title: Identification of novel tumor suppressor genes down-regulated in recurrent nasopharyngeal cancer by DNA microarray.
abstract: The nasopharyngeal cancer is a common cancer among southern Chinese. In order to better understand molecular mechanism of recurrent nasopharyngeal cancer (rNPC), we used DNA microarray to identify down-regulated tumor suppressed genes (TSGs) in rNPC, and bioinformatics to analyze their chromosomal localizations and molecular functions. Eight non-recurrent nasopharyngeal cancer (nNPC) and six rNPC tissue samples were selected, and Affymetrix Gene1.0 ST chips were used to construct the expression profiling of each tissue sample. Identify the down-regulated TSGs in rNPC by comparing expression profiling data of two type tissue samples. A total of five TSGs were identified to be down-regulated in rNPC. These five TSGs include SERPINF1, TPD52L1, FBLN1, RASSF6, and S100A2, and Signal Log Ratio were -2.2, -2.3, -3.5, -3.9 and -6.9 respectively. Chromosomal localization analysis showed that S100A2, RASSF6, TPD52L1, SERPINF1, and FBLN1 were located on chromosomes 1q, 4q, 6q, 17p and 22q, respectively. Functional analysis showed that SERPINF1 and TPD52L1 belonged to enzyme activity genes, S100A2 and FBLN1 belonged to calcium ion binding genes, RASSF6 belong to protein binding genes. Five TSGs likely to be the candidate TSGs involved in rNPC, and may play important roles in occurrence of rNPC. Chromosomes 1q, 4q, 6q, 17p and 22q may be considered as important region for screening TSGs that may relevant to rNPC. Those genes and chromosomal region need to be further studied.
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital of Wenzhou Medical College, 2 Fuxue Road, Wenzhou, 32500 Zhejiang People's Republic of China.
authors.forename: Zhenxiao
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Department of Radiotherapy, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou, 32500 Zhejiang People's Republic of China.
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital of Wenzhou Medical College, 2 Fuxue Road, Wenzhou, 32500 Zhejiang People's Republic of China.
authors.forename: Sen
authors.initials: S
authors.lastname: Lin
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital of Wenzhou Medical College, 2 Fuxue Road, Wenzhou, 32500 Zhejiang People's Republic of China.
authors.forename: Xiaobi
authors.initials: X
authors.lastname: Fang
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital of Wenzhou Medical College, 2 Fuxue Road, Wenzhou, 32500 Zhejiang People's Republic of China.
authors.forename: Chunhong
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital of Wenzhou Medical College, 2 Fuxue Road, Wenzhou, 32500 Zhejiang People's Republic of China.
authors.forename: Zhisu
authors.initials: Z
authors.lastname: Liao
keywords: Chromosomal localization; DNA microarray; Gene expression; Molecular function; Recurrent nasopharyngeal cancer; Tumor suppressor genes