title: Immune cell trafficking from the brain maintains CNS immune tolerance.
abstract: In the CNS, no pathway dedicated to immune surveillance has been characterized for preventing the anti-CNS immune responses that develop in autoimmune neuroinflammatory disease. Here, we identified a pathway for immune cells to traffic from the brain that is associated with the rostral migratory stream (RMS), which is a forebrain source of newly generated neurons. Evaluation of fluorescently labeled leukocyte migration in mice revealed that DCs travel via the RMS from the CNS to the cervical LNs (CxLNs), where they present antigen to T cells. Pharmacologic interruption of immune cell traffic with the mononuclear cell-sequestering drug fingolimod influenced anti-CNS T cell responses in the CxLNs and modulated experimental autoimmune encephalomyelitis (EAE) severity in a mouse model of multiple sclerosis (MS). Fingolimod treatment also induced EAE in a disease-resistant transgenic mouse strain by altering DC-mediated Treg functions in CxLNs and disrupting CNS immune tolerance. These data describe an immune cell pathway that originates in the CNS and is capable of dampening anti-CNS immune responses in the periphery. Furthermore, these data provide insight into how fingolimod treatment might exacerbate CNS neuroinflammation in some cases and suggest that focal therapeutic interventions, outside the CNS have the potential to selectively modify anti-CNS immunity.
authors.forename: Mohammad G
authors.initials: MG
authors.lastname: Mohammad
authors.forename: Vicky W W
authors.initials: VW
authors.lastname: Tsai
authors.forename: Marc J
authors.initials: MJ
authors.lastname: Ruitenberg
authors.forename: Masoud
authors.initials: M
authors.lastname: Hassanpour
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Prue H
authors.initials: PH
authors.lastname: Hart
authors.forename: Samuel N
authors.initials: SN
authors.lastname: Breit
authors.forename: Paul E
authors.initials: PE
authors.lastname: Sawchenko
authors.forename: David A
authors.initials: DA
authors.lastname: Brown
mesh_terms: D000818:Animals; D018845:CD11 Antigens / Q000378:metabolism; D002465:Cell Movement; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000502:physiology*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000139:chemically induced / Q000276:immunology / Q000473:pathology; D005260:Female; D000068876:Fingolimod Hydrochloride; D007108:Immune Tolerance; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009333:Neck; D011409:Propylene Glycols; D016548:Prosencephalon / Q000276:immunology* / Q000473:pathology; D013110:Sphingosine / Q000031:analogs & derivatives; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
chemical_list: D018845:CD11 Antigens; D011409:Propylene Glycols; D000068876:Fingolimod Hydrochloride; D013110:Sphingosine