title: Solid State Fermentation of a Raw Starch Digesting Alkaline Alpha-Amylase from Bacillus licheniformis RT7PE1 and Its Characteristics.
abstract: The thermodynamic and kinetic properties of solids state raw starch digesting alpha amylase from newly isolated Bacillus licheniformis RT7PE1 strain were studied. The kinetic values Q p , Y p/s , Y p/X , and q p were proved to be best with 15% wheat bran. The molecular weight of purified enzyme was 112 kDa. The apparent K m and V max values for starch were 3.4 mg mL(-1) and 19.5 IU mg(-1) protein, respectively. The optimum temperature and pH for α -amylase were 55°C, 9.8. The half-life of enzyme at 95°C was 17h. The activation and denaturation activation energies were 45.2 and 41.2 kJ mol(-1), respectively. Both enthalpies (ΔH (∗)) and entropies of activation (ΔS (∗)) for denaturation of α -amylase were lower than those reported for other thermostable α -amylases.
authors.affiliation: National Institute for Biotechnology and Genetic Engineering (NIBGE), P.O. Box 577, Faisalabad, Pakistan.
authors.forename: Romana
authors.initials: R
authors.lastname: Tabassum
authors.affiliation: National Institute for Biotechnology and Genetic Engineering (NIBGE), P.O. Box 577, Faisalabad, Pakistan.
authors.forename: Shazia
authors.initials: S
authors.lastname: Khaliq
authors.affiliation: Department of Bioinformatics, G C University, Faisalabad, Pakistan.
authors.forename: Muhammad Ibrahim
authors.initials: MI
authors.lastname: Rajoka
authors.affiliation: Biological Engineering Department, Utah State University, 4105 Old Main Hill, Logan, UT 84322-4105, USA.
authors.forename: Foster
authors.initials: F
authors.lastname: Agblevor