title: Activity-based proteomic and metabolomic approaches for understanding metabolism.
abstract: There are an increasing number of human pathologies that have been associated with altered metabolism, including obesity, diabetes, atherosclerosis, cancer, and neurodegenerative diseases. Most attention on metabolism has been focused on well-understood metabolic pathways and has largely ignored most of the biochemical pathways that operate in (patho)physiological settings, in part because of the vast landscape of uncharacterized and undiscovered metabolic pathways. One technology that has arisen to meet this challenge is activity-based protein profiling (ABPP) that uses activity-based chemical probes to broadly assess the functional states of both characterized and uncharacterized enzymes. This review will focus on how ABPP, coupled with inhibitor discovery platforms and functional metabolomic technologies, have led to discoveries that have expanded our knowledge of metabolism in health and disease.
authors.affiliation: Program in Metabolic Biology, University of California, Berkeley, Berkeley, CA 94720, United States.
authors.forename: Devon
authors.initials: D
authors.lastname: Hunerdosse
authors.affiliation: Program in Metabolic Biology, University of California, Berkeley, Berkeley, CA 94720, United States. Electronic address: dnomura@berkeley.edu.
authors.forename: Daniel K
authors.initials: DK
authors.lastname: Nomura
mesh_terms: D000818:Animals; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D053858:Metabolic Networks and Pathways; D055432:Metabolomics; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology; D011485:Protein Binding; D025941:Protein Interaction Mapping; D020543:Proteome / Q000032:analysis; D040901:Proteomics
chemical_list: D004791:Enzyme Inhibitors; D004798:Enzymes; D020543:Proteome