title: Deciphering the stromal and hematopoietic cell network of the adventitia from non-aneurysmal and aneurysmal human aorta.
abstract: Aneurysm is associated to a complex remodeling of arteries that affects all their layers. Although events taking place in the intima and the media have received a particular attention, molecular and cellular events taking place in the adventitia have started to be deciphered only recently. In this study, we have precisely described the composition and distribution of stromal and hematopoietic cells in human arterial adventitia, both at steady state and in the setting of aortic aneurysm. Using polychromatic immunofluorescent and flow cytometry analyses, we observed that unlike the medial layer (which comprises mostly macrophages and T cells among leukocytes), the adventitia comprises a much greater variety of leukocytes. We observed an altered balance in macrophages subsets in favor of M2-like macrophages, an increased proliferation of macrophages, a greater number of all stromal cells in aneurysmal aortas. We also confirmed that in this pathological setting, adventitia comprised blood vessels and arterial tertiary lymphoid organs (ATLOs), which contained also M-DC8(+) dendritic cells (slanDCs) that could participate in the induction of T-cell responses. Finally, we showed that lymphatic vessels can be detected in aneurysmal adventitia, the functionality of which will have to be evaluated in future studies. All together, these observations provide an integrative outlook of the stromal and hematopoietic cell network of the human adventitia both at steady state and in the context of aneurysm.
authors.affiliation: INSERM (Institut National de la Santé et de la Recherche Médicale) U698, Hemostasis, Bio-engineering and Cardiovascular Remodeling, Hôpital Bichat, Paris, France.
authors.forename: Charles-Antoine
authors.initials: CA
authors.lastname: Dutertre
authors.affiliation: INSERM (Institut National de la Santé et de la Recherche Médicale) U698, Hemostasis, Bio-engineering and Cardiovascular Remodeling, Hôpital Bichat, Paris, France ; Université Denis Diderot, Paris, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Clement
authors.affiliation: INSERM (Institut National de la Santé et de la Recherche Médicale) U698, Hemostasis, Bio-engineering and Cardiovascular Remodeling, Hôpital Bichat, Paris, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Morvan
authors.affiliation: Department of Dermatology, University Hospital Heidelberg, Germany.
authors.forename: Knut
authors.initials: K
authors.lastname: Schäkel
authors.affiliation: Université Denis Diderot, Paris, France ; AP-HP, Service de chirurgie Vasculaire et Thoracique, Hôpital Bichat, Paris, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Castier
authors.affiliation: AP-HP, Service de chirurgie cardiaque et vasculaire, Hopital Européen Georges Pompidou, Faculté de Médecine René Descartes, Université Paris 5, Paris, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Alsac
authors.affiliation: INSERM (Institut National de la Santé et de la Recherche Médicale) U698, Hemostasis, Bio-engineering and Cardiovascular Remodeling, Hôpital Bichat, Paris, France ; Université Denis Diderot, Paris, France ; DHU Fire, Paris, France.
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Michel
authors.affiliation: INSERM (Institut National de la Santé et de la Recherche Médicale) U698, Hemostasis, Bio-engineering and Cardiovascular Remodeling, Hôpital Bichat, Paris, France ; Université Denis Diderot, Paris, France ; DHU Fire, Paris, France.
authors.forename: Antonino
authors.initials: A
authors.lastname: Nicoletti
mesh_terms: D063194:Adventitia / Q000166:cytology* / Q000378:metabolism; D001014:Aortic Aneurysm / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D016130:Immunophenotyping; D007962:Leukocytes / Q000378:metabolism; D017154:Stromal Cells / Q000378:metabolism