title: Oxidative stress induced by copper and iron complexes with 8-hydroxyquinoline derivatives causes paraptotic death of HeLa cancer cells.
abstract: Here, we report the antiproliferative/cytotoxic properties of 8-hydroxyquinoline (8-HQ) derivatives on HeLa cells in the presence of transition metal ions (Cu(2+), Fe(3+), Co(2+), Ni(2+)). Two series of ligands were tested, the arylvinylquinolinic L1-L8 and the arylethylenequinolinic L9-L16, which can all interact with metal ions by virtue of the N,O donor set of 8-HQ; however, only L9-L16 are flexible enough to bind the metal in a multidentate fashion, thus exploiting the additional donor functions. L1-L16 were tested for their cytotoxicity on HeLa cancer cells, both in the absence and in the presence of copper. Among them, the symmetric L14 exhibits the highest differential activity between the ligand alone (IC50 = 23.7 μM) and its copper complex (IC50 = 1.8 μM). This latter, besides causing a significant reduction of cell viability, is associated with a considerable accumulation of the metal inside the cells. Metal accumulation is also observed when the cells are incubated with L14 complexed with other late transition metal ions (Fe(3+), Co(2+), Ni(2+)), although the biological response of HeLa cells is different. In fact, while Ni/L14 and Co/L14 exert a cytostatic effect, both Cu/L14 and Fe/L14 trigger a caspase-independent paraptotic process, which results from the induction of a severe oxidative stress and the unfolded protein response.
authors.affiliation: Dipartimento di Chimica, Università degli Studi di Parma , Viale delle Scienze 17/A, 43123 Parma, Italy.
authors.forename: Amelia
authors.initials: A
authors.lastname: Barilli
authors.forename: Corrado
authors.initials: C
authors.lastname: Atzeri
authors.forename: Irene
authors.initials: I
authors.lastname: Bassanetti
authors.forename: Filippo
authors.initials: F
authors.lastname: Ingoglia
authors.forename: Valeria
authors.initials: V
authors.lastname: Dall'Asta
authors.forename: Ovidio
authors.initials: O
authors.lastname: Bussolati
authors.forename: Monica
authors.initials: M
authors.lastname: Maffini
authors.forename: Claudio
authors.initials: C
authors.lastname: Mucchino
authors.forename: Luciano
authors.initials: L
authors.lastname: Marchiò
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D003300:Copper / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D006912:Hydroxyquinolines / Q000138:chemical synthesis / Q000494:pharmacology*; D007501:Iron / Q000494:pharmacology*; D015394:Molecular Structure; D018384:Oxidative Stress / Q000187:drug effects*; D056811:Unfolded Protein Response
chemical_list: D006912:Hydroxyquinolines; D003300:Copper; D007501:Iron; D020169:Caspases