title: Nutraceutical potential of tinctures from fruits, green husks, and leaves of Juglans regia L.
abstract: The aim of this study was to assess the phenolic composition and nutraceutical potential of tinctures from fruits in two stages of maturity (F3, younger; F25, older), green husks (GH), and leaves (L) of Juglans regia L. In all extracts gallic, protocatechuic, 3-caffeoylquinic, 3-ρ-coumaroylquinic, 4-caffeoylquinic, 4-ρ-coumaroylquinic, and ρ -coumaric acids and quercetin-3-O-deoxyhexoside were detected using UPLC-MS technique. Caffeic acid hexoside I and quercetin-3-O-deoxyhexoside I have been identified in GH tincture. The highest ability to chelate Fe(2+) was observed for GH tincture (EC50 = 71.01 ± 3.55 mg FM/mL), whereas the lowest was observed (EC50 = 131.06 ± 6.55 mg FM/mL) for F3 tincture. The highest reducing power was found for F3 and F25 (EC50 = 32.47 ± 1.53 and 36.07 ± 1.72 mg FM/mL, resp.). Ability of tinctures to prevent lipids against oxidation was relatively low. The highest activity (EC50 = 126.49 ± 6.32 mg FM/mL) was determined for F25. Tested tinctures showed relatively high antiradical activity-EC50 values ranged from 100.56 ± 5.03 to 129.04 ± 6.45 mg FM/mL for L and F25, respectively. The results obtained suggest that J. regia can be a source of bioactive compounds with antioxidant properties.
authors.affiliation: Department of Biochemistry and Food Chemistry, University of Life Sciences, Skromna Street 8, 20-704 Lublin, Poland.
authors.forename: Urszula
authors.identifier: 0000-0002-7308-4335
authors.initials: U
authors.lastname: Gawlik-Dziki
authors.affiliation: Department of Biochemistry and Food Chemistry, University of Life Sciences, Skromna Street 8, 20-704 Lublin, Poland.
authors.forename: Agata
authors.initials: A
authors.lastname: Durak
authors.affiliation: Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute, Czartoryskich Street 8, 24-100 Pulawy, Poland.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Pecio
authors.affiliation: Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute, Czartoryskich Street 8, 24-100 Pulawy, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Kowalska
mesh_terms: D002853:Chromatography, Liquid; D019587:Dietary Supplements; D031324:Juglans / Q000737:chemistry*; D010936:Plant Extracts / Q000627:therapeutic use*; D018514:Plant Structures / Q000737:chemistry*
chemical_list: D010936:Plant Extracts