title: Effect of ultrasonic extraction conditions on antioxidative and immunomodulatory activities of a Ganoderma lucidum polysaccharide originated from fermented soybean curd residue.
abstract: A crude Ganoderma lucidum polysaccharide (GLPL) was extracted from fermented soybean curd residue by ultrasonic assisted extraction. The optimal extraction conditions were 30 min at 80 °C with 80 W and water to solid ratio of 10, and with this method 115.47 ± 2.95 mg/g of GLPL yield was obtained. Additionally, the antioxidant and immunomodulatory activities of GLPL were investigated. The results showed that GLPL exhibited strong antioxidant effects, which included scavenging activities against DPPH radicals, hydrogen oxide and ABTS radicals with IC50 values of 0.23, 0.48 and 0.69 mg/mL, respectively. For immunomodulatory activities, GLPL was shown to strongly stimulate the proliferation of macrophages (158.02 ± 13.12%), the production of nitric oxide and phagocytosis (21.16 ± 1.65 μM), and, at 40.00 μg/mL, protected macrophage from Doxorubicin (DOX) (0.16 ± 0.003).
authors.affiliation: Graduate School of Life and Environmental Science, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.
authors.forename: Min
authors.initials: M
authors.lastname: Shi
authors.affiliation: Graduate School of Life and Environmental Science, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan. Electronic address: yo.innan.fu@u.tsukuba.ac.jp.
authors.forename: Yingnan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Graduate School of Life and Environmental Science, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.
authors.forename: Xuansheng
authors.initials: X
authors.lastname: Hu
authors.affiliation: Graduate School of Life and Environmental Science, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.
authors.forename: Zhenya
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D005285:Fermentation; D032902:Ganoderma / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D006801:Humans; D007155:Immunologic Factors / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects; D011134:Polysaccharides / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D045730:Soy Foods / Q000382:microbiology*; D013025:Glycine max / Q000382:microbiology; D014465:Ultrasonics / Q000379:methods*; D014866:Waste Products / Q000032:analysis
chemical_list: D000975:Antioxidants; D007155:Immunologic Factors; D011134:Polysaccharides; D014866:Waste Products
keywords: Antioxidant activities; Ganoderma lucidum; Immunomodulation activities; Polysaccharide; Soybean curd residue; Ultrasonic assisted extraction