title: Assessment of the BD MGIT TBc identification test for the detection of Mycobacterium tuberculosis complex in a network of mycobacteriology laboratories.
abstract: We evaluate the performance of the TBcID assay in a panel of 100 acid-fast bacilli cultures. Sixty-four isolates were TBcID positive for Mycobacterium tuberculosis complex (MTBC), whereas 36 gave negative results. These included 28 nontuberculous mycobacteria, one nonmycobacterial isolate, one M. tuberculosis, and six M. bovis BCG strains. This corresponds to a sensitivity of 90.14%, specificity of 100%, and positive and negative predictive values of 100% and 80.55%, respectively. The test is rapid, easy to perform and interpret, and does not require sample preparation or instrumentation. However, a negative result does not exclude the presence of a strain belonging to MTBC, especially when mutations in mpb64 gene are present or some M. bovis BCG strains are isolated. The TBcID showed potential to assist in the identification of MTBC when the implementation and usage of molecular methods are often not possible, principally in resource-limited countries.
authors.affiliation: Grupo de Micobactérias, Unidade de Microbiologia Médica, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (IHMT/UNL), Rua da Junqueira 100, 1349-008 Lisboa, Portugal.
authors.forename: Diana
authors.identifier: 0000-0003-2375-2726
authors.initials: D
authors.lastname: Machado
authors.affiliation: Grupo de Micobactérias, Unidade de Microbiologia Médica, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (IHMT/UNL), Rua da Junqueira 100, 1349-008 Lisboa, Portugal.
authors.forename: Jorge
authors.initials: J
authors.lastname: Ramos
authors.affiliation: Grupo de Micobactérias, Unidade de Microbiologia Médica, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (IHMT/UNL), Rua da Junqueira 100, 1349-008 Lisboa, Portugal ; Centro de Recursos Microbiológicos (CREM), UNL, 2829-516 Caparica, Portugal.
authors.forename: Isabel
authors.initials: I
authors.lastname: Couto
authors.affiliation: Instituto Nacional de Saúde, Ministério da Saúde de Moçambique, 264 Cidade de Maputo, Mozambique.
authors.forename: Nureisha
authors.identifier: 0000-0002-2563-1605
authors.initials: N
authors.lastname: Cadir
authors.affiliation: Instituto Nacional de Saúde, Ministério da Saúde de Moçambique, 264 Cidade de Maputo, Mozambique.
authors.forename: Inácio
authors.initials: I
authors.lastname: Narciso
authors.affiliation: Programa Nacional de Controlo da Tuberculose, Ministério da Saúde de Moçambique, 264 Cidade de Maputo, Mozambique.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Coelho
authors.affiliation: Instituto Nacional de Saúde, Ministério da Saúde de Moçambique, 264 Cidade de Maputo, Mozambique.
authors.forename: Sofia
authors.initials: S
authors.lastname: Viegas
authors.affiliation: Grupo de Micobactérias, Unidade de Microbiologia Médica, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (IHMT/UNL), Rua da Junqueira 100, 1349-008 Lisboa, Portugal ; Centro de Malária e Outras Doenças Tropicais/LA, IHMT/UNL, Rua da Junqueira 100, 1349-008 Lisboa, Portugal.
authors.forename: Miguel
authors.identifier: 0000-0001-9676-6251
authors.initials: M
authors.lastname: Viveiros
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D003470:Culture Media; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic
chemical_list: D003470:Culture Media; D011933:Reagent Kits, Diagnostic