title: Crotalus durissus collilineatus Venom Induces TNF- α and IL-10 Production in Human Peripheral Blood Mononuclear Cells.
abstract: Snake venom has been the subject of numerous studies in an attempt to find properties and biological effects that may be beneficial to man. In this study we evaluated in vitro the effects of Crotalus durissus terrificus (Cdt) and Crotalus durissus collilineatus (Cdc) venom in human peripheral blood mononuclear cells (PBMCs). At 24 h, a significant decrease of viable cells was observed in cells stimulated with the Cdc venom at 0.0005 mg/mL and 0.005 mg/mL compared to the negative control. At 48 h, a significant decrease of viable cells was observed only in cells stimulated with Cdc venom at 0.005 mg/mL. A significant increase of TNF- α and IL-10 was detected 48 hours after culture of PBMC with Cdc, but not with Cdt venom. The expression of CD69 and PD1 (programmed death-1), activation and regulatory cell markers, on CD8+ and CD8- T cells did not change in the presence of Cdt and Cdc venom. Our results suggest the presence of proinflammatory and anti-inflammatory components in the Cdc venom. Further analysis should be done to identify those Cdc venom components as it has been done for the Cdt venom by other authors, indicating that modulatory components are found in the venom of different species of Crotalus snakes.
authors.affiliation: Departamento de Biomedicina e Farmácia, Pontifícia Universidade Católica de Goiás, 74605-140 Goiania, GO, Brazil.
authors.forename: Camila Bastos
authors.initials: CB
authors.lastname: Ribeiro
authors.affiliation: Departamento de Biomedicina e Farmácia, Pontifícia Universidade Católica de Goiás, 74605-140 Goiania, GO, Brazil.
authors.forename: Jéssica Cristina
authors.initials: JC
authors.lastname: Dos Santos
authors.affiliation: Departamento de Biomedicina e Farmácia, Pontifícia Universidade Católica de Goiás, 74605-140 Goiania, GO, Brazil.
authors.forename: Jacyelle Medeiros
authors.initials: JM
authors.lastname: Silva
authors.affiliation: Departamento de Biomedicina e Farmácia, Pontifícia Universidade Católica de Goiás, 74605-140 Goiania, GO, Brazil.
authors.forename: Pedro Henrique Silva
authors.initials: PH
authors.lastname: de Godoi
authors.affiliation: Centro de Estudos e Pesquisas Biológicas (CEPB), Pontifícia Universidade Católica de Goiás, 74605-010 Goiania, GO, Brazil.
authors.forename: Marta Regina
authors.initials: MR
authors.lastname: Magalhães
authors.affiliation: Laboratório de Imunogenética, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
authors.forename: Mônica
authors.initials: M
authors.lastname: Spadafora-Ferreira
authors.affiliation: Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, 74605-050 Goiania, GO, Brazil.
authors.forename: Simone Gonçalves
authors.initials: SG
authors.lastname: Fonseca
authors.affiliation: Departamento de Biomedicina e Farmácia, Pontifícia Universidade Católica de Goiás, 74605-140 Goiania, GO, Brazil.
authors.forename: Irmtraut Araci Hoffman
authors.initials: IA
authors.lastname: Pfrimer