title: Genomics-guided discovery of endophenazines from Kitasatospora sp. HKI 714.
abstract: In this study we report on the genomics-guided exploration of the metabolic potential of the newly discovered strain Kitasatospora sp. HKI 714. The bioinformatics analysis of the whole genome sequence revealed the presence of a biosynthetic gene cluster presumably responsible for the biosynthesis of formerly unknown endophenazine derivatives. A 200 L cultivation combined with bioactivity-guided isolation techniques revealed four new natural products belonging to the endophenazines and the 5,10-dihydrophenazines. Detailed descriptions of their biological effects, mainly focused on antimicrobial properties against several mycobacteria, are given.
authors.affiliation: Leibniz Institute for Natural Product Research and Infection Biology , HKI , Beutenbergstraße 11a, 07745 Jena, Germany , and Friedrich Schiller University , Jena, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Heine
authors.forename: Karin
authors.initials: K
authors.lastname: Martin
authors.forename: Christian
authors.initials: C
authors.lastname: Hertweck
mesh_terms: D039903:Actinobacteria / Q000737:chemistry*; D001412:Bacillus subtilis / Q000187:drug effects; D001688:Biological Products / Q000378:metabolism; D002176:Candida albicans / Q000187:drug effects; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D013293:Enterococcus faecalis / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D023281:Genomics; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D009162:Mycobacterium avium / Q000187:drug effects; D019906:Nuclear Magnetic Resonance, Biomolecular; D010407:Penicillium / Q000187:drug effects; D010619:Phenazines / Q000737:chemistry / Q000302:isolation & purification*; D010802:Phylogeny; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012988:Soil Microbiology; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D001688:Biological Products; D004269:DNA, Bacterial; D010619:Phenazines