title: Chemical composition, antibacterial, antifungal and antioxidant activities of Algerian Eryngium tricuspidatum L. essential oil.
abstract: This study describes the chemical composition and the antibacterial, antifungal and antioxidant activities of the essential oil extracted from aerial parts of the Algerian Eryngium tricuspidatum L., obtained by hydrodistillation and analysed by using the combination of gas chromatography (GC) and GC/mass spectrometry. A total of 63 compounds were identified accounting for 93.1% of the total oil. Chemical composition of oil was characterised by a high proportion of oxygenated sesquiterpenes (49.6%) among which α-bisabolol (32.6%) was the predominant compound. The sesquiterpene hydrocarbons represent the second major fraction (31.9%) with α-curcumene (6.5%) being the predominant one. Antibacterial and antifungal activities of the oil were tested using the micro-well determination of minimum inhibitory concentration (MIC) assay against eleven bacteria and two Candida species. It was found that the aerial parts of E. tricuspidatum exhibited interesting antibacterial and anticandidal activities (MIC = 9 μg/mL against several strains of bacteria and MIC = 4.6 μg/mL against Candida albicans). The antioxidant effect of this oil was evaluated using the 2,2-diphenyl-l-1-picrylhydrazil (DPPH) and ferric reducing antioxidant power (FRAP) assays. Results revealed significant activities (DPPH method: IC₅₀ = 510 μg/mL; FRAP assay: reducing power of oil increases from 0.0188 at 5 μg/mL to 0.5016 at 1000 μg/mL).
authors.affiliation: a Antibiotics, Antifungal: Physical Chemistry, Synthesis and Biological Activity Laboratory, Department of Biology, Faculty of Natural Sciences and Life Sciences and Earth and the Universe , University of Tlemcen , P.O. Box 119, Tlemcen 13000 , Algeria.
authors.forename: Djamila
authors.initials: D
authors.lastname: Merghache
authors.forename: Zahia
authors.initials: Z
authors.lastname: Boucherit-Otmani
authors.forename: Salima
authors.initials: S
authors.lastname: Merghache
authors.forename: Ilyas
authors.initials: I
authors.lastname: Chikhi
authors.forename: Chaouki
authors.initials: C
authors.lastname: Selles
authors.forename: Kebir
authors.initials: K
authors.lastname: Boucherit
mesh_terms: D000462:Algeria; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects; D031049:Eryngium / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D008826:Microbial Sensitivity Tests; D000081222:Monocyclic Sesquiterpenes; D009822:Oils, Volatile / Q000737:chemistry*; D010851:Picrates / Q000494:pharmacology; D010938:Plant Oils / Q000737:chemistry*; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D000935:Antifungal Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D000081222:Monocyclic Sesquiterpenes; D009822:Oils, Volatile; D010851:Picrates; D010938:Plant Oils; D012717:Sesquiterpenes; C004497:bisabolol; C086829:alpha-curcumene; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: DPPH antioxidant power; Eryngium tricuspidatum L.; GC/MS; antibacterial and antifungal activities; essential oil; ferric reducing antioxidant power assay; α-bisabolol