title: Effect of diabetic osteoblasts on osteogenic differentiation of human umbilical cord mesenchymal stem cells.
abstract: BACKGROUND
  This study was aimed to investigate whether osteoblasts from diabetic patients have a promoting effect on osteogenesis of human umbilical cord mesenchymal stem cells (HUMSCs).
  
  
  METHODS
  HUMSCs were co-cultured with osteoblasts of diabetic and non-diabetic patients. Morphological appearance and cytochemical characteristics of the non-diabetic osteoblasts and diabetic osteoblasts were observed by hematoxylin-eosin staining, type I collagen protein expression, alkaline phosphatase (ALP) staining and Alizarin Red S staining. Cell viability, type I collagen protein expression, ALP activity and osteocalcin mRNA expression in HUMSCs were investigated.
  
  
  RESULTS
  Compared with negative control group, the cell proliferation, type I collagen protein expression, ALP activity and osteocalcin mRNA were increased in HUMSCs co-cultured with diabetic and non-diabetic osteoblasts (P<0.05). There was no statistically significant difference in the HUMSCs cell proliferation, type I collagen protein expression, ALP activity and osteocalcin mRNA between the non-diabetic and diabetic group (P >0.05).
  
  
  CONCLUSIONS
  Similar to osteoblasts from non-diabetic patients, osteoblasts from diabetic patients also have the ability to promote HUMSCs proliferation, and leading to HUMSCs exhibit some characteristic of osteoblasts.
authors.affiliation: Department of Orthopedics, Liaocheng People's Hospital, Liaocheng, Shandong, 252000, China.
authors.forename: Cheng-Zhi
authors.initials: CZ
authors.lastname: Ha
authors.affiliation: Oral Maxillofacial-Head and Neck Key Laboratory of Medical Biology, Liaocheng People's Hospital, Liaocheng, Shandong, 252000, China.
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Chen
authors.affiliation: Department of Stomatology, Liaocheng People's Hospital, Liaocheng, Shandong, 252000, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Oral Maxillofacial-Head and Neck Key Laboratory of Medical Biology, Liaocheng People's Hospital, Liaocheng, Shandong, 252000, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Oral Maxillofacial-Head and Neck Key Laboratory of Medical Biology, Liaocheng People's Hospital, Liaocheng, Shandong, 252000, China.
authors.forename: Ying-Xin
authors.initials: YX
authors.lastname: Zhang
authors.affiliation: Oral Maxillofacial-Head and Neck Key Laboratory of Medical Biology, Liaocheng People's Hospital, Liaocheng, Shandong, 252000, China.
authors.forename: Li
authors.initials: L
authors.lastname: Pan
authors.affiliation: Oral Maxillofacial-Head and Neck Key Laboratory of Medical Biology, Liaocheng People's Hospital, Liaocheng, Shandong, 252000, China.
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Wang
authors.affiliation: Oral Maxillofacial-Head and Neck Key Laboratory of Medical Biology, Liaocheng People's Hospital, Liaocheng, Shandong, 252000, China.
authors.forename: Shuang-Feng
authors.initials: SF
authors.lastname: Chen
authors.affiliation: Department of Orthopedics, Liaocheng People's Hospital, Liaocheng, Shandong, 252000, China.|School of Biomedical Sciences, Charles Sturt University, Wagga Wagga, NSW 2650, Australia.
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Wang
authors.affiliation: School of Biomedical Sciences, Charles Sturt University, Wagga Wagga, NSW 2650, Australia.
authors.forename: Le-Xin
authors.initials: LX
authors.lastname: Wang
mesh_terms: D000368:Aged; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D003920:Diabetes Mellitus / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D010006:Osteoblasts / Q000378:metabolism / Q000473:pathology*; D010012:Osteogenesis
keywords: bone healing; diabetic; human umbilical cord; mesenchymal stem cells; osteoblasts