title: PTH-C1: a rat continuous cell line expressing the parathyroid phenotype.
abstract: The lack of a continuous cell line of epithelial parathyroid cells able to produce parathyroid hormone (PTH) has hampered the studies on in vitro evaluation of the mechanisms involved in the control of parathyroid cell function and proliferation. The PT-r cell line was first established from rat parathyroid tissue in 1987, but these cells were known to express the parathyroid hormone-related peptide (Pthrp) gene, but not the Pth gene. In an attempt to subclone the PT-r cell line, a rat parathyroid cell strain was isolated and named PTH-C1. During 3 years, in culture, PTH-C1 cells maintained an epithelioid morphology, displaying a diploid chromosome number, a doubling time around 15 h during the exponential phase of growth, and parathyroid functional features. PTH-C1 cell line produces PTH and expresses the calcium sensing receptor (Casr) gene and other genes known to be involved in parathyroid function. Most importantly, the PTH-C1 cells also exhibit an in vitro secretory response to calcium. Altogether these findings indicate the uniqueness of the PTH-C1 cell line as an in vitro model for cellular and molecular studies on parathyroid physiopathology.
authors.affiliation: Department of Surgery and Translational Medicine, University of Florence, Largo Brambilla 3, 50134, Florence, Italy.
authors.forename: Sergio
authors.initials: S
authors.lastname: Fabbri
authors.forename: Simone
authors.initials: S
authors.lastname: Ciuffi
authors.forename: Valeria
authors.initials: V
authors.lastname: Nardone
authors.forename: Ana Rita
authors.initials: AR
authors.lastname: Gomes
authors.forename: Carmelo
authors.initials: C
authors.lastname: Mavilia
authors.forename: Roberto
authors.initials: R
authors.lastname: Zonefrati
authors.forename: Gianna
authors.initials: G
authors.lastname: Galli
authors.forename: Ettore
authors.initials: E
authors.lastname: Luzi
authors.forename: Annalisa
authors.initials: A
authors.lastname: Tanini
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Brandi
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D010280:Parathyroid Glands / Q000166:cytology* / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D051381:Rats; D044169:Receptors, Calcium-Sensing / Q000235:genetics / Q000378:metabolism
chemical_list: D010281:Parathyroid Hormone; D044169:Receptors, Calcium-Sensing; C473605:extracellular calcium cation-sensing receptor, rat; D002118:Calcium