title: Analysis of TP53 gene expression and p53 level of human hypopharyngeal FaDu (HTB-43) head and neck cancer cell line after microRNA-181a inhibition.
abstract: The identification of new biomarkers for early detection of highly recurrent head and neck cancer is urgently needed. MicroRNAs (miRNAs) are small and non-coding RNAs that regulate cancer-related gene expression, such as tumor protein 53 (TP53) gene expression. This study was carried out to analyze TP53 gene expression using real-time PCR and to determine changes in intracellular p53 level by flow cytometry after downregulation of miRNA-181a miRNA inhibitor in the FaDu cell line. TP53 gene expression showed a 3-fold increment and the p53 protein level was also increased in the miRNA-181a-treated cells. In conclusion, miRNA-181a binds to the TP53 gene and inhibits its expression, decreasing the synthesis of p53.
authors.affiliation: Faculty of Medicine and Health Sciences, University Putra Malaysia, Serdang, Selangor, Malaysia ykcheah@medic.upm.edu.my.
authors.forename: Y K
authors.initials: YK
authors.lastname: Cheah
authors.affiliation: Faculty of Medicine and Health Sciences, University Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: R W
authors.initials: RW
authors.lastname: Cheng
authors.affiliation: Institute of Bioscience, University Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: S K
authors.initials: SK
authors.lastname: Yeap
authors.affiliation: Faculty of Medicine and Health Sciences, University Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: C H
authors.initials: CH
authors.lastname: Khoo
authors.affiliation: Biomarketing Services (M) Sdn. Bhd., Bandar Menjalara, Kuala Lumpur, Malaysia.
authors.forename: H S
authors.initials: HS
authors.lastname: See
mesh_terms: D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000473:pathology; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics*; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C540193:MIrn181 microRNA, human; D035683:MicroRNAs; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53