title: Effect of equal daily doses achieved by different power densities of low-level laser therapy at 635 nm on open skin wound healing in normal and diabetic rats.
abstract: OBJECTIVE
  Despite the fact that the molecular mechanism of low-level laser therapy (LLLT) is not yet known, the exploitation of phototherapy in clinical medicine and surgery is of great interest. The present study investigates the effects of LLLT on open skin wound healing in normal and diabetic rats.
  
  
  METHODS
  Four round full-thickness skin wounds on dorsum were performed in male adult nondiabetic (n = 24) and diabetic (n = 24) Sprague-Dawley rats. AlGaInP (635 nm, wavelength; 5 J/cm(2), daily dose) was used to deliver power densities of 1, 5, and 15 mW/cm(2) three times daily until euthanasia.
  
  
  RESULTS
  PMNL infiltration was lower in the irradiated groups (15 mW/cm(2)). The synthesis and organisation of collagen fibres were consecutively enhanced in the 5 mW/cm(2) and 15 mW/cm(2) groups compared to the others in nondiabetic rats. In the diabetic group the only significant difference was recorded in the ratio PMNL/Ma at 15 mW/cm(2). A significant difference in the number of newly formed capillaries in the irradiated group (5, 15 mW/cm(2)) was recorded on day six after injury compared to the control group.
  
  
  CONCLUSIONS
  LLLT confers a protective effect against excessive inflammatory tissue response; it stimulates neovascularization and the early formation of collagen fibres.
authors.affiliation: 1st Department of Surgery, Pavol Jozef Šafárik University, Medical Faculty and Louis Pasteur University Hospital, Trieda SNP1, 040 11 Košice, Slovakia.
authors.forename: Róbert
authors.initials: R
authors.lastname: Kilík
authors.affiliation: 1st Department of Surgery, Pavol Jozef Šafárik University, Medical Faculty and Louis Pasteur University Hospital, Trieda SNP1, 040 11 Košice, Slovakia.
authors.forename: Lucia
authors.initials: L
authors.lastname: Lakyová
authors.affiliation: Department of Medical and Clinical Biophysics, Pavol Jozef Šafárik University, Medical Faculty, Košice, Slovakia.
authors.forename: Ján
authors.initials: J
authors.lastname: Sabo
authors.affiliation: Department of Cardiovascular Diseases, International Clinical Research Center, St. Anne's University Hospital and Masaryk University, Pekarska 53, 656 91 Brno, Czech Republic.
authors.forename: Peter
authors.identifier: 0000-0002-7069-3784
authors.initials: P
authors.lastname: Kruzliak
authors.affiliation: 1st Department of Internal Medicine, Pavol Jozef Šafárik University, Medical Faculty and Louis Pasteur University Hospital, Košice, Slovakia.
authors.forename: Kamila
authors.initials: K
authors.lastname: Lacjaková
authors.affiliation: Department of Surgery, Pavol Jozef Šafárik University, Medical Faculty and 1st Private Hospital, Košice-Šaca a.s., Košice, Slovakia.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Vasilenko
authors.affiliation: 1st Department of Surgery, Pavol Jozef Šafárik University, Medical Faculty and Louis Pasteur University Hospital, Trieda SNP1, 040 11 Košice, Slovakia.
authors.forename: Martina
authors.initials: M
authors.lastname: Vidová
authors.affiliation: Department of Forensic Medicine, Pavol Jozef Šafárik University, Medical Faculty, Košice, Slovakia.
authors.forename: František
authors.initials: F
authors.lastname: Longauer
authors.affiliation: 1st Department of Surgery, Pavol Jozef Šafárik University, Medical Faculty and Louis Pasteur University Hospital, Trieda SNP1, 040 11 Košice, Slovakia.
authors.forename: Jozef
authors.initials: J
authors.lastname: Radoňak
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000473:pathology / Q000628:therapy*; D006801:Humans; D007154:Immune System Diseases; D007249:Inflammation / Q000473:pathology / Q000628:therapy*; D007960:Leukocyte Disorders; D028022:Low-Level Light Therapy; D008297:Male; D010789:Phototherapy; D011829:Radiation Dosage; D051381:Rats; D012867:Skin / Q000293:injuries / Q000528:radiation effects; D014945:Wound Healing / Q000528:radiation effects