title: Molecular evidence of RNA editing in Bombyx chemosensory protein family.
abstract: Chemosensory proteins (CSPs) are small scavenger proteins that are mainly known as transporters of pheromone/odor molecules at the periphery of sensory neurons in the insect antennae and in the producing cells from the moth female pheromone gland. Sequencing cDNAs of RNA encoding CSPs in the antennae, legs, head, pheromone gland and wings from five single individual adult females of the silkworm moth Bombyx mori showed that they differed from genomic sequences by subtle nucleotide replacement (RDD). Both intronless and intronic CSP genes expressed RDDs, although in different rates. Most interestingly, in our study the degree of RDDs in CSP genes were found to be tissue-specific. The proportion of CSP-RDDs was found to be significantly much higher in the pheromone gland. In addition, Western blot analysis of proteins in different tissues showed existence of multiple CSP protein variant chains particularly found in the pheromone gland. Peptide sequencing demonstrated the occurrence of a pleiad of protein variants for most of all BmorCSPs from the pheromone gland. Our findings show that RNA editing is an important feature in the expression of CSPs and that a high variety of RDDs is found to expand drastically thus altering the repertoire of CSP proteins in a tissue-specific manner.
authors.affiliation: Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong Province, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Xuan
authors.affiliation: Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong Province, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Bu
authors.affiliation: Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong Province, China.
authors.forename: Yan Yan
authors.initials: YY
authors.lastname: Liu
authors.affiliation: Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong Province, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Yang
authors.affiliation: Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong Province, China.
authors.forename: Guo Xia
authors.initials: GX
authors.lastname: Liu
authors.affiliation: Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong Province, China.
authors.forename: Zhong Xue
authors.initials: ZX
authors.lastname: Fan
authors.affiliation: Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong Province, China.
authors.forename: Yu Ping
authors.initials: YP
authors.lastname: Bi
authors.affiliation: Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong Province, China.
authors.forename: Lian Qun
authors.initials: LQ
authors.lastname: Yang
authors.affiliation: Shandong Silkworm Institute, Shandong Academy of Agricultural Sciences, Yantai, Shandong Province, China.
authors.forename: Qi Nian
authors.initials: QN
authors.lastname: Lou
authors.affiliation: Institute of Computer Science, University of Tartu, Tartu, Tartumaa Province, Estonia.
authors.forename: Balaji
authors.initials: B
authors.lastname: Rajashekar
authors.affiliation: Proteomics Core Facility, Institute of Technology, University of Tartu, Tartu, Tartumaa Province, Estonia.
authors.forename: Getter
authors.initials: G
authors.lastname: Leppik
authors.affiliation: Proteomics Core Facility, Institute of Technology, University of Tartu, Tartu, Tartumaa Province, Estonia.
authors.forename: Sergo
authors.initials: S
authors.lastname: Kasvandik
authors.affiliation: Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong Province, China.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Picimbon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D057506:Arthropod Antennae / Q000378:metabolism; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary; D005260:Female; D049750:Genome, Insect; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010675:Pheromones / Q000235:genetics* / Q000378:metabolism; D011110:Polymorphism, Genetic; D017393:RNA Editing; D018035:Receptors, Odorant / Q000235:genetics / Q000378:metabolism; D012543:Scent Glands / Q000378:metabolism; D016415:Sequence Alignment; D014921:Wings, Animal / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D019476:Insect Proteins; D010675:Pheromones; D018035:Receptors, Odorant