title: Degradation of specific nuclear proteins occurs in the cytoplasm in Saccharomyces cerevisiae.
abstract: The ubiquitin/proteasome system has been characterized extensively, although the site of nuclear substrate turnover has not been established definitively. We report here that two well-characterized nuclear proteins are stabilized in nuclear export mutants in Saccharomyces cerevisiae. The requirement for nuclear export defines a new regulatory step in intracellular proteolysis.
authors.affiliation: Department of Pharmacology, Robert Wood Johnson Medical School, Rutgers University, Piscataway, New Jersey 08854.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: Kiran
authors.initials: K
authors.lastname: Madura
mesh_terms: D021581:Active Transport, Cell Nucleus; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism*; D004256:DNA Polymerase I / Q000737:chemistry / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D055550:Protein Stability; D059748:Proteolysis; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C493797:Rad4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D004256:DNA Polymerase I; C083536:POL1 protein, S cerevisiae; D046988:Proteasome Endopeptidase Complex
keywords: degradation; export; proteasome; substrate; ubiquitin