title: MUC4 modulates human glioblastoma cell proliferation and invasion by upregulating EGFR expression.
abstract: Glioblastoma (GBM), the most common primary brain tumor, is the leading cause of deaths related to tumors in the central nervous system. The prognosis of GBM patients is currently poor, and the mechanisms underlying GBM genesis remain unclear. The expression of MUC4, a high-molecular-weight and highly glycosylated protein, has been studied in many cancers. However, information on MUC4 expression in GBM is limited. In this study, we found that MUC4 was overexpressed in GBM cell lines and tissues. The proliferation and invasive potential of GBM cells were significantly increased by the ectopic expression of MUC4. By contrast, RNA interference targeting MUC4 in GBM cells significantly decreased the proliferation and invasive potential of GBM cells. We also found that the expression of epidermal growth factor receptor (EGFR) was modulated by MUC4. EGFR inhibition by siRNA reversed the MUC4-induced proliferation and invasion. These results indicated that MUC4 expression in GBM was important in GBM cell proliferation and invasion, which may be partly associated with EGFR overexpression.
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Dalian Medical University, No. 222, Zhongshan Road, Dalian 116011, PR China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Dalian Medical University, No. 222, Zhongshan Road, Dalian 116011, PR China.
authors.forename: Chunming
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Dalian Medical University, No. 222, Zhongshan Road, Dalian 116011, PR China.
authors.forename: Yiqun
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Dalian Medical University, No. 222, Zhongshan Road, Dalian 116011, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Dong
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Dalian Medical University, No. 222, Zhongshan Road, Dalian 116011, PR China.
authors.forename: Zhenqing
authors.initials: Z
authors.lastname: Wei
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Dalian Medical University, No. 222, Zhongshan Road, Dalian 116011, PR China.
authors.forename: Xiupeng
authors.initials: X
authors.lastname: Lv
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Dalian Medical University, No. 222, Zhongshan Road, Dalian 116011, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Dalian Medical University, No. 222, Zhongshan Road, Dalian 116011, PR China. Electronic address: YinghuiXu2013@126.com.
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Xu
mesh_terms: D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D002465:Cell Movement; D049109:Cell Proliferation; D066246:ErbB Receptors / Q000378:metabolism*; D005909:Glioblastoma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D055264:Mucin-4 / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D015854:Up-Regulation
chemical_list: C524658:MUC4 protein, human; D055264:Mucin-4; C512478:EGFR protein, human; D066246:ErbB Receptors
keywords: EGFR; GBM; Invasion; MUC4; Proliferation