title: Evaluation of the stability of an SR398/GroES chaperonin complex.
abstract: The stability of an SR398/GroES chaperonin complex was examined. As was expected, based on the finding of previous studies, the SR398/GroES complex was extremely stable in the presence of an excess amount of free adenosine 5'-[γ-thio]triphosphate (ATPγS) or adenosine 5'-(β,γ-imido)triphosphate (AMPPNP). However, the complex was not stable in the absence of nucleotides. These results indicate that ATPγS and AMPPNP repeatedly associated to and dissociated from the complex in a non-cooperative manner. This nucleotide exchange did not induce the dissociation of GroES and substrate from SR398, suggesting the importance of the cooperative dissociation of nucleotides from the cis-ring to release GroES and substrate proteins in the GroEL/GroES reaction cycle.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida-Shimoadachi, Sakyo-ku, Kyoto 606-8501; Department of Biotechnology, Graduate School of Engineering, Tottori University, 4-101 Koyama-Minami, Tottori 680-8552; and Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita 565-0871, Japan.
authors.forename: So
authors.initials: S
authors.lastname: Ishino
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kawata
authors.forename: Takahisa
authors.initials: T
authors.lastname: Ikegami
authors.forename: Katsumi
authors.initials: K
authors.lastname: Matsuzaki
authors.forename: Masaru
authors.initials: M
authors.lastname: Hoshino
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D018835:Chaperonin 10 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018834:Chaperonin 60 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics; D018832:Molecular Chaperones / Q000737:chemistry / Q000378:metabolism; D046912:Multiprotein Complexes / Q000737:chemistry; D009154:Mutation; D009711:Nucleotides / Q000737:chemistry; D055550:Protein Stability
chemical_list: D018835:Chaperonin 10; D018834:Chaperonin 60; D029968:Escherichia coli Proteins; D018832:Molecular Chaperones; D046912:Multiprotein Complexes; D009711:Nucleotides; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C022571:adenosine 5'-O-(3-thiotriphosphate); D000255:Adenosine Triphosphate
keywords: GroE; chaperonin; cis-ternary complex; molecular chaperone; nucleotide exchange