title: The proteins of the grape (Vitis vinifera L.) seed endosperm: fractionation and identification of the major components.
abstract: In the present study, grape (Vitis vinifera L.) seed endosperm proteins were characterized after sequential fractionation, according to a modified Osborne procedure. The salt-soluble fraction (albumins and globulins) comprised the majority (58.4%) of the total extracted protein. The protein fractions analysed by SDS-PAGE showed similar bands, indicating different solubility of the same protein components. SDS-PAGE in non-reducing and reducing conditions revealed the polypeptide composition of the protein bands. The main polypeptides, which were similar in all the grape varieties analysed, were identified by LC-MS/MS as homologous to the 11S globulin-like seed storage proteins of other plant species, while a monomeric 43 kDa protein presented high homology with the 7S globulins of legume seeds. The results provide new insights about the identity, structure and polypeptide composition of the grape seed storage proteins.
authors.affiliation: Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE) and Centro Interdipartimentale per la Ricerca in Viticoltura ed Enologia (CIRVE), Padova University, via dell'Università 16, 35020 Legnaro (PD), Italy.
authors.forename: Diana
authors.initials: D
authors.lastname: Gazzola
authors.affiliation: Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE) and Centro Interdipartimentale per la Ricerca in Viticoltura ed Enologia (CIRVE), Padova University, via dell'Università 16, 35020 Legnaro (PD), Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Vincenzi
authors.affiliation: Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE) and Centro Interdipartimentale per la Ricerca in Viticoltura ed Enologia (CIRVE), Padova University, via dell'Università 16, 35020 Legnaro (PD), Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Gastaldon
authors.affiliation: Proteomics Center of Padova University, VIMM and Padova University Hospital, via G. Orus 2b, 35129 Padova, Italy.
authors.forename: Serena
authors.initials: S
authors.lastname: Tolin
authors.affiliation: Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE) and Centro Interdipartimentale per la Ricerca in Viticoltura ed Enologia (CIRVE), Padova University, via dell'Università 16, 35020 Legnaro (PD), Italy.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Pasini
authors.affiliation: Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE) and Centro Interdipartimentale per la Ricerca in Viticoltura ed Enologia (CIRVE), Padova University, via dell'Università 16, 35020 Legnaro (PD), Italy. Electronic address: andrea.curioni@unipd.it.
authors.forename: Andrea
authors.initials: A
authors.lastname: Curioni
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D056625:Endosperm / Q000737:chemistry*; D005916:Globulins / Q000737:chemistry / Q000302:isolation & purification; D013058:Mass Spectrometry; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D055314:Seed Storage Proteins / Q000737:chemistry / Q000302:isolation & purification; D012639:Seeds / Q000737:chemistry*; D027843:Vitis / Q000737:chemistry*
chemical_list: D005916:Globulins; D010940:Plant Proteins; D055314:Seed Storage Proteins
keywords: Electrophoresis; Globulins; Grape seed; Mass spectrometry; Seed proteins