title: Complete genome sequence of the cyanide-degrading bacterium Pseudomonas pseudoalcaligenes CECT5344.
abstract: Pseudomonas pseudoalcaligenes CECT5344, a Gram-negative bacterium isolated from the Guadalquir River (Córdoba, Spain), is able to utilize different cyano-derivatives. Here, the complete genome sequence of P. pseudoalcaligenes CECT5344 harboring a 4,686,340bp circular chromosome encoding 4513 genes and featuring a GC-content of 62.34% is reported. Necessarily, remaining gaps in the genome had to be closed by assembly of few long reads obtained from PacBio single molecule real-time sequencing. Here, the first complete genome sequence for the species P. pseudoalcaligenes is presented.
authors.affiliation: Center for Biotechnology, Bielefeld University, 33615 Bielefeld, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Wibberg
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, Córdoba, Spain.
authors.forename: Víctor M
authors.initials: VM
authors.lastname: Luque-Almagro
authors.affiliation: Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain.
authors.forename: Ma Isabel
authors.initials: MI
authors.lastname: Igeño
authors.affiliation: Center for Biotechnology, Bielefeld University, 33615 Bielefeld, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Bremges
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, Córdoba, Spain.
authors.forename: Ma Dolores
authors.initials: MD
authors.lastname: Roldán
authors.affiliation: Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain.
authors.forename: Faustino
authors.initials: F
authors.lastname: Merchán
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, Córdoba, Spain.
authors.forename: Lara P
authors.initials: LP
authors.lastname: Sáez
authors.affiliation: Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain.
authors.forename: Ma Isabel
authors.initials: MI
authors.lastname: Guijo
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, Córdoba, Spain.
authors.forename: Ma Isabel
authors.initials: MI
authors.lastname: Manso
authors.affiliation: Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Macías
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, Córdoba, Spain.
authors.forename: Purificación
authors.initials: P
authors.lastname: Cabello
authors.affiliation: Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain.
authors.forename: Gracia
authors.initials: G
authors.lastname: Becerra
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, Córdoba, Spain.
authors.forename: Ma Isabel
authors.initials: MI
authors.lastname: Ibáñez
authors.affiliation: Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain.
authors.forename: Ma Isabel
authors.initials: MI
authors.lastname: Carmona
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, Córdoba, Spain.
authors.forename: Ma María Paz
authors.initials: MM
authors.lastname: Escribano
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, Córdoba, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Castillo
authors.affiliation: Center for Biotechnology, Bielefeld University, 33615 Bielefeld, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Sczyrba
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, Córdoba, Spain.
authors.forename: Conrado
authors.initials: C
authors.lastname: Moreno-Vivián
authors.affiliation: Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Blasco
authors.affiliation: Center for Biotechnology, Bielefeld University, 33615 Bielefeld, Germany.
authors.forename: Alfred
authors.initials: A
authors.lastname: Pühler
authors.affiliation: Center for Biotechnology, Bielefeld University, 33615 Bielefeld, Germany. Electronic address: aschluet@cebitec.uni-bielefeld.de.
authors.forename: Andreas
authors.initials: A
authors.lastname: Schlüter
mesh_terms: D001483:Base Sequence; D002876:Chromosomes, Bacterial; D003486:Cyanides / Q000378:metabolism*; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D044205:Pseudomonas pseudoalcaligenes / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA
chemical_list: D003486:Cyanides
keywords: Bioplastic; Cyanide; Cyanide assimilation; Cyanide resistance; Nitrilase