title: Inhibition of hepatitis B virus cccDNA by siRNA in transgenic mice.
abstract: The elimination of viral covalently closed circular DNA (cccDNA) from the nucleus of infected hepatocytes is an obstacle to achieving sustained viral clearance during antiviral therapy of chronic hepatitis B virus (HBV) infection. The aim of our study was to determine whether treatment with siRNA is able to suppress viral cccDNA amplification using a HBV-transgenic mice model. The experimental results revealed that siRNAs can serve as efficient alternative anti-HBV agents, because they showed better inhibitory effect on viral replication and antigen expression in transgenic mice. More importantly, the siRNA markedly inhibited HBV cccDNA amplification.
authors.affiliation: Department of Clinical Laboratory, The Affiliated First Hospital of Harbin Medical University, Harbin, 150001, China.
authors.forename: Guiqiu
authors.initials: G
authors.lastname: Li
authors.forename: Guotao
authors.initials: G
authors.lastname: Jiang
authors.forename: Juan
authors.initials: J
authors.lastname: Lu
authors.forename: Shulan
authors.initials: S
authors.lastname: Chen
authors.forename: Lanying
authors.initials: L
authors.lastname: Cui
authors.forename: Jundong
authors.initials: J
authors.lastname: Jiao
authors.forename: Yongchen
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D004270:DNA, Circular / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D004279:DNA, Viral / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D005786:Gene Expression Regulation; D006514:Hepatitis B Surface Antigens / Q000378:metabolism; D006513:Hepatitis B e Antigens / Q000378:metabolism; D006515:Hepatitis B virus / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics*; D014779:Virus Replication / Q000235:genetics
chemical_list: D004270:DNA, Circular; D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D012333:RNA, Messenger; D034741:RNA, Small Interfering