title: In site bioimaging of hydrogen sulfide uncovers its pivotal role in regulating nitric oxide-induced lateral root formation.
abstract: Hydrogen sulfide (H2S) is an important gasotransmitter in mammals. Despite physiological changes induced by exogenous H2S donor NaHS to plants, whether and how H2S works as a true cellular signal in plants need to be examined. A self-developed specific fluorescent probe (WSP-1) was applied to track endogenous H2S in tomato (Solanum lycopersicum) roots in site. Bioimaging combined with pharmacological and biochemical approaches were used to investigate the cross-talk among H2S, nitric oxide (NO), and Ca(2+) in regulating lateral root formation. Endogenous H2S accumulation was clearly associated with primordium initiation and lateral root emergence. NO donor SNP stimulated the generation of endogenous H2S and the expression of the gene coding for the enzyme responsible for endogenous H2S synthesis. Scavenging H2S or inhibiting H2S synthesis partially blocked SNP-induced lateral root formation and the expression of lateral root-related genes. The stimulatory effect of SNP on Ca(2+) accumulation and CaM1 (calmodulin 1) expression could be abolished by inhibiting H2S synthesis. Ca(2+) chelator or Ca(2+) channel blocker attenuated NaHS-induced lateral root formation. Our study confirmed the role of H2S as a cellular signal in plants being a mediator between NO and Ca(2+) in regulating lateral root formation.
authors.affiliation: College of Life Sciences, Nanjing Agricultural University, Nanjing, China ; Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Li
authors.affiliation: Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Washington State University, Pullman, Washington, United States of America.
authors.forename: Ming
authors.initials: M
authors.lastname: Xian
authors.affiliation: Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing, China.
authors.forename: Li-Gang
authors.initials: LG
authors.lastname: Zhou
authors.affiliation: Department of Chemistry and Biochemistry, Jackson State University, Jackson, Mississippi, United States of America.
authors.forename: Fengxiang X
authors.initials: FX
authors.lastname: Han
authors.affiliation: College of Life Sciences, Nanjing Agricultural University, Nanjing, China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Gan
authors.affiliation: Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
authors.forename: Zhi-Qi
authors.initials: ZQ
authors.lastname: Shi
mesh_terms: D002118:Calcium / Q000378:metabolism; D002147:Calmodulin / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006862:Hydrogen Sulfide / Q000378:metabolism* / Q000494:pharmacology; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism; D057054:Molecular Imaging; D009569:Nitric Oxide / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D002147:Calmodulin; D009569:Nitric Oxide; D002118:Calcium; D006862:Hydrogen Sulfide