title: An effective method for extracting total RNA from Dioscorea opposita Thunb.
abstract: Dioscorea opposita Thunb., included in the genus Dioscorea of the family Dioscoreaceae, is an important herb with great edible and medicinal value. In this study, the total RNA from leaves of Lichuan Dioscorea opposita Thunb. was isolated by an improved Trizol method. The results showed that the RNA extracted by the improved Trizol method had good integrity, and the RNA could be used for down-stream molecular biology operations including reverse transcription-polymerase chain reaction.
authors.affiliation: Library, China West Normal University, Nanchong City, China.
authors.forename: C M
authors.initials: CM
authors.lastname: Liao
authors.affiliation: College of Life Science, China West Normal University, Nanchong City, China.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Life Science, China West Normal University, Nanchong City, China.
authors.forename: X H
authors.initials: XH
authors.lastname: Liu
authors.affiliation: College of Life Science, China West Normal University, Nanchong City, China liuxiaohong0817@aliyun.com.
authors.forename: Y S
authors.initials: YS
authors.lastname: Zhang
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D027742:Dioscorea / Q000737:chemistry*; D012313:RNA / Q000302:isolation & purification*; D018749:RNA, Plant / Q000302:isolation & purification*
chemical_list: D018749:RNA, Plant; D012313:RNA