title: A novel reporter system for neutralizing and enhancing antibody assay against dengue virus.
abstract: BACKGROUND
  Dengue virus (DENV) still poses a global public health threat, and no vaccine or antiviral therapy is currently available. Antibody plays distinct roles in controlling DENV infections. Neutralizing antibody is protective against DENV infection, whereas sub-neutralizing concentration of antibody can increase DENV infection, termed antibody-dependent enhancement (ADE). Plaque-based assay represents the most widely accepted method measuring neutralizing or enhancing antibodies.
  
  
  RESULTS
  In this study, a novel reporter virus-based system was developed for measuring neutralization and ADE activity. A stable Renilla luciferase reporter DENV (Luc-DENV) that can produce robust luciferase signals in BHK-21 and K562 cells were used to establish the assay and validated against traditional plaque-based assay. Luciferase value analysis using various known DENV-specific monoclonal antibodies showed good repeatability and a well linear correlation with conventional plaque-based assays. The newly developed assay was finally validated with clinical samples from infected animals and individuals.
  
  
  CONCLUSIONS
  This reporter virus-based assay for neutralizing and enhancing antibody evaluation is rapid, lower cost, and high throughput, and will be helpful for laboratory detection and epidemiological investigation for DENV antibodies.
authors.forename: Ke-Yu
authors.initials: KY
authors.lastname: Song
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.forename: Zhen-You
authors.initials: ZY
authors.lastname: Jiang
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Li
authors.forename: Yong-Qiang
authors.initials: YQ
authors.lastname: Deng
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.forename: Shun-Ya
authors.initials: SY
authors.lastname: Zhu
authors.forename: Pei-Yong
authors.initials: PY
authors.lastname: Shi
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Fu-Chun
authors.initials: FC
authors.lastname: Zhang
authors.forename: E-De
authors.initials: ED
authors.lastname: Qin
authors.affiliation: Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China. qincf@bmi.ac.cn.
authors.forename: Cheng-Feng
authors.initials: CF
authors.lastname: Qin
mesh_terms: D000818:Animals; D019138:Antibodies, Blocking / Q000276:immunology*; D057134:Antibodies, Neutralizing / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D002460:Cell Line; D003716:Dengue Virus / Q000276:immunology*; D017930:Genes, Reporter; D006801:Humans; D007118:Immunoassay / Q000379:methods; D049410:Luciferases, Renilla / Q000032:analysis; D015203:Reproducibility of Results; D014773:Virology / Q000379:methods*
chemical_list: D019138:Antibodies, Blocking; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D049410:Luciferases, Renilla