title: Cellular and genomic toxicity produced by UV light in Chinese hamster ovary cells.
abstract: UVB and UVC toxicity was detected in Chinese Hamster Ovary (CHO) cell lines AA8, UV5 and XEM2 (a V79-derived cell line expressing rat P450 1A1). Unlike FICZ-HPLC assay that showed induction of CYP1A1 enzyme activity after 20 minutes and 2 hour UVC exposure, the EROD assay showed no difference in cytochrome P450 1A1 (CYP1A1) activity after exposure to different doses of UVB and UVC light. Different cytotoxic and mutagenic effect of photo lesions induced by UVC and UVB light was investigated with the DRAG and HPRT assays, comparing the wild type cell line AA8 and the Nucleotide Excision Repair (NER) deficient cell line UV5. DRAG assay showed a significant difference in UV induced cytotoxicity between UVC and UVB reflecting the larger energy and toxic effect of UVC along with significant difference in UV induced toxicity between AA8 and UV5 cell lines. This was further validated through the HPRT assay, which also showed a significant difference in UVC (5 J/m(2)) induced mutagenic effect between these cell lines. In addition, HPRT assay showed the mutagenic effect of photosensitizer, acetophenone. These results show that UVB and UVC generate serious damage through photo products on DNA, and might induce the metabolic activity of CYP1A1.
authors.affiliation: Gandhara College of Pharmacy, Gandhara University, Peshawar, KPK, Pakistan.
authors.forename: Inayatur
authors.initials: I
authors.lastname: Rahman
authors.affiliation: Department of Genetics, Microbiology and Toxicology, Stockholm University, SE Stockholm, Sweden.
authors.forename: Abdul
authors.initials: A
authors.lastname: Karim
authors.affiliation: Department of Pathology, Khyber medical College, Peshawar, KPK, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Idrees
authors.affiliation: Department of Chemistry, Kohat University of Science and Technology, Kohat, KPK, Pakistan.
authors.forename: Mohammad Iqbal
authors.initials: MI
authors.lastname: Khan
mesh_terms: D000818:Animals; D016466:CHO Cells / Q000528:radiation effects*; D002227:Carbazoles / Q000378:metabolism; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D006224:Cricetinae; D003412:Cricetulus; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics / Q000378:metabolism; D004260:DNA Repair; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics / Q000378:metabolism; D009154:Mutation / Q000235:genetics* / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: C111855:6-formylindolo(3,2-b)carbazole; D002227:Carbazoles; D019363:Cytochrome P-450 CYP1A1; D007041:Hypoxanthine Phosphoribosyltransferase