title: Evolution of genetic polymorphisms of Plasmodium falciparum merozoite surface protein (PfMSP) in Thailand.
abstract: Plasmodium falciparum malaria is a major public health problem in Thailand due to the emergence of multidrug resistance. The understanding of genetic diversity of malaria parasites is essential for developing effective drugs and vaccines. The genetic diversity of the merozoite surface protein-1 (PfMSP-1) and merozoite surface protein-2 (PfMSP-2) genes was investigated in a total of 145 P. falciparum isolates collected from Mae Sot District, Tak Province, Thailand during 3 different periods (1997-1999, 2005-2007, and 2009-2010). Analysis of genetic polymorphisms was performed to track the evolution of genetic change of P. falciparum using PCR. Both individual genes and their combination patterns showed marked genetic diversity during the 3 study periods. The results strongly support that P. falciparum isolates in Thailand are markedly diverse and patterns changed with time. These 2 polymorphic genes could be used as molecular markers to detect multiple clone infections and differentiate recrudescence from reinfection in P. falciparum isolates in Thailand.
authors.affiliation: Faculty of Allied Health Sciences, Thammasat University, Pathumthani, 12121, Thailand.
authors.forename: Jiraporn
authors.initials: J
authors.lastname: Kuesap
authors.affiliation: Faculty of Allied Health Sciences, Thammasat University, Pathumthani, 12121, Thailand.
authors.forename: Wanna
authors.initials: W
authors.lastname: Chaijaroenkul
authors.affiliation: Faculty of Allied Health Sciences, Thammasat University, Pathumthani, 12121, Thailand.
authors.forename: Kanchanok
authors.initials: K
authors.lastname: Ketprathum
authors.affiliation: Faculty of Allied Health Sciences, Thammasat University, Pathumthani, 12121, Thailand.
authors.forename: Puntanat
authors.initials: P
authors.lastname: Tattiyapong
authors.affiliation: Chulabhorn International College of Medicine, Thammasat University, Pathumthani, 12121, Thailand.
authors.forename: Kesara
authors.initials: K
authors.lastname: Na-Bangchang
mesh_terms: D000953:Antigens, Protozoan / Q000235:genetics*; D016054:DNA, Protozoan / Q000235:genetics; D019143:Evolution, Molecular; D006801:Humans; D016778:Malaria, Falciparum / Q000469:parasitology; D020066:Merozoite Surface Protein 1 / Q000235:genetics*; D010963:Plasmodium falciparum / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D015800:Protozoan Proteins / Q000235:genetics*; D013785:Thailand
chemical_list: D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D020066:Merozoite Surface Protein 1; D015800:Protozoan Proteins; C065974:merozoite surface protein 2, Plasmodium
keywords: Plasmodium falciparum; Thailand; genetic polymorphism; merozoite surface protien