title: Assessment of Mycobacterium bovis deleted in p27-p55 virulence operon as candidate vaccine against tuberculosis in animal models.
abstract: A Mycobacterium bovis knockout in p27-p55 operon was tested as an antituberculosis experimental vaccine in animal models. The mutant MbΔp27-p55 was significantly more attenuated in nude mice than its parental strain but more virulent than BCG Pasteur. Challenge experiments in mice and guinea pigs using M. bovis or M. tuberculosis strains showed similar protection conferred by MbΔp27-p55 mutant than BCG in terms of pathology and bacterial loads in spleen but lower protection than BCG in lungs. When tested in cattle, MbΔp27-p55 did not induce IL-2 expression and induced a very low production of IFNγ, suggesting that the lack of P27/P55 reduces the capacity of M. bovis of triggering an adequate Th1 response.
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, N. Repetto and De los Reseros, 1686 Hurlingham, Argentina.
authors.forename: María V
authors.initials: MV
authors.lastname: Bianco
authors.affiliation: Public Health England, Porton Down, Salisbury SP4 0JG, UK.
authors.forename: Simon
authors.initials: S
authors.lastname: Clark
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, N. Repetto and De los Reseros, 1686 Hurlingham, Argentina.
authors.forename: Federico C
authors.initials: FC
authors.lastname: Blanco
authors.affiliation: Instituto de Patobiología, CICVyA-INTA, N. Repetto and De los Reseros, 1686 Hurlingham, Argentina.
authors.forename: Sergio
authors.initials: S
authors.lastname: Garbaccio
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, N. Repetto and De los Reseros, 1686 Hurlingham, Argentina.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: García
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, N. Repetto and De los Reseros, 1686 Hurlingham, Argentina.
authors.forename: Angel A
authors.initials: AA
authors.lastname: Cataldi
authors.affiliation: Public Health England, Porton Down, Salisbury SP4 0JG, UK.
authors.forename: Ann
authors.identifier: 0000-0002-9494-0372
authors.initials: A
authors.lastname: Williams
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, N. Repetto and De los Reseros, 1686 Hurlingham, Argentina.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Bigi
mesh_terms: D000818:Animals; D001500:BCG Vaccine / Q000627:therapeutic use; D001426:Bacterial Proteins / Q000235:genetics*; D002417:Cattle; D006168:Guinea Pigs; D006801:Humans; D007376:Interleukin-2 / Q000276:immunology / Q000378:metabolism; D008074:Lipoproteins / Q000235:genetics*; D026901:Membrane Transport Proteins / Q000235:genetics*; D051379:Mice; D023421:Models, Animal; D009163:Mycobacterium bovis / Q000235:genetics* / Q000472:pathogenicity; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000472:pathogenicity; D009876:Operon / Q000235:genetics / Q000276:immunology; D014376:Tuberculosis / Q000235:genetics / Q000276:immunology* / Q000517:prevention & control
chemical_list: D001500:BCG Vaccine; D001426:Bacterial Proteins; D007376:Interleukin-2; D008074:Lipoproteins; D026901:Membrane Transport Proteins; C110121:P27 protein, Mycobacterium; C425143:P55 protein, Mycobacterium