title: Arginine methylation of CRTC2 is critical in the transcriptional control of hepatic glucose metabolism.
abstract: Fasting glucose homeostasis is maintained in part through cAMP (adenosine 3',5'-monophosphate)-dependent transcriptional control of hepatic gluconeogenesis by the transcription factor CREB (cAMP response element-binding protein) and its coactivator CRTC2 (CREB-regulated transcriptional coactivator 2). We showed that PRMT6 (protein arginine methyltransferase 6) promotes fasting-induced transcriptional activation of the gluconeogenic program involving CRTC2. Mass spectrometric analysis indicated that PRMT6 associated with CRTC2. In cells, PRMT6 mediated asymmetric dimethylation of multiple arginine residues of CRTC2, which enhanced the association of CRTC2 with CREB on the promoters of gluconeogenic enzyme-encoding genes. In mice, ectopic expression of PRMT6 promoted higher blood glucose concentrations, which were associated with increased expression of genes encoding gluconeogenic factors, whereas knockdown of hepatic PRMT6 decreased fasting glycemia and improved pyruvate tolerance. The abundance of hepatic PRMT6 was increased in mouse models of obesity and insulin resistance, and adenovirus-mediated depletion of PRMT6 restored euglycemia in these mice. We propose that PRMT6 is involved in the regulation of hepatic glucose metabolism in a CRTC2-dependent manner.
authors.affiliation: 1Division of Life Sciences, Korea University, 145 Anam-Ro, Seongbuk-Gu, Seoul 136-713, Korea.
authors.forename: Hye-Sook
authors.initials: HS
authors.lastname: Han
authors.forename: Chang-Yun
authors.initials: CY
authors.lastname: Jung
authors.forename: Young-Sil
authors.initials: YS
authors.lastname: Yoon
authors.forename: Seri
authors.initials: S
authors.lastname: Choi
authors.forename: Dahee
authors.initials: D
authors.lastname: Choi
authors.forename: Geon
authors.initials: G
authors.lastname: Kang
authors.forename: Keun-Gyu
authors.initials: KG
authors.lastname: Park
authors.forename: Seong-Tae
authors.initials: ST
authors.lastname: Kim
authors.forename: Seung-Hoi
authors.initials: SH
authors.lastname: Koo
mesh_terms: D000818:Animals; D001120:Arginine / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D000242:Cyclic AMP / Q000235:genetics / Q000378:metabolism; D005943:Gluconeogenesis; D005947:Glucose / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007333:Insulin Resistance; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008745:Methylation; D051379:Mice; D009765:Obesity / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D011484:Protein-Arginine N-Methyltransferases / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: C504923:Crtc2 protein, mouse; D014157:Transcription Factors; D001120:Arginine; D000242:Cyclic AMP; C577312:PRMT6 protein, mouse; D011484:Protein-Arginine N-Methyltransferases; D005947:Glucose