title: Extremely sensitive sandwich assay of kanamycin using surface-enhanced Raman scattering of 2-mercaptobenzothiazole labeled gold@silver nanoparticles.
abstract: Herein, we report the development of extremely sensitive sandwich assay of kanamycin using a combination of anti-kanamycin functionalized hybrid magnetic (Fe3O4) nanoparticles (MNPs) and 2-mercaptobenzothiazole labeled Au-core@Ag-shell nanoparticles as the recognition and surface-enhanced Raman scattering (SERS) substrate, respectively. The hybrid MNPs were first prepared via surface-mediated RAFT polymerization of N-acryloyl-L-glutamic acid in the presence of 2-(butylsulfanylcarbonylthiolsulfanyl) propionic acid-modified MNPs as a RAFT agent and then biofunctionalized with anti-kanamycin, which are both specific for kanamycin and can be collected via a simple magnet. After separating kanamycin from the sample matrix, they were sandwiched with the SERS substrate. According to our experimental results, the limit of detection (LOD) was determined to be 2pg mL(-1), this value being about 3-7 times more than sensitive than the LOD of previously reported results, which can be explained by the higher SERS activity of silver coated gold nanoparticles. The analysis time took less than 10min, including washing and optical detection steps. Furthermore, the sandwich assay was evaluated for investigating the kanamycin specificity on neomycin, gentamycin and streptomycin and detecting kanamycin in artificially contaminated milk.
authors.affiliation: Gazi University, Faculty of Science, Department of Chemistry, Besevler, 06500 Ankara, Turkey; Gazi University, Faculty of Pharmacy, Department of Analytical Chemistry, Etiler, 06330 Ankara, Turkey.
authors.forename: Adem
authors.initials: A
authors.lastname: Zengin
authors.affiliation: Gazi University, Faculty of Pharmacy, Department of Analytical Chemistry, Etiler, 06330 Ankara, Turkey.
authors.forename: Ugur
authors.initials: U
authors.lastname: Tamer
authors.affiliation: Gazi University, Faculty of Science, Department of Chemistry, Besevler, 06500 Ankara, Turkey. Electronic address: caykara@gazi.edu.tr.
authors.forename: Tuncer
authors.initials: T
authors.lastname: Caykara
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D052160:Benzothiazoles / Q000737:chemistry*; D002417:Cattle; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D052203:Ferrosoferric Oxide / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D007612:Kanamycin / Q000032:analysis* / Q000276:immunology; D058185:Magnetite Nanoparticles / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008892:Milk / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman
chemical_list: D000906:Antibodies; D052160:Benzothiazoles; D058185:Magnetite Nanoparticles; D012834:Silver; D007612:Kanamycin; C028000:dibenzothiazyl disulfide; D006046:Gold; D052203:Ferrosoferric Oxide
keywords: Kanamycin; Polymer brushes; Superparamagnetic nanoparticles; Surface enhanced Raman spectroscopy