title: Non-de-scanned parallel recording two-photon hyperspectral microscopy with high spectral and spatial resolution.
abstract: A two-photon hyperspectral microscope with non-de-scanned geometry and parallel recording scheme was constructed which had a low dwell time, high spectral resolution, and high spatial resolution. The dwell time was effectively decreased to reduce photodamage through parallel recording, while the non-de-scanned geometry led to a considerable reduction in the signal loss and spectrum distortion. Two experiments were performed to show that this system can solve crosstalk issues and spectrally resolve the intrinsic fluorophores in optically-thick tissues without staining and sectioning.
authors.affiliation: Department of Optics and Photonics, National Central University, 300, Jhongda Rd., Jhongli City, Taoyuan County 32001, Taiwan.
authors.forename: Szu-Yu
authors.initials: SY
authors.lastname: Chen
authors.affiliation: Department of Optics and Photonics, National Central University, 300, Jhongda Rd., Jhongli City, Taoyuan County 32001, Taiwan.
authors.forename: Chiao-Sheng
authors.initials: CS
authors.lastname: Lu
authors.affiliation: Department of Optics and Photonics, National Central University, 300, Jhongda Rd., Jhongli City, Taoyuan County 32001, Taiwan.
authors.forename: Chia-Hua
authors.initials: CH
authors.lastname: Yeh
keywords: (110.4234) Multispectral and hyperspectral imaging; (170.2520) Fluorescence microscopy; (180.4315) Nonlinear microscopy; (180.5810) Scanning microscopy