title: [Expression of nucleolin in pressure overload-induced cardiac hypertrophy rats].
abstract: OBJECTIVE
  To detect the expression of nucleolin in cardiac hypertrophy rats induced by pressure overload.
  
  
  METHODS
  A total of 40 SD rats with body weight 180 g and 220 g were recruited and randomly divided into 2 groups: a transverse aortic constriction (TAC) group and a sham surgery group. Cardiac hypertrophy model was employed by transverse aortic constriction surgery. Then 2 weeks and 4 weeks after the experiment, the heart mass index (HMI), left ventricle mass index (LVMI) were measured. β-MHC mRNA in the heart tissue was detected with RT-PCR. Nucleolin in the heart, brain and kidney was respectively detected with Western blot.
  
  
  RESULTS
  Compared with the sham surgery group, HMI, LVMI in the TAC group increased significantly (P<0.01) 4 weeks after the surgery; the expression of β-MHC mRNA in the heart tissue increased (P<0.05) in the TAC group 4 weeks after the surgery; and the expression of nucleolin protein in the heart tissue of the TAC group was remarkably upregulated (P<0.05) 2 weeks after the surgery, with no change in the brain and kidney tissue between the 2 groups.
  
  
  CONCLUSIONS
  Expression of nucleolin protein has been upregulated in response to pressure overload, which may suggest that nucleolin plays a role in cardiac hypertrophy induced by pressure overload.
authors.affiliation: Clinical Medical Major, Grade 2008,Xiangya School of Medicine, Central South University, Changsha 410078, China.
authors.forename: Simin
authors.initials: S
authors.lastname: Yan
authors.forename: Shuang
authors.initials: S
authors.lastname: Wu
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.forename: Bimei
authors.initials: B
authors.lastname: Jiang
authors.forename: Zizhi
authors.initials: Z
authors.lastname: Tu
authors.forename: Xianzhong
authors.initials: X
authors.lastname: Xiao
mesh_terms: D000818:Animals; D001794:Blood Pressure; D006332:Cardiomegaly / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D012333:RNA, Messenger; D016601:RNA-Binding Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000097796:Nucleolin
chemical_list: D010750:Phosphoproteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D000097796:Nucleolin