title: Decrease of NH4+-N by bacterioplankton accelerated the removal of cyanobacterial blooms in aerated aquatic ecosystem.
abstract: We used aerated systems to assess the influence of the bacterioplankton community on cyanobacterial blooms in algae/post-bloom of Lake Taihu, China. Bacterioplankton community diversity was evaluated by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) fingerprinting. Chemical analysis and nitrogen dynamic changes illustrated that NH4+-N was nitrified to NO2--N and NO3--N by bacterioplankton. Finally, NH4+-N was exhausted and NO3--N was denitrified to NO2--N, while the accumulation of NO2--N indicated that bacterioplankton with completely aerobic denitrification ability were lacking in the water samples collected from Lake Taihu. We suggested that adding completely aerobic denitrification bacteria (to denitrify NO2--N to N2) would improve the water quality. PCR-DGGE and sequencing results showed that more than1/3 of the bacterial species were associated with the removal of nitrogen, and Acidovorax temperans was the dominant one. PCR-DGGE, variation of nitrogen, removal efficiencies of chlorophyll-a and canonical correspondence analysis indicated that the bacterioplanktonsignificantly influenced the physiological and biochemical changes of cyanobacteria. Additionally, the unweighted pair-group method with arithmetic means revealed there was no obvious harm to the microecosystem from aeration. The present study demonstrated that bacterioplankton can play crucial roles in aerated ecosystems, which could control the impact of cyanobacterial blooms in eutrophicated fresh water systems.
authors.affiliation: Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China. yangxi0414@gmail.com
authors.forename: Xi
authors.initials: X
authors.lastname: Yang
authors.affiliation: Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Xie
authors.affiliation: Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Zhimei
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Huihui
authors.initials: H
authors.lastname: Fan
authors.affiliation: Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Shen
mesh_terms: D064751:Ammonium Compounds / Q000737:chemistry*; D002681:China; D000458:Cyanobacteria / Q000378:metabolism*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D017753:Ecosystem; D005068:Eutrophication / Q000502:physiology*; D060106:Lakes; D009566:Nitrates / Q000737:chemistry / Q000378:metabolism; D016133:Polymerase Chain Reaction
chemical_list: D064751:Ammonium Compounds; D004269:DNA, Bacterial; D009566:Nitrates