title: Numerical investigation of two-dimensional light scattering patterns of cervical cell nuclei to map dysplastic changes at different epithelial depths.
abstract: We use an extensive set of quantitative histopathology data to construct realistic three-dimensional models of normal and dysplastic cervical cell nuclei at different epithelial depths. We then employ the finite-difference time-domain method to numerically simulate the light scattering response of these representative models as a function of the polar and azimuthal scattering angles. The results indicate that intensity and shape metrics computed from two-dimensional scattering patterns can be used to distinguish between different diagnostic categories. Our numerical study also suggests that different epithelial layers and angular ranges need to be considered separately to fully exploit the diagnostic potential of two-dimensional light scattering measurements.
authors.affiliation: Division of Cancer Research, Kemal Saracoglu Foundation for Children with Leukemia and Fight Against Cancer, Nicosia, Cyprus.
authors.forename: Dizem
authors.initials: D
authors.lastname: Arifler
authors.affiliation: Imaging Unit, Department of Integrative Oncology, British Columbia Cancer Research Center, Vancouver, BC V5Z 1L3, Canada.
authors.forename: Calum
authors.initials: C
authors.lastname: Macaulay
authors.affiliation: Department of Obstetrics and Gynecology, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center, El Paso, TX 79905, USA.
authors.forename: Michele
authors.initials: M
authors.lastname: Follen
authors.affiliation: Imaging Unit, Department of Integrative Oncology, British Columbia Cancer Research Center, Vancouver, BC V5Z 1L3, Canada.
authors.forename: Martial
authors.initials: M
authors.lastname: Guillaud
keywords: (170.0170) Medical optics and biotechnology; (170.1530) Cell analysis; (170.4580) Optical diagnostics for medicine; (170.4730) Optical pathology; (290.0290) Scattering