title: Immunophotoaffinity labeling of the binding proteins for 1-methyladenine, an oocyte maturation-inducing hormone of starfish.
abstract: Starfish oocytes are naturally arrested at the prophase stage of the first meiotic division and resume meiosis in response to 1-methyladenine (1-MeAde), the oocyte maturation-inducing hormone of starfish. Putative receptors for 1-MeAde have not yet been characterized biochemically, although the specific binding of 1-MeAde to the isolated cortices of starfish oocytes was reported so far. Based on the structure-activity relationship of 1-MeAde analogs, we have designed a photoaffinity labeling reagent. The photoaffinity labeling of oocyte membrane fractions, followed by immunoblotting analysis with anti-1-MeAde antibody, results in the detection of an almost single protein band. This 1-MeAde-binding protein might be a possible candidate of the maturation-inducing hormone receptor of starfish.
authors.affiliation: Graduate School of Science and Technology, Okayama University, Okayama, Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Kida
authors.forename: Shinjiro
authors.initials: S
authors.lastname: Matsuda
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kuyama
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Toraya
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000737:chemistry; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism*; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D009865:Oocytes / Q000378:metabolism*; D019920:Photoaffinity Labels / Q000737:chemistry*; D011817:Rabbits; D013194:Staining and Labeling; D013215:Starfish / Q000166:cytology / Q000378:metabolism*
chemical_list: D000906:Antibodies; D002352:Carrier Proteins; D019920:Photoaffinity Labels; C008407:1-methyladenine; D000225:Adenine