title: Synthesis of superparamagnetic iron oxide nanoparticles coated with a DDNP-carboxyl derivative for in vitro magnetic resonance imaging of Alzheimer's disease.
abstract: Superparamagnetic iron oxide nanoparticles (SPIONs) have been proposed for use in magnetic resonance imaging as versatile ultra-sensitive nanoprobes for Alzheimer's disease imaging. In this work, we synthetized an efficient contrast agent of Alzheimer's disease using 1,1-dicyano-2-[6-(dimethylamino)naphthalene-2-yl]propene (DDNP) carboxyl derivative to functionalize the surface of SPIONs. The DDNP-SPIONs are prepared by conjugating DDNP carboxyl derivative to oleic acid-treated SPIONs through ligand exchange. The structure, size distribution and magnetic property were identified by IR, TGA-DTA, XRD, TEM, Zetasizer Nano and VSM. TEM and Zetasizer Nano observations indicated that the DDNP-SPIONs are relatively mono-dispersed spherical distribution with an average size of 11.7nm. The DDNP-SPIONs were then further analyzed for their MRI relaxation properties using MR imaging and demonstrated high T2 relaxivity of 140.57s(-1)FemM(-1), and the vitro experiment that DDNP-SPIONs binding to β-Amyloid aggregates were then investigated by fluorophotometry, the results showed that the combination had induced the fluorescence enhancement of the DDNP-SPIONs and displayed tremendous promise for use as a contrast agent of Alzheimer's disease in MRI.
authors.affiliation: College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China.
authors.forename: Jingting
authors.initials: J
authors.lastname: Zhou
authors.affiliation: College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China. Electronic address: huanbaofa@cqu.edu.cn.
authors.forename: Huanbao
authors.initials: H
authors.lastname: Fa
authors.affiliation: College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yin
authors.affiliation: College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400037, China.
authors.forename: Changjun
authors.initials: C
authors.lastname: Hou
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400037, China.
authors.forename: Danqun
authors.initials: D
authors.lastname: Huo
authors.affiliation: Department of Radiology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China. Electronic address: hszhangd@163.com.
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Zhang
mesh_terms: D015081:2-Naphthylamine / Q000031:analogs & derivatives / Q000138:chemical synthesis; D000544:Alzheimer Disease / Q000000981:diagnostic imaging*; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000378:metabolism; D003287:Contrast Media / Q000138:chemical synthesis* / Q000737:chemistry; D003911:Dextrans / Q000737:chemistry*; D006801:Humans; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D011859:Radiography; D013499:Surface Properties
chemical_list: D016229:Amyloid beta-Peptides; D003287:Contrast Media; D003911:Dextrans; D058185:Magnetite Nanoparticles; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40); D015081:2-Naphthylamine; C065507:ferumoxides
keywords: Alzheimer's disease; DDNP carboxyl derivative; MRI; SPIONs; β-Amyloid