title: Acetylcholine receptor subunit and P-glycoprotein transcription patterns in levamisole-susceptible and -resistant Haemonchus contortus.
abstract: The mechanism of resistance to the anthelmintic levamisole in parasitic nematodes is poorly understood, although there is some evidence implicating changes in expression of nicotinic acetylcholine receptor (nAChR) subunit genes. Hence, in order to define levamisole resistance mechanisms in some Australian field-derived isolates of Haemonchus contortus we examined gene expression patterns and SNPs in nAChR subunit genes, as well as expression levels for P-glycoprotein (P-gp) and receptor ancillary protein genes, in various life stages of one levamisole-sensitive and three levamisole-resistant isolates of this species. Larvae of two isolates showed high-level resistance to levamisole (resistance ratios at the IC50 > 600) while the third isolate showed a degree of heterogeneity, with a resistance factor of only 1.1-fold at the IC50 alongside the presence of a resistant subpopulation. Transcription patterns for nAChR subunit genes showed a great degree of variability across the different life stages and isolates. The most consistent observation was the down-regulation of Hco-unc-63a in adults of all resistant isolates. Transcription of this gene was also reduced in the L3 stage of the two most resistant isolates, highlighting its potential as a resistance marker in the readily accessible free-living stages. There was down regulation of all four Hco-unc-29 paralogs in adults of one resistant isolate. There were no consistent changes in expression of P-gps or ancillary protein genes across the resistant isolates. The present study has demonstrated a complex pattern of nAChR subunit gene expression in H. contortus, and has highlighted several instances where reduced expression of subunit genes (Hco-unc-63a, Hco-unc-29) may be associated with the observed levamisole resistance. The data also suggests that it will be difficult to detect resistance using gene transcription-based methods on pooled larval samples from isolates containing only a resistant subpopulation due to the averaging of gene expression data across the whole population.
authors.affiliation: CSIRO Animal, Food and Health Sciences, 306 Carmody Rd., St. Lucia, Brisbane QLD 4067, Australia ; School of Veterinary Science, University of Queensland, Gatton QLD 4341, Australia.
authors.forename: Ranbir S
authors.initials: RS
authors.lastname: Sarai
authors.affiliation: School of Veterinary Science, University of Queensland, Gatton QLD 4341, Australia.
authors.forename: Steven R
authors.initials: SR
authors.lastname: Kopp
authors.affiliation: School of Veterinary Science, University of Queensland, Gatton QLD 4341, Australia.
authors.forename: Glen T
authors.initials: GT
authors.lastname: Coleman
authors.affiliation: CSIRO Animal, Food and Health Sciences, 306 Carmody Rd., St. Lucia, Brisbane QLD 4067, Australia.
authors.forename: Andrew C
authors.initials: AC
authors.lastname: Kotze
keywords: Haemonchus contortus; Levamisole; Nicotinic agonists; Resistance