title: Fast, multi-dimensional and simultaneous kymograph-like particle dynamics (SkyPad) analysis.
abstract: BACKGROUND
  Kymograph analysis is a method widely used by researchers to analyze particle dynamics in one dimensional (1D) trajectories.
  
  
  RESULTS
  Here we provide a Visual Basic-coded algorithm to use as a Microsoft Excel add-in that automatically analyzes particles in 2D trajectories with all the advantages of kymograph analysis.
  
  
  CONCLUSIONS
  This add-in, which we named SkyPad, leads to significant time saving and higher accuracy of particle analysis. Finally, SkyPad can also be used for 3D trajectories analysis.
authors.affiliation: UMRS 787 INSERM, Université Pierre et Marie Curie Paris 6, Paris, France ; Groupe Hospitalier Pitié-Salpêtrière, Institut de Myologie, Paris, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Cadot
authors.affiliation: UMRS 787 INSERM, Université Pierre et Marie Curie Paris 6, Paris, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Gache
authors.affiliation: UMRS 787 INSERM, Université Pierre et Marie Curie Paris 6, Paris, France ; Groupe Hospitalier Pitié-Salpêtrière, Institut de Myologie, Paris, France.
authors.forename: Edgar R
authors.initials: ER
authors.lastname: Gomes
mesh_terms: D000465:Algorithms; D000818:Animals; D002478:Cells, Cultured; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007700:Kinetics; D007734:Kymography / Q000379:methods*; D051379:Mice; D012984:Software; D014584:User-Computer Interface