title: TEM analyses of chaetognath reproductive organs.
abstract: Transmission electron microscopy (TEM) enables analysis of subcellular organization. It is especially useful for describing the diverse array of cell types in the gonads and embryos of marine invertebrates. Here, I describe methods for preserving and embedding the reproductive organs of marine arrow worms for TEM, including procedures for staining thick sections for light microscopy and thin sections for TEM.
authors.affiliation: Biology Department, Truman State University, Kirksville, MO, USA.
authors.forename: George L
authors.initials: GL
authors.lastname: Shinn
mesh_terms: D000818:Animals; D005404:Fixatives / Q000737:chemistry; D005557:Formaldehyde / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry; D007448:Invertebrates / Q000033:anatomy & histology / Q000648:ultrastructure*; D046529:Microscopy, Electron, Transmission; D008867:Microtomy; D009865:Oocytes / Q000648:ultrastructure; D016613:Plastic Embedding; D011108:Polymers / Q000737:chemistry; D012098:Reproduction; D016707:Tissue Fixation
chemical_list: D005404:Fixatives; D011108:Polymers; D005557:Formaldehyde; D005976:Glutaral; C003043:paraform