title: [Establishment of a detection method for hepatitis B virus large surface protein (HBV-lP)].
abstract: OBJECTIVE
  To establish enzyme-linked immunosorbent assay (ELISA) for detection of hepatitis B virus large surface protein(HBV-LP) in serum.
  
  
  METHODS
  A sandwich reaction was preformed with horseradish peroxidase labeled monoclonal antibody of HBV-LP as the catalytic enzyme. Several reactions liquid's concentration and reaction conditions were optimized. The method was evaluated in all aspects such as sensitivity, specificity, stability and so on.
  
  
  RESULTS
  The detection limit was 5 ng/ml. Interassay and intra-assay RSD were both less than 10%. After stored at 4 degrees C and 37 degrees C for 3, 5, 7 days, the analysis showed correlation coefficient higher than 0.98 and RSD lower than 10%.
  
  
  CONCLUSIONS
  Established ELISA for determination of serum HBV-LP has high sensitivity and repeatability. Enzyme-linked immunosorbent assay;
authors.affiliation: Center of Clinical Laboratory, the No. 302 Hospital of the PLA, Beijing 100039, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Center of Clinical Laboratory, the No. 302 Hospital of the PLA, Beijing 100039, China.
authors.forename: Hong-Bin
authors.initials: HB
authors.lastname: Ma
authors.affiliation: Center of Clinical Laboratory, the No. 302 Hospital of the PLA, Beijing 100039, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Hou
authors.affiliation: Center of Clinical Laboratory, the No. 302 Hospital of the PLA, Beijing 100039, China.
authors.forename: Jing-Xia
authors.initials: JX
authors.lastname: Guo
authors.affiliation: Center of Clinical Laboratory, the No. 302 Hospital of the PLA, Beijing 100039, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.affiliation: Center of Clinical Laboratory, the No. 302 Hospital of the PLA, Beijing 100039, China.
authors.forename: Yong-Ji
authors.initials: YJ
authors.lastname: Song
authors.affiliation: Center of Clinical Laboratory, the No. 302 Hospital of the PLA, Beijing 100039, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.affiliation: Center of Clinical Laboratory, the No. 302 Hospital of the PLA, Beijing 100039, China.
authors.forename: Ai-Xia
authors.initials: AX
authors.lastname: Liu
authors.affiliation: Center of Clinical Laboratory, the No. 302 Hospital of the PLA, Beijing 100039, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.affiliation: Center of Clinical Laboratory, the No. 302 Hospital of the PLA, Beijing 100039, China.
authors.forename: Hong-Shan
authors.initials: HS
authors.lastname: Wei
authors.affiliation: Center of Clinical Laboratory, the No. 302 Hospital of the PLA, Beijing 100039, China.
authors.forename: Bo-An
authors.initials: BA
authors.lastname: Li
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006509:Hepatitis B / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006515:Hepatitis B virus / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans
chemical_list: D006514:Hepatitis B Surface Antigens