title: Oxidative stress and fatty acid profile in Wistar rats subjected to acute food restriction and refeeding with high-fat diets.
abstract: OBJECTIVE
  To assess oxidative stress and the profile of fatty acids incorporated into the hepatic tissue of animals refed with high-fat (HF) diets after acute food restriction.
  
  
  METHODS
  Fifty male Wistar rats were divided into five groups and fasting for 48 hours. One group was sacrificed without refeeding (NR), a control group (C) was refed with the standard AIN-93 diet and the remaining groups with HF diets respectively consisting of hydrogenated vegetable oil (PHVO), trans-free (TF) margarine and trans-free margarine enriched with ω-3 and ω-6 (O). After this period the animals were sacrificed for malondialdehyde (MDA), catalase and hepatic fatty acid determination.
  
  
  RESULTS
  The groups refed with HF diets showed elevation of MDA levels compared to the C group (p<0.001 for GVH and p<0.01 for TF and O). Hepatic catalase activity was higher in the TF and O groups compared to group C (p<0.05 for both). The amount of saturated fatty acids was lower in the PHVO and O groups compared to the remaining ones (p<0.001).
  
  
  CONCLUSIONS
  The consumption of high-fat diets after prolonged fasting favors oxidative imbalance in hepatic tissue.
authors.affiliation: University of Sao Paulo, Faculty of Medicine of Ribeirao Preto, Department of Internal Medicine, Ribeirao PretoSP, Brazil, Nutritionist, Department of Internal Medicine, Faculty of Medicine of Ribeirao Preto-University of Sao Paulo (FMRP-USP), Ribeirao Preto-SP, Brazil. Technical procedures, acquisition and interpretation of data, manuscript writing.
authors.forename: Ana Lígia da Silva
authors.initials: AL
authors.lastname: Nassar
authors.affiliation: University of Sao Paulo, Faculty of Medicine of Ribeirao Preto, Department of Internal Medicine, Ribeirao PretoSP, Brazil, Nutritionist, Department of Internal Medicine, Faculty of Medicine of Ribeirao Preto-University of Sao Paulo (FMRP-USP), Ribeirao Preto-SP, Brazil. Technical procedures, acquisition and interpretation of data, manuscript writing.
authors.forename: Luisa Pereira
authors.initials: LP
authors.lastname: Marot
authors.affiliation: USP, FMRP, Department of Internal Medicine, Ribeirao PretoSP, Brazil, Master, Laboratory of Nutrition and Metabolism, Department of Internal Medicine, FMRP-USP, Ribeirao Preto-SP, Brazil. Technical procedures, acquisition and interpretation of data.
authors.forename: Paula Payão
authors.initials: PP
authors.lastname: Ovidio
authors.affiliation: USP, FMRP, Department of Internal Medicine, Ribeirao PretoSP, Brazil, Fellow PhD degree, Postgraduate Program in Internal Medicine, Biomedical Investigation, Department of Internal Medicine, FMRP-USP, Ribeirao Preto-SP, Brazil. Technical procedures, acquisition and interpretation of data, statistical analysis, manuscript writing.
authors.forename: Gabriela Salim Ferreira de
authors.initials: GS
authors.lastname: Castro
authors.affiliation: USP, FMRP, Department of Internal Medicine, Ribeirao PretoSP, Brazil, PhD, Department of Internal Medicine, FMRP-USP, Ribeirao Preto-SP, Brazil. Design the protocol, manuscript writing, supervised all phases of the study, critical revision.
authors.forename: Alceu Afonso
authors.initials: AA
authors.lastname: Jordão
mesh_terms: D000818:Animals; D002374:Catalase / Q000502:physiology; D059305:Diet, High-Fat; D005215:Fasting / Q000502:physiology*; D005227:Fatty Acids / Q000032:analysis*; D005234:Fatty Liver / Q000378:metabolism; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D023421:Models, Animal; D018384:Oxidative Stress / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D013997:Time Factors
chemical_list: D005227:Fatty Acids; D008315:Malondialdehyde; D002374:Catalase