title: A global investigation of gene deletion strains that affect premature stop codon bypass in yeast, Saccharomyces cerevisiae.
abstract: Protein biosynthesis is an orderly process that requires a balance between rate and accuracy. To produce a functional product, the fidelity of this process has to be maintained from start to finish. In order to systematically identify genes that affect stop codon bypass, three expression plasmids, pUKC817, pUKC818 and pUKC819, were integrated into the yeast non-essential loss-of-function gene array (5000 strains). These plasmids contain three different premature stop codons (UAA, UGA and UAG, respectively) within the LacZ expression cassette. A fourth plasmid, pUKC815 that carries the native LacZ gene was used as a control. Transformed strains were subjected to large-scale β-galactosidase lift assay analysis to evaluate production of β-galactosidase for each gene deletion strain. In this way 84 potential candidate genes that affect stop codon bypass were identified. Three candidate genes, OLA1, BSC2, and YNL040W, were further investigated, and were found to be important for cytoplasmic protein biosynthesis.
authors.affiliation: Department of Biology and Ottawa Institute of Systems Biology, Carleton University, Ottawa, Ontario, Canada. ashkan_golshani@carleton.ca.
authors.forename: Bahram
authors.initials: B
authors.lastname: Samanfar
authors.forename: Le Hoa
authors.initials: le H
authors.lastname: Tan
authors.forename: Kristina
authors.initials: K
authors.lastname: Shostak
authors.forename: Firoozeh
authors.initials: F
authors.lastname: Chalabian
authors.forename: Zongbin
authors.initials: Z
authors.lastname: Wu
authors.forename: Md
authors.initials: M
authors.lastname: Alamgir
authors.forename: Noor
authors.initials: N
authors.lastname: Sunba
authors.forename: Daniel
authors.initials: D
authors.lastname: Burnside
authors.forename: Katayoun
authors.initials: K
authors.lastname: Omidi
authors.forename: Mohsen
authors.initials: M
authors.lastname: Hooshyar
authors.forename: Imelda
authors.initials: I
authors.lastname: Galván Márquez
authors.forename: Matthew
authors.initials: M
authors.lastname: Jessulat
authors.forename: Myron L
authors.initials: ML
authors.lastname: Smith
authors.forename: Mohan
authors.initials: M
authors.lastname: Babu
authors.forename: Ali
authors.initials: A
authors.lastname: Azizi
authors.forename: Ashkan
authors.initials: A
authors.lastname: Golshani
mesh_terms: D000251:Adenosine Triphosphatases / Q000096:biosynthesis / Q000235:genetics*; D018389:Codon, Nonsense / Q000235:genetics; D017353:Gene Deletion; D007763:Lac Operon / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D014176:Protein Biosynthesis / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000235:genetics*; D064506:Solute Carrier Family 12, Member 2 / Q000096:biosynthesis / Q000235:genetics*; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D018389:Codon, Nonsense; D029701:Saccharomyces cerevisiae Proteins; D064506:Solute Carrier Family 12, Member 2; D001616:beta-Galactosidase; D000251:Adenosine Triphosphatases; C000593184:OLA1 protein, S cerevisiae