title: Polymorphism of costimulatory molecules (CTLA4, ICOS, PD.1 and CD28) and allogeneic hematopoietic stem cell transplantation in Iranian patients.
abstract: Costimulatory molecules are important factors determining the outcome of bone marrow transplant. Because the host ability in costimulatory molecule function may be affected by gene polymorphisms, the aim of the present study was to investigate the effect of CTLA4, ICOS, PD.1 and CD28 gene polymorphisms in outcome of bone marrow transplant patients. A total of 72 recipients were included in this study. CTLA4 (-1722, -1661, -318, +49), ICOS (+1720), CD28 (+17) and PD.1 (PD.1.3, PD.1.9) gene polymorphisms were evaluated by PCR-RFLP. The results showed that no differences in the distribution of all mentioned costimulatory molecules genotypes and alleles were observed in the Graft Versus Host Disease (GVHD) group compared to the non-GVHD group. After gender classification, there is a significant association between GA genotype (CTLA4-1661) in male group with GVHD than without GVHD (p=0.03). Also, in this study we found significant associations between CC genotype and C allele of PD.1.9, and TT genotype and T allele of CD28 that had more frequency in grades 2-4 (p=0.04. p=0.02, p=0.01, p=0.003, respectively). Results indicate that the CC genotype and C allele of PD.1.9 and TT genotype and the T allele of CD28 are genetic risk factors for development of a severe grade of GVHD. This subject needs to be studied in different population.
authors.affiliation: Transplant Research Center, Shiraz University of Medical Sciences , Shiraz , Iran .
authors.forename: Mahdiyar
authors.initials: M
authors.lastname: Iravani-Saadi
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Karimi
authors.forename: Ramin
authors.initials: R
authors.lastname: Yaghobi
authors.forename: Bita
authors.initials: B
authors.lastname: Geramizadeh
authors.forename: Mani
authors.initials: M
authors.lastname: Ramzi
authors.forename: Ahmad
authors.initials: A
authors.lastname: Niknam
authors.forename: Arefeh
authors.initials: A
authors.lastname: Pourfathollah
mesh_terms: D000293:Adolescent; D000328:Adult; D000483:Alleles; D018106:CD28 Antigens / Q000235:genetics*; D060908:CTLA-4 Antigen / Q000235:genetics*; D002648:Child; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006086:Graft vs Host Disease / Q000209:etiology*; D006239:Haplotypes; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects; D006801:Humans; D060889:Inducible T-Cell Co-Stimulator Protein / Q000235:genetics*; D007492:Iran; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D061026:Programmed Cell Death 1 Receptor / Q000235:genetics*; D014184:Transplantation, Homologous; D055815:Young Adult
chemical_list: D018106:CD28 Antigens; D060908:CTLA-4 Antigen; D060889:Inducible T-Cell Co-Stimulator Protein; D061026:Programmed Cell Death 1 Receptor