title: Evaluation of histidine-rich glycoprotein tissue RNA and serum protein as novel markers for breast cancer.
abstract: Advances in the field of breast cancer (BC) biomarkers discovery facilitate diagnosis and treatment of BC in its pre-invasive state. While the genetic tissue markers are making significant advances in understanding the molecular basis of BC, serum has long been considered a rich source for biomarkers. So, integrated genomic and proteomic strategies play a huge role in the analytical validation of BC biomarkers and represent a true milestone in the areas of diagnostics and personalized medicine. This study included 60 cases (BC), 30 patients with fibroadenoma and 30 healthy women. Histidine-rich glycoprotein RNA (HRG) tissue expression was analyzed through gene expression-based outcome for breast cancer online algorithm (GOBO) bioinformatic analysis. To confirm our informatics analysis, HRG RNA was detected in breast tissue samples by RT-PCR, and HRG serum protein was estimated by ELISA. GOBO analysis revealed increased HRG RNA expression in all subtypes of BC with relative higher expression in basal subtype and grade 2. We confirmed these findings by HRG tissue RNA with 71.7% sensitivity and 93.3% specificity. HRG serum protein was 86.7% sensitivity and 80% specificity. HRG tissue RNA and serum protein could be considered as promising novel markers for prediction of BC prognosis.
authors.affiliation: Oncology Diagnostic Unit, Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, P.O. Box 11381, Abbassia, Cairo, Egypt.
authors.forename: Marwa
authors.initials: M
authors.lastname: Matboli
authors.forename: Sanaa
authors.initials: S
authors.lastname: Eissa
authors.forename: Hebatallah
authors.initials: H
authors.lastname: Said
mesh_terms: D000328:Adult; D000368:Aged; D000465:Algorithms; D014408:Biomarkers, Tumor / Q000097:blood; D001943:Breast Neoplasms / Q000378:metabolism*; D019295:Computational Biology; D005260:Female; D018226:Fibroadenoma / Q000097:blood; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008875:Middle Aged; D010865:Pilot Projects; D011237:Predictive Value of Tests; D011379:Prognosis; D011446:Prospective Studies; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics / Q000379:methods; D012680:Sensitivity and Specificity; D000099053:Histidine-Rich Glycoprotein
chemical_list: D014408:Biomarkers, Tumor; D011506:Proteins; D000099053:Histidine-Rich Glycoprotein