title: Investigation of intercellular salicylic acid accumulation during compatible and incompatible Arabidopsis-pseudomonas syringae interactions using a fast neutron-generated mutant allele of EDS5 identified by genetic mapping and whole-genome sequencing.
abstract: A whole-genome sequencing technique developed to identify fast neutron-induced deletion mutations revealed that iap1-1 is a new allele of EDS5 (eds5-5). RPS2-AvrRpt2-initiated effector-triggered immunity (ETI) was compromised in iap1-1/eds5-5 with respect to in planta bacterial levels and the hypersensitive response, while intra- and intercellular free salicylic acid (SA) accumulation was greatly reduced, suggesting that SA contributes as both an intracellular signaling molecule and an antimicrobial agent in the intercellular space during ETI. During the compatible interaction between wild-type Col-0 and virulent Pseudomonas syringae pv. tomato (Pst), little intercellular free SA accumulated, which led to the hypothesis that Pst suppresses intercellular SA accumulation. When Col-0 was inoculated with a coronatine-deficient strain of Pst, high levels of intercellular SA accumulation were observed, suggesting that Pst suppresses intercellular SA accumulation using its phytotoxin coronatine. This work suggests that accumulation of SA in the intercellular space is an important component of basal/PAMP-triggered immunity as well as ETI to pathogens that colonize the intercellular space.
authors.affiliation: McMaster University, Department of Biology, Hamilton, Ontario, Canada.
authors.forename: Jessie L
authors.initials: JL
authors.lastname: Carviel
authors.affiliation: McMaster University, Department of Biology, Hamilton, Ontario, Canada.
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Wilson
authors.affiliation: McMaster University, Department of Biology, Hamilton, Ontario, Canada.
authors.forename: Marisa
authors.initials: M
authors.lastname: Isaacs
authors.affiliation: McMaster University, Department of Biology, Hamilton, Ontario, Canada.
authors.forename: Philip
authors.initials: P
authors.lastname: Carella
authors.affiliation: McMaster University, Department of Biology, Hamilton, Ontario, Canada.
authors.forename: Vasile
authors.initials: V
authors.lastname: Catana
authors.affiliation: McMaster University, Department of Biology, Hamilton, Ontario, Canada.
authors.forename: Brian
authors.initials: B
authors.lastname: Golding
authors.affiliation: McMaster University, Department of Biology, Hamilton, Ontario, Canada.
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Weretilnyk
authors.affiliation: McMaster University, Department of Biology, Hamilton, Ontario, Canada.
authors.forename: Robin K
authors.initials: RK
authors.lastname: Cameron
mesh_terms: D000483:Alleles; D000596:Amino Acids / Q000494:pharmacology; D017360:Arabidopsis / Q000235:genetics / Q000276:immunology / Q000382:microbiology*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D016923:Cell Death; D002874:Chromosome Mapping; D060467:Disease Resistance; D004573:Electrolytes / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism; D005214:Fast Neutrons; D018745:Genome, Plant; D054884:Host-Pathogen Interactions / Q000235:genetics*; D007192:Indenes / Q000494:pharmacology; D026901:Membrane Transport Proteins / Q000235:genetics*; D009154:Mutation / Q000235:genetics*; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology; D057865:Plant Immunity; D044224:Pseudomonas syringae / Q000502:physiology*; D020156:Salicylic Acid / Q000378:metabolism*; D017422:Sequence Analysis, DNA
chemical_list: D000596:Amino Acids; D029681:Arabidopsis Proteins; C455514:EDS5 protein, Arabidopsis; D004573:Electrolytes; D007192:Indenes; D026901:Membrane Transport Proteins; C089426:RPS2 protein, Arabidopsis; C058487:coronatine; D020156:Salicylic Acid