title: [Identification of proteins interacted with Bat3 using tandem affinity purification].
abstract: OBJECTIVE
  To identify the specific protein interactions involved in Bat3-mediated apoptosis.
  
  
  METHODS
  Tandem affinity purification (TAP) was utilized to investigate Bat3-protein interactions, during which full-length human Bat3 fused with Strep2 and FLAG tag as a bait was used to screen the specific protein-protein interactions. The isolated proteins were identified with mass spectrometry.
  
  
  RESULTS
  TAP studies showed that Ubl4A was identified as a Bat3-binding partner. Further investigation using co-immunoprecipitation confirmed that Bat3 was associated with Ubl4A.
  
  
  CONCLUSIONS
  TAP was successfully established and is suitable for isolating the binding partners of Bat3.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: Qin-shan
authors.initials: QS
authors.lastname: Li
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: Shi-ying
authors.initials: SY
authors.lastname: Miao
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: Lin-fang
authors.initials: LF
authors.lastname: Wang
mesh_terms: D002460:Cell Line; D006801:Humans; D018832:Molecular Chaperones / Q000302:isolation & purification*; D011485:Protein Binding; D014452:Ubiquitins / Q000302:isolation & purification*
chemical_list: C092009:BAG6 protein, human; D018832:Molecular Chaperones; D014452:Ubiquitins; C583268:Ubl4A protein, human