title: Paneth cells are also target of the ribotoxic lectin nigrin b.
abstract: Ribosome-inactivating lectins (RILs) are A-B type toxins like ricin whose molecular target is the large rRNA of eukaryotic ribosome. Administration of lethal doses of the RIL nigrin b isolated from elderberry (Sambucus nigra L.) bark triggers specific intestinal derangement. The aim of the present research was to explore the early effects of a lethal dose of nigrin b (16 mg/kg body weight) on the small intestine using light and electron microscopy to ascertain intestinal epithelium changes. 6 h after nigrin administration, the small intestine crypts began to show signs of damage with cells appearing at different stages of apoptosis. 16 h after injection crypts appeared more impaired, including the derangement of Paneth cells. The novelty of our results is that the Paneth cells in the small intestine in addition to stem cells are the early cellular targets for nigrin b.
authors.affiliation: Department of Nutrition and Food Sciences, Faculty of Medicine, and CINAD. University of Valladolid, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Jiménez
authors.affiliation: Department of Cell Biology, Histology and Pharmacology, Faculty of Medicine, and INCYL, University of Valladolid, Spain.
authors.forename: Manuel José
authors.initials: MJ
authors.lastname: Gayoso
authors.affiliation: Department of Cell Biology, Histology and Pharmacology, Faculty of Medicine, and INCYL, University of Valladolid, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Garrosa
authors.affiliation: Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, and IUFI, Complutense University of Madrid, Spain.
authors.forename: Damián
authors.initials: D
authors.lastname: Córdoba-Díaz
authors.affiliation: Department of Nutrition and Food Sciences, Faculty of Medicine, and CINAD. University of Valladolid, Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: Cabrero
authors.affiliation: Department of Nutrition and Food Sciences, Faculty of Medicine, and CINAD. University of Valladolid, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Tejero
authors.affiliation: Department of Nutrition and Food Sciences, Faculty of Medicine, and CINAD. University of Valladolid, Spain.
authors.forename: Mónica
authors.initials: M
authors.lastname: Aracil
authors.affiliation: Department of Nutrition and Food Sciences, Faculty of Medicine, and CINAD. University of Valladolid, Spain. girbes@bio.uva.es.
authors.forename: Tomás
authors.initials: T
authors.lastname: Girbés
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D005260:Female; D007421:Intestine, Small / Q000187:drug effects / Q000473:pathology; D051379:Mice; D019879:Paneth Cells / Q000187:drug effects* / Q000473:pathology; D010940:Plant Proteins / Q000633:toxicity*; D054788:Ribosome Inactivating Proteins / Q000633:toxicity*
chemical_list: D010940:Plant Proteins; C579416:nigrin b protein, Sambucus nigra; D054788:Ribosome Inactivating Proteins