title: Phyllanthus amarus does not affect hypernociception in experimental autoimmune encephalomyelitis.
abstract: Multiple sclerosis is an inflammatory disease of the central nervous system. Chronic pain is one of the main symptoms, affecting many patients. Studies show that the lignans or the apolar extracts of Phyllanthus amarus have antinociceptive effects in different animal models. To evaluate the antihypernociceptive effect of a hexanic extract of P. amarus in experimental autoimmune encephalomyelitis in mice, the chemical composition of the hexanic extract was analyzed by gas chromatography mass spectrometry. After EAE induction, animals were treated with the hexanic extract of P. amarus for 26 consecutive days. Motor coordination and mechanical hypernociception were evaluated on alternate days. The principal lignans found were phyllanthin, niranthin, and 5-demethoxyniranthin. The hexanic extract of P. amarus at a dose of 100, 200, or 400 mg/kg did not affect the development of the disease. The motor coordination and pain threshold of the treated animals were not altered in this experiment. In conclusion, in this test, the hexanic extract of P. amarus did not show evidence of antihypernociceptive activity in experimental autoimmune encephalomyelitis.
authors.affiliation: Departamento de Psicobiologia, Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Graziella Rigueira
authors.initials: GR
authors.lastname: Molska
authors.affiliation: Departamento de Psicobiologia, Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Negri
authors.affiliation: Departamento de Psicobiologia, Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Lyvia Izaura Gomes
authors.initials: LI
authors.lastname: Paula-Freire
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Leandro Pires
authors.initials: LP
authors.lastname: Araujo
authors.affiliation: Departamento de Psicobiologia, Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Daniele Oliveira
authors.initials: DO
authors.lastname: Köhn
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Alexandre Salgado
authors.initials: AS
authors.lastname: Basso
authors.affiliation: Departamento de Medicina Preventiva, Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Elisaldo Araújo
authors.initials: EA
authors.lastname: Carlini
mesh_terms: D000818:Animals; D000840:Anisoles / Q000032:analysis / Q000494:pharmacology* / Q000627:therapeutic use; D004149:Dioxoles / Q000032:analysis / Q000494:pharmacology* / Q000627:therapeutic use; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000188:drug therapy; D005260:Female; D006930:Hyperalgesia / Q000188:drug therapy / Q000209:etiology; D017705:Lignans / Q000032:analysis / Q000494:pharmacology* / Q000627:therapeutic use; D051379:Mice; D008810:Mice, Inbred C57BL; D009103:Multiple Sclerosis / Q000150:complications* / Q000188:drug therapy; D029803:Phyllanthus / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000840:Anisoles; D004149:Dioxoles; D017705:Lignans; D010936:Plant Extracts; C475932:niranthin; C517985:phyllanthin