title: MicroRNA-100 regulates SW620 colorectal cancer cell proliferation and invasion by targeting RAP1B.
abstract: MicroRNAs (miRNAs) have been demonstrated to play important roles in tumorigenesis of human cancer. Fewer studies have explored the roles of miR-100 on human colorectal cancer cell proliferation and invasion. In this study, we utilized real-time PCR to verify whether miR-100 was downregulated in human colorectal cancer tissues compared with matched adjacent normal tissues. Functional studies demonstrated that ectopic expression of miR-100 inhabits cell growth and invasion and induce apoptosis, whereas knockdown of miR-100 yielded the reverse phenotype. Mechanistic studies reveal that miR-100 repressed the activity of a reporter gene fused to the 3'-untranslated region (3'-UTR) of RAP1B, whereas miR-100 silencing upregulated the expression of the reporter gene. Furthermore, we also detected that RAP1B mRNA was inversely expressed with miR-100 in colorectal cancer tissues. These data indicate that the miR-100 plays a tumor suppressor role by regulating colorectal cancer cell growth and invasion phenotype, and could serve as a potential maker for colorectal cancer therapy.
authors.affiliation: Department of Colorectal and Anal Surgery, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Peng
authors.affiliation: Department of Colorectal and Anal Surgery, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Luo
authors.affiliation: Department of Pediatrics, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655, P.R. China.
authors.forename: Hu
authors.initials: H
authors.lastname: Hao
authors.affiliation: Guangdong Gastrointestinal Institute, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Colorectal and Anal Surgery, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655, P.R. China.
authors.forename: Shang-Kui
authors.initials: SK
authors.lastname: Xie
authors.affiliation: Department of Colorectal and Anal Surgery, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655, P.R. China.
authors.forename: Donglin
authors.initials: D
authors.lastname: Ren
authors.affiliation: Department of Gastrointestinal Anal Surgery and Institute of Gastroenterology, The Third Affiliated Hospital of Nanchang University, Nanchang 330008, P.R. China.
authors.forename: Benqiang
authors.initials: B
authors.lastname: Rao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics; D009362:Neoplasm Metastasis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D020668:rap GTP-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; C544266:MIRN100 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; C116753:RAP1B protein, human; D020668:rap GTP-Binding Proteins