title: Embedding fluorescent mesoporous silica nanoparticles into biocompatible nanogels for tumor cell imaging and thermo/pH-sensitive in vitro drug release.
abstract: Thermo/pH-sensitive/fluorescent/biocompatible nanospheres consisting of quantum dots-embedded mesoporous silica nanoparticles (Q-MS) as a core and poly(N-isopropylacrylamide (NIPAM))-graft-chitosan (CS) nanogels as a shell (PNIPAM-g-CS) were prepared via temperature-regulated one-pot copolymerization of NIPAM monomer and CS in the presence of Q-MS. The prepared nanospheres exhibited remarkable fluorescence/thermo/pH-sensitivity. HepG2 cells treated with nanospheres displayed bright fluorescence imaging. Loading efficiency and capacity of Doxorubicin (Dox) into nanospheres were regularly increased with the increment of Dox concentration. At a high temperature and a low pH, cumulative in vitro release of Dox from Dox-loaded nanospheres was much great and fast. Released Dox still retained high anticancer activity, and blank nanosphere carriers produced neglectful toxicity to HepG2 cells. The multifunctional nanospheres could be further developed toward temperature/pH-regulated drug carriers for in vivo tumor therapy with a rapid drug release and fluorescence imaging in targeted tissues.
authors.affiliation: Institute of Materia Medica, Shandong Academy of Medical Sciences, Jinan 250062, PR China; Department of Chemistry, School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Rijun
authors.initials: R
authors.lastname: Gui
authors.affiliation: Institute of Materia Medica, Shandong Academy of Medical Sciences, Jinan 250062, PR China.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Materia Medica, Shandong Academy of Medical Sciences, Jinan 250062, PR China. Electronic address: sunjiesams@163.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D005453:Fluorescence; D056945:Hep G2 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D000080385:Nanogels; D053758:Nanoparticles / Q000737:chemistry; D009369:Neoplasms / Q000175:diagnosis* / Q000188:drug therapy*; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D016062:Porosity; D045663:Quantum Dots; D012822:Silicon Dioxide / Q000737:chemistry; D013329:Structure-Activity Relationship; D013499:Surface Properties; D013696:Temperature
chemical_list: D000970:Antineoplastic Agents; D001672:Biocompatible Materials; D000080385:Nanogels; C413692:polyethylene glycol polyethyleneimine nanogel; D011092:Polyethylene Glycols; D012822:Silicon Dioxide; D004317:Doxorubicin; D011094:Polyethyleneimine
keywords: Cell imaging; Chitosan; Drug release; N-Isopropylacrylamide; Nanospheres