title: Microfluidic probe for single-cell analysis in adherent tissue culture.
abstract: Single-cell analysis provides information critical to understanding key disease processes that are characterized by significant cellular heterogeneity. Few current methods allow single-cell measurement without removing cells from the context of interest, which not only destroys contextual information but also may perturb the process under study. Here we present a microfluidic probe that lyses single adherent cells from standard tissue culture and captures the contents to perform single-cell biochemical assays. We use this probe to measure kinase and housekeeping protein activities, separately or simultaneously, from single human hepatocellular carcinoma cells in adherent culture. This tool has the valuable ability to perform measurements that clarify connections between extracellular context, signals and responses, especially in cases where only a few cells exhibit a characteristic of interest.
authors.affiliation: Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Aniruddh
authors.initials: A
authors.lastname: Sarkar
authors.affiliation: Department of Biological Engineering Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Sarah
authors.initials: S
authors.lastname: Kolitz
authors.affiliation: Department of Biological Engineering Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Douglas A
authors.initials: DA
authors.lastname: Lauffenburger
authors.affiliation: Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.|Department of Biological Engineering Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Jongyoon
authors.initials: J
authors.lastname: Han
mesh_terms: D056945:Hep G2 Cells; D006801:Humans; D044085:Microfluidics / Q000379:methods*; D059010:Single-Cell Analysis / Q000379:methods*