title: Nardosinone improves the proliferation, migration and selective differentiation of mouse embryonic neural stem cells.
abstract: In this study, we investigated the impact of Nardosinone, a bioactive component in Nardostachys root, on the proliferation and differentiation of neural stem cells. The neural stem cells were isolated from cerebrums of embryonic day 14 CD1 mice. The proliferation of cells was monitored using the cell counting kit-8 assay, bromodeoxyuridine incorporation and cell cycle analysis. Cell migration and differentiation were investigated with the neurosphere assay and cell specific markers, respectively. The results showed that Nardosinone promotes cells proliferation and increases cells migration distance in a dose-dependent manner. Nardosinone also induces the selective differentiation of neural stem cells to neurons and oligodendrocytes, as indicated by the expression of microtubule-associated protein-2 and myelin basic protein, respectively. Nardosinone also increases the expression of phospho-extracellular signal-regulated kinase and phospho-cAMP response element binding protein during proliferation and differentiation. In conclusion, this study reveals the regulatory effects of Nardosinone on neural stem cells, which may have significant implications for the treatment of brain injury and neurodegenerative diseases.
authors.affiliation: Department of Biomedical Science, College of Medicine, University of Toledo, Toledo, Ohio, United States of America; Department of Pharmacology, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Ze-Hui
authors.initials: ZH
authors.lastname: Li
authors.affiliation: Department of Pharmacology, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Pharmacology, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Jin-Li
authors.initials: JL
authors.lastname: Shi
authors.affiliation: Department of Pharmacology, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Min-Ke
authors.initials: MK
authors.lastname: Tang
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D051379:Mice; D039902:Multipotent Stem Cells / Q000166:cytology; D058953:Neural Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D009836:Oligodendroglia / Q000166:cytology / Q000187:drug effects; D000081223:Polycyclic Sesquiterpenes; D015203:Reproducibility of Results; D012717:Sesquiterpenes / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D018874:Spheroids, Cellular / Q000166:cytology / Q000187:drug effects
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; C011513:nardosinone; D048049:Extracellular Signal-Regulated MAP Kinases