title: High bioethanol titre from Manihot glaziovii through fed-batch simultaneous saccharification and fermentation in Automatic Gas Potential Test System.
abstract: A process for the production of high bioethanol titre was established through fed-batch and simultaneous saccharification and fermentation (FB-SSF) of wild, non-edible cassava Manihot glaziovii. FB-SSF allowed fermentation of up to 390g/L of starch-derived glucose achieving high bioethanol concentration of up to 190g/L (24% v/v) with yields of around 94% of the theoretical value. The wild cassava M. glaziovii starch is hydrolysable with a low dosage of amylolytic enzymes (0.1-0.15% v/w, Termamyl® and AMG®). The Automatic Gas Potential Test System (AMPTS) was adapted to yeast ethanol fermentation and demonstrated to be an accurate, reliable and flexible device for studying the kinetics of yeast in SSF and FB-SSF. The bioethanol derived stoichiometrically from the CO2 registered in the AMPTS software correlated positively with samples analysed by HPLC (R(2)=0.99).
authors.affiliation: Division of Biotechnology, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden; Department of Molecular Biology and Biotechnology, College of Natural and Applied Sciences, Uvumbuzi Road, Mwalimu J.K. Nyerere Mlimani Campus, University of Dar-es Salaam, P.O. Box 35179, Dar-es Salaam, Tanzania. Electronic address: ansemoshi@yahoo.co.uk.
authors.forename: Anselm P
authors.initials: AP
authors.lastname: Moshi
authors.affiliation: Division of Biotechnology, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden. Electronic address: carlacrespo123@gmail.com.
authors.forename: Carla F
authors.initials: CF
authors.lastname: Crespo
authors.affiliation: Division of Biotechnology, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden. Electronic address: malikbadshah@gmail.com.
authors.forename: Malik
authors.initials: M
authors.lastname: Badshah
authors.affiliation: Department of Molecular Biology and Biotechnology, College of Natural and Applied Sciences, Uvumbuzi Road, Mwalimu J.K. Nyerere Mlimani Campus, University of Dar-es Salaam, P.O. Box 35179, Dar-es Salaam, Tanzania. Electronic address: kenhosea2@gmail.com.
authors.forename: Kenneth M M
authors.initials: KM
authors.lastname: Hosea
authors.affiliation: Department of Molecular Biology and Biotechnology, College of Natural and Applied Sciences, Uvumbuzi Road, Mwalimu J.K. Nyerere Mlimani Campus, University of Dar-es Salaam, P.O. Box 35179, Dar-es Salaam, Tanzania. Electronic address: anthony.mshandete@yahoo.com.
authors.forename: Anthony Manoni
authors.initials: AM
authors.lastname: Mshandete
authors.affiliation: Division of Biotechnology, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden. Electronic address: Bo.Mattiasson@biotek.lu.se.
authors.forename: Bo
authors.initials: B
authors.lastname: Mattiasson
mesh_terms: D061249:Batch Cell Culture Techniques / Q000379:methods*; D056804:Biofuels; D050260:Carbohydrate Metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D006868:Hydrolysis; D002365:Manihot / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013997:Time Factors; D000516:alpha-Amylases / Q000378:metabolism
chemical_list: D056804:Biofuels; D002245:Carbon Dioxide; D000431:Ethanol; D000516:alpha-Amylases
keywords: Automatic Gas Potential Test System; Bioethanol; Fed-batch; Manihot glaziovii; Simultaneous saccharification and fermentation