title: Preparation of chondroitin sulfate-g-poly(ε-caprolactone) copolymers as a CD44-targeted vehicle for enhanced intracellular uptake.
abstract: Chondroitin sulfate-g-poly(ε-caprolactone) (CP) copolymers were synthesized via atom transfer radical addition (ATRA). The CP copolymers self-assembled into micelles in water, and the micelles could be used to encapsulate a hydrophobic anticancer drug, camptothecin (CPT), in the core for tumor targeting delivery. The physicochemical properties of the micelles and CPT-loaded micelles were thoroughly characterized. For the in vitro test, the CPT release, the protection of the lactone ring of CPT from hydrolysis and the cellular uptake of CPT were studied. The cell-killing and apoptosis-inducing effects using the CPT-loaded micelles were significantly better than using free CPT against CRL-5802 cells. The micellar internalization into CRL-5802 cells was primarily via CD44 and clathrin dual-mediated endocytosis. For the in vivo test, the therapeutic efficacy of the CPT-loaded micelles was studied in a non-small-cell lung cancer xenograft animal model. The CPT-loaded micelles showed good inhibition in tumor growth as compared with a commercial product, CPT-11, in CRL-5802 tumor-bearing mice. The in vitro and in vivo data suggested the CP-based micelles are promising anticancer drug vehicles for lung cancer targeting.
authors.affiliation: Department of Medicinal & Applied Chemistry, ‡Department of Biotechnology, and §School of Pharmacy, Kaohsiung Medical University , Kaohsiung 80708, Taiwan.
authors.forename: Yu-Sheng
authors.initials: YS
authors.lastname: Liu
authors.forename: Chien-Chih
authors.initials: CC
authors.lastname: Chiu
authors.forename: Hsuan-Ying
authors.initials: HY
authors.lastname: Chen
authors.forename: Su-Hwei
authors.initials: SH
authors.lastname: Chen
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Wang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D002166:Camptothecin / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D002809:Chondroitin Sulfates / Q000008:administration & dosage*; D016503:Drug Delivery Systems; D004355:Drug Stability; D004705:Endocytosis; D018960:Hyaluronan Receptors / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008823:Micelles; D011091:Polyesters / Q000008:administration & dosage*
chemical_list: D000970:Antineoplastic Agents; D018960:Hyaluronan Receptors; D008823:Micelles; D011091:Polyesters; C016240:polycaprolactone; D002809:Chondroitin Sulfates; D002166:Camptothecin