title: Measure for measure: determining, inferring and guessing auxin gradients at the root tip.
abstract: The plant hormone auxin is transported from sites of synthesis to sites of action. Auxin responses are mediated by fast (non-transcriptional) and slow (transcriptional; ubiquitinylation) responses, which affect physiological changes at cellular and organismal scales. As such, auxin transport vectors regulate programmed and plastic growth responses to optimize growth and development. Here we address some common problems in extrapolating 'universal' understanding of auxin transport streams from analyses of loss-of-function mutants and auxin transport inhibitors. We also discuss the analytical methods and tools used to directly quantify, measure and infer auxin gradients within the plant [DR5:GUS/GFP (beta-glucuronidase/green fluorescent protein), DII-VENUS; surface electrodes, direct quantification]. We discuss the assumptions and limitations of each of these analyses, present comparative summaries of auxin transport methods and assay conditions (diffusion, non-specific transport and relevant assay conditions), and consider what is actually being transported and measured [labeled-indole-3-acetic acid (IAA), IAA metabolites].
authors.affiliation: Department of Environmental Science and Technology, University of Maryland, College Park, MD, 20742, USA; Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, 20742, USA.
authors.forename: Wendy Ann
authors.initials: WA
authors.lastname: Peer
authors.forename: Mark K
authors.initials: MK
authors.lastname: Jenness
authors.forename: Angus S
authors.initials: AS
authors.lastname: Murphy
mesh_terms: D001692:Biological Transport / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D007210:Indoleacetic Acids / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D010940:Plant Proteins