title: Application of bacteriophages for detection of foodborne pathogens.
abstract: Bacterial contamination of food products presents a challenge for the food industry and poses a high risk for the consumer. Despite increasing awareness and improved hygiene measures, foodborne pathogens remain a threat for public health, and novel methods for detection of these organisms are needed. Bacteriophages represent ideal tools for diagnostic assays because of their high target cell specificity, inherent signal-amplifying properties, easy and inexpensive production, and robustness. Every stage of the phage lytic multiplication cycle, from the initial recognition of the host cell to the final lysis event, may be harnessed in several ways for the purpose of bacterial detection. Besides intact phage particles, phage-derived affinity molecules such as cell wall binding domains and receptor binding proteins can serve for this purpose. This review provides an overview of existing phage-based technologies for detection of foodborne pathogens, and highlights the most recent developments in this field, with particular emphasis on phage-based biosensors.
authors.affiliation: Institute of Food, Nutrition and Health; ETH Zurich; Zurich, Switzerland.
authors.forename: Mathias
authors.initials: M
authors.lastname: Schmelcher
authors.affiliation: Institute of Food, Nutrition and Health; ETH Zurich; Zurich, Switzerland.
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Loessner
keywords: bacterial detection; biosensor; cell wall binding domain; diagnostics; foodborne pathogens; phage amplification; receptor binding protein; reporter phage