title: Biosurfactant stabilized anticancer biomolecule-loaded poly (D,L-lactide) nanoparticles.
abstract: PLA nanoparticles (NPs) were prepared via green route using turmeric (Curcuma longa) extract (TE) as biostabiliser/biosurfactant. Of the 29 formulations, two formulations of TE synthesized PLA NPs were evaluated for encapsulation and controlled release of well known antioxidant and less bioavailable molecules curcumin and quercetin. Size of curcumin loaded PLA NPs synthesized using 0.8 mg/ml PLA (C-En-D) and 0.1 mg/ml PLA (C-En-P) were 203±77 and 110±44 nm, respectively. However, quercetin loaded PLA NPs synthesized at 0.8 mg/ml (Q-En-D) and 0.1mg/ml (Q-En-P) PLA concentrations were 170±95 and 102±31 nm, respectively. The encapsulation efficiency of curcumin loaded PLA (C-En-D and C-En-P) NPs as well as quercetin loaded PLA (Q-En-D and Q-En-P) NPs was found ∼95%. In vitro release study of C-En-D, C-En-P, Q-En-D and Q-En-P NPs showed initial burst release followed by slow and sustained release. C-En-D NPs and Q-En-D NPs showing maximum in vitro release (∼100%) were evaluated for cytotoxicity. Blank PLA NPs, C-En-D and Q-En-D NPs were found to be safe against normal human leukocytes up to 2 mg/ml dose. Both C-En-D and Q-En-D NPs showed anticancer activity against A549 cell line. But Q-En-D NPs showed better anticancer activity than C-En-D NPS on A549 cells. While blank PLA NPs did not possess anticancer activity. TE extract stabilized PLA NPs were non-toxic, biocompatible and safe to normal human leukocytes. Such technology will be better, effective and safer in use for anticancer as well as other biological application.
authors.affiliation: Biotechnology Division, CSIR, Institute of Himalayan Bioresource Technology, Council of Scientific and Industrial Research, Palampur 176061, HP, India.
authors.forename: Vineet
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Biotechnology Division, CSIR, Institute of Himalayan Bioresource Technology, Council of Scientific and Industrial Research, Palampur 176061, HP, India.
authors.forename: Avnesh
authors.initials: A
authors.lastname: Kumari
authors.affiliation: Biotechnology Division, CSIR, Institute of Himalayan Bioresource Technology, Council of Scientific and Industrial Research, Palampur 176061, HP, India.
authors.forename: Dharmesh
authors.initials: D
authors.lastname: Kumar
authors.affiliation: Biotechnology Division, CSIR, Institute of Himalayan Bioresource Technology, Council of Scientific and Industrial Research, Palampur 176061, HP, India. Electronic address: sudeshkumar@ihbt.res.in.
authors.forename: Sudesh Kumar
authors.initials: SK
authors.lastname: Yadav
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D003474:Curcumin / Q000494:pharmacology*; D006801:Humans; D008027:Light; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010936:Plant Extracts / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D011794:Quercetin / Q000494:pharmacology*; D012542:Scattering, Radiation; D013056:Spectrophotometry, Ultraviolet; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts; D011091:Polyesters; D013501:Surface-Active Agents; C033616:poly(lactide); D011794:Quercetin; D003474:Curcumin
keywords: Anticancer; Controlled release; Curcumin; Green synthesis; Poly (d,l-lactide) (PLA); Quercetin