title: Rapid saccharification for production of cellulosic biofuels.
abstract: The economical production of biofuels is hindered by the recalcitrance of lignocellulose to processing, causing high consumption of processing enzymes and impeding hydrolysis of pretreated lignocellulosic biomass. We determined the major rate-limiting factor in the hydrolysis of popping pre-treated rice straw (PPRS) by examining cellulase adsorption to lignin and cellulose, amorphogenesis of PPRS, and re-hydrolysis. Based on the results, equivalence between enzyme loading and the open structural area of cellulose was required to significantly increase productive adsorption of cellulase and to accelerate enzymatic saccharification of PPRS. Amorphogenesis of PPRS by phosphoric acid treatment to expand open structural area of the cellulose fibers resulted in twofold higher cellulase adsorption and increased the yield of the first re-hydrolysis step from 13% to 46%. The total yield from PPRS was increased to 84% after 3h. These results provide evidence that cellulose structure is one of major effects on the enzymatic hydrolysis.
authors.affiliation: Bio-energy Research Institute, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Dae-Seok
authors.initials: DS
authors.lastname: Lee
authors.affiliation: Bio-energy Research Institute, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Seung Gon
authors.initials: SG
authors.lastname: Wi
authors.affiliation: Bio-energy Research Institute, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Soo Jung
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Department of Forest Products and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Yoon-Gyo
authors.initials: YG
authors.lastname: Lee
authors.affiliation: Department of Forest Products, Kookmin University, Seoul 136-702, Republic of Korea.
authors.forename: Yeong-Suk
authors.initials: YS
authors.lastname: Kim
authors.affiliation: Bio-energy Research Institute, Chonnam National University, Gwangju 500-757, Republic of Korea; Department of Forest Products and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea; Department of Bioenergy Science and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea. Electronic address: baehj@chonnam.ac.kr.
authors.forename: Hyeun-Jong
authors.initials: HJ
authors.lastname: Bae
mesh_terms: D000327:Adsorption; D056804:Biofuels; D019149:Bioreactors; D050260:Carbohydrate Metabolism; D002482:Cellulose / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis
chemical_list: D056804:Biofuels; D002482:Cellulose
keywords: Amorphogenesis; Biofuels; Enzymatic hydrolysis; Enzyme adsorption; Lignocellulose