title: Passive protection of mice pups through oral or intranasal immunization of dams with recombinant Lactobacillus casei vaccine against ETEC F41.
abstract: Mucosal immunization is advantageous over other routes of antigen delivery because it can induce both mucosal and systemic immune responses. In this study, we have developed fimbriae protein of enterotoxigenic Escherichia coli (ETEC) F41 was stably expressed on the surface Lactobacillus casei 525. The method of expressing vaccine antigens in L. casei induces both systemic and mucosal immunity after oral or intranasal administration. We demonstrate that an oral or intranasal vaccine based on live recombinant L. casei 525 protects infant mice from ETEC F41 infection. This platform technology can be applied to design oral or intranasal vaccine delivery vehicles against several microbial pathogens.
authors.affiliation: College of Life Science and Technology, Heilongjiang August First Land Reclamation University, Daqing, Heilongjiang Province 163319, China; College of Life Sciences of Longyan University, Key Laboratory of Preventive Veterinary Medicine and Biotechnology, Longyan, Fujian Province 364012, China. Electronic address: liujiankui99@126.com.
authors.forename: Jian-Kui
authors.initials: JK
authors.lastname: Liu
authors.affiliation: College of Life Science and Technology, Heilongjiang August First Land Reclamation University, Daqing, Heilongjiang Province 163319, China; College of Life Sciences of Longyan University, Key Laboratory of Preventive Veterinary Medicine and Biotechnology, Longyan, Fujian Province 364012, China.
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Wei
authors.affiliation: College of Animal Science and Technology, Heilongjiang August First Land Reclamation University, Daqing, Heilongjiang Province 163319, China.
authors.forename: Xi-Lin
authors.initials: XL
authors.lastname: Hou
authors.affiliation: College of Life Science and Technology, Heilongjiang August First Land Reclamation University, Daqing, Heilongjiang Province 163319, China; College of Animal Science and Technology, Heilongjiang August First Land Reclamation University, Daqing, Heilongjiang Province 163319, China. Electronic address: xly_hou@126.com.
authors.forename: Li-Yun
authors.initials: LY
authors.lastname: Yu
mesh_terms: D000281:Administration, Intranasal; D000284:Administration, Oral; D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western; D054307:Enterotoxigenic Escherichia coli / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D004927:Escherichia coli Infections / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D022361:Escherichia coli Vaccines / Q000008:administration & dosage / Q000276:immunology*; D005260:Female; D010861:Fimbriae, Bacterial / Q000276:immunology*; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation; D013047:Specific Pathogen-Free Organisms; D014611:Vaccination / Q000379:methods* / Q000592:standards
chemical_list: D022361:Escherichia coli Vaccines; D007070:Immunoglobulin A; D007074:Immunoglobulin G
keywords: Enterotoxigenic Escherichia coli F41; Lactobacillus casei; Mucosal immunization; Passive protection