title: [Systematic evaluation of retention behavior of carbohydrates in hydrophilic interaction liquid chromatography].
abstract: A systematic evaluation of retention behavior of carbohydrates in hydrophilic interaction liquid chromatography (HILIC) was performed. The influences of mobile phase, stationary phase and buffer salt on the retention of carbohydrates were investigated. According to the results, the retention time of carbohydrates decreased as the proportion of acetonitrile in mobile phase decreased. Increased time of carbohydrates was observed as the concentration of buffer salt in mobile phase increased. The retention behavior of carbohydrates was also affected by organic solvent and HILIC stationary phase. Furthermore, an appropriate retention equation was used in HILIC mode. The retention equation lnk = a + blnC(B) + cC(B) could quantitatively describe the retention factors of carbohydrates of plant origin with good accuracy: the relative error of the predicted time to actual time was less than 0.3%. The evaluation results could provide guidance for carbohydrates to optimize the experimental conditions in HILIC method development especially for carbohydrate separation
authors.affiliation: School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Fu
authors.affiliation: Yingkou Port Group Co., Ltd., Yingkou 115007, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Tu
authors.initials: T
authors.lastname: Liang
authors.affiliation: School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Xu
authors.affiliation: School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Jin
mesh_terms: D002021:Buffers; D002241:Carbohydrates / Q000737:chemistry*; D002853:Chromatography, Liquid; D057927:Hydrophobic and Hydrophilic Interactions; D009930:Organic Chemicals; D012492:Salts
chemical_list: D002021:Buffers; D002241:Carbohydrates; D009930:Organic Chemicals; D012492:Salts