title: Disturbance of antioxidant capacity produced by beauvericin in CHO-K1 cells.
abstract: Glutathione (GSH) levels, glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione-S-transferase (GST) as antioxidant defense system were evaluated in CHO-K1 cells after beauvericin (BEA) exposure. The effect of N-acetyl-cysteine (NAC) pre-treatment was assessed. GSH levels significantly decrease 18% and 29% after 5 μM of BEA in fresh medium and NAC pre-treatment, respectively compared to their controls. The GPx activity increased significantly from 35% to 66% in fresh medium and 20% in NAC pre-treatment. GR activity decreased after 5 μM of BEA up to 43% and 53% in fresh medium and NAC pre-treatment, respectively. The GST activity increased in fresh medium (from 61% to 89%) and decreased (from 22% to 35%) after NAC pre-treatment. Comparing BEA exposure in fresh medium and NAC pre-treatment, GSH levels, GPx activity and GST activity increased 716%, 458% and 206%, respectively respect to fresh medium; conversely no changes were observed in GR activity. In addition, NAC is an effective scavenger of BEA. GSH and related enzymes play an antioxidant role in the defense system of CHO-K1 cells exposed to BEA.
authors.affiliation: Laboratory of Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andres Estelles s/n, 46100 Burjassot, Valencia, Spain. Electronic address: beatriz.mallebrera@uv.es.
authors.forename: B
authors.initials: B
authors.lastname: Mallebrera
authors.affiliation: Laboratory of Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andres Estelles s/n, 46100 Burjassot, Valencia, Spain.
authors.forename: G
authors.initials: G
authors.lastname: Font
authors.affiliation: Laboratory of Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andres Estelles s/n, 46100 Burjassot, Valencia, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Ruiz
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D016466:CHO Cells; D049109:Cell Proliferation / Q000187:drug effects; D003412:Cricetulus; D047630:Depsipeptides / Q000633:toxicity*; D005978:Glutathione / Q000032:analysis; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D047630:Depsipeptides; C004456:beauvericin; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005978:Glutathione; D000111:Acetylcysteine
keywords: Beauvericin; CHO-K1 cells; Cytotoxicity; Enzymes; GSH; Oxidative stress