title: Addition of cholesterol-loaded cyclodextrins to the thawing extender: effects on boar sperm quality.
abstract: The aim of the present study was to evaluate the effect that the addition of cholesterol-loaded cyclodextrins (CLC) to the thawing extender has on the quality of frozen-thawed boar sperm. Pooled semen (n = 5) from three boars was used for the experiments. The semen was cryopreserved with an egg-yolk-based extender, it was diluted after thawing in Beltsville thawing solution (BTS) supplemented with different concentrations of CLC (0, 12.5, 25, 50 or 100 mg/500 × 10(6) sperm), and these samples were incubated at 37°C for 150 min. The following parameters of sperm quality were evaluated 30 and 150 min after incubation: sperm with intact plasma membrane (SIPM; %), sperm with normal acrosomal ridge (NAR; %), total motile sperm (TMS; %), progressively motile sperm (PMS; %) and kinetic parameters. Both SIPM and NAR increased (p < 0.05) when the thawing extender was supplemented with 12.5, 25 and 50 mg CLC/500 × 10(6) sperm. Nevertheless, motility decreased (p < 0.05) when the concentration of CLC exceeded 12.5 mg CLC/500 × 10(6) sperm. In conclusion, our results suggest that the supplementation of thawing extenders with CLC improves sperm viability and reduces acrosome damage after freezing/thawing.
authors.affiliation: Centro de Investigación y Tecnología Animal, Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA), Segorbe, Castellón, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Tomás
authors.forename: J
authors.initials: J
authors.lastname: Gómez-Fernández
authors.forename: E
authors.initials: E
authors.lastname: Gómez-Izquierdo
authors.forename: E
authors.initials: E
authors.lastname: Mocé
authors.forename: E
authors.initials: E
authors.lastname: de Mercado
mesh_terms: D000177:Acrosome / Q000187:drug effects; D000818:Animals; D002784:Cholesterol / Q000008:administration & dosage*; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary; D003451:Cryoprotective Agents / Q000008:administration & dosage*; D003505:Cyclodextrins / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D004530:Egg Yolk; D006358:Hot Temperature; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013030:Spain; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology*; D034421:Sus scrofa
chemical_list: D003451:Cryoprotective Agents; D003505:Cyclodextrins; D002784:Cholesterol