title: The effects of fruiting positions on cellulose synthesis and sucrose metabolism during cotton (Gossypium hirsutum L.) fiber development.
abstract: Cotton (Gossypium hirsutum L.) boll positions on a fruiting branch vary in their contribution to yield and fiber quality. Fiber properties are dependent on deposition of cellulose in the fiber cell wall, but information about the enzymatic differences in sucrose metabolism between these fruiting positions is lacking. Therefore, two cotton cultivars with different sensitivities to low temperature were tested in 2010 and 2011 to quantify the effect of fruit positions (FPs) on fiber quality in relation to sucrose content, enzymatic activities and sucrose metabolism. The indices including sucrose content, sucrose transformation rate, cellulose content, and the activities of the key enzymes, sucrose phosphate synthase (SPS), acid invertase (AI) and sucrose synthase (SuSy) which inhibit cellulose synthesis and eventually affect fiber quality traits in cotton fiber, were determined. Results showed that as compared with those of FP1, cellulose content, sucrose content, and sucrose transformation rate of FP3 were all decreased, and the variations of cellulose content and sucrose transformation rate caused by FPs in Sumian 15 were larger than those in Kemian 1. Under FP effect, activities of SPS and AI in sucrose regulation were decreased, while SuSy activity in sucrose degradation was increased. The changes in activities of SuSy and SPS in response to FP effect displayed different and large change ranges between the two cultivars. These results indicate that restrained cellulose synthesis and sucrose metabolism in distal FPs are mainly attributed to the changes in the activities of these enzymes. The difference in fiber quality, cellulose synthesis and sucrose metabolism in response to FPs in fiber cells for the two cotton cultivars was mainly determined by the activities of both SuSy and SPS.
authors.affiliation: Key Laboratory of Crop Growth Regulation, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, Jiangsu Province, PR China.
authors.forename: Yina
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Key Laboratory of Crop Growth Regulation, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, Jiangsu Province, PR China.
authors.forename: Youhua
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Crop Growth Regulation, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, Jiangsu Province, PR China.
authors.forename: Jingran
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key Laboratory of Crop Growth Regulation, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, Jiangsu Province, PR China.
authors.forename: Fengjuan
authors.initials: F
authors.lastname: Lv
authors.affiliation: Key Laboratory of Crop Growth Regulation, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, Jiangsu Province, PR China.
authors.forename: Ji
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Laboratory of Crop Growth Regulation, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, Jiangsu Province, PR China.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D050260:Carbohydrate Metabolism; D002482:Cellulose / Q000378:metabolism*; D032561:Cotton Fiber; D035264:Flowers / Q000254:growth & development* / Q000378:metabolism; D005638:Fruit / Q000378:metabolism*; D005964:Glucosyltransferases / Q000378:metabolism*; D003368:Gossypium / Q000033:anatomy & histology / Q000254:growth & development* / Q000378:metabolism; D009929:Organ Size; D013395:Sucrose / Q000378:metabolism*; D043324:beta-Fructofuranosidase / Q000378:metabolism*
chemical_list: D013395:Sucrose; D002482:Cellulose; D005964:Glucosyltransferases; C022699:sucrose synthase; C022698:sucrose-phosphate synthase; D043324:beta-Fructofuranosidase