title: PET imaging with multimodal upconversion nanoparticles.
abstract: A series of new upconversion nanoparticles have been functionalised with tumour-targeting molecules and metal chelates, prepared following standard peptidic and thiol chemistry. The targeting strategy has been delivered via the αvβ3 integrin, which is a heterodimeric cell surface receptor that is up-regulated in a variety of cancers, such as melanoma and breast cancer. The well-known DOTA (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid) motif allows coordination to the radionuclide (68)Ga. Radiolabelling experiments were optimised under relatively mild conditions, and are rare amongst nanoparticulate materials. In vivo application of these probes in mouse tumour models revealed their potential as specific cancer contrast agents for PET imaging.
authors.affiliation: Comprehensive Cancer Imaging Centre, Department of Surgery and Cancer, Hammersmith Campus, Imperial College London, Du Cane Road, London, W12 0NN, UK. n.long@imperial.ac.uk.
authors.forename: Juan
authors.initials: J
authors.lastname: Gallo
authors.forename: Israt S
authors.initials: IS
authors.lastname: Alam
authors.forename: Jiefu
authors.initials: J
authors.lastname: Jin
authors.forename: Yan-Juan
authors.initials: YJ
authors.lastname: Gu
authors.forename: Eric O
authors.initials: EO
authors.lastname: Aboagye
authors.forename: Wing-Tak
authors.initials: WT
authors.lastname: Wong
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Long
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000737:chemistry / Q000493:pharmacokinetics*; D005710:Gallium Radioisotopes / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000737:chemistry; D008545:Melanoma / Q000378:metabolism; D051379:Mice; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry / Q000648:ultrastructure; D009842:Oligopeptides / Q000378:metabolism; D010456:Peptides, Cyclic / Q000378:metabolism; D049268:Positron-Emission Tomography; D019275:Radiopharmaceuticals
chemical_list: D003287:Contrast Media; D005710:Gallium Radioisotopes; D006573:Heterocyclic Compounds, 1-Ring; D039302:Integrin alphaVbeta3; D009842:Oligopeptides; D010456:Peptides, Cyclic; D019275:Radiopharmaceuticals; C071349:1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid; C047981:arginyl-glycyl-aspartic acid