title: Deammonification process start-up after enrichment of anammox microorganisms from reject water in a moving-bed biofilm reactor.
abstract: Deammonification via intermittent aeration in biofilm process for the treatment of sewage sludge digester supernatant (reject water) was started up using two opposite strategies. Two moving-bed biofilm reactors were operated for 2.5 years at 26 (+/- 0.5 degree C with spiked influent(and hence free ammonia (FA)) addition. In the first start-up strategy, an enrichment of anammox biomass was first established, followed by the development of nitrifying biomass in the system (R1). In contrast, the second strategy aimed at the enrichment of anammox organisms into a nitrifying biofilm (R2). The first strategy was most successful, reaching higher maximum total nitrogen (TN) removal rates over a shorter start-up period. For both reactors, increasing FA spiking frequency and increasing effluent concentrations of the anammox intermediate hydrazine correlated to decreasing aerobic nitrate production (nitritation). The bacterial consortium of aerobic and anaerobic ammonium oxidizing bacteria in the bioreactor was determined via denaturing gel gradient electrophoresis, polymerase chain reaction and pyrosequencing. In addition to a shorter start-up with a better TN removal rate, nitrite oxidizing bacteria (Nitrospira) were outcompeted by spiked ammonium feeding from R1.
authors.forename: Ivar
authors.initials: I
authors.lastname: Zekker
authors.forename: Ergo
authors.initials: E
authors.lastname: Rikmann
authors.forename: Toomas
authors.initials: T
authors.lastname: Tenno
authors.forename: Kristel
authors.initials: K
authors.lastname: Kroon
authors.forename: Priit
authors.initials: P
authors.lastname: Vabamäe
authors.forename: Erik
authors.initials: E
authors.lastname: Salo
authors.forename: Liis
authors.initials: L
authors.lastname: Loorits
authors.forename: Sergio S C dC
authors.initials: SS
authors.lastname: Rubin
authors.forename: Siegfried E
authors.initials: SE
authors.lastname: Vlaeminck
authors.forename: Taavo
authors.initials: T
authors.lastname: Tenno
mesh_terms: D064751:Ammonium Compounds; D001421:Bacteria, Anaerobic / Q000378:metabolism*; D018441:Biofilms; D019149:Bioreactors / Q000382:microbiology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007220:Industrial Waste / Q000032:analysis / Q000517:prevention & control*; D012722:Sewage / Q000382:microbiology*; D014865:Waste Disposal, Fluid / Q000295:instrumentation* / Q000379:methods; D014874:Water Pollutants, Chemical / Q000302:isolation & purification / Q000378:metabolism*; D018508:Water Purification / Q000295:instrumentation* / Q000379:methods
chemical_list: D064751:Ammonium Compounds; D007220:Industrial Waste; D012722:Sewage; D014874:Water Pollutants, Chemical