title: Bradyrhizobium ganzhouense sp. nov., an effective symbiotic bacterium isolated from Acacia melanoxylon R. Br. nodules.
abstract: Three slow-growing rhizobial strains, designated RITF806(T), RITF807 and RITF211, isolated from root nodules of Acacia melanoxylon grown in Ganzhou city, Jiangxi Province, China, had been previously defined, based on amplified 16S rRNA gene restriction analysis, as a novel group within the genus Bradyrhizobium. To clarify their taxonomic position, these strains were further analysed and compared with reference strains of related bacteria using a polyphasic approach. According to 16S rRNA gene sequence analysis, the isolates formed a group that was closely related to 'Bradyrhizobium rifense' CTAW71, with a similarity value of 99.9%. In phylogenetic analyses of the housekeeping and symbiotic gene sequences, the three strains formed a distinct lineage within the genus Bradyrhizobium, which was consistent with the results of DNA-DNA hybridization. In analyses of cellular fatty acids and phenotypic features, some differences were found between the novel group and related species of the genus Bradyrhizobium, indicating that these three strains constituted a novel group distinct from any recognized species of the genus Bradyrhizobium. Based on the data obtained in this study, we conclude that our strains represent a novel species of the genus Bradyrhizobium, for which the name Bradyrhizobium ganzhouense sp. nov. is proposed, with RITF806(T) ( = CCBAU 101088(T) = JCM 19881(T)) as the type strain. The DNA G+C content of strain RITF806(T) is 64.6 mol% (T(m)).
authors.affiliation: Research Institute of Tropical Forestry, Guangzhou 510520, PR China.
authors.forename: Jun Kun
authors.initials: JK
authors.lastname: Lu
authors.affiliation: Research Institute of Tropical Forestry, Guangzhou 510520, PR China.
authors.forename: Ya Jing
authors.initials: YJ
authors.lastname: Dou
authors.affiliation: Research Institute of Tropical Forestry, Guangzhou 510520, PR China.
authors.forename: Ya Jie
authors.initials: YJ
authors.lastname: Zhu
authors.affiliation: Research Institute of Tropical Forestry, Guangzhou 510520, PR China.
authors.forename: Sheng Kun
authors.initials: SK
authors.lastname: Wang
authors.affiliation: State Key Laboratories for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Xin Hua
authors.initials: XH
authors.lastname: Sui
authors.affiliation: Research Institute of Tropical Forestry, Guangzhou 510520, PR China.
authors.forename: Li Hua
authors.initials: LH
authors.lastname: Kang
mesh_terms: D000045:Acacia / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D020369:Bradyrhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D053204:Root Nodules, Plant / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013559:Symbiosis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S