title: Comparative pyrosequencing analysis of bacterial community change in biofilm formed on seawater reverse osmosis membrane.
abstract: The change in bacterial community structure induced by bacterial competition and succession was investigated during seawater reverse osmosis (SWRO) in order to elucidate a possible link between the bacterial consortium on SWRO membranes and biofouling. To date, there has been no definitive characterization of the microbial diversity in SWRO in terms of distinguishing time-dependent changes in the richness or abundance of bacterial species. For bacterial succession within biofilms on the membrane surface, SWRO using a cross-flow filtration membrane test unit was operated for 5 and 100h, respectively. As results of the pyrosequencing analysis, bacterial communities differed considerably among seawater and the 5 and 100 h samples. From a total of 33,876 pyrosequences (using a 95% sequence similarity), there were less than 1% of shared species, confirming the influence of the operational time factor and lack of similarity of these communities. During SWRO operation, the abundance of Pseudomonas stutzeri BBSPN3 (GU594474) belonging to gamma-Proteobacteria suggest that biofouling of SWRO membrane might be driven by the dominant influence of a specific species. In addition, among the bacterial competition of five bacterial species (Pseudomonas aeruginosa, Bacillus sp., Rhodobacter sp., Flavobacterium sp., and Mycobacterium sp.) competing for bacterial colonization on the SWRO membrane surfaces, it was exhibited that Bacillus sp. was the most dominant. The dominant influences ofPseudomonas sp. and Bacillus sp. on biofouling during actual SWRO is decisive depending on higher removal efficiency of the seawater pretreatment.
authors.affiliation: School of Environmental Science and Engineering, Gwangju Institute of Science and Technology, 261 Cheomdan-gwagiro, Buk-gu, Gwangju 500-712, Republic of Korea.
authors.forename: In S
authors.initials: IS
authors.lastname: Kim
authors.affiliation: Monitoring and Analysis Division, Wonju Regional Environmental Office, 171 Dangu-ro, Wonju-Si, Gangwon-do, 220-947, Republic of Korea.
authors.forename: Jinwook
authors.initials: J
authors.lastname: Lee
authors.affiliation: School of Environmental Science and Engineering, Gwangju Institute of Science and Technology, 261 Cheomdan-gwagiro, Buk-gu, Gwangju 500-712, Republic of Korea.
authors.forename: Sung-Jo
authors.initials: SJ
authors.lastname: Kima
authors.affiliation: Department of Soil, Water and Environmental Science, College of Agriculture and Life Sciences, University ofArizona, Tucson, Arizona, USA.
authors.forename: Hye-Weon
authors.initials: HW
authors.lastname: Yu
authors.affiliation: Department of Civil and Environmental Engineering, Sungkyunkwan University, Jangan-gu, Suwon, Gyeonggi-do 440-746, Republic of Korea.
authors.forename: Am
authors.initials: A
authors.lastname: Jang
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D018441:Biofilms / Q000145:classification* / Q000254:growth & development*; D008567:Membranes, Artificial; D059013:Microbial Consortia / Q000235:genetics*; D008969:Molecular Sequence Data; D009995:Osmosis; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014462:Ultrafiltration / Q000295:instrumentation*
chemical_list: D008567:Membranes, Artificial