title: Toxicity and localization studies of a potential photodynamic therapy agent in Drosophila.
abstract: Photodynamic therapy utilizes light, a photosensitizer, and molecular oxygen as a treatment modality for a variety of cancers. We have recently combined ruthenium(II) polypyridyl groups with a zinc(II) centered porphyrin as a new photosensitizer for the treatment of melanoma. In-vitro studies have indicated that this photosensitizer is toxic to melanoma cells when irradiated with low energy light; however, it is nontoxic to normal cells under similar conditions. To determine the toxicity and cell viability of this compound in-vivo we present, herein, a study using Drosophila melanogaster. In the absence of light, the new photosensitizer shows no discernible effects to fly larvae at various concentrations of compound and stages of larval development. When the larvae were fed the photosensitizer it was observed, by fluorescence microscopy, that the compound passes through the cell membrane and localizes in the cytosol at lower concentrations and the nucleus at slightly higher concentrations indicating that the compound is not immediately metabolized.
authors.affiliation: Department of Chemistry, University of Dayton, Dayton, Ohio, 45469; Department of Biology, University of Dayton, Dayton, Ohio, 45469.
authors.forename: Joshua
authors.initials: J
authors.lastname: Yoho
authors.forename: Colette
authors.initials: C
authors.lastname: Stroh
authors.forename: Shawn
authors.initials: S
authors.lastname: Swavey
authors.forename: Madhuri
authors.initials: M
authors.lastname: Kango-Singh
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D004331:Drosophila melanogaster; D004354:Drug Screening Assays, Antitumor; D007814:Larva / Q000187:drug effects / Q000378:metabolism; D008665:Metalloporphyrins / Q000493:pharmacokinetics / Q000633:toxicity*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000493:pharmacokinetics / Q000633:toxicity*; D012428:Ruthenium / Q000493:pharmacokinetics / Q000633:toxicity*; D015032:Zinc / Q000493:pharmacokinetics / Q000633:toxicity*
chemical_list: D008665:Metalloporphyrins; D017319:Photosensitizing Agents; D012428:Ruthenium; D015032:Zinc
keywords: cell toxicity; imaginal discs; photosensitizer; porphyrin; zinc, ruthenium