title: The effect of delivering the chemokine SDF-1α in a matrix-bound manner on myogenesis.
abstract: Several chemokines are important in muscle myogenesis and in the recruitment of muscle precursors during muscle regeneration. Among these, the SDF-1α chemokine (CXCL12) is a potent chemoattractant known to be involved in muscle repair. SDF-1α was loaded in polyelectrolyte multilayer films made of poly(L-lysine) and hyaluronan to be delivered locally to myoblast cells in a matrix-bound manner. The adsorbed amounts of SDF-1α were tuned over a large range from 100 ng/cm(2) to 5 μg/cm(2), depending on the initial concentration of SDF-1α in solution, its pH, and on the film crosslinking extent. Matrix-bound SDF-1α induced a striking increase in myoblast spreading, which was revealed when it was delivered from weakly crosslinked films. It also significantly enhanced cell migration in a dose-dependent manner, which again depended on its presentation by the biopolymeric film. The low-crosslinked film was the most efficient in boosting cell migration. Furthermore, matrix-bound SDF-1α also increased the expression of myogenic markers but the fusion index decreased in a dose-dependent manner with the adsorbed amount of SDF-1α. At high adsorbed amounts of SDF-1α, a large number of Troponin T-positive cells had only one nucleus. Overall, this work reveals the importance of the presentation mode of SDF-1α to emphasize its effect on myogenic processes. These films may be further used to provide insight into the role of SDF-1α presented by a biomaterial in physiological or pathological processes.
authors.affiliation: CNRS UMR 5628 (LMGP), 3 parvis Louis Néel, 38016 Grenoble, France.|Université Grenoble Alpes, LMGP, 3 parvis Louis Néel, 38016 Grenoble, France.
authors.forename: Fabien
authors.initials: F
authors.lastname: Dalonneau
authors.affiliation: CNRS UMR 5628 (LMGP), 3 parvis Louis Néel, 38016 Grenoble, France.|Université Grenoble Alpes, LMGP, 3 parvis Louis Néel, 38016 Grenoble, France.|FONDATION ARC, 9 rue Guy Môquet 94803 Villejuif, France.
authors.forename: Xi Qiu
authors.initials: XQ
authors.lastname: Liu
authors.affiliation: Univ. Grenoble Alpes, Institut de Biologie Structurale (IBS), F-38027 Grenoble, France.|CNRS, IBS, F-38027 Grenoble, France.|CEA, DSV, IBS, F-38027 Grenoble, France.
authors.forename: Rabia
authors.initials: R
authors.lastname: Sadir
authors.affiliation: CNRS UMR 5628 (LMGP), 3 parvis Louis Néel, 38016 Grenoble, France.|Université Grenoble Alpes, LMGP, 3 parvis Louis Néel, 38016 Grenoble, France.
authors.forename: Jorge
authors.initials: J
authors.lastname: Almodovar
authors.affiliation: Centre de Recherche en Cancérologie de Lyon, UMR INSERM 1052 - CNRS 5286, 28, rue Laennec, 69373 LYON cedex 08, France.
authors.forename: Hichem C
authors.initials: HC
authors.lastname: Mertani
authors.affiliation: CNRS UMR 5628 (LMGP), 3 parvis Louis Néel, 38016 Grenoble, France.|Université Grenoble Alpes, LMGP, 3 parvis Louis Néel, 38016 Grenoble, France.
authors.forename: Franz
authors.initials: F
authors.lastname: Bruckert
authors.affiliation: Inserm U823, ERL CNRS5284, Université Joseph Fourier, Institut Albert Bonniot, Site Santé, BP170, 38042 Grenoble cedex 9, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Albiges-Rizo
authors.affiliation: CNRS UMR 5628 (LMGP), 3 parvis Louis Néel, 38016 Grenoble, France.|Université Grenoble Alpes, LMGP, 3 parvis Louis Néel, 38016 Grenoble, France.
authors.forename: Marianne
authors.initials: M
authors.lastname: Weidenhaupt
authors.affiliation: Univ. Grenoble Alpes, Institut de Biologie Structurale (IBS), F-38027 Grenoble, France.|CNRS, IBS, F-38027 Grenoble, France.|CEA, DSV, IBS, F-38027 Grenoble, France.
authors.forename: Hugues
authors.initials: H
authors.lastname: Lortat-Jacob
authors.affiliation: CNRS UMR 5628 (LMGP), 3 parvis Louis Néel, 38016 Grenoble, France.|Université Grenoble Alpes, LMGP, 3 parvis Louis Néel, 38016 Grenoble, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Picart
mesh_terms: D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D054377:Chemokine CXCL12 / Q000008:administration & dosage* / Q000494:pharmacology; D016503:Drug Delivery Systems; D006820:Hyaluronic Acid / Q000737:chemistry*; D051379:Mice; D024510:Muscle Development / Q000187:drug effects*; D032446:Myoblasts / Q000166:cytology* / Q000187:drug effects; D011107:Polylysine / Q000737:chemistry*
chemical_list: D054377:Chemokine CXCL12; D011107:Polylysine; D006820:Hyaluronic Acid
keywords: CXCR4; Drug delivery; Migration; Myoblast; Polyelectrolyte multilayer film; SDF-1α/CXCL12