title: The relation of maternal serum eNOS, NOSTRIN and ADMA levels with aetiopathogenesis of preeclampsia and/or intrauterine fetal growth restriction.
abstract: OBJECTIVE
  The aim of present study was to assess the maternal serum endothelial nitric oxide synthase (eNOS), NOSTRIN (eNOS-trafficking inducer) and asymmetric dimethylarginine (ADMA) levels in pregnancies with intrauterine growth restriction (IUGR) in the presence or absence of preeclampsia and to compare the results with preeclamptic pregnant women with appropriate-for-gestational-age weight infants.
  
  
  METHODS
  The study was performed on 65 normotensive pregnant women with isolated IUGR, 64 preeclamptic women with IUGR, 51 preeclamptic women with normal intrauterine fetal growth and 65 healthy normotensive pregnant women with singleton uncomplicated pregnancies. Severe preeclampsia was defined as blood pressure> 160/110 mmHg with proteinuria> 5 g in a 24-h urinary protein excretion. IUGR were classified when the weight of the fetus was below the 10th centiles with disturbed placental function and abnormal ultrasonographic examination. The diagnosis was confirmed by the infant's weight at birth. The maternal serum eNOS, NOSTRIN and ADMA concentrations were determined using a sandwich enzyme-linked immunosorbent assays.
  
  
  RESULTS
  There were no statistically significant differences in the eNOS and NOSTRIN levels between studied groups of women. Increased levels of ADMA in both preeclamptic groups and in women with pregnancies complicated by isolated IUGR were observed.
  
  
  CONCLUSIONS
  Our results allow the conclusion that impaired NO bioavailability in pregnancies complicated by severe preeclampsia and/or IUGR result not from a reduced level or activity of eNOS or from its disturbed intracellular transport, but from increased ADMA levels, an endogenous inhibitor of the enzyme eNOS.
authors.affiliation: Chair and Department of Obstetrics and Perinatology, Medical University of Lublin , Poland.
authors.forename: Marzena
authors.initials: M
authors.lastname: Laskowska
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Laskowska
authors.forename: Jan
authors.initials: J
authors.lastname: Oleszczuk
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000328:Adult; D001120:Arginine / Q000031:analogs & derivatives* / Q000097:blood; D016022:Case-Control Studies; D004268:DNA-Binding Proteins; D005260:Female; D005317:Fetal Growth Retardation / Q000097:blood / Q000209:etiology*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000097:blood*; D052250:Nitric Oxide Synthase Type III / Q000097:blood*; D011225:Pre-Eclampsia / Q000097:blood / Q000209:etiology*; D011247:Pregnancy; D055815:Young Adult
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; C470345:NOSTRIN protein, human; C018524:N,N-dimethylarginine; D001120:Arginine; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III
keywords: Asymmetric dimethylarginine; IUGR; NOSTRIN; eNOS; preeclampsia