title: The proteome of the presynaptic active zone from mouse brain.
abstract: Neurotransmitter release as well as the structural and functional dynamics of the presynaptic active zone is controlled by proteinaceous components. Here we describe for the first time an experimental approach for the isolation of the presynaptic active zone from individual mouse brains, a prerequisite for understanding the functional inventory of the presynaptic protein network and for the later analysis of changes occurring in mutant mice. Using a monoclonal antibody against the ubiquitous synaptic vesicle protein SV2 we immunopurified synaptic vesicles docked to the presynaptic plasma membrane. Enrichment studies by means of Western blot analysis and mass spectrometry identified 485 proteins belonging to an impressive variety of functional categories. Our data suggest that presynaptic active zones represent focal hot spots that are not only involved in the regulation of neurotransmitter release but also in multiple structural and functional alterations the adult nerve terminal undergoes during neural activity in adult CNS. They furthermore open new avenues for characterizing alterations in the active zone proteome of mutant mice and their corresponding controls, including the various mouse models of neurological diseases.
authors.affiliation: Institute for Cell Biology and Neuroscience, Biologicum, Goethe-University, Frankfurt am Main, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Weingarten
authors.affiliation: Institute for Cell Biology and Neuroscience, Biologicum, Goethe-University, Frankfurt am Main, Germany.
authors.forename: Melanie
authors.initials: M
authors.lastname: Lassek
authors.affiliation: Institute of Pharmaceutical Chemistry, Cluster of Excellence "Macromolecular Complexes", Goethe-University, Frankfurt am Main, Germany.
authors.forename: Benjamin F
authors.initials: BF
authors.lastname: Mueller
authors.affiliation: Institute of Pharmaceutical Chemistry, Cluster of Excellence "Macromolecular Complexes", Goethe-University, Frankfurt am Main, Germany.
authors.forename: Marion
authors.initials: M
authors.lastname: Rohmer
authors.affiliation: Institute for Cell Biology and Neuroscience, Biologicum, Goethe-University, Frankfurt am Main, Germany.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Lunger
authors.affiliation: SunChrom Wissenschaftliche Geräte GmbH, Friedrichsdorf, Germany.
authors.forename: Dominic
authors.initials: D
authors.lastname: Baeumlisberger
authors.affiliation: Institute for Cell Biology and Neuroscience, Biologicum, Goethe-University, Frankfurt am Main, Germany.
authors.forename: Simone
authors.initials: S
authors.lastname: Dudek
authors.affiliation: Institute for Cell Biology and Neuroscience, Biologicum, Goethe-University, Frankfurt am Main, Germany.
authors.forename: Patricia
authors.initials: P
authors.lastname: Gogesch
authors.affiliation: Institute of Pharmaceutical Chemistry, Cluster of Excellence "Macromolecular Complexes", Goethe-University, Frankfurt am Main, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Karas
authors.affiliation: Institute for Cell Biology and Neuroscience, Biologicum, Goethe-University, Frankfurt am Main, Germany. Electronic address: Volknandt@bio.uni-frankfurt.de.
authors.forename: Walter
authors.initials: W
authors.lastname: Volknandt
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D017729:Presynaptic Terminals / Q000378:metabolism*; D020543:Proteome; D013570:Synaptic Membranes / Q000378:metabolism; D013572:Synaptic Vesicles / Q000378:metabolism
chemical_list: D020543:Proteome
keywords: Docked synaptic vesicles; Presynaptic active zone; Proteome