title: Evaluation of the oxidative deoxyribonucleic acid damage biomarker 8-hydroxy-2'-deoxyguanosine in the urine of leukemic children by micellar electrokinetic capillary chromatography.
abstract: Determining the level of urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG), an oxidative DNA damage biomarker, is vital to the study of clinical pathogenesis and drug toxicity. The principal limitation of capillary electrophoresis (CE) with UV detection is its low sensitivity. To overcome this shortcoming, we developed a micellar electrokinetic capillary chromatography (MEKC) with solid-phase extraction (SPE) for urinary 8-OHdG analysis. The sensitivity of MEKC-UV was improved using a reasonable UV system, injection mode, and SPE. The parameters affecting MEKC and SPE were also optimized. The calibration curve was linear within the range from 1 to 500 μg L(-1). The limits of detection and quantification were 0.27 μg L(-1) and 0.82 μg L(-1), respectively. Interday and intraday precision were both <5.6%. The recovery of 8-OHdG in urine ranged from 94.5% to 103.2%. This method was used to measure urinary 8-OHdG from eight normal children, eight newly diagnosed leukemic children, and eight leukemic children undergoing chemotherapy. The results show that the proposed method can be used to assess oxidative stress in patients and the side effects of chemotherapeutic drugs by measuring urinary 8-OHdG.
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang, PR China.
authors.forename: Pingping
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Sanitary Inspection, School of Public Health, Hebei Medical University, Shijiazhuang, PR China.
authors.forename: Kaoqi
authors.initials: K
authors.lastname: Lian
authors.affiliation: Department of Pediatrics, the Second Hospital of Hebei Medical University, Shijiazhuang, PR China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Yao
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Sanitary Inspection, School of Public Health, Hebei Medical University, Shijiazhuang, PR China.
authors.forename: Weijun
authors.initials: W
authors.lastname: Kang
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang, PR China. Electronic address: guiyang1959@126.com.
authors.forename: Lingling
authors.initials: L
authors.lastname: Jiang
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D015415:Biomarkers / Q000652:urine; D002648:Child; D002675:Child, Preschool; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000652:urine; D005260:Female; D006801:Humans; D007938:Leukemia / Q000652:urine*; D008297:Male; D018384:Oxidative Stress; D052616:Solid Phase Extraction / Q000379:methods
chemical_list: D015415:Biomarkers; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine
keywords: 8-Hydroxy-2′-deoxyguanosine; Chemotherapy; Leukemia; Micellar electrokinetic capillary chromatography