title: A false positive case due to matrix interference in the analysis of ronidazole residues in muscle tissue using LC-MS/MS.
abstract: In contrast with the information of the inspection body concerning the use of ronidazole, several non compliant muscle samples were detected using a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method in accordance with confirmation criteria of Decision 2002/657/EC. This led to the suspicion that non compliance could be due to false positive results. In this context, a liquid chromatography-high resolution mass spectrometry (LC-HRMS) method was developed and sample extracts were re-analyzed, resolving the co eluting isobaric interfering peak, which also has an interfering product ion with the transition product (m/z 201>140).
authors.affiliation: Departament de Química Analítica, Universitat de Barcelona, Barcelona, Spain.
authors.forename: Praveen
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Laboratori Agència Salut Pública de Barcelona, Barcelona, Spain; CIBER of Epidemiology and Public Health (CIBERESP), Madrid, Spain.
authors.forename: Antoni
authors.initials: A
authors.lastname: Rúbies
authors.affiliation: Laboratori Agència Salut Pública de Barcelona, Barcelona, Spain; CIBER of Epidemiology and Public Health (CIBERESP), Madrid, Spain.
authors.forename: Francesc
authors.initials: F
authors.lastname: Centrich
authors.affiliation: Departament de Química Analítica, Universitat de Barcelona, Barcelona, Spain. Electronic address: compano@ub.edu.
authors.forename: Ramon
authors.initials: R
authors.lastname: Companyó
mesh_terms: D000890:Anti-Infective Agents / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D006801:Humans; D007477:Ions; D008460:Meat / Q000032:analysis*; D009132:Muscles / Q000737:chemistry*; D012385:Ronidazole / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000890:Anti-Infective Agents; D007477:Ions; D012385:Ronidazole
keywords: False positive case; High resolution mass spectrometry; Muscle; Quadrupole–Orbitrap; Ronidazole