title: Activation of transient receptor potential melastatin 8 reduces ultraviolet B-induced prostaglandin E2 production in keratinocytes.
abstract: Transient receptor potential melastatin 8 (TRPM8) is a member of the TRP family, and is activated at temperatures below 22°C, or by cooling compounds such as menthol. In this study, it was found that a new role of TRPM8 activation on prostaglandin E2 (PGE2), an inflammatory cytokine and dendritogenesis stimulator of normal human melanocytes. Normal human keratinocytes were pretreated with menthol or incubated at 22°C for TRPM8 activation before ultraviolet (UV)-B irradiation. To examine the specificity between TRPM8 activation and PGE2 release, we inhibited TRPM8 with the antagonist (capsazepine), or introduced TRPM8 siRNA for a gene silencing experiment. UV-B irradiation significantly induced PGE2 release in normal human keratinocytes. Interestingly, activation of TRPM8 at 22°C or with menthol inhibited UV-B-induced PGE2 release. The effect of the TRPM8 agonist was completely blocked by pretreatment with the TRPM8 antagonist, capsazepine. When TRPM8 expression was suppressed by siRNA, UV-B irradiation still upregulated PGE2 in keratinocytes, but pretreatment of menthol or low temperature did not inhibit UV-B-induced PGE2. In conclusion, the activation of TRPM8 inhibits UV-B-induced PGE2 production in keratinocytes, and the activation of TRPM8 may reduce inflammatory responses in skin.
authors.affiliation: Skin Research Institute, Amorepacific R&D Center, Yongin, South Korea.
authors.forename: Nok-Hyun
authors.initials: NH
authors.lastname: Park
authors.forename: Yong-Joo
authors.initials: YJ
authors.lastname: Na
authors.forename: Hyang-Tae
authors.initials: HT
authors.lastname: Choi
authors.forename: Jun-Cheol
authors.initials: JC
authors.lastname: Cho
authors.forename: Hae-Kwang
authors.initials: HK
authors.lastname: Lee
mesh_terms: D002478:Cells, Cultured; D015232:Dinoprostone / Q000096:biosynthesis*; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism / Q000528:radiation effects*; D050053:TRPM Cation Channels / Q000378:metabolism*; D014466:Ultraviolet Rays
chemical_list: D050053:TRPM Cation Channels; C425966:TRPM8 protein, human; D015232:Dinoprostone
keywords: keratinocyte; prostaglandin E2; transient receptor potential melastatin 8; ultraviolet B