title: A highly selective turn-on fluorescent probe for Al(III) based on coumarin and its application in vivo.
abstract: In this study, a turn-on coumarin-based fluorescent probe, 7-hydroxy-6-[(2-hydroxy-naphthalen-1-ylmethylene)-amino]-4-methyl-chroman-2-one (CN), was developed for detecting Al(3+) in aqueous systems. The binding ratio of CN-Al(3+) complexes was determined from the Job plot and ESI-MS data to be 1 : 1. The binding constant (Ka) of Al(3+) binding to CN was calculated to be 9.55 × 10(4) M(-1) from a Benesi-Hildebrand plot and the detection limit was evaluated to be as low as 0.10 μM (LOD = 3σ/slope). CN could be used as an effective fluorescent probe for detecting Al(3+) in living HeLa cells. Moreover, CN could also be applied in the in vivo detection of Al(3+) in zebrafish.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, People's Republic of China. yingui@nju.edu.cn.
authors.forename: Hongde
authors.initials: H
authors.lastname: Xiao
authors.forename: Kun
authors.initials: K
authors.lastname: Chen
authors.forename: Nannan
authors.initials: N
authors.lastname: Jiang
authors.forename: Dandan
authors.initials: D
authors.lastname: Cui
authors.forename: Gui
authors.initials: G
authors.lastname: Yin
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Ruiyong
authors.initials: R
authors.lastname: Wang
mesh_terms: D000535:Aluminum / Q000032:analysis*; D000818:Animals; D003374:Coumarins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D013050:Spectrometry, Fluorescence; D021241:Spectrometry, Mass, Electrospray Ionization; D015027:Zebrafish
chemical_list: D003374:Coumarins; D005456:Fluorescent Dyes; C030123:coumarin; D000535:Aluminum