title: The C-terminal repeat domains of nsP3 from the Old World alphaviruses bind directly to G3BP.
abstract: The Old World alphaviruses block stress granule assembly by sequestration of RasGAP SH3-domain binding protein (G3BP). Here, we show that the proline-rich sequences in the hypervariable domain of nonstructural protein 3 (nsP3) of both Semliki Forest virus and Chikungunya virus were dispensable for binding to G3BP. nsP3 variants with or without this domain colocalized with G3BP. Furthermore, we show that the C-terminal repeat motifs of nsP3 were sufficient for G3BP binding.
authors.affiliation: Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Marc D
authors.initials: MD
authors.lastname: Panas
authors.forename: Tero
authors.initials: T
authors.lastname: Ahola
authors.forename: Gerald M
authors.initials: GM
authors.lastname: McInerney
mesh_terms: D002646:Chikungunya virus / Q000502:physiology*; D054884:Host-Pathogen Interactions; D012672:Semliki forest virus / Q000502:physiology*; D017361:Viral Nonstructural Proteins / Q000378:metabolism*
chemical_list: D017361:Viral Nonstructural Proteins