title: A highly effective TALEN-mediated approach for targeted gene disruption in Xenopus tropicalis and zebrafish.
abstract: Transcription activator like effector nucleases (TALENs) is a promising approach to disrupt intended genomic loci. The assembly of highly effective TALENs is critical for successful genome editing. Recently we reported a convenient and robust platform to construct customized TALENs. The TALENs generated by this platform have been proven to be highly effective for gene disruption in Xenopus tropicalis and zebrafish as well as large genomic deletions in zebrafish. The one-time success rate of targeted gene disruption is about 90% for more than 100 genomic loci tested, with the mutation frequencies often reaching above 50%. Here we describe the validated protocol for TALEN assembly, methods for generating gene knockout animals in X. tropicalis and zebrafish, as well as the protocol for engineering large genomic deletions in zebrafish.
authors.affiliation: School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, China; School of Biomedical Sciences Core Laboratory, The Chinese University of Hong Kong Shenzhen Research Institute, Shenzhen 518057, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China; Department of Genetics, School of Basic Medical Sciences, Wuhan University, Wuhan, China.
authors.forename: Daji
authors.initials: D
authors.lastname: Luo
authors.affiliation: School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, China; School of Biomedical Sciences Core Laboratory, The Chinese University of Hong Kong Shenzhen Research Institute, Shenzhen 518057, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Lei
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China. Electronic address: huwei@ihb.ac.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
authors.affiliation: School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, China; School of Biomedical Sciences Core Laboratory, The Chinese University of Hong Kong Shenzhen Research Institute, Shenzhen 518057, China. Electronic address: zhaohui@cuhk.edu.hk.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.affiliation: School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, China; School of Biomedical Sciences Core Laboratory, The Chinese University of Hong Kong Shenzhen Research Institute, Shenzhen 518057, China. Electronic address: chkcheng@cuhk.edu.hk.
authors.forename: Christopher H K
authors.initials: CH
authors.lastname: Cheng
mesh_terms: D000818:Animals; D003851:Deoxyribonucleases / Q000235:genetics; D017353:Gene Deletion; D055786:Gene Knockout Techniques; D005818:Genetic Engineering / Q000379:methods; D016678:Genome; D008845:Microinjections; D014981:Xenopus / Q000196:embryology / Q000235:genetics*; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*
chemical_list: D003851:Deoxyribonucleases
keywords: Gene knockout; Genomic deletions; TALENs; Xenopus; Zebrafish