title: The pqqC gene is essential for antifungal activity of Pseudomonas kilonensis JX22 against Fusarium oxysporum f. sp. lycopersici.
abstract: Strain JX22, exhibiting a broad range of antimicrobial activities to fungal pathogens, was isolated and classified as representing Pseudomonas kilonensis. In this study, the mutant JX22MT1 was obtained by the EZ-Tn5 transposon mutation and showed no antifungal activity against Fusarium oxysporum f. sp. lycopersici as compared with wild-type strain JX22. The pqqC gene was disrupted in the mutant. Antifungal activity at the wild-type level was restored from the mutant JX22MT1 with the introduction of the functional pqqC gene, which encodes pyrroloquinoline-quinone synthesis protein C. The results suggest that pqqC is essential for antifungal activity of P. kilonensis JX22 against F. oxysporum f. sp. lycopersici.
authors.affiliation: Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality, Ministry of Agriculture/Jiangsu Academy of Agricultural Sciences, Nanjing, China; Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, MS, USA.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Xu
authors.forename: Peng
authors.initials: P
authors.lastname: Deng
authors.forename: Kurt C
authors.initials: KC
authors.lastname: Showmaker
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Sonya M
authors.initials: SM
authors.lastname: Baird
authors.forename: Shi-En
authors.initials: SE
authors.lastname: Lu
mesh_terms: D000898:Antibiosis; D000935:Antifungal Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005670:Fusarium / Q000187:drug effects* / Q000254:growth & development; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010802:Phylogeny; D011549:Pseudomonas / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D000935:Antifungal Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; C095467:PqqC protein, Bacteria; D012336:RNA, Ribosomal, 16S
keywords: antimicrobial activity; bacteria; fungi; mutagenesis