title: Designing and testing the activities of TAL effector nucleases.
abstract: Transcription activator-like effector nucleases (TALENs) have rapidly developed into a powerful tool for genome editing. To avoid labor-intensive and time-consuming experimental screening for active TALENs, a scoring system can help select optimal target sites. Here we describe a procedure to design active TALENs using a scoring system named Scoring Algorithm for Predicted TALEN Activity (SAPTA) and a method to test the activity of individual and pairs of TALENs.
authors.affiliation: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
authors.forename: Yanni
authors.initials: Y
authors.lastname: Lin
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Cradick
authors.forename: Gang
authors.initials: G
authors.lastname: Bao
mesh_terms: D053837:DNA Cleavage; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000235:genetics / Q000378:metabolism; D004720:Endonucleases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018390:Gene Targeting; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D014162:Transfection
chemical_list: D004268:DNA-Binding Proteins; D004720:Endonucleases; D003850:Deoxyribonuclease I