title: Worldwide dissemination of acquired carbapenem-hydrolysing class D β-lactamases in Acinetobacter spp. other than Acinetobacter baumannii.
abstract: The aim of this study was to identify acquired OXA-type carbapenemases in Acinetobacter spp. other than Acinetobacter baumannii. From a total of 453 carbapenem-susceptible and -resistant Acinetobacter isolates collected worldwide, 23 were positive for blaOXA genes by multiplex PCR. These isolates were identified as Acinetobacter pittii (n=18), Acinetobacter nosocomialis (n=2), Acinetobacter junii (n=1) and Acinetobacter genomic species 14TU/13BJ (n=2). The blaOXA genes and associated insertion sequence (IS) elements were sequenced by primer walking. In 11 of these isolates, sequencing of the PCR products revealed that they were false-positive for blaOXA. The remaining 12 isolates, originating from Europe, Asia, South America, North America and South Africa, harboured OXA-23 (n=4), OXA-58 (n=5), OXA-40-like (n=1) and OXA-143-like (n=1); one A. pittii isolate harboured both OXA-23 and OXA-58. IS elements were associated with blaOXA in 10 isolates. OXA multiplex PCR showed a high degree of false-positive results (47.8%), indicating that detection of blaOXA in non-baumanniiAcinetobacter spp. should be confirmed using additional methods.
authors.affiliation: Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Goldenfelsstr. 19-21, 50935 Cologne, Germany.
authors.forename: Esther
authors.initials: E
authors.lastname: Zander
authors.affiliation: Servizo de Microbioloxía-INIBIC, Complexo Hospitalario Universitario A Coruña, As Xubias s/n, 15006 A Coruña, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Fernández-González
authors.affiliation: Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Goldenfelsstr. 19-21, 50935 Cologne, Germany.
authors.forename: Xenia
authors.initials: X
authors.lastname: Schleicher
authors.affiliation: Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Goldenfelsstr. 19-21, 50935 Cologne, Germany.
authors.forename: Cathrin
authors.initials: C
authors.lastname: Dammhayn
authors.affiliation: University of Queensland Centre for Clinical Research, The University of Queensland, Brisbane, Queensland, Australia; Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Witchuda
authors.initials: W
authors.lastname: Kamolvit
authors.affiliation: Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Goldenfelsstr. 19-21, 50935 Cologne, Germany.
authors.forename: Harald
authors.initials: H
authors.lastname: Seifert
authors.affiliation: Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Goldenfelsstr. 19-21, 50935 Cologne, Germany. Electronic address: paul.higgins@uni-koeln.de.
authors.forename: Paul G
authors.initials: PG
authors.lastname: Higgins
mesh_terms: D000150:Acinetobacter / Q000145:classification / Q000187:drug effects* / Q000235:genetics*; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology; D001483:Base Sequence; D015780:Carbapenems / Q000378:metabolism / Q000494:pharmacology*; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D008826:Microbial Sensitivity Tests; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D001618:beta-Lactamases
keywords: Carbapenemase; OXA multiplex PCR; Susceptibility