title: Effect of dose and route of immunisation on the immune response induced in cattle by heterologous Bacille Calmette-Guerin priming and recombinant adenoviral vector boosting.
abstract: BCG is used experimentally as a vaccine against tuberculosis (TB), induced by Mycobacterium bovis, in cattle (bTB). However, the efficacy of BCG is variable in humans, cattle and guinea pigs. An adenoviral vector expressing Antigen 85A (Ad5Ag85A) has enhanced protection against TB in mice when used in combination with BCG for prime-boost experiments. However, the route of immunisation affects the degree of protection seen. This work examines the immunogenicity of a new vectored vaccine (Ad5-TBF) that expresses Ag85A, Rv0287, Rv0288 and Rv0251c to explore the effects of dose of adenoviral boost and route of inoculation on immunogenicity. We found that 2×10(9) infectious units (iu) delivered intradermally conferred the most consistent and strongest responses of the different regimes tested.
authors.affiliation: Bovine TB, AHVLA-Weybridge, New Haw, Addlestone, Surrey KT15 3NB, UK.
authors.forename: G
authors.initials: G
authors.lastname: Dean
authors.affiliation: Bovine TB, AHVLA-Weybridge, New Haw, Addlestone, Surrey KT15 3NB, UK.
authors.forename: D
authors.initials: D
authors.lastname: Clifford
authors.affiliation: The Jenner Institute Old Road Campus Research Building Oxford University, Roosevelt Drive, Oxford OX3 7DQ, UK.
authors.forename: S
authors.initials: S
authors.lastname: Gilbert
authors.affiliation: The Jenner Institute Old Road Campus Research Building Oxford University, Roosevelt Drive, Oxford OX3 7DQ, UK.
authors.forename: H
authors.initials: H
authors.lastname: McShane
authors.affiliation: Bovine TB, AHVLA-Weybridge, New Haw, Addlestone, Surrey KT15 3NB, UK.
authors.forename: R G
authors.initials: RG
authors.lastname: Hewinson
authors.affiliation: Bovine TB, AHVLA-Weybridge, New Haw, Addlestone, Surrey KT15 3NB, UK.
authors.forename: H M
authors.initials: HM
authors.lastname: Vordermeier
authors.affiliation: Bovine TB, AHVLA-Weybridge, New Haw, Addlestone, Surrey KT15 3NB, UK. Electronic address: Bernardo.Villarreal@ahvla.gsi.gov.uk.
authors.forename: B
authors.initials: B
authors.lastname: Villarreal-Ramos
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000235:genetics; D001500:BCG Vaccine / Q000008:administration & dosage*; D002417:Cattle; D004306:Dose-Response Relationship, Immunologic; D005822:Genetic Vectors; D007115:Immunization Schedule; D007117:Immunization, Secondary / Q000662:veterinary; D007271:Injections, Intradermal; D008297:Male; D009163:Mycobacterium bovis / Q000235:genetics* / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014380:Tuberculosis, Bovine / Q000276:immunology / Q000517:prevention & control*
chemical_list: D000942:Antigens, Bacterial; D001500:BCG Vaccine; D011993:Recombinant Fusion Proteins; C065036:antigen 85, Mycobacterium bovis
keywords: Adenovirus; Mycobacteria; Route of immunisation; Tuberculosis; Vaccine