title: Saccharomyces boulardii expresses neuraminidase activity selective for α2,3-linked sialic acid that decreases Helicobacter pylori adhesion to host cells.
abstract: Helicobacter pylori is a major causative agent of gastritis and peptic ulcer disease and is an established risk factor for gastric malignancy. Antibiotic combination therapy can eradicate H. pylori. As these same regimens can evoke adverse effects and resistance, new alternative therapies or adjunctive treatments are needed. A probiotic approach may provide a novel strategy for H. pylori treatment. In the current study, two probiotic bacteria, Lactobacillus acidophilus and Lactobacillus reuteri, and a probiotic yeast, Saccharomyces boulardii, were evaluated for their ability to influence H. pylori viability, adherence to gastric and duodenal cells, as well as the effect of S. boulardii on cell surface expression of sialic acid. Our results indicate that S. boulardii contains neuraminidase activity selective for α(2-3)-linked sialic acid. This neuraminidase activity removes surface α(2-3)-linked sialic acid, the ligand for the sialic acid-binding H. pylori adhesin, which in turn, inhibits H. pylori adherence to duodenal epithelial cells.
authors.affiliation: Department of Infectious Diseases and Clinical Microbiology, School of Medicine, Adnan Menderes University, Aydin.
authors.forename: Serhan
authors.initials: S
authors.lastname: Sakarya
authors.forename: Necati
authors.initials: N
authors.lastname: Gunay
mesh_terms: D018829:Adhesins, Bacterial / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D002460:Cell Line; D004386:Duodenum / Q000382:microbiology; D004847:Epithelial Cells / Q000378:metabolism; D016481:Helicobacter Infections / Q000378:metabolism; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D007779:Lactobacillus acidophilus / Q000378:metabolism; D052200:Limosilactobacillus reuteri / Q000378:metabolism; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D009439:Neuraminidase / Q000378:metabolism*; D019936:Probiotics / Q000378:metabolism; D012440:Saccharomyces / Q000378:metabolism*; D013270:Stomach / Q000382:microbiology
chemical_list: D018829:Adhesins, Bacterial; D000900:Anti-Bacterial Agents; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid
keywords: Helicobacter pylori; Saccharomyces boulardii; neuraminidase; sialic acid