title: Re-examining class-I presentation and the DRiP hypothesis.
abstract: MHC class I molecules present peptides derived from intracellular proteins, enabling immune surveillance by CD8(+) T cells and the elimination of virus-infected and cancerous cells. It has been argued that the dominant source of MHC class I-presented peptides is through proteasomal degradation of newly synthesized defective proteins, termed defective ribosomal products (DRiPs). Here, we critically examine the DRiP hypothesis and discuss recent studies indicating that antigenic peptides are generated from the entire proteome and not just from failures in protein synthesis or folding.
authors.affiliation: Department of Pathology, University of Massachusetts Medical School, Worcester, Massachusetts, USA. Electronic address: Kenneth.Rock@umassmed.edu.
authors.forename: Kenneth L
authors.initials: KL
authors.lastname: Rock
authors.affiliation: Department of Pathology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
authors.forename: Diego J
authors.initials: DJ
authors.lastname: Farfán-Arribas
authors.affiliation: Department of Pathology, University of Massachusetts Medical School, Worcester, Massachusetts, USA. Electronic address: jeff.colbert@umassmed.edu.
authors.forename: Jeff D
authors.initials: JD
authors.lastname: Colbert
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Alfred L
authors.initials: AL
authors.lastname: Goldberg
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D007157:Immunologic Surveillance / Q000276:immunology*; D010455:Peptides / Q000276:immunology; D020543:Proteome / Q000276:immunology; D012270:Ribosomes / Q000276:immunology
chemical_list: D015395:Histocompatibility Antigens Class I; D010455:Peptides; D020543:Proteome
keywords: MHC class I; antigen presentation; defective ribosomal product; proteasome