title: Three new triterpene saponins from roots of Eryngium planum.
abstract: Saponin composition of the roots of Eryngium planum L. was investigated. Triterpene saponins found in E. planum and also present in Eryngium maritimum were different from those described previously in Eryngium campestre L. Three primary saponins were isolated and their tentative identifications, based on the electrospray MS/MS fragmentation patterns, were subsequently confirmed by 1D and 2D NMR analyses. Their structures were established as 3-O-β-D-glucopyranosyl-(1 → 2)-β-D-glucuronopyranosyl-21-O-acetyl-22-O-angeloyl-R1-barrigenol (1) and 3-O-β-D-glucopyranosyl-(1 → 2)-β-D-glucuronopyranosyl-22-O-angeloyl-A1-barrigenol (2) and 3-O-β-D-glucopyranosyl-(1 → 2)-β-D-glucuronopyranosyl-22-O-angeloyl-R1-barrigenol (3). Concentrations of the newly identified compounds in aerial parts and roots of both species were estimated using the liquid chromatography-mass spectrometry method.
authors.affiliation: a Department of Biochemistry , Institute of Soil Science and Plant Cultivation, State Research Institute , Puławy , Poland.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Kowalczyk
authors.forename: Milena
authors.initials: M
authors.lastname: Masullo
authors.forename: Barbara
authors.initials: B
authors.lastname: Thiem
authors.forename: Sonia
authors.initials: S
authors.lastname: Piacente
authors.forename: Anna
authors.initials: A
authors.lastname: Stochmal
authors.forename: Wiesław
authors.initials: W
authors.lastname: Oleszek
mesh_terms: D031049:Eryngium / Q000737:chemistry*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D018517:Plant Roots / Q000737:chemistry; D011044:Poland; D012503:Saponins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D014315:Triterpenes / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: C507467:3-O-beta-D-glucopyranosyl-(1-2)-(alpha-L-rhamnopyranosyl-(1-4))-beta-D-glucuronopyranosyl-22-O-angeloyl-R1-barrigenol; C000591769:3-O-beta-D-glucopyranosyl-(1-2)-beta-D-glucuronopyranosyl-21-O-acetyl-22-O-angeloyl-R1-barrigenol; C000591770:3-O-beta-D-glucopyranosyl-(1-2)-beta-D-glucuronopyranosyl-22-O-angeloyl-A1-barrigenol; C000591771:3-O-beta-D-glucopyranosyl-(1-2)-beta-D-glucuronopyranosyl-22-O-angeloyl-R1-barrigenol; D012503:Saponins; D014315:Triterpenes
keywords: Eryngium; barrigenol; liquid chromatography–mass spectrometry; nuclear magnetic resonance; triterpene saponins