title: A partial proteome reference map of the wine lactic acid bacterium Oenococcus oeni ATCC BAA-1163.
abstract: Oenococcus oeni is the main lactic acid bacterium that carries out the malolactic fermentation in virtually all red wines and in some white and sparkling wines. Oenococcus oeni possesses an array of metabolic activities that can modify the taste and aromatic properties of wine. There is, therefore, industrial interest in the proteins involved in these metabolic pathways and related transport systems of this bacterium. In this work, we report the characterization of the O. oeni ATCC BAA-1163 proteome. Total and membrane protein preparations from O. oeni were standardized and analysed by two-dimensional gel electrophoresis. Using tandem mass spectrometry, we identified 224 different spots corresponding to 152 unique proteins, which have been classified by their putative function and subjected to bioinformatics analysis.
authors.affiliation: Departamento de Microbiología Molecular y Biología de las Infecciones, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Calle Ramiro de Maeztu 9, Madrid 28040, Spain.
authors.forename: María de la Luz
authors.initials: Mde L
authors.lastname: Mohedano
authors.forename: Pasquale
authors.initials: P
authors.lastname: Russo
authors.forename: Vivian
authors.initials: V
authors.lastname: de Los Ríos
authors.forename: Vittorio
authors.initials: V
authors.lastname: Capozzi
authors.forename: Pilar
authors.initials: P
authors.lastname: Fernández de Palencia
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Spano
authors.forename: Paloma
authors.initials: P
authors.lastname: López
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D019295:Computational Biology; D015180:Electrophoresis, Gel, Two-Dimensional; D019344:Lactic Acid / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D056585:Oenococcus / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D053719:Tandem Mass Spectrometry; D014920:Wine / Q000032:analysis*
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D020543:Proteome; D019344:Lactic Acid
keywords: Oenococcus oeni; proteome; two-dimensional electrophoresis