title: Vaccine-associated Leptospira antibodies in client-owned dogs.
abstract: BACKGROUND
  Long-term microscopic agglutination test (MAT) results after vaccination with 4-serovar Leptospira vaccines are not available for all vaccines used in client-owned dogs.
  
  
  OBJECTIVE
  To determine antibody responses of client-owned dogs given 1 of 4 commercially available Leptospira vaccines.
  
  
  METHODS
  Healthy client-owned dogs (n = 32) with no history of Leptospira vaccination for at least the previous year.
  
  
  METHODS
  Dogs were given 1 of 4 Leptospira vaccines on week 0 and then approximately on week 3 and week 52. Sera were collected before vaccine administration on week 0 and then within 3 days of week 3, within 2 days of week 4, and approximately on weeks 7, 15, 29, 52, and 56. Antibody titers against Leptospira serovars bratislava, canicola, grippotyphosa, hardjo, icterohemorrhagiae, and pomona and were determined by MAT.
  
  
  RESULTS
  When compared among vaccines, MAT results varied in maximal titers, the serovars inducing maximal titers, and the time required to reach maximal titers. Each vaccine induced at least some MAT titers ≥1 : 800. Most dogs were negative for antibodies against all serovars 1 year after vaccination, and anamnestic responses were variable.
  
  
  CONCLUSIONS
  Dogs vaccinated with Leptospira vaccines have variable MAT titers over time, and antibodies should not be used to predict resistance to Leptospira infection. MAT titers ≥1 : 800 can develop after Leptospira spp. vaccination, which can complicate the clinical diagnosis of leptospirosis.
authors.affiliation: Department of Clinical Sciences, Colorado State University, Fort Collins, CO.
authors.forename: L E R
authors.initials: LE
authors.lastname: Martin
authors.forename: K T
authors.initials: KT
authors.lastname: Wiggans
authors.forename: S A
authors.initials: SA
authors.lastname: Wennogle
authors.forename: K
authors.initials: K
authors.lastname: Curtis
authors.forename: R
authors.initials: R
authors.lastname: Chandrashekar
authors.forename: M R
authors.initials: MR
authors.lastname: Lappin
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D004283:Dog Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control; D004285:Dogs / Q000276:immunology* / Q000382:microbiology; D007919:Leptospira / Q000276:immunology*; D007922:Leptospirosis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines
keywords: Serology; Titers