title: An ATPase inhibitory peptide with antibacterial and ion current effects.
abstract: An 84-residue bactericidal peptide, PSK, was purified from a Chrysomya megacephala fly larvae preparation. Its amino acid sequence is similar to that of a previously reported larval peptide of the Drosophila genus (SK84) noticed for its anticancer and antimicrobial properties. The PSK sequence is also homologous to mitochondrial ATPase inhibitors from insects to humans (35-65% sequence identity), indicating an intracellular protein target and possible mechanism for PSK. It contains a cluster of six glycine residues, and has several two- and three-residue repeats. It is active against both Gram-positive and Gram-negative bacteria via a mechanism apparently involving cell membrane disintegration and inhibition of ATP hydrolysis. In addition, PSK induces an inward cationic current in pancreatic β cells. Together, the findings identify a bioactive peptide of the ATPase inhibitor family with specific effects on both prokaryotic and mammalian cells.
authors.affiliation: Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, 1037 Luoyu Avenue, 430074 Wuhan, Hubei, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Lu
authors.affiliation: Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, 1037 Luoyu Avenue, 430074 Wuhan, Hubei, PR China.
authors.forename: Zheng-wang
authors.initials: ZW
authors.lastname: Chen
authors.affiliation: Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, 1037 Luoyu Avenue, 430074 Wuhan, Hubei, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, 1037 Luoyu Avenue, 430074 Wuhan, Hubei, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, 1037 Luoyu Avenue, 430074 Wuhan, Hubei, PR China.
authors.forename: Jiu-Ping
authors.initials: JP
authors.lastname: Ding
authors.affiliation: Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77 Stockholm, Sweden.
authors.forename: Ella
authors.initials: E
authors.lastname: Cederlund
authors.affiliation: Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77 Stockholm, Sweden.
authors.forename: Hans
authors.initials: H
authors.lastname: Jörnvall
authors.affiliation: Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77 Stockholm, Sweden. Electronic address: Tomas.Bergman@ki.se.
authors.forename: Tomas
authors.initials: T
authors.lastname: Bergman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D018407:Bacterial Physiological Phenomena / Q000187:drug effects*; D002460:Cell Line; D004175:Diptera / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D050417:Insulin-Secreting Cells / Q000187:drug effects / Q000502:physiology*; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology*; D007814:Larva / Q000378:metabolism; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry / Q000494:pharmacology*; D054328:Proton Pump Inhibitors / Q000737:chemistry / Q000494:pharmacology; D013329:Structure-Activity Relationship; D000097795:ATPase Inhibitory Protein
chemical_list: D000900:Anti-Bacterial Agents; D011506:Proteins; D054328:Proton Pump Inhibitors; D000097795:ATPase Inhibitory Protein
keywords: ATPase inhibitory peptide; Cellular cation current; Chrysomya megacephala peptide sequence; Glycine-rich antibacterial peptide; Pancreatic β cell