title: Microbial microleakage assessment of a new hydrophilic fissure sealant: a laboratory study.
abstract: OBJECTIVE
  The purpose of this study was to compare microbial leakage of a new hydrophilic sealant with that of a conventional hydrophobic resin-based sealant.
  
  
  METHODS
  One hundred extracted, caries-free, human maxillary premolars were randomly divided into five groups. Those in Groups 1, 2, and 3 had dry, wet, and artificial saliva-contaminated occlusal surfaces, respectively, and were sealed with a hydrophilic sealant, while those in Groups 4 and 5 had dry and wet occlusal surfaces, respectively, and were sealed with a hydrophobic sealant. A newly designed microbial penetration method utilizing Streptococcus mutans as an indicator was tested for leakage assessment. Data were analyzed using SPSS 15.0 software, and the significance level was set at α=0.05.
  
  
  RESULTS
  The log rank test indicated a statistically significant difference in leakage rates among the five groups. Mantel-Cox log-rank test findings showed that Group 3 had the highest leakage rate, with Groups 2 and 4 having the lowest. There was no statistically significant difference in leakage rate between Groups 2 and 4.
  
  
  CONCLUSIONS
  With respect to the limitations of an in vitro study, our findings suggest that hydrophilic sealants are an acceptable alternative to hydrophobic sealants.
authors.affiliation: Department of Pedodontics, Dental School, at Rafsanjan University of Medical Sciences, Rafsanjan, Kerman province, Iran. drbagherian@yahoo.com.
authors.forename: Ali
authors.initials: A
authors.lastname: Bagherian
authors.affiliation: Department of Pedodontics, Dental School, at Rafsanjan University of Medical Sciences, Rafsanjan, Kerman province, Iran.
authors.forename: Mahsa
authors.initials: M
authors.lastname: Ahmadkhani
authors.affiliation: Department of Social Medicine and Environment Research Center, in the Medical School, at Rafsanjan University of Medical Sciences, Rafsanjan, Kerman province, Iran.
authors.forename: Mahmood
authors.initials: M
authors.lastname: Sheikhfathollahi
authors.affiliation: Department of Microbiology, in the Medical School, at Rafsanjan University of Medical Sciences, Rafsanjan, Kerman province, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Bahramabadinejad
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D003188:Composite Resins / Q000737:chemistry; D055117:Curing Lights, Dental; D003763:Dental Leakage / Q000145:classification / Q000382:microbiology*; D003890:Desiccation; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D055115:Light-Curing of Dental Adhesives / Q000379:methods; D008422:Materials Testing; D010756:Phosphoric Acids / Q000737:chemistry; D010895:Pit and Fissure Sealants / Q000737:chemistry*; D019279:Resin Cements / Q000737:chemistry; D012464:Saliva, Artificial / Q000737:chemistry; D013295:Streptococcus mutans / Q000502:physiology; D013997:Time Factors
chemical_list: C500418:Clinpro Sealant; D003188:Composite Resins; D010756:Phosphoric Acids; D010895:Pit and Fissure Sealants; D019279:Resin Cements; D012464:Saliva, Artificial; C030242:phosphoric acid