title: Ligand screening using NMR.
abstract: NMR has proven to be an invaluable technique for identifying and characterizing ligand interactions with biomolecules. NMR-based detection of ligand binding to protein targets is described. Specifically, the use of the WaterLOGSY NMR experiment to screen mixtures of compounds from a fragment library for binding to influenza H5 hemagglutinin is detailed.
authors.affiliation: Center for Structural Biology, University of Illinois at Chicago, 1100 S. Ashland Ave, Chicago, IL, 60607, USA.
authors.forename: Benjamin E
authors.initials: BE
authors.lastname: Ramirez
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Antanasijevic
authors.forename: Michael
authors.initials: M
authors.lastname: Caffrey
mesh_terms: D004353:Drug Evaluation, Preclinical; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry; D006801:Humans; D007251:Influenza, Human / Q000235:genetics / Q000378:metabolism*; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D008967:Molecular Biology / Q000379:methods*; D011485:Protein Binding; D014867:Water / Q000737:chemistry
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D008024:Ligands; C109963:hemagglutinin, avian influenza A virus; D014867:Water