title: Rpi-blb2-mediated late blight resistance in Nicotiana benthamiana requires SGT1 and salicylic acid-mediated signaling but not RAR1 or HSP90.
abstract: The Rpi-blb2 recognizes the presence of the Phytophthora infestans AVRblb2 and initiates effector-triggered immunity (ETI). We performed gain-of-function and loss-of-function studies in Nicotiana benthamiana to elucidate Rpi-blb2-mediated resistance to P. infestans. Rpi-blb2 triggered a hypersensitive response through SGT1-mediated, but not RAR-mediated or HSP90-mediated, pathways. NbSGT1 was also required for basal and ETI-mediated by Rpi-blb2 in N. benthamiana. Moreover, salicylic acid (SA) affected basal defense and Rpi-blb2-mediated resistance against P. infestans. The increased susceptibility of Rpi-blb2-transgenic plants in the NahG-background correlated with reduced levels of SA. These findings provide evidence for the roles of SGT1- and SA-signaling in Rpi-blb2-mediated resistance against P. infestans.
authors.affiliation: Department of Plant Science, Plant Genomics and Breeding Institute, Institute of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Republic of Korea; Korea Research Institute of Bioscience and Biotechnology, and Cabbage Genomics Assisted Breeding Supporting Center, 111, Gwahangno, Yuseong-gu, Deajeon 305-806, Republic of Korea.
authors.forename: Sang-Keun
authors.initials: SK
authors.lastname: Oh
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, and Cabbage Genomics Assisted Breeding Supporting Center, 111, Gwahangno, Yuseong-gu, Deajeon 305-806, Republic of Korea. Electronic address: kimhr@kribb.re.kr.
authors.forename: HyeRan
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Plant Science, Plant Genomics and Breeding Institute, Institute of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Republic of Korea. Electronic address: doil@snu.ac.kr.
authors.forename: Doil
authors.initials: D
authors.lastname: Choi
mesh_terms: D001426:Bacterial Proteins / Q000502:physiology; D060467:Disease Resistance; D005964:Glucosyltransferases / Q000378:metabolism*; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D055750:Phytophthora infestans / Q000502:physiology*; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology; D018515:Plant Leaves / Q000201:enzymology / Q000276:immunology / Q000382:microbiology; D010940:Plant Proteins / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000276:immunology / Q000382:microbiology; D020156:Salicylic Acid / Q000378:metabolism*; D015398:Signal Transduction; D014026:Nicotiana / Q000201:enzymology* / Q000276:immunology / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D018841:HSP90 Heat-Shock Proteins; D010940:Plant Proteins; D006861:Hydrogen Peroxide; D005964:Glucosyltransferases; D020156:Salicylic Acid
keywords: AVRblb2; Nicotiana benthamiana; Phytophthora infestans; Resistance; Rpi-blb2; SGT1; Salicylic acid