title: Usefulness of pan-fungal NASBA test for surveillance of environmental fungal contamination in a protected hematology unit.
abstract: A pan-fungal nucleic acid sequence based applification (NASBA) test was adapted and used for the first time to detect and quantify the level of filamentous fungi in environmental samples. Surface samples (n = 356) collected in a controlled air flow hematology ward were tested by mycological culture and the pan-fungal NASBA test. The overall percentage of agreement between culture and NASBA was 88%, the Kappa coefficient was equal to 0.61 (95%CI = [0.51; 0.72]). This pan-fungal NASBA test could be a promising tool to rapidly monitor the absence of molds in controlled environments.
authors.affiliation: Laboratory of Parasitology and Mycology.
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Brenier-Pinchart
authors.forename: Hafid
authors.initials: H
authors.lastname: Abaibou
authors.forename: Thomas
authors.initials: T
authors.lastname: Berendsen
authors.forename: Gautier
authors.initials: G
authors.lastname: Szymanski
authors.forename: Messalia
authors.initials: M
authors.lastname: Beghri
authors.forename: Sébatien
authors.initials: S
authors.lastname: Bailly
authors.forename: Frédérick
authors.initials: F
authors.lastname: Lasnet
authors.forename: Anne
authors.initials: A
authors.lastname: Thiebaut-Bertrand
authors.forename: Claude
authors.initials: C
authors.lastname: Mabilat
authors.forename: Hervé
authors.initials: H
authors.lastname: Pelloux
mesh_terms: D000818:Animals; D004783:Environmental Microbiology; D062665:Epidemiological Monitoring; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006748:Hospital Departments; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D021161:Self-Sustained Sequence Replication / Q000379:methods*
keywords: NASBA; hematology unit; invasive aspergillosis; molds; protected environments