title: Adalimumab ameliorates abdominal aorta cross clamping which induced liver injury in rats.
abstract: The aim of this study was to investigate the possible protective effects of adalimumab (ADA) on cell damage in rat liver tissue during ischemia/reperfusion (I/R) injury of infrarenal abdominal aorta. Thirty male Wistar-albino rats were divided into three groups: control, I/R, and I/R+ADA, each group containing 10 animals. Laparotomy without I/R injury was performed in the control group animals. Laparotomy in the I/R group was followed by two hours of infrarenal abdominal aortic cross ligation and then two hours of reperfusion. ADA (50 mg/kg) was administered intraperitoneally as a single dose, to the I/R+ADA group, five days before I/R. The tumor necrosis factor-alpha (TNF-α) (pg/mg protein) and nitric oxide (NO) (µmol/g protein) levels in the I/R group (430.8 ± 70.1, 8.0 ± 1.1, resp.) were significantly higher than those in the I/R+ADA group (338.0 ± 71.6, P = 0.006; 6.3 ± 1.2, P = 0.008) and the control group (345.5 ± 53.3, P = 0.008; 6.5 ± 1.5, P = 0.010, resp.). I/R causes severe histopathological injury to the liver tissue, but ADA leads to much less histopathological changes. ADA treatment significantly decreased the severity of liver I/R injury. ADA pretreatment may have protective effects on experimental liver injury.
authors.affiliation: Department of Internal Medicine, School of Medicine, Recep Tayyip Erdogan University, 53100 Rize, Turkey.
authors.forename: Erkan
authors.initials: E
authors.lastname: Cure
authors.affiliation: Department of Biochemistry, School of Medicine, Recep Tayyip Erdogan University, 53100 Rize, Turkey.
authors.forename: Medine
authors.initials: M
authors.lastname: Cumhur Cure
authors.affiliation: Department of Histology and Embryology, School of Medicine, Recep Tayyip Erdogan University, 53100 Rize, Turkey.
authors.forename: Levent
authors.initials: L
authors.lastname: Tumkaya
authors.affiliation: Department of Histology and Embryology, School of Medicine, Recep Tayyip Erdogan University, 53100 Rize, Turkey.
authors.forename: Yildiray
authors.initials: Y
authors.lastname: Kalkan
authors.affiliation: Department of Surgery, School of Medicine, Recep Tayyip Erdogan University, 53100 Rize, Turkey.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Aydin
authors.affiliation: Department of Biochemistry, School of Medicine, Recep Tayyip Erdogan University, 53100 Rize, Turkey.
authors.forename: Aynur
authors.initials: A
authors.lastname: Kirbas
authors.affiliation: Department of Gastroenterology, School of Medicine, Recep Tayyip Erdogan University, 53100 Rize, Turkey.
authors.forename: Arif
authors.initials: A
authors.lastname: Yilmaz
authors.affiliation: Department of Internal Medicine, School of Medicine, Recep Tayyip Erdogan University, 53100 Rize, Turkey.
authors.forename: Suleyman
authors.initials: S
authors.lastname: Yuce
authors.affiliation: Department of Surgery, School of Medicine, Recep Tayyip Erdogan University, 53100 Rize, Turkey.
authors.forename: Ahmet Fikret
authors.initials: AF
authors.lastname: Yücel
mesh_terms: D000068879:Adalimumab; D000818:Animals; D061067:Antibodies, Monoclonal, Humanized / Q000494:pharmacology* / Q000627:therapeutic use; D001012:Aorta, Abdominal / Q000293:injuries*; D007150:Immunohistochemistry; D008099:Liver / Q000737:chemistry / Q000187:drug effects*; D008297:Male; D009569:Nitric Oxide / Q000032:analysis / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy / Q000378:metabolism*; D018709:Statistics, Nonparametric; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000378:metabolism
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D000068879:Adalimumab