title: Knockdown of 14-3-3ζ enhances radiosensitivity and radio-induced apoptosis in CD133(+) liver cancer stem cells.
abstract: 14-3-3ζ is related to many cancer survival cellular processes. In a previous study, we showed that silencing 14-3-3ζ decreases the resistance of hepatocellular carcinoma (HCC) to chemotherapy. In this study, we investigated whether silencing 14-3-3ζ affects the radioresistance of cancer stem-like cells (CSCs) in HCC. Knockdown of 14-3-3ζ decreased cell viability and the number of spheres by reducing radioresistance in CSCs after γ-irradiation (IR). Furthermore, the levels of pro-apoptotic proteins were upregulated in CSCs via silencing 14-3-3ζ after IR. These results suggest that 14-3-3ζ knockdown enhances radio-induced apoptosis by reducing radioresistance in liver CSCs.
authors.affiliation: Liver Research Center and WHO Collaborating Center of Viral hepatitis, Catholic University of Korea, Seoul, Korea.
authors.forename: Young Ki
authors.initials: YK
authors.lastname: Lee
authors.affiliation: Liver Research Center and WHO Collaborating Center of Viral hepatitis, Catholic University of Korea, Seoul, Korea.
authors.forename: Wonhee
authors.initials: W
authors.lastname: Hur
authors.affiliation: Liver Research Center and WHO Collaborating Center of Viral hepatitis, Catholic University of Korea, Seoul, Korea.
authors.forename: Sung Won
authors.initials: SW
authors.lastname: Lee
authors.affiliation: Liver Research Center and WHO Collaborating Center of Viral hepatitis, Catholic University of Korea, Seoul, Korea.
authors.forename: Sung Woo
authors.initials: SW
authors.lastname: Hong
authors.affiliation: Liver Research Center and WHO Collaborating Center of Viral hepatitis, Catholic University of Korea, Seoul, Korea.
authors.forename: Sung Woo
authors.initials: SW
authors.lastname: Kim
authors.affiliation: Liver Research Center and WHO Collaborating Center of Viral hepatitis, Catholic University of Korea, Seoul, Korea.
authors.forename: Jung Eun
authors.initials: JE
authors.lastname: Choi
authors.affiliation: Liver Research Center and WHO Collaborating Center of Viral hepatitis, Catholic University of Korea, Seoul, Korea.
authors.forename: Seung Kew
authors.initials: SK
authors.lastname: Yoon
mesh_terms: D048948:14-3-3 Proteins / Q000235:genetics / Q000378:metabolism*; D000071916:AC133 Antigen; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D005720:Gamma Rays; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000528:radiation effects*; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D011836:Radiation Tolerance
chemical_list: D048948:14-3-3 Proteins; D000071916:AC133 Antigen; D015703:Antigens, CD; D051017:Apoptosis Regulatory Proteins; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides