title: Proteome analysis of hepatic non-parenchymal cells of immune liver fibrosis rats.
abstract: Elucidation of the mechanisms of liver fibrogenesis is important to treat liver fibrosis. In this study, we established rat models of liver fibrosis with stages from 0-1, 2, and 3-4 to 4 at 2, 4, 6, and 8 weeks, respectively, by injection of pig serum. Liver fibrogenesis was detected by Masson's trichrome staining. Rat non-parenchymal cells (NPCs) were enriched 4-fold by Percoll density gradient centrifugation. Protein extracts from NPCs were prepared at 4 and 8 weeks, separated by two-dimensional electrophoresis, and then stained with Coomassie Blue G-250. At 4 weeks, we identified 18 non-redundant differentially expressed proteins of which protein disulfide-isomerase associated protein 3 (PDIA3) and NDUV showed consistent expression at protein and mRNA levels from 4 to 8 weeks. PDIA3 was found to be down-regulated by Western blotting in the rat model and immunohistochemically in human liver. Our results revealed important aspects of the pathogenesis/progression of liver fibrosis and demonstrated important changes in protein expression levels of NPCs at various stages of liver fibrosis.
authors.affiliation: Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, China.
authors.forename: QianQian
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, China.
authors.forename: YanLing
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, China.
authors.forename: XiaoFang
authors.initials: X
authors.lastname: Jia
authors.affiliation: Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Yin
authors.affiliation: Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Institute of Clinical Pharmacology, Pharmacogenetics Research Institute, Central South University, Changsha, 410078, China.
authors.forename: DongSheng
authors.initials: D
authors.lastname: Ouyang
authors.affiliation: Institute of Clinical Pharmacology, Pharmacogenetics Research Institute, Central South University, Changsha, 410078, China. hhzhou@public.cs.hn.cn.
authors.forename: HongHao
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, China. zhanglijun1221@163.com.|Institute of Clinical Pharmacology, Pharmacogenetics Research Institute, Central South University, Changsha, 410078, China. zhanglijun1221@163.com.
authors.forename: LiJun
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008103:Liver Cirrhosis / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D019704:Protein Disulfide-Isomerases / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000235:genetics; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D020543:Proteome; D019704:Protein Disulfide-Isomerases