title: Comparison of JEV neutralization assay using pseudotyped JEV with the conventional plaque-reduction neutralization test.
abstract: We previously reported the development of a neutralization assay system for evaluating Japanese Encephalitis Virus (JEV) neutralizing antibody (NAb) using pseudotyped-JEV (JEV-PV). JEV-PV-based neutralization assay offers several advantages compared with the current standard plaque-reduction neutralization test (PRNT), including simplicity, safety, and speed. To evaluate the suitability of the JEV-PV assay as new replacement neutralization assay, we compared its repeatability, reproducibility, specificity, and correlated its results with those obtained using the PRNT. These analyses showed a close correlation between the results obtained with the JEV-PV assay and the PRNT, using the 50% plaque reduction method as a standard for measuring NAb titers to JEV. The validation results met all analytical acceptance criteria. These results suggest that the JEV-PV assay could serve as a safe and simple method for measuring NAb titer against JEV and could be used as an alternative approach for assaying the potency of JEV neutralization.
authors.affiliation: Department of Bio-industrial technologies, Konkuk University, Seoul, 143-701, Republic of Korea.
authors.forename: Hee-Jung
authors.initials: HJ
authors.lastname: Lee
authors.forename: Kyung-Il
authors.initials: KI
authors.lastname: Min
authors.forename: Ki Hoon
authors.initials: KH
authors.lastname: Park
authors.forename: Hyo Jung
authors.initials: HJ
authors.lastname: Choi
authors.forename: Min-Kyoung
authors.initials: MK
authors.lastname: Kim
authors.forename: Chi-Young
authors.initials: CY
authors.lastname: Ahn
authors.forename: Young-Jin
authors.initials: YJ
authors.lastname: Hong
authors.forename: Young Bong
authors.initials: YB
authors.lastname: Kim
mesh_terms: D057134:Antibodies, Neutralizing / Q000032:analysis*; D000956:Antigens, Viral; D018349:Encephalitis Viruses, Japanese / Q000235:genetics / Q000276:immunology*; D009500:Neutralization Tests / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D010948:Viral Plaque Assay / Q000379:methods*
chemical_list: D057134:Antibodies, Neutralizing; D000956:Antigens, Viral