title: Nasopharyngeal microbiota in healthy children and pneumonia patients.
abstract: Our study is the first to compare the nasopharyngeal microbiota of pediatric pneumonia patients and control children by 454 pyrosequencing. A distinct microbiota was associated with different pneumonia etiologies. Viral pneumonia was associated with a high abundance of the operational taxonomic unit (OTU) corresponding to Moraxella lacunata. Patients with nonviral pneumonia showed high abundances of OTUs of three typical bacterial pathogens, Streptococcus pneumoniae complex, Haemophilus influenzae complex, and Moraxella catarrhalis. Patients classified as having no definitive etiology harbored microbiota particularly enriched in the H. influenzae complex. We did not observe a commensal taxon specifically associated with health. The microbiota of the healthy nasopharynx was more diverse and contained a wider range of less abundant taxa.
authors.affiliation: Nestlé Research Center, Lausanne, Switzerland.
authors.forename: Olga
authors.initials: O
authors.lastname: Sakwinska
authors.forename: Viktoria
authors.initials: V
authors.lastname: Bastic Schmid
authors.forename: Bernard
authors.initials: B
authors.lastname: Berger
authors.forename: Anne
authors.initials: A
authors.lastname: Bruttin
authors.forename: Kristina
authors.initials: K
authors.lastname: Keitel
authors.forename: Mélissa
authors.initials: M
authors.lastname: Lepage
authors.forename: Deborah
authors.initials: D
authors.lastname: Moine
authors.forename: Catherine
authors.initials: C
authors.lastname: Ngom Bru
authors.forename: Harald
authors.initials: H
authors.lastname: Brüssow
authors.forename: Alain
authors.initials: A
authors.lastname: Gervaix
mesh_terms: D000293:Adolescent; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D006192:Haemophilus Infections / Q000175:diagnosis / Q000382:microbiology; D006193:Haemophilus influenzae / Q000235:genetics; D006801:Humans; D007223:Infant; D064307:Microbiota / Q000235:genetics*; D001936:Moraxella catarrhalis / Q000235:genetics; D045828:Moraxellaceae Infections / Q000175:diagnosis / Q000382:microbiology; D009305:Nasopharynx / Q000382:microbiology* / Q000821:virology*; D011008:Pneumococcal Infections / Q000175:diagnosis / Q000382:microbiology; D011014:Pneumonia / Q000175:diagnosis / Q000382:microbiology* / Q000821:virology*; D011446:Prospective Studies; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000382:microbiology / Q000821:virology; D013296:Streptococcus pneumoniae / Q000235:genetics