title: Diverse expression levels of two codon-optimized genes that encode human papilloma virus type 16 major protein L1 in Hansenula polymorpha.
abstract: Two versions of an optimized gene that encodes human papilloma virus type 16 major protein L1 were designed according to the codon usage frequency of Pichia pastoris. Y16 was highly expressed in both P. pastoris and Hansenula polymorpha. M16 expression was as efficient as that of Y16 in P. pastoris, but merely detectable in H. polymorpha even though transcription levels of M16 and Y16 were similar. H. polymorpha had a unique codon usage frequency that contains many more rare codons than Saccharomyces cerevisiae or P. pastoris. These findings indicate that even codon-optimized genes that are expressed well in S. cerevisiae and P. pastoris may be inefficiently expressed in H. polymorpha; thus rare codons must be avoided when universal optimized gene versions are designed to facilitate expression in a variety of yeast expression systems, especially H. polymorpha is involved.
authors.affiliation: Department of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Yunnan Engineering Research Center of Vaccine Research & Development on Severe Infectious Diseases, #935 Jiaoling Road, Kunming, 650118, Yunnan, People's Republic of China, cunbao_liu@163.com.
authors.forename: Cunbao
authors.initials: C
authors.lastname: Liu
authors.forename: Xu
authors.initials: X
authors.lastname: Yang
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Yao
authors.forename: Weiwei
authors.initials: W
authors.lastname: Huang
authors.forename: Wenjia
authors.initials: W
authors.lastname: Sun
authors.forename: Yanbing
authors.initials: Y
authors.lastname: Ma
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001709:Biotechnology; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003062:Codon; D018628:Gene Dosage; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D012343:RNA, Transfer / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D036022:Capsid Proteins; D003062:Codon; D009856:Oncogene Proteins, Viral; D011994:Recombinant Proteins; C063317:L1 protein, Human papillomavirus type 16; D012343:RNA, Transfer