title: Formation of a stacked dimeric G-quadruplex containing bulges by the 5'-terminal region of human telomerase RNA (hTERC).
abstract: We investigate the structure formed by the first 18-nt of the 5'-terminal region of the human telomerase RNA (hTERC or hTR) using gel electrophoresis and UV, CD, and NMR spectroscopy. Our data suggest that this 18-nt sequence, r(GGGUUGCGGAGGGUGGGC), can form a stacked dimeric G-quadruplex in potassium solution. The two subunits, each being a three-layer parallel-stranded G-quadruplex with a cytosine bulge, are stacked at their 5'-end. The formation of this stacked dimeric G-quadruplex containing bulges could be biologically relevant for the dimerization and other interactions of the active human telomerase.
authors.affiliation: School of Physical and Mathematical Sciences, Nanyang Technological University , Singapore 637371.
authors.forename: Herry
authors.initials: H
authors.lastname: Martadinata
authors.forename: Anh Tuân
authors.initials: AT
authors.lastname: Phan
mesh_terms: D001483:Base Sequence; D019281:Dimerization; D054856:G-Quadruplexes; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry* / Q000235:genetics; D019098:Telomerase / Q000737:chemistry* / Q000235:genetics
chemical_list: C422952:telomerase RNA; D012313:RNA; D019098:Telomerase