title: A disposable alkaline phosphatase-based biosensor for vanadium chronoamperometric determination.
abstract: A chronoamperometric method for vanadium ion determination, based on the inhibition of the enzyme alkaline phosphatase, is reported. Screen-printed carbon electrodes modified with gold nanoparticles were used as transducers for the immobilization of the enzyme. The enzymatic activity over 4-nitrophenyl phosphate sodium salt is affected by vanadium ions, which results in a decrease in the chronoamperometric current registered. The developed method has a detection limit of 0.39 ± 0.06 µM, a repeatability of 7.7% (n = 4) and a reproducibility of 8% (n = 3). A study of the possible interferences shows that the presence of Mo(VI), Cr(III), Ca(II) and W(VI), may affect vanadium determination at concentration higher than 1.0 mM. The method was successfully applied to the determination of vanadium in spiked tap water.
authors.affiliation: CELEQ and School of Chemistry, University of Costa Rica, San Pedro de Montes de Oca, San José P.O. Box 11500-2060, Costa Rica. ana.alvaradogamez@ucr.ac.cr.
authors.forename: Ana Lorena
authors.initials: AL
authors.lastname: Alvarado-Gámez
authors.affiliation: Department of Chemistry, Faculty of Sciences, University of Burgos, Plaza Misael Bañuelos s/n, Burgos 09001, Spain. malomillo@ubu.es.
authors.forename: María Asunción
authors.initials: MA
authors.lastname: Alonso-Lomillo
authors.affiliation: Department of Chemistry, Faculty of Sciences, University of Burgos, Plaza Misael Bañuelos s/n, Burgos 09001, Spain. olgado@ubu.es.
authors.forename: Olga
authors.initials: O
authors.lastname: Domínguez-Renedo
authors.affiliation: Department of Chemistry, Faculty of Sciences, University of Burgos, Plaza Misael Bañuelos s/n, Burgos 09001, Spain. jarcos@ubu.es.
authors.forename: María Julia
authors.initials: MJ
authors.lastname: Arcos-Martínez
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D004800:Enzymes, Immobilized; D006046:Gold; D057230:Limit of Detection; D053768:Metal Nanoparticles; D009596:Nitrophenols / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D015203:Reproducibility of Results; D014639:Vanadium / Q000032:analysis*; D014867:Water / Q000032:analysis
chemical_list: D004800:Enzymes, Immobilized; D009596:Nitrophenols; D009943:Organophosphorus Compounds; D014639:Vanadium; D014867:Water; C008644:nitrophenylphosphate; D006046:Gold; D000469:Alkaline Phosphatase