title: The clinical utility of a near patient care rapid microarray-based diagnostic test for influenza and respiratory syncytial virus infections in the pediatric setting.
abstract: We evaluated the potential clinical utility of an automated near patient molecular assay Verigene Respiratory Virus Plus (RV+) and rapid immunochromatographic antigen tests (RIAT) in the pediatric setting for diagnosis of influenza and respiratory syncytial virus infections when testing was performed by the pediatrician seeing the patient. Overall, with respect to influenza virus, sensitivity and specificity for RIAT were 70.8% and 100%, respectively, compared to 100% and 96.2%, respectively, for RV+. For respiratory syncytial virus, sensitivity and specificity for RIAT were 78.9% and 100%, respectively, compared to 100% and 100%, respectively, for RV+. When RIAT and RV+ sensitivity for influenza virus was compared based on the time the patient presented after onset of fever, the sensitivity of RIAT at 6 hours was 37.5% compared to 100% for RV+. At 12 hours, RIAT improved to 60.9%. This study confirms the clinical utility of RV+ in the pediatric setting.
authors.affiliation: Department of Pediatrics, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Nakao
authors.affiliation: Department of Pediatrics, Juntendo University School of Medicine, Tokyo, Japan. Electronic address: ken-hisa@juntendo.ac.jp.
authors.forename: Ken
authors.initials: K
authors.lastname: Hisata
authors.affiliation: Department of Pediatrics, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Matsunaga
authors.affiliation: Department of Pediatrics, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Fujimori
authors.affiliation: Department of Pediatrics, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: Naomi
authors.initials: N
authors.lastname: Yoshikawa
authors.affiliation: Department of Pediatrics, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: Mitsutaka
authors.initials: M
authors.lastname: Komatsu
authors.affiliation: Department of Infection Control Science, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Kikuchi
authors.affiliation: East-West Diagnostics/Theranostics, San Francisco, CA, USA.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takahashi
authors.affiliation: Department of Pediatrics, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Shimizu
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D002846:Chromatography, Affinity / Q000379:methods*; D003955:Diagnostic Tests, Routine / Q000379:methods*; D005260:Female; D006801:Humans; D007223:Infant; D007251:Influenza, Human / Q000175:diagnosis*; D008297:Male; D046228:Microarray Analysis / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009975:Orthomyxoviridae / Q000302:isolation & purification; D019095:Point-of-Care Systems; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis*; D018113:Respiratory Syncytial Virus, Human / Q000302:isolation & purification; D012680:Sensitivity and Specificity
keywords: Influenza; Microarray: Immunochromatography; Near patient care test; Pediatrics; Rapid antigen test; Respiratory syncytial virus