title: Estrogen-responsive genes overlap with triiodothyronine-responsive genes in a breast carcinoma cell line.
abstract: It has been well established that estrogen plays an important role in the progression and treatment of breast cancer. However, the role of triiodothyronine (T₃) remains controversial. We have previously shown its capacity to stimulate the development of positive estrogen receptor breast carcinoma, induce the expression of genes (PR, TGF-alpha) normally stimulated by estradiol (E₂), and suppress genes (TGF-beta) normally inhibited by E₂. Since T₃ regulates growth hormones, metabolism, and differentiation, it is important to verify its action on other genes normally induced by E₂. Therefore, we used DNA microarrays to compare gene expression patterns in MCF-7 breast adenocarcinoma cells treated with E₂ and T₃. Several genes were modulated by both E₂ and T₃ in MCF-7 cells (Student's t-test, P < 0.05). Specifically, we found eight genes that were differentially expressed after treatment with both E₂ and T₃, including amphiregulin, fibulin 1, claudin 6, pericentriolar material 1, premature ovarian failure 1B, factor for adipocyte differentiation-104, sterile alpha motif domain containing 9, and likely ortholog of rat vacuole membrane protein 1 (fold change > 2.0, pFDR < 0.05). We confirmed our microarray results by real-time PCR. Our findings reveal that certain genes in MCF-7 cells can be regulated by both E₂ and T₃.
authors.affiliation: Department of Internal Medicine, Botucatu School of Medicine, University of São Paulo State (UNESP), 18618-000 Botucatu, SP, Brazil.
authors.forename: Nancy Bueno
authors.identifier: 0000-0002-8391-3248
authors.initials: NB
authors.lastname: Figueiredo
authors.affiliation: Department of Internal Medicine, Botucatu School of Medicine, University of São Paulo State (UNESP), 18618-000 Botucatu, SP, Brazil.
authors.forename: Sílvia Helena
authors.initials: SH
authors.lastname: Cestari
authors.affiliation: Department of Internal Medicine, Botucatu School of Medicine, University of São Paulo State (UNESP), 18618-000 Botucatu, SP, Brazil.
authors.forename: Sandro José
authors.initials: SJ
authors.lastname: Conde
authors.affiliation: Department of Internal Medicine, Botucatu School of Medicine, University of São Paulo State (UNESP), 18618-000 Botucatu, SP, Brazil.
authors.forename: Renata Azevedo Melo
authors.initials: RA
authors.lastname: Luvizotto
authors.affiliation: Department of Internal Medicine, Botucatu School of Medicine, University of São Paulo State (UNESP), 18618-000 Botucatu, SP, Brazil.
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: De Sibio
authors.affiliation: Department of Internal Medicine, Botucatu School of Medicine, University of São Paulo State (UNESP), 18618-000 Botucatu, SP, Brazil.
authors.forename: Denise
authors.initials: D
authors.lastname: Perone
authors.affiliation: Department of Radiology, Medicine School, University of São Paulo, USP, 01246-903 São Paulo, SP, Brazil.
authors.forename: Maria Lúcia Hirata
authors.initials: ML
authors.lastname: Katayama
authors.affiliation: Research Center, AC Camargo Hospital, 01509-900 São Paulo, SP, Brazil.
authors.forename: Dirce Maria
authors.initials: DM
authors.lastname: Carraro
authors.affiliation: Research Center, AC Camargo Hospital, 01509-900 São Paulo, SP, Brazil.
authors.forename: Helena Paula
authors.initials: HP
authors.lastname: Brentani
authors.affiliation: Research Center, AC Camargo Hospital, 01509-900 São Paulo, SP, Brazil.
authors.forename: Maria Mitzi
authors.initials: MM
authors.lastname: Brentani
authors.affiliation: Department of Internal Medicine, Botucatu School of Medicine, University of São Paulo State (UNESP), 18618-000 Botucatu, SP, Brazil ; Department of Clinical Medicine of University of São Paulo State, Rubiao Junior District, s/n, 18618-000 Botucatu, SP, Brazil.
authors.forename: Célia Regina
authors.initials: CR
authors.lastname: Nogueira
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics*; D002277:Carcinoma / Q000235:genetics*; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D061986:MCF-7 Cells; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014284:Triiodothyronine / Q000494:pharmacology*
chemical_list: D004967:Estrogens; D014284:Triiodothyronine