title: The Notch co-repressor protein NKAP is highly expressed in adult mouse subventricular zone neural progenitor cells.
abstract: In the adult mammalian brain niches for neural stem cells are maintained, which enable a steady-state neurogenesis. This process is tightly regulated by multiple niche factors, including Notch and NF-κB signaling. The NF-κB-activating-protein (NKAP) has previously been shown to act as Notch co-repressor component by binding CIR and recruiting HDAC3 in T-cell development and furthermore to regulate NF-κB-dependent transcription. Here, we provide first evidence for the expression of NKAP in neurogenic cells of the adult mammalian brain. NKAP is highly expressed in Mash1(+) transit amplifying cells and PSA-NCAM(+) migrating neuroblasts throughout the subventricular zone (SVZ) and the rostral migratory stream (RMS), as well as in the hippocampus. We further show that NKAP expression levels are downregulated during the course of the RMS. Eventually, most differentiated cells in the olfactory bulb (OB) and the corpus callosum only display low levels of NKAP expression. Finally, large subsets of mature neurons in the OB, the hippocampus and the thalamus express NKAP at high levels, suggesting an additional role of NKAP outside of SVZ progenitor cells.
authors.affiliation: Westfälische Wilhelms-Universität Münster, ZMBE, Institute of Cell Biology, Stem Cell Biology and Regeneration Group, Von-Esmarch-Straße 56, 48149 Münster, Germany; Interdisciplinary Center for Clinical Research (IZKF) Münster, Germany.
authors.forename: M M A
authors.initials: MM
authors.lastname: Worlitzer
authors.affiliation: Westfälische Wilhelms-Universität Münster, ZMBE, Institute of Cell Biology, Stem Cell Biology and Regeneration Group, Von-Esmarch-Straße 56, 48149 Münster, Germany; Interdisciplinary Center for Clinical Research (IZKF) Münster, Germany; Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Esch-Belval, Luxembourg. Electronic address: jschwamb@uni-muenster.de.
authors.forename: J C
authors.initials: JC
authors.lastname: Schwamborn
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation; D005455:Fluorescent Antibody Technique; D057809:HEK293 Cells; D006801:Humans; D020547:Lateral Ventricles / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D012097:Repressor Proteins / Q000096:biosynthesis*
chemical_list: C560431:NKAP protein, mouse; D012097:Repressor Proteins
keywords: NF-κB signaling; NKAP; adult neurogenesis; mature neurons; notch signaling