title: Micropatterning cells on permeable membrane filters.
abstract: Epithelium is abundantly present in the human body as it lines most major organs. Therefore, ensuring the proper function of epithelium is pivotal for successfully engineering whole organ replacements. An important characteristic of mature epithelium is apical-basal polarization which can be obtained using the air-liquid interface (ALI) culture system. Micropatterning is a widely used bioengineering strategy to spatially control the location and organization of cells on tissue culture substrates. Micropatterning is therefore an interesting method for generating patterned epithelium. Enabling micropatterning of epithelial cells however requires micropatterning methods that are designed to (i) be compatible with permeable membranes substrates and (ii) allow prolonged culture of patterned cells, both of which are required for appropriate epithelial apical-basal polarization. Here, we describe a number of methods we have developed for generating monoculture as well as coculture of epithelial cells that are compatible with ALI culture.
authors.affiliation: Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada.
authors.forename: Sahar
authors.initials: S
authors.lastname: Javaherian
authors.affiliation: Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada; Institute for Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada.
authors.forename: Ana C
authors.initials: AC
authors.lastname: Paz
authors.affiliation: Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada; Institute for Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada.
authors.forename: Alison P
authors.initials: AP
authors.lastname: McGuigan
mesh_terms: D000818:Animals; D057005:Bioengineering; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D002463:Cell Membrane Permeability; D016764:Cell Polarity / Q000502:physiology; D018920:Coculture Techniques / Q000379:methods; D004285:Dogs; D004847:Epithelial Cells / Q000502:physiology*; D004848:Epithelium / Q000502:physiology; D006801:Humans; D061985:Madin Darby Canine Kidney Cells / Q000502:physiology; D008567:Membranes, Artificial; D008851:Micropore Filters; D013499:Surface Properties
chemical_list: D008567:Membranes, Artificial
keywords: Air–liquid interface culture; Apical–basal polarization; Epithelial cells; Filter insert membrane; Micropatterning; Soft lithography