title: Freeze-drying of ovalbumin loaded mesoporous silica nanoparticle vaccine formulation increases antigen stability under ambient conditions.
abstract: Amino functionalised mesoporous silica nanoparticles (AM-41) have been identified as a promising vaccine delivery material. The capacity of AM-41 to stabilise vaccine components at ambient temperature (23-27°C) was determined by adsorbing the model antigen ovalbumin (OVA) to AM-41 particles (OVA-41). The OVA-41 was successfully freeze-dried using the excipients 5% trehalose and 1% PEG8000. The immunological activity of OVA and the nanoparticle structure were maintained following two months storage at ambient temperature. The results of immunisation studies in mice with reconstituted OVA-41 demonstrated the induction of humoral and cell-meditated immune responses. The capacity of AM-41 particles to facilitate ambient storage of vaccine components without the loss of immunological potency will underpin the further development of this promising vaccine delivery platform.
authors.affiliation: Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Queensland, Australia. Electronic address: k.mody@uq.edu.au.
authors.forename: Karishma T
authors.initials: KT
authors.lastname: Mody
authors.affiliation: Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Queensland, Australia. Electronic address: d.mahony1@uq.edu.au.
authors.forename: Donna
authors.initials: D
authors.lastname: Mahony
authors.affiliation: Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Queensland, Australia. Electronic address: a.cavallaro@uq.edu.au.
authors.forename: Antonino S
authors.initials: AS
authors.lastname: Cavallaro
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Queensland, Australia. Electronic address: s4075611@student.uq.edu.au.
authors.forename: Frances
authors.initials: F
authors.lastname: Stahr
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Queensland, Australia. Electronic address: s.qiao@adelaide.edu.au.
authors.forename: Shi Zhang
authors.initials: SZ
authors.lastname: Qiao
authors.affiliation: Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Queensland, Australia. Electronic address: t.mahony@uq.edu.au.
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Mahony
authors.affiliation: Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Queensland, Australia. Electronic address: n.mitter@uq.edu.au.
authors.forename: Neena
authors.initials: N
authors.lastname: Mitter
mesh_terms: D000818:Animals; D000941:Antigens / Q000008:administration & dosage / Q000737:chemistry* / Q000276:immunology; D002417:Cattle; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D004337:Drug Carriers; D004355:Drug Stability; D004356:Drug Storage; D005079:Excipients / Q000737:chemistry; D005612:Freeze Drying; D007111:Immunity, Cellular / Q000187:drug effects; D056724:Immunity, Humoral / Q000187:drug effects; D007114:Immunization; D007279:Injections, Subcutaneous; D008810:Mice, Inbred C57BL; D053758:Nanoparticles; D036103:Nanotechnology; D010047:Ovalbumin / Q000008:administration & dosage / Q000737:chemistry* / Q000276:immunology; D011092:Polyethylene Glycols / Q000737:chemistry; D016062:Porosity; D055550:Protein Stability; D012822:Silicon Dioxide / Q000008:administration & dosage / Q000737:chemistry* / Q000633:toxicity; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D014199:Trehalose / Q000737:chemistry; D014612:Vaccines / Q000008:administration & dosage / Q000737:chemistry* / Q000276:immunology
chemical_list: D000941:Antigens; D004337:Drug Carriers; D005079:Excipients; D014612:Vaccines; D011092:Polyethylene Glycols; D012822:Silicon Dioxide; D010047:Ovalbumin; D014199:Trehalose
keywords: Excipients; Freeze-drying; Mesoporous silica nanoparticles; Protein; Stability; Vaccine delivery