title: Activation of spinal phosphatidylinositol 3-kinase/protein kinase B mediates pain behavior induced by plantar incision in mice.
abstract: The etiology of postoperative pain may be different from antigen-induced inflammatory pain and neuropathic pain. However, central neural plasticity plays a key role in incision pain. It is also known that phosphatidylinositol 3-kinase (PI3K) and protein kinase B/Akt (PKB/Akt) are widely expressed in laminae I-IV of the spinal horn and play a critical role in spinal central sensitization. In the present study, we explored the role of PI3K and Akt in incision pain behaviors. Plantar incision induced a time-dependent activation of spinal PI3K-p110γ and Akt, while activated Akt and PI3K-p110γ were localized in spinal neurons or microglias, but not in astrocytes. Pre-treatment with PI3K inhibitors, wortmannin or LY294002 prevented the activation of Akt brought on by plantar incision in a dose-dependent manner. In addition, inhibition of spinal PI3K signaling pathway prevented pain behaviors (dose-dependent) and spinal Fos protein expression caused by plantar incision. These data demonstrated that PI3K signaling mediated pain behaviors caused by plantar incision in mice.
authors.affiliation: Department of Neurology, Liuzhou Traditional Chinese Medical Hospital, the Third Affiliated Hospital of Guangxi University of Chinese Medicine, 32 Jiefang West Road, Liuzhou 545001, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Xu
authors.affiliation: Department of Anesthesiology, Liuzhou Traditional Chinese Medical Hospital, the Third Affiliated Hospital of Guangxi University of Chinese Medicine, 32 Jiefang West Road, Liuzhou 545001, PR China; Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Road, Wuhan 430030, PR China. Electronic address: xh_guan2013@hotmail.com.
authors.forename: Xue-Hai
authors.initials: XH
authors.lastname: Guan
authors.affiliation: Department of Anesthesiology, the Affiliated Hospital of Guilin Medical College, Guilin 543001, PR China.
authors.forename: Jun-Xiong
authors.initials: JX
authors.lastname: Yu
authors.affiliation: Department of Anesthesiology, the Affiliated Hospital of Guilin Medical College, Guilin 543001, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Lv
authors.affiliation: The First Clinical College, China Medical University, 155 Nanjing Road, Shenyang 11001, PR China.
authors.forename: Hong-Xing
authors.initials: HX
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Road, Wuhan 430030, PR China.
authors.forename: Qiao-Chu
authors.initials: QC
authors.lastname: Fu
authors.affiliation: Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Road, Wuhan 430030, PR China.
authors.forename: Hong-Bing
authors.initials: HB
authors.lastname: Xiang
authors.affiliation: Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Road, Wuhan 430030, PR China.
authors.forename: Hui-Lian
authors.initials: HL
authors.lastname: Bu
authors.affiliation: Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Road, Wuhan 430030, PR China.
authors.forename: Dai
authors.initials: D
authors.lastname: Shi
authors.affiliation: Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Road, Wuhan 430030, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Shu
authors.affiliation: Department of Anesthesiology, Liuzhou Traditional Chinese Medical Hospital, the Third Affiliated Hospital of Guangxi University of Chinese Medicine, 32 Jiefang West Road, Liuzhou 545001, PR China.
authors.forename: Li-Sheng
authors.initials: LS
authors.lastname: Qin
authors.affiliation: School of Psychology, Social Work and Human Sciences, University of West London, London, UK.
authors.forename: Anne
authors.initials: A
authors.lastname: Manyande
authors.affiliation: Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Road, Wuhan 430030, PR China. Electronic address: yktian@tjh.tjmu.edu.cn.
authors.forename: Yu-Ke
authors.initials: YK
authors.lastname: Tian
mesh_terms: D000730:Androstadienes / Q000494:pharmacology; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology*; D002867:Chromones / Q000494:pharmacology; D008297:Male; D051379:Mice; D017628:Microglia / Q000187:drug effects / Q000378:metabolism; D009025:Morpholines / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D010146:Pain / Q000378:metabolism*; D010147:Pain Measurement; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors; D000077191:Wortmannin
chemical_list: D000730:Androstadienes; D002867:Chromones; D009025:Morpholines; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D000077191:Wortmannin
keywords: Center sensitization; Fos; Incisional pain; Phosphatidylinositol 3-kinase; Protein kinase B