title: Genotype I of hepatitis B virus was found in east Xishuangbanna, China and molecular dynamics of HBV/I.
abstract: There is a dearth of data about the prevalence of hepatitis B virus (HBV) infection in Mengla, China; and no detailed analysis of the molecular evolution of genotype I in Asia. In this study, 909 serum samples from ethnic minority people in China were obtained. Serological assay and HBV S-gene amplification were carried out, and phylogenetic and evolutionary dynamics analysis of 62 HBV/I S-gene was performed. On this survey, 153 individuals were tested HBsAg-positive. Genotypes of S-gene were classified into three groups: C, B and I. Under the strict model and the relax model, the estimated evolutionary rates for HBV/I were 3.74 × 10(-4) and 6.93 × 10(-4) substitution/site/year, respectively. However, when the geographic origin was taken into account, the mean substitution rates were increased. Estimated time to most recent ancestor of genotype I varied from ~30 to ~70 years ago. The Bayesian sky plot showed a rapid spread of HBV/I at the end of 1980s. Peculiar nucleotides distributed were observed in the subgenotype I1/I2. In conclusion, higher prevalence of HBV infection was observed in Mengla county. Multifactors like timescale and spatial locations should be integrated to provide a better interpretation of the HBV/I evolutionary history in the region.
authors.affiliation: Medical Science College of Yunnan University, Kunming, China; Institute of Basic and Clinical Medicine, Center of Clinical Molecular Biology, Provincial Key Laboratory for Birth Defects and Genetic Diseases, The First People's Hospital of Yunnan Province, Affiliated Hospital of Kunming Science and Technology University, Kunming, China.
authors.forename: T
authors.initials: T
authors.lastname: Shen
authors.forename: X M
authors.initials: XM
authors.lastname: Yan
authors.forename: H X
authors.initials: HX
authors.lastname: Liu
authors.forename: B X
authors.initials: BX
authors.lastname: Zhang
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.forename: J P
authors.initials: JP
authors.lastname: Zhang
authors.forename: J L
authors.initials: JL
authors.lastname: Wang
authors.forename: C J
authors.initials: CJ
authors.lastname: Xiao
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D005260:Female; D005838:Genotype; D006509:Hepatitis B / Q000453:epidemiology* / Q000821:virology*; D006514:Hepatitis B Surface Antigens / Q000097:blood / Q000235:genetics; D006515:Hepatitis B virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D058974:Phylogeography; D015995:Prevalence; D017422:Sequence Analysis, DNA; D012698:Serologic Tests; D055815:Young Adult
chemical_list: D006514:Hepatitis B Surface Antigens
keywords: Bayesian analysis; hepatitis B virus; nucleotide substitution rate; time to most recent common ancestor