title: Current aspects in immunosensors.
abstract: Sensing applications can be used to report biomolecular interactions in order to elucidate the functions of molecules. The use of an analyte and a ligand is a common set-up in sensor development. For several decades, antibodies have been considered to be potential analytes or ligands for development of so-called "immunosensors." In an immunosensor, formation of the complex between antibody and antigen transduces the signal, which is measurable in various ways (e.g., both labeled and label-free based detection). Success of an immunosensor depends on various factors, including surface functionalization, antibody orientation, density of the antibody on the sensor platform, and configuration of the immunosensor. Careful optimization of these factors can generate clear-cut results for any immunosensor. Herein, current aspects, involved in the generated immunosensors, are discussed.
authors.affiliation: Advanced Medical & Dental Institute (AMDI), Universiti Sains Malaysia, 13200 Bertam, Kepala Batas, Penang, Malaysia; OCRCC, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address: gopis11@gmail.com.
authors.forename: Subash C B
authors.initials: SC
authors.lastname: Gopinath
authors.affiliation: Advanced Medical & Dental Institute (AMDI), Universiti Sains Malaysia, 13200 Bertam, Kepala Batas, Penang, Malaysia. Electronic address: tangth.amdi@usm.edu.my.
authors.forename: Thean-Hock
authors.initials: TH
authors.lastname: Tang
authors.affiliation: Advanced Medical & Dental Institute (AMDI), Universiti Sains Malaysia, 13200 Bertam, Kepala Batas, Penang, Malaysia.
authors.forename: Marimuthu
authors.initials: M
authors.lastname: Citartan
authors.affiliation: OCRCC, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Yeng
authors.initials: Y
authors.lastname: Chen
authors.affiliation: OCRCC, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Thangavel
authors.initials: T
authors.lastname: Lakshmipriya
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D055910:Antibodies, Immobilized / Q000737:chemistry* / Q000276:immunology; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000032:analysis* / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D008958:Models, Molecular; D019095:Point-of-Care Systems
chemical_list: D000906:Antibodies; D055910:Antibodies, Immobilized; D000941:Antigens
keywords: Antibody; Antigen; Immunoglobulin; Immunosensor