title: A rapid and improved technique for scanning electron microscopy of actinomycetes.
abstract: A simple and rapid method for the preparation of actinomycete cultures before scanning electron microscopy (SEM) is reported. Streptomyces strains were grown in starch casein broth with sterile glass wool as support. The cultures were dehydrated by lyophilization eliminating the use of chemical fixatives and dehydrating agents.
authors.affiliation: Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, Dr. D. Y. Patil Vidyapeeth, Pune 411033, India.
authors.forename: Divya
authors.initials: D
authors.lastname: Prakash
authors.affiliation: Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, Dr. D. Y. Patil Vidyapeeth, Pune 411033, India. Electronic address: neelunawani@yahoo.com.
authors.forename: N N
authors.initials: NN
authors.lastname: Nawani
mesh_terms: D039903:Actinobacteria / Q000648:ultrastructure*; D005612:Freeze Drying / Q000379:methods*; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D013997:Time Factors
keywords: Actinomycetes; Lyophilization; Scanning electron microscopy