title: SAW1 is required for SDSA double-strand break repair in S. cerevisiae.
abstract: SAW1, coding for Saw1, is required for single-strand annealing (SSA) DNA double-strand break (DSB) repair in Saccharomycescerevisiae. Saw1 physically associates with Rad1 and Rad52 and recruits the Rad1-Rad10 endonuclease. Herein we show by fluorescence microscopy that SAW1 is similarly required for recruitment of Rad10 to sites of Synthesis-Dependent Strand Annealing (SDSA) and associates with sites of SDSA repair in a manner temporally overlapped with Rad10. The magnitude of induction of colocalized Saw1-CFP/Rad10-YFP/DSB-RFP foci in SDSA is more dramatic in S and G2 phase cells than in M phase, consistent with the known mechanism of SDSA. We observed a substantial fraction of foci in which Rad10 was localized to the repair site without Saw1, but few DSB sites that contained Saw1 without Rad10. Together these data are consistent with a model in which Saw1 recruits Rad1-Rad10 to SDSA sites, possibly even binding as a protein-protein complex, but departs the repair site in advance of Rad1-Rad10.
authors.affiliation: Department of Chemistry and Biochemistry, California State University Northridge, 18111 Nordhoff ST, Northridge, CA 91330-8262, United States.
authors.forename: Graciel
authors.initials: G
authors.lastname: Diamante
authors.affiliation: Department of Chemistry and Biochemistry, California State University Northridge, 18111 Nordhoff ST, Northridge, CA 91330-8262, United States.
authors.forename: Claire
authors.initials: C
authors.lastname: Phan
authors.affiliation: Department of Chemistry and Biochemistry, California State University Northridge, 18111 Nordhoff ST, Northridge, CA 91330-8262, United States.
authors.forename: Angie S
authors.initials: AS
authors.lastname: Celis
authors.affiliation: Department of Chemistry and Biochemistry, California State University Northridge, 18111 Nordhoff ST, Northridge, CA 91330-8262, United States.
authors.forename: Jonas
authors.initials: J
authors.lastname: Krueger
authors.affiliation: Department of Chemistry and Biochemistry, California State University Northridge, 18111 Nordhoff ST, Northridge, CA 91330-8262, United States.
authors.forename: Eric P
authors.initials: EP
authors.lastname: Kelson
authors.affiliation: Department of Chemistry and Biochemistry, California State University Northridge, 18111 Nordhoff ST, Northridge, CA 91330-8262, United States. Electronic address: paula.fischhaber@csun.edu.
authors.forename: Paula L
authors.initials: PL
authors.lastname: Fischhaber
mesh_terms: D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000032:analysis / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; C579349:Saw1 protein, S cerevisiae; C064162:RAD10 protein, S cerevisiae; D015719:Single-Strand Specific DNA and RNA Endonucleases
keywords: Double strand break repair; Rad10; Saw1; Synthesis Dependent Strand Annealing