title: Baseline dental plaque activity, mutans streptococci culture, and future caries experience in children.
abstract: OBJECTIVE
  The purpose of this study was to evaluate a chairside caries risk assessment protocol utilizing a caries prediction instrument, adenosine triphosphate (ATP) activity in dental plaque, mutans streptococci (MS) culture, and routine dental examination in five- to 10-year-old children at two regional Australian schools with high caries experience.
  
  
  METHODS
  Clinical indicators for future caries were assessed at baseline examination using a standardized prediction instrument. Plaque ATP activity was measured directly in relative light units (RLU) using a bioluminescence meter, and MS culture data were recorded. Each child's dentition was examined clinically and radiographically, and caries experience was recorded using enamel white spot lesions and decayed, missing, and filled surfaces for primary and permanent teeth indices. Univariate one-way analysis of variance between selected clinical indicators, ATP activity, MS count at baseline, and future new caries activity was performed, and a generalized linear model for prediction of new caries activity at 24 months was constructed.
  
  
  RESULTS
  Future new caries activity was significantly associated with the presence of visible cavitations, reduced saliva flow, and orthodontic appliances at baseline (R(2)=0.2, P<.001).
  
  
  CONCLUSIONS
  Baseline plaque adenosine triphosphate activity and mutans streptococci counts were not significantly associated with caries activity at 24 months.
authors.affiliation: Royal Children's Hospital, Melbourne, Australia. kerrod.hallett@rch.org.au.
authors.forename: Kerrod B
authors.initials: KB
authors.lastname: Hallett
authors.affiliation: Queensland Institute of Medical Research, Brisbane, in Australia.
authors.forename: Peter K
authors.initials: PK
authors.lastname: O'Rourke
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D058491:Bacterial Load; D002648:Child; D002675:Child, Preschool; D004245:DMF Index; D003731:Dental Caries / Q000175:diagnosis; D003732:Dental Caries Activity Tests; D003733:Dental Caries Susceptibility / Q000502:physiology*; D003773:Dental Plaque / Q000737:chemistry* / Q000382:microbiology; D005500:Follow-Up Studies; D005544:Forecasting; D006801:Humans; D016014:Linear Models; D049408:Luminescent Agents; D008163:Luminescent Measurements / Q000379:methods; D009967:Orthodontic Appliances; D010919:Placebos; D011446:Prospective Studies; D018570:Risk Assessment; D012463:Saliva / Q000378:metabolism; D012636:Secretory Rate / Q000502:physiology; D012923:Social Class; D013295:Streptococcus mutans / Q000302:isolation & purification*; D014094:Tooth, Deciduous / Q000473:pathology; D014504:Urban Health
chemical_list: D049408:Luminescent Agents; D010919:Placebos; D000255:Adenosine Triphosphate