title: Plaque reduction neutralization antibody test does not accurately predict protection against dengue infection in Ratchaburi cohort, Thailand.
abstract: BACKGROUND
  The plaque reduction neutralization test (PRNT) is currently the best and most widely accepted approach to measuring virus-neutralizing and protective antibodies to dengue virus, and in assessing the immunogenicity of a dengue vaccine. However, the correlation between presence of dengue-neutralizing antibody and protection from infection is not absolute.
  
  
  RESULTS
  In a cohort study in Ratchaburi Province, Thailand, 48 subjects with serologically confirmed symptomatic dengue infection were tested for pre-existing dengue neutralizing antibody using PRNT. Nine subjects had quite high pre-existing PRNT50 titers (titer >90) to subsequent infecting dengue serotypes, but still had symptomatic infections.
  
  
  CONCLUSIONS
  This report provides evidence that PRNT may not be a good test for predicting protection against subsequent dengue infection.
authors.affiliation: Department of Tropical Pediatrics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. chukiat.sir@mahidol.ac.th.
authors.forename: Chukiat
authors.initials: C
authors.lastname: Sirivichayakul
authors.forename: Arunee
authors.initials: A
authors.lastname: Sabchareon
authors.forename: Kriengsak
authors.initials: K
authors.lastname: Limkittikul
authors.forename: Sutee
authors.initials: S
authors.lastname: Yoksan
mesh_terms: D000293:Adolescent; D057134:Antibodies, Neutralizing / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D002648:Child; D015331:Cohort Studies; D003715:Dengue / Q000276:immunology*; D003716:Dengue Virus / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D009500:Neutralization Tests / Q000379:methods*; D013785:Thailand; D010948:Viral Plaque Assay / Q000379:methods*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral