title: Fructose-1,6-bisphosphatase, Malate Dehydrogenase, Isocitrate Lyase, Phosphoenolpyruvate Carboxykinase, Glyceraldehyde-3-phosphate Dehydrogenase, and Cyclophilin A are secreted in Saccharomyces cerevisiae grown in low glucose.
abstract: Our previous studies demonstrated that the key gluconeogenic enzyme fructose-1,6-bisphosphatase is secreted when Saccharomyces cerevisiae are starved of glucose for a prolonged period of time. In this study, we showed that malate dehydrogenase, isocitrate lyase, phosphoenolpyruvate carboxykinase, glyceraldehyde-3-phosphate dehydrogenase, and cyclophilin A are also secreted in glucose-starved cells. Thus, both gluconeogenic and non-gluconeogenic enzymes are secreted via the non-classical pathway.
authors.affiliation: Department of Cellular and Molecular Physiology; Penn State University College of Medicine; Hershey, PA USA.
authors.forename: Bennett J
authors.initials: BJ
authors.lastname: Giardina
authors.affiliation: Department of Cellular and Molecular Physiology; Penn State University College of Medicine; Hershey, PA USA.
authors.forename: Hui-Ling
authors.initials: HL
authors.lastname: Chiang
keywords: extracellular proteins; fructose-1,6-bisphosphatase; gluconeogenesis; malate dehydrogenase; non-classical secretion; periplasm; phosphoenolpyruvate carboxykinase; vacuole; vacuole import and degradation