title: In vivo delivery of lenti-Cre or adeno-Cre into mice using intranasal instillation.
abstract: Lung cancer remains the leading cause of cancer deaths among both men and women, with a lower rate of survival than both breast and prostate cancer. Development of the Cre/lox system and improved mouse models have allowed researchers to gain a better understanding of human disease, including lung cancer. Through the viral delivery of Cre, gene function in adult mice can be precisely studied at a specific developmental stage or in a specific cell/tissue type of choice. This protocol describes how to produce adenovirus-Cre precipitate. Using this adeno-Cre (or lentivirus-Cre), Cre can be expressed in mouse lungs. The virus is delivered by intranasal instillation.
authors.affiliation: Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Department of Pathology, Harvard Medical School, Charlestown, Massachusetts 02129;
authors.forename: Jessica J
authors.initials: JJ
authors.lastname: Gierut
authors.forename: Tyler E
authors.initials: TE
authors.lastname: Jacks
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Haigis
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000281:Administration, Intranasal; D000818:Animals; D005822:Genetic Vectors / Q000008:administration & dosage*; D019426:Integrases / Q000235:genetics; D016086:Lentivirus / Q000235:genetics*; D051379:Mice
chemical_list: C045073:Cre recombinase; D019426:Integrases