title: Laccase biosensor based on electrospun copper/carbon composite nanofibers for catechol detection.
abstract: The study compared the biosensing properties of laccase biosensors based on carbon nanofibers (CNFs) and copper/carbon composite nanofibers (Cu/CNFs). The two kinds of nanofibers were prepared by electrospinning and carbonization under the same conditions. Scanning electron microscopy (SEM), X-ray diffraction (XRD) and Raman spectroscopy were employed to investigate the morphologies and structures of CNFs and Cu/CNFs. The amperometric results indicated that the Cu/CNFs/laccase(Lac)/Nafion/glass carbon electrode (GCE) possessed reliable analytical performance for the detection of catechol. The sensitivity of the Cu/CNFs/Lac/Nafion/GCE reached 33.1 μA/mM, larger than that of CNFs/Lac/Nafion/GCE. Meanwhile, Cu/CNFs/Lac/Nafion/GCE had a wider linear range from 9.95 × 10(-6) to 9.76 × 10(-3) M and a lower detection limit of 1.18 μM than CNFs/Lac/Nafion/GCE. Moreover, it exhibited a good repeatability, reproducibility, selectivity and long-term stability, revealing that electrospun Cu/CNFs have great potential in biosensing.
authors.affiliation: Key Laboratory of Eco-textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China. firgexiao@sina.cn.
authors.forename: Jiapeng
authors.initials: J
authors.lastname: Fu
authors.affiliation: Key Laboratory of Eco-textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China. huiqiaoz@163.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Qiao
authors.affiliation: Key Laboratory of Eco-textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China. ldw19900323@163.com.
authors.forename: Dawei
authors.initials: D
authors.lastname: Li
authors.affiliation: Key Laboratory of Eco-textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China. luolei891123@126.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Luo
authors.affiliation: Key Laboratory of Eco-textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China. chenke2327@163.com.
authors.forename: Ke
authors.initials: K
authors.lastname: Chen
authors.affiliation: Key Laboratory of Eco-textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China. qfwei@jiangnan.edu.cn.
authors.forename: Qufu
authors.initials: Q
authors.lastname: Wei
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D002396:Catechols / Q000032:analysis*; D003300:Copper / Q000737:chemistry; D055664:Electrochemical Techniques; D004566:Electrodes; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000737:chemistry* / Q000378:metabolism; D057139:Nanofibers / Q000737:chemistry*
chemical_list: D002396:Catechols; D002244:Carbon; D003300:Copper; D042845:Laccase; C034221:catechol