title: NMDA receptor-dependent multidendrite Ca(2+) spikes required for hippocampal burst firing in vivo.
abstract: High-frequency bursts of action potentials (APs) are a distinctive form of signaling in various types of mammalian central neurons. In CA1 hippocampal pyramidal neurons in vivo, such complex spike bursts (CSs) are detected during various behaviors and are considered to be particularly important for learning- and memory-related synaptic plasticity. Here, we combined whole-cell recordings and two-photon imaging in mouse CA1 pyramidal neurons to investigate the cellular mechanisms underlying CSs in vivo. Our results demonstrate that CSs are of synaptic origin, as they require N-methyl-D-aspartate (NMDA) receptor activation. We identify voltage-gated Ca(2+) channel-dependent, spike-like depolarizations as integral components of the CSs. These Ca(2+) spikes were invariably associated with widespread large-amplitude Ca(2+) transients in basal and apical dendrites. Together, our results reveal a type of NMDA receptor-dependent multidendrite Ca(2+) spike required for high-frequency bursting in vivo.
authors.affiliation: Institute of Neuroscience, Center for Integrated Protein Science and SyNergy Cluster, Technical University Munich, Biedersteiner Straße 29, Munich 80802, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Grienberger
authors.affiliation: Institute of Neuroscience, Center for Integrated Protein Science and SyNergy Cluster, Technical University Munich, Biedersteiner Straße 29, Munich 80802, Germany; Brain Research Center, Third Military Medical University, Chongqing 400038, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Chen
authors.affiliation: Institute of Neuroscience, Center for Integrated Protein Science and SyNergy Cluster, Technical University Munich, Biedersteiner Straße 29, Munich 80802, Germany. Electronic address: arthur.konnerth@lrz.tum.de.
authors.forename: Arthur
authors.initials: A
authors.lastname: Konnerth
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003712:Dendrites / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D018408:Patch-Clamp Techniques / Q000379:methods; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D013569:Synapses / Q000378:metabolism
chemical_list: D016194:Receptors, N-Methyl-D-Aspartate; D002118:Calcium