title: JAK2-centered interactome hotspot identified by an integrative network algorithm in acute Stanford type A aortic dissection.
abstract: The precise mechanisms underlying dissections, especially those without connective tissue diseases or congenital vascular diseases, are incompletely understood. This study attempted to identify both the expression profile of the dissected ascending aorta and the interactome hotspots associated with the disease, using microarray technology and gene regulatory network analysis. There were 2,737 genes differentially expressed between patients with acute Stanford type A aortic dissection and controls. Eight interactome hotspots significantly associated with aortic dissection were identified by an integrative network algorithm. In particular, we identified a JAK2-centered expression module, which was validated in an independent gene expression microarray data set, and which was characterized by over-expressed cytokines and receptors in acute aortic dissection cases, indicating that JAK2 may play a key role in the inflammatory process, which potentially contributes to the occurrence of acute aortic dissection. Overall, the analytical strategy used in this study offered the possibility to identify functional relevant network modules and subsequently facilitated the biological interpretation in the complicated disease.
authors.affiliation: Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
authors.forename: Sun
authors.initials: S
authors.lastname: Pan
authors.affiliation: Biomedical Research Center, Zhongshan Hospital, Fudan University, Shanghai, China.
authors.forename: Duojiao
authors.initials: D
authors.lastname: Wu
authors.affiliation: UCL Cancer Institute, University College London, London, United Kingdom ; CAS-MPG Partner Institute for Computational Biology, Shanghai, China.
authors.forename: Andrew E
authors.initials: AE
authors.lastname: Teschendorff
authors.affiliation: Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Hong
authors.affiliation: Biomedical Research Center, Zhongshan Hospital, Fudan University, Shanghai, China.
authors.forename: Linyan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Biomedical Research Center, Zhongshan Hospital, Fudan University, Shanghai, China.
authors.forename: Mengjia
authors.initials: M
authors.lastname: Qian
authors.affiliation: Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Wang
authors.affiliation: Biomedical Research Center, Zhongshan Hospital, Fudan University, Shanghai, China.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Wang
mesh_terms: D000328:Adult; D000465:Algorithms; D000784:Aortic Dissection / Q000235:genetics*; D001011:Aorta / Q000378:metabolism; D017545:Aortic Aneurysm, Thoracic / Q000235:genetics*; D005260:Female; D053263:Gene Regulatory Networks / Q000235:genetics*; D006801:Humans; D053614:Janus Kinase 2 / Q000235:genetics*; D008297:Male; D059467:Transcriptome / Q000235:genetics
chemical_list: C507924:JAK2 protein, human; D053614:Janus Kinase 2