title: [Influence of ablactation rats' high calcium intake to the expression of uncoupling protein 2 mRNA after adulthood obesity induction].
abstract: OBJECTIVE
  To study the influence of early high calcium intake to adulthood obesity through detection the expression of uncoupling protein 2 ( UCP2) mRNA in muscle by a reverse transcription polymerase chain reaction ( RT-PCR) technique.
  
  
  METHODS
  120 male Wistar rats were divided randomly into normal control group, high dose calcium group, medium dose calcium group and low doses calcium group, with basic diet and high calcium diet for 4 weeks. After intervention, blood fat was compared. Subsequently, all rats were fed basic diet for 3 weeks and blood fat was compared. Then, normal feed rats were randomly divided into normal controls and obesity induction group. Obesity induction group, high dose calcium group, medium dose calcium group and low doses calcium group were fed high-fat food. After 8 weeks, RT-PCR was used for analysis After obesity induction, weight the expression of UCP2 mRNA in muscle.
  
  
  RESULTS
  growth of three high calcium groups were below obesity induction group, weight growth of low doses and high doses of high calcium groups were no difference with normal control group. Serum triglyceride levels of high dose high calcium group were significantly lower than obesity induction group and no difference with normal control group. Expression level of UCP2 mRNA of obesity induction group and high doses of high calcium group were obviously lower than normal controls, low dose and medium dose high calcium group, medium dose high calcium group' expression was significantly higher than normal control group.
  
  
  CONCLUSIONS
  In rats' early life high calcium intake can continue to affect adulthood obesity induced by high-fat feed, increase expression level of UCP2 mRNA, improve the disorder of blood fat metabolism.
authors.affiliation: College of Public Health, Xinjiang Medical University, Urumqi 830054, China.
authors.forename: Shudi
authors.initials: S
authors.lastname: Xu
authors.affiliation: College of Public Health, Xinjiang Medical University, Urumqi 830054, China.
authors.forename: Keyuan
authors.initials: K
authors.lastname: Zhang
authors.affiliation: College of Public Health, Xinjiang Medical University, Urumqi 830054, China.
authors.forename: Shutao
authors.initials: S
authors.lastname: Zheng
authors.affiliation: College of Public Health, Xinjiang Medical University, Urumqi 830054, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Public Health, Xinjiang Medical University, Urumqi 830054, China.
authors.forename: Libo
authors.initials: L
authors.lastname: Yu
authors.affiliation: College of Public Health, Xinjiang Medical University, Urumqi 830054, China.
authors.forename: Hiong
authors.initials: H
authors.lastname: Ding
mesh_terms: D000818:Animals; D002136:Calcium, Dietary / Q000008:administration & dosage*; D004041:Dietary Fats / Q000008:administration & dosage*; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism*; D008055:Lipids / Q000097:blood; D008297:Male; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism; D009765:Obesity / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D000071246:Uncoupling Protein 2; D014886:Weaning
chemical_list: D002136:Calcium, Dietary; D004041:Dietary Fats; D007473:Ion Channels; D008055:Lipids; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; C000604391:Ucp2 protein, rat; D000071246:Uncoupling Protein 2