title: Cell-SELEX aptamer for highly specific radionuclide molecular imaging of glioblastoma in vivo.
abstract: Glioblastoma (GBM) is the most frequent and aggressive primary adult brain tumor with poor prognosis. Epidermal growth factor receptor variant III (EGFRvIII) is the most common and highly oncogenic EGFR mutant in GBM. With the aim to generate specific molecular probes able to target EGFRvIII with high affinity, we selected four DNA aptamers (U2, U8, U19 and U31) specifically bound to U87-EGFRvIII cells that over expressed EGFRvIII with Kd values in the nanomole range by a cell-based systematic evolution of ligands by exponential enrichment (cell-SELEX) process. U87MG cells were introduced as control cells for counter selection. We further affirmed U2 and U8 identified EGFRvIII on the surface of target cells specifically. Then we radiolabeled U2 with 188Re to serve as a molecular imaging probe and observed 188Re -labeled U2 significantly targeted EGFRvIII over-expressing glioblastoma exnografts in mice. In conclusion, aptamers obtained from whole cell-SELEX strategy have great potential as molecular imaging probes that are probably beneficial to GBM diagnoses.
authors.affiliation: Department of Neurobiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.
authors.forename: Xidong
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Neurobiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.
authors.forename: Huiyu
authors.initials: H
authors.lastname: Liang
authors.affiliation: Department of Neurobiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Yuan
authors.affiliation: Department of Neurobiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.
authors.forename: Shuji
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Neurobiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Nuclear Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Guiping
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Yusheng
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Department of Neurobiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.
authors.forename: Xingmei
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001483:Base Sequence; D001932:Brain Neoplasms / Q000000981:diagnostic imaging* / Q000235:genetics; D045744:Cell Line, Tumor; D015342:DNA Probes / Q000235:genetics; D066246:ErbB Receptors / Q000235:genetics; D005909:Glioblastoma / Q000000981:diagnostic imaging* / Q000235:genetics; D006801:Humans; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals; D052156:SELEX Aptamer Technique
chemical_list: D015342:DNA Probes; D019275:Radiopharmaceuticals; C410723:epidermal growth factor receptor VIII; D066246:ErbB Receptors