title: Differential regulation of lipid metabolism genes in the brain of acetylcholinesterase knockout mice.
abstract: Mice deficient in acetylcholinesterase (AChE; EC3.1.1.7) exhibited significant phenotypical and biochemical changes when compared with wild-type littermates. They showed a delay of growth in weight and size, immature external ears, and persistent body tremor, and they circled when walking. The molecular mechanisms underlying these changes have not been investigated yet. Here, we studied the profiles of both the messenger RNA (mRNA) and protein expression in the brain of AChE-deficient mice using mRNA microarray, quantitative PCR, and two-dimensional difference gel electrophoresis (2D DIGE) coupled to protein identification with matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry. Analysis of gene expression profile was conducted by DAVID ( http://david.abcc.ncifcrf.gov ) and Ingenuity Pathway Analysis (IPA, http://www.ingenuity.com ). Previous results implicated that there is a close relationship between lipid metabolisms which were associated with central nervous system development. Here, we demonstrated that the mRNA expressions of brain specific fatty acid protein 7 (fabp-7) and phospholipase A2 group IV (pla2g4) were significantly downregulated in AChE-deficient mice. These results suggested that AChE may play a role in neurogenesis and neurodegeneration by specifically regulating lipid metabolism in the brain.
authors.affiliation: School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, SAR, China, b113369@cuhk.edu.hk.
authors.forename: Huang-Quan
authors.initials: HQ
authors.lastname: Lin
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Kam-Leung
authors.initials: KL
authors.lastname: Chan
authors.forename: Tsz-Ming
authors.initials: TM
authors.lastname: Ip
authors.forename: Chi-Cheong David
authors.initials: CC
authors.lastname: Wan
mesh_terms: D000110:Acetylcholinesterase / Q000235:genetics*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D015536:Down-Regulation; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism; D058851:GPI-Linked Proteins / Q000235:genetics; D054513:Group IV Phospholipases A2 / Q000235:genetics / Q000378:metabolism; D050356:Lipid Metabolism; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: C086936:Fabp7 protein, mouse; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D058851:GPI-Linked Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D054513:Group IV Phospholipases A2; D000110:Acetylcholinesterase; C548245:Ache protein, mouse