title: Galactosylated collagen matrix enhanced in vitro maturation of human embryonic stem cell-derived hepatocyte-like cells.
abstract: Due to their important biomedical applications, functional human embryonic stem cell-derived hepatocyte-like cells (hESC-HLCs) are an attractive topic in the field of stem cell differentiation. Here, we have initially differentiated hESCs into functional hepatic endoderm (HE) and continued the differentiation by replating them onto galactosylated collagen (GC) and collagen matrices. The differentiation of hESC-HE cells into HLCs on GC substrate showed significant up-regulation of hepatic-specific genes such as ALB, HNF4α, CYP3A4, G6P, and ASGR1. There was more albumin secretion and urea synthesis, as well as more cytochrome p450 activity, in differentiated HLCs on GC compared to the collagen-coated substrate. These results suggested that GC substrate has the potential to be used for in vitro maturation of hESC-HLCs.
authors.affiliation: Department of Stem Cells and Developmental Biology at the Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, P.O. Box 19395-4644, Tehran, Iran.
authors.forename: Arefeh
authors.initials: A
authors.lastname: Ghodsizadeh
authors.forename: Hossein
authors.initials: H
authors.lastname: Hosseinkhani
authors.forename: Abbas
authors.initials: A
authors.lastname: Piryaei
authors.forename: Behshad
authors.initials: B
authors.lastname: Pournasr
authors.forename: Mostafa
authors.initials: M
authors.lastname: Najarasl
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Hiraoka
authors.forename: Hossein
authors.initials: H
authors.lastname: Baharvand
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects; D060846:Cell Engineering / Q000379:methods; D003094:Collagen / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D005690:Galactose / Q000737:chemistry / Q000378:metabolism; D020869:Gene Expression Profiling; D022781:Hepatocytes / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D006801:Humans
chemical_list: D003094:Collagen; D005690:Galactose