title: Rutin from Dendropanax morbifera Leveille protects human dopaminergic cells against rotenone induced cell injury through inhibiting JNK and p38 MAPK signaling.
abstract: Dendropanax morbifera Leveille (Araliaceae) is well known in Korean traditional medicine for a variety of diseases. Rotenone is a commonly used neurotoxin to produce in vivo and in vitro Parkinson's disease models. This study was designed to elucidate the processes underlying neuroprotection of rutin, a bioflavonoid isolated from D. morbifera Leveille in cellular models of rotenone-induced toxicity. We found that rutin significantly decreased rotenone-induced generation of reactive oxygen species levels in SH-SY5Y cells. Rutin protected the increased level of intracellular Ca(2+) and depleted level of mitochondrial membrane potential (ΔΨm) induced by rotenone. Furthermore, it prevented the decreased ratio of Bax/Bcl-2 caused by rotenone treatment. Additionally, rutin protected SH-SY5Y cells from rotenone-induced caspase-9 and caspase-3 activation and apoptotic cell death. We also observed that rutin repressed rotenone-induced c-Jun N-terminal kinase and p38 mitogen-activated protein kinase phosphorylation. These results suggest that rutin may have therapeutic potential for the treatment of neurodegenerative diseases associated with oxidative stress.
authors.affiliation: Department of Alternative Medicine, Gwangju University, Gwangju, 503-703, Republic of Korea.
authors.forename: Se-Eun
authors.initials: SE
authors.lastname: Park
authors.forename: Kumar
authors.initials: K
authors.lastname: Sapkota
authors.forename: Jun-Hui
authors.initials: JH
authors.lastname: Choi
authors.forename: Myung-Kon
authors.initials: MK
authors.lastname: Kim
authors.forename: Young Hoi
authors.initials: YH
authors.lastname: Kim
authors.forename: Ki Man
authors.initials: KM
authors.lastname: Kim
authors.forename: Kyung Je
authors.initials: KJ
authors.lastname: Kim
authors.forename: Ha-Na
authors.initials: HN
authors.lastname: Oh
authors.forename: Sung-Jun
authors.initials: SJ
authors.lastname: Kim
authors.forename: Seung
authors.initials: S
authors.lastname: Kim
mesh_terms: D027481:Araliaceae; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D059290:Dopaminergic Neurons / Q000187:drug effects / Q000201:enzymology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D047428:Protein Kinase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D012402:Rotenone / Q000037:antagonists & inhibitors / Q000633:toxicity*; D012431:Rutin / Q000302:isolation & purification / Q000494:pharmacology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D018696:Neuroprotective Agents; D010936:Plant Extracts; D047428:Protein Kinase Inhibitors; D012402:Rotenone; D012431:Rutin; D048031:JNK Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases