title: A proteomic approach to porcine saliva.
abstract: This paper reviews recent progress in salivary animal proteomics, with special reference to the porcine proteome. Until fairly recently, most studies on saliva as a diagnostic fluid have focused on humans, primates and rodents, and the development of salivary analysis in monitoring health in farm animals including pigs has received only limited consideration. The porcine salivary proteome has been characterised by 2D-electrophoresis followed by mass spectrometry. Major and minor proteins have been identified. The use of saliva as a non-invasive biological fluid in monitoring health and disease in pigs will be reviewed, together with the potential use of proteomics for the development of biomarkers. In this review, methods of collection and the composition of porcine saliva will be considered, together with saliva handling and analysis. The overall findings indicate that there is considerable potential for the development of salivary analysis as a non-invasive diagnostic fluid in the pig, and that it offers advantages over other body fluids in this animal.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Gutiérrez
authors.forename: José J
authors.initials: JJ
authors.lastname: Cerón
authors.forename: María
authors.initials: M
authors.lastname: Fuentes-Rubio
authors.forename: Fernando
authors.initials: F
authors.lastname: Tecles
authors.affiliation: Department of Animal Medicine and Surgery, University of Murcia, 30100 Espinardo, Murcia, Spain. ftecles@um.es.
authors.forename: Josie A
authors.initials: JA
authors.lastname: Beeley
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000914:Antibodies, Viral / Q000032:analysis*; D001424:Bacterial Infections / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D015415:Biomarkers / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D040901:Proteomics; D012463:Saliva / Q000737:chemistry*; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D013048:Specimen Handling / Q000379:methods; D013312:Stress, Physiological; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology / Q000821:virology; D014777:Virus Diseases / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary* / Q000821:virology
chemical_list: D000907:Antibodies, Bacterial; D000914:Antibodies, Viral; D015415:Biomarkers; D012471:Salivary Proteins and Peptides