title: Anesthetic effect of a combination of medetomidine-midazolam-butorphanol in cynomolgus monkeys (Macaca fascicularis).
abstract: The anesthetic effect of a combination of medetomidine, midazolam and butorphanol (Me-Mi-Bu) was evaluated in healthy cynomolgus monkeys. The Me-Mi-Bu combination was intramuscularly administered as follows: Dose 1, Me 0.015 mg/kg-Mi 0.1 mg/kg-Bu 0.15 mg/kg; Dose 2, Me 0.02 mg/kg-Mi 0.15 mg/kg-Bu 0.2 mg/kg; and Dose 3, Me 0.04 mg/kg-Mi 0.3 mg/kg-Bu 0.4 mg/kg. The combination rapidly induced immobilization, and lateral recumbency was reached within 15 min. The duration of anesthesia for each dose administered was follows: Dose 1, 47 ± 27 min; Dose 2, 113 ± 31 min; and Dose 3, 190 ± 24 min. The anesthetic effect of the combination was abolished by the α2-adrenoceptor antagonist atipamezole. No marked changes in the levels of hematologic or serum biochemical parameters were noted in cynomolgus monkeys administered the combination plus atipamezole. Taken together, these results suggest that the Me-Mi-Bu combination exhibits reversible anesthetic effect and may be useful for studies involving cynomolgus monkeys.
authors.affiliation: Laboratory Animal Science Div., Astellas Research Technologies Co., Ltd., 1-6 Kashima 2-chome, Yodogawa-ku, Osaka 532-8514, Japan.
authors.forename: Takehiro
authors.initials: T
authors.lastname: Ochi
authors.forename: Ippei
authors.initials: I
authors.lastname: Nishiura
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Tatsumi
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Hirano
authors.forename: Kouichi
authors.initials: K
authors.lastname: Yahagi
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Sakurai
authors.forename: Kanako
authors.initials: K
authors.lastname: Matsuyama-Fujiwara
authors.forename: Yuji
authors.initials: Y
authors.lastname: Sudo
authors.forename: Noriko
authors.initials: N
authors.lastname: Nishina
authors.forename: Hironari
authors.initials: H
authors.lastname: Koyama
mesh_terms: D000758:Anesthesia / Q000662:veterinary*; D000777:Anesthetics / Q000494:pharmacology*; D019162:Anesthetics, Combined / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D002077:Butorphanol / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007273:Injections, Intramuscular / Q000662:veterinary; D008252:Macaca fascicularis / Q000502:physiology*; D020926:Medetomidine / Q000008:administration & dosage / Q000494:pharmacology*; D008874:Midazolam / Q000008:administration & dosage / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D000777:Anesthetics; D019162:Anesthetics, Combined; D020926:Medetomidine; D002077:Butorphanol; D008874:Midazolam