title: Effects of some pesticides on development of Ascaris suum eggs.
abstract: To evaluate the effects of pesticides to parasite eggs, Ascaris suum eggs were incubated with 5 different pesticides (1:1,500-1:2,000 dilutions of 2% emamectin benzoate, 5% spinetoram, 5% indoxacarb, 1% deltamethrin, and 5% flufenoxuron; all v/v) at 20℃ for 6 weeks, and microscopically evaluated the egg survival and development on a weekly basis. The survival rate of A. suum eggs incubated in normal saline (control eggs) was 90±3% at 6 weeks. However, the survival rates of eggs treated with pesticides were 75-85% at this time, thus significantly lower than the control value. Larval development in control eggs commenced at 3 weeks, and 73±3% of eggs had internal larvae at 6 weeks. Larvae were evident in pesticide-treated eggs at 3-4 weeks, and the proportions of eggs carrying larvae at 6 weeks (36±3%-54±3%) were significantly lower than that of the control group. Thus, pesticides tested at levels similar to those used in agricultural practices exhibited low-level ovicidal activity and delayed embryogenesis of A. suum eggs, although some differences were evident among the tested pesticides.
authors.affiliation: Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Yong-Man
authors.initials: YM
authors.lastname: Yu
authors.affiliation: Department of Environmental Horticulture, The University of Seoul, Seoul 130-743, Korea.
authors.forename: Jin-Won
authors.initials: JW
authors.lastname: Kim
authors.affiliation: Department of Environmental Horticulture, The University of Seoul, Seoul 130-743, Korea.
authors.forename: Won-Seok
authors.initials: WS
authors.lastname: Na
authors.affiliation: Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Young-Nam
authors.initials: YN
authors.lastname: Youn
authors.affiliation: Department of Infection Biology, Chungnam National University School of Medicine, Daejeon 301-131, Korea.
authors.forename: In-Wook
authors.initials: IW
authors.lastname: Choi
authors.affiliation: Department of Infection Biology, Chungnam National University School of Medicine, Daejeon 301-131, Korea.
authors.forename: Young-Ha
authors.initials: YH
authors.lastname: Lee
mesh_terms: D000818:Animals; D017165:Ascaris suum / Q000187:drug effects* / Q000254:growth & development; D005260:Female; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D008853:Microscopy; D010575:Pesticides / Q000494:pharmacology*; D016019:Survival Analysis; D013696:Temperature; D013995:Time; D015053:Zygote / Q000187:drug effects* / Q000254:growth & development
chemical_list: D010575:Pesticides
keywords: Ascaris suum; egg; embryogenesis; larval development; pesticide