title: Antioxidant and hepatoprotective effects of naringenin and its β-cyclodextrin formulation in mice intoxicated with carbon tetrachloride: a comparative study.
abstract: The present study evaluated the antioxidant and hepatoprotective effects of the flavonoid naringenin (NGN) and its β-cyclodextrin formulation at a dose of 50 mg/kg b.w. The assessment was done by the investigation of serum-enzymatic and liver antioxidant activity, histopathological and ultrastructural changes in male Swiss mice, which were subjected to acute experimental intoxication with CCl4. Formulated and free flavonoid were orally given to mice for 7 days and then were intraperitoneally injected with 1.0 mL/kg CCl4 on the 8th day. After 24 h of CCl4 administration, an increase in the levels of transaminases aspartate aminotransferase and alanine aminotransferase activities and malondialdehyde concentration occurred and a significant decrease in superoxide dismutase, catalase glutathione-peroxidase activities, and glutathione levels was detected as well. These were accompanied by extended centrilobular necrosis, steatosis, fibrosis, and an altered ultrastructure of hepatocytes. Pretreatment with formulated or free flavonoid retained the biochemical markers to control values. Histopathological and electron-microscopic examination confirmed the biochemical results. In conclusion, both NGN and NGN/β-cyclodextrin complex showed antioxidant and hepatoprotective effects against injuries induced by CCl4.
authors.affiliation: 1 Department of Histology, Faculty of Medicine, Pharmacy and Dentistry, Vasile Goldis Western University of Arad , Arad, Romania .
authors.forename: Anca
authors.initials: A
authors.lastname: Hermenean
authors.forename: Aurel
authors.initials: A
authors.lastname: Ardelean
authors.forename: Miruna
authors.initials: M
authors.lastname: Stan
authors.forename: Nicoleta
authors.initials: N
authors.lastname: Hadaruga
authors.forename: Ciprian-Valentin
authors.initials: CV
authors.lastname: Mihali
authors.forename: Marieta
authors.initials: M
authors.lastname: Costache
authors.forename: Anca
authors.initials: A
authors.lastname: Dinischiotu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D002099:Cacao / Q000737:chemistry; D002252:Carbon Tetrachloride Poisoning / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D002957:Citrus / Q000737:chemistry; D016503:Drug Delivery Systems; D044950:Flavanones / Q000494:pharmacology / Q000627:therapeutic use*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D018551:Solanum lycopersicum / Q000737:chemistry; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D047392:beta-Cyclodextrins
chemical_list: D000975:Antioxidants; D044950:Flavanones; D010936:Plant Extracts; D047392:beta-Cyclodextrins; D013482:Superoxide Dismutase; C005273:naringenin; C031215:betadex
keywords: CCl4; antioxidant activity; hepatoprotection; naringenin