title: Characteristics of collagens from the swim bladders of yellowfin tuna (Thunnus albacares).
abstract: Acid soluble collagen (ASC) and pepsin soluble collagen (PSC) were extracted from the swim bladders of yellowfin tuna (Thunnus albacares) with yields of 1.07% and 12.10%, respectively. Based on electrophoretic patterns, both ASC and PSC consisted of two α-chains (α1 and α2) and were characterised to be type I collagen. ASC had higher β-chains than PSC. Imino acid contents of ASC and PSC were 128 and 169 residues/1,000 residues, respectively. Fourier transform infrared (FTIR) spectra of both ASC and PSC were similar and revealed the presence of a triple helix. Both ASC and PSC had the highest solubility at acidic pHs. From zeta potential analysis, a net charge of zero was found at pH 6.05 and 5.93 for ASC and PSC, respectively. Tmax of ASC and PSC were 32.97 and 33.92°C, respectively. The swim bladders of yellowfin tuna could therefore serve as an alternative source of collagen for future applications.
authors.affiliation: Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
authors.forename: Onouma
authors.initials: O
authors.lastname: Kaewdang
authors.affiliation: Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand. Electronic address: soottawat.b@psu.ac.th.
authors.forename: Soottawat
authors.initials: S
authors.lastname: Benjakul
authors.affiliation: Department of Food Science and Nutrition, Faculty of Science and Technology, Prince of Songkla University, Pattani 94000, Thailand.
authors.forename: Thammarat
authors.initials: T
authors.lastname: Kaewmanee
authors.affiliation: Laboratory of Marine Products and Food Science, Research Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Kishimura
mesh_terms: D000825:Animal Structures / Q000737:chemistry*; D000818:Animals; D003094:Collagen / Q000737:chemistry*; D029941:Fish Proteins / Q000737:chemistry*; D006868:Hydrolysis; D055550:Protein Stability; D012995:Solubility; D014413:Tuna; D014866:Waste Products / Q000032:analysis*
chemical_list: D029941:Fish Proteins; D014866:Waste Products; D003094:Collagen
keywords: Acid soluble collagen; Pepsin soluble collagen; Swim bladders; Yellowfin tuna