title: Characterization and biocontrol potential of entomopathogenic fungus, Beauveria bassiana isolates against Spilarctia obliqua.
abstract: Beauveria bassiana is a known natural enemy of a number of insect pests of crop plants. In order to screen different isolates of any given entomopathogens molecular markers provide a means for constructing the molecular phylogeny, diversity and link to virulent phenotypes. Eight isolates of B. bossiona isolated from different insect hosts and from different location at Pantnagar (Uttrakhand) were characterized by PCR-based RAPD markers. Bioassays were conducted by using first, second and third instar larvae of Spilarctia oblique in order to categorize the isolates based on virulence. The isolates were arbitrarily rated as more virulent, moderately virulent and less virulent based on the speed of killing. A wide range of variation in virulence was observed and the isolates of same insect origin and location showed differences in their aggressiveness. No correlation was found between the pathogenicity of the isolates and the relatedness of the original insect host. The pathogenicity against first, second and third instar larva of Spilarctia obliqua did not reveal any relatedness with the clustering pattern.
authors.affiliation: Department of Plant Pathology, GB. Pant University ofAgriculture and Technology, Pantnagar-263 145, India. bpbhadauria@rediffmail.com
authors.forename: B P
authors.initials: BP
authors.lastname: Bhadauria
authors.affiliation: Department of Entomology, RBS College, Bichpuri, Dr. B. R. Ambedkar University, Agra-283 105, India.
authors.forename: P K
authors.initials: PK
authors.lastname: Singh
authors.affiliation: Department of Forest Protection, Rain Forest Research Institute, Jorhat-785 001, India.
authors.forename: Pandey
authors.initials: P
authors.lastname: Shailesh
authors.affiliation: NASC Complex, IRRI Office, New Delhi-110 012, India.
authors.forename: N W
authors.initials: NW
authors.lastname: Zaidi
authors.affiliation: NASC Complex, IRRI Office, New Delhi-110 012, India.
authors.forename: U S
authors.initials: US
authors.lastname: Singh
mesh_terms: D000818:Animals; D052982:Beauveria / Q000235:genetics / Q000502:physiology*; D061046:Biological Control Agents; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D007814:Larva / Q000254:growth & development / Q000382:microbiology; D009036:Moths / Q000254:growth & development / Q000382:microbiology*; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D061046:Biological Control Agents; D005656:Fungal Proteins