title: Do adipose tissue-derived mesenchymal stem cells ameliorate Parkinson's disease in rat model?
abstract: Parkinson's disease (PD) is a common neurodegenerative disorder in middle-aged and elderly people. This study aimed to elucidate the role of mesenchymal stem cells (MSCs) in management of PD in ovariectomized rat model. MSCs were excised from adipose tissue of both the omentum and the inguinal fat pad of male rats, grown, and propagated in culture; then characterized morphologically; and by the detection of surface markers gene expression. In this study, 40 ovariectomized animals were classified into 5 groups; group 1 was ovariectomized control, groups 2 to 5 were subcutaneously administered with rotenone for 14 days after 1 month of ovariectomy for induction of PD. Group 2 was left untreated; groups 3, 4, and 5 were treated with Sinemet(®), Cerebrolysin(®), and a single dose of adipose tissue-derived MSCs (ADMSCs), respectively. Y-chromosome gene (sry) was assessed by polymerase chain reaction (PCR) in brain tissue of the female rats. Serum transforming growth factor β (TGF-β), monocyte chemoattractant protein 1 (MCP-1), and brain-derived neurotrophic factor (BDNF) levels were assayed using enzyme-linked immunosorbent assay technique. Brain dopamine level was assayed fluorometrically, while brain tyrosine hydroxylase (TH) gene expression was detected by semiquantitative real-time PCR. The PD group showed significant increase in serum TGF-β and MCP-1 levels associated with significant decrease in serum BDNF, brain dopamine, and brain TH gene expression levels. In contrast, all treatments produce significant decrease in serum TGF-β and MCP-1 levels in concomitant with significant increase in serum BDNF, brain dopamine, and brain TH gene expression levels. In conclusion, the observed improvements in the studied biomarkers due to ADMSCs infusion might be attributed to their immunomodulatory, anti-inflammatory, and neurotrophic effects.
authors.affiliation: Department of Hormones, Medical Research Division, National Research Centre, Cairo, Egypt hanaaomr@yahoo.com.
authors.forename: Hh
authors.initials: H
authors.lastname: Ahmed
authors.affiliation: Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt.
authors.forename: Am
authors.initials: A
authors.lastname: Salem
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors.forename: Hm
authors.initials: H
authors.lastname: Atta
authors.affiliation: Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt.
authors.forename: Ma
authors.initials: M
authors.lastname: Ghazy
authors.affiliation: Department of Hormones, Medical Research Division, National Research Centre, Cairo, Egypt.
authors.forename: Ha
authors.initials: H
authors.lastname: Aglan
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D015703:Antigens, CD / Q000235:genetics; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D019208:Brain-Derived Neurotrophic Factor / Q000097:blood; D002230:Carbidopa / Q000494:pharmacology; D002454:Cell Differentiation; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000097:blood; D004195:Disease Models, Animal; D004338:Drug Combinations; D005260:Female; D015870:Gene Expression; D024282:Genes, sry; D007980:Levodopa / Q000494:pharmacology; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D010052:Ovariectomy; D010300:Parkinson Disease / Q000097:blood / Q000209:etiology / Q000378:metabolism / Q000628:therapy*; D017207:Rats, Sprague-Dawley; D012402:Rotenone; D016212:Transforming Growth Factor beta / Q000097:blood; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D000596:Amino Acids; D015703:Antigens, CD; D019208:Brain-Derived Neurotrophic Factor; C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D004338:Drug Combinations; D016212:Transforming Growth Factor beta; C009265:carbidopa, levodopa drug combination; D012402:Rotenone; C006952:cerebrolysin; D007980:Levodopa; D014446:Tyrosine 3-Monooxygenase; D002230:Carbidopa
keywords: Parkinson's disease; adipose tissue-derived mesenchymal stem cells; anti-inflammatory activity; immunomodulatory effect; neurotrophic capacity