title: Expression characteristics of CDC20 in gastric cancer and its correlation with poor prognosis.
abstract: The cell division cycle 20 homolog (CDC20) expression is increased in diverse human cancers and plays a vital role in tumorigenesis and progression. However, the clinical significance of CDC20 expression in gastric cancer (GC) remains largely unknown. The aim of this study was to investigate the clinicopathologic features and prognostic significance of CDC20 in GC. The CDC20 mRNA expression was measured by quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR). Immunohistochemistry (IHC) was used to detect the expression of CDC20 protein in 131 clinicopathologically characterized GC cases. The relationship between CDC20 expression and clinicopathological features was analyzed by appropriate statistics. Kaplan-Meier analysis and Cox proportional hazards regression models were used to investigate the correlation between CDC20 expression and prognosis of GC patients. The relative mRNA expression of CDC20 were significantly higher in GC tumor tissues than in the corresponding noncancerous tissues (P<0.001). Simultaneously, CDC20 protein expression was positively correlated with tumor size (P=0.02), histological grade (P=0.037), lymph node involvement (P=0.009), and TNM stage (P=0.015). Furthermore, Kaplan-Meier analysis indicated that patients with high CDC20 expression had poor overall survival (P<0.001). Multivariate analysis showed that high CDC20 expression was an independent predictor of overall survival. In conclusion, our data indicated that CDC20 upregulation was associated with aggressive progression and poor prognosis in GC. CDC20 was identified for the first time as an independent marker for predicting the clinical outcome of GC patients.
authors.affiliation: Department of General Surgery, Wuxi People's Hospital Affiliated by Nanjing Medical University No. 299, Qingyang Road, Wuxi 214023, China.
authors.forename: Zhong-Yang
authors.initials: ZY
authors.lastname: Ding
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Soochow University No. 1055, Sanxiang Road, Suzhou, 215004, China.
authors.forename: Hao-Rong
authors.initials: HR
authors.lastname: Wu
authors.affiliation: Department of General Surgery, Wuxi People's Hospital Affiliated by Nanjing Medical University No. 299, Qingyang Road, Wuxi 214023, China.
authors.forename: Jia-Ming
authors.initials: JM
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Wuxi People's Hospital Affiliated by Nanjing Medical University No. 299, Qingyang Road, Wuxi 214023, China.
authors.forename: Guo-Rong
authors.initials: GR
authors.lastname: Huang
authors.affiliation: Department of General Surgery, Wuxi People's Hospital Affiliated by Nanjing Medical University No. 299, Qingyang Road, Wuxi 214023, China.
authors.forename: Dong-Dong
authors.initials: DD
authors.lastname: Ji
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D064199:Cdc20 Proteins / Q000032:analysis* / Q000235:genetics; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D016016:Proportional Hazards Models; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000401:mortality / Q000473:pathology; D013997:Time Factors; D047368:Tumor Burden
chemical_list: D014408:Biomarkers, Tumor; D064199:Cdc20 Proteins; D012333:RNA, Messenger; C577488:CDC20 protein, human
keywords: CDC20; gastric cancer; immunohistochemistry