title: HIV-TAT mediated protein transduction of heme oxygenase-1 protects HaCaT cells from ionizing radiation.
abstract: OBJECTIVE
  To elucidate the effects of human keratinocyte-derived HaCaT cells (HIV-TAT) protein transduction domains (PTD) coupled heme oxygenase-1 (HO-1) fusion protein (TAT-HO-1) on radiation-induced human keratinocyte-derived HaCaT cells.
  
  
  METHODS
  This study was conducted between May 2010 and February 2013 in the School of Radiation Medicine and Protection, Soochow University, Suzhou, China. This experimental study was designed to explore the protective role of TAT-HO-1 in skin cells. The human HO-1 gene was fused with a gene fragment encoding TAT PTD to produce in-frame TAT-HO-1. The distribution of TAT-HO-1 was measured by immunostaining and Western blot. The radioprotection for TAT-HO-1 was determined using clonogenic assay. Alterations in apoptosis were analyzed by flow cytometry.
  
  
  RESULTS
  The expressed and purified TAT-HO-1 recombinant protein could be incorporated into human HaCaT cells. We then evaluated the protective role of TAT-HO-1 against ionizing radiation. The TAT-HO-1 attenuated the generation of reactive oxygen species and decreased HaCaT cell radiosensitivity to irradiation. Moreover, HaCaT cells pretreated with TAT-HO-1 resulted in less apoptosis by radiation. In addition, TAT-HO-1 could penetrate rat skin.
  
  
  CONCLUSIONS
  These results suggest that TAT-HO-1 can protect HaCaT cells from ionizing radiation.
authors.affiliation: Department of Gastroenterology, the Affiliated Jiangyin Hospital of Southeast University, Jiangyin, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Liu
authors.affiliation: School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Xue
authors.affiliation: School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Gu
authors.affiliation: Institute of Plasma Physics, Chinese Academy of Sciences, Hefei, China.
authors.forename: Jinyong
authors.initials: J
authors.lastname: Wu
authors.affiliation: School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China.
authors.forename: Han
authors.initials: H
authors.lastname: Cao
authors.affiliation: School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.affiliation: School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China.
authors.forename: Jundong
authors.initials: J
authors.lastname: Zhou
authors.affiliation: School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Cao
authors.affiliation: School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China.
authors.forename: Shuyu
authors.initials: S
authors.lastname: Zhang
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D051547:Heme Oxygenase-1 / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D011839:Radiation, Ionizing; D014161:Transduction, Genetic; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000502:physiology*
chemical_list: D017931:DNA Primers; D054322:tat Gene Products, Human Immunodeficiency Virus; D051547:Heme Oxygenase-1