title: Determination of the aflatoxin AFB1 from corn by direct analysis in real time-mass spectrometry (DART-MS).
abstract: Direct analysis in real time (DART) ionisation coupled to a high-resolution mass spectrometer (MS) was used for screening of aflatoxins from a variety of surfaces and the rapid quantitative analysis of a common form of aflatoxin, AFB1, extracted from corn. Sample preparation procedure and instrument parameter settings were optimised to obtain sensitive and accurate determination of aflatoxin AFB1. 84:16 acetonitrile water extracts of corn were analysed by DART-MS. The lowest calibration level (LCL) for aflatoxin AFB1 was 4 μg kg⁻¹. Quantitative analysis was performed with the use of matrix-matched standards employing the ¹³C-labelled internal standard for AFB1. DART-MS of spiked corn extracts gave linear response in the range 4-1000 μg kg⁻¹. Good recoveries (94-110%) and repeatabilities (RSD = 0.7-6.9%) were obtained at spiking levels of 20 and 100 μg kg⁻¹ with the use of an isotope dilution technique. Trueness of data obtained for AFB1 in maize by DART-MS was demonstrated by analysis of corn certified reference materials.
authors.affiliation: a Bacterial Foodborne Pathogen and Mycology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service , US Department of Agriculture , Peoria , IL , USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Busman
authors.forename: Jihong
authors.initials: J
authors.lastname: Liu
authors.forename: Hongjian
authors.initials: H
authors.lastname: Zhong
authors.forename: John R
authors.initials: JR
authors.lastname: Bobell
authors.forename: Chris M
authors.initials: CM
authors.lastname: Maragos
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis* / Q000737:chemistry; D002138:Calibration; D002273:Carcinogens / Q000032:analysis* / Q000737:chemistry; D002681:China; D002855:Chromatography, Thin Layer; D005506:Food Contamination; D005513:Food Inspection / Q000379:methods*; D005898:Glass; D006358:Hot Temperature; D057230:Limit of Detection; D013058:Mass Spectrometry; D010209:Paper; D010936:Plant Extracts / Q000737:chemistry; D011042:Poisons / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry*; D058428:Silica Gel; D014835:Volatilization; D003313:Zea mays / Q000737:chemistry*
chemical_list: D002273:Carcinogens; D010936:Plant Extracts; D011042:Poisons; D058428:Silica Gel; D016604:Aflatoxin B1
keywords: aflatoxin; mass spectrometry; mycotoxin