title: Antioxidative activity of protein hydrolysate produced by alcalase hydrolysis from shrimp waste (Penaeus monodon and Penaeus indicus).
abstract: Protein hydrolysates prepared by hydrolysis of shrimp waste (Penaeus monodon and Penaeus indicus) for 90 min. using Alcalase enzyme following pH-stat method. Antioxidative activities of SWPH were assessed determining FRAP, ABTS and DPPH radical scavenging activities, which increased linearly with increasing concentration of protein hydrolysate upto 5 mg/ml maintaining good correlation. SWPH showed high stability over wide ranges of pH (2-11) and temperature (up to 100 °C for 150 min), in which the activity of more than 80% was retained. Protein hydrolysate solution with a concentration of 5 mg/ml significantly lowered TBA values of Croaker fish fillet and maintained yellowishness of skin colour compared to untreated control sample during 10 days of refrigerated storage at 4 °C. SWPH also restricted the increase of PV and FFA values in Croaker fish fillet within acceptable limit.
authors.affiliation: Department of Fish Processing Technology, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences 5, Budherhat Road P.O: Panchasayar, Kolkata, 700 094 West Bengal India.
authors.forename: Satya Sadhan
authors.initials: SS
authors.lastname: Dey
authors.affiliation: Department of Fish Processing Technology, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences 5, Budherhat Road P.O: Panchasayar, Kolkata, 700 094 West Bengal India.
authors.forename: Krushna Chandra
authors.initials: KC
authors.lastname: Dora
keywords: Antioxidative activity; Protein hydrolysate; Shrimp waste