title: Dual-mode hydrodynamic railing and arraying of microparticles for multi-stage signal detection in continuous flow biochemical microprocessors.
abstract: Continuous flow particulate-based microfluidic processors are in critical demand for emerging applications in chemistry and biology, such as point-of-care molecular diagnostics. Challenges remain, however, for accomplishing biochemical assays in which microparticle immobilization is desired or required during intermediate stages of fluidic reaction processes. Here we present a dual-mode microfluidic reactor that functions autonomously under continuous flow conditions to: (i) execute multi-stage particulate-based fluidic mixing routines, and (ii) array select numbers of microparticles during each reaction stage (e.g., for optical detection). We employ this methodology to detect the inflammatory cytokine, interferon-gamma (IFN-γ), via a six-stage aptamer-based sandwich assay.
authors.affiliation: Department of Mechanical Engineering, University of California, Berkeley, USA. rsochol@gmail.com.
authors.forename: Ryan D
authors.initials: RD
authors.lastname: Sochol
authors.forename: Daniel
authors.initials: D
authors.lastname: Corbett
authors.forename: Sarah
authors.initials: S
authors.lastname: Hesse
authors.forename: William E R
authors.initials: WE
authors.lastname: Krieger
authors.forename: Ki Tae
authors.initials: KT
authors.lastname: Wolf
authors.forename: Minkyu
authors.initials: M
authors.lastname: Kim
authors.forename: Kosuke
authors.initials: K
authors.lastname: Iwai
authors.forename: Song
authors.initials: S
authors.lastname: Li
authors.forename: Luke P
authors.initials: LP
authors.lastname: Lee
authors.forename: Liwei
authors.initials: L
authors.lastname: Lin
mesh_terms: D052157:Aptamers, Nucleotide / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D057446:Hydrodynamics; D007371:Interferon-gamma / Q000032:analysis / Q000378:metabolism; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D008863:Microspheres
chemical_list: D052157:Aptamers, Nucleotide; D007371:Interferon-gamma