title: Kinetic characterization of Channa striatus muscle sarcoplasmic and myofibrillar protein hydrolysates.
abstract: This study was conducted to evaluate the kinetic characteristics of proteolytic activity of proteases on Channa striatus protein fractions. Degree of hydrolysis (DH), amino acid composition and kinetic parameters of sarcoplasmic and myofibrillar proteins were investigated when incubated with proteinase K and thermolysin, separately. After 30 min incubation with proteases, a decrease in DH of sarcoplasmic protein was observed whereas, hydrolysis of myofibrillar protein with proteases took 2 h with an increase in DH. The major amino acids were glutamic acid (16.6%) in thermolysin- myofibrillar hydrolysate followed by aspartic acid (11.1%) in sarcoplasmic protein fraction with no enzyme treatment and lysine (10%) in thermolysin-myofibrillar hydrolysate. The apparent Michaelis constant of proteinase K was lower than thermolysin for both sarcoplasmic and myofibrillar proteins. However, rate of turnover and enzyme efficiency suggested that sarcoplasmic and myofibrillar proteins are suitable substrates for proteinase K and thermolysin hydrolytic reaction, respectively.
authors.affiliation: School of Chemical Sciences and Food Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Malaysia.
authors.forename: Masomeh
authors.initials: M
authors.lastname: Ghassem
authors.affiliation: School of Chemical Sciences and Food Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Malaysia.
authors.forename: See Siau
authors.initials: SS
authors.lastname: Fern
authors.affiliation: School of Chemical Sciences and Food Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Malaysia.
authors.forename: Mamot
authors.initials: M
authors.lastname: Said
authors.affiliation: School of biosciences and biotechnology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Malaysia.
authors.forename: Zainon Mohd
authors.initials: ZM
authors.lastname: Ali
authors.affiliation: Fishery Research Institute, 11960 Batu Muang, Penang Malaysia.
authors.forename: Saadiah
authors.initials: S
authors.lastname: Ibrahim
authors.affiliation: School of Chemical Sciences and Food Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Malaysia.
authors.forename: Abdul Salam
authors.initials: AS
authors.lastname: Babji
keywords: Channa striatus; Degree of hydrolysis; Kinetic parameters; Michaelis constant; Proteolytic activity