title: Sublingual administration of a helper-dependent adenoviral vector expressing the codon-optimized soluble fusion glycoprotein of human respiratory syncytial virus elicits protective immunity in mice.
abstract: Sublingual (s.l.) immunization has been described as a convenient and safe way to induce mucosal immune responses in the respiratory and genital tracts. We constructed a helper-dependent adenoviral (HDAd) vector expressing a condon-optimized soluble fusion glycoprotein (sFsyn) of respiratory syncytial virus (HDAd-sFsyn) and explored the potential of s.l. immunization with HDAd-sFsyn to stimulate immune responses in the respiratory mucosa. The RSV specific systemic and mucosal immune responses were generated in BALB/c mice, and the serum IgG with neutralizing activity was significantly elevated after homologous boost with s.l. application of HDAd-sFsyn. Humoral immune responses could be measured even 14weeks after a single immunization. Upon challenge, s.l. immunization with HDAd-sFsyn displayed an effective protection against RSV infection. These findings suggest that s.l. administration of HDAd-sFsyn acts as an effective and safe mucosal vaccine against RSV infection, and may be a useful tool in the prevention of RSV infection.
authors.affiliation: College of Life Sciences & Bioengineering, Beijing Jiaotong University, Beijing 100044, China.
authors.forename: Yuan-hui
authors.initials: YH
authors.lastname: Fu
authors.affiliation: College of Life Sciences & Bioengineering, Beijing Jiaotong University, Beijing 100044, China.
authors.forename: Yue-Ying
authors.initials: YY
authors.lastname: Jiao
authors.affiliation: College of Life Sciences & Bioengineering, Beijing Jiaotong University, Beijing 100044, China. Electronic address: jshhe@bjtu.edu.cn.
authors.forename: Jin-sheng
authors.initials: JS
authors.lastname: He
authors.affiliation: Department of Immunology, Anhui Medical University, Hefei, Anhui 230032, China.
authors.forename: Gui-Yuan
authors.initials: GY
authors.lastname: Giang
authors.affiliation: Department of Gastrointestinal Oncological Surgery, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan 471003, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: College of Life Sciences & Bioengineering, Beijing Jiaotong University, Beijing 100044, China.
authors.forename: Yi-Fei
authors.initials: YF
authors.lastname: Yan
authors.affiliation: College of Life Sciences & Bioengineering, Beijing Jiaotong University, Beijing 100044, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Ma
authors.affiliation: College of Life Sciences & Bioengineering, Beijing Jiaotong University, Beijing 100044, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Hua
authors.affiliation: College of Life Sciences & Bioengineering, Beijing Jiaotong University, Beijing 100044, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Life Sciences & Bioengineering, Beijing Jiaotong University, Beijing 100044, China.
authors.forename: Xiang-Lei
authors.initials: XL
authors.lastname: Peng
authors.affiliation: Division of Hematology-Oncology, Mayo Clinic, Scottsdale, AZ 85259, USA.
authors.forename: Chang-Xin
authors.initials: CX
authors.lastname: Shi
authors.affiliation: College of Life Sciences & Bioengineering, Beijing Jiaotong University, Beijing 100044, China; Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Hong
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000286:Administration, Sublingual; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D004195:Disease Models, Animal; D004337:Drug Carriers / Q000008:administration & dosage*; D005260:Female; D018928:Immunity, Mucosal; D007074:Immunoglobulin G / Q000097:blood; D008807:Mice, Inbred BALB C; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology / Q000517:prevention & control*; D022261:Respiratory Syncytial Virus Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology*; D018113:Respiratory Syncytial Virus, Human / Q000235:genetics / Q000276:immunology*; D044967:Serum / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D004337:Drug Carriers; D007074:Immunoglobulin G; D022261:Respiratory Syncytial Virus Vaccines; D014614:Vaccines, Synthetic
keywords: Human respiratory syncytial virus; Immune responses; Soluble fusion glycoprotein; Sublingual immunization