title: Plasma biomarkers profile of female dogs with mammary carcinoma and its association with clinical and pathological features.
abstract: The immunological biomarkers profiles were evaluated using Luminex as putative measures to monitor canine mammary carcinomas (MCs). Forty female dogs were categorized into benign mixed tumour (MC-BMT = 28) and mammary carcinoma (MC=12). The ascendant biomarker signatures were used to compare the groups. For example, a higher frequency of MC-BMT animals producing IL-6, CXCL-8 and CXCL-10 was observed, whereas for the MC group IL-2 and CXCL-8 were detected. MC-BMT animals without metastasis had an increase in the levels of IL-2, CXCL-8, CXCL-10, IL-6, TNF-α, IL-15 and a decrease in IL-10 and CXCL-8. MC-BMT animals with metastasis showed only an increase in CXCL-10 and a decrease in IL-18. After comparing the ascendant signatures following the presence of metastasis in both groups, a higher frequency of dogs exhibiting IL-10 production was observed. Pearson correlation (P = 0.0273) and receiver operating characteristic (ROC) curve analysis revealed that this pattern was associated with worse outcome and lower survival rates in MC animals.
authors.affiliation: Departamento de Patologia e Clínica, Escola de Medicina Veterinária, Universidade Federal da Bahia, Salvador, BA, Brazil.|Laboratório de Patologia Comparada (LPC), Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: A
authors.initials: A
authors.lastname: Estrela-Lima
authors.affiliation: Laboratório de Biomarcadores de Diagnóstico e Monitoração, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, MG, Brazil.
authors.forename: M S S
authors.initials: MS
authors.lastname: Araújo
authors.affiliation: Laboratório de Biomarcadores de Diagnóstico e Monitoração, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, MG, Brazil.
authors.forename: R P
authors.initials: RP
authors.lastname: Soares
authors.affiliation: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária da Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: L G R
authors.initials: LG
authors.lastname: Ribeiro
authors.affiliation: Laboratório de Patologia Comparada (LPC), Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: K A
authors.initials: KA
authors.lastname: Damasceno
authors.affiliation: Departamento de Patologia e Clínica, Escola de Medicina Veterinária, Universidade Federal da Bahia, Salvador, BA, Brazil.
authors.forename: A T
authors.initials: AT
authors.lastname: Costa
authors.affiliation: Laboratório de Biomarcadores de Diagnóstico e Monitoração, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, MG, Brazil.
authors.forename: A
authors.initials: A
authors.lastname: Teixeira-Carvalho
authors.affiliation: Laboratório de Biomarcadores de Diagnóstico e Monitoração, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, MG, Brazil.
authors.forename: O A
authors.initials: OA
authors.lastname: Martins-Filho
authors.affiliation: Laboratório de Patologia Comparada (LPC), Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: G D
authors.initials: GD
authors.lastname: Cassali
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood*; D002277:Carcinoma / Q000097:blood / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D016207:Cytokines / Q000097:blood / Q000235:genetics / Q000378:metabolism; D004283:Dog Diseases / Q000097:blood* / Q000378:metabolism / Q000473:pathology; D004285:Dogs; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D015674:Mammary Neoplasms, Animal / Q000097:blood* / Q000378:metabolism / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D016207:Cytokines
keywords: clinical pathology; comparative oncology; immunology; metastasis; oncology