title: Cell cycle-dependent regulation of Aurora kinase B mRNA by the Microprocessor complex.
abstract: Aurora kinase B regulates the segregation of chromosomes and the spindle checkpoint during mitosis. In this study, we showed that the Microprocessor complex, which is responsible for the processing of the primary transcripts during the generation of microRNAs, destabilizes the mRNA of Aurora kinase B in human cells. The Microprocessor-mediated cleavage kept Aurora kinase B at a low level and prevented premature entrance into mitosis. The cleavage was reduced during mitosis leading to the accumulation of Aurora kinase B mRNA and protein. In addition to Aurora kinase B mRNA, the processing of other primary transcripts of miRNAs were also decreased during mitosis. We found that the cleavage was dependent on an RNA helicase, DDX5, and the association of DDX5 and DDX17 with the Microprocessor was reduced during mitosis. Thus, we propose a novel mechanism by which the Microprocessor complex regulates stability of Aurora kinase B mRNA and cell cycle progression.
authors.affiliation: Department of Biomedical Sciences, Korea University College of Medicine, Seoul 136-705, Republic of Korea.
authors.forename: Eunsun
authors.initials: E
authors.lastname: Jung
authors.affiliation: Department of Bioscience & Biotechnology, Sejong University, Seoul 143-747, Republic of Korea.
authors.forename: Youngmo
authors.initials: Y
authors.lastname: Seong
authors.affiliation: Department of Internal Medicine, Korea University Guro Hospital, Seoul 152-703, Republic of Korea.
authors.forename: Jae Hong
authors.initials: JH
authors.lastname: Seo
authors.affiliation: Department of Bioscience & Biotechnology, Sejong University, Seoul 143-747, Republic of Korea. Electronic address: yngskwon@sejong.ac.kr.
authors.forename: Young-Soo
authors.initials: YS
authors.lastname: Kwon
authors.affiliation: Department of Biomedical Sciences, Korea University College of Medicine, Seoul 136-705, Republic of Korea. Electronic address: hoseoksong@korea.ac.kr.
authors.forename: Hoseok
authors.initials: H
authors.lastname: Song
mesh_terms: D000818:Animals; D064107:Aurora Kinase B / Q000235:genetics*; D001483:Base Sequence; D059447:Cell Cycle Checkpoints / Q000235:genetics* / Q000502:physiology*; D002478:Cells, Cultured; D053487:DEAD-box RNA Helicases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008954:Models, Biological; D046912:Multiprotein Complexes / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D009690:Nucleic Acid Conformation; D011506:Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D020871:RNA Stability; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016601:RNA-Binding Proteins; D043244:Ribonuclease III / Q000235:genetics / Q000378:metabolism*
chemical_list: C474043:DGCR8 protein, human; D046912:Multiprotein Complexes; D011506:Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C576921:AURKB protein, human; C576924:Aurkb protein, mouse; D064107:Aurora Kinase B; C517918:DROSHA protein, human; D043244:Ribonuclease III; C102004:DDX17 protein, human; C506749:Ddx5 protein, human; D053487:DEAD-box RNA Helicases
keywords: Aurora kinase B; Cell cycle; Mitosis; The Microprocessor complex