title: Protective effect of secondary plant metabolites from Ipomoea aquatica Forsk. against carbofuran induced damages.
abstract: Plausible interactions between food contaminants and natural constituents in vivo and protective effect of polyphenols present in I. aquatica against carbofuran toxicity in Charles Foster rats were evaluated. Determinations based on antioxidant enzyme activities showed significant alterations in glutathione, glutathione peroxidase, superoxide dismutase and catalase in tissues (liver and brain) and plasma of pesticide treated group while polyphenolic extracts from I. aquatica (IAE) attenuated their activities when given alongwith carbofuran. IAE decreased enhanced lipid peroxidation levels in plasma and erythrocyte membrane and cholesterol levels in brain and plasma. IAE also minimized histopathological degenerative changes produced by carbofuran. While single cell gel electrophoresis showed that secondary metabolites in leafy vegetables produced a combinatorial effect with pesticide at cellular level, DNA fragmentation level in bone marrow cells showed a decline in the IAE treated rats. Food safety adversely affected by various chemical contaminants can be retained by plant polyphenols and secondary plant constituents that can be found together in bolus. Therefore, the present study gives an insight into the protective role of naturally found polyphenols against pesticide toxicity.
authors.affiliation: Department of Chemical Technology, University College of Science and Technology, University of Calcutta, 92, A.P.C. Road, Kolkata 700 009, India. sanj_9231@yahoo.co.in
authors.forename: Sanjukta
authors.initials: S
authors.lastname: Datta
authors.affiliation: Department of Physiology, University College of Science and Technology, University of Calcutta, 92, A.P.C. Road, Kolkata 700 009, India.
authors.forename: Mahuya
authors.initials: M
authors.lastname: Sinha
authors.affiliation: Department of Physiology, University College of Science and Technology, University of Calcutta, 92, A.P.C. Road, Kolkata 700 009, India.
authors.forename: Dipesh
authors.initials: D
authors.lastname: Das
authors.affiliation: Department of Chemical Technology, University College of Science and Technology, University of Calcutta, 92, A.P.C. Road, Kolkata 700 009, India.
authors.forename: Santinath
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Laboratory of Food Science and Tecnology, Food and Nutrition Division, Department of Home Science, University of Calcutta, 20 B Judges Court Road, Kolkata 700 027, India.
authors.forename: Pubali
authors.initials: P
authors.lastname: Dhar
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry; D002235:Carbofuran / Q000633:toxicity; D002374:Catalase / Q000097:blood; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000188:drug therapy* / Q000201:enzymology; D005979:Glutathione Peroxidase / Q000097:blood; D027741:Ipomoea / Q000737:chemistry*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D059808:Polyphenols / Q000008:administration & dosage / Q000737:chemistry; D051381:Rats; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D059808:Polyphenols; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D002235:Carbofuran