title: Effects of subchronic samarium exposure on the histopathological structure and apoptosis regulation in mouse testis.
abstract: To evaluate the reproductive toxicity of samarium, a widely used rare earth element, male ICR mice were orally exposed to samarium nitrate for 90 days for lesion evaluation in the testis. Decreased organ coefficients, disorganized seminiferous tubules, and decreased spermatogenic cells and sperm of the testis were observed extensively in the treated groups, indicating that the testis is a target organ of samarium. Electron microscopy confirmed that the lesions inside the spermatogenic cells and sperm mainly included mitochondrial swelling, mitochondrial vacuolization, fuzzy nuclear membranes, and marginated chromatin. Increased spermatogenic cell apoptosis rate in the testis was confirmed with a TUNEL assay. And expression up-regulation of p53 and Bax, and down-regulation of Bcl-2 were observed (p<0.05), indicating the apoptosis is related to p53 mediated pathway.
authors.affiliation: College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China. Electronic address: deyonghz@163.com.
authors.forename: De-Yong
authors.initials: DY
authors.lastname: Zhang
authors.affiliation: College of Biology and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310012, China.
authors.forename: Xiu-Ying
authors.initials: XY
authors.lastname: Shen
authors.affiliation: College of Xingzhi, Zhejiang Normal University, Jinhua 321004, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Ruan
authors.affiliation: College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China.
authors.forename: Xiao-Lu
authors.initials: XL
authors.lastname: Xu
authors.affiliation: College of Xingzhi, Zhejiang Normal University, Jinhua 321004, China.
authors.forename: San-Ping
authors.initials: SP
authors.lastname: Yang
authors.affiliation: College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Lu
authors.affiliation: College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China.
authors.forename: Hui-Ying
authors.initials: HY
authors.lastname: Xu
authors.affiliation: College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China.
authors.forename: Fei-Lin
authors.initials: FL
authors.lastname: Hao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D008813:Mice, Inbred ICR; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D012493:Samarium / Q000633:toxicity*; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013739:Testosterone / Q000097:blood; D051028:bcl-2-Associated X Protein / Q000235:genetics
chemical_list: C494827:Bax protein, mouse; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D013739:Testosterone; D012493:Samarium; D007986:Luteinizing Hormone
keywords: Apoptosis; Bcl-2; Mouse testis; Samarium nitrate; Ultra-structure