title: A regulatory network of Drosophila germline stem cell self-renewal.
abstract: Stem cells possess the capacity to generate two cells of distinct fate upon division: one cell retaining stem cell identity and the other cell destined to differentiate. These cell fates are established by cell-type-specific genetic networks. To comprehensively identify components of these networks, we performed a large-scale RNAi screen in Drosophila female germline stem cells (GSCs) covering ∼25% of the genome. The screen identified 366 genes that affect GSC maintenance, differentiation, or other processes involved in oogenesis. Comparison of GSC regulators with neural stem cell self-renewal factors identifies common and cell-type-specific self-renewal genes. Importantly, we identify the histone methyltransferase Set1 as a GSC-specific self-renewal factor. Loss of Set1 in neural stem cells does not affect cell fate decisions, suggesting a differential requirement of H3K4me3 in different stem cell lineages. Altogether, our study provides a resource that will help to further dissect the networks underlying stem cell self-renewal.
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Dong
authors.initials: D
authors.lastname: Yan
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Ralph A
authors.initials: RA
authors.lastname: Neumüller
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, MA 02115, USA; Drosophila RNAi Screening Center, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Buckner
authors.affiliation: Departments of Genetics and Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA.
authors.forename: Kathleen
authors.initials: K
authors.lastname: Ayers
authors.affiliation: Stowers Institute for Medical Research, Kansas City, MO 64110, USA.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, MA 02115, USA; Drosophila RNAi Screening Center, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, MA 02115, USA; Drosophila RNAi Screening Center, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Donghui
authors.initials: D
authors.lastname: Yang-Zhou
authors.affiliation: Stowers Institute for Medical Research, Kansas City, MO 64110, USA.
authors.forename: Lei
authors.initials: L
authors.lastname: Pan
authors.affiliation: Stowers Institute for Medical Research, Kansas City, MO 64110, USA.
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Colleen
authors.initials: C
authors.lastname: Kelley
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Arunachalam
authors.initials: A
authors.lastname: Vinayagam
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Binari
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Sakara
authors.initials: S
authors.lastname: Randklev
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, MA 02115, USA; Drosophila RNAi Screening Center, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Lizabeth A
authors.initials: LA
authors.lastname: Perkins
authors.affiliation: Stowers Institute for Medical Research, Kansas City, MO 64110, USA.
authors.forename: Ting
authors.initials: T
authors.lastname: Xie
authors.affiliation: Departments of Genetics and Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA.
authors.forename: Lynn
authors.initials: L
authors.lastname: Cooley
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, MA 02115, USA; Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA. Electronic address: perrimon@receptor.med.harvard.edu.
authors.forename: Norbert
authors.initials: N
authors.lastname: Perrimon
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D002455:Cell Division / Q000502:physiology*; D019070:Cell Lineage / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005854:Germ Cells / Q000166:cytology* / Q000378:metabolism; D010053:Ovary / Q000166:cytology / Q000378:metabolism; D034622:RNA Interference / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins