title: Using oxidant and antioxidant levels to predict the duration of both acute peripheral and mesenteric ischemia.
abstract: OBJECTIVE
  The aim of this study was to determine the relationship between oxidative stress markers and the duration of ischemia in rat mesenteric and peripheral ischemia models.
  
  
  METHODS
  Forty rats were divided into five equal groups, as follows: rats in Group I (control group) were sacrificed to determine the baseline characteristics of the serum markers; the superior mesenteric artery was clamped via a simple laparotomy to induce mesenteric ischemia in Groups II and III; the right common femoral artery was clamped to induce peripheral ischemia in Groups IV and V. Blood samples were taken at 2 (Groups II and IV) and 6 (Groups III and V) hours after these procedures. The serum total oxidative status (TOS), total antioxidant capacity (TAC), oxidative stress index (OSI) and paraoxonase-1 (PON-1) enzyme activities were evaluated in the samples obtained from each group.
  
  
  RESULTS
  The OSI level of the control group was 91.00±5.46 (mean ± SD). The OSI levels taken 2 hours after the induction of mesenteric ischemia and peripheral ischemia were significantly higher (194.50±11.16 and 301.75±19.98, respectively (p<0.05)). However, these levels decreased to 151.88±17.02 (mesenteric ischemia) and 108.88±9.46 (peripheral ischemia) after 6 hours. The PON-1 levels of Group III (mesenteric ischemia at 6 hours) (99.75±7.26), Group IV (peripheral ischemia at 2 hours) (96.88±4.09), and Group V (peripheral ischemia at 6 hours) (111.25±10.33) were slightly elevated over that of the control group (87.38±5.31). However, the PON-1 level of Group II (mesenteric ischemia at 2 hours) (42.88±3.14) was lower than that of the other groups (p<0.05).
  
  
  CONCLUSIONS
  Despite the increment of oxidative markers in early periods of ischemia (2(nd) hour), which was a hypoxic response of ischemic cells, they have decreased markedly in prolonged ischemia. This might have been caused by the opening of some collateral circulation or the destruction of the ischemic cells.
authors.affiliation: Department of Cardiovascular Surgery, Medical School of Dicle University, Diyarbakir, Turkey yazici.suleyman@hotmail.com.
authors.forename: S
authors.initials: S
authors.lastname: Yazici
authors.affiliation: Department of Cardiovascular Surgery, Medical School of Dicle University, Diyarbakir, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Demirtas
authors.affiliation: Department of Cardiovascular Surgery, Medical School of Dicle University, Diyarbakir, Turkey.
authors.forename: O
authors.initials: O
authors.lastname: Guclu
authors.affiliation: Department of Cardiovascular Surgery, Medical School of Dicle University, Diyarbakir, Turkey.
authors.forename: O
authors.initials: O
authors.lastname: Karahan
authors.affiliation: Department of Cardiovascular Surgery, Medical School of Dicle University, Diyarbakir, Turkey.
authors.forename: C
authors.initials: C
authors.lastname: Yavuz
authors.affiliation: Department of Cardiovascular Surgery, Medical School of Dicle University, Diyarbakir, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Caliskan
authors.affiliation: Department of Cardiovascular Surgery, Medical School of Dicle University, Diyarbakir, Turkey.
authors.forename: B
authors.initials: B
authors.lastname: Mavitas
mesh_terms: D000208:Acute Disease; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D043303:Aryldialkylphosphatase / Q000378:metabolism; D007511:Ischemia / Q000097:blood* / Q000503:physiopathology; D008297:Male; D008643:Mesentery / Q000098:blood supply*; D016877:Oxidants / Q000097:blood*; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D013152:Splanchnic Circulation; D013997:Time Factors
chemical_list: D000975:Antioxidants; D016877:Oxidants; C572204:Pon1 protein, rat; D043303:Aryldialkylphosphatase
keywords: OSI levels; PON-1 levels; ischemia duration; non-cardiac ischemia; oxidative balance