title: Complete replication of hepatitis B virus and hepatitis C virus in a newly developed hepatoma cell line.
abstract: The absence of a robust cell culture system for hepatitis B virus (HBV) and hepatitis C virus (HCV) infection has limited the analysis of the virus lifecycle and drug discovery. We have established a hepatoma cell line, HLCZ01, the first cell line, to the authors' knowledge, supporting the entire lifecycle of both HBV and HCV. HBV surface antigen (HBsAg)-positive particles can be observed in the supernatant and the lumen of the endoplasmic reticulum of the cells via electron microscopy. Interestingly, HBV and HCV clinical isolates propagate in HLCZ01 cells. Both viruses replicate in the cells without evidence of overt interference. HBV and HCV entry are blocked by antibodies against HBsAg and human CD81, respectively, and the replication of HBV and HCV is inhibited by antivirals. HLCZ01 cells mount an innate immune response to virus infection. The cell line provides a powerful tool for exploring the mechanisms of virus entry and replication and the interaction between host and virus, facilitating the development of novel antiviral agents and vaccines.
authors.affiliation: Department of Molecular Medicine, College of Biology, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, China.
authors.forename: Darong
authors.initials: D
authors.lastname: Yang
authors.forename: Chaohui
authors.initials: C
authors.lastname: Zuo
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Wang
authors.forename: Xianghe
authors.initials: X
authors.lastname: Meng
authors.forename: Binbin
authors.initials: B
authors.lastname: Xue
authors.forename: Nianli
authors.initials: N
authors.lastname: Liu
authors.forename: Rong
authors.initials: R
authors.lastname: Yu
authors.forename: Yuwen
authors.initials: Y
authors.lastname: Qin
authors.forename: Yimin
authors.initials: Y
authors.lastname: Gao
authors.forename: Qiuping
authors.initials: Q
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Zebin
authors.initials: Z
authors.lastname: Zhou
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.forename: Deming
authors.initials: D
authors.lastname: Tan
authors.forename: Yang
authors.initials: Y
authors.lastname: Guan
authors.forename: Haizhen
authors.initials: H
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology / Q000627:therapeutic use; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000473:pathology / Q000821:virology*; D045744:Cell Line, Tumor; D060085:Coinfection / Q000188:drug therapy / Q000473:pathology / Q000821:virology; D016174:Hepacivirus / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D006509:Hepatitis B / Q000188:drug therapy / Q000821:virology; D006515:Hepatitis B virus / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D006526:Hepatitis C / Q000188:drug therapy / Q000821:virology; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy / Q000473:pathology / Q000821:virology*; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D014779:Virus Replication / Q000187:drug effects / Q000502:physiology*
chemical_list: D000998:Antiviral Agents
keywords: ISGs; cccDNA; cell culture model; interferon; primary human hepatocytes