title: Dynamic monitoring of mechano-sensing of cells by gold nanoslit surface plasmon resonance sensor.
abstract: We demonstrated a real-time monitoring of live cells upon laminar shear stress stimulation via surface plasmon resonance (SPR) in gold nanoslit array. A large-area gold nanostructure consisted of 500-nm-period nanoslits was fabricated on a plastic film using the thermal-annealed template-stripping method. The SPR in the gold nanoslit array provides high surface sensitivity to monitor cell adhesion changes near the sensor surface. The human non-small cell lung cancer (CL1-0), human lung fibroblast (MRC-5), and human dermal fibroblast (Hs68) were cultured on the gold nanoslits and their dynamic responses to laminar shear stress were measured under different stress magnitudes from 0 to 30 dyne/cm(2). Cell adhesion was increased in CL1-0 under shear flow stimulation. No adhesion recovery was observed after stopping the flow. On the other hand, MRC-5 and Hs68 decreased adhesion and recovered from the shear stress. The degree of recovery was around 70% for MRC-5. This device provides dynamic study and early detection of cell adhesion changes under shear flow conditions.
authors.affiliation: Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Shu-Han
authors.initials: SH
authors.lastname: Wu
authors.affiliation: Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan.
authors.forename: Kuang-Li
authors.initials: KL
authors.lastname: Lee
authors.affiliation: Department of Mechanical and Mechatronic Engineering, National Taiwan Ocean University, Keelung, Taiwan.
authors.forename: Ruei-Hung
authors.initials: RH
authors.lastname: Weng
authors.affiliation: Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Zhao-Xian
authors.initials: ZX
authors.lastname: Zheng
authors.affiliation: Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan ; Biophotonics and Molecular Imaging Research Center (BMIRC), National Yang-Ming University, Taipei, Taiwan.
authors.forename: Arthur
authors.initials: A
authors.lastname: Chiou
authors.affiliation: Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan ; Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan ; Biophotonics and Molecular Imaging Research Center (BMIRC), National Yang-Ming University, Taipei, Taiwan.
authors.forename: Pei-Kuen
authors.initials: PK
authors.lastname: Wei
mesh_terms: D001696:Biomechanical Phenomena; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D006046:Gold / Q000737:chemistry*; D006801:Humans; D046210:Microfluidic Analytical Techniques; D049329:Nanostructures / Q000737:chemistry*; D033081:Shear Strength; D013312:Stress, Physiological; D020349:Surface Plasmon Resonance
chemical_list: D006046:Gold