title: Bio-barcode gel assay for microRNA.
abstract: MicroRNA has been identified as a potential biomarker because expression level of microRNA is correlated with various cancers. Its detection at low concentrations would be highly beneficial for cancer diagnosis. Here, we develop a new type of a DNA-modified gold nanoparticle-based bio-barcode assay that uses a conventional gel electrophoresis platform and potassium cyanide chemistry and show this assay can detect microRNA at aM levels without enzymatic amplification. It is also shown that single-base-mismatched microRNA can be differentiated from perfectly matched microRNA and the multiplexed detection of various combinations of microRNA sequences is possible with this approach. Finally, differently expressed microRNA levels are selectively detected from cancer cells using the bio-barcode gel assay, and the results are compared with conventional polymerase chain reaction-based results. The method and results shown herein pave the way for practical use of a conventional gel electrophoresis for detecting biomolecules of interest even at aM level without polymerase chain reaction amplification.
authors.affiliation: 1] Department of Chemistry, Seoul National University, Seoul 151-747, South Korea [2].
authors.forename: Hyojin
authors.initials: H
authors.lastname: Lee
authors.affiliation: 1] Department of Chemistry, Seoul National University, Seoul 151-747, South Korea [2].
authors.forename: Jeong-Eun
authors.initials: JE
authors.lastname: Park
authors.affiliation: Department of Chemistry, Seoul National University, Seoul 151-747, South Korea.
authors.forename: Jwa-Min
authors.initials: JM
authors.lastname: Nam
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D006046:Gold / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D011190:Potassium Cyanide / Q000737:chemistry; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D015342:DNA Probes; D006046:Gold; D035683:MicroRNAs; D011190:Potassium Cyanide; C524942:MIRN155 microRNA, human; C534425:MIR205, human; C524940:MIRN21 microRNA, human