title: Fluorescent analog of OSW-1 and its cellular localization.
abstract: OSW-1 is a steroidal saponin, which has emerged as an attractive anticancer agent with highly cancer cell selective activity. A fluorescent analog was prepared from the natural product to analyze its cellular uptake and localization. We found that the fluorescent analog is rapidly internalized into cells and is primarily distributed in endoplasmic reticulum and Golgi apparatus.
authors.affiliation: Tokyo University of Agriculture and Technology, School of Engineering, Koganei-shi, Tokyo 184-8588, Japan.
authors.forename: Rika
authors.initials: R
authors.lastname: Yamada
authors.affiliation: Tokyo University of Agriculture and Technology, School of Engineering, Koganei-shi, Tokyo 184-8588, Japan.
authors.forename: Tomoya
authors.initials: T
authors.lastname: Takeshita
authors.affiliation: Tokyo University of Agriculture and Technology, School of Engineering, Koganei-shi, Tokyo 184-8588, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Hiraizumi
authors.affiliation: Tokyo University of Agriculture and Technology, School of Engineering, Koganei-shi, Tokyo 184-8588, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Shinohe
authors.affiliation: Tokyo University of Agriculture and Technology, School of Engineering, Koganei-shi, Tokyo 184-8588, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ohta
authors.affiliation: Tokyo University of Agriculture and Technology, School of Engineering, Koganei-shi, Tokyo 184-8588, Japan. Electronic address: sakuraik@cc.tuat.ac.jp.
authors.forename: Kaori
authors.initials: K
authors.lastname: Sakurai
mesh_terms: D002783:Cholestenones / Q000737:chemistry / Q000493:pharmacokinetics*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry / Q000493:pharmacokinetics*; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D008968:Molecular Conformation; D012503:Saponins / Q000737:chemistry / Q000493:pharmacokinetics*; D013696:Temperature; D014018:Tissue Distribution
chemical_list: D002783:Cholestenones; D005456:Fluorescent Dyes; D012503:Saponins; C106408:OSW 1
keywords: Anticancer agent; Cellular localization; Cellular uptake; Fluorescent probe; Steroidal glycoside