title: Evaluation of different culture media for improvement in bioinsecticides production by indigenous Bacillus thuringiensis and their application against larvae of Aedes aegypti.
abstract: Production of indigenous isolate Bacillus thuringiensis sv2 (Bt sv2) was checked on conventional and nonconventional carbon and nitrogen sources in shake flasks. The effects on the production of biomass, toxin production, and spore formation capability of mosquito toxic strain were determined. Toxicity differs within the same strain depending on the growth medium. Bt sv2 produced with pigeon pea and soya bean flour were found highly effective with LC50 < 4 ppm against larvae of Aedes aegypti. These results were comparable with bacteria produced from Luria broth as a reference medium. Cost-effective analyses have revealed that production of biopesticide from test media is highly economical. The cost of production of Bt sv2 with soya bean flour was significantly reduced by 23-fold. The use of nonconventional sources has yielded a new knowledge in this area as the process development aspects of biomass production have been neglected as an area of research. These studies are very important from the point of media optimization for economic production of Bacillus thuringiensis based insecticides in mosquito control programmes.
authors.affiliation: School of Life Sciences, North Maharashtra University, P.O. Box 80, Jalgaon, Maharashtra 425001, India.
authors.forename: Patil Chandrashekhar
authors.initials: PC
authors.lastname: Devidas
authors.affiliation: School of Life Sciences, North Maharashtra University, P.O. Box 80, Jalgaon, Maharashtra 425001, India.
authors.forename: Borase Hemant
authors.initials: BH
authors.lastname: Pandit
authors.affiliation: School of Life Sciences, North Maharashtra University, P.O. Box 80, Jalgaon, Maharashtra 425001, India ; North Maharashtra Microbial Culture Collection Centre (NMCC), North Maharashtra University, P.O. Box 80, Jalgaon, Maharashtra 425001, India.
authors.forename: Patil Satish
authors.initials: PS
authors.lastname: Vitthalrao
mesh_terms: D000330:Aedes / Q000187:drug effects* / Q000254:growth & development; D000818:Animals; D001413:Bacillus thuringiensis / Q000254:growth & development / Q000378:metabolism*; D001427:Bacterial Toxins / Q000032:analysis* / Q000096:biosynthesis; D001709:Biotechnology / Q000379:methods; D003470:Culture Media / Q000737:chemistry* / Q000145:classification; D005285:Fermentation; D007306:Insecticides / Q000032:analysis*; D007814:Larva / Q000187:drug effects
chemical_list: D001427:Bacterial Toxins; D003470:Culture Media; D007306:Insecticides