title: Biotin-streptavidin binding interactions of dielectric filled silicon bulk acoustic resonators for smart label-free biochemical sensor applications.
abstract: Sensor performance of a dielectric filled silicon bulk acoustic resonator type label-free biosensor is verified with biotin-streptavidin binding interactions as a model system. The mass sensor is a micromachined silicon square plate with a dielectric filled capacitive excitation mechanism. The resonance frequency of the biotin modified resonator decreased 315 ppm when exposed to streptavidin solution for 15 min with a concentration of 10(-7) M, corresponding to an added mass of 3.43 ng on the resonator surface. An additional control is added by exposing a bovine serum albumin (BSA)-covered device to streptavidin in the absence of the attached biotin. No resonance frequency shift was observed in the control experiment, which confirms the specificity of the detection. The sensor-to-sensor variability is also measured to be 4.3%. Consequently, the developed sensor can be used to observe in biotin-streptavidin interaction without the use of labelling or molecular tags. In addition, biosensor can be used in a variety of different immunoassay tests.
authors.affiliation: Institute of Microelectronics, Agency for Science, Technology and Research (A*STAR), Singapore 117685, Singapore. aheidari@ucdavis.edu.
authors.forename: Amir
authors.initials: A
authors.lastname: Heidari
authors.affiliation: Department of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore 639798, Singapore. yongjiny@ntu.edu.sg.
authors.forename: Yong-Jin
authors.initials: YJ
authors.lastname: Yoon
authors.affiliation: Department of Mechanical and Automotive Engineering, Seoul National University of Science and Technology, Seoul 139743, Korea. wtpark@seoultech.ac.kr.
authors.forename: Woo-Tae
authors.initials: WT
authors.lastname: Park
authors.affiliation: Department of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore 639798, Singapore. peichensu@ntu.edu.sg.
authors.forename: Pei-Chen
authors.initials: PC
authors.lastname: Su
authors.affiliation: Department of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore 639798, Singapore. jmiao@pmail.ntu.edu.sg.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Miao
authors.affiliation: Institute of Microelectronics, Agency for Science, Technology and Research (A*STAR), Singapore 117685, Singapore. tsaiml@ime.a-star.edu.sg.
authors.forename: Julius Tsai Ming
authors.initials: JT
authors.lastname: Lin
authors.affiliation: Institute of Microelectronics, Agency for Science, Technology and Research (A*STAR), Singapore 117685, Singapore. parkmk@ime.a-star.edu.sg.
authors.forename: Mi Kyoung
authors.initials: MK
authors.lastname: Park
mesh_terms: D000162:Acoustics / Q000295:instrumentation*; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D001710:Biotin / Q000378:metabolism*; D002417:Cattle; D004566:Electrodes; D004573:Electrolytes / Q000737:chemistry; D055767:Immobilized Proteins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008970:Molecular Weight; D011485:Protein Binding; D012825:Silicon / Q000737:chemistry*; D012996:Solutions; D013194:Staining and Labeling; D019809:Streptavidin / Q000378:metabolism*
chemical_list: D004573:Electrolytes; D055767:Immobilized Proteins; D012996:Solutions; D001710:Biotin; D019809:Streptavidin; D012825:Silicon