title: Studies on optimization of growth parameters for L-asparaginase production by Streptomyces ginsengisoli.
abstract: A species of Streptomyces, Streptomyces ginsengisoli, a river isolate, was evaluated for production of an enzyme, L-asparaginase, with multiple functions mainly anticancer activity. The actinomycete was subjected to submerged fermentation by "shake flask" method. The quantity of L-asparaginase produced was estimated as 3.23 μ mol/mL/min. The effect of various culture conditions on L-asparaginase production was studied by adopting a method of variation in one factor at a time. Of the various conditions tested, glucose (followed by starch) and peptone served as good carbon and nitrogen sources, respectively, for maximal production of enzyme at pH 8. The temperature of 30 °C and an incubation period of 5 days with 0.05 g% asparagine concentration were found to be optimum for L-asparaginase production.
authors.affiliation: Department of Microbiology, Abasaheb Garware College, Pune 411004, India.
authors.forename: Neelima
authors.initials: N
authors.lastname: Deshpande
authors.affiliation: Department of Microbiology, Abasaheb Garware College, Pune 411004, India.
authors.forename: Prachi
authors.initials: P
authors.lastname: Choubey
authors.affiliation: Department of Microbiology, Abasaheb Garware College, Pune 411004, India.
authors.forename: Manasi
authors.initials: M
authors.lastname: Agashe
mesh_terms: D001215:Asparaginase / Q000096:biosynthesis*; D002244:Carbon / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen / Q000378:metabolism; D013302:Streptomyces / Q000254:growth & development* / Q000378:metabolism*; D013696:Temperature
chemical_list: D003470:Culture Media; D002244:Carbon; D001215:Asparaginase; D009584:Nitrogen