title: Antioxidant property of aerial parts and root of Phyllanthus fraternus Webster, an important medicinal plant.
abstract: In present study free radical scavenging potential of aerial parts and root of Phyllanthus fraternus was investigated. Extraction was done in water and ethanol. Total antioxidant capacity was measured by DPPH free radical scavenging method; ethanolic extract of aerial part was most potent in activity with 50% inhibition at 258 μg/mL concentration. Lipid peroxidation (LPO) was measured in terms of thiobarbituric acid-reactive substances (TBARS) by using egg-yolk homogenates as lipid-rich media with EC₅₀ of aerial part (ethanolic) 1522 μg/mL which was found to be most active. Superoxide (SO) radical scavenging activity was measured using riboflavin-light-nitroblue tetrazolium assay. Ethanolic and aqueous extract of both aerial part and root was almost similar in superoxide radical scavenging activity. Reducing power was determined on the basis of Fe³⁺-Fe⁺ transformation in the presence of extract. Total phenolic and flavonoid contents were also measured by spectroscopic method. Results showed that the ethanolic fraction of aerial part is most active towards antioxidant potential and this activity is related to its polyphenolic content and reducing potential. Thus, P. fraternus extract can be used as potent natural antioxidant.
authors.affiliation: Department of Botany, MMV, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, India.
authors.forename: Richa
authors.initials: R
authors.lastname: Upadhyay
authors.affiliation: Department of Applied Science, Kashi Institute of Technology, Varanasi, Uttar Pradesh 221307, India.
authors.forename: Jitendra Kumar
authors.initials: JK
authors.lastname: Chaurasia
authors.affiliation: Department of Botany, MMV, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, India.
authors.forename: Kavindra Nath
authors.initials: KN
authors.lastname: Tiwari
authors.affiliation: Department of Zoology, MMV, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, India.
authors.forename: Karuna
authors.initials: K
authors.lastname: Singh
mesh_terms: D000704:Analysis of Variance; D000975:Antioxidants / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D016166:Free Radical Scavengers / Q000378:metabolism*; D007194:India; D015227:Lipid Peroxidation; D010084:Oxidation-Reduction; D029803:Phyllanthus / Q000737:chemistry*; D035261:Plant Components, Aerial / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D018517:Plant Roots / Q000737:chemistry*; D013053:Spectrophotometry
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts