title: Expression profile of fas-fas ligand in spiral ganglion cells during apoptosis.
abstract: OBJECTIVE
  To examine the expression profile of Fas-Fas ligand (FasL) during glutamate (Glu)-induced spiral ganglion cell (SGC) apoptosis.
  
  
  METHODS
  Cultured SGCs were treated with 10-mM, 25-mM, and 50-mM concentrations of Glu and incubated for 24 or 48 hours. The expression intensity of FasL, Fas, caspase 3, and morphology of single SGC were evaluated using immunofluorescence staining.
  
  
  RESULTS
  In semiquantitative analysis of the Glu-treated SGC, FasL, and caspase 3 expression intensity were increased with concentration- and time-dependent manner. Fas expression intensity did not change with different concentration at 48 hours. In morphologic analysis of the Glu-treated SGC, number of apoptotic cells were increased with concentration- and time-dependent manner.
  
  
  CONCLUSIONS
  FasL was expressed in apoptotic SGCs, suggesting that the Fas-FasL signaling pathway may be involved in the Glu-induced apoptosis of dissociated SGCs.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Medical Science Research Institute, Dong-A University College of Medicine, Busan, Korea.
authors.forename: Tae Woo
authors.initials: TW
authors.lastname: Gu
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Medical Science Research Institute, Dong-A University College of Medicine, Busan, Korea.
authors.forename: Woo Yong
authors.initials: WY
authors.lastname: Bae
authors.affiliation: Department of Physiology, Medical Science Research Institute, Dong-A University College of Medicine, Busan, Korea.
authors.forename: Hwan Tae
authors.initials: HT
authors.lastname: Park
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Medical Science Research Institute, Dong-A University College of Medicine, Busan, Korea.
authors.forename: Jae Hoon
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Medical Science Research Institute, Dong-A University College of Medicine, Busan, Korea.
authors.forename: Min Young
authors.initials: MY
authors.lastname: Kang
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Medical Science Research Institute, Dong-A University College of Medicine, Busan, Korea.
authors.forename: Sung Wook
authors.initials: SW
authors.lastname: Jeong
authors.affiliation: Department of Physiology, Medical Science Research Institute, Dong-A University College of Medicine, Busan, Korea.
authors.forename: Yoon Kyung
authors.initials: YK
authors.lastname: Shin
keywords: Apoptosis; Caspase 3; Fas ligand; Glutamate; Spiral ganglion cell