title: Mode of action of diterpene and characterization of related metabolites from the soft coral, Xenia elongata.
abstract: Chemical and biological investigation of the cultured marine soft coral Xenia elongata led to the isolation of two new diterpenes (2, 3). Their structures were elucidated using a combination of NMR and mass spectrometry. Biological evaluations and assessments were determined using the specific apoptosis induction assay based on genetically engineered mammalian cell line D3 deficient in Bak and Bax and derived from a mouse epithelial cell. The diterpenes induce apoptosis in low micromolar concentrations. The results indicate that the previously isolated compound (1) affects cell in a manner similar to that of HSP90 and HDAC inhibitors and in a manner opposite of PI3 kinase/mTOR inhibitors. Compound (3) inhibits selectively HDAC6 in high micromolar concentrations.
authors.affiliation: Center for Marine Biotechnology, Institute of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA. andriane@marine.rutgers.edu.
authors.forename: Eric H
authors.initials: EH
authors.lastname: Andrianasolo
authors.affiliation: Center for Marine Biotechnology, Institute of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA. haramaty@marine.rutgers.edu.
authors.forename: Liti
authors.initials: L
authors.lastname: Haramaty
authors.affiliation: The Cancer Institute of New Jersey, 195 Little Albany Street, New Brunswick, NJ 08903, USA. epwhite@cinj.rutgers.edu.
authors.forename: Eileen
authors.initials: E
authors.lastname: White
authors.affiliation: Center for Marine Biotechnology, Institute of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA. rlutz@imcs.marine.rutgers.edu.
authors.forename: Richard
authors.initials: R
authors.lastname: Lutz
authors.affiliation: Center for Marine Biotechnology, Institute of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA. falko@imcs.marine.rutgers.edu.
authors.forename: Paul
authors.initials: P
authors.lastname: Falkowski
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D005818:Genetic Engineering; D000073864:Histone Deacetylase 6; D006655:Histone Deacetylases / Q000187:drug effects / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D051379:Mice
chemical_list: D004224:Diterpenes; C499033:Hdac6 protein, mouse; D000073864:Histone Deacetylase 6; D006655:Histone Deacetylases