title: Genotypic analysis of meningococcal factor h-binding protein from non-culture clinical specimens.
abstract: Factor H-Binding Protein (fHbp) is an outer membrane protein antigen included in two novel meningococcal group B vaccines and, as such, is an important typing target. Approximately 50% of meningococcal disease cases in England and Wales are confirmed using real-time PCR on non-culture clinical specimens only. Protocols for typing fHbp from this subset of cases have not yet been established. Here we present a nested PCR-based assay designed to amplify and sequence fHbp from non-culture clinical specimens. From analytical sensitivity experiments carried out using diluted DNA extracts, an estimated analytical sensitivity limit of 6 fg/µL of DNA (<3 genome copies/µL) was calculated. The sensitivity of the assay was shown to be comparable to the ctrA-directed real-time PCR assay currently used to confirm invasive disease diagnoses from submitted clinical specimens. A panel of 96 diverse, patient-matched clinical specimen/isolate pairs from invasive disease cases was used to illustrate the breadth of strain coverage for the assay. All fHbp alleles sequenced from the isolates matched those derived from previous whole genome analyses. The first-round PCR primer binding sites are highly conserved, however an exceptional second-round PCR primer site mismatch in one validation isolate prevented amplification. In this case, amplification from the corresponding clinical specimen was achieved, suggesting that the use of a nested PCR procedure may compensate for any minor mismatches in round-two primer sites. The assay was successful at typing 91/96 (94.8%) of the non-culture clinical specimens in this study and exhibits sufficient sensitivity to type fHbp from the vast majority of non-culture clinical specimens received by the Meningococcal Reference Unit, Public Health England.
authors.affiliation: Manchester Medical Microbiology Partnership, Public Health England, Clinical Sciences Buildings, Manchester Royal Infirmary, Manchester, United Kingdom.
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Clark
authors.affiliation: Manchester Medical Microbiology Partnership, Public Health England, Clinical Sciences Buildings, Manchester Royal Infirmary, Manchester, United Kingdom.
authors.forename: Jay
authors.initials: J
authors.lastname: Lucidarme
authors.affiliation: Manchester Medical Microbiology Partnership, Public Health England, Clinical Sciences Buildings, Manchester Royal Infirmary, Manchester, United Kingdom.
authors.forename: Lynne S
authors.initials: LS
authors.lastname: Newbold
authors.affiliation: Manchester Medical Microbiology Partnership, Public Health England, Clinical Sciences Buildings, Manchester Royal Infirmary, Manchester, United Kingdom.
authors.forename: Ray
authors.initials: R
authors.lastname: Borrow
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D017242:Complement Factor H / Q000378:metabolism*; D004739:England; D006801:Humans; D008589:Meningococcal Infections / Q000175:diagnosis*; D022401:Meningococcal Vaccines / Q000737:chemistry*; D008969:Molecular Sequence Data; D009345:Neisseria meningitidis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D014852:Wales
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D022401:Meningococcal Vaccines; C484699:factor H-binding protein, Neisseria meningitidis; D017242:Complement Factor H