title: Regulation of recombinant Trichinella spiralis 53-kDa protein (rTsP53) on alternatively activated macrophages via STAT6 but not IL-4Rα in vitro.
abstract: Classically activated macrophages (M1) or alternatively activated macrophages (M2) have different functions during helminth infections including Trichinella spiralis (T. spiralis). The excretory/secretory antigens (ESA) of T. spiralis can inhibit macrophage pro-inflammatory cytokines production. However, the specific molecules of ESA that regulate macrophages have not been identified. We previously reported that recombinant T. spiralis derived molecule 53-kDa protein (rTsP53) had protected mice from colitis. Furthermore, in the present study in vitro, we investigated rTsP53 showed anti-inflammatory function by inducing peritoneal macrophages to M2 with expressing M2 molecules of mannose receptor (MR), a novel mammalian lectin (Ym1), arginase-1 (Arg1), and interleukin (IL)-10. Next, we found the effect of rTsP53 on M2 independently of IL-4Rα. But rTsP53 can act dependently on signal transducers and activators of transcription 6 (STAT6). These results further imply that rTsP53 has potential as prospective immuno-therapeutics for inflammatory disorders.
authors.affiliation: Department of Intensive Care Unit, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China. Electronic address: du_linlin2007@163.com.
authors.forename: Linlin
authors.initials: L
authors.lastname: Du
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wei
authors.affiliation: Department of Intensive Care Unit, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
authors.forename: Leiqing
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Intensive Care Unit, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Shan
authors.affiliation: Department of Intensive Care Unit, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Intensive Care Unit, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
authors.forename: Yesong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Intensive Care Unit, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
authors.forename: Gensheng
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D001119:Arginase / Q000235:genetics / Q000276:immunology; D005260:Female; D005786:Gene Expression Regulation; D015801:Helminth Proteins / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D006790:Host-Parasite Interactions; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology; D037102:Lectins / Q000235:genetics / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000276:immunology / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D061251:Primary Cell Culture; D011956:Receptors, Cell Surface / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D050800:STAT6 Transcription Factor / Q000235:genetics* / Q000276:immunology; D015398:Signal Transduction; D017160:Trichinella spiralis / Q000737:chemistry* / Q000235:genetics; D001619:beta-N-Acetylhexosaminidases / Q000235:genetics / Q000276:immunology; D053662:Interleukin-4 Receptor alpha Subunit
chemical_list: D000947:Antigens, Helminth; D001119:Arginase; D015801:Helminth Proteins; D016753:Interleukin-10; D037102:Lectins; D008070:Lipopolysaccharides; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D050800:STAT6 Transcription Factor; D001619:beta-N-Acetylhexosaminidases; C556062:IL10 protein, mouse; C504002:Il4ra protein, mouse; D053662:Interleukin-4 Receptor alpha Subunit; C494100:Stat6 protein, mouse; C429541:Chil3 protein, mouse; C544448:Arg1 protein, mouse
keywords: 53-kDa protein; Excretory/secretory antigen; Macrophage phenotype; Trichinella spiralis