title: [Down-regulatory effect of Nucleostemin expression on signal molecule of PI3K/AKT/mTOR pathway in HL-60 cells].
abstract: This study was purpose to explore the down-regulatory effect of nucleostemin (NS) expression on signal molecules of PI3K/AKT/mTOR pathway belonged to candidate ways of p53-independent signal pathway in the leukemia cells. The expression of NS was interfered by using recombinant lentivirus expression vector NS-RNAi-GV248 to transfect HL-60 cells of p53 deficiency. The expression of NS and signal molecules of PI3K/AKT/mTOR pathway were detected by using Real-time PCR. The results of showed that the HL-60 cells were transfected by recombinant lentivirus vector NS-RNAi-GV248 successfully and with transfection rate up to 80%. According to results of Real-time PCR detection, the inhibition rate of NS gene was 56.5% in HL-60 cells. And the expression levels of PI3K,AKT and GβL mRNA (0.491 ± 0.084,0.398 ± 0.164, 0.472 ± 0.097 respectively) were obviously down-regulated by silencing NS, and showed statistical difference (P < 0.05) in comparison with control (1.002 ± 0.171, 1.000 ± 0.411, 1.001 ± 0.206 respectively) . It is concluded that the changes of signal molecules of PI3K/AKT/mTOR pathway positively correlate with NS down-regulation, which provides evidence for confirming PI3K/AKT/mTOR signal pathway possible as a type of NS p53-independent pathway.
authors.affiliation: Department of Laboratorial Medicine, The First Affiliated Hospital of Zhengzhou University, Faculty of Laboratorial Medicine, Zhengzhou Universities; Henan Province Key Subject of Clinical Medicine,Zhengzhou 450052, Henan Province, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Department of Laboratorial Medicine, The First Affiliated Hospital of Zhengzhou University, Faculty of Laboratorial Medicine, Zhengzhou Universities; Henan Province Key Subject of Clinical Medicine,Zhengzhou 450052, Henan Province, China.
authors.forename: Yuan-Yu
authors.initials: YY
authors.lastname: Wei
authors.affiliation: Department of Laboratorial Medicine, The First Affiliated Hospital of Zhengzhou University, Faculty of Laboratorial Medicine, Zhengzhou Universities; Henan Province Key Subject of Clinical Medicine,Zhengzhou 450052, Henan Province, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Laboratorial Medicine, The First Affiliated Hospital of Zhengzhou University, Faculty of Laboratorial Medicine, Zhengzhou Universities; Henan Province Key Subject of Clinical Medicine,Zhengzhou 450052, Henan Province, China.
authors.forename: Zhao-Bo
authors.initials: ZB
authors.lastname: Li
authors.affiliation: Department of Laboratorial Medicine, The First Affiliated Hospital of Zhengzhou University, Faculty of Laboratorial Medicine, Zhengzhou Universities; Henan Province Key Subject of Clinical Medicine,Zhengzhou 450052, Henan Province, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Laboratorial Medicine, The First Affiliated Hospital of Zhengzhou University, Faculty of Laboratorial Medicine, Zhengzhou Universities; Henan Province Key Subject of Clinical Medicine,Zhengzhou 450052, Henan Province, China. E-mail:ybh2002@163.com.
authors.forename: Bao-Hong
authors.initials: BH
authors.lastname: Yue
mesh_terms: D015536:Down-Regulation; D019204:GTP-Binding Proteins / Q000378:metabolism*; D018922:HL-60 Cells; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D012333:RNA, Messenger; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D019204:GTP-Binding Proteins; D009687:Nuclear Proteins; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D012333:RNA, Messenger; D058570:TOR Serine-Threonine Kinases; C469949:GNL3 protein, human; C546842:MTOR protein, human