title: Coxiella burnetii serology assays in goat abortion storm.
abstract: Many commercial antibody detection enzyme-linked immunosorbent assay (ELISA) kits for Q fever utilize the Nine Mile (Montana tick) strain of Coxiella burnetii as antigen. An ELISA kit manufactured in France employs ovine placenta-sourced antigen and has been used in Europe. Sera from goats experiencing a Q fever abortion storm in the United States were used to compare the sensitivity and specificity of these 2 ELISA formats and the Q fever complement fixation test (CFT). Latent class estimates of sensitivity ranged from 97% to 100% with a specificity of 95-100% for the 2 ELISA kits. Estimates for sensitivity and specificity of the CFT were 89% and 82%, respectively. There was not a significant increase in ELISA sensitivity observed with the ovine-sourced antigen kit in this study. Real-time polymerase chain reactions performed on a portion of the sera found that 15 out of 20 sera were congruent across 4 tests for positive and negative sera.
authors.affiliation: 1Michelle P. Emery, National Veterinary Services Laboratories, Diagnostic Virology Laboratory, 1920 Dayton Avenue, Ames, IA 50010. michelle.p.emery@aphis.usda.gov.
authors.forename: Michelle P
authors.initials: MP
authors.lastname: Emery
authors.forename: Eileen N
authors.initials: EN
authors.lastname: Ostlund
authors.forename: Mohamed
authors.initials: M
authors.lastname: Ait Ichou
authors.forename: Jeff D
authors.initials: JD
authors.lastname: Ballin
authors.forename: David
authors.initials: D
authors.lastname: McFarling
authors.forename: Luanne
authors.initials: L
authors.lastname: McGonigle
mesh_terms: D000034:Abortion, Veterinary / Q000382:microbiology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D003168:Complement Fixation Tests / Q000662:veterinary; D016997:Coxiella burnetii / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004196:Disease Outbreaks / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D015511:Goat Diseases / Q000097:blood / Q000382:microbiology*; D006041:Goats; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011247:Pregnancy; D011778:Q Fever / Q000097:blood / Q000382:microbiology / Q000662:veterinary*; D012680:Sensitivity and Specificity; D014481:United States
chemical_list: D000907:Antibodies, Bacterial; D004269:DNA, Bacterial
keywords: Abortion storm; Coxiella burnetii; Nine Mile; ovine enzyme-linked immunosorbent assay; polymerase chain reaction