title: Vascular injury post stent implantation: different gene expression modulation in human umbilical vein endothelial cells (HUVECs) model.
abstract: To explore whether stent procedure may influence transcriptional response of endothelium, we applied different physical (flow changes) and/or mechanical (stent application) stimuli to human endothelial cells in a laminar flow bioreactor (LFB) system. Gene expression analysis was then evaluated in each experimental condition. Human umbilical vein endothelial cells (HUVECs) were submitted to low and physiological (1 and 10 dyne/cm(2)) shear stress in absence (AS) or presence (PS) of stent positioning in a LFB system for 24 h. Different expressed genes, coming from Affymetrix results, were identified based on one-way ANOVA analysis with p values <0.01 and a fold changed >3 in modulus. Low shear stress was compared with physiological one in AS and PS conditions. Two major groups include 32 probes commonly expressed in both 1AS versus 10AS and 1PS versus 10PS comparison, and 115 probes consisting of 83 in addition to the previous 32, expressed only in 1PS versus 10PS comparison. Genes related to cytoskeleton, extracellular matrix, and cholesterol transport/metabolism are differently regulated in 1PS versus 10PS condition. Inflammatory and apoptotic mediators seems to be, instead, closely modulated by changes in flow (1 versus 10), independently of stent application. Low shear stress together with stent procedure are the experimental conditions that mainly modulate the highest number of genes in our human endothelial model. Those genes belong to pathways specifically involved in the endothelial dysfunction.
authors.affiliation: CNR Institute of Clinical Physiology, Milan and Pisa, Italy.
authors.forename: Jonica
authors.initials: J
authors.lastname: Campolo
authors.affiliation: CNR Institute of Clinical Physiology, Milan and Pisa, Italy.
authors.forename: Federico
authors.initials: F
authors.lastname: Vozzi
authors.affiliation: Department of Laboratory Medicine, Medical Genetics, Niguarda Ca' Granda Hospital Milan, Italy.
authors.forename: Silvana
authors.initials: S
authors.lastname: Penco
authors.affiliation: CNR Institute of Clinical Physiology, Milan and Pisa, Italy.
authors.forename: Lorena
authors.initials: L
authors.lastname: Cozzi
authors.affiliation: CNR Institute of Clinical Physiology, Milan and Pisa, Italy.
authors.forename: Raffaele
authors.initials: R
authors.lastname: Caruso
authors.affiliation: CNR Institute of Clinical Physiology, Milan and Pisa, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Domenici
authors.affiliation: Interdepartmental Research Centre "E. Piaggio", University of Pisa, Pisa, Italy.
authors.forename: Arti
authors.initials: A
authors.lastname: Ahluwalia
authors.affiliation: CNR Institute of Clinical Physiology, Milan and Pisa, Italy.
authors.forename: Michela
authors.initials: M
authors.lastname: Rial
authors.affiliation: CNR Institute of Clinical Physiology, Milan and Pisa, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Marraccini
authors.affiliation: CNR Institute of Clinical Physiology, Milan and Pisa, Italy.
authors.forename: Oberdan
authors.initials: O
authors.lastname: Parodi
mesh_terms: D002470:Cell Survival; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D015607:Stents / Q000009:adverse effects*; D013314:Stress, Mechanical; D014158:Transcription, Genetic; D059467:Transcriptome; D057772:Vascular System Injuries / Q000209:etiology* / Q000235:genetics* / Q000473:pathology