title: Inhibiting mast cell degranulation by HO-1 affects dendritic cell maturation in vitro.
abstract: OBJECTIVE
  Mast cell (MC) degranulation can break peripheral immune tolerance. However, its mechanism remains unclear. Our goal was to study the stabilization of MC membranes by heme oxygenase-1 (HO-1) in order to influence dendritic cell (DC) function.
  
  
  METHODS
  Mast cells and dendritic cells were prepared from 8-week-old to 10-week-old C57BL/6 mice; spleen mononuclear cells (SMCs) were prepared from 8-week-old to 10-week-old C57BL/6 and Balb/c mice.
  
  
  METHODS
  Mast cells were pretreated with PBS, DMSO, Hemin (50 μl/ml), and Znpp (50 μl/ml) for 8 h.
  
  
  METHODS
  Real-time PCR and western-blot tested the HO-1 of MC mRNA and protein. The co-stimulatory molecules of DCs (CD80, CD86, CD40) were measured by flow cytometry, and levels of TNF-α, IL-6, and IFN-γ were measured by ELISA. We set up a one-way mixed lymphocyte reaction (MLR) model to test the proliferation of SMCs after MC/DC interaction. *P < 0.05 (t test) was taken as the level of statistical significance.
  
  
  RESULTS
  MCs pretreated with hemin induced HO-1 mRNA and protein expression, then interacted with DCs; expression of the co-stimulatory molecules was attenuated. The TNF-α, IL-6, and IFN-γ levels in the co-culture system were decreased. These DCs couldn't stimulate the proliferation of SMCs.
  
  
  CONCLUSIONS
  Inhibiting MC degranulation by HO-1 restrained DC maturation and attenuated the proliferation of SMCs.
authors.affiliation: Department of General Surgery, Shanghai Tenth People's Hospital of Tong Ji University, Shanghai, China.
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Ma
authors.forename: Mu-qing
authors.initials: MQ
authors.lastname: Yang
authors.forename: Chun-feng
authors.initials: CF
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Ding
authors.forename: Ji-yu
authors.initials: JY
authors.lastname: Li
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D015550:Cell Degranulation / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D018920:Coculture Techniques; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000187:drug effects / Q000502:physiology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D051547:Heme Oxygenase-1 / Q000502:physiology*; D006427:Hemin / Q000494:pharmacology; D007108:Immune Tolerance; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000502:physiology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000502:physiology*; D008565:Membrane Proteins / Q000502:physiology*; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011524:Protoporphyrins / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D013154:Spleen / Q000166:cytology
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D011524:Protoporphyrins; D012333:RNA, Messenger; C017803:zinc protoporphyrin; D006427:Hemin; D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse; D004121:Dimethyl Sulfoxide