title: c-fos and c-jun expression in the liver of silver carp and the effect of microcystins.
abstract: In the present study, c-fos and c-jun cDNAs were cloned, and their phylogenetic trees were constructed. The results demonstrate that the full-length c-fos cDNA is 1719 bp and contains a 1059-bp open reading frame (ORF) encoding 352 amino acids. The c-jun cDNA is 1931 bp and contains a 927-bp ORF encoding 308 amino acids. Moreover, constitutive expression of c-fos and c-jun was detected in all examined tissues of the silver carp using quantitative real-time polymerase chain reaction, and the liver showed the highest relative expression rate. In addition, we found that either 50 or 200 μg/kg crude microcystin exposure significantly promoted the transcription of c-fos, c-jun, and c-myc in the silver carp liver, suggesting that c-fos, c-jun, and c-myc may be involved in the hepatotoxicity of microcystins.
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, Henan, 453007, People's Republic of China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Li
authors.forename: Junguo
authors.initials: J
authors.lastname: Ma
authors.forename: Qian
authors.initials: Q
authors.lastname: Fang
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000378:metabolism*; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D052998:Microcystins / Q000633:toxicity*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism*; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics / Q000378:metabolism*
chemical_list: D029941:Fish Proteins; D052998:Microcystins; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun
keywords: Microcystins; Proto-Oncogene; Silver Carp; Transcription; cDNA Cloning