title: [Application of multiplex PCR for analysis of polycomponent probiotic preparations].
abstract: OBJECTIVE
  Development of multiplex PCR for indication and differential analysis of CFU in a probiotic preparation containing 3 various species ofbifidobacteria (Bifidobacterium bifidum, B. longum, B. adolescentis) and 2 species of lactobacilli (Lactobacillus acidophilus, L. plantarum). MATERIALS AND METHODS; Multiplex PCR with primers to 16S RNA genes of 5 species of bacteria was applied. Samples of bacterial colonies were removed from solid nutrient medium and transferred into test-tube for multiplex PCR execution. Bacterial lysates were used as target-matrix for amplification and determination of presence of the required amplicons.
  
  
  RESULTS
  Multiplex PCR for detection of CFU of each of the bifidobacteria and lactobacilli strains in samples of 5-component probiotic with accuracy up to species was developed. A reagent kit was fine-tuned and high reproducibility and specificity of the multiplex PCR method with the accuracy of 90 - 95% of cases was shown.
  
  
  CONCLUSIONS
  The multiplex PCR method developed is recommended to be applied for CFU control of each bacterial component in a complex probiotic preparation.
authors.forename: I I
authors.initials: II
authors.lastname: Vaĭnshtok
authors.forename: I Iu
authors.initials: IIu
authors.lastname: Shchit
authors.forename: V M
authors.initials: VM
authors.lastname: Bondarenko
authors.forename: I B
authors.initials: IB
authors.lastname: Brodskiĭ
mesh_terms: D001644:Bifidobacterium / Q000235:genetics*; D007778:Lactobacillus / Q000235:genetics*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D019936:Probiotics