title: Ca2+-binding motif of βγ-crystallins.
abstract: βγ-Crystallin-type double clamp (N/D)(N/D)XX(S/T)S motif is an established but sparsely investigated motif for Ca(2+) binding. A βγ-crystallin domain is formed of two Greek key motifs, accommodating two Ca(2+)-binding sites. βγ-Crystallins make a separate class of Ca(2+)-binding proteins (CaBP), apparently a major group of CaBP in bacteria. Paralleling the diversity in βγ-crystallin domains, these motifs also show great diversity, both in structure and in function. Although the expression of some of them has been associated with stress, virulence, and adhesion, the functional implications of Ca(2+) binding to βγ-crystallins in mediating biological processes are yet to be elucidated.
authors.affiliation: Centre for Cellular and Molecular Biology (CCMB), Council of Scientific and Industrial Research (CSIR), Uppal Road, Hyderabad-500 007, India.
authors.forename: Shanti Swaroop
authors.initials: SS
authors.lastname: Srivastava
authors.affiliation: Centre for Cellular and Molecular Biology (CCMB), Council of Scientific and Industrial Research (CSIR), Uppal Road, Hyderabad-500 007, India.
authors.forename: Amita
authors.initials: A
authors.lastname: Mishra
authors.affiliation: Centre for Cellular and Molecular Biology (CCMB), Council of Scientific and Industrial Research (CSIR), Uppal Road, Hyderabad-500 007, India.
authors.forename: Bal
authors.initials: B
authors.lastname: Krishnan
authors.affiliation: Centre for Cellular and Molecular Biology (CCMB), Council of Scientific and Industrial Research (CSIR), Uppal Road, Hyderabad-500 007, India. Electronic address: yogendra@ccmb.res.in.
authors.forename: Yogendra
authors.initials: Y
authors.lastname: Sharma
mesh_terms: D020816:Amino Acid Motifs; D001419:Bacteria / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002118:Calcium / Q000737:chemistry* / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D038204:beta-Crystallins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D038222:gamma-Crystallins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002135:Calcium-Binding Proteins; D038204:beta-Crystallins; D038222:gamma-Crystallins; D002118:Calcium
keywords: Beta Gamma-Crystallin; Calcium; Calcium-binding Proteins; Crystallin; Greek Key Motif; Protein Stability; Protein Structure