title: Specificity of Fur binding to the oxidative stress response gene promoter in the facultative anaerobic archaeon Thermoplasma volcanium.
abstract: The genome of the facultative anaerobic thermoacidophilic archaeon Thermoplasma volcanium contains the open-reading frames (ORFs) tvsod and tvogg, which are predicted to encode a putative superoxide dismutase and an 8-oxoguanine DNA glycosylase, respectively. Tvsod is immediately upstream of tvogg, and these two ORFs are aligned in a head-to-tail manner in a single operon. A previous study showed that T. volcanium contains an ORF (TVN0292) encoding the ferric uptake regulator (Fur) and that the T. volcanium Fur protein (TvFur) binds to its own promoter in a metal-dependent manner in vitro. Here, we demonstrated that TvFur also binds to the tvsod-tvogg promoter and determined the TvFur-binding sequences in the tvsod-tvogg promoter by DNaseI footprinting analysis. These results suggest that Fur is required for resistance against reactive oxygen species in this facultative anaerobic archaeon.
authors.affiliation: Department of Molecular Biology, Yokohama College of Pharmacy.
authors.forename: Hikari
authors.initials: H
authors.lastname: Minoshima
authors.forename: Yu
authors.initials: Y
authors.lastname: Ikeda
authors.forename: Miki
authors.initials: M
authors.lastname: Fujii
authors.forename: Mai
authors.initials: M
authors.lastname: Satoh
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Youichi
authors.initials: Y
authors.lastname: Itoh
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Kawashima-Ohya
authors.forename: Koji
authors.initials: K
authors.lastname: Tomobe
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Omata
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Kawashima
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009876:Operon; D018384:Oxidative Stress / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D013822:Thermoplasma / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D012097:Repressor Proteins; C068477:ferric uptake regulating proteins, bacterial