title: Method for obtaining primary ovarian cancer cells from solid specimens.
abstract: Reliable tools for investigating ovarian cancer initiation and progression are urgently needed. While the use of ovarian cancer cell lines remains a valuable tool for understanding ovarian cancer, their use has many limitations. These include the lack of heterogeneity and the plethora of genetic alterations associated with extended in vitro passaging. Here we describe a method that allows for rapid establishment of primary ovarian cancer cells form solid clinical specimens collected at the time of surgery. The method consists of subjecting clinical specimens to enzymatic digestion for 30 min. The isolated cell suspension is allowed to grow and can be used for downstream application including drug screening. The advantage of primary ovarian cancer cell lines over established ovarian cancer cell lines is that they are representative of the original specific clinical specimens they are derived from and can be derived from different sites whether primary or metastatic ovarian cancer.
authors.affiliation: Department of Obstetrics, Gynecology, and Women's Heath, University of Minnesota.
authors.forename: Lee J
authors.initials: LJ
authors.lastname: Pribyl
authors.affiliation: Department of Obstetrics, Gynecology, and Women's Heath, University of Minnesota.
authors.forename: Kathleen A
authors.initials: KA
authors.lastname: Coughlin
authors.affiliation: Department of Obstetrics, Gynecology, and Women's Heath, University of Minnesota.
authors.forename: Thanasak
authors.initials: T
authors.lastname: Sueblinvong
authors.affiliation: Department of Obstetrics and Gynecology, Maricopa Medical Center and St Josephs Hospital and Medical Center.
authors.forename: Kristin
authors.initials: K
authors.lastname: Shields
authors.affiliation: Department of Obstetrics, Gynecology, and Women's Heath, University of Minnesota; Masonic Cancer Center, University of Minnesota.
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Iizuka
authors.affiliation: Department of Obstetrics, Gynecology, and Women's Heath, University of Minnesota; Masonic Cancer Center, University of Minnesota.
authors.forename: Levi S
authors.initials: LS
authors.lastname: Downs
authors.affiliation: Department of Obstetrics, Gynecology, and Women's Heath, University of Minnesota; Masonic Cancer Center, University of Minnesota.
authors.forename: Rahel G
authors.initials: RG
authors.lastname: Ghebre
authors.affiliation: Department of Obstetrics, Gynecology, and Women's Heath, University of Minnesota; Masonic Cancer Center, University of Minnesota; mbazzaro@umn.edu.
authors.forename: Martina
authors.initials: M
authors.lastname: Bazzaro
mesh_terms: D000077216:Carcinoma, Ovarian Epithelial; D045744:Cell Line, Tumor; D003584:Cytological Techniques / Q000379:methods; D005260:Female; D006801:Humans; D009375:Neoplasms, Glandular and Epithelial / Q000473:pathology*; D010051:Ovarian Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured