title: Investigation on distribution of airborne fungi in outdoor environment in Tehran, Iran.
abstract: BACKGROUND
  Airborne fungi are responsible for the majority of fungal infections in humans and animals. Outdoor air markedly influences the prevalence of fungal spore levels in indoor air and thus, it is the major source of fungal infections in indoor environments especially in hospitalized individuals.
  
  
  METHODS
  Using a settle plate method, air sampling (1092 air samples from 93 sampling sites in 22 geographic regions of Tehran) was performed by exposing 90 mm settle plates containing Malt extract agar and Potato dextrose agar to the air for 30 min. The plates were incubated at 28°C for 2-3 weeks and examined daily for visible fungal growth. Purified fungal colonies were identified at the genus level based on morphological criteria according to standard methods.
  
  
  RESULTS
  A total of 6455 colonies belonging to 24 different fungal genera were isolated. Area V situated in the city center was the most contaminated region with 2523 fungal colonies (39.1%), while area IV in the West showed the least contamination rate (636 colonies; 9.8%). Airborne fungi isolated were classified into 4 classes including hyaline Hyphomycetes (53.5%), dematiaceous Hyphomycetes (41.6%), Zygomycetes (2.8%) and Coelomycetes (0.2%). Aspergillus (31.3%) was the most prominent isolated fungus followed by Cladosporium (22.1%), Penicillium (13.8%) and Alternaria (12.2%).
  
  
  CONCLUSIONS
  Our results indicate that outdoor air is a potential threat to public health because of harboring a wide array of pathogenic and allergenic airborne fungal spores which can serve as the main source of contamination of indoor environments such as homes, offices and hospitals.
authors.affiliation: Department of Mycology, Faculty of Medical Sciences, Tarbiat Modares University, 14115-331 Tehran, Iran. shamsm@modares.ac.ir.
authors.forename: Masoomeh
authors.initials: M
authors.lastname: Shams-Ghahfarokhi
authors.forename: Sanaz
authors.initials: S
authors.lastname: Aghaei-Gharehbolagh
authors.forename: Narges
authors.initials: N
authors.lastname: Aslani
authors.forename: Mehdi
authors.initials: M
authors.lastname: Razzaghi-Abyaneh