title: The protein expression profile of meningioma cells is associated with distinct cytogenetic tumour subgroups.
abstract: OBJECTIVE
  Limited information exists about the impact of cytogenetic alterations on the protein expression profiles of individual meningioma cells and their association with the clinicohistopathological characteristics of the disease. The aim of this study is to investigate the potential association between the immunophenotypic profile of single meningioma cells and the most relevant features of the tumour.
  
  
  METHODS
  Multiparameter flow cytometry (MFC) was used to evaluate the immunophenotypic profile of tumour cells (n = 51 patients) and the Affymetrix U133A chip was applied for the analysis of the gene expression profile (n = 40) of meningioma samples, cytogenetically characterized by interphase fluorescence in situ hybridization.
  
  
  RESULTS
  Overall, a close association between the pattern of protein expression and the cytogenetic profile of tumour cells was found. Thus, diploid tumours displayed higher levels of expression of the CD55 complement regulatory protein, tumours carrying isolated monosomy 22/del(22q) showed greater levels of bcl2 and PDGFRβ and meningiomas carrying complex karyotypes displayed a greater proliferation index and decreased expression of the CD13 ectoenzyme, the CD9 and CD81 tetraspanins, and the Her2/neu growth factor receptor. From the clinical point of view, higher expression of CD53 and CD44 was associated with a poorer outcome.
  
  
  CONCLUSIONS
  Here we show that the protein expression profile of individual meningioma cells is closely associated with tumour cytogenetics, which may reflect the involvement of different signalling pathways in the distinct cytogenetic subgroups of meningiomas, with specific immunophenotypic profiles also translating into a different tumour clinical behaviour.
authors.affiliation: Center for Cancer Research (CIC-IBMCC; CSIC/USAL), IBSAL, Cytometry service (NUCLEUS), Department of Medicine, University of Salamanca, Salamanca, Spain; Center for Neurosciences and Cell Biology and Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal.
authors.forename: Patrícia Henriques
authors.initials: PH
authors.lastname: Domingues
authors.forename: Cristina
authors.initials: C
authors.lastname: Teodósio
authors.forename: Álvaro
authors.initials: Á
authors.lastname: Otero
authors.forename: Pablo
authors.initials: P
authors.lastname: Sousa
authors.forename: Jesus Maria
authors.initials: JM
authors.lastname: Gonçalves
authors.forename: Ana Belen
authors.initials: AB
authors.lastname: Nieto
authors.forename: Maria Celeste
authors.initials: MC
authors.lastname: Lopes
authors.forename: Catarina
authors.initials: C
authors.lastname: de Oliveira
authors.forename: Alberto
authors.initials: A
authors.lastname: Orfao
authors.forename: Maria Dolores
authors.initials: MD
authors.lastname: Tabernero
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D002469:Cell Separation; D020732:Cytogenetic Analysis; D005260:Female; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008577:Meningeal Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D008579:Meningioma / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor
keywords: CD44; CD53; iFISH; meningioma; multiparameter flow cytometry; protein expression profile