title: In vitro evaluation of the antiviral activity of the synthetic epigallocatechin gallate analog-epigallocatechin gallate (EGCG) palmitate against porcine reproductive and respiratory syndrome virus.
abstract: In this study, epigallocatechin gallate (EGCG) palmitate was synthesized and its anti-porcine reproductive and respiratory syndrome virus (PRRSV) activity was studied. Specifically, EGCG palmitate was evaluated for its ability to inhibit PRRSV infection in MARC-145 cells when administered as pre-, post-, or co-treatment. EGCG and ribavirin were used as controls. The results showed that a 50% cytotoxic concentration (CC50) of EGCG, EGCG palmitate, and ribavirin was achieved at 2,359.71, 431.42, and 94.06 μM, respectively. All three drugs inhibited PRRSV in a dose-dependent manner regardless of the treatment protocol. EGCG palmitate exhibited higher cytotoxicity than EGCG, but lower cytotoxicity than ribavirin. EGCG palmitate anti-PRRSV activity was significantly higher than that of EGCG and ribavirin, both as pre-treatment and post-treatment. Under the former conditions and a tissue culture infectious dose of 10 and 100, the selectivity index (SI) of EGCG palmitate in the inhibition of PRRSV was 3.8 and 2.9 times higher than that of ribavirin when administered as a pre-treatment, while the SI of EGCG palmitate in the inhibition of PRRSV was 3.0 and 1.9 times higher than ribavirin when administered as a post-treatment. Therefore, EGCG palmitate is potentially effective as an anti-PRRSV agent and thus of interest to the pharmaceutical industry.
authors.affiliation: State Engineering Laboratory for Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, China. zcjsj@163.com.
authors.forename: Chunjian
authors.initials: C
authors.lastname: Zhao
authors.affiliation: State Engineering Laboratory for Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, China. woshiliushuaihua@163.com.
authors.forename: Shuaihua
authors.initials: S
authors.lastname: Liu
authors.affiliation: State Engineering Laboratory for Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, China. nefujane@aliyun.com.
authors.forename: Chunying
authors.initials: C
authors.lastname: Li
authors.affiliation: State Engineering Laboratory for Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, China. ylnefu@163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: State Engineering Laboratory for Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, China. zuyuangang@yahoo.com.cn.
authors.forename: Yuangang
authors.initials: Y
authors.lastname: Zu
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000138:chemical synthesis / Q000494:pharmacology* / Q000633:toxicity; D002392:Catechin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology / Q000633:toxicity; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003588:Cytopathogenic Effect, Viral; D050296:Microbial Viability / Q000187:drug effects; D010168:Palmitates / Q000138:chemical synthesis / Q000494:pharmacology* / Q000633:toxicity; D019316:Porcine respiratory and reproductive syndrome virus / Q000187:drug effects* / Q000502:physiology*; D013552:Swine
chemical_list: D000998:Antiviral Agents; D010168:Palmitates; D002392:Catechin; C045651:epigallocatechin gallate