title: Effects of lipid and propionic acid infusions on feed intake of lactating dairy cows.
abstract: Propionic acid is more hypophagic for cows with elevated hepatic acetyl coenzyme A (CoA) concentration in the postpartum period. The objective of this experiment was to evaluate the interaction of hepatic acetyl CoA concentration, which is elevated by intravenous lipid infusion, and intraruminal propionic acid infusion on feed intake and feeding behavior responses of lactating cows. Eight multiparous, ruminally cannulated, Holstein dairy cows past peak lactation were used in a replicated 4×4 Latin square experiment with a 2×2 factorial arrangement of treatments. Treatments were propionic acid (PI) infused intraruminally at 0.5mol/h for 18h starting 6h before feeding and behavior monitoring or sham control (CO), and intravenous jugular infusion of lipid (LI, Intralipid 20%; Baxter US, Deerfield, IL) or saline (SI, 0.9% NaCl; Baxter US) infused at 250mL/h for 12h before feeding and behavior monitoring, and then 500mL/h for 12h after feeding. Changes in plasma concentrations of metabolites and hormones and hepatic acetyl CoA from before infusion until the end of infusion were evaluated. We observed a tendency for an interaction between PI and LI for the change in plasma nonesterified fatty acid (NEFA) concentration from the preliminary day to the end of the infusion period. Infusion of propionic acid decreased dry matter intake (DMI) 15% compared with CO, but lipid infusion did not affect DMI over the 12h following feeding. Infusion of propionic acid tended to decrease hepatic acetyl CoA concentration from the preliminary day to the end of the infusion compared with CO, consistent with PI decreasing DMI by stimulating oxidation of acetyl CoA. Contrary to our expectations, LI did not increase concentration of NEFA or β-hydroxybutyrate in plasma, concentration of acetyl CoA in the liver, or milk fat yield, suggesting that the infused lipid was stored or oxidized by extra-hepatic tissues. As a result, we detected no interaction between PI and LI for DMI. Although the effect of PI on DMI was consistent with our previous results, this lipid infusion model using cows past peak lactation was not useful to simulate the lipolytic state of cows in the postpartum period in this experiment.
authors.affiliation: Department of Animal Science, Michigan State University, East Lansing 48824-1225.
authors.forename: S E
authors.initials: SE
authors.lastname: Stocks
authors.affiliation: Department of Animal Science, Michigan State University, East Lansing 48824-1225. Electronic address: allenm@msu.edu.
authors.forename: M S
authors.initials: MS
authors.lastname: Allen
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000097:blood; D000105:Acetyl Coenzyme A / Q000378:metabolism; D000821:Animal Feed; D000818:Animals; D002417:Cattle; D004032:Diet / Q000662:veterinary; D004041:Dietary Fats / Q000008:administration & dosage*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005247:Feeding Behavior; D005260:Female; D006728:Hormones / Q000097:blood; D006863:Hydrogen-Ion Concentration; D007774:Lactation; D008066:Lipolysis; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008892:Milk / Q000737:chemistry; D049590:Postpartum Period; D011422:Propionates / Q000008:administration & dosage*; D012417:Rumen / Q000187:drug effects / Q000378:metabolism
chemical_list: D004041:Dietary Fats; D005230:Fatty Acids, Nonesterified; D006728:Hormones; D011422:Propionates; D000105:Acetyl Coenzyme A; C029658:propionic acid; D020155:3-Hydroxybutyric Acid
keywords: hepatic oxidation; hypophagia; lipid metabolism; propionate metabolism