title: Community structure and succession regulation of fungal consortia in the lignocellulose-degrading process on natural biomass.
abstract: The study aims to investigate fungal community structures and dynamic changes in forest soil lignocellulose-degrading process. rRNA gene clone libraries for the samples collected in different stages of lignocellulose degradation process were constructed and analyzed. A total of 26 representative RFLP types were obtained from original soil clone library, including Mucoromycotina (29.5%), unclassified Zygomycetes (33.5%), Ascomycota (32.4%), and Basidiomycota (4.6%). When soil accumulated with natural lignocellulose, 16 RFLP types were identified from 8-day clone library, including Basidiomycota (62.5%), Ascomycota (36.1%), and Fungi incertae sedis (1.4%). After enrichment for 15 days, identified 11 RFLP types were placed in 3 fungal groups: Basidiomycota (86.9%), Ascomycota (11.5%), and Fungi incertae sedis (1.6%). The results showed richer, more diversity and abundance fungal groups in original forest soil. With the degradation of lignocellulose, fungal groups Mucoromycotina and Ascomycota decreased gradually, and wood-rotting fungi Basidiomycota increased and replaced the opportunist fungi to become predominant group. Most of the fungal clones identified in sample were related to the reported lignocellulose-decomposing strains. Understanding of the microbial community structure and dynamic change during natural lignocellulose-degrading process will provide us with an idea and a basis to construct available commercial lignocellulosic enzymes or microbial complex.
authors.affiliation: Engineering Research Center of Industrial Microbiology of Ministry of Education, and College of Life Sciences, Fujian Normal University, Fuzhou 350108, China.
authors.forename: Baoyu
authors.identifier: 0000-0003-3215-5720
authors.initials: B
authors.lastname: Tian
authors.affiliation: Engineering Research Center of Industrial Microbiology of Ministry of Education, and College of Life Sciences, Fujian Normal University, Fuzhou 350108, China.
authors.forename: Chunxiang
authors.initials: C
authors.lastname: Wang
authors.affiliation: Engineering Research Center of Industrial Microbiology of Ministry of Education, and College of Life Sciences, Fujian Normal University, Fuzhou 350108, China.
authors.forename: Ruirui
authors.initials: R
authors.lastname: Lv
authors.affiliation: Engineering Research Center of Industrial Microbiology of Ministry of Education, and College of Life Sciences, Fujian Normal University, Fuzhou 350108, China.
authors.forename: Junxiong
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Engineering Research Center of Industrial Microbiology of Ministry of Education, and College of Life Sciences, Fujian Normal University, Fuzhou 350108, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Engineering Research Center of Industrial Microbiology of Ministry of Education, and College of Life Sciences, Fujian Normal University, Fuzhou 350108, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Engineering Research Center of Industrial Microbiology of Ministry of Education, and College of Life Sciences, Fujian Normal University, Fuzhou 350108, China.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Jin
authors.affiliation: Engineering Research Center of Industrial Microbiology of Ministry of Education, and College of Life Sciences, Fujian Normal University, Fuzhou 350108, China.
authors.forename: Mengli
authors.initials: M
authors.lastname: Wang
authors.affiliation: Engineering Research Center of Industrial Microbiology of Ministry of Education, and College of Life Sciences, Fujian Normal University, Fuzhou 350108, China.
authors.forename: Yongxia
authors.initials: Y
authors.lastname: Ye
authors.affiliation: Engineering Research Center of Industrial Microbiology of Ministry of Education, and College of Life Sciences, Fujian Normal University, Fuzhou 350108, China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Huang
authors.affiliation: Engineering Research Center of Industrial Microbiology of Ministry of Education, and College of Life Sciences, Fujian Normal University, Fuzhou 350108, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
mesh_terms: D018533:Biomass; D017753:Ecosystem; D005658:Fungi / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008031:Lignin / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D012111:Residence Characteristics; D012988:Soil Microbiology
chemical_list: C036909:lignocellulose; D008031:Lignin