title: Multicolor labeling in developmental gene regulatory network analysis.
abstract: The sea urchin embryo is an important model system for developmental gene regulatory network (GRN) analysis. This chapter describes the use of multicolor fluorescent in situ hybridization (FISH) as well as a combination of FISH and immunohistochemistry in sea urchin embryonic GRN studies. The methods presented here can be applied to a variety of experimental settings where accurate spatial resolution of multiple gene products is required for constructing a developmental GRN.
authors.affiliation: National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA.
authors.forename: Aditya J
authors.initials: AJ
authors.lastname: Sethi
authors.forename: Robert C
authors.initials: RC
authors.lastname: Angerer
authors.forename: Lynne M
authors.initials: LM
authors.lastname: Angerer
mesh_terms: D000818:Animals; D036703:Blastula / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D018507:Gene Expression Regulation, Developmental; D053263:Gene Regulatory Networks; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D012617:Sea Urchins / Q000235:genetics* / Q000378:metabolism; D013194:Staining and Labeling; D016707:Tissue Fixation
chemical_list: D005456:Fluorescent Dyes