title: Immune response of broiler chickens immunized orally with the recombinant proteins flagellin and the subunit B of cholera toxin associated with Lactobacillus spp.
abstract: This study investigated the immune response of broiler chickens with oral treatment of a Lactobacillus spp. pool (PL) associated with microencapsulated recombinant proteins flagellin (FliC) and the subunit B of cholera toxin (CTB). Immune responses were evaluated by measuring IgA from intestinal fluid, serum IgY, and immunostaining of CD8(+) T lymphocytes present in the cecum. The evaluations were performed on d 0, 7, 14, 21, and 28 posttreatment. A significant increase (P < 0.05) was observed in IgA levels in all immunized groups, especially 3 wk after immunization. Treatments 2 (recombinant CTB) and 3 (recombinant FliC+CTB) showed the highest concentrations. Similarly, serum concentrations IgY (μg/mL) increased along the experiment, and the means for treatments 2 and 3 showed significant differences (P < 0.05) compared with controls, reaching concentrations of 533 and 540 μg/mL, respectively. The number of CD8(+) T lymphocytes in all treatments greatly differed (P < 0.05) compared with the negative control at 21 d posttreatment. However, only treatment 2 (recombinant CTB), 4 (PL), and 5 (recombinant FliC+ recombinant CTB + PL) remained significantly (P < 0.05) different from the control at 28 d posttreatment. Thus, it is concluded that the microencapsulated recombinant proteins administered orally to broiler chickens are capable of stimulating humoral and cellular immune response, and the combinations of these antigens with Lactobacillus spp. can influence the population of CD8(+) T cells residing in the cecum.
authors.affiliation: College of Veterinary Medicine and Animal Science, São Paulo State University, Botucatu, Sao Paulo, Brazil 18618-970.
authors.forename: A A S
authors.initials: AA
authors.lastname: Baptista
authors.forename: T C
authors.initials: TC
authors.lastname: Donato
authors.forename: K C O D
authors.initials: KC
authors.lastname: Garcia
authors.forename: G A M
authors.initials: GA
authors.lastname: Gonçalves
authors.forename: M P
authors.initials: MP
authors.lastname: Coppola
authors.forename: A S
authors.initials: AS
authors.lastname: Okamoto
authors.forename: J L
authors.initials: JL
authors.lastname: Sequeira
authors.forename: R L
authors.initials: RL
authors.lastname: Andreatti Filho
mesh_terms: D000276:Adjuvants, Immunologic; D000284:Administration, Oral; D000818:Animals; D018414:CD8-Positive T-Lymphocytes; D002645:Chickens; D002771:Cholera / Q000517:prevention & control / Q000662:veterinary*; D002772:Cholera Toxin / Q000276:immunology*; D005408:Flagellin / Q000276:immunology*; D018928:Immunity, Mucosal / Q000276:immunology*; D007136:Immunoglobulins / Q000097:blood; D007778:Lactobacillus; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; C016512:IgY; D007136:Immunoglobulins; D011994:Recombinant Proteins; D005408:Flagellin; D002772:Cholera Toxin
keywords: CD8+ T lymphocyte; Lactobacillus species; immunoglobulin A; recombinant cholera toxin subunit B; recombinant flagellin