title: Inhibitory effect of Lycium barbarum polysaccharides on cell apoptosis and senescence is potentially mediated by the p53 signaling pathway.
abstract: Lycium barbarum (L. barbarum) fruit or extract has been regarded as a superior-grade Chinese medicine, used to modulate body immunity and for anti-aging purposes. However, the underlying molecular mechanisms behind these effects remain unclear. In the present study, L. barbarum polysaccharides (LBPs), considered a major contributor of L. barbarum effects, were used to elucidate its mechanism of action by phenotypic and senescence associated-β-galactosidase (SA-β-gal) assays, evaluation of survival rates in vivo and expression profiling of genes related to the p53 signaling pathway in a zebrafish model. Zebrafish embryos were continuously exposed to various concentrations of LBPs (1.0, 2.0, 3.0 and 4.0 mg/ml) for 3 days. The results of fluorescent acridine orange and SA-β-gal staining indicated that cell apoptosis and senescence mainly occur in the head at 24 hours post fertilization (hpf) and 72 hpf. In addition, resistance to replicative senescence was observed at low doses of LBPs, especially at the 3.0 mg/ml concentration. Furthermore, the expression of genes that relate to aging, such as p53, p21 and Bax, was decreased, while that of Mdm2 and TERT genes was increased after treatment with LBPs. The results demonstrated that the effects of LBPs on cell apoptosis and aging might be mediated by the p53-mediated pathway.
authors.affiliation: College of Life Science, Tonghua Normal University, Tonghua, Jilin 134002, P.R. China.
authors.forename: Guangqing
authors.initials: G
authors.lastname: Xia
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, P.R. China.
authors.forename: Nian
authors.initials: N
authors.lastname: Xin
authors.affiliation: College of Life Science, Tonghua Normal University, Tonghua, Jilin 134002, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: College of Life Science, Tonghua Normal University, Tonghua, Jilin 134002, P.R. China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Yao
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Hou
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Qi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001681:Biological Assay; D016923:Cell Death / Q000187:drug effects; D016922:Cellular Senescence / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000633:toxicity; D004625:Embryo, Nonmammalian / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D047108:Embryonic Development / Q000187:drug effects; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D010641:Phenotype; D015398:Signal Transduction / Q000187:drug effects* / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D016159:Tumor Suppressor Protein p53; C500223:lycium barbarum polysaccharide; D001616:beta-Galactosidase