title: Validation of 58 autosomal individual identification SNPs in three Chinese populations.
abstract: OBJECTIVE
  To genotype and evaluate a panel of single-nucleotide polymorphisms for individual identification (IISNPs) in three Chinese populations: Chinese Han, Uyghur, and Tibetan.
  
  
  METHODS
  Two previously identified panels of IISNPs, 86 unlinked IISNPs and SNPforID 52-plex markers, were pooled and analyzed. Four SNPs were included in both panels. In total, 132 SNPs were typed on Sequenom MassARRAY® platform in 330 individuals from Han Chinese, Uyghur, and Tibetan populations. Population genetic indices and forensic parameters were determined for all studied markers.
  
  
  RESULTS
  No significant deviation from Hardy-Weinberg equilibrium was observed for any of the SNPs in 3 populations. Expected heterozygosity (He) ranged from 0.144 to 0.500 in Han Chinese, from 0.197 to 0.500 in Uyghur, and from 0.018 to 0.500 in Tibetan population. Wright's Fst values ranged from 0.0001 to 0.1613. Pairwise linkage disequilibrium (LD) calculations for all 132 SNPs showed no significant LD across the populations (r(2)<0.147). A subset of 58 unlinked IISNPs (r(2)<0.094) with He>0.450 and Fst values from 0.0002 to 0.0536 gave match probabilities of 10-25 and a cumulative probability of exclusion of 0.999992.
  
  
  CONCLUSIONS
  The 58 unlinked IISNPs with high heterozygosity have low allele frequency variation among 3 Chinese populations, which makes them excellent candidates for the development of multiplex assays for individual identification and paternity testing.
authors.forename: Yi-Liang
authors.initials: YL
authors.lastname: Wei
authors.forename: Cui-Jiao
authors.initials: CJ
authors.lastname: Qin
authors.forename: Hai-Bo
authors.initials: HB
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Jia
authors.forename: Lan
authors.initials: L
authors.lastname: Hu
authors.affiliation: Cai-Xia Li, Institute of Forensic Science, Ministry of Public Security, Muxidi Nanli 17, Xicheng District, Beijing 100038, China, licaixia@tsinghua.org.cn.
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: Li
mesh_terms: D044466:Asian People / Q000235:genetics*; D002681:China; D005006:Ethnicity / Q000235:genetics*; D005787:Gene Frequency; D005819:Genetic Markers; D005820:Genetic Testing / Q000379:methods; D005828:Genetics, Population / Q000379:methods*; D005838:Genotype; D006801:Humans; D020641:Polymorphism, Single Nucleotide
chemical_list: D005819:Genetic Markers