title: Lipidomic profiling of snow algae by ESI-MS and silver-LC/APCI-MS.
abstract: The main analytical benefit of this study is the development of methods enabling a rapid determination of total lipids of algae by lipidomic analysis and detailed identification and quantification of a complex mixture of natural TAGs by silver-LC/APCI-MS and NARP-LC/APCI-MS. Both types of chromatography can readily identify, both qualitatively and semiquantitatively, triacylglycerols containing 16:3 and 16:4 acids in the molecule. We conclude that the genus Chloromonas is a major producer of C16 PUFAs mostly contained in TAGs. Since more detailed studies in this field have been stymied by the shortage of 16:3 and 16:4 FAs, we decided to study the alga Chloromonas as a potential biotechnological source of C16 PUFAs.
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20 Prague, Czech Republic. Electronic address: rezanka@biomed.cas.cz.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Rezanka
authors.affiliation: Charles University in Prague, Faculty of Science, Department of Ecology, Viničná 7, 128 44 Prague 2, Czech Republic.
authors.forename: Linda
authors.initials: L
authors.lastname: Nedbalová
authors.affiliation: Charles University in Prague, Faculty of Science, Department of Ecology, Viničná 7, 128 44 Prague 2, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Procházková
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20 Prague, Czech Republic.
authors.forename: Karel
authors.initials: K
authors.lastname: Sigler
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D008055:Lipids / Q000032:analysis* / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014280:Triglycerides / Q000032:analysis / Q000737:chemistry; D016823:Volvocida / Q000737:chemistry*
chemical_list: D008055:Lipids; D014280:Triglycerides; D012834:Silver
keywords: Chloromonas pichinchae; ESI; Lipidomics; NARP-LC/APCI-MS; Silver-LC/APCI-MS; Snow alga; Triacylglycerols