title: Role of F1C fimbriae, flagella, and secreted bacterial components in the inhibitory effect of probiotic Escherichia coli Nissle 1917 on atypical enteropathogenic E. coli infection.
abstract: Enteropathogenic Escherichia coli (EPEC) is recognized as an important intestinal pathogen that frequently causes acute and persistent diarrhea in humans and animals. The use of probiotic bacteria to prevent diarrhea is gaining increasing interest. The probiotic E. coli strain Nissle 1917 (EcN) is known to be effective in the treatment of several gastrointestinal disorders. While both in vitro and in vivo studies have described strong inhibitory effects of EcN on enteropathogenic bacteria, including pathogenic E. coli, the underlying molecular mechanisms remain largely unknown. In this study, we examined the inhibitory effect of EcN on infections of porcine intestinal epithelial cells with atypical enteropathogenic E. coli (aEPEC) with respect to single infection steps, including adhesion, microcolony formation, and the attaching and effacing phenotype. We show that EcN drastically reduced the infection efficiencies of aEPEC by inhibiting bacterial adhesion and growth of microcolonies, but not the attaching and effacing of adherent bacteria. The inhibitory effect correlated with EcN adhesion capacities and was predominantly mediated by F1C fimbriae, but also by H1 flagella, which served as bridges between EcN cells. Furthermore, EcN seemed to interfere with the initial adhesion of aEPEC to host cells by secretion of inhibitory components. These components do not appear to be specific to EcN, but we propose that the strong adhesion capacities enable EcN to secrete sufficient local concentrations of the inhibitory factors. The results of this study are consistent with a mode of action whereby EcN inhibits secretion of virulence-associated proteins of EPEC, but not their expression.
authors.affiliation: Zentrum für Infektionsmedizin, Institut für Mikrobiologie und Tierseuchen, Fachbereich Veterinärmedizin, Freie Universität Berlin, Berlin, Germany.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Kleta
authors.forename: Marcel
authors.initials: M
authors.lastname: Nordhoff
authors.forename: Karsten
authors.initials: K
authors.lastname: Tedin
authors.forename: Lothar H
authors.initials: LH
authors.lastname: Wieler
authors.forename: Rafal
authors.initials: R
authors.lastname: Kolenda
authors.forename: Sibylle
authors.initials: S
authors.lastname: Oswald
authors.forename: Tobias A
authors.initials: TA
authors.lastname: Oelschlaeger
authors.forename: Wilfried
authors.initials: W
authors.lastname: Bleiss
authors.forename: Peter
authors.initials: P
authors.lastname: Schierack
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D002460:Cell Line; D054308:Enteropathogenic Escherichia coli / Q000472:pathogenicity / Q000502:physiology* / Q000648:ultrastructure; D004847:Epithelial Cells / Q000382:microbiology; D004926:Escherichia coli / Q000145:classification*; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D036781:Fimbriae Proteins / Q000235:genetics / Q000378:metabolism*; D005407:Flagella / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D007413:Intestinal Mucosa / Q000166:cytology; D019936:Probiotics / Q000494:pharmacology*; D013552:Swine; D014774:Virulence
chemical_list: D029968:Escherichia coli Proteins; D036781:Fimbriae Proteins