title: Morphological good-quality embryo has higher nucleus spreading rate/signal resolution rate in fluorescence in situ hybridization.
abstract: OBJECTIVE
  To evaluate the relationship between day 3 embryo quality and nucleus spreading rate/signal resolution rate in Fluorescence in situ hybridization (FISH) during the PGD procedure.
  
  
  METHODS
  This study was a retrospective data analysis. 367 day-3 embryos were classified based on morphological scoring: grade 1 to grade 4 were defined from worse to better embryo quality. Day 3 embryos were classified as good quality when the number of blastomeres was between 6 and 10 and grade better than 2'. Nucleus spreading rate, signal rate and the full signal rate were compared between embryos with different morphological scoring.
  
  
  RESULTS
  Nucleus spreading rate of blastomeres from morphological high-quality embryos was significantly higher (86.25 %) than from poor-quality embryos (76.53 %) (p < 0.05). The rate of blastomeres with full signals was significantly higher (79.32 %) in the morphological high-quality group than in poor-quality group (64.54 %) (p < 0.05). Similar results were found from day 3 embryos with cell number between 6 cells and 10 cells (nucleus spreading rate 86.01 vs. 76.34 %, p < 0.05; full signal rate 78.72 vs. 62.71 %, p < 0.05). Both have no significant difference in the signal rate (82.67 vs. 89.66 %; 83.10 vs. 89.95 %).
  
  
  CONCLUSIONS
  Blastomeres from day 3 embryos with better morphological quality had higher nucleus spreading rate and higher full signal rate during FISH. Through this study, we speculate on whether it should reconsider the necessity of FISH application in embryos with poor quality.
authors.affiliation: Centre for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan, 250021, China.
authors.forename: Keliang
authors.initials: K
authors.lastname: Wu
authors.forename: Ye
authors.initials: Y
authors.lastname: Zheng
authors.forename: Yueting
authors.initials: Y
authors.lastname: Zhu
authors.forename: Hongchang
authors.initials: H
authors.lastname: Li
authors.forename: Guanling
authors.initials: G
authors.lastname: Yu
authors.forename: Junhao
authors.initials: J
authors.lastname: Yan
authors.forename: Zi-Jiang
authors.initials: ZJ
authors.lastname: Chen
mesh_terms: D001757:Blastomeres / Q000166:cytology* / Q000502:physiology; D002467:Cell Nucleus; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D053652:Nuclear Transfer Techniques; D011247:Pregnancy; D018873:Pregnancy Rate; D019836:Preimplantation Diagnosis / Q000379:methods*; D012189:Retrospective Studies