title: Changes in mitochondrial functioning with electromagnetic radiation of ultra high frequency as revealed by electron paramagnetic resonance methods.
abstract: OBJECTIVE
  To study the effects of electromagnetic radiation (EMR) of ultra high frequency (UHF) in the doses equivalent to the maximal permitted energy load for the staffs of the radar stations on the biochemical processes that occur in the cell organelles.
  
  
  METHODS
  Liver, cardiac and aorta tissues from the male rats exposed to non-thermal UHF EMR in pulsed and continuous modes were studied during 28 days after the irradiation by the electron paramagnetic resonance (EPR) methods including a spin trapping of superoxide radicals.
  
  
  RESULTS
  The qualitative and quantitative disturbances in electron transport chain (ETC) of mitochondria are registered. A formation of the iron-nitrosyl complexes of nitric oxide (NO) radicals with the iron-sulphide (FeS) proteins, the decreased activity of FeS-protein N2 of NADH-ubiquinone oxidoreductase complex and flavo-ubisemiquinone growth combined with the increased rates of superoxide production are obtained.
  
  
  CONCLUSIONS
  (i) Abnormalities in the mitochondrial ETC of liver and aorta cells are more pronounced for animals radiated in a pulsed mode; (ii) the alterations in the functioning of the mitochondrial ETC cause increase of superoxide radicals generation rate in all samples, formation of cellular hypoxia, and intensification of the oxide-initiated metabolic changes; and (iii) electron paramagnetic resonance methods could be used to track the qualitative and quantitative changes in the mitochondrial ETC caused by the UHF EMR.
authors.affiliation: R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology NAS of Ukraine , Kiev.
authors.forename: Anatoly
authors.initials: A
authors.lastname: Burlaka
authors.forename: Marina
authors.initials: M
authors.lastname: Selyuk
authors.forename: Marat
authors.initials: M
authors.lastname: Gafurov
authors.forename: Sergei
authors.initials: S
authors.lastname: Lukin
authors.forename: Viktoria
authors.initials: V
authors.lastname: Potaskalova
authors.forename: Evgeny
authors.initials: E
authors.lastname: Sidorik
mesh_terms: D000818:Animals; D060733:Electromagnetic Radiation; D004578:Electron Spin Resonance Spectroscopy; D008297:Male; D008928:Mitochondria / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D009928:Organ Specificity; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors