title: An influenza A virus hemagglutinin (HA) epitope inserted in and expressed from several loci of the infectious bursal disease virus genome induces HA-specific antibodies.
abstract: The N-terminus of infectious bursal disease virus (IBDV) VP5 has been shown to be capable of tolerating the insertion of small epitopes. The objective of the present study was to determine if IBDV genomic sites, including the 5' end of vp5, could carry an influenza A virus hemagglutinin (HA) epitope. HA-expressing IBDVs were generated when the HA epitope was fused to the N-terminus of VP5 (HA5-IBDV) or VP4 (HA4-IBDV) or the C-terminus of VP1 (1HA-IBDV). Viral titers obtained after co-transfection with cDNA from the ha-containing segment and the complementary genomic segment were 1.3 × 10(4), 3.7 × 10(3) and 3.8 × 10(4) pfu/ml for HA5-IBDV, HA4-IBDV and 1HA-IBDV, respectively. The HA tag expression remained stable after 10 passages when the tag gene was inserted into the vp4 and vp1 genes. HA-IBDVs did not cause pathogenicity in specific-pathogen-free (SPF) chickens. However, only HA4-IBDV and 1HA-IBDV induced HA-specific antibodies, which were measured by ELISA with a maximum optical density (OD) value of 0.701 and 0.769, respectively, at 24 days after infection. Thus, IBDV can potentially be employed as a bivalent viral vector when the epitope is fused with VP4 or VP1.
authors.affiliation: Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN, 47907, USA.
authors.forename: Yung-Yi C
authors.initials: YY
authors.lastname: Mosley
authors.forename: Ching Ching
authors.initials: CC
authors.lastname: Wu
authors.forename: Tsang Long
authors.initials: TL
authors.lastname: Lin
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D018175:Birnaviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D002642:Chick Embryo; D002645:Chickens; D005822:Genetic Vectors / Q000235:genetics / Q000276:immunology; D016679:Genome, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007243:Infectious bursal disease virus / Q000235:genetics* / Q000276:immunology; D053122:Influenza A Virus, H3N2 Subtype / Q000235:genetics / Q000276:immunology*; D011201:Poultry Diseases / Q000276:immunology* / Q000821:virology; D013045:Species Specificity; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000276:immunology; D015678:Viral Structural Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; C107669:VP1 protein, infectious bursal disease virus; C478245:VP4 protein, infectious bursal disease virus; C518635:VP5 protein, infectious bursal disease virus; D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins; C483468:hemagglutinin, human influenza A virus