title: Antibacterial, anti-inflammatory and probiotic potential of Enterococcus hirae isolated from the rumen of Bos primigenius.
abstract: In the present study bacterial strains were isolated from the rumen fluids of Bos primigenius and investigated their in vitro probiotic properties with potent antibacterial activity and anti-inflammatory effects. 9 g positive bacterial isolates were obtained and three isolates could able to tolerate gastric conditions, high bile salt concentrations and exhibited significant bactericidal effect against the enteric pathogens Vibrio cholera, Enterococcus faecalis, Enterobacter aerogens, Pseudomonas aeruginosa, Escherichia coli and Salmonella typhi. Moreover it showed above 70% cell surface hydrophobicity, significant low-invasion ability and potential adherence capacity in Caco-2 cells when compared with the control. The proinflammatory cytokines (TNF-α) was greatly reduced in rumen bacteria treatment and ARBS-1 modulate the immune response by activating the IL-4 secretion in parallel to TNF-α suppression. The 16s rRNA gene sequence of the active isolates were identified as Enterococcus hirae (ARBS-1), Pediococcus acidilactici (ARBS-4) and Bacillus licheniformis (ARBS-7). This study revealed the probiotic bactericidal properties of E. hirae obtained from the rumen of B. primigenius with potential antibacterial and anti-inflammatory effects. Future studies with the strains may yield some novel probiotic product for livestock's.
authors.affiliation: College of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
authors.forename: Selvaraj
authors.initials: S
authors.lastname: Arokiyaraj
authors.forename: Villianur Ibrahim
authors.initials: VI
authors.lastname: Hairul Islam
authors.forename: R
authors.initials: R
authors.lastname: Bharanidharan
authors.forename: Sebastian
authors.initials: S
authors.lastname: Raveendar
authors.forename: Jinwook
authors.initials: J
authors.lastname: Lee
authors.forename: Do Hyung
authors.initials: DH
authors.lastname: Kim
authors.forename: Young Kyoon
authors.initials: YK
authors.lastname: Oh
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Kim
authors.forename: Kyoung Hoon
authors.initials: KH
authors.lastname: Kim
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002417:Cattle / Q000382:microbiology*; D016983:Enterococcus / Q000187:drug effects*; D019936:Probiotics / Q000494:pharmacology*; D012417:Rumen / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents