title: UVB-induced gene expression in the skin of Xiphophorus maculatus Jp 163 B.
abstract: Xiphophorus fish and interspecies hybrids represent long-standing models to study the genetics underlying spontaneous and induced tumorigenesis. The recent release of the Xiphophorus maculatus genome sequence will allow global genetic regulation studies of genes involved in the inherited susceptibility to UVB-induced melanoma within select backcross hybrids. As a first step toward this goal, we report results of an RNA-Seq approach to identify genes and pathways showing modulated transcription within the skin of X. maculatus Jp 163 B upon UVB exposure. X. maculatus Jp 163 B were exposed to various doses of UVB followed by RNA-Seq analysis at each dose to investigate overall gene expression in each sample. A total of 357 genes with a minimum expression change of 4-fold (p-adj<0.05) were identified as responsive to UVB. The molecular genetic response of Xiphophorus skin to UVB exposure permitted assessment of; (1) the basal expression level of each transcript for each skin sample, (2) the changes in expression levels for each gene in the transcriptome upon exposure to increasing doses of UVB, and (3) clusters of genes that exhibit similar patterns of change in expression upon UVB exposure. These data provide a foundation for understanding the molecular genetic response of fish skin to UVB exposure.
authors.affiliation: Molecular Biosciences Research Group, Department of Chemistry and Biochemistry, Texas State University, San Marcos, TX, United States. Electronic address: k_y18@txstate.edu.
authors.forename: Kuan
authors.initials: K
authors.lastname: Yang
authors.affiliation: Molecular Biosciences Research Group, Department of Chemistry and Biochemistry, Texas State University, San Marcos, TX, United States. Electronic address: mboswell@txstate.edu.
authors.forename: Mikki
authors.initials: M
authors.lastname: Boswell
authors.affiliation: Molecular Biosciences Research Group, Department of Chemistry and Biochemistry, Texas State University, San Marcos, TX, United States. Electronic address: djw3@txstate.edu.
authors.forename: Dylan J
authors.initials: DJ
authors.lastname: Walter
authors.affiliation: Molecular Biosciences Research Group, Department of Chemistry and Biochemistry, Texas State University, San Marcos, TX, United States. Electronic address: kd1186@txstate.edu.
authors.forename: Kevin P
authors.initials: KP
authors.lastname: Downs
authors.affiliation: Molecular Biosciences Research Group, Department of Chemistry and Biochemistry, Texas State University, San Marcos, TX, United States. Electronic address: kg1217@txstate.edu.
authors.forename: Kimberly
authors.initials: K
authors.lastname: Gaston-Pravia
authors.affiliation: Molecular Biosciences Research Group, Department of Chemistry and Biochemistry, Texas State University, San Marcos, TX, United States. Electronic address: tg23@txstate.edu.
authors.forename: Tzintzuni
authors.initials: T
authors.lastname: Garcia
authors.affiliation: Molecular Biosciences Research Group, Department of Chemistry and Biochemistry, Texas State University, San Marcos, TX, United States. Electronic address: ys14@txstate.edu.
authors.forename: Yingjia
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Carcinogenesis, University of Texas MD Anderson Cancer Center, Smithville, TX, United States. Electronic address: dmitchel@mdanderson.org.
authors.forename: David L
authors.initials: DL
authors.lastname: Mitchell
authors.affiliation: Molecular Biosciences Research Group, Department of Chemistry and Biochemistry, Texas State University, San Marcos, TX, United States. Electronic address: RWalter@txstate.edu.
authors.forename: Ronald B
authors.initials: RB
authors.lastname: Walter
mesh_terms: D000818:Animals; D001483:Base Sequence; D003532:Cyprinodontiformes / Q000235:genetics*; D015870:Gene Expression / Q000528:radiation effects*; D008297:Male; D008969:Molecular Sequence Data; D017423:Sequence Analysis, RNA; D012867:Skin / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays / Q000009:adverse effects*
keywords: Fishes; Gene expression; RNA-Seq; Transcriptome; UVB; Ultraviolet light; Xiphophorus