title: Enzyme-etching technique to fabricate micropatterns of aligned collagen fibrils.
abstract: A technique to tailor-make pre-coated, pre-aligned bovine collagen fibrils, derived from neonatal cardiomyocytes, on the surface of a glass slide into a designated pattern is reported. The unwanted collagen-coated area was erased by a collagenase solution and the tailored area was retained by attaching a microfabricated polydimethylsiloxane stamp directly to the collagen-coated surface. Using this technique, collagen patterns with designated orientations and with clear pattern boundaries and defined shapes were fabricated.
authors.affiliation: Department of Bioengineering, Clemson University, Clemson, SC, 29634, USA.
authors.forename: Honghai
authors.initials: H
authors.lastname: Liu
authors.forename: Ruikai
authors.initials: R
authors.lastname: Chen
authors.forename: Huaxiao
authors.initials: H
authors.lastname: Yang
authors.forename: Wan
authors.initials: W
authors.lastname: Qin
authors.forename: Thomas K
authors.initials: TK
authors.lastname: Borg
authors.forename: Delphine
authors.initials: D
authors.lastname: Dean
authors.forename: Meifeng
authors.initials: M
authors.lastname: Xu
authors.forename: Bruce Z
authors.initials: BZ
authors.lastname: Gao
mesh_terms: D020099:Coated Materials, Biocompatible; D003094:Collagen / Q000378:metabolism*; D017364:Collagenases / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D055616:Microtechnology / Q000379:methods*; D032383:Myocytes, Cardiac / Q000737:chemistry
chemical_list: D020099:Coated Materials, Biocompatible; D003094:Collagen; D017364:Collagenases