title: Autoimmune profiling with protein microarrays in clinical applications.
abstract: In recent years, knowledge about immune-related disorders has substantially increased, especially in the field of central nervous system (CNS) disorders. Recent innovations in protein-related microarray technology have enabled the analysis of interactions between numerous samples and up to 20,000 targets. Antibodies directed against ion channels, receptors and other synaptic proteins have been identified, and their causative roles in different disorders have been identified. Knowledge about immunological disorders is likely to expand further as more antibody targets are discovered. Therefore, protein microarrays may become an established tool for routine diagnostic procedures in the future. The identification of relevant target proteins requires the development of new strategies to handle and process vast quantities of data so that these data can be evaluated and correlated with relevant clinical issues, such as disease progression, clinical manifestations and prognostic factors. This review will mainly focus on new protein array technologies, which allow the processing of a large number of samples, and their various applications with a deeper insight into their potential use as diagnostic tools in neurodegenerative diseases and other diseases. This article is part of a Special Issue entitled: Biomarkers: A Proteomic Challenge.
authors.affiliation: Department of Medical Proteomics/Bioanalytics, Medizinisches Proteom-Center, Ruhr-Universität Bochum, 44801 Bochum, Germany.
authors.forename: Laura
authors.initials: L
authors.lastname: Abel
authors.affiliation: Department of Medical Proteomics/Bioanalytics, Medizinisches Proteom-Center, Ruhr-Universität Bochum, 44801 Bochum, Germany.
authors.forename: Simone
authors.initials: S
authors.lastname: Kutschki
authors.affiliation: Department of Medical Proteomics/Bioanalytics, Medizinisches Proteom-Center, Ruhr-Universität Bochum, 44801 Bochum, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Turewicz
authors.affiliation: Department of Medical Proteomics/Bioanalytics, Medizinisches Proteom-Center, Ruhr-Universität Bochum, 44801 Bochum, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Eisenacher
authors.affiliation: Department of Medical Proteomics/Bioanalytics, Medizinisches Proteom-Center, Ruhr-Universität Bochum, 44801 Bochum, Germany.
authors.forename: Jale
authors.initials: J
authors.lastname: Stoutjesdijk
authors.affiliation: Department of Medical Proteomics/Bioanalytics, Medizinisches Proteom-Center, Ruhr-Universität Bochum, 44801 Bochum, Germany; Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V., Dortmund, Germany.
authors.forename: Helmut E
authors.initials: HE
authors.lastname: Meyer
authors.affiliation: S. Josef Hospital, Ruhr-University Bochum, 44780 Bochum, Germany; St. Josef-Krankenhaus Kupferdreh, Heidbergweg 22-24, 45257 Essen, Germany.
authors.forename: Dirk
authors.initials: D
authors.lastname: Woitalla
authors.affiliation: Department of Medical Proteomics/Bioanalytics, Medizinisches Proteom-Center, Ruhr-Universität Bochum, 44801 Bochum, Germany. Electronic address: Caroline.May@rub.de.
authors.forename: Caroline
authors.initials: C
authors.lastname: May
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases / Q000175:diagnosis* / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis*; D006801:Humans; D040081:Protein Array Analysis / Q000379:methods*; D011506:Proteins / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*
chemical_list: D015415:Biomarkers; D011506:Proteins; D020543:Proteome
keywords: Autoantibody; Autoimmune disease; Protein microarray