title: Axial nanoscale localization by normalized total internal reflection fluorescence microscopy.
abstract: We present a simple modification of a standard total internal reflection fluorescence microscope to achieve nanometric axial resolution, typically ≈10  nm. The technique is based on a normalization of total internal reflection images by conventional epi-illumination images. We demonstrate the potential of our method to study the adhesion of phopholipid giant unilamellar vesicles.
authors.forename: Marcelina Cardoso
authors.initials: MC
authors.lastname: Dos Santos
authors.forename: Régis
authors.initials: R
authors.lastname: Déturche
authors.forename: Cyrille
authors.initials: C
authors.lastname: Vézy
authors.forename: Rodolphe
authors.initials: R
authors.lastname: Jaffiol
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence / Q000379:methods*; D053835:Unilamellar Liposomes / Q000378:metabolism
chemical_list: D053835:Unilamellar Liposomes