title: Microarray detection of human papilloma virus genotypes among Turkish women with abnormal cytology at a colposcopy unit.
abstract: OBJECTIVE
  There is a well-known association between human papilloma virus (HPV) and cervical neoplasia. The aim of this study was to investigate the types of HPV DNA and to compare the results with colposcopic findings among women with abnormal cytology.
  
  
  METHODS
  A series of 76 consecutive women attending the clinic with the usual referral indications (ASC-US or higher in Pap) were examined by the conventional diagnostic tools (PAP smear, colposcopy,punch biopsy) and subjected to HPV testing. For HPV genotyping, we used a commercially avaliable HPV DNA chip (Genomica-CLART) which is a PCR based microarray system.The HPV test detected 35types of HPV (HPV-6/-11/-16/-18/-26/-31/-33/-35/-39/-40/-42/-43/-44/-45/-51/-52/-53/-54/-56/-58/-59/-61/-62/-66/-70/-71/-72/-73/-81/-83/84/-85/-89).
  
  
  RESULTS
  Overall, 44.7% of all patients were HPV positive. HPV was positive in 35%, 51.9%, 77.7% of the ASCUS, LSIL and HSIL groups respectively and HPV 16 was the most prevalent type in all groups. 6 %of patients had mutiple infections. 57.8% of biopsy proven SIL's were HPV positive. The most prevalent HPV type was HPV 16 (54.5%).Colposcopic assessment revealed pathologic findings in 94.7% of biopsy proven SIL cases.
  
  
  CONCLUSIONS
  Although it has been reported that the prevalence of HPV in the general population is lower than Western countries, and the prevalence and distribution of genotypes are smilar in patients with abnormal cytology. Further population based studies are needed to determine the prevalance and type distribution of HPV with normal and abnormal cytology in Turkish women. Despite the new technological progress in HPV virion, colposcopy is still very important diagnostic tool in the management of abnormal smears.
authors.affiliation: Department of Obstetrics and Gynecology, İstanbul University, İstanbul Medical School, İstanbul, Turkey.
authors.forename: Işıl Uzun
authors.initials: IU
authors.lastname: Cilingir
authors.affiliation: Department of Obstetrics and Gynecology, İstanbul University, İstanbul Medical School, İstanbul, Turkey.
authors.forename: Ergin
authors.initials: E
authors.lastname: Bengisu
authors.affiliation: Department of Microbiology, İstanbul University, İstanbul Medical School,İstanbul, Turkey.
authors.forename: Ali
authors.initials: A
authors.lastname: Ağaçfidan
authors.affiliation: Department of Microbiology, İstanbul University, İstanbul Medical School,İstanbul, Turkey.
authors.forename: Muammer Osman
authors.initials: MO
authors.lastname: Koksal
authors.affiliation: Department of Obstetrics and Gynecology, İstanbul University, İstanbul Medical School, İstanbul, Turkey.
authors.forename: Samet
authors.initials: S
authors.lastname: Topuz
authors.affiliation: Department of Obstetrics and Gynecology, İstanbul University, İstanbul Medical School, İstanbul, Turkey.
authors.forename: Sinan
authors.initials: S
authors.lastname: Berkman
authors.affiliation: Department of Obstetrics and Gynecology, İstanbul University, İstanbul Medical School, İstanbul, Turkey.
authors.forename: Ahmet Cem
authors.initials: AC
authors.lastname: Iyibozkurt
keywords: Colposcopy; HPV; Microarray; PCR; abnormal smear