title: A new murine model to study musculoskeletal tuberculosis (short communication).
abstract: Musculoskeletal tuberculosis (TB) is a severe extrapulmonary manifestation of chronic Mycobacterium (M.) tuberculosis infection. Considering increasing incidence, multi-drug resistance and associated treatment difficulties, more preclinical research is needed. In this study we developed a murine model for musculoskeletal TB. Mice, intranasally infected with M. tuberculosis, were sacrificed after ten months. Mycobacterial growth was detected in lung and femur homogenates. Ziehl-Neelsen staining of paraffin-embedded femurs showed acid-fast rods in the myelum and Magnetic Resonance Imaging demonstrated osteomyelitis and macronodular tuberculomas. This new murine model of musculoskeletal TB might be of value to further investigate immunologic and radiologic responses.
authors.affiliation: Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center/University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands; Center for Experimental and Molecular Medicine (CEMM), Academic Medical Center/University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands. Electronic address: l.m.kager@amc.uva.nl.
authors.forename: Liesbeth M
authors.initials: LM
authors.lastname: Kager
authors.affiliation: Department of Radiology, Academic Medical Center/University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands. Electronic address: j.h.runge@amc.uva.nl.
authors.forename: Jurgen H
authors.initials: JH
authors.lastname: Runge
authors.affiliation: Department of Radiology, Academic Medical Center/University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands. Electronic address: a.j.nederveen@amc.uva.nl.
authors.forename: Aart J
authors.initials: AJ
authors.lastname: Nederveen
authors.affiliation: Department of Pathology, Academic Medical Center/University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands. Electronic address: j.j.roelofs@amc.uva.nl.
authors.forename: Joris J T H
authors.initials: JJ
authors.lastname: Roelofs
authors.affiliation: Department of Radiology, Academic Medical Center/University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands. Electronic address: j.stoker@amc.uva.nl.
authors.forename: Jaap
authors.initials: J
authors.lastname: Stoker
authors.affiliation: Department of Radiology, Academic Medical Center/University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands. Electronic address: m.maas@amc.uva.nl.
authors.forename: Mario
authors.initials: M
authors.lastname: Maas
authors.affiliation: Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center/University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands; Center for Experimental and Molecular Medicine (CEMM), Academic Medical Center/University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands; Division of Infectious Diseases, Academic Medical Center/University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands. Electronic address: t.vanderpoll@amc.uva.nl.
authors.forename: Tom
authors.initials: T
authors.lastname: van der Poll
mesh_terms: D000818:Animals; D050281:Bones of Lower Extremity / Q000382:microbiology; D004195:Disease Models, Animal; D005260:Female; D008168:Lung / Q000382:microbiology; D008279:Magnetic Resonance Imaging; D008810:Mice, Inbred C57BL; D009140:Musculoskeletal Diseases / Q000382:microbiology*; D009169:Mycobacterium tuberculosis; D010019:Osteomyelitis / Q000382:microbiology; D014376:Tuberculosis / Q000382:microbiology*; D014394:Tuberculosis, Osteoarticular / Q000382:microbiology; D014399:Tuberculosis, Spinal / Q000382:microbiology
keywords: Bone and joint tuberculosis; Magnetic resonance imaging; Mice; Musculoskeletal imaging; Pott's disease; Spinal tuberculosis