title: [Effects of drynariae rhizoma water-extraction on the ability of osteogenic differentiation and it's mechanism].
abstract: OBJECTIVE
  To investigate the effects of Drynariae Rhizoma water-extraction on the ability of osteogenic differentiation and the expression of TGF-beta1 and BMP-2 in inducing mesenchymal stem cells (MSCs) differentiation into osteoblast and its mechanism.
  
  
  METHODS
  MSCs were isolated and purified by differential time adherent method; The most effective concentration of the Drynariae Rhizoma water-extraction on the proliferation and ostogenic differentiation of MSCs was confirmed by MTT method and the activity and positive staining of alkaline phosphatase (ALP) respectively; According to different induced condition, MSCs were divided into 4 groups: control group, classic group (induced by the classic osteoblast-induced system), Drynariae Rhizoma water-extraction group (induced by the most effective concentration of Drynariae Rhizoma water-extraction on differentiation into osteoblast) and Drynariae Rhizoma water-extraction + classic group (induced by the combination of classic osteoblast-induced system and the most effective concentration of Drynariae Rhizoma water-extraction on differentiation into osteoblast). ALP,type I collagen (Col I), bone gla protein (BGP) and calcium nodes in each group were detected and compared to indicate the osteogenic differentiation of each group. TGF-beta1 and BMP-2 were detected by ELISA.
  
  
  RESULTS
  The most effective concentration of the Drynariae Rhizoma water-extraction on the proliferation and osteogenic differentiation of MSCs was 5 microg/mL and 50 microg/mL, respectively. The classic group, Drynariae Rhizoma water-extraction group and Drynariae Rhizoma water-extraction + classic group could promote the osteogenic differentiation of MSCs and increase the expression of TGF-beta1 and BMP-2.
  
  
  CONCLUSIONS
  The Drynariae Rhizoma water-extraction can promote the proliferation and osteogenic differentiation of MSCs. Epimedium water-extraction can improve the differentiation of MSC into osteoblast and its mechanism may be related to increasing the expression of TGF-beta1 and BMP-2.
authors.affiliation: Pharmacy College of Jinan University, Guangzhou 510632, China. doctormonkey@126.com
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.affiliation: The first Affiliated Hospital of Jinan University, Guangzhou 510630, China.
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Zhu
authors.affiliation: The first Affiliated Hospital of Jinan University, Guangzhou 510630, China.
authors.forename: Pan-Pan
authors.initials: PP
authors.lastname: Wang
authors.affiliation: Pharmacy College of Jinan University, Guangzhou 510632, China.
authors.forename: Rong-Hua
authors.initials: RH
authors.lastname: Zhang
mesh_terms: D000469:Alkaline Phosphatase; D055396:Bone Morphogenetic Protein 2; D002454:Cell Differentiation; D024042:Collagen Type I; D059630:Mesenchymal Stem Cells; D010006:Osteoblasts; D010012:Osteogenesis; D029621:Polypodiaceae; D027343:Rhizome; D053773:Transforming Growth Factor beta1; D014867:Water
chemical_list: D055396:Bone Morphogenetic Protein 2; D024042:Collagen Type I; D053773:Transforming Growth Factor beta1; D014867:Water; D000469:Alkaline Phosphatase