title: Expanding the genetic code for site-specific labelling of tobacco mosaic virus coat protein and building biotin-functionalized virus-like particles.
abstract: A method for site-specific and high yield modification of tobacco mosaic virus coat protein (TMVCP) utilizing a genetic code expanding technology and copper free cycloaddition reaction has been established, and biotin-functionalized virus-like particles were built by the self-assembly of the protein monomers.
authors.affiliation: Technical Institute of Physics and Chemistry, CAS, Beijing 100190, China. niu@mail.ipc.ac.cn.
authors.forename: F C
authors.initials: FC
authors.lastname: Wu
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.forename: Q
authors.initials: Q
authors.lastname: Zhou
authors.forename: M
authors.initials: M
authors.lastname: Wu
authors.forename: Z
authors.initials: Z
authors.lastname: Ballard
authors.forename: Y
authors.initials: Y
authors.lastname: Tian
authors.forename: J Y
authors.initials: JY
authors.lastname: Wang
authors.forename: Z W
authors.initials: ZW
authors.lastname: Niu
authors.forename: Y
authors.initials: Y
authors.lastname: Huang
mesh_terms: D001710:Biotin / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D015394:Molecular Structure; D013194:Staining and Labeling; D013499:Surface Properties; D014027:Tobacco Mosaic Virus / Q000235:genetics*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D014771:Virion / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D014764:Viral Proteins; D001710:Biotin