title: Genomic sequence analysis of the MHC class I G/F segment in common marmoset (Callithrix jacchus).
abstract: The common marmoset (Callithrix jacchus) is a New World monkey that is used frequently as a model for various human diseases. However, detailed knowledge about the MHC is still lacking. In this study, we sequenced and annotated a total of 854 kb of the common marmoset MHC region that corresponds to the HLA-A/G/F segment (Caja-G/F) between the Caja-G1 and RNF39 genes. The sequenced region contains 19 MHC class I genes, of which 14 are of the MHC-G (Caja-G) type, and 5 are of the MHC-F (Caja-F) type. Six putatively functional Caja-G and Caja-F genes (Caja-G1, Caja-G3, Caja-G7, Caja-G12, Caja-G13, and Caja-F4), 13 pseudogenes related either to Caja-G or Caja-F, three non-MHC genes (ZNRD1, PPPIR11, and RNF39), two miscRNA genes (ZNRD1-AS1 and HCG8), and one non-MHC pseudogene (ETF1P1) were identified. Phylogenetic analysis suggests segmental duplications of units consisting of basically five (four Caja-G and one Caja-F) MHC class I genes, with subsequent expansion/deletion of genes. A similar genomic organization of the Caja-G/F segment has not been observed in catarrhine primates, indicating that this genomic segment was formed in New World monkeys after the split of New World and Old World monkeys.
authors.affiliation: Division of Basic Medical Science and Molecular Medicine, Department of Molecular Life Science, Tokai University School of Medicine, Isehara, Kanagawa 259-1143, Japan;
authors.forename: Azumi
authors.initials: A
authors.lastname: Kono
authors.forename: Markus
authors.initials: M
authors.lastname: Brameier
authors.forename: Christian
authors.initials: C
authors.lastname: Roos
authors.forename: Shingo
authors.initials: S
authors.lastname: Suzuki
authors.forename: Atsuko
authors.initials: A
authors.lastname: Shigenari
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Kametani
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Kitaura
authors.forename: Takaji
authors.initials: T
authors.lastname: Matsutani
authors.forename: Ryuji
authors.initials: R
authors.lastname: Suzuki
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Inoko
authors.forename: Lutz
authors.initials: L
authors.lastname: Walter
authors.forename: Takashi
authors.initials: T
authors.lastname: Shiina
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002144:Callithrix / Q000235:genetics / Q000276:immunology*; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D020451:Contig Mapping; D023061:Gene Order; D016678:Genome / Q000235:genetics / Q000276:immunology*; D015698:Genomic Library; D023281:Genomics / Q000379:methods*; D015395:Histocompatibility Antigens Class I / Q000145:classification / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D011544:Pseudogenes / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D015395:Histocompatibility Antigens Class I