title: Nanostructured polymeric coatings based on chitosan and dopamine-modified hyaluronic acid for biomedical applications.
abstract: In a marine environment, specific proteins are secreted by mussels and used as a bioglue to stick to a surface. These mussel proteins present an unusual amino acid 3,4-dihydroxyphenylalanine (known as DOPA). The outstanding adhesive properties of these materials in the sea harsh conditions have been attributed to the presence of the catechol groups present in DOPA. Inspired by the structure and composition of these adhesive proteins, dopamine-modified hyaluronic acid (HA-DN) prepared by carbodiimide chemistry is used to form thin and surface-adherent dopamine films. This conjugate was characterized by distinct techniques, such as nuclear magnetic resonance and ultraviolet spectrophotometry. Multilayer films are developed based on chitosan and HA-DN to form polymeric coatings using the layer-by-layer methodology. The nanostructured films formation is monitored by quartz crystal microbalance. The film surface is characterized by atomic force microscopy and scanning electron microscopy. Water contact angle measurements are also conducted. The adhesion properties are analyzed showing that the nanostructured films with dopamine promote an improved adhesion. In vitro tests show an enhanced cell adhesion, proliferation and viability for the biomimetic films with catechol groups, demonstrating their potential to be used in distinct biomedical applications.
authors.affiliation: 3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, AvePark, 4806-90, Taipas, Guimarães, Portugal; ICVS/3B's PT Government Associate Laboratory, Braga/Guimarães, Portugal.
authors.forename: Ana I
authors.initials: AI
authors.lastname: Neto
authors.forename: Ana C
authors.initials: AC
authors.lastname: Cibrão
authors.forename: Clara R
authors.initials: CR
authors.lastname: Correia
authors.forename: Rita R
authors.initials: RR
authors.lastname: Carvalho
authors.forename: Gisela M
authors.initials: GM
authors.lastname: Luz
authors.forename: Gloria G
authors.initials: GG
authors.lastname: Ferrer
authors.forename: Gabriela
authors.initials: G
authors.lastname: Botelho
authors.forename: Catherine
authors.initials: C
authors.lastname: Picart
authors.forename: Natália M
authors.initials: NM
authors.lastname: Alves
authors.forename: João F
authors.initials: JF
authors.lastname: Mano
mesh_terms: D000818:Animals; D032701:Biomimetics; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D004298:Dopamine / Q000737:chemistry*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D008422:Materials Testing; D008567:Membranes, Artificial; D051379:Mice; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D058525:Quartz Crystal Microbalance Techniques; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D020099:Coated Materials, Biocompatible; D008567:Membranes, Artificial; D011108:Polymers; D006820:Hyaluronic Acid; D048271:Chitosan; D004298:Dopamine
keywords: adhesion; dopamine; marine mussels; multilayer films; surface modification