title: miR-30b regulates migration and invasion of human colorectal cancer via SIX1.
abstract: CRC (colorectal cancer) is one of the most malignant tumours in both developing and developed countries. It is estimated that 60% of CRC patients have liver metastasis. In the present study, we show that miR-30b is an important regulator in human CRC migration and invasion, which are vital steps in CRC liver metastasis. miR-30b was significantly down-regulated in primary CRC specimens compared with normal tissues. Furthermore, miR-30b was much lower in liver metastasis tissues than in CRCs. We validated SIX1 (SIX homeobox 1), a member of the SIX homeodomain family of transcription factors and an EMT (epithelial-mesenchymal transition)-promoting gene, as the direct target of miR-30b. Forced expression of miR-30b inhibited CRC cell migration and invasion in vitro via its target gene SIX1. Furthermore, an inverse correlation between expression of SIX1 and miR-30b has been observed both in primary CRC specimens and liver metastasis. Taken together, miR-30b plays an important role in mediating metastatic related behaviour in CRC. miR-30b may serve as a potential diagnostic marker and therapeutic target for patients with CRC in the future.
authors.affiliation: *Department of Surgery and Department of Oncology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.affiliation: †Department of Anesthesiology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200080, China.
authors.forename: Zifeng
authors.initials: Z
authors.lastname: Xu
authors.affiliation: ‡Department of Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China.
authors.forename: Huanlong
authors.initials: H
authors.lastname: Qin
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Gao
authors.forename: Lu
authors.initials: L
authors.lastname: Gao
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D057809:HEK293 Cells; D018398:Homeodomain Proteins / Q000096:biosynthesis*; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D009361:Neoplasm Invasiveness / Q000473:pathology
chemical_list: D018398:Homeodomain Proteins; C546086:MIRN30b microRNA, human; D035683:MicroRNAs; C099236:SIX1 protein, human