title: GnRH agonist vs. hCG for triggering of ovulation--differential effects on gene expression in human granulosa cells.
abstract: OBJECTIVE
  To investigate the mRNA expression of genes related to steroidogenesis and OHSS in granulosa cells (GCs) of patients triggered with GnRH agonist compared to patients triggered with hCG.
  
  
  METHODS
  Mural GCs were obtained at the time of oocyte retrieval and gene expression was analyzed using quantitative real time RT-PCR.
  
  
  METHODS
  Single center, case control study.
  
  
  METHODS
  24 women who were treated with GnRH agonist or hCG for triggering of ovulation.
  
  
  METHODS
  GC collection.
  
  
  METHODS
  The expression of genes related to steroidogenesis and OHSS in mural GCs.
  
  
  RESULTS
  The fertilization rate was similar in the two groups. The mRNA expression of CYP19A1 (0.50 vs 1, arbitrary unit), CYP11A1 (0.6 vs. 1) and 3 beta hydroxysteroid-dehydrogenase (0.39 vs 1) was significantly lower in the GnRH group. The expression of VEGF (0.74 vs. 1) and inhibin β B (0.38 vs 1) was lower in the GnRH analog triggered group.
  
  
  CONCLUSIONS
  Expression of genes related to steroidogenesis is lower at the time of oocyte retrieval in patients triggered with GnRH agonist. The decreased expression of VEGF and inhibin β B in the GnRH agonist group can explain the mechanism of early OHSS prevention.
authors.affiliation: Human Reproduction Lab and IVF Unit, Department of Obstetrics and Gynecology, Chaim Sheba Medical Center, Tel-Hashomer, Ramat Gan, Israel.
authors.forename: Jigal
authors.initials: J
authors.lastname: Haas
authors.affiliation: Human Reproduction Lab and IVF Unit, Department of Obstetrics and Gynecology, Chaim Sheba Medical Center, Tel-Hashomer, Ramat Gan, Israel.
authors.forename: Libby
authors.initials: L
authors.lastname: Ophir
authors.affiliation: Human Reproduction Lab and IVF Unit, Department of Obstetrics and Gynecology, Chaim Sheba Medical Center, Tel-Hashomer, Ramat Gan, Israel.
authors.forename: Eran
authors.initials: E
authors.lastname: Barzilay
authors.affiliation: Human Reproduction Lab and IVF Unit, Department of Obstetrics and Gynecology, Chaim Sheba Medical Center, Tel-Hashomer, Ramat Gan, Israel.
authors.forename: Gil M
authors.initials: GM
authors.lastname: Yerushalmi
authors.affiliation: Human Reproduction Lab and IVF Unit, Department of Obstetrics and Gynecology, Chaim Sheba Medical Center, Tel-Hashomer, Ramat Gan, Israel.
authors.forename: Yuval
authors.initials: Y
authors.lastname: Yung
authors.affiliation: Human Reproduction Lab and IVF Unit, Department of Obstetrics and Gynecology, Chaim Sheba Medical Center, Tel-Hashomer, Ramat Gan, Israel.
authors.forename: Alon
authors.initials: A
authors.lastname: Kedem
authors.affiliation: Human Reproduction Lab and IVF Unit, Department of Obstetrics and Gynecology, Chaim Sheba Medical Center, Tel-Hashomer, Ramat Gan, Israel.
authors.forename: Ettie
authors.initials: E
authors.lastname: Maman
authors.affiliation: Human Reproduction Lab and IVF Unit, Department of Obstetrics and Gynecology, Chaim Sheba Medical Center, Tel-Hashomer, Ramat Gan, Israel.
authors.forename: Ariel
authors.initials: A
authors.lastname: Hourvitz
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics; D000328:Adult; D001141:Aromatase / Q000235:genetics; D016022:Case-Control Studies; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D015870:Gene Expression / Q000187:drug effects; D007987:Gonadotropin-Releasing Hormone / Q000819:agonists* / Q000378:metabolism; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007265:Inhibins / Q000235:genetics; D054315:Oocyte Retrieval / Q000379:methods; D016471:Ovarian Hyperstimulation Syndrome / Q000235:genetics / Q000378:metabolism; D010060:Ovulation / Q000187:drug effects*; D010062:Ovulation Induction / Q000379:methods; D010750:Phosphoproteins / Q000235:genetics; D011974:Receptors, LH / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017329:Triptorelin Pamoate / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: D006063:Chorionic Gonadotropin; D010750:Phosphoproteins; D011974:Receptors, LH; D042461:Vascular Endothelial Growth Factor A; D000098682:Steroidogenic Acute Regulatory Protein; D017329:Triptorelin Pamoate; D007987:Gonadotropin-Releasing Hormone; D007265:Inhibins; D015096:3-Hydroxysteroid Dehydrogenases; D001141:Aromatase; C561410:CYP19A1 protein, human; D002786:Cholesterol Side-Chain Cleavage Enzyme