title: Biophysical features of MagA expression in mammalian cells: implications for MRI contrast.
abstract: We compared overexpression of the magnetotactic bacterial gene MagA with the modified mammalian ferritin genes HF + LF, in which both heavy and light subunits lack iron response elements. Whereas both expression systems have been proposed for use in non-invasive, magnetic resonance (MR) reporter gene expression, limited information is available regarding their relative potential for providing gene-based contrast. Measurements of MR relaxation rates in these expression systems are important for optimizing cell detection and specificity, for developing quantification methods, and for refinement of gene-based iron contrast using magnetosome associated genes. We measured the total transverse relaxation rate (R2*), its irreversible and reversible components (R2 and R2', respectively) and the longitudinal relaxation rate (R1) in MDA-MB-435 tumor cells. Clonal lines overexpressing MagA and HF + LF were cultured in the presence and absence of iron supplementation, and mounted in a spherical phantom for relaxation mapping at 3 Tesla. In addition to MR measures, cellular changes in iron and zinc were evaluated by inductively coupled plasma mass spectrometry, in ATP by luciferase bioluminescence and in transferrin receptor by Western blot. Only transverse relaxation rates were significantly higher in iron-supplemented, MagA- and HF + LF-expressing cells compared to non-supplemented cells and the parental control. R2* provided the greatest absolute difference and R2' showed the greatest relative difference, consistent with the notion that R2' may be a more specific indicator of iron-based contrast than R2, as observed in brain tissue. Iron supplementation of MagA- and HF + LF-expressing cells increased the iron/zinc ratio approximately 20-fold, while transferrin receptor expression decreased approximately 10-fold. Level of ATP was similar across all cell types and culture conditions. These results highlight the potential of magnetotactic bacterial gene expression for improving MR contrast.
authors.affiliation: Imaging Program, Lawson Health Research Institute London, ON, Canada ; Medical Biophysics, Western University London, ON, Canada ; Collaborative Graduate Program in Molecular Imaging, Western University London, ON, Canada.
authors.forename: Anindita
authors.initials: A
authors.lastname: Sengupta
authors.affiliation: Imaging Program, Lawson Health Research Institute London, ON, Canada ; Medical Biophysics, Western University London, ON, Canada ; Collaborative Graduate Program in Molecular Imaging, Western University London, ON, Canada.
authors.forename: Karina
authors.initials: K
authors.lastname: Quiaoit
authors.affiliation: Imaging Program, Lawson Health Research Institute London, ON, Canada ; Medical Biophysics, Western University London, ON, Canada.
authors.forename: R Terry
authors.initials: RT
authors.lastname: Thompson
authors.affiliation: Imaging Program, Lawson Health Research Institute London, ON, Canada ; Medical Biophysics, Western University London, ON, Canada.
authors.forename: Frank S
authors.initials: FS
authors.lastname: Prato
authors.affiliation: Imaging Program, Lawson Health Research Institute London, ON, Canada ; Medical Biophysics, Western University London, ON, Canada.
authors.forename: Neil
authors.initials: N
authors.lastname: Gelman
authors.affiliation: Imaging Program, Lawson Health Research Institute London, ON, Canada ; Medical Biophysics, Western University London, ON, Canada ; Collaborative Graduate Program in Molecular Imaging, Western University London, ON, Canada.
authors.forename: Donna E
authors.initials: DE
authors.lastname: Goldhawk
keywords: MagA; cancer cells; iron; magnetic resonance imaging; modified ferritin subunits; relaxation rates