title: Electronic hybridization detection in microarray format and DNA genotyping.
abstract: We describe an approach to substituting a fluorescence microarray with a surface made of an arrangement of electrolyte-gated field effect transistors. This was achieved using a dedicated blocking of non-specific interactions and comparing threshold voltage shifts of transistors exhibiting probe molecules of different base sequence. We apply the approach to detection of the 35delG mutation, which is related to non-syndromic deafness and is one of the most frequent mutations in humans. The process involves barcode sequences that are generated by Tas-PCR, a newly developed replication reaction using polymerase blocking. The barcodes are recognized by hybridization to surface attached probes and are directly detected by the semiconductor device.
authors.affiliation: Laboratoire Nanobiophysique ESPCI ParisTech, CNRS UMR Gulliver 7083 10 rue Vauquelin, 75005 Paris, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Blin
authors.affiliation: Laboratoire Nanobiophysique ESPCI ParisTech, CNRS UMR Gulliver 7083 10 rue Vauquelin, 75005 Paris, France.
authors.forename: Ismaïl
authors.initials: I
authors.lastname: Cissé
authors.affiliation: Laboratoire Nanobiophysique ESPCI ParisTech, CNRS UMR Gulliver 7083 10 rue Vauquelin, 75005 Paris, France.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Bockelmann
mesh_terms: D001483:Base Sequence; D003217:Conductometry / Q000295:instrumentation*; D004247:DNA / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005838:Genotype; D017403:In Situ Hybridization / Q000295:instrumentation*; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D017422:Sequence Analysis, DNA / Q000295:instrumentation*; D014173:Transistors, Electronic
chemical_list: D004247:DNA