title: Modeling of Zymomonas mobilis central metabolism for novel metabolic engineering strategies.
abstract: Mathematical modeling of metabolism is essential for rational metabolic engineering. The present work focuses on several types of modeling approach to quantitative understanding of central metabolic network and energetics in the bioethanol-producing bacterium Zymomonas mobilis. Combined use of Flux Balance, Elementary Flux Mode, and thermodynamic analysis of its central metabolism, together with dynamic modeling of the core catabolic pathways, can help to design novel substrate and product pathways by systematically analyzing the solution space for metabolic engineering, and yields insights into the function of metabolic network, hardly achievable without applying modeling tools.
authors.affiliation: Institute of Microbiology and Biotechnology, University of Latvia Riga, Latvia.
authors.forename: Uldis
authors.initials: U
authors.lastname: Kalnenieks
authors.affiliation: Department of Computer Systems, Latvia University of Agriculture Jelgava, Latvia.
authors.forename: Agris
authors.initials: A
authors.lastname: Pentjuss
authors.affiliation: Institute of Microbiology and Biotechnology, University of Latvia Riga, Latvia.
authors.forename: Reinis
authors.initials: R
authors.lastname: Rutkis
authors.affiliation: Institute of Microbiology and Biotechnology, University of Latvia Riga, Latvia ; Department of Computer Systems, Latvia University of Agriculture Jelgava, Latvia ; SIA TIBIT Jelgava, Latvia.
authors.forename: Egils
authors.initials: E
authors.lastname: Stalidzans
authors.affiliation: Department of Biological and Medical Sciences, Oxford Brookes University Oxford, UK.
authors.forename: David A
authors.initials: DA
authors.lastname: Fell
keywords: Entner–Doudoroff pathway; Zymomonas mobilis; central metabolism; elementary flux modes; kinetic modeling; metabolic engineering; stoichiometric modeling; systems biology