title: Structural studies of the lipopolysaccharide from the fish pathogen Aeromonas veronii strain Bs19, serotype O16.
abstract: Chemical analyses, mass spectrometry, and NMR spectroscopy were applied to study the structure of the lipopolysaccharide (LPS) isolated from Aeromonas veronii strain Bs19, serotype O16. ESI-MS revealed that the most abundant LPS glycoforms have tetra-acylated or hexa-acylated lipid A species, consisting of a bisphosphorylated GlcN disaccharide with an AraN residue as a non-stoichiometric substituent, and a core oligosaccharide composed of Hep₅Hex₃HexN₁Kdo₁P₁. Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→. This composition was confirmed by mass spectrometry. The charge-deconvoluted ESI FT-ICR MS recorded for the LPS preparations identified mass peaks of SR- and R-form LPS species, that differed by Δm = 698.27 u, a value corresponding to the calculated molecular mass of one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H₂O). Moreover, unspecific fragmentation spectra confirmed the sequence of the sugar residues in the OPS and allowed to assume that the elucidated structure also represented the biological repeating unit.
authors.affiliation: Department of Genetics and Microbiology, M. Curie-Sklodowska University, Akademicka 19, Lublin 20-033, Poland. aturska@hektor.umcs.lublin.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Turska-Szewczuk
authors.affiliation: Division of Structural Biochemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee 4a/c, Borstel D-23845, Germany. kduda@fz-borstel.de.
authors.forename: Katarzyna A
authors.initials: KA
authors.lastname: Duda
authors.affiliation: Division of Bioanalytical Chemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee 10, Borstel D-23845, Germany. dschwudke@fz-borstel.de.
authors.forename: Dominik
authors.initials: D
authors.lastname: Schwudke
authors.affiliation: Department of Fish Diseases, National Veterinary Research Institute, Partyzantow 57, Pulawy 24-100, Poland. a.pekala@piwet.pulawy.pl.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Pekala
authors.affiliation: Department of Fish Diseases, National Veterinary Research Institute, Partyzantow 57, Pulawy 24-100, Poland. koala@piwet.pulawy.pl.
authors.forename: Alicja
authors.initials: A
authors.lastname: Kozinska
authors.affiliation: Division of Structural Biochemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee 4a/c, Borstel D-23845, Germany. oholst@fz-borstel.de.
authors.forename: Otto
authors.initials: O
authors.lastname: Holst
mesh_terms: D000333:Aeromonas / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D000465:Algorithms; D000818:Animals; D002240:Carbohydrate Sequence; D002347:Carps; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D005393:Fish Diseases / Q000382:microbiology*; D005399:Fishes; D008401:Gas Chromatography-Mass Spectrometry; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D016622:Silver Staining; D012883:Skin Ulcer / Q000382:microbiology / Q000662:veterinary; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D003470:Culture Media; D008070:Lipopolysaccharides