title: Electrochemical control of the enzymatic polymerization of PEG hydrogels: formation of spatially controlled biological microenvironments.
abstract: Control of pH gradient profile at the electrode-electrolyte interfaces allows the control of the enzymatic PEG-hydrogel polymerization. By tuning the solution pH, buffer capacity, and the applied current, the extent of the local inhibition and confinement of the Factor XIII-mediated polymerization of PEG are controlled. This technology opens new perspectives for the production of 3D-structured biological microenvironments.
authors.affiliation: Laboratory for Cell and Tissue Engineering, Department of Obstetrics, University Hospital Zurich, Schmelzbergstrasse 12, PATH G 48b, 8091, Zurich, Switzerland.
authors.forename: Vincent
authors.initials: V
authors.lastname: Milleret
authors.forename: Benjamin R
authors.initials: BR
authors.lastname: Simona
authors.forename: Philipp S
authors.initials: PS
authors.lastname: Lienemann
authors.forename: János
authors.initials: J
authors.lastname: Vörös
authors.forename: Martin
authors.initials: M
authors.lastname: Ehrbar
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D060846:Cell Engineering / Q000379:methods*; D002460:Cell Line; D060833:Cellular Microenvironment; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000378:metabolism; D058105:Polymerization
chemical_list: D001672:Biocompatible Materials; D004129:Dimethylpolysiloxanes; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols; C013830:baysilon
keywords: PEG-hydrogels; electrochemistry; microenvironment