title: Upconversion fluorescent strip sensor for rapid determination of Vibrio anguillarum.
abstract: Here, we report a simple and ultrasensitive upconversion fluorescent strip sensor based on NaYF4:Yb,Er nanoparticles (NPs) and the lateral flow immunochromatographic assay (LFIA). Carboxyl-modified β-NaYF4:Yb,Er NPs were successfully synthesized by a facile one-pot solvothermal approach, upon further coupling with monoclonal antibody, the resultant UCNPs-antibody conjugates probes were used in LFIA and served as signal vehicles for the fluorescent reporters. V. anguillarum was used as a model analyte to demonstrate the use of this strip sensor. The limit of the detection for the fluorescent strip was determined as 10(2) CFU mL(-1), which is 100 times lower than those displayed by enzyme-linked immunosorbent assays, while the time needed for the detection was only 15 min. Furthermore, no cross-reaction with other eight pathogens was found, indicating the good specificity of the strip. This developed LFIA would offer the potential as a useful tool for the quantification of pathogens analysis in the future.
authors.affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. yhzhu@ecust.edu.cn czli@ecust.edu.cn.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wu
authors.forename: Yihua
authors.initials: Y
authors.lastname: Zhu
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Yang
authors.forename: Xin
authors.initials: X
authors.lastname: Jiang
authors.forename: Jingfan
authors.initials: J
authors.lastname: Xiao
authors.forename: Yuanxing
authors.initials: Y
authors.lastname: Zhang
authors.forename: Chunzhong
authors.initials: C
authors.lastname: Li
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D000941:Antigens / Q000032:analysis / Q000276:immunology; D002846:Chromatography, Affinity; D003429:Cross Reactions; D004871:Erbium / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D005459:Fluorides / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D014733:Vibrio / Q000302:isolation & purification* / Q000378:metabolism; D015018:Ytterbium / Q000737:chemistry; D015019:Yttrium / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D005456:Fluorescent Dyes; C525362:sodium yttriumtetrafluoride; D015019:Yttrium; D004871:Erbium; D015018:Ytterbium; D005459:Fluorides