title: Identification, occurrence, and validation of DRE and ABRE Cis-regulatory motifs in the promoter regions of genes of Arabidopsis thaliana.
abstract: Plants posses a complex co-regulatory network which helps them to elicit a response under diverse adverse conditions. We used an in silico approach to identify the genes with both DRE and ABRE motifs in their promoter regions in Arabidopsis thaliana. Our results showed that Arabidopsis contains a set of 2,052 genes with ABRE and DRE motifs in their promoter regions. Approximately 72% or more of the total predicted 2,052 genes had a gap distance of less than 400 bp between DRE and ABRE motifs. For positional orientation of the DRE and ABRE motifs, we found that the DR form (one in direct and the other one in reverse orientation) was more prevalent than other forms. These predicted 2,052 genes include 155 transcription factors. Using microarray data from The Arabidopsis Information Resource (TAIR) database, we present 44 transcription factors out of 155 which are upregulated by more than twofold in response to osmotic stress and ABA treatment. Fifty-one transcripts from the one predicted above were validated using semiquantitative expression analysis to support the microarray data in TAIR. Taken together, we report a set of genes containing both DRE and ABRE motifs in their promoter regions in A. thaliana, which can be useful to understand the role of ABA under osmotic stress condition.
authors.affiliation: Biotechnology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, A Laboratory Under Council of Scientific and Industrial Research, Lucknow, 226015, India.
authors.forename: Sonal
authors.initials: S
authors.lastname: Mishra
authors.forename: Aparna
authors.initials: A
authors.lastname: Shukla
authors.forename: Swati
authors.initials: S
authors.lastname: Upadhyay
authors.lastname: Sanchita
authors.forename: Pooja
authors.initials: P
authors.lastname: Sharma
authors.forename: Seema
authors.initials: S
authors.lastname: Singh
authors.forename: Ujjal J
authors.initials: UJ
authors.lastname: Phukan
authors.forename: Abha
authors.initials: A
authors.lastname: Meena
authors.forename: Feroz
authors.initials: F
authors.lastname: Khan
authors.forename: Vineeta
authors.initials: V
authors.lastname: Tripathi
authors.forename: Rakesh Kumar
authors.initials: RK
authors.lastname: Shukla
authors.forename: Ashok
authors.initials: A
authors.lastname: Shrama
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins
keywords: Abscisic acid; abscisic acid-responsive elements; dehydration-responsive element; osmotic stress response