title: Identification of CMY-2-type cephalosporinases in clinical isolates of Enterobacteriaceae by MALDI-TOF MS.
abstract: This study exploited the possibility to detect Citrobacter freundii-derived CMY-2-like cephalosporinases in Enterobacteriaceae clinical isolates using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Periplasmic proteins were prepared using a modified sucrose method and analyzed by MALDI-TOF MS. A ca. 39,850-m/z peak, confirmed to represent a C. freundii-like β-lactamase by in-gel tryptic digestion followed by MALDI-TOF/TOF MS, was observed only in CMY-producing isolates. We have also shown the potential of the assay to detect ACC- and DHA-like AmpC-type β-lactamases.
authors.affiliation: Department of Microbiology, Faculty of Medicine and University Hospital in Plzen, Charles University in Prague, Plzen, Czech Republic.
authors.forename: C C
authors.initials: CC
authors.lastname: Papagiannitsis
authors.forename: S D
authors.initials: SD
authors.lastname: Kotsakis
authors.forename: Z
authors.initials: Z
authors.lastname: Tuma
authors.forename: M
authors.initials: M
authors.lastname: Gniadkowski
authors.forename: V
authors.initials: V
authors.lastname: Miriagou
authors.forename: J
authors.initials: J
authors.lastname: Hrabak
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D002510:Cephalosporinase / Q000378:metabolism*; D004755:Enterobacteriaceae / Q000201:enzymology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D002510:Cephalosporinase