title: Dexamethasone effects on vascular flow and organ injury in septic mice.
abstract: BACKGROUND
  To demonstrate the effects of low-dose dexamethasone treatment on mesenteric artery blood flow, oxidative injury, vascular reactivity, and survival in Swiss albino mice with intra-abdominal polymicrobial sepsis accomplished by cecal ligation and puncture (CLP).
  
  
  METHODS
  Mice were allocated to CLP + saline, CLP + dexamethasone, sham + saline, and sham + dexamethasone subgroups to evaluate blood flow, organ injury, and vascular response to consecutive phenylephrine administrations at 24, 48, and 72 h. Survival rates were also evaluated in a different group of mice. Dexamethasone (1 mg/kg/d) and saline (4 mL/kg/d) were administered intraperitoneally to mice 2 h after CLP or sham procedure, whichever appropriate, and repeated once a day until evaluation time at 48 and 72 h. Relaparotomy was performed at the concerned time and mesenteric blood flow was measured, and liver, lung, and peritoneum samples were obtained. Alteration in mesenteric blood flow response to intravenous phenylephrine injections was recorded at the related time intervals in different mice groups. Survival group was followed up by 7-d administration of dexamethasone or saline for 18 d.
  
  
  RESULTS
  The significant fall in mesenteric blood flow after CLP ameliorated with dexamethasone treatment at 48 and 72 h. Dexamethasone also diminished the malonyl dialdehyde level, which is an indicator of organ injury raised after CLP, at 24 h in liver, lung, and peritoneum samples. Dexamethasone therapy has significantly enhanced the vascular response to phenylephrine injections at all doses; however, no change was observed in survival rates.
  
  
  CONCLUSIONS
  Low-dose dexamethasone has beneficial effects on mesenteric blood flow and organ injury in experimental sepsis models.
authors.affiliation: Department of General Surgery, Baskent University Faculty of Medicine, Adana Teaching and Research Hospital, Adana, Turkey. Electronic address: ozguraytc@hotmail.com.
authors.forename: Huseyin Ozgur
authors.initials: HO
authors.lastname: Aytac
authors.affiliation: Department of Pharmacology, Hacettepe University Faculty of Medicine, Ankara, Turkey.
authors.forename: Alper B
authors.initials: AB
authors.lastname: Iskit
authors.affiliation: Department of General Surgery, Hacettepe University Faculty of Medicine, Ankara, Turkey.
authors.forename: Iskender
authors.initials: I
authors.lastname: Sayek
mesh_terms: D000818:Animals; D003907:Dexamethasone / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D005260:Female; D005938:Glucocorticoids / Q000494:pharmacology / Q000627:therapeutic use*; D008315:Malondialdehyde / Q000378:metabolism; D008638:Mesenteric Arteries / Q000187:drug effects*; D051379:Mice; D010656:Phenylephrine; D012039:Regional Blood Flow / Q000187:drug effects*; D018805:Sepsis / Q000188:drug therapy* / Q000378:metabolism; D014662:Vasoconstrictor Agents
chemical_list: D005938:Glucocorticoids; D014662:Vasoconstrictor Agents; D010656:Phenylephrine; D008315:Malondialdehyde; D003907:Dexamethasone
keywords: Mesenteric blood flow; Organ injury; Sepsis; Steroid; Vascular hyporeactivity