title: Comparison of 2 proposed MLVA protocols for subtyping non-O157:H7 verotoxigenic Escherichia coli.
abstract: Multiple locus variable number tandem repeats (VNTRs) analysis (MLVA) has become a reliable tool, able to establish genetic relationships for epidemiological surveillance and molecular subtyping of pathogens such as verotoxigenic Escherichia coli (VTEC). This emerging pathogen whose primary reservoir is the cattle causes severe disease in humans, such as hemorrhagic colitis and hemolytic uremic syndrome. With the aim of comparing a recently proposed MLVA assay with that used routinely in our laboratory, we analyzed a set of VTEC isolates (n = 72) obtained from meat using both assays. All samples could be typed by the new MLVA assay, and an increase in the number of distinct profiles (31-43) was observed. However, intraserotype resolution was not significantly enhanced; thus, the incorporation of more VNTR loci is still needed to achieve a greater discrimination among non-O157:H7 serotypes.
authors.affiliation: Laboratorio de Inmunoquímica y Biotecnología, CIVETAN-Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), 7000 Tandil, Argentina; Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CIC-PBA), Argentina.
authors.forename: Juliana
authors.initials: J
authors.lastname: González
authors.affiliation: Laboratorio de Inmunoquímica y Biotecnología, CIVETAN-Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), 7000 Tandil, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: Andrea Mariel
authors.initials: AM
authors.lastname: Sanso
authors.affiliation: Laboratorio de Inmunoquímica y Biotecnología, CIVETAN-Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), 7000 Tandil, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: Paula María Alejandra
authors.initials: PM
authors.lastname: Lucchesi
authors.affiliation: Laboratorio de Inmunoquímica y Biotecnología, CIVETAN-Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), 7000 Tandil, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. Electronic address: avbustaman@vet.unicen.edu.ar.
authors.forename: Ana Victoria
authors.initials: AV
authors.lastname: Bustamante
mesh_terms: D000818:Animals; D002417:Cattle; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D008460:Meat / Q000382:microbiology; D018598:Minisatellite Repeats; D017720:Molecular Epidemiology / Q000379:methods; D058889:Molecular Typing / Q000379:methods*; D054323:Shiga-Toxigenic Escherichia coli / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial
keywords: MLVA; Non-O157 serotypes; Raw products; VTEC