title: Variability in the components of high-density lipoprotein particles measured in human ovarian follicular fluid: a cross-sectional analysis.
abstract: OBJECTIVE
  To assess the variability of follicular fluid (FF) high-density lipoprotein (HDL) particle components.
  
  
  METHODS
  Cross-sectional design with assessment using two-stage nested analysis of variance.
  
  
  METHODS
  Reproductive health center.
  
  
  METHODS
  180 in vitro fertilization (IVF) patients.
  
  
  METHODS
  None.
  
  
  METHODS
  Nineteen HDL components including HDL cholesterol and free (unesterified) and esterified forms, phospholipids, triglycerides, apolipoproteins A-1 and A-2, paraoxonase 1 (PON1) activities, and seven lipophilic vitamins and micronutrients.
  
  
  RESULTS
  We collected FF specimens from two contralateral follicles on the day of oocyte retrieval and analyzed them for HDL components, characterizing the analytes by age, body mass index (BMI), race and smoking. For some analytes, a majority of total measurement variability was attributed to sources between follicles, suggesting an important role for the integrity of the blood-follicle barrier and in situ remodeling of plasma-derived constituents. For other analytes, variability was mostly attributed to sources between women, likely indicative of plasma levels. Variability between follicles decreased with increasing age and differed by BMI and smoking, and generally were lower for Asians and women with diminished ovarian reserve.
  
  
  CONCLUSIONS
  Substantial variability in FF HDL components exist between follicles among women undergoing IVF as well as between women by age, BMI, race, smoking, and infertility diagnosis.
authors.affiliation: Department of Environmental Health Sciences, University at Albany, State University of New York, Rensselaer, New York; Department of Epidemiology and Biostatistics, University at Albany, State University of New York, Rensselaer, New York. Electronic address: mbloom@albany.edu.
authors.forename: Michael S
authors.initials: MS
authors.lastname: Bloom
authors.affiliation: Department of Environmental Health Sciences, University at Albany, State University of New York, Rensselaer, New York.
authors.forename: Keewan
authors.initials: K
authors.lastname: Kim
authors.affiliation: Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Francisco, California.
authors.forename: Victor Y
authors.initials: VY
authors.lastname: Fujimoto
authors.affiliation: Department of Biotechnical and Clinical Laboratory Sciences, University at Buffalo, State University of New York, Buffalo, New York.
authors.forename: Richard W
authors.initials: RW
authors.lastname: Browne
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D015331:Cohort Studies; D003430:Cross-Sectional Studies; D005260:Female; D005307:Fertilization in Vitro / Q000592:standards; D015571:Follicular Fluid / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008075:Lipoproteins, HDL / Q000737:chemistry / Q000378:metabolism*; D054315:Oocyte Retrieval / Q000592:standards*; D006080:Ovarian Follicle / Q000737:chemistry / Q000378:metabolism
chemical_list: D015415:Biomarkers; D008075:Lipoproteins, HDL
keywords: Biological variability; follicular fluid (FF); high density lipoprotein (HDL); infertility; in vitro fertilization (IVF)