title: [Effects of high plating densities on in vitro and in vivo differentiation capability of immortalized human mesenchymal stem cells].
abstract: OBJECTIVE
  To determine whether different cell plating densities could influence the potential differentiation of immortalized human mesenchymal stem cells (hMSC-TERT).
  
  
  METHODS
  Extensive characterization was performed for two independent cell lines derived from parental hMSC-TERT cell line based on different plating densities during expansion in culture: 1: 2 (hMSC-TERT2) and 1: 20 (hMSC-TERT20). Their adipocytic, osteogenic, neuronal differentiation potential in vitro and multidirectional differentiation potential in vivo were analyzed by immunohistochemistry for pathologic tissue markers.
  
  
  RESULTS
  hMSC-TERT2 formed cellular adipose foci and mineralized tuberculum after in vitro induction while hMSC-TERT20 showed adipocytes but no adipose foci or mineralized tuberculum. The positive rate of hMSC-TERT2 formed tissues on VIM, GFAP and LCA (89.17% ± 5.97%, 72.5% ± 10.11% and 76.67% ± 11.15%) under SCID murine skin were more than that of hMSC-TERT20 formed tissues (0.33% ± 0.65%, 9.58% ± 4.29% and 22.08% ± 6.95%).
  
  
  CONCLUSIONS
  The cell lines derived at high plating density may have more powerful differentiation potential in vitro and in vivo than those derived at low cell plating density.
authors.affiliation: Department of Neurosurgery, Hebei United University Affiliated Hospital, Tangshan 063000, China.
authors.forename: Ze-lin
authors.initials: ZL
authors.lastname: Sun
authors.affiliation: Beijing Neurosurgical Institute, Beijing 100050, China. Email: tsszl666@163.com.
authors.forename: Ya-zhuo
authors.initials: YZ
authors.lastname: Zhang
mesh_terms: D017667:Adipocytes / Q000166:cytology; D001854:Bone Marrow Cells / Q000166:cytology*; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002460:Cell Line; D006801:Humans; D010006:Osteoblasts / Q000166:cytology