title: The impact of antiandrogen 2-hydroxyflutamide on the expression of steroidogenic enzymes in cultured porcine ovarian follicles.
abstract: We used our model system for agonism and antagonism of the androgen receptor (AR), in which the porcine ovarian follicles were exposed on the excessive concentration of an AR agonist- testosterone (T) or an AR antagonist- 2-hydroxyflutamide (2-Hf) to: (1) analyze the spatiotemporal expression of ovarian 3β-hydroxysteroid dehydrogenase (3β-HSD), cytochrome P450 17α-hydroxylase/c17,20-lyase (P450c17) and cytochrome P450 aromatase (P450arom); (2) to determine the contribution of AR-mediated action during steroidogenesis and (3) to establish some correlations between the onset and expression pattern of the investigated proteins. Whole follicles (6-8 mm in diameter) isolated from mature porcine ovaries have been incubated (for 24 h) in an organ culture system in the presence of T (10(-7 )M), 2-Hf (1.7 × 10(-4) M) or both T and 2-hydroxyflutamide (T+2-Hf, at the same concentrations as when added separately). Thereafter, sections obtained from cultured follicles were processed for main steroidogenic enzymes detection by immunohistochemistry. Moreover, expression of their mRNA and protein was determined by real-time PCR and Western blot analysis. Progesterone, androgens and estradiol concentrations in the culture media were measured by radioimmunoassays (RIA). Our results demonstrated that 2-Hf can influence the steroidogenic activity of porcine follicles in vitro through the blockade of AR. It was shown that follicular 2-Hf treatment brought about dramatic decline in the production of the investigated steroids. What is more the addition of 2-Hf separately caused a negative effect on 3β-HSD and P450c17 mRNA and protein expression by ovarian follicles, while it was without effect on P450arom mRNA level. Quite opposite effect was observed in case of the simultaneous addition of 2-Hf and T. It caused high increase, in both P450arom mRNA and its protein. What was interesting, addition T+2-Hf evoked 3β-HSD and P450c17 increase on mRNA level, but decreased their protein expression. This was against our expectations but the reason for that finding remains undiscovered, intriguing and worth reporting. These results suggest that alike, steroidogenic enzymes activity and their expression is associated with the presence of androgens and AR in the porcine ovary.
authors.affiliation: Department of Endocrinology, Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387, Cracow, Poland, duda.m@op.pl.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Duda
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Grzesiak
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Knet
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Knapczyk-Stwora
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Tabarowski
authors.forename: Agata
authors.initials: A
authors.lastname: Michna
authors.forename: Maria
authors.initials: M
authors.lastname: Slomczynska
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics* / Q000378:metabolism; D000728:Androgens / Q000096:biosynthesis / Q000378:metabolism; D000818:Animals; D001141:Aromatase / Q000235:genetics* / Q000378:metabolism; D004958:Estradiol / Q000096:biosynthesis / Q000378:metabolism; D005260:Female; D005485:Flutamide / Q000031:analogs & derivatives* / Q000494:pharmacology; D005786:Gene Expression Regulation; D006080:Ovarian Follicle / Q000187:drug effects* / Q000378:metabolism; D011374:Progesterone / Q000096:biosynthesis / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013254:Steroid 17-alpha-Hydroxylase / Q000235:genetics* / Q000378:metabolism; D013552:Swine; D013739:Testosterone / Q000494:pharmacology*; D046509:Tissue Culture Techniques
chemical_list: D000728:Androgens; D012333:RNA, Messenger; D011944:Receptors, Androgen; C014290:hydroxyflutamide; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D005485:Flutamide; D015096:3-Hydroxysteroid Dehydrogenases; C477269:3 beta-hydroxy-delta 5-steroid dehydrogenase, rat; D001141:Aromatase; D013254:Steroid 17-alpha-Hydroxylase