title: Antioxidant and anti-inflammatory activities of methanol extracts of Tremella fuciformis and its major phenolic acids.
abstract: Methanol extract subfractions of the edible white jelly mushroom (Tremella fuciformis), were assessed for the following antioxidant properties: ABTS(+) radical scavenging activity, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, and inhibitory activity of human low-density lipoprotein (LDL) oxidation. Among the subfractions tested, the chloroform subfraction exhibited the strongest antioxidant activity, with the highest total phenolic content (66.31 μg CAE/mg extract) and flavonoids content (5.12 μg QE/mg extract). The ABTS(+) radical scavenging activity of the chloroform subfraction was 7.89 μmol trolox/mg extract, which was the highest among all subfractions. This subfraction also showed the highest DPPH radical scavenging activity and inhibitory activity of LDL oxidation. In addition, the chloroform subfraction demonstrated anti-inflammatory activity through inhibition of nitric oxide production and inducible nitric oxide synthase expression in RAW 264.7 cells. Major phenolic acids from the mushroom extract were identified as 4-hydroxybenzoic acid (323 mg/kg dry weight of mushroom), gentisic acid (174 mg/kg dry weight of mushroom), and 4-coumaric acid (30 mg/kg dry weight of mushroom).
authors.affiliation: Dept. of Food Science and Technology, Chung-Ang Univ., Gyeonggi, 456-756, South Korea.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Hee-Seok
authors.initials: HS
authors.lastname: Lee
authors.forename: Su-Hwan
authors.initials: SH
authors.lastname: Kim
authors.forename: BoKyung
authors.initials: B
authors.lastname: Moon
authors.forename: Chan
authors.initials: C
authors.lastname: Lee
mesh_terms: D000363:Agaricales; D000893:Anti-Inflammatory Agents / Q000032:analysis / Q000494:pharmacology*; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D001487:Basidiomycota / Q000737:chemistry*; D052160:Benzothiazoles / Q000378:metabolism; D001688:Biological Products / Q000737:chemistry / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D002460:Cell Line; D003373:Coumaric Acids / Q000032:analysis / Q000494:pharmacology; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology*; D005841:Gentisates / Q000032:analysis / Q000494:pharmacology; D006801:Humans; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010226:Parabens / Q000032:analysis / Q000494:pharmacology; D010636:Phenols / Q000032:analysis / Q000494:pharmacology*; D010851:Picrates / Q000378:metabolism; D011422:Propionates; D013451:Sulfonic Acids / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D052160:Benzothiazoles; D001688:Biological Products; D001713:Biphenyl Compounds; D003373:Coumaric Acids; D005419:Flavonoids; D005841:Gentisates; D008077:Lipoproteins, LDL; D010226:Parabens; D010636:Phenols; D010851:Picrates; D011422:Propionates; D013451:Sulfonic Acids; C099252:oxidized low density lipoprotein; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D009569:Nitric Oxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D052247:Nitric Oxide Synthase Type II; C495469:p-coumaric acid; C038193:4-hydroxybenzoic acid; C010925:2,5-dihydroxybenzoic acid
keywords: anti-inflammatory activity; antioxidant activity; inducible nitric oxide synthase (iNOS); phenolic acid; white jelly mushroom (Tremella fuciformis)