title: Expression of synaptopodin in endothelial cells exposed to laminar shear stress and its role in endothelial wound healing.
abstract: We examined the hypothesis that certain actin binding proteins might be upregulated by laminar shear stress (LSS) and could contribute to endothelial wound healing. Analysis of mRNA expression profiles of human umbilical vein endothelial cells under static and LSS-exposed conditions provided a list of LSS-induced actin binding proteins including synaptopodin (SYNPO) whose endothelial expression has not been previously reported. Additional studies demonstrated that SYNPO is a key mediator of endothelial wound healing because small interfering RNA-mediated suppression of SYNPO attenuated wound closure under LSS whereas overexpression of exogenous SYNPO enhanced endothelial wound closure in the absence of LSS. This study suggests that LSS-induced actin binding proteins including SYNPO may play a critical role in the endothelial wound healing stimulated by LSS.
authors.affiliation: Department of Molecular Medicine, Cell and Matrix Research Institute, BK21 Plus KNU Biomedical Convergence Program, Kyungpook National University School of Medicine, Daegu, Republic of Korea.
authors.forename: Gyeong In
authors.initials: GI
authors.lastname: Mun
authors.affiliation: Department of Molecular Medicine, Cell and Matrix Research Institute, BK21 Plus KNU Biomedical Convergence Program, Kyungpook National University School of Medicine, Daegu, Republic of Korea.
authors.forename: Soojin
authors.initials: S
authors.lastname: Park
authors.affiliation: Internal Medicine D, Department of Nephrology, Hypertension and Rheumatology, University Hospital Muenster, Muenster, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Kremerskothen
authors.affiliation: Department of Molecular Medicine, Cell and Matrix Research Institute, BK21 Plus KNU Biomedical Convergence Program, Kyungpook National University School of Medicine, Daegu, Republic of Korea. Electronic address: ycboo@knu.ac.kr.
authors.forename: Yong Chool
authors.initials: YC
authors.lastname: Boo
mesh_terms: D001696:Biomechanical Phenomena; D002465:Cell Movement; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology; D061307:Human Umbilical Vein Endothelial Cells / Q000502:physiology*; D006801:Humans; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological; D059467:Transcriptome; D014945:Wound Healing
chemical_list: D008840:Microfilament Proteins; C503967:SYNPO protein, human
keywords: Endothelial cell; Shear stress; Synaptopodin; Wound healing