title: Comparative analysis of the venom proteome of four important Malaysian snake species.
abstract: BACKGROUND
  Naja kaouthia, Ophiophagus hannah, Bungarus fasciatus and Calloselasma rhodostoma are four venomous snakes indigenous to Malaysia. In the present study, their proteomic profile by two-dimensional gel electrophoresis (2-DE) have been separated and compared.
  
  
  RESULTS
  The 2-DE of venoms of the four species snake demonstrated complexity and obvious interspecies differences in proteome profiles. A total of 63 proteins were identified in the four species: C. rhodostoma - 26, N. kaouthia - 16, O. hannah - 15 and B. fasciatus - 6.
  
  
  CONCLUSIONS
  Despite the identifications of major proteins in the four snake species, a large number of protein spots from the 2-DE were unidentified even though the spots displayed high-quality MALDI-TOF-MS spectra. Those identified included phospholipase A2 proteins in all four venoms, long neurotoxins in both cobra species and the C. rhodostoma venom found with the most varied types of peptidases, i.e. metalloproteinase kistomin, halystase and L-amino acid oxidase.
authors.affiliation: Faculty of Industrial Sciences and Technology, University Malaysia Pahang, Lebuhraya Tun Razak, Kuantan, Pahang Darul Makmur 26300, Malaysia. jayavejayan@yahoo.com.
authors.forename: Jaya
authors.initials: J
authors.lastname: Vejayan
authors.forename: Too Lay
authors.initials: TL
authors.lastname: Khoon
authors.forename: Halijah
authors.initials: H
authors.lastname: Ibrahim