title: Human dopamine receptor nanovesicles for gate-potential modulators in high-performance field-effect transistor biosensors.
abstract: The development of molecular detection that allows rapid responses with high sensitivity and selectivity remains challenging. Herein, we demonstrate the strategy of novel bio-nanotechnology to successfully fabricate high-performance dopamine (DA) biosensor using DA Receptor-containing uniform-particle-shaped Nanovesicles-immobilized Carboxylated poly(3,4-ethylenedioxythiophene) (CPEDOT) NTs (DRNCNs). DA molecules are commonly associated with serious diseases, such as Parkinson's and Alzheimer's diseases. For the first time, nanovesicles containing a human DA receptor D1 (hDRD1) were successfully constructed from HEK-293 cells, stably expressing hDRD1. The nanovesicles containing hDRD1 as gate-potential modulator on the conducting polymer (CP) nanomaterial transistors provided high-performance responses to DA molecule owing to their uniform, monodispersive morphologies and outstanding discrimination ability. Specifically, the DRNCNs were integrated into a liquid-ion gated field-effect transistor (FET) system via immobilization and attachment processes, leading to high sensitivity and excellent selectivity toward DA in liquid state. Unprecedentedly, the minimum detectable level (MDL) from the field-induced DA responses was as low as 10 pM in real- time, which is 10 times more sensitive than that of previously reported CP based-DA biosensors. Moreover, the FET-type DRNCN biosensor had a rapid response time (<1 s) and showed excellent selectivity in human serum.
authors.affiliation: 1] World Class University program of Chemical Convergence for Energy & Environment, School of Chemical and Biological Engineering, Seoul National University, 151-742, Korea [2].
authors.forename: Seon Joo
authors.initials: SJ
authors.lastname: Park
authors.affiliation: 1] School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Korea [2] Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge, Massachusetts 02139, USA [3].
authors.forename: Hyun Seok
authors.initials: HS
authors.lastname: Song
authors.affiliation: 1] World Class University program of Chemical Convergence for Energy & Environment, School of Chemical and Biological Engineering, Seoul National University, 151-742, Korea [2] Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
authors.forename: Oh Seok
authors.initials: OS
authors.lastname: Kwon
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Korea.
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Chung
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Korea.
authors.forename: Seung Hwan
authors.initials: SH
authors.lastname: Lee
authors.affiliation: World Class University program of Chemical Convergence for Energy & Environment, School of Chemical and Biological Engineering, Seoul National University, 151-742, Korea.
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: An
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Korea.
authors.forename: Sae Ryun
authors.initials: SR
authors.lastname: Ahn
authors.affiliation: Department of Polymer Engineering, Chonnam National University, Gwangju 500-757, Korea.
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Lee
authors.affiliation: Department of Polymer Engineering, Chonnam National University, Gwangju 500-757, Korea.
authors.forename: Hyeonseok
authors.initials: H
authors.lastname: Yoon
authors.affiliation: 1] School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Korea [2] Advanced Institutes of Convergence Technology, Suwon 443-270, Korea.
authors.forename: Tai Hyun
authors.initials: TH
authors.lastname: Park
authors.affiliation: World Class University program of Chemical Convergence for Energy & Environment, School of Chemical and Biological Engineering, Seoul National University, 151-742, Korea.
authors.forename: Jyongsik
authors.initials: J
authors.lastname: Jang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry*; D004298:Dopamine / Q000097:blood*; D004867:Equipment Design; D015870:Gene Expression; D057809:HEK293 Cells; D006801:Humans; D057230:Limit of Detection; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D011108:Polymers / Q000737:chemistry*; D017447:Receptors, Dopamine D1 / Q000737:chemistry* / Q000235:genetics; D013997:Time Factors; D014173:Transistors, Electronic
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; C583260:DRD1 protein, human; D011108:Polymers; D017447:Receptors, Dopamine D1; C121383:poly(3,4-ethylene dioxythiophene); D004298:Dopamine