title: Upregulation of microRNA-25 associates with prognosis in hepatocellular carcinoma.
abstract: BACKGROUND
  Accumulating evidence has shown that up-regulation of microRNA-25(miR-25) is associated with the prognosis of several types of human malignant solid tumors. However, whether miR-25 expression has influence on the prognosis of hepatocellular carcinoma (HCC) is still unknown.
  
  
  METHODS
  The differentially expressed amount of the miR-25 was validated in triplicate by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Survival rate was analyzed by log-rank test, and survival curves were plotted according to Kaplan-Meier. Multivariate analysis of the prognostic factors was performed with Cox regression model.
  
  
  RESULTS
  The expression of miR-25 was significantly upregulated in HCC tissues when compared with adjacent normal tissues (p<0.0001). Patients who had high miR-25 expression had a shorter overall survival than patients who had low miR-25 expression (median overall survival, 31.0 months versus 42.9 months, p=0.0192). The multivariate Cox regression analysis indicated that miR-25 expression (HR=2.179; p=0.001), TNM stage (HR=1.782; p=0.014), and vein invasion (HR=1.624; p=0.020) were independent prognostic factors for overall survival.
  
  
  CONCLUSIONS
  Our data suggests that the overexpression of miR-25 in HCC tissues is of predictive value on poor prognosis.
  The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1989618421114309.
authors.affiliation: Department of Hepatobiliary Surgery, ShanDong Provincial Hospital affiliated to ShanDong University, 324 JingWu Road, Jinan, ShanDong Province 250021, China. dr_suzhongxue@126.com.
authors.forename: Zhong-xue
authors.initials: ZX
authors.lastname: Su
authors.forename: Juan
authors.initials: J
authors.lastname: Zhao
authors.forename: Zhong-hou
authors.initials: ZH
authors.lastname: Rong
authors.forename: Wen-mao
authors.initials: WM
authors.lastname: Geng
authors.forename: Ya-guang
authors.initials: YG
authors.lastname: Wu
authors.forename: Cheng-kun
authors.initials: CK
authors.lastname: Qin
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000401:mortality*; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000401:mortality*; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D011379:Prognosis; D016016:Proportional Hazards Models; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C543351:MIRN25 microRNA, human; D035683:MicroRNAs