title: Minimal effects of macromolecular crowding on an intrinsically disordered protein: a small-angle neutron scattering study.
abstract: Small-angle neutron scattering was used to study the effects of macromolecular crowding by two globular proteins, i.e., bovine pancreatic trypsin inhibitor and equine metmyoglobin, on the conformational ensemble of an intrinsically disordered protein, the N protein of bacteriophage λ. The λ N protein was uniformly labeled with (2)H, and the concentrations of D2O in the samples were adjusted to match the neutron scattering contrast of the unlabeled crowding proteins, thereby masking their contribution to the scattering profiles. Scattering from the deuterated λ N was recorded for samples containing up to 0.12 g/mL bovine pancreatic trypsin inhibitor or 0.2 g/mL metmyoglobin. The radius of gyration of the uncrowded protein was estimated to be 30 Å and was found to be remarkably insensitive to the presence of crowders, varying by <2 Å for the highest crowder concentrations. The scattering profiles were also used to estimate the fractal dimension of λ N, which was found to be ∼1.8 in the absence or presence of crowders, indicative of a well-solvated and expanded random coil under all of the conditions examined. These results are contrary to the predictions of theoretical treatments and previous experimental studies demonstrating compaction of unfolded proteins by crowding with polymers such as dextran and Ficoll. A computational simulation suggests that some previous treatments may have overestimated the effective volumes of disordered proteins and the variation of these volumes within an ensemble. The apparent insensitivity of λ N to crowding may also be due in part to weak attractive interactions with the crowding proteins, which may compensate for the effects of steric exclusion.
authors.affiliation: Department of Biology, University of Utah, Salt Lake City, Utah. Electronic address: goldenberg@biology.utah.edu.
authors.forename: David P
authors.initials: DP
authors.lastname: Goldenberg
authors.affiliation: Department of Biology, University of Utah, Salt Lake City, Utah.
authors.forename: Brian
authors.initials: B
authors.lastname: Argyle
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D007611:Aprotinin / Q000737:chemistry; D002417:Cattle; D006736:Horses; D064267:Intrinsically Disordered Proteins / Q000737:chemistry*; D008786:Metmyoglobin / Q000737:chemistry; D008969:Molecular Sequence Data; D033363:Neutron Diffraction; D053838:Scattering, Small Angle; D054334:Viral Regulatory and Accessory Proteins / Q000737:chemistry*
chemical_list: D064267:Intrinsically Disordered Proteins; C059417:N protein, Bacteriophage lambda; D054334:Viral Regulatory and Accessory Proteins; D008786:Metmyoglobin; D007611:Aprotinin