title: Determination of oxidative stress and cellular inflammation in patients with diabetic nephropathy and non-diabetic nephropathy being administered hemodialysis treatment due to chronic renal failure.
abstract: OBJECTIVE
  We aimed to evaluate oxidative stress [8-hydroxydeoxyguanosine (8-OHdG), malondialdehyde (MDA)] endothelial damage [asymmetric dimethylarginine (ADMA)] and markers of cellular inflammation [interleukin 6 (IL-6), tumor necrosis factor-alpha (TNF-α), neopterin (NP) and high-sensitivity C-reactive protein (hsCRP)] in patients with diabetic nephropathy (DN) and non-diabetic nephropathy who were being administered hemodialysis treatment because of chronic renal failure.
  
  
  METHODS
  In determining 8-OHdG, IL-6 and TNF-α levels, Enzyme-Linked Immuno-Sorbent Assay method was used. Serum MDA, ADMA and NP levels were determined by using high performance liquid chromatography (HPLC). And hs-CRP values were measured with nephelometric method.
  
  
  RESULTS
  Serum 8-OHdG and MDA levels were found statistically to have increased when compared with those of the control group in patients groups after dialysis. However, serum ADMA and neopterin levels were observed statistically to have decreased when compared with those of the control group in patients groups after dialysis. But, decreases on ADMA and neopterin levels are still much higher than those of control. IL-6 and TNF-α levels were found to have increased when compared with those of control group in patients groups before dialysis.
  
  
  CONCLUSIONS
  The oxidative stress in patients with DN, who were being treated with hemodialysis due to chronic renal failure, was higher than that of non-DN patients who were being treated with hemodialysis. In contrast with this, inflammation occurring in non-DN patients was found to have been higher than that of in patients with DN.
authors.affiliation: Department of Biology/Biochemistry, Faculty of Science and Arts, Hitit University , Corum , Turkey .
authors.forename: Emre
authors.initials: E
authors.lastname: Avci
authors.forename: Erdinc
authors.initials: E
authors.lastname: Cakir
authors.forename: Sule Coskun
authors.initials: SC
authors.lastname: Cevher
authors.forename: Halil
authors.initials: H
authors.lastname: Yaman
authors.forename: Mehmet
authors.initials: M
authors.lastname: Agilli
authors.forename: Cumhur
authors.initials: C
authors.lastname: Bilgi
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000368:Aged; D001120:Arginine / Q000031:analogs & derivatives / Q000097:blood; D015415:Biomarkers / Q000097:blood*; D002097:C-Reactive Protein / Q000378:metabolism; D016022:Case-Control Studies; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood; D003928:Diabetic Nephropathies / Q000097:blood* / Q000150:complications; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood* / Q000209:etiology; D015850:Interleukin-6 / Q000097:blood; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000150:complications; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D019798:Neopterin / Q000097:blood; D018384:Oxidative Stress; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D015415:Biomarkers; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; C018524:N,N-dimethylarginine; D019798:Neopterin; D000080242:8-Hydroxy-2'-Deoxyguanosine; D002097:C-Reactive Protein; D001120:Arginine; D003849:Deoxyguanosine
keywords: Cellular inflammation; chronic renal failure; diabetic nephropathy; hemodialysis; oxidative stress