title: [Bactericidal action of non-thermal plasma on biofilms formed in vitro and within a root channel].
abstract: OBJECTIVE
  Evaluation of efficiency of non-thermal plasma as bactericidal agent affecting biofilms formed in vitro and on walls of a root channel.
  
  
  METHODS
  The multiple antibiotic resistant strain Staphylococcus epidermidis isolated from pulpitis was used. Biofilms formed in vitro on the plastic surface and ex vivo at the walls of the root canal were treated with plasma torch formed by argon:air (9:1) mixture eradiated with 100 kHz electrtomagnetic field. Bacterial viability was determined by plating and by differential Live/Dead labeling.
  
  
  RESULTS
  The dose-dependent decrease in living bacteria was demonstrated. The three-step kinetics ofbacterial killing was observed. Total elimination ofup 10(9) CFU/sample was reached at exposition of 240 s or more.
  
  
  CONCLUSIONS
  The non-thermal plasma effectively destroyed bacterial biofilms within root channels.
authors.forename: E V
authors.initials: EV
authors.lastname: Sysoliatina
authors.forename: K A
authors.initials: KA
authors.lastname: Sobianin
authors.forename: N I
authors.initials: NI
authors.lastname: Trushkin
authors.forename: E N
authors.initials: EN
authors.lastname: Beketova
authors.forename: O Iu
authors.initials: OIu
authors.lastname: Arseniukova
authors.forename: T I
authors.initials: TI
authors.lastname: Karpova
authors.forename: A L
authors.initials: AL
authors.lastname: Gintsburg
authors.forename: Iu S
authors.initials: IuS
authors.lastname: Akishev
authors.forename: S A
authors.initials: SA
authors.lastname: Ermolaeva
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001128:Argon / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D003786:Dental Pulp Cavity / Q000382:microbiology*; D004574:Electromagnetic Fields; D006801:Humans; D013212:Staphylococcus epidermidis / Q000502:physiology*
chemical_list: D000900:Anti-Bacterial Agents; D001128:Argon