title: A new method for the preservation of axenic fungal cultures.
abstract: Microbiological work requires a reliable source of cultures that are not only well defined and taxonomically determined, but are also adequately preserved without changes in their morphological, physiological and genetic traits. Here we describe an easy, cost effective and rapid method for reliably preserving filamentous fungi on cellophane pieces at -80°C for use in laboratory culture collections.
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China.
authors.forename: Xiaojia
authors.initials: X
authors.lastname: Hu
authors.affiliation: Cardiff School of Biosciences, Cardiff University, Main Building, Park Place, Cardiff, Wales CF10 3AT, UK.
authors.forename: Gordon
authors.initials: G
authors.lastname: Webster
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Xie
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China.
authors.forename: Changbing
authors.initials: C
authors.lastname: Yu
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China.
authors.forename: Yinshui
authors.initials: Y
authors.lastname: Li
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China. Electronic address: xinliao1963@sina.cn.
authors.forename: Xing
authors.initials: X
authors.lastname: Liao
mesh_terms: D005615:Freezing; D005658:Fungi / Q000302:isolation & purification* / Q000502:physiology*; D009172:Mycology / Q000379:methods*; D011309:Preservation, Biological / Q000379:methods*
keywords: Aspergillus; Cryopreservation; Culture collection; Fungi; Trichoderma