title: Nascent proteomes in peripheral blood mononuclear cells as a novel source for biomarker discovery in human stroke.
abstract: OBJECTIVE
  The proteome of newly synthesized proteins (nascent proteome) in peripheral blood mononuclear cells (PBMCs) can be a novel source of stroke biomarkers. Changes in the PBMC nascent proteome after stroke reflect the dynamic response-in-action not detectable in the total proteome (all existing proteins) in blood. Here, we test the application of nascent proteomics as a novel approach for stroke biomarker discovery.
  
  
  METHODS
  The PBMC nascent proteome in human blood was determined by metabolic labeling of fresh PBMC cultures with azidohomoalanine (an azide-containing methionine surrogate), followed by mass spectrometry detection and quantification of azidohomoalanine-labeled proteins. The PBMC nascent and total proteomes were compared between patients with stroke and matched controls.
  
  
  RESULTS
  Both PBMC nascent and total proteomes showed differences between stroke patients and controls. Results of hierarchical clustering analysis of proteomic data revealed greater changes in the nascent than in the total PBMC proteomes, supporting the usefulness of the PBMC nascent proteome as a novel source of stroke biomarkers.
  
  
  CONCLUSIONS
  Nascent proteomes in PBMC can be a novel source for biomarker discovery in human stroke.
authors.affiliation: From the Department of Neurobiology (F.B., R.P.S., Y.L., A.Z.), and Clinical Research Center (J.W.), Morehouse School of Medicine, Atlanta, GA; Marcus Stroke and Neuroscience Center, Grady Memorial Hospital, Atlanta, GA (R.P.S., M.F.); Department of Biochemistry, Oregon Health Science University, Portland (L.D.); and Department of Neurology, Emory University, Atlanta, GA (C.L.H., M.F.).
authors.forename: Fang
authors.initials: F
authors.lastname: Bian
authors.forename: Roger P
authors.initials: RP
authors.lastname: Simon
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.forename: Larry
authors.initials: L
authors.lastname: David
authors.forename: Jolita
authors.initials: J
authors.lastname: Wainwright
authors.forename: Casey L
authors.initials: CL
authors.lastname: Hall
authors.forename: Michael
authors.initials: M
authors.lastname: Frankel
authors.forename: An
authors.initials: A
authors.lastname: Zhou
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives / Q000494:pharmacology; D015415:Biomarkers / Q000378:metabolism; D002478:Cells, Cultured; D016000:Cluster Analysis; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D010865:Pilot Projects; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods; D020521:Stroke / Q000175:diagnosis* / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D020543:Proteome; C506098:azidohomoalanine; D000409:Alanine
keywords: biomarkers; leukocytes; proteomics; stroke