title: Statistical tests to identify appropriate types of nucleotide sequence recoding in molecular phylogenetics.
abstract: BACKGROUND
  Under a Markov model of evolution, recoding, or lumping, of the four nucleotides into fewer groups may permit analysis under simpler conditions but may unfortunately yield misleading results unless the evolutionary process of the recoded groups remains Markovian. If a Markov process is lumpable, then the evolutionary process of the recoded groups is Markovian.
  
  
  RESULTS
  We consider stationary, reversible, and homogeneous Markov processes on two taxa and compare three tests for lumpability: one using an ad hoc test statistic, which is based on an index that is evaluated using a bootstrap approximation of its distribution; one that is based on a test proposed specifically for Markov chains; and one using a likelihood-ratio test. We show that the likelihood-ratio test is more powerful than the index test, which is more powerful than that based on the Markov chain test statistic. We also show that for stationary processes on binary trees with more than two taxa, the tests can be applied to all pairs. Finally, we show that if the process is lumpable, then estimates obtained under the recoded model agree with estimates obtained under the original model, whereas, if the process is not lumpable, then these estimates can differ substantially. We apply the new likelihood-ratio test for lumpability to two primate data sets, one with a mitochondrial origin and one with a nuclear origin.
  
  
  CONCLUSIONS
  Recoding may result in biased phylogenetic estimates because the original evolutionary process is not lumpable. Accordingly, testing for lumpability should be done prior to phylogenetic analysis of recoded data.
authors.forename: Victor A
authors.initials: VA
authors.lastname: Vera-Ruiz
authors.forename: Kwok W
authors.initials: KW
authors.lastname: Lau
authors.forename: John
authors.initials: J
authors.lastname: Robinson
authors.forename: Lars S
authors.initials: LS
authors.lastname: Jermiin
mesh_terms: D000818:Animals; D004272:DNA, Mitochondrial / Q000737:chemistry; D016013:Likelihood Functions; D008390:Markov Chains; D009711:Nucleotides / Q000032:analysis; D010802:Phylogeny; D011323:Primates; D017422:Sequence Analysis, DNA / Q000379:methods*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004272:DNA, Mitochondrial; D009711:Nucleotides