title: Serum S100B level increases after running but not cycling exercise.
abstract: The objective of this study was to investigate the effect of running versus cycling exercises upon serum S100B levels and typical markers of skeletal muscle damage such as creatine kinase (CK), aspartate aminotransferase (AST) and myoglobin (Mb). Although recent work demonstrates that S100B is highly expressed and exerts functional properties in skeletal muscle, there is no previous study that tries to establish a relationship between muscle damage and serum S100B levels after exercise. We conducted a cross-sectional study on 13 male triathletes. They completed 2 submaximal exercise protocols at anaerobic threshold intensity. Running was performed on a treadmill with no inclination (RUN) and cycling (CYC) using a cycle-simulator. Three blood samples were taken before (PRE), immediately after (POST) and 1 h after exercise for CK, AST, Mb and S100B assessments. We found a significant increase in serum S100B levels and muscle damage markers in RUN POST compared with RUN PRE. Comparing groups, POST S100B, CK, AST and Mb serum levels were higher in RUN than CYC. Only in RUN, the area under the curve (AUC) of serum S100B is positively correlated with AUC of CK and Mb. Therefore, immediately after an intense exercise such as running, but not cycling, serum levels of S100B protein increase in parallel with levels of CK, AST and Mb. Additionally, the positive correlation between S100B and CK and Mb points to S100B as an acute biomarker of muscle damage after running exercise.
authors.affiliation: a Federal Institute of Education, Science and Technology of Rio Grande do Sul, Porto Alegre RS, Brazil.
authors.forename: Cintia Mussi Alvim
authors.initials: CM
authors.lastname: Stocchero
authors.forename: Jean Pierre
authors.initials: JP
authors.lastname: Oses
authors.forename: Giovani Santos
authors.initials: GS
authors.lastname: Cunha
authors.forename: Jocelito Bijoldo
authors.initials: JB
authors.lastname: Martins
authors.forename: Liz Marina
authors.initials: LM
authors.lastname: Brum
authors.forename: Eduardo Rigon
authors.initials: ER
authors.lastname: Zimmer
authors.forename: Diogo Onofre
authors.initials: DO
authors.lastname: Souza
authors.forename: Luis Valmor
authors.initials: LV
authors.lastname: Portela
authors.forename: Alvaro
authors.initials: A
authors.lastname: Reischak-Oliveira
mesh_terms: D000328:Adult; D001642:Bicycling / Q000502:physiology*; D015415:Biomarkers / Q000097:blood; D003430:Cross-Sectional Studies; D015444:Exercise / Q000502:physiology; D006801:Humans; D008297:Male; D012420:Running / Q000502:physiology*; D064568:S100 Calcium Binding Protein beta Subunit / Q000097:blood*
chemical_list: D015415:Biomarkers; D064568:S100 Calcium Binding Protein beta Subunit; C579160:S100B protein, human