title: Improved conditions for periodate/Schiff's base-based fluorescent staining of glycoproteins with dansylhydrazine in SDS-PAGE.
abstract: An improved periodate/Schiff's base based fluorescent stain with dansylhydrazine (DH) for glycoproteins in 1D and 2D SDS-PAGE was described. Down to 4-8 ng of glycoproteins can be selectively detected within 2 h, which is approximately 16-fold higher than that of original protocol, but similar to that of Pro-Q Emerald 488 stain (Invitrogen, Carlsbad, USA). Furthermore, subsequent study of deglycosylation, glycoprotein affinity isolation, and LC-MS/MS analysis were performed to confirm the specificity of the improved method. As a result, improved DH stain may provide a new choice for selective, economic, MS compatible, and convenient visualization of gel-separated glycoproteins.
authors.affiliation: Zhejiang Provincial Key Laboratory of Biopharmaceuticals, Wenzhou Medical University, Wenzhou, Zhejiang, P. R. China; Ningbo First Hospital, Ningbo, Zhejiang, P. R. China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhou
authors.forename: Guo-Ying
authors.initials: GY
authors.lastname: Hong
authors.forename: Bin-Bin
authors.initials: BB
authors.lastname: Huang
authors.forename: Yuan-Meng
authors.initials: YM
authors.lastname: Duan
authors.forename: Jia-Yi
authors.initials: JY
authors.lastname: Shen
authors.forename: Mao-Wei
authors.initials: MW
authors.lastname: Ni
authors.forename: Wei-Tao
authors.initials: WT
authors.lastname: Cong
authors.forename: Li-Tai
authors.initials: LT
authors.lastname: Jin
mesh_terms: D002846:Chromatography, Affinity; D003619:Dansyl Compounds / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D005456:Fluorescent Dyes / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry*; D006834:Hydrazines / Q000737:chemistry*; D010504:Periodic Acid / Q000737:chemistry*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry
chemical_list: D003619:Dansyl Compounds; D005456:Fluorescent Dyes; D006023:Glycoproteins; D006834:Hydrazines; D010504:Periodic Acid; C027913:dansyl hydrazine
keywords: Dansylhydrazine; Fluorescent stain; Glycoprotein detection