title: [Preparation and application of polyclonal antibody against chicken high mobility group box 1 protein].
abstract: OBJECTIVE
  To prepare an affinity purified polyclonal antibody against chicken high mobility group box 1 protein (chHMGB1) and put it in a practical application.
  
  
  METHODS
  The full-length chHMGB1 cDNA was amplified by reverse transcription PCR (RT-PCR). The specific peptides was selected by sequence alignment and conjugated with KLH. Then it was used to immunize the New Zealand rabbits to obtain the anti-serum. The polyclonal antibody was purified by protein A column and peptide-conjugated Seprose4B sequentially. ELISA, Western blotting, indirect fluorescence assay (IFA) and immunohistochemistry were used for the identification and application of the affinity purified polyclonal antibody.
  
  
  RESULTS
  These examinations demonstrated the successful generation of the affinity purified polyclonal antibody against chHMGB1 protein and the specificity of the polyclonal antibody to both the recombinant protein and the natural chHMGB1 protein.
  
  
  CONCLUSIONS
  The affinity purified polyclonal antibody was successfully prepared.
authors.affiliation: Jiangsu Key Laboratory of Preventive Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
authors.forename: Na
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Jiangsu Key Laboratory of Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Qian
authors.affiliation: Jiangsu Key Laboratory of Preventive Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
authors.forename: Mingyue
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Jiangsu Key Laboratory of Preventive Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
authors.forename: Aijun
authors.initials: A
authors.lastname: Gao
authors.affiliation: Jiangsu Key Laboratory of Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Shao
authors.affiliation: Jiangsu Key Laboratory of Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Jin
authors.affiliation: Jiangsu Key Laboratory of Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China.
authors.forename: Aijian
authors.initials: A
authors.lastname: Qin
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000915:Antibody Affinity; D000918:Antibody Specificity; D015153:Blotting, Western; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D024243:HMGB1 Protein / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D007150:Immunohistochemistry; D011817:Rabbits
chemical_list: D000906:Antibodies; D024243:HMGB1 Protein