title: Association study of circulating endothelial progenitor cells and plasma prostacyclin levels in pulmonary hypertension rats.
abstract: The aim of this study was to investigate the correlation between circulating endothelial progenitor cell (EPC) levels and plasma prostacyclin (PGI2) concentrations in pulmonary hypertension (PH) rat models. Monocrotaline was used to induce PH, and the number of CD34/Flk-1-double-positive mononuclear cells in peripheral CD45-negative blood was counted by flow cytometry, which reflected EPC circulation. The Greiss method was used to detect the plasma PGI2 concentration. Simple linear regression analysis was used to analyze the relationship between EPC and PGI2. Furthermore, the expressions of cycled EPC, CD34, and Flk-1 were detected under in vitro culture conditions. The mean circulating EPC level in the PH model group (0.013 ± 0.004%) was significantly lower (P < 0.01) than that in the control group (0.036 ± 0.009%). The concentration of plasma PGI2 also significantly decreased (24.36 ± 2.08 vs 58.34 ± 3.31 μM; P < 0.01). There was a positive correlation between EPC and PGI2 (r = 0.870, P < 0.05). Under in vitro culture conditions, the EPC number and the CD34 and Flk-1 expression levels in the PH model group were significantly lower than those in the control group. Together, these results suggest that the occurrence of PH may be associated with decreased plasma PGI2 concentrations, resulting in the reduction of circulating EPC.
authors.affiliation: Department of Respiratory Medicine, Daping Hospital of the Third Military Medical University, Chongqing, China yi_song123@126.com.
authors.forename: Y
authors.initials: Y
authors.lastname: Song
mesh_terms: D053687:Adult Stem Cells / Q000378:metabolism*; D000818:Animals; D018952:Antigens, CD34 / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism*; D011464:Epoprostenol / Q000097:blood*; D006976:Hypertension, Pulmonary / Q000097:blood* / Q000378:metabolism / Q000473:pathology; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000235:genetics / Q000378:metabolism
chemical_list: D018952:Antigens, CD34; D011464:Epoprostenol; D040301:Vascular Endothelial Growth Factor Receptor-2