title: Nitrogen removal via the nitrite pathway during wastewater co-treatment with ammonia-rich landfill leachates in a sequencing batch reactor.
abstract: The biological treatment of ammonia-rich landfill leachates due to an inadequate C to N ratio requires expensive supplementation of carbon from an external carbon source. In an effort to reduce treatment costs, the objective of the study was to determine the feasibility of nitrogen removal via the nitrite pathway during landfill leachate co-treatment with municipal wastewater. Initially, the laboratory-scale sequencing batch reactor (SBR) was inoculated with nitrifying activated sludge and fed only raw municipal wastewater (RWW) during a start-up period of 9 weeks. Then, in the co-treatment period, consisting of the next 17 weeks, the system was fed a mixture of RWW and an increasing quantity of landfill leachates (from 1 to 10% by volume). The results indicate that landfill leachate addition of up to 10% (by volume) influenced the effluent quality, except for BOD5. During the experiment, a positive correlation (r(2) = 0.908) between ammonia load in the influent and nitrite in the effluent was observed, suggesting that the second step of nitrification was partially inhibited. The partial nitrification (PN) was also confirmed by fluorescence in situ hybridisation (FISH) analysis of nitrifying bacteria. Nitrogen removal via the nitrite pathway was observed when the oxygen concentration ranged from 0.5 to 1.5 mg O2/dm(3) and free ammonia (FA) ranged from 2.01 to 35.86 mg N-NH3/dm(3) in the aerobic phase. Increasing ammonia load in wastewater influent was also correlated with an increasing amount of total nitrogen (TN) in the effluent, which suggested insufficient amounts of assimilable organic carbon to complete denitrification. Because nitrogen removal via the nitrite pathway is beneficial for carbon-limited and highly ammonia-loaded mixtures, obtaining PN can lead to a reduction in the external carbon source needed to support denitrification.
authors.affiliation: Sanitary Engineering, Faculty of Civil and Environmental Engineering, Gdansk University of Technology, Gabriela Narutowicza Str. 11/12, 80-233, Gdansk, Poland, sksiazek@pg.gda.pl.
authors.forename: S
authors.initials: S
authors.lastname: Fudala-Ksiazek
authors.forename: A
authors.initials: A
authors.lastname: Luczkiewicz
authors.forename: K
authors.initials: K
authors.lastname: Fitobor
authors.forename: K
authors.initials: K
authors.lastname: Olanczuk-Neyman
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism*; D020562:Betaproteobacteria / Q000378:metabolism; D019149:Bioreactors / Q000382:microbiology*; D002244:Carbon / Q000737:chemistry; D058440:Denitrification; D005240:Feasibility Studies; D017404:In Situ Hybridization, Fluorescence; D058465:Nitrification; D009573:Nitrites / Q000378:metabolism*; D009576:Nitrobacter / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D012722:Sewage / Q000737:chemistry* / Q000382:microbiology; D062065:Wastewater / Q000737:chemistry* / Q000382:microbiology; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D000641:Ammonia; D002244:Carbon; D009573:Nitrites; D009584:Nitrogen; D012722:Sewage; D062065:Wastewater; D014874:Water Pollutants, Chemical