title: Three types of macrophagic cells in the mesentery of mice with special reference to LYVE-1-immunoreactive cells.
abstract: Immunohistochemistry using whole mount preparations of the murine mesentery revealed two types of LYVE-1-immunoreactive cells with dendritic morphology other than F4/80(+) typical macrophages.The two types of LYVE-1(+) cells were regularly distributed with constant intervals throughout the mesentery and appeared to possess their own territory. Both types of LYVE-1(+) cells were weakly or moderately immunopositive for F4/80 antibody, a marker of macrophages,while F4/80(+) round macrophages were absolutely free from the LYVE-1 immunoreactivity. Only macrophages could ingest latex particles of 20 nm in diameter 3 h after a peritoneal injection.Peritoneal administration of lipopolysaccharide (LPS) induced a rapid reduction of LYVE-1 immunoreactivity in the cells with dendritic morphology followed by an increased immunoreactivity to F4/80 antibody, and simultaneously by dynamic changes in their shape. Under normal conditions,F4/80(+) macrophages in various connective tissues expressed LYVE-1, in contrast to lack of LYVE-1 in F4/80(+) macrophages within the parenchyma of visceral organs and macrophages residing in hepatic sinusoids and pulmonary alveoli. LYVE-1 may play a role in cell adhesion and migration of macrophagic cells within connective tissues rich in hyaluronan, and loss of LYVE-1 becomes a reliable sign of activated conditions in inflammation.
authors.affiliation: Laboratory of Histology and Cytology, Graduate School of Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Miao
authors.initials: M
authors.lastname: Zheng
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Kimura
authors.forename: Junko
authors.initials: J
authors.lastname: Nio-Kobayashi
authors.forename: Hiromi
authors.initials: H
authors.lastname: Takahashi-Iwanaga
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Iwanaga
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000276:immunology / Q000378:metabolism; D005260:Female; D006023:Glycoproteins / Q000276:immunology* / Q000378:metabolism*; D007150:Immunohistochemistry; D007249:Inflammation / Q000276:immunology / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism* / Q000648:ultrastructure; D026901:Membrane Transport Proteins; D008643:Mesentery / Q000166:cytology* / Q000276:immunology*; D051379:Mice
chemical_list: D000943:Antigens, Differentiation; D006023:Glycoproteins; D008070:Lipopolysaccharides; D026901:Membrane Transport Proteins; C492273:LYVE1 protein, mouse; C036763:monocyte-macrophage differentiation antigen