title: Synthesis, characterization, and evaluation of a novel amphiphilic polymer RGD-PEG-Chol for target drug delivery system.
abstract: An amphiphilic polymer RGD-PEG-Chol which can be produced in large scale at a very low cost has been synthesized successfully. The synthesized intermediates and final products were characterized and confirmed by ¹H nuclear magnetic resonance spectrum (¹H NMR) and Fourier transform infrared spectrum (FT-IR). The paclitaxel- (PTX-) loaded liposomes based on RGD-PEG-Chol were then prepared by film formation method. The liposomes had a size within 100 nm and significantly enhanced the cytotoxicity of paclitaxel to B16F10 cell as demonstrated by MTT test (IC₅₀ = 0.079  μg/mL of RGD-modified PTX-loaded liposomes compared to 9.57  μg/mL of free PTX). Flow cytometry analysis revealed that the cellular uptake of coumarin encapsulated in the RGD-PEG-Chol modified liposome was increased for HUVEC cells. This work provides a reasonable, facile, and economic approach to prepare peptide-modified liposome materials with controllable performances and the obtained linear RGD-modified PTX-loaded liposomes might be attractive as a drug delivery system.
authors.affiliation: State Key Laboratory of Biotherapy and Department of Pharmacy, West China Hospital, Sichuan University, No. 37 Guoxue Alley, Chengdu, Sichuan 610041, China.
authors.forename: Shi
authors.initials: S
authors.lastname: Zeng
authors.affiliation: State Key Laboratory of Biotherapy and Department of Pharmacy, West China Hospital, Sichuan University, No. 37 Guoxue Alley, Chengdu, Sichuan 610041, China.
authors.forename: Fengbo
authors.identifier: 0000-0002-4537-0414
authors.initials: F
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Biotherapy and Department of Pharmacy, West China Hospital, Sichuan University, No. 37 Guoxue Alley, Chengdu, Sichuan 610041, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.affiliation: State Key Laboratory of Biotherapy and Department of Pharmacy, West China Hospital, Sichuan University, No. 37 Guoxue Alley, Chengdu, Sichuan 610041, China.
authors.forename: Xiangrong
authors.initials: X
authors.lastname: Song
authors.affiliation: State Key Laboratory of Biotherapy and Department of Pharmacy, West China Hospital, Sichuan University, No. 37 Guoxue Alley, Chengdu, Sichuan 610041, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Biotherapy and Department of Pharmacy, West China Hospital, Sichuan University, No. 37 Guoxue Alley, Chengdu, Sichuan 610041, China.
authors.forename: Gu
authors.identifier: 0000-0002-1536-8882
authors.initials: G
authors.lastname: He
authors.affiliation: State Key Laboratory Breeding Base of Systematic research, Development and Utilization of Chinese Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Peng
authors.affiliation: State Key Laboratory Breeding Base of Systematic research, Development and Utilization of Chinese Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D002784:Cholesterol / Q000737:chemistry / Q000494:pharmacology; D003374:Coumarins / Q000737:chemistry / Q000494:pharmacology; D016503:Drug Delivery Systems; D004353:Drug Evaluation, Preclinical; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008081:Liposomes; D051379:Mice; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology; D017239:Paclitaxel / Q000737:chemistry / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D003374:Coumarins; D008081:Liposomes; D009842:Oligopeptides; D011092:Polyethylene Glycols; C047981:arginyl-glycyl-aspartic acid; D002784:Cholesterol; C030123:coumarin; D017239:Paclitaxel