title: Profiling of RNA modifications by multiplexed stable isotope labelling.
abstract: The combination of (15)N/(13)C stable isotope labelling (SIL) and LC-MS/MS revealed a total of 52 modifications in RNA from E. coli and yeast, including 10 previously undescribed modifications. Two modifications, N-ribosylnicotinamide and 2-methylthioadenosine, were newly detected in species hitherto thought not to contain these modifications.
authors.affiliation: Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, 5 Staudinger Weg, Mainz, D-55128, Germany. mhelm@uni-mainz.de.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Kellner
authors.forename: Jennifer
authors.initials: J
authors.lastname: Neumann
authors.forename: David
authors.initials: D
authors.lastname: Rosenkranz
authors.forename: Svetlana
authors.initials: S
authors.lastname: Lebedeva
authors.forename: René F
authors.initials: RF
authors.lastname: Ketting
authors.forename: Hans
authors.initials: H
authors.lastname: Zischler
authors.forename: Dirk
authors.initials: D
authors.lastname: Schneider
authors.forename: Mark
authors.initials: M
authors.lastname: Helm
mesh_terms: D002853:Chromatography, Liquid; D004926:Escherichia coli / Q000235:genetics; D007553:Isotope Labeling; D012313:RNA / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D053719:Tandem Mass Spectrometry
chemical_list: D012313:RNA