title: Model based analysis of transient fluorescence yield induced by actinic laser flashes in spinach leaves and cells of green alga Chlorella pyrenoidosa Chick.
abstract: Measurements of Single Flash Induced Transient Fluorescence Yield (SFITFY) on spinach leaves and whole cells of green thermophilic alga Chlorella pyrenoidosa Chick were analyzed for electron transfer (ET) steps and coupled proton transfer (PT) on both the donor and the acceptor side of the reaction center (RC) of photosystem II (PS II). A specially developed PS II model (Belyaeva et al., 2008, 2011a) allowed the determination of ET steps that occur in a hierarchically ordered time scale from nanoseconds to several seconds. Our study demonstrates that our SFITFY data is consistent with the concept of the reduction of P680(+) by YZ in both leaves and algae (studied on spinach leaves and cells of Chlorella pyrenoidosa Chick). The multiphasic P680(+) reduction kinetics by YZ in PS II core complexes with high oxygen evolution capacity was seen in both algae and leaves. Model simulation to fit SFITFY curves for dark adapted species used here gives the rate constants to verify nanosecond kinetic stages of P680(+) reduction by YZ in the redox state S1 of the water oxidizing complex (WOC) shown in Kühn et al. (2004). Then a sequence of relaxation steps in the redox state S1, outlined by Renger (2012), occurs in both algae and leaves as a similar non-adiabatic ET reactions. Coupled PT is discussed briefly to understand a rearrangement of hydrogen bond protons in the protein matrix of the WOC (Umena et al., 2011). On the other hand, present studies showed a slower reoxidation of reduced QA by QB in algal cells as compared with that in a leaf that might be regarded as a consequence of differences of spatial domains at the QB-site in leaves compared to algae. Our comparative study helped to correlate theory with experimental data for molecular photosynthetic mechanisms in thylakoid membranes.
authors.affiliation: Department of Biophysics, Biology Faculty, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia. Electronic address: natalmurav@yandex.ru.
authors.forename: N E
authors.initials: NE
authors.lastname: Belyaeva
authors.affiliation: Technical University Berlin, Institute of Chemistry, Max-Volmer-Laboratory of Biophysical Chemistry, Straβe des 17. Juni 135, D-10623 Berlin, Germany.
authors.forename: F-J
authors.initials: FJ
authors.lastname: Schmitt
authors.affiliation: Department of Biophysics, Biology Faculty, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia.
authors.forename: V Z
authors.initials: VZ
authors.lastname: Paschenko
authors.affiliation: Department of Biophysics, Biology Faculty, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia.
authors.forename: G Yu
authors.initials: GY
authors.lastname: Riznichenko
authors.affiliation: Department of Biophysics, Biology Faculty, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia.
authors.forename: A B
authors.initials: AB
authors.lastname: Rubin
authors.affiliation: Department of Biophysics, Biology Faculty, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia; Technical University Berlin, Institute of Chemistry, Max-Volmer-Laboratory of Biophysical Chemistry, Straβe des 17. Juni 135, D-10623 Berlin, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Renger
mesh_terms: D002708:Chlorella / Q000737:chemistry / Q000378:metabolism*; D002734:Chlorophyll / Q000378:metabolism; D004579:Electron Transport; D005453:Fluorescence; D007834:Lasers; D008027:Light; D008954:Models, Biological; D010084:Oxidation-Reduction; D010788:Photosynthesis; D045332:Photosystem II Protein Complex / Q000378:metabolism*; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D011522:Protons; D013050:Spectrometry, Fluorescence; D018724:Spinacia oleracea / Q000737:chemistry / Q000378:metabolism*; D014867:Water / Q000737:chemistry
chemical_list: D045332:Photosystem II Protein Complex; D011522:Protons; D014867:Water; D002734:Chlorophyll
keywords: Electron transfer; Fluorescence yield; Model simulation; Photosystem II; Proton transfer; Single turnover flash; Water oxidizing complex