title: Synergistic effects of loxoprofen and glycine on the micturition reflex in conscious rats.
abstract: We examined the inhibitory effects of loxoprofen, a cyclooxygenase inhibitor, and glycine, a major inhibitory neurotransmitter, on the micturition reflex in conscious rats and hypothesized that these drugs would interact synergistically to inhibit micturition. Voiding behaviors were assessed using a metabolic cage. Oral loxoprofen decreased the urinary frequency, and only a high dose(10 mg/kg) significantly reduced the voided volume. With cystometry, intravenous loxoprofen(0.1-3 mg/kg) and glycine (30 and 100 mg/kg) prolonged the intercontraction intervals (ICI) in adose-dependent manner, but did not change the maximum voiding pressure (MVP) in conscious rats. The combination of loxoprofen (3 mg/kg) and glycine (100 mg/kg) strongly prolonged the ICI more than with either drug alone. The lowest dose of loxoprofen (0.1 mg/kg) and glycine(30 mg/kg) did not affect either the ICI or the MVP, but their combination resulted in a significant increase in the ICI. These results suggest that the combined administration of loxoprofen and glycine produced a synergistic inhibitory effect on the micturition reflex.
authors.affiliation: Department of Neurophysiology, Akita University Graduate School of Medicine 1-1-1 Hondo, Akita, Akita 010-8543, Japan.
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Fukiya
authors.forename: Masaru
authors.initials: M
authors.lastname: Yoshizumi
authors.forename: Mikako
authors.initials: M
authors.lastname: Saito
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Matsumoto-Miyai
authors.forename: Toshie
authors.initials: T
authors.lastname: Nimura
authors.forename: Masahito
authors.initials: M
authors.lastname: Kawatani
mesh_terms: D000818:Animals; D003243:Consciousness; D004305:Dose-Response Relationship, Drug; D004333:Drug Administration Routes; D004357:Drug Synergism; D005260:Female; D005998:Glycine / Q000008:administration & dosage / Q000494:pharmacology*; D010666:Phenylpropionates / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D012018:Reflex / Q000187:drug effects* / Q000502:physiology*; D014554:Urination / Q000187:drug effects* / Q000502:physiology*
chemical_list: D010666:Phenylpropionates; C040656:loxoprofen; D005998:Glycine