title: Gene targeting in rats using transcription activator-like effector nucleases.
abstract: The rat is a model of choice to understanding gene function and modeling human diseases. Since recent years, successful engineering technologies using gene-specific nucleases have been developed to gene edit the genome of different species, including the rat. This development has become important for the creation of new rat animals models of human diseases, analyze the role of genes and express recombinant proteins. Transcription activator-like (TALE) nucleases are designed nucleases consist of a DNA binding domain fused to a nuclease domain capable of cleaving the targeted DNA. We describe a detailed protocol for generating knockout rats via microinjection of TALE nucleases into fertilized eggs. This technology is an efficient, cost- and time-effective method for creating new rat models.
authors.affiliation: Transgenic Rats Nantes IBiSA-Centre National de Recherche Scientifique, F44093 Nantes, France; ITUN, CHU Nantes, F44000 Nantes, France; INSERM UMR 1064-Center for Research in Transplantation and Immunology, France. Electronic address: severine.menoret@univ-nantes.fr.
authors.forename: Séverine
authors.initials: S
authors.lastname: Ménoret
authors.affiliation: Transgenic Rats Nantes IBiSA-Centre National de Recherche Scientifique, F44093 Nantes, France; ITUN, CHU Nantes, F44000 Nantes, France; INSERM UMR 1064-Center for Research in Transplantation and Immunology, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Tesson
authors.affiliation: Transgenic Rats Nantes IBiSA-Centre National de Recherche Scientifique, F44093 Nantes, France; ITUN, CHU Nantes, F44000 Nantes, France; INSERM UMR 1064-Center for Research in Transplantation and Immunology, France.
authors.forename: Séverine
authors.initials: S
authors.lastname: Rémy
authors.affiliation: Transgenic Rats Nantes IBiSA-Centre National de Recherche Scientifique, F44093 Nantes, France; ITUN, CHU Nantes, F44000 Nantes, France; INSERM UMR 1064-Center for Research in Transplantation and Immunology, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Usal
authors.affiliation: Transgenic Rats Nantes IBiSA-Centre National de Recherche Scientifique, F44093 Nantes, France; ITUN, CHU Nantes, F44000 Nantes, France; INSERM UMR 1064-Center for Research in Transplantation and Immunology, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Thépenier
authors.affiliation: Transgenic Rats Nantes IBiSA-Centre National de Recherche Scientifique, F44093 Nantes, France; ITUN, CHU Nantes, F44000 Nantes, France; INSERM UMR 1064-Center for Research in Transplantation and Immunology, France.
authors.forename: Reynald
authors.initials: R
authors.lastname: Thinard
authors.affiliation: Transgenic Rats Nantes IBiSA-Centre National de Recherche Scientifique, F44093 Nantes, France; ITUN, CHU Nantes, F44000 Nantes, France; INSERM UMR 1064-Center for Research in Transplantation and Immunology, France.
authors.forename: Laure-Hélène
authors.initials: LH
authors.lastname: Ouisse
authors.affiliation: INSERM U565, CNRS UMR7196, Museum National d'Histoire Naturelle, 43 Rue Cuvier, F75005 Paris, France.
authors.forename: Anne
authors.initials: A
authors.lastname: De Cian
authors.affiliation: INSERM U565, CNRS UMR7196, Museum National d'Histoire Naturelle, 43 Rue Cuvier, F75005 Paris, France.
authors.forename: Carine
authors.initials: C
authors.lastname: Giovannangeli
authors.affiliation: INSERM U565, CNRS UMR7196, Museum National d'Histoire Naturelle, 43 Rue Cuvier, F75005 Paris, France.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Concordet
authors.affiliation: Transgenic Rats Nantes IBiSA-Centre National de Recherche Scientifique, F44093 Nantes, France; ITUN, CHU Nantes, F44000 Nantes, France; INSERM UMR 1064-Center for Research in Transplantation and Immunology, France.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Anegon
mesh_terms: D000818:Animals; D059766:DNA End-Joining Repair; D003851:Deoxyribonucleases / Q000737:chemistry / Q000235:genetics; D004624:Embryo Transfer; D004622:Embryo, Mammalian; D005260:Female; D055786:Gene Knockout Techniques; D059765:Homologous Recombination; D008845:Microinjections; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D003851:Deoxyribonucleases
keywords: Gene editing; Homologous recombination; Knockout; Non-homologous end joining; Rat model; TALE nucleases