title: Soy whey based medium for optimized phytase activity in Saccharomyces cerevisiae MTCC 5421 and α-D-galactosidase and antibacterial activities in Lactobacillus plantarum MTCC 5422 by response surface methodology.
abstract: Soy whey based growth medium for yeast enabled the native isolate of Saccharomyces cerevisiae MTCC 5421 to produce 198 U/ml of phytase activity against that of 135 U/ml in potato dextrose broth. In the same medium for lactic acid bacteria, the isolate of Lactobacillus plantarum MTCC 5422 was able to elaborate α-D-galactosidase activity of 10.6 U/ml in comparison to 6 U/ml in Lactobacillus MRS broth. Using this formulated medium, a central composite experimental design based on 5 variables of 3 factors namely incubation temperature, pH level and incubation period showed that S. cerevisiae could produce 200 U/ml of phytase in 36 h at 30 °C and pH 3.5. Similarly, L. plantarum in 33 h at 37 °C and pH 6.6 exhibited 14.2 U/ml of α-D-galactosidase activity, whereas the antibacterial activity of 14.4 AU/ml against Bacillus cereus was evident in 42 h at 42 °C and pH 6.0. The phytase and antibacterial activity units visualized in response surface plots were more or less close to those obtained with the experimental design treatments.
authors.affiliation: Human Resource Development, Central Food Technological Research Institute, Council of Scientific and Industrial Research, Mysore, 570 020 India.
authors.forename: Arekal N
authors.initials: AN
authors.lastname: Roopashri
authors.affiliation: Human Resource Development, Central Food Technological Research Institute, Council of Scientific and Industrial Research, Mysore, 570 020 India.
authors.forename: Mandyam C
authors.initials: MC
authors.lastname: Varadaraj
keywords: Antibacterial; Lactobacillus plantarum; Phytase; Saccharomyces cerevisiae; Soy whey based medium; α-D-galactosidase