title: Graphene oxide based magnetic nanocomposites for efficient treatment of breast cancer.
abstract: The present work reports a simple one step synthesis of nanoscale graphene oxide magnetic composites (GO-IO) using ferrofluid (GO-IOF). The obtained GO-IO were compared with GO-IO obtained from in situ (GO-IOI) methods. Anastrozole (ANS) was loaded on the GO-IOI and GO-IOF via simple stirring method to form GO-IOA and GO-IOFA respectively. These GO-IO prepared by two techniques were characterized using spectroscopic techniques and vibrating sample magnetometer (VSM) analysis. Particle size and potential were measured using Malvern Zetasizer. Scanning electron microscopy (SEM) was used for studying the surface morphology of GO-IO, and in addition to this elemental analysis was also performed for confirming the presence of iron. The cell viability assay was carried out using the MCF-7 cell line. It revealed that GO-IOFA had reasonably high cytotoxicity (49.7%) compared to GO (13.1%), ANS (16.6), GO-IOI (13%), GO-IOF (13.6) and GO-IOIA (18.34%). Both, GO-IOIA and GO-IOFA showed improved cytotoxicity when compared with pure ANS. GO-IOF were found to exhibit superior magnetic activity due to higher iron content along with smaller particle size and higher loading efficiency compared to GO-IOI. The overall effect suggests that GO-IO can be utilized as efficient carriers for the loading of chemotherapeutic agents.
authors.affiliation: Department of Pharmaceutics, H. R. Patel Institute of Pharmaceutical Education and Research, Karwand Naka, Shirpur. Dist., Dhule 425 405, Maharashtra, India.
authors.forename: Nilesh S
authors.initials: NS
authors.lastname: Chaudhari
authors.affiliation: Department of Pharmaceutics, H. R. Patel Institute of Pharmaceutical Education and Research, Karwand Naka, Shirpur. Dist., Dhule 425 405, Maharashtra, India.
authors.forename: Abhijeet P
authors.initials: AP
authors.lastname: Pandey
authors.affiliation: Department of Pharmaceutics, H. R. Patel Institute of Pharmaceutical Education and Research, Karwand Naka, Shirpur. Dist., Dhule 425 405, Maharashtra, India.
authors.forename: Pravin O
authors.initials: PO
authors.lastname: Patil
authors.affiliation: Department of Pharmaceutics, Rajarshi Shahu College of Pharmacy and Research, Tathawade, Pune 411 033, Maharashtra, India.
authors.forename: Avinash R
authors.initials: AR
authors.lastname: Tekade
authors.affiliation: Department of Pharmaceutics, H. R. Patel Institute of Pharmaceutical Education and Research, Karwand Naka, Shirpur. Dist., Dhule 425 405, Maharashtra, India.
authors.forename: Sanjay B
authors.initials: SB
authors.lastname: Bari
authors.affiliation: Department of Pharmaceutics, H. R. Patel Institute of Pharmaceutical Education and Research, Karwand Naka, Shirpur. Dist., Dhule 425 405, Maharashtra, India. Electronic address: pkdesh@rediffmail.com.
authors.forename: Prashant K
authors.initials: PK
authors.lastname: Deshmukh
mesh_terms: D000077384:Anastrozole; D018931:Antineoplastic Agents, Hormonal / Q000008:administration & dosage / Q000737:chemistry* / Q000633:toxicity; D001943:Breast Neoplasms / Q000188:drug therapy; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry*; D005260:Female; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D061986:MCF-7 Cells; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D009570:Nitriles / Q000008:administration & dosage / Q000737:chemistry* / Q000633:toxicity; D010087:Oxides / Q000737:chemistry; D010316:Particle Size; D014230:Triazoles / Q000008:administration & dosage / Q000737:chemistry* / Q000633:toxicity
chemical_list: D018931:Antineoplastic Agents, Hormonal; D004337:Drug Carriers; D058185:Magnetite Nanoparticles; D009570:Nitriles; D010087:Oxides; D014230:Triazoles; D000077384:Anastrozole; D006108:Graphite
keywords: Anastrozole; Breast cancer; Ferrofluid; MCF-7