title: Effect of allyl isothiocyanate on ultra-structure and the activities of four enzymes in adult Sitophilus zeamais.
abstract: Rarefaction and vacuolization of the mitochondrial matrix of AITC-treated (allyl isothiocyanate-treated) adult Sitophilus zeamais were evident according to the ultra-structural by TEM. Four important enzymes in adult S. zeamais were further studied after fumigation treatment with allyl isothiocyanate (AITC) extracted from Armoracia rusticana roots and shoots. The enzymes were glutathione S-transferase (GST), catalase (CAT), cytochrome c oxidase, and acetylcholinesterase (AChE). The results indicated that the activities of the four enzymes were strongly time and dose depended. With prolonged exposure time, treatment with 0.74μg/mL AITC inhibited the activities of cytochrome c oxidase, AChE, and CAT, but induced the activity of GST. The activities of cytochrome c oxidase, AChE, and CAT were remarkably induced at a low AITC dosage (0.25μg/mL), but were restrained with increased AITC dosage. The activity of GST was inhibited at a low AITC dosage (0.5μg/mL), but was induced at a high AITC dosage (1.5μg/mL). According to the results of TEM, toxic symptoms and enzymes activities, it suggested that mitochondrial maybe the one site of action of AITC against the adult S. zeamais and it also suggested that cytochrome c oxidase maybe one target protein of AITC against the adult S. zeamais, which need to further confirmed by protein function tested.
authors.affiliation: Research & Development Center of Biorational Pesticide, Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Northwest A&F University, Yangling 712100, China; Shaanxi Research Center of Biopesticide Engineering & Technology, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: huawu686@aliyun.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Wu
authors.affiliation: Research & Development Center of Biorational Pesticide, Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Northwest A&F University, Yangling 712100, China.
authors.forename: Xue-ru
authors.initials: XR
authors.lastname: Liu
authors.affiliation: Research & Development Center of Biorational Pesticide, Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Northwest A&F University, Yangling 712100, China.
authors.forename: Dong-dong
authors.initials: DD
authors.lastname: Yu
authors.affiliation: Research & Development Center of Biorational Pesticide, Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Northwest A&F University, Yangling 712100, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Research & Development Center of Biorational Pesticide, Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Northwest A&F University, Yangling 712100, China; Shaanxi Research Center of Biopesticide Engineering & Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Jun-tao
authors.initials: JT
authors.lastname: Feng
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D031215:Armoracia / Q000737:chemistry; D002374:Catalase / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D005520:Food Preservatives / Q000633:toxicity*; D005651:Fumigation; D005982:Glutathione Transferase / Q000378:metabolism; D007306:Insecticides / Q000633:toxicity*; D017879:Isothiocyanates / Q000633:toxicity*; D046529:Microscopy, Electron, Transmission; D048889:Weevils / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure
chemical_list: D005520:Food Preservatives; D007306:Insecticides; D017879:Isothiocyanates; C004471:allyl isothiocyanate; D002374:Catalase; D003576:Electron Transport Complex IV; D005982:Glutathione Transferase; D000110:Acetylcholinesterase
keywords: AITC; Catalase; Cytochrome c oxidase; Glutathione S-transferase; Sitophilus zeamais; Ultra-structural