title: Down-regulation of miRNA-204 by LMP-1 enhances CDC42 activity and facilitates invasion of EBV-associated nasopharyngeal carcinoma cells.
abstract: Nasopharayngeal carcinoma (NPC) is an Epstein-Barr virus (EBV)-associated malignancy. It is known that microRNAs are implicated in the progression of NPC. However, the role of miR-204 in NPC is poorly understood. In this study, we found that miR-204 was down-regulated in NPC cells and tissues. Low-level expression of miR-204 was significantly associated with a more aggressive and poor prognostic phenotype of NPC. We further found that EBV-encoded latent membrane protein 1 (LMP-1) suppressed miR-204 expression by activating Stat-3. Cdc42 was identified as a direct target of miR-204. Mir-204 inhibited EBV positive C666-1 cell invasion and metastasis partly through targeting cdc42.
authors.affiliation: Affiliated Cancer Hospital of Guangzhou Medical University, Cancer Center of Guangzhou Medical University (CCGMU), Guangzhou, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Ma
authors.affiliation: Affiliated Cancer Hospital of Guangzhou Medical University, Cancer Center of Guangzhou Medical University (CCGMU), Guangzhou, People's Republic of China.
authors.forename: Xubin
authors.initials: X
authors.lastname: Deng
authors.affiliation: Department of Histology and Embryology, Guangdong Medical College, Zhanjiang, People's Republic of China.
authors.forename: Minhua
authors.initials: M
authors.lastname: Wu
authors.affiliation: Department of Radiotherapy, People's Hospital of Shanxi Province, Taiyuan, People's Republic of China.
authors.forename: Gong
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Affiliated Cancer Hospital of Guangzhou Medical University, Cancer Center of Guangzhou Medical University (CCGMU), Guangzhou, People's Republic of China. Electronic address: JianqingHuang_01@126.com.
authors.forename: Jianqing
authors.initials: J
authors.lastname: Huang
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002277:Carcinoma; D045744:Cell Line, Tumor; D018450:Disease Progression; D015536:Down-Regulation; D020031:Epstein-Barr Virus Infections / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D004854:Herpesvirus 4, Human; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology / Q000821:virology; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D011379:Prognosis; D034622:RNA Interference; D050796:STAT3 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction; D047368:Tumor Burden; D014763:Viral Matrix Proteins / Q000502:physiology*; D020764:cdc42 GTP-Binding Protein / Q000378:metabolism*
chemical_list: C037662:EBV-associated membrane antigen, Epstein-Barr virus; C549404:MIRN204 microRNA, human; D035683:MicroRNAs; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D014763:Viral Matrix Proteins; D020764:cdc42 GTP-Binding Protein
keywords: Cdc42; Epstein–Barr virus; Latent membrane protein 1; Nasopharyngeal carcinoma; Stat-3; miR-204