title: Lesion profiling and subcellular prion localization of cervid chronic wasting disease in domestic cats.
abstract: Chronic wasting disease (CWD) is an efficiently transmitted, fatal, and progressive prion disease of cervids with an as yet to be fully clarified host range. While outbred domestic cats (Felis catus) have recently been shown to be susceptible to experimental CWD infection, the neuropathologic features of the infection are lacking. Such information is vital to provide diagnostic power in the event of natural interspecies transmission and insights into host and strain interactions in interspecies prion infection. Using light microscopy and immunohistochemistry, we detail the topographic pattern of neural spongiosis (the "lesion profile") and the distribution of misfolded prion protein in the primary and secondary passage of feline CWD (Fel(CWD)). We also evaluated cellular and subcellular associations between misfolded prion protein (PrP(D)) and central nervous system neurons and glial cell populations. From these studies, we (1) describe the novel neuropathologic profile of Fel(CWD), which is distinct from either cervid CWD or feline spongiform encephalopathy (FSE), and (2) provide evidence of serial passage-associated interspecies prion adaptation. In addition, we demonstrate through confocal analysis the successful co-localization of PrP(D) with neurons, astrocytes, microglia, lysosomes, and synaptophysin, which, in part, implicates each of these in the neuropathology of Fel(CWD). In conclusion, this work illustrates the simultaneous role of both host and strain in the development of a unique Fel(CWD) neuropathologic profile and that such a profile can be used to discriminate between Fel(CWD) and FSE.
authors.affiliation: Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO, USA dseelig@umn.edu.
authors.forename: D M
authors.initials: DM
authors.lastname: Seelig
authors.affiliation: Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO, USA.
authors.forename: A V
authors.initials: AV
authors.lastname: Nalls
authors.affiliation: Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, USA.
authors.forename: M
authors.initials: M
authors.lastname: Flasik
authors.affiliation: Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO, USA.
authors.forename: V
authors.initials: V
authors.lastname: Frank
authors.affiliation: Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, USA.
authors.forename: S
authors.initials: S
authors.lastname: Eaton
authors.affiliation: Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO, USA.
authors.forename: C K
authors.initials: CK
authors.lastname: Mathiason
authors.affiliation: Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO, USA.
authors.forename: E A
authors.initials: EA
authors.lastname: Hoover
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism / Q000473:pathology; D002371:Cat Diseases / Q000378:metabolism / Q000473:pathology*; D002415:Cats; D002490:Central Nervous System / Q000378:metabolism / Q000473:pathology; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D011328:Prions / Q000502:physiology*; D012692:Serial Passage / Q000662:veterinary; D016708:Synaptophysin / Q000378:metabolism; D034081:Wasting Disease, Chronic / Q000473:pathology*
chemical_list: D011328:Prions; D016708:Synaptophysin
keywords: adaptation; cat; chronic wasting disease; feline spongiform encephalopathy; immunohistochemistry; interspecies; prion; species barrier; transmissible spongiform encephalopathy