title: Sperm DNA fragmentation measured by Halosperm does not impact on embryo quality and ongoing pregnancy rates in IVF/ICSI treatments.
abstract: Sperm DNA fragmentation (SDF) has been proposed to be one of the main markers regarding male infertility. A prospective study was performed to assess primarily whether sperm DNA damage has any impact on embryological data and secondarily on pregnancy rates. This prospective study evaluated the sperm DNA damage in fresh ejaculated sperm samples from couples undergoing IVF/ICSI treatments, using the improved SCD method, known as Halosperm(®) . The results were evaluated by performing statistical analysis with the statistical package of SPSS v17. A total of 156 fresh semen samples derived from 156 couples undergoing 156 IVF/ICSI cycles. From the 156 couples, 139 finally reached the embryo transfer (ET) procedure. Overall, SDF did not correlate with embryological data, while ongoing pregnancy rate/ET was 21.6%. SDF only correlated with sperm characteristics. After the categorisation of SDF (≤35% and >35%), according to the specific references of the method used, embryological data were comparable as also ongoing pregnancy rates. Using the SCD method, sperm DNA damage is associated neither with embryological data nor to pregnancy rates. However, we should not rule out the fact that extremely high DNA damages are associated with total pregnancy failure.
authors.affiliation: Department of Obstetrics and Gynaecology, School of Health Sciences, Faculty of Medicine, University of Thessalia, Larissa, Greece.
authors.forename: G
authors.initials: G
authors.lastname: Anifandis
authors.forename: T
authors.initials: T
authors.lastname: Bounartzi
authors.forename: C I
authors.initials: CI
authors.lastname: Messini
authors.forename: K
authors.initials: K
authors.lastname: Dafopoulos
authors.forename: R
authors.initials: R
authors.lastname: Markandona
authors.forename: S
authors.initials: S
authors.lastname: Sotiriou
authors.forename: A
authors.initials: A
authors.lastname: Tzavella
authors.forename: I E
authors.initials: IE
authors.lastname: Messinis
mesh_terms: D053938:DNA Fragmentation; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007248:Infertility, Male / Q000235:genetics* / Q000378:metabolism / Q000628:therapy*; D008297:Male; D011247:Pregnancy; D018873:Pregnancy Rate; D011446:Prospective Studies; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000378:metabolism*
keywords: Embryo quality; pregnancy; sperm DNA fragmentation; sperm parameters