title: Impact of aortic cross-clamping time on peripheral nerves: experimental model.
abstract: OBJECTIVE
  The present study investigated the correlation between extend aortic cross-clamping time and peripheral nerve injury on rats.
  
  
  METHODS
  24 male, Sprague Dawley rats were divided into 3 groups; (a) control group: abdomen was directly closed after reached aorta, and followed by 72 hours, (b) short-term ischaemia-reperfusion group: peripheral nerve ischemia was induced in rats by supraceliac aortic occlusion for 20 min followed by 72 h of reperfusion, (c) long-term ischaemia-reperfusion group: peripheral nerve ischemia was induced for 30 min followed by 72 h of reperfusion. Preoperative and postoperative, electromyography (EMG) recordings were done. End of 72 h, the sciatic nerves were harvested from each animal for histopathological and biochemical analysis.
  
  
  RESULTS
  The mean compound muscle action potential (CMAP) amplitude of long-term ischaemia-reperfusion group was statically significant reduced when compared to the control group (p <0.01). However, the mean distal latency value of long-term ischaemia-reperfusion group was statically significant increased (p <0.01). On the other hand, there were statically significant differences between the results of malondialdehyde, edema and ischemia fiber degeneration grades on control and long-term ischaemia-reperfusion group (p <0.001).
  
  
  CONCLUSIONS
  This study demonstrated that the extending cross clamping time directly harms the peripheral nerve of rats.
authors.affiliation: Department of Plastic, Reconstructive and Aesthetic Surgery, Medikalpark Hospital, Istanbul, Turkey.
authors.forename: Ovunc
authors.initials: O
authors.lastname: Akdemir
authors.forename: Ilknur
authors.initials: I
authors.lastname: Akdemir
authors.forename: Turker
authors.initials: T
authors.lastname: Cavusoglu
authors.forename: William C
authors.initials: WC
authors.lastname: Lineaweaver
authors.forename: Utku
authors.initials: U
authors.lastname: Ates
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Oytun
authors.initials: O
authors.lastname: Erbas
mesh_terms: D000200:Action Potentials; D000818:Animals; D001011:Aorta / Q000601:surgery*; D003250:Constriction; D004487:Edema / Q000209:etiology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D023421:Models, Animal; D009410:Nerve Degeneration; D061646:Operative Time; D059348:Peripheral Nerve Injuries / Q000209:etiology* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D017207:Rats, Sprague-Dawley; D011930:Reaction Time; D015427:Reperfusion Injury / Q000209:etiology* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D012584:Sciatic Nerve / Q000293:injuries* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D013997:Time Factors
chemical_list: D008315:Malondialdehyde