title: Quantitative analysis of D-(+)-glucose in fruit juices using diffusion ordered-1H nuclear magnetic resonance spectroscopy.
abstract: This study works on D-(+)-glucose quantitative analysis using diffusion ordered-quantitative (1)H nuclear magnetic resonance spectroscopy (DOSY-qNMR), by which an analyte could be distinguished from interferences based upon a characteristic diffusion coefficient (D) in gradient magnetic fields. The D value of D-(+)-glucose in deuterium oxide at 30°C was 5.6 × 10(-10) m(2)/s at a field gradient pulse of between 5.0 × 10(-2) and 3.0 × 10(-1) T/m, distinguished from fructose, sucrose and starch. Good linearity (r(2) = 0.9998) was obtained between D-(+)-glucose (0.5-20.0 g/L) and the ratio of the resonance area of α-C1 proton (5.21 ppm) in D-(+)-glucose to that of the β-C1 proton (5.25 ppm) in D-glucuronic acid (50.0 g/L) as an internal standard. The DOSY-qNMR method was successfully applied to quantify D-(+)-glucose in orange juice (18.3 ± 1.0 g/L), apple juice (26.3 ± 0.4 g/L) and grape juice (45.6 ± 0.6 g/L); the values agreed well with a conventional F-kit glucose method.
authors.affiliation: Division of Bioresources and Biosciences, Faculty of Agriculture, Graduate School of Kyushu University.
authors.forename: Ruge
authors.initials: R
authors.lastname: Cao
authors.forename: Fusae
authors.initials: F
authors.lastname: Komura
authors.forename: Airi
authors.initials: A
authors.lastname: Nonaka
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kato
authors.forename: Junji
authors.initials: J
authors.lastname: Fukumashi
authors.forename: Toshiro
authors.initials: T
authors.lastname: Matsui
mesh_terms: D001628:Beverages / Q000032:analysis*; D004058:Diffusion; D005638:Fruit / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy / Q000592:standards; D011522:Protons; D012015:Reference Standards; D013237:Stereoisomerism
chemical_list: D011522:Protons; D005947:Glucose