title: Development of a method for the determination of total bisphenol a at trace levels in human blood and urine and elucidation of factors influencing method accuracy and sensitivity.
abstract: This publication describes a method for the determination of total bisphenol A (BPA and conjugated BPA) following enzyme hydrolysis and is intended as a companion to our previously developed analytical method for the determination of free BPA (the aglycone) in human blood and urine using high-performance liquid chromatography-tandem mass spectrometry ( 1). That free BPA method provided a means to account for and/or eliminate background contamination and demonstrated accuracy and reproducibility in both matrices fortified with BPA or a surrogate analyte ((13)C BPA) at a low method quantitation limit (MQL) of 0.1-0.2 ng/mL. In contrast to the free BPA method results and based on stringent accuracy, precision and confirmation criteria set for the MQLs of the method developed for total BPA, the MQL achieved in blood was 1.020-2.550 and 0.510-1.020 ng/mL in urine. These data showed higher MQLs than the desired MQLs of 0.5 ng/mL (blood) and 0.2 ng/mL (urine) with increased variability between analyses which demonstrates the importance of generating method validation data with each analysis. In contrast, the MQL achieved for (13)C BPA-G (monoglucuronide as a surrogate analyte in blood was 0.2-0.5 and 0.2 ng/mL in urine illustrating that the method is capable of meeting lower MQL requirements if the contribution from exogenous BPA can be well controlled. This method for the determination total BPA in human blood and urine is intended to be used in conjunction with the free BPA method ( 1) to obtain accurate and complete BPA biomonitoring data to support human exposure assessments.
authors.affiliation: 1Toxicology and Environmental Research & Consulting, The Dow Chemical Company, Midland, MI, USA.
authors.forename: Dan
authors.initials: D
authors.lastname: Markham
authors.forename: John
authors.initials: J
authors.lastname: Waechter
authors.forename: Robert
authors.initials: R
authors.lastname: Budinsky
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Gries
authors.forename: Dieter
authors.initials: D
authors.lastname: Beyer
authors.forename: Stephanie
authors.initials: S
authors.lastname: Snyder
authors.forename: Stephen
authors.initials: S
authors.lastname: Dimond
authors.forename: V N
authors.initials: VN
authors.lastname: Rajesh
authors.forename: Narayana
authors.initials: N
authors.lastname: Rao
authors.forename: Paul
authors.initials: P
authors.lastname: Connolly
authors.forename: Mark
authors.initials: M
authors.lastname: Neeley
authors.forename: Steven
authors.initials: S
authors.lastname: Hentges
mesh_terms: D001559:Benzhydryl Compounds / Q000097:blood / Q000652:urine; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods*; D004785:Environmental Pollutants / Q000097:blood / Q000652:urine; D006801:Humans; D057230:Limit of Detection; D010636:Phenols / Q000097:blood / Q000652:urine; D012015:Reference Standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004785:Environmental Pollutants; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds