title: [Incidence and distribution of the genus Armillaria in vineyards of the five protected designation of origin wines in Galicia (Northwestern Spain)].
abstract: BACKGROUND
  The genus Armillaria, specifically Armillaria mellea, is an important phytopathological problem in the wine sector in Galicia (NW Spain), having caused yield reductions in vineyards for the last 15 years. The fungus attacks the root system, resulting in a decrease in vigour, and eventually in the death of the plant. Up to now, there is no chemical or biological method really effective against the pathogen once it has infected the plant.
  
  
  OBJECTIVE
  The main objective of this work was to study the incidence and distribution of the genus Armillaria across the five Galician protected designation of origin (DO) wines (namely Rías Baixas, Ribeiro, Ribeira Sacra, Valdeorras and Monterrei) through the application of molecular techniques.
  
  
  METHODS
  A total of 624 samples (483 soils and 141 symptomatic vines) were analyzed by nested-PCR/RFLP, PCR-RFLP and phylogeny.
  
  
  RESULTS
  Armillaria mellea is widely distributed in vineyards of the five DO wines, with the highest incidence in the Ribeiro DO.
  
  
  CONCLUSIONS
  Preventive control measures against Armillaria mellea must be established in the five DO wines of Galicia, in order to reduce the advance of white root rot.
authors.affiliation: Estación Fitopatolóxica do Areeiro, Deputación de Pontevedra, Pontevedra, España. Electronic address: olga.aguin@depo.es.
authors.forename: Olga
authors.initials: O
authors.lastname: Aguín
authors.affiliation: Centro Tecnológico Agroalimentario de Lugo (CETAL), Lugo, España.
authors.forename: María
authors.initials: M
authors.lastname: Abuín
authors.affiliation: Centro Tecnológico Agroalimentario de Lugo (CETAL), Lugo, España.
authors.forename: Francisco
authors.initials: F
authors.lastname: Lozano
authors.affiliation: Estación Fitopatolóxica do Areeiro, Deputación de Pontevedra, Pontevedra, España.
authors.forename: Vanesa
authors.initials: V
authors.lastname: Ferreiroa
authors.affiliation: Centro Tecnológico Agroalimentario de Lugo (CETAL), Lugo, España.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Corral
authors.affiliation: Estación Fitopatolóxica do Areeiro, Deputación de Pontevedra, Pontevedra, España.
authors.forename: J Pedro
authors.initials: JP
authors.lastname: Mansilla
mesh_terms: D055392:Armillaria / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D016533:Mycological Typing Techniques; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D018517:Plant Roots / Q000382:microbiology; D013030:Spain; D013045:Species Specificity; D027843:Vitis / Q000382:microbiology*; D014920:Wine
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer
keywords: Armillaria mellea; Molecular techniques; Podredumbre blanca radicular; Técnicas moleculares; Vitis vinifera; White root rot