title: In vitro evaluation of human osteoblast-like cell proliferation and attachment on nanostructured fluoridated hydroxyapatite.
abstract: The effect of the fluorine content and nano-structure of fluoridated hydroxyapatite (FHA) on human osteoblast-like (HO) cell behavior were investigated. FHA nanopowders and bulk nanostructured FHA, produced via mechanical alloying and two-step sintering, respectively, were used. The cytotoxicity of FHA nanopowders was assessed by MTT. Cell attachment to the surface of the bulk nanostructured FHA was evaluated by culturing of HO cells. Although HO cells proliferated 10 % more in contact with FHA nanopowders compared to culture medium without FHA nanopowders, an increase in the fluorine content of FHA caused a delay in the cell proliferation by about 2 days. Cell attachment on the bulk nanostructured FHA did not change the fluorine content.
authors.affiliation: Biomaterials Research Group, Department of Materials Engineering, Isfahan University of Technology, 8415683111, Esfahān, Iran, mh.esnaashary@yahoo.com.
authors.forename: Mohammadhossein
authors.initials: M
authors.lastname: Esnaashary
authors.forename: Mohammadhossein
authors.initials: M
authors.lastname: Fathi
authors.forename: Mahdi
authors.initials: M
authors.lastname: Ahmadian
mesh_terms: D002448:Cell Adhesion; D049109:Cell Proliferation; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D006882:Hydroxyapatites / Q000378:metabolism* / Q000633:toxicity; D010006:Osteoblasts / Q000502:physiology*
chemical_list: D006882:Hydroxyapatites; C028869:fluor-hydroxylapatite