title: Dynamic autofluorescence imaging of intracellular components inside living cells using direct electron beam excitation.
abstract: We developed a high-resolution fluorescence microscope in which fluorescent materials are directly excited using a focused electron beam. Electron beam excitation enables detailed observations on the nanometer scale. Real-time live-cell observation is also possible using a thin film to separate the environment under study from the vacuum region required for electron beam propagation. In this study, we demonstrated observation of cellular components by autofluorescence excited with a focused electron beam and performed dynamic observations of intracellular granules. Since autofluorescence is associated with endogenous substances in cells, this microscope can also be used to investigate the intrinsic properties of organelles.
authors.affiliation: Graduate School of Science and Technology, Shizuoka University, Johoku, Naka, Hamamatsu 4328561, Japan ; Research Fellow of the Japan Society for the Promotion of Science, Chiyoda, Tokyo 102-0083, Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Nawa
authors.affiliation: Faculty of Engineering, Shizuoka University, Johoku, Naka, Hamamatsu 4328561, Japan ; CREST, Japan Science and Technology Agency, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Inami
authors.affiliation: Faculty of Engineering, Shizuoka University, Johoku, Naka, Hamamatsu 4328561, Japan ; CREST, Japan Science and Technology Agency, Japan.
authors.forename: Aki
authors.initials: A
authors.lastname: Miyake
authors.affiliation: CREST, Japan Science and Technology Agency, Japan ; Research Institute of Electronics, Shizuoka University, Johoku, Naka, Hamamatsu 4328011, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ono
authors.affiliation: Graduate School of Science and Technology, Shizuoka University, Johoku, Naka, Hamamatsu 4328561, Japan ; Faculty of Engineering, Shizuoka University, Johoku, Naka, Hamamatsu 4328561, Japan ; CREST, Japan Science and Technology Agency, Japan ; Research Institute of Electronics, Shizuoka University, Johoku, Naka, Hamamatsu 4328011, Japan.
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Kawata
authors.affiliation: Faculty of Engineering, Shizuoka University, Johoku, Naka, Hamamatsu 4328561, Japan.
authors.forename: Sheng
authors.initials: S
authors.lastname: Lin
authors.affiliation: CREST, Japan Science and Technology Agency, Japan ; Photon Medical Research Center, Hamamatsu University School of Medicine, Handayama, Higashi, Hamamatsu 4313192, Japan.
authors.forename: Susumu
authors.initials: S
authors.lastname: Terakawa
keywords: (110.0180) Microscopy; (170.0180) Microscopy; (170.2520) Fluorescence microscopy; (180.2520) Fluorescence microscopy; (180.5810) Scanning microscopy