title: Identification and molecular characterization of a novel Chlamydomonas reinhardtii mutant defective in chlorophyll biosynthesis.
abstract: The green micro-alga Chlamydomonas reinhardtii is an elegant model organism to study all aspects of oxygenic photosynthesis. Chlorophyll (Chl) and heme are major tetrapyrroles that play an essential role in energy metabolism in photosynthetic organisms and are synthesized via a common branched tetrapyrrole biosynthetic pathway. One of the enzymes in the pathway is Mg chelatase (MgChel) which inserts Mg (2+) into protoporphyrin IX (PPIX, proto) to form magnesium-protoporphyrin IX (MgPPIX, Mgproto), the first biosynthetic intermediate in the Chl branch. MgChel is a multimeric enzyme that consists of three subunits designated CHLD, CHLI and CHLH. Plants have two isozymes of CHLI (CHLI1 and CHLI2) which are 70%-81% identical in protein sequences. Although the functional role of CHLI1 is well characterized, that of CHLI2 is not. We have isolated a non-photosynthetic light sensitive mutant 5A7 by random DNA insertional mutagenesis that is devoid of any detectable Chl. PCR based analyses show that 5A7 is missing the CHLI1 gene and at least eight additional functionally uncharacterized genes. 5A7 has an intact CHLI2 gene. Complementation with a functional copy of the CHLI1 gene restored Chl biosynthesis, photo-autotrophic growth and light tolerance in 5A7. We have identified the first chli1 (chli1-1) mutant of Chlamydomonas reinhardtii and in green algae. Our results show that in the wild type Chlamydomonas CHLI2 protein amount is lower than that of CHLI1 and the chli1-1 mutant has a drastic reduction in CHLI2 protein levels although it possesses the CHLI2 gene. Our chli1-1 mutant opens up new avenues to explore the functional roles of CHLI1 and CHLI2 in Chl biosynthesis in Chlamydomonas, which has never been studied before.
authors.affiliation: Department of Biology, University of West Georgia, Carrollton GA, 30118, USA.
authors.forename: Phillip B
authors.initials: PB
authors.lastname: Grovenstein
authors.affiliation: Department of Biology, University of West Georgia, Carrollton GA, 30118, USA.
authors.forename: Darryel A
authors.initials: DA
authors.lastname: Wilson
authors.affiliation: Department of Biology, University of West Georgia, Carrollton GA, 30118, USA.
authors.forename: Cameron G
authors.initials: CG
authors.lastname: Lennox
authors.affiliation: Department of Biology, University of West Georgia, Carrollton GA, 30118, USA.
authors.forename: Katherine P
authors.initials: KP
authors.lastname: Smith
authors.affiliation: Department of Biology, University of West Georgia, Carrollton GA, 30118, USA.
authors.forename: Alisha A
authors.initials: AA
authors.lastname: Contractor
authors.affiliation: Department of Biology, University of West Georgia, Carrollton GA, 30118, USA.
authors.forename: Jonathan L
authors.initials: JL
authors.lastname: Mincey
authors.affiliation: Department of Biology, University of West Georgia, Carrollton GA, 30118, USA.
authors.forename: Kathryn D
authors.initials: KD
authors.lastname: Lankford
authors.affiliation: Department of Biology, University of West Georgia, Carrollton GA, 30118, USA.
authors.forename: Jacqueline M
authors.initials: JM
authors.lastname: Smith
authors.affiliation: Department of Biology, University of West Georgia, Carrollton GA, 30118, USA.
authors.forename: Tashana C
authors.initials: TC
authors.lastname: Haye
authors.affiliation: Department of Biology, University of West Georgia, Carrollton GA, 30118, USA.
authors.forename: Mautusi
authors.initials: M
authors.lastname: Mitra