title: Herbs in association with drugs: Enhancement of the aminoglycoside-antibiotic activity by Pityrogramma calomelanos (L.) Link.
abstract: OBJECTIVE
  In this work, the ethyl acetate and hexane fractions of Pityrogramma calomelanos (L.) were evaluated to antibacterial and antifungal activity against strains of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans, Candida krusei and Candida tropicalis.
  
  
  METHODS
  The study was performed aiming to assess the antimicrobial effect with the method of dilution in HIA. The hexane and ethyl acetate fractions were named HFPC and EAFPC, respectively.
  
  
  RESULTS
  Both fractions of specie P. calomelanos displayed good activity against S. aureus when associated with gentamicin. When associated with the antifungal, the fractions did not exhibit relevant activity against species of Candida.
  
  
  CONCLUSIONS
  These results indicate that this specie can be used as a possible source of natural products of antibacterial interest, mainly when combined with aminoglycosides.
authors.affiliation: Laboratory of Microbiology and Molecular Biology, Department of Biological Chemistry, University of the Region of Cariri, CE/Crato, CE, Brazil.
authors.forename: Teógenes M
authors.initials: TM
authors.lastname: Souza
authors.affiliation: Laboratory of Microbiology and Molecular Biology, Department of Biological Chemistry, University of the Region of Cariri, CE/Crato, CE, Brazil.
authors.forename: Maria F B
authors.initials: MF
authors.lastname: Morais-Braga
authors.affiliation: Laboratory of Natural Products Research, Department of Biological Chemistry, University of the Region of Cariri, CE/Crato, Brazil.
authors.forename: José G M
authors.initials: JG
authors.lastname: Costa
authors.affiliation: Laboratory of Paleontology, Department of Biological Sciences, University of the Region of Cariri, CE/Crato, Brazil.
authors.forename: Antônio A F
authors.initials: AA
authors.lastname: Saraiva
authors.affiliation: Laboratory of Biochemistry and Cellular Biology, Department of Ecology and Environment, Federal University of Paraiba, PB/Rio Tinto, Brazil.
authors.forename: Micheline A
authors.initials: MA
authors.lastname: Lima
authors.affiliation: Laboratory of Microbiology and Molecular Biology, Department of Biological Chemistry, University of the Region of Cariri, CE/Crato, CE, Brazil.
authors.forename: Henrique D M
authors.initials: HD
authors.lastname: Coutinho
keywords: Antimicrobial activity; Ethyl acetate fraction; Hexane fraction; Modifying antibiotic activity; Pityrogramma calomelanos