title: An evaluation of serotyping of Avibacterium paragallinarum by use of a multiplex polymerase chain reaction.
abstract: In the present study, the ability of a recently proposed multiplex polymerase chain reaction (mPCR) to determine the serogroups (A, B, and C) of Avibacterium paragallinarum was evaluated. A total of 12 reference strains and 69 field isolates of Av. paragallinarum from Ecuador, Mexico, Panama, and Peru were included in the study. With some exceptions (which were serotyped in the current study), all of the isolates and strains had been previously examined by 2 serotyping schemes (Page and Kume) or were the formal reference strains for the schemes. Three of 6 (50%) reference strains of serogroup A, 2 (100%) of serogroup B, and 1 of 4 (25%) reference strains of serogroup C were correctly serotyped by the mPCR. With the field isolates, the mPCR correctly recognized 16 of the 17 serogroup A isolates, 10 of the 12 serogroup B isolates, and 18 of the 37 serogroup C isolates. Overall, the specificity and sensitivity of the PCR test was as follows: 82.6% and 87.3% (serogroup A), 85.7% and 71.9% (serogroup B), and 46.3% and 100% (serogroup C). The poor performance of the mPCR in terms of recognition of serogroup C isolates (low sensitivity of 46.3%) and the relatively high level of uncertainty about the accuracy of the serogroup A and B results (specificity of 87.3% and 71.9%, respectively) means that the assay cannot be recommended as a replacement for conventional serotyping.
authors.affiliation: 1Edgardo Soriano-Vargas, CIESA-FMVZ-UAEM, Instituto Literario No. 100, Col. Centro, Toluca 50000, México. soriano@uaemex.mx.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Morales-Erasto
authors.forename: José de Jesús
authors.initials: Jde J
authors.lastname: Posadas-Quintana
authors.forename: Manolo
authors.initials: M
authors.lastname: Fernández-Díaz
authors.forename: Luis E
authors.initials: LE
authors.lastname: Saravia
authors.forename: José Simón
authors.initials: JS
authors.lastname: Martínez-Castañeda
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Blackall
authors.forename: Edgardo
authors.initials: E
authors.lastname: Soriano-Vargas
mesh_terms: D000818:Animals; D002645:Chickens; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D015661:Pasteurellaceae / Q000145:classification*; D016871:Pasteurellaceae Infections / Q000382:microbiology / Q000662:veterinary*; D011201:Poultry Diseases / Q000175:diagnosis / Q000382:microbiology; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000379:methods / Q000662:veterinary*
keywords: Avibacterium paragallinarum; infectious coryza; polymerase chain reaction; poultry; serotyping