title: Low-copy number protein detection by electrode nanogap-enabled dielectrophoretic trapping for surface-enhanced Raman spectroscopy and electronic measurements.
abstract: We report a versatile analysis platform, based on a set of nanogap electrodes, for the manipulation and sensing of biomolecules, as demonstrated here for low-copy number protein detection. An array of Ti nanogap electrode with sub-10 nm gap size function as templates for alternating current dielectrophoresis-based molecular trapping, hot spots for surface-enhanced Raman spectroscopy as well as electronic measurements, and fluorescence imaging. During molecular trapping, recorded Raman spectra, conductance measurements across the nanogaps, and fluorescence imaging show unambiguously the presence and characteristics of the trapped proteins. Our platform opens up a simple way for multifunctional low-concentration heterogeneous sample analysis without the need for target preconcentration.
authors.affiliation: Nanoscience and Technology Program, Taiwan International Graduate Program, Academia Sinica , Taipei 11529, Taiwan.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Lesser-Rojas
authors.forename: Petra
authors.initials: P
authors.lastname: Ebbinghaus
authors.forename: Ganesh
authors.initials: G
authors.lastname: Vasan
authors.forename: Ming-Lee
authors.initials: ML
authors.lastname: Chu
authors.forename: Andreas
authors.initials: A
authors.lastname: Erbe
authors.forename: Chia-Fu
authors.initials: CF
authors.lastname: Chou
mesh_terms: D004581:Electronics; D036103:Nanotechnology / Q000379:methods*; D061848:Optical Imaging; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D013059:Spectrum Analysis, Raman; D013499:Surface Properties
chemical_list: D011506:Proteins