title: Highly sensitive and rapid bacteria detection using molecular beacon-Au nanoparticles hybrid nanoprobes.
abstract: Since many diseases are caused by pathogenic bacterial infections, accurate and rapid detection of pathogenic bacteria is in urgent need to timely apply appropriate treatments and to reduce economic costs. To end this, we designed molecular beacon-Au nanoparticle hybrid nanoprobes to improve the bacterial detection efficiency and sensitivity. Here, we show that the designed molecular beacon modified Au nanoparticles could specifically recognize synthetic DNAs targets and can readily detect targets in clinical samples. Moreover, the hybrid nanoprobes can recognize Escherichia coli within an hour at a concentration of 10(2) cfu/ml, which is 1000-folds sensitive than using molecular beacon directly. Our results show that the molecular beacon-Au nanoparticle hybrid nanoprobes have great potential in medical and biological applications.
authors.affiliation: Single Molecule Nanometry Laboratory, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China. Electronic address: http://www.feiliugroup.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Cao
authors.affiliation: Department of Pathology, The First People׳s Hospital of Shangqiu, Henan, Shangqiu 476000, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Feng
authors.affiliation: Clinical Laboratory, The First People׳s Hospital of Shangqiu, Henan, Shangqiu 476000, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Information Physics and Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu 210094, China.
authors.forename: Shouyu
authors.initials: S
authors.lastname: Wang
authors.affiliation: Single Molecule Nanometry Laboratory, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China. Electronic address: feiliu24@njau.edu.cn.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
mesh_terms: D015374:Biosensing Techniques / Q000191:economics / Q000379:methods*; D015342:DNA Probes / Q000737:chemistry*; D004926:Escherichia coli / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000175:diagnosis / Q000652:urine*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D012680:Sensitivity and Specificity
chemical_list: D015342:DNA Probes; D006046:Gold
keywords: Au nanoparticles; Molecular beacon; Sensitive and rapid bacterial detection