title: Effects of dietary supplementation with vitamin C and vitamin E and their combination on growth performance, some biochemical parameters, and oxidative stress induced by copper toxicity in broilers.
abstract: This study investigated effects of dietary supplementation with vitamin C, vitamin E on performance, biochemical parameters, and oxidative stress induced by copper toxicity in broilers. A total of 240, 1-day-old, broilers were assigned to eight groups with three replicates of 10 chicks each. The groups were fed on the following diets: control (basal diet), vitamin C (250 mg/kg diet), vitamin E (250 mg/kg diet), vitamin C + vitamin E (250 mg/kg + 250 mg/kg diet), and copper (300 mg/kg diet) alone or in combination with the corresponding vitamins. At the 6th week, the body weights of broilers were decreased in copper, copper + vitamin E, and copper + vitamin C + vitamin E groups compared to control. The feed conversion ratio was poor in copper group. Plasma aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase activities, iron, copper concentrations, and erythrocyte malondialdehyde were increased; plasma vitamin A and C concentrations and erythrocyte superoxide dismutase were decreased in copper group compared to control. Glutathione peroxidase, vitamin C, and iron levels were increased; aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, and copper levels were decreased in copper + vitamin C group, while superoxide dismutase, glutathione peroxidase, and vitamin E concentrations were increased; aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase were decreased in copper with vitamin E group compared to copper group. The vitamin C concentrations were increased; copper, uric acid, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, and malondialdehyde were decreased in copper + vitamin C + vitamin E group compared to copper group. To conclude, copper caused oxidative stress in broilers. The combination of vitamin C and vitamin E addition might alleviate the harmful effects of copper as demonstrated by decreased lipid peroxidation and hepatic enzymes.
authors.affiliation: Department of Biochemistry, Faculty of Veterinary Medicine, Kirikkale University, 71450, Yahsihan/Kirikkale, Turkey, miyase63@hotmail.com.
authors.forename: Miyase
authors.initials: M
authors.lastname: Cinar
authors.forename: Ebru
authors.initials: E
authors.lastname: Yildirim
authors.forename: A Arzu
authors.initials: AA
authors.lastname: Yigit
authors.forename: Ilkay
authors.initials: I
authors.lastname: Yalcinkaya
authors.forename: Ozkan
authors.initials: O
authors.lastname: Duru
authors.forename: Uçler
authors.initials: U
authors.lastname: Kisa
authors.forename: Nurgul
authors.initials: N
authors.lastname: Atmaca
mesh_terms: D000410:Alanine Transaminase / Q000037:antagonists & inhibitors / Q000097:blood / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D001219:Aspartate Aminotransferases / Q000037:antagonists & inhibitors / Q000097:blood / Q000378:metabolism; D002645:Chickens / Q000254:growth & development* / Q000378:metabolism*; D003300:Copper / Q000008:administration & dosage / Q000633:toxicity*; D019587:Dietary Supplements; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D013329:Structure-Activity Relationship; D014810:Vitamin E / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D014810:Vitamin E; D003300:Copper; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D001205:Ascorbic Acid