title: Comprehensive portrait of cholesterol containing oxidized membrane.
abstract: Biological membranes are under significant oxidative stress caused by reactive oxygen species mostly originating during cellular respiration. Double bonds of the unsaturated lipids are most prone to oxidation, which might lead to shortening of the oxidized chain and inserting of terminal either aldehyde or carboxylic group. Structural rearrangement of oxidized lipids, addressed already, is mainly associated with looping back of the hydrophilic terminal group. This contribution utilizing dual-focus fluorescence correlation spectroscopy and electron paramagnetic resonance as well as atomistic molecular dynamics simulations focuses on the overall changes of the membrane structural and dynamical properties once it becomes oxidized. Particularly, attention is paid to cholesterol rearrangement in the oxidized membrane revealing its preferable interaction with carbonyls of the oxidized chains. In this view cholesterol seems to have a tendency to repair, rather than condense, the bilayer.
authors.affiliation: J. Heyrovský Institute of Physical Chemistry of the Academy of Sciences of the Czech Republic, v. v. i., Dolejškova 2155/3, 182 23 Prague 8, Czech Republic. Electronic address: martin.stefl@jh-inst.cas.cz.
authors.forename: Martin
authors.initials: M
authors.lastname: Stefl
authors.affiliation: J. Heyrovský Institute of Physical Chemistry of the Academy of Sciences of the Czech Republic, v. v. i., Dolejškova 2155/3, 182 23 Prague 8, Czech Republic. Electronic address: radek.sachl@jh-inst.cs.cz.
authors.forename: Radek
authors.initials: R
authors.lastname: Sachl
authors.affiliation: J. Heyrovský Institute of Physical Chemistry of the Academy of Sciences of the Czech Republic, v. v. i., Dolejškova 2155/3, 182 23 Prague 8, Czech Republic. Electronic address: agnieszka.olzynska@jh-inst.cas.cz.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Olżyńska
authors.affiliation: J. Heyrovský Institute of Physical Chemistry of the Academy of Sciences of the Czech Republic, v. v. i., Dolejškova 2155/3, 182 23 Prague 8, Czech Republic. Electronic address: mariana.amaro@jh-inst.cas.cz.
authors.forename: Mariana
authors.initials: M
authors.lastname: Amaro
authors.affiliation: Institute of Physics, Academy of Sciences of the Czech Republic, v. v. i., Na Slovance 1999/2, 182 21 Prague 8, Czech Republic. Electronic address: savchenko@fzu.cz.
authors.forename: Dariya
authors.initials: D
authors.lastname: Savchenko
authors.affiliation: Institute of Physics, Academy of Sciences of the Czech Republic, v. v. i., Na Slovance 1999/2, 182 21 Prague 8, Czech Republic. Electronic address: dejneka@fzu.cz.
authors.forename: Alexander
authors.initials: A
authors.lastname: Deyneka
authors.affiliation: Institute of Biochemistry, University of Technology, Graz, Petersgasse 12/II, A-8010 Graz, Austria. Electronic address: albin.hermetter@tugraz.at.
authors.forename: Albin
authors.initials: A
authors.lastname: Hermetter
authors.affiliation: J. Heyrovský Institute of Physical Chemistry of the Academy of Sciences of the Czech Republic, v. v. i., Dolejškova 2155/3, 182 23 Prague 8, Czech Republic. Electronic address: lukasz.cwiklik@jh-inst.cas.cz.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Cwiklik
authors.affiliation: J. Heyrovský Institute of Physical Chemistry of the Academy of Sciences of the Czech Republic, v. v. i., Dolejškova 2155/3, 182 23 Prague 8, Czech Republic. Electronic address: jana.humpolickova@jh-inst.cas.cz.
authors.forename: Jana
authors.initials: J
authors.lastname: Humpolíčková
authors.affiliation: J. Heyrovský Institute of Physical Chemistry of the Academy of Sciences of the Czech Republic, v. v. i., Dolejškova 2155/3, 182 23 Prague 8, Czech Republic. Electronic address: martin.hof@jh-inst.cas.cz.
authors.forename: Martin
authors.initials: M
authors.lastname: Hof
mesh_terms: D002462:Cell Membrane / Q000737:chemistry; D002784:Cholesterol / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008051:Lipid Bilayers / Q000737:chemistry*; D008563:Membrane Lipids / Q000737:chemistry*; D056004:Molecular Dynamics Simulation; D010084:Oxidation-Reduction; D010743:Phospholipids / Q000737:chemistry*
chemical_list: D008051:Lipid Bilayers; D008563:Membrane Lipids; D010743:Phospholipids; D002784:Cholesterol
keywords: Electron paramagnetic resonance; Fluorescence correlation spectroscopy; Lateral diffusion; Molecular dynamics simulations; Oxidized lipids