title: Galactosylated electrospun membranes for hepatocyte sandwich culture.
abstract: In this work, we developed a galactocylated electrospun polyurethane membrane for sandwich culture of hepatocyte sandwich culture. The electrospun fibrous membranes were bio-functionalized with galactose molecules by a UV-crosslinked layer-by-layer polyelectrolyte multilayer deposition technique. The galactosylated electrospun membranes were employed as a top support membrane for the sandwich culture of HepG2/C3A cells on a collagen substrate. Our results demonstrate that HepG2/C3A cells covered by the galactosylated PU membranes form multi-cellular aggregates and lead to improved albumin secretion ability compared to the control membranes (unmodified PU or poly(ethylene imine)-modified PU). Our study reveals the potential of galactosylated electrospun membranes in the application of liver tissue engineering and the regeneration of liver-tissue substitutes.
authors.affiliation: Department of Chemical Engineering, National Taiwan University, No. 1, Roosevelt Rd., Sec. 4, Taipei 106, Taiwan.
authors.forename: Hsiu-Wen
authors.initials: HW
authors.lastname: Chien
authors.affiliation: R&D Center for Membrane Technology and Department of Chemical Engineering, Chung Yuan Christian University, Chungli, Taoyuan, Taiwan.
authors.forename: Juin-Yih
authors.initials: JY
authors.lastname: Lai
authors.affiliation: Department of Chemical Engineering, National Taiwan University, No. 1, Roosevelt Rd., Sec. 4, Taipei 106, Taiwan. Electronic address: weibortsai@ntu.edu.tw.
authors.forename: Wei-Bor
authors.initials: WB
authors.lastname: Tsai
mesh_terms: D000327:Adsorption; D018929:Cell Culture Techniques; D004573:Electrolytes / Q000737:chemistry; D005690:Galactose / Q000737:chemistry*; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000166:cytology*; D006801:Humans; D011140:Polyurethanes / Q000138:chemical synthesis / Q000737:chemistry*; D013499:Surface Properties; D014407:Tumor Cells, Cultured
chemical_list: D004573:Electrolytes; D011140:Polyurethanes; D005690:Galactose
keywords: Electrospun membrane; Hepatocytes; Polyelectrolyte multilayer deposition; Sandwich culture