title: Assessing the intracellular concentration of platinum in medulloblastoma cell lines after Cisplatin incubation.
abstract: Two different analytical approaches, external calibration and isotope dilution analysis both using flow-injection inductively coupled plasma mass spectrometry, have been developed and applied to determine the intracellular platinum concentration after Cisplatin incubation of two different medulloblastoma cell lines (UW228 and DAOY). As the internal or isotopically enriched standard was already used for cell lysis, maximum accuracy of the results was obtained, whereas a new home-built and inert injection system dramatically lowered carry-over effects and analyte loss. With limits of the detection well below 0.4μgL(-1) and typical relative standard deviations of 2%, a strong correlation between the cell viability in MTT assays and the incorporated amount of Pt could be shown, which was subsequently normalized to the protein content of the samples. DAOY cells did significantly ingest more Pt and showed a higher mortality, which supports the fact that transporter expression needs to be taken into account in order to obtain meaningful results.
authors.affiliation: University of Münster, Institute of Inorganic and Analytical Chemistry, Corrensstr. 28/30, 48149 Münster, Germany.
authors.forename: Christoph A
authors.initials: CA
authors.lastname: Wehe
authors.affiliation: University of Münster, University Hospital, Medical Clinic D, Experimental Nephrology and Interdisciplinary Center for Clinical Research (IZKF), Albert-Schweitzer-Campus 1 - A14, 48149 Münster, Germany.
authors.forename: Georg
authors.initials: G
authors.lastname: Beyer
authors.affiliation: University of Münster, Institute of Inorganic and Analytical Chemistry, Corrensstr. 28/30, 48149 Münster, Germany; European Virtual Institute for Speciation Analysis (EVISA), Mendelstr. 11, 48149 Münster, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Sperling
authors.affiliation: University of Münster, University Hospital, Medical Clinic D, Experimental Nephrology and Interdisciplinary Center for Clinical Research (IZKF), Albert-Schweitzer-Campus 1 - A14, 48149 Münster, Germany.
authors.forename: Giuliano
authors.initials: G
authors.lastname: Ciarimboli
authors.affiliation: University of Münster, Institute of Inorganic and Analytical Chemistry, Corrensstr. 28/30, 48149 Münster, Germany. Electronic address: uk@uni-muenster.de.
authors.forename: Uwe
authors.initials: U
authors.lastname: Karst
mesh_terms: D002138:Calibration; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology*; D017022:Flow Injection Analysis / Q000379:methods*; D006801:Humans; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism*; D008527:Medulloblastoma / Q000378:metabolism* / Q000473:pathology; D010984:Platinum / Q000378:metabolism*; D013054:Spectrophotometry, Atomic / Q000379:methods*
chemical_list: D010984:Platinum; D002945:Cisplatin
keywords: Cisplatin; Cytostatics; Flow-injection; ICP-MS; Intracellular quantitation