title: Transcriptional response of immune genes in gills and the interbranchial lymphoid tissue of Atlantic salmon challenged with infectious salmon anaemia virus.
abstract: Previously, it has been assumed that fish lack organized mucosa-associated lymphoid structures. Recently, an interbranchial lymphoid tissue (ILT) was described in salmonid gills at a site with substantial exposure to antigen. In this study, immune responses were examined in gills, mid-kidney and the laser-dissected ILT of Atlantic salmon (Salmo salar L.) infected with infectious salmon anaemia virus (ISAV). A strong innate response was observed in gills and mid-kidney and even in the laser-dissected ILT, despite the fact that no virus could be traced in this tissue. A small delayed increase in IgT transcripts, exclusively in the ILT, could indicate that this tissue has a role as a secondary lymphoid organ with clonal expansion of IgT expressing B-cells. Compared to the other examined tissues, gills displayed the earliest replication of the virus, further supporting this tissue as the main entry route for infection with ISAV.
authors.affiliation: Department of Basic Science and Aquatic Medicine, The Norwegian School of Veterinary Science, Ullevålsveien 72, P.O. Box 8146 Dep, 0033 Oslo, Norway.
authors.forename: Lars
authors.initials: L
authors.lastname: Austbø
authors.affiliation: Department of Basic Science and Aquatic Medicine, The Norwegian School of Veterinary Science, Ullevålsveien 72, P.O. Box 8146 Dep, 0033 Oslo, Norway.
authors.forename: Ida Bergva
authors.initials: IB
authors.lastname: Aas
authors.affiliation: Department of Basic Science and Aquatic Medicine, The Norwegian School of Veterinary Science, Ullevålsveien 72, P.O. Box 8146 Dep, 0033 Oslo, Norway.
authors.forename: Melanie
authors.initials: M
authors.lastname: König
authors.affiliation: Norwegian Veterinary Institute, Ullevålsveien 68, P.O. Box 750 Sentrum, 0106 Oslo, Norway.
authors.forename: Simon Chioma
authors.initials: SC
authors.lastname: Weli
authors.affiliation: Department of Basic Science and Aquatic Medicine, The Norwegian School of Veterinary Science, Ullevålsveien 72, P.O. Box 8146 Dep, 0033 Oslo, Norway.
authors.forename: Mohasina
authors.initials: M
authors.lastname: Syed
authors.affiliation: Norwegian Veterinary Institute, Ullevålsveien 68, P.O. Box 750 Sentrum, 0106 Oslo, Norway.
authors.forename: Knut
authors.initials: K
authors.lastname: Falk
authors.affiliation: Department of Basic Science and Aquatic Medicine, The Norwegian School of Veterinary Science, Ullevålsveien 72, P.O. Box 8146 Dep, 0033 Oslo, Norway. Electronic address: erling.o.koppang@nmbu.no.
authors.forename: Erling Olaf
authors.initials: EO
authors.lastname: Koppang
mesh_terms: D000818:Animals; D005393:Fish Diseases / Q000235:genetics / Q000276:immunology* / Q000821:virology; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D005802:Genes, MHC Class II; D005880:Gills / Q000276:immunology* / Q000821:virology; D046829:Isavirus / Q000276:immunology*; D009976:Orthomyxoviridae Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D020317:Salmo salar / Q000235:genetics* / Q000276:immunology / Q000821:virology; D059467:Transcriptome
chemical_list: D029941:Fish Proteins
keywords: Bath challenge; ILT; ISAV; In situ hybridization; Salmo salar; qPCR