title: Therapeutic potential of morin against liver fibrosis in rats: modulation of oxidative stress, cytokine production and nuclear factor kappa B.
abstract: Therapeutic potential of morin, a member of flavonoid family, against carbon tetrachloride (CCl4)-induced liver fibrosis in rats was investigated and compared with that of silymarin. Results show that treatment with morin (30 mg/kg/day) revealed attenuation in liver index and serum biomarkers of liver function that were enhanced by chronic CCl4 intoxication. Further, morin inhibited the elevated levels of malondialdehyde and nitric oxide and restored hepatic reduced glutathione to its normal level. The increased production of hepatic hydroxyproline content by CCl4 was markedly decreased by administration of morin. In addition, treatment with morin significantly attenuated the inflammatory responses caused by CCl4 as evident by the decreased hepatic tumor necrosis factor-alpha (TNF-α) level, immunohistochemical expressions of inducible nitric oxide synthase and nuclear factor kappa B. Collectively, this study indicates that morin possesses antifibrotic effect in the CCl4 model of fibrosis via reducing oxidative stress, inflammatory responses and fibrogenic markers.
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Pharmacy, Minia University, El-Minia, Egypt. Electronic address: ghhh70@yahoo.com.
authors.forename: Gehan H
authors.initials: GH
authors.lastname: Heeba
authors.affiliation: Department of Agricultural Chemistry, Faculty of Agriculture, Minia University, El-Minia, Egypt.
authors.forename: Magda E
authors.initials: ME
authors.lastname: Mahmoud
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D002251:Carbon Tetrachloride; D005419:Flavonoids / Q000494:pharmacology / Q000627:therapeutic use*; D006909:Hydroxyproline / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D016328:NF-kappa B / Q000235:genetics; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D012838:Silymarin / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D047309:Flavones
chemical_list: D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D001219:Aspartate Aminotransferases; D001663:Bilirubin; D002251:Carbon Tetrachloride; D005419:Flavonoids; D006909:Hydroxyproline; D016328:NF-kappa B; D052247:Nitric Oxide Synthase Type II; D020011:Protective Agents; D012838:Silymarin; D014409:Tumor Necrosis Factor-alpha; C008548:morin; D047309:Flavones; C496318:Nos2 protein, rat
keywords: Carbon tetrachloride; Liver fibrosis; Morin; Nuclear factor kappa B; Oxidative stress; Silymarin