title: Associations of cytological endometritis with energy metabolism and inflammation during the periparturient period and early lactation in dairy cows.
abstract: Multiparous Holstein cows (n=108) were used to determine the associations of cytological endometritis (CE) with plasma nonesterified fatty acids (NEFA) and β-hydroxybutyrate (BHBA) as markers of energy metabolism, calculated energy balance (EB), and plasma haptoglobin (Hp) as a marker of inflammation during the periparturient period and early lactation. Evaluation of endometrial cytology by low-volume uterine lavage was conducted on 1d between 40 and 60 d postcalving. The incidence of CE among cows sampled was 40%. The area under the curve (AUC) was calculated for both NEFA and BHBA using data collected from 3 wk before to 3 wk after parturition. Data for NEFA and BHBA AUC were stratified into prepartum (wk -3 to parturition) and postpartum (parturition to wk +3) for statistical analysis. Prepartum AUC for neither NEFA nor BHBA was associated with subsequent CE; however, cows that subsequently developed CE tended to have higher postpartum AUC for NEFA and had higher postpartum AUC for BHBA. Consistent with the results for plasma NEFA and BHBA, calculated EB during the prepartum period was not different in cows that did or did not develop CE; however, cows with CE had lower EB during the 6-wk postpartum period compared with cows without CE. Analysis of EB by week (wk -3 to -1 before calving and wk +1 to +6 postcalving) indicated that EB in cows with CE was lower at wk +1, +2, and +3 and tended to be lower at wk +6 than cows without CE. Plasma Hp concentrations were analyzed from wk +1 to +8 of lactation; concentrations of Hp were not different during either wk +1 or the entire postpartum period between cows that did or did not develop CE. These results suggest that lower energy status during the first 3 wk postpartum, but not necessarily systemic inflammation, is associated with subsequent development of CE.
authors.affiliation: Department of Animal Science, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853.
authors.forename: T
authors.initials: T
authors.lastname: Yasui
authors.affiliation: Department of Animal Science, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853.
authors.forename: K
authors.initials: K
authors.lastname: McCann
authors.affiliation: Department of Clinical Sciences, and College of Veterinary Medicine, Cornell University, Ithaca, NY 14853.
authors.forename: R O
authors.initials: RO
authors.lastname: Gilbert
authors.affiliation: Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853.
authors.forename: D V
authors.initials: DV
authors.lastname: Nydam
authors.affiliation: Department of Animal Science, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853. Electronic address: tro2@cornell.edu.
authors.forename: T R
authors.initials: TR
authors.lastname: Overton
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000097:blood; D000818:Animals; D002417:Cattle / Q000378:metabolism*; D002418:Cattle Diseases / Q000503:physiopathology*; D004716:Endometritis / Q000503:physiopathology / Q000662:veterinary*; D004734:Energy Metabolism; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D006242:Haptoglobins / Q000032:analysis; D007249:Inflammation / Q000662:veterinary; D007774:Lactation; D036801:Parturition; D058725:Peripartum Period; D049590:Postpartum Period
chemical_list: D005230:Fatty Acids, Nonesterified; D006242:Haptoglobins; D020155:3-Hydroxybutyric Acid
keywords: endometritis; energy status; haptoglobin