title: The role of endoglucanase and endoxylanase in liquefaction of hydrothermally pretreated wheat straw.
abstract: The role of endocellulases and endoxylanase during liquefaction and saccharification of hydrothermally pretreated wheat straw was studied. The use of a flow-loop setup with in-line magnetic resonance imaging enabled frequent measurements of viscosity at 55°C during saccharification at 6% total solids content. Viscosity data were complemented with off-line measurements of fiber lengths and release of soluble sugars. A clear correlation between fiber attrition and a decrease in viscosity was found. Fiber lengths and viscosity dropped quickly within the first hour and then stagnated, while sugar yields increased substantially thereafter, illustrating that liquefaction and saccharification are separate mechanisms. Both endoglucanase and endoxylanase were shown to have a significant effect on viscosity during liquefaction while the addition of endoxylanase also increased sugar yield.
authors.affiliation: University of Copenhagen, Faculty of Science, 1958 Frederiksberg C, Copenhagen, Denmark.
authors.forename: Pernille Anastasia
authors.initials: PA
authors.lastname: Skovgaard
authors.forename: Lisbeth Garbrecht
authors.initials: LG
authors.lastname: Thygesen
authors.forename: Henning
authors.initials: H
authors.lastname: Jørgensen
authors.forename: Maria
authors.initials: M
authors.lastname: Cardona
authors.forename: Emilio
authors.initials: E
authors.lastname: Tozzi
authors.forename: Michael
authors.initials: M
authors.lastname: McCarthy
authors.forename: Matti
authors.initials: M
authors.lastname: Siika-Aho
authors.forename: Tina
authors.initials: T
authors.lastname: Jeoh
mesh_terms: D002241:Carbohydrates / Q000737:chemistry; D002480:Cellulase / Q000737:chemistry*; D044602:Cellulases / Q000737:chemistry*; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry*; D005285:Fermentation; D006868:Hydrolysis; D008279:Magnetic Resonance Imaging; D014908:Triticum / Q000737:chemistry*; D014783:Viscosity
chemical_list: D002241:Carbohydrates; D044602:Cellulases; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases
keywords: endoglucanase; endoxylanase; fiber attrition; magnetic resonance imaging; thermostable enzymes; viscosity