title: The GAR domain of GAS2L3 mediates binding to the chromosomal passenger complex and is required for localization of GAS2L3 to the constriction zone during abscission.
abstract: GAS2L3 is a recently identified tubulin- and actin-binding protein that regulates cytokinesis and abscission. In this study we show that GAS2L3 interacts with the chromosomal passenger complex (CPC), which plays key roles in mitosis and cytokinesis. Biochemical assays show that GAS2L3 directly interacts with the C-terminus of borealin and the N-terminus of survivin. We find that the interaction between these two CPC subunits and GAS2L3 is mediated by the conserved GAR domain of GAS2L3. We further show that the GAR domain of GAS2L3 is required for localization of GAS2L3 to the constriction zone. Taken together these data suggest that GAS2L3 is a downstream effector of the CPC during cytokinetic abscission.
authors.affiliation: Theodor Boveri Institute, Biocenter, and Comprehensive Cancer Center Mainfranken, University of Wuerzburg, Germany.
authors.forename: Marc
authors.initials: M
authors.lastname: Fackler
authors.forename: Patrick
authors.initials: P
authors.lastname: Wolter
authors.forename: Stefan
authors.initials: S
authors.lastname: Gaubatz
mesh_terms: D064107:Aurora Kinase B / Q000378:metabolism; D016203:CDC2 Protein Kinase / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism* / Q000502:physiology; D048749:Cytokinesis / Q000502:physiology; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism* / Q000502:physiology; D008840:Microfilament Proteins / Q000737:chemistry / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000378:metabolism*; D008938:Mitosis / Q000502:physiology; D010766:Phosphorylation; D011485:Protein Binding; D021381:Protein Transport; D013379:Substrate Specificity; D000077022:Survivin
chemical_list: D064107:Aurora Kinase B; D016203:CDC2 Protein Kinase; D018797:Cell Cycle Proteins; D051034:Inhibitor of Apoptosis Proteins; D008840:Microfilament Proteins; D008869:Microtubule-Associated Proteins; D000077022:Survivin; C107489:BIRC5 protein, human; C490157:CDCA8 protein, human; C569298:GAS2L3 protein, human; C576921:AURKB protein, human
keywords: GAS2L3; abscission; chromosomal passenger complex; cytokinesis