title: Hypoxia-conditioned media allows species-specific attraction of bone marrow stromal cells without need for recombinant proteins.
abstract: BACKGROUND
  In vivo tissue regeneration depends on migration of stem cells into injured areas, their differentiation into specific cell types, and their interaction with other cells that are necessary to generate new tissue. Human mesenchymal stem cells, a subset of bone marrow stromal cells (BMSCs), can migrate and differentiate into osteoblasts in bone tissue. This can be facilitated by recombinant growth factors and cytokines. In many animal species, the availability of genomic sequences, recombinant proteins, and/or antibodies is limited so that new approaches are needed to generate resources that facilitate migration of stem cells into tissue defect areas. Here we used bone marrow stromal cells of human, ovine, equine, and canine origin to generate hypoxia-conditioned media (HCM) in order to attract BMSCs of the respective species in migration assays.
  
  
  RESULTS
  We show that HCM contain attractors even more potent than vascular endothelial growth factor and can therefore be used in many animal species without the need for purified proteins.
  
  
  CONCLUSIONS
  Generation of HCM is easy and cheap compared to preparation and purification of protein fractions and/or recombinant proteins. Hence, HCM could be applied in large animals (e.g. sheep, horse, dogs) for attraction of BMSCs into tissue defects caused by tumor resection or trauma.
authors.forename: Anastasia
authors.initials: A
authors.lastname: Gabrielyan
authors.forename: Sven
authors.initials: S
authors.lastname: Knaak
authors.forename: Michael
authors.initials: M
authors.lastname: Gelinsky
authors.forename: Stefan
authors.initials: S
authors.lastname: Arnhold
authors.affiliation: Department of Paediatrics, University Clinic Carl Gustav Carus, TU Dresden, Dresden, Germany. Angela.Roesen-Wolff@uniklinikum-dresden.de.
authors.forename: Angela
authors.initials: A
authors.lastname: Rösen-Wolff
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002465:Cell Movement; D017077:Culture Media, Conditioned; D004285:Dogs; D024243:HMGB1 Protein / Q000235:genetics / Q000378:metabolism; D006736:Horses; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D010100:Oxygen; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism; D012756:Sheep; D013045:Species Specificity; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D024243:HMGB1 Protein; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic; D042461:Vascular Endothelial Growth Factor A; D010100:Oxygen