title: Fixed-bed biosorption of cadmium using immobilized Scenedesmus obliquus CNW-N cells on loofa (Luffa cylindrica) sponge.
abstract: A continuous fixed-bed biosorption process was established for cadmium (Cd) removal by Scenedesmus obliquus CNW-N (isolated from southern Taiwan) cells immobilized onto loofa sponge. This immobilized-cell biosorption process allows better recovery and reusability of the microalgal biomass. The growth of microalgae on the matrix support with appropriate nutrient supplementation could enhance the overall metal removal activity. Major operating parameters (e.g., feeding flow rate, cycle number of medium replacement, and particle diameter of the sponge) were studied for treatability evaluation. The most promising cell growth on the sponge support was obtained at a flow rate of 0.284 bed volume (BV)/min, sponge particle diameter of 1 cm, and with one cycle of medium replacement. The performance of fixed-bed biosorption (adsorption capacity of 38.4 mg, breakthrough time at 15.5 h) was achieved at a flow rate of 5 ml/min with an influent concentration of 7.5 mg Cd/l.
authors.affiliation: Department of Chemical and Materials Engineering, National I-Lan University, I-Lan, Taiwan.
authors.forename: Bor-Yann
authors.initials: BY
authors.lastname: Chen
authors.affiliation: Center for Biosciences and Biotechnology, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Chun-Yen
authors.initials: CY
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, PR China.
authors.forename: Wan-Qian
authors.initials: WQ
authors.lastname: Guo
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Hao-Wei
authors.initials: HW
authors.lastname: Chang
authors.affiliation: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd., Nan-Tzu, Kaohsiung City 811, Taiwan.
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Chen
authors.affiliation: Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan.
authors.forename: Duu-Jong
authors.initials: DJ
authors.lastname: Lee
authors.affiliation: Agricultural Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan; Department of Life Science, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Chieh-Chen
authors.initials: CC
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, PR China.
authors.forename: Nan-Qi
authors.initials: NQ
authors.lastname: Ren
authors.affiliation: Center for Biosciences and Biotechnology, National Cheng Kung University, Tainan, Taiwan; Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan; Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan, Taiwan. Electronic address: changjs@mail.ncku.edu.tw.
authors.forename: Jo-Shu
authors.initials: JS
authors.lastname: Chang
mesh_terms: D000327:Adsorption; D052818:Autotrophic Processes / Q000187:drug effects; D001673:Biodegradation, Environmental / Q000187:drug effects; D018533:Biomass; D002104:Cadmium / Q000302:isolation & purification*; D002244:Carbon / Q000494:pharmacology; D002245:Carbon Dioxide / Q000494:pharmacology; D018914:Cells, Immobilized / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D029776:Luffa / Q000737:chemistry*; D058086:Microalgae / Q000166:cytology / Q000187:drug effects / Q000254:growth & development; D012212:Rheology / Q000187:drug effects; D044444:Scenedesmus / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
chemical_list: D002104:Cadmium; D002245:Carbon Dioxide; D002244:Carbon
keywords: Biosorption; Cadmium; Fixed-bed process; Microalgal biosorbent; Scenedesmus obliquus