title: PrP expression level and sensitivity to prion infection.
abstract: Mice overexpressing the prion protein (PrP) sequence from various host species are widely used for measuring infectious titers in prion disease. However, the impact that the transgene expression level might have on the susceptibility to infection raises some concerns about the final biological relevance of these models. Here we report that endpoint titration of a sheep scrapie isolate in sheep and in mice overexpressing the ovine PrP results in similar estimates of the infectious titer.
authors.affiliation: INRA, UMR 1225, Interactions Hôtes Agents Pathogènes, Ecole Nationale Vétérinaire de Toulouse, Toulouse, France.
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Douet
authors.forename: Caroline
authors.initials: C
authors.lastname: Lacroux
authors.forename: Fabien
authors.initials: F
authors.lastname: Corbière
authors.forename: Claire
authors.initials: C
authors.lastname: Litaise
authors.forename: Hugh
authors.initials: H
authors.lastname: Simmons
authors.forename: Séverine
authors.initials: S
authors.lastname: Lugan
authors.forename: Pierrette
authors.initials: P
authors.lastname: Costes
authors.forename: Hervé
authors.initials: H
authors.lastname: Cassard
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Weisbecker
authors.forename: François
authors.initials: F
authors.lastname: Schelcher
authors.forename: Olivier
authors.initials: O
authors.lastname: Andreoletti
mesh_terms: D000818:Animals; D004198:Disease Susceptibility; D015870:Gene Expression; D051379:Mice; D018620:PrPSc Proteins / Q000096:biosynthesis*; D017096:Prion Diseases / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D012756:Sheep
chemical_list: D018620:PrPSc Proteins; D011994:Recombinant Proteins