title: Drug discovery through stem cell-based organoid models.
abstract: The development of new drugs is currently a long and costly process in large part due to the failure of promising drug candidates identified in initial in vitro screens to perform as intended in vivo. New approaches to drug screening are being developed which focus on providing more biomimetic platforms. This review surveys this new generation of drug screening technologies, and provides an overview of recent developments in organoid culture systems which could afford previously unmatched fidelity for testing bioactivity and toxicity. The challenges inherent in such approaches will also be discussed, with a view towards bridging the gap between proof-of-concept studies and a wider implementation within the drug development community.
authors.affiliation: Laboratory of Stem Cell Bioengineering, Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), Switzerland. Electronic address: adrian.ranga@epfl.ch.
authors.forename: Adrian
authors.initials: A
authors.lastname: Ranga
authors.affiliation: Laboratory of Stem Cell Bioengineering, Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), Switzerland.
authors.forename: Nikolche
authors.initials: N
authors.lastname: Gjorevski
authors.affiliation: Laboratory of Stem Cell Bioengineering, Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), Switzerland. Electronic address: matthias.lutolf@epfl.ch.
authors.forename: Matthias P
authors.initials: MP
authors.lastname: Lutolf
mesh_terms: D000818:Animals; D055808:Drug Discovery / Q000379:methods / Q000639:trends*; D004353:Drug Evaluation, Preclinical / Q000379:methods / Q000639:trends; D064420:Drug-Related Side Effects and Adverse Reactions / Q000175:diagnosis / Q000517:prevention & control; D006801:Humans; D009940:Organoids / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D013234:Stem Cells / Q000187:drug effects* / Q000502:physiology*
keywords: 3D cell culture; Drug discovery; High-throughput screening; Organoid; Stem cell