title: Association of mannose-binding lectin 2 (mbl2) gene heterogeneity and its serum concentration with osteoporosis in postmenopausal women.
abstract: The aim of the study was to detect prevalence of MBL2 exon 1 (codons 52, 54 and 57) genetic polymorphism in postmenopausal women in Bosnia and Herzegovina and its possible role as genetic risk factor for susceptibility to occurrence of osteoporosis in this study group. Also, we investigated association between MBL serum concentrations and osteoporosis in postmenopausal women. Genetic codons' variations were determined by PCR-RFLP and MBL in serum was measured by ELISA method in 75 postmenopausal women (37 with osteoporosis and 38 apparently healthy, non-osteoporotic women serving as a control). Serum MBL levels were not significantly different between osteoporosis and control group (492 (37-565.1) and 522.6 (477-559.4) ng/mL respectively, p=0.206). Genotype frequencies were not significantly different (p=0.997) between the studied groups of postmenopausal women. Genotype frequencies A/A, A/0 and 0/0 in osteoporosis group were 0.576; 0.405; 0.018 and in control group 0.562; 0.412; 0.026, respectively. Frequencies of A and 0 allele were 0.78 and 0.22 in osteoporosis and 0.77 and 0.23 in control group. The results do not suggest association of functional polymorphism of MBL2 gene and MBL serum concentration with osteoporosis in postmenopausal females.
authors.affiliation: Laboratory for Molecular Medicine, Center for Genetics, Faculty of Medicine, University of Sarajevo, Čekaluša 90, 71 000 Sarajevo, Bosnia and Herzegovina.
authors.forename: Emina
authors.initials: E
authors.lastname: Kiseljaković
authors.affiliation: Laboratory for Molecular Medicine, Center for Genetics, Faculty of Medicine, University of Sarajevo, Čekaluša 90, 71 000 Sarajevo, Bosnia and Herzegovina.
authors.forename: Sabaheta
authors.initials: S
authors.lastname: Hasić
authors.affiliation: Laboratory for Molecular Medicine, Center for Genetics, Faculty of Medicine, University of Sarajevo, Čekaluša 90, 71 000 Sarajevo, Bosnia and Herzegovina.
authors.forename: Amina
authors.initials: A
authors.lastname: Valjevac
authors.affiliation: Laboratory for Molecular Medicine, Center for Genetics, Faculty of Medicine, University of Sarajevo, Čekaluša 90, 71 000 Sarajevo, Bosnia and Herzegovina.
authors.forename: Mirela
authors.initials: M
authors.lastname: Mačkić-Đurović
authors.affiliation: Laboratory for Molecular Medicine, Center for Genetics, Faculty of Medicine, University of Sarajevo, Čekaluša 90, 71 000 Sarajevo, Bosnia and Herzegovina.
authors.forename: Radivoj
authors.initials: R
authors.lastname: Jadrić
authors.affiliation: Laboratory for Molecular Medicine, Center for Genetics, Faculty of Medicine, University of Sarajevo, Čekaluša 90, 71 000 Sarajevo, Bosnia and Herzegovina.
authors.forename: Bakir
authors.initials: B
authors.lastname: Mehić
authors.affiliation: Institute of Nuclear Medicine, Clinical Center University of Sarajevo, Bolnička 25, 71 000 Sarajevo, Bosnia and Herzegovina.
authors.forename: Elma
authors.initials: E
authors.lastname: Kučukalić-Selimović
authors.affiliation: Laboratory for Molecular Medicine, Center for Genetics, Faculty of Medicine, University of Sarajevo, Čekaluša 90, 71 000 Sarajevo, Bosnia and Herzegovina.
authors.forename: Slavka
authors.initials: S
authors.lastname: Ibrulj
mesh_terms: D000368:Aged; D000483:Alleles; D015519:Bone Density; D003062:Codon; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D037601:Mannose-Binding Lectin / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D015663:Osteoporosis, Postmenopausal / Q000235:genetics*; D011110:Polymorphism, Genetic; D017698:Postmenopause
chemical_list: D003062:Codon; C445619:MBL2 protein, human; D037601:Mannose-Binding Lectin