title: Antibiotic drug tigecycline inhibited cell proliferation and induced autophagy in gastric cancer cells.
abstract: Tigecycline acts as a glycylcycline class bacteriostatic agent, and actively resists a series of bacteria, specifically drug fast bacteria. However, accumulating evidence showed that tetracycline and their derivatives such as doxycycline and minocycline have anti-cancer properties, which are out of their broader antimicrobial activity. We found that tigecycline dramatically inhibited gastric cancer cell proliferation and provided an evidence that tigecycline induced autophagy but not apoptosis in human gastric cancer cells. Further experiments demonstrated that AMPK pathway was activated accompanied with the suppression of its downstream targets including mTOR and p70S6K, and ultimately induced cell autophagy and inhibited cell growth. So our data suggested that tigecycline might act as a candidate agent for pre-clinical evaluation in treatment of patients suffering from gastric cancer.
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, PR China.
authors.forename: Chunling
authors.initials: C
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, PR China.
authors.forename: Liqun
authors.initials: L
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, PR China.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Division of Scientific Research and Training, General Hospital of PLA Chengdu Military Area Command, Chengdu, Sichuan 610083, PR China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, PR China.
authors.forename: Mei
authors.initials: M
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, PR China.
authors.forename: Qinrui
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Institute of Urinary Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, PR China. Electronic address: zhouzhans@sina.com.
authors.forename: Zhansong
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, PR China.
authors.forename: Zhonghuai
authors.initials: Z
authors.lastname: Xiang
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, PR China. Electronic address: hcui@swu.edu.cn.
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Cui
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001343:Autophagy / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D008911:Minocycline / Q000031:analogs & derivatives* / Q000494:pharmacology; D038762:Ribosomal Protein S6 Kinases, 70-kDa / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism; D000078304:Tigecycline; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D000078304:Tigecycline; C546842:MTOR protein, human; D038762:Ribosomal Protein S6 Kinases, 70-kDa; D058570:TOR Serine-Threonine Kinases; D055372:AMP-Activated Protein Kinases; D008911:Minocycline
keywords: Autophagy; Gastric cancer; Proliferation inhibition; Tigecycline