title: Blood group substances as potential therapeutic agents for the prevention and treatment of infection with noroviruses proving novel binding patterns in human tissues.
abstract: Blood group-related glycans determining ABO and Lewis blood groups are known to function as attachment factors for most of the norovirus (NoV) strains. To identify binding specificity of each NoV, recombinant norovirus-like particles (VLPs) and human saliva samples with different ABO, Lewis phenotypes and secretor status have been commonly applied. When binding specificities of VLPs prepared from 16 different genotypes of NoVs in GI and GII genogroups were characterized in samples of human gastric mucosa compared to human saliva based on blood group phenotypes, considerable differences were observed for several strains. Novel binding specificities determined by an ELISA using preparations from human gastric mucosa were also ascertained by immunohistochemical analyses using human jejunal mucosa, widely believed to be susceptible to NoV infection. Further, A, B and O(H) blood group substances prepared from porcine and squid tissues were found to be effective for preventing ABO blood group-specific binding of VLPs to both saliva and mucosa samples. Therefore, these blood group substances might have potential for the prevention and treatment of NoV infection.
authors.affiliation: Department of General Surgical Science, Gunma University Graduate School of Medicine, Maebashi, Japan ; Tokushima Research Institute, Otsuka Pharmaceutical Co. Ltd., Tokushima, Japan.
authors.forename: Shin
authors.initials: S
authors.lastname: Yazawa
authors.affiliation: Department of General Surgical Science, Gunma University Graduate School of Medicine, Maebashi, Japan.
authors.forename: Takehiko
authors.initials: T
authors.lastname: Yokobori
authors.affiliation: Laboratory of Glycobiology, Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Japan.
authors.forename: Gen
authors.initials: G
authors.lastname: Ueta
authors.affiliation: Department of Diagnostic Pathology, Gunma University Graduate School of Medicine, Maebashi, Japan.
authors.forename: Munenori
authors.initials: M
authors.lastname: Ide
authors.affiliation: Department of General Surgical Science, Gunma University Graduate School of Medicine, Maebashi, Japan.
authors.forename: Bolag
authors.initials: B
authors.lastname: Altan
authors.affiliation: Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan ; Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan.
authors.forename: Aksara
authors.initials: A
authors.lastname: Thongprachum
authors.affiliation: Tokushima Research Institute, Otsuka Pharmaceutical Co. Ltd., Tokushima, Japan.
authors.forename: Toyo
authors.initials: T
authors.lastname: Nishimura
authors.affiliation: Department of Legal Medicine, Gunma University Graduate School of Medicine, Maebashi, Japan.
authors.forename: Tamiko
authors.initials: T
authors.lastname: Nakajima
authors.affiliation: Department of Legal Medicine, Gunma University Graduate School of Medicine, Maebashi, Japan.
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Kominato
authors.affiliation: Department of General Surgical Science, Gunma University Graduate School of Medicine, Maebashi, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Asao
authors.affiliation: JIMRO Co., Ltd., Tokyo, Japan.
authors.forename: Abby R
authors.initials: AR
authors.lastname: Saniabadi
authors.affiliation: Laboratory of Glycobiology, Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Furukawa
authors.affiliation: Department of General Surgical Science, Gunma University Graduate School of Medicine, Maebashi, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kuwano
authors.affiliation: Inserm, UMR892; CNRS, UMR 6299; University of Nantes, Nantes, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Le Pendu
authors.affiliation: Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan ; Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ushijima
mesh_terms: D000818:Animals; D001789:Blood Group Antigens / Q000378:metabolism* / Q000627:therapeutic use; D017250:Caliciviridae Infections / Q000097:blood* / Q000517:prevention & control*; D049832:Decapodiformes; D004797:Enzyme-Linked Immunosorbent Assay; D005753:Gastric Mucosa / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D029322:Norovirus / Q000378:metabolism*; D011134:Polysaccharides / Q000097:blood / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D012463:Saliva / Q000378:metabolism; D013552:Swine; D014771:Virion / Q000378:metabolism
chemical_list: D001789:Blood Group Antigens; D011134:Polysaccharides