title: Improved establishment of embryonic stem (ES) cell lines from the Chinese Kunming mice by hybridization with 129 mice.
abstract: Chinese Kunming mice (Mus musculus Km), widely used as laboratory animals throughout China, remain very refractory for embryonic stem (ES) cell isolation. The present study was aimed to evaluate the effects of hybridization with 129/Sv mice, and culture media containing fetal bovine serum (FBS) or Knockout serum replacement (KSR) on ES cell isolation from Kunming mice. The results demonstrated that ES cells had been effectively isolated from the hybrid embryos of Kunming and 129/Sv mice using all three media containing 15% FBS, 15% KSR and their mixture of 14% KSR and 1% FBS, individually. These isolated ES cells had maintained in vitro undifferentiated for a long time, exhibiting all features specific for mouse ES cells. In addition, the rates of ES cell isolation in the medium containing 14% KSR and 1% FBS, was 46.67% and significantly higher than those in another two media containing only FBS or KSR (p < 0.05). Contrarily, no ES cell line had been established from Kunming mouse inbred embryos using the same protocols. These results suggested that ES cells with long-term self-renewal ability could be efficiently generated from hybrid embryos of Kunming and 129/Sv mice, and a small volume of FBS was necessary to isolate ES cells in the KSR medium when embryos and early ES cells cultured.
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Xinkang Road 46#, Yucheng District, Ya'an 625014, Sichuan, China. yayushumin@163.com.
authors.forename: Shumin
authors.initials: S
authors.lastname: Yu
authors.affiliation: Life Science College, North-West University, Xi'an 710069, Shaanxi, China. xingrongyan2007@126.com.
authors.forename: Xingrong
authors.initials: X
authors.lastname: Yan
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Xinkang Road 46#, Yucheng District, Ya'an 625014, Sichuan, China. liuhuanhd@163.com.
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Liu
authors.affiliation: School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, Sichuan, China. caixin2323@126.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Cai
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Xinkang Road 46#, Yucheng District, Ya'an 625014, Sichuan, China. suizhongcao@126.com.
authors.forename: Suizhong
authors.initials: S
authors.lastname: Cao
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Xinkang Road 46#, Yucheng District, Ya'an 625014, Sichuan, China. shenlh@sicau.edu.cn.
authors.forename: Liuhong
authors.initials: L
authors.lastname: Shen
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Xinkang Road 46#, Yucheng District, Ya'an 625014, Sichuan, China. zhihua_ren@126.com.
authors.forename: Zhicai
authors.initials: Z
authors.lastname: Zuo
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Xinkang Road 46#, Yucheng District, Ya'an 625014, Sichuan, China. dengjl213@126.com.
authors.forename: Junliang
authors.initials: J
authors.lastname: Deng
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Xinkang Road 46#, Yucheng District, Ya'an 625014, Sichuan, China. mxp886@sina.com.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Ma
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Xinkang Road 46#, Yucheng District, Ya'an 625014, Sichuan, China. wangyayang@126.com.
authors.forename: Ya
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Xinkang Road 46#, Yucheng District, Ya'an 625014, Sichuan, China. zhihua_ren@126.com.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Ren
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003433:Crosses, Genetic; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D058732:Embryoid Bodies / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D005838:Genotype; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006823:Hybrid Vigor / Q000235:genetics*; D006824:Hybridization, Genetic; D007150:Immunohistochemistry; D059785:Karyotype; D008297:Male; D057507:Mice, 129 Strain; D008819:Mice, Nude; D018895:Microsatellite Repeats / Q000235:genetics; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D003470:Culture Media; D018398:Homeodomain Proteins; D000071317:Nanog Homeobox Protein; C475540:Nanog protein, mouse; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse