title: Global small-angle X-ray scattering data analysis for multilamellar vesicles: the evolution of the scattering density profile model.
abstract: The highly successful scattering density profile (SDP) model, used to jointly analyze small-angle X-ray and neutron scattering data from unilamellar vesicles, has been adapted for use with data from fully hydrated, liquid crystalline multilamellar vesicles (MLVs). Using a genetic algorithm, this new method is capable of providing high-resolution structural information, as well as determining bilayer elastic bending fluctuations from standalone X-ray data. Structural parameters such as bilayer thickness and area per lipid were determined for a series of saturated and unsaturated lipids, as well as binary mixtures with cholesterol. The results are in good agreement with previously reported SDP data, which used both neutron and X-ray data. The inclusion of deuterated and non-deuterated MLV neutron data in the analysis improved the lipid backbone information but did not improve, within experimental error, the structural data regarding bilayer thickness and area per lipid.
authors.affiliation: Instiute of Molecular Biosciences, Biophysics Division, University of Graz, Austria.
authors.forename: Peter
authors.initials: P
authors.lastname: Heftberger
authors.affiliation: Instiute of Molecular Biosciences, Biophysics Division, University of Graz, Austria.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Kollmitzer
authors.affiliation: Biology and Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.
authors.forename: Frederick A
authors.initials: FA
authors.lastname: Heberle
authors.affiliation: Biology and Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA ; Department of Physics, University of South Florida, Tampa, FL 33620, USA.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Pan
authors.affiliation: Institute of Inorganic Chemistry, Graz University of Technology, Austria ; School of Food Science and Nutrition, University of Leeds, UK.
authors.forename: Michael
authors.initials: M
authors.lastname: Rappolt
authors.affiliation: Institute of Inorganic Chemistry, Graz University of Technology, Austria.
authors.forename: Heinz
authors.initials: H
authors.lastname: Amenitsch
authors.affiliation: Canadian Neutron Beam Centre, National Research Council, Chalk River, ON, Canada.
authors.forename: Norbert
authors.initials: N
authors.lastname: Kučerka
authors.affiliation: Biology and Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA ; Joint Institute for Neutron Sciences, Oak Ridge, TN, USA ; Department of Physics and Astronomy, University of Tennessee, Knoxville, TN, USA ; Department of Physics, Brock University, St Catharines, ON, Canada.
authors.forename: John
authors.initials: J
authors.lastname: Katsaras
authors.affiliation: Instiute of Molecular Biosciences, Biophysics Division, University of Graz, Austria.
authors.forename: Georg
authors.initials: G
authors.lastname: Pabst
keywords: genetic algorithms; liquid crystalline multilamellar vesicles; scattering density profile model; small-angle X-ray scattering