title: Poly(l-lactide-co-2-(2-methoxyethoxy)ethyl methacrylate): a biodegradable polymer with protein resistance.
abstract: We have synthesized poly(l-lactide-co-2-(2-methoxyethoxy)ethyl methacrylate) (LA-co-MEO2MA) containing both degradable and protein resistant units via hybrid copolymerization with (1-tert-butyl-4,4,4-tris(dimethylamino)-2,2-bis[tris(dimethylamino)phophoranylidenamino]-2Λ5,Λ5-catenadi(phosphazene) (t-BuP4) as the catalyst. Nuclear magnetic resonance (NMR) and differential scanning calorimetry (DSC) show that LA-co-MEO2MA is a random copolymer. The studies of quartz crystal microbalance with dissipation (QCM-D) demonstrate that the copolymer enzymaticlly degrades much faster than poly(l-lactide) (PLA) homopolymer due to its lower crystallinity. We have also investigated the adsorption of bovine serum albumin (BSA), lysozyme or fibrinogen on a LA-co-MEO2MA surface in real time by use of QCM-D and surface plasmon resonance (SPR). Our studies reveal that the polymer is protein resistant depending on MEO2MA content. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay experiments demonstrate that the polymer has a low cytotoxicity.
authors.affiliation: Faculty of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, PR China.
authors.forename: Jinbao
authors.initials: J
authors.lastname: Xu
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale, Department of Chemical Physics, University of Science and Technology of China, Hefei 230026, PR China.
authors.forename: Xuelin
authors.initials: X
authors.lastname: Fan
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale, Department of Chemical Physics, University of Science and Technology of China, Hefei 230026, PR China.
authors.forename: Jinxian
authors.initials: J
authors.lastname: Yang
authors.affiliation: Faculty of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, PR China.
authors.forename: Chunfeng
authors.initials: C
authors.lastname: Ma
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale, Department of Chemical Physics, University of Science and Technology of China, Hefei 230026, PR China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Ye
authors.affiliation: Faculty of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, PR China; Hefei National Laboratory for Physical Sciences at Microscale, Department of Chemical Physics, University of Science and Technology of China, Hefei 230026, PR China. Electronic address: gzzhang@ustc.edu.cn.
authors.forename: Guangzhao
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D005340:Fibrinogen / Q000737:chemistry* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008689:Methacrylates / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D013329:Structure-Activity Relationship; D013499:Surface Properties
chemical_list: D000970:Antineoplastic Agents; D008689:Methacrylates; D011091:Polyesters; C581192:poly(2-(2-methoxyethoxy)ethyl methacrylate); D012710:Serum Albumin, Bovine; C033616:poly(lactide); D005340:Fibrinogen; D009113:Muramidase
keywords: Enzymatic degradation; Poly(l-lactide); Protein resistance; QCM-D; SPR