title: 2,3,7,8-Tetrachlorodibenzo-p-dioxin Delays Palatal Shelf Elevation and Suppresses Wnt5a and Lymphoid Enhancing-Binding Factor 1 Signaling in Developing Palate.
abstract: OBJECTIVE
  2,3,7,8-Tetrachlorodibenzo-p-dioxin contributes to cleft palate, but the cellular and molecular mechanisms responsible for the deleterious effect on the developing palate are unclear. Because Wnt signaling is associated with 2,3,7,8-tetrachlorodibenzo-p-dioxin in organ development, we wondered whether the malformation of the palate also results from altered Wnt signaling.
  
  
  RESULTS
  The 2,3,7,8-tetrachlorodibenzo-p-dioxin administration affected cell proliferation of the anteroposterior axis of the palatal shelf and delayed shelf elevation in mice. The activity of Wnt5a and lymphoid enhancing-binding factor 1 was inhibited by 2,3,7,8-tetrachlorodibenzo-p-dioxin in the developing palate.
  
  
  CONCLUSIONS
  Downregulated Wnt5a and lymphoid enhancing-binding factor 1 are associated with 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced cleft palate. Moreover, delayed shelf elevation by 2,3,7,8-tetrachlorodibenzo-p-dioxin is the crucial mechanism contributing to the high incidence of cleft palate. Our findings may help in elucidating the mechanisms of 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced cleft palate.
authors.forename: Xiao
authors.initials: X
authors.lastname: Hu
authors.forename: Jian Hua
authors.initials: JH
authors.lastname: Gao
authors.forename: Yun Jun
authors.initials: YJ
authors.lastname: Liao
authors.forename: Shi Jie
authors.initials: SJ
authors.lastname: Tang
authors.forename: Feng
authors.initials: F
authors.lastname: Lu
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002972:Cleft Palate / Q000139:chemically induced* / Q000196:embryology* / Q000473:pathology; D020287:In Situ Nick-End Labeling; D051821:Lymphoid Enhancer-Binding Factor 1 / Q000187:drug effects*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D060449:Wnt Signaling Pathway / Q000187:drug effects*
chemical_list: D051821:Lymphoid Enhancer-Binding Factor 1; D000072317:Polychlorinated Dibenzodioxins
keywords: 2,3,7,8-tetrachlorodibenzo-p-dioxin; Lef-1; Wnt5a; cleft palate