title: Roseomonas alkaliterrae sp. nov., isolated from an alkali geothermal soil sample in Tengchong, Yunnan, South-West China.
abstract: An alkalitolerant, thermotolerant and Gram-stain negative bacterium, designated strain YIM 78007(T), was isolated from an alkaline geothermal soil sample from Hehua hot spring, Tengchong, Yunnan province, south-west China. Cells of strain YIM 78007(T) were observed to be aerobic and short rod-shaped. The colonies were observed to be orange-red, convex and circular. 16S rRNA gene sequence-based phylogenetic analysis showed that strain YIM 78007(T) clustered with members of the genus Roseomonas (with similarities from 97.2 to 92.2 %). Optimal growth of strain YIM 78007 occurs at 40-50 °C and pH 8.0-10.0. The predominant ubiquinone was identified as Q-10 and the major fatty acids were identified as C18:1 ω7c and C16:0. The polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, two unidentified aminolipids and one unknown phospholipid. The G + C content of the genomic DNA was determined to be 63 mol %. The levels of DNA-DNA hybridization relatedness between strain YIM 78007(T) and its closet neighbours (Roseomonas lacus JCM 13283(T) and Roseomonas terrae JCM 14592(T)) were well below the threshold required for the proposal of a novel species. The results of physiological and biochemical characteristics, the phylogenetic analysis, as well as low DNA-DNA hybridization values, allowed the phenotypic and genotypic differentiation of strain YIM 78007(T) from its closest phylogenetic neighbours. Therefore, strain YIM 78007(T) is considered to represent a novel species of the genus Roseomonas, for which the name Roseomonas alkaliterrae sp. nov. is proposed. The type strain is YIM 78007(T) (=BCRC 80644(T) = JCM 19656(T)).
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Dong
authors.forename: Hong
authors.initials: H
authors.lastname: Ming
authors.forename: Yi-Rui
authors.initials: YR
authors.lastname: Yin
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Duan
authors.forename: En-Min
authors.initials: EM
authors.lastname: Zhou
authors.forename: Guo-Xing
authors.initials: GX
authors.lastname: Nie
authors.forename: Hui-Geng
authors.initials: HG
authors.lastname: Feng
authors.forename: Lan
authors.initials: L
authors.lastname: Liu
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D045482:Hot Springs; D042082:Methylobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S