title: Selective evaluation of high density lipoprotein from mouse small intestine by an in situ perfusion technique.
abstract: The small intestine (SI) is the second-greatest source of HDL in mice. However, the selective evaluation of SI-derived HDL (SI-HDL) has been difficult because even the origin of HDL obtained in vivo from the intestinal lymph duct of anesthetized rodents is doubtful. To shed light on this question, we have developed a novel in situ perfusion technique using surgically isolated mouse SI, with which the possible filtration of plasma HDL into the SI lymph duct can be prevented. With the developed method, we studied the characteristics of and mechanism for the production and regulation of SI-HDL. Nascent HDL particles were detected in SI lymph perfusates in WT mice, but not in ABCA1 KO mice. SI-HDL had a high protein content and was smaller than plasma HDL. SI-HDL was rich in TG and apo AIV compared with HDL in liver perfusates. SI-HDL was increased by high-fat diets and reduced in apo E KO mice. In conclusion, with our in situ perfusion model that enables the selective evaluation of SI-HDL, we demonstrated that ABCA1 plays an important role in intestinal HDL production, and SI-HDL is small, dense, rich in apo AIV, and regulated by nutritional and genetic factors.
authors.affiliation: Laboratory of Cardiovascular Disease, Novel, Non-invasive and Nutritional Therapeutics (CNT), Graduate School of Medicine, Osaka University, Osaka, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yamaguchi
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tomonaga
authors.forename: Utako
authors.initials: U
authors.lastname: Seino
authors.forename: Akiko
authors.initials: A
authors.lastname: Kanagawa
authors.forename: Masaru
authors.initials: M
authors.lastname: Segawa
authors.forename: Hironori
authors.initials: H
authors.lastname: Nagasaka
authors.forename: Akira
authors.initials: A
authors.lastname: Suzuki
authors.forename: Takashi
authors.initials: T
authors.lastname: Miida
authors.forename: Sohsuke
authors.initials: S
authors.lastname: Yamada
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Sasaguri
authors.forename: Takefumi
authors.initials: T
authors.lastname: Doi
authors.forename: Keijiro
authors.initials: K
authors.lastname: Saku
authors.forename: Mitsuyo
authors.initials: M
authors.lastname: Okazaki
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Tochino
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Hirano
mesh_terms: D064286:ATP Binding Cassette Transporter 1 / Q000378:metabolism; D000818:Animals; D001012:Aorta, Abdominal / Q000378:metabolism; D001057:Apolipoproteins E / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D055786:Gene Knockout Techniques; D066298:In Vitro Techniques; D007421:Intestine, Small / Q000098:blood supply / Q000378:metabolism*; D008075:Lipoproteins, HDL / Q000096:biosynthesis / Q000378:metabolism*; D042601:Lymphatic Vessels / Q000378:metabolism; D008297:Male; D051379:Mice; D010446:Peptide Fragments / Q000378:metabolism; D010477:Perfusion / Q000379:methods*
chemical_list: D064286:ATP Binding Cassette Transporter 1; D001057:Apolipoproteins E; D008075:Lipoproteins, HDL; D010446:Peptide Fragments
keywords: atherosclerosis; in situ perfusion; intestine; lipoprotein; regulation