title: Imaging early endothelial inflammation following stroke by core shell silica superparamagnetic glyconanoparticles that target selectin.
abstract: Activation of the endothelium is a pivotal first step for leukocyte migration into the diseased brain. Consequently, imaging this activation process is highly desirable. We synthesized carbohydrate-functionalized magnetic nanoparticles that bind specifically to the endothelial transmembrane inflammatory proteins E and P selectin. Magnetic resonance imaging revealed that the targeted nanoparticles accumulated in the brain vasculature following acute administration into a clinically relevant animal model of stroke, though increases in selectin expression were observed in both brain hemispheres. Nonfunctionalized naked particles also appear to be a plausible agent to target the ischemic vasculature. The importance of these findings is discussed regarding the potential for translation into the clinic.
authors.affiliation: Department of Experimental Neurology, Center for Stroke Research Berlin (CSB), Charité University Medicine , Charitéplatz 1, 10117 Berlin, Germany.
authors.forename: Tracy D
authors.initials: TD
authors.lastname: Farr
authors.forename: Chian-Hui
authors.initials: CH
authors.lastname: Lai
authors.forename: Dan
authors.initials: D
authors.lastname: Grünstein
authors.forename: Guillermo
authors.initials: G
authors.lastname: Orts-Gil
authors.forename: Cheng-Chung
authors.initials: CC
authors.lastname: Wang
authors.forename: Philipp
authors.initials: P
authors.lastname: Boehm-Sturm
authors.forename: Peter H
authors.initials: PH
authors.lastname: Seeberger
authors.forename: Christoph
authors.initials: C
authors.lastname: Harms
mesh_terms: D000818:Animals; D001921:Brain / Q000276:immunology / Q000473:pathology; D019040:E-Selectin / Q000032:analysis*; D006001:Glycoconjugates / Q000737:chemistry; D007249:Inflammation / Q000150:complications / Q000276:immunology / Q000473:pathology*; D058185:Magnetite Nanoparticles / Q000737:chemistry; D051379:Mice; D019007:P-Selectin / Q000032:analysis*; D012822:Silicon Dioxide / Q000737:chemistry; D020521:Stroke / Q000150:complications / Q000276:immunology / Q000473:pathology*
chemical_list: D019040:E-Selectin; D006001:Glycoconjugates; D058185:Magnetite Nanoparticles; D019007:P-Selectin; D012822:Silicon Dioxide