title: Exogenous EDDS modifies copper-induced various toxic responses in rice.
abstract: Copper is a micronutrient required for living organisms, but is potentially toxic in excess. EDDS enhances the phytoextraction of many metals, but the underlying mechanism is fully unclear. Exposure of 200 μM Cu2+ for 3 days resulted in rice seedling growth inhibition, accompanied by a decrease in plasma membrane H+-ATPase activity, and an increase in relative electrolyte leakage ratios, indicating that maintaining of membrane structure integrity is crucial in acclimation of plants to heavy metal stress. In addition, the chlorophyll and carotenoid content was markedly decreased and the level of the mRNA of Cytochrome P450 gene, OsHMA9, the sulfate transporter gene, and the metallothionein-like protein gene was observed to increase in response to Cu stress. Cu treatment also induced a global epigenetic response which is associated with cell nucleus condensation. These physiological, genetic, and epigenetic responses of rice seedlings to excess copper were modified by the addition of EDDS, suggesting that the supply of EDDS in medium containing a high concentration of Cu ions could enhance plant tolerance potential to excess Cu toxicity through alleviating Cu-induced poisonous effects at various levels.
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, People's Republic of China.
authors.forename: Junjun
authors.initials: J
authors.lastname: Tan
authors.forename: Shibin
authors.initials: S
authors.lastname: He
authors.forename: Shihan
authors.initials: S
authors.lastname: Yan
authors.forename: Yingnan
authors.initials: Y
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Zhao
authors.forename: Lijia
authors.initials: L
authors.lastname: Li
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D027321:Anion Transport Proteins / Q000235:genetics; D002338:Carotenoids / Q000378:metabolism; D002462:Cell Membrane / Q000473:pathology*; D002734:Chlorophyll / Q000378:metabolism; D003300:Copper / Q000633:toxicity*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D004573:Electrolytes / Q000378:metabolism; D005029:Ethylenediamines / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant; D006657:Histones / Q000378:metabolism; D008668:Metallothionein / Q000235:genetics / Q000378:metabolism; D008745:Methylation / Q000187:drug effects; D012275:Oryza / Q000187:drug effects / Q000254:growth & development*; D010940:Plant Proteins / Q000235:genetics; D018517:Plant Roots / Q000254:growth & development*; D012333:RNA, Messenger / Q000096:biosynthesis; D036226:Seedlings / Q000254:growth & development / Q000378:metabolism; D013386:Succinates / Q000494:pharmacology*
chemical_list: D027321:Anion Transport Proteins; D004573:Electrolytes; D005029:Ethylenediamines; D006657:Histones; C103078:MT-2 protein, Oryza sativa; D010940:Plant Proteins; D012333:RNA, Messenger; D013386:Succinates; D002734:Chlorophyll; C041447:N,N'-ethylenediamine disuccinic acid; D002338:Carotenoids; D003300:Copper; D003577:Cytochrome P-450 Enzyme System; D008668:Metallothionein; D000251:Adenosine Triphosphatases