title: A novel platelet concentrate: titanium-prepared platelet-rich fibrin.
abstract: We developed a new product called titanium-prepared platelet-rich fibrin (T-PRF). The T-PRF method is based on the hypothesis that titanium may be more effective in activating platelets than the silica activators used with glass tubes in Chouckroun's leukocyte- and platelet-rich fibrin (L-PRF) method. In this study, we aimed to define the structural characteristics of T-PRF and compare it with L-PRF. Blood samples were collected from 10 healthy male volunteers. The blood samples were drawn using a syringe. Nine milliliters was transferred to a dry glass tube, and 9 mL was transferred to a titanium tube. Half of each clot (i.e., the blood that was clotted using T-PRF or L-PRF) was processed with a scanning electron microscope (SEM). The other half of each clot was processed for fluorescence microscopy analysis and light microscopy analysis. The T-PRF samples seemed to have a highly organized network with continuous integrity compared to the other L-PRF samples. Histomorphometric analysis showed that T-PRF fibrin network covers larger area than L-PRF fibrin network; also fibrin seemed thicker in the T-PRF samples. This is the first human study to define T-PRF as an autogenous leukocyte- and platelet-rich fibrin product. The platelet activation by titanium seems to offer some high characteristics to T-PRF.
authors.affiliation: Department of Periodontology, Haydarpasa Training Hospital, Gulhane Military Medical Academy, Üsküdar, 34618 Istanbul, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Tunalı
authors.affiliation: Department of Periodontology, School of Dentistry, Cumhuriyet University, 58140 Sivas, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Özdemir
authors.affiliation: Department of Medical Pathology, Gulhane Military Medical Academy, 34618 Istanbul, Turkey.
authors.forename: Zafer
authors.initials: Z
authors.lastname: Küçükodacı
authors.affiliation: Department of Prosthodontics, School of Dentistry, Selcuk University, 42250 Konya, Turkey.
authors.forename: Serhan
authors.initials: S
authors.lastname: Akman
authors.affiliation: Department of Periodontology, School of Dentistry, Kocaeli University, 41380 Izmit, Turkey.
authors.forename: Emre
authors.initials: E
authors.lastname: Yaprak
authors.affiliation: Department of Periodontology, School of Dentistry, Cumhuriyet University, 58140 Sivas, Turkey.
authors.forename: Hülya
authors.initials: H
authors.lastname: Toker
authors.affiliation: Department of Periodontology, School of Dentistry, Istanbul University, 34303 Istanbul, Turkey.
authors.forename: Erhan
authors.initials: E
authors.lastname: Fıratlı
mesh_terms: D000328:Adult; D001777:Blood Coagulation / Q000187:drug effects; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D005337:Fibrin / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D014025:Titanium / Q000494:pharmacology*; D055815:Young Adult
chemical_list: D005337:Fibrin; D014025:Titanium