title: Comparison of spectral and image morphological analysis for egg early hatching property detection based on hyperspectral imaging.
abstract: The use of non-destructive methods to detect egg hatching properties could increase efficiency in commercial hatcheries by saving space, reducing costs, and ensuring hatching quality. For this purpose, a hyperspectral imaging system was built to detect embryo development and vitality using spectral and morphological information of hatching eggs. A total of 150 green shell eggs were used, and hyperspectral images were collected for every egg on day 0, 1, 2, 3 and 4 of incubation. After imaging, two analysis methods were developed to extract egg hatching characteristic. Firstly, hyperspectral images of samples were evaluated using Principal Component Analysis (PCA) and only one optimal band with 822 nm was selected for extracting spectral characteristics of hatching egg. Secondly, an image segmentation algorithm was applied to isolate the image morphologic characteristics of hatching egg. To investigate the applicability of spectral and image morphological analysis for detecting egg early hatching properties, Learning Vector Quantization neural network (LVQNN) was employed. The experimental results demonstrated that model using image morphological characteristics could achieve better accuracy and generalization than using spectral characteristic parameters, and the discrimination accuracy for eggs with embryo development were 97% at day 3, 100% at day 4. In addition, the recognition results for eggs with weak embryo development reached 81% at day 3, and 92% at day 4. This study suggested that image morphological analysis was a novel application of hyperspectral imaging technology to detect egg early hatching properties.
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, PR China.
authors.forename: Leiqing
authors.initials: L
authors.lastname: Pan
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, PR China.
authors.forename: Kang
authors.initials: K
authors.lastname: Tu
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: China National Research Institute of Food & Fermentation Industries, Beijing, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
mesh_terms: D000465:Algorithms; D000818:Animals; D002642:Chick Embryo; D047108:Embryonic Development / Q000502:physiology*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D016571:Neural Networks, Computer; D061848:Optical Imaging / Q000295:instrumentation* / Q000379:methods; D025341:Principal Component Analysis; D015053:Zygote / Q000254:growth & development*