title: Protein tagging reveals new insights into signaling in flagella.
abstract: In this issue, Oda et al. (2014. J. Cell Biol. http://dx.doi.org/10.1083/jcb.201312014) use mutant analysis, protein tagging, and cryoelectron tomography to determine the detailed location of components in flagellar radial spokes-a complex of proteins that connect the peripheral microtubule doublets to the central pair. Remarkably, this approach revealed an interaction between radial spokes and the central pair based on geometry rather than a specific signaling mechanism, highlighting the importance of studying a system in three dimensions.
authors.affiliation: Laboratory of Biomolecular Research, Paul Scherrer Institute, 5232 Villigen PSI, Switzerland.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ishikawa
mesh_terms: D056444:Axonemal Dyneins / Q000502:physiology*; D016825:Chlamydomonas reinhardtii / Q000378:metabolism*; D005407:Flagella / Q000502:physiology*
chemical_list: D056444:Axonemal Dyneins