title: Lipid-polymer nanoparticles encapsulating curcumin for modulating the vascular deposition of breast cancer cells.
abstract: Vascular adhesion and endothelial transmigration are critical steps in the establishment of distant metastasis by circulating tumor cells (CTCs). Also, vascular inflammation plays a pivotal role in steering CTCs out of the blood stream. Here, long circulating lipid-polymer nanoparticles encapsulating curcumin (NANOCurc) are proposed for modulating the vascular deposition of CTCs. Upon treatment with NANOCurc, the adhesion propensity of highly metastatic breast cancer cells (MDA-MB-231) onto TNF-α stimulated endothelial cells (HUVECs) reduces by ~70%, in a capillary flow. Remarkably, the CTCs vascular deposition already reduces up to ~50% by treating solely the inflamed HUVECs. The CTCs arrest is mediated by the interaction between ICAM-1 on HUVECs and MUC-1 on cancer cells, and moderate doses of curcumin down-regulate the expression of both molecules. This suggests that NANOCurc could prevent metastasis and limit the progression of the disease by modulating vascular inflammation and impairing the CTCs arrest.
  
  
  BACKGROUND
  In this novel study, lipid nanoparticles encapsulating curcumin were able to prevent metastasis formation and limited the progression of the disease by modulating vascular inflammation and impairing the circulating tumor cells' arrest as a result of down-regulation of ICAM1 and MUC1 in a highly metastatic breast cancer cell line model.
authors.affiliation: Department of Translational Imaging and Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX, USA; Department of Experimental and Clinical Medicine, University of Magna Graecia, Catanzaro, Italy.
authors.forename: Anna L
authors.initials: AL
authors.lastname: Palange
authors.affiliation: Department of Translational Imaging and Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX, USA; Department of Experimental and Clinical Medicine, University of Magna Graecia, Catanzaro, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Di Mascolo
authors.affiliation: Department of Experimental and Clinical Medicine, University of Magna Graecia, Catanzaro, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Carallo
authors.affiliation: Department of Experimental and Clinical Medicine, University of Magna Graecia, Catanzaro, Italy.
authors.forename: Agostino
authors.initials: A
authors.lastname: Gnasso
authors.affiliation: Department of Translational Imaging and Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX, USA; Department of Experimental and Clinical Medicine, University of Magna Graecia, Catanzaro, Italy. Electronic address: pdecuzzi@houstonmethodist.org.
authors.forename: Paolo
authors.initials: P
authors.lastname: Decuzzi
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D003474:Curcumin / Q000737:chemistry* / Q000494:pharmacology*; D005260:Female; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008055:Lipids / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D008055:Lipids; D011108:Polymers; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D003474:Curcumin
keywords: Cancer prevention; Cell adhesion; Metastasis; Nanoparticles; Vascular inflammation