title: Interleukin-17A and -17F genes polymorphisms in lung cancer.
abstract: Interleukin-17 (IL-17) is a proinflammatory cytokines produced by T helper 17 (Th17) cells, which plays an important role in both innate and adaptive immune systems. Genetic variants in the IL-17 genes may influence the immunopathogenesis of many cancers. In our study, we investigated the association of three single-nucleotide polymorphisms (SNPs: -152 G/A, 7488 A/G and 7383 A/G) in the IL-17A and IL-17F genes with lung cancer risk, in the Tunisian population. The genotypic and allelic distributions of IL-17A and IL-17F genes polymorphisms were analyzed by Polymerase Chain-Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) for 239 patients and 258 healthy controls. Our results revealed a statistically significant association between IL-17F 7488G allele and increased lung cancer risk (P=0.028). Stratification analysis indicated that IL-17F 7488G allele enhances the risk of lung cancer development among men and oldest age subject groups (P<0.05). Patients with IL-17F 7488G allele were also more likely to be diagnosed at advanced stage (P=0.04), or with metastatic lung cancer (P=0.035). Furthermore, no significant association between IL-17F 7383 A/G, IL-17 -152G/A polymorphisms and lung cancer risk was observed (P>0.05). However, we reported contradictory findings on the association of IL-17 -152 G/A polymorphisms with lung cancer risk. In addition, we suggested the existence of a biological interaction between IL-17A, but not IL-17F polymorphisms and smoking. Our findings suggest that IL-17F 7488G allele is associated with increased lung cancer risk in the Tunisian population.
authors.affiliation: Tunis El Manar University, Medicine School of Tunis, Department of Basic Sciences, Tunisia; A. Mami Hospital of Pneumology, Unit Research UR/12-SP-15, Homeostasis and Cell Dysfunction, Ariana, Tunisia. Electronic address: Kaabachi.wajih@gmail.com.
authors.forename: Wajih
authors.initials: W
authors.lastname: Kaabachi
authors.affiliation: Tunis El Manar University, Medicine School of Tunis, Department of Basic Sciences, Tunisia; A. Mami Hospital of Pneumology, Unit Research UR/12-SP-15, Homeostasis and Cell Dysfunction, Ariana, Tunisia. Electronic address: amourabenmor@gmail.fr.
authors.forename: Amira
authors.initials: A
authors.lastname: ben Amor
authors.affiliation: Tunis El Manar University, Medicine School of Tunis, Department of Basic Sciences, Tunisia; A. Mami Hospital of Pneumology, Unit Research UR/12-SP-15, Homeostasis and Cell Dysfunction, Ariana, Tunisia. Electronic address: Kaabachi.safa@yahoo.com.
authors.forename: Safa
authors.initials: S
authors.lastname: Kaabachi
authors.affiliation: Tunis El Manar University, Medicine School of Tunis, Department of Basic Sciences, Tunisia; A. Mami Hospital of Pneumology, Unit Research UR/12-SP-15, Homeostasis and Cell Dysfunction, Ariana, Tunisia. Electronic address: rafa.ahem@yahoo.fr.
authors.forename: Ahlem
authors.initials: A
authors.lastname: Rafrafi
authors.affiliation: Tunis El Manar University, Medicine School of Tunis, Department of Basic Sciences, Tunisia; A. Mami Hospital of Pneumology, Unit Research UR/12-SP-15, Homeostasis and Cell Dysfunction, Ariana, Tunisia. Electronic address: kalthoumzaoui@yahoo.fr.
authors.forename: Kalthoum
authors.initials: K
authors.lastname: Tizaoui
authors.affiliation: Tunis El Manar University, Medicine School of Tunis, Department of Basic Sciences, Tunisia; A. Mami Hospital of Pneumology, Unit Research UR/12-SP-15, Homeostasis and Cell Dysfunction, Ariana, Tunisia. Electronic address: kamel.hamzaoui@gmail.com.
authors.forename: Kamel
authors.initials: K
authors.lastname: Hamzaoui
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D059647:Gene-Environment Interaction; D020022:Genetic Predisposition to Disease; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D020381:Interleukin-17 / Q000235:genetics*; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012307:Risk Factors; D012907:Smoking / Q000235:genetics
chemical_list: D020381:Interleukin-17
keywords: Biological interaction; Interleukin-17; Lung cancer; Polymorphisms; Restriction fragment length polymorphism