title: Label-free electrochemical immunosensor based on gold-silicon carbide nanocomposites for sensitive detection of human chorionic gonadotrophin.
abstract: Uniform and highly dispersed gold-silicon carbide (Au@SiC) nanocomposites were prepared via simple way and used for fabrication of label-free electrochemical immunosensor for sensitive detection of human chorionic gonadotrophin (hCG). Using Au@SiC as electrode material and using ferricyanide as mediator, the proposed immunosensor provides a simple and economic method with higher sensitivity and a wider concentration range for detection of hCG. Under the optimal condition, the approach provided a good linear response range from 0.1 to 5 IU/L and 5 to 1000 IU/L with a low detection limit of 0.042 IU/L. The immunosensor showed good selectivity, acceptable stability and reproducibility. Satisfactory results were obtained for determination of hCG in human serum samples. The proposed method provides a promising platform of clinical immunoassay for other biomolecules. In addition, the bio-functionalization of SiC combined with other nanomaterials will provide promising approach for electrochemical sensing and biosensing platform.
authors.affiliation: School of Chemical Science and Technology, Yunnan University, Kunming 650091, PR China.
authors.forename: Long
authors.initials: L
authors.lastname: Yang
authors.affiliation: School of Life Science, Yunnan University, Kunming 650091, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.affiliation: School of Chemical Science and Technology, Yunnan University, Kunming 650091, PR China.
authors.forename: Shuangmei
authors.initials: S
authors.lastname: Fan
authors.affiliation: School of Life Science, Yunnan University, Kunming 650091, PR China.
authors.forename: Shuangsheng
authors.initials: S
authors.lastname: Deng
authors.affiliation: School of Life Science, Yunnan University, Kunming 650091, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Lv
authors.affiliation: School of Life Science, Yunnan University, Kunming 650091, PR China. Electronic address: jielinynu@126.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Lin
authors.affiliation: School of Chemical Science and Technology, Yunnan University, Kunming 650091, PR China. Electronic address: lcppp1974@sina.com.
authors.forename: Can-Peng
authors.initials: CP
authors.lastname: Li
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D017554:Carbon Compounds, Inorganic / Q000737:chemistry*; D006063:Chorionic Gonadotropin / Q000097:blood*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation; D057230:Limit of Detection; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results; D017655:Silicon Compounds / Q000737:chemistry*
chemical_list: D017554:Carbon Compounds, Inorganic; D006063:Chorionic Gonadotropin; D017655:Silicon Compounds; D006046:Gold; C022088:silicon carbide
keywords: Electrochemical immunosensor; Gold–silicon carbide nanocomposite; Human chorionic gonadotrophin; Label-free