title: The expression of IL6 and 21 in crossbred calves upregulated by inactivated trivalent FMD vaccine.
abstract: Foot and mouth disease (FMD) is an economically important disease and a whole-virus inactivated trivalent virus vaccine is the mainstay for controlling the disease in India. The protective humoral immune response to FMD vaccination is a complex, but, tightly regulated process mediated by the interplay of interleukins (IL). Based on the specific role of IL6 and 21 in adaptive immune response, we hypothesized that inactivated trivalent FMD vaccine would stimulate IL6 and 21 expression in the circulating lymphocytes. The expressions of IL6 and 21 were assayed on 0, 28, 60, 90, and 120 d post-vaccination (DPV) by quantitative PCR (qPCR) with simultaneous assessment of FMDV antibody titer by liquid phase blocking ELISA. The results revealed that the peak expression of IL6 and 21 was on DPV 28 which correlated well with the FMDV antibody titer and plummeted to the prevaccination titer level by 60 DPV. As IL21 is the final effector of antibody production as compared to IL6, we investigated the expression of IL21 in calves that had protective titer (>1.8) with the unprotected group (<1.8). Expression of IL21 on 28 DPV was numerically higher in the protected than that of the unprotected group of calves.
authors.affiliation: a Central Sheep & Wool Research Institute , Avikanagar , Rajasthan , India.
authors.forename: G R
authors.initials: GR
authors.lastname: Gowane
authors.forename: A K
authors.initials: AK
authors.lastname: Sharma
authors.forename: M
authors.initials: M
authors.lastname: Sankar
authors.forename: K
authors.initials: K
authors.lastname: Narayanan
authors.forename: Punam
authors.initials: P
authors.lastname: Bisht
authors.forename: S
authors.initials: S
authors.lastname: Subramaniam
authors.forename: B
authors.initials: B
authors.lastname: Pattnaik
mesh_terms: D000375:Aging / Q000276:immunology; D000818:Animals; D002417:Cattle / Q000097:blood / Q000235:genetics / Q000276:immunology*; D002418:Cattle Diseases / Q000276:immunology* / Q000517:prevention & control; D005260:Female; D005536:Foot-and-Mouth Disease / Q000276:immunology* / Q000517:prevention & control; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology; D006824:Hybridization, Genetic / Q000187:drug effects / Q000276:immunology; D015850:Interleukin-6 / Q000097:blood / Q000276:immunology*; D007378:Interleukins / Q000097:blood / Q000276:immunology*; D008297:Male; D015854:Up-Regulation / Q000187:drug effects / Q000276:immunology; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000494:pharmacology; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D000098546:Interleukin-21
chemical_list: D015850:Interleukin-6; D007378:Interleukins; D015164:Vaccines, Inactivated; D014765:Viral Vaccines; D000098546:Interleukin-21