title: Spread of multidrug-resistant Enterococcus faecalis within the household setting.
abstract: Advances in veterinary medicine have resulted in the survival of many animals with severe illness or infectious diseases. In addition, increased usage of antimicrobial agents for veterinary purposes has contributed to the worldwide problem of increasing antimicrobial resistance. The objective of this study was to contribute to better understand the potential and implications for the spread of antimicrobial-resistant enterococci between pets receiving antimicrobial treatments and their owners. Three household aggregates (HA A, B, and C) were selected for this study. Information was collected on individual and clinical parameters of both humans and animals that cohabit. For this study, samples of feces, oral secretions, skin and fur of pets, as well as owners' feces and hands and exposed household surfaces and objects were also collected. All enterococci isolates were analyzed for antimicrobial susceptibility. Based on the antimicrobial resistance patterns and origin of isolates, ERIC-PCR analysis was performed on selected isolates to evaluate phylogenetic relationships. In all three HA, Enterococcus faecalis clonal spread was detected between pets and the respective owners, confirming the in-home interanimal species dissemination. Additionally, fecal enterococci colonization of other body parts of the same animal and dissemination of those same enterococci to household surfaces and objects were also observed. Our results demonstrate that enterococcal clones were found in pets in multiple body sites, their human cohabitants, and shared domestic objects.
authors.affiliation: 1 Veterinary Clinics Department-UPVET, Abel Salazar Institute for the Biomedical Sciences (ICBAS), University of Porto , Porto, Portugal .
authors.forename: Liliana
authors.initials: L
authors.lastname: Leite-Martins
authors.forename: Diana
authors.initials: D
authors.lastname: Meireles
authors.forename: Lucinda J
authors.initials: LJ
authors.lastname: Bessa
authors.forename: Ângelo
authors.initials: Â
authors.lastname: Mendes
authors.forename: Augusto J
authors.initials: AJ
authors.lastname: de Matos
authors.forename: Paulo Martins
authors.initials: PM
authors.lastname: da Costa
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004197:Disease Reservoirs / Q000382:microbiology*; D004285:Dogs; D013293:Enterococcus faecalis / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology; D005260:Female; D024841:Fluoroquinolones / Q000494:pharmacology; D016908:Gram-Positive Bacterial Infections / Q000453:epidemiology / Q000382:microbiology / Q000635:transmission* / Q000662:veterinary*; D006197:Hair / Q000382:microbiology; D006801:Humans; D008297:Male; D008795:Metronidazole / Q000494:pharmacology; D010802:Phylogeny; D011174:Portugal / Q000453:epidemiology; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D047090:beta-Lactams; D008795:Metronidazole