title: Search for peripheral biomarkers in patients affected by acutely psychotic bipolar disorder: a proteomic approach.
abstract: Data on neurobiological mechanisms underlying mood disorders are elusive; the aetiology of such states is multifactorial, including genetic predisposition and environmental factors. Diagnosis is currently being made only on an interview-based methodology. Biological markers, which could improve the current classification, and in perspective, stratify patients on a biological basis into more homogeneous clinically distinct subgroups, are highly needed. We describe here a comparative proteomic analysis of peripheral lymphocytes from patients affected by acute psychotic bipolar disorder (PBD) (n = 15), major depressive episode (MDE) with no personal or family history of psychosis (n = 11), and a group of demographically matched healthy controls (HC) (n = 15). All patients were evaluated by means of Structured Clinical Interview for DSM-IV-Patient version (SCID-I-P), Positive and Negative Symptoms Scale (PANSS), Young Mania Rating Scale (YMRS), Hamilton Anxiety Rating Scale (HAM-A) and Hamilton Depression Rating Scale (HAM-D-17) questionnaires. Blood lymphocytes were obtained by gradient separation, and 2-DE was carried out on protein extracts. Significant differences in protein patterns among the three groups were observed. Thirty-six protein spots were found to be differentially expressed in patients compared to controls, which collapsed into 25 different proteins after mass spectrometry identification. Twenty-one of these proteins failed to discriminate between PBD and MDE, suggesting common signatures for these disorders. Nevertheless, after the western blot validation only two of the remaining proteins, namely LIM and SH3 domain protein1, and short-chain specific acyl-CoA dehydrogenase mitochondrial protein, resulted in being significantly upregulated in PBD samples suggesting additional mechanisms that could be associated with the psychotic features of bipolar disorder.
authors.affiliation: Department of Pharmacy, University of Pisa, Via Bonanno 6, 56126 Pisa, Italy. lucas@farm.unipi.it.
authors.forename: Laura
authors.initials: L
authors.lastname: Giusti
authors.forename: Valentina
authors.initials: V
authors.lastname: Mantua
authors.forename: Ylenia
authors.initials: Y
authors.lastname: Da Valle
authors.forename: Federica
authors.initials: F
authors.lastname: Ciregia
authors.forename: Tiziana
authors.initials: T
authors.lastname: Ventroni
authors.forename: Ginevra
authors.initials: G
authors.lastname: Orsolini
authors.forename: Elena
authors.initials: E
authors.lastname: Donadio
authors.forename: Gino
authors.initials: G
authors.lastname: Giannaccini
authors.forename: Mauro
authors.initials: M
authors.lastname: Mauri
authors.forename: Giovanni Battista
authors.initials: GB
authors.lastname: Cassano
authors.forename: Antonio
authors.initials: A
authors.lastname: Lucacchini
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D000328:Adult; D015415:Biomarkers / Q000097:blood; D001714:Bipolar Disorder / Q000097:blood* / Q000378:metabolism / Q000473:pathology*; D044926:Butyryl-CoA Dehydrogenase / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D003598:Cytoskeletal Proteins / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D003865:Major Depressive Disorder / Q000097:blood* / Q000378:metabolism / Q000473:pathology*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D060588:LIM Domain Proteins / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D040901:Proteomics
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D015415:Biomarkers; D003598:Cytoskeletal Proteins; C096353:LASP1 protein, human; D060588:LIM Domain Proteins; D044926:Butyryl-CoA Dehydrogenase