title: Dramatic reduction of culture time of Mycobacterium tuberculosis.
abstract: Mycobacterium tuberculosis culture, a critical technique for routine diagnosis of tuberculosis, takes more than two weeks. Here, step-by-step improvements in the protocol including a new medium, microaerophlic atmosphere or ascorbic-acid supplement and autofluorescence detection dramatically shortened this delay. In the best case, primary culture and rifampicin susceptibility testing were achieved in 72 hours when specimens were inoculated directly on the medium supplemented by antibiotic at the beginning of the culture.
authors.affiliation: Aix Marseille Université, URMITE UMR CNRS 7278, IRD 198, Inserm 1095, 13005, Marseille, France.
authors.forename: Ramzi
authors.initials: R
authors.lastname: Ghodbane
authors.affiliation: Aix Marseille Université, URMITE UMR CNRS 7278, IRD 198, Inserm 1095, 13005, Marseille, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Raoult
authors.affiliation: Aix Marseille Université, URMITE UMR CNRS 7278, IRD 198, Inserm 1095, 13005, Marseille, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Drancourt
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001205:Ascorbic Acid / Q000378:metabolism*; D061249:Batch Cell Culture Techniques / Q000379:methods*; D001681:Biological Assay / Q000379:methods*; D019149:Bioreactors / Q000382:microbiology*; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D003470:Culture Media / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000502:physiology*; D012293:Rifampin / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D001205:Ascorbic Acid; D012293:Rifampin