title: Do PFT1 and HY5 interact in regulation of sulfate assimilation by light in Arabidopsis?
abstract: Sulfate assimilation pathway is highly responsive to changes in environment, but the mechanisms of such regulation are only slowly beginning to unravel. Here we show evidence that PHYTOCHROME AND FLOWERING TIME 1 (PFT1) may be another component of the sulfate assimilation regulatory circuit. Transcriptional regulation by light of the key enzyme of sulfate assimilation, adenosine 5'phosphosulfate (APS) reductase, is disturbed in pft1-2 mutants. PFT1, however, affects also APS reductase enzyme activity, flux through the sulfate assimilation pathway and accumulation of glutathione. In addition, our data suggest a possible interplay of PFT1 with another transcription factor, HY5, in regulation of APS reductase by light.
authors.affiliation: Department of Metabolic Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
authors.forename: Anna
authors.initials: A
authors.lastname: Koprivova
authors.affiliation: Department of Metabolic Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
authors.forename: Alexander
authors.initials: A
authors.lastname: Calderwood
authors.affiliation: Department of Metabolic Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
authors.forename: Bok-Rye
authors.initials: BR
authors.lastname: Lee
authors.affiliation: Department of Metabolic Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK. Electronic address: skopriva@uni-koeln.de.
authors.forename: Stanislav
authors.initials: S
authors.lastname: Kopriva
mesh_terms: D017360:Arabidopsis / Q000378:metabolism* / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins; D004790:Enzyme Induction / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D005978:Glutathione / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000235:genetics / Q000378:metabolism; D013430:Sulfate Adenylyltransferase / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; D004268:DNA-Binding Proteins; C109254:HY5 protein, Arabidopsis; D009687:Nuclear Proteins; C475686:PFT1 protein, Arabidopsis; D013431:Sulfates; D050862:Oxidoreductases Acting on Sulfur Group Donors; C019618:adenylylsulfate reductase; D013430:Sulfate Adenylyltransferase; D005978:Glutathione
keywords: Adenosine 5′phosphosulfate reductase; Arabidopsis thaliana; LONG HYPOCOTYL 5; Light signaling; PHYTOCHROME AND FLOWERING TIME 1; Sulfate assimilation