title: Molecular inversion probe-based SPR biosensing for specific, label-free and real-time detection of regional DNA methylation.
abstract: DNA methylation has the potential to be a clinically important biomarker in cancer. This communication reports a real-time and label-free biosensing strategy for DNA methylation detection in the cancer cell line. This has been achieved by using surface plasmon resonance biosensing combined with the highly specific molecular inversion probe based amplification method, which requires only 50 ng of bisulfite treated genomic DNA.
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Laura G
authors.initials: LG
authors.lastname: Carrascosa
authors.forename: Abu Ali Ibn
authors.initials: AA
authors.lastname: Sina
authors.forename: Ramkumar
authors.initials: R
authors.lastname: Palanisamy
authors.forename: Borja
authors.initials: B
authors.lastname: Sepulveda
authors.forename: Marinus A
authors.initials: MA
authors.lastname: Otte
authors.forename: Sakandar
authors.initials: S
authors.lastname: Rauf
authors.forename: Muhamad J A
authors.initials: MJ
authors.lastname: Shiddiky
authors.forename: Matt
authors.initials: M
authors.lastname: Trau
mesh_terms: D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry; D019175:DNA Methylation; D006801:Humans; D061986:MCF-7 Cells; D015335:Molecular Probes; D013447:Sulfites / Q000737:chemistry; D020349:Surface Plasmon Resonance
chemical_list: D015335:Molecular Probes; D013447:Sulfites; D004247:DNA; C042345:hydrogen sulfite