title: Improved PCR-RFLP method for the identification of Escherichia coli enterohemolysin (ehxA) subtypes.
abstract: Previously published PCR-RFLP methods failed to detect the six genetically different Escherichia coli ehxA subtypes reliably. Here we describe an improved PCR-RFLP method that detects all ehxA subtypes. Importantly, ehxA-subtyping may contribute to the detection of potentially pathogenic STECs and can expedite initial screenings of patient samples and food enrichments.
authors.affiliation: U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, Division of Microbiology, College Park, MD 20740, USA; University of Hamburg, Hamburg School of Food Science, Institute of Food Chemistry, Grindelallee 117, 20146 Hamburg, Germany. Electronic address: Sandra.Lorenz@fda.hhs.gov.
authors.forename: Sandra C
authors.initials: SC
authors.lastname: Lorenz
authors.affiliation: University of Hamburg, Hamburg School of Food Science, Institute of Food Chemistry, Grindelallee 117, 20146 Hamburg, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Fischer
authors.affiliation: U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, Division of Microbiology, College Park, MD 20740, USA.
authors.forename: Julie A
authors.initials: JA
authors.lastname: Kase
mesh_terms: D004926:Escherichia coli / Q000145:classification* / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D014644:Genetic Variation; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods*; D006460:Hemolysin Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli
keywords: Escherichia coli enterohemolysin; PCR-RFLP; ehxA subtypes