title: A fluorescent off-on NBD-probe for F(-) sensing: theoretical validation and experimental studies.
abstract: The design, synthesis and fluoride sensing ability of a 7-nitro-2,1,3-benzoxadiazole (NBD) based chemodosimeter is reported. Theoretical calculations were used to design a more applicable off-on response, by choosing NBD as the accurate fluorophore. Reaction of the NBD-probe with 300 equivalents of tetrabutyl ammonium fluoride (TBAF) exhibited a response time of 80 minutes and the reaction was selective to F(-) and sensing of the ion was marked by a 110-fold enhancement of green fluorescence. The off-on fluorescence characteristics of the probe enabled its application in live-cell imaging of intracellular F(-) ions.
authors.affiliation: Department of Chemistry, Indian Institute of Science Education and Research, Pune, India. ptalukdar@iiserpune.ac.in.
authors.forename: Arundhati
authors.initials: A
authors.lastname: Roy
authors.forename: Avdhoot
authors.initials: A
authors.lastname: Datar
authors.forename: Dnyaneshwar
authors.initials: D
authors.lastname: Kand
authors.forename: Tanmoy
authors.initials: T
authors.lastname: Saha
authors.forename: Pinaki
authors.initials: P
authors.lastname: Talukdar
mesh_terms: D001393:Azoles / Q000138:chemical synthesis / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D005461:Fluorine / Q000032:analysis*; D015394:Molecular Structure; D009578:Nitrobenzenes / Q000138:chemical synthesis / Q000737:chemistry*; D011789:Quantum Theory
chemical_list: C510797:7-nitrobenz-2-oxa-1,3-diazol-4-yl; D001393:Azoles; D005456:Fluorescent Dyes; D009578:Nitrobenzenes; D005461:Fluorine