title: Comparison of the anti-amyloidogenic effect of O-mannosylation, O-galactosylation, and O-GalNAc glycosylation.
abstract: Our aim was to explore the effects of functional groups at carbon-2 (C2) of a sugar on the conformational properties of the peptide backbone. Three monosaccharides, mannose, galactose, and N-acetylgalactosamine (GalNAc), were added separately to the serine side-chain of a hamster prion peptide because it is a sensitive model for comparing the effect of protein modification on the conformational properties of the polypeptide chain. In buffer, this prion peptide goes through a gradual coil-to-β structural conversion and forms amyloid fibrils slowly during incubation. Our results showed that a sugar with an N-acetyl amino group in the equatorial configuration (GalNAc) or with a hydroxyl group in the axial configuration (mannose) on C2 had a greater inhibitory effect on the amyloidogenesis of the prion peptide than a sugar with the hydroxyl group in the equatorial configuration (galactose). We suggest that galactosylation has less effect than mannosylation or GalNAc glycosylation on promoting turn formation at the glycosylation site and on inhibition of amyloidogenesis. The anti-amyloidogenic property of mannose implies that protein mannosylation has an anti-aggregation function.
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, No 128, Sec 2, Academia Rd, Nankang, Taipei 11529, Taiwan.
authors.forename: Chen
authors.initials: C
authors.lastname: Lin
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, No 128, Sec 2, Academia Rd, Nankang, Taipei 11529, Taiwan.
authors.forename: Eric H-L
authors.initials: EH
authors.lastname: Chen
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, No 128, Sec 2, Academia Rd, Nankang, Taipei 11529, Taiwan.
authors.forename: Lily Y-L
authors.initials: LY
authors.lastname: Lee
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, No 128, Sec 2, Academia Rd, Nankang, Taipei 11529, Taiwan.
authors.forename: Ruei-Lin
authors.initials: RL
authors.lastname: Hsu
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, No 128, Sec 2, Academia Rd, Nankang, Taipei 11529, Taiwan.
authors.forename: Frederick Y
authors.initials: FY
authors.lastname: Luh
authors.affiliation: Institute of Physics, Academia Sinica, Taipei 11529, Taiwan.
authors.forename: Li-ling
authors.initials: LL
authors.lastname: Yang
authors.affiliation: Institute of Physics, Academia Sinica, Taipei 11529, Taiwan.
authors.forename: Chia-Fu
authors.initials: CF
authors.lastname: Chou
authors.affiliation: Department of Chemistry, National Tsing Hua University, Hsinchu 30013, Taiwan.
authors.forename: Li-De
authors.initials: LD
authors.lastname: Huang
authors.affiliation: Department of Chemistry, National Tsing Hua University, Hsinchu 30013, Taiwan.
authors.forename: Chun-Cheng
authors.initials: CC
authors.lastname: Lin
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, No 128, Sec 2, Academia Rd, Nankang, Taipei 11529, Taiwan; Institute of Biochemical Sciences, National Taiwan University, Taipei 10617, Taiwan. Electronic address: pyc@gate.sinica.edu.tw.
authors.forename: Rita P-Y
authors.initials: RP
authors.lastname: Chen
mesh_terms: D000116:Acetylgalactosamine / Q000737:chemistry*; D000818:Animals; D002244:Carbon / Q000737:chemistry; D006224:Cricetinae; D005690:Galactose / Q000737:chemistry*; D006031:Glycosylation; D008358:Mannose / Q000737:chemistry*; D009077:Mucins / Q000737:chemistry; D017350:N-Acetylgalactosaminyltransferases / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D011487:Protein Conformation; D012694:Serine / Q000737:chemistry
chemical_list: D009077:Mucins; D010455:Peptides; D012694:Serine; D002244:Carbon; D017350:N-Acetylgalactosaminyltransferases; D000116:Acetylgalactosamine; D008358:Mannose; D005690:Galactose
keywords: Amyloid; GalNAc; Galactosylation; Glycosylation; Mannosylation; Prion