title: Purification and characterization of a β-glucosidase from aspergillus niger and its application in the hydrolysis of geniposide to genipin.
abstract: An extracellular β-glucosidase from Aspergillus niger Au0847 was purified to homogeneity by precipitation with ammonium sulfate, anion exchange, and gel filtration. The purified protein was composed of two subunits with molecular masses of 110 and 120 kDa. Au0847 β-glucosidase exhibited relatively high thermostability and pH stability, and its highest activity was obtained at 65°C and pH 4.6, respectively. As a potential metalloprotein, its enzymatic activity was potently stimulated by manganese ion and DTT. The β-glucosidase displayed avid affinity and high catalytic efficiency for geniposide. Au0847 β-glucosidase has potential value as an industrial enzyme for the hydrolysis of geniposide to genipin.
authors.affiliation: Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Hefei 230031, P. R. China.
authors.forename: Guohong
authors.initials: G
authors.lastname: Gong
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Zheng
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Jinshan
authors.initials: J
authors.lastname: Diao
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Genhai
authors.initials: G
authors.lastname: Zhao
mesh_terms: D001234:Aspergillus niger / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D001711:Biotransformation; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D005959:Glucosidases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D039823:Iridoids / Q000737:chemistry*; D007700:Kinetics
chemical_list: D005656:Fungal Proteins; D039823:Iridoids; C007835:geniposide; C007834:genipin; D005959:Glucosidases