title: Identification of key regulators in glycogen utilization in E. coli based on the simulations from a hybrid functional Petri net model.
abstract: BACKGROUND
  Glycogen and glucose are two sugar sources available during the lag phase of E. coli, but the mechanism that regulates their utilization is still unclear.
  
  
  METHODS
  Attempting to unveil the relationship between glucose and glycogen, we propose an integrated hybrid functional Petri net (HFPN) model including glycolysis, PTS, glycogen metabolic pathway, and their internal regulatory systems.
  
  
  CONCLUSIONS
  By comparing known biological results to this model, basic necessary regulatory mechanism for utilizing glucose and glycogen were identified as a feedback circuit in which HPr and EIIAGlc play key roles. Based on this regulatory HFPN model, we discuss the process of glycogen utilization in E. coli in the context of a systematic understanding of carbohydrate metabolism.
authors.forename: Zhongyuan
authors.initials: Z
authors.lastname: Tian
authors.forename: Adrien
authors.initials: A
authors.lastname: Fauré
authors.forename: Hirotada
authors.initials: H
authors.lastname: Mori
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Matsuno
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism*; D008954:Models, Biological; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism; D021381:Protein Transport
chemical_list: D001426:Bacterial Proteins; D006003:Glycogen; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C030182:phosphocarrier protein HPr; D005947:Glucose