title: ADRB3 gene promoter DNA methylation in blood and visceral adipose tissue is associated with metabolic disturbances in men.
abstract: OBJECTIVE
  ADRB3 DNA hypermethylation was recently associated with dyslipidemia in familial hypercholesterolemia (FH). In this study, we verified whether ADRB3 DNA methylation in blood and visceral adipose tissue (VAT) was associated with obesity and its related complications.
  
  
  METHODS
  DNA methylation levels were measured in the blood of 61 FH men, and the blood and VAT of 30 severely obese men. Common ADRB3 polymorphisms were genotyped in all subjects.
  
  
  RESULTS
  Higher ADRB3 DNA methylation levels were significantly associated with lower low-density lipoprotein cholesterol levels (r = -0.40; p = 0.01) in FH, and with a lower waist-to-hip ratio (r = -0.55; p = 0.01) and higher blood pressure (r = 0.43; p = 0.05) in severely obese men. ADRB3 g.-843C>T and p.W64R polymorphisms were found to be strongly associated (p < 0.001) with ADRB3 DNA methylation and mRNA levels.
  
  
  CONCLUSIONS
  Although further studies are needed, these results suggest that epigenetic changes at the ADRB3 gene locus might be involved in the development of obesity and its related metabolic complications.
authors.affiliation: Department of Biochemistry, Université de Sherbrooke, Sherbrooke, QC, Canada.
authors.forename: Simon-Pierre
authors.initials: SP
authors.lastname: Guay
authors.forename: Diane
authors.initials: D
authors.lastname: Brisson
authors.forename: Benoit
authors.initials: B
authors.lastname: Lamarche
authors.forename: Simon
authors.initials: S
authors.lastname: Biron
authors.forename: Odette
authors.initials: O
authors.lastname: Lescelleur
authors.forename: Laurent
authors.initials: L
authors.lastname: Biertho
authors.forename: Simon
authors.initials: S
authors.lastname: Marceau
authors.forename: Marie-Claude
authors.initials: MC
authors.lastname: Vohl
authors.forename: Daniel
authors.initials: D
authors.lastname: Gaudet
authors.forename: Luigi
authors.initials: L
authors.lastname: Bouchard
mesh_terms: D008078:Cholesterol, LDL / Q000378:metabolism; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D056726:Genetic Association Studies; D015894:Genome, Human; D006801:Humans; D006938:Hyperlipoproteinemia Type II / Q000235:genetics* / Q000473:pathology; D006973:Hypertension / Q000235:genetics*; D050152:Intra-Abdominal Fat / Q000378:metabolism*; D008297:Male; D009767:Obesity, Morbid / Q000150:complications / Q000235:genetics* / Q000473:pathology; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D040641:Quantitative Trait Loci; D012333:RNA, Messenger / Q000235:genetics; D022702:Receptors, Adrenergic, beta-3 / Q000097:blood* / Q000235:genetics*; D049629:Waist-Hip Ratio; D044465:White People
chemical_list: C568534:ADRB3 protein, human; D008078:Cholesterol, LDL; D012333:RNA, Messenger; D022702:Receptors, Adrenergic, beta-3