title: Anti-inflammatory and analgesic activity of a cucurbitacin isolated from Lagenaria breviflora Roberty fruit.
abstract: BACKGROUND
  In this study, the bioactive compound in Lagenaria breviflora Roberty responsible for its anti-inflammatory and analgesic activities was isolated and chemically characterized.
  
  
  METHODS
  Compounds in the whole fruit, bark, pulp and seed of L. breviflora were partitioned utilizing their various polarity in n-hexane, ethyl acetate, chloroform and ethanol. The fractions of the extract obtained were tested for their bioactivities. The fraction with the most consistent anti-inflammatory and analgesic activities was further purified using accelerated gradient chromatography (AGC) and open column chromatography. Elution of compounds in this fraction was monitored through the different chromatography methods using thin layer chromatography (TLC). The pure compound isolated from the chromatography methods was taken for chemical characterization and elucidation of the structure.
  
  
  RESULTS
  Ethyl acetate fraction of the whole fruit exhibited the most consistent anti-inflammatory and analgesic activities out of the 16 fractions obtained. Purification of this fraction with AGC yielded 7 subfractions composing of eluents with similar Rf values on the TLC plate. One of the sub-fractions yielded a compound which was further purified using the open column chromatography method. Eluent obtained from this sub-fraction was renamed YO1.
  
  
  CONCLUSIONS
  From the result of mass spectroscopy and nuclear magnetic resonance spectroscopy of the compound, the structure of YO1 was determined as a cucurbitacin with 10á-cucurbit-5-ene skeleton (9â-methyl-19-norlanosta 5-ene) backbone structure, with six carbon atoms attached to double bonds and one hydroxyl group.
authors.affiliation: Department of Veterinary Physiology, Biochemistry and Pharmacology, University of lbadan, Ibadan, Nigeria. oa.oridupa@mail.ui.edu.ng
authors.forename: O A
authors.initials: OA
authors.lastname: Oridupa
authors.affiliation: Department of Veterinary Physiology, Biochemistry and Pharmacology, University of lbadan, Ibadan, Nigeria.
authors.forename: A B
authors.initials: AB
authors.lastname: Saba
authors.affiliation: Department of Chemistry, Obafemi Awolowo University, Ile-lfe, Nigeria.
authors.forename: J K
authors.initials: JK
authors.lastname: Adesanwo
authors.affiliation: Department of Animal Science, Obafemi Awolowo University, Ile-lfe, Nigeria.
authors.forename: B O
authors.initials: BO
authors.lastname: Oyebanji
mesh_terms: D000085:Acetates / Q000032:analysis* / Q000627:therapeutic use; D000818:Animals; D002855:Chromatography, Thin Layer; D019662:Cucurbitaceae / Q000737:chemistry*; D004195:Disease Models, Animal; D005260:Female; D005638:Fruit / Q000737:chemistry; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D010146:Pain / Q000188:drug therapy*; D010936:Plant Extracts / Q000737:chemistry* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000085:Acetates; D010936:Plant Extracts; C007650:ethyl acetate