title: An ionic liquid tolerant cellulase derived from chemically polluted microhabitats and its application in in situ saccharification of rice straw.
abstract: A cellulase-producing fungus was isolated from chemically polluted microhabitats by [Amim][Cl] enrichment and identified as Aspergillus fumigatus. The maximum activity of the cellulase in 30% (v/v) ionic liquids (ILs) was detected in [Emim][DMP], [Amim][Cl] and [Emim][MA] as 127%, 111% and 109%, respectively, of its activity in buffer, suggesting its superior performance in high concentration ILs. Strikingly, although its initial activity varied in each IL, its half-life was longer in most ILs than in buffer, evidence of a high conformational stability of the enzyme that is essential for maintaining the remaining activity in relevant media. It noteworthy that 1-3M NaCl can activate the cellulase somewhat. More gratifyingly, a compatible IL-cellulase system based on the cellulase was developed, and its use significantly improved the saccharification rate of rice straw from 53% to 88% versus the control, demonstrating its potential for efficient transformation of lignocellulose to glucose in a single-step process.
authors.affiliation: Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, School of Chemistry and Chemical Engineering, Huaiyin Normal University, Huaian 223300, China. Electronic address: xujiaxing@hytc.edu.cn.
authors.forename: Jiaxing
authors.initials: J
authors.lastname: Xu
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, 30 Puzhunan Road, Nanjing 210000, China.
authors.forename: Bingfang
authors.initials: B
authors.lastname: He
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, 30 Puzhunan Road, Nanjing 210000, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, 30 Puzhunan Road, Nanjing 210000, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: College of Life Science and Technology, Guangxi University, Nanning, Guangxi 530004, China.
authors.forename: Chenghua
authors.initials: C
authors.lastname: Wang
authors.affiliation: Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, School of Chemistry and Chemical Engineering, Huaiyin Normal University, Huaian 223300, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Hu
mesh_terms: D001232:Aspergillus fumigatus / Q000201:enzymology; D050260:Carbohydrate Metabolism / Q000187:drug effects*; D002480:Cellulase / Q000378:metabolism*; D017753:Ecosystem; D004787:Environmental Pollution; D004795:Enzyme Stability / Q000187:drug effects; D006207:Half-Life; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D052578:Ionic Liquids / Q000494:pharmacology*; D012275:Oryza / Q000737:chemistry*; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors; D014866:Waste Products
chemical_list: D052578:Ionic Liquids; D014866:Waste Products; D012965:Sodium Chloride; D002480:Cellulase
keywords: Cellulase; Ionic liquids; Lignocellulose; Saccharification