title: Two new anti-apoptotic proteins of white spot syndrome virus that bind to an effector caspase (PmCasp) of the giant tiger shrimp Penaeus (Penaeus) monodon.
abstract: White spot syndrome virus proteins WSSV134 and WSSV322 have been shown to bind with the p20 domain (residues 55-214) of Penaeus monodon caspase (PmCasp) protein through yeast two-hybrid screening. Binding was confirmed for the p20 domain and the full-length caspase by co-immunoprecipitation. WSSV134 is also known as the WSSV structural protein VP36A, but no function or conserved domains have been ascribed to WSSV322. Discovery of the caspase binding activity of these two proteins led to an investigation of their possible anti-apoptotic roles. Full-length PmCasp was confirmed to be an effector caspase by inducing apoptosis in transfected Sf-9 cells as assessed by DAPI staining. Using the same cell model, comparison of cells co-transfected with PmCasp and either WSSV134 or WSSV322 revealed that both of the binding proteins had anti-apoptotic activity. However, using the same Sf-9 protocol with anti-apoptosis protein-1 (AAP-1; also called WSSV449) previously shown to bind and inactivate a different effector caspase from P. monodon (Pm caspase) did not block apoptosis induced by PmCasp. The results revealed diversity in effector caspases and their viral protein inhibitors in P. monodon.
authors.affiliation: Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Center of Excellence for Shrimp Molecular Biology and Biotechnology, Mahidol University, Bangkok 10400, Thailand.
authors.forename: Tareerat
authors.initials: T
authors.lastname: Lertwimol
authors.affiliation: Center of Excellence for Shrimp Molecular Biology and Biotechnology, Mahidol University, Bangkok 10400, Thailand; Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Bangkok 10330, Thailand; National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani 12120, Thailand.
authors.forename: Pakkakul
authors.initials: P
authors.lastname: Sangsuriya
authors.affiliation: Center of Excellence for Shrimp Molecular Biology and Biotechnology, Mahidol University, Bangkok 10400, Thailand; National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani 12120, Thailand.
authors.forename: Kornsunee
authors.initials: K
authors.lastname: Phiwsaiya
authors.affiliation: Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Center of Excellence for Shrimp Molecular Biology and Biotechnology, Mahidol University, Bangkok 10400, Thailand; National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani 12120, Thailand. Electronic address: saengchan@biotec.or.th.
authors.forename: Saengchan
authors.initials: S
authors.lastname: Senapin
authors.affiliation: Center for Genomic and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Songkhla 90112, Thailand.
authors.forename: Amornrat
authors.initials: A
authors.lastname: Phongdara
authors.affiliation: Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
authors.forename: Chuenchit
authors.initials: C
authors.lastname: Boonchird
authors.affiliation: Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Center of Excellence for Shrimp Molecular Biology and Biotechnology, Mahidol University, Bangkok 10400, Thailand; National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani 12120, Thailand.
authors.forename: Timothy W
authors.initials: TW
authors.lastname: Flegel
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D020169:Caspases / Q000378:metabolism*; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D054884:Host-Pathogen Interactions; D007313:Insecta; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000201:enzymology* / Q000821:virology; D011485:Protein Binding; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D046848:White spot syndrome virus 1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D014764:Viral Proteins; D020169:Caspases
keywords: Apoptosis; Caspase; Shrimp; WSSV; Yeast two-hybrid