title: Carboxybetaine methacrylate-modified nylon surface for circulating tumor cell capture.
abstract: Conventional in vitro circulating tumor cell (CTC) detection methods are always limited by blood sample volume because of the requirement of a large amount of blood. The aim of this study was to overcome the limitation by designing and making an in vivo CTC capture device. In this study, we designed and prepared a kind of proper material to serve the purpose of intervention. A method employing 3-aminopropyltriethoxysilane (γ-APS) as the coupling reagent to graft carboxybetaine methacrylate (CBMA) and to immobilize an anti-epithelial cell adhesion molecular (EpCAM) antibody on Nylon was developed. The results of X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy proved the successful graft of γ-APS and CBMA to Nylon. Furthermore, the predicted improvement in the biocompatibilities of our modified Nylon was confirmed by water contact angle measurement, bovine serum albumin adhesion, platelet adhesion, plasma recalcification time determination, and cytotoxicity tests. The tumor cells adhesion experiment revealed that Nylon with the antibody immobilized on it had an affinity for EpCAM positive tumor cells higher than that of pristine Nylon. Additionally, the capture ability of the CTCs was demonstrated in a nude mouse tumor model using the interventional device made of the modified Nylon wire. The positive results suggest that CBMA-grafted and anti-EpCAM antibody-immobilized Nylon is a promising new material for in vivo CTC capture devices.
authors.affiliation: Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University , 321 Zhongshan Road, Nanjing 210008, China.
authors.forename: Huiyu
authors.initials: H
authors.lastname: Wang
authors.forename: Guofeng
authors.initials: G
authors.lastname: Yue
authors.forename: Chaoqun
authors.initials: C
authors.lastname: Dong
authors.forename: Fenglei
authors.initials: F
authors.lastname: Wu
authors.forename: Jia
authors.initials: J
authors.lastname: Wei
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Zhengyun
authors.initials: Z
authors.lastname: Zou
authors.forename: Lifeng
authors.initials: L
authors.lastname: Wang
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Qian
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Baorui
authors.initials: B
authors.lastname: Liu
mesh_terms: D000818:Animals; D001622:Betaine / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D002448:Cell Adhesion; D006801:Humans; D008689:Methacrylates / Q000737:chemistry*; D051379:Mice; D008819:Mice, Nude; D009369:Neoplasms / Q000737:chemistry / Q000175:diagnosis*; D009360:Neoplastic Cells, Circulating / Q000737:chemistry*; D009757:Nylons / Q000737:chemistry*
chemical_list: D008689:Methacrylates; D009757:Nylons; D001622:Betaine