title: [Immunogenic activity of a conjugate of synthetic hexasaccharide related to a streptococcus pneumoniae serotype 14 capsule polysaccharide chain fragment].
abstract: OBJECTIVE
  Study antigenic and immunogenic activity of a conjugate of synthetic hexasaccharide related to a S. pneumoniae serotype 14 capsule polysaccharide chain fragment with bovine serum albumin (BSA).
  
  
  METHODS
  Synthetic glucoconjugate based on BSA protein carrier and hexasaccharide ligand reflecting capsule polysaccharide chain fragment was obtained by using squarate method. Natural polysaccharide-protein complex from S. pneumoniae serotype 14 strain was obtained from cultural fluid supernatant by acetone precipitation. IgG titer against hexasaccharide/capsule polysaccharide was determined in antimicrobial sera and sera of mice immunized with glucoconjugate by EIA method.
  
  
  RESULTS
  Immunogenic activity ofglucoconjugate based on BSA protein carriers and synthetic hexasaccharide reflecting S. pneumoniae serotype 14 capsule protein chain fragment was established. After 2 immunizations antibodies against hexasaccharide ligand and BSA were determined in blood sera of mice. Antibody titers against hexasaccharide exceeded the level in intact mice by 4.2 times. BSA in the conjugate did not have effect on production of antibodies against hexasaccharide.
  
  
  CONCLUSIONS
  The developed experimental test-system based on synthetic glucoconjugate is useful for evaluation of level of antibodies against S. pneumoniae serotype 14 in infected and, probably, carriers of bacteria.
authors.forename: E A
authors.initials: EA
authors.lastname: Kurbatova
authors.forename: D S
authors.initials: DS
authors.lastname: Vorob'ev
authors.forename: N B
authors.initials: NB
authors.lastname: Egorova
authors.forename: I B
authors.initials: IB
authors.lastname: Semenova
authors.forename: E V
authors.initials: EV
authors.lastname: Sukhova
authors.forename: D V
authors.initials: DV
authors.lastname: Iashunskiĭ
authors.forename: Iu E
authors.initials: IuE
authors.lastname: Tsvetkov
authors.forename: N É
authors.initials: NÉ
authors.lastname: Nifant'ev
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D016667:Bacterial Capsules / Q000737:chemistry / Q000276:immunology; D002417:Cattle; D051379:Mice; D008807:Mice, Inbred BALB C; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology; D011135:Polysaccharides, Bacterial / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology; D013296:Streptococcus pneumoniae / Q000737:chemistry / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; D012710:Serum Albumin, Bovine