title: New phenotypic characteristics of three tmm alleles in Arabidopsis thaliana.
abstract: Three new tmm mutants were isolated and showed differential phenotypes from tmm - 1 , and TMM overexpression led to abnormal leaf trichomes. TOO MANY MOUTH (TMM) plays a significant role in the stomatal signal transduction pathway, which involves in the regulation of stomatal distribution and patterning. Three mutants with clustered stomata were isolated and identified as new alleles of tmm. tmm-4 mutation included a base transversion from adenine to thymidine in position 1,033 of the TMM coding region and resulted in premature termination of translation at position 345 of TMM. tmm-5 had a base transition from cytosine to thymidine in 244 of TMM and translated 82 amino acids before premature termination. tmm-6 mutation took a base transition from guanine to adenine in 463 of TMM and changed a glycine (Gly) to an arginine (Arg) in position 155 of the protein. tmm-6 had an evident reduction of stomatal clusters and fewer stomata in cluster compared with other tmm alleles, possibly due to decreased level of entry divisions in cells next to two stomata or their precursors. tmm-5 and tmm-6 were hypersensitive to abscisic acid (ABA) in seedling growth and seed germination, while tmm-4 was defective in response to ABA during seed dormancy, suggesting that TMM was involved in ABA signaling transduction. Interestingly, overexpression of TMM resulted in the reduction of leaf trichomes and their branches, and this might reveal a new function of TMM in trichome development.
authors.affiliation: Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, School of Life Sciences, Lanzhou University, 730000, Gansu, China.
authors.forename: Longfeng
authors.initials: L
authors.lastname: Yan
authors.forename: Xi
authors.initials: X
authors.lastname: Cheng
authors.forename: Ruiling
authors.initials: R
authors.lastname: Jia
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Qin
authors.forename: Liping
authors.initials: L
authors.lastname: Guan
authors.forename: Hang
authors.initials: H
authors.lastname: Du
authors.forename: Suiwen
authors.initials: S
authors.lastname: Hou
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D000483:Alleles; D017360:Arabidopsis / Q000033:anatomy & histology / Q000235:genetics* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D035264:Flowers / Q000033:anatomy & histology / Q000235:genetics / Q000502:physiology; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D018525:Germination; D009154:Mutation; D010641:Phenotype; D010937:Plant Growth Regulators / Q000378:metabolism*; D018515:Plant Leaves / Q000033:anatomy & histology / Q000235:genetics / Q000502:physiology; D018547:Plant Stems / Q000033:anatomy & histology / Q000235:genetics / Q000502:physiology; D054046:Plant Stomata / Q000033:anatomy & histology / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified; D036226:Seedlings / Q000033:anatomy & histology / Q000235:genetics / Q000502:physiology; D015398:Signal Transduction; D064201:Trichomes / Q000033:anatomy & histology / Q000235:genetics / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; D010937:Plant Growth Regulators; C459090:too many mouths protein, Arabidopsis; D000040:Abscisic Acid