title: Toxic effects of lead on biochemical and histological alterations in green mussel (Perna viridis) induced by environmentally relevant concentrations.
abstract: Acute and chronic toxicity tests were conducted on green mussel (Perna viridis) to determine the adverse effects of lead (Pb). Exposure of organisms to acute toxicity test for 96 h and lethal concentration (LC(50)) was the endpoint of the test. Acute toxicity for 96-h LC(50) and 95% confidence intervals of P. viridis was 2.62 ± 0.12 (2.62-3.24) mg/L Pb. Chronic toxicity tests revealed that survival of exposed organisms decreased with elevated exposure concentrations. No-observed-effect concentration (NOEC) and lowest-observed-effect concentration (LOEC) were calculated based on survival of test organisms. Results of this study demonstrated an increase in toxicity in test organisms with rise in exposure time and concentration. In this study, histology and biochemical enzymes, namely, catalase, reduced glutathione, glutathione S-transferase, and lipid peroxides, were correlated with chronic value and survival endpoints of P. viridis after chronic exposure to Pb. Biochemical and histological responses to different concentrations of Pb were assessed and significant differences were observed between control and increasing exposure concentrations. Biomarker studies in internal organs confirmed that the observed changes are due to adverse effects of Pb. This assessment of toxicity was the first step to determining the seawater quality criteria for marine organisms.
authors.affiliation: a Institute for Ocean Management , Anna University Chennai , Chennai , Tamil Nadu , India.
authors.forename: G
authors.initials: G
authors.lastname: Hariharan
authors.forename: R
authors.initials: R
authors.lastname: Purvaja
authors.forename: R
authors.initials: R
authors.lastname: Ramesh
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D007854:Lead / Q000633:toxicity*; D007928:Lethal Dose 50; D009132:Muscles / Q000187:drug effects; D049883:Perna / Q000187:drug effects*; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical; D007854:Lead