title: Development- and cold-regulated accumulation of cold shock domain proteins in wheat.
abstract: Cold shock domain (CSD) proteins, or Y-box proteins, are nucleic acid-binding proteins that are widely distributed from bacteria to higher plants and animals. Bacterial CSD proteins play an essential role in cold adaptation by destabilizing RNA secondary structures. WHEAT COLD SHOCK DOMAIN PROTEIN 1 (WCSP1) shares biochemical functions with bacterial CSD proteins and is possibly involved in cold adaptation. In this study, the temporal and spatial distribution of the wheat cold shock domain protein family (WCSPs) was serologically characterized with regard to plant development and cold adaptation. Four WCSP genes were identified through database analysis and were classified into three classes based on their molecular masses and protein domain structures. Class I (20 kD) and class II (23 kD) WCSPs demonstrated a clear pattern of accumulation in root and shoot meristematic tissues during vegetative growth. In response to cold, marked increases in WCSP levels were observed but the pattern of accumulation differed by tissue. Accumulation of WCSPs in crown tissue during cold acclimation was observed in the winter cultivar 'Chihokukomugi' but not in the spring cultivar 'Haruyutaka', suggesting a possible function for WCSPs in cold acclimation. During flower and seed development, protein levels of class I and class II WCSPs remained high. The class III WCSP (27 kD) was detected only during seed development. The highest level of class III WCSP accumulation was observed at the milky seed stage. Together, the results of this study provide a view of CSD protein accumulation throughout the life cycle of wheat and suggest that WCSPs function differentially in plant development and cold adaptation.
authors.affiliation: Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization (NARO), Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan; AgroBioInstitute, 8 Dragan Tzankov Bvld., Sofia 1000, Bulgaria.
authors.forename: Mariana
authors.initials: M
authors.lastname: Radkova
authors.affiliation: Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization (NARO), Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan; Department of Genetics and Plant Breeding, Crop Research Institute, Drnovská 507, 161 06 Prague 6, Ruzyně, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Vítámvás
authors.affiliation: Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization (NARO), Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Sasaki
authors.affiliation: Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization (NARO), Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan; Graduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo 060-8589, Japan. Electronic address: rzi@affrc.go.jp.
authors.forename: Ryozo
authors.initials: R
authors.lastname: Imai
mesh_terms: D000064:Acclimatization / Q000235:genetics*; D057773:Cold Shock Proteins and Peptides / Q000235:genetics / Q000378:metabolism*; D003080:Cold Temperature; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D035264:Flowers / Q000254:growth & development; D005786:Gene Expression Regulation; D017343:Genes, Plant; D018519:Meristem / Q000254:growth & development / Q000378:metabolism*; D063245:Plant Development / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D012621:Seasons; D012639:Seeds / Q000254:growth & development; D013045:Species Specificity; D014908:Triticum / Q000145:classification / Q000235:genetics / Q000378:metabolism*
chemical_list: D057773:Cold Shock Proteins and Peptides; D004268:DNA-Binding Proteins; D010940:Plant Proteins; D016601:RNA-Binding Proteins
keywords: Cold acclimation; Cold shock domain proteins; Flower development; Seed development