title: Multivariate Analysis Using High Definition Flow Cytometry Reveals Distinct T Cell Repertoires between the Fetal-Maternal Interface and the Peripheral Blood.
abstract: The human T cell compartment is a complex system and while some information is known on repertoire composition and dynamics in the peripheral blood, little is known about repertoire composition at different anatomical sites. Here, we determine the T cell receptor beta variable (TRBV) repertoire at the decidua and compare it with the peripheral blood during normal pregnancy and pre-eclampsia. We found total T cell subset disparity of up to 58% between sites, including large signature TRBV expansions unique to the fetal-maternal interface. Defining the functional nature and specificity of compartment-specific T cells will be necessary if we are to understand localized immunity, tolerance, and pathogenesis.
authors.affiliation: Human Immunity Laboratory and Cellular Immunology Laboratory, QIMR Berghofer Medical Research Institute , Brisbane, QLD , Australia.
authors.forename: Michelle A
authors.initials: MA
authors.lastname: Neller
authors.affiliation: Nepean Centre for Perinatal Care, Sydney Medical School, The University of Sydney , Sydney, NSW , Australia.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Santner-Nanan
authors.affiliation: Human Immunity Laboratory and Cellular Immunology Laboratory, QIMR Berghofer Medical Research Institute , Brisbane, QLD , Australia.
authors.forename: Rebekah M
authors.initials: RM
authors.lastname: Brennan
authors.affiliation: Nepean Centre for Perinatal Care, Sydney Medical School, The University of Sydney , Sydney, NSW , Australia.
authors.forename: Peter
authors.initials: P
authors.lastname: Hsu
authors.affiliation: Nepean Centre for Perinatal Care, Sydney Medical School, The University of Sydney , Sydney, NSW , Australia.
authors.forename: Steven
authors.initials: S
authors.lastname: Joung
authors.affiliation: Nepean Centre for Perinatal Care, Sydney Medical School, The University of Sydney , Sydney, NSW , Australia.
authors.forename: Ralph
authors.initials: R
authors.lastname: Nanan
authors.affiliation: Human Immunity Laboratory and Cellular Immunology Laboratory, QIMR Berghofer Medical Research Institute , Brisbane, QLD , Australia ; School of Medicine, The University of Queensland , Brisbane, QLD , Australia.
authors.forename: Scott R
authors.initials: SR
authors.lastname: Burrows
authors.affiliation: Human Immunity Laboratory and Cellular Immunology Laboratory, QIMR Berghofer Medical Research Institute , Brisbane, QLD , Australia ; School of Medicine, The University of Queensland , Brisbane, QLD , Australia ; Institute of Infection and Immunity, Cardiff University School of Medicine , Cardiff , UK.
authors.forename: John J
authors.initials: JJ
authors.lastname: Miles
keywords: T cell repertoire; decidual mononuclear cells; pre-eclampsia; pregnancy