title: Trichomonas spp. in pigeons: detection by OSOM Trichomonas Rapid Test.
abstract: The efficacy of the OSOM Trichomonas Rapid Test (developed for rapid diagnosis of human Trichomonas vaginalis) in detection of Trichomonas spp. in pigeons (Columba livia) was investigated. Two oral cavity swabs were taken from 50 farm pigeons. Cultivation in Diamond Trichomonas medium was used as a reference method. According to a morphological determination, Trichomonas gallinae was the only protozoan found; however, no further molecular analysis was conducted. The OSOM Trichomonas test was positive in 39 oral swabs. In comparison with the cultivation method three samples were identified as false negative and one as false positive. Test specificity and sensitivity were established as 93% and 90%, respectively. Using Cohen's Kappa, the concordance between the two testing methods was found to be strong (kappa = 0.7506, 95% CI = 0.5162-0.9850). The OSOM Trichomonas test is not able to distinguish between Trichomonas species; however, results suggest that the test is suitable for the rapid detection of Trichomonas spp. infection in pigeons.
authors.affiliation: Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, 165 21 Prague 6-Suchdol, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Valek
authors.affiliation: Department of Ecology, Faculty of Environmental Sciences, Czech University of Life Sciences, 165 21 Prague 6-Suchdol, Czech Republic.
authors.forename: Tomas
authors.initials: T
authors.lastname: Kunca
authors.affiliation: Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, 165 21 Prague 6-Suchdol, Czech Republic.
authors.forename: Iva
authors.initials: I
authors.lastname: Langrova
authors.affiliation: Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, 165 21 Prague 6-Suchdol, Czech Republic.
authors.forename: Helena
authors.initials: H
authors.lastname: Hartlova
authors.affiliation: Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, 165 21 Prague 6-Suchdol, Czech Republic.
authors.forename: Adela
authors.initials: A
authors.lastname: Brozova
authors.affiliation: Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, 165 21 Prague 6-Suchdol, Czech Republic.
authors.forename: Ivana
authors.initials: I
authors.lastname: Jankovska
authors.affiliation: Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, 165 21 Prague 6-Suchdol, Czech Republic.
authors.forename: Marie
authors.initials: M
authors.lastname: Kudrnacova
authors.affiliation: Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, 165 21 Prague 6-Suchdol, Czech Republic.
authors.forename: Vladislav
authors.initials: V
authors.lastname: Sloup
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000175:diagnosis* / Q000469:parasitology; D002846:Chromatography, Affinity / Q000379:methods* / Q000662:veterinary; D010856:Columbidae; D018153:Czech Republic; D014244:Trichomonas / Q000302:isolation & purification*; D014245:Trichomonas Infections / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*