title: Blastocyst culture selects for euploid embryos: comparison of blastomere and trophectoderm biopsies.
abstract: Preimplantation genetic diagnosis and screening improves the chances of achieving a viable pregnancy, not only free of undesired single-gene defects but also aneuploidy. In addition, improvements in vitrification provide an efficient means of preserving embryos (blastocysts). By combining trophectoderm biopsy with recent improvements in vitrification methods, only those embryos that have proved themselves viable and potentially more competent are tested. Using array comparative genomic hybridization (aCGH) to assess all 24 chromosomes, aneuploidy rates were compared between day-3 blastomere biopsy and day-5 trophectoderm biopsy. Of those 1603 embryos, 31% were euploid, 62% were aneuploid and 7% not analysable. A significantly larger proportion of embryos were euploid on day-5 biopsy (42%) compared with day-3 biopsy (24%, P<0.0001). The number of euploid embryos per patient was not significantly different. Combining extended culture, trophectoderm biopsy and aneuploidy assessment by aCGH and subsequent vitrification can provide a more efficient means of achieving euploid pregnancies in IVF.
authors.affiliation: New York University Fertility Center, New York University Langone School of Medicine, 660 1st Ave., 5th Floor, New York, NY 10016, United States. Electronic address: alexis.adler@nyumc.org.
authors.forename: Alexis
authors.initials: A
authors.lastname: Adler
authors.affiliation: New York University Fertility Center, New York University Langone School of Medicine, 660 1st Ave., 5th Floor, New York, NY 10016, United States.
authors.forename: Hsaio-Ling
authors.initials: HL
authors.lastname: Lee
authors.affiliation: New York University Fertility Center, New York University Langone School of Medicine, 660 1st Ave., 5th Floor, New York, NY 10016, United States.
authors.forename: David H
authors.initials: DH
authors.lastname: McCulloh
authors.affiliation: New York University Fertility Center, New York University Langone School of Medicine, 660 1st Ave., 5th Floor, New York, NY 10016, United States.
authors.forename: Esmeralda
authors.initials: E
authors.lastname: Ampeloquio
authors.affiliation: New York University Fertility Center, New York University Langone School of Medicine, 660 1st Ave., 5th Floor, New York, NY 10016, United States.
authors.forename: Melicia
authors.initials: M
authors.lastname: Clarke-Williams
authors.affiliation: New York University Fertility Center, New York University Langone School of Medicine, 660 1st Ave., 5th Floor, New York, NY 10016, United States.
authors.forename: Brooke Hodes
authors.initials: BH
authors.lastname: Wertz
authors.affiliation: New York University Fertility Center, New York University Langone School of Medicine, 660 1st Ave., 5th Floor, New York, NY 10016, United States.
authors.forename: James
authors.initials: J
authors.lastname: Grifo
mesh_terms: D000782:Aneuploidy; D001706:Biopsy; D001757:Blastomeres; D055028:Comparative Genomic Hybridization; D046149:Embryo Culture Techniques; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D011247:Pregnancy; D019836:Preimplantation Diagnosis / Q000379:methods
keywords: aneuploidy; array comparative genomic hybridization; blastomere biopsy; preimplantation genetic screening; trophectoderm biopsy; whole-genome amplification