title: Characterization of QmnD3/QmnD4 for double bond formation in quartromicin biosynthesis.
abstract: In this work, two enzymes responsible for the biogenesis of possible [4 + 2] reaction precursors in the quartromicin biosynthetic pathway were characterized: acetylation of 1 to yield 2 was catalyzed by QmnD3, and subsequent acetic acid elimination of 2 to form double bond product 3 was catalyzed by QmnD4. Site-directed mutagenesis assay of QmnD3 and QmnD4 was investigated, and a general base-catalyzed mechanism for QmnD4 is proposed.
authors.affiliation: State Key Laboratory of Bio-organic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 345 Lingling Road, Shanghai 200032, China.
authors.forename: Long-Fei
authors.initials: LF
authors.lastname: Wu
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: He
authors.forename: Hai-Xue
authors.initials: HX
authors.lastname: Pan
authors.forename: Li
authors.initials: L
authors.lastname: Han
authors.forename: Renxiao
authors.initials: R
authors.lastname: Wang
authors.forename: Gong-Li
authors.initials: GL
authors.lastname: Tang
mesh_terms: D002384:Catalysis; D005656:Fungal Proteins / Q000378:metabolism; D005663:Furans / Q000138:chemical synthesis* / Q000737:chemistry; D006867:Hydrolases / Q000378:metabolism*; D008049:Lipase / Q000378:metabolism; D015394:Molecular Structure; D013141:Spiro Compounds / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D005656:Fungal Proteins; D005663:Furans; D013141:Spiro Compounds; C457021:quartromicin; D006867:Hydrolases; D008049:Lipase; C431349:lipase B, Candida antarctica