title: Poly(ADP-ribose)polymerase activity controls plant growth by promoting leaf cell number.
abstract: A changing global environment, rising population and increasing demand for biofuels are challenging agriculture and creating a need for technologies to increase biomass production. Here we demonstrate that the inhibition of poly (ADP-ribose) polymerase activity is a promising technology to achieve this under non-stress conditions. Furthermore, we investigate the basis of this growth enhancement via leaf series and kinematic cell analysis as well as single leaf transcriptomics and plant metabolomics under non-stress conditions. These data indicate a regulatory function of PARP within cell growth and potentially development. PARP inhibition enhances growth of Arabidopsis thaliana by enhancing the cell number. Time course single leaf transcriptomics shows that PARP inhibition regulates a small subset of genes which are related to growth promotion, cell cycle and the control of metabolism. This is supported by metabolite analysis showing overall changes in primary and particularly secondary metabolism. Taken together the results indicate a versatile function of PARP beyond its previously reported roles in controlling plant stress tolerance and thus can be a useful target for enhancing biomass production.
authors.affiliation: Bayer CropScience NV, Innovation Center, Zwijnaarde, Belgium ; Department of Molecular Systems Biology (MOSYS), University of Vienna, Vienna, Austria.
authors.forename: Philipp
authors.initials: P
authors.lastname: Schulz
authors.affiliation: Bayer CropScience NV, Innovation Center, Zwijnaarde, Belgium.
authors.forename: Karel
authors.initials: K
authors.lastname: Jansseune
authors.affiliation: Max-Planck-Institute of Molecular Plant Physiology, Potsdam-Golm, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Degenkolbe
authors.affiliation: Max-Planck-Institute of Molecular Plant Physiology, Potsdam-Golm, Germany.
authors.forename: Michaël
authors.initials: M
authors.lastname: Méret
authors.affiliation: Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium ; Department of Plant Systems Biology, VIB, Ghent, Belgium.
authors.forename: Hannes
authors.initials: H
authors.lastname: Claeys
authors.affiliation: Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium ; Department of Plant Systems Biology, VIB, Ghent, Belgium.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Skirycz
authors.affiliation: Department of Molecular Systems Biology (MOSYS), University of Vienna, Vienna, Austria.
authors.forename: Markus
authors.initials: M
authors.lastname: Teige
authors.affiliation: Max-Planck-Institute of Molecular Plant Physiology, Potsdam-Golm, Germany.
authors.forename: Lothar
authors.initials: L
authors.lastname: Willmitzer
authors.affiliation: Bayer CropScience NV, Innovation Center, Zwijnaarde, Belgium.
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Hannah
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D001549:Benzamides / Q000494:pharmacology; D056804:Biofuels; D018533:Biomass; D002453:Cell Cycle / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D010940:Plant Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D059467:Transcriptome
chemical_list: D001549:Benzamides; D056804:Biofuels; D004791:Enzyme Inhibitors; D010940:Plant Proteins; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases; C025159:3-methoxybenzamide