title: Identification of animal rabies in Inner Mongolia and analysis of the etiologic characteristics.
abstract: OBJECTIVE
  To perform pathological observation and etiological identification of specimens collected from dairy cows, beef cattle and dogs which were suspected of rabies in Inner Mongolia in 2011, and analyze their etiological characteristics.
  
  
  METHODS
  Pathological observation was conducted on the brain specimens of three infected animals with Hematoxylin-Eosin staining, followed by confirmation using immunofluorescence and nested RT-PCR methods. Finally, phylogenetic analysis was conducted using the virus N gene sequence amplified from three specimens.
  
  
  RESULTS
  Eosinophilic and cytoplasmic inclusion bodies were seen in neuronal cells of the CNS; and rabies non-characteristic histopathological changes were also detected in the CNS. The three brain specimens were detected positive. N gene nucleotide sequence of these three isolates showed distinct sequence identity, therefore they fell into different groups in the phylogenetic analysis. N gene in the cow and dog had higher homology with that in Hebei isolate, but that in the beef cattle had higher homology with that in Mongolian lupine isolate and Russian red fox isolate.
  
  
  CONCLUSIONS
  Rabies were observed in the dairy cow, beef cattle and canine in the farm in Inner Mongolia, in 2011, which led to a different etiologic characteristics of the epidemic situation.
authors.affiliation: College of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot, 010018, Inner Mongolia, China.
authors.forename: Jing Feng
authors.initials: JF
authors.lastname: Yin
authors.affiliation: College of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot, 010018, Inner Mongolia, China.
authors.forename: Jin Ling
authors.initials: JL
authors.lastname: Wang
authors.affiliation: Key Laboratory for Medical Virology, Ministry of Health, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (IVDC, China CDC), Beijing 102206, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Tang
authors.affiliation: College of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot, 010018, Inner Mongolia, China.
authors.forename: Yu Lin
authors.initials: YL
authors.lastname: Ding
authors.affiliation: Key Laboratory for Medical Virology, Ministry of Health, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (IVDC, China CDC), Beijing 102206, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Tao
authors.affiliation: Key Laboratory for Medical Virology, Ministry of Health, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (IVDC, China CDC), Beijing 102206, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory for Medical Virology, Ministry of Health, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (IVDC, China CDC), Beijing 102206, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Song
authors.affiliation: Key Laboratory for Medical Virology, Ministry of Health, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (IVDC, China CDC), Beijing 102206, China.
authors.forename: Zhenyang
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Key Laboratory for Medical Virology, Ministry of Health, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (IVDC, China CDC), Beijing 102206, China.
authors.forename: Xin Xin
authors.initials: XX
authors.lastname: Shen
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (IVDC, China CDC), Beijing 102206, China.
authors.forename: Guo Dong
authors.initials: GD
authors.lastname: Liang
authors.affiliation: College of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot, 010018, Inner Mongolia, China.
authors.forename: Feng Long
authors.initials: FL
authors.lastname: Wang
mesh_terms: D000086:Acetazolamide; D000818:Animals; D001921:Brain / Q000473:pathology*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000473:pathology; D004283:Dog Diseases / Q000175:diagnosis* / Q000453:epidemiology; D004285:Dogs; D008986:Mongolia / Q000453:epidemiology; D009698:Nucleoproteins / Q000235:genetics; D010802:Phylogeny; D011818:Rabies / Q000453:epidemiology / Q000662:veterinary*; D011820:Rabies virus / Q000235:genetics; D013997:Time Factors
chemical_list: D009698:Nucleoproteins; D000086:Acetazolamide
keywords: Cow; Histopathology; Phylogeny; Rabies