title: A novel P450-based biocatalyst for the selective production of chiral 2-alkanols.
abstract: A P450 monooxygenase from Nocardia farcinica (CYP154A8) catalyses the stereo- and regioselective hydroxylation of n-alkanes, still a challenging task in chemical catalysis. In a biphasic reaction system, the regioselectivity for the C2-position of C7-C9 alkanes was over 90%. The enzyme showed strict S-selectivity for all tested substrates, with enantiomeric excess (ee) of up to 91%.
authors.affiliation: Institute of Biochemistry, Heinrich-Heine University Düsseldorf, Universitätsstraße 1, 40225 Düsseldorf, Germany. Vlada.Urlacher@uni-duesseldorf.de.
authors.forename: Clemens J
authors.initials: CJ
authors.lastname: von Bühler
authors.forename: Vlada B
authors.initials: VB
authors.lastname: Urlacher
mesh_terms: D000473:Alkanes / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D002384:Catalysis; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry*; D006900:Hydroxylation; D009615:Nocardia
chemical_list: D000473:Alkanes; D001426:Bacterial Proteins; D003577:Cytochrome P-450 Enzyme System