title: Multiple effects of Escherichia coli Nissle 1917 on growth, biofilm formation, and inflammation cytokines profile of Clostridium perfringens type A strain CP4.
abstract: Clostridium perfringens is an important Gram-positive pathogen responsible for food poisoning, necrotic enteritis, gas gangrene, and even death. Escherichia coli Nissle 1917 (EcN) is a well-characterized probiotic strain with demonstrated benefits. In this study, we evaluated the effects of EcN on growth, toxin production, biofilm formation, and inflammatory cytokine responses of C. perfringens. In vitro co-culture experiments demonstrated that EcN inhibited growth, gas production, and toxin production (α-toxin and NetB) of C. perfringens in a dose-dependent manner. The growth inhibition effect was not observed when C. perfringens was incubated with EcN cell-free supernatants (CFSE), suggesting that growth inhibition was caused by nutrition competition during co-incubation. In vitro studies demonstrated that pre-incubation with EcN did not inhibit C. perfringens attachment to Caco-2 cells, but did reduce C. perfringens total number, toxin production, and cytotoxicity after 24 h. The similar growth inhibition results were also observed during the formation of C. perfringens biofilm. Finally, pre-incubation of EcN with RAW264.7 cells significantly decreased the production of inflammatory cytokines caused by the introduction of C. perfringens. Our results indicate that EcN can inhibit many of the pathological effects of C. perfringens in vitro conditions.
authors.affiliation: Center for Infectious Diseases and Vaccinology, The Biodesign Institute, Arizona State University, Tempe, AZ, USA.
authors.forename: Yanlong
authors.initials: Y
authors.lastname: Jiang
authors.forename: Qingke
authors.initials: Q
authors.lastname: Kong
authors.forename: Kenneth L
authors.initials: KL
authors.lastname: Roland
authors.forename: Amanda
authors.initials: A
authors.lastname: Wolf
authors.forename: Roy
authors.initials: R
authors.lastname: Curtiss
mesh_terms: D000818:Animals; D000898:Antibiosis; D018441:Biofilms / Q000254:growth & development*; D002460:Cell Line; D003015:Clostridium Infections / Q000378:metabolism* / Q000382:microbiology*; D003016:Clostridium perfringens / Q000502:physiology*; D016207:Cytokines / Q000378:metabolism*; D004926:Escherichia coli / Q000502:physiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D008264:Macrophages / Q000378:metabolism / Q000382:microbiology; D051379:Mice; D056265:Microbial Interactions; D019936:Probiotics
chemical_list: D016207:Cytokines
keywords: C. perfringens; E. coli Nissle 1917; biofilm; cytokine; inhibition; toxin