title: Molecular cloning and mRNA expression profile of sucrose transporter gene BnSUT1C from Brassica napus L.
abstract: The genomic and cDNA sequences of BnSUT1C were isolated from B. napus. Combination of cDNA and genomic DNA sequences revealed that the BnSUT1C gene contained three exons and two introns. The cDNA encodes a protein of 513 amino acids with a calculated molecular mass of 54.7 kDa and an isoelectric point of 9.12. It exhibits typical features of sucrose transporter with 12 trans-membranes spanning domains. BnSUT1C showed highly homologous with AtSUC1 and AtSUC5. A histidine residue, which is conserved across all functional sucrose transporter proteins in higher plants, is located at position 66 of the BnSUT1C. Two putative pollen-specific cis-elements, AGAAA and GTGA motifs, are located in 5'-upstream of BnSUT1C. The spatial and temporal expression patterns carried out by semi-quantitative RT-PCR and Real-Time PCR, which indicated that BnSUT1C predominantly expressed in later developmental stages of anther, as tapetal cells began to shrink and collapse. BnSUT1C could mediate the uptake of sucrose in the pollen and retrieval of tapetal degenerated products during pollen maturation.
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P.R. China.
authors.forename: FuPeng
authors.initials: F
authors.lastname: Li
authors.affiliation: Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Science, Wanning 571533, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Yan
authors.affiliation: Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Science, Wanning 571533, P.R. China.
authors.forename: Jianxiong
authors.initials: J
authors.lastname: Lai
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P.R. China.
authors.forename: Chaozhi
authors.initials: C
authors.lastname: Ma
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P.R. China.
authors.forename: Mayank
authors.initials: M
authors.lastname: Gautam
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P.R. China.
authors.forename: Tingdong
authors.initials: T
authors.lastname: Fu
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D001483:Base Sequence; D029688:Brassica napus / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D026901:Membrane Transport Proteins / Q000235:genetics / Q000302:isolation & purification*; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D059467:Transcriptome
chemical_list: D018076:DNA, Complementary; D026901:Membrane Transport Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; C078729:sucrose transport protein, plant; D004247:DNA