title: Chloride intracellular channel 1 regulates colon cancer cell migration and invasion through ROS/ERK pathway.
abstract: OBJECTIVE
  To investigate the mechanisms of chloride intracellular channel 1 (CLIC1) in the metastasis of colon cancer under hypoxia-reoxygenation (H-R) conditions.
  
  
  METHODS
  Fluorescent probes were used to detect reactive oxygen species (ROS) in LOVO cells. Wound healing assay and transwell assay were performed to examine the migration and invasion of LOVO cells. Expression of CLIC1 mRNA and protein, p-ERK, MMP-2 and MMP-9 proteins was analyzed by reverse transcription-polymerase chain reaction and Western blot.
  
  
  METHODS
  H-R treatment increased the intracellular ROS level in LOVO cells. The mRNA and protein expression of CLIC1 was elevated under H-R conditions. Functional inhibition of CLIC1 markedly decreased the H-R-enhanced ROS generation, cell migration, invasion and phosphorylation of ERK in treated LOVO cells. Additionally, the expression of MMP-2 and MMP-9 could be regulated by CLIC1-mediated ROS/ERK pathway.
  
  
  CONCLUSIONS
  Our results suggest that CLIC1 protein is involved in the metastasis of colon cancer LOVO cells via regulating the ROS/ERK pathway in the H-R process.
authors.affiliation: Pan Wang, Department of General Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong 637007, Sichuan Province, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Wang
authors.affiliation: Pan Wang, Department of General Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong 637007, Sichuan Province, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Pan Wang, Department of General Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong 637007, Sichuan Province, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.affiliation: Pan Wang, Department of General Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong 637007, Sichuan Province, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Pan Wang, Department of General Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong 637007, Sichuan Province, China.
authors.forename: Pei-Wu
authors.initials: PW
authors.lastname: Yu
authors.affiliation: Pan Wang, Department of General Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong 637007, Sichuan Province, China.
authors.forename: Ying-Xue
authors.initials: YX
authors.lastname: Hao
authors.affiliation: Pan Wang, Department of General Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong 637007, Sichuan Province, China.
authors.forename: Hua-Xin
authors.initials: HX
authors.lastname: Luo
authors.affiliation: Pan Wang, Department of General Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong 637007, Sichuan Province, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D018118:Chloride Channels / Q000378:metabolism*; D003094:Collagen / Q000737:chemistry; D003110:Colonic Neoplasms / Q000378:metabolism*; D004338:Drug Combinations; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D005456:Fluorescent Dyes; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D000860:Hypoxia; D007797:Laminin / Q000737:chemistry; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D011509:Proteoglycans / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction; D014945:Wound Healing
chemical_list: C501966:CLIC1 protein, human; D018118:Chloride Channels; D004338:Drug Combinations; D005456:Fluorescent Dyes; D007797:Laminin; D011509:Proteoglycans; D017382:Reactive Oxygen Species; C059485:matrigel; D003094:Collagen; D048049:Extracellular Signal-Regulated MAP Kinases; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: Cancer invasion; Colon cancer; Extracellular signal-regulated kinase; Hypoxia-reoxygenation; Intracellular chloride channel 1; Reactive oxygen species