title: Alpha 2 integrin gene (ITGA2) polymorphism in renal transplant recipients with and without drug induced gingival overgrowth.
abstract: BACKGROUND
  Variances in fibroblasts' α2β1 integrin intensity may lead to altered adhesion to type I collagen and consequently to suppression of phagocytosis which may be one of the mechanisms for drug induced gingival overgrowth. The present study aimed to evaluate the genotype and allele frequencies of α2 integrin +807 gene in renal transplant patients with and without gingival overgrowth.
  
  
  METHODS
  Seventy renal transplant patients with cyclosporine A (CsA)-induced gingival overgrowth (CsA GO+) were enrolled. Renal transplant patients without GO medicated with CsA (CsA GO-; n=79) and tacrolimus (Tac; n=52) served as controls. DNA was obtained from peripheral blood and ITGA2 +807C/T polymorphism was genotyped by polymerase chain reaction and restriction fragment length polymorphism method. Clinical parameters including probing depth and plaque, papilla bleeding and hyperplasia indexes were recorded. Chi-square, Kruskal-Wallis and Mann-Whitney tests were used in statistical analysis.
  
  
  RESULTS
  Clinical parameters of CsA GO+ group were significantly higher than those of the CsA GO- and Tac groups (p<0.05). ITGA2 807C/T genotype and allele frequencies of study groups were similar (p>0.05).
  
  
  CONCLUSIONS
  Within the limits of the present study it can be concluded that ITGA2 +807 gene polymorphism is not associated with susceptibility to CsA-induced GO.
authors.affiliation: Ege University, School of Dentistry, Department of Periodontology, Izmir, Turkey. Electronic address: ali.gurkan@ege.edu.tr.
authors.forename: Ali
authors.initials: A
authors.lastname: Gürkan
authors.affiliation: Ege University, School of Dentistry, Department of Periodontology, Izmir, Turkey.
authors.forename: Gülnur
authors.initials: G
authors.lastname: Emingil
authors.affiliation: Ege University, School of Dentistry, Department of Periodontology, Izmir, Turkey.
authors.forename: Beral
authors.initials: B
authors.lastname: Afacan
authors.affiliation: Ege University, School of Medicine, Department of Pediatrics, Molecular Medicine Laboratory Izmir, Turkey.
authors.forename: Afig
authors.initials: A
authors.lastname: Berdeli
authors.affiliation: Ege University, School of Dentistry, Department of Periodontology, Izmir, Turkey.
authors.forename: Gül
authors.initials: G
authors.lastname: Atilla
mesh_terms: D000328:Adult; D016572:Cyclosporine / Q000009:adverse effects*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D019214:Gingival Overgrowth / Q000139:chemically induced*; D006801:Humans; D007166:Immunosuppressive Agents / Q000009:adverse effects*; D039421:Integrin alpha2 / Q000235:genetics*; D016030:Kidney Transplantation; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D016559:Tacrolimus / Q000009:adverse effects*
chemical_list: C056564:ITGA2B protein, human; D007166:Immunosuppressive Agents; D039421:Integrin alpha2; D016572:Cyclosporine; D016559:Tacrolimus
keywords: Cyclosporine; Gingiva; Integrin; Overgrowth; Polymorphism