title: Production of polyhydroxyalkanoates from spent coffee grounds oil obtained by supercritical fluid extraction technology.
abstract: Spent coffee grounds (SCG) oil was obtained by supercritical carbon dioxide (scCO2) extraction in a pilot plant apparatus, with an oil extraction yield of 90% at a 35kgkg(-1) CO2/SCG ratio. Cupriavidus necator DSM 428 was cultivated in 2L bioreactor using extracted SCG oil as sole carbon source for production of polyhydroxyalkanoates. The culture reached a cell dry weight of 16.7gL(-1) with a polymer content of 78.4% (w/w). The volumetric polymer productivity and oil yield were 4.7gL(-1)day(-1) and 0.77gg(-1), respectively. The polymer produced was a homopolymer of 3-hydroxybutyrate with an average molecular weight of 2.34×10(5) and a polydispersity index of 1.2. The polymer exhibited brittle behaviour, with very low elongation at break (1.3%), tensile strength at break of 16MPa and Young's Modulus of 1.0GPa. Results show that SCG can be a bioresource for polyhydroxyalkanoates production with interesting properties.
authors.affiliation: REQUIMTE/CQFB, Chemistry Department, FCT/Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Madalena V
authors.initials: MV
authors.lastname: Cruz
authors.affiliation: REQUIMTE/CQFB, Chemistry Department, FCT/Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Paiva
authors.affiliation: REQUIMTE/CQFB, Chemistry Department, FCT/Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Pedro
authors.initials: P
authors.lastname: Lisboa
authors.affiliation: REQUIMTE/CQFB, Chemistry Department, FCT/Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Filomena
authors.initials: F
authors.lastname: Freitas
authors.affiliation: CEER - Biosystems Engineering Center, Institute of Agronomy, University of Lisbon, 1349-017 Lisboa, Portugal.
authors.forename: Vítor D
authors.initials: VD
authors.lastname: Alves
authors.affiliation: REQUIMTE/CQFB, Chemistry Department, FCT/Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Pedro
authors.initials: P
authors.lastname: Simões
authors.affiliation: REQUIMTE/CQFB, Chemistry Department, FCT/Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Susana
authors.initials: S
authors.lastname: Barreiros
authors.affiliation: REQUIMTE/CQFB, Chemistry Department, FCT/Universidade Nova de Lisboa, 2829-516 Caparica, Portugal. Electronic address: amr@fct.unl.pt.
authors.forename: Maria A M
authors.initials: MA
authors.lastname: Reis
mesh_terms: D018533:Biomass; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D003069:Coffee / Q000737:chemistry*; D020118:Cupriavidus necator / Q000254:growth & development / Q000378:metabolism*; D009821:Oils / Q000737:chemistry*; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*; D014866:Waste Products
chemical_list: D003069:Coffee; D009821:Oils; D054813:Polyhydroxyalkanoates; D014866:Waste Products
keywords: Cupriavidus necator; Polyhydroxyalkanoates (PHAs); Spent coffee grounds (SCG); Supercritical fluid extraction (SFE); Triglycerides