title: Facile and quantitative electrochemical detection of yeast cell apoptosis.
abstract: An electrochemical method based on square wave anodic stripping voltammetry (SWASV) was developed to detect the apoptosis of yeast cells conveniently and quantitatively through the high affinity between Cu(2+) and phosphatidylserine (PS) translocated from the inner to the outer plasma membrane of the apoptotic cells. The combination of negatively charged PS and Cu(2+) could decrease the electrochemical response of Cu(2+) on the electrode. The results showed that the apoptotic rates of cells could be detected quantitatively through the variations of peak currents of Cu(2+) by SWASV, and agreed well with those obtained through traditional flow cytometry detection. This work thus may provide a novel, simple, immediate and accurate detection method for cell apoptosis.
authors.affiliation: 1] School of Life Sciences, Anhui Agricultural University, Hefei 230036, People's Republic of China [2].
authors.forename: Qiulin
authors.initials: Q
authors.lastname: Yue
authors.affiliation: 1] Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, People's Republic of China [2].
authors.forename: Shiquan
authors.initials: S
authors.lastname: Xiong
authors.affiliation: Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, People's Republic of China.
authors.forename: Dongqing
authors.initials: D
authors.lastname: Cai
authors.affiliation: Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, People's Republic of China.
authors.forename: Zhengyan
authors.initials: Z
authors.lastname: Wu
authors.affiliation: School of Life Sciences, Anhui Agricultural University, Hefei 230036, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis; D003300:Copper / Q000737:chemistry; D055664:Electrochemical Techniques; D004563:Electrochemistry / Q000379:methods; D005434:Flow Cytometry; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000378:metabolism; D015003:Yeasts / Q000737:chemistry* / Q000378:metabolism
chemical_list: D003300:Copper