title: Unique features of the transcriptional response to model aneuploidy in human cells.
abstract: BACKGROUND
  Aneuploidy, a karyotype deviating from multiples of a haploid chromosome set, affects the physiology of eukaryotes. In humans, aneuploidy is linked to pathological defects such as developmental abnormalities, mental retardation or cancer, but the underlying mechanisms remain elusive. There are many different types and origins of aneuploidy, but whether there is a uniform cellular response to aneuploidy in human cells has not been addressed so far.
  
  
  RESULTS
  Here we evaluate the transcription profiles of eleven trisomic and tetrasomic cell lines and two cell lines with complex aneuploid karyotypes. We identify a characteristic aneuploidy response pattern defined by upregulation of genes linked to endoplasmic reticulum, Golgi apparatus and lysosomes, and downregulation of DNA replication, transcription as well as ribosomes. Strikingly, complex aneuploidy elicits the same transcriptional changes as trisomy. To uncover the triggers of the response, we compared the profiles with transcription changes in human cells subjected to stress conditions. Interestingly, we found an overlap only with the response to treatment with the autophagy inhibitor bafilomycin A1. Finally, we identified 23 genes whose expression is significantly altered in all aneuploids and which may thus serve as aneuploidy markers.
  
  
  CONCLUSIONS
  Our analysis shows that despite the variability in chromosome content, aneuploidy triggers uniform transcriptional response in human cells. A common response independent of the type of aneuploidy might be exploited as a novel target for cancer therapy. Moreover, the potential aneuploidy markers identified in our analysis might represent novel biomarkers to assess the malignant potential of a tumor.
authors.forename: Milena
authors.initials: M
authors.lastname: Dürrbaum
authors.forename: Anastasia Yurievna
authors.initials: AY
authors.lastname: Kuznetsova
authors.forename: Verena
authors.initials: V
authors.lastname: Passerini
authors.forename: Silvia
authors.initials: S
authors.lastname: Stingele
authors.forename: Gabriele
authors.initials: G
authors.lastname: Stoehr
authors.affiliation: Group Maintenance of Genome Stability, Max Planck Institute of Biochemistry, Am Klopferspitz 18, Martinsried 82152, Germany. storchov@biochem.mpg.de.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Storchová
mesh_terms: D000782:Aneuploidy; D005819:Genetic Markers; D045325:HCT116 Cells; D006801:Humans; D059785:Karyotype; D008957:Models, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D058670:Tetrasomy; D014314:Trisomy
chemical_list: D005819:Genetic Markers; D012333:RNA, Messenger