title: A high-throughput protocol for message RNA quantification using RNA dot-blots.
abstract: This study develops a method to rapidly measure the relative abundance of mRNA in total RNA samples using a dot-blotting technique and biotin-labeled detection probes that recognize the polyadenylate tail on mRNA. We demonstrate the effectiveness of this technique by determining the relative total amounts of mRNA in three tissues of turtles (Trachemys scripta elegans) exposed to normoxic versus anoxic conditions. The data emphasize the usefulness of the method for the simple and rapid analysis of relative total mRNA levels for a variety of comparison purposes.
authors.affiliation: Institute of Biochemistry and Department of Biology, Carleton University, Ottawa, ON K1S 5B6, Canada.
authors.forename: Andrew N
authors.initials: AN
authors.lastname: Rouble
authors.affiliation: Institute of Biochemistry and Department of Biology, Carleton University, Ottawa, ON K1S 5B6, Canada.
authors.forename: Kyle K
authors.initials: KK
authors.lastname: Biggar
authors.affiliation: Institute of Biochemistry and Department of Biology, Carleton University, Ottawa, ON K1S 5B6, Canada. Electronic address: kenneth_storey@carleton.ca.
authors.forename: Kenneth B
authors.initials: KB
authors.lastname: Storey
mesh_terms: D000818:Animals; D015151:Immunoblotting / Q000379:methods*; D018384:Oxidative Stress; D012333:RNA, Messenger / Q000032:analysis / Q000302:isolation & purification; D013997:Time Factors; D014426:Turtles
chemical_list: D012333:RNA, Messenger
keywords: Anoxia tolerance; Dot-blot ribonucleotide hybridization; Stress-responsive mRNA analysis; Turtle