title: Series of new β-cyclodextrin-cored starlike carriers for gene delivery.
abstract: The development of safe and effective β-cyclodextrin (β-CD)-cored cationic star gene carriers has attracted considerable attention. In this work, a series of star-shaped hemocompatible CD-PGPP, CD-PGAEPP, and CD-PGAPP vectors composed of β-CD cores and piperazine (PP)-, N-(aminoethyl)piperazine (AEPP)-, or N-(3-aminopropyl)-2-pyrrolidinone (APP)-functionalized poly(glycidyl methacrylate) arms were successfully proposed and compared for highly efficient gene delivery. Such star carriers possess plentiful secondary amine, tertiary amine, and nonionic hydroxyl groups. CD-PGPP, CD-PGAEPP, and CD-PGAPP were effective in condensing plasmid DNA into nanoparticles, whose sizes were 100-200 nm and positive ζ potentials were 25-40 mV at nitrogen/phosphate (N/P) ratios of 10 and above. CD-PGPP, CD-PGAEPP, and CD-PGAPP showed significantly lower cytotoxicity than control poly(ethylenimine) (PEI; ∼25 kDa). At most N/P ratios, CD-PGAPP exhibited better gene transfection performance than CD-PGPP and CD-PGAEPP particularly in HepG2 cells. More importantly, in comparison with PEI, all of the CD-PGPP, CD-PGAEPP, and CD-PGAPP vectors did not cause undesirable hemolysis.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Key Laboratory of Carbon Fiber and Functional Polymers (Beijing University of Chemical Technology), Ministry of Education, Beijing Laboratory of Biomedical Materials, College of Materials Science & Engineering, Beijing University of Chemical Technology , Beijing 100029 China.
authors.forename: R Q
authors.initials: RQ
authors.lastname: Li
authors.forename: Y L
authors.initials: YL
authors.lastname: Niu
authors.forename: N N
authors.initials: NN
authors.lastname: Zhao
authors.forename: B R
authors.initials: BR
authors.lastname: Yu
authors.forename: C
authors.initials: C
authors.lastname: Mao
authors.forename: F J
authors.initials: FJ
authors.lastname: Xu
mesh_terms: D002460:Cell Line; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry; D010957:Plasmids / Q000737:chemistry / Q000235:genetics*; D014162:Transfection / Q000295:instrumentation*; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D047392:beta-Cyclodextrins; C031215:betadex