title: Human enteroids: preclinical models of non-inflammatory diarrhea.
abstract: Researchers need an available and easy-to-use model of the human intestine to better understand human intestinal physiology and pathophysiology of diseases, and to offer an enhanced platform for developing drug therapy. Our work employs human enteroids derived from each of the major intestinal sections to advance understanding of several diarrheal diseases, including those caused by cholera, rotavirus and enterohemorrhagic Escherichia coli. An enteroid bank is being established to facilitate comparison of segmental, developmental, and regulatory differences in transport proteins that can influence therapy efficacy. Basic characterization of major ion transport protein expression, localization and function in the human enteroid model sets the stage to study the effects of enteric infection at the transport level, as well as to monitor potential responses to pharmacological intervention.
authors.forename: Olga
authors.initials: O
authors.lastname: Kovbasnjuk
authors.forename: Nicholas C
authors.initials: NC
authors.lastname: Zachos
authors.forename: Julie
authors.initials: J
authors.lastname: In
authors.forename: Jennifer
authors.initials: J
authors.lastname: Foulke-Abel
authors.forename: Khalil
authors.initials: K
authors.lastname: Ettayebi
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Hyser
authors.forename: James R
authors.initials: JR
authors.lastname: Broughman
authors.forename: Xi-Lei
authors.initials: XL
authors.lastname: Zeng
authors.forename: Sabine
authors.initials: S
authors.lastname: Middendorp
authors.forename: Hugo R
authors.initials: HR
authors.lastname: de Jonge
authors.forename: Mary K
authors.initials: MK
authors.lastname: Estes
authors.forename: Mark
authors.initials: M
authors.lastname: Donowitz
mesh_terms: D003967:Diarrhea / Q000473:pathology; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology* / Q000821:virology; D017136:Ion Transport; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D008954:Models, Biological; D012401:Rotavirus / Q000254:growth & development / Q000502:physiology; D012964:Sodium / Q000378:metabolism
chemical_list: D012964:Sodium