title: Purification of G1 daughter cells from different Saccharomycetes species through an optimized centrifugal elutriation procedure.
abstract: Centrifugal elutriation discriminates cells according to their sedimentation coefficients, generating homogeneous samples well suited for genomic comparative approaches. It can, for instance, isolate G1 daughter cells from a Saccharomyces cerevisiae unsynchronized population, alleviating ageing and cell-cycle biases when conducting genome-wide/single-cell studies. The present report describes a straightforward and robust procedure to determine whether a cell population of virtually any yeast species can be efficiently elutriated, while offering solutions to optimize success. This approach was used to characterize elutriation parameters and S-phase progression of four yeast species (S. cerevisiae, Candida glabrata, Lachancea kluyveri and Pichia sorbitophila) and could theoretically be applied to any culture of single, individual cells.
authors.affiliation: Institut Pasteur, Group Spatial Regulation of Genomes, Department of Genomes and Genetics, F-75015, Paris, France; CNRS, UMR3525, F-75015, Paris, France.
authors.forename: Martial
authors.initials: M
authors.lastname: Marbouty
authors.forename: Caroline
authors.initials: C
authors.lastname: Ermont
authors.forename: Bernard
authors.initials: B
authors.lastname: Dujon
authors.forename: Guy-Franck
authors.initials: GF
authors.lastname: Richard
authors.forename: Romain
authors.initials: R
authors.lastname: Koszul
mesh_terms: D002455:Cell Division; D002498:Centrifugation / Q000379:methods*; D016193:G1 Phase; D009172:Mycology / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics / Q000302:isolation & purification; D015003:Yeasts / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification
keywords: Ascomyceta; daughter cells; elutriation; replication; synchronization; yeast