title: The effect of catalase on migration and invasion of lung cancer cells by regulating the activities of cathepsin S, L, and K.
abstract: Abundant clinical evidences indicate that up-regulation of several cathepsins in many human cancers is correlated with malignant progression and poor patient prognosis. In addition, a decrease in catalase activity or accumulation of hydrogen peroxide correlates with cancer metastasis. Recent studies indicate that cathepsin activation and expression can be modulated via H2O2 treatment. However, the actual relationship between catalase and cathepsins is not yet fully understood. In the present study, we found that catalase expression (or activity) was higher, while intracellular and extracellular Cat S, Cat L, and Cat K activities were lower in the non-invasive CL1-0 cells compared to the highly invasive CL1-5 cells. After CL1-0 cells were transfected with catalase-shRNA, the corresponding ROS (H2O2) level and Cat S, Cat L, or Cat K expression (or activity) was up-regulated, accompanied by an increase in cell migration and invasion. On the other hand, ROS (H2O2) level, cathepsin S, L, and K activities, cell migration and invasion were decreased in catalase-overexpressed CL1-5 cells. It is suggested that catalase may regulate cathepsin activity by controlling the production of ROS (H2O2), leading to variation in migration and invasion ability of lung cancer cells.
authors.affiliation: Institute of Biotechnology and Department of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan.
authors.forename: Ju-Ying
authors.initials: JY
authors.lastname: Tsai
authors.affiliation: Department of Bioscience Technology, Chang Jung Christian University, Tainan 71101, Taiwan.
authors.forename: Mon-Juan
authors.initials: MJ
authors.lastname: Lee
authors.affiliation: Institute of Molecular and Cellular Biology & Department of Medical Science, National Tsing Hua University, 101, Section 2, Kuang Fu Road, Hsinchu 30013, Taiwan. Electronic address: lscmdt@life.nthu.edu.tw.
authors.forename: Margaret
authors.initials: M
authors.lastname: Dah-Tsyr Chang
authors.affiliation: Institute of Biotechnology and Department of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan. Electronic address: hmhuang@life.nthu.edu.tw.
authors.forename: Haimei
authors.initials: H
authors.lastname: Huang
mesh_terms: D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D056657:Cathepsin K / Q000235:genetics / Q000378:metabolism*; D056668:Cathepsin L / Q000235:genetics / Q000378:metabolism*; D002403:Cathepsins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D008175:Lung Neoplasms / Q000201:enzymology* / Q000473:pathology*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D034622:RNA Interference; D034741:RNA, Small Interfering; D017382:Reactive Oxygen Species / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D034741:RNA, Small Interfering; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D002374:Catalase; D002403:Cathepsins; D056668:Cathepsin L; C028217:cathepsin S; D056657:Cathepsin K
keywords: Catalase; Cysteine cathepsin; H(2)O(2); Invasion; Lung adenocarcinoma; Migration