title: Selenite-mediated production of superoxide radical anions in A549 cancer cells is accompanied by a selective increase in SOD1 concentration, enhanced apoptosis and Se-Cu bonding.
abstract: Selenite may exert its cytotoxic effects against cancer cells via the generation of reactive oxygen species (ROS). We investigated sources of, and the cellular response to, superoxide radical anion (O2 (·-)) generated in human A549 lung cancer cells after treatment with selenite. A temporal delay was observed between selenite treatment and increases in O2 (·-) production and biomarkers of apoptosis/necrosis, indicating that the reduction of selenite by the glutathione reductase/NADPH system (yielding O2 (·-)) is a minor contributor to ROS production under these conditions. By contrast, mitochondrial and NADPH oxidase O2 (·-) generation were the major contributors. Treatment with a ROS scavenger [poly(ethylene glycol)-conjugated superoxide dismutase (SOD) or sodium 4,5-dihydroxybenzene-1,3-disulfonate] 20 h after the initial selenite treatment inhibited both ROS generation and apoptosis determined at 24 h. In addition, SOD1 was selectively upregulated and its perinuclear cytoplasmic distribution was colocalised with the cellular distribution of selenium. Interestingly, messenger RNA for manganese superoxide dismutase, catalase, inducible haem oxygenase 1 and glutathione peroxidase either remained unchanged or showed a delayed response to selenite treatment. Colocalisation of Cu and Se in these cells (Weekley et al. in J. Am. Chem. Soc. 133:18272-18279, 2011) potentially results from the formation of a Cu-Se species, as indicated by Cu K-edge extended X-ray absorption fine structure spectra. Overall, SOD1 is upregulated in response to selenite-mediated ROS generation, and this likely leads to an accumulation of toxic hydrogen peroxide that is temporally related to decreased cancer cell viability. Increased expression of SOD1 gene/protein coupled with formation of a Cu-Se species may explain the colocalisation of Cu and Se observed in these cells.
authors.affiliation: School of Chemistry and Physics, The University of Adelaide, Adelaide, SA, 5005, Australia.
authors.forename: Claire M
authors.initials: CM
authors.lastname: Weekley
authors.forename: Gloria
authors.initials: G
authors.lastname: Jeong
authors.forename: Michael E
authors.initials: ME
authors.lastname: Tierney
authors.forename: Farjaneh
authors.initials: F
authors.lastname: Hossain
authors.forename: Aung Min
authors.initials: AM
authors.lastname: Maw
authors.forename: Anu
authors.initials: A
authors.lastname: Shanu
authors.forename: Hugh H
authors.initials: HH
authors.lastname: Harris
authors.forename: Paul K
authors.initials: PK
authors.lastname: Witting
mesh_terms: D000838:Anions / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D003300:Copper / Q000737:chemistry* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D006801:Humans; D020887:Selenious Acid / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D012643:Selenium / Q000737:chemistry* / Q000378:metabolism; D013329:Structure-Activity Relationship; D013482:Superoxide Dismutase / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D000838:Anions; D013481:Superoxides; D003300:Copper; D013482:Superoxide Dismutase; D020887:Selenious Acid; D012643:Selenium