title: A protein synthesis-dependent mechanism sustains calcium-permeable AMPA receptor transmission in nucleus accumbens synapses during withdrawal from cocaine self-administration.
abstract: Extended-access cocaine self-administration results in withdrawal-dependent incubation of cocaine craving. Rats evaluated after ∼1 month of withdrawal from such regimens ("incubated rats") exhibit changes in medium spiny neurons (MSNs) of the nucleus accumbens (NAc) that include accumulation of Ca(2+)-permeable AMPA receptors (CP-AMPARs) and a switch in group I metabotropic glutamate receptor (mGluR)-mediated suppression of synaptic transmission from mGluR5-dependent to mGluR1-dependent. To determine the role of protein synthesis in mediating these adaptations, we conducted whole-cell patch-clamp recordings in NAc core MSNs of "incubated rats" in the presence of translational inhibitors (anisomycin, cycloheximide, rapamycin) or the transcriptional inhibitor actinomycin-D. The contribution of CP-AMPARs to synaptic transmission was determined by the rectification index and the sensitivity to the CP-AMPAR antagonist 1-naphthyl acetyl spermine. We found that CP-AMPAR-mediated transmission in the NAc of "incubated rats" was reduced to levels comparable to those found in saline control rats when brain slices were treated with translational inhibitors, whereas actinomycin-D had no effect. We also investigated the effect of protein translation inhibitors on the switch of mGluR function in MSNs of "incubated rats" using the group I mGluR agonist (S)-3,5-dihydroxyphenylglycine in combination with either an mGluR1 (LY367385) or an mGluR5 (3-[(2-methyl-4-thiazolyl)ethynyl]pyridine) antagonist. Data revealed that inhibition of protein translation eliminated the mGluR1-mediated inhibition and restored the mGluR5 responsiveness to a state functionally similar to that of saline control rats. Together, these results suggest that aberrant regulation of local protein synthesis contributes to the maintenance of adaptations accrued at NAc MSN synapses during incubation of cocaine craving.
authors.affiliation: Department of Cellular and Molecular Pharmacology and Department of Neuroscience, The Chicago Medical School at Rosalind Franklin University of Medicine and Science, North Chicago, Illinois 60064.
authors.forename: Andrew F
authors.initials: AF
authors.lastname: Scheyer
authors.forename: Marina E
authors.initials: ME
authors.lastname: Wolf
authors.forename: Kuei Y
authors.initials: KY
authors.lastname: Tseng
mesh_terms: D000818:Animals; D001565:Benzoates / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D019970:Cocaine-Related Disorders / Q000503:physiopathology*; D003513:Cycloheximide / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005998:Glycine / Q000031:analogs & derivatives / Q000494:pharmacology; D008297:Male; D008734:Methoxyhydroxyphenylglycol / Q000031:analogs & derivatives / Q000494:pharmacology; D009714:Nucleus Accumbens / Q000502:physiology*; D018408:Patch-Clamp Techniques; D014176:Protein Biosynthesis / Q000502:physiology*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D018091:Receptors, AMPA / Q000502:physiology*; D012646:Self Administration; D013375:Substance Withdrawal Syndrome / Q000503:physiopathology*; D013569:Synapses / Q000502:physiology*; D009435:Synaptic Transmission / Q000502:physiology*; D013844:Thiazoles / Q000494:pharmacology; D017557:Ventral Tegmental Area / Q000502:physiology
chemical_list: D001565:Benzoates; D002118:Calcium; D003513:Cycloheximide; D018691:Excitatory Amino Acid Antagonists; D005998:Glycine; D008734:Methoxyhydroxyphenylglycol; D011500:Protein Synthesis Inhibitors; D011725:Pyridines; D018091:Receptors, AMPA; D013844:Thiazoles; C472365:3-((2-methyl-1,3-thiazol-4-yl)ethynyl)pyridine; C079694:alpha-methyl-4-carboxyphenylglycine; C010117:3,4-dihydroxyphenylglycol
keywords: addiction; mGluR; medium spiny neurons; synaptic plasticity