title: Origin of the X1X1X2X2/X1X2Y sex chromosome system of Harttia punctata (Siluriformes, Loricariidae) inferred from chromosome painting and FISH with ribosomal DNA markers.
abstract: Harttia is a genus in the subfamily Loricariinae that accommodates fishes popularly known as armored catfishes. They show extensive karyotypic diversity regarding interspecific numerical/structural variation of the karyotypes, with the presence of the XX/XY1Y2 multiple sex chromosome system, as found in H. carvalhoi. In this context, this study aimed to characterize Harttia punctata chromosomally, for the first time, and to infer the rearrangements that originated the X1X1X2X2/X1X2Y multiple sex chromosome system present in this species. The data obtained in this study, with classical (Giemsa, C-banding and AgNORs) and molecular methodologies (fluorescence in situ hybridization) and chromosome microdissection, indicated that a translocation between distinct acrocentric chromosomes bearing rRNA genes, accompanied by deletions in both chromosomes, might have originated the neo-Y chromosome in this species. The data also suggest that the multiple sex chromosome systems present in H. carvalhoi and H. punctata had an independent origin, evidencing the recurrence of chromosome alterations in species from this genus.
authors.affiliation: Universidade Tecnológica Federal do Paraná, Prolongamento da Rua Cerejeira s/n. Bairro São Luís, Santa Helena, Paraná, CEP: 85892-000, Brazil, danielrblanco@yahoo.com.br.
authors.forename: Daniel Rodrigues
authors.initials: DR
authors.lastname: Blanco
authors.forename: Marcelo Ricardo
authors.initials: MR
authors.lastname: Vicari
authors.forename: Roberto Laridondo
authors.initials: RL
authors.lastname: Lui
authors.forename: Roberto Ferreira
authors.initials: RF
authors.lastname: Artoni
authors.forename: Mara Cristina
authors.initials: MC
authors.lastname: de Almeida
authors.forename: Josiane Baccarin
authors.initials: JB
authors.lastname: Traldi
authors.forename: Vladimir Pavan
authors.initials: VP
authors.lastname: Margarido
authors.forename: Orlando
authors.initials: O
authors.lastname: Moreira-Filho
mesh_terms: D000818:Animals; D005075:Biological Evolution; D002397:Catfishes / Q000145:classification / Q000235:genetics*; D002872:Chromosome Deletion; D020223:Chromosome Painting / Q000379:methods; D020732:Cytogenetic Analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005260:Female; D020459:Genes, rRNA; D014644:Genetic Variation; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D012730:Sex Chromosomes; D014178:Translocation, Genetic
chemical_list: D004275:DNA, Ribosomal