title: Preparation and characterization of antimicrobial nano-hydroxyapatite composites.
abstract: Deep infection of prosthesis is one of the most frequent complications after joint replacement. One of the most effective ways is to introduce directly some antibiotics in the local site of the surgery. In the present study, an antimicrobial composite has been fabricated using nano-hydroxyapatite particles as carriers for the antimicrobial drug of vancomycin hydrochloride (VAN) and the mixture of oxidation sodium alginate (OSA) and gelatin (GT) as a sticky matrix. Samples have been characterized using X-ray diffraction instrument (XRD), field emission scanning electron microscope (FE-SEM), transmission electron microscope (TEM) and Fourier transform infrared (FTIR) spectra, Brunauer-Emmett-Teller (BET) methods, the rotational rheometer and the texture analyzer. The release of VAN from nano-hydroxyapatite (nHAP) particles was detected by the ultraviolet-visible (UV-vis) spectrophotometer and then bactericidal property of the composite was evaluated using the Staphylococcus aureus (S. aureus) as a bacterial model. Experimental results showed that the composite possessed an adhesive property derived from the gel of OSA and GT, which implied that the composite could bond directly to the fracture surface of bones in surgery. Furthermore, VAN was loaded efficiently on the surface of nHAP particles and could be released slowly from these particles, which endowed the composite with an obvious and continuous antimicrobial performance. The sticky and antimicrobial composite may has a potential application in arthroplasty to overcome deep infection in a simple and direct manner.
authors.affiliation: The Key Laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education, College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou 310018, China.
authors.forename: Juhong
authors.initials: J
authors.lastname: Yu
authors.affiliation: The First Affiliated Hospital, Zhejiang Chinese Medicine University, Hangzhou 310006, China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Chu
authors.affiliation: The Key Laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education, College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou 310018, China.
authors.forename: Yurong
authors.initials: Y
authors.lastname: Cai
authors.affiliation: The First Affiliated Hospital, Zhejiang Chinese Medicine University, Hangzhou 310006, China.
authors.forename: Peijian
authors.initials: P
authors.lastname: Tong
authors.affiliation: The Key Laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education, College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou 310018, China. Electronic address: yaoj@zstu.edu.cn.
authors.forename: Juming
authors.initials: J
authors.lastname: Yao
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects; D014640:Vancomycin / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D004337:Drug Carriers; D014640:Vancomycin; D017886:Durapatite
keywords: Antibacterial; Drug release; Nano-hydroxyapatite; Vancomycin hydrochloride