title: A novel combination of plant growth regulators for in vitro regeneration of complete plantlets of guar [Cyamopsis tetragonoloba (L.) Taub].
abstract: A novel combination of plant growth regulators comprising indole-3-butyric acid (IBA), 6-benzylaminopurine (BA) and gibberellic acid (GA3) in Murashige and Skoog basal medium has been formulated for in vitro induction of both shoot and root in one culture using cotyledonary node explants of guar, (Cyamopsis tetragonoloba). Highest percentages of shoot (92%) and root (80%) induction were obtained in the medium containing (mg/L) 2 IBA, 3 BA and 1 GA3. Shoot regeneration from the cotyledonary node explants was observed after 10-15 days. Regeneration of roots from these shoots occurred after 20 to 25 days. The regenerated plantlets showed successful acclimatization on transfer to soil. This protocol is expected to be helpful in carrying out various in vitro manipulations in this economically and industrially important legume.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, India.
authors.forename: S
authors.initials: S
authors.lastname: Verma
authors.affiliation: Department of Crop and Soil Science, Washington State University, P O Box: 646420, Johnson Hall, 277, Pullman, WA 99164-6420, USA.
authors.forename: K S
authors.initials: KS
authors.lastname: Gill
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, India.
authors.forename: V
authors.initials: V
authors.lastname: Pruthi
authors.affiliation: DuPont Agricultural Biotechnology, DuPont Pioneer, 7300 NW 62nd Avenue, Johnston, IA 50131, USA.
authors.forename: K S
authors.initials: KS
authors.lastname: Dhugga
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, India.
authors.forename: G S
authors.initials: GS
authors.lastname: Randhaw
mesh_terms: D001593:Benzyl Compounds; D031293:Cyamopsis / Q000187:drug effects / Q000254:growth & development*; D005875:Gibberellins / Q000494:pharmacology; D007211:Indoles / Q000494:pharmacology; D007701:Kinetin / Q000494:pharmacology; D063245:Plant Development / Q000187:drug effects*; D010937:Plant Growth Regulators / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development; D011687:Purines
chemical_list: D001593:Benzyl Compounds; D005875:Gibberellins; D007211:Indoles; D010937:Plant Growth Regulators; D011687:Purines; C014612:indolebutyric acid; C007842:gibberellic acid; C480551:benzylaminopurine; D007701:Kinetin