title: Plastid transformation in potato: Solanum tuberosum.
abstract: Although plastid transformation has attractive advantages and potential applications in plant biotechnology, for long time it has been highly efficient only in tobacco. The lack of efficient selection and regeneration protocols and, for some species, the inefficient recombination using heterologous flanking regions in transformation vectors prevented the extension of the technology to major crops. However, the availability of this technology for species other than tobacco could offer new possibilities in plant breeding, such as resistance management or improvement of nutritional value, with no or limited environmental concerns. Herein we describe an efficient plastid transformation protocol for potato (Solanum tuberosum subsp. tuberosum). By optimizing the tissue culture system and using transformation vectors carrying homologous potato flanking sequences, we obtained up to one transplastomic shoot per bombardment. Such efficiency is comparable to that usually achieved in tobacco. The method described in this chapter can be used to regenerate potato transplastomic plants expressing recombinant proteins in chloroplasts as well as in amyloplasts.
authors.affiliation: CNR-IBBR, National Research Council of Italy, Institute of Biosciences and BioResources, Naples, Italy.
authors.forename: Vladimir T
authors.initials: VT
authors.lastname: Valkov
authors.forename: Daniela
authors.initials: D
authors.lastname: Gargano
authors.forename: Nunzia
authors.initials: N
authors.lastname: Scotti
authors.forename: Teodoro
authors.initials: T
authors.lastname: Cardi
mesh_terms: D019470:Biolistics / Q000379:methods*; D002736:Chloroplasts / Q000235:genetics*; D004351:Drug Resistance; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors; D018515:Plant Leaves / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics; D011198:Solanum tuberosum / Q000235:genetics*; D000198:Spectinomycin / Q000494:pharmacology; D014170:Transformation, Genetic; D019076:Transgenes / Q000235:genetics
chemical_list: D000198:Spectinomycin