title: Comparative efficacy of Belzer or Euro-Collins solutions for pancreatic preservation during cold ischemic storage in rats.
abstract: OBJECTIVE
  To compare the efficacy of different types of solutions (Belzer or Euro-Collins) for the preservation of rat pancreas during cold ischemia.
  
  
  METHODS
  Thirty Wistar rats were divided into three groups according to the perfusion or storage solution: Group E (perfusion and storage in Euro-Collins solution); Group B (perfusion and storage in Belzer solution) and Group BE (Perfusion in Belzer solution and storage in Euro-Collins solution). After perfusion, the pancreas was excised and stored at 4˚C for 18 hours. Amylase was measured at 6, 12 and 18h, and histological analysis of the pancreas was performed after 18h of cold storage.
  
  
  RESULTS
  Amylase was elevated and comparable in Groups E and BE after 12 and 18 hours of ischemia (p<0.05). In the exocrine pancreas, histological differences in the amount of necrosis (p=0.049), lymphocytic infiltrate (p<0.001) and neutrophilic infiltrate (p=0.004) were observed, with more favorable features present in Group B. In the endocrine pancreas, Group B showed less edema (p<0.001), but other parameters were similar among all groups.
  
  
  CONCLUSIONS
  The Euro-Collins solution is inferior to the Belzer solution for the preservation of rat pancreas during cold ischemia.
authors.affiliation: Sao Paulo Federal University, Sao PauloSP, Brazil, Fellow Master degree, Postgraduate Program in Interdisciplinary Surgical Sciences, Sao Paulo Federal University(UNIFESP), Sao Paulo-SP, Brazil. Surgical procedures, acquisition and interpretation of data, manuscript writing.
authors.forename: Rogério Renato
authors.initials: RR
authors.lastname: Perez
authors.affiliation: UNIFESP, Paulista School of Medicine, Department of Surgery, Sao PauloSP, Brazil, PhD, Associate Professor, Gastroenterological Surgery Division, Department of Surgery, Paulista School of Medicine (EPM), UNIFESP, Sao Paulo-SP, Brazil. Interpretation of data.
authors.forename: Alberto
authors.initials: A
authors.lastname: Goldenberg
authors.affiliation: UNIFESP, EPM, Department of Surgery, Sao PauloSP, Brazil, PhD, Associate Professor, Pediatric Surgery Division, Department of Surgery, EPM-UNIFESP, Sao Paulo-SP, Brazil. Interpretation of data, critical revision.
authors.forename: Alcides Augusto Salzedas
authors.initials: AA
authors.lastname: Netto
authors.affiliation: UNIFESP, EPM, Department of Surgery, Sao PauloSP, Brazil, PhD, Associate Professor, Gastroenterological Surgery Division, Liver and Pancreas Transplant Unit, Department of Surgery, EPM-UNIFESP, Sao Paulo-SP, Brazil. Conception and design of the study, manuscript writing, critical revision.
authors.forename: Adriano Miziara
authors.initials: AM
authors.lastname: Gonzalez
mesh_terms: D000241:Adenosine; D000493:Allopurinol; D000681:Amylases / Q000032:analysis; D000818:Animals; D050377:Cold Ischemia / Q000379:methods*; D005260:Female; D005978:Glutathione; D006982:Hypertonic Solutions; D007328:Insulin; D023421:Models, Animal; D009336:Necrosis; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D010179:Pancreas / Q000473:pathology; D011887:Raffinose; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D000681:Amylases; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose