title: [Sentinel lymph node in pulmonary carcinoma. Molecular study after radioisotope detection].
abstract: BACKGROUND
  Metastatic lymph node affectation is the main prognostic factor in localized lung cancer. Pathological study of the obtained samples even after an adequate lymphadenectomy, present tumoral relapses of 40% of stage I patients after oncological curative surgery. In this paper we have studied micrometastasis in the sentinel lymph node by molecular methods in patients with stage I lung cancer.
  
  
  METHODS
  The sentinel node was marked by injecting peritumorally performed just after performing the thoracotomy with 2 mCi of nanocoloid of albumin (Nanocol®) marked with 99mTc in 0.3 ml. Guided with a Navigator® gammagraphic sensor, we proceeded to its resection. RNA of the tissue was extracted and the presence of genes CEACAM5, PLUNC and CK7 in mRNA was studied.
  
  
  RESULTS
  Twenty nine 29 patients were included. Of the tested genes, CEACAM5 and PLUNC were the ones that showed a high expression in lung tissue. Of the 29 analyzed sentinel lymph nodes, 7 (24%) were positive in the molecular study. A positive sentinel lymph node was found in 4/7 adenocarcinomas and 3/12 squamous-cell tumors. Affected lymph nodes were: station 5 (1/3), station 7 (0/6), station 9 (0/1); station 10 (5/11); station 11 (1/1).
  
  
  CONCLUSIONS
  Detection of sentinel node in patients with stage I lung cancer by marking with radioisotope is a feasible technique. The application of molecular techniques shows the tumoral affectation in cases staged as stage I.
authors.forename: José M
authors.initials: JM
authors.lastname: Galbis Caravajal
authors.forename: Antonio Cremades
authors.initials: AC
authors.lastname: Mira
authors.forename: Ángel Zuñiga
authors.initials: ÁZ
authors.lastname: Cabrera
authors.forename: Miriam Estors
authors.initials: ME
authors.lastname: Guerrero
authors.forename: Ana Tembl
authors.initials: AT
authors.lastname: Ferrairó
authors.forename: Néstor J
authors.initials: NJ
authors.lastname: Martinez Hernandez
authors.forename: Regina Gironés
authors.initials: RG
authors.lastname: Sarrió
authors.forename: Francisco Aparisi
authors.initials: FA
authors.lastname: Aparisi
authors.forename: Cristóbal Gaspar
authors.initials: CG
authors.lastname: Martinez
mesh_terms: D000368:Aged; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000000981:diagnostic imaging* / Q000235:genetics / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals; D021701:Sentinel Lymph Node Biopsy / Q000379:methods*; D013668:Technetium Tc 99m Aggregated Albumin
chemical_list: D019275:Radiopharmaceuticals; D013668:Technetium Tc 99m Aggregated Albumin; C083602:technetium Tc 99m nanocolloid