title: Matricellular protein Cfl1 regulates cell differentiation.
abstract: Like higher eukaryotic cells in tissues, microbial cells in a community act in concert in response to environmental stimuli. They coordinate gene expression and their physiological and morphological states through intercellular communication mediated by matricellular signals. The adhesion protein Cfl1 was recently shown to be a matricellular signal in regulating morphogenesis and biofilm formation in the eukaryotic microbe Cryptococcus neoformans. Cfl1 is naturally highly expressed in the hyphal subpopulation during the mating colony development. Some Cfl1 proteins are cleaved and released to the ECM (extracellular matrix). The released exogenous Cfl1 activates Cryptococcus cells to express their endogenous Cfl1, to undergo filamentation, and to form structured biofilm colonies. In this study, we demonstrate that the N-terminal signal peptide and the novel conserved cysteine-rich SIGC domain at the C-terminus are critical for the adherence property and the signaling activity of this multifunctional protein. The investigation of this fungal matricellular signaling network involving Cfl1 and the master regulator of morphogenesis Znf2 provides a foundation to further elucidate intercellular communication in microbial development.
authors.affiliation: Department of Biology; Texas A&M University; College Station, TX USA.
authors.forename: Xiuyun
authors.initials: X
authors.lastname: Tian
authors.affiliation: Department of Biology; Texas A&M University; College Station, TX USA.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Lin
keywords: autoinducer; biofilm; filamentation; matricellular signal; paracrine