title: Acute and subacute pulmonary toxicity caused by a single intratracheal instillation of colloidal silver nanoparticles in mice: pathobiological changes and metallothionein responses.
abstract: To study the acute and subacute pulmonary toxicity of colloidal silver nanoparticles (Ag-NPs), 0 or 100 ppm of Ag-NPs were instilled intratracheally in mice. Cellular and biochemical parameters in bronchoalveolar lavage fluid (BALF) and histological alterations were determined 1, 3, 7, 15, and 30 days after instillation. Ag-NPs induced moderate pulmonary inflammation and injury on BALF indices during the acute period; however, these changes gradually regressed in a time-dependent manner. Concomitant histopathological and laminin immunohistochemical findings generally correlated to BALF data. Superoxide dismutase and metallothionein expression occurred in particle-laden macrophages and alveolar epithelial cells, which correlated to lung lesions in mice treated with Ag-NPs. These findings suggest that instillation of Ag-NPs causes transient moderate acute lung inflammation and tissue damage. Oxidative stress may underlie the induction of injury to lung tissue. Moreover, the expression of metallothionein in tissues indicated the protective response to exposure to Ag-NPs.
authors.affiliation: Department of Veterinary Pathology, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Theerayuth
authors.initials: T
authors.lastname: Kaewamatawong
authors.affiliation: Department of Veterinary Pathology, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Wijit
authors.initials: W
authors.lastname: Banlunara
authors.affiliation: Sensor Research Unit, Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Pattwat
authors.initials: P
authors.lastname: Maneewattanapinyo
authors.affiliation: Sensor Research Unit, Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Chuchaat
authors.initials: C
authors.lastname: Thammachareon
authors.affiliation: Sensor Research Unit, Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Sanong
authors.initials: S
authors.lastname: Ekgasit
mesh_terms: D000208:Acute Disease; D000280:Administration, Inhalation; D000818:Animals; D003102:Colloids; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008171:Lung Diseases / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000633:toxicity*; D008668:Metallothionein / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D012834:Silver / Q000008:administration & dosage / Q000633:toxicity*; D013997:Time Factors
chemical_list: D003102:Colloids; D012834:Silver; D008668:Metallothionein