title: Post-translational regulation of SHORT VEGETATIVE PHASE as a major mechanism for thermoregulation of flowering.
abstract: In contrast to our extensive knowledge of vernalization, we know relatively little about the regulation of ambient temperature-responsive flowering. Recent reports revealed that flowering locus M (FLM) and short vegetative phase (SVP) regulate high ambient temperature-responsive flowering through two different mechanisms: degradation of SVP protein and formation of a non-functional SVP-FLM-δ complex. To investigate further the mechanism of thermoregulation of flowering, we performed real-time quantitative polymerase chain reaction (RT-qPCR) and in vitro pull-down assays. We found that FLM-β and FLM-δ transcripts show similar absolute levels at different temperatures. Also, His-SVP protein bound to the GST-FLM-β or -δ proteins with similar binding intensities. These results suggest that functional SVP-FLM-β and non-functional SVP-FLM-δ complexes form similarly at warmer temperatures, thus indicating that post-translational regulation of SVP functions as a major mechanism for thermoregulation in flowering.
authors.affiliation: Creative Research Initiatives; Department of Life Sciences; Korea University, Seoul, South Korea.
authors.forename: Jeong
authors.initials: J
authors.lastname: Hwan Lee
authors.affiliation: Creative Research Initiatives; Department of Life Sciences; Korea University, Seoul, South Korea.
authors.forename: Kyung
authors.initials: K
authors.lastname: Sook Chung
authors.affiliation: Creative Research Initiatives; Department of Life Sciences; Korea University, Seoul, South Korea.
authors.forename: Soon-Kap
authors.initials: SK
authors.lastname: Kim
authors.affiliation: Creative Research Initiatives; Department of Life Sciences; Korea University, Seoul, South Korea.
authors.forename: Ji Hoon
authors.initials: JH
authors.lastname: Ahn
mesh_terms: D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D035264:Flowers / Q000502:physiology*; D011499:Protein Processing, Post-Translational; D013696:Temperature; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C407549:SVP protein, Arabidopsis; D014157:Transcription Factors
keywords: Alternative splicing; Ambient temperature; Ambient temperature-responsive flowering; FLM-β; FLM-δ; SVP; thermoregulation