title: Assaying mRNA deadenylation in vivo.
abstract: Deadenylation is the removal of poly(A) tails from mRNA. Here, we present two methods for assaying deadenylation in vivo. The first is a method for measuring bulk poly(A) tail lengths. When combined with a block in transcription, the method can be used for measuring the rate of bulk poly(A) tail shortening. The second is an RT-PCR method to determine the poly(A) tail lengths of individual RNAs. Again in combination with a block of transcription, the method permits the rate of deadenylation of an individual RNA to be measured.
authors.affiliation: Institute of Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, Kurt-Mothes-Strasse 3, 06120, Halle, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Temme
authors.forename: Elmar
authors.initials: E
authors.lastname: Wahle
mesh_terms: D000818:Animals; D004330:Drosophila; D011061:Poly A / Q000737:chemistry*; D026723:Polyadenylation / Q000502:physiology; D012333:RNA, Messenger / Q000737:chemistry*
chemical_list: D012333:RNA, Messenger; D011061:Poly A