title: Assaying mRNA deadenylation in vitro.
abstract: Deadenylation is the removal of poly(A) tails from mRNA. This chapter presents two methods to assay deadenylation in vitro. The first is a quick and quantitative assay for the degradation of radiolabeled poly(A) that can easily be adapted to be used for many different enzymes. The second method uses an extract from Drosophila embryos to catalyze the deadenylation of an RNA dependent on a specific sequence that also directs deadenylation in vivo.
authors.affiliation: Institute of Biochemistry and Biotechnology, Martin Luther University Halle-Wittenberg, Kurt-Mothes-Strasse 3, 06120, Halle, Germany.
authors.forename: Mandy
authors.initials: M
authors.lastname: Jeske
authors.forename: Claudia
authors.initials: C
authors.lastname: Temme
authors.forename: Elmar
authors.initials: E
authors.lastname: Wahle
mesh_terms: D000818:Animals; D004330:Drosophila / Q000235:genetics; D011061:Poly A / Q000378:metabolism*; D012333:RNA, Messenger / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D011061:Poly A