title: Matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis of Escherichia coli categories.
abstract: The mass profiles of cell-free extracts of 180 commensal and pathogenic strains of Escherichia coli were determined by MALDI-TOF mass spectrometry (MS). While some peaks were highly conserved in all E. coli, several peaks occurred only in some strains, showing heterogeneity among them. We did not detect strain-specific peaks for any of the E. coli categories tested. However, review of the fully conserved and the variable peaks suggested that MALDI-TOF MS has the potential to distinguish commensal and uropathogenic E. coli strains. Additionally, eight Shigella sonnei isolates were tested and found to be indistinguishable from E. coli by MALDI-TOF MS under the test conditions.
authors.affiliation: Departamento de Patologia Médica, Universidade Federal do Paraná, Curitiba, PR, Brasil.
authors.forename: C B
authors.initials: CB
authors.lastname: Dallagassa
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, PR, Brasil.
authors.forename: L F
authors.initials: LF
authors.lastname: Huergo
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, PR, Brasil.
authors.forename: M I
authors.initials: MI
authors.lastname: Stets
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, PR, Brasil.
authors.forename: F O
authors.initials: FO
authors.lastname: Pedrosa
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, PR, Brasil.
authors.forename: E M
authors.initials: EM
authors.lastname: Souza
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, PR, Brasil.
authors.forename: L M
authors.initials: LM
authors.lastname: Cruz
authors.affiliation: Departamento de Patologia Médica, Universidade Federal do Paraná, Curitiba, PR, Brasil.
authors.forename: F E A
authors.initials: FE
authors.lastname: Assis
authors.affiliation: Departamento de Patologia Médica, Universidade Federal do Paraná, Curitiba, PR, Brasil.
authors.forename: S
authors.initials: S
authors.lastname: Wolf
authors.affiliation: Departamento de Patologia Médica, Universidade Federal do Paraná, Curitiba, PR, Brasil.
authors.forename: W
authors.initials: W
authors.lastname: Volanski
authors.affiliation: Departamento de Patologia Médica, Universidade Federal do Paraná, Curitiba, PR, Brasil.
authors.forename: G
authors.initials: G
authors.lastname: Picheth
authors.affiliation: Instituto Federal de Educação, Ciência e Tecnologia de São Paulo, Matão, SP, Brasil.
authors.forename: C P
authors.initials: CP
authors.lastname: Pigatto-Denardi
authors.affiliation: Laboratório Central do Estado do Paraná, Curitiba, PR, Brasil.
authors.forename: S M S S
authors.initials: SM
authors.lastname: Farah
authors.affiliation: Departamento de Patologia Médica, Universidade Federal do Paraná, Curitiba, PR, Brasil fpicheth@ufpr.br.
authors.forename: C M T
authors.initials: CM
authors.lastname: Fadel-Picheth
mesh_terms: D002474:Cell-Free System; D004926:Escherichia coli / Q000737:chemistry* / Q000145:classification / Q000472:pathogenicity; D006801:Humans; D012764:Shigella sonnei / Q000737:chemistry / Q000145:classification* / Q000472:pathogenicity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization