title: Odontogenic differentiation and dentin formation of dental pulp cells under nanobioactive glass induction.
abstract: Bioactive glass (BG) has been widely used in bone regeneration; however, reports on the biological effects of BG on dental pulp cells are rare. This study aims to investigate the effects of nanoscale BG (n-BG) on odontogenic differentiation and dentin formation of dental pulp cells and to compare these effects with those of microscale BG (m-BG). Human dental pulp cells (hDPCs) from third molars were cultured directly with m-BG and n-BG in vitro. The cell proliferation increased at 0.1mgml(-1) BG, which also had a chemotactic effect on hDPCs. The mineralization capacity and expression of odontogenic-related proteins and genes (dentin sialophosphoprotein, dentin matrix protein 1 and collagen type I) of hDPCs were significantly up-regulated under BG induction, and were particularly higher in the n-BG group than in the control group. m-BG and n-BG combined with pulp tissues were transplanted into the dorsum of immunodeficient mice to observe their biological effects on dental pulp cells in vivo. A continuous layer of dentin-like tissue with uniform thickness, a well-organized dentinal tubule structure and polarizing odontoblast-like cells aligned along it was generated upon the n-BG layer, whereas some irregular sporadic osteodentin-like mineralized tissues were observed in the control group. This study reveals that BG, especially n-BG, induces the odontogenic differentiation and dentin formation of dental pulp cells and may serve as a potential material for pulp repair and dentin regeneration.
authors.affiliation: Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology, 22 Zhongguancun Nandajie, Haidian District, Beijing 100081, People's Republic of China.
authors.forename: Sainan
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology, 22 Zhongguancun Nandajie, Haidian District, Beijing 100081, People's Republic of China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Gao
authors.affiliation: Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology, 22 Zhongguancun Nandajie, Haidian District, Beijing 100081, People's Republic of China.
authors.forename: Weiyu
authors.initials: W
authors.lastname: Gong
authors.affiliation: Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology, 22 Zhongguancun Nandajie, Haidian District, Beijing 100081, People's Republic of China.
authors.forename: Zhichun
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510640, People's Republic of China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology, 22 Zhongguancun Nandajie, Haidian District, Beijing 100081, People's Republic of China. Electronic address: kqdongyanmei@bjmu.edu.cn.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Dong
mesh_terms: D017209:Apoptosis; D001483:Base Sequence; D001672:Biocompatible Materials; D002454:Cell Differentiation; D017931:DNA Primers; D003782:Dental Pulp / Q000166:cytology / Q000378:metabolism*; D003804:Dentin / Q000378:metabolism*; D005898:Glass; D006801:Humans; D020287:In Situ Nick-End Labeling; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014070:Tooth / Q000166:cytology*
chemical_list: D001672:Biocompatible Materials; D017931:DNA Primers
keywords: Bioactive glass; Dental pulp cells; Dentin regeneration; Nanoparticles; Odontogenic differentiation