title: Equipment and preliminary results for orthogonal pressurized planar electrochromatography.
abstract: We report combination of overpressured layer chromatography (OPLC) and pressurized planar electrochromatography (PPEC) techniques into a single technique in which both OPLC and PPEC processes proceed simultaneously and orthogonally. The separation process with this new technique is performed in adsorbent layer of a chromatographic plate, which is equipped with special sealing margin on its whole periphery and closed under pressure in special chamber. We have named this separation technique as orthogonal pressurized planar electrochromatography (OPPEC). Examples of analytical and micropreparative (continuous) OPPEC separations are demonstrated.
authors.affiliation: Department of Physical Chemistry, Chair of Chemistry, Medical University, Lublin, Poland. Electronic address: tadeusz.dzido@umlub.pl.
authors.forename: Tadeusz H
authors.initials: TH
authors.lastname: Dzido
authors.affiliation: Department of Physical Chemistry, Chair of Chemistry, Medical University, Lublin, Poland. Electronic address: eryk.lopaciuk@umlub.pl.
authors.forename: Eryk
authors.initials: E
authors.lastname: Lopaciuk
authors.affiliation: Department of Cosmetology, Faculty of Physical Education and Sport in Biala Podlaska, Jozef Pilsudski University of Physical Education, Warsaw, Poland.
authors.forename: Paweł W
authors.initials: PW
authors.lastname: Płocharz
authors.affiliation: Department of Physical Chemistry, Chair of Chemistry, Medical University, Lublin, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Chomicki
authors.affiliation: Department of Physical Chemistry, Chair of Chemistry, Medical University, Lublin, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Zembrzycka
authors.affiliation: Chair of Environmental Chemistry and Ecotoxicology, University of Bayreuth, Germany.
authors.forename: Hartmut
authors.initials: H
authors.lastname: Frank
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods*; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods*
keywords: Continuous two-dimensional separation; Electrochromatography; Electrophoresis; Liquid chromatography; Orthogonal pressurized planar electrochromatography; Two dimensional separation