title: Role of epidermal stem cells in repair of partial-thickness burn injury after using Moist Exposed Burn Ointment (MEBO(®)) histological and immunohistochemical study.
abstract: Moist Exposed Burn Ointment (MEBO(®)) is widely used topical agent applied on skin burn. This study investigated the effect of MEBO topical application on activation and proliferation of epidermal stem cells through the immunohistochemical localization of cytokeratin 19 (CK19) as a known marker expressed in epidermal stem cells. Biopsies from normal skin and burn wounds were taken from 21 patients with partial thickness burn 1, 4, 7, 14, 21, and 28 days after treatment with MEBO. Tissue sections were prepared for histological study and for CK19 immunohistochemical localization. In control skin, only few cells showed a positive CK19 immune-reaction. Burned skin showed necrosis of full thickness epidermis that extended to dermis. Gradual regeneration of skin accompanied with an enhancement in CK19 immune-reactivity was noted 4, 7, 14 and 21 days after treatment with MEBO. On day 28, a complete regeneration of skin was observed with a return of CK19 immune-reactivity to the basal pattern again. In conclusion, the enhancement of epidermal stem cell marker CK19 after treatment of partial thickness burn injuries with MEBO suggested the role of MEBO in promoting epidermal stem cell activation and proliferation during burn wound healing.
authors.affiliation: Plastic and Reconstructive Surgery Department, Faculty of Medicine, Mansoura University, Egypt.
authors.forename: M R
authors.initials: MR
authors.lastname: El-Hadidy
authors.affiliation: Histology & Cell Biology Department, Faculty of Medicine, Mansoura University, Egypt.
authors.forename: A R
authors.initials: AR
authors.lastname: El-Hadidy
authors.affiliation: Plastic and Reconstructive Surgery Department, Faculty of Medicine, Mansoura University, Egypt.
authors.forename: A
authors.initials: A
authors.lastname: Bhaa
authors.affiliation: Histology & Cell Biology Department, Faculty of Medicine, Mansoura University, Egypt. Electronic address: sanaasker@yahoo.com.
authors.forename: S A
authors.initials: SA
authors.lastname: Asker
authors.affiliation: Histology & Cell Biology Department, Faculty of Medicine, Mansoura University, Egypt.
authors.forename: S A
authors.initials: SA
authors.lastname: Mazroa
mesh_terms: D000293:Adolescent; D000328:Adult; D018941:Antigens, CD19 / Q000378:metabolism; D002056:Burns / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D020405:Dermis / Q000378:metabolism / Q000473:pathology; D004817:Epidermis / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D012855:Sitosterols / Q000008:administration & dosage*; D013234:Stem Cells / Q000378:metabolism / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D018941:Antigens, CD19; D012855:Sitosterols; C472006:moist exposed burn ointment
keywords: Burn; Cytokeratin 19; Epidermal stem cells; Immunohistochemistry; MEBO