title: Production of extracellular matrix proteins by human pulp fibroblasts in contact with papacárie and carisolv.
abstract: OBJECTIVE
  To evaluate the effect of two products for the chemomecanical removal of carious tissue (Papacárie and Carisolv) on human dental pulp fibroblasts and the synthesis of extracellular matrix proteins.
  
  
  METHODS
  Fibroblasts were divided into three groups: group 1 (control), group 2 (Papacárie) and group 3 (Carisolv). Collagen I, III , fibronectin and osteonectin were analysed by immunofluorescence and compared among the groups.
  
  
  RESULTS
  The groups exhibited similar immunolabeling for vimentin, type I collagen and fibronectin, but were negative for type III collagen. Osteonectin staining was strongly positive in the cells treated with Papacárie and Carisolv and weakly positive in the control group.
  
  
  CONCLUSIONS
  The findings of the present study showed that Papacárie and Carisolv are not cytotoxic to pulp fibroblast cells. Moreover, these products stimulate fibroblasts to produce osteonectin, likely leading to the formation of dentin matrix. These findings confirm the safe, beneficial use of both gels in minimally invasive techniques.
authors.forename: Sandra Kalil
authors.initials: SK
authors.lastname: Bussadori
authors.forename: Olga Maria
authors.initials: OM
authors.lastname: Amancio
authors.forename: Manoela Domingues
authors.initials: MD
authors.lastname: Martins
authors.forename: Carolina Cardoso
authors.initials: CC
authors.lastname: Guedes
authors.forename: Thays Almeida
authors.initials: TA
authors.lastname: Alfaya
authors.forename: Elaine Marcílio
authors.initials: EM
authors.lastname: Santos
authors.forename: Cristiane Miranda
authors.initials: CM
authors.lastname: França