title: Covalent TiO(2)/pectin microspheres with Fe(3)O(4) nanoparticles for magnetic field-modulated drug delivery.
abstract: Covalent TiO(2)-co-pectin microspheres containing Fe(3)O(4) nanoparticles were developed through an ultrasound-induced crosslinking/polymerization reaction between the glycidyl methacrylate from vinyl groups in TiO(2) and in pectin. ζ-potentials became less negative in the nanostructured microspheres, caused by the presence of both inorganic particles in the negatively charged pectin. The nanostructured pectin microspheres showed an amoxicillin release rate slower than that of pure pectin microspheres. The proposed microspheres were found to be a sustained release system of amoxicillin in the acid medium. Furthermore, the antibiotic release may be modulated by exposition of the microspheres to a remote magnetic field. In practical terms, the nanostructured microspheres could deliver a larger proportion of their initial load to specific site of action. The cytotoxic concentrations for 50% of VERO cells (CC(50)), calculated as the concentration required to reduce cell viability by 50% after 72h of incubation, for pectin-only microspheres and nanostructured pectin microspheres were 217.7±6.5 and 121.5±4.9μgmL(-1), respectively. The obtained CC(50) values indicated acceptable cytotoxic levels for an incubation period of 72h, showing that the pectin microspheres have a great pharmacological potential for uses in biological environments, even after the introduction of both Fe(3)O(4) and TiO(2).
authors.affiliation: Department of Chemistry, State University of Maringá, Av. Colombo, 5790, CEP 87020-900 Maringá, Paraná, Brazil.
authors.forename: Elisangela P
authors.initials: EP
authors.lastname: da Silva
authors.affiliation: Department of Chemistry, State University of Maringá, Av. Colombo, 5790, CEP 87020-900 Maringá, Paraná, Brazil.
authors.forename: Danielly L A
authors.initials: DL
authors.lastname: Sitta
authors.affiliation: Department of Chemistry, State University of Maringá, Av. Colombo, 5790, CEP 87020-900 Maringá, Paraná, Brazil.
authors.forename: Vanessa H
authors.initials: VH
authors.lastname: Fragal
authors.affiliation: Department of Chemistry, State University of Maringá, Av. Colombo, 5790, CEP 87020-900 Maringá, Paraná, Brazil.
authors.forename: Thelma S P
authors.initials: TS
authors.lastname: Cellet
authors.affiliation: Department of Chemistry, State University of Maringá, Av. Colombo, 5790, CEP 87020-900 Maringá, Paraná, Brazil.
authors.forename: Marcos R
authors.initials: MR
authors.lastname: Mauricio
authors.affiliation: Department of Basic Sciences of Health, State University of Maringá, Av. Colombo, 5790, CEP 87020-900 Maringá, Paraná, Brazil.
authors.forename: Francielle P
authors.initials: FP
authors.lastname: Garcia
authors.affiliation: Department of Basic Sciences of Health, State University of Maringá, Av. Colombo, 5790, CEP 87020-900 Maringá, Paraná, Brazil; Graduate Program in Pharmaceutical Sciences, Department of Basic Science of Health, State University of Maringá, Av. Colombo, 5790, CEP 87020-900 Maringá, Paraná, Brazil.
authors.forename: Celso V
authors.initials: CV
authors.lastname: Nakamura
authors.affiliation: Department of Chemistry, State University of Maringá, Av. Colombo, 5790, CEP 87020-900 Maringá, Paraná, Brazil.
authors.forename: Marcos R
authors.initials: MR
authors.lastname: Guilherme
authors.affiliation: Department of Chemistry, State University of Maringá, Av. Colombo, 5790, CEP 87020-900 Maringá, Paraná, Brazil.
authors.forename: Adley F
authors.initials: AF
authors.lastname: Rubira
authors.affiliation: Department of Chemistry, State University of Maringá, Av. Colombo, 5790, CEP 87020-900 Maringá, Paraná, Brazil. Electronic address: mhkunita@gmail.com.
authors.forename: Marcos H
authors.initials: MH
authors.lastname: Kunita
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D016503:Drug Delivery Systems; D005290:Ferric Compounds / Q000737:chemistry / Q000627:therapeutic use; D006801:Humans; D060526:Magnetic Fields; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use; D008863:Microspheres; D010368:Pectins / Q000737:chemistry* / Q000627:therapeutic use; D017550:Spectroscopy, Fourier Transform Infrared; D014025:Titanium / Q000737:chemistry* / Q000627:therapeutic use; D014709:Vero Cells
chemical_list: D005290:Ferric Compounds; C009495:titanium dioxide; C000499:ferric oxide; D010368:Pectins; D014025:Titanium
keywords: Drug delivery; Nanotechnology; Pectin