title: Molecular epidemiology of Aleutian mink disease virus in China.
abstract: Aleutian mink disease (mink plasmacytosis) is a very severe immune-complex-mediated disease affecting minks. It is caused by the Aleutian mink disease virus (AMDV). To obtain a better understanding of the molecular epidemiology of AMDV in China, a total of 420 samples were collected from mink farms in five major mink-farming provinces in China. After testing serum antibodies using counterimmunoelectrophoresis (CIEP), 23 of the 340 positive samples were randomly selected and analyzed. The full length of the major structural protein gene (VP2) from all the samples was amplified and sequenced. The sequences in the twenty-three samples from 5 farms in 5 provinces were phylogenetically analyzed, and eleven were found to have homologous sequences in GenBank. A rooted phylogenetic tree was constructed using the unweighted pair-group method with arithmetic (UPGMA) method. Phylogenetic analysis showed that the AMDV strains formed five groups (I-VI), and four of them contained Chinese strains. The tree showed that the two AMVD lineages had been introduced to China independently. Over 70% of the Chinese isolates were classified into two groups, all of which contained Chinese strains. The results of the analysis suggested that the distribution of the AMDV strains was not based on geographical origin, and both indigenous AMDV and imported AMDV were prevalent in the primary mink production areas in China.
authors.affiliation: Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, China.
authors.forename: Zhenjun
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Hu
authors.affiliation: Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, China.
authors.forename: Hailing
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Bai
authors.affiliation: Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, China. Electronic address: kexin79@foxmail.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, China. Electronic address: yanxijungmp@163.com.
authors.forename: Xijun
authors.initials: X
authors.lastname: Yan
mesh_terms: D000453:Aleutian Mink Disease / Q000453:epidemiology* / Q000821:virology*; D000454:Aleutian Mink Disease Virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D036022:Capsid Proteins / Q000235:genetics; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D003378:Counterimmunoelectrophoresis; D005838:Genotype; D008907:Mink; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; C442555:VP2 protein, Aleutian mink disease virus
keywords: Aleutian mink disease virus; Molecular epidemiology; Phylogenetic analysis; VP2