title: Mutations and chromosomal aberrations in hMTH1-transfected and non-transfected TK6 cells after exposure to low dose rates of gamma radiation.
abstract: The aim of the present study was to analyse the dose rate effect of gamma radiation at the level of mutations, chromosomal aberrations, and cell growth in TK6 cells with normal as well as reduced levels of hMTH1 protein. TK6 cells were exposed to gamma radiation at dose rates ranging from 1.4 to 30.0 mGy/h (chronic exposure) as well as 24 Gy/h (acute exposure). Cell growth, frequency of thymidine kinase mutants, and of chromosomal aberrations in painted chromosomes 2, 8, and 14 were analysed. A decline in cell growth and an increase in unstable-type chromosomal aberrations with increasing dose rate were observed in both cell lines. A dose rate effect was not seen on mutations or stable-type chromosomal aberrations in any of the two cell lines. Reduction in the hMTH1 protein does not influence the sensitivity of TK6 cells to gamma radiation. This result fits well with data of others generated with the same cell line.
authors.affiliation: Department of Molecular Biosciences, Centre for Radiation Protection Research (CRPR), The Wenner-Gren Institute, Stockholm University, 106 91, Stockholm, Sweden.
authors.forename: Sara Shakeri
authors.initials: SS
authors.lastname: Manesh
authors.forename: Marta
authors.initials: M
authors.lastname: Deperas-Kaminska
authors.forename: Asal
authors.initials: A
authors.lastname: Fotouhi
authors.forename: Traimate
authors.initials: T
authors.lastname: Sangsuwan
authors.forename: Mats
authors.initials: M
authors.lastname: Harms-Ringdahl
authors.forename: Andrzej
authors.initials: A
authors.lastname: Wojcik
authors.forename: Siamak
authors.initials: S
authors.lastname: Haghdoost
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D002869:Chromosome Aberrations / Q000528:radiation effects*; D002999:Clone Cells / Q000166:cytology / Q000528:radiation effects; D045643:DNA Repair Enzymes / Q000235:genetics*; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays / Q000009:adverse effects*; D006801:Humans; D009154:Mutation / Q000528:radiation effects*; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics*; D011829:Radiation Dosage; D014162:Transfection
chemical_list: D010744:Phosphoric Monoester Hydrolases; C078207:8-oxodGTPase; D045643:DNA Repair Enzymes