title: [Bioactivity assay of bupleurum injection for inhibiting PGE2 release in vitro].
abstract: OBJECTIVE
  To establish the in vitro model of PGE2 released by hypothalamic neurocytes under rrIL-1beta in vitro interference, and investigate the correlation of the PGE2 content and the effect of the drug effect concentration in the model under the effect of Bupleurum injection.
  
  
  METHODS
  Hypothalamic neurocytes were cultured in vitro, and added with rrIL-1beta (40 microg x L(-1)) stimulation. Cell sap was collected at different time points. ELISA was adopted to determine the content of PGE2 in cell sap collected at different time points. Hypothalamic neurocytes were cultured in vitro, added with rrIL-1beta (40 microg x L(-1)) stimulation and then different concentrations of Bupleurum injection. The changes in the content of PGE2 in cell supernatant were detected by ELISA. An analysis was made on the linear relationship between the sample concentration and the inhibition rate of PGE2.
  
  
  RESULTS
  The rrIL-1 cells could stimulate in vitro cultured hypothalamic neurocytes to release PGE2 and reach the peak at 10 h. Bupleurum injection could significantly interfere the release of PGE2 in the in vitro model (P < 0.01, P < 0.05), with a certain linear relationship between the interference effect and the effect concentration of Bupleurum injection (r = 0.911, P < 0.01).
  
  
  CONCLUSIONS
  The rrIL-1 cells could stimulate in vitro cultured hypothalamic neurocytes to release PGE2, with a good correlation between the inhibition and generation effects of PGE2 and the drug concentration.
authors.affiliation: Beijing Institute for Drug Control, Beijing 100035, China. zepingzuo@126.com
authors.forename: Ze-Ping
authors.initials: ZP
authors.lastname: Zuo
authors.affiliation: Beijing Institute for Drug Control, Beijing 100035, China.
authors.forename: Zhi-Bin
authors.initials: ZB
authors.lastname: Wang
authors.affiliation: Beijing Institute for Drug Control, Beijing 100035, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Beijing Institute for Drug Control, Beijing 100035, China.
authors.forename: Yu-Dong
authors.initials: YD
authors.lastname: Guo
authors.affiliation: Beijing Institute for Drug Control, Beijing 100035, China.
authors.forename: Bi-Song
authors.initials: BS
authors.lastname: Wang
authors.affiliation: Beijing University of Traditional Chinese Medicine, Beijing 100102, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Su
authors.affiliation: Beijing University of Traditional Chinese Medicine, Beijing 100102, China.
authors.forename: Cheng-Cheng
authors.initials: CC
authors.lastname: Song
mesh_terms: D000818:Animals; D001681:Biological Assay; D029972:Bupleurum / Q000737:chemistry*; D002478:Cells, Cultured; D015232:Dinoprostone / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D007031:Hypothalamus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004365:Drugs, Chinese Herbal; D015232:Dinoprostone