title: [miR-221 mediates epithelial-mesenchymal transition-related gene expressions via regulation of PTEN/Akt signaling in drug-resistant glioma cells].
abstract: OBJECTIVE
  To investigate the correlation between miR-221 and epithelial-mesenchymal transition (EMT) in drug-resistant glioma cells.
  
  
  METHODS
  The expression levels of miR-221, PTEN, p-Akt, E-cadherin, vimentin, and MRP1 were quantitatively analyzed in Z1 cells (primary drug-resistant cells), Z2 cells (drug-sensitive cells) and Z2-BCNU cells (drug-resistant cells) using fluorescent real-time PCR and Western blotting.
  
  
  RESULTS
  The expression levels of PTEN were significantly increased in Z2 cells compared with Z1 and Z2-BCNU cells which overexpressed miR-221 and vimentin. The expression levels of vimentin, p-Akt and MRP1 were significantly decreased in Z2 cells overexpressing E-cadherin.
  
  
  CONCLUSIONS
  MiR-221 regulates the expression of EMT-related genes through down-regulation of PTEN and activation of PI3-K/Akt signaling.
authors.affiliation: Department of Pathology, School of Medicine, Jinan University, Guangzhou 510632, China.E-mail: doctorxie@126.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xie
authors.forename: Zuoping
authors.initials: Z
authors.lastname: Huang
authors.forename: Yongrong
authors.initials: Y
authors.lastname: Yan
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Xueyun
authors.initials: X
authors.lastname: Zhong
mesh_terms: D015703:Antigens, CD; D015820:Cadherins / Q000378:metabolism; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm; D058750:Epithelial-Mesenchymal Transition; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000378:metabolism; D051059:PTEN Phosphohydrolase / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction; D014746:Vimentin / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D015820:Cadherins; D035683:MicroRNAs; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D051059:PTEN Phosphohydrolase; D051057:Proto-Oncogene Proteins c-akt; D014746:Vimentin; C513384:CDH1 protein, human; C525709:MIR221, human; C494929:PTEN protein, human; C421109:multidrug resistance-associated protein 1