title: Triclocarban-induced change in intracellular Ca²⁺ level in rat thymocytes: cytometric analysis with Fluo-3 under Zn²⁺-free conditions.
abstract: Triclocarban (TCC) is an antimicrobial used in personal hygiene products. Recent health concerns arose after TCC was detected in the blood of human subjects who showered with soap containing TCC. In this study, the effect of TCC on intracellular Ca(2+) concentration in rat thymocytes was examined using Fluo-3, an indicator of intracellular Ca(2+). TCC at concentrations ranging from 0.1 μM to 3 μM increased intracellular Ca(2+) concentration biphasically: first by releasing Ca(2+) from intracellular Ca(2+) stores and then inducing Ca(2+) influx through store-operated Ca(2+) channels. The threshold TCC concentration to increase intracellular Ca(2+) concentration in this study was lower than the maximum TCC concentrations reported in human blood samples. Therefore, we anticipate that TCC at concentrations reported in human blood samples might disturb intracellular Ca(2+) signaling in human lymphocytes.
authors.affiliation: Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima, Tokushima 770-8502, Japan.
authors.forename: Yukari
authors.initials: Y
authors.lastname: Miura
authors.affiliation: Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima, Tokushima 770-8502, Japan.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Chen
authors.affiliation: Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima, Tokushima 770-8502, Japan.
authors.forename: Saki
authors.initials: S
authors.lastname: Yamada
authors.affiliation: Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima, Tokushima 770-8502, Japan.
authors.forename: Aya
authors.initials: A
authors.lastname: Sugihara
authors.affiliation: Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima, Tokushima 770-8502, Japan.
authors.forename: Molomjamts
authors.initials: M
authors.lastname: Enkhjargal
authors.affiliation: Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima, Tokushima 770-8502, Japan.
authors.forename: Yuanzhi
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima, Tokushima 770-8502, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Kuroda
authors.affiliation: Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima, Tokushima 770-8502, Japan.
authors.forename: Masaya
authors.initials: M
authors.lastname: Satoh
authors.affiliation: Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima, Tokushima 770-8502, Japan. Electronic address: oyama@ias.tokushima-u.ac.jp.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Oyama
mesh_terms: D000814:Aniline Compounds; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000633:toxicity*; D002118:Calcium / Q000378:metabolism*; D002224:Carbanilides / Q000633:toxicity*; D002478:Cells, Cultured; D005456:Fluorescent Dyes; D008297:Male; D051381:Rats; D060168:Thymocytes / Q000187:drug effects* / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D014966:Xanthenes; D015032:Zinc
chemical_list: D000814:Aniline Compounds; D000891:Anti-Infective Agents, Local; D002224:Carbanilides; D005456:Fluorescent Dyes; D014874:Water Pollutants, Chemical; D014966:Xanthenes; C059715:Fluo-3; C009540:triclocarban; D015032:Zinc; D002118:Calcium
keywords: Cytotoxicity; Intracellular Ca(2+); Lymphocyte; Triclocarban; Zn(2+)