title: Protective effects of sulphonated formononetin in a rat model of cerebral ischemia and reperfusion injury.
abstract: Sodium formononetin-3'-sulphonate is a derivative of the plant isoflavone formononetin. The present study aimed to investigate the neuroprotective and angiogenesis effects of sodium formononetin-3'-sulphonate in vivo and in vitro. Treatment with sodium formononetin-3'-sulphonate (3, 7.5, 15, and 30 mg/kg, intravenous injection) could protect the brain from ischemia and reperfusion injury by improving neurological function, suppressing cell apoptosis, and increasing expression levels of vascular endothelial growth factor and platelet endothelial cell adhesion molecule 1 by middle cerebral artery occlusion. Treatment with sodium formononetin-3'-sulphonate (10 and 20 µg/mL) significantly increased cell migration, tube formation, and vascular endothelial growth factor and platelet endothelial cell adhesion molecule levels in human umbilical vein endothelial cells. Our results suggest that sodium formononetin-3'-sulphonate provides significant neuroprotective effects against cerebral ischemia and reperfusion injury in rats, and improves cerebrovascular angiogenesis in human umbilical vein endothelial cells. The protective mechanisms of sodium formononetin-3'-sulphonate may be attributed to the suppression of cell apoptosis and improved cerebrovascular angiogenesis by promoting vascular endothelial growth factor and platelet endothelial cell adhesion molecule expression.
authors.affiliation: Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, PR China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, PR China.
authors.forename: Libo
authors.initials: L
authors.lastname: Zou
authors.affiliation: School of Pharmacy, Yantai University, Yantai, PR China.
authors.forename: Jingwei
authors.initials: J
authors.lastname: Tian
authors.affiliation: State Key Laboratory of Long-acting and Targeting Drug Delivery Technologies (Luye Pharma Group Ltd.), Yantai, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Long-acting and Targeting Drug Delivery Technologies (Luye Pharma Group Ltd.), Yantai, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: He
authors.affiliation: State Key Laboratory of Long-acting and Targeting Drug Delivery Technologies (Luye Pharma Group Ltd.), Yantai, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Hou
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D027883:Astragalus Plant / Q000737:chemistry*; D001921:Brain / Q000098:blood supply / Q000187:drug effects*; D002545:Brain Ischemia / Q000188:drug therapy*; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D020244:Infarction, Middle Cerebral Artery; D007275:Injections, Intravenous; D007529:Isoflavones / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*; D042461:Vascular Endothelial Growth Factor A / Q000097:blood
chemical_list: D043925:Angiogenesis Inducing Agents; D007529:Isoflavones; D018696:Neuroprotective Agents; D010936:Plant Extracts; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D042461:Vascular Endothelial Growth Factor A; C548268:formononetin-3-sulfonate