title: Administration of reconstituted polyphenol oil bodies efficiently suppresses dendritic cell inflammatory pathways and acute intestinal inflammation.
abstract: Polyphenols are natural compounds capable of interfering with the inflammatory pathways of several in vitro model systems. In this study, we developed a stable and effective strategy to administer polyphenols to treat in vivo models of acute intestinal inflammation. The in vitro suppressive properties of several polyphenols were first tested and compared for dendritic cells (DCs) production of inflammatory cytokines. A combination of the polyphenols, quercetin and piperine, were then encapsulated into reconstituted oil bodies (OBs) in order to increase their stability. Our results showed that administration of low dose reconstituted polyphenol OBs inhibited LPS-mediated inflammatory cytokine secretion, including IL-6, IL-23, and IL-12, while increasing IL-10 and IL-1Rα production. Mice treated with the polyphenol-containing reconstituted OBs (ROBs) were partially protected from dextran sodium sulfate (DSS)-induced colitis and associated weight loss, while mortality and inflammatory scores revealed an overall anti-inflammatory effect that was likely mediated by impaired DC immune responses. Our study indicates that the administration of reconstituted quercetin and piperine-containing OBs may represent an effective and potent anti-inflammatory strategy to treat acute intestinal inflammation.
authors.affiliation: Laboratory of Experimental Immunopathology, IRCCS "de Bellis," Castellana Grotte (BA), Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Cavalcanti
authors.affiliation: Laboratory of Experimental Immunopathology, IRCCS "de Bellis," Castellana Grotte (BA), Italy ; Laboratory of Mucosal Immunology, Mario Negri South, Santa Maria Imbaro (CH), Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Vadrucci
authors.affiliation: Laboratory of Mucosal Immunology, Mario Negri South, Santa Maria Imbaro (CH), Italy ; Laboratory of Translational Medicine, ARCHES, Castellana Grotte (BA), Italy.
authors.forename: Francesca Romana
authors.initials: FR
authors.lastname: Delvecchio
authors.affiliation: Laboratory of Experimental Immunopathology, IRCCS "de Bellis," Castellana Grotte (BA), Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Addabbo
authors.affiliation: Department of Engineering for Innovation, University of Salento, Lecce, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Bettini
authors.affiliation: Department of Pediatrics, Case Western Reserve University School of Medicine, Cleveland, Ohio, United States of America.
authors.forename: Rachel
authors.initials: R
authors.lastname: Liou
authors.affiliation: Department of Pathology and Diagnostic, University of Verona Medical School, Verona, Italy.
authors.forename: Vladia
authors.initials: V
authors.lastname: Monsurrò
authors.affiliation: Department of Pediatrics, Case Western Reserve University School of Medicine, Cleveland, Ohio, United States of America.
authors.forename: Alex Yee-Chen
authors.initials: AY
authors.lastname: Huang
authors.affiliation: Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, Ohio, United States of America.
authors.forename: Theresa Torres
authors.initials: TT
authors.lastname: Pizarro
authors.affiliation: Institute of Science of Food Production, CNR, Lecce, Italy.
authors.forename: Angelo
authors.initials: A
authors.lastname: Santino
authors.affiliation: Laboratory of Experimental Immunopathology, IRCCS "de Bellis," Castellana Grotte (BA), Italy ; Laboratory of Mucosal Immunology, Mario Negri South, Santa Maria Imbaro (CH), Italy ; Laboratory of Translational Medicine, ARCHES, Castellana Grotte (BA), Italy.
authors.forename: Marcello
authors.initials: M
authors.lastname: Chieppa
mesh_terms: D000208:Acute Disease; D000470:Alkaloids / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D052117:Benzodioxoles / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D002214:Capsules; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy / Q000276:immunology / Q000378:metabolism; D003713:Dendritic Cells / Q000187:drug effects* / Q000378:metabolism; D016264:Dextran Sulfate / Q000009:adverse effects; D004305:Dose-Response Relationship, Drug; D004355:Drug Stability; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy / Q000276:immunology / Q000378:metabolism; D015850:Interleukin-6 / Q000096:biosynthesis; D007410:Intestinal Diseases / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008081:Liposomes; D051379:Mice; D010457:Peptidoglycan / Q000494:pharmacology; D010880:Piperidines / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D059808:Polyphenols / Q000008:administration & dosage* / Q000494:pharmacology* / Q000627:therapeutic use; D053284:Polyunsaturated Alkamides / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D011794:Quercetin / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D000470:Alkaloids; D052117:Benzodioxoles; D002214:Capsules; D015850:Interleukin-6; D008070:Lipopolysaccharides; D008081:Liposomes; D010457:Peptidoglycan; D010880:Piperidines; D059808:Polyphenols; D053284:Polyunsaturated Alkamides; D014409:Tumor Necrosis Factor-alpha; D016264:Dextran Sulfate; D011794:Quercetin; C008922:piperine