title: Microsatellite markers to determine population genetic structure in the golden anchovy, Coilia dussumieri.
abstract: Coilia dussumieri (Valenciennes, 1848) commonly called as golden anchovy, constitutes a considerable fishery in the northern part of both the west and east coasts of India. Despite its clear-cut geographic isolation, the species is treated as a unit stock for fishery management purposes. We evaluated 32 microsatellite primer pairs from three closely related species (resource species) belonging to the family Engraulidae through cross-species amplification in C. dussumieri. Successful cross-priming was obtained with 10 loci, which were sequenced for confirmation of repeats. Loci were tested for delineating the genetic stock structure of four populations of C. dussumieri from both the coasts of India. The number of alleles per locus ranged from 8 to 18, with a mean of 12.3. Results of pairwise F ST indicated genetic stock structuring between the east and west coast populations of India and also validated the utilization of identified microsatellite markers in population genetic structure analysis.
authors.affiliation: National Bureau of Fish Genetic Resources Kochi Unit, Cochin, 682 018, Kerala, India, kathirars@gmail.com.
authors.forename: A
authors.initials: A
authors.lastname: Kathirvelpandian
authors.forename: A
authors.initials: A
authors.lastname: Gopalakrishnan
authors.forename: W S
authors.initials: WS
authors.lastname: Lakra
authors.forename: Gopal
authors.initials: G
authors.lastname: Krishna
authors.forename: Rupam
authors.initials: R
authors.lastname: Sharma
authors.forename: K K
authors.initials: KK
authors.lastname: Musammilu
authors.forename: V S
authors.initials: VS
authors.lastname: Basheer
authors.forename: J K
authors.initials: JK
authors.lastname: Jena
mesh_terms: D000818:Animals; D005399:Fishes / Q000235:genetics*; D056426:Genetic Loci; D005828:Genetics, Population; D007194:India; D018895:Microsatellite Repeats; D017422:Sequence Analysis, DNA