title: Role of high expression of IL-9 in prognosis of CLL.
abstract: Chronic lymphocytic leukemia (CLL) is a common leukemia in adults, but its pathogenesis is still poorly understood. Interleukin-9 (IL-9) is initially described as a growth factor secreted by helper T cells. Recently, the oncogenic activities of IL-9 were reported in some leukemia but not chronic lymphocytic leukemia (CLL). The purpose of the present study is to investigate the expression of IL-9 from patients with CLL and to evaluate its correlation with clinical characteristics. Serum and peripheral blood mononuclear cells (PBMCs) from patients with CLL were analyzed using ELISA, RT-PCR, and western blot. ELISA analysis indicated IL-9 could be detected in 20 of 47 sera from CLL patients while none serum sample from healthy volunteers contained detectable levels of IL-9. There was a higher expression of IL-9 within PBMCs from patients with CLL compared with B cells of healthy blood donors using RT-PCR and western blot. The upregulated IL-9 was correlated to the clinical staging, ZAP-70 expression, β2 microglobulin expression and IgVH status of CLL patients (P<0.05). Our findings suggest that overexpression of IL-9 may contribute to the pathogenesis of CLL and is associated with some adverse prognostic parameters.
authors.affiliation: Department of Hematology, Provincial Hospital Affiliated to Shandong University Jinan, Shandong, 250021, China.
authors.forename: Na
authors.initials: N
authors.lastname: Chen
authors.affiliation: Department of Hematology, Provincial Hospital Affiliated to Shandong University Jinan, Shandong, 250021, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Lv
authors.affiliation: Department of Hematology, Provincial Hospital Affiliated to Shandong University Jinan, Shandong, 250021, China.
authors.forename: Peipei
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Hematology, Provincial Hospital Affiliated to Shandong University Jinan, Shandong, 250021, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Lu
authors.affiliation: Department of Hematology, Provincial Hospital Affiliated to Shandong University Jinan, Shandong, 250021, China ; Institute of Diagnostics, Shandong University, School of Medicine Jinan, Shandong, 250012, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D015153:Blotting, Western; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D016906:Interleukin-9 / Q000097:blood* / Q000235:genetics; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood / Q000235:genetics / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D012333:RNA, Messenger / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation; D051746:ZAP-70 Protein-Tyrosine Kinase / Q000097:blood
chemical_list: D014408:Biomarkers, Tumor; C508607:IL9 protein, human; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D016906:Interleukin-9; D012333:RNA, Messenger; D051746:ZAP-70 Protein-Tyrosine Kinase; C497261:ZAP70 protein, human
keywords: IL-9; chronic lymphocytic leukemia; prognosis