title: Fine mapping and candidate gene analysis of the novel thermo-sensitive genic male sterility tms9-1 gene in rice.
abstract: Fine mapping of the novel thermo-sensitive genic male sterility locus tms9 - 1 in the traditional TGMS line HengnongS-1 revealed that the MALE STERILITY1 homolog OsMS1 is the candidate gene. Photoperiod-thermo-sensitive genic male sterility (P/TGMS) has been widely used in the two-line hybrid rice breeding system. HengnongS-1 is one of the oldest TGMS lines and is often used in indica two-line breeding programs in China. In this study, our genetic analysis showed that the TGMS gene in HengnongS-1 was controlled by a single recessive gene that was non-allelic with the other TGMS loci identified, including C815S, Zhu1S and Y58S. Using SSR markers and bulked segregant analysis, we located the TGMS locus on chromosome 9 and named the gene tms9-1. Fine mapping further narrowed the tms9-1 loci to a 162 kb interval between two dCAPS markers. Sequence analysis revealed that a T to C substitution results in an amino acid change in the tms9-1 candidate gene (Os09g27620) in HengnongS-1 as compared to Minghui63. Sequencing of other rice accessions, including six P/TGMS lines, seven indica varieties and nine japonica varieties, showed that this SNP was exclusive to HengnongS-1. With multiple sequence alignment and expression pattern analyses, the rice MALE STERILITY1 homolog OsMS1 gene was identified as the candidate gene for tms9-1. Therefore, our study identified a novel TGMS locus and will facilitate the functional identification of the tms9-1 gene. Moreover, the markers linked to the tms9-1 gene will provide useful tools for the development of new TGMS lines by marker-assisted selection in two-line hybrid rice breeding programs.
authors.affiliation: College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, Zhejiang, China.
authors.forename: Yongbin
authors.initials: Y
authors.lastname: Qi
authors.forename: Qinglong
authors.initials: Q
authors.lastname: Liu
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Bizeng
authors.initials: B
authors.lastname: Mao
authors.forename: Dawei
authors.initials: D
authors.lastname: Yan
authors.forename: Qingsheng
authors.initials: Q
authors.lastname: Jin
authors.forename: Zuhua
authors.initials: Z
authors.lastname: He
mesh_terms: D002874:Chromosome Mapping; D017343:Genes, Plant; D056726:Genetic Association Studies; D006824:Hybridization, Genetic; D012275:Oryza / Q000033:anatomy & histology / Q000235:genetics* / Q000502:physiology; D051479:Plant Infertility / Q000235:genetics*; D013696:Temperature