title: A higher frequency of peripheral blood activated B cells in patients with non-traumatic osteonecrosis of the femoral head.
abstract: OBJECTIVE
  B cells play important roles in inflammatory diseases. This study was aimed at examining the frequency of different subsets of B cells in patients with non-traumatic osteonecrosis of the femoral head (NONFH).
  
  
  METHODS
  The percentages of the different subsets of circulating B cells in 28 patients with steroid-related, alcohol-related, or idiopathic NONFH and 10 healthy controls (HC) were examined by flow cytometry. The concentrations of serum C-reactive protein (CRP), fibrinogen (FIB), immunoglobulins, cytokines and blood erythrocyte sedimentation rate (ESR) were measured.
  
  
  RESULTS
  In comparison with those in the HC, significantly higher percentages of CD27-, CD86+, CD95+, and CD27+CD95+CD19+ but lower CD27+CD19+ B cells were detected in the patients. The percentages of CD86+, CD95+, and CD27+CD95+CD19+ B cells in each group of the patients were significantly higher than those in the HC. The levels of serum IL-17A and IFN-γ in steroid group and serum TNF-α in alcoholic group were significantly higher than those in the HC. The percentages of CD86+CD19+ B cells were positively associated with the degrees of femoral head collapse in both steroid and alcoholic groups of patients and the levels of serum TNF-α were positively associated with the degrees of femoral head collapse in the alcoholic NONFH patients.
  
  
  CONCLUSIONS
  These data suggest a higher frequency of CD86+CD19+ activated B cells and elevated levels of serum TNF-α may be associated with the development of NONFH.
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital, Jilin University, Changchun 130032, China.
authors.forename: Haiyu
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital, Jilin University, Changchun 130032, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital, Jilin University, Changchun 130032, China.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital, Jilin University, Changchun 130032, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital, Jilin University, Changchun 130032, China. Electronic address: shanyuxingvip@163.com.
authors.forename: Yuxing
authors.initials: Y
authors.lastname: Shan
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital, Jilin University, Changchun 130032, China. Electronic address: yanfangjiang@hotmail.com.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000328:Adult; D000368:Aged; D000437:Alcoholism / Q000097:blood / Q000453:epidemiology / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D001799:Blood Sedimentation; D002097:C-Reactive Protein / Q000378:metabolism; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D016207:Cytokines / Q000097:blood / Q000276:immunology; D005260:Female; D005271:Femur Head Necrosis / Q000097:blood / Q000453:epidemiology / Q000276:immunology*; D005340:Fibrinogen; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology; D008297:Male; D008875:Middle Aged
chemical_list: D015703:Antigens, CD; D016207:Cytokines; D007136:Immunoglobulins; D005340:Fibrinogen; D002097:C-Reactive Protein
keywords: B cells; CD86; CD95; NONFH; TNF-α