title: Molecular divergence and species delimitation of the cultivated oyster mushrooms: integration of IGS1 and ITS.
abstract: Identification of edible mushrooms particularly Pleurotus genus has been restricted due to various obstacles. The present study attempted to use the combination of two variable regions of IGS1 and ITS for classifying the economically cultivated Pleurotus species. Integration of the two regions proved a high ability that not only could clearly distinguish the species but also served sufficient intraspecies variation. Phylogenetic tree (IGS1+ITS) showed seven distinct clades, each clade belonging to a separate species group. Moreover, the species differentiation was tested by AMOVA and the results were reconfirmed by presenting appropriate amounts of divergence (91.82% among and 8.18% within the species). In spite of achieving a proper classification of species by combination of IGS1 and ITS sequences, the phylogenetic tree showed the misclassification of the species of P. nebrodensis and P. eryngii var. ferulae with other strains of P. eryngii. However, the constructed median joining (MJ) network could not only differentiate between these species but also offer a profound perception of the species' evolutionary process. Eventually, due to the sufficient variation among and within species, distinct sequences, simple amplification, and location between ideal conserved ribosomal genes, the integration of IGS1 and ITS sequences is recommended as a desirable DNA barcode.
authors.affiliation: Mushroom Research Centre (MRC), University of Malaya, 50603 Kuala Lumpur, Malaysia ; Division of Biotechnology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Farhat Ahmadi
authors.identifier: 0000-0002-4323-1058
authors.initials: FA
authors.lastname: Avin
authors.affiliation: Mushroom Research Centre (MRC), University of Malaya, 50603 Kuala Lumpur, Malaysia ; Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Subha
authors.initials: S
authors.lastname: Bhassu
authors.affiliation: Mushroom Research Centre (MRC), University of Malaya, 50603 Kuala Lumpur, Malaysia ; Division of Biotechnology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Yee Shin
authors.initials: YS
authors.lastname: Tan
authors.affiliation: Division of Biotechnology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Pedram
authors.identifier: 0000-0003-0750-399X
authors.initials: P
authors.lastname: Shahbazi
authors.affiliation: Mushroom Research Centre (MRC), University of Malaya, 50603 Kuala Lumpur, Malaysia ; Division of Biotechnology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Sabaratnam
authors.initials: S
authors.lastname: Vikineswary
mesh_terms: D001665:Binding Sites / Q000502:physiology; D019143:Evolution, Molecular; D010802:Phylogeny; D020076:Pleurotus / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D013045:Species Specificity