title: Identifying proteins that bind a known RNA sequence using the yeast three-hybrid system.
abstract: The yeast three-hybrid system can be used to identify a protein partner of a known RNA sequence by screening a cDNA library fused to a transcription activation domain, with a hybrid RNA as 'bait.' Most commonly, such screens are performed to identify proteins that interact with a given RNA in vivo.
authors.affiliation: Microbiology Doctoral Training Program, University of Wisconsin - Madison, Madison, WI, USA.
authors.forename: Yvonne Y
authors.initials: YY
authors.lastname: Koh
authors.affiliation: Department of Biochemistry, University of Wisconsin - Madison, Madison, WI, USA. Electronic address: wickens@biochem.wisc.edu.
authors.forename: Marvin
authors.initials: M
authors.lastname: Wickens
mesh_terms: D000640:Amitrole / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology; D001483:Base Sequence; D015723:Gene Library; D017930:Genes, Reporter; D011485:Protein Binding; D012313:RNA / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000254:growth & development; D017422:Sequence Analysis, DNA; D014170:Transformation, Genetic; D020798:Two-Hybrid System Techniques; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
chemical_list: D000935:Antifungal Agents; D016601:RNA-Binding Proteins; D012313:RNA; D001616:beta-Galactosidase; D000640:Amitrole
keywords: 3-Aminotriazole (3-AT) concentration; Elimination of RNA-independent false positives; RNA plasmid; Transformation efficiency; Yeast three-hybrid system; cDNA library; β-Galactosidase activity