title: Detection of glutathione within single mice hepatocytes using microfluidic chips coupled with a laser-induced fluorescence system.
abstract: A rapid and accurate detection of glutathione (GSH) content in single cells is important to the early diagnosis and prevention of diseases. A microfluidic system allows the manipulation of trace amounts of reagents and single cells in a simple and cheap glass chip coupled with laser induced fluorescence (LIF) detection. 2,3-Naphthalenedicarboxaldehyde (NDA) was used as the derivatization reagent to label GSH in cells. Microchannel surface derivatization and optimization of injection and separation were investigated in detail, and then the GSH in single mice hepatocyte was separated and detected under optimum conditions with a linear range of 5×10(-4) M~5×10(-3) M and a detection limit of 4.47×10(-5) M. This study provides a simple and effective method for rapid GSH detection in single cells using few reagents.
authors.affiliation: School of Environmental Science and Engineering, China-America CRC for Environment & Health, Shandong University, 27# Shanda South Road, Shandong Province, Jinan 250100, PR China.
authors.forename: Minglu
authors.initials: M
authors.lastname: Hao
authors.affiliation: School of Environmental Science and Engineering, China-America CRC for Environment & Health, Shandong University, 27# Shanda South Road, Shandong Province, Jinan 250100, PR China. Electronic address: rutaoliu@sdu.edu.cn.
authors.forename: Rutao
authors.initials: R
authors.lastname: Liu
authors.affiliation: School of Environmental Science and Engineering, China-America CRC for Environment & Health, Shandong University, 27# Shanda South Road, Shandong Province, Jinan 250100, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Environmental Science and Engineering, China-America CRC for Environment & Health, Shandong University, 27# Shanda South Road, Shandong Province, Jinan 250100, PR China.
authors.forename: Yating
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Environmental Science and Engineering, China-America CRC for Environment & Health, Shandong University, 27# Shanda South Road, Shandong Province, Jinan 250100, PR China.
authors.forename: Mingyang
authors.initials: M
authors.lastname: Jing
mesh_terms: D000818:Animals; D004560:Electricity; D005453:Fluorescence; D005978:Glutathione / Q000737:chemistry / Q000378:metabolism*; D022781:Hepatocytes / Q000737:chemistry / Q000378:metabolism*; D007834:Lasers; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D009281:Naphthalenes / Q000378:metabolism
chemical_list: D009281:Naphthalenes; C027717:2,3-naphthalenedicarboxaldehyde; D005978:Glutathione
keywords: GSH; Laser induced fluorescence; Mice hepatocytes; Microfluidic system; NDA