title: Strigolactone-regulated proteins revealed by iTRAQ-based quantitative proteomics in Arabidopsis.
abstract: Strigolactones (SLs) are a new class of plant hormones. In addition to acting as a key inhibitor of shoot branching, SLs stimulate seed germination of root parasitic plants and promote hyphal branching and root colonization of symbiotic arbuscular mycorrhizal fungi. They also regulate many other aspects of plant growth and development. At the transcription level, SL-regulated genes have been reported. However, nothing is known about the proteome regulated by this new class of plant hormones. A quantitative proteomics approach using an isobaric chemical labeling reagent, iTRAQ, to identify the proteome regulated by SLs in Arabidopsis seedlings is presented. It was found that SLs regulate the expression of about three dozen proteins that have not been previously assigned to SL pathways. These findings provide a new tool to investigate the molecular mechanism of action of SLs.
authors.affiliation: Chemical Sciences Division, ‡Biosciences Division, and §Computer Science and Mathematics Division, Oak Ridge National Laboratory , 1 Bethel Valley Road, Oak Ridge, Tennessee 37831, United States.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Li
authors.forename: Olaf
authors.initials: O
authors.lastname: Czarnecki
authors.forename: Karuna
authors.initials: K
authors.lastname: Chourey
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Gerald A
authors.initials: GA
authors.lastname: Tuskan
authors.forename: Gregory B
authors.initials: GB
authors.lastname: Hurst
authors.forename: Chongle
authors.initials: C
authors.lastname: Pan
authors.forename: Jin-Gui
authors.initials: JG
authors.lastname: Chen
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D005658:Fungi / Q000187:drug effects / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D018525:Germination / Q000187:drug effects; D007783:Lactones / Q000494:pharmacology*; D058977:Molecular Sequence Annotation; D038821:Mycorrhizae / Q000187:drug effects / Q000502:physiology; D010937:Plant Growth Regulators / Q000494:pharmacology*; D040901:Proteomics / Q000295:instrumentation / Q000379:methods; D036226:Seedlings / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D012639:Seeds / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D013194:Staining and Labeling
chemical_list: D029681:Arabidopsis Proteins; D007783:Lactones; D010937:Plant Growth Regulators