title: Titanium dioxide nanotube films: Preparation, characterization and electrochemical biosensitivity towards alkaline phosphatase.
abstract: Titania nanotubes (TNTs) were prepared by anodization on different substrates (titanium, Ti6Al4V and Ti6Al7Nb alloys) in ethylene glycol and glycerol. The influence of the applied potential and processing time on the nanotube diameter and length is analyzed. The as-formed nanotube layers are amorphous but they become crystalline when subjected to subsequent thermal treatment in air at 550°C; TNT layers grown on titanium and Ti6Al4V alloy substrates consist of anatase and rutile, while those grown on Ti6Al7Nb alloy consist only of anatase. The nanotube layers grown on Ti6Al7Nb alloy are less homogeneous, with supplementary islands of smaller diameter nanotubes, spread across the surface. Better adhesion and proliferation of osteoblasts was found for the nanotubes grown on all three substrates by comparison to an unprocessed titanium plate. The sensitivity towards bovine alkaline phosphatase was investigated mainly by electrochemical impedance spectroscopy in relation to the crystallinity, the diameter and the nature of the anodization electrolyte of the TNT/Ti samples. The measuring capacity of the annealed nanotubes of 50nm diameter grown in glycerol was demonstrated and the corresponding calibration curve was built for the concentration range of 0.005-0.1mg/mL.
authors.affiliation: S.C. METAV-Research and Development S.R.L., Bucharest, 31C. A. Rosetti, 020011, Romania. Electronic address: roman@metav-cd.ro.
authors.forename: Ioan
authors.initials: I
authors.lastname: Roman
authors.affiliation: S.C. METAV-Research and Development S.R.L., Bucharest, 31C. A. Rosetti, 020011, Romania.
authors.forename: Roxana Doina
authors.initials: RD
authors.lastname: Trusca
authors.affiliation: S.C. METAV-Research and Development S.R.L., Bucharest, 31C. A. Rosetti, 020011, Romania.
authors.forename: Maria-Laura
authors.initials: ML
authors.lastname: Soare
authors.affiliation: Research and Development National Institute for Nonferrous and Rare Metals, Pantelimon, 102 Biruintei, 077145, Romania.
authors.forename: Corneliu
authors.initials: C
authors.lastname: Fratila
authors.affiliation: University of Zielona Gora, Department of Biomedical Engineering Division, 9 Licealna, 65-417, Poland.
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Krasicka-Cydzik
authors.affiliation: University of Bucharest, Department of Biochemistry and Molecular Biology, 36-46 Mihail Kogalniceanu, 050107, Romania.
authors.forename: Miruna-Silvia
authors.initials: MS
authors.lastname: Stan
authors.affiliation: University of Bucharest, Department of Biochemistry and Molecular Biology, 36-46 Mihail Kogalniceanu, 050107, Romania.
authors.forename: Anca
authors.initials: A
authors.lastname: Dinischiotu
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis* / Q000378:metabolism / Q000592:standards; D000497:Alloys; D000818:Animals; D015374:Biosensing Techniques; D002138:Calibration; D002417:Cattle; D002460:Cell Line; D049109:Cell Proliferation; D003460:Crystallization; D058266:Dielectric Spectroscopy / Q000592:standards; D006801:Humans; D043942:Nanotubes / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D010316:Particle Size; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D000497:Alloys; C070282:Ti-6Al-7Nb alloy; C031462:titanium alloy (TiAl6V4); C009495:titanium dioxide; D014025:Titanium; D000469:Alkaline Phosphatase
keywords: Alkaline phosphatase; Anodization; Electrochemical impedance spectroscopy; Osteoblast; TiO(2) nanotube