title: Development and Validation of a HPTLC Method for Simultaneous Estimation of L-Glutamic Acid and γ-Aminobutyric Acid in Mice Brain.
abstract: A new robust, simple and economic high performance thin layer chromatographic method was developed for simultaneous estimation of L-glutamic acid and γ-amino butyric acid in brain homogenate. The high performance thin layer chromatographic separation of these amino acid was achieved using n-butanol:glacial acetic acid:water (22:3:5 v/v/v) as mobile phase and ninhydrin as a derivatising agent. Quantitation of the method was achieved by densitometric method at 550 nm over the concentration range of 10-100 ng/spot. This method showed good separation of amino acids in the brain homogenate with Rf value of L-glutamic acid and γ-amino butyric acid as 21.67±0.58 and 33.67±0.58, respectively. The limit of detection and limit of quantification for L-glutamic acid was found to be 10 and 20 ng and for γ-amino butyric acid it was 4 and 10 ng, respectively. The method was also validated in terms of accuracy, precision and repeatability. The developed method was found to be precise and accurate with good reproducibility and shows promising applicability for studying pathological status of disease and therapeutic significance of drug treatment.
authors.affiliation: Departments of Pharmaceutical Science and Technology, Insitute of Chemical Technology, N. P. Marg, Matunga, Mumbai-400 019, India.
authors.forename: J S
authors.initials: JS
authors.lastname: Sancheti
authors.affiliation: Department of Neuroscience, Brain Research Institute, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Sunway Campus, Petaling Jaya, Selangor 46150, Malaysia.
authors.forename: M F
authors.initials: MF
authors.lastname: Shaikh
authors.affiliation: Departments of Pharmacognosy and Phytochemistry, Bombay College of Pharmacy, Kalina, Santacruz (E), Mumbai-400 098, India.
authors.forename: P F
authors.initials: PF
authors.lastname: Khatwani
authors.affiliation: Departments of Pharmacognosy and Phytochemistry, Bombay College of Pharmacy, Kalina, Santacruz (E), Mumbai-400 098, India.
authors.forename: Savita R
authors.initials: SR
authors.lastname: Kulkarni
authors.affiliation: Departments of Pharmaceutical Science and Technology, Insitute of Chemical Technology, N. P. Marg, Matunga, Mumbai-400 019, India.
authors.forename: Sadhana
authors.initials: S
authors.lastname: Sathaye
keywords: GABA; HPTLC method; L-glutamic acid; excitatory and inhibitory amino acids; neurological disorder