title: Antitumor effect of TRAIL on oral squamous cell carcinoma using magnetic nanoparticle-mediated gene expression.
abstract: We developed a new magnetic nanovector to improve the efficiency and targeting of transgene therapy for oral squamous cell carcinoma (OSCC). Positively charged polymer PEI-modified Fe(3)O(4) magnetic nanoparticles were tested as gene transfer vectors in the presence of a magnetic field. The Fe(3)O(4) nanoparticles were prepared by a co-precipitation method and had good dispersibility in water. These nanoparticles modified by PEI were combined with negatively charged pACTERT-EGFP via electrostatic interaction. The transfection efficiency of the magnetic nano-gene vector with the magnetic field was determined by a fluorescence-inverted microscope and flow cytometry. The results showed significant improvement compared with the control group (p < 0.05). The magnetic complexes also exhibited up to 6-times higher transfection efficiency compared with commonly used PEI or lipofectin. On the basis of these results, the antitumor effect with suicide gene therapy using pACTERT-TRAIL in vitro and vivo was evaluated. In vitro apoptosis was determined with the Annexin V-FITC Apoptosis Detection Kit. The results suggested that PEI-modified Fe(3)O(4) nanoparticles could mediate the killing of Tca83 cells. Furthermore, treatment with pACTERT-TRAIL delivered by magnetic nanoparticles showed a significant cytostatic effect through the induction of apoptosis in a xenograft model. This indicates that magnetic nano-gene vectors could improve the transgene efficiency for Tca83 cells and could exhibit antitumor functions with the plasmid pACTERT-TRAIL. This may be a new way to treat OSCC.
authors.affiliation: Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing, 210008, China.
authors.forename: Leiying
authors.initials: L
authors.lastname: Miao
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.forename: Jiuyu
authors.initials: J
authors.lastname: Ge
authors.forename: Weidong
authors.initials: W
authors.lastname: Yang
authors.forename: Jinzhong
authors.initials: J
authors.lastname: Liu
authors.forename: Weibin
authors.initials: W
authors.lastname: Sun
authors.forename: Bai
authors.initials: B
authors.lastname: Yang
authors.forename: Changyu
authors.initials: C
authors.lastname: Zheng
authors.forename: Hongchen
authors.initials: H
authors.lastname: Sun
authors.forename: Qingang
authors.initials: Q
authors.lastname: Hu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D045744:Cell Line, Tumor; D004337:Drug Carriers / Q000737:chemistry*; D015870:Gene Expression; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D011094:Polyethyleneimine / Q000737:chemistry; D011401:Promoter Regions, Genetic / Q000235:genetics; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000235:genetics*; D019098:Telomerase / Q000235:genetics; D014162:Transfection
chemical_list: D004337:Drug Carriers; D058185:Magnetite Nanoparticles; D053221:TNF-Related Apoptosis-Inducing Ligand; D011094:Polyethyleneimine; D019098:Telomerase