title: Transcriptome profile of Trichoderma harzianum IOC-3844 induced by sugarcane bagasse.
abstract: Profiling the transcriptome that underlies biomass degradation by the fungus Trichoderma harzianum allows the identification of gene sequences with potential application in enzymatic hydrolysis processing. In the present study, the transcriptome of T. harzianum IOC-3844 was analyzed using RNA-seq technology. The sequencing generated 14.7 Gbp for downstream analyses. De novo assembly resulted in 32,396 contigs, which were submitted for identification and classified according to their identities. This analysis allowed us to define a principal set of T. harzianum genes that are involved in the degradation of cellulose and hemicellulose and the accessory genes that are involved in the depolymerization of biomass. An additional analysis of expression levels identified a set of carbohydrate-active enzymes that are upregulated under different conditions. The present study provides valuable information for future studies on biomass degradation and contributes to a better understanding of the role of the genes that are involved in this process.
authors.affiliation: Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Maria Augusta Crivelente
authors.initials: MA
authors.lastname: Horta
authors.affiliation: Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Renato
authors.initials: R
authors.lastname: Vicentini
authors.affiliation: Brazilian Bioethanol Science and Technology Laboratory (CTBE), Brazilian Center of Research in Energy and Materials (CNPEM), Campinas, SP, Brazil.
authors.forename: Priscila da Silva
authors.initials: Pda S
authors.lastname: Delabona
authors.affiliation: Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Prianda
authors.initials: P
authors.lastname: Laborda
authors.affiliation: Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Aline
authors.initials: A
authors.lastname: Crucello
authors.affiliation: Brazilian Bioethanol Science and Technology Laboratory (CTBE), Brazilian Center of Research in Energy and Materials (CNPEM), Campinas, SP, Brazil.
authors.forename: Sindélia
authors.initials: S
authors.lastname: Freitas
authors.affiliation: Physics Institute of São Carlos, University of São Paulo (USP), São Carlos, SP, Brazil ; Institute of Science and Technology, Federal University of São Paulo (UNIFESP), São José dos Campos, SP, Brazil.
authors.forename: Reginaldo Massanobu
authors.initials: RM
authors.lastname: Kuroshu
authors.affiliation: Physics Institute of São Carlos, University of São Paulo (USP), São Carlos, SP, Brazil.
authors.forename: Igor
authors.initials: I
authors.lastname: Polikarpov
authors.affiliation: Brazilian Bioethanol Science and Technology Laboratory (CTBE), Brazilian Center of Research in Energy and Materials (CNPEM), Campinas, SP, Brazil.
authors.forename: José Geraldo da Cruz
authors.initials: JG
authors.lastname: Pradella
authors.affiliation: Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, SP, Brazil ; Department of Plant Biology, Biology Institute, University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Anete Pereira
authors.initials: AP
authors.lastname: Souza
mesh_terms: D002480:Cellulase / Q000235:genetics / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D030541:Databases, Genetic; D020869:Gene Expression Profiling; D005800:Genes, Fungal / Q000235:genetics; D058977:Molecular Sequence Annotation; D031786:Saccharum / Q000737:chemistry*; D017423:Sequence Analysis, RNA; D014242:Trichoderma / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*
chemical_list: D002482:Cellulose; C027433:bagasse; D002480:Cellulase