title: Fluoxetine pretreatment promotes neuronal survival and maturation after auditory fear conditioning in the rat amygdala.
abstract: The amygdala is a critical brain region for auditory fear conditioning, which is a stressful condition for experimental rats. Adult neurogenesis in the dentate gyrus (DG) of the hippocampus, known to be sensitive to behavioral stress and treatment of the antidepressant fluoxetine (FLX), is involved in the formation of hippocampus-dependent memories. Here, we investigated whether neurogenesis also occurs in the amygdala and contributes to auditory fear memory. In rats showing persistent auditory fear memory following fear conditioning, we found that the survival of new-born cells and the number of new-born cells that differentiated into mature neurons labeled by BrdU and NeuN decreased in the amygdala, but the number of cells that developed into astrocytes labeled by BrdU and GFAP increased. Chronic pretreatment with FLX partially rescued the reduction in neurogenesis in the amygdala and slightly suppressed the maintenance of the long-lasting auditory fear memory 30 days after the fear conditioning. The present results suggest that adult neurogenesis in the amygdala is sensitive to antidepressant treatment and may weaken long-lasting auditory fear memory.
authors.affiliation: Mental Health Institute, The Second Xiangya Hospital, and Key Lab of Psychiatry and Mental Health of Hunan Province, Central South University, Changsha, China ; Department of neuropsychopathy, clinical medical school, Dali University, Dali, China.
authors.forename: Lizhu
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of Anatomy and Neurobiology, School of Basic Medical Sciences, Central South University, Changsha, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Liu
authors.affiliation: The Third Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Tong
authors.affiliation: Key Lab of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming, China.
authors.forename: Rongrong
authors.initials: R
authors.lastname: Mao
authors.affiliation: Department of Anatomy and Neurobiology, School of Basic Medical Sciences, Central South University, Changsha, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Anatomy and Neurobiology, School of Basic Medical Sciences, Central South University, Changsha, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Anatomy and Neurobiology, School of Basic Medical Sciences, Central South University, Changsha, China.
authors.forename: Jufang
authors.initials: J
authors.lastname: Huang
authors.affiliation: Mental Health Institute, The Second Xiangya Hospital, and Key Lab of Psychiatry and Mental Health of Hunan Province, Central South University, Changsha, China.
authors.forename: Lingjiang
authors.initials: L
authors.lastname: Li
mesh_terms: D000161:Acoustic Stimulation; D000679:Amygdala / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D018687:Antidepressive Agents, Second-Generation / Q000494:pharmacology*; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D001973:Bromodeoxyuridine; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003214:Conditioning, Classical / Q000187:drug effects / Q000502:physiology; D005239:Fear / Q000187:drug effects* / Q000523:psychology; D005473:Fluoxetine / Q000494:pharmacology*; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D008297:Male; D055495:Neurogenesis / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018687:Antidepressive Agents, Second-Generation; D005473:Fluoxetine; D001973:Bromodeoxyuridine