title: Production of a monoclonal antibody for C/EBPβ: the subnuclear localization of C/EBPβ in mouse L929 cells.
abstract: The CCAAT/enhancer-binding protein (C/EBP)β belongs to the C/EBP family of proteins that possesses a basic leucine zipper DNA-binding domain. These proteins bind DNA by dimerization and play a role in the transcriptional regulation of various cells. There are six different types of C/EBPs, and some form isoforms through the use of alternative translation initiation sites. The functional analysis of the C/EBP family is therefore difficult to achieve. Here we report on the production of specific monoclonal antibodies against mouse C/EBPβ using a rat medial iliac lymph node method. Immunoblotting using C/EBPβ monoclonal antibodies identified two types of isoforms, while immunostaining revealed a subnuclear localization for C/EBPβ. Use of this antibody should contribute to the further elucidation of the transcriptional regulatory function of C/EBPβ.
authors.affiliation: 1 Department of Advanced Medical Initiatives, JST-CREST, Faculty of Medicine, Kyushu University , Fukuoka, Japan .
authors.forename: Akihito
authors.initials: A
authors.lastname: Harada
authors.forename: Etsuko
authors.initials: E
authors.lastname: Okazaki
authors.forename: Seiji
authors.initials: S
authors.lastname: Okada
authors.forename: Taro
authors.initials: T
authors.lastname: Tachibana
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Ohkawa
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology* / Q000493:pharmacokinetics*; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000276:immunology*; D002460:Cell Line; D005260:Female; D006825:Hybridomas / Q000276:immunology; D015151:Immunoblotting; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D051379:Mice; D020033:Protein Isoforms / Q000235:genetics / Q000276:immunology; D051381:Rats; D011921:Rats, Inbred WKY; D014158:Transcription, Genetic
chemical_list: D000911:Antibodies, Monoclonal; D022782:CCAAT-Enhancer-Binding Protein-beta; D020033:Protein Isoforms