title: Estrogen regulates the tumour suppressor MiRNA-30c and its target gene, MTA-1, in endometrial cancer.
abstract: MicroRNA-30c (miR-30c) has been reported to be a tumour suppressor in endometrial cancer (EC). We demonstrate that miR-30c is down-regulated in EC tissue and is highly expressed in estrogen receptor (ER)-negative HEC-1-B cells. MiR-30c directly inhibits MTA-1 expression and functions as a tumour suppressor via the miR-30c-MTA-1 signalling pathway. Furthermore, miR-30c is decreased upon E2 treatment in both ER-positive Ishikawa and ER-negative HEC-1-B cells. Taken together, our results suggest that miR-30c is an important deregulated miRNA in EC and might serve as a potential biomarker and novel therapeutic target for EC.
authors.affiliation: Department of Gynecology and Obstetrics, Nanjing Drum Tower Hospital Affiliated to Nanjing University Medical School, Nanjing, China.
authors.forename: Xiangyi
authors.initials: X
authors.lastname: Kong
authors.affiliation: Department of Gynecology and Obstetrics, Nanjing Drum Tower Hospital Affiliated to Nanjing University Medical School, Nanjing, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Gynecology and Obstetrics, Nanjing Drum Tower Hospital Affiliated to Nanjing University Medical School, Nanjing, China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Department of Gynecology and Obstetrics, Nanjing Drum Tower Hospital Affiliated to Nanjing University Medical School, Nanjing, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Guo
authors.affiliation: Department of Gynecology and Obstetrics, Nanjing Drum Tower Hospital Affiliated to Nanjing University Medical School, Nanjing, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Gynecology and Obstetrics, Nanjing Drum Tower Hospital Affiliated to Nanjing University Medical School, Nanjing, China; Reproductive Medicine Center, Nanjing Drum Tower Hospital Affiliated to Nanjing University Medical School, Nanjing, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Gynecology and Obstetrics, Nanjing Drum Tower Hospital Affiliated to Nanjing University Medical School, Nanjing, China.
authors.forename: Huaijun
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Physiology, Medical College, Southeast University, Nanjing, China.
authors.forename: Qingying
authors.initials: Q
authors.lastname: Xun
mesh_terms: D000704:Analysis of Variance; D015153:Blotting, Western; D002465:Cell Movement / Q000502:physiology; D017931:DNA Primers; D016889:Endometrial Neoplasms / Q000378:metabolism*; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006655:Histone Deacetylases / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D012097:Repressor Proteins / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D013778:Tetrazolium Salts; D013844:Thiazoles; D015534:Trans-Activators
chemical_list: D017931:DNA Primers; D004967:Estrogens; C546086:MIRN30b microRNA, human; D035683:MicroRNAs; C089320:MTA1 protein, human; D012097:Repressor Proteins; D013778:Tetrazolium Salts; D013844:Thiazoles; D015534:Trans-Activators; D006655:Histone Deacetylases; C022616:thiazolyl blue