title: A novel label-free amperometric immunosensor for carcinoembryonic antigen based on Ag nanoparticle decorated infinite coordination polymer fibres.
abstract: In this article, for the first time, a novel, high-yield and template-free method for the synthesis of Ag nanoparticle decorated thionine/infinite coordination polymer (AgNP/THI/ICP) fibres is proposed. The thionine can be adsorbed to the AgNP/THI/ICP fibres by π-conjugation and act as the redox probe. The AgNP/THI/ICP fibres not only favor the immobilization of antibody but also facilitate the electron transfer. It is found that the AgNP/THI/ICP fibres can be designed to act as a sensitive label-free electrochemical immunosensor for carcinoembryonic antigen (CEA) determination. Under the optimized conditions, the linear range of the proposed immunosensor is estimated to be from 50 fg/mL to 100 ng/mL and the detection limit is estimated to be 0.5 fg/mL at a signal-to-noise ratio of 3, respectively. The prepared immunosensor for detection of CEA shows high sensitivity, reproducibility and stability. Our study demonstrates that the proposed immunosensor has also been used to determine CEA successfully in diluted blood samples.
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Cao
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Tao
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China; School of Chemistry and Chemical Engineering, Southeast University, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Ge
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China. Electronic address: wqian@seu.edu.cn.
authors.forename: Weiping
authors.initials: W
authors.lastname: Qian
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D002272:Carcinoembryonic Antigen / Q000097:blood*; D055664:Electrochemical Techniques / Q000379:methods; D006801:Humans; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D053758:Nanoparticles / Q000737:chemistry*; D010640:Phenothiazines / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*
chemical_list: D055910:Antibodies, Immobilized; D002272:Carcinoembryonic Antigen; D010640:Phenothiazines; D012834:Silver; C009469:thionine
keywords: Carcinoembryonic antigen; Fibres; Immunosensor; Infinite coordination polymer; Thionine