title: Simultaneous production of fatty acid methyl esters and diglycerides by four recombinant Candida rugosa lipase's isozymes.
abstract: In this study, the catalytic efficiency of four recombinant CRL (Candida rugosa lipase) isozymes (LIP1-LIP4) towards the production of fatty acid methyl ester (FAME) was compared and evaluated as an alternative green method for industrial applications. The results indicated that the recombinant C. rugosa LIP1 enzyme exhibited the highest catalytic efficiency for FAME production compared to the recombinant C. rugosa LIP2-LIP4 enzymes. The optimal conditions were as follows: pH 7.0, methanol/soybean oil molar ratio: 3/1, enzyme amount: 2U (1.6 μL), reaction temperature: 20°C, 22 h of reaction time, and 3 times of methanol addition (1 mol/6h), and resulted in 61.5 ± 1.5 wt.% of FAME conversion. The reaction product contained also 10 wt.% of DAG with a ratio of 1,3-DAG to 1,2-DAG of approximately 4:6, and can be potentially used in industrial applications as a food emulsifier.
authors.affiliation: Department of Medicinal Botanicals and Health Applications, Dayeh University, Changhua 515, Taiwan. Electronic address: swchang@mail.dyu.edu.tw.
authors.forename: Shu-Wei
authors.initials: SW
authors.lastname: Chang
authors.affiliation: Department of Food Science and Biotechnology, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Myron
authors.initials: M
authors.lastname: Huang
authors.affiliation: Department of Life Science, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Yu-Hsun
authors.initials: YH
authors.lastname: Hsieh
authors.affiliation: Department of Bioindustry Technology, Dayeh University, Changhua 515, Taiwan.
authors.forename: Ying-Ting
authors.initials: YT
authors.lastname: Luo
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Tsung-Ta
authors.initials: TT
authors.lastname: Wu
authors.affiliation: Department of Nutrition, China Medical University, No. 91, Hsueh-Shih Rd., Taichung 404, Taiwan.
authors.forename: Chia-Wen
authors.initials: CW
authors.lastname: Tsai
authors.affiliation: Department of Food Science and Biotechnology, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Chin-Shuh
authors.initials: CS
authors.lastname: Chen
authors.affiliation: Department of Biological Science & Technology, I-Shou University, Kaohsiung City 84001, Taiwan; Department of Food Science and Biotechnology, National Chung Hsing University, Taichung 402, Taiwan; Department of Life Science, National Chung Hsing University, Taichung 402, Taiwan; Agricultural Biotechnology Research Center, Academia Sinica, Nankang, Taipei 11529, Taiwan; Agricultural Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan. Electronic address: shawjf@isu.edu.tw.
authors.forename: Jei-Fu
authors.initials: JF
authors.lastname: Shaw
mesh_terms: D055162:Biocatalysis; D002175:Candida / Q000201:enzymology*; D004075:Diglycerides / Q000737:chemistry*; D004952:Esters / Q000737:chemistry*; D005227:Fatty Acids / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics; D008049:Lipase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D004075:Diglycerides; D004952:Esters; D005227:Fatty Acids; D005656:Fungal Proteins; D007527:Isoenzymes; D011994:Recombinant Proteins; D008049:Lipase
keywords: Candida rugosa lipase; Diacylglycerol; Emulsifier; Esterification; Fatty acid methyl ester; Isozymes