title: CD11b+ migratory dendritic cells mediate CD8 T cell cross-priming and cutaneous imprinting after topical immunization.
abstract: Topical antigen application is a focus of current vaccine research. This immunization route mimics natural antigen exposure across a barrier tissue and generates T cells imprinted for skin-selective homing. Soluble antigens introduced through this route require cross-presentation by DC to generate CD8 T cell responses. Here we have explored the relative contribution of various skin-derived DC subsets to cross-priming and skin-selective imprinting. In our model, DC acquire soluble Ag in vivo from immunized murine skin for cross-presentation to naïve CD8 T cells ex vivo. We find CD11b(+) migratory DC to be the relevant cross-priming DC in this model. Both Langerin(+) and Langerin(-) CD11b(+) migratory DC can cross-present antigen in our system, but only the Langerin+ subset can induce expression of the skin-selective addressin E-selectin ligand. Thus, the CD11b(+) Langerin(+) migratory DC population, comprised primarily of Langerhans cells, both cross-primes naïve CD8 T cells and imprints them with skin-homing capabilities.
authors.affiliation: Department of Dermatology, Brigham and Women's Hospital, Boston, Massachusetts, United States of America; Graduate Program in Biological and Biomedical Sciences, Harvard Medical School, Boston, Massachusetts, United States of America.
authors.forename: Suzanne T
authors.initials: ST
authors.lastname: Nizza
authors.affiliation: Department of Dermatology, Brigham and Women's Hospital, Boston, Massachusetts, United States of America; Department of Dermatology, Harvard Medical School, Boston, Massachusetts, United States of America.
authors.forename: James J
authors.initials: JJ
authors.lastname: Campbell
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D000954:Antigens, Surface / Q000378:metabolism; D039481:CD11b Antigen / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002633:Chemotaxis / Q000276:immunology; D003429:Cross Reactions / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism*; D015870:Gene Expression; D007114:Immunization; D037181:Lectins, C-Type / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology; D037241:Mannose-Binding Lectins / Q000378:metabolism; D051379:Mice; D017468:Receptors, Fibroblast Growth Factor / Q000235:genetics / Q000378:metabolism; D012795:Sialoglycoproteins / Q000235:genetics / Q000378:metabolism; D012867:Skin / Q000276:immunology* / Q000378:metabolism
chemical_list: D000941:Antigens; D000954:Antigens, Surface; D039481:CD11b Antigen; C498497:Cd207 protein, mouse; D037181:Lectins, C-Type; D037241:Mannose-Binding Lectins; D017468:Receptors, Fibroblast Growth Factor; D012795:Sialoglycoproteins; C086711:cysteine-rich fibroblast growth factor receptor