title: Role of IGF1 and IGF1/VEGF on human mesenchymal stromal cells in bone healing: two sources and two fates.
abstract: In the repair of skeletal defects one of the major obstacles still remains an efficient vascularization of engineered scaffolds. We have examined the ability of insulin growth factor-1, alone or in association with vascular endothelial growth factor, to modulate the osteoblastic or endothelial commitment of periosteum-derived progenitor cells (PDPCs) and skin-derived multipotent stromal cells (S-MSCs). A selected gene panel for endothelial and osteoblastic differentiation as well as genes that can affect MAPK and PI3K/AKT signaling pathways were investigated. Moreover, gene expression profile of Sox2, Oct4, and Nanog transcription factors was assessed. Our results showed that under growth factor stimulation PDPCs are induced toward an osteoblastic differentiation, while S-MSCs seem to move along an endothelial phenotype. This different commitment seems to be linked to a diverse MAPK or PI3K/AKT signaling pathway activation. The analysis of genes for stemness evidenced that at least in PDPCs multipotency and differentiation could coexist. These results open interesting perspective for the development of innovative bone tissue engineering approaches based on a good network of angiogenesis and osteogenesis processes.
authors.affiliation: 1 Department of Clinical and Molecular Sciences, Università Politecnica delle Marche , Ancona, Italy .
authors.forename: Concetta
authors.initials: C
authors.lastname: Ferretti
authors.forename: Giovanni
authors.initials: G
authors.lastname: Vozzi
authors.forename: Mirella
authors.initials: M
authors.lastname: Falconi
authors.forename: Monia
authors.initials: M
authors.lastname: Orciani
authors.forename: Marco
authors.initials: M
authors.lastname: Gesi
authors.forename: Roberto
authors.initials: R
authors.lastname: Di Primio
authors.forename: Monica
authors.initials: M
authors.lastname: Mattioli-Belmonte
mesh_terms: D000328:Adult; D001846:Bone Development / Q000187:drug effects / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000008:administration & dosage*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology*; D042461:Vascular Endothelial Growth Factor A / Q000008:administration & dosage*
chemical_list: C583557:IGF1 protein, human; D042461:Vascular Endothelial Growth Factor A; D007334:Insulin-Like Growth Factor I