title: Serotype-associated polymorphisms in a partial rpoB gene sequence of Salmonella enterica.
abstract: Salmonella enterica is a zoonotic bacterium with more than 2500 serotypes, which affect a wide range of hosts and produce diverse clinical outcomes. Strain identification usually involves costly and time-demanding procedures. This paper describes the sequencing of a rpoB hypervariable gene segment (847 bp) that allows identification of serotypes in S. enterica strains isolated from several hosts. The nucleotide similarity values among S. enterica serotypes ranged from 98.23% to 99.88%, with potential usefulness for devising a simple one-step sequencing as a first approach for identification of S. enterica strains. In conclusion, the analysis of polymorphisms in the partial rpoB sequence can discriminate S. enterica strains at the subspecies level.
authors.affiliation: a Programa de Doctorado en Ciencias Silvoagropecuarias y Veterinarias, Universidad de Chile, Santiago, Chile.
authors.forename: Marcela
authors.initials: M
authors.lastname: Fresno
authors.forename: Marlen
authors.initials: M
authors.lastname: Barreto
authors.forename: Sindy
authors.initials: S
authors.lastname: Gutierrez
authors.forename: Catherine
authors.initials: C
authors.lastname: Dougnac
authors.forename: Pedro
authors.initials: P
authors.lastname: Abalos
authors.forename: Patricio
authors.initials: P
authors.lastname: Retamal
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics*; D019779:Salmonella enterica / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016415:Sequence Alignment; D012703:Serotyping
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012324:RNA-Dependent RNA Polymerase