title: Role of miR-191/425 cluster in tumorigenesis and diagnosis of gastric cancer.
abstract: Gastric cancer (GC) is among the most frequent types of cancer worldwide. Therefore, understanding the biology of GC tumorigenesis is important for appropriate diagnosis and patient surveillance. The miR-191/425 cluster has been reported to be overexpressed in various human cancers, but the tumorigenic role and clinical significance of miR-191/425 overexpression in gastric carcinogenesis is currently undefined. In this study, the expression of miR-191 and miR-425 in GC tissue and serum was assessed, and the relationship between miRNA expression and clinicopathological data was analyzed. We found that miR-191 and miR-425 were both significantly increased in human GC tissues relative to adjacent normal controls. In addition, miR-191 levels correlated with GC tumor stage and metastatic state. Furthermore, the level of serum miR-191 was significantly higher in the GC group than in the control group when using serum miR-16 as an endogenous control. Finally, inhibition of miR-191 or miR-425 in the GC cell lines HGC-27 not only reduced cell proliferation and cell cycle progression but also impaired cell migration and invasion. Taken together, our results revealed the oncogenic roles of miR-191 and miR-425 in gastric carcinogenesis, and indicated the potential use of serum miR-191 as a novel and stable biomarker for GC diagnosis.
authors.affiliation: Department of General Surgery, China-Japan Friendship Hospital, Beijing 100029, China. pwz_75@163.com.
authors.forename: Wei-Zhao
authors.initials: WZ
authors.lastname: Peng
authors.affiliation: Department of General Surgery, China-Japan Friendship Hospital, Beijing 100029, China. maren517@sohu.com.
authors.forename: Ren
authors.initials: R
authors.lastname: Ma
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China. wo_wfang@hotmail.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China. j-yu@ibms.pumc.edu.cn.
authors.forename: Jia
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of General Surgery, China-Japan Friendship Hospital, Beijing 100029, China. l_zhibin@hotmail.com.
authors.forename: Zhi-Bin
authors.initials: ZB
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics; D063646:Carcinogenesis / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D005810:Multigene Family; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013270:Stomach / Q000473:pathology; D013274:Stomach Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; C546177:MIRN191 microRNA, human; C555677:MIRN425 microRNA, human; D035683:MicroRNAs