title: Expression of EhRAD54, EhRAD51, and EhBLM proteins during DNA repair by homologous recombination in Entamoeba histolytica.
abstract: Entamoeba histolytica, the protozoan responsible for human amoebiasis, exhibits a great genome plasticity that is probably related to homologous recombination events. It contains the RAD52 epistasis group genes, including Ehrad51 and Ehrad54, and the Ehblm gene, which are key homologous recombination factors in other organisms. Ehrad51 and Ehrad54 genes are differentially transcribed in trophozoites when DNA double-strand breaks are induced by ultraviolet-C irradiation. Moreover, the EhRAD51 recombinase is overexpressed at 30 min in the nucleus. Here, we extend our analysis of the homologous recombination mechanism in E. histolytica by studying EhRAD51, EhRAD54, and EhBLM expression in response to DNA damage. Bioinformatic analyses show that EhRAD54 has the molecular features of homologous proteins, indicating that it may have similar functions. Western blot assays evidence the differential expression of EhRAD51, EhRAD54, and EhBLM at different times after DNA damage, suggesting their potential roles in the different steps of homologous recombination in this protozoan.
authors.affiliation: Programa Institucional de Biomedicina Molecular, Escuela Nacional de Medicina y Homeopatía del IPN, Guillermo Massieu Helguera No. 239, Fracc. La Escalera, Ticoman, México D.F., C.P. 07320, Mexico.
authors.forename: Ma del Socorro
authors.initials: Mdel S
authors.lastname: Charcas-Lopez
authors.affiliation: Programa Institucional de Biomedicina Molecular, Escuela Nacional de Medicina y Homeopatía del IPN, Guillermo Massieu Helguera No. 239, Fracc. La Escalera, Ticoman, México D.F., C.P. 07320, Mexico.
authors.forename: Lorena
authors.initials: L
authors.lastname: Garcia-Morales
authors.affiliation: Programa Institucional de Biomedicina Molecular, Escuela Nacional de Medicina y Homeopatía del IPN, Guillermo Massieu Helguera No. 239, Fracc. La Escalera, Ticoman, México D.F., C.P. 07320, Mexico.
authors.forename: Marisol
authors.initials: M
authors.lastname: Pezet-Valdez
authors.affiliation: Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México, San Lorenzo No. 290, Col. del Valle, México D.F., C.P. 03110, Mexico.
authors.forename: Cesar
authors.initials: C
authors.lastname: Lopez-Camarillo
authors.affiliation: Programa Institucional de Biomedicina Molecular, Escuela Nacional de Medicina y Homeopatía del IPN, Guillermo Massieu Helguera No. 239, Fracc. La Escalera, Ticoman, México D.F., C.P. 07320, Mexico - Programa de Doctorado en Biotecnología, Escuela Nacional de Medicina y Homeopatía del IPN, Guillermo Massieu Helguera No. 239, Fracc. La Escalera, Ticoman, México D.F., C.P. 07320, Mexico.
authors.forename: Absalom
authors.initials: A
authors.lastname: Zamorano-Carrillo
authors.affiliation: Programa Institucional de Biomedicina Molecular, Escuela Nacional de Medicina y Homeopatía del IPN, Guillermo Massieu Helguera No. 239, Fracc. La Escalera, Ticoman, México D.F., C.P. 07320, Mexico - Programa de Doctorado en Biotecnología, Escuela Nacional de Medicina y Homeopatía del IPN, Guillermo Massieu Helguera No. 239, Fracc. La Escalera, Ticoman, México D.F., C.P. 07320, Mexico.
authors.forename: Laurence A
authors.initials: LA
authors.lastname: Marchat
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry; D016384:Consensus Sequence; D003593:Cytoplasm / Q000737:chemistry; D053903:DNA Breaks, Double-Stranded; D004265:DNA Helicases / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D004260:DNA Repair / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics / Q000528:radiation effects; D004748:Entamoeba histolytica / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D017125:Genes, Protozoan; D059765:Homologous Recombination / Q000235:genetics; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D051135:Rad51 Recombinase / Q000235:genetics / Q000502:physiology; D053484:RecQ Helicases / Q000235:genetics / Q000502:physiology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013997:Time Factors; D014466:Ultraviolet Rays
chemical_list: D016054:DNA, Protozoan; D015800:Protozoan Proteins; D051135:Rad51 Recombinase; C097151:Bloom syndrome protein; D004265:DNA Helicases; D053484:RecQ Helicases