title: Bacillus licheniformis BT5.9 Isolated from Changar Hot Spring, Malang, Indonesia, as a Potential Producer of Thermostable α-amylase.
abstract: A total of 34 bacterial isolates were obtained from soil samples collected from Changar Hot Spring, Malang, Indonesia. Of these, 13 isolates produced a zone of hydrolysis in starch-nutrient agar medium and generated various amylases in liquid medium. One isolate was selected as the best amylase producer and was identified as Bacillus licheniformis BT5.9. The improvement of culture conditions (initial medium pH of 5.0, cultivation temperature of 50°C, agitation speed of 100 rpm and inoculum size of 1.7 × 10(9) cells/ml) provided the highest amylase production (0.327 U/ml).
authors.affiliation: Industrial Biotechnology Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia.
authors.forename: Darah
authors.initials: D
authors.lastname: Ibrahim
authors.affiliation: Industrial Biotechnology Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia.
authors.forename: Han Li
authors.initials: HL
authors.lastname: Zhu
authors.affiliation: Industrial Biotechnology Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia.
authors.forename: Nuraqilah
authors.initials: N
authors.lastname: Yusof
authors.affiliation: Faculty of Pharmacy, Universitas Air Langga, Surabaya, Indonesia.
authors.lastname: Isnaeni
authors.affiliation: Industrial Biotechnology Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia.
authors.forename: Lim Sheh
authors.initials: LS
authors.lastname: Hong
keywords: Amylase; Bacillus licheniformis; Hot Spring; Thermophilic Bacteria