title: Shared epitopes of avian immunoglobulin light chains.
abstract: Like all jawed vertebrates, birds (Aves) also produce antibodies i.e. immunoglobulins (Igs) as a defence mechanism against pathogens. Their Igs are composed of two identical heavy (H) and light (L) chains which are of lambda isotype. The L chain consists of variable (VL), joining (JL) and constant (CL) region. Using enzyme immunoassays (EIA) and two monoclonal antibodies (mAbs) (3C10 and CH31) to chicken L chain, we analysed their cross-reactivity with sera from 33 avian species belonging to nine different orders. Among Galliformes tested, mAbs 3C10 and CH31 reacted with L chains of chicken, turkey, four genera of pheasants, tragopan and peafowl, but not with sera of grey partridge, quail and Japanese quail. Immunoglobulins of guinea-fowl reacted only with mAb 3C10. Both mAbs reacted also with the L chain of Eurasian griffon (order Falconiformes) and domestic sparrow (order Passeriformes). Sera from six other orders of Aves did not react with either of the two mAbs. EIA using mAbs 3C10 and CH31 enabled detection of antibodies to major avian pathogens in sera of chickens, turkeys, pheasants, peafowl, Eurasian griffon and guinea-fowl (only with mAb 3C10). The N-terminal amino acid sequence of pheasant L chain (19 residues) was identical to that of chicken. Sequences of genes encoding the L chain constant regions of pheasants, turkey and partridge were determined and deposited in the public database (GenBank accession numbers: FJ 649651, FJ 649652 and FJ 649653, respectively). Among them, amino acid sequence of pheasants is the most similar to that of chicken (97% similarity), whereas those of turkey and partridge have greater similarity to each other (89%) than to any other avian L chain sequence. The characteristic deletion of two amino acids which is present in the L chain constant region in Galliformes has been most likely introduced to their L chain after their divergence from Anseriformes.
authors.affiliation: Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, SI-1230 Domžale, Slovenia.
authors.forename: Mateja
authors.initials: M
authors.lastname: Benčina
authors.affiliation: Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, SI-1230 Domžale, Slovenia.
authors.forename: Ivanka
authors.initials: I
authors.lastname: Cizelj
authors.affiliation: Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, SI-1230 Domžale, Slovenia.
authors.forename: Rebeka Lucijana
authors.initials: RL
authors.lastname: Berčič
authors.affiliation: Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, SI-1230 Domžale, Slovenia.
authors.forename: Mojca
authors.initials: M
authors.lastname: Narat
authors.affiliation: Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, SI-1230 Domžale, Slovenia.
authors.forename: Dušan
authors.initials: D
authors.lastname: Benčina
authors.affiliation: Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, SI-1230 Domžale, Slovenia. Electronic address: peter.dovc@bf.uni-lj.
authors.forename: Peter
authors.initials: P
authors.lastname: Dovč
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D030161:Avian Proteins / Q000235:genetics* / Q000276:immunology*; D001717:Birds / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D002645:Chickens / Q000235:genetics / Q000276:immunology; D003429:Cross Reactions; D000939:Epitopes / Q000235:genetics; D007124:Immunoenzyme Techniques; D007127:Immunoglobulin Constant Regions / Q000235:genetics; D007133:Immunoglobulin Joining Region / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D000911:Antibodies, Monoclonal; D030161:Avian Proteins; D000939:Epitopes; D007127:Immunoglobulin Constant Regions; D007133:Immunoglobulin Joining Region; D007147:Immunoglobulin Light Chains
keywords: Aves; Cross-reactivity; L chains; Sequences; mAbs