title: IL-2 enhances cervical cancer cells proliferation and JAK3/STAT5 phosphorylation at low doses, while at high doses IL-2 has opposite effects.
abstract: The IL-2R signaling is critical for normal lymphocyte proliferation. However, the role of the IL-2 signaling in cervical cancer is not yet fully understood. We show that in IL-2R-expressing cervical cancer cells, JAK1 molecules are not phosphorylated. At low doses of IL-2, the constitutive phosphorylation of JAK3 and STAT5 increases in the tumor cells and decreases in lymphocytes, whereas the opposite occurs at high doses of IL-2. Using AG-490, the activation of JAK3 and the proliferation of cervical cancer cells were inhibited. We describe differences in the response of molecules downstream the IL-2R in lymphocytes and tumor cells.
authors.affiliation: Molecular Oncology Laboratory, Cell Differentiation and Cancer Research Unit, FES Zaragoza, National University of Mexico, Mexico City, Mexico;
authors.forename: Arturo
authors.initials: A
authors.lastname: Valle-Mendiola
authors.forename: Benny
authors.initials: B
authors.lastname: Weiss-Steider
authors.forename: Leticia
authors.initials: L
authors.lastname: Rocha-Zavaleta
authors.forename: Isabel
authors.initials: I
authors.lastname: Soto-Cruz
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D053613:Janus Kinase 1 / Q000378:metabolism; D053616:Janus Kinase 3 / Q000378:metabolism*; D008214:Lymphocytes / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D010766:Phosphorylation; D015375:Receptors, Interleukin-2 / Q000187:drug effects / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D050799:STAT5 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors; D002583:Uterine Cervical Neoplasms / Q000201:enzymology* / Q000473:pathology
chemical_list: D000970:Antineoplastic Agents; C508594:IL2 protein, human; D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins; D050799:STAT5 Transcription Factor; C507904:JAK1 protein, human; C507933:JAK3 protein, human; D053613:Janus Kinase 1; D053616:Janus Kinase 3