title: Fighting in the home cage: Agonistic encounters and effects on neurobiological markers within the social decision-making network of house mice (Mus musculus).
abstract: Inbred strains of mice, such as C57Bl/6, have become preferred animal models for neurobehavioral studies. A main goal in creating inbred lines is to reduce the effects of individual genetic variation on observed phenotypes. Most studies use only males, and there is increasing evidence that agonistic interactions within the home cage may produce systematic variability in behavior and brain function. Previous studies have demonstrated that the outcomes of aggressive interactions have powerful effects on the brain and behavior, but less is known about whether aggressive interactions within the home cage have similar effects. We assessed group-housed laboratory mice C57Bl/6 for competitive ability and then tested the extent high competitive ability (CA) or low CA was related to gene and protein expression within related pathways. We focused on a broad social behavior network, including the nucleus accumbens (NAc) and bed nucleus of the stria terminalis (BNST). High CA mice had significantly more corticotropin releasing hormone receptor 2 (CRHR2) and estrogen receptor alpha (ESR1) mRNA in the BNST. Our data suggest a simple test of CA could yield valuable information that could be used to reduce error variance and increase power in neurobiological studies using mice.
authors.affiliation: Neuroscience Graduate Group, University of California, Davis, CA 95616, USA; Center for Neuroscience, University of California, Davis, CA 95616, USA; Department of Psychology, University of California, Davis, CA 95616, USA. Electronic address: ggreenberg@ucdavis.edu.
authors.forename: Gian D
authors.initials: GD
authors.lastname: Greenberg
authors.affiliation: Department of Animal Science, University of California, Davis, CA 95616, USA.
authors.forename: Chris L
authors.initials: CL
authors.lastname: Howerton
authors.affiliation: Neuroscience Graduate Group, University of California, Davis, CA 95616, USA; Center for Neuroscience, University of California, Davis, CA 95616, USA; Department of Psychology, University of California, Davis, CA 95616, USA.
authors.forename: Brian C
authors.initials: BC
authors.lastname: Trainor
mesh_terms: D000374:Aggression; D000818:Animals; D004291:Dominance-Subordination; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D009714:Nucleus Accumbens / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism; D018019:Receptors, Corticotropin-Releasing Hormone / Q000235:genetics / Q000378:metabolism; D012686:Septal Nuclei / Q000502:physiology*
chemical_list: C101531:CRF receptor type 2; D047628:Estrogen Receptor alpha; D012333:RNA, Messenger; D011944:Receptors, Androgen; D018019:Receptors, Corticotropin-Releasing Hormone
keywords: Aggression; Androgen receptor; Bed nucleus of stria terminalis; Estrogen receptor; Nucleus accumbens; Tube test