title: Loss of arbuscular mycorrhizal fungal diversity in trap cultures during long-term subculturing.
abstract: Long-term successional dynamics of an inoculum of arbuscular mycorrhizal fungi (AMF) associated with the maize rhizosphere (from traditionally managed agroecosystems in Los Tuxtlas, Veracruz, Mexico), was followed in Bracchiaria comata trap cultures for almost eight years. The results indicate that AMF diversity is lost following long-term subculturing of a single plant host species. Only the dominant species, Claroideoglomus etunicatum, persisted in pot cultures after 13 cycles. The absence of other morphotypes was demonstrated by an 18S rDNA survey, which confirmed that the sequences present solely belonged to C. etunicatum. Members of Diversisporales were the first to decrease in diversity, and the most persistent species belonged to Glomerales.
authors.affiliation: Laboratorio de organismos benéficos. Facultad de Ciencias Agrícolas. Universidad Veracruzana. Gonzalo Aguirre Beltrán S/N. Zona Universitaria. CP 91000. Xalapa, Veracruz, México.
authors.forename: Dora
authors.initials: D
authors.lastname: Trejo-Aguilar
authors.affiliation: Laboratorio de organismos benéficos. Facultad de Ciencias Agrícolas. Universidad Veracruzana. Gonzalo Aguirre Beltrán S/N. Zona Universitaria. CP 91000. Xalapa, Veracruz, México.
authors.forename: Liliana
authors.initials: L
authors.lastname: Lara-Capistrán
authors.affiliation: Instituto Politécnico Nacional (IPN). CIIDIR-Sinaloa. Departamento de Biotecnología Agrícola. Boulevard Juan de Dios Bátiz Paredes No. 250. CP 81101. AP280. Guasave, Sinaloa, México;
authors.forename: Ignacio E
authors.initials: IE
authors.lastname: Maldonado-Mendoza
authors.affiliation: Laboratorio de organismos benéficos. Facultad de Ciencias Agrícolas. Universidad Veracruzana. Gonzalo Aguirre Beltrán S/N. Zona Universitaria. CP 91000. Xalapa, Veracruz, México.
authors.forename: Ramón
authors.initials: R
authors.lastname: Zulueta-Rodríguez
authors.affiliation: Laboratorio de organismos benéficos. Facultad de Ciencias Agrícolas. Universidad Veracruzana. Gonzalo Aguirre Beltrán S/N. Zona Universitaria. CP 91000. Xalapa, Veracruz, México ; Red de Ecología Funcional. Instituto de Ecología A. C. Camino Antiguo a Coatepec No. 351, Congregación del Haya, Xalapa 91070 Veracruz, México.
authors.forename: Wendy
authors.initials: W
authors.lastname: Sangabriel-Conde
authors.affiliation: Instituto Politécnico Nacional (IPN). CIIDIR-Sinaloa. Departamento de Biotecnología Agrícola. Boulevard Juan de Dios Bátiz Paredes No. 250. CP 81101. AP280. Guasave, Sinaloa, México;
authors.forename: María Elena
authors.initials: ME
authors.lastname: Mancera-López
authors.affiliation: Red de Ecología Funcional. Instituto de Ecología A. C. Camino Antiguo a Coatepec No. 351, Congregación del Haya, Xalapa 91070 Veracruz, México.
authors.forename: Simoneta
authors.initials: S
authors.lastname: Negrete-Yankelevich
authors.affiliation: Red de Ecología Funcional. Instituto de Ecología A. C. Camino Antiguo a Coatepec No. 351, Congregación del Haya, Xalapa 91070 Veracruz, México.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Barois
keywords: AM fungi; Diversisporales; Glomerales; preservation; rhizosphere