title: Development of an ultrasensitive aptasensor for the detection of aflatoxin B1.
abstract: Contamination of feed and food by aflatoxin B1 (AFB1), one of the most toxic of the mycotoxins, is a global concern. To prevent food safety scares, and avoid subsequent economic losses due to the recall of contaminated items, methods for the rapid, sensitive and specific detection of AFB1 at trace levels are much in demand. In this work, a simple, ultrasensitive, and reliable aptasensor is described for the detection of AFB1. An AFB1 aptamer was used as a molecular recognition probe, while its complementary DNA played a role as a signal generator for amplification by real-time quantitative polymerase chain reaction (PCR). Under optimal conditions, a wide linear detection range (5.0 × 10(-5) to 5.0 ng mL(-1)) was achieved, with a high sensitivity (limit of detection (LOD)=25 fg mL(-1)). In addition, the proposed aptasensor exhibited excellent specificity for AFB1 compared with eight other mycotoxins, with no obvious Ct value change. This aptasensor can also be used in quantifying AFB1 levels in Chinese wild rye hay samples and infant rice cereal samples, demonstrating satisfactory recoveries in the range of 88-127% and 94-119%, respectively. This detection technique has a significant potential for high-throughput, quantitative determination of mycotoxin levels in a large range of feeds and foods.
authors.affiliation: Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; College of Animal Science and Technology, Xinjiang Agricultural University, Urumchi 830000, PR China; Ministry of Agriculture-Milk and Dairy Product Inspection Center (Beijing), Beijing 100193, PR China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Guo
authors.affiliation: Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; Ministry of Agriculture-Milk and Dairy Product Inspection Center (Beijing), Beijing 100193, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wen
authors.affiliation: Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; Ministry of Agriculture-Milk and Dairy Product Inspection Center (Beijing), Beijing 100193, PR China; State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China. Electronic address: wangjiaqinmqc@126.com.
authors.forename: Nan
authors.initials: N
authors.lastname: Zheng
authors.affiliation: College of Animal Science and Technology, Xinjiang Agricultural University, Urumchi 830000, PR China.
authors.forename: Qiujiang
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; Ministry of Agriculture-Milk and Dairy Product Inspection Center (Beijing), Beijing 100193, PR China.
authors.forename: Haiwei
authors.initials: H
authors.lastname: Wang
authors.affiliation: Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; Ministry of Agriculture-Milk and Dairy Product Inspection Center (Beijing), Beijing 100193, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; Ministry of Agriculture-Milk and Dairy Product Inspection Center (Beijing), Beijing 100193, PR China.
authors.forename: Songli
authors.initials: S
authors.lastname: Li
authors.affiliation: Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; Ministry of Agriculture-Milk and Dairy Product Inspection Center (Beijing), Beijing 100193, PR China; State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Wang
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis*; D000821:Animal Feed / Q000382:microbiology; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D002523:Edible Grain / Q000382:microbiology; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology / Q000379:methods; D057230:Limit of Detection; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
chemical_list: D052157:Aptamers, Nucleotide; D016604:Aflatoxin B1
keywords: Aflatoxin B(1); Aptasensor; Feed and food safety; RT-qPCR