title: Evaluation of antioxidant and DNA damage protection activity of the hydroalcoholic extract of Desmostachya bipinnata L. Stapf.
abstract: Desmostachya bipinnata Stapf (Poaceae/Gramineae) is an official drug of ayurvedic pharmacopoeia. Various parts of this plant were used extensively in traditional and folklore medicine to cure various human ailments. The present study was aimed to evaluate the antioxidant and DNA damage protection activity of hydroalcoholic extract of Desmostachya bipinnata both in vitro and in vivo, to provide scientific basis for traditional usage of this plant. The extract showed significant antioxidant activity in a dose-dependent manner with an IC50 value of 264.18±3.47  μg/mL in H2O2 scavenging assay and prevented the oxidative damage to DNA in presence of DNA damaging agent (Fenton's reagent) at a concentration of 50  μg/mL. Also, the presence of extract protected yeast cells in a dose-dependent manner against DNA damaging agent (Hydroxyurea) in spot assay. Moreover, the presence of extract exhibited significant antioxidant activity in vivo by protecting yeast cells against oxidative stressing agent (H2O2). Altogether, the results of current study revealed that Desmostachya bipinnata is a potential source of antioxidants and lends pharmacological credence to the ethnomedical use of this plant in traditional system of medicine, justifying its therapeutic application for free-radical-induced diseases.
authors.affiliation: Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Bhopal, Bhopal, Madhya Pradesh 462023, India ; Department of Pharmacology, Faculty of Pharmaceutical Sciences, Bharat Institute of Technology, Hyderabad, Andhra Pradesh 501510, India.
authors.forename: Upendarrao
authors.identifier: 0000-0003-0519-7087
authors.initials: U
authors.lastname: Golla
authors.affiliation: Department of Pharmacology, Faculty of Pharmaceutical Sciences, Bharat Institute of Technology, Hyderabad, Andhra Pradesh 501510, India.
authors.forename: Solomon Sunder Raj
authors.initials: SS
authors.lastname: Bhimathati
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects*; D016166:Free Radical Scavengers / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000494:pharmacology*; D006109:Poaceae / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts; D006861:Hydrogen Peroxide