title: Isolation, biochemical and genetic characterizations of alcohol-producing yeasts from the flowers of Woodfordia fruticosa.
abstract: OBJECTIVE
  To isolate and characterize the alcohol-producing yeasts from Woodfordia fruticosa flowers, which are used for the induction and maintenance of fermentation in the making of Ayurvedic formulations.
  
  
  METHODS
  Initially twenty four yeasts strains were isolated on MGYP agar plate. Among them, four strains were selected for further studies on the basis of their alcohol generation capacity using jaggery media (50% w/v). Physiological, biochemical and genetic characterization (18S rRNA sequencing) of selected strains were carried out.
  
  
  RESULTS
  Physiological, biochemical and genetic characterization (18S rRNA sequencing) confirmed the strains as Saccharomycopsis fibuligera Jm.8, S. fibuligera Jm.10, S. fibuligera Jm.16 and Saccharomyces cerevisiae Jm.20. Under the controlled conditions, S. cerevisiae Jm.20 produced 69.57 g/l of alcohol, whereas remaining strains produced the alcohol in the range of 6.04-7.32 g/l.
  
  
  CONCLUSIONS
  Among selected strains, strains S. fibuligera are a newer in the flowers. Kinetic study of alcohol generation revealed the strain S. cerevisiae Jm.20 can be efficiently used in making of fermented Ayurvedic formulations instead of use W. fruticosa flowers.
authors.affiliation: Poona College of Pharmacy, Bharati Vidyapeeth University, Pune 411 038, India.
authors.forename: Jagdish
authors.initials: J
authors.lastname: Manwar
authors.affiliation: Poona College of Pharmacy, Bharati Vidyapeeth University, Pune 411 038, India.
authors.forename: Kakasaheb
authors.initials: K
authors.lastname: Mahadik
authors.affiliation: Centre for Pharmaceutical Engineering Science, University of Bradford, Bradford BD7 1DP, UK.
authors.forename: Anant
authors.initials: A
authors.lastname: Paradkar
authors.affiliation: Poona College of Pharmacy, Bharati Vidyapeeth University, Pune 411 038, India.
authors.forename: Lohidasan
authors.initials: L
authors.lastname: Sathiyanarayanan
authors.affiliation: Department of Alcohol Technology, Vasantdada Sugar Institute, Pune 412 307, India.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Vohra
authors.affiliation: Department of Alcohol Technology, Vasantdada Sugar Institute, Pune 412 307, India.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Patil
keywords: Alcohol; Saccharomyces cerevisiae Jm.20; Woodfordia fruticosa; Yeast