title: Crystallization and preliminary X-ray diffraction analysis of the amidase domain of allophanate hydrolase from Pseudomonas sp. strain ADP.
abstract: The allophanate hydrolase from Pseudomonas sp. strain ADP was expressed and purified, and a tryptic digest fragment was subsequently identified, expressed and purified. This 50 kDa construct retained amidase activity and was crystallized. The crystals diffracted to 2.5 Å resolution and adopted space group P21, with unit-cell parameters a = 82.4, b = 179.2, c = 112.6 Å, β = 106.6°.
authors.affiliation: Ecosystem Sciences, CSIRO, GPO Box 1700, Canberra, ACT 2601, Australia.
authors.forename: Sahil
authors.initials: S
authors.lastname: Balotra
authors.affiliation: Materials, Science and Engineering, CSIRO, 343 Royal Parade, Parkville, VIC 3052, Australia.
authors.forename: Janet
authors.initials: J
authors.lastname: Newman
authors.affiliation: Ecosystem Sciences, CSIRO, GPO Box 1700, Canberra, ACT 2601, Australia.
authors.forename: Nigel G
authors.initials: NG
authors.lastname: French
authors.affiliation: Ecosystem Sciences, CSIRO, GPO Box 1700, Canberra, ACT 2601, Australia.
authors.forename: Lyndall J
authors.initials: LJ
authors.lastname: Briggs
authors.affiliation: Materials, Science and Engineering, CSIRO, 343 Royal Parade, Parkville, VIC 3052, Australia.
authors.forename: Thomas S
authors.initials: TS
authors.lastname: Peat
authors.affiliation: Ecosystem Sciences, CSIRO, GPO Box 1700, Canberra, ACT 2601, Australia.
authors.forename: Colin
authors.initials: C
authors.lastname: Scott
mesh_terms: D000492:Allophanate Hydrolase / Q000737:chemistry*; D000581:Amidohydrolases / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004795:Enzyme Stability; D059748:Proteolysis; D011549:Pseudomonas / Q000201:enzymology*; D020539:Sequence Analysis, Protein; D014357:Trypsin / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D014357:Trypsin; D000581:Amidohydrolases; C019665:amidase; D000492:Allophanate Hydrolase
keywords: AtzF; Pseudomonas sp. strain ADP; allphanate hydrolase; amidase domain