title: Highly immunoreactive IgG antibodies directed against a set of twenty human proteins in the sera of patients with amyotrophic lateral sclerosis identified by protein array.
abstract: Amyotrophic lateral sclerosis (ALS), the most common adult-onset motor neuron disorder, is characterized by the progressive and selective loss of upper and lower motor neurons. Diagnosis of this disorder is based on clinical assessment, and the average survival time is less than 3 years. Injections of IgG from ALS patients into mice are known to specifically mark motor neurons. Moreover, IgG has been found in upper and lower motor neurons in ALS patients. These results led us to perform a case-control study using human protein microarrays to identify the antibody profiles of serum samples from 20 ALS patients and 20 healthy controls. We demonstrated high levels of 20 IgG antibodies that distinguished the patients from the controls. These findings suggest that a panel of antibodies may serve as a potential diagnostic biomarker for ALS.
authors.affiliation: Department of Medical Proteomics/Bioanalytics, Medizinisches Proteom-Center, Ruhr-University Bochum, Bochum, Germany.
authors.forename: Caroline
authors.initials: C
authors.lastname: May
authors.affiliation: Department of Medical Proteomics/Bioanalytics, Medizinisches Proteom-Center, Ruhr-University Bochum, Bochum, Germany.
authors.forename: Eckhard
authors.initials: E
authors.lastname: Nordhoff
authors.affiliation: Institute for Prevention and Occupational Medicine of the German Social Accident Insurance, Institute of Ruhr-University Bochum, Bochum, Germany.
authors.forename: Swaantje
authors.initials: S
authors.lastname: Casjens
authors.affiliation: Department of Medical Proteomics/Bioanalytics, Medizinisches Proteom-Center, Ruhr-University Bochum, Bochum, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Turewicz
authors.affiliation: Department of Medical Proteomics/Bioanalytics, Medizinisches Proteom-Center, Ruhr-University Bochum, Bochum, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Eisenacher
authors.affiliation: St. Josef-Hospital, Ruhr-University Bochum, Bochum, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Gold
authors.affiliation: Institute for Prevention and Occupational Medicine of the German Social Accident Insurance, Institute of Ruhr-University Bochum, Bochum, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Brüning
authors.affiliation: Institute for Prevention and Occupational Medicine of the German Social Accident Insurance, Institute of Ruhr-University Bochum, Bochum, Germany.
authors.forename: Beate
authors.initials: B
authors.lastname: Pesch
authors.affiliation: Department of Medical Proteomics/Bioanalytics, Medizinisches Proteom-Center, Ruhr-University Bochum, Bochum, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Stephan
authors.affiliation: St. Josef-Hospital, Ruhr-University Bochum, Bochum, Germany.
authors.forename: Dirk
authors.initials: D
authors.lastname: Woitalla
authors.affiliation: Department of Medical Chemistry, University of Szeged, Szeged, Hungary.
authors.forename: Botond
authors.initials: B
authors.lastname: Penke
authors.affiliation: Department of Medical Chemistry, University of Szeged, Szeged, Hungary.
authors.forename: Tamás
authors.initials: T
authors.lastname: Janáky
authors.affiliation: Institute of Clinical Microbiology, University of Szeged, Szeged, Hungary.
authors.forename: Dezső
authors.initials: D
authors.lastname: Virók
authors.affiliation: Institute of Biophysics, Biological Research Center of the Hungarian Academy of Sciences, Szeged, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Siklós
authors.affiliation: Department of Neurology, University of Szeged, Szeged, Hungary.
authors.forename: Jozsef I
authors.initials: JI
authors.lastname: Engelhardt
authors.affiliation: Department of Medical Proteomics/Bioanalytics, Medizinisches Proteom-Center, Ruhr-University Bochum, Bochum, Germany ; Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V., Dortmund, Germany.
authors.forename: Helmut E
authors.initials: HE
authors.lastname: Meyer
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000690:Amyotrophic Lateral Sclerosis / Q000097:blood* / Q000175:diagnosis / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000097:blood* / Q000276:immunology*; D015415:Biomarkers / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology*; D008297:Male; D051379:Mice; D008875:Middle Aged; D009046:Motor Neurons / Q000276:immunology*; D011379:Prognosis; D040081:Protein Array Analysis; D055815:Young Adult
chemical_list: D000906:Antibodies; D015415:Biomarkers; D007074:Immunoglobulin G