title: Measurement of intracellular Ca2+ release in intact and permeabilized cells using 45Ca2+.
abstract: Ca(2+) is an important ion that controls almost every function in a cell. Activator Ca(2+) can be released from intracellular Ca(2+) stores, and there are various ways to study this release. Here, we introduce a technique that uses radioactive (45)Ca(2+) to quantitatively measure the unidirectional release of Ca(2+) from the nonmitochondrial Ca(2+) stores in monolayers of cultured cells. This technique can be used in cells with an intact plasma membrane as well as in cells in which the plasma membrane has been permeabilized.
authors.affiliation: Laboratory of Molecular and Cellular Signaling, Department of Cellular and Molecular Medicine, KU Leuven Campus Gasthuisberg O&N I, 3000 Leuven, Belgium.
authors.forename: Ludwig
authors.initials: L
authors.lastname: Missiaen
authors.forename: Tomas
authors.initials: T
authors.lastname: Luyten
authors.forename: Geert
authors.initials: G
authors.lastname: Bultynck
authors.forename: Jan B
authors.initials: JB
authors.lastname: Parys
authors.forename: Humbert
authors.initials: H
authors.lastname: De Smedt
mesh_terms: D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes / Q000378:metabolism*; D002463:Cell Membrane Permeability; D006801:Humans; D017136:Ion Transport
chemical_list: D002132:Calcium Radioisotopes; D002118:Calcium