title: Essential role of AWP1 in neural crest specification in Xenopus.
abstract: The neural crest (NC) comprises a transient and multipotent embryonic cell population, which gives rise to a wide variety of cell types, including craniofacial cartilage, melanocytes, and neurons and glia of the peripheral nervous system. The NC is induced by the integrated action of Wnt, FGF, and BMP signaling, and its cell fates are subsequently specified by a genetic cascade of specific transcription factors. Here we describe a critical role of AWP1 in NC induction during Xenopus early development. Xenopus AWP1 (XAWP1) was found to be expressed in the presumptive preplacodal ectoderm, neural tissue, and posterior dorsal mesoderm, but was absent in the neural fold along the anterior-posterior axis of the neurulae. Notably, XAWP1 was induced by FGF8a in naïve ectodermal tissue. XAWP1-depleted embryos exhibited defects in pigmentation, craniofacial cartilage, and in the dorsal fin. A knockdown of XAWP1 impaired both endogenous and the FGF8a or Wnt8-induced expression of NC markers without affecting mesoderm formation. Furthermore, NC induction inhibited by XAWP1 depletion was rescued by co-expression of activating forms of beta-catenin or TCF3. In addition, overexpression of XAWP1, in concert with BMP inhibition, induced the expression of neural plate border specifiers, Pax3 and Msx1, and these regulatory factors recovered NC induction in the XAWP1-depleted embryos. Beta-catenin stability and Wnt-responsive reporter activity were also impaired in AWP1-depleted cells. Taken together, these results suggest that XAWP1 functions as a mediator of Wnt signaling to regulate NC specification.
authors.affiliation: Department of Biomedical Sciences, Cell Dysfunction Research Center (CDRC). University of Ulsan College of Medicine, Pungnap-Dong, Songpa-Gu, Seoul, Republic of Korea.
authors.forename: Jeong-Han
authors.initials: JH
authors.lastname: Seo
authors.forename: Dong-Seok
authors.initials: DS
authors.lastname: Park
authors.forename: Mina
authors.initials: M
authors.lastname: Hong
authors.forename: Eun-Ju
authors.initials: EJ
authors.lastname: Chang
authors.forename: Sun-Cheol
authors.initials: SC
authors.lastname: Choi
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000502:physiology*; D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000502:physiology; D002356:Cartilage / Q000502:physiology; D019070:Cell Lineage; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D051957:MSX1 Transcription Factor / Q000502:physiology; D008648:Mesoderm / Q000502:physiology; D009432:Neural Crest / Q000196:embryology*; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors / Q000502:physiology; D015398:Signal Transduction; D058127:Transcription Factor 3 / Q000502:physiology; D051153:Wnt Proteins / Q000502:physiology; D029867:Xenopus Proteins / Q000502:physiology*; D014982:Xenopus laevis / Q000196:embryology* / Q000502:physiology; D051176:beta Catenin / Q000502:physiology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D019485:Bone Morphogenetic Proteins; D051957:MSX1 Transcription Factor; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors; C489411:Pax3 protein, Xenopus; C544917:Tcf3 protein, Xenopus; D058127:Transcription Factor 3; D051153:Wnt Proteins; D029867:Xenopus Proteins; C000593005:ZFand6 protein, Xenopus; D051176:beta Catenin