title: Crystallization and preliminary X-ray analysis of the catalytic domains of Paenibacillus sp. strain FPU-7 cell-surface-expressed chitinase ChiW.
abstract: The polysaccharide chitin is effectively hydrolyzed and utilized as a carbon and nitrogen source by the Gram-positive bacterium Paenibacillus sp. strain FPU-7. ChiW is a unique cell-surface-expressed chitinase among the Paenibacillus sp. strain FPU-7-secreted chitinases. An N-terminally truncated ChiW protein, primarily comprised of the two catalytic domains of the full-length protein, was successfully overexpressed in Escherichia coli, purified as a functional recombinant protein with a molecular mass of approximately 98 kDa and crystallized. Preliminary X-ray analysis showed that the crystal diffracted to 1.93 Å resolution and belonged to the orthorhombic space group P212121, with unit-cell parameters a = 112.1, b = 128.2, c = 162.6 Å, suggesting the presence of two molecules in an asymmetric unit.
authors.affiliation: Department of Bioscience, Fukui Prefectural University, 4-1-1 Matsuokakenjyoujima, Eiheiji-cho, Yoshida-gun, Fukui 910-1195, Japan.
authors.forename: Takafumi
authors.initials: T
authors.lastname: Itoh
authors.affiliation: Department of Bioscience, Fukui Prefectural University, 4-1-1 Matsuokakenjyoujima, Eiheiji-cho, Yoshida-gun, Fukui 910-1195, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Hibi
authors.affiliation: Department of Bioscience, Fukui Prefectural University, 4-1-1 Matsuokakenjyoujima, Eiheiji-cho, Yoshida-gun, Fukui 910-1195, Japan.
authors.forename: Ikumi
authors.initials: I
authors.lastname: Sugimoto
authors.affiliation: Department of Bioscience, Fukui Prefectural University, 4-1-1 Matsuokakenjyoujima, Eiheiji-cho, Yoshida-gun, Fukui 910-1195, Japan.
authors.forename: Fumiko
authors.initials: F
authors.lastname: Suzuki
authors.affiliation: Department of Molecular Biology and Chemistry, Faculty of Medicine, University of Fukui, 23-3 Matsuokashimoaitsuki, Eiheiji-cho, Yoshida-gun, Fukui 910-1195, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Fujii
authors.affiliation: Department of Environmental and Biotechnological Frontier Engineering, Fukui University of Technology, 3-6-1 Gakuen, Fukui, Fukui 910-1195, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Taketo
authors.affiliation: Department of Bioscience, Fukui Prefectural University, 4-1-1 Matsuokakenjyoujima, Eiheiji-cho, Yoshida-gun, Fukui 910-1195, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Kimoto
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D020134:Catalytic Domain; D002688:Chitinases / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D056507:Paenibacillus / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D002688:Chitinases
keywords: ChiW; Paenibacillus sp. strain FPU-7; cell-surface-expressed chitinase