title: Microperforation of the colon: animal model in rats to reproduce mucosal thermal damage.
abstract: BACKGROUND
  The aim of the present study was to develop a rat model of colonic microperforation secondary to thermal injury for future studies to assess new treatments.
  
  
  METHODS
  Twenty-four male Sprague-Dawley rats were used in this study. Hot biopsy forceps were used for all treatments. All lesions were created in proximal left colon using the soft coagulation setting. The power setting tested was 40 W, and the durations of monopolar soft coagulation application evaluated were 2, 3, and 4 s.
  
  
  RESULTS
  In the acute phase, 48 h after thermal injury, durations of cautery of 2 and 3 s resulted in transmural necrosis, whereas with 4 s microperforation was obtained. In the late phase, 7 d after the damage, only duration of cautery of 4 s showed deep cautery effects, with signs of peritonitis.
  
  
  CONCLUSIONS
  We determined optimal power settings and duration of therapy in a rat model for producing electrocautery that involves transmural necrosis with microperforation.
authors.affiliation: Endoscopy Unit, Department of Gastroenterology, Hospital Universitari Germans Trias i Pujol, Badalona, Spain; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Barcelona, Spain. Electronic address: vlorenzo.germanstrias@gencat.cat.
authors.forename: Vicente
authors.initials: V
authors.lastname: Lorenzo-Zúñiga
authors.affiliation: Endoscopy Unit, Department of Gastroenterology, Hospital Universitari Germans Trias i Pujol, Badalona, Spain.
authors.forename: Jaume
authors.initials: J
authors.lastname: Boix
authors.affiliation: Endoscopy Unit, Department of Gastroenterology, Hospital Universitari Germans Trias i Pujol, Badalona, Spain.
authors.forename: Vicente
authors.initials: V
authors.lastname: Moreno-de-Vega
authors.affiliation: Department of Pathology, Hospital Universitari Germans Trias i Pujol, Badalona, Spain.
authors.forename: Napoleón D
authors.initials: ND
authors.lastname: de-la-Ossa
authors.affiliation: Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Barcelona, Spain; Institut Investigació en Ciències de la Salut Germans Trias i Pujol, Badalona, Spain.
authors.forename: Gemma
authors.initials: G
authors.lastname: Òdena
authors.affiliation: Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Barcelona, Spain; Institut Investigació en Ciències de la Salut Germans Trias i Pujol, Badalona, Spain.
authors.forename: Ramon
authors.initials: R
authors.lastname: Bartolí
mesh_terms: D000818:Animals; D003108:Colonic Diseases / Q000209:etiology* / Q000473:pathology; D003113:Colonoscopy / Q000009:adverse effects*; D004195:Disease Models, Animal; D004564:Electrocoagulation / Q000009:adverse effects*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
keywords: Colonoscopy; Experimental model; Rat; Thermal damage