title: Dai Huang Fu Zi Tang could ameliorate intestinal injury in a rat model of hemorrhagic shock by regulating intestinal blood flow and intestinal expression of p-VASP and ZO-1.
abstract: BACKGROUND
  Dai Huang Fu Zi Tang (DHFZT), an oriental herbal formula, has long been used clinically in treatment of intestinal obstruction, acute pancreatitis, cholecystalgia and chronic diarrhea for thousands of years. Recent studies have demonstrated that DHFZT can reduce intestinal pathological injury and the concentration of enterogenous endotoxin in an animal model. But the underlying mechanism has not been fully elucidated.
  
  
  METHODS
  SD male rats in adult were used to model HS and treated with DHFZT. The serum concentration of endotoxin were analyzed by dynamic turbidimetric method. In addition, the blood flow of small intestine were measured using laser speckle technique. Phosphorylated vasodilator-stimulated phosphoprotein (p-VASP) and zonula occludens (ZO)-1 protein, intestinal fatty acid binding protein (IFABP) were measured by Western Blotting, RT-PCR, ELISA respectively.
  
  
  RESULTS
  Present study showed that DHFZT markedly elevated the blood flow of small intestine, protected the intestinal barrier function by up-regulating the expression of ZO-1 protein and down-regulating expression of p-VASP, and notely decreased serum concentration of IFABP and endotoxin in HS.
  
  
  CONCLUSIONS
  These results reveal that DHFZT improves intestinal blood flow, protects the intestinal barrier function, and ameliorates intestinal endotoxaemia in rats with HS.
authors.affiliation: Department of Emergency Medicine, Zhongshan Hospital, Dalian University, Dalian 116001, China. dllxg@126.com.
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Lu
authors.forename: Xin
authors.initials: X
authors.lastname: Kang
authors.forename: Libin
authors.initials: L
authors.lastname: Zhan
authors.forename: Chunyu
authors.initials: C
authors.lastname: Lv
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Fan
authors.forename: Yingli
authors.initials: Y
authors.lastname: Wang
authors.forename: Robbie
authors.initials: R
authors.lastname: Ali
authors.forename: Chang
authors.initials: C
authors.lastname: Lv
authors.forename: Siyao
authors.initials: S
authors.lastname: Li
authors.forename: Jinhai
authors.initials: J
authors.lastname: Mu
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000098:blood supply* / Q000187:drug effects*; D008297:Male; D008840:Microfilament Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow / Q000187:drug effects*; D012771:Shock, Hemorrhagic / Q000188:drug therapy* / Q000378:metabolism*; D062826:Zonula Occludens-1 Protein / Q000378:metabolism*; D000098662:Vasodilator-Stimulated Phosphoprotein
chemical_list: D015815:Cell Adhesion Molecules; D004365:Drugs, Chinese Herbal; D008840:Microfilament Proteins; D010750:Phosphoproteins; D062826:Zonula Occludens-1 Protein; D000098662:Vasodilator-Stimulated Phosphoprotein