title: [Study on the effect of deficient ERCC2/XPD gene on the repair of DNA damage induced by UVC in CHO cell line].
abstract: OBJECTIVE
  To explore the function of ERCC2/XPD in the repair of DNA damage induced by UVC.
  
  
  METHODS
  Chinese hamster ovary (CHO) cell line including AA8 (wild-type) and UV5 (mutant type, ERCC2/XPD defective), was selected as a cell control model. The cell inhibition rate of AA8 and UV5 after UVC treatment was estimated by MTT assay, and DNA repair capacity to difference irradiation intensity of UVC in cells after 1 h, 3 h, 6 h, 24 h incubation were measured by the Comet Assay and Rad51 immunofluorescence test.
  
  
  RESULTS
  As compared to AA8, UV5 was more sensitive to UVC, and whose cell viability decreased. Comet assay and Rad51 immunofluorescence test results show, DNA damage level of UV5 was more serious than AA8. In addition, the DNA damage repair capacity reduced obviously compared with AA8.
  
  
  CONCLUSIONS
  DNA damage repair capacity of UV5 cells reduced due to ERCC2/XPD defective, indicating us that ERCC2/XPD play a critical role in the repair process of DNA damage induced by UVC.
authors.affiliation: Hygiene Toxicology Department, Public Health School, China Medical University, Shenyang 110001, China. gyy_87@163.com
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Guan
authors.affiliation: Hygiene Toxicology Department, Public Health School, China Medical University, Shenyang 110001, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Xiao
authors.affiliation: Hygiene Toxicology Department, Public Health School, China Medical University, Shenyang 110001, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Li
authors.affiliation: Hygiene Toxicology Department, Public Health School, China Medical University, Shenyang 110001, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Xue
authors.affiliation: Hygiene Toxicology Department, Public Health School, China Medical University, Shenyang 110001, China.
authors.forename: Guopei
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Hygiene Toxicology Department, Public Health School, China Medical University, Shenyang 110001, China.
authors.forename: Qiufang
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Hygiene Toxicology Department, Public Health School, China Medical University, Shenyang 110001, China.
authors.forename: Cuihong
authors.initials: C
authors.lastname: Jin
authors.affiliation: Hygiene Toxicology Department, Public Health School, China Medical University, Shenyang 110001, China.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yang
authors.affiliation: Hygiene Toxicology Department, Public Health School, China Medical University, Shenyang 110001, China.
authors.forename: Shengwen
authors.initials: S
authors.lastname: Wu
authors.affiliation: Hygiene Toxicology Department, Public Health School, China Medical University, Shenyang 110001, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Lu
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage / Q000528:radiation effects*; D004260:DNA Repair / Q000235:genetics*; D009154:Mutation; D014466:Ultraviolet Rays; D051759:Xeroderma Pigmentosum Group D Protein / Q000235:genetics*
chemical_list: D051759:Xeroderma Pigmentosum Group D Protein