title: Metabonomic study of Wu-tou decoction in adjuvant-induced arthritis rat using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry.
abstract: A urinary metabonomics method based on the ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) had been established to investigate the holistic efficacy of Wu-tou decoction (WTD), a traditional Chinese medicine (TCM) formula used to treat rheumatoid arthritis (RA), in adjuvant-induced arthritis (AIA) rat model. Multivariate statistical approaches, such as principal component analysis (PCA) and orthogonal projection to latent structures squares-discriminant analysis (OPLS-DA) were used to distinguish healthy control group, AIA model group and WTD treated group and find potential biomarkers. There was a clear separation among the three groups in PCA model. Sixteen potential biomarkers had been identified using OPLS-DA, and 11 of them was considered to be in response to therapeutic effects of WTD involved in tryptophan metabolism, phenylalanine metabolism, tricarboxylic acid (TCA) cycle, bile acid biosynthesis, steroid hormone biosynthesis and valine metabolism. In this study, WTD also showed good anti-inflammatory and antioxidant activities in vivo, and it could suppress histopathological changes of AIA rats. There might be a correlation between these results and the regulation of the disturbed metabolites in urine. This study demonstrates that metabonomics is a powerful methodology to gain insight in the mechanism of TCM formula in therapy.
authors.affiliation: Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, Jilin, China; University of the Chinese Academy of Sciences, Beijing 100039, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Qi
authors.affiliation: Collage of Chemistry, Changchun Normal University, Changchun 130032, China.
authors.forename: Shizhe
authors.initials: S
authors.lastname: Li
authors.affiliation: Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, Jilin, China.
authors.forename: Zifeng
authors.initials: Z
authors.lastname: Pi
authors.affiliation: Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, Jilin, China.
authors.forename: Fengrui
authors.initials: F
authors.lastname: Song
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Na
authors.initials: N
authors.lastname: Lin
authors.affiliation: Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, Jilin, China. Electronic address: mslab20@ciac.ac.cn.
authors.forename: Shu
authors.initials: S
authors.lastname: Liu
authors.affiliation: Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, Jilin, China. Electronic address: liuzq@ciac.ac.cn.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D015415:Biomarkers / Q000737:chemistry / Q000378:metabolism / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000378:metabolism* / Q000493:pharmacokinetics / Q000494:pharmacology*; D006614:Hindlimb / Q000187:drug effects / Q000473:pathology; D007596:Joints / Q000187:drug effects / Q000473:pathology; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D055442:Metabolome / Q000187:drug effects*; D055432:Metabolomics; D025341:Principal Component Analysis; D051381:Rats; D017208:Rats, Wistar
chemical_list: D015415:Biomarkers; D004365:Drugs, Chinese Herbal
keywords: Adjuvant-induced arthritis; Mass spectrum; Metabonomics; Traditional Chinese medicine; Wu-tou decoction