title: Fabrication and neuron cytocompatibility of iron oxide nanoparticles coated with silk-fibroin peptides.
abstract: Neuro-cytotoxicity of iron oxide nanoparticles (NPs) need to be considered. In this paper, magnetic Fe3O4 NPs are synthesized via a bio-mineralization process using silk fibroin (SF) as template. The prepared NPs with SF (SF-NPs) have more uniform grain size, better stability in weakly alkaline solution and higher saturation magnetization (about 82 emu/g) than pure NPs. After exposure to different concentration of SF-NPs (6.25-100 μg/mL), the reactive oxygen species generation in PC12 cells reduced compared with pure NPs. 1-5d treatment with SF-NPs did not destroy cell membrane and cyto-skeleton, and could improve the neurites extension in a dose-dependent manner at lower concentration (6.25-50 μg/mL), because SF peptide coating could delay the release of iron ions and the increase of surface crystal defects of NPs. Intact mitochondria in a neurite indicate the extension activity of neurites of cells treated with SF-NPs. Their high magnetic property and good neural-cytocompatibility provide the possibility of SF-NPs to be used as the contrast agents in brain MRI or the carrier of neural therapeutic drugs.
authors.affiliation: College of Materials Science and Engineering, Sichuan University, Chengdu 610065, China.
authors.forename: Min
authors.initials: M
authors.lastname: Deng
authors.affiliation: College of Materials Science and Engineering, Sichuan University, Chengdu 610065, China. Electronic address: zbhuang@scu.edu.cn.
authors.forename: Zhongbing
authors.initials: Z
authors.lastname: Huang
authors.affiliation: College of Materials Science and Engineering, Sichuan University, Chengdu 610065, China. Electronic address: zouyuanwen@aliyun.com.
authors.forename: Yuanwen
authors.initials: Y
authors.lastname: Zou
authors.affiliation: College of Materials Science and Engineering, Sichuan University, Chengdu 610065, China.
authors.forename: Guangfu
authors.initials: G
authors.lastname: Yin
authors.affiliation: College of Materials Science and Engineering, Sichuan University, Chengdu 610065, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
authors.affiliation: The Military General Hospital of Chengdu, PLA, Tianhui Town, Chengdu 610083, China.
authors.forename: Jianwen
authors.initials: J
authors.lastname: Gu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D005349:Fibroins / Q000737:chemistry* / Q000378:metabolism; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D009474:Neurons / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D016716:PC12 Cells; D010316:Particle Size; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D047011:Silk / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D058185:Magnetite Nanoparticles; D010455:Peptides; D017382:Reactive Oxygen Species; D047011:Silk; D005349:Fibroins
keywords: Iron ion release; Iron oxide nanoparticles; Neuro-cytocompatibility; Reactive oxygen species; Silk fibroin coating