title: The Arabidopsis thaliana At4g13040 gene, a unique member of the AP2/EREBP family, is a positive regulator for salicylic acid accumulation and basal defense against bacterial pathogens.
abstract: The Arabidopsis genome contains a large number of putative transcription factors, containing a DNA binding domain similar to APETALA2/ethylene response element binding protein (AP2/EREBP), for most of which a function is not known. Phylogenetic analysis divides the Apetala 2 (AP2) super-family into 5 major groups: AP2, RAV, ethylene response factor (ERF), dehydration response element binding protein (DREB) and At4g13040. Similar to ERF and DREB, the At4g13040 protein contains only one AP2 domain; however, its structural uniqueness places it into a distinct group. In this article, we report that At4g13040 (referred herein as Apetala 2 family protein involved in SA mediated disease defense 1 - APD1) is an important regulator for SA mediated plant defense. The APD1 gene is upregulated upon pathogen inoculation, exogenous SA application and in the mutant that constitutively activates SA signaling. The T-DNA insertion lines (inserted in the APD1 promoter), which fail to induce expression upon pathogen inoculation, are compromised for resistance against virulent bacterial pathogens and show reduced induction of pathogenesis related 1 gene. Our results suggest that APD1 functions downstream of PAD4 in Arabidopsis and promotes pathogen-induced SA accumulation. Exogenous SA application completely restores the loss-of-resistance phenotype of the apd1 mutant. Thus, APD1 is a positive regulator of disease defense that functions upstream of SA accumulation.
authors.affiliation: Room #415, School of life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
authors.forename: Mrunmay Kumar
authors.initials: MK
authors.lastname: Giri
authors.affiliation: Room #415, School of life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
authors.forename: Swadhin
authors.initials: S
authors.lastname: Swain
authors.affiliation: Room #415, School of life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
authors.forename: Janesh Kumar
authors.initials: JK
authors.lastname: Gautam
authors.affiliation: Room #415, School of life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
authors.forename: Subaran
authors.initials: S
authors.lastname: Singh
authors.affiliation: Room #415, School of life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
authors.forename: Nidhi
authors.initials: N
authors.lastname: Singh
authors.affiliation: Room #415, School of life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
authors.forename: Lipika
authors.initials: L
authors.lastname: Bhattacharjee
authors.affiliation: Room #415, School of life Sciences, Jawaharlal Nehru University, New Delhi 110067, India. Electronic address: ashis_nandi@yahoo.com.
authors.forename: Ashis Kumar
authors.initials: AK
authors.lastname: Nandi
mesh_terms: D000085:Acetates / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D009154:Mutation; D054883:Oxylipins / Q000378:metabolism; D010641:Phenotype; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D010937:Plant Growth Regulators / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D044224:Pseudomonas syringae / Q000502:physiology; D020156:Salicylic Acid / Q000378:metabolism* / Q000494:pharmacology; D015398:Signal Transduction; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D000085:Acetates; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D004268:DNA-Binding Proteins; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D014157:Transcription Factors; C093680:ethylene-responsive element binding protein; C072239:methyl jasmonate; D020156:Salicylic Acid
keywords: AP2/EREBP; At4g13040; Pseudomonas syringae; SA biosynthesis; Salicylic acid