title: [Determination of oleanolic acid in fufang zhenqin feining capsules by RP-HPLC].
abstract: OBJECTIVE
  To investigate the optimal technology of extraction of fufang zhenqin feining capsules and establish a method for determination its oleanolic acid.
  
  
  METHODS
  Orthogonal test was employed for selecting the optimum extraction technology by the index of the content of oleanolic acid in extraction. HPLC was performed on a C18 (250 mm x 4.6 mm, 5 microm) column using methanol-0.3% triethylamine solution (80: 20) as the mobile phase at a flow rate of 1 ml/min. The detection wavelength was set at 210 nm. The column temperature was 30 degrees C and the injection size was 25 microL.
  
  
  RESULTS
  The optimum extraction technology was as follows: the concertration of alcohol was 70%, the ratio of solid/material was 8: 1, extracting 3 times with half an hour for each time. The linear range of oleanolic acid was 59.68 - 596.8 microg/ml (r = 0.9997), the average recovery was 99.79% (RSD = 0.5%).
  
  
  CONCLUSIONS
  The considerable extraction rate of active components in the drugs is achieved by applying the selected technology, and the simple method is fit for production. The established HPLC method is simple, accurate, special and can be used for the quality control of fufang zhenqin feining capsules.
authors.affiliation: Guangzhou Chest Hospital, Guangzhou 510095, China.
authors.forename: Lu-Ping
authors.initials: LP
authors.lastname: Rong
authors.affiliation: The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai 519000, China.
authors.forename: Yi-Shen
authors.initials: YS
authors.lastname: Chen
authors.affiliation: School of Traditional Chinese Medicine, Guangdong Pharmaceutical University,Guangzhou 510006, China.
authors.forename: Hua-Wei
authors.initials: HW
authors.lastname: Liao
authors.affiliation: The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai 519000, China.
authors.forename: Zheng-Lan
authors.initials: ZL
authors.lastname: Wan
mesh_terms: D002214:Capsules; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D009828:Oleanolic Acid / Q000032:analysis*; D011786:Quality Control
chemical_list: D002214:Capsules; D009828:Oleanolic Acid