title: Distinct cellular migration induced by Leishmania infantum chagasi and saliva from Lutzomyia longipalpis in a hemorrhagic pool model.
abstract: Recruitment of a specific cell population after Leishmania infection can influence the outcome of the disease. Cellular migration in response to Leishmania or vector saliva has been reported in air pouch model, however, cellular migration induced by Leishmania associated with host's blood and vector saliva in this model has not been described. Herein we investigated cellular migration into air pouch of hamster after stimulation with combination of L. chagasi and host's blood and Lutzomyia longipalpis saliva. Migration induced by saliva was 3-fold more than those induced by L. chagasi alone. Additionally, L. chagasi associated with blood and saliva induced significantly even more leukocytes into air pouch than Leishmania alone. L. chagasi recruited a diverse cell population; however, most of these cells seem to have not migrated to the inflammatory exudate, remaining in the pouch lining tissue. These results indicate that L. chagasi can reduce leukocyte accumulation to the initial site of infection, and when associated with vector saliva in the presence of blood components, increase the influx of more neutrophils than macrophages, suggesting that the parasite has developed a strategy to minimize the initial inflammatory response, allowing an unlimited progression within the host. This work reinforces the importance of studies on the salivary components of sand fly vectors of leishmaniasis in the transmission process and the establishment of the infection.
authors.affiliation: Faculdade de Medicina, Universidade Federal do Ceará, 60430-160FortalezaCE, Brazil, Faculdade de Medicina, Universidade Federal do Ceará, Rua Alexandre Baraúna 949, 60430-160 Fortaleza, CE, Brazil.
authors.forename: Camila Oliveira
authors.initials: CO
authors.lastname: Vasconcelos
authors.affiliation: Faculdade de Medicina, Universidade Federal do Ceará, 60430-160FortalezaCE, Brazil, Faculdade de Medicina, Universidade Federal do Ceará, Rua Alexandre Baraúna 949, 60430-160 Fortaleza, CE, Brazil.
authors.forename: Zirlane C Branco
authors.initials: ZC
authors.lastname: Coêlho
authors.affiliation: Faculdade de Medicina, Universidade Federal do Ceará, 60430-160FortalezaCE, Brazil, Faculdade de Medicina, Universidade Federal do Ceará, Rua Alexandre Baraúna 949, 60430-160 Fortaleza, CE, Brazil.
authors.forename: Cristina de Souza
authors.initials: Cde S
authors.lastname: Chaves
authors.affiliation: Vector Molecular Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, RockvilleMaryland, USA, Vector Molecular Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland, USA.
authors.forename: Clarissa Romero
authors.initials: CR
authors.lastname: Teixeira
authors.affiliation: Faculdade de Medicina, Universidade Federal do Ceará, 60430-160FortalezaCE, Brazil, Faculdade de Medicina, Universidade Federal do Ceará, Rua Alexandre Baraúna 949, 60430-160 Fortaleza, CE, Brazil.
authors.forename: Margarida M Lima
authors.initials: MM
authors.lastname: Pompeu
authors.affiliation: Faculdade de Medicina, Universidade Federal do Ceará, 60430-160FortalezaCE, Brazil, Faculdade de Medicina, Universidade Federal do Ceará, Rua Alexandre Baraúna 949, 60430-160 Fortaleza, CE, Brazil.
authors.forename: Maria Jania
authors.initials: MJ
authors.lastname: Teixeira
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000276:immunology*; D006224:Cricetinae; D004195:Disease Models, Animal; D005122:Exudates and Transudates / Q000276:immunology / Q000469:parasitology; D005260:Female; D006790:Host-Parasite Interactions / Q000276:immunology; D018314:Leishmania infantum / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000276:immunology / Q000473:pathology*; D008297:Male; D011576:Psychodidae / Q000276:immunology / Q000469:parasitology*; D012463:Saliva / Q000276:immunology / Q000469:parasitology*