title: Development of a two-step multiplex PCR assay for typing of capsular polysaccharide synthesis gene clusters of Streptococcus suis.
abstract: We developed a practical and easy two-step multiplex PCR assay to aid in serotyping of Streptococcus suis. The assay accurately typed almost all of the serotype reference strains and field isolates of various serotypes and also identified the genotypes of capsular polysaccharide synthesis gene clusters of some serologically nontypeable strains.
authors.affiliation: Bacterial and Parasitic Diseases Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Okura
authors.forename: Claude
authors.initials: C
authors.lastname: Lachance
authors.forename: Makoto
authors.initials: M
authors.lastname: Osaki
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Sekizaki
authors.forename: Fumito
authors.initials: F
authors.lastname: Maruyama
authors.forename: Takashi
authors.initials: T
authors.lastname: Nozawa
authors.forename: Ichiro
authors.initials: I
authors.lastname: Nakagawa
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Hamada
authors.forename: Céline
authors.initials: C
authors.lastname: Rossignol
authors.forename: Marcelo
authors.initials: M
authors.lastname: Gottschalk
authors.forename: Daisuke
authors.initials: D
authors.lastname: Takamatsu
mesh_terms: D000818:Animals; D016667:Bacterial Capsules / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D011134:Polysaccharides / Q000235:genetics*; D012703:Serotyping / Q000379:methods; D013290:Streptococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D017011:Streptococcus suis / Q000235:genetics*; D013552:Swine / Q000382:microbiology; D013553:Swine Diseases / Q000175:diagnosis / Q000382:microbiology
chemical_list: D011134:Polysaccharides