title: Caffeic acid phenethyl ester, a major component of propolis, suppresses high fat diet-induced obesity through inhibiting adipogenesis at the mitotic clonal expansion stage.
abstract: In the present study, we aimed to investigate the antiobesity effect of CAPE in vivo, and the mechanism by which CAPE regulates body weight in vitro. To confirm the antiobesity effect of CAPE in vivo, mice were fed with a high fat diet (HFD) with different concentrations of CAPE for 5 weeks. CAPE significantly reduced body weight gain and epididymal fat mass in obese mice fed a HFD. In accordance with in vivo results, Oil red O staining results showed that CAPE significantly suppressed MDI-induced adipogenesis of 3T3-L1 preadipocytes. FACS analysis results showed that CAPE delayed MDI-stimulated cell cycle progression, thereby contributing to inhibit mitotic clonal expansion (MCE), which is a prerequisite step for adipogenesis. Also, CAPE regulated the expression of cyclin D1 and the phosphorylation of ERK and Akt, which are upstream of cyclin D1. These results suggest that CAPE exerts an antiobesity effect in vivo, presumably through inhibiting adipogenesis at an early stage of adipogenesis.
authors.affiliation: Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, ‡WCU Biomodulation Major, Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University , Seoul 151-921, Republic of Korea.
authors.forename: Seung Ho
authors.initials: SH
authors.lastname: Shin
authors.forename: Sang Gwon
authors.initials: SG
authors.lastname: Seo
authors.forename: Soyun
authors.initials: S
authors.lastname: Min
authors.forename: Hee
authors.initials: H
authors.lastname: Yang
authors.forename: Eunjung
authors.initials: E
authors.lastname: Lee
authors.forename: Joe Eun
authors.initials: JE
authors.lastname: Son
authors.forename: Jung Yeon
authors.initials: JY
authors.lastname: Kwon
authors.forename: Shuhua
authors.initials: S
authors.lastname: Yue
authors.forename: Min-Yu
authors.initials: MY
authors.lastname: Chung
authors.forename: Kee-Hong
authors.initials: KH
authors.lastname: Kim
authors.forename: Ji-Xin
authors.initials: JX
authors.lastname: Cheng
authors.forename: Hyong Joo
authors.initials: HJ
authors.lastname: Lee
authors.forename: Ki Won
authors.initials: KW
authors.lastname: Lee
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D019440:Anti-Obesity Agents / Q000008:administration & dosage*; D002109:Caffeic Acids / Q000008:administration & dosage*; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects; D015536:Down-Regulation / Q000187:drug effects; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008938:Mitosis / Q000187:drug effects*; D009765:Obesity / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D010626:Phenylethyl Alcohol / Q000008:administration & dosage / Q000031:analogs & derivatives*; D011429:Propolis / Q000737:chemistry*
chemical_list: D019440:Anti-Obesity Agents; D002109:Caffeic Acids; D019938:Cyclin D1; D011429:Propolis; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol