title: A deep tissue fluorescence imaging system with enhanced SHG detection capabilities.
abstract: We describe a novel two-photon fluorescence microscopy system capable of producing high-quality second harmonic generation (SHG) images in thick turbid media by using an innovative detection system. This novel detection system is capable of detecting photons from a very large surface area. This system has proven effective in providing images of thick turbid samples, both biological and artificial. Due to its transmission detection geometry, the system is particularly suitable for detecting SHG signals, which are generally forward directed. In this article, we present comparative data acquired simultaneously on the same sample with the forward and epidetection schemes.
authors.affiliation: Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California Irvine, Irvine, California, 92697.
authors.forename: Viera
authors.initials: V
authors.lastname: Crosignani
authors.forename: Sohail
authors.initials: S
authors.lastname: Jahid
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Dvornikov
authors.forename: Enrico
authors.initials: E
authors.lastname: Gratton
mesh_terms: D000818:Animals; D024042:Collagen Type I; D008810:Mice, Inbred C57BL; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D018485:Muscle Fibers, Skeletal / Q000648:ultrastructure; D061848:Optical Imaging / Q000379:methods*; D055620:Optical Phenomena; D017785:Photons; D012828:Silicones
chemical_list: D024042:Collagen Type I; D012828:Silicones
keywords: deep tissue imaging; second harmonic generation; two-photon microscopy