title: Identification of the sulfone functionality in protonated analytes via ion/molecule reactions in a linear quadrupole ion trap mass spectrometer.
abstract: A tandem mass spectrometric method is presented for the rapid identification of drug metabolites that contain the sulfone functional group. This method is based on a gas-phase ion/molecule reaction of protonated sulfone analytes with trimethyl borate (TMB) that yields a diagnostic product ion, adduct-Me2O, at high reaction efficiency. A variety of compounds with different functional groups, such as sulfoxides, hydroxylamines, N-oxides, anilines, phenol, an aliphatic amine, and an aliphatic alcohol, were examined to probe the selectivity of this reaction. Except for protonated sulfones, most of the protonated compounds react very slowly or not at all with TMB. Most importantly, none of them give the adduct-Me2O product. A mechanism that explains the observed selectivity is proposed for the diagnostic reaction and is supported by quantum chemical calculations. The reaction was tested with the anti-inflammatory drug sulindac and its metabolite, sulindac sulfone, which were readily distinguished. The presence of other functionalities in addition to sulfone was found not to influence the diagnostic reactivity.
authors.affiliation: Department of Chemistry, Purdue University , West Lafayette, Indiana 47907, United States.
authors.forename: Huaming
authors.initials: H
authors.lastname: Sheng
authors.forename: Peggy E
authors.initials: PE
authors.lastname: Williams
authors.forename: Weijuan
authors.initials: W
authors.lastname: Tang
authors.forename: James S
authors.initials: JS
authors.lastname: Riedeman
authors.forename: Minli
authors.initials: M
authors.lastname: Zhang
authors.forename: Hilkka I
authors.initials: HI
authors.lastname: Kenttämaa
mesh_terms: D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000494:pharmacology*; D001881:Borates / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D007477:Ions / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D011789:Quantum Theory; D013450:Sulfones / Q000032:analysis* / Q000737:chemistry*; D013467:Sulindac / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D053719:Tandem Mass Spectrometry
chemical_list: D000893:Anti-Inflammatory Agents; D001881:Borates; D007477:Ions; D013450:Sulfones; D013467:Sulindac; C102081:trimethyl borate; C025463:sulindac sulfone