title: Antinociceptive effects of sustained-release buprenorphine in a model of incisional pain in rats (Rattus norvegicus).
abstract: Effective management of postoperative pain is an essential component of the care and welfare of laboratory animals. A sustained-release formulation of buprenorphine (Bup-SR) has recently been introduced to the veterinary market and has been reported to provide analgesia for as long as 72 h. Using evoked mechanical and thermal hypersensitivity tests, we here evaluated the antinociceptive effects of Bup-SR in a model of incisional pain in rats. Paw withdrawal responses were obtained before and 1 through 4 d after surgery. Rats are assigned to receive Bup-SR (0.3, 1.2, or 4.5 mg/kg SC once) or buprenorphine HCl (Bup HCl, 0.05 mg/kg SC twice daily for 3 d). Responses to mechanical and thermal stimuli in the 1.2 and 4.5 Bup-SR groups did not differ from those of rats in the Bup HCl group. Thermal latency on day 3 in rats that received 0.3 mg/kg Bup-SR was significantly different from baseline, indicating that this dose effectively decreased thermal hypersensitivity for at least 48 h. Marked sedation occurred in rats in the 4.5 Bup-SR group. Our findings indicate that Bup-SR at 0.3 or 1.2 mg/kg SC is effective in minimizing hypersensitivity with minimal sedation for at least 48 h (thermal hypersensitivity) and 72 h, respectively, in the incisional pain model in rats.
authors.affiliation: Department of Comparative Medicine, Stanford University, Stanford, California, USA. hhchum@stanford.edu.
authors.forename: Helen H
authors.initials: HH
authors.lastname: Chum
authors.affiliation: Department of Mathematics, Naresuan University, Phitsanulok, Thailand.
authors.forename: Katechan
authors.initials: K
authors.lastname: Jampachairsri
authors.affiliation: Department of Laboratory Animal Resources, North Carolina State University, Raleigh, North Carolina, USA.
authors.forename: Gabriel P
authors.initials: GP
authors.lastname: McKeon
authors.affiliation: Department of Anesthesia, Stanford University, Stanford, California, USA.
authors.forename: David C
authors.initials: DC
authors.lastname: Yeomans
authors.affiliation: Department of Comparative Medicine, Stanford University, Stanford, California, USA.
authors.forename: Cholawat
authors.initials: C
authors.lastname: Pacharinsak
authors.affiliation: Department of Comparative Medicine, Stanford University, Stanford, California, USA.
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Felt
mesh_terms: D000701:Analgesics, Opioid / Q000008:administration & dosage*; D000827:Animal Welfare; D000818:Animals; D002047:Buprenorphine / Q000008:administration & dosage*; D003692:Delayed-Action Preparations; D004195:Disease Models, Animal; D008297:Male; D059408:Pain Management / Q000662:veterinary*; D010147:Pain Measurement / Q000187:drug effects* / Q000662:veterinary*; D010149:Postoperative Pain / Q000188:drug therapy* / Q000517:prevention & control / Q000662:veterinary*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000701:Analgesics, Opioid; D003692:Delayed-Action Preparations; D002047:Buprenorphine