title: A genome-wide survey of genetic instability by transposition in Drosophila hybrids.
abstract: Hybridization between species is a genomic instability factor involved in increasing mutation rate and new chromosomal rearrangements. Evidence of a relationship between interspecific hybridization and transposable element mobilization has been reported in different organisms, but most studies are usually performed with particular TEs and do not discuss the real effect of hybridization on the whole genome. We have therefore studied whole genome instability of Drosophila interspecific hybrids, looking for the presence of new AFLP markers in hybrids. A high percentage (27-90%) of the instability markers detected corresponds to TEs belonging to classes I and II. Moreover, three transposable elements (Osvaldo, Helena and Galileo) representative of different families, showed an overall increase of transposition rate in hybrids compared to parental species. This research confirms the hypothesis that hybridization induces genomic instability by transposition bursts and suggests that genomic stress by transposition could contribute to a relaxation of mechanisms controlling TEs in the Drosophila genome.
authors.affiliation: Grup de Biología Evolutiva, Departament de Genètica i Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
authors.forename: Doris
authors.initials: D
authors.lastname: Vela
authors.affiliation: Grup de Biología Evolutiva, Departament de Genètica i Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Fontdevila
authors.affiliation: Laboratoire de Biométrie et Biologie Evolutive, UMR5558, Université Lyon1, Villeurbanne, France ; Institut Universitaire de France, Paris, France.
authors.forename: Cristina
authors.initials: C
authors.lastname: Vieira
authors.affiliation: Grup de Biología Evolutiva, Departament de Genètica i Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
authors.forename: María Pilar
authors.initials: MP
authors.lastname: García Guerreiro
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D003433:Crosses, Genetic; D017931:DNA Primers / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D004330:Drosophila / Q000235:genetics*; D042822:Genomic Instability / Q000235:genetics*; D023281:Genomics / Q000379:methods*; D006824:Hybridization, Genetic / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018709:Statistics, Nonparametric
chemical_list: D017931:DNA Primers; D004251:DNA Transposable Elements