title: Three dimensional microfluidic cell arrays for ex vivo drug screening with mimicked vascular flow.
abstract: Currently, there are no reliable ex vivo models that predict anticancer drug responses in human tumors accurately. A comprehensive method of mimicking a 3D microenvironment to study effects of anticancer drugs on specific cancer types is essential. Here, we report the development of a three-dimensional microfluidic cell array (3D μFCA), which reconstructs a 3D tumor microenvironment with cancer cells and microvascular endothelial cells. To mimic the in vivo spatial relationship between microvessels and nonendothelial cells embedded in extracellular matrix, three polydimethylsiloxane (PDMS) layers were built into this array. The multilayer property of the device enabled the imitation of the drug delivery in a microtissue array with simulated blood circulation. This 3D μFCA system may provide better predictions of drug responses and identification of a suitable treatment for a specific patient if biopsy samples are used. To the pharmaceutical industry, the scaling-up of our 3D μFCA system may offer a novel high throughput screening tool.
authors.affiliation: Department of Biomedical Engineering, The City College of the City University of New York , 160 Convent Ave. Steinman Hall T-434, New York, New York 10031, United States.
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Dereli-Korkut
authors.forename: H Dogus
authors.initials: HD
authors.lastname: Akaydin
authors.forename: A H Rezwanuddin
authors.initials: AH
authors.lastname: Ahmed
authors.forename: Xuejun
authors.initials: X
authors.lastname: Jiang
authors.forename: Sihong
authors.initials: S
authors.lastname: Wang
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D044085:Microfluidics; D008855:Microscopy, Electron, Scanning; D018716:Molecular Mimicry; D059016:Tumor Microenvironment
chemical_list: D000970:Antineoplastic Agents