title: Effects of telmisartan on lipid metabolisms and proinflammatory factors secretion of differentiated 3T3-L1 adipocytes.
abstract: OBJECTIVE
  To investigate the effect of telmisartan on the lipometabolisms and the proinflammatory factors secreted from 3T3-L1 adipocytes and to explore the possible mechanisms.
  
  
  METHODS
  Telmisartan was applied to interfere with mature 3T3-L1 adipocytes. The culture's free fatty acids, interleukin 6 (IL-6) and tumor necrosis factor α (TNFα) were evaluated. Oil Red O staining was used to determine the adipogenesis of 3T3-L1 adipocytes. (18)F-FDG uptake levels corrected for protein content were determined by cellular radioactivity. The total RNA was isolated for hybridization experimentation in the microarray.
  
  
  RESULTS
  Telmisartan reduced lipid storage and increased (18)F-FDG uptake in a dose-dependent manner, reduced the levels of IL-6 and TNFα and increased those of free fatty acids. One hundred and fifty-seven differentially expressed genes were found by microarray. The mitogen-activated protein kinase (MAPK) signaling pathway involved in the secretion of proinflammatory factor and lipid metabolisms was affected by telmisartan. The expression of endothelial nitric oxide synthetase gene 3 (Nos3) and carnitine palmitoyl transferase 1α (CPT1α) was up-regulated by telmisartan.
  
  
  CONCLUSIONS
  Telmisartan affected lipometabolisms and the proinflammatory factors secreted from adipocytes. Nos3, CPT1α and the MAPK pathway being affected by telmisartan may be the underlying cause of the improvement in lipid metabolisms and secretion of proinflammatory factors of differentiated 3T3-L1 adipocytes.
authors.affiliation: Department of Endocrinology, PLA General Hospital, Beijing, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Kang
authors.affiliation: Department of Endocrinology, PLA General Hospital, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yijun
authors.affiliation: Department of Endocrinology, PLA General Hospital, Beijing, China jingtaodou@sohu.com.
authors.forename: Dou
authors.initials: D
authors.lastname: Jingtao
authors.affiliation: Department of Endocrinology, PLA General Hospital, Beijing, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Changyu
authors.affiliation: Department of Endocrinology, PLA General Hospital, Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wenhua
authors.affiliation: Department of Endocrinology, PLA General Hospital, Beijing, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Baoan
authors.affiliation: Department of Endocrinology, PLA General Hospital, Beijing, China.
authors.forename: Ma
authors.initials: M
authors.lastname: Fangling
authors.affiliation: Department of Endocrinology, PLA General Hospital, Beijing, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Xianling
authors.affiliation: Department of Endocrinology, PLA General Hospital, Beijing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Guoqing
authors.affiliation: Department of Endocrinology, PLA General Hospital, Beijing, China.
authors.forename: Mu
authors.initials: M
authors.lastname: Yiming
authors.affiliation: Department of Endocrinology, PLA General Hospital, Beijing, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Juming
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology*; D001565:Benzoates / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics; D019788:Fluorodeoxyglucose F18 / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism*; D066292:Lipid Droplets / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects* / Q000235:genetics; D020935:MAP Kinase Signaling System / Q000187:drug effects / Q000235:genetics; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction; D000077333:Telmisartan
chemical_list: D001562:Benzimidazoles; D001565:Benzoates; D018836:Inflammation Mediators; D019788:Fluorodeoxyglucose F18; D000077333:Telmisartan
keywords: Angiotensin 11 receptor antagonist; adipocyte; microarray; proinflammatory factor; telmisartan