title: Effects of the polymeric niche on neural stem cell characteristics during primary culturing.
abstract: The polymeric niche encountered by cells during primary culturing can affect cell fate. However, most cell types are primarily propagated on polystyrene (PS). A cell type specific screening for optimal primary culture polymers particularly for regenerative approaches seems inevitable. The effect of physical and chemical properties of treated (corona, oxygen/nitrogen plasma) and untreated cyclic olefin polymer (COP), polymethymethacrylate (PMMA), PP, PLA, PS, PC on neuronal stem cell characteristics was analyzed. Our comprehensive approach revealed plasma treated COP and PMMA as optimal polymers for primary neuronal stem cell culturing and propagation. An increase in the number of NT2/D1 cells with pronounced adhesion, metabolic activities and augmented expression of neural precursor markers was associated to the plasma treatment of surfaces of COP and PMMA with nitrogen or oxygen, respectively. A shift towards large cell sizes at stable surface area/volume ratios that might promote the observed increase in metabolic activities and distinct modulations in F-actin arrangements seem to be primarily mediated by the plasma treatment of surfaces. These results indicate that the polymeric niche has a distinct impact on various cell characteristics. The selection of distinct polymers and the controlled design of an optimized polymer microenvironment might thereby be an effective tool to promote essential cell characteristics for subsequent approaches.
authors.affiliation: Research Unit for Experimental Neurotraumatology, Department of Neurosurgery, Medical University of Graz, Auenbruggerplatz 2/2, 8036, Graz, Austria.
authors.forename: Stefan
authors.initials: S
authors.lastname: Haubenwallner
authors.forename: Matthias
authors.initials: M
authors.lastname: Katschnig
authors.forename: Ulrike
authors.initials: U
authors.lastname: Fasching
authors.forename: Silke
authors.initials: S
authors.lastname: Patz
authors.forename: Christa
authors.initials: C
authors.lastname: Trattnig
authors.forename: Natascha
authors.initials: N
authors.lastname: Andraschek
authors.forename: Gerda
authors.initials: G
authors.lastname: Grünbacher
authors.forename: Markus
authors.initials: M
authors.lastname: Absenger
authors.forename: Stephan
authors.initials: S
authors.lastname: Laske
authors.forename: Clemens
authors.initials: C
authors.lastname: Holzer
authors.forename: Werner
authors.initials: W
authors.lastname: Balika
authors.forename: Manuela
authors.initials: M
authors.lastname: Wagner
authors.forename: Ute
authors.initials: U
authors.lastname: Schäfer
mesh_terms: D000818:Animals; D061249:Batch Cell Culture Techniques / Q000379:methods*; D001672:Biocompatible Materials / Q000737:chemistry*; D002460:Cell Line; D002478:Cells, Cultured; D008422:Materials Testing; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology* / Q000502:physiology*; D011108:Polymers / Q000737:chemistry*; D055153:Stem Cell Niche / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D011108:Polymers