title: Fast monocyte-derived dendritic cell-based immunotherapy.
abstract: Recent reports have described a new strategy for differentiation and maturation of monocyte (Mo)-derived dendritic cells (DC) within only 48-72 h of in vitro culture (fast-DC). Mature fast-DC are as effective as mature standard-DC (generated in 7-10 days of in vitro culture) in priming and propagation of antigen-specific T-cell responses. The use of fast-DC not only reduces labor and supply cost, as well as workload and time, but also increases the DC yield from Mo, which may facilitate DC-based immunotherapy for cancer patients. Detailed protocols for generation, pulsing with different antigen sources, and transduction with adenoviral vector of Mo-derived mature fast-DC as well as using of fast-DC for priming and propagation of antigen-specific cytotoxic T-cell effectors will be described here.
authors.affiliation: Zoology Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
authors.forename: Gamal
authors.initials: G
authors.lastname: Ramadan
mesh_terms: D002469:Cell Separation; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology*; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods*; D009000:Monocytes / Q000166:cytology*; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology; D013997:Time Factors; D014161:Transduction, Genetic