title: Intra-peritoneal and intra-rectal immunogenicity induced by rotavirus virus like particles 2/6/7 in mice.
abstract: We previously developed virus like particles of rotavirus (RV) with VP2, VP6, and VP7 proteins (VLP2/6/7) using stable High-five cell line. To evaluate the immunogenicity of our construct, we assessed the humoral and cytokine responses induced by VLP2/6/7 in BALB/c mice immunized intra-peritoneally and intra-rectally. Enzyme-linked immunosorbent assay (ELISA) and Relative quantitative (RQ) Real-time PCR were used to evaluate the antibody (IgG and IgA) levels in serum and mRNA levels of IL-6, IL-10 and IFN-γ in spleen cells, respectively. Our results showed that VLP2/6/7 is capable of intra-peritoneal (I.P.) and intra-rectal (I.R.) induction of serum IgG and IgA responses. IgA was detected in fecal samples of immunization groups by I.P. and I.R. routes. Interestingly, I.R. route induced higher IgA titer compared with I.P. route which was statistically significant. Moreover, mRNA levels of IL-6 and IFN-γ were significantly elevated in mice immunized intra-peritoneally with VLP2/6/7 compared to control group. As such, the mean change was 7.4 (P < 0.05) and 14.8 (P < 0.001) for IFN-γ and IL-6, respectively. Likewise, the same pattern was found when mice were immunized intra-rectally. Although elevated, the difference in the mean change for IL-10 was not statistically significant when compared to control group. Our findings indicated that VLPs constructed via a stable insect cell line are able to induce both humoral and cellular responses, a similar pattern as observed after immunization with live RVs.
authors.affiliation: Virology Department, School of Public Health, Tehran University of Medical Sciences, 14155 Tehran, Iran.
authors.forename: Sayed-Mahdi
authors.initials: SM
authors.lastname: Marashi
authors.affiliation: Virology Department, School of Public Health, Tehran University of Medical Sciences, 14155 Tehran, Iran.
authors.forename: Somayeh
authors.initials: S
authors.lastname: Jalilvand
authors.affiliation: Virology Department, School of Public Health, Tehran University of Medical Sciences, 14155 Tehran, Iran.
authors.forename: Yaghoub
authors.initials: Y
authors.lastname: Mollaei-Kandelous
authors.affiliation: Virology Department, School of Public Health, Tehran University of Medical Sciences, 14155 Tehran, Iran.
authors.forename: Shohreh
authors.initials: S
authors.lastname: Shahmahmoodi
authors.affiliation: Virology Department, School of Public Health, Tehran University of Medical Sciences, 14155 Tehran, Iran.
authors.forename: Farhad
authors.initials: F
authors.lastname: Rezaei
authors.affiliation: Virology Department, School of Public Health, Tehran University of Medical Sciences, 14155 Tehran, Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Salimi
authors.affiliation: Virology Department, School of Public Health, Tehran University of Medical Sciences, 14155 Tehran, Iran.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Nejati
authors.affiliation: Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Validi
authors.affiliation: Virology Department, School of Public Health, Tehran University of Medical Sciences, 14155 Tehran, Iran; Virology Department, Pasteur Institute of Iran, Tehran, Iran. Electronic address: zshoja@razi.tums.ac.ir.
authors.forename: Zabihollah
authors.initials: Z
authors.lastname: Shoja
mesh_terms: D000285:Administration, Rectal; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D036022:Capsid Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D005260:Female; D007114:Immunization; D007263:Infusions, Parenteral; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012401:Rotavirus / Q000235:genetics / Q000276:immunology*; D012400:Rotavirus Infections / Q000235:genetics / Q000276:immunology* / Q000821:virology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D015850:Interleukin-6; C442545:VP2 protein, Rotavirus; C061332:VP6 protein, Rotavirus; C050779:VP7 protein, Rotavirus; D014765:Viral Vaccines; D016753:Interleukin-10
keywords: Antibody; Cytokine; VLP2/6/7