title: Potentially immunogenic proteins expressed similarly in human embryonic stem cells and induced pluripotent stem cells.
abstract: A major limitation of the use of cellular therapies is the loss of donor-derived cells because of immune incompatibility. While induced pluripotent stem (iPS) cells offer the potential for autologous transplant therapies, questions have been raised using a mouse model that specific antigens mediate the rejection of grafts after syngeneic transplants with iPS, but not embryonic stem (ES) cells. In this study, we examined whether the human homologs of these markers, HORMAD1, ZG16, and Cyp3A, are differentially expressed in human iPS versus ES cells, as well as undifferentiated and in vitro-differentiated cells. Both qRT-PCR and flow cytometric analyses demonstrated similar gene and protein expression profiles for iPS and ES cells regardless of differentiation state. Our data are consistent with a recent study in mice that showed no evidence of rejection of differentiated syngeneic iPS cells. Furthermore, our results suggest that expression of these gene products cannot predict differences in clinical outcomes between human iPS and ES-derived cells.
authors.affiliation: Stem Cell Institute, Division of Endocrinology, Department of Medicine, Schulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, MN 55455, USA.
authors.forename: Kristen M
authors.initials: KM
authors.lastname: Maynard
authors.forename: Upasana
authors.initials: U
authors.lastname: Arvindam
authors.forename: Madeline
authors.initials: M
authors.lastname: Cross
authors.forename: Meri T
authors.initials: MT
authors.lastname: Firpo
mesh_terms: D018797:Cell Cycle Proteins / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D051544:Cytochrome P-450 CYP3A / Q000378:metabolism*; D053595:Embryonic Stem Cells / Q000378:metabolism*; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D018797:Cell Cycle Proteins; C576915:HORMAD1 protein, human; D037102:Lectins; C519401:ZG16 protein, human; C104464:CYP3A protein, human; D051544:Cytochrome P-450 CYP3A
keywords: Embryonic stem cells; immune rejection; induced pluripotent stem cells; transplant therapy; tumor antigens