title: Neolignans as xanthine oxidase inhibitors from Hyptis rhomboides.
abstract: The active fraction from the EtOH extract of Hyptis rhomboides against xanthine oxidase was identified by use of an HPLC microfractionation-centrifugal vacuum evaporation-bioassay hyphenated technique. Scale-up separation of the active subfractions using semi-preparative RP-HPLC provided 13 phenylpropanoid compounds, including O-styrenylneolignans, hyprhombins A-C, epihyprhombin B, and hyprhombin B methyl ester, and O-caffeoylnorneolignans, hyprhombins D and E. All of these compounds shared a common 1,4-benzodioxane skeleton, as established by spectroscopic analyses. Hyprhombin C and epihyprhombin B exhibited better anti-xanthine oxidase activity than allopurinol, with IC50 values of 0.6 and 2.0 μM, respectively.
authors.affiliation: School of Pharmacy, College of Medicine, National Taiwan University, 1, Sec. 1, Jen-Ai Rd, Taipei 10051, Taiwan, Republic of China.
authors.forename: Sheng-Fa
authors.initials: SF
authors.lastname: Tsai
authors.affiliation: School of Pharmacy, College of Medicine, National Taiwan University, 1, Sec. 1, Jen-Ai Rd, Taipei 10051, Taiwan, Republic of China. Electronic address: shoeilee@ntu.edu.tw.
authors.forename: Shoei-Sheng
authors.initials: SS
authors.lastname: Lee
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D031331:Hyptis / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D017705:Lignans / Q000302:isolation & purification / Q000494:pharmacology*; D015394:Molecular Structure; D035261:Plant Components, Aerial / Q000737:chemistry; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors*
chemical_list: D004365:Drugs, Chinese Herbal; D004791:Enzyme Inhibitors; D017705:Lignans; D014969:Xanthine Oxidase
keywords: 1,4-Benzodioxane; HPLC microfractionation–centrifugal vacuum evaporation–bioassay hyphenation; Hyprhombins; Hyptis rhomboides; Labiatae; Neolignans; Xanthine oxidase inhibitor