title: Family-based analysis of adiponectin gene polymorphisms in Chinese Han polycystic ovary syndrome.
abstract: OBJECTIVE
  To study the association between two single-nucleotide polymorphisms (SNPs) variants (rs2241766 and rs1501299) of the adiponectin gene (ADIPOQ) and polycystic ovary syndrome (PCOS) in PCOS family trios.
  
  
  METHODS
  Family-based study.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  A total of 224 unrelated PCOS probands, their biologic parents, and 204 control subjects.
  
  
  METHODS
  Anthropometric, hormonal, and metabolic assessment; genotype detection.
  
  
  METHODS
  Basic endocrine, serum fasting glucose (FG), fasting insulin (FINS), total cholesterol (TC), triglycerides (TG), and low-density lipoprotein (LDL) and high-density lipoprotein (HDL) cholesterol levels were measured. The transmission disequilibrium test (TDT) was used to analyze the association between two SNPs of ADIPOQ and PCOS.
  
  
  RESULTS
  A significant positive association was detected between SNP rs1501299 and PCOS. Compared with AA genotype, AC and CC genotypes had increased risk for PCOS, and the difference remained significant after adjustment for age, body mass index, and testosterone (T). The levels of T, TG, HDL cholesterol, and FINS were significantly higher in obese PCOS patients. However, we failed to find significant overtransmission of the other SNP, rs2241766, from parents to PCOS offspring. The genotype frequencies of rs2241766 and rs1501299 were not different in obese and lean PCOS patients and their parents.
  
  
  CONCLUSIONS
  TDT confirms that SNP rs1501299 in the ADIPOQ is significantly associated with the risk of PCOS in the Chinese Han population. The present data may provide a basis for further studies of the role of the ADIPOQ in the etiology of PCOS.
authors.affiliation: Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan, People's Republic of China; Center for Reproductive Medicine, Central Hospital, Taian, People's Republic of China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan, People's Republic of China.
authors.forename: Daimin
authors.initials: D
authors.lastname: Wei
authors.affiliation: Taishan Medical College, Taian, People's Republic of China.
authors.forename: Xianchang
authors.initials: X
authors.lastname: Sun
authors.affiliation: Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Center for Reproductive Medicine, Central Hospital, Taian, People's Republic of China.
authors.forename: Xinyan
authors.initials: X
authors.lastname: Yu
authors.affiliation: Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan, People's Republic of China. Electronic address: shiyuhua2003@126.com.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan, People's Republic of China.
authors.forename: Zi-Jiang
authors.initials: ZJ
authors.lastname: Chen
mesh_terms: D052242:Adiponectin / Q000235:genetics*; D000328:Adult; D044466:Asian People / Q000235:genetics*; D005190:Family; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D011085:Polycystic Ovary Syndrome / Q000175:diagnosis* / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D055815:Young Adult
chemical_list: C499918:ADIPOQ protein, human; D052242:Adiponectin
keywords: ADIPOQ; Polycystic ovary syndrome; single-nucleotide polymorphism; transmission disequilibrium test