title: Binding of von Willebrand factor and plasma proteins to the eggshell of Schistosoma mansoni.
abstract: Schistosoma mansoni eggs have to cross the endothelium and intestinal wall to leave the host and continue the life cycle. Mechanisms involved in this essential step are largely unknown. Here we describe direct binding to the S. mansoni eggshell of von Willebrand factor and other plasma proteins involved in haemostasis. Using deletion-mutants, we demonstrated that it is the A1 domain of von Willebrand factor that binds to the eggshell. Our results suggest that binding of plasma proteins to the eggshell promotes binding to the endothelium, initiating the passage of the egg through the blood-vessel wall to be excreted in the end.
authors.affiliation: Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center, Rotterdam, The Netherlands.
authors.forename: Saskia
authors.initials: S
authors.lastname: Dewalick
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Hensbergen
authors.affiliation: Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center, Rotterdam, The Netherlands.
authors.forename: Michiel L
authors.initials: ML
authors.lastname: Bexkens
authors.affiliation: Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center, Rotterdam, The Netherlands.
authors.forename: Christina
authors.initials: C
authors.lastname: Grosserichter-Wagener
authors.affiliation: Department of Parasitology, Center for Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Cornelis H
authors.initials: CH
authors.lastname: Hokke
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: André M
authors.initials: AM
authors.lastname: Deelder
authors.affiliation: Department of Clinical Chemistry and Haematology, University Medical Center Utrecht, The Netherlands.
authors.forename: Philip G
authors.initials: PG
authors.lastname: de Groot
authors.affiliation: Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center, Rotterdam, The Netherlands.
authors.forename: Aloysius G M
authors.initials: AG
authors.lastname: Tielens
authors.affiliation: Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center, Rotterdam, The Netherlands. Electronic address: j.vanhellemond@erasmusmc.nl.
authors.forename: Jaap J
authors.initials: JJ
authors.lastname: van Hellemond
mesh_terms: D000328:Adult; D000818:Animals; D001665:Binding Sites; D001798:Blood Proteins / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding; D012550:Schistosoma mansoni / Q000254:growth & development / Q000378:metabolism*; D017384:Sequence Deletion; D015053:Zygote / Q000378:metabolism*; D014841:von Willebrand Factor / Q000235:genetics / Q000378:metabolism*
chemical_list: D001798:Blood Proteins; D014841:von Willebrand Factor
keywords: Egg excretion; Eggshell; Haemostasis; Plasma proteins; Schistosoma mansoni; Schistosomiasis; von Willebrand factor