title: 3-nitrotyrosine levels in dichlorvos-induced neurotoxicity.
abstract: The aim of this study was to evaluate dichlorvos toxicity in terms of nitro-oxidative stress by determining 3-nitrotyrosine (3-NT) levels in the fore, mid, and hindbrain regions in acutely exposed rats. Male Sprague- Dawley rats were randomly allocated to three groups of eight. Group 1 was administered a single intraperitoneal dichlorvos dose of 1.8 mg kg ⁻¹(0.1xLD₅₀) and group 2 a dose of 9 mg kg⁻¹ (0.5xLD₅₀). The control group received 0.5 mL saline solution via the same route. 3-NT and tyrosine (TYR) levels were measured using high performance liquid chromatography with a photodiode array detector (HPLC-PDA) and expressed as a ratio of 3-NT to TYR. The 3-NT/1000 TYR ratios increased significantly in the fore-, mid- and hindbrains of the exposed groups compared to control (p<0.01). In the forebrain, the increase was also significant between the treated groups. Our study has confirmed that acute exposure to dichlorvos leads to nitro-oxidative stress in the brain and that 3-NT may play a role in the mechanism of dichlorvos neurotoxicity.
authors.forename: Dilek
authors.initials: D
authors.lastname: Guvenç
authors.forename: Abdurrahman
authors.initials: A
authors.lastname: Aksoy
authors.forename: Yavuz
authors.initials: Y
authors.lastname: Kursad
authors.forename: Enes
authors.initials: E
authors.lastname: Atmaca
authors.forename: Oguzhan
authors.initials: O
authors.lastname: Yavuz
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism*; D004006:Dichlorvos / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity*; D007928:Lethal Dose 50; D008297:Male; D020258:Neurotoxicity Syndromes / Q000209:etiology* / Q000378:metabolism*; D018384:Oxidative Stress; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D007306:Insecticides; C002744:3-nitrotyrosine; D014443:Tyrosine; D004006:Dichlorvos