title: Apoptosis-associated genes related to photodynamic therapy in breast carcinomas.
abstract: The aim of this study was to find the apoptosis molecular markers involved in the cell death that might be related to photodynamic therapy (PDT) mechanisms in breast cancer. The mammary tumors were induced in 25 Sprague-Dawley female rats by a single, oral gavage of 7,12-dimethylbenz(a)anthracene (DMBA; 70 mg/kg body weight). Animals were divided into four groups: G1 (normal, without DMBA), G2 (control, without PDT treatment), G3 (euthanized 48 h after PDT), and G4 (euthanized 24 h after PDT). For PDT experiments, the photosensitizer used was Photodithazine, and 100 J/cm of light at a fluence rate of 100 mW/cm was delivered to treat lesions. A sample of each animal was investigated by quantitative real-time PCR using Rat Apoptosis RT2 Profiler™ PCR Array platform. The results showed 20 genes with differential expression between PDT and control groups. A significant upregulation was observed for pro-apoptotic genes CASP4, CASP12, CIDEA, GADD45A, and FAS and downregulation of anti-apoptotic genes MAPK8IP1, TNFRSF11B, and NAIP2 in PDT-treated tumors. These results indicate that these genes are more directly involved in cell apoptosis induced by PDT.
authors.affiliation: Laboratório de Espectroscopia Vibracional Biomédica, Instituto de Pesquisa e Desenvolvimento-IP&D, Universidade do Vale do Paraíba-UNIVAP, Avenida Shishima Hifumi 2911, São José dos Campos, SP, 12244-000, Brazil.
authors.forename: J C
authors.initials: JC
authors.lastname: Silva
authors.forename: J
authors.initials: J
authors.lastname: Ferreira-Strixino
authors.forename: L C
authors.initials: LC
authors.lastname: Fontana
authors.forename: L M
authors.initials: LM
authors.lastname: Paula
authors.forename: L
authors.initials: L
authors.lastname: Raniero
authors.forename: A A
authors.initials: AA
authors.lastname: Martin
authors.forename: R A
authors.initials: RA
authors.lastname: Canevari
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D051017:Apoptosis Regulatory Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D005944:Glucosamine / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D054023:Lasers, Semiconductor; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000188:drug therapy*; D010778:Photochemotherapy / Q000379:methods; D017319:Photosensitizing Agents / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D000970:Antineoplastic Agents; D051017:Apoptosis Regulatory Proteins; D017319:Photosensitizing Agents; C047635:N-methylglucosamine; D005944:Glucosamine