title: Fabrication of Au@Ag core-shell NPs as enhanced CT contrast agents with broad antibacterial properties.
abstract: Au@Ag core-shell nanoparticles (NPs) integrating both antibacterial and X-ray attenuation capabilities were facilely synthesized in aqueous solution. These NPs modified with methoxy-PEG-SH (m-PEG) on the surface rendered them favorable dispersity and stability in water, resulting in enhancement of their blood circulation time. X-ray photoelectron spectroscope (XPS) and high-resolution transmission electron microscope (HRTEM) results confirmed the core-shell structure of m-PEG-Au@Ag NPs. The m-PEG-Au@Ag NPs showed low cytotoxicity and strong X-ray absorption potency in vitro. Further in vivo study showed that as-synthesized NPs offered a pronounced contrast and prolonged their circulation time in the blood stream with negligible toxic effect in vivo. Besides, m-PEG-Au@Ag NPs had significant bacteriostatic effect toward common bacteria like Escherichia coli and Staphylococcus aureus as demonstrated by broth dilution assay. Given their low-cytotoxicity and high CT attenuation efficacy, m-PEG-Au@Ag NPs had a promising potential for use as CT enhancing and antibacterial agents.
authors.affiliation: Institute of Materials Engineering, National Laboratory of Solid State Microstructure, College of Engineering and Applied Sciences, Nanjing University, Jiangsu 210093, PR China; Department of Radiology, Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210093, PR China.
authors.forename: Da
authors.initials: D
authors.lastname: Huo
authors.affiliation: Department of Radiology, Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210093, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Radiology, Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210093, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Radiology, Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210093, PR China.
authors.forename: Haiping
authors.initials: H
authors.lastname: Yu
authors.affiliation: Department of Radiology, Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210093, PR China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Shi
authors.affiliation: Jiangsu Entry-Exit Inspection and Quarantine Bureau, Jiangsu 210001, PR China.
authors.forename: Yahui
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Department of Radiology, Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210093, PR China. Electronic address: zyzhou@nju.edu.cn.
authors.forename: Zhengyang
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Institute of Materials Engineering, National Laboratory of Solid State Microstructure, College of Engineering and Applied Sciences, Nanjing University, Jiangsu 210093, PR China. Electronic address: hvyong@nju.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Hu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000008:administration & dosage; D004926:Escherichia coli / Q000187:drug effects; D006046:Gold / Q000494:pharmacology*; D006801:Humans; D053768:Metal Nanoparticles / Q000648:ultrastructure; D051379:Mice; D008826:Microbial Sensitivity Tests; D009928:Organ Specificity / Q000187:drug effects; D056951:Photoelectron Spectroscopy; D011092:Polyethylene Glycols / Q000737:chemistry; D051381:Rats; D012834:Silver / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet; D013211:Staphylococcus aureus / Q000187:drug effects; D014018:Tissue Distribution / Q000187:drug effects; D014057:Tomography, X-Ray Computed
chemical_list: D000900:Anti-Bacterial Agents; D003287:Contrast Media; D012834:Silver; D011092:Polyethylene Glycols; D006046:Gold; C028210:monomethoxypolyethylene glycol
keywords: Antibacterial; CT contrast agent; Gold; Nanoparticles; Silver