title: Yeast two-hybrid screen.
abstract: Yeast two-hybrid is a method for screening large numbers of gene products (encoded by cDNA libraries) for their ability to interact with a protein of interest. This system can also be used for characterizing and manipulating candidate protein: protein interactions. Interactions between proteins are monitored by the growth of yeast plated on selective media.
authors.affiliation: Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: lmakuch1@jhmi.edu.
authors.forename: Lauren
authors.initials: L
authors.lastname: Makuch
mesh_terms: D003470:Culture Media; D004926:Escherichia coli; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000254:growth & development*; D020798:Two-Hybrid System Techniques
chemical_list: D003470:Culture Media
keywords: Activation domain (AD) fusion protein; Back-transformation of yeast; Cloning and transformation methods; Confirmation of positive interactors; DNA-binding domain (DB) fusion protein; Electroporation of E. coli; Plasmid rescue; Self-activation of pDBLeu-X (and pPC86-Y); Small-scale yeast transformation; Yeast two-hybrid screen; pDBLeu-X