title: The complete genome sequence of the Alphaentomopoxvirus Anomala cuprea entomopoxvirus, including its terminal hairpin loop sequences, suggests a potentially unique mode of apoptosis inhibition and mode of DNA replication.
abstract: Complete genome sequence of Anomala cuprea entomopoxvirus, which belongs to the genus Alphaentomopoxvirus, including its terminal hairpin loop sequences, is reported. This is the first genome sequence of Alphaentomopoxvirus reported, and hairpin loops in entomopoxviruses have not previously been sequenced. The genome is 245,717 bp, which is smaller than had previously been estimated for Alphaentomopoxvirus. The inverted terminal repeats are quite long, and experimental results suggest that one genome molecule has one type of hairpin at one end and another type at the other end. The genome contains unexpected ORFs, e.g., that for the ubiquitin-conjugating enzyme E2 of eukaryotes. The BIR and RING domains found in a single ORF for an inhibitor of apoptosis in baculoviruses and entomopoxviruses occurred in two different, widely separated ORFs. Furthermore, an ORF in the genome contains a serpin domain that was previously found in vertebrate poxviruses for apoptosis inhibition but not in insect viruses.
authors.affiliation: National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8634, Japan. Electronic address: mitsuhas@affrc.go.jp.
authors.forename: Wataru
authors.initials: W
authors.lastname: Mitsuhashi
authors.affiliation: National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8634, Japan.
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Miyamoto
authors.affiliation: National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8634, Japan.
authors.forename: Sanae
authors.initials: S
authors.lastname: Wada
mesh_terms: D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D004261:DNA Replication; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017996:Entomopoxvirinae / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D016679:Genome, Viral; D007313:Insecta / Q000166:cytology* / Q000821:virology; D055029:Inverted Repeat Sequences; D008969:Molecular Sequence Data; D011213:Poxviridae Infections / Q000503:physiopathology / Q000662:veterinary* / Q000821:virology; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins
keywords: Alphaentomopoxvirus; Apoptosis inhibition; DNA replication; Entomopoxvirus; Genome; Terminal hairpin loop