title: Direct detection of human herpesvirus 6B by the LAMP method using newly developed dry-reagents.
abstract: The reliability of the HHV-6B LAMP using the dry-reagent method was evaluated using serum samples obtained from febrile children. The sensitivity of the original and dry-reagent methods was 10 copies/reaction and 100 copies/reaction, respectively. The dry-reagent LAMP method was highly sensitive (94.0%) and specific (96.0%) for the detection of HHV-6B.
authors.affiliation: Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan. Electronic address: tetsushi@fujita-hu.ac.jp.
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Yoshikawa
authors.affiliation: Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Matsuo
authors.affiliation: Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Kawamura
authors.affiliation: Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ohashi
authors.affiliation: Eiken Chemical Co. Ltd., Shimoishigami, Ohtawara, Tochigi, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Yonekawa
authors.affiliation: Eiken Chemical Co. Ltd., Shimoishigami, Ohtawara, Tochigi, Japan.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Kanda
authors.affiliation: Eiken Chemical Co. Ltd., Shimoishigami, Ohtawara, Tochigi, Japan.
authors.forename: Tsugunori
authors.initials: T
authors.lastname: Notomi
authors.affiliation: Faculty of Clinical Engineering, Fujita Health University School of Health Sciences, Toyoake, Aichi, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Ihira
mesh_terms: D005260:Female; D015654:Herpesvirus 6, Human / Q000302:isolation & purification*; D006801:Humans; D007202:Indicators and Reagents; D007223:Infant; D008297:Male; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D019349:Roseolovirus Infections / Q000175:diagnosis* / Q000821:virology*; D012680:Sensitivity and Specificity; D044967:Serum / Q000821:virology
chemical_list: D007202:Indicators and Reagents
keywords: Dry-reagent; Exanthem subitum; HHV-6B; LAMP; Rapid diagnosis