title: Infection of differentiated airway epithelial cells from caprine lungs by viruses of the bovine respiratory disease complex.
abstract: Bovine respiratory syncytial virus (BRSV), bovine parainfluenza virus type 3 (BPIV3) and bovine herpesvirus type 1 (BHV-1) are important pathogens associated with the bovine respiratory disease complex (BRDC). Non-bovine ruminants such as goats may also be infected and serve as a virus reservoir to be considered in the development of control strategies. To evaluate the susceptibility of caprine airway epithelial cells to infection by viruses of BRDC, we established a culture system for differentiated caprine epithelial cells. For this purpose, we generated precision-cut lung slices (PCLS), in which cells are retained in their original structural configuration and remain viable for more than a week. The three bovine viruses were found to preferentially infect different cell types. Ciliated epithelial cells were the major target cells of BPIV3, whereas BHV-1 preferred basal cells. Cells infected by BRSV were detected in submucosal cell layers. This spectrum of susceptible cells is the same as that reported recently for infected bovine PCLS. While infection of caprine cells by BRSV and BPIV3 was as efficient as that reported for bovine cells, infection of caprine cells by BHV-1 required a tenfold higher dose of infectious virus as compared to infection of bovine airway cells. These results support the notion that non-bovine ruminants may serve as a reservoir for viruses of BRDC and introduce a culture system to analyze virus infection of differentiated airway epithelial cells from the caprine lung.
authors.affiliation: Institute of Virology, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: Jana
authors.initials: J
authors.lastname: Kirchhoff
authors.affiliation: Institute of Virology, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Uhlenbruck
authors.affiliation: Institute of Molecular Biology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany.
authors.forename: Günther M
authors.initials: GM
authors.lastname: Keil
authors.affiliation: Institute of Virology, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: Christel
authors.initials: C
authors.lastname: Schwegmann-Wessels
authors.affiliation: Clinic for Swine and Small Ruminants, Forensic Medicine and Ambulatory Service, University of Veterinary Medicine, Hannover, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Ganter
authors.affiliation: Institute of Virology, University of Veterinary Medicine Hannover, Hannover, Germany. Electronic address: georg.herrler@tiho-hannover.de.
authors.forename: Georg
authors.initials: G
authors.lastname: Herrler
mesh_terms: D000818:Animals; D048090:Bovine Respiratory Disease Complex / Q000821:virology*; D002417:Cattle; D002478:Cells, Cultured; D004197:Disease Reservoirs / Q000662:veterinary*; D004847:Epithelial Cells / Q000821:virology; D015511:Goat Diseases / Q000821:virology*; D006041:Goats; D007242:Herpesvirus 1, Bovine / Q000502:physiology; D054884:Host-Pathogen Interactions; D029101:Parainfluenza Virus 3, Bovine / Q000502:physiology; D020545:Respiratory Mucosa / Q000166:cytology / Q000821:virology*; D018112:Respiratory Syncytial Virus, Bovine / Q000502:physiology
keywords: Bovine herpesvirus type 1; Bovine parainfluenza virus type 3; Bovine respiratory disease complex; Bovine respiratory syncytial virus; Goat respiratory disease; Precision-cut lung slices