title: Is there a relation between triglyceride concentrations in very low density lipoprotein and the index of insulin resistance in nondiabetic subjects?
abstract: BACKGROUND
  Serum very low density lipoprotein (VLDL) levels increase during the early stages of insulin resistance; therefore, determination of VLDL levels would be useful for evaluating the progression of metabolic syndrome and diabetes mellitus. The aim of this study was to clarify the clinical utility of triglyceride in VLDL (VLDL-TG) level, determined using a homogeneous assay kit (Shino-test Corporation, Tokyo, Japan), as an index of insulin resistance.
  
  
  METHODS
  We enrolled 74 subjects in this study (diabetic subjects, n = 42; nondiabetic subjects, n = 32). The levels of VLDL-TG, remnant-like lipoprotein particle cholesterol, preheparin lipoprotein lipase mass, and other biochemical markers were determined.
  
  
  RESULTS
  VLDL-TG levels were significantly higher in the diabetic group (1.04 ± 0.84 mmol/l vs. 0.64 ± 0.42 mmol/l, P < 0.01) than in the nondiabetic group. In the nondiabetic group, VLDL-TG was significantly correlated with the homeostasis model assessment of insulin resistance (HOMA-IR), the index for insulin resistance (r = 0.513, P = 0.003). VLDL-TG levels, but not TG levels, were higher in the highest quartile (HOMA-IR) of the nondiabetic group.
  
  
  CONCLUSIONS
  VLDL-TG level was a useful early marker for insulin resistance, especially in nondiabetic subjects. The homogeneous VLDL-TG assay is a simple, low-cost method for determining insulin resistance.
authors.affiliation: Department of Molecular Medical Biology, Graduate School of Medical Sciences, Kitasato University, Sagamihara, Kanagawa, Japan; Department of Clinical Chemistry, School of Allied Health Sciences, Kitasato University, Sagamihara, Kanagawa, Japan.
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Kurosaki
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Tsukushi
authors.forename: Shinichi
authors.initials: S
authors.lastname: Munekata
authors.forename: Yuhsaku
authors.initials: Y
authors.lastname: Kanoh
authors.forename: Tatsumi
authors.initials: T
authors.lastname: Moriya
authors.forename: Makoto
authors.initials: M
authors.lastname: Nishinari
authors.forename: Naoyoshi
authors.initials: N
authors.lastname: Aoyama
authors.forename: Zensuke
authors.initials: Z
authors.lastname: Ogawa
mesh_terms: D003920:Diabetes Mellitus / Q000097:blood*; D005260:Female; D006706:Homeostasis; D006801:Humans; D007333:Insulin Resistance; D008079:Lipoproteins, VLDL / Q000097:blood*; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D014280:Triglycerides / Q000097:blood*
chemical_list: D008079:Lipoproteins, VLDL; D014280:Triglycerides
keywords: HOMA-IR; VLDL-TG; diabetes; homogeneous kit; insulin resistance