title: Profiling of low molecular weight proteins in plasma from locally irradiated individuals.
abstract: In studies reported in the 1960s and since, blood plasma from radiation-exposed individuals has been shown to induce chromosome damage when transferred into lymphocyte cultures of non-irradiated persons. This effect has been described to occur via clastogenic factors, whose nature is still mostly unknown. We have previously examined clastogenic factors from irradiated individuals by looking at plasma-induced DNA damage in reporter cells. Plasma was tested from ca. 30 locally exposed clinical patients receiving fractionated radiation treatment, as well as from three radiological accident victims exposed in 1994, albeit sampled 14 years post-accident. In the current work, proteome changes in the plasma from all subjects were examined with 2D gel electrophoresis-based proteomics techniques, in order to evaluate the level of protein expression with respect to the findings of a clastogenic factor effect. No differences were observed in protein expression due to local radiation exposure (pre- vs post-exposure). In contrast, plasma from the radiation accident victims showed alterations in the expression of 18 protein spots (in comparison with plasma from the control group). Among these, proteins such as haptoglobin, serotransferrin/transferrin, fibrinogen and ubiquitin-60S ribosomal protein L40 were observed, none of them likely to be clastogenic factors. In conclusion, the proteomics techniques applied were unable to identify changes in the proteome of the locally irradiated patients, whereas such differences were observed for the accident victims. However, association with the clastogenic effect or any specific clastogenic factor remains unresolved and thus further studies with more sensitive techniques are warranted.
authors.affiliation: STUK - Radiation and Nuclear Safety Authority, Laippatie 4, PO Box 14, 00881 Helsinki, Finland reetta.nylund@stuk.fi.
authors.forename: Reetta
authors.initials: R
authors.lastname: Nylund
authors.affiliation: STUK - Radiation and Nuclear Safety Authority, Laippatie 4, PO Box 14, 00881 Helsinki, Finland.
authors.forename: Elina
authors.initials: E
authors.lastname: Lemola
authors.affiliation: Institute for Clinical Biochemistry and Pathobiochemistry, German Diabetes Center, Auf'm Hennekamp 65, 40225 Düsseldorf, Germany.
authors.forename: Sonja
authors.initials: S
authors.lastname: Hartwig
authors.affiliation: Institute for Clinical Biochemistry and Pathobiochemistry, German Diabetes Center, Auf'm Hennekamp 65, 40225 Düsseldorf, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Lehr
authors.affiliation: STUK - Radiation and Nuclear Safety Authority, Laippatie 4, PO Box 14, 00881 Helsinki, Finland.
authors.forename: Anna
authors.initials: A
authors.lastname: Acheva
authors.affiliation: Department of Radiotherapy and Radiation Oncology, University of Leipzig, Stephanstrasse 9a, 04103 Leipzig, Germany.
authors.forename: Jutta
authors.initials: J
authors.lastname: Jahns
authors.affiliation: Department of Radiotherapy and Radiation Oncology, University of Leipzig, Stephanstrasse 9a, 04103 Leipzig, Germany Department of Radiotherapy, University of Rostock, Suedring 75, 18059 Rostock, Germany.
authors.forename: Guido
authors.initials: G
authors.lastname: Hildebrandt
authors.affiliation: STUK - Radiation and Nuclear Safety Authority, Laippatie 4, PO Box 14, 00881 Helsinki, Finland.
authors.forename: Carita
authors.initials: C
authors.lastname: Lindholm
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood / Q000737:chemistry; D001798:Blood Proteins / Q000737:chemistry / Q000528:radiation effects*; D016022:Case-Control Studies; D004249:DNA Damage; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D009153:Mutagens / Q000737:chemistry / Q000528:radiation effects; D020543:Proteome / Q000737:chemistry / Q000528:radiation effects; D018788:Radioactive Hazard Release; D011878:Radiotherapy / Q000009:adverse effects*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D009153:Mutagens; D020543:Proteome
keywords: clastogenic factors; ionizing radiation; low molecular weight plasma proteins; radiation proteomics; two-dimensional difference gel electrophoresis (2DE-DIGE)