title: Influence of particle geometry and PEGylation on phagocytosis of particulate carriers.
abstract: Particle geometry of micro- and nanoparticles has been identified as an important design parameter to influence the interaction with cells such as macrophages. A head to head comparison of elongated, non-spherical and spherical micro- and nanoparticles with and without PEGylation was carried out to benchmark two phagocytosis inhibiting techniques. J774.A1 macrophages were incubated with fluorescently labeled PLGA micro- and nanoparticles and analyzed by confocal laser scanning microscope (CLSM) and flow cytometry (FACS). Particle uptake into macrophages was significantly reduced upon PEGylation or elongated particle geometry. A combination of both, an elongated shape and PEGylation, had the strongest phagocytosis inhibiting effect for nanoparticles.
authors.affiliation: Department of Pharmacy, Pharmaceutical Technology & Biopharmaceutics, Ludwig-Maximillians-University Munich, Butenandtstr. 5-8 D-81377 Munich, Germany.
authors.forename: Roman
authors.initials: R
authors.lastname: Mathaes
authors.affiliation: Department of Pharmacy, Pharmaceutical Technology & Biopharmaceutics, Ludwig-Maximillians-University Munich, Butenandtstr. 5-8 D-81377 Munich, Germany.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Winter
authors.affiliation: Department of Pharmacy, Pharmaceutical Technology & Biopharmaceutics, Ludwig-Maximillians-University Munich, Butenandtstr. 5-8 D-81377 Munich, Germany. Electronic address: ahmed.besheer@cup.uni-muenchen.de.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Besheer
authors.affiliation: Department of Pharmacy, Pharmaceutical Technology & Biopharmaceutics, Ludwig-Maximillians-University Munich, Butenandtstr. 5-8 D-81377 Munich, Germany. Electronic address: julia.engert@cup.uni-muenchen.de.
authors.forename: Julia
authors.initials: J
authors.lastname: Engert
mesh_terms: D000818:Animals; D002460:Cell Line; D004337:Drug Carriers; D005434:Flow Cytometry; D019344:Lactic Acid / Q000737:chemistry / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D018613:Microscopy, Confocal; D053758:Nanoparticles; D010316:Particle Size; D010587:Phagocytosis; D011092:Polyethylene Glycols / Q000737:chemistry / Q000378:metabolism*; D011100:Polyglycolic Acid / Q000737:chemistry / Q000378:metabolism*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013499:Surface Properties
chemical_list: D004337:Drug Carriers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011092:Polyethylene Glycols
keywords: Flow cytometry; Geometry; Macrophages; Non-spherical particles; PEGylation; Phagocytosis