title: Genetic association of CHEK2, GSTP1, and ERCC1 with glioblastoma in the Han Chinese population.
abstract: Glioblastoma (GBM), a deadly brain tumor, is the most malignant glioma. It mainly occurs in adults and occurs significantly more in males than in females. We genotyped 19 tag single nucleotide polymorphisms (tSNPs) from 13 genes in a case-control study of the Han Chinese population to identify genetic factors contributing to the risk of GBM. These tSNPs were genotyped by Sequenom MassARRAY RS1000. Statistical analysis was performed using χ(2) test and SNPStats, a website software. Using χ(2) test, we found that the distribution of two tSNPs (rs2267130 in checkpoint kinase 2 (CHEK2), p = 0.040; rs1695 in GSTP1, p = 0.023) allelic frequencies had significant difference between cases and controls. When we analyzed all of the tSNPs using the SNPStats software, we found that rs1695 in GSTP1 decreased the risk of GBM in log-additive model (OR = 0.56, 95% CI, 0.34-0.94, p = 0.022). Besides, we found that there is an interaction between rs3212986 in excision repair cross-complementing group 1 (ERCC1) and gender under codominant and recessive models. The gene polymorphisms in CHEK2, GSTP1, and ERCC1 may be involved in GBM in the Han Chinese population. Since our sample size is small, further investigation needs to be performed.
authors.affiliation: Department of Neurosurgery, Tangdu Hospital, The Fourth Military Medical University, No. 1, Xinsi Road, Xi'an, 710038, China.
authors.forename: Yu-Shu
authors.initials: YS
authors.lastname: Dong
authors.forename: Wu-Gang
authors.initials: WG
authors.lastname: Hou
authors.forename: Xiao-Lan
authors.initials: XL
authors.lastname: Li
authors.forename: Tian-Bo
authors.initials: TB
authors.lastname: Jin
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.forename: Da-Yun
authors.initials: DY
authors.lastname: Feng
authors.forename: De-Bao
authors.initials: DB
authors.lastname: Liu
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: Gao
authors.forename: Zhong-Min
authors.initials: ZM
authors.lastname: Yin
authors.forename: Huai-Zhou
authors.initials: HZ
authors.lastname: Qin
mesh_terms: D001932:Brain Neoplasms / Q000235:genetics*; D016022:Case-Control Studies; D064447:Checkpoint Kinase 2 / Q000235:genetics*; D002681:China / Q000208:ethnology; D004268:DNA-Binding Proteins / Q000235:genetics*; D004720:Endonucleases / Q000235:genetics*; D005260:Female; D005909:Glioblastoma / Q000235:genetics*; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D017382:Reactive Oxygen Species; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi; D064447:Checkpoint Kinase 2; C578634:CHEK2 protein, human; C071447:ERCC1 protein, human; D004720:Endonucleases