title: OPTiSPIM: integrating optical projection tomography in light sheet microscopy extends specimen characterization to nonfluorescent contrasts.
abstract: Mesoscopic 3D imaging has become a widely used optical imaging technique to visualize intact biological specimens. Selective plane illumination microscopy (SPIM) visualizes samples up to a centimeter in size with micrometer resolution by 3D data stitching but is limited to fluorescent contrast. Optical projection tomography (OPT) works with fluorescent and nonfluorescent contrasts, but its resolution is limited in large samples. We present a hybrid setup (OPTiSPIM) combining the advantages of each technique. The combination of fluorescent and nonfluorescent high-resolution 3D data into integrated datasets enables a more extensive representation of mesoscopic biological samples. The modular concept of the OPTiSPIM facilitates incorporation of the transmission OPT modality into already established light sheet based imaging setups.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Mayer
authors.forename: Alexandre
authors.initials: A
authors.lastname: Robert-Moreno
authors.forename: Renzo
authors.initials: R
authors.lastname: Danuser
authors.forename: Jens V
authors.initials: JV
authors.lastname: Stein
authors.forename: James
authors.initials: J
authors.lastname: Sharpe
authors.forename: Jim
authors.initials: J
authors.lastname: Swoger
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000166:cytology; D008027:Light; D008198:Lymph Nodes / Q000166:cytology; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D041622:Tomography, Optical / Q000379:methods*