title: Automated cell-free protein production methods for structural studies.
abstract: In contrast to cell-based protein expression, cell-free production is highly consistent, scalable, and amenable to automation. Robots can handle many samples and perform repetitive procedures that are otherwise prone to human error. Here is described commercially available robotics for a wheat germ cell-free system with emphasis on practical applications for structural and functional studies. In addition, described is a cell-free method for preparing protein complexes.
authors.affiliation: Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI, 53706, USA.
authors.forename: Emily T
authors.initials: ET
authors.lastname: Beebe
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Makino
authors.forename: John L
authors.initials: JL
authors.lastname: Markley
authors.forename: Brian G
authors.initials: BG
authors.lastname: Fox
mesh_terms: D001331:Automation / Q000379:methods; D002474:Cell-Free System; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D014176:Protein Biosynthesis; D011487:Protein Conformation; D040901:Proteomics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D014908:Triticum / Q000235:genetics
chemical_list: D011994:Recombinant Proteins