title: Biological imaging with coherent Raman scattering microscopy: a tutorial.
abstract: Coherent Raman scattering (CRS) microscopy is gaining acceptance as a valuable addition to the imaging toolset of biological researchers. Optimal use of this label-free imaging technique benefits from a basic understanding of the physical principles and technical merits of the CRS microscope. This tutorial offers qualitative explanations of the principles behind CRS microscopy and provides information about the applicability of this nonlinear optical imaging approach for biological research.
authors.affiliation: University of California, Beckman Laser Institute, Irvine, California 92697.
authors.forename: Alba
authors.initials: A
authors.lastname: Alfonso-García
authors.affiliation: University of California, Beckman Laser Institute, Irvine, California 92697.
authors.forename: Richa
authors.initials: R
authors.lastname: Mittal
authors.affiliation: Center for Nano-Bio Technology, Division of Convergence Technology, Korea Research Institute of Standards and Science, 1 Doryong-Dong, Yuseong-Gu, Daejeon 305-340, Republic of Korea.
authors.forename: Eun Seong
authors.initials: ES
authors.lastname: Lee
authors.affiliation: University of California, Beckman Laser Institute, Irvine, California 92697.
authors.forename: Eric O
authors.initials: EO
authors.lastname: Potma
mesh_terms: D000818:Animals; D004867:Equipment Design; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007834:Lasers; D008027:Light; D008537:Meibomian Glands / Q000473:pathology; D051379:Mice; D008853:Microscopy / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods; D055095:Optics and Photonics; D017785:Photons; D012542:Scattering, Radiation; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014732:Vibration