title: Preparation of a stir bar coated with molecularly imprinted polymer and its application in analysis of dopamine in urine.
abstract: A molecularly imprinted polymer coated stir bar (MIP-SB) using dopamine as a template was fabricated. The adsorptive capacity of the MIP-SB was almost 4 times over that of non-imprinted stir bar. The MIP-SB showed good extracting selectivity for dopamine when used to adsorb dopamine and its analogs. An analytical method to determine dopamine in urine sample was established by combining MIP-SB sorptive extraction with HPLC-fluorescence detector. The linear range of dopamine concentration was 0.378-18.9ng/ml with correlation coefficient of 0.9990, and the limit of quantification was about 0.0945ng/ml (S/N=10). The recoveries of dopamine with spiked urine samples at three different levels were between 92.3 and 106.9%, and the relative standard deviations were within 13.2% (n=3). The method is simple and suitable for the determination of dopamine in human urine for clinical application.
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu 211166, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Lei
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu 211166, PR China.
authors.forename: Guanhong
authors.initials: G
authors.lastname: Xu
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu 211166, PR China.
authors.forename: Fangdi
authors.initials: F
authors.lastname: Wei
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu 211166, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu 211166, PR China. Electronic address: huqin@njmu.edu.cn.
authors.forename: Qin
authors.initials: Q
authors.lastname: Hu
mesh_terms: D000327:Adsorption; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004298:Dopamine / Q000737:chemistry* / Q000652:urine*; D006801:Humans; D057230:Limit of Detection; D054802:Molecular Imprinting / Q000379:methods; D011108:Polymers / Q000737:chemistry*
chemical_list: D011108:Polymers; D004298:Dopamine
keywords: Dopamine; Human urine; Molecularly imprinted polymer; Stir bar sorptive extraction