title: Generation and characterization of chicken-sourced single-chain variable fragments (scFvs) against porcine interferon-gamma (pIFN-γ).
abstract: Development of chicken-sourced antibodies offers an alternative strategy for the development of highly specific antibodies against mammalian proteins with conserved epitopes due to the phylogenetic distance between avian and mammalian species. In this study, the single-chain variable fragments (scFvs) against porcine interferon-gamma was screened and characterized from a hyperimmunized chicken phage display library. The expressed soluble scFvs exhibited highly specific recognition of porcine interferon-gamma in ELISA, Western blot, and immunofluorescence staining assays. Results of the current study indicate that it is possible to develop scFv IgY antibodies to a mammalian interferon by using Biopanning technology. Furthermore, it also confirms that monoclonal avian IgY antibody technique could be applied as a promising tool to produce immunoglobulin molecules with high specificity and affinity towards conserved mammalian epitopes or antigens.
authors.affiliation: a Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agriculture Science , Harbin , Heilongjiang , China.
authors.forename: Hong-Xiu
authors.initials: HX
authors.lastname: Chen
authors.forename: Fan
authors.initials: F
authors.lastname: He
authors.forename: Yuan
authors.initials: Y
authors.lastname: Sun
authors.forename: Yuzi
authors.initials: Y
authors.lastname: Luo
authors.forename: Hua-Ji
authors.initials: HJ
authors.lastname: Qiu
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Zhang
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Sutton
mesh_terms: D000818:Animals; D000937:Antigen-Antibody Reactions; D002645:Chickens; D007128:Immunoglobulin Fragments / Q000276:immunology*; D007371:Interferon-gamma / Q000276:immunology*; D057127:Single-Chain Antibodies / Q000276:immunology*; D013552:Swine
chemical_list: D007128:Immunoglobulin Fragments; D057127:Single-Chain Antibodies; D007371:Interferon-gamma
keywords: chicken monoclonal IgY antibody (mIgY); mammalian conserved proteins; phage display; porcine interferon-gamma (pIFN-γ); single-chain variable fragments (scFv)