title: Express immunochromatographic detection of antibodies against Brucella abortus in cattle sera based on quantitative photometric registration and modulated cut-off level.
abstract: An immunochromatographic test system was developed for rapid detection of the levels of specific IgG antibodies to Brucella abortus lipopolysaccharide, as a tool for diagnosis of brucellosis in cattle. The pilot test strips were examined using blood sera from sick (78 samples) and healthy (35 samples) cows. The results obtained by immunochromatographic assay, using a portable optical densitometer for digital video detection, correlate well with the results obtained by immunoenzyme assay and are in agreement with the results of the disease diagnosis. The new test system allows detection of antibodies within 10 min and can be proposed as an alternative to the methods available for serodiagnosis of brucellosis.
authors.affiliation: a A.N. Bach Institute of Biochemistry, Russian Academy of Sciences , Moscow , Russia.
authors.forename: Dmitriy V
authors.initials: DV
authors.lastname: Sotnikov
authors.forename: Nadezhda A
authors.initials: NA
authors.lastname: Byzova
authors.forename: Anatoly V
authors.initials: AV
authors.lastname: Zherdev
authors.forename: Saule Z
authors.initials: SZ
authors.lastname: Eskendirova
authors.forename: Kairat K
authors.initials: KK
authors.lastname: Baltin
authors.forename: Kasim K
authors.initials: KK
authors.lastname: Mukanov
authors.forename: Erlan M
authors.initials: EM
authors.lastname: Ramankulov
authors.forename: Elchin G
authors.initials: EG
authors.lastname: Sadykhov
authors.forename: Boris B
authors.initials: BB
authors.lastname: Dzantiev
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002003:Brucella abortus / Q000166:cytology / Q000254:growth & development / Q000276:immunology*; D002417:Cattle; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D008070:Lipopolysaccharides / Q000276:immunology; D010783:Photometry
chemical_list: D000906:Antibodies; D008070:Lipopolysaccharides
keywords: brucellosis; diagnostics; immunochromatographic assay; rapid test