title: Polymeric micelles encapsulating photosensitizer: structure/photodynamic therapy efficiency relation.
abstract: Various polymeric micelles were formed from amphiphilic block copolymers, namely, poly(ethyleneoxide-b-ε-caprolactone), poly(ethyleneoxide-b-d,l-lactide), and poly(ethyleneoxide-b-styrene). The micelles were characterized by static and dynamic light scattering, electron microscopy, and asymmetrical flow field-flow fractionation. They all displayed a similar size close to 20 nm. The influence of the chemical structure of the block copolymers on the stability upon dilution of the polymeric micelles was investigated to assess their relevance as carriers for nanomedicine. In the same manner, the stability upon aging was assessed by FRET experiments under various experimental conditions (alone or in the presence of blood proteins). In all cases, a good stability over 48 h for all systems was encountered, with PDLLA copolymer-based systems being the first to release their load slowly. The cytotoxicity and photocytotoxicity of the carriers were examined with or without their load. Lastly, the photodynamic activity was assessed in the presence of pheophorbide a as photosensitizer on 2D and 3D tumor cell culture models, which revealed activity differences between the 2D and 3D systems.
authors.affiliation: Equipe de Biophysique Cellulaire, IPBS-CNRS UMR 5089 , 205 route de Narbonne, BP 64182, 31077 Toulouse Cedex, France.
authors.forename: Laure
authors.initials: L
authors.lastname: Gibot
authors.forename: Arnaud
authors.initials: A
authors.lastname: Lemelle
authors.forename: Ugo
authors.initials: U
authors.lastname: Till
authors.forename: Béatrice
authors.initials: B
authors.lastname: Moukarzel
authors.forename: Anne-Françoise
authors.initials: AF
authors.lastname: Mingotaud
authors.forename: Véronique
authors.initials: V
authors.lastname: Pimienta
authors.forename: Pascale
authors.initials: P
authors.lastname: Saint-Aguet
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Rols
authors.forename: Mireille
authors.initials: M
authors.lastname: Gaucher
authors.forename: Frédéric
authors.initials: F
authors.lastname: Violleau
authors.forename: Christophe
authors.initials: C
authors.lastname: Chassenieux
authors.forename: Patricia
authors.initials: P
authors.lastname: Vicendo
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods; D002734:Chlorophyll / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D004355:Drug Stability; D031541:Fluorescence Resonance Energy Transfer; D045325:HCT116 Cells / Q000187:drug effects; D006801:Humans; D007783:Lactones / Q000737:chemistry; D008027:Light; D008823:Micelles; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000737:chemistry* / Q000494:pharmacology; D011091:Polyesters / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D012542:Scattering, Radiation; D013329:Structure-Activity Relationship
chemical_list: D004337:Drug Carriers; D007783:Lactones; D008823:Micelles; D017319:Photosensitizing Agents; D011091:Polyesters; D011108:Polymers; C405621:poly(epsilon-caprolactone-co-ethylene oxide); C521904:poly(ethylene glycol)-block-poly(epsilon-caprolactone); D002734:Chlorophyll; D011092:Polyethylene Glycols; C032623:pheophorbide a