title: Effects of microbial and non-microbial factors of liquid anaerobic digestion effluent as inoculum on solid-state anaerobic digestion of corn stover.
abstract: The microbial activity of the inoculum (liquid anaerobic digestion effluent) was altered by autoclaving part of the effluent to study the effect of feedstock to active effluent ratio (F/Ea, 2.2-6.6) and the feedstock to total effluent ratio (F/Et, 2.2 and 4.4) on reactor performance in solid state anaerobic digestion (SS-AD) of corn stover. When the F/Ea ratio was increased from 2.2 to 6.6, methane yield was not significantly reduced; however, reactors became acidified when the F/Et ratio was increased from 2.2 to 4.4. It was concluded that F/Et had a greater effect on methane yields than F/Ea for the range studied in this paper. As analyzed by denaturing gradient gel electrophoresis using PCR amplified 16S rRNA genes, the microbial community underwent dynamic shifts under acidified conditions over 38days of SS-AD. These shifts reflected the acclimation, both adaptive selection and diversification, of the initial inoculated microbial consortia.
authors.affiliation: Department of Food, Agricultural, and Biological Engineering, The Ohio State University/Ohio Agricultural Research and Development Center, 1680 Madison Ave, Wooster, OH 44691, USA.
authors.forename: Jian
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of Food, Agricultural, and Biological Engineering, The Ohio State University/Ohio Agricultural Research and Development Center, 1680 Madison Ave, Wooster, OH 44691, USA.
authors.forename: Fuqing
authors.initials: F
authors.lastname: Xu
authors.affiliation: Department of Food, Agricultural, and Biological Engineering, The Ohio State University/Ohio Agricultural Research and Development Center, 1680 Madison Ave, Wooster, OH 44691, USA.
authors.forename: Zhongjiang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Animal Science, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Jill A
authors.initials: JA
authors.lastname: Stiverson
authors.affiliation: Department of Animal Science, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Zhongtang
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Department of Food, Agricultural, and Biological Engineering, The Ohio State University/Ohio Agricultural Research and Development Center, 1680 Madison Ave, Wooster, OH 44691, USA. Electronic address: li.851@osu.edu.
authors.forename: Yebo
authors.initials: Y
authors.lastname: Li
mesh_terms: D000693:Anaerobiosis; D001105:Archaea / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000379:methods*; D002482:Cellulose / Q000378:metabolism; D058645:Denaturing Gradient Gel Electrophoresis; D005232:Fatty Acids, Volatile / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008697:Methane / Q000378:metabolism; D025341:Principal Component Analysis; D014865:Waste Disposal, Fluid; D014866:Waste Products; D003313:Zea mays / Q000737:chemistry*
chemical_list: D005232:Fatty Acids, Volatile; D014866:Waste Products; D002482:Cellulose; D008697:Methane
keywords: Anaerobic digestion; Biogas; Dry fermentation; Inoculum; Microbial community