title: Effects of early and late pneumothorax drainage on the development of pulmonary oedema.
abstract: We analyzed the effects of pneumothorax duration and early or late drainage on lung histology and biological markers associated with inflammation, alveolar fluid clearance, and pulmonary oedema formation. Pneumothorax was induced by injecting air into the thorax of anaesthetized rats, which were randomized according to duration of pneumothorax [5 (PTX5) or 30 (PTX30)min] and further divided to be drained (D) or not (ND). ND rats were euthanized at 5 and 30min. In D groups, pneumothorax was drained and rats breathed spontaneously for 30min. PTX30-ND, compared to PTX5-ND, showed higher alveolar collapse and oedema, type III procollagen, caspase-3, epithelial sodium channel-α, and aquaporin (AQP)-1 mRNA expression, and epithelial and endothelial damage, with reduced cystic fibrosis transmembrane conductance regulator (CFTR) and AQP-3 expression. PTX5-D, compared to PTX30-D, showed less alveolar hyperinflation, oedema, and alveolar-capillary damage, with reduced interleukin-6, caspase-3, AQP-5, and Na,K-ATPase-α and -β expression, and increased CFTR expression. In conclusion, longer duration pneumothorax exacerbated lung damage, oedema, and inflammation.
authors.affiliation: Laboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Avenida Carlos Chagas Filho, s/n, Bloco G-014, Ilha do Fundão, 21941-902 Rio de Janeiro, Brazil; Department of Surgery, Faculty of Medicine, Federal University of Rio de Janeiro, Avenida Professor Rodolpho Paulo Rocco, 225, Ilha do Fundão, 21941-913 Rio de Janeiro, Brazil.
authors.forename: Alessandra S N T
authors.initials: AS
authors.lastname: Elias
authors.affiliation: Laboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Avenida Carlos Chagas Filho, s/n, Bloco G-014, Ilha do Fundão, 21941-902 Rio de Janeiro, Brazil.
authors.forename: Gisele P
authors.initials: GP
authors.lastname: Oliveira
authors.affiliation: Laboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Avenida Carlos Chagas Filho, s/n, Bloco G-014, Ilha do Fundão, 21941-902 Rio de Janeiro, Brazil; Laboratory of Cellular and Molecular Physiology, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Avenida Carlos Chagas Filho, s/n, Bloco G2-048, Ilha do Fundão, 21941-902 Rio de Janeiro, Brazil.
authors.forename: Débora S
authors.initials: DS
authors.lastname: Ornellas
authors.affiliation: Laboratory of Cellular and Molecular Physiology, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Avenida Carlos Chagas Filho, s/n, Bloco G2-048, Ilha do Fundão, 21941-902 Rio de Janeiro, Brazil.
authors.forename: Marcelo M
authors.initials: MM
authors.lastname: Morales
authors.affiliation: Department of Pathology, Faculty of Medicine, University of São Paulo, Avenida Doutor Arnaldo, 455, 01246-903 São Paulo, Brazil.
authors.forename: Vera L
authors.initials: VL
authors.lastname: Capelozzi
authors.affiliation: Department of Surgery, Faculty of Medicine, Federal University of Rio de Janeiro, Avenida Professor Rodolpho Paulo Rocco, 225, Ilha do Fundão, 21941-913 Rio de Janeiro, Brazil.
authors.forename: Rui
authors.initials: R
authors.lastname: Haddad
authors.affiliation: IRCCS AOU San Martino-IST, Department of Surgical Sciences and Integrated Diagnostics, University of Genoa, Largo Rosanna Benzi 8, 16132 Genoa, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Pelosi
authors.affiliation: Laboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Avenida Carlos Chagas Filho, s/n, Bloco G-014, Ilha do Fundão, 21941-902 Rio de Janeiro, Brazil. Electronic address: prmrocco@biof.ufrj.br.
authors.forename: Patricia R M
authors.initials: PR
authors.lastname: Rocco
authors.affiliation: Laboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Avenida Carlos Chagas Filho, s/n, Bloco G-014, Ilha do Fundão, 21941-902 Rio de Janeiro, Brazil; Rio de Janeiro Federal Institute of Education, Science and Technology, Rua Carlos Wenceslau, n° 343, Realengo, 21715-000 Rio de Janeiro, RJ, Brazil.
authors.forename: Cristiane S N B
authors.initials: CS
authors.lastname: Garcia
mesh_terms: D000818:Animals; D051398:Aquaporin 1 / Q000378:metabolism; D051400:Aquaporin 3 / Q000378:metabolism; D051402:Aquaporin 5 / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D024061:Collagen Type III / Q000378:metabolism; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000378:metabolism; D004322:Drainage; D004727:Endothelium / Q000473:pathology; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D011030:Pneumothorax / Q000150:complications / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D011650:Pulmonary Alveoli / Q000473:pathology; D011654:Pulmonary Edema / Q000209:etiology* / Q000276:immunology / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D020545:Respiratory Mucosa / Q000473:pathology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013997:Time Factors
chemical_list: C496022:Aqp1 protein, rat; C496029:Aqp3 protein, rat; C496040:Aqp5 protein, rat; D051402:Aquaporin 5; C579654:CFTR protein, rat; D024061:Collagen Type III; D015850:Interleukin-6; D012333:RNA, Messenger; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D051398:Aquaporin 1; D051400:Aquaporin 3; C505410:Casp3 protein, rat; D053148:Caspase 3; D000254:Sodium-Potassium-Exchanging ATPase
keywords: Alveolar cell biology; Alveolar fluid clearance; Alveolar-capillary permeability; Inflammation; Lung oedema