title: Post-maturation zona perforation improves porcine parthenogenetic trophoblast culture.
abstract: This study was designed to optimize a method to improve porcine parthenogenetic embryo hatching and trophoblast culture. Mature oocytes (D0PPA) and day 6 blastocysts (D6PPA) were perforated with a 20 μm diameter needle for assisted hatching. The two groups showed a significant difference in hatching rate and blastocyst cell doubling when compared to a non-perforated control group. D0PPA blastocysts were able to form tertiary trophoblast colonies but D6PPA and control groups were not able to grow beyond primary colonies. Quantitative real-time PCR analysis showed significant differences in BAX, BAX/BCL2L1 and HSP70-2 mRNA expression between the experimental groups.
authors.affiliation: Department of Theriogenology and Biotechnology, College of Veterinary Medicine and The Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea; Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt.
authors.forename: I M
authors.initials: IM
authors.lastname: Saadeldin
authors.affiliation: Department of Theriogenology and Biotechnology, College of Veterinary Medicine and The Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: S J
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Department of Theriogenology and Biotechnology, College of Veterinary Medicine and The Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Y B
authors.initials: YB
authors.lastname: Choi
authors.affiliation: Department of Theriogenology and Biotechnology, College of Veterinary Medicine and The Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea; Institute of Green Bio Science and Technology, Seoul National University, Pyeong Chang, Kangwon do, Republic of Korea. Electronic address: bclee@snu.ac.kr.
authors.forename: B C
authors.initials: BC
authors.lastname: Lee
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D046149:Embryo Culture Techniques; D005260:Female; D009865:Oocytes; D010312:Parthenogenesis; D013552:Swine; D014327:Trophoblasts
keywords: Parthenogenesis; Pig; Trophoblast outgrowth; Zona perforation