title: Role of Burkholderia pseudomallei biofilm formation and lipopolysaccharide in relapse of melioidosis.
abstract: We examined whether quantitative biofilm formation and/or lipopolysaccharide type of Burkholderia pseudomallei was associated with relapsing melioidosis. We devised a 1:4 nested case-control study in which both cases and controls were drawn from a cohort of patients with primary melioidosis. Paired isolates from 80 patients with relapse and single isolates from 184 patients without relapse were tested. Relapse was associated with biofilm formation of the primary infecting isolate (conditional OR 2.03; 95% CI 1.27-3.25; p 0.003), but not with lipopolysaccharide type (p 0.74). This finding highlights the importance of biofilm formation in relapsing melioidosis.
authors.affiliation: Department of Tropical Hygiene, Mahidol University, Bangkok, Thailand; Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: D
authors.initials: D
authors.lastname: Limmathurotsakul
authors.forename: A
authors.initials: A
authors.lastname: Paeyao
authors.forename: S
authors.initials: S
authors.lastname: Wongratanacheewin
authors.forename: N
authors.initials: N
authors.lastname: Saiprom
authors.forename: N
authors.initials: N
authors.lastname: Takpho
authors.forename: J
authors.initials: J
authors.lastname: Thaipadungpanit
authors.forename: N
authors.initials: N
authors.lastname: Chantratita
authors.forename: V
authors.initials: V
authors.lastname: Wuthiekanun
authors.forename: N P J
authors.initials: NP
authors.lastname: Day
authors.forename: S J
authors.initials: SJ
authors.lastname: Peacock
mesh_terms: D000328:Adult; D018441:Biofilms / Q000254:growth & development*; D016957:Burkholderia pseudomallei / Q000502:physiology*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D008554:Melioidosis / Q000382:microbiology*; D008875:Middle Aged; D012008:Recurrence
chemical_list: D008070:Lipopolysaccharides
keywords: Biofilm; lipopolysaccharide; melioidosis; pseudomallei; relapse