title: Effect of follicular fluid NO, MDA and GSH levels on in vitro fertilization outcomes.
abstract: OBJECTIVE
  To investigate the biochemical markers such as nitric oxide (NO), malondialdehyde (MDA) and reduced glutathione (GSH), indicators of the oxidative status of the follicle, to predict the outcome of in vitro fertilization.
  
  
  METHODS
  Follicular aspirates of dominant follicles were collected during oocyte retrieval. Biochemical analyses of NO, MDA and GSH were performed on all aspirates.
  
  
  RESULTS
  When the successful and unsuccessful pregnancy groups were compared in terms of NO, MDA and GSH, follicular fluid MDA was significantly higher (p=0.001) and follicular fluid NO level was significantly lower (p=0.039) in the pregnant group. Correlation analysis between oxidative stress and IVF parameters showed that MDA had a positive weak correlation with the number of grade 1 embryos (r=0.271, p=0.033) and fertilization rate (r=0.263, p=0.039). ROC curve analysis found that malondialdehyde has an area under the curve of 0.74 and can predict pregnancy with high sensitivity.
  
  
  CONCLUSIONS
  As malondialdehyde was significantly different in pregnant and non-pregnant women and had a good sensitivity profile in predicting pregnancy, it may be considered a marker for predicting IVF success.
authors.affiliation: IVF Center, Kocaeli University Faculty of Medicine, Kocaeli, Turkey.
authors.forename: Ender
authors.initials: E
authors.lastname: Yalçınkaya
authors.affiliation: Department of Obstetrics and Gynecology, Kocaeli University Faculty of Medicine, Kocaeli, Turkey.
authors.forename: Yiğit
authors.initials: Y
authors.lastname: Cakıroğlu
authors.affiliation: Department of Obstetrics and Gynecology, Kocaeli University Faculty of Medicine, Kocaeli, Turkey.
authors.forename: Emek
authors.initials: E
authors.lastname: Doğer
authors.affiliation: IVF Center, Kocaeli University Faculty of Medicine, Kocaeli, Turkey.
authors.forename: Ozcan
authors.initials: O
authors.lastname: Budak
authors.affiliation: Department of Biochemistry, Kocaeli University Faculty of Medicine, Kocaeli, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Cekmen
authors.affiliation: Department of Obstetrics and Gynecology, Kocaeli University Faculty of Medicine, Kocaeli, Turkey.
authors.forename: Eray
authors.initials: E
authors.lastname: Calışkan
keywords: Follicular fluid; glutathione; in vitro fertilization; malondialdehyde; nitric oxide; pregnancy