title: Novel mixed ligand di-n-butyltin(IV) complexes derived from acylpyrazolones and fluorinated benzoic acids: synthesis, characterization, cytotoxicity and the induction of apoptosis in Hela cancer cells.
abstract: Twenty one novel mixed ligand di-n-butyltin(IV) complexes [(n)Bu2SnAL] (A = substituted 4-acyl-5-pyrazolone, and L = fluorinated benzoic acid) were prepared by condensation of di-n-butyltin(IV) oxide with HL and HA in 1:1:1 molar ratio in refluxing methanol. All of the complexes were characterized by elemental analyses, IR, NMR ((1)H, (13)C, (119)Sn) and in four cases by X-ray diffraction. Cytotoxicity of the compounds was studied against two human cancer cell lines (KB and Hela) by means of the MTT assay compared to cisplatin, featuring IC₅₀ values in the low micromolar range. Hela cancer cell apoptosis-induced by 2 was examined by flow cytometry analysis, and preliminary results showed that 2 at concentrations of more than 1.0 μM can induce apoptosis.
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, PR China. Electronic address: shang430030@hotmail.com.
authors.forename: Xianmei
authors.initials: X
authors.lastname: Shang
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Xu
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, PR China.
authors.forename: Wendian
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, PR China. Electronic address: gyxiang1968@hotmail.com.
authors.forename: Guangya
authors.initials: G
authors.lastname: Xiang
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001565:Benzoates / Q000737:chemistry* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D005461:Fluorine / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D009947:Organotin Compounds / Q000737:chemistry* / Q000494:pharmacology; D047069:Pyrazolones / Q000737:chemistry* / Q000494:pharmacology; D014961:X-Ray Diffraction
chemical_list: D001565:Benzoates; D008024:Ligands; D009947:Organotin Compounds; D047069:Pyrazolones; C056189:di-n-butyltin; D005461:Fluorine
keywords: Apoptosis; Cytotoxicity; Dibutyltin; Mixed ligand; Synthesis