title: Two pear glutathione S-transferases genes are regulated during fruit development and involved in response to salicylic acid, auxin, and glucose signaling.
abstract: Two genes encoding putative glutathione S-transferase proteins were isolated from pear (Pyrus pyrifolia) and designated PpGST1 and PpGST2. The deduced PpGST1 and PpGST2 proteins contain conserved Glutathione S-transferase N-terminal domain (GST_N) and Glutathione S-transferase, C-terminal domain (GST_C). Using PCR amplification technique, the genomic clones corresponding to PpGST1 and PpGST2 were isolated and shown to contain two introns and a singal intron respectively with typical GT/AG boundaries defining the splice junctions. Phylogenetic analysis clearly demonstrated that PpGST1 belonged to Phi class of GST superfamilies and had high homology with apple MdGST, while PpGST2 was classified into the Tau class of GST superfamilies. The expression of PpGST1 and PpGST2 genes was developmentally regulated in fruit. Further study demonstrated that PpGST1 and PpGST2 expression was remarkably induced by glucose, salicylic acid (SA) and indole-3-aceticacid (IAA) treatments in pear fruit, and in diseased fruit. These data suggested that PpGST1 and PpGST2 might be involved in response to sugar, SA, and IAA signaling during fruit development of pear.
authors.affiliation: College of Horticulture, Agricultural University of Hebei, Baoding, China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Shi
authors.affiliation: College of Horticulture, Agricultural University of Hebei, Baoding, China.
authors.forename: Zheng-Hong
authors.initials: ZH
authors.lastname: Li
authors.affiliation: College of Horticulture, Agricultural University of Hebei, Baoding, China.
authors.forename: Yu-Xing
authors.initials: YX
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture and Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.affiliation: College of Horticulture, Agricultural University of Hebei, Baoding, China.
authors.forename: Di-Ying
authors.initials: DY
authors.lastname: Xiang
authors.affiliation: College of Horticulture, Agricultural University of Hebei, Baoding, China.
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D005638:Fruit / Q000254:growth & development* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000502:physiology*; D015723:Gene Library; D005947:Glucose / Q000494:pharmacology; D005982:Glutathione Transferase / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D007210:Indoleacetic Acids / Q000494:pharmacology; D008969:Molecular Sequence Data; D010802:Phylogeny; D017434:Protein Structure, Tertiary / Q000235:genetics; D031989:Pyrus / Q000201:enzymology*; D060888:Real-Time Polymerase Chain Reaction; D020156:Salicylic Acid / Q000494:pharmacology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D017931:DNA Primers; D007210:Indoleacetic Acids; C030737:indoleacetic acid; D005982:Glutathione Transferase; D005947:Glucose; D020156:Salicylic Acid