title: Antimicrobial and antibiofilm effects of selected food preservatives against Salmonella spp. isolated from chicken samples.
abstract: Salmonella spp. are widespread foodborne pathogens that contaminate egg and poultry meats. Attachment, colonization, as well as biofilm formation capacity of Salmonella spp. on food and contact surfaces of food may cause continuous contamination. Biofilm may play a crucial role in the survival of salmonellae under unfavorable environmental conditions, such as in animal slaughterhouses and processing plants. This could serve as a reservoir compromising food safety and human health. Addition of antimicrobial preservatives extends shelf lives of food products, but even when products are supplemented with adequate amounts of preservatives, it is not always possible to inhibit the microorganisms in a biofilm community. In this study, our aims were i) to determine the minimum inhibitory concentrations (MIC) and minimum biofilm inhibitory concentrations (MBIC) of selected preservatives against planktonic and biofilm forms of Salmonella spp. isolated from chicken samples and Salmonella Typhimurium SL1344 standard strain, ii) to show the differences in the susceptibility patterns of same strains versus the planktonic and biofilm forms to the same preservative agent, and iii) to determine and compare antimicrobial and antibiofilm effects of selected food preservatives against Salmonella spp. For this purpose, Salmonella Typhimurium SL1344 standard strain and 4 Salmonella spp. strains isolated from chicken samples were used. Investigation of antimicrobial and antibiofilm effects of selected food preservatives against Salmonella spp. was done according to Clinical and Laboratory Standards Institute M100-S18 guidelines and BioTimer assay, respectively. As preservative agents, pure ciprofloxacin, sodium nitrite, potassium sorbate, sodium benzoate, methyl paraben, and propyl paraben were selected. As a result, it was determined that MBIC values are greater than the MIC values of the preservatives. This result verified the resistance seen in a biofilm community to food preservatives and highlighted this subject, not to be ignored in food applications.
authors.affiliation: Department of Food Analysis, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey.
authors.forename: Buket
authors.initials: B
authors.lastname: Er
authors.forename: Burak
authors.initials: B
authors.lastname: Demirhan
authors.forename: Fatma Kaynak
authors.initials: FK
authors.lastname: Onurdag
authors.forename: Selda Özgen
authors.initials: SÖ
authors.lastname: Ozgacar
authors.forename: Aysel Bayhan
authors.initials: AB
authors.lastname: Oktem
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D002645:Chickens; D005520:Food Preservatives / Q000494:pharmacology*; D008460:Meat / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D012475:Salmonella / Q000187:drug effects* / Q000502:physiology*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D005520:Food Preservatives
keywords: Salmonellaspp.; antibiofilm; antimicrobial; biofilm; food preservative