title: Litopenaeus vannamei NF-κB is required for WSSV replication.
abstract: Many viruses can hijack the host cell NF-κB as part of their life cycle, diverting NF-κB immune regulatory functions to favor their replications. There were several reports on the functions of Litopenaeus vannamei NF-κB (LvNF-κB) in White spot syndrome virus (WSSV) replication in vitro. Here, we studied the relationship between LvNF-κB family protein Dorsal (LvDorsal) and Relish (LvRelish) with WSSV replication in vivo. The expressions of LvDorsal and LvRelish were significantly upregulated by WSSV challenge. Virus loads and expression of viral envelope protein VP28 in LvDorsal or LvRelish silencing shrimps were significantly lower than the control shrimps injected with EGFP-dsRNA or PBS after challenge with 1×10(5) copies WSSV/shrimp. In addition to the LvDorsal activation of WSV069 (ie1) and WSV303 promoter that we have reported, LvRelish can also activate WSV069 (ie1) and WSV303 promoter by dual luciferase reporter assays through screening 40 WSSV gene promoters that have putative multiple NF-κB binding sites. The promoter activity of the WSV069 (ie1) by LvDorsal activation was significantly higher than that by LvRelish activation. WSSV replication in LvDorsal, LvRelish or WSV303 silencing shrimps were significantly inhibited. These results indicate that the L. vannamei NF-κB family proteins LvDorsal and LvRelish expressions are significantly activated by WSSV challenge and WSSV replication partially relied on the activations of LvDorsal and LvRelish in vivo.
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, School of Marine Sciences, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qiu
authors.affiliation: School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhang
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, School of Marine Sciences, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: Yong-Gui
authors.initials: YG
authors.lastname: Chen
authors.affiliation: School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: Pei-Hui
authors.initials: PH
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: Xiao-Peng
authors.initials: XP
authors.lastname: Xu
authors.affiliation: School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: Chao-zheng
authors.initials: CZ
authors.lastname: Li
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, School of Marine Sciences, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: Yi-Hong
authors.initials: YH
authors.lastname: Chen
authors.affiliation: School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: Wen-Zhou
authors.initials: WZ
authors.lastname: Fan
authors.affiliation: School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yan
authors.affiliation: School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Weng
authors.affiliation: Fisheries College, Guangdong Ocean University, Zhanjiang, PR China.
authors.forename: Siuming
authors.initials: S
authors.lastname: FrancisChan
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, School of Marine Sciences, Sun Yat-sen University, Guangzhou, PR China; School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China. Electronic address: lsshjg@mail.sysu.edu.cn.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: He
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000502:physiology*; D005753:Gastric Mucosa / Q000821:virology; D015967:Gene Expression Regulation, Viral; D005880:Gills / Q000378:metabolism / Q000821:virology; D054884:Host-Pathogen Interactions; D016328:NF-kappa B / Q000502:physiology*; D033561:Penaeidae / Q000378:metabolism* / Q000821:virology; D011401:Promoter Regions, Genetic; D015854:Up-Regulation; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication; D046848:White spot syndrome virus 1 / Q000502:physiology*
chemical_list: D060829:Arthropod Proteins; D016328:NF-kappa B; C548473:VP28 protein, white spot syndrome virus; D014759:Viral Envelope Proteins
keywords: Litopenaeus vannamei; LvDorsal; LvRelish; WSSV