title: [Decalcification in formic acid after fixation in zinc-ethanol-formaldehyde does not preclude identification of neuronal and glial markers].
abstract: The aim of this study was to determine the possibility of immunohistochemical demonstration of various tissue antigens in paraffin sections of zinc-ethanol-formaldehyde-fixed spinal cord, surrounded by the vertebral tissues and adjacent muscles, after decalcification in formic acid, in adult (n = 5) and newborn (n = 2) Wistar rats. In the present work, the antibodies used were against: glial fibrillary acidic protein, neurofilaments, neuronal specific nuclear protein, enzyme tyrosine hydroxylase, protein of synaptic vesicles--synaptophysin and the intermediate filament protein--vimentin. Combination of fixation in zinc-ethanol-formaldehyde and decalcification in 21% formic acid solution was shown to provide an optimal preservation of the antigens studied.
authors.forename: E A
authors.initials: EA
authors.lastname: Kolos
authors.forename: D E
authors.initials: DE
authors.lastname: Korzhevskiĭ
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D003648:Decalcification Technique / Q000379:methods*; D000431:Ethanol / Q000737:chemistry; D005404:Fixatives / Q000737:chemistry; D005557:Formaldehyde / Q000737:chemistry; D005561:Formates / Q000737:chemistry; D005904:Glial Fibrillary Acidic Protein / Q000737:chemistry / Q000276:immunology; D006652:Histological Techniques; D007150:Immunohistochemistry / Q000379:methods*; D009457:Neuroglia / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015032:Zinc / Q000737:chemistry
chemical_list: D000906:Antibodies; D005404:Fixatives; D005561:Formates; D005904:Glial Fibrillary Acidic Protein; C030544:formic acid; D005557:Formaldehyde; D000431:Ethanol; D015032:Zinc