title: Paraoxonase 1 lactonase activity and distribution in the HDL subclasses in the cord blood.
abstract: OBJECTIVE
  Paraoxonase 1 (PON1) is a lactonase with important antioxidant and immunoprotective properties. We hypothesized that PON1 lactonase activity, PON1, and high-density lipoprotein (HDL) subclasses distribution are different in neonates than in adults.
  
  
  METHODS
  We studied 83 healthy term neonates (34 males and 49 females) who were born by spontaneous, uncomplicated vaginal delivery. The study also included 17 paired maternal blood samples as well as 20 non-pregnant women collected for comparison. Total and free PON1 lactonase and arylesterase activity, HDL subclasses, PON1, and apolipoprotein distribution in the subclasses were assayed.
  
  
  RESULTS
  PON1 arylesterase activity in the cord blood represented 37% ± 4 of the maternal activity, whereas the PON1 lactonase activity amounted to only 23% ± 5 of the maternal activity. The free arylesterase and lactonase activities were higher in the cord blood by 16 and 36%, respectively. There is a 65% lower HDL2b PON1 in the cord blood than in the maternal serum. When the Lipoprint HDL subclasses were assayed, the neonates showed a larger content (52% higher) of very large HDL as well as a characteristic peak in the middle-sized HDL5 which is unremarkable in the mothers.
  
  
  CONCLUSIONS
  The novel findings of this study are that the neonates have lower PON1 lactonase activity, higher free PON1, different distributions of PON1 in the HDL subclasses as compared with their mother and adults as well as a distinctive HDL subclass lipid profile. Our data also suggest that the neonate HDL is enriched with an intermediate-sized (and/or less charged HDL) that is also rich in active PON1.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Gugliucci
authors.forename: Masahide
authors.initials: M
authors.lastname: Numaguchi
authors.forename: Russell
authors.initials: R
authors.lastname: Caccavello
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kimura
mesh_terms: D000328:Adult; D043303:Aryldialkylphosphatase / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D005312:Fetal Blood / Q000201:enzymology*; D006801:Humans; D007231:Infant, Newborn; D008075:Lipoproteins, HDL / Q000097:blood* / Q000737:chemistry*; D008297:Male; D011247:Pregnancy
chemical_list: D008075:Lipoproteins, HDL; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human
keywords: Arylesterase; Cardiovascular risk; High-density lipoprotein subclasses; Lactonase; Newborn HDL composition; PON1