title: Global protein-RNA interaction mapping at single nucleotide resolution by iCLIP-seq.
abstract: Eukaryotic genomes encode a large number of RNA-binding proteins, which play critical roles in many aspects of gene regulation. To functionally characterize these proteins, a key step is to map their interactions with target RNAs. UV crosslinking and immunoprecipitation coupled with high-throughput sequencing has become the standard method for this purpose. Here we describe the detailed procedure that we have used to characterize the protein-RNA interactions of the mRNA 3' processing factors.
authors.affiliation: Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, CA, USA.
authors.forename: Chengguo
authors.initials: C
authors.lastname: Yao
authors.forename: Lingjie
authors.initials: L
authors.lastname: Weng
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Shi
mesh_terms: D001665:Binding Sites; D056890:Eukaryota; D016678:Genome; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D047468:Immunoprecipitation / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*; D012313:RNA / Q000235:genetics* / Q000302:isolation & purification; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D016601:RNA-Binding Proteins; D012313:RNA