title: Diversity of enterobacteria including β-lactamase producing isolates associated with the Spanish slug (Arion vulgaris).
abstract: The aim of this study was to determine diversity in members of the family Enterobacteriaceae including the beta-lactamase producing isolates associated with the Spanish slug (Arion vulgaris). 81 isolates, each representing a distinct colonial morphotype, were obtained from fecal samples of Spanish slugs and analyzed. Genetically heterogeneous or similar groups were assessed among the isolates by enterobacterial repetitive intergenic consensus (ERIC)-PCR and further characterized by 16S rDNA sequence analysis. Representative strains were allocated to the genera Citrobacter, Raoultella, Escherichia, Klebsiella, Kluyvera, and Buttiauxella. Fifteen isolates, most closely related to Citrobacter freundii and Escherichia coli, displayed an AmpC phenotype, one E. coli isolate showed an ESBL phenotype. These isolates were phenotypically and genotypically characterized by their antimicrobial resistance pattern. Phylogenetic background and multi-locus sequence typing (MLST) of the E. coli isolates were determined. The multi-drug resistant TEM-1 E. coli positive isolate belongs to the phylogenetic group A and ST10. The CMY-2 positive E. coli isolate belongs to the phylogenetic group D and ST117. Our results show that the common garden slug represents an important potential vector of β-lactamase producing Enterobacteriaceae.
authors.affiliation: Research Institute of Wildlife Ecology, Dept. of Integrative Biology and Evolution, University of Veterinary Medicine, Vienna, Savoyenstraße 1, 1160 Vienna, Austria. Electronic address: gabrielle.stalder@fiwi.at.
authors.forename: Gabrielle L
authors.initials: GL
authors.lastname: Stalder
authors.affiliation: Division for Clinical Microbiology and Infectious Biology, Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine, Vienna, Veterinärplatz 1, 1210 Vienna, Austria.
authors.forename: Igor
authors.initials: I
authors.lastname: Loncaric
authors.affiliation: Research Institute of Wildlife Ecology, Dept. of Integrative Biology and Evolution, University of Veterinary Medicine, Vienna, Savoyenstraße 1, 1160 Vienna, Austria.
authors.forename: Chris
authors.initials: C
authors.lastname: Walzer
mesh_terms: D000818:Animals; D004755:Enterobacteriaceae / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D049851:Gastropoda / Q000382:microbiology*; D014644:Genetic Variation; D010802:Phylogeny; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D001618:beta-Lactamases
keywords: AmpC; Arion vulgaris; ESBL; Multi-drug resistance; Spanish slug; β-Lactamase