title: Muscovy duck reovirus p10.8 protein localizes to the nucleus via a nonconventional nuclear localization signal.
abstract: BACKGROUND
  It was previously report that the first open reading frame of Muscovy duck reocvirus S4 gene encodes a 95-amino-acid protein, designed p10.8, which has no sequence similarity to other known proteins. Its amino acid sequence offers no clues about its function.
  
  
  RESULTS
  Subcellular localization and nuclear import signal of p10.8 were characterized. We found that p10.8 protein localizes to the nucleus of infected and transfected cells, suggesting that p10.8 nuclear localization is not facilitated by viral infection or any other viral protein. A functional non-canonical nuclear localization signal (NLS) for p10.8 was identified and mapped to N-terminus residues 1-40. The NLS has the ability to retarget a large cytoplasmic protein to the nucleus.
  
  
  CONCLUSIONS
  p10.8 imported into the nucleus might via a nonconventional signal nuclear signal.
authors.forename: Dongchun
authors.initials: D
authors.lastname: Guo
authors.forename: Na
authors.initials: N
authors.lastname: Qiu
authors.forename: Wulin
authors.initials: W
authors.lastname: Shaozhou
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Bai
authors.forename: Yilong
authors.initials: Y
authors.lastname: He
authors.forename: Qingshan
authors.initials: Q
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, P R China. liuming04@126.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D046090:Anseriformes; D002467:Cell Nucleus / Q000378:metabolism*; D004372:Ducks; D019913:Nuclear Localization Signals; D030101:Orthoreovirus, Avian / Q000235:genetics / Q000502:physiology*; D021381:Protein Transport; D012087:Reoviridae; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D019913:Nuclear Localization Signals; D014764:Viral Proteins