title: Highly selective hydrolysis for the outer glucose at the C-20 position in ginsenosides by β-glucosidase from Thermus thermophilus and its application to the production of ginsenoside F2 from gypenoside XVII.
abstract: β-Glucosidase from Thermus thermophilus has specific hydrolytic activity for the outer glucose at the C-20 position in protopanaxadiol-type ginsenosides without hydrolysis of the inner glucose. The hydrolytic activity of the enzyme for gypenoside XVII was optimal at pH 6.5 and 90 °C, with a half-life of 1 h with 3 g enzyme l(-1) and 4 g gypenoside XVII l(-1). Under the optimized conditions, the enzyme converted the substrate gypenoside XVII to ginsenoside F2 with a molar yield of 100 % and a productivity of 4 g l(-1) h(-1). The conversion yield and productivity of ginsenoside F2 are the highest reported thus far among enzymatic transformations.
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Republic of Korea.
authors.forename: Kyung-Chul
authors.initials: KC
authors.lastname: Shin
authors.forename: Min-Ju
authors.initials: MJ
authors.lastname: Seo
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Oh
authors.forename: Deok-Kun
authors.initials: DK
authors.lastname: Oh
mesh_terms: D004795:Enzyme Stability; D036145:Ginsenosides / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D029774:Gynostemma / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D010936:Plant Extracts / Q000378:metabolism; D013696:Temperature; D016963:Thermus thermophilus / Q000201:enzymology*; D001617:beta-Glucosidase / Q000737:chemistry / Q000378:metabolism*
chemical_list: D036145:Ginsenosides; D010936:Plant Extracts; C407883:ginsenoside F2; C474918:gypenoside; D001617:beta-Glucosidase; D005947:Glucose