title: Dereplication of microbial extracts and related analytical technologies.
abstract: Natural products still continue to have an important role as a resource of various biologically active substances. Dereplication is a key process in natural product screening that analyzes the extracts of microbial fermentation broths or plant samples. In this review article, we describe and discuss the analytical techniques of dereplication and related technologies in the following sections: 1. Direct detection from microbial colonies. 2. Ultra high performance liquid chromatography (UHPLC)-MS profiling for library construction. 3. Micro-fractionation to identify active peaks. 4. Quantification of small-amount compounds. 5. Structure identification from small amounts. Using these techniques, the desired compound in the mixture library can be rapidly identified.
authors.affiliation: Research Division, Chugai Pharmaceutical Co., Ltd., Kamakura, Kanagawa, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Ito
authors.affiliation: Research Division, Chugai Pharmaceutical Co., Ltd., Kamakura, Kanagawa, Japan.
authors.forename: Miyako
authors.initials: M
authors.lastname: Masubuchi
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D001419:Bacteria / Q000737:chemistry; D001688:Biological Products / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004261:DNA Replication; D005285:Fermentation; D005658:Fungi / Q000737:chemistry; D015723:Gene Library; D057166:High-Throughput Screening Assays; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry
chemical_list: D000900:Anti-Bacterial Agents; D001688:Biological Products