title: Evaluating the effect of low-level laser therapy on healing of tentomized Achilles tendon in streptozotocin-induced diabetic rats by light microscopical and gene expression examinations.
abstract: Tendon healing is impaired in individuals diagnosed with diabetes mellitus (DM). According to research, there is considerable improvement in the healing of surgically tenotomized Achilles tendons following low-level laser therapy (LLLT) in non-diabetic, healthy animals. This study uses light microscopic (LM) and semi-quantitative reverse transcription PCR (RT-PCR) analyses to evaluate the ability of LLLT in healing Achilles tendons from streptozotocin-induced diabetic (STZ-D) rats. A total of 88 rats were randomly divided into two groups, non-diabetic and diabetic. DM was induced in the rats by injections of STZ. The right Achilles tendons of all rats were tenotomized 1 month after administration of STZ. Laser-treated rats were treated with a helium-neon (He-Ne) laser that had a 632.8-nm wavelength and 7.2-mW average power. Experimental group rats received a daily dose of 0.014 J (energy density, 2.9 J/cm(2)). Control rats did not receive LLLT. Animals were sacrificed on days 5, 10, and 15 post-operatively for semi-quantitative LM and semi-quantitative RT-PCR examinations of transforming growth factor-beta1 (TGF-β1) gene expression. The chi-square test showed that LLLT significantly reduced inflammation in non-diabetic rats compared with their non-diabetic controls (p = 0.02). LLLT significantly decreased inflammation in diabetic rats on days 5 (p = 0.03) and 10 (p = 0.02) compared to the corresponding control diabetic rats. According to the student's t test, LLLT significantly increased TGF-β1 gene expression in healthy (p = 0.000) and diabetic (p = 0.000) rats compared to their relevant controls. The He-Ne laser was effective in altering the inflammatory reaction and increasing TGF-β1 gene production.
authors.affiliation: Department of Anatomical Sciences and Biology, Medical Faculty, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Morteza
authors.initials: M
authors.lastname: Aliodoust
authors.forename: Mohammad
authors.initials: M
authors.lastname: Bayat
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Jalili
authors.forename: Zainalabedin
authors.initials: Z
authors.lastname: Sharifian
authors.forename: Masoomeh
authors.initials: M
authors.lastname: Dadpay
authors.forename: Mohammad
authors.initials: M
authors.lastname: Akbari
authors.forename: Mehrnoush
authors.initials: M
authors.lastname: Bayat
authors.forename: Amir
authors.initials: A
authors.lastname: Khoshvaghti
authors.forename: Homa
authors.initials: H
authors.lastname: Bayat
mesh_terms: D000125:Achilles Tendon / Q000473:pathology / Q000503:physiopathology / Q000528:radiation effects; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000503:physiopathology; D015870:Gene Expression; D054020:Lasers, Gas / Q000627:therapeutic use*; D028022:Low-Level Light Therapy; D008297:Male; D051381:Rats; D013311:Streptozocin; D059467:Transcriptome; D014945:Wound Healing / Q000528:radiation effects*
chemical_list: D013311:Streptozocin