title: Biodegradable poly(ester urethane) urea scaffolds for tissue engineering: Interaction with osteoblast-like MG-63 cells.
abstract: Porous three-dimensional scaffolds with potential for application as cancellous bone graft substitutes were prepared from aliphatic segmented poly(ester urethane) urea using the phase-inverse technique. Proton nuclear magnetic resonance, size-exclusion chromatography, electron spectroscopy for chemical analysis, secondary ion mass spectrometry, infrared spectroscopy, scanning electron microscopy, differential scanning calorimetry, computed tomography and mechanical tests were carried out, to characterize the scaffolds' physicochemical properties. Human osteosarcoma MG-63 cells were seeded into the scaffolds for 1, 2, 3 and 4weeks to evaluate their potential to support attachment, growth and proliferation of osteogenic cells. The scaffold-cell interaction was assessed by analysis of DNA content, total protein amount, alkaline phosphatase activity and WST-1 assay. The scaffolds supported cell attachment, growth and proliferation over the whole culture period of 4weeks (DNA, total protein amount). There was, however, a reduction in the WST-1 assay values at 4weeks, which might suggest a reduction in the rate of cell proliferation at this time.
authors.affiliation: Department of Orthopaedics & Traumatology, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region.
authors.forename: Joanna
authors.initials: J
authors.lastname: Podporska-Carroll
authors.affiliation: Department of Orthopaedics & Traumatology, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region.
authors.forename: Josephine W Y
authors.initials: JW
authors.lastname: Ip
authors.affiliation: PolyBiomat Consulting, Zielona Gora, Poland. Electronic address: sylwester.gogolewski@neostrada.pl.
authors.forename: Sylwester
authors.initials: S
authors.lastname: Gogolewski
mesh_terms: D001672:Biocompatible Materials; D002152:Calorimetry, Differential Scanning; D045744:Cell Line, Tumor; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology*; D056951:Photoelectron Spectroscopy; D011091:Polyesters; D013055:Spectrophotometry, Infrared; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C497893:poly(ester urethane)urea
keywords: Aliphatic poly(ester urethane) urea; MG-63 cell culture; Porous scaffolds; SIMS-ESCA analysis; WST-1 assay