title: Isolation and biosynthesis of preussin B, a pyrrolidine alkaloid from Simplicillium lanosoniveum.
abstract: A new pyrrolidine alkaloid, preussin B (1), was isolated from the culture extract of the fungus Simplicillium lanosoniveum TAMA 173 along with the known congener preussin (2). The structure and absolute configuration of 1 were determined by spectroscopic analysis and spectral comparison with 2. Feeding experiments with 13C-labeled precursors revealed that the pyrrolidine ring of 1 was assembled from acetate and l-phenylalanine by a PKS-NRPS hybrid biosynthetic pathway.
authors.affiliation: Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University , 5180 Kurokawa, Imizu, Toyama 939-0398, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Fukuda
authors.forename: Yuri
authors.initials: Y
authors.lastname: Sudoh
authors.forename: Yuki
authors.initials: Y
authors.lastname: Tsuchiya
authors.forename: Toru
authors.initials: T
authors.lastname: Okuda
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Igarashi
mesh_terms: D000470:Alkaloids / Q000096:biosynthesis / Q000378:metabolism; D000841:Anisomycin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D006999:Hypocreales / Q000737:chemistry*; D015394:Molecular Structure; D009097:Multienzyme Complexes / Q000096:biosynthesis / Q000378:metabolism; D010453:Peptide Synthases / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D010649:Phenylalanine / Q000096:biosynthesis / Q000378:metabolism; D013237:Stereoisomerism; D013302:Streptomyces / Q000378:metabolism
chemical_list: D000470:Alkaloids; D009097:Multienzyme Complexes; C000589754:preussin B; C058280:preussin; D010649:Phenylalanine; D000841:Anisomycin; D010453:Peptide Synthases