title: LC-MS-based holistic metabolic profiling. Problems, limitations, advantages, and future perspectives.
abstract: The present review aims to critically discuss some of the major problems and limitations of LC-MS based metabolomics as experienced from an analytical chemistry standpoint. Metabolomics offers distinct advantages to a variety of life sciences. Continuous development of the field has been realised due to intensive efforts from a great many scientists from widely divergent backgrounds and research interests as demonstrated by the contents of this special issue. The aim of this commentary is to describe current hindrances to field's progress, (some unique to metabolomics, some common with other omics fields or with conventional targeted bioanalysis) to propose some potential solutions to overcome these constraints and to provide a future perspective for likely developments in the field.
authors.affiliation: Department of Chemical Engineering, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece. Electronic address: gkikae@auth.gr.
authors.forename: Helen G
authors.initials: HG
authors.lastname: Gika
authors.affiliation: Department of Surgery and Cancer, Faculty of Medicine, Imperial College, London, UK.
authors.forename: Ian D
authors.initials: ID
authors.lastname: Wilson
authors.affiliation: Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
authors.forename: Georgios A
authors.initials: GA
authors.lastname: Theodoridis
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods* / Q000592:standards / Q000639:trends; D019295:Computational Biology; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods* / Q000592:standards / Q000639:trends; D055432:Metabolomics / Q000379:methods* / Q000592:standards / Q000639:trends; D011786:Quality Control
keywords: Mass spectrometry; Metabolomics; Metabonomics; Quality control; Standardisation