title: Methylation pattern of the CD14 and TLR2 genes in human dental pulp.
abstract: BACKGROUND
  Pattern recognition receptors, such as toll-like receptor 2 (TLR-2) and TLR-4, participate in the activation of immune cells by microorganisms in dental pulp. However, the expression levels of pattern recognition receptors can be modulated by epigenetic factors, especially DNA methylation. In this study, the methylation status of the TLR-2 and CD14 (TLR4 co-receptor) genes in healthy and inflamed human dental pulp was examined.
  
  
  METHODS
  The Methyl-Profiler DNA Methylation qPCR Assay was used to verify the DNA methylation patterns.
  
  
  RESULTS
  No differences in the methylation patterns were observed between the 2 groups. Most DNA was unmethylated in both groups.
  
  
  CONCLUSIONS
  The hypomethylation of TLR2 and CD14 genes is a usual feature in human dental pulp.
authors.affiliation: Department of Restorative Dentistry, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Fabiano Pereira
authors.initials: FP
authors.lastname: Cardoso
authors.affiliation: Department of Morphology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Simone Angélica
authors.initials: SA
authors.lastname: de Faria Amormino
authors.affiliation: Department of Morphology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Walderez Ornelas
authors.initials: WO
authors.lastname: Dutra
authors.affiliation: Department of Restorative Dentistry, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Antônio Paulino
authors.initials: AP
authors.lastname: Ribeiro Sobrinho
authors.affiliation: Department of Morphology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. Electronic address: paularocha@ufmg.br.
authors.forename: Paula Rocha
authors.initials: PR
authors.lastname: Moreira
mesh_terms: D000293:Adolescent; D000328:Adult; D018899:CpG Islands / Q000235:genetics; D019175:DNA Methylation / Q000235:genetics* / Q000276:immunology; D003731:Dental Caries / Q000378:metabolism; D003782:Dental Pulp / Q000378:metabolism*; D005260:Female; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011401:Promoter Regions, Genetic / Q000235:genetics; D011671:Pulpitis / Q000378:metabolism; D051195:Toll-Like Receptor 2 / Q000235:genetics*; D055815:Young Adult
chemical_list: D018950:Lipopolysaccharide Receptors; C495342:TLR2 protein, human; D051195:Toll-Like Receptor 2
keywords: CD14; DNA methylation; TLR2; dental pulp