title: Construction and direct electrochemistry of orientation controlled laccase electrode.
abstract: A laccase has multiple redox centres. Chemisorption of laccases on a gold electrode through a polypeptide tag introduced at the protein surface provides an isotropic orientation of laccases on the Au surface, which allows the orientation dependent study of the direct electrochemistry of laccase. In this paper, using genetic engineering technology, two forms of recombinant laccase which has Cys-6×His tag at the N or C terminus were generated. Via the Au-S linkage, the recombinant laccase was assembled orientationally on gold electrode. A direct electron transfer and a bioelectrocatalytic activity toward oxygen reduction were observed on the two orientation controlled laccase electrodes, but their electrochemical behaviors were found to be quite different. The orientation of laccase on the gold electrode affects both the electron transfer pathway and the electron transfer efficiency of O2 reduction. The present study is helpful not only to the in-depth understanding of the direct electrochemistry of laccase, but also to the development of laccase-based biofuel cells.
authors.affiliation: Key Laboratory of Colloid & Interface Chemistry of the Education Ministry of China, Shandong University, Jinan 250100, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Microbial Technology of China, Shandong University, Jinan 250100, PR China.
authors.forename: Jiwei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Colloid & Interface Chemistry of the Education Ministry of China, Shandong University, Jinan 250100, PR China; State Key Laboratory of Microbial Technology of China, Shandong University, Jinan 250100, PR China. Electronic address: xrhuang@sdu.edu.cn.
authors.forename: Xirong
authors.initials: X
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Microbial Technology of China, Shandong University, Jinan 250100, PR China. Electronic address: wangtianhong@sdu.edu.cn.
authors.forename: Tianhong
authors.initials: T
authors.lastname: Wang
mesh_terms: D001674:Bioelectric Energy Sources; D015374:Biosensing Techniques; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005800:Genes, Fungal; D006046:Gold; D042845:Laccase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D011487:Protein Conformation; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D055454:Trametes / Q000201:enzymology / Q000235:genetics
chemical_list: D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D011994:Recombinant Proteins; D006046:Gold; D042845:Laccase
keywords: Direct electron transfer; Electron transfer pathway; Gold electrode; Oriented immobilization; Recombinant laccase