title: Skullcap (Scutellaria baicalensis) extract and its active compound, wogonin, inhibit ovalbumin-induced Th2-mediated response.
abstract: Skullcap (Scutellaria baicalensis) has been widely used as a dietary ingredient and traditional herbal medicine owing to its anti-inflammatory and anticancer properties. In this study, we investigated the anti-allergic effects of skullcap and its active compounds, focusing on T cell-mediated responses ex vivo and in vivo. Splenocytes from mice sensitized with ovalbumin (OVA) were isolated for analyses of cytokine production and cell viability. Mice sensitized with OVA were orally administered skullcap or wogonin for 16 days, and then immunoglobulin (Ig) and cytokine levels were measured by enzyme-linked immunosorbent assays. Treatment with skullcap significantly inhibited interleukin (IL)-4 production without reduction of cell viability. Moreover, wogonin, but not baicalin and baicalein, suppressed IL-4 and interferon-gamma production. In vivo, skullcap and wogonin downregulated OVA-induced Th2 immune responses, especially IgE and IL-5 prediction. Wogonin as an active component of skullcap may be applied as a therapeutic agent for IgE- and IL-5-mediated allergic disorders.
authors.affiliation: Division of Metabolism & Functionality Research, Korea Food Research Institute, 1201-62, Anyangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 463-746, Korea. hsshin@kfri.re.kr.
authors.forename: Hee Soon
authors.initials: HS
authors.lastname: Shin
authors.affiliation: Division of Metabolism & Functionality Research, Korea Food Research Institute, 1201-62, Anyangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 463-746, Korea. mjbae1231@snu.ac.kr.
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Bae
authors.affiliation: Division of Metabolism & Functionality Research, Korea Food Research Institute, 1201-62, Anyangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 463-746, Korea. choidw19@gmail.com.
authors.forename: Dae Woon
authors.initials: DW
authors.lastname: Choi
authors.affiliation: Division of Metabolism & Functionality Research, Korea Food Research Institute, 1201-62, Anyangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 463-746, Korea. dhs95@kfri.re.kr.
authors.forename: Dong-Hwa
authors.initials: DH
authors.lastname: Shon
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000737:chemistry / Q000494:pharmacology; D044950:Flavanones / Q000737:chemistry / Q000494:pharmacology*; D006967:Hypersensitivity / Q000188:drug therapy / Q000276:immunology; D007113:Immunity, Innate / Q000187:drug effects*; D051379:Mice; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D031364:Scutellaria baicalensis / Q000737:chemistry; D018418:Th2 Cells / Q000187:drug effects* / Q000276:immunology
chemical_list: D018926:Anti-Allergic Agents; D044950:Flavanones; D010936:Plant Extracts; C499814:Scutellaria baicalensis extract; D010047:Ovalbumin; C085514:wogonin