title: Mechanisms of hela cell apoptosis induced by abnormal Savda Munziq total phenolics combined with chemotherapeutic agents.
abstract: OBJECTIVE
  To investigate the effects of abnormal Savda Munziq (ASMq) total phenolics combined with cisplatin and docetaxel on the Hela cell growth.
  
  
  METHODS
  In vivo cultured Hela cells were treated with cisplatin, docetaxel, total phenolics, cisplatin+total phenolics or docetaxel+total phenolics. MTT was performed to assess inhibition of cell proliferation, flow cytometry to detect apoptosis, and semi-quantitative RT-PCR to test for survivin and Bcl-2 expression.
  
  
  RESULTS
  The total phenolics, cisplatin and docetaxel had significant inhibitory and apoptosis-promoting effects on Hela cells (P<0.05), with the early apoptotic rates of 12.8±0.70%, 18.9±3.79% and 15.8±3.8)%; the total phenolics, cisplatin and docetaxel significantly decreased the expression of Bcl-2 and survivin (all P<0.01), especially when used in combination.
  
  
  CONCLUSIONS
  ASMq total phenolics, combined with cisplatin and docetaxel, could promote the apoptosis of Hela cells possibly through reducing the expression of Bcl-2 and survivin.
authors.affiliation: The 6th Department of Gynecology, the Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, China E-mail : guzalnurabliz@163.com.
authors.forename: Yun-Xia
authors.initials: YX
authors.lastname: Zhang
authors.forename: Guzalnur
authors.initials: G
authors.lastname: Abliz
authors.forename: Wei-Jun
authors.initials: WJ
authors.lastname: Ye
authors.forename: Zuohelaguli
authors.initials: Z
authors.lastname: Mutalipu
authors.forename: Xiao-Wen
authors.initials: XW
authors.lastname: Li
authors.forename: Hai-Qin
authors.initials: HQ
authors.lastname: Wang
authors.forename: Gulimire
authors.initials: G
authors.lastname: Buranjiang
authors.forename: Halmurat
authors.initials: H
authors.lastname: Upur
mesh_terms: D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002945:Cisplatin / Q000008:administration & dosage; D000077143:Docetaxel; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077022:Survivin; D043823:Taxoids / Q000008:administration & dosage
chemical_list: C107489:BIRC5 protein, human; D004365:Drugs, Chinese Herbal; D051034:Inhibitor of Apoptosis Proteins; D010636:Phenols; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D000077022:Survivin; D043823:Taxoids; D000077143:Docetaxel; D002945:Cisplatin