title: Cloning and molecular characterization of phospholipase D (PLD) delta gene from longan (Dimocarpus longan Lour.).
abstract: Longan (Dimocarpus longan Lour.) is a non-climacteric fruit with a short postharvest life. The regulation of phospholipase D (PLD) activity closely relates to postharvest browning and senescence of longan fruit. In this study, a novel cDNA clone of longan PLDδ (LgPLDδ) was obtained and registered in GenBank (accession No. JF791814). The deduced amino acid sequence possessed all of the three typical domains of plant PLDs, a C2 domain and two catalytic HxKxxxxD motifs. The tertiary structure of LgPLDδ was further predicted. The western blot result showed that the LgPLDδ protein was specifically recognized by PLDδ antibody. The Q-RT-PCR (real-time quantitative PCR) result showed that the level of LgPLDδ mRNA expression was higher in senescent tissues than in developing tissues, which was also high in postharvest fruit. The western-blotting result further certified the different expression of LgPLDδ. These results provided a scientific basis for further investigating the mechanism of postharvest longan fruit adapting to environmental stress.
authors.affiliation: Institute of Agro-food Science & Technology, Guangxi Academy of Agricultural Sciences, Nanning, 530007, People's Republic of China.
authors.forename: Xiangrong
authors.initials: X
authors.lastname: You
authors.forename: Yayuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Zhichun
authors.initials: Z
authors.lastname: Li
authors.forename: Mingjuan
authors.initials: M
authors.lastname: Li
authors.forename: Changbao
authors.initials: C
authors.lastname: Li
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhu
authors.forename: Hongxiang
authors.initials: H
authors.lastname: Peng
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
mesh_terms: D020816:Amino Acid Motifs; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005638:Fruit / Q000201:enzymology / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010739:Phospholipase D / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D029631:Sapindaceae / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C446530:phospholipase D delta; D010739:Phospholipase D