title: Advances in the quantification of protein microarrays.
abstract: The determination of the protein composition is a challenging task, yet a valuable endeavor. Information that lies within the composition can help predicting pathogenic mechanisms and diseases. Therefore, a mandatory requirement is the accurate quantification. Protein microarrays are a promising alternative to complement mass spectrometry (MS) techniques. Nevertheless, effects in microarray production and assay development may lead to altered signal intensities. Techniques like NormaCurve provide a more robust quantification by taking the immobilized protein content into account. Printing dilution series can be used to construct a non-linear standard curve to estimate the amount of bound IgG, which helps to overrule the detection range of one order of magnitude. Standard protocols based on projects like the 'minimum information about a proteomics experiment' will help to improve quality and overall comparability.
authors.affiliation: Fraunhofer for Biomedical Engineering IBMT, Branch Potsdam-Golm, Am Mühlenberg 13, 14476 Potsdam, Germany; Humboldt University Berlin, Department of Biology, D-10115 Berlin, Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Pratsch
authors.affiliation: Fraunhofer for Biomedical Engineering IBMT, Branch Potsdam-Golm, Am Mühlenberg 13, 14476 Potsdam, Germany.
authors.forename: Robert
authors.initials: R
authors.lastname: Wellhausen
authors.affiliation: Fraunhofer for Biomedical Engineering IBMT, Branch Potsdam-Golm, Am Mühlenberg 13, 14476 Potsdam, Germany. Electronic address: harald.seitz@ibmt.fraunhofer.de.
authors.forename: Harald
authors.initials: H
authors.lastname: Seitz
mesh_terms: D057230:Limit of Detection; D013058:Mass Spectrometry / Q000379:methods; D040081:Protein Array Analysis / Q000379:methods*; D011506:Proteins / Q000032:analysis
chemical_list: D011506:Proteins