title: Kinetic analysis of in vitro pre-mRNA splicing in HeLa nuclear extract.
abstract: Kinetic analysis of in vitro splicing is a valuable technique for understanding splicing regulation. It allows the determination of specific contributions from functional elements for the efficient removal of introns. This chapter will describe the rationale and approach employed to use kinetic analysis to evaluate an in vitro splicing reaction using radiolabeled pre-mRNA incubated in splicing-competent HeLa nuclear extract (NE).
authors.affiliation: Department of Microbiology & Molecular Genetics, University of California, Irvine, CA, USA.
authors.forename: William F
authors.initials: WF
authors.lastname: Mueller
authors.forename: Klemens J
authors.initials: KJ
authors.lastname: Hertel
mesh_terms: D017398:Alternative Splicing / Q000235:genetics*; D002457:Cell Extracts / Q000235:genetics* / Q000302:isolation & purification; D002467:Cell Nucleus / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008967:Molecular Biology / Q000379:methods*; D012322:RNA Precursors / Q000235:genetics
chemical_list: D002457:Cell Extracts; D012322:RNA Precursors