title: Futsal and continuous exercise induce similar changes in specific skeletal muscle signalling proteins.
abstract: Exercise elicits skeletal-muscle adaptations which are important for improved health outcomes. We compared the effects of a futsal game (FUT) and moderate-intensity continuous exercise (MOD), on the skeletal-muscle protein signalling responses in young, healthy individuals. 16 men undertook an incremental exercise test and a resting muscle biopsy performed >48 h apart. They were then randomly allocated to either FUT (n=12) consisting of 2 x 20 min halves, or MOD (n=8) consisting of a work-matched running bout performed at an intensity corresponding to the individual ventilatory threshold 1. Work matching was achieved by means of triaxial accelerometers. Immediately after FUT and MOD, participants underwent a second biopsy to assess exercise-induced changes in protein signalling. Total and phosphorylated protein abundance was assessed via western blotting. Both FUT and MOD altered signalling responses in skeletal muscle. FUT increased total ATF2 protein abundance (p=0.048) and phosphorylation (p=0.029), while no changes occurred with MOD. Both exercise regimes increased ACC phosphorylation (p=0.01) and returned a trend for increased p38MAPK phosphorylation. Futsal may be employed as an alternative to continuous exercise to elicit muscle adaptations which may be associated with improved health outcomes. As only FUT increased ATF2 activation, this protein might be a target of future investigation on exercise-induced signalling.
authors.affiliation: Institute of Sport, Exercise and Active Living (ISEAL), College of Sport and Exercise Science, Victoria University, Melbourne, Australia.
authors.forename: F R
authors.initials: FR
authors.lastname: Serpiello
authors.affiliation: Institute of Sport, Exercise and Active Living (ISEAL), College of Sport and Exercise Science, Victoria University, Melbourne, Australia.
authors.forename: M J
authors.initials: MJ
authors.lastname: McKenna
authors.affiliation: Department of Neurological, Neuropsychological, Morphological and -Movement Sciences, Faculty of Sport Sciences, University of Verona, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Coratella
authors.affiliation: The University of Auckland, Liggins Institute, Auckland, New Zealand.
authors.forename: J F
authors.initials: JF
authors.lastname: Markworth
authors.affiliation: Department of Neurological, Neuropsychological, Morphological and -Movement Sciences, Faculty of Sport Sciences, University of Verona, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Tarperi
authors.affiliation: Institute of Sport, Exercise and Active Living (ISEAL), College of Sport and Exercise Science, Victoria University, Melbourne, Australia.
authors.forename: D
authors.initials: D
authors.lastname: Bishop
authors.affiliation: Institute of Sport, Exercise and Active Living (ISEAL), College of Sport and Exercise Science, Victoria University, Melbourne, Australia.
authors.forename: N K
authors.initials: NK
authors.lastname: Stepto
authors.affiliation: The University of Auckland, Liggins Institute, Auckland, New Zealand.
authors.forename: D
authors.initials: D
authors.lastname: Cameron-Smith
authors.affiliation: Department of Neurological, Neuropsychological, Morphological and -Movement Sciences, Faculty of Sport Sciences, University of Verona, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Schena
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D051698:Activating Transcription Factor 2 / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D001706:Biopsy; D005080:Exercise Test; D006801:Humans; D008297:Male; D009124:Muscle Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D010766:Phosphorylation; D012420:Running / Q000502:physiology*; D015398:Signal Transduction; D012916:Soccer / Q000502:physiology*; D055815:Young Adult; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: C497105:ATF2 protein, human; D051698:Activating Transcription Factor 2; D009124:Muscle Proteins; D048051:p38 Mitogen-Activated Protein Kinases; D000103:Acetyl-CoA Carboxylase