title: Metabolomic analysis of three Mollicute species.
abstract: We present a systematic study of three bacterial species that belong to the class Mollicutes, the smallest and simplest bacteria, Spiroplasma melliferum, Mycoplasma gallisepticum, and Acholeplasma laidlawii. To understand the difference in the basic principles of metabolism regulation and adaptation to environmental conditions in the three species, we analyzed the metabolome of these bacteria. Metabolic pathways were reconstructed using the proteogenomic annotation data provided by our lab. The results of metabolome, proteome and genome profiling suggest a fundamental difference in the adaptation of the three closely related Mollicute species to stress conditions. As the transaldolase is not annotated in Mollicutes, we propose variants of the pentose phosphate pathway catalyzed by annotated enzymes for three species. For metabolite detection we employed high performance liquid chromatography coupled with mass spectrometry. We used liquid chromatography method - hydrophilic interaction chromatography with silica column - as it effectively separates highly polar cellular metabolites prior to their detection by mass spectrometer.
authors.affiliation: Russian Institute of Physico-Chemical Medicine, Moscow, Russian Federation.
authors.forename: Anna A
authors.initials: AA
authors.lastname: Vanyushkina
authors.affiliation: Russian Institute of Physico-Chemical Medicine, Moscow, Russian Federation.
authors.forename: Gleb Y
authors.initials: GY
authors.lastname: Fisunov
authors.affiliation: Russian Institute of Physico-Chemical Medicine, Moscow, Russian Federation.
authors.forename: Alexey Y
authors.initials: AY
authors.lastname: Gorbachev
authors.affiliation: Russian Institute of Physico-Chemical Medicine, Moscow, Russian Federation; Russian Research Center Kurchatov Institute, Moscow, Russian Federation.
authors.forename: Dmitri E
authors.initials: DE
authors.lastname: Kamashev
authors.affiliation: Russian Institute of Physico-Chemical Medicine, Moscow, Russian Federation; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation; Moscow Institute of Physics and Technology (State University), Dolgoprudny, Moscow Region, Russian Federation.
authors.forename: Vadim M
authors.initials: VM
authors.lastname: Govorun
mesh_terms: D053898:Biosynthetic Pathways; D006867:Hydrolases / Q000378:metabolism; D013058:Mass Spectrometry; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D010427:Pentose Phosphate Pathway; D008973:Tenericutes / Q000378:metabolism*; D013729:Terpenes / Q000378:metabolism
chemical_list: D013729:Terpenes; D006867:Hydrolases; C019723:arginine deiminase