title: Conserved residues in yeast initiator tRNA calibrate initiation accuracy by regulating preinitiation complex stability at the start codon.
abstract: Eukaryotic initiator tRNA (tRNAi) contains several highly conserved unique sequence features, but their importance in accurate start codon selection was unknown. Here we show that conserved bases throughout tRNAi, from the anticodon stem to acceptor stem, play key roles in ensuring the fidelity of start codon recognition in yeast cells. Substituting the conserved G31:C39 base pair in the anticodon stem with different pairs reduces accuracy (the Sui(-) [suppressor of initiation codon] phenotype), whereas eliminating base pairing increases accuracy (the Ssu(-) [suppressor of Sui(-)] phenotype). The latter defect is fully suppressed by a Sui(-) substitution of T-loop residue A54. These genetic data are paralleled by opposing effects of Sui(-) and Ssu(-) substitutions on the stability of methionylated tRNAi (Met-tRNA(i)) binding (in the ternary complex [TC] with eIF2-GTP) to reconstituted preinitiation complexes (PICs). Disrupting the C3:G70 base pair in the acceptor stem produces a Sui(-) phenotype and also reduces the rate of TC binding to 40S subunits in vitro and in vivo. Both defects are suppressed by an Ssu(-) substitution in eIF1A that stabilizes the open/P(OUT) conformation of the PIC that exists prior to start codon recognition. Our data indicate that these signature sequences of tRNA(i) regulate accuracy by distinct mechanisms, promoting the open/P(OUT) conformation of the PIC (for C3:G70) or destabilizing the closed/P(IN) state (for G31:C39 and A54) that is critical for start codon recognition.
authors.affiliation: Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA;
authors.forename: Jinsheng
authors.initials: J
authors.lastname: Dong
authors.forename: Antonio
authors.initials: A
authors.lastname: Munoz
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Kolitz
authors.forename: Adesh K
authors.initials: AK
authors.lastname: Saini
authors.forename: Wen-ling
authors.initials: WL
authors.lastname: Chiu
authors.forename: Hafsa
authors.initials: H
authors.lastname: Rahman
authors.forename: Jon R
authors.initials: JR
authors.lastname: Lorsch
authors.forename: Alan G
authors.initials: AG
authors.lastname: Hinnebusch
mesh_terms: D020029:Base Pairing; D018387:Codon, Initiator / Q000235:genetics*; D017124:Conserved Sequence; D009154:Mutation; D011487:Protein Conformation; D055550:Protein Stability; D012358:RNA, Transfer, Met / Q000235:genetics* / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D061785:Transcription Initiation, Genetic
chemical_list: D018387:Codon, Initiator; D012358:RNA, Transfer, Met
keywords: accuracy; initiation; initiator; scanning; tRNA; translation; yeast