title: Cell behavior on surface modified polydimethylsiloxane (PDMS).
abstract: Designing complex tissue culture systems requires cell alignment and directed extracellular matrix (ECM) and gene expression. Here, a micro-rough, polydimethylsiloxane (PDMS) surface, that also integrates a micro-pattern of 50 µm wide lines of fibronectin (FN) separated by 60 µm wide lines of bovine serum albumin (BSA), is developed. Human fibroblasts cultured on the rough, patterned substrate have aligned growth and a significant change in morphology when compared to cells on a flat, patterned surface. The rough PDMS topography significantly decreases cell area and induces the upregulation of several ECM related genes by two-fold when compared to cells cultured on flat PDMS. This study describes a simple surface engineering procedure for creating surface architecture for scaffolds to design and control the cell-surface interface.
authors.affiliation: Worcester Polytechnic Institute, Department of Chemistry and Biochemistry, 100 Institute Road, Worcester, MA 01609, USA.
authors.forename: Morgan M
authors.initials: MM
authors.lastname: Stanton
authors.forename: Johanna M
authors.initials: JM
authors.lastname: Rankenberg
authors.forename: Byung-Wook
authors.initials: BW
authors.lastname: Park
authors.forename: W Grant
authors.initials: WG
authors.lastname: McGimpsey
authors.forename: Christopher
authors.initials: C
authors.lastname: Malcuit
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Lambert
mesh_terms: D000818:Animals; D002417:Cattle; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D004129:Dimethylpolysiloxanes / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005353:Fibronectins / Q000494:pharmacology; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D018625:Microscopy, Atomic Force; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013499:Surface Properties
chemical_list: D015815:Cell Adhesion Molecules; D004129:Dimethylpolysiloxanes; D005353:Fibronectins; D012710:Serum Albumin, Bovine; C013830:baysilon
keywords: biomaterials; microstructure; morphology; proteins; surfaces