title: RecTE(Psy)-mediated recombineering in Pseudomonas syringae.
abstract: A recently developed Pseudomonas syringae recombineering system simplifies the procedure for installing specific mutations at a chosen genomic locus. The procedure involves transforming P. syringae cells expressing recombineering functions with a PCR product that contains desired changes flanked by sequences homologous to a target location. Cells transformed with the substrate undergo homologous recombination between the genomic DNA and the recombineering substrate. The recombinants are found by selection for traits carried by the recombineering substrate, usually antibiotic resistance.
authors.affiliation: Plant-Microbe Interactions Research Unit, Department of Agriculture, Agricultural Research Service, Ithaca, NY, USA.
authors.forename: Bryan
authors.initials: B
authors.lastname: Swingle
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D005818:Genetic Engineering; D059765:Homologous Recombination; D016254:Mutagenesis, Insertional; D044224:Pseudomonas syringae / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins