title: Targeting SUR1/Abcc8-type neuroendocrine KATP channels in pancreatic islet cells.
abstract: ATP-sensitive K+ (KATP) channels play a regulatory role in hormone-secreting pancreatic islet α-, β- and δ-cells. Targeted channel deletion would assist analysis and dissection of the intraislet regulatory network. Toward this end Abcc8/Sur1 flox mice were generated and tested by crossing with glucagon-(GCG)-cre mice to target α-cell KATP channels selectively. Agonist resistance was used to quantify the percent of α-cells lacking channels. 41% of Sur1(loxP/loxP);GCG-cre+ and ∼64% of Sur1(loxP/-);GCG-cre+ α-cells lacked KATP channels, while ∼65% of α-cells expressed enhanced yellow fluorescent protein (EYFP) in ROSA-EYFP/GCG-cre matings. The results are consistent with a stochastic two-recombination event mechanism and a requirement that both floxed alleles are deleted.
authors.affiliation: Pacific Northwest Diabetes Research Institute, Seattle, Washington, United States of America.
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Nakamura
authors.affiliation: Pacific Northwest Diabetes Research Institute, Seattle, Washington, United States of America.
authors.forename: Joseph
authors.initials: J
authors.lastname: Bryan
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D005260:Female; D018390:Gene Targeting / Q000379:methods*; D019426:Integrases / Q000378:metabolism; D007515:Islets of Langerhans / Q000166:cytology* / Q000378:metabolism*; D054086:KATP Channels / Q000235:genetics*; D008297:Male; D051379:Mice; D057054:Molecular Imaging; D010641:Phenotype; D064233:Sulfonylurea Receptors / Q000235:genetics*
chemical_list: C577508:Abcc8 protein, mouse; D054086:KATP Channels; D064233:Sulfonylurea Receptors; C045073:Cre recombinase; D019426:Integrases; D002118:Calcium