title: [Identification and characterization of enterovirus 71 isolated in Yunnan province].
abstract: OBJECTIVE
  Identification and characterization of eight enterovirus 71 samples isolated from Yunnan province.
  
  
  METHODS
  Collecting specimens from cases of Hand, Foot and Mouth Disease (HFMD), samples were identificated by RT-PCR, isolated the virus with cells. The isolating virus passaged in vero cells, and the vpl genes sequencing. Immunization the mice with the virus, neutralization test and cross-protection test were conducted with those antisera.
  
  
  RESULTS
  All samples were identified as EV71, and eight strains had cytopathic effect (CPE) in vero cells, some antisera titers were more than 1:10,000.
  
  
  CONCLUSIONS
  Eight strains of the virus grow well in vero cell, antiserum showed protection against EV71 A and C4 genotype.
authors.affiliation: Yunnan Center for Disease Control, Kunming 650022, China.
authors.forename: Shi-Wen
authors.initials: SW
authors.lastname: Zhao
authors.affiliation: Yunnan Center for Disease Control, Kunming 650022, China.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Yin
authors.affiliation: Yunnan Center for Disease Control, Kunming 650022, China.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Cun
authors.affiliation: Yunnan Center for Disease Control, Kunming 650022, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Gao
authors.affiliation: Yunnan Center for Disease Control, Kunming 650022, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yin
authors.affiliation: Yunnan Center for Disease Control, Kunming 650022, China.
authors.forename: Guo-Jun
authors.initials: GJ
authors.lastname: Liu
authors.affiliation: Yunnan Center for Disease Control, Kunming 650022, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Xu
mesh_terms: D000818:Animals; D002681:China / Q000453:epidemiology; D002522:Chlorocebus aethiops; D029821:Enterovirus A, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D006232:Hand, Foot and Mouth Disease / Q000453:epidemiology / Q000821:virology*; D006801:Humans; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D010802:Phylogeny; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins