title: Immobilization of bacterial S-layer proteins from Caulobacter crescentus on iron oxide-based nanocomposite: synthesis and spectroscopic characterization of zincite-coated Fe₂O₃ nanoparticles.
abstract: Zinc oxide was coated on Fe2O3 nanoparticles using sol-gel spin-coating. Caulobacter crescentus have a crystalline surface layer (S-layer), which consist of one protein or glycoprotein species. The immobilization of bacterial S-layers obtained from C. crescentus on zincite-coated nanoparticles of iron oxide was investigated. The SDS PAGE results of S-layers isolated from C. crescentus showed the weight of 50 KDa. Nanoparticles of the Fe2O3 and zinc oxide were synthesized by a sol-gel technique. Fe2O3 nanoparticles with an average size of 50 nm were successfully prepared by the proper deposition of zinc oxide onto iron oxide nanoparticles surface annealed at 450 °C. The samples were characterized by field-emission scanning electron microscope (FESEM), atomic force microscopy (AFM), powder X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FT-IR).
authors.affiliation: Nanotechnology and Advanced Materials Institute, Isfahan University of Technology, Isfahan 84156-83111, Islamic Republic of Iran. Electronic address: ned.habibi@gmail.com.
authors.forename: Neda
authors.initials: N
authors.lastname: Habibi
mesh_terms: D016935:Caulobacter crescentus / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005290:Ferric Compounds / Q000737:chemistry*; D055767:Immobilized Proteins / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D013052:Spectrometry, X-Ray Emission; D014961:X-Ray Diffraction; D017967:Zinc Compounds / Q000737:chemistry*; D015034:Zinc Oxide / Q000737:chemistry
chemical_list: D005290:Ferric Compounds; D055767:Immobilized Proteins; D008562:Membrane Glycoproteins; C055764:S-layer proteins; D017967:Zinc Compounds; C000499:ferric oxide; D015034:Zinc Oxide
keywords: Bacterial S-layer; Caulobacter crescentus; Immobilization; Maghemite-based nanocomposite