title: [Acyldepsipeptide 1 induces apoptosis in renal cancer cells by down-regulation of Gli and Bcl-2 via SHH pathway].
abstract: OBJECTIVE
  To investigate the effects of acyldepsipeptide 1 (ADEP1) on renal cancer cell apoptosis and on the expressions of SHH signaling pathway related protein Gli-1 and Bcl-2.
  
  
  METHODS
  The renal cancer cells (ACHN and 769-P) were treated with 50 μmol/L ADEP1 for 48 hours. The changes of cell morphology and the nucleus shape in cell apoptosis were observed under a light microscope, and cell viability was measured by MTT assay. The mRNA levels of SHH and Gli-1 were detected by real-time quantitative PCR (qRT-PCR), and the protein levels of Gli-1 and Bcl-2 were determined by Western blotting.
  
  
  RESULTS
  ADEP1 induced renal cancer cell apoptosis through reducing the transcription of SHH and Gli-1, and decreasing Gli-1 and Bcl-2 protein expressions.
  
  
  CONCLUSIONS
  ADEP1 could induce renal cancer cell apoptosis by the inhibition on SHH signaling pathway related proteins.
authors.affiliation: Department of Urology, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Xu
authors.affiliation: Department of Urology, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Chong
authors.initials: C
authors.lastname: DU
authors.affiliation: Department of Urology, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Zhenkun
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Department of Urology, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Urology, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Shi
authors.affiliation: Department of Urology, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Xinyang
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Hailong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Urology, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Dalin
authors.initials: D
authors.lastname: He
authors.affiliation: Department of Urology, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Guo
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D047630:Depsipeptides / Q000494:pharmacology*; D015536:Down-Regulation / Q000187:drug effects; D053823:Hedgehog Proteins / Q000502:physiology*; D006801:Humans; D007680:Kidney Neoplasms / Q000188:drug therapy* / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis / Q000037:antagonists & inhibitors*; D015398:Signal Transduction / Q000187:drug effects* / Q000502:physiology; D014157:Transcription Factors / Q000037:antagonists & inhibitors* / Q000235:genetics; D000071676:Zinc Finger Protein GLI1
chemical_list: C584118:ADEP1 compound; D047630:Depsipeptides; C400551:GLI1 protein, human; D053823:Hedgehog Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; C509239:SHH protein, human; D014157:Transcription Factors; D000071676:Zinc Finger Protein GLI1