title: The role of noncoding regions of classical swine fever virus C-strain in its adaptation to the rabbit.
abstract: Classical swine fever (CSF) is a devastating disease of swine caused by classical swine fever virus (CSFV). C-strain, a modified live vaccine against CSF, was developed through hundreds of passages of a highly virulent CSFV in the rabbit in China in the mid-1950s. To identify the role of noncoding regions (NCRs) of C-strain in its adaptation to the rabbit, we generated and evaluated a series of chimeric viruses derived from C-strain and the highly virulent Shimen strain. The results demonstrated that the NCRs of the C-strain were essential for its fever induction in rabbits and the coding region but not NCRs was necessary for its replication in the spleen of rabbits.
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors.forename: Junhua
authors.initials: J
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors.forename: Yuzi
authors.initials: Y
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors.forename: Bibo
authors.initials: B
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150001, China. Electronic address: huajiqiu@hvri.ac.cn.
authors.forename: Hua-Ji
authors.initials: HJ
authors.lastname: Qiu
mesh_terms: D000220:Adaptation, Biological; D000818:Animals; D002681:China; D006692:Classical Swine Fever Virus / Q000235:genetics* / Q000502:physiology*; D022661:RNA, Untranslated / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D011817:Rabbits; D011995:Recombination, Genetic; D012692:Serial Passage; D013154:Spleen / Q000821:virology; D014779:Virus Replication
chemical_list: D022661:RNA, Untranslated; D012367:RNA, Viral
keywords: Adaptation to the rabbit; C-strain; Classical swine fever virus; Fever induction; Noncoding regions; Replication in the spleen