title: Osthole suppresses the migratory ability of human glioblastoma multiforme cells via inhibition of focal adhesion kinase-mediated matrix metalloproteinase-13 expression.
abstract: Glioblastoma multiforme (GBM) is the most common type of primary and malignant tumor occurring in the adult central nervous system. GBM often invades surrounding regions of the brain during its early stages, making successful treatment difficult. Osthole, an active constituent isolated from the dried C. monnieri fruit, has been shown to suppress tumor migration and invasion. However, the effects of osthole in human GBM are largely unknown. Focal adhesion kinase (FAK) is important for the metastasis of cancer cells. Results from this study show that osthole can not only induce cell death but also inhibit phosphorylation of FAK in human GBM cells. Results from this study show that incubating GBM cells with osthole reduces matrix metalloproteinase (MMP)-13 expression and cell motility, as assessed by cell transwell and wound healing assays. This study also provides evidence supporting the potential of osthole in reducing FAK activation, MMP-13 expression, and cell motility in human GBM cells.
authors.affiliation: Department of Biotechnology, Asia University, Taichung 41354, Taiwan. tsaicf@asia.edu.tw.
authors.forename: Cheng-Fang
authors.initials: CF
authors.lastname: Tsai
authors.affiliation: Department of Cell and Tissue Engineering, Changhua Christian Hospital, Changhua 50006, Taiwan. ibizayeh0816@hotmail.com.
authors.forename: Wei-Lan
authors.initials: WL
authors.lastname: Yeh
authors.affiliation: Department of General Surgery, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Taichung 42743, Taiwan. guns5150@ms27.hinet.net.
authors.forename: Jia-Hong
authors.initials: JH
authors.lastname: Chen
authors.affiliation: Department of Physiology, School of Medicine, China Medical University, Taichung 40402, Taiwan. clin33@mail.cmu.edu.tw.
authors.forename: Chingju
authors.initials: C
authors.lastname: Lin
authors.affiliation: Department of Pharmacology and Institute of Medicine, College of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan. sshuang@csmu.edu.tw.
authors.forename: Shiang-Suo
authors.initials: SS
authors.lastname: Huang
authors.affiliation: Graduate Institute of Neural and Cognitive Sciences, China Medical University, Taichung 40402, Taiwan. dahyuu@mail.cmu.edu.tw.
authors.forename: Dah-Yuu
authors.initials: DY
authors.lastname: Lu
mesh_terms: D015153:Blotting, Western; D001932:Brain Neoplasms / Q000201:enzymology / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D031048:Cnidium / Q000737:chemistry; D003374:Coumarins / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism*; D005638:Fruit / Q000737:chemistry; D005909:Glioblastoma / Q000201:enzymology / Q000473:pathology; D006801:Humans; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D014443:Tyrosine / Q000378:metabolism
chemical_list: D003374:Coumarins; D014443:Tyrosine; D051416:Focal Adhesion Protein-Tyrosine Kinases; D053509:Matrix Metalloproteinase 13; C046627:osthol