title: Metabolomic approach for discrimination of processed ginseng genus (Panax ginseng and Panax quinquefolius) using UPLC-QTOF MS.
abstract: Discriminating between two herbal medicines (Panax ginseng and Panax quinquefolius), with similar chemical and physical properties but different therapeutic effects, is a very serious and difficult problem. Differentiation between two processed ginseng genera is even more difficult because the characteristics of their appearance are very similar. An ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF MS)-based metabolomic technique was applied for the metabolite profiling of 40 processed P. ginseng and processed P. quinquefolius. Currently known biomarkers such as ginsenoside Rf and F11 have been used for the analysis using the UPLC-photodiode array detector. However, this method was not able to fully discriminate between the two processed ginseng genera. Thus, an optimized UPLC-QTOF-based metabolic profiling method was adapted for the analysis and evaluation of two processed ginseng genera. As a result, all known biomarkers were identified by the proposed metabolomics, and additional potential biomarkers were extracted from the huge amounts of global analysis data. Therefore, it is expected that such metabolomics techniques would be widely applied to the ginseng research field.
authors.affiliation: Korea Ginseng Corporation Research Institute, Korea Ginseng Corporation, Daejeon, Korea.
authors.forename: Hee-Won
authors.initials: HW
authors.lastname: Park
authors.affiliation: Korea Ginseng Corporation Research Institute, Korea Ginseng Corporation, Daejeon, Korea.
authors.forename: Gyo
authors.initials: G
authors.lastname: In
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul, Korea.
authors.forename: Jeong-Han
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Korea Ginseng Corporation Research Institute, Korea Ginseng Corporation, Daejeon, Korea.
authors.forename: Byung-Goo
authors.initials: BG
authors.lastname: Cho
authors.affiliation: Korea Ginseng Corporation Research Institute, Korea Ginseng Corporation, Daejeon, Korea.
authors.forename: Gyeong-Ho
authors.initials: GH
authors.lastname: Han
authors.affiliation: Korea Ginseng Corporation Research Institute, Korea Ginseng Corporation, Daejeon, Korea.
authors.forename: Il-Moo
authors.initials: IM
authors.lastname: Chang
keywords: Panax ginseng; Panax quinquefolius; UPLC-QTOF MS; discrimination; metabolomics