title: scFv antibodies against infectious bursal disease virus isolated from a combinatorial antibody library by flow cytometry.
abstract: Infectious bursal disease is an economically important disease that affects chickens worldwide. Here, a recombinant single chain variable fragment (scFv) antibody library derived from chickens immunized with VP2 protein of infectious bursal disease virus (IBDV) was constructed. The library was subjected to three rounds of screening by flow cytometry against VP2 protein through a bacteria display technology, resulting in the enrichment of scFv. Three scFv clones with different fluorescence intensity were obtained by random colony pick up. The isolated scFv antibodies were expressed and purified. Relative affinity assay showed the three clones had different sensitivity to VP2, in accordance with fluorescence activity cell sorting analysis. The potential use of the selected IBDV-specific scFv antibodies was demonstrated by the successful application of the isolated antibodies in western blotting assay and ELISA.
authors.affiliation: Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Sciences, Harbin, 150070, People's Republic of China, lmxu0917@163.com.
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Xu
authors.forename: Tian-He
authors.initials: TH
authors.lastname: Li
authors.forename: Bing
authors.initials: B
authors.lastname: Zhou
authors.forename: Mo
authors.initials: M
authors.lastname: Guo
authors.forename: Miao
authors.initials: M
authors.lastname: Liu
authors.forename: Jing-Zhuang
authors.initials: JZ
authors.lastname: Zhao
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Cao
authors.forename: De-Shan
authors.initials: DS
authors.lastname: Li
mesh_terms: D000915:Antibody Affinity / Q000276:immunology; D020650:Combinatorial Chemistry Techniques; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D007243:Infectious bursal disease virus / Q000276:immunology* / Q000378:metabolism; D057127:Single-Chain Antibodies / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D057127:Single-Chain Antibodies