title: Metabolic status of pluripotent cells and exploitation for growth in stirred suspension bioreactors.
abstract: Pluripotent stem cells are of great interest in the field of regenerative medicine. Recent studies have shown that they maintain a glycolytic metabolic status while pluripotent and wholesale changes to mitochondrial and metabolic profile occur during differentiation. This article reviews the process and how this may be exploited in a stirred suspension bioreactor for rapid growth while maintaining pluripotency.
authors.affiliation: a Department of Biochemistry and Molecular Biology, Faculty of Medical Science , University of Calgary , Calgary , Alberta , Canada.
authors.forename: Brad
authors.initials: B
authors.lastname: Day
authors.forename: Derrick E
authors.initials: DE
authors.lastname: Rancourt
mesh_terms: D019149:Bioreactors; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D006801:Humans; D008928:Mitochondria / Q000378:metabolism*; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D013535:Suspensions
chemical_list: D013535:Suspensions