title: Assessment of cellular viability on calcium sulphate/hydroxyapatite injectable scaffolds.
abstract: Cements for maxillofacial reconstruction of jaw defects through calcification of rotated muscle have been tested. The objective of this study was to investigate the visibility of loading of two types of commercially available cements, Cerament(™) Spine Support and Cerament Bone Void Filler with mesenchymal cells and cytokines (bone morphogenetic protein) to act as a biomimetic scaffolding for future clinical application. Determination of basic biocompatibility (cell viability) using methyl thiazolyl tetrazolium and live/dead assay was carried out using MG-63 cells at various time points. Next, in order to inform potential subsequent in vivo experiments, a collagen tissue mimic was used for characterization of rabbit mesenchymal stromal cells using immunofluorescent cytoskeleton staining, and simultaneous and then sequential injection of Cerament Spine Support cement and cells into collagen gels. Results indicated that Cerament Spine Support was more biocompatible and that sequential injection of cement and then rabbit mesenchymal stromal cells into the tissue mimics is an optimal approach for clinical applications.
authors.affiliation: Glasgow Dental Hospital & School, University of Glasgow, Glasgow, UK.
authors.forename: Randa
authors.initials: R
authors.lastname: Alfotawi
authors.affiliation: Glasgow Dental Hospital & School, University of Glasgow, Glasgow, UK.
authors.forename: Kurt
authors.initials: K
authors.lastname: Naudi
authors.affiliation: Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Dalby
authors.affiliation: School of Engineering, University of Glasgow, Glasgow, UK.
authors.forename: K Elizabeth
authors.initials: KE
authors.lastname: Tanner
authors.affiliation: Regional Maxillofacial Unit, Southern General Hospital, Glasgow, UK.
authors.forename: Jeremy D
authors.initials: JD
authors.lastname: McMahon
authors.affiliation: Glasgow Dental Hospital & School, University of Glasgow, Glasgow, UK.
authors.forename: Ashraf
authors.initials: A
authors.lastname: Ayoub
keywords: Calcium sulphate; cell culture; hydroxyapatite; injectable; mesenchymal stromal cell