title: Rapid detection of β-lactamase-hydrolyzing extended-spectrum cephalosporins in Enterobacteriaceae by use of the new chromogenic βLacta test.
abstract: The chromogenic βLacta test developed for the rapid detection of β-lactamase-hydrolyzing extended-spectrum cephalosporins in Enterobacteriaceae revealed good performance with extended-spectrum β-lactamase (ESBL) producers (97.5% true-positive results). However, false-negative results occurred with chromosomal AmpC hyperproducers and plasmid AmpC producers, whereas uninterpretable results were mostly due to VIM-1 carbapenemase producers and possibly low levels of expressed ESBLs.
authors.affiliation: Servicio de Microbiología, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigaciones Sanitarias, Madrid, Spain.
authors.forename: María Isabel
authors.initials: MI
authors.lastname: Morosini
authors.forename: María
authors.initials: M
authors.lastname: García-Castillo
authors.forename: Marta
authors.initials: M
authors.lastname: Tato
authors.forename: Desirèe
authors.initials: D
authors.lastname: Gijón
authors.forename: Aránzazu
authors.initials: A
authors.lastname: Valverde
authors.forename: Patricia
authors.initials: P
authors.lastname: Ruiz-Garbajosa
authors.forename: Rafael
authors.initials: R
authors.lastname: Cantón
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D002511:Cephalosporins / Q000494:pharmacology*; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000235:genetics*; D004756:Enterobacteriaceae Infections / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology*; D006868:Hydrolysis; D008826:Microbial Sensitivity Tests / Q000379:methods; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D002511:Cephalosporins; D001618:beta-Lactamases; C063912:carbapenemase