title: A purge and trap technique to capture volatile compounds combined with comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry to investigate the effect of sulfur-fumigation on Radix Angelicae Dahuricae.
abstract: Sulfur-fumigation is known to reduce volatile compounds that are the main active components in herbs used in herbal medicine. We investigated changes in chemical composition between sun-dried and sulfur-fumigated Radix Angelicae Dahuricae using a purge and trap technique to capture volatile compounds, and two-dimensional gas chromatography/time-of-flight mass spectrometry for identification. Using sun-dried Radix Angelicae Dahuricae samples as a reference, the results showed that 73 volatile compounds, including 12 sulfide compounds, were found to be present only in sulfur-fumigated samples. Furthermore, 32 volatile compounds that were found in sun-dried Radix Angelicae Dahuricae samples disappeared after sulfur-fumigation. The proposed method can be applied to accurately discriminate sulfur-fumigated Radix Angelicae Dahuricae from different commercial sources.
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, People's Republic of China; Research Center of Traditional Chinese Medicine Processing Technology, Zhejiang Chinese Medical University, Hangzhou, 310053, People's Republic of China.
authors.forename: Gang
authors.initials: G
authors.lastname: Cao
authors.forename: Qinglin
authors.initials: Q
authors.lastname: Li
authors.forename: Jida
authors.initials: J
authors.lastname: Zhang
authors.forename: Hao
authors.initials: H
authors.lastname: Cai
authors.forename: Baochang
authors.initials: B
authors.lastname: Cai
mesh_terms: D029969:Angelica / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005651:Fumigation / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D013455:Sulfur / Q000737:chemistry; D055549:Volatile Organic Compounds / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D004365:Drugs, Chinese Herbal; D055549:Volatile Organic Compounds; D013455:Sulfur
keywords: Radix Angelicae Dahuricae; comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry; dynamic headspace; sulfur-fumigation; volatiles