title: The orphan nuclear receptor SF-1 is involved in the effect of PCBs, DDT, and DDE on the secretion of steroid hormones and oxytocin from bovine luteal cells during the estrous cycle in vitro.
abstract: The orphan receptor steroidogenic factor-1 (SF-1) is involved in the regulation of ovarian steroidogenesis in cows. It is hypothesized that estrogen-like chlorinated compounds might affect SF-1, and thus impair the function of the ovary. Bovine luteal cells from the estrous cycle (Days: 1-5, 6-10, 11-15, and 16-19) were treated for 50 hours with DDT, 1,1-dichloro-2,2-bis(4-chlorophenyl)ethene, 3,3'4,4'-tetrachlorobiphenyl or 2'2'4,4',5,5'-hexachlorobiphenyl (each at a dose of 10 ng/mL). Luteal cells were also treated with 4-(heptyloxy)phenol (1 × 10(-7) M), an SF-1 agonist, and F0160 (1 × 10(-6) M), an SF-1 blocker, jointly or separately. The secretion of progesterone and oxytocin and the expression of oxytocin precursor (NP-I/OT) messenger RNA were increased (P < 0.05) by all studied xenobiotics and 4-(heptyloxy)phenol, although they were inhibited (P < 0.05) by F0160. However, the xenobiotics did not affect (P > 0.05) SF-1 messenger RNA expression. In summary, SF-1 is involved in the adverse effect of chlorinated xenobiotics on the regulation of the bovine CL.
authors.affiliation: Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Mlynarczuk
authors.affiliation: Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.
authors.forename: M H
authors.initials: MH
authors.lastname: Wrobel
authors.affiliation: Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland. Electronic address: janko@pan.olsztyn.pl.
authors.forename: J
authors.initials: J
authors.lastname: Kotwica
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003634:DDT / Q000633:toxicity; D003633:Dichlorodiphenyl Dichloroethylene / Q000633:toxicity; D004785:Environmental Pollutants / Q000633:toxicity*; D030762:Estrous Cycle / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D008184:Luteal Cells / Q000378:metabolism*; D010121:Oxytocin / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000633:toxicity; D054339:Steroidogenic Factor 1 / Q000378:metabolism*; D013256:Steroids / Q000378:metabolism
chemical_list: D004785:Environmental Pollutants; D054339:Steroidogenic Factor 1; D013256:Steroids; D003633:Dichlorodiphenyl Dichloroethylene; D010121:Oxytocin; D003634:DDT; D011078:Polychlorinated Biphenyls
keywords: Chlorinated xenobiotics; Cow; Luteal cell; Oxytocin; Progesterone; Steroidogenic factor-1