title: Nucleolar GTPase NOG-1 regulates development, fat storage, and longevity through insulin/IGF signaling in C. elegans.
abstract: NOG1 is a nucleolar GTPase that is critical for 60S ribosome biogenesis. Recently, NOG1 was identified as one of the downstream regulators of target of rapamycin (TOR) in yeast. It is reported that TOR is involved in regulating lifespan and fat storage in Caenorhabditis elegans. Here, we show that the nog1 ortholog (T07A9.9: nog-1) in C. elegans regulates growth, development, lifespan, and fat metabolism. A green fluorescence protein (GFP) promoter assay revealed ubiquitous expression of C. elegans nog-1 from the early embryonic to the adult stage. Furthermore, the GFP-tagged NOG-1 protein is localized to the nucleus, whereas the aberrant NOG-1 protein is concentrated in the nucleolus. Functional studies of NOG-1 in C. elegans further revealed that nog-1 knockdown resulted in smaller broodsize, slower growth, increased life span, and more fat storage. Moreover, nog-1 over-expression resulted in decreased life span. Taken together, our data suggest that nog-1 in C. elegans may be an important player in regulating life span and fat storage via the insulin/IGF pathway.
authors.affiliation: Biomedical Research Center, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.
authors.forename: Young-Il
authors.initials: YI
authors.lastname: Kim
authors.forename: Jaya
authors.initials: J
authors.lastname: Bandyopadhyay
authors.forename: Injeong
authors.initials: I
authors.lastname: Cho
authors.forename: Juyeon
authors.initials: J
authors.lastname: Lee
authors.forename: Dae Ho
authors.initials: DH
authors.lastname: Park
authors.forename: Jeong Hoon
authors.initials: JH
authors.lastname: Cho
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins; D002466:Cell Nucleolus / Q000201:enzymology; D017124:Conserved Sequence; D020558:GTP Phosphohydrolases / Q000502:physiology*; D015870:Gene Expression; D018869:Heat-Shock Response; D007328:Insulin / Q000502:physiology*; D050356:Lipid Metabolism; D008136:Longevity; D008969:Molecular Sequence Data; D021381:Protein Transport; D012269:Ribosomal Proteins / Q000378:metabolism; D013002:Somatomedins / Q000502:physiology; D015533:Transcriptional Activation
chemical_list: D029742:Caenorhabditis elegans Proteins; D007328:Insulin; C000593318:NOG-1 protein, C elegans; D012269:Ribosomal Proteins; D013002:Somatomedins; D020558:GTP Phosphohydrolases