title: Detection and localization of rabbit hepatitis e virus and antigen in systemic tissues from experimentally intraperitoneally infected rabbits.
abstract: Rabbit hepatitis E virus (HEV) is a novel genotype of HEV, and is considered to pose a risk of zoonotic transmission. Research into the systemic distribution of rabbit HEV in rabbits during different periods of infection has rarely been reported. To better understand this virus, we infected rabbits with second-passage rabbit HEV via an intraperitoneal route. After inoculation, the infection showed two types, temporary and constant infection. The detection of HEV RNA in the feces varied with time, and serum antigen correlated with fecal HEV RNA. Viremia only appeared 72 days after inoculation. The rabbits remained antibody negative throughout the experimental period. When HEV was localized, several organs besides the liver were HEV RNA positive. Tissue antigen was observed immunohistochemically in the different cells of various organs, especially in parts of the small intestine and the characteristic rabbit gut-associated lymphoid tissue. These data provide valuable information for future research into the pathogenesis of HEV.
authors.affiliation: Department of Veterinary Pathology, Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Key Laboratory of Zoonosis of Ministry of Agriculture, Beijing, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Mao
authors.affiliation: Department of Veterinary Pathology, Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Key Laboratory of Zoonosis of Ministry of Agriculture, Beijing, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Veterinary Pathology, Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Key Laboratory of Zoonosis of Ministry of Agriculture, Beijing, China.
authors.forename: Ruiping
authors.initials: R
authors.lastname: She
authors.affiliation: Department of Veterinary Pathology, Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Key Laboratory of Zoonosis of Ministry of Agriculture, Beijing, China.
authors.forename: Binbin
authors.initials: B
authors.lastname: Cao
authors.affiliation: Department of Veterinary Pathology, Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Key Laboratory of Zoonosis of Ministry of Agriculture, Beijing, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xiao
authors.affiliation: Department of Veterinary Pathology, Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Key Laboratory of Zoonosis of Ministry of Agriculture, Beijing, China.
authors.forename: Qiaoxing
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Veterinary Pathology, Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Key Laboratory of Zoonosis of Ministry of Agriculture, Beijing, China.
authors.forename: Zhaojie
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Department of Veterinary Pathology, Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Key Laboratory of Zoonosis of Ministry of Agriculture, Beijing, China.
authors.forename: Longhuan
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Veterinary Pathology, Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Key Laboratory of Zoonosis of Ministry of Agriculture, Beijing, China.
authors.forename: Majid Hussain
authors.initials: MH
authors.lastname: Soomro
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000821:virology; D018963:Hepatitis Antigens / Q000276:immunology*; D016751:Hepatitis E / Q000097:blood / Q000276:immunology* / Q000821:virology*; D016752:Hepatitis E virus / Q000276:immunology*; D007150:Immunohistochemistry; D007274:Injections, Intraperitoneal; D009928:Organ Specificity / Q000276:immunology*; D012367:RNA, Viral / Q000097:blood; D011817:Rabbits
chemical_list: D018963:Hepatitis Antigens; D012367:RNA, Viral