title: Semiquinone glucoside derivative (SQGD) isolated from Bacillus sp. INM-1 protects against gamma radiation-induced oxidative stress.
abstract: In the present study, radioprotective potential of Semiquinone glucoside derivative (SQGD) isolated from radioresistant bacterium Bacillus sp. INM-1 was evaluated. γ-Radiation induced protein carbonylation, plasmid DNA damage, enzyme functional impairment, lipid peroxidation, HO radicals generation and their protection by SQGD was assessed. As a result of SQGD treatment, significant inhibition (p<0.05) in protein carbonylation was observed with BSA. SQGD treatment was found to restore supercoiled (~70±3.21%) form of irradiated plasmid DNA against γ-irradiation. SQGD protects enzymes (EcoR1 and BamH1) against radiation-induced dysfunctioning. SQGD significantly inhibited (p<0.05) lipid peroxidation in liposomes, brain and liver homogenate. Higher HO(•) radicals-averting activity of SQGD was observed in the serum and liver homogenate of C57BL/6 mice against H2O2-induced oxidative stress. In conclusion, SQGD demonstrates excellent radical-scavenging activity towards bio-macromolecules in irradiated environment and can be developed as an ideal radioprotector against radiation-induced oxidative stress in future.
authors.affiliation: Radiation Biotechnology Laboratory, Department of Radiation Biosciences, Institute of Nuclear and Allied Sciences, Delhi 110054, India.
authors.forename: Saurabh
authors.initials: S
authors.lastname: Mishra
authors.affiliation: Radiation Biotechnology Laboratory, Department of Radiation Biosciences, Institute of Nuclear and Allied Sciences, Delhi 110054, India.
authors.forename: Poonam
authors.initials: P
authors.lastname: Malhotra
authors.affiliation: Radiation Biotechnology Laboratory, Department of Radiation Biosciences, Institute of Nuclear and Allied Sciences, Delhi 110054, India.
authors.forename: Ashutosh K
authors.initials: AK
authors.lastname: Gupta
authors.affiliation: Radiation Biotechnology Laboratory, Department of Radiation Biosciences, Institute of Nuclear and Allied Sciences, Delhi 110054, India.
authors.forename: Praveen K
authors.initials: PK
authors.lastname: Singh
authors.affiliation: Department of Biochemistry, Faculty of Science, Jamia Hamdard, Delhi 110062, India.
authors.forename: Saleem
authors.initials: S
authors.lastname: Javed
authors.affiliation: Radiation Biotechnology Laboratory, Department of Radiation Biosciences, Institute of Nuclear and Allied Sciences, Delhi 110054, India; Department of Biochemistry, Faculty of Science, Jamia Hamdard, Delhi 110062, India. Electronic address: rajkumar790@yahoo.com.
authors.forename: Raj
authors.initials: R
authors.lastname: Kumar
mesh_terms: D000818:Animals; D001407:Bacillus; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects; D005720:Gamma Rays / Q000009:adverse effects*; D005960:Glucosides / Q000494:pharmacology*; D017665:Hydroxyl Radical / Q000378:metabolism; D007422:Intestines; D008081:Liposomes; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000187:drug effects; D011809:Quinones / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D005960:Glucosides; D008081:Liposomes; D011809:Quinones; D011837:Radiation-Protective Agents; D017392:Thiobarbituric Acid Reactive Substances; D017665:Hydroxyl Radical; D008315:Malondialdehyde
keywords: Antioxidant; Carbonylation; Lipid-peroxidation; Oxidative stress; Restriction enzyme; Toxicity