title: Identification of a tumor-suppressive human-specific microRNA within the FHIT tumor-suppressor gene.
abstract: Loss or attenuated expression of the tumor-suppressor gene FHIT is associated paradoxically with poor progression of human tumors. Fhit promotes apoptosis and regulates reactive oxygen species; however, the mechanism by which Fhit inhibits tumor growth in animals remains unclear. In this study, we used a multidisciplinary approach based on bioinformatics, small RNA library screening, human tissue analysis, and a xenograft mouse model to identify a novel member of the miR-548 family in the fourth intron of the human FHIT gene. Characterization of this human-specific microRNA illustrates the importance of this class of microRNAs in tumor suppression and may influence interpretation of Fhit action in human cancer.
authors.affiliation: Authors' Affiliations: Department of Medical Molecular Biology, Beijing Institute of Biotechnology; Center of Computational Biology, Beijing Institute of Basic Medical Sciences; Department of Pathology, Chinese PLA General Hospital; Department of Biochemistry and Molecular Biology, Beijing Institute of Radiation Medicine, Beijing, China; Department of Radiation Oncology, Emory University School of Medicine, Winship Cancer Institute of Emory University; School of Biology, Georgia Institute of Technology, Atlanta, Georgia; Department of Cancer Biology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania; and Genome Institute, National Center for Genetic Engineering and Biotechnology, Pathumthani, Thailand.
authors.forename: Baocheng
authors.initials: B
authors.lastname: Hu
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Ying
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Jittima
authors.initials: J
authors.lastname: Piriyapongsa
authors.forename: I King
authors.initials: IK
authors.lastname: Jordan
authors.forename: Jipo
authors.initials: J
authors.lastname: Sheng
authors.forename: Fang
authors.initials: F
authors.lastname: Yu
authors.forename: Po
authors.initials: P
authors.lastname: Zhao
authors.forename: Yazhuo
authors.initials: Y
authors.lastname: Li
authors.forename: Hongyan
authors.initials: H
authors.lastname: Wang
authors.forename: Wooi Loon
authors.initials: WL
authors.lastname: Ng
authors.forename: Shuofeng
authors.initials: S
authors.lastname: Hu
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
authors.forename: Chenguang
authors.initials: C
authors.lastname: Wang
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Zheng
authors.forename: Wuju
authors.initials: W
authors.lastname: Li
authors.forename: Walter J
authors.initials: WJ
authors.lastname: Curran
authors.forename: Ya
authors.initials: Y
authors.lastname: Wang
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000235:genetics*; D000818:Animals; D045744:Cell Line, Tumor; D016147:Genes, Tumor Suppressor; D057809:HEK293 Cells; D006367:HeLa Cells; D064593:Heterografts; D006801:Humans; D007438:Introns; D008297:Male; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D009363:Neoplasm Proteins / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection; D000099170:Bis(5'-adenosyl)triphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D035683:MicroRNAs; D009363:Neoplasm Proteins; D000099170:Bis(5'-adenosyl)triphosphatase; C561887:MIRN548 microRNA, human