title: PHBV/PAM scaffolds with local oriented structure through UV polymerization for tissue engineering.
abstract: Locally oriented tissue engineering scaffolds can provoke cellular orientation and direct cell spread and migration, offering an exciting potential way for the regeneration of the complex tissue. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) scaffolds with locally oriented hydrophilic polyacrylamide (PAM) inside the macropores of the scaffolds were achieved through UV graft polymerization. The interpenetrating PAM chains enabled good interconnectivity of PHBV/PAM scaffolds that presented a lower porosity and minor diameter of pores than PHBV scaffolds. The pores with diameter below 100  μm increased to 82.15% of PHBV/PAM scaffolds compared with 31.5% of PHBV scaffolds. PHBV/PAM scaffold showed a much higher compressive elastic modulus than PHBV scaffold due to PAM stuffing. At 5 days of culturing, sheep chondrocytes spread along the similar direction in the macropores of PHBV/PAM scaffolds. The locally oriented PAM chains might guide the attachment and spreading of chondrocytes and direct the formation of microfilaments via contact guidance.
authors.affiliation: Department of Biomedical Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China ; Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University, Guangzhou 510632, China.
authors.forename: Yu
authors.identifier: 0000-0002-8176-0014
authors.initials: Y
authors.lastname: Ke
authors.affiliation: Biomaterial Research Institute, School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China.
authors.forename: Gang
authors.identifier: 0000-0002-4730-4971
authors.initials: G
authors.lastname: Wu
authors.affiliation: National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
authors.forename: Yingjun
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure; D019245:Compressive Strength / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology*; D058105:Polymerization / Q000187:drug effects / Q000528:radiation effects*; D016062:Porosity; D012756:Sheep; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014466:Ultraviolet Rays; D000099166:Polyacrylamides; D000094122:Polyhydroxybutyrates
chemical_list: D000180:Acrylic Resins; D011091:Polyesters; D000099166:Polyacrylamides; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates