title: Isolation, characterization and antigenic cross-reactivities of the major hemorrhagin from Cryptelytrops purpureomaculatus venom.
abstract: The major hemorrhagin from C. purpureomaculatus (mangrove pit viper) venom was purified to homogeneity and termed Maculatoxin. Maculatoxin has a molecular weight of 38 kDa as determined by SDS-PAGE. It is an acidic protein (pI= 4.2) and exhibited proteolytic and hemorrhagic activities (MHD10 = 0.84 microg in mice) but was not lethal to mice at a dose of 1 microg/g. The hemorrhagic activity of Maculatoxin was completely inactivated by EDTA and partially inhibited by ATP and citrate. The N-terminal sequence of Maculatoxin (TPEQQRFPPTYIDLGIFVDHGMYAT) shares a significant degree of homology with the metalloprotease domain of other venom hemorrhagins. Indirect ELISA showed anti-Maculatoxin cross reacted with protein components of many snake venoms. In the double-sandwich ELISA, however, anti-Maculatoxin cross-reacted only with venoms of certain species of the Trimeresurus (Asia lance-head viper) complex, and the results support the recent proposed taxonomy changes concerning the Trimeresurus complex.
authors.affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. fungshinyee@gmail.com
authors.forename: Shin Yee
authors.initials: SY
authors.lastname: Fung
authors.affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Nget Hong
authors.initials: NH
authors.lastname: Tan
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D003429:Cross Reactions / Q000276:immunology; D010450:Endopeptidases / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D051379:Mice; D008970:Molecular Weight; D012910:Snake Venoms / Q000235:genetics / Q000276:immunology*; D013045:Species Specificity; D017841:Trimeresurus / Q000276:immunology* / Q000502:physiology
chemical_list: D012910:Snake Venoms; D010450:Endopeptidases; C041605:hemorrhagic proteinase IV