title: Rapid monitoring of glycerol in fermentation growth media: Facilitating crude glycerol bioprocess development.
abstract: Recently, the need for crude glycerol valorisation from the biodiesel industry has generated many studies for practical and economic applications. Amongst them, fermentations based on glycerol media for the production of high value metabolites are prominent applications. This has generated a need to develop analytical techniques which allow fast and simple glycerol monitoring during fermentation. The methodology should be fast and inexpensive to be adopted in research, as well as in industrial applications. In this study three different methods were analysed and compared: two common methodologies based on liquid chromatography and enzymatic kits, and the new method based on a DotBlot assay coupled with image analysis. The new methodology is faster and cheaper than the other conventional methods, with comparable performance. Good linearity, precision and accuracy were achieved in the lower range (10 or 15 g/L to depletion), the most common range of glycerol concentrations to monitor fermentations in terms of growth kinetics.
authors.affiliation: Bioengineering Department, Institut Químic de Sarrià (IQS), Universitat Ramon Llull. Via Augusta 390, 08017 Barcelona, Spain.
authors.forename: Sergi
authors.initials: S
authors.lastname: Abad
authors.affiliation: Bioengineering Department, Institut Químic de Sarrià (IQS), Universitat Ramon Llull. Via Augusta 390, 08017 Barcelona, Spain.
authors.forename: Xavier
authors.initials: X
authors.lastname: Pérez
authors.affiliation: Bioengineering Department, Institut Químic de Sarrià (IQS), Universitat Ramon Llull. Via Augusta 390, 08017 Barcelona, Spain.
authors.forename: Antoni
authors.initials: A
authors.lastname: Planas
authors.affiliation: Bioengineering Department, Institut Químic de Sarrià (IQS), Universitat Ramon Llull. Via Augusta 390, 08017 Barcelona, Spain. Electronic address: xavier.turon@iqs.edu.
authors.forename: Xavier
authors.initials: X
authors.lastname: Turon
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism*
chemical_list: D003470:Culture Media; D005990:Glycerol
keywords: Bioprocess; Fermentation; Glycerol; HPLC; Monitoring; TLC