title: An in-vitro investigation of the effect of perfluorooctane sulphonate on cell lines of embryonic origin.
abstract: Fluorinated organic compounds, such as perfluorooctane sulfonate, are stable chemicals with a wide range of industrial applications. The potential toxicity of perfluorooctane sulfonate is not well characterized, and even less known are the mechanisms underlying its toxic effects. Perfluorooctane sulfonate change of inner mitochondrial membrane permeability has been implicated as a potential mechanism of toxicity. In this study, we research that perfluorooctane sulfonate effects the expression of Apaf1 and Caspase3 genes in the amnion and fetal lung cell line that initiate the cells to undergo apoptosis. The expression of Caspase3 and Apaf1 was determined by using quantitative RT-PCR. In the study there is significant increase in expression of Caspase3 and Apaf1 in amnion and fetal lung cell line exposed to high dose (p < 0.001, p = 0.004). Also there is significant increase in cell lines exposed for a long period of time to perfluorooctane sulfonate (p = 0.001). But no significant increase was seen in the low doses and exposed for a short period of time. In conclusion, apoptotic gene expression is increase in cells exposed perfluorooctane sulfonate by dose dependent manner was determined. So this work is the first study examines the apoptotic effects of perfluorooctane sulfonate in human embryonic cells it will lead the way to the other topical studies.
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Bulent Ecevit University, Zonguldak, Turkey, sevimkarakas@yahoo.com.
authors.forename: Sevim
authors.initials: S
authors.lastname: Karakas-Celik
authors.forename: Nurcan
authors.initials: N
authors.lastname: Aras
mesh_terms: D017738:Alkanesulfonic Acids / Q000008:administration & dosage* / Q000633:toxicity; D017209:Apoptosis / Q000187:drug effects*; D053477:Apoptotic Protease-Activating Factor 1 / Q000096:biosynthesis; D053148:Caspase 3 / Q000096:biosynthesis; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000187:drug effects; D053595:Embryonic Stem Cells / Q000187:drug effects; D004785:Environmental Pollutants / Q000008:administration & dosage / Q000633:toxicity; D005466:Fluorocarbons / Q000008:administration & dosage* / Q000633:toxicity; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D051336:Mitochondrial Membranes / Q000187:drug effects
chemical_list: C506592:APAF1 protein, human; D017738:Alkanesulfonic Acids; D053477:Apoptotic Protease-Activating Factor 1; D004785:Environmental Pollutants; D005466:Fluorocarbons; C076994:perfluorooctane sulfonic acid; D053148:Caspase 3