title: Characterization of eosinophilic esophagitis murine models using optical coherence tomography.
abstract: Pre-clinical studies using murine models are critical for understanding the pathophysiological mechanisms underlying immune-mediated disorders such as Eosinophilic esophagitis (EoE). In this study, an optical coherence tomography (OCT) system capable of providing three-dimensional images with axial and transverse resolutions of 5 µm and 10 µm, respectively, was utilized to obtain esophageal images from a murine model of EoE-like disease ex vivo. Structural changes in the esophagus of wild-type (Tslpr(+/+) ) and mutant (Tslpr(-/-) ) mice with EoE-like disease were quantitatively evaluated and food impaction sites in the esophagus of diseased mice were monitored using OCT. Here, the capability of OCT as a label-free imaging tool devoid of tissue-processing artifacts to effectively characterize murine EoE-like disease models has been demonstrated.
authors.affiliation: Department of Electrical and Computer Engineering, Lehigh University, Bethlehem, PA-18015, USA ; Center for Photonics and Nanoelectronics, Lehigh University, Bethlehem, PA-18015, USA.
authors.forename: Aneesh
authors.initials: A
authors.lastname: Alex
authors.affiliation: Department of Microbiology University of Pennsylvania, Philadelphia, PA 19104, USA ; Institute for Immunology, Perelman School of Medicine University of Pennsylvania, Philadelphia, PA 19104, USA ; Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: Mario
authors.initials: M
authors.lastname: Noti
authors.affiliation: Department of Microbiology University of Pennsylvania, Philadelphia, PA 19104, USA ; Institute for Immunology, Perelman School of Medicine University of Pennsylvania, Philadelphia, PA 19104, USA ; Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: Elia D Tait
authors.initials: ED
authors.lastname: Wojno
authors.affiliation: Department of Microbiology University of Pennsylvania, Philadelphia, PA 19104, USA ; Institute for Immunology, Perelman School of Medicine University of Pennsylvania, Philadelphia, PA 19104, USA ; Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: David
authors.initials: D
authors.lastname: Artis
authors.affiliation: Department of Electrical and Computer Engineering, Lehigh University, Bethlehem, PA-18015, USA ; Center for Photonics and Nanoelectronics, Lehigh University, Bethlehem, PA-18015, USA ; Bioengineering Program, Lehigh University, Bethlehem, PA-18015, USA.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhou
keywords: (110.4500) Optical coherence tomography; (110.6880) Three-dimensional image acquisition; (170.0110) Imaging systems; (170.2680) Gastrointestinal; (170.3880) Medical and biological imaging; (170.6935) Tissue characterization