title: Genotypic to expression profiling of bovine calcium channel, voltage-dependent, alpha-2/delta subunit 1 gene, and their association with bovine mastitis among Frieswal (HFX Sahiwal) crossbred cattle of Indian origin.
abstract: Calcium channel, voltage-dependent, alpha-2/delta subunit 1 (CACNA2D1) gene is considered to be an important noncytokine candidate gene influencing mastitis. Scanty of reports are available until today regarding the role play of CACNA2D1 gene on the susceptibility of bovine mastitis. We interrogated the CACNA2D1 G519663A [A>G] SNP by PCR-RFLP among two hundreds Frieswal (HF X Sahiwal) crossbred cattle of Indian origin. Genotypic frequency of AA (51.5, n=101) was comparatively higher than AG (35, n=70) and GG (14.5, n=29). Association of Somatic cell score (SCS) with genotypes revealed that, GG genotypes showing lesser count (less susceptible to mastitis) compare to AA and AG. Relative expression of CACNA2D1 transcript (in milk samples) was significantly higher among GG than AG and AA. Further we have also isolated blood sample from the all groups and PBMCs were cultured from each blood sample as per the standard protocol. They were treated with Calcium channel blocker and the expression level of the CACNA2D1 gene was evaluated by Real Time PCR. Results show that expression level decline in each genotypic group after treatment and expression level of GG are again significantly higher than AA and AG. Thus, it may be concluded that GG genotypic animals are favorable for selecting disease resistant breeds.
authors.affiliation: a Molecular Genetics Laboratory, Project Directorate on Cattle, Indian Council of Agricultural Research, Meerut Cantt , Uttar Pradesh , India.
authors.forename: Rajib
authors.initials: R
authors.lastname: Deb
authors.forename: Umesh
authors.initials: U
authors.lastname: Singh
authors.forename: Sushil
authors.initials: S
authors.lastname: Kumar
authors.forename: Arun
authors.initials: A
authors.lastname: Kumar
authors.forename: Rani
authors.initials: R
authors.lastname: Singh
authors.forename: Gyanendra
authors.initials: G
authors.lastname: Sengar
authors.forename: Sandeep
authors.initials: S
authors.lastname: Mann
authors.forename: Arjava
authors.initials: A
authors.lastname: Sharma
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000235:genetics*; D002417:Cattle / Q000145:classification / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006824:Hybridization, Genetic / Q000235:genetics; D007194:India; D008414:Mastitis, Bovine / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D013045:Species Specificity
chemical_list: D015220:Calcium Channels