title: Chronically sun-damaged melanomas express low levels of nuclear glutathione-S-transferase-π: an epidemiological and clinicopathological study in Italy.
abstract: The detoxifying enzyme glutathione-s-transferase pi (GST-π) is present in keratinocytes and melanocytes and exerts a protective role against tumour progression. Melanomas close to melanocytic naevus remnants occur less frequently on sun-exposed areas, whereas solar dermal elastosis, hallmark of chronic sun-damage, characterise melanomas on sun-exposed skin. We evaluated the expression of GST-π in 113 melanomas associated to melanocytic naevus remnants or to solar dermal elastosis, classified according to clinical characteristics, history of sun exposure, histological subtypes and AJCC staging. Chronically sun-damaged melanomas, identified by moderate-severe solar dermal elastosis, showed a lower nuclear GST-π expression and a higher thickness than those related to melanocytic naevus remnants (p < 0.03). Multivariate logistic regression analysis demonstrated that male gender and chronic sun-exposure are independent risk factors significantly associated to melanomas localised on the trunk (OR = 3.36, 95% CI: 1.31-8.65; OR = 5.97, 95% CI: 1.71-20.87). If confirmed on a larger series, lower expression of nuclear GST-π in melanoma cells could represent a possible marker of chronically sun-damaged melanoma pathogenesis.
authors.affiliation: Department of Dermatology, University of Rome.
authors.forename: Elena
authors.initials: E
authors.lastname: Campione
authors.forename: Emanuela
authors.initials: E
authors.lastname: Medda
authors.forename: Evelin J
authors.initials: EJ
authors.lastname: Paternò
authors.forename: Laura
authors.initials: L
authors.lastname: Diluvio
authors.forename: Ilaria
authors.initials: I
authors.lastname: Ricozzi
authors.forename: Isabella
authors.initials: I
authors.lastname: Carboni
authors.forename: Gaetana
authors.initials: G
authors.lastname: Costanza
authors.forename: Piero
authors.initials: P
authors.lastname: Rossi
authors.forename: Cristina
authors.initials: C
authors.lastname: Rapanotti
authors.forename: Alessandro
authors.initials: A
authors.lastname: Di Stefani
authors.forename: Sergio
authors.initials: S
authors.lastname: Chimenti
authors.forename: Luca
authors.initials: L
authors.lastname: Bianchi
authors.forename: Augusto
authors.initials: A
authors.lastname: Orlandi
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D001706:Biopsy; D016009:Chi-Square Distribution; D015536:Down-Regulation; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D051549:Glutathione S-Transferase pi / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D007558:Italy / Q000453:epidemiology; D016015:Logistic Models; D008297:Male; D008545:Melanoma / Q000201:enzymology* / Q000453:epidemiology* / Q000473:pathology; D008875:Middle Aged; D015999:Multivariate Analysis; D009367:Neoplasm Staging; D009381:Neoplasms, Radiation-Induced / Q000201:enzymology* / Q000453:epidemiology* / Q000473:pathology; D009508:Nevus, Pigmented / Q000201:enzymology* / Q000453:epidemiology* / Q000473:pathology; D016017:Odds Ratio; D012307:Risk Factors; D012737:Sex Factors; D013472:Sunlight / Q000009:adverse effects*; D013997:Time Factors
chemical_list: D014408:Biomarkers, Tumor; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi