title: Efficient delivery of lentiviral vectors into resting human CD4 T cells.
abstract: Resting human CD4 T cells are highly resistant to transfection or infection with lentiviral vectors derived from the human immunodeficiency virus. We now describe a flexible and efficient approach involving virus-like particles containing simian immunodeficiency virus lentiviral gene product protein X and pseudotyping with CXCR4-tropic HIV Env. This method permits effective genetic manipulation of these cells while preserving their naturally quiescent state. This technology can also be extended to primary lymphoid cultures where authentic cellular composition and functional relationships are preserved.
authors.affiliation: Virology & Immunology, J. David Gladstone Institutes, San Francisco, CA, USA.
authors.forename: X
authors.initials: X
authors.lastname: Geng
authors.affiliation: Virology & Immunology, J. David Gladstone Institutes, San Francisco, CA, USA.
authors.forename: G
authors.initials: G
authors.lastname: Doitsh
authors.affiliation: Virology & Immunology, J. David Gladstone Institutes, San Francisco, CA, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Virology & Immunology, J. David Gladstone Institutes, San Francisco, CA, USA.
authors.forename: N L K
authors.initials: NL
authors.lastname: Galloway
authors.affiliation: 1] Virology & Immunology, J. David Gladstone Institutes, San Francisco, CA, USA [2] Department of Medicine, University of California, San Francisco, San Francisco, CA, USA [3] Department of Microbiology and Immunology, University of California, San Francisco, CA, USA.
authors.forename: W C
authors.initials: WC
authors.lastname: Greene
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism* / Q000473:pathology; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D015497:HIV-1 / Q000235:genetics; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D019718:Receptors, CXCR4 / Q000235:genetics / Q000627:therapeutic use; D015302:Simian Immunodeficiency Virus; D014764:Viral Proteins / Q000008:administration & dosage / Q000235:genetics* / Q000627:therapeutic use; D054299:env Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000627:therapeutic use
chemical_list: D019718:Receptors, CXCR4; D014764:Viral Proteins; D054299:env Gene Products, Human Immunodeficiency Virus