title: The effect of ovarian puncture on the endocrine profile of PCOS patients who undergo IVM.
abstract: BACKGROUND
  To examine whether ovarian puncture for immature oocyte retrieval and in-vitro maturation (IVM) has an effect on the endocrine profile of patients with polycystic ovary syndrome (PCOS).
  
  
  METHODS
  Twenty-two consecutive patients with PCOS undergoing IVM treatment were included. Serum anti-Müllerian hormone (AMH), sex hormone-binding globulin (SHBG), total testosterone (TT) and luteinized hormone (LH) levels were analyzed at the start of the cycle, on the day of immature oocyte retrieval (OR) and at fixed intervals thereafter, for up to three months after OR.
  
  
  RESULTS
  Five days after OR circulating AMH, TT, calculated free testosterone (FTc), and LH levels were significantly reduced and circulating SHBG was significantly increased. Two weeks after OR, TT, FTc and LH remained reduced, whereas circulating AMH and SHBG levels recovered to pre-puncture values. Three months after OR, all circulating hormone levels had recovered to baseline values.
  
  
  CONCLUSIONS
  Ovarian puncture for the retrieval of immature oocytes and IVM in patients with PCOS has a significant impact on the ovarian endocrine profile, but this impact is brief and transient.
authors.affiliation: Centre for Reproductive Medicine, UZ Brussel, Laarbeeklaan 101, 1090 Brussels, Belgium. cortegah@gmail.com.
authors.forename: Carolina
authors.initials: C
authors.lastname: Ortega-Hrepich
authors.forename: Nikolaos P
authors.initials: NP
authors.lastname: Polyzos
authors.forename: Ellen
authors.initials: E
authors.lastname: Anckaert
authors.forename: Luis
authors.initials: L
authors.lastname: Guzman
authors.forename: Herman
authors.initials: H
authors.lastname: Tournaye
authors.forename: Johan
authors.initials: J
authors.lastname: Smitz
authors.forename: Michel
authors.initials: M
authors.lastname: De Vos
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D015331:Cohort Studies; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D059471:In Vitro Oocyte Maturation Techniques / Q000295:instrumentation / Q000379:methods*; D007986:Luteinizing Hormone / Q000097:blood; D054315:Oocyte Retrieval / Q000295:instrumentation / Q000379:methods; D010053:Ovary / Q000378:metabolism*; D011085:Polycystic Ovary Syndrome / Q000097:blood* / Q000175:diagnosis / Q000628:therapy*; D011677:Punctures / Q000295:instrumentation / Q000379:methods*; D012738:Sex Hormone-Binding Globulin / Q000378:metabolism; D013739:Testosterone / Q000097:blood; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D015415:Biomarkers; D012738:Sex Hormone-Binding Globulin; D013739:Testosterone; D007986:Luteinizing Hormone