title: Preclinical studies with JAA-F11 anti-Thomsen-Friedenreich monoclonal antibody for human breast cancer.
abstract: OBJECTIVE
  The Thomsen-Friedenreich antigen (TF-Ag) is a disaccharide hidden on normal cells, but selectively exposed on the surface of breast, colon, prostate and bladder cancer cells. JAA-F11, a highly specific monoclonal antibody to TF-Ag, reduces metastasis and prolongs survival in a mouse model. In addition,(124)I-JAA-F11 localizes 4T1 tumors in mice. These studies continue translation of JAA-F11 to human breast cancer. MATERIALS & METHODS & RESULTS: Of the 41 human breast cancer cell lines tested, 78% were positive for reactivity with JAA-F11 by whole-cell enzyme immunoassay and positivity occurred unrelated to estrogen, progesterone or HER2 receptor status. JAA-F11 inhibited the growth rate of the human cancer cell lines tested. At 1 h, approximately 80% of JAA-F11 internalized in the three cell lines tested. (124)I-JAA-F11 specifically imaged human triple-negative tumors in mice by microPET.
  
  
  CONCLUSIONS
  The results highlight the potential that humanized JAA-F11 may have for immunotherapy and drug conjugate therapy in breast cancer patients.
authors.affiliation: Department of Biotechnical & Clinical Laboratory Sciences, University of Buffalo, Buffalo, NY 14214, USA.
authors.forename: Kimiko
authors.initials: K
authors.lastname: Ferguson
authors.forename: Arti
authors.initials: A
authors.lastname: Yadav
authors.forename: Susan
authors.initials: S
authors.lastname: Morey
authors.forename: Julia
authors.initials: J
authors.lastname: Abdullah
authors.forename: Gabriel
authors.initials: G
authors.lastname: Hrysenko
authors.forename: Jing Ying
authors.initials: JY
authors.lastname: Eng
authors.forename: Munawwar
authors.initials: M
authors.lastname: Sajjad
authors.forename: Stephen
authors.initials: S
authors.lastname: Koury
authors.forename: Kate
authors.initials: K
authors.lastname: Rittenhouse-Olson
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D000970:Antineoplastic Agents / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D016513:Mice, SCID; D064726:Triple Negative Breast Neoplasms / Q000188:drug therapy*; D023041:Xenograft Model Antitumor Assays
chemical_list: D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents