title: Lipid composition of thermophilic Geobacillus sp. strain GWE1, isolated from sterilization oven.
abstract: GWE1 strain is an example of anthropogenic thermophilic bacterium, recently isolated from dark crusty material from sterilization ovens by Correa-Llantén et al. (Kor. J. Microb. Biotechnol. 2013. 41(3):278-283). Thermostability is likely to arise from the adaptation of macromolecules such as proteins, lipids and nucleic acids. Complex lipid arrangement and/or type in the cell membrane are known to affect thermostability of microorganisms and efforts were made to understand the chemical nature of the polar lipids of membrane. In this work, we extracted total lipids from GWE1 cell membrane, separated them by TLC into various fractions and characterize the lipid structures of certain fractions with analytical tools such as (1)H, (13)C, (31)P and 2D NMR spectroscopy, ATR-FTIR spectroscopy and MS(n) spectrometry. We were able to identify glycerophosphoethanolamine, glycerophosphate, glycerophosphocholine, glycerophosphoglycerol and cardiolipin lipid classes and an unknown glycerophospholipid class with novel MS/MS spectra pattern. We have also noticed the presence of saturated iso-branched fatty acids with NMR spectra in individual lipid classes.
authors.affiliation: Department of Chemistry, Temple University, 1901 N 13th Street, Philadelphia, PA, USA.
authors.forename: Siddharth P
authors.initials: SP
authors.lastname: Shah
authors.affiliation: Department of Chemistry, Temple University, 1901 N 13th Street, Philadelphia, PA, USA. Electronic address: suebee@temple.edu.
authors.forename: Susan A
authors.initials: SA
authors.lastname: Jansen
authors.affiliation: Department of Biochemistry, Temple University School of Medicine, Philadelphia, PA, USA.
authors.forename: Leeandrew Jacques-Asa
authors.initials: LJ
authors.lastname: Taylor
authors.affiliation: Department of Biochemistry, Temple University School of Medicine, Philadelphia, PA, USA.
authors.forename: Parkson Lee-Gau
authors.initials: PL
authors.lastname: Chong
authors.affiliation: Fundación Científica y Cultural Biociencia, José Domingo Cañas 2280, Ñuñoa, Santiago, Chile.
authors.forename: Daniela N
authors.initials: DN
authors.lastname: Correa-Llantén
authors.affiliation: Fundación Científica y Cultural Biociencia, José Domingo Cañas 2280, Ñuñoa, Santiago, Chile; Universidad de Santiago de Chile, Av Libertador Bernardo O'Higgins 3363, Santiago, Chile.
authors.forename: Jenny M
authors.initials: JM
authors.lastname: Blamey
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D003890:Desiccation; D056506:Geobacillus / Q000737:chemistry* / Q000166:cytology / Q000302:isolation & purification* / Q000528:radiation effects; D005990:Glycerol / Q000737:chemistry; D006358:Hot Temperature; D008055:Lipids / Q000032:analysis* / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry; D013242:Sterilization
chemical_list: D008055:Lipids; D010743:Phospholipids; D005990:Glycerol
keywords: Ester lipid; NMR spectroscopy; Shotgun lipidomics; Thermophiles