title: Strategies for measurement of biotransformation enzyme gene expression.
abstract: The analysis of gene expression is an integral part of any gene function research. A wide variety of techniques have been developed for this purpose, each with its own advantages and limitations. The following chapter seeks to provide an overview of some of the most recent as well as conventional methods to study gene expression. These approaches include Northern blot analysis, ribonuclease protection assay, reverse transcription polymerase chain reaction, expressed tag sequencing, differential display, cDNA arrays, serial analysis of gene expression, and transcriptome sequencing. The current applications of the information derived from gene expression studies require most of the assays to be adaptable for the quantitative analysis of a large number of samples and endpoints within a short period of time coupled with cost-effectiveness. A comparison of some of these features of each analytical approach as well as their advantages and disadvantages has also been provided.
authors.affiliation: Division of Hematology/Oncology, Department of Medicine, Hillman Cancer Center, 5117 Centre Avenue, Suite 1.32e, Pittsburgh, PA, 15213, USA, romkes@pitt.edu.
authors.forename: Marjorie
authors.initials: M
authors.lastname: Romkes
authors.forename: Shama C
authors.initials: SC
authors.lastname: Buch
mesh_terms: D000818:Animals; D001711:Biotransformation; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D003577:Cytochrome P-450 Enzyme System