title: The Chlamydia trachomatis type III secretion chaperone Slc1 engages multiple early effectors, including TepP, a tyrosine-phosphorylated protein required for the recruitment of CrkI-II to nascent inclusions and innate immune signaling.
abstract: Chlamydia trachomatis, the causative agent of trachoma and sexually transmitted infections, employs a type III secretion (T3S) system to deliver effector proteins into host epithelial cells to establish a replicative vacuole. Aside from the phosphoprotein TARP, a Chlamydia effector that promotes actin re-arrangements, very few factors mediating bacterial entry and early inclusion establishment have been characterized. Like many T3S effectors, TARP requires a chaperone (Slc1) for efficient translocation into host cells. In this study, we defined proteins that associate with Slc1 in invasive C. trachomatis elementary bodies (EB) by immunoprecipitation coupled with mass spectrometry. We identified Ct875, a new Slc1 client protein and T3S effector, which we renamed TepP (Translocated early phosphoprotein). We provide evidence that T3S effectors form large molecular weight complexes with Scl1 in vitro and that Slc1 enhances their T3S-dependent secretion in a heterologous Yersinia T3S system. We demonstrate that TepP is translocated early during bacterial entry into epithelial cells and is phosphorylated at tyrosine residues by host kinases. However, TepP phosphorylation occurs later than TARP, which together with the finding that Slc1 preferentially engages TARP in EBs leads us to postulate that these effectors are translocated into the host cell at different stages during C. trachomatis invasion. TepP co-immunoprecipitated with the scaffolding proteins CrkI-II during infection and Crk was recruited to EBs at entry sites where it remained associated with nascent inclusions. Importantly, C. trachomatis mutants lacking TepP failed to recruit CrkI-II to inclusions, providing genetic confirmation of a direct role for this effector in the recruitment of a host factor. Finally, endocervical epithelial cells infected with a tepP mutant showed altered expression of a subset of genes associated with innate immune responses. We propose a model wherein TepP acts downstream of TARP to recruit scaffolding proteins at entry sites to initiate and amplify signaling cascades important for the regulation of innate immune responses to Chlamydia.
authors.affiliation: Department of Molecular Genetics and Microbiology and Center for Microbial Pathogenesis, Duke University Medical Center, Durham, North Carolina, United States of America ; Department of Biochemistry, Duke University Medical Center, Durham, North Carolina, United States of America.
authors.forename: Yi-Shan
authors.initials: YS
authors.lastname: Chen
authors.affiliation: Department of Molecular Genetics and Microbiology and Center for Microbial Pathogenesis, Duke University Medical Center, Durham, North Carolina, United States of America.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Bastidas
authors.affiliation: Department of Molecular Genetics and Microbiology and Center for Microbial Pathogenesis, Duke University Medical Center, Durham, North Carolina, United States of America.
authors.forename: Hector A
authors.initials: HA
authors.lastname: Saka
authors.affiliation: Department of Molecular Genetics and Microbiology and Center for Microbial Pathogenesis, Duke University Medical Center, Durham, North Carolina, United States of America.
authors.forename: Victoria K
authors.initials: VK
authors.lastname: Carpenter
authors.affiliation: Department of Microbiology and Immunology, University of Miami Miller School of Medicine, Miami, Florida, United States of America.
authors.forename: Kristian L
authors.initials: KL
authors.lastname: Richards
authors.affiliation: Department of Microbiology and Immunology, University of Miami Miller School of Medicine, Miami, Florida, United States of America.
authors.forename: Gregory V
authors.initials: GV
authors.lastname: Plano
authors.affiliation: Department of Molecular Genetics and Microbiology and Center for Microbial Pathogenesis, Duke University Medical Center, Durham, North Carolina, United States of America.
authors.forename: Raphael H
authors.initials: RH
authors.lastname: Valdivia
mesh_terms: D000595:Amino Acid Sequence; D002692:Chlamydia trachomatis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D002853:Chromatography, Liquid; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics* / Q000276:immunology; D047468:Immunoprecipitation; D018832:Molecular Chaperones / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D010766:Phosphorylation; D050737:Proto-Oncogene Proteins c-crk / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology; D053719:Tandem Mass Spectrometry
chemical_list: D018832:Molecular Chaperones; D050737:Proto-Oncogene Proteins c-crk