title: Origin of porcine circovirus type 2 (PCV2) from swine affected by PCV2-associated diseases in Croatia.
abstract: Porcine circovirus type 2 (PCV2) causes some of the most significant economic losses in pig production. Several multisystemic syndromes have been attributed to PCV2 infection, which are known as PCV2-associated diseases (PCVDs). This study investigated the origin and evolution of PCV2 sequences in domestic pigs and wild boars affected by PCVDs in Croatia. Viral sequences were recovered from three wild boars diagnosed with PCV2-systemic disease (PCV2-SD), 63 fetuses positive for PCV2 DNA as determined by PCR, 14 domestic pigs affected with PCV2-SD (displaying severe interstitial nephritis) and five domestic pigs with proliferative and necrotising pneumonia. Seventeen complete PCV2 genomes were recovered. Phylogenetic and evolutionary analyses based on median-joining phylogenetic networks, amino acid alignments and principal coordinate analysis were performed using complete genomes, as well as complete and partial ORF sequences for ORF1 and ORF2. Two of the 17 PCV2 sequences belonged to PCV2a, 14 to PCV2b and one was unclustered. PCV2b was the predominant genotype in Croatia and has been linked to international trade as a route of introduction. Correlation between particular viral strains with PCVDs is lacking.
authors.affiliation: Department of Pathology, Croatian Veterinary Institute, Zagreb 10000, Croatia.
authors.forename: D
authors.initials: D
authors.lastname: Novosel
authors.forename: T
authors.initials: T
authors.lastname: Tuboly
authors.forename: A
authors.initials: A
authors.lastname: Csagola
authors.forename: M
authors.initials: M
authors.lastname: Lorincz
authors.forename: V
authors.initials: V
authors.lastname: Cubric-Curik
authors.forename: A
authors.initials: A
authors.lastname: Jungic
authors.forename: I
authors.initials: I
authors.lastname: Curik
authors.forename: J
authors.initials: J
authors.lastname: Segalés
authors.forename: M
authors.initials: M
authors.lastname: Cortey
authors.forename: Z
authors.initials: Z
authors.lastname: Lipej
mesh_terms: D000818:Animals; D018173:Circoviridae Infections / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000235:genetics*; D017523:Croatia; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000821:virology*
chemical_list: D004279:DNA, Viral
keywords: Phylogenetic analysis; Porcine circovirus type 2; Porcine circovirus-associated disease