title: Diffraction phase microscopy: retrieving phase contours on living cells with a wavelet-based space-scale analysis.
abstract: We propose a two-dimensional (2-D) space-scale analysis of fringe patterns collected from a diffraction phase microscope based on the 2-D Morlet wavelet transform. We show that the adaptation of a ridge detection method with anisotropic 2-D Morlet mother wavelets is more efficient for analyzing cellular and high refractive index contrast objects than Fourier filtering methods since it can separate phase from intensity modulations. We compare the performance of this ridge detection method on theoretical and experimental images of polymer microbeads and experimental images collected from living myoblasts.
authors.affiliation: Université de Lyon, CNRS UMR5672, ENS de Lyon, 69007 France.
authors.forename: Cristina
authors.initials: C
authors.lastname: Martinez-Torres
authors.affiliation: Université de Lyon, CNRS USR3010, ENS de Lyon, 69007 France.
authors.forename: Lotfi
authors.initials: L
authors.lastname: Berguiga
authors.affiliation: Université de Lyon, CNRS UMR5672, ENS de Lyon, 69007 FrancecUniversité de Lyon, CNRS UMR5239, ENS de Lyon, 69007 France.
authors.forename: Laura
authors.initials: L
authors.lastname: Streppa
authors.affiliation: Université de Lyon, CNRS UMR5672, ENS de Lyon, 69007 France.
authors.forename: Elise
authors.initials: E
authors.lastname: Boyer-Provera
authors.affiliation: Université de Lyon, CNRS UMR5239, ENS de Lyon, 69007 France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Schaeffer
authors.affiliation: Université Bordeaux 1-ENITAB, CNRS UMR 5248, Pessac, 33600 France.
authors.forename: Juan
authors.initials: J
authors.lastname: Elezgaray
authors.affiliation: Université de Lyon, CNRS UMR5672, ENS de Lyon, 69007 France.
authors.forename: Alain
authors.initials: A
authors.lastname: Arneodo
authors.affiliation: Université de Lyon, CNRS UMR5672, ENS de Lyon, 69007 France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Argoul
mesh_terms: D000818:Animals; D002460:Cell Line; D003584:Cytological Techniques / Q000379:methods*; D051379:Mice; D008857:Microscopy, Interference / Q000379:methods*; D015203:Reproducibility of Results; D058067:Wavelet Analysis