title: Evaluation of new immunochromatographic assay kit for adenovirus detection in throat swab: comparison with culture and real-time PCR results.
abstract: A new immunochromatographic (IC) assay kit, BD Veritor System Adeno was evaluated to comparing with commercial available kit, BD Adeno Examan, cell culture, and real-time PCR using throat swab samples. Specimens were collected from 146 pediatric patients between July 2011 and January 2012. Mean age of patients was 4 years (8 months-15 years old). Patients were diagnosed with pharyngitis (n = 67), tonsillitis (n = 45), pharyngoconjunctival fever (n = 26), upper respiratory tract infection (n = 6), conjunctivitis (n = 1), or bronchitis (n = 1). Thirty-one of the patients (21.2%) had more than one disease. Among all samples, 61 (41.8%) were positive for adenovirus with BD Veritor System Adeno; 68 (46.6%) with BD Adeno Examan; 63 (43.2%) with real-time PCR; and 65 (44.5%) with cell culture. Serotype 3 (n = 41; 63.1%) was predominant among the 65 adenovirus isolates, followed by serotype 2 (n = 12; 18.5%), 1 (n = 6; 9.2%), 5 (n = 4; 6.2%), and 4 (n = 2; 3.1%). Relative sensitivity and specificity of BD Veritor System Adeno, BD Adeno Examan, and real-time PCR were 93.8% and 98.7%, 96.9% and 93.8%, and 96.9% and 100%, respectively. Positive predictive and negative predictive values for these methods were 98.4% and 95.1%, 92.6% and 97.4%, and 100% and 97.6%, respectively. The sensitivity and specificity of real-time PCR was greater than that of IC assay kits. However, IC assay kits also showed high sensitivity and specificity appropriate for clinical use.
authors.affiliation: Laboratory of Molecular Epidemiology for Infectious Agents, Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan; Department of Infectious Diseases, Keio University School of Medicine, Tokyo, Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Morozumi
authors.affiliation: Division of Virology, Kawasaki City Institute for Public Health, Kanagawa, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Shimizu
authors.affiliation: Division of Virology, Kawasaki City Institute for Public Health, Kanagawa, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Matsushima
authors.affiliation: Department of Pediatrics, Eiju General Hospital, Tokyo, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Mitamura
authors.affiliation: Department of Pediatrics, Hakujikai Memorial Hospital, Tokyo, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tajima
authors.affiliation: Department of Infectious Diseases, Keio University School of Medicine, Tokyo, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Iwata
authors.affiliation: Laboratory of Molecular Epidemiology for Infectious Agents, Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan; Department of Infectious Diseases, Keio University School of Medicine, Tokyo, Japan. Electronic address: ubukatak@a6.keio.jp.
authors.forename: Kimiko
authors.initials: K
authors.lastname: Ubukata
mesh_terms: D000260:Adenoviruses, Human / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D010614:Pharynx / Q000821:virology*; D011933:Reagent Kits, Diagnostic; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
chemical_list: D011933:Reagent Kits, Diagnostic
keywords: Adenovirus; Immunochromatographic assay; Rapid detection; Real-time PCR; Serotype