title: [Relationship between DEK oncogene expression and oral squamous cell carcinoma].
abstract: OBJECTIVE
  To investigate the expression of gene DEK in oral squamous cell carcinoma, and explore the role of DEK in the development and metastasis of oral squamous cell carcinoma.
  
  
  METHODS
  Thirty-three specimens of oral squamous cell carcinoma (OSCC), 28 specimens of cervical lymph nodes metastasis, and 33 specimens of normal oral mucosal tissues adjacent to the cancerous lesions were examined. Polymerase chain reaction with reverse transcription (RT-PCR) and immunohistochemistry were used to detect mRNA and protein expression of DEK, respectively.The results were analysed with SPSS17.0 software package.
  
  
  RESULTS
  RT-PCR and immunohistochemistry showed that the expression of DEK mRNA and DEK protein in metastatic lymph nodes (82.1%, 82.1%) and cancerous tissues (81.2%, 76.8%) of OSCC were significantly higher than in the adjacent tissues (27.3%, 18.2%). The difference between cancerous tissues and adjacent tissues was significant (P<0.05).
  
  
  CONCLUSIONS
  DEK may play an important role in the occurrence and metastasis of OSCC.
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Stomatology, China Medical University, Liaoning Research Institute of Stomatology. Shenyang 110002;China.E-mail:wangxuanstepanie@163.com.
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
authors.forename: Shao-hui
authors.initials: SH
authors.lastname: Huang
authors.forename: Li-qiao
authors.initials: LQ
authors.lastname: Yu
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Yao
authors.initials: Y
authors.lastname: Diao
authors.forename: Chang-fu
authors.initials: CF
authors.lastname: Sun
mesh_terms: D002294:Carcinoma, Squamous Cell; D002868:Chromosomal Proteins, Non-Histone / Q000096:biosynthesis*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D009061:Mouth Mucosa; D009062:Mouth Neoplasms; D009333:Neck; D015513:Oncogene Proteins / Q000096:biosynthesis*; D000075223:Poly-ADP-Ribose Binding Proteins; D012333:RNA, Messenger
chemical_list: D002868:Chromosomal Proteins, Non-Histone; C084738:DEK protein, human; D015513:Oncogene Proteins; D000075223:Poly-ADP-Ribose Binding Proteins; D012333:RNA, Messenger