title: Stomach microbiota composition varies between patients with non-atrophic gastritis and patients with intestinal type of gastric cancer.
abstract: We aimed to characterize microbiota of the gastric mucosa as it progress to intestinal type of cancer. Study included five patients each of non-atrophic gastritis (NAG), intestinal metaplasia (IM) and intestinal-type gastric cancer (GC). Gastric tissue was obtained and DNA extracted for microbiota analyses using the microarray G3 PhyloChip. Bacterial diversity ranged from 8 to 57, and steadily decreased from NAG to IM to GC (p = 0.004). A significant microbiota difference was observed between NAG and GC based on Unifrac-presence/absence and weighted-Unifrac-abundance metrics of 283 taxa (p < 0.05). HC-AN analyses based on presence/absence of 238 taxa revealed that GC and NAG grouped apart, whereas IM overlapped with both. An ordinated analyses based on weighted-Unifrac distance given abundance of 44 taxa showing significance across categories revealed significant microbiota separation between NAG and GC. This study is the first to show a gradual shift in gastric microbiota profile from NAG to IM to GC.
authors.affiliation: Unidad de Investigación en Enfermedades Infecciosas, Tubo Digestivo Alto, UMAE Oncologia.
authors.forename: Francisco
authors.initials: F
authors.lastname: Aviles-Jimenez
authors.affiliation: Unidad de Investigación en Enfermedades Infecciosas, Tubo Digestivo Alto, UMAE Oncologia.
authors.forename: Flor
authors.initials: F
authors.lastname: Vazquez-Jimenez
authors.affiliation: Departamento de Sarcomas, Tubo Digestivo Alto, UMAE Oncologia.
authors.forename: Rafael
authors.initials: R
authors.lastname: Medrano-Guzman
authors.affiliation: Servicio de Patologia, UMAE Oncologia, Centro Medico Nacional SXXI, Mexico City.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Mantilla
authors.affiliation: Unidad de Investigación en Enfermedades Infecciosas, Tubo Digestivo Alto, UMAE Oncologia.
authors.forename: Javier
authors.initials: J
authors.lastname: Torres
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000382:microbiology*; D005260:Female; D005756:Gastritis / Q000382:microbiology*; D006801:Humans; D007414:Intestinal Neoplasms / Q000382:microbiology*; D008297:Male; D064307:Microbiota; D013270:Stomach / Q000382:microbiology*; D013274:Stomach Neoplasms / Q000382:microbiology*