title: Expression of Potential Regulatory Genes in Abdominal Adipose Tissue of Broiler Chickens during Early Development.
abstract: The identities of genes that underlie population variation in adipose tissue development in farm animals are poorly understood. Previous studies in our laboratory have suggested that increased fat tissue involves the expression modulation of an array of genes in broiler chickens. Of special interest are eight genes, FGFR3, EPHB2, IGFBP2, GREM1, TNC, COL3A1, ACBD7, and SCD. To understand their expression regulation and response to dietary manipulation, we investigated their mRNA levels after dietary manipulation during early development. Chickens were fed either a recommended standard or a high caloric diet from hatch to eight weeks of age (WOA). The high caloric diet markedly affected bodyweight of the broiler birds. mRNA levels of the eight genes in the abdominal adipose tissue were assayed at 2, 4, 6, and 8 WOA using RT-qPCR. Results indicate that (1) FGFR3 mRNA level was affected significantly by diet, age, and diet:age interaction; (2) COL3A mRNA level was repressed by high caloric diet; (3) mRNA levels of EPHB2, ACBD7, and SCD were affected by age; (4) mRNA level of TNC was modulated by age:diet interaction; (5) changes in GREM1 and IGFBP2 mRNA levels were not statistically different.
authors.affiliation: College of Agriculture, Human and Natural Science, Tennessee State University, Nashville, TN 37209, USA.
authors.forename: Ann
authors.initials: A
authors.lastname: Bohannon-Stewart
authors.affiliation: College of Agriculture, Human and Natural Science, Tennessee State University, Nashville, TN 37209, USA.
authors.forename: Gary
authors.identifier: 0000-0002-7718-5395
authors.initials: G
authors.lastname: Kelley
authors.affiliation: College of Agriculture, Human and Natural Science, Tennessee State University, Nashville, TN 37209, USA.
authors.forename: Boniface
authors.identifier: 0000-0002-9386-366X
authors.initials: B
authors.lastname: Kimathi
authors.affiliation: College of Agriculture, Human and Natural Science, Tennessee State University, Nashville, TN 37209, USA.
authors.forename: Sri Harsha K V
authors.initials: SH
authors.lastname: Subramanya
authors.affiliation: College of Agriculture, Human and Natural Science, Tennessee State University, Nashville, TN 37209, USA.
authors.forename: Joseph
authors.initials: J
authors.lastname: Donkor
authors.affiliation: College of Agriculture, Human and Natural Science, Tennessee State University, Nashville, TN 37209, USA.
authors.forename: Carl
authors.identifier: 0000-0002-1320-7612
authors.initials: C
authors.lastname: Darris
authors.affiliation: College of Agriculture, Human and Natural Science, Tennessee State University, Nashville, TN 37209, USA.
authors.forename: James
authors.initials: J
authors.lastname: Tyus
authors.affiliation: College of Agriculture, Human and Natural Science, Tennessee State University, Nashville, TN 37209, USA.
authors.forename: Ashley
authors.identifier: 0000-0002-7925-6230
authors.initials: A
authors.lastname: Payne
authors.affiliation: College of Agriculture, Human and Natural Science, Tennessee State University, Nashville, TN 37209, USA.
authors.forename: Shannon
authors.identifier: 0000-0002-3352-7808
authors.initials: S
authors.lastname: Byers
authors.affiliation: College of Agriculture, Human and Natural Science, Tennessee State University, Nashville, TN 37209, USA.
authors.forename: Dafeng
authors.initials: D
authors.lastname: Hui
authors.affiliation: College of Agriculture, Human and Natural Science, Tennessee State University, Nashville, TN 37209, USA.
authors.forename: Samuel
authors.identifier: 0000-0003-3985-3281
authors.initials: S
authors.lastname: Nahashon
authors.affiliation: College of Agriculture, Human and Natural Science, Tennessee State University, Nashville, TN 37209, USA.
authors.forename: Fur-Chi
authors.identifier: 0000-0001-9447-372X
authors.initials: FC
authors.lastname: Chen
authors.affiliation: College of Agriculture, Human and Natural Science, Tennessee State University, Nashville, TN 37209, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Ivy
authors.affiliation: College of Agriculture, Human and Natural Science, Tennessee State University, Nashville, TN 37209, USA ; Department of Biological Sciences, Tennessee State University, 3500 John A. Merritt Bou Levard, Nashville, TN 37209, USA.
authors.forename: Xiaofei
authors.identifier: 0000-0003-3832-5217
authors.initials: X
authors.lastname: Wang