title: Detection of uridylated mRNAs.
abstract: Uridine addition at the 3' end of RNAs (i.e., uridylation) emerges as a critical posttranscriptional modification promoting RNA degradation. Uridylation has been notably linked to the degradation of small RNAs, correlated with the 5' shortening of RISC-cleaved transcripts and the degradation of mRNAs. We describe here a method based on 3' RACE (3' Rapid Amplification of cDNA End) PCR that has been successfully used to investigate nucleotide addition at the 3' end of RISC-cleaved transcripts and full-length mRNAs in plants.
authors.affiliation: Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg, Strasbourg Cedex, France.
authors.forename: François M
authors.initials: FM
authors.lastname: Sement
authors.forename: Dominique
authors.initials: D
authors.lastname: Gagliardi
mesh_terms: D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D018749:RNA, Plant / Q000737:chemistry / Q000235:genetics; D014529:Uridine / Q000737:chemistry*
chemical_list: D012333:RNA, Messenger; D018749:RNA, Plant; D014529:Uridine