title: Regional distribution of anchorless prion protein, PrP226*, in the human brain.
abstract: It was shown previously that truncated molecules of prion protein can be found in brains of patients with some types of transmissible spongiform encephalopathy. One such molecule, PrP226*, is a fragment of prion protein, truncated at Tyr226. It was found to be present in aggregates, from which it can be released using chaotropic salts. In this study we investigated the distribution of PrP226* in Creutzfeldt-Jakob disease affected human brain, employing the mAb V5B2, specifically recognizing this fragment. The results show that PrP226* is not evenly distributed among different regions of human brain. Among brain regions analyzed, the fragment was found most likely to be accumulated in the cerebellum. Its distribution correlates with the distribution of PrP(Sc).
authors.affiliation: Department for the Production of Diagnostic Reagents and Research; Blood Transfusion Centre of Slovenia; Ljubljana, Slovenia.
authors.forename: Anja
authors.initials: A
authors.lastname: Lukan
authors.affiliation: Department for the Production of Diagnostic Reagents and Research; Blood Transfusion Centre of Slovenia; Ljubljana, Slovenia.
authors.forename: Maja
authors.initials: M
authors.lastname: Černilec
authors.affiliation: Department for the Production of Diagnostic Reagents and Research; Blood Transfusion Centre of Slovenia; Ljubljana, Slovenia.
authors.forename: Tanja
authors.initials: T
authors.lastname: Vranac
authors.affiliation: Institut for Pathology; Medical Faculty; University of Ljubljana; Korytkova, Ljubljana.
authors.forename: Mara
authors.initials: M
authors.lastname: Popović
authors.affiliation: Department for the Production of Diagnostic Reagents and Research; Blood Transfusion Centre of Slovenia; Ljubljana, Slovenia.
authors.forename: Vladka
authors.initials: V
authors.lastname: Čurin Šerbec
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001921:Brain / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011328:Prions / Q000378:metabolism*
chemical_list: D011328:Prions
keywords: PrP fragment; PrP226*; anchorless PrP; denaturation; immunoassay; mAb V5B2; regional distribution