title: [Effects of pioglitazone on myocardial peroxisome proliferator-activated receptor gamma co-activator lα expression in rats with myocardial ischemia/reperfusion injury].
abstract: OBJECTIVE
  To investigate the effects of pioglitazone on the expression of peroxisome proliferator-activated receptor gamma co-activator lα (PGC-lα) in rat myocardium following myocardial ischemia/reperfusion (I/R) injury.
  
  
  METHODS
  Twenty-four SD rats were randomly divided into 4 equal groups, namely I/R group, pioglitazone (5 mg/kg daily) group, pioglitazone (10 mg/kg daily) group, and pioglitazone (10 mg/kg) +peroxisome proliferator-activated receptor γ (PPARγ)-specific antagonist GW9662 group. Myocardial I/R injury was induced by ligation of the left anterior descending coronary artery for 30 min and reperfusion for 120 min. Myocardial apoptosis following I/R injury was examined with TUNEL assay; RT-PCR and Western blotting were employed to detect the expression of PGC-lα mRNA and protein, respectively.
  
  
  RESULTS
  Pioglitazone treatment significantly suppressed myocardial apoptosis (21.4%∓8.8%,17.3%∓8.7%, 40.1%∓12.3%, P<0.05) following I/R injury and up-regulated myocardial PGC-lα expression at both the mRNA and protein levels (P<0.05), but these effects were antagonized by GW9662 (P<0.05).
  
  
  CONCLUSIONS
  Pioglitazone can inhibit myocardial apoptosis induced by I/R injury and up-regulate myocardial PGC-lα expression, and these effects are mediated by PPARγ.
authors.affiliation: Second Department of Geriatric Cardiology, General Hospital of PLA, Beijing 100853, China.E-mail: linlinshen1995@126.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Shen
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Ye
mesh_terms: D000813:Anilides / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D000077205:Pioglitazone; D051381:Rats; D017207:Rats, Sprague-Dawley; D045162:Thiazolidinediones / Q000494:pharmacology*; D014157:Transcription Factors / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: C457499:2-chloro-5-nitrobenzanilide; D000813:Anilides; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C501429:Ppargc1a protein, rat; D045162:Thiazolidinediones; D014157:Transcription Factors; D000077205:Pioglitazone