title: Efficient in vivo gene delivery using modified Tat peptide with cationic lipids.
abstract: A combination of modified HIV-1 Tat (mTat) peptide and cationic lipids, FuGENE HD (FH), dramatically enhanced transfection efficiency across a range of cell lines when compared to mTat or FH alone (Biomaterials 35:1705-1715 2014). The efficiency of this Tat peptide combination was significantly higher than many commercial non-viral vectors. In this present study, we tested the feasibility of this non-viral vector, mTat/FH, in vivo using plasmid DNA encoding a luciferase gene. The results of the in vivo studies showed that animals administered mTat/FH/DNA intramuscularly had significantly higher and longer luciferase expression (≈7 months) than those with mTat/DNA, FH/DNA, or DNA alone. Histological evaluation showed little immune response in the muscles, livers, and kidneys of mice administered with the mTat/FH. The combination of mTat with FH could significantly improve transfection efficiency, expanding the potential use of non-viral gene vectors in vivo.
authors.affiliation: Department of Prosthodontics, New York University College of Dentistry, 345 East 24th Street, 4W, New York, NY, 10010, USA, sy23@nyu.edu.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Yamano
authors.forename: Jisen
authors.initials: J
authors.lastname: Dai
authors.forename: Shigeru
authors.initials: S
authors.lastname: Hanatani
authors.forename: Ken
authors.initials: K
authors.lastname: Haku
authors.forename: Takuto
authors.initials: T
authors.lastname: Yamanaka
authors.forename: Mika
authors.initials: M
authors.lastname: Ishioka
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Takayama
authors.forename: Amr M
authors.initials: AM
authors.lastname: Moursi
mesh_terms: D000818:Animals; D017930:Genes, Reporter; D050356:Lipid Metabolism; D008156:Luciferases / Q000032:analysis / Q000235:genetics; D051379:Mice; D010957:Plasmids / Q000378:metabolism; D014162:Transfection / Q000379:methods*; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000378:metabolism*
chemical_list: D054322:tat Gene Products, Human Immunodeficiency Virus; D008156:Luciferases