title: Isolation and identification of acaricidal compounds in Eupatorium adenophorum petroleum ether extract and determination of their acaricidal activity against Psoroptes cuniculi.
abstract: We used multiple silica gel column chromatography and thin-layer chromatography coupled with (1)H nuclear magnetic resonance (NMR) and (13)C NMR to separate and identify the active acaricidal ingredients in Eupatorium adenophorum petroleum ether extract. The acaricidal activity of each compound was tested against Psoroptes cuniculi in vitro. Three compounds had strong acaricidal activity against P. cuniculi in vitro. The insecticidal effect of 0.5% compound 9β-hydroxy-ageraphorone was better than the insecticidal effect of fenvalerate, and compounds 9-oxo-ageraphorone and 9-oxo-10,11-dehydro-ageraphorone exhibited higher insecticidal effects than 9β-hydroxy-ageraphorone. Thus, the E. adenophorum petroleum ether extract contains an effective composition of acaricides that could potentially be developed as a promising plant-origin acaricide.
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; College of Life Sciences, Leshan Normal University, Le'shan 614004, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Nong
authors.affiliation: College of Life Sciences, Leshan Normal University, Le'shan 614004, China.
authors.forename: Shu-Hua
authors.initials: SH
authors.lastname: Li
authors.affiliation: College of Life Sciences, Leshan Normal University, Le'shan 614004, China.
authors.forename: Feng-Zheng
authors.initials: FZ
authors.lastname: Chen
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Jia-Hai
authors.initials: JH
authors.lastname: Wang
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Sichuan Engineering Research Center for New Veterinary Drug, Cheng'du 611130, China.
authors.forename: Chun-Lin
authors.initials: CL
authors.lastname: Fang
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Tian-Fei
authors.initials: TF
authors.lastname: Liu
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Ran
authors.initials: R
authors.lastname: He
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Gu
authors.affiliation: College of Life and Basic Sciences, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Xue-Rong
authors.initials: XR
authors.lastname: Peng
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China. Electronic address: guangyouyang@hotmail.com.
authors.forename: Guang-You
authors.initials: GY
authors.lastname: Yang
mesh_terms: D056810:Acaricides / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity; D036603:Ageratina / Q000737:chemistry*; D000818:Animals; D004986:Ether / Q000737:chemistry*; D005260:Female; D007928:Lethal Dose 50; D008297:Male; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity; D040042:Psoroptidae / Q000187:drug effects*
chemical_list: D056810:Acaricides; D010936:Plant Extracts; D004986:Ether
keywords: (1)H NMR and (13)C NMR; Acaricidal activity; Eupatorium adenophorum; Multiple silica gel column chromatography