title: Long-range interaction and correlation between MYC enhancer and oncogenic long noncoding RNA CARLo-5.
abstract: The mechanism by which the 8q24 MYC enhancer region, including cancer-associated variant rs6983267, increases cancer risk is unknown due to the lack of protein-coding genes at 8q24.21. Here we report the identification of long noncoding RNAs named cancer-associated region long noncoding RNAs (CARLos) in the 8q24 region. The expression of one of the long noncoding RNAs, CARLo-5, is significantly correlated with the rs6983267 allele associated with increased cancer susceptibility. We also found the MYC enhancer region physically interacts with the active regulatory region of the CARLo-5 promoter, suggesting long-range interaction of MYC enhancer with the CARLo-5 promoter regulates CARLo-5 expression. Finally, we demonstrate that CARLo-5 has a function in cell-cycle regulation and tumor development. Overall, our data provide a key of the mystery of the 8q24 gene desert.
authors.affiliation: Department of Molecular Virology, Immunology and Medical Genetics and Department of Anesthesiology, Wexner Medical Center, and Department of Neurological Surgery, The Ohio State University, Columbus, OH 43210.
authors.forename: Taewan
authors.initials: T
authors.lastname: Kim
authors.forename: Ri
authors.initials: R
authors.lastname: Cui
authors.forename: Young-Jun
authors.initials: YJ
authors.lastname: Jeon
authors.forename: Ji-Hoon
authors.initials: JH
authors.lastname: Lee
authors.forename: Ju Hee
authors.initials: JH
authors.lastname: Lee
authors.forename: Hosung
authors.initials: H
authors.lastname: Sim
authors.forename: Jong Kook
authors.initials: JK
authors.lastname: Park
authors.forename: Paolo
authors.initials: P
authors.lastname: Fadda
authors.forename: Esmerina
authors.initials: E
authors.lastname: Tili
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakanishi
authors.forename: Man-Il
authors.initials: MI
authors.lastname: Huh
authors.forename: Sung-Hak
authors.initials: SH
authors.lastname: Kim
authors.forename: Ju Hwan
authors.initials: JH
authors.lastname: Cho
authors.forename: Bong Hwan
authors.initials: BH
authors.lastname: Sung
authors.forename: Yong
authors.initials: Y
authors.lastname: Peng
authors.forename: Tae Jin
authors.initials: TJ
authors.lastname: Lee
authors.forename: Zhenghua
authors.initials: Z
authors.lastname: Luo
authors.forename: Hui-Lung
authors.initials: HL
authors.lastname: Sun
authors.forename: Huijun
authors.initials: H
authors.lastname: Wei
authors.forename: Hansjuerg
authors.initials: H
authors.lastname: Alder
authors.forename: Jeong Su
authors.initials: JS
authors.lastname: Oh
authors.forename: Kang Sup
authors.initials: KS
authors.lastname: Shim
authors.forename: Sang-Bong
authors.initials: SB
authors.lastname: Ko
authors.forename: Carlo M
authors.initials: CM
authors.lastname: Croce
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D004742:Enhancer Elements, Genetic / Q000235:genetics; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D062085:RNA, Long Noncoding / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D062085:RNA, Long Noncoding; D034741:RNA, Small Interfering