title: Expression of β-amyloid precursor protein in refractory epilepsy.
abstract: β-amyloid precursor protein (β-APP), also known as Aβ peptide, has a key role in the pathogenesis of Alzheimer's disease, and is also likely to be involved in the development of refractory epilepsy. The mechanism behind the association between β-APP and refractory epilepsy remains to be elucidated. The aim of the present study was to examine the levels of APP mRNA and β-APP protein in patients with refractory epilepsy. Tissue samples were obtained from patients with chronic pharmacoresistant epilepsy who underwent surgery. Levels of APP mRNA and β-APP protein in epileptic temporal lobe and hippocampal tissue were assessed using quantitative polymerase chain reaction, immunohistochemistry and immunofluorescence. The expression levels of protein significantly increased in the temporal cortex and the hippocampus of the patients with epilepsy. β-APP may thus contribute to the pathogenesis of refractory epilepsy.
authors.affiliation: Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Xiutian
authors.initials: X
authors.lastname: Sima
authors.affiliation: Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Neurology, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Jinmei
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Weiying
authors.initials: W
authors.lastname: Zhong
authors.affiliation: Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: You
mesh_terms: D000293:Adolescent; D000328:Adult; D016564:Amyloid beta-Protein Precursor / Q000378:metabolism*; D016022:Case-Control Studies; D004827:Epilepsy / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D008875:Middle Aged; D055815:Young Adult
chemical_list: D016564:Amyloid beta-Protein Precursor