title: KLF8 knockdown suppresses proliferation and invasion in human osteosarcoma cells.
abstract: Krüppel-like factor 8 (KLF8) is a transcription factor that is important in the regulation of the cell cycle and has a critical role in oncogenic transformation and epithelial to mesenchymal transition (EMT). EMT is a key process in tumor metastasis. Although overexpression of KLF8 has been observed in a variety of human tumor types, the role of KLF8 in human osteosarcoma is yet to be elucidated. The present study aimed to investigate the biological impact of KLF8 on Saos-2 osteosarcoma cells. KLF8 gene expression was knocked down in vitro using a lentivirus-mediated small interfering (si)RNA method. Cell proliferation and cell cycle distribution were evaluated using 3-(4,5)-dimethylthiahiazo(-z-yl)-3,5-di-phenytetrazoliumromide and colony formation assays, and flow cytometry, respectively. Cell invasion was analyzed using a Transwell® invasion assay. Knockdown of KLF8 was found to significantly inhibit proliferation and invasion in osteosarcoma cells. These data suggest that KLF8 may exhibit an important role in osteosarcoma tumorigenesis and that KLF8 may be a potential therapeutic target for the treatment of osteosarcoma.
authors.affiliation: Graduate School of Medicine, Soochow University, Suzhou, Jiangsu 215006, P.R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Lin
authors.affiliation: Department of Oncology, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China.
authors.forename: Zan
authors.initials: Z
authors.lastname: Shen
authors.affiliation: Department of Oncology, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China.
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Tang
authors.affiliation: Department of Oncology, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China.
authors.forename: Shui-Er
authors.initials: SE
authors.lastname: Zheng
authors.affiliation: Department of Oncology, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China.
authors.forename: Yuan-Jue
authors.initials: YJ
authors.lastname: Sun
authors.affiliation: Department of Oncology, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China.
authors.forename: Da-Liu
authors.initials: DL
authors.lastname: Min
authors.affiliation: Department of Oncology, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yao
mesh_terms: D059447:Cell Cycle Checkpoints / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D016193:G1 Phase; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D016086:Lentivirus / Q000235:genetics; D009361:Neoplasm Invasiveness; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology*; D034622:RNA Interference; D012097:Repressor Proteins / Q000235:genetics*; D016192:Resting Phase, Cell Cycle
chemical_list: C507402:KLF8 protein, human; D051741:Kruppel-Like Transcription Factors; D012097:Repressor Proteins