title: The expression of aquaporins 1 and 5 in rat lung after thoracic irradiation.
abstract: Radiation-induced lung toxicity (RILT), leading to radiation pneumonia or fibrosis, is a primary problem of radiation therapy. The pathogenesis of RILT remains unclear. In this study, we used a rat model of RILT to examine the expression of aquaporins (AQPs) after radiation injury. Sprague Dawley rats were given a single dose of 17 Gy (dose rate of 3.0 Gy/min) of X-irradiation to the thorax. Rats that survived acute pneumonitis (at 1-4 weeks) were evaluated weekly for the expression of AQP1 and AQP5 in the lung by immunohistochemical and reverse transcription polymerase chain reaction (RT-PCR) analyses. Immunohistochemical analysis showed that AQP1 protein was expressed in the capillary endothelium, and its level was significantly decreased after irradiation. AQP5 protein was expressed in the alveolar epithelium, and its level was increased between Days 7 and 14 after irradiation but decreased at Day 28, compared with the sham group. The RT-PCR results were consistent with the immunohistochemical analysis results. In summary, this study provides the first report of AQP1 and AQP5 expression in a model of radiation-induced pulmonary inflammation and edema. Decreased levels of AQP1 and AQP5 after irradiation suggest that these proteins play a role in the pathogenesis of RILT.
authors.affiliation: Department of Radiation Oncology, The Fourth Affiliated Hospital of China Medical University, No. 4 Chongshan Road, Huanggu District, Shenyang 110032, China.
authors.forename: Cheng-Ying
authors.initials: CY
authors.lastname: Sun
authors.affiliation: Department of Radiation Oncology, The Fourth Affiliated Hospital of China Medical University, No. 4 Chongshan Road, Huanggu District, Shenyang 110032, China zyx_yd@163.com 2448356540@qq.com.
authors.forename: Yu-Xia
authors.initials: YX
authors.lastname: Zhao
authors.affiliation: Department of Radiation Oncology, The Fourth Affiliated Hospital of China Medical University, No. 4 Chongshan Road, Huanggu District, Shenyang 110032, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhong
authors.affiliation: Department of Radiation Oncology, The Fourth Affiliated Hospital of China Medical University, No. 4 Chongshan Road, Huanggu District, Shenyang 110032, China.
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Liu
authors.affiliation: Department of Radiation Oncology, The Fourth Affiliated Hospital of China Medical University, No. 4 Chongshan Road, Huanggu District, Shenyang 110032, China.
authors.forename: Yan-Zhi
authors.initials: YZ
authors.lastname: Chen
authors.affiliation: Department of Radiation Oncology, The Fourth Affiliated Hospital of China Medical University, No. 4 Chongshan Road, Huanggu District, Shenyang 110032, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Qin
authors.affiliation: Department of Radiation Oncology, The First Affiliated Hospital of China Medical University, No. 155 Nanjing Street, Heping District, Shenyang 110001, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Bai
authors.affiliation: Department of Radiation Oncology, The Fourth Affiliated Hospital of China Medical University, No. 4 Chongshan Road, Huanggu District, Shenyang 110032, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
mesh_terms: D000818:Animals; D051398:Aquaporin 1 / Q000235:genetics / Q000378:metabolism*; D051402:Aquaporin 5 / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression / Q000528:radiation effects; D007150:Immunohistochemistry; D008168:Lung / Q000378:metabolism* / Q000473:pathology / Q000528:radiation effects*; D055370:Lung Injury / Q000209:etiology / Q000235:genetics / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011833:Radiation Injuries, Experimental / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C496022:Aqp1 protein, rat; C496040:Aqp5 protein, rat; D051402:Aquaporin 5; D012333:RNA, Messenger; D051398:Aquaporin 1
keywords: AQP1; AQP5; radiation-induced lung toxicity