title: Structural characterization and antioxidant activity of a heteropolysaccharide isolated from Hedysarum polybotrys.
abstract: A water-soluble polysaccharide (HPS3aS) with a molecular mass of 1.22 × 10(4) Da was isolated from Hedysarum polybotrys using anion-exchange and gel-permeation chromatography. HPS3aS exhibits a globular-shaped conformation in 0.1 M NaNO3 by size exclusion chromatography with multi-angle laser light scattering (SEC-MALLS). The investigation of the structural features of this heteropolysaccharide through a combination of chemical and instrumental analyses revealed that the backbone of HPS3aS is composed of α-D-(1 → 4)-linked glucopyranose residues, which occasionally branched at O-6. The branches are composed of (1 → 4)-linked galactopyranose residues and terminated with glucopyranose residues. HPS3aS possesses good in vitro antioxidant activity, as evaluated by scavenging assays with 1,1-diphenyl-2-picrylhydrazyl, hydroxyl, and superoxide radicals, which suggests that HPS3aS could be a potential antioxidant.
authors.affiliation: a The Second Clinical Medical College, Lanzhou University , Lanzhou 730030 , China.
authors.forename: Liang-Gong
authors.initials: LG
authors.lastname: Zhao
authors.forename: Tong-Qiang
authors.initials: TQ
authors.lastname: Chen
authors.forename: De-Mei
authors.initials: DM
authors.lastname: Feng
authors.forename: Ti-Gan
authors.initials: TG
authors.lastname: Xiao
authors.forename: Zi-Long
authors.initials: ZL
authors.lastname: Dang
authors.forename: Shi-Lan
authors.initials: SL
authors.lastname: Feng
authors.forename: Ya-Yi
authors.initials: YY
authors.lastname: Xia
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D007887:Fabaceae / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010084:Oxidation-Reduction; D010851:Picrates / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013481:Superoxides / Q000032:analysis / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; C000591878:HPS3aS polysaccharide; D010851:Picrates; D011134:Polysaccharides; D014867:Water; D013481:Superoxides; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Hedysarum polybotrys; antioxidant activity; polysaccharide; structure feature