title: Ultrasonication assisted lipid extraction from oleaginous microorganisms.
abstract: Various solvents, including water, hexane, methanol, and chloroform/methanol (1:1 v/v), were tested to identify the efficiency of lipid extraction from Trichosporon oleaginosus and an oleaginous fungal strain SKF-5 under ultrasonication (520 kHz 40 W and 50 Hz 2800 W) and compared with the conventional chloroform methanol (2:1 v/v) extraction method. The highest lipid recovery 10.2% and 9.3% with water, 43.2% and 33.2% with hexane, 75.7% and 65.1% with methanol, 100% and 100% w/w biomass with chloroform/methanol were obtained from T. oleaginosus and SKF-5 strain, respectively, at ultrasonication frequency 50 Hz and power input 2800 W. Ultrasonication chloroform/methanol extraction recovered total lipid in a short time (15 min) and low temperature (25°C). Whereas the conventional chloroform methanol extraction to achieve total lipid recovery required 12h at 60°C. Ultrasonication chloroform/methanol extraction would be a promising method of lipid extraction from the microorganisms.
authors.affiliation: INRS Eau, Terre et Environnement, 490, rue de la Couronne, Québec G1K 9A9, Canada.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Zhang
authors.affiliation: INRS Eau, Terre et Environnement, 490, rue de la Couronne, Québec G1K 9A9, Canada.
authors.forename: Song
authors.initials: S
authors.lastname: Yan
authors.affiliation: INRS Eau, Terre et Environnement, 490, rue de la Couronne, Québec G1K 9A9, Canada. Electronic address: rd.tyagi@ete.inrs.ca.
authors.forename: Rajeshwar D
authors.initials: RD
authors.lastname: Tyagi
authors.affiliation: INRS Eau, Terre et Environnement, 490, rue de la Couronne, Québec G1K 9A9, Canada.
authors.forename: Patrick
authors.initials: P
authors.lastname: Drogui
authors.affiliation: Department of Civil Engineering, University of Nebraska-Lincoln, N104 SEC, PO Box 886105, Lincoln, NE 68588-6105, United States.
authors.forename: Rao Y
authors.initials: RY
authors.lastname: Surampalli
mesh_terms: D008055:Lipids / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry; D014250:Trichosporon / Q000737:chemistry*; D014465:Ultrasonics
chemical_list: D008055:Lipids; D012997:Solvents
keywords: Biodiesel; Lipid extraction; Oleaginous microorganism; Ultrasonication