title: Expression of genomic functional estrogen receptor 1 in mouse sertoli cells.
abstract: There is no consensus whether Sertoli cells express estrogen receptor 1 (Esr1). Reverse transcription-polymerase chain reaction, Western blot, and immunofluorescence demonstrated that mouse Sertoli cell lines, TM4, MSC-1, and 15P-1, and purified primary mouse Sertoli cells (PSCs) contained Esr1 messenger RNA and proteins. Incubation of Sertoli cells with 17β-estradiol (E2) or ESR1 agonist stimulated the expression of an estrogen responsive gene Greb1, which was prevented by ESR inhibitor or ESR1 antagonist. Overexpression of Esr1 in MSC-1 enhanced E2-induced Greb1 expression, while knockdown of Esr1 by small interfering RNA in TM4 attenuated the response. Furthermore, E2-induced Greb1 expression was abolished in the PSCs isolated from Amh-Cre/Esr1-floxed mice in which Esr1 in Sertoli cells were selectively deleted. Chromatin immunoprecipitation assays indicated that E2-induced Greb1 expression in Sertoli cells was mediated by binding of ESR1 to estrogen responsive elements. In summary, ligand-dependent nuclear ESR1 was present in mouse Sertoli cells and mediates a classical genomic action of estrogens.
authors.affiliation: Department of OB/GYN & Women's Health, University of Louisville School of Medicine, Louisville, KY, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Lin
authors.affiliation: Department of OB/GYN & Women's Health, University of Louisville School of Medicine, Louisville, KY, USA.
authors.forename: Jia
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of OB/GYN & Women's Health, University of Louisville School of Medicine, Louisville, KY, USA.
authors.forename: Xian
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of OB/GYN & Women's Health, University of Louisville School of Medicine, Louisville, KY, USA.
authors.forename: Shengqiang
authors.initials: S
authors.lastname: Li
authors.affiliation: Division of Life Sciences, Alltech, Nicholasville, KY, USA.
authors.forename: Zijian
authors.initials: Z
authors.lastname: Lan
authors.affiliation: Department of Comparative Biosciences, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
authors.forename: Jay
authors.initials: J
authors.lastname: Ko
authors.affiliation: Department of OB/GYN & Women's Health, University of Louisville School of Medicine, Louisville, KY, USA zhenmin.lei@louisville.edu.
authors.forename: Zhenmin
authors.initials: Z
authors.lastname: Lei
mesh_terms: D000818:Animals; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000494:pharmacology; D047628:Estrogen Receptor alpha / Q000819:agonists / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D047629:Estrogen Receptor beta / Q000235:genetics / Q000378:metabolism; D004967:Estrogens / Q000494:pharmacology; D005786:Gene Expression Regulation; D008297:Male; D018345:Mice, Knockout; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D012708:Sertoli Cells / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors; D014162:Transfection
chemical_list: D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens; D012333:RNA, Messenger; D004958:Estradiol
keywords: Esr1; Sertoli cells; estrogen; gene regulation; mouse; testis