title: Antigen-specific mRNA transfection of autologous dendritic cells.
abstract: Several reports have described the use of tumor-extracted RNA as source of tumor antigen for the preparation of vaccines based on dendritic cells (DC) and its potential use for antigen-specific or polyvalent tumor vaccination. Upon transfection, RNA is transcribed into proteins that enter the cytoplasmic degradation pathway and can be presented by DC through class I major histocompatibility complex (MHC)-I, thus inducing specific T cell cytotoxic responses. In this chapter, we present a protocol to transfect murine dendritic cells with tumor mRNA by means of electroporation.
authors.affiliation: Biomedical Engineering Program, Russ College of Engineering and Technology, Ohio University, Athens, OH, USA.
authors.forename: Fabian
authors.initials: F
authors.lastname: Benencia
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics*; D001854:Bone Marrow Cells / Q000166:cytology; D019496:Cancer Vaccines / Q000235:genetics; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D018274:Electroporation; D005269:Femur / Q000166:cytology; D005434:Flow Cytometry; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013977:Tibia / Q000166:cytology; D014162:Transfection / Q000379:methods*
chemical_list: D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D012333:RNA, Messenger