title: Effect and mechanisms of human Wharton's jelly-derived mesenchymal stem cells on type 1 diabetes in NOD model.
abstract: Type 1 diabetes is an autoimmune disease that results from an inflammatory destruction of β-cells in islets. Mesenchymal stem cells derived from Wharton's jelly (WJ-MSCs) own a peculiar immunomodulatory feature and might reverse the inflammatory destruction and repair the function of β-cells. Sixty NOD mice were divided into four groups, including normal control group, WJ-MSCs prevention group (before onset), WJ-MSCs treatment group (after onset), and diabetic control group. After homologous therapy, onset time of diabetes, levels of fasting plasma glucose (FPG), fed blood glucose and C-peptide, regulation of cytokines, and islet cells were examined and evaluated. After WJ-MSCs infusion, FPG and fed blood glucose in WJ-MSCs treatment group decreased to normal level in 6-8 days and maintained for 6 weeks. Level of fasting C-peptide of these mice was higher compared to diabetic control mice (P=0.027). In WJ-MSCs prevention group, WJ-MSCs played a protective role for 8-week delayed onset of diabetes, and fasting C-peptide in this group was higher compared to the other two diabetic groups (P=0.013, 0.035). Compared with diabetic control group, frequencies of CD4+CD25+Foxp3+ Tregs in WJ-MSCs prevention group and treatment group were higher, while levels of IL-2, IFN-γ, and TNF-α were lower (P<0.001); the degree of insulitis was also depressed, especially for WJ-MSCs prevention group (P<0.05). Infusion of WJ-MSCs could aid in T1DM through regulation of the autoimmunity and recovery of islet β-cells no matter before or after onset of T1DM. WJ-MSCs might be an effective method for T1DM.
authors.affiliation: Stem Cell Research Center, The Affiliated Hospital of Medical College, Qingdao University, No. 16, Jiangsu Road, Qingdao, 266003, China.
authors.forename: Jianxia
authors.initials: J
authors.lastname: Hu
authors.forename: Yangang
authors.initials: Y
authors.lastname: Wang
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Luan
authors.initials: L
authors.lastname: Wang
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Yu
authors.forename: Ruixia
authors.initials: R
authors.lastname: Sun
authors.forename: Zhongchao
authors.initials: Z
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Gao
authors.forename: Zhengju
authors.initials: Z
authors.lastname: Fu
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Zhao
authors.forename: Shengli
authors.initials: S
authors.lastname: Yan
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002096:C-Peptide / Q000378:metabolism; D002454:Cell Differentiation; D016207:Cytokines / Q000097:blood; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics / Q000517:prevention & control*; D005260:Female; D007515:Islets of Langerhans / Q000378:metabolism; D018655:Lymphocyte Count; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051379:Mice; D016688:Mice, Inbred NOD; D013154:Spleen / Q000166:cytology; D059631:Wharton Jelly / Q000166:cytology*
chemical_list: D001786:Blood Glucose; D002096:C-Peptide; D016207:Cytokines