title: Evaluation of Abbott RealTime CT/NG assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in cervical swabs from female sex workers in China.
abstract: BACKGROUND
  To evaluate the performance of the Abbott RealTime CT/NG assay for detection of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) among female sex workers (FSWs) in China.
  
  
  METHODS
  Cervical swabs from 997 participants were blindly detected by the Abbott RealTime CT/NG assay on the automated m2000 molecular platform and Roche Cobas Amplicor CT/NG assay. Discrepant analysis were confirmed by the Qiagen care CT PCR assay. The sample was defined as candidate nvCT-positive if it was CT positive in the Abbott m2000 assay, but CT negative in the other two assays.
  
  
  RESULTS
  25 specimens that were discordant for CT and 26 specimens that were discordant for NG between the two assays were resolved by Qiagen care CT & NG PCR assays. The sensitivity and specificity, respectively, for Abbott m2000 assay were 92.59% and 100% for CT and 95.45% and 99.90% for NG. The positive predictive value (PPV) and negative predictive value (NPV) of Abbott m2000 assay were100% and 98.52% for CT and 95.5% and 99.90% for NG, respectively. No candidate new-variant CT(nvCT)specimens were identified.
  
  
  CONCLUSIONS
  Abbott RealTime CT/NG assay were more specify for CT and NG detection, however, its sensitivity for CT and NG were a little bit lower than Roche Cobas Amplicor CT/NG assay. Abbott RealTime CT/NG assay had higher PPV for NG detection than Roche Cobas Amplicor CT/NG assay; it would be more suitable for screening for population with low-prevalence NG. There is currently no evidence that nvCT is present in FSWs in China.
authors.affiliation: National Center for STD Control, China Chinese CDC, and Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Han
authors.affiliation: National Center for STD Control, China Chinese CDC, and Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, China.
authors.forename: Yue-ping
authors.initials: YP
authors.lastname: Yin
authors.affiliation: National Center for STD Control, China Chinese CDC, and Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, China.
authors.forename: Mei-qin
authors.initials: MQ
authors.lastname: Shi
authors.affiliation: National Center for STD Control, China Chinese CDC, and Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, China.
authors.forename: Bing-jie
authors.initials: BJ
authors.lastname: Zheng
authors.affiliation: National Center for STD Control, China Chinese CDC, and Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, China.
authors.forename: Ming-ying
authors.initials: MY
authors.lastname: Zhong
authors.affiliation: National Center for STD Control, China Chinese CDC, and Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Jiang
authors.affiliation: National Center for STD Control, China Chinese CDC, and Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, China.
authors.forename: Shao-chun
authors.initials: SC
authors.lastname: Chen
authors.affiliation: National Center for STD Control, China Chinese CDC, and Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, China.
authors.forename: Xiang-sheng
authors.initials: XS
authors.lastname: Chen
mesh_terms: D000328:Adult; D002681:China; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D006069:Gonorrhea / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D009344:Neisseria gonorrhoeae / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D060386:Sex Workers; D014626:Vaginal Smears; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial