title: Bone metabolism, osteoprotegerin, receptor activator of nuclear factor-κB ligand and selected adipose tissue hormones in girls with anorexia nervosa.
abstract: BACKGROUND
  The aim of this study was to determine whether girls with anorexia nervosa (AN) exhibited any relationships between serum levels of LP, ADIPO, RES, VISF, APE-36, APE-12, and bone markers, OPG and sRANKL.
  
  
  METHODS
  Serum levels of selected adipose tissue hormones, OC, CTx, OPG and sRANKL were assessed using ELISA in 86 study participants suffering from AN and 21 healthy controls, all aged 13 to 18 years.
  
  
  RESULTS
  Girls with AN showed a significant reduction in body mass, BMI, serum concentrations of LP, RES, VISF, APE-36, APE-12, OC, CTx and increased ADIPO concentration. These changes were associated with significant increases in OPG and sRANKL and a decrease in the OPG/sRANKL ratio. Significant positive correlations were revealed between BMI and LP, APE-36, CTx, OPG/sRANKL ratio; OC and VISF; OPG and ADIPO; OPG/sRANKL ratio and LP, APE-36, APE-12. Significant negative correlations were revealed between CTx, sRANKL and RES, APE-36; OPG and APE-36, APE-12; OPG/sRANKL ratio and ADIPO. VISF was shown to be an independent predictor of OC. APE-36 and RES turned out to be independent predictors of CTx, and sRANKL, APE-36 and ADIPO were independent predictors of OPG while APE-36, LP and ADIPO were independent predictors of the OPG/sRANKL ratio.
  
  
  CONCLUSIONS
  Changes in bone markers, OPG, sRANKL and/or the OPG/sRANKL ratio exhibited by girls with AN have been found to be associated with changes in the levels of the selected adipose tissue hormones. Abnormal relationships between bone metabolism and LP, ADIPO, RES, VISF and APE might adversely affect the balance of the OPG/sRANKL system and thus potentially compromise the mechanism which compensates for bone remodelling disturbances.
authors.affiliation: ozdrasiek@wp.pl.
authors.forename: Zofia
authors.initials: Z
authors.lastname: Ostrowska
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Ziora
authors.forename: Joanna
authors.initials: J
authors.lastname: Oświęcimska
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Świętochowska
authors.forename: Bogdan
authors.initials: B
authors.lastname: Marek
authors.forename: Dariusz
authors.initials: D
authors.lastname: Kajdaniuk
authors.forename: Kinga
authors.initials: K
authors.lastname: Wołkowska-Pokrywa
authors.forename: Beata
authors.initials: B
authors.lastname: Kos-Kudła
mesh_terms: D052242:Adiponectin / Q000097:blood; D000273:Adipose Tissue / Q000378:metabolism; D000293:Adolescent; D000856:Anorexia Nervosa / Q000097:blood*; D000073861:Apelin; D015415:Biomarkers / Q000097:blood; D001842:Bone and Bones / Q000378:metabolism; D005260:Female; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000097:blood; D020738:Leptin / Q000097:blood; D053244:Osteoprotegerin / Q000097:blood*; D053245:RANK Ligand / Q000097:blood*; D052243:Resistin / Q000097:blood
chemical_list: C115436:APLN protein, human; D052242:Adiponectin; D000073861:Apelin; D015415:Biomarkers; D036341:Intercellular Signaling Peptides and Proteins; D020738:Leptin; D053244:Osteoprotegerin; D053245:RANK Ligand; D052243:Resistin; C495200:apelin-12 peptide