title: Radioadaptive response in human lymphocyte cells.
abstract: The adaptive response (AR) is a phenomenon by which cells exposure to sublethal doses of DNA-damaging agents (non-mutagenic dose of chemical or radiation), known as conditioning treatment (CT), leads to increased resistance to a subsequent exposure to a higher dose of the same or other agents, known as challenge treatment (CR). The adaptive response (AR) induced by radiation in human lymphocytes has been reported in a range of 1-20cGy pre-exposure. In this study, we investigated the adaptive response using 5cGy conditioning dose of gamma rays followed by 2 Gy challenging dose in peripheral human lymphocyte cells. Blood samples were taken from 30 female volunteers and this experiment was carried out by delivering 5 cGy gamma radiation followed by 2 Gy of challenging. Consequently, the number of micronuclei (MN) in binuclear lymphocyte cells was counted as an endpoint. The results showed that the mean frequency of micronuclei in binuclear lymphocytes which have received both conditioning and challenge doses are significantly reduced in comparison to those only exposed to 2 Gy (20.46±2.13, 30.2±3.29) (P< 0.01). The results showed the existence of an in vitro adaptive response in lymphocyte cell exposed to low dose of gamma radiations.
authors.affiliation: Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Najmeh
authors.initials: N
authors.lastname: Assadi
authors.affiliation: Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Zabihi
authors.affiliation: Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Meysam
authors.initials: M
authors.lastname: Khosravifarsani
authors.affiliation: Social Medicine and Health Department, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Soraya
authors.initials: S
authors.lastname: Khafri
authors.affiliation: Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Haleh
authors.initials: H
authors.lastname: Akhavanniaki
authors.affiliation: Department of Nuclear Medicine, Paramedical Faculty, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Mehrangiz
authors.initials: M
authors.lastname: Amiri
authors.affiliation: Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Shabestani-Monfared
keywords: Adaptive Response (AR); challenge treatment (CR); condition treatment (CT); micronuclei assay