title: Enhancement of diabetic wound repair using biodegradable nanofibrous metformin-eluting membranes: in vitro and in vivo.
abstract: This work developed biodegradable nanofibrous drug-eluting membranes that provided sustained release of metformin for repairing wounds associated with diabetes. To prepare the biodegradable membranes, poly-d-l-lactide-glycolide (PLGA) and metformin were first dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) and were spun into nanofibrous membranes by electrospinning. An elution method and an HPLC assay were utilized to characterize the in vivo and in vitro release rates of the pharmaceuticals from the membranes. The biodegradable nanofibrous membranes released high concentrations of metformin for more than three weeks. Moreover, nanofibrous metformin-eluting PLGA membranes were more hydrophilic and had a greater water-containing capacity than virgin PLGA fibers. The membranes also improved wound healing and re-epithelialization in diabetic rats relative to the control. The experimental results in this work suggest that nanofibrous metformin-eluting membranes were functionally active in the treatment of diabetic wounds and very effective as accelerators in the early stage of healing of such wounds.
authors.affiliation: Division of Cardiology, Department of Internal Medicine, Chang Gung Memorial Hospital-Linkou, Chang Gung University College of Medicine , Tao-Yuan, Taiwan.
authors.forename: Cheng-Hung
authors.initials: CH
authors.lastname: Lee
authors.forename: Ming-Jer
authors.initials: MJ
authors.lastname: Hsieh
authors.forename: Shang-Hung
authors.initials: SH
authors.lastname: Chang
authors.forename: Yu-Huang
authors.initials: YH
authors.lastname: Lin
authors.forename: Shih-Jung
authors.initials: SJ
authors.lastname: Liu
authors.forename: Tzu-Yu
authors.initials: TY
authors.lastname: Lin
authors.forename: Kuo-Chun
authors.initials: KC
authors.lastname: Hung
authors.forename: Jong-Hwei S
authors.initials: JH
authors.lastname: Pang
authors.forename: Jyuhn-Huarng
authors.initials: JH
authors.lastname: Juang
mesh_terms: D000818:Animals; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000493:pharmacokinetics*; D048909:Diabetes Complications / Q000188:drug therapy* / Q000503:physiopathology; D004337:Drug Carriers / Q000737:chemistry*; D005260:Female; D006801:Humans; D008297:Male; D008687:Metformin / Q000737:chemistry / Q000493:pharmacokinetics*; D057139:Nanofibers / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000188:drug therapy* / Q000503:physiopathology
chemical_list: D003692:Delayed-Action Preparations; D004337:Drug Carriers; D008687:Metformin