title: Comparative study of the immunogenicity in mice and monkeys of an inactivated CA16 vaccine made from a human diploid cell line.
abstract: The coxsackie A16 virus (CA16), along with enterovirus 71 (EV71), is a primary pathogen that causes hand, foot, and mouth disease (HFMD). To control HFMD, CA16, and EV71 vaccines are needed. In this study, an experimental inactivated CA16 vaccine was prepared using human diploid cells, and the vaccine's immunogenicity was analyzed in mice and rhesus monkeys. The results showed that the neutralizing antibody was developed in a dose-dependent manner, and was sustained for 70 days with an average GMT (geometric mean titer) level of 80 to 90 in immunized mouse and for 56 days with GMT of higher than 300 in monkeys. The neutralizing antibody had a cross-neutralizing activity against different viral strains (genotype A and B), and the specific IFN-γ-secreting cell response was activated by these virus strains in an ELISPOT assay. This study provides evidence for the potential use of inactivated CA16 as a candidate for use in vaccines.
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases; Institute of Medical Biology; Chinese Academy of Medicine Sciences & Peking Union Medical College; Kunming, PR China; Jiangsu Convac Biotechnology Co., Ltd; Taizhou Jiangsu, PR China.
authors.forename: Erxia
authors.initials: E
authors.lastname: Yang
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases; Institute of Medical Biology; Chinese Academy of Medicine Sciences & Peking Union Medical College; Kunming, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Cheng
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases; Institute of Medical Biology; Chinese Academy of Medicine Sciences & Peking Union Medical College; Kunming, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases; Institute of Medical Biology; Chinese Academy of Medicine Sciences & Peking Union Medical College; Kunming, PR China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases; Institute of Medical Biology; Chinese Academy of Medicine Sciences & Peking Union Medical College; Kunming, PR China.
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Che
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases; Institute of Medical Biology; Chinese Academy of Medicine Sciences & Peking Union Medical College; Kunming, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Pu
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases; Institute of Medical Biology; Chinese Academy of Medicine Sciences & Peking Union Medical College; Kunming, PR China.
authors.forename: Chenghong
authors.initials: C
authors.lastname: Dong
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases; Institute of Medical Biology; Chinese Academy of Medicine Sciences & Peking Union Medical College; Kunming, PR China.
authors.forename: Longding
authors.initials: L
authors.lastname: Liu
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases; Institute of Medical Biology; Chinese Academy of Medicine Sciences & Peking Union Medical College; Kunming, PR China.
authors.forename: Zhanlong
authors.initials: Z
authors.lastname: He
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases; Institute of Medical Biology; Chinese Academy of Medicine Sciences & Peking Union Medical College; Kunming, PR China.
authors.forename: Shuaiyao
authors.initials: S
authors.lastname: Lu
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases; Institute of Medical Biology; Chinese Academy of Medicine Sciences & Peking Union Medical College; Kunming, PR China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Jiangsu Convac Biotechnology Co., Ltd; Taizhou Jiangsu, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases; Institute of Medical Biology; Chinese Academy of Medicine Sciences & Peking Union Medical College; Kunming, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Liao
authors.affiliation: Jiangsu Convac Biotechnology Co., Ltd; Taizhou Jiangsu, PR China.
authors.forename: Congwen
authors.initials: C
authors.lastname: Shao
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases; Institute of Medical Biology; Chinese Academy of Medicine Sciences & Peking Union Medical College; Kunming, PR China.
authors.forename: Qihan
authors.initials: Q
authors.lastname: Li
mesh_terms: D000818:Animals; D002460:Cell Line; D004171:Diploidy; D004770:Enterovirus / Q000276:immunology*; D005260:Female; D006232:Hand, Foot and Mouth Disease / Q000276:immunology / Q000517:prevention & control; D000882:Haplorhini; D006801:Humans; D055261:Immunogenetic Phenomena / Q000187:drug effects / Q000276:immunology*; D008253:Macaca mulatta; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D015164:Vaccines, Inactivated; D014765:Viral Vaccines
keywords: coxsackie virus A 16 type; hand, foot, and mouth disease; human diploid cell line; immunogenicity; inactivated vaccine