title: Association of the g.27563G>A osteoprotegerin genetic polymorphism with bone mineral density in Chinese women.
abstract: Osteoporosis is a common multifactorial disease in postmenopausal women. This study aimed to investigate the association of the g.27563G>A osteoprotegerin (OPG) genetic polymorphism with bone mineral density (BMD) and osteoporosis. A case-control study was carried out with 435 osteoporosis postmenopausal women cases and 442 age-matched healthy controls. The BMD at the femoral neck hip, lumbar spine (L₂₋₄), and total hip were assessed by Norland XR-46 dual-energy X-ray absorptiometry. The genotypes of the g.27563G>A genetic polymorphism were detected by created restriction site-polymerase chain reaction and verified by DNA sequencing methods. We detected that the g.27563G>A genetic polymorphism was a non-synonymous mutation that resulted in an arginine (Arg) to glutamine (Gln) amino acid replacement (p.Arg333Gln). Significant differences were found in the BMD of the femoral neck hip, lumbar spine (L₂₋₄), and total hip among different genotypes of the g.27563G>A genetic polymorphism. Subjects with the genotype GG had significantly higher BMD values than those with genotypes GA and AA (P < 0.05). Our data indicated that the A allele of the g.27563G>A genetic polymorphism in OPG could be associated with lower BMD values in the Chinese postmenopausal women evaluated, and that it might be an increased risk factor for osteoporosis.
authors.affiliation: Dalian University of Technology, Dalian, Liaoning Province, China.
authors.forename: Y P
authors.initials: YP
authors.lastname: Liu
authors.affiliation: Department of Orthopaedic Surgery, The Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning Province, China.
authors.forename: D W
authors.initials: DW
authors.lastname: Zhao
authors.affiliation: Department of Orthopaedic Surgery, The Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning Province, China.
authors.forename: W M
authors.initials: WM
authors.lastname: Wang
authors.affiliation: Dalian University of Technology, Dalian, Liaoning Province, China.
authors.forename: B J
authors.initials: BJ
authors.lastname: Wang
authors.affiliation: Dalian University of Technology, Dalian, Liaoning Province, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Dalian University of Technology, Dalian, Liaoning Province, China.
authors.forename: Z G
authors.initials: ZG
authors.lastname: Li
mesh_terms: D000368:Aged; D000483:Alleles; D044466:Asian People; D015519:Bone Density / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D005260:Female; D005272:Femur Neck / Q000473:pathology; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008875:Middle Aged; D015663:Osteoporosis, Postmenopausal / Q000235:genetics* / Q000473:pathology; D053244:Osteoprotegerin / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide
chemical_list: D053244:Osteoprotegerin