title: Suppression of autophagy by chloroquine sensitizes 5-fluorouracil-mediated cell death in gallbladder carcinoma cells.
abstract: Autophagy1 is a complex of adaptive cellular response that enhances cancer cell survival in the face of cellular stresses such as chemothery. Here we show that in human gallbladder carcinoma (GBC) cells lines, SGC-996 and GBC-SD, autophagy is induced by the DNA damaging agent 5-fluorouracil (5-FU). While in combination with the pre-treatment of chloroquine (CQ), a inhibitor of autophagy, the inhibition of 5-FU to the proliferation and viability of GBC cells was potentiated. Furthermore, 5-FU treatment resulted in a general increase of the apoptotic rate and G0/G1 arrest of GBC cells, and the effect was potentiated by CQ pre-treatment. Since 5-FU induced autophagy in GBC cells, and CQ inhibited autophagy, our findings suggest a possible mechanism that CQ inhibited 5-FU-induced autophagy, which modified the cytotoxicity of 5-FU. The combination therapy of CQ and 5-FU should be considered as an effective strategy for the treatment of gallbladder carcinoma.
authors.affiliation: Key Lab of Surgery of Zhejiang province, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou 310016, People's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Liang
authors.affiliation: Key Lab of Surgery of Zhejiang province, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou 310016, People's Republic of China.
authors.forename: JiaCheng
authors.initials: J
authors.lastname: Tang
authors.affiliation: Key Lab of Surgery of Zhejiang province, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou 310016, People's Republic of China.
authors.forename: YueLong
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Key Lab of Surgery of Zhejiang province, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou 310016, People's Republic of China.
authors.forename: RenAn
authors.initials: R
authors.lastname: Jin
authors.affiliation: Key Lab of Surgery of Zhejiang province, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou 310016, People's Republic of China.
authors.forename: XiuJun
authors.initials: X
authors.lastname: Cai