title: Comparison of sagittal and transverse echo planar spectroscopic imaging on the quantification of brain metabolites.
abstract: OBJECTIVE
  We quantitatively compared sagittal and transverse echo planar spectroscopic imaging (EPSI) on the quantification of metabolite concentrations with consideration of tissue variation. A quantification strategy is proposed to collect the necessary information for quantification of concentrations in a minimized acquisition time.
  
  
  METHODS
  Six transverse and six sagittal EPSI data were collected on healthy volunteers. Metabolite concentrations of N-acetyl-aspartate (NAA), total creatine (tCr), total choline (tCho), myo-inositol (mI), and glutamate and glutamine complex (Glx) were quantified using water scaling with partial volume and relaxation correction. Linear regression analysis was performed to extract concentrations in gray matter (GM) and white matter (WM). The inter- and intrasubject coefficients of variance (CV) were estimated.
  
  
  RESULTS
  Concentrations and fitting errors of sagittal and transverse EPSI were at same level. GM to WM contrast of concentrations was found in NAA, tCr, and tCho. The intersubject CVs revealed greater variability in the sagittal EPSI than in the transverse EPSI. The intrasubject CVs of the transverse EPSI were below 5% for NAA, tCr, and tCho.
  
  
  CONCLUSIONS
  We showed that quantified concentrations of sagittal and transverse EPSI after partial volume correction are comparable and reproducible. The proposed quantification strategy can be conveniently adapted into various MRI protocols.
authors.affiliation: Graduate Institute of Applied Physics, National Chengchi University, Taipei, Taiwan.|Mind, Brain and Learning Center, National Chengchi University, Taipei, Taiwan.
authors.forename: Shang-Yueh
authors.initials: SY
authors.lastname: Tsai
authors.affiliation: Graduate Institute of Applied Physics, National Chengchi University, Taipei, Taiwan.
authors.forename: Woan-Chyi
authors.initials: WC
authors.lastname: Wang
authors.affiliation: Department of Electronic and Computer Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan.
authors.forename: Yi-Ru
authors.initials: YR
authors.lastname: Lin
mesh_terms: D000328:Adult; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism*; D017352:Echo-Planar Imaging / Q000379:methods*; D005260:Female; D006801:Humans; D057054:Molecular Imaging / Q000379:methods*; D056888:Patient Positioning / Q000379:methods*; D066244:Proton Magnetic Resonance Spectroscopy / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D055815:Young Adult
keywords: Magnetic resonance spectroscopic imaging; brain metabolites; echo planar spectroscopic imaging; partial volume correction