title: Modulation of subventricular zone oligodendrogenesis: a role for hemopressin?
abstract: Neural stem cells (NSCs) from the subventricular zone (SVZ) have been indicated as a source of new oligodendrocytes to use in regenerative medicine for myelin pathologies. Indeed, NSCs are multipotent cells that can self-renew and differentiate into all neural cell types of the central nervous system. In normal conditions, SVZ cells are poorly oligodendrogenic, nevertheless their oligodendrogenic potential is boosted following demyelination. Importantly, progressive restriction into the oligodendrocyte fate is specified by extrinsic and intrinsic factors, endocannabinoids being one of these factors. Although a role for endocannabinoids in oligodendrogenesis has already been foreseen, selective agonists and antagonists of cannabinoids receptors produce severe adverse side effects. Herein, we show that hemopressin (Hp), a modulator of CB1 receptors, increased oligodendroglial differentiation in SVZ neural stem/progenitor cell cultures derived from neonatal mice. The original results presented in this work suggest that Hp and derivates may be of potential interest for the development of future strategies to treat demyelinating diseases.
authors.affiliation: Center for Neuroscience and Cell Biology of Coimbra, University of Coimbra Coimbra, Portugal ; Institute of Pharmacology and Neurosciences, Faculty of Medicine, University of Lisbon Lisboa, Portugal ; Unit of Neurosciences, Institute of Molecular Medicine, University of Lisbon Lisboa, Portugal.
authors.forename: Sara
authors.initials: S
authors.lastname: Xapelli
authors.affiliation: Center for Neuroscience and Cell Biology of Coimbra, University of Coimbra Coimbra, Portugal.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Agasse
authors.affiliation: Center for Neuroscience and Cell Biology of Coimbra, University of Coimbra Coimbra, Portugal ; Institute for Stem Cell Research, Helmholtz Centre Munich, German Research Centre for Environmental Health Neuherberg, Germany ; Department of Physiological Genomics, Faculty of Medicine, Ludwig-Maximilians University of Munich Munich, Germany.
authors.forename: Sofia
authors.initials: S
authors.lastname: Grade
authors.affiliation: Center for Neuroscience and Cell Biology of Coimbra, University of Coimbra Coimbra, Portugal ; Health Sciences Research Center, University of Beira Interior Covilhã, Portugal.
authors.forename: Liliana
authors.initials: L
authors.lastname: Bernardino
authors.affiliation: Institute of Pharmacology and Neurosciences, Faculty of Medicine, University of Lisbon Lisboa, Portugal ; Unit of Neurosciences, Institute of Molecular Medicine, University of Lisbon Lisboa, Portugal.
authors.forename: Filipa F
authors.initials: FF
authors.lastname: Ribeiro
authors.affiliation: Neurochemistry Laboratory, Biophysics Institute Carlos Chagas Filho, Federal University of Rio de Janeiro Rio de Janeiro, Brazil.
authors.forename: Clarissa S
authors.initials: CS
authors.lastname: Schitine
authors.affiliation: Proteimax Biotecnologia LTDA, São Paulo SP, Brazil.
authors.forename: Andrea S
authors.initials: AS
authors.lastname: Heimann
authors.affiliation: Departamento de Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas São Paulo, Brazil.
authors.forename: Emer S
authors.initials: ES
authors.lastname: Ferro
authors.affiliation: Institute of Pharmacology and Neurosciences, Faculty of Medicine, University of Lisbon Lisboa, Portugal ; Unit of Neurosciences, Institute of Molecular Medicine, University of Lisbon Lisboa, Portugal.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Sebastião
authors.affiliation: Neurochemistry Laboratory, Biophysics Institute Carlos Chagas Filho, Federal University of Rio de Janeiro Rio de Janeiro, Brazil.
authors.forename: Ricardo A
authors.initials: RA
authors.lastname: De Melo Reis
authors.affiliation: Center for Neuroscience and Cell Biology of Coimbra, University of Coimbra Coimbra, Portugal ; Center of Investigation in Environment, Genetics and Oncobiology, Institute for Biomedical Imaging and Life Sciences, Faculty of Medicine, University of Coimbra Coimbra, Portugal.
authors.forename: João O
authors.initials: JO
authors.lastname: Malva
keywords: endocannabinoids; hemopressin; myelin pathologies; oligodendrogenesis; subventricular zone