title: Recombinant murine IL-12 promotes a protective Th1/cellular response in Mongolian gerbils infected with Sporothrix schenckii.
abstract: Sporotrichosis is a cutaneous fungal infection caused by Sporothrix schenckii. It is known to be mainly contained by Th1 responses. As IL-12 is crucial for Th1 response, we investigated if treatment with recombinant murine IL-12 (rmIL-12) promoted Th1 immunity and/or clinical improvement in an experimental sporotrichosis gerbil model. Gerbils were inoculated with S. schenckii in the footpad and treated with rmIL-12. Seven days post infection there was a significant increase in macrophage phagocytosis and oxidative burst, and in delayed-type hypersensitivity (DTH) reaction in rmIL-12 treated gerbils, as well as a ∼10-fold increase of serum IFN-gamma and a decrease of IL-4 and IL-10. Moreover, rmIL-12 substantially decreased (∼70%) S. schenckii burden in liver and spleen and improved the clinical outcome preventing footpad ulcer and tail nodules observed in untreated gerbils. Our study demonstrates that rmIL-12 promotes Th1 immune response against S. schenckii favouring its clearance and preventing clinical symptoms.
authors.forename: Aurelio
authors.initials: A
authors.lastname: Flores-García
authors.forename: Jesús Salvador
authors.initials: JS
authors.lastname: Velarde-Félix
authors.forename: Vicente
authors.initials: V
authors.lastname: Garibaldi-Becerra
authors.forename: Héctor
authors.initials: H
authors.lastname: Rangel-Villalobos
authors.forename: Olivia
authors.initials: O
authors.lastname: Torres-Bugarín
authors.forename: Eloy Alfonso
authors.initials: EA
authors.lastname: Zepeda-Carrillo
authors.forename: Salvador
authors.initials: S
authors.lastname: Ruíz-Bernés
authors.forename: Luis Antonio
authors.initials: LA
authors.lastname: Ochoa-Ramírez
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000378:metabolism / Q000627:therapeutic use; D002478:Cells, Cultured; D018920:Coculture Techniques; D004342:Drug Hypersensitivity / Q000276:immunology / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control; D005849:Gerbillinae; D007111:Immunity, Cellular / Q000187:drug effects; D007113:Immunity, Innate / Q000187:drug effects*; D007155:Immunologic Factors / Q000235:genetics / Q000378:metabolism / Q000627:therapeutic use*; D018664:Interleukin-12 / Q000235:genetics / Q000378:metabolism / Q000627:therapeutic use*; D008099:Liver / Q000187:drug effects / Q000276:immunology / Q000382:microbiology; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D008297:Male; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects; D011994:Recombinant Proteins / Q000378:metabolism / Q000627:therapeutic use; D016897:Respiratory Burst / Q000187:drug effects; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000187:drug effects / Q000276:immunology / Q000382:microbiology; D013175:Sporothrix / Q000187:drug effects / Q000276:immunology* / Q000302:isolation & purification; D013174:Sporotrichosis / Q000188:drug therapy* / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D018417:Th1 Cells / Q000187:drug effects* / Q000276:immunology / Q000382:microbiology
chemical_list: D000935:Antifungal Agents; D007155:Immunologic Factors; D011994:Recombinant Proteins; D018664:Interleukin-12
keywords: IL-12,; Meriones unguiculatus,; Sporothrix schenckii,; Sporotrichosis,; Th1 response