title: Isolation and structure elucidation of the nucleoside antibiotic strepturidin from Streptomyces albus DSM 40763.
abstract: The antibiotic strepturidin (1) was isolated from the microorganism Streptomyces albus DSM 40763, and its structure elucidated by spectroscopic methods and chemical degradation studies. The determination of the relative and absolute stereocenters was partially achieved using chiral GC/EI-MS analysis and microderivatization by acetal ring formation and subsequent 2D-NMR analysis of key (1)H,(1)H-NOESY NMR correlations and extraction of (1)H,(13)C coupling constants from (1)H,(13)C-HMBC NMR spectra. Based on these results, a biosynthesis model was proposed.
authors.affiliation: Institut für Chemie, Technische Universität Berlin, Berlin, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Pesic
authors.affiliation: Helmholtz Zentrum für Infektionsforschung, Braunschweig, Germany.
authors.forename: Britta
authors.initials: B
authors.lastname: Steinhaus
authors.affiliation: Institut für Chemie, Technische Universität Berlin, Berlin, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Kemper
authors.affiliation: Institut für Chemie, Technische Universität Berlin, Berlin, Germany.
authors.forename: Jonny
authors.initials: J
authors.lastname: Nachtigall
authors.affiliation: Technische Hochschule Darmstadt, Institut für Mikrobiologie und Genetik, Darmstadt, Germany.
authors.forename: Hans Jürgen
authors.initials: HJ
authors.lastname: Kutzner
authors.affiliation: Helmholtz Zentrum für Infektionsforschung, Braunschweig, Germany.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Höfle
authors.affiliation: Institut für Chemie, Technische Universität Berlin, Berlin, Germany.
authors.forename: Roderich D
authors.initials: RD
authors.lastname: Süssmuth
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification*; D009705:Nucleosides / Q000737:chemistry* / Q000302:isolation & purification*; D013302:Streptomyces / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D009705:Nucleosides