title: SHCBP1L, a conserved protein in mammals, is predominantly expressed in male germ cells and maintains spindle stability during meiosis in testis.
abstract: Male subfertility due to falling sperm counts has become an increasing problem over a short timescale (50-70 years). Recently, bioinformatics analysis of the human testis proteome has revealed the existence of human-testicular-predominantly-expressed-proteins, which are highly associated with spermatogenesis, although the functions of many of these proteins are still unknown. To understand the function of one of these proteins, SHCBP1L (1700012A16RIKEN), a knockout mouse was produced in which this gene was removed. Using this model, we showed that SHCBP1L binds to another protein, HSPA2, and maintains stability of the spindle. We showed that this complex was not present in knockout mice and that an abnormal number of spermatocytes were held in the early stages of meiosis. Many of these cells were undergoing programmed cell-death, or apoptosis, which is highly unusual for cells during the early stages of meiosis. We also found that proteins very similar to SHCBP1L exist in many other mammals. This led us to propose that SHCBP1L plays an important role in spermatogenesis in mammals.
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, Jiangsu, China.
authors.forename: Mingxi
authors.initials: M
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, Jiangsu, China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, Jiangsu, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Bi
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, Jiangsu, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Qi
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, Jiangsu, China.
authors.forename: Xuejiang
authors.initials: X
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, Jiangsu, China zhouzm@njmu.edu.cn wanglei@njmu.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, Jiangsu, China zhouzm@njmu.edu.cn wanglei@njmu.edu.cn.
authors.forename: Zuomin
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, Jiangsu, China.
authors.forename: Jiahao
authors.initials: J
authors.lastname: Sha
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D002453:Cell Cycle / Q000235:genetics; D017124:Conserved Sequence; D015870:Gene Expression; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D008540:Meiosis; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D055765:Shc Signaling Adaptor Proteins / Q000235:genetics* / Q000378:metabolism; D013076:Sperm Count; D013090:Spermatocytes / Q000166:cytology / Q000378:metabolism*; D013091:Spermatogenesis / Q000235:genetics; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism*; D008941:Spindle Apparatus / Q000378:metabolism* / Q000648:ultrastructure; D013737:Testis / Q000166:cytology / Q000378:metabolism*
chemical_list: D018840:HSP70 Heat-Shock Proteins; C490434:Hspa2 protein, mouse; C000594057:SHCBP1L protein, human; D055765:Shc Signaling Adaptor Proteins
keywords: HSPA2; SHCBP1L; meiosis; spermatogenesis; spindle