title: Implementation of laser induced fluorescence in a pulse radiolysis experiment--a new way to analyze resazurin-like reduction mechanisms.
abstract: Resazurin (RNO) reduction by hydrated electrons produces a fluorescent molecule: resorufin (RN). To take advantage of RN fluorescence, a novel setup is designed by implementing fluorescence detection induced by laser in a pulse radiolysis experiment. Time resolved fluorescence spectra were recorded with a fast gated intensified CCD camera during the reduction of RNO from μs to ms. Two 532 nm laser types have been used to describe the short μs range by a 5 ns Q-switch laser and the μs-ms range by a CW DPSS laser. By fitting the simulated model to the experimental data a second order rate constant of 10(9) M(-1) s(-1) was re-evaluated. This method should be considered in the near future in many in situ and real time measurements for evaluating radical production.
authors.affiliation: CEA Saclay, IRAMIS, LIDyL, PCR, Bât546, PC175, F-91191 Gif-sur-Yvette CEDEX, France. gerard.baldacchino@cea.fr.
authors.forename: A
authors.initials: A
authors.lastname: Balcerzyk
authors.forename: G
authors.initials: G
authors.lastname: Baldacchino
mesh_terms: D004583:Electrons; D005609:Free Radicals / Q000737:chemistry; D007834:Lasers; D010078:Oxazines / Q000032:analysis* / Q000737:chemistry; D010084:Oxidation-Reduction; D011675:Pulse Radiolysis / Q000295:instrumentation / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*; D014966:Xanthenes / Q000032:analysis* / Q000737:chemistry
chemical_list: D005609:Free Radicals; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin; C014180:resorufin