title: Comparative effects of chlorhexidine and essential oils containing mouth rinse on stem cells cultured on a titanium surface.
abstract: Chlorhexidine (CHX) and Listerine (LIS), an essential oil compound, are the two commonly used adjunctive agents for mechanical debridement, for reducing the bacterial load in the treatment of peri-implant inflammation. However, antimicrobial agents have been reported to be cytotoxic to the alveolar bone cells and gingival epithelial cells. The present study was performed to examine the effects of antiseptics CHX and LIS, on the morphology and proliferation of stem cells. Stem cells derived from the buccal fat pad were grown on machined titanium discs. Each disc was immersed in CHX or LIS for 30 sec, 1.5 min or 4.5 min. Cell morphology was evaluated with a confocal laser microscope and the viability of the cells was quantitatively analyzed with the cell counting kit-8 (CCK-8). The untreated cells attached to the titanium discs demonstrated well-organized actin cytoskeletons. No marked alterations in the cytoskeletal organization were observed in any of the treated groups. The treatment with CHX and LIS of the titanium discs decreased the viability of the cells grown on the treated discs (P<0.05). The stem cells derived from the buccal fat pad were sensitive to CHX and LIS, and a reduction in cellular viability was observed when these agents were applied to the discs for 30 sec. Further studies are required to determine the optimal application time and concentration of this antimicrobial agent for maximizing the reduction of the bacterial load and minimizing the cytotoxicity to the surrounding cells.
authors.affiliation: Department of Periodontics, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea.
authors.forename: Jun-Beom
authors.initials: JB
authors.lastname: Park
authors.affiliation: Department of Dentistry, Graduate School, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea.
authors.forename: Gil
authors.initials: G
authors.lastname: Lee
authors.affiliation: Department of Dentistry, Graduate School, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea.
authors.forename: Byeong Gon
authors.initials: BG
authors.lastname: Yun
authors.affiliation: Department of Oral and Maxillofacial Surgery, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea.
authors.forename: Chang-Hyen
authors.initials: CH
authors.lastname: Kim
authors.affiliation: Department of Periodontics, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea.
authors.forename: Youngkyung
authors.initials: Y
authors.lastname: Ko
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002710:Chlorhexidine / Q000494:pharmacology*; D004338:Drug Combinations; D006801:Humans; D009055:Mouth / Q000166:cytology; D009067:Mouthwashes / Q000494:pharmacology*; D009822:Oils, Volatile / Q000494:pharmacology*; D012459:Salicylates / Q000494:pharmacology*; D013234:Stem Cells / Q000166:cytology*; D013499:Surface Properties; D013729:Terpenes / Q000494:pharmacology*; D014025:Titanium / Q000494:pharmacology*
chemical_list: D004338:Drug Combinations; D009067:Mouthwashes; D009822:Oils, Volatile; D012459:Salicylates; D013729:Terpenes; C057473:Listerine; D014025:Titanium; D002710:Chlorhexidine