title: A fast and selective near-infrared fluorescent sensor for multicolor imaging of biological nitroxyl (HNO).
abstract: The first near-infrared fluorescent turn-on sensor for the detection of nitroxyl (HNO), the one-electron reduced form of nitric oxide (NO), is reported. The new copper-based probe, CuDHX1, contains a dihydroxanthene (DHX) fluorophore and a cyclam derivative as a Cu(II) binding site. Upon reaction with HNO, CuDHX1 displays a five-fold fluorescence turn-on in cuvettes and is selective for HNO over thiols and reactive nitrogen and oxygen species. CuDHX1 can detect exogenously applied HNO in live mammalian cells and in conjunction with the zinc-specific, green-fluorescent sensor ZP1 can perform multicolor/multianalyte microscopic imaging. These studies reveal that HNO treatment elicits an increase in the concentration of intracellular mobile zinc.
authors.affiliation: Department of Chemistry, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.
authors.forename: Alexandra T
authors.initials: AT
authors.lastname: Wrobel
authors.forename: Timothy C
authors.initials: TC
authors.lastname: Johnstone
authors.forename: Alexandria
authors.initials: A
authors.lastname: Deliz Liang
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Lippard
authors.forename: Pablo
authors.initials: P
authors.lastname: Rivera-Fuentes
mesh_terms: D002470:Cell Survival; D003116:Color; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry*; D003300:Copper / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007259:Infrared Rays; D009589:Nitrogen Oxides / Q000378:metabolism*; D061848:Optical Imaging / Q000379:methods*; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D014966:Xanthenes / Q000737:chemistry
chemical_list: D056831:Coordination Complexes; C000607526:CuDHX1 compound; D005456:Fluorescent Dyes; D009589:Nitrogen Oxides; D009942:Organometallic Compounds; D014966:Xanthenes; D003300:Copper; C039900:nitroxyl