title: Embryonic soluble human leukocyte antigen-G as a marker of embryo competency in assisted reproductive technology for Chinese women.
abstract: OBJECTIVE
  To investigate whether embryonic soluble human leukocyte antigen-G (sHLA-G) could be a noninvasive marker for embryo competency in assisted reproductive technology (ART), which is still controversial due to the different detection assays used and the different culture conditions in laboratories.
  
  
  METHODS
  Based on the standardization of IVF procedures and the embryo culture condition, a total of 205 embryo culture supernatants (ESs) from 92 ART cycles were evaluated for sHLA-G contents by chemiluminescent ELISA assay.
  
  
  RESULTS
  sHLA-G presence could be detected in 30.7% of the ESs tested. In the cycles where at least one of the embryos transferred was positive for sHLA-G, 51.9% of patients (27/52) achieved a clinical pregnancy. In cycles where none of the embryos transferred secreted detectable amounts of sHLA-G, the pregnancy rate was only 30.0% (12/40, p < 0.05). The implantation rate in sHLA-G-positive cycles was also significantly higher (31.5%) than that in sHLA-G-negative cycles (14.9%, p < 0.05).
  
  
  CONCLUSIONS
  The results suggested sHLA-G in ESs as a potential marker of embryo competency in ART programs for the Chinese population.
authors.affiliation: Department of Gynecology and Obstetrics, Chinese PLA General Hospital, Beijing; the Assisted Reproductive Center, General Hospital of Guangzhoul Military Command, Guangzhou, China.
authors.forename: Xin-Yu
authors.initials: XY
authors.lastname: Guo
authors.affiliation: Department of Gynecology and Obstetrics, Chinese PLA General Hospital, Beijing; the Assisted Reproductive Center, General Hospital of Guangzhoul Military Command, Guangzhou, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Jiang
authors.affiliation: Department of Gynecology and Obstetrics, Chinese PLA General Hospital, Beijing; the Assisted Reproductive Center, General Hospital of Guangzhoul Military Command, Guangzhou, China.
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Cheng
authors.affiliation: Department of Gynecology and Obstetrics, Chinese PLA General Hospital, Beijing; the Assisted Reproductive Center, General Hospital of Guangzhoul Military Command, Guangzhou, China.
authors.forename: Cai-Ying
authors.initials: CY
authors.lastname: Hou
authors.affiliation: Department of Gynecology and Obstetrics, Chinese PLA General Hospital, Beijing; the Assisted Reproductive Center, General Hospital of Guangzhoul Military Command, Guangzhou, China.
authors.forename: Yuan-Qing
authors.initials: YQ
authors.lastname: Yao
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D002681:China; D017077:Culture Media, Conditioned / Q000737:chemistry; D046149:Embryo Culture Techniques; D010064:Embryo Implantation; D004622:Embryo, Mammalian / Q000737:chemistry* / Q000276:immunology / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro; D059951:HLA-G Antigens / Q000032:analysis*; D006801:Humans; D011247:Pregnancy; D018873:Pregnancy Rate; D027724:Reproductive Techniques, Assisted; D012995:Solubility
chemical_list: D015415:Biomarkers; D017077:Culture Media, Conditioned; D059951:HLA-G Antigens