title: The effect of post-wash total progressive motile sperm count and semen volume on pregnancy outcomes in intrauterine insemination cycles: a retrospective study.
abstract: OBJECTIVE
  The purpose of this study was to determine the impact of post-wash total progressive motile sperm count (TPMSC) and semen volume on pregnancy outcomes in intrauterine insemination (IUI) cycles.
  
  
  METHODS
  The retrospective study included a total of 156 cycles (141 couples) and was performed in our center over a 24-month period. The semen parameters were recorded for each man and each insemination. The semen samples were re-evaluated after the preparation process. Post-wash TPMSC values were divided into four groups; Group 1: <1×10(6); Group 2: 1-4.9×10(6); Group 3: 5-9.9×10(6); Group 4: 10×10(6) and >10×10(6). Post-wash inseminated semen volume was divided into three groups; Group 1: 0.3 mL; Group 2: 0.4 mL; Group 3: 0.5 mL. The effect of post-wash total progressive motile sperm and semen volume on pregnancy outcomes was evaluated.
  
  
  RESULTS
  The pregnancy rates per cycle and per couple were 27.56% and 30.49%, respectively. There was not a significant relationship between the inseminated semen volume and pregnancy rate (p>0.05). However, a significant linear-by-linear association was documented between the TPMSC and pregnancy rate (p=0.042).
  
  
  CONCLUSIONS
  Our findings suggest that the post-wash inseminated semen volume should be between 0.3-0.5 mL. An average post-wash total motile sperm count of 10×10(6) may be a useful threshold value for IUI success, but more studies are needed to determine a cut-off value for TPMSC.
authors.affiliation: Division of Reproductive Medicine, Department of Obstetrics And Gynecology, Dokuz Eylül University Medical Faculty, İzmir, Turkey.
authors.forename: Elvan Koyun
authors.initials: EK
authors.lastname: Ok
authors.affiliation: Division of Reproductive Medicine, Department of Obstetrics And Gynecology, Dokuz Eylül University Medical Faculty, İzmir, Turkey.
authors.forename: Omer Erbil
authors.initials: OE
authors.lastname: Doğan
authors.affiliation: Division of Reproductive Medicine, Department of Obstetrics And Gynecology, Dokuz Eylül University Medical Faculty, İzmir, Turkey.
authors.forename: Recep Emre
authors.initials: RE
authors.lastname: Okyay
authors.affiliation: Division of Reproductive Medicine, Department of Obstetrics And Gynecology, Dokuz Eylül University Medical Faculty, İzmir, Turkey.
authors.forename: Bülent
authors.initials: B
authors.lastname: Gülekli
keywords: Intrauterine insemination; post-wash semen volume; post-wash total progressive motile sperm count; pregnancy