title: Hydrogen sulfide accelerates cell cycle progression in oral squamous cell carcinoma cell lines.
abstract: OBJECTIVE
  To investigate the cell cycle regulator role of the third gaseous transmitter hydrogen sulfide (H2 S) in three oral SCC cell lines by using NaHS, a donor of H2 S.
  
  
  METHODS
  The synchronized oral squamous cell carcinoma cell lines (Cal27, GNM, and WSU-HN6) were treated with different concentrations of NaHS and then subjected to cell proliferation, cell cycle, and Western blot analyses.
  
  
  RESULTS
  The CCK-8 assay results showed that the exogenously administered H2 S donor, NaHS, induced CAL-27, and GNM cell proliferation in a concentration-dependent manner, and the cell cycle analysis indicated that NaHS accelerated cell cycle progression of the synchronized CAL-27, GNM, and WSU-HN6 cells. Western blot analysis revealed that the cell cycle regulatory genes RPA70 and RB1 were significantly down-regulated and that proliferating cell nuclear antigen (PCNA) and CDK4 were markedly up-regulated by NaHS at specific time points in the cell cycle. In addition, our results indicated that the phosphorylation of Akt and Erk1/2 was involved in exogenous H2 S-induced oral SCC cell proliferation.
  
  
  CONCLUSIONS
  H2 S is a potential pro-proliferative factor of human oral SCC cells that accelerates the progression of the SCC cell cycle; thus, H2 S plays a deleterious role in oral SCC cancer development.
authors.affiliation: Department of Special Dentistry, Peking University School and Hospital of Stomatology, Beijing, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Ma
authors.forename: Q
authors.initials: Q
authors.lastname: Bi
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D051358:Cyclin-Dependent Kinase 4 / Q000187:drug effects / Q000235:genetics; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006258:Head and Neck Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D009062:Mouth Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D010766:Phosphorylation; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D051716:Replication Protein A / Q000187:drug effects / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects; D000077195:Squamous Cell Carcinoma of Head and Neck; D013440:Sulfides / Q000494:pharmacology; D015854:Up-Regulation
chemical_list: D018809:Proliferating Cell Nuclear Antigen; C497167:RPA1 protein, human; D051716:Replication Protein A; D013440:Sulfides; C495900:CDK4 protein, human; D051358:Cyclin-Dependent Kinase 4; C025451:sodium bisulfide; D006862:Hydrogen Sulfide
keywords: cell cycle; hydrogen sulfide; proliferation; squamous cell carcinoma