title: Evaluation of silicon nitride as a substrate for culture of PC12 cells: an interfacial model for functional studies in neurons.
abstract: Silicon nitride is a biocompatible material that is currently used as an interfacial surface between cells and large-scale integration devices incorporating ion-sensitive field-effect transistor technology. Here, we investigated whether a poly-L-lysine coated silicon nitride surface is suitable for the culture of PC12 cells, which are widely used as a model for neural differentiation, and we characterized their interaction based on cell behavior when seeded on the tested material. The coated surface was first examined in terms of wettability and topography using contact angle measurements and atomic force microscopy and then, conditioned silicon nitride surface was used as the substrate for the study of PC12 cell culture properties. We found that coating silicon nitride with poly-L-lysine increased surface hydrophilicity and that exposing this coated surface to an extracellular aqueous environment gradually decreased its roughness. When PC12 cells were cultured on a coated silicon nitride surface, adhesion and spreading were facilitated, and the cells showed enhanced morphological differentiation compared to those cultured on a plastic culture dish. A bromodeoxyuridine assay demonstrated that, on the coated silicon nitride surface, higher proportions of cells left the cell cycle, remained in a quiescent state and had longer survival times. Therefore, our study of the interaction of the silicon nitride surface with PC12 cells provides important information for the production of devices that need to have optimal cell culture-supporting properties in order to be used in the study of neuronal functions.
authors.affiliation: Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology, Toyohashi, Japan ; Core Research for Evolutional Science and Technology Program, Japan Science and Technology Agency, Tokyo, Japan.
authors.forename: Johan Jaime
authors.initials: JJ
authors.lastname: Medina Benavente
authors.affiliation: Core Research for Evolutional Science and Technology Program, Japan Science and Technology Agency, Tokyo, Japan ; Faculty of Health Promotional Sciences, Hamamatsu University, Hamamatsu, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Mogami
authors.affiliation: Core Research for Evolutional Science and Technology Program, Japan Science and Technology Agency, Tokyo, Japan ; Electronics-Inspired Interdisciplinary Research Institute, Toyohashi University of Technology, Toyohashi, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Sakurai
authors.affiliation: Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology, Toyohashi, Japan ; Core Research for Evolutional Science and Technology Program, Japan Science and Technology Agency, Tokyo, Japan.
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Sawada
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D022001:Focal Adhesions; D020932:Nerve Growth Factor / Q000494:pharmacology; D009474:Neurons; D016716:PC12 Cells; D011107:Polylysine / Q000737:chemistry; D051381:Rats; D017655:Silicon Compounds / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D017655:Silicon Compounds; D011107:Polylysine; D020932:Nerve Growth Factor; C032734:silicon nitride