title: Investigation of circulating lncRNAs in B-cell neoplasms.
abstract: Long non-coding RNAs (lncRNA) which are longer than 200 base pairs in length, play an important role in cellular machinery. Chronic lymphocytic leukemia (CLL) and multiple myeloma (MM) are neoplasms of B-cells. In our study we aimed to investigate circulating lncRNA levels of CLL and MM patients. For this purpose we selected 5 candidate lncRNAs (TUG1, LincRNA-p21, MALAT1, HOTAIR, and GAS5) where the first two are regulated by p53. Analyses were performed by real-time PCR using cDNA synthesized from plasma RNAs. In both disease groups differential levels of plasma lncRNAs were observed. LincRNA-p21 was the only molecule displaying significant changes in the CLL group while all remaining lncRNAs showed significant differences in the MM group. In the MM group only TUG1 showed higher levels than the healthy volunteers. In conclusion, the expression levels of the candidate lncRNA molecules display a general trend for tissue- and disease-specific expression which can provide important potential biomarkers specific to the particular disease type. However, further studies are necessary to elucidate their involvement in disease development and progression.
authors.affiliation: Istanbul University, Oncology Institute, Department of Basic Oncology, Istanbul, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Isin
authors.affiliation: Istanbul University, Oncology Institute, Department of Basic Oncology, Istanbul, Turkey.
authors.forename: Emre
authors.initials: E
authors.lastname: Ozgur
authors.affiliation: Bezmialem Vakıf Gureba University, Faculty of Medicine, Department of Hematology, Istanbul, Turkey.
authors.forename: Guven
authors.initials: G
authors.lastname: Cetin
authors.affiliation: Istanbul University, Istanbul Faculty of Medicine, Department of Internal Medicine, Istanbul, Turkey.
authors.forename: Nilgun
authors.initials: N
authors.lastname: Erten
authors.affiliation: Istanbul University, Istanbul Faculty of Medicine, Department of Hematology, Istanbul, Turkey.
authors.forename: Melih
authors.initials: M
authors.lastname: Aktan
authors.affiliation: Istanbul University, Oncology Institute, Department of Basic Oncology, Istanbul, Turkey.
authors.forename: Ugur
authors.initials: U
authors.lastname: Gezer
authors.affiliation: Istanbul University, Oncology Institute, Department of Basic Oncology, Istanbul, Turkey. Electronic address: ndalay@yahoo.com.
authors.forename: Nejat
authors.initials: N
authors.lastname: Dalay
mesh_terms: D001402:B-Lymphocytes / Q000737:chemistry* / Q000378:metabolism; D017931:DNA Primers; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018450:Disease Progression; D006801:Humans; D016393:Lymphoma, B-Cell / Q000097:blood / Q000378:metabolism; D009101:Multiple Myeloma / Q000097:blood / Q000378:metabolism; D016133:Polymerase Chain Reaction; D062085:RNA, Long Noncoding / Q000032:analysis* / Q000097:blood
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D062085:RNA, Long Noncoding
keywords: Cell-free RNA; Chronic lymphocytic leukemia; Multiple myeloma; lncRNA