title: Effects of sub-chronic aluminum chloride on spermatogenesis and testicular enzymatic activity in male rats.
abstract: OBJECTIVE
  The aim of this study was to determine the effects of sub-chronic aluminum chloride (AlCl3) on spermatogenesis and testicular enzymatic activity in male rats.
  
  
  METHODS
  Forty Wistar male rats were randomly divided into four groups: control group (CG, 0), low-dose group (LG, 64.18 mg/kg BW AlCl3), mid-dose group (MG, 128.36 mg/kg BW AlCl3) and high-dose group (HG, 256.72 mg/kg BW AlCl3). The rats were orally administered with AlCl3 for 120 days. At the end of the experiment, the contents of Al, Fe, Cu and Zn, the enzyme activities of testicular acid phosphatase (ACP), succinate dehydrogenase (SDH), lactate dehydrogenase (LDH), lactate dehydrogenase isoenzyme (LDH-x), the sperm count and the sperm malformation rate were examined.
  
  
  RESULTS
  The results showed that the Al and Cu contents, sperm count and the enzyme activities of testicular ACP, SDH, LDH and LDH-x decreased, while the Zn and Fe contents and sperm malformation rate increased in AlCl3-treated rats.
  
  
  CONCLUSIONS
  It suggests that sub-chronic AlCl3 disorders the balance of trace element and decreases the spermatogenesis and the activities of testicular enzymes, indicating that AlCl3 has adverse effect on the testicular function in male rats.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun 130112, China.
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Zhu
authors.affiliation: Laoshan Agricultural Bureau Institute of Animal Health Supervision, Yantai 264003, China.
authors.forename: H
authors.initials: H
authors.lastname: Sun
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Fu
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors.forename: M
authors.initials: M
authors.lastname: Song
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China. Electronic address: yanfeili200@126.com.
authors.forename: Y F
authors.initials: YF
authors.lastname: Li
authors.affiliation: Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun 130112, China. Electronic address: mliguang66@sohu.com.
authors.forename: L G
authors.initials: LG
authors.lastname: Miao
mesh_terms: D000284:Administration, Oral; D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000008:administration & dosage / Q000633:toxicity*; D000818:Animals; D002712:Chlorides / Q000008:administration & dosage / Q000633:toxicity*; D003300:Copper / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D007501:Iron / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013076:Sperm Count; D013091:Spermatogenesis / Q000187:drug effects*; D013737:Testis / Q000187:drug effects* / Q000201:enzymology; D013997:Time Factors; D015032:Zinc / Q000378:metabolism
chemical_list: D017607:Aluminum Compounds; D002712:Chlorides; D000077410:Aluminum Chloride; D003300:Copper; D007501:Iron; D015032:Zinc
keywords: Aluminum chloride; Rat; Spermatogenesis; Testicular enzymes