title: A versatile ultra-high performance LC-MS method for lipid profiling.
abstract: A new UPLC-based untargeted lipidomic approach using a qTOF hybrid mass spectrometer is introduced. The applied binary gradient enables separations of lipid species including constitutional isomeric compounds and low abundant lipid classes such as phosphatidic acid (PA). Addition of phosphoric acid to the solvents improves peak shapes for acidic phospholipids. MS(E) scans allow simultaneous acquisition of full scan data and collision induced fragmentation to improve identification of lipid classes and to obtain structural information. The method was used to investigate the lipidome of yeast.
authors.affiliation: Institute of Molecular Biosciences, University of Graz, Humboldtstraße 50/II, 8010 Graz, Austria.
authors.forename: Oskar L
authors.initials: OL
authors.lastname: Knittelfelder
authors.affiliation: Institute of Molecular Biosciences, University of Graz, Humboldtstraße 50/II, 8010 Graz, Austria.
authors.forename: Bernd P
authors.initials: BP
authors.lastname: Weberhofer
authors.affiliation: Institute of Molecular Biosciences, University of Graz, Humboldtstraße 50/II, 8010 Graz, Austria.
authors.forename: Thomas O
authors.initials: TO
authors.lastname: Eichmann
authors.affiliation: Institute of Molecular Biosciences, University of Graz, Humboldtstraße 50/II, 8010 Graz, Austria.
authors.forename: Sepp D
authors.initials: SD
authors.lastname: Kohlwein
authors.affiliation: Institute of Molecular Biosciences, University of Graz, Humboldtstraße 50/II, 8010 Graz, Austria; Omics Center Graz, Austria. Electronic address: gerald.rechberger@uni-graz.at.
authors.forename: Gerald N
authors.initials: GN
authors.lastname: Rechberger
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D020404:Glycerophospholipids; D008055:Lipids / Q000032:analysis* / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D009132:Muscles / Q000737:chemistry; D010756:Phosphoric Acids; D015003:Yeasts / Q000737:chemistry
chemical_list: D020404:Glycerophospholipids; D008055:Lipids; D010756:Phosphoric Acids; C030242:phosphoric acid
keywords: Glycerophospholipids; Lipidomics; Mass spectrometry; Neutral lipids; Ultra-performance liquid chromatography