title: Enhancement of antitumor immunity using a DNA-based replicon vaccine derived from Semliki Forest virus.
abstract: A DNA-based replicon vaccine derived from Semliki Forest virus, PSVK-shFcG-GM/B7.1 (Fig. 1a) was designed for tumor immunotherapy as previously constructed. The expression of the fusion tumor antigen (survivin and hCGβ-CTP37) and adjuvant molecular protein (Granulocyte-Macrophage Colony-Stimulating Factor/ GM-CSF/B7.1) genes was confirmed by Immunofluorescence assay in vitro, and immunohistochemistry assay in vivo. In this paper, the immunological effect of this vaccine was determined using immunological assays as well as animal models. The results showed that this DNA vaccine induced both humoral and cellular immune responses in C57BL/6 mice after immunization, as evaluated by the ratio of CD4+/CD8+ cells and the release of IFN-γ. Furthermore, the vaccination of C57BL/6 mice with PSVK-shFcG-GM/B7.1 significantly delayed the in vivo growth of tumors in animal models (survivin+ and hCGβ+ murine melanoma, B16) when compared to vaccination with the empty vector or the other control constructs (Fig. 1b). These data indicate that this type of replicative DNA vaccine could be developed as a promising approach for tumor immunotherapy. Meanwhile, these results provide a basis for further study in vaccine pharmacodynamics and pharmacology, and lay a solid foundation for clinical application.
authors.affiliation: Beijing Institute of Basic Medical Sciences, Haidian district, Beijing, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Beijing Institute of Basic Medical Sciences, Haidian district, Beijing, China; National Center for AIDS/STD Control and Prevention, China-CDC, Beijing, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Beijing Institute of Basic Medical Sciences, Haidian district, Beijing, China; Department of Urology, First Affiliated Hospital of General Hospital of PLA, Beijing, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Beijing Institute of Basic Medical Sciences, Haidian district, Beijing, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Beijing Institute of Basic Medical Sciences, Haidian district, Beijing, China.
authors.forename: JinKai
authors.initials: J
authors.lastname: Dong
authors.affiliation: Beijing Institute of Basic Medical Sciences, Haidian district, Beijing, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Gao
authors.affiliation: Beijing Institute of Basic Medical Sciences, Haidian district, Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Beijing Institute of Basic Medical Sciences, Haidian district, Beijing, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
authors.affiliation: Beijing Institute of Basic Medical Sciences, Haidian district, Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Beijing Institute of Basic Medical Sciences, Haidian district, Beijing, China.
authors.forename: Yuanji
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Beijing Institute of Basic Medical Sciences, Haidian district, Beijing, China.
authors.forename: JinQi
authors.initials: J
authors.lastname: Yan
authors.affiliation: Beijing Institute of Basic Medical Sciences, Haidian district, Beijing, China.
authors.forename: JiYun
authors.initials: J
authors.lastname: Yu
mesh_terms: D000818:Animals; D019496:Cancer Vaccines / Q000276:immunology*; D045744:Cell Line, Tumor; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology; D056724:Immunity, Humoral / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D012093:Replicon / Q000276:immunology*; D012672:Semliki forest virus / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D019496:Cancer Vaccines; D019444:Vaccines, DNA; D016178:Granulocyte-Macrophage Colony-Stimulating Factor