title: Transcriptional mapping of the messenger and leader RNAs of orchid fleck virus, a bisegmented negative-strand RNA virus.
abstract: The transcriptional strategy of orchid fleck virus (OFV), which has a two-segmented negative-strand RNA genome and resembles plant nucleorhabdoviruses, remains unexplored. In this study, the transcripts of six genes encoded by OFV RNA1 and RNA2 in the poly(A)-enriched RNA fraction from infected plants were molecularly characterized. All of the OFV mRNAs were initiated at a start sequence 3'-UU-5' with one to three non-viral adenine nucleotides which were added at the 5' end of each mRNA, whereas their 3' termini ended with a 5'-AUUUAAA(U/G)AAAA(A)n-3' sequence. We also identified the presence of polyadenylated short transcripts derived from the 3'-terminal leader regions of both genomic and antigenomic strands, providing the first example of plus- and minus-strand leader RNAs in a segmented minus-strand RNA virus. The similarity in the transcriptional strategy between this bipartite OFV and monopartite rhabdoviruses, especially nucleorhabdoviruses (family Rhabdoviridae) is additional support for their close relationship.
authors.affiliation: Institute of Plant Science and Resources (IPSR), Okayama University, Kurashiki 710-0046, Japan. Electronic address: hkondo@rib.okayama.u-ac.jp.
authors.forename: Hideki
authors.initials: H
authors.lastname: Kondo
authors.affiliation: Institute of Plant Science and Resources (IPSR), Okayama University, Kurashiki 710-0046, Japan.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Maruyama
authors.affiliation: Institute of Plant Science and Resources (IPSR), Okayama University, Kurashiki 710-0046, Japan.
authors.forename: Sotaro
authors.initials: S
authors.lastname: Chiba
authors.affiliation: Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.
authors.forename: Ida Bagus
authors.initials: IB
authors.lastname: Andika
authors.affiliation: Institute of Plant Science and Resources (IPSR), Okayama University, Kurashiki 710-0046, Japan.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Suzuki
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012328:RNA Viruses / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D020506:Untranslated Regions
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral; D020506:Untranslated Regions
keywords: Bipartite genome; Dichorhavirus; Gene junction; Leader RNA; Negative-strand RNA virus; Orchid fleck virus; Polyadenylated; RACE; Rhabdovirus; Transcription