title: AKR1C3 overexpression may serve as a promising biomarker for prostate cancer progression.
abstract: BACKGROUND
  Aldo-keto reductase family 1 member C3 (AKR1C3) is a key steroidogenic enzyme that is overexpressed in prostate cancer (PCa) and is associated with the development of castration-resistant prostate cancer (CRPC). The aim of this study was to investigate the correlation between the expression level of AKR1C3 and the progression of PCa.
  
  
  METHODS
  Sixty human prostate needle biopsy tissue specimens and ten LNCaP xenografts from intact or castrated male mice were included in the study. The relationship between the level of AKR1C3 expression by immunohistochemistry and evaluation factors for PCa progression, including prostate-specific antigen (PSA), Gleason score (GS) and age, were analyzed.
  
  
  RESULTS
  Low immunoreactivity of AKR1C3 was detected in normal prostate epithelium, benign prostatic hyperplasia (BPH) and prostatic intraepithelial neoplasia (PIN). Positive staining was gradually increased with an elevated GS in PCa epithelium and LNCaP xenografts in mice after castration. The Spearman's r values (rs) of AKR1C3 to GS and PSA levels were 0.396 (P = 0.025) and -0.377 (P = 0.036), respectively, in PCa biopsies. The rs of AKR1C3 to age was 0.76 (P = 0.011). No statistically significant difference was found with other variables.
  
  
  CONCLUSIONS
  Our study suggests that the level of AKR.1C3 expression is positively correlated with an elevated GS, indicating that AKR1C3 can serve as a promising biomarker for the progression of PCa.
  The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/7748245591110149.
authors.forename: Yuantong
authors.initials: Y
authors.lastname: Tian
authors.forename: Lijing
authors.initials: L
authors.lastname: Zhao
authors.forename: Haitao
authors.initials: H
authors.lastname: Zhang
authors.forename: Xichun
authors.initials: X
authors.lastname: Liu
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zhao
authors.forename: Xuejian
authors.initials: X
authors.lastname: Zhao
authors.affiliation: College of Basic Medical Sciences, Jilin University, Changchun 130021, Jilin, China. liyi@jlu.edu.cn.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Li
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000032:analysis / Q000096:biosynthesis*; D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000074425:Aldo-Keto Reductase Family 1 Member C3; D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D018450:Disease Progression; D064593:Heterografts; D006801:Humans; D006911:Hydroxyprostaglandin Dehydrogenases / Q000032:analysis / Q000096:biosynthesis*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D060787:Neoplasm Grading; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor; D015096:3-Hydroxysteroid Dehydrogenases; D006911:Hydroxyprostaglandin Dehydrogenases; C487341:AKR1C3 protein, human; D000074425:Aldo-Keto Reductase Family 1 Member C3