title: Exposition to tannery wastewater did not alter behavioral and biochemical parameters in Wistar rats.
abstract: There are scarce data on the neurotoxicity in mammalian induced by tannery wastewaters. Previously, the anxiogenic effect of tannery wastewater was demonstrated in mice, while wastewater submitted to photoelectrooxidation (PEO) process treatment did not affect the anxiety state. Considering that species may response differently to xenobiotics, the aim of the present work was to study the effects of exposure to tannery wastewaters (non-PEO or PEO-treated) on behavioral and neurochemical markers in another species of laboratory animals, specifically Wistar rats. Male Wistar rats were given free access to water bottles containing non-PEO or PEO-treated tannery wastewaters (0.1, 1 and 5% in drinking water). During the exposure, behavioral tests of anxiety (elevated plus-maze, neophobia, open field and light-dark box), depression (forced swimming) and memory (inhibitory avoidance, novel object and discriminative avoidance) were performed. On the 30th day, brain structures were dissected out to evaluate cellular oxidative state (hippocampus, cerebellum and striatum) and acetylcholinesterase activity (hippocampus and striatum). Exposure to tannery effluent with or without photoelectrochemical treatment did not alter any behavioral and neurochemical parameters evaluated. Our data indicate that Wistar rats may not be an adequate species for ecotoxicological studies involving tannery effluents and that POE treatment did not generate other toxic compounds.
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Felipe dos Santos
authors.initials: Fdos S
authors.lastname: Moysés
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Karine
authors.initials: K
authors.lastname: Bertoldi
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Christiano
authors.initials: C
authors.lastname: Spindler
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Neurociências, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Eduardo Farias
authors.initials: EF
authors.lastname: Sanches
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Viviane Rostirola
authors.initials: VR
authors.lastname: Elsner
authors.affiliation: Universidade FEEVALE, Novo Hamburgo, RS, Brazil.
authors.forename: Marco Antonio Siqueira
authors.initials: MA
authors.lastname: Rodrigues
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil; Unidade de Experimentação Animal, Grupo de Pesquisa e Pós-Graduação do Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil. Electronic address: ionara@ufrgs.br.
authors.forename: Ionara Rodrigues
authors.initials: IR
authors.lastname: Siqueira
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001007:Anxiety / Q000139:chemically induced; D001522:Behavior, Animal / Q000187:drug effects*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism*; D002531:Cerebellum / Q000187:drug effects / Q000378:metabolism; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism; D003866:Depressive Disorder / Q000139:chemically induced; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D007220:Industrial Waste; D008297:Male; D008568:Memory / Q000187:drug effects; D009483:Neuropsychological Tests; D010084:Oxidation-Reduction / Q000187:drug effects; D017208:Rats, Wistar; D062065:Wastewater
chemical_list: D000110:Acetylcholinesterase; D007220:Industrial Waste; D062065:Wastewater
keywords: Neurotoxicity; Photoelectrooxidation process; Rats; Tannery effluents