title: Artificial seawater culture of the gastropod Crepidula fornicata for studies of larval settlement and metamorphosis.
abstract: The slipper limpet, Crepidula fornicata, is a gastropod mollusc of growing importance as a research model in developmental biology and as an invasive organism. The large (>1 mm) veliger larvae of this species are well suited for neuroethological investigations of settlement and metamorphosis. In this chapter, methods are described for conditioning adult broodstock, growing microalgal food for larvae, and culturing larvae to metamorphic competence in artificial seawater. A protocol is also presented for obtaining electrophysiological recordings of ciliary arrest spikes from intact, behaving larvae, as putative neural correlates of larval settlement.
authors.affiliation: Department of Biology, Dickinson College, Carlisle, PA, USA.
authors.forename: Anthony
authors.initials: A
authors.lastname: Pires
mesh_terms: D000200:Action Potentials; D000818:Animals; D002923:Cilia / Q000502:physiology; D003470:Culture Media; D046508:Culture Techniques; D049851:Gastropoda / Q000166:cytology / Q000502:physiology*; D007814:Larva / Q000166:cytology / Q000502:physiology; D008675:Metamorphosis, Biological; D058086:Microalgae; D012098:Reproduction; D012623:Seawater
chemical_list: D003470:Culture Media