title: Inhibition of heme uptake in Pseudomonas aeruginosa by its hemophore (HasA(p)) bound to synthetic metal complexes.
abstract: The heme acquisition system A protein secreted by Pseudomonas aeruginosa (HasA(p)) can capture several synthetic metal complexes other than heme. The crystal structures of HasA(p) harboring synthetic metal complexes revealed only small perturbation of the overall HasA(p) structure. An inhibitory effect upon heme acquisition by HasA(p) bearing synthetic metal complexes was examined by monitoring the growth of Pseudomonas aeruginosa PAO1. HasA(p) bound to iron-phthalocyanine inhibits heme acquisition in the presence of heme-bound HasA(p) as an iron source.
authors.affiliation: Department of Chemistry, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8602 (Japan).
authors.forename: Chikako
authors.initials: C
authors.lastname: Shirataki
authors.forename: Osami
authors.initials: O
authors.lastname: Shoji
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Terada
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Ozaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sugimoto
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Shiro
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Watanabe
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism*; D056831:Coordination Complexes / Q000737:chemistry / Q000378:metabolism*; D018360:Crystallography, X-Ray; D006418:Heme / Q000737:chemistry / Q000378:metabolism*; D007211:Indoles / Q000737:chemistry / Q000378:metabolism; D007501:Iron / Q000737:chemistry* / Q000378:metabolism; D054833:Isoindoles; D008652:Mesoporphyrins / Q000737:chemistry / Q000378:metabolism; D056004:Molecular Dynamics Simulation; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D056831:Coordination Complexes; C114820:HasAp protein, Pseudomonas aeruginosa; D007211:Indoles; D054833:Isoindoles; D008652:Mesoporphyrins; D006418:Heme; C031468:mesoporphyrin IX; D007501:Iron; C013647:phthalocyanine
keywords: HasA; growth inhibition; heme proteins; iron complexes; protein structures