title: Enhanced energy conversion efficiency from high strength synthetic organic wastewater by sequential dark fermentative hydrogen production and algal lipid accumulation.
abstract: A two-stage process of sequential dark fermentative hydrogen production and microalgal cultivation was applied to enhance the energy conversion efficiency from high strength synthetic organic wastewater. Ethanol fermentation bacterium Ethanoligenens harbinense B49 was used as hydrogen producer, and the energy conversion efficiency and chemical oxygen demand (COD) removal efficiency reached 18.6% and 28.3% in dark fermentation. Acetate was the main soluble product in dark fermentative effluent, which was further utilized by microalga Scenedesmus sp. R-16. The final algal biomass concentration reached 1.98gL(-1), and the algal biomass was rich in lipid (40.9%) and low in protein (23.3%) and carbohydrate (11.9%). Compared with single dark fermentation stage, the energy conversion efficiency and COD removal efficiency of two-stage system remarkably increased 101% and 131%, respectively. This research provides a new approach for efficient energy production and wastewater treatment using a two-stage process combining dark fermentation and algal cultivation.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Hong-Yu
authors.initials: HY
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China. Electronic address: lbf@hit.edu.cn.
authors.forename: Bing-Feng
authors.initials: BF
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Fanying
authors.initials: F
authors.lastname: Kong
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Defeng
authors.initials: D
authors.lastname: Xing
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China. Electronic address: rnq@hit.edu.cn.
authors.forename: Nan-Qi
authors.initials: NQ
authors.lastname: Ren
mesh_terms: D000085:Acetates / Q000494:pharmacology; D001673:Biodegradation, Environmental / Q000187:drug effects; D057919:Biological Oxygen Demand Analysis; D001709:Biotechnology / Q000379:methods*; D003624:Darkness; D004736:Energy-Generating Resources; D000431:Ethanol / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D006859:Hydrogen / Q000378:metabolism*; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D009930:Organic Chemicals / Q000302:isolation & purification; D044444:Scenedesmus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D062065:Wastewater / Q000737:chemistry*
chemical_list: D000085:Acetates; D000431:Ethanol; D006859:Hydrogen; D008055:Lipids; D009930:Organic Chemicals; D062065:Wastewater
keywords: Algal cultivation; Biochemical composition; Hydrogen production; Lipid accumulation; Wastewater treatment