title: CXCL5 knockdown expression inhibits human bladder cancer T24 cells proliferation and migration.
abstract: CXCL5 (epithelial neutrophil activating peptide-78) which acts as a potent chemoattractant and activator of neutrophil function was reported to play a multifaceted role in tumorigenesis. To investigate the role of CXCL5 in bladder cancer progression, we examined the CXCL5 expression in bladder cancer tissues by real-time PCR and Western blot, additionally, we used shRNA-mediated silencing to generate stable CXCL5 silenced bladder cancer T24 cells and defined its biological functions. Our results demonstrated that mRNA and protein of CXCL5 is increased in human bladder tumor tissues and cell lines, down-regulation of CXCL5 in T24 cells resulted in significantly decreased cell proliferation, migration and increased cell apoptosis in vitro through Snail, PI3K-AKT and ERK1/2 signaling pathways. These data suggest that CXCL5 is critical for bladder tumor growth and progression, it may represent a potential application in cancer diagnosis and therapy.
authors.affiliation: Department of Laboratory Medicine, Peking University Third Hospital, Beijing, China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Department of Urology, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Laboratory Medicine, Peking University Third Hospital, Beijing, China. Electronic address: zhangjiebjmu@163.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D014408:Biomarkers, Tumor / Q000235:genetics / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation; D054365:Chemokine CXCL5 / Q000037:antagonists & inhibitors* / Q000235:genetics* / Q000502:physiology; D055785:Gene Knockdown Techniques; D006801:Humans; D020935:MAP Kinase Signaling System; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy*
chemical_list: D014408:Biomarkers, Tumor; C486263:CXCL5 protein, human; D054365:Chemokine CXCL5; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; D019869:Phosphatidylinositol 3-Kinases
keywords: Bladder cancer; CXCL5; Cells proliferation and migration; T24 cells