title: Alkali burn versus suture-induced corneal neovascularization in C57BL/6 mice: an overview of two common animal models of corneal neovascularization.
abstract: The purpose of the present study was to quantify and compare corneal hem- and lymphangiogenesis between alkali burn and suture-induced corneal neovascularization (CNV) in two commonly used mouse strains. A retrospective analysis was performed on C57BL/6 and FVB neovascularized corneas. CNV was induced by surface caustication with NaOH or intrastromal placement of three 10.0 nylon sutures. Hemangiogenesis and lymphangiogenesis extent was calculated on whole mounted corneas by CD31 and LYVE1 immunofluorescence analysis. Blood vessel growth was similar between alkali burn and suture-induced CNV in C57BL/6 mice, and between C57BL/6 and FVB sutured strains. On the contrary, corneal lymphangiogenesis was more pronounced in the C57BL/6 sutured mice versus the alkali burn group, and in the FVB strain versus both C57BL/6 models. These results indicate that significant differences occur in lymphangiogenesis, but not hemangiogenesis, in the alkali burn and suture-induced models in C57BL/6 mice. Furthermore, lymphangiogenesis is more pronounced in the albino (FVB) strain after suture placement. We suggest that the suture model has a number of advantages and may be preferentially used to study corneal lymphangiogenesis.
authors.affiliation: San Raffaele Scientific Institute, Via Olgettina 60, 20132 Milan, Italy. Electronic address: giacomini.chiara@hsr.it.
authors.forename: Chiara
authors.initials: C
authors.lastname: Giacomini
authors.affiliation: San Raffaele Scientific Institute, Via Olgettina 60, 20132 Milan, Italy; San Raffaele Hospital, Cornea and Ocular Surface Unit, Via Olgettina 60, 20132 Milan, Italy. Electronic address: ferrari.giulio@hsr.it.
authors.forename: Giulio
authors.initials: G
authors.lastname: Ferrari
authors.affiliation: San Raffaele Scientific Institute, Via Olgettina 60, 20132 Milan, Italy. Electronic address: bignami.fabio@hsr.it.
authors.forename: Fabio
authors.initials: F
authors.lastname: Bignami
authors.affiliation: San Raffaele Scientific Institute, Via Olgettina 60, 20132 Milan, Italy; San Raffaele Hospital, Cornea and Ocular Surface Unit, Via Olgettina 60, 20132 Milan, Italy. Electronic address: rama.paolo@hsr.it.
authors.forename: Paolo
authors.initials: P
authors.lastname: Rama
mesh_terms: D000818:Animals; D002057:Burns, Chemical / Q000150:complications*; D016510:Corneal Neovascularization / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D003319:Corneal Stroma / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D005126:Eye Burns / Q000139:chemically induced*; D019084:Fluorescent Antibody Technique, Indirect; D006023:Glycoproteins / Q000378:metabolism; D042583:Lymphangiogenesis; D026901:Membrane Transport Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D009757:Nylons; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D012189:Retrospective Studies; D012972:Sodium Hydroxide / Q000633:toxicity; D013537:Sutures / Q000009:adverse effects*; D013997:Time Factors
chemical_list: D006023:Glycoproteins; D026901:Membrane Transport Proteins; D009757:Nylons; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D012972:Sodium Hydroxide; C492273:LYVE1 protein, mouse
keywords: alkali burn; cornea; lymphangiogenesis; neovascularization; suture