title: Small molecule recognition of poly(A).
abstract: The binding of small molecules to non-canonical nucleic acid structures has been a major focus of rational drug design. Among the non-canonical nucleic acid structures, targeting poly(A) using small molecules has attracted a special interest due to the cellular functions of poly(A) tails. Here, the methods for determining the binding of a small molecule to poly(A) using UV-visible(UV-Vis) and Circular Dichroism (CD) Spectroscopy are described. Experiments used in determining the melting temperature, binding stoichiometry and dissociation constant of poly(A)-small molecule systems are depicted.
authors.affiliation: Zentiva Health Products Co., Lüleburgaz, Kırklareli, Turkey.
authors.forename: Ozgül Persil
authors.initials: OP
authors.lastname: Cetinkol
mesh_terms: D002942:Circular Dichroism; D009690:Nucleic Acid Conformation; D011061:Poly A / Q000378:metabolism*
chemical_list: D011061:Poly A