title: Complete mitochondrial genome sequence of a phytophagous ladybird beetle, Henosepilachna pusillanima (Mulsant) (Coleoptera: Coccinellidae).
abstract: Ladybird beetles are generally considered as agriculturally beneficial insects, but the ladybird beetles in the coleopteran subfamily Epilachninae are phytophagous and major plant feeding pest species which causes severe economic losses to cucurbitaceous and solanaceous crops. Henosepilachna pusillanima (Mulsant) is one of the important pest species of ladybird beetle. In this report, we sequenced and characterized the complete mitochondrial genome of H. pusillanima. For sequencing of the complete mitochondrial genome, we used the Ion Torrent sequencing platform. The complete circular mitochondrial genome of the H. pusillanima was determined to be 16,216 bp long. There were totally 13 protein coding genes, 22 transfer RNA, 2 ribosomal RNA and a control (A + T-rich) region estimated to be 1690 bp. The gene arrangement and orientations of assembled mitogenome were identical to the reported predatory ladybird beetle Coccinella septempunctata L. This is the first completely sequenced coleopteran mitochondrial genome from the beetle subfamily Epilachninae from India. Data generated in this study will benefit future comparative genomics studies for understanding the evolutionary relationships between predatory and phytophagous coccinellid beetles.
authors.affiliation: a Division of Crop Improvement (Entomology) , ICAR Research Complex for NEH Region , Umroi Road , Umiam (Barapani) , Meghalaya , India and.
authors.forename: G T
authors.initials: GT
authors.lastname: Behere
authors.affiliation: a Division of Crop Improvement (Entomology) , ICAR Research Complex for NEH Region , Umroi Road , Umiam (Barapani) , Meghalaya , India and.
authors.forename: D M
authors.initials: DM
authors.lastname: Firake
authors.affiliation: b Biosecurity Flagship, CSIRO Ecosystem Sciences, Black Mountain Laboratories , Canberra , Australia.
authors.forename: W T
authors.initials: WT
authors.lastname: Tay
authors.affiliation: a Division of Crop Improvement (Entomology) , ICAR Research Complex for NEH Region , Umroi Road , Umiam (Barapani) , Meghalaya , India and.
authors.forename: N S
authors.initials: NS
authors.lastname: Azad Thakur
authors.affiliation: a Division of Crop Improvement (Entomology) , ICAR Research Complex for NEH Region , Umroi Road , Umiam (Barapani) , Meghalaya , India and.
authors.forename: S V
authors.initials: SV
authors.lastname: Ngachan
mesh_terms: D000818:Animals; D001483:Base Sequence; D001517:Coleoptera / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D049750:Genome, Insect; D054629:Genome, Mitochondrial; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D004272:DNA, Mitochondrial
keywords: Complete sequence; Henosepilachna pusillanima; mitogenome