title: An efficient covalent coating on glass slides for preparation of optical oligonucleotide microarrays.
abstract: OBJECTIVE
  Microarrays are potential analyzing tools for genomics and proteomics researches, which is in needed of suitable substrate for coating and also hybridization of biomolecules.
  
  
  METHODS
  In this research, a thin film of oxidized agarose was prepared on the glass slides which previously coated with poly-L-lysine (PLL). Some of the aldehyde groups of the activated agarose linked covalently to PLL amine groups; also bound to the amino groups of biomolecules. These linkages were fixed by UV irradiation. The prepared substrates were compared to only agarose-coated and PLL-coated slides.
  
  
  RESULTS
  RESULTS on atomic force microscope (AFM) demonstrated that agarose provided three-dimensional surface which had higher loading and bindig capacity for biomolecules than PLL-coated surface which had two-dimensional surface. In addition, the signal-to-noise ratio in hybridization reactions performed on the agarose-PLL coated substrates increased two fold and four fold compared to agarose and PLL coated substrates, respectively.
  
  
  CONCLUSIONS
  The agarose-PLL microarrays had the highest signal (2546) and lowest background signal (205) in hybridization, suggesting that the prepared slides are suitable in analyzing wide concentration range of analytes.
authors.affiliation: Biomaterials Group (Center of Excellence in Biomaterials), Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran ; Nanobiotechnology center, Department of Chemical Engineering, Jondishapour University of Technology, Dezful, Iran.
authors.forename: Atefeh
authors.initials: A
authors.lastname: Pourjahed
authors.affiliation: Biomaterials Group (Center of Excellence in Biomaterials), Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Rabiee
authors.affiliation: Biomaterials Group (Center of Excellence in Biomaterials), Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran.
authors.forename: Mohammadreza
authors.initials: M
authors.lastname: Tahriri
keywords: Agarose; Coating; Microarray; PLL; Signal-to-noise