title: Organochlorine pesticides and antioxidant enzymes are inversely correlated with liver enzyme gene expression in Cyprinus carpio.
abstract: The present study was designed to investigate the association between levels of organochlorine pesticides (OCPs) and liver enzyme responses in Cyprinus carpio. Fish were caught at three stations in the Büyük Menderes River (BMR): the origin, the Sarayköy station, and the estuary. Seventeen OCPs were quantified in liver tissue, as well as in river water by gas chromatography (GC)-electron capture detection, and structures were confirmed by negative chemical ionization-GC-mass spectrometry. The activities of CYP1A, GST, Se-GPx, CAT, and SODs were determined by spectrophotometry or fluorimetry. The mRNA levels of CYP1A, GST, and SOD1 were quantified by real-time RT-PCR. CYP1A and antioxidant enzyme activities were dramatically higher at the Sarayköy station, where OCP pollution is higher than the other two stations. Mn-SOD is responsible for the increase in total SOD activity in the Sarayköy samples. However, gene expression levels of certain enzymes were heavily suppressed. Our findings show that the transcriptional and functional responses of CYP1A and antioxidant enzymes are inversely correlated.
authors.affiliation: Department of Toxicology, Faculty of Pharmacy, Ege University, 35100 Bornova, İzmir, Turkey.
authors.forename: Melis
authors.initials: M
authors.lastname: Karaca
authors.affiliation: Department of Bioengineering, Faculty of Engineering, Ege University, 35100 Bornova, İzmir, Turkey.
authors.forename: Lokman
authors.initials: L
authors.lastname: Varışlı
authors.affiliation: Department of Bioengineering, Faculty of Engineering, Ege University, 35100 Bornova, İzmir, Turkey.
authors.forename: Kemal
authors.initials: K
authors.lastname: Korkmaz
authors.affiliation: Department of Hydrobiology, Ege University, 35100 Bornova, İzmir, Turkey.
authors.forename: Okan
authors.initials: O
authors.lastname: Özaydın
authors.affiliation: Department of Aquaculture, Faculty of Fisheries, Ege University, 35100 Bornova, İzmir, Turkey.
authors.forename: Fatih
authors.initials: F
authors.lastname: Perçin
authors.affiliation: Department of Toxicology, Faculty of Pharmacy, Ege University, 35100 Bornova, İzmir, Turkey. Electronic address: hilmi.orhan@ege.edu.tr.
authors.forename: Hilmi
authors.initials: H
authors.lastname: Orhan
mesh_terms: D000818:Animals; D002347:Carps; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics; D006843:Hydrocarbons, Chlorinated / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D010575:Pesticides / Q000633:toxicity*; D012333:RNA, Messenger / Q000032:analysis; D013482:Superoxide Dismutase / Q000235:genetics*
chemical_list: D006843:Hydrocarbons, Chlorinated; D010575:Pesticides; D012333:RNA, Messenger; D019363:Cytochrome P-450 CYP1A1; D013482:Superoxide Dismutase; D005982:Glutathione Transferase
keywords: Antioxidant enzyme; Büyük Menderes River; CYP1A; Cyprinus carpio; Gene expression; Organochlorine pesticide