title: Protective effects of desferrioxamine and deferiprone on the spleen tissue of aluminum intoxicated mice: A Fourier transform infrared spectroscopy study.
abstract: The present study was designed to examine the protective effects of the chelating agents desferrioxamine (DFO) and deferiprone (DFP) in aluminum intoxicated spleen tissue of mice by Fourier transform infrared (FTIR) spectroscopy. The finding revealed the alterations on the major biochemical constituents, such as lipids, proteins, phosphodiester and nucleic acids of the spleen tissue of mice at molecular level. The significant decreased in the peak areas of asymmetric and symmetric mode of the phosphodiester groups from control to aluminum intoxicated, but improved it by DFP and DFO+DFP treatments respectively for nearer control value. The bands ratio at I1081/I1232 significantly decreased from control to aluminum, but enhanced it by DFP and DFO+DFP respectively. This result suggests that DFO and DFP are the phosphodiesterase inhibitor, recovered from chronic growth of diseases in the spleen. Amide I and amide II peak area values decreased from control to aluminum intoxicated spleen tissue, but treated with DFP and DFO+DFP significantly improved. This result suggests an alteration in the protein profile. The absence of Olefinic=CH stretching in aluminum intoxicated spleen suggests an altered lipid levels. Concentrations of trace elements were found by ICP-OES. Histopathological findings confirmed the biochemical observations of this study. The results of the FTIR study were found to be in agreement with biochemical studies and demonstrated that FTIR can be used successfully applied to toxicological studies at molecular level.
authors.affiliation: Department of Physics, Annamalai University, Annamalai Nagar, Tamilnadu 608002, India. Electronic address: girihari777@yahoo.com.
authors.forename: S
authors.initials: S
authors.lastname: Sivakumar
authors.affiliation: Department of Physics, Annamalai University, Annamalai Nagar, Tamilnadu 608002, India.
authors.forename: Chandra Prasad
authors.initials: CP
authors.lastname: Khatiwada
authors.affiliation: Department of Physics, Annamalai University, Annamalai Nagar, Tamilnadu 608002, India.
authors.forename: J
authors.initials: J
authors.lastname: Sivasubramanian
mesh_terms: D000535:Aluminum / Q000633:toxicity*; D000818:Animals; D000077543:Deferiprone; D003676:Deferoxamine / Q000494:pharmacology*; D008297:Male; D051379:Mice; D020011:Protective Agents / Q000494:pharmacology*; D011728:Pyridones / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D013154:Spleen / Q000737:chemistry / Q000187:drug effects* / Q000473:pathology*
chemical_list: D020011:Protective Agents; D011728:Pyridones; D000077543:Deferiprone; D000535:Aluminum; D003676:Deferoxamine
keywords: Aluminum; DFO and DFP; FTIR; ICP–OES; Spleen