title: Bark essential oil of Cedrelopsis grevei from Madagascar: investigation of steam-distillation conditions.
abstract: The effect of the distillation time on the yield and chemical composition of the bark essential oil of Cedrelopsis grevei Baill. was investigated. Distillation kinetics were determined for three batches of bark sampled from two sites, i.e., Itampolo (batches IT1 and IT2) and Salary (SAL), located in a region in the south of Madagascar with characteristically large populations of C. grevei. The bark samples were subjected to steam distillation, and the essential oil was collected at 3-h intervals. The total yield (calculated after 14 h of distillation) varied from 0.9 to 1.7%, according to the batch tested. Moreover, the essential oils obtained were characterized by GC-FID and GC/MS analyses. During the course of the distillation, the relative percentages of the most volatile components (monoterpenes and sesquiterpene hydrocarbons) diminished progressively, whereas the least volatile ones (oxygenated derivatives) increased at a consistent rate. Principal component analysis (PCA) and agglomerative hierarchical clustering analysis (AHC) of the results, performed on 13 principal components, allowed distinguishing three chemical groups, corresponding to the three batches, irrespective of the distillation time. This indicated that the chemical variability currently observed with commercial samples is not mainly linked to the experimental conditions of the extraction process, as the distillation time did not significantly alter the chemical composition of the essential oils.
authors.affiliation: Département de Biologie et Ecologie végétales, Université d'Antananarivo, BP 906, Antananarivo, Madagascar; CIRAD, DP Forêts et Biodiversité, BP 853, Antananarivo, Madagascar. raktb_raouel@hotmail.fr.
authors.forename: Miarantsoa
authors.initials: M
authors.lastname: Rakotobe
authors.forename: Chantal
authors.initials: C
authors.lastname: Menut
authors.forename: Hanitriniaina Sahondra
authors.initials: HS
authors.lastname: Andrianoelisoa
authors.forename: Voninavoko
authors.initials: V
authors.lastname: Rahajanirina
authors.forename: Jean Michel Leong Pock
authors.initials: JM
authors.lastname: Tsy
authors.forename: Vonjison
authors.initials: V
authors.lastname: Rakotoarimanana
authors.forename: Perle
authors.initials: P
authors.lastname: Ramavovololona
authors.forename: Pascal
authors.initials: P
authors.lastname: Danthu
mesh_terms: D008270:Madagascar; D009822:Oils, Volatile / Q000737:chemistry* / Q000302:isolation & purification; D024301:Plant Bark / Q000737:chemistry*; D025341:Principal Component Analysis; D027821:Rutaceae / Q000737:chemistry*
chemical_list: D009822:Oils, Volatile
keywords: Cedrelopsis grevei; Essential oils; Hierarchical cluster analysis (HCA); Principal component analysis (PCA)