title: Dual genetically encoded phage-displayed ligands.
abstract: M13 bacteriophage display presents polypeptides as fusions to phage coat proteins. Such phage-displayed ligands offer useful reagents for biosensors. Here, we report a modified phage propagation protocol for the consistent and robust display of two different genetically encoded ligands on the major coat protein, P8. The results demonstrate that the phage surface reaches a saturation point for maximum peptide display.
authors.affiliation: Department of Chemistry, University of California, Irvine, Irvine, CA 92697, USA.
authors.forename: Kritika
authors.initials: K
authors.lastname: Mohan
authors.affiliation: Department of Chemistry, University of California, Irvine, Irvine, CA 92697, USA; Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA 92697, USA. Electronic address: gweiss@uci.edu.
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Weiss
mesh_terms: D017104:Bacteriophage M13 / Q000235:genetics*; D036022:Capsid Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008024:Ligands; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D013499:Surface Properties
chemical_list: D036022:Capsid Proteins; D008024:Ligands; D010455:Peptides
keywords: Bacterial transformation; ELISA; Phage; Phage display