title: Homo-fermentative production of D-lactic acid by Lactobacillus sp. employing casein whey permeate as a raw feed-stock.
abstract: Casein whey permeate (CWP), a lactose-enriched dairy waste effluent, is a viable feed stock for the production of value-added products. Two lactic acid bacteria were cultivated in a synthetic casein whey permeate medium with or without pH control. Lactobacillus lactis ATCC 4797 produced D-lactic acid (DLA) at 12.5 g l(-1) in a bioreactor. The values of Leudking-Piret model parameters suggested that lactate was a growth-associated product. Batch fermentation was also performed employing CWP (35 g lactose l(-1)) with casein hydrolysate as a nitrogen supplement in a bioreactor. After 40 h, L. lactis produced 24.3 g lactic acid l(-1) with an optical purity >98 %. Thus CWP may be regarded as a potential feed-stock for DLA production.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Guwahati, Guwahati, Assam, 781039, India, saurav.prasad@iitg.ernet.in.
authors.forename: Saurav
authors.initials: S
authors.lastname: Prasad
authors.forename: Katla
authors.initials: K
authors.lastname: Srikanth
authors.forename: Anil M
authors.initials: AM
authors.lastname: Limaye
authors.forename: Senthilkumar
authors.initials: S
authors.lastname: Sivaprakasam
mesh_terms: D019149:Bioreactors; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D005511:Food Handling; D007220:Industrial Waste; D019344:Lactic Acid / Q000378:metabolism*; D007778:Lactobacillus / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D003470:Culture Media; D007220:Industrial Waste; D019344:Lactic Acid