title: Antibody humanization methods - a review and update.
abstract: This article reviews recent advances achieved during recent years on various aspects of antibody humanization theories and techniques. Common methods for producing humanized antibodies including framework-homology-based humanization, germline humanization, complementary determining regions (CDR)-homology-based humanization and specificity determining residues (SDR) grafting, as well as advantages and disadvantages of each of these methods and their applications are discussed.
authors.affiliation: a Biotechnology Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Yaghoub
authors.initials: Y
authors.lastname: Safdari
authors.forename: Safar
authors.initials: S
authors.lastname: Farajnia
authors.forename: Mohammad
authors.initials: M
authors.lastname: Asgharzadeh
authors.forename: Masoumeh
authors.initials: M
authors.lastname: Khalili
mesh_terms: D000595:Amino Acid Sequence; D061067:Antibodies, Monoclonal, Humanized / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000276:immunology* / Q000627:therapeutic use; D022801:Complementarity Determining Regions / Q000276:immunology*; D005854:Germ Cells / Q000276:immunology; D006801:Humans; D015202:Protein Engineering / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D022801:Complementarity Determining Regions