title: [Research on early diagnosis of esophageal cancer by Raman spectroscopy of human hemoglobin].
abstract: The Raman spectrums of hemoglobin from 20 normal persons and 21 esophageal cancer patients were measured and analyzed in the present paper, the results show obvious differences between the Raman spectrums of esophageal cancer patients and normal persons. Compared with normal persons, there are more iron ions in low spin state and less in high spin state in the hemoglobin of esophageal cancer patients. It indicates that some iron ions in high spin state turned to low spin state because of cancerization, and this experimental result coincides with the fact that hemolysis is more likely to occur in cancer patients. Principal component analysis (PCA) was employed to get a three-dimensional scatter plot of PC scores for the health and cancer groups, and it can be learned that they are distributed in separate areas. By using the method of discriminate analysis, it was found that the diagnostic algorithm separates the two groups with sensitivity of 90.5% and diagnostic specificity of 95%, and the overall diagnostic accuracy was 92.7%. The results from this exploratory study demonstrate that Raman spectroscopy combined with multivariate analysis would be an effective method for early diagnosis of esophageal cancer.
authors.affiliation: College of Physical Science and Engineering, Zhengzhou University, Zhengzhou 450052, China. 185226112@qq.com
authors.forename: Xue
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Cancer Hospital of Zhengzhou University, Zhengzhou 450046, China.
authors.forename: Guang-Yi
authors.initials: GY
authors.lastname: Chen
authors.affiliation: College of Physical Science and Engineering, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Zhang
authors.affiliation: College of Physical Science and Engineering, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Chun-Zhen
authors.initials: CZ
authors.lastname: Fan
authors.affiliation: College of Physical Science and Engineering, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Er-Jun
authors.initials: EJ
authors.lastname: Liang
authors.affiliation: College of Physical Science and Engineering, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
authors.affiliation: College of Physical Science and Engineering, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Wei-Yue
authors.initials: WY
authors.lastname: Tang
mesh_terms: D042241:Early Diagnosis; D004938:Esophageal Neoplasms / Q000175:diagnosis*; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D015999:Multivariate Analysis; D025341:Principal Component Analysis; D013059:Spectrum Analysis, Raman
chemical_list: D006454:Hemoglobins