title: Immunological markers that correlate with protection immunity against tularemia infection.
abstract: An efficient immune response to tularemia is dependent on a strong cell-mediated component. We tried to identify markers of cellular immune responses that indicate a vaccine efficacy against tularemia. BALB/c mice were immunized with mutant F. tularensis 15∆23A and/or F. tularensis 15 NIIEG strains and then were challenged i.n. with F. tularensis Schu. We compared the influence of F. tularensis antigens (tularinum) in vitro on production of IL-1, IL-5, IL-6, IL-17, IFN-γ, and TNF-α by splenocytes obtained from intact mice and mice immunized with mutant F. tularensis 15∆23A and/or F. tularensis 15 NIIEG strains. We also compared expression of CD28, CD154, TLR-2, and CD69 markers on CD4 and CD8 T-cells after activation with tularinum in vitro. We found that tularinum-induced CD4(+) T-cells increased TNF-α and IFN-γ synthesis and expression of CD69 only in group mice with high degree of post immunization protection against F. tularensis Schu challenge. Estimation of CD69 expression on CD3(+)CD4(+) cells and IFN-γ, TNF-α synthesis by CD4(+) T-lymphocytes could be useful for determination protect ability of antitularemia immunity.
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology, Moscow, Obolensk, Russia, 142279, firstova@obolensk.org.
authors.forename: Victoria V
authors.initials: VV
authors.lastname: Firstova
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Mokrievich
authors.forename: Vitalii M
authors.initials: VM
authors.lastname: Pavlov
authors.forename: Aleksey A
authors.initials: AA
authors.lastname: Gorbatov
authors.forename: Tatiana I
authors.initials: TI
authors.lastname: Kombarova
authors.forename: Sergey F
authors.initials: SF
authors.lastname: Biketov
authors.forename: Ivan A
authors.initials: IA
authors.lastname: Dyatlov
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology / Q000494:pharmacology; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000276:immunology; D015415:Biomarkers / Q000378:metabolism; D002478:Cells, Cultured; D005604:Francisella tularensis / Q000276:immunology*; D015870:Gene Expression; D007111:Immunity, Cellular; D007114:Immunization; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology; D007378:Interleukins / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000276:immunology; D014406:Tularemia / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology; D014613:Vaccines, Attenuated
chemical_list: D000942:Antigens, Bacterial; D015703:Antigens, CD; D001428:Bacterial Vaccines; D015415:Biomarkers; D007378:Interleukins; C493486:Tlr2 protein, mouse; D051195:Toll-Like Receptor 2; D014409:Tumor Necrosis Factor-alpha; D014613:Vaccines, Attenuated; D007371:Interferon-gamma