title: Comparison of intravesical application of chondroitin sulphate and colchicine in rat protamine/lipopolysaccharide induced cystitis model.
abstract: OBJECTIVE
  To investigate beneficial effect of the readily available colchicine through its intravesical application on protamine/lipopolysaccharide induced interstitial cystitis model in rat and to compare its efficacy to the chondroitin sulphate available for clinical use.
  
  
  METHODS
  Twenty-four Wistar female rats were assigned to control (C), interstitial cystitis (IC), chondroitin sulphate (CS) and colchicine (Col) groups. IC, CS and Col groups received protamine sulphate and lipopolysaccharide (PS/LPS) instillation. Testing agents CS and Col were administered a day after PS/LPS inoculation into the bladders. Rats in Group C received saline solution. CS and Col groups received 1 mL CS (0.2%) and 1 mL Col (0.05 mg/mL). The treatment agents were left in bladders for one hour's duration. Animals were sacrificed 5 days after the inoculation and the bladder tissues were examined histologically to evaluate the amount of extravasated leucocytes, mast cell concentration (by counting total number of cells per 10 high power field (hpf; 1 hpf = ×400 magnification) as well as interstitial tissue edema for each bladder.
  
  
  RESULTS
  Intravesical application of CS reduced significantly the leucocyte and mast cell infiltration as well as interstitial edema compared to group C . The level of reduction in leucocyte and mast cell infiltration in Col group was comparable to that of CS, although the interstitial edema was not resolved.
  
  
  CONCLUSIONS
  Intravesical administration of Col decreased leucocyte and mast cell infiltration to the same extent of CS in PS/LPS induced bladder inflammation in rat. Col may be an alternative to other treatment modalities for painful bladder conditions such as IC.
authors.affiliation: Department of Urology, Maltepe University School of Medicine, Istanbul, Turkey. orhundr@hotmail.com.
authors.forename: Orhun
authors.initials: O
authors.lastname: Sinanoglu
authors.affiliation: Department of Pathology, Yeditepe University School of Medicine, Istanbul, Turkey.
authors.forename: Isin
authors.initials: I
authors.lastname: Dogan Ekici
authors.affiliation: Department of Urology, Maltepe University School of Medicine, Istanbul, Turkey.
authors.forename: Sinan
authors.initials: S
authors.lastname: Ekici
mesh_terms: D000283:Administration, Intravesical; D000818:Animals; D002809:Chondroitin Sulfates / Q000008:administration & dosage*; D003078:Colchicine / Q000008:administration & dosage*; D003556:Cystitis / Q000139:chemically induced / Q000188:drug therapy*; D018856:Cystitis, Interstitial; D004195:Disease Models, Animal; D005260:Female; D008070:Lipopolysaccharides; D011479:Protamines; D051381:Rats; D017208:Rats, Wistar
chemical_list: D008070:Lipopolysaccharides; D011479:Protamines; D002809:Chondroitin Sulfates; D003078:Colchicine