title: Branched amphiphilic peptide capsules: cellular uptake and retention of encapsulated solutes.
abstract: Branched amphiphilic peptide capsules (BAPCs) are peptide nano-spheres comprised of equimolar proportions of two branched peptide sequences bis(FLIVI)-K-KKKK and bis(FLIVIGSII)-K-KKKK that self-assemble to form bilayer delimited capsules. In two recent publications we described the lipid analogous characteristics of our BAPCs, examined their initial assembly, mode of fusion, solute encapsulation, and resizing and delineated their capability to be maintained at a specific size by storing them at 4°C. In this report we describe the stability, size limitations of encapsulation, cellular localization, retention and, bio-distribution of the BAPCs in vivo. The ability of our constructs to retain alpha particle emitting radionuclides without any apparent leakage and their persistence in the peri-nuclear region of the cell for extended periods of time, coupled with their ease of preparation and potential tune-ability, makes them attractive as biocompatible carriers for targeted cancer therapy using particle emitting radioisotopes. This article is part of a Special Issue entitled: Interfacially Active Peptides and Proteins. Guest Editors: William C. Wimley and Kalina Hristova.
authors.affiliation: Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, KS 66502, USA.
authors.forename: Pinakin
authors.initials: P
authors.lastname: Sukthankar
authors.affiliation: Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, KS 66502, USA.
authors.forename: L Adriana
authors.initials: LA
authors.lastname: Avila
authors.affiliation: Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, KS 66502, USA.
authors.forename: Susan K
authors.initials: SK
authors.lastname: Whitaker
authors.affiliation: Division of Biochemistry, Core Research Facilities, Jikei University School of Medicine, Tokyo 105-8461, Japan.
authors.forename: Takeo
authors.initials: T
authors.lastname: Iwamoto
authors.affiliation: European Commission, Joint Research Centre, Institute for Transuranium Elements, P.O. Box 2340, D-76125 Karlsruhe, Germany.
authors.forename: Alfred
authors.initials: A
authors.lastname: Morgenstern
authors.affiliation: European Commission, Joint Research Centre, Institute for Transuranium Elements, P.O. Box 2340, D-76125 Karlsruhe, Germany.
authors.forename: Christos
authors.initials: C
authors.lastname: Apostolidis
authors.affiliation: Department of Medicinal Chemistry, University of Kansas, Lawrence, KS 66045-7582, USA.
authors.forename: Ke
authors.initials: K
authors.lastname: Liu
authors.affiliation: Department of Medicinal Chemistry, University of Kansas, Lawrence, KS 66045-7582, USA.
authors.forename: Robert P
authors.initials: RP
authors.lastname: Hanzlik
authors.affiliation: Department of Radiology, Albert Einstein College of Medicine, 1695A Eastchester Rd., Bronx, NY 10461, USA.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Dadachova
authors.affiliation: Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, KS 66502, USA. Electronic address: jtomich@ksu.edu.
authors.forename: John M
authors.initials: JM
authors.lastname: Tomich
mesh_terms: D000186:Actinium / Q000627:therapeutic use; D002214:Capsules / Q000737:chemistry; D016503:Drug Delivery Systems; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry* / Q000627:therapeutic use; D054118:Nanospheres / Q000737:chemistry / Q000627:therapeutic use; D009369:Neoplasms / Q000188:drug therapy; D010316:Particle Size; D010455:Peptides / Q000737:chemistry* / Q000627:therapeutic use; D011868:Radioisotopes / Q000627:therapeutic use; D012996:Solutions
chemical_list: D002214:Capsules; D008051:Lipid Bilayers; D008081:Liposomes; D010455:Peptides; D011868:Radioisotopes; D012996:Solutions; D000186:Actinium
keywords: (225)Actinium; Alpha particle therapy; BAPC; Nano-capsule; Peptide capsule; Self-assembling peptide