title: Quantitative field testing Heterodera glycines from metagenomic DNA samples isolated directly from soil under agronomic production.
abstract: A quantitative PCR procedure targeting the Heterodera glycines ortholog of the Caenorhabditis elegans uncoordinated-78 gene was developed. The procedure estimated the quantity of H. glycines from metagenomic DNA samples isolated directly from field soil under agronomic production. The estimation of H. glycines quantity was determined in soil samples having other soil dwelling plant parasitic nematodes including Hoplolaimus, predatory nematodes including Mononchus, free-living nematodes and biomass. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from field soil.
authors.affiliation: Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, Mississippi, United States of America.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, Mississippi, United States of America.
authors.forename: Gary W
authors.initials: GW
authors.lastname: Lawrence
authors.affiliation: Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, Mississippi, United States of America.
authors.forename: Shien
authors.initials: S
authors.lastname: Lu
authors.affiliation: Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, Mississippi, United States of America.
authors.forename: Clarissa
authors.initials: C
authors.lastname: Balbalian
authors.affiliation: Department of Biological Sciences, Mississippi State University, Mississippi State, Mississippi, United States of America.
authors.forename: Vincent P
authors.initials: VP
authors.lastname: Klink
mesh_terms: D000818:Animals; D001483:Base Sequence; D029742:Caenorhabditis elegans Proteins / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D054892:Metagenome / Q000235:genetics*; D008840:Microfilament Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry* / Q000469:parasitology*; D013045:Species Specificity; D014431:Tylenchoidea / Q000235:genetics*
chemical_list: D029742:Caenorhabditis elegans Proteins; D017931:DNA Primers; D008840:Microfilament Proteins; D012987:Soil; C423987:unc-78 protein, C elegans