title: Determination of hydroxysafflor yellow A in biological fluids of patients with traumatic brain injury by UPLC-ESI-MS/MS after injection of Xuebijing.
abstract: A simple, novel, specific, rapid and reproducible ultra-performance liquid chromatography electrospray ionization tandem mass spectrometry method has been developed and validated for the determination of hydroxysafflor yellow A (HSYA) in biological fluids (plasma, urine and cerebrospinal fluid) of patients with traumatic brain injury after intravenous injection of Xuebijing (XBJ). Liquid-liquid extraction was performed, and separation was carried out on an Acquity UPLC™ BEH C18 column, with gradient elution using a mobile phase composed of methanol and 0.1% formic acid at a flow rate of 0.3 mL/min. A triple quadrupole tandem mass spectrometer with electrospray ionization was used for the detection of HSYA. The mass transition followed was m/z 611.0 → 491. The retention time was less than 3.0 min. The calibration curve was linear in the concentration range from 2 to 6125 ng/mL for cerebrospinal fluid, plasma and urine. The intra- and inter-day precisions were <10%, and the relative standard deviation of recovery was <15% for HSYA in biological matrices. The method was successfully applied for the first time to quantify HSYA in the biological fluids (especially in cerebrospinal fluid) of patients with traumatic brain injury following intravenous administration of XBJ.
authors.affiliation: Department of Neurosurgery, Xiangya Hospital, Central South University, 410008, Changsha, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Guo
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Xi
authors.initials: X
authors.lastname: Huang
authors.forename: Huiying
authors.initials: H
authors.lastname: Lv
authors.forename: Rong
authors.initials: R
authors.lastname: Fan
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Pingping
authors.initials: P
authors.lastname: Gan
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Kuipo
authors.initials: K
authors.lastname: Yan
authors.forename: Zian
authors.initials: Z
authors.lastname: Xia
authors.forename: Jinfang
authors.initials: J
authors.lastname: Liu
mesh_terms: D001930:Brain Injuries / Q000188:drug therapy* / Q000378:metabolism*; D002599:Chalcone / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics / Q000627:therapeutic use*; D006801:Humans; D007275:Injections, Intravenous; D016014:Linear Models; D011809:Quinones / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D004365:Drugs, Chinese Herbal; D011809:Quinones; C549376:Xuebijing; C085278:hydroxysafflor yellow A; D002599:Chalcone
keywords: UPLC-MS/MS; Xuebijing injection; biological fluids; hydroxysafflor yellow A; traumatic brain injury