title: Aldehyde dehydrogenase-bright cells correlated with the colony-forming unit-granulocyte-macrophage assay of thawed cord blood units.
abstract: BACKGROUND
  The number of aldehyde dehydrogenase-bright (ALDH(br) ) cells has been suggested as a viable marker of hematopoietic stem cell function. We evaluated the suitability of ALDH(br) cell analysis in the quality assessment of postthaw cord blood (CB) units.
  
  
  METHODS
  A total of 245 CB units were obtained for estimating the numbers of total nucleated cells (TNCs), CD34+ cells, ALDH(br) cells, ALDH(br) cells among CD34+ cells (CD34+ALDH(br) cells), CD34+ cells among ALDH(br) cells (ALDH(br) CD34+ cells), colony-forming unit (CFU)-granulocyte-macrophages (GMs), and CFU-granulocyte-erythrocyte-macrophage-megakaryocytes (GEMMs). Simple linear regression analysis was performed to assess the correlation between the number of TNCs and CD34+ cells before and after crypreservation and CD34+ALDH(br) cells, ALDH(br) cells, and ALDH(br) CD34+ cells after cryopreservation and the number of CFU-GEMMS and CFU-GMs.
  
  
  RESULTS
  The number of CFU-GMs was found to be significantly correlated with the number of CD34+ cells before and after cryopreservation (r = 0.418 and r = 0.359, respectively), CD34+ALDH(br) cells, ALDH(br) cells, and ALDH(br) CD34+ cells (r = 0.426, r = 0.455, and r = 0.469, respectively). The number of CFU-GEMMs was found to be significantly correlated with the number of TNCs and CD34+ cells before and after cryopreservation (TNCs, r = 0.251 and r = 0.250, respectively; CD34+ cells, r = 0.391 and r = 0.347, respectively), CD34+ALDH(br) cells, ALDH(br) cells, and ALDH(br) CD34+ cells (r = 0.297, r = 0.297, and r = 0.252, respectively).
  
  
  CONCLUSIONS
  The high correlation found between ALDH activity and CFU-GM number supports the suitability of ALDH analysis in the quality assessment of postthaw CB units.
authors.affiliation: Department of Laboratory Medicine, Gyeongsang National University Hospital, Jinju, Korea.
authors.forename: Hye Ryun
authors.initials: HR
authors.lastname: Lee
authors.forename: Sue
authors.initials: S
authors.lastname: Shin
authors.forename: Jong Hyun
authors.initials: JH
authors.lastname: Yoon
authors.forename: Eun Youn
authors.initials: EY
authors.lastname: Roh
authors.forename: Byoung Jae
authors.initials: BJ
authors.lastname: Kim
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Song
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D001772:Blood Cell Count; D001793:Blood Preservation / Q000009:adverse effects / Q000379:methods; D003114:Colony-Forming Units Assay / Q000592:standards*; D036101:Cord Blood Stem Cell Transplantation; D015925:Cryopreservation; D005312:Fetal Blood / Q000166:cytology* / Q000201:enzymology*; D005615:Freezing; D055014:Granulocyte-Macrophage Progenitor Cells / Q000201:enzymology*; D006412:Hematopoietic Stem Cells / Q000201:enzymology; D006801:Humans
chemical_list: D015415:Biomarkers; D000444:Aldehyde Dehydrogenase