title: Marker assisted selection of low phytic acid trait in maize (Zea mays L.).
abstract: Maize is the third important major food crop. Breeding for low phytate maize genotypes is an effective strategy for decreasing the content of kernel phytic acid (a chelator of cations such as Ca(2+) and Fe(3+) ) and thereby increasing the bioavailability of nutritive minerals in human diet and animal feed. Previous studies have established that a mutant plant with a lpa2-2 allele accumulates less phytic acid in seeds. Therefore, the marker assisted backcross breeding (MABB), which involves introgression of lpa2-2 recessive allele (which confer low phytate trait) from a lpa2-2 mutant line into a well-adapted line using backcrosses and selection of lines possessing lpa2-2 allele in each backcross population using molecular markers, is an effective strategy for developing low phytate maize. So far, no studies have developed any lpa2-2 allele specific molecular markers for this purpose. Here, using backcross and selfed progenies, obtained by crossing low phytate mutant line 'EC 659418' (i.e. donor of lpa2-2 allele) into agronomically superior line 'UMI395', we have validated that a SSR marker 'umc2230', located 0.4 cM downstream of lpa2-2, cosegregate, in a Mendelian fashion, with low phytic acid trait. Therefore umc2230 can be dependably used in MABB for the development of low phytate maize.
authors.affiliation: Department of Plant biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamilnadu, IndiaDepartment of seed science and technology, Tamil Nadu Agricultural University, Coimbatore, Tamilnadu, IndiaDepartment of plant molecular biology and bioinformatics, Tamil Nadu Agricultural University, Coimbatore, Tamilnadu, IndiaCenter for plant breeding and genetics, Tamil Nadu Agricultural University, Coimbatore, Tamilnadu, IndiaDepartment of Veterinary and Animal Sciences, Tamil Nadu Agricultural University, Coimbatore, Tamilnadu, India.
authors.forename: S
authors.initials: S
authors.lastname: Sureshkumar
authors.forename: P
authors.initials: P
authors.lastname: Tamilkumar
authors.forename: N
authors.initials: N
authors.lastname: Senthil
authors.forename: P
authors.initials: P
authors.lastname: Nagarajan
authors.forename: A U
authors.initials: AU
authors.lastname: Thangavelu
authors.forename: M
authors.initials: M
authors.lastname: Raveendran
authors.forename: S
authors.initials: S
authors.lastname: Vellaikumar
authors.forename: K N
authors.initials: KN
authors.lastname: Ganesan
authors.forename: R
authors.initials: R
authors.lastname: Balagopal
authors.forename: G
authors.initials: G
authors.lastname: Vijayalakshmi
authors.forename: V
authors.initials: V
authors.lastname: Shobana
mesh_terms: D000483:Alleles; D001947:Breeding; D002118:Calcium / Q000378:metabolism; D005819:Genetic Markers; D006801:Humans; D007501:Iron / Q000378:metabolism; D010833:Phytic Acid / Q000378:metabolism*; D010940:Plant Proteins; D012641:Selection, Genetic; D003313:Zea mays / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D010940:Plant Proteins; D010833:Phytic Acid; D007501:Iron; D002118:Calcium