title: [Detection of replication sites in the nuclei of plant cells using semithin sections].
abstract: A novel approach for the detection of replication sites in plant cells nuclei is described. Included nucleotide (EdU) was detected using "click"-chemistry in semithin sections of the material embedded in acrylic resin. The usage of the protocol introduced allows: 1) to preserve the intact morphology of cells, 2) to work with any tissue, and 3) to obtain high-resolution microscopy (especially, axial).
authors.forename: M A
authors.initials: MA
authors.lastname: Kuznetsova
authors.forename: E V
authors.initials: EV
authors.lastname: Sheval'
mesh_terms: D002467:Cell Nucleus; D002843:Chromatin / Q000235:genetics; D004261:DNA Replication / Q000235:genetics*; D018551:Solanum lycopersicum / Q000166:cytology*; D059828:Plant Cells
chemical_list: D002843:Chromatin