title: A proneural gene controls C. elegans neuroblast asymmetric division and migration.
abstract: Proneural genes control the generation of neuroblasts from the neuroepithelium, but their functions in neuroblast asymmetric division and migration remain elusive. Here, we identified Caenorhabditiselegans mutants of a proneural transcription factor (TF) lin-32, in which Q neuroblasts are produced. We showed that LIN-32 functions in parallel with a storkhead TF, HAM-1, to regulate Q neuroblast asymmetric division, and that Q neuroblast migration is inhibited in lin-32 alleles. Consistently, lin-32 is expressed throughout Q neuroblast lineage, suggesting that LIN-32 may promote different target gene expression. Our studies thus uncovered previously unknown functions of a proneural gene in neuroblast development.
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China.
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Liu
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Peishan
authors.initials: P
authors.lastname: Yi
authors.affiliation: Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Tian
authors.affiliation: Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Yongping
authors.initials: Y
authors.lastname: Chai
authors.affiliation: School of Medicine, Tsinghua University, Beijing 100084, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China. Electronic address: guangshuo.ou@gmail.com.
authors.forename: Guangshuo
authors.initials: G
authors.lastname: Ou
mesh_terms: D000483:Alleles; D000818:Animals; D060049:Asymmetric Cell Division; D017173:Caenorhabditis elegans / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics* / Q000378:metabolism; D002465:Cell Movement; D048429:Cell Size; D018507:Gene Expression Regulation, Developmental; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000502:physiology*; D059008:Time-Lapse Imaging; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D029742:Caenorhabditis elegans Proteins; C567963:LIN-32 protein, C elegans; D014157:Transcription Factors
keywords: Asymmetric cell division; C. elegans; Cell migration; Neuroblast; Proneural Gene