title: Enhanced intracellular siRNA delivery using bioreducible lipid-like nanoparticles.
abstract: A new library of lipid-like nanoparticles (lipidoids) comprising disulfide bond is developed for siRNA delivery. Bioreducible lipidoids deliver siRNA with greater efficiency than nonbioreducible lipidoids with similar chemical structures. A siRNA release investigation, as well as an intracellular siRNA trafficking study, reveals that the degradation of bioreducible lipidoid in a strongly reductive intracellular environment boosts siRNA release and enhances siRNA gene knockdown efficiency.
authors.affiliation: Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, MA, 02155, USA.
authors.forename: Ming
authors.initials: M
authors.lastname: Wang
authors.forename: Kyle
authors.initials: K
authors.lastname: Alberti
authors.forename: Antonio
authors.initials: A
authors.lastname: Varone
authors.forename: Dimitria
authors.initials: D
authors.lastname: Pouli
authors.forename: Irene
authors.initials: I
authors.lastname: Georgakoudi
authors.forename: Qiaobing
authors.initials: Q
authors.lastname: Xu
mesh_terms: D045744:Cell Line, Tumor; D055785:Gene Knockdown Techniques / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D042541:Intracellular Space / Q000737:chemistry / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry / Q000493:pharmacokinetics*; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014162:Transfection / Q000379:methods
chemical_list: D008055:Lipids; D034741:RNA, Small Interfering; D049452:Green Fluorescent Proteins
keywords: bioreducible lipidoid; cancer therapy; combinatorial; nanoparticles; siRNA delivery