title: Production of a biodegradable plastic-degrading enzyme from cheese whey by the phyllosphere yeast Pseudozyma antarctica GB-4(1)W.
abstract: Cheese whey is a by-product of cheese production and has high concentrations of lactose (about 5%) and other nutrients. Pseudozyma antarctica produces a unique cutinase-like enzyme, named PaE, that efficiently degrades biodegradable plastics. A previous study showed that a combination of 1% oil and 0.5% lactose increased cutinase-like enzyme production by another species of yeast. In this study, to produce PaE from cheese whey, we investigated the effects of soybean oil on PaE production (expressed as biodegradable plastic-degrading activity) by P. antarctica growing on lactose or cheese whey. In flask cultures, the final PaE activity was only 0.03 U/ml when soybean oil was used as the sole carbon source, but increased to 1.79 U/ml when a limited amount of soybean oil (under 0.5%) was combined with a relatively high concentration of lactose (6%). Using a 5-L jar fermentor with lactose fed-batch cultivation and periodic soybean oil addition, about 14.6 U/ml of PaE was obtained after 5 days of cultivation. When the lactose was replaced with cheese whey, PaE production was 10.8 U/ml after 3 days of cultivation.
authors.affiliation: National Institute for Agro-Environmental Sciences (NIAES), 3-1-3 Kannondai, Tsukuba, Ibaraki 305-8604, Japan; The Japan Society for the Promotion of Science, 1-8 Chiyoda-ku, Tokyo 102-8472, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Watanabe
authors.affiliation: National Institute for Agro-Environmental Sciences (NIAES), 3-1-3 Kannondai, Tsukuba, Ibaraki 305-8604, Japan; The Japan Society for the Promotion of Science, 1-8 Chiyoda-ku, Tokyo 102-8472, Japan.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Shinozaki
authors.affiliation: National Institute for Agro-Environmental Sciences (NIAES), 3-1-3 Kannondai, Tsukuba, Ibaraki 305-8604, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Suzuki
authors.affiliation: National Institute for Agro-Environmental Sciences (NIAES), 3-1-3 Kannondai, Tsukuba, Ibaraki 305-8604, Japan.
authors.forename: Motoo
authors.initials: M
authors.lastname: Koitabashi
authors.affiliation: National Institute for Agro-Environmental Sciences (NIAES), 3-1-3 Kannondai, Tsukuba, Ibaraki 305-8604, Japan.
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Yoshida
authors.affiliation: National Institute for Agro-Environmental Sciences (NIAES), 3-1-3 Kannondai, Tsukuba, Ibaraki 305-8604, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Sameshima-Yamashita
authors.affiliation: National Institute for Agro-Environmental Sciences (NIAES), 3-1-3 Kannondai, Tsukuba, Ibaraki 305-8604, Japan. Electronic address: kitamoto@affrc.go.jp.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Kuze Kitamoto
mesh_terms: D063566:Biodegradable Plastics; D019149:Bioreactors; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis*; D002611:Cheese; D003470:Culture Media; D005656:Fungal Proteins / Q000096:biosynthesis*; D007785:Lactose / Q000737:chemistry; D013024:Soybean Oil / Q000737:chemistry; D014587:Ustilaginales / Q000201:enzymology*
chemical_list: D063566:Biodegradable Plastics; D003470:Culture Media; D005656:Fungal Proteins; D013024:Soybean Oil; D002265:Carboxylic Ester Hydrolases; C019952:cutinase; D007785:Lactose
keywords: Biodegradable plastics; Cheese whey; Cutinase-like enzyme; Jar fermentor; Phyllosphere yeast; Pseudozyma antarctica