title: Establishment of successively transplantable rabbit VX2 cancer cells that express enhanced green fluorescent protein.
abstract: Morphological detection of cancer cells in the rabbit VX2 allograft transplantation model is often difficult in a certain region such as serosal cavity where reactive mesothelial cells mimic cancer cells and both cells share common markers such as cytokeratins. Therefore, tagging VX2 cells with a specific and sensitive marker that easily distinguishes them from other cells would be advantageous. Thus, we tried to establish a successively transplantable, enhanced green fluorescent protein (EGFP)-expressing VX2 model. Cancer cells obtained from a conventional VX2-bearing rabbit were cultured in vitro and transfected with an EGFP-encoding vector, and then successively transplanted in Healthy Japanese White rabbits (HJWRs) (n = 8). Besides, conventional VX2 cells were transplanted in other HJWRs (n = 8). Clinicopathological comparison analyses were performed between the two groups. The success rate of transplantation was 100% for both groups. The sensitivity and specificity of EGFP for immunohistochemical detection of VX2 cells were 84.3 and 100%, respectively. No significant differences in cancer cell morphology, tumor size (P = 0.742), Ki-67 labeling index (P = 0.878), or survival rate (P = 0.592) were observed between the two. VX2 cells can be genetically altered, visualized by EGFP, and successively transplanted without significant alteration of morphological and biological properties compared to those of the conventional model.
authors.affiliation: Department of Pathology, Yokohama City University Graduate School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama, Kanagawa, 236-0004, Japan, oshiroh@yokohama-cu.ac.jp.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Oshiro
authors.forename: Hidenobu
authors.initials: H
authors.lastname: Fukumura
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Nagahama
authors.forename: Itaru
authors.initials: I
authors.lastname: Sato
authors.forename: Kei
authors.initials: K
authors.lastname: Sugiura
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Iobe
authors.forename: Emi
authors.initials: E
authors.lastname: Okiyama
authors.forename: Toshitaka
authors.initials: T
authors.lastname: Nagao
authors.forename: Yoji
authors.initials: Y
authors.lastname: Nagashima
authors.forename: Ichiro
authors.initials: I
authors.lastname: Aoki
authors.forename: Shoji
authors.initials: S
authors.lastname: Yamanaka
authors.forename: Ayumi
authors.initials: A
authors.lastname: Murakami
authors.forename: Jiro
authors.initials: J
authors.lastname: Maegawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Chishima
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ichikawa
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nagai
authors.forename: Masaharu
authors.initials: M
authors.lastname: Nomura
authors.forename: Kenichi
authors.initials: K
authors.lastname: Ohashi
authors.forename: Koji
authors.initials: K
authors.lastname: Okudela
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D007150:Immunohistochemistry; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D009368:Neoplasm Transplantation / Q000379:methods*; D009374:Neoplasms, Experimental / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D011817:Rabbits; D016019:Survival Analysis; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000637:transplantation*
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins