title: Handheld Diffuse Reflectance Spectral Imaging (DRSi) for in-vivo characterization of skin.
abstract: Diffuse reflectance spectroscopy provides a noninvasive means to measure optical and physiological properties of tissues. To expand on these measurements, we have developed a handheld diffuse reflectance spectral imaging (DRSi) system capable of acquiring wide field hyperspectral images of tissue. The image acquisition time was approximately 50 seconds for a 50x50 pixel image. A transport model was used to fit each spectra for reduced scattering coefficient, hemoglobin concentration and melanin concentration resulting in optical property maps. The system was validated across biologically relevant levels of reduced scattering (5.14% error) and absorption (8.34% error) using tissue simulating phantoms. DRSi optical property maps of a pigmented skin lesion were acquired in vivo. These trends in optical properties were consistent with previous observations using point probe devices.
authors.affiliation: Department of Biomedical Engineering, The University of Texas at Austin, 107. W. Dean Keeton St. Austin TX 78712, USA.
authors.forename: Sheldon F
authors.initials: SF
authors.lastname: Bish
authors.affiliation: Department of Biomedical Engineering, The University of Texas at Austin, 107. W. Dean Keeton St. Austin TX 78712, USA.
authors.forename: Manu
authors.initials: M
authors.lastname: Sharma
authors.affiliation: Department of Biomedical Engineering, The University of Texas at Austin, 107. W. Dean Keeton St. Austin TX 78712, USA.
authors.forename: Youmin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Biomedical Engineering, The University of Texas at Austin, 107. W. Dean Keeton St. Austin TX 78712, USA.
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Triesault
authors.affiliation: Department of Dermatology, University of Texas Medical Branch, 313 E.12th Street, Austin, Texas 78701, USA.
authors.forename: Jason S
authors.initials: JS
authors.lastname: Reichenberg
authors.affiliation: Department of Biomedical Engineering, The University of Texas at Austin, 107. W. Dean Keeton St. Austin TX 78712, USA.
authors.forename: John X J
authors.initials: JX
authors.lastname: Zhang
authors.affiliation: Department of Biomedical Engineering, The University of Texas at Austin, 107. W. Dean Keeton St. Austin TX 78712, USA.
authors.forename: James W
authors.initials: JW
authors.lastname: Tunnell
keywords: (110.0110) Imaging systems; (110.0113) Imaging through turbid media; (170.6510) Spectroscopy, tissue diagnostics