title: Genotypes and prevalence of HPV single and multiple concurrent infections in women with HSIL.
abstract: The contribution of human papillomavirus (HPV) types to the carcinogenesis of cervical cancer has been established for a long time. However, the role of phylogenetically related and rare variants remains uncertain, as well as the influence of concurrent multiple HPV genotypes infection. We aimed at studying the prevalence of several HPV genotypes infecting women with single versus concurrent multiple HPV genotypes infection with a HSIL diagnosis in a cervical cytology. We conducted a cross-sectional study using Thin-Prep(®) liquid-based cervical cytology specimens with the diagnosis of high-grade squamous intraepithelial lesion (HSIL), in which HPV genotype was sequentially tested. Genotypes were determined with a PapilloCheck(®) system, a DNA-Chip for the type-specific identification of 18 high-risk and six low-risk types of HPV. Of the total study population, 176 cases had a diagnosis of HSIL and positive HPV genotyping result, being HPV16 the most prevalent genotype (48.86%; 95%CI: 41.58-56.19) followed by HPV31 (14.20%; 95%CI: 9.75-20.18). Concurrent multiple HPV genotypes were detected in 36.93% (95%CI: 30.15-44.27) of the patients. The prevalence of the 10 most common HPV genotypes detected varied significantly according to the presence of single vs. concurrent multiple HPV genotypes (P = 0.022). Moreover, women with concurrent multiple HPV genotypes were on average 3.53 (95%CI: 0.43-6.64) years younger than women with single genotype infection. Our results suggest that women with multiple genotype HPV infection differ in terms of age and distribution of the most prevalent HPV genotypes. Additionally, we provide further evidence of the predominance of HPV16 in HSIL lesions of the uterine cervix.
authors.affiliation: IPATIMUP-Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal; Department of Pathology and Oncology, Faculty of Medicine, University of Porto, Portugal; Department of Pathology, Centro Hospitalar de São João, Porto, Portugal.
authors.forename: Francisco
authors.initials: F
authors.lastname: Beca
authors.forename: Jorge
authors.initials: J
authors.lastname: Pinheiro
authors.forename: Elisabete
authors.initials: E
authors.lastname: Rios
authors.forename: Patricia
authors.initials: P
authors.lastname: Pontes
authors.forename: Isabel
authors.initials: I
authors.lastname: Amendoeira
mesh_terms: D000328:Adult; D060085:Coinfection / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D003430:Cross-Sectional Studies; D005260:Female; D005838:Genotype; D061809:Human Papillomavirus DNA Tests; D052162:Human papillomavirus 16 / Q000235:genetics*; D006801:Humans; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D015995:Prevalence; D065310:Squamous Intraepithelial Lesions of the Cervix / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*
keywords: Gynaecological cytology; HPV; genotyping