title: Multifunctional adhesive silk fibroin with blending of RGD-bioconjugated mussel adhesive protein.
abstract: Silk has recently been exploited in various fields due to its superior mechanical properties. However, this material's lack of biological functions and relatively poor biodegradation have hindered its wide use in applications related to cells and tissues. Here, we improved the overall characteristics of silkworm silk fibroin (SF) by introduction of RGD peptide-fused recombinant mussel adhesive protein (MAP-RGD). Simple blending of MAP-RGD provided not only bulk-scale adhesive ability but also microscale adhesiveness to cells and various biomolecules. MAP-RGD-blended SF fibers supported enhanced adhesion, proliferation, and spreading of mammalian cells as well as the efficient attachment of biomolecules, including carbohydrate and protein. In addition, the hydrophilicity, swelling, and biodegradability of the MAP-RGD-blended SF material were improved without notable hampering of the original mechanical properties of SF. Therefore, the adhesive silk fibrous scaffold could be successfully used in diverse biomedical engineering applications.
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology , Pohang 790-784, Korea.
authors.forename: Yun Jung
authors.initials: YJ
authors.lastname: Yang
authors.forename: Yunkyeoung
authors.initials: Y
authors.lastname: Kwon
authors.forename: Bong-Hyuk
authors.initials: BH
authors.lastname: Choi
authors.forename: Dooyup
authors.initials: D
authors.lastname: Jung
authors.forename: Jeong Hyun
authors.initials: JH
authors.lastname: Seo
authors.forename: Ki Hoon
authors.initials: KH
authors.lastname: Lee
authors.forename: Hyung Joon
authors.initials: HJ
authors.lastname: Cha
mesh_terms: D000269:Adhesives / Q000737:chemistry; D001672:Biocompatible Materials; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D005349:Fibroins / Q000737:chemistry*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology; D009842:Oligopeptides / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology; D011506:Proteins / Q000737:chemistry*; D011993:Recombinant Fusion Proteins / Q000737:chemistry*; D054457:Tissue Scaffolds
chemical_list: D000269:Adhesives; D001672:Biocompatible Materials; D009842:Oligopeptides; D011506:Proteins; D011993:Recombinant Fusion Proteins; C037403:adhesive protein, mussel; C482288:fibroin, silkworm; C047981:arginyl-glycyl-aspartic acid; D005349:Fibroins