title: Re-inoculation strategies enhance the degradation of emerging pollutants in fungal bioaugmentation of sewage sludge.
abstract: The use of Trametes versicolor has been partially successful in the removal of some pharmaceuticals from sewage sludge in laboratory-scale biopile systems. The application of two strategies for the re-inoculation of biomass was assessed during the fungal bioaugmentation of non-sterile sludge (42-d treatment) as an approach to improve the elimination of pharmaceuticals and other groups of emerging pollutants. Globally, the re-inoculation of biopiles with blended mycelium exerted a major effect on the removal of pharmaceuticals (86%), brominated-flame-retardants (81%) and UV filters (80%) with respect to the re-inoculation with additional lignocellulosic substrate colonized by the fungus (69-67-22%). The performance was better than that of the analogous non-re-inoculated systems that were assayed previously for the removal of pharmaceuticals. The results demonstrate the ability of T. versicolor to remove a wide spectrum of emerging micropollutants under non-sterile conditions, while re-inoculation appears to be a useful step to improve the fungal treatment of sludge.
authors.affiliation: Institut de Química Avançada de Catalunya (IQAC) CSIC, Jordi Girona 18-26, 08034 Barcelona, Spain. Electronic address: carlos.rodriguezrodriguez@ucr.ac.cr.
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Rodríguez-Rodríguez
authors.affiliation: Catalan Institute for Water Research (ICRA), Scientific and Technological Park of the University of Girona, H2O Building, Emili Grahit 101, 17003 Girona, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Lucas
authors.affiliation: Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research (IDAEA), Spanish Council for Scientific Research (CSIC), Jordi Girona 18-26, E-08034 Barcelona, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Barón
authors.affiliation: Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research (IDAEA), Spanish Council for Scientific Research (CSIC), Jordi Girona 18-26, E-08034 Barcelona, Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: Gago-Ferrero
authors.affiliation: Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research (IDAEA), Spanish Council for Scientific Research (CSIC), Jordi Girona 18-26, E-08034 Barcelona, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Molins-Delgado
authors.affiliation: Catalan Institute for Water Research (ICRA), Scientific and Technological Park of the University of Girona, H2O Building, Emili Grahit 101, 17003 Girona, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Rodríguez-Mozaz
authors.affiliation: Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research (IDAEA), Spanish Council for Scientific Research (CSIC), Jordi Girona 18-26, E-08034 Barcelona, Spain.
authors.forename: Ethel
authors.initials: E
authors.lastname: Eljarrat
authors.affiliation: Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research (IDAEA), Spanish Council for Scientific Research (CSIC), Jordi Girona 18-26, E-08034 Barcelona, Spain.
authors.forename: M Silvia
authors.initials: MS
authors.lastname: Díaz-Cruz
authors.affiliation: Catalan Institute for Water Research (ICRA), Scientific and Technological Park of the University of Girona, H2O Building, Emili Grahit 101, 17003 Girona, Spain; Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research (IDAEA), Spanish Council for Scientific Research (CSIC), Jordi Girona 18-26, E-08034 Barcelona, Spain.
authors.forename: Damià
authors.initials: D
authors.lastname: Barceló
authors.affiliation: Institut de Química Avançada de Catalunya (IQAC) CSIC, Jordi Girona 18-26, 08034 Barcelona, Spain.
authors.forename: Glòria
authors.initials: G
authors.lastname: Caminal
authors.affiliation: Departament d'Enginyeria Química, Escola d'Enginyeria, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Vicent
mesh_terms: D001673:Biodegradation, Environmental; D052918:Environmental Restoration and Remediation / Q000379:methods*; D005374:Filtration; D005411:Flame Retardants / Q000302:isolation & purification; D004364:Pharmaceutical Preparations / Q000302:isolation & purification; D012722:Sewage / Q000382:microbiology*; D013997:Time Factors; D055454:Trametes / Q000378:metabolism*; D014466:Ultraviolet Rays; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*
chemical_list: D005411:Flame Retardants; D004364:Pharmaceutical Preparations; D012722:Sewage; D014874:Water Pollutants, Chemical
keywords: Bioaugmentation; Bioremediation; Emerging pollutants; Sewage sludge; Trametes versicolor