title: Residual HEMA and TEGDMA release and cytotoxicity evaluation of resin-modified glass ionomer cement and compomers cured with different light sources.
abstract: The purpose of this study was first to evaluate the elution of 2-hydroxyethyl methacrylate (HEMA) and triethylene glycol dimethacrylate (TEGDMA) monomers from resin-modified glass ionomer cement (RMGIC) and compomers cured with halogen and light-emitting diode (LED) light-curing units (LCUs). The effect of cured materials on the viability of L929 fibroblast cells was also evaluated. One RMGIC (Ketac N100) and two compomers (Dyract Extra and Twinkystar) were tested. Materials were prepared in teflon disks and light-cured with LED or halogen LCUs. The residual monomers of resin materials in solution were identified using high-performance liquid chromatography. The fibroblast cells' viability was analyzed using MTT assay. The type of LCU did not have a significant effect on the elution of HEMA and TEGDMA. A greater amount of HEMA than TEGMDA was eluted. The amount of TEGDMA eluted from Twinkystar was greater than Dyract Extra (P < 0.05) when cured with a halogen LCU. All material-LCU combinations decreased the fibroblast cells' viability more than the control group (P < 0.01), except for Dyract Extra cured with a halogen LCU (P > 0.05). Curing with the LED LCU decreased the cells' viability more than curing with the halogen LCU for compomers. For Ketac N100, the halogen LCU decreased the cells' viability more than the LED LCU.
authors.affiliation: Department of Pedodontics, Faculty of Dentistry, Selcuk University, Selcuklu 42079, Konya, Turkey.
authors.forename: Murat Selim
authors.initials: MS
authors.lastname: Botsali
authors.affiliation: Department of Pedodontics, Faculty of Dentistry, Karadeniz Technical University, 61080 Trabzon, Turkey.
authors.forename: Adem
authors.initials: A
authors.lastname: Kuşgöz
authors.affiliation: Department of Prosthodontics, Faculty of Dentistry, Karadeniz Technical University, 61080 Trabzon, Turkey.
authors.forename: Subutay Han
authors.initials: SH
authors.lastname: Altintaş
authors.affiliation: Department of Restorative Dentistry, Faculty of Dentistry, Selcuk University, 42079 Konya, Turkey.
authors.forename: Hayriye Esra
authors.initials: HE
authors.lastname: Ülker
authors.affiliation: Department of Pedodontics, Faculty of Dentistry, Şifa University, 35535 İzmir, Turkey.
authors.forename: Mehmet
authors.identifier: 0000-0003-0500-293X
authors.initials: M
authors.lastname: Tanriver
authors.affiliation: Department of Prosthodontics, Faculty of Dentistry, Karadeniz Technical University, 61080 Trabzon, Turkey.
authors.forename: Serdar
authors.initials: S
authors.lastname: Kiliç
authors.affiliation: Department of Pedodontics, Centre for Dental Sciences, Gulhane Military Medical Academy, 06010 Ankara, Turkey.
authors.forename: Feridun
authors.initials: F
authors.lastname: Başak
authors.affiliation: Department of Restorative Dentistry, Faculty of Dentistry, Selcuk University, 42079 Konya, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ülker
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival; D055117:Curing Lights, Dental / Q000009:adverse effects*; D003738:Dental Cements / Q000737:chemistry / Q000528:radiation effects / Q000633:toxicity*; D005347:Fibroblasts / Q000187:drug effects*; D008027:Light / Q000009:adverse effects; D008689:Methacrylates / Q000737:chemistry / Q000528:radiation effects / Q000633:toxicity*; D051379:Mice; D011092:Polyethylene Glycols / Q000737:chemistry / Q000528:radiation effects / Q000633:toxicity*; D011109:Polymethacrylic Acids / Q000737:chemistry / Q000528:radiation effects / Q000633:toxicity*
chemical_list: D003738:Dental Cements; D008689:Methacrylates; D011109:Polymethacrylic Acids; C020946:triethylene glycol dimethacrylate; D011092:Polyethylene Glycols; C005044:hydroxyethyl methacrylate