title: An animal component free medium that promotes the growth of various animal cell lines for the production of viral vaccines.
abstract: IPT-AFM is a proprietary animal component free medium that was developed for rabies virus (strain LP 2061) production in Vero cells. In the present work, we demonstrated the versatility of this medium and its ability to sustain the growth of other cell lines and different virus strains. Here, three models were presented: Vero cells/rabies virus (strain LP 2061), MRC-5 cells/measles virus (strain AIK-C) and BHK-21 cells/rabies virus (strain PV-BHK21). The cell lines were first adapted to grow in IPT-AFM, by progressive reduction of the amount of serum in the culture medium. After their adaptation, BHK-21 cells grew in suspension by forming clumps, whereas MRC-5 cells remained adherent. Then, kinetics of cell growth were studied in agitated cultures for both cell lines. In addition, kinetics of virus replication were investigated.
authors.affiliation: Viral Vaccines Research & Development Unit, Laboratory of Molecular Microbiology, Vaccinology and Biotechnology Development, Institut Pasteur de Tunis, 13, Place Pasteur, BP 74, 1002 Tunis, Tunisia.
authors.forename: Samia
authors.initials: S
authors.lastname: Rourou
authors.affiliation: Viral Vaccines Research & Development Unit, Laboratory of Molecular Microbiology, Vaccinology and Biotechnology Development, Institut Pasteur de Tunis, 13, Place Pasteur, BP 74, 1002 Tunis, Tunisia.
authors.forename: Yousr
authors.initials: Y
authors.lastname: Ben Ayed
authors.affiliation: Viral Vaccines Research & Development Unit, Laboratory of Molecular Microbiology, Vaccinology and Biotechnology Development, Institut Pasteur de Tunis, 13, Place Pasteur, BP 74, 1002 Tunis, Tunisia.
authors.forename: Khaled
authors.initials: K
authors.lastname: Trabelsi
authors.affiliation: Viral Vaccines Research & Development Unit, Laboratory of Molecular Microbiology, Vaccinology and Biotechnology Development, Institut Pasteur de Tunis, 13, Place Pasteur, BP 74, 1002 Tunis, Tunisia.
authors.forename: Samy
authors.initials: S
authors.lastname: Majoul
authors.affiliation: Viral Vaccines Research & Development Unit, Laboratory of Molecular Microbiology, Vaccinology and Biotechnology Development, Institut Pasteur de Tunis, 13, Place Pasteur, BP 74, 1002 Tunis, Tunisia. Electronic address: hela.kallel@pasteur.rns.tn.
authors.forename: Héla
authors.initials: H
authors.lastname: Kallel
mesh_terms: D000818:Animals; D019149:Bioreactors; D018929:Cell Culture Techniques; D002460:Cell Line; D002522:Chlorocebus aethiops; D006224:Cricetinae; D016895:Culture Media, Serum-Free; D006801:Humans; D011820:Rabies virus / Q000254:growth & development; D014709:Vero Cells; D014765:Viral Vaccines; D014776:Virus Cultivation
chemical_list: D016895:Culture Media, Serum-Free; D014765:Viral Vaccines
keywords: Animal component free medium; BHK-21 cells; MRC-5 cells; Measles viruses; Rabies virus; Vero cells