title: Atractylodes macrocephala polysaccharides induces mitochondrial-mediated apoptosis in glioma C6 cells.
abstract: In recent years there has been an increasing interest in naturally occurring substances in plant origin that may be used as potential chemopreventive and chemotherapeutic agents to prevent or slow the progression of chronic illnesses, such as cancer. In this study, we aimed at examining the antitumor activity of Atractylodes macrocephala polysaccharide (AMPs) in vitro using glioma C6 cells and the underlying mechanisms were also investigated. The results demonstrated that AMPs significantly inhibited proliferation of C6 cells in a concentration dependent manner by DNA fragmentation and apoptosis induction. Besides, AMPs treatment induced the loss of mitochondrial membrane potential and caused release of cytochrome c to cytosol. Furthermore, the activation of capase-3, caspase-9 and poly(ADP-ribose) polymerase (PARP) cleavage occurred following AMPs treatment in C6 cells. These results suggested that the induction of apoptosis via the mitochondrial pathway was involved in the anti-proliferative activity of AMPs against glioma C6 cells.
authors.affiliation: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China; The Institute of Skull Base Surgery and Neurooncology at Hunan, Changsha 410008, Hunan, China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China; The Institute of Skull Base Surgery and Neurooncology at Hunan, Changsha 410008, Hunan, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China; The Institute of Skull Base Surgery and Neurooncology at Hunan, Changsha 410008, Hunan, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China; The Institute of Skull Base Surgery and Neurooncology at Hunan, Changsha 410008, Hunan, China.
authors.forename: Ningrong
authors.initials: N
authors.lastname: Ye
authors.affiliation: The Institute of Skull Base Surgery and Neurooncology at Hunan, Changsha 410008, Hunan, China; Department of Neurosurgery, First Affiliated Hospital of Jishou University, Jishou 416000, Hunan, China. Electronic address: chunhaihuang@yeah.net.
authors.forename: Chunhai
authors.initials: C
authors.lastname: Huang
authors.affiliation: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China; The Institute of Skull Base Surgery and Neurooncology at Hunan, Changsha 410008, Hunan, China. Electronic address: xianruiyuan@sohu.com.
authors.forename: Xianrui
authors.initials: X
authors.lastname: Yuan
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D031174:Atractylodes / Q000737:chemistry*; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D045304:Cytochromes c / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D005910:Glioma / Q000188:drug therapy* / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats
chemical_list: D011134:Polysaccharides; D045304:Cytochromes c; D011065:Poly(ADP-ribose) Polymerases; D053148:Caspase 3; D053453:Caspase 9
keywords: Atractylodes macrocephala; Glioma; Polysaccharides