title: High-resolution melting analysis for accurate detection of BRAF mutations: a systematic review and meta-analysis.
abstract: The high-resolution melting curve analysis (HRMA) might be a good alternative method for rapid detection of BRAF mutations. However, the accuracy of HRMA in detection of BRAF mutations has not been systematically evaluated. We performed a systematic review and meta-analysis involving 1324 samples from 14 separate studies. The overall sensitivity of HRMA was 0.99 (95% confidence interval (CI) = 0.75-0.82), and the overall specificity was very high at 0.99 (95% CI = 0.94-0.98). The values for the pooled positive likelihood ratio, negative likelihood ratio, and diagnostic odds ratio were 68.01 (95% CI = 25.33-182.64), 0.06 (95% CI = 0.03-0.11), and 1263.76 (95% CI = 393.91-4064.39), respectively. The summary receiver operating characteristic curve for the same data shows an area of 1.00 and a Q* value of 0.97. The high sensitivity and specificity, simplicity, low cost, less labor or time and rapid turnaround make HRMA a good alternative method for rapid detection of BRAF mutations in the clinical practice.
authors.affiliation: Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing, PR China.
authors.forename: Dong
authors.initials: D
authors.lastname: Chen
authors.affiliation: Research and service centre of laboratory medicine, Chengdu Military general Hospital, Chengdu, PR China.
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Wang
authors.affiliation: Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing, PR China.
authors.forename: Zheng-Ran
authors.initials: ZR
authors.lastname: Chuai
authors.affiliation: Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing, PR China.
authors.forename: Jun-Fu
authors.initials: JF
authors.lastname: Huang
authors.affiliation: Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing, PR China.
authors.forename: Yun-Xia
authors.initials: YX
authors.lastname: Wang
authors.affiliation: Research Center for Nutrition and Food Safety, Institute of Military Preventive Medicine, Third Military Medical University, Chongqing, PR China.
authors.forename: Kai
authors.initials: K
authors.lastname: Liu
authors.affiliation: Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing, PR China.
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Zhang
authors.affiliation: Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing, PR China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing, PR China.
authors.forename: Da-Chuan
authors.initials: DC
authors.lastname: Shi
authors.affiliation: Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing, PR China.
authors.forename: Wei-Ling
authors.initials: WL
authors.lastname: Fu
mesh_terms: D006801:Humans; D009154:Mutation; D011110:Polymorphism, Genetic; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics*; D012372:ROC Curve; D044366:Transition Temperature
chemical_list: C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf