title: Tissue engineering scaffolds of mesoporous magnesium silicate and poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) composite.
abstract: Mesoporous magnesium silicate (m-MS) and poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCL-PEG-PCL) composite scaffolds were fabricated by solvent-casting and particulate leaching method. The results suggested that the incorporation of m-MS into PCL-PEG-PCL could significantly improve the water adsorption of the m-MS/PCL-PEG-PCL composite (m-MPC) scaffolds. The in vitro degradation behavior of m-MPC scaffolds were determined by testing weight loss of the scaffolds after soaking into phosphate buffered saline (PBS), and the result showed that the degradation of m-MPC scaffolds was obviously enhanced by addition of m-MS into PCL-PEG-PCL after soaking for 10 weeks. Proliferation of MG63 cells on m-MPC was significantly higher than MPC scaffolds at 4 and 7 days. ALP activity on the m-MPC was obviously higher than MPC scaffolds at 7 days, revealing that m-MPC could promote cell differentiation. Histological evaluation showed that the introduction of m-MS into PCL-PEG-PCL enhanced the efficiency of new bone formation when the m-MPC scaffolds implanted into bone defect of rabbits. The results suggested that the inorganic/organic composite of m-MS and PCL-PEG-PCL scaffolds exhibited good biocompatibility, degradability and osteogenesis.
authors.affiliation: Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai, 200433, People's Republic of China.
authors.forename: Dawei
authors.initials: D
authors.lastname: He
authors.forename: Wei
authors.initials: W
authors.lastname: Dong
authors.forename: Songchao
authors.initials: S
authors.lastname: Tang
authors.forename: Jie
authors.initials: J
authors.lastname: Wei
authors.forename: Zhenghui
authors.initials: Z
authors.lastname: Liu
authors.forename: Xiaojiang
authors.initials: X
authors.lastname: Gu
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Han
authors.initials: H
authors.lastname: Guo
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Niu
mesh_terms: D020341:Absorbable Implants; D000042:Absorption; D000818:Animals; D018786:Bone Substitutes / Q000138:chemical synthesis*; D002460:Cell Line; D049109:Cell Proliferation; D003460:Crystallization / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005264:Femoral Fractures / Q000473:pathology / Q000601:surgery*; D006801:Humans; D017633:Magnesium Silicates / Q000737:chemistry*; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D010012:Osteogenesis; D011091:Polyesters / Q000138:chemical synthesis*; D011092:Polyethylene Glycols / Q000138:chemical synthesis*; D016062:Porosity; D011817:Rabbits; D013499:Surface Properties; D023822:Tissue Engineering / Q000295:instrumentation*; D054457:Tissue Scaffolds; D016896:Treatment Outcome; D014867:Water
chemical_list: D018786:Bone Substitutes; D017633:Magnesium Silicates; D011091:Polyesters; C515340:poly(epsilon-caprolactone)-b-poly(ethyleneglycol)-b-poly(epsilon-caprolactone); D014867:Water; C005013:Florisil; D011092:Polyethylene Glycols