title: Arabidopsis protein phosphatase DBP1 nucleates a protein network with a role in regulating plant defense.
abstract: Arabidopsis thaliana DBP1 belongs to the plant-specific family of DNA-binding protein phosphatases. Although recently identified as a novel host factor mediating susceptibility to potyvirus, little is known about DBP1 targets and partners and the molecular mechanisms underlying its function. Analyzing changes in the phosphoproteome of a loss-of-function dbp1 mutant enabled the identification of 14-3-3λ isoform (GRF6), a previously reported DBP1 interactor, and MAP kinase (MAPK) MPK11 as components of a small protein network nucleated by DBP1, in which GRF6 stability is modulated by MPK11 through phosphorylation, while DBP1 in turn negatively regulates MPK11 activity. Interestingly, grf6 and mpk11 loss-of-function mutants showed altered response to infection by the potyvirus Plum pox virus (PPV), and the described molecular mechanism controlling GRF6 stability was recapitulated upon PPV infection. These results not only contribute to a better knowledge of the biology of DBP factors, but also of MAPK signalling in plants, with the identification of GRF6 as a likely MPK11 substrate and of DBP1 as a protein phosphatase regulating MPK11 activity, and unveils the implication of this protein module in the response to PPV infection in Arabidopsis.
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-C.S.I.C, Ciudad Politécnica de la Innovación, Valencia, Spain.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Carrasco
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-C.S.I.C, Ciudad Politécnica de la Innovación, Valencia, Spain.
authors.forename: María José
authors.initials: MJ
authors.lastname: Castelló
authors.affiliation: Department of Stress and Developmental Biology, Leibniz Institute of Plant Biochemistry, Halle, Germany.
authors.forename: Kai
authors.initials: K
authors.lastname: Naumann
authors.affiliation: Department of Stress and Developmental Biology, Leibniz Institute of Plant Biochemistry, Halle, Germany.
authors.forename: Ines
authors.initials: I
authors.lastname: Lassowskat
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-C.S.I.C, Ciudad Politécnica de la Innovación, Valencia, Spain.
authors.forename: Marisa
authors.initials: M
authors.lastname: Navarrete-Gómez
authors.affiliation: Department of Stress and Developmental Biology, Leibniz Institute of Plant Biochemistry, Halle, Germany.
authors.forename: Dierk
authors.initials: D
authors.lastname: Scheel
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-C.S.I.C, Ciudad Politécnica de la Innovación, Valencia, Spain; Department of Stress and Developmental Biology, Leibniz Institute of Plant Biochemistry, Halle, Germany.
authors.forename: Pablo
authors.initials: P
authors.lastname: Vera
mesh_terms: D048948:14-3-3 Proteins / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D029681:Arabidopsis Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D054884:Host-Pathogen Interactions; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D009154:Mutation; D010749:Phosphoprotein Phosphatases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D010935:Plant Diseases / Q000235:genetics / Q000821:virology; D017800:Potyvirus / Q000502:physiology*; D060066:Protein Interaction Maps
chemical_list: D048948:14-3-3 Proteins; C117576:AFT1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; D010750:Phosphoproteins; C557478:MPK11 protein, Arabidopsis; D020928:Mitogen-Activated Protein Kinases; C000592774:DBP1 protein, Arabidopsis; D010749:Phosphoprotein Phosphatases