title: Lack of association between mannose binding lectin and antibody responses after acellular pertussis vaccinations.
abstract: BACKGROUND
  Mannose-binding lectin (MBL) is one of the key molecules in innate immunity and its role in human vaccine responses is poorly known. This study aimed to investigate the possible association of MBL polymorphisms with antibody production after primary and booster vaccinations with acellular pertussis vaccines in infants and adolescents.
  
  
  RESULTS
  Five hundred and sixty eight subjects were included in this study. In the adolescent cohort 355 subjects received a dose of diphtheria and tetanus toxoids and acellular pertussis (dTpa) vaccine ten years previously. Follow-up was performed at 3, 5 and 10 years. Infant cohort consisted of 213 subjects, who had received three primary doses of DTaP vaccine at 3, 5, and 12 months of age according to Finnish immunization program. Blood samples were collected before the vaccinations at 2,5 months of age and after the vaccinations at 13 months and 2 years of age. Concentrations of IgG antibodies to pertussis toxin, filamentous hemagglutinin, and pertactin and antibodies to diphtheria and tetanus toxoids were measured by standardized enzyme-linked immunosorbant assay. Single nucleotide polymorphisms of MBL2 gene exon1 (codons 52, 54, 57) were examined. MBL serum concentration was also measured from the adolescent cohort. No association was found with MBL2 exon 1 polymorphisms and antibody responses against vaccine antigens, after primary and booster dTpa vaccination.
  
  
  CONCLUSIONS
  This study indicates that MBL polymorphisms do not affect the production and persistence of antibodies after acellular pertussis vaccination. Our finding also suggests that MBL might not be involved in modulating antibody responses to the vaccines made of purified bacterial proteins.
authors.affiliation: Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare (THL), Turku, Finland.
authors.forename: Kirsi
authors.initials: K
authors.lastname: Gröndahl-Yli-Hannuksela
authors.affiliation: Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare (THL), Turku, Finland.
authors.forename: Juho
authors.initials: J
authors.lastname: Vuononvirta
authors.affiliation: Department of Pediatrics, Turku University Hospital, Turku, Finland.
authors.forename: Ville
authors.initials: V
authors.lastname: Peltola
authors.affiliation: Department of Pediatrics, Turku University Hospital, Turku, Finland.
authors.forename: Jussi
authors.initials: J
authors.lastname: Mertsola
authors.affiliation: Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare (THL), Turku, Finland.
authors.forename: Qiushui
authors.initials: Q
authors.lastname: He
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D022681:Diphtheria-Tetanus-acellular Pertussis Vaccines / Q000276:immunology*; D005260:Female; D005838:Genotype; D006801:Humans; D007117:Immunization, Secondary; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology*; D007223:Infant; D008297:Male; D037601:Mannose-Binding Lectin / Q000097:blood* / Q000235:genetics*; D014611:Vaccination
chemical_list: D022681:Diphtheria-Tetanus-acellular Pertussis Vaccines; D007074:Immunoglobulin G; D037601:Mannose-Binding Lectin