title: Study of osteogenic differentiation of human adipose-derived stem cells (HASCs) on bacterial cellulose.
abstract: Bacterial cellulose (BC) has been proposed as a biomaterial applied in biomedical scope due to its good biocompatibility. Recent reports showed that human adipose-derived stem cells (HASCs) have become a new choice to be used as seeding cells in tissue engineering. The objective of this study is to explore the potential of using BC and HASCs as scaffold and seeding cells in bone tissue engineering. The osteogenic differentiation was investigated by Von Kossa, Alizarin Red, ALP cellular staining and RT-PCR. The results showed that HASCs took a successful osteogenic differentiation on BC. Moreover, the in vivo animal test also provided the confirmation of the repair ability of BC on damaged bone. In conclusion, the author demonstrates the osteogenic differentiation of HASCs on BC and the feasibility of using BC and HASCs as scaffold and seeding cells in bone tissue engineering.
authors.affiliation: Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Zang
authors.affiliation: Department of Orthopaedic Surgery, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhuo
authors.affiliation: Department of Orthopaedics, Peking Union Medical College Hospital, Beijing 100730, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Chang
authors.affiliation: Department of Orthopaedics, Peking Union Medical College Hospital, Beijing 100730, China.
authors.forename: Guixing
authors.initials: G
authors.lastname: Qiu
authors.affiliation: Department of Orthopaedics, Peking Union Medical College Hospital, Beijing 100730, China. Electronic address: wuzh3000@126.com.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China. Electronic address: yang_sunny@yahoo.com.
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
mesh_terms: D000091:Acetobacter / Q000737:chemistry; D000273:Adipose Tissue / Q000166:cytology; D053687:Adult Stem Cells / Q000166:cytology* / Q000187:drug effects; D000818:Animals; D002482:Cellulose / Q000494:pharmacology*; D006801:Humans; D010012:Osteogenesis; D011817:Rabbits; D033581:Stem Cell Transplantation; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D002482:Cellulose
keywords: Artificial scaffold; Bacterial cellulose; Biomaterials; Bone tissue engineering; Human adipose-derived stem cells