title: Two alternative ways of start site selection in human norovirus reinitiation of translation.
abstract: The calicivirus minor capsid protein VP2 is expressed via termination/reinitiation. This process depends on an upstream sequence element denoted termination upstream ribosomal binding site (TURBS). We have shown for feline calicivirus and rabbit hemorrhagic disease virus that the TURBS contains three sequence motifs essential for reinitiation. Motif 1 is conserved among caliciviruses and is complementary to a sequence in the 18 S rRNA leading to the model that hybridization between motif 1 and 18 S rRNA tethers the post-termination ribosome to the mRNA. Motif 2 and motif 2* are proposed to establish a secondary structure positioning the ribosome relative to the start site of the terminal ORF. Here, we analyzed human norovirus (huNV) sequences for the presence and importance of these motifs. The three motifs were identified by sequence analyses in the region upstream of the VP2 start site, and we showed that these motifs are essential for reinitiation of huNV VP2 translation. More detailed analyses revealed that the site of reinitiation is not fixed to a single codon and does not need to be an AUG, even though this codon is clearly preferred. Interestingly, we were able to show that reinitiation can occur at AUG codons downstream of the canonical start/stop site in huNV and feline calicivirus but not in rabbit hemorrhagic disease virus. Although reinitiation at the original start site is independent of the Kozak context, downstream initiation exhibits requirements for start site sequence context known for linear scanning. These analyses on start codon recognition give a more detailed insight into this fascinating mechanism of gene expression.
authors.affiliation: Institut für Immunologie, Friedrich-Loeffler-Institut, Südufer 10, 17493 Greifswald, Insel Riems, Germany. Electronic address: christine.luttermann@fli.bund.de.
authors.forename: Christine
authors.initials: C
authors.lastname: Luttermann
authors.affiliation: Institut für Immunologie, Friedrich-Loeffler-Institut, Südufer 10, 17493 Greifswald, Insel Riems, Germany. Electronic address: gregor.meyers@fli.bund.de.
authors.forename: Gregor
authors.initials: G
authors.lastname: Meyers
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003062:Codon; D006224:Cricetinae; D008969:Molecular Sequence Data; D029322:Norovirus / Q000502:physiology*; D016366:Open Reading Frames; D014176:Protein Biosynthesis / Q000502:physiology*; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D013728:Terminator Regions, Genetic; D014764:Viral Proteins / Q000235:genetics
chemical_list: D003062:Codon; D012337:RNA, Ribosomal, 18S; D014764:Viral Proteins
keywords: Calicivirus; Gene Expression; Norovirus; Protein Expression; RNA Structure; RNA Viruses; Reinitiation of Translation; TURBS; Translation; Viral Protein