title: Analysis of differential gene expression and novel transcript units of ovine muscle transcriptomes.
abstract: In this study, we characterized differentially expressed genes (DEGs) between the muscle transcriptomes of Small-tailed Han sheep and Dorper sheep and predicted novel transcript units using high-throughput RNA sequencing technology. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that 1,300 DEGs were involved in cellular processes, metabolic pathways, and the actin cytoskeleton pathway. Importantly, we identified 34 DEGs related to muscle cell development and differentiation. Additionally, we were able to optimize the gene structure and predict the untranslated regions (UTRs) for some of the DEGs. Among the 123,678 novel predicted transcript units (TUs), 15,015 units were predicted protein sequences. The reliability of the sequencing data was verified through qRT-PCR analysis of 12 genes. These results will provide useful information for functional genetic research in the future.
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian, China ; College of Biological and Agricultural Engineering, Weifang University, Key Laboratory of Biochemistry and Molecular Biology in Universities of Shandong, Weifang, China.
authors.forename: Chunlan
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian, China.
authors.forename: Guizhi
authors.initials: G
authors.lastname: Wang
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian, China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian, China.
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Ji
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Dong
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian, China.
authors.forename: Tianle
authors.initials: T
authors.lastname: Chao
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D017931:DNA Primers / Q000235:genetics; D020869:Gene Expression Profiling / Q000662:veterinary; D063990:Gene Ontology; D059014:High-Throughput Nucleotide Sequencing; D032342:Muscle Cells / Q000502:physiology; D018482:Muscle, Skeletal / Q000378:metabolism*; D060066:Protein Interaction Maps; D060888:Real-Time Polymerase Chain Reaction; D012756:Sheep / Q000235:genetics* / Q000378:metabolism*; D013045:Species Specificity; D059467:Transcriptome / Q000235:genetics*; D020506:Untranslated Regions / Q000235:genetics
chemical_list: D017931:DNA Primers; D020506:Untranslated Regions