title: Self-immolative bioluminogenic quinone luciferins for NAD(P)H assays and reducing capacity-based cell viability assays.
abstract: Highly sensitive self-cleavable trimethyl lock quinone-luciferin substrates for diaphorase were designed and synthesized to measure NAD(P)H in biological samples and monitor viable cells via NAD(P)H-dependent cellular oxidoreductase enzymes and their NAD(P)H cofactors.
authors.affiliation: Research and Development, Promega Biosciences, Inc. 277 Granada Drive, San Luis Obispo, CA 93401 (USA). wenhui.zhou@promega.com.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Zhou
authors.forename: Donna
authors.initials: D
authors.lastname: Leippe
authors.forename: Sarah
authors.initials: S
authors.lastname: Duellman
authors.forename: Mary
authors.initials: M
authors.lastname: Sobol
authors.forename: Jolanta
authors.initials: J
authors.lastname: Vidugiriene
authors.forename: Martha
authors.initials: M
authors.lastname: O'Brien
authors.forename: John W
authors.initials: JW
authors.lastname: Shultz
authors.forename: Joshua J
authors.initials: JJ
authors.lastname: Kimball
authors.forename: Céline
authors.initials: C
authors.lastname: DiBernardo
authors.forename: Leonard
authors.initials: L
authors.lastname: Moothart
authors.forename: Laurent
authors.initials: L
authors.lastname: Bernad
authors.forename: James
authors.initials: J
authors.lastname: Cali
authors.forename: Dieter H
authors.initials: DH
authors.lastname: Klaubert
authors.forename: Poncho
authors.initials: P
authors.lastname: Meisenheimer
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival; D049454:Firefly Luciferin / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D006801:Humans; D049408:Luminescent Agents / Q000032:analysis / Q000378:metabolism*; D008163:Luminescent Measurements; D009249:NADP / Q000032:analysis / Q000378:metabolism*; D011809:Quinones / Q000032:analysis / Q000378:metabolism*
chemical_list: D049408:Luminescent Agents; D011809:Quinones; D009249:NADP; D049454:Firefly Luciferin
keywords: NAD(P)H; diaphorase; quinones; reducing capacity; self-immolative linkers