title: In situ molecular imaging of a hydrated biofilm in a microfluidic reactor by ToF-SIMS.
abstract: A novel microfluidic reactor for biofilm growth and in situ characterization using time-of-flight secondary ion mass spectrometry (ToF-SIMS) was constructed to enable two-dimensional chemical imaging of hydrated biofilms. We demonstrate the detection of characteristic fatty acid fragments from microfluidic reactor-grown biofilms and illustrate advantages of hydrated-state ToF-SIMS imaging.
authors.affiliation: Fundamental and Computer Sciences, Pacific Northwest National Laboratory (PNNL), Richland, WA 99352, USA. xiaoying.yu@pnnl.gov.
authors.forename: Xin
authors.initials: X
authors.lastname: Hua
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Yu
authors.forename: Zhaoying
authors.initials: Z
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Bingwen
authors.initials: B
authors.lastname: Liu
authors.forename: Zihua
authors.initials: Z
authors.lastname: Zhu
authors.forename: Abigail E
authors.initials: AE
authors.lastname: Tucker
authors.forename: William B
authors.initials: WB
authors.lastname: Chrisler
authors.forename: Eric A
authors.initials: EA
authors.lastname: Hill
authors.forename: Theva
authors.initials: T
authors.lastname: Thevuthasan
authors.forename: Yuehe
authors.initials: Y
authors.lastname: Lin
authors.forename: Songqin
authors.initials: S
authors.lastname: Liu
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Marshall
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D004867:Equipment Design; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D057054:Molecular Imaging / Q000295:instrumentation / Q000379:methods*; D018629:Spectrometry, Mass, Secondary Ion / Q000295:instrumentation / Q000379:methods*; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water