title: A quantum dot-based microfluidic multi-window platform for quantifying the biomarkers of breast cancer cells.
abstract: Conventional molecular profiling methods using immunochemical assays have limits in terms of multiplexity and the quantification of biomarkers in investigation of cancer cells. In this paper, we demonstrate a quantum dot (QD)-based microfluidic multiple biomarker quantification (QD-MMBQ) method that enables labeling of more than eight proteins immunochemically on cell blocks within 1 h, in a quantitative manner. An internal reference, β-actin, was used as a loading control to compensate for differences in not only the cell number but also in staining quality among specimens. Furthermore, the microfluidic blocking method exhibited less nonspecific binding of QDs than the conventional static blocking method.
authors.affiliation: Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea. jekyun@kaist.ac.kr.
authors.forename: Seyong
authors.initials: S
authors.lastname: Kwon
authors.forename: Minseok S
authors.initials: MS
authors.lastname: Kim
authors.forename: Eun Sook
authors.initials: ES
authors.lastname: Lee
authors.forename: Jang Sihn
authors.initials: JS
authors.lastname: Sohn
authors.forename: Je-Kyun
authors.initials: JK
authors.lastname: Park
mesh_terms: D066259:Betacellulin; D015415:Biomarkers / Q000032:analysis*; D001943:Breast Neoplasms / Q000175:diagnosis*; D045744:Cell Line, Tumor; D047628:Estrogen Receptor alpha / Q000032:analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis; D019394:Ki-67 Antigen / Q000032:analysis; D044085:Microfluidics / Q000379:methods*; D045663:Quantum Dots; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis; D020893:Receptor, ErbB-3 / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis; D058570:TOR Serine-Threonine Kinases / Q000032:analysis; D016211:Transforming Growth Factor alpha / Q000032:analysis
chemical_list: D066259:Betacellulin; D015415:Biomarkers; D047628:Estrogen Receptor alpha; D036341:Intercellular Signaling Peptides and Proteins; D019394:Ki-67 Antigen; D018719:Erb-b2 Receptor Tyrosine Kinases; D020893:Receptor, ErbB-3; D011980:Receptors, Progesterone; D058570:TOR Serine-Threonine Kinases; D016211:Transforming Growth Factor alpha; C587851:BTC protein, human; C546842:MTOR protein, human; C508053:ERBB2 protein, human; C581292:ERBB3 protein, human