title: Id1 and NF-κB promote the generation of CD133+ and BMI-1+ keratinocytes and the growth of xenograft tumors in mice.
abstract: Id1 and NF-κB are highly expressed in oral squamous cell carcinoma (OSCC). Whether they have a synergistic role in the carcinogenesis of OSCC is unclear. The current study was designed to demonstrate the synergy of both Id1 and NF-κB in the underlying disease mechanisms of OSCC using in vitro and in vivo animal models. Id1 and NF-κB strengthened the expression of both CD133 and BMI-1 in OSCC cell cultures. CD133(+) and BMI-1(+) keratinocytes from OSCC tissues and cell cultures initiated the growth of xenograft tumors in SCID/Beige mice. Id1 and NF-κB regulate the expression of CD133 and BMI-1 in an additive or synergistic manner in OSCC, which is associated with the generation of naïve and self-renewable keratinocytes and initiate the growth of xenograft tumors in vivo.
authors.affiliation: Department of Oncology of Union Hospital, Institute of Immunotherapy, Fujian Medical University, Fuzhou, P.R. China.
authors.forename: Jinhuo
authors.initials: J
authors.lastname: Lai
authors.affiliation: Department of Otolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangdong, P.R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Cai
authors.affiliation: Ear, Nose and Throat Specialty Care of Minnesota, Minneapolis, MN, USA.
authors.forename: Merrill A
authors.initials: MA
authors.lastname: Biel
authors.affiliation: Department of Oncology of Union Hospital, Institute of Immunotherapy, Fujian Medical University, Fuzhou, P.R. China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Oncology of Union Hospital, Institute of Immunotherapy, Fujian Medical University, Fuzhou, P.R. China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Hu
authors.affiliation: Department of Oncology of Union Hospital, Institute of Immunotherapy, Fujian Medical University, Fuzhou, P.R. China.
authors.forename: Shaoyuan
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Oncology of Union Hospital, Institute of Immunotherapy, Fujian Medical University, Fuzhou, P.R. China.
authors.forename: Jizhen
authors.initials: J
authors.lastname: Lin
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006023:Glycoproteins / Q000378:metabolism*; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000473:pathology; D064593:Heterografts / Q000473:pathology; D006801:Humans; D051798:Inhibitor of Differentiation Protein 1 / Q000235:genetics / Q000378:metabolism*; D015603:Keratinocytes / Q000378:metabolism*; D051379:Mice; D016513:Mice, SCID; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D009368:Neoplasm Transplantation / Q000473:pathology; D010455:Peptides / Q000378:metabolism*; D063150:Polycomb Repressive Complex 1 / Q000378:metabolism*; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism*
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; D051798:Inhibitor of Differentiation Protein 1; C000606023:PROM1 protein, human; D010455:Peptides; C000606024:Prom1 protein, mouse; D051996:Transcription Factor RelA; D063150:Polycomb Repressive Complex 1