title: Ribonuclease binase decreases destructive changes of the liver and restores its regeneration potential in mouse lung carcinoma model.
abstract: The successful application of exogenous ribonucleases of different origin to suppress tumor growth allows one to consider them as perspective therapeutics for treatment of oncological diseases. An important aspect of the success of an anti-cancer drug is low hepatotoxicity, which will reduce, if not eliminate entirely the undesirable side effects of treatment. Previously we have shown that ribonuclease from Bacillus intermedius (binase) exhibits high antitumor and antimetastatic activity in tumor models of different histological origin. In this work we studied hepatotoxic action of binase using mouse tumor model of Lewis lung carcinoma. Binase at doses of 0.1-1 mg/kg which produced effective suppression of tumor growth and metastasis, showed positive effect on the liver of tumor-bearing mice expressed in a significant reduction in the volume of destructive changes in the liver parenchyma and return to the normal level of the liver regenerative potential impaired due to endogenous intoxication and tumor burden.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine SB RAS, 8, Lavrentiev Ave., Novosibirsk, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Sen'kova
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine SB RAS, 8, Lavrentiev Ave., Novosibirsk, Russia. Electronic address: mironova@niboch.nsc.ru.
authors.forename: N L
authors.initials: NL
authors.lastname: Mironova
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine SB RAS, 8, Lavrentiev Ave., Novosibirsk, Russia.
authors.forename: O A
authors.initials: OA
authors.lastname: Patutina
authors.affiliation: Engelhardt Institute of Molecular Biology RAS, Vavilov Str. 32, 119991 Moscow, Russia.
authors.forename: V A
authors.initials: VA
authors.lastname: Mitkevich
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine SB RAS, 8, Lavrentiev Ave., Novosibirsk, Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Markov
authors.affiliation: Engelhardt Institute of Molecular Biology RAS, Vavilov Str. 32, 119991 Moscow, Russia.
authors.forename: I Y
authors.initials: IY
authors.lastname: Petrushanko
authors.affiliation: Engelhardt Institute of Molecular Biology RAS, Vavilov Str. 32, 119991 Moscow, Russia.
authors.forename: K M
authors.initials: KM
authors.lastname: Burnysheva
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine SB RAS, 8, Lavrentiev Ave., Novosibirsk, Russia.
authors.forename: M A
authors.initials: MA
authors.lastname: Zenkova
authors.affiliation: Engelhardt Institute of Molecular Biology RAS, Vavilov Str. 32, 119991 Moscow, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Makarov
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000633:toxicity; D018827:Carcinoma, Lewis Lung / Q000188:drug therapy* / Q000473:pathology; D004722:Endoribonucleases / Q000494:pharmacology* / Q000633:toxicity; D005260:Female; D008099:Liver / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D008115:Liver Regeneration / Q000187:drug effects*; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000556:secondary; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D047368:Tumor Burden / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D004722:Endoribonucleases; C022543:ribonuclease T(2)
keywords: Cancer; Destructive changes of the liver; Murine tumor model; RNase; Regeneration potential of the liver