title: Flow-induced regulation of brain endothelial cells in vitro.
abstract: Endothelial cell (EC) function and susceptibility to vascular disease are regulated by flow; this relationship has been modeled in systemic, but not cerebrovascular, EC culture. We studied the effects of unidirectional flow of medium, produced by orbital rotation of cultures, on morphology and protein expression in bEnd.3 mouse brain ECs. Flow altered the expression of key transcription factors and gasotransmitter-synthesizing enzymes, and increased NO production. Statins and angiotensin receptor blockers reproduced the effect of flow on endothelial nitric oxide synthase expression. Thus, flow modified brain EC properties and function in vitro, with similarities and possible differences compared to previous studies on systemic ECs. Thus, the effect of flow on brain ECs can be modeled in vitro and may assist the investigation of mechanisms of cerebrovascular disease.
authors.affiliation: Buck Institute for Research on Aging, Novato, CA, United States.
authors.forename: XiaoOu
authors.initials: X
authors.lastname: Mao
authors.affiliation: Buck Institute for Research on Aging, Novato, CA, United States.
authors.forename: Lin
authors.initials: L
authors.lastname: Xie
authors.affiliation: Buck Institute for Research on Aging, Novato, CA, United States.
authors.forename: Rose B
authors.initials: RB
authors.lastname: Greenberg
authors.affiliation: Buck Institute for Research on Aging, Novato, CA, United States.
authors.forename: Jack B
authors.initials: JB
authors.lastname: Greenberg
authors.affiliation: Buck Institute for Research on Aging, Novato, CA, United States.
authors.forename: Botao
authors.initials: B
authors.lastname: Peng
authors.affiliation: Buck Institute for Research on Aging, Novato, CA, United States.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Mieling
authors.affiliation: Buck Institute for Research on Aging, Novato, CA, United States; Department of Pharmacology and Neuroscience, University of North Texas, Fort Worth, TX, United States.
authors.forename: Kunlin
authors.initials: K
authors.lastname: Jin
authors.affiliation: Buck Institute for Research on Aging, Novato, CA, United States. Electronic address: dgreenberg@buckinstitute.org.
authors.forename: David A
authors.initials: DA
authors.lastname: Greenberg
mesh_terms: D057911:Angiotensin Receptor Antagonists / Q000494:pharmacology; D000809:Angiotensins / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D011945:Receptors, Angiotensin / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D057911:Angiotensin Receptor Antagonists; D000809:Angiotensins; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D011945:Receptors, Angiotensin; D014157:Transcription Factors; D009569:Nitric Oxide; D052250:Nitric Oxide Synthase Type III
keywords: Angiotensin receptor blocker; Atorvastatin (PubChem CID: 60823); Endothelial cell; Flow; Losartan (PubChem CID: 3961); Mevastatin (PubChem CID: 64715); Nitric oxide synthase; Simvastatin (PubChem CID: 54454); Statin; Valsartan (PubChem CID: 60846)