title: Densified electrochemical sensors based on local redox cycling between vertically separated electrodes in substrate generation/chip collection and extended feedback modes.
abstract: A new local redox cycling-based electrochemical (LRC-EC) device integrated with many electrochemical sensors has been developed into a small chip device. The LRC-EC chip device was successfully applied for detection of alkaline phosphatase and horseradish peroxidase activity in substrate generation/chip collection (SG/CC) and extended feedback modes, respectively. The new imaging approach with extended feedback mode was particularly effective for sharpening of the image, because this mode uses feedback signals and minimizes the undesired influence of diffusion. The LRC-EC chip device is considered to be a useful tool for bioanalysis.
authors.affiliation: Graduate School of Environmental Studies and §WPI Advanced Institute for Materials Research, Tohoku University , Sendai, Japan 980-8579.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Ino
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kanno
authors.forename: Taku
authors.initials: T
authors.lastname: Nishijo
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Komaki
authors.forename: Yuta
authors.initials: Y
authors.lastname: Yamada
authors.forename: Shinya
authors.initials: S
authors.lastname: Yoshida
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Shiku
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Matsue
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D002478:Cells, Cultured; D004058:Diffusion; D004563:Electrochemistry / Q000295:instrumentation*; D004566:Electrodes; D058732:Embryoid Bodies / Q000201:enzymology; D004867:Equipment Design; D006735:Horseradish Peroxidase / Q000032:analysis; D051379:Mice; D008838:Microcomputers; D010084:Oxidation-Reduction
chemical_list: D006735:Horseradish Peroxidase; D000469:Alkaline Phosphatase