title: Pregnancy-induced hypertension is accompanied by decreased number of circulating endothelial cells and circulating endothelial progenitor cells.
abstract: Maternal endothelial dysfunction is one of the main features of pregnancy-induced hypertension (PIH). It is generally accepted that circulating endothelial cells (CECs) and endothelial progenitor cells (EPCs) reflect the state of the endothelium, its injury and/or repair possibilities. The objective of this study was to determine whether the CECs and EPCs numbers in the circulation of women with PIH reflect the presence of this pathology. Peripheral blood cells of PIH and normotensive pregnant women were labeled with specific monoclonal antibodies. For CECs evaluation, samples were labeled with anti-CD31 and anti-CD45 antibodies; for EPCs with anti-VEGFR2/KDR and anti-CD34 antibodies. Cells were quantified by flow cytometry. The levels of both CECs (CD31(+), CD45(-)) and EPCs (CD34(+), VEGFR2/KDR(+)) in the peripheral blood of women with PIH were significantly lower compared with those of control pregnant women with normal blood pressure level. Lowered accessibility of maternal CECs and EPCs may diminish general regenerative potential of the patient endothelia, contributing to PIH symptoms and to the risk of subsequent coronary and arterial disease.
authors.affiliation: Department of Gynaecology and Obstetrics, Faculty of Health Science, Wrocław Medical University, Wrocław, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Heimrath
authors.forename: Maria
authors.initials: M
authors.lastname: Paprocka
authors.forename: Andrzej
authors.initials: A
authors.lastname: Czekanski
authors.forename: Agata
authors.initials: A
authors.lastname: Ledwozyw
authors.forename: Aneta
authors.initials: A
authors.lastname: Kantor
authors.forename: Danuta
authors.initials: D
authors.lastname: Dus
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000378:metabolism; D001775:Blood Circulation; D002452:Cell Count; D002469:Cell Separation; D042783:Endothelial Cells / Q000473:pathology*; D066026:Endothelial Progenitor Cells / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D046110:Hypertension, Pregnancy-Induced / Q000473:pathology* / Q000503:physiopathology; D011247:Pregnancy; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism; D014945:Wound Healing; D055815:Young Adult
chemical_list: D015703:Antigens, CD; C000588866:KDR protein, human; D040301:Vascular Endothelial Growth Factor Receptor-2