title: Antioxidant, antibacterial, and cytoprotective activity of agathi leaf protein.
abstract: In the present study a protein termed agathi leaf protein (ALP) from Sesbania grandiflora Linn. (agathi) leaves was isolated after successive precipitation with 65% ammonium sulphate followed by purification on Sephadex G 75. The column chromatography of the crude protein resulted in four peaks of which Peak I (P I) showed maximum inhibition activity against hydroxyl radical. SDS-PAGE analysis of P I indicated that the molecular weight of the protein is ≈ 29 kDa. The purity of the protein was 98.4% as determined by RP-HPLC and showed a single peak with a retention time of 19.9 min. ALP was able to reduce oxidative damage by scavenging lipid peroxidation against erythrocyte ghost (85.50 ± 6.25%), linolenic acid (87.67 ± 3.14%) at 4.33  μ M, ABTS anion (88 ± 3.22%), and DNA damage (83 ± 4.20%) at 3.44  μ M in a dose-dependent manner. The purified protein offered significant protection to lymphocyte (72% at 30 min) induced damage by t-BOOH. In addition, ALP showed strong antibacterial activity against Pseudomonas aeruginosa (20 ± 3.64 mm) and Staphylococcus aureus (19 ± 1.53 mm) at 200  μ g/mL. The safety assessment showed that ALP does not induce cytotoxicity towards human lymphocyte at the tested concentration of 0.8 mg/mL.
authors.affiliation: Department of Studies in Microbiology, University of Mysore, Mysore 570006, India.
authors.forename: A S
authors.initials: AS
authors.lastname: Zarena
authors.affiliation: Department of Studies in Microbiology, University of Mysore, Mysore 570006, India.
authors.forename: Shubha
authors.initials: S
authors.lastname: Gopal
authors.affiliation: Department of Studies in Microbiology, University of Mysore, Mysore 570006, India.
authors.forename: R
authors.initials: R
authors.lastname: Vineeth