title: A Comparison in Prevalence of Helicobacter pylori in the Gingival Crevicular Fluid from Subjects with Periodontitis and Healthy Individuals using Polymerase Chain Reaction.
abstract: Background and aims. The high prevalence of Helicobacter pylori among the microorganisms isolated from the oral environment brings up the question of whether oral cavity acts as a reservoir for this bacterium. The aim of the present study was to determine and compare the prevalence of H. pylori in gingival crevicular fluid (GCF) of patients with chronic periodontitis (CP) as an infectious disease and healthy subjects using polymerase chain reaction (PCR). Materials and methods. Periodontal examination was performed for all participants. Two sterilized paper points were inserted to the maximum depth of the periodontal pockets of selected teeth. The presence of H. pylori was determined by PCR. In the CP group, the severity of disease was defined as moderate or severe. Further, the frequency of Helicobacter pylori in GCF of each category, and the association between the presence of Helicobacter pylori in GCF and periodontitis were determined. Results. There was no statistically significant association between CP and the presence of H. pylori in the GCF (P = 0.62), there was no significant correlation between the presence of H. pylori in the GCF and gender of the subjects (P = 0.28 in CP group and P = 0.25 in control group), and there was no significant correlation between the presence of H. pylori in the GCF and severity of periodontitis (P = 0.20). Conclusion. Oral cavity acts as a reservoir for H. pylori; however, the results do not show that H. pylori is involved in periodontal disease.
authors.affiliation: Assistant Professor, Department of Oral & Maxillofacial Medicine & Torabinejad Dental Research Center, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Salehi
authors.affiliation: Associate Professor, Department of Periodontics, School of Dentistry & Torabinejad Dental Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Shah Aboei
authors.affiliation: Assistant Professor, Department of Periodontics, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Narges
authors.initials: N
authors.lastname: Naghsh
authors.affiliation: Assistant Professor, Department of Oral & Maxillofacial Medicine, School of Dentistry, Qom University of Medical Sciences, Qom, Iran.
authors.forename: Samira
authors.initials: S
authors.lastname: Hajisadeghi
authors.affiliation: Dentist, Private Practice, Isfahan, Iran.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Ajami
keywords: Helicobacter pylori; PCR; gingival crevicular fluid; periodontal disease