title: Spray-painted human fibronectin coating as an effective strategy to enhance graft ligamentization of a polyethylene terephthalate artificial ligament.
abstract: To enhance graft ligamentization after anterior cruciate ligament (ACL) reconstruction, human fibronectin (FN) was coated on polyethylene terephthalate (PET) ligaments by spray painting. The FN-coated PET ligaments were investigated in vitro using rat mesenchymal stromal cells (MSCs). MSCs cultured on FN-coated grafts resulted in similar cell densities and amounts of proliferating cells with control grafts without coating. The FN-coated group not only gave rise to MSC-derived collagen-like tissues but also enhanced the expression of collagen-I gene. Furthermore, rat ACL reconstruction models were used to evaluate the effect of the FN coating in vivo. The FN coating significantly promoted new ligament tissue regeneration into the graft fibers. In conclusion, sprayed FN coating had a positive effect to enhance graft ligamentization of PET artificial ligament.
authors.affiliation: Department of Sports Medicine, Huashan Hospital, No. 12, Wulumuqi Zhong Road, Shanghai, 200040, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Yunsheng
authors.initials: Y
authors.lastname: Ge
authors.forename: Shiyi
authors.initials: S
authors.lastname: Chen
mesh_terms: D000818:Animals; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology*; D005353:Fibronectins / Q000737:chemistry / Q000494:pharmacology*; D048091:Guided Tissue Regeneration / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D008022:Ligaments / Q000737:chemistry / Q000502:physiology; D008297:Male; D008422:Materials Testing; D011093:Polyethylene Terephthalates / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D011093:Polyethylene Terephthalates