title: Engineered riboswitch as a gene-regulatory platform for reducing antibiotic resistance.
abstract: Antibiotic resistance (AR), the ability of a microorganism to withstand the effects of antibiotics, is a growing and increasingly serious global public health problem. Enzymatic activation of antibiotics though the production of β-lactamase is one of the main mechanisms causing AR. Synthetic riboswitch containing aptazyme is constructed in E. coli to regulate the expression of β-lactamase through small molecule-aptamer interactions, which sharply reduces the antibiotic resistance of the engineered bacteria.
authors.affiliation: Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, People's Republic of China.
authors.forename: Libing
authors.initials: L
authors.lastname: Liu
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D052157:Aptamers, Nucleotide / Q000378:metabolism; D001483:Base Sequence; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D015964:Gene Expression Regulation, Bacterial; D005818:Genetic Engineering / Q000379:methods*; D010957:Plasmids / Q000235:genetics; D058928:Riboswitch; D013806:Theophylline / Q000378:metabolism; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D052157:Aptamers, Nucleotide; D058928:Riboswitch; D013806:Theophylline; D001618:beta-Lactamases