title: Cellular sources of tenascin-C in canine mammary carcinomas.
abstract: Tenascin-C (Tn-C) is an extracellular matrix glycoprotein implicated in the progression of several human cancers. In canine mammary carcinomas, accumulation of Tn-C has been recognized in 3 different areas: regions of proliferating myoepithelial cells in complex carcinoma, basement membrane zone in low-grade simple carcinoma, and reactive stroma in high-grade simple carcinoma. To identify the Tn-C synthesizing cells in these areas, we utilized double-labeling immunohistochemistry, branched DNA in situ hybridization, and in situ hybridization-immunohistochemistry double-labeling techniques. In complex carcinomas, Tn-C was generated by proliferating myoepithelial cells. Tn-C in low-grade simple carcinomas was also derived from myoepithelial cells existing as a basal monolayer. However, stromal Tn-C in high-grade carcinomas was mainly synthesized by fibroblasts/myofibroblasts, similar to human breast cancer. Thus, the origin of Tn-C in canine mammary carcinomas differs between low- and high-grade malignancies. The role of myoepithelial cell-generated Tn-C is not yet understood.
authors.affiliation: Department of Veterinary Pathology, Nippon Veterinary and Life Science University, Tokyo, Japan Departments of Pathology and Integrative Oncological Pathology, Nippon Medical School, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yoshimura
authors.affiliation: Department of Veterinary Pathology, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Michishita
authors.affiliation: Department of Veterinary Pathology, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ohkusu-Tsukada
authors.affiliation: Departments of Pathology and Integrative Oncological Pathology, Nippon Medical School, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuda
authors.affiliation: Departments of Pathology and Integrative Oncological Pathology, Nippon Medical School, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ishiwata
authors.affiliation: Departments of Pathology and Integrative Oncological Pathology, Nippon Medical School, Tokyo, Japan.
authors.forename: Z
authors.initials: Z
authors.lastname: Naito
authors.affiliation: Department of Veterinary Pathology, Nippon Veterinary and Life Science University, Tokyo, Japan kimimasa@nvlu.ac.jp.
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D018450:Disease Progression; D004283:Dog Diseases / Q000378:metabolism / Q000473:pathology*; D004285:Dogs; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology; D017403:In Situ Hybridization / Q000662:veterinary; D015674:Mammary Neoplasms, Animal / Q000378:metabolism / Q000473:pathology*; D058628:Myofibroblasts / Q000378:metabolism / Q000473:pathology; D019063:Tenascin / Q000378:metabolism
chemical_list: D019063:Tenascin
keywords: dogs; immunohistochemistry; in situ hybridization; mammary carcinoma; mammary tumor; myoepithelial cell; myofibroblast; tenascin-C