title: Effects of konjac glucomannan, inulin and cellulose on acute colonic responses to genotoxic azoxymethane.
abstract: Mice were fed low-fibre, or that supplemented with soluble fibre (konjac glucomannan, KGM; inulin), or insoluble fibre (cellulose) to determine how these three fibres modulated the acute colonic responses to an azoxymethane (AOM) treatment. Results indicated that KGM and inulin exerted greater anti-genotoxic effects compared to cellulose and up-regulated the gene expressions of glutathione S-transferase and antioxidant enzymes. The apoptotic index in the distal colon was the greatest and the expression of Bcl-2 was the lowest in the KGM group 24h after the AOM treatment. On the other hand, the proliferative index and expression of Cyclin D1 were lower in all fibre groups. Furthermore, KGM increased cecal short-chain fatty acid contents, and both KGM and inulin increased fecal probiotic concentrations. This study suggested that soluble fibres were more effective than cellulose on ameliorating AOM-induced genotoxicity by up-regulating antioxidant enzyme genes, and enhancing epithelium apoptosis by down-regulating Bcl-2.
authors.affiliation: School of Nutrition, Chung Shan Medical University, No. 110, Sec. 1 Jianguo N. Rd., Taichung City 402, Taiwan.
authors.forename: Wen-Tzu
authors.initials: WT
authors.lastname: Wu
authors.affiliation: Department of Pathology, Chung Shan Medical University Hospital, No. 110, Sec. 1 Jianguo N. Rd., Taichung City 402, Taiwan.
authors.forename: Lien-Chuan
authors.initials: LC
authors.lastname: Yang
authors.affiliation: School of Nutrition, Chung Shan Medical University, No. 110, Sec. 1 Jianguo N. Rd., Taichung City 402, Taiwan; Department of Nutrition, Chung Shan Medical University Hospital, Taichung, Taiwan. Electronic address: hlchen908@gmail.com.
authors.forename: Hsiao-Ling
authors.initials: HL
authors.lastname: Chen
mesh_terms: D031056:Amorphophallus / Q000737:chemistry*; D000818:Animals; D001397:Azoxymethane / Q000633:toxicity*; D002482:Cellulose / Q000378:metabolism*; D003106:Colon / Q000187:drug effects* / Q000378:metabolism / Q000382:microbiology; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000178:diet therapy* / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects; D005243:Feces / Q000382:microbiology; D006801:Humans; D007444:Inulin / Q000378:metabolism*; D008297:Male; D008351:Mannans / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D064307:Microbiota; D009153:Mutagens / Q000633:toxicity; D010936:Plant Extracts / Q000378:metabolism*
chemical_list: D008351:Mannans; D009153:Mutagens; D010936:Plant Extracts; C022901:(1-6)-alpha-glucomannan; D002482:Cellulose; D007444:Inulin; D001397:Azoxymethane
keywords: Apoptosis; Azoxymethane; DNA damage; Inulin; Konjac glucomannan; Proliferation