title: Analysis of protein changes using two-dimensional difference gel electrophoresis.
abstract: A protocol for protein analysis using two-dimensional difference gel electrophoresis (2D-DIGE) is described. 2D-DIGE is one of the most popular and versatile methods of protein separation among rapidly increasing proteomics technologies. Similar to traditional two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), the proteins are separated based on their charges and molecular weight by 2D-DIGE. Different from 2D-PAGE, proteins are pre-labeled with different fluorescent and different protein samples are run in one gel by this method. Therefore, 2D-DIGE not only carries the advantages of 2D-PAGE but also eliminates gel-to-gel variation and achieves high resolution, sensitivity, and reproducibility.
authors.affiliation: Department of Environmental Toxicology, The Institute of Environmental and Human Health (TIEHH), Texas Tech University, Reese Technology Center Building 555, 1207 Gilbert Drive, Lubbock, TX, 79416, USA, weimin.gao@TTU.edu.
authors.forename: Weimin
authors.initials: W
authors.lastname: Gao
mesh_terms: D002478:Cells, Cultured; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D061848:Optical Imaging; D020543:Proteome / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D040901:Proteomics; D057825:Two-Dimensional Difference Gel Electrophoresis
chemical_list: D005456:Fluorescent Dyes; D020543:Proteome