title: Intrinsic healing response of the human anterior cruciate ligament: an histological study of reattached ACL remnants.
abstract: A reattachment of the tibial remnant of the torn anterior cruciate ligament (ACL) to the posterior cruciate ligament is sometimes observed during surgery and apparently implies that the human ACL does have a healing response. The aim of this study was to investigate whether this reattachment tissue has similar histological characteristics of a healing response as the medial collateral ligament (MCL), which can heal spontaneously. Standard histology and immunostaining of α-smooth muscle actin and collagen type 3 was performed. The results shows that the reattached tissue has typical characteristics of a healing response: there attached ACL remnant could not be released by forceful traction; microscopy showed that the collagen fibers of the reattached tissue were disorganized with no preferred direction; increased neovascularization; the presence of lipid vacuoles; the mean number of cells within the biopsy tissue was 631±269 cells per mm2; and 68±20% was expressing α-SMA; semi-quantitative analysis of collagen type 3 expression showed that collagen type 3 had an high expression with an average score of 3. In conclusion, this study shows that the human proximal 1/3 ACL has an intrinsic healing response with typical histological characteristics similar to the MCL.
authors.forename: Duy Tan
authors.initials: DT
authors.lastname: Nguyen
authors.forename: Tamara H
authors.initials: TH
authors.lastname: Ramwadhdoebe
authors.forename: Cor P
authors.initials: CP
authors.lastname: van der Hart
authors.forename: Leendert
authors.initials: L
authors.lastname: Blankevoort
authors.forename: Paul Peter
authors.initials: PP
authors.lastname: Tak
authors.forename: Cornelis Niek
authors.initials: CN
authors.lastname: van Dijk
mesh_terms: D000199:Actins / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D016118:Anterior Cruciate Ligament / Q000502:physiology* / Q000601:surgery / Q000648:ultrastructure; D000070598:Anterior Cruciate Ligament Injuries; D024061:Collagen Type III / Q000096:biosynthesis; D005260:Female; D006801:Humans; D008297:Male; D017888:Medial Collateral Ligament, Knee / Q000502:physiology; D014945:Wound Healing / Q000502:physiology*
chemical_list: D000199:Actins; D024061:Collagen Type III