title: Synergistic exposure of rice seeds to different doses of γ-ray and salinity stress resulted in increased antioxidant enzyme activities and gene-specific modulation of TC-NER pathway.
abstract: Recent reports have underlined the potential of gamma (γ)-rays as tools for seed priming, a process used in seed industry to increase seed vigor and to enhance plant tolerance to biotic/abiotic stresses. However, the impact of γ -rays on key aspects of plant metabolism still needs to be carefully evaluated. In the present study, rice seeds were challenged with different doses of γ -rays and grown in absence/presence of NaCl to assess the impact of these treatments on the early stages of plant life. Enhanced germination efficiency associated with increase in radicle and hypocotyl length was observed, while at later stages no increase in plant tolerance to salinity stress was evident. APX, CAT, and GR were enhanced at transcriptional level and in terms of enzyme activity, indicating the activation of antioxidant defence. The profiles of DNA damage accumulation were obtained using SCGE and the implication of TC-NER pathway in DNA damage sensing and repair mechanisms is discussed. OsXPB2, OsXPD, OsTFIIS, and OsTFIIS-like genes showed differential modulation in seedlings and plantlets in response to γ -irradiation and salinity stress. Altogether, the synergistic exposure to γ -rays and NaCl resulted in enhanced oxidative stress and proper activation of antioxidant mechanisms, thus being compatible with plant survival.
authors.affiliation: Plant Molecular Biology Group, International Center for Genetic Engineering and Biotechnology (ICGEB), New Delhi 110067, India ; Department of Biology and Biotechnology "L. Spallanzani", University of Pavia, 27100 Pavia, Italy ; Plant Breeding, Genetics and Biotechnology Division, International Rice Research Institute (IRRI), 4031 Los Banos, Philippines.
authors.forename: Anca
authors.initials: A
authors.lastname: Macovei
authors.affiliation: Plant Molecular Biology Group, International Center for Genetic Engineering and Biotechnology (ICGEB), New Delhi 110067, India.
authors.forename: Bharti
authors.initials: B
authors.lastname: Garg
authors.affiliation: Plant Molecular Biology Group, International Center for Genetic Engineering and Biotechnology (ICGEB), New Delhi 110067, India.
authors.forename: Shailendra
authors.initials: S
authors.lastname: Raikwar
authors.affiliation: Department of Biology and Biotechnology "L. Spallanzani", University of Pavia, 27100 Pavia, Italy.
authors.forename: Alma
authors.identifier: 0000-0003-2003-4120
authors.initials: A
authors.lastname: Balestrazzi
authors.affiliation: Department of Biology and Biotechnology "L. Spallanzani", University of Pavia, 27100 Pavia, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Carbonera
authors.affiliation: Department of Chemistry, University of Pavia, 27100 Pavia, Italy.
authors.forename: Armando
authors.identifier: 0000-0003-0785-0801
authors.initials: A
authors.lastname: Buttafava
authors.affiliation: Plant Biotechnology Lab, Division of Molecular Biology, Instituto Potosino de Investigación Científfica y Tecnológica (IPICYT), 78216 San Luis Potosí, SLP, Mexico.
authors.forename: Juan Francisco Jiménez
authors.initials: JF
authors.lastname: Bremont
authors.affiliation: Stress Physiology and Molecular Biology Lab, Centre for Biotechnology, MD University, Rohtak 124001, India.
authors.forename: Sarvajeet Singh
authors.initials: SS
authors.lastname: Gill
authors.affiliation: Plant Molecular Biology Group, International Center for Genetic Engineering and Biotechnology (ICGEB), New Delhi 110067, India.
authors.forename: Narendra
authors.initials: N
authors.lastname: Tuteja
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects / Q000528:radiation effects; D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects; D015870:Gene Expression / Q000187:drug effects / Q000528:radiation effects; D012275:Oryza / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D012639:Seeds / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D015398:Signal Transduction / Q000187:drug effects / Q000528:radiation effects; D012965:Sodium Chloride / Q000494:pharmacology*; D014867:Water / Q000032:analysis / Q000378:metabolism; D014965:X-Rays
chemical_list: D000975:Antioxidants; D014867:Water; D012965:Sodium Chloride