title: Preparation of splicing competent nuclear extracts.
abstract: Splicing components play an essential role in mediating accurate and efficient splicing. The complexity of the spliceosome and its regulatory networks increase the difficulty of studying the splicing reaction in detail. Nuclear extracts derived from HeLa cells provide all of the obligatory components to carry out intron removal in vitro. This chapter describes the large-scale preparation of nuclear extract from HeLa cells.
authors.affiliation: Department of Microbiology & Molecular Genetics, University of California, Irvine, CA, USA.
authors.forename: Chiu-Ho T
authors.initials: CH
authors.lastname: Webb
authors.forename: Klemens J
authors.initials: KJ
authors.lastname: Hertel
mesh_terms: D002457:Cell Extracts / Q000235:genetics; D002467:Cell Nucleus / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D012326:RNA Splicing; D017381:Spliceosomes
chemical_list: D002457:Cell Extracts