title: Detection of drug resistant Mycobacterium tuberculosis among patients with and without HIV infection in a rural setting.
abstract: OBJECTIVE
  To analyse the sensitivity of Mycobacterium tuberculosis by nitrate reductase assay (NRA) and the Hain molecular line probe assay (LPA) in sputa of tuberculosis (TB)/HIV co-infected patients in Guyana.
  
  
  METHODS
  Sputum samples were collected from known TB patients at Georgetown Chest Clinic and were analysed at the Reference Laboratory, Guyana, over the period April 2010 to April 2011.
  
  
  RESULTS
  Both methods recorded greater sensitivity for rifampin (RIF) than of isoniazid (INH). Both methods detected four RIF resistant, two INH resistant and two multi-drug resistant (MDR) strains and they had greater negative agreement indices than positive agreement indices.
  
  
  CONCLUSIONS
  It was established that the sensitivity of Mycobacterium tuberculosis by the NRA and Hain LPA in TB/HIV co-infected patients has acceptable correlation and that HIV infection does not affect drug susceptibility testing.
authors.affiliation: Faculty of Health Sciences, University of Guyana, Guyana, South America. kuruprajini@yahoo.com
authors.forename: R
authors.initials: R
authors.lastname: Kurup
authors.affiliation: Faculty of Health Sciences, University of Guyana, Guyana, South America.
authors.forename: C
authors.initials: C
authors.lastname: George
mesh_terms: D000995:Antitubercular Agents / Q000627:therapeutic use; D016022:Case-Control Studies; D060085:Coinfection / Q000150:complications / Q000175:diagnosis; D024881:Drug Resistance, Bacterial; D024901:Drug Resistance, Multiple, Bacterial; D006172:Guyana; D015658:HIV Infections / Q000150:complications; D006801:Humans; D007538:Isoniazid / Q000627:therapeutic use; D008826:Microbial Sensitivity Tests / Q000379:methods*; D015336:Molecular Probe Techniques; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D009566:Nitrates; D012293:Rifampin / Q000627:therapeutic use; D012424:Rural Population; D013183:Sputum / Q000382:microbiology*; D018088:Tuberculosis, Multidrug-Resistant / Q000150:complications / Q000175:diagnosis*; D014397:Tuberculosis, Pulmonary / Q000150:complications / Q000175:diagnosis*
chemical_list: D000995:Antitubercular Agents; D009566:Nitrates; D007538:Isoniazid; D012293:Rifampin