title: Circulating human rotavirus specific CD4 T cells identified with a class II tetramer express the intestinal homing receptors α4β7 and CCR9.
abstract: Using a consensus epitope prediction approach, three rotavirus (RV) peptides that induce cytokine secretion by CD4 T cells from healthy volunteers were identified. The peptides were shown to bind HLA-DRB1*0101 and then used to generate MHC II tetramers. RV specific T cell lines specific for one of the three peptides studied were restricted by MHC class II molecules and contained T cells that bound the tetramer and secreted cytokines upon activation with the peptide. The majority of RV and Flu tetramer(+) CD4 T cells in healthy volunteers expressed markers of antigen experienced T cells, but only RV specific CD4 T cells expressed intestinal homing receptors. CD4 T cells from children that received a RV vaccine, but not placebo recipients, were stained with the RV-VP6 tetramer and also expressed intestinal homing receptors. Circulating RV-specific CD4 T cells represent a unique subset that expresses intestinal homing receptors.
authors.affiliation: Instituto de Genética Humana, Facultad de Medicina, Pontificia Universidad Javeriana, Bogotá, Colombia.
authors.forename: Miguel
authors.initials: M
authors.lastname: Parra
authors.affiliation: Instituto de Genética Humana, Facultad de Medicina, Pontificia Universidad Javeriana, Bogotá, Colombia.
authors.forename: Daniel
authors.initials: D
authors.lastname: Herrera
authors.affiliation: Department of Pathology, University of Massachusetts Medical School, Worcester, MA 01655, USA.
authors.forename: J Mauricio
authors.initials: JM
authors.lastname: Calvo-Calle
authors.affiliation: Department of Pathology, University of Massachusetts Medical School, Worcester, MA 01655, USA.
authors.forename: Lawrence J
authors.initials: LJ
authors.lastname: Stern
authors.affiliation: Facultad de Medicina, Departamento de Microbiología, Universidad Nacional de Colombia, Bogotá, Colombia.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Parra-López
authors.affiliation: Laboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.
authors.forename: Eugene
authors.initials: E
authors.lastname: Butcher
authors.affiliation: Instituto de Genética Humana, Facultad de Medicina, Pontificia Universidad Javeriana, Bogotá, Colombia.
authors.forename: Manuel
authors.initials: M
authors.lastname: Franco
authors.affiliation: Instituto de Genética Humana, Facultad de Medicina, Pontificia Universidad Javeriana, Bogotá, Colombia. Electronic address: jangel@javeriana.edu.co.
authors.forename: Juana
authors.initials: J
authors.lastname: Angel
mesh_terms: D000293:Adolescent; D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002648:Child; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D006801:Humans; D016023:Integrins / Q000235:genetics* / Q000276:immunology; D007422:Intestines / Q000276:immunology* / Q000821:virology; D008297:Male; D054388:Receptors, CCR / Q000235:genetics* / Q000276:immunology; D011991:Receptors, Virus / Q000235:genetics* / Q000276:immunology; D012401:Rotavirus / Q000235:genetics / Q000276:immunology*; D012400:Rotavirus Infections / Q000235:genetics / Q000276:immunology* / Q000821:virology; D013045:Species Specificity; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology; D055815:Young Adult
chemical_list: C119309:CC chemokine receptor 9; D000949:Histocompatibility Antigens Class II; D016023:Integrins; D054388:Receptors, CCR; D011991:Receptors, Virus; D014764:Viral Proteins; C071777:integrin alpha4beta7
keywords: Intestinal homing receptors; MHC class II tetramers; Rotavirus (RV); T cells