title: Sustained in vivo depletion of splenic langerin(+) CD8α(+) dendritic cells is well-tolerated by lang-DTREGFP mice.
abstract: Splenic langerin(+) CD8α(+) dendritic cells (DCs) have exhibited a critical role in cross-priming CD8(+) T cell responses. To further study the roles of this DC subset, a protocol for the continuous depletion of langerin(+) CD8α(+) DCs was established using the pre-existing lang-DTREGFP mouse model. Due to the fast turnover rate of splenic CD8α(+) DCs, maintaining the depletion of langerin(+) CD8α(+) DCs required multiple diphtheria toxin (DT) treatments. We found that prolonged treatment with DT did not cause weight loss, or neutrophilia, as reported in some DT-based depletion models. Therefore, the in vivo depletion of murine langerin(+) CD8α(+) DCs can be maintained over time to analyse their function during the full course of an immune response.
authors.affiliation: Malaghan Institute of Medical Research, Wellington, New Zealand; School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand.
authors.forename: Kelly A
authors.initials: KA
authors.lastname: Prendergast
authors.affiliation: Malaghan Institute of Medical Research, Wellington, New Zealand; School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand.
authors.forename: Taryn L
authors.initials: TL
authors.lastname: Osmond
authors.affiliation: Malaghan Institute of Medical Research, Wellington, New Zealand; Department of Pathology and Molecular Medicine, University of Otago, Wellington, New Zealand.
authors.forename: Sotaro
authors.initials: S
authors.lastname: Ochiai
authors.affiliation: Malaghan Institute of Medical Research, Wellington, New Zealand; School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand.
authors.forename: Ian F
authors.initials: IF
authors.lastname: Hermans
authors.affiliation: Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand. Electronic address: jo.kirman@otago.ac.nz.
authors.forename: Joanna R
authors.initials: JR
authors.lastname: Kirman
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D016827:CD8 Antigens / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D004167:Diphtheria Toxin / Q000008:administration & dosage / Q000276:immunology*; D037181:Lectins, C-Type / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D008212:Lymphocyte Depletion; D037241:Mannose-Binding Lectins / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D013154:Spleen / Q000166:cytology / Q000276:immunology
chemical_list: D000954:Antigens, Surface; D016827:CD8 Antigens; C431698:CD8alpha antigen; C498497:Cd207 protein, mouse; D004167:Diphtheria Toxin; D037181:Lectins, C-Type; D037241:Mannose-Binding Lectins
keywords: Dendritic cells; Depletion; Diphtheria toxin; Langerin