title: Isolation of antifungal compound from Paeonia suffruticosa and its antifungal mechanism.
abstract: OBJECTIVE
  To isolate antifungal compound from Paeonia suffruticosa, and to find the antifungal mechanisms by observing the ultrastructural modifications of yeasts in growth phase produced by 1,2,3,4,6-penta-O-galloyl-beta-D-glucose (PGG).
  
  
  METHODS
  Peony (Paeonia suffruticosa) root bark (PRB) was separated by solvent extraction and purified by high performance liquid chromatography (HPLC) method using analytical and preparative reversed phase C18 column on the basis of bio-assay method. In order to investigate the antifungal mechanism of PGG, Yeasts were submitted to different concentrations [3 × minimum inhibition concentration (MIC), 0.3 × MIC] for 1 h under constant stirring at 30 °C, and transmission electron microscopy was performed.
  
  
  RESULTS
  Based on the antifungal activity of PRB on Candida glabrata CBS138, the antifungal compound were isolated in ethyl acetate layer of PRB and identified as PGG by mass spectrometry, 1H nuclear magnetic resonance (NMR) analyses, with molecular weight of 940 and molecular formular as C41H32O26. Transmission electron microscopy showed that PGG degraded the cell wall envelope.
  
  
  CONCLUSIONS
  The results suggest that PGG may be responsible for the antifungal activity of PRB by disrupting the structure of cell wall directly.
authors.affiliation: Department of Infectious Diseases, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
authors.forename: Bao-en
authors.initials: BE
authors.lastname: Wang
authors.forename: Shu-wen
authors.initials: SW
authors.lastname: Zhang
authors.forename: Shu-min
authors.initials: SM
authors.lastname: Yang
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Ai-min
authors.initials: AM
authors.lastname: Ren
authors.forename: En-tong
authors.initials: ET
authors.lastname: Yi
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects / Q000648:ultrastructure; D002851:Chromatography, High Pressure Liquid; D047348:Hydrolyzable Tannins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D029596:Paeonia / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D066244:Proton Magnetic Resonance Spectroscopy
chemical_list: D000935:Antifungal Agents; D047348:Hydrolyzable Tannins; D010936:Plant Extracts; C435084:pentagalloylglucose