title: A potent antimicrobial peptide derived from the protein LsGRP1 of Lilium.
abstract: LsGRP1 is a defense-related gene differentially expressed in lily leaves in response to pathogen attack. The difficulty in the expression of LsGRP1 in Escherichia coli suggested the presence of antimicrobial activity in LsGRP1. To evaluate the antimicrobial trait of LsGRP1, three LsGRP1-derived peptides were chemically synthesized; namely LsGRP1(N) (N-terminal region without the signal peptide), LsGRP1(G) (glycine-rich region), and LsGRP1(C) (C-terminal cysteine-rich region). LsGRP1(C) was proposed to be a potential antimicrobial agent according to its broad-spectrum and effective antimicrobial activity. LsGRP1(C) displayed inhibition effects on bacterial and fungal growth, possibly by altering the integrity of the cell membrane, as indicated by scanning electron microscopy and SYTOX Green staining assays. Additionally, LsGRP1(C) induced programmed cell death-like phenomenon in the tested fungal species as indicated by 2',7'-dichlorodihydrofluorescein diacetate and 4',6'-diamidino-2-phenylindole assays. Further immunofluorescence staining showed that LsGRP1(C) was located at the fungal cell surface. According to these observations, we concluded that LsGRP1(C) originated from the plant defense-related protein LsGRP1 would play a role as an antimicrobial peptide and have a potential for practical use.
authors.forename: Chia-Hua
authors.initials: CH
authors.lastname: Lin
authors.forename: Min-Wei
authors.initials: MW
authors.lastname: Chang
authors.forename: Chao-Ying
authors.initials: CY
authors.lastname: Chen
mesh_terms: D000890:Anti-Infective Agents / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000138:chemical synthesis / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D001419:Bacteria / Q000166:cytology / Q000187:drug effects*; D002462:Cell Membrane / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects; D019295:Computational Biology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005658:Fungi / Q000166:cytology / Q000187:drug effects*; D025301:Hyphae; D027762:Lilium / Q000737:chemistry* / Q000235:genetics / Q000382:microbiology; D008856:Microscopy, Fluorescence; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000737:chemistry / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000138:chemical synthesis / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D010940:Plant Proteins