title: Embryonic intraventricular exposure to autism-specific maternal autoantibodies produces alterations in autistic-like stereotypical behaviors in offspring mice.
abstract: Multiple studies have implicated a role of maternal autoantibodies reactive against fetal brain proteins specific to autism in the etiology of autism spectrum disorders (ASD). In the current study, we examined the impact of brain-reactive maternal autoantibodies of mothers of children with autism (MAU) on offspring behavior in mice compared to offspring exposed to non-reactive IgG of mothers of typically developing children (MTD). Embryonic offspring were exposed to a single intraventricular injection of MAU or MTD IgG on embryonic day 14. Offspring were allowed to mature to adulthood and were subsequently tested for sociability and stereotypic behaviors using a 3-chambered social approach task, marble burying task, and assessment of spontaneous grooming behaviors in response to a novel environment. Results indicate that MAU offspring display autistic-like stereotypical behavior in both marble burying and spontaneous grooming behaviors. Additionally, small alterations in social approach behavior were also observed in MAU offspring compared to MTD offspring. This report demonstrates for the first time the effects of a single, low dose intraventricular exposure of IgG derived from individual MAU samples on offspring behavior.
authors.affiliation: Department of Pathology and Laboratory Medicine, UC Davis, 95817.|Institute for Pediatric Regenerative Medicine and Shriners Hospitals for Children Northern California, 95817.
authors.forename: Jasmin
authors.initials: J
authors.lastname: Camacho
authors.affiliation: M.I.N.D. Institute, UC Davis, 95817.|Department of Rheumatology/Allergy and Clinical Immunology, UC Davis, 95616.
authors.forename: Karen
authors.initials: K
authors.lastname: Jones
authors.affiliation: Department of Pathology and Laboratory Medicine, UC Davis, 95817.|Institute for Pediatric Regenerative Medicine and Shriners Hospitals for Children Northern California, 95817.
authors.forename: Elaine
authors.initials: E
authors.lastname: Miller
authors.affiliation: Department of Pathology and Laboratory Medicine, UC Davis, 95817.|Institute for Pediatric Regenerative Medicine and Shriners Hospitals for Children Northern California, 95817.
authors.forename: Jeanelle
authors.initials: J
authors.lastname: Ariza
authors.affiliation: M.I.N.D. Institute, UC Davis, 95817.|Department of Psychiatry and Behavioral Sciences, UC Davis, 95817.
authors.forename: Stephen
authors.initials: S
authors.lastname: Noctor
authors.affiliation: M.I.N.D. Institute, UC Davis, 95817.|Department of Rheumatology/Allergy and Clinical Immunology, UC Davis, 95616.
authors.forename: Judy Van
authors.initials: JV
authors.lastname: de Water
authors.affiliation: Department of Pathology and Laboratory Medicine, UC Davis, 95817.|Institute for Pediatric Regenerative Medicine and Shriners Hospitals for Children Northern California, 95817.|M.I.N.D. Institute, UC Davis, 95817.
authors.forename: Verónica
authors.initials: V
authors.lastname: Martínez-Cerdeño
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D001321:Autistic Disorder / Q000276:immunology* / Q000503:physiopathology*; D001323:Autoantibodies / Q000008:administration & dosage*; D003674:Defense Mechanisms; D004195:Disease Models, Animal; D004622:Embryo, Mammalian; D005106:Exploratory Behavior / Q000187:drug effects; D005260:Female; D006801:Humans; D007276:Injections, Intraventricular; D008124:Locomotion / Q000187:drug effects; D008297:Male; D051379:Mice; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000503:physiopathology*; D012919:Social Behavior; D013239:Stereotyped Behavior / Q000187:drug effects*
chemical_list: D001323:Autoantibodies
keywords: Autism spectrum disorders; Immune; In utero exposure; Maternal antibodies; Mouse behavior