title: Determination of platycodin D and platycodin D3 in rat plasma using liquid chromatography-tandem mass spectrometry.
abstract: Platycodon grandiflorum has long been used as a traditional oriental medicine for respiratory disorder. Platycodin D (PD) is known as the main component isolated from the root of PG. A simple and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the quantitation of PD in rat plasma. Quantitation was performed on a triple quadrupole mass spectrometer employing electrospray ionization and multiple reaction monitoring in positive ion mode. The total chromatographic run time was 4.0 min, and the calibration curves of PD were linear over the concentration range of 50-10,000 ng/mL in rat plasma. The coefficient of variation and relative error at five QC levels were 1.0 to 8.8% and 0.7 to 8.7%, respectively. After a single oral administration of 500 mg/kg and a single intravenous administration of 25 mg/kg of 3% PD extract (a PG extract including 3% of PD), platycodin D and platycodin D3 were detected and pharmacokinetic parameters were estimated. The oral bioavailability of platycodin D and platycodin D3 was 0.29% and 1.35% in rats at 500 mg/kg of 3% PD extract of PG, respectively. The present method can be applied to pharmacokinetic analysis of platycodins and platycosides of the PG.
authors.affiliation: Bioresourres Regional Innovation Center, Soonchunhyang University, Asan 336-745, Republic of Korea.
authors.forename: Tae-Hyun
authors.identifier: 0000-0001-9026-3901
authors.initials: TH
authors.lastname: Kim
authors.affiliation: Industry-Academy Cooperation Foundation, Soonchunhyang University, Asan 336-745, Republic of Korea.
authors.forename: Byung Eui
authors.initials: BE
authors.lastname: Lee
authors.affiliation: Korea Institute of Toxicology, Korea Research Institute of Chemical Technology, Daejeon 305-343, Republic of Korea.
authors.forename: Eun Joo
authors.initials: EJ
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Dankook University, Cheonan 330-714, Republic of Korea.
authors.forename: Yong Seok
authors.initials: YS
authors.lastname: Choi
authors.affiliation: Department of Pharmacology, College of Medicine, Dankook University, Cheonan 330-714, Republic of Korea.
authors.forename: Keun-Sung
authors.initials: KS
authors.lastname: Lee
authors.affiliation: Department of Pharmacology, College of Medicine, Dankook University, Cheonan 330-714, Republic of Korea ; Dankook Translational Research Center, Dankook University, Cheonan 330-714, Republic of Korea.
authors.forename: Hak Rim
authors.identifier: 0000-0003-3102-2272
authors.initials: HR
authors.lastname: Kim
authors.affiliation: Department of Pharmacology, College of Medicine, Dankook University, Cheonan 330-714, Republic of Korea ; Dankook Translational Research Center, Dankook University, Cheonan 330-714, Republic of Korea.
authors.forename: Hyung-Gun
authors.identifier: 0000-0002-7990-3442
authors.initials: HG
authors.lastname: Kim
mesh_terms: D000818:Animals; D001682:Biological Availability; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000097:blood* / Q000493:pharmacokinetics; D014315:Triterpenes / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D012503:Saponins; D014315:Triterpenes; C431483:platycodin D3