title: Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe.
abstract: We present an ex vivo study of temporally and spectrally resolved autofluorescence in a total of 47 endoscopic excision biopsy/resection specimens from colon, using pulsed excitation laser sources operating at wavelengths of 375 nm and 435 nm. A paired analysis of normal and neoplastic (adenomatous polyp) tissue specimens obtained from the same patient yielded a significant difference in the mean spectrally averaged autofluorescence lifetime -570 ± 740 ps (p = 0.021, n = 12). We also investigated the fluorescence signature of non-neoplastic polyps (n = 6) and inflammatory bowel disease (n = 4) compared to normal tissue in a small number of specimens.
authors.affiliation: Photonics Group, Department of Physics, Imperial College London, Prince Consort Road, London, SW7 2AZ, UK ; Endoscopy Unit, Department of Gastroenterology, Charing Cross Hospital, Imperial College Healthcare NHS Trust, Fulham Palace Road, London, W6 8RF, UK ; These authors contributed equally to this work.
authors.forename: Sergio
authors.initials: S
authors.lastname: Coda
authors.affiliation: Photonics Group, Department of Physics, Imperial College London, Prince Consort Road, London, SW7 2AZ, UK ; These authors contributed equally to this work.
authors.forename: Alex J
authors.initials: AJ
authors.lastname: Thompson
authors.affiliation: Photonics Group, Department of Physics, Imperial College London, Prince Consort Road, London, SW7 2AZ, UK.
authors.forename: Gordon T
authors.initials: GT
authors.lastname: Kennedy
authors.affiliation: Photonics Group, Department of Physics, Imperial College London, Prince Consort Road, London, SW7 2AZ, UK ; Endoscopy Unit, Department of Gastroenterology, Charing Cross Hospital, Imperial College Healthcare NHS Trust, Fulham Palace Road, London, W6 8RF, UK.
authors.forename: Kim L
authors.initials: KL
authors.lastname: Roche
authors.affiliation: Endoscopy Unit, Department of Gastroenterology, Charing Cross Hospital, Imperial College Healthcare NHS Trust, Fulham Palace Road, London, W6 8RF, UK.
authors.forename: Lakshmana
authors.initials: L
authors.lastname: Ayaru
authors.affiliation: Endoscopy Unit, Department of Gastroenterology, Charing Cross Hospital, Imperial College Healthcare NHS Trust, Fulham Palace Road, London, W6 8RF, UK.
authors.forename: Devinder S
authors.initials: DS
authors.lastname: Bansi
authors.affiliation: Department of Histopathology, Imperial College London, Du Cane Road, London, W12 0NN, UK ; Cancer Research UK London Research Institute, 44 Lincoln's Inn Fields, London, WC2A 3LY, UK.
authors.forename: Gordon W
authors.initials: GW
authors.lastname: Stamp
authors.affiliation: Photonics Group, Department of Physics, Imperial College London, Prince Consort Road, London, SW7 2AZ, UK ; Endoscopy Unit, Department of Gastroenterology, Charing Cross Hospital, Imperial College Healthcare NHS Trust, Fulham Palace Road, London, W6 8RF, UK ; These authors contributed equally to this work.
authors.forename: Andrew V
authors.initials: AV
authors.lastname: Thillainayagam
authors.affiliation: Photonics Group, Department of Physics, Imperial College London, Prince Consort Road, London, SW7 2AZ, UK ; These authors contributed equally to this work.
authors.forename: Paul M W
authors.initials: PM
authors.lastname: French
authors.affiliation: Photonics Group, Department of Physics, Imperial College London, Prince Consort Road, London, SW7 2AZ, UK ; Department of Histopathology, Imperial College London, Du Cane Road, London, W12 0NN, UK ; These authors contributed equally to this work.
authors.forename: Chris
authors.initials: C
authors.lastname: Dunsby
keywords: (120.3890) Medical optics instrumentation; (170.2680) Gastrointestinal; (170.3650) Lifetime-based sensing; (300.6500) Spectroscopy, time-resolved; (300.6540) Spectroscopy, ultraviolet; (300.6550) Spectroscopy, visible