title: Involvement of p38 mitogen-activated protein kinase in the regulation of platelet-derived growth factor -induced cell migration.
abstract: The aim of this study was to investigate the role of p38 mitogen-activated protein kinase (MAPK) in cell migration induced by platelet-derived growth factor (PDGF). Western blot was performed to detect the phosphorylation of p38 in NIH3T3 cells treated with PDGF. A Transwell cell migration system was used to determine the effects of PDGF treatment on the migration of NIH3T3 cells and the influence of p38 deficiency on this process in a p38 gene knockout (p38(-/-)) mouse embryonic fibroblast cell line. On the stimulation of PDGF, the migration of NIH3T3 cells was significantly increased (P < 0.001) compared to the control and p38 MAP kinase was simultaneously phosphorylated. Furthermore, the PDGF-induced cell migration was significantly blocked in p38 gene knockout (p38(-/-)) mouse embryonic fibroblasts (MEFs) (P < 0.001) as compared with the wild type cells (p38(+/+)). p38 MAPK plays an important role in the regulation of cell migration induced by PDGF.
authors.affiliation: Department of Pathophysiology, Key Laboratory of Functional Proteomics of Guangdong Province, Southern Medical University, Guangzhou, 510515, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Gong
authors.forename: Jie
authors.initials: J
authors.lastname: Wei
authors.forename: Yusheng
authors.initials: Y
authors.lastname: Li
authors.forename: Weiwei
authors.initials: W
authors.lastname: Cheng
authors.forename: Peng
authors.initials: P
authors.lastname: Deng
authors.forename: Yong
authors.initials: Y
authors.lastname: Jiang