title: Bepridil Suppresses Apoptosis in HL-1 Cardiac Atrial Myocytes Expressing Mutant E334K cMyBPC.
abstract: Besides its antiarrhythmic effect on atrial fibrillation, bepridil protects tissue, yet its effect on apoptosis has never been fully tested. We examine the effect of bepridil on apoptosis of HL-1 cells expressing E334K myosin-binding protein C (MyBPC), a model cell of apoptosis. Bepridil was compared with amiodarone, and its effects on the expression of pro- and anti-apoptotic protein and apoptosis of HL-1 cells expressing mutant E334K MyBPC-green fluorescent protein (GFP) was analyzed using Western blot and a flow cytometer. Bepridil decreased the protein levels of both Bax and cytochrome c of cells expressing E334K MyBPC-GFP with no changes in p53 and Bcl-2, while amiodarone decreased cytochrome c but did not influence Bax except in its highest concentration. It also decreased the number of Annexin-V positive cells of HL-1 cells expressing E334K MyBPC-GFP, and decreased apoptosis of HL-1 cells expressing E334K MyBPC-GFP.
authors.affiliation: Division of Anesthesiology and Critical Care Medicine, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine, Yonago 683-8504, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Endo
authors.affiliation: †Division of Regenerative Medicine and Therapeutics, Department of Genetic Medicine and Regenerative Therapeutics, Institute of Regenerative Medicine and Biofunction, Tottori University, Yonago 683-8503, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Notsu
authors.affiliation: †Division of Regenerative Medicine and Therapeutics, Department of Genetic Medicine and Regenerative Therapeutics, Institute of Regenerative Medicine and Biofunction, Tottori University, Yonago 683-8503, Japan.
authors.forename: Mutsuo
authors.initials: M
authors.lastname: Mishima
authors.affiliation: †Division of Regenerative Medicine and Therapeutics, Department of Genetic Medicine and Regenerative Therapeutics, Institute of Regenerative Medicine and Biofunction, Tottori University, Yonago 683-8503, Japan.
authors.forename: Kumi
authors.initials: K
authors.lastname: Morikawa
authors.affiliation: †Division of Regenerative Medicine and Therapeutics, Department of Genetic Medicine and Regenerative Therapeutics, Institute of Regenerative Medicine and Biofunction, Tottori University, Yonago 683-8503, Japan.
authors.forename: Peili
authors.initials: P
authors.lastname: Li
authors.affiliation: †Division of Regenerative Medicine and Therapeutics, Department of Genetic Medicine and Regenerative Therapeutics, Institute of Regenerative Medicine and Biofunction, Tottori University, Yonago 683-8503, Japan.
authors.forename: Nobuhito
authors.initials: N
authors.lastname: Ikeda
authors.affiliation: ‡Department of Biological Regulation, School of Health Science, Tottori University Faculty of Medicine, Yonago 683-8503, Japan.
authors.forename: Haruaki
authors.initials: H
authors.lastname: Ninomiya
authors.affiliation: †Division of Regenerative Medicine and Therapeutics, Department of Genetic Medicine and Regenerative Therapeutics, Institute of Regenerative Medicine and Biofunction, Tottori University, Yonago 683-8503, Japan.
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Shirayoshi
authors.affiliation: †Division of Regenerative Medicine and Therapeutics, Department of Genetic Medicine and Regenerative Therapeutics, Institute of Regenerative Medicine and Biofunction, Tottori University, Yonago 683-8503, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Hisatome
keywords: HL-1 cell; amiodarone; apoptosis; bepridil