title: Immobilisation of a fibrillin-1 fragment enhances the biocompatibility of PTFE.
abstract: Current vascular biomaterials exhibit poor biocompatibility characterised by failure to promote endothelialisation, predisposition to neoinitmal hyperplasia and excessive thrombogenicity. Fibrillin-1, a major constituent of microfibrils is associated with elastic fibres in the arterial wall. Fibrillin-1 binds to endothelial cells through an RGD cell adhesion motif in the fourth TB module. The RGD motif is present in PF8, a recombinant fibrillin-1 fragment. We investigated the potential of PF8 to improve the biocompatibility of PTFE. PF8 enhanced endothelial cell attachment and cell proliferation to a greater extent than fibronectin (p<0.01). PF8 immobilised on PTFE using plasma immersion ion implantation (PIII), retained these favourable cell interactive properties, again promoting endothelial cell attachment and proliferation. The thrombogenicity of covalently bound PF8 on PTFE was assessed in both static and dynamic conditions. In static conditions, uncoated PIII treated PTFE was more thrombogenic than untreated PTFE, while PF8 coating reduced thrombogenicity. Under flow, there was no difference in the thrombogenicity of PF8 coated PTFE and untreated PTFE. Immobilised PF8 shows a striking ability to promote attachment and growth of endothelial cells on PTFE, while providing a non-thrombogenic surface. These features make PF8 a promising candidate to improve the biocompatibility of current synthetic vascular grafts.
authors.affiliation: Heart Research Institute, Sydney, NSW 2042, Australia; The Baird Institute, Sydney, NSW 2042, Australia.
authors.forename: Hamid
authors.initials: H
authors.lastname: Hajian
authors.affiliation: Heart Research Institute, Sydney, NSW 2042, Australia; Sydney Medical School, University of Sydney, NSW 2006, Australia; School of Molecular Bioscience, University of Sydney, NSW 2006, Australia.
authors.forename: Steven G
authors.initials: SG
authors.lastname: Wise
authors.affiliation: School of Molecular Bioscience, University of Sydney, NSW 2006, Australia; Applied & Plasma Physics Group, Physics School, University of Sydney, NSW 2006, Australia.
authors.forename: Daniel V
authors.initials: DV
authors.lastname: Bax
authors.affiliation: Applied & Plasma Physics Group, Physics School, University of Sydney, NSW 2006, Australia.
authors.forename: Alexey
authors.initials: A
authors.lastname: Kondyurin
authors.affiliation: Wyss Institute, Harvard, Boston, MA 02115, USA.
authors.forename: Anna
authors.initials: A
authors.lastname: Waterhouse
authors.affiliation: Heart Research Institute, Sydney, NSW 2042, Australia; Sydney Medical School, University of Sydney, NSW 2006, Australia.
authors.forename: Louise L
authors.initials: LL
authors.lastname: Dunn
authors.affiliation: Wellcome Trust Centre for Cell-Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, UK.
authors.forename: Cay M
authors.initials: CM
authors.lastname: Kielty
authors.affiliation: Heart Research Institute, Sydney, NSW 2042, Australia.
authors.forename: Young
authors.initials: Y
authors.lastname: Yu
authors.affiliation: School of Molecular Bioscience, University of Sydney, NSW 2006, Australia; Bosch Institute, University of Sydney, NSW 2006, Australia; Charles Perkins Centre, University of Sydney, NSW 2006, Australia.
authors.forename: Anthony S
authors.initials: AS
authors.lastname: Weiss
authors.affiliation: Applied & Plasma Physics Group, Physics School, University of Sydney, NSW 2006, Australia.
authors.forename: Marcela M M
authors.initials: MM
authors.lastname: Bilek
authors.affiliation: The Baird Institute, Sydney, NSW 2042, Australia; Sydney Medical School, University of Sydney, NSW 2006, Australia.
authors.forename: Paul G
authors.initials: PG
authors.lastname: Bannon
authors.affiliation: Heart Research Institute, Sydney, NSW 2042, Australia; Department of Cardiology, Royal Prince Alfred Hospital, NSW 2050, Australia. Electronic address: mkcng@med.usyd.edu.au.
authors.forename: Martin K C
authors.initials: MK
authors.lastname: Ng
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D042783:Endothelial Cells / Q000737:chemistry*; D000071838:Fibrillin-1; D000071837:Fibrillins; D057809:HEK293 Cells; D006801:Humans; D008840:Microfilament Proteins / Q000737:chemistry*; D011138:Polytetrafluoroethylene / Q000737:chemistry*; D054392:Adipokines
chemical_list: D001672:Biocompatible Materials; D000071838:Fibrillin-1; D000071837:Fibrillins; D008840:Microfilament Proteins; D011138:Polytetrafluoroethylene; C000605722:FBN1 protein, human; D054392:Adipokines
keywords: Biocompatibility; Endothelialisation; Fibrillin-1; Polytetrafluoroethylene; Vascular grafts