title: Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP).
abstract: We report a one-pot, closed-vessel enzymatic assay that eliminates carryover contamination while preserving robust DNA amplification in loop-mediated isothermal amplification (LAMP), providing reliable and rapid detection of target DNA in contaminated samples.
authors.affiliation: Department of Mechanical Engineering, University of California, Santa Barbara, CA 93106, USA. tsoh@engr.ucsb.edu.
authors.forename: Kuangwen
authors.initials: K
authors.lastname: Hsieh
authors.forename: Peter L
authors.initials: PL
authors.lastname: Mage
authors.forename: Andrew T
authors.initials: AT
authors.lastname: Csordas
authors.forename: Michael
authors.initials: M
authors.lastname: Eisenstein
authors.forename: H Tom
authors.initials: HT
authors.lastname: Soh
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis* / Q000737:chemistry; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012486:Salmonella typhimurium / Q000235:genetics; D051981:Uracil-DNA Glycosidase / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D051981:Uracil-DNA Glycosidase