title: [Interleukin-32 expression in serum of patients with HBV-related liver failure and its significance].
abstract: OBJECTIVE
  To investigate the expression level of IL-32 in serum and its correlation with serum biochemical indices of liver function test and HBV DNA load in patients with HBV-related liver failure.
  
  
  METHODS
  Fifty-five patients with HBV-related liver failure (severe hepatitis group) and twenty normal cases (control group) were enrolled in the study. Total RNA in PBMCs was extracted by using TRIzol. IL-32 mRNA level was assayed by using Real-time PCR. IL-32 protein level in serum was detected by ELSIA method. The correlation between IL-32 and ALT, AST, TBIL, HBV DNA load was analyzed using pearson's correlation analysis, respectively.
  
  
  RESULTS
  Serum IL-32 expression level in severe hepatitis group was higher than that of control group. Moreover, the difference between them was statistically significant (P < 0.05). Serum IL-32 level was positively correlated with serum ALT, AST, TBIL, respectively (P < 0.05), but was not correlated with HBV DNA load (P > 0.05).
  
  
  CONCLUSIONS
  Serum IL-32 expression level was increased in patients with HBV-related liver failure and was associated with the severity of inflammation. We, therefore, believe that IL-32 might be involved in the pathogenesis of HBV-related liver failure.
authors.affiliation: Department of Infectious Diseases, Central Hospital of Longgang, Shenzhen 518116, China.
authors.forename: Gui-Long
authors.initials: GL
authors.lastname: Zhuang
authors.affiliation: Department of Infectious Diseases, Central Hospital of Longgang, Shenzhen 518116, China.
authors.forename: Guo-Hang
authors.initials: GH
authors.lastname: Li
authors.affiliation: Department of Infectious Diseases, Central Hospital of Longgang, Shenzhen 518116, China.
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Qu
authors.affiliation: Department of Infectious Diseases, Central Hospital of Longgang, Shenzhen 518116, China.
authors.forename: Jian-Yu
authors.initials: JY
authors.lastname: Kuang
mesh_terms: D000328:Adult; D000410:Alanine Transaminase / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood; D005260:Female; D006515:Hepatitis B virus / Q000302:isolation & purification / Q000502:physiology; D019694:Hepatitis B, Chronic / Q000097:blood* / Q000201:enzymology / Q000235:genetics / Q000821:virology; D006801:Humans; D007378:Interleukins / Q000097:blood* / Q000235:genetics; D017093:Liver Failure / Q000097:blood* / Q000201:enzymology / Q000235:genetics / Q000821:virology; D008297:Male; D008875:Middle Aged; D019562:Viral Load; D055815:Young Adult
chemical_list: C496971:IL32 protein, human; D007378:Interleukins; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase