000502:physiology; D051379:Mice; D008813:Mice, Inbred ICR; D009374:Neoplasms, Experimental / Q000628:therapy; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013154:Spleen / Q000166:cytology* / Q000473:pathology; D013163:Splenomegaly / Q000209:etiology / Q000628:therapy*; D013950:Thymus Gland
keywords: Hepatocellular carcinoma; Lymphocytes; S100-A9; Splenomegaly; β-actin