title: cAMP signaling affects irreversible attachment during biofilm formation by Pseudomonas aeruginosa PAO1.
abstract: Pseudomonas aeruginosa responds to environmental changes and regulates its life cycle from planktonic to biofilm modes of growth. The control of cell attachment to surfaces is one of the critical processes that determine this transition. Environmental signals are typically relayed to the cytoplasm by second messenger systems. We here demonstrated that the second messenger, cAMP, regulated the attachment of cells. Our results suggest cAMP inhibited the transition from reversible to irreversible attachment. Further analyses revealed that cell surface hydrophobicity, one of the key factors in cell attachment, was altered by cAMP.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba.
authors.forename: Kaori
authors.initials: K
authors.lastname: Ono
authors.forename: Rie
authors.initials: R
authors.lastname: Oka
authors.forename: Masanori
authors.initials: M
authors.lastname: Toyofuku
authors.forename: Ayane
authors.initials: A
authors.lastname: Sakaguchi
authors.forename: Masakaze
authors.initials: M
authors.lastname: Hamada
authors.forename: Shiomi
authors.initials: S
authors.lastname: Yoshida
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Nomura
mesh_terms: D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018441:Biofilms; D000242:Cyclic AMP / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D015398:Signal Transduction
chemical_list: D001426:Bacterial Proteins; D000242:Cyclic AMP