title: Short communication: prevalence and antibiotic resistance of Staphylococcus aureus isolated from bovine clinical mastitis.
abstract: The aims of this study were to determine the prevalence and antibiotic resistance of Staphylococcus aureus isolated from bovine clinical mastitis in Varamin, Tehran Province, Iran. All of the isolated Staph. aureus were identified by morphology and culture and confirmed using the API Staph identification system (bioMérieux, Marcy-l'Étoile, France). Antibiotic resistance genes were detected by PCR with oligonucleotide primers specific for each gene. Staphylococcus aureus was recovered from 43 of 207 (20.1%) bovine clinical milk samples. Using disk diffusion, methicillin-resistant Staph. aureus was detected in 5 of 43 (11.6%) samples. The pathogen showed high resistance against penicillin G (86%) and tetracycline (76.7%). The blaZ (penicillin) (86%), tetM (tetracycline), and ermC (erythromycin) genes (39.5% each) were the most prevalent antibiotic resistance genes. The findings of this study are useful for designing specific control programs for bovine clinical mastitis caused by Staph. aureus in this region of Iran.
authors.affiliation: Biohealth Science Program, Institute of Biological Science, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.
authors.forename: Hossein
authors.initials: H
authors.lastname: Jamali
authors.affiliation: Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Islamic Azad University-Karaj Branch, Karaj 31485-313, Iran.
authors.forename: Behrad
authors.initials: B
authors.lastname: Radmehr
authors.affiliation: Biohealth Science Program, Institute of Biological Science, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia. Electronic address: Salmah_r@um.edu.my.
authors.forename: Salmah
authors.initials: S
authors.lastname: Ismail
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002417:Cattle; D024901:Drug Resistance, Multiple, Bacterial; D005260:Female; D007492:Iran / Q000453:epidemiology; D008414:Mastitis, Bovine / Q000453:epidemiology* / Q000382:microbiology; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D015995:Prevalence; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
chemical_list: D000890:Anti-Infective Agents; D001426:Bacterial Proteins
keywords: Staphylococcus aureus; antibiotic susceptibility; clinical mastitis; resistance gene