title: A fully synthetic glycopeptide antitumor vaccine based on multiple antigen presentation on a hyperbranched polymer.
abstract: For antitumor vaccines both the selected tumor-associated antigen, as well as the mode of its presentation, affect the immune response. According to the principle of multiple antigen presentation, a tumor-associated MUC1 glycopeptide combined with the immunostimulating T-cell epitope P2 from tetanus toxoid was coupled to a multi-functionalized hyperbranched polyglycerol by "click chemistry". This globular polymeric carrier has a flexible dendrimer-like structure, which allows optimal antigen presentation to the immune system. The resulting fully synthetic vaccine induced strong immune responses in mice and IgG antibodies recognizing human breast-cancer cells.
authors.affiliation: Johannes Gutenberg University Mainz, Institute of Organic Chemistry, Duesbergweg 10-14, 55128 Mainz (Germany).
authors.forename: Markus
authors.initials: M
authors.lastname: Glaffig
authors.forename: Björn
authors.initials: B
authors.lastname: Palitzsch
authors.forename: Sebastian
authors.initials: S
authors.lastname: Hartmann
authors.forename: Christoph
authors.initials: C
authors.lastname: Schüll
authors.forename: Lutz
authors.initials: L
authors.lastname: Nuhn
authors.forename: Bastian
authors.initials: B
authors.lastname: Gerlitzki
authors.forename: Edgar
authors.initials: E
authors.lastname: Schmitt
authors.forename: Holger
authors.initials: H
authors.lastname: Frey
authors.forename: Horst
authors.initials: H
authors.lastname: Kunz
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D017951:Antigen Presentation; D000951:Antigens, Neoplasm / Q000276:immunology*; D001943:Breast Neoplasms / Q000188:drug therapy; D019496:Cancer Vaccines / Q000276:immunology* / Q000627:therapeutic use; D057930:Click Chemistry; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry / Q000276:immunology; D005260:Female; D005990:Glycerol / Q000737:chemistry*; D006020:Glycopeptides / Q000138:chemical synthesis* / Q000276:immunology; D006801:Humans; D061986:MCF-7 Cells; D051379:Mice; D018396:Mucin-1 / Q000737:chemistry / Q000378:metabolism; D011108:Polymers / Q000737:chemistry*; D013745:Tetanus Toxoid / Q000737:chemistry / Q000378:metabolism
chemical_list: D000906:Antibodies; D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D018984:Epitopes, T-Lymphocyte; D006020:Glycopeptides; D018396:Mucin-1; D011108:Polymers; D013745:Tetanus Toxoid; C043941:polyglycerol; D005990:Glycerol
keywords: antitumor agents; glycopeptides; hyperbranched polymers; polyglycerol; tumor-associated MUC1