title: Improving bgl1 gene expression in Saccharomyces cerevisiae through meiosis in an isogenic triploid.
abstract: Introducing large numbers of target genes into the chromosome of Saccharomyces cerevisiae via δ-sequence-mediated integration is a good strategy for exploring the effects of gene dosage on expression and secretion of heterologous proteins. The expression of exogenous genes might be further improved through meiosis in an isogenic triploid. Here, a stable strain A-8 was screened from 35 sexual spore colonies obtained from an isogenic triploid integratively expressing bgl1 from Aspergillus aculeatus. The corresponding β-glucosidase activity in this strain was increased by ~120 % compared with the parent strain BGL-a. Measurement of doubling time, flow cytometry, and mating experiments further confirmed that A-8 was a spore-forming strain obtained from a triploid parent. Thus, combining δ-integration and meiosis in an isogenic triploid is a promising approach for improving the expression of exogenous proteins in S. cerevisiae.
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China, huajunyang@163.com.
authors.forename: Huajun
authors.initials: H
authors.lastname: Yang
authors.forename: Cheng
authors.initials: C
authors.lastname: Liu
authors.forename: Shaolan
authors.initials: S
authors.lastname: Zou
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Ma
authors.forename: Jiefang
authors.initials: J
authors.lastname: Hong
authors.forename: Minhua
authors.initials: M
authors.lastname: Zhang
mesh_terms: D001230:Aspergillus / Q000201:enzymology / Q000235:genetics; D015870:Gene Expression; D043326:Glucan 1,3-beta-Glucosidase / Q000096:biosynthesis* / Q000235:genetics; D008540:Meiosis; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D057885:Triploidy
chemical_list: D011994:Recombinant Proteins; D043326:Glucan 1,3-beta-Glucosidase