title: Ex vivo converted double negative T cells suppress activated B cells.
abstract: Although the ability of endogenous CD4(-)CD8(-) double negative (DN) T cells to suppress B cells has been documented, the extent to which ex vivo converted DN T cells suppress B cells activity is still being explored. The aim of this study was to determine whether and what extent ex vivo converted CD4(-)CD8(-) DN T cells suppress B cell activation and antibody production. We found that ex vivo converted DN T cells suppressed proliferation of activated B cells in a perforin and cell-cell contact dependent manner. In addition, ex vivo converted DN T cells significantly inhibited the production of IgG by stimulated B cells. This study provides evidence that ex vivo converted CD4(-)CD8(-) double negative T cells can down-regulate immune responses by suppressing B cell proliferation and IgG production, and supports efforts to develop ex vivo DN T cell therapies.
authors.affiliation: Department of Hepatobiliary Surgery, Peking University People's Hospital, NO. 11, Xizhimen South Street, Xicheng District, Beijing 100044, China.
authors.forename: WenXia
authors.initials: W
authors.lastname: Li
authors.affiliation: Research Center, Beijing Friendship Hospital, Capital Medical University, No. 95, Yongan Road, Xicheng District, Beijing 100050, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Department of Hepatobiliary Surgery, Peking University People's Hospital, NO. 11, Xizhimen South Street, Xicheng District, Beijing 100044, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Hepatobiliary Surgery, Peking University People's Hospital, NO. 11, Xizhimen South Street, Xicheng District, Beijing 100044, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.affiliation: Research Center, Beijing Friendship Hospital, Capital Medical University, No. 95, Yongan Road, Xicheng District, Beijing 100050, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Shi
authors.affiliation: Department of Hepatobiliary Surgery, Peking University People's Hospital, NO. 11, Xizhimen South Street, Xicheng District, Beijing 100044, China. Electronic address: gandanwk@vip.sina.com.
authors.forename: JiYe
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Research Center, Beijing Friendship Hospital, Capital Medical University, No. 95, Yongan Road, Xicheng District, Beijing 100050, China. Electronic address: zhangd2010@hotmail.com.
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis; D001402:B-Lymphocytes / Q000276:immunology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D018345:Mice, Knockout; D052899:Pore Forming Cytotoxic Proteins / Q000235:genetics; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*; D019014:fas Receptor / Q000235:genetics
chemical_list: D007074:Immunoglobulin G; D052899:Pore Forming Cytotoxic Proteins; D019014:fas Receptor; C483886:perforin, mouse
keywords: B cells; CD4(−)CD8(−) double negative T cells; Immune suppression; Perforin