title: Iso-suillin from the mushroom Suillus flavus induces cell cycle arrest and apoptosis in K562 cell line.
abstract: Iso-suillin, an isomer of suillin that belongs to the prenylphenol class of fungal derivatives, was isolated from petroleum ether extracts of Suillus flavus. The IC50 value of iso-suillin in K562 cells was 0.87 μM, which was lower than the positive control cisplatin (19.33 μM). Iso-suillin-treated K562 cells exhibited an increased rate of apoptosis, mitochondrial membrane potential (MMP) depolarization, and G0/G1 arrest. Western blot analysis revealed that these cells displayed significantly upregulated expression of several apoptosis-related proteins, including cytochrome c, caspase 9, FADD (Fas-associating protein with a novel death domain), caspase 8, caspase 3, and Bax. Moreover, the expression of two anti-apoptosis proteins, NF-κB and Bcl-2, was downregulated. Inhibitors of caspase 9 and caspase 8 protected the K562 cells from apoptosis. Taken together, our results suggest that iso-suillin induces K562 apoptosis through the mitochondrial and death receptor pathways and that iso-suillin may represent a candidate anti-leukemia treatment.
authors.affiliation: College of Life Sciences, Hebei Normal University, No. 20, Road East of 2nd Ring South, Yuhua District, Shijiazhuang 050024, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Hebei Normal University, No. 20, Road East of 2nd Ring South, Yuhua District, Shijiazhuang 050024, China.
authors.forename: Qingshuang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: The Basic Medical College, Hebei Medical University, No. 361, Zhongshan East Road, Shijiazhuang 050017, China.
authors.forename: Junxia
authors.initials: J
authors.lastname: Zhao
authors.affiliation: College of Life Sciences, Hebei Normal University, No. 20, Road East of 2nd Ring South, Yuhua District, Shijiazhuang 050024, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Zhao
authors.affiliation: College of Life Sciences, Hebei Normal University, No. 20, Road East of 2nd Ring South, Yuhua District, Shijiazhuang 050024, China. Electronic address: xiudou882003@163.com.
authors.forename: Jinxiu
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Hebei Normal University, No. 20, Road East of 2nd Ring South, Yuhua District, Shijiazhuang 050024, China. Electronic address: wlian1965@126.com.
authors.forename: Li-an
authors.initials: LA
authors.lastname: Wang
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects*; D004224:Diterpenes; D006801:Humans; D020014:K562 Cells; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D010636:Phenols
chemical_list: D004224:Diterpenes; D010636:Phenols; C061636:suillin
keywords: Apoptosis; Bax/Bcl-2; Caspase family; Cytochrome c; G0/G1 arrest; Mitochondrial membrane potential