title: Construction of artificial biosynthetic pathways for resveratrol glucoside derivatives.
abstract: Resveratrol, which is a polyphenolic antioxidant, is dose-dependent when used to provide health benefits, to enhance stress resistance, and to extend lifespans. However, even though resveratrol has therapeutic benefits, its clinical therapeutic effect is limited owing to its low oral bioavailability. An Escherichia coli system was developed that contains an artificial biosynthetic pathway that produces resveratrol glucoside derivatives, such as resveratrol-3-Oglucoside (piceid) and resveratrol-4'-O-glucoside (resveratroloside), from simple carbon sources. This artificial biosynthetic pathway contains a glycosyltransferase addition (YjiC from Bacillus) with resveratrol biosynthetic genes. The produced glucoside compounds were verified through the presence of a product peak(s) and also through LC/MS analyses. The strategy used in this research demonstrates the first harnessing of E. coli for de novo synthesis of resveratrol glucoside derivatives from a simple sugar medium.
authors.affiliation: Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology, Ochang-eup 363-883, Republic of Korea.
authors.forename: Oksik
authors.initials: O
authors.lastname: Choi
authors.forename: Jae Kyoung
authors.initials: JK
authors.lastname: Lee
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Kang
authors.forename: Ramesh Prasad
authors.initials: RP
authors.lastname: Pandey
authors.forename: Jae-Kyung
authors.initials: JK
authors.lastname: Sohng
authors.forename: Jong Seog
authors.initials: JS
authors.lastname: Ahn
authors.forename: Young-Soo
authors.initials: YS
authors.lastname: Hong
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism*; D001426:Bacterial Proteins; D053898:Biosynthetic Pathways; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D023061:Gene Order; D005822:Genetic Vectors / Q000235:genetics; D005960:Glucosides / Q000737:chemistry / Q000378:metabolism*; D000077185:Resveratrol; D013267:Stilbenes / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D001426:Bacterial Proteins; D005960:Glucosides; D013267:Stilbenes; D000077185:Resveratrol