title: Evaluation of antioxidant and antiangiogenic properties of caesalpinia echinata extracts.
abstract: Natural products contain important combinations of ingredients, which may to some extent help to modulate the effects produced by oxidation substrates in biological systems. It is known that substances capable of modulating the action of these oxidants on tissue may be important allies in the control of neovascularization in pathological processes. The aim of this study was to evaluate the antioxidant and antiangiogenic properties of an ethanol extract of Caesalpinia echinata. The evaluation of antioxidant properties was tested using two methods (DPPH inhibition and sequestration of nitric oxide). The antiangiogenic properties were evaluated using the inflammatory angiogenesis model in the corneas of rats. The extract of C. echinata demonstrated a high capacity to inhibit free radicals, with IC50 equal to 42.404 µg/mL for the DPPH test and 234.2 µg/mL for nitric oxide. Moreover, it showed itself capable of inhibiting the inflammatory angiogenic response by 77.49%. These data suggest that biochemical components belonging to the extract of C. echinata interfere in mechanisms that control the angiogenic process, mediated by substrates belonging to the arachidonic acid cascade, although the data described above also suggest that the NO buffer may contribute to some extent to the reduction in the angiogenic response.
authors.affiliation: 1. Departamento de Farmácia, Universidade Federal de Pernambuco. Avenida Professor Moraes Rêgo, s/n Cidade Universitária, Recife, PE, 50670-420 Pernambuco, Brasil.
authors.forename: Elisangela Christhianne Barbosa
authors.initials: EC
authors.lastname: da Silva Gomes
authors.affiliation: 2. Departamento de morfologia e fisiologia animal, Universidade Federal Rural de Pernambuco. Rua Dom Manoel de Medeiros, S/N, Dois Irmãos, Recife, PE, 52171-900 Pernambuco, Brasil.
authors.forename: George Chaves
authors.initials: GC
authors.lastname: Jimenez
authors.affiliation: 3. Departamento de Bioquímica, Universidade Federal de Pernambuco. Avenida Professor Moraes Rêgo, s/n Cidade Universitária, Recife, PE, 50670-420 Pernambuco, Brasil.
authors.forename: Luis Claudio Nascimento
authors.initials: LC
authors.lastname: da Silva
authors.affiliation: 4. Departamento de morfologia e fisiologia animal, Universidade Federal Rural de Pernambuco. Rua Dom Manoel de Medeiros, S/N, Dois Irmãos, Recife, PE, 52171-900 Pernambuco, Brasil.
authors.forename: Fabrício Bezerra
authors.initials: FB
authors.lastname: de Sá
authors.affiliation: 5. Departamento de imunopatologia Keiso Asami (LIKA), Federal de Pernambuco. Avenida Professor Moraes Rêgo, s/n Cidade Universitária, Recife, PE, 50670-420 Pernambuco, Brasil.
authors.forename: Karen Pena Cavalcanti
authors.initials: KP
authors.lastname: de Souza
authors.affiliation: 6. Centro Brasileiro de Pesquisas Físicas, Rua Dr. Xavier Sigaud, 150, Urca, RJ, 22290-180 Rio de Janeiro, Brasil.
authors.forename: Gerson S
authors.initials: GS
authors.lastname: Paiva
authors.affiliation: 7. Departamento de Farmácia, Universidade Federal de Pernambuco. Avenida Professor Moraes Rêgo, s/n Cidade Universitária, Recife, PE, 50670-420 Pernambuco, Brasil.
authors.forename: Ivone Antônia
authors.initials: IA
authors.lastname: de Souza
keywords: Caesalpinia echinata; angiogenesis; antioxidant; natural products