title: White spot syndrome virus VP12 interacts with adenine nucleotide translocase of Litopenaeus vannamei.
abstract: White spot syndrome virus VP12 contains cell attachment motif RGD which is considered to be critical for host cell binding. Until now, the function of this protein remains undefined. In this study, we explored the interaction of VP12 with host cells. A new shrimp protein (adenine nucleotide translocase of Litopenaeus vannamei, LvANT) is selected by far-western overlay assay. Tissue distribution of adenine nucleotide translocase mRNA showed that it was commonly spread in all the tissues detected. Cellular localization of LvANT in shrimp hemocytes showed that it was primarily located in the cytoplasm of hemocytes and colocalized with mitochondria. ELISA and far-western blot assay confirmed that VP12 interacted with LvANT. In vivo neutralization assay showed that anti-LvANT antibody can significantly reduce the mortality of shrimp challenged by WSSV at 48h post-treatment. Our results collectively showed that VP12 is involved in host cell binding via interaction with adenine nucleotide translocase.
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, PR China; Shanghai Ocean University, 999, Hucheng Ring Road, Pudong, Shanghai, PR China.
authors.forename: Fang-fang
authors.initials: FF
authors.lastname: Ma
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, PR China.
authors.forename: Zhi-guang
authors.initials: ZG
authors.lastname: Chou
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, PR China. Electronic address: liuqh@ysfri.ac.cn.
authors.forename: Qing-hui
authors.initials: QH
authors.lastname: Liu
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, PR China; Shanghai Ocean University, 999, Hucheng Ring Road, Pudong, Shanghai, PR China.
authors.forename: Guangkuo
authors.initials: G
authors.lastname: Guan
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, PR China. Electronic address: huangjie@ysfri.ac.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Huang
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D006790:Host-Parasite Interactions / Q000502:physiology*; D013058:Mass Spectrometry; D000226:Mitochondrial ADP, ATP Translocases / Q000378:metabolism*; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000821:virology*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism*; D046848:White spot syndrome virus 1 / Q000378:metabolism / Q000472:pathogenicity*
chemical_list: D011994:Recombinant Proteins; D014764:Viral Proteins; D000226:Mitochondrial ADP, ATP Translocases
keywords: Adenine nucleotide translocase; Litopenaeus vannamei; VP12; White spot syndrome virus