title: Isolation and characterization of a wheat--Psathyrostachys huashanica 'Keng' 3Ns disomic addition line with resistance to stripe rust.
abstract: We isolated a wheat germplasm line, 22-2, which was derived from common wheat (Triticum aestivum '7182') and Psathyrostachys huashanica 'Keng' (2n = 2x = 14, NsNs). Genomic composition and homoeologous relationships of 22-2 was analyzed using cytology, genomic in situ hybridization (GISH), EST-SSR, and EST-STS to characterize the alien chromatin in the transfer line. The cytological investigations showed that the chromosome number and configuration were 2n = 44 = 22 II. Mitotic and meiotic GISH using P. huashanica genomic DNA as the probe indicated that 22-2 contained a pair of P. huashanica chromosomes. The genomic affinities of the introduced P. huashanica chromosomes were determined by EST-SSR and EST-STS using multiple-loci markers from seven wheat homoeologous groups between the parents and addition line. One EST-SSR and 17 EST-STS markers, which were located on the homoeologous group 3 chromosomes of wheat, amplified polymorphic bands in 22-2 that were unique to P. huashanica. Thus, these markers suggested that the introduced Ns chromosome pair belonged to homoeologous group 3, so we designated 22-2 as a 3Ns disomic addition line. Based on disease reaction to mixed races (CYR31, CYR32, and Shuiyuan14) of stripe rust in the adult stages, 22-2 was found to have high resistance to stripe rust, which was possibly derived from its P. huashanica parent. Consequently, the new disomic addition line 22-2 could be a valuable donor source for wheat improvement depending on the excellent agronomic traits, especially, the introduction of novel disease resistance genes into wheat during breeding programs.
authors.affiliation: Shaanxi Key Laboratory of Genetic Engineering for Plant Breeding, College of Agronomy, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Wanli
authors.initials: W
authors.lastname: Du
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Pang
authors.forename: Liangming
authors.initials: L
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.forename: Jixin
authors.initials: J
authors.lastname: Zhao
authors.forename: Qunhui
authors.initials: Q
authors.lastname: Yang
authors.forename: Xinhong
authors.initials: X
authors.lastname: Chen
mesh_terms: D001487:Basidiomycota / Q000254:growth & development*; D002843:Chromatin / Q000235:genetics*; D023902:Chromosome Pairing; D032461:Chromosomes, Plant; D020732:Cytogenetic Analysis; D060467:Disease Resistance; D005819:Genetic Markers; D006824:Hybridization, Genetic; D010935:Plant Diseases / Q000235:genetics*; D006109:Poaceae / Q000235:genetics* / Q000254:growth & development / Q000382:microbiology; D014908:Triticum / Q000235:genetics* / Q000254:growth & development / Q000382:microbiology; D024182:Uniparental Disomy
chemical_list: D002843:Chromatin; D005819:Genetic Markers