title: Multigene phylogenies of Ophiostomataceae associated with Monterey pine bark beetles in Spain reveal three new fungal species.
abstract: Ophiostoma species, some of which cause sapstain in timber and/or are mild pathogens, are common fungal associates of bark beetles (Coleoptera: Scolytinae). Three new Ophiostomataceae from Spain are recognized in the present study based on comparisons of sequence data for three gene regions as well as morphological characteristics. The new taxa are described as Ophiostoma nebulare sp. nov., Ophiostoma euskadiense sp. nov. and Graphilbum crescericum sp. nov.
authors.affiliation: Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria 0002, South Africa.
authors.forename: Pedro
authors.initials: P
authors.lastname: Romón
authors.forename: Z Wilhelm
authors.initials: ZW
authors.lastname: de Beer
authors.forename: XuDong
authors.initials: X
authors.lastname: Zhou
authors.forename: Tuan A
authors.initials: TA
authors.lastname: Duong
authors.forename: Brenda D
authors.initials: BD
authors.lastname: Wingfield
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Wingfield
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000382:microbiology*; D008969:Molecular Sequence Data; D055155:Ophiostomatales / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D010802:Phylogeny; D028223:Pinus / Q000469:parasitology*; D013030:Spain; D013172:Spores, Fungal / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification
keywords: calmodulin gene; morphology; rRNA internal transcribed spacers; sequencing; β-tubulin gene