title: Starch and lipid accumulation in eight strains of six Chlorella species under comparatively high light intensity and aeration culture conditions.
abstract: The microalgae family Chlorella species are known to accumulate starch and lipids. Although nitrogen or phosphorous deficiencies promote starch and lipids formation in many microalgae, these deficiencies also limit their growth and productivity. Therefore, the Chlorellaceae strains were attempted to increase starch and lipids productivity under high-light-intensity conditions (600-μmol photons m(-2)s(-1)). The 12:12-h light-dark (LD) cycle conditions elicited more stable growth than the continuous light (LL) conditions, whereas the starch and lipids yields increased in LL conditions. The amount of starch and lipids per cell increased in Chlorella viscosa and Chlorella vulgaris in sulfur-deficient medium, and long-chain fatty acids with 20 or more carbon atoms accumulated in cells grown in sulfur-deficient medium. Accumulation of starch and lipids was investigated in eight strains. The accumulation was strain-dependent, and varied according to the medium and light conditions. Five of the eight Chlorella strains exhibited similar accumulation patterns.
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Takeshita
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan; Japan Science and Technology Agency, CREST, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Ota
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan; Japan Science and Technology Agency, CREST, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan.
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Yamazaki
authors.affiliation: Bioimaging Center, Graduate School of Frontier Science, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan.
authors.forename: Aiko
authors.initials: A
authors.lastname: Hirata
authors.affiliation: Laboratory of the Cell Cycles of Algae, Institute of Microbiology, Academy of Sciences of the Czech Republic, Opatovický mlýn, Třeboň 379 81, Czech Republic.
authors.forename: Vilém
authors.initials: V
authors.lastname: Zachleder
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan; Japan Science and Technology Agency, CREST, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan. Electronic address: kawano@k.u-tokyo.ac.jp.
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Kawano
mesh_terms: D000388:Air; D002708:Chlorella / Q000145:classification / Q000254:growth & development / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D008027:Light; D050356:Lipid Metabolism; D013045:Species Specificity; D013213:Starch / Q000378:metabolism*; D013455:Sulfur / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D013455:Sulfur; D013213:Starch
keywords: Accumulation shift; Chlorella; Lipid; Starch; Strong light