title: Exonuclease I aided enzyme-linked aptamer assay for small-molecule detection.
abstract: A novel enzyme-linked aptamer assay (ELAA) with the aid of Exonuclease I (Exo I) for colorimetric detection of small molecules was developed. The fluorescein isothiocyanate (FITC)-labeled aptamer was integrated into a double-stranded DNA (dsDNA). In the presence of target, the binding of aptamer with target protected the aptamer from Exo I degradation, which resulted in the FITC tag remaining on the aptamer. Then, the anti-FITC-HRP conjugate was used to produce an optically observable signal. By monitoring the color change, we were able to detect two model molecules, ATP and L-argininamide, with high selectivity and high sensitivity even in the serum matrix. It is expected to be a simple and general ELAA method with wide applicability.
authors.affiliation: Beijing National Laboratory for Molecular Science, Key Laboratory of Molecular Nanostructures and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Qiuling
authors.initials: Q
authors.lastname: Zhao
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.forename: Ted
authors.initials: T
authors.lastname: Xia
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.forename: Jianli
authors.initials: J
authors.lastname: Liu
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Fang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D003124:Colorimetry / Q000379:methods*; D057075:Enzyme Assays / Q000379:methods*; D005090:Exodeoxyribonucleases / Q000737:chemistry*; D052156:SELEX Aptamer Technique / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D052157:Aptamers, Nucleotide; D005090:Exodeoxyribonucleases; C028450:exodeoxyribonuclease I