title: Generation of a monoclonal antibody for INI1/hSNF5/BAF47.
abstract: INI1/hSNF5/BAF47, which has an SNF5 domain, belongs to the SWI/SNF family. This family is known as ATP-dependent regulators of gene expression by remodeling chromatin structure during cell differentiation. However, the detailed function of INI1/hSNF5/BAF47 is unclear. Here we report the generation of a specific monoclonal antibody for INI1/hSNF5/BAF47 by the mouse iliac lymph node method. The obtained antibody recognized two isoforms of INI1/hSNF5/BAF47 in immunoblotting and precisely recognized the nuclear localization of INI1/hSNF5/BAF47 in immunostaining. This antibody can contribute to further elucidation of the mechanisms of gene expression regulation by INI1/hSNF5/BAF47 during cell differentiation.
authors.affiliation: 1 Department of Advanced Medical Initiatives, JST-CREST, Faculty of Medicine, Kyushu University , Fukuoka, Japan .
authors.forename: Akihito
authors.initials: A
authors.lastname: Harada
authors.forename: Masayasu
authors.initials: M
authors.lastname: Hayashi
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Kuniyoshi
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Semba
authors.forename: Satoko
authors.initials: S
authors.lastname: Sugahara
authors.forename: Taro
authors.initials: T
authors.lastname: Tachibana
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Ohkawa
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Fujita
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology*; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D045744:Cell Line, Tumor; D002868:Chromosomal Proteins, Non-Histone / Q000276:immunology*; D004268:DNA-Binding Proteins / Q000276:immunology*; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics / Q000276:immunology; D005982:Glutathione Transferase / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D006825:Hybridomas / Q000166:cytology / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D051379:Mice; D008813:Mice, Inbred ICR; D020033:Protein Isoforms / Q000235:genetics / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000235:genetics; D000071796:SMARCB1 Protein; D014157:Transcription Factors / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D020033:Protein Isoforms; D011993:Recombinant Fusion Proteins; D000071796:SMARCB1 Protein; C513266:SMARCB1 protein, human; D014157:Transcription Factors; D005982:Glutathione Transferase