title: Immunogenicity and protective efficacy of yeast extracts containing rotavirus-like particles: a potential veterinary vaccine.
abstract: Rotavirus is the most common cause of severe diarrhea in many animal species of economic interest. A simple, safe and cost-effective vaccine is required for the control and prevention of rotavirus in animals. In this study, we evaluated the use of Saccharomyces cerevisiae extracts containing rotavirus-like particles (RLP) as a vaccine candidate in an adult mice model. Two doses of 1mg of yeast extract containing rotavirus proteins (between 0.3 and 3 μg) resulted in an immunological response capable of reducing the replication of rotavirus after infection. Viral shedding in all mice groups diminished in comparison with the control group when challenged with 100 50% diarrhea doses (DD50) of murine rotavirus strain EDIM. Interestingly, when immunizing intranasally protection against rotavirus infection was observed even when no increase in rotavirus-specific antibody titers was evident, suggesting that cellular responses were responsible of protection. Our results indicate that raw yeast extracts containing rotavirus proteins and RLP are a simple, cost-effective alternative for veterinary vaccines against rotavirus.
authors.affiliation: Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, CP 62250 Cuernavaca, Morelos, Mexico.
authors.forename: William A
authors.initials: WA
authors.lastname: Rodríguez-Limas
authors.affiliation: Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, CP 62250 Cuernavaca, Morelos, Mexico.
authors.forename: Ana Ruth
authors.initials: AR
authors.lastname: Pastor
authors.affiliation: Facultad de Medicina, Universidad Autónoma del Estado de Morelos, CP 62350 Cuernavaca, Morelos, Mexico.
authors.forename: Ernesto
authors.initials: E
authors.lastname: Esquivel-Soto
authors.affiliation: Facultad de Medicina, Universidad Autónoma del Estado de Morelos, CP 62350 Cuernavaca, Morelos, Mexico.
authors.forename: Fernando
authors.initials: F
authors.lastname: Esquivel-Guadarrama
authors.affiliation: Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, CP 62250 Cuernavaca, Morelos, Mexico.
authors.forename: Octavio T
authors.initials: OT
authors.lastname: Ramírez
authors.affiliation: Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, CP 62250 Cuernavaca, Morelos, Mexico. Electronic address: laura@ibt.unam.mx.
authors.forename: Laura A
authors.initials: LA
authors.lastname: Palomares
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000917:Antibody Formation; D000956:Antigens, Viral / Q000276:immunology; D061249:Batch Cell Culture Techniques; D036022:Capsid Proteins / Q000276:immunology; D005260:Female; D051379:Mice; D012401:Rotavirus; D012400:Rotavirus Infections / Q000517:prevention & control*; D022243:Rotavirus Vaccines / Q000276:immunology*; D012441:Saccharomyces cerevisiae / Q000276:immunology / Q000821:virology*; D058425:Vaccines, Virus-Like Particle / Q000276:immunology*; D014776:Virus Cultivation; D017201:Virus Shedding
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D022243:Rotavirus Vaccines; C442545:VP2 protein, Rotavirus; C061332:VP6 protein, Rotavirus; C050779:VP7 protein, Rotavirus; D058425:Vaccines, Virus-Like Particle
keywords: Rotavirus; Vaccines for bovines, Virus-like particles, Saccharomyces cerevisiae; Yeast