title: Buffering the pH of the culture medium does not extend yeast replicative lifespan.
abstract: During chronological aging of budding yeast cells, the culture medium can become acidified, and this acidification limits cell survival.  As a consequence, buffering the culture medium to pH 6 significantly extends chronological life span under standard conditions in synthetic medium.  In this study, we assessed whether a similar process occurs during replicative aging of yeast cells.  We find no evidence that buffering the pH of the culture medium to pH levels either higher or lower than the initial pH of the medium is able to significantly extend replicative lifespan.  Thus, we conclude that, unlike chronological life span, replicative life span is not limited by acidification of the culture medium or by changes in the pH of the environment.
authors.affiliation: Department of Pathology, University of Washington, Seattle, WA, 98195, USA.
authors.forename: Brian M
authors.initials: BM
authors.lastname: Wasko
authors.affiliation: Department of Pathology, University of Washington, Seattle, WA, 98195, USA.
authors.forename: Daniel T
authors.initials: DT
authors.lastname: Carr
authors.affiliation: Department of Pathology, University of Washington, Seattle, WA, 98195, USA.
authors.forename: Herman
authors.initials: H
authors.lastname: Tung
authors.affiliation: Department of Pathology, University of Washington, Seattle, WA, 98195, USA.
authors.forename: Ha
authors.initials: H
authors.lastname: Doan
authors.affiliation: Department of Pathology, University of Washington, Seattle, WA, 98195, USA.
authors.forename: Nathan
authors.initials: N
authors.lastname: Schurman
authors.affiliation: Department of Pathology, University of Washington, Seattle, WA, 98195, USA.
authors.forename: Jillian R
authors.initials: JR
authors.lastname: Neault
authors.affiliation: Department of Pathology, University of Washington, Seattle, WA, 98195, USA.
authors.forename: Joey
authors.initials: J
authors.lastname: Feng
authors.affiliation: Department of Pathology, University of Washington, Seattle, WA, 98195, USA.
authors.forename: Janet
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Pathology, University of Washington, Seattle, WA, 98195, USA.
authors.forename: Ben
authors.initials: B
authors.lastname: Zipkin
authors.affiliation: Department of Pathology, University of Washington, Seattle, WA, 98195, USA.
authors.forename: Jacob
authors.initials: J
authors.lastname: Mouser
authors.affiliation: Department of Pathology, University of Washington, Seattle, WA, 98195, USA.
authors.forename: Edward
authors.initials: E
authors.lastname: Oudanonh
authors.affiliation: Department of Pathology, University of Washington, Seattle, WA, 98195, USA.
authors.forename: Tina
authors.initials: T
authors.lastname: Nguyen
authors.affiliation: Department of Pathology, University of Washington, Seattle, WA, 98195, USA.
authors.forename: Torin
authors.initials: T
authors.lastname: Stetina
authors.affiliation: Department of Pathology, University of Washington, Seattle, WA, 98195, USA.
authors.forename: Anna
authors.initials: A
authors.lastname: Shemorry
authors.affiliation: Department of Pathology, University of Washington, Seattle, WA, 98195, USA.
authors.forename: Mekedes
authors.initials: M
authors.lastname: Lemma
authors.affiliation: Department of Pathology, University of Washington, Seattle, WA, 98195, USA.
authors.forename: Matt
authors.initials: M
authors.lastname: Kaeberlein