title: [Correlations between tumor infiltrating Th17 cells and clinicopathological parameters in patients with colorectal cancer].
abstract: OBJECTIVE
  To observe the correlations between the percentage of tumor infiltrating Th17 cells and clinicopathological parameters in patients with colorectal cancer (CRC).
  
  
  METHODS
  Tumor and normal mucosal tissues were collected from 28 CRC patients. The proportions of Th17 cells in lymphocytes were detected by flow cytometry. The supernatant levels of IL-17A in cultured normal and tumor tissues were measured by ELISA. The correlations between the proportions of tumor infiltrating Th17 cells as well as the tumor supernatant levels of IL-17A and clinicopathological parameters in CRC patients were further analyzed.
  
  
  RESULTS
  There was no significant difference in the expression of Th17 cells between normal tissues (2.24 ± 0.24)% and tumor tissues (2.47 ± 0.34)% (P>0.05). Compared with normal tissues, the supernatant levels of IL-17A in tumor tissues were significantly up-regulated [(257.74±31.36) pg/mL vs (163.53±12.62)pg/mL, P<0.05]. The proportions of tumor infiltrating Th17 cells and the tumor supernatant levels of IL-17A had no associations with age, gender, tumor location, tumor size and growth characteristics (P>0.05), but they were correlated with tumor differentiation, lymph node metastasis and TNM stage (P<0.05). No associations between the proportions of infiltrating Th17 cells as well as the supernatant levels of IL-17A in the normal tissues and all clinicopathological parameters were found (P>0.05).
  
  
  CONCLUSIONS
  Th17 cells may be involved in the immunopathogenesis of the development of CRC.
authors.affiliation: Department of Clinical Laboratory, Hebei General Hospital, Shijiazhuang 050051, China.
authors.forename: Jiansheng
authors.initials: J
authors.lastname: Wang
authors.forename: Lizhi
authors.initials: L
authors.lastname: Zhang
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Tie
authors.forename: Yuanpeng
authors.initials: Y
authors.lastname: Lv
authors.forename: Jinxiao
authors.initials: J
authors.lastname: Song
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015179:Colorectal Neoplasms / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D005260:Female; D006801:Humans; D020381:Interleukin-17 / Q000097:blood; D008297:Male; D008875:Middle Aged; D058504:Th17 Cells / Q000276:immunology*
chemical_list: D020381:Interleukin-17