title: Glycan-functionalized fluorescent chitin nanocrystals for biorecognition applications.
abstract: A new platform based on chitin nanocrystals has been developed for biorecognition applications. TEMPO-oxidized chitin nanocrystals (TCNs) were labeled with a fluorescent imidazoisoquinolinone dye, and simultaneously conjugated with carbohydrate ligands, resulting in dually functionalized TCNs. The biorecognition properties of the nanocrystals were probed with lectins and bacteria, resulting in selective interactions with their corresponding cognate carbohydrate-binding proteins, as visualized by optical, fluorescence, STEM, and TEM imaging. This represents a new approach to multifunctional nanomaterials based on naturally occurring polymers, holding high potential for biomedical applications.
authors.affiliation: Department of Chemistry, KTH - Royal Institute of Technology , S-10044 Stockholm, Sweden.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhou
authors.forename: Núria
authors.initials: N
authors.lastname: Butchosa
authors.forename: H Surangi N
authors.initials: HS
authors.lastname: Jayawardena
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
authors.forename: Mingdi
authors.initials: M
authors.lastname: Yan
authors.forename: Olof
authors.initials: O
authors.lastname: Ramström
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002686:Chitin / Q000737:chemistry*; D003208:Concanavalin A / Q000032:analysis* / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry / Q000302:isolation & purification*; D005456:Fluorescent Dyes / Q000737:chemistry*; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D037121:Plant Lectins / Q000032:analysis* / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D030262:Soybean Proteins / Q000032:analysis* / Q000737:chemistry; D013499:Surface Properties; D010835:Phytohemagglutinins
chemical_list: D002686:Chitin; D003208:Concanavalin A; D005456:Fluorescent Dyes; D037121:Plant Lectins; D011134:Polysaccharides; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins