title: Cinobufacin suppresses cell proliferation via miR-494 in BGC- 823 gastric cancer cells.
abstract: Cinobufacin is used clinically to treat patients with many solid malignant tumors. However, the mechanisms underlying action remain to be detailed. Our study focused on miRNAs involved in cinobufacin inhibition of GC cell proliferation. miRNA microarray analysis and real time PCR identified miR-494 as a significant cinobufacin- associated miRNA. In vivo, ectopic expression of miR-494 inhibited the proliferation and induced apoptosis of BGC-823 cells on CCK-8 and flow cytometry analysis. Further study verified BAG-1 (anti-apoptosis gene) to bea target of miR-494 by luciferase reporter assay and Western blotting. In summary, our study demonstrated that cinobufacin may inhibit the proliferation and promote the apoptosis of BGC-823 cells. Cinobufacin-associated miR-494 may indirectly be involved in cell proliferation and apoptosis by targeting BAG-1, pointing to use as a potential molecular target of cinobufacin in gastric cancer therapy.
authors.affiliation: First College of Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing, China E-mail : pinger_523@126.com.
authors.forename: Rong-Ping
authors.initials: RP
authors.lastname: Zhou
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.forename: Zhi-Li
authors.initials: ZL
authors.lastname: Shen
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Pan
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000664:Amphibian Venoms / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008516:Medicine, Chinese Traditional; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D012844:Sincalide; D013274:Stomach Neoplasms / Q000188:drug therapy; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; D000664:Amphibian Venoms; D000970:Antineoplastic Agents; C091732:BCL2-associated athanogene 1 protein; D004268:DNA-Binding Proteins; C547168:MIRN494 microRNA, human; D035683:MicroRNAs; D014157:Transcription Factors; C545813:huachansu; D012844:Sincalide