title: Preventing disulfide bond formation weakens non-covalent forces among lysozyme aggregates.
abstract: Nonnative disulfide bonds have been observed among protein aggregates in several diseases like amyotrophic lateral sclerosis, cataract and so on. The molecular mechanism by which formation of such bonds promotes protein aggregation is poorly understood. Here in this work we employ previously well characterized aggregation of hen eggwhite lysozyme (HEWL) at alkaline pH to dissect the molecular role of nonnative disulfide bonds on growth of HEWL aggregates. We employed time-resolved fluorescence anisotropy, atomic force microscopy and single-molecule force spectroscopy to quantify the size, morphology and non-covalent interaction forces among the aggregates, respectively. These measurements were performed under conditions when disulfide bond formation was allowed (control) and alternatively when it was prevented by alkylation of free thiols using iodoacetamide. Blocking disulfide bond formation affected growth but not growth kinetics of aggregates which were ∼50% reduced in volume, flatter in vertical dimension and non-fibrillar in comparison to control. Interestingly, single-molecule force spectroscopy data revealed that preventing disulfide bond formation weakened the non-covalent interaction forces among monomers in the aggregate by at least ten fold, thereby stalling their growth and yielding smaller aggregates in comparison to control. We conclude that while constrained protein chain dynamics in correctly disulfide bonded amyloidogenic proteins may protect them from venturing into partial folded conformations that can trigger entry into aggregation pathways, aberrant disulfide bonds in non-amyloidogenic proteins (like HEWL) on the other hand, may strengthen non-covalent intermolecular forces among monomers and promote their aggregation.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam, India.
authors.forename: Vijay Kumar
authors.initials: VK
authors.lastname: Ravi
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam, India.
authors.forename: Mohit
authors.initials: M
authors.lastname: Goel
authors.affiliation: Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai, Maharashtra, India.
authors.forename: Hema Chandra
authors.initials: HC
authors.lastname: Kotamarthi
authors.affiliation: Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai, Maharashtra, India.
authors.forename: Sri Rama Koti
authors.initials: SR
authors.lastname: Ainavarapu
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam, India.
authors.forename: Rajaram
authors.initials: R
authors.lastname: Swaminathan
mesh_terms: D000682:Amyloid / Q000378:metabolism; D000818:Animals; D016880:Anisotropy; D052160:Benzothiazoles; D003460:Crystallization; D004220:Disulfides / Q000378:metabolism*; D057927:Hydrophobic and Hydrophilic Interactions; D018625:Microscopy, Atomic Force; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism*; D055585:Physical Phenomena; D020836:Protein Structure, Quaternary; D013050:Spectrometry, Fluorescence; D013844:Thiazoles / Q000378:metabolism
chemical_list: D000682:Amyloid; D052160:Benzothiazoles; D004220:Disulfides; D013844:Thiazoles; C009462:thioflavin T; C421864:hen egg lysozyme; D009113:Muramidase