title: Influence of importin alpha/beta and exportin 1 on equine herpesvirus type 1 (EHV-1) replication in primary murine neurons.
abstract: Viruses replicating in the nucleus need to cross the nuclear membrane barrier during infection, therefore disruption of specific nuclear transport pathways is crucial for their replication cycle. In the present study we have investigated the influence of nucleo-cytoplasmic transport inhibitors -- ivermectin and leptomycin B, on EHV-1 replication in primary murine neurons. Obtained results suggest that the examined proteins -- exportin 1 and importin alpha/beta may participate, but are not required, during EHV-1 infection. Based on these results, it can be assumed that EHV-1 is able to use other receptors for nucleo-cytoplasmic transport.
authors.affiliation: Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences- SGGW, Ciszewskiego 8, 02-786 Warsaw, Poland. anex007@op.pl
authors.forename: A
authors.initials: A
authors.lastname: Slońska
authors.affiliation: Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences- SGGW, Ciszewskiego 8, 02-786 Warsaw, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Cymerys
authors.affiliation: Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences- SGGW, Nowoursynowska 159, 02-776, Warsaw, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Skwarska
authors.affiliation: Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences- SGGW, Ciszewskiego 8, 02-786 Warsaw, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Golke
authors.affiliation: Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences- SGGW, Ciszewskiego 8, 02-786 Warsaw, Poland.
authors.forename: M W
authors.initials: MW
authors.lastname: Bańbura
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology; D002478:Cells, Cultured; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D004861:Herpesvirus 1, Equid / Q000502:physiology*; D007306:Insecticides / Q000494:pharmacology; D007559:Ivermectin / Q000494:pharmacology; D028884:Karyopherins / Q000378:metabolism*; D051379:Mice; D009474:Neurons / Q000187:drug effects / Q000378:metabolism* / Q000821:virology*; D014779:Virus Replication / Q000502:physiology*
chemical_list: D000935:Antifungal Agents; D005231:Fatty Acids, Unsaturated; D007306:Insecticides; D028884:Karyopherins; D007559:Ivermectin; C038753:leptomycin B