title: Analysis of the small RNA spf in the plant pathogen Pseudomonas syringae pv. tomato strain DC3000.
abstract: Bacteria contain small non-coding RNAs (ncRNAs) that are typically responsible for altering transcription, translation or mRNA stability. ncRNAs are important because they often regulate virulence factors and susceptibility to various stresses. Here, the regulation of a recently described ncRNA of Pseudomonas syringae DC3000, spot 42 (now referred to as spf), was investigated. A putative RpoE binding site was identified upstream of spf in strain DC3000. RpoE is shown to regulate the expression of spf. Also, deletion of spf results in increased sensitivity to hydrogen peroxide compared with the wild-type strain, suggesting that spf plays a role in susceptibility to oxidative stress. Furthermore, expression of alg8 is shown to be influenced by spf, suggesting that this ncRNA plays a role in alginate biosynthesis. Structural and comparative genomic analyses show this ncRNA is well conserved among the pseudomonads. The findings provide new information on the regulation and role of this ncRNA in P. syringae.
authors.affiliation: Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, USA.
authors.forename: So Hae
authors.initials: SH
authors.lastname: Park
authors.affiliation: Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Zhongmeng
authors.initials: Z
authors.lastname: Bao
authors.affiliation: Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Bronwyn G
authors.initials: BG
authors.lastname: Butcher
authors.affiliation: Plant-Microbe Interactions Research Unit, Robert W. Holley Center for Agriculture and Health, Agricultural Research Service, United States Department of Agriculture, Ithaca, NY 14853, USA.|Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Katherine
authors.initials: K
authors.lastname: D'Amico
authors.affiliation: Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Yun
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Plant-Microbe Interactions Research Unit, Robert W. Holley Center for Agriculture and Health, Agricultural Research Service, United States Department of Agriculture, Ithaca, NY 14853, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Stodghill
authors.affiliation: Plant-Microbe Interactions Research Unit, Robert W. Holley Center for Agriculture and Health, Agricultural Research Service, United States Department of Agriculture, Ithaca, NY 14853, USA.|Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Schneider
authors.affiliation: Plant-Microbe Interactions Research Unit, Robert W. Holley Center for Agriculture and Health, Agricultural Research Service, United States Department of Agriculture, Ithaca, NY 14853, USA.|Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Samuel
authors.initials: S
authors.lastname: Cartinhour
authors.affiliation: Plant-Microbe Interactions Research Unit, Robert W. Holley Center for Agriculture and Health, Agricultural Research Service, United States Department of Agriculture, Ithaca, NY 14853, USA.|Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, USA.
authors.forename: M J
authors.initials: MJ
authors.lastname: Filiatrault
mesh_terms: D000464:Alginates; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D020723:Glucuronic Acid / Q000096:biosynthesis; D006603:Hexuronic Acids; D006861:Hydrogen Peroxide / Q000633:toxicity; D018384:Oxidative Stress; D010935:Plant Diseases / Q000382:microbiology; D044224:Pseudomonas syringae / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D058727:RNA, Small Untranslated / Q000096:biosynthesis* / Q000235:genetics; D012808:Sigma Factor / Q000378:metabolism
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D058727:RNA, Small Untranslated; D012808:Sigma Factor; C063202:sporulation-specific sigma factors; D020723:Glucuronic Acid; D006861:Hydrogen Peroxide