title: Proteomic analysis during ontogenesis of secondary xylem in maritime pine.
abstract: Secondary xylem (wood) is formed through an intricate biological process that results in a highly variable final product. Studies have focused on understanding the molecular events for wood formation in conifers. In this process environmental, ontogenic and genetic factors influence variation in wood characteristics, including anatomical, chemical and physical properties. The main objective of this study was to analyse the ageing (ontogenic) effect on protein accumulation in wood-forming tissues along a cambial age (CA) gradient, ranging from juvenile wood (JW) sampled at the top of the tree, to mature wood (MW) sampled at the bottom of the tree. A total of 62 proteins whose accumulation varied by at least 1.5-fold according to CA were selected and identified by ESI-MS/MS; 30 of these were more abundant in MW and 32 were more abundant in JW. Consistent with earlier findings, our results show that JW is a tissue characterized by a high energy demand with the accumulation of gene products involved in energy, protein fate and cellular transport, while proteins identified in MW (heat shock response, oxygen and radical detoxification, and the S-adenosyl methionine cycle) support the idea that this tissue undergoes extended cell-wall thickening and a delay of programmed cell death.
authors.affiliation: INRA, UMR 1202 BIOGECO, F-33610 Cestas, France Instituto de Biología Vegetal y Biotecnología, Universidad de Talca, 2 Norte 685, Talca, Chile Present address: Center of Plant, Soil Interaction and Natural Resources Biotechnology, BIOREN, Universidad de la Frontera, PO Box 54-D, Temuco, Chile.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Garcés
authors.affiliation: INRA, UMR 1202 BIOGECO, F-33610 Cestas, France Université de Bordeaux, BIOGECO, UMR 1202, F-33170 Talence, France.
authors.forename: Grégoire
authors.initials: G
authors.lastname: Le Provost
authors.affiliation: INRA, UMR 1202 BIOGECO, F-33610 Cestas, France Université de Bordeaux, BIOGECO, UMR 1202, F-33170 Talence, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Lalanne
authors.affiliation: Centre Génomique Fonctionnelle de Bordeaux, Université de Bordeaux, Plateforme Protéome, F-33000 Bordeaux, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Claverol
authors.affiliation: Centre de Bioinformatique Bordeaux, Université V. Segalen Bordeaux 2, rue Léo Saignat, 33076 Bordeaux Cedex, France.
authors.forename: Aurélien
authors.initials: A
authors.lastname: Barré
authors.affiliation: INRA, UMR 1202 BIOGECO, F-33610 Cestas, France Université de Bordeaux, BIOGECO, UMR 1202, F-33170 Talence, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Plomion
authors.affiliation: Instituto de Biología Vegetal y Biotecnología, Universidad de Talca, 2 Norte 685, Talca, Chile raherre@utalca.cl.
authors.forename: Raul
authors.initials: R
authors.lastname: Herrera
mesh_terms: D016923:Cell Death; D002473:Cell Wall / Q000378:metabolism; D016000:Cluster Analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D028223:Pinus / Q000254:growth & development / Q000378:metabolism*; D010940:Plant Proteins / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D020543:Proteome; D040901:Proteomics; D053719:Tandem Mass Spectrometry; D014197:Trees; D014934:Wood / Q000254:growth & development / Q000378:metabolism; D052584:Xylem / Q000254:growth & development / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D020543:Proteome
keywords: Pinus pinaster Ait.; cambial age; juvenile wood; mature wood; proteome; wood formation