title: Porous platinum nanotubes labeled with hemin/G-quadruplex based electrochemical aptasensor for sensitive thrombin analysis via the cascade signal amplification.
abstract: For the first time, a sensitive electrochemical aptasensor for thrombin (TB) was developed by using porous platinum nanotubes (PtNTs) labeled with hemin/G-quadruplex and glucose dehydrogenase (GDH) as labels. Porous PtNTs with large surface area exhibited the peroxidase-like activity. Coupling with GDH and hemin/G-quadruplex as NADH oxidase and HRP-mimicking DNAzyme, the cascade signal amplification was achieved by the following ways: in the presence of glucose and NAD(+) in the working buffer, GDH electrocatalyzed the oxidation of glucose with the production of NADH. Then, hemin/G-quadruplex as NADH oxidase catalyzed the oxidation of NADH to in situ generate H2O2. Based on the corporate electrocatalysis of PtNTs and hemin/G-quadruplex toward H2O2, the electrochemical signal was significantly amplified, allowing the detection limit of TB down to 0.15 pM level. Moreover, the proposed strategy was simple because the intercalated hemin offered the readout signal, avoiding the adding of additional redox mediator as signal donator. Such an electrochemical aptasensor is highly promising for sensitive detection of other proteins in clinical diagnostics.
authors.affiliation: Department of Chemistry and Chemical Engineering, Xinxiang University, Xinxiang 453000, PR China. Electronic address: ailisun77@gmail.com.
authors.forename: Aili
authors.initials: A
authors.lastname: Sun
authors.affiliation: Southwest Hospital & Institute of Hepatobilitary Surgery, the Third Military Medical University, Chongqing 400038, PR China; Department of General Surgery, the 371th Hospital of PLA, Xinxiang 453000, PR China.
authors.forename: Qingan
authors.initials: Q
authors.lastname: Qi
authors.affiliation: Department of Chemistry and Chemical Engineering, Xinxiang University, Xinxiang 453000, PR China.
authors.forename: Xuannian
authors.initials: X
authors.lastname: Wang
authors.affiliation: Southwest Hospital & Institute of Hepatobilitary Surgery, the Third Military Medical University, Chongqing 400038, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Bie
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods; D021881:DNA, Catalytic / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods; D054856:G-Quadruplexes; D006427:Hemin / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D057230:Limit of Detection; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D010984:Platinum / Q000737:chemistry*; D016062:Porosity; D013917:Thrombin / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D021881:DNA, Catalytic; C081676:thrombin aptamer; D010984:Platinum; D006427:Hemin; D006861:Hydrogen Peroxide; D013917:Thrombin
keywords: Cascade signal amplification; Electrochemical aptasensor; Glucose dehydrogenase; Hemin/G-quadruplex; Porous PtNTs