title: MicroRNA-127-3p promotes glioblastoma cell migration and invasion by targeting the tumor-suppressor gene SEPT7.
abstract: MicroRNAs (miRNAs) are small non-coding RNAs of 20-25 nucleotides in length that are capable of modulating gene expression post-transcriptionally. The potential roles of miRNAs in the tumorigenesis of glioblastoma (GBM) have been under intensive studies in the past few years. In the present study, we found a positive correlation between the levels of miR-127-3p and the cell migration and invasion abilities in several human GBM cell lines. We showed that miR-127-3p promoted cell migration and invasion of GBM cells using in vitro cell lines and in vivo mouse models. We identified SEPT7, a known tumor-suppressor gene that has been reported to suppress GBM cell migration and invasion, as a direct target of miR-127-3p. SEPT7 was able to partially abrogate the effect of miR-127-3p on cell migration and invasion. In addition, microarray analysis revealed that miR-127-3p regulated a number of migration and invasion-related genes. Finally, we verified that miR-127-3p affected the remodeling of the actin cytoskeleton mediated by SEPT7 in GBM cells.
authors.affiliation: Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education), The Second Afﬁliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, P.R. China.
authors.forename: Huawei
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Systems Biology Division, Zhejiang-California International Nanosystems Institute, Zhejiang University, Hangzhou, Zhejiang 310029, P.R. China.
authors.forename: Dasong
authors.initials: D
authors.lastname: Hua
authors.affiliation: Systems Biology Division, Zhejiang-California International Nanosystems Institute, Zhejiang University, Hangzhou, Zhejiang 310029, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery and Brain Tumor Research Laboratory, Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Lan
authors.affiliation: Department of Neurosurgery and Brain Tumor Research Laboratory, Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Swedish Neuroscience Institute, Swedish Medical Center, Seattle, WA 98122, USA.
authors.forename: Jae-Geun
authors.initials: JG
authors.lastname: Yoon
authors.affiliation: Systems Biology Division, Zhejiang-California International Nanosystems Institute, Zhejiang University, Hangzhou, Zhejiang 310029, P.R. China.
authors.forename: Xu
authors.initials: X
authors.lastname: Han
authors.affiliation: Systems Biology Division, Zhejiang-California International Nanosystems Institute, Zhejiang University, Hangzhou, Zhejiang 310029, P.R. China.
authors.forename: Lisha
authors.initials: L
authors.lastname: Li
authors.affiliation: Swedish Neuroscience Institute, Swedish Medical Center, Seattle, WA 98122, USA.
authors.forename: Gregory
authors.initials: G
authors.lastname: Foltz
authors.affiliation: Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education), The Second Afﬁliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, P.R. China.
authors.forename: Shu
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education), The Second Afﬁliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, P.R. China.
authors.forename: Biaoyang
authors.initials: B
authors.lastname: Lin
mesh_terms: D008841:Actin Cytoskeleton / Q000235:genetics / Q000378:metabolism; D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000473:pathology*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D058112:Septins / Q000235:genetics*
chemical_list: D018797:Cell Cycle Proteins; C547276:MIRN127 microRNA, human; D035683:MicroRNAs; C113158:SEPTIN7 protein, human; D058112:Septins