title: Effects of plant biomass on denitrifying genes in subsurface-flow constructed wetlands.
abstract: The effect of Typha latifolia and its litter on density and abundance of three denitrifying genes (nirS, nirK and nosZ) were investigated in six laboratory-scale SSF CW microcosms. Results showed that the copy numbers of nirS, nirK and nosZ in wetland microcosms were ranged between 10(8)-10(9), 10(6)-10(7) and 10(7)-10(8) copies g(-1), respectively. The presence of T. latifolia encouraged the growth of nirK containing bacteria. Addition of cattail litter could greatly stimulate the growth of bacteria containing nirS and nosZ gene. Path analysis illustrated that the presence of plants and litters had no significant direct impact on denitrifying genes, while it affected the denitrifying genes via alteration of dissolved oxygen and carbon sources.
authors.affiliation: Key Laboratory of Yangtze Water Environment of Ministry of the State Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, PR China; Department of Applied Ecology, Faculty of Environmental Sciences, Czech University of Life Sciences Prague, 16521 Prague 6, Czech Republic.
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Key Laboratory of Yangtze Water Environment of Ministry of the State Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wen
authors.affiliation: Key Laboratory of Yangtze Water Environment of Ministry of the State Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Department of Applied Ecology, Faculty of Environmental Sciences, Czech University of Life Sciences Prague, 16521 Prague 6, Czech Republic. Electronic address: vymazal@yahoo.com.
authors.forename: Jan
authors.initials: J
authors.lastname: Vymazal
mesh_terms: D001419:Bacteria / Q000201:enzymology* / Q000235:genetics; D018533:Biomass; D058440:Denitrification / Q000235:genetics*; D005798:Genes, Bacterial; D008954:Models, Biological; D050901:Nitrate Reductase / Q000235:genetics / Q000378:metabolism; D010944:Plants / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D053833:Wetlands
chemical_list: D012336:RNA, Ribosomal, 16S; D050901:Nitrate Reductase
keywords: Cattail litter; Denitrifying genes; Nitrate removal; Subsurface flow constructed wetlands (SSF CWs); Typha latifolia