title: Preparation and in vitro evaluation of glycyrrhetinic acid-modified curcumin-loaded nanostructured lipid carriers.
abstract: Curcumin, a phenolic antioxidant compound derived from the rhizome of the turmeric plant Curcuma longa, has proven to be a modulator of intracellular signaling pathways that control cancer cell growth, inflammation, invasion and apoptosis, revealing its anticancer potential. In this study, a Glycyrrhetinic Acid-Modified Curcumin-Loaded Nanostructured Lipid Carrier (Cur-GA-PEG-NLC) was prepared by the film ultrasound method to improve the tumor-targeting ability. The drug content was detected by an UV spectrophotometry method. The encapsulation efficiency of curcumin in the nanostructured lipid carriers (NLCs) was determined using a mini-column centrifugation method. The encapsulation efficiency for various Cur-GA-PEG-NLC was within the range of 90.06%-95.31% and particle size was between 123.1 nm and 132.7 nm. An in vitro MTT assay showed that Cur-GA10%-PEG-NLC had significantly high cellular uptake and cytotoxicity against HepG2 cells compared with other groups.
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital of China Medical University, 155 Nanjing Street, Shenyang 110001, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Chu
authors.affiliation: Department of Pharmacy, Central Hospital Attached to Shenyang Medical College, No. 5 the 7th south-west Road, TieXi district, Shenyang 110024, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital of China Medical University, 155 Nanjing Street, Shenyang 110001, China.
authors.forename: Yi-Fan
authors.initials: YF
authors.lastname: Luo
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Street, Shenyang 110016, China.
authors.forename: Xiao-Jin
authors.initials: XJ
authors.lastname: He
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital of China Medical University, 155 Nanjing Street, Shenyang 110001, China. ydyyyxb@163.com.
authors.forename: Ming-Yan
authors.initials: MY
authors.lastname: Jiang
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects*; D003474:Curcumin / Q000737:chemistry / Q000494:pharmacology*; D004337:Drug Carriers; D004355:Drug Stability; D006034:Glycyrrhetinic Acid / Q000737:chemistry / Q000494:pharmacology; D056945:Hep G2 Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry; D010316:Particle Size
chemical_list: D004337:Drug Carriers; D008055:Lipids; D003474:Curcumin; D006034:Glycyrrhetinic Acid