title: Spatiotemporal pattern of RNA-binding motif protein 3 expression after spinal cord injury in rats.
abstract: RNA-binding motif protein 3 (RBM3) belongs to a very small group of cold inducible proteins with anti-apoptotic and proliferative functions. To elucidate the expression and possible function of RBM3 in central nervous system (CNS) lesion and repair, we performed a spinal cord injury (SCI) model in adult rats. Western blot analysis revealed that RBM3 level significantly increased at 1 day after damage, and then declined during the following days. Immunohistochemistry further confirmed that RBM3 immunoactivity was expressed at low levels in gray and white matters in normal condition and increased at 1 day after SCI. Besides, double immunofluorescence staining showed RBM3 was primarily expressed in the neurons and a few of astrocytes in the normal group. While after injury, the expression of RBM3 increased both in neurons and astrocytes at 1 day. We also examined the expression profiles of proliferating cell nuclear antigen (PCNA) and active caspase-3 in injured spinal cords by western blot. Importantly, double immunofluorescence staining revealed that cell proliferation evaluated by PCNA appeared in many RBM3-expressing cells and rare caspase-3 was observed in RBM3-expressing cells at 1 day after injury. Our data suggested that RBM3 might play important roles in CNS pathophysiology after SCI.
authors.affiliation: Department of Orthopaedics, The Second Affiliated Hospital of Nantong University, Nantong, 226001, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.forename: Dawei
authors.initials: D
authors.lastname: Xu
authors.forename: Gang
authors.initials: G
authors.lastname: Cai
authors.forename: Xinhui
authors.initials: X
authors.lastname: Zhu
authors.forename: Ming
authors.initials: M
authors.lastname: Qian
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Cui
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001253:Astrocytes / Q000378:metabolism*; D004195:Disease Models, Animal; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D009474:Neurons / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000473:pathology
chemical_list: D018809:Proliferating Cell Nuclear Antigen; C533319:RBM3 protein, rat; D016601:RNA-Binding Proteins