title: Proteomic analysis of enzyme production by Bacillus licheniformis using different feather wastes as the sole fermentation media.
abstract: This study evaluates the use of different types of feathers as fermentation media for enzyme production. Bacillus licheniformis was grown on the feathers, which lead to total biodegradation due to bacterial enzymatic hydrolytic excretion. B. licheniformis excretes protease and lipase activity, with feather concentration being the main parameter controlling their generation. Using a proteomic approach, the proteins excreted during fermentation were identified, and the influence of the chemical composition of the feathers on protein secretion was tested. The identified proteins are hydrolytic enzymes such as keratinase, gamma-glutamyltranspeptidase, chitosanases, and glicosidases. The diversity of proteins is related to the chemical complexity of the feathers. Understanding the composition of a hydrolytic system, when B. licheniformis is cultured on different feathers, may assist in utilizing such a system for producing different hydrolytic enzymes. The data indicate that proteomics can be a valuable tool for describing the physiological state of B. licheniformis cell populations growing on different wastes.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Sevilla, C/ Profesor García González 2, 41012 Sevilla, Spain. Electronic address: parrado@us.es.
authors.forename: J
authors.initials: J
authors.lastname: Parrado
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Sevilla, C/ Profesor García González 2, 41012 Sevilla, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Rodriguez-Morgado
authors.affiliation: Departamento de Cristalografía, Mineralogía y Química Agrícola, Universidad de Sevilla, Cta. Utrera Km 1, 41089 Sevilla, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Tejada
authors.affiliation: Department of Soil and Water Conservation and Organic Waste Management, CEBAS-CSIC, P.O. Box 164, 30100 Espinardo, Murcia, Spain.
authors.forename: T
authors.initials: T
authors.lastname: Hernandez
authors.affiliation: Department of Soil and Water Conservation and Organic Waste Management, CEBAS-CSIC, P.O. Box 164, 30100 Espinardo, Murcia, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Garcia
mesh_terms: D000818:Animals; D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development; D001426:Bacterial Proteins / Q000096:biosynthesis; D003470:Culture Media; D005241:Feathers / Q000737:chemistry; D005285:Fermentation; D006026:Glycoside Hydrolases / Q000096:biosynthesis; D007218:Industrial Microbiology; D007220:Industrial Waste / Q000032:analysis; D007633:Keratins / Q000378:metabolism; D010447:Peptide Hydrolases / Q000096:biosynthesis; D059748:Proteolysis; D040901:Proteomics; D005723:gamma-Glutamyltransferase / Q000096:biosynthesis
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D007220:Industrial Waste; D007633:Keratins; D005723:gamma-Glutamyltransferase; D006026:Glycoside Hydrolases; C019868:chitosanase; D010447:Peptide Hydrolases; C035855:keratinase
keywords: Bacillus licheniformis; Feathers; Lipase; Protease; Proteomic study