title: PEGylation of αα-Hb using succinimidyl propionic acid PEG 5K: Conjugation chemistry and PEG shell structure dictate respectively the oxygen affinity and resuscitation fluid like properties of PEG αα-Hbs.
abstract: PEGylation of intramolecularly crosslinked Hb has been studied here to overcome the limitation of dissociation of Hb tetramers. New hexa and deca PEGylated low oxygen affinity PEG-ααHbs have been generated. Influence of PEG conjugation chemistry and the PEG shell structure on the functional properties as well as PEGylation induced plasma expander like properties of the protein has been delineated. The results have established that in the design of PEG-Hbs as oxygen therapeutics, the influence of conjugation chemistry and the PEG shell structure on the oxygen affinity of Hb needs to be optimized independently besides optimizing the PEG shell structure for inducing resuscitation fluid like properties.
authors.affiliation: Hematology Division, Department of Medicine, Albert Einstein College of Medicine of Yeshiva University , Bronx, NY , USA.
authors.forename: Fantao
authors.initials: F
authors.lastname: Meng
authors.forename: Amy G
authors.initials: AG
authors.lastname: Tsai
authors.forename: Marcos
authors.initials: M
authors.lastname: Intaglietta
authors.forename: Seetharama A
authors.initials: SA
authors.lastname: Acharya
mesh_terms: D001802:Blood Substitutes / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D010100:Oxygen / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D001802:Blood Substitutes; D006454:Hemoglobins; D011092:Polyethylene Glycols; D010100:Oxygen; C000595209:polyethylene glycol 1000
keywords: PEG shell; PEGylated hemoglobin; PEGylation; blood substitute; oxygen affinity; plasma expander