title: Determination of amino acids by microemulsion electrokinetic chromatography laser induced fluorescence method.
abstract: In this study, detection of 20 FITC-derivatized amino acids using an MEEKC-LIF was demonstrated. In order to achieve good separation for hydrophobic amino acids, the MEEKC method was employed and detection limits were obtained in the range of 0.32-2.2 nM, which is comparable to previous reports on amino acid analyses. Furthermore, a significant reduction in the reaction time from 1 h for conventional derivatization to 3 min for the microwave-assisted derivatization was observed and achieved, as opposed to the traditional pretreatment of real sample due to its complexity prior to the analysis of amino acid content. Finally, this microwave-assisted derivatization MEEKC-LIF method successfully determined amino acids in beverage, food, and biological samples (rat brain) with good recovery.
authors.affiliation: Center for Nanotechnology, Department of Chemistry, Chung Yuan Christian University, Chung-Li, Taiwan.
authors.forename: Wei-Cheng
authors.initials: WC
authors.lastname: Lin
authors.forename: Wan-Ling
authors.initials: WL
authors.lastname: Liu
authors.forename: Wei-Ying
authors.initials: WY
authors.lastname: Tang
authors.forename: Chin-Ping
authors.initials: CP
authors.lastname: Huang
authors.forename: Hsi-Ya
authors.initials: HY
authors.lastname: Huang
authors.forename: Ting-Yu
authors.initials: TY
authors.lastname: Chin
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D001628:Beverages / Q000032:analysis*; D001923:Brain Chemistry; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004655:Emulsions / Q000737:chemistry; D016650:Fluorescein-5-isothiocyanate / Q000032:analysis; D005453:Fluorescence; D007834:Lasers; D057230:Limit of Detection; D008297:Male; D008872:Microwaves; D051381:Rats; D013050:Spectrometry, Fluorescence
chemical_list: D000596:Amino Acids; D004655:Emulsions; D016650:Fluorescein-5-isothiocyanate
keywords: Amino acid; Capillary electrophoresis; Laser-induced fluorescence; Microemulsion electrokinetic chromatography; Microwave-assisted derivatization