title: [Application of high resolution HLA typing in unrelated umbilical cord blood transplantation].
abstract: This study was purposed to investigate the clinical value of HLA matching(low and high resolution) and its effect on outcome of the patients received umbilical cord blood transplantation(UCBT). Sequence-specific oligonucleotide probe (SSOP) , sequence-based typing (SBT) and sequence-specific primers(SSP) were used to perform high resolution HLA matching for HLA-A, -B, -Cw, -DRB1, -DQB1 and low resolution for HLA-A, B, DRB1 among 34 patients with hematologic malignancies who received unrelated UCB transplantation and grafts. The effects of HLA matching (low or high resolution ) on leading engraftment, hematopoietic reconstitution, graft-versus-host disease (GVHD) and infection after UCB transplantation were analyzed by comparison. The results showed that the median of total nucleated cells (TNC) of transplanted cord blood was 6.0×10(7)/kg, The time of neutrophil recovery was significantly shortened when more than 5×10(7)/kg TNC were transplanted (P < 0.05). The HLA-(6-10)/10 group of high resolution HLA matching was better than the HLA (4-5)/10 group in the respect of leading engraftment, the time of platelet recovery and the rate of acute GVHD (P < 0.05). In contrast, HLA-I+II locus, HLA-DRB1 or HLA-DQB1 locus mismatch could prolong the platelet engraftment time (P < 0.05). There was statistical difference in the time of platelet recovery, the rate of acute GVHD between the HLA (5-6)/6 group of low resolution HLA matching and the HLA (3-4)/6 group after UCB transplantation (P < 0.05), but the mismatch locus of HLA with low resolution did not correlate with the time of platelet recovery (P > 0.05). It is concluded that the high resolution HLA matching between patients received unrelated UCB transplantation and grafts may contribute to select the better UCB, that has important clinical value to promote hematopoietic reconstitution and to reduce the complications after UCB transplantation.
authors.affiliation: Jiangsu Instiute of Hematology, The First Affiliated Hospial of Soochow University; Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Suzhou 215006, Jiangsu Province, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Yang
authors.affiliation: Jiangsu Instiute of Hematology, The First Affiliated Hospial of Soochow University; Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Suzhou 215006, Jiangsu Province, China. E-mail:junhe1964@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: He
authors.affiliation: Jiangsu Instiute of Hematology, The First Affiliated Hospial of Soochow University; Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Suzhou 215006, Jiangsu Province, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Jiangsu Instiute of Hematology, The First Affiliated Hospial of Soochow University; Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Suzhou 215006, Jiangsu Province, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Xu
authors.affiliation: Jiangsu Instiute of Hematology, The First Affiliated Hospial of Soochow University; Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Suzhou 215006, Jiangsu Province, China.
authors.forename: Xiao-Jing
authors.initials: XJ
authors.lastname: Bao
authors.affiliation: Jiangsu Instiute of Hematology, The First Affiliated Hospial of Soochow University; Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Suzhou 215006, Jiangsu Province, China.
authors.forename: Xiao-Ni
authors.initials: XN
authors.lastname: Yuan
authors.affiliation: Jiangsu Instiute of Hematology, The First Affiliated Hospial of Soochow University; Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Suzhou 215006, Jiangsu Province, China.
authors.forename: Hui-Ying
authors.initials: HY
authors.lastname: Qiu
authors.affiliation: Jiangsu Instiute of Hematology, The First Affiliated Hospial of Soochow University; Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Suzhou 215006, Jiangsu Province, China.
authors.forename: Zheng-Ming
authors.initials: ZM
authors.lastname: Jin
authors.affiliation: Jiangsu Instiute of Hematology, The First Affiliated Hospial of Soochow University; Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Suzhou 215006, Jiangsu Province, China.
authors.forename: Xiao-Wen
authors.initials: XW
authors.lastname: Tang
authors.affiliation: Jiangsu Instiute of Hematology, The First Affiliated Hospial of Soochow University; Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Suzhou 215006, Jiangsu Province, China.
authors.forename: Cheng-Cheng
authors.initials: CC
authors.lastname: Fu
authors.affiliation: Jiangsu Instiute of Hematology, The First Affiliated Hospial of Soochow University; Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Suzhou 215006, Jiangsu Province, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Han
authors.affiliation: Jiangsu Instiute of Hematology, The First Affiliated Hospial of Soochow University; Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Suzhou 215006, Jiangsu Province, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Ma
authors.affiliation: Jiangsu Instiute of Hematology, The First Affiliated Hospial of Soochow University; Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Suzhou 215006, Jiangsu Province, China.
authors.forename: De-Pei
authors.initials: DP
authors.lastname: Wu
authors.affiliation: Jiangsu Instiute of Hematology, The First Affiliated Hospial of Soochow University; Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Suzhou 215006, Jiangsu Province, China. E-mail:ainingsun@hotmail.com.
authors.forename: Ai-Ning
authors.initials: AN
authors.lastname: Sun
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D036101:Cord Blood Stem Cell Transplantation / Q000379:methods; D005260:Female; D019337:Hematologic Neoplasms / Q000628:therapy*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D008297:Male; D055815:Young Adult