title: Membrane-bound delta-like 1 homolog (Dlk1) promotes while soluble Dlk1 inhibits myogenesis in C2C12 cells.
abstract: Delta-like 1 homolog (Dlk1) is important in myogenesis. However, the roles of different Dlk1 isoforms have not been investigated. In C2C12 cell lines producing different Dlk1 isoforms, membrane-bound Dlk1 promoted the hypertrophic phenotype and a higher fusion rate, whereas soluble Dlk1 inhibited myotube formation. Inversed expression patterns of genes related to myogenic differentiation further support these phenotypic changes. In addition, temporal expression and balance between the Dlk1 isoforms have a regulatory role in myogenesis in vivo. Collectively, Dlk1 isoforms have distinctive effects on myogenesis, and its regulation during myogenesis is critical for normal muscle development.
authors.affiliation: Department of Animal Sciences, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Sangsu
authors.initials: S
authors.lastname: Shin
authors.affiliation: Department of Animal Sciences, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Yeunsu
authors.initials: Y
authors.lastname: Suh
authors.affiliation: Department of Animal Sciences, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Henry N
authors.initials: HN
authors.lastname: Zerby
authors.affiliation: Department of Animal Sciences, The Ohio State University, Columbus, OH 43210, USA. Electronic address: lee.2626@osu.edu.
authors.forename: Kichoon
authors.initials: K
authors.lastname: Lee
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins; D002454:Cell Differentiation; D002460:Cell Line; D048429:Cell Size; D015870:Gene Expression; D005786:Gene Expression Regulation; D036341:Intercellular Signaling Peptides and Proteins / Q000502:physiology*; D008565:Membrane Proteins / Q000502:physiology; D051379:Mice; D008813:Mice, Inbred ICR; D024510:Muscle Development; D018485:Muscle Fibers, Skeletal / Q000502:physiology; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism; D020033:Protein Isoforms / Q000502:physiology; D012756:Sheep; D012995:Solubility
chemical_list: D002135:Calcium-Binding Proteins; C512925:Dlk1 protein, mouse; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009124:Muscle Proteins; D020033:Protein Isoforms
keywords: C2C12 cell; Membrane-bound Dlk1; Muscle development; Soluble Dlk1; myogenesis