title: Whole genome sequencing of the black grouse (Tetrao tetrix): reference guided assembly suggests faster-Z and MHC evolution.
abstract: BACKGROUND
  The different regions of a genome do not evolve at the same rate. For example, comparative genomic studies have suggested that the sex chromosomes and the regions harbouring the immune defence genes in the Major Histocompatability Complex (MHC) may evolve faster than other genomic regions. The advent of the next generation sequencing technologies has made it possible to study which genomic regions are evolutionary liable to change and which are static, as well as enabling an increasing number of genome studies of non-model species. However, de novo sequencing of the whole genome of an organism remains non-trivial. In this study, we present the draft genome of the black grouse, which was developed using a reference-guided assembly strategy.
  
  
  RESULTS
  We generated 133 Gbp of sequence data from one black grouse individual by the SOLiD platform and used a combination of de novo assembly and chicken reference genome mapping to assemble the reads into 4572 scaffolds with a total length of 1022 Mb. The draft genome well covers the main chicken chromosomes 1 ~ 28 and Z which have a total length of 1001 Mb. The draft genome is fragmented, but has a good coverage of the homologous chicken genes. Especially, 33.0% of the coding regions of the homologous genes have more than 90% proportion of their sequences covered. In addition, we identified ~1 M SNPs from the genome and identified 106 genomic regions which had a high nucleotide divergence between black grouse and chicken or between black grouse and turkey.
  
  
  CONCLUSIONS
  Our results support the hypothesis that the chromosome X (Z) evolves faster than the autosomes and our data are consistent with the MHC regions being more liable to change than the genome average. Our study demonstrates how a moderate sequencing effort can be combined with existing genome references to generate a draft genome for a non-model species.
authors.affiliation: Department of Ecology and Genetics, Evolutionary Biology Centre, Uppsala University, Norbyvägen 18D, SE-75236 Uppsala, Sweden. biao.wang@ebc.uu.se.
authors.forename: Biao
authors.initials: B
authors.lastname: Wang
authors.forename: Robert
authors.initials: R
authors.lastname: Ekblom
authors.forename: Ignas
authors.initials: I
authors.lastname: Bunikis
authors.forename: Heli
authors.initials: H
authors.lastname: Siitari
authors.forename: Jacob
authors.initials: J
authors.lastname: Höglund
mesh_terms: D000818:Animals; D001717:Birds / Q000235:genetics*; D002875:Chromosomes; D019295:Computational Biology / Q000379:methods; D019143:Evolution, Molecular; D016678:Genome; D023281:Genomics; D059014:High-Throughput Nucleotide Sequencing; D008285:Major Histocompatibility Complex / Q000235:genetics; D008297:Male; D058977:Molecular Sequence Annotation; D020641:Polymorphism, Single Nucleotide; D012091:Repetitive Sequences, Nucleic Acid