title: Gene silencing and gene expression in phytopathogenic fungi using a plant virus vector.
abstract: RNA interference (RNAi) is a powerful approach for elucidating gene functions in a variety of organisms, including phytopathogenic fungi. In such fungi, RNAi has been induced by expressing hairpin RNAs delivered through plasmids, sequences integrated in fungal or plant genomes, or by RNAi generated in planta by a plant virus infection. All these approaches have some drawbacks ranging from instability of hairpin constructs in fungal cells to difficulties in preparing and handling transgenic plants to silence homologous sequences in fungi grown on these plants. Here we show that RNAi can be expressed in the phytopathogenic fungus Colletotrichum acutatum (strain C71) by virus-induced gene silencing (VIGS) without a plant intermediate, but by using the direct infection of a recombinant virus vector based on the plant virus, tobacco mosaic virus (TMV). We provide evidence that a wild-type isolate of TMV is able to enter C71 cells grown in liquid medium, replicate, and persist therein. With a similar approach, a recombinant TMV vector carrying a gene for the ectopic expression of the green fluorescent protein (GFP) induced the stable silencing of the GFP in the C. acutatum transformant line 10 expressing GFP derived from C71. The TMV-based vector also enabled C. acutatum to transiently express exogenous GFP up to six subcultures and for at least 2 mo after infection, without the need to develop transformation technology. With these characteristics, we anticipate this approach will find wider application as a tool in functional genomics of filamentous fungi.
authors.affiliation: Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università degli Studi di Bari Aldo Moro, 70126 Bari, Italy.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Mascia
authors.forename: Franco
authors.initials: F
authors.lastname: Nigro
authors.forename: Alì
authors.initials: A
authors.lastname: Abdallah
authors.forename: Massimo
authors.initials: M
authors.lastname: Ferrara
authors.forename: Angelo
authors.initials: A
authors.lastname: De Stradis
authors.forename: Roberto
authors.initials: R
authors.lastname: Faedda
authors.forename: Peter
authors.initials: P
authors.lastname: Palukaitis
authors.forename: Donato
authors.initials: D
authors.lastname: Gallitelli
mesh_terms: D020231:Colletotrichum / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D005822:Genetic Vectors; D023281:Genomics / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D014027:Tobacco Mosaic Virus; D014162:Transfection / Q000379:methods
chemical_list: D034741:RNA, Small Interfering
keywords: host species jump; plant pathogen adaptation; transfection