title: The dynamic detection of NO during the ischemic postconditioning against global cerebral ischemia/reperfusion injury.
abstract: Little is known about role of Nitric Oxide (NO) of ischemic postconditioning in global cerebral ischemia and reperfusion (I/R) injury. In this study, we detected the dynamic change of NO during the ischemic postconditioning against global cerebral I/R in vivo, and compared the effects of six postconditioning conditions with different numbers of cycles and periods for reperfusion/occlusion. The animals underwent postconditioning consisting of 3 or 10 cycles of 15-s reperfusion, followed by 15-s occlusion (post-3-15/15, post-10-15/15), or 3 or 10 cycles of 30-s reperfusion/30-s occlusion (post-3-30/30, post-10-30/30), or 3 or 10 cycles of 60-s reperfusion/15-s occlusion (post-3-60/15, post-10-60/15). The results showed that four groups increased NO concentration, while all groups improved CBF significantly. Different postconditioning groups had different effects on NO and CBF. Post-3-30/30 had the strongest effect on both. Also it reduced infarct size from 33.0% to 24.29%, and downregulated the cell death ratio from 6.71% to 1.04%, showing the strongest protective effect among tested conditions. And we found that post-3-30/30 was an optional method in ischemic postconditioning, which obviously induced a large amount of NO synthesis with a slow speed, increased CBF and reduced the brain injuries. Therefore we concluded that NO is a reliable candidate in mediating ischemic postconditioning neuroprotection.
authors.affiliation: College of Life Information Science and Instrument Engineering, Hangzhou Dianzi University, China. Electronic address: liukezhou2013@gmail.com.
authors.forename: Kezhou
authors.initials: K
authors.lastname: Liu
authors.affiliation: College of Life Information Science and Instrument Engineering, Hangzhou Dianzi University, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yan
authors.affiliation: Biomedical Engineering, Zhejiang University, China.
authors.forename: Xiaoxiang
authors.initials: X
authors.lastname: Zheng
authors.affiliation: College of Life Information Science and Instrument Engineering, Hangzhou Dianzi University, China. Electronic address: yyang@hdu.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply* / Q000378:metabolism*; D002545:Brain Ischemia / Q000378:metabolism*; D057775:Ischemic Postconditioning; D008297:Male; D018696:Neuroprotective Agents / Q000378:metabolism; D009569:Nitric Oxide / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism* / Q000517:prevention & control*
chemical_list: D018696:Neuroprotective Agents; D009569:Nitric Oxide
keywords: Global cerebral ischemia; Ischemic postconditioning; Nitric Oxide