title: Single-locus-sequence-based typing of blaOXA-51-like genes for rapid assignment of Acinetobacter baumannii clinical isolates to international clonal lineages.
abstract: Single-locus blaOXA-51-like sequence-based typing (SBT) was evaluated for its ability to determine correctly sequence types (STs) in Acinetobacter baumannii clinical isolates, in comparison with the Pasteur's multilocus sequence typing (MLST) reference method and 3-locus sequence typing (3-LST). The comparative study was performed in 585 multidrug-resistant (MDR) A. baumannii clinical isolates recovered from 21 hospitals located throughout Greece, Italy, Lebanon, and Turkey. The isolates belonged to nine clonal complexes (CCs) that correspond to 12 distinct sequence types (STs) and to one singleton ST. These clonal lineages predominate worldwide among nosocomial MDR A. baumannii strains. The most common clone was CC2 (ST2 and ST45; n=278 isolates) followed by CC1 (ST1 and ST20; n=155), CC25 (n=65), ST78 (n=62), CC15 (ST15 and ST84; n=9), CC10 (n=4), CC3 (n=4), CC6 (n=3), CC54 (n=3), and CC83 (n=2). Using the blaOXA-51-like SBT method, all 585 isolates of the study were typed and assigned correctly to the nine CCs and the singleton ST78. The 3-LST method was not able to classify isolates belonging to CC6, CC10, CC54, and CC83, which are not yet characterized in its database. The low-cost and convenient blaOXA-51-like SBT method, compared with 3-LST and MLST, discriminated all epidemic and sporadic lineages of our collection and could be effectively applied to type rapidly A. baumannii strains.
authors.affiliation: Department of Microbiology, Medical School, University of Athens, Athens, Greece.
authors.forename: Spyros
authors.initials: S
authors.lastname: Pournaras
authors.forename: Vasiliki
authors.initials: V
authors.lastname: Gogou
authors.forename: Maria
authors.initials: M
authors.lastname: Giannouli
authors.forename: Evangelia
authors.initials: E
authors.lastname: Dimitroulia
authors.forename: Konstantina
authors.initials: K
authors.lastname: Dafopoulou
authors.forename: Athanasios
authors.initials: A
authors.lastname: Tsakris
authors.forename: Raffaele
authors.initials: R
authors.lastname: Zarrilli
mesh_terms: D000151:Acinetobacter Infections / Q000175:diagnosis* / Q000382:microbiology*; D040981:Acinetobacter baumannii / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D018432:Drug Resistance, Multiple / Q000235:genetics; D006761:Hospitals; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D017720:Molecular Epidemiology / Q000379:methods; D058885:Multilocus Sequence Typing / Q000379:methods*; D010802:Phylogeny
chemical_list: D001426:Bacterial Proteins