title: [Effect of Aloe emodin on invasion and metastasis of high metastatic breast cancer MDA-MB-231 cells].
abstract: OBJECTIVE
  To investigate the effect of Aloe emodin (AE) on the invasive and metastatic abilities of human high metastatic breast cancer MDA-MB-231 cells.
  
  
  METHODS
  MTT assay was used to evaluate the viability of MDA-MB-231 cells after treated with AE for 6 h and 24 h. The adhesive potential of MDA-MB-231 cells to FN and LN was tested by cell-matrix adhesion assay. The effect of AE on invasion of MDA-MB-231 cells was measured by Transwell chamber assay. Scratch wound healing assay was applied to determine the effect on migration of MDA-MB-231 cells. The effect of AE on MDA-MB-231 lung metastasis was determined on an experimental metastatic model.
  
  
  RESULTS
  80 micromol/L AE significantly inhibited the invasion, adhesion to FN, LN of MDA-MB-231 cells in vitro, the inhibitory rates were (52.98 +/- 5.46)%, (34.99 +/- 2.63)%, (28.73 +/- 7.00)%, respectively. After 24 h treatment, AE significantly inhibited the migration of MDA-MB-231 cells. The number and volume of lung metastatic nodules formed by MDA-MB-231 cells after 80 micromol/L AE 24 h treatment were decreased compared with control group.
  
  
  CONCLUSIONS
  AE can suppress the metastasis of MDA-MB-231 cells. Their mechanisms may be related to the inhibition of the capabilities of invasion and migration of MDA-MB-231 cells.
authors.forename: Zhen-Hui
authors.initials: ZH
authors.lastname: He
authors.forename: Yue-Qun
authors.initials: YQ
authors.lastname: Huang
authors.forename: Shan-Fan
authors.initials: SF
authors.lastname: Weng
authors.forename: Yao-Rong
authors.initials: YR
authors.lastname: Tan
authors.forename: Tai-Ping
authors.initials: TP
authors.lastname: He
authors.forename: Yan-Mei
authors.initials: YM
authors.lastname: Qin
authors.forename: Nian-Ci
authors.initials: NC
authors.lastname: Liang
mesh_terms: D000504:Aloe / Q000737:chemistry; D000818:Animals; D000880:Anthraquinones / Q000008:administration & dosage / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology*; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000517:prevention & control / Q000556:secondary; D008807:Mice, Inbred BALB C; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis / Q000517:prevention & control*; D009368:Neoplasm Transplantation; D023041:Xenograft Model Antitumor Assays
chemical_list: D000880:Anthraquinones; D000972:Antineoplastic Agents, Phytogenic; C518327:aloe emodin