title: Human papillomavirus detection and genotyping, by HC2, full-spectrum HPV and molecular beacon real-time HPV assay in an Irish colposcopy clinic.
abstract: Cervical screening programmes are moving towards HPV testing as part of the screening process and as a triage for colposcopy. Three HPV detection methods were evaluated using cervical cytology specimens from colposcopy patients. PreservCyt™ liquid based cytology specimens from 241 women attending colposcopy clinics with greater than 2 persistently abnormal smears were recruited through the Coombe Women and Infants University Hospital, Dublin. HPV DNA was detected by Hybrid Capture (HC2) for 13 high-risk HPV types, Full-Spectrum HPV (FS-HPV) for 49 high and low-risk types and Molecular Beacon Real-Time HPV assay (MBRT-HPV) for 16 high and low-risk types. HPV genotyping was performed using Linear Array HPV Assay (LA-HPV). HPV was detected in 83.3% (195/234), 91.9% (217/236) and 80.1% (169/211) of cytology specimens by HC2, FS-HPV and MBRT-HPV, HPV DNA detection assays. The sensitivity of the assays for the detection of high-risk HPV in cytology specimens that had a Cervical Intraepithelial Neoplasia Grade 2+ result by histology were, 98%, 97% and 94% for HC2, FS-HPV and MBRT-HPV assays with positive predictive values of 94.1%, 94.1% and 97.3%. The most common HPV genotypes were HPV 16, 31, 33, 58, 42, 61 and 53, and the most common high-risk HPV genotypes were HPV 16, 31, 33, 58, 18, 45, 59, 51, 56 and 39, with detection of multiple infections in 57.7% of all cases. FS-HPV and MBRT-HPV are highly sensitive and have a similarly high PPV as the HC2 assay for detection of HPV in patients with Cervical Intraepithelial Neoplasia Grade 2+ disease. HPV genotyping of women with persistent abnormalities is warranted prior to the introduction of HPV DNA testing in a colposcopy setting.
authors.affiliation: Department of Histopathology, School of Medicine, Trinity College Dublin, Dublin 2, Ireland; Molecular Pathology Laboratory, Department of Pathology, Coombe Women and Infants University Hospital, Dublin 8, Ireland. Electronic address: keeganhe@tcd.ie.
authors.forename: Helen
authors.initials: H
authors.lastname: Keegan
authors.affiliation: Molecular Pathology Laboratory, Department of Pathology, Coombe Women and Infants University Hospital, Dublin 8, Ireland.
authors.forename: Loretto
authors.initials: L
authors.lastname: Pilkington
authors.affiliation: Department of Histopathology, School of Medicine, Trinity College Dublin, Dublin 2, Ireland; Molecular Pathology Laboratory, Department of Pathology, Coombe Women and Infants University Hospital, Dublin 8, Ireland.
authors.forename: Jamie
authors.initials: J
authors.lastname: McInerney
authors.affiliation: GenoID Ltd., 1399, Budapest Pf 672, Hungary.
authors.forename: Csaba
authors.initials: C
authors.lastname: Jeney
authors.affiliation: GenoID Ltd., 1399, Budapest Pf 672, Hungary.
authors.forename: Márta
authors.initials: M
authors.lastname: Benczik
authors.affiliation: Department of Obstetrics and Gynaecology, Coombe Women and Infants University Hospital, Dublin 8, Ireland.
authors.forename: Sinead
authors.initials: S
authors.lastname: Cleary
authors.affiliation: Department of Obstetrics and Gynaecology, Coombe Women and Infants University Hospital, Dublin 8, Ireland.
authors.forename: Gunther
authors.initials: G
authors.lastname: von Bunau
authors.affiliation: Department of Obstetrics and Gynaecology, Coombe Women and Infants University Hospital, Dublin 8, Ireland.
authors.forename: Michael
authors.initials: M
authors.lastname: Turner
authors.affiliation: Department of Obstetrics and Gynaecology, Coombe Women and Infants University Hospital, Dublin 8, Ireland.
authors.forename: Tom
authors.initials: T
authors.lastname: D'Arcy
authors.affiliation: Department of Histopathology, School of Medicine, Trinity College Dublin, Dublin 2, Ireland.
authors.forename: Sharon
authors.initials: S
authors.lastname: O' Toole
authors.affiliation: GenoID Ltd., 1399, Budapest Pf 672, Hungary.
authors.forename: Borbála
authors.initials: B
authors.lastname: Pal-Szenthe
authors.affiliation: GenoID Ltd., 1399, Budapest Pf 672, Hungary.
authors.forename: Borbàla
authors.initials: B
authors.lastname: Kaltenecker
authors.affiliation: GenoID Ltd., 1399, Budapest Pf 672, Hungary.
authors.forename: Johanna
authors.initials: J
authors.lastname: Mózes
authors.affiliation: Department of Histopathology, School of Medicine, Trinity College Dublin, Dublin 2, Ireland.
authors.forename: Anette
authors.initials: A
authors.lastname: Kovács
authors.affiliation: GenoID Ltd., 1399, Budapest Pf 672, Hungary.
authors.forename: Agnes
authors.initials: A
authors.lastname: Solt
authors.affiliation: Cytology Laboratory, Coombe Women and Infants University Hospital, Dublin 8, Ireland.
authors.forename: Noel
authors.initials: N
authors.lastname: Bolger
authors.affiliation: Department of Histopathology, School of Medicine, Trinity College Dublin, Dublin 2, Ireland; Molecular Pathology Laboratory, Department of Pathology, Coombe Women and Infants University Hospital, Dublin 8, Ireland.
authors.forename: John
authors.initials: J
authors.lastname: O'Leary
authors.affiliation: Department of Histopathology, School of Medicine, Trinity College Dublin, Dublin 2, Ireland; Molecular Pathology Laboratory, Department of Pathology, Coombe Women and Infants University Hospital, Dublin 8, Ireland.
authors.forename: Cara
authors.initials: C
authors.lastname: Martin
mesh_terms: D000328:Adult; D060085:Coinfection / Q000175:diagnosis / Q000821:virology; D005260:Female; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D007494:Ireland; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D013997:Time Factors; D055815:Young Adult
keywords: Colposcopy; GenoID; Genotyping; HC2; HPV; Irish