title: [Separation of amino acid enantiomers by high performance liquid chromatography].
abstract: Enantiomeric forms of the target analytes can be separated using chiral stationary phases based on cyclodextrins, macrocyclic polyethers and antibiotics, and derivatized polysaccharides and cyclofructans. The polar-organic, reverse-phase and normal-phase separation system of the mobile phase can be used for chromatographic separation of racemic mixtures of amino acids, depending on the type of the chiral stationary phase. In addition to the direct method of separation, the possibility of derivatization with a chiral derivatization agent is used. The conventional detection techniques, circular dichroism and laser polarimetry detection can be used to detect amino acids after HPLC separation.
authors.forename: Jakub
authors.initials: J
authors.lastname: Moravčík
authors.forename: Katarína
authors.initials: K
authors.lastname: Hroboňová
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003505:Cyclodextrins / Q000737:chemistry; D007202:Indicators and Reagents / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids; D000900:Anti-Bacterial Agents; D003505:Cyclodextrins; D007202:Indicators and Reagents; D011134:Polysaccharides