title: Isolation and identification of feline herpesvirus type 1 from a South China tiger in China.
abstract: In 2012, an FHV-1-like virus was isolated from a tiger that presented with clinical signs of sialorrhea, sneezing and purulent rhinorrhea. Isolation was performed with the FK81 cell line, and the virus was identified by PCR, transmission electron microscopy (TEM), and the phylogenetic analysis of the partial thymidine kinase (TK) and glycoprotein B (gB) genes. A total of 253 bp of the TK gene and 566 bp of the gB gene were amplified from the trachea of the tiger by PCR/RT-PCR method. Phylogenetic analysis showed that the isolate belonged to the same cluster with other FHV-1 strains obtained from GenBank. Herpes-like viruses with an envelope and diameters of approximately 200 nm were observed in the culture supernatants of FK81 cells inoculated with samples from the tiger. The FHV-1 infection was confirmed by an animal challenge experiment in a cat model. Our finding extends the host range of FHV-1 and has implications for FHV-1 infection and South China tiger conservation.
authors.affiliation: College of Wildlife Resource, Northeast Forestry University, Haerbin, 150040, China. xiaofengsht@163.com.
authors.forename: Heting
authors.initials: H
authors.lastname: Sun
authors.affiliation: College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, 730070, China. liyuanguo0520@163.com.
authors.forename: Yuanguo
authors.initials: Y
authors.lastname: Li
authors.affiliation: Wildlife Rescue and Breeding Centre of Jilin Province, Changchun, 130122, China. jiaoweiyi2013@163.com.
authors.forename: Weiyi
authors.initials: W
authors.lastname: Jiao
authors.affiliation: Wildlife Rescue and Breeding Centre of Jilin Province, Changchun, 130122, China. liucunfa2013@163.com.
authors.forename: Cunfa
authors.initials: C
authors.lastname: Liu
authors.affiliation: Wildlife Rescue and Breeding Centre of Jilin Province, Changchun, 130122, China. liuxiujuan2013@126.com.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Wildlife Rescue and Breeding Centre of Jilin Province, Changchun, 130122, China. haijunwang2013@163.com.
authors.forename: Haijun
authors.initials: H
authors.lastname: Wang
authors.affiliation: Shenzhen Wildlife Zoo, Shenzhen, 518055, China. huafuyou2013@163.com.
authors.forename: Fuyou
authors.initials: F
authors.lastname: Hua
authors.affiliation: Shenzhen Wildlife Zoo, Shenzhen, 518055, China. dongjianxiu2013@163.com.
authors.forename: Jianxiu
authors.initials: J
authors.lastname: Dong
authors.affiliation: Military Veterinary Research Institute of Academy of Military Medical Sciences, Changchun, 130122, China. fst0007@163.com.
authors.forename: Shengtao
authors.initials: S
authors.lastname: Fan
authors.affiliation: Military Veterinary Research Institute of Academy of Military Medical Sciences, Changchun, 130122, China. zhijun0215@gmail.com.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Military Veterinary Research Institute of Academy of Military Medical Sciences, Changchun, 130122, China. gaoyuwei@gmail.com.
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Military Veterinary Research Institute of Academy of Military Medical Sciences, Changchun, 130122, China. gywtext@hotmail.com.
authors.forename: Xianzhu
authors.initials: X
authors.lastname: Xia
mesh_terms: D000818:Animals; D002415:Cats; D002460:Cell Line; D002681:China; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D006566:Herpesviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D046010:Tigers; D014132:Trachea / Q000821:virology; D018140:Varicellovirus / Q000302:isolation & purification*; D014776:Virus Cultivation
chemical_list: D004279:DNA, Viral