title: Mesenchymal stem cells with modification of junctional adhesion molecule a induce hair formation.
abstract: The junctional adhesion molecule A (JAM-A) has been shown to serve a crucial role in the proliferation, differentiation, and tube-like formation of epithelial cells during angiogenesis. The role of JAM-A in hair follicle (HF) regeneration has not yet been reported. In this study, we used human JAM-A-modified human mesenchymal stem cells (MSCs) to repair HF abnormalities in BALB/c nu/nu mice. The JAM-A gene and JAM-A short hairpin RNA were transfected into cultured human MSCs to generate the JAM-A overexpression MSCs (JAM-A(ov) MSCs) and JAM-A knockdown MSCs (JAM-A(kd) MSCs), respectively. These cells were injected intradermally into the skin of nude mice during the first telogen phase of the HF that occurs 21 days postnatally. We found that JAM-A(ov) MSCs migrated into the HF sheath and remodeled HF structure effectively. The HF abnormalities such as HF curve and HF zigzag were remodeled, and hair formation was improved 7 days following injection in both the JAM-A(ov) MSC and MSC groups, compared with the JAM-A(kd) MSC group or negative control group. Furthermore, the JAM-A(ov) MSC group showed enhanced hair formation in contrast to the MSC group, and the number of curved and zigzagged HFs was reduced by 80% (p < .05). These results indicated that JAM-A(ov) MSCs improved hair formation in nude mice through HF structure remodeling.
authors.affiliation: Research Center of Developmental Biology, Department of Histology and Embryology, and Department of Mathematics, Second Military Medical University, Shanghai, People's Republic of China.
authors.forename: Minjuan
authors.initials: M
authors.lastname: Wu
authors.forename: Xiaocan
authors.initials: X
authors.lastname: Guo
authors.forename: Ling
authors.initials: L
authors.lastname: Yang
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Tang
authors.forename: Yongji
authors.initials: Y
authors.lastname: Yang
authors.forename: Houqi
authors.initials: H
authors.lastname: Liu
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000235:genetics; D002454:Cell Differentiation; D002465:Cell Movement; D018920:Coculture Techniques; D055785:Gene Knockdown Techniques; D018859:Hair Follicle / Q000166:cytology / Q000378:metabolism*; D064593:Heterografts; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000235:genetics; D013997:Time Factors
chemical_list: D015815:Cell Adhesion Molecules; C407243:F11R protein, human; D011956:Receptors, Cell Surface
keywords: Cell adhesion molecules; Cell transplantation; Differentiation; Mesenchymal stem cells