title: Impact of PCOS on early embryo cleavage kinetics.
abstract: This study investigated whether polycystic ovary syndrome (PCOS) affected early embryo development assessed by time-lapse analysis of embryo kinetics from fertilization to the blastocyst stage. This was a prospective cohort study of two pronuclei (2PN) embryos from 25 hyperandrogenic PCOS patients (110 2PN embryos), 26 normoandrogenic PCOS patients (140 2PN embryos) and 20 healthy, regularly cycling women (controls, 97 2PN embryos). Patients underwent the same baseline evaluation and the same ovarian stimulation from April 2010 to February 2013. Oocytes were fertilized by intracytoplasmic sperm injection and incubated in an EmbryoScope with pictures taken every 20 min in seven focal planes. Time to 2PN breakdown, first cleavage and cleavage to 3, 4, 5, 6, 7 and 8 cells, morula and blastocyst (t₂, t₃, t₄, t₅, t₆, t₇, t₈, t(M), t(B)) were annotated. Differences in embryo kinetics between groups were assessed by mixed modelling. Compared with controls, embryos from hyperandrogenic PCOS patients were significantly delayed at 2PN breakdown, t₂, t₃, t₄ and t₇ but not at t₅, t₆, t₈, t(M) or t(B). Embryos from hyperandrogenic PCOS women had developed slower from fertilization to the 8-cell stage compared with embryos from controls.
authors.affiliation: Holbaek Fertility Clinic, Smedelundsgade 60, 4300 Holbaek, Denmark. Electronic address: mlwi@regionsjaelland.dk.
authors.forename: M L
authors.initials: ML
authors.lastname: Wissing
authors.affiliation: Holbaek Fertility Clinic, Smedelundsgade 60, 4300 Holbaek, Denmark.
authors.forename: M R
authors.initials: MR
authors.lastname: Bjerge
authors.affiliation: Holbaek Fertility Clinic, Smedelundsgade 60, 4300 Holbaek, Denmark.
authors.forename: A I G
authors.initials: AI
authors.lastname: Olesen
authors.affiliation: Holbaek Fertility Clinic, Smedelundsgade 60, 4300 Holbaek, Denmark.
authors.forename: T
authors.initials: T
authors.lastname: Hoest
authors.affiliation: Holbaek Fertility Clinic, Smedelundsgade 60, 4300 Holbaek, Denmark.
authors.forename: A L
authors.initials: AL
authors.lastname: Mikkelsen
mesh_terms: D000328:Adult; D047108:Embryonic Development; D005260:Female; D006801:Humans; D017588:Hyperandrogenism / Q000503:physiopathology; D011085:Polycystic Ovary Syndrome / Q000503:physiopathology*; D011247:Pregnancy; D011446:Prospective Studies; D020554:Sperm Injections, Intracytoplasmic; D059008:Time-Lapse Imaging; D017388:Zygote Intrafallopian Transfer
keywords: PCOS; cleavage kinetics; embryo kinetics; hyperandrogenaemia; testosterone; time lapse