title: Proteomic and genomic analyses suggest the association of apolipoprotein C1 with abdominal aortic aneurysm.
abstract: OBJECTIVE
  Abdominal aortic aneurysm (AAA) is an important cause of mortality in the elderly. Mouse models are widely used to investigate AAA pathogenesis but their suitability for biomarker discovery is unexplored.
  
  
  METHODS
  We conducted a three-phase study. Phase 1: Aortas from angiotensin-II-infused apolipoprotein E deficient (ApoE(-/-) ) mice with and without AAA were assessed via iTRAQ and analyzed in silico to identify potential circulating markers. Microarray data from ApoE(-/-) mice and human patients were analyzed in parallel. Phase 2: Putative markers were compared between datasets to shortlist common candidates. Phase 3: The relationship of two shortlisted markers and AAA presence was assessed.
  
  
  RESULTS
  iTRAQ identified eight proteins with biomarker potential. Microarray data identified 72 and 96 potential biomarkers from ApoE(-/-) mice and human patients, respectively. All three datasets suggested apolipoprotein C1 (ApoC1) as a marker for AAA; microarray data identified matrix metalloproteinase 9 (MMP9) as a second potential marker. Plasma ApoC1 and MMP9 concentrations positively correlated with AAA diameter in ApoE(-/-) mice.
  
  
  CONCLUSIONS
  ApoC1 may be a novel biomarker for AAA.
authors.affiliation: Vascular Biology Unit, Queensland Research Centre for Peripheral Vascular Disease, School of Medicine and Dentistry, James Cook University, Townsville, Australia.
authors.forename: Joseph V
authors.initials: JV
authors.lastname: Moxon
authors.forename: Dawei
authors.initials: D
authors.lastname: Liu
authors.forename: Corey S
authors.initials: CS
authors.lastname: Moran
authors.forename: David J
authors.initials: DJ
authors.lastname: Crossman
authors.forename: Smriti M
authors.initials: SM
authors.lastname: Krishna
authors.forename: Ponlapat
authors.initials: P
authors.lastname: Yonglitthipagon
authors.forename: Theophilus I
authors.initials: TI
authors.lastname: Emeto
authors.forename: Dylan R
authors.initials: DR
authors.lastname: Morris
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Padula
authors.forename: Jason P
authors.initials: JP
authors.lastname: Mulvenna
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Rush
authors.forename: Jonathan
authors.initials: J
authors.lastname: Golledge
mesh_terms: D000818:Animals; D017544:Aortic Aneurysm, Abdominal / Q000235:genetics / Q000378:metabolism*; D053302:Apolipoprotein C-I / Q000235:genetics / Q000378:metabolism*; D001057:Apolipoproteins E / Q000235:genetics; D015415:Biomarkers / Q000378:metabolism; D002853:Chromatography, Liquid; D020869:Gene Expression Profiling; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D018613:Microscopy, Confocal; D053719:Tandem Mass Spectrometry
chemical_list: D053302:Apolipoprotein C-I; D001057:Apolipoproteins E; D015415:Biomarkers
keywords: Abdominal aortic aneurysm; Apolipoprotein C1; Biomarker; Mouse model