title: Versican expression in myoepithelial cells from carcinomas in canine mixed mammary tumors.
abstract: The matrix of canine mixed mammary tumors (CMMTs) consists of proliferating spindle cells of possible myoepithelial origin, as well as myxomatous tissue, cartilage matrix and/or bone. Among the multiple components of this tumor extracellular matrix, versican probably plays a prominent role due to its importance in tumor progression, cell proliferation and differentiation. However, there are few data related to a possible association between versican expression and the state of myoepithelial cell differentiation in CMMTs. Using immunohistochemistry and histochemistry, the objective of this study was to evaluate the expression of versican, sulfated proteoglycans and mucopolysaccharides in myoepithelial cells at different stages of differentiation and to explore a potential relationship with p63 and α-smooth muscle actin (SMA) expression. A significant difference in versican expression was observed among the different stages of myoepithelial cell differentiation with an inverse correlation between versican and p63/SMA expression. These results suggest that at an early stage of proliferation, myoepithelial cells acquire a phenotype consistent with a role in chondrogenesis. Moreover, myoepithelial cells showed an affinity for safranin and periodic acid-Schiff staining at different stages of proliferation supporting the myoepithelial origin of spindle cells from CMMTs.
authors.affiliation: Department of General Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Karine A
authors.initials: KA
authors.lastname: Damasceno
authors.affiliation: Fepagro Animal Health - Desidério Finamor Institute of Veterinary Research (IPVDF), Eldorado do Sul, RS, Brazil.
authors.forename: Angélica C
authors.initials: AC
authors.lastname: Bertagnolli
authors.affiliation: Department of Pathology and Clinics, School of Veterinary Medicine and Zootechny, Universidade Federal da Bahia, Salvador, BA, Brazil.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Estrela-Lima
authors.affiliation: Department of General Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Bruna S
authors.initials: BS
authors.lastname: Rabelo
authors.affiliation: Department of General Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Liliane C
authors.initials: LC
authors.lastname: Campos
authors.affiliation: Department of Pathology and Clinics, School of Veterinary Medicine and Zootechny, Universidade Federal da Bahia, Salvador, BA, Brazil.
authors.forename: Lorena G R
authors.initials: LG
authors.lastname: Ribeiro
authors.affiliation: Department of General Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. Electronic address: cassalig@icb.ufmg.br.
authors.forename: Geovanni D
authors.initials: GD
authors.lastname: Cassali
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D011508:Chondroitin Sulfate Proteoglycans / Q000235:genetics* / Q000378:metabolism; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D004847:Epithelial Cells; D005260:Female; D015870:Gene Expression; D006025:Glycosaminoglycans / Q000235:genetics* / Q000378:metabolism; D007150:Immunohistochemistry; D015674:Mammary Neoplasms, Animal / Q000378:metabolism / Q000473:pathology*; D009208:Myoepithelioma / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism; D053675:Versicans / Q000235:genetics* / Q000378:metabolism
chemical_list: D000199:Actins; D011508:Chondroitin Sulfate Proteoglycans; D006025:Glycosaminoglycans; D010750:Phosphoproteins; D053675:Versicans
keywords: Canine; Chondrogenesis; Mixed mammary tumor; Myoepithelial cell; Versican