title: Effects of nonylphenol exposure on expression of cell receptors and secretory function in mouse Sertoli TM4 cells.
abstract: The aim of this study was to investigate the effects of nonylphenol (NP) exposure on the expression of cell receptors and secretory function in mouse Sertoli TM4 cells. There were no significant changes in mRNA expression of estrogen receptor (ER)-α and toll like receptor (TLR)-4 in the cells exposed to NP for 24h. However, the mRNA expression levels of ER-β, progesterone receptor (PR) and androgen receptor (AR) were down-regulated in NP groups. Furthermore, NP treatment evoked significant changes in protein expression levels of ER-β and follicle-stimulating hormone receptor (FSHR). There were significant changes in the mRNA expression levels of vinculin, N-cadherin and occludin, but not vimentin. Levels of inhibin B, androgen binding protein (ABP) and transferrin (Trf) were found to change significantly in NP challenged cells. Additionally, the decrease of nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) mRNA expression and increase of cytokine levels were simultaneously found in NP stimulated TM4 cells. In conclusion, these findings have shown that NP exposure affected expression of cell receptors and may damage specific secretory function of Sertoli TM4 cells, which may be associated with the male-specific reproductive toxicity of NP.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Xiaozhen
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Shaoping
authors.initials: S
authors.lastname: Nie
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Danfei
authors.initials: D
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address: myxie@ncu.edu.cn.
authors.forename: Mingyong
authors.initials: M
authors.lastname: Xie
mesh_terms: D000727:Androgen-Binding Protein / Q000378:metabolism; D000818:Animals; D054304:Anti-Mullerian Hormone / Q000235:genetics; D015820:Cadherins / Q000235:genetics; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D007265:Inhibins / Q000378:metabolism; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics; D062793:Occludin / Q000235:genetics; D010636:Phenols / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism; D011962:Receptors, FSH / Q000378:metabolism; D011987:Receptors, Steroid / Q000235:genetics; D012708:Sertoli Cells / Q000187:drug effects* / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000235:genetics; D014168:Transferrin / Q000235:genetics; D014746:Vimentin / Q000235:genetics; D016596:Vinculin / Q000235:genetics
chemical_list: D000727:Androgen-Binding Protein; D015820:Cadherins; C489083:Cdh2 protein, mouse; D016207:Cytokines; D062793:Occludin; C568986:Ocln protein, mouse; D010636:Phenols; D012333:RNA, Messenger; D011962:Receptors, FSH; D011987:Receptors, Steroid; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D014168:Transferrin; D014746:Vimentin; C422364:inhibin B; D016596:Vinculin; D009569:Nitric Oxide; D007265:Inhibins; C025256:nonylphenol; D054304:Anti-Mullerian Hormone; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse
keywords: Cell receptors; Nonylphenol; Secretory function; TM4 cells