title: Autophagosome biogenesis in plants: roles of SH3P2.
abstract: The autophagy core machinery is essentially conserved in eukaryotic cells for autophagy regulation. However, the underlying mechanisms for autophagosome formation in plant cells remain elusive. We have recently demonstrated that SH3 domain-containing protein 2 (SH3P2), a BAR (Bin-Amphiphysin-Rvs) domain protein, functions as a novel regulator for autophagosome biogenesis in Arabidopsis thaliana. Using SH3P2 and its GFP fusion as probes, we have characterized the dynamics and structures of autophagosome formation in plant cells. The phagophore assembly site, marked by SH3P2, is identified as having a close connection with the ER. SH3P2 also binds to phosphatidylinositol 3-phosphate (PtdIns3P) and functions downstream of the phosphatidylinositol 3-kinase (PtdIns3K) complex. Thus, SH3P2 serves as a novel membrane-associated protein in regulating autophagosome formation in Arabidopsis thaliana.
authors.affiliation: School of Life Sciences; Center for Cell and Developmental Biology and State Key Laboratory of Agrobiotechnology; Chinese University of Hong Kong; Hong Kong, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhuang
authors.affiliation: School of Life Sciences; Center for Cell and Developmental Biology and State Key Laboratory of Agrobiotechnology; Chinese University of Hong Kong; Hong Kong, China; Shenzhen Research Institute; Chinese University of Hong Kong; Shenzhen, China.
authors.forename: Liwen
authors.initials: L
authors.lastname: Jiang
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000201:enzymology; D001343:Autophagy / Q000502:physiology*; D002450:Cell Communication / Q000502:physiology; D008565:Membrane Proteins / Q000378:metabolism*; D010588:Phagosomes / Q000378:metabolism*; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D018129:Phosphatidylinositol Phosphates; C055525:phosphatidylinositol 3-phosphate
keywords: Bin-Amphiphysin-Rvs domain; SH3 domain-containing protein; phagophore assembly site; phosphatidylinositol 3-kinase; phosphatidylinositol 3-phosphate; plant autophagosome formation