title: [Exploration of toxicity reducing mechanism of aconite alkaloids during decoction process using liquid chromatography-mass spectrometry].
abstract: A high performance liquid chromatography-electrospray ionization mass spectrometry method was developed for the determination of aconite alkaloids. It was used to investigate the degradation of alkaloids of Radix Aconiti Lateralis Preparata during decoction. Six alkaline degradation products were identified, and the degradation regularity of diester-diterpenoid alkaloids was confirmed during the test using standards. The dynamic changes of the amount of aconite alkaloids in the decoction of Radix Aconiti Lateralis Preparata were supervised. Along with the increase of decoction time, the concentrations of diester-diterpenoid alkaloids and lipo-alkaloid decreased significantly. The results can provide a scientific basis for the safety use of aconite.
authors.affiliation: Zhejiang Provincial Hospital of Traditional Chinese Medicine, Hangzhou 310006, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.affiliation: Zhejiang Provincial Hospital of Traditional Chinese Medicine, Hangzhou 310006, China.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Chen
authors.affiliation: Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Tong
authors.affiliation: Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Xu
mesh_terms: D000157:Aconitine / Q000737:chemistry*; D031841:Aconitum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D013058:Mass Spectrometry
chemical_list: D004365:Drugs, Chinese Herbal; D000157:Aconitine