title: Guanine-tethered antisense oligonucleotides as synthetic riboregulators.
abstract: Regulation of gene expression by short oligonucleotides (antisense oligonucleotides), which can modulate RNA structures and inhibit subsequent associations with the translation machinery, is a potential approach for gene therapy. This chapter describes an alternative antisense strategy using guanine-tethered antisense oligonucleotides (G-ASs) to introduce a DNA-RNA heteroquadruplex structure at a designated sequence on RNA targets. The feasibility of using G-ASs to modulate RNA conformation may allow control of RNA function by inducing biologically important quadruplex structures. This approach to manipulate quadruplex structures using G-ASs may expand the strategies for regulating RNA structures and the functions of short oligonucleotide riboregulators.
authors.affiliation: Graduate School of Science and Technology, Hirosaki University, Hirosaki, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Hagihara
mesh_terms: D002942:Circular Dichroism; D054856:G-Quadruplexes; D005786:Gene Expression Regulation; D006147:Guanine / Q000031:analogs & derivatives*; D009690:Nucleic Acid Conformation; D016376:Oligonucleotides, Antisense / Q000737:chemistry* / Q000378:metabolism*; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet
chemical_list: D016376:Oligonucleotides, Antisense; D006147:Guanine; D012313:RNA