title: Evaluation of fructooligosaccharides separation using a fixed-bed column packed with activated charcoal.
abstract: Recent studies have shown that the chromatographic separation of mixtures of saccharides may be improved by making use of activated charcoal, a promising low cost material for the separation of sugars, including fructooligosaccharides. In this work, the development of a methodology to separate fructooligosaccharides from glucose, fructose and sucrose, using a fixed bed column packed with activated charcoal is proposed. The influence of temperature, eluant concentration and step gradients were evaluated to increase the separation efficiency and fructooligosaccharide purity. The final degree of fructooligosaccharide purification and separation efficiency were about 94% and 3.03 respectively, using ethanol gradient concentration ranging from 3.5% to 15% (v/v) at 40°C. The fixed bed column packed with the activated charcoal was shown to be a promising alternative for sugar separation, mainly those rich in fructooligosaccharides, leading to solutions of acceptable degrees of purification.
authors.affiliation: Department of Chemical Engineering, Federal University of Santa Maria, Av. Roraima, 1000, Santa Maria, RS, CEP 97105-900, Brazil. Electronic address: raquelckuhn@yahoo.com.br.
authors.forename: Raquel Cristine
authors.initials: RC
authors.lastname: Kuhn
authors.affiliation: Department of Chemical Engineering, Federal University of Santa Maria, Av. Roraima, 1000, Santa Maria, RS, CEP 97105-900, Brazil.
authors.forename: Marcio A
authors.initials: MA
authors.lastname: Mazutti
authors.affiliation: Bioprocess Engineering Laboratory, Food Engineering Department, University of Campinas - UNICAMP, Campinas, SP, CEP 13083-862, Brazil.
authors.forename: Lilian Buoro
authors.initials: LB
authors.lastname: Albertini
authors.affiliation: Bioprocess Engineering Laboratory, Food Engineering Department, University of Campinas - UNICAMP, Campinas, SP, CEP 13083-862, Brazil.
authors.forename: Francisco Maugeri
authors.initials: FM
authors.lastname: Filho
mesh_terms: D002606:Charcoal / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D005947:Glucose / Q000302:isolation & purification; D009844:Oligosaccharides / Q000302:isolation & purification*; D013997:Time Factors
chemical_list: D009844:Oligosaccharides; C116580:fructooligosaccharide; D002606:Charcoal; D005947:Glucose