title: Virtual fragment screening on GPCRs: a case study on dopamine D3 and histamine H4 receptors.
abstract: Prospective structure based virtual fragment screening methodologies on two GPCR targets namely the dopamine D3 and the histamine H4 receptors with a library of 12,905 fragments were evaluated. Fragments were docked to the X-ray structure and the homology model of the D3 and H4 receptors, respectively. Representative receptor conformations for ensemble docking were obtained from molecular dynamics trajectories. In vitro confirmed hit rates ranged from 16% to 32%. Hits had high ligand efficiency (LE) values in the range of 0.31-0.74 and also acceptable lipophilic efficiency. The X-ray structure, the homology model and structural ensembles were all found suitable for docking based virtual screening of fragments against these GPCRs. However, there was little overlap among different hit sets and methodologies were thus complementary to each other.
authors.affiliation: Gedeon Richter Plc, H-1475, P.O.B. 27, Budapest, Hungary.
authors.forename: Márton
authors.initials: M
authors.lastname: Vass
authors.affiliation: Gedeon Richter Plc, H-1475, P.O.B. 27, Budapest, Hungary.
authors.forename: Éva
authors.initials: É
authors.lastname: Schmidt
authors.affiliation: Gedeon Richter Plc, H-1475, P.O.B. 27, Budapest, Hungary.
authors.forename: Ferenc
authors.initials: F
authors.lastname: Horti
authors.affiliation: Research Centre for Natural Sciences of the Hungarian Academy of Sciences, H-1525, P.O.B. 17, Budapest, Hungary. Electronic address: keseru.gyorgy@ttk.mta.hu.
authors.forename: György M
authors.initials: GM
authors.lastname: Keserű
mesh_terms: D000818:Animals; D016466:CHO Cells; D002478:Cells, Cultured; D003412:Cricetulus; D018360:Crystallography, X-Ray; D057166:High-Throughput Screening Assays; D006801:Humans; D008958:Models, Molecular; D011487:Protein Conformation; D050637:Receptors, Dopamine D3 / Q000737:chemistry*; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry*; D011968:Receptors, Histamine / Q000737:chemistry*; D000074040:Receptors, Histamine H4
chemical_list: C418607:HRH4 protein, human; D050637:Receptors, Dopamine D3; D043562:Receptors, G-Protein-Coupled; D011968:Receptors, Histamine; D000074040:Receptors, Histamine H4
keywords: Dopamine D3 receptor; Ensemble docking; Fragment docking; Fragment screening; G protein-coupled receptors; Histamine H4 receptor; Virtual screening