title: Transcriptome analysis of differentially expressed genes relevant to variegation in peach flowers.
abstract: BACKGROUND
  Variegation in flower color is commonly observed in many plant species and also occurs on ornamental peaches (Prunus persica f. versicolor [Sieb.] Voss). Variegated plants are highly valuable in the floricultural market. To gain a global perspective on genes differentially expressed in variegated peach flowers, we performed large-scale transcriptome sequencing of white and red petals separately collected from a variegated peach tree.
  
  
  RESULTS
  A total of 1,556,597 high-quality reads were obtained, with an average read length of 445 bp. The ESTs were assembled into 16,530 contigs and 42,050 singletons. The resulting unigenes covered about 60% of total predicted genes in the peach genome. These unigenes were further subjected to functional annotation and biochemical pathway analysis. Digital expression analysis identified a total of 514 genes differentially expressed between red and white flower petals. Since peach flower coloration is determined by the expression and regulation of structural genes relevant to flavonoid biosynthesis, a detailed examination detected four key structural genes, including C4H, CHS, CHI and F3H, expressed at a significantly higher level in red than in white petal. Except for the structural genes, we also detected 11 differentially expressed regulatory genes relating to flavonoid biosynthesis. Using the differentially expressed structural genes as the test objects, we validated the digital expression results by using quantitative real-time PCR, and the differential expression of C4H, CHS and F3H were confirmed.
  
  
  CONCLUSIONS
  In this study, we generated a large EST collection from flower petals of a variegated peach. By digital expression analysis, we identified an informative list of candidate genes associated with variegation in peach flowers, which offered a unique opportunity to uncover the genetic mechanisms underlying flower color variegation.
authors.affiliation: The Southern Modern Forestry Collaborative Innovation Center, Nanjing Forestry University, Nanjing, Jiangsu, China.
authors.forename: Yingnan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: The Southern Modern Forestry Collaborative Innovation Center, Nanjing Forestry University, Nanjing, Jiangsu, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Mao
authors.affiliation: The Southern Modern Forestry Collaborative Innovation Center, Nanjing Forestry University, Nanjing, Jiangsu, China.
authors.forename: Hailin
authors.initials: H
authors.lastname: Liu
authors.affiliation: Institute of Biology and Resources, Jiangxi Academy of Sciences, Nanchang, Jiangxi, China.
authors.forename: Faxin
authors.initials: F
authors.lastname: Yu
authors.affiliation: The Southern Modern Forestry Collaborative Innovation Center, Nanjing Forestry University, Nanjing, Jiangsu, China.
authors.forename: Shuxian
authors.initials: S
authors.lastname: Li
authors.affiliation: The Southern Modern Forestry Collaborative Innovation Center, Nanjing Forestry University, Nanjing, Jiangsu, China.
authors.forename: Tongming
authors.initials: T
authors.lastname: Yin
mesh_terms: D002874:Chromosome Mapping; D020224:Expressed Sequence Tags; D035264:Flowers / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D017343:Genes, Plant; D058977:Molecular Sequence Annotation; D010858:Pigmentation / Q000235:genetics; D027861:Prunus / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome