title: RNA-guided genome editing of mammalian cells.
abstract: The microbial CRISPR-Cas adaptive immune system can be harnessed to facilitate genome editing in eukaryotic cells (Cong L et al., Science 339, 819-823, 2013; Mali P et al., Science 339, 823-826, 2013). Here we describe a protocol for the use of the RNA-guided Cas9 nuclease from the Streptococcus pyogenes type II CRISPR system to achieve specific, scalable, and cost-efficient genome editing in mammalian cells.
authors.affiliation: Broad Institute of MIT and Harvard, Massachusetts Institute of Technology, Cambridge, USA.
authors.forename: Neena K
authors.initials: NK
authors.lastname: Pyzocha
authors.forename: F Ann
authors.initials: FA
authors.lastname: Ran
authors.forename: Patrick D
authors.initials: PD
authors.lastname: Hsu
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D059766:DNA End-Joining Repair; D023061:Gene Order; D018390:Gene Targeting / Q000379:methods; D005822:Genetic Vectors; D016678:Genome; D006801:Humans; D017393:RNA Editing; D014162:Transfection; D058727:RNA, Small Untranslated
chemical_list: D058727:RNA, Small Untranslated