title: Bi-pseudoenzyme synergetic catalysis to generate a coreactant of peroxydisulfate for an ultrasensitive electrochemiluminescence-based cholesterol biosensor.
abstract: A novel electrochemiluminescence (ECL) enzyme biosensor for the ultrasensitive detection of cholesterol was designed based on a bi-pseudoenzymatic reaction to generate a coreactant of peroxydisulfate for signal ampliﬁcation. In this work, hemin-functionalized graphene (hemin-GR) was synthesized and used to immobilize cholesterol oxidase (COx) to construct an ECL biosensor for cholesterol. When cholesterol was added to the detection solution, COx catalyzed the oxidation of cholesterol to generate H2O2, which could be further catalyzed by hemin to produce O2 as the coreactant in the peroxydisulfate system for signal ampliﬁcation. The linear range for cholesterol detection was 3.3-1,500 nM, with a lower detection limit of 1.0 nM (signal to noise ratio=3). Therefore, the detection limit and sensitivity of the biosensor were improved. This novel strategy offers advantages of simplicity, improved sensitivity, good selectivity, and repeatability, and therefore, holds promise for use in sensitive bioassays for clinical determination of cholesterol levels.
authors.affiliation: Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Wanting
authors.initials: W
authors.lastname: Wang
authors.affiliation: Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China. Electronic address: cshong@swu.edu.cn.
authors.forename: Shihong
authors.initials: S
authors.lastname: Chen
authors.affiliation: Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China. Electronic address: yuanruo@swu.edu.cn.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Ruo
authors.affiliation: Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhong
authors.affiliation: Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Wu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D001951:Brevibacterium / Q000201:enzymology; D002784:Cholesterol / Q000097:blood*; D004800:Enzymes, Immobilized / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006427:Hemin / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods*; D010545:Peroxides / Q000737:chemistry; D013431:Sulfates / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D010545:Peroxides; D013431:Sulfates; D006427:Hemin; D006108:Graphite; D002784:Cholesterol; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase
keywords: Biosensor; Cholesterol; Electrochemiluminescence; Graphene; Hemin; Peroxydisulfate