title: Neurobiological effect of 7-nitroindazole, a neuronal nitric oxide synthase inhibitor, in experimental paradigm of Alzheimer's disease.
abstract: Nitric oxide plays a role in a series of neurobiological functions, underlying behaviour and memory. The functional role of nNOS derived nitric oxide in cognitive functions is elusive. The present study was designed to investigate the effect of specific neuronal nitric oxide synthase inhibitor, 7-nitroindazole, against intracerebroventricular streptozotocin-induced cognitive impairment in rats. Learning and memory behaviour was assessed using Morris water maze and elevated plus maze. 7-nitroindazole (25 mg/kg, ip) was administered as prophylactically (30 min before intracerebroventricular streptozotocin injection on day 1) and therapeutically (30 min before the assessment of memory by Morris water maze on day 15). Intracerebroventricular streptozotocin produced significant cognitive deficits coupled with alterations in biochemical indices.These behavioural and biochemical changes were significantly prevented by prophylactic treatment of 7-nitroindazole. However, therapeutic intervention of 7-nitroindazole did not show any significant reversal. The results suggests that 7-nitroindazole can be effective in the protection of dementiainduced by intracerebroventricular streptozotocin only when given prophylactically but not therapeutically.
authors.affiliation: Pharmacology Research Laboratory, University Institute of Pharmaceutical Sciences UGC Center of Advanced Study, Panjab University, Chandigarh 160 014, India.
authors.forename: Shubham
authors.initials: S
authors.lastname: Misra
authors.affiliation: Pharmacology Research Laboratory, University Institute of Pharmaceutical Sciences UGC Center of Advanced Study, Panjab University, Chandigarh 160 014, India.
authors.forename: Anurag
authors.initials: A
authors.lastname: Kuhad
authors.affiliation: Pharmacology Research Laboratory, University Institute of Pharmaceutical Sciences UGC Center of Advanced Study, Panjab University, Chandigarh 160 014, India.
authors.forename: Kanwaljit
authors.initials: K
authors.lastname: Chopra
mesh_terms: D000544:Alzheimer Disease / Q000139:chemically induced / Q000201:enzymology* / Q000473:pathology; D000818:Animals; D003072:Cognition Disorders / Q000139:chemically induced / Q000201:enzymology / Q000473:pathology; D004791:Enzyme Inhibitors / Q000008:administration & dosage; D006801:Humans; D007191:Indazoles / Q000008:administration & dosage*; D008297:Male; D018782:Maze Learning / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D009569:Nitric Oxide / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D013311:Streptozocin / Q000633:toxicity
chemical_list: D004791:Enzyme Inhibitors; D007191:Indazoles; D009569:Nitric Oxide; D013311:Streptozocin; D052248:Nitric Oxide Synthase Type I; C080122:7-nitroindazole