title: Label-free fluorescent biosensor based on the target recycling and Thioflavin T-induced quadruplex formation for short DNA species of c-erbB-2 detection.
abstract: Non-invasive early diagnosis of breast cancer is the most effective way to improve the survival rate and increase more chances of breast-conserving. In this paper, we developed a label-free fluorescent biosensor based on nuclease assisted target recycling and Thioflavin T-induced quadruplex formation for short DNA species of c-erbB-2 detection in saliva. By employing the strategy, the sensor can detect as low as 20fM target DNA with high discrimination ability even against single-base mismatch sequence. To the best of our knowledge, the proposed sensor is the first attempt to apply Thioflavin T that possesses outstanding structural selectivity for G-quadruplex in DNA amplification techniques, which may represent a promising path toward direct breast cancer detection in saliva at the point of care.
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350004, China.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350004, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Lin
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350004, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350004, China.
authors.forename: Shuxian
authors.initials: S
authors.lastname: Cai
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350004, China.
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350004, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Medical Oncology, Fujian Medical University Union Hospital, Fujian Key Laboratory of Tumor Translational Medicine, Fuzhou 350001, China. Electronic address: yangshjxh@126.com.
authors.forename: Sheng
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Chemistry and Biology, Faculty of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China. Electronic address: 1601225385@qq.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
mesh_terms: D020137:Base Pair Mismatch; D052160:Benzothiazoles; D015374:Biosensing Techniques / Q000379:methods*; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D004247:DNA / Q000032:analysis*; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D054856:G-Quadruplexes; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics* / Q000302:isolation & purification; D012463:Saliva / Q000378:metabolism; D013844:Thiazoles / Q000737:chemistry*
chemical_list: D052160:Benzothiazoles; D004247:DNA; D005456:Fluorescent Dyes; D018719:Erb-b2 Receptor Tyrosine Kinases; D013844:Thiazoles; C009462:thioflavin T
keywords: Fluorescent biosensor; Quadruplex; Saliva; Target recycling; Thioflavin T; c-erbB-2