title: Histopathologycal findings in the ovaries and uterus of albino female rats promoted by co-administration of synthetic steroids and nicotine.
abstract: The use of anabolic androgenic steroids is often associated with the use of other substances, licit or not, such as nicotine present in the tobacco. The present study investigated for the first time the effects of co-administration of synthetic steroids and nicotine on the ovarian and uterine tissue and fertility of adult female rats. Animals were submitted to treatment groups (n=16/group): nandrolone decanoate (ND; 7.5mg/kg BW/week); testosterone mixture (T; 7.5mg/kg BW/week); nicotine (N; 2.0mg/kg BW/day), and co-administration of ND/N, T/N and ND/T/N. The control group received saline solution daily. The injections were administered subcutaneously for 30 consecutive days. Results demonstrated that all androgenized rats exhibited estral acyclicity and there was suppression of reproductive capacity due to notable ovarian and uterine histological changes. Treatments promoted decrease (p<0.05) in the ovarian weight. Uterine weight increased (p<0.05) in the T and T/N groups, in comparison to control group. ND or T co-administered or not to nicotine promoted intense follicular degeneration, with formation of cysts in the ovaries. High levels of circulating androgens in the ND/T/N group induced the presence of ovarian sex cord-stromal tumors of Sertoli cell pattern. Androgenized females presented endometrial changes characterized by papilliferous or pleated luminal epithelium, oedematous and hemorrhagic stroma and presence of gland cysts. In conclusion, the co-administration of three drugs promoted atypical morphological pattern on the ovaries and uterus of female rats.
authors.affiliation: Univ Estadual PaulistaUNESP, Biological Sciences Department, Av. Dom Antonio, 2100, Campus Universitário, 19806-900 Assis, SP, Brazil. Electronic address: camargo@assis.unesp.br.
authors.forename: Isabel Cristina Cherici
authors.initials: IC
authors.lastname: Camargo
authors.affiliation: Univ Estadual PaulistaUNESP, Biological Sciences Department, Av. Dom Antonio, 2100, Campus Universitário, 19806-900 Assis, SP, Brazil.
authors.forename: Gabriel Adan Araújo
authors.initials: GA
authors.lastname: Leite
authors.affiliation: Univ Estadual PaulistaUNESP, Biological Sciences Department, Av. Dom Antonio, 2100, Campus Universitário, 19806-900 Assis, SP, Brazil.
authors.forename: Tiago
authors.initials: T
authors.lastname: Pinto
authors.affiliation: Univ Estadual PaulistaUNESP, Biological Sciences Department, Av. Dom Antonio, 2100, Campus Universitário, 19806-900 Assis, SP, Brazil.
authors.forename: João Tadeu
authors.initials: JT
authors.lastname: Ribeiro-Paes
mesh_terms: D045930:Anabolic Agents / Q000008:administration & dosage / Q000009:adverse effects*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004357:Drug Synergism; D004952:Esters; D030762:Estrous Cycle / Q000187:drug effects; D005260:Female; D005298:Fertility / Q000187:drug effects; D007279:Injections, Subcutaneous; D009277:Nandrolone / Q000008:administration & dosage / Q000009:adverse effects / Q000031:analogs & derivatives*; D000077603:Nandrolone Decanoate; D009538:Nicotine / Q000008:administration & dosage / Q000009:adverse effects*; D009929:Organ Size / Q000187:drug effects; D010053:Ovary / Q000187:drug effects / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013739:Testosterone / Q000008:administration & dosage / Q000009:adverse effects*; D014599:Uterus / Q000187:drug effects / Q000473:pathology
chemical_list: D045930:Anabolic Agents; D004952:Esters; D013739:Testosterone; D009538:Nicotine; D009277:Nandrolone; D000077603:Nandrolone Decanoate
keywords: Histopathology; Nandrolone decanoate; Nicotine; Ovary; Rats; Testosterone mixture; Uterus