title: Transcriptional regulation of miR-146b by C/EBPβ LAP2 in esophageal cancer cells.
abstract: Recent clinical study indicated that up-regulation of miR-146b was associated with poor overall survival of patients in esophageal squamous cell carcinoma. However, the underlying mechanism of miR-146b dysregulation remains to be explored. Here we report that miR-146b promotes cell proliferation and inhibits cell apoptosis in esophageal cancer cell lines. Mechanismly, two C/EBPβ binding motifs are located in the miR-146b promoter conserved region. Among the three isoforms of C/EBPβ, C/EBPβ LAP2 positively regulated miR-146b expression and increases miR-146b levels in a dose-dependent manner through transcription activation of miR-146b gene. Together, these results suggest a miR-146b regulatory mechanism involving C/EBPβ, which may contribute to the up-regulation of miR-146b in esophageal squamous cell carcinoma.
authors.affiliation: Department of Medical Genetics, Third Military Medical University, Chongqing, People's Republic of China.
authors.forename: Junxia
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Pathophysiology and High Altitude Physiology, Third Military Medical University, Chongqing, People's Republic of China.
authors.forename: Fabo
authors.initials: F
authors.lastname: Shan
authors.affiliation: Department of Thoracic and Cardiac Surgery, Southwest Hospital, Third Military Medical University, Chongqing, People's Republic of China.
authors.forename: Gang
authors.initials: G
authors.lastname: Xiong
authors.affiliation: Institute of Bioinformatics and Biosignal Transduction, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Ju-Ming
authors.initials: JM
authors.lastname: Wang
authors.affiliation: Institute of Bioinformatics and Biosignal Transduction, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Wen-Lin
authors.initials: WL
authors.lastname: Wang
authors.affiliation: Molecular Biology Center, State Key Laboratory of Trauma, Burn, and Combined Injury, Research Institute of Surgery and Daping Hospital, Third Military Medical University, Chongqing, People's Republic of China. Electronic address: buyi.chu@gmail.com.
authors.forename: Xueqing
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Medical Genetics, Third Military Medical University, Chongqing, People's Republic of China. Electronic address: baiyungene@gmail.com.
authors.forename: Yun
authors.initials: Y
authors.lastname: Bai
mesh_terms: D017209:Apoptosis; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D017124:Conserved Sequence; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D020033:Protein Isoforms / Q000378:metabolism; D015533:Transcriptional Activation; D015854:Up-Regulation
chemical_list: D022782:CCAAT-Enhancer-Binding Protein-beta; C542542:MIRN146 microRNA, human; D035683:MicroRNAs; D020033:Protein Isoforms
keywords: C/EBPβ; ESCC; miR-146b; microRNA