title: Activated peritoneal cavity B-1a cells possess regulatory B cell properties.
abstract: Previous studies have suggested that murine peritoneal cavity-derived B-1a cells possess similarities with described regulatory B cell subsets. The aim of the current study was to examine the potential immunoregulatory function of peritoneal cavity-derived B(-1a) cells. In vitro activation of peritoneal cavity-derived B- and B-1a cells shows that activation of these B cells with anti-CD40 and LPS induces these cells to secrete more IL-10, IL-6 and IgM as compared to splenic B cells. In a suppression assay, CD40/TLR4-activated peritoneal cavity B cells possess regulatory B cell functions as they inhibit the capacity of CD4(+) T cells to produce both tumor necrosis factor-α and interferon-γ. Splenic B cells did not show this, whereas non-activated peritoneal cavity B cells augmented the capacity of CD4(+) T cells to produce tumor necrosis factor-α, while the ability to produce interferon-γ was not altered. The current paper compares splenic B cells to peritoneal cavity B(-1a) cells in an in vitro activation- and an suppression-assay and concludes that peritoneal cavity B(-1a) cells possess properties that appear similar to splenic autoimmune-suppressive regulatory B cell subsets described in the literature.
authors.affiliation: Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands.
authors.forename: Bram
authors.initials: B
authors.lastname: Margry
authors.affiliation: Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands.
authors.forename: Saskia C W
authors.initials: SC
authors.lastname: Kersemakers
authors.affiliation: Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands.
authors.forename: Aad
authors.initials: A
authors.lastname: Hoek
authors.affiliation: Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands.
authors.forename: Ger J A
authors.initials: GJ
authors.lastname: Arkesteijn
authors.affiliation: Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands.
authors.forename: Willemien H
authors.initials: WH
authors.lastname: Wieland
authors.affiliation: Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands.
authors.forename: Willem
authors.initials: W
authors.lastname: van Eden
authors.affiliation: Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands.
authors.forename: Femke
authors.initials: F
authors.lastname: Broere
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D060151:B-Lymphocytes, Regulatory / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D019013:CD40 Antigens / Q000276:immunology; D018920:Coculture Techniques; D005260:Female; D007113:Immunity, Innate; D007075:Immunoglobulin M / Q000276:immunology / Q000378:metabolism; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D016753:Interleukin-10 / Q000276:immunology / Q000378:metabolism; D015850:Interleukin-6 / Q000276:immunology / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D010529:Peritoneal Cavity / Q000166:cytology*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000378:metabolism
chemical_list: D000906:Antibodies; D019013:CD40 Antigens; C556062:IL10 protein, mouse; D007075:Immunoglobulin M; D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma