title: Systematic analysis of a xenograft mice model for KSHV+ primary effusion lymphoma (PEL).
abstract: Kaposi's sarcoma-associated herpesvirus is the causative agent of primary effusion lymphoma (PEL), which arises preferentially in the setting of infection with human immunodeficiency virus (HIV). Even with standard cytotoxic chemotherapy, PEL continues to cause high mortality rates, requiring the development of novel therapeutic strategies. PEL xenograft models employing immunodeficient mice have been used to study the in vivo effects of a variety of therapeutic approaches. However, it remains unclear whether these xenograft models entirely reflect clinical presentations of KSHV(+) PEL, especially given the recent description of extracavitary solid tumor variants arising in patients. In addition, effusion and solid tumor cells propagated in vivo exhibit unique biology, differing from one another or from their parental cell lines propagated through in vitro culture. Therefore, we used a KSHV(+) PEL/BCBL-1 xenograft model involving non-obese diabetic/severe-combined immunodeficient (NOD/SCID) mice, and compared characteristics of effusion and solid tumors with their parent cell culture-derived counterparts. Our results indicate that although this xenograft model can be used for study of effusion and solid lymphoma observed in patients, tumor cells in vivo display unique features to those passed in vitro, including viral lytic gene expression profile, rate of solid tumor development, the host proteins and the complex of tumor microenvironment. These items should be carefully considered when the xenograft model is used for testing novel therapeutic strategies against KSHV-related lymphoma.
authors.affiliation: Research Center for Translational Medicine and Key Laboratory of Arrhythmias of the Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, China ; Department of Medicine, Louisiana Cancer Research Center, Louisiana State University Health Sciences Center, New Orleans, Louisiana, United States of America.
authors.forename: Lu
authors.initials: L
authors.lastname: Dai
authors.affiliation: Department of Pathology, Louisiana Cancer Research Center, Louisiana State University Health Sciences Center, New Orleans, Louisiana, United States of America.
authors.forename: Jimena
authors.initials: J
authors.lastname: Trillo-Tinoco
authors.affiliation: Central Laboratory, East Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Bai
authors.affiliation: Central Laboratory, East Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Baoli
authors.initials: B
authors.lastname: Kang
authors.affiliation: Central Laboratory, East Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Zengguang
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Urology, East Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Wen
authors.affiliation: Department of Pathology, Louisiana Cancer Research Center, Louisiana State University Health Sciences Center, New Orleans, Louisiana, United States of America.
authors.forename: Luis
authors.initials: L
authors.lastname: Del Valle
authors.affiliation: Research Center for Translational Medicine and Key Laboratory of Arrhythmias of the Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, China ; Departments of Microbiology, Immunology & Parasitology, Louisiana Cancer Research Center, Louisiana State University Health Sciences Center, New Orleans, Louisiana, United States of America.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Qin
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D015967:Gene Expression Regulation, Viral; D019288:Herpesvirus 8, Human / Q000235:genetics* / Q000472:pathogenicity; D054884:Host-Pathogen Interactions; D006801:Humans; D054685:Lymphoma, Primary Effusion / Q000150:complications / Q000473:pathology* / Q000821:virology; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D012514:Sarcoma, Kaposi / Q000150:complications / Q000473:pathology* / Q000821:virology; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured; D059016:Tumor Microenvironment; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D014764:Viral Proteins