title: Description of Lutimonas halocynthiae sp. nov., isolated from a golden sea squirt (Halocynthia aurantium), reclassification of Aestuariicola saemankumensis as Lutimonas saemankumensis comb. nov. and emended description of the genus Lutimonas.
abstract: A Gram-stain-negative, non-motile, coccoid, ovoid or rod-shaped bacterial strain, designated RSS3-C1(T), was isolated from a golden sea squirt (Halocynthia aurantium) collected from the East Sea, South Korea. Strain RSS3-C1(T) was found to grow optimally at 20-25 °C, at pH 7.0-8.0 and in the presence of 2.0% (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain RSS3-C1(T) clustered with the type strains of Lutimonas vermicola and Aestuariicola saemankumensis. Strain RSS3-C1(T) exhibited 98.8% 16S rRNA gene sequence similarity to each type strain. Strain RSS3-C1(T) contained MK-6 as the predominant menaquinone and iso-C(15 : 0), iso-C(17 : 0) 3-OH and anteiso-C(15 : 0) as the major fatty acids. The major polar lipids of strain RSS3-C1(T) were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain RSS3-C1(T) was 39.2 mol%, and DNA-DNA relatedness to the type strains of and was 21±5.3 and 26±7.5 %, respectively. The differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain RSS3-C1(T) is separated from and . On the basis of the data presented, strain RSS3-C1(T) is considered to represent a novel species of the genus Lutimonas, for which the name Lutimonas halocynthiae sp. nov. is proposed. The type strain is RSS3-C1(T) ( = KCTC 32537(T) = CECT 8444(T)). In this study, it is also proposed that Aestuariicola saemankumensis should be reclassified as a member of the genus Lutimonas, as Lutimonas saemankumensis comb. nov. (type strain SMK-142(T) = KCTC 22171(T) = CCUG 55329(T)), and the description of the genus Lutimonas is emended.
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute (NFRDI), Gijang, Busan 619-705, South Korea.
authors.forename: Young-Ok
authors.initials: YO
authors.lastname: Kim
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Park
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute (NFRDI), Gijang, Busan 619-705, South Korea.
authors.forename: Bo-Hye
authors.initials: BH
authors.lastname: Nam
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Yong-Taek
authors.initials: YT
authors.lastname: Jung
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute (NFRDI), Gijang, Busan 619-705, South Korea.
authors.forename: Dong-Gyun
authors.initials: DG
authors.lastname: Kim
authors.affiliation: Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, South Korea.
authors.forename: Kyung Sook
authors.initials: KS
authors.lastname: Bae
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D014561:Urochordata / Q000382:microbiology*; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6