title: Chronic exposure to WIN55,212-2 affects more potently spatial learning and memory in adolescents than in adult rats via a negative action on dorsal hippocampal neurogenesis.
abstract: Several epidemiological studies show an increase in cannabis use among adolescents, especially in Morocco for being one of the major producers in the world. The neurobiological consequences of chronic cannabis use are still poorly understood. In addition, brain plasticity linked to ontogeny portrays adolescence as a period of vulnerability to the deleterious effects of drugs. The aim of this study was to investigate the behavioral neurogenic effects of chronic exposure to the cannabinoid agonist WIN55,212-2 during adolescence, by evaluating the emotional and cognitive performances, and the consequences on neurogenesis along the dorso-ventral axis of the hippocampus in adult rats. WIN55,212 was administered intraperitoneally (i.p.) once daily for 20 days to adolescent (27-30 PND) and adult Wistar rats (54-57 PND) at the dose of 1mg/kg. Following a 20 day washout period, emotional and cognitive functions were assessed by the Morris water maze test and the two-way active avoidance test. Twelve hours after, brains were removed and hippocampal neurogenesis was assessed using the doublecortin (DCX) as a marker for cell proliferation. Our results showed that chronic WIN55,212-2 treatment significantly increased thigmotaxis early in the training process whatever the age of treatment, induced spatial learning and memory deficits in adolescent but not adult rats in the Morris water maze test, while it had no significant effect in the active avoidance test during multitrial training in the shuttle box. In addition, the cognitive deficits assessed in adolescent rats were positively correlated to a decrease in the number of newly generated neurons in dorsal hippocampus. These data suggest that long term exposure to cannabinoids may affect more potently spatial learning and memory in adolescent compared to adult rats via a negative action on hippocampal plasticity.
authors.affiliation: Laboratory of Biochemistry and Neurosciences, University Hassan 1(er). Settat, Morocco. Electronic address: oualid.ab@gmail.com.
authors.forename: Oualid
authors.initials: O
authors.lastname: Abboussi
authors.affiliation: Department of Pharmacology, Faculty of Medicine and Pharmacy. Casablanca, Morocco.
authors.forename: Abdelouahhab
authors.initials: A
authors.lastname: Tazi
authors.affiliation: Memory and Behavioural Plasticity Group, University Caen Basse-Normandie, Caen, France.
authors.forename: Eleni
authors.initials: E
authors.lastname: Paizanis
authors.affiliation: Laboratory of Biochemistry and Neurosciences, University Hassan 1(er). Settat, Morocco.
authors.forename: Soumaya
authors.initials: S
authors.lastname: El Ganouni
mesh_terms: D000375:Aging / Q000523:psychology; D000818:Animals; D001007:Anxiety / Q000523:psychology; D001362:Avoidance Learning / Q000187:drug effects; D048588:Benzoxazines / Q000633:toxicity*; D002121:Calcium Channel Blockers / Q000633:toxicity*; D000089662:Doublecortin Protein; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development*; D008297:Male; D018782:Maze Learning / Q000187:drug effects*; D008568:Memory / Q000187:drug effects*; D009025:Morpholines / Q000633:toxicity*; D009281:Naphthalenes / Q000633:toxicity*; D055495:Neurogenesis / Q000187:drug effects*; D009473:Neuronal Plasticity / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
chemical_list: D048588:Benzoxazines; D002121:Calcium Channel Blockers; C000714848:Dcx protein, rat; D000089662:Doublecortin Protein; D009025:Morpholines; D009281:Naphthalenes; C070417:(3R)-((2,3-dihydro-5-methyl-3-((4-morpholinyl)methyl)pyrrolo-(1,2,3-de)-1,4-benzoxazin-6-yl)(1-naphthalenyl))methanone
keywords: Adolescence; Cannabinoids; Hippocampal neurogenesis; Morris water maze; Shuttle-box