title: Upconversion luminescence resonance energy transfer (LRET)-based biosensor for rapid and ultrasensitive detection of avian influenza virus H7 subtype.
abstract: Avian influenza viruses (AIV) with good adaptation and various mutations have threatened both human and animals' health. The H7 subtypes have the potential to cause pandemic threats to human health due to the highly pathogenic characteristics. Therefore, it is quite urgent to develop a novel biosensor for rapid and sensitive detection of H7 subtypes. In this work, a biosensor based on luminescence resonance energy transfer (LRET) from BaGdF5:Yb/Er upconversion nanoparticles (UCNPs) to gold nanoparticles (AuNPs) has been developed for rapid and sensitive H7 subtypes detection. The amino modified capture oligonucleotide probes are covalently linked to poly(ethylenimine) (PEI) modified BaGdF5:Yb/Er UCNPs. The thiol modified oligonucleotides with H7 hemagglutinin gene sequence are conjugated to surfaces of AuNPs. The hybridization process between complementary strands of H7 Hemagglutinin gene and its probe brings the energy donor and acceptor into close proximity, leading to the quenching of fluorescence of UCNPs. A linear response is obtained ranging from 10 pm to 10 nm and the limit of detection (LOD) is around 7 pm with detection time around 2 hours. This biosensor is expected to be a valuable diagnostic tool for rapid and sensitive detection of AIV.
authors.affiliation: Interdisciplinary Division of Biomedical Engineering, the Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, P. R. China.
authors.forename: Wei Wei
authors.initials: WW
authors.lastname: Ye
authors.forename: Ming-Kiu
authors.initials: MK
authors.lastname: Tsang
authors.forename: Xuan
authors.initials: X
authors.lastname: Liu
authors.forename: Mo
authors.initials: M
authors.lastname: Yang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Hao
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001717:Birds; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation / Q000379:methods*; D006046:Gold; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics*; D006801:Humans; D057916:Influenza A Virus, H7N1 Subtype / Q000235:genetics / Q000302:isolation & purification; D057917:Influenza A Virus, H7N2 Subtype / Q000235:genetics / Q000302:isolation & purification; D057920:Influenza A Virus, H7N3 Subtype / Q000235:genetics / Q000302:isolation & purification; D053126:Influenza A Virus, H7N7 Subtype / Q000235:genetics / Q000302:isolation & purification; D064766:Influenza A Virus, H7N9 Subtype / Q000235:genetics / Q000302:isolation & purification; D009980:Influenza A virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005585:Influenza in Birds / Q000175:diagnosis* / Q000821:virology; D007251:Influenza, Human / Q000175:diagnosis / Q000235:genetics; D057230:Limit of Detection; D049449:Luminescence; D053768:Metal Nanoparticles / Q000737:chemistry; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; C109963:hemagglutinin, avian influenza A virus; D006046:Gold
keywords: avian influenza virus detection; luminescence resonance energy transfer; nanoparticles; upconversion