title: Microgravity induces changes in microsome-associated proteins of Arabidopsis seedlings grown on board the international space station.
abstract: The "GENARA A" experiment was designed to monitor global changes in the proteome of membranes of Arabidopsis thaliana seedlings subjected to microgravity on board the International Space Station (ISS). For this purpose, 12-day-old seedlings were grown either in space, in the European Modular Cultivation System (EMCS) under microgravity or on a 1 g centrifuge, or on the ground. Proteins associated to membranes were selectively extracted from microsomes and identified and quantified through LC-MS-MS using a label-free method. Among the 1484 proteins identified and quantified in the 3 conditions mentioned above, 80 membrane-associated proteins were significantly more abundant in seedlings grown under microgravity in space than under 1 g (space and ground) and 69 were less abundant. Clustering of these proteins according to their predicted function indicates that proteins associated to auxin metabolism and trafficking were depleted in the microsomal fraction in µg space conditions, whereas proteins associated to stress responses, defence and metabolism were more abundant in µg than in 1 g indicating that microgravity is perceived by plants as a stressful environment. These results clearly indicate that a global membrane proteomics approach gives a snapshot of the cell status and its signaling activity in response to microgravity and highlight the major processes affected.
authors.affiliation: Laboratoire de Recherches en Sciences Végétales, Université de Toulouse UPS, CNRS UMR5546, Castanet-Tolosan, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Mazars
authors.affiliation: Laboratoire de Recherches en Sciences Végétales, Université de Toulouse UPS, CNRS UMR5546, Castanet-Tolosan, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Brière
authors.affiliation: Laboratoire de Recherches en Sciences Végétales, Université de Toulouse UPS, CNRS UMR5546, Castanet-Tolosan, France.
authors.forename: Sabine
authors.initials: S
authors.lastname: Grat
authors.affiliation: Institut de Pharmacologie et de Biologie Structurale IPBS CNRS, Fédération de Recherche 3450 Agrobiosciences Interactions et Biodiversités Plateforme Protéomique Génopole Toulouse Midi Pyrénées, Toulouse, France.
authors.forename: Carole
authors.initials: C
authors.lastname: Pichereaux
authors.affiliation: Institut de Pharmacologie et de Biologie Structurale IPBS CNRS, Fédération de Recherche 3450 Agrobiosciences Interactions et Biodiversités Plateforme Protéomique Génopole Toulouse Midi Pyrénées, Toulouse, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Rossignol
authors.affiliation: GSBMS, Université de Toulouse, Toulouse, France.
authors.forename: Veronica
authors.initials: V
authors.lastname: Pereda-Loth
authors.affiliation: GSBMS, Université de Toulouse, Toulouse, France.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Eche
authors.affiliation: UR5-PCMP-EAC 7180 CNRS, Université Pierre et Marie Curie-Sorbonne Universités, Paris, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Boucheron-Dubuisson
authors.affiliation: UR5-PCMP-EAC 7180 CNRS, Université Pierre et Marie Curie-Sorbonne Universités, Paris, France.
authors.forename: Isabel
authors.initials: I
authors.lastname: Le Disquet
authors.affiliation: Centro de Investigaciones Biologicas CSIC, Madrid, Spain.
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Medina
authors.affiliation: Laboratoire de Recherches en Sciences Végétales, Université de Toulouse UPS, CNRS UMR5546, Castanet-Tolosan, France.
authors.forename: Annick
authors.initials: A
authors.lastname: Graziana
authors.affiliation: UR5-PCMP-EAC 7180 CNRS, Université Pierre et Marie Curie-Sorbonne Universités, Paris, France.
authors.forename: Eugénie
authors.initials: E
authors.lastname: Carnero-Diaz
mesh_terms: D017360:Arabidopsis / Q000254:growth & development* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism*; D010641:Phenotype; D021381:Protein Transport; D040901:Proteomics; D036226:Seedlings / Q000254:growth & development; D013026:Space Flight; D014893:Weightlessness
chemical_list: D029681:Arabidopsis Proteins; D008565:Membrane Proteins