title: Biodegradation of neonicotinoid insecticide, imidacloprid by restriction enzyme mediated integration (REMI) generated Trichoderma mutants.
abstract: REMI (restriction enzyme-mediated integration) technique was employed to construct Trichoderma atroviride strain T23 mutants with degrading capability of neonicotinoid insecticide, imidacloprid. The plasmid pBluescript II KS-hph used for integration in REMI mutants was confirmed by PCR and Southern hybridization. Among 153 transformants, 57% of them have showed higher neonicotinoid insecticide, imidacloprid, degradation ability than the wild strain T23 (p<0.01). More specifically, seven single-copied T. atroviride T23 transformants have confirmed a 30% higher degradation rate than their parent isolate. Among all transformed mutants, a 95% imidacloprid degradation rate was identified as the highest. This study, thus, provided an effective approach for improving neonicotinoid insecticide-degrading capability using REMI transformed T. atroviride mutants.
authors.affiliation: Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, PR China. Electronic address: hxf791230@163.com.
authors.forename: Xiangfeng
authors.initials: X
authors.lastname: He
authors.affiliation: Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, PR China; Sekota Dryland Agricultural Research Center, Institute of Amhara Agricultural Research, Amhara Region, Sekota 62, Ethiopia. Electronic address: ajenberie@gmail.com.
authors.forename: Abebe Jenberie
authors.initials: AJ
authors.lastname: Wubie
authors.affiliation: Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, PR China. Electronic address: dqyun1@126.com.
authors.forename: Qingyun
authors.initials: Q
authors.lastname: Diao
authors.affiliation: Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, PR China. Electronic address: Xiaoei.2005@yahoo.com.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, PR China. Electronic address: 451029796@qq.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Xue
authors.affiliation: Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, PR China. Electronic address: apisguo@gmail.com.
authors.forename: Zhanbo
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, PR China. Electronic address: ztapis@263.net.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, PR China. Electronic address: xushufa@caas.cn.
authors.forename: Shufa
authors.initials: S
authors.lastname: Xu
mesh_terms: D001673:Biodegradation, Environmental; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004785:Environmental Pollutants / Q000302:isolation & purification / Q000378:metabolism*; D005818:Genetic Engineering; D007093:Imidazoles / Q000302:isolation & purification / Q000378:metabolism*; D007306:Insecticides / Q000302:isolation & purification / Q000378:metabolism*; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000302:isolation & purification / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D014242:Trichoderma / Q000235:genetics* / Q000378:metabolism*
chemical_list: D004785:Environmental Pollutants; D007093:Imidazoles; D007306:Insecticides; D000073943:Neonicotinoids; D009574:Nitro Compounds; C082359:imidacloprid; D004262:DNA Restriction Enzymes
keywords: Biodegradation; Imidacloprid; Neonicotinoid insecticide; REMI; Trichoderma