title: Recent discoveries and applications involving small-molecule microarrays.
abstract: High-throughput and unbiased binding assays have proven useful in probe discovery for a myriad of biomolecules, including targets of unknown structure or function and historically challenging target classes. Over the past decade, a number of novel formats for executing large-scale binding assays have been developed and used successfully in probe discovery campaigns. Here we review the use of one such format, the small-molecule microarray (SMM), as a tool for discovering protein-small molecule interactions. This review will briefly highlight selected recent probe discoveries using SMMs as well as novel uses of SMMs in profiling applications.
authors.affiliation: Division of Hematology/Oncology, Boston Children's Hospital, Boston, MA, USA; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
authors.forename: Jiyoung A
authors.initials: JA
authors.lastname: Hong
authors.affiliation: Broad Institute of MIT and Harvard, Cambridge, MA, USA.
authors.forename: Dylan V
authors.initials: DV
authors.lastname: Neel
authors.affiliation: Broad Institute of MIT and Harvard, Cambridge, MA, USA.
authors.forename: Dina
authors.initials: D
authors.lastname: Wassaf
authors.affiliation: Broad Institute of MIT and Harvard, Cambridge, MA, USA.
authors.forename: Francisco
authors.initials: F
authors.lastname: Caballero
authors.affiliation: Broad Institute of MIT and Harvard, Cambridge, MA, USA; David H. Koch Institute for Integrative Cancer Research, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA. Electronic address: koehler@mit.edu.
authors.forename: Angela N
authors.initials: AN
authors.lastname: Koehler
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002470:Cell Survival; D006801:Humans; D046228:Microarray Analysis / Q000379:methods*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D054852:Small Molecule Libraries / Q000032:analysis* / Q000378:metabolism
chemical_list: D015415:Biomarkers; D011506:Proteins; D054852:Small Molecule Libraries