title: General assessment of copy number variation in normal and tumor tissues of the domestic dog (Canis lupus familiaris).
abstract: In recent years, characterization of a copy number variation (CNV) of the genomic DNA has provided evidence for the relationship of this type of genetic variation with the occurrence of a broad spectrum of diseases, including cancer lesions. Copy number variants (CNVs) also occur in the genomes of healthy individuals as a result of abnormal recombination processes in germ cells and have a hereditary character contributing to the natural genetic diversity. Recent image analysis methods and advanced computational techniques allow for identification of CNVs using SNPs genotyping microarrays based on the analysis of signal intensity observed for markers located in the specific genomic regions. In this study we used CanineHD BeadChip assay (Illumina) to identify both natural and cancer-induced CNVs in the genomes of different dog breeds and in different cancer types occurring in this species. The obtained results showed that structural aberrations are a common phenomenon arising during a tumor progression and are more complex and widespread in tumors of mesenchymal tissue origin than in epithelial tissue originating tumors. The tumor derived CNVs, in comparison to healthy samples, were characterized by larger sizes of regions, higher number of amplifications, and in some cases encompassed genes with potential effect on tumor progression.
authors.affiliation: Laboratory of Genomics, National Research Institute of Animal Production, Krakowska 1, 32-083, Balice n. Krakow, Poland, artur.gurgul@izoo.krakow.pl.
authors.forename: Artur
authors.initials: A
authors.lastname: Gurgul
authors.forename: Kacper
authors.initials: K
authors.lastname: Żukowski
authors.forename: Brygida
authors.initials: B
authors.lastname: Ślaska
authors.forename: Ewelina
authors.initials: E
authors.lastname: Semik
authors.forename: Klaudia
authors.initials: K
authors.lastname: Pawlina
authors.forename: Tomasz
authors.initials: T
authors.lastname: Ząbek
authors.forename: Igor
authors.initials: I
authors.lastname: Jasielczuk
authors.forename: Monika
authors.initials: M
authors.lastname: Bugno-Poniewierska
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics*; D002874:Chromosome Mapping; D056915:DNA Copy Number Variations / Q000235:genetics*; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D005260:Female; D016678:Genome; D023281:Genomics; D008297:Male; D009369:Neoplasms / Q000235:genetics / Q000662:veterinary*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor