title: Molecular characterization of the small nonstructural proteins of parvovirus Aleutian mink disease virus (AMDV) during infection.
abstract: Aleutian mink disease virus (AMDV) is the only member in genus Amdovirus of the family Parvoviridae. During AMDV infection, six species of viral transcripts are generated from one precursor mRNA through alternative splicing and alternative polyadenylation. In addition to the large non-structural protein NS1, two small non-structural proteins, NS2 and NS3, are putatively encoded (Qiu J, et al., 2006. J. Virol. 80 654-662). However, these two proteins have not been experimentally demonstrated during virus infection, and nothing is known about their function. Here, we studied the nonstructural protein expression profile of AMDV, and for the first time, confirmed expression of NS2 and NS3 during infection, and identified their intracellular localization. More importantly, we provided evidence that both NS2 and NS3 are necessary for AMDV replication.
authors.affiliation: Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, United States.
authors.forename: Qinfeng
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, United States.
authors.forename: Yong
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, United States.
authors.forename: Fang
authors.initials: F
authors.lastname: Cheng
authors.affiliation: Laboratory of Virology, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, MT, United States.
authors.forename: Sonja M
authors.initials: SM
authors.lastname: Best
authors.affiliation: Laboratory of Virology, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, MT, United States.
authors.forename: Marshall E
authors.initials: ME
authors.lastname: Bloom
authors.affiliation: Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, United States. Electronic address: jqiu@kumc.edu.
authors.forename: Jianming
authors.initials: J
authors.lastname: Qiu
mesh_terms: D000453:Aleutian Mink Disease / Q000821:virology*; D000454:Aleutian Mink Disease Virus / Q000235:genetics / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D008907:Mink; D008969:Molecular Sequence Data; D021381:Protein Transport; D016415:Sequence Alignment; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D017361:Viral Nonstructural Proteins
keywords: Aleutian mink disease virus; DNA replication; Non-structural protein; Parvovirus