title: Emergence of extended-spectrum β-lactamase-producing Escherichia coli in catheter-associated urinary tract infection in neurogenic bladder patients.
abstract: BACKGROUND
  Catheter-associated urinary tract infection (CAUTI) is a common clinic problem. The purpose of this study was to investigate recent trends in CAUTI in neurogenic bladder patients focusing on extended-spectrum β-lactamase (ESBL)-producing Escherichia coli.
  
  
  METHODS
  Isolates from the urine of neurogenic bladder patients with UTI were investigated. Nine strains of ESBL-producing E coli were assayed by molecular strain typing using the Diversilab system for repetitive-sequence-based polymerase chain reaction (rep-PCR).
  
  
  RESULTS
  E coli accounted for most of the bacteria (74.1% to 81.0%) that produced ESBLs. Rep-PCR data showed that 7 out of 9 ESBL-producing E coli belonged to the same typing group with high similarity (more than 97% similarity) and that this distribution corresponded with antibiotic resistance patterns.
  
  
  CONCLUSIONS
  ESBL producing E coli strains isolated from CAUTI patients could be discriminated by rep-PCR typing using the Diversilab system in consistent with antibiotic resistance patterns.
authors.affiliation: Department of Clinical Laboratory, Hyogo Prefectural Rehabilitation Center, Kobe, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Takaba
authors.affiliation: Division of Urology, Department of Surgery Related, Kobe University Graduate School of Medicine, Kobe, Japan; Infection Control Team, Kobe University Hospital, Kobe, Japan. Electronic address: yutoshunta@hotmail.co.jp.
authors.forename: Katsumi
authors.initials: K
authors.lastname: Shigemura
authors.affiliation: Infection Control Team, Kobe University Hospital, Kobe, Japan.
authors.forename: Kayo
authors.initials: K
authors.lastname: Osawa
authors.affiliation: Department of Urology, Hyogo Prefectural Rehabilitation Hospital, Kobe, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Nomi
authors.affiliation: Division of Urology, Department of Surgery Related, Kobe University Graduate School of Medicine, Kobe, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Fujisawa
authors.affiliation: Division of Urology, Department of Surgery Related, Kobe University Graduate School of Medicine, Kobe, Japan; Infection Control Team, Kobe University Hospital, Kobe, Japan.
authors.forename: Soichi
authors.initials: S
authors.lastname: Arakawa
mesh_terms: D055499:Catheter-Related Infections / Q000175:diagnosis* / Q000382:microbiology; D004926:Escherichia coli / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000382:microbiology; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D058889:Molecular Typing; D010641:Phenotype; D001750:Urinary Bladder, Neurogenic / Q000150:complications* / Q000628:therapy*; D014552:Urinary Tract Infections / Q000175:diagnosis* / Q000382:microbiology; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D001618:beta-Lactamases
keywords: Catheter-associated urinary tract infection (CAUTI); Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli; Repetitive-sequence-based polymerase chain reaction (rep-PCR)