title: Co-modulated behavior and effects of differentially expressed miRNA in colorectal cancer.
abstract: BACKGROUND
  MicroRNAs (miRNAs) are short noncoding RNAs (approximately 22 nucleotides in length) that play important roles in colorectal cancer (CRC) progression through silencing gene expression. Numerous dysregulated miRNAs simultaneously participate in the process of colon cancer development. However, the detailed mechanisms and biological functions of co-expressed miRNA in colorectal carcinogenesis have yet to be fully elucidated.
  
  
  RESULTS
  The objective of this study was to identify the dysfunctional miRNAs and their target mRNAs using a wet-lab experimental and dry-lab bioinformatics approach. The differentially expressed miRNA candidates were identified from 2 miRNA profiles, and were confirmed in CRC clinical samples using reported target genes of dysfunctional miRNAs to perform functional pathway enrichment analysis. Potential target gene candidates were predicted by an in silico search, and their expression levels between normal and colorectal tumor tissues were further analyzed using real-time polymerase chain reaction (RT-PCR).
  
  
  CONCLUSIONS
  Fifteen dysfunctional miRNAs were engaged in metastasis-associated pathways through comodulating 7 target genes, which were identified by using a multi-step approach. The roles of these candidate genes are worth further exploration in the progression of colon cancer, and could potentially be targets in future therapy.
authors.forename: Wei-Shone
authors.initials: WS
authors.lastname: Chen
authors.forename: Ting-Wen
authors.initials: TW
authors.lastname: Chen
authors.forename: Tzu-Hsien
authors.initials: TH
authors.lastname: Yang
authors.forename: Ling-Yueh
authors.initials: LY
authors.lastname: Hu
authors.forename: Hung-Wei
authors.initials: HW
authors.lastname: Pan
authors.forename: Chung-Man
authors.initials: CM
authors.lastname: Leung
authors.forename: Sung-Chou
authors.initials: SC
authors.lastname: Li
authors.forename: Meng-Ru
authors.initials: MR
authors.lastname: Ho
authors.forename: Chih-Wen
authors.initials: CW
authors.lastname: Shu
authors.forename: Pei-Feng
authors.initials: PF
authors.lastname: Liu
authors.forename: Shou-Yu
authors.initials: SY
authors.lastname: Yu
authors.forename: Ya-Ting
authors.initials: YT
authors.lastname: Tu
authors.forename: Wen-Chang
authors.initials: WC
authors.lastname: Lin
authors.forename: Tony T
authors.initials: TT
authors.lastname: Wu
authors.forename: Kuo-Wang
authors.initials: KW
authors.lastname: Tsai
mesh_terms: D002448:Cell Adhesion; D002453:Cell Cycle; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D019295:Computational Biology; D058750:Epithelial-Mesenchymal Transition; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009362:Neoplasm Metastasis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger