title: Bioelectrochemical systems with oleylamine-stabilized gold nanostructures and horseradish peroxidase for hydrogen peroxide sensor.
abstract: This paper describes ultrathin gold nanowires (NWs) and nanoparticles (NPs) prepared by oleylamine (OA) synthesis and their assembly with horseradish peroxidase enzyme (HRP) for bioelectrochemical sensing of hydrogen peroxide for the first time. The immobilization of oxidoreductase enzyme HRP on the electrodes modified with OA gold nanostructures (OANSs) is discussed. The HRP-sensor characteristics, namely sensitivity, working concentration range, sensor-to-sensor and measurement-to-measurement reproducibility as well as long-term stability, are improved significantly compared to the planar thin-film sensors by using OANSs. The thin-film gold electrodes modified with OANWs and OANPs exhibit a catalytic activity towards oxidation of hydrogen peroxide with a working concentration range from 20 µM to 500 µM, a sensitivity of 0.031 A M(-1) cm(-2) (RSD 0.046) and 0.027 A M(-1) cm(-2) (RSD 0.045), and a detection limit of 5 µM and 8 µM, respectively (RSD near the detection limits was 9-12%). Our study shows that ultrathin gold nanowires and nanoparticles prepared by oleylamine synthesis are prospective materials to assemble biomolecules into functional nanoarchitectures for enzyme-based bioelectrochemical sensors, metalloprotein bioelectronics, and energy research.
authors.affiliation: Faculty of Chemistry, St. Petersburg State University, Universitetskaya nab. 7/9, 199034 St. Petersburg, Russia.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Koposova
authors.affiliation: Peter Grünberg Institute 8, Forschungszentrum Jülich GmbH, 52428 Jülich, Germany; Jülich-Aachen Research Alliance - Fundamentals of Future Information Technology (JARA-FIT), Germany.
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.affiliation: Peter Grünberg Institute 8, Forschungszentrum Jülich GmbH, 52428 Jülich, Germany; Jülich-Aachen Research Alliance - Fundamentals of Future Information Technology (JARA-FIT), Germany.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Kisner
authors.affiliation: Faculty of Chemistry, St. Petersburg State University, Universitetskaya nab. 7/9, 199034 St. Petersburg, Russia.
authors.forename: Yury
authors.initials: Y
authors.lastname: Ermolenko
authors.affiliation: Faculty of Chemistry, St. Petersburg State University, Universitetskaya nab. 7/9, 199034 St. Petersburg, Russia.
authors.forename: Galina
authors.initials: G
authors.lastname: Shumilova
authors.affiliation: Peter Grünberg Institute 8, Forschungszentrum Jülich GmbH, 52428 Jülich, Germany; Jülich-Aachen Research Alliance - Fundamentals of Future Information Technology (JARA-FIT), Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Offenhäusser
authors.affiliation: Peter Grünberg Institute 8, Forschungszentrum Jülich GmbH, 52428 Jülich, Germany; Jülich-Aachen Research Alliance - Fundamentals of Future Information Technology (JARA-FIT), Germany. Electronic address: y.mourzina@fz-juelich.de.
authors.forename: Yulia
authors.initials: Y
authors.lastname: Mourzina
mesh_terms: D000588:Amines / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D055664:Electrochemical Techniques / Q000295:instrumentation; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000378:metabolism; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry*; D053770:Nanowires / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D000588:Amines; D004800:Enzymes, Immobilized; D006046:Gold; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; C008703:oleylamine
keywords: Biosensor; Cyclic voltammetry; Gold nanoparticle; Gold nanowire; Horseradish peroxidase