title: Uptake of different crystal structures of TiO₂ nanoparticles by Caco-2 intestinal cells.
abstract: The gastrointestinal uptake of different crystal structures of TiO2 was investigated using Caco-2 intestinal cells. Caco-2 monolayers exhibited time-dependent, saturable uptake of Ti from TiO2 exposures of 1 mgl(-1) over 24h, which was influenced by crystal type. Initial uptake rates were 5.3, 3.73, 3.58 and 4.48 nmol mg(-1)protein h(-1) for bulk, P25, anatase and rutile forms respectively. All exposures caused elevations of Ti in the cells relative to the control (ANOVA P<0.05). Electron micrographs of the Caco-2 monolayer showed the presence of particles inside the cells, and energy dispersive spectroscopy (EDS) confirmed the composition as TiO2. Incubating the cells with 120 IU nystatin (putative endocytosis inhibitor) or 100 μmol l(-1) vanadate (ATPase inhibitor) caused large increases in Ti accumulation for all crystal types relative to controls (ANOVA P<0.05), except for the rutile form with vanadate. Incubating the cells with 90 μmol l(-1) genistein (tyrosine kinase inhibitor) or 27 μmol l(-1) chloropromazine (clathrin-mediated endocytosis inhibitor) caused a large decrease in Ti accumulation relative to the controls (ANOVA P<0.05). Cell viability measures were generally good (low LDH leak, normal cell morphology), but there were some changes in the electrolyte composition (K(+), Na(+), Ca(2+), Mg(2+)) of exposed cells relative to controls. A rise in total Ca(2+) concentration in the cells was observed for all TiO2 crystal type exposures. Overall, the data shows that Ti accumulation for TiO2 NP exposure in Caco-2 cells is crystal structure-dependent, and that the mechanism(s) involves endocytosis of intact particles.
authors.affiliation: School of Biomedical & Biological Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA, UK. Electronic address: constantinos.gitrowski@plymouth.ac.uk.
authors.forename: Constantinos
authors.initials: C
authors.lastname: Gitrowski
authors.affiliation: School of Biomedical & Biological Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA, UK. Electronic address: aliaa.al-jubory@plymouth.ac.uk.
authors.forename: Aliaa R
authors.initials: AR
authors.lastname: Al-Jubory
authors.affiliation: School of Biomedical & Biological Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA, UK. Electronic address: R.Handy@plymouth.ac.uk.
authors.forename: Richard D
authors.initials: RD
authors.lastname: Handy
mesh_terms: D000584:Amiloride / Q000494:pharmacology; D018938:Caco-2 Cells; D002470:Cell Survival / Q000187:drug effects; D002746:Chlorpromazine / Q000494:pharmacology; D003460:Crystallization; D019833:Genistein / Q000494:pharmacology; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects / Q000648:ultrastructure; D053758:Nanoparticles / Q000378:metabolism*; D009761:Nystatin / Q000494:pharmacology; D010316:Particle Size; D014025:Titanium / Q000494:pharmacology*; D014638:Vanadates / Q000494:pharmacology
chemical_list: D009761:Nystatin; C009495:titanium dioxide; D014638:Vanadates; D000584:Amiloride; D014025:Titanium; D019833:Genistein; D002746:Chlorpromazine
keywords: Caco-2; Electrolyte homeostasis; Endocytosis; Titanium dioxide nanoparticles; Uptake mechanism