title: Development of a vectored vaccine against hepatitis E virus.
abstract: Hepatitis E virus is a non-enveloped ssRNA virus [1] that causes human acute hepatitis through primarily fecal and oral transmission [2]. Currently, no commercial hepatitis E (HEV) vaccine is available. In the absence of an appropriate cell culture system for HEV propagation, HEV pseudocapsids (ORF2 protein) have been produced either in Escherichia coli or in insect cells and they have been shown to protect monkeys against virus challenge and to be effective in the prevention of natural HEV infection of humans. In this work, we propose to develop a novel candidate vaccine against hepatitis E infection using adeno-associated virus (AAV) as a vector expressing the gene of the truncated capsid protein of HEV (aa 112-aa 660). rAAV will be produced in Sf9 cells using the baculovirus expression vector system. For this purpose, construction of recombinant baculoviruses was performed and viral stocks of BacRep, BacCap for serotypes 2, 5 and 6 were prepared in Sf9 cells. The recombinant baculovirus coding for the truncated capsid protein of HEV (BacITRHEVORF2) was also constructed, the virus titer was equal to 5.41×10(9) PFU/mL, at the third passage. Transduction of HEK 293 EBNA cells with rAAV was carried out; the production of HEVORF2 was confirmed by Western blot. Optimization of rAAV production in Sf9 cells is currently ongoing.
authors.affiliation: Viral Vaccines Research & Development Unit, Laboratory of Molecular Microbiology, Vaccinology and Biotechnology Development, Institut Pasteur de Tunis, 13 Place Pasteur, BP 74 1002 Tunis, Tunisia.
authors.forename: Khaled
authors.initials: K
authors.lastname: Trabelsi
authors.affiliation: Animal Cell Technology, Biotechnology Research Institute, National Research Council of Canada, Montreal, Canada.
authors.forename: Amine
authors.initials: A
authors.lastname: Kamen
authors.affiliation: Viral Vaccines Research & Development Unit, Laboratory of Molecular Microbiology, Vaccinology and Biotechnology Development, Institut Pasteur de Tunis, 13 Place Pasteur, BP 74 1002 Tunis, Tunisia. Electronic address: hela.kallel@pasteur.rns.tn.
authors.forename: Héla
authors.initials: H
authors.lastname: Kallel
mesh_terms: D000818:Animals; D000229:Dependovirus / Q000276:immunology; D005822:Genetic Vectors / Q000276:immunology*; D057809:HEK293 Cells; D016752:Hepatitis E virus; D006801:Humans; D019590:Nucleocapsid Proteins / Q000276:immunology*; D061987:Sf9 Cells; D018411:Spodoptera; D014161:Transduction, Genetic; D014761:Viral Hepatitis Vaccines / Q000276:immunology*
chemical_list: D019590:Nucleocapsid Proteins; D014761:Viral Hepatitis Vaccines; C099600:nucleocapsid protein, Hepatitis virus
keywords: Adeno-associated virus; Baculovirus; Hepatitis E virus; Insect cells SF9