title: Global and quantitative profiling of polyadenylated RNAs using PAS-seq.
abstract: mRNA alternative polyadenylation (APA) has been increasingly recognized as a widespread and evolutionarily conserved mechanism for eukaryotic gene regulation. Here we describe a method called poly(A) site sequencing that can not only map RNA polyadenylation sites on a transcriptome level but also provide quantitative information on the relative abundance of polyadenylated RNAs. This method has been successfully used for both global APA analysis and digital gene expression profiling.
authors.affiliation: Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, Irvine, CA, USA.
authors.forename: Chengguo
authors.initials: C
authors.lastname: Yao
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Shi
mesh_terms: D059014:High-Throughput Nucleotide Sequencing; D026723:Polyadenylation / Q000502:physiology*; D012333:RNA, Messenger / Q000737:chemistry* / Q000235:genetics*
chemical_list: D012333:RNA, Messenger