title: Single-molecule motions enable direct visualization of biomolecular interactions in solution.
abstract: Biomolecular interactions are generally accompanied by modifications in size and charge of biomolecules at the nanometer scale. Here we describe a single-molecule method to sense these changes in real time based on statistical learning of diffusive and electric field-induced motion parameters of a trapped molecule in solution. We demonstrate the approach by resolving a monomer-trimer mixture along a protein dissociation pathway and visualizing the binding-unbinding kinetics of a single DNA molecule.
authors.affiliation: 1] Department of Chemistry, Stanford University, Stanford, California, USA. [2] Department of Electrical Engineering, Stanford University, Stanford, California, USA.
authors.forename: Quan
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Stanford University, Stanford, California, USA.
authors.forename: W E
authors.initials: WE
authors.lastname: Moerner
mesh_terms: D000465:Algorithms; D055592:Biophysical Phenomena; D004247:DNA / Q000737:chemistry*; D004058:Diffusion; D004563:Electrochemistry; D007700:Kinetics; D008390:Markov Chains; D015233:Models, Statistical; D009038:Motion; D016011:Normal Distribution; D009693:Nucleic Acid Hybridization; D017785:Photons; D012984:Software; D012996:Solutions; D012997:Solvents / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D053545:Spirulina / Q000737:chemistry; D013997:Time Factors
chemical_list: D012996:Solutions; D012997:Solvents; D004247:DNA