title: [Construction of recombinant CHO cell strain for high expression of HBsAg].
abstract: OBJECTIVE
  To overexpress hepatitis B virus S gene in CHO cells cultured in serum-free media.
  
  
  METHODS
  Plasmid was constructed by cloning of HBV S gene and then it was transfected into CHO cells. After cell screen, the positive clones were identified and isolated into a serum-free media followed by the serological and morphological characterization of the expression product.
  
  
  RESULTS
  CHO cell strains which can express HBsAg efficiently and stably were obtained. Spherical and filamentous HBsAg could be detected under electronic microscope. The titer of the expression product was up to 1:5000.
  
  
  CONCLUSIONS
  Serum-free media cultured CHO cell strain for overexpression of HBsAg was successfully constructed and the expression product was high antigenic.
authors.affiliation: National Institute for Viral Disease Contral and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Yi
authors.affiliation: National Institute for Viral Disease Contral and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Min-Zhuo
authors.initials: MZ
authors.lastname: Guo
authors.affiliation: National Institute for Viral Disease Contral and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Rui-Guang
authors.initials: RG
authors.lastname: Tian
authors.affiliation: National Institute for Viral Disease Contral and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Qiu-Dong
authors.initials: QD
authors.lastname: Su
authors.affiliation: National Institute for Viral Disease Contral and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Xue-Xin
authors.initials: XX
authors.lastname: Lu
authors.affiliation: National Institute for Viral Disease Contral and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Qiu
authors.affiliation: National Institute for Viral Disease Contral and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Wen-Ting
authors.initials: WT
authors.lastname: Zhou
authors.affiliation: National Institute for Viral Disease Contral and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Zhi-Yuan
authors.initials: ZY
authors.lastname: Jia
authors.affiliation: National Institute for Viral Disease Contral and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Sheng-Li
authors.initials: SL
authors.lastname: Bi
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D015870:Gene Expression; D006514:Hepatitis B Surface Antigens / Q000235:genetics* / Q000276:immunology; D014162:Transfection
chemical_list: D006514:Hepatitis B Surface Antigens