title: A starch-binding domain identified in α-amylase (AmyP) represents a new family of carbohydrate-binding modules that contribute to enzymatic hydrolysis of soluble starch.
abstract: A novel starch-binding domain (SBD) that represents a new carbohydrate-binding module family (CBM69) was identified in the α-amylase (AmyP) of the recently established alpha-amylase subfamily GH13_37. The SBD and its homologues come mostly from marine bacteria, and phylogenetic analysis indicates that they are closely related to the CBM20 and CBM48 families. The SBD exhibited a binding preference toward raw rice starch, but the truncated mutant (AmyPΔSBD) still retained similar substrate preference. Kinetic analyses revealed that the SBD plays an important role in soluble starch hydrolysis because different catalytic efficiencies have been observed in AmyP and the AmyPΔSBD.
authors.affiliation: Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, School of Life Sciences, Anhui University, Hefei 230039, Anhui, PR China. Electronic address: pph0259@126.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Peng
authors.affiliation: Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, School of Life Sciences, Anhui University, Hefei 230039, Anhui, PR China.
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, School of Life Sciences, Anhui University, Hefei 230039, Anhui, PR China.
authors.forename: Maojiao
authors.initials: M
authors.lastname: Chen
authors.affiliation: Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, School of Life Sciences, Anhui University, Hefei 230039, Anhui, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, School of Life Sciences, Anhui University, Hefei 230039, Anhui, PR China.
authors.forename: Yazhong
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, School of Life Sciences, Anhui University, Hefei 230039, Anhui, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Gao
mesh_terms: D000327:Adsorption; D000595:Amino Acid Sequence; D059001:Aquatic Organisms / Q000201:enzymology; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D001665:Binding Sites; D006868:Hydrolysis; D007700:Kinetics; D008390:Markov Chains; D008969:Molecular Sequence Data; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D013213:Starch / Q000737:chemistry*; D013379:Substrate Specificity; D014733:Vibrio / Q000201:enzymology / Q000235:genetics; D000516:alpha-Amylases / Q000737:chemistry* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D013213:Starch; D000516:alpha-Amylases
keywords: Binding preference; Carbohydrate-binding module; Soluble starch hydrolysis; Starch-binding domain; α-Amylase