title: Evaluation of in vitro antimalarial activity of different extracts of Artemisia aucheri Boiss. and A. armeniaca Lam. and fractions of the most potent extracts.
abstract: Ten extracts with different polarity from two Iranian Artemisia species, A. armeniaca Lam. and A. aucheri Boiss, were screened for their antimalarial properties by in vitro   β -hematin formation assay. Dichloromethane (DCM) extracts of both plants showed significant antimalarial activities with IC50 values of 1.36±0.01 and 1.83±0.03 mg/mL and IC90 values of 2.12±0.04 and 2.62±0.09 mg/mL for A. armeniaca and A. aucheri, respectively. Bioactivity-guided fractionation of DCM extracts of both plants by vacuum liquid chromatography (VLC) over silica gel with solvent mixtures of increasing polarities afforded seven fractions. Two fractions from DCM extract of A. armeniaca and four fractions from DCM extract of A. aucheri showed potent antimalarial activity with reducing IC50 and IC90 values compared to extracts. The most potent fraction belonged to DCM extract of A. armeniaca with IC50 and IC90 values of 0.47±0.006 and 0.71±0.006 mg/mL, respectively.
authors.affiliation: Novel Drug Delivery Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah 67346-67149, Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Mojarrab
authors.affiliation: Novel Drug Delivery Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah 67346-67149, Iran ; Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah 67346-67149, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Shiravand
authors.affiliation: Drug Applied Research Centre, Tabriz University of Medical Sciences, Tabriz 51656-65811, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Delazar
authors.affiliation: Medical Philosophy and History Research Center, Tabriz University of Medical Sciences, Tabriz 371-51665, Iran.
authors.forename: Fariba
authors.identifier: 0000-0002-1382-6106
authors.initials: F
authors.lastname: Heshmati Afshar
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000302:isolation & purification* / Q000378:metabolism*; D019306:Artemisia; D004353:Drug Evaluation, Preclinical / Q000379:methods; D006420:Hemeproteins / Q000032:analysis / Q000378:metabolism*; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000302:isolation & purification* / Q000378:metabolism*; D013552:Swine
chemical_list: D000962:Antimalarials; D006420:Hemeproteins; D010936:Plant Extracts; C020753:hemozoin