title: The improved resistance to high salinity induced by trehalose is associated with ionic regulation and osmotic adjustment in Catharanthus roseus.
abstract: The effects of exogenous trehalose (Tre) on salt tolerance of pharmaceutical plant Catharanthus roseus and the physiological mechanisms were both investigated in this study. The results showed that the supplement of Tre in saline condition (250 mM NaCl) largely alleviated the inhibitory effects of salinity on plant growth, namely biomass accumulation and total leaf area per plant. In this saline condition, the decreased level of relative water content (RWC) and photosynthetic rate were also greatly rescued by exogenous Tre. This improved performance of plants under high salinity induced by Tre could be partly ascribed to its ability to decrease accumulation of sodium, and increase potassium in leaves. The exogenous Tre led to high levels of fructose, glucose, sucrose and Tre inside the salt-stressed plants during whole the three-week treatment. The major free amino acids such as proline, arginine, threonine and glutamate were also largely elevated in the first two-week course of treatment with Tre in saline solution. It was proposed here that Tre might act as signal to make the salt-stressed plants actively increase internal compatible solutes, including soluble sugars and free amino acids, to control water loss, leaf gas exchange and ionic flow at the onset of salt stress. The application of Tre in saline condition also promoted the accumulation of alkaloids. The regulatory role of Tre in improving salt tolerance was optimal with an exogenous concentration of 10 mM Tre. Larger concentrations of Tre were supra-optimum and adversely affected plant growth.
authors.affiliation: Department of Genetics, College of Life Science, Northeast Forestry University, Harbin 150040, China; Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Harbin 150040, China.
authors.forename: Bowen
authors.initials: B
authors.lastname: Chang
authors.affiliation: Shanghai Chenshan Plant Science Research Center, Chinese Academy of Sciences, Shanghai Chenshan Botanical Garden, Shanghai 201602, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Food and Drug Administration of QuFu City, QuFu, ShanDong Province 273100, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Cong
authors.affiliation: Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Harbin 150040, China.
authors.forename: Yuangang
authors.initials: Y
authors.lastname: Zu
authors.affiliation: Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Harbin 150040, China. Electronic address: tangzh@nefu.edu.cn.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Tang
mesh_terms: D000470:Alkaloids / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D018533:Biomass; D050260:Carbohydrate Metabolism / Q000187:drug effects; D030008:Catharanthus / Q000187:drug effects* / Q000378:metabolism; D007477:Ions / Q000378:metabolism*; D064587:Osmoregulation / Q000187:drug effects*; D009995:Osmosis; D009997:Osmotic Pressure; D010788:Photosynthesis; D018515:Plant Leaves / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D054712:Salinity; D055049:Salt Tolerance / Q000187:drug effects*; D012964:Sodium / Q000378:metabolism; D012965:Sodium Chloride / Q000009:adverse effects* / Q000378:metabolism; D013312:Stress, Physiological / Q000187:drug effects; D014199:Trehalose / Q000378:metabolism / Q000494:pharmacology*; D014867:Water / Q000378:metabolism
chemical_list: D000470:Alkaloids; D000596:Amino Acids; D007477:Ions; D014867:Water; D012965:Sodium Chloride; D012964:Sodium; D014199:Trehalose; D011188:Potassium
keywords: Alkaloids; Catharanthus roseus; Ionic regulation; Osmotic adjustment; Salt tolerance; Trehalose