title: Photodynamic inactivation of enveloped virus in protein plasma preparations by solid-phase fullerene-based photosensitizer.
abstract: BACKGROUND
  The problem of transfusion-transmitted infections still remains serious and actual for health care despite the detailed testing of donors. Human immunodeficiency virus, hepatitis B and C viruses and human cytomegalovirus are among the most dangerous pathogens that can be transmitted with blood. Previously, a composition consisting of fullerene layer applied on silica gel particles was shown to inactivate influenza virus up to complete loss of infectivity.
  
  
  METHODS
  In the present study the unit has been developed with source of irradiation whose spectrum is appropriate for solid-phase fullerene. The ability of the unit to inactivate the enveloped influenza virus in protein fraction of donor blood has been studied.
  
  
  RESULTS
  It was shown that at optimized conditions complete inactivation of enveloped virus of extremely high initial titer (7.0-9.5 log 10 EID 50/0.2 mL) in the solution of albumin was achieved after as short time as 30 min of irradiation. This process did not affect the oxidative metabolism of neutrophils and membranes of erythrocytes evaluated by NBT reduction test and morphological analysis of erythrocytes, respectively.
  
  
  CONCLUSIONS
  The data obtained suggests that the method described can be recommended for further development and optimization of the procedure of inactivation of viruses in the preparations of the plasma of donor blood.
authors.affiliation: State Optical Institute, St. Petersburg, Russia.
authors.forename: I M
authors.initials: IM
authors.lastname: Belousova
authors.affiliation: State Optical Institute, St. Petersburg, Russia.
authors.forename: I M
authors.initials: IM
authors.lastname: Kislyakov
authors.affiliation: State Optical Institute, St. Petersburg, Russia.
authors.forename: T D
authors.initials: TD
authors.lastname: Muraviova
authors.affiliation: State Optical Institute, St. Petersburg, Russia.
authors.forename: A M
authors.initials: AM
authors.lastname: Starodubtsev
authors.affiliation: State Optical Institute, St. Petersburg, Russia.
authors.forename: T K
authors.initials: TK
authors.lastname: Kris'ko
authors.affiliation: Institute of Hematology and Transfusiology, St. Petersburg, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Selivanov
authors.affiliation: Institute of Hematology and Transfusiology, St. Petersburg, Russia.
authors.forename: N P
authors.initials: NP
authors.lastname: Sivakova
authors.affiliation: Institute of Hematology and Transfusiology, St. Petersburg, Russia.
authors.forename: I S
authors.initials: IS
authors.lastname: Golovanova
authors.affiliation: Institute of Hematology and Transfusiology, St. Petersburg, Russia.
authors.forename: S D
authors.initials: SD
authors.lastname: Volkova
authors.affiliation: Influenza Research Institute, St. Petersburg, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Shtro
authors.affiliation: Influenza Research Institute, St. Petersburg, Russia. Electronic address: zarubaev@influenza.spb.ru.
authors.forename: V V
authors.initials: VV
authors.lastname: Zarubaev
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000187:drug effects* / Q000528:radiation effects*; D004285:Dogs; D037741:Fullerenes / Q000008:administration & dosage* / Q000737:chemistry; D009980:Influenza A virus / Q000187:drug effects* / Q000502:physiology* / Q000528:radiation effects; D061985:Madin Darby Canine Kidney Cells; D044367:Phase Transition; D010778:Photochemotherapy / Q000379:methods; D038301:Virus Inactivation / Q000187:drug effects* / Q000528:radiation effects*
chemical_list: D001798:Blood Proteins; D037741:Fullerenes; C069837:fullerene C60
keywords: Blood products; Enveloped virus; Fullerene C60; Photoinactivation