title: Antioxidant mediated ameliorative steroidogenesis by Commelina benghalensis L. and Cissus quadrangularis L. against quinalphos induced male reproductive toxicity.
abstract: Quinalphos (QP) is speculated to cause endocrine disruption through the generation of reactive oxygen species (ROS) by oxidative stress (OS). Exposure of QP decreased testosterone level considerably which resulted in reduced viable sperms in mice. The QP induced toxicity is initiated by the formation of free radicals as it is evidenced from the increased Lipid peroxidation (LPO) and diminution of antioxidant enzymes in testicular tissue. Increased serum cholesterol and reduced testicular cholesterol indicated the inhibition of cholesterol transport and biosynthesis in testicular tissues. Lack of cholesterol in testicular tissue impaired the steroidogenesis by down-regulating the expression of StAR protein, Cytochrome P450, 3β-HSD and 17β-HSD leading to reduced testosterone level. Treatment of Commelina benganlensis (CBE) and Cissus quadrangularis (CQE) significantly recovered the alterations in antioxidant profiles as well as increased LPO, thereby recovering the decreased mRNA expression levels of intermediate enzymes. However, CQE effectively protected the OS and prevented the inhibition of steroidogenesis thereby preventing male infertility.
authors.affiliation: Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamilnadu, India.
authors.forename: Palanivel
authors.initials: P
authors.lastname: Kokilavani
authors.affiliation: Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamilnadu, India.
authors.forename: Udhayaraj
authors.initials: U
authors.lastname: Suriyakalaa
authors.affiliation: Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai 600 113, India.
authors.forename: Perumal
authors.initials: P
authors.lastname: Elumalai
authors.affiliation: Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamilnadu, India.
authors.forename: Bethunaicken
authors.initials: B
authors.lastname: Abirami
authors.affiliation: Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamilnadu, India.
authors.forename: Rajamanickam
authors.initials: R
authors.lastname: Ramachandran
authors.affiliation: Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamilnadu, India; Department of Bio Sciences and Technology, VIT University, Vellore 632 014, India.
authors.forename: Arunachalam
authors.initials: A
authors.lastname: Sankarganesh
authors.affiliation: Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamilnadu, India. Electronic address: achiramans@gmail.com.
authors.forename: Shanmugam
authors.initials: S
authors.lastname: Achiraman
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000235:genetics / Q000378:metabolism; D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002374:Catalase / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002784:Cholesterol / Q000097:blood / Q000378:metabolism; D032422:Cissus; D034822:Commelina; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D005979:Glutathione Peroxidase / Q000378:metabolism; D007306:Insecticides / Q000633:toxicity*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D051379:Mice; D009946:Organothiophosphorus Compounds / Q000633:toxicity*; D010750:Phosphoproteins / Q000235:genetics; D010936:Plant Extracts / Q000494:pharmacology*; D013076:Sperm Count; D013094:Spermatozoa / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000187:drug effects / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: D007306:Insecticides; D009946:Organothiophosphorus Compounds; D010750:Phosphoproteins; D010936:Plant Extracts; D000098682:Steroidogenic Acute Regulatory Protein; C009145:quinalphos; D013739:Testosterone; D007986:Luteinizing Hormone; D003577:Cytochrome P-450 Enzyme System; D002784:Cholesterol; D015067:17-Hydroxysteroid Dehydrogenases; D015096:3-Hydroxysteroid Dehydrogenases; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Antioxidants; C. benghalensis; C. quadrangularis; Oxidative stress; Quinalphos; Testosterone