title: [Comparison of two kinds of enzyme-linked immunosorbnent assay for detection of anti-hepatitis C].
abstract: OBJECTIVE
  To compare the difference of sensitivity and specificity on the results of the same samples determined by indirect ELISA and sandwich ELISA.
  
  
  METHODS
  A 51 anti-HCV positive serum samples obtained from donors were screened by two indirect ELISA kits initially, and then were detected using one sandwich ELISA kit, and were confirmed by recombinent immunoblot assay (RIBA) and HCV-RNA.
  
  
  RESULTS
  To compared with HCV-RNA, false positive rate of two kinds of indirect ELISA and one sandwich ELISA was 40.9%, 59.1% and 2.2% respectively. The positive rates of them were 100%, but the analytical sensitivity of sandwich ELISA was more than indirect ELISA 2 to 6 times.
  
  
  CONCLUSIONS
  The sensitivity and specificity of sandwich ELISA was significantly better than those of indirect ELISA.
authors.affiliation: Lishui Blood Center, Lishui 323000, China.
authors.forename: Long-You
authors.initials: LY
authors.lastname: Zhao
authors.affiliation: Lishui Blood Center, Lishui 323000, China.
authors.forename: Yong-Ping
authors.initials: YP
authors.lastname: Ji
authors.affiliation: Lishui Blood Center, Lishui 323000, China.
authors.forename: De-Bin
authors.initials: DB
authors.lastname: Wang
authors.affiliation: Lishui Blood Center, Lishui 323000, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhuang
authors.affiliation: Lishui Blood Center, Lishui 323000, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006526:Hepatitis C / Q000097:blood / Q000175:diagnosis*; D018937:Hepatitis C Antibodies / Q000097:blood*; D006801:Humans; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D018937:Hepatitis C Antibodies; D011933:Reagent Kits, Diagnostic