title: Cysteine proteinase-1 and cut protein isoform control dendritic innervation of two distinct sensory fields by a single neuron.
abstract: Dendrites often exhibit structural changes in response to local inputs. Although mechanisms that pattern and maintain dendritic arbors are becoming clearer, processes regulating regrowth, during context-dependent plasticity or after injury, remain poorly understood. We found that a class of Drosophila sensory neurons, through complete pruning and regeneration, can elaborate two distinct dendritic trees, innervating independent sensory fields. An expression screen identified Cysteine proteinase-1 (Cp1) as a critical regulator of this process. Unlike known ecdysone effectors, Cp1-mutant ddaC neurons pruned larval dendrites normally but failed to regrow adult dendrites. Cp1 expression was upregulated/concentrated in the nucleus during metamorphosis, controlling production of a truncated Cut homeodomain transcription factor. This truncated Cut, but not the full-length protein, allowed Cp1-mutant ddaC neurons to regenerate higher-order adult dendrites. These results identify a molecular pathway needed for dendrite regrowth after pruning, which allows the same neuron to innervate distinct sensory fields.
authors.affiliation: Department of Cell Biology, Duke University School of Medicine, Durham, NC 27710, USA.|Medical Scientist Training Program, Duke University School of Medicine, Durham, NC 27710, USA.
authors.forename: Gray R
authors.initials: GR
authors.lastname: Lyons
authors.affiliation: Department of Cell Biology, Duke University School of Medicine, Durham, NC 27710, USA.
authors.forename: Ryan O
authors.initials: RO
authors.lastname: Andersen
authors.affiliation: Department of Cell Biology, Duke University School of Medicine, Durham, NC 27710, USA.
authors.forename: Khadar
authors.initials: K
authors.lastname: Abdi
authors.affiliation: Department of Cell Biology, Duke University School of Medicine, Durham, NC 27710, USA.
authors.forename: Won-Seok
authors.initials: WS
authors.lastname: Song
authors.affiliation: Department of Cell Biology, Duke University School of Medicine, Durham, NC 27710, USA.|Brumley Neonatal-Perinatal Research Institute, Department of Pediatrics, Duke University School of Medicine, Durham, NC 27710, USA.|Department of Neurobiology, Duke University School of Medicine, Durham, NC 27710, USA.|Preston Robert Tisch Brain Tumor Center, Duke University School of Medicine, Durham, NC 27710, USA.|Duke Institute for Brain Sciences, Duke University School of Medicine, Durham, NC 27710, USA.
authors.forename: Chay T
authors.initials: CT
authors.lastname: Kuo
mesh_terms: D000818:Animals; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D003712:Dendrites / Q000201:enzymology*; D004330:Drosophila; D029721:Drosophila Proteins / Q000378:metabolism*; D020033:Protein Isoforms; D011984:Sensory Receptor Cells / Q000201:enzymology*
chemical_list: D029721:Drosophila Proteins; D020033:Protein Isoforms; D003546:Cysteine Endopeptidases; C106944:Cysteine proteinase-1, Drosophila