title: Genetic modification of mouse effector and helper T lymphocytes expressing a chimeric antigen receptor.
abstract: Genetic modification of primary mouse T cells with chimeric antigen receptors (CAR) has emerged as an important tool for optimizing adoptive T cell immunotherapy strategies. However, limitations in current protocols for generating highly pure and sufficient numbers of enriched effector and helper CAR(+) T cell subsets remain problematic. Here, we describe a new retroviral transduction protocol for successfully generating transduced CD8(+) and CD4(+) T lymphocytes for in vitro and in vivo characterization.
authors.affiliation: Cancer Immunology Program, Peter MacCallum Cancer Centre, East Melbourne, VIC, Australia.
authors.forename: Liza B
authors.initials: LB
authors.lastname: John
authors.forename: Tess M
authors.initials: TM
authors.lastname: Chee
authors.forename: David E
authors.initials: DE
authors.lastname: Gilham
authors.forename: Phillip K
authors.initials: PK
authors.lastname: Darcy
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D002469:Cell Separation; D002470:Cell Survival; D016207:Cytokines / Q000378:metabolism; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D016219:Immunotherapy, Adoptive; D051379:Mice; D011946:Receptors, Antigen / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D012190:Retroviridae / Q000235:genetics; D013154:Spleen / Q000166:cytology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology / Q000378:metabolism*; D014161:Transduction, Genetic
chemical_list: D016207:Cytokines; D011946:Receptors, Antigen; D011993:Recombinant Fusion Proteins