title: Rho-kinase levels in testicular ischemia-reperfusion injury and effects of its inhibitor, Y-27632, on oxidative stress, spermatogenesis, and apoptosis.
abstract: OBJECTIVE
  To investigate testicular Rho-kinase levels and the effects of its inhibitor, Y-27632, on oxidative stress, spermatogenesis, and apoptosis in testicular ischemia-reperfusion rat model.
  
  
  METHODS
  The study included 29 adult Wistar-Albino male rats weighing 150-200 g. The rats were divided into 3 groups. Group 1 underwent sham operation (n = 10). In group 2, left testicular torsion-detorsion was performed (n = 9). In group 3, Rho-kinase inhibitor Y-27632 (5 mg/kg) was injected intraperitoneally 30 minutes before detorsion (n = 10). Two months later, bilateral orchiectomy was performed in all the groups. Rho-kinase levels by Western blotting, apoptosis with terminal deoxynucleotidyl transferase dUTP nick end labeling method, testicular damage and spermatogenesis with modified Johnsen score, testicular total antioxidative status, and total oxidative status were measured.
  
  
  RESULTS
  In the torsion-detorsion (T/D) group, Rho-kinase level increased significantly, compared with the sham group (P = .025). In the Y-27632 treatment group, Johnsen scores were significantly higher, and apoptosis indexes were significantly lower, compared with the T/D group (P = .001). Significantly higher total antioxidative status levels and lower total oxidative status levels were observed in the Y-27632 treatment group, compared with the T/D group (P = .001 and P = .002, respectively).
  
  
  CONCLUSIONS
  Testicular ischemia-reperfusion significantly increased Rho-kinase levels in rats, and administration of Rho-kinase inhibitor, Y-27632, before detorsion might prevent ischemia-reperfusion injury.
authors.affiliation: Department of Urology, University of Mersin School of Medicine, Mersin, Turkey. Electronic address: selcayan@mersin.edu.tr.
authors.forename: Selahittin
authors.initials: S
authors.lastname: Çayan
authors.affiliation: Department of Urology, University of Mersin School of Medicine, Mersin, Turkey.
authors.forename: Barış
authors.initials: B
authors.lastname: Saylam
authors.affiliation: Department of Pharmacology, University of Mersin School of Medicine, Mersin, Turkey.
authors.forename: Nalan
authors.initials: N
authors.lastname: Tiftik
authors.affiliation: Department of Biochemistry, University of Mersin School of Medicine, Mersin, Turkey.
authors.forename: Nil Doğruer
authors.initials: ND
authors.lastname: Ünal
authors.affiliation: Department of Pathology, University of Mersin School of Medicine, Mersin, Turkey.
authors.forename: Duygu Düşmez
authors.initials: DD
authors.lastname: Apa
authors.affiliation: Department of Urology, University of Mersin School of Medicine, Mersin, Turkey.
authors.forename: Ozan
authors.initials: O
authors.lastname: Efesoy
authors.affiliation: Department of Biochemistry, University of Mersin School of Medicine, Mersin, Turkey.
authors.forename: Burak
authors.initials: B
authors.lastname: Çimen
authors.affiliation: Department of Urology, University of Mersin School of Medicine, Mersin, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Bozlu
authors.affiliation: Department of Urology, University of Mersin School of Medicine, Mersin, Turkey.
authors.forename: Erdem
authors.initials: E
authors.lastname: Akbay
authors.affiliation: Department of Pharmacology, University of Mersin School of Medicine, Mersin, Turkey.
authors.forename: Kansu
authors.initials: K
authors.lastname: Büyükafşar
mesh_terms: D000577:Amides / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D011725:Pyridines / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000201:enzymology* / Q000209:etiology / Q000503:physiopathology; D013086:Spermatic Cord Torsion / Q000150:complications / Q000503:physiopathology; D013091:Spermatogenesis / Q000187:drug effects; D013737:Testis / Q000098:blood supply / Q000201:enzymology* / Q000473:pathology; D054460:rho-Associated Kinases / Q000378:metabolism*
chemical_list: D000577:Amides; D000975:Antioxidants; D004791:Enzyme Inhibitors; D011725:Pyridines; C108830:Y 27632; D054460:rho-Associated Kinases