title: Metabolism of bisphenol A by hyper lignin-degrading fungus Phanerochaete sordida YK-624 under non-ligninolytic condition.
abstract: Recently, we reported the conversion of bisphenol A (BPA) to 4-(2-(4-hydroxyphenyl)propan-2-yl)benzene-1,2-diol (hydroxy-BPA) by hyper lignin-degrading fungus Phanerochaete sordida YK-624 under non-ligninolytic condition. In the present study, the metabolism of hydroxy-BPA by P. sordida YK-624 was demonstrated under non-ligninolytic condition. Under these conditions, approximately 66% of hydroxy-BPA was degraded after 7 d of incubation. High-resolution electrospray ionization mass spectra and nuclear magnetic resonance analyses of the metabolites isolated from the culture broth indicated that hydroxy-BPA was metabolized to 4-(2-(4-hydroxyphenyl)propan-2-yl)-2-methoxyphenol (methoxy-BPA) and to 4-(2-(3,4-dimethoxyphenyl)propan-2-yl)phenol (dimethoxy-BPA) by sequential methylation events. These metabolites showed reduced estrogenic activity compared to BPA. These results suggested that the hydroxy BPA is methylated to two low toxic-methylation metabolites.
authors.affiliation: Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
authors.forename: Jianqiao
authors.initials: J
authors.lastname: Wang
authors.affiliation: Graduate School of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
authors.forename: Yuto
authors.initials: Y
authors.lastname: Yamada
authors.affiliation: Graduate School of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Notake
authors.affiliation: Graduate School of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Todoroki
authors.affiliation: Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
authors.forename: Toshinobu
authors.initials: T
authors.lastname: Tokumoto
authors.affiliation: Marine Science Institute, University of Texas at Austin, 750 Channel View Drive, Port Aransas, TX 78373, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Dong
authors.affiliation: Marine Science Institute, University of Texas at Austin, 750 Channel View Drive, Port Aransas, TX 78373, USA.
authors.forename: Peter
authors.initials: P
authors.lastname: Thomas
authors.affiliation: Graduate School of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan. Electronic address: ahhirai@ipc.shizuoka.ac.jp.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Hirai
authors.affiliation: Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; Graduate School of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kawagishi
mesh_terms: D001559:Benzhydryl Compounds / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D002460:Cell Line; D052244:Endocrine Disruptors / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008031:Lignin / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D020075:Phanerochaete / Q000378:metabolism*; D010636:Phenols / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D008031:Lignin; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; Estrogenic activity; Metabolic pathway; Phanerochaete sordida YK-624