title: Natural polyhydroxyalkanoate-gold nanocomposite based biosensor for detection of antimalarial drug artemisinin.
abstract: The worrisome trend of antimalarial resistance has already highlighted the importance of artemisinin as a potent antimalarial agent. The current investigation aimed at fabricating a biosensor based on natural polymer polyhydroxyalkanoate-gold nanoparticle composite mounting on an indium-tin oxide glass plate for the analysis of artemisinin. The biosensor was fabricated using an adsorbing horse-radish peroxidase enzyme on the electrode surface for which cyclic voltammetry was used to monitor the electro-catalytic reduction of artemisinin under diffusion controlled conditions. Electrochemical interfacial properties and immobilization of enzyme onto a polyhydroxyalkanoate-gold nanoparticle film were evaluated, and confirmed by cyclic voltammetry, electrochemical impedance spectroscopy and scanning electron microscopy. The differential pulse voltammetric peak current for artemisinin was increased linearly (concentration range of 0.01-0.08μg mL(-1)) with sensitivity of 0.26μAμg mL(-1). The greater sensitivity of the fabricated biosensor to artemisinin (optimum limits of detection were 0.0035μg mL(-1) and 0.0036μg mL(-1) in bulk and spiked human serum, respectively) could be of much aid in medical diagnosis.
authors.affiliation: Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India.
authors.forename: Pinkee
authors.initials: P
authors.lastname: Phukon
authors.affiliation: Analytical Chemistry Division, CSIR-North East Institute of Science & Technology, Jorhat 785006, Assam, India.
authors.forename: Keisham
authors.initials: K
authors.lastname: Radhapyari
authors.affiliation: Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India; Nagaland University (Central), Lumami, Zunheboto, Nagaland 798627, India.
authors.forename: Bolin Kumar
authors.initials: BK
authors.lastname: Konwar
authors.affiliation: Analytical Chemistry Division, CSIR-North East Institute of Science & Technology, Jorhat 785006, Assam, India. Electronic address: khan.raju@gmail.com.
authors.forename: Raju
authors.initials: R
authors.lastname: Khan
mesh_terms: D000962:Antimalarials / Q000032:analysis* / Q000097:blood; D037621:Artemisinins / Q000032:analysis* / Q000097:blood; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005898:Glass / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D054813:Polyhydroxyalkanoates / Q000737:chemistry*; D017971:Tin Compounds / Q000737:chemistry
chemical_list: D000962:Antimalarials; D037621:Artemisinins; D004800:Enzymes, Immobilized; D054813:Polyhydroxyalkanoates; D017971:Tin Compounds; C109984:indium tin oxide; D006046:Gold; C031327:artemisinin; D006735:Horseradish Peroxidase
keywords: Artemisinin; Biopolymer; Biosensors; Electrochemical impedance spectroscopy; Horse-radish peroxidase