title: Supplement timing of cranberry extract plays a key role in promoting Caenorhabditis elegans healthspan.
abstract: Consumption of nutraceuticals is a major and potent dietary intervention for delaying aging. As the timing of administration is critical for the efficacy of bioactive compounds in medicine, the effectiveness of nutraceuticals may also be dramatically affected by the timing of supplementation. Cranberry exact (CBE), rich in polyphenols, is consumed as a nutraceutical, and possesses anti-aging properties. Here, we examined the influence of timing on the beneficial effects of CBE supplementation in C. elegans. The prolongevity effect of CBE in different aged worms, young adults, middle-age adults, and aged adults, was determined. Early-start intervention with CBE prolonged the remaining lifespan of worms of different ages more robustly than late-start intervention. The effectiveness of CBE on stress responses and physiological behaviors in different aged worms was also investigated. The early-start intervention prominently promoted motility and resistance to heat shocks and V. cholera infection, especially in aged worms. Together, these findings suggest that the timing of CBE supplementation critically influences its beneficial effects on C. elegans lifespan and healthspan. It is of interest to further investigate whether the similar results would occur in humans.
authors.affiliation: Department of Biological Sciences, Clemson University, Clemson, SC 29634, USA. sguha@g.clemson.edu.
authors.forename: Sujay
authors.initials: S
authors.lastname: Guha
authors.affiliation: Department of Biological Sciences, Clemson University, Clemson, SC 29634, USA. onatara@clemson.edu.
authors.forename: Ojas
authors.initials: O
authors.lastname: Natarajan
authors.affiliation: Department of Biological Sciences, Clemson University, Clemson, SC 29634, USA. cmurbac@g.clemson.edu.
authors.forename: Cole G
authors.initials: CG
authors.lastname: Murbach
authors.affiliation: Department of Biological Sciences, Clemson University, Clemson, SC 29634, USA. jdinh@g.clemson.edu.
authors.forename: Jessica
authors.initials: J
authors.lastname: Dinh
authors.affiliation: Department of Biological Sciences, Clemson University, Clemson, SC 29634, USA. pelamisplaturus@gmail.com.
authors.forename: Ethan C
authors.initials: EC
authors.lastname: Wilson
authors.affiliation: Department of Biological Sciences, Clemson University, Clemson, SC 29634, USA. mcao@clemson.edu.
authors.forename: Min
authors.initials: M
authors.lastname: Cao
authors.affiliation: Functional Genomics Unit, Translational Gerontology Branch, National Institute on Aging, Baltimore, MD 21224, USA. zous@grc.nia.nih.gov.
authors.forename: Sige
authors.initials: S
authors.lastname: Zou
authors.affiliation: Department of Biological Sciences, Clemson University, Clemson, SC 29634, USA. ydong@clemson.edu.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Dong
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000502:physiology*; D019587:Dietary Supplements; D005638:Fruit / Q000737:chemistry; D008136:Longevity / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology; D029799:Vaccinium macrocarpon / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D059808:Polyphenols