title: Arachidonic acid promotes migration and invasion through a PI3K/Akt-dependent pathway in MDA-MB-231 breast cancer cells.
abstract: Arachidonic acid (AA) is a common dietary n-6 cis polyunsaturated fatty acid that under physiological conditions is present in an esterified form in cell membrane phospholipids, however it might be present in the extracellular microenvironment. AA and its metabolites mediate FAK activation, adhesion and migration in MDA-MB-231 breast cancer cells. However, it remains to be investigated whether AA promotes invasion and the signal transduction pathways involved in migration and invasion. Here, we demonstrate that AA induces Akt2 activation and invasion in MDA-MB-231 cells. Akt2 activation requires the activity of Src, EGFR, and PIK3, whereas migration and invasion require Akt, PI3K, EGFR and metalloproteinases activity. Moreover, AA also induces NFκB-DNA binding activity through a PI3K and Akt-dependent pathway. Our findings demonstrate, for the first time, that Akt/PI3K and EGFR pathways mediate migration and invasion induced by AA in MDA-MB-231 breast cancer cells.
authors.affiliation: Departamento de Biologia Celular, Cinvestav-IPN, Av. IPN # 2508, San Pedro Zacatenco, Mexico DF 07360, Mexico.
authors.forename: Socrates
authors.initials: S
authors.lastname: Villegas-Comonfort
authors.affiliation: Departamento de Biologia Celular, Cinvestav-IPN, Av. IPN # 2508, San Pedro Zacatenco, Mexico DF 07360, Mexico.
authors.forename: Rocio
authors.initials: R
authors.lastname: Castillo-Sanchez
authors.affiliation: Departamento de Biologia Celular, Cinvestav-IPN, Av. IPN # 2508, San Pedro Zacatenco, Mexico DF 07360, Mexico.
authors.forename: Nathalia
authors.initials: N
authors.lastname: Serna-Marquez
authors.affiliation: Departamento de Biologia Celular, Cinvestav-IPN, Av. IPN # 2508, San Pedro Zacatenco, Mexico DF 07360, Mexico.
authors.forename: Pedro
authors.initials: P
authors.lastname: Cortes-Reynosa
authors.affiliation: Departamento de Biologia Celular, Cinvestav-IPN, Av. IPN # 2508, San Pedro Zacatenco, Mexico DF 07360, Mexico. Electronic address: jperez@cell.cinvestav.mx.
authors.forename: Eduardo Perez
authors.initials: EP
authors.lastname: Salazar
mesh_terms: D016718:Arachidonic Acid / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000201:enzymology* / Q000473:pathology; D002465:Cell Movement / Q000187:drug effects*; D005260:Female; D006801:Humans; D009361:Neoplasm Invasiveness; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D016718:Arachidonic Acid; D019869:Phosphatidylinositol 3-Kinases; C494921:AKT2 protein, human; D051057:Proto-Oncogene Proteins c-akt
keywords: Akt; Breast cancer; Invasion; Migration; PI3K