title: Response surface methodology for the optimization of beta-lactoglobulin nano-liposomes.
abstract: Response surface methodology based on a central composite rotatable design has been successfully used to model and optimize biochemical and biotechnological processes. Heat treatment could have an effect on beta-lactoglobulin (β-Lg) and lead to allergic reaction. To reduce this phenomenon, liposomes were used as carriers in this research. The mass ratio of phosphatidylcholine and cholesterol (2.5-12.5), β-Lg concentration (2.5-12.5 mg mL(-1)), sonication time (5-25, min) and temperature (25-40 °C) were selected as independent variables with encapsulation efficiency as a dependent variable. For each response, a second-order polynomial model was developed using multiple linear regression analysis. Applying a desirability function method the optimum parameters were: phosphatidylcholine to cholesterol ratio of 8.05, β-Lg concentration of 9.09 mg mL(-1), sonication time of 17.71 min and temperature of 30 °C. The particle size and encapsulation efficiency were found to be 189 nm and 61.55%, respectively. Furthermore, the in vitro stability of β-Lg nano-liposomes in simulated gastrointestinal juice was evaluated. The nano-liposomes showed an acceptable stability in simulated gastrointestinal juice at 37 °C for 4 h.
authors.affiliation: Hangzhou Test Institute of Quality, Hangzhou, Zhejiang 310019, China.
authors.forename: Jieqing
authors.initials: J
authors.lastname: Ma
authors.forename: Rongfa
authors.initials: R
authors.lastname: Guan
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Chen
authors.forename: Yanbo
authors.initials: Y
authors.lastname: Wang
authors.forename: Yunbin
authors.initials: Y
authors.lastname: Hao
authors.forename: Xingqian
authors.initials: X
authors.lastname: Ye
authors.forename: Mingqi
authors.initials: M
authors.lastname: Liu
mesh_terms: D002784:Cholesterol / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D004339:Drug Compounding / Q000379:methods*; D007782:Lactoglobulins / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010713:Phosphatidylcholines / Q000737:chemistry
chemical_list: D004337:Drug Carriers; D007782:Lactoglobulins; D008081:Liposomes; D010713:Phosphatidylcholines; D002784:Cholesterol