title: Imprecision and limit of quantification of the new architect 25-hydroxyvitamin D assay.
abstract: We examined imprecision and limit of quantification (LOQ) of the new Architect 25-hydroxyvitamin D immunoassay in a multicenter setting. Mean intracenter interlot imprecision varied from 22.0 to 5.0%CV in the concentration range of 16 - 92 nmol/L. LOQs were locally determined as 10.8, 11.2, and 13.5 nmol/L. We conclude that the Architect 25(OH)D assay provides a precise and analytically sensitive method for routine measurement of 25(OH)D.
authors.affiliation: VieCuri Medical Center, Laboratory of Clinical Chemistry and Hematology, Venlo, The Netherlands. marceljanssen@viecuri.nl
authors.forename: Marcel J W
authors.initials: MJ
authors.lastname: Janssen
authors.affiliation: Reinier de Graaf Gasthuis, Department of Clinical Chemistry, Delft, The Netherlands.
authors.forename: Frans A L
authors.initials: FA
authors.lastname: Van der Horst
authors.affiliation: Gelre Hospitals, Department of Clinical Chemistry and Hematology, Apeldoorn, The Netherlands.
authors.forename: Johannes S
authors.initials: JS
authors.lastname: Kamphuis
mesh_terms: D006801:Humans; D057230:Limit of Detection; D015203:Reproducibility of Results; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000097:blood
chemical_list: D014807:Vitamin D; C104450:25-hydroxyvitamin D