title: Influence of somatic cell donor breed on reproductive performance and comparison of prenatal growth in cloned canines.
abstract: Using in vivo-flushed oocytes from a homogenous dog population and subsequent embryo transfer after nuclear transfer, we studied the effects of donor cells collected from 10 different breeds on cloning efficiency and perinatal development of resulted cloned puppies. The breeds were categorized into four groups according to their body weight: small (≤9 kg), medium (>9-20 kg), large (>20-40 kg), and ultra large (>40 kg). A total of 1611 cloned embryos were transferred into 454 surrogate bitches for production of cloned puppies. No statistically significant differences were observed for initial pregnancy rates at Day 30 of embryo transfer for the donor cells originated from different breeds. However, full-term pregnancy rates were 16.5%, 11.0%, 10.0%, and 7.1% for the donor cells originated from ultra-large breed, large, medium, and small breeds, respectively, where pregnancy rate in the ultra-large group was significantly higher compared with the small breeds (P < 0.01). Perinatal mortality until weaning was significantly higher in small breeds (33.3%) compared with medium, large, or ultra-large breeds where no mortality was observed. The mean birth weight of cloned pups significantly increased proportional to breed size. The highest litter size was examined in ultra-large breeds. There was no correlation between the number of embryo transferred and litter size. Taken together, the efficiency of somatic cell cloning and fetal survival after embryo transfer may be affected significantly by selecting the appropriate genotype.
authors.affiliation: Sooam Biotech Research Foundation, Seoul, Republic of Korea; Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
authors.forename: Yeon Woo
authors.initials: YW
authors.lastname: Jeong
authors.affiliation: Sooam Biotech Research Foundation, Seoul, Republic of Korea.
authors.forename: Joung Joo
authors.initials: JJ
authors.lastname: Kim
authors.affiliation: Sooam Biotech Research Foundation, Seoul, Republic of Korea.
authors.forename: Mohammad Shamim
authors.initials: MS
authors.lastname: Hossein
authors.affiliation: Sooam Biotech Research Foundation, Seoul, Republic of Korea.
authors.forename: Kyu Chan
authors.initials: KC
authors.lastname: Hwang
authors.affiliation: Sooam Biotech Research Foundation, Seoul, Republic of Korea.
authors.forename: In-sung
authors.initials: IS
authors.lastname: Hwang
authors.affiliation: Sooam Biotech Research Foundation, Seoul, Republic of Korea; Laboratory of Veterinary Embryology and Biotechnology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea.
authors.forename: Sang Hwan
authors.initials: SH
authors.lastname: Hyun
authors.affiliation: Department of Animal Sciences, Chungbuk National University, Cheongju, Republic of Korea.
authors.forename: Nam-Hyung
authors.initials: NH
authors.lastname: Kim
authors.affiliation: Department of Veterinary Physiology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Republic of Korea.
authors.forename: Ho Jae
authors.initials: HJ
authors.lastname: Han
authors.affiliation: Sooam Biotech Research Foundation, Seoul, Republic of Korea. Electronic address: hwangws@sooam.org.
authors.forename: Woo Suk
authors.initials: WS
authors.lastname: Hwang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001835:Body Weight; D019976:Cloning, Organism / Q000662:veterinary*; D004285:Dogs / Q000235:genetics* / Q000502:physiology*; D004624:Embryo Transfer / Q000662:veterinary; D004622:Embryo, Mammalian / Q000166:cytology / Q000196:embryology; D005260:Female; D005347:Fibroblasts; D005838:Genotype; D008297:Male; D011247:Pregnancy
keywords: Breed; Canine; Donor cell; Prenatal; Somatic cell nuclear transfer