title: Long-term manure exposure increases soil bacterial community potential for plasmid uptake.
abstract: Microbial communities derived from soils subject to different agronomic treatments were challenged with three broad host range plasmids, RP4, pIPO2tet and pRO101, via solid surface filter matings to assess their permissiveness. Approximately 1 in 10 000 soil bacterial cells could receive and maintain the plasmids. The community permissiveness increased up to 100% in communities derived from manured soil. While the plasmid transfer frequency was significantly influenced by both the type of plasmid and the agronomic treatment, the diversity of the transconjugal pools was purely plasmid dependent and was dominated by β- and γ-Proteobacteria.
authors.affiliation: Department of Environmental Engineering, Technical University of Denmark, Kgs. Lyngby, Denmark.
authors.forename: Sanin
authors.initials: S
authors.lastname: Musovic
authors.forename: Uli
authors.initials: U
authors.lastname: Klümper
authors.forename: Arnaud
authors.initials: A
authors.lastname: Dechesne
authors.forename: Jakob
authors.initials: J
authors.lastname: Magid
authors.forename: Barth F
authors.initials: BF
authors.lastname: Smets
mesh_terms: D016573:Agrochemicals / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D044822:Biodiversity; D022761:Gene Transfer, Horizontal; D008372:Manure / Q000032:analysis / Q000382:microbiology*; D010957:Plasmids / Q000235:genetics* / Q000378:metabolism; D012988:Soil Microbiology
chemical_list: D016573:Agrochemicals; D008372:Manure