title: Serum dipeptidyl peptidase-IV: a better screening test for early detection of mucopolysaccharidosis?
abstract: We aimed to investigate the diagnostic utility of serum DPP-IV enzyme activity, urinary GAG/Cre ratio, chitotriosidase activity, total adenosine deaminase (ADA) and ADA-1 isoenzyme activity in the diagnosis of MPS. 31 MPS patients which were previously diagnosed by clinical and enzymatic analysis and 31 healthy controls matched with age and gender were included in this study. Serum DPP-IV enzyme activity, urinary GAG/Cre ratio, total ADA and ADA-1 isoenzyme activity were significantly higher in patients than in controls (p<0.001, p<0.001, p=0.038 and p=0.006, respectively). There were significant correlations between serum DPP-IV enzyme activity and urinary GAG/Cre ratios, ADA-1 activity, ADA-1/total ADA (r=0.498, p<0.001; r=0.348, p=0.006; r=0.270, p=0.034, respectively). Area under ROC curve for DPP-IV enzyme activity was 0.988, p<0.001 and for urinary GAG/Cre ratio was 0.986, p<0.001. DPP-IV enzyme activity and urinary GAG/Cre ratio were the most significant parameters according to the univariate logistic regression analysis (p=0.001 and p<0.001, respectively). The measurement of serum DPP-IV enzyme activity can be used complementary to the urinary GAG/Cre ratio for first-line MPS screening, since it is more less prone to age and hydration related interferences.
authors.affiliation: Gülhane School of Medicine, Department of Medical Biochemistry, Ankara, Turkey.
authors.forename: Ismail
authors.initials: I
authors.lastname: Kurt
authors.affiliation: Ankara Mevki Military Hospital, Anittepe Dispensary, Biochemistry Laboratory, Ankara, Turkey. Electronic address: erdimsertoglu@gmail.com.
authors.forename: Erdim
authors.initials: E
authors.lastname: Sertoglu
authors.affiliation: Gazi University Faculty of Medicine, Department of Pediatric Metabolism and Nutrition, Ankara, Turkey.
authors.forename: Ilyas
authors.initials: I
authors.lastname: Okur
authors.affiliation: Gülhane School of Medicine, Department of Medical Biochemistry, Ankara, Turkey.
authors.forename: Serkan
authors.initials: S
authors.lastname: Tapan
authors.affiliation: Gülhane School of Medicine, Department of Medical Biochemistry, Ankara, Turkey.
authors.forename: Metin
authors.initials: M
authors.lastname: Uyanık
authors.affiliation: Adana Military Hospital, Department of Medical Biochemistry, Adana, Turkey.
authors.forename: Huseyin
authors.initials: H
authors.lastname: Kayadıbı
authors.affiliation: Gazi University Faculty of Medicine, Department of Pediatric Metabolism and Nutrition, Ankara, Turkey.
authors.forename: Fatih Suheyl
authors.initials: FS
authors.lastname: Ezgu
authors.affiliation: Gülhane School of Medicine, Department of Pediatrics, Ankara, Turkey.
authors.forename: Halil Ibrahim
authors.initials: HI
authors.lastname: Aydın
authors.affiliation: Gazi University Faculty of Medicine, Department of Pediatric Metabolism and Nutrition, Ankara, Turkey.
authors.forename: Alev
authors.initials: A
authors.lastname: Hasanoglu
mesh_terms: D000243:Adenosine Deaminase / Q000097:blood; D000293:Adolescent; D002648:Child; D003404:Creatinine / Q000652:urine; D018819:Dipeptidyl Peptidase 4 / Q000097:blood*; D042241:Early Diagnosis; D005260:Female; D006025:Glycosaminoglycans / Q000652:urine; D006596:Hexosaminidases / Q000097:blood; D006801:Humans; D007527:Isoenzymes / Q000097:blood; D008297:Male; D009083:Mucopolysaccharidoses / Q000097:blood* / Q000175:diagnosis* / Q000201:enzymology; D015203:Reproducibility of Results
chemical_list: D006025:Glycosaminoglycans; D007527:Isoenzymes; D003404:Creatinine; D006596:Hexosaminidases; C086400:chitotriosidase; D018819:Dipeptidyl Peptidase 4; D000243:Adenosine Deaminase
keywords: Mucopolysaccharidoses; Serum DPP-IV enzyme activity; Urinary GAG/Creatinine ratio