title: Optimization of the procedure for extracting nucleic acids from aloe.
abstract: Aloe, an important folk herbal drug, includes abundant polysaccharides and secondary metabolites, which make it difficult to isolate high-quality DNA or RNA. In this paper, one and two improved methods were used to isolate the genomic DNA and RNA from the leaf of aloe, respectively. The obtained samples presented good quality and integrity; thus, they could be further used for many downstream molecular experiments. These reported protocols for DNA and RNA extraction offered a valuable reference for other related studies.
authors.affiliation: College of Life Science, China West Normal University, Nanchong City, China.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Life Science, China West Normal University, Nanchong City, China.
authors.forename: Y S
authors.initials: YS
authors.lastname: Zhang
authors.affiliation: Library, China West Normal University, Nanchong City, China.
authors.forename: C M
authors.initials: CM
authors.lastname: Liao
authors.affiliation: College of Life Science, China West Normal University, Nanchong City, China liuxiaohong0817@aliyun.com.
authors.forename: X H
authors.initials: XH
authors.lastname: Liu
mesh_terms: D000504:Aloe / Q000737:chemistry*; D005591:Chemical Fractionation / Q000379:methods*; D018744:DNA, Plant / Q000302:isolation & purification*; D018749:RNA, Plant / Q000302:isolation & purification*
chemical_list: D018744:DNA, Plant; D018749:RNA, Plant