title: Identification of novel binding partners for caspase-6 using a proteomic approach.
abstract: Apoptosis is the process of programmed cell death executed by specific proteases, the caspases, which mediate the cleavage of various vital proteins. Elucidating the consequences of this endoproteolytic cleavage is crucial to understanding cell death and other related biological processes. Although a number of possible roles for caspase-6 have been proposed, the identities and functions of proteins that interact with caspase-6 remain uncertain. In this study, we established a cell line expressing tandem affinity purification (TAP)-tagged caspase- 6 and then used LC-MS/MS proteomic analysis to analyze the caspase-6 interactome. Eight candidate caspase-6-interacting proteins were identified. Of these, five proteins (hnRNP-M, DHX38, ASPP2, MTA2, and UACA) were subsequently examined by co-immunoprecipitation for interactions with caspase-6. Thus, we identified two novel members of the caspase-6 interactome: hnRNP-M and MTA2.
authors.affiliation: Medical Proeomics Research Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon 305-333, Republic of Korea, Department of Biology, Kongju National University, Kongju 314-701, Republic of Korea.
authors.forename: Ju Yeon
authors.initials: JY
authors.lastname: Jung
authors.forename: Su Rim
authors.initials: SR
authors.lastname: Lee
authors.forename: Sunhong
authors.initials: S
authors.lastname: Kim
authors.forename: Seung Wook
authors.initials: SW
authors.lastname: Chi
authors.forename: Kwang-Hee
authors.initials: KH
authors.lastname: Bae
authors.forename: Byoung Chul
authors.initials: BC
authors.lastname: Park
authors.forename: Jeong-Hoon
authors.initials: JH
authors.lastname: Kim
authors.forename: Sung Goo
authors.initials: SG
authors.lastname: Park
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism*; D053178:Caspase 6 / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D011485:Protein Binding; D025941:Protein Interaction Mapping; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results
chemical_list: D002352:Carrier Proteins; D053178:Caspase 6