title: Alleviation of methyl viologen-mediated oxidative stress by Brassica juncea annexin-3 in transgenic Arabidopsis.
abstract: Plant annexins function as calcium-dependent or -independent phospholipid binding proteins and constitute about 0.1% of total cellular proteins. Some of them were reported to antagonize oxidative stress and protect plant cells. Brassica juncea annexin-3 (AnnBj3) was recently discovered. To gain insight into a possible function of AnnBj3 in oxidative stress response, we investigated the resistance of Arabidopsis thaliana plants expressing AnnBj3 constitutively. Here we report that, AnnBj3 attenuates methyl viologen-mediated oxidative stress in plants. It protected photosynthesis and plasma membrane from methyl viologen-mediated oxidative damage. AnnBj3 detoxifies hydrogen peroxide and showed antioxidative property in vitro. The protein increased total peroxidase activity in transgenics and interfered with other cellular antioxidants, thereby giving an overall cellular protection against methyl viologen-induced cytotoxicity.
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India. Electronic address: ahan.dalal@daad-alumni.de.
authors.forename: Ahan
authors.initials: A
authors.lastname: Dalal
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India.
authors.forename: Abhay
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India.
authors.forename: Deepanker
authors.initials: D
authors.lastname: Yadav
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India.
authors.forename: Triveni
authors.initials: T
authors.lastname: Gudla
authors.affiliation: Department of Plant Biochemistry and Physiology, Faculty of Biology, University of Bielefeld, Bielefeld, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Viehhauser
authors.affiliation: Department of Plant Biochemistry and Physiology, Faculty of Biology, University of Bielefeld, Bielefeld, Germany.
authors.forename: Karl-Josef
authors.initials: KJ
authors.lastname: Dietz
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India.
authors.forename: Pulugurtha Bharadwaja
authors.initials: PB
authors.lastname: Kirti
mesh_terms: D017318:Annexin A3 / Q000502:physiology*; D000975:Antioxidants / Q000378:metabolism; D017360:Arabidopsis / Q000502:physiology*; D006706:Homeostasis; D009149:Mustard Plant / Q000235:genetics*; D018384:Oxidative Stress; D010269:Paraquat; D009195:Peroxidase / Q000378:metabolism; D045332:Photosystem II Protein Complex / Q000378:metabolism; D010940:Plant Proteins / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D009195:Peroxidase; D017318:Annexin A3; D010269:Paraquat
keywords: Antioxidative property; Brassica juncea annexin-3; Membrane integrity; Methyl viologen; Photosynthetic performance; Redox buffer