title: Surface-mediated protein disaggregation.
abstract: Preventing protein aggregation is of both biological and industrial importance. Interprotein interactions between the hydrophobic residues of the protein are known to be the major driving force for protein aggregation. In this article, we show how surface chemistry and curvature can be tuned to mitigate these interprotein interactions. Our results calculated in the framework of the Hydrophobic-Polar (HP) lattice model show that interprotein interactions can be drastically reduced by increasing the surface hydrophobicity to a critical value corresponding to the adsorption transition of the protein. At this value of surface hydrophobicity, proteins lose interprotein contacts to gain surface contacts, and thus the surface helps to reduce the interprotein interactions. Furthermore, we show that the adsorption of the proteins inside hydrophobic pores of optimal sizes are most efficient at both reducing interprotein contacts and simultaneously retaining most of the native contacts probably as a result of confinement-induced stabilization.
authors.affiliation: Department of Chemical Engineering, Columbia University , New York, New York 10027, United States.
authors.forename: Mithun
authors.initials: M
authors.lastname: Radhakrishna
authors.forename: Sanat K
authors.initials: SK
authors.lastname: Kumar
mesh_terms: D000327:Adsorption; D057927:Hydrophobic and Hydrophilic Interactions; D056004:Molecular Dynamics Simulation; D009010:Monte Carlo Method; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties; D013696:Temperature
chemical_list: D011506:Proteins