title: [Temporal and spacial expression of perlecan in dental pulp after injury].
abstract: OBJECTIVE
  To investigate the temporal and special expressions and functions of perlecan in dental pulp of rats after injury.
  
  
  METHODS
  Specimens of one side of maxillary molars of 24 male SD rats were collected 1,3,5,7 days after injury, and the other side were used as controls. The specimens were decalcified and embedded in paraffin, sliced by longitudinal section and stained by immunohistochemistry. The staining of perlecan and β1 integrin were examined, and the average optical density (AOD) was compared. The measurement data were analyzed by ANOVA using SPSS 19.0 software package.
  
  
  RESULTS
  The staining intensity of perlecan in dental pulp in the experimental group was significantly higher compared with that of the control group. The immunolocalization of perlecan in dental pulp was gradually progressed as the inflamed tissue was progressing after injury, and its AOD was significantly higher than that of control group (F=11.104,P>0.05), especially in the group of 1 d. The staining of β1 integrin was generally enhanced in the pulp 3 d after injury, and its AOD was significantly higher than that of control group (F=124.18,P>0.05).
  
  
  CONCLUSIONS
  Perlecan may play an important role in tissue defense mechanism after injury through regulating angiogenesis and inflammatory cell infiltration. Supported by Science and Technology Research Project of Department of Education of Zhejiang Province (20051196) and Wenzhou City (Y20100015).
authors.affiliation: Department of Pediatric Dentistry,School and Hospital of Stomatology, Wenzhou Medical University. Wenzhou 325027, Zhejiang Province, China.E-mail:f8_104@163.com.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Sun
authors.forename: Yi-huai
authors.initials: YH
authors.lastname: Pan
authors.forename: Chuan-tong
authors.initials: CT
authors.lastname: Liu
mesh_terms: D000818:Animals; D003782:Dental Pulp / Q000293:injuries*; D016326:Extracellular Matrix Proteins; D019812:Heparan Sulfate Proteoglycans; D008297:Male; D008963:Molar; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016326:Extracellular Matrix Proteins; D019812:Heparan Sulfate Proteoglycans; C071927:perlecan