title: Stabilizing effect of small concentrations of PAMAM dendrimers at the insulin aggregation.
abstract: Dendrimers' action on proteins and peptides has a dual and controversial character. On one hand, they dissolve prion protein and amyloid fibrils aggregates, which are otherwise only soluble in solvents containing both detergents and high denaturant concentrations. On the other hand they are able to destabilize proteins in generation dependent manner. In present work we estimated the influence of small concentrations (up to 1.4 μg/ml) of cationic, neutral and anionic poly(amidoamine) dendrimers of 3rd and 4th generations on dithiotreitol induced aggregation of insulin. It was found that cationic dendrimers decreased the insulin aggregation, while anionic and neutral ones did not. At the same time, destabilizing effect of dendrimers on insulin structure was not observed. The conclusion was made that small concentrations of dendrimers can be applied to prevent or decrease the formation of misfolded structures of protein.
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., 90-236 Lodz, Poland.
authors.forename: Olga
authors.initials: O
authors.lastname: Nowacka
authors.affiliation: Institute of Biophysics and Cell Engineering of NASB, Akademicheskaja Str., 27, 220072 Minsk, Belarus. Electronic address: d.shcharbin@mail.ru.
authors.forename: Dzmitry
authors.initials: D
authors.lastname: Shcharbin
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., 90-236 Lodz, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Klajnert-Maculewicz
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., 90-236 Lodz, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Bryszewska
mesh_terms: D000818:Animals; D002417:Cattle; D050091:Dendrimers / Q000737:chemistry*; D007328:Insulin / Q000737:chemistry*; D008958:Models, Molecular
chemical_list: D050091:Dendrimers; D007328:Insulin; C104700:PAMAM Starburst
keywords: ANS; Aggregation; Circular dichroism; Dendrimer; Fluorescence; Insulin