title: Exploitation of starch industry liquid by-product to produce bioactive peptides from rice hydrolyzed proteins.
abstract: Small peptides show higher antioxidant capacity than native proteins and may be absorbed in the intestine without further digestion. In our study, a protein by-product from rice starch industry was hydrolyzed with commercial proteolytic enzymes (Alcalase, Neutrase, Flavourzyme) and microbial whole cells of Bacillus spp. and the released peptides were tested for antioxidant activity. Among enzymes, Alcalase was the most performing, while microbial proteolytic activity was less efficient. Conversely, the antioxidant activity was higher in the samples obtained by microbial hydrolysis and particularly with Bacillus pumilus AG1. The sequences of low molecular weight antioxidant peptides were determined and analyzed for aminoacidic composition. The results obtained so far suggest that the hydrolytic treatment of this industrial by-product, with selected enzymes and microbial systems, can allow its exploitation for the production of functional additives and supplements rich in antioxidant peptides, to be used in new food formulas for human consumption.
authors.affiliation: Department of Agri-Food Science and Technology (DISTAL), University of Bologna, Via Fanin, 50, 40127 Bologna, Italy.
authors.forename: Lucilla
authors.initials: L
authors.lastname: Dei Piu'
authors.affiliation: Department of Biological, Geological and Environmental Sciences, University of Bologna, Via Irnerio 42, 40126 Bologna, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Tassoni
authors.affiliation: Inter-departmental Center of Industrial Agri-Food Research (CIRI Agroalimentare), Piazza Goidanich 60, 47521 Cesena, Italy.
authors.forename: Diana Isabella
authors.initials: DI
authors.lastname: Serrazanetti
authors.affiliation: Department of Biological, Geological and Environmental Sciences, University of Bologna, Via Irnerio 42, 40126 Bologna, Italy.
authors.forename: Maura
authors.initials: M
authors.lastname: Ferri
authors.affiliation: Department of Agri-Food Science and Technology (DISTAL), University of Bologna, Via Fanin, 50, 40127 Bologna, Italy; Inter-departmental Center of Industrial Agri-Food Research (CIRI Agroalimentare), Piazza Goidanich 60, 47521 Cesena, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Babini
authors.affiliation: Department of Life Sciences, University of Modena and Reggio Emilia, via Amendola 2, 42122 Reggio Emilia, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Tagliazucchi
authors.affiliation: Department of Agri-Food Science and Technology (DISTAL), University of Bologna, Via Fanin, 50, 40127 Bologna, Italy; Inter-departmental Center of Industrial Agri-Food Research (CIRI Agroalimentare), Piazza Goidanich 60, 47521 Cesena, Italy. Electronic address: andrea.gianotti@unibo.it.
authors.forename: Andrea
authors.initials: A
authors.lastname: Gianotti
mesh_terms: D000595:Amino Acid Sequence; D000975:Antioxidants / Q000737:chemistry; D001407:Bacillus / Q000201:enzymology; D055162:Biocatalysis; D006801:Humans; D006868:Hydrolysis; D008969:Molecular Sequence Data; D012275:Oryza / Q000737:chemistry*; D010447:Peptide Hydrolases / Q000737:chemistry; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry*; D011492:Protein Hydrolysates / Q000737:chemistry*; D013213:Starch / Q000737:chemistry*; D014866:Waste Products / Q000032:analysis*
chemical_list: D000975:Antioxidants; D010455:Peptides; D011492:Protein Hydrolysates; D014866:Waste Products; D013213:Starch; D010447:Peptide Hydrolases
keywords: Antioxidant activity; Bacillus; Peptides; Proteases; Rice endosperm protein