title: Micro-patterning of mammalian cells on suspended MEMS resonant sensors for long-term growth measurements.
abstract: MEMS resonant mass sensors can measure the mass of individual cells, though long-term growth measurements are limited by the movement of cells off the sensor area. Micro-patterning techniques are a powerful approach to control the placement of individual cells in an arrayed format. In this work we present a method for micro-patterning cells on fully suspended resonant sensors through select functionalization and passivation of the chip surface. This method combines high-resolution photolithography with a blanket transfer technique for applying photoresist to avoid damaging the sensors. Cells are constrained to the patterned collagen area on the sensor by pluronic acting as a cell adhesion blocker. This micro-patterning method enables long-term growth measurements, which is demonstrated by a measurement of the change in mass of a human breast cancer cell over 18 h.
authors.affiliation: Department of Mechanical Science and Engineering, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA.
authors.forename: Elise A
authors.initials: EA
authors.lastname: Corbin
authors.forename: Brian R
authors.initials: BR
authors.lastname: Dorvel
authors.forename: Larry J
authors.initials: LJ
authors.lastname: Millet
authors.forename: William P
authors.initials: WP
authors.lastname: King
authors.forename: Rashid
authors.initials: R
authors.lastname: Bashir
mesh_terms: D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D055616:Microtechnology / Q000295:instrumentation*; D013499:Surface Properties