title: Mechanisms of the 14-3-3 protein function: regulation of protein function through conformational modulation.
abstract: Many aspects of protein function regulation require specific protein-protein interactions to carry out the exact biochemical and cellular functions. The highly conserved members of the 14-3-3 protein family mediate such interactions and through binding to hundreds of other proteins provide multitude of regulatory functions, thus playing key roles in many cellular processes. The 14-3-3 protein binding can affect the function of the target protein in many ways including the modulation of its enzyme activity, its subcellular localization, its structure and stability, or its molecular interactions. In this minireview, we focus on mechanisms of the 14-3-3 protein-dependent regulation of three important 14-3-3 binding partners: yeast neutral trehalase Nth1, regulator of G-protein signaling 3 (RGS3), and phosducin.
authors.affiliation: Department of Protein Structure, Institute of Physiology Academy of Sciences of the Czech Republic, Prague, Czech Republic. obsilova@biomed.cas.cz and obsil@natur.cuni.cz.
authors.forename: V
authors.initials: V
authors.lastname: Obsilova
authors.forename: M
authors.initials: M
authors.lastname: Kopecka
authors.forename: D
authors.initials: D
authors.lastname: Kosek
authors.forename: M
authors.initials: M
authors.lastname: Kacirova
authors.forename: S
authors.initials: S
authors.lastname: Kylarova
authors.forename: L
authors.initials: L
authors.lastname: Rezabkova
authors.forename: T
authors.initials: T
authors.lastname: Obsil
mesh_terms: D048948:14-3-3 Proteins / Q000737:chemistry* / Q000648:ultrastructure*; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D045647:DNA Glycosylases / Q000737:chemistry* / Q000648:ultrastructure; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000737:chemistry* / Q000648:ultrastructure; D005136:Eye Proteins / Q000737:chemistry* / Q000648:ultrastructure; D020555:GTP-Binding Protein Regulators / Q000737:chemistry* / Q000648:ultrastructure; D006801:Humans; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry* / Q000648:ultrastructure; D010750:Phosphoproteins / Q000737:chemistry* / Q000648:ultrastructure; D011485:Protein Binding; D011487:Protein Conformation; D020710:RGS Proteins / Q000737:chemistry* / Q000648:ultrastructure; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry* / Q000648:ultrastructure; D013329:Structure-Activity Relationship
chemical_list: D048948:14-3-3 Proteins; D005136:Eye Proteins; D020555:GTP-Binding Protein Regulators; D009097:Multienzyme Complexes; C492078:Nth1 protein, S pombe; D010750:Phosphoproteins; D020710:RGS Proteins; C485898:RGS3 protein, human; D029702:Schizosaccharomyces pombe Proteins; C065631:phosducin; D045647:DNA Glycosylases; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase