title: Tissue factor pathway inhibitor-2 silencing promotes hepatocellular carcinoma cell invasion in vitro.
abstract: Hepatocellular carcinoma (HCC) is a leading cause of cancer-related death in the world and metastasis is an essential aspect of HCC progression. Tissue factor pathway inhibitor-2 (TFPI-2) has been implicated as a potential suppressor gene to regulate tumor invasion and metastasis. In this study, we silenced TFPI-2 in the HCC cell line MHCC97-L and evaluated the role of TFPI-2 in cell invasion and its impact on gene expression. We showed in this study that stable TFPI-2 downregulation in MHCC97-L cells resulted in increased cell adhesion and invasion. We also showed that mRNA and protein expression levels of MMP-1/3, CD44, and ICAM-1 were increased, while those of MMP-2/9 were not changed by TFPI-2 silencing. Furthermore, silencing of TFPI-2 caused increased Akt phosphorylation level and NF-κB transcription in MHCC97-L cells. In conclusion, this study confirms that TFPI-2 downregulation can contribute to tumor invasion of HCC cells through alteration in the expression of metastasis-related genes.
authors.affiliation: Department of Infectious Diseases, First Affiliated Hospital of Xinxiang Medical University, Xinxiang, 453100, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhu
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Zeng
authors.forename: Zhen
authors.initials: Z
authors.lastname: Huang
authors.forename: Ting-Fang
authors.initials: TF
authors.lastname: Dong
authors.forename: Yong-Kun
authors.initials: YK
authors.lastname: Gui
authors.forename: Guang-Wen
authors.initials: GW
authors.lastname: Zhang
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D015536:Down-Regulation / Q000187:drug effects; D020868:Gene Silencing / Q000187:drug effects; D006023:Glycoproteins / Q000037:antagonists & inhibitors* / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D016328:NF-kappa B / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000473:pathology; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D034741:RNA, Small Interfering / Q000494:pharmacology
chemical_list: D006023:Glycoproteins; D016328:NF-kappa B; D034741:RNA, Small Interfering; C021240:tissue-factor-pathway inhibitor 2; D018799:Intercellular Adhesion Molecule-1; D051057:Proto-Oncogene Proteins c-akt
keywords: hepatocellular carcinoma; invasion; tissue factor pathway inhibitor-2