title: Coinfection of Fusobacterium nucleatum and Actinomyces israelii in mastoiditis diagnosed by next-generation DNA sequencing.
abstract: Some bacterial infections involve potentially complex mixtures of species that can now be distinguished using next-generation DNA sequencing. We present a case of mastoiditis where Gram stain, culture, and molecular diagnosis were nondiagnostic or discrepant. Next-generation sequencing implicated coinfection of Fusobacterium nucleatum and Actinomyces israelii, resolving these diagnostic discrepancies.
authors.affiliation: Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Salipante
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Hoogestraat
authors.forename: April N
authors.initials: AN
authors.lastname: Abbott
authors.forename: Dhruba J
authors.initials: DJ
authors.lastname: SenGupta
authors.forename: Lisa A
authors.initials: LA
authors.lastname: Cummings
authors.forename: Susan M
authors.initials: SM
authors.lastname: Butler-Wu
authors.forename: Karen
authors.initials: K
authors.lastname: Stephens
authors.forename: Brad T
authors.initials: BT
authors.lastname: Cookson
authors.forename: Noah G
authors.initials: NG
authors.lastname: Hoffman
mesh_terms: D000190:Actinomyces / Q000302:isolation & purification*; D000196:Actinomycosis / Q000175:diagnosis / Q000382:microbiology; D060085:Coinfection / Q000175:diagnosis* / Q000382:microbiology*; D005674:Fusobacterium Infections / Q000175:diagnosis / Q000382:microbiology; D016967:Fusobacterium nucleatum / Q000235:genetics / Q000302:isolation & purification*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006801:Humans; D008297:Male; D008417:Mastoiditis / Q000175:diagnosis* / Q000382:microbiology*; D008875:Middle Aged