title: Effect of feeding strategies on lipid production by Lipomyces starkeyi.
abstract: The aim of this study was to produce microbial oil from Lipomyces starkeyi DSM 70296 grown in hemicellulose hydrolysate (H-H). Glucose and xylose were used for batch, fed-batch, repeated fed-batch, and continuous cultures, and H-H was tested at continuous culture. The highest cell and lipid concentrations of 85.4 and 41.8g/L, respectively, were obtained using repeated fed-batch strategy. Continuous culture with dilution rate of 0.03h(-1) presented the highest overall cell (0.443g/g) and lipid yields (0.236g/g). At 0.06h(-1) were obtained the highest cell and lipid productivities. Continuous cultivation using H-H at 0.03h(-1) resulted in higher cell productivity than that obtained using glucose:xylose. Gas chromatography analysis of the esterified lipids indicated that the major constituents of this complex are palmitic acid, stearic acid, oleic acid, and linoleic acid with an estimated cetane number (approximately 61) similar to that of palm biodiesel, which is important for biofuel production.
authors.affiliation: Department of Chemical Processes, College of Chemical Engineering, UNICAMP (State University of Campinas), P.O. Box 6121, 13083-862 Campinas, SP, Brazil. Electronic address: eng.anschau@gmail.com.
authors.forename: Andréia
authors.initials: A
authors.lastname: Anschau
authors.affiliation: Department of Chemical Processes, College of Chemical Engineering, UNICAMP (State University of Campinas), P.O. Box 6121, 13083-862 Campinas, SP, Brazil. Electronic address: michellecax@gmail.com.
authors.forename: Michelle C A
authors.initials: MC
authors.lastname: Xavier
authors.affiliation: Department of Exact Sciences and Geoscience, College of Chemical Engineering, UNIFESP (Federal University of São Paulo), 09972-270 São Paulo, SP, Brazil. Electronic address: saartje.her@gmail.com.
authors.forename: Saartje
authors.initials: S
authors.lastname: Hernalsteens
authors.affiliation: Department of Chemical Processes, College of Chemical Engineering, UNICAMP (State University of Campinas), P.O. Box 6121, 13083-862 Campinas, SP, Brazil. Electronic address: franco@feq.unicamp.br.
authors.forename: Telma T
authors.initials: TT
authors.lastname: Franco
mesh_terms: D061249:Batch Cell Culture Techniques; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D049109:Cell Proliferation / Q000187:drug effects; D005227:Fatty Acids / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D008055:Lipids / Q000096:biosynthesis*; D055160:Lipomyces / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D014994:Xylose / Q000494:pharmacology
chemical_list: D005227:Fatty Acids; D008055:Lipids; D014994:Xylose; D005947:Glucose
keywords: Biodiesel; Continuous culture; Fed-batch; Oleaginous yeast; Sugarcane bagasse hydrolysate