title: CD2 and CD8α define porcine γδ T cells with distinct cytokine production profiles.
abstract: γδ T cells are a remarkably prominent T-cell subset in swine with a high prevalence in blood. Phenotypic analyses in this study showed that CD2(-) γδ T cells in their vast majority had a CD8α(-)SLA-DR(-)CD27(+) phenotype. CD2(+) γδ T cells dominated in spleen and lymph nodes and had a more heterogeneous phenotype. CD8α(+)SLA-DR(-)CD27(+) γδ T cells prevailed in blood, spleen and lymph nodes whereas in liver a CD8α(+)SLA-DR(+)CD27(-) phenotype dominated, indicating an enrichment of terminally differentiated γδ T cells. γδ T cells were also investigated for their potential to produce IFN-γ, TNF-α and IL-17A. Within CD2(+) γδ T cells, IFN-γ and TNF-α single-producers as well as IFN-γ/TNF-α double-producers dominated, which had a CD8α(+)CD27(+/-) phenotype. IL-17A-producing γδ T cells were only found within CD2(-) γδ T cells, mostly co-produced TNF-α and had a rare CD8α(+)CD27(-) phenotype. However, quantitatively TNF-α single-producers strongly dominated within CD2(-) γδ T cells. In summary, our data identify CD2 and CD8α as important molecules correlating with functional differentiation.
authors.affiliation: Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Corinna
authors.initials: C
authors.lastname: Sedlak
authors.affiliation: Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Martina
authors.initials: M
authors.lastname: Patzl
authors.affiliation: Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Armin
authors.initials: A
authors.lastname: Saalmüller
authors.affiliation: Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria. Electronic address: wilhelm.gerner@vetmeduni.ac.at.
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Gerner
mesh_terms: D000818:Animals; D018801:CD2 Antigens / Q000378:metabolism*; D016827:CD8 Antigens / Q000378:metabolism*; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D008099:Liver / Q000166:cytology; D008198:Lymph Nodes / Q000166:cytology; D009928:Organ Specificity; D013154:Spleen / Q000166:cytology; D034421:Sus scrofa / Q000033:anatomy & histology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*; D013950:Thymus Gland / Q000166:cytology
chemical_list: D018801:CD2 Antigens; D016827:CD8 Antigens; C409469:CD8 antigen, alpha chain; D016207:Cytokines
keywords: IFN-γ; IL-17A; Phenotype; Swine; TNF-α; γδ T cells