title: Effect of metformin on the fertilizing ability of mouse spermatozoa.
abstract: Numerous antioxidants have been added to cryopreservation media with varied success. The biguanide, metformin, commonly used for the treatment of type II diabetes, possesses properties impacting metabolism control that have not been yet assessed in cryopreservation protocols. The aim of this experiment was to; (i) determine the effect of metformin on fresh spermatozoa properties; and (ii) to assess positive or negative effects of metformin in post-thaw function and fertilizing capacity of mouse spermatozoa when used in cryopreservation media. The experiments have shown that the presence of metformin in fresh semen did not induce negative effects on spermatozoa quality, except a slight reduction in sperm motility at 5000μM metformin. However, when metformin was included in a cryopreservation protocol, an improvement in the fertilization rate and a reduction in the percentage of abnormal zygotes after in vitro fertilization was observed. In conclusion, metformin did not affect sperm quality at low concentrations (50μM), but its presence in the cryopreservation media could represent a benefit to improve the quality of frozen semen.
authors.affiliation: Unité de Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique, UMR85, F-37380 Nouzilly, France.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Bertoldo
authors.affiliation: Unité de Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique, UMR85, F-37380 Nouzilly, France.
authors.forename: Edith
authors.initials: E
authors.lastname: Guibert
authors.affiliation: Unité de Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique, UMR85, F-37380 Nouzilly, France.
authors.forename: Pauline
authors.initials: P
authors.lastname: Tartarin
authors.affiliation: INRA Centre Val de Loire, Pôle Santé Animale de Tours, Plate-Forme d'Infectiologie Expérimentale, F-37380 Nouzilly, France.
authors.forename: Vanaique
authors.initials: V
authors.lastname: Guillory
authors.affiliation: Unité de Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique, UMR85, F-37380 Nouzilly, France. Electronic address: pascal.froment@tours.inra.fr.
authors.forename: Pascal
authors.initials: P
authors.lastname: Froment
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D005260:Female; D005307:Fertilization in Vitro / Q000187:drug effects*; D008297:Male; D008687:Metformin / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D012662:Semen Preservation / Q000379:methods*; D013081:Sperm Motility / Q000187:drug effects
chemical_list: D000975:Antioxidants; D003451:Cryoprotective Agents; D008687:Metformin
keywords: Anti-oxidant; Cryopreservation; IVF; Metformin; Motility; Mouse sperm