title: Low-cost lipid production by an oleaginous yeast cultured in non-sterile conditions using model waste resources.
abstract: The yeast Metschnikowia pulcherrima, previously utilised as a biological control agent, was evaluated for its potential to produce lipids for biofuel production.
  
  
  
  Cultivation in low cost non-sterile conditions was achieved by exploiting its ability to grow at low temperature and pH and to produce natural antimicrobial compounds. Although not previously classified as oleaginous, a combination of low temperature and restricted nutrient availability triggered high levels of oil production in M. pulcherrima cultures. This regime was designed to trigger the sporulation process but prevent its completion to allow the accumulation of a subset of a normally transitional, but oil-rich, 'pulcherrima' cell type. This approach resulted in yields of up to 40% lipid, which compares favourably with other oleaginous microbes. We also demonstrate that M. pulcherrima metabolises glycerol and a diverse range of other sugars, suggesting that heterogeneous biomass could provide a suitable carbon source. M. pulcherrima also grows well in a minimal media containing no yeast extract. Finally, we demonstrate the potential of the yeast to produce lipids inexpensively on an industrial scale by culturing the yeast in a 500 L, open air, tank reactor without any significant contamination.
  
  
  
  The production of antimicrobial compounds coupled to efficient growth at low temperature and pH enables culture of this oleaginous yeast in inexpensive, non-sterile conditions providing a potential route to economic biofuel production.
authors.affiliation: Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK.|Centre for Sustainable Chemical Technologies, Department of Chemical Engineering, University of Bath, Bath BA2 7AY, UK.
authors.forename: Fabio
authors.initials: F
authors.lastname: Santamauro
authors.affiliation: Centre for Sustainable Chemical Technologies, Department of Chemical Engineering, University of Bath, Bath BA2 7AY, UK.
authors.forename: Fraeya M
authors.initials: FM
authors.lastname: Whiffin
authors.affiliation: Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK.
authors.forename: Rod J
authors.initials: RJ
authors.lastname: Scott
authors.affiliation: Centre for Sustainable Chemical Technologies, Department of Chemical Engineering, University of Bath, Bath BA2 7AY, UK.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Chuck