title: Photothermal spectral-domain optical coherence reflectometry for direct measurement of hemoglobin concentration of erythrocytes.
abstract: A novel optical detection method for hemoglobin concentration is described. The hemoglobin molecules consisting mainly of iron generate heat upon their absorption of light energy at 532 nm, which subsequently changes the refractive index of the blood. We exploit this photothermal effect to determine the hemoglobin concentration of erythrocytes without any preprocessing of blood. Highly sensitive measurement of refractive index alteration of blood samples is enabled by a spectral-domain low coherence reflectometric sensor with subnanometer-level optical path-length sensitivity. The performance and validity of the sensor are presented by comparing the measured results against the reference data acquired from an automatic hematology analyzer.
authors.affiliation: School of Mechanical Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea.
authors.forename: Jinyeong
authors.initials: J
authors.lastname: Yim
authors.affiliation: School of Mechanical Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea.
authors.forename: Hun
authors.initials: H
authors.lastname: Kim
authors.affiliation: School of Mechanical Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea.
authors.forename: Suho
authors.initials: S
authors.lastname: Ryu
authors.affiliation: Department of Laboratory Medicine, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea.
authors.forename: Sungwook
authors.initials: S
authors.lastname: Song
authors.affiliation: Department of Laboratory Medicine, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea.
authors.forename: Hyun Ok
authors.initials: HO
authors.lastname: Kim
authors.affiliation: School of Mechanical Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea.
authors.forename: Kyung-A
authors.initials: KA
authors.lastname: Hyun
authors.affiliation: School of Mechanical Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea.
authors.forename: Hyo-Il
authors.initials: HI
authors.lastname: Jung
authors.affiliation: School of Mechanical Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea. Electronic address: cjoo@yonsei.ac.kr.
authors.forename: Chulmin
authors.initials: C
authors.lastname: Joo
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D004912:Erythrocytes / Q000737:chemistry*; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D007368:Interferometry / Q000295:instrumentation*; D008027:Light; D057230:Limit of Detection; D012031:Refractometry / Q000295:instrumentation*; D013696:Temperature
chemical_list: D006454:Hemoglobins
keywords: Erythrocytes; Hemoglobin concentration; Low coherence interferometry; Photothermal effect; Refractive index