title: Changes in thyroid hormone receptors after permanent cerebral ischemia in male rats.
abstract: Thyroid hormones (TH) and receptors (TRs) may play an important role in the pathophysiology of acute cerebral ischemia. In the present study, we sought to determine whether serum triodothyronine (T3)/thyroxine (T4) and brain TRs (TRα1, TRβ1) might change after experimental stroke. Male adult Wistar rats were subjected to permanent middle cerebral artery occlusion (group P) and compared to sham-operated controls (group S). Animals were followed clinically for 14 days until brain collection for Western blot (WB) or neuropathological analysis of TRs in three different brain areas (infarcted tissue, E1; noninfarcted ipsilateral hemisphere, E2; and contralateral hemisphere, E3). Analysis of serum TH levels showed a reduction of T4 in group P (p = 0.002) at days 2 to 14, while half of the animals also displayed "low T3" values (p = 0.012) on day 14. This T4 reduction was inversely correlated to the clinical severity of stroke and the concomitant body weight loss (p < 0.005). WB analysis of TRα1 and TRβ1 protein expression showed heterogenic responses at day 14: total and nuclear TRα1 were similar between the two groups, while total TRβ1 decreased 7.5-fold within E1 (p ≤ 0.001) with a concomitant 1.8-fold increase of nuclear TRβ1 in E2 area (p = 0.03); TRβ1 expression did not differ in E3. Neuropathological analysis revealed that activated macrophages/microglia exclusively expressed nuclear TRα1 within the infarct core. Astrocytes mildly expressed nuclear TRα1 in and around the infarct, along with a prominent TRβ nuclear signal restricted in the astrocytic scar. Neurons around the infarct expressed mainly TRα1 and, to a milder degree, TRβ. Surprisingly enough, we detected for the first time a TRβ expression in the paranodal region of Ranvier nodes, of unknown significance so far. Our data support that cerebral ischemia induces a low TH response, associated with significant and heterogenic changes in brain TR expression. These findings could imply an important role of TH signaling in cerebral ischemia.
authors.affiliation: B' Department of Neurology, Laboratory of Experimental Neurology and Neuroimmunology, AHEPA University Hospital, Stilponos Kiriakides str. 1, 54636, Thessaloniki, Macedonia, Greece.
authors.forename: Athanasios
authors.initials: A
authors.lastname: Lourbopoulos
authors.forename: Iordanis
authors.initials: I
authors.lastname: Mourouzis
authors.forename: Theodoros
authors.initials: T
authors.lastname: Karapanayiotides
authors.forename: Evangelia
authors.initials: E
authors.lastname: Nousiopoulou
authors.forename: Stavros
authors.initials: S
authors.lastname: Chatzigeorgiou
authors.forename: Theodoros
authors.initials: T
authors.lastname: Mavridis
authors.forename: Ioannis
authors.initials: I
authors.lastname: Kokkinakis
authors.forename: Olga
authors.initials: O
authors.lastname: Touloumi
authors.forename: Theano
authors.initials: T
authors.lastname: Irinopoulou
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Chouliaras
authors.forename: Constantinos
authors.initials: C
authors.lastname: Pantos
authors.forename: Dimitris
authors.initials: D
authors.lastname: Karacostas
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Grigoriadis
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D020244:Infarction, Middle Cerebral Artery / Q000378:metabolism*; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D008297:Male; D017628:Microglia / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D009928:Organ Specificity; D051381:Rats; D017208:Rats, Wistar; D037021:Thyroid Hormone Receptors alpha / Q000235:genetics / Q000378:metabolism*; D037042:Thyroid Hormone Receptors beta / Q000235:genetics / Q000378:metabolism*; D013963:Thyroid Hormones / Q000097:blood
chemical_list: D037021:Thyroid Hormone Receptors alpha; D037042:Thyroid Hormone Receptors beta; D013963:Thyroid Hormones