title: Polish natural bee honeys are anti-proliferative and anti-metastatic agents in human glioblastoma multiforme U87MG cell line.
abstract: Honey has been used as food and a traditional medicament since ancient times. However, recently many scientists have been concentrating on the anti-oxidant, anti-proliferative, anti-inflammatory and other properties of honey. In this study, we investigated for the first time an anticancer effect of different honeys from Poland on tumor cell line - glioblastoma multiforme U87MG. Anti-proliferative activity of honeys and its interferences with temozolomide were determined by a cytotoxicity test and DNA binding by [H3]-thymidine incorporation. A gelatin zymography was used to conduct an evaluation of metalloproteinases (MMP-2 and MMP-9) expression in U87MG treatment with honey samples. The honeys were previously tested qualitatively (diastase activity, total phenolic content, lead and cadmium content). The data demonstrated that the examined honeys have a potent anti-proliferative effect on U87MG cell line in a time- and dose-dependent manner, being effective at concentrations as low as 0.5% (multifloral light honey - viability 53% after 72 h of incubation). We observed that after 48 h, combining honey with temozolomide showed a significantly higher inhibitory effect than the samples of honey alone. We observed a strong inhibition of MMP-2 and MMP-9 for the tested honeys (from 20 to 56% and from 5 to 58% compared to control, respectively). Our results suggest that Polish honeys have an anti-proliferative and anti-metastatic effect on U87MG cell line. Therefore, natural bee honey can be considered as a promising adjuvant treatment for brain tumors.
authors.affiliation: Department of Bromatology, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Moskwa
authors.affiliation: Department of Bromatology, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Maria H
authors.initials: MH
authors.lastname: Borawska
authors.affiliation: Department of Bromatology, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Markiewicz-Zukowska
authors.affiliation: Department of Bromatology, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Puscion-Jakubik
authors.affiliation: Department of Bromatology, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Sylwia K
authors.initials: SK
authors.lastname: Naliwajko
authors.affiliation: Department of Bromatology, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Socha
authors.affiliation: Department of Bromatology, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Soroczynska
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000032:analysis / Q000494:pharmacology*; D001516:Bees; D001688:Biological Products / Q000032:analysis / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003606:Dacarbazine / Q000031:analogs & derivatives / Q000494:pharmacology; D005909:Glioblastoma / Q000188:drug therapy*; D006722:Honey / Q000032:analysis; D006801:Humans; D009362:Neoplasm Metastasis / Q000517:prevention & control; D000077204:Temozolomide
chemical_list: D000970:Antineoplastic Agents; D001688:Biological Products; D003606:Dacarbazine; D000077204:Temozolomide