title: Changes in bacterial population of gastrointestinal tract of weaned pigs fed with different additives.
abstract: This study aimed to provide novel insights into the gastrointestinal microbial diversity from different gastrointestinal locations in weaning piglets using PCR-restriction fragment length polymorphism (PCR-RFLP). Additionally, the effect of different feed additives was analyzed. Thirty-two piglets were fed with four different diets: a control group and three enriched diets, with avilamycin, sodium butyrate, and a plant extract mixture. Digesta samples were collected from eight different gastrointestinal segments of each animal and the bacterial population was analysed by a PCR-RFLP technique that uses 16S rDNA gene sequences. Bacterial diversity was assessed by calculating the number of bands and the Shannon-Weaver index. Dendrograms were constructed to estimate the similarity of bacterial populations. A higher bacterial diversity was detected in large intestine compared to small intestine. Among diets, the most relevant microbial diversity differences were found between sodium butyrate and plant extract mixture. Proximal jejunum, ileum, and proximal colon were identified as those segments that could be representative of microbial diversity in pig gut. Results indicate that PCR-RFLP technique allowed detecting modifications on the gastrointestinal microbial ecology in pigs fed with different additives, such as increased biodiversity by sodium butyrate in feed.
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain.
authors.forename: Mercè
authors.initials: M
authors.lastname: Roca
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain.
authors.forename: Miquel
authors.initials: M
authors.lastname: Nofrarías
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain ; Departament de Sanitat i Anatomia Animals, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain.
authors.forename: Natàlia
authors.initials: N
authors.lastname: Majó
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain ; Institut de Recerca i Tecnologia Agroalimentaria (IRTA), Caldes de Montbui, 08140 Barcelona, Spain.
authors.forename: Ana María
authors.initials: AM
authors.lastname: Pérez de Rozas
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain ; Departament de Sanitat i Anatomia Animals, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain.
authors.forename: Joaquim
authors.identifier: 0000-0002-1539-7261
authors.initials: J
authors.lastname: Segalés
authors.affiliation: Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain.
authors.forename: Marisol
authors.initials: M
authors.lastname: Castillo
authors.affiliation: Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain.
authors.forename: Susana María
authors.initials: SM
authors.lastname: Martín-Orúe
authors.affiliation: Servei d'Estadística (SEA), Edifici D, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: Espinal
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain ; Institut de Recerca i Tecnologia Agroalimentaria (IRTA), Caldes de Montbui, 08140 Barcelona, Spain.
authors.forename: Joan
authors.initials: J
authors.lastname: Pujols
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain ; Institut de Recerca i Tecnologia Agroalimentaria (IRTA), Caldes de Montbui, 08140 Barcelona, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Badiola
mesh_terms: D000818:Animals; D001419:Bacteria / Q000187:drug effects; D044822:Biodiversity; D015169:Colony Count, Microbial; D004032:Diet; D005247:Feeding Behavior / Q000187:drug effects*; D005503:Food Additives / Q000494:pharmacology*; D041981:Gastrointestinal Tract / Q000187:drug effects / Q000382:microbiology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D034421:Sus scrofa; D014886:Weaning
chemical_list: D005503:Food Additives