title: The brain-enriched microRNA miR-124 in plasma predicts neurological outcome after cardiac arrest.
abstract: BACKGROUND
  Early prognostication after successful cardiopulmonary resuscitation is difficult, and there is a need for novel methods to estimate the extent of brain injury and predict outcome. In this study, we evaluated the impact of the cardiac arrest syndrome on the plasma levels of selected tissue-specific microRNAs (miRNAs) and assessed their ability to prognosticate death and neurological disability.
  
  
  METHODS
  We included 65 patients treated with hypothermia after cardiac arrest in the study. Blood samples were obtained at 24 hours and at 48 hours. For miRNA-screening purposes, custom quantitative polymerase chain reaction (qPCR) panels were first used. Thereafter individual miRNAs were assessed at 48 hours with qPCR. miRNAs that successfully predicted prognosis at 48 hours were further analysed at 24 hours. Outcomes were measured according to the Cerebral Performance Category (CPC) score at 6 months after cardiac arrest and stratified into good (CPC score 1 or 2) or poor (CPC scores 3 to 5).
  
  
  RESULTS
  At 48 hours, miR-146a, miR-122, miR-208b, miR-21, miR-9 and miR-128 did not differ between the good and poor neurological outcome groups. In contrast, miR-124 was significantly elevated in patients with poor outcomes compared with those with favourable outcomes (P < 0.0001) at 24 hours and 48 hours after cardiac arrest. Analysis of receiver operating characteristic curves at 24 and 48 hours after cardiac arrest showed areas under the curve of 0.87 (95% confidence interval (CI) = 0.79 to 0.96) and 0.89 (95% CI = 0.80 to 0.97), respectively.
  
  
  CONCLUSIONS
  The brain-enriched miRNA miR-124 is a promising novel biomarker for prediction of neurological prognosis following cardiac arrest.
authors.forename: Patrik
authors.initials: P
authors.lastname: Gilje
authors.forename: Olof
authors.initials: O
authors.lastname: Gidlöf
authors.forename: Malin
authors.initials: M
authors.lastname: Rundgren
authors.forename: Tobias
authors.initials: T
authors.lastname: Cronberg
authors.forename: Mariam
authors.initials: M
authors.lastname: Al-Mashat
authors.forename: Björn
authors.initials: B
authors.lastname: Olde
authors.forename: Hans
authors.initials: H
authors.lastname: Friberg
authors.forename: David
authors.initials: D
authors.lastname: Erlinge
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D001921:Brain / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D005500:Follow-Up Studies; D006323:Heart Arrest / Q000097:blood* / Q000175:diagnosis* / Q000628:therapy; D006801:Humans; D007036:Hypothermia, Induced / Q000639:trends; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D011237:Predictive Value of Tests; D012189:Retrospective Studies; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D015415:Biomarkers; C509207:MIRN124 microRNA, human; D035683:MicroRNAs