title: A β-secretase (BACE1)-inhibiting C-methylrotenoid induced by yeast elicitation in Abronia nana suspension cultures.
abstract: Suspension cultures of Abronia nana were established to produce C-methylisoflavones. Treatment of the A. nana cultures with yeast elicitor induced boeravinone E (1), with maximum induction at 24 h after elicitor treatment. Of the biotic and abiotic elicitors tested, yeast extract gave the strongest induction of 1. The IC50 value of 1 against β-secretase (β-amyloid cleaving enzyme-1) was 5.57 μM. Other proteases such as trypsin, chymotrypsin, and elastase were not inhibited by concentrations up to 1.0 mM, indicating that inhibition of β-secretase was specific. 1 was noncompetitive in Dixon plot, and Ki value was 3.79 μM.
authors.affiliation: Research Institute of Pharmaceutical Sciences, College of Pharmacy, Kyungpook National University, Daehak-ro 80, Sankyuk-dong, Daegu, 702-701, Republic of Korea.
authors.forename: Sang-In
authors.initials: SI
authors.lastname: Kim
authors.forename: Eun-Ju
authors.initials: EJ
authors.lastname: Yang
authors.forename: Se-Hoon
authors.initials: SH
authors.lastname: Park
authors.forename: Chang-Kil
authors.initials: CK
authors.lastname: Kim
authors.forename: Kyung-Sik
authors.initials: KS
authors.lastname: Song
mesh_terms: D053829:Amyloid Precursor Protein Secretases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002478:Cells, Cultured; D003470:Culture Media / Q000378:metabolism*; D005419:Flavonoids / Q000096:biosynthesis*; D039281:Nyctaginaceae / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D010940:Plant Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015003:Yeasts / Q000737:chemistry*
chemical_list: D003470:Culture Media; D005419:Flavonoids; D010940:Plant Proteins; C560378:boeravinone E; D053829:Amyloid Precursor Protein Secretases