title: Genetic diversity and population structure of black Dahe pig based on DNA sequences analyses of mitochondrial and nuclear genes.
abstract: To investigate the genetic diversity and population structure of black Dahe pigs, we collected 175 samples from 5 local populations and sequenced them using a combination of two selected molecular markers for mitochondrial cytochrome b and Major Histocompatibility Complex (MHC) DRB. Overall, the results of AMOVA and phylogenetic tree and gene flow analyses detected high levels of gene flow among the five populations, particularly individual pigs from Dahe town (Pop1) or Yingshang town (Pop2) to other populations (Pop3, Pop4, and Pop5). The genetic diversity analyses showed that the diversity indices of the five populations did not vary significantly, but they were much lower than those of other Chinese pig species. These results suggest that distinct gene flow, unstable population pattern, and lower genetic diversity have been influenced mainly by human introductions for economic ends. These findings provide genetic information that could be used for the preservation and further genetic improvement of the black Dahe pig, as well as an important reference for the evaluation, conservation, and utilization of the genetic resources of this breed.
authors.affiliation: a Key Laboratory of Molecular Biology , Institute of Biologic Resource and Environmental Science, Qujing Normal University , Qujing , Yunnan , China and.
authors.forename: Lizhou
authors.initials: L
authors.lastname: Tang
authors.affiliation: a Key Laboratory of Molecular Biology , Institute of Biologic Resource and Environmental Science, Qujing Normal University , Qujing , Yunnan , China and.
authors.forename: Long
authors.initials: L
authors.lastname: Yu
authors.affiliation: a Key Laboratory of Molecular Biology , Institute of Biologic Resource and Environmental Science, Qujing Normal University , Qujing , Yunnan , China and.
authors.forename: Junjie
authors.initials: J
authors.lastname: Wang
authors.affiliation: a Key Laboratory of Molecular Biology , Institute of Biologic Resource and Environmental Science, Qujing Normal University , Qujing , Yunnan , China and.
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.affiliation: a Key Laboratory of Molecular Biology , Institute of Biologic Resource and Environmental Science, Qujing Normal University , Qujing , Yunnan , China and.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Shi
authors.affiliation: a Key Laboratory of Molecular Biology , Institute of Biologic Resource and Environmental Science, Qujing Normal University , Qujing , Yunnan , China and.
authors.forename: Wei
authors.initials: W
authors.lastname: Ding
authors.affiliation: a Key Laboratory of Molecular Biology , Institute of Biologic Resource and Environmental Science, Qujing Normal University , Qujing , Yunnan , China and.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhu
authors.affiliation: b Key Laboratory of the Qinghai-Tibetan Plateau Ecosystem and Biological Evolution and Adaptation , Northwest Institute of Plateau Biology, Chinese Academy of Sciences , Xining , Qinghai , China.
authors.forename: Songchang
authors.initials: S
authors.lastname: Guo
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000235:genetics*; D002681:China; D045303:Cytochromes b / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics; D051456:Gene Flow; D050259:Genes, Mitochondrial; D014644:Genetic Variation; D005828:Genetics, Population; D008285:Major Histocompatibility Complex; D010802:Phylogeny; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013552:Swine / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial; D045303:Cytochromes b
keywords: Black Dahe pig; Major Histocompatibility Complex; cytochrome b gene; genetic polymorphism; population genetics