title: Identification of metabolic pathways expressed by Pichia anomala Kh6 in the presence of the pathogen Botrytis cinerea on apple: new possible targets for biocontrol improvement.
abstract: Yeast Pichia anomala strain Kh6 Kurtzman (Saccharomycetales: Endomycetaceae) exhibits biological control properties that provide an alternative to the chemical fungicides currently used by fruit or vegetable producers against main post-harvest pathogens, such as Botrytis cinerea (Helotiales: Sclerotiniaceae). Using an in situ model that takes into account interactions between organisms and a proteomic approach, we aimed to identify P. anomala metabolic pathways influenced by the presence of B. cinerea. A total of 105 and 60 P. anomala proteins were differentially represented in the exponential and stationary growth phases, respectively. In the exponential phase and in the presence of B. cinerea, the pentose phosphate pathway seems to be enhanced and would provide P. anomala with the needed nucleic acids and energy for the wound colonisation. In the stationary phase, P. anomala would use alcoholic fermentation both in the absence and presence of the pathogen. These results would suggest that the competitive colonisation of apple wounds could be implicated in the mode of action of P. anomala against B. cinerea.
authors.affiliation: Plant Pathology Unit, Gembloux Agro-Bio Tech/University of Liège, Gembloux, Belgium.
authors.forename: Anthony
authors.initials: A
authors.lastname: Kwasiborski
authors.affiliation: Plant Pathology Unit, Gembloux Agro-Bio Tech/University of Liège, Gembloux, Belgium.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Bajji
authors.affiliation: Proteomics Platform, Department of Environment and Agrobiotechnologies/Centre de Recherche Public Gabriel Lippmann, Belvaux, Luxemburg.
authors.forename: Jenny
authors.initials: J
authors.lastname: Renaut
authors.affiliation: Plant Biology Unit, Gembloux Agro-Bio Tech/University of Liège, Gembloux, Belgium.
authors.forename: Pierre
authors.initials: P
authors.lastname: Delaplace
authors.affiliation: Plant Pathology Unit, Gembloux Agro-Bio Tech/University of Liège, Gembloux, Belgium.
authors.forename: M Haissam
authors.initials: MH
authors.lastname: Jijakli
mesh_terms: D020171:Botrytis / Q000502:physiology*; D005656:Fungal Proteins / Q000378:metabolism; D027845:Malus / Q000382:microbiology*; D053858:Metabolic Networks and Pathways; D010427:Pentose Phosphate Pathway; D010572:Pest Control, Biological; D010843:Pichia / Q000254:growth & development / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D020543:Proteome