title: [Study on establishment of mass spectrometry database of salmonella species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry].
abstract: OBJECTIVE
  To establish the mass spectrometry database of Salmonella species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
  
  
  METHODS
  By use of standard stains representing 21 species of Salmonella, a reference database was established MALDI-TOF-MS-based species identification. The profiles of colonies in the identical culture condition with different times were analysed for each of standard strains. To evaluate the database, 200 blind-coded isolates were tested by the established database and the original database.
  
  
  RESULTS
  The positive rate of identification by the original database, the established database and conbine-database were 87.5%, 97.0% and 97.5%. 21 Salmonella strains were divided into 3 types by MALDI-TOF-MS at distance level of 500. PCA of the reference strains showed difference between them. There were little changes of mass spectrometric data of several strains in different culture condition.
  
  
  CONCLUSIONS
  The established database could expand the original one and be used for identification of Salmonella species. The strategy may be fit for other bacteria.
authors.affiliation: Logistics University of Chinese People's Armed Police Force, Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazard, Tianjin 300162, China. huxiao0711@163.com
authors.forename: Xiao
authors.initials: X
authors.lastname: Hu
authors.affiliation: Logistics University of Chinese People's Armed Police Force, Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazard, Tianjin 300162, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Lin
authors.affiliation: Logistics University of Chinese People's Armed Police Force, Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazard, Tianjin 300162, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Tong
authors.affiliation: Logistics University of Chinese People's Armed Police Force, Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazard, Tianjin 300162, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Logistics University of Chinese People's Armed Police Force, Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazard, Tianjin 300162, China.
authors.forename: Guohui
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Logistics University of Chinese People's Armed Police Force, Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazard, Tianjin 300162, China.
authors.forename: Huabing
authors.initials: H
authors.lastname: Zhao
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D016208:Databases, Factual; D012475:Salmonella / Q000145:classification* / Q000302:isolation & purification*; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*