title: Protection from SARS coronavirus conferred by live measles vaccine expressing the spike glycoprotein.
abstract: The recent identification of a novel human coronavirus responsible of a SARS-like illness in the Middle-East a decade after the SARS pandemic, demonstrates that reemergence of a SARS-like coronavirus from an animal reservoir remains a credible threat. Because SARS is contracted by aerosolized contamination of the respiratory tract, a vaccine inducing mucosal long-term protection would be an asset to control new epidemics. To this aim, we generated live attenuated recombinant measles vaccine (MV) candidates expressing either the membrane-anchored SARS-CoV spike (S) protein or its secreted soluble ectodomain (Ssol). In mice susceptible to measles virus, recombinant MV expressing the anchored full-length S induced the highest titers of neutralizing antibodies and fully protected immunized animals from intranasal infectious challenge with SARS-CoV. As compared to immunization with adjuvanted recombinant Ssol protein, recombinant MV induced stronger and Th1-biased responses, a hallmark of live attenuated viruses and a highly desirable feature for an antiviral vaccine.
authors.affiliation: Institut Pasteur, Unité de Génétique Moléculaire des Virus à ARN, Département de Virologie, F-75015 Paris, France; CNRS, UMR 3569, F-75015 Paris, France; Univ. Paris Diderot, Sorbonne, Paris Cité, EA 302, F-75015 Paris, France. Electronic address: nicolas.escriou@pasteur.fr.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Escriou
authors.affiliation: Institut Pasteur, Unité de Génétique Moléculaire des Virus à ARN, Département de Virologie, F-75015 Paris, France; CNRS, UMR 3569, F-75015 Paris, France; Univ. Paris Diderot, Sorbonne, Paris Cité, EA 302, F-75015 Paris, France.
authors.forename: Benoît
authors.initials: B
authors.lastname: Callendret
authors.affiliation: Institut Pasteur, Unité de Génétique Moléculaire des Virus à ARN, Département de Virologie, F-75015 Paris, France; CNRS, UMR 3569, F-75015 Paris, France; Univ. Paris Diderot, Sorbonne, Paris Cité, EA 302, F-75015 Paris, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Lorin
authors.affiliation: Institut Pasteur, Unité de Génomique Virale et Vaccination, Département de Virologie, F-75015 Paris, France; CNRS, UMR 3569, F-75015 Paris, France.
authors.forename: Chantal
authors.initials: C
authors.lastname: Combredet
authors.affiliation: Institut Pasteur, Unité de Biologie des Infections Virales Emergentes, Département de Virologie, F-69007 Lyon, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Marianneau
authors.affiliation: Institut Pasteur, Unité de Génomique Virale et Vaccination, Département de Virologie, F-75015 Paris, France; CNRS, UMR 3569, F-75015 Paris, France.
authors.forename: Michèle
authors.initials: M
authors.lastname: Février
authors.affiliation: Institut Pasteur, Unité de Génomique Virale et Vaccination, Département de Virologie, F-75015 Paris, France; CNRS, UMR 3569, F-75015 Paris, France. Electronic address: frederic.tangy@pasteur.fr.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Tangy
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D005822:Genetic Vectors / Q000235:genetics / Q000276:immunology; D006801:Humans; D008297:Male; D008458:Measles Vaccine / Q000235:genetics / Q000276:immunology; D008459:Measles virus / Q000235:genetics* / Q000276:immunology; D051379:Mice; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D045169:Severe Acute Respiratory Syndrome / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D064370:Spike Glycoprotein, Coronavirus / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D008458:Measles Vaccine; D064370:Spike Glycoprotein, Coronavirus; D014765:Viral Vaccines; C578557:spike glycoprotein, SARS-CoV
keywords: Coronavirus; Measles vaccine; Severe acute respiratory syndrome; Spike glycoprotein