title: Regulation network and expression profiles of Epstein-Barr virus-encoded microRNAs and their potential target host genes in nasopharyngeal carcinomas.
abstract: Epstein-Barr virus (EBV) is associated with nasopharyngeal carcinoma (NPC) tumorigenesis. However, the mechanism(s) connecting EBV infection and NPC remain unclear. Recently, a new class of EBV microRNAs (miRNAs) has been described. To determine how EBV miRNAs control the expression of host genes, and to understand their potential role in NPC tumorigenesis, we profiled the expression of 44 mature EBV miRNAs and potential host genes in NPC and non-tumor nasopharyngeal epithelial tissues. We found that 40 EBV miRNAs from the BART transcript were highly expressed in NPC. Analysis of potential BART miRNA target genes revealed that 3140 genes and several important pathways might be involved in the carcinogenesis of NPC. A total of 105 genes with potential EBV miRNA binding sites were significantly downregulated, suggesting that EBV miRNAs may regulate these genes and contribute to NPC carcinogenesis. An EBV miRNA and host gene regulation network was generated to provide useful clues for validating of EBV miRNA functions in NPC tumorigenesis.
authors.affiliation: Hunan Provincial Tumor Hospital, Xiangya School of Medicine, Central South University, Changsha, 410013, China.|Key Laboratory of Carcinogenesis of Ministry of Health, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha, 410078, China.|Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center, The Third Xiangya Hospital, Central South University, Changsha, 410013, China.
authors.forename: ZhaoYang
authors.initials: Z
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Carcinogenesis of Ministry of Health, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha, 410078, China.|Key Laboratory of Information System Engineering, National University of Defense Technology, Changsha, 410073, China.
authors.forename: HongBin
authors.initials: H
authors.lastname: Huang
authors.affiliation: Key Laboratory of Carcinogenesis of Ministry of Health, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha, 410078, China.
authors.forename: LiLi
authors.initials: L
authors.lastname: Huang
authors.affiliation: Key Laboratory of Carcinogenesis of Ministry of Health, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha, 410078, China.
authors.forename: MengXi
authors.initials: M
authors.lastname: Sun
authors.affiliation: Key Laboratory of Carcinogenesis of Ministry of Health, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha, 410078, China.
authors.forename: QiJia
authors.initials: Q
authors.lastname: Yan
authors.affiliation: Key Laboratory of Carcinogenesis of Ministry of Health, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha, 410078, China.
authors.forename: YaLi
authors.initials: Y
authors.lastname: Song
authors.affiliation: Key Laboratory of Carcinogenesis of Ministry of Health, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha, 410078, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wei
authors.affiliation: Key Laboratory of Carcinogenesis of Ministry of Health, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha, 410078, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Bo
authors.affiliation: Key Laboratory of Carcinogenesis of Ministry of Health, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha, 410078, China.
authors.forename: ZhaoJian
authors.initials: Z
authors.lastname: Gong
authors.affiliation: Hunan Provincial Tumor Hospital, Xiangya School of Medicine, Central South University, Changsha, 410013, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Carcinogenesis of Ministry of Health, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha, 410078, China.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Li
authors.affiliation: Key Laboratory of Carcinogenesis of Ministry of Health, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha, 410078, China.
authors.forename: WenLing
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center, The Third Xiangya Hospital, Central South University, Changsha, 410013, China.
authors.forename: XiaYu
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Carcinogenesis of Ministry of Health, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha, 410078, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Xiang
authors.affiliation: Key Laboratory of Carcinogenesis of Ministry of Health, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha, 410078, China.
authors.forename: XiaoLing
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology and Center for Genetics and Molecular Medicine, School of Medicine, University of Louisville, Louisville, KY, 40202, USA.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Hunan Provincial Tumor Hospital, Xiangya School of Medicine, Central South University, Changsha, 410013, China. xiongwei@xysm.net.|Key Laboratory of Carcinogenesis of Ministry of Health, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha, 410078, China. xiongwei@xysm.net.|Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center, The Third Xiangya Hospital, Central South University, Changsha, 410013, China. xiongwei@xysm.net.
authors.forename: Wei
authors.initials: W
authors.lastname: Xiong
authors.affiliation: Hunan Provincial Tumor Hospital, Xiangya School of Medicine, Central South University, Changsha, 410013, China. ligy@xysm.net.|Key Laboratory of Carcinogenesis of Ministry of Health, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha, 410078, China. ligy@xysm.net.|Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center, The Third Xiangya Hospital, Central South University, Changsha, 410013, China. ligy@xysm.net.
authors.forename: GuiYuan
authors.initials: G
authors.lastname: Li
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D015967:Gene Expression Regulation, Viral; D053263:Gene Regulatory Networks; D057809:HEK293 Cells; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000502:physiology; D054884:Host-Pathogen Interactions / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D009305:Nasopharynx / Q000378:metabolism / Q000473:pathology; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D059467:Transcriptome; D044844:beta-Transducin Repeat-Containing Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C471644:BTRC protein, human; D035683:MicroRNAs; D012367:RNA, Viral; D044844:beta-Transducin Repeat-Containing Proteins