title: One-step separation of four flavonoids from Herba Salviae Plbeiae by HSCCC.
abstract: High-speed countercurrent chromatography (HSCCC) was successfully applied to the isolation and purification of four flavonoids from Herba Salviae Plbeiae using stepwise and dual-mode elution with a pair of two-phase solvent systems. The systems composed of n-hexane-chloroform-methanol-water (0.5 : 4 : 3 : 2, v/v/v/v) and chloroform-methanol-water (4 : 3 : 2, v/v/v). Analytical HSCCC was used for the preliminary selection of a suitable system, and a linear scale-step procedure was performed at a preparative grade. Hispidulin (91 mg), nepetin (148 mg), homoplantaginin (405 mg) and nepetin-7-glucoside (192 mg) could be obtained from 1,500-mg crude sample in a one-step separation, with purities >95% as determined by high-performance liquid chromatography. Their chemical structures were identified by (1)H nuclear magnetic resonance (NMR) and (13)C NMR.
authors.affiliation: Laboratory of Oral Biomedical Science and Translational Medicine, School of Stomatology, Tongji University, Shanghai 200072, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.affiliation: Research and Development Department, Shanghai Tauto Biotech Co., Ltd, No. 326 Edison Road, Zhangjiang High-Tech Park, Shanghai 201203, China.
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Research and Development Department, Shanghai Tauto Biotech Co., Ltd, No. 326 Edison Road, Zhangjiang High-Tech Park, Shanghai 201203, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Research and Development Department, Shanghai Tauto Biotech Co., Ltd, No. 326 Edison Road, Zhangjiang High-Tech Park, Shanghai 201203, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Fu
authors.affiliation: Research and Development Department, Shanghai Tauto Biotech Co., Ltd, No. 326 Edison Road, Zhangjiang High-Tech Park, Shanghai 201203, China wwna2013@sina.com.
authors.forename: Weina
authors.initials: W
authors.lastname: Wang
mesh_terms: D002725:Chloroform; D003377:Countercurrent Distribution / Q000379:methods*; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D006586:Hexanes; D000432:Methanol; D010936:Plant Extracts / Q000737:chemistry*; D027544:Salvia / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D006586:Hexanes; D010936:Plant Extracts; C026385:n-hexane; D002725:Chloroform; D000432:Methanol