title: Recent achievments in the design and engineering of artificial metalloenzymes.
abstract: Herein, we highlight a selection of recent successes in the creation of artificial metalloenzymes. A particular emphasis is set on different anchoring methods to incorporate the abiotic metal cofactor within the host protein as well as promising strategies for the de novo design of artificial metalloenzymes. Both approaches yield promiscuous catalytic activities which expand the catalytic repertoire of biocatalysis and synthetic biology. Moreover, we summarize laboratory evolution protocols which have contributed to unravel the full potential of artificial metalloenzymes.
authors.affiliation: Departement of Chemistry, University of Basel, Spitalstrasse 51, Basel CH-4056, Switzerland.
authors.forename: Marc
authors.initials: M
authors.lastname: Dürrenberger
authors.affiliation: Departement of Chemistry, University of Basel, Spitalstrasse 51, Basel CH-4056, Switzerland. Electronic address: Thomas.Ward@unibas.ch.
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Ward
mesh_terms: D055162:Biocatalysis; D004247:DNA / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D008667:Metalloproteins / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction; D015202:Protein Engineering
chemical_list: D008667:Metalloproteins; D004247:DNA