title: Molecularly imprinted polymer based electrochemical detection of L-cysteine at carbon paste electrode.
abstract: A methacrylic acid (MAA) based molecularly imprinted polymer (MIP) modified carbon paste electrode (CPE) was developed for electrochemical detection of L-cysteine (Cys). Characterisation of MIP was done with FTIR and the modified electrode with cyclic voltammetry (CV) and differential pulse voltammetry (DPV). CV, DPV and impedance analysis demonstrated that the modified electrode is responsive towards the target molecule. The optimum percentage composition of MIP for MIP/CPE and the effect of pH towards the electrode response for Cys were studied. The detection of Cys in the range of 2×10(-8) to 18×10(-8)M at MIP/CPE was monitored by DPV with a limit of detection of 9.6nM and R(2) of 0.9974. Also, various physiological interferents such as ascorbic acid, L-tryptophan, D-glucose, D-cysteine and L-cysteine were found to have little effect on DPV response at MIP/CPE. The utility of the electrode was proved by the effective detection of Cys from tap water and human blood plasma samples with reproducible results.
authors.affiliation: Department of Chemistry, National Institute of Technology, Tiruchirappalli, Tamil Nadu, 620015, India. Electronic address: aswinikk@ymail.com.
authors.forename: K K
authors.initials: KK
authors.lastname: Aswini
authors.affiliation: Department of Chemistry, National Institute of Technology, Tiruchirappalli, Tamil Nadu, 620015, India. Electronic address: vinumohan756@gmail.com.
authors.forename: A M
authors.initials: AM
authors.lastname: Vinu Mohan
authors.affiliation: Department of Chemistry, National Institute of Technology, Tiruchirappalli, Tamil Nadu, 620015, India. Electronic address: vmbiju@ymail.com.
authors.forename: V M
authors.initials: VM
authors.lastname: Biju
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D002244:Carbon / Q000737:chemistry*; D003545:Cysteine / Q000032:analysis* / Q000097:blood; D055664:Electrochemical Techniques; D004566:Electrodes; D005618:Fresh Water / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008689:Methacrylates / Q000737:chemistry; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D008689:Methacrylates; D011108:Polymers; C008384:methacrylic acid; D002244:Carbon; D003545:Cysteine
keywords: Carbon paste electrode; Ferro-ferricyanide; L-cysteine; Molecular imprinted polymer