title: Quantitative determination of active Bowman-Birk isoinhibitors, IBB1 and IBBD2, in commercial soymilks.
abstract: Naturally-occurring serine protease inhibitors of the Bowman-Birk family exert their potential chemopreventive and/or therapeutic properties via protease inhibition. In this study, we have quantified the amounts of active BBI isoinhibitors, IBB1 and IBBD2, in six commercial soymilks. By using cation exchange chromatography, the BBI isoinhibitors were isolated and their specific trypsin inhibitory activity was used to estimate their amounts in soymilk samples. IBB1 and IBBD2 concentrations ranged from 0.44 to 5.20 and 0.27 to 4.60 mg/100ml of soymilk, respectively; total BBI, considered as the sum of both isoinhibitors, ranged from 0.60 to 9.07 mg/100ml of soymilk. These data show that physiologically relevant amounts of active BBI are present in commercial soymilk and may exert potential health-promoting effects.
authors.affiliation: Department of Nutrition, Estación Experimental del Zaidín (CSIC), Profesor Albareda 1, 18008 Granada, Spain.
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Arques
authors.affiliation: Department of Nutrition, Estación Experimental del Zaidín (CSIC), Profesor Albareda 1, 18008 Granada, Spain.
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Marín-Manzano
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal do Ceará, Fortaleza, Brazil.
authors.forename: L Clarissa Brito
authors.initials: LC
authors.lastname: da Rocha
authors.affiliation: Department of Food Analysis and Bioactivity, Institute of Food Science (CIAL, CSIC-UAM), Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: Blanca
authors.initials: B
authors.lastname: Hernandez-Ledesma
authors.affiliation: Department of Food Analysis and Bioactivity, Institute of Food Science (CIAL, CSIC-UAM), Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: Isidra
authors.initials: I
authors.lastname: Recio
authors.affiliation: Department of Nutrition, Estación Experimental del Zaidín (CSIC), Profesor Albareda 1, 18008 Granada, Spain. Electronic address: alfonso.clemente@eez.csic.es.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Clemente
mesh_terms: D010936:Plant Extracts / Q000032:analysis* / Q000302:isolation & purification; D041942:Soy Milk / Q000737:chemistry* / Q000191:economics; D013025:Glycine max / Q000737:chemistry*; D014358:Trypsin Inhibitor, Bowman-Birk Soybean / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D010936:Plant Extracts; D014358:Trypsin Inhibitor, Bowman-Birk Soybean
keywords: Bowman-Birk inhibitors; Chemoprevention; Kunitz; Protease inhibitors; Serine proteases; Soymilk