title: The stimulatory effect of different CpG oligonucleotides on the maturation of chicken bone marrow-derived dendritic cells.
abstract: CpG oligonucleotide (CpG-ODN) can exert an immunostimulatory effect on different types of immune cells such as dendritic cells (DC). The immunostimulatory activity of CpG-ODN is closely related to its nucleotide sequence and structural characteristics. In this study, we aimed at evaluating the stimulatory effects of different CpG-ODN on the maturation of chicken bone marrow-derived DC (BM-DC) in vitro. First, 4 CpG-ODN were designed. Then chicken bone marrow cells were extracted from tibia and femur and cultured in the RPMI 1640 medium with recombinant chicken granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4. After culture for 6 d, the cells were stimulated by different CpG-ODN or lipopolysaccharide for 24 h. Finally, the effects of different CpG-ODN on the maturation of chicken BM-DC were investigated by morphologic, phenotypic, and functional assays. The results showed that the cultured cells could display the typical DC morphology, and the CpG-ODN could efficiently stimulate the BM-DC to show the mature morphologic characteristics and upregulate the expression of cluster of differentiation (CD) 40 and CD86 molecules. In addition, after stimulation by CpG-ODN, the BM-DC could significantly induce T-cell proliferative response (P < 0.01). Among all the sequences, the stimulatory effect of CpG-ODN F3 with an addition of poly-guanosine strings at the 3' end was the best on the chicken BM-DC. In conclusion, this is the first report to demonstrate that different CpG-ODN have distinct stimulatory effects on the maturation of chicken BM-DC and CpG-ODN F3 with the best stimulatory effect can be a potent stimulant for the maturation of chicken BM-DC.
authors.affiliation: Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, 210095, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Fu
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Liang
authors.forename: Haihong
authors.initials: H
authors.lastname: Kang
authors.forename: Jian
authors.initials: J
authors.lastname: Lin
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Yu
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects*; D049109:Cell Proliferation; D002478:Cells, Cultured; D002645:Chickens; D003713:Dendritic Cells / Q000166:cytology / Q000502:physiology*; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D013047:Specific Pathogen-Free Organisms; D013601:T-Lymphocytes / Q000166:cytology / Q000502:physiology
chemical_list: D009838:Oligodeoxyribonucleotides
keywords: CpG oligonucleotide; bone marrow-derived dendritic cell; chicken