title: Investigation on the interaction of pyrene with bovine serum albumin using spectroscopic methods.
abstract: This paper was designed to investigate the interaction of pyrene with bovine serum albumin (BSA) under physiological condition by spectroscopic methods. Spectroscopic analysis of the emission quenching revealed that the quenching mechanism of BSA by pyrene was static. The binding sites and constants of pyrene-BSA complex were observed to be 1.20 and 2.63×10(6) L mol(-1) at 298 K, respectively. The enthalpy change (ΔH) and entropy change (ΔS) revealed that van der Waals forces and hydrogen bonds stabilized the pyrene-BSA complex. Energy transfer from tryptophan to pyrene occurred by a FRET (fluorescence resonance energy transfer) mechanism, and the distance (r=2.72 nm) had been determined. The results of synchronous, three-dimensional fluorescence, and circular dichroism spectra showed that the pyrene induced conformational changes of BSA.
authors.affiliation: School of Environmental Science, Liaoning University, Shenyang 110036, China.
authors.forename: Chengbin
authors.initials: C
authors.lastname: Xu
authors.affiliation: College of Chemistry, Chemical Engineering and Food Safety, Bohai University, Jinzhou 121013, China.
authors.forename: Jiali
authors.initials: J
authors.lastname: Gu
authors.affiliation: School of Environmental Science, Liaoning University, Shenyang 110036, China.
authors.forename: Xiping
authors.initials: X
authors.lastname: Ma
authors.affiliation: School of Environmental Science, Liaoning University, Shenyang 110036, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Dong
authors.affiliation: School of Pharmacy, Liaoning University, Shenyang 110036, China. Electronic address: rubymxl@163.com.
authors.forename: Xuelian
authors.initials: X
authors.lastname: Meng
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002942:Circular Dichroism; D004735:Energy Transfer; D007700:Kinetics; D011721:Pyrenes / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013057:Spectrum Analysis / Q000379:methods*; D013816:Thermodynamics
chemical_list: D011721:Pyrenes; D012710:Serum Albumin, Bovine; C030984:pyrene
keywords: Bovine serum albumin (BSA); Circular dichroism spectra; Fluorescence spectra; Interaction; Pyrene