title: Repair of cartilage defects in BMSCs via CDMP1 gene transfection.
abstract: This study aimed at investigating the ability of cartilage-derived morphogenetic protein 1 (CDMP1) gene-transfected bone marrow mesenchymal stem cells (BMSCs) loaded on the poly(lactic-co-glycolic acid) (PLGA) scaffold for the repair of laryngeal cartilage defects and make a preliminary assessment of its repair effect. The mRNA and protein expressions of hCDMP1 were detected by reverse transcriptase-polymerase chain reaction and Western blotting. The expression of type II collagen (Col II) and glycosaminoglycan (GAG) were detected by immunohistochemistry. The cytoskeletal culture systems before and after transfection were transplanted into the rabbit full-thickness defects of thyroid cartilage for observation of the repair of cartilage defects from general and histological aspects. The exogenous hCDMP1 gene could be successfully transplanted into BMSCs through adenovirus infection to obtain a stable expression. Compared with the control group, hCDMP1 gene-transfected BMSCs had enhanced secretory abilities of Col II, GAG, and other cartilage-specific matrices, with a trend of promoting cartilage differentiation. The transfected cytoskeletal complexes could more effectively repair laryngeal cartilage defects. hCDMP1 gene-transfected BMSCs/PLGA 3-D biological scaffold compounds transplanted into animal bodies could effectively repair laryngeal cartilage defects.
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning Province, China.
authors.forename: G
authors.initials: G
authors.lastname: Wu
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning Province, China yingcuicn@126.com.
authors.forename: Y
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning Province, China.
authors.forename: Y T
authors.initials: YT
authors.lastname: Wang
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning Province, China.
authors.forename: M
authors.initials: M
authors.lastname: Yao
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning Province, China.
authors.forename: J
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning Province, China.
authors.forename: J X
authors.initials: JX
authors.lastname: Li
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning Province, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning Province, China.
authors.forename: B
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D055428:Growth Differentiation Factor 5 / Q000235:genetics / Q000378:metabolism*; D048091:Guided Tissue Regeneration; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D012038:Regeneration; D013957:Thyroid Cartilage / Q000502:physiology* / Q000601:surgery; D054457:Tissue Scaffolds / Q000737:chemistry; D014162:Transfection
chemical_list: D024043:Collagen Type II; C525893:GDF5 protein, human; D006025:Glycosaminoglycans; D055428:Growth Differentiation Factor 5; D012333:RNA, Messenger