title: Arsenic accumulating bacteria isolated from soil for possible application in bioremediation.
abstract: Twenty six arsenic (As) resistant bacterial strains were isolated from As contaminated paddy soil of West Bengal, India. Among them, 10 isolates exhibited higher As resistance capacity and could grow in concentration of 12000 mg I-1 of arsenate (AsV) and 2000 mg l-1 of arsenite (Aslll) in growing medium. Maximum growth was observed at 1000 mg I-1and 100 mg I-1 in case of AsV and Asll respectively. Results of incubation study carried out in basal salt minimal media (BSMY) containing 25 mg l-1 of AsV and Aslll separately showed that the isolates could accumulate 1.03 - 6.41 mg I-1 of AsV and 2.0 - 7.6 mg l-1 of Aslll from the media. The bacterial isolate AGH-21 showed highest As accumulating capacity both for AsV (25.64%) and Aslll (30.4%) under laboratory conditions. The isolates AGH-21 (NCBI accession no: HQ834295) showed highest sequence similarity (98%) with Bacillus sp. and could be used as a potential bioremediator in future to combat with arsenic toxicity.
authors.affiliation: Directorate of Research, Bidhan Chandra Krishi Viswavidyalaya, Kalyani-741 235, India. aparajita_micro@yahoo.co.in
authors.forename: Aparajita
authors.initials: A
authors.lastname: Majumder
authors.affiliation: Department ofMicrobiology, University of Calcutta, Kolkata -700 019, India.
authors.forename: Sagarmoy
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Directorate of Research, Bidhan Chandra Krishi Viswavidyalaya, Kalyani-741 235, India.
authors.forename: Niharendu
authors.initials: N
authors.lastname: Saha
authors.affiliation: Directorate of Research, Bidhan Chandra Krishi Viswavidyalaya, Kalyani-741 235, India.
authors.forename: S C
authors.initials: SC
authors.lastname: Kole
authors.affiliation: Directorate of Research, Bidhan Chandra Krishi Viswavidyalaya, Kalyani-741 235, India.
authors.forename: Supradip
authors.initials: S
authors.lastname: Sarkar
mesh_terms: D001151:Arsenic / Q000378:metabolism*; D001419:Bacteria / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D052918:Environmental Restoration and Remediation / Q000379:methods*; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D001151:Arsenic