title: Overexpression of a novel MADS-box gene SlFYFL delays senescence, fruit ripening and abscission in tomato.
abstract: MADS-domain proteins are important transcription factors involved in many biological processes of plants. In our study, a tomato MADS-box gene, SlFYFL, was isolated. SlFYFL is expressed in all tissues of tomato and significantly higher in mature leave, fruit of different stages, AZ (abscission zone) and sepal. Delayed leaf senescence and fruit ripening, increased storability and longer sepals were observed in 35S:FYFL tomato. The accumulation of carotenoid was reduced, and ethylene content, ethylene biosynthetic and responsive genes were down-regulated in 35S:FYFL fruits. Abscission zone (AZ) did not form normally and abscission zone development related genes were declined in AZs of 35S:FYFL plants. Yeast two-hybrid assay revealed that SlFYFL protein could interact with SlMADS-RIN, SlMADS1 and SlJOINTLESS, respectively. These results suggest that overexpression of SlFYFL regulate fruit ripening and development of AZ via interactions with the ripening and abscission zone-related MADS box proteins.
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Campus A, 174 Shapingba Main Street, Chongqing 400044, People's Republic of China.
authors.forename: Qiaoli
authors.initials: Q
authors.lastname: Xie
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Campus A, 174 Shapingba Main Street, Chongqing 400044, People's Republic of China.
authors.forename: Zongli
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Campus A, 174 Shapingba Main Street, Chongqing 400044, People's Republic of China.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Campus A, 174 Shapingba Main Street, Chongqing 400044, People's Republic of China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Dong
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Campus A, 174 Shapingba Main Street, Chongqing 400044, People's Republic of China.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Campus A, 174 Shapingba Main Street, Chongqing 400044, People's Republic of China.
authors.forename: Baolu
authors.initials: B
authors.lastname: Cui
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Campus A, 174 Shapingba Main Street, Chongqing 400044, People's Republic of China.
authors.forename: Guoping
authors.initials: G
authors.lastname: Chen
mesh_terms: D000375:Aging / Q000235:genetics*; D000595:Amino Acid Sequence; D002338:Carotenoids / Q000096:biosynthesis; D005030:Ethylenes / Q000096:biosynthesis; D035264:Flowers / Q000378:metabolism / Q000502:physiology*; D005638:Fruit / Q000378:metabolism / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D018551:Solanum lycopersicum / Q000235:genetics* / Q000378:metabolism; D026161:MADS Domain Proteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D005030:Ethylenes; D026161:MADS Domain Proteins; D010940:Plant Proteins; D002338:Carotenoids; C036216:ethylene