title: Method for the analysis of total fluoride in fluoride-releasing dental varnishes.
abstract: Today's fluoride-releasing varnishes (F-varnish) contain a wide variety of ingredients which present analytical challenges for measuring their total fluoride content. This study reports improved methods to measure fluoride content in F-varnishes. Six different commercially available F-varnishes that contain difluorosilane (0.1% F) or NaF (2.26% F) alone or in combination with calcium-phosphates were analyzed. In a vial, 1-3 drops (0.05-0.15 g) of varnish product was dispensed, dissolved in chloroform, equilibrated in TISAB and analyzed via fluoride ion-selective electrode. The average weight percentage of fluoride for all F-varnishes containing NaF ranged from 2.03 to 2.24% F, which is within 90% of the declared label concentration of 2.26% F. Analysis of the difluorosilane-containing product required an additional hydrolysis step. ANOVA found no significant difference between the 5% NaF varnishes at p < 0.05. This method for fluoride analysis yields reliable and reproducible results and can be used for a wide variety of F-varnishes. The standard uncertainty for this method is ±4%. This method may become the basis for national and international standards that ensure the F-varnish products used in clinical practice have the fluoride content declared in the product literature.
authors.forename: C M
authors.initials: CM
authors.lastname: Carey
authors.forename: S S
authors.initials: SS
authors.lastname: Coleman
mesh_terms: D002130:Calcium Phosphates / Q000032:analysis; D002327:Cariostatic Agents / Q000032:analysis* / Q000737:chemistry; D002364:Caseins / Q000737:chemistry; D002725:Chloroform / Q000737:chemistry; D005459:Fluorides / Q000032:analysis* / Q000737:chemistry; D005460:Fluorides, Topical / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D006868:Hydrolysis; D006878:Hydroxides / Q000737:chemistry; D017736:Ion-Selective Electrodes; D017680:Potassium Compounds / Q000737:chemistry; D012821:Silanes / Q000032:analysis; D012969:Sodium Fluoride / Q000032:analysis; D012995:Solubility; D012997:Solvents / Q000737:chemistry
chemical_list: D002130:Calcium Phosphates; D002327:Cariostatic Agents; D002364:Caseins; D005460:Fluorides, Topical; D006878:Hydroxides; D017680:Potassium Compounds; D012821:Silanes; D012997:Solvents; C447032:casein phosphopeptide-amorphous calcium phosphate nanocomplex; D002725:Chloroform; D012969:Sodium Fluoride; D005459:Fluorides; C029943:potassium hydroxide