title: Role of bone-type tissue-nonspecific alkaline phosphatase and PHOSPO1 in vascular calcification.
abstract: Matrix vesicle (MV)-mediated mineralization is important for bone ossification. However, under certain circumstances such as atherosclerosis, mineralization may occur in the arterial wall. Bone-type tissue-nonspecific alkaline phosphatase (TNAP) hydrolyzes inorganic pyrophosphate (PPi) and generates inorganic phosphate (Pi), which is essential for MV-mediated hydroxyapatite formation. MVs contain another phosphatase, PHOSPHO1, that serves as an additional supplier of Pi. Activation of bone-type tissue-nonspecific alkaline phosphatase (TNAP) in vascular smooth muscle cells precedes vascular calcification. By degrading PPi, TNAP plays a procalcific role changing the Pi/PPi ratio toward mineralization. A pathologic role of bone-type TNAP and PHOSPHO1 make them to be attractive targets for cardiovascular therapy.
authors.forename: Yuri V
authors.initials: YV
authors.lastname: Bobryshev
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Orekhov
authors.forename: Igor
authors.initials: I
authors.lastname: Sobenin
authors.affiliation: Department of Medical Nanobiotechnology, Pirogov Russian State Medical University, 1 Ostrovityanova Str., 117997 Moscow, Russia. dimitry.chistiakov@lycos.com.
authors.forename: Dimitry A
authors.initials: DA
authors.lastname: Chistiakov
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D001842:Bone and Bones / Q000201:enzymology* / Q000378:metabolism*; D006801:Humans; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D061205:Vascular Calcification / Q000378:metabolism*
chemical_list: C497128:PHOSPHO1 protein, human; C548267:ALPL protein, human; D000469:Alkaline Phosphatase; D010744:Phosphoric Monoester Hydrolases