title: An investigation into membrane bound redox carriers involved in energy transduction mechanism in Brevibacterium linens DSM 20158 with unsequenced genome.
abstract: Brevibacterium linens (B. linens) DSM 20158 with an unsequenced genome can be used as a non-pathogenic model to study features it has in common with other unsequenced pathogens of the same genus on the basis of comparative proteome analysis. The most efficient way to kill a pathogen is to target its energy transduction mechanism. In the present study, we have identified the redox protein complexes involved in the electron transport chain of B. linens DSM 20158 from their clear homology with the shot-gun genome sequenced strain BL2 of B. linens by using the SDS-Polyacrylamide gel electrophoresis coupled with nano LC-MS/MS mass spectrometry. B. linens is found to have a branched electron transport chain (Respiratory chain), in which electrons can enter the respiratory chain either at NADH (Complex I) or at Complex II level or at the cytochrome level. Moreover, we are able to isolate, purify, and characterize the membrane bound Complex II (succinate dehydrogenase), Complex III (menaquinone cytochrome c reductase cytochrome c subunit, Complex IV (cytochrome c oxidase), and Complex V (ATP synthase) of B. linens strain DSM 20158.
authors.affiliation: Department of Chemistry, GC University Lahore, Lahore, 54000, Pakistan.
authors.forename: Khadija
authors.initials: K
authors.lastname: Shabbiri
authors.forename: Catherine H
authors.initials: CH
authors.lastname: Botting
authors.forename: Ahmad
authors.initials: A
authors.lastname: Adnan
authors.forename: Matthew
authors.initials: M
authors.lastname: Fuszard
authors.forename: Shahid
authors.initials: S
authors.lastname: Naseem
authors.forename: Safeer
authors.initials: S
authors.lastname: Ahmed
authors.forename: Shahida
authors.initials: S
authors.lastname: Shujaat
authors.forename: Quratulain
authors.initials: Q
authors.lastname: Syed
authors.forename: Waqar
authors.initials: W
authors.lastname: Ahmad
mesh_terms: D025181:ATP Synthetase Complexes / Q000737:chemistry / Q000302:isolation & purification; D000244:Adenosine Diphosphate / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D001951:Brevibacterium / Q000737:chemistry* / Q000235:genetics; D045304:Cytochromes c / Q000737:chemistry / Q000302:isolation & purification; D045222:Electron Transport Chain Complex Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D004735:Energy Transfer; D016680:Genome, Bacterial; D007700:Kinetics; D010084:Oxidation-Reduction; D010710:Phosphates / Q000737:chemistry; D024482:Vitamin K 2 / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D045222:Electron Transport Chain Complex Proteins; D010710:Phosphates; D024482:Vitamin K 2; D000244:Adenosine Diphosphate; D045304:Cytochromes c; D025181:ATP Synthetase Complexes