title: ALDR enhanced endothelial injury in hyperuricemia screened using SILAC.
abstract: BACKGROUND
  The exact etiology of hyperuricemia-induced endothelial injury remains ill-defined. To elucidate the mechanism that leads to endothelial injury in hyperuricemia, we investigated proteins expressed in human umbilical vein endothelial cells (HUVECs) cultured with high concentrations of uric acid (HUA) in vitro.
  
  
  METHODS
  We used stable isotope labeling with amino acids in cell culture (SILAC) combined with LC-MS/MS analysis to compare proteins expressed in HUVECs cultured in media with or without HUA. The results were confirmed by Western blotting. Reactive oxygen species (ROS) were detected using a confocal microscope.
  
  
  RESULTS
  Thirty-nine proteins with various cellular functions were differentially expressed. Among them, aldose reductase (ALDR) protein expression was enhanced significantly, indicating increased aldehyde reductase and oxidoreductase activities. ROS levels decreased when ALDR protein activity was inhibited by siALDR.
  
  
  CONCLUSIONS
  ALDR protein may play an important role in endothelial injury induced by hyperuricemia, and activity of the ALDR protein is associated with oxidative stress.
authors.affiliation: State Key Laboratory of Kidney Disease (2011DAV00088), Kidney Department & Institute of Nephrology, Division of Clinical Internal Medicine, Chinese People's Liberation Army General Hospital, Beijing, P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Quan
authors.initials: Q
authors.lastname: Hong
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Huang
authors.forename: Peng
authors.initials: P
authors.lastname: Xue
authors.forename: Yang
authors.initials: Y
authors.lastname: Lv
authors.forename: Bo
authors.initials: B
authors.lastname: Fu
authors.forename: Xiangmei
authors.initials: X
authors.lastname: Chen
authors.forename: Di
authors.initials: D
authors.lastname: Wu
mesh_terms: D000449:Aldehyde Reductase / Q000096:biosynthesis*; D000975:Antioxidants / Q000009:adverse effects / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic; D061307:Human Umbilical Vein Endothelial Cells / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D033461:Hyperuricemia / Q000201:enzymology* / Q000473:pathology; D007553:Isotope Labeling; D018384:Oxidative Stress; D014527:Uric Acid / Q000009:adverse effects / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D014527:Uric Acid; D000449:Aldehyde Reductase