title: Postpartal subclinical endometritis alters transcriptome profiles in liver and adipose tissue of dairy cows.
abstract: Transcriptome alterations in liver and adipose tissue of cows with subclinical endometritis (SCE) at 29 d postpartum were evaluated. Bioinformatics analysis was performed using the Dynamic Impact Approach by means of KEGG and DAVID databases. Milk production, blood metabolites (non-esterified fatty acids, magnesium), and disease biomarkers (albumin, aspartate aminotransferase) did not differ greatly between healthy and SCE cows. In liver tissue of cows with SCE, alterations in gene expression revealed an activation of complement and coagulation cascade, steroid hormone biosynthesis, apoptosis, inflammation, oxidative stress, MAPK signaling, and the formation of fibrinogen complex. Bioinformatics analysis also revealed an inhibition of vitamin B3 and B6 metabolism with SCE. In adipose, the most activated pathways by SCE were nicotinate and nicotinamide metabolism, long-chain fatty acid transport, oxidative phosphorylation, inflammation, T cell and B cell receptor signaling, and mTOR signaling. Results indicate that SCE in dairy cattle during early lactation induces molecular alterations in liver and adipose tissue indicative of immune activation and cellular stress.
authors.affiliation: Department of Animal Sciences, University of Illinois, Urbana, Illinois, USA.
authors.forename: Haji
authors.initials: H
authors.lastname: Akbar
authors.affiliation: Department of Animal Sciences, University of Illinois, Urbana, Illinois, USA.
authors.forename: Felipe C
authors.initials: FC
authors.lastname: Cardoso
authors.affiliation: DairyNZ Limited, Hamilton, New Zealand.
authors.forename: Susanne
authors.initials: S
authors.lastname: Meier
authors.affiliation: DairyNZ Limited, Hamilton, New Zealand.
authors.forename: Christopher
authors.initials: C
authors.lastname: Burke
authors.affiliation: Cognosco, Animal Health Centre, Morrinsville, New Zealand.
authors.forename: Scott
authors.initials: S
authors.lastname: McDougall
authors.affiliation: Liggins Institute, University of Auckland, Auckland, New Zealand. ; University of Queensland Centre for Clinical Research, Brisbane, St. Lucia, Queensland, Australia.
authors.forename: Murray
authors.initials: M
authors.lastname: Mitchell
authors.affiliation: DairyNZ Limited, Hamilton, New Zealand.
authors.forename: Caroline
authors.initials: C
authors.lastname: Walker
authors.affiliation: Department of Animal Sciences, University of Illinois, Urbana, Illinois, USA.
authors.forename: Sandra L
authors.initials: SL
authors.lastname: Rodriguez-Zas
authors.affiliation: Department of Animal Sciences, University of Illinois, Urbana, Illinois, USA.
authors.forename: Robin E
authors.initials: RE
authors.lastname: Everts
authors.affiliation: Department of Animal Sciences, University of Illinois, Urbana, Illinois, USA.
authors.forename: Harris A
authors.initials: HA
authors.lastname: Lewin
authors.affiliation: DairyNZ Limited, Hamilton, New Zealand.
authors.forename: John R
authors.initials: JR
authors.lastname: Roche
authors.affiliation: Department of Animal Sciences, University of Illinois, Urbana, Illinois, USA. ; Division of Nutritional Sciences, University of Illinois, Urbana, Illinois, USA.
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Loor
keywords: adipose; cow genomics; liver; uterine infection