title: C/EBP-α, involvement of a novel transcription factor in leptin-induced VCAM-1 production in mouse chondrocytes.
abstract: Leptin and vascular cell adhesion molecules-1 (VCAM-1) are two important mediators in obesity-related osteoarthritis, while the molecular mechanism linking leptin to VCAM-1 production is still obscure. Here we show that leptin upregulates VCAM-1 mRNA and protein levels in a time- and dose-dependent manner. Mechanistically, leptin induces VCAM-1 promoter activity by increasing the expression of C/EBP-α and facilitating its binding to a newly identified element in the VCAM-1 gene. Gain or loss of function studies reveal a regulatory role of C/EBP-α on VCAM-1 expression. Finally, elevated plasma leptin level correlates to increased C/EBP-α and VCAM-1 production in chondrocytes from obese mice.
authors.affiliation: Department of Orthopaedics, Chengdu Military General Hospital, No.270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan, 610083, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Kang
authors.affiliation: Department of Orthopaedics, Chengdu Military General Hospital, No.270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan, 610083, China.
authors.forename: Qing-Yun
authors.initials: QY
authors.lastname: Xie
authors.affiliation: Department of Orthopaedics, Chengdu Military General Hospital, No.270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan, 610083, China.
authors.forename: Jing-Song
authors.initials: JS
authors.lastname: Zhou
authors.affiliation: Department of Orthopaedics, Chengdu Military General Hospital, No.270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan, 610083, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedics, Chengdu Military General Hospital, No.270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan, 610083, China.
authors.forename: Dong-Fa
authors.initials: DF
authors.lastname: Liao
authors.affiliation: Department of Orthopaedics, Chengdu Military General Hospital, No.270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan, 610083, China.
authors.forename: Hong-Hua
authors.initials: HH
authors.lastname: Wu
authors.affiliation: Department of Orthopaedics, Chengdu Military General Hospital, No.270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan, 610083, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Department of Orthopaedics, Chengdu Military General Hospital, No.270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan, 610083, China. Electronic address: panxmpaper@gmail.com.
authors.forename: Xian-Ming
authors.initials: XM
authors.lastname: Pan
authors.affiliation: Department of Orthopaedics, Chengdu Military General Hospital, No.270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan, 610083, China. Electronic address: quanyi56paper@gmail.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Quan
mesh_terms: D000818:Animals; D001665:Binding Sites; D022762:CCAAT-Enhancer-Binding Proteins / Q000502:physiology*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism*; D006801:Humans; D020738:Leptin / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000097:blood; D061251:Primary Cell Culture; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics* / Q000378:metabolism
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; C530718:CEBPA protein, mouse; D020738:Leptin; D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1
keywords: C/EBP-α; Leptin; Osteoarthritis; VCAM-1