title: Green coffee polyphenols do not attenuate features of the metabolic syndrome and improve endothelial function in mice fed a high fat diet.
abstract: We have investigated the effects of the major polyphenol in coffee, chlorogenic acid (CGA), on obesity, glucose intolerance, insulin resistance, systemic oxidative stress and endothelial dysfunction in a mouse model of the metabolic syndrome. Thirty C57BL6 mice were randomly divided into (n=10/group) (i) normal diet (ND), (ii) high fat diet (HFD), or (iii) high fat diet supplemented with 0.5% w/w green coffee bean extract (GCE) rich in chlorogenic acid (HFD+GCE). The high fat diet consisted of 28% fat and all animals were maintained on their diets for 12 weeks. The mice fed a HFD and HFD+GCE displayed symptoms of the metabolic syndrome compared to their normal fed counterparts, although no endothelial dysfunction was detected in the abdominal aortas after 12 weeks. GCE did not attenuate HFD-induced obesity, glucose intolerance, insulin resistance or systemic oxidative stress. Furthermore, GCE did not protect against ex vivo oxidant (hypochlorous acid)-induced endothelial dysfunction.
authors.affiliation: School of Medicine & Pharmacology, University of Western Australia, Perth, Australia.
authors.forename: J D
authors.initials: JD
authors.lastname: Li Kwok Cheong
authors.affiliation: School of Medicine & Pharmacology, University of Western Australia, Perth, Australia.
authors.forename: K D
authors.initials: KD
authors.lastname: Croft
authors.affiliation: School of Medicine & Pharmacology, University of Western Australia, Perth, Australia.
authors.forename: P D
authors.initials: PD
authors.lastname: Henry
authors.affiliation: Laboratory for Metabolic Dysfunction, UWA Centre for Medical Research, Harry Perkins Institute of Medical Research, Perth, Australia.
authors.forename: V
authors.initials: V
authors.lastname: Matthews
authors.affiliation: School of Medicine & Pharmacology, University of Western Australia, Perth, Australia.
authors.forename: J M
authors.initials: JM
authors.lastname: Hodgson
authors.affiliation: School of Medicine & Pharmacology, University of Western Australia, Perth, Australia. Electronic address: natalie.ward@uwa.edu.au.
authors.forename: N C
authors.initials: NC
authors.lastname: Ward
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal / Q000187:drug effects / Q000503:physiopathology; D001835:Body Weight / Q000187:drug effects; D003069:Coffee / Q000737:chemistry*; D059305:Diet, High-Fat / Q000009:adverse effects*; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D005951:Glucose Tolerance Test; D007333:Insulin Resistance; D008297:Male; D024821:Metabolic Syndrome / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000187:drug effects; D059808:Polyphenols / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D014664:Vasodilation / Q000187:drug effects
chemical_list: D003069:Coffee; D059808:Polyphenols
keywords: Atherosclerosis; Chlorogenic acid; Coffee; Endothelial dysfunction; Metabolic syndrome