title: Antioxidant and antiangiogenic activity of Astronium graveolens Jacq. leaves.
abstract: Angiogenesis is involved in many physiological and pathological conditions. Natural compounds with antioxidant activity have also been reported to possess potent antiangiogenic properties by regulating angiogenesis modulators such as vascular endothelial growth factor (VEGF). Based on this, we screened the antioxidant and antiangiogenic activities of Astronium graveolens leaf extracts by a DPPH test and a competitive enzyme-linked immunosorbent assay, respectively. MeOH extract expressed a significant free radical-scavenging activity (EC₅₀ = 37.65 μg/mL) and it was able to inhibit the interaction between placental growth factor (PlGF) (placental growth factor), a VEGF family member, and its receptor Flt-1 by more than 50% at 1 mg/mL. 1,2,3,4,6-Penta-O-galloyl-d-glucopyranose, 6 is the most active compound of the extract. It exhibited a high potency in scavenging DPPH (EC₅₀ = 2.16 μg/mL) and reduced by 58% the PlGF/Flt-1 interaction at a concentration of 50 μM. Moreover, the known compounds (1-6) have been isolated for the first time in A. graveolens.
authors.affiliation: a Department of Pharmacognosy and Organic Medicaments , University of Los Andes , Mérida 5101 , Venezuela.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Hernández
authors.forename: Nicola
authors.initials: N
authors.lastname: Malafronte
authors.forename: Flor
authors.initials: F
authors.lastname: Mora
authors.forename: Maria S
authors.initials: MS
authors.lastname: Pesca
authors.forename: Rita P
authors.initials: RP
authors.lastname: Aquino
authors.forename: Teresa
authors.initials: T
authors.lastname: Mencherini
mesh_terms: D027926:Anacardiaceae / Q000737:chemistry*; D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D047348:Hydrolyzable Tannins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000072483:Placenta Growth Factor; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D011257:Pregnancy Proteins / Q000187:drug effects; D042461:Vascular Endothelial Growth Factor A / Q000187:drug effects; D040281:Vascular Endothelial Growth Factor Receptor-1
chemical_list: D020533:Angiogenesis Inhibitors; D000975:Antioxidants; D047348:Hydrolyzable Tannins; D010936:Plant Extracts; D011257:Pregnancy Proteins; D042461:Vascular Endothelial Growth Factor A; D000072483:Placenta Growth Factor; C435084:pentagalloylglucose; D040281:Vascular Endothelial Growth Factor Receptor-1
keywords: 1,2,3,4,6-penta-O-galloyl-d-glucopyranose; Astronium graveolens; antioxidant and antiangiogenic activity; placental growth factor