title: Function and localization dynamics of bifunctional penicillin-binding proteins in Caulobacter crescentus.
abstract: The peptidoglycan cell wall of bacteria is a complex macromolecule composed of glycan strands that are cross-linked by short peptide bridges. Its biosynthesis involves a conserved group of enzymes, the bifunctional penicillin-binding proteins (bPBPs), which contain both a transglycosylase and a transpeptidase domain, thus being able to elongate the glycan strands and, at the same time, generate the peptide cross-links. The stalked model bacterium Caulobacter crescentus possesses five bPBP paralogs, named Pbp1A, PbpC, PbpX, PbpY, and PbpZ, whose function is still incompletely understood. In this study, we show that any of these proteins except for PbpZ is sufficient for growth and normal morphogenesis when expressed at native or elevated levels, whereas inactivation of all five paralogs is lethal. Growth analyses indicate a central role of PbpX in the resistance of C. crescentus against the noncanonical amino acid d-alanine. Moreover, we show that PbpX and PbpY localize to the cell division site. Their recruitment to the divisome is dependent on the essential cell division protein FtsN and likely involves interactions with FtsL and the putative peptidoglycan hydrolase DipM. The same interaction pattern is observed for Pbp1A and PbpC, although these proteins do not accumulate at midcell. Our findings demonstrate that the bPBPs of C. crescentus are, to a large extent, redundant and have retained the ability to interact with the peptidoglycan biosynthetic machineries responsible for cell elongation, cytokinesis, and stalk growth. Nevertheless, they may preferentially act in specific peptidoglycan biosynthetic complexes, thereby facilitating the independent regulation of distinct growth processes.
authors.affiliation: Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Strobel
authors.forename: Andrea
authors.initials: A
authors.lastname: Möll
authors.forename: Daniela
authors.initials: D
authors.lastname: Kiekebusch
authors.forename: Kathrin E
authors.initials: KE
authors.lastname: Klein
authors.forename: Martin
authors.initials: M
authors.lastname: Thanbichler
mesh_terms: D000595:Amino Acid Sequence; D016935:Caulobacter crescentus / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development / Q000502:physiology*; D002473:Cell Wall / Q000378:metabolism; D055786:Gene Knockout Techniques; D050296:Microbial Viability; D008853:Microscopy; D008969:Molecular Sequence Data; D046915:Penicillin-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D010457:Peptidoglycan / Q000096:biosynthesis*; D011485:Protein Binding; D025941:Protein Interaction Mapping
chemical_list: D046915:Penicillin-Binding Proteins; D010457:Peptidoglycan