title: IFN-τ acts in a dose-dependent manner on prostaglandin production by buffalo endometrial stromal cells cultured in vitro.
abstract: Interferon-τ (IFN-τ) has been recognized as the primary embryonic signal responsible for maternal recognition of pregnancy. Uterine endometrium produces both prostaglandin F2α (PGF2α ) and prostaglandin E2 (PGE2 ). PGF2α is responsible for the luteolysis; however, PGE2 favours establishment of pregnancy by its luteoprotective action. In this study, the dose-response effect of recombinant bovine IFN-τ (rbIFN-τ) on prostaglandin (PG) production by buffalo endometrial stromal cells cultured in vitro was studied. Buffalo endometrial stromal cells were isolated by double enzymatic digestion, initially with trypsin III followed by a cocktail of trypsin III, collagenase type II and DNase I and subsequently cultured till confluence. Further, cells were treated with different doses of rbIFN-τ (0.001, 0.01, 0.1, 1.0 and 10 μg/ml) and keeping a separate set of control. Culture supernatant was collected after 6, 12 and 24 h of treatment. PG levels in the culture supernatant were measured by enzyme immune assay (EIA) and total cellular protein estimated by Bradford method. Results indicated that buffalo endometrial stromal cells following rbIFN-τ treatment enhanced the secretion of both PGE2 and PGF2α , and also its ratio in a strict dose-dependent manner with a significant increase (p < 0.01) in PGE2 production at 1 μg/ml dose of rbIFN-τ and maximal stimulation for both PG was observed at 10 μg/ml. Further, both PG production and its ratio were increased significantly (p < 0.01) in a time-dependent fashion in all the groups at 6, 12 and 24 h post-treatment with highest level achieved at 24 h as compared with control. Absolute levels of PGE2 remained higher than PGF2α indicating PGE2 as the major PG produced by endometrial stromal cells. The dose-dependent response of rbIFN-τ signifies the importance of optimum concentration of IFN-τ for the embryonic development especially during the critical period to establish successful pregnancy.
authors.affiliation: Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, India.
authors.forename: S G
authors.initials: SG
authors.lastname: Chethan
authors.forename: S K
authors.initials: SK
authors.lastname: Singh
authors.forename: J
authors.initials: J
authors.lastname: Nongsiej
authors.forename: H B
authors.initials: HB
authors.lastname: Rakesh
authors.forename: R P
authors.initials: RP
authors.lastname: Singh
authors.forename: N
authors.initials: N
authors.lastname: Kumar
authors.forename: S K
authors.initials: SK
authors.lastname: Agarwal
mesh_terms: D000818:Animals; D002020:Buffaloes; D002417:Cattle; D002478:Cells, Cultured; D015237:Dinoprost / Q000032:analysis / Q000096:biosynthesis; D015232:Dinoprostone / Q000032:analysis / Q000096:biosynthesis; D004717:Endometrium / Q000166:cytology*; D005260:Female; D007370:Interferon Type I / Q000494:pharmacology*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000494:pharmacology*; D011453:Prostaglandins / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000494:pharmacology; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism*
chemical_list: D007370:Interferon Type I; D011257:Pregnancy Proteins; D011453:Prostaglandins; D011994:Recombinant Proteins; C020947:interferon tau; D015237:Dinoprost; D015232:Dinoprostone