title: Cytotoxic activity of two natural sesquiterpene lactones, isobutyroylplenolin and arnicolide D, on human colon cancer cell line HT-29.
abstract: In this study, we found that two sesquiterpene lactones, isobutyroylplenolin and arnicolide D, from Centipeda minima L. (Compositae) exerted stronger cytotoxic activity than cisplatin on the human colon carcinoma HT-29 cell line. Furthermore, the cytotoxicity of these two compounds on normal cells was weaker than that of cisplatin. Treatment with isobutyroylplenolin and arnicolide D increased the levels of intracellular reactive oxygen species and decreased the levels of nuclear factor-κB protein, resulting in cell cycle arrest in G1 phase and apoptosis. We also discuss the difference in structure and activity between these two compounds.
authors.affiliation: a School of Pharmaceutical Sciences, Kitasato University , 5-9-1 Shirokane, Minato-ku , Tokyo 108-0086 , Japan.
authors.forename: Xuedan
authors.initials: X
authors.lastname: Huang
authors.forename: Yurika
authors.initials: Y
authors.lastname: Awano
authors.forename: Eri
authors.initials: E
authors.lastname: Maeda
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Asada
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Takemoto
authors.forename: Takashi
authors.initials: T
authors.lastname: Watanabe
authors.forename: Akiko
authors.initials: A
authors.lastname: Kojima-Yuasa
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kobayashi
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D019659:Asteraceae / Q000737:chemistry*; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology; D003110:Colonic Neoplasms / Q000188:drug therapy*; D016193:G1 Phase / Q000187:drug effects; D019073:HT29 Cells; D006801:Humans; D007783:Lactones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D015394:Molecular Structure; D016328:NF-kappa B / Q000032:analysis; D017382:Reactive Oxygen Species / Q000032:analysis; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D007783:Lactones; D016328:NF-kappa B; D017382:Reactive Oxygen Species; D012717:Sesquiterpenes; C000592536:arnicolide D; C000592535:isobutyroylplenolin; D002945:Cisplatin
keywords: Centipeda minima L; arnicolide D; cytotoxic; isobutyroylplenolin; nuclear factor-κB; reactive oxygen species