title: Monosaccharide composition of glycans based on Q-HSQC NMR.
abstract: Glycans have essential functions related to structural architecture and specific cell surface phenomena, such as differentiation, biosignalling, recognition and cell-cell interaction, with the carbohydrate structure determining main function in the cell. Due to the importance of the primary structure, the monosaccharide composition is crucial to show the glycan structure. We now present a method for complex carbohydrates based on NMR spectroscopy, which has shown to give similar results to those obtained by the classic GC-MS-carboxy-reduction/deuterium labeling approach. Quantitative HSQC, through JCH dependence showed 155 Hz as the best value for (1)H/(13)C anomeric aldoses, allowing milli-microM detection using conventional inverse probe heads. Combining the quantification of native monosaccharide units of the glycan and those from the hydrolyzed product, a strong correlation occurs between the molecular mobility of the monosaccharide units, giving rise to some insights on the dynamic properties of the parent glycan.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba 81531-980, Brazil. Electronic address: sassaki@ufpr.br.
authors.forename: Guilherme Lanzi
authors.initials: GL
authors.lastname: Sassaki
authors.affiliation: Instituto di Richerche Chimiche e Biochimiche G. Ronzoni, Milan 20133, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Guerrini
authors.affiliation: Setor Litoral, Universidade Federal do Paraná, Matinhos 83260-000, Brazil.
authors.forename: Rodrigo Vassoler
authors.initials: RV
authors.lastname: Serrato
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba 81531-980, Brazil.
authors.forename: Arquimedes Paixão
authors.initials: AP
authors.lastname: Santana Filho
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba 81531-980, Brazil.
authors.forename: Juliane
authors.initials: J
authors.lastname: Carlotto
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba 81531-980, Brazil.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Simas-Tosin
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba 81531-980, Brazil.
authors.forename: Thales Ricardo
authors.initials: TR
authors.lastname: Cipriani
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba 81531-980, Brazil.
authors.forename: Marcello
authors.initials: M
authors.lastname: Iacomini
authors.affiliation: Instituto di Richerche Chimiche e Biochimiche G. Ronzoni, Milan 20133, Italy.
authors.forename: Giangiacomo
authors.initials: G
authors.lastname: Torri
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba 81531-980, Brazil.
authors.forename: Philip Albert James
authors.initials: PA
authors.lastname: Gorin
mesh_terms: D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009005:Monosaccharides / Q000032:analysis; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D009005:Monosaccharides; D011134:Polysaccharides
keywords: Carbohydrate quantification; Dynamics; Glycoconjugates; Mutarotation; Polysaccharides