title: Properties of biologic scaffolds and their response to mesenchymal stem cells.
abstract: OBJECTIVE
  The purpose of this study was to examine, in vitro, the cellular response of human mesenchymal stem cells (MSCs) to sample types of commercially available scaffolds in comparison with control, native tendon tissue (fresh-frozen rotator cuff tendon allograft).
  
  
  METHODS
  MSCs were defined by (1) colony-forming potential; (2) ability to differentiate into tendon, cartilage, bone, and fat tissue; and (3) fluorescence-activated cell sorting analysis (CD73, CD90, CD45). Samples were taken from fresh-frozen human rotator cuff tendon (allograft), human highly cross-linked collagen membrane (Arthroflex; LifeNet Health, Virginia Beach, VA), porcine non-cross-linked collagen membrane (Mucograft; Geistlich Pharma, Lucerne, Switzerland), a human platelet-rich fibrin matrix (PRF-M), and a fibrin matrix based on platelet-rich plasma (ViscoGel; Arthrex, Naples, FL). Cells were counted for adhesion (24 hours), thymidine assay for cell proliferation (96 hours), and live/dead stain for viability (168 hours). Histologic analysis was performed after 21 days, and the unloaded scaffolds were scanned with electron microscopy.
  
  
  RESULTS
  MSCs were successfully differentiated into all cell lines. A significantly greater number of cells adhered to both the non-cross-linked porcine collagen scaffold and PRF-M. Cell activity (proliferation) was significantly higher in the non-cross-linked porcine collagen scaffold compared with PRF-M and fibrin matrix based on platelet-rich plasma. There were no significant differences found in the results of the live/dead assay.
  
  
  CONCLUSIONS
  Significant differences in the response of human MSCs to biologic scaffolds existed. MSC adhesion, proliferation, and scaffold morphology evaluated by histologic analysis and electron microscopy varied throughout the evaluated types of scaffolds. Non-cross-linked porcine collagen scaffolds showed superior results for cell adhesion and proliferation, as well as on histologic evaluation.
  
  
  CONCLUSIONS
  This study enables the clinician and scientist to choose scaffold materials according to their specific interaction with MSCs.
authors.affiliation: Department of Trauma and Orthopaedic Surgery, Trauma Center, Murnau, Germany.
authors.forename: Knut
authors.initials: K
authors.lastname: Beitzel
authors.affiliation: Department of Orthopaedic Surgery, University of Connecticut Health Center, Farmington, Connecticut, U.S.A.
authors.forename: Mary Beth
authors.initials: MB
authors.lastname: McCarthy
authors.affiliation: Department of Orthopaedic Surgery, University of Connecticut Health Center, Farmington, Connecticut, U.S.A.
authors.forename: Mark P
authors.initials: MP
authors.lastname: Cote
authors.affiliation: Department of Orthopaedic Surgery, University of Connecticut Health Center, Farmington, Connecticut, U.S.A.
authors.forename: Ryan P
authors.initials: RP
authors.lastname: Russell
authors.affiliation: Department of Orthopaedic Surgery, University of Connecticut Health Center, Farmington, Connecticut, U.S.A.
authors.forename: John
authors.initials: J
authors.lastname: Apostolakos
authors.affiliation: Department of Orthopaedic Surgery, University of Connecticut Health Center, Farmington, Connecticut, U.S.A.
authors.forename: Daisy M
authors.initials: DM
authors.lastname: Ramos
authors.affiliation: Department of Orthopaedic Surgery, University of Connecticut Health Center, Farmington, Connecticut, U.S.A.
authors.forename: Sangamesh G
authors.initials: SG
authors.lastname: Kumbar
authors.affiliation: Department of Orthopaedic Sports Medicine, Technical University of Munich, Munich, Germany.
authors.forename: Andreas B
authors.initials: AB
authors.lastname: Imhoff
authors.affiliation: Department of Orthopaedic Surgery, University of Connecticut Health Center, Farmington, Connecticut, U.S.A.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Arciero
authors.affiliation: Department of Orthopaedic Surgery, University of Connecticut Health Center, Farmington, Connecticut, U.S.A.. Electronic address: mazzocca@uchc.edu.
authors.forename: Augustus D
authors.initials: AD
authors.lastname: Mazzocca
mesh_terms: D000818:Animals; D002356:Cartilage / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D002469:Cell Separation; D003094:Collagen; D003114:Colony-Forming Units Assay; D005434:Flow Cytometry; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D053657:Platelet-Rich Plasma; D017006:Rotator Cuff / Q000502:physiology / Q000637:transplantation; D013552:Swine; D013710:Tendons / Q000502:physiology; D054457:Tissue Scaffolds
chemical_list: D003094:Collagen