title: Increased survival after irradiation followed by regeneration of bone marrow stromal cells with a novel thiol-based radioprotector.
abstract: OBJECTIVE
  To investigate the survival of laboratory rats after irradiation and to study the cellularity of their bone marrow and the multipotential mesenchymal stem cells (BM-MSCs) in groups treated with or without a new thiol-based radioprotector (GM2011).
  
  
  METHODS
  Animals were irradiated by a Cobalt gamma source at 6.7 Gy. Treated animals were given i.p. GM2011 30 minutes before and 3 and 7 hours after irradiation. Controls consisted of sham irradiated animals without treatment and animals treated without irradiation. After 30 days post-irradiation, animals were sacrificed and bone marrow cells were prepared from isolated femurs. A colony forming unit-fibroblast (CFU-F) assay was performed to obtain the number of BM-MSCs.
  
  
  RESULTS
  In the treated group, 87% of animals survived, compared to only 30% in the non-treated irradiated group. Irradiation induced significant changes in the bone marrow of the treated rats (total bone marrow cellularity was reduced by~60%--from 63 to 28 cells × 10(6)/femur and the frequency of the CFU-F per femur by~70% - from 357 to 97), however GL2011 almost completely prevented the suppressive effect observed on day 30 post-irradiation (71 cells × 10(6)/femur and 230 CFU-F/femur).
  
  
  CONCLUSIONS
  Although the irradiation dosage was relatively high, GL2011 acted as a very effective new radioprotector. The recovery of the BN-MSCs and their counts support the effectiveness of the studied radioprotector.
authors.forename: Ivana
authors.initials: I
authors.lastname: Okić-Djordjević
authors.forename: Drenka
authors.initials: D
authors.lastname: Trivanović
authors.forename: Miloš
authors.initials: M
authors.lastname: Jovanović
authors.forename: Marija
authors.initials: M
authors.lastname: Ignjatović
authors.forename: Bojana
authors.initials: B
authors.lastname: Šećerov
authors.forename: Miloš
authors.initials: M
authors.lastname: Mojović
authors.forename: Diana
authors.initials: D
authors.lastname: Bugarski
authors.forename: Goran
authors.initials: G
authors.lastname: Bačić
authors.affiliation: Pavle R. Andjus, Faculty of Biology University of Belgrade, Studentski trg 12, 11000 Belgrade, Serbia, pandjus@bio.bg.ac.rs.
authors.forename: Pavle R
authors.initials: PR
authors.lastname: Andjus
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000528:radiation effects*; D003037:Cobalt Radioisotopes; D003114:Colony-Forming Units Assay; D008297:Male; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D011833:Radiation Injuries, Experimental / Q000209:etiology / Q000378:metabolism / Q000401:mortality / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000502:physiology*; D015996:Survival Rate; D014916:Whole-Body Irradiation
chemical_list: D003037:Cobalt Radioisotopes; D011837:Radiation-Protective Agents