title: Treatment of rats with a self-selected hyperlipidic diet, increases the lipid content of the main adipose tissue sites in a proportion similar to that of the lipids in the rest of organs and tissues.
abstract: Adipose tissue (AT) is distributed as large differentiated masses, and smaller depots covering vessels, and organs, as well as interspersed within them. The differences between types and size of cells makes AT one of the most disperse and complex organs. Lipid storage is partly shared by other tissues such as muscle and liver. We intended to obtain an approximate estimation of the size of lipid reserves stored outside the main fat depots. Both male and female rats were made overweight by 4-weeks feeding of a cafeteria diet. Total lipid content was analyzed in brain, liver, gastrocnemius muscle, four white AT sites: subcutaneous, perigonadal, retroperitoneal and mesenteric, two brown AT sites (interscapular and perirenal) and in a pool of the rest of organs and tissues (after discarding gut contents). Organ lipid content was estimated and tabulated for each individual rat. Food intake was measured daily. There was a surprisingly high proportion of lipid not accounted for by the main macroscopic AT sites, even when brain, liver and BAT main sites were discounted. Muscle contained about 8% of body lipids, liver 1-1.4%, four white AT sites lipid 28-63% of body lipid, and the rest of the body (including muscle) 38-44%. There was a good correlation between AT lipid and body lipid, but lipid in "other organs" was highly correlated too with body lipid. Brain lipid was not. Irrespective of dietary intake, accumulation of body fat was uniform both for the main lipid storage and handling organs: large masses of AT (but also liver, muscle), as well as in the "rest" of tissues. These storage sites, in specialized (adipose) or not-specialized (liver, muscle) tissues reacted in parallel against a hyperlipidic diet challenge. We postulate that body lipid stores are handled and regulated coordinately, with a more centralized and overall mechanisms than usually assumed.
authors.affiliation: Department of Nutrition and Food Science, Faculty of Biology, University of Barcelona, Barcelona, Spain; Institute of Biomedicine, University of Barcelona, Barcelona, Spain; CIBER (Centro de Investigación Biomédica en Red) OBN (Obesidad y Nutrición), Institute of Health Carlos III, Madrid, Spain.
authors.forename: María Del Mar
authors.initials: Mdel M
authors.lastname: Romero
authors.affiliation: Department of Nutrition and Food Science, Faculty of Biology, University of Barcelona, Barcelona, Spain.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Roy
authors.affiliation: Department of Nutrition and Food Science, Faculty of Biology, University of Barcelona, Barcelona, Spain.
authors.forename: Karl
authors.initials: K
authors.lastname: Pouillot
authors.affiliation: Department of Nutrition and Food Science, Faculty of Biology, University of Barcelona, Barcelona, Spain.
authors.forename: Marisol
authors.initials: M
authors.lastname: Feito
authors.affiliation: Department of Nutrition and Food Science, Faculty of Biology, University of Barcelona, Barcelona, Spain; Institute of Biomedicine, University of Barcelona, Barcelona, Spain; CIBER (Centro de Investigación Biomédica en Red) OBN (Obesidad y Nutrición), Institute of Health Carlos III, Madrid, Spain.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Esteve
authors.affiliation: Department of Nutrition and Food Science, Faculty of Biology, University of Barcelona, Barcelona, Spain; Institute of Biomedicine, University of Barcelona, Barcelona, Spain; CIBER (Centro de Investigación Biomédica en Red) OBN (Obesidad y Nutrición), Institute of Health Carlos III, Madrid, Spain.
authors.forename: María Del Mar
authors.initials: Mdel M
authors.lastname: Grasa
authors.affiliation: Department of Nutrition and Food Science, Faculty of Biology, University of Barcelona, Barcelona, Spain; Institute of Biomedicine, University of Barcelona, Barcelona, Spain; CIBER (Centro de Investigación Biomédica en Red) OBN (Obesidad y Nutrición), Institute of Health Carlos III, Madrid, Spain.
authors.forename: José-Antonio
authors.initials: JA
authors.lastname: Fernández-López
authors.affiliation: Department of Nutrition and Food Science, Faculty of Biology, University of Barcelona, Barcelona, Spain; Institute of Biomedicine, University of Barcelona, Barcelona, Spain; CIBER (Centro de Investigación Biomédica en Red) OBN (Obesidad y Nutrición), Institute of Health Carlos III, Madrid, Spain.
authors.forename: Marià
authors.initials: M
authors.lastname: Alemany
authors.affiliation: Department of Nutrition and Food Science, Faculty of Biology, University of Barcelona, Barcelona, Spain; Institute of Biomedicine, University of Barcelona, Barcelona, Spain; CIBER (Centro de Investigación Biomédica en Red) OBN (Obesidad y Nutrición), Institute of Health Carlos III, Madrid, Spain.
authors.forename: Xavier
authors.initials: X
authors.lastname: Remesar
mesh_terms: D002001:Adipose Tissue, Brown / Q000737:chemistry / Q000378:metabolism*; D052436:Adipose Tissue, White / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D050218:Body Fat Distribution; D001835:Body Weight; D001921:Brain / Q000378:metabolism*; D059305:Diet, High-Fat; D004041:Dietary Fats / Q000009:adverse effects; D004435:Eating; D005260:Female; D006949:Hyperlipidemias / Q000209:etiology / Q000378:metabolism*; D050356:Lipid Metabolism; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism*; D009765:Obesity / Q000209:etiology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004041:Dietary Fats