title: [Effects of stanniocalcin-1 and hypoxia-inducible factor-1α on mitochondrial membrane potential stability in renal carcinoma cells].
abstract: OBJECTIVE
  To explore the effects of stanniocalcin-1 (STC-1) and hypoxia-inducible factor-1α (HIF-1α) on the calcium and thus on the mitochondrial membrane potential (Δψm) in renal carcinoma cells.
  
  
  METHODS
  We successfully established the renal carcinoma cell models with high HIF-1α gene expression. After various concentrations of STC-1 solutions were added to the culture medium, the proliferation of cells, expressions of HIF-1α and STC-1, levels of Ca(2+), Δψm, and mPTP were detected by MTT, RT-PCR, ELISA, fluorescence spectrophotometry, and ultraviolet spectrophotometry, respectively.
  
  
  RESULTS
  The proliferation of renal carcinoma cells and Δψm were improved after HIF-1α gene transfection, STC-1 protein intervention, and STC-1 protein intervention after gene transfection. While the intracellular Ca(2+) level and mPTP were decreased significantly (P<0.05), all the changes were intensified with the gradual increase of STC-1. However, the increasing trend of cell proliferation gradually declined.
  
  
  CONCLUSIONS
  HIF-1α may participate in malignant proliferation of renal carcinoma cells by promoting STC-1 proliferation or down-regulating Ca(2+); however, such an effect may be gradually attenuated due to the inhibitory effect of STC-1 on HIF-1α.
authors.affiliation: Department of Urologic Surgery, Affiliated Hospital, Guiyang Medical College, Guiyang 550004, China.
authors.forename: Qing-tao
authors.initials: QT
authors.lastname: Yang
authors.affiliation: Department of Urologic Surgery, Affiliated Hospital, Guiyang Medical College, Guiyang 550004, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Gu
authors.affiliation: Department of Urologic Surgery, Affiliated Hospital, Guiyang Medical College, Guiyang 550004, China.
authors.forename: Yong-chun
authors.initials: YC
authors.lastname: Zhang
authors.affiliation: Department of Urologic Surgery, Affiliated Hospital, Guiyang Medical College, Guiyang 550004, China.
authors.forename: Zhi-hui
authors.initials: ZH
authors.lastname: Zhu
authors.affiliation: Department of Urologic Surgery, Affiliated Hospital, Guiyang Medical College, Guiyang 550004, China.
authors.forename: Yong-an
authors.initials: YA
authors.lastname: Yang
authors.affiliation: Department of Urologic Surgery, Affiliated Hospital, Guiyang Medical College, Guiyang 550004, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.affiliation: Department of Urologic Surgery, Jiangdu People's Hospital, Yangzhou, Jiangsu 225200, China.
authors.forename: Qing-liang
authors.initials: QL
authors.lastname: Zhu
mesh_terms: D002118:Calcium / Q000378:metabolism; D002292:Carcinoma, Renal Cell / Q000473:pathology*; D006023:Glycoproteins / Q000494:pharmacology*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D014407:Tumor Cells, Cultured
chemical_list: D006023:Glycoproteins; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; C051795:teleocalcin; D002118:Calcium