title: VelC positively controls sexual development in Aspergillus nidulans.
abstract: Fungal development and secondary metabolism is intimately associated via activities of the fungi-specific velvet family proteins including VeA, VosA, VelB and VelC. Among these, VelC has not been characterized in Aspergillus nidulans. In this study, we characterize the role of VelC in asexual and sexual development in A. nidulans. The velC mRNA specifically accumulates during the early phase of sexual development. The deletion of velC leads to increased number of conidia and reduced production of sexual fruiting bodies (cleistothecia). In the velC deletion mutant, mRNA levels of the brlA, abaA, wetA and vosA genes that control sequential activation of asexual sporulation increase. Overexpression of velC causes increased formation of cleistothecia. These results suggest that VelC functions as a positive regulator of sexual development. VelC is one of the five proteins that physically interact with VosA in yeast two-hybrid and GST pull down analyses. The ΔvelC ΔvosA double mutant produced fewer cleistothecia and behaved similar to the ΔvosA mutant, suggesting that VosA is epistatic to VelC in sexual development, and that VelC might mediate control of sex through interacting with VosA at specific life stages for sexual fruiting.
authors.affiliation: Department of Bacteriology, University of Wisconsin, Madison, Wisconsin, United States of America.
authors.forename: Hee-Soo
authors.initials: HS
authors.lastname: Park
authors.affiliation: Department of Bacteriology, University of Wisconsin, Madison, Wisconsin, United States of America.
authors.forename: Tae-Young
authors.initials: TY
authors.lastname: Nam
authors.affiliation: Department of Bacteriology, University of Wisconsin, Madison, Wisconsin, United States of America ; Department of Pharmaceutical Engineering, Woosuk University, Wanju, Republic of Korea.
authors.forename: Kap-Hoon
authors.initials: KH
authors.lastname: Han
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Dae-Jon, Republic of Korea.
authors.forename: Sun Chang
authors.initials: SC
authors.lastname: Kim
authors.affiliation: Department of Bacteriology, University of Wisconsin, Madison, Wisconsin, United States of America ; Department of Genetics, University of Wisconsin, Madison, Wisconsin, United States of America.
authors.forename: Jae-Hyuk
authors.initials: JH
authors.lastname: Yu
mesh_terms: D001233:Aspergillus nidulans / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal / Q000235:genetics / Q000502:physiology
chemical_list: D005656:Fungal Proteins