title: Prevalence and characterisation of quinolone resistance mechanisms in Salmonella spp.
abstract: The study was focused on characterisation of quinolone resistance mechanisms in Salmonella isolated from animals, food, and feed between 2008 and 2011. Testing of Minimal Inhibitory Concentrations revealed 6.4% of 2680 isolates conferring ciprofloxacin resistance. Simultaneously 37.7% and 40.8% were accounted for, respectively, nalidixic acid and ciprofloxacin Non Wild-Type populations. Amplification and sequencing of quinolone resistance determining region of topoisomerases genes in 44 isolates identified multiple amino-acid substitutions in gyrA at positions Ser83 (N=22; → Leu, → Phe, → Tyr), Asp87 (N=22; → Asn, → Gly, → Tyr) and parC (Thr57Ser, N=23; Ala141Ser, N=1). No relevant mutations were identified in gyrB and parE. Twelve patterns combining one or two substitutions were related to neither serovar nor ciprofloxacin MIC. In 92 isolates suspected for plasmid mediated quinolone resistance two qnr alleles were found: qnrS1 (or qnrS3; N=50) and qnrB19 (or qnrB10; N=24). Additionally, two isolates with chromosomally encoded mechanisms carried qnrS1 and qnrS2. All tested isolates were negative for qnrA, qnrC, qnrD, qepA, aac(6')-Ib-cr. Both chromosomal and plasmid mediated quinolone resistance determinants were found in several Salmonella serovars and Pulsed Field Gel Electrophoresis was used to assess phylogenetic similarity of selected isolates (N=82). Salmonella Newport was found to accumulate quinolone resistance determinants and the serovar was spreading clonally with either variable gyrA mutations, qnrS1/S3, or qnrB10/B19. Alternatively, various determinants are dispersed among related S. Enteritidis isolates. Antimicrobial selection pressure, multiple resistance determinants and scenarios for their acquisition and spread make extremely difficult to combat quinolone resistance.
authors.affiliation: Department of Microbiology, National Reference Laboratory for Salmonellosis and Antimicrobial Resistance, National Veterinary Research Institute, Partyzantów 57, 24-100 Puławy, Poland. Electronic address: wasyl@piwet.pulawy.pl.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Wasyl
authors.affiliation: Department of Microbiology, National Reference Laboratory for Salmonellosis and Antimicrobial Resistance, National Veterinary Research Institute, Partyzantów 57, 24-100 Puławy, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Hoszowski
authors.affiliation: Department of Microbiology, National Reference Laboratory for Salmonellosis and Antimicrobial Resistance, National Veterinary Research Institute, Partyzantów 57, 24-100 Puławy, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Zając
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D000821:Animal Feed / Q000382:microbiology*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D002939:Ciprofloxacin / Q000494:pharmacology*; D027081:DNA Gyrase / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D008826:Microbial Sensitivity Tests; D009268:Nalidixic Acid / Q000494:pharmacology; D010802:Phylogeny; D011044:Poland / Q000453:epidemiology; D015995:Prevalence; D015363:Quinolones / Q000494:pharmacology; D012475:Salmonella / Q000187:drug effects / Q000235:genetics*; D012481:Salmonella Infections, Animal / Q000453:epidemiology* / Q000382:microbiology; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D015363:Quinolones; D009268:Nalidixic Acid; D002939:Ciprofloxacin; D027081:DNA Gyrase
keywords: Epidemiology; PMQR; QRDR; Qnr; Quinolone resistance; Salmonella Newport