title: Zinc transporter-1 concentrates at the postsynaptic density of hippocampal synapses.
abstract: BACKGROUND
  Zinc concentrates at excitatory synapses, both at the postsynaptic density and in a subset of glutamatergic boutons. Zinc can modulate synaptic plasticity, memory formation and nociception by regulating transmitter receptors and signal transduction pathways. Also, intracellular zinc accumulation is a hallmark of degenerating neurons in several neurological disorders. To date, no single zinc extrusion mechanism has been directly localized to synapses. Based on the presence of a canonical PDZ I motif in the Zinc Transporter-1 protein (ZnT1), we hypothesized that ZnT1 may be targeted to synaptic compartments for local control of cytosolic zinc. Using our previously developed protocol for the co-localization of reactive zinc and synaptic proteins, we further asked if ZnT1 expression correlates with presynaptic zinc content in individual synapses.
  
  
  RESULTS
  Here we demonstrate that ZnT1 is a plasma membrane protein that is enriched in dendritic spines and in biochemically isolated synaptic membranes. Hippocampal CA1 synapses labelled by postembedding immunogold showed over a 5-fold increase in ZnT1 concentration at synaptic junctions compared with extrasynaptic membranes. Subsynaptic analysis revealed a peak ZnT1 density on the postsynaptic side of the synapse, < 10 nm away from the postsynaptic membrane. ZnT1 was found in the vast majority of excitatory synapses regardless of the presence of vesicular zinc in presynaptic boutons.
  
  
  CONCLUSIONS
  Our study has identified ZnT1 as a novel postsynaptic density protein, and it may help elucidate the role of zinc homeostasis in synaptic function and disease.
authors.affiliation: Department of Pharmacology, University of Barcelona, Barcelona 08036, Spain. csindreu@ub.edu.
authors.forename: Carlos
authors.initials: C
authors.lastname: Sindreu
authors.forename: Álex
authors.initials: Á
authors.lastname: Bayés
authors.forename: Xavier
authors.initials: X
authors.lastname: Altafaj
authors.forename: Jeús
authors.initials: J
authors.lastname: Pérez-Clausell
mesh_terms: D000818:Animals; D027682:Cation Transport Proteins / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D057907:Post-Synaptic Density / Q000378:metabolism* / Q000648:ultrastructure; D013569:Synapses / Q000378:metabolism* / Q000648:ultrastructure; D013572:Synaptic Vesicles / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D027682:Cation Transport Proteins; C485803:Slc30a1 protein, mouse