title: Distinctive histopathology and modulation of cytokine production during oral and intraperitoneal Trypanosoma cruzi Y strain infection.
abstract: Acute Chagas disease outbreaks are related to the consumption of food or drink contaminated by triatomine feces, thus making oral infection an important route of transmission. Both vector-borne and oral infections trigger important cardiac manifestations in the host that are related to a dysregulated immune response. The aims of this work were to evaluate possible alterations of lymphocyte CD4+/CD8+ sub-populations, Th1 and Th2 cytokines, nitrite concentrations and cardiac histopathology. One group of male Wistar rats was intraperitoneally infected (I.P.) with 1×105 metacyclic trypomastigotes of the T. cruzi Y strain, and another group of Wistar rats was orally infected (O.I.) with 8×105 metacyclic trypomastigotes of the same strain. The intraperitoneal infection triggered statistically enhanced parasite and peritoneal macrophage numbers, increased concentrations of NO and IL-12 and elevated cardiac inflammatory foci when compared with the oral infection. However, proliferation of CD4+ and CD8+ T cells were not statistically different for oral and intraperitoneal routes.
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto FCFRP-USP, Universidade de São Paulo, Avenida do Café, s/n, 14040-903, Ribeirão Preto, SP, Brazil.
authors.forename: Christian C
authors.initials: CC
authors.lastname: Kuehn
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto FCFRP-USP, Universidade de São Paulo, Avenida do Café, s/n, 14040-903, Ribeirão Preto, SP, Brazil.
authors.forename: Luiz Gustavo R
authors.initials: LG
authors.lastname: Oliveira
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto FCFRP-USP, Universidade de São Paulo, Avenida do Café, s/n, 14040-903, Ribeirão Preto, SP, Brazil.
authors.forename: Mariza Abreu
authors.initials: MA
authors.lastname: Miranda
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto FCFRP-USP, Universidade de São Paulo, Avenida do Café, s/n, 14040-903, Ribeirão Preto, SP, Brazil.
authors.forename: José Clóvis
authors.initials: JC
authors.lastname: Prado
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000469:parasitology*; D016207:Cytokines / Q000378:metabolism*; D005786:Gene Expression Regulation; D008297:Male; D018512:Parasitemia; D051381:Rats; D017208:Rats, Wistar; D016176:T-Lymphocyte Subsets; D013997:Time Factors; D014349:Trypanosoma cruzi / Q000145:classification*
chemical_list: D016207:Cytokines