title: Enzymatic electrosynthesis of formate through CO2 sequestration/reduction in a bioelectrochemical system (BES).
abstract: Bioelectrochemical system (BES) was operated using the enzyme formate dehydrogenase as catalyst at cathode in its free form for the reduction of CO2 into formic acid. Electrosynthesis of formic acid was higher at an operational voltage of -1V vs. Ag/AgCl (9.37mgL(-1) CO2) compared to operation at -0.8V (4.73mgL(-1) CO2) which was strongly supported by the reduction catalytic current. Voltammograms also depicted a reversible redox peak throughout operation at -1V, indicating NAD(+) recycling for proton transfer from the source to CO2. Saturation of the product was observed after 45min of enzyme addition and then reversibility commenced, depicting a lower and stable formic acid concentration throughout the subsequent time of operation. Stability of the enzyme activity after immobilization on the electrode and product yield will be studied further.
authors.affiliation: Separation and Conversion Technologies, VITO - Flemish Institute for Technological Research, Boeretang 200, Mol 2400, Belgium.
authors.forename: Sandipam
authors.initials: S
authors.lastname: Srikanth
authors.affiliation: Separation and Conversion Technologies, VITO - Flemish Institute for Technological Research, Boeretang 200, Mol 2400, Belgium.
authors.forename: Miranda
authors.initials: M
authors.lastname: Maesen
authors.affiliation: Separation and Conversion Technologies, VITO - Flemish Institute for Technological Research, Boeretang 200, Mol 2400, Belgium.
authors.forename: Xochitl
authors.initials: X
authors.lastname: Dominguez-Benetton
authors.affiliation: Separation and Conversion Technologies, VITO - Flemish Institute for Technological Research, Boeretang 200, Mol 2400, Belgium.
authors.forename: Karolien
authors.initials: K
authors.lastname: Vanbroekhoven
authors.affiliation: Separation and Conversion Technologies, VITO - Flemish Institute for Technological Research, Boeretang 200, Mol 2400, Belgium. Electronic address: deepak.pant@vito.be.
authors.forename: Deepak
authors.initials: D
authors.lastname: Pant
mesh_terms: D001674:Bioelectric Energy Sources; D019149:Bioreactors; D002245:Carbon Dioxide / Q000378:metabolism*; D002384:Catalysis; D055664:Electrochemical Techniques / Q000379:methods*; D005560:Formate Dehydrogenases / Q000378:metabolism*; D005561:Formates / Q000378:metabolism*; D013997:Time Factors
chemical_list: D005561:Formates; C030544:formic acid; D002245:Carbon Dioxide; D005560:Formate Dehydrogenases
keywords: Bioelectrochemical system (BES); CO(2) reduction; Enzymatic electrosynthesis; Formate dehydrogenase (FDH); Formic acid