title: Determination of sodium benzoate in food products by fluorescence polarization immunoassay.
abstract: A rapid and sensitive fluorescence polarization immunoassay (FPIA), based on a polyclonal antibody, has been developed for the detection of sodium benzoate in spiked samples. The immunogen and fluorescein-labeled analyte conjugate were successfully synthesized, and the tracer was purified by TLC. Under the optimal assay conditions, the FPIA shows a detection range of 0.3-20.0 μg mL(-1) for sodium benzoate with a detection limit of 0.26 μg mL(-1) in the borate buffer. In addition, the IC₅₀ value was 2.48 μg mL(-1), and the cross-reactivity of the antibodies with ten structurally and functionally related analogs were detected respectively. Four kinds of food samples (energy drink, candy, ice sucker, RIO(TM) cocktail) were selected to evaluate the application of FPIA in real systems. The recoveries were 96.68-106.55% in energy drink; 95.78-100.80% in candy, 86.97-102.70% in ice sucker, and 103.58-109.87% in benzoate contained sample RIO(TM) cocktail, and coefficients of variation of this method were all lower than 11.25%. Comparing with the detection results of HPLC, the developed FPIA has comparative performance in the real sample determination. The results suggest that the FPIA developed in this study is a rapid, convenient and simple method, which is suitable to be used as a screening tool for homogeneous detection of sodium benzoate in food products.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin 300071, China; Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300071, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin 300071, China; Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300071, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Meng
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin 300071, China; Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300071, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin 300071, China; Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300071, China.
authors.forename: Zhihuan
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow 119991, Russia.
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Eremin
authors.affiliation: Tianjin Sungene Biotech Co., Ltd., Tianjin 300450, China.
authors.forename: Junhong
authors.initials: J
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin 300071, China; Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300071, China. Electronic address: yinyongmei@nankai.edu.cn.
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Yin
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin 300071, China; Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300071, China. Electronic address: xirimo@nankai.edu.cn.
authors.forename: Rimo
authors.initials: R
authors.lastname: Xi
mesh_terms: D000906:Antibodies / Q000276:immunology; D003429:Cross Reactions; D016231:Fluorescence Polarization Immunoassay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D020160:Sodium Benzoate / Q000032:analysis*
chemical_list: D000906:Antibodies; D020160:Sodium Benzoate
keywords: Fluorescence polarization immunoassay; Food additives; Polyclonal antibody; Sodium benzoate