title: Dendritic cells: In vitro culture in two- and three-dimensional collagen systems and expression of collagen receptors in tumors and atherosclerotic microenvironments.
abstract: Dendritic cells (DCs) are immune cells found in the peripheral tissues where they sample the organism for infections or malignancies. There they take up antigens and migrate towards immunological organs to contact and activate T lymphocytes that specifically recognize the antigen presented by these antigen presenting cells. In the steady state there are several types of resident DCs present in various different organs. For example, in the mouse, splenic DC populations characterized by the co-expression of CD11c and CD8 surface markers are specialized in cross-presentation to CD8 T cells, while CD11c/SIRP-1α DCs seem to be dedicated to activating CD4 T cells. On the other hand, DCs have also been associated with the development of various diseases such as cancer, atherosclerosis, or inflammatory conditions. In such disease, DCs can participate by inducing angiogenesis or immunosuppression (tumors), promoting autoimmune responses, or exacerbating inflammation (atherosclerosis). This change in DC biology can be prompted by signals in the microenvironment. We have previously shown that the interaction of DCs with various extracellular matrix components modifies the immune properties and angiogenic potential of these cells. Building on those studies, herewith we analyzed the angiogenic profile of murine myeloid DCs upon interaction with 2D and 3D type-I collagen environments. As determined by PCR array technology and quantitative PCR analysis we observed that interaction with these collagen environments induced the expression of particular angiogenic molecules. In addition, DCs cultured on collagen environments specifically upregulated the expression of CXCL-1 and -2 chemokines. We were also able to establish DC cultures on type-IV collagen environments, a collagen type expressed in pathological conditions such as atherosclerosis. When we examined DC populations in atherosclerotic veins of Apolipoprotein E deficient mice we observed that they expressed adhesion molecules capable of interacting with collagen. Finally, to further investigate the interaction of DCs with collagen in other pathological conditions, we determined that both murine ovarian and breast cancer cells express several collagen molecules that can contribute to shape their particular tumor microenvironment. Consistently, tumor-associated DCs were shown to express adhesion molecules capable of interacting with collagen molecules as determined by flow cytometry analysis. Of particular relevance, tumor-associated DCs expressed high levels of CD305/LAIR-1, an immunosuppressive receptor. This suggests that signaling through this molecule upon interaction with collagen produced by tumor cells might help define the poorly immunogenic status of these cells in the tumor microenvironment. Overall, these studies demonstrate that through interaction with collagen proteins, DCs can be capable of modifying the microenvironments of inflammatory disease such as cancer or atherosclerosis.
authors.affiliation: Biomedical Engineering Program, Russ College of Engineering and Technology, Ohio University, USA.
authors.forename: Leslee
authors.initials: L
authors.lastname: Sprague
authors.affiliation: Molecular and Cellular Biology Program, Ohio University, USA.
authors.forename: Maria
authors.initials: M
authors.lastname: Muccioli
authors.affiliation: Department of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University, USA.
authors.forename: Michelle
authors.initials: M
authors.lastname: Pate
authors.affiliation: Molecular and Cellular Biology Program, Ohio University, USA.
authors.forename: Manindra
authors.initials: M
authors.lastname: Singh
authors.affiliation: Biomedical Engineering Program, Russ College of Engineering and Technology, Ohio University, USA.
authors.forename: Chengkai
authors.initials: C
authors.lastname: Xiong
authors.affiliation: Department of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University, USA.
authors.forename: Alexander
authors.initials: A
authors.lastname: Ostermann
authors.affiliation: Department of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University, USA.
authors.forename: Brandon
authors.initials: B
authors.lastname: Niese
authors.affiliation: Molecular and Cellular Biology Program, Ohio University, USA.
authors.forename: Yihan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Molecular and Cellular Biology Program, Ohio University, USA.
authors.forename: Yandi
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University, USA.
authors.forename: Maria Cecilia
authors.initials: MC
authors.lastname: Courreges
authors.affiliation: Biomedical Engineering Program, Russ College of Engineering and Technology, Ohio University, USA; Molecular and Cellular Biology Program, Ohio University, USA; Diabetes Institute, Ohio University, USA; Department of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University, USA. Electronic address: benencia@ohio.edu.
authors.forename: Fabian
authors.initials: F
authors.lastname: Benencia
mesh_terms: D000818:Animals; D001057:Apolipoproteins E / Q000235:genetics; D050197:Atherosclerosis / Q000276:immunology / Q000378:metabolism*; D001943:Breast Neoplasms / Q000276:immunology / Q000378:metabolism*; D039521:CD11c Antigen / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D054360:Chemokine CXCL1 / Q000096:biosynthesis; D054426:Chemokine CXCL2 / Q000096:biosynthesis; D002633:Chemotaxis; D003094:Collagen / Q000378:metabolism; D003713:Dendritic Cells / Q000378:metabolism*; D005260:Female; D039222:Integrin alpha1beta1 / Q000096:biosynthesis / Q000378:metabolism; D038982:Integrin alpha2beta1 / Q000096:biosynthesis / Q000378:metabolism; D039201:Integrin alpha3beta1 / Q000096:biosynthesis / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009374:Neoplasms, Experimental / Q000276:immunology / Q000378:metabolism; D018919:Neovascularization, Physiologic; D010051:Ovarian Neoplasms / Q000276:immunology / Q000378:metabolism*; D038981:Receptors, Collagen / Q000096:biosynthesis / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000378:metabolism; D051117:Scavenger Receptors, Class A / Q000096:biosynthesis / Q000378:metabolism; D059016:Tumor Microenvironment; D015854:Up-Regulation
chemical_list: D001057:Apolipoproteins E; D039521:CD11c Antigen; D015815:Cell Adhesion Molecules; D054360:Chemokine CXCL1; D054426:Chemokine CXCL2; C475895:Cxcl1 protein, mouse; C404915:Cxcl2 protein, mouse; D039222:Integrin alpha1beta1; D038982:Integrin alpha2beta1; D039201:Integrin alpha3beta1; C495140:Msr1 protein, mouse; D038981:Receptors, Collagen; D011971:Receptors, Immunologic; D051117:Scavenger Receptors, Class A; C108073:leukocyte-associated immunoglobulin-like receptor 1; D003094:Collagen
keywords: Angiogenesis; Antigen presentation; Atherosclerosis; Breast cancer; Collagen; Dendritic cells; Ovarian cancer