title: [Identification of microorganisms based on the effect of giant Raman scattering].
abstract: OBJECTIVE
  Development and evaluation of express diagnostics of microorganisms for reduction of time spent on study execution.
  
  
  METHODS
  Giant Raman scattering effect and proposed substrate with metallic nano-balls of silver were used for identification and indication of microorganisms. Staphylococcus aureus, Staphylococcus haemolyticus, Staphylococcus saprophyticus, Candida albicans, Escherichia coli, Bacillus subtillis, Pseudomonas aeruginosa were used as objects of study. All the microorganisms were cultivated in dense nutrient media and non-fluorescent microbiological membrane filters.
  
  
  RESULTS
  Giant Raman scattering method on the substrates used increases sensitivity of indication and specificity of identification of microbes compared with other existing express methods and allows to see the signal from a single bacterium when exposed to laser light. The methodic proposed was also used to determine sensitivity of bacteria to antimicrobial preparations.
  
  
  CONCLUSIONS
  The proposed hardware and method may be used for express diagnostics of processes of microbial nature.
authors.forename: M T
authors.initials: MT
authors.lastname: Aleksandrov
authors.forename: V I
authors.initials: VI
authors.lastname: Kukushkin
authors.forename: O A
authors.initials: OA
authors.lastname: Ambartsumian
authors.forename: E P
authors.initials: EP
authors.lastname: Pashkov
authors.forename: E V
authors.initials: EV
authors.lastname: Budanova
authors.forename: V M
authors.initials: VM
authors.lastname: Zuev
authors.forename: A S
authors.initials: AS
authors.lastname: Kalinichuk
authors.forename: T A
authors.initials: TA
authors.lastname: Khomeriki
authors.forename: A A
authors.initials: AA
authors.lastname: Labazanov
mesh_terms: D001419:Bacteria / Q000737:chemistry*; D002176:Candida albicans / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*