title: Rapid and sensitive UPLC-MS/MS method for the determination of domperidone in human plasma and its application to pharmacokinetic study.
abstract: In this study, a simple, rapid and sensitive ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method is described for determination of domperidone in human plasma samples using oxcarbazepine as the internal standard (IS). Sample preparation was accomplished through protein precipitation with methanol, and chromatographic separation was performed on an Acquity BEH C18 column (2.1 mm×50 mm, 1.7 μm) with gradient profile at a flow of 0.45 mL/min. Mass spectrometric analysis was performed using a QTrap5500 mass spectrometer coupled with an electro-spray ionization (ESI) source in the positive ion mode. The MRM transition of m/z 426.3→175.2 was used to quantify for domperidone. The linearity of this method was found to be within the concentration range of 0.25-100.0 ng/mL for domperidone in human plasma. Only 1.5 min was needed for an analytical run. The method herein described was superior to previous methods and was successfully applied to the pharmacokinetic study of domperidone in healthy Chinese volunteers after oral administration.
authors.affiliation: Department of Pharmacology, Medical College of Henan University of Science and Technology, Luoyang, China.
authors.forename: X-J
authors.initials: XJ
authors.lastname: Qiu
authors.affiliation: The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
authors.forename: S-L
authors.initials: SL
authors.lastname: Zheng
authors.affiliation: Department of Pharmacology, Medical College of Henan University of Science and Technology, Luoyang, China.
authors.forename: Y-F
authors.initials: YF
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, Medical College of Henan University of Science and Technology, Luoyang, China.
authors.forename: R
authors.initials: R
authors.lastname: Wang
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
authors.forename: L
authors.initials: L
authors.lastname: Ye
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004294:Domperidone / Q000097:blood* / Q000493:pharmacokinetics; D004355:Drug Stability; D006801:Humans; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004294:Domperidone