title: Microfluidic electrophoretic mobility shift assays for quantitative biochemical analysis.
abstract: Electrophoretic mobility shift assays (EMSAs) play an important role in analytical chemistry, quantitative bioscience, and point-of-care diagnostics. Emerging microfluidic lab-on-a-chip technologies bring high throughput and multiplexed analysis to affinity-based electrophoretic separations, greatly advancing the performance of traditional EMSAs. This review elaborates on the relevant theoretical basis for EMSAs, surveys microfluidic-based EMSA applications in molecular conformation analyses, immunoassays, affinity assays and genomics, and outlines challenges and potential future improvements needed from this powerful assay.
authors.affiliation: Graduate Program in Bioengineering, University of California San Francisco and University of California Berkeley, CA, USA.
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Pan
authors.forename: Kelly
authors.initials: K
authors.lastname: Karns
authors.forename: Amy E
authors.initials: AE
authors.lastname: Herr
mesh_terms: D002478:Cells, Cultured; D024202:Electrophoretic Mobility Shift Assay; D023281:Genomics; D006801:Humans; D007118:Immunoassay; D046210:Microfluidic Analytical Techniques
keywords: Affinity electrophoresis; Electrophoretic mobility shift assays; Microfluidics