title: Impact of targeted specific antibiotic delivery for gut microbiota modulation on high-fructose-fed rats.
abstract: The objective of present investigation was to study the effect of gut microbiota alteration by oral administration of targeted delivery of pH sensitive cefdinir microspheres to high-fructose-fed (HFD) rats. Rats were fed with a high-fructose diet with or without cefdinir microsphere administration for 30 days. The fecal microbiota community, oral glucose tolerance, the markers of liver injury, plasma and hepatic lipids profile, and histological evaluation were investigated. The levels of blood glucose, liver injury markers, lipid profile in plasma and liver, and fat tissue were significantly increased in high-fructose-fed rats. However, after pH-sensitive cefdinir microsphere administration, the elevation of these parameters was significantly suppressed. Cef EL significantly lowered the increased AST (p < 0.05) and ALT (p < 0.001) levels in HFD group. There is a significant lower (p < 0.01) AUCglucose level in Cef EL group than HFD group The histological changes in the liver and the small and large intestines were more profound in HFD group as compared to cefdinir-treated HFD and control groups. Feeding of cefdinir microsphere sustained lactobacilli and bifidobacteria and significantly decreased (p < 0.05) the number of Enterobacteriaceae induced by HFD. Experimental evidences demonstrated that the effectiveness of pH-specific cefdinir microsphere on reducing insulin resistance and development of metabolic changes in high-fructose-fed rats and suggested that it may be a promising therapeutic agent in treating type 2 diabetes. Intestinal-targeted antibiotic delivery needs to be further explored for its therapeutic applications.
authors.affiliation: Institute of Science, Nirma University, Sarkhej-Gandhinagar Highway, Chandlodia, Ahmedabad, 382481, Gujarat, India.
authors.forename: Prasant Kumar
authors.initials: PK
authors.lastname: Jena
authors.forename: Shilpa
authors.initials: S
authors.lastname: Singh
authors.forename: Bhumika
authors.initials: B
authors.lastname: Prajapati
authors.forename: G
authors.initials: G
authors.lastname: Nareshkumar
authors.forename: Tejal
authors.initials: T
authors.lastname: Mehta
authors.forename: Sriram
authors.initials: S
authors.lastname: Seshadri
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology; D001774:Blood Chemical Analysis; D001835:Body Weight / Q000187:drug effects; D000077525:Cefdinir; D002511:Cephalosporins / Q000378:metabolism* / Q000494:pharmacology*; D004040:Dietary Carbohydrates / Q000009:adverse effects*; D004326:Drinking / Q000187:drug effects; D065546:Drug Liberation; D005243:Feces / Q000382:microbiology; D005632:Fructose / Q000009:adverse effects*; D005951:Glucose Tolerance Test; D007422:Intestines / Q000166:cytology / Q000187:drug effects* / Q000382:microbiology*; D008099:Liver / Q000166:cytology / Q000187:drug effects; D008297:Male; D064307:Microbiota / Q000187:drug effects*; D008863:Microspheres; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D004040:Dietary Carbohydrates; D005632:Fructose; D000077525:Cefdinir