title: Synchronous alteration pattern between serine-threonine kinase receptor-associated protein and Smad7 in pilocarpine-induced rats of epilepsy.
abstract: OBJECTIVE
  Recent studies have shown that transforming growth factor β (TGFβ) signaling participates in the epileptogenesis. Serine-threonine kinase receptor-associated protein (STRAP) and Smad7 synergize in the inhibition of the TGFβ signaling. The aim of the present study was to determine the expression pattern of STRAP and Smad7 in the hippocampus and temporal lobe cortex of pilocarpine-induced rats models of epilepsy.
  
  
  METHODS
  Lithium chloride-pilocarpine-induced rats with status epilepticus (SE) were established. Total of 60 male Sprague-Dawley rats was used as control (n = 10), 24 h (n = 10), 72 h (n = 10), 1 week (n = 10), 1 month (n = 10), and 2 months (n = 10) after pilocarpine-induced SE, respectively. We detected the expression levels of STRAP and Smad7 in the hippocampus and temporal lobe cortex of rats at the aforementioned time points using western blotting and immunohistochemistry.
  
  
  RESULTS
  STRAP level was significantly decreased in 24 h, 72 h (acute stage), 1 week (latent stage), 1 month, 2 months (chronic stage), respectively, in the rat models compared with the control rats by using both western blotting and immunohistochemistry. Smad7 had similar reduced pattern as STRAP.
  
  
  CONCLUSIONS
  Our results indicate that STRAP and Smad7 proteins might be involved in the development of temporal lobe epilepsy.
authors.affiliation: Institute of Neuroscience, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Rui
authors.initials: R
authors.lastname: Shi
authors.forename: Wenxiu
authors.initials: W
authors.lastname: Yang
authors.forename: Nan
authors.initials: N
authors.lastname: Zhao
authors.forename: Yingshi
authors.initials: Y
authors.lastname: Du
authors.forename: Yan
authors.initials: Y
authors.lastname: Zou
authors.forename: Weihua
authors.initials: W
authors.lastname: Yu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D015153:Blotting, Western; D002908:Chronic Disease; D004195:Disease Models, Animal; D004833:Epilepsy, Temporal Lobe; D015870:Gene Expression; D006624:Hippocampus / Q000378:metabolism*; D007150:Immunohistochemistry; D018021:Lithium Chloride; D008297:Male; D010862:Pilocarpine; D051381:Rats; D017207:Rats, Sprague-Dawley; D051906:Smad7 Protein / Q000378:metabolism*; D013226:Status Epilepticus / Q000378:metabolism*; D013702:Temporal Lobe / Q000378:metabolism*; D013997:Time Factors
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C000593174:STRAP protein, rat; D051906:Smad7 Protein; C497808:Smad7 protein, rat; D010862:Pilocarpine; D018021:Lithium Chloride
keywords: Serine-threonine kinase receptor-associated protein; Smad7; epilepsy; pilocarpine-induced rat model; transforming growth factor β signalling