title: Intratumoral injection of BCG-CWS-pretreated dendritic cells following tumor cryoablation.
abstract: Intratumoral administration of dendritic cells (DC) following cryoablation of tumor is one of the personalized cancer immunotherapies which is able to induce immune responses to multiple endogenous tumor antigens, including shared and unique antigens. Here we describe protocols of cryoablation of tumors, generation of cultured DC, pretreatment of DC with a Toll-like receptor (TLR)-stimulating purified component of Bacillus Calmette-Guerin cell wall fraction (BCG-CWS) and highly immunogenic keyhole limpet hemocyanin (KLH) antigen, and combined use of tumor cryoablation and intratumoral administration of BCG-CWS-pretreated DC in both a murine model and cancer patients.
authors.affiliation: Division of Cellular Signaling, Institute for Advanced Medical Research, Keio University School of Medicine, Tokyo, Japan.
authors.forename: Naoshi
authors.initials: N
authors.lastname: Kawamura
authors.forename: Masaru
authors.initials: M
authors.lastname: Udagawa
authors.forename: Tomonobu
authors.initials: T
authors.lastname: Fujita
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Sakurai
authors.forename: Tomonori
authors.initials: T
authors.lastname: Yaguchi
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kawakami
mesh_terms: D055011:Ablation Techniques; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002458:Cell Fractionation; D045744:Cell Line, Tumor; D002473:Cell Wall / Q000276:immunology*; D003131:Combined Modality Therapy; D003452:Cryosurgery; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology*; D005260:Female; D006801:Humans; D007167:Immunotherapy; D015552:Injections, Intralesional; D051379:Mice; D009163:Mycobacterium bovis / Q000166:cytology*; D009369:Neoplasms / Q000276:immunology / Q000473:pathology / Q000601:surgery / Q000628:therapy*