title: Structure, properties and animal study of a calcium phosphate/calcium sulfate composite cement.
abstract: In-vitro and in-vivo studies have been conducted on an in-house-developed tetracalcium phosphate (TTCP)/dicalcium phosphate anhydrous (DCPA)/calcium sulfate hemihydrate (CSH)-derived composite cement. Unlike most commercial calcium-based cement pastes, the investigated cement paste can be directly injected into water and harden without dispersion. The viability value of cells incubated with a conditioned medium of cement extraction is >90% that of Al2O3 control and >80% that of blank medium. Histological examination reveals excellent bonding between host bone and cement without interposition of fibrous tissues. At 12 weeks-post implantation, significant remodeling activities are found and a new bone network is developed within the femoral defect. The 26-week samples show that the newly formed bone becomes more mature, while the interface between residual cement and the new bone appears less identifiable. Image analysis indicates that the resorption rate of the present cement is much higher than that of TTCP or TTCP/DCPA-derived cement under similar implantation conditions.
authors.affiliation: Department of Materials Science and Engineering, National Cheng-Kung University, 70101 Tainan City, Taiwan, ROC.
authors.forename: Wei-Luen
authors.initials: WL
authors.lastname: Chen
authors.affiliation: Department of Materials Science and Engineering, National Cheng-Kung University, 70101 Tainan City, Taiwan, ROC.
authors.forename: Chang-Keng
authors.initials: CK
authors.lastname: Chen
authors.affiliation: Section of Plastic Surgery, Department of Surgery, National Cheng-Kung University Medical College and Hospital, 70403 Tainan, Taiwan, ROC.
authors.forename: Jing-Wei
authors.initials: JW
authors.lastname: Lee
authors.affiliation: Department of Materials Science and Engineering, National Cheng-Kung University, 70101 Tainan City, Taiwan, ROC.
authors.forename: Yu-Ling
authors.initials: YL
authors.lastname: Lee
authors.affiliation: Department of Materials Science and Engineering, National Cheng-Kung University, 70101 Tainan City, Taiwan, ROC.
authors.forename: Chien-Ping
authors.initials: CP
authors.lastname: Ju
authors.affiliation: Department of Materials Science and Engineering, National Cheng-Kung University, 70101 Tainan City, Taiwan, ROC. Electronic address: chernlin9@gmail.com.
authors.forename: Jiin-Huey Chern
authors.initials: JH
authors.lastname: Lin
mesh_terms: D000818:Animals; D001843:Bone Cements / Q000737:chemistry* / Q000627:therapeutic use / Q000633:toxicity; D002130:Calcium Phosphates / Q000737:chemistry*; D002133:Calcium Sulfate / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D019245:Compressive Strength; D005264:Femoral Fractures / Q000473:pathology / Q000628:therapy; D005269:Femur / Q000473:pathology; D008297:Male; D051379:Mice; D041681:NIH 3T3 Cells; D016062:Porosity; D011817:Rabbits
chemical_list: D001843:Bone Cements; D002130:Calcium Phosphates; C485830:tetracalcium phosphate; C485829:calcium phosphate, dibasic, anhydrous; D002133:Calcium Sulfate
keywords: Animal study; Biphasic calcium based ceramic; Bone substitutes; Osteoconduction