title: [miR-520a regulates ErbB4 expression and suppresses proliferation and invasion of esophageal squamous cell carcinoma].
abstract: OBJECTIVE
  To investigate the role of miR-520a in regulation ErbB4 expression and the biological behavior of esophageal squamous cell carcinoma (ESCC).
  
  
  METHODS
  The role of miR-520a in regulating the expression of ErbB4 was investigated by Western blotting and luciferase reporter assay system. The effect of miR-520a on the proliferation and invasion of ESCC cells was detected by MTT and Transwell invasion assay, respectively.
  
  
  RESULTS
  Western blotting and luciferase reporter assay revealed that miR-520a down-regulated the expression of ErbB4 in vitro. miR-520a significantly inhibited the proliferation and suppressed the invasion of ESCC cell line Eca109.
  
  
  CONCLUSIONS
  miR-520a regulates the expression of ErbB4 and suppresses the proliferation and invasion of ESCC cells in vitro, suggesting its role as a tumor suppressor.
authors.affiliation: Department of Gastroenterology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.E-mail: yewenguang_1982@163.com.
authors.forename: Wenguang
authors.initials: W
authors.lastname: Ye
authors.forename: Qinglin
authors.initials: Q
authors.lastname: Yao
authors.forename: Mingxin
authors.initials: M
authors.lastname: Zhang
authors.forename: Qinsheng
authors.initials: Q
authors.lastname: Wen
authors.forename: Jingjie
authors.initials: J
authors.lastname: Wang
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D004938:Esophageal Neoplasms / Q000378:metabolism* / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D066247:Receptor, ErbB-4 / Q000378:metabolism*
chemical_list: C534451:MIRN20a microRNA, human; D035683:MicroRNAs; C587689:ERBB4 protein, human; D066247:Receptor, ErbB-4