title: A journey through PROTEOSONICS.
abstract: Ultrasonic energy is gaining momentum in Proteomics. It helps to shorten many proteomics workflows in an easy and efficient manner. Ultrasonic energy is nowadays used for protein extraction, solubilisation and cell disruption, to speed protein identification, protein quantification, peptide profiling, metal-protein complexes characterisation and imaging mass spectrometry. The present review gives a perspective of the latest achievements in ultrasonic-based sample treatment for proteomics as well as provides the basic concepts and the tools of the trade to efficiently implement this tool in proteomics labs.
authors.affiliation: BIOSCOPE Research group, REQUIMTE, Department of Chemistry, Faculty of Science and Technology, University NOVA of Lisbon, Portugal; PROTEOMASS Scientific Society, Madan Parque, Rua dos Inventores, 2825-182 Caparica, Portugal.
authors.forename: J E
authors.initials: JE
authors.lastname: Araújo
authors.affiliation: BIOSCOPE Research group, REQUIMTE, Department of Chemistry, Faculty of Science and Technology, University NOVA of Lisbon, Portugal; PROTEOMASS Scientific Society, Madan Parque, Rua dos Inventores, 2825-182 Caparica, Portugal.
authors.forename: E
authors.initials: E
authors.lastname: Oliveira
authors.affiliation: Turku Centre for Biotechnology, University of Turku & Abo Akademi University, Turku, Finland.
authors.forename: P
authors.initials: P
authors.lastname: Kouvonen
authors.affiliation: Turku Centre for Biotechnology, University of Turku & Abo Akademi University, Turku, Finland.
authors.forename: G L
authors.initials: GL
authors.lastname: Corthals
authors.affiliation: BIOSCOPE Research group, REQUIMTE, Department of Chemistry, Faculty of Science and Technology, University NOVA of Lisbon, Portugal; PROTEOMASS Scientific Society, Madan Parque, Rua dos Inventores, 2825-182 Caparica, Portugal.
authors.forename: C
authors.initials: C
authors.lastname: Lodeiro
authors.affiliation: BIOSCOPE Research group, REQUIMTE, Department of Chemistry, Faculty of Science and Technology, University NOVA of Lisbon, Portugal; PROTEOMASS Scientific Society, Madan Parque, Rua dos Inventores, 2825-182 Caparica, Portugal. Electronic address: hmsantos@fct.unl.pt.
authors.forename: H M
authors.initials: HM
authors.lastname: Santos
authors.affiliation: BIOSCOPE Research group, REQUIMTE, Department of Chemistry, Faculty of Science and Technology, University NOVA of Lisbon, Portugal; PROTEOMASS Scientific Society, Madan Parque, Rua dos Inventores, 2825-182 Caparica, Portugal. Electronic address: jlcm@fct.unl.pt.
authors.forename: J L
authors.initials: JL
authors.lastname: Capelo
mesh_terms: D040901:Proteomics; D014465:Ultrasonics
keywords: Protein; Proteomics; Review; Sample preparation; Ultrasonic; Ultrasound