title: Anatomical distribution analysis reveals lack of Langerin+ dermal dendritic cells in footpads and tail of C57BL/6 mice.
abstract: Epidermal Langerhans cells (LCs) and dermal dendritic cells (dDCs) capture cutaneous antigens and present them to T-cells in lymph nodes (LNs). The function of LCs and Langerin+ dDCs was extensively studied in the mouse, but their anatomical repartition is unknown. Here, we found LCs in back skin, footpads and tail skin of C57BL/6, BALB/c, 129/Sv and CBA/J mice. Langerin+ dDCs were readily observed in back skin of all strains, but only in footpads and tail of BALB/c and CBA/J mice. Similarly, while LCs were equally present in all LNs and strains, Langerin+ dDCs were found in popliteal LNs (draining footpads) only in BALB/c and CBA/J mice. The sciatic LNs, which we identified as the major tail-draining lymphoid organ, were devoid of Langerin+ dDCs in all strains. Thus, functionally different DCs reside in different skin areas, with variations among mouse strains, implying a potential impact on the cutaneous immune reaction.
authors.affiliation: Laboratory of Immunopathology and Therapeutic Chemistry/Laboratory of Excellence Medalis, CNRS UPR3572, Institut de Biologie Moléculaire et Cellulaire, University of Strasbourg, Strasbourg, France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Voisin
authors.forename: David Gabriel
authors.initials: DG
authors.lastname: Mairhofer
authors.forename: Suzie
authors.initials: S
authors.lastname: Chen
authors.forename: Patrizia
authors.initials: P
authors.lastname: Stoitzner
authors.forename: Christopher George
authors.initials: CG
authors.lastname: Mueller
authors.forename: Vincent
authors.initials: V
authors.lastname: Flacher
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D000951:Antigens, Neoplasm / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism*; D039521:CD11c Antigen / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism*; D000071858:Epithelial Cell Adhesion Molecule; D006614:Hindlimb / Q000378:metabolism*; D007249:Inflammation; D039001:Integrin alpha Chains / Q000378:metabolism; D037181:Lectins, C-Type / Q000378:metabolism*; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008198:Lymph Nodes / Q000473:pathology; D037241:Mannose-Binding Lectins / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D012867:Skin / Q000378:metabolism*; D013623:Tail / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D039521:CD11c Antigen; C498497:Cd207 protein, mouse; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule; D039001:Integrin alpha Chains; D037181:Lectins, C-Type; D037241:Mannose-Binding Lectins; C091367:alpha E integrins; D017493:Leukocyte Common Antigens
keywords: Langerhans cells; dermal dendritic cells; footpad skin; skin-draining lymph nodes; tail skin