title: Growth conditions of clostridium perfringens type B for production of toxins used to obtain veterinary vaccines.
abstract: The diseases caused for Clostridium perfringens are generically called enterotoxemias because toxins produced in the intestine may be absorbed into the general circulation. C. perfringens type B, grown in batch fermentation, produced toxins used to obtain veterinary vaccines. Glucose in concentrations of 1.4-111.1 mM was used to define the culture medium. The minimum concentration for a satisfactory production of vaccines against clostridial diseases was 55.6 mM. Best results were brought forth by meat and casein peptones, both in the concentration 5.0 g l(-1) in combination with glucose and a culture pH maintained at 6.5 throughout the fermentation process. The production of lactic, acetic and propionic organic acids was observed. Ethanol was the metabolite produced in the highest concentration when cultures maintained steady pH of 6.5 with exception of cultures with initial glucose concentration of 1.4 mM, where the highest production was of propionic acid. Maximal cell concentration and the highest toxin title concomitantly low yield coefficient to organic acids and ethanol were obtained using basal medium containing 111.1 mM glucose under a controlled pH culture (pH) 6.5 in batch fermentations of C. perfringens type B. These data contribute to improve process for industrial toxin production allowing better condition to produce a toxoid vaccine.
authors.affiliation: Universidade Federal de Minas Gerais - Instituto de Ciências Agrárias - UFMG, Campus Regional de Montes Claros. Av.: Universitária, 1.000 - Bairro Universitário, Montes Claros, MG, CEP 39.404-547, Brazil, ibrandi@hotmail.com.
authors.forename: Igor
authors.initials: I
authors.lastname: Viana Brandi
authors.forename: Otto
authors.initials: O
authors.lastname: Domenici Mozzer
authors.forename: Edson Vander
authors.initials: EV
authors.lastname: Jorge
authors.forename: Frederico Jose
authors.initials: FJ
authors.lastname: Vieira Passos
authors.forename: Flavia Maria
authors.initials: FM
authors.lastname: Lopes Passos
authors.forename: Alex Sander Rodrigues
authors.initials: AS
authors.lastname: Cangussu
authors.forename: Eliane
authors.initials: E
authors.lastname: Macedo Sobrinho
mesh_terms: D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology*; D003016:Clostridium perfringens / Q000254:growth & development* / Q000276:immunology / Q000378:metabolism; D003470:Culture Media; D014730:Veterinary Medicine
chemical_list: D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D003470:Culture Media