title: Development of a new real-time polymerase chain reaction assay to detect Duck adenovirus A DNA and application to samples from Swiss poultry flocks.
abstract: Between 2008 and 2012, commercial Swiss layer and layer breeder flocks experiencing problems in laying performance were sampled and tested for infection with Duck adenovirus A (DAdV-A; previously known as Egg drop syndrome 1976 virus). Organ samples from birds sent for necropsy as well as blood samples from living animals originating from the same flocks were analyzed. To detect virus-specific DNA, a newly developed quantitative real-time polymerase chain reaction method was applied, and the presence of antibodies against DAdV-A was tested using a commercially available enzyme-linked immunosorbent assay. In 5 out of 7 investigated flocks, viral DNA was detected in tissues. In addition, antibodies against DAdV-A were detected in all of the flocks.
authors.affiliation: 1Andrea Vögtlin, Institute of Virology and Immunology (IVI), Sensemattstraße 293, 3147 Mittelhäusern, Switzerland. andrea.voegtlin@ivi.admin.ch.
authors.forename: Martina
authors.initials: M
authors.lastname: Schybli
authors.forename: Brigitte
authors.initials: B
authors.lastname: Sigrist
authors.forename: Michael
authors.initials: M
authors.lastname: Hess
authors.forename: Bart
authors.initials: B
authors.lastname: van Leerdam
authors.forename: Richard K
authors.initials: RK
authors.lastname: Hoop
authors.forename: Andrea
authors.initials: A
authors.lastname: Vögtlin
mesh_terms: D000257:Adenoviridae Infections / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D046808:Atadenovirus / Q000235:genetics / Q000302:isolation & purification*; D002645:Chickens; D004279:DNA, Viral / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D011201:Poultry Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary*; D012680:Sensitivity and Specificity; D013047:Specific Pathogen-Free Organisms; D013557:Switzerland / Q000453:epidemiology
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral
keywords: Duck adenovirus A; Egg drop syndrome 1976 virus; Switzerland; enzyme-linked immunosorbent assay; laying hens; real-time polymerase chain reaction assay