title: Evaluation of urinary speciated arsenic in NHANES: issues in interpretation in the context of potential inorganic arsenic exposure.
abstract: Urinary dimethylarsinic acid (DMA) and monomethylarsonic acid (MMA) are among the commonly used biomarkers for inorganic arsenic (iAs) exposure, but may also arise from seafood consumption and organoarsenical pesticide applications. We examined speciated urinary arsenic data from National Health and Nutrition Examination Survey (NHANES) 2009-2010 cycle to assess potential correlations among urinary DMA, MMA, and the organic arsenic species arsenobetaine. Urinary DMA and MMA were positively associated with urinary arsenobetaine, suggesting direct exposure to these species in seafood or metabolism of organic arsenicals to these species, although the biomonitoring data do not directly identify the sources of exposure. The magnitude of association was much larger for DMA than for MMA. The secondary methylation index (SMI, ratio of urinary DMA to MMA) observed in the NHANES program likewise is much higher in persons with detected arsenobetaine than in those without, again suggesting that direct DMA exposure is co-occurring with exposure to arsenobetaine. Urinary MMA was less correlated with organic arsenic exposures than DMA and, therefore, may be a more reliable biomarker for iAs exposure in the general US population. However, given the associations between both MMA and DMA and organic arsenic species in urine, interpretations of the urinary arsenic concentrations observed in the NHANES in the context of potential arsenic exposure should be made cautiously.
authors.affiliation: Summit Toxicology, LLP, Falls Church, VA, USA. Electronic address: laylward@summittoxicology.com.
authors.forename: Lesa L
authors.initials: LL
authors.lastname: Aylward
authors.affiliation: Office of Water, US Environmental Protection Agency, Washington, DC, USA.
authors.forename: Santhini
authors.initials: S
authors.lastname: Ramasamy
authors.affiliation: Summit Toxicology, LLP, Lyons, CO, USA.
authors.forename: Sean M
authors.initials: SM
authors.lastname: Hays
authors.affiliation: Office of Science Policy, Office of Research and Development, US Environmental Protection Agency, Washington, DC, USA.
authors.forename: Rita
authors.initials: R
authors.lastname: Schoeny
authors.affiliation: Summit Toxicology, LLP, Orange Village, OH, USA.
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Kirman
mesh_terms: D001151:Arsenic / Q000652:urine*; D001152:Arsenicals / Q000652:urine; D015415:Biomarkers / Q000652:urine; D002101:Cacodylic Acid / Q000652:urine; D004781:Environmental Exposure / Q000009:adverse effects; D004784:Environmental Monitoring / Q000379:methods; D006801:Humans; D009749:Nutrition Surveys / Q000379:methods; D017747:Seafood / Q000009:adverse effects; D014874:Water Pollutants, Chemical / Q000652:urine
chemical_list: D001152:Arsenicals; D015415:Biomarkers; D014874:Water Pollutants, Chemical; D002101:Cacodylic Acid; C020300:monomethylarsonic acid; D001151:Arsenic; C038992:arsenobetaine
keywords: Arsenic; Biomonitoring; Exposure assessment; NHANES