title: Identification of soluble supramolecular FN-fibrin complexes in human plasma.
abstract: SDS-agarose FN immunoblotting of 257 normal and pathological human plasma samples revealed the ladder pattern of multiple plasma FN bands which corresponded to FN monomer and dimer, and 5 FN-fibrin bands with increasing molecular masses. The FN-fibrin bands of about 750 kDa, 1000 kDa, 1300 kDa, 1600 kDa, and 1900 kDa appeared more frequently and in significantly higher relative amounts in the pathological samples (P < 0.000) than in relatively healthy individuals. The revealing of high-molecular FN-fibrin complexes by SDS-agarose FN immunobloting might have the potential to become a laboratory biomarker of some diseases in which the coagulation system is triggered.
authors.affiliation: a Department of Chemistry and Immunochemistry , Wrocław Medical University , Wrocław , Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Krzyżanowska-Gołąb
authors.forename: Anna
authors.initials: A
authors.lastname: Lemańska-Perek
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Pupek
authors.forename: Karolina
authors.initials: K
authors.lastname: Lindner
authors.forename: Bożena
authors.initials: B
authors.lastname: Polańska
authors.forename: Irena
authors.initials: I
authors.lastname: Porębska
authors.forename: Iwona
authors.initials: I
authors.lastname: Kątnik-Prastowska
mesh_terms: D000293:Adolescent; D000328:Adult; D004587:Electrophoresis, Agar Gel; D005260:Female; D005337:Fibrin / Q000032:analysis* / Q000276:immunology; D005353:Fibronectins / Q000097:blood* / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D046911:Macromolecular Substances / Q000097:blood / Q000276:immunology; D008297:Male; D008875:Middle Aged; D012967:Sodium Dodecyl Sulfate; D012995:Solubility; D055815:Young Adult
chemical_list: D005353:Fibronectins; D046911:Macromolecular Substances; D012967:Sodium Dodecyl Sulfate; D005337:Fibrin
keywords: fibronectin; fibronectin-fibrin complexes; high-molecular fibronectin; immunoblotting