title: Role of type I- and type II-interferon in expression of melanoma differentiation-associated gene-5 in HSC-3 oral squamous carcinoma cells.
abstract: Melanoma differentiation-associated gene 5 (MDA-5) and retinoic acid-inducible gene-I (RIG-I)are members of DExH family of proteins, and known to play important roles in antiviral responses to induce type I interferons (IFNs). MDA-5 has been thought to sense RNA virus with long(>1 kb) double-stranded RNA. However, MDA-5 is also induced by type II IFN that is involved in acquired immunity, suggesting that role of MDA-5 remains to be elucidated. In addition, no study regarding MDA-5 in oral region has been performed. Here we investigated the role of MDA-5 in HCS-3 squamous carcinoma cells derived from oral epithelial cells. Treatment of HCS-3 cells with IFN-α2b or IFN-γ significantly induced MDA-5 as well as RIG-I. IFN-α2b exerted anti-proliferative effect in HSC-3 cells while no such effect was observed in the cells treated with IFN-γ. MDA-5 is known to be associated with tumor cell growth in melanoma. However, overexpression of MDA-5 did not alter the proliferation in HSC-3 cells, indicating that MDA-5 is unrelated to the cell growth in this type of cells. We conclude that MDA-5 is induced by both type I- and type II-IFNs in HSC-3 cells, and this suggests MDA-5 may play a role in immune responses in oral cavity.
authors.affiliation: Department ofVascular Biology, Institute of Brain Science, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.
authors.forename: Tomoh
authors.initials: T
authors.lastname: Matsumiya
authors.forename: Ryo
authors.initials: R
authors.lastname: Hayakari
authors.forename: Norihiko
authors.initials: N
authors.lastname: Narita
authors.forename: Ryohei
authors.initials: R
authors.lastname: Ito
authors.forename: Takao
authors.initials: T
authors.lastname: Kon
authors.forename: Kosei
authors.initials: K
authors.lastname: Kubota
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Sakaki
authors.forename: Hidemi
authors.initials: H
authors.lastname: Yoshida
authors.forename: Tadaatsu
authors.initials: T
authors.lastname: Imaizumi
authors.forename: Wataru
authors.initials: W
authors.lastname: Kobayashi
authors.forename: Hiroto
authors.initials: H
authors.lastname: Kimura
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D053487:DEAD-box RNA Helicases / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D000072640:Interferon-Induced Helicase, IFIH1; D007371:Interferon-gamma / Q000494:pharmacology*; D009062:Mouth Neoplasms / Q000235:genetics*
chemical_list: D007370:Interferon Type I; D007371:Interferon-gamma; C452815:IFIH1 protein, human; D053487:DEAD-box RNA Helicases; D000072640:Interferon-Induced Helicase, IFIH1