title: Inhibition of retinoblastoma mRNA degradation through Poly (A) involved in the neuroprotective effect of berberine against cerebral ischemia.
abstract: Berberine is one kind of isoquinoline alkaloid with anti-apoptotic effects on the neurons suffering ischemia. To address the explanation for these activities, the berberine-induced cell cycle arrest during neurons suffering ischemia/reperfusion had been studied in the present study. According to the in vitro neurons with oxygen-glucose deprivation and in vivo ICR mice with cerebral ischemia/reperfusion, it was found that berberine could protect the mRNA of retinoblastoma (Rb) from degradation through its function on the poly(A) tail. The prolonged half-life of retinoblastoma 1 (gene of Rb, RB1) mRNA level secures the protein level of retinoblastoma, which facilitates cell cycle arrest of neurons in the process of ischemia/reperfusion and subsequently avoids cells entering in the apoptotic process. The poly(A) tail of RB1 mRNA, as a newly identified target of berberine, could help people focus on the interaction between berberine and mRNA to further understand the biological activities and functions of berberine.
authors.affiliation: MOE Key Laboratory of Protein Sciences, Laboratory of Molecular Pharmacology and Pharmaceutical Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing, China.
authors.forename: Yu-Shuang
authors.initials: YS
authors.lastname: Chai
authors.affiliation: MOE Key Laboratory of Protein Sciences, Laboratory of Molecular Pharmacology and Pharmaceutical Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing, China.
authors.forename: Zhi-Yi
authors.initials: ZY
authors.lastname: Yuan
authors.affiliation: MOE Key Laboratory of Protein Sciences, Laboratory of Molecular Pharmacology and Pharmaceutical Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Lei
authors.affiliation: Department of Neuro-Oncology, MD Anderson Cancer Center, University of Texas, Houston, Texas, United States of America.
authors.forename: Yu-Gang
authors.initials: YG
authors.lastname: Wang
authors.affiliation: MOE Key Laboratory of Protein Sciences, Laboratory of Molecular Pharmacology and Pharmaceutical Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Mathematics, Tulane University, New Orleans, Louisiana, United States of America.
authors.forename: Feng
authors.initials: F
authors.lastname: Du
authors.affiliation: MOE Key Laboratory of Protein Sciences, Laboratory of Molecular Pharmacology and Pharmaceutical Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Lu
authors.affiliation: MOE Key Laboratory of Protein Sciences, Laboratory of Molecular Pharmacology and Pharmaceutical Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing, China.
authors.forename: Jing-Fei
authors.initials: JF
authors.lastname: Jiang
authors.affiliation: MOE Key Laboratory of Protein Sciences, Laboratory of Molecular Pharmacology and Pharmaceutical Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing, China.
authors.forename: Dong-Ming
authors.initials: DM
authors.lastname: Xing
authors.affiliation: MOE Key Laboratory of Protein Sciences, Laboratory of Molecular Pharmacology and Pharmaceutical Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing, China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Du
mesh_terms: D000818:Animals; D017209:Apoptosis; D001599:Berberine / Q000494:pharmacology*; D002545:Brain Ischemia / Q000188:drug therapy*; D059447:Cell Cycle Checkpoints; D002470:Cell Survival / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects / Q000473:pathology; D008297:Male; D008813:Mice, Inbred ICR; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016716:PC12 Cells; D010766:Phosphorylation; D011401:Promoter Regions, Genetic; D011499:Protein Processing, Post-Translational; D020871:RNA Stability / Q000187:drug effects*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D015427:Reperfusion Injury / Q000517:prevention & control; D016160:Retinoblastoma Protein / Q000235:genetics* / Q000378:metabolism
chemical_list: D018696:Neuroprotective Agents; D012333:RNA, Messenger; D016160:Retinoblastoma Protein; D001599:Berberine