title: Intragenus generalized transduction in Staphylococcus spp. by a novel giant phage.
abstract: Bacteriophage (phage)-mediated generalized transduction is expected to contribute to the emergence of drug-resistant staphylococcal clones in various environments. In this study, novel phage S6 was isolated from sewage and used to test generalized transduction in human- and animal-derived staphylococci. Phage S6 was a novel type of giant myophage, which possessed a DNA genome that contained uracil instead of thymine, and it could infect all of the tested staphylococcal species. The phage S6 appeared to be similar to the transducing phage PBS1, which infects Bacillus spp. Moreover, phage S6 facilitated the transduction of a plasmid in Staphylococcus aureus and from S. aureus to non-aureus staphylococcal species, as well as vice versa. Transduction of methicillin resistance also occurred in S. aureus. This is the first report of successful intragenus generalized transduction among staphylococci.
authors.affiliation: 1] Department of Microbiology and Infection, Faculty of Medicine, Kochi University, Kochi, Japan [2] Center for Innovative and Translational Medicine, Faculty of Medicine, Kochi University, Kochi, Japan.
authors.forename: Jumpei
authors.initials: J
authors.lastname: Uchiyama
authors.affiliation: Department of Microbiology and Infection, Faculty of Medicine, Kochi University, Kochi, Japan.
authors.forename: Iyo
authors.initials: I
authors.lastname: Takemura-Uchiyama
authors.affiliation: Interdisciplinary Research Organization, University of Miyazaki, Miyazaki, Japan.
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Sakaguchi
authors.affiliation: Research and Education Faculty, Multidisciplinary Science Cluster, Interdisciplinary Science Unit, Kochi University, Kochi, Japan.
authors.forename: Keiji
authors.initials: K
authors.lastname: Gamoh
authors.affiliation: Research Institute of Molecular Genetics, Kochi University, Kochi, Japan.
authors.forename: Shin-ichiro
authors.initials: S
authors.lastname: Kato
authors.affiliation: 1] Department of Microbiology and Infection, Faculty of Medicine, Kochi University, Kochi, Japan [2] Center for Innovative and Translational Medicine, Faculty of Medicine, Kochi University, Kochi, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Daibata
authors.affiliation: Science Research Center, Kochi University, Kochi, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Ujihara
authors.affiliation: Laboratory of Veterinary Public Health, Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
authors.forename: Naoaki
authors.initials: N
authors.lastname: Misawa
authors.affiliation: 1] Department of Microbiology and Infection, Faculty of Medicine, Kochi University, Kochi, Japan [2] Center for Innovative and Translational Medicine, Faculty of Medicine, Kochi University, Kochi, Japan.
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Matsuzaki
mesh_terms: D000818:Animals; D006801:Humans; D016106:Methicillin Resistance / Q000235:genetics*; D013210:Staphylococcus / Q000235:genetics; D013204:Staphylococcus Phages / Q000235:genetics* / Q000648:ultrastructure; D013211:Staphylococcus aureus / Q000235:genetics / Q000821:virology; D014161:Transduction, Genetic