title: Short communication: interaction of bovine milk protein with chlorpyrifos.
abstract: Dairy products are considered as nutrient-dense foods and consumed by many people in western countries, as well as an increasing number of Asian people. Excessive and frequent application of pesticides on vegetables and fruits leads to a potential health hazard to consumers. The organophosphate insecticide chlorpyrifos has been reported to bind with human and bovine serum albumin. Thus, it is necessary to explore the interaction between food protein and chlorpyrifos. In this study, equilibrium dialysis and fluorescence spectra were used to demonstrate binding of milk proteins to chlorpyrifos. The amount of milk protein bound was 0.03±0.01mg/g. Moreover, the milk protein-chlorpyrifos complexes were stable at pH 3.5to 9.5 and ion concentrations from 0.1 to 1.0M. The amount of chlorpyrifos bound to milk proteins decreased to 50% after being in vitro digested by pepsin and trypsin. The results showed that the interaction between food proteins and the pesticide might partially remove the insecticide and reduce the concentration of pesticide absorbed into the blood and, thus, alleviate the corresponding toxicity.
authors.affiliation: Beijing Laboratory of Food Quality and Safety, Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue, College of Food Science and Engineering, Beijing University of Agriculture, Beijing, China 102206.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lv
authors.affiliation: Beijing Laboratory of Food Quality and Safety, Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue, College of Food Science and Engineering, Beijing University of Agriculture, Beijing, China 102206.
authors.forename: Xuefen
authors.initials: X
authors.lastname: Li
authors.affiliation: Beijing Laboratory of Food Quality and Safety, Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue, College of Food Science and Engineering, Beijing University of Agriculture, Beijing, China 102206.
authors.forename: Zongyi
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Beijing Laboratory of Food Quality and Safety, Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue, College of Food Science and Engineering, Beijing University of Agriculture, Beijing, China 102206.
authors.forename: Han
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Beijing Laboratory of Food Quality and Safety, Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue, College of Food Science and Engineering, Beijing University of Agriculture, Beijing, China 102206.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Beijing Laboratory of Food Quality and Safety, Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue, College of Food Science and Engineering, Beijing University of Agriculture, Beijing, China 102206.
authors.forename: Ran
authors.initials: R
authors.lastname: Huo
authors.affiliation: Beijing Laboratory of Food Quality and Safety, Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue, College of Food Science and Engineering, Beijing University of Agriculture, Beijing, China 102206.
authors.forename: Xiangning
authors.initials: X
authors.lastname: Chen
authors.affiliation: Beijing Laboratory of Food Quality and Safety, Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue, College of Food Science and Engineering, Beijing University of Agriculture, Beijing, China 102206. Electronic address: taohan00@163.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Han
mesh_terms: D000818:Animals; D002417:Cattle; D004390:Chlorpyrifos / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007306:Insecticides; D008894:Milk Proteins / Q000737:chemistry*; D010434:Pepsin A / Q000737:chemistry; D014357:Trypsin / Q000737:chemistry
chemical_list: D007306:Insecticides; D008894:Milk Proteins; D014357:Trypsin; D010434:Pepsin A; D004390:Chlorpyrifos
keywords: binding; chlorpyrifos; skimmed milk; stability