title: Lactotransferrin-Cre reporter mice trace neutrophils, monocytes/macrophages and distinct subtypes of dendritic cells.
abstract: Considerable effort has been expended to identify genes that account for myeloid lineage commitment and development. However, currently available non-invasive mouse models utilize myeloid-specific reporters that are significantly expressed in hematopoietic stem cells as well as lymphoid compartments. Here, we describe a myeloid-specific marker that is not shared by any other lineage. We show that lactotransferrin mRNA is expressed by Gr-1(+)/CD11b(+) cells in the bone marrow, as opposed to hematopoietic stem cells or any peripheral cell population. To follow the progeny of lactotransferrin-expressing bone marrow cells, we generated a mouse model in which a reporter gene is irreversibly activated from the lactotransferrin-promoter. We found that lactotransferrin-reporter labels a majority of neutrophils, monocytes, macrophages and distinct subtypes of dendritic cells, while excluding T, B, natural killer cells, interferon-producing killer dendritic cells, plasmacytoid dendritic cells, erythrocytes and eosinophils. Lactotransferrin-reporter(-) bone marrow cells retain lymphoid, erythroid and long-term repopulating potential, while lactotransferrin-reporter(+) bone marrow cells confer only myeloid, but not lymphoid potential. We conclude that lactotransferrin represents a late stage differentiation marker of neutrophils, macrophages and distinct subtypes of dendritic cells.
authors.affiliation: Institute of Animal Breeding and Genetics, University of Veterinary Medicine Vienna, Vienna, Austria Research Institute of Molecular Pathology (I.M.P.), Vienna, Austria boris.kovacic@meduniwien.ac.at mathias.mueller@vetmeduni.ac.at.
authors.forename: Boris
authors.initials: B
authors.lastname: Kovacic
authors.affiliation: Institute of Pharmacology and Toxicology, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Andrea
authors.initials: A
authors.lastname: Hoelbl-Kovacic
authors.affiliation: Institute of Animal Breeding and Genetics, University of Veterinary Medicine Vienna, Vienna, Austria.
authors.forename: Katrin M
authors.initials: KM
authors.lastname: Fischhuber
authors.affiliation: Institute of Animal Breeding and Genetics, University of Veterinary Medicine Vienna, Vienna, Austria.
authors.forename: Nicole R
authors.initials: NR
authors.lastname: Leitner
authors.affiliation: Institute of Pharmacology and Toxicology, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Gotthardt
authors.affiliation: Ludwig Boltzmann Institute for Cancer Research (LBI-CR), Vienna, Austria Institute of Pharmacology, Center of Physiology and Pharmacology, Comprehensive Cancer Center, Medical University of Vienna, Austria.
authors.forename: Emilio
authors.initials: E
authors.lastname: Casanova
authors.affiliation: Institute of Pharmacology and Toxicology, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Veronika
authors.initials: V
authors.lastname: Sexl
authors.affiliation: Institute of Animal Breeding and Genetics, University of Veterinary Medicine Vienna, Vienna, Austria boris.kovacic@meduniwien.ac.at mathias.mueller@vetmeduni.ac.at.
authors.forename: Mathias
authors.initials: M
authors.lastname: Müller
mesh_terms: D000818:Animals; D039481:CD11b Antigen / Q000378:metabolism; D058948:Cell Tracking; D003713:Dendritic Cells / Q000378:metabolism*; D041905:Erythroid Cells / Q000378:metabolism; D015870:Gene Expression; D023061:Gene Order; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics; D007781:Lactoferrin / Q000235:genetics* / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D009000:Monocytes / Q000378:metabolism*; D022423:Myeloid Cells / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism*; D009928:Organ Specificity / Q000235:genetics; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D019707:Receptors, Chemokine / Q000378:metabolism
chemical_list: D039481:CD11b Antigen; C503133:Gr-1 protein, mouse; C552902:Ltf protein, mouse; D012333:RNA, Messenger; D019707:Receptors, Chemokine; D007781:Lactoferrin