title: Early tissue responses in psoriasis to the antitumour necrosis factor-α biologic etanercept suggest reduced interleukin-17 receptor expression and signalling.
abstract: BACKGROUND
  Antitumour necrosis factor (anti-TNF)-α therapy has made a significant impact on the treatment of psoriasis. Despite these agents being designed to neutralize TNF-α activity, their mechanism of action in the resolution of psoriasis remains unclear.
  
  
  OBJECTIVE
  To understand better the mechanism of action of etanercept by examining very early changes in the lesional skin of patients with psoriasis responding to etanercept.
  
  
  METHODS
  Twenty patients with chronic plaque psoriasis were enrolled and received etanercept 50 mg twice weekly. Skin biopsies were obtained before treatment and on days 1, 3, 7 and 14 post-treatment. Skin mRNA expression was analysed by quantitative reverse-transcription polymerase chain reaction and microarray; cytokine and phosphoprotein levels were assessed using multiplexed bead arrays.
  
  
  RESULTS
  In etanercept responders, we observed no significant changes in interleukin (IL)-17A, IL-22 or interferon-γ mRNA or protein in the first week of treatment; however, there was a 2·5-fold downregulation of IL-17 receptor C (IL-17RC) mRNA (P < 0·05) after day 1, accompanied by decreased extracellular signal-regulated kinase-1/2 phosphorylation. Transcriptional analysis revealed that genes suppressed by etanercept significantly overlapped with IL-17A-induced genes, and a marked overlap was also observed between the genes suppressed by etanercept and by the anti-IL-17A agent ixekizumab. Finally we show that TNF-α enhances the expression of IL-17RC, and short hairpin RNA inhibition of IL-17R expression abrogates synergistic gene induction by TNF and IL-17A.
  
  
  CONCLUSIONS
  These results suggest that the early responses of psoriasis plaques to etanercept may be due to decreased tissue responsiveness to IL-17A due to suppressed IL-17RC expression in keratinocytes, blunting the strong synergy between TNF-α and IL-17, which contributes to the maintenance of psoriasis lesions.
authors.affiliation: Department of Dermatology, University of Michigan, Ann Arbor, MI, 48109, U.S.A.
authors.forename: A
authors.initials: A
authors.lastname: Johnston
authors.forename: A M
authors.initials: AM
authors.lastname: Guzman
authors.forename: W R
authors.initials: WR
authors.lastname: Swindell
authors.forename: F
authors.initials: F
authors.lastname: Wang
authors.forename: S
authors.initials: S
authors.lastname: Kang
authors.forename: J E
authors.initials: JE
authors.lastname: Gudjonsson
mesh_terms: D000293:Adolescent; D000328:Adult; D000963:Antimetabolites / Q000494:pharmacology; D001688:Biological Products / Q000627:therapeutic use; D002908:Chronic Disease; D003879:Dermatologic Agents / Q000627:therapeutic use*; D000068800:Etanercept; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000627:therapeutic use*; D015603:Keratinocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011565:Psoriasis / Q000188:drug therapy*; D012333:RNA, Messenger / Q000378:metabolism; D053722:Receptors, Interleukin-17 / Q000187:drug effects / Q000378:metabolism*; D018124:Receptors, Tumor Necrosis Factor / Q000627:therapeutic use*; D015398:Signal Transduction / Q000187:drug effects; D046888:Tissue Array Analysis; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D055815:Young Adult
chemical_list: D000963:Antimetabolites; D001688:Biological Products; D003879:Dermatologic Agents; D007074:Immunoglobulin G; D012333:RNA, Messenger; D053722:Receptors, Interleukin-17; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha; D000068800:Etanercept