title: A simple method for screening of plant NBS-LRR genes that confer a hypersensitive response to plant viruses and its application for screening candidate pepper genes against Pepper mottle virus.
abstract: Plant NBS-LRR genes are abundant and have been increasingly cloned from plant genomes. In this study, a method based on agroinfiltration and virus inoculation was developed for the simple and inexpensive screening of candidate R genes that confer a hypersensitive response to plant viruses. The well-characterized resistance genes Rx and N, which confer resistance to Potato virus X (PVX) and tobamovirus, respectively, were used to optimize a transient expression assay for detection of hypersensitive response in Nicotiana benthamiana. Infectious sap of PVX and Tobacco mosaic virus were used to induce hypersensitive response in Rx- and N-infiltrated leaves, respectively. The transient expression of the N gene induced local hypersensitive response upon infection of another tobamovirus, Pepper mild mottle virus, through both sap and transcript inoculation. When this method was used to screen 99 candidate R genes from pepper, an R gene that confers hypersensitive response to the potyvirus Pepper mottle virus was identified. The method will be useful for the identification of plant R genes that confer resistance to viruses.
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea; Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Phu-Tri
authors.initials: PT
authors.lastname: Tran
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea; Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Hoseong
authors.initials: H
authors.lastname: Choi
authors.affiliation: Department of Plant Science, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea; Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Saet-Byul
authors.initials: SB
authors.lastname: Kim
authors.affiliation: Department of Plant Science, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea; Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Hyun-Ah
authors.initials: HA
authors.lastname: Lee
authors.affiliation: Department of Plant Science, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea; Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea; Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul 151-921, Republic of Korea.
authors.forename: Doil
authors.initials: D
authors.lastname: Choi
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea; Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea; Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul 151-921, Republic of Korea. Electronic address: kookkim@snu.ac.kr.
authors.forename: Kook-Hyung
authors.initials: KH
authors.lastname: Kim
mesh_terms: D002212:Capsicum / Q000235:genetics*; D017343:Genes, Plant; D005820:Genetic Testing / Q000379:methods*; D017800:Potyvirus / Q000276:immunology*; D014026:Nicotiana / Q000235:genetics / Q000276:immunology / Q000502:physiology* / Q000821:virology*
keywords: Hypersensitive response; NBS-LRR genes; Pepper; Pepper mottle virus; Screening method; Virus resistance