title: Peptidomic analysis of antimicrobial peptides in skin secretions of Amolops mantzorum.
abstract: Amphibian skin secretions contain abundant bioactive peptides that are valuable natural resources for human beings. However, many amphibians are disappearing from the world, making relevant scientific studies even more important. In this study, 24 cDNA sequences encoding antimicrobial peptide (AMP) precursors were initially cloned by screening a cDNA library derived from the skin of the Sichuan torrent frog, Amolops mantzorum. Eighteen mature AMPs belonging to 11 different families were deduced from these cDNA clones. Biological function was confirmed in each family of these AMPs. Some of them were purified from the skin secretions, and their molecular structures were determined by Edman degradation. Liquid chromatography in conjunction with tandem mass spectrometry (LC-MS/MS)-based peptidomics was used to further confirm the actual presence and characteristics of mature AMPs in the skin secretions of A. mantzorum. Incomplete tryptic digestion and gas-phase fractionation (GPF) analysis were used to increase the peptidome coverage and reproducibility of peptide ion selection.
authors.affiliation: 1 Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang, Hebei 050024, China.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Hu
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Yu
authors.forename: Shiqi
authors.initials: S
authors.lastname: Xu
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Hu
authors.forename: Chao
authors.initials: C
authors.lastname: Guo
authors.forename: Fengjiao
authors.initials: F
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Jingze
authors.initials: J
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D002853:Chromatography, Liquid; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004912:Erythrocytes / Q000187:drug effects / Q000648:ultrastructure; D004926:Escherichia coli / Q000187:drug effects; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D011898:Ranidae / Q000502:physiology*; D012867:Skin / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D023181:Antimicrobial Cationic Peptides; D018076:DNA, Complementary