title: Serum miR-499 as a novel diagnostic and prognostic biomarker in non-small cell lung cancer.
abstract: The aim of the present study was to determine whether serum miR-499 may be used as a biomarker for early detection of non-small cell lung cancer (NSCLC). The present study was designed as an initial screening phase and a subsequent validation phase. In the screening phase, we analyzed serum levels of miR-499 in a subset of 40 patients with stage I (n=20) and stage IV (n=20) NSCLC. In the validation phase, miR-499 expression levels in serum (n=514) and tissue (n=136) from NSCLC patients were detected in a large and independent cohort of 514 patients. miR-499 in the screening phase was found to be significantly elevated in the serum of stage I NSCLC patients compared with that in stage IV NSCLC patients (P<0.001). Validation analysis showed that serum miR-499 levels were robust in differentiating NSCLC patients from control subjects [area under the curve (AUC)=0.906; 95% confidence interval (CI)=0.879 to 0.929). Serum miR-499 levels were significantly lower in stage III and IV patients compared with those with stage I (both P<0.001) or II (both P<0.001). Low serum miR-499 levels were associated with shorter overall survival and served as an independent prognostic biomarker in NSCLC patients [hazard ratio (HR)=1.63; 95% CI=1.33-2.0; P<0.0001). In addition, low serum levels of miR-499 indicated a poor disease-free survival in stage I-II NSCLC patients. Serum miR-499 may prove to be a promising biomarker for early detection and prognosis prediction of NSCLC.
authors.affiliation: Department of Respiratory Medicine, Shanghai 10th People's Hospital, Tongji University, Shanghai 200072, P.R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Nephrology, Huashan Hospital, Fudan University, Shanghai 200032, P.R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University, Shanghai 200433, P.R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Nuclear Medicine, Shanghai 10th People's Hospital, Tongji University, Shanghai 200072, P.R. China.
authors.forename: Chengyou
authors.initials: C
authors.lastname: Jia
authors.affiliation: Department of Neural Medicine, People's Hospital of Zunhua County, Tangshan, Hebei 064200, P.R. China.
authors.forename: Fuhua
authors.initials: F
authors.lastname: Shi
authors.affiliation: Department of Cardiology, People's Hospital of Zunhua County, Tangshan, Hebei 064200, P.R. China.
authors.forename: Shaocai
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Respiratory Medicine, Shanghai 10th People's Hospital, Tongji University, Shanghai 200072, P.R. China.
authors.forename: Aimei
authors.initials: A
authors.lastname: Peng
authors.affiliation: Department of Respiratory Medicine, Shanghai 10th People's Hospital, Tongji University, Shanghai 200072, P.R. China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, Shanghai 10th People's Hospital, Tongji University, Shanghai 200072, P.R. China.
authors.forename: Xiaolian
authors.initials: X
authors.lastname: Song
authors.affiliation: Department of Respiratory Medicine, Shanghai 10th People's Hospital, Tongji University, Shanghai 200072, P.R. China.
authors.forename: Changhui
authors.initials: C
authors.lastname: Wang
mesh_terms: D000368:Aged; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood* / Q000175:diagnosis* / Q000401:mortality; D055088:Early Detection of Cancer / Q000379:methods; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000097:blood* / Q000175:diagnosis* / Q000401:mortality; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D011379:Prognosis; D012372:ROC Curve; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; C548683:MIRN499 microRNA, human; D035683:MicroRNAs