title: Comparision of uroprotective activity of reduced glutathione with mesna in ifosfamide induced hemorrhagic cystitis in rats.
abstract: BACKGROUND
  Ifosfamide (IFO) is widely used DNA-alkylating agents in cancer chemotherapy for management of solid tumors and hematological malignancies. However, hemorrhagic cystitis limits the use of IFO.
  
  
  OBJECTIVE
  To compare the efficiency of reduced glutathione with 2-Mesna in reducing Ifosfamide (IFO) induced hemorrhagic cystitis (HC) in wistar rats.
  
  
  METHODS
  Ifosfamide and 2-Mesna were dissolved in sterile water for injection and administered to wistar rats of albino strains. The rats were randomly assigned to one of the four groups of 6 rats each: Group I: Vehicle control; Group II: 120 mg/kg of IFO alone by intraperitoneal injection (i.p); Group III: 40 mg/kg Mesna i.p., at the same time and at 4 and 8 h after IFO administration; Group IV: 500 mg/kg of glutathione i.p., 30 min prior to IFO as above. The animals were observed for 5 days. On 6th day, rats were sacrificed by dissecting the intrajugular vein. The bladders were macroscopically and histopathologically evaluated.
  
  
  RESULTS
  Control animals had normal bladders with assigned scores of '0' for the three parameters of edema, hemorrhage and histopathological changes. All the animals receiving IFO (group II) had evidence of HC as evidenced by alterations of edema and hemorrhages. These alterations were almost abolished (P < 0.001) by the glutathione (group III) or Mesna (group IV) in IFO-treated animals.
  
  
  CONCLUSIONS
  Glutathione could be as useful as Mesna in the preventive management of IFO-induced HC.
authors.affiliation: Deccan School of Pharmacy, Hyderabad, Andhra Pradesh, India.
authors.forename: Syed Amir
authors.initials: SA
authors.lastname: Ali
authors.affiliation: Bharat Institute of Technology, Hyderabad, Andhra Pradesh, India.
authors.forename: Sandeep Kumar
authors.initials: SK
authors.lastname: Danda
authors.affiliation: Deccan School of Pharmacy, Hyderabad, Andhra Pradesh, India.
authors.forename: Syed Abdul Azeez
authors.initials: SA
authors.lastname: Basha
authors.affiliation: Deccan School of Pharmacy, Hyderabad, Andhra Pradesh, India.
authors.forename: Asif
authors.initials: A
authors.lastname: Rasheed
authors.affiliation: Deccan School of Pharmacy, Hyderabad, Andhra Pradesh, India.
authors.forename: Osman
authors.initials: O
authors.lastname: Ahmed
authors.affiliation: Deccan School of Pharmacy, Hyderabad, Andhra Pradesh, India.
authors.forename: M Muqtedar
authors.initials: MM
authors.lastname: Ahmed
mesh_terms: D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000009:adverse effects; D003556:Cystitis / Q000139:chemically induced / Q000517:prevention & control*; D005978:Glutathione / Q000494:pharmacology*; D006470:Hemorrhage / Q000139:chemically induced / Q000517:prevention & control*; D007069:Ifosfamide / Q000009:adverse effects*; D015080:Mesna / Q000009:adverse effects*; D020011:Protective Agents / Q000009:adverse effects; D051381:Rats; D017208:Rats, Wistar; D001743:Urinary Bladder / Q000187:drug effects* / Q000473:pathology
chemical_list: D018906:Antineoplastic Agents, Alkylating; D020011:Protective Agents; D005978:Glutathione; D015080:Mesna; D007069:Ifosfamide
keywords: Glutathione; hemorrhagic cystitis; ifosfamide; solid tumors