title: Mesenchymal stem cells ameliorate Th1-induced pre-eclampsia-like symptoms in mice via the suppression of TNF-α expression.
abstract: Pre-eclampsia (PE) is thought to be a pregnancy-induced autoimmune disease. Despite several strategies carried out for targeting specific factors relevant to its pathogenesis, PE remains potentially fatal to some patients. Here, we reported a way to isolate mesenchymal stem cells (MSCs) from decidua. The MSCs not only exhibited differentiation and self-renewal capacities, they also possessed immunomodulatory functions and secreted some soluble mediators including IL-6, TGF-β, IDO, VEGF and COX-2. Most importantly, the MSCs were specifically provided with the ability to suppress T cells proliferation by IDO in response to inflammatory cytokine IFN-γ. Moreover, we developed a Th1 cell-induced PE mouse model which displayed a high level of pathogenesis factor TNF-α. Strikingly, MSCs-based therapy significantly ameliorated both clinical and histopathological severity of PE symptoms including decreasing the blood pressure and proteinuria, suppressing glomerulonephritis, protecting the feto-placental development. The therapy also reversed abnormal TNF-α expression in uterine and splenic lymphocytes. These data suggest that MSCs may ameliorate Th1-induced PE-like symptoms in mice via the suppression of TNF-α and MSCs-based therapy may provide a potential novel method for PE.
authors.affiliation: Immunology Lab, Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Liu
authors.affiliation: Immunology Lab, Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, China.
authors.forename: Guangfeng
authors.initials: G
authors.lastname: Zhao
authors.affiliation: Immunology Lab, Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, China.
authors.forename: Hongye
authors.initials: H
authors.lastname: Fan
authors.affiliation: Immunology Lab, Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, China.
authors.forename: Xiaoyin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Immunology Lab, Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Li
authors.affiliation: The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, China.
authors.forename: Zhiqun
authors.initials: Z
authors.lastname: Wang
authors.affiliation: The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Immunology Lab, Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, China ; Jiangsu Key Laboratory of Molecular Medicine, Nanjing, China.
authors.forename: Yayi
authors.initials: Y
authors.lastname: Hou
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000818:Animals; D001794:Blood Pressure; D002454:Cell Differentiation; D049109:Cell Proliferation; D004195:Disease Models, Animal; D005260:Female; D005921:Glomerulonephritis / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010012:Osteogenesis; D011225:Pre-Eclampsia / Q000473:pathology*; D011247:Pregnancy; D011270:Pregnancy, Animal; D013601:T-Lymphocytes / Q000166:cytology; D018417:Th1 Cells / Q000166:cytology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D014470:Umbilical Cord / Q000378:metabolism
chemical_list: D014409:Tumor Necrosis Factor-alpha