title: Functional and structural study comparing the C-terminal amidated β-neurotoxin Ts1 with its isoform Ts1-G isolated from Tityus serrulatus venom.
abstract: Mature Ts1, the main neurotoxin from Tityus serrulatus venom, has its C-terminal Cys amidated, while the isolated isoform of Ts1, named Ts1-G, keeps the non-amidated Gly residue at the C-terminal region, allowing the study of the comparative functional importance of amidation at the C-terminal between these two native toxins. Voltage dependent sodium current measurements showed that the affinity of Ts1-G for sodium channels is smaller than that of the mature Ts1, confirming the important role played by the C-terminal amidation in determining Ts1 activity.
authors.affiliation: Department of Physics and Chemistry, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Av. do Café, s/n, 14040-903 Ribeirão Preto, SP, Brazil.
authors.forename: V A
authors.initials: VA
authors.lastname: Coelho
authors.affiliation: Department of Physics and Chemistry, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Av. do Café, s/n, 14040-903 Ribeirão Preto, SP, Brazil.
authors.forename: C M
authors.initials: CM
authors.lastname: Cremonez
authors.affiliation: Department of Physics and Chemistry, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Av. do Café, s/n, 14040-903 Ribeirão Preto, SP, Brazil.
authors.forename: F A P
authors.initials: FA
authors.lastname: Anjolette
authors.affiliation: Department of Physiology, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes, 3900 14049-900 Ribeirão Preto, SP, Brazil.
authors.forename: J F
authors.initials: JF
authors.lastname: Aguiar
authors.affiliation: Department of Physiology, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes, 3900 14049-900 Ribeirão Preto, SP, Brazil.
authors.forename: W A
authors.initials: WA
authors.lastname: Varanda
authors.affiliation: Department of Physics and Chemistry, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Av. do Café, s/n, 14040-903 Ribeirão Preto, SP, Brazil. Electronic address: ecabraga@fcfrp.usp.br.
authors.forename: E C
authors.initials: EC
authors.lastname: Arantes
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D001786:Blood Glucose / Q000187:drug effects; D005591:Chemical Fractionation; D019476:Insect Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D008297:Male; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D020033:Protein Isoforms / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity; D012604:Scorpion Venoms / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D012605:Scorpions; D016415:Sequence Alignment
chemical_list: D060829:Arthropod Proteins; D001786:Blood Glucose; D019476:Insect Proteins; D009498:Neurotoxins; D020033:Protein Isoforms; D012604:Scorpion Venoms; C120263:scorpion toxin Ts1, Tityus
keywords: C-terminal amidation; Tityus serrulatus venom; Ts1 isoform; Voltage gated sodium channels; β-Neurotoxin