title: A multi-species bait for Chagas disease vectors.
abstract: BACKGROUND
  Triatomine bugs are the insect vectors of Trypanosoma cruzi, the etiological agent of Chagas disease. These insects are known to aggregate inside shelters during daylight hours and it has been demonstrated that within shelters, the aggregation is induced by volatiles emitted from bug feces. These signals promote inter-species aggregation among most species studied, but the chemical composition is unknown.
  
  
  RESULTS
  In the present work, feces from larvae of the three species were obtained and volatile compounds were identified by solid phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS). We identified five compounds, all present in feces of all of the three species: Triatoma infestans, Panstrongylus megistus and Triatoma brasiliensis. These substances were tested for attractivity and ability to recruit insects into shelters. Behaviorally active doses of the five substances were obtained for all three triatomine species. The bugs were significantly attracted to shelters baited with blends of 160 ng or 1.6 µg of each substance.
  
  
  CONCLUSIONS
  Common compounds were found in the feces of vectors of Chagas disease that actively recruited insects into shelters, which suggests that this blend of compounds could be used for the development of baits for early detection of reinfestation with triatomine bugs.
authors.affiliation: Laboratório de Triatomíneos e Epidemiologia da Doença de Chagas, CPqRR-FIOCRUZ, Belo Horizonte, Brazil ; Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas-UFMG, Belo Horizonte, Brazil.
authors.forename: Theo
authors.initials: T
authors.lastname: Mota
authors.affiliation: Laboratório de Triatomíneos e Epidemiologia da Doença de Chagas, CPqRR-FIOCRUZ, Belo Horizonte, Brazil.
authors.forename: Ana C R
authors.initials: AC
authors.lastname: Vitta
authors.affiliation: Laboratorio de Fisiología de Insectos, IBBEA-CONICET, FCEyN, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Alicia N
authors.initials: AN
authors.lastname: Lorenzo-Figueiras
authors.affiliation: Laboratório de Triatomíneos e Epidemiologia da Doença de Chagas, CPqRR-FIOCRUZ, Belo Horizonte, Brazil.
authors.forename: Carla P
authors.initials: CP
authors.lastname: Barezani
authors.affiliation: Laboratório de Química de Produtos Naturais, CPqRR-FIOCRUZ, Belo Horizonte, Brazil.
authors.forename: Carlos L
authors.initials: CL
authors.lastname: Zani
authors.affiliation: Institut de Recherche sur la Biologie de l'Insecte, UMR CNRS 7261, Université François Rabelais, Tours, France.
authors.forename: Claudio R
authors.initials: CR
authors.lastname: Lazzari
authors.affiliation: Laboratório de Triatomíneos e Epidemiologia da Doença de Chagas, CPqRR-FIOCRUZ, Belo Horizonte, Brazil.
authors.forename: Liléia
authors.initials: L
authors.lastname: Diotaiuti
authors.affiliation: Laboratório de Triatomíneos e Epidemiologia da Doença de Chagas, CPqRR-FIOCRUZ, Belo Horizonte, Brazil.
authors.forename: Lynne
authors.initials: L
authors.lastname: Jeffares
authors.affiliation: Laboratório de Triatomíneos e Epidemiologia da Doença de Chagas, CPqRR-FIOCRUZ, Belo Horizonte, Brazil.
authors.forename: Björn
authors.initials: B
authors.lastname: Bohman
authors.affiliation: Laboratório de Triatomíneos e Epidemiologia da Doença de Chagas, CPqRR-FIOCRUZ, Belo Horizonte, Brazil.
authors.forename: Marcelo G
authors.initials: MG
authors.lastname: Lorenzo
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D014355:Chagas Disease / Q000517:prevention & control*; D005243:Feces / Q000737:chemistry*; D007300:Insect Control / Q000379:methods*; D007303:Insect Vectors / Q000187:drug effects / Q000502:physiology; D007814:Larva; D010203:Panstrongylus / Q000187:drug effects / Q000502:physiology; D010675:Pheromones; D014224:Triatoma / Q000187:drug effects / Q000502:physiology; D055549:Volatile Organic Compounds / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D010675:Pheromones; D055549:Volatile Organic Compounds