title: [Substrate specificity of 3-hydroxypicolinic acid unit in the viridogrisein biosynthetic pathway].
abstract: OBJECTIVE
  To explore the role of sgvS in the biosynthesis of 3-hydroxypicolinic acid moiety of viridogrsein, and to analyze the substrate specificity of 3-hydroxypicolinic acid moiety in the viridogrsein biosynthetic pathway.
  
  
  METHODS
  Through gene insertion inactivation and in trans complementation strategies, we obtained the gene inactivation mutant sgvS andits complementation mutant deltasgvS :: sgvS. Meanwhile, we fed 3-hydroxypicolinic acid, picolinic acid, 2-piperidinecarboxylic acid, 3-chloropyridinecarboxylic acid, 4-chloropyridinecarboxylic acid, 3,5-chloropyridinecarboxylic acid, nicotinic acid, 2-chloronicotinic acid, 2-fluoronicotinic acid acid, 5-fluoronicotinic acid and 6-fluoronicotinic acid to the sgvS mutant, respectively. The fermentation extracts were analyzed by HPLC.
  
  
  RESULTS
  sgvS mutant abolished the viridogrsein production; viridogrsein production was restored through in trans complementation of sgvS mutant or by feeding 3-hydroxypicolinic acid to the sgvS mutant. No new viridogrsein analogues were observed by feeding other above mentioned 3-hydroxypicolinic acid analogues tothe deltasgvS mutant.
  
  
  CONCLUSIONS
  sgvS is necessary for the biosynthesis of 3-hydroxypicolinic acid moiety. The biosynthetic protein, SgvD1, activates 3-hydroxypicolinic acid, showing strict substrate specificity en route to the viridogrsein biosynthesis.
authors.forename: Yunchang
authors.initials: Y
authors.lastname: Xie
authors.forename: Jianhua
authors.initials: J
authors.lastname: Ju
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D053898:Biosynthetic Pathways; D005285:Fermentation; D018942:Macrolides / Q000378:metabolism*; D010848:Picolinic Acids / Q000378:metabolism*; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity; D047630:Depsipeptides
chemical_list: D000900:Anti-Bacterial Agents; D018942:Macrolides; D010848:Picolinic Acids; C049246:3-hydroxypicolinic acid; C004910:etamycin; D047630:Depsipeptides