title: High-throughput cloning for biophysical applications.
abstract: Efficient high-throughput gene cloning represents a critical first step for conducting functional and structural proteomics in the post-genomic era. The ligation-independent cloning (LIC) method has been almost universally adopted by large structural biology centers as a component of high-throughput structure determination pipelines. The LIC platform is easy to use, of low cost, and rapid, and importantly, it is easily adapted to 96- or 384-well format, thereby facilitating automation. Procedures are described for 96-well format cloning using the LIC technology.
authors.affiliation: J. Craig Venter Institute, 9704 Medical Center Dr., Rockville, MD, 20850, USA, kkwon@jcvi.org.
authors.forename: Keehwan
authors.initials: K
authors.lastname: Kwon
authors.forename: Scott N
authors.initials: SN
authors.lastname: Peterson
mesh_terms: D055592:Biophysical Phenomena; D003001:Cloning, Molecular / Q000379:methods*; D057166:High-Throughput Screening Assays; D008967:Molecular Biology / Q000379:methods*; D040681:Structural Homology, Protein