title: Cloning and analysis of a functional promoter of fungal immunomodulatory protein from Flammulina velutipes.
abstract: Fugal immunomodulatory protein from Flammulina velutipes (FIP-fve) belongs to FIPs family, which has precious pharmaceutical value. To understand the regulatory mechanism of FIP-fve expression, we have cloned a 900 bp genomic DNA fragment from the transcriptional start site of the FIP-fve gene using genomic walker technology. Sequence analysis showed the presence of several eukaryotic transcription factor binding motifs in the 900 bp of upstream region of the FIP-fve gene, which contains one putative TATA-boxes, four possible CAAT-boxes, one ABRE, one ARE, three CGTCA-motifs, two TGA-elements and four Skn-1 motifs. The eukaryotic expression vector pfveP:: GUS-GFP was transferred into tobacco via an agrobacterium-mediated leaf disc transformation. The results showed that the FIP-fve promoter could induce the reporter gene GUS or GFP expression in different tissues of tobaccos. This study would lay a foundation for expression regulation of FIP-fve and development of genetic-modified plant products.
authors.affiliation: Shanghai Key Laboratory of Agri-biotechnology, Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, No. 800, Dongchuan Road, 200240, Shanghai, People's Republic of China.
authors.forename: Wei-Ran
authors.initials: WR
authors.lastname: Cong
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Qi-Zhang
authors.initials: QZ
authors.lastname: Li
authors.forename: Xuan-Wei
authors.initials: XW
authors.lastname: Zhou
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D001665:Binding Sites; D003001:Cloning, Molecular; D055393:Flammulina / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D048690:Fruiting Bodies, Fungal / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D007155:Immunologic Factors / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D059372:Nucleotide Motifs; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D007155:Immunologic Factors; D011994:Recombinant Proteins