title: Histological analyses demonstrate the temporary contribution of yolk sac, liver, and bone marrow to hematopoiesis during chicken development.
abstract: The use of avian animal models has contributed to the understanding of many aspects of the ontogeny of the hematopoietic system in vertebrates. However, specific events that occur in the model itself are still unclear. There is a lack of consensus, among previous studies, about which is the intermediate site responsible for expansion and differentiation of hematopoietic cells, and the liver's contribution to the development of this system. Here we aimed to evaluate the presence of hematopoiesis in the yolk sac and liver in chickens, from the stages of intra-aortic clusters in the aorta-genital ridges-mesonephros (AGM) region until hatching, and how it relates to the establishment of the bone marrow. Gallus gallus domesticus L. embryos and their respective yolk sacs at embryonic day 3 (E3) and up to E21 were collected and processed according to standard histological techniques for paraffin embedding. The slides were stained with hematoxylin-eosin, Lennert's Giemsa, and Sirius Red at pH 10.2, and investigated by light microscopy. This study demonstrated that the yolk sac was a unique hematopoietic site between E4 and E12. Hematopoiesis occurred in the yolk sac and bone marrow between E13 and E20. The liver showed granulocytic differentiation in the connective tissue of portal spaces at E15 and onwards. The yolk sac showed expansion of erythrocytic and granulocytic lineages from E6 to E19, and E7 to E20, respectively. The results suggest that the yolk sac is the major intermediate erythropoietic and granulopoietic site where expansion and differentiation occur during chicken development. The hepatic hematopoiesis is restricted to the portal spaces and represented by the granulocytic lineage.
authors.affiliation: Laboratory of Pathology, Oswaldo Cruz Institute/Fiocruz, Rio de Janeiro, Brazil.
authors.forename: Priscila Tavares
authors.initials: PT
authors.lastname: Guedes
authors.affiliation: Laboratory of Pathology, Oswaldo Cruz Institute/Fiocruz, Rio de Janeiro, Brazil.
authors.forename: Barbara Cristina Euzébio Pereira Dias
authors.initials: BC
authors.lastname: de Oliveira
authors.affiliation: Laboratory of Pathology, Oswaldo Cruz Institute/Fiocruz, Rio de Janeiro, Brazil.
authors.forename: Pedro Paulo de Abreu
authors.initials: PP
authors.lastname: Manso
authors.affiliation: Laboratory of Pathology, Oswaldo Cruz Institute/Fiocruz, Rio de Janeiro, Brazil.
authors.forename: Luzia Fátima Gonçalves
authors.initials: LF
authors.lastname: Caputo
authors.affiliation: Laboratory of Immunochemistry and Histochemistry, Santa Casa da Misericórdia do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Gerson
authors.initials: G
authors.lastname: Cotta-Pereira
authors.affiliation: Laboratory of Pathology, Oswaldo Cruz Institute/Fiocruz, Rio de Janeiro, Brazil.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Pelajo-Machado
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002642:Chick Embryo; D004920:Erythropoiesis; D006098:Granulocytes / Q000166:cytology; D006410:Hematopoiesis; D008099:Liver / Q000098:blood supply / Q000166:cytology* / Q000196:embryology*; D011169:Portal Vein / Q000166:cytology / Q000196:embryology; D013997:Time Factors; D015017:Yolk Sac / Q000166:cytology* / Q000196:embryology*