title: Epigallocatechin-3-gallate inhibits tax-dependent activation of nuclear factor kappa B and of matrix metalloproteinase 9 in human T-cell lymphotropic virus-1 positive leukemia cells.
abstract: Epigallocatechin-3-gallate (EGCG) is the most abundant polyphenol molecule from green tea and is known to exhibit antioxidative as well as tumor suppressing activity. In order to examine EGCG tumor invasion and suppressing activity against adult T-cell leukemia (ATL), two HTLV-1 positive leukemia cells (HuT-102 and C91- PL) were treated with non-cytotoxic concentrations of EGCG for 2 and 4 days. Proliferation was significantly inhibited by 100 μM at 4 days, with low cell lysis or cytotoxicity. HTLV-1 oncoprotein (Tax) expression in HuT- 102 and C91-PL cells was inhibited by 25 μM and 125 μM respectively. The same concentrations of EGCG inhibited NF-kB nuclearization and stimulation of matrix metalloproteinase-9 (MMP-9) expression in both cell lines. These results indicate that EGCG can inhibit proliferation and reduce the invasive potential of HTLV-1- positive leukemia cells. It apparently exerted its effects by suppressing Tax expression, manifested by inhibiting the activation of NF-kB pathway and induction of MMP-9 transcription in HTLV-1 positive cells.
authors.affiliation: Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Kingdom of Saudi Arabia E-mail : sharakeh@gmail.com.
authors.forename: Steve
authors.initials: S
authors.lastname: Harakeh
authors.forename: Mona
authors.initials: M
authors.lastname: Diab-Assaf
authors.forename: Rania
authors.initials: R
authors.lastname: Azar
authors.forename: Hani Mutlak Abdulla
authors.initials: HM
authors.lastname: Hassan
authors.forename: Safwan
authors.initials: S
authors.lastname: Tayeb
authors.forename: Khalil
authors.initials: K
authors.lastname: Abou-El-Ardat
authors.forename: Ghazi Abdullah
authors.initials: GA
authors.lastname: Damanhouri
authors.forename: Ishtiaq
authors.initials: I
authors.lastname: Qadri
authors.forename: Adel
authors.initials: A
authors.lastname: Abuzenadah
authors.forename: Adeel
authors.initials: A
authors.lastname: Chaudhary
authors.forename: Taha
authors.initials: T
authors.lastname: Kumosani
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Niedzwiecki
authors.forename: Mathias
authors.initials: M
authors.lastname: Rath
authors.forename: Haitham
authors.initials: H
authors.lastname: Yacoub
authors.forename: Esam
authors.initials: E
authors.lastname: Azhar
authors.forename: Elie
authors.initials: E
authors.lastname: Barbour
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D016356:Gene Products, tax / Q000096:biosynthesis; D015368:Human T-lymphotropic virus 1 / Q000472:pathogenicity; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000188:drug therapy* / Q000821:virology; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis* / Q000235:genetics; D016328:NF-kappa B / Q000096:biosynthesis* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects; D015533:Transcriptional Activation; D014568:Urokinase-Type Plasminogen Activator / Q000037:antagonists & inhibitors
chemical_list: D000975:Antioxidants; D016356:Gene Products, tax; D016328:NF-kappa B; D018696:Neuroprotective Agents; D002392:Catechin; C045651:epigallocatechin gallate; D014568:Urokinase-Type Plasminogen Activator; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9