title: Micropatterning on micropost arrays.
abstract: Micropatterning of cells can be used in combination with microposts to control cell shape or cell-to-cell interaction while measuring cellular forces. The protocols in this chapter describe how to make SU8 masters for stamps and microposts, how to use soft lithography to replicate these structures in polydimethylsiloxane, and how to functionalize the surface of the microposts for cell attachment.
authors.affiliation: Department of Mechanical Engineering, University of Washington, Seattle, Washington, USA; Department of Bioengineering, University of Washington, Seattle, Washington, USA.
authors.forename: Nathan J
authors.initials: NJ
authors.lastname: Sniadecki
authors.affiliation: Department of Mechanical Engineering, University of Washington, Seattle, Washington, USA.
authors.forename: Sangyoon J
authors.initials: SJ
authors.lastname: Han
authors.affiliation: Department of Mechanical Engineering, University of Washington, Seattle, Washington, USA.
authors.forename: Lucas H
authors.initials: LH
authors.lastname: Ting
authors.affiliation: Department of Mechanical Engineering, University of Washington, Seattle, Washington, USA.
authors.forename: Shirin
authors.initials: S
authors.lastname: Feghhi
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002450:Cell Communication; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D004852:Epoxy Compounds / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D055616:Microtechnology / Q000379:methods*; D011108:Polymers / Q000737:chemistry; D013314:Stress, Mechanical; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D004852:Epoxy Compounds; D011108:Polymers; C520895:SU-8 compound
keywords: Cell–cell junctions; Mechanobiology; Mechanotransduction; Microcontact printing; Micropillars; Microposts; Nanoposts; Traction forces; Tugging forces