title: Irisin: a potentially candidate marker for myocardial infarction.
abstract: Myocardial infarction (MI) causes energy depletion through imbalance between coronary blood supply and myocardial demand. Irisin produced by the heart reduces ATP production by increasing heat generation. Energy depletion affects irisin concentration in circulation and cardiac tissues, suggesting an association with MI. We examined: (1) irisin expression immunohistochemically in rat heart, skeletal muscle, kidney and liver in isoproterenol (ISO)-induced MI, and (2) serum irisin concentration by ELISA. Rats were randomly allocated into 6 groups (n=6), (i) control, (ii) ISO (1h), (iii) ISO (2h), (iv) ISO (4h), (v) ISO (6h), and (vi) ISO (24h), 200mg ISO in each case. Rats were decapitated and the blood and tissues collected for irisin analysis. Blood was centrifuged at 1792 g for 5 min. Tissues were washed with saline and fixed in 10% formalin for histology. Serum irisin levels gradually decreased from 1h to 24h in MI rats compared with controls, the minimum being at 2h, increasing again after 6h. Cardiac muscle cells, glomerular, peritubular renal cortical interstitial cells, hepatocytes and liver sinusoidal cells and perimysium, endomysium and nucleoi of skeletal muscle were irisin positive, but its synthesis decreased 1-4h after MI. At all time-points, irisin increased near myocardial connective tissue, with production in skeletal muscle, liver and kidney recovering after 6h, although slower than controls. Unique insight into the pathogenesis of MI is shown, and the gradually decrease of serum irisin might be a diagnostic marker for MI.
authors.affiliation: Firat University, School of Medicine, Department of Histology and Embryology, Elazig 23119, Turkey.
authors.forename: Tuncay
authors.initials: T
authors.lastname: Kuloglu
authors.affiliation: Department of Cardiovascular Surgery, Elazig Research and Education Hospital, Elazig 23100, Turkey; Firat University, School of Medicine, Department of Anatomy, Elazig 23119, Turkey.
authors.forename: Suna
authors.initials: S
authors.lastname: Aydin
authors.affiliation: Dicle University, School of Medicine, Department of Cardiovascular Surgery, Diyarbakir 21280, Turkey.
authors.forename: Mehmet Nesimi
authors.initials: MN
authors.lastname: Eren
authors.affiliation: Firat University, School of Medicine, Department of Medical Biochemistry (Firat Hormone Research Groups), Elazig 23119, Turkey.
authors.forename: Musa
authors.initials: M
authors.lastname: Yilmaz
authors.affiliation: Firat University, School of Medicine, Department of Medical Biochemistry (Firat Hormone Research Groups), Elazig 23119, Turkey; Erzincan University, School of Medicine, Department of Histology and Embryology, Erzincan 24030, Turkey.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Sahin
authors.affiliation: Firat University, School of Medicine, Department of Medical Biochemistry (Firat Hormone Research Groups), Elazig 23119, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Kalayci
authors.affiliation: Firat University, School of Medicine, Department of Histology and Embryology, Elazig 23119, Turkey.
authors.forename: Emine
authors.initials: E
authors.lastname: Sarman
authors.affiliation: Firat University, School of Medicine, Department of Histology and Embryology, Elazig 23119, Turkey.
authors.forename: Nalan
authors.initials: N
authors.lastname: Kaya
authors.affiliation: Firat University, School of Medicine, Department of Histology and Embryology, Elazig 23119, Turkey.
authors.forename: Osman Fatih
authors.initials: OF
authors.lastname: Yilmaz
authors.affiliation: Firat University, School of Medicine, Department of Histology and Embryology, Elazig 23119, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Turk
authors.affiliation: Ankara University, Faculty of Veterinary Medicine, Veterinary Medicine Student, Ankara 06110, Turkey.
authors.forename: Yalcin
authors.initials: Y
authors.lastname: Aydin
authors.affiliation: Firat University, Faculty of Veterinary Medicine, Department of Histology and Embryology, Elazig 23119, Turkey.
authors.forename: Mehmet Hanifi
authors.initials: MH
authors.lastname: Yalcin
authors.affiliation: Firat University, School of Medicine, Medical School Student, Elazig 23119, Turkey.
authors.forename: Nimet
authors.initials: N
authors.lastname: Uras
authors.affiliation: Firat University, School of Medicine, Department of Internal Medicine, Elazig 23119, Turkey.
authors.forename: Ali
authors.initials: A
authors.lastname: Gurel
authors.affiliation: Firat University, School of Medicine, Department of Medical Pharmacy, Elazig 23119, Turkey.
authors.forename: Selcuk
authors.initials: S
authors.lastname: Ilhan
authors.affiliation: Department of Emergency, Elazig education and Research Hospital, Elazig 23100, Turkey.
authors.forename: Evrim
authors.initials: E
authors.lastname: Gul
authors.affiliation: Firat University, School of Medicine, Department of Medical Biochemistry (Firat Hormone Research Groups), Elazig 23119, Turkey. Electronic address: saydin1@hotmail.com.
authors.forename: Suleyman
authors.initials: S
authors.lastname: Aydin
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D005353:Fibronectins / Q000097:blood*; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism; D009203:Myocardial Infarction / Q000097:blood*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D051381:Rats
chemical_list: D015415:Biomarkers; C583676:FNDC5 protein, rat; D005353:Fibronectins
keywords: Heart; Irisin; Isoproterenol; Kidneys; Myocardial infarction; Skeletal muscle