title: Genistein-inhibited cancer stem cell-like properties and reduced chemoresistance of gastric cancer.
abstract: Genistein, the predominant isoflavone found in soy products, has exerted its anticarcinogenic effect in many different tumor types in vitro and in vivo. Accumulating evidence in recent years has strongly indicated the existence of cancer stem cells in gastric cancer. Here, we showed that low doses of genistein (15 µM), extracted from Millettia nitida Benth var hirsutissima Z Wei, inhibit tumor cell self-renewal in two types of gastric cancer cells by colony formation assay and tumor sphere formation assay. Treatment of gastric cancer cells with genistein reduced its chemoresistance to 5-Fu (fluorouracil) and ciplatin. Further results indicated that the reduced chemoresistance may be associated with the inhibition of ABCG2 expression and ERK 1/2 activity. Furthermore, genistein reduced tumor mass in the xenograft model. Together, genistein inhibited gastric cancer stem cell-like properties and reduced its chemoresistance. Our results provide a further rationale and experimental basis for using the genistein to improve treatment of patients with gastric cancer.
authors.affiliation: Department of Surgical Oncology, First Affiliated Hospital of Xiamen University & Xiamen Cancer Center, Xiamen 361003, Fujian, China. huangwf006@163.com.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Huang
authors.affiliation: Jiangxi Key Laboratory for Postharvest Technology and Nondestructive Testing of Fruits & Vegetables, College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, Jiangxi, China. lemonwan@126.com.
authors.forename: Chunpeng
authors.initials: C
authors.lastname: Wan
authors.affiliation: Department of Surgical Oncology, First Affiliated Hospital of Xiamen University & Xiamen Cancer Center, Xiamen 361003, Fujian, China. qcluo@xmu.edu.cn.
authors.forename: Qicong
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Department of Surgical Oncology, First Affiliated Hospital of Xiamen University & Xiamen Cancer Center, Xiamen 361003, Fujian, China. h74zj@126.com.
authors.forename: Zhengjie
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Department of Surgical Oncology, First Affiliated Hospital of Xiamen University & Xiamen Cancer Center, Xiamen 361003, Fujian, China. luoqixmzsh@126.com.
authors.forename: Qi
authors.initials: Q
authors.lastname: Luo
mesh_terms: D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D005260:Female; D005472:Fluorouracil / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D019833:Genistein / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008819:Mice, Nude; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D015394:Molecular Structure; D009363:Neoplasm Proteins / Q000235:genetics; D014411:Neoplastic Stem Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D014410:Tumor Stem Cell Assay; D023041:Xenograft Model Antitumor Assays
chemical_list: C116614:ABCG2 protein, human; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters; D000970:Antineoplastic Agents; D009363:Neoplasm Proteins; D019833:Genistein; C535150:MAPK1 protein, human; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D002945:Cisplatin; D005472:Fluorouracil