title: Cloud point extraction-HPLC method for the determination and pharmacokinetic study of aristolochic acids in rat plasma after oral administration of Aristolochiae Fructus.
abstract: Based on cloud-point extraction (CPE), a high performance liquid chromatography method (HPLC) was developed and validated for the determination of aristolochic acids (AAs) in rat plasma after oral administration of Aristolochiae Fructus (AF). Non-ionic surfactant Genapol X-080, an environmentally friendly solvent, was used for the micelle-mediated extraction. Various influencing factors on CPE process were investigated and optimized. AAs were extracted from rat plasma after adding 1ml of 4.5% (v/v) surfactant in the presence of 0.2mol/l HCl and 20mg NaCl, and the incubation temperature and time were 50°C and 10min, respectively. Base-line separation was obtained for the AAs in rat plasma with the optimized chromatography conditions. The detection limits (LOD) reached downward 10ng/ml. The intra-day and inter-day precisions were less than 7.8%, the accuracies were within ±5.5%, and the average recovery factors were in the range of 94.5-105.4%. In comparison with liquid-liquid extraction, the CPE method has a considerable LOD and higher recoveries. The proposed CPE-HPLC method was specific, sensitive and reliable, and could be an effective tool for the determination of AAs in biological matrixes. With the method the pharmacokinetics of AAs were investigated successfully after oral administration of AF by rats.
authors.affiliation: Key Laboratory of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Ren
authors.affiliation: Key Laboratory of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Chemo/Biosensing & Chemometrics, Chemistry & Chemical Engineering College, Hunan University, Changsha 410082, China.
authors.forename: Jiangang
authors.initials: J
authors.lastname: Wu
authors.affiliation: Key Laboratory of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China. Electronic address: kings2008@163.com.
authors.forename: Jinbin
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
authors.forename: Gaihong
authors.initials: G
authors.lastname: Yang
authors.affiliation: Key Laboratory of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China; State Key Laboratory of Chemo/Biosensing & Chemometrics, Chemistry & Chemical Engineering College, Hunan University, Changsha 410082, China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Chemo/Biosensing & Chemometrics, Chemistry & Chemical Engineering College, Hunan University, Changsha 410082, China. Electronic address: szyao@hnu.cn.
authors.forename: Shouzhuo
authors.initials: S
authors.lastname: Yao
mesh_terms: D000284:Administration, Oral; D000818:Animals; D029065:Aristolochiaceae / Q000737:chemistry*; D034341:Aristolochic Acids / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005638:Fruit / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D034341:Aristolochic Acids; D004365:Drugs, Chinese Herbal
keywords: Aristolochiae Fructus; Aristolochic acids; Cloud point extraction; Genapol X-080; HPLC; Rat plasma