title: Characterization of a new wheat-Aegilops biuncialis addition line conferring quality-associated HMW glutenin subunits.
abstract: In this study, a new disomic addition line, 12-5-2, with 44 chromosomes that was derived from BC3F2 descendants of the hybridization between Triticum aestivum cv. CN19 and Aegilops biuncialis was created and reported. 12-5-2 was immune to both powdery mildew and stripe rust and has stable fertility. Fluorescence in situ hybridization and C-banding revealed that 12-5-2 was a 1U(b) disomic addition line (ADL1U(b)). The seed storage protein electrophoresis showed that 12-5-2 presented all high molecular weight glutenin subunits (7 + 8 and 2 + 12) of CN19 and 2 new subunits that were designated Ux and Uy. Additionally, the flour quality parameters showed that the protein content, Zeleny sedimentation value, wet gluten content, and grain hardness of 12-5-2 were significantly higher than those of its parent CN19. Moreover, 5 pairs of the chromosome 1U(b)-specific polymerase chain reaction-based landmark unique gene markers, TNAC1021, TNAC1041, TNAC1071, TNAC1-01, and TNAC1-04, were also obtained. The new ADL1U(b) 12-5-2 could be a valuable source for wheat improvement, especially for wheat end-product quality and resistance to disease.
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan, People's Republic of China zhoujp@uestc.edu.cn.
authors.forename: J P
authors.initials: JP
authors.lastname: Zhou
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan, People's Republic of China.
authors.forename: C H
authors.initials: CH
authors.lastname: Yao
authors.affiliation: Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan, People's Republic of China.
authors.forename: E N
authors.initials: EN
authors.lastname: Yang
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan, People's Republic of China.
authors.forename: M Q
authors.initials: MQ
authors.lastname: Yin
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan, People's Republic of China.
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan, People's Republic of China.
authors.forename: Z L
authors.initials: ZL
authors.lastname: Ren
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics*; D002523:Edible Grain / Q000235:genetics; D005983:Glutens / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D006824:Hybridization, Genetic; D017404:In Situ Hybridization, Fluorescence; D021122:Protein Subunits / Q000235:genetics; D014908:Triticum / Q000235:genetics*
chemical_list: D021122:Protein Subunits; D005983:Glutens; C037302:glutenin