title: Determination of ractopamine in pig hair using liquid chromatography with tandem mass spectrometric detection.
abstract: A quantitative analytical procedure for the determination of ractopamine in pig hair has been developed and validated. The hair samples were washed and incubated at 75°C with isoxuprine and hair extraction buffer. The drug present was quantified using mixed solid-phase extraction and liquid chromatography with tandem mass spectrometric detection. The limit of quantization (LOQ) was 10pg/mg and the intra-day precision at 25pg/mg and 750pg/mg was 0.49% and 2.8% respectively. Inter-day precision was 0.88% and 3.52% at the same concentrations. The hair extraction percentage recovery at 25pg/mg and 50ng/mL was 99.47% and 103.83% respectively. The extraction percentage recovery at 25pg/mg and 50ng/mg was 93.52% and 100.26% respectively. Our results showed that ractopamine residues persist in hair in 24days of withdrawal and also showed the possibility to test ractopamine from pig hair samples.
authors.affiliation: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou 510070, China.
authors.forename: Junlin
authors.initials: J
authors.lastname: Wu
authors.affiliation: Guangzhou Wondfo Biotech. Co., Ltd., Guangzhou Accurate and Correct Test Co., Ltd., Guangzhou, Guangdong 510643, China; School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Liu
authors.affiliation: Guangzhou Wondfo Biotech. Co., Ltd., Guangzhou Accurate and Correct Test Co., Ltd., Guangzhou, Guangdong 510643, China. Electronic address: microwjl@yahoo.com.
authors.forename: Yunping
authors.initials: Y
authors.lastname: Peng
mesh_terms: D000318:Adrenergic beta-Agonists / Q000493:pharmacokinetics; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D006197:Hair / Q000737:chemistry*; D057230:Limit of Detection; D008297:Male; D010627:Phenethylamines / Q000493:pharmacokinetics*; D052616:Solid Phase Extraction; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors
chemical_list: D000318:Adrenergic beta-Agonists; D010627:Phenethylamines; C060897:ractopamine
keywords: Hair; Liquid chromatography; Ractopamine; Validation