title: Two new compounds and anti-complementary constituents from Amomum tsao-ko.
abstract: Two new compounds, (2R,3R,4R)-3',5'-dimethoxy-3,4,7,4'-tetrahydroxy-flavan (1) and 2-(4-hydroxy-3-methoxybenzoyl)-4-methoxy-benzaldehyde (2), together with 35 known phenolic compounds were obtained from the fruits of Amomum tsao-ko. Structures of the new compounds were elucidated on the basis of spectroscopic means, including 2D NMR, a n d high-resolution M S analysis. The isolated compounds were tested in vitro for t heircomplement-inhibitory properties against the classical pathway (CP) and alternative pathway (AP). The results showed that 14 compounds exhibited anti-complementary activities against the CP and AP with CH50 values of 0.42 - 4.43 mM and AP50 values of 0.53 -1.51 mM. Preliminary mechanism studies showed that 1,7-bis(4-hydroxyphenyl)-4(E)-hepten-3-one (8) blocked C1q, C2, C3, C4, C5 and C9 components of the complement system, and hydroquinone (15) acted on C1q, C2, C3, C5 and C9 components.
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 201203, People's Republic of China.
authors.forename: Jiahong
authors.initials: J
authors.lastname: Jin
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 201203, People's Republic of China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 201203, People's Republic of China.
authors.forename: Daofeng
authors.initials: D
authors.lastname: Chen
mesh_terms: D035801:Amomum / Q000737:chemistry*; D000818:Animals; D001547:Benzaldehydes / Q000737:chemistry / Q000302:isolation & purification*; D051056:Complement Inactivating Agents / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D005638:Fruit / Q000737:chemistry; D006801:Humans; D008297:Male; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry; D011817:Rabbits; D012756:Sheep
chemical_list: C587186:(2R,3R,4R)-3',5'-dimethoxy-3,4,7,4'-tetrahydroxy-flavan; C587187:2-(4-hydroxy-3-methoxybenzoyl)-4-methoxy-benzaldehyde; D001547:Benzaldehydes; D051056:Complement Inactivating Agents; D004365:Drugs, Chinese Herbal; D005419:Flavonoids