title: [Study of the role of miRNA in mesenchymal stem cells isolated from osteoarthritis patients].
abstract: OBJECTIVE
  MiRNAs act as gene silencers that are involved in the regulation of essential cell functions. miR-335 is involved in regulating cell differentiation processes in progenitor cells. Mesenchymal stem cells (MSCs) are progenitor cells of chondrocytes and osteoblasts responsible for homeostatic maintenance of cartilage and bone. The aim of this study was to determine a possible relationship between the expression of miR-335 and osteoarthritis.
  
  
  METHODS
  MSCs obtained from the bone marrow of 3 osteoarthritic patients and 3 controls with no clinical signs of osteoarthritis or osteoporosis were cultured and phenotypically and functionally characterised in a 3-step culture. Expression levels of miR-335 and the mesoderm-specific transcript gene -MEST- that controls its expression were determined by quantitative PCR.
  
  
  RESULTS
  Differences in the expression levels of miR-335 and MEST (median [interquartile range]: 1.69 [0.85-1.74], and 3.85 [3.20-5.67] were detected between MSCs isolated from patients with osteoarthritis and controls. Although the differences detected did not reach statistical significance (P=.1), a clear trend towards lower expression of miR-335 in osteoarthritis MSCs was observed.
  
  
  CONCLUSIONS
  Given that miR-335 has the different genes involved in the Wnt signalling pathway as potential targets, the observed trend may help to ascertain, at least partially, some of the alterations which determine the onset or progression of osteoarthritis, and can therefore serve for the design of future therapeutic targets for the treatment of this disease.
authors.affiliation: Unidad de Gestión Clínica de Reumatología, Hospital Clínico San Carlos, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), Madrid, España. Electronic address: mptornero@gmail.com.
authors.forename: P
authors.initials: P
authors.lastname: Tornero-Esteban
authors.affiliation: Unidad de Gestión Clínica de Reumatología, Hospital Clínico San Carlos, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), Madrid, España.
authors.forename: J A
authors.initials: JA
authors.lastname: Hoyas
authors.affiliation: Unidad de Gestión Clínica de Reumatología, Hospital Clínico San Carlos, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), Madrid, España.
authors.forename: E
authors.initials: E
authors.lastname: Villafuertes
authors.affiliation: Unidad de Gestión Clínica de Traumatología, Hospital Clínico San Carlos, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), Madrid, España.
authors.forename: I
authors.initials: I
authors.lastname: Garcia-Bullón
authors.affiliation: Unidad de Gestión Clínica de Traumatología, Hospital Clínico San Carlos, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), Madrid, España.
authors.forename: E
authors.initials: E
authors.lastname: Moro
authors.affiliation: Unidad de Gestión Clínica de Reumatología, Hospital Clínico San Carlos, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), Madrid, España.
authors.forename: B
authors.initials: B
authors.lastname: Fernández-Gutiérrez
authors.affiliation: Unidad de Gestión Clínica de Traumatología, Hospital Clínico San Carlos, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), Madrid, España.
authors.forename: F
authors.initials: F
authors.lastname: Marco
mesh_terms: D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D035683:MicroRNAs / Q000502:physiology*; D010003:Osteoarthritis / Q000209:etiology / Q000473:pathology*
chemical_list: C556347:MIRN335 microRNA, human; D035683:MicroRNAs
keywords: Artrosis; Células madre mesenquimales; Mesenchymal stem cells; Microribonucleic acids; Microácidos ribonucleicos; Osteoarthritis