title: Eukaryotic expression systems for structural studies.
abstract: This protocol describes protein production in mammalian cells by transient transfection. It assumes the expression construct contains either a 6-HIS or Fc fusion tag to allow recovery of the protein by affinity chromatography. The method is one of the simplest available for protein expression in eukaryotic cells, requires little specialized equipment, and has a reasonably high rate of success.
authors.affiliation: Center for Structural Genomics of Infectious Diseases, Computational Institute, University of Chicago, Chicago, IL, 60557, USA.
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Nelson
authors.forename: William H
authors.initials: WH
authors.lastname: McCoy
authors.forename: Daved H
authors.initials: DH
authors.lastname: Fremont
mesh_terms: D000818:Animals; D019295:Computational Biology; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008967:Molecular Biology / Q000379:methods*; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D040901:Proteomics / Q000379:methods; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D059467:Transcriptome
chemical_list: D011506:Proteins; D011993:Recombinant Fusion Proteins