title: Retinal overexpression of Ten-m3 alters ipsilateral retinogeniculate projections in the wallaby (Macropus eugenii).
abstract: The dorsal lateral geniculate nucleus (dLGN) contains a retinotopic map where input from the two eyes map in register to provide a substrate for binocular vision. Ten-m3, a transmembrane protein, mediates homophilic interactions and has been implicated in the patterning of ipsilateral visual projections. Ease of access to early developmental stages in a marsupial wallaby has been used to manipulate levels of Ten-m3 during the development of retinogeniculate projections. In situ hybridisation showed a high dorsomedial to low ventrolateral gradient of Ten-m3 in the developing dLGN, matching retinotopically with the previously reported high ventral to low dorsal retinal gradient. Overexpression of Ten-m3 in ventronasal but not dorsonasal retina resulted in an extension of ipsilateral projections beyond the normal binocular zone. These results demonstrate that Ten-m3 influences ipsilateral projections and support a role for it in binocular mapping.
authors.affiliation: Biomedical Sciences and Biochemistry, Research School of Biology, Australian National University, Canberra 0200, ACT, Australia. Electronic address: owen.carr@health.gov.au.
authors.forename: Owen P
authors.initials: OP
authors.lastname: Carr
authors.affiliation: Discipline of Physiology, School of Medical Sciences and Bosch Institute, University of Sydney, Sydney 2006, NSW, Australia. Electronic address: kelly.glendining@anatomy.otago.ac.nz.
authors.forename: Kelly A
authors.initials: KA
authors.lastname: Glendining
authors.affiliation: Discipline of Physiology, School of Medical Sciences and Bosch Institute, University of Sydney, Sydney 2006, NSW, Australia. Electronic address: cathy@physiol.usyd.edu.au.
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Leamey
authors.affiliation: Biomedical Sciences and Biochemistry, Research School of Biology, Australian National University, Canberra 0200, ACT, Australia. Electronic address: lauren.marotte@anu.edu.au.
authors.forename: Lauren R
authors.initials: LR
authors.lastname: Marotte
mesh_terms: D000818:Animals; D005829:Geniculate Bodies / Q000502:physiology*; D007614:Macropodidae / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D012160:Retina / Q000502:physiology*
chemical_list: D008565:Membrane Proteins
keywords: Development; Mapping; Odz; Teneurin; Visual system