title: Optimization of extraction process and antioxidant activity of polysaccharides from leaves of Paris polyphylla.
abstract: Based on a single-factor test, a central composite design was used to optimize the extraction conditions of polysaccharides from leaves of Paris polyphylla Smith var. yunnanensis (Franch.) Hand.-Mazz. Three independent variables, including extraction temperature (°C), ratio of water to raw material, and extraction time (h), which significantly affected the yield of polysaccharides, were investigated. The experimental data were fitted to a quadratic polynomial equation using multiple regression analysis and also examined using appropriate statistical methods. The optimum conditions were as follows: extraction temperature, 90.8°C; ratio of water to raw material, 21.3:1; and extraction time 4.8h. Under these conditions, the experimental yield was 54.18%, which matched the predicted value well. Furthermore, the purified polysaccharide exerted strong antioxidant effects on DPPH, hydroxyl, and superoxide radicals in vitro.
authors.affiliation: College of Biology and Science, Sichuan Agricultural University, Yaan 625014, Sichuan, China.
authors.forename: Shian
authors.initials: S
authors.lastname: Shen
authors.affiliation: Institute of Biotechnology, Wenshan Academy of Agricultural Sciences, Wenshan 663000, Yunnan, China.
authors.forename: Daju
authors.initials: D
authors.lastname: Chen
authors.affiliation: College of Biology and Science, Sichuan Agricultural University, Yaan 625014, Sichuan, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Public Relations, Chengdu 611130, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Li
authors.affiliation: College of Biology and Science, Sichuan Agricultural University, Yaan 625014, Sichuan, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yuan
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Biology and Science, Sichuan Agricultural University, Yaan 625014, Sichuan, China. Electronic address: dcb@sicau.edu.cn.
authors.forename: Chunbang
authors.initials: C
authors.lastname: Ding
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D005591:Chemical Fractionation / Q000379:methods*; D003627:Data Interpretation, Statistical; D019668:Liliaceae / Q000033:anatomy & histology / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D011134:Polysaccharides
keywords: Antioxidant; Optimization; Paris polyphylla; Polysaccharide