title: Inhibition of 12/15 lipoxygenase by curcumin and an extract from Curcuma longa L.
abstract: Curcumin (diferuloylmethane) is an orange-yellow secondary metabolic compound from the rhizome of turmeric (Curcuma longa L.), a spice often found in curry powder. It is one of the major curcuminoids of turmeric. For centuries, curcumin has been used in some medicinal preparations or as a food colouring agent. A variety of enzymes that are closely associated with inflammation and cancer were found to be modulated by curcumin. This paper summarized the results of the inhibitory effect of curcumin and a Curcuma longa L. ethanolic extract on lipoxygenase from the rat lung cytosolic fraction. The positional specificity determination of arachidonic acid dioxygenation by RP- and SP-HPLC methods showed that in a purified enzyme preparation from the rat lung cytosol the specific form of lipoxygenase (LOX) is present exhibiting 12/15-LOX dual specificity (with predominant 15-LOX activity). The inhibitory activity of curcumin and Curcuma longa extract on LOX from cytosolic fraction of rat lung was expressed in the percentage of inhibition and as IC50. Lineweaver-Burk plot analysis has indicated that curcumin is the competitive inhibitor of 12/15 LOX from the rat lung cytosolic fraction.
authors.forename: Lýdia
authors.initials: L
authors.lastname: Bezáková
authors.forename: Daniela
authors.initials: D
authors.lastname: Košťálová
authors.forename: Marek
authors.initials: M
authors.lastname: Obložinský
authors.forename: Peter
authors.initials: P
authors.lastname: Hoffman
authors.forename: Mária
authors.initials: M
authors.lastname: Pekárová
authors.forename: Renáta
authors.initials: R
authors.lastname: Kollárová
authors.forename: Ivana
authors.initials: I
authors.lastname: Holková
authors.forename: Silvia
authors.initials: S
authors.lastname: Mošovská
authors.forename: Ernest
authors.initials: E
authors.lastname: Sturdík
mesh_terms: D000818:Animals; D001092:Arachidonate 12-Lipoxygenase / Q000187:drug effects*; D001093:Arachidonate 15-Lipoxygenase / Q000187:drug effects*; D016718:Arachidonic Acid / Q000737:chemistry; D030024:Curcuma / Q000737:chemistry*; D003474:Curcumin / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D027343:Rhizome
chemical_list: C475064:12-15-lipoxygenase; D016718:Arachidonic Acid; D001092:Arachidonate 12-Lipoxygenase; D001093:Arachidonate 15-Lipoxygenase; D003474:Curcumin