title: Reinitiation after translation of two upstream open reading frames (ORF) governs expression of the ORF35-37 Kaposi's sarcoma-associated herpesvirus polycistronic mRNA.
abstract: The Kaposi's sarcoma-associated herpesvirus (KSHV) ORF36 protein kinase is translated as a downstream gene from the ORF35-37 polycistronic mRNA via a unique mechanism involving short upstream open reading frames (uORFs) located in the 5' untranslated region. Here, we confirm that ORF35-37 is functionally dicistronic during infection and demonstrate that mutation of the dominant uORF restricts KSHV replication. Leaky scanning past the uORFs facilitates ORF35 expression, while a reinitiation mechanism after translation of the uORFs enables ORF36 expression.
authors.affiliation: Department of Plant and Microbial Biology, University of California, Berkeley, California, USA.
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Kronstad
authors.forename: Kevin F
authors.initials: KF
authors.lastname: Brulois
authors.forename: Jae U
authors.initials: JU
authors.lastname: Jung
authors.forename: Britt A
authors.initials: BA
authors.lastname: Glaunsinger
mesh_terms: D015967:Gene Expression Regulation, Viral / Q000235:genetics*; D019288:Herpesvirus 8, Human / Q000235:genetics* / Q000378:metabolism; D008957:Models, Genetic; D016366:Open Reading Frames / Q000235:genetics*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D011494:Protein Kinases; C492430:ORF36 protein kinase, Kaposi's sarcoma herpesvirus