title: Parecoxib reduces systemic inflammation and acute lung injury in burned animals with delayed fluid resuscitation.
abstract: Burn injuries result in the release of proinflammatory mediators causing both local and systemic inflammation. Multiple organ dysfunctions secondary to systemic inflammation after severe burn contribute to adverse outcome, with the lungs being the first organ to fail. In this study, we evaluate the anti-inflammatory effects of Parecoxib, a parenteral COX-2 inhibitor, in a delayed fluid resuscitation burned rat model. Anaesthetized Sprague Dawley rats were inflicted with 45% total body surface area full-thickness scald burns and subsequently subjected to delayed resuscitation with Hartmann's solution. Parecoxib (0.1, 1.0, and 10 mg/kg) was delivered intramuscularly 20 min after injury followed by 12 h interval and the rats were sacrificed at 6 h, 24 h, and 48 h. Burn rats developed elevated blood cytokines, transaminase, creatinine, and increased lung MPO levels. Animals treated with 1 mg/kg Parecoxib showed significantly reduced plasma level of CINC-1, IL-6, PGEM, and lung MPO. Treatment of 1 mg/kg Parecoxib is shown to mitigate systemic and lung inflammation without significantly affecting other organs. At present, no specific therapeutic agent is available to attenuate the systemic inflammatory response secondary to burn injury. The results suggest that Parecoxib may have the potential to be used both as an analgesic and ameliorate the effects of lung injury following burn.
authors.affiliation: Naval Medical Service, Singapore Armed Forces, Singapore.
authors.forename: Si Jack
authors.initials: SJ
authors.lastname: Chong
authors.affiliation: Combat Protection and Performance Lab, Defence Medical & Environmental Research Institute, DSO National Laboratories (Kent Ridge), 27 Medical Drive No. 12-01, Singapore 117510.
authors.forename: Yong Chiat
authors.identifier: 0000-0003-4864-8490
authors.initials: YC
authors.lastname: Wong
authors.affiliation: Combat Protection and Performance Lab, Defence Medical & Environmental Research Institute, DSO National Laboratories (Kent Ridge), 27 Medical Drive No. 12-01, Singapore 117510.
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.affiliation: Combat Protection and Performance Lab, Defence Medical & Environmental Research Institute, DSO National Laboratories (Kent Ridge), 27 Medical Drive No. 12-01, Singapore 117510.
authors.forename: Mui Hong
authors.identifier: 0000-0002-2564-3112
authors.initials: MH
authors.lastname: Tan
authors.affiliation: Combat Protection and Performance Lab, Defence Medical & Environmental Research Institute, DSO National Laboratories (Kent Ridge), 27 Medical Drive No. 12-01, Singapore 117510.
authors.forename: Jia
authors.initials: J
authors.lastname: Lu
authors.affiliation: Combat Protection and Performance Lab, Defence Medical & Environmental Research Institute, DSO National Laboratories (Kent Ridge), 27 Medical Drive No. 12-01, Singapore 117510.
authors.forename: Shabbir M
authors.initials: SM
authors.lastname: Moochhala