title: Screening proteins for NMR suitability.
abstract: NMR spectroscopy is a valuable tool in structural genomics. Identification of protein samples that are amenable to structure determination by NMR spectroscopy requires efficient screening. The preparation of multiple samples in parallel and screening by NMR is described. The method described is applicable to large structural genomics projects but can easily be scaled down for application to small structural biology projects. All the equipment used is commonly found in any NMR structural biology laboratory.
authors.affiliation: Division of Cancer Genomics and Proteomics, and Northeast Structural Genomics Consortium (NESG), Ontario Cancer Institute, Toronto, ON, Canada.
authors.forename: Adelinda A
authors.initials: AA
authors.lastname: Yee
authors.forename: Anthony
authors.initials: A
authors.lastname: Semesi
authors.forename: Maite
authors.initials: M
authors.lastname: Garcia
authors.forename: Cheryl H
authors.initials: CH
authors.lastname: Arrowsmith
mesh_terms: D018360:Crystallography, X-Ray; D023281:Genomics; D009682:Magnetic Resonance Spectroscopy; D008967:Molecular Biology / Q000379:methods*; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins