title: Effect of NK4 transduction in bone marrow-derived mesenchymal stem cells on biological characteristics of pancreatic cancer cells.
abstract: Pancreatic cancer usually has a poor prognosis, and no gene therapy has yet been developed that is effective to treat it. Since a unique characteristic of bone marrow-derived mesenchymal stem cells (MSCs) is that they migrate to tumor tissues, we wanted to determine whether MSCs could serve as a vehicle of gene therapy for targeting pancreatic cancer. First, we successfully extracted MSCs from SD rats. Next, MSCs were efficiently transduced with NK4, an antagonist of hepatocyte growth factor (HGF) which comprising the N-terminal and the subsequent four kringle domains of HGF, by an adenoviral vector. Then, we confirmed that rat MSCs preferentially migrate to pancreatic cancer cells. Last, MSCs expressing NK4 (NK4-MSCs) strongly inhibited proliferation and migration of the pancreatic cancer cell line SW1990 after co-culture. These results indicate that MSCs can serve as a vehicle of gene therapy for targeting pancreatic cancer.
authors.affiliation: Department of Hepatobiliary Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, China. syfsjb@sina.com.
authors.forename: Yun-Peng
authors.initials: YP
authors.lastname: Sun
authors.affiliation: Department of General Surgery, Yiwu Chouzhou Hospital, Yiwu 322000, Zhejiang, China. zhangbenlong0021@126.com.
authors.forename: Ben-Long
authors.initials: BL
authors.lastname: Zhang
authors.affiliation: Department of Hepatobiliary Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, China. djw3522@163.com.
authors.forename: Jian-Wen
authors.initials: JW
authors.lastname: Duan
authors.affiliation: Department of Infectious Disease, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, China. wuhh19@sina.com.
authors.forename: Huan-Huan
authors.initials: HH
authors.lastname: Wu
authors.affiliation: Department of Hepatobiliary Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, China. wangbeq@sohu.com.
authors.forename: Ben-Quan
authors.initials: BQ
authors.lastname: Wang
authors.affiliation: Department of Hepatobiliary Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, China. yjping45@tom.com.
authors.forename: Zheng-Ping
authors.initials: ZP
authors.lastname: Yu
authors.affiliation: Department of Hepatobiliary Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, China. greatywj@sohu.com.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Yang
authors.affiliation: Department of Hepatobiliary Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, China. syfsjb@sina.com.
authors.forename: Yun-Feng
authors.initials: YF
authors.lastname: Shan
authors.affiliation: Department of Hepatobiliary Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, China. zmtzmtao@126.com.
authors.forename: Meng-Tao
authors.initials: MT
authors.lastname: Zhou
authors.affiliation: Department of Hepatobiliary Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, China. zhangqiyu0123@126.com.
authors.forename: Qi-Yu
authors.initials: QY
authors.lastname: Zhang
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D057809:HEK293 Cells; D017228:Hepatocyte Growth Factor / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D010190:Pancreatic Neoplasms / Q000473:pathology; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014161:Transduction, Genetic
chemical_list: C066855:HGF protein, human; D017228:Hepatocyte Growth Factor