title: Developments in x-ray crystallographic structure determination of biological macromolecules.
abstract: The three-dimensional structures of large biomolecules important in the function and mechanistic pathways of all living systems and viruses can be determined by x-ray diffraction from crystals of these molecules and their complexes. This area of crystallography is continually expanding and evolving, and the introduction of new methods that use the latest technology is allowing the elucidation of ever larger and more complex biological systems, which are now becoming tractable to structure solution. This review looks back at what has been achieved and forward at how current and future developments may allow technical challenges to be overcome.
authors.affiliation: Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
authors.forename: Elspeth F
authors.initials: EF
authors.lastname: Garman
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D018360:Crystallography, X-Ray / Q000295:instrumentation / Q000379:methods / Q000639:trends; D006454:Hemoglobins / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry; D009113:Muramidase / Q000737:chemistry; D011487:Protein Conformation
chemical_list: D001426:Bacterial Proteins; D006454:Hemoglobins; D008565:Membrane Proteins; D009113:Muramidase