title: [Non-invasive fetal RHD genotyping: Validation of the method with 200 patients].
abstract: BACKGROUND
  Non-invasive fetal RHD genotyping is an important tool to assess the risk of fetuse's hemolytic disease of anti-D allo-immunized pregnant woman by non-invasive method. A method of genotyping has been developed in the laboratory of Lyon-GHE according to Minon's team (J Gynecol Obstet Biol Reprod 2005): exon 4, 5, and 10 are amplified by real time PCR. At first, genotyping results of 200 pregnant women have been compared with RH1 phenotype at birth. The most important parameters of validation have been tested: the sensibility and the specificity; the negative predictive value; the correlation study permitted to define criteria of biological interpretation. The validation of this method permitted to determine critical points and the limits of the method due to the minor amount of fetal DNA in the maternal plasma and existence of many variant forms of the RHD gene.
  
  
  CONCLUSIONS
  We worked too in the perspective to the accreditation for our genetic laboratory.
authors.affiliation: EFS Rhône-Alpes, site de Lyon-GHE, 28, rue Doyen-Lepine, 69677 Bron cedex, France. Electronic address: emmanuelle.guinchard@efs.sante.fr.
authors.forename: E
authors.initials: E
authors.lastname: Guinchard
authors.affiliation: EFS Rhône-Alpes, site de Lyon-GHE, 28, rue Doyen-Lepine, 69677 Bron cedex, France.
authors.forename: P
authors.initials: P
authors.lastname: Bricca
authors.affiliation: EFS Rhône-Alpes, site de Lyon-GHE, 28, rue Doyen-Lepine, 69677 Bron cedex, France.
authors.forename: S
authors.initials: S
authors.lastname: Monnier
authors.affiliation: EFS Rhône-Alpes, site de Lyon-Gerland, 1, rue du Vercors, 69007 Lyon, France.
authors.forename: D
authors.initials: D
authors.lastname: Rigal
mesh_terms: D005260:Female; D005333:Fetus / Q000276:immunology*; D005838:Genotype; D006801:Humans; D011247:Pregnancy / Q000097:blood*; D011296:Prenatal Diagnosis / Q000379:methods*; D012204:Rh-Hr Blood-Group System / Q000097:blood* / Q000235:genetics*
chemical_list: D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen
keywords: Accreditation; Accréditation; Allo-immunisation; Allo-immunization; Diagnostic prénatal; Génotypage par méthode non invasive; Non-invasive genotyping; Phénotype RH1; Prenatal diagnosis; RH1 phenotype; Validation de méthode; Validation of the method
vernacular_title: RHD fœtal sur plasma maternel : validation de méthode sur 200 patientes.