title: Antitumor activity of the protein and small molecule component fractions from Agrocybe aegerita through enhancement of cytokine production.
abstract: A water soluble extract from the medicinal mushroom Agrocybe aegerita has been shown to stimulate splenocyte proliferation, cytotoxic activity, and tumor rejection effect in tumor-bearing mouse models. In the present study, the crude extract was separated into a protein component fraction (Yp), mainly containing lectins and serine proteinase, and a small molecule component fraction (Ys), mainly containing triethylene glycol, α-bisabolol, n-hexadecanoic acid, and so on. The antitumor activity of the fractions was investigated in a tumor-bearing BALB/c mouse model. Repeat administration of Yp and Ys significantly inhibited tumor growth (P<.001), but little toxicity was observed. Moreover, the protein fraction Yp performed better than Ys in both antitumor and lifespan-prolonging activity. The cytokine expression levels in serum and splenocytes from extract-treated mice were selectively screened by enzyme-linked immunosorbent assay and quantitative real-time polymerase chain reaction, and the results showed that Yp upregulated the mRNA level of Th2 cytokine interleukin-10 (P<.01), and Ys increased the mRNA level of granulocyte-macrophage colony-stimulating factor (P<.01) and anti-inflammatory cytokine transforming growth factor-β (P<.01). All these data suggest that Yp and Ys can inhibit tumor growth via different mechanisms, which promotes the understanding of antitumor properties of medicinal fungi.
authors.affiliation: 1 Department of Clinical Immunology, Guangdong Medical College , Dongguan, China .
authors.forename: Yi
authors.initials: Y
authors.lastname: Liang
authors.forename: Hong-Hong
authors.initials: HH
authors.lastname: Liu
authors.forename: Yi-Jie
authors.initials: YJ
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
mesh_terms: D055362:Agrocybe / Q000737:chemistry*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000378:metabolism; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009369:Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D010940:Plant Proteins / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry*; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism; D014675:Vegetables / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D016207:Cytokines; D010936:Plant Extracts; D010940:Plant Proteins; D053773:Transforming Growth Factor beta1; D016753:Interleukin-10; D016178:Granulocyte-Macrophage Colony-Stimulating Factor