title: Correlation of sperm parameters with semen lipid peroxidation and total antioxidants levels in astheno- and oligoasheno- teratospermic men.
abstract: BACKGROUND
  Sperm dysfunction caused by reactive oxygen species (ROSs) is one of the major causes of infertility in men, which leads to, lipid peroxidation (LPO) and the formation of stable peroxidation products like Malondialdehyde (MDA) in seminal plasma. MDA is effective factor in reducing fertility.
  
  
  OBJECTIVE
  The aim of this study is to determine two biochemical markers of oxidative stress; TAC and MDA, and them correlation to quality-quantity factors in Asthenoteratospermic and Oligoashenoteratospermic men.
  
  
  METHODS
  A total of 42 semen samples including: 15 samples normospermic as control group, 12 Asthenoteratospermic and 15 oligoasthenoteratospermic were collected from Babol IVF center; Iran. Semen analysis was performed according to WHO (1999) guidelines. Seminal plasma TAC and MDA levels in all patients were measured by TBARs and FRAP methods, respectively.
  
  
  RESULTS
  Seminal plasma TAC level in normospermic men was significantly higher than asthenoteratospermic men (P < 0.001) and oligasthenoteratospermic men (P < 0.001) and had posetive correlation with sperm count, motility and morphology. In contrast MDA levels in normospermic men were significantly lower than in asthenoteratospermic men (P = 0.049) and oligoasthenoteratospermic men (P = 0.001) and had negative correlation with sperm count, motility and morphology.
  
  
  CONCLUSIONS
  These results suggest that lipid peroxidation and decreasing total antioxidant capacity lead to low motility; morphology and sperm count in spermatozoa of astheno-and oligoastheno-teratospermic men. Therefore, evaluation of oxidative status and antioxidant defenses system may be as a useful tool for diagnosis and treatment of male infertility especially in idiopathic male infertility.
authors.affiliation: Department of Molecular and Cell Biology; Nano and Biotechnology Research Group, Faculty of Basic Sciences, University of Mazandaran, Babolsar, IR Iran.
authors.forename: Abasalt
authors.initials: A
authors.lastname: Hosseinzadeh Colagar
authors.affiliation: Department of Molecular and Cell Biology; Nano and Biotechnology Research Group, Faculty of Basic Sciences, University of Mazandaran, Babolsar, IR Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Karimi
authors.affiliation: Fatemeh-Zahra Infertility and Health Reproductive Research Center, Babol University of Medical Sciences, Babol, IR Iran.
authors.forename: Seyed Gholam Ali
authors.initials: SG
authors.lastname: Jorsaraei
keywords: Lipid Peroxidation; Malondialdehyde; Reactive Oxygen Species