title: Immune-related chemotactic factors were found in acute coronary syndromes by bioinformatics.
abstract: DNA microarray data for thrombus-related leukocyte from patients with acute coronary syndrome (ACS) was analyzed to acquire key genes associated with ACS. Microarray data set GSE19339, including four ACS patients' samples and four normal samples, were downloaded from Gene Expression Omnibus database. Raw data was pre-processed and differentially expressed genes (DEGs) were identified by Affy packages of R. The interaction network was established with STRING. DrugBank was retrieved to obtain relevant small molecules. A total of 487 differentially expressed genes were identified as DEGs between normal and disease samples. Among which, ten up-regulated genes belonging to chemokine family (CCL2, CCR1, CXCL3, CXCL2, CCL8, CXCL11, CCL7, IL10, CCL22 and CCL20) were related to inflammatory response. In addition, two inhibitors of CCL2 (L-Mimosine) were retrieved from the DrugBank database. Considering the roles of inflammatory response in the progression of ACS and the functions of the ten up-regulated genes, we speculated that these genes might be related to ACS. Moreover, the inhibitors could provide guidelines for future drug design acting on these genes.
authors.affiliation: Department of Special Needs Medical Branch, Shanghai Tongji Hospital, School of Medicine, Tongji University, No. 389 Xincun Road, Putuo District, Shanghai, 200065, China, leizhanglzh@hotmail.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Aibin
authors.initials: A
authors.lastname: Liang
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Bing
authors.initials: B
authors.lastname: Deng
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
mesh_terms: D054058:Acute Coronary Syndrome / Q000188:drug therapy / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D002316:Cardiotonic Agents / Q000494:pharmacology; D016022:Case-Control Studies; D018925:Chemokines / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000276:immunology; D019295:Computational Biology; D003613:Danazol / Q000494:pharmacology; D030541:Databases, Genetic; D062313:Databases, Pharmaceutical; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000276:immunology*; D006801:Humans; D008898:Mimosine / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis; D025941:Protein Interaction Mapping; D054852:Small Molecule Libraries / Q000494:pharmacology
chemical_list: D002316:Cardiotonic Agents; D018925:Chemokines; D054852:Small Molecule Libraries; D008898:Mimosine; D003613:Danazol