title: Fission of SNX-BAR-coated endosomal retrograde transport carriers is promoted by the dynamin-related protein Vps1.
abstract: Retromer is an endosomal sorting device that orchestrates capture and packaging of cargo into transport carriers coated with sorting nexin BAR domain proteins (SNX-BARs). We report that fission of retromer SNX-BAR-coated tubules from yeast endosomes is promoted by Vps1, a dynamin-related protein that localizes to endosomes decorated by retromer SNX-BARs and Mvp1, a SNX-BAR that is homologous to human SNX8. Mvp1 exhibits potent membrane remodeling activity in vitro, and it promotes association of Vps1 with the endosome in vivo. Retrograde transport carriers bud from the endosome coated by retromer and Mvp1, and cargo export is deficient in mvp1- and vps1-null cells, but with distinct endpoints; cargo export is delayed in mvp1-null cells, but cargo export completely fails in vps1-null cells. The results indicate that Mvp1 promotes Vps1-mediated fission of retromer- and Mvp1-coated tubules that bud from the endosome, revealing a functional link between the endosomal sorting and fission machineries to produce retrograde transport carriers.
authors.affiliation: Department of Cell Biology, Yale School of Medicine, New Haven, CT 06520.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Chi
authors.forename: Jingxuan
authors.initials: J
authors.lastname: Liu
authors.forename: Matthew
authors.initials: M
authors.lastname: West
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Greg
authors.initials: G
authors.lastname: Odorizzi
authors.forename: Christopher G
authors.initials: CG
authors.lastname: Burd
mesh_terms: D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D055032:Electron Microscope Tomography; D011992:Endosomes / Q000502:physiology* / Q000648:ultrastructure; D019204:GTP-Binding Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D008954:Models, Biological; D012097:Repressor Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000378:metabolism / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D058305:Sorting Nexins / Q000378:metabolism / Q000502:physiology; D033921:Vesicular Transport Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*
chemical_list: C092120:MVP1 protein, S cerevisiae; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D058305:Sorting Nexins; D033921:Vesicular Transport Proteins; D019204:GTP-Binding Proteins; C064553:VPS1 protein, S cerevisiae