title: Cytotoxic activity and DNA-binding properties of isoeuxanthone derivatives.
abstract: In this study, the interactions of different groups substituted isoeuxanthone derivatives with calf thymus DNA (ct DNA) were investigated by spectrophotometric methods and viscosity measurements. Results indicated that the xanthone derivatives could intercalate into the DNA base pairs by the plane of xanthone ring and the various substituents may influence the binding affinity with DNA according to the calculated quenching constant values. Furthermore, two tumor cell lines including the human cervical cancer cell line (HeLa) and human hepatocellular liver carcinoma cell line (HepG2) were used to evaluate the cytotoxic activities of xanthone derivatives by acid phosphatase assay. Analyses showed that the oxiranylmethoxy substituted xanthone exhibited more effective cytotoxic activity against the cancer cells than the other substituted xanthones. The effects on the inhibition of tumor cells in vitro agreed with the studies of DNA-binding.
authors.affiliation: Key Laboratory of Interface Science and Engineering in Advanced Materials, Ministry of Education, Taiyuan University of Technology.
authors.forename: Hui Fang
authors.initials: HF
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Yan
authors.forename: Xianghua
authors.initials: X
authors.lastname: Gao
authors.forename: Baolong
authors.initials: B
authors.lastname: Niu
authors.forename: Ruijie
authors.initials: R
authors.lastname: Guo
authors.forename: Liqiao
authors.initials: L
authors.lastname: Wei
authors.forename: Bingshe
authors.initials: B
authors.lastname: Xu
authors.forename: Ning
authors.initials: N
authors.lastname: Tang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D004247:DNA / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D044004:Xanthones / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: C585166:1,6-dihydroxyxanthone; D000970:Antineoplastic Agents; D044004:Xanthones; D004247:DNA; C427203:calf thymus DNA