title: Halocynthiibacter namhaensis gen. nov., sp. nov., a novel alphaproteobacterium isolated from sea squirt Halocynthia roretzi.
abstract: A Gram-negative, non-motile and rod-shaped bacterial strain, designated RA2-3(T), was isolated from a sea squirt (Halocynthia roretzi) collected from the South Sea, Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain RA2-3(T) was observed to grow optimally at 25 °C, at pH 7.0-7.5 and in the presence of 2 % (w/v) NaCl. Strain RA2-3(T) exhibited the highest 16S rRNA gene sequence similarity values to the type strains of Litoreibacter meonggei (95.7 %), Planktotalea frisia (95.6 %), Thalassobius gelatinovorus (95.5 %) and Pelagicola litoralis (95.4 %). A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain RA2-3(T) clustered with the type strains of Planktotalea frisia, Pelagicola litoralis, Pacificibacter maritimus and Roseovarius marinus. Strain RA2-3(T) was found to contain Q-10 as the predominant ubiquinone and C18:1 ω7c as the major fatty acid. The major polar lipids detected in strain RA2-3(T) were phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain RA2-3(T) was 52.9 mol%. On the basis of the phylogenetic, chemotaxonomic and phenotypic properties, strain RA2-3(T) is considered to represent a new genus and species within the family Rhodobacteraceae, for which the name Halocynthiibacter namhaensis gen. nov., sp. nov. is proposed. The type strain of H. namhaensis is RA2-3(T) (=KCTC 32362(T)=NBRC 109999(T)).
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute (NFRDI), Gijang, Busan, 619-705, South Korea.
authors.forename: Young-Ok
authors.initials: YO
authors.lastname: Kim
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Park
authors.forename: Hyangmi
authors.initials: H
authors.lastname: Kim
authors.forename: Doo-Sang
authors.initials: DS
authors.lastname: Park
authors.forename: Bo-Hye
authors.initials: BH
authors.lastname: Nam
authors.forename: Dong-Gyun
authors.initials: DG
authors.lastname: Kim
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D000818:Animals; D059001:Aquatic Organisms / Q000382:microbiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D007723:Korea; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D017422:Sequence Analysis, DNA; D014561:Urochordata / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S