title: Isolation and synthesis of pulmonarins A and B, acetylcholinesterase inhibitors from the colonial ascidian Synoicum pulmonaria.
abstract: Pulmonarins A and B are two new dibrominated marine acetylcholinesterase inhibitors that were isolated and characterized from the sub-Arctic ascidian Synoicum pulmonaria collected off the Norwegian coast. The structures of natural pulmonarins A and B were tentatively elucidated by spectroscopic methods and later verified by comparison with synthetically prepared material. Both pulmonarins A and B displayed reversible, noncompetitive acetylcholinesterase inhibition comparable to several known natural acetylcholinesterase inhibitiors. Pulmonarin B was the strongest inhibitor, with an inhibition constant (Ki) of 20 μM. In addition to reversible, noncompetitive acetylcholinesterase inhibition, the compounds displayed weak antibacterial activity but no cytotoxicity or other investigated bioactivities.
authors.affiliation: Norwegian College of Fishery Science, University of Tromsø , Breivika N-9037, Tromsø, Norway.
authors.forename: Margey
authors.initials: M
authors.lastname: Tadesse
authors.forename: Johan
authors.initials: J
authors.lastname: Svenson
authors.forename: Kristina
authors.initials: K
authors.lastname: Sepčić
authors.forename: Laurent
authors.initials: L
authors.lastname: Trembleau
authors.forename: Magnus
authors.initials: M
authors.lastname: Engqvist
authors.forename: Jeanette H
authors.initials: JH
authors.lastname: Andersen
authors.forename: Marcel
authors.initials: M
authors.lastname: Jaspars
authors.forename: Klara
authors.initials: K
authors.lastname: Stensvåg
authors.forename: Tor
authors.initials: T
authors.lastname: Haug
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001969:Bromobenzenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D048230:Corynebacterium glutamicum / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D008386:Marine Biology; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D014561:Urochordata / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D001969:Bromobenzenes; D002800:Cholinesterase Inhibitors; C000590253:pulmonarin A; C000590254:pulmonarin B