title: N-myc downstream regulated gene 1 acts as a tumor suppressor in ovarian cancer.
abstract: Although implicated in a number of tumor types, the role of N-myc downstream regulated gene 1 (NDRG1) in ovarian cancer (OC) is unclear. In the present study, we used short hairpin RNA (shRNA) to silence NDRG1 in the OC cell line OVCAR3 and assessed the effect of its knockdown on cell morphology, proliferation, colony formation, migration and invasion. To complement these knockdown studies, we overexpressed NDRG1 in the same cell line. We found that NDRG1 knockdown significantly enhanced OVCAR3 proliferation, migration and invasion; however, there were no apparent changes in cell morphology. We also examined the effect in vivo and found that NDRG1 depletion promoted OVCAR3 xenograft growth in nude mice. In accordance with these data, we found that NDRG1 overexpression decreased proliferation, adhesion and apoptosis, and induced G0/G1 cell cycle arrest in OVCAR3 cells; expression of p21 and p53 was also increased. In conclusion, we demonstrated that NDRG1 acts as a tumor suppressor in ovarian carcinogenesis and may be a potential therapeutic target in this disease.
authors.affiliation: Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China.
authors.forename: Bei
authors.initials: B
authors.lastname: Wang
authors.affiliation: Hebei General Hospital, Shijiazhuang, Hebei 050051, P.R. China.
authors.forename: Jianli
authors.initials: J
authors.lastname: Li
authors.affiliation: Hebei General Hospital, Shijiazhuang, Hebei 050051, P.R. China.
authors.forename: Zhanying
authors.initials: Z
authors.lastname: Ye
authors.affiliation: Hebei General Hospital, Shijiazhuang, Hebei 050051, P.R. China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Li
authors.affiliation: Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Wu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D002448:Cell Adhesion / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000096:biosynthesis; D005260:Female; D059585:G1 Phase Cell Cycle Checkpoints / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness / Q000235:genetics*; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000473:pathology*; D034622:RNA Interference; D034741:RNA, Small Interfering; D014183:Transplantation, Heterologous; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis; D000099259:N-myc Downstream-Regulated Gene 1 Protein
chemical_list: D018797:Cell Cycle Proteins; D050759:Cyclin-Dependent Kinase Inhibitor p21; D047908:Intracellular Signaling Peptides and Proteins; D034741:RNA, Small Interfering; D016159:Tumor Suppressor Protein p53; D000099259:N-myc Downstream-Regulated Gene 1 Protein