title: Evaluation of the spoilage potential of bacteria isolated from spoiled cooked whole tropical shrimp (Penaeus vannamei) stored under modified atmosphere packaging.
abstract: The spoilage potential of isolates belonging to five bacterial groups/species (Shewanella baltica, Carnobacterium maltaromaticum, Aeromonas salmonicida, Vibrio sp., "other Gamma-Proteobacteria" [containing one strain of Pseudoalteromonas sp. and one strain of Psychrobacter sp.]) isolated from spoiled cooked and whole tropical shrimp stored under modified atmosphere packaging (MAP) was evaluated by inoculation into ionized cooked and peeled tropical shrimp followed by storage for 32 days at 8 °C. Microbial growth and sensory changes were monitored during the storage period. The major spoilage bacterial isolate groups were C. maltaromaticum and S. baltica. In order to characterize their spoilage potential further and to study the effect of their interactions, each of these two specific spoilage organisms (SSO) and one mixed-culture, C. maltaromaticum/S. baltica, were tested using a combination of complementary methods: molecular (PCR-TTGE), sensory, chemical, and conventional microbiological analyses. It was concluded that, in the mixed-culture-inoculated samples, both species groups imposed their spoilage characteristics.
authors.affiliation: LUNAM Université, ONIRIS, Université Nantes, UMR1014 Secalim, Nantes F-44307, France; INRA, Nantes F-44307, France; Ifremer, Laboratoire Science et Technologie de la Biomasse Marine, BP 21105, 44311 Nantes Cedex 3, France.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Macé
authors.affiliation: Ifremer, Laboratoire Science et Technologie de la Biomasse Marine, BP 21105, 44311 Nantes Cedex 3, France.
authors.forename: Mireille
authors.initials: M
authors.lastname: Cardinal
authors.affiliation: LUNAM Université, ONIRIS, Université Nantes, UMR1014 Secalim, Nantes F-44307, France; INRA, Nantes F-44307, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Jaffrès
authors.affiliation: Ifremer, Laboratoire Science et Technologie de la Biomasse Marine, BP 21105, 44311 Nantes Cedex 3, France.
authors.forename: Josiane
authors.initials: J
authors.lastname: Cornet
authors.affiliation: LUNAM Université, ONIRIS, Université Nantes, UMR 6144 GEPEA, Flavor Unit, F-44307, France; CNRS, Nantes F-44307, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Lalanne
authors.affiliation: Ifremer, Laboratoire Science et Technologie de la Biomasse Marine, BP 21105, 44311 Nantes Cedex 3, France.
authors.forename: Frédérique
authors.initials: F
authors.lastname: Chevalier
authors.affiliation: LUNAM Université, ONIRIS, Université Nantes, UMR 6144 GEPEA, Flavor Unit, F-44307, France; CNRS, Nantes F-44307, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Sérot
authors.affiliation: LUNAM Université, ONIRIS, Université Nantes, UMR1014 Secalim, Nantes F-44307, France; INRA, Nantes F-44307, France.
authors.forename: Marie-France
authors.initials: MF
authors.lastname: Pilet
authors.affiliation: LUNAM Université, ONIRIS, Université Nantes, UMR1014 Secalim, Nantes F-44307, France; INRA, Nantes F-44307, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Dousset
authors.affiliation: Ifremer, Laboratoire Science et Technologie de la Biomasse Marine, BP 21105, 44311 Nantes Cedex 3, France. Electronic address: jean.jacques.joffraud@ifremer.fr.
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Joffraud
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D003296:Cooking; D018857:Food Packaging / Q000379:methods*; D061353:Food Storage; D006801:Humans; D033561:Penaeidae / Q000737:chemistry / Q000382:microbiology*; D012758:Shellfish / Q000032:analysis / Q000382:microbiology*; D013649:Taste
keywords: Bacterial interaction; Cooked tropical shrimp; Specific spoilage organism; Spoilage potential; Volatile compounds