title: Stro-1-positive BMSCs predict postoperative periprosthetic bone mineral density outcomes in uncemented total hip arthroplasty patients.
abstract: BACKGROUND
  Bone marrow cell profiles are variable after total hip arthroplasty (THA), including variable levels of Stro-1+ and bone morphogenetic protein receptor (BMPRs)+ cells. We investigated the impact of bone marrow cell profiles on changes in periprosthetic bone mineral density (BMD) in uncemented THA patients.
  
  
  METHODS
  Bone marrow aspirates were collected from the metaphyseal region of discarded femoral heads from 24 consecutive THA patients (12 men and 12 women; mean age 66.7 ± 11.0 years; range 52-87 years) treated from March 2009 to March 2011 at a single facility. Perioperative proportions of Stro-1+ and BMPR+ cells in femoral heads were assessed by flow cytometry. Follow-up examined the proximal femur Gruen zones R1 and R7 at 1 week and at 3, 6, and 12 months after THA, using dual-energy X-ray absorptiometry. Associations between BMD loss and age, gender, BMPRs+, and Stro-1+ were analyzed.
  
  
  RESULTS
  At 3 months, R1 and R7 BMD decreased by 4.4% and 6.4%, respectively (P<0.05). At 12 months, the overall BMD decreases in R1 and R7 were 10.2% and 1%, respectively (P<0.05). Higher Stro-1+ cells proportion predicted R7 BMD increases at all time points (P<0.05) and R1 BMD increases at 6 and 12 months (P<0.05). BMPR1a+ proportion was associated with BMD increases at 6 months in the R1 region. BMPR2+ was not significantly associated with BMD (P>0.05).
  
  
  CONCLUSIONS
  Elevated Stro-1+ bone marrow cell profile may be a useful prognostic indicator for uncemented THA patients.
authors.affiliation: Department of Orthopaedic, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China (mainland).
authors.forename: Yi
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Orthopaedic, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China (mainland).
authors.forename: Xiaomiao
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Orthopaedic, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China (mainland).
authors.forename: Yurun
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Biomedical Engineering and Orthopedic Surgery, Wayne State University, Detroit, USA.
authors.forename: Weiping
authors.initials: W
authors.lastname: Ren
authors.affiliation: Department of Orthopaedic, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China (mainland).
authors.forename: Weili
authors.initials: W
authors.lastname: Wang
mesh_terms: D015502:Absorptiometry, Photon; D000368:Aged; D000369:Aged, 80 and over; D000954:Antigens, Surface / Q000378:metabolism*; D019644:Arthroplasty, Replacement, Hip; D001843:Bone Cements / Q000627:therapeutic use*; D015519:Bone Density; D001854:Bone Marrow Cells / Q000378:metabolism* / Q000473:pathology; D052005:Bone Morphogenetic Protein Receptors, Type I / Q000378:metabolism; D052006:Bone Morphogenetic Protein Receptors, Type II / Q000378:metabolism; D005260:Female; D005269:Femur / Q000000981:diagnostic imaging / Q000473:pathology; D006622:Hip Prosthesis; D006801:Humans; D008297:Male; D008875:Middle Aged; D011182:Postoperative Care; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology; D016896:Treatment Outcome
chemical_list: D000954:Antigens, Surface; D001843:Bone Cements; C525591:STRO-1 antigen, human; C498211:BMPR1A protein, human; C498218:BMPR2 protein, human; D052005:Bone Morphogenetic Protein Receptors, Type I; D052006:Bone Morphogenetic Protein Receptors, Type II