title: Spray drying as a strategy for biosurfactant recovery, concentration and storage.
abstract: The objective of this study was to analyze the use of Spray Drying for concentration and preservation of biosurfactants produced by Bacillus subtilis LBBMA RI4914 isolated from a heavy oil reservoir. Kaolinite and maltodextrin 10DE or 20DE were tested as drying adjuvants. Surface activity of the biosurfactant was analyzed by preparing dilution x surface activity curves of crude biosurfactant, crude biosurfactant plus adjuvants and of the dried products, after their reconstitution in water. The shelf life of the dried products was also evaluated. Spray drying was effective in the recovery and concentration of biosurfactant, while keeping its surface activity. Drying adjuvants were required to obtain a solid product with the desired characteristics. These compounds did not interfere with tensoactive properties of the biosurfactant molecules. The dehydrated product maintained its surfactant properties during storage at room temperature during the evaluation period (120 days), with no detectable loss of activity.
authors.affiliation: Departamento de Microbiologia, Universidade Federal de Viçosa. ECS, sala 141. Campus da UFV, Centro, Viçosa, MG 36570000 Brazil.
authors.forename: Gisely S
authors.initials: GS
authors.lastname: Barcelos
authors.affiliation: Departamento de Microbiologia, Universidade Federal de Viçosa. ECS, sala 141. Campus da UFV, Centro, Viçosa, MG 36570000 Brazil.
authors.forename: Lívia C
authors.initials: LC
authors.lastname: Dias
authors.affiliation: Departamento de Microbiologia, Universidade Federal de Viçosa. ECS, sala 141. Campus da UFV, Centro, Viçosa, MG 36570000 Brazil.
authors.forename: Péricles L
authors.initials: PL
authors.lastname: Fernandes
authors.affiliation: Departamento de Microbiologia, Universidade Federal de Viçosa. ECS, sala 141. Campus da UFV, Centro, Viçosa, MG 36570000 Brazil.
authors.forename: Rita de Cássi R
authors.initials: Rde C
authors.lastname: Fernandes
authors.affiliation: Departamento de Microbiologia, Universidade Federal de Viçosa. ECS, sala 141. Campus da UFV, Centro, Viçosa, MG 36570000 Brazil.
authors.forename: Arnaldo C
authors.initials: AC
authors.lastname: Borges
authors.affiliation: Departamento de Microbiologia, Universidade Federal de Viçosa. ECS, sala 141. Campus da UFV, Centro, Viçosa, MG 36570000 Brazil.
authors.forename: Karlos Hm
authors.initials: KH
authors.lastname: Kalks
authors.affiliation: Departamento de Microbiologia, Universidade Federal de Viçosa. ECS, sala 141. Campus da UFV, Centro, Viçosa, MG 36570000 Brazil.
authors.forename: Marcos R
authors.initials: MR
authors.lastname: Tótola
keywords: Biosurfactant; Clay; Environmental biotechnology; Maltodextrin; Spray drying; Surfactin