title: [Expression and significance of DNA methyltransferase in sera of patients with lung cancer].
abstract: OBJECTIVE
  To explore the protein expressions of DNMT1, DNMT3a and DNMT3b in sera of lung cancer patients.
  
  
  METHODS
  Enzyme-linked immunosorbent assay (ELISA) method was used to measure the protein expressions of DNMT1, DNMT3a and DNMT3b in 136 lung cancer patients hospitalized at Department of Respiratory Diseases, First Affiliated Hospital, Zhengzhou University during September 2012 to June 2013. And 147 healthy controls were selected from a population of physical examination at Sixth People's Hospital of Zhengzhou. And the relationship was analyzed between protein expressions of DNMT1, DNMT3a and DNMT3b and clinic characteristics of lung cancer.
  
  
  RESULTS
  The protein expressions of DNMT1, DNMT3a and DNMT3b in patients with lung cancer (15 ± 10, 997 ± 76 , 302 ± 25) were higher than those of the controls (13 ± 10, 344 ± 93, 108 ± 22). And there were statistical significance (t = 3.28, 62.51, 37.27; P = 0.021, 0.000, 0.000). The results of Logistic regression show that the protein expressions DNMT1, DNMT3a and DNMT3b increased morbidity for lung cancer (χ(2) = 14.811, 26.768, 12.057; P = 0.000, 0.000 0.001), especially so for DNMT1 (OR = 1.545, 95%CI: 1.238-1.928). No correlation existed between the protein expressions of DNMT1, DNMT3a, DNMT3b and histological types or stages (P > 0.05).
  
  
  CONCLUSIONS
  The high protein expressions of serum DNMT1, DNMT3a and DNMT3b increase morbidity for lung cancer. And these markers may predict the early occurrence of lung cancer.
authors.affiliation: Department of Respiratory Diseases, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Yue-jing
authors.initials: YJ
authors.lastname: Feng
authors.forename: Rui
authors.initials: R
authors.lastname: Tian
authors.forename: Ai-guo
authors.initials: AG
authors.lastname: You
authors.affiliation: Email: wangjing@zzu.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Yong-jun
authors.initials: YJ
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: An-yan
authors.initials: AY
authors.lastname: Zhou
authors.forename: Xiao-ling
authors.initials: XL
authors.lastname: Wei
authors.forename: Qi-dong
authors.initials: QD
authors.lastname: He
authors.forename: Xu
authors.initials: X
authors.lastname: Feng
authors.forename: Yong-min
authors.initials: YM
authors.lastname: Yu
authors.forename: Xiao-min
authors.initials: XM
authors.lastname: Deng
mesh_terms: D000230:Adenocarcinoma / Q000097:blood* / Q000473:pathology; D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000473:pathology; D016022:Case-Control Studies; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000097:blood*; D000090205:DNA Methyltransferase 3A; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D000097584:DNA Methyltransferase 3B
chemical_list: D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; D000090205:DNA Methyltransferase 3A; D000097584:DNA Methyltransferase 3B; C000717967:DNMT3A protein, human; C000619380:DNMT1 protein, human