title: The role of SRC1 and SRC2 in steroid-induced SDF1 expression in normal and ectopic endometrium.
abstract: To compare the expression patterns of steroid receptor coactivators (SRCs) and steroid-induced stromal cell-derived factor 1 (CXCL12 (SDF1)) in normal and ectopic endometrium and to explore the roles of NCOA1 (SRC1) and NCOA2 (SRC2) in the steroid-induced CXCL12 expression in normal and ectopic endometrial stromal cells (ESCs). The NCOA1, NCOA2, NCOA3 (SRC3), and CXCL12 (SDF1)α mRNA levels in normal and ectopic endometrium were analyzed by quantitative real-time PCR. Steroid-induced CXCL12 expression was detected by the ELISA method and the chemotactic activity of conditioned supernatant to monocyte was assessed by the Boyden chamber method before and after the silencing of NCOA1 or NCOA2 with siRNA in normal and ectopic ESCs. The expression of NCOA1 and CXCL12 in ectopic endometrium was significantly greater than that in normal endometrium in the secretory phase. Progesterone (P4) was able to significantly inhibit estradiol (E2)-stimulated CXCL12 expression in normal and ectopic ESCs. The inhibitory rate of P4 in ectopic ESCs at 72 and 96 h was significantly lower than that in normal ESCs. Silencing of NCOA1 but not NCOA2 significantly reduced the E2-induced CXCL12 expression in normal and ectopic ESCs. The ability of P4 to inhibit E2-induced CXCL12 expression and monocyte chemotaxis in normal and ectopic ESCs was significantly attenuated when NCOA2 was silenced. NCOA1 plays a necessary role in E2-induced CXCL12 expression and NCOA2 is required for P4 to inhibit the E2-induced CXCL12 production in normal and ectopic endometrium.
authors.affiliation: The Department of GynecologyThe First Affiliated Hospital of Nanjing Medical University (also named Jiangsu Province Hospital), No. 368 North-Jiangdong Road, Gulou, Nanjing, Jiangsu 210036, People's Republic of China.
authors.forename: Xiu
authors.initials: X
authors.lastname: Shi
authors.affiliation: The Department of GynecologyThe First Affiliated Hospital of Nanjing Medical University (also named Jiangsu Province Hospital), No. 368 North-Jiangdong Road, Gulou, Nanjing, Jiangsu 210036, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.affiliation: The Department of GynecologyThe First Affiliated Hospital of Nanjing Medical University (also named Jiangsu Province Hospital), No. 368 North-Jiangdong Road, Gulou, Nanjing, Jiangsu 210036, People's Republic of China.
authors.forename: Hui-Hua
authors.initials: HH
authors.lastname: Dai
authors.affiliation: The Department of GynecologyThe First Affiliated Hospital of Nanjing Medical University (also named Jiangsu Province Hospital), No. 368 North-Jiangdong Road, Gulou, Nanjing, Jiangsu 210036, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.affiliation: The Department of GynecologyThe First Affiliated Hospital of Nanjing Medical University (also named Jiangsu Province Hospital), No. 368 North-Jiangdong Road, Gulou, Nanjing, Jiangsu 210036, People's Republic of China xiuli_2266@163.com.
authors.forename: Xiu-Li
authors.initials: XL
authors.lastname: Wang
mesh_terms: D016022:Case-Control Studies; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000235:genetics / Q000378:metabolism*; D002634:Chemotaxis, Leukocyte; D017077:Culture Media, Conditioned / Q000378:metabolism; D004715:Endometriosis / Q000235:genetics / Q000378:metabolism*; D004717:Endometrium / Q000187:drug effects* / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D009000:Monocytes / Q000378:metabolism; D056920:Nuclear Receptor Coactivator 1 / Q000235:genetics / Q000378:metabolism*; D051958:Nuclear Receptor Coactivator 2 / Q000235:genetics / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology; D034622:RNA Interference; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014162:Transfection; D015854:Up-Regulation
chemical_list: C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D017077:Culture Media, Conditioned; C498001:NCOA2 protein, human; D051958:Nuclear Receptor Coactivator 2; D011374:Progesterone; D004958:Estradiol; C538859:NCOA1 protein, human; D056920:Nuclear Receptor Coactivator 1