title: Effects of electromagnetic fields on reelin and Dab1 expression in the developing cerebral cortex.
abstract: Many studies describe the biological effects of electromagnetic fields (EMF) including brain damages, neuronal migration and neurogenesis within the central nervous system. Neuronal cell produced in the neuroepithelium migrates along radial glial fibers into the cortical plate. Reelin, which is produced by Cajal-Retzius cells directs neuronal migration. It was shown that Disabled 1 (Dab1) functions downstream of reelin signal transduction pathway that directs the correct cytoarchitecture of the developing cortex. In this study, the EMF effects on total protein concentration (TPC), reelin and Dab1 expression in the developing cortex was studied. 30 pregnant Balb/c mice were separated into three groups: control (n = 10), EMF (n = 10) and SHAM groups (n = 10). The 15-day pregnant mice were placed inside the solenoid for a daily EMF exposure of 5 h for 3 consecutive days (15-17). The SHAM group was also located in the same coil with no exposure. Mice were sacrificed 24 h after the final exposure session. TPC, reelin and Dab1 expression were studied by Bio-Rad protein assay and western blot. No significant change in the TPC was seen in the EMF-treated cerebral cortex samples compared with those from the SHAM and control groups. It was also shown that the reelin and Dab1 expression increases in the EMF-treated cerebral cortex extracts as compared to controls and SHAM group. It is concluded that EMF may play important role in the neural cell migration by increasing reelin and Dab1 expression in the developing cortex.
authors.affiliation: Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran.
authors.forename: Matin
authors.initials: M
authors.lastname: Hemmati
authors.forename: Farhad
authors.initials: F
authors.lastname: Mashayekhi
authors.forename: Fareheh
authors.initials: F
authors.lastname: Firouzi
authors.forename: Masoumeh
authors.initials: M
authors.lastname: Ashori
authors.forename: Hamidreza
authors.initials: H
authors.lastname: Mashayekhi
mesh_terms: D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000096:biosynthesis* / Q000235:genetics; D002465:Cell Movement / Q000528:radiation effects; D002540:Cerebral Cortex / Q000196:embryology / Q000378:metabolism / Q000528:radiation effects*; D004574:Electromagnetic Fields; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D005333:Fetus / Q000528:radiation effects; D018507:Gene Expression Regulation, Developmental / Q000528:radiation effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009474:Neurons / Q000378:metabolism / Q000528:radiation effects; D011247:Pregnancy; D000091042:Reelin Protein; D012697:Serine Endopeptidases / Q000096:biosynthesis* / Q000235:genetics; D016037:Single-Blind Method
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; C103773:Dab1 protein, mouse; D016326:Extracellular Matrix Proteins; D009419:Nerve Tissue Proteins; D000091042:Reelin Protein; C000717128:Reln protein, mouse; D012697:Serine Endopeptidases