title: Antioxidant activity and preventive effect of aqueous leaf extract of Aloe Vera on gentamicin-induced nephrotoxicity in male Wistar rats.
abstract: OBJECTIVE
  In the present study, the nephroprotective and curative effects of aqueous extract of AV on gentamicin-induced tubular toxicity in male Wistar rats were investigated. Antioxidant activity, phenolic and flavonoid contents of AV were also determined.
  
  
  METHODS
  In a preclinical study, 60 male Wistar rats were designated into 6 groups of 10 and treated as follows: Group I: Treated with vehicle (distilled water) and kept as control. Group II: Received 300 mg/kg/d AV for three days then, AV plus GM (80 mg/kg; intraperitoneally) for 7 more days and sacrificed. Group III: Received 300 mg/kg AV orally for 10 days, then sacrificed. Group IV: Received GM for 7 days and sacrificed. Group V: Received GM for 7 days and saline for ten days and sacrificed on 17th day. Group VI: Received GM for 7 days, then AV for 10 days and sacrificed.
  
  
  RESULTS
  The levels of flavonoid and phenolic compounds in Aloe vera extract were 43 ± 3.8 mg/g (Rutin equivalent) and 80.1 ± 3.8 mg/g (Gallic acid equivalent), respectively. The antioxidant activity of the extract was 38%. The intensity of nephrotoxicity in group 2 that received AV 300 mg/kg/d as prophylaxis was not significantly different from the group I and from the group II which treated with 300 mg/kg/d AV without GM. This finding reveals that AV as prophylaxis could potentially attenuate the GM-induced nephrotoxicity.
  
  
  CONCLUSIONS
  Aole vera significantly protects the renal cells and reduces the severity of tubular damage caused by gentamicin. However, it cannot regenerate tubular damage.
authors.affiliation: Departments of Pathology Isfahan University of Medical Sciences, Isfahan, Iran;
authors.forename: A
authors.initials: A
authors.lastname: Baradaran
authors.affiliation: Departments of Nephrology, Division of Nephropathology, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Nasri
authors.affiliation: Departments of Water and Electrolytes Research Center, Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Nematbakhsh
authors.affiliation: Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Rafieian-Kopaei
mesh_terms: D000504:Aloe / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D005839:Gentamicins / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D008297:Male; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D005839:Gentamicins; D010936:Plant Extracts