title: Changes in the bacterial community structure in stored wormbed leachate.
abstract: Organic wastes, such as cow manure, are often composted with earthworms (vermicomposting) while excess water is drained and collected. This wormbed leachate is nutrient-rich and it has been extensively used to fertilize plants. However, it is derived partially from a not yet finished compost process and could exhibit phytotoxicity or contain potentially hazardous microorganisms. The bacterial community in wormbed leachate derived from vermicomposting of cow manure was studied by pyrosequencing the 16S rRNA gene. The fresh wormbed leachate was rich in Mollicutes, particularly the genus Acholeplasma which contain phytopathogen species. The abundance of the Mollicutes decreased when the leachate was stored, while that of the Rhizobiales and the genus Pseudomonas increased. The bacterial communities changed rapidly in the leachate during storage. The changes in ammonium, nitrate and inorganic carbon content of the wormbed leachate when stored were correlated to changes in the bacterial community structure. It was found that storage of the wormbed leachate might be required before it can be applied to crops as large proportions of potentially plant pathogens were found in the fresh leachate.
authors.affiliation: Laboratory of Soil Ecology, ABACUS, Cinvestav, Mexico D.F. Mexico.
authors.forename: Elda M
authors.initials: EM
authors.lastname: Romero-Tepal
authors.forename: Eduardo
authors.initials: E
authors.lastname: Contreras-Blancas
authors.forename: Yendi E
authors.initials: YE
authors.lastname: Navarro-Noya
authors.forename: Víctor M
authors.initials: VM
authors.lastname: Ruíz-Valdiviezo
authors.forename: Marco
authors.initials: M
authors.lastname: Luna-Guido
authors.forename: Federico A
authors.initials: FA
authors.lastname: Gutiérrez-Miceli
authors.forename: Luc
authors.initials: L
authors.lastname: Dendooven
mesh_terms: D064751:Ammonium Compounds / Q000032:analysis; D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000235:genetics*; D058448:Biota; D017554:Carbon Compounds, Inorganic / Q000032:analysis; D002417:Cattle; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008372:Manure; D009566:Nitrates / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology
chemical_list: D064751:Ammonium Compounds; D017554:Carbon Compounds, Inorganic; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D008372:Manure; D009566:Nitrates; D012336:RNA, Ribosomal, 16S; D012987:Soil