title: Biological activity of lactoferrin-functionalized biomimetic hydroxyapatite nanocrystals.
abstract: The emergence of bacterial strains resistant to antibiotics is a general public health problem. Progress in developing new molecules with antimicrobial properties has been made. In this study, we evaluated the biological activity of a hybrid nanocomposite composed of synthetic biomimetic hydroxyapatite surface-functionalized by lactoferrin (LF-HA). We evaluated the antimicrobial, anti-inflammatory, and antioxidant properties of LF-HA and found that the composite was active against both Gram-positive and Gram-negative bacteria, and that it modulated proinflammatory and anti-inflammatory responses and enhanced antioxidant properties as compared with LF alone. These results indicate the possibility of using LF-HA as an antimicrobial system and biomimetic hydroxyapatite as a candidate for innovative biomedical applications.
authors.affiliation: Department of Agriculture Special Biotechnology Center Federico II, CeBIOTEC Biotechnology, University of Naples Federico II, Naples, Italy.
authors.forename: Nunzia
authors.initials: N
authors.lastname: Nocerino
authors.affiliation: Department of Agriculture Special Biotechnology Center Federico II, CeBIOTEC Biotechnology, University of Naples Federico II, Naples, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Fulgione
authors.affiliation: Department of Agriculture Special Biotechnology Center Federico II, CeBIOTEC Biotechnology, University of Naples Federico II, Naples, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Iannaccone
authors.affiliation: Department of Agriculture Special Biotechnology Center Federico II, CeBIOTEC Biotechnology, University of Naples Federico II, Naples, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Tomasetta
authors.affiliation: Department of Agriculture Special Biotechnology Center Federico II, CeBIOTEC Biotechnology, University of Naples Federico II, Naples, Italy.
authors.forename: Flora
authors.initials: F
authors.lastname: Ianniello
authors.affiliation: Department of Agriculture Special Biotechnology Center Federico II, CeBIOTEC Biotechnology, University of Naples Federico II, Naples, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Martora
authors.affiliation: Department of Chemistry, G Ciamician, Alma Mater Studiorum, University of Bologna, Bologna, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Lelli
authors.affiliation: Department of Chemistry, G Ciamician, Alma Mater Studiorum, University of Bologna, Bologna, Italy.
authors.forename: Norberto
authors.initials: N
authors.lastname: Roveri
authors.affiliation: Department of Food Inspection IZS ME, Naples, Italy.
authors.forename: Federico
authors.initials: F
authors.lastname: Capuano
authors.affiliation: Department of Agriculture Special Biotechnology Center Federico II, CeBIOTEC Biotechnology, University of Naples Federico II, Naples, Italy.
authors.forename: Rosanna
authors.initials: R
authors.lastname: Capparelli
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D040761:Biomimetic Materials / Q000737:chemistry* / Q000494:pharmacology*; D002417:Cattle; D045744:Cell Line, Tumor; D017886:Durapatite / Q000737:chemistry; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006801:Humans; D007155:Immunologic Factors / Q000737:chemistry / Q000494:pharmacology; D007781:Lactoferrin / Q000737:chemistry* / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D053761:Nanocomposites / Q000737:chemistry; D050997:Nanomedicine; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D000975:Antioxidants; D007155:Immunologic Factors; D017886:Durapatite; D007781:Lactoferrin
keywords: biological activity; biomimetism; drug delivery; hydroxyapatite nanocrystals; lactoferrin