title: Deficiency of MTMR14 promotes autophagy and proliferation of mouse embryonic fibroblasts.
abstract: MTMR14 is a phosphoinositide phosphatase, which has been reported to regulate the maintenance of normal muscle performance and aging in mice. However, the function of MTMR14 in mouse embryonic fibroblasts (MEFs) remains largely unknown. In this study, we established MTMR14 WT and KO MEFs and showed that MTMR14 is localized in whole MEFs, with higher level in nucleus and lower in cytoplasm, partially overlapping with mitochondrial. Compared with the WT control, MTMR14 KO MEFs exhibit a higher proliferation rate and more obvious autophagy. Furthermore, we demonstrate that KO of MTMR14 significantly decreased the mRNA levels of p21 and p27, while increased those of cyclinD and cyclinE. Upon (insulin-like growth factor) IGF stimulation, we also found KO of MTMR14 enhanced the phosphorylation levels of AKT and ERK in MEFs. Based on these findings, we propose that defect of MTMR14 promotes autophagy and cell proliferation in MEFs.
authors.affiliation: Institute For Medical Biology and Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Sciences, South-Central University for Nationalities, Wuhan, 430074, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Yin
authors.initials: Y
authors.lastname: Lv
authors.forename: Qing-hua
authors.initials: QH
authors.lastname: Liu
authors.forename: Cheng-Kui
authors.initials: CK
authors.lastname: Qu
authors.forename: Jinhua
authors.initials: J
authors.lastname: Shen
mesh_terms: D000818:Animals; D001343:Autophagy / Q000235:genetics*; D001483:Base Sequence; D049109:Cell Proliferation / Q000235:genetics*; D002478:Cells, Cultured; D017931:DNA Primers; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics / Q000502:physiology*; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; C554751:MTMR14 protein, mouse; D010744:Phosphoric Monoester Hydrolases