title: DWARF3 participates in an SCF complex and associates with DWARF14 to suppress rice shoot branching.
abstract: Strigolactones (SLs) are a novel class of plant hormones that inhibit shoot branching. Currently, two proteins in rice are thought to play crucial roles in SL signal transduction. DWARF14 (D14), an α/β hydrolase, is responsible for SL perception, while DWARF3 (D3), an F-box protein with leucine-rich repeats, is essential for SL signal transduction. However, how these two proteins transmit SL signals to downstream factors remains unclear. Here, we characterized a high-tillering dwarf rice mutant, gsor300097, which is insensitive to GR24, a synthetic analog of SL. Mapping and sequencing analysis showed that gsor300097 is a novel allelic mutant of D3, in which a nonsense mutation truncates the protein from 720 to 527 amino acids. The D3 gene was strongly expressed in root, leaf, shoot base and panicle. Nuclear-localized F-box protein D3 played a role in the SCF complex by interacting with OSK1, OSK5 or OSK20 and OsCullin1. In addition, D3 associated with D14 in a GR24-dependent manner in vivo. Taken together, our findings suggested that D3 assembled into an SCF(D3) complex and associated with D14 to suppress rice shoot branching.
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaThese authors contributed equally to this work.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Zhao
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaThese authors contributed equally to this work.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Minxia
authors.initials: M
authors.lastname: Wang
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Shandong Rice Research Institute, Jinan 250100, China.
authors.forename: Shoujiang
authors.initials: S
authors.lastname: Yuan
authors.affiliation: School of Life Science, Liaocheng University, Liaocheng 252059, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Shandong Rice Research Institute, Jinan 250100, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Yin
authors.affiliation: Shandong Rice Research Institute, Jinan 250100, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Lixiang
authors.initials: L
authors.lastname: Peng
authors.affiliation: School of Life Science, Liaocheng University, Liaocheng 252059, China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Zhang
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Wan
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China lixueyong@caas.cn.
authors.forename: Xueyong
authors.initials: X
authors.lastname: Li
mesh_terms: D000483:Alleles; D044842:Cullin Proteins / Q000235:genetics / Q000378:metabolism; D044783:F-Box Proteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D006867:Hydrolases / Q000235:genetics / Q000378:metabolism; D007783:Lactones / Q000378:metabolism*; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D010641:Phenotype; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000378:metabolism*; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D036226:Seedlings / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques
chemical_list: C100318:Cullin 1; D044842:Cullin Proteins; D044783:F-Box Proteins; D007783:Lactones; D010937:Plant Growth Regulators; D010940:Plant Proteins; C483518:strigol; D006867:Hydrolases
keywords: GR24; High-tillering dwarf; SCF complex; Shoot branching; Strigolactones