title: Long-term use of probiotics Lactobacillus and Bifidobacterium has a prophylactic effect on the occurrence and severity of pouchitis: a randomized prospective study.
abstract: OBJECTIVE
  The aim of the study was to assess the impact of the long-term use of the composite probiotics in patients after restorative proctocolectomy.
  
  
  METHODS
  Forty-three patients (20 females and 23 males, aged 21 to 68 years) after restorative proctocolectomy were included in the study. After randomization patients were divided into placebo group and treatment group with oral intake of probiotic containing Lactobacillus acidophilus, Lactobacillus delbrueckii subsp. bulgaricus, and Bifidobacterium bifidus. Patients were investigated during initial visit and during final visit after 9 months. All patients were subjected to standard clinical and endoscopic examination with microscopic study of the specimens. Concentrations of calprotectin and pyruvate kinase isoenzyme M2-PK were determined in all cases.
  
  
  RESULTS
  The average severity of pouchitis and the number of patients with pouchitis significantly decrease after 9 months of the probiotic taking. The concentrations of calprotectin and pyruvate kinase isoenzyme M2-PK significantly decreased after the therapy.
  
  
  CONCLUSIONS
  Nine months of the probiotic treatment (Lactobacillus acidophilus, Lactobacillus delbrueckii subsp. bulgaricus, and Bifidobacterium bifidus) reduced the number of patients with pouchitis, decreased the PDAI score, and also decreased the fecal pyruvate kinase and calprotectin. The long-term probiotics use is safe and well accepted and can be an effective method of the pouchitis prevention.
authors.affiliation: Department of General Surgery, Oncologic Gastroenterologic Surgery and Plastic Surgery, Poznan University of Medical Sciences, Ulica Przybyszewskiego 49, 60-355 Poznań, Poland.
authors.forename: Banasiewicz
authors.initials: B
authors.lastname: Tomasz
authors.affiliation: Regional Specialist Hospital, Department of General, Vascular and Oncologic Surgery, 76-200 Slupsk, Poland ; Department of Oncologic Surgery, Gdansk Medical University, 80-210 Gdansk, Poland.
authors.forename: Stojcev
authors.initials: S
authors.lastname: Zoran
authors.affiliation: I Chair of Pediatrics, Department of Gastroenterology and Metabolism, Poznan University of Medical Sciences, 60-572 Poznań, Poland.
authors.forename: Walkowiak
authors.initials: W
authors.lastname: Jarosław
authors.affiliation: Department of General Surgery, Oncologic Gastroenterologic Surgery and Plastic Surgery, Poznan University of Medical Sciences, Ulica Przybyszewskiego 49, 60-355 Poznań, Poland.
authors.forename: Marciniak
authors.initials: M
authors.lastname: Ryszard
authors.affiliation: Department of General and Colorectal Surgery, Poznan University of Medical Sciences, 61-285 Poznań, Poland.
authors.forename: Grochowalski
authors.initials: G
authors.lastname: Marcin
authors.affiliation: Department of General and Colorectal Surgery, Poznan University of Medical Sciences, 61-285 Poznań, Poland.
authors.forename: Burdyński
authors.initials: B
authors.lastname: Robert
authors.affiliation: Department of General and Colorectal Surgery, Poznan University of Medical Sciences, 61-285 Poznań, Poland.
authors.forename: Krokowicz
authors.initials: K
authors.lastname: Piotr
authors.affiliation: Department of General Surgery, Oncologic Gastroenterologic Surgery and Plastic Surgery, Poznan University of Medical Sciences, Ulica Przybyszewskiego 49, 60-355 Poznań, Poland.
authors.forename: Krokowicz
authors.initials: K
authors.lastname: Lukasz
authors.affiliation: Department of General Surgery, Oncologic Gastroenterologic Surgery and Plastic Surgery, Poznan University of Medical Sciences, Ulica Przybyszewskiego 49, 60-355 Poznań, Poland.
authors.forename: Paszkowski
authors.initials: P
authors.lastname: Jacek
authors.affiliation: Department of Physiology, University School of Physical Education, 61-871 Poznań, Poland.
authors.forename: Gronek
authors.initials: G
authors.lastname: Piotr
authors.affiliation: Department of General Surgery, Oncologic Gastroenterologic Surgery and Plastic Surgery, Poznan University of Medical Sciences, Ulica Przybyszewskiego 49, 60-355 Poznań, Poland.
authors.forename: Pyda
authors.initials: P
authors.lastname: Przemysław
authors.affiliation: Department of General Surgery, Oncologic Gastroenterologic Surgery and Plastic Surgery, Poznan University of Medical Sciences, Ulica Przybyszewskiego 49, 60-355 Poznań, Poland.
authors.forename: Drews
authors.initials: D
authors.lastname: Michał
mesh_terms: D000328:Adult; D000368:Aged; D001644:Bifidobacterium / Q000502:physiology*; D005260:Female; D006801:Humans; D007778:Lactobacillus / Q000502:physiology*; D039841:Leukocyte L1 Antigen Complex / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010919:Placebos; D019449:Pouchitis / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control*; D019936:Probiotics / Q000627:therapeutic use*; D011446:Prospective Studies; D011770:Pyruvate Kinase / Q000378:metabolism; D012720:Severity of Illness Index; D013997:Time Factors; D055815:Young Adult
chemical_list: D039841:Leukocyte L1 Antigen Complex; D010919:Placebos; D011770:Pyruvate Kinase