title: Abnormal mitosis induced by wheat-rye 1R monosomic addition lines.
abstract: Octoploid triticale were derived from common wheat (Triticum aestivum L. 'Mianyang11') × rye (Secale cereale L. 'Kustro'), and some progeny were obtained by the backcrossing of triticale with 'Mianyang11' followed by self-fertilization. In situ hybridization using rye genomic DNA and repetitive sequences pAs1 and pSc119.2 as probes was used to analyze the mitotic chromosomes of these progeny. Three wheat-rye 1R monosomic addition lines and a wheat line (12FT-1685) containing a 1R and a 1BL.1RS translocation chromosome were identified. Abnormal mitosis was observed in the two lines. During mitosis of a 1R monosomic addition line (3-8-20-1R-2), lagging chromosomes, micronuclei, chromosomal bridges, and the one pole segregation of 1R chromosome were observed. Abnormal mitotic behaviour of chromosomes was also observed in some of the self-progeny plants of lines 12FT-1685 and 3-8-20-1R-2. These progeny contained 1R chromosome or 1R chromosome arm. In addition, 4B chromosomes were absent from one of the progeny of 3-8-20-1R-2. This abnormal mitotic behaviour of chromosomes was not observed in two other 1R monosomic addition lines. These results indicate that a single 1R chromosome added to wheat might cause abnormal mitotic behaviour of both wheat and rye chromosomes and different genetic variations might occurr among the sibling 1R monosomic addition lines.
authors.affiliation: State Key Laboratory of Plant Breeding and Genetics, Sichuan Agriculture University, Wenjiang, Chengdu, Sichuan 611130, China.
authors.forename: Shu-Lan
authors.initials: SL
authors.lastname: Fu
authors.forename: Man-Yu
authors.initials: MY
authors.lastname: Yang
authors.forename: Zheng-Long
authors.initials: ZL
authors.lastname: Ren
authors.forename: Ben-Ju
authors.initials: BJ
authors.lastname: Yan
authors.forename: Zong-Xiang
authors.initials: ZX
authors.lastname: Tang
mesh_terms: D002869:Chromosome Aberrations; D032461:Chromosomes, Plant; D014644:Genetic Variation; D006824:Hybridization, Genetic; D017404:In Situ Hybridization, Fluorescence; D008938:Mitosis; D009006:Monosomy; D012434:Secale / Q000166:cytology / Q000235:genetics*; D014178:Translocation, Genetic; D014908:Triticum / Q000166:cytology / Q000235:genetics*