title: MicroRNA-mRNA functional pairs for cisplatin resistance in ovarian cancer cells.
abstract: Ovarian cancer is the leading cause of death in women worldwide. Cisplatin is the core of first-line chemotherapy for patients with advanced ovarian cancer. Many patients eventually become resistant to cisplatin, diminishing its therapeutic effect. MicroRNAs (miRNAs) have critical functions in diverse biological processes. Using miRNA profiling and polymerase chain reaction validation, we identified a panel of differentially expressed miRNAs and their potential targets in cisplatin-resistant SKOV3/DDP ovarian cancer cells relative to cisplatin-sensitive SKOV3 parental cells. More specifically, our results revealed significant changes in the expression of 13 of 663 miRNAs analyzed, including 11 that were up-regulated and 2 that were down-regulated in SKOV3/DDP cells with or without cisplatin treatment compared with SKOV3 cells with or without cisplatin treatment. miRNA array and mRNA array data were further analyzed using Ingenuity Pathway Analysis software. Bioinformatics analysis suggests that the genes ANKRD17, SMC1A, SUMO1, GTF2H1, and TP73, which are involved in DNA damage signaling pathways, are potential targets of miRNAs in promoting cisplatin resistance. This study highlights candidate miRNA-mRNA interactions that may contribute to cisplatin resistance in ovarian cancer.
authors.affiliation: Laboratory of Cell and Molecular Biology & State Key Laboratory of Molecular Oncology, Cancer Institute & Cancer Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, P. R. China. xningzhi@public.bta.net.cn.
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Chen-Fei
authors.initials: CF
authors.lastname: Hu
authors.forename: Qing
authors.initials: Q
authors.lastname: Xu
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Zhu
authors.forename: Ning-Zhi
authors.initials: NZ
authors.lastname: Xu
mesh_terms: D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D002868:Chromosomal Proteins, Non-Histone; D002945:Cisplatin; D003546:Cysteine Endopeptidases; D004268:DNA-Binding Proteins; D015536:Down-Regulation; D019008:Drug Resistance, Neoplasm; D010450:Endopeptidases; D005260:Female; D006801:Humans; D035683:MicroRNAs; D009687:Nuclear Proteins; D010051:Ovarian Neoplasms; D010750:Phosphoproteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D015398:Signal Transduction; D051758:Transcription Factor TFIIH; D019473:Transcription Factors, TFII; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins; D015854:Up-Regulation; D000098550:Structural Maintenance of Chromosome Protein 1
chemical_list: C481273:ANKRD17 protein, human; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; C072546:GTF2H1 protein, human; D035683:MicroRNAs; D009687:Nuclear Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C000606460:TP73 protein, human; D019473:Transcription Factors, TFII; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins; D000098550:Structural Maintenance of Chromosome Protein 1; D051758:Transcription Factor TFIIH; D010450:Endopeptidases; C404512:SENP1 protein, human; D003546:Cysteine Endopeptidases; D002945:Cisplatin