title: Poly(A) polymerase-based poly(A) length assay.
abstract: mRNA polyadenylation functions in nuclear export, translation, and stability. We describe an efficient protocol designed to assess poly(A) tail length that is based on 3' tailing by yeast poly(A) polymerase and product analysis to single-nucleotide resolution by capillary electrophoresis.
authors.affiliation: Center for RNA Biology, The Ohio State University, Columbus, OH, USA.
authors.forename: Deepak P
authors.initials: DP
authors.lastname: Patil
authors.forename: Baskar
authors.initials: B
authors.lastname: Bakthavachalu
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Schoenberg
mesh_terms: D019075:Electrophoresis, Capillary; D005821:Genetic Techniques; D011061:Poly A / Q000378:metabolism*; D011062:Polynucleotide Adenylyltransferase / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D011061:Poly A; D011062:Polynucleotide Adenylyltransferase