title: Antiproliferative effect of Canavalia brasiliensis lectin on B16F10 cells.
abstract: Lectins are proteins or glycoproteins with the ability to link to carbohydrates at the cell surface in a specific and reversible manner. Studies have showed that lectins are demonstrate immunomodulatory and antitumor agents. This study aims to evaluate the effect of lectin extracted from the seeds of Canavalia brasiliensis (ConBr) on murine melanoma B16F10 cells by analyzing cell viability, apoptosis index, cell migration, production of cytokines and nitric oxide (NO). Results showed that ConBr was able to reduce cell viability and thwart apoptosis, which could be observed by decrease in cell migration. ConBr also induced NO and IL-12 synthesis. Altogether, these data demonstrate the potential of ConBr as a therapeutic agent for melanoma.
authors.affiliation: Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Pernambuco, Brazil. Electronic address: foliveirasilva@gmail.com.
authors.forename: Flávio de Oliveira
authors.initials: Fde O
authors.lastname: Silva
authors.affiliation: Centro de Ciências biológicas, Universidade Federal de Pernambuco, Pernambuco, Brazil.
authors.forename: Priscila das Neves
authors.initials: Pd
authors.lastname: Santos
authors.affiliation: Departamento de Imunologia do Centro de Pesquisas Aggeu Magalhães - CPqAM/FIOCRUZ-UFPE, Pernambuco, Brazil.
authors.forename: Evellyne de Oliveira
authors.initials: Ede O
authors.lastname: Figueirôa
authors.affiliation: Departamento de Imunologia do Centro de Pesquisas Aggeu Magalhães - CPqAM/FIOCRUZ-UFPE, Pernambuco, Brazil.
authors.forename: Cristiane Moutinho Lagos
authors.initials: CM
authors.lastname: de Melo
authors.affiliation: Departamento de Imunologia do Centro de Pesquisas Aggeu Magalhães - CPqAM/FIOCRUZ-UFPE, Pernambuco, Brazil.
authors.forename: Juliana Kelle
authors.initials: JK
authors.lastname: de Andrade Lemoine Neves
authors.affiliation: Biomol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Brazil.
authors.forename: Francisco Vassiliepe Sousa
authors.initials: FV
authors.lastname: Arruda
authors.affiliation: Biomol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Brazil.
authors.forename: João Batista
authors.initials: JB
authors.lastname: Cajazeiras
authors.affiliation: Biomol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Brazil.
authors.forename: Kyria Santiago
authors.initials: KS
authors.lastname: do Nascimento
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Brazil.
authors.forename: Edson Holanda
authors.initials: EH
authors.lastname: Teixeira
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Brazil.
authors.forename: Benildo Sousa
authors.initials: BS
authors.lastname: Cavada
authors.affiliation: Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Pernambuco, Brazil. Electronic address: analuporto@yahoo.com.br.
authors.forename: Ana Lúcia Figueiredo
authors.initials: AL
authors.lastname: Porto
authors.affiliation: Departamento de Imunologia do Centro de Pesquisas Aggeu Magalhães - CPqAM/FIOCRUZ-UFPE, Pernambuco, Brazil.
authors.forename: Valéria Rêgo Alves
authors.initials: VR
authors.lastname: Pereira
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D032449:Canavalia / Q000276:immunology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000276:immunology; D002470:Cell Survival / Q000276:immunology; D016207:Cytokines / Q000032:analysis; D005434:Flow Cytometry; D008545:Melanoma / Q000188:drug therapy* / Q000276:immunology; D051379:Mice; D009569:Nitric Oxide / Q000032:analysis; D037121:Plant Lectins / Q000494:pharmacology* / Q000627:therapeutic use; D018709:Statistics, Nonparametric
chemical_list: D016207:Cytokines; D037121:Plant Lectins; C039268:lectin, Canavalia; D009569:Nitric Oxide
keywords: Antiproliferative activity; Canavalia brasiliensis; ConBr; Glucose/manose binding lectin; Murine melanoma