title: Residual gas analyzer mass spectrometry for human breath analysis: a new tool for the non-invasive diagnosis of Helicobacter pylori infection.
abstract: A residual gas analyzer (RGA) coupled with a high vacuum chamber is described for the non-invasive diagnosis of the Helicobacter pylori (H. pylori) infection through ¹³C-urea breath analysis. The present RGA-based mass spectrometry (MS) method is capable of measuring high-precision ¹³CO₂ isotope enrichments in exhaled breath samples from individuals harboring the H. pylori infection. The system exhibited 100% diagnostic sensitivity, and 93% specificity alongside positive and negative predictive values of 95% and 100%, respectively, compared with invasive endoscopy-based biopsy tests. A statistically sound diagnostic cut-off value for the presence of H. pylori was determined to be 3.0‰ using a receiver operating characteristic curve analysis. The diagnostic accuracy and validity of the results are also supported by optical off-axis integrated cavity output spectroscopy measurements. The δ¹³(DOB)C‰ values of both methods correlated well (R² = 0.9973 at 30 min). The RGA-based instrumental setup described here is simple, robust, easy-to-use and more portable and cost-effective compared to all other currently available detection methods, thus making it a new point-of-care medical diagnostic tool for the purpose of large-scale screening of the H. pylori infection in real time. The RGA-MS technique should have broad applicability for ¹³C-breath tests in a wide range of biomedical research and clinical diagnostics for many other diseases and metabolic disorders.
authors.affiliation: Department of Chemical, Biological and Macromolecular Sciences, S N Bose National Centre for Basic Sciences, Salt Lake, JD Block, Sector III, Kolkata 700098, India.
authors.forename: Abhijit
authors.initials: A
authors.lastname: Maity
authors.forename: Gourab D
authors.initials: GD
authors.lastname: Banik
authors.forename: Chiranjit
authors.initials: C
authors.lastname: Ghosh
authors.forename: Suman
authors.initials: S
authors.lastname: Som
authors.forename: Sujit
authors.initials: S
authors.lastname: Chaudhuri
authors.forename: Sunil B
authors.initials: SB
authors.lastname: Daschakraborty
authors.forename: Shibendu
authors.initials: S
authors.lastname: Ghosh
authors.forename: Barnali
authors.initials: B
authors.lastname: Ghosh
authors.forename: Arup K
authors.initials: AK
authors.lastname: Raychaudhuri
authors.forename: Manik
authors.initials: M
authors.lastname: Pradhan
mesh_terms: D000328:Adult; D000368:Aged; D001944:Breath Tests / Q000295:instrumentation* / Q000379:methods*; D002245:Carbon Dioxide / Q000378:metabolism; D002247:Carbon Isotopes; D045853:Exhalation; D005260:Female; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000378:metabolism; D016480:Helicobacter pylori / Q000502:physiology*; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000295:instrumentation*; D008875:Middle Aged; D012372:ROC Curve; D012044:Regression Analysis; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis; D014508:Urea / Q000032:analysis; D055815:Young Adult
chemical_list: D002247:Carbon Isotopes; D002245:Carbon Dioxide; D014508:Urea