title: In vivo durability and safety of rolled acellular dermis in a submucosal pocket in pigs.
abstract: Objective : This study explored posterior nasopharyngeal augmentation with an acellular dermal matrix sheeting. Design : Evaluation of the persistence and safety of a submucosal implant of rolled acellular dermis over a 3-month period in a piglet model. Setting : Institute Hills Facility, part of the Mayo Clinic Rochester health care facility. Participants : Fifteen 5-week-old domestic piglets of unspecified gender. Interventions : Twelve piglets were implanted at age 5 weeks with a rolled sheet of acellular dermal matrix (Strattice). Implants were inserted in a submucosal pocket in the soft palate. Three piglets underwent sham operations, with creation of submucosal pockets without implantation. After a 3-month observation period, the palates were harvested for evaluation. Results : Grossly, persistence of bulk at the surgical site in 5 of the 12 implanted piglets was noted at 3 months. Histologically, no persistence of the dermal matrix could be observed. Incorporation and/or resorption of the dermal matrix occurred with minimal to no host inflammatory response. Conclusion : This experiment demonstrated the safety of a rolled acellular dermal implant in a submucosal location in a pig model, without surgical complication, host inflammatory reaction, or rejection. Minimal, if any, bulk of the implant persisted in the implanted location after 3 months. Although acellular dermal matrix sheeting appears to be safe and well-tolerated, it does not offer a long-term treatment option for posterior pharyngeal augmentation.
authors.forename: Ashley G
authors.initials: AG
authors.lastname: O'Reilly
authors.forename: Benjamin D
authors.initials: BD
authors.lastname: Powell
authors.forename: Joaquin J
authors.initials: JJ
authors.lastname: Garcia
authors.forename: Shelagh A
authors.initials: SA
authors.lastname: Cofer
mesh_terms: D062088:Acellular Dermis; D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage*; D003094:Collagen / Q000008:administration & dosage*; D004195:Disease Models, Animal; D006085:Graft Survival; D010160:Palate, Soft / Q000601:surgery*; D019736:Prostheses and Implants; D013552:Swine; D014681:Velopharyngeal Insufficiency / Q000601:surgery
chemical_list: D001672:Biocompatible Materials; C568444:strattice; D003094:Collagen
keywords: cleft palate; posterior pharyngeal augmentation; rolled acellular dermis; velopharyngeal insufficiency