title: 3D tissue engineered supramolecular hydrogels for controlled chondrogenesis of human mesenchymal stem cells.
abstract: Despite a wide investigation of hydrogels as an artificial extracellular matrix, there are few scaffold systems for the facile spatiotemporal control of mesenchymal stem cells (MSCs). Here, we report 3D tissue engineered supramolecular hydrogels prepared with highly water-soluble monofunctionalized cucurbit[6]uril-hyaluronic acid (CB[6]-HA), diaminohexane conjugated HA (DAH-HA), and drug conjugated CB[6] (drug-CB[6]) for the controlled chondrogenesis of human mesenchymal stem cells (hMSCs). The mechanical property of supramolecular HA hydrogels was modulated by changing the cross-linking density for the spatial control of hMSCs. In addition, the differentiation of hMSCs was temporally controlled by changing the release profiles of transforming growth factor-β3 (TGF-β3) and/or dexamethasone (Dexa) from the hydrolyzable Dexa-CB[6]. The effective chondrogenic differentiation of hMSCs encapsulated in the monoCB[6]/DAH-HA hydrogel with TGF-β3 and Dexa-CB[6] was confirmed by biochemical glycosaminoglycan content analysis, real-time quantitative PCR, histological, and immunohistochemical analyses. Taken together, we could confirm the feasibility of cytocompatible monoCB[6]/DAH-HA hydrogels as a platform scaffold with controlled drug delivery for cartilage regeneration and other various tissue engineering applications.
authors.affiliation: School of Interdisciplinary Bioscience and Bioengineering, §Department of Materials Science and Engineering, and ∥Center for Self-Assembly and Complexity, Institute for Basic Science, Department of Chemistry, Division of Advanced Materials Science, Pohang University of Science and Technology (POSTECH) , San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk 790-784, Korea.
authors.forename: Hyuntae
authors.initials: H
authors.lastname: Jung
authors.forename: Ji Sun
authors.initials: JS
authors.lastname: Park
authors.forename: Junseok
authors.initials: J
authors.lastname: Yeom
authors.forename: Narayanan
authors.initials: N
authors.lastname: Selvapalam
authors.forename: Kyeng Min
authors.initials: KM
authors.lastname: Park
authors.forename: Kyunghoon
authors.initials: K
authors.lastname: Oh
authors.forename: Jeong-A
authors.initials: JA
authors.lastname: Yang
authors.forename: Keun Hong
authors.initials: KH
authors.lastname: Park
authors.forename: Sei Kwang
authors.initials: SK
authors.lastname: Hahn
authors.forename: Kimoon
authors.initials: K
authors.lastname: Kim
mesh_terms: D002356:Cartilage / Q000166:cytology; D002454:Cell Differentiation / Q000187:drug effects*; D020219:Chondrogenesis / Q000187:drug effects*; D005109:Extracellular Matrix / Q000737:chemistry; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry* / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D023822:Tissue Engineering
chemical_list: D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D006820:Hyaluronic Acid