title: In vivo X-ray cine-tomography for tracking morphological dynamics.
abstract: Scientific cinematography using ultrafast optical imaging is a common tool to study motion. In opaque organisms or structures, X-ray radiography captures sequences of 2D projections to visualize morphological dynamics, but for many applications full four-dimensional (4D) spatiotemporal information is highly desirable. We introduce in vivo X-ray cine-tomography as a 4D imaging technique developed to study real-time dynamics in small living organisms with micrometer spatial resolution and subsecond time resolution. The method enables insights into the physiology of small animals by tracking the 4D morphological dynamics of minute anatomical features as demonstrated in this work by the analysis of fast-moving screw-and-nut-type weevil hip joints. The presented method can be applied to a broad range of biological specimens and biotechnological processes.
authors.affiliation: ANKA/Institute for Photon Science and Synchrotron Radiation, Karlsruhe Institute of Technology, D-76344 Eggenstein-Leopoldshafen, Germany.
authors.forename: Tomy
authors.initials: T
authors.lastname: dos Santos Rolo
authors.forename: Alexey
authors.initials: A
authors.lastname: Ershov
authors.forename: Thomas
authors.initials: T
authors.lastname: van de Kamp
authors.forename: Tilo
authors.initials: T
authors.lastname: Baumbach
mesh_terms: D000818:Animals; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007596:Joints / Q000502:physiology / Q000648:ultrastructure; D014057:Tomography, X-Ray Computed / Q000379:methods*; D048889:Weevils / Q000502:physiology / Q000648:ultrastructure; D055114:X-Ray Microtomography / Q000379:methods*
keywords: in vivo imaging; motion tracking; screw joint; synchrotron