title: The effect of taurine on the relationship between NO, ADMA and homocysteine in endotoxin-mediated inflammation in HUVEC cultures.
abstract: The aim of our study was to investigate the effect of taurine on the relationship between nitric oxide (NO), asymmetric dimethylarginine (ADMA) and homocysteine (Hcy) in endotoxin-induced human umblical vein endothelial cell (HUVEC) cultures. For this reason, four groups were formed (n=12). Control group consists of HUVEC cultures without any treatment. Lipopolysaccharide (LPS) and LPS+taurine groups were treated with 10 μg/mL endotoxin, 5 μg/mL taurine and endotoxin+taurine (same doses), respectively. Nitrite/nitrate (NOx), ADMA and Hcy levels were measured. There was a significant increase of NOx, ADMA and Hcy in endotoxemia (p<0.05). Taurine treatment elevated NOx levels significantly (p<0.01) in taurine and LPS + taurine group compared to control group, while it reduced NOx levels compared to LPS group. In contrast, taurine decreased ADMA levels to the control level both in taurine and taurine+LPS group compared to LPS. Hcy levels increased significantly compared to taurine group (p<0.05) and did not change compared to LPS group. Taurine was effective on ADMA-NO relationship whereas no beneficial effect was observed in Hcy levels (p<0.05).
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine, Gazi University, Ankara, Turkey.
authors.forename: Ozge Tugce
authors.initials: OT
authors.lastname: Pasaoglu
authors.forename: Nurten
authors.initials: N
authors.lastname: Turkozkan
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ark
authors.forename: Belgin
authors.initials: B
authors.lastname: Polat
authors.forename: Mehmet
authors.initials: M
authors.lastname: Agilli
authors.forename: Halil
authors.initials: H
authors.lastname: Yaman
mesh_terms: D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism; D004731:Endotoxins / Q000633:toxicity*; D006710:Homocysteine / Q000096:biosynthesis*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D009569:Nitric Oxide / Q000096:biosynthesis*; D013654:Taurine / Q000494:pharmacology*
chemical_list: D004731:Endotoxins; D018836:Inflammation Mediators; D006710:Homocysteine; D013654:Taurine; D009569:Nitric Oxide