title: [Detection of allergenic substances (shrimp, crab) in processed seafood].
abstract: We have carried out a study (2009-2012) on processed seafood products in order to determine the level of contamination with shrimp and crab. In 2010-2012, after the Allergy Labeling Regulation went into effect, the detection rate of crustacean protein in processed seafood products including small fish, such as niboshi, tukudani and so on (both boiled and dried), was 63%. Detection rates for processed seafood products in which crustacean protein levels were below 1 μg/g were 36% with and 58% without advisory labels, allowing us to conclude that 60% of labels were adequate. On the other hand, the detection rate for processed seafood products with crustacean protein levels higher than the baseline of 10 μg/g was 9%, of which 60% carried no advisory labels. The rate of shrimp DNA detection using the Akiami primer in processed foods containing shrimp and crab was high (73%). This suggests that it is necessary to test these products using the Akiami primer for supplemental analyses of shrimp DNA. The PCR analysis for crab DNA detection failed due to combined detection of mantis shrimp DNA, which accounted for 8% of the total detected.
authors.affiliation: Kanagawa Prefectual Institute of Public Health.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Watanabe
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Saita
authors.forename: Chie
authors.initials: C
authors.lastname: Akaboshi
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Ohsawa
authors.forename: Shigeki
authors.initials: S
authors.lastname: Hashiguchi
authors.forename: Maki
authors.initials: M
authors.lastname: Miyazawa
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000302:isolation & purification*; D003445:Crustacea; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D005512:Food Hypersensitivity / Q000517:prevention & control*; D005515:Food Labeling / Q000331:legislation & jurisprudence*; D007881:Legislation, Food; D016133:Polymerase Chain Reaction / Q000379:methods*; D017747:Seafood / Q000032:analysis*
chemical_list: D060829:Arthropod Proteins