title: The development of transgenic mice for the expression of large amounts of human lysozyme in milk.
abstract: Human lysozyme (hLYZ) has important potential applications as antimicrobial medicine and food additive. To develop a robust expression vector that ensures expression of large amounts of hLYZ in milk, here a 26,267 bp chimeric mouse whey acidic protein (mWAP)::hLYZ cassette was constructed and used as a mammary gland-specific expression vector, in which a 3,010 bp genomic sequence in the 24,466 bp mWAP gene locus was substituted by a 4,811 bp genomic sequence of hLYZ, exactly from the start codon to the stop codon. Corresponding transgenic mice were generated, and enzymatically-active hLYZ was expressed at 18.4-35 g l(-1) in the milk of most transgenic mouse lines. Our transgenic mice carrying chimeric mWAP::hLYZ represent a model system for cost-effective production of hLYZ.
authors.affiliation: Cell Engineering Department, Beijing Institute of Biotechnology, No. 20 Dongdajie Street, Beijing, 100071, People's Republic of China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Wu
authors.forename: Yanli
authors.initials: Y
authors.lastname: Lin
authors.forename: Yongyi
authors.initials: Y
authors.lastname: Xi
authors.forename: Zhenlu
authors.initials: Z
authors.lastname: Shao
authors.forename: Yanrong
authors.initials: Y
authors.lastname: Zhou
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Hongxing
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D008894:Milk Proteins / Q000096:biosynthesis* / Q000235:genetics; D008895:Milk, Human / Q000737:chemistry*; D009113:Muramidase / Q000096:biosynthesis* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D008894:Milk Proteins; D011993:Recombinant Fusion Proteins; C024471:whey acidic proteins; D009113:Muramidase