title: Benzophenone-based photochemical micropatterning of biomolecules to create model substrates and instructive biomaterials.
abstract: The extracellular matrix (ECM) is a dynamic and heterogeneous environment that controls many aspects of cell behavior. Not surprisingly, many different approaches have focused on creating model substrates that recapitulate the biomolecular, topographical, and mechanical properties of the ECM for in vitro studies of cell behavior. This chapter details a general, versatile method for the spatially controlled deposition of multiple biomolecules onto both planar and topographically complex support structures with micrometer resolution. This approach is based upon the well-understood photochemical UV crosslinking of benzophenone (BP) to solution-phase biomolecules. This is a molecularly general strategy that can be utilized to immobilize biomolecules onto any surface prefunctionalized with BP. Examples described herein include modification of planar and corrugated glass substrates as well as collagen-glycosaminoglycan biomaterials configured either as highly porous scaffolds or nonporous membranes with a variety of biomolecular targets, including proteins, glycoproteins, and carbohydrates.
authors.affiliation: Departments of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors.forename: Aurora J
authors.initials: AJ
authors.lastname: Turgeon
authors.affiliation: Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors.forename: Brendan A
authors.initials: BA
authors.lastname: Harley
authors.affiliation: Departments of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors.forename: Ryan C
authors.initials: RC
authors.lastname: Bailey
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001577:Benzophenones / Q000737:chemistry; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods; D020099:Coated Materials, Biocompatible; D003094:Collagen / Q000737:chemistry; D005109:Extracellular Matrix / Q000502:physiology; D005353:Fibronectins / Q000378:metabolism; D005898:Glass / Q000737:chemistry; D006025:Glycosaminoglycans / Q000737:chemistry; D042381:Granulocyte Precursor Cells / Q000502:physiology*; D018922:HL-60 Cells; D006801:Humans; D019169:Jurkat Cells; D036904:Leukocyte Rolling / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000494:pharmacology; D051379:Mice; D010006:Osteoblasts / Q000502:physiology*; D019007:P-Selectin / Q000494:pharmacology; D010777:Photochemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D013314:Stress, Mechanical; D013499:Surface Properties; D013601:T-Lymphocytes / Q000502:physiology*
chemical_list: D001577:Benzophenones; D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D006025:Glycosaminoglycans; D008562:Membrane Glycoproteins; D019007:P-Selectin; C084882:P-selectin ligand protein; D011092:Polyethylene Glycols; C047723:benzophenone; D003094:Collagen
keywords: Benzophenone; Biomaterials; Biomolecular patterning; Model substrates; photolithography