title: Comparison of a transplant multiplex viral panel on the ICEPlex system with real-time PCR for detection of cytomegalovirus, Epstein-Barr virus, and BK virus in clinical specimens.
abstract: We compared a multiplex viral transplant panel on the ICEPlex system to real-time PCR for the detection of cytomegalovirus (CMV), Epstein-Barr virus (EBV), and BK virus (BKV). The sensitivities of the ICEPlex were 83.3%, 95.5%, and 65.5% for the detection of CMV, EBV, and BKV, respectively. Interestingly, the multiplex assay detected dual infections in 16/280 (5.7%) samples tested.
authors.affiliation: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.
authors.forename: Reeti
authors.initials: R
authors.lastname: Khare
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Espy
authors.forename: Jayawant N
authors.initials: JN
authors.lastname: Mandrekar
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Binnicker
mesh_terms: D001739:BK Virus / Q000235:genetics*; D003587:Cytomegalovirus / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000821:virology; D004279:DNA, Viral / Q000235:genetics; D020031:Epstein-Barr Virus Infections / Q000175:diagnosis / Q000821:virology; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D027601:Polyomavirus Infections / Q000175:diagnosis / Q000821:virology; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D014412:Tumor Virus Infections / Q000175:diagnosis
chemical_list: D004279:DNA, Viral