title: Eosinophilic inflammation of chronic rhinosinusitis with nasal polyps is related to OX40 ligand expression.
abstract: The aims of this study were to investigate OX40 ligand expression in sinus tissue from patients with nasal polyposis compared with patients with chronic rhinosinusitis without nasal polyps (NPs), and to determine if OX40 ligand expression is related to eosinophilic sinus infiltration. Twenty patients with chronic rhinosinusitis (11 with and nine without NPs) and seven controls were enrolled in the study. The mRNA expression of OX40 ligand and thymic stromal lymphopoietin and its receptor were analyzed. The immunoreactivity score for OX40 ligand and the eosinophil count were obtained. The mRNA expression and immunoreactivity score of OX40 ligand were higher in patients with nasal polyposis than in patients without NPs, as well as healthy controls. The mRNA expression of thymic stromal lymphopoietin and its receptor was significantly higher in nasal polyposis than in the control, but not significantly higher than in chronic rhinosinusitis without NPs. A correlation between the number of OX40 ligand-positive cells and the number of eosinophils in sinus biopsies was found only in patients with nasal polyposis. In conclusion, the thymic stromal lymphopoietin/OX40 ligand axis is up-regulated in nasal polyposis and is related to the intensity of eosinophilic inflammation.
authors.affiliation: Medical Science Department, University of Torino, Torino, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Boita
authors.affiliation: 1st ENT Division, Clinical Physiopathology Department, University of Torino, Torino, Italy.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Garzaro
authors.affiliation: 1st ENT Division, Clinical Physiopathology Department, University of Torino, Torino, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Raimondo
authors.affiliation: 1st ENT Division, Clinical Physiopathology Department, University of Torino, Torino, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Riva
authors.affiliation: Medical Science Department, University of Torino, Torino, Italy.
authors.forename: Jasenka
authors.initials: J
authors.lastname: Mazibrada
authors.affiliation: 1st ENT Division, Clinical Physiopathology Department, University of Torino, Torino, Italy.
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Pecorari
authors.affiliation: Medical Science Department, University of Torino, Torino, Italy.
authors.forename: Caterina
authors.initials: C
authors.lastname: Bucca
authors.affiliation: Medical Science Department, University of Torino, Torino, Italy.
authors.forename: Graziella
authors.initials: G
authors.lastname: Bellone
authors.affiliation: Medical Science Department, University of Torino, Torino, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Vizio
authors.affiliation: Medical Science Department, University of Torino, Torino, Italy.
authors.forename: Enrico
authors.initials: E
authors.lastname: Heffler
authors.affiliation: Department of Clinical and Biological Sciences, Division of Respiratory Medicine, University of Torino, Torino Italy.
authors.forename: Fabio Luigi
authors.initials: FL
authors.lastname: Ricciardolo
authors.affiliation: Medical Science Department, University of Torino, Torino, Italy grolla@mauriziano.it.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Rolla
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002465:Cell Movement; D002908:Chronic Disease; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004804:Eosinophils / Q000276:immunology*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000150:complications / Q000276:immunology*; D053278:OX40 Ligand / Q000235:genetics / Q000378:metabolism*; D010256:Paranasal Sinuses / Q000378:metabolism / Q000473:pathology; D018121:Receptors, Cytokine / Q000235:genetics / Q000378:metabolism; D012220:Rhinitis / Q000150:complications / Q000276:immunology*; D012852:Sinusitis / Q000150:complications / Q000276:immunology*; D000094632:Thymic Stromal Lymphopoietin
chemical_list: C498762:CRLF2 protein, human; D016207:Cytokines; D053278:OX40 Ligand; D018121:Receptors, Cytokine; D000094632:Thymic Stromal Lymphopoietin
keywords: Chronic rhinosinusitis; OX40; TSLP; eosinophils; nasal polyps