title: Elevated expression level of microRNA-196a is predictive of intestinal-type intraductal papillary mucinous neoplasm of the pancreas.
abstract: OBJECTIVE
  Aberrant expression of several microRNAs (miRs) has been reported in various neoplasms including intraductal papillary mucinous neoplasms (IPMNs) of the pancreas. MicroRNA-196a (miR-196a) is up-regulated in Barrett esophagus (characterized by intestinal metaplasia) and in colorectal cancer; this relationship between intestinal characteristics and miR-196a might also be applicable to intestinal-type IPMNs. The aim of this study was to evaluate whether intestinal-type IPMNs can be discriminated from non-intestinal-type IPMNs by the expression level of miR-196a in tissue and pancreatic juice samples.
  
  
  METHODS
  Thirty-seven formalin-fixed paraffin-embedded tissue samples (including 3 of normal pancreatic ducts) and 36 pancreatic juice samples were obtained. The expression level of miR-196a measured by quantitative reverse transcription-polymerase chain reaction assays was compared between intestinal-type and non-intestinal-type IPMNs.
  
  
  RESULTS
  MicroRNA-196a expression in intestinal-type IPMN tissue samples (n = 18) was significantly higher than that of non-intestinal-type IPMNs (n = 16) (P < 0.001). Similarly, miR-196a expression in pancreatic juice samples of intestinal-type IPMNs (n = 6) was significantly higher than that of non-intestinal-type IPMNs (n = 30) (P = 0.008), and the sensitivity and specificity for prediction of intestinal-type IPMNs using pancreatic juice samples were both 83%.
  
  
  CONCLUSIONS
  Elevated expression of miR-196a in pancreatic juice samples is predictive of intestinal-type IPMNs.
authors.affiliation: From the Departments of *Surgery and Oncology, and †Anatomic Pathology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Teppei
authors.initials: T
authors.lastname: Aso
authors.forename: Takao
authors.initials: T
authors.lastname: Ohtsuka
authors.forename: Koji
authors.initials: K
authors.lastname: Tamura
authors.forename: Noboru
authors.initials: N
authors.lastname: Ideno
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kono
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Nagayoshi
authors.forename: Kennoki
authors.initials: K
authors.lastname: Ohuchida
authors.forename: Junji
authors.initials: J
authors.lastname: Ueda
authors.forename: Shunnichi
authors.initials: S
authors.lastname: Takahata
authors.forename: Koji
authors.initials: K
authors.lastname: Shindo
authors.forename: Shinichi
authors.initials: S
authors.lastname: Aishima
authors.forename: Yoshinao
authors.initials: Y
authors.lastname: Oda
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Mizumoto
authors.forename: Masao
authors.initials: M
authors.lastname: Tanaka
mesh_terms: D002288:Adenocarcinoma, Mucinous / Q000175:diagnosis / Q000235:genetics*; D000231:Adenocarcinoma, Papillary / Q000175:diagnosis / Q000235:genetics*; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics; D021441:Carcinoma, Pancreatic Ductal / Q000175:diagnosis / Q000235:genetics*; D003937:Diagnosis, Differential; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007422:Intestines / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D010189:Pancreatic Juice / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000175:diagnosis / Q000235:genetics*; D011237:Predictive Value of Tests; D011379:Prognosis; D012372:ROC Curve; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; C542543:MIRN196 microRNA, human; D035683:MicroRNAs