title: An improved technique for the rapid chemical characterisation of bacterial terpene cyclases.
abstract: A derivative of the pET28c(+) expression vector was constructed. It contains a yeast replication system (2μ origin of replication) and a yeast selectable marker (URA3), and can be used for gene cloning in yeast by efficient homologous recombination, and for heterologous expression in E. coli. The vector was used for the expression and chemical characterisation of three bacterial terpene cyclases.
authors.affiliation: Institut für Organische Chemie, TU Braunschweig, Hagenring 30, 38106 Braunschweig (Germany). j.dickschat@tu-bs.de.
authors.forename: Jeroen S
authors.initials: JS
authors.lastname: Dickschat
authors.forename: Khomaizon A K
authors.initials: KA
authors.lastname: Pahirulzaman
authors.forename: Patrick
authors.initials: P
authors.lastname: Rabe
authors.forename: Tim A
authors.initials: TA
authors.lastname: Klapschinski
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000378:metabolism; D005822:Genetic Vectors / Q000378:metabolism; D016930:Saccharopolyspora / Q000201:enzymology; D013237:Stereoisomerism; D013729:Terpenes / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D013729:Terpenes; D019883:Alkyl and Aryl Transferases; C108877:terpene synthase
keywords: GC/MS; actinomycetes; biosynthesis; enzymes; heterologous expression; terpene cyclases