title: Lipidomic "deep profiling": an enhanced workflow to reveal new molecular species of signaling lipids.
abstract: Current mass spectrometry-based lipidomics aims to comprehensively cover wide ranges of lipid classes. We introduce a strategy to capture phospho-monoester lipids and improve the detection of long-chain base phosphates (LCB-Ps, e.g., sphingosine-1-phosphate). Ten novel LCB-Ps (d18:2, t20:1, odd carbon forms) were discovered and characterized in tissues from human and mouse, as well in D. melanogaster and S. cerevisiae. These findings have immediate relevance for our understanding of sphingosine-1-phosphate biosynthesis, signaling, and degradation.
authors.affiliation: Department of Biological Sciences, National University of Singapore , 117543, Singapore.
authors.forename: Pradeep
authors.initials: P
authors.lastname: Narayanaswamy
authors.forename: Sudhirkumar
authors.initials: S
authors.lastname: Shinde
authors.forename: Robert
authors.initials: R
authors.lastname: Sulc
authors.forename: Rachel
authors.initials: R
authors.lastname: Kraut
authors.forename: Gregory
authors.initials: G
authors.lastname: Staples
authors.forename: Chung Hwee
authors.initials: CH
authors.lastname: Thiam
authors.forename: Rudolf
authors.initials: R
authors.lastname: Grimm
authors.forename: Börje
authors.initials: B
authors.lastname: Sellergren
authors.forename: Federico
authors.initials: F
authors.lastname: Torta
authors.forename: Markus R
authors.initials: MR
authors.lastname: Wenk
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D013058:Mass Spectrometry; D051379:Mice; D012441:Saccharomyces cerevisiae; D015398:Signal Transduction
chemical_list: D008055:Lipids