title: Effects of miR-31 on the osteogenesis of human mesenchymal stem cells.
abstract: Exploring the molecular mechanisms that regulate the osteogenesis of human mesenchymal stem cells (hMSCs) will bring us more efficient methods for improving the treatment of bone-related diseases. In this study, we analyzed the effects of miR-31 on the osteogenesis of hMSCs. The overexpression of miR-31 repressed the osteogenesis of hMSCs, whereas the downregulation enhanced this process. SATB2 was testified to be a direct target of miR-31, and its effects on the osteogenesis were also described. Most importantly, the knockdown of SATB2 attenuated miR-31's osteogenic effects. Taken together, our findings suggest that miR-31 regulates the osteogenesis of hMSCs by targeting SATB2.
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Xie
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Zi
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Bi
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Huifang
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Yefei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address: guping2009@hotmail.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Gu
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address: fanxq@sh163.net.
authors.forename: Xianqun
authors.initials: X
authors.lastname: Fan
mesh_terms: D020413:3' Untranslated Regions; D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D055785:Gene Knockdown Techniques; D006801:Humans; D036961:Matrix Attachment Region Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000502:physiology; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism*; D010012:Osteogenesis / Q000235:genetics* / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D014157:Transcription Factors / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000502:physiology
chemical_list: D020413:3' Untranslated Regions; C534612:MIRN31 microRNA, human; D036961:Matrix Attachment Region Binding Proteins; D035683:MicroRNAs; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C521297:SATB2 protein, human; D014157:Transcription Factors
keywords: Human mesenchymal stem cells (hMSCs); Osteogenesis; SATB2; miR-31