title: Recovery of Green Plantlets from Albino Shoot Primordia Derived from Anther Culture of Indica Rice (Oryza sativa L.).
abstract: A simple method was developed to permit albino plant regeneration from anther culture of Hobigonj Boro (Hbj B) IV and Hbj B VI, two local varieties of aromatic indica rice from Bangladesh. Three crucial factors were identified for the albino shoot primordia to change into green plantlets in culture; components of M10 induction medium, callus size (range 0.2-0.4 cm long) and height of shoot primordia (range 2-3 mm). Immediate transfer of shoot primordia (2-3 mm) from M10 medium to regeneration medium followed by continuous incubation under fluorescent light (100-lux, 25±1°C) triggered albino shoot primordia to turn green in 2-3 days. Callus size did not show any effect on the change. Albino plantlets derived from anther callus cultured in KA, KB, KC, KD and KE media did not recover in both the varieties. Transfer of albino shoot primordia shorter or longer than 2-3 mm from the above 5 cultures to regeneration medium did not cause the shoot primordia to turn green. 100% albino shoot primordia initiated from Hbj B VI and 79% from Hbj B IV in M10 medium changed to green plantlets upon transfer to regeneration medium. Subsequent culture and subculture of green plantlets showed rapid formation of many new green plantlets.
authors.affiliation: Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Santosh, Tangail 1902, Bangladesh.
authors.forename: Abul Kashem Md
authors.initials: AK
authors.lastname: Mohiuddin
authors.affiliation: Biotechnology Division, Bangladesh Rice Research Institute, Gazipur 1701, Bangladesh.
authors.forename: Nilufer Hye
authors.initials: NH
authors.lastname: Karim
authors.affiliation: Biotechnology Division, Bangladesh Rice Research Institute, Gazipur 1701, Bangladesh.
authors.forename: Shahanaz
authors.initials: S
authors.lastname: Sultana
authors.affiliation: Biotechnology Division, Bangladesh Rice Research Institute, Gazipur 1701, Bangladesh.
authors.forename: Zannatul
authors.initials: Z
authors.lastname: Ferdous
keywords: Albinism; Anther Culture; Aromatic Indica Rice; Green Plant; Hobigonj Boro IV and VI