title: Protective and antigenotoxic effect of Ulva rigida C. Agardh in experimental hypothyroid.
abstract: The presence of chromosomal damage in bone marrow cells affected by several diseases such as thyroid, cancer etc., was detected by the micronucleus (MN) assay. The present study was designed to evaluate: i) volatile components of Ulva rigida, ii) effects of hypothyroidism on bone marrow MN frequency, iii) effects of oral administration of Ulva rigida ethanolic extract (URE) on MN frequency produced by hypothyroidism, and iv) thyroid hormone levels in normal and 6-n-Propylthiouracil (PTU)-induced hypothyroid rats. The volatile components of Ulva rigida was studied using a direct thermal desorption (DTD) technique with comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GCxGC-TOF/MS). URE administration was of no significant impact on thyroid hormone levels in control group, while PTU administration decreased thyroid hormone levels compared to control group (p < 0.001). Moreover, URE supplementation resulted in a significant decrease in MN frequency in each thyroid group (p < 0.0001). This is the first in vivo study that shows the strong antigenotoxic and protective effect of URE against the genotoxicity produced by hypothyroidism.
authors.affiliation: Uludag University Department of Biology, Faculty of Science and Arts 16059 Bursa Turkey.
authors.forename: Serap
authors.initials: S
authors.lastname: Celikler
authors.affiliation: Uludag University Department of Biology, Faculty of Science and Arts 16059 Bursa Turkey.
authors.forename: Sibel
authors.initials: S
authors.lastname: Tas
authors.affiliation: Uludag University Department of Biology, Faculty of Science and Arts 16059 Bursa Turkey.
authors.forename: Sedef
authors.initials: S
authors.lastname: Ziyanok-Ayvalik
authors.affiliation: Uludag University Department of Biology, Faculty of Science and Arts 16059 Bursa Turkey.
authors.forename: O
authors.initials: O
authors.lastname: Vatan
authors.affiliation: Uludag University Department of Biology, Faculty of Science and Arts 16059 Bursa Turkey.
authors.forename: Gamze
authors.initials: G
authors.lastname: Yildiz
authors.affiliation: The University of York Department of Chemistry Heslington York UK YO10 5DD.
authors.forename: M
authors.initials: M
authors.lastname: Ozel
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects*; D004353:Drug Evaluation, Preclinical; D007037:Hypothyroidism / Q000188:drug therapy*; D008297:Male; D015162:Micronucleus Tests; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D044445:Ulva / Q000737:chemistry*
chemical_list: D010936:Plant Extracts
keywords: Ulva rigida; antigenotoxic effect; micronucleus; oxidative stress; thyroid; volatile compounds