title: New karyotypes of Atlantic tree rats, genus Phyllomys (Rodentia: Echimyidae).
abstract: Phyllomys (Echimyidae, Rodentia) is a genus of Neotropical rodents with available cytogenetic data restricted to six out of 13 species, mainly based on simple staining methods, without detailed analyses. In this work, we present new karyotypes for Phyllomys lamarum (diploid number 2n = 56, fundamental number or number of autosomal arms FN = 102) and Phyllomys sp. (2n = 74, FN = 140) from the state of Minas Gerais, southeastern Brazil. We provide the first GTG- and CBG-banding patterns, silver-staining of the nucleolar organizer regions (Ag-NORs), and fluorescence in situ hybridization (FISH) with telomeric and 45S rDNA probes of Phyllomys. In addition to examining their chromosomes and phenotypic characters, we sequenced mitochondrial DNA from the specimens analyzed to confirm their taxonomic identification. The comparison of the distinctive chromosome complements of our specimens with those of other species of Phyllomys already published allowed us to conclude that chromosome data may be very useful for the taxonomy of the genus, as no two species analyzed presented the same diploid and fundamental numbers (2n and FN).
authors.affiliation: a Universidade Federal de Minas Gerais, Laboratório de Citogenômica Evolutiva, Departamento de Biologia Geral, Instituto de Ciências Biológicas, Avenida Presidente Antônio Carlos, 6627 - Pampulha, 31270-901. Belo Horizonte, Brazil.
authors.forename: Naiara Pereira
authors.initials: NP
authors.lastname: Araújo
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: Loss
authors.forename: Dirceu A
authors.initials: DA
authors.lastname: Cordeiro-Junior
authors.forename: Kátia Regina
authors.initials: KR
authors.lastname: da Silva
authors.forename: Yuri L R
authors.initials: YL
authors.lastname: Leite
authors.forename: Marta
authors.initials: M
authors.lastname: Svartman
mesh_terms: D000818:Animals; D001938:Brazil; D002871:Chromosome Banding; D033481:Chromosomes, Mammalian / Q000235:genetics; D020732:Cytogenetic Analysis / Q000379:methods*; D004272:DNA, Mitochondrial / Q000235:genetics; D019143:Evolution, Molecular; D005260:Female; D017404:In Situ Hybridization, Fluorescence; D059785:Karyotype; D008297:Male; D010802:Phylogeny; D051381:Rats; D012377:Rodentia / Q000145:classification* / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial