title: Antibacterial multilayer films fabricated by layer-by-layer immobilizing lysozyme and gold nanoparticles on nanofibers.
abstract: Negatively charged gold nanoparticles (GNP) and positively charged lysozyme (Lys) were alternately deposited on negatively charged cellulose mats via layer-by-layer (LBL) self-assembly technique. The fabricated multilayer films were characterized by energy-dispersive X-ray (EDX), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectra (FT-IR), and wide-angle X-ray diffraction (XRD). Morphology of the LBL film coated mats was observed by scanning electron microscopy (SEM). Thermal degradation properties were investigated by differential scanning calorimetry (DSC) and thermo-gravimetric analysis (TGA). Additionally, the result of microbial inhibition assay indicated that the composite nanofibrous mats had excellent antibacterial activity against Escherichia coli and Staphylococcus aureus, which could be used for antimicrobial packing, tissue engineering, wound dressing, etc.
authors.affiliation: College of Food Science and technology, Huazhong Agriculture University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.affiliation: College of Food Science and technology, Huazhong Agriculture University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
authors.forename: Hongbing
authors.initials: H
authors.lastname: Deng
authors.affiliation: College of Food Science and technology, Huazhong Agriculture University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Hu
authors.affiliation: College of Food Science and technology, Huazhong Agriculture University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, China. Electronic address: libinfood@mail.hzau.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects; D006046:Gold / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism; D057139:Nanofibers / Q000737:chemistry*; D010316:Particle Size; D013211:Staphylococcus aureus / Q000187:drug effects; D013329:Structure-Activity Relationship; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents; C005062:acetylcellulose; D006046:Gold; D002482:Cellulose; D009113:Muramidase
keywords: Bacterial inhibition activity; Electrospinning; Gold nanoparticles; Layer-by-layer; Lysozyme