title: Method Validation for Analysis of Simvastatin in Human Plasma Using Liquid Chromatography Tandem Mass Spectrometry (LC-MS-MS).
abstract: BACKGROUND
  The Liquid Chromatography Tandem Mass Spectrometry (LC-MS-MS) for determination simvastatin in human plasma has been developed after extraction by by ethyl acetate and hexane (90/10%, v/v) using lovastatin as internal standard.
  
  
  METHODS
  The mobile phase consisting of mixture of acetonitrile and water (75/25%, v/v) 500μL/min by separated the solutes on a C18 column.
  
  
  CONCLUSIONS
  The lower limit of quantitation of 0.25 ng/mL was achieved when the calibration curve was linear from 0.25-50 ng/mL. The entire run time for analysis was only 6 min. The quantitation in the selective reaction monitoring (SRM) in positive ion mode, the daughter ions m/z 325 for simvastatin and m/z 285 for lovastatin were used. The Parent ions in positive ion mode were m/z 441.3 for simvastatin and m/z 405.1 for lovastatin. The intra-day coefficients of variation were less than 14% while the inter-day coefficients of variation were less than 10%.
  
  
  CONCLUSIONS
  The LC-MS-MS detection is sensitive due to its capability to eliminate interferences from endogenous components.
authors.affiliation: (a) School of Pharmacy, Universiti Sains Malaysia. (b) College of Pharmacy, King Khalid University, Abha, Kingdom of Saudi Arabia. (c) Department of Pharmacy, Medical school in Thamar University , Republic of Yemen .
authors.forename: Khaled M
authors.initials: KM
authors.lastname: Alakhali
keywords: Human plasma; Liquid Chromatography Tandem Mass Spectrometry; Simvastatin