title: Strength training alters MCT1-protein expression and exercise-induced translocation in erythrocytes of men with non-insulin-dependent type-2 diabetes.
abstract: We investigated the cellular distribution of lactate transporter (MCT1) and its chaperone CD147 (using immunohistochemistry and fluorescence-activated cell sorting) in the erythrocytes of men with non-insulin-dependent type-2 diabetes (NIDDM, n = 11, 61 ± 8 years of age) under acute exercise (ergometer cycling test, World Health Organisation scheme) performed before and after a 3-month strength training program. Cytosolic MCT1 distribution and membraneous CD147 density did not change after acute exercise (ergometer). After the 3-month strength training, MCT1-density was increased and the reaction of MCT1 (but not that of CD147) towards acute exercise (ergometer) was altered. MCT1 localisation was shifted from the centre to the cellular membrane. This resulted in a decrease in the immunohistochemically measured cytosolic MCT1-density. We conclude that strength training alters the acute exercise reaction of MCT1 but not that of CD147 in erythrocytes in patients with NIDDM. This reaction may contribute to long-term normalisation and stabilisation of the regulation of lactate plasma concentration in NIDDM.
authors.affiliation: a Department of Molecular and Cellular Sport Medicine, German Sport University, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany.
authors.forename: David
authors.initials: D
authors.lastname: Opitz
authors.forename: Thorsten
authors.initials: T
authors.lastname: Kreutz
authors.forename: Edward
authors.initials: E
authors.lastname: Lenzen
authors.forename: Benedict
authors.initials: B
authors.lastname: Dillkofer
authors.forename: Patrick
authors.initials: P
authors.lastname: Wahl
authors.forename: Gracia
authors.initials: G
authors.lastname: Montiel-Garcia
authors.forename: Christine
authors.initials: C
authors.lastname: Graf
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Bloch
authors.forename: Klara
authors.initials: K
authors.lastname: Brixius
mesh_terms: D000368:Aged; D051926:Basigin / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000097:blood; D008297:Male; D008875:Middle Aged; D027501:Monocarboxylic Acid Transporters / Q000378:metabolism*; D021381:Protein Transport; D055070:Resistance Training; D027981:Symporters / Q000378:metabolism*; D000099233:Monocarboxylate Transport Protein 1
chemical_list: D051926:Basigin; D007328:Insulin; D027501:Monocarboxylic Acid Transporters; D027981:Symporters; D000099233:Monocarboxylate Transport Protein 1; C497886:BSG protein, human