title: In-Vivo functional optical-resolution photoacoustic microscopy with stimulated Raman scattering fiber-laser source.
abstract: In this paper a multi-wavelength optical-resolution photoacoustic microscopy (OR-PAM) system using stimulated Raman scattering is demonstrated for both phantom and in vivo imaging. A 1-ns pulse width ytterbium-doped fiber laser is coupled into a single-mode polarization maintaining fiber. Discrete Raman-shifted wavelength peaks extending to nearly 800 nm are generated with pulse energies sufficient for OR-PAM imaging. Bandpass filters are used to select imaging wavelengths. A dual-mirror galvanometer system was used to scan the focused outputs across samples of carbon fiber networks, 200μm dye-filled tubes, and Swiss Webster mouse ears. Photoacoustic signals were collected in transmission mode and used to create maximum amplitude projection C-scan images. Double dye experiments and in vivo oxygen saturation estimation confirmed functional imaging potential.
authors.affiliation: Department of Electrical and Computer Engineering, University of Alberta, Edmonton, Alberta, T6G 2V4, Canada.
authors.forename: Parsin
authors.initials: P
authors.lastname: Hajireza
authors.affiliation: Department of Electrical and Computer Engineering, University of Alberta, Edmonton, Alberta, T6G 2V4, Canada.
authors.forename: Alexander
authors.initials: A
authors.lastname: Forbrich
authors.affiliation: Department of Electrical and Computer Engineering, University of Alberta, Edmonton, Alberta, T6G 2V4, Canada.
authors.forename: Roger
authors.initials: R
authors.lastname: Zemp
keywords: (110.5120) Photoacoustic imaging; (170.3880) Medical and biological imaging; (180.5810) Scanning microscopy; (290.5860) Scattering, Raman