title: Estimating telomere length from whole genome sequence data.
abstract: Telomeres play a key role in replicative ageing and undergo age-dependent attrition in vivo. Here, we report a novel method, TelSeq, to measure average telomere length from whole genome or exome shotgun sequence data. In 260 leukocyte samples, we show that TelSeq results correlate with Southern blot measurements of the mean length of terminal restriction fragments (mTRFs) and display age-dependent attrition comparably well as mTRFs.
authors.affiliation: Genome Informatics, Wellcome Trust Sanger Institute, Hinxton, CB10 1SA, UK.
authors.forename: Zhihao
authors.initials: Z
authors.lastname: Ding
authors.affiliation: Department of Twin Research and Genetic Epidemiology, King's College London, London, WC2R 2LS, UK.
authors.forename: Massimo
authors.initials: M
authors.lastname: Mangino
authors.affiliation: The Center for Human Development and Aging, New Jersey Medical School, Rutgers University, Newark, NJ 07103, USA.
authors.forename: Abraham
authors.initials: A
authors.lastname: Aviv
authors.affiliation: Department of Twin Research and Genetic Epidemiology, King's College London, London, WC2R 2LS, UK.
authors.forename: Tim
authors.initials: T
authors.lastname: Spector
authors.affiliation: Genome Informatics, Wellcome Trust Sanger Institute, Hinxton, CB10 1SA, UK rd@sanger.ac.uk.
authors.forename: Richard
authors.initials: R
authors.lastname: Durbin
mesh_terms: D000328:Adult; D000368:Aged; D001483:Base Sequence; D003430:Cross-Sectional Studies; D059472:Exome; D015894:Genome, Human; D006801:Humans; D007962:Leukocytes / Q000502:physiology; D008875:Middle Aged; D008957:Models, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D012984:Software; D016615:Telomere / Q000235:genetics*; D059505:Telomere Homeostasis