title: Neoglucosylated collagen matrices drive neuronal cells to differentiate.
abstract: Despite the relevance of carbohydrates as cues in eliciting specific biological responses, glycans have been rarely exploited in the study of neuronal physiology. We report thereby the study of the effect of neoglucosylated collagen matrices on neuroblastoma F11 cell line behavior. Morphological and functional analysis clearly showed that neoglucosylated collagen matrices were able to drive cells to differentiate. These data show for the first time that F11 cells can be driven from proliferation to differentiation without the use of chemical differentiating agents. Our work may offer to cell biologists new opportunities to study neuronal cell differentiation mechanisms in a cell environment closer to physiological conditions.
authors.affiliation: Department of Biotechnology and Biosciences, University of Milano-Bicocca , P.zza della Scienza 2, I-20126 Milano, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Russo
authors.forename: Antonella
authors.initials: A
authors.lastname: Sgambato
authors.forename: Marzia
authors.initials: M
authors.lastname: Lecchi
authors.forename: Valentina
authors.initials: V
authors.lastname: Pastori
authors.forename: Mario
authors.initials: M
authors.lastname: Raspanti
authors.forename: Antonino
authors.initials: A
authors.lastname: Natalello
authors.forename: Silvia M
authors.initials: SM
authors.lastname: Doglia
authors.forename: Francesco
authors.initials: F
authors.lastname: Nicotra
authors.forename: Laura
authors.initials: L
authors.lastname: Cipolla
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D003094:Collagen / Q000737:chemistry / Q000493:pharmacokinetics*; D005109:Extracellular Matrix / Q000378:metabolism*; D051379:Mice; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology*; D009474:Neurons / Q000378:metabolism* / Q000473:pathology*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: C022814:glycosylated collagen; D003094:Collagen