title: Optical coherence tomography provides images similar to histology and allows the performance of extensive measurements of drug-eluting metal stents in animal ureters.
abstract: Optical coherence tomography (OCT) images and histology images of metal stents (MSs) inserted in animal ureters were compared, and the reliability of an OCT-based automated method for the performance of quantitative evaluation of ureteral MSs was evaluated. A zotarolimus-eluting metal stent (ZES) and a bare metal stent (BMS) were inserted in each ureter of ten pigs and six rabbits. OCT was performed in unobstructed stented ureters. Histopathologic examination of the stented ureters embedded in glycol-methacrylate took place. Quadrants of OCT images were compared to their respective histologic images by employing two independent observers who delineated different layers in the quadrants of OCT images and correlated them to the respective histologic quadrants. Manual (integrated OCT device software) and automated measurements of the OCT images using an automated strut detection method were compared. The observers highly agreed on the delineation of urothelium from the lamina propria and the lamina propria from the muscle layer of the ureteral wall. The algorithm measurements were similar to the manual measurements, and the algorithm proved to be reliable in the evaluation of ureteral MSs. Significantly higher endothelial hyperplasia of the BMSs in comparison to the ZESs was also quantitatively demonstrated by the strut detection method. OCT proved to be a reliable method for the evaluation of ureteral MSs. OCT provided images of the stented ureteral lumen similar to light microscopy quality. Measurements of the stented ureter are reliably performed by the automated strut detection method.
authors.affiliation: Department of Urology, University of Patras, Patras, Greece, pkallidonis@yahoo.com.
authors.forename: P
authors.initials: P
authors.lastname: Kallidonis
authors.forename: G C
authors.initials: GC
authors.lastname: Kagadis
authors.forename: P
authors.initials: P
authors.lastname: Kitrou
authors.forename: A
authors.initials: A
authors.lastname: Tsamandas
authors.forename: I
authors.initials: I
authors.lastname: Kyriazis
authors.forename: I
authors.initials: I
authors.lastname: Georgiopoulos
authors.forename: D
authors.initials: D
authors.lastname: Karnabatidis
authors.forename: S
authors.initials: S
authors.lastname: Tsantis
authors.forename: D
authors.initials: D
authors.lastname: Liourdi
authors.forename: A
authors.initials: A
authors.lastname: Al-Aown
authors.forename: E
authors.initials: E
authors.lastname: Liatsikos
mesh_terms: D000818:Animals; D054855:Drug-Eluting Stents; D005260:Female; D008670:Metals; D011817:Rabbits; D015203:Reproducibility of Results; D020123:Sirolimus / Q000008:administration & dosage / Q000031:analogs & derivatives*; D034421:Sus scrofa; D041623:Tomography, Optical Coherence; D014513:Ureter / Q000187:drug effects* / Q000473:pathology
chemical_list: D008670:Metals; C489443:zotarolimus; D020123:Sirolimus