title: Small heat shock protein degradation could be an indicator of the extent of myofibrillar protein degradation.
abstract: The objective of the present study was to evaluate the relationship between small heat shock proteins (sHSP) degradation and tenderness development of beef loins at different ultimate pH (pHu). A total of twelve loins (M. longissimus dorsi) from steers were obtained at 1 day post mortem. Shear force and proteolysis of each loin were analyzed at 1 and 28 days post mortem. The loins at intermediate pHu (5.8 to 6.0) showed more variation in tenderness compared to the loins at low pHu (<5.8), where few samples were still tough (>10kgF) at 28days. The intact sHSP20 was more pronounced (P<0.05) in the intermediate pHu loin compared to the low pHu counterpart. Further, high correlations between the degradation of both sHSP and myofibrillar proteins were observed (e.g. r=0.94; degraded sHSP27 and degraded desmin). The result of this study suggests that the extent of sHSP degradation could be an indicator of myofibrillar protein degradation and tenderness.
authors.affiliation: AgResearch Ltd., Ruakura Research Centre, Hamilton, New Zealand.
authors.forename: Prabhu
authors.initials: P
authors.lastname: Balan
authors.affiliation: AgResearch Ltd., Ruakura Research Centre, Hamilton, New Zealand; Muscle Biology Lab, Department of Animal Sciences, Purdue University, West Lafayette, IN 47906, United States. Electronic address: bradkim@purdue.edu.
authors.forename: Yuan H Brad
authors.initials: YH
authors.lastname: Kim
authors.affiliation: AgResearch Ltd., Ruakura Research Centre, Hamilton, New Zealand; Hogeschool Inholland, University of Applied Sciences, Netherlands.
authors.forename: Rosanne
authors.initials: R
authors.lastname: Blijenburg
mesh_terms: D000818:Animals; D002417:Cattle; D003893:Desmin / Q000737:chemistry; D050888:Heat-Shock Proteins, Small / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008297:Male; D008460:Meat / Q000032:analysis*; D009124:Muscle Proteins / Q000737:chemistry*; D018482:Muscle, Skeletal / Q000737:chemistry*; D009210:Myofibrils / Q000737:chemistry; D059748:Proteolysis; D013314:Stress, Mechanical
chemical_list: D003893:Desmin; D050888:Heat-Shock Proteins, Small; D009124:Muscle Proteins
keywords: Beef; Proteolysis; Small heat shock proteins; Tenderness