title: The ileum positively regulates hepatic regeneration in rats.
abstract: OBJECTIVE
  To test the hypothesis that liver regeneration after partial hepatectomy can be influenced by the ileum.
  
  
  METHODS
  Eighteen Wistar rats were distributed into groups of six animals: 1 - ileum resection+ hepatectomy 2/3; 2 - hepatectomy 2/3, and 3 - sham. Anesthesia with ketamine and xylazine i.p., aseptic technique, analgesia with meperidine (10mg/kg s.c.). On day 6, serum ALT, AST, alkaline phosphatase (AP) and albumin were measured. Liver regeneration and hepatocyte mitosis were quantified. Statistical analysis with ANOVA and Tukey tests, with significance p<0.05.
  
  
  RESULTS
  In group hepatectomy+ileal resection, ALT, AST and AP were 180.6±24.9, 58.6±3.1 and 254.6±46.6 respectively. They were significantly higher than in the hepatectomy group, whose values were 126.0±16.5, 44.1±3.9 and 163.5±8.6, respectively (p<0.001). Albumin levels were not significantly different among groups. Liver regeneration in hepatectomy group (94.17%) was statistically higher (p<0.001) than in ileal resection+hepatectomy group (55.96%). In the latter group the mitosis of hepatocytes were significantly less frequent than in the hepatectomy group.
  
  
  CONCLUSIONS
  The data confirm that the ileum positively influence on liver regeneration in rats undergoing hepatectomy.
authors.affiliation: Federal University of Rio Grande do Norte, Department of Surgery, NatalRN, Brazil, Full Professor, Chairman, Department of Surgery, Federal University of Rio Grande do Norte (UFRN), Natal-RN, Brazil. Conception, design, intellectual and scientific content of the study; analysis and interpretation of data; technical procedures; critical revision.
authors.forename: Aldo Cunha
authors.initials: AC
authors.lastname: Medeiros
authors.affiliation: UFRN, NatalRN, Brazil, Graduate student, Scientific Initiation Program, UFRN, Natal-RN, Brazil. Acquisition of data, manuscript preparation.
authors.forename: Ana Clara Batista
authors.initials: AC
authors.lastname: Azevedo
authors.affiliation: UFRN, NatalRN, Brazil, Graduate student, Scientific Initiation Program, UFRN, Natal-RN, Brazil. Acquisition of data, manuscript preparation.
authors.forename: Jéssica Mayara de Figueiredo
authors.initials: JM
authors.lastname: Oséas
authors.affiliation: UFRN, NatalRN, Brazil, Graduate student, Scientific Initiation Program, UFRN, Natal-RN, Brazil. Acquisition of data, manuscript preparation.
authors.forename: Mariana Davim Ferreira
authors.initials: MD
authors.lastname: Gomes
authors.affiliation: UFRN, NatalRN, Brazil, Graduate student, Scientific Initiation Program, UFRN, Natal-RN, Brazil. Acquisition of data, manuscript preparation.
authors.forename: Fernanda Gurgel de
authors.initials: FG
authors.lastname: Oliveira
authors.affiliation: UFRN, Department of Pathology, NatalRN, Brazil, Associate Professor, Department of Pathology, UFRN, Natal-RN, Brazil. Acquisition and interpretation of data.
authors.forename: Keyla Borges Ferreira
authors.initials: KB
authors.lastname: Rocha
authors.affiliation: UFRN, Department of Surgery, NatalRN, Brazil, Master, Statistician, Department of Surgery, UFRN, Natal-RN, Brazil. Design of the study, interpretation of data, statistical analysis.
authors.forename: Italo Medeiros
authors.initials: IM
authors.lastname: Azevedo
authors.affiliation: UFRN, NatalRN, Brazil, Fellow PhD degree, Postgraduate Program in Health Sciences, UFRN, Natal-RN, Brazil. Acquisition and interpretation of data.
authors.forename: Amália Cínthia Meneses
authors.initials: AC
authors.lastname: Rêgo
authors.affiliation: UFRN, NatalRN, Brazil, Fellow PhD degree, Postgraduate Program in Health Sciences, UFRN, Natal-RN, Brazil. Acquisition and interpretation of data.
authors.forename: Marília Daniela Ferreira
authors.initials: MD
authors.lastname: Carvalho
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000418:Albumins / Q000032:analysis; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D006498:Hepatectomy; D022781:Hepatocytes / Q000502:physiology; D007082:Ileum / Q000502:physiology* / Q000601:surgery; D008115:Liver Regeneration / Q000502:physiology*; D008938:Mitosis / Q000502:physiology; D023421:Models, Animal; D009929:Organ Size; D011184:Postoperative Period; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000418:Albumins; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase