title: [MIP-1α promotes the migration ability of Jurkat cell through human brain microvascular endothelial cell monolayer].
abstract: This study was purposed to explore the mechanism of central nervous system (CNS) leukemia resulting from brain metastasis of human acute T-cell leukemia (T-ALL) cells and the role of MIP-1α in migration of Jurkat cells through human brain microvascular endothelial cells (HBMEC). The real-time PCR, siRNA test, transendothelial migration test, endothelial permeability assay and cell adhesion assay were used to detect MIP-1α expression, penetration and migration ability as well as adhesion capability respectively. The results showed that the MIP-1α expression in Jurkat cells was higher than that in normal T cells and CCRF-HSB2, CCRF-CEM , SUP-T1 cells. The MIP-1α secreted from Jurkat cells enhanced the ability of Jurkat cells to penetrate through HBMEC, the ability of Jurkat cells treated by MIP-1α siRNA to adhere to HBMEC and to migrate trans endothelial cells decreased. It is concluded that the MIP-1α secreted from Jurkat cells participates in process of penetrating the Jurkat cells through HBMEC monolayer.
authors.affiliation: Department of Blood Transfusion, The First Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
authors.forename: Yi-Ran
authors.initials: YR
authors.lastname: Ma
authors.affiliation: Department of Blood Transfusion, The First Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Blood Transfusion, The First Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Blood Transfusion, The First Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
authors.forename: Yi-Yang
authors.initials: YY
authors.lastname: Liu
authors.affiliation: Department of Blood Transfusion, The First Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Song
authors.affiliation: Department of Blood Transfusion, The First Hospital of China Medical University, Shenyang 110001, Liaoning Province, China. E-mail: hyw82666@163.com.
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Hao
mesh_terms: D001932:Brain Neoplasms / Q000473:pathology* / Q000556:secondary; D002448:Cell Adhesion; D002465:Cell Movement; D054405:Chemokine CCL3 / Q000378:metabolism*; D042783:Endothelial Cells / Q000473:pathology*; D004730:Endothelium, Vascular / Q000473:pathology; D006801:Humans; D019169:Jurkat Cells; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Q000473:pathology*
chemical_list: C515429:CCL3 protein, human; D054405:Chemokine CCL3