title: Determination of ctxAB expression in Vibrio cholerae Classical and El Tor strains using Real-Time PCR.
abstract: Cholera is an infection of the small intestines caused by the bacterium V. cholerae. It is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. The major virulence factor produced by V. cholerae during infection is the cholera toxin. Total mRNA extraction and reverse transcription was performed for making ctxAB cDNA. Relative Real-Time PCR analysis showed unequal enterotoxin production in V. cholerae strains. The results showed that, classical strain produces cholera toxin more than El Tor strain.
authors.affiliation: Cellular and Molecular Biology Research Center, (CMBRC), Babol University of Medical Sciences, Babol, Iran. ; Department of Microbiology and Immunology, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Seyed Mahmoud
authors.initials: SM
authors.lastname: Amin Marashi
authors.affiliation: Infectious Diseases & Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Ramazan
authors.initials: R
authors.lastname: Rajabnia
authors.affiliation: Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Abbas Ali
authors.initials: AA
authors.lastname: Imani Fooladi
authors.affiliation: Genetics Division, Biology Department, Faculty of Sciences, University of Isfahan, Isfahan, Iran.
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Hojati
authors.affiliation: Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Sharareh
authors.initials: S
authors.lastname: Moghim
authors.affiliation: Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Bahram
authors.initials: B
authors.lastname: Nasr Esfahani
keywords: RT-qPCR; Vibrio cholerae; ctxAB expression