title: Synthesis, characterisation and evaluation of N-mannich bases of 2-substituted Benzimidazole derivatives.
abstract: BACKGROUND
  Benzimidazoles and its derivatives represent one of the mainly biological active classes of literature.
  
  
  OBJECTIVE
  In this present study aimed to synthesize N-mannich bases derivatives compounds bearing of 2-substituted benzimidazole moiety, in order to investigate their possible biological activity.
  
  
  METHODS
  Benzimidazole compounds were prepared from the condensation reaction between ortho phenylene diamine and various acids. Mannich base of newly synthesized Benzimidazole derivatives were synthesized from 2-substituted Benzimidazoles by reacting with secondary amines. The purity of the compounds was ascertained by melting point (m.p) and thin layer chromatography (TLC). Structures of the synthesized compounds were elucidated by spectral data. Antimicrobial assay was performed by microbroth dilution method. Bacterial genomic DNA cleavage was assessed by Agarose gel electrophoresis. Toxicity of the most effective compounds was studied by Brine-shrimp lethality assay.
  
  
  RESULTS
  Among the synthesized compounds, compound 5E (a) and (b) was establish to be the most potent against all tested microorganisms. This two compounds exhibited complete bacterial DNA cleavage and non-toxic.
  
  
  CONCLUSIONS
  These results suggest that it an interesting compound compared to the current therapeutic agents and are considered to investigate further for the same.
authors.affiliation: Department of Pharmaceutical Chemistry, Gokulakrishna College of Pharmacy, Andhra Pradesh 524121, India.
authors.forename: Sekar
authors.initials: S
authors.lastname: Vinoth Kumar
authors.affiliation: Department of Pharmacology, SSM College of Pharmacy, Tamil Nadu 638312, India.
authors.forename: Mohan Raj
authors.initials: MR
authors.lastname: Subramanian
authors.affiliation: Department of Pharmaceutical Biotechnology, Srikrupa Institute of Pharmaceutical Sciences, Andhra Pradesh 502277, India.
authors.forename: Santhosh Kumar
authors.initials: SK
authors.lastname: Chinnaiyan
keywords: Benzimidazoles; Brine-shrimp lethality; Mannich base; Microbroth dilution; O-Phenylene diamine