title: Extracellular vesicles in luminal fluid of the ovine uterus.
abstract: Microvesicles and exosomes are nanoparticles released from cells and can contain small RNAs, mRNA and proteins that affect cells at distant sites. In sheep, endogenous beta retroviruses (enJSRVs) are expressed in the endometrial epithelia of the uterus and can be transferred to the conceptus trophectoderm. One potential mechanism of enJSRVs transfer from the uterus to the conceptus is via exosomes/microvesicles. Therefore, studies were conducted to evaluate exosomes in the uterine luminal fluid (ULF) of sheep. Exosomes/microvesicles (hereafter referred to as extracellular vesicles) were isolated from the ULF of day 14 cyclic and pregnant ewes using ExoQuick-TC. Transmission electron microscopy and nanoparticle tracking analysis found the isolates contained vesicles that ranged from 50 to 200 nm in diameter. The isolated extracellular vesicles were positive for two common markers of exosomes (CD63 and HSP70) by Western blot analysis. Proteins in the extracellular vesicles were determined by mass spectrometry and Western blot analysis. Extracellular vesicle RNA was analyzed for small RNAs by sequencing and enJSRVs RNA by RT-PCR. The ULF extracellular vesicles contained a large number of small RNAs and miRNAs including 81 conserved mature miRNAs. Cyclic and pregnant ULF extracellular vesicles contained enJSRVs env and gag RNAs that could be delivered to heterologous cells in vitro. These studies support the hypothesis that ULF extracellular vesicles can deliver enJSRVs RNA to the conceptus, which is important as enJSRVs regulate conceptus trophectoderm development. Importantly, these studies support the idea that extracellular vesicles containing select miRNAs, RNAs and proteins are present in the ULF and likely have a biological role in conceptus-endometrial interactions important for the establishment and maintenance of pregnancy.
authors.affiliation: Department of Animal Sciences, Washington State University, Pullman, Washington, United States of America.
authors.forename: Gregory
authors.initials: G
authors.lastname: Burns
authors.affiliation: Department of Animal Sciences, Washington State University, Pullman, Washington, United States of America.
authors.forename: Kelsey
authors.initials: K
authors.lastname: Brooks
authors.affiliation: School of Molecular Biosciences, Washington State University, Pullman, Washington, United States of America.
authors.forename: Mark
authors.initials: M
authors.lastname: Wildung
authors.affiliation: Department of Molecular and Integrative Physiology, University of Kansas Medical School, Kansas City, Kansas, United States of America.
authors.forename: Raphatphorn
authors.initials: R
authors.lastname: Navakanitworakul
authors.affiliation: Department of Molecular and Integrative Physiology, University of Kansas Medical School, Kansas City, Kansas, United States of America.
authors.forename: Lane K
authors.initials: LK
authors.lastname: Christenson
authors.affiliation: Department of Animal Sciences, Washington State University, Pullman, Washington, United States of America.
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Spencer
mesh_terms: D000818:Animals; D015153:Blotting, Western; D022162:Cytoplasmic Vesicles / Q000378:metabolism* / Q000648:ultrastructure; D020077:Endogenous Retroviruses / Q000235:genetics; D045604:Extracellular Fluid / Q000378:metabolism*; D005260:Female; D057809:HEK293 Cells; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D053758:Nanoparticles / Q000648:ultrastructure; D011247:Pregnancy; D040901:Proteomics; D012367:RNA, Viral / Q000378:metabolism; D012756:Sheep; D060149:Tetraspanin 30 / Q000378:metabolism; D014599:Uterus / Q000378:metabolism*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D035683:MicroRNAs; D012367:RNA, Viral; D060149:Tetraspanin 30