title: Enhanced production of cellulose degrading CMCase by newly isolated strain of Aspergillus versicolor.
abstract: Production of CMCase from newly isolated strain of Aspergillus versicolor was studied by optimizing different physico-chemical parameters. Cellulolytic potential of the isolate was confirmed by the simple agar plate method and then subjected to variation of a single parameter at a time in CMC containing medium. Maximum enzyme production was obtained at 30°C in 120 h when medium was supplemented with 0.5 gm% carboxymethyl cellulose, 0.2 gm% Tween 80, 0.075 gm% peptone, 0.050 gm% CaCl2, 1.5 gm% NaNO3 and 0.2 gm% KH2PO4 at pH 4.0. Current study holds great potential as the organism reported here could easily be used for the production of cellulase using renewable biomass.
authors.affiliation: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi 75270, Pakistan.
authors.forename: Sofia
authors.initials: S
authors.lastname: Qaisar
authors.affiliation: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi 75270, Pakistan.
authors.forename: Rashida Rahmat
authors.initials: RR
authors.lastname: Zohra
authors.affiliation: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi 75270, Pakistan. Electronic address: afsheen93@yahoo.com.
authors.forename: Afsheen
authors.initials: A
authors.lastname: Aman
authors.affiliation: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi 75270, Pakistan.
authors.forename: Shah Ali Ul
authors.initials: SA
authors.lastname: Qader
mesh_terms: D001230:Aspergillus / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D002266:Carboxymethylcellulose Sodium / Q000378:metabolism*; D002480:Cellulase / Q000378:metabolism*; D005285:Fermentation; D005656:Fungal Proteins / Q000378:metabolism*; D007218:Industrial Microbiology
chemical_list: D005656:Fungal Proteins; D002480:Cellulase; D002266:Carboxymethylcellulose Sodium
keywords: Aspergillus versicolor; CMCase; Carboxymethyl cellulose; Fermentation