title: Intravaginal administration of lactic acid bacteria modulated the incidence of purulent vaginal discharges, plasma haptoglobin concentrations, and milk production in dairy cows.
abstract: This investigation studied the effects of intravaginal administration of a mixture of lactic acid bacteria (LAB) on the incidence of purulent vaginal discharges (PVD), plasma haptoglobin concentrations, and milk production in dairy cows. A total of 82 pregnant primiparous and multiparous Holstein dairy cows were used in this study. Half of the cows received intravaginally 1mL of LAB at 10(10)-10(12)cfu/mL and the other half 1mL of reconstituted skim milk (i.e., carrier) (controls). Administration of LAB was conducted once per wk during 2 and 1wk before the expected day of calving and at 1, 2, 3, and 4wk postpartum. Data demonstrated that intravaginal administration of LAB decreased the occurrence of PVD at 3wk postpartum (P<0.05). Concentrations of plasma haptoglobin, an acute phase protein often associated with uterine infections, was lower in cows treated with the LAB mixture at 2wk (P<0.001) and 3wk (P<0.05) postpartum. Treatment with LAB did not improve overall pregnancy rate, but the treated multiparous cows produced more milk than their control counterparts (P<0.05), whereas no difference was observed in primiparous cows regarding milk yield (P>0.05). Overall, this is the first study demonstrating that intravaginal LAB administration lowers the incidence of PVD and enhances milk production in dairy cows. Further research is warranted to evaluate the effects of LAB on reproductive performance in a larger cohort of cows.
authors.affiliation: Department of Agricultural, Food, and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada. Electronic address: burim.ametaj@ualberta.ca.
authors.forename: B N
authors.initials: BN
authors.lastname: Ametaj
authors.affiliation: Department of Agricultural, Food, and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Iqbal
authors.affiliation: Department of Agricultural, Food, and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
authors.forename: F
authors.initials: F
authors.lastname: Selami
authors.affiliation: Department of Agricultural, Food, and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
authors.forename: J F
authors.initials: JF
authors.lastname: Odhiambo
authors.affiliation: Department of Agricultural, Food, and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Agricultural, Food, and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
authors.forename: M G
authors.initials: MG
authors.lastname: Gänzle
authors.affiliation: Department of Agricultural, Food, and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
authors.forename: S M
authors.initials: SM
authors.lastname: Dunn
authors.affiliation: Department of Agricultural, Food, and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada; Department for Farm Animals and Veterinary Public Health, Institute of Animal Nutrition and Functional Plant Compounds, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, Austria.
authors.forename: Q
authors.initials: Q
authors.lastname: Zebeli
mesh_terms: D000282:Administration, Intravaginal; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology* / Q000628:therapy; D005260:Female; D006242:Haptoglobins / Q000032:analysis; D007774:Lactation; D007778:Lactobacillus; D008892:Milk / Q000378:metabolism; D011247:Pregnancy; D011897:Random Allocation; D012044:Regression Analysis; D019522:Vaginal Discharge / Q000382:microbiology* / Q000628:therapy
chemical_list: D006242:Haptoglobins
keywords: Dairy cow; Haptoglobin; Lactic acid bacteria; Metritis; Reproduction