title: Global identification of caspase substrates using PROTOMAP (protein topography and migration analysis platform).
abstract: Delineation of the natural substrate scope of proteases is important for understanding the functions of proteolytic pathways in physiology and disease. Herein we describe the protocol for PROTOMAP, a technique that combines SDS-PAGE with tandem mass spectrometry to globally identify shifts in protein migration indicative of proteolytic processing. When applied to cells undergoing apoptosis, this unbiased global method provides a snapshot of the topography and magnitude of proteolytic events associated with programmed cell death.
authors.affiliation: Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA, USA.
authors.forename: Melissa M
authors.initials: MM
authors.lastname: Dix
authors.forename: Gabriel M
authors.initials: GM
authors.lastname: Simon
authors.forename: Benjamin F
authors.initials: BF
authors.lastname: Cravatt
mesh_terms: D017209:Apoptosis / Q000235:genetics; D020169:Caspases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D040901:Proteomics; D013379:Substrate Specificity; D053719:Tandem Mass Spectrometry
chemical_list: D020169:Caspases