title: Nuclear receptor 4a3 (nr4a3) regulates murine mast cell responses and granule content.
abstract: Nuclear receptor 4a3 (Nr4a3) is a transcription factor implicated in various settings such as vascular biology and inflammation. We have recently shown that mast cells dramatically upregulate Nuclear receptor 4a3 upon activation, and here we investigated the functional impact of Nuclear receptor 4a3 on mast cell responses. We show that Nuclear receptor 4a3 is involved in the regulation of cytokine/chemokine secretion in mast cells following activation via the high affinity IgE receptor. Moreover, Nuclear receptor 4a3 negatively affects the transcript and protein levels of mast cell tryptase as well as the mast cell's responsiveness to allergen. Together, these findings identify Nuclear receptor 4a3 as a novel regulator of mast cell function.
authors.affiliation: Swedish University of Agricultural Sciences, Department of Anatomy, Physiology and Biochemistry, BMC, Uppsala, Sweden.
authors.forename: Gianni
authors.initials: G
authors.lastname: Garcia-Faroldi
authors.affiliation: Swedish University of Agricultural Sciences, Department of Anatomy, Physiology and Biochemistry, BMC, Uppsala, Sweden.
authors.forename: Fabio R
authors.initials: FR
authors.lastname: Melo
authors.affiliation: Saha Cardiovascular Research Center, University of Kentucky College of Medicine, Wethington, Kentucky, United States of America.
authors.forename: Dennis
authors.initials: D
authors.lastname: Bruemmer
authors.affiliation: Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, United States of America.
authors.forename: Orla M
authors.initials: OM
authors.lastname: Conneely
authors.affiliation: Swedish University of Agricultural Sciences, Department of Anatomy, Physiology and Biochemistry, BMC, Uppsala, Sweden.
authors.forename: Gunnar
authors.initials: G
authors.lastname: Pejler
authors.affiliation: Swedish University of Agricultural Sciences, Department of Anatomy, Physiology and Biochemistry, BMC, Uppsala, Sweden.
authors.forename: Anders
authors.initials: A
authors.lastname: Lundequist
mesh_terms: D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000276:immunology / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D018925:Chemokines / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D003594:Cytoplasmic Granules / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000502:physiology*; D007073:Immunoglobulin E / Q000494:pharmacology; D008407:Mast Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D017455:Receptors, IgE / Q000235:genetics / Q000378:metabolism; D011987:Receptors, Steroid / Q000502:physiology*; D011988:Receptors, Thyroid Hormone / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects; D053802:Tryptases / Q000235:genetics / Q000378:metabolism
chemical_list: D018925:Chemokines; D016207:Cytokines; D004268:DNA-Binding Proteins; D016328:NF-kappa B; D009419:Nerve Tissue Proteins; C490411:Nr4a3 protein, mouse; D012333:RNA, Messenger; D017455:Receptors, IgE; D011987:Receptors, Steroid; D011988:Receptors, Thyroid Hormone; D007073:Immunoglobulin E; D053802:Tryptases