title: [Establishment of a genetic transformation system for Penicillium brevicompactum to produce mycophenolic acid].
abstract: OBJECTIVE
  We established a genetic transformation system for Penicillium brevicompactum to produce mycophenolic acid.
  
  
  METHODS
  We developed protoplast transformation methods mediated by Polyethylene glycol, using phleomycin resistance gene (Sh ble) as a dominant selection marker.
  
  
  RESULTS
  The frequency of transformation was up to 2 - 3 transformants per microg DNA; analysis of the transformants by PCR showed that the foreign DNA had been integrated into the host genome. The transformants retained stable after generation.
  
  
  CONCLUSIONS
  The establishment of the genetic transformation system of Penicillium brevicompactum could serve as the basis for the research of molecular biology and the breeding of gene engineering of the fungus.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Ren
authors.forename: Caiyun
authors.initials: C
authors.lastname: Su
authors.forename: Jing
authors.initials: J
authors.lastname: Yan
authors.forename: Meng
authors.initials: M
authors.lastname: Dai
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhao
authors.forename: Hongyi
authors.initials: H
authors.lastname: Wang
authors.forename: Mingwei
authors.initials: M
authors.lastname: Dai
authors.forename: Jia
authors.initials: J
authors.lastname: Zhang
mesh_terms: D009173:Mycophenolic Acid / Q000096:biosynthesis*; D010407:Penicillium / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic
chemical_list: D009173:Mycophenolic Acid