title: Polyoxometalate@magnetic graphene as versatile immobilization matrix of Ru(bpy)3(2+) for sensitive magneto-controlled electrochemiluminescence sensor and its application in biosensing.
abstract: We demonstrated here the exploration of polyoxometalate (POM) coated magnetic Fe3O4/reduced graphene oxide (POM@mrGO) composite as the versatile immobilization matrix for the electrochemiluminescence (ECL) agent Ru(bpy)3(2+). The effective modification of Ru(bpy)3(2+)/POM@mrGO hybrid simply involved using magnetic electrode showed 10-fold ECL intensity increase than that observed for Ru(bpy)3(2+)/Nafion@mrGO to the same concentration of nicotinamide adenine dinucleotide (NADH), which is largely due to POM׳s good electrocatalytic activity towards NADH oxidation. These findings allowed the stable and ultrasensitive ECL detection of NADH as low as 0.1 nM. The good stability and high sensitivity of the magneto-controlled ECL sensor enabled us to explore the feasibility of applying the sensing platform to fabricating the ECL biosensors in which the NADH was produced from the dehydrogenase-based enzymatic reaction in the presence of NAD(+) cofactor. With L-lactate dehydrogenase as a model, a L-lactate biosensor was successfully constructed where we showed that the ECL intensity of the biosensor increased with the increasing L-lactate concentration. Excellent performance of the presented biosensor has been achieved including a wide linear range extended from 5.0×10(-9) M to 5.0×10(-4) M and an extremely low detection limit of 0.4 nM. Such sensing strategy combines enzymatic selectivity with simple sensor preparation can be used as a new and biocompatible platform for dehydrogenase-based ECL biosensing.
authors.affiliation: Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Qian
authors.affiliation: Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China. Electronic address: wangkun@ujs.edu.cn.
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.affiliation: Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Yicong
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Xingwang
authors.initials: X
authors.lastname: Yang
authors.affiliation: Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Xiaoya
authors.initials: X
authors.lastname: Dong
authors.affiliation: Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Huaming
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Baijing
authors.initials: B
authors.lastname: Qiu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D055664:Electrochemical Techniques / Q000379:methods; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods*; D060328:Magnetic Phenomena; D059346:Magnets / Q000737:chemistry*; D009243:NAD / Q000032:analysis*; D009942:Organometallic Compounds / Q000737:chemistry*; D017973:Tungsten Compounds / Q000737:chemistry*; D000099294:Polyoxometalates
chemical_list: D006108:Graphite; D009243:NAD; D009942:Organometallic Compounds; D017973:Tungsten Compounds; C102296:polyoxometalate I; D000099294:Polyoxometalates; C415768:tris(2,2'-bipyridyl)ruthenium(II)
keywords: Electrochemiluminescence; Magnetic graphene; NADH; Polyoxometalate; Ru(bpy)(3)(2+); l-Lactate