title: [The effects of recombinant human beta-defensin-3 on expression of interleukin-17A and interleukin-22 in BEAS-2B cell].
abstract: OBJECTIVE
  To research the effects of recombinant human beta-defensin-3 (hBD-3) on expression of interleukin-17A (IL-17A) and interleukin-22 (IL-22) in BEAS-2B cell.
  
  
  METHODS
  The BEAS-2B cells were stimulated with different concentrations of hBD-3 for 6 hours and 24 hours, respectively. Toll-like receptor 2 (TLR2), IL-17A and IL-22 mRNA expression levels were determined by real-time PCR, and the expression levels of IL-17A and IL-22 protein were examined by enzyme linked immune-sorbent assay.
  
  
  RESULTS
  TLR2 mRNA in BEAS-2B cells were significantly increased in a concentration-and time-dependent manner after stimulating by hBD-3 for 24 hours compared to 6 hours. The IL-17A has significantly increased in mRNA and protein levels stimulated 24 hours in a concentration of 100 ng/ml, however, IL-17A mRNA expression has increased while protein didn't change stimulated 6 hours in a concentration of 50 ng/ml. The IL-22 mRNA and protein expression reached peak levels after stimulating in a concentration of 50 ng/ml of hBD-3 while IL-22 expression declined in mRNA and protein levels as the concentration of hBD-3 increased.
  
  
  CONCLUSIONS
  Recombinant hBD-3 can up-regulated the expression of TLR2, IL-17A and IL-22, lower concentration of hBD-3 mainly increased the expression of IL-22 while higher concentration of hBD-3 mainly increased the expression of IL-17A. These results show that different concentrations of hBD-3 maybe activate different transcription factors which was mediated by TLR2, initiating host immune response.
authors.affiliation: Department of Otolaryngology & Head and Neck Surgery, First Hospital of Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Bing-Ya
authors.initials: BY
authors.lastname: Guo
authors.affiliation: Department of Otolaryngology & Head and Neck Surgery, First Hospital of Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Guang-Cheng
authors.initials: GC
authors.lastname: Xie
authors.affiliation: Department of Otolaryngology & Head and Neck Surgery, First Hospital of Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Zhao-Jun
authors.initials: ZJ
authors.lastname: Duan
authors.affiliation: Department of Otolaryngology & Head and Neck Surgery, First Hospital of Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Xia
mesh_terms: D002460:Cell Line; D006801:Humans; D020381:Interleukin-17 / Q000235:genetics* / Q000378:metabolism; D007378:Interleukins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000378:metabolism; D023083:beta-Defensins / Q000235:genetics / Q000378:metabolism*; D000097043:Interleukin-22
chemical_list: D020381:Interleukin-17; D007378:Interleukins; D012333:RNA, Messenger; D051195:Toll-Like Receptor 2; D023083:beta-Defensins; D000097043:Interleukin-22; C422950:DEFB103A protein, human