title: Glucuronidation of aurantio-obtusin: identification of human UDP-glucuronosyltransferases and species differences.
abstract: 1. The aurantio-obtusin's glucuronide was detected when aurantio-obtusin was incubated with human liver microsomes (HLMs). Recombinant UGT isoforms screening experiment showed that UGT1A8 was the major isoform contributed to the glucuronidation. 2. The metabolic profiles for aurantio-obtusin in liver microsomes from different species were similar, however, the intrinsic clearance values (Vmax/Km) among the species were: Monkey > Human > Rat > Rabbit > Dog > Pig > Mouse > Guinea pig.
authors.affiliation: Pharmaceutical College, Liaoning University of Traditional Chinese Medicine , Dalian , China .
authors.forename: Bao-Li
authors.initials: BL
authors.lastname: Mi
authors.forename: Qi
authors.initials: Q
authors.lastname: Sun
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Qu
authors.forename: Xiao-Xu
authors.initials: XX
authors.lastname: Gao
authors.forename: Zhen-Wen
authors.initials: ZW
authors.lastname: Yu
authors.forename: Guang-Bo
authors.initials: GB
authors.lastname: Ge
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Cai
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Yan-Chao
authors.initials: YC
authors.lastname: Zheng
authors.forename: Zhen-Qiu
authors.initials: ZQ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000880:Anthraquinones / Q000378:metabolism*; D057075:Enzyme Assays; D020719:Glucuronides / Q000737:chemistry / Q000378:metabolism*; D014453:Glucuronosyltransferase / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D013058:Mass Spectrometry; D008862:Microsomes, Liver / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D013045:Species Specificity
chemical_list: D000880:Anthraquinones; D020719:Glucuronides; D011994:Recombinant Proteins; C000595611:aurantio-obtusin; D014453:Glucuronosyltransferase
keywords: Aurantio-obtusin; UDP-glucuronosyltransferase (UGT); species difference