title: MiR-29a/b/c regulate human circadian gene hPER1 expression by targeting its 3'UTR.
abstract: Several essential biological progresses in mammals are regulated by circadian rhythms. Though the molecular mechanisms of oscillating these circadian rhythms have been uncovered, the specific functions of the circadian genes are not very clear. It has been reported that knocking down circadian genes by microRNA is a useful strategy to explore the function of the circadian rhythms. In this study, through a forward bioinformatics screening approach, we identified miR-29a/b/c as potent inhibitors for the human circadian gene hPER1. We further found that miR-29a/b/c could directly target hPER1 3'untranslated region (UTR) and down-regulate hPER1 at both mRNA and protein expression levels in human A549 cells. Thus, our findings suggested that the expression of hPER1 is regulated by miR-29a/b/c, which may also provide a new clue for the function of hPER1.
authors.affiliation: Health Ministry Key Laboratory of Chronobiology, Pre-clinic and Forensic Medical School, Sichuan University, Chengdu 610041, China.
authors.forename: Xiyan
authors.initials: X
authors.lastname: Zhao
authors.forename: Xueqiang
authors.initials: X
authors.lastname: Zhu
authors.forename: Shuting
authors.initials: S
authors.lastname: Cheng
authors.forename: Yizhou
authors.initials: Y
authors.lastname: Xie
authors.forename: Zhengrong
authors.initials: Z
authors.lastname: Wang
authors.forename: Yanyou
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhou
authors.initials: Z
authors.lastname: Jiang
authors.forename: Jing
authors.initials: J
authors.lastname: Xiao
authors.forename: Huiling
authors.initials: H
authors.lastname: Guo
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Wang
mesh_terms: D020413:3' Untranslated Regions; D000465:Algorithms; D001483:Base Sequence; D015153:Blotting, Western; D045744:Cell Line, Tumor; D017931:DNA Primers; D015536:Down-Regulation; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D056950:Period Circadian Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D020413:3' Untranslated Regions; D017931:DNA Primers; C532045:MIRN29a microRNA, human; D035683:MicroRNAs; C539022:PER1 protein, human; D056950:Period Circadian Proteins
keywords: 3′UTR; circadian rhythms; hPER1; miR-29a/b/c