title: Development of a sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for the analysis of paclobutrazol residue in wheat kernel.
abstract: An indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed with monoclonal antibody (mAb) mAb6H73C9 recognizing the plant growth regulator paclobutrazol (PBZ). The icELISA had a half-maximum inhibition concentration (IC50) and working range of approximately 8.7 and 2.0-50.4 ng/mL, respectively. Average recoveries of PBZ in the wheat (Triticum aestivum) kernel samples were between 84.3 and 118.9% with relative standard deviations between 3.9 and 14.2%. As determined by the icELISA and further confirmed by liquid chromatography-electrospray ionization quadrupole Orbitrap mass spectrometry (LC-ESI-MS) analysis, the maximum residue concentration was about 0.07 mg/kg in the kernel samples, which indicated that PBZ could transfer from PBZ- treated seedlings to the kernel samples. The correlation coefficient (R(2)) between icELISA and LC-ESI-MS results was 0.979, which manifested that the developed icELISA was sensitive enough for monitoring PBZ residues in wheat kernels.
authors.affiliation: Engineering Research Center of Plant Growth Regulator, Ministry of Education, College of Agronomy and Biotechnology and §College of Science, China Agricultural University , Beijing 100193, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Cao
authors.forename: Hongwei
authors.initials: H
authors.lastname: Zhao
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Cui
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.forename: Guiyu
authors.initials: G
authors.lastname: Tan
authors.forename: Baomin
authors.initials: B
authors.lastname: Wang
authors.forename: Qing X
authors.initials: QX
authors.lastname: Li
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D014230:Triazoles / Q000032:analysis*; D014908:Triticum / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D010573:Pesticide Residues; D014230:Triazoles; C053370:paclobutrazol