title: Identification of dermatophyte species using genomic in situ hybridization (GISH).
abstract: A genomic in situ hybridization (GISH)-based method for dermatophyte identification has been developed. Using specific GISH probes, discrimination between Trichophyton interdigitale, Trichophyton rubrum and Microsporum canis has been conducted. Moreover, GISH has been found particularly helpful when proper dermatophyte identification was difficult due to ambiguous PCR-RFLP patterns.
authors.affiliation: Department of Genetics, University of Rzeszow, Kolbuszowa, Poland.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Worek
authors.affiliation: Department of Botany and Biotechnology of Economic Plants, University of Rzeszow, Kolbuszowa, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Kwiatkowska
authors.affiliation: Department of Microbial Genetics, University of Lodz, Lodz, Poland.
authors.forename: Anita
authors.initials: A
authors.lastname: Ciesielska
authors.affiliation: University of Social Sciences, Lodz, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Jaworski
authors.affiliation: Department of Genetics, University of Rzeszow, Kolbuszowa, Poland.
authors.forename: Jakub
authors.initials: J
authors.lastname: Kaplan
authors.affiliation: Department of Genetics, University of Rzeszow, Kolbuszowa, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Miedziak
authors.affiliation: Department of Genetics, University of Rzeszow, Kolbuszowa, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Deregowska
authors.affiliation: Department of Biochemistry and Cell Biology, University of Rzeszow, Rzeszow, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Lewinska
authors.affiliation: Department of Genetics, University of Rzeszow, Kolbuszowa, Poland. Electronic address: mawnuk@gmail.com.
authors.forename: Maciej
authors.initials: M
authors.lastname: Wnuk
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D003883:Arthrodermataceae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D003881:Dermatomycoses / Q000175:diagnosis* / Q000382:microbiology; D005260:Female; D016681:Genome, Fungal; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D008297:Male; D008864:Microsporum / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D008875:Middle Aged; D014249:Trichophyton / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D055815:Young Adult
keywords: Dermatophytes; GISH; Microsporum; PCR–RFLP; Trichophyton