title: Bacteriophage therapy of a Vibrio parahaemolyticus infection caused by a multiple-antibiotic-resistant O3:K6 pandemic clinical strain.
abstract: BACKGROUND
  Recently isolated Vibrio parahaemolyticus strains have displayed multiple antibiotic resistance. Alternatives to conventional antibiotics are needed, especially for the multiple-antibiotic-resistant V. parahaemolyticus pandemic strain.
  
  
  METHODS
  A bacteriophage, designated pVp-1, showed effective infectivity for multiple-antibiotic-resistant V. parahaemolyticus and V. vulnificus, including V. parahaemolyticus pandemic strains. The therapeutic potential of the phage was studied in a mouse model of experimental infection using a multiple-antibiotic-resistant V. parahaemolyticus pandemic strain. We monitored the survivability and histopathological changes, quantified the bacterial and phage titers during phage therapy, and observed the immune response induced by phage induction.
  
  
  RESULTS
  Phage-treated mice displayed protection from a V. parahaemolyticus infection and survived lethal oral and intraperitoneal bacterial challenges.
  
  
  CONCLUSIONS
  To the best of our knowledge, this is the first report of phage therapy in a mouse model against a multiple-antibiotic–resistant V. parahaemolyticus pandemic strain infection.
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Jun
authors.forename: Tae Hoon
authors.initials: TH
authors.lastname: Shin
authors.forename: Ji Hyung
authors.initials: JH
authors.lastname: Kim
authors.forename: Sang Phil
authors.initials: SP
authors.lastname: Shin
authors.forename: Jee Eun
authors.initials: JE
authors.lastname: Han
authors.forename: Gang Joon
authors.initials: GJ
authors.lastname: Heo
authors.forename: Mahanama
authors.initials: M
authors.lastname: De Zoysa
authors.forename: Gee Wook
authors.initials: GW
authors.lastname: Shin
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Chai
authors.forename: Se Chang
authors.initials: SC
authors.lastname: Park
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000254:growth & development*; D001691:Biological Therapy / Q000379:methods*; D004195:Disease Models, Animal; D024901:Drug Resistance, Multiple, Bacterial; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D016019:Survival Analysis; D016896:Treatment Outcome; D014735:Vibrio Infections / Q000382:microbiology* / Q000628:therapy*; D014736:Vibrio parahaemolyticus / Q000187:drug effects* / Q000821:virology*