title: Airborne acrolein induces keratin-8 (Ser-73) hyperphosphorylation and intermediate filament ubiquitination in bronchiolar lung cell monolayers.
abstract: The combustion product acrolein is a key mediator of pulmonary edema in victims of smoke inhalation injury. Since studying acrolein toxicity in conventional in vitro systems is complicated by reactivity with nucleophilic culture media constituents, we explored an exposure system which delivers airborne acrolein directly to lung cell monolayers at the air-liquid interface. Calu-3 lung adenocarcinoma cells were maintained on membrane inserts such that the basal surface was bathed in nucleophile-free media while the upper surface remained in contact with acrolein-containing air. Cells were exposed to airborne acrolein for 30 min before they were allowed to recover in fresh media, with cell sampling at defined time points to allow evaluation of toxicity and protein damage. After prior exposure to acrolein, cell ATP levels remained close to controls for 4h but decreased in an exposure-dependent manner by 24h. A loss of transepithelial electrical resistance and increased permeability to fluorescein isothiocyanate-labeled dextran preceded ATP loss. Use of antibody arrays to monitor protein expression in exposed monolayers identified strong upregulation of phospho-keratin-8 (Ser(73)) as an early consequence of acrolein exposure. These changes were accompanied by chemical damage to keratin-8 and other intermediate filament family members, while acrolein exposure also resulted in controlled ubiquitination of high mass proteins within the intermediate filament extracts. These findings confirm the usefulness of systems allowing delivery of airborne smoke constituents to lung cell monolayers during studies of the molecular basis for acute smoke intoxication injury.
authors.affiliation: Pharmacology and Anaesthesiology Unit, School of Medicine & Pharmacology, The University of Western Australia, Nedlands, WA 6009, Australia. Electronic address: philip.burcham@uwa.edu.au.
authors.forename: Philip C
authors.initials: PC
authors.lastname: Burcham
authors.affiliation: Pharmacology and Anaesthesiology Unit, School of Medicine & Pharmacology, The University of Western Australia, Nedlands, WA 6009, Australia.
authors.forename: Albert
authors.initials: A
authors.lastname: Raso
authors.affiliation: Pharmacology and Anaesthesiology Unit, School of Medicine & Pharmacology, The University of Western Australia, Nedlands, WA 6009, Australia.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Henry
mesh_terms: D000171:Acrolein / Q000633:toxicity*; D000393:Air Pollutants / Q000633:toxicity*; D001980:Bronchi / Q000166:cytology; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D007382:Intermediate Filaments / Q000378:metabolism*; D053533:Keratin-8 / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D054875:Ubiquitination; D014746:Vimentin / Q000378:metabolism*
chemical_list: D000393:Air Pollutants; D055551:HSP27 Heat-Shock Proteins; D053533:Keratin-8; D014746:Vimentin; D000171:Acrolein
keywords: Acrolein; Air–liquid interface; Keratin phosphorylation; Lung injury; Protein adducts; Ubiquitination