title: Development of a direct competitive ELISA for the detection of Mycoplasma bovis infection based on a monoclonal antibody of P48 protein.
abstract: BACKGROUND
  Mycoplasma bovis (M. bovis) is a major, but often overlooked, pathogen documented to cause respiratory disease, mastitis, and arthritis in cattle throughout China since 2008. Here, we report the development of a direct competitive enzyme-linked immunosorbent assay (Dc-ELISA) to detect M. bovis antibody.
  
  
  RESULTS
  We used a recombinant P48 protein and monoclonal antibody (mAb) 10E. MAb 10E, prepared against the recombinant P48 protein of M. bovis, identified all M. bovis strains with no cross-reactivity with other related pathogens. Coating micro plates with P48 protein instead of whole M. bovis cells as well as the use of mAb 10E produced a specific and sensitive Dc-ELISA for M. bovis antibody detection with a cut-off percent inhibition (PI) value of 32%. Compared with two commercial indirect ELISA (i-ELISA) kits, our Dc-ELISA offered a higher positive detection rate in 165 clinical bovine serum samples.
  
  
  CONCLUSIONS
  A rapid, sensitive, and reliable serological diagnosis method was developed for M. bovis, which can facilitate M. bovis surveillance, assisting researchers in understanding the ecology and epidemiology of M. bovis.
authors.forename: Ping
authors.initials: P
authors.lastname: Fu
authors.forename: Zhenhong
authors.initials: Z
authors.lastname: Sun
authors.forename: Yuewei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ziqiang
authors.initials: Z
authors.lastname: Yu
authors.forename: Haiyan
authors.initials: H
authors.lastname: Zhang
authors.forename: Dan
authors.initials: D
authors.lastname: Su
authors.forename: Fei
authors.initials: F
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China. wuwenxue@cau.edu.cn.
authors.forename: Wenxue
authors.initials: W
authors.lastname: Wu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins / Q000276:immunology / Q000378:metabolism*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000662:veterinary*; D045682:Mycoplasma bovis / Q000302:isolation & purification* / Q000378:metabolism; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods / Q000662:veterinary
chemical_list: D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins