title: Comparative study on effects of single and multiple oral administration of mungbean (Phaseolus radiatus L.) seed extract on the pharmacokinetics of aconitine by UHPLC-MS.
abstract: The study was aimed to investigate the effects of single and multiple oral administration of mungbean (Phaseolus radiatus L.) seed extract (ME) on the pharmacokinetics of aconitine in rats. The Sprague-Dawley rats were randomly divided into three groups (six rats each group). In group 1, rats were orally administered 500 µg/kg aconitine after receiving a single oral dose of 1 g/kg ME. In group 2, rats were orally administered with 500 µg/kg aconitine at day 7 of treatment with 1 g/kg/day ME. In group 3, rats were orally administered with 500 µg/kg aconitine. Blood samples were collected at different time points (0.083, 0.25, 0.5, 1.0, 1.5, 2.0, 4.0, 6.0, 8.0 and 10.0 h). The concentration of aconitine in rats plasma was determined by a fully validated ultra-high-performance liquid chromatography coupled with mass spectrometry method. The results showed that single and multiple oral co-administration of ME significantly altered the pharmacokinetic parameters of aconitine.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
authors.forename: Enze
authors.initials: E
authors.lastname: Gao
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Yu
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.forename: Hualing
authors.initials: H
authors.lastname: Li
authors.forename: Pei
authors.initials: P
authors.lastname: Wang
authors.forename: Yingqing
authors.initials: Y
authors.lastname: Wei
authors.forename: Yunli
authors.initials: Y
authors.lastname: Zhao
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Yu
mesh_terms: D000157:Aconitine / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D016014:Linear Models; D027805:Phaseolus / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D010936:Plant Extracts; D000157:Aconitine
keywords: Phaseolus radiatus L; UHPLC-MS; aconitine; pharmacokinetics; quantitative analysis