title: Using flow cytometry to detect haemic neoplasia in mussels (Mytilus trossulus) from the Pacific Coast of Southern British Columbia, Canada.
abstract: Flow cytometry was investigated as an alternative to visual haemocytology for potentially higher-throughput and less subjective detection of neoplasia in Mytilus trossulus. In contrast to previous studies of ploidy in the Mytilus spp. complex, distinct tetra- and pentaploidal neoplastic cells were rare and a wide range of aneuploidy peaks from 1.4n to 5.5n were detected for late-stage leukemic animals. There was no correlation between aneuploidy and the number of diseased cells for early and intermediate disease stages. Formation of aneuploidy and neoplasia progression might not be simultaneous, and DNA content analysis using flow cytometry was only useful for detecting late stages of the disease.
authors.affiliation: Chemical and Biological Engineering, University of British Columbia, 2360 East Mall, Vancouver, BC V6T 1Z3, Canada. Electronic address: ekaterina.vassilenko@gmail.com.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Vassilenko
authors.affiliation: Chemical and Biological Engineering, University of British Columbia, 2360 East Mall, Vancouver, BC V6T 1Z3, Canada. Electronic address: sbaldwin@mail.ubc.ca.
authors.forename: Susan A
authors.initials: SA
authors.lastname: Baldwin
mesh_terms: D000818:Animals; D001955:British Columbia; D005434:Flow Cytometry / Q000379:methods*; D006434:Hemocytes / Q000473:pathology*; D049878:Mytilus; D011003:Ploidies
keywords: Biomonitoring; Flow cytometry; Haemic neoplasia; Mussel leukemia; Mytilus trossulus; Ploidy