title: Demonstration of metabolic and cellular effects of portal vein ligation using multi-modal PET/MRI measurements in healthy rat liver.
abstract: OBJECTIVE
  In the early recognition of portal vein ligation (PVL) induced tumor progression, positron emission tomography and magnetic resonance imaging (PET/MRI) could improve diagnostic accuracy of conventionally used methods. It is unknown how PVL affects metabolic patterns of tumor free hepatic tissues. The aim of this preliminary study is to evaluate the effect of PVL on glucose metabolism, using PET/MRI imaging in healthy rat liver.
  
  
  METHODS
  Male Wistar rats (n=30) underwent PVL. 2-deoxy-2-(18F)fluoro-D-glucose (FDG) PET/MRI imaging (nanoScan PET/MRI) and morphological/histological examination were performed before (Day 0) and 1, 2, 3, and 7 days after PVL. Dynamic PET data were collected and the standardized uptake values (SUV) for ligated and non-ligated liver lobes were calculated in relation to cardiac left ventricle (SUVVOI/SUVCLV) and mean liver SUV (SUVVOI/SUVLiver).
  
  
  RESULTS
  PVL induced atrophy of ligated lobes, while non-ligated liver tissue showed compensatory hypertrophy. Dynamic PET scan revealed altered FDG kinetics in both ligated and non-ligated liver lobes. SUVVOI/SUVCLV significantly increased in both groups of lobes, with a maximal value at the 2nd postoperative day and returned near to the baseline 7 days after the ligation. After PVL, ligated liver lobes showed significantly higher tracer uptake compared to the non-ligated lobes (significantly higher SUVVOI/SUVLiver values were observed at postoperative day 1, 2 and 3). The homogenous tracer biodistribution observed before PVL reappeared by 7th postoperative day.
  
  
  CONCLUSIONS
  The observed alterations in FDG uptake dynamics should be taken into account during the assessment of PET data until the PVL induced atrophic and regenerative processes are completed.
authors.affiliation: 1st Department of Surgery, Semmelweis University, Budapest, Hungary.
authors.forename: András
authors.initials: A
authors.lastname: Fülöp
authors.affiliation: 1st Department of Surgery, Semmelweis University, Budapest, Hungary.
authors.forename: Attila
authors.initials: A
authors.lastname: Szijártó
authors.affiliation: 1st Department of Surgery, Semmelweis University, Budapest, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Harsányi
authors.affiliation: 1st Department of Surgery, Semmelweis University, Budapest, Hungary.
authors.forename: András
authors.initials: A
authors.lastname: Budai
authors.affiliation: 1st Department of Surgery, Semmelweis University, Budapest, Hungary.
authors.forename: Damján
authors.initials: D
authors.lastname: Pekli
authors.affiliation: 1st Department of Surgery, Semmelweis University, Budapest, Hungary.
authors.forename: Diána
authors.initials: D
authors.lastname: Korsós
authors.affiliation: Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary.
authors.forename: Ildikó
authors.initials: I
authors.lastname: Horváth
authors.affiliation: CROmed Translational Research Centers, Budapest, Hungary.
authors.forename: Noémi
authors.initials: N
authors.lastname: Kovács
authors.affiliation: Department of Radiology and Oncotherapy, Semmelweis University, Budapest, Hungary.
authors.forename: Kinga
authors.initials: K
authors.lastname: Karlinger
authors.affiliation: CROmed Translational Research Centers, Budapest, Hungary.
authors.forename: Domokos
authors.initials: D
authors.lastname: Máthé
authors.affiliation: Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary.
authors.forename: Krisztián
authors.initials: K
authors.lastname: Szigeti
mesh_terms: D000818:Animals; D001284:Atrophy; D019788:Fluorodeoxyglucose F18; D006003:Glycogen / Q000378:metabolism; D006262:Health; D006352:Heart Ventricles / Q000000981:diagnostic imaging; D006984:Hypertrophy; D008026:Ligation / Q000379:methods*; D008099:Liver / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000473:pathology*; D008279:Magnetic Resonance Imaging; D008297:Male; D064847:Multimodal Imaging; D011169:Portal Vein / Q000601:surgery*; D049268:Positron-Emission Tomography; D011849:Radioactive Tracers; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014018:Tissue Distribution
chemical_list: D011849:Radioactive Tracers; D019788:Fluorodeoxyglucose F18; D006003:Glycogen