title: Production of α- and β-galactosidases from Bifidobacterium longum subsp. longum RD47.
abstract: Approximately 50% of people in the world experience abdominal flatulence after the intake of foods containing galactosides such as lactose or soybean oligosaccharides. The galactoside hydrolyzing enzymes of α- and β-galactosidases have been shown to reduce the levels of galactosides in both the food matrix and the human gastrointestinal tract. This study aimed to optimize the production of α- and β-galactosidases of Bifidobacterium longum subsp. longum RD47 with a basal medium containing whey and corn steep liquor. The activities of both enzymes were determined after culturing at 37°C at pH 6.0 for 30 h. The optimal production of α- and β-galactosidases was obtained with soybean oligosaccharides as a carbon source and proteose peptone no. 3 as a nitrogen source. The optimum pH for both α- and β-galactosidases was 6.0. The optimum temperatures were 35°C for α-galactosidase and 37°C for β- galactosidase. They showed temperature stability up to 37°C . At a 1 mM concentration of metal ions, CuSO4 inhibited the activities of α- and β-galactosidases by 35% and 50%, respectively. On the basis of the results obtained in this study, B. longum RD47 may be used for the production of α- and β-galactosidases, which may reduce the levels of flatulence factors.
authors.affiliation: Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Yoo Ri
authors.initials: YR
authors.lastname: Han
authors.forename: So Youn
authors.initials: SY
authors.lastname: Youn
authors.forename: Geun Eog
authors.initials: GE
authors.lastname: Ji
authors.forename: Myeong Soo
authors.initials: MS
authors.lastname: Park
mesh_terms: D001644:Bifidobacterium / Q000254:growth & development / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D004789:Enzyme Activation; D005697:Galactosides / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007477:Ions; D008670:Metals; D009584:Nitrogen / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism; D013025:Glycine max / Q000737:chemistry; D013379:Substrate Specificity; D013696:Temperature; D000519:alpha-Galactosidase / Q000096:biosynthesis*; D001616:beta-Galactosidase / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D005697:Galactosides; D007477:Ions; D008670:Metals; D009844:Oligosaccharides; D002244:Carbon; D000519:alpha-Galactosidase; D001616:beta-Galactosidase; D009584:Nitrogen