title: The subtype CD200-positive, chorionic mesenchymal stem cells from the placenta promote regeneration of human hepatocytes.
abstract: Human placental mesenchymal stem cells (hPMSCs), for the treatment of fulminant hepatic failure, have been widely studied. Only a few studies have investigated the effect of the subtype CD200(+)hPMSCs on regeneration of human hepatocytes. CD200(+)hPMSCs can down-regulate activity of several immunocytes and suppress TNF-α secretion from macrophages via the CD200-CD200R axis. We have investigated the influence of CD200-positive human placenta chorionic mesenchymal stem cells (CD200(+)hPCMSCs) on metabolism, proliferation and apoptosis of human hepatocytes in vitro. CD200(+)hPCMSCs promote urea synthesis, albumin secretion and hepatocytes proliferation at co-culture ratios of 1:1 and 3:1. Additionally, CD200(+)hPCMSCs inhibit hepatocyte apoptosis via up-regulation of an anti-apoptotic protein, Bcl-xL. Thus, CD200(+)hPCMSCs can provide supportive benefit for the regeneration of human hepatocytes and also have immunosuppressive properties. Therefore, CD200(+)hPCMSCs may be an ideal candidate for stem cell-based therapy in hepatic failure.
authors.affiliation: Department of Hepatobiliary Surgery, The Third Central Clinical College of Tianjin Medical University, Tianjin, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Zhengyan
authors.initials: Z
authors.lastname: Zhu
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Luo
authors.forename: Yingtang
authors.initials: Y
authors.lastname: Gao
authors.forename: Zhi
authors.initials: Z
authors.lastname: Du
mesh_terms: D015703:Antigens, CD / Q000032:analysis*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D022781:Hepatocytes / Q000502:physiology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D010920:Placenta / Q000166:cytology; D011247:Pregnancy
chemical_list: D015703:Antigens, CD; C042739:antigens, CD200