title: Pedobacter jejuensis sp. nov., isolated from soil of a pine grove, and emended description of the genus Pedobacter.
abstract: A Gram-stain-negative, strictly aerobic, non-motile, rod-shaped bacterial strain, THG-DR3T, was isolated from soil of a pine grove in Jeju island, Republic of Korea. Strain THG-DR3T grew optimally at 20-25 °C, at pH 7.0-8.0 and in R2A broth without added NaCl. 16S rRNA gene sequence analysis indicated that strain THG-DR3T belongs to the genus Pedobacter. The closest relatives based on 16S rRNA gene sequence similarity were Pedobacter aquatilis AR107T (98.0%), Pedobacter alluvionis NWER-II11T (97.7%), Pedobacter borealis G-1T (97.7%) and Pedobacter ginsengiterrae DCY49T (97.4%). The G+C content of the genomic DNA of strain THG-DR3T was 47.5 mol%. Levels of DNA-DNA relatedness between strain THG-DR3T and its closest phylogenetic neighbours were below 44.7%. The presence of menaquinone 7 and of iso-C15:0 and summed feature 3 (comprising C16:1ω7c and/or C16:1ω6c) as the major fatty acids supported the affiliation of strain THG-DR3T to the genus Pedobacter. The polar lipids of strain THG-DR3T consisted of phosphatidylethanolamine, three unidentified aminophospholipids, five unidentified aminolipids and three unidentified lipids. The results of physiological and biochemical tests allowed the differentiation of strain THG-DR3T from recognized species of the genus Pedobacter. Strain THG-DR3T (=KACC 17172T=JCM 18824T) is proposed as the type strain of a novel species of the genus Pedobacter, Pedobacter jejuensis sp. nov. An emended description of the genus Pedobacter is also proposed.
authors.affiliation: Department of Marine Biotechnology, Anyang University, Incheon 417-833, Republic of Korea.
authors.forename: MooChang
authors.initials: M
authors.lastname: Kook
authors.affiliation: Department of Oriental Medicinal Material & Processing College of Life Science, Kyung Hee University, Yongin, Kyunggi 446-701, Republic of Korea.
authors.forename: YongJin
authors.initials: Y
authors.lastname: Park
authors.affiliation: Department of Oriental Medicinal Material & Processing College of Life Science, Kyung Hee University, Yongin, Kyunggi 446-701, Republic of Korea.
authors.forename: Tae-Hoo
authors.initials: TH
authors.lastname: Yi
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D060485:Pedobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D028223:Pinus / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C062629:menaquinone 7