title: Gracilaria bursa-pastoris (Gmelin) Silva extract attenuates ultraviolet B radiation-induced oxidative stress in human keratinocytes.
abstract: The purpose of this study was to assess the protective effects of an ethanol extract derived from the red alga Gracilaria bursa-pastoris (Gmelin) Silva (GBE) on ultraviolet B (UVB)-irradiated human HaCaT keratinocytes. GBE exhibited scavenging activity against intracellular reactive oxygen species that were induced by either hydrogen peroxide or UVB radiation. In addition, both the superoxide anion and the hydroxyl radical were scavenged by GBE in cell-free systems. GBE absorbed light in the UVB range (280-320 nm) of the electromagnetic spectrum and lessened the extent of UVB-induced oxidative damage to cellular lipids, proteins, and DNA. Finally, GBE-treated keratinocytes showed a reduction in UVB-induced apoptosis, as exemplified by fewer apoptotic bodies. These results suggest that GBE exerts cytoprotective actions against UVB-stimulated oxidative stress by scavenging ROS and absorbing UVB rays, thereby attenuating injury to cellular constituents and preventing cell death.
authors.affiliation: School of Medicine, Jeju National University, Jeju, Republic of Korea.
authors.forename: M J
authors.initials: MJ
authors.lastname: Piao
authors.affiliation: School of Medicine, Jeju National University, Jeju, Republic of Korea.
authors.forename: K C
authors.initials: KC
authors.lastname: Kim
authors.affiliation: School of Medicine, Jeju National University, Jeju, Republic of Korea.
authors.forename: J
authors.initials: J
authors.lastname: Zheng
authors.affiliation: School of Medicine, Jeju National University, Jeju, Republic of Korea.
authors.forename: C W
authors.initials: CW
authors.lastname: Yao
authors.affiliation: School of Medicine, Jeju National University, Jeju, Republic of Korea.
authors.forename: J W
authors.initials: JW
authors.lastname: Cha
authors.affiliation: School of Medicine, Jeju National University, Jeju, Republic of Korea.
authors.forename: H K
authors.initials: HK
authors.lastname: Kang
authors.affiliation: School of Medicine, Jeju National University, Jeju, Republic of Korea.
authors.forename: E S
authors.initials: ES
authors.lastname: Yoo
authors.affiliation: School of Medicine, Jeju National University, Jeju, Republic of Korea.
authors.forename: Y S
authors.initials: YS
authors.lastname: Koh
authors.affiliation: Jeju Biodiversity Research Institute, Jeju Technopark, Jeju, Republic of Korea.
authors.forename: M H
authors.initials: MH
authors.lastname: Ko
authors.affiliation: Department of Chemistry, College of Natural Sciences, Jeju National University, Jeju, Republic of Korea.
authors.forename: N H
authors.initials: NH
authors.lastname: Lee
authors.affiliation: School of Medicine, Jeju National University, Jeju, Republic of Korea.
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Hyun
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D053938:DNA Fragmentation / Q000187:drug effects / Q000528:radiation effects; D044724:Gracilaria; D006801:Humans; D017665:Hydroxyl Radical / Q000378:metabolism; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects*; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D050050:Protein Carbonylation / Q000187:drug effects / Q000528:radiation effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D010936:Plant Extracts; D017382:Reactive Oxygen Species; D013481:Superoxides; D017665:Hydroxyl Radical