title: Overexpression of Fas and FasL is associated with infectious complications and severity of experimental severe acute pancreatitis by promoting apoptosis of lymphocytes.
abstract: This study investigated the relationship of Fas and Fas ligand (FasL) expression and apoptosis of lymphocytes in relation to the pathogenic immune response and infectious complications observed in experimental severe acute pancreatitis in mice. Forty male Balb/c mice were randomly divided into control, mild (MAP), and severe acute pancreatitis (SAP) groups. Overexpression of Fas/FasL messenger ribonucleic acid (mRNA) and protein was observed in spleen-derived lymphocytes in SAP (p < 0.01). Apoptosis of these resulted in a depletion of circulating lymphocytes in this group (p < 0.05). A further significant change in the SAP group with infectious complications was observed. A positive relationship was found between the Fas/FasL expression and lymphocyte apoptosis, and negative relationships were observed between Fas/FasL expression and CD4(+) and CD19(+) lymphocytes and the CD4(+)/CD8(+) ratio in SAP mice (p < 0.01). The results suggest that the overexpression of Fas/FasL is associated with infectious complications and severity of experimental severe acute pancreatitis by promoting apoptosis of lymphocytes.
authors.affiliation: Intensive Care Unit, Affiliated Hospital of Youjiang Medical University for Nationalities, No. 18 Zhongshan Road II, 533000, Baise, Guangxi Zhuang Autonomous Region, China, liaopinhu@gmail.com.
authors.forename: Liao
authors.initials: L
authors.lastname: Pinhu
authors.forename: Yueqiu
authors.initials: Y
authors.lastname: Qin
authors.forename: Bin
authors.initials: B
authors.lastname: Xiong
authors.forename: Yanwu
authors.initials: Y
authors.lastname: You
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Suren R
authors.initials: SR
authors.lastname: Sooranna
mesh_terms: D000818:Animals; D018941:Antigens, CD19 / Q000096:biosynthesis / Q000378:metabolism; D017209:Apoptosis; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000473:pathology*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000473:pathology*; D002108:Ceruletide / Q000737:chemistry; D053222:Fas Ligand Protein / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000737:chemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010195:Pancreatitis / Q000276:immunology* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013154:Spleen / Q000378:metabolism; D019014:fas Receptor / Q000378:metabolism*
chemical_list: D018941:Antigens, CD19; D053222:Fas Ligand Protein; C491899:Fas protein, mouse; C505658:Fasl protein, mouse; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D019014:fas Receptor; D002108:Ceruletide