title: A tissue-specific gene expression template portrays heart development and pathology.
abstract: Congenital heart defects (CHD) are the most common cause of death in children under the age of 1. Tetralogy of Fallot (TOF) is a severe CHD that results from developmental defects in the conotruncal outflow tract. Recently, a tissue-specific gene expression template (GET) was derived from microarray data that accurately characterized multiple normal human tissues. We used the GET to examine spatial, temporal, and a pathological condition (TOF) within a single organ, the heart. The GET, as previously defined, generally identified temporal and spatial differences in the cardiac tissue. Differences in the stoichiometry of the GET reflected the severe developmental disturbance associated with TOF. Our analysis suggests that the homoeostatic equilibrium assessed by the GET at the inter-organ level is generally maintained at the intra-organ level as well.
authors.forename: Amy
authors.initials: A
authors.lastname: Rodemoyer
authors.forename: Nataliya
authors.initials: N
authors.lastname: Kibiryeva
authors.forename: Alexis
authors.initials: A
authors.lastname: Bair
authors.forename: Jennifer
authors.initials: J
authors.lastname: Marshall
authors.forename: James E
authors.initials: JE
authors.lastname: O'Brien
authors.affiliation: The Ward Family Heart Center, Children's Mercy Hospitals and Clinics, Kansas City, MO 64108, USA. dbittel@cmh.edu.
authors.forename: Douglas C
authors.initials: DC
authors.lastname: Bittel
mesh_terms: D000328:Adult; D002648:Child; D002675:Child, Preschool; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000254:growth & development*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008875:Middle Aged; D009206:Myocardium / Q000378:metabolism*; D009928:Organ Specificity; D013771:Tetralogy of Fallot / Q000235:genetics* / Q000473:pathology