title: Comparison of sesion severity, distribution, and colonic mucin expression in pigs with acute swine dysentery following oral inoculation with "Brachyspira hampsonii" or Brachyspira hyodysenteriae.
abstract: Swine dysentery is classically associated with infection by Brachyspira hyodysenteriae, the only current officially recognized Brachyspira sp. that consistently imparts strong beta-hemolysis on blood agar. Recently, several strongly beta-hemolytic Brachyspira have been isolated from swine with clinical dysentery that are not identified as B. hyodysenteriae by PCR including the recently proposed species "Brachyspira hampsonii." In this study, 6-week-old pigs were inoculated with either a clinical isolate of "B. hampsonii" (EB107; n = 10) clade II or a classic strain of B. hyodysenteriae (B204; n = 10) to compare gross and microscopic lesions and alterations in colonic mucin expression in pigs with clinical disease versus controls (n = 6). Gross lesions were similar between infected groups. No histologic difference was observed between infected groups with regard to neutrophilic inflammation, colonic crypt depth, mucosal ulceration, or hemorrhage. Histochemical and immunohistochemical evaluation of the apex of the spiral colon revealed decreased expression of sulphated mucins, decreased expression of MUC4, and increased expression of MUC5AC in diseased pigs compared to controls. No difference was observed between diseased pigs in inoculated groups. This study reveals significant alterations in colonic mucin expression in pigs with acute swine dysentery and further reveals that these and other microscopic changes are similar following infection with "B. hampsonii" clade II or B. hyodysenteriae.
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA.
authors.forename: B L
authors.initials: BL
authors.lastname: Wilberts
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA.
authors.forename: P H
authors.initials: PH
authors.lastname: Arruda
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA.
authors.forename: J M
authors.initials: JM
authors.lastname: Kinyon
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA.
authors.forename: D M
authors.initials: DM
authors.lastname: Madson
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA.
authors.forename: T S
authors.initials: TS
authors.lastname: Frana
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA burrough@iastate.edu.
authors.forename: E R
authors.initials: ER
authors.lastname: Burrough
mesh_terms: D000818:Animals; D057138:Bacterial Shedding; D017400:Brachyspira / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D017401:Brachyspira hyodysenteriae / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity; D003106:Colon / Q000473:pathology; D004403:Dysentery / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D005243:Feces / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D007150:Immunohistochemistry / Q000662:veterinary; D017404:In Situ Hybridization, Fluorescence / Q000662:veterinary; D009077:Mucins / Q000378:metabolism; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology / Q000473:pathology*
chemical_list: D009077:Mucins
keywords: brachyspira; histochemistry; mucin; swine dysentery; “Brachyspira hampsonii; ” Brachyspira hyodysenteriae