title: PHA production by mixed cultures: a way to valorize wastes from pulp industry.
abstract: In this work, hardwood spent sulfite liquor (HSSL), a complex feedstock originating from the pulp industry, was tested for the first time as a substrate for polyhydroxyalkanoate (PHA) production by a mixed microbial culture (MMC) under aerobic dynamic feeding (ADF) conditions. A sequencing batch reactor (SBR) fed with HSSL was operated for 67days and the selected MMC reached a maximum PHA content of 67.6%. The MMC demonstrated a differential utilization of the carbon sources present in HSSL. Acetic acid was fully consumed, while xylose and lignosulphonates were partially consumed (30% and 14%, respectively). The selected culture was characterized by Fluorescence in Situ Hybridization (FISH). Bacteria belonging to the three main classes were identified: Alpha- (72.7±4.0%), Beta- (11.1±0.37%) and Gammaproteobacteria (10.3±0.3%). Within Alphaproteobacteria, a small amount of Paracoccus (4.2±0.51%) and Defluvicoccus related to Tetrad Forming Organisms (9.0±0.28%) were detected.
authors.affiliation: CICECO, Departamento de Química, Universidade de Aveiro, Campus Universitário de Santiago, P-3810-193 Aveiro, Portugal.
authors.forename: Diogo
authors.initials: D
authors.lastname: Queirós
authors.affiliation: Water Research Institute - CNR, Via Salaria Km 29,300, 00015 Monterotondo, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Rossetti
authors.affiliation: CICECO, Departamento de Química, Universidade de Aveiro, Campus Universitário de Santiago, P-3810-193 Aveiro, Portugal. Electronic address: luisa.serafim@ua.pt.
authors.forename: Luísa S
authors.initials: LS
authors.lastname: Serafim
mesh_terms: D019342:Acetic Acid / Q000032:analysis; D064751:Ammonium Compounds / Q000032:analysis; D001419:Bacteria / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D057919:Biological Oxygen Demand Analysis; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D007220:Industrial Waste; D007700:Kinetics; D010100:Oxygen / Q000032:analysis; D010209:Paper; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*
chemical_list: D064751:Ammonium Compounds; D007220:Industrial Waste; D054813:Polyhydroxyalkanoates; D019342:Acetic Acid; D010100:Oxygen
keywords: Aerobic dynamic feeding; FISH; HSSL; Mixed microbial cultures; Polyhydroxyalkanoates