title: Epidemiology of toxoplasmosis in white tailed deer (Odocoileus virginianus): occurrence, congenital transmission, correlates of infection, isolation, and genetic characterization of Toxoplasma gondii.
abstract: The prevalence of Toxoplasma gondii in white tailed deer (WTD) in the USA is high but little is known of the epidemiology of toxoplasmosis in this host. In the present study, we compared T. gondii seroprevalence from 749 WTD collected in 2012 and 2013 from a Metropolitan Park in Ohio and 487 WTD deer shot in Minnesota during 2008, 2009, and 2010. Serum samples were tested for antibodies to T. gondii by the modified agglutination test (cut-off titer, 25). Additionally myocardial samples from 123 seropositive WTD from Ohio were digested in pepsin and the digests were bioassayed for the isolation of T. gondii. Furthermore, to estimate transplacental rate of transmission, brains from 155 fetuses (included twins) from 148 deer from Minnesota were bioassayed in mice for the isolation of viable T. gondii. Seroprevalence of T. gondii varied with the year of collection, geography, and the age of deer. Of the Ohio deer sampled in 2012 and 2013 seroprevalences for the two years were similar (73.4% and 75.7%, respectively); remarkably 150 (66.1%) of 227 deer of <1 year of age were seropositive. Of the Minnesota deer, seroprevalence was lowest for the year 2008 (14.8%, 26/175) versus 2009 (27.7%, 59/213), and 2010 (25.2%, 25/99), thought to be related to environmental temperatures. Viable T. gondii was isolated in mice from the myocardium of four WTD from Ohio, and brain of one WTD fetus from Minnesota. Tachyzoites from infected mouse tissues were further propagated in cell culture. The DNA isolated from culture-derived tachyzoites of these five T. gondii isolates was characterized using 11 PCR-RFLP markers (SAG1, 5'- and 3'-SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico). Four genotypes were found, including ToxoDB genotype no. 1 (Type II), no. 2 (Type III), no. 3 (Type II variant) and no. 146. Results indicate fluctuating seroprevalence, probably related to weather and warrant further epidemiological studies.
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705-2350, USA. Electronic address: Jitender.dubey@ars.usda.gov.
authors.forename: J P
authors.initials: JP
authors.lastname: Dubey
authors.affiliation: Cleveland Metroparks Zoo, 3900 Wildlife Way, Cleveland, OH 44109, USA.
authors.forename: P M
authors.initials: PM
authors.lastname: Dennis
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705-2350, USA.
authors.forename: S K
authors.initials: SK
authors.lastname: Verma
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705-2350, USA.
authors.forename: S
authors.initials: S
authors.lastname: Choudhary
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705-2350, USA.
authors.forename: L R
authors.initials: LR
authors.lastname: Ferreira
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705-2350, USA.
authors.forename: S
authors.initials: S
authors.lastname: Oliveira
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705-2350, USA.
authors.forename: O C H
authors.initials: OC
authors.lastname: Kwok
authors.affiliation: Division of Wildlife, Minnesota Department of Natural Resources, 5463-C West Broadway, Forest Lake, MN 55025, USA.
authors.forename: E
authors.initials: E
authors.lastname: Butler
authors.affiliation: Division of Wildlife, Minnesota Department of Natural Resources, 5463-C West Broadway, Forest Lake, MN 55025, USA.
authors.forename: M
authors.initials: M
authors.lastname: Carstensen
authors.affiliation: Department of Microbiology, The University of Tennessee, Knoxville, TN 37996-0845, USA.
authors.forename: C
authors.initials: C
authors.lastname: Su
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D016054:DNA, Protozoan / Q000235:genetics*; D003670:Deer / Q000469:parasitology*; D005260:Female; D005838:Genotype; D008297:Male; D051379:Mice; D008910:Minnesota / Q000453:epidemiology; D009820:Ohio / Q000453:epidemiology; D016036:Seroepidemiologic Studies; D014122:Toxoplasma / Q000235:genetics* / Q000302:isolation & purification; D014124:Toxoplasmosis, Animal / Q000453:epidemiology* / Q000635:transmission
chemical_list: D000913:Antibodies, Protozoan; D016054:DNA, Protozoan
keywords: Epidemiology; Isolation; Serology; Toxoplasma gondii; White tailed deer