title: Overgrowth of the indigenous gut microbiome and irritable bowel syndrome.
abstract: Culture-independent molecular techniques have demonstrated that the majority of the gut microbiota is uncultivable. Application of these molecular techniques to more accurately identify the indigenous gut microbiome has moved with great pace over recent years, leading to a substantial increase in understanding of gut microbial communities in both health and a number of disorders, including irritable bowel syndrome (IBS). Use of culture-independent molecular techniques already employed to characterise faecal and, to a lesser extent, colonic mucosal microbial populations in IBS, without reliance on insensitive, traditional microbiological culture techniques, has the potential to more accurately determine microbial composition in the small intestine of patients with this disorder, at least that occurring proximally and within reach of sampling. Current data concerning culture-based and culture-independent analyses of the small intestinal microbiome in IBS are considered here.
authors.affiliation: William Bye, Naveed Ishaq, Terry D Bolin, Vic M Duncombe, Stephen M Riordan, Gastrointestinal and Liver Unit, Prince of Wales Hospital, Randwick 2031, New South Wales, Australia.
authors.forename: William
authors.initials: W
authors.lastname: Bye
authors.affiliation: William Bye, Naveed Ishaq, Terry D Bolin, Vic M Duncombe, Stephen M Riordan, Gastrointestinal and Liver Unit, Prince of Wales Hospital, Randwick 2031, New South Wales, Australia.
authors.forename: Naveed
authors.initials: N
authors.lastname: Ishaq
authors.affiliation: William Bye, Naveed Ishaq, Terry D Bolin, Vic M Duncombe, Stephen M Riordan, Gastrointestinal and Liver Unit, Prince of Wales Hospital, Randwick 2031, New South Wales, Australia.
authors.forename: Terry D
authors.initials: TD
authors.lastname: Bolin
authors.affiliation: William Bye, Naveed Ishaq, Terry D Bolin, Vic M Duncombe, Stephen M Riordan, Gastrointestinal and Liver Unit, Prince of Wales Hospital, Randwick 2031, New South Wales, Australia.
authors.forename: Vic M
authors.initials: VM
authors.lastname: Duncombe
authors.affiliation: William Bye, Naveed Ishaq, Terry D Bolin, Vic M Duncombe, Stephen M Riordan, Gastrointestinal and Liver Unit, Prince of Wales Hospital, Randwick 2031, New South Wales, Australia.
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Riordan
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D001419:Bacteria / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D001431:Bacteriological Techniques; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology; D007421:Intestine, Small / Q000187:drug effects / Q000382:microbiology*; D043183:Irritable Bowel Syndrome / Q000188:drug therapy / Q000382:microbiology*; D064307:Microbiota / Q000187:drug effects; D016896:Treatment Outcome
chemical_list: D000900:Anti-Bacterial Agents
keywords: Gut microbiome; Irritable bowel syndrome; Small intestinal bacterial overgrowth