title: Combined effects of oxytetracycline and Pb on earthworm Eisenia fetida.
abstract: Combined effects of oxytetracycline (OTC) and Pb on lysosomal membrane stability and coelomocyte apoptosis of earthworm were studied in the paper. Compared with control, the lysosomal membrane stability decreased and coelomocyte apoptosis increased in the treatments of single OTC and Pb contamination. As for compound pollution, combined effect of (5 mg/kg OTC+50 mg/kg Pb) treatment on earthworm lysosomal was synergistic (except 28 d). However, it was antagonistic at higher concentration of (10 mg/kg OTC+50 mg/kg Pb) and (20 mg/kg OTC+50 mg/kg Pb) treatment. In addition, coelomocyte apoptosis of earthworm decreased significantly compared with single OTC, indicating an antagonistic reaction. And joint toxicity of OTC and Pb decreased significantly with the increasing OTC concentration.
authors.affiliation: School of Environmental and Chemical Engineering, Tianjin Polytechnic University, No. 399 Binshui Western Road, Xiqing District, Tianjin 300387, China. Electronic address: kyforever2013@163.com.
authors.forename: Minling
authors.initials: M
authors.lastname: Gao
authors.affiliation: School of Environmental and Chemical Engineering, Tianjin Polytechnic University, No. 399 Binshui Western Road, Xiqing District, Tianjin 300387, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: School of Environmental and Chemical Engineering, Tianjin Polytechnic University, No. 399 Binshui Western Road, Xiqing District, Tianjin 300387, China.
authors.forename: Wenhua
authors.initials: W
authors.lastname: Song
authors.affiliation: School of Environmental and Chemical Engineering, Tianjin Polytechnic University, No. 399 Binshui Western Road, Xiqing District, Tianjin 300387, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Ma
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects; D004336:Drug Antagonism; D007854:Lead / Q000633:toxicity*; D007962:Leukocytes / Q000187:drug effects; D008247:Lysosomes / Q000187:drug effects; D009835:Oligochaeta / Q000187:drug effects*; D010118:Oxytetracycline / Q000633:toxicity*; D012989:Soil Pollutants / Q000633:toxicity*
chemical_list: D000900:Anti-Bacterial Agents; D012989:Soil Pollutants; D007854:Lead; D010118:Oxytetracycline
keywords: Coelomocyte apoptosis; Combined effects; Lysosomal membrane stability; Oxytetracycline; Pb