title: Anti-diabetic effect of a combination of andrographolide-enriched extract of Andrographis paniculata (Burm f.) Nees and asiaticoside-enriched extract of Centella asiatica L. in high fructose-fat fed rats.
abstract: Traditionally, a combination of medicinal plants is commonly used for lowering blood glucose in diabetic patients in order to provide additional benefits of the single drug. A. paniculata and C. asiatica are two traditional medicines form South Asian and Southeast Asain countries consumed by people for treating daibates mellitus and its complications. Hyperglycemia in the rats was stimulated by high fructose-fat diet that consists of 36% fructose, 15% lard, and 5% egg yolks in 0.36 g/200 g body weight for 70 days. The rats were orally administered with the combination of andrographolide-enriched extract of A. paniculata (AEEAP) leaves and asiaticoside-enriched extract of C. asiatica (AEECA) herbs from day 70 for 7 days. Antidiabetic activity was evaluated by estimating mainly the blood glucose levels and other parameters such as HDL, LDL, cholesterol and triglyceride. The results showed that combination at the ratio of 70:30 exhibited a promosing antidiabetic effect in high-fat-fructose-fed rat, and exhibited sinergistic effects on blood cholesterol and HDL levels. It can be concluded that its antidiabetic effect was better than that of single treatment of AEEAP or AEECA. That combination was also potential to develop as a blood glucose-lowering agent for diabetic patients.
authors.affiliation: Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Jogjakarta, Indonesia, 55281. agungendronugroho@yahoo.com
authors.forename: Agung Endro
authors.initials: AE
authors.lastname: Nugroho
authors.affiliation: Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Jogjakarta, Indonesia, 55281.
authors.forename: Novena Yety
authors.initials: NY
authors.lastname: Lindawati
authors.affiliation: Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Jogjakarta, Indonesia, 55281.
authors.forename: Kyky
authors.initials: K
authors.lastname: Herlyanti
authors.affiliation: Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Jogjakarta, Indonesia, 55281.
authors.forename: Lina
authors.initials: L
authors.lastname: Widyastuti
authors.affiliation: Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Jogjakarta, Indonesia, 55281.
authors.forename: Suwidjiyo
authors.initials: S
authors.lastname: Pramono
mesh_terms: D000089002:Andrographis paniculata; D000818:Animals; D028041:Centella / Q000737:chemistry; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000473:pathology; D059305:Diet, High-Fat; D004224:Diterpenes / Q000008:administration & dosage; D004338:Drug Combinations; D004357:Drug Synergism; D005632:Fructose / Q000008:administration & dosage; D006801:Humans; D007004:Hypoglycemic Agents / Q000008:administration & dosage; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D051381:Rats; D014315:Triterpenes / Q000008:administration & dosage
chemical_list: D004224:Diterpenes; D004338:Drug Combinations; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D014315:Triterpenes; C000713167:Andrographis paniculata extract; D005632:Fructose; C030419:andrographolide; C004446:asiaticoside