title: Chronic administration of nicotine enhances NMDA-activated currents in the prefrontal cortex and core part of the nucleus accumbens of rats.
abstract: Nicotine is an addictive substance of tobacco. It has been suggested that nicotine acts on glutamatergic (N-methyl-d-aspartate, NMDA) neurotransmission affecting dopamine release in the mesocorticolimbic system. This effect is reflected in neuroadaptative changes that can modulate neurotransmission in the prefrontal cortex (PFC) and nucleus accumbens (NAcc) core (cNAcc) and shell (sNAcc) regions. We evaluated the effect of chronic administration of nicotine (4.23 mg/kg/day for 14 days) on NMDA activated currents in dissociated neurons from the PFC, and NAcc (from core and shell regions). We assessed nicotine blood levels by mass spectrophotometry and we confirmed that nicotine increases locomotor activity. An electrophysiological study showed an increase in NMDA currents in neurons from the PFC and core part of the NAcc in animals treated with nicotine compared to those of control rats. No change was observed in neurons from the shell part of the NAcc. The enhanced glutamatergic activity observed in the neurons of rats with chronic administration of nicotine may explain the increased locomotive activity also observed in such rats. To assess one of the possible causes of increased NMDA currents, we used magnesium, to block NMDA receptor that contains the NR2B subunit. If there is a change in percent block of NMDA currents, it means that there is a possible change in expression of NMDA receptor subunits. Our results showed that there is no difference in the blocking effect of magnesium on the NMDA currents. The magnesium lacks of effect after nicotinic treatment suggests that there is no change in expression of NR2B subunit of NMDA receptors, then, the effect of nicotine treatment on amplitude of NMDA currents may be due to an increase in the quantity of receptors or to a change in the unitary conductance, rather than a change in the expression of the subunits that constitute it.
authors.affiliation: Instituto de Fisiología, Benemérita Universidad Autónoma de Puebla, 14 Sur 6301, CP 72570, Puebla, Puebla, México.
authors.forename: Tania
authors.initials: T
authors.lastname: Ávila-Ruiz
authors.forename: Vladimir
authors.initials: V
authors.lastname: Carranza
authors.forename: López-López
authors.initials: LL
authors.lastname: Gustavo
authors.forename: Daniel I
authors.initials: DI
authors.lastname: Limón
authors.forename: Isabel
authors.initials: I
authors.lastname: Martínez
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Flores
authors.forename: Jorge
authors.initials: J
authors.lastname: Flores-Hernández
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002478:Cells, Cultured; D005731:Ganglionic Stimulants / Q000008:administration & dosage* / Q000097:blood; D066298:In Vitro Techniques; D017616:Magnesium Compounds / Q000494:pharmacology; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D009043:Motor Activity / Q000187:drug effects; D016202:N-Methylaspartate / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D018377:Neurotransmitter Agents / Q000494:pharmacology; D009538:Nicotine / Q000008:administration & dosage* / Q000097:blood; D009714:Nucleus Accumbens / Q000187:drug effects* / Q000502:physiology; D018408:Patch-Clamp Techniques; D017397:Prefrontal Cortex / Q000187:drug effects* / Q000502:physiology; D017966:Pyramidal Cells / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D005731:Ganglionic Stimulants; D017616:Magnesium Compounds; C121001:NR2B NMDA receptor; D018377:Neurotransmitter Agents; D016194:Receptors, N-Methyl-D-Aspartate; D016202:N-Methylaspartate; D009538:Nicotine
keywords: NMDA; locomotive activity; nicotine; nucleus accumbens; prefrontal cortex