title: Genetic instability is prevented by Mrc1-dependent spatio-temporal separation of replicative and repair activities of homologous recombination: homologous recombination tolerates replicative stress by Mrc1-regulated replication and repair activities operating at S and G2 in distinct subnuclear compartments.
abstract: Homologous recombination (HR) is required to protect and restart stressed replication forks. Paradoxically, the Mrc1 branch of the S phase checkpoints, which is activated by replicative stress, prevents HR repair at breaks and arrested forks. Indeed, the mechanisms underlying HR can threaten genome integrity if not properly regulated. Thus, understanding how cells avoid genetic instability associated with replicative stress, a hallmark of cancer, is still a challenge. Here I discuss recent results that support a model by which HR responds to replication stress through replicative and repair activities that operate at different stages of the cell cycle (S and G2, respectively) and in distinct subnuclear structures. Remarkably, the replication checkpoint appears to control this scenario by inhibiting the assembly of HR repair centers at stressed forks during S phase, thereby avoiding genetic instability.
authors.affiliation: Departamento de Biología Molecular, Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER), Consejo Superior de Investigaciones Científicas (CSIC), Seville, Spain.
authors.forename: Félix
authors.initials: F
authors.lastname: Prado
mesh_terms: D002451:Cell Compartmentation; D002453:Cell Cycle / Q000235:genetics*; D002467:Cell Nucleus / Q000235:genetics; D016922:Cellular Senescence / Q000235:genetics*; D004260:DNA Repair; D004261:DNA Replication; D016195:G2 Phase / Q000235:genetics; D042822:Genomic Instability; D059765:Homologous Recombination / Q000235:genetics*; D006801:Humans; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic / Q000378:metabolism*; D016196:S Phase / Q000235:genetics; D013997:Time Factors
chemical_list: C573543:MRC1 protein, human; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic
keywords: DNA checkpoints; Rad51; homologous recombination; repair centers; replication