title: Stromal vascular fraction improves deep partial thickness burn wound healing.
abstract: OBJECTIVE
  The practice of early burn wound excision and wound closure by immediate autologous skin or skin substitutes is the preferred treatment in extensive deep partial and full-thickness burns. To date there is no proven definite medical treatment to decrease burn wound size and accelerate burn wound healing in modern clinical practice. Stromal vascular fraction is an autologous mixture that has multiple proven beneficial effects on different kinds of wounds. In our study, we investigated the effects of stromal vascular fraction on deep partial-thickness burn wound healing.
  
  
  METHODS
  In this study, 20 Wistar albino rats were used. Inguinal adipose tissue of the rats was surgically removed and stromal vascular fraction was isolated. Thereafter, deep second-degree burns were performed on the back of the rats by hot water. The rats were divided into two groups in a randomized fashion. The therapy group received stromal vascular fraction, whereas the control group received only physiologic serum by intradermal injection. Assessment of the burn wound healing between the groups was carried out by histopathologic and immuno-histochemical data.
  
  
  RESULTS
  Stromal vascular fraction increased vascular endothelial growth factor, proliferating cell nuclear antigen index, and reduced inflammation of the burn wound. Furthermore, vascularization and fibroblastic activity were achieved earlier and observed to be at higher levels in the stromal vascular fraction group.
  
  
  CONCLUSIONS
  Stromal vascular fraction improves burn wound healing by increasing cell proliferation and vascularization, reducing inflammation, and increasing fibroblastic activity.
authors.affiliation: Private Medisu Hospital, Department of Plastic Reconstructive and Aesthetic Surgery, Antalya, Turkey.
authors.forename: Sibel
authors.initials: S
authors.lastname: Atalay
authors.affiliation: Erciyes University Medical Faculty, Department of Plastic Aesthetic and Reconstructive Surgery , Kayseri, Turkey. Electronic address: atilla.coruh@superonline.com.
authors.forename: Atilla
authors.initials: A
authors.lastname: Coruh
authors.affiliation: Erciyes University Medical Faculty, Department of Pathology, Kayseri, Turkey.
authors.forename: Kemal
authors.initials: K
authors.lastname: Deniz
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002056:Burns / Q000378:metabolism / Q000628:therapy*; D049109:Cell Proliferation; D005347:Fibroblasts / Q000378:metabolism*; D006119:Groin; D007249:Inflammation; D018919:Neovascularization, Physiologic; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D044967:Serum; D033581:Stem Cell Transplantation; D017154:Stromal Cells / Q000637:transplantation*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat
keywords: Adipose tissue derived stem cell; Burn wound; Burn wound healing; Deep partial thickness burn; Deep second degree burn; Stromal vascular fraction; stem cell