title: On the dual effect of glucose during production of pBAD/AraC-based minicircles.
abstract: Minicircles are promising vectors for DNA vaccination, gene or cell therapies due to their increased transfection efficacy and transgene expression. The in vivo production of these novel vectors involves the arabinose inducible excision of a parental molecule into a minicircircle and a miniplasmid bacterial backbone. Tight control of recombination is crucial to maximize minicircle yields and purity. In this work, a minicircle production system was constructed that relies on the enzymatic activity of ParA resolvase, a recombinase that is expressed under the transcription control of the arabinose inducible expression system pBAD/AraC, and on Escherichia coli BWAA, a strain improved for arabinose uptake. Undesired recombination already after 4h of incubation in Luria-Bertani broth at 37 °C was observed due to the leaky expression from pBAD/AraC. While addition of glucose to the growth media repressed this leaky expression, it triggered a pH drop to 4.5 during exponential phase in shake flasks, which suppressed growth and plasmid production. The quantitative PCR analysis confirmed only few copies of high-copy number plasmid inside of the E. coli cells. To ensure the stability of minicircle-producing system, seed cultures should be grown at 30 °C with glucose overnight whereas cells for minicircle production should be grown in shake flasks at 37 °C without glucose up to early stationary phase when the recombination is induced by addition of arabinose.
authors.affiliation: MIT-Portugal Program, Portugal; Department of Bioengineering, Instituto Superior Técnico (IST), Lisbon, Portugal; IBB-Institute for Biotechnology and Bioengineering, Center for Biological and Chemical Engineering, IST, Lisbon, Portugal.
authors.forename: Michaela
authors.initials: M
authors.lastname: Simcikova
authors.affiliation: MIT-Portugal Program, Portugal; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, United States.
authors.forename: Kristala L J
authors.initials: KL
authors.lastname: Prather
authors.affiliation: MIT-Portugal Program, Portugal; Department of Bioengineering, Instituto Superior Técnico (IST), Lisbon, Portugal; IBB-Institute for Biotechnology and Bioengineering, Center for Biological and Chemical Engineering, IST, Lisbon, Portugal.
authors.forename: Duarte M F
authors.initials: DM
authors.lastname: Prazeres
authors.affiliation: MIT-Portugal Program, Portugal; Department of Bioengineering, Instituto Superior Técnico (IST), Lisbon, Portugal; IBB-Institute for Biotechnology and Bioengineering, Center for Biological and Chemical Engineering, IST, Lisbon, Portugal. Electronic address: gabmonteiro@ist.utl.pt.
authors.forename: Gabriel A
authors.initials: GA
authors.lastname: Monteiro
mesh_terms: D001089:Arabinose / Q000737:chemistry; D003470:Culture Media / Q000737:chemistry; D003561:Cytarabine / Q000378:metabolism; D004270:DNA, Circular / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D005947:Glucose / Q000737:chemistry*; D010957:Plasmids; D045522:Recombinases / Q000378:metabolism; D011995:Recombination, Genetic
chemical_list: D003470:Culture Media; D004270:DNA, Circular; D045522:Recombinases; D003561:Cytarabine; D001089:Arabinose; D005947:Glucose
keywords: DNA vaccine; Glucose; Minicircle; Recombination; l-(+)arabinose