title: Characterization of a novel citrus tristeza virus genotype within three cross-protecting source GFMS12 sub-isolates in South Africa by means of Illumina sequencing.
abstract: Tristeza disease (caused by citrus tristeza virus, CTV) is currently controlled in South Africa by means of cross-protection. In this study, we characterized the CTV populations of three grapefruit mild strain 12 (GFMS12) single-aphid-transmission-derived sub-isolates at the whole-genome level using Illumina sequencing technology. A novel South African isolate (CT-ZA3, of the T68 genotype) was shown to be the dominant genotype in all GFMS12 sub-isolates tested, along with reads unique to various other genotypes occurring as minor components. Uncertainty remains as to the significance of these minor components.
authors.affiliation: Department of Microbiology and Plant Pathology and FABI, University of Pretoria, New Agricultural Building, Pretoria, 0002, South Africa, Olivier.zablocki@fabi.up.ac.za.
authors.forename: Olivier
authors.initials: O
authors.lastname: Zablocki
authors.forename: Gerhard
authors.initials: G
authors.lastname: Pietersen
mesh_terms: D000818:Animals; D001042:Aphids / Q000502:physiology; D032083:Citrus paradisi / Q000276:immunology / Q000821:virology*; D017790:Closterovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D056738:Cross Protection; D016679:Genome, Viral; D005838:Genotype; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000276:immunology / Q000821:virology*; D017422:Sequence Analysis, DNA; D013019:South Africa