title: Biodistribution of adult derived human liver stem cells following intraportal infusion in a 17-year-old patient with glycogenosis type 1A.
abstract: BACKGROUND
  Current treatment of inherited liver inborn errors of metabolism in children consists in appropriate diet and drugs and, for unstable patients, final orthotopic liver transplantation. Unfortunately, liver transplantation remains not easily available because of organ shortage and imposes inherent risks and lifelong immunosuppressive therapy. Therefore alternative treatments are required. Hepatocytes transplantation and its limitations led to consider innovative alternative such as transplantation of adult derived human liver stem cells (ADLHSC). These cells present high proliferative capacity, good resistance to cryopreservation and ability to differentiate into hepatocyte-like cells displaying mature hepatocyte functions.
  
  
  OBJECTIVE
  Biodistribution of ADHLSC had never been assessed after infusion through the portal vein in patients. This information is required to determine the safety of the method.
  
  
  METHODS
  ADHLSC were efficiently labelled with 111-Indium DTPA radiotracer and SPECT imaging was used for the acquisition of whole body imaging to document short term biodistribution of ADHLSC.
  
  
  RESULTS
  Following infusion through the portal vein, ADHLSC diffused homogenously throughout the liver and remained strictly within the targeted organ. Images were acquired until 5 days after infusion. At that time, no signal was observed in any other organs except the liver. Urinary excretion of 111-Indium DTPA was also monitored.
  
  
  CONCLUSIONS
  For the first time, we documented the short term biodistribution of ADHLSC within the liver after infusion through the portal vein.
authors.affiliation: Université Catholique de Louvain, Institut de Recherche Expérimentale et Clinique, Paediatric Hepatology and Cell Therapy, Avenue Hippocrate 10, 1200 Brussels, Belgium. Electronic address: florence.defresne@uclouvain.be.
authors.forename: Florence
authors.initials: F
authors.lastname: Defresne
authors.affiliation: Université Catholique de Louvain, Institut de Recherche Expérimentale et Clinique, Paediatric Hepatology and Cell Therapy, Avenue Hippocrate 10, 1200 Brussels, Belgium.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Tondreau
authors.affiliation: Université Catholique de Louvain, Institut de Recherche Expérimentale et Clinique, Paediatric Hepatology and Cell Therapy, Avenue Hippocrate 10, 1200 Brussels, Belgium.
authors.forename: Xavier
authors.initials: X
authors.lastname: Stéphenne
authors.affiliation: Université Catholique de Louvain, Institut de Recherche Expérimentale et Clinique, Paediatric Hepatology and Cell Therapy, Avenue Hippocrate 10, 1200 Brussels, Belgium.
authors.forename: Françoise
authors.initials: F
authors.lastname: Smets
authors.affiliation: Université Catholique de Louvain, Institut de Recherche Expérimentale et Clinique, Paediatric Hepatology and Cell Therapy, Avenue Hippocrate 10, 1200 Brussels, Belgium.
authors.forename: Annick
authors.initials: A
authors.lastname: Bourgois
authors.affiliation: Université Catholique de Louvain, Institut de Recherche Expérimentale et Clinique, Paediatric Hepatology and Cell Therapy, Avenue Hippocrate 10, 1200 Brussels, Belgium.
authors.forename: Mustapha
authors.initials: M
authors.lastname: Najimi
authors.affiliation: Cliniques universitaires Saint-Luc, Department of Radiology, Avenue Hippocrate 10, 1200 Brussels, Belgium.
authors.forename: François
authors.initials: F
authors.lastname: Jamar
authors.affiliation: Université Catholique de Louvain, Institut de Recherche Expérimentale et Clinique, Paediatric Hepatology and Cell Therapy, Avenue Hippocrate 10, 1200 Brussels, Belgium. Electronic address: etienne.sokal@uclouvain.be.
authors.forename: Etienne M
authors.initials: EM
authors.lastname: Sokal
mesh_terms: D000293:Adolescent; D053687:Adult Stem Cells / Q000378:metabolism* / Q000637:transplantation*; D005953:Glycogen Storage Disease Type I / Q000378:metabolism / Q000628:therapy* / Q000652:urine; D006801:Humans; D007205:Indium Radioisotopes / Q000652:urine; D008099:Liver / Q000166:cytology*; D014018:Tissue Distribution
chemical_list: D007205:Indium Radioisotopes
keywords: Biodistribution; Glycogenosis; Hepatic progenitor cells