title: Development of an indirect competitive assay-based aptasensor for highly sensitive detection of tetracycline residue in honey.
abstract: Tetracycline (TC) is widely used for prevention and control of animal diseases for its broad spectrum antimicrobial activity and low cost, but its abuse can seriously affect human health and may result in trade loss. Thus there is an imperative need to develop high-performing analytical technique for TC detection. In this study, we developed a biosensor based on an indirect competitive enzyme-linked aptamer assay (ic-ELAA). A 76mer single-stranded DNA (ssDNA) aptamer, selected by Systematic Evolution of Ligands by Exponential Enrichment (SELEX), was applied for the recognition and detection of TC in honey. The limit of detection was 9.6×10(-3) ng/mL with a linear working range from 0.01 to 100 ng/mL toward TC in honey, and a mean recovery rate of 93.23% in TC-spiked honey was obtained. This aptasensor can be applied to detect TC residue in food with high sensitivity and simplicity, and it is prospective to develop useful ELAA Kits for TC determination in food.
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Sai
authors.initials: S
authors.lastname: Wang
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yong
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Liu
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Liya
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Qilong
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China. Electronic address: yydong@mail.buct.edu.cn.
authors.forename: Yiyang
authors.initials: Y
authors.lastname: Dong
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods; D004277:DNA, Single-Stranded / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis*; D006722:Honey / Q000032:analysis*; D057230:Limit of Detection; D052156:SELEX Aptamer Technique; D013752:Tetracycline / Q000032:analysis*
chemical_list: D000900:Anti-Bacterial Agents; D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D013752:Tetracycline
keywords: Aptamer; Aptasensor; Enzyme-linked aptamer assay; Honey analysis; Tetracycline