title: Genome-wide re-sequencing of multidrug-resistant Mycobacterium leprae Airaku-3.
abstract: Genotyping and molecular characterization of drug resistance mechanisms in Mycobacterium leprae enables disease transmission and drug resistance trends to be monitored. In the present study, we performed genome-wide analysis of Airaku-3, a multidrug-resistant strain with an unknown mechanism of resistance to rifampicin. We identified 12 unique non-synonymous single-nucleotide polymorphisms (SNPs) including two in the transporter-encoding ctpC and ctpI genes. In addition, two SNPs were found that improve the resolution of SNP-based genotyping, particularly for Venezuelan and South East Asian strains of M. leprae.
authors.affiliation: Global Health Institute, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
authors.forename: P
authors.initials: P
authors.lastname: Singh
authors.forename: A
authors.initials: A
authors.lastname: Benjak
authors.forename: S
authors.initials: S
authors.lastname: Carat
authors.forename: M
authors.initials: M
authors.lastname: Kai
authors.forename: P
authors.initials: P
authors.lastname: Busso
authors.forename: C
authors.initials: C
authors.lastname: Avanzi
authors.forename: A
authors.initials: A
authors.lastname: Paniz-Mondolfi
authors.forename: C
authors.initials: C
authors.lastname: Peter
authors.forename: K
authors.initials: K
authors.lastname: Harshman
authors.forename: J
authors.initials: J
authors.lastname: Rougemont
authors.forename: M
authors.initials: M
authors.lastname: Matsuoka
authors.forename: S T
authors.initials: ST
authors.lastname: Cole
mesh_terms: D001210:Asia, Southeastern; D024901:Drug Resistance, Multiple, Bacterial; D016680:Genome, Bacterial; D005838:Genotype; D006801:Humans; D007918:Leprosy / Q000382:microbiology; D008969:Molecular Sequence Data; D009166:Mycobacterium leprae / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA / Q000379:methods*; D014687:Venezuela
keywords: Leprosy; Mycobacterium leprae; molecular epidemiology; rifampicin-resistance; single nucleotide polymorphism