title: [Application of lysosomal detection in marine pollution monitoring: research progress].
abstract: Lysosome is an important organelle existing in eukaryotic cells. With the development of the study on the structure and function of lysosome in recent years, lysosome is considered as a target of toxic substances on subcellular level, and has been widely applied abroad in marine pollution monitoring. This paper summarized the biological characteristics of lysosomal marker enzyme, lysosome-autophagy system, and lysosomal membrane, and introduced the principles and methods of applying lysosomal detection in marine pollution monitoring. Bivalve shellfish digestive gland and fish liver are the most sensitive organs for lysosomal detection. By adopting the lysosomal detection techniques such as lysosomal membrane stability (LMS) test, neutral red retention time (NRRT) assay, morphological measurement (MM) of lysosome, immunohistochemical (Ih) assay of lysosomal marker enzyme, and electron microscopy (EM), the status of marine pollution can be evaluated. It was suggested that the lysosome could be used as a biomarker for monitoring marine environmental pollution. The advantages and disadvantages of lysosomal detection and some problems worthy of attention were analyzed, and the application prospects of lysosomal detection were discussed.
authors.affiliation: Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, Fujian, China. xmwyz0592@163.com
authors.forename: You-Zhu
authors.initials: YZ
authors.lastname: Weng
authors.affiliation: Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, Fujian, China.
authors.forename: Yong-Qiang
authors.initials: YQ
authors.lastname: Fang
authors.affiliation: Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, Fujian, China.
authors.forename: Yu-Sheng
authors.initials: YS
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D049872:Bivalvia / Q000502:physiology; D004784:Environmental Monitoring / Q000379:methods*; D005399:Fishes / Q000502:physiology; D008247:Lysosomes / Q000187:drug effects* / Q000378:metabolism* / Q000502:physiology; D012623:Seawater / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D014874:Water Pollutants, Chemical