title: Engineering protein-responsive mRNA switch in mammalian cells.
abstract: Engineering of translation provides an alternative regulatory layer for controlling transgene expression in addition to transcriptional regulation. Synthetic mRNA switches that modulate translation of a target gene of interest in response to an intracellular protein could be a key regulator to construct a genetic circuit. Insertion of a protein binding RNA sequence in the 5' UTR of mRNA would allow for the generation of a protein-responsive RNA switch. Here we describe the design principle of the switch and methods for tuning and analyzing its translational activity in mammalian cells.
authors.affiliation: Department of Reprogramming Science, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Endo
authors.forename: Hirohide
authors.initials: H
authors.lastname: Saito
mesh_terms: D020121:5' Untranslated Regions; D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D005434:Flow Cytometry / Q000379:methods; D017930:Genes, Reporter; D005818:Genetic Engineering / Q000379:methods*; D006801:Humans; D010957:Plasmids / Q000235:genetics; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D058928:Riboswitch; D014162:Transfection
chemical_list: D020121:5' Untranslated Regions; D011506:Proteins; D012333:RNA, Messenger; D058928:Riboswitch