title: Enzymatic mineralization of silk scaffolds.
abstract: The present study focuses on the alkaline phosphatase (ALP) mediated formation of apatitic minerals on porous silk fibroin protein (SFP) scaffolds. Porous SFP scaffolds impregnated with different concentrations of ALP are homogeneously mineralized under physiological conditions. The mineral structure is apatite while the structures differ as a function of the ALP concentration. Cellular adhesion, proliferation, and colonization of osteogenic MC3T3 cells improve on the mineralized SFP scaffolds. These findings suggest a simple process to generate mineralized scaffolds that can be used to enhanced bone tissue engineering-related utility.
authors.affiliation: Polymer Chemistry and Biomaterials Research Group, Department of Organic Chemistry, Ghent University, Krijgslaan 281/S4-Bis, B-9000, Ghent, Belgium; Department of Biomedical Engineering, 4 Colby Street, Tufts University, Medford, MA, 02155, USA. sksamalrec@gmail.com.
authors.forename: Sangram K
authors.initials: SK
authors.lastname: Samal
authors.forename: Mamoni
authors.initials: M
authors.lastname: Dash
authors.forename: Heidi A
authors.initials: HA
authors.lastname: Declercq
authors.forename: Tom
authors.initials: T
authors.lastname: Gheysens
authors.forename: Jolien
authors.initials: J
authors.lastname: Dendooven
authors.forename: Pascal
authors.initials: P
authors.lastname: Van Der Voort
authors.forename: Ria
authors.initials: R
authors.lastname: Cornelissen
authors.forename: Peter
authors.initials: P
authors.lastname: Dubruel
authors.forename: David L
authors.initials: DL
authors.lastname: Kaplan
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D012831:Bombyx; D002130:Calcium Phosphates / Q000378:metabolism; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D005349:Fibroins / Q000737:chemistry / Q000494:pharmacology / Q000648:ultrastructure; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008903:Minerals / Q000378:metabolism*; D008970:Molecular Weight; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D047011:Silk / Q000378:metabolism* / Q000494:pharmacology / Q000648:ultrastructure; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman; D013818:Thermogravimetry; D054457:Tissue Scaffolds / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D002130:Calcium Phosphates; D008903:Minerals; D047011:Silk; D005349:Fibroins; C020243:calcium phosphate; D000469:Alkaline Phosphatase
keywords: alkaline phosphatase; mineralization; osteogenesis; scaffolds; silk