title: A reusable localized surface plasmon resonance biosensor for quantitative detection of serum squamous cell carcinoma antigen in cervical cancer patients based on silver nanoparticles array.
abstract: Squamous cell carcinoma antigen (SCCa), as a tumor biomarker, plays an important role in adjuvant diagnosis, treatment evaluation, and prognosis prediction for cervical cancer patients. Localized surface plasmon resonance (LSPR) technique based on noble metal nanoparticles bypasses the disadvantages of traditional testing strategies, in terms of free-labeling, short assay time, good sensitivity, and selectivity. Herein, we develop a novel and reusable LSPR biosensor for the detection of SCCa. First, a triangle-shaped silver nanoparticle array was fabricated using the nanosphere lithography method. Next, we investigated and verified the feasibility of amino coupling method using 11-mercaptoundecanoic acid (MUA) to form a functionalized chip surface with monoclonal anti-SCCa antibodies on the silver nanoparticles for distinct detection of SCCa. Different concentrations of SCCa were successfully tested in both buffer and human serum by the ultrasensitive and specific LSPR system, with a linear quantitative detection range of 0.1-1,000 pM under optimal conditions. With appropriate regeneration solution, for example 50 mM glycine-HCl (pH 2.0), the LSPR biosensor featured effective fabrication reproducibility, which reduced both production cost and testing time. Our study represents the first application of the LSPR biosensor in cervical cancer, and demonstrates that the rapid, simple, and reusable nanochip can serve as a potential alternative for clinical serological diagnosis of SCCa in cervical cancer patients.
authors.affiliation: Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, People's Republic of China.
authors.forename: Qianying
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, People's Republic of China.
authors.forename: Ruiqi
authors.initials: R
authors.lastname: Duan
authors.affiliation: Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, People's Republic of China.
authors.forename: Jialing
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Quan
authors.affiliation: State Key Laboratory of Optical Technologies for Microfabrication, Institute of Optics and Electronics, Chinese Academy of Science, Chengdu, Sichuan, People's Republic of China.
authors.forename: Huan
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, People's Republic of China.
authors.forename: Mingrong
authors.initials: M
authors.lastname: Xi
mesh_terms: D000951:Antigens, Neoplasm / Q000097:blood*; D014408:Biomarkers, Tumor / Q000097:blood*; D002294:Carcinoma, Squamous Cell / Q000097:blood*; D016022:Case-Control Studies; D004867:Equipment Design; D005260:Female; D006801:Humans; D056656:Lab-On-A-Chip Devices / Q000706:statistics & numerical data; D053768:Metal Nanoparticles; D050997:Nanomedicine; D015203:Reproducibility of Results; D015843:Serpins / Q000097:blood*; D012834:Silver; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D002583:Uterine Cervical Neoplasms / Q000097:blood*
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D015843:Serpins; C051072:squamous cell carcinoma-related antigen; D012834:Silver
keywords: biosensor; cervical cancer biomarker; localized surface plasmon resonance; nanotechnology; squamous cell carcinoma antigen