title: A planar Schiff base platinum(II) complex: crystal structure, cytotoxicity and interaction with DNA.
abstract: A new platinum(II) complex of salphen derivative, namely Schiff base ligand that derived from o-phenylenediamine and 5-chlorosalicylaldehyde was synthesized. The complex possessed a planar mononuclear structure. The in vitro cytotoxicities of the complex were evaluated by microculture tetrozolium (MTT) assay against seven human tumor cell lines with the IC50 values of ca. 11.61 µM. Cell cycle analysis indicated that the complex induced apoptosis and G1-phase arrest in A549 cells. The results of colony formation assay showed that the complex could suppress the proliferation and viability of A549 cells. The binding of the complex to potential target DNA were investigated by fluorescence spectroscopy, viscosity measurements, fluorescence polarization and agarose gel electrophoresis. The results suggest that the most probable binding mode of the complex is intercalation.
authors.affiliation: State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources,  School of Chemistry & Chemical Engineering of Guangxi Normal University.
authors.forename: Yan
authors.initials: Y
authors.lastname: Peng
authors.forename: Hui
authors.initials: H
authors.lastname: Zhong
authors.forename: Zhen-Feng
authors.initials: ZF
authors.lastname: Chen
authors.forename: Yan-Cheng
authors.initials: YC
authors.lastname: Liu
authors.forename: Guo-Hai
authors.initials: GH
authors.lastname: Zhang
authors.forename: Qi-Pin
authors.initials: QP
authors.lastname: Qin
authors.forename: Hong
authors.initials: H
authors.lastname: Liang
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D003460:Crystallization; D004247:DNA / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D009944:Organoplatinum Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D012545:Schiff Bases / Q000737:chemistry* / Q000494:pharmacology*; D014410:Tumor Stem Cell Assay
chemical_list: D000970:Antineoplastic Agents; D009944:Organoplatinum Compounds; D012545:Schiff Bases; D004247:DNA