title: Colloidal polyaniline dispersions: antibacterial activity, cytotoxicity and neutrophil oxidative burst.
abstract: Polyaniline colloids rank among promising application forms of this conducting polymer. Cytotoxicity, antibacterial activity, and neutrophil oxidative burst tests were performed on cells treated with colloidal polyaniline dispersions. The antibacterial effect of colloidal polyaniline against gram-positive and gram-negative bacteria was most pronounced for Bacillus cereus and Escherichia coli, with a minimum inhibitory concentration of 3,500 μg mL(-1). The data recorded on human keratinocyte (HaCaT) and a mouse embryonic fibroblast (NIH/3T3) cell lines using an MTT assay and flow cytometry indicated a concentration-dependent cytotoxicity of colloid, with the absence of cytotoxic effect at around 150 μg mL(-1). The neutrophil oxidative burst test then showed that colloidal polyaniline, in concentrations <150 μg mL(-1), was not able to stimulate the production of reactive oxygen species in neutrophils and whole human blood. However, it worked efficiently as a scavenger of those already formed.
authors.affiliation: Polymer Centre, Faculty of Technology, Tomas Bata University in Zlin, T.G.M. Sq. 5555, 760 01 Zlin, Czech Republic; Centre of Polymer Systems, Tomas Bata University in Zlin, T.G.M. Sq. 5555, 760 01 Zlin, Czech Republic.
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Kucekova
authors.affiliation: Polymer Centre, Faculty of Technology, Tomas Bata University in Zlin, T.G.M. Sq. 5555, 760 01 Zlin, Czech Republic; Centre of Polymer Systems, Tomas Bata University in Zlin, T.G.M. Sq. 5555, 760 01 Zlin, Czech Republic. Electronic address: humpolicek@ft.utb.cz.
authors.forename: Petr
authors.initials: P
authors.lastname: Humpolicek
authors.affiliation: Centre of Polymer Systems, Tomas Bata University in Zlin, T.G.M. Sq. 5555, 760 01 Zlin, Czech Republic; Department of Fat, Surfactant and Cosmetics Technology, Faculty of Technology, Tomas Bata University in Zlin, T.G.M. Sq. 5555, 760 01 Zlin, Czech Republic.
authors.forename: Vera
authors.initials: V
authors.lastname: Kasparkova
authors.affiliation: Institute of Biophysics, Academy of Sciences of the Czech Republic, 612 65 Brno, Czech Republic.
authors.forename: Tomas
authors.initials: T
authors.lastname: Perecko
authors.affiliation: Centre of Polymer Systems, Tomas Bata University in Zlin, T.G.M. Sq. 5555, 760 01 Zlin, Czech Republic.
authors.forename: Marián
authors.initials: M
authors.lastname: Lehocký
authors.affiliation: Department of Fat, Surfactant and Cosmetics Technology, Faculty of Technology, Tomas Bata University in Zlin, T.G.M. Sq. 5555, 760 01 Zlin, Czech Republic.
authors.forename: Iva
authors.initials: I
authors.lastname: Hauerlandová
authors.affiliation: Centre of Polymer Systems, Tomas Bata University in Zlin, T.G.M. Sq. 5555, 760 01 Zlin, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Sáha
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, 162 06 Prague 6, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Stejskal
mesh_terms: D000814:Aniline Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001409:Bacillus cereus / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003102:Colloids / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D051379:Mice; D008826:Microbial Sensitivity Tests; D041681:NIH 3T3 Cells; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010316:Particle Size; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D013211:Staphylococcus aureus / Q000187:drug effects; D013329:Structure-Activity Relationship; D013499:Surface Properties
chemical_list: D000814:Aniline Compounds; D000900:Anti-Bacterial Agents; D003102:Colloids; D017382:Reactive Oxygen Species; C416807:polyaniline
keywords: Antibacterial activity; Apoptosis; Colloidal polyaniline; Cytotoxicity; Necrosis; Oxidative burst