title: Differentiation of Aedes atlanticus and Aedes tormentor by restriction fragment length polymorphisms of the second internal transcribed spacer.
abstract: Using novel DNA sequence data, we designed a restriction enzyme assay that distinguishes Aedes atlanticus and Ae. tormentor, based on size polymorphisms. The restriction endonuclease Hpy188I digests polymerase chain reaction-amplified 2nd internal transcribed spacer products once for Ae. atlanticus and twice for Ae. tormentor, thus providing a useful method for identifying adult female collections that are generally considered morphologically indistinguishable.
authors.affiliation: Vector-Borne Infectious Disease Laboratory, Environmental Health Sciences Program, College of Health and Human Sciences, Western Carolina University, Cullowhee, NC 28723, USA.
authors.forename: Charles B
authors.initials: CB
authors.lastname: Sither
authors.affiliation: Vector-Borne Infectious Disease Laboratory, Environmental Health Sciences Program, College of Health and Human Sciences, Western Carolina University, Cullowhee, NC 28723, USA.
authors.forename: Virginia E
authors.initials: VE
authors.lastname: Hopkins
authors.affiliation: Vector-Borne Infectious Disease Laboratory, Environmental Health Sciences Program, College of Health and Human Sciences, Western Carolina University, Cullowhee, NC 28723, USA.
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Harrison
authors.affiliation: Forensic Science Program, College of Arts and Sciences, Western Carolina University, Cullowhee, NC 28723, USA.
authors.forename: Brittania J
authors.initials: BJ
authors.lastname: Bintz
authors.affiliation: Brunswick County Mosquito Control, PO Box 249, Bolivia, NC 28422, USA.
authors.forename: E Y
authors.initials: EY
authors.lastname: Hickman
authors.affiliation: Brunswick County Mosquito Control, PO Box 249, Bolivia, NC 28422, USA.
authors.forename: Jeffrey S
authors.initials: JS
authors.lastname: Brown
authors.affiliation: Forensic Science Program, College of Arts and Sciences, Western Carolina University, Cullowhee, NC 28723, USA.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Wilson
authors.affiliation: Vector-Borne Infectious Disease Laboratory, Environmental Health Sciences Program, College of Health and Human Sciences, Western Carolina University, Cullowhee, NC 28723, USA.
authors.forename: Brian D
authors.initials: BD
authors.lastname: Byrd
mesh_terms: D000330:Aedes / Q000145:classification* / Q000235:genetics*; D000818:Animals; D021901:DNA, Intergenic; D005260:Female; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity
chemical_list: D021901:DNA, Intergenic