title: Promoter polymorphisms of ST3GAL4 and ST6GAL1 genes and associations with risk of premalignant and malignant lesions of the cervix.
abstract: Sialyltransferase gene expression is altered in several cancers, including examples in the cervix. Transcriptional regulation of the responsible genes depends on different promoters. We aimed to determine the association of single-nucleotide polymorphisms in the B3 promoter of the ST3GAL4 gene and the P1 promoter of the ST6GAL1 gene with cervical premalignant lesions or cervical cancer. A blood sample and/or cervical scrapes were obtained from 104 women with normal cytology, 154 with premalignant lesions and 100 with cervical cancer. We also included 119 blood samples of random donors. The polymorphisms were identified by sequencing from PCR products. For the B3 promoter, a fragment of 506 bp (from nucleotide -408 to +98) was analyzed, and for the P1 promoter a 490 bp (-326 to +164) fragment. The polymorphism analysis showed that at SNP rs10893506, genotypes CC and CT of the ST3GAL4 B3 promoter were associated with the presence of premalignant lesions (OR=2.89; 95%CI 1.72-4.85) and cervical cancer (OR=2.23; 95%CI 1.27-3.91). We detected only one allele of each polymorphism in the ST6GAL1 P1 promoter. We did not detect any genetic variability in the P1 promoter region in our study population. Our results suggest that the rs10893506 polymorphism -22C/T may increase susceptibility to premalignant and malignant lesions of the cervix.
authors.affiliation: Laboratorio de Biologia Molecular y Virologia, Centro de Investigacion Biomedica de Oriente, Instituto Mexicano del Seguro Social, BUAP, Mexico E-mail : veronica_vallejo@yahoo.com.
authors.forename: Maria de Los Angeles
authors.initials: Mde L
authors.lastname: Rivera-Juarez
authors.forename: Nora Hilda
authors.initials: NH
authors.lastname: Rosas-Murrieta
authors.forename: Victoriano
authors.initials: V
authors.lastname: Mendieta-Carmona
authors.forename: Raquel Esneidy
authors.initials: RE
authors.lastname: Hernandez-Pacheco
authors.forename: Irma
authors.initials: I
authors.lastname: Zamora-Ginez
authors.forename: Carlos
authors.initials: C
authors.lastname: Rodea-Avila
authors.forename: Teresa
authors.initials: T
authors.lastname: Apresa-Garcia
authors.forename: Onix
authors.initials: O
authors.lastname: Garay-Villar
authors.forename: Adriana
authors.initials: A
authors.lastname: Aguilar-Lemarroy
authors.forename: Luis Felipe
authors.initials: LF
authors.lastname: Jave-Suarez
authors.forename: Maria Alicia
authors.initials: MA
authors.lastname: Diaz-Orea
authors.forename: Lorena
authors.initials: L
authors.lastname: Milflores-Flores
authors.forename: Juan Salvador
authors.initials: JS
authors.lastname: Reyes-Salinas
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Ceja-Utrera
authors.forename: Victor Javier
authors.initials: VJ
authors.lastname: Vazquez-Zamora
authors.forename: Tomas
authors.initials: T
authors.lastname: Vargas-Maldonado
authors.forename: Sandra
authors.initials: S
authors.lastname: Reyes-Carmona
authors.forename: Francisca
authors.initials: F
authors.lastname: Sosa-Jurado
authors.forename: Gerardo
authors.initials: G
authors.lastname: Santos-Lopez
authors.forename: Julio
authors.initials: J
authors.lastname: Reyes-Leyva
authors.forename: Veronica
authors.initials: V
authors.lastname: Vallejo-Ruiz
mesh_terms: D015703:Antigens, CD / Q000097:blood / Q000235:genetics*; D001483:Base Sequence; D002584:Cervix Uteri / Q000473:pathology*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D011230:Precancerous Conditions / Q000235:genetics* / Q000473:pathology; D011401:Promoter Regions, Genetic; D020033:Protein Isoforms / Q000235:genetics; D017422:Sequence Analysis, DNA; D012799:Sialyltransferases / Q000097:blood / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000097:blood / Q000235:genetics*; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
chemical_list: D015703:Antigens, CD; D020033:Protein Isoforms; D012799:Sialyltransferases; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase; C093493:ST6GAL1 protein, human; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase; C000722087:ST3GAL1 protein, human