title: Expression and solubility testing in a high-throughput environment.
abstract: The expression and screening of the solubility of recombinant proteins is an important step in the high-throughput (HT) production of target proteins. For many applications, E. coli remains the most widely used expression system due to the relative ease of adapting it to HT pipelines. Herein is described a platform using a 96-well format for efficient expression and solubility screening of target proteins.
authors.affiliation: J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD, 20850, USA, kkwon@jcvi.org.
authors.forename: Keehwan
authors.initials: K
authors.lastname: Kwon
authors.forename: Scott N
authors.initials: SN
authors.lastname: Peterson
mesh_terms: D005786:Gene Expression Regulation; D057166:High-Throughput Screening Assays / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D012995:Solubility; D040681:Structural Homology, Protein
chemical_list: D011994:Recombinant Proteins