title: Expression of cyclinD1 and Ki-67 proteins in gliomas and its clinical significance.
abstract: OBJECTIVE
  To investigate the expression of cyclinD1 and Ki-67 proteins in gliomas and its significance.
  
  
  METHODS
  The immunohistochemistry was used to detect the expression of cyclinD1 and Ki-67 proteins in 18 cases of normal brain tissues, 32 cases of low-grade gliomas, and 24 cases of high-grade gliomas.
  
  
  RESULTS
  The cyclinD1 positive ratio in normal brain tissues, low-grade gliomas, and high-grade gliomas were 4/18, 15/32, and 18/24, respectively, with statistically significant difference (p < 0.05). Differences were significant by pairwise comparison between normal brain tissue with high-grade gliomas and low-grade gliomas with high-grade glioma groups (p < 0.01). However, there was no significant differences between normal brain tissue with low-grade gliomas. The Ki-67 positive ratio in normal brain tissues, low-grade gliomas, and high-grade gliomas were 5/18, 21/32, and 20/24, respectively. The difference among three tissues was statistically significant (p < 0.05). Differences were significant by pairwise comparison between normal brain tissue with low-grade gliomas and normal brain tissue with high-grade glioma group (p < 0.01). There is no difference between low-grade gliomas and high-grade gliomas (p > 0.05). Spearman's rank correlation confirmed that cyclinD1 and Ki-67 was positively correlated in low-grade gliomas and high-level brain tumor (p < 0.05), but no correlation in the normal brain tissue (p > 0.05).
  
  
  CONCLUSIONS
  The expression of CyclinD1 and Ki-67 increased in gliomas, suggesting that both may play an important role in the occurrence of gliomas.
authors.affiliation: Department of Neurobiology and Anatomy, Xuzhou Medical College, Xuzhou, China. xhs51@126.com.
authors.forename: D-W
authors.initials: DW
authors.lastname: Qu
authors.forename: H-S
authors.initials: HS
authors.lastname: Xu
authors.forename: X-J
authors.initials: XJ
authors.lastname: Han
authors.forename: Y-L
authors.initials: YL
authors.lastname: Wang
authors.forename: C-J
authors.initials: CJ
authors.lastname: Ouyang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000737:chemistry* / Q000473:pathology; D016009:Chi-Square Distribution; D002648:Child; D019938:Cyclin D1 / Q000032:analysis*; D005260:Female; D005910:Glioma / Q000737:chemistry* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D011237:Predictive Value of Tests; D015854:Up-Regulation; D055815:Young Adult
chemical_list: C530104:CCND1 protein, human; D019394:Ki-67 Antigen; D019938:Cyclin D1