title: Hydrogen sulfide alleviates lead-induced photosynthetic and ultrastructural changes in oilseed rape.
abstract: The role of hydrogen sulfide (H2S) in alleviating lead (Pb) induced stress in oilseed rape (Brassica napus L.) was studied under laboratory conditions. Plants were grown hydroponically in greenhouse conditions under three levels (0, 100, and 400 µM) of Pb and three levels (0, 100 and 200 µM) of H2S donor, sodium hydrosulfide (NaHS). Application of H2S significantly improved the plant growth, root morphology, chlorophyll contents and photosynthetic activity in leaves of B. napus under Pb stress. Moreover, exogenously applied H2S significantly lowered the Pb concentration in shoots and roots of plants under Pb stress. The microscopic examination indicated that application of exogenous H2S enabled a clean mesophyll cell having a well developed chloroplast with thylakoid membranes and starch grains. A number of modifications could be observed in root tip cell i.e. mature mitochondria, long endoplasmic reticulum and golgibodies under combined application of H2S and Pb. On the basis of these findings, it can be concluded that application of exogenous H2S has a protective role on plant growth, net photosynthesis rate and ultrastructural changes in B. napus plants under high Pb exposures.
authors.affiliation: Institute of Crop Science and Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou 310058, China.
authors.forename: B
authors.initials: B
authors.lastname: Ali
authors.affiliation: Agricultural Technology Extension Center, Zhejiang University, Hangzhou 310058, China.
authors.forename: W J
authors.initials: WJ
authors.lastname: Song
authors.affiliation: Agricultural Experiment Station, Zhejiang University, Hangzhou 310058, China.
authors.forename: W Z
authors.initials: WZ
authors.lastname: Hu
authors.affiliation: Institute of Crop Science and Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou 310058, China; College of Plant Science, Tarim University, Alar, Xinjiang 843300, China.
authors.forename: X N
authors.initials: XN
authors.lastname: Luo
authors.affiliation: Institute of Crop Science and Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou 310058, China.
authors.forename: R A
authors.initials: RA
authors.lastname: Gill
authors.affiliation: Institute of Crop Science and Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou 310058, China.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Crop Science and Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou 310058, China; Agricultural Experiment Station, Zhejiang University, Hangzhou 310058, China. Electronic address: wjzhou@zju.edu.cn.
authors.forename: W J
authors.initials: WJ
authors.lastname: Zhou
mesh_terms: D029688:Brassica napus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D002734:Chlorophyll / Q000378:metabolism; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D018527:Hydroponics; D007854:Lead / Q000633:toxicity*; D046529:Microscopy, Electron, Transmission; D010788:Photosynthesis / Q000187:drug effects*; D018515:Plant Leaves / Q000187:drug effects; D018517:Plant Roots / Q000187:drug effects; D013312:Stress, Physiological / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical; D002734:Chlorophyll; D007854:Lead; D006862:Hydrogen Sulfide
keywords: Brassica napus L.; Hydrogen sulfide; Lead; Plant growth; Root morphology; Ultrastructural changes