title: Adaptive and innate immune molecules in developing rainbow trout, Oncorhynchus mykiss eggs and larvae: expression of genes and occurrence of effector molecules.
abstract: The ontogenetic development of the immune system was studied during the egg phase and the early post-hatch period of rainbow trout. Quantitative real-time PCR (qPCR) was used to assess the timing and degree of expression of 9 important immune relevant genes and EF1-α. Further, immunohistochemical staining using monoclonal antibodies was applied on rainbow trout embryos and larvae in order to localize five different protein molecules (MHCII, CD8, IgM, IgT and SAA) in the developing tissue and immune organs. Maternally transferred transcripts of EF1-α mRNA were detected in the unfertilized egg. Early onset of expression was seen for all immune genes at very low levels. The amount of mRNA slowly increased and peaked around and after hatching. The highest increases were seen for MHCII, C3, C5 and SAA. Immunohistochemistry using five monoclonal antibodies showed positive staining from day 84 post fertilization. Skin, gills, intestine, pseudobranch and thymus showed reactivity for MHCII, thymus for CD8, gill mucus for IgT and pseudobranch and cartilage associated tissue for SAA. The importance of detected factors for early protection of eggs and larvae is discussed.
authors.affiliation: Laboratory of Aquatic Pathobiology, Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Stigbøjlen 7, DK-1870 Frederiksberg C, Denmark. Electronic address: rdh@sund.ku.dk.
authors.forename: Rasmus D
authors.initials: RD
authors.lastname: Heinecke
authors.affiliation: Laboratory of Aquatic Pathobiology, Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Stigbøjlen 7, DK-1870 Frederiksberg C, Denmark. Electronic address: jkc@sund.ku.dk.
authors.forename: Jiwan K
authors.initials: JK
authors.lastname: Chettri
authors.affiliation: Laboratory of Aquatic Pathobiology, Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Stigbøjlen 7, DK-1870 Frederiksberg C, Denmark. Electronic address: kub@sund.ku.dk.
authors.forename: Kurt
authors.initials: K
authors.lastname: Buchmann
mesh_terms: D056704:Adaptive Immunity; D000818:Animals; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000276:immunology*; D007113:Immunity, Innate; D007814:Larva / Q000276:immunology / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000276:immunology* / Q000378:metabolism; D010063:Ovum / Q000276:immunology / Q000378:metabolism*; D012313:RNA / Q000235:genetics / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D012313:RNA
keywords: Egg; Gene expression; Immune system; Ontogeny; Rainbow trout