title: [Analysis of N-glycosylated protein and N-glycans in human plasma by hydrophilic enrichment strategy].
abstract: N-glycosylation of proteins is one of the most important post-translational modifications (PTM). Many diagnostic biomarkers and therapeutic targets are glycosylated proteins. However, it is still a big challenge to identify glycoproteins in human plasma, because of the high dynamic range and microheterogeneity of glycosylation. In this work, hydrophilic interaction liquid chromatography (HILIC) enrichment, high-pH reversed-phase prefractionation and high accurate mass spectrometry (MS) analysis were combined to profile the N-glycoproteins in human plasma. In total, 637 N-glycosites from 299 glycoproteins (protein groups) were identified. There were also 31 glycoforms recognized after HILIC enrichment and MS analysis. Among the results, 107 glycosylation sites (16.8%) are newly found. This study provided a simple and reliable strategy for exploring potential N-glycoprotein biomarkers from complicated biological systems.
authors.affiliation: School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Ma
authors.affiliation: School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China.
authors.forename: Yiting
authors.initials: Y
authors.lastname: Pan
authors.affiliation: School of Biological Sciences, Nanyang Technological University, 637551, Singapore.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 102206, China.
authors.forename: Jifeng
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Qian
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 102206, China.
authors.forename: Wantao
authors.initials: W
authors.lastname: Ying
mesh_terms: D002853:Chromatography, Liquid; D006023:Glycoproteins / Q000097:blood* / Q000737:chemistry; D006031:Glycosylation; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D013058:Mass Spectrometry; D010949:Plasma / Q000737:chemistry; D011134:Polysaccharides / Q000097:blood* / Q000737:chemistry; D011499:Protein Processing, Post-Translational
chemical_list: D006023:Glycoproteins; D011134:Polysaccharides