title: Analysis of L-serine-O-phosphate in cerebrospinal spinal fluid by derivatization-liquid chromatography/mass spectrometry.
abstract: L-serine-O-phosphate (L-SOP), the precursor of L-serine, is a potent agonist against the group III metabotropic glutamate receptors (mGluRs) and, thus, is of interest as a potential biomarker for monitoring modulation of neurotransmitter release. So far, no reports are available on the analysis of L-SOP in cerebrospinal fluid (CSF). Here a novel method is presented to determine L-SOP levels in CSF employing precolumn derivatization with (5-N-succinimidoxy-5-oxopentyl)triphenylphosphonium bromide (SPTPP) coupled to liquid chromatography/mass spectrometry (derivatization-LC/MS, d-LC/MS).
authors.affiliation: Discovery Analytical Sciences, Pharmaceutical Candidate Optimization, Research & Development, Bristol-Myers Squibb, Wallingford, CT 06492, USA.
authors.forename: Colleen A
authors.initials: CA
authors.lastname: McNaney
authors.affiliation: Bioanalytical Research, Pharmaceutical Candidate Optimization, Research & Development, Bristol-Myers Squibb, Wallingford, CT 06492, USA.
authors.forename: Yulia
authors.initials: Y
authors.lastname: Benitex
authors.affiliation: Exploratory Clinical and Translational Research, Research & Development, Bristol-Myers Squibb, Wallingford, CT 06492, USA.
authors.forename: David
authors.initials: D
authors.lastname: Luchetti
authors.affiliation: Exploratory Clinical and Translational Research, Research & Development, Bristol-Myers Squibb, Wallingford, CT 06492, USA.
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Labasi
authors.affiliation: Bioanalytical Research, Pharmaceutical Candidate Optimization, Research & Development, Bristol-Myers Squibb, Lawrenceville, NJ 08543, USA.
authors.forename: Timothy V
authors.initials: TV
authors.lastname: Olah
authors.affiliation: Bioanalytical Research, Pharmaceutical Candidate Optimization, Research & Development, Bristol-Myers Squibb, Wallingford, CT 06492, USA.
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Morgan
authors.affiliation: Discovery Analytical Sciences, Pharmaceutical Candidate Optimization, Research & Development, Bristol-Myers Squibb, Wallingford, CT 06492, USA. Electronic address: dieter.drexler@bms.com.
authors.forename: Dieter M
authors.initials: DM
authors.lastname: Drexler
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D009943:Organophosphorus Compounds / Q000737:chemistry; D010768:Phosphoserine / Q000134:cerebrospinal fluid* / Q000737:chemistry*; D013388:Succinimides / Q000737:chemistry
chemical_list: C550832:(5-N-succinimidoxy-5-oxopentyl)triphenylphosphonium; D009943:Organophosphorus Compounds; D013388:Succinimides; D010768:Phosphoserine
keywords: CSF; Derivatization; LC/MS; l-Serine-O-phosphate (l-SOP)