title: Functional evaluation of hemodynamic response during neural activation using optical microangiography integrated with dual-wavelength laser speckle imaging.
abstract: Evaluation of spatiotemporal hemodynamic and metabolic responses during neural activation is crucial in studying brain function. We explore the use of a noninvasive multifunctional optical imaging system to measure these responses in a mouse brain upon electrically stimulated neural activation, with the cranium left intact. The system is developed by integrating an optical microangiography (OMAG) imaging system with a dual-wavelength laser speckle imaging (DW-LSI) system. The DW-LSI, running at an image acquisition speed of ∼100  Hz, is used to extract the large-scale two-dimensional map, revealing the localized response of blood flow, hemoglobin concentration, and metabolic rate of oxygen change. Guided by DW-LSI, the OMAG is, however, used to image the response of individual blood vessels with its unique depth-resolved capability. We show that the integrated system is capable of investigating neural activation, thus is potentially valuable in the preclinical study of the mechanism of neurovascular coupling.
authors.forename: Jia
authors.initials: J
authors.lastname: Qin
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.forename: Hequn
authors.initials: H
authors.lastname: Wang
authors.forename: Roberto
authors.initials: R
authors.lastname: Reif
authors.forename: Ruikang K
authors.initials: RK
authors.lastname: Wang
mesh_terms: D000792:Angiography / Q000379:methods*; D000818:Animals; D001921:Brain / Q000098:blood supply / Q000502:physiology; D002560:Cerebrovascular Circulation / Q000502:physiology*; D046690:Deep Brain Stimulation; D006439:Hemodynamics / Q000502:physiology*; D051379:Mice; D061848:Optical Imaging / Q000379:methods*