title: Liver X receptor activation induces apoptosis of melanoma cell through caspase pathway.
abstract: Liver X receptors (LXRs) are nuclear receptors that function as ligand-activated transcription factors regulating lipid metabolism and inflammation. Recent discoveries found LXRs could regulate tumor growth in a variety of cancer cell lines. In this study, we investigated the effect of LXR activation on melanoma cell proliferation and apoptosis both in vitro and in vivo. Treatment of B16F10 and A-375 melanoma cells with synthetic LXR agonist T0901317 significantly inhibited the proliferation of melanoma cells in vitro. Meanwhile, T0901317 induced the apoptosis of B16F10 melanoma cells in a dose-dependent manner. Furthermore, western blot assay showed that the pro-apoptotic effect of T0901317 on B16F10 melanoma cells was mediated through caspase-3 pathway. Oral administration of T0901317 inhibited the growth of B16F10 melanoma in C56BL/6 mice. Altogether, this study demonstrates the critical role of LXRs in the regulation of melanoma growth and presents the LXR agonist T0901317 as a potential anti-melanoma agent.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Plastic Surgery, Changzheng Hospital, 18F, No, 415 Fengyang Road, Shanghai, China. drjianghua@yeah.net.
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
authors.forename: Jianlin
authors.initials: J
authors.lastname: Zhang
authors.forename: Yinfan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Antang
authors.initials: A
authors.lastname: Liu
authors.forename: Yaozhong
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xiaohai
authors.initials: X
authors.lastname: Zhu
authors.forename: Zihao
authors.initials: Z
authors.lastname: Lin
authors.forename: Xiangbin
authors.initials: X
authors.lastname: Yuan