title: [Studies on adventitious root induction in vitro and suspension culture of Polygonum multiflorum].
abstract: To achieve sustainable resources use of Polygonum multiflorum, adventitious roots were efficiently induced and cultured by suspension culture. In order to obtain optimal medium for induction adventitious roots from the young stems of P. multiflorum, MS medium was optimized by supplementing with different concentrations of sucrose and plant growth substances. The optimal medium for suspension culture of adventitious roots was determined by orthogonal design. The adventitious roots with suspension culture were subcultured, and the growth curve was also determined. Furthermore, the effective compound in adventitious roots was detected. The result indicated that the optimal medium for efficient induction of adventitious roots was MS medium containing 4% w/v sucrose, supplemented with 2.0 mg x L(-1) NAA, and 0.2 mg x L(-1) 6-BA. The optimal medium for suspension culture of adventitious roots was MS medium containing 3% sucrose, supplemented with 2.0 mg x L(-1) NAA, and 0.2 mg x L(-1) ABT-7.2,3,5,4'-tetrahydroxyl-diphenyl-ethylene-2-O-beta-D-glucoside was detected in adventitious roots, which was effective compound in medicinal material of P. multiflorum. In conclusion, the experiment achieved efficient induction and suspension culture of adventitious roots of P. multiflorum, and laid a foundation for the research on the sustainable use of traditional Chinese medicine resources.
authors.affiliation: Anhui University of Chinese Medicine, Hefei 230031, China. huangluqi@263.net
authors.forename: He-Ping
authors.initials: HP
authors.lastname: Huang
authors.affiliation: China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Shan-Lin
authors.initials: SL
authors.lastname: Gao
authors.affiliation: Anhui University of Chinese Medicine, Hefei 230031, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: National Resoure Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Lu-Qi
authors.initials: LQ
authors.lastname: Huang
authors.affiliation: Anhui University of Chinese Medicine, Hefei 230031, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Huang
mesh_terms: D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development* / Q000378:metabolism; D019664:Polygonaceae / Q000254:growth & development* / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D003470:Culture Media; D010937:Plant Growth Regulators; D013395:Sucrose