title: Biochemical markers of pollutant responses in macrozoobenthos from the White Sea: intracellular proteolysis.
abstract: Coastal environments of Kandalaksha Gulf in the White Sea (Russia) despite nature conservation efforts are heavily influenced by human activities. Biological effects of complex environmental pollution, including organic substances, heavy metals, and oil hydrocarbons, were assessed in widely distributed marine invertebrates, Gammarus duebeni (Crustacea, Amphipoda) and Mytilus edulis (Mollusca, Bivalvia), collected from a series of anthropogenically-impacted areas and distanced reference sites in Kandalaksha Gulf. The parameters of intracellular protein degradation pathways such as cytosol calpain system and lysosomal cathepsins B (CatB) and cathepsin D (CatD) were studied. The response reactions observed in invertebrates vary in specificity and ranged from adaptive to destructive depending on the total contaminant level and the nature of predominant pollutant. The ecological relevance of studied parameters as biomarkers was confirmed by their ability to indicate both expose to pollutants and adverse effects at the organism level.
authors.affiliation: Laboratory of Environmental Biochemistry, Institute of Biology, Karelian Research Centre of Russian Academy of Science, 185910 Petrozavodsk, Pushkinskaya str., 11, Russian Federation. Electronic address: l-lysenko@yandex.ru.
authors.forename: Liudmila
authors.initials: L
authors.lastname: Lysenko
authors.affiliation: Laboratory of Environmental Biochemistry, Institute of Biology, Karelian Research Centre of Russian Academy of Science, 185910 Petrozavodsk, Pushkinskaya str., 11, Russian Federation.
authors.forename: Nadezda
authors.initials: N
authors.lastname: Kantserova
authors.affiliation: Laboratory of Environmental Biochemistry, Institute of Biology, Karelian Research Centre of Russian Academy of Science, 185910 Petrozavodsk, Pushkinskaya str., 11, Russian Federation.
authors.forename: Elena
authors.initials: E
authors.lastname: Käiväräinen
authors.affiliation: Laboratory of Environmental Biochemistry, Institute of Biology, Karelian Research Centre of Russian Academy of Science, 185910 Petrozavodsk, Pushkinskaya str., 11, Russian Federation.
authors.forename: Marina
authors.initials: M
authors.lastname: Krupnova
authors.affiliation: Petrozavodsk State University, 185000 Petrozavodsk, Lenina pr., 33, Russian Federation.
authors.forename: Galina
authors.initials: G
authors.lastname: Shklyarevich
authors.affiliation: Laboratory of Environmental Biochemistry, Institute of Biology, Karelian Research Centre of Russian Academy of Science, 185910 Petrozavodsk, Pushkinskaya str., 11, Russian Federation.
authors.forename: Nina
authors.initials: N
authors.lastname: Nemova
mesh_terms: D033304:Amphipoda / Q000187:drug effects*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D002850:Chromatography, Gel; D004781:Environmental Exposure; D004784:Environmental Monitoring; D049880:Mytilus edulis / Q000187:drug effects*; D009792:Oceans and Seas; D010447:Peptide Hydrolases / Q000378:metabolism*; D059748:Proteolysis / Q000187:drug effects; D012426:Russia; D013045:Species Specificity; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D014874:Water Pollutants, Chemical; D010447:Peptide Hydrolases
keywords: Amphipod; Biochemistry; Coastal zone; Intracellular protease; Mussel; Pollution effects; White Sea