title: Characterization of a bioactive fiber scaffold with entrapped HUVECs in coaxial electrospun core-shell fiber.
abstract: Human umbilical vein endothelial cells (HUVECs) were successfully entrapped in polyethylene oxide (PEO) core /polycaprolactone (PCL) shell electrospun fibers thus creating a "bioactive fiber." The viability and release of biomolecules from the entrapped cells in the bioactive fibers were characterized. A key modification to the core solution was the inclusion of 50% fetal bovine serum (FBS), which improved cell viability substantially. The fluorescein diacetate (FDA) staining revealed that the entrapped cells were intact and viable immediately after the electrospinning process. A long-term cell viability assay using AlamarBlue® showed that cells were viable for over two weeks. Secreted Interleukin-8 (IL-8) was monitored as a candidate released protein, which can also act as an indicator of HUVEC stress. These results demonstrated that HUVECs could be entrapped within the electrospun scaffold with the potential of controllable cell deposition and the creation of a bioactive fibrous scaffold with extended functionality.
authors.affiliation: School of Materials and Science Engineering; Division of Materials Technology; Nanyang Technological University; Singapore.
authors.forename: Hui Ying
authors.initials: HY
authors.lastname: Ang
authors.affiliation: School of Materials and Science Engineering; Division of Materials Technology; Nanyang Technological University; Singapore.
authors.forename: Scott Alexander
authors.initials: SA
authors.lastname: Irvine
authors.affiliation: Faculty of Mechanical Engineering; Technion - Israel Institute of Technology; Haifa, Israel.
authors.forename: Ron
authors.initials: R
authors.lastname: Avrahami
authors.affiliation: School of Materials and Science Engineering; Division of Materials Technology; Nanyang Technological University; Singapore.
authors.forename: Udi
authors.initials: U
authors.lastname: Sarig
authors.affiliation: Faculty of Biotechnology and Food Engineering; Technion - Israel Institute of Technology; Haifa, Israel.
authors.forename: Tomer
authors.initials: T
authors.lastname: Bronshtein
authors.affiliation: Faculty of Mechanical Engineering; Technion - Israel Institute of Technology; Haifa, Israel.
authors.forename: Eyal
authors.initials: E
authors.lastname: Zussman
authors.affiliation: School of Materials and Science Engineering; Division of Materials Technology; Nanyang Technological University; Singapore.
authors.forename: Freddy Yin Chiang
authors.initials: FY
authors.lastname: Boey
authors.affiliation: Faculty of Biotechnology and Food Engineering; Technion - Israel Institute of Technology; Haifa, Israel.
authors.forename: Marcelle
authors.initials: M
authors.lastname: Machluf
authors.affiliation: School of Materials and Science Engineering; Division of Materials Technology; Nanyang Technological University; Singapore.
authors.forename: Subbu S
authors.initials: SS
authors.lastname: Venkatraman
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D018914:Cells, Immobilized; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D016062:Porosity; D012996:Solutions / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D016209:Interleukin-8; D011091:Polyesters; D012996:Solutions; C016240:polycaprolactone; D011092:Polyethylene Glycols
keywords: Bioactive fiber; Cell encapsulation; Cell entrapment; Core shell fiber; Electrospinning; HUVECs