title: Histone H3K56 acetylation is required for quelling-induced small RNA production through its role in homologous recombination.
abstract: Quelling and DNA damage-induced small RNA (qiRNA) production are RNA interference (RNAi)-related phenomenon from repetitive genomic loci in Neurospora. We have recently proposed that homologous recombination from repetitive DNA loci allows the RNAi pathway to recognize repetitive DNA to produce small RNA. However, the mechanistic detail of this pathway remains largely unclear. By systematically screening the Neurospora knock-out library, we identified RTT109 as a novel component required for small RNA production. RTT109 is a histone acetyltransferase for histone H3 lysine 56 (H3K56) and H3K56 acetylation is essential for the small RNA biogenesis pathway. Furthermore, we showed that RTT109 is required for homologous recombination and H3K56Ac is enriched around double strand break, which overlaps with RAD51 binding. Taken together, our results suggest that H3K56 acetylation is required for small RNA production through its role in homologous recombination.
authors.affiliation: From the Department of Physiology, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Zhang
authors.forename: Qiuying
authors.initials: Q
authors.lastname: Yang
authors.forename: Guangyan
authors.initials: G
authors.lastname: Sun
authors.forename: She
authors.initials: S
authors.lastname: Chen
authors.forename: Qun
authors.initials: Q
authors.lastname: He
authors.forename: Shaojie
authors.initials: S
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000107:Acetylation; D055786:Gene Knockout Techniques; D051548:Histone Acetyltransferases / Q000737:chemistry / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D006657:Histones / Q000737:chemistry* / Q000378:metabolism*; D059765:Homologous Recombination / Q000235:genetics*; D008239:Lysine / Q000378:metabolism*; D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000096:biosynthesis* / Q000235:genetics*
chemical_list: D006657:Histones; D034741:RNA, Small Interfering; D051548:Histone Acetyltransferases; D008239:Lysine
keywords: Homologous Recombination; Neurospora; RNA Interference (RNAi); RNA Silencing; siRNA