title: Expression of human transferrin can be regulated effectively by rabbit transferrin regulatory elements in transgenic mice.
abstract: Human transferrin (hTF) belongs to the iron-binding glycoprotein family. It plays an important role in iron transport throughout the body. Transgenic mice are a good model to study how to produce functional hTF on a large-scale. We have improved the expression of hTF and investigated its regulatory mechanism in transgenic mice. Three expression constructs were prepared in which hTF expression was controlled by different regulatory cassettes of rabbit transferrin (rTF). hTF was secreted into serum of transgenic mice when its expression was controlled by the rTF promoter and enhancer, whereas the rTF enhancer in tandem with the rTF promoter repressed hTF secretion into milk. A significant inverse relationship between methylation of the rTF promoter and hTF expression was observed in liver, heart, mammary gland, and muscle of transgenic mice. The highest concentration of hTF was 700 μg/ml in milk.
authors.affiliation: Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai Jiaotong University, 24/1400 West Beijing Road, Shanghai, 200040, People's Republic of China.
authors.forename: Jingbin
authors.initials: J
authors.lastname: Yan
authors.forename: Xiuli
authors.initials: X
authors.lastname: Gong
authors.forename: Shubiao
authors.initials: S
authors.lastname: Pan
authors.forename: Xinbing
authors.initials: X
authors.lastname: Guo
authors.forename: Zhaorui
authors.initials: Z
authors.lastname: Ren
authors.forename: Yitao
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D011817:Rabbits; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D050436:Regulatory Elements, Transcriptional; D014168:Transferrin / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D014168:Transferrin