title: [The mechanism of inhibition effect of adenovirus-mediated ING4 on human lung adenocarcinoma xenografts in nude mice].
abstract: OBJECTIVE
  The inhibitor of growth 4 (ING4) is an important tumor suppressive gene.It has been proven that ING4 could inhibite the proliferation of many tumors. e aim of this study is to investigate the inhibitory effect and anti-cancer mechanism of adenovirus-mediated ING4 gene on SPC-A1 human lung adenocarcinoma in nude mice.
  
  
  METHODS
  A human lung adenocarcinoma xenograft model was established with SPC-A1 cells in nude mice. A total of 15 tumor-bearing nude mice were randomly divided into three groups, namely, PBS, Ad-GFP, and Ad-ING4. e mice in the three groups were intratumorally injected every other day. Their tumor volumes were continually recorded. The treatment tumors were then removed from the mice and weighed. Tumor inhibition rates were calculated. Cell apoptosis was examined by TUNEL method. Caspase-3, COX-2, Fas, and FasL expressions were investigated by immunohistochemistry SP assay.
  
  
  RESULTS
  Both tumor weight and volume in the Ad-ING4 group were significantly decreased. The tumor inhibition rate of the mice in the Ad-ING4 group (33.17% ± 5.24%) was statistically different from that of the mice in the Ad-GFP group (1.31% ± 0.31%; P<0.05). The apoptotic index of the mice in the Ad-ING4 group (69.23% ± 6.53%) was also significantly different from those in PBS (17.04% ± 1.10%) and Ad-GFP groups (18.81% ± 1.93%; P<0.05). Based on immunohistochemistry SP assay, the results showed that Ad-ING4 may not only upregulate the expressions of caspase-3, Fas, and FasL but also downregulate the expression of COX-2.
  
  
  CONCLUSIONS
  ING4 gene elicited a remarkable growth inhibitory e-ect on human lung adenocarcinoma xenografts in nude mice. e mechanism is possibly related to an increase in tumor cell apoptosis.
authors.affiliation: Department of Respiratory, Second People's Hospital of Changshu, Changshu 215500, China.
authors.forename: Jinhong
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Cell and Molecular Biology, College of Medicine, Soochow University, Suzhou 215123, China.
authors.forename: Jicheng
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Respiratory Medicine, First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Ling
authors.affiliation: Department of Respiratory Medicine, First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Daguo
authors.initials: D
authors.lastname: Zhao
authors.affiliation: Department of Cell and Molecular Biology, College of Medicine, Soochow University, Suzhou 215123, China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Department of Respiratory, Second People's Hospital of Changshu, Changshu 215500, China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: You
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D000077192:Adenocarcinoma of Lung; D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism; D000818:Animals; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D015316:Genetic Therapy / Q000295:instrumentation; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D047368:Tumor Burden; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays
chemical_list: D018797:Cell Cycle Proteins; D018398:Homeodomain Proteins; C483230:ING4 protein, human; D025521:Tumor Suppressor Proteins; D053148:Caspase 3