title: Monitoring the proteostasis function of the Saccharomyces cerevisiae metacaspase Yca1.
abstract: The functional versatility of metacaspase proteases has been established by reports of their involvement in non-apoptotic cellular processes, in addition to their canonical role in apoptosis/programmed cell death. While the budding yeast metacaspase Yca1 has been well characterized for its role in cell death regulation, more recent examinations suggest that the protease may be involved in key processes that increase survival and fitness. More specifically, examinations suggest that Yca1 is central to maintaining cellular proteostasis as it interacts with major components involved in protein biosynthesis and functions to limit aggregate deposition. Here, we describe the methods utilized to analyze the role Yca1 in proteostasis.
authors.affiliation: Regenerative Medicine Program, Sprott Centre for Stem Cell Research, Ottawa Hospital Research Institute, The Ottawa Hospital, Ottawa, Ontario, Canada.
authors.forename: Amit
authors.initials: A
authors.lastname: Shrestha
authors.forename: Robin E C
authors.initials: RE
authors.lastname: Lee
authors.forename: Lynn A
authors.initials: LA
authors.lastname: Megeney
mesh_terms: D017209:Apoptosis / Q000235:genetics; D020169:Caspases / Q000096:biosynthesis* / Q000235:genetics; D008967:Molecular Biology / Q000379:methods*; D014176:Protein Biosynthesis / Q000235:genetics; D057165:Proteostasis Deficiencies / Q000201:enzymology / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D020169:Caspases; C456272:MCA1 protein, S cerevisiae