title: HIF-1-dependent induction of Jumonji domain-containing protein (JMJD) 3 under hypoxic conditions.
abstract: Jumonji domain-containing proteins (JMJD) catalyze the oxidative demethylation of a methylated lysine residue of histones by using O2, α-ketoglutarate, vitamin C, and Fe(II). Several JMJDs are induced by hypoxic stress to compensate their presumed reduction in catalytic activity under hypoxia. In this study, we showed that an H3K27me3 specific histone demethylase, JMJD3 was induced by hypoxia-inducible factor (HIF)-1α/β under hypoxia and that treatment with Clioquinol, a HIF-1α activator, increased JMJD3 expression even under normoxia. Chromatin immunoprecipitation (ChIP) analyses showed that both HIF-1α and its dimerization partner HIF-1β/Arnt occupied the first intron region of the mouse JMJD3 gene, whereas the HIF-1α/β heterodimer bound to the upstream region of the human JMJD3, indicating that human and mouse JMJD3 have hypoxia-responsive regulatory regions in different locations. This study shows that both mouse and human JMJD3 are induced by HIF-1.
authors.affiliation: Department of Life Science, University of Seoul, Seoul 130-743, Korea.
authors.forename: Ho-Youl
authors.initials: HY
authors.lastname: Lee
authors.forename: Kang
authors.initials: K
authors.lastname: Choi
authors.forename: Hookeun
authors.initials: H
authors.lastname: Oh
authors.forename: Young-Kwon
authors.initials: YK
authors.lastname: Park
authors.forename: Hyunsung
authors.initials: H
authors.lastname: Park
mesh_terms: D041721:3T3-L1 Cells; D053687:Adult Stem Cells / Q000378:metabolism; D000818:Animals; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator / Q000502:physiology*; D001483:Base Sequence; D015687:Cell Hypoxia; D002478:Cells, Cultured; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000502:physiology*; D056484:Jumonji Domain-Containing Histone Demethylases / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D020218:Response Elements; D013045:Species Specificity; D015533:Transcriptional Activation
chemical_list: C497397:Arnt protein, mouse; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator; D056484:Jumonji Domain-Containing Histone Demethylases; C524220:KDM6B protein, human