title: Performance of panfungal--and specific-PCR-based procedures for etiological diagnosis of invasive fungal diseases on tissue biopsy specimens with proven infection: a 7-year retrospective analysis from a reference laboratory.
abstract: A retrospective analysis of real-time PCR (RT-PCR) results for 151 biopsy samples obtained from 132 patients with proven invasive fungal diseases was performed. PCR-based techniques proved to be fast and sensitive and enabled definitive diagnosis in all cases studied, with detection of a total of 28 fungal species.
authors.affiliation: Mycology Department, National Centre for Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Buitrago
authors.forename: L
authors.initials: L
authors.lastname: Bernal-Martinez
authors.forename: M V
authors.initials: MV
authors.lastname: Castelli
authors.forename: J L
authors.initials: JL
authors.lastname: Rodriguez-Tudela
authors.forename: M
authors.initials: M
authors.lastname: Cuenca-Estrella
mesh_terms: D001706:Biopsy / Q000379:methods; D004271:DNA, Fungal / Q000235:genetics; D005658:Fungi / Q000235:genetics*; D006801:Humans; D009181:Mycoses / Q000175:diagnosis* / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012189:Retrospective Studies
chemical_list: D004271:DNA, Fungal