title: Mycobacterium tuberculosis-specific polyfunctional cytotoxic CD8+ T cells express CD69.
abstract: Increasing evidences in animals and humans suggest that CD8(+) T cells contribute significantly to immune defenses against Mycobacterium tuberculosis (Mtb). In the present study, we found that without any stimulation, CD8(+) T cells in pleural fluid cells (PFCs) expressed significantly higher levels of CD69 than PBMCs from patients with tuberculous pleurisy (TBP). CD8(+)CD69(+) T cells expressed significantly higher levels of CD45RO and HLA-DR and lower levels of CD45RA than CD8(+)CD69(-) T cells, demonstrating that CD8(+)CD69(+) T cells were activated memory cells. Furthermore, we found higher expression of CCR6 and lower expression of CCR7 and CD62L on CD8(+)CD69(+) T cells compared with CD8(+)CD69(-) T cells, suggesting that the expression of CCR6 and reduced expression of CCR7 and CD62L might facilitate the migration of circulating CD8(+)CD69(+) T cells into tuberculous pleural space. Importantly, following stimulation with culture filtrate protein of 10 kDa (CFP10) peptides, CD8(+)CD69(+) T cells but not CD8(+)CD69(-) T cells expressed CD107a/b, IFN-γ and TNF-α, demonstrating that CD8(+)CD69(+) T cells were MTB-specific cells. In addition, the majority of CD8(+)CD69(+) T cells were dominated by polyfunctional T cells. In summary, we demonstrated that CD69 as a useful marker for MTB-specific CD8(+) T cells in PFCs from patients with TBP enabled a direct ex vivo estimation of the quantity, as well as the quality, of MTB-specific CD8(+) responses.
authors.affiliation: Institute of Immunology, Zhongshan School of Medicine, Key Laboratory of Tropical Disease Control Research of Ministry of Education, Sun Yat-sen University, Guangzhou 510080, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Institute of Immunology, Zhongshan School of Medicine, Key Laboratory of Tropical Disease Control Research of Ministry of Education, Sun Yat-sen University, Guangzhou 510080, PR China.
authors.forename: Binyan
authors.initials: B
authors.lastname: Yang
authors.affiliation: Chest Hospital of Guangzhou, Guangzhou 510095, PR China.
authors.forename: Xianlan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Chest Hospital of Guangzhou, Guangzhou 510095, PR China.
authors.forename: Suihua
authors.initials: S
authors.lastname: Lao
authors.affiliation: Institute of Immunology, Zhongshan School of Medicine, Key Laboratory of Tropical Disease Control Research of Ministry of Education, Sun Yat-sen University, Guangzhou 510080, PR China. Electronic address: changyou_wu@yahoo.com.
authors.lastname: Changyou Wu
mesh_terms: D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000378:metabolism*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism*; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D037181:Lectins, C-Type / Q000378:metabolism*; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology; D014396:Tuberculosis, Pleural / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D055815:Young Adult; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D015415:Biomarkers; D007371:Interferon-gamma; D037181:Lectins, C-Type; D017493:Leukocyte Common Antigens; D014409:Tumor Necrosis Factor-alpha; D000099192:CD69 Antigens
keywords: CD69; CD8(+) T cells; Mycobacterium tuberculosis; Tuberculous pleurisy