title: The inductive effect of ginsenoside F2 on hair growth by altering the WNT signal pathway in telogen mouse skin.
abstract: This study was conducted to confirm the possibility of using minor ginseng saponin F2 by oral administration on hair anagen induction effects. The signaling pathway and anagen induction effect of ginsenoside F2 were investigated and compared with finasteride on the effect of hair growth induction. The cell-based MTT assay results indicated that the proliferation rates of HHDPC and HaCaT treated with F2 significantly increased by 30% compared with the finasteride-treated group. A western blot study showed that the expression of β-catenin Lef-1 and DKK-1 increased by 140, 200% and decreased by 40% in the F2-treated group, respectively compared to that of finasteride-treated group. C57BL/6 mice were subjected to the same treatments. The hair growth promotion rates were compared with groups treated with finasteride, which was 20% higher in the F2-treated group. Tissue histological analysis results showed the number of hair follicles, thickness of the epidermis, and follicles of the anagen phase which increased in the F2-treated group, compared with the finasteride-treated groups. Moreover, the effect of F2 on hair growth was confirmed through the immunofluorescence (IF) methods indicating the expression aspect of Wnt signal pathway-related factors in the tissue of C57BL/6 mouse. Our results considered the expression increase in β-catenin, Lef-1 which was suggested as a major factor related to the development and growth of hair follicle and the decrease in DKK-1 when entering catagen by F2. As the data showed, F2 might be a potential new therapeutic source for anagen induction and hair growth through the Wnt signal pathway.
authors.affiliation: Department of Oriental Medicinal Materials & Processing, College of Life Science, Kyung Hee University, 1 Seocheon-Dong, Yongin, 446-701, South Korea.
authors.forename: Heon-Sub
authors.initials: HS
authors.lastname: Shin
authors.affiliation: Department of Oriental Medicinal Materials & Processing, College of Life Science, Kyung Hee University, 1 Seocheon-Dong, Yongin, 446-701, South Korea.
authors.forename: Sang-Yong
authors.initials: SY
authors.lastname: Park
authors.affiliation: Department of Oriental Medicinal Materials & Processing, College of Life Science, Kyung Hee University, 1 Seocheon-Dong, Yongin, 446-701, South Korea.
authors.forename: Eun-Son
authors.initials: ES
authors.lastname: Hwang
authors.affiliation: Department of Oriental Medicinal Materials & Processing, College of Life Science, Kyung Hee University, 1 Seocheon-Dong, Yongin, 446-701, South Korea.
authors.forename: Don-Gil
authors.initials: DG
authors.lastname: Lee
authors.affiliation: Department of Oriental Medicinal Materials & Processing, College of Life Science, Kyung Hee University, 1 Seocheon-Dong, Yongin, 446-701, South Korea.
authors.forename: Hyun-Geun
authors.initials: HG
authors.lastname: Song
authors.affiliation: Department of Oriental Medicinal Materials & Processing, College of Life Science, Kyung Hee University, 1 Seocheon-Dong, Yongin, 446-701, South Korea.
authors.forename: Gafurjon T
authors.initials: GT
authors.lastname: Mavlonov
authors.affiliation: Department of Oriental Medicinal Materials & Processing, College of Life Science, Kyung Hee University, 1 Seocheon-Dong, Yongin, 446-701, South Korea. Electronic address: drhoo@khu.ac.kr.
authors.forename: Tae-Hoo
authors.initials: TH
authors.lastname: Yi
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D036145:Ginsenosides / Q000008:administration & dosage / Q000494:pharmacology*; D006197:Hair / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D051821:Lymphoid Enhancer-Binding Factor 1 / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012867:Skin / Q000166:cytology / Q000187:drug effects; D060449:Wnt Signaling Pathway / Q000187:drug effects*; D051176:beta Catenin / Q000378:metabolism
chemical_list: C501127:Dkk1 protein, mouse; D036145:Ginsenosides; D036341:Intercellular Signaling Peptides and Proteins; C497574:Lef1 protein, mouse; D051821:Lymphoid Enhancer-Binding Factor 1; D051176:beta Catenin; C407883:ginsenoside F2
keywords: DKK-1; Finasteride; Ginsenoside F2; Hair growth; β-catenin