title: Multiplex analysis of polyA-linked sequences (MAPS): an RNA-seq strategy to profile poly(A+) RNA.
abstract: We summarize 12 experimental methods that have been developed for profiling gene expression by focusing on the 3'-end of poly(A+) mRNA, distilling both common and unique features. Of this family of methods, we provide detailed protocol for MAPS, a method we believe is the simplest and most cost-effective for profiling gene expression and quantifying alternative polyadenylation events by oligo-dT priming followed by random priming and deep sequencing. This method also enables library multiplexing by using a set of bar coding primers during PCR amplification. We also provide a general guideline for analysis of the data generated by MAPS by using the software package maps3end.
authors.affiliation: Department of Cellular and Molecular Medicine, Institute for Genomic Medicine, University of California, Room 239, San Diego, La Jolla, CA, 92093-0651, USA.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
authors.forename: Hai-Ri
authors.initials: HR
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Huang
authors.forename: Ge
authors.initials: G
authors.lastname: Jin
authors.forename: Xiang-Dong
authors.initials: XD
authors.lastname: Fu
mesh_terms: D020869:Gene Expression Profiling; D059014:High-Throughput Nucleotide Sequencing; D011061:Poly A / Q000737:chemistry / Q000378:metabolism*; D026723:Polyadenylation / Q000502:physiology; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D012984:Software
chemical_list: D011061:Poly A; D012313:RNA