title: The angiogenic activity of ascites in the course of ovarian cancer as a marker of disease progression.
abstract: Ovarian cancer cells are able to create invasive implants in the peritoneum and their growth is directly associated with the angiogenetic potential. This effect is probably stimulated by vascular endothelial growth factor (VEGF) and interleukin-8 (IL-8), which are both found in ascites. The aim of this study was to assess the influence of ascites produced by ovarian cancer on the angiogenesis. Peritoneal fluid was collected from patients with advanced ovarian cancer; cancer cells were separated from CD45+ leukocytes. Angiogenesis was assessed in mice, after intradermal injection of full cellular suspension together with supernatant or phosphate buffered saline, purified cancer cells suspension, or CD45+ leukocytes suspension. The angiogenesis index (AI) was assessed after 72 hours. VEGF and Il-8 were measured in the supernatant and cellular suspension. AI was the highest in the isolated cancer cells suspensions as well in the group stimulated with supernatant. Both VEGF and IL-8 were high in supernatants from ascites rich in cancer cells (>45%). A significant correlation was revealed between IL-8 concentration and AI. We conclude that ascites in patients with advanced ovarian cancer stimulates angiogenesis and this mechanism is dependent mostly on cancer cells activity and enhanced by cooperation with infiltrating leukocytes.
authors.affiliation: Department of Gynecological Oncology and Oncology, Medicover Hospital, Rzeczypospolitej 5, 02-972 Warsaw, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Gawrychowski
authors.affiliation: Department of Obstetrics and Gynecology, Institute of Mother and Child, Kasprzaka 17A, 01-211 Warsaw, Poland ; Department of General and Experimental Pathology, Warsaw Medical University, Pawińskiego 3C, 02-106 Warsaw, Poland.
authors.forename: Grzegorz
authors.identifier: 0000-0003-4143-2777
authors.initials: G
authors.lastname: Szewczyk
authors.affiliation: Department of Pathology, Centre of Biostructure Research, Warsaw Medical University, Chałubińskiego 5, 02-004 Warsaw, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Skopińska-Różewska
authors.affiliation: Department of Applied Pharmacy and Bioengineering, Warsaw Medical University, Warsaw, Poland ; Institute of Mother and Child, Kasprzaka 17A, 01-211 Warsaw, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Małecki
authors.affiliation: 1st Department of Obstetrics and Gynecology, Warsaw Medical University, Pl. Starynkiewicza 1, 02-015 Warsaw, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Barcz
authors.affiliation: 1st Department of Obstetrics and Gynecology, Warsaw Medical University, Pl. Starynkiewicza 1, 02-015 Warsaw, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Kamiński
authors.affiliation: Department of Surgical Oncology, Holy Family Hospital, Madalińskiego 25, 02-544 Warsaw, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Miedzińska-Maciejewska
authors.affiliation: Department of Oncology, National Institute of Oncology, Wawelska 15, 02-034 Warsaw, Poland.
authors.forename: Wacław
authors.initials: W
authors.lastname: Śmiertka
authors.affiliation: Department of General and Experimental Pathology, Warsaw Medical University, Pawińskiego 3C, 02-106 Warsaw, Poland.
authors.forename: Dariusz
authors.identifier: 0000-0002-0124-060X
authors.initials: D
authors.lastname: Szukiewicz
authors.affiliation: Department of Histology and Embryology, Centre of Biostructure Research, Warsaw Medical University, Chałubińskiego 5, 02-004 Warsaw, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Skopiński
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D001202:Ascitic Fluid / Q000502:physiology*; D018450:Disease Progression; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000473:pathology*; D010051:Ovarian Neoplasms / Q000473:pathology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D016209:Interleukin-8; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A