title: Circulating Trypanosoma cruzi populations differ from those found in the tissues of the same host during acute experimental infection.
abstract: We evaluated the presence and distribution of two Trypanosoma cruzi natural isolates in blood, heart, skeletal muscle, liver, and spleen tissues in the acute phase of the experimental infection (35 days postinfection) in order to determine if the populations present in blood were different to those found in the tissues of the same host. Thirty mice were infected with 50 forms of each isolate or with a combination of them. Presence and molecular characterization of the parasites in the host tissues were determined by specific PCR. Cardiac and skeletal muscle alterations were analyzed by histological studies. T. cruzi variability in the host tissues was analyzed through RFLP studies. Both isolates used consisted of a mixture of two T. cruzi lineages. Specific PCRs were positive for most of the samples from the 3 groups analyzed. Cardiac and skeletal muscle sections from the groups infected with one isolate presented mild to moderate inflammatory infiltrates; the group infected with both isolates showed severe inflammatory infiltrates and the presence of amastigote nests in both tissues. Different parasite populations were found in circulation and in the tissues from the same host. These results are important for patients with high probability of mixed infections in endemic areas and contribute to the knowledge of parasite/host interactions.
authors.affiliation: Instituto de Investigaciones en Ciencias de la Salud (INICSA), CONICET - and Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba. Santa Rosa 1085. Córdoba, Argentina. Electronic address: silvilopresti@gmail.com.
authors.forename: M Silvina
authors.initials: MS
authors.lastname: Lo Presti
authors.affiliation: Instituto de Investigaciones en Ciencias de la Salud (INICSA), CONICET - and Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba. Santa Rosa 1085. Córdoba, Argentina.
authors.forename: Blanca H
authors.initials: BH
authors.lastname: Esteves
authors.affiliation: Instituto de Investigaciones en Ciencias de la Salud (INICSA), CONICET - and Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba. Santa Rosa 1085. Córdoba, Argentina.
authors.forename: Diego
authors.initials: D
authors.lastname: Moya
authors.affiliation: Instituto de Investigaciones en Ciencias de la Salud (INICSA), CONICET - and Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba. Santa Rosa 1085. Córdoba, Argentina.
authors.forename: P Carolina
authors.initials: PC
authors.lastname: Bazán
authors.affiliation: Instituto de Investigaciones en Ciencias de la Salud (INICSA), CONICET - and Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba. Santa Rosa 1085. Córdoba, Argentina.
authors.forename: Mariana
authors.initials: M
authors.lastname: Strauss
authors.affiliation: Instituto de Investigaciones en Ciencias de la Salud (INICSA), CONICET - and Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba. Santa Rosa 1085. Córdoba, Argentina.
authors.forename: Alejandra L
authors.initials: AL
authors.lastname: Báez
authors.affiliation: Cátedra de Parasitología. Facultad de Ciencias Médicas. Universidad Nacional de Córdoba, Córdoba, Argentina.
authors.forename: Rogelio
authors.initials: R
authors.lastname: Pizzi
authors.affiliation: Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias. La Laguna, Tenerife, Spain.
authors.forename: M Antonieta
authors.initials: MA
authors.lastname: Quispe Ricalde
authors.affiliation: Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias. La Laguna, Tenerife, Spain.
authors.forename: Basilio
authors.initials: B
authors.lastname: Valladares
authors.affiliation: Instituto de Investigaciones en Ciencias de la Salud (INICSA), CONICET - and Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba. Santa Rosa 1085. Córdoba, Argentina; Cátedra de Física Biomédica. Facultad de Medicina. Universidad Nacional de La Rioja, La Rioja, Argentina.
authors.forename: H Walter
authors.initials: HW
authors.lastname: Rivarola
authors.affiliation: Instituto de Investigaciones en Ciencias de la Salud (INICSA), CONICET - and Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba. Santa Rosa 1085. Córdoba, Argentina.
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Paglini-Oliva
mesh_terms: D000818:Animals; D001769:Blood / Q000469:parasitology*; D014355:Chagas Disease / Q000469:parasitology* / Q000473:pathology; D016054:DNA, Protozoan / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D008297:Male; D051379:Mice; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D014349:Trypanosoma cruzi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D016054:DNA, Protozoan
keywords: Acute phase of Chagas disease; Trypanosoma cruzi lineages or DTUs; natural isolates; tissue distribution