title: Stress in Phycomyces blakesleeanus by glucose starvation and acetate growth: response of the antioxidant system and reserve carbohydrates.
abstract: The objective of this study was to analyze the response of Phycomyces blakesleeanus to glucose starvation and acetate growth stress. At the onset of the exponential growth phase, the fungus shows a high tolerance to both stresses, being higher for the glucose starvation. In both stresses we have found higher activities of catalase and glutathione peroxidase, and a decrease of the pools of D-erythroascorbate (D-erythroascorbate+D-erythroascorbate monoglucoside) and glutathione (GSH+GSSG), while the intracellular GSH/GSSG redox balance becomes more reducing. Gallic acid was not detected under both stresses. Glycogen breakdown and the high levels of trehalose seem to be part of the stress response. Both stress, under the conditions of this study, seem to lead to a qualitatively similar response in P. blakesleeanus, with regard to the behavior of antioxidant system, the content of secondary metabolites and the role of the reserve carbohydrates.
authors.affiliation: Department of Molecular Biology, University of León, 24071 León, Spain. Electronic address: javier.rua@unileon.es.
authors.forename: Javier
authors.initials: J
authors.lastname: Rúa
authors.affiliation: Department of Molecular Biology, University of León, 24071 León, Spain. Electronic address: ccasc@unileon.es.
authors.forename: Cristina
authors.initials: C
authors.lastname: de Castro
authors.affiliation: Department of Molecular Biology, University of León, 24071 León, Spain. Electronic address: dolores.arriaga@unileon.es.
authors.forename: Dolores
authors.initials: D
authors.lastname: de Arriaga
authors.affiliation: Department of Food Hygiene and Food Technology, University of León, 24071 León, Spain. Electronic address: mrgara@unileon.es.
authors.forename: María Rosario
authors.initials: MR
authors.lastname: García-Armesto
authors.affiliation: Department of Molecular Biology, University of León, 24071 León, Spain. Electronic address: fbusto@unileon.es.
authors.forename: Félix
authors.initials: F
authors.lastname: Busto
authors.affiliation: Department of Molecular Biology, University of León, 24071 León, Spain. Electronic address: mpvalf@unileon.es.
authors.forename: Pilar
authors.initials: P
authors.lastname: del Valle
mesh_terms: D000085:Acetates / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D064688:Metabolic Flux Analysis; D010800:Phycomyces / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D013312:Stress, Physiological
chemical_list: D000085:Acetates; D005947:Glucose
keywords: Acetate growth stress; Antioxidant system; Glucose starvation stress; Mucoromycotina; Reserve carbohydrates