title: Bloodstream infections by Malassezia and Candida species in critical care patients.
abstract: Despite being considered an emerging yeast related to immunocompromised individuals, severe infections by Malassezia furfur have not been evaluated. During a one-year survey on yeasts fungemia, 290 neonatal and 17 pediatric patients with intravascular catheters, lipid parenteral nutrition, prolonged ward stay, and surgery were enrolled. In addition, the origin of the infection was investigated by swabbing hand skin of patients, parents, and healthcare workers and medical devices. All biological specimens and swabs were cultured on Sabouraud dextrose agar and Dixon agar. The yeasts identification was based on morphological and biochemical features and by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and confirmed by sequencing the internal transcribed spacer of nuclear ribosomal DNA. A higher prevalence of M. furfur (2.1%) over Candida spp. (1.4%) caused bloodstream infections (BSIs). Twelve fungemia episodes were recorded: 2 by M. furfur in a pediatric ward and 10 in a neonatal intensive care unit (6 caused by M. furfur and 4 by Candida spp.). M. furfur was also isolated from the skin of all patients with BSIs, from the hand skin of a parent, and from an incubator surface and sheet. Patients with Candida spp. and M. furfur BSIs were successfully treated with intravenous liposomal Amphotericin B. These findings highlight the need for a more accurate etiological diagnosis in high-risk patients by adding lipid-supplemented culture media for Malassezia in the current mycological routine as the clinical features, patient management, and outcomes in both Candida and Malassezia fungemia do not differ.
authors.affiliation: Dipartimento di Medicina Veterinaria, Università degli Studi "Aldo Moro," Valenzano.
authors.forename: Roberta
authors.initials: R
authors.lastname: Iatta
authors.forename: Claudia
authors.initials: C
authors.lastname: Cafarchia
authors.forename: Teresa
authors.initials: T
authors.lastname: Cuna
authors.forename: Osvaldo
authors.initials: O
authors.lastname: Montagna
authors.forename: Nicola
authors.initials: N
authors.lastname: Laforgia
authors.forename: Ottavio
authors.initials: O
authors.lastname: Gentile
authors.forename: Antonino
authors.initials: A
authors.lastname: Rizzo
authors.forename: Teun
authors.initials: T
authors.lastname: Boekhout
authors.forename: Domenico
authors.initials: D
authors.lastname: Otranto
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Montagna
mesh_terms: D000293:Adolescent; D000666:Amphotericin B / Q000627:therapeutic use; D002175:Candida / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D003422:Critical Care; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D005260:Female; D016469:Fungemia / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008290:Malassezia / Q000302:isolation & purification*; D008297:Male; D008828:Microbiological Techniques; D008969:Molecular Sequence Data; D015995:Prevalence; D017422:Sequence Analysis, DNA; D016896:Treatment Outcome
chemical_list: D004271:DNA, Fungal; D000666:Amphotericin B
keywords: Malassezia furfur; fungal bloodstream infection; neonates