title: In vitro assessment of soybean lecithin and egg yolk based diluents for cryopreservation of goat semen.
abstract: Soybean lecithin is a suitable plant-based cryoprotectant for freezing ruminant sperm. Optimum level of lecithin was not clear for goat semen cryopreservation. The objective of this study was to investigate the effects of different levels of soybean lecithin in semen extender on post-thaw sperm quality including CASA-motion parameters, viability, plasma membrane integrity and lipid peroxidation. Semen samples were collected from 4 Mahabadi bucks using an artificial vagina. Different concentrations of soy lecithin (SL, 0.5%, 1%, 1.5%, 2% and 2.5% w/v) were compared to 15% (v/v) egg yolk-based extender (TR-EY). No significant difference was observed for sperm progressive motility, viability or plasma membrane integrity in 1.5% SL media (33.8%, 66%, and 62.7%, respectively) and TR-EY medium (35.4%, 67.2%, and 64.9%, respectively). Sperm motion characteristics (VAP, VSL, VCL, ALH and LIN) and rapid spermatozoa were improved with extender containing 1% and 1.5% SL, compared to TR-EY extender. Furthermore, egg yolk produced significantly higher malondialdehyde (4.02±0.21) than other groups. Results suggest that the optimal lecithin concentration in the semen extender was 1.5% and also soy lecithin can substitute for egg yolk during cryopreservation for caprine sperm.
authors.affiliation: Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran; Department of Physiology, School of Medicine, Mashhad University of Medical Sciences (MUMS), Mashhad, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Salmani
authors.affiliation: Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran. Electronic address: atowhidi@ut.ac.ir.
authors.forename: Armin
authors.initials: A
authors.lastname: Towhidi
authors.affiliation: Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Zhandi
authors.affiliation: Animal Breeding Center of Iran, Karaj, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Bahreini
authors.affiliation: Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran; Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Sharafi
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods* / Q000662:veterinary; D003451:Cryoprotective Agents / Q000737:chemistry* / Q000494:pharmacology; D004530:Egg Yolk; D006041:Goats; D054709:Lecithins / Q000494:pharmacology*; D008297:Male; D012662:Semen Preservation / Q000379:methods* / Q000662:veterinary; D013025:Glycine max / Q000737:chemistry*; D013081:Sperm Motility / Q000187:drug effects
chemical_list: D003451:Cryoprotective Agents; D054709:Lecithins
keywords: Caprine sperm; Cryoprotectant; Soybean lecithin