title: A staining method for assessing the viability of Esteya vermicola conidia.
abstract: The viability of conidia of Esteya vermicola, a potentially important biocontrol agent against the pinewood nematode Bursaphelenchus xylophilus, is usually determined by cultivation for 18-48 h in culture medium. As an alternative to this labor-intensive method, we have developed a rapid, simple, and low-cost staining method for assessing E vermicola conidia survival rates. A mixture of neutral red and methylene blue was found to be the most optimal among several stains that also included safranin O and Janus green B. This mixture stained nonviable conidia blue, in contrast to viable conidia, which were stained red in the cytoplasm and blue in the cell wall. This method may be particularly useful for traditional research laboratories, as it provides rapid results using common, relatively inexpensive laboratory equipment.
authors.affiliation: Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University, Daejeon, 305-764, South Korea, fighting_bobo@hotmail.com.
authors.forename: Yunbo
authors.initials: Y
authors.lastname: Wang
authors.forename: NguyenTrong
authors.initials: N
authors.lastname: Thang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
authors.forename: Yongan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jingjie
authors.initials: J
authors.lastname: Li
authors.forename: Jianjie
authors.initials: J
authors.lastname: Xue
authors.forename: Lijuan
authors.initials: L
authors.lastname: Gu
authors.forename: VuThuy
authors.initials: V
authors.lastname: Hong
authors.forename: Lee
authors.initials: L
authors.lastname: Mira
authors.forename: Changkeun
authors.initials: C
authors.lastname: Sung
mesh_terms: D000818:Animals; D001391:Azo Compounds; D061046:Biological Control Agents; D004396:Coloring Agents; D055155:Ophiostomatales / Q000502:physiology*; D010619:Phenazines; D028223:Pinus / Q000469:parasitology*; D010935:Plant Diseases / Q000517:prevention & control*; D013172:Spores, Fungal / Q000502:physiology*; D013194:Staining and Labeling; D017187:Tylenchida / Q000382:microbiology*
chemical_list: D001391:Azo Compounds; D061046:Biological Control Agents; D004396:Coloring Agents; D010619:Phenazines; C005086:Janus Green B; C009195:safranine T