title: Polymorphism of the inhibin βA gene and its relationship with superovulation traits in Chinese Holstein cows.
abstract: Inhibin is a major regulator of secretion of follicle-stimulating hormone, which is involved in follicular development and regulation of steroidogenesis in females. The objectives of this study were to detect polymorphisms of the bovine inhibin beta-A subunit (INHβA) gene and to evaluate its associations with superovulatory responses in 171 Chinese Holstein cows treated for superovulation. Polymerase chain reaction-restricted fragment length polymorphism revealed a C>T transition determining the StyI polymorphism at position 7639 in intron I of the bovine INHβA gene, and three genotypes (CC, CT, and TT) were detected. The frequencies of the three genotypes showed a tendency for CT > TT > CC, and this polymorphism was in Hardy-Weinberg equilibrium. Statistical analysis revealed no significant differences of least square means for superovulation traits among the three genotypes (P > 0.05). These results demonstrate, for the first time, that the detected loci of the INHβA gene have no significant effects on superovulation performance in Chinese Holstein cows.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi, China.
authors.forename: W C
authors.initials: WC
authors.lastname: Yang
authors.affiliation: Beijing Amber Embryo Technology Co. Ltd., Beijing, China.
authors.forename: S J
authors.initials: SJ
authors.lastname: Li
authors.affiliation: Beijing Amber Embryo Technology Co. Ltd., Beijing, China.
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, China yangliguo2006@yahoo.com.cn.
authors.forename: L G
authors.initials: LG
authors.lastname: Yang
mesh_terms: D000818:Animals; D016022:Case-Control Studies; D002417:Cattle / Q000235:genetics* / Q000502:physiology; D005260:Female; D028322:Inhibin-beta Subunits / Q000235:genetics*; D011110:Polymorphism, Genetic; D013480:Superovulation / Q000235:genetics*
chemical_list: D028322:Inhibin-beta Subunits