title: Ischemia modified albumin increase indicating cardiac damage after experimental subarachnoid hemorrhage.
abstract: BACKGROUND
  Cardiac complications are often developed after subarachnoid hemorrhage (SAH) and may cause sudden death of the patient. There are reports in the literature addressing ischemia modified albumin (IMA) as an early and useful marker in the diagnosis of ischemic heart events. The aim of this study is to evaluate serum IMA by using the albumin cobalt binding (ACB) test in the first, second, and seventh days of experimental SAH in rats.Twenty-eight Wistar albino rats were divided into four groups each consisting of seven animals. These were classified as control group, 1st, 2nd and 7th day SAH groups. SAH was done by transclival basilar artery puncture. Blood samples were collected under anesthesia from the left ventricles of the heart using the cardiac puncture method for IMA measurement. Histopathological examinations were performed on the heart and lung tissues. Albumin with by colorimetric, creatine kinase (CK), aspartate aminotransferase (AST), lactate dehydrogenase (LDH) were determined on an automatic analyser using the enzymatic method. IMA using by ACB test was detected with spectrophotometer.
  
  
  RESULTS
  Serum IMA (p = 0.044) in seventh day of SAH were higher compared to the control group. Total injury scores of heart and lung tissue, also myocytolysis at day 7 were significantly higher than control group (p = 0.001, p = 0.001, p = 0.001), day 1 (p = 0.001, p = 0.001, p = 0.001) and day 2 (p = 0.001, p = 0.007, p = 0.001). A positive correlation between IMA - myocytolysis (r = 0.48, p = 0.008), and between IMA - heart tissue total injury score (r = 0.41, p = 0.029) was found.
  
  
  CONCLUSIONS
  The results revealed that increased serum IMA may be related to myocardial stress after SAH.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Bülent Ecevit University (Formerly, Zonguldak Karaelmas University), 67630, Esenköy, Kozlu, Zonguldak, Turkey. serefdenacikgoz@yahoo.com.
authors.forename: Şerefden
authors.initials: Ş
authors.lastname: Açıkgöz
authors.forename: Nurullah
authors.initials: N
authors.lastname: Edebali
authors.forename: Figen
authors.initials: F
authors.lastname: Barut
authors.forename: Murat
authors.initials: M
authors.lastname: Can
authors.forename: İshak Özel
authors.initials: İÖ
authors.lastname: Tekin
authors.forename: Çağatay
authors.initials: Ç
authors.lastname: Büyükuysal
authors.forename: Bektaş
authors.initials: B
authors.lastname: Açıkgöz
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D008297:Male; D017202:Myocardial Ischemia / Q000097:blood* / Q000175:diagnosis / Q000209:etiology*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012709:Serum Albumin; D000075462:Serum Albumin, Human; D013345:Subarachnoid Hemorrhage / Q000097:blood* / Q000150:complications* / Q000175:diagnosis
chemical_list: D015415:Biomarkers; D012709:Serum Albumin; C561522:ischemia-modified albumin; D000075462:Serum Albumin, Human