title: Influence of daily immersion in denture cleanser on multispecies biofilm.
abstract: OBJECTIVE
  The aim of this study was to evaluate the effect of daily exposure to a denture cleanser on a multispecies biofilm.
  
  
  METHODS
  Multispecies biofilms (five bacteria and Candida albicans) were developed for 64.5 h on acrylic resin specimens and randomized into control and experimental groups. In the experimental group, biofilms were immersed in denture cleanser for 3 min/day for seven consecutive days. In the control group, the biofilms were developed with no treatment for the same period. Biofilms from both groups were collected after 1, 4, and 7 days and analyzed for the number of microorganisms and polysaccharide concentrations. Scanning electron microscopy (SEM) and confocal microscopy (CLSM) analyses were performed.
  
  
  RESULTS
  The total microorganism counts and bacterial populations were lower in the experimental group compared to the control group for all of the periods evaluated. However, the C. albicans counts continuously increased in all of the cleanser-exposed biofilms, with abundant hyphae forms on SEM and CLSM images. The polysaccharide concentration was significantly higher in the experimental group after 7 days.
  
  
  CONCLUSIONS
  Daily exposure of a multispecies biofilm to a denture cleanser reduces the number of total microorganisms but favors C. albicans development.
  
  
  CONCLUSIONS
  Daily use of denture cleanser is an effective method for controlling bacteria in biofilm, but it can potentially select C. albicans, an important etiological agent of oral candidosis.
authors.affiliation: Department of Prosthodontics and Periodontology, Piracicaba Dental School, State University of Campinas, Avenida Limeira, 901, 13414-903, Piracicaba, SP, Brazil.
authors.forename: Sílvia Carneiro de
authors.initials: SC
authors.lastname: Lucena-Ferreira
authors.forename: Antonio Pedro
authors.initials: AP
authors.lastname: Ricomini-Filho
authors.forename: Wander José da
authors.initials: WJ
authors.lastname: Silva
authors.forename: Jaime Aparecido
authors.initials: JA
authors.lastname: Cury
authors.forename: Altair Antoninha Del Bel
authors.initials: AA
authors.lastname: Cury
mesh_terms: D018441:Biofilms / Q000187:drug effects*; D015169:Colony Count, Microbial; D003820:Denture Cleansers / Q000494:pharmacology*; D066298:In Vitro Techniques; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D013997:Time Factors
chemical_list: D003820:Denture Cleansers