title: Platelet activation in cats with hypertrophic cardiomyopathy.
abstract: BACKGROUND
  Cats with hypertrophic cardiomyopathy (HCM) are at risk for development of systemic thromboembolic disease. However, the relationship between platelet activation state and cardiovascular parameters associated with HCM is not well described.
  
  
  OBJECTIVE
  To characterize platelet activation by flow cytometric evaluation of platelet P-selectin and semiquantitative Western blot analysis of soluble platelet-endothelial cell adhesion molecule-1 (sPECAM-1).
  
  
  METHODS
  Eight normal healthy cats (controls) owned by staff and students of the School of Veterinary Medicine and 36 cats from the UC Davis Feline HCM Research Laboratory were studied.
  
  
  METHODS
  Platelet-rich plasma (PRP) was used for all flow cytometry studies. Platelet surface CD41 and P-selectin expression were evaluated before and after ADP stimulation. sPECAM-1 expression was evaluated by Western blot analysis of platelet-poor plasma that had been stabilized with aprotinin. Standard echocardiographic studies were performed.
  
  
  RESULTS
  Resting platelets from cats with severe HCM had increased P-selectin expression compared to controls, and expressed higher surface density of P-selectin reflected by their increased mean fluorescence intensities (MFI). Stimulation with ADP also resulted in significantly increased P-selectin MFI of platelets from cats with severe HCM. Increased P-selectin expression and MFI correlated with the presence of a heart murmur and end-systolic cavity obliteration (ESCO). sPECAM-1 expression from cats with moderate and severe HCM was significantly increased above those of control cats.
  
  
  CONCLUSIONS
  P-selectin and sPECAM expression may be useful biomarkers indicating increased platelet activation in cats with HCM.
authors.affiliation: Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California Davis, Davis, CA.
authors.forename: F
authors.initials: F
authors.lastname: Tablin
authors.forename: T
authors.initials: T
authors.lastname: Schumacher
authors.forename: M
authors.initials: M
authors.lastname: Pombo
authors.forename: C T
authors.initials: CT
authors.lastname: Marion
authors.forename: K
authors.initials: K
authors.lastname: Huang
authors.forename: J W
authors.initials: JW
authors.lastname: Norris
authors.forename: K E
authors.initials: KE
authors.lastname: Jandrey
authors.forename: M D
authors.initials: MD
authors.lastname: Kittleson
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D001792:Blood Platelets / Q000737:chemistry / Q000502:physiology; D015153:Blotting, Western / Q000662:veterinary; D002312:Cardiomyopathy, Hypertrophic / Q000097:blood / Q000000981:diagnostic imaging / Q000503:physiopathology / Q000662:veterinary*; D002371:Cat Diseases / Q000097:blood* / Q000000981:diagnostic imaging / Q000503:physiopathology; D002415:Cats; D005340:Fibrinogen / Q000032:analysis; D005434:Flow Cytometry / Q000662:veterinary; D019007:P-Selectin / Q000097:blood; D015539:Platelet Activation / Q000502:physiology*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000097:blood; D014463:Ultrasonography
chemical_list: D015415:Biomarkers; D019007:P-Selectin; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D005340:Fibrinogen
keywords: Hypertrophic cardiomyopathy; P-selectin; Platelet activation; Platelets; sPECAM-1