title: Fluid flow modulates the expression of genes involved in the Wnt signaling pathway in osteoblasts in 3D culture conditions.
abstract: The balance between osteoclastic bone resorption and osteoblastic bone formation maintains bone mass, while mechanical loads stimulate bone formation and suppress resorption. The molecular mechanisms responsible for this process have not yet been fully elucidated. In the present study, we assessed whether mechanical stimulation by pulsating fluid flow (PFF) leads to functional Wnt production and affects the function of osteoblasts. ROS17/2.8 osteoblasts were submitted to 1-4 h PFF (0.8 Pa) by three-dimensional (3D) cell culture system with fluid flow. PFF upregulated the gene expression levels of adenomatous polyposis coli, alkaline phosphatase, low density lipoprotein receptor-related protein 5 (LRP5), Wnt3a and β-catenin [catenin beta 1 (CTNNB1)] in all the groups of osteoblasts. Our results suggest that mechanical stimulation by PFF induces the differentiation of osteoblasts and the activation of the Wnt/β-catenin signaling pathway in a 3D cell culture system. Furthermore, mechanical stress plays an important role in the Wnt/β-catenin signaling pathway and is involved in bone formation.
authors.affiliation: State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Jia
authors.affiliation: Department of Stomatology, The People's Hospital of Deyang City, Deyang, Sichuan 618000, P.R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.affiliation: State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Geng
authors.affiliation: State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Gong
authors.affiliation: State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Shi-Jie
authors.initials: SJ
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Ling-Xian
authors.initials: LX
authors.lastname: Meng
authors.affiliation: State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Lan
authors.affiliation: State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Ban
mesh_terms: D002460:Cell Line; D006801:Humans; D060492:Low Density Lipoprotein Receptor-Related Protein-5 / Q000378:metabolism; D010006:Osteoblasts / Q000378:metabolism*; D013314:Stress, Mechanical; D060449:Wnt Signaling Pathway / Q000502:physiology*; D060509:Wnt3A Protein / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism
chemical_list: C495270:CTNNB1 protein, human; D060492:Low Density Lipoprotein Receptor-Related Protein-5; D060509:Wnt3A Protein; D051176:beta Catenin