title: Exploring DNA binding and nucleolytic activity of few 4-aminoantipyrine based amino acid Schiff base complexes: a comparative approach.
abstract: A series of novel Co(II), Cu(II), Ni(II) and Zn(II) complexes were synthesized from Schiff base(s), obtained by the condensation of 4-aminoantipyrine with furfural and amino acid (glycine(L1)/alanine(L2)/valine(L3)) and respective metal(II) chloride. Their structural features and other properties were explored from the analytical and spectral methods. The binding behaviors of the complexes to calf thymus DNA were investigated by absorption spectra, viscosity measurements and cyclic voltammetry. The intrinsic binding constants for the above synthesized complexes are found to be in the order of 10(2) to 10(5) indicating that most of the synthesized complexes are good intercalators. The binding constant values (Kb) clearly indicate that valine Schiff-base complexes have more intercalating ability than alanine and glycine Schiff-base complexes. The results indicate that the complexes bind to DNA through intercalation and act as efficient cleaving agents. The in vitro antibacterial and antifungal assay indicates that these complexes are good antimicrobial agents against various pathogens. The IC50 values of [Ni(L1)2] and [Zn(L1)2] complexes imply that these complexes have preferable ability to scavenge hydroxyl radical.
authors.affiliation: Research Department of Chemistry, VHNSN College, Virudhunagar 626 001, Tamil Nadu, India. Electronic address: ramchem1964@gmail.com.
authors.forename: N
authors.initials: N
authors.lastname: Raman
authors.affiliation: Research Department of Chemistry, VHNSN College, Virudhunagar 626 001, Tamil Nadu, India.
authors.forename: A
authors.initials: A
authors.lastname: Sakthivel
authors.affiliation: Research Department of Chemistry, VHNSN College, Virudhunagar 626 001, Tamil Nadu, India.
authors.forename: N
authors.initials: N
authors.lastname: Pravin
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism*; D000675:Ampyrone / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D002417:Cattle; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry; D004247:DNA / Q000378:metabolism*; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D004553:Electric Conductivity; D055664:Electrochemical Techniques; D004578:Electron Spin Resonance Spectroscopy; D004583:Electrons; D005658:Fungi / Q000187:drug effects; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D012545:Schiff Bases / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D012996:Solutions; D013055:Spectrophotometry, Infrared; D014783:Viscosity
chemical_list: D000596:Amino Acids; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D000975:Antioxidants; D056831:Coordination Complexes; D008024:Ligands; D012545:Schiff Bases; D012996:Solutions; D000675:Ampyrone; D004247:DNA; C427203:calf thymus DNA; D004121:Dimethyl Sulfoxide
keywords: 4-Aminoantipyrine; Antimicrobial studies; Calf thymus DNA; Intercalative mode; Schiff-base complexes