title: Enhancing the separation performance of the first-generation silica monolith using active flow technology: parallel segmented flow mode of operation.
abstract: Active flow technology (AFT) columns are designed to minimise inefficient flow processes associated with the column wall and radial heterogeneity of the stationary phase bed. This study is the first to investigate AFT on an analytical scale 4.6mm internal diameter first-generation silica monolith. The performance was compared to a conventional first-generation silica monolith and it was observed that the AFT monolith had an increase in efficiency values that ranged from 15 to 111%; the trend demonstrating efficiency gains increasing as the volumetric flow to the detector was decreased, but with no loss in sensitivity.
authors.affiliation: Australian Centre for Research on Separation Science (ACROSS), School of Science and Health, University of Western Sydney, Parramatta, NSW, Australia.
authors.forename: Arianne
authors.initials: A
authors.lastname: Soliven
authors.affiliation: Thermo Fisher Scientific, Manor Park, Tudor Road, Runcorn, UK.
authors.forename: Dominic
authors.initials: D
authors.lastname: Foley
authors.affiliation: Thermo Fisher Scientific, Manor Park, Tudor Road, Runcorn, UK.
authors.forename: Luisa
authors.initials: L
authors.lastname: Pereira
authors.affiliation: Australian Centre for Research on Separation Science (ACROSS), School of Science and Health, University of Western Sydney, Parramatta, NSW, Australia.
authors.forename: Gary R
authors.initials: GR
authors.lastname: Dennis
authors.affiliation: Australian Centre for Research on Separation Science (ACROSS), School of Science and Health, University of Western Sydney, Parramatta, NSW, Australia.
authors.forename: R Andrew
authors.initials: RA
authors.lastname: Shalliker
authors.affiliation: Research and Development, Biochemistry and Separation, Merck KGaA, Darmstadt, Germany.
authors.forename: Karin
authors.initials: K
authors.lastname: Cabrera
authors.affiliation: Thermo Fisher Scientific, Manor Park, Tudor Road, Runcorn, UK.
authors.forename: Harald
authors.initials: H
authors.lastname: Ritchie
authors.affiliation: Thermo Fisher Scientific, Manor Park, Tudor Road, Runcorn, UK. Electronic address: tony.edge@thermo.com.
authors.forename: Tony
authors.initials: T
authors.lastname: Edge
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D012822:Silicon Dioxide
keywords: Active flow technology; Curtain flow; Monolith; Parallel segmented flow; Radial heterogeneity; Silica monolith