title: Cytotoxic oleanane-type triterpenoid saponins from the Rhizomes of Anemone rivularis var. flore-minore.
abstract: Phytochemical investigation of the n-BuOH extract of the rhizomes of Anemone rivularis var. flore-minore led to the isolation of five new oleanane-type triterpenoid saponins 1-5, together with five known saponins 6-10. Their structures were determined by the extensive use of 1D and 2D NMR experiments, along with ESIMS analyses and acid hydrolysis. The aglycone of 4 and 5 was determined as 21α-hydroxyoleanolic acid, which was reported in this genus for the first time. The cytotoxicity of these compounds was evaluated against four human cancer cell line, including HL-60 (promyelocytic leukemia), HepG2 (hepatocellular carcinoma), A549 (lung carcinoma) and HeLa (cervical carcinoma). The monodesmosidic saponins 6-8 exhibited cytotoxic activity toward all tested cancer cell lines, with IC50 values in the 7.25-22.38 μM range.
authors.affiliation: Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. steveplum@sina.com.
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Nuclear Magnetic Resonance Center, Xi'an Modern Chemistry Research Institute, Xi'an 710065, China. wmc204@163.com.
authors.forename: Minchang
authors.initials: M
authors.lastname: Wang
authors.affiliation: Nuclear Magnetic Resonance Center, Xi'an Modern Chemistry Research Institute, Xi'an 710065, China. naturemin2001@163.com.
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.affiliation: Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China. yiwang821203@126.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. tanghaifeng71@163.com.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Tang
authors.affiliation: Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. xiaolis@fmmu.edu.cn.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Sun
mesh_terms: D031883:Anemone / Q000737:chemistry; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000737:chemistry; D004354:Drug Screening Assays, Antitumor; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D009828:Oleanolic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D027343:Rhizome / Q000737:chemistry; D012503:Saponins / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D014315:Triterpenes / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes; C413246:oleanane; D009828:Oleanolic Acid