title: Lymphatic involvement in the disappearance of steroidogenic cells from the corpus luteum during luteolysis.
abstract: In mammals, the corpus luteum (CL) is an essential endocrine gland for the establishment and maintenance of pregnancy. If pregnancy is not established, the CL regresses and disappears rapidly from the ovary. A possible explanation for the rapid disappearance of the CL is that luteal cells are transported from the ovary via lymphatic vessels. Here, we report the presence of cells positive for 3β-hydroxysteroid dehydrogenase (3β-HSD), an enzyme involved in progesterone synthesis, in the lumen of lymphatic vessels at the regressing luteal stage and in the lymphatic fluid collected from the ovarian pedicle ipsilateral to the regressing CL. The 3β-HSD positive cells were alive and contained lipid droplets. The 3β-HSD positive cells in the lymphatic fluid were most abundant at days 22-24 after ovulation. These findings show that live steroidogenic cells are in the lymphatic vessels drained from the CL. The outflow of steroidogenic cells starts at the regressing luteal stage and continues after next ovulation. The overall findings suggest that the complete disappearance of the CL during luteolysis is involved in the outflow of luteal cells from the CL via ovarian lymphatic vessels.
authors.affiliation: Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan.
authors.forename: Hironori
authors.initials: H
authors.lastname: Abe
authors.affiliation: Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan.
authors.forename: Mohamad Omar
authors.initials: MO
authors.lastname: Al-zi'abi
authors.affiliation: Sekizawa Animal Clinic, Tochigi, Japan.
authors.forename: Fumio
authors.initials: F
authors.lastname: Sekizawa
authors.affiliation: Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan.
authors.forename: Tomas J
authors.initials: TJ
authors.lastname: Acosta
authors.affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.
authors.forename: Dariusz J
authors.initials: DJ
authors.lastname: Skarzynski
authors.affiliation: Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Okuda
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D002417:Cattle; D003338:Corpus Luteum / Q000166:cytology* / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism*; D005260:Female; D005453:Fluorescence; D007150:Immunohistochemistry; D003341:Luteolysis / Q000502:physiology*; D042601:Lymphatic Vessels / Q000166:cytology / Q000378:metabolism; D011247:Pregnancy
chemical_list: D015096:3-Hydroxysteroid Dehydrogenases