title: Novel influenza virus vectors expressing Brucella L7/L12 or Omp16 proteins in cattle induced a strong T-cell immune response, as well as high protectiveness against B. abortus infection.
abstract: This paper presents the results of a study of the immunogenicity and protectiveness of new candidate vector vaccine against Brucella abortus - a bivalent vaccine formulation consisting of a mixture of recombinant influenza A subtype H5N1 or H1N1 (viral constructs vaccine formulation) viruses expressing Brucella ribosomal protein L7/L12 and Omp16, in cattle. To increase the effectiveness of the candidate vaccine, adjuvants such as Montanide Gel01 or chitosan were included in its composition. Immunization of cattle (heifers aged 1-1.5 years, 5 animals per group) with the viral constructs vaccine formulation only, or its combination with adjuvants Montanide Gel01 or chitosan, was conducted via the conjunctival method using cross prime (influenza virus subtype H5N1) and booster (influenza virus subtype H1N1) vaccination schedules at an interval of 28 days. Vaccine candidates were evaluated in comparison with the positive (B. abortus S19) and negative (PBS) controls. The viral constructs vaccine formulations, particularly in combination with Montanide Gel01 adjuvant promoted formation of IgG antibodies (with a predominance of antibodies of isotype IgG2a) against Brucella L7/L12 and Omp16 proteins in ELISA. Moreover, these vaccines in cattle induced a strong antigen-specific T-cell immune response, as indicated by a high number of CD4(+) and CD8(+) cells, as well as the concentration of IFN-γ, and most importantly provided a high level of protectiveness comparable to the commercial B. abortus S19 vaccine and superior to the B. abortus S19 vaccine in combination with Montanide Gel01 adjuvant. Based on these findings, we recommended the bivalent vaccine formulation containing the adjuvant Montanide Gel01 for practical use in cattle.
authors.affiliation: The Research Institute for Biological Safety Problems, 080409, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeisky, Kazakhstan. Electronic address: tabynov_81@mail.ru.
authors.forename: Kaissar
authors.initials: K
authors.lastname: Tabynov
authors.affiliation: The Research Institute for Biological Safety Problems, 080409, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeisky, Kazakhstan.
authors.forename: Zhailaubay
authors.initials: Z
authors.lastname: Kydyrbayev
authors.affiliation: The Research Institute for Biological Safety Problems, 080409, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeisky, Kazakhstan.
authors.forename: Sholpan
authors.initials: S
authors.lastname: Ryskeldinova
authors.affiliation: The Research Institute for Biological Safety Problems, 080409, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeisky, Kazakhstan.
authors.forename: Bolat
authors.initials: B
authors.lastname: Yespembetov
authors.affiliation: The Research Institute for Biological Safety Problems, 080409, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeisky, Kazakhstan.
authors.forename: Nadezhda
authors.initials: N
authors.lastname: Zinina
authors.affiliation: The Research Institute for Biological Safety Problems, 080409, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeisky, Kazakhstan.
authors.forename: Nurika
authors.initials: N
authors.lastname: Assanzhanova
authors.affiliation: The Research Institute for Biological Safety Problems, 080409, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeisky, Kazakhstan.
authors.forename: Yerken
authors.initials: Y
authors.lastname: Kozhamkulov
authors.affiliation: The Research Institute for Biological Safety Problems, 080409, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeisky, Kazakhstan.
authors.forename: Dulat
authors.initials: D
authors.lastname: Inkarbekov
authors.affiliation: The Research Institute for Biological Safety Problems, 080409, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeisky, Kazakhstan.
authors.forename: Tatyana
authors.initials: T
authors.lastname: Gotskina
authors.affiliation: The Research Institute for Biological Safety Problems, 080409, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeisky, Kazakhstan.
authors.forename: Abylai
authors.initials: A
authors.lastname: Sansyzbay
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D002004:Brucella Vaccine / Q000276:immunology*; D002003:Brucella abortus; D002007:Brucellosis, Bovine / Q000517:prevention & control*; D002417:Cattle; D045142:Cross-Priming; D005260:Female; D007111:Immunity, Cellular; D007117:Immunization, Secondary; D007074:Immunoglobulin G / Q000097:blood; D053118:Influenza A Virus, H1N1 Subtype; D053124:Influenza A Virus, H5N1 Subtype; D007371:Interferon-gamma / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D002004:Brucella Vaccine; D007074:Immunoglobulin G; D007371:Interferon-gamma
keywords: Brucella abortus; Cattle; Influenza virus; Protectiveness; T-cell immune response; Vector vaccine