title: Salvage or recovery of failed targets by in situ proteolysis.
abstract: In situ proteolysis is the method of proactively adding tiny amounts of nonspecific proteases to aid in the crystallization of proteins and protein macromolecular complexes. The simplicity of the procedure and high recovery rate make it a method of first choice for recalcitrant targets. An improved and updated in situ proteolysis protocol used in high-throughput structural biology platforms is described.
authors.affiliation: Structural Genomics Consortium, University of Toronto, 101 College Street, MaRS Centre, South Tower, Suite 700, Toronto, ON, Canada, M5G 1L7, yufeng.tong@utoronto.ca.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Tong
authors.forename: Aiping
authors.initials: A
authors.lastname: Dong
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Xu
authors.forename: Amy
authors.initials: A
authors.lastname: Wernimont
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D057166:High-Throughput Screening Assays; D008967:Molecular Biology / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D059748:Proteolysis
chemical_list: D011506:Proteins