title: Genomic and phylogenetic characterization of viruses included in the Manzanilla and Oropouche species complexes of the genus Orthobunyavirus, family Bunyaviridae.
abstract: A thorough characterization of the genetic diversity of viruses present in vector and vertebrate host populations is essential for the early detection of and response to emerging pathogenic viruses, yet genetic characterization of many important viral groups remains incomplete. The Simbu serogroup of the genus Orthobunyavirus, family Bunyaviridae, is an example. The Simbu serogroup currently consists of a highly diverse group of related arboviruses that infect both humans and economically important livestock species. Here, we report complete genome sequences for 11 viruses within this group, with a focus on the large and poorly characterized Manzanilla and Oropouche species complexes. Phylogenetic and pairwise divergence analyses indicated the presence of high levels of genetic diversity within these two species complexes, on a par with that seen among the five other species complexes in the Simbu serogroup. Based on previously reported divergence thresholds between species, the data suggested that these two complexes should actually be divided into at least five species. Together these five species formed a distinct phylogenetic clade apart from the rest of the Simbu serogroup. Pairwise sequence divergences among viruses of this clade and viruses in other Simbu serogroup species complexes were similar to levels of divergence among the other orthobunyavirus serogroups. The genetic data also suggested relatively high levels of natural reassortment, with three potential reassortment events present, including two well-supported events involving viruses known to infect humans.
authors.affiliation: Center for Genomic Sciences, United States Army Medical Institute for Infectious Disease, Frederick, MD, USA.
authors.forename: Jason T
authors.initials: JT
authors.lastname: Ladner
authors.affiliation: Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA.
authors.forename: Nazir
authors.initials: N
authors.lastname: Savji
authors.affiliation: Center for Genomic Sciences, United States Army Medical Institute for Infectious Disease, Frederick, MD, USA.
authors.forename: Loreen
authors.initials: L
authors.lastname: Lofts
authors.affiliation: Center for Biodefense and Emerging Infectious Diseases, Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
authors.forename: Amelia
authors.initials: A
authors.lastname: Travassos da Rosa
authors.affiliation: Center for Genomic Sciences, United States Army Medical Institute for Infectious Disease, Frederick, MD, USA.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Wiley
authors.affiliation: Center for Genomic Sciences, United States Army Medical Institute for Infectious Disease, Frederick, MD, USA.
authors.forename: Marie C
authors.initials: MC
authors.lastname: Gestole
authors.affiliation: Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA.
authors.forename: Gail E
authors.initials: GE
authors.lastname: Rosen
authors.affiliation: Center for Biodefense and Emerging Infectious Diseases, Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
authors.forename: Hilda
authors.initials: H
authors.lastname: Guzman
authors.affiliation: Department of Arbovirology and Hemorrhagic Fevers, Instituto Evandro Chagas, Ananindeua, Brazil.
authors.forename: Pedro F C
authors.initials: PFC
authors.lastname: Vasconcelos
authors.affiliation: Virology Department, Naval Medical Research Unit Six, Lima, Peru.
authors.forename: Marcio R T
authors.initials: MRT
authors.lastname: Nunes
authors.affiliation: Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA.
authors.forename: Tadeusz
authors.initials: T
authors.lastname: J Kochel
authors.affiliation: Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA.
authors.forename: W Ian
authors.initials: WI
authors.lastname: Lipkin
authors.affiliation: Center for Biodefense and Emerging Infectious Diseases, Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
authors.forename: Robert B
authors.initials: RB
authors.lastname: Tesh
authors.affiliation: Center for Genomic Sciences, United States Army Medical Institute for Infectious Disease, Frederick, MD, USA.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Palacios
mesh_terms: D016000:Cluster Analysis; D014644:Genetic Variation; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016851:Orthobunyavirus / Q000145:classification* / Q000235:genetics*; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D012367:RNA, Viral