title: Inhibition of SMP30 gene expression influences the biological characteristics of human Hep G2 cells.
abstract: Senescence marker protein 30 (SMP30), a hepatocellular carcinoma (HCC) associated antigen had been identified by our research group. To study its mechanisms of regulation and associations with the occurrence and development of HCC, we inhibited expression by RNAi technique, and observed effects on the biological characteristics of Hep G2 cells. In cell viability assays, cell growth in the experimental group (with siRNA transfection) was elevated. In Transwell invasion assays, compared with blank and control groups, numbers of invading cells in the experimental group were significantly increased, whereas in apoptosis assays, the percentage apoptosis demonstrated no differences, but after UV irradiation, that in the experimental group was higher than the other two groups. In a word, SMP30 can inhibit the proliferation and invasion of human hepatoma cells and thus can be regarded as a cancer suppressive factor.
authors.affiliation: Anatomy Teaching and Research Section, Guangxi Traditional Chinese Medical University, Nanning, China E-mail : zhsh8000@163.com.
authors.forename: Sheng-Chang
authors.initials: SC
authors.lastname: Zhang
authors.forename: Ming-Kang
authors.initials: MK
authors.lastname: Liang
authors.forename: Guang-Lin
authors.initials: GL
authors.lastname: Huang
authors.forename: Kui
authors.initials: K
authors.lastname: Jiang
authors.forename: Su-Fang
authors.initials: SF
authors.lastname: Zhou
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhao
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics*; D017209:Apoptosis; D002135:Calcium-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics; D015870:Gene Expression; D056945:Hep G2 Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000096:biosynthesis / Q000235:genetics*; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D009361:Neoplasm Invasiveness / Q000235:genetics; D034622:RNA Interference; D034741:RNA, Small Interfering
chemical_list: D000951:Antigens, Neoplasm; D002135:Calcium-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; C513323:RGN protein, human; D034741:RNA, Small Interfering