title: [DNA image-fluorimetry of individual human chromosomes].
abstract: Mucrofluorimetric method for the determination of DNA content in individual human chromosomes has been developed. The method is based on a preliminary identification of chromosomes with Hoechst 33258, followed by staining of the chromosomes with Feulgen reaction using Schiffs reagent type ethidium bromide-SO2, then measuring the fluorescence intensity of the chromosomes using an image analyzer. The method allows to determine the DNA content of individual chromosomes with accuracy up to 4.5 fg. DNA content of individual human chromosomes, their p-and q-arms as well as homologous chromosomes were measured using the developed method. It has been shown that the DNA content in the chromosomes of normal human karyotype is unstable. Fluctuations in the DNA content in some chromosomes can vary 35-40 fg.
authors.forename: N A
authors.initials: NA
authors.lastname: Agafonova
authors.forename: G A
authors.initials: GA
authors.lastname: Sakuta
authors.forename: Iu M
authors.initials: IuM
authors.lastname: Rozanov
authors.forename: G I
authors.initials: GI
authors.lastname: Shteĭn
authors.forename: B N
authors.initials: BN
authors.lastname: Kudriavtsev
mesh_terms: D006690:Bisbenzimidazole; D002871:Chromosome Banding; D002877:Chromosomes, Human / Q000648:ultrastructure*; D004247:DNA / Q000648:ultrastructure*; D004996:Ethidium / Q000737:chemistry; D005434:Flow Cytometry; D005453:Fluorescence; D005470:Fluorometry / Q000379:methods*; D006801:Humans; D007621:Karyotyping; D057054:Molecular Imaging / Q000379:methods*
chemical_list: D004247:DNA; D004996:Ethidium; D006690:Bisbenzimidazole