title: Fabrication of a heparin-PVA complex hydrogel for application as a vascular access.
abstract: A high hydrostatic pressure method, which can apply over 600 MPa pressure was employed for preparing a hydrogel of poly(vinyl alcohol) (PVA) loaded with heparin. The aim of this study was to fabricate a heparin-PVA hydrogel conduit and evaluate its potential for vascular access. Heparin-PVA complex hydrogel showed suppressed heparin release and prevented clot formation, depending on the molecular weight of the PVA. Strength of the hydrogel conduit was increased by embedding a Dacron mesh between two PVA layers. The tubular heparin-PVA complex hydrogel displayed a burst pressure of 750 mmHg. The tubular heparin-PVA complex hydrogel did not show any occlusion or burst for 2 weeks after implantation, implying that this heparin-PVA complex hydrogel shows high potential for use as a vascular access. This is the first report on the preparation of a multilayered PVA hydrogel with heparin embedded on one side only. The proposed approach could be expanded to the fabrication of various biomaterials for specific purposes.
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo, 101-0062, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Negishi
authors.forename: Kwangwoo
authors.initials: K
authors.lastname: Nam
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Kimura
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: Seiichi
authors.initials: S
authors.lastname: Funamoto
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Higami
authors.forename: Toshiya
authors.initials: T
authors.lastname: Fujisato
authors.forename: Akio
authors.initials: A
authors.lastname: Kishida
mesh_terms: D000818:Animals; D006493:Heparin / Q000737:chemistry / Q000494:pharmacology; D020100:Hydrogels / Q000737:chemistry / Q000494:pharmacology; D008422:Materials Testing; D011142:Polyvinyl Alcohol / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D013552:Swine; D062666:Vascular Access Devices
chemical_list: D020100:Hydrogels; D011142:Polyvinyl Alcohol; D006493:Heparin
keywords: heparin; high hydrostatic pressure; hydrogel; poly(vinyl alcohol); vascular access