title: Association of benign prostate hyperplasia with polymorphisms in VDR, CYP17, and SRD5A2 genes among Lebanese men.
abstract: BACKGROUND
  The aim of the study was to investigate any associations between benign prostate hyperplasia (BPH) and single nucleotide polymorphisms (SNPs) in the VDR gene (FokI, BsmI, ApaI and TaqαI loci) and the CYP17 gene (MspA1I locus), as well as TA repeat polymorphism in SRD5A2 gene among Lebanese men.
  
  
  METHODS
  DNA extracted from blood of 68 subjects with confirmed BPH and 79 age-matched controls was subjected to PCR/PCR-restriction fragment length polymorphism analysis. The odds ra=tio (OR) of having a genotype and the relative risk (RR) of developing BPH for having the genotype were calculated and the alleles were designated risk-bearing or protective.
  
  
  RESULTS
  Our data indicated that the A and B alleles of the VDR ApaI and BsmI SNPs were highly associated with increased risk of BPH (p=0.0168 and 0.0002, respectively). Moreover, 63% of the controls compared to 43% of the subjects with BPH were homozygous for none of the risk-bearing alleles (p=0.0123) whereas 60% of the controls and 28% of the subjects with BPH were homozygous for two or more protective alleles (p<0.0001).
  
  
  CONCLUSIONS
  For the first time, our study demonstrated that ApaI and BsmI of the VDR gene are associated with risk of BPH among Lebanese men. Our study also indicated that overall polymorphism profile of all the genes involved in prostate physiology could be a better predictor of BPH risk.
authors.affiliation: Radioimmunoassay Laboratory, Lebanese Atomic Energy Commission, Beirut, Lebanon E-mail : ichheisenana@gmail.com.
authors.forename: Asmahan Ali
authors.initials: AA
authors.lastname: El Ezzi
authors.forename: Wissam Rateeb
authors.initials: WR
authors.lastname: Zaidan
authors.forename: Mohammed Ahmed
authors.initials: MA
authors.lastname: El-Saidi
authors.forename: Nabil
authors.initials: N
authors.lastname: Al-Ahmadieh
authors.forename: Jeffrey Benjamin
authors.initials: JB
authors.lastname: Mortenson
authors.forename: Ruhul Haque
authors.initials: RH
authors.lastname: Kuddus
mesh_terms: D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000235:genetics*; D000368:Aged; D000483:Alleles; D001483:Base Sequence; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007861:Lebanon; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011467:Prostate / Q000473:pathology; D011470:Prostatic Hyperplasia / Q000235:genetics*; D018167:Receptors, Calcitriol / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013254:Steroid 17-alpha-Hydroxylase / Q000235:genetics*
chemical_list: D008565:Membrane Proteins; D018167:Receptors, Calcitriol; C579457:VDR protein, human; C501892:CYP17A1 protein, human; D013254:Steroid 17-alpha-Hydroxylase; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase; C548468:SRD5A2 protein, human