title: Influence of needle gauge used for venipuncture on automated platelet count and coagulation profile in dogs.
abstract: OBJECTIVE
  To determine if differing gauge (G) needles used for venipuncture altered the automated platelet count and coagulation profile (prothrombin time (PT) and activated partial thromboplastin time (aPTT)) in clinically healthy dogs.
  
  
  METHODS
  Prospective, observational, randomised, clinical study.
  
  
  METHODS
  We enrolled 20 clinically healthy dogs. Blood was collected via direct venipuncture of the jugular veins with 21G, 23G and 25G needles in a random order. Automated haematology and automated coagulation times were performed on the blood samples. Values were analysed for differences among the needle gauges and also the order of sample collection.
  
  
  RESULTS
  No difference was found in the automated platelet count or automated coagulation times for the three needle gauges used or the order in which the samples were collected.
  
  
  CONCLUSIONS
  Venipuncture can be performed with a 21G, 23G or 25G needle to obtain blood from dogs for automated platelet count and PT/aPTT measurement without affecting the results.
authors.affiliation: Small Animal Specialist Hospital, 1 Richardson Place, North Ryde, New South Wales, 2213, Australia. cgreenwell@sashvets.com.
authors.forename: C M
authors.initials: CM
authors.lastname: Greenwell
authors.forename: S E
authors.initials: SE
authors.lastname: Epstein
authors.forename: P H
authors.initials: PH
authors.lastname: Brain
mesh_terms: D000818:Animals; D001779:Blood Coagulation Factors / Q000502:physiology*; D001792:Blood Platelets / Q000502:physiology*; D004285:Dogs / Q000097:blood*; D009339:Needles / Q000592:standards*; D018962:Phlebotomy / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D010976:Platelet Count / Q000379:methods / Q000662:veterinary
chemical_list: D001779:Blood Coagulation Factors
keywords: dogs; needle bore; needle size; partial thromboplastin time; prothrombin time