title: Prevalence of Bordetella holmesii and Bordetella bronchiseptica in respiratory tract samples from Belgian patients with pertussis-like symptoms by sensitive culture method and mass spectrometry.
abstract: Insertion sequences IS481 and IS1001 are targets for molecular detection of respectively Bordetella pertussis and Bordetella parapertussis. There is a raising concern about specificity of these targets due to sequence similarity with Bordetella holmesii and Bordetella bronchiseptica. The likelihood of false (para)pertussis diagnoses should be correlated with the prevalence of these organisms in the respiratory tract (RT). From October 2010 until September 2011, 2,207 RT samples were submitted to the Belgian reference laboratory for pertussis diagnosis. End-point IS481/IS 1001 PCR and culture were performed for B. pertussis and B. parapertussis. We developed a sensitive culture method followed by screening with matrix-assisted laser desorption/ionisation- time of flight mass spectrometry (MALDI-TOF MS) to look for B. holmesii and B. bronchiseptica in our samples,. Only one B. bronchiseptica and no B. holmesii were detected in RT samples from Belgian patients with pertussis-like symptoms.
authors.affiliation: National Reference Centre for Bordetella Pertussis, Department of Microbiology and Infection Control, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel (VUB), Belgium. Dorien.VandenBossche@uzbrussel.be
authors.forename: D
authors.initials: D
authors.lastname: Van den Bossche
authors.affiliation: National Reference Centre for Bordetella Pertussis, Department of Microbiology and Infection Control, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel (VUB), Belgium.
authors.forename: A
authors.initials: A
authors.lastname: De Bel
authors.affiliation: National Reference Centre for Bordetella Pertussis, Department of Microbiology and Infection Control, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel (VUB), Belgium.
authors.forename: D
authors.initials: D
authors.lastname: De Smet
authors.affiliation: National Reference Centre for Bordetella Pertussis, Department of Microbiology and Infection Control, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel (VUB), Belgium.
authors.forename: O
authors.initials: O
authors.lastname: Heylen
authors.affiliation: National Reference Centre for Bordetella Pertussis, Department of Microbiology and Infection Control, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel (VUB), Belgium.
authors.forename: E
authors.initials: E
authors.lastname: Vekens
authors.affiliation: National Reference Centre for Bordetella Pertussis, Department of Microbiology and Infection Control, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel (VUB), Belgium.
authors.forename: K
authors.initials: K
authors.lastname: Vandoorslaer
authors.affiliation: National Reference Centre for Bordetella Pertussis, Department of Microbiology and Infection Control, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel (VUB), Belgium.
authors.forename: O
authors.initials: O
authors.lastname: Soetens
authors.affiliation: National Reference Centre for Bordetella Pertussis, Department of Microbiology and Infection Control, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel (VUB), Belgium.
authors.forename: D
authors.initials: D
authors.lastname: Piérard
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001431:Bacteriological Techniques; D001530:Belgium / Q000453:epidemiology; D001884:Bordetella / Q000302:isolation & purification*; D001885:Bordetella Infections / Q000382:microbiology*; D016950:Bordetella bronchiseptica / Q000302:isolation & purification; D042483:Bordetella parapertussis / Q000302:isolation & purification; D001886:Bordetella pertussis / Q000302:isolation & purification; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007223:Infant; D013058:Mass Spectrometry; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012137:Respiratory System / Q000382:microbiology; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization