title: CMOS-compatible silicon nanowire field-effect transistors for ultrasensitive and label-free microRNAs sensing.
abstract: MicroRNAs (miRNAs) have been regarded as promising biomarkers for the diagnosis and prognosis of early-stage cancer as their expression levels are associated with different types of human cancers. However, it is a challenge to produce low-cost miRNA sensors, as well as retain a high sensitivity, both of which are essential factors that must be considered in fabricating nanoscale biosensors and in future biomedical applications. To address such challenges, we develop a complementary metal oxide semiconductor (CMOS)-compatible SiNW-FET biosensor fabricated by an anisotropic wet etching technology with self-limitation which provides a much lower manufacturing cost and an ultrahigh sensitivity. This nanosensor shows a rapid (< 1 minute) detection of miR-21 and miR-205, with a low limit of detection (LOD) of 1 zeptomole (ca. 600 copies), as well as an excellent discrimination for single-nucleotide mismatched sequences of tumor-associated miRNAs. To investigate its applicability in real settings, we have detected miRNAs in total RNA extracted from lung cancer cells as well as human serum samples using the nanosensors, which demonstrates their potential use in identifying clinical samples for early diagnosis of cancer.
authors.affiliation: State Key Laboratories of Transducer, Technology & Science and Technology on Micro-system Laboratory, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, 200050, Shanghai, China.
authors.forename: Na
authors.initials: N
authors.lastname: Lu
authors.forename: Anran
authors.initials: A
authors.lastname: Gao
authors.forename: Pengfei
authors.initials: P
authors.lastname: Dai
authors.forename: Shiping
authors.initials: S
authors.lastname: Song
authors.forename: Chunhai
authors.initials: C
authors.lastname: Fan
authors.forename: Yuelin
authors.initials: Y
authors.lastname: Wang
authors.forename: Tie
authors.initials: T
authors.lastname: Li
mesh_terms: D020137:Base Pair Mismatch / Q000235:genetics; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation; D004553:Electric Conductivity; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000235:genetics; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics*; D008969:Molecular Sequence Data; D053770:Nanowires / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017423:Sequence Analysis, RNA / Q000379:methods; D012825:Silicon / Q000737:chemistry*; D013194:Staining and Labeling; D014173:Transistors, Electronic; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; D012825:Silicon
keywords: field-effect transistors (FETs); label-free; microRNA; nanowires; sensors; ultrasensitive