title: Characterization of Salmonella Enteritidis strains isolated from poultry and farm environments in Brazil.
abstract: Salmonella Enteritidis is a major causative agent of foodborne outbreaks worldwide. Using enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) and pulsed-field gel electrophoresis (PFGE), this study assessed the genetic relatedness, the pathogenic potential, and antimicrobial resistance in 60 strains isolated from chickens and the farm environment in Brazil between 2004 and 2010. The resulting concatenated dendrogram of the two methodologies distinguished the strains into two clusters. Some strains isolated from the two sources were indistinguishable. All the strains contained the 13 virulence markers investigated. Forty-four strains were resistant to nalidixic acid. Quinolone resistance presented by many strains suggests that quinolones may have been used to treat chickens. The high prevalence of virulence markers highlights the importance of poultry as vehicles of S. Enteritidis strains that have the potential to cause disease.
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto - Universidade de São Paulo, Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Ribeirão Preto, SP,Brazil.
authors.forename: F
authors.initials: F
authors.lastname: Campioni
authors.affiliation: AVIPA - Avicultura Integral e Patologia S/A, Chácara da Barra, Campinas, SP,Brazil.
authors.forename: M M
authors.initials: MM
authors.lastname: Zoldan
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto - Universidade de São Paulo, Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Ribeirão Preto, SP,Brazil.
authors.forename: J P
authors.initials: JP
authors.lastname: Falcão
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D001938:Brazil / Q000453:epidemiology; D002645:Chickens; D016000:Cluster Analysis; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D004783:Environmental Microbiology; D005838:Genotype; D016133:Polymerase Chain Reaction; D011201:Poultry Diseases / Q000453:epidemiology / Q000382:microbiology*; D015995:Prevalence; D015363:Quinolones / Q000494:pharmacology; D012481:Salmonella Infections, Animal / Q000453:epidemiology / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D014774:Virulence / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015363:Quinolones