title: Molecular cloning and expression analysis of prostaglandin E receptor 2 gene in cashmere goat (Capra hircus) skin during hair follicle development.
abstract: As a member of the four subtypes of receptors for prostaglandin E2 (PGE2), prostaglandin E receptor 2 (PTGER2) is in the family of G-protein coupled receptors and has been characterized to be involved in the development and growth of hair follicles. In this study, we cloned and characterized the full-length coding sequence (CDS) of PTGER2 gene from cashmere goat skin. The entire open reading frame (ORF) of PTGER2 gene was 1047 bp and encoded 348 amino acid residues. The deduced protein contained one G-protein coupled receptors family 1 signature, seven transmembrane domains, and other potential sites. Tissue expression analysis showed that PTGER2 gene was expressed strongly in the skin. The general expression tendency of PTGER2 gene at different hair follicle developmental stages in the skin was gradually decreased from anagen to catagen to telogen. After comparing with the expression of BMP4 gene and related reports, we further presume that it seems to have a relationship between the hair follicle cycle and the expression level of PTGER2 gene in cashmere goat skin.
authors.affiliation: a College of Life Science and Technology, Yancheng Teachers University , Yancheng , Jiangsu Province , P. R. China.
authors.forename: Rong-Qing
authors.initials: RQ
authors.lastname: Geng
authors.forename: Chao
authors.initials: C
authors.lastname: Yuan
authors.forename: Yu-Lin
authors.initials: YL
authors.lastname: Chen
mesh_terms: D000375:Aging / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006041:Goats; D018859:Hair Follicle / Q000737:chemistry / Q000502:physiology*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D058307:Receptors, Prostaglandin E, EP2 Subtype / Q000737:chemistry / Q000502:physiology*; D014018:Tissue Distribution
chemical_list: D058307:Receptors, Prostaglandin E, EP2 Subtype