title: Identification of secreted virulence factors of Chromobacterium violaceum.
abstract: Chromobacterium violaceum, a component of tropical soil microbiota, is an opportunistic pathogenic bacterium that can infect humans and other animals. In addition to identifying a large number of genes that demonstrate the vast biotechnological potential of this bacterium, genome sequencing revealed several virulence factors, including different cytolysins, which can be related to its pathogenicity. Here we confirmed these predictions from genomic analyses by identifying, through mass spectrometry, proteins present in the culture supernatant of C. violaceum that may constitute secreted virulence factors. Among them, we identified a secreted collagenase and the product of a gene with sequence similarity to previously characterized bacterial porins.
authors.affiliation: Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Thiago
authors.initials: T
authors.lastname: Castro-Gomes
authors.forename: Mariana S
authors.initials: MS
authors.lastname: Cardoso
authors.forename: Wanderson D
authors.initials: WD
authors.lastname: DaRocha
authors.forename: Letícia A
authors.initials: LA
authors.lastname: Laibida
authors.forename: Andréa M A
authors.initials: AM
authors.lastname: Nascimento
authors.forename: Luciana W
authors.initials: LW
authors.lastname: Zuccherato
authors.forename: Maria Fátima
authors.initials: MF
authors.lastname: Horta
authors.forename: Marcelo P
authors.initials: MP
authors.lastname: Bemquerer
authors.forename: Santuza M R
authors.initials: SM
authors.lastname: Teixeira
mesh_terms: D000818:Animals; D002861:Chromobacterium / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry; D012988:Soil Microbiology; D014329:Tropical Climate; D037521:Virulence Factors / Q000032:analysis* / Q000235:genetics
chemical_list: D003470:Culture Media; D037521:Virulence Factors