title: Clinicopathological roles of S100A8 and S100A9 in cutaneous squamous cell carcinoma in vivo and in vitro.
abstract: S100A8 and S100A9 are members of the S100 protein family and exist in neutrophils, monocytes, and macrophages. Recent studies have shown that S100A8 and S100A9 are associated with various neoplastic disorders; however, their roles in cutaneous squamous cell carcinoma (SCC) are not well defined. To investigate the expression and function of S100A8 and S100A9 in skin tumors, we examined the expression levels of S100A8 and S100A9 between premalignant and malignant skin tumors and investigated the functional roles of S100A8 and S100A9 in vitro and in vivo using recombinant adenovirus expressing S100A8 or S100A9. The immunopositive staining rates and intensities of S100A8 and S100A9 were higher in SCC than in premalignant skin tumors. When S100A8 and/or S100A9 were overexpressed in SCC12 cells using a recombinant adenovirus, cell growth and motility were increased. Similarly, when mouse skin was intradermally injected with SCC12 cells overexpressing S100A8 and/or S100A9, there were remarkable increases in tumor growth and volume. Both S100A8 and S100A9 are highly expressed in cutaneous SCC and play important roles in tumorigenesis. We suggest that S100A8 and S100A9 may be potential therapeutic targets for the prevention or treatment of SCC in skin.
authors.affiliation: Department of Dermatology and Research Institute for Medical Sciences, Chungnam National University, Daejeon, Korea.
authors.forename: Dae-Kyoung
authors.initials: DK
authors.lastname: Choi
authors.forename: Zheng Jun
authors.initials: ZJ
authors.lastname: Li
authors.forename: In-Kyu
authors.initials: IK
authors.lastname: Chang
authors.forename: Min-Kyung
authors.initials: MK
authors.lastname: Yeo
authors.forename: Jin-Man
authors.initials: JM
authors.lastname: Kim
authors.forename: Kyung-Cheol
authors.initials: KC
authors.lastname: Sohn
authors.forename: Myung
authors.initials: M
authors.lastname: Im
authors.forename: Young-Joon
authors.initials: YJ
authors.lastname: Seo
authors.forename: Jeung-Hoon
authors.initials: JH
authors.lastname: Lee
authors.forename: Chang-Deok
authors.initials: CD
authors.lastname: Kim
authors.forename: Young
authors.initials: Y
authors.lastname: Lee
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D040501:Calgranulin A / Q000096:biosynthesis* / Q000235:genetics; D040502:Calgranulin B / Q000096:biosynthesis* / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015870:Gene Expression; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D012878:Skin Neoplasms / Q000473:pathology*; D014183:Transplantation, Heterologous; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: D040501:Calgranulin A; D040502:Calgranulin B