title: Cell surface proteomics analysis indicates a neural lineage bias of rat bone marrow mesenchymal stromal cells.
abstract: Mesenchymal stromal cells (MSCs) are one of the most intensively studied stem cell types with application aims. However, the molecular characterisation and the relationship between the molecular characterisation and functional properties of MSCs are largely unknown. In this study, we purified the surface proteins from rat bone marrow MSCs (rBMMSCs) and characterised their surface proteome by LC-MS/MS. Moreover, we comparatively analysed the data from this study with the surface proteomics data of mouse and human embryonic stem (ES) cells and human mesenchymal stromal cells (hMSCs). The data showed that, in contrast to ES cells and human mesenchymal stromal cells, rBMMSCs possessed a surface proteomics pattern biased to neural and neural-endocrine lineages, indicating a neural/neural crest bias, and suggested a neural differentiation tendency of these cells. The different surface proteomics pattern between rBMMSCs and hMSCs also suggested that MSCs of different origin might possess a different lineage bias.
authors.affiliation: The Institute of Genetics, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Guiyun
authors.initials: G
authors.lastname: Zhao
authors.affiliation: The Institute of Genetics, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Huijiao
authors.initials: H
authors.lastname: Ji
authors.affiliation: The Institute of Genetics, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Shihao
authors.identifier: 0000-0001-9445-5951
authors.initials: S
authors.lastname: Wang
authors.affiliation: The Institute of Genetics, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Bin
authors.identifier: 0000-0001-9543-5450
authors.initials: B
authors.lastname: Gu
authors.affiliation: The Institute of Genetics, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Song
authors.affiliation: The Institute of Genetics, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Jiarong
authors.identifier: 0000-0002-0953-2455
authors.initials: J
authors.lastname: Zhang
authors.affiliation: The Institute of Genetics, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Yukan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Aquatic Resources and Utilization, Ministry of Education, College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai, 201306, China.
authors.forename: Liangbiao
authors.initials: L
authors.lastname: Chen
authors.affiliation: The Institute of Genetics, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism; D002454:Cell Differentiation; D019070:Cell Lineage; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis*; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D010012:Osteogenesis / Q000235:genetics; D040901:Proteomics; D051381:Rats
chemical_list: D008565:Membrane Proteins