title: [Recent advances in the techniques of protein-protein interaction study].
abstract: Protein-protein interactions play key roles in the development of organisms and the response to biotic and abiotic stresses. Several wet-lab methods have been developed to study this challenging area,including yeast two-hybrid system, tandem affinity purification, Co-immunoprecipitation, GST Pull-down, bimolecular fluorescence complementation, fluorescence resonance energy transfer and surface plasmon resonance analysis. In this review, we discuss theoretical principles and relative advantages and disvantages of these techniques,with an emphasis on recent advances to compensate for limitations.
authors.affiliation: Institute of Genetics, College of Life Sciences, Zhejiang University, Hangzhou 310058, China. wmq@zju.edu.cn
authors.forename: Ming-Qiang
authors.initials: MQ
authors.lastname: Wang
authors.affiliation: Institute of Genetics, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Jin-Xia
authors.initials: JX
authors.lastname: Wu
authors.affiliation: Tibet Academy of Agricultural and Animal Hushandry sciences, Lhasa 850000, China.
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Zhang
authors.affiliation: Institute of Genetics, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Han
authors.affiliation: Institute of Genetics, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Hong-Wu
authors.initials: HW
authors.lastname: Bian
authors.affiliation: Institute of Genetics, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Mu-Yuan
authors.initials: MY
authors.lastname: Zhu
mesh_terms: D000818:Animals; D006801:Humans; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods / Q000639:trends*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D011506:Proteins