title: Efficient human breast cancer xenograft regression after a single treatment with a novel liposomal formulation of epirubicin prepared using the EDTA ion gradient method.
abstract: Liposomes act as efficient drug carriers. Recently, epirubicin (EPI) formulation was developed using a novel EDTA ion gradient method for drug encapsulation. This formulation displayed very good stability and drug retention in vitro in a two-year long-term stability experiment. The cryo-TEM images show drug precipitate structures different than ones formed with ammonium sulfate method, which is usually used to encapsulate anthracyclines. Its pharmacokinetic properties and its efficacy in the human breast MDA-MB-231 cancer xenograft model were also determined. The liposomal EPI formulation is eliminated slowly with an AUC of 7.6487, while the free drug has an AUC of only 0.0097. The formulation also had a much higher overall antitumor efficacy than the free drug.
authors.affiliation: Department of Lipids and Liposomes, Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Gubernator
authors.affiliation: Department of Lipids and Liposomes, Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland.
authors.forename: Dominik
authors.initials: D
authors.lastname: Lipka
authors.affiliation: Department of Lipids and Liposomes, Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland.
authors.forename: Mariola
authors.initials: M
authors.lastname: Korycińska
authors.affiliation: Department of Experimental Oncology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of Sciences, Wrocław, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kempińska
authors.affiliation: Department of Experimental Oncology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of Sciences, Wrocław, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Milczarek
authors.affiliation: Department of Experimental Oncology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of Sciences, Wrocław, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Wietrzyk
authors.affiliation: Department of Personal Protective Equipment. Central Institute for Labour Protection - National Research Institute, Warsaw, Poland.
authors.forename: Rafał
authors.initials: R
authors.lastname: Hrynyk
authors.affiliation: Department of Pharmaceutical Technology, Albert-Ludwigs University, Freiburg, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Barnert
authors.affiliation: Department of Pharmaceutical Technology, Albert-Ludwigs University, Freiburg, Germany.
authors.forename: Regine
authors.initials: R
authors.lastname: Süss
authors.affiliation: Department of Lipids and Liposomes, Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland.
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Kozubek
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry / Q000494:pharmacology; D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002784:Cholesterol / Q000737:chemistry; D004492:Edetic Acid / Q000737:chemistry*; D015251:Epirubicin / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D008081:Liposomes; D008297:Male; D051379:Mice; D010713:Phosphatidylcholines / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D008081:Liposomes; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; C038089:1,2-distearoylphosphatidylethanolamine; D011092:Polyethylene Glycols; D015251:Epirubicin; D002784:Cholesterol; D004492:Edetic Acid