title: Analysis of RNA-protein interactions by cell mixing.
abstract: RNA-protein complexes are critical for almost all aspects of gene expression. Analysis of RNA-protein interactions can be complicated by the disruption of native complexes and the formation of new, reassorted complexes upon cell lysis. Before concluding that a specific RNA and protein interact in vivo, cell-mixing experiments can be performed to ensure that observed RNA-protein complexes are not formed after lysis of cells.
authors.affiliation: Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
authors.forename: Sarah H
authors.initials: SH
authors.lastname: Stubbs
authors.affiliation: Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX, USA. Electronic address: nicholas.conrad@utsouthwestern.edu.
authors.forename: Nicholas K
authors.initials: NK
authors.lastname: Conrad
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002457:Cell Extracts / Q000737:chemistry; D002458:Cell Fractionation; D002474:Cell-Free System; D002478:Cells, Cultured; D006801:Humans; D047468:Immunoprecipitation; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D017411:Ribonucleoproteins, Small Nuclear / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002457:Cell Extracts; D017411:Ribonucleoproteins, Small Nuclear; D012313:RNA
keywords: Cell lysis; Cell mixing; Co-immunoprecipitation (co-IP) assays; Immunoprecipitation; Northern and Western blotting; Proteinase K digestion; RNA–protein interactions; Small nucleolar ribonucleoprotein particle (snoRNP)