title: Producing and concentrating lenti-Cre for mouse infections.
abstract: Lentiviral vectors offer versatility as vehicles for gene delivery. They can transduce a wide range of cell types and integrate into the host genome, which results in long-term expression of the transgene (Cre) both in vitro and in vivo. This protocol describes how lentiviral particles are produced, purified, and concentrated.
authors.affiliation: Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Department of Pathology, Harvard Medical School, Charlestown, Massachusetts 02129;
authors.forename: Jessica J
authors.initials: JJ
authors.lastname: Gierut
authors.forename: Tyler E
authors.initials: TE
authors.lastname: Jacks
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Haigis
mesh_terms: D000818:Animals; D005822:Genetic Vectors; D007239:Infections / Q000628:therapy*; D019426:Integrases / Q000235:genetics*; D016086:Lentivirus / Q000235:genetics*; D051379:Mice
chemical_list: C045073:Cre recombinase; D019426:Integrases