title: Polycarboxylated microfillers incorporated into light-curable resin-based dental adhesives evoke remineralization at the mineral-depleted dentin.
abstract: This study aimed at evaluating the remineralizing properties of three experimental light-curable resin-based dental adhesives containing tailored polycarboxylated microfillers. A co-monomers blend was firstly formulated and then mixed with each of the following microfillers: polycarboxylated bioactive glass (PBAG), polycarboxylated calcium silicates (PCS), and polycarboxylated calcium silicates-doped brushite (PDP). The three experimental and a filler-free control resins were applied onto 10% orthophosphoric acid treated dentin discs and light cured. The specimens were soaked in artificial saliva (AS) for 3, 7, and 14 days. Dentin mineral variation was monitored using attenuated total reflection-Fourier transform infrared (ATR-FTIR) and Raman spectroscopy. Confocal laser scanning microscopy (CLSM) was employed to observe the ultra-morphology/nanoleakage along the resin-dentin interface. The bonding ability and the durability of the resin-dentin bonds were investigated through microtensile bond strength (μTBS) test. ATR-FTIR and Raman showed a significant increase of the mineral matrix area ratio and phosphate peak intensity in specimens treated with the experimental resins within 14 days (p < 0.05). No significant increment of minerals was found in untreated specimens or specimens treated using the control filler-free resin (p > 0.05). Dentin treated using PBAG or PCS exhibited higher level of remineralization than the specimens in PDP group. CLSM showed reduction in nanoleakage, although the remineralization of the hybrid layer induced a significant drop in the μTBS after 3-month storage (p < 0.05). The experimental resin-based dental adhesives containing bioactive microfillers remineralize the resin-dentin interfaces when in intimate contact with biological fluids.
authors.affiliation: a The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University , 237 Luoyu Road, Wuhan , PR China.
authors.forename: Zhejun
authors.initials: Z
authors.lastname: Wang
authors.forename: Ya
authors.initials: Y
authors.lastname: Shen
authors.forename: Markus
authors.initials: M
authors.lastname: Haapasalo
authors.forename: Jiao
authors.initials: J
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.forename: Yining
authors.initials: Y
authors.lastname: Wang
authors.forename: Timothy F
authors.initials: TF
authors.lastname: Watson
authors.forename: Salvatore
authors.initials: S
authors.lastname: Sauro
mesh_terms: D002130:Calcium Phosphates / Q000737:chemistry; D003804:Dentin / Q000737:chemistry*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D006801:Humans; D055115:Light-Curing of Dental Adhesives; D018613:Microscopy, Confocal; D010710:Phosphates / Q000378:metabolism; D011076:Polycarboxylate Cement / Q000737:chemistry; D012117:Resins, Synthetic / Q000737:chemistry*; D012464:Saliva, Artificial / Q000737:chemistry; D012823:Silicate Cement / Q000737:chemistry / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman; D013997:Time Factors; D014089:Tooth Remineralization
chemical_list: D002130:Calcium Phosphates; D017220:Dentin-Bonding Agents; D010710:Phosphates; D011076:Polycarboxylate Cement; D012117:Resins, Synthetic; D012464:Saliva, Artificial; D012823:Silicate Cement; C020243:calcium phosphate