title: Engineering Synechocystis PCC6803 for hydrogen production: influence on the tolerance to oxidative and sugar stresses.
abstract: In the prospect of engineering cyanobacteria for the biological photoproduction of hydrogen, we have studied the hydrogen production machine in the model unicellular strain Synechocystis PCC6803 through gene deletion, and overexpression (constitutive or controlled by the growth temperature). We demonstrate that the hydrogenase-encoding hoxEFUYH operon is dispensable to standard photoautotrophic growth in absence of stress, and it operates in cell defense against oxidative (H₂O₂) and sugar (glucose and glycerol) stresses. Furthermore, we showed that the simultaneous over-production of the proteins HoxEFUYH and HypABCDE (assembly of hydrogenase), combined to an increase in nickel availability, led to an approximately 20-fold increase in the level of active hydrogenase. These novel results and mutants have major implications for those interested in hydrogenase, hydrogen production and redox metabolism, and their connections with environmental conditions.
authors.affiliation: UMR8221, CEA, CNRS, Université Paris Sud, Institut de Biologie et Technologie Saclay, Laboratoire de Biologie et Biotechnologie des Cyanobactéries, CEA-Saclay, Gif sur Yvette, France.
authors.forename: Marcia
authors.initials: M
authors.lastname: Ortega-Ramos
authors.affiliation: UMR8221, CEA, CNRS, Université Paris Sud, Institut de Biologie et Technologie Saclay, Laboratoire de Biologie et Biotechnologie des Cyanobactéries, CEA-Saclay, Gif sur Yvette, France.
authors.forename: Thichakorn
authors.initials: T
authors.lastname: Jittawuttipoka
authors.affiliation: UMR8221, CEA, CNRS, Université Paris Sud, Institut de Biologie et Technologie Saclay, Laboratoire de Biologie et Biotechnologie des Cyanobactéries, CEA-Saclay, Gif sur Yvette, France.
authors.forename: Panatda
authors.initials: P
authors.lastname: Saenkham
authors.affiliation: UMR8221, CEA, CNRS, Université Paris Sud, Institut de Biologie et Technologie Saclay, Laboratoire de Biologie et Biotechnologie des Cyanobactéries, CEA-Saclay, Gif sur Yvette, France.
authors.forename: Aurelia
authors.initials: A
authors.lastname: Czarnecka-Kwasiborski
authors.affiliation: UMR8221, CEA, CNRS, Université Paris Sud, Institut de Biologie et Technologie Saclay, Laboratoire des Mécanismes fondamentaux de la Bioénergétique, CEA-Saclay, Gif sur Yvette, France.
authors.forename: Hervé
authors.initials: H
authors.lastname: Bottin
authors.affiliation: UMR8221, CEA, CNRS, Université Paris Sud, Institut de Biologie et Technologie Saclay, Laboratoire de Biologie et Biotechnologie des Cyanobactéries, CEA-Saclay, Gif sur Yvette, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Cassier-Chauvat
authors.affiliation: UMR8221, CEA, CNRS, Université Paris Sud, Institut de Biologie et Technologie Saclay, Laboratoire de Biologie et Biotechnologie des Cyanobactéries, CEA-Saclay, Gif sur Yvette, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Chauvat
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D006864:Hydrogenase / Q000378:metabolism; D009876:Operon / Q000235:genetics; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000235:genetics / Q000502:physiology*; D046939:Synechocystis / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D006859:Hydrogen; D006861:Hydrogen Peroxide; D006864:Hydrogenase; D005947:Glucose; D005990:Glycerol