title: Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines.
abstract: Analysis of complete genome sequences of three Slovak isolates of grapevine Pinot gris virus (GPGV) showed their low heterogeneity (reaching 1.7 %) and a close relationship to the Italian NC_015782 isolate (4.2-4.5 % divergence). Comparison of Slovak and Italian isolates revealed an unusual accumulation of 21 indel mutations in ORF1, resulting in a localized high divergence in the encoded amino acid sequences. An elevated divergence in the 5' extremity of the GPGV genomes is suggestive of a recombination between Slovak isolates and grapevine berry inner necrosis virus. RT-PCR allowed the frequent detection of closely related GPGV isolates in grapevines from Slovakia and the Czech Republic.
authors.affiliation: Department of Plant Virology, Institute of Virology, Slovak Academy of Sciences, Dúbravská cesta 9, 84505, Bratislava, Slovakia, Miroslav.Glasa@savba.sk.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Glasa
authors.forename: Lukáš
authors.initials: L
authors.lastname: Predajňa
authors.forename: Petr
authors.initials: P
authors.lastname: Komínek
authors.forename: Alžbeta
authors.initials: A
authors.lastname: Nagyová
authors.forename: Thierry
authors.initials: T
authors.lastname: Candresse
authors.forename: Antonio
authors.initials: A
authors.lastname: Olmos
mesh_terms: D018153:Czech Republic; D052677:Flexiviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D014644:Genetic Variation; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D018154:Slovakia; D027843:Vitis / Q000821:virology*