title: HIV-associated disruption of tight and adherens junctions of oral epithelial cells facilitates HSV-1 infection and spread.
abstract: Herpes simplex virus (HSV) types 1 and 2 are the most common opportunistic infections in HIV/AIDS. In these immunocompromised individuals, HSV-1 reactivates and replicates in oral epithelium, leading to oral disorders such as ulcers, gingivitis, and necrotic lesions. Although the increased risk of HSV infection may be mediated in part by HIV-induced immune dysfunction, direct or indirect interactions of HIV and HSV at the molecular level may also play a role. In this report we show that prolonged interaction of the HIV proteins tat and gp120 and cell-free HIV virions with polarized oral epithelial cells leads to disruption of tight and adherens junctions of epithelial cells through the mitogen-activated protein kinase signaling pathway. HIV-induced disruption of oral epithelial junctions facilitates HSV-1 paracellular spread between the epithelial cells. Furthermore, HIV-associated disruption of adherens junctions exposes sequestered nectin-1, an adhesion protein and critical receptor for HSV envelope glycoprotein D (gD). Exposure of nectin-1 facilitates binding of HSV-1 gD, which substantially increases HSV-1 infection of epithelial cells with disrupted junctions over that of cells with intact junctions. Exposed nectin-1 from disrupted adherens junctions also increases the cell-to-cell spread of HSV-1 from infected to uninfected oral epithelial cells. Antibodies to nectin-1 and HSV-1 gD substantially reduce HSV-1 infection and cell-to-cell spread, indicating that HIV-promoted HSV infection and spread are mediated by the interaction of HSV gD with HIV-exposed nectin-1. Our data suggest that HIV-associated disruption of oral epithelial junctions may potentiate HSV-1 infection and its paracellular and cell-to-cell spread within the oral mucosal epithelium. This could be one of the possible mechanisms of rapid development of HSV-associated oral lesions in HIV-infected individuals.
authors.affiliation: Department of Medicine, University of California San Francisco, San Francisco, California, United States of America.
authors.forename: Irna
authors.initials: I
authors.lastname: Sufiawati
authors.affiliation: Department of Medicine, University of California San Francisco, San Francisco, California, United States of America ; Department of Orofacial Sciences, University of California San Francisco, San Francisco, California, United States of America.
authors.forename: Sharof M
authors.initials: SM
authors.lastname: Tugizov
mesh_terms: D015153:Blotting, Western; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000473:pathology* / Q000821:virology; D005455:Fluorescent Antibody Technique; D015696:Gene Products, tat / Q000378:metabolism; D015699:HIV Envelope Protein gp120 / Q000378:metabolism; D015658:HIV Infections / Q000150:complications*; D006561:Herpes Simplex / Q000209:etiology / Q000635:transmission*; D006801:Humans; D020935:MAP Kinase Signaling System / Q000502:physiology*; D009055:Mouth / Q000166:cytology*; D000075983:Nectins; D019108:Tight Junctions / Q000378:metabolism / Q000473:pathology* / Q000821:virology; D014759:Viral Envelope Proteins / Q000378:metabolism
chemical_list: D015815:Cell Adhesion Molecules; D015696:Gene Products, tat; D015699:HIV Envelope Protein gp120; C000619010:NECTIN1 protein, human; D000075983:Nectins; D014759:Viral Envelope Proteins; C039447:glycoprotein D, Human herpesvirus 1