title: Immunoblotting analyses of changes in protein phosphorylations during oocyte maturation in marine nemertean worms.
abstract: Immunoblotting analyses combined with phospho-specific antibodies can provide a powerful means for assessing protein activity states in various cellular extracts. This chapter describes a traditional, film-based immunoblotting method for monitoring the phosphorylation status of proteins in marine nemertean oocytes undergoing maturation. Similarly, with minor modifications, the protocol could potentially be applied to a wider variety of cellular processes and extract types that might be analyzed in other investigations of marine invertebrate development.
authors.affiliation: Department of Biology, University of New Mexico, Albuquerque, NM, USA.
authors.forename: Jose R
authors.initials: JR
authors.lastname: Escalona
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Stricker
mesh_terms: D000818:Animals; D059001:Aquatic Organisms / Q000166:cytology; D015153:Blotting, Western; D007448:Invertebrates / Q000166:cytology; D009865:Oocytes / Q000201:enzymology* / Q000378:metabolism; D009866:Oogenesis; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D011499:Protein Processing, Post-Translational
chemical_list: D011494:Protein Kinases