title: Multiplex detection of nucleic acids using a low cost microfluidic chip and a personal glucose meter at the point-of-care.
abstract: A simple assay for multiplex DNA detection has been developed using a microfluidic chip and a personal glucose meter. By using this system, multiplex detection of three genotypes of hepatitis B virus DNA was possible with a detection limit of 10 pM. This point-of-care assay represented a versatile platform for sensitive multiplex target detection.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China. kmwang@hnu.edu.cn.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Xiaohai
authors.initials: X
authors.lastname: Yang
authors.forename: Kemin
authors.initials: K
authors.lastname: Wang
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhao
authors.forename: Pei
authors.initials: P
authors.lastname: Liu
authors.forename: Rongjuan
authors.initials: R
authors.lastname: Liu
mesh_terms: D015342:DNA Probes / Q000737:chemistry / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis*; D005838:Genotype; D006515:Hepatitis B virus / Q000235:genetics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009693:Nucleic Acid Hybridization; D019095:Point-of-Care Systems
chemical_list: D015342:DNA Probes; D004279:DNA, Viral