title: [Effect of blood cooling and detoxification formula and its dismantled formulae on keratinocyte proliferation induced by activated T lymphocytes].
abstract: OBJECTIVE
  To observe the effect of the blood cooling and detoxification formula and its dismantled formulae on activated T lymphocytes-induced keratinocyte proliferation and cytokine secretion.
  
  
  METHODS
  Rat drug-containing serum was prepared. PDB-stimulated T lymphocytes and Colo-16 cells were co-cultured, then added with the drug-containing serum, and laid aside for 24 h. The cell proliferation was detected by MTT assay. Cytokines TNF-alpha, sICAM-1 and IFN-gamma were detected by ELISA.
  
  
  RESULTS
  The blood cooling and detoxification formula has a notable inhibitory effect on activated T lymphocyte-induced Colo-16 keratinocyte proliferation, with a significant difference between the whole formula group and the blood cooling group (P < 0.01). After activated T lymphocytes had added into Colo-16 cells for 24 hour, sICAM-1, TNF-alpha and IFN-gamma significantly increased. Though the whole blood cooling and detoxification formula, the blood cooling formula and the detoxification formula could notably reduce sICAM-1, TNF-alpha, IFN-gamma, the whole formula group showed the most significant effect.
  
  
  CONCLUSIONS
  The blood cooling and detoxification formula, as an effective traditional Chinese medicine compound for clinical treatment of psoriasis, can significantly inhibit activated T lymphocyte-induced keratinocyte proliferation and cytokine secretion.
authors.affiliation: Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University, Beijing Institute of Chinese Medicine, Beijing 100010, China. xiexinran@sohu.com
authors.forename: Xin-Ran
authors.initials: XR
authors.lastname: Xie
authors.affiliation: Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University, Beijing Institute of Chinese Medicine, Beijing 100010, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University, Beijing Institute of Chinese Medicine, Beijing 100010, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University, Beijing Institute of Chinese Medicine, Beijing 100010, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University, Beijing Institute of Chinese Medicine, Beijing 100010, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
chemical_list: D016207:Cytokines; D004365:Drugs, Chinese Herbal