title: Conversion of salvianolic acid B into salvianolic acid A in tissues of Radix Salviae Miltiorrhizae using high temperature, high pressure and high humidity.
abstract: Salvianolic acid A (Sal A), an important constituent of Radix Salviae Miltiorrhizae (RSM), is effective for the treatment of myocardial infarction (MI) and coronary heart disease due to its potential in the improvement of acute myocardial ischemia. However, its content is very low in RSM. So it is obvious to find a rich source of Sal A or to improve its content by conversion of other related components into Sal A modifying reaction conditions. In this research we focused on the conversion of Sal B into Sal A in aqueous solutions of RSM by using different reaction conditions including pH, temperature, pressure and humidity. During the reactions, the contents of Sal A, Sal B and danshensu in the RSM were analyzed by high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LCMS). The results indicated that the conversion of Sal B into Sal A in RSM tissues under the conditions of a high temperature, high pressure and high humidity was efficient and thereby, was readily utilized to prepare rich Sal A materials in practice.
authors.affiliation: Department of National Products Chemistry, Key Lab of Chemical Biology (MOE), School of Pharmaceutical Sciences, Shandong University, Jinan 250012, PR China.
authors.forename: Hongrui
authors.initials: H
authors.lastname: Xia
authors.affiliation: Department of National Products Chemistry, Key Lab of Chemical Biology (MOE), School of Pharmaceutical Sciences, Shandong University, Jinan 250012, PR China. Electronic address: sunlr@sdu.edu.cn.
authors.forename: Longru
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of National Products Chemistry, Key Lab of Chemical Biology (MOE), School of Pharmaceutical Sciences, Shandong University, Jinan 250012, PR China.
authors.forename: Hongxiang
authors.initials: H
authors.lastname: Lou
authors.affiliation: Medicine Research Group, School of Health, Sport and Bioscience, University of East London, Water Lane, London E15 4LZ, UK.
authors.forename: M Mukhlesur
authors.initials: MM
authors.lastname: Rahman
mesh_terms: D001572:Benzofurans / Q000737:chemistry*; D002109:Caffeic Acids / Q000138:chemical synthesis* / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D006358:Hot Temperature; D006813:Humidity; D007773:Lactates / Q000032:analysis / Q000138:chemical synthesis* / Q000627:therapeutic use; D013058:Mass Spectrometry; D017202:Myocardial Ischemia / Q000188:drug therapy; D008517:Phytotherapy; D018517:Plant Roots; D011312:Pressure; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D053630:Depsides
chemical_list: D001572:Benzofurans; D002109:Caffeic Acids; D004365:Drugs, Chinese Herbal; D007773:Lactates; C028972:dan-shen root extract; C035055:3,4-dihydroxyphenyllactic acid; C066201:salvianolic acid A; C076944:salvianolic acid B; D053630:Depsides
keywords: Conversion; Danshensu; Radix Salviae Miltiorrhizae (RSM); Salvianolic acid A; Salvianolic acid B