title: Phylogenetic analysis of the Black Stork Ciconia nigra (Ciconiiformes: Ciconiidae) based on complete mitochondrial genome.
abstract: The Black Stork, Ciconia nigra belongs to family Ciconiidae, which is evaluated as Least Concern by IUCN. In this study, the complete mitochondrial genome of C. nigra was first sequenced and characterized, which was 17,795 bp in length. The mt-genome has tandem repeats of 80 bp and 78 bp repeat units, and AAACAAC and AAACAAACAAC tandem repeats in D-loop region. It is notable that a single extra base "C" at position 174 was inserted in gene ND3. Bayesian inference, maximum likelihood methods were used to construct phylogenetic trees based on 12 heavy-strand protein-coding genes. Phylogenetic analyses showed that Ardeidae diverged earlier than Ciconiidae, Cathartida and Threskiornithidae, and Ciconiidae had closest relationship to Cathartida. C. nigra diverged first among three Ciconia birds.
authors.affiliation: a Key Laboratory of Bio-resources and Eco-environment (Ministry of Education) and.
authors.forename: Mengyao
authors.initials: M
authors.lastname: Liu
authors.affiliation: a Key Laboratory of Bio-resources and Eco-environment (Ministry of Education) and.
authors.forename: Chunlan
authors.initials: C
authors.lastname: Kang
authors.affiliation: a Key Laboratory of Bio-resources and Eco-environment (Ministry of Education) and.
authors.forename: Chaochao
authors.initials: C
authors.lastname: Yan
authors.affiliation: a Key Laboratory of Bio-resources and Eco-environment (Ministry of Education) and.
authors.forename: Ting
authors.initials: T
authors.lastname: Huang
authors.affiliation: a Key Laboratory of Bio-resources and Eco-environment (Ministry of Education) and.
authors.forename: Xuhao
authors.initials: X
authors.lastname: Song
authors.affiliation: b Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife , College of Life Sciences, Sichuan University , Chengdu , P. R. China.
authors.forename: Xiuyue
authors.initials: X
authors.lastname: Zhang
authors.affiliation: b Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife , College of Life Sciences, Sichuan University , Chengdu , P. R. China.
authors.forename: Bisong
authors.initials: B
authors.lastname: Yue
authors.affiliation: b Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife , College of Life Sciences, Sichuan University , Chengdu , P. R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zeng
mesh_terms: D000818:Animals; D001717:Birds / Q000235:genetics*; D054629:Genome, Mitochondrial / Q000235:genetics*; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D017422:Sequence Analysis, DNA
keywords: Ciconia nigra; complete mitochondrial genome; phylogeny