title: Anthelmintic activities of aporphine from Nelumbo nucifera Gaertn. cv. Rosa-plena against Hymenolepis nana.
abstract: Nelumbo nucifera Gaertn. cv. Rosa-plena (Nelumbonaceae), commonly known as lotus, is a perennial aquatic plant grown and consumed throughout Asia. All parts of N. nucifera have been used for various medicinal purposes in oriental medicine. From the leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena (an aquatic plant), liriodenine (1), lysicamine (2), (-)-anonaine (3), (-)-asimilobine (4), (-)-caaverine (5), (-)-N-methylasimilobine (6), (-)-nuciferine (7), (-)-nornuciferine (8), (-)-roemerine (9), 7-hydroxydehydronuciferine (10) and cepharadione B (11) were isolated and identification and anthelmintic activities of aporphine was evaluated against Anisakis simplex and Hymenolepis nana. This study found that the above constituents killed H. nana or reduced their spontaneous movements (oscillation/peristalsis). However, the above constituents at various concentrations demonstrated no larvicidal effect or ability to halt spontaneous parasite movement for 72 h against A. simplex, respectively. In addition, according to an assay of cestocidal activity against H. nana and nematocidal activity against A. simplex, we found that the above compounds showed greater lethal efficacy on H. nana than against A. simplex. Further investigation showed that these above constituents have effects against peroxyl radicals under cestocidal effect. Together, these findings suggest that these constituents of Nelumbo nucifera Gaertn. cv. Rosa-plena might be used as anthelmintic agents against H. nana.
authors.affiliation: Department of Parasitology, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan. rjlin@kmu.edu.tw.
authors.forename: Rong-Jyh
authors.initials: RJ
authors.lastname: Lin
authors.affiliation: Department of Medicinal and Applied Chemistry, College of Life Science, Kaohsiung Medical University, Kaohsiung 807, Taiwan. u97021112@cc.kmu.edu.tw.
authors.forename: Mei-Hsuan
authors.initials: MH
authors.lastname: Wu
authors.affiliation: Department of Parasitology, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan. u102800001@cc.kmu.edu.tw.
authors.forename: Yi-Hsuan
authors.initials: YH
authors.lastname: Ma
authors.affiliation: Department of Parasitology, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan. leyich@kmu.edu.tw.
authors.forename: Li-Yu
authors.initials: LY
authors.lastname: Chung
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, School of Medical and Health Sciences, Fooyin University, Ta-Liao District, Kaohsiung 83102, Taiwan. xx377@fy.edu.tw.
authors.forename: Chung-Yi
authors.initials: CY
authors.lastname: Chen
authors.affiliation: Department of Parasitology, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan. chmiye@kmu.edu.tw.
authors.forename: Chuan-Min
authors.initials: CM
authors.lastname: Yen
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D017163:Anisakis / Q000187:drug effects / Q000502:physiology; D000871:Anthelmintics / Q000302:isolation & purification / Q000494:pharmacology*; D001060:Aporphines / Q000302:isolation & purification / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D048189:Hymenolepis nana / Q000187:drug effects* / Q000502:physiology; D009068:Movement / Q000187:drug effects; D031653:Nelumbo / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D013997:Time Factors
chemical_list: D000470:Alkaloids; D000871:Anthelmintics; D001060:Aporphines; D004365:Drugs, Chinese Herbal; C081279:aporphine