title: In vitro culture conditions and OeARF and OeH3 expressions modulate adventitious root formation from oleaster (Olea europaea L. subsp. europaea var. sylvestris) cuttings.
abstract: Olea europaea L. subsp. europaea var. sylvestris, also named oleaster, is the wild form of olive and it is used as rootstock and pollen donor for many cultivated varieties. An efficient procedure for in vitro propagation of oleaster was established in this study. A zeatin concentration of 2.5 mg/L was effective to induce an appreciable vegetative growth. Also high rooting efficiency was obtained by using a short IBA pulse, followed by two different IBA concentrations in the culture medium. With the aim to enlarge knowledge on the molecular aspects of adventitious rooting, we also evaluated the transcriptional modulation of an ARFs member and HISTONE H3 genes, involved in auxin signaling and cell replication, respectively, during the root induction phase of cuttings. The obtained results suggest that the selected genes, as markers of the induction phase, could be very useful for setting up efficient culture conditions along the rooting process, thus increasing micropropagation efficiency.
authors.affiliation: Department DiBEST, University of Calabria, Ponte P. Bucci, Arcavacata di Rende, 87036 Cosenza, Italy.
authors.forename: Adriana
authors.identifier: 0000-0001-9725-9905
authors.initials: A
authors.lastname: Chiappetta
authors.affiliation: Department DiBEST, University of Calabria, Ponte P. Bucci, Arcavacata di Rende, 87036 Cosenza, Italy.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Gagliardi
authors.affiliation: Department DiBEST, University of Calabria, Ponte P. Bucci, Arcavacata di Rende, 87036 Cosenza, Italy.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Bruno
authors.affiliation: Department DiBEST, University of Calabria, Ponte P. Bucci, Arcavacata di Rende, 87036 Cosenza, Italy.
authors.forename: Maria Beatrice
authors.initials: MB
authors.lastname: Bitonti
mesh_terms: D000704:Analysis of Variance; D002455:Cell Division / Q000502:physiology; D017931:DNA Primers / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D015723:Gene Library; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D031658:Olea / Q000254:growth & development* / Q000378:metabolism*; D018517:Plant Roots / Q000254:growth & development*; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics; D046509:Tissue Culture Techniques / Q000379:methods*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D006657:Histones; D014157:Transcription Factors