title: In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes.
abstract: Dendritic cells (DC) are antigen-presenting cells uniquely capable of priming naïve T cells and cross-presenting antigens, and they determine the type of immune response elicited against an antigen. TAT peptide (TATp), is an amphipathic, arginine-rich, cationic peptide that promotes penetration and translocation of various molecules and nanoparticles into cells. TATp-liposomes (TATp-L) used for DC transfection were prepared using TATp derivatized with a lipid-terminated polymer capable of anchoring in the liposomal membrane. Here, we show that the addition of TATp to DNA-loaded liposomes increased the uptake of DNA in DC. DNA-loaded TATp-L increased the in vitro transfection efficiency in DC cultures as evidenced by a higher expression of the enhanced green fluorescent protein and bovine herpes virus type 1 glycoprotein D (gD). The de novo synthesized gD protein was immunologically stimulating when transfections were performed with TATp-L, as indicated by the secretion of interleukin 6.
authors.affiliation: Virology Institute, Center for Research in Veterinary and Agronomic Sciences, National Institute for Agricultural Technology (INTA), Hurlingham, BA, Argentina ; National Council for Scientific and Technical Research (CONICET), Autonomous City of Buenos Aires, Argentina ; Center for Pharmaceutical Biotechnology and Nanomedicine, Northeastern University, Boston, MA, USA.
authors.forename: Juan Sebastián
authors.initials: JS
authors.lastname: Pappalardo
authors.affiliation: National Council for Scientific and Technical Research (CONICET), Autonomous City of Buenos Aires, Argentina.
authors.forename: Cecilia A
authors.initials: CA
authors.lastname: Langellotti
authors.affiliation: Virology Institute, Center for Research in Veterinary and Agronomic Sciences, National Institute for Agricultural Technology (INTA), Hurlingham, BA, Argentina.
authors.forename: Sebastián
authors.initials: S
authors.lastname: Di Giacomo
authors.affiliation: Virology Institute, Center for Research in Veterinary and Agronomic Sciences, National Institute for Agricultural Technology (INTA), Hurlingham, BA, Argentina.
authors.forename: Valeria
authors.initials: V
authors.lastname: Olivera
authors.affiliation: National Council for Scientific and Technical Research (CONICET), Autonomous City of Buenos Aires, Argentina.
authors.forename: Valeria
authors.initials: V
authors.lastname: Quattrocchi
authors.affiliation: Virology Institute, Center for Research in Veterinary and Agronomic Sciences, National Institute for Agricultural Technology (INTA), Hurlingham, BA, Argentina ; National Council for Scientific and Technical Research (CONICET), Autonomous City of Buenos Aires, Argentina.
authors.forename: Patricia I
authors.initials: PI
authors.lastname: Zamorano
authors.affiliation: Center for Pharmaceutical Biotechnology and Nanomedicine, Northeastern University, Boston, MA, USA.
authors.forename: William C
authors.initials: WC
authors.lastname: Hartner
authors.affiliation: Center for Pharmaceutical Biotechnology and Nanomedicine, Northeastern University, Boston, MA, USA.
authors.forename: Tatyana S
authors.initials: TS
authors.lastname: Levchenko
authors.affiliation: Center for Pharmaceutical Biotechnology and Nanomedicine, Northeastern University, Boston, MA, USA.
authors.forename: Vladimir P
authors.initials: VP
authors.lastname: Torchilin
mesh_terms: D000818:Animals; D002417:Cattle; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D005260:Female; D049452:Green Fluorescent Proteins / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis; D008081:Liposomes / Q000008:administration & dosage* / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D050997:Nanomedicine; D010446:Peptide Fragments / Q000008:administration & dosage* / Q000737:chemistry*; D014162:Transfection / Q000379:methods*; D014764:Viral Proteins / Q000235:genetics; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000008:administration & dosage* / Q000737:chemistry*
chemical_list: D015850:Interleukin-6; D008081:Liposomes; D010446:Peptide Fragments; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins; C485184:enhanced green fluorescent protein; D054322:tat Gene Products, Human Immunodeficiency Virus; C497137:tat peptide (47-57), Human immunodeficiency virus 1; D049452:Green Fluorescent Proteins
keywords: TAT peptide; bovine herpes virus 1 glycoprotein D; dendritic cell transfection; green fluorescent protein; interleukin 6; liposomes