title: Methylene blue based device for pathogen reduction in human plasma.
abstract: BACKGROUND
  Despite improvement in safety of plasma transfusion some virus transmission still remains a problem. So as World Health Organization (WHO) recommends, many countries developed Pathogen Reduction Technologies (PRT) to inactivate pathogens, in plasma components. The Methylene Blue (MB) based methods is one of the most universal one. The purpose of this research was, produce a device that can inactivate viruses in MB environment.
  
  
  METHODS
  In this interventional study, each Plasma Sample was illuminated by 70Pieces (PCs) of 1 w red Light Emitting Diodes (LEDs) from one side. These LEDs emit light at central wavelength of 627 nm with 20 nm Full Width at Half Maximum (FWHM). Two model viruses Herpes Simplex Virus (HSV) and Vesicular Stomatitis Virus (VSV) were used and Tissue Culture 50% Infection Dose (TCID50) was used to calculate virus Log reduction. Two concentration of MB and 5 different illumination times were used.
  
  
  RESULTS
  In 10 µm concentration of MB, HSV had 6.00±0.2 maximum log reduction that obtain after 60 minutes illumination and VSV had 5.50± 0.3 maximum log reduction after 75 minutes illumination. In 1 µM concentration of MB, HSV had 5.20±0.3 maximum log reduction that obtain after 60 minutes illumination and VSV had 4.90± 0.2 maximum log reduction after 75 minutes illumination.
  
  
  CONCLUSIONS
  Results of virus inactivation in this method were similar to other methods (P-value<0.05 in comparison with Spring method, and P-value>0.05 in comparison with Theraflex), and it showed this device could inactivate viruses according to WHO recommendation.
authors.affiliation: Virology Laboratory, Research Centre of Iranian Blood Transfusion Organization (IBTO), Tehran, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Elikaei
authors.affiliation: Department of Microbiology, Faculty of Biological Science Shahid Beheshti University, GC, Tehran, Iran.
authors.forename: S M
authors.initials: SM
authors.lastname: Hosseini
authors.affiliation: Virology Laboratory, Research Centre of Iranian Blood Transfusion Organization (IBTO), Tehran, Iran.
authors.forename: Z
authors.initials: Z
authors.lastname: Sharifi
authors.affiliation: Laser and Plasma Research Institute, Shahid Beheshti University, GC, Tehran, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Latifi
authors.affiliation: Laser and Plasma Research Institute, Shahid Beheshti University, GC, Tehran, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Nikbakht
authors.affiliation: Department of Microbiology, Faculty of Biological Science Shahid Beheshti University, GC, Tehran, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Mirshafiee
authors.affiliation: Laser and Plasma Research Institute, Shahid Beheshti University, GC, Tehran, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Asadollahi
keywords: Blood-borne Pathogens; Methylene Blue; Plasma