title: Conducting polymer electrodes for gel electrophoresis.
abstract: In nearly all cases, electrophoresis in gels is driven via the electrolysis of water at the electrodes, where the process consumes water and produces electrochemical by-products. We have previously demonstrated that π-conjugated polymers such as poly(3,4-ethylenedioxythiophene) (PEDOT) can be placed between traditional metal electrodes and an electrolyte to mitigate electrolysis in liquid (capillary electroosmosis/electrophoresis) systems. In this report, we extend our previous result to gel electrophoresis, and show that electrodes containing PEDOT can be used with a commercial polyacrylamide gel electrophoresis system with minimal impact to the resulting gel image or the ionic transport measured during a separation.
authors.affiliation: Transport and Separations Group, Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden.
authors.forename: Katarina
authors.initials: K
authors.lastname: Bengtsson
authors.affiliation: Transport and Separations Group, Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden.
authors.forename: Sara
authors.initials: S
authors.lastname: Nilsson
authors.affiliation: Transport and Separations Group, Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden.
authors.forename: Nathaniel D
authors.initials: ND
authors.lastname: Robinson
mesh_terms: D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation* / Q000379:methods*; D011108:Polymers / Q000737:chemistry*
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D011108:Polymers; C121383:poly(3,4-ethylene dioxythiophene)