title: Placental infiltration of inflammatory markers in gestational diabetic women.
abstract: Gestational diabetes mellitus (GDM) is pathology of glucose intolerance during pregnancy. It is influenced by maternal hyperglycemia and insulinemia through placental circulation. The study was undertaken to investigate the implication of pro-inflammatory factors in the placenta of GDM women. Thirty GDM women have delivered macrosomic babies, and 30 healthy age-matched pregnant women have delivered non macrosomic babies, were recruited in the study. The mRNAs encoding for IL-6, TLR4, TGF-β, CD68, CD14, EMR-1, CCL2, TCR-α, T-bet, GATA-3, leptin and adiponectin were quantified in placental samples by using RT-qPCR. The mRNA expression of the pro-inflammatory factors, i.e., IL-6, TLR4 and TGF-β, was increased in GDM placenta. The mRNA expression of markers of infiltration of macrophage, i.e., CD68, CD14 and EMR-1, was higher in the GDM placenta than the control placenta. The expression of mRNA of TCR-α, an indicator of T-cell infiltration, was significantly higher in the GDM placenta. Interestingly, the expression of mRNA of GATA-3, an indicator of Th2 phenotype differentiation, was unregulated in the GDM placenta. Leptin and adiponectin mRNAs were also significantly increased in the placenta of the GDM group. Our results revealed that there is an increase of inflammation in the GDM placenta which might be involved, in part, in the pathogenesis of macrosomia.
authors.affiliation: University of Burgundy, INSERM U866, Faculty of Life Sciences, Dijon, France. ines.mrizak@hotmail.com.
authors.forename: Ines
authors.initials: I
authors.lastname: Mrizak
authors.forename: Oussama
authors.initials: O
authors.lastname: Grissa
authors.forename: Benoit
authors.initials: B
authors.lastname: Henault
authors.forename: Mariem
authors.initials: M
authors.lastname: Fekih
authors.forename: Ali
authors.initials: A
authors.lastname: Bouslema
authors.forename: Imen
authors.initials: I
authors.lastname: Boumaiza
authors.forename: Monia
authors.initials: M
authors.lastname: Zaouali
authors.forename: Zouhair
authors.initials: Z
authors.lastname: Tabka
authors.forename: Naim A
authors.initials: NA
authors.lastname: Khan
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D016640:Diabetes, Gestational / Q000097:blood / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006442:Glycated Hemoglobin / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000235:genetics / Q000378:metabolism; D007328:Insulin / Q000097:blood; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
chemical_list: D015415:Biomarkers; D001786:Blood Glucose; D006442:Glycated Hemoglobin; D007328:Insulin; D012333:RNA, Messenger