title: Evolution of bone biomechanical properties at the micrometer scale around titanium implant as a function of healing time.
abstract: The characterization of the biomechanical properties of newly formed bone tissue around implants is important to understand the osseointegration process. The objective of this study is to investigate the evolution of elastic properties of newly formed bone tissue as a function of healing time. To do so, nanoindentation and micro-Brillouin scattering techniques are coupled following a multimodality approach using histological analysis. Coin-shaped implants were placed in vivo at a distance of 200 µm from the cortical bone surface, leading to an initially empty cavity. Two rabbits were sacrificed after 7 and 13 weeks of healing time. The histological analyses allow us to distinguish mature and newly formed bone tissue. The bone mechanical properties were measured in mature and newly formed bone tissue. Analysis of variance and Tukey-Kramer tests reveals a significant effect of healing time on the indentation modulus and ultrasonic velocities of bone tissue. The results show that bone mass density increases by 12.2% (2.2% respectively) between newly formed bone at 7 weeks (13 weeks respectively) and mature bone. The dependence of bone properties on healing time may be explained by the evolution of bone microstructure and mineralization.
authors.affiliation: CNRS, Laboratoire Modélisation et Simulation Multi Echelle, MSME UMR CNRS 8208, 61, avenue du Général de Gaulle, 94010 Créteil, Cedex, France.
authors.forename: Romain
authors.initials: R
authors.lastname: Vayron
authors.forename: Mami
authors.initials: M
authors.lastname: Matsukawa
authors.forename: Ryo
authors.initials: R
authors.lastname: Tsubota
authors.forename: Vincent
authors.initials: V
authors.lastname: Mathieu
authors.forename: Etienne
authors.initials: E
authors.lastname: Barthel
authors.forename: Guillaume
authors.initials: G
authors.lastname: Haiat
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D015519:Bone Density; D055119:Elastic Modulus; D005260:Female; D008422:Materials Testing; D016348:Osseointegration; D019736:Prostheses and Implants; D011817:Rabbits; D013499:Surface Properties; D013977:Tibia / Q000502:physiology*; D013997:Time Factors; D014025:Titanium; D014945:Wound Healing
chemical_list: D014025:Titanium