title: Microfluidic-based manufacture of siRNA-lipid nanoparticles for therapeutic applications.
abstract: A simple, efficient, and scalable manufacturing technique is required for developing siRNA-lipid nanoparticles (siRNA-LNP) for therapeutic applications. In this chapter we describe a novel microfluidic-based manufacturing process for the rapid manufacture of siRNA-LNP, together with protocols for characterizing the size, polydispersity, RNA encapsulation efficiency, RNA concentration, and total lipid concentration of the resultant nanoparticles.
authors.affiliation: University of British Columbia, Vancouver, BC, Canada.
authors.forename: Colin
authors.initials: C
authors.lastname: Walsh
authors.forename: Kevin
authors.initials: K
authors.lastname: Ou
authors.forename: Nathan M
authors.initials: NM
authors.lastname: Belliveau
authors.forename: Tim J
authors.initials: TJ
authors.lastname: Leaver
authors.forename: Andre W
authors.initials: AW
authors.lastname: Wild
authors.forename: Jens
authors.initials: J
authors.lastname: Huft
authors.forename: Paulo J
authors.initials: PJ
authors.lastname: Lin
authors.forename: Sam
authors.initials: S
authors.lastname: Chen
authors.forename: Alex K
authors.initials: AK
authors.lastname: Leung
authors.forename: Justin B
authors.initials: JB
authors.lastname: Lee
authors.forename: Carl L
authors.initials: CL
authors.lastname: Hansen
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Taylor
authors.forename: Euan C
authors.initials: EC
authors.lastname: Ramsay
authors.forename: Pieter R
authors.initials: PR
authors.lastname: Cullis
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000737:chemistry*; D004339:Drug Compounding / Q000295:instrumentation / Q000379:methods; D016503:Drug Delivery Systems / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D044085:Microfluidics / Q000295:instrumentation*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010713:Phosphatidylcholines / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000737:chemistry*
chemical_list: D010713:Phosphatidylcholines; D034741:RNA, Small Interfering; D002784:Cholesterol; C010942:1,2-distearoyllecithin