title: Description of an advantageous optical label-free biosensing interferometric read-out method to measure biological species.
abstract: In this article we report a new, simple, and reliable optical read-out detection method able to assess Rotavirus present in human sera as well as in the viral pollution sources. It is based on the interference of two interferometers used as biophotonic transducers. The method significantly improves the optical label-free biosensing response measuring both, the concentration of the AgR and its corresponding size. Two different immunoassays were carried out: Bovine Serum Albumin (BSA), and the recognition by its antibody (anti-BSA); and Rotavirus (AgR) and the recognition by its antibody (anti-AgR). In the cases studied, and using as model interferometer a simple Fabry-Perot transducer, we demonstrate a biosensing enhancement of two orders of magnitude in the Limit of Detection (LoD). In fact, this read-out optical method may have significant implications to enhance other optical label-free photonic transducers reported in the scientific literature.
authors.affiliation: Micro-nano Photonics and Biophotonics Group, Centro Láser and Centro de Tecnología Biomédica, Universidad Politécnica de Madrid, Av. Ramiro de Maeztu 7, Madrid 28040, Spain. m.holgado@upm.es.
authors.forename: Miguel
authors.initials: M
authors.lastname: Holgado
authors.affiliation: Micro-nano Photonics and Biophotonics Group, Centro Láser and Centro de Tecnología Biomédica, Universidad Politécnica de Madrid, Av. Ramiro de Maeztu 7, Madrid 28040, Spain. fj.sanza@upm.es.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Sanza
authors.affiliation: Micro-nano Photonics and Biophotonics Group, Centro Láser and Centro de Tecnología Biomédica, Universidad Politécnica de Madrid, Av. Ramiro de Maeztu 7, Madrid 28040, Spain. alavin@etsii.upm.es.
authors.forename: Ana
authors.initials: A
authors.lastname: López
authors.affiliation: Micro-nano Photonics and Biophotonics Group, Centro Láser and Centro de Tecnología Biomédica, Universidad Politécnica de Madrid, Av. Ramiro de Maeztu 7, Madrid 28040, Spain. alavin@etsii.upm.es.
authors.forename: Alvaro
authors.initials: A
authors.lastname: Lavín
authors.affiliation: Micro-nano Photonics and Biophotonics Group, Centro Láser and Centro de Tecnología Biomédica, Universidad Politécnica de Madrid, Av. Ramiro de Maeztu 7, Madrid 28040, Spain. rafael.casquel@upm.es.
authors.forename: Rafael
authors.initials: R
authors.lastname: Casquel
authors.affiliation: Micro-nano Photonics and Biophotonics Group, Centro Láser and Centro de Tecnología Biomédica, Universidad Politécnica de Madrid, Av. Ramiro de Maeztu 7, Madrid 28040, Spain. mariafe.laguna@upm.es.
authors.forename: María F
authors.initials: MF
authors.lastname: Laguna
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D015374:Biosensing Techniques; D002417:Cattle; D006801:Humans; D007118:Immunoassay; D007368:Interferometry; D008027:Light; D017785:Photons; D012401:Rotavirus / Q000276:immunology / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000032:analysis* / Q000276:immunology; D059629:Signal-To-Noise Ratio
chemical_list: D000906:Antibodies; D012710:Serum Albumin, Bovine