title: Trypsin-catalyzed deltamethrin degradation.
abstract: To explore if trypsin could catalyze the degradation of non-protein molecule deltamethrin, we compared in vitro hydrolytic reactions of deltamethrin in the presence and absence of trypsin with ultraviolet-visible (UV/Vis) spectrophotometry and gas chromatography-mass spectrometry (GC/MS). In addition, acute oral toxicity of the degradation products was determined in Wistar rats. The results show that the absorption peak of deltamethrin is around 264 nm, while the absorption peaks of deltamethrin degradation products are around 250 nm and 296 nm. In our GC setting, the retention time of undegraded deltamethrin was 37.968 min, while those of deltamethrin degradation products were 15.289 min and 18.730 min. The LD50 of deltamethrin in Wistar rats is 55 mg/kg, while that of deltamethrin degradation products is 3358 mg/kg in female rats and 1045 mg/kg in male rates (61-fold and 19-fold reductions in toxicity), suggesting that trypsin could directly degrade deltamethrin, which significantly reduces the toxicity of deltamethrin. These results expand people's understanding of the functions of proteases and point to potential applications of trypsin as an attractive agent to control residual pesticides in the environment and on agricultural products.
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, China; Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, China.
authors.forename: Chunrong
authors.initials: C
authors.lastname: Xiong
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, China; Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, China.
authors.forename: Fujin
authors.initials: F
authors.lastname: Fang
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, China; Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, China; Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, China.
authors.forename: Qinggui
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, China; Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, China.
authors.forename: Ji
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, China; Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhou
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, China; Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Shen
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, China; Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, China; Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, China; Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, China.
authors.forename: Donghui
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, China; Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, China.
authors.forename: Changliang
authors.initials: C
authors.lastname: Zhu
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002384:Catalysis; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D007306:Insecticides / Q000008:administration & dosage / Q000378:metabolism*; D008297:Male; D009570:Nitriles / Q000008:administration & dosage / Q000378:metabolism*; D011722:Pyrethrins / Q000008:administration & dosage / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013056:Spectrophotometry, Ultraviolet; D018675:Toxicity Tests; D014357:Trypsin / Q000378:metabolism*
chemical_list: D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin; D014357:Trypsin