title: Increased peripheral and local soluble FGL2 in the recovery of renal ischemia reperfusion injury in a porcine kidney auto-transplantation model.
abstract: BACKGROUND
  Regulatory T cells (Treg) protect kidney against ischemia reperfusion (IR) injury via suppressing innate immunity, but the mechanism has not been fully clarified. Soluble fibrinogen-like protein 2 (sFGL2), a novel effector of Treg, may affect apoptosis and inflammation. This study investigated the role of sFGL2 in renal IR injury in a porcine kidney auto-transplantation model.
  
  
  METHODS
  The left kidney was retrieved from mini pigs and infused by University of Wisconsin solution into the renal artery with the renal artery and vein clamped for 24-h cold storage. After the right nephrectomy, the left kidney was auto-transplanted into the right for 2 weeks. 3 pigs were sacrificed at day 2, 5, 7, 10 and 14 post-transplantation respectively. Collected renal tissues and daily blood samples were stored for further analyses.
  
  
  RESULTS
  Both serum creatinine and blood urea nitrogen were maximized during day 2 to 5 and followed by a gradual recovery over 2 weeks. The similar pattern were showed in histological damage, myeloperoxidase + cells and apoptosis in the kidney, as well as circulating TNF-α and IFN-γ. Serum sFGL2 presented a fluctuating increase and reached a peak at day 10. The expression of sFGL2 and its receptor FcγRIIB as well as Foxp3 and IL-10 in the kidney was notably increased from day 5 to 10.
  
  
  CONCLUSIONS
  The increased sFGL2 together with FcγRIIB during renal recovery after IR injury suggested that sFGL2 might be a potential renoprotective mediator involved in the renal self-repairing and remodeling in this 2-week porcine auto-transplantation model.
authors.forename: Zitong
authors.initials: Z
authors.lastname: Zhao
authors.forename: Cheng
authors.initials: C
authors.lastname: Yang
authors.forename: Long
authors.initials: L
authors.lastname: Li
authors.forename: Tian
authors.initials: T
authors.lastname: Zhao
authors.forename: Lingyan
authors.initials: L
authors.lastname: Wang
authors.forename: Ruiming
authors.initials: R
authors.lastname: Rong
authors.affiliation: Department of Urology, Zhongshan Hospital, Fudan University; Shanghai Key Laboratory of Organ Transplantation, 180 Fenglin Road, Shanghai 200032, China. by5@le.ac.uk.
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.forename: Ming
authors.initials: M
authors.lastname: Xu
authors.forename: Tongyu
authors.initials: T
authors.lastname: Zhu
mesh_terms: D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D004195:Disease Models, Animal; D005340:Fibrinogen / Q000378:metabolism*; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D007249:Inflammation / Q000473:pathology; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism; D007668:Kidney / Q000201:enzymology / Q000473:pathology* / Q000503:physiopathology*; D007677:Kidney Function Tests; D016030:Kidney Transplantation; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017452:Receptors, IgG / Q000378:metabolism; D020127:Recovery of Function; D015427:Reperfusion Injury / Q000097:blood* / Q000473:pathology / Q000503:physiopathology* / Q000628:therapy; D012995:Solubility; D014182:Transplantation, Autologous
chemical_list: C080571:Fc gamma receptor IIB; D051858:Forkhead Transcription Factors; D012333:RNA, Messenger; D017452:Receptors, IgG; D016753:Interleukin-10; D005340:Fibrinogen; D053148:Caspase 3