title: 3D microscopic imaging and evaluation of tubular tissue architecture.
abstract: 3D microscopy and image analysis provide reliable measurements of length, branching, density, tortuosity and orientation of tubular structures in biological samples. We present a survey of methods for analysis of large samples by measurement of local differences in geometrical characteristics. The methods are demonstrated on the structure of the capillary bed in a rat brain.
authors.affiliation: Department of Biomathematics, Institute of Physiology Academy of Sciences of the Czech Republic, Prague, Czech Republic. janacek@biomed.cas.cz.
authors.forename: J
authors.initials: J
authors.lastname: Janáček
authors.forename: M
authors.initials: M
authors.lastname: Čapek
authors.forename: J
authors.initials: J
authors.lastname: Michálek
authors.forename: P
authors.initials: P
authors.lastname: Karen
authors.forename: L
authors.initials: L
authors.lastname: Kubínová
mesh_terms: D000818:Animals; D002196:Capillaries / Q000166:cytology*; D002536:Cerebral Arteries / Q000166:cytology*; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D051381:Rats