title: Multi-armed poly(L-glutamic acid)-graft-polypropyleneinime as effective and serum resistant gene delivery vectors.
abstract: A new series of multi-armed MP-g-PPI dendrimers were synthesized by polymerization of BLG-NCA using G2.0PPI as macromolecular initiator and subsequent aminolysis with G1.0PPI or G2.0PPI. The chemical structure and composition of the MP-g-PPI dendrimers were characterized by Fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance spectroscopy ((1)H NMR). The MP-g-PPI showed a great ability to combine with pDNA to form complexes, which protect the pDNA from nuclease degradation. Dynamic light scattering (DLS) measurement illustrated that the sizes of complexes were in range of 111-219 nm. The transmission electron microscope (TEM) and atomic force microscope (AFM) observation showed that the morphology of these complexes was spherical. The MTT assay demonstrated that cytotoxicity of the MP-g-PPI was lower than that of PEI 25K. The in vitro transfection test indicated that MP-g-PPI gene vectors displayed relative high transfection efficiency than that of PEI 25K and Lipofectamine 2000 in serum-containing medium. Furthermore, MP-g-PPI at the weight ratio of 7.5 displayed better serum-resistant capability than that of PEI 25K and Lipofectamine 2000. The above facts revealed that multi-armed MP-g-PPI dendrimers may be promising gene vectors with low cytotoxicity, high transfection efficiency and serum-resistant ability.
authors.affiliation: Shenzhen Mental Health Center, 1080 Cuizhu Road, Shenzhen 518020, PR China; Shenzhen Key Lab for Psychological Healthcare, 1080 Cuizhu Road, Shenzhen 518020, PR China. Electronic address: zhangxuan0222@163.com.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Biosciences and Biopharmaceutics, Guang Dong Pharmaceutical University, 280 Waihuan East Road, Guangzhou 510006, PR China.
authors.forename: Haimei
authors.initials: H
authors.lastname: Hu
authors.affiliation: Shenzhen Mental Health Center, 1080 Cuizhu Road, Shenzhen 518020, PR China; Shenzhen Key Lab for Psychological Healthcare, 1080 Cuizhu Road, Shenzhen 518020, PR China.
authors.forename: Tiebang
authors.initials: T
authors.lastname: Liu
authors.affiliation: Shenzhen Mental Health Center, 1080 Cuizhu Road, Shenzhen 518020, PR China.
authors.forename: Yeya
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, 1068 Xueyuan Anenue, Shenzhen University Town, Shenzhen 518055, PR China. Electronic address: gzpshr@sina.com.
authors.forename: Yuefeng
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, 1068 Xueyuan Anenue, Shenzhen University Town, Shenzhen 518055, PR China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Cai
authors.affiliation: Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, 1068 Xueyuan Anenue, Shenzhen University Town, Shenzhen 518055, PR China.
authors.forename: Lijing
authors.initials: L
authors.lastname: Fang
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D050091:Dendrimers / Q000138:chemical synthesis / Q000378:metabolism* / Q000633:toxicity; D003850:Deoxyribonuclease I / Q000378:metabolism; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008027:Light; D008055:Lipids / Q000737:chemistry; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D010316:Particle Size; D066244:Proton Magnetic Resonance Spectroscopy; D012542:Scattering, Radiation; D044967:Serum / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D014162:Transfection / Q000379:methods*
chemical_list: D050091:Dendrimers; D008055:Lipids; C086724:Lipofectamine; D004247:DNA; D003850:Deoxyribonuclease I
keywords: 2-hydroxypyridine (PubChem CID: 8871); DAB-Am-4 (PubChem CID: 4453621); DAB-Am-8 (PubChem CID: 3425467); Ethylenimine (PubChem CID: 9033); Gene vector; MP-g-PPI dendrimer; Poly-gamma-benzyl-l-glutamate (PubChem CID: 95441); Polypropyleneinime; Self-assembly; Transfection efficiency; l-glutamic acid (PubChem CID: 33032)