title: Genetic algorithm-artificial neural network and adaptive neuro-fuzzy inference system modeling of antibacterial activity of annatto dye on Salmonella enteritidis.
abstract: Annatto is commonly used as a coloring agent in the food industry and has antimicrobial and antioxidant properties. In this study, genetic algorithm-artificial neural network (GA-ANN) and adaptive neuro-fuzzy inference system (ANFIS) models were used to predict the effect of annatto dye on Salmonella enteritidis in mayonnaise. The GA-ANN and ANFIS were fed with 3 inputs of annatto dye concentration (0, 0.1, 0.2 and 0.4%), storage temperature (4 and 25°C) and storage time (1-20 days) for prediction of S. enteritidis population. Both models were trained with experimental data. The results showed that the annatto dye was able to reduce of S. enteritidis and its effect was stronger at 25°C than 4°C. The developed GA-ANN, which included 8 hidden neurons, could predict S. enteritidis population with correlation coefficient of 0.999. The overall agreement between ANFIS predictions and experimental data was also very good (r=0.998). Sensitivity analysis results showed that storage temperature was the most sensitive factor for prediction of S. enteritidis population.
authors.affiliation: Faculty of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. Electronic address: mahmud.yolmeh@yahoo.com.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Yolmeh
authors.affiliation: Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran. Electronic address: habibi@ac.um.ir.
authors.forename: Mohammad B
authors.initials: MB
authors.lastname: Habibi Najafi
authors.affiliation: Faculty of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. Electronic address: Fs1446@yahoo.com.
authors.forename: Fakhreddin
authors.initials: F
authors.lastname: Salehi
mesh_terms: D000465:Algorithms; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D031212:Bixaceae; D002338:Carotenoids / Q000494:pharmacology*; D005516:Food Microbiology; D061353:Food Storage; D008954:Models, Biological; D016571:Neural Networks, Computer; D010936:Plant Extracts / Q000494:pharmacology*; D012477:Salmonella enteritidis / Q000187:drug effects* / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D002338:Carotenoids; C054041:annatto
keywords: Annatto dye; Genetic algorithm; Mayonnaise; Salmonella enteritidis; Sensitivity analysis