title: Effect of the IkBα mutant gene delivery to mesenchymal stem cells on rat chronic pancreatitis.
abstract: This study aimed to investigate the effect of inhibitors of the NF-kΒ alpha mutant gene (IkBaM) delivery to mensenchymal stem cells (MSCs) on rat chronic pancreatitis (CP). A total of 120 Sprague-Dawley rats were randomly divided into 6 groups of 20: Group A was injected with sterile saline solution, Group B was injected with allogenic MSCs, Group C1 was injected with allogenic IkBαM-MSCs cultured in vitro 4 h before CP modeling, Group C2 was injected with allogenic IkBαM-MSCs cultured in vitro during CP modeling, Group C3 was cultured with allogenic IkBαM-MSCs cultured in vitro 4 h after CP modeling, and Group D was injected with rAAV2-MSCs. Cytokine levels of ICAM-1, CTGF, IL-1, IL-6, IL-8, TNF-α, TIMP-1, TIMP-2, IL-10, FN, MMP-1, MMP-2, MMP-3, and MMP-9 were examined. The results indicated that allogenic IκBαM-MSCs could reduce pro-inflammatory cytokine levels and increase anti-inflammatory cytokine levels in CP. The allogenic IkBαM-MSCs reduced the activation and promoted the apoptosis of pancreatic stellate cells in the rat model of CP. IkBαM-MSCs influenced the proliferation and apoptosis of pancreatic stellate cells by regulating the activation of the PPAR, MAPK, mTOR, TGF-β, NOD-like receptor, Notch, WNT, TGF-β1-SMAD-2/3, and P53 signal transduction pathways.
authors.affiliation: Department of Hepatobiliary Pancreatic Surgery, Henan People's Hospital, Zhengzhou, Henan Province, China.
authors.forename: T
authors.initials: T
authors.lastname: Qin
authors.affiliation: Department of Hepatobiliary Pancreatic Surgery, Henan People's Hospital, Zhengzhou, Henan Province, China.
authors.forename: C J
authors.initials: CJ
authors.lastname: Liu
authors.affiliation: Department of Hepatobiliary Pancreatic Surgery, Henan People's Hospital, Zhengzhou, Henan Province, China hongweizhangcn@163.com.
authors.forename: H W
authors.initials: HW
authors.lastname: Zhang
authors.affiliation: Department of Infection Disease, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China.
authors.forename: Y F
authors.initials: YF
authors.lastname: Pan
authors.affiliation: Department of Hepatobiliary Pancreatic Surgery, Henan People's Hospital, Zhengzhou, Henan Province, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Tang
authors.affiliation: Department of Hepatobiliary Pancreatic Surgery, Henan People's Hospital, Zhengzhou, Henan Province, China.
authors.forename: J K
authors.initials: JK
authors.lastname: Liu
authors.affiliation: Department of Hepatobiliary Pancreatic Surgery, Henan People's Hospital, Zhengzhou, Henan Province, China.
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Wang
authors.affiliation: Department of Hepatobiliary Pancreatic Surgery, Henan People's Hospital, Zhengzhou, Henan Province, China.
authors.forename: M X
authors.initials: MX
authors.lastname: Hu
authors.affiliation: Department of Hepatobiliary Pancreatic Surgery, Henan People's Hospital, Zhengzhou, Henan Province, China.
authors.forename: F
authors.initials: F
authors.lastname: Xue
mesh_terms: D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D000229:Dependovirus / Q000235:genetics / Q000378:metabolism; D015316:Genetic Therapy; D020672:I-kappa B Proteins / Q000235:genetics / Q000378:metabolism*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D009154:Mutation; D000072000:NF-KappaB Inhibitor alpha; D050500:Pancreatitis, Chronic / Q000628:therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction
chemical_list: D016207:Cytokines; D020672:I-kappa B Proteins; C000606206:Nfkbia protein, rat; D000072000:NF-KappaB Inhibitor alpha