title: Improved lanthipeptide detection and prediction for antiSMASH.
abstract: Lanthipeptides are a class of ribosomally synthesised and post-translationally modified peptide (RiPP) natural products from the bacterial secondary metabolism. Their name is derived from the characteristic lanthionine or methyl-lanthionine residues contained in the processed peptide. Lanthipeptides that possess an antibacterial activity are called lantibiotics. Whereas multiple tools exist to identify lanthipeptide gene clusters from genomic data, no programs are available to predict the post-translational modifications of lanthipeptides, such as the proteolytic cleavage of the leader peptide part or tailoring modifications based on the analysis of the gene cluster sequence. antiSMASH is a software pipeline for the identification of secondary metabolite biosynthetic clusters from genomic input and the prediction of products produced by the identified clusters. Here we present a novel antiSMASH module using a rule-based approach to combine signature motifs for biosynthetic enzymes and lanthipeptide-specific cleavage site motifs to identify lanthipeptide clusters in genomic data, assign the specific lanthipeptide class, predict prepeptide cleavage, tailoring reactions, and the processed molecular weight of the mature peptide products.
authors.affiliation: Microbiology/Biotechnology, Interfaculty Institute of Microbiology and Infection Medicine, Eberhard-Karls-University of Tübingen, Tübingen, Germany ; German Centre for Infection Research, Tübingen, Germany.
authors.forename: Kai
authors.initials: K
authors.lastname: Blin
authors.affiliation: Microbiology/Biotechnology, Interfaculty Institute of Microbiology and Infection Medicine, Eberhard-Karls-University of Tübingen, Tübingen, Germany.
authors.forename: Daniyal
authors.initials: D
authors.lastname: Kazempour
authors.affiliation: Microbiology/Biotechnology, Interfaculty Institute of Microbiology and Infection Medicine, Eberhard-Karls-University of Tübingen, Tübingen, Germany ; German Centre for Infection Research, Tübingen, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Wohlleben
authors.affiliation: Microbiology/Biotechnology, Interfaculty Institute of Microbiology and Infection Medicine, Eberhard-Karls-University of Tübingen, Tübingen, Germany ; German Centre for Infection Research, Tübingen, Germany.
authors.forename: Tilmann
authors.initials: T
authors.lastname: Weber
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000032:analysis*; D001430:Bacteriocins / Q000032:analysis* / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D010446:Peptide Fragments / Q000032:analysis*; D011499:Protein Processing, Post-Translational; D012984:Software
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D010446:Peptide Fragments