title: Non-viral gene therapy for bone tissue engineering.
abstract: The possibilities of using gene therapy for bone regeneration have been extensively investigated. Improvements in the design of new transfection agents, combining vectors and delivery/release systems to diminish cytotoxicity and increase transfection efficiencies have led to several successful in vitro, ex vivo and in vivo strategies. These include growth factor or short interfering ribonucleic acid (siRNA) delivery, or even enzyme replacement therapies, and have led to increased osteogenic differentiation and bone formation in vivo. These results provide optimism to consider use in humans with some of these gene-delivery strategies in the near future.
authors.affiliation: a Department of Orthopaedics , UMC Utrecht , Utrecht , The Netherlands.
authors.forename: Fiona
authors.initials: F
authors.lastname: Wegman
authors.forename: F Cumhur
authors.initials: FC
authors.lastname: Oner
authors.forename: Wouter J A
authors.initials: WJ
authors.lastname: Dhert
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Alblas
mesh_terms: D001861:Bone Regeneration / Q000235:genetics*; D002454:Cell Differentiation / Q000235:genetics; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics*; D023822:Tissue Engineering; D014162:Transfection / Q000379:methods