title: Biosynthesis, structural architecture and biotechnological potential of bacterial tannase: a molecular advancement.
abstract: Tannin-rich materials are abundantly generated as wastes from several agroindustrial activities. Therefore, tannase is an interesting hydrolase, for bioconversion of tannin-rich materials into value added products by catalyzing the hydrolysis of ester and depside bonds and unlocked a new prospect in different industrial sectors like food, beverages, pharmaceuticals, etc. Microorganisms, particularly bacteria are one of the major sources of tannase. In the last decade, cloning and heterologous expression of novel tannase genes and structural study has gained momentum. In this article, we have emphasized critically on bacterial tannase that have gained worldwide research interest for their diverse properties. The present paper delineate the developments that have taken place in understanding the role of tannase action, microbial sources, various cultivation aspects, downstream processing, salient biochemical properties, structure and active sites, immobilization, efforts in cloning and overexpression and with special emphasis on recent molecular and biotechnological achievements.
authors.affiliation: Department of Microbiology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Arijit
authors.initials: A
authors.lastname: Jana
authors.affiliation: Department of Microbiology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Suman Kumar
authors.initials: SK
authors.lastname: Halder
authors.affiliation: Department of Microbiology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Amrita
authors.initials: A
authors.lastname: Banerjee
authors.affiliation: Department of Microbiology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Tanmay
authors.initials: T
authors.lastname: Paul
authors.affiliation: Department of Microbiology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Bikash Ranjan
authors.initials: BR
authors.lastname: Pati
authors.affiliation: Department of Microbiology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Keshab Chandra
authors.initials: KC
authors.lastname: Mondal
authors.affiliation: Department of Microbiology, Vidyasagar University, Midnapore 721102, West Bengal, India. Electronic address: pkdmvu@gmail.com.
authors.forename: Pradeep Kumar
authors.initials: PK
authors.lastname: Das Mohapatra
mesh_terms: D001419:Bacteria / Q000201:enzymology*; D001709:Biotechnology / Q000379:methods*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D005285:Fermentation; D013634:Tannins / Q000737:chemistry / Q000378:metabolism
chemical_list: D013634:Tannins; D002265:Carboxylic Ester Hydrolases; C022711:tannase
keywords: Bacterial tannase; Biochemical property; Cloning; Immobilization; Structural characterization