title: Doxorubicin-induced oxidative stress in rats is efficiently counteracted by dietary anthocyanin differently enriched strawberry (Fragaria × ananassa Duch.).
abstract: This study investigated the effects of two different strawberry cultivars, Adria and Sveva, against doxorubicin (DOX)-induced toxicity in rats. A controlled dietary intervention was conducted over 16 weeks with four groups: (i) normal diet; (ii) normal diet + DOX injection; (iii) Adria supplementation + DOX injection; and (iv) Sveva supplementation + DOX injection. Sveva presented higher total antioxidant capacity value and phenol and and vitamin C levels than Adria, which in turn presented higher anthocyanin contents. DOX drastically increased lymphocyte DNA damage, liver biomarkers of protein and lipid oxidation, and mitochondrial ROS content and markedly decreased plasma retinol level, liver antioxidant enzymes, and mitochondrial functionality. After 2 months of strawberry supplementation, rats presented a significant reduction of DNA damage and ROS concentration and a significant improvement of oxidative stress biomarkers, antioxidant enzyme activities, and mitochondrial performance. These results suggest that strawberry supplementation can counteract DOX toxicity, confirming the potential health benefit of strawberry in vivo against oxidative stress.
authors.affiliation: Department of Agriculture, Food and Environmental Science, Marche Polytechnic University , 60121 Ancona, Italy.
authors.forename: Jacopo
authors.initials: J
authors.lastname: Diamanti
authors.forename: Bruno
authors.initials: B
authors.lastname: Mezzetti
authors.forename: Francesca
authors.initials: F
authors.lastname: Giampieri
authors.forename: José M
authors.initials: JM
authors.lastname: Alvarez-Suarez
authors.forename: José L
authors.initials: JL
authors.lastname: Quiles
authors.forename: Adrian
authors.initials: A
authors.lastname: Gonzalez-Alonso
authors.forename: Maria del Carmen
authors.initials: Mdel C
authors.lastname: Ramirez-Tortosa
authors.forename: Sergio
authors.initials: S
authors.lastname: Granados-Principal
authors.forename: Ana M
authors.initials: AM
authors.lastname: Gonzáles-Paramás
authors.forename: Celestino
authors.initials: C
authors.lastname: Santos-Buelga
authors.forename: Maurizio
authors.initials: M
authors.lastname: Battino
mesh_terms: D000818:Animals; D000872:Anthocyanins / Q000378:metabolism*; D056486:Chemical and Drug Induced Liver Injury / Q000178:diet therapy* / Q000209:etiology / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects; D004317:Doxorubicin / Q000633:toxicity*; D031985:Fragaria / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000872:Anthocyanins; D004317:Doxorubicin
keywords: DNA damage; anthocyanins; doxorubicin; mitochondrial functionality; oxidative stress; strawberry