title: Robustness of a method based on the serial coupling of reversed-phase and zwitterionic hydrophilic interaction LC-MS for the analysis of phenols.
abstract: We have recently proved that the serial coupling of RP and zwitterionic hydrophilic interaction LC with mass spectrometric detection is a versatile and reliable technique to simultaneously separate polar and apolar compounds in complex samples, for example, phenols in wine. In order to evaluate the system suitability for long-term usage, the robustness of a method based on the serial coupling of RP and zwitterionic hydrophilic interaction LC was evaluated after one year of analyses comprising >1100 injections. The retention time and peak shape of phenol standards and phenols in a red wine were compared to the values previously published. Phenol retention times were shifted <30 s. However, the peak widths were increased, partially due to the deterioration of the stationary phases. Nevertheless, the method was still highly reliable for the analysis of phenols in wine.
authors.affiliation: Chair of Urban Water Systems Engineering, Technische Universitaet Muenchen, Garching, Germany.
authors.forename: Giorgia
authors.initials: G
authors.lastname: Greco
authors.forename: Sylvia
authors.initials: S
authors.lastname: Grosse
authors.forename: Thomas
authors.initials: T
authors.lastname: Letzel
mesh_terms: D002851:Chromatography, High Pressure Liquid; D057927:Hydrophobic and Hydrophilic Interactions; D013058:Mass Spectrometry; D010636:Phenols / Q000032:analysis* / Q000737:chemistry*; D014920:Wine / Q000032:analysis
chemical_list: D010636:Phenols
keywords: Hydrophilic interaction liquid chromatography; Phenols; Robustness; Serial coupling; Wine