title: Paraoxonase lactonase activity, inflammation and antioxidant status in plasma of patients with type 1 diabetes mellitus.
abstract: OBJECTIVE
  To investigate paraoxonase-1 (PON1) lactonase activity, myeloperoxidase (MPO) activity (as a marker of inflammation) and antioxidant status in plasma of patients with type 1 diabetes mellitus.
  
  
  METHODS
  Whole blood and plasma samples were collected from patients with diabetes and healthy control subjects. PON1 lactonase and MPO activities and total antioxidant capacity (TEAC) were determined in plasma. Glycosylated haemoglobin (HbA1c) was quantified in whole blood.
  
  
  RESULTS
  Plasma PON1 lactonase and MPO activities were significantly higher and TEAC was significantly lower in patients with diabetes (n = 18) compared with healthy control subjects (n = 20). There were significant positive correlations between PON1 lactonase activity and MPO activity and HbA1c level, and plasma MPO and HbA1c. There were significant negative correlations between PON1 lactonase activity and TEAC, and MPO activity and TEAC.
  
  
  CONCLUSIONS
  Increased lactonase activity may inefficiently compensate for the high level of chronic inflammation and low antioxidant capacity in the plasma of patients with type 1 diabetes mellitus.
authors.affiliation: N.C. Paulescu National Institute for Diabetes, Nutrition and Metabolic Diseases, Bucharest, Romania.
authors.forename: Octavian
authors.initials: O
authors.lastname: Savu
authors.forename: Cristian
authors.initials: C
authors.lastname: Serafinceanu
authors.forename: Ioana Veronica
authors.initials: IV
authors.lastname: Grajdeanu
authors.forename: Liviu
authors.initials: L
authors.lastname: Iosif
authors.forename: Laura
authors.initials: L
authors.lastname: Gaman
authors.forename: Irina
authors.initials: I
authors.lastname: Stoian
mesh_terms: D000293:Adolescent; D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D043303:Aryldialkylphosphatase / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D005260:Female; D006442:Glycated Hemoglobin / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000097:blood; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000097:blood*; D055815:Young Adult
chemical_list: D000975:Antioxidants; D043303:Aryldialkylphosphatase; D015415:Biomarkers; D006442:Glycated Hemoglobin; D009195:Peroxidase; C517652:hemoglobin A1c protein, human
keywords: Lactonase; antioxidants; myeloperoxidase; paraoxonase; type 1 diabetes