title: Proof-of-concept for a virus-induced obesity vaccine; vaccination against the obesity agent adenovirus 36.
abstract: Human adenovirus 36 (Ad36) is positively associated with obesity in humans and animals. Ad36 infection is characterized by increased adiposity and inflammation. To investigate the possibility that a prophylactic vaccine candidate might protect against Ad36-induced obesity and inflammation, we purified Ad36 and ultraviolet-irradiated virus to obtain a vaccine candidate. After immunizing the mice with the vaccine candidate (vaccinated group), live Ad36 was injected into mice as a challenge test. Unvaccinated mice (control group) were immunized with phosphate-buffered saline and then challenged with live Ad36. Fourteen weeks after challenge, we compared adiposity and inflammation in vaccinated and control mice. The control group showed 17% greater body weight and 20% more epididymal fats compared with the vaccinated group. In addition, the vaccinated group had decreased serum levels of pro-inflammatory cytokines, and infiltrated immune cells, especially M1 macrophages, in fat tissue. Therefore, the vaccine candidate for Ad36 was able to protect against Ad36-increased body weight and fat as well as inflammatory states after challenge. These results provide proof-of-concept for prophylactic vaccination against virus-induced adiposity.
authors.affiliation: Department of Biotechnology, The Catholic University of Korea, Bucheon, Korea.
authors.forename: H-N
authors.initials: HN
authors.lastname: Na
authors.affiliation: Department of Biotechnology, The Catholic University of Korea, Bucheon, Korea.
authors.forename: J-H
authors.initials: JH
authors.lastname: Nam
mesh_terms: D000256:Adenoviridae / Q000472:pathogenicity*; D000257:Adenoviridae Infections / Q000150:complications / Q000276:immunology*; D017667:Adipocytes / Q000378:metabolism / Q000821:virology*; D000818:Animals; D007249:Inflammation / Q000517:prevention & control / Q000821:virology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000517:prevention & control / Q000821:virology*; D014765:Viral Vaccines; D015430:Weight Gain / Q000187:drug effects
chemical_list: D014765:Viral Vaccines