title: Identification and characterization of a multi-domain sulfurtransferase in Phanerochaete chrysosporium.
abstract: A sulfurtransferase gene (PcSft) with a coding region of 546 bp was cloned from the filamentous white-rot fungus Phanerochaere chrysosporium. The 181-amino acid protein contains a highly conserved "Rhodanese-like" domain and an ATP-binding site, with a molecular weight of 20.68 kDa. Semi-quantitative RT-PCR showed that the selective expression of PcSft was involved in secondary metabolism. The recombinant PcSFT protein was expressed in E. coli BL21 (DE3) and purified by Ni(2+)-chelating and size-exclusion chromatography. Its ATPase and sulfurtransferase (SFT) activities were indentified and characterized. PcSFT exhibited optimal SFT activity at pH 8 and 30 °C as well as stability at 20 °C and pH 8. The enzyme's stability under different temperature and pH P. indicates a potential usefulness for the detoxification of cyanide in the environment.
authors.affiliation: Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, 29 Wangjiang Road, Chengdu, 610064, Sichuan Province, China.
authors.forename: Zhongshan
authors.initials: Z
authors.lastname: Wang
authors.forename: Guangjun
authors.initials: G
authors.lastname: Wang
authors.forename: Quanju
authors.initials: Q
authors.lastname: Xiang
authors.forename: Yizheng
authors.initials: Y
authors.lastname: Zhang
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
mesh_terms: D000251:Adenosine Triphosphatases; D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D020075:Phanerochaete / Q000201:enzymology* / Q000235:genetics*; D017434:Protein Structure, Tertiary / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D013466:Sulfurtransferases / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D011994:Recombinant Proteins; D013466:Sulfurtransferases; D000251:Adenosine Triphosphatases