title: Detection of Giardia duodenalis assemblage A and B isolates by immunochromatography in stool samples from Rwandan children.
abstract: We evaluated the performance of an immunochromatographic assay (ICA) in comparison with light microscopy and PCR for the detection of Giardia duodenalis in stool samples from 558 Rwandan children. The association of infection with clinical symptoms was similar for the three diagnostic tools. The ICA equally detected parasites of assemblages A and B and was more sensitive than light microscopy (50.4 versus 29.5% of PCR-positive samples considered true positive; p <0.0001). Hence, the ICA shows superior sensitivity compared with microscopy but still misses half of the G. duodenalis infections detected by PCR in this hyperendemic area.
authors.affiliation: Institute of Tropical Medicine and International Health, Charité - Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Ignatius
authors.forename: J B
authors.initials: JB
authors.lastname: Gahutu
authors.forename: C
authors.initials: C
authors.lastname: Klotz
authors.forename: A
authors.initials: A
authors.lastname: Musemakweri
authors.forename: T
authors.initials: T
authors.lastname: Aebischer
authors.forename: F P
authors.initials: FP
authors.lastname: Mockenhaupt
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D005243:Feces / Q000469:parasitology; D016829:Giardia lamblia / Q000145:classification* / Q000302:isolation & purification*; D005873:Giardiasis / Q000175:diagnosis* / Q000453:epidemiology; D006801:Humans; D008853:Microscopy; D016133:Polymerase Chain Reaction; D012432:Rwanda / Q000453:epidemiology; D012680:Sensitivity and Specificity
keywords: Genotypes; Giardia duodenalis; PCR; Rwanda; immunochromatography; microscopy