title: Loop-mediated isothermal amplification test for detection of Neisseria gonorrhoeae in urine samples and tolerance of the assay to the presence of urea.
abstract: A loop-mediated isothermal amplification (LAMP) assay for open reading frame 1 (ORF1) of the glutamine synthetase gene of Neisseria gonorrhoeae was able to tolerate urea concentrations of ≤ 1.8 M, compared with a PCR assay that was functional at concentrations of <100 mM. The LAMP assay was as sensitive as the PCR assay while being faster and simpler to perform.
authors.affiliation: School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool, United Kingdom.
authors.forename: Thomas
authors.initials: T
authors.lastname: Edwards
authors.affiliation: School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool, United Kingdom.
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Burke
authors.affiliation: School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool, United Kingdom.
authors.forename: Helen B
authors.initials: HB
authors.lastname: Smalley
authors.affiliation: Mast Group Ltd., Bootle, Liverpool, United Kingdom.
authors.forename: Liz
authors.initials: L
authors.lastname: Gillies
authors.affiliation: School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool, United Kingdom G.Hobbs@ljmu.ac.uk.
authors.forename: Glyn
authors.initials: G
authors.lastname: Hobbs
mesh_terms: D004791:Enzyme Inhibitors / Q000378:metabolism*; D005974:Glutamate-Ammonia Ligase / Q000235:genetics; D006069:Gonorrhea / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D013696:Temperature; D013997:Time Factors; D014508:Urea / Q000378:metabolism*; D014556:Urine / Q000737:chemistry / Q000382:microbiology*
chemical_list: D004791:Enzyme Inhibitors; D014508:Urea; D005974:Glutamate-Ammonia Ligase