title: Metabolomic profiling of mice urine and serum associated with trans-trans 2, 4-decadienal induced lung lesions by liquid chromatography-mass spectrometry.
abstract: Metabolomics has become an important tool in clinical research and the diagnosis of human disease. Intratracheal instillation of trans-trans 2,4-decadienal (tt-DDE), a major component in cooking oil fumes, has been demonstrated to cause lung lesions in mice at 8 weeks after treatment. The objective of this study was to identify any changes in metabolite profiles associated with the development of tt-DDE-induced lung lesions. Using a metabolomics strategy involving a liquid chromatography-mass spectrometry-based approach in conjunction with principal component analysis and confirmation by liquid chromatography triple quadrupole tandem mass spectrometry, we have demonstrated that the amino acid profiles of the urine and serum of tt-DDE-treated mice are changed. Ten amino acids were significantly reduced in serum of tt-DDE-treated mice at 8 weeks after treatment. Our results suggest that amino acid profiles may be useful as an early indicator of the presence of tt-DDE-induced lung lesions.
authors.affiliation: Division of Environmental Health and Occupational Medicine, National Health Research Institutes, Zhunan, Miaoli County, 350, Taiwan, Republic of China.
authors.forename: Pinpin
authors.initials: P
authors.lastname: Lin
authors.forename: Hui-Ling
authors.initials: HL
authors.lastname: Lee
authors.forename: Hao-I
authors.initials: HI
authors.lastname: Cheng
authors.forename: Chao-Yu
authors.initials: CY
authors.lastname: Chen
authors.forename: Ming-Hsien
authors.initials: MH
authors.lastname: Tsai
authors.forename: Huei-Ju
authors.initials: HJ
authors.lastname: Liu
mesh_terms: D000447:Aldehydes / Q000097:blood* / Q000633:toxicity / Q000652:urine*; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D008171:Lung Diseases / Q000097:blood / Q000209:etiology* / Q000652:urine; D008297:Male; D013058:Mass Spectrometry; D055432:Metabolomics / Q000379:methods*; D051379:Mice; D008813:Mice, Inbred ICR
chemical_list: D000447:Aldehydes; C057349:2,4-decadienal