title: Fluorescence detection of polyadenylation reaction through the coordination of adenosine₂-coralyne-adenosine₂.
abstract: A simple, sensitive, and selective-detection system for polyadenylation reaction was developed based on the fact that coralyne induces conformational change of polyadenosine [poly(A)] oligonucleotide through adenosine2-coralyne-adenosine2 coordination. Double-strand chelating dye SYBR Green I fluoresces weakly in the presence of random coil of poly(A). Coralyne enables poly(A) to change its conformation from a random coil to a folded structure, which leads to an increased fluorescence of SYBR Green I. When the 3' end of RNA is polyadenylated with poly(A) polymerase, coralyne-induced fluorescence enhancement of the poly(A)-SYBR Green I complex is well suited for monitoring polyadenylation reaction.
authors.affiliation: Department of Chemistry, National Sun Yat-sen University, Kaohsiung, Taiwan.
authors.forename: Jia-Hui
authors.initials: JH
authors.lastname: Lin
authors.forename: Wei-Lung
authors.initials: WL
authors.lastname: Tseng
mesh_terms: D000241:Adenosine / Q000378:metabolism*; D026723:Polyadenylation / Q000502:physiology*; D011062:Polynucleotide Adenylyltransferase / Q000378:metabolism; D012313:RNA / Q000378:metabolism
chemical_list: D012313:RNA; D011062:Polynucleotide Adenylyltransferase; D000241:Adenosine