title: A polymorphism in melanoma differentiation-associated gene 5 may be a risk factor for enterovirus 71 infection.
abstract: Enterovirus 71 (EV71) infection has a wide variety of clinical manifestations, from no symptoms to fatal disease. Host immune response may be a determinant of disease severity. We investigated the association of polymorphisms in three pattern recognition receptor (PRR) genes-toll-like receptor 3 (TLR3) (rs3775291), retinoic acid-inducible gene I (RIG-I) (rs10813831) and melanoma differentiation-associated gene 5 (MDA5) (rs1990760)-with the severity of EV71 infection. Polymorphisms of candidate genes in 87 EV71-infected patients and 57 asymptomatic controls were detected. Binary logistic regression analysis revealed statistically significant differences in polymorphism of MDA5 (rs1990760) between patients with severe EV71 infection and asymptomatic controls in an additive model (OR 0.424, 95% CI 0.213-0.845, p 0.015) and a dominant model (OR 0.256, 95% CI 0.103-0.635, p 0.003). Polymorphism of MDA5 (rs1990760) (OR 0.399, 95% CI 0.199-0.798, p 0.009) was found to be associated with the severity of EV71 infection with the analysis of ordinal logistic regression. These results indicated the association between MDA5 (rs1990760) polymorphism and an increased risk of a severe EV71 infection in Chinese children, which offers potential for investigating the innate immune mechanism of EV71 infection and identifying at-risk infants, for whom a preventive strategy may reduce the severity of EV71 infection.
authors.affiliation: National Institute for Viral Disease Control and Prevention, China CDC, Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing, China; Ji'nan Municipal Centre for Disease Control and Prevention, Ji'nan, China.
authors.forename: L
authors.initials: L
authors.lastname: Pang
authors.forename: X
authors.initials: X
authors.lastname: Gong
authors.forename: N
authors.initials: N
authors.lastname: Liu
authors.forename: G
authors.initials: G
authors.lastname: Xie
authors.forename: W
authors.initials: W
authors.lastname: Gao
authors.forename: G
authors.initials: G
authors.lastname: Kong
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Jin
authors.forename: Z
authors.initials: Z
authors.lastname: Duan
mesh_terms: D002675:Child, Preschool; D002681:China; D053487:DEAD-box RNA Helicases / Q000235:genetics*; D029821:Enterovirus A, Human / Q000502:physiology*; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006232:Hand, Foot and Mouth Disease / Q000097:blood / Q000235:genetics* / Q000821:virology*; D006801:Humans; D007223:Infant; D007297:Inpatients; D000072640:Interferon-Induced Helicase, IFIH1; D016015:Logistic Models; D008297:Male; D020641:Polymorphism, Single Nucleotide; D018168:Receptors, Retinoic Acid / Q000235:genetics; D051196:Toll-Like Receptor 3 / Q000235:genetics
chemical_list: C437968:PLAAT4 protein, human; D018168:Receptors, Retinoic Acid; C495344:TLR3 protein, human; D051196:Toll-Like Receptor 3; C452815:IFIH1 protein, human; D053487:DEAD-box RNA Helicases; D000072640:Interferon-Induced Helicase, IFIH1
keywords: Enterovirus 71 infection; MDA5; RIG-I; TLR3; single-nucleotide polymorphism