title: New triterpenoid saponins from the roots of Saponaria officinalis.
abstract: Three new triterpenoid saponins (1-3), along with nine known saponins, were isolated from the roots of Saponaria officinalis L. Two of them: vaccaroside D (4) and dianchinenoside B (5) are known, but not previously reported for S. officinalis, and seven others: saponarioside C (6), D (7), F (8), G (9), I (10), K (11), and L (12) have been previously isolated from this plant. The structures of the new saponins were established as 3-O-beta-D-xylopyranosyl-16alpha-hydroxygypsogenic acid-28-O-[beta-D-glucopyranosyl-(1 -->6)-beta-D-glucopyranoside (1), 3-O-beta-D-xylopyranosyl-16alpha-hydroxygypsogenic acid-28-O-[beta-D-glucopyranosyl-(1-->3)]-[alpha-D-galactopyranosyl-(1-->6)-alpha-D-galactopyranosyl-(1-->6)-beta-D-glucopyranosyl-(1-->6)]-beta-D-glucopyranoside (2) and 3-O-beta-D-xylopyranosyl-gypsogenic acid-28-O-[beta-D-glucopyranosyl-(1-->3)]-[6-O-(3-hydroxy-3-methylglutaryl)-beta-D-glucopyranosyl-(1-->6)]-beta-D-glucopyranoside (3). Their structures were elucidated by extensive spectroscopic methods, including 1D- (1H, 13C) and 2D-NMR (D QF-COSY, TOCSY, ROESY, HSQC and HMBC) experiments, as well as high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), ESI-MS/MS and acid hydrolysis.
authors.affiliation: Department of Biochemistry, Institute of Soil Science and Plant Cultivation, State Research Institute, Czartoryskich 8, 24-100 Pulawy, Poland. bszajwaj@iung.pulawy.pl
authors.forename: Barbara
authors.initials: B
authors.lastname: Moniuszko-Szajwaj
authors.affiliation: Department of Biochemistry, Institute of Soil Science and Plant Cultivation, State Research Institute, Czartoryskich 8, 24-100 Pulawy, Poland.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Pecio
authors.affiliation: Department of Biochemistry, Institute of Soil Science and Plant Cultivation, State Research Institute, Czartoryskich 8, 24-100 Pulawy, Poland.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Kowalczyk
authors.affiliation: Grupo de Alelopatia, Departamento de Quimica Organica, Facultad de Ciencias, Universidad de Cadiz, C/Republica Saharaui, s/n, 11510 Puerto Real, Cadiz, Spain.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Simonet
authors.affiliation: Grupo de Alelopatia, Departamento de Quimica Organica, Facultad de Ciencias, Universidad de Cadiz, C/Republica Saharaui, s/n, 11510 Puerto Real, Cadiz, Spain.
authors.forename: Francisco A
authors.initials: FA
authors.lastname: Macias
authors.affiliation: Department of Animal Nutrition and Feed Management, RUMEN PULS, Poznan University of Life Sciences, Wolyńska 33, 60-637 Poznań, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Szumacher-Strabel
authors.affiliation: Department of Animal Nutrition and Feed Management, RUMEN PULS, Poznan University of Life Sciences, Wolyńska 33, 60-637 Poznań, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Cieslak
authors.affiliation: Department of Biochemistry, Institute of Soil Science and Plant Cultivation, State Research Institute, Czartoryskich 8, 24-100 Pulawy, Poland.
authors.forename: Wieslaw
authors.initials: W
authors.lastname: Oleszek
authors.affiliation: Department of Biochemistry, Institute of Soil Science and Plant Cultivation, State Research Institute, Czartoryskich 8, 24-100 Pulawy, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Stochmal
mesh_terms: D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D029750:Saponaria / Q000737:chemistry*; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D014315:Triterpenes