title: An antilisterial bacteriocin BacFL31 produced by Enterococcus faecium FL31 with a novel structure containing hydroxyproline residues.
abstract: A new bacterium called FL31, which was selected for its antimicrobial activity against the pathogenic bacterium Listeria monocytogenes, was identified as Enterococcus faecium and noted to produce an antibacterial proteinaceous substance (BacFL31). The active peptide from the cell-free supernatant of E. faecium FL31 was purified in four steps and the results revealed a single band with an estimated molecular mass of approximately 3.5 kDa. The N-terminal amino acid sequence of the purified BacFL31 "GLEESXGHXGQXGPXGPXGAXGP" (X = hydroxyproline) showed the presence of six hydroxyproline residues. It displayed a bactericidal mode of action against L. monocytogenes. Its application at 400 AU/g was also noted to constitute an effective approach for preventing the contamination and growth of the pathogenic bacterium L. monocytogenes during the storage of minced beef meat at 4 °C.
authors.affiliation: Laboratoire de Microorganismes et de Biomolécules du Centre de Biotechnologie de Sfax, Route de Sidi Mansour Km 6, B. P. 1177, 3018 Sfax, Tunisia.
authors.forename: Ahlem
authors.initials: A
authors.lastname: Chakchouk-Mtibaa
authors.affiliation: Laboratoire de Microorganismes et de Biomolécules du Centre de Biotechnologie de Sfax, Route de Sidi Mansour Km 6, B. P. 1177, 3018 Sfax, Tunisia.
authors.forename: Lobna
authors.initials: L
authors.lastname: Elleuch
authors.affiliation: Laboratoire de Microorganismes et de Biomolécules du Centre de Biotechnologie de Sfax, Route de Sidi Mansour Km 6, B. P. 1177, 3018 Sfax, Tunisia.
authors.forename: Slim
authors.initials: S
authors.lastname: Smaoui
authors.affiliation: Laboratoire de Microorganismes et de Biomolécules du Centre de Biotechnologie de Sfax, Route de Sidi Mansour Km 6, B. P. 1177, 3018 Sfax, Tunisia.
authors.forename: Soumaya
authors.initials: S
authors.lastname: Najah
authors.affiliation: Laboratoire de Microorganismes et de Biomolécules du Centre de Biotechnologie de Sfax, Route de Sidi Mansour Km 6, B. P. 1177, 3018 Sfax, Tunisia.
authors.forename: Imen
authors.initials: I
authors.lastname: Sellem
authors.affiliation: Département de Génie Biologique de l'Ecole Nationale d'Ingénieurs de Sfax, Route de Sokra Km 3,5, 3018 Sfax, Tunisia.
authors.forename: Slim
authors.initials: S
authors.lastname: Abdelkafi
authors.affiliation: Laboratoire de Microorganismes et de Biomolécules du Centre de Biotechnologie de Sfax, Route de Sidi Mansour Km 6, B. P. 1177, 3018 Sfax, Tunisia. Electronic address: lotfi.mallouli@cbs.rnrt.tn.
authors.forename: Lotfi
authors.initials: L
authors.lastname: Mellouli
mesh_terms: D000595:Amino Acid Sequence; D001430:Bacteriocins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D016984:Enterococcus faecium / Q000378:metabolism*; D005519:Food Preservation / Q000379:methods; D006909:Hydroxyproline / Q000032:analysis*; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development; D050296:Microbial Viability / Q000187:drug effects; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D001430:Bacteriocins; D010455:Peptides; D006909:Hydroxyproline
keywords: BacFL31; Biocontrol; Enterococcus faecium FL31; Hydroxyproline; Listeria monocytogenes; Minced beef meat