title: A unique mutation in a MYB gene cosegregates with the nectarine phenotype in peach.
abstract: Nectarines play a key role in peach industry; the fuzzless skin has implications for consumer acceptance. The peach/nectarine (G/g) trait was described as monogenic and previously mapped on chromosome 5. Here, the position of the G locus was delimited within a 1.1 cM interval (635 kb) based on linkage analysis of an F2 progeny from the cross 'Contender' (C, peach) x 'Ambra' (A, nectarine). Careful inspection of the genes annotated in the corresponding genomic sequence (Peach v1.0), coupled with variant discovery, led to the identification of MYB gene PpeMYB25 as a candidate for trichome formation on fruit skin. Analysis of genomic re-sequencing data from five peach/nectarine accessions pointed to the insertion of a LTR retroelement in exon 3 of the PpeMYB25 gene as the cause of the recessive glabrous phenotype. A functional marker (indelG) developed on the LTR insertion cosegregated with the trait in the CxA F2 progeny and was validated on a broad panel of genotypes, including all known putative donors of the nectarine trait. This marker was shown to efficiently discriminate between peach and nectarine plants, indicating that a unique mutational event gave rise to the nectarine trait and providing a useful diagnostic tool for early seedling selection in peach breeding programs.
authors.affiliation: Consiglio per la Ricerca e la Sperimentazione in Agricoltura - Centro di Ricerca per la Frutticoltura (CRA-FRU), Rome, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Vendramin
authors.affiliation: Parco Tecnologico Padano, Via Einstein, Loc. C.na Codazza, Lodi, Italy.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Pea
authors.affiliation: Università di Bologna, DipSA, Bologna, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Dondini
authors.affiliation: Università degli Studi di Milano, DiSAA, Milan, Italy.
authors.forename: Igor
authors.initials: I
authors.lastname: Pacheco
authors.affiliation: Consiglio per la Ricerca e la Sperimentazione in Agricoltura - Centro di Ricerca per la Frutticoltura (CRA-FRU), Rome, Italy.
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Dettori
authors.affiliation: Consiglio per la Ricerca e la Sperimentazione in Agricoltura - Centro di Ricerca per la Frutticoltura (CRA-FRU), Rome, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Gazza
authors.affiliation: IGA Technology Services, Udine, Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Scalabrin
authors.affiliation: Parco Tecnologico Padano, Via Einstein, Loc. C.na Codazza, Lodi, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Strozzi
authors.affiliation: Università di Bologna, DipSA, Bologna, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Tartarini
authors.affiliation: Università degli Studi di Milano, DiSAA, Milan, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Bassi
authors.affiliation: Consiglio per la Ricerca e la Sperimentazione in Agricoltura - Centro di Ricerca per la Frutticoltura (CRA-FRU), Rome, Italy.
authors.forename: Ignazio
authors.initials: I
authors.lastname: Verde
authors.affiliation: Parco Tecnologico Padano, Via Einstein, Loc. C.na Codazza, Lodi, Italy; Università degli Studi di Milano, DiSAA, Milan, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Rossini
mesh_terms: D001947:Breeding / Q000379:methods*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics*; D003433:Crosses, Genetic; D017931:DNA Primers / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D010641:Phenotype; D016133:Polymerase Chain Reaction; D027861:Prunus / Q000235:genetics*; D018626:Retroelements / Q000235:genetics; D064201:Trichomes / Q000235:genetics*
chemical_list: D017931:DNA Primers; D005819:Genetic Markers; D018626:Retroelements