title: A simplified chromatographic approach to purify commercially available bovine submaxillary mucins (BSM).
abstract: In this study, a simple purification protocol is developed to reduce the bovine serum albumin (BSA) content in commercially available bovine submaxillary mucin (BSM). This involved purification of the BSM by one-column anion-exchange chromatography protocol resulting in BSM with greatly reduced BSA content and homogeneously distributed size, and in a high yield of ∼43% from BSM as received from the manufacturer. The purity and composition of commercially acquired BSM were assessed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and mass spectrometry, which verified that BSA is the most abundant nonmucinous protein component. The purification effect was evident from a significantly altered circular dichroism (CD) spectrum of BSM after anion-exchange chromatography.
authors.affiliation: a Department of Mechanical Engineering , Technical University of Denmark , Kgs. Lyngby , Denmark.
authors.forename: Jan Busk
authors.initials: JB
authors.lastname: Madsen
authors.forename: Kirsi I
authors.initials: KI
authors.lastname: Pakkanen
authors.forename: Lars
authors.initials: L
authors.lastname: Duelund
authors.forename: Birte
authors.initials: B
authors.lastname: Svensson
authors.forename: Maher Abou
authors.initials: MA
authors.lastname: Hachem
authors.forename: Seunghwan
authors.initials: S
authors.lastname: Lee
mesh_terms: D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange / Q000379:methods*; D002855:Chromatography, Thin Layer; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D009077:Mucins / Q000737:chemistry / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: C115439:BSM1 protein, Bos taurus; D009077:Mucins; D012710:Serum Albumin, Bovine
keywords: anion-exchange chromatography (AEC); bovine serum albumin (BSA); bovine submaxillary mucin (BSM); mucin; purification