title: Specificity protein 1 expression contributes to Bcl-w-induced aggressiveness in glioblastoma multiforme.
abstract: We already had reported that Bcl-w promotes invasion or migration in gastric cancer cells and glioblastoma multiforme (GBM) by activating matrix metalloproteinase-2 (MMP-2) via specificity protein 1 (Sp1) or β-cateinin, respectively. High expression of Bcl-w also has been reported in GBM which is the most common malignant brain tumor and exhibits aggressive and invasive behavior. These reports propose that Bcl-w-induced signaling is strongly associated with aggressive characteristic of GBM. We demonstrated that Sp1 protein or mRNA expression is induced by Bcl-w using Western blotting or RT-PCR, respectively, and markedly elevated in high-grade glioma specimens compared with low-grade glioma tissues using tissue array. However, relationship between Bcl-w-related signaling and aggressive characteristic of GBM is poorly characterized. This study suggested that Bcl-w-induced Sp1 activation promoted expression of glioma stem-like cell markers, such as Musashi, Nanog, Oct4 and sox-2, as well as neurosphere formation and invasiveness, using western blotting, neurosphere formation assay, or invasion assay, culminating in their aggressive behavior. Therefore, Bcl-w-induced Sp1 activation is proposed as a putative marker for aggressiveness of GBM.
authors.affiliation: Division of Radiation Cancer Research, Korea Institute of Radiological & Medical Sciences, Seoul 139-706, Korea.
authors.forename: Woo Sang
authors.initials: WS
authors.lastname: Lee
authors.forename: Junhye
authors.initials: J
authors.lastname: Kwon
authors.forename: Dong Ho
authors.initials: DH
authors.lastname: Yun
authors.forename: Young Nam
authors.initials: YN
authors.lastname: Lee
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Woo
authors.forename: Myung-Jin
authors.initials: MJ
authors.lastname: Park
authors.forename: Jae-Seon
authors.initials: JS
authors.lastname: Lee
authors.forename: Young-Hoon
authors.initials: YH
authors.lastname: Han
authors.forename: In Hwa
authors.initials: IH
authors.lastname: Bae
mesh_terms: D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D004789:Enzyme Activation; D005909:Glioblastoma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D014411:Neoplastic Stem Cells / Q000502:physiology*; D016329:Sp1 Transcription Factor / Q000378:metabolism*; D018874:Spheroids, Cellular / Q000378:metabolism
chemical_list: D051017:Apoptosis Regulatory Proteins; C491073:BCL2L2 protein, human; D016329:Sp1 Transcription Factor; C108209:SP1 protein, human; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2