title: Preparation and adsorption of bovine serum albumin-imprinted polyacrylamide hydrogel membrane grafted on non-woven polypropylene.
abstract: Bovine serum albumin (BSA) imprinted polypropylene (PP) fiber-grafted polyacrylamide (PAM) hydrogel membrane (PP-g-PAM MIP) was prepared using non-woven PP fiber as matrix, BSA as template molecule, and acrylamide (AM) as functional monomer via UV radiation-reduced polymerization in an aqueous phase. SEM, FT-IR, DSC and TG were used to characterize the PP grafted PAM hydrogel. Influence factors on the adsorption capacity of PP-g-PAM MIP were investigated, such as monomer concentration, cross-linker concentration, template molecule amount and pH values in BSA solution. The adsorption and recognition properties of PP-g-PAM MIP were evaluated and the results showed that the PP-g-PAM MIP exhibited an obvious improvement in terms of adsorption capacity for BSA as compared with non-imprinted ones. PP-g-PAM MIPs could recognize the template protein using Lys, Ova, BHb, and Glo as control proteins, and the selectivity factor (β) was above 2.0. The imprinting efficiency of PP-g-PAM MIP tended to be stable after three cycles and maintained 76% of the initial value of the imprinting efficiency even after five repetitions, which was more excellent than that of PAM microsphere. The PP-g-PAM MIP is low cost and easy to be prepared, which would show its potential applications in the fields of extracting and testing required proteins from cells or particulate samples.
authors.affiliation: State Key Laboratory of Hollow Fiber Membrane Materials and Processes, Tianjin Polytechnic University, Tianjin 300387, China; School of Material Science and Engineering, Tianjin Polytechnic University, Tianjin 300387, China. Electronic address: zhaokongyin@tjpu.edu.cn.
authors.forename: Kongyin
authors.initials: K
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Hollow Fiber Membrane Materials and Processes, Tianjin Polytechnic University, Tianjin 300387, China; School of Material Science and Engineering, Tianjin Polytechnic University, Tianjin 300387, China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Hollow Fiber Membrane Materials and Processes, Tianjin Polytechnic University, Tianjin 300387, China; School of Material Science and Engineering, Tianjin Polytechnic University, Tianjin 300387, China.
authors.forename: Wenkui
authors.initials: W
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Hollow Fiber Membrane Materials and Processes, Tianjin Polytechnic University, Tianjin 300387, China; School of Material Science and Engineering, Tianjin Polytechnic University, Tianjin 300387, China.
authors.forename: Lingzhi
authors.initials: L
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Hollow Fiber Membrane Materials and Processes, Tianjin Polytechnic University, Tianjin 300387, China; School of Material Science and Engineering, Tianjin Polytechnic University, Tianjin 300387, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Hollow Fiber Membrane Materials and Processes, Tianjin Polytechnic University, Tianjin 300387, China. Electronic address: jfwei@tjpu.edu.cn.
authors.forename: Junfu
authors.initials: J
authors.lastname: Wei
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D002152:Calorimetry, Differential Scanning; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D011126:Polypropylenes / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry
chemical_list: D000180:Acrylic Resins; D008567:Membranes, Artificial; D011126:Polypropylenes; C016680:polyacrylamide gels; D012710:Serum Albumin, Bovine
keywords: Adsorption; Grafting; Membrane; Polyacrylamide hydrogel; Polypropylene non-woven; Protein molecular imprinting