title: Effects of 17α-ethinyl estradiol exposure on estrogen receptors α and β and vitellogenins A, B and C mRNA expression in the liver of sand goby (Pomatoschistus minutus).
abstract: This study aims to characterize the estrogen receptor (er) in sand goby (Pomatoschistus minutus) and determine the temporal effects of 17α-ethinyl estradiol (EE2) on erα and vitellogenin (vtg) gene expression in males. Two partial cDNA sequences (erα and erβ1) are presented showing conserved structural features with ers of other species. Transcript levels for both ers were low in control fish but EE2 exposure (11 ng/L, for 29 days) increased both to a pattern similar to vitellogenic females. The relative expression of three vtg genes (vtga, vtgb and vtgc) along with erα was determined in control and male fish exposed to EE2 (11 ng/L) at multiple time-points over 29 days. All four transcripts were significantly induced due to exposure and expression rose during the time course with distinct temporal patterns and vtga reached a substantially higher level at the end of the time course coinciding with rapid elevation in erα expression.
authors.affiliation: School of Health and Life Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow G4 0BA, United Kingdom.
authors.forename: Joseph L
authors.initials: JL
authors.lastname: Humble
authors.affiliation: Department of Bio and Environmental Sciences, University of Helsinki, Finland.
authors.forename: Minna
authors.initials: M
authors.lastname: Saaristo
authors.affiliation: Department of Environmental and Marine Biology, Åbo Akademi University, Finland.
authors.forename: Kai
authors.initials: K
authors.lastname: Lindström
authors.affiliation: Marine Research Centre, Finnish Environment Institute, P.O.B. 140, 00251 Helsinki, Finland.
authors.forename: Kari K
authors.initials: KK
authors.lastname: Lehtonen
authors.affiliation: School of Health and Life Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow G4 0BA, United Kingdom. Electronic address: j.a.craft@gcu.ac.uk.
authors.forename: John A
authors.initials: JA
authors.lastname: Craft
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004781:Environmental Exposure; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism; D047629:Estrogen Receptor beta / Q000235:genetics / Q000378:metabolism; D004997:Ethinyl Estradiol / Q000633:toxicity*; D005260:Female; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D010473:Perciformes / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D014819:Vitellogenins / Q000235:genetics / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D029941:Fish Proteins; D012333:RNA, Messenger; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D004997:Ethinyl Estradiol
keywords: 17α-Ethinyl estrodiol; Endocrine disruption; Estrogen receptor; Pomatoschistus minutus; Sand goby; Transcript abundance; Vitellogenin; qPCR