title: Profiling of immunoglobulins in resting and mechanically stimulated saliva.
abstract: BACKGROUND
  Dysregulation of salivary immunoglobulins has been implicated in illnesses ranging from periodontal disease to HIV aids and malignant cancers. Despite these advances there is a lack of agreement among studies with regard to the salivary immunoglobulin levels in healthy controls.
  
  
  METHODS
  Resting and mechanically stimulated saliva samples and matching serum samples were collected from healthy individuals (n = 33; 40-55 years of age; gender: 23 female, 10 male). A matrix-matched AlphaLISA(®) assay was developed to determine the concentrations of IgG1 and IgG4 in serum and saliva samples.
  
  
  CONCLUSIONS
  Clear relationships were observed in the flow rate and concentration of each immunoglobulin in the two types of saliva. This study affirms the need to establish and standardize collection methods before salivary IgGs are used for diagnostic purposes.
authors.affiliation: Australian Institute of Bioengineering & Nanotechnology, University of Queensland, Queensland, Australia.
authors.forename: Michael
authors.initials: M
authors.lastname: Macdonald
authors.forename: Nur Aziemah Ab
authors.initials: NA
authors.lastname: Ghani
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Wan
authors.forename: Justin
authors.initials: J
authors.lastname: Cooper-White
authors.forename: Goce
authors.initials: G
authors.lastname: Dimeski
authors.forename: Chamindie
authors.initials: C
authors.lastname: Punyadeera
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods / Q000592:standards; D007074:Immunoglobulin G / Q000032:analysis* / Q000097:blood*; D008297:Male; D008875:Middle Aged; D012463:Saliva / Q000737:chemistry* / Q000276:immunology
chemical_list: D007074:Immunoglobulin G