title: Quantitative proteomics study of larval settlement in the Barnacle Balanus amphitrite.
abstract: Barnacles are major sessile components of the intertidal areas worldwide, and also one of the most dominant fouling organisms in fouling communities. Larval settlement has a crucial ecological effect not only on the distribution of the barnacle population but also intertidal community structures. However, the molecular mechanisms involved in the transition process from the larval to the juvenile stage remain largely unclear. In this study, we carried out comparative proteomic profiles of stage II nauplii, stage VI nauplii, cyprids, and juveniles of the barnacle Balanus amphitrite using label-free quantitative proteomics, followed by the measurement of the gene expression levels of candidate proteins. More than 700 proteins were identified at each stage; 80 were significantly up-regulated in cyprids and 95 in juveniles vs other stages. Specifically, proteins involved in energy and metabolism, the nervous system and signal transduction were significantly up-regulated in cyprids, whereas proteins involved in cytoskeletal remodeling, transcription and translation, cell proliferation and differentiation, and biomineralization were up-regulated in juveniles, consistent with changes associated with larval metamorphosis and tissue remodeling in juveniles. These findings provided molecular evidence for the morphological, physiological and biological changes that occur during the transition process from the larval to the juvenile stages in B. amphitrite.
authors.affiliation: KAUST Global Collaborative Research Program, Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong SAR, China.
authors.forename: Zhang-Fan
authors.initials: ZF
authors.lastname: Chen
authors.affiliation: Bioscience Core Laboratory, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia.
authors.forename: Huoming
authors.initials: H
authors.lastname: Zhang
authors.affiliation: KAUST Global Collaborative Research Program, Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong SAR, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: KAUST Global Collaborative Research Program, Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong SAR, China.
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Matsumura
authors.affiliation: KAUST Global Collaborative Research Program, Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong SAR, China.
authors.forename: Yue Him
authors.initials: YH
authors.lastname: Wong
authors.affiliation: Integrative Systems Biology Lab, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia.
authors.forename: Timothy
authors.initials: T
authors.lastname: Ravasi
authors.affiliation: KAUST Global Collaborative Research Program, Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong SAR, China.
authors.forename: Pei-Yuan
authors.initials: PY
authors.lastname: Qian
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D020869:Gene Expression Profiling; D008018:Life Cycle Stages / Q000235:genetics*; D053858:Metabolic Networks and Pathways / Q000235:genetics; D058977:Molecular Sequence Annotation; D010802:Phylogeny; D040901:Proteomics / Q000706:statistics & numerical data*; D001468:Thoracica / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D014819:Vitellogenins / Q000145:classification / Q000235:genetics
chemical_list: D060829:Arthropod Proteins; D014819:Vitellogenins