title: Genetic variation of bovine leukemia virus (BLV) after replication in cell culture and experimental animals.
abstract: This article reports the selection of bovine leukemia virus (BLV) variants after continuous passage in cell lines or experimental animals. Two wild BLV strains isolated from 2 naturally infected Holstein dairy cows in Brazil (cow codes: 485 and 141) were used for the experimental infection of 1 sheep and FLK cells, and 1 rabbit and CC81 cells. Viral DNA was isolated several months after infection, and env gene nucleotide and amino acid sequences of the "passaged" variants were compared against the 2 original infecting wild strains. The sequences of the original infecting wild strains were not recovered after their replication in the cell lines or experimental animals. These results indicate that genetic variation occurred after BLV replication in vivo and in vitro, with new variants being selected.
authors.affiliation: Laboratório Nacional Agropecuário, Ministério da Agricultura, Pecuária e Abastecimento, Pedro Leopoldo, MG, Brasil.
authors.forename: M F
authors.initials: MF
authors.lastname: Camargos
authors.affiliation: Laboratório de Retroviroses, Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
authors.forename: D S
authors.initials: DS
authors.lastname: Rajão
authors.affiliation: Laboratório de Retroviroses, Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
authors.forename: R C
authors.initials: RC
authors.lastname: Leite
authors.affiliation: Laboratório de Retroviroses, Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
authors.forename: D
authors.initials: D
authors.lastname: Stancek
authors.affiliation: Laboratório de Retroviroses, Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
authors.forename: M B
authors.initials: MB
authors.lastname: Heinemann
authors.affiliation: Laboratório de Retroviroses, Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil jenner@ufmg.br.
authors.forename: J K P
authors.initials: JK
authors.lastname: Reis
mesh_terms: D000818:Animals; D001483:Base Sequence; D001938:Brazil; D002417:Cattle; D002455:Cell Division; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics*; D015752:Genes, env; D001909:Leukemia Virus, Bovine / Q000235:genetics* / Q000472:pathogenicity; D011817:Rabbits; D012756:Sheep; D014779:Virus Replication / Q000235:genetics*
chemical_list: D004279:DNA, Viral