title: Sudestada1, a Drosophila ribosomal prolyl-hydroxylase required for mRNA translation, cell homeostasis, and organ growth.
abstract: Genome sequences predict the presence of many 2-oxoglutarate (2OG)-dependent oxygenases of unknown biochemical and biological functions in Drosophila. Ribosomal protein hydroxylation is emerging as an important 2OG oxygenase catalyzed pathway, but its biological functions are unclear. We report investigations on the function of Sudestada1 (Sud1), a Drosophila ribosomal oxygenase. As with its human and yeast homologs, OGFOD1 and Tpa1p, respectively, we identified Sud1 to catalyze prolyl-hydroxylation of the small ribosomal subunit protein RPS23. Like OGFOD1, Sud1 catalyzes a single prolyl-hydroxylation of RPS23 in contrast to yeast Tpa1p, where Pro-64 dihydroxylation is observed. RNAi-mediated Sud1 knockdown hinders normal growth in different Drosophila tissues. Growth impairment originates from both reduction of cell size and diminution of the number of cells and correlates with impaired translation efficiency and activation of the unfolded protein response in the endoplasmic reticulum. This is accompanied by phosphorylation of eIF2α and concomitant formation of stress granules, as well as promotion of autophagy and apoptosis. These observations, together with those on enzyme homologs described in the companion articles, reveal conserved biochemical and biological roles for a widely distributed ribosomal oxygenase.
authors.affiliation: Fundación Instituto Leloir, C1405BWE Buenos Aires, Argentina.
authors.forename: Maximiliano J
authors.initials: MJ
authors.lastname: Katz
authors.forename: Julieta M
authors.initials: JM
authors.lastname: Acevedo
authors.forename: Christoph
authors.initials: C
authors.lastname: Loenarz
authors.forename: Diego
authors.initials: D
authors.lastname: Galagovsky
authors.forename: Phebee
authors.initials: P
authors.lastname: Liu-Yi
authors.forename: Marcelo
authors.initials: M
authors.lastname: Pérez-Pepe
authors.forename: Armin
authors.initials: A
authors.lastname: Thalhammer
authors.forename: Rok
authors.initials: R
authors.lastname: Sekirnik
authors.forename: Wei
authors.initials: W
authors.lastname: Ge
authors.forename: Mariana
authors.initials: M
authors.lastname: Melani
authors.forename: María G
authors.initials: MG
authors.lastname: Thomas
authors.forename: Sergio
authors.initials: S
authors.lastname: Simonetta
authors.forename: Graciela L
authors.initials: GL
authors.lastname: Boccaccio
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Schofield
authors.forename: Matthew E
authors.initials: ME
authors.lastname: Cockman
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Ratcliffe
authors.forename: Pablo
authors.initials: P
authors.lastname: Wappner
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D017209:Apoptosis / Q000235:genetics; D001343:Autophagy / Q000235:genetics; D015153:Blotting, Western; D001837:Body Weights and Measures; D002853:Chromatography, Liquid; D017931:DNA Primers / Q000235:genetics; D004330:Drosophila / Q000201:enzymology*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D005216:Fat Body / Q000166:cytology; D005260:Female; D055785:Gene Knockdown Techniques; D006706:Homeostasis / Q000502:physiology*; D006900:Hydroxylation; D064828:Prolyl Hydroxylases / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000502:physiology*; D011499:Protein Processing, Post-Translational / Q000502:physiology; D034622:RNA Interference; D060888:Real-Time Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D056811:Unfolded Protein Response / Q000235:genetics
chemical_list: D017931:DNA Primers; D029721:Drosophila Proteins; D012269:Ribosomal Proteins; D064828:Prolyl Hydroxylases; C000588603:sud1 protein, Drosophila
keywords: dioxygenase; fruit fly; proline; ribosome; tranlational stress