title: Interaction of citrate-coated silver nanoparticles with earthworm coelomic fluid and related cytotoxicity in Eisenia andrei.
abstract: Understanding the interaction of nanoparticles with biological fluid is important for predicting the behavior and toxicity of nanoparticles in living systems. The earthworm Eisenia andrei was exposed to citrate-coated silver nanoparticles (cAgNPs), and the interaction of cAgNPs with earthworm coelomic fluid (ECF), the cytotoxicity of cAgNPs in earthworm coelomocytes was assessed. The neutral red retention assay showed a reduction in lysosomal stability after exposure. The toxicity of silver ions dissolved from cAgNPs in the soil medium was not significant. The aggregation and dissolution of cAgNPs increased in ECF, which contains various electrolytes that alter the properties of nanoparticles, and their subsequent toxicity. Microscopic and dissolution studies demonstrated that the aggregation of cAgNPs rapidly increased, and readily dissolved in ECF. The bioavailability of cAgNPs to earthworms induced lysosomal cytotoxicity. This is the first report to test the interaction and lysosomal cytotoxicity of nanoparticles in earthworm biofluids.
authors.affiliation: Department of Environmental Health Science, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Korea.
authors.forename: Jin Il
authors.initials: JI
authors.lastname: Kwak
authors.forename: Woo-Mi
authors.initials: WM
authors.lastname: Lee
authors.forename: Shin Woong
authors.initials: SW
authors.lastname: Kim
authors.forename: Youn-Joo
authors.initials: YJ
authors.lastname: An
mesh_terms: D000818:Animals; D001682:Biological Availability; D001826:Body Fluids / Q000737:chemistry*; D002951:Citrates / Q000737:chemistry* / Q000493:pharmacokinetics; D008247:Lysosomes / Q000187:drug effects*; D053768:Metal Nanoparticles / Q000633:toxicity*; D009499:Neutral Red; D009835:Oligochaeta / Q000187:drug effects*; D012834:Silver / Q000493:pharmacokinetics / Q000633:toxicity*; D012987:Soil / Q000737:chemistry; D012989:Soil Pollutants / Q000633:toxicity
chemical_list: D002951:Citrates; D012987:Soil; D012989:Soil Pollutants; D009499:Neutral Red; D012834:Silver
keywords: Eisenia andrei, biofluid; cytotoxicity; earthworm coelomic fluid; silver nanoparticle