title: Ixora coccinea Enhances Cutaneous Wound Healing by Upregulating the Expression of Collagen and Basic Fibroblast Growth Factor.
abstract: Background. Ixora coccinea L. (Rubiaceae) has been documented for traditional use in hypertension, menstrual irregularities, sprain, chronic ulcer, and skin diseases. In the present study, I. coccinea was subjected to in vitro and in vivo wound healing investigation. Methods. Petroleum ether, chloroform, methanol, and water sequential I. coccinea leaves extracts were evaluated for in vitro antioxidant, antimicrobial, and fibroblast proliferation activities. The promising I. coccinea methanol extract (IxME) was screened for in vivo wound healing activity in Wistar rat using circular excision model. Wound contraction measurement, hydroxyproline quantification, and western blot for collagen type III (COL3A1), basic fibroblast growth factor (bFGF), and Smad-2, -3, -4, and -7 was performed with 7-day postoperative wound granulation tissue. Gentamicin sulfate (0.01% w/w) hydrogel was used as reference standard. Results. IxME showed the potent antimicrobial, antioxidant activities, with significant fibroblast proliferation inducing activity, as compared to all other extracts. In vivo study confirmed the wound healing accelerating potential of IxME, as evidenced by faster wound contraction, higher hydroxyproline content, and improved histopathology of granulation tissue. Western blot analysis revealed that the topical application of I. coccinea methanol extract stimulates the fibroblast growth factor and Smad mediated collagen production in wound tissue.
authors.affiliation: Division of Pharmaceutical Technology, Defence Research Laboratory, DRDO, Tezpur, Assam 784001, India ; Department of Pharmaceutical Sciences, Birla Institute of Technology, Mesra, Ranchi, Jharkhand 835215, India.
authors.forename: Aadesh
authors.initials: A
authors.lastname: Upadhyay
authors.affiliation: Division of Pharmaceutical Technology, Defence Research Laboratory, DRDO, Tezpur, Assam 784001, India.
authors.forename: Pronobesh
authors.initials: P
authors.lastname: Chattopadhyay
authors.affiliation: Division of Pharmaceutical Technology, Defence Research Laboratory, DRDO, Tezpur, Assam 784001, India.
authors.forename: Danswrang
authors.initials: D
authors.lastname: Goyary
authors.affiliation: Department of Pharmaceutical Sciences, Birla Institute of Technology, Mesra, Ranchi, Jharkhand 835215, India.
authors.forename: Papiya
authors.initials: P
authors.lastname: Mitra Mazumder
authors.affiliation: Division of Pharmaceutical Technology, Defence Research Laboratory, DRDO, Tezpur, Assam 784001, India.
authors.forename: Vijay
authors.initials: V
authors.lastname: Veer