title: One-pot hydrazide-based native chemical ligation for efficient chemical synthesis and structure determination of toxin Mambalgin-1.
abstract: An efficient one-pot chemical synthesis of snake venom toxin Mambalgin-1 was achieved using an azide-switch strategy combined with hydrazide-based native chemical ligation. Synthetic Mambalgin-1 exhibited a well-defined structure after sequential folding in vitro. NMR spectroscopy revealed a three-finger toxin family structure, and the synthetic toxin inhibited human acid-sensing ion channel 1a.
authors.affiliation: School of Life Sciences, University of Science and Technology of China, and High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei 230026, China. zlhustc@ustc.edu.cn cltian@ustc.edu.cn.
authors.forename: Man
authors.initials: M
authors.lastname: Pan
authors.forename: Yao
authors.initials: Y
authors.lastname: He
authors.forename: Ming
authors.initials: M
authors.lastname: Wen
authors.forename: Fangming
authors.initials: F
authors.lastname: Wu
authors.forename: Demeng
authors.initials: D
authors.lastname: Sun
authors.forename: Sijian
authors.initials: S
authors.lastname: Li
authors.forename: Longhua
authors.initials: L
authors.lastname: Zhang
authors.forename: Yiming
authors.initials: Y
authors.lastname: Li
authors.forename: Changlin
authors.initials: C
authors.lastname: Tian
mesh_terms: D062565:Acid Sensing Ion Channels / Q000187:drug effects; D001386:Azides / Q000737:chemistry*; D004546:Elapid Venoms / Q000138:chemical synthesis* / Q000737:chemistry / Q000633:toxicity; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000633:toxicity; D011487:Protein Conformation
chemical_list: C568266:ASIC1 protein, human; D062565:Acid Sensing Ion Channels; D001386:Azides; D004546:Elapid Venoms; D010455:Peptides; C576673:mambalgin-1, Dendroaspis polylepis