title: Mouse dipeptidyl peptidase 4 is not a functional receptor for Middle East respiratory syndrome coronavirus infection.
abstract: Human dipeptidyl peptidase 4 (hDPP4) was recently identified as the receptor for Middle East respiratory syndrome coronavirus (MERS-CoV) infection, suggesting that other mammalian DPP4 orthologs may also support infection. We demonstrate that mouse DPP4 cannot support MERS-CoV infection. However, employing mouse DPP4 as a scaffold, we identified two critical amino acids (A288L and T330R) that regulate species specificity in the mouse. This knowledge can support the rational design of a mouse-adapted MERS-CoV for rapid assessment of therapeutics.
authors.affiliation: Department of Genetics, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA.
authors.forename: Adam S
authors.initials: AS
authors.lastname: Cockrell
authors.forename: Kayla M
authors.initials: KM
authors.lastname: Peck
authors.forename: Boyd L
authors.initials: BL
authors.lastname: Yount
authors.forename: Sudhakar S
authors.initials: SS
authors.lastname: Agnihothram
authors.forename: Trevor
authors.initials: T
authors.lastname: Scobey
authors.forename: Nicole R
authors.initials: NR
authors.lastname: Curnes
authors.forename: Ralph S
authors.initials: RS
authors.lastname: Baric
authors.forename: Mark T
authors.initials: MT
authors.lastname: Heise
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017934:Coronavirus / Q000502:physiology*; D018352:Coronavirus Infections / Q000821:virology; D004252:DNA Mutational Analysis; D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism*; D058507:Host Specificity; D006801:Humans; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011991:Receptors, Virus / Q000378:metabolism*; D013045:Species Specificity; D053585:Virus Attachment
chemical_list: D011991:Receptors, Virus; D018819:Dipeptidyl Peptidase 4