title: The glutathione peroxidase gene family in Thellungiella salsuginea: genome-wide identification, classification, and gene and protein expression analysis under stress conditions.
abstract: Glutathione peroxidases (GPX) catalyze the reduction of H2O2 or organic hydroperoxides to water or corresponding alcohols using reduced glutathione, which plays an essential role in ROS (reactive oxygen species) homeostasis and stress signaling. Thellungiella salsuginea (Eutrema salsugineum), a relative of Arabidopsis thaliana, displays an extremely high level of tolerance to salt, drought, cold and oxidative stresses. The enzymatic antioxidant systems may contribute to the stress tolerance of T. salsuginea. In the present study, we aimed at understanding the roles of the antioxidant enzymes in T. salsuginea by focusing on the GPX family. We identified the eight GPX genes in T. salsuginea, and the structure of the N-terminal domains indicated their putative chloroplastic, mitochondrial and cytoplasmic location. The exon-intron organization of these genes exhibited a conserved pattern among plant GPX genes. Multiple environmental stresses and hormone response related cis-acting elements were predicted in the promoters of TsGPX genes. The gene and protein expression profiles of TsGPXs in response to high level of salinity and osmotic stresses, in leaves and roots of T. salsuginea were investigated using real-time RT-PCR and western blotting analysis. Our result showed that different members of the GPX gene family were coordinately regulated under specific environmental stress conditions, and supported the important roles of TsGPXs in salt and drought stress response in T. salsuginea.
authors.affiliation: College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China. gaofei@muc.edu.cn.
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.affiliation: College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China. wendybj121@126.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China. ggtg00@163.com.
authors.forename: Tingting
authors.initials: T
authors.lastname: Ma
authors.affiliation: College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China. lihuayun0331@163.com.
authors.forename: Huayun
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China. wzpn123@163.com.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.affiliation: College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China. lizhanglei2008@126.com.
authors.forename: Zhanglei
authors.initials: Z
authors.lastname: Li
authors.affiliation: College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China. zzcjianao1@163.com.
authors.forename: Zichen
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China. zhouyijun@muc.edu.cn.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics; D019607:Brassicaceae / Q000201:enzymology* / Q000235:genetics; D030541:Databases, Genetic; D018745:Genome, Plant; D005979:Glutathione Peroxidase / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D020033:Protein Isoforms / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment; D013312:Stress, Physiological; D059467:Transcriptome
chemical_list: D010940:Plant Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D005979:Glutathione Peroxidase