title: Elevated frequencies of micronuclei in pregnant women with type 1 diabetes mellitus and in their newborns.
abstract: Pregestational diabetes mellitus (type 1 and type 2) affects about 1% of the obstetric population. In diabetes, persistent hyperglycemia can be a source of DNA damage via overproduction of reactive oxygen species (ROS). Using the cytokinesis-block micronucleus (CBMN) test, we measured the frequencies of micronuclei (MN) per 1000 binucleated (BN) cells in pregnant women (mothers) with type 1 diabetes mellitus (T1DM) and in their newborns. Peripheral blood lymphocytes were collected from 17 pregnant women with T1DM and cord-blood lymphocytes from their 17 newborns. The control group included 40 pregnant women (mothers) without diabetes mellitus (DM) and their 40 newborns. In the group of pregnant women with T1DM, the mean number of MN per 1000 BN cells was 2.35 (±1.07), significantly (p<0.001) higher than in the control group of pregnant women (0.86±0.90). The frequency value in the group of newborns of T1DM mothers was 1.42 (±0.60), significantly (p<0.05) higher than in the corresponding control group (0.67±0.79). The value in the group of mothers with T1DM was significantly (p<0.05) higher than in their newborns. Comparing mothers without DM with their newborns, no significant frequency differences were observed. No significant correlations were observed between MN frequencies in mothers with T1DM and either the frequencies in their newborns, the duration of diabetes, or HbA1C levels. Our results indicate that T1DM is accompanied by increased frequencies of MN in pregnant women and their newborns.
authors.affiliation: Department of Biology and Medical Genetics, Medical University of Lodz, Pl. Hallera 1, 90-647 Lodz, Poland(4). Electronic address: monika.witczak@umed.lodz.pl.
authors.forename: Monika
authors.initials: M
authors.lastname: Witczak
authors.affiliation: Department of Biology and Medical Genetics, Medical University of Lodz, Pl. Hallera 1, 90-647 Lodz, Poland(4). Electronic address: tomfer@poczta.onet.pl.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Ferenc
authors.affiliation: Department of Neonatology, Polish Mother's Memorial Hospital - Research Institute, ul. Rzgowska 281/289, 93-338 Lodz, Poland(5). Electronic address: ewagulcz@wp.pl.
authors.forename: Ewa
authors.initials: E
authors.lastname: Gulczyńska
authors.affiliation: Feto-Maternal and Gynecology Department, Polish Mother's Memorial Hospital - Research Institute, Rzgowska 281/289, 93-338 Lodz, Poland(6). Electronic address: dorota.nowakowska@umed.lodz.pl.
authors.forename: Dorota
authors.initials: D
authors.lastname: Nowakowska
authors.affiliation: Department of Biology and Medical Genetics, Medical University of Lodz, Pl. Hallera 1, 90-647 Lodz, Poland(4). Electronic address: dobroslawa.lopaczynska@umed.lodz.pl.
authors.forename: Dobrosława
authors.initials: D
authors.lastname: Łopaczyńska
authors.affiliation: Feto-Maternal and Gynecology Department, Polish Mother's Memorial Hospital - Research Institute, Rzgowska 281/289, 93-338 Lodz, Poland(6). Electronic address: jan.wilczynski@umed.lodz.pl.
authors.forename: Jan
authors.initials: J
authors.lastname: Wilczyński
mesh_terms: D016022:Case-Control Studies; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics* / Q000378:metabolism; D005260:Female; D006801:Humans; D007231:Infant, Newborn / Q000097:blood; D008214:Lymphocytes / Q000473:pathology; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced* / Q000196:embryology* / Q000706:statistics & numerical data; D015162:Micronucleus Tests; D018384:Oxidative Stress / Q000235:genetics; D011247:Pregnancy; D011254:Pregnancy in Diabetics / Q000235:genetics* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species
keywords: Genotoxicity; Micronuclei; Newborns; Pregnancy; Type 1 diabetes mellitus