title: Lipid and carotenoid production by Rhodotorula glutinis under irradiation/high-temperature and dark/low-temperature cultivation.
abstract: The capacity of lipid and carotenoid production by Rhodotorula glutinis was investigated under different irradiation conditions, temperatures and C/N ratios. The results showed that dark/low-temperature could enhance lipid content, while irradiation/high-temperature increased the yields of biomass and carotenoid. The optimum C/N ratio for production was between 80 and 100. A two-stage cultivation strategy was used for lipid and carotenoid production in a 5L fermenter. In the first stage, the maximum biomass reached 28.1g/L under irradiation/high-temperature. Then, the cultivation condition was changed to dark/low-temperature, and C/N ratio was adjusted to 90. After the second stage, the biomass, lipid content and carotenoid reached 86.2g/L, 26.7% and 4.2mg/L, respectively. More significantly, the yields of biomass and lipid were 43.1% and 11.5%, respectively. Lipids contained 79.7% 18C and 16.8% 16C fatty acids by GC analysis. HPLC quantified the main carotenoids were β-carotene (68.4%), torularhodin (21.5%) and torulene (10.1%).
authors.affiliation: National Energy R&D Center for Biorefinery, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: National Energy R&D Center for Biorefinery, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China. Electronic address: zhangxu@mail.buct.edu.cn.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: National Energy R&D Center for Biorefinery, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China. Electronic address: Twtan@mail.buct.edu.cn.
authors.forename: Tianwei
authors.initials: T
authors.lastname: Tan
mesh_terms: D018533:Biomass; D002244:Carbon / Q000032:analysis; D002338:Carotenoids / Q000096:biosynthesis*; D003080:Cold Temperature; D003624:Darkness; D006358:Hot Temperature; D008027:Light; D008055:Lipids / Q000096:biosynthesis*; D009584:Nitrogen / Q000032:analysis; D012248:Rhodotorula / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects*
chemical_list: D008055:Lipids; D002338:Carotenoids; D002244:Carbon; D009584:Nitrogen
keywords: Carotenoid; Irradiation; Lipid; Rhodotorula glutinis; Two-stage cultivation