title: Nanoparticles of star-like copolymer mannitol-functionalized poly(lactide)-vitamin E TPGS for delivery of paclitaxel to prostate cancer cells.
abstract: The purpose of this research was to develop novel nanoparticles (NPs) of star-like copolymer mannitol-functionalized poly(lactide)-vitamin E TPGS (M-PLA-TPGS) for paclitaxel delivery for prostate cancer treatment, and evaluate their therapeutic effects in prostate cancer cell line and animal model in comparison with the linear PLGA NPs and poly(lactide)-vitamin E TPGS (PLA-TPGS) NPs. The paclitaxel-loaded M-PLA-TPGS NPs, prepared by a modified nano-precipitation method, were observed by FESEM to be near-spherical shape with narrow size distribution. The drug-loaded NPs were further characterized in terms of size, surface charge, drug content, encapsulation efficiency and in vitro drug release. The results showed that the M-PLA-TPGS NPs were found to be stable, showing almost no change in particle size and surface charge during the three-month storage period. In vitro drug release exhibited biphasic pattern with initial burst release followed by slow and continuous release. The cellular uptake level of M-PLA-TPGS NPs was demonstrated higher than linear PLGA NPs and PLA-TPGS NPs in PC-3 cells. The data also showed that the paclitaxel-loaded M-PLA-TPGS nanoparticles have higher antitumor efficacy than that of linear PLA-TPGS nanoparticles and PLGA nanoparticles in vitro and in vivo. In summary, the star-like copolymer M-PLA-TPGS could be used as a potential and promising molecular biomaterial in developing novel nanoformulation for prostate cancer treatment.
authors.affiliation: Department of Urology, Nanshan Affiliated Hospital of Guangdong Medical College, Shenzhen, Guangdong, China.
authors.forename: Kebing
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, People's Hospital of New District Longhua, Shenzhen, Guangdong Shenzhen, China.
authors.forename: Longhua
authors.initials: L
authors.lastname: Guo
authors.affiliation: Department of Urology, Nanshan Affiliated Hospital of Guangdong Medical College, Shenzhen, Guangdong, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xiong
authors.affiliation: The Shenzhen Key Lab of Gene and Antibody Therapy, Center for Biotech and BioMedicine, Division of Life and Health Sciences, Graduate School at Shenzhen, Tsinghua University, Shenzhen, Guangdong, China Northeastern University, Boston, MA, USA alice_sunll@163.com zhengy@sz.tsinghua.edu.cn.
authors.forename: Leilei
authors.initials: L
authors.lastname: Sun
authors.affiliation: The Shenzhen Key Lab of Gene and Antibody Therapy, Center for Biotech and BioMedicine, Division of Life and Health Sciences, Graduate School at Shenzhen, Tsinghua University, Shenzhen, Guangdong, China School of Life Sciences, Tsinghua University, Beijing, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage* / Q000627:therapeutic use; D045744:Cell Line, Tumor; D006801:Humans; D008297:Male; D053758:Nanoparticles; D017239:Paclitaxel / Q000008:administration & dosage* / Q000627:therapeutic use; D011091:Polyesters / Q000008:administration & dosage*; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000473:pathology; D066244:Proton Magnetic Resonance Spectroscopy; D014810:Vitamin E / Q000008:administration & dosage*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D011091:Polyesters; D014810:Vitamin E; C033616:poly(lactide); D017239:Paclitaxel
keywords: Star-like copolymer; drug delivery; nanoparticles; paclitaxel; prostate cancer