title: Characterization of a β-1,4-mannanase from a newly isolated strain of Pholiota adiposa and its application for biomass pretreatment.
abstract: A highly efficient β-1,4-mannanase-secreting strain, Pholiota adiposa SKU0714, was isolated and identified on the basis of its morphological features and sequence analysis of internal transcribed spacer rDNA. P. adiposa β-1,4-mannanase was purified to homogeneity from P. adiposa culture supernatants by one-step chromatography on a Sephacryl gel filtration column. P. adiposa β-1,4-mannanase showed the highest activity toward locust bean gum (V max = 1,990 U/mg protein, K m = 0.12 mg/mL) ever reported. Its internal amino acid sequence showed homology with hydrolases from the glycoside hydrolase family 5 (GH5), indicating that the enzyme is a member of the GH5 family. The saccharification of commercial mannanase and P. adiposa β-1,4-mannanase-pretreated rice straw by Celluclast 1.5L (Novozymes) was compared. In comparison with the commercial Novo Mannaway(®) (113 mg/g-substrate), P. adiposa β-1,4-mannanase-pretreated rice straw released more reducing sugars (141 mg/g-substrate). These properties make P. adiposa β-1,4-mannanase a good candidate as a new commercial β-1,4-mannanase to improve biomass pretreatment.
authors.affiliation: Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul, 143-701, Republic of Korea.
authors.forename: Priyadharshini
authors.initials: P
authors.lastname: Ramachandran
authors.forename: Zongpei
authors.initials: Z
authors.lastname: Zhao
authors.forename: Raushan
authors.initials: R
authors.lastname: Singh
authors.forename: Saurabh Sudha
authors.initials: SS
authors.lastname: Dhiman
authors.forename: Joon-Ho
authors.initials: JH
authors.lastname: Choi
authors.forename: Dongwook
authors.initials: D
authors.lastname: Kim
authors.forename: Jung-Rim
authors.initials: JR
authors.lastname: Haw
authors.forename: Jung-Kul
authors.initials: JK
authors.lastname: Lee
mesh_terms: D001483:Base Sequence; D018533:Biomass; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D055368:Pholiota / Q000201:enzymology*; D013696:Temperature; D044902:beta-Mannosidase / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D044902:beta-Mannosidase