title: Colorado potato beetle (Coleoptera) gut transcriptome analysis: expression of RNA interference-related genes.
abstract: In the search for new methods of pest control, the potential of RNA interference (RNAi) is being explored. Because the gut is the first barrier for the uptake of double-stranded (ds)RNA, pyrosequencing of the gut transcriptome is a powerful tool for obtaining the necessary sequences for specific dsRNA-mediated pest control. In the present study, a dataset representing the gut transcriptome of the Colorado potato beetle (CPB; Leptinotarsa decemlineata) was generated and analysed for the presence of RNAi-related genes. Almost all selected genes that were implicated in silencing efficiency at different levels in the RNAi pathway (core machinery, associated intracellular factors, dsRNA uptake, antiviral RNAi, nucleases), which uses different types of small RNA (small interfering RNA, microRNA and piwi-RNA), were expressed in the CPB gut. Although the database is of lower quality, the majority of the RNAi genes are also found to be present in the gut transcriptome of the tobacco hornworm [TH; Manduca sexta (19 out of 35 genes analysed)]. The high quality of the CPB transcriptome database will lay the foundation for future gene expression and functional studies regarding the gut and RNAi.
authors.affiliation: Insect Molecular Genetics and Biotechnology, NCSR 'Demokritos', Institute of Biosciences & Applications, Athens, Greece.
authors.forename: L
authors.initials: L
authors.lastname: Swevers
authors.forename: H
authors.initials: H
authors.lastname: Huvenne
authors.forename: G
authors.initials: G
authors.lastname: Menschaert
authors.forename: D
authors.initials: D
authors.lastname: Kontogiannatos
authors.forename: A
authors.initials: A
authors.lastname: Kourti
authors.forename: Y
authors.initials: Y
authors.lastname: Pauchet
authors.forename: R
authors.initials: R
authors.lastname: ffrench-Constant
authors.forename: G
authors.initials: G
authors.lastname: Smagghe
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000235:genetics*; D041981:Gastrointestinal Tract; D015870:Gene Expression; D018402:Manduca / Q000235:genetics; D010802:Phylogeny; D034622:RNA Interference; D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering; D059467:Transcriptome
chemical_list: D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering
keywords: 454 sequencing; Colorado potato beetle; Leptinotarsa decemlineata; Manduca sexta; RNA interference; RNAi machinery; dsRNA uptake; gut transcriptome; microRNA; piRNA; siRNA; tobacco hornworm