title: In vitro and in vivo antitumor activity of Bulbus Fritillariae Cirrhosae and preliminary investigation of its mechanism.
abstract: Bulbus Fritillariae Cirrhosae (BFC) is widely used in China both for food and folk medicine because of its powerful biological activities. Firstly, this study was designed to examine the antiproliferative activities of the different fractions from BFC in vitro by MTT assay. The results showed that chloroform extracts (CE) and the purified total alkaloids of BFC (TAF) exhibited stronger antiproliferative activity than the other fractions. We further determined the total alkaloids and 3 main alkaloids monomers content of CE and TAF by UV and HPLC-ELSD methods, respectively. Moreover, we assessed the antitumor activity of TAF in vivo and made preliminary investigation of its antitumor mechanism by histological and immunohistochemical staining technique. These results demonstrate that TAF showed significant antitumor activity and low toxicity in vivo. Meanwhile, TAF significantly inhibited tumor angiogenesis and induced apoptosis by improvement of expression level of caspase-3. These results suggest that alkaloids of BFC could hold a good potential for use as an antitumor drug.
authors.affiliation: a Department of Pharmacognosy, West China College of Pharmacy , Sichuan University , Chengdu , PR China.
authors.forename: Dong-Dong
authors.initials: DD
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Feng
authors.forename: Zu
authors.initials: Z
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
authors.forename: Chao-Yang
authors.initials: CY
authors.lastname: Zhang
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Wang
authors.forename: Zi-Yu
authors.initials: ZY
authors.lastname: Liu
mesh_terms: D000470:Alkaloids / Q000032:analysis / Q000494:pharmacology*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor / Q000187:drug effects; D002595:Cevanes / Q000032:analysis / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry*; D006801:Humans; D019668:Liliaceae / Q000737:chemistry*; D008297:Male; D008813:Mice, Inbred ICR; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D013256:Steroids / Q000032:analysis / Q000737:chemistry
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D002595:Cevanes; D004365:Drugs, Chinese Herbal; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D013256:Steroids; C000597836:chuanbeinone; C052510:peimisine; C505409:Casp3 protein, mouse; D053148:Caspase 3; C047331:peiminine