title: Microarray analysis revealed markedly differential miRNA expression profiles in cervical intraepithelial neoplasias and invasive squamous cell carcinoma.
abstract: OBJECTIVE
  To investigate the alterations in miRNA expression during the progression of dysplasia in cervical epithelium.
  
  
  METHODS
  A global miRNA expression profile of normal cervical squamous epithelium (Normal), cervical intraepithelial neoplasia (CIN) 3 and invasive squamous cell carcinoma (ISCC) was produced using the seventh generation of the miRCURY™ LNA microRNA Array (Exiqon, Vedbaek, Denmark). The reliability of miRNA arrays was verified by reverse transcription PCR.
  
  
  RESULTS
  Normal, CIN 3 and ISCC showed distinct miRNA expression profiles. The differentially expressed miRNAs in ISCC versus CIN 3 clearly differed from that in CIN 3 versus Normal. Compared with ISCC versus Normal, more identical miRNAs were found in ISCC versus CIN 3 than in CIN 3 versus Normal.
  
  
  CONCLUSIONS
  A particular set of miRNAs was associated with the progression of normal cervical epithelium to CIN 3 and CIN 3 to ISCC. The miRNA profile changed more noticeably in the progression of CIN to ISCC than normal cervical epithelium to CIN.
authors.affiliation: Department of Pathology, The First Affiliated Hospital, Sun Yat-sen (Zhongshan) University, Guangzhou, The People's Republic of China. yuli5@mail.sysu.edu.cn.
authors.forename: Shuang
authors.initials: S
authors.lastname: Liang
authors.forename: Tian
authors.initials: T
authors.lastname: Tian
authors.forename: Xubin
authors.initials: X
authors.lastname: Liu
authors.forename: Huijuan
authors.initials: H
authors.lastname: Shi
authors.forename: Cuilan
authors.initials: C
authors.lastname: Tang
authors.forename: Shicong
authors.initials: S
authors.lastname: Yang
authors.forename: Liantang
authors.initials: L
authors.lastname: Wang
authors.forename: Shangwu
authors.initials: S
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Yu
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D018450:Disease Progression; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D015203:Reproducibility of Results; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology; D002578:Uterine Cervical Dysplasia / Q000235:genetics* / Q000473:pathology
chemical_list: D035683:MicroRNAs
keywords: CIN; FFPE tissue; cervical cancer; cervical intraepithelial neoplasias; formalin-fixed, paraffin-embedded tissue; miRNAs; microRNAs; microarray