title: Indole-3-acetic acid reverses the harpin-induced hypersensitive response and alters the expression of hypersensitive-response-related genes in tobacco.
abstract: Harpin proteins stimulate hypersensitive response (HR) in plants. However, the mechanism by which HR is regulated is not clear. The role of the auxin, indole-3-acetic acid (IAA), in the control of harpin-stimulated HR was investigated. IAA was used to inhibit HR that was stimulated by purified fusion harpin(Xoo) protein in tobacco. Semi-quantitative PCR and qRT-PCR were employed to detect the expression of HR related genes. IAA at 100 μM reversed harpin-induced HR which was inhibited by 500 μM 2,3,5-triiodobenzoic acid (TIBA). Semi-quantitative PCR and qRT-PCR showed the combined application of 100 μM IAA and harpin protein from Xanthomonas oryzae enhanced the expression of HR marker gene, hsr203J, but weakened the expression of the disease-defense gene, chia5. TIBA also decreased the expression of hsr203J but increased the expression of chia5. Thus, the auxin can reverse harpin(Xoo)-induced HR.
authors.affiliation: Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource/College of Environment and Plant Protection, Hainan University, Haikou, 570228, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Song
authors.forename: Xiao-chong
authors.initials: XC
authors.lastname: Gong
authors.forename: Wei-guo
authors.initials: WG
authors.lastname: Miao
authors.forename: Fu-cong
authors.initials: FC
authors.lastname: Zheng
authors.forename: Cong-feng
authors.initials: CF
authors.lastname: Song
authors.forename: Ming-hua
authors.initials: MH
authors.lastname: Wang
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D007210:Indoleacetic Acids / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014283:Triiodobenzoic Acids / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D007210:Indoleacetic Acids; D010940:Plant Proteins; D014283:Triiodobenzoic Acids; C030737:indoleacetic acid; C052968:2,3,5-triiodobenzoic acid