title: Imaging single nanoparticle interactions with human lung cells using fast ion conductance microscopy.
abstract: Experimental data on dynamic interactions between individual nanoparticles and membrane processes at nanoscale, essential for biomedical applications of nanoparticles, remain scarce due to limitations of imaging techniques. We were able to follow single 200 nm carboxyl-modified particles interacting with identified membrane structures at the rate of 15 s/frame using a scanning ion conductance microscope modified for simultaneous high-speed topographical and fluorescence imaging. The imaging approach demonstrated here opens a new window into the complexity of nanoparticle-cell interactions.
authors.affiliation: School of Engineering and Materials Science, Queen Mary University of London , Mile End Rd, London E1 4NS, United Kingdom.
authors.forename: Pavel
authors.initials: P
authors.lastname: Novak
authors.forename: Andrew
authors.initials: A
authors.lastname: Shevchuk
authors.forename: Pakatip
authors.initials: P
authors.lastname: Ruenraroengsak
authors.forename: Michele
authors.initials: M
authors.lastname: Miragoli
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Thorley
authors.forename: David
authors.initials: D
authors.lastname: Klenerman
authors.forename: Max J
authors.initials: MJ
authors.lastname: Lab
authors.forename: Teresa D
authors.initials: TD
authors.lastname: Tetley
authors.forename: Julia
authors.initials: J
authors.lastname: Gorelik
authors.forename: Yuri E
authors.initials: YE
authors.lastname: Korchev
mesh_terms: D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D006801:Humans; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D018715:Microscopy, Video / Q000295:instrumentation / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*