title: RNAi screening in Drosophila cells and in vivo.
abstract: Here, I discuss how RNAi screening can be used effectively to uncover gene function. Specifically, I discuss the types of high-throughput assays that can be done in Drosophila cells and in vivo, RNAi reagent design and available reagent collections, automated screen pipelines, analysis of screen results, and approaches to RNAi results verification.
authors.affiliation: Drosophila RNAi Screening Center, Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, United States. Electronic address: stephanie_mohr@hms.harvard.edu.
authors.forename: Stephanie E
authors.initials: SE
authors.lastname: Mohr
mesh_terms: D000818:Animals; D015509:Developmental Biology / Q000379:methods*; D004330:Drosophila; D023281:Genomics; D057166:High-Throughput Screening Assays / Q000379:methods*; D034622:RNA Interference
keywords: Cell-based assays; Drosophila; Functional genomics; High-throughput screening; In vivo assays; RNAi