title: Stem cell-loaded nanofibrous patch promotes the regeneration of infarcted myocardium with functional improvement in rat model.
abstract: Myocardial infarction (MI) leads to the loss of cardiomyocytes, followed by left ventricular (LV) remodeling and cardiac dysfunction. The authors hypothesize that an elastic, biodegradable nanofibrous cardiac patch loaded with mesenchymal stem cells (MSC) could restrain LV remodeling and improve cardiac function after MI. Poly(ε-caprolactone)/gelatin (PG) nanofibers were fabricated by electrospinning, and the nanofibers displayed a porous and uniform nanofibrous structure with a diameter of 244±51nm. An MI model was established by ligation of the left anterior descending coronary artery of female Sprague-Dawley rats. The PG nanofibrous patch seeded with MSC, isolated from rat bone marrow, was implanted on the epicardium of the infarcted region of the LV wall of the heart. After transplantation, the PG-cell patch restricted the expansion of the LV wall effectively and reduced the scar size, and the density of the microvessels increased. Cells within the patch were able to migrate towards the scar tissue, and promoted new blood vessel formation at the infarct site. Angiogenesis and the cardiac functions improved significantly after 4weeks of implantation. The MSC-seeded PG nanofibrous patches are demonstrated to provide sufficient mechanical support, to induce angiogenesis and to accelerate cardiac repair in a rat model of MI. The study highlights the positive impact of implantation of an MSC-seeded PG nanofibrous patch as a novel constituent for MI repair.
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, China; NUS Graduate School for Integrative Sciences & Engineering, National University of Singapore, Singapore.
authors.forename: Dan
authors.initials: D
authors.lastname: Kai
authors.affiliation: Department of Anatomy, Histology and Embryology, Shanghai Medical School of Fudan University, 138 Yixueyuan Rd, Shanghai 200032, China.
authors.forename: Qiang-Li
authors.initials: QL
authors.lastname: Wang
authors.affiliation: Department of Anatomy, Histology and Embryology, Shanghai Medical School of Fudan University, 138 Yixueyuan Rd, Shanghai 200032, China.
authors.forename: Hai-Jie
authors.initials: HJ
authors.lastname: Wang
authors.affiliation: Center for Nanofibers and Nanotechnology, Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, 2 Engineering Drive 3, Singapore.
authors.forename: Molamma P
authors.initials: MP
authors.lastname: Prabhakaran
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, China.
authors.forename: Yanzhong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Anatomy, Histology and Embryology, Shanghai Medical School of Fudan University, 138 Yixueyuan Rd, Shanghai 200032, China. Electronic address: yztan@shmu.edu.cn.
authors.forename: Yu-Zhen
authors.initials: YZ
authors.lastname: Tan
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, China; NUS Graduate School for Integrative Sciences & Engineering, National University of Singapore, Singapore; Center for Nanofibers and Nanotechnology, Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, 2 Engineering Drive 3, Singapore; Department of Mechanical Engineering, National University of Singapore, 9 Engineering Drive 1, Singapore. Electronic address: seeram@nus.edu.sg.
authors.forename: Seeram
authors.initials: S
authors.lastname: Ramakrishna
mesh_terms: D000818:Animals; D006321:Heart / Q000502:physiology*; D008297:Male; D009203:Myocardial Infarction / Q000503:physiopathology*; D057139:Nanofibers; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D013234:Stem Cells / Q000166:cytology*
keywords: Cardiac patch; Electrospun nanofibers; Improvement of cardiac function; Mesenchymal stem cells; Myocardial infarction