title: A simple Fourier filter for suppression of the missing wedge ray artefacts in single-axis electron tomographic reconstructions.
abstract: The limited specimen tilting range that is typically available in electron tomography gives rise to a region in the Fourier space of the reconstructed object where experimental data are unavailable - the missing wedge. Since this region is sharply delimited from the area of available data, the reconstructed signal is typically hampered by convolution with its impulse response, which gives rise to the well-known missing wedge artefacts in 3D reconstructions. Despite the recent progress in the field of reconstruction and regularization techniques, the missing wedge artefacts remain untreated in most current reconstruction workflows in structural biology. Therefore we have designed a simple Fourier angular filter that effectively suppresses the ray artefacts in the single-axis tilting projection acquisition scheme, making single-axis tomographic reconstructions easier to interpret in particular at low signal-to-noise ratio in acquired projections. The proposed filter can be easily incorporated into current electron tomographic reconstruction schemes.
authors.affiliation: Charles University in Prague, First Faculty of Medicine, Institute of Cellular Biology and Pathology, Albertov 4, 128 01 Prague 2, Czech Republic. Electronic address: Lubomir.Kovacik@lf1.cuni.cz.
authors.forename: Lubomír
authors.initials: L
authors.lastname: Kováčik
authors.forename: Sami
authors.initials: S
authors.lastname: Kereïche
authors.affiliation: Charles University in Prague, First Faculty of Medicine, Institute of Cellular Biology and Pathology, Albertov 4, 128 01 Prague 2, Czech Republic.
authors.forename: Sami
authors.initials: S
authors.lastname: Kerïeche
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK; MPI-CBG, Photenhauerstr. 108, 01307 Dresden, Germany.
authors.forename: Johanna L
authors.initials: JL
authors.lastname: Höög
authors.affiliation: Charles University in Prague, First Faculty of Medicine, Institute of Cellular Biology and Pathology, Albertov 4, 128 01 Prague 2, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Jůda
authors.affiliation: Centre for Biomedical Image Analysis, Faculty of Informatics, Masaryk University, Brno, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Matula
authors.affiliation: Charles University in Prague, First Faculty of Medicine, Institute of Cellular Biology and Pathology, Albertov 4, 128 01 Prague 2, Czech Republic.
authors.forename: Ivan
authors.initials: I
authors.lastname: Raška
mesh_terms: D000818:Animals; D016477:Artifacts; D002531:Cerebellum / Q000648:ultrastructure; D031211:Corylus / Q000648:ultrastructure; D055032:Electron Microscope Tomography / Q000379:methods*; D005583:Fourier Analysis; D007091:Image Processing, Computer-Assisted; D011058:Pollen / Q000648:ultrastructure; D051381:Rats; D059629:Signal-To-Noise Ratio; D014346:Trypanosoma brucei brucei / Q000648:ultrastructure
keywords: Electron tomography; Missing wedge; Missing wedge artefacts; Single-axis tilting