title: Genetic diversity and differentiation of Juniperus thurifera in Spain and Morocco as determined by SSR.
abstract: Juniperus thurifera L. is an important tree endemic to the western Mediterranean basin that it is able to grow in semi-arid climates. It nowadays exhibits a disjunct distribution pattern, occurring in North Africa, Spain, France and the Italian Alps. The Strait of Gibraltar has acted as an efficient barrier against gene flow between African and European populations, which are considered different subspecies by some authors. We aimed at describing the intraspecific genetic diversity of J. thurifera in populations from the Iberian Peninsula and Morocco and the phylogeographical relationships among these populations. The ploidy level of J. thurifera was examined and eleven nuclear microsatellites (nSSRs) developed for J. thurifera were assessed for genotyping this species. Six nSSRs were polymorphic and subsequently used to assess the genetic diversity and structure of the studied populations. Genotyping of the tetraploid J. thurifera using nuclear microsatellites supports the separation of Moroccan and Spanish populations into two genetically differentiated groups that correspond to the proposed subspecies africana and thurifera. High values of within population genetic diversity were found, that accounted for 90% of the total genetic variance, while population structure was weak. The estimators of genetic diversity were higher in populations of Spain than in populations of Morocco pointing for a possible loss of genetic diversity during the spread of this species to Africa from Europe.
authors.affiliation: Centro de Ecologia Funcional, Departamento de Ciências da Vida, Universidade de Coimbra, Coimbra, Portugal.
authors.forename: Helena
authors.initials: H
authors.lastname: Teixeira
authors.affiliation: Centro de Ecologia Funcional, Departamento de Ciências da Vida, Universidade de Coimbra, Coimbra, Portugal.
authors.forename: Susana
authors.initials: S
authors.lastname: Rodríguez-Echeverría
authors.affiliation: Centro de Ecologia Funcional, Departamento de Ciências da Vida, Universidade de Coimbra, Coimbra, Portugal.
authors.forename: Cristina
authors.initials: C
authors.lastname: Nabais
mesh_terms: D014644:Genetic Variation; D018653:Juniperus / Q000235:genetics*; D018895:Microsatellite Repeats; D009018:Morocco; D058974:Phylogeography; D011003:Ploidies; D013030:Spain