title: Cord blood cytokine levels in focal early-onset neonatal infection after preterm premature rupture of membranes.
abstract: This study aimed to evaluate the levels of pro- and anti-inflammatory cytokines in umbilical cord blood of preterm neonates who developed focal early-onset infection (EOI) after preterm premature rupture of membranes (PPROM). This is a prospective study conducted on 46 preterm infants from mothers with PPROM. The cytokines were measure by flow cytometry. Newborns were classified into two groups as focal EOI (n=19) and non-infected (n=27). Interleukin (IL)-6 and IL-8 levels were higher, whereas IL-10 and IL-12 p70 levels were lower in the EOI when compared to the non-infected group. The best combination of cytokines was IL-6+IL-8, with a diagnostic accuracy of 0.97. Focal EOI after PPROM is associated with increased levels of IL-6 and IL-8 and diminished IL-10 and IL-12 in the cord blood of preterm infants. Combined assessment of IL-6 and IL-8 in cord blood may provide an additional tool for identifying preterm infants who develop EOI after PPROM.
authors.affiliation: Department of Pediatrics Bioscience Institute of Botucatu, UNESP, Botucatu, São Paulo, Brazil. kurokawa@fmb.unesp.br, cskurokawa@gmail.com.
authors.forename: Cilmery Suemi
authors.initials: CS
authors.lastname: Kurokawa
authors.forename: Miriam
authors.initials: M
authors.lastname: Hashimoto
authors.forename: Ligia Maria Suppo
authors.initials: LM
authors.lastname: de Souza Rugolo
authors.forename: Maria Regina
authors.initials: MR
authors.lastname: Bentlin
authors.forename: Marjorie de Assis
authors.initials: Mde A
authors.lastname: Golin
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Peraçoli
authors.forename: Marcia Guimarães
authors.initials: MG
authors.lastname: da Silva
authors.forename: José Eduardo
authors.initials: JE
authors.lastname: Corrente
authors.forename: Saskia Maria Wiegerinck
authors.initials: SM
authors.lastname: Fekete
mesh_terms: D015415:Biomarkers / Q000097:blood; D016207:Cytokines / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005312:Fetal Blood / Q000378:metabolism*; D005322:Premature Rupture of Fetal Membranes / Q000097:blood* / Q000276:immunology; D005434:Flow Cytometry; D005500:Follow-Up Studies; D006801:Humans; D007113:Immunity, Innate; D007234:Infant, Premature; D007235:Infant, Premature, Diseases / Q000097:blood* / Q000175:diagnosis / Q000276:immunology; D011247:Pregnancy; D011446:Prospective Studies; D018805:Sepsis / Q000097:blood* / Q000175:diagnosis; D013997:Time Factors
chemical_list: D015415:Biomarkers; D016207:Cytokines