title: Immunohistochemical characterization of non-epithelial cells in spiradenoma.
abstract: Spiradenoma is unique with respect to the presence of a large number of non-epithelial cells, including S100 protein(+) cells, most of which are presumably Langerhans cells, in the parenchyma as shown in the published work. However, the characterization of these non-epithelial cells to date is insufficient. Immunohistochemistry of CD1a, CD3, CD4, CD8, CD56, CD68, intercellular adhesion molecule-1 (ICAM-1), and HLA-DR, as well as double-immunofluorescence labeling of S100 protein/CD1a and CD1a/CD3, was performed using paraffin-embedded specimens from five cases of spiradenoma retrospectively. Non-epithelial cells evenly distributed throughout the parenchyma of spiradenoma primarily consisted of CD1a(+) Langerhans cells and CD3(+) T cells. ICAM-1 was expressed by epithelial cells and non-epithelial cells in the parenchyma. HLA-DR on the epithelial cells was limited to the focal area. In double-immunofluorescence labeling, approximately one-half of Langerhans cells were spatially related to T cells in the parenchyma, suggesting their functional interaction.
authors.affiliation: Department of Dermatology, Showa University School of Medicine, Tokyo.
authors.forename: Kohji
authors.initials: K
authors.lastname: Iida
authors.forename: Shinsaku
authors.initials: S
authors.lastname: Iwai
authors.forename: Hiroomi
authors.initials: H
authors.lastname: Hosaka
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kitami
authors.forename: Masaki
authors.initials: M
authors.lastname: Akiyama
authors.forename: Takao
authors.initials: T
authors.lastname: Suzuki
authors.forename: Hirohiko
authors.initials: H
authors.lastname: Sueki
mesh_terms: D000236:Adenoma / Q000276:immunology / Q000473:pathology*; D000328:Adult; D000368:Aged; D018949:Antigens, CD1 / Q000032:analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007801:Langerhans Cells / Q000737:chemistry / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D012867:Skin / Q000276:immunology / Q000473:pathology; D012878:Skin Neoplasms / Q000276:immunology / Q000473:pathology*; D013601:T-Lymphocytes / Q000473:pathology*
chemical_list: D018949:Antigens, CD1; C419393:CD1a antigen
keywords: Langerhans cells; T lymphocytes; spiradenoma