title: Application of mixture analysis to crude materials from natural resources (IV)[1(a-c)]: identification of Glycyrrhiza species by direct analysis in real time mass spectrometry (II).
abstract: In order to identify Glycyrrhiza species by chemical fingerprinting, the bark of the roots and stolons of Glycyrrhiza uralensis Fischer and G. glabra Linné were analyzed using DART (Direct Analysis in Real Time)-MS. The characteristic peaks of each species were determined statistically by volcano plot. This summarizes the relationship between the p-values of a statistical test and the magnitude of the difference in values of the samples in the groups. In this experiment, peaks that had a p value <0.05 in the t test and Z2 absolute difference were defined as characteristic. As a result, characteristic peaks of G. uralensis were found at m/z 299, 315, 341, and 369. In contrast, characteristic peaks of G. glabra were found at m/z 323, 325, 337, 339, and 391. In conclusion, we found several characteristic peaks to distinguish G. uralensis and G. glabra by DART-MS using volcano plot. This method can be applied to identify the Glycyrrhiza species.
authors.affiliation: Department of Natural Medicine and Phytochemistry, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan.
authors.forename: Eriko
authors.initials: E
authors.lastname: Fukuda
authors.affiliation: Department of Clinical Pharmaceutics, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Uesawa
authors.affiliation: Department of Natural Medicine and Phytochemistry, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Baba
authors.affiliation: Department of Natural Medicine and Phytochemistry, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan.
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Okada
mesh_terms: D029882:Glycyrrhiza uralensis / Q000737:chemistry* / Q000145:classification; D013058:Mass Spectrometry; D024301:Plant Bark / Q000737:chemistry; D013045:Species Specificity