title: Investigation of the association between ATP2B4 and ATP5B genes with colorectal cancer.
abstract: Colorectal cancer (CRC) develops as a multi-step process which results from gradual accumulation of mutations in proto-oncogenes, tumor suppressor, and DNA repair genes. Mortality rate of CRC is very high. Therefore, development of alternative diagnostic methods which can be used in the early diagnosis is crucial. ATP2B4 gene encodes one of the four isoforms of p-type ATPase PMCA enzyme and bears critical importance in maintaining the balance of intracellular calcium homeostasis by providing the export of calcium ions out of the cell. ATP5B encodes a subunit of the mitochondrial ATP synthase which is an f-type ATPase. In this study, the relationship between ATP2B4 and ATP5B genes and CRC regarding gene expression was investigated. Study groups were constructed from a number of 50 patients (25 males, 25 females) with the mean age of 55.68 ± 9.4 and the gene expression levels in the healthy and cancerous tissues of the patients were compared by using semi-quantitative PCR and Real-Time PCR methods. As a result, in patients with rectum tumors, there was a significant relationship between ATP2B4 gene expression and the tumor location and in patients younger than 45 years, ATP5B gene expressions were detected significantly higher in tumor tissues by using RT-PCR. However, no significant relationship was detected in terms of expression differences of ATP2B4 and ATP5B genes between cancerous and healthy tissues of the CRC patients. ATP2B4 and ATP5B genes might have indirect associations in CRC pathogenesis and the investigation of their interactions with DNA repair and other related genes may help in understanding of CRC formation.
authors.affiliation: Gaziantep University, Faculty of Medicine, Department of Medical Biology, Gaziantep, Turkey. Electronic address: gyk.esra@gmail.com.
authors.forename: Esra
authors.initials: E
authors.lastname: Geyik
authors.affiliation: Gaziantep University, Faculty of Medicine, Department of Medical Biology, Gaziantep, Turkey.
authors.forename: Yusuf Ziya
authors.initials: YZ
authors.lastname: Igci
authors.affiliation: Gaziantep University, Faculty of Medicine, Department of Medical Biology, Gaziantep, Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Pala
authors.affiliation: Gaziantep University, Faculty of Medicine, Department of Medical Oncology, Gaziantep, Turkey.
authors.forename: Ali
authors.initials: A
authors.lastname: Suner
authors.affiliation: Gaziantep University, Faculty of Medicine, Department of General Surgery, Gaziantep, Turkey.
authors.forename: Ersin
authors.initials: E
authors.lastname: Borazan
authors.affiliation: Adiyaman University, Department of Medical Biology, Adiyaman, Turkey.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Bozgeyik
authors.affiliation: Gaziantep University, Faculty of Medicine, Department of Medical Biology, Gaziantep, Turkey.
authors.forename: Emine
authors.initials: E
authors.lastname: Bayraktar
authors.affiliation: Gaziantep University, Faculty of Medicine, Department of Medical Biology, Gaziantep, Turkey.
authors.forename: Recep
authors.initials: R
authors.lastname: Bayraktar
authors.affiliation: Gaziantep University, Faculty of Medicine, Department of Medical Biology, Gaziantep, Turkey.
authors.forename: Sercan
authors.initials: S
authors.lastname: Ergun
authors.affiliation: Gaziantep University, Faculty of Medicine, Department of Medical Biology, Gaziantep, Turkey.
authors.forename: Ecir Ali
authors.initials: EA
authors.lastname: Cakmak
authors.affiliation: Gaziantep University, Faculty of Medicine, Department of General Surgery, Gaziantep, Turkey.
authors.forename: Avni
authors.initials: A
authors.lastname: Gokalp
authors.affiliation: Gaziantep University, Faculty of Medicine, Department of Medical Biology, Gaziantep, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Arslan
mesh_terms: D000368:Aged; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D056726:Genetic Association Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics* / Q000378:metabolism; D053499:Plasma Membrane Calcium-Transporting ATPases / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C506526:ATP5F1B protein, human; C507517:ATP2B4 protein, human; D025261:Mitochondrial Proton-Translocating ATPases; D053499:Plasma Membrane Calcium-Transporting ATPases
keywords: ATP2B4; ATP5B; Colorectal cancer; Gene expression