title: Alternative-splicing-mediated gene expression.
abstract: Alternative splicing (AS) is a fundamental process during gene expression and has been found to be ubiquitous in eukaryotes. However, how AS impacts gene expression levels both quantitatively and qualitatively remains to be fully explored. Here, we analyze two common models of gene expression, each incorporating a simple splice mechanism that a pre-mRNA is spliced into two mature mRNA isoforms in a probabilistic manner. In the constitutive expression case, we show that the steady-state molecular numbers of two mature mRNA isoforms follow mutually independent Poisson distributions. In the bursting expression case, we demonstrate that the tail decay of the steady-state distribution for both mature mRNA isoforms that in general are not mutually independent can be characterized by the product of mean burst size and splicing probability. In both cases, we find that AS can efficiently modulate both the variability (measured by variance) and the noise level of the total mature mRNA, and in particular, the latter is always lower than the noise level of the pre-mRNA, implying that AS always reduces the noise. These results altogether reveal that AS is a mechanism of efficiently controlling the gene expression noise.
authors.affiliation: School of Mathematics and Computational Science, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Qianliang
authors.initials: Q
authors.lastname: Wang
authors.affiliation: School of Mathematics and Computational Science, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China and Guangdong Province Key Laboratory of Computational Science and School of Mathematics and Computational Science, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Tianshou
authors.initials: T
authors.lastname: Zhou
mesh_terms: D017398:Alternative Splicing / Q000235:genetics*; D001665:Binding Sites; D003198:Computer Simulation; D005786:Gene Expression Regulation / Q000235:genetics*; D008956:Models, Chemical; D008957:Models, Genetic; D022821:RNA Splice Sites / Q000235:genetics*; D012333:RNA, Messenger / Q000737:chemistry* / Q000235:genetics*
chemical_list: D022821:RNA Splice Sites; D012333:RNA, Messenger