title: Purification and evaluation of the enzymatic properties of a novel fructosyltransferase from Aspergillus oryzae: a potential biocatalyst for the synthesis of sucrose 6-acetate.
abstract: A novel fructosyltransferase (AoFT) capable of synthesizing sucrose 6-acetate (S6A) from sucrose and glucose 6-acetate has been purified to homogeneity from Aspergillus oryzae ZZ-01. Its molecular mass was ~50 kDa by SDS-PAGE; optimal activity was at 45 °C and it was stable from pH 4.5 to 7.5 with an optimum pH of 6. Mg(2+), K(+) (5 mM), propanol, toluene (50%, v/v), Tween 20 or Triton X-100 (1%, w/v) increased the transfructosylation activity by 20, 17, 17, 10, 25 and 20%, respectively. An overall conversion of 32% was achieved under optimal conditions over 24 h. This is the first report that the purified and characterized the fructosyltransferase from Aspergillus capable of synthesis of S6A from sucrose and glucose 6-acetate.
authors.affiliation: School of Food and Biological Engineering, Zhengzhou University of Light Industry, 5 Dongfeng Rd, Zhengzhou, 450002, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wei
authors.forename: Xuan
authors.initials: X
authors.lastname: Yu
authors.forename: Yingying
authors.initials: Y
authors.lastname: Wang
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Zhu
authors.forename: Congcong
authors.initials: C
authors.lastname: Du
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Jia
authors.forename: Duobin
authors.initials: D
authors.lastname: Mao
mesh_terms: D000595:Amino Acid Sequence; D001236:Aspergillus oryzae / Q000201:enzymology*; D004795:Enzyme Stability; D006602:Hexosyltransferases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D013395:Sucrose / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D001426:Bacterial Proteins
chemical_list: D006602:Hexosyltransferases; D013395:Sucrose; C556997:sucrose-6-acetate; C021451:inulosucrase; D001426:Bacterial Proteins