title: Enhancement of cell viability by fabrication of macroscopic 3D hydrogel scaffolds using an innovative cell-dispensing technique supplemented by preosteoblast-laden micro-beads.
abstract: We propose a new cell-encapsulated dispensing method consisting of hydrogel struts, embedded with cell-laden micro-beads. To develop the scaffolds, we accommodated a three-axis robot dispensing system and aerosol spraying of a cross-linking agent to effect tentative surface gelation of hydrogel alginate struts. To show the feasibility of the method, we used pre-osteoblast (MC3T3-E1) cells. Using this technique, we obtained a reasonable cell viability (>90% after several culture periods) relative to that of a scaffold onto which cells were dispensed in the conventional manner, and successfully fabricated a realistic macroscopic pore-size in a controlled manner with 100% pore-interconnected 3D alginate hydrogel scaffolds of 20 mm × 20 mm × 6 mm.
authors.affiliation: Department of Bio-Mechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, South Korea.
authors.forename: Hyeongjin
authors.initials: H
authors.lastname: Lee
authors.affiliation: Department of Bio-Mechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, South Korea.
authors.forename: Seunghyun
authors.initials: S
authors.lastname: Ahn
authors.affiliation: Department of Surgery, College of Medicine, Hallym University, Hangang Sacred Heart Hospital, Seoul, South Korea.
authors.forename: Wook
authors.initials: W
authors.lastname: Chun
authors.affiliation: Department of Bio-Mechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, South Korea. Electronic address: gkimbme@skku.edu.
authors.forename: Geunhyung
authors.initials: G
authors.lastname: Kim
mesh_terms: D000336:Aerosols / Q000737:chemistry / Q000494:pharmacology; D000464:Alginates / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D057205:Automation, Laboratory; D002460:Cell Line; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D002470:Cell Survival; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000494:pharmacology; D020100:Hydrogels / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D008863:Microspheres; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D012371:Robotics; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000336:Aerosols; D000464:Alginates; D003432:Cross-Linking Reagents; D020100:Hydrogels
keywords: 3D scaffold; Alginate; Bone; Cell-laden beads; Tissue regeneration