title: Amyloid peptides derived from CsgA and FapC modify the viscoelastic properties of biofilm model matrices.
abstract: The bacterial biofilm is a complex environment of cells, which secrete a matrix made of various components, mainly polysaccharides and proteins. An understanding of the precise role of these components in the stability and dynamics of biofilm architecture would be a great advantage for the improvement of anti-biofilm strategies. Here, artificial biofilm matrices made of polysaccharides and auto-assembled peptides were designed, and the influence of bacterial amyloid proteins on the mechanical properties of the biofilm matrix was studied. The model polysaccharides methylcellulose and alginate and peptides derived from the amyloid proteins curli and FapC found in biofilms of Enterobacteriaceae and Pseudomonas, respectively, were used. Rheological measurements showed that the amyloid peptides do not prevent the gelation of the polysaccharides but influence deformation of the matrices under shear stress and modify the gel elastic response. Hence the secretion of amyloids could be for the biofilm a way of adapting to environmental changes.
authors.affiliation: a Laboratoire ERRMECe-EA1391 , Institut des matériaux-FD4122, Université de Cergy-Pontoise , Cergy-Pontoise Cedex , France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Lembré
authors.forename: Patrick
authors.initials: P
authors.lastname: Di Martino
authors.forename: Charlotte
authors.initials: C
authors.lastname: Vendrely
mesh_terms: D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D052160:Benzothiazoles; D018441:Biofilms; D004548:Elasticity; D029968:Escherichia coli Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D017550:Spectroscopy, Fourier Transform Infrared; D013314:Stress, Mechanical; D013844:Thiazoles / Q000737:chemistry; D014783:Viscosity
chemical_list: D000682:Amyloid; D001426:Bacterial Proteins; D052160:Benzothiazoles; D029968:Escherichia coli Proteins; D013844:Thiazoles; C489688:csgA protein, E coli; C009462:thioflavin T