title: Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering.
abstract: Sensitive and specific visualization of small biomolecules in living systems is highly challenging. We report stimulated Raman-scattering imaging of alkyne tags as a general strategy for studying a broad spectrum of small biomolecules in live cells and animals. We demonstrate this technique by tracking alkyne-bearing drugs in mouse tissues and visualizing de novo synthesis of DNA, RNA, proteins, phospholipids and triglycerides through metabolic incorporation of alkyne-tagged small precursors.
authors.affiliation: Department of Chemistry, Columbia University, New York, New York, USA.
authors.forename: Lu
authors.initials: L
authors.lastname: Wei
authors.affiliation: Department of Chemistry, Columbia University, New York, New York, USA.
authors.forename: Fanghao
authors.initials: F
authors.lastname: Hu
authors.affiliation: Department of Chemistry, Columbia University, New York, New York, USA.
authors.forename: Yihui
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Chemistry, Columbia University, New York, New York, USA.
authors.forename: Zhixing
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA.
authors.forename: Chih-Chun
authors.initials: CC
authors.lastname: Lin
authors.affiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA.
authors.forename: Meng C
authors.initials: MC
authors.lastname: Wang
authors.affiliation: 1] Department of Chemistry, Columbia University, New York, New York, USA. [2] Kavli Institute for Brain Science, Columbia University, New York, New York, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Min
mesh_terms: D000480:Alkynes; D000818:Animals; D004247:DNA / Q000096:biosynthesis; D005786:Gene Expression Regulation / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D015394:Molecular Structure; D009281:Naphthalenes; D011506:Proteins / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis; D013059:Spectrum Analysis, Raman / Q000379:methods*; D000077291:Terbinafine
chemical_list: D000480:Alkynes; D009281:Naphthalenes; D011506:Proteins; D012313:RNA; D004247:DNA; D000077291:Terbinafine