title: Induction of neoplastic cells in rat skin.
abstract: OBJECTIVE
  To investigate the induction of neoplastic lesions under the action of ultraviolet B radiation (UVR-B) and dimethyl benzanthracene (DMBA).
  
  
  METHODS
  Forty Wistar rats were assigned to four groups (ten animals each), according to the procedure: group A received UVR-B irradiation, group B received topic DMBA, group C, UVR-B+DMBA and group D as control, observed for ten weeks. In the tenth week they went through a skin biopsy and histopathological study. The average thickness of the epidermis was calculated and evaluated statistically.
  
  
  RESULTS
  Macroscopic lesions in group B were more of inflammatory kind compared to group A. Group C presented more injuries with neoplastic features than the others (p<0.01). Histologically there was a significant increase in thickness of the epidermis of all groups compared to control, however the greatest thickness measures occurred in Group C (p<0.01).
  
  
  CONCLUSIONS
  The population exposed to ultraviolet B radiation is subject to suffer skin lesions that can develop into cancer. The association with hydrocarbons as the dimethyl benzanthracene increases the possibility of malignancy. May not be clinically evident determine when a solar keratosis ends and when a CEC begins. For this reason, histological study associated with health education prompting the early and irreversible injury prevention is necessary.
authors.affiliation: Sao Paulo Federal University, PetrolinaPE, Brazil, Fellow Master degree, Postgraduate Program in Interdisciplinary Surgical Science, Sao Paulo Federal University (UNIFESP). Assistant Professor, Nursing Department, Sao Francisco Valley Federal University (UNIVASF), Petrolina-PE, Brazil. Intellectual and scientific content of the study, acquisition and interpretation of data.
authors.forename: Juliana Pedrosa
authors.initials: JP
authors.lastname: Korinfsky
authors.affiliation: UNIFESP, Paulista Medical School, Department of Surgery, Sao PauloSP, Brazil, Associate Professor, Department of Surgery, Paulista Medical School (EPM), UNIFESP, Sao Paulo-SP, Brazil. Conception, design, intellectual and scientific content of the study; critical revision.
authors.forename: Hélio
authors.initials: H
authors.lastname: Plapler
authors.affiliation: UNIVASF, Department of Medicine, PetrolinaPE, Brazil, Associate Professor, Department of Medicine, UNIVASF, Petrolina-PE, Brazil. Acquisition and interpretation of data.
authors.forename: Tania Rita
authors.initials: TR
authors.lastname: Moreno
authors.affiliation: UNIVASF, Department of Medicine, PetrolinaPE, Brazil, Associate Professor, Department of Medicine, UNIVASF, Petrolina-PE, Brazil. Acquisition and interpretation of data.
authors.forename: Itamar
authors.initials: I
authors.lastname: Santos
authors.affiliation: Oswaldo Cruz Foundation, Rio de JaneiroRJ, Brazil, PhD in Histology, Oswaldo Cruz Foundation (FioCRUZ), Rio de Janeiro-RJ, Brazil. Histological analysis, interpretation of data.
authors.forename: Celeste
authors.initials: C
authors.lastname: Maria
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D001706:Biopsy; D002273:Carcinogens; D004817:Epidermis / Q000473:pathology / Q000528:radiation effects*; D006838:Hydrocarbons; D007642:Keratosis / Q000209:etiology; D008297:Male; D009381:Neoplasms, Radiation-Induced / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D012878:Skin Neoplasms / Q000209:etiology* / Q000473:pathology; D013472:Sunlight / Q000009:adverse effects; D013997:Time Factors; D014466:Ultraviolet Rays
chemical_list: D002273:Carcinogens; D006838:Hydrocarbons; D015127:9,10-Dimethyl-1,2-benzanthracene