title: Chromium distribution in shoots of macrophyte Callitriche cophocarpa Sendtn.
abstract: The aim of the study was the analysis of Cr distribution in shoots of the macrophyte Callitriche cophocarpa by means of two X-ray-based techniques: micro X-ray fluorescence (μXRF) and electron probe X-ray microanalysis (EPXMA). Plants were treated with 100 μM (5.2 mg l(-1)) chromium solutions for 7 days. Cr was introduced independently at two speciations as Cr(III) and Cr(VI), known for their diverse physicochemical properties and different influence on living organisms. A comparative analysis of Cr(III)-treated plants by EPXMA and μXRF demonstrated high deposition of Cr in epidermal glands/hairs localized on leaves and stems of the plant shoots. Cr in Cr(III)-treated plants was recorded solely in glands/hairs, and the element was not present in any other structures. On the other hand, Cr in Cr(VI)-treated group of plants was rather found in vascular bundles. Moreover, the concentration of Cr in Cr(VI)-treated plants was significantly lower than in plants incubated in Cr(III) solution. The results obtained in this work suggest differences in chromium uptake, transport and accumulation dependent on the oxidative state of the element.
authors.affiliation: Unit of Botany and Plant Physiology, Faculty of Horticulture, Institute of Plant Biology and Biotechnology, University of Agriculture in Kraków, al. 29 Listopada 54, 31-425, Kraków, Poland, augustyn@ogr.ur.krakow.pl.
authors.forename: Joanna
authors.initials: J
authors.lastname: Augustynowicz
authors.forename: Paweł
authors.initials: P
authors.lastname: Wróbel
authors.forename: Bartosz J
authors.initials: BJ
authors.lastname: Płachno
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Tylko
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Gajewski
authors.forename: Dariusz
authors.initials: D
authors.lastname: Węgrzynek
mesh_terms: D000818:Animals; D002857:Chromium / Q000737:chemistry / Q000378:metabolism*; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D018520:Plant Shoots / Q000737:chemistry / Q000378:metabolism*; D018547:Plant Stems / Q000737:chemistry / Q000378:metabolism; D010944:Plants / Q000378:metabolism*
chemical_list: D002857:Chromium