title: Maternal plasma prolidase, matrix metalloproteinases 1 and 13, and oxidative stress levels in pregnancies complicated by preterm premature rupture of the membranes and chorioamnionitis.
abstract: OBJECTIVE
  This study aimed to investigate the role of various biochemical markers in preterm premature rupture of membranes (PPROM) and in prediction of chorioamnionitis in patients with PPROM.
  
  
  METHODS
  This case-control study included a total of 100 pregnant women at 26-34 gestational weeks. Of these women, 50 were healthy and 50 had PPROM. The biochemical markers in the maternal plasma including prolidase, matrix metalloproteinase (MMP) 1 and 13, total oxidative status (TOS), total antioxidant capacity (TAC), glutathione peroxidase (GPx), catalase (CAT), paraoxonase-1 (PON-1), tumor necrosis factor alpha (TNF-α), and high sensitive C-reactive protein (hs-CRP) were assayed. These levels were compared between the PPROM and control groups and between women with or without chorioamnionitis in the PPROM group.
  
  
  RESULTS
  Compared to the control group, the levels of prolidase, MMP-13, and TOS were significantly higher (p values <0.001, 0.020, and 0.035, respectively) and those of TAC and PON-1 were significantly lower in the maternal plasma of the PPROM group (p values=0.012 and <0.001, respectively). The plasma prolidase and TOS levels were significantly higher (p values=0.033 and 0.005, respectively) and the plasma TAC and PON-1 levels were significantly lower in women with chorioamnionitis as compared with the corresponding values in women without chorioamnionitis in the PPROM group (p values =0.041 and 0.048, respectively). The multivariate logistic regression analysis observed that prolidase, TAC, and PON-1 were important markers for the presence of PPROM and prolidase and TOS were important markers for predicting chorioamnionitis.
  
  
  CONCLUSIONS
  This study suggested that maternal plasma prolidase, TAC, and PON-1 may be useful for the diagnosis of PPROM, and prolidase and TOS may be used to predict chorioamnionitis in patients with PPROM.
authors.affiliation: Department of Gynecology and Obstetrics, Faculty of Medicine, Dicle University, Diyarbakır, Turkey.
authors.forename: Hatice Ender
authors.initials: HE
authors.lastname: Soydinç
authors.affiliation: Department of Gynecology and Obstetrics, Faculty of Medicine, Dicle University, Diyarbakır, Turkey.
authors.forename: Muhammet Erdal
authors.initials: ME
authors.lastname: Sak
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Dicle University, Diyarbakır, Turkey.
authors.forename: Osman
authors.initials: O
authors.lastname: Evliyaoğlu
authors.affiliation: Department of Gynecology and Obstetrics, Faculty of Medicine, Dicle University, Diyarbakır, Turkey.
authors.forename: Mehmet Sıddık
authors.initials: MS
authors.lastname: Evsen
authors.affiliation: Department of Gynecology and Obstetrics, Faculty of Medicine, Dicle University, Diyarbakır, Turkey.
authors.forename: Abdulkadir
authors.initials: A
authors.lastname: Turgut
authors.affiliation: Department of Gynecology and Obstetrics, Faculty of Medicine, Dicle University, Diyarbakır, Turkey.
authors.forename: Ali
authors.initials: A
authors.lastname: Ozler
authors.affiliation: Department of Gynecology and Obstetrics, Faculty of Medicine, Dicle University, Diyarbakır, Turkey.
authors.forename: Hayrettin
authors.initials: H
authors.lastname: Tay
authors.affiliation: Department of Gynecology and Obstetrics, Faculty of Medicine, Dicle University, Diyarbakır, Turkey.
authors.forename: Talip
authors.initials: T
authors.lastname: Gül
keywords: Preterm premature rupture of membranes; maternal plasma; matrix metalloproteinase; oxidative stress; prolidase