title: Enterococcus faecium and Enterococcus faecalis in blood of newborns with suspected nosocomial infection.
abstract: Enterococci are Gram-positive cocci saprophyte of the human gastrointestinal tract, diners who act as opportunistic pathogens. They can cause infections in patients hospitalized for a long time or who have received multiple antibiotic therapy. Enterococcus faecalis and Enterococcus faecium are the most common species in human infections. To evaluate the possibility of rapid detection of these species and their occurrence in the blood of newborns with suspected nosocomial infection, blood samples were collected from 50 newborns with late infections, admitted to the Neonatal Care Unit of the University Hospital Federal de Mato Grosso do Sul (UFMS-HU), from September 2010 to January 2011. The samples were subjected to conventional PCR and real time PCR (qPCR) to search for Enterococcus faecium and Enterococcus faecalis, respectively. The PCR results were compared with respective blood cultures from 40 patients. No blood cultures were positive for Enterococci, however, eight blood samples were identified as genomic DNA of Enterococcus faecium by qPCR and 22 blood samples were detected as genomic DNA of Enterococcus faecalis by conventional PCR. These findings are important because of the clinical severity of the evaluated patients who were found positive by conventional PCR and not through routine microbiological methods.
authors.affiliation: Program Graduate Health and Development in the Midwest Region, Federal University of Mato Grosso do Sul, Campo Grande, Brazil, Program Graduate Health and Development in the Midwest Region, Federal University of Mato Grosso do Sul, Campo Grande, Brazil.
authors.forename: Isabela
authors.initials: I
authors.lastname: Furtado
authors.affiliation: Program Graduate Health and Development in the Midwest Region, Federal University of Mato Grosso do Sul, Campo Grande, Brazil, Program Graduate Health and Development in the Midwest Region, Federal University of Mato Grosso do Sul, Campo Grande, Brazil.
authors.forename: Paula Cristhina Niz
authors.initials: PC
authors.lastname: Xavier
authors.affiliation: Department of Physical Therapy, Dom Bosco Catholic University and the Federal University of Mato Grosso do Sul, Campo Grande, Brazil, Department of Physical Therapy, Dom Bosco Catholic University and the Federal University of Mato Grosso do Sul, Campo Grande, Brazil.
authors.forename: Luciana Venhofen Martinelli
authors.initials: LV
authors.lastname: Tavares
authors.affiliation: Department of Physiology and Biofísica-ICB/UFMG, Belo Horizonte, Brazil, Department of Physiology and Biofísica-ICB/UFMG, Belo Horizonte, Brazil.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Alves
authors.affiliation: Department of Physiology and Biofísica-ICB/UFMG, Belo Horizonte, Brazil, Department of Physiology and Biofísica-ICB/UFMG, Belo Horizonte, Brazil.
authors.forename: Sarah Fonseca
authors.initials: SF
authors.lastname: Martins
authors.affiliation: Department of Physiology and Biofísica-ICB/UFMG, Belo Horizonte, Brazil, Department of Physiology and Biofísica-ICB/UFMG, Belo Horizonte, Brazil.
authors.forename: Almir de Sousa
authors.initials: Ade S
authors.lastname: Martins
authors.affiliation: Department of Pediatrics, University Hospital, Federal University of Mato Grosso do Sul, Campo Grande, Brazil, Department of Pediatrics, University Hospital, Federal University of Mato Grosso do Sul, Campo Grande, Brazil.
authors.forename: Durval Batista
authors.initials: DB
authors.lastname: Palhares
mesh_terms: D015373:Bacterial Typing Techniques; D003428:Cross Infection / Q000175:diagnosis* / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D013293:Enterococcus faecalis / Q000235:genetics* / Q000302:isolation & purification; D016984:Enterococcus faecium / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D007231:Infant, Newborn; D007363:Intensive Care Units, Neonatal; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity
chemical_list: D004269:DNA, Bacterial