title: Marsdenia tenacissima extract sensitizes MG63 cells to doxorubicin-induced apoptosis.
abstract: Marsdenia tenacissima extract (MTE) is a new plate-derived biotechnology product that is frequently used, but occasionally reported, in the field of chemotherapy. In this study, we assessed the antitumor activity and related mechanisms of MTE by various biotechnological methods. The survival rates of MG63 osteosarcoma cells treated with MTE and doxorubicin were measured, individually or jointly, and the changes in cellular shape, apoptotic rates, and Fas expression were observed. The results indicated that combination of MTE and doxorubicin up-regulated Fas expression and induced apoptosis. The survival rate of combined application of 50 mg/mL MTE and 1 μg/mL doxorubicin was significantly lower than that of the individual application (P < 0.01). Other biotechnology methods also showed an apoptosis-inducing effect of combined application that was much stronger than individual application. All of these results suggested that MTE may promote the effects of doxorubicin chemotherapy, perhaps related to the up-regulation of Fas expression in tumor cells.
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, China huangtao@mail.cmu.edu.cn.
authors.forename: T
authors.initials: T
authors.lastname: Huang
authors.affiliation: Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
authors.forename: W H
authors.initials: WH
authors.lastname: Gong
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, China.
authors.forename: C P
authors.initials: CP
authors.lastname: Zou
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, China.
authors.forename: X C
authors.initials: XC
authors.lastname: Li
authors.affiliation: Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
authors.forename: G J
authors.initials: GJ
authors.lastname: Jiang
authors.affiliation: Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
authors.forename: X H
authors.initials: XH
authors.lastname: Li
authors.affiliation: Experimental Center of Medicine, Lanzhou General Hospital, Lanzhou Military Area Command, Key Laboratory of Stem Cells and Gene Drugs of Gansu Province, Lanzhou, Gansu, China.
authors.forename: H
authors.initials: H
authors.lastname: Qian
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology*; D006801:Humans; D031101:Marsdenia / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000903:Antibiotics, Antineoplastic; D010936:Plant Extracts; D004317:Doxorubicin