title: Activation of matrix metalloproteinase-26 by HOXA10 promotes embryo adhesion in vitro.
abstract: Successful embryonic implantation requires an effective maternal-embryonic molecular dialogue. However, the detailed mechanisms of epithelial-embryo adhesion remain poorly understood. Here, we report that matrix metalloproteinase-26 (MMP-26) is a novel downstream target gene of homeobox a 10 (HOXA10) in human endometrial cells. HOXA10 binds directly to a conserved TTAT unit (-442 to -439) located within the 5' regulatory region of the MMP-26 gene and regulates the expression and secretion of MMP-26 in a concentration-dependent manner. Moreover, the adenovirus-mediated overexpression of MMP-26 in Ishikawa cells markedly increased BeWo spheroid adhesion. An antibody blocking assay further demonstrated that the promotion of BeWo spheroid adhesion by HOXA10 and MMP-26 was significantly inhibited by pre-treatment with a specific antibody against MMP-26. These results demonstrate that the HOXA10-mediated expression of MMP-26 promotes embryo adhesion during the process of embryonic implantation.
authors.affiliation: Reproductive Medicine Center, Drum Tower Clinic Medical College of Nanjing Medical University, Nanjing 210029, Jiangsu, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Reproductive Medicine Center, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, Jiangsu, China.
authors.forename: Guijun
authors.initials: G
authors.lastname: Yan
authors.affiliation: Reproductive Medicine Center, Drum Tower Clinic Medical College of Nanjing Medical University, Nanjing 210029, Jiangsu, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Reproductive Medicine Center, Drum Tower Clinic Medical College of Nanjing Medical University, Nanjing 210029, Jiangsu, China.
authors.forename: Huizhi
authors.initials: H
authors.lastname: Shan
authors.affiliation: Reproductive Medicine Center, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, Jiangsu, China.
authors.forename: Chengcai
authors.initials: C
authors.lastname: Kong
authors.affiliation: Reproductive Medicine Center, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, Jiangsu, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yan
authors.affiliation: Reproductive Medicine Center, Drum Tower Clinic Medical College of Nanjing Medical University, Nanjing 210029, Jiangsu, China.
authors.forename: Bai
authors.initials: B
authors.lastname: Xue
authors.affiliation: Reproductive Medicine Center, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, Jiangsu, China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Diao
authors.affiliation: Reproductive Medicine Center, Drum Tower Clinic Medical College of Nanjing Medical University, Nanjing 210029, Jiangsu, China. Electronic address: yali_hu@hotmail.com.
authors.forename: Yali
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Reproductive Medicine Center, Drum Tower Clinic Medical College of Nanjing Medical University, Nanjing 210029, Jiangsu, China. Electronic address: stevensunz@163.com.
authors.forename: Haixiang
authors.initials: H
authors.lastname: Sun
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D010064:Embryo Implantation; D004717:Endometrium / Q000166:cytology*; D004789:Enzyme Activation; D005260:Female; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D053505:Matrix Metalloproteinases, Secreted / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011485:Protein Binding
chemical_list: D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins; C069634:HOXA10 protein, human; C410599:MMP26 protein, human; D053505:Matrix Metalloproteinases, Secreted
keywords: Embryo adhesion; HOXA10; MMP-26