title: Androgenesis in chickpea: anther culture and expressed sequence tags derived annotation.
abstract: Double haploid technique is not routinely used in legume breeding programs, though recent publications report haploid plants via anther culture in chickpea (Cicer arietinum L.). The focus of this study was to develop an efficient and reproducible protocol for the production of double haploids with the application of multiple stress pre-treatments such as centrifugation and osmotic shock for genotypes of interest in chickpea for their direct use in breeding programs. Four genotypes, ICC 4958, WR315, ICCV 95423 and Arearti were tested for anther culture experiments. The yield was shown to be consistent with 3-5 nucleate microspores and 2-7 celled structures with no further growth. To gain a further insight into the molecular mechanism underlying the switch from microsporogenesis to androgenesis, bioinformatics tools were employed. The challenges on the roles of such genes were reviewed while an attempt was made to find putative candidates for androgenesis using Expressed Sequenced Tags (EST) and interolog based protein interaction analyses.
authors.affiliation: International Crop Research Institute for the Semi-Arid Tropics, Patancheru 502 319, India. sameera.panchangam@gmail.com
authors.forename: Sameera Sastry
authors.initials: SS
authors.lastname: Panchangam
authors.affiliation: International Crop Research Institute for the Semi-Arid Tropics, Patancheru 502 319, India.
authors.forename: Nalini
authors.initials: N
authors.lastname: Mallikarjuna
authors.affiliation: International Crop Research Institute for the Semi-Arid Tropics, Patancheru 502 319, India.
authors.forename: Pooran M
authors.initials: PM
authors.lastname: Gaur
authors.affiliation: Bioclues Organization, IKP Knowledge Park, Picket, Secunderabad 500 009, India.
authors.forename: Prashanth
authors.initials: P
authors.lastname: Suravajhala
mesh_terms: D001947:Breeding; D029863:Cicer / Q000235:genetics*; D019295:Computational Biology; D020224:Expressed Sequence Tags; D007887:Fabaceae / Q000235:genetics; D005838:Genotype; D006238:Haploidy; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D060066:Protein Interaction Maps / Q000235:genetics; D013312:Stress, Physiological