title: Effects of angiopoietin-1 on inflammatory injury in endothelial progenitor cells and blood vessels.
abstract: Endothelial progenitor cells (EPCs) and angiopoietin-1 (Ang-1) play important roles in vasculogenesis and angiogenesis, respectively. Thus, targeting both aspects of cardiovascular tissue regeneration may offer promising therapeutic options for cardiovascular disorders. To this end, we constructed a lentiviral vector (pNL) with the Ang-1 gene and transfected EPCs with it (Ang-1-EPCs) to investigate vasculogenesis in both cellular and animal models. Compared to controls, intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) increased significantly in both untreated EPCs and in the pNL vector group. After Ang-1 transcription, ICAM-1 and VCAM-1 decreased considerably in those treatment groups. Ang-1-modified EPCs alleviated inflammatory responses induced by tumor-necrosis factor-α (TNF-α) in vitro. Moreover, Ang-1-EPC implantation inhibited neointimal hyperplasia after balloon catheter injury in rats, dramatically diminishing the intimal-media (I/M) ratio and decreasing the neointimal area. Proliferating cell nuclear antigen expression in the Ang-1-EPC group was lower than the EPC non-treatment group as well, suggesting that Ang-1-EPC improved cell survival during inflammation and promoted endothelialization in damaged blood vessels.
authors.forename: Yi-Qing
authors.initials: YQ
authors.lastname: Wang
authors.forename: Jing-Jin
authors.initials: JJ
authors.lastname: Song
authors.forename: Xiao
authors.initials: X
authors.lastname: Han
authors.forename: Yi-Ye
authors.initials: YY
authors.lastname: Liu
authors.forename: Xi-Huang
authors.initials: XH
authors.lastname: Wang
authors.forename: Zhi-Ming
authors.initials: ZM
authors.lastname: Li
authors.affiliation: Department of Cardiology, Zhongshan Hospital of Xiamen University, Xiamen, Fujian, 361004, China. wang_gina@163.com.
authors.forename: Chi-Meng
authors.initials: CM
authors.lastname: Tzeng
mesh_terms: D042683:Angiopoietin-1 / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D001808:Blood Vessels / Q000378:metabolism* / Q000821:virology; D002470:Cell Survival / Q000235:genetics; D002478:Cells, Cultured; D066026:Endothelial Progenitor Cells / Q000378:metabolism* / Q000821:virology; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics; D007249:Inflammation / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D016086:Lentivirus / Q000235:genetics; D008297:Male; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000378:metabolism / Q000821:virology; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014158:Transcription, Genetic / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism
chemical_list: D042683:Angiopoietin-1; D018809:Proliferating Cell Nuclear Antigen; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1