title: The effect of fluoride toothpaste on root dentine demineralization progression: a pilot study.
abstract: The anticaries effect of fluoride (F) toothpaste containing 1100 µg F/g in reducing enamel demineralization is well established, but its effect on dentine has not been extensively studied. Furthermore, it has been shown that toothpaste containing a high F concentration is necessary to remineralize root dentine lesions, suggesting that a 1100 µg F/g concentration might not be high enough to reduce root dentine demineralization, particularly when dentine is subjected to a high cariogenic challenge. Thus, the aim of this pilot study was to evaluate in situ the effect of F toothpaste, at a concentration of 1100 µg F/g, on dentine demineralization. In a crossover and double-blind study, conducted in two phases of 14 days, six volunteers wore a palatal appliance containing four slabs of bovine root dentine whose surface hardness (SH) was previously determined and to which a 10% sucrose solution was applied extra-orally 8×/day. Volunteers used a non-F toothpaste (negative control) or F toothpaste (1100 µg F/g, NaF/SiO2) three times a day. On the 10th and 14th days of each phase, two slabs were collected and SH was determined again. Dentine demineralization was assessed as percentage of SH loss (%SHL). The effect of toothpaste was significant, showing lower %SHL for the F toothpaste group (42.0 ± 9.7) compared to the non-F group (62.0 ± 6.4; p < 0.0001), but the effect of time was not significant (p > 0.05). This pilot study suggests that F toothpaste at 1100 µg F/g is able to decrease dentine caries even under a high cariogenic challenge of biofilm accumulation and sugar exposure.
authors.affiliation: Department of Physiological Sciences, Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil.
authors.forename: Juliana Nunes
authors.initials: JN
authors.lastname: Botelho
authors.affiliation: Department of Periodontology and Prosthodontics, Piracicaba Dental School, Department of Periodontology and Prosthodontics, Piracicaba, SP, Brazil.
authors.forename: Altair Antoninha
authors.initials: AA
authors.lastname: Del Bel Cury
authors.affiliation: Department of Periodontology and Prosthodontics, Piracicaba Dental School, Department of Periodontology and Prosthodontics, Piracicaba, SP, Brazil.
authors.forename: Wander Jose da
authors.initials: WJ
authors.lastname: Silva
authors.affiliation: Department of Physiological Sciences, Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil.
authors.forename: Livia Maria Andalo
authors.initials: LM
authors.lastname: Tenuta
authors.affiliation: Department of Physiological Sciences, Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil.
authors.forename: Jaime Aparecido
authors.initials: JA
authors.lastname: Cury
mesh_terms: D000328:Adult; D000818:Animals; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D002327:Cariostatic Agents / Q000008:administration & dosage*; D016022:Case-Control Studies; D002417:Cattle; D018592:Cross-Over Studies; D003731:Dental Caries / Q000517:prevention & control; D019422:Dietary Sucrose / Q000009:adverse effects; D018450:Disease Progression; D004311:Double-Blind Method; D005459:Fluorides / Q000008:administration & dosage*; D006801:Humans; D010865:Pilot Projects; D017001:Tooth Demineralization / Q000188:drug therapy*; D014092:Tooth Root / Q000187:drug effects; D014100:Toothpastes / Q000008:administration & dosage*; D055815:Young Adult
chemical_list: D002327:Cariostatic Agents; D019422:Dietary Sucrose; D014100:Toothpastes; D005459:Fluorides