title: In vitro selection of RNA aptamers for a small-molecule dye.
abstract: Artificial riboswitches can be generated by functional coupling of an RNA aptamer for synthetic small molecule to an expression platform. RNA aptamers that can bind strongly and selectively to their target are feasible to be used for obtaining more potent artificial riboswitches. In this chapter, we describe tips and notes for in vitro selection of RNA aptamers targeting synthetic small molecules.
authors.affiliation: The Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Japan.
authors.forename: Asako
authors.initials: A
authors.lastname: Murata
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Sato
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry*; D058928:Riboswitch; D052156:SELEX Aptamer Technique / Q000379:methods*; D013050:Spectrometry, Fluorescence
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D058928:Riboswitch