title: The facile generation of two-dimensional stiffness maps in durotactic cell platforms through thickness projections of three-dimensional submerged topography.
abstract: An innovative platform that aims to facilitate studies of how adherent cells migrate in response to rigidity gradients or durotaxis has been developed. Soft polyacrylamide gel-based cell culture scaffolds are used to fabricate flat surfaces containing elasticity gradients through changes in the underlying patterned features. Moreover, this inert gel surface supports long-term cell viability and offers a tunable stiffness. By manipulating the thickness of the gel substrate through the embedded patterns, this system is also capable of directing collective cell patterning.
authors.affiliation: Biological and Soft Systems, Cavendish Laboratory, University of Cambridge, Cambridge, United Kingdom.
authors.forename: C H R
authors.initials: CH
authors.lastname: Kuo
authors.affiliation: Biological and Soft Systems, Cavendish Laboratory, University of Cambridge, Cambridge, United Kingdom; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
authors.forename: J
authors.initials: J
authors.lastname: Láng
authors.affiliation: Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary; Institute for Integrated Cell-Material Sciences, Kyoto University, Kyoto, Japan.
authors.forename: O
authors.initials: O
authors.lastname: Láng
authors.affiliation: Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
authors.forename: L
authors.initials: L
authors.lastname: Kőhidai
authors.affiliation: Biological and Soft Systems, Cavendish Laboratory, University of Cambridge, Cambridge, United Kingdom; Institute for Integrated Cell-Material Sciences, Kyoto University, Kyoto, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Sivaniah
mesh_terms: D016475:3T3 Cells; D000180:Acrylic Resins; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002450:Cell Communication / Q000502:physiology*; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D020099:Coated Materials, Biocompatible; D004548:Elasticity; D005109:Extracellular Matrix; D016326:Extracellular Matrix Proteins; D005347:Fibroblasts / Q000502:physiology; D056945:Hep G2 Cells; D006801:Humans; D061986:MCF-7 Cells; D051379:Mice; D013314:Stress, Mechanical; D013499:Surface Properties
chemical_list: D000180:Acrylic Resins; D020099:Coated Materials, Biocompatible; D016326:Extracellular Matrix Proteins; C016680:polyacrylamide gels
keywords: Cell migration; Durotaxis; Mechanosensing; Scaffolds