title: Evaluation of hbetaD-1 and hbetaD-2 levels in saliva of patients with oral mucosal diseases.
abstract: OBJECTIVE
  This study aimed to determine a possible correlation between oral mucosal disease and salivary concentrations of the antimicrobial peptides human beta-defensin-1 (hbetaD-1) and human beta-defensin-2 (hbetaD-2).
  
  
  METHODS
  The present work focussed on the establishment of a reversed phase-high performance liquid chromatography (RP-HPLC) procedure to quantify human beta-defensins (hbetaD-1 and hbetaD-2) in saliva samples of patients with oral diseases such as lichen planus (n = 10), Behçet (n = 10) and recurrent apthous stomatitis (n = 10).
  
  
  RESULTS
  Linear calibration range for hbetaD-1 and hbetaD-2 defensins was 1.67-200 microg mL-1 and 3.13 -100 PG mL-1 with R2 values of 0.9998 and 0.996, correspondingly. The concentration of beta-defensins in saliva was determined by comparing the peak areas of eluted hbetaD-1 and hbetaD-2 with that of their standards. The variation of the amount of beta-defensins was evaluated by comparisons of the results obtained from the patients with oral mucosal diseases before and after treatments and the control subjects. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 1.62 microg mL-Sand 5.39 microg mL-1 for hbetaD-1 and 0.94 mig mL-1 and 3.13 microg mL-1 for hbetalD-2, respectively.
  
  
  CONCLUSIONS
  The salivary beta-defensin concentration was significantly higher in patients with oral mucosal diseases than in healthy volunteers; furthermore, in patients with oral mucosal diseases, the concentration was significantly higher before treatment than after treatment.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Selcuk University, Konya, Turkey. ksemahat@gmail.com
authors.forename: H
authors.initials: H
authors.lastname: Kucukkolbasi
authors.affiliation: Department of Chemistry, Faculty of Science, Selcuk University, Konya, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Kucukkolbasi
authors.affiliation: Department of Chemistry, Faculty of Science, Selcuk University, Konya, Turkey.
authors.forename: H F
authors.initials: HF
authors.lastname: Ayyildiz
authors.affiliation: Department of Dermatology, Medical Faculty, Baskent University, Konya, Turkey.
authors.forename: R
authors.initials: R
authors.lastname: Dursun
authors.affiliation: Department of Chemistry, Faculty of Science, Selcuk University, Konya, Turkey.
authors.forename: H
authors.initials: H
authors.lastname: Kara
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001528:Behcet Syndrome / Q000378:metabolism* / Q000628:therapy; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008010:Lichen Planus / Q000378:metabolism* / Q000628:therapy; D008297:Male; D008875:Middle Aged; D009059:Mouth Diseases / Q000378:metabolism / Q000628:therapy; D009061:Mouth Mucosa; D012463:Saliva / Q000737:chemistry*; D013281:Stomatitis, Aphthous / Q000378:metabolism* / Q000628:therapy; D055815:Young Adult; D023083:beta-Defensins / Q000378:metabolism*
chemical_list: C406407:DEFB1 protein, human; C106641:DEFB4A protein, human; D023083:beta-Defensins