title: Spatial expression of claudin family members in various organs of mice.
abstract: Claudins (CLDNs) are tetraspan transmembrane proteins, which are components of tight junctions. The CLDN family is composed of 27 members that are responsible for paracellular transport and certain CLDNs form charge-selective ion channels. CLDNs have two extracellular loops, and the charge of the first extracellular loop determines the ion selectivity of each CLDN. Although the expression and function of each CLDN have been previously investigated, the distribution of CLDNs in various target organs remains to be determined. In the present study, the tissue-specific mRNA distribution of CLDNs (1-5, 7-8, 10a and b, 11-12, 14-17 and 19) in the duodenum, ileum, colon, kidney, liver and lung were defined. Among the tested CLDNs, CLDN1, 2, 12 and 16 were selected for further investiagtion. It was observed that CLDN1, CLDN2 and CLDN12 transcripts and proteins were particularly abundant in the investigated organs. Notably, immune-reactive CLDN16 was detected in a tissue-specific manner and shown in the renal tubules and portal vein. The tested CLDNs were localized to intercellular apical junctions in the epithelium of the intestine, renal tubule and bronchus. Based on this novel information, the presence of several types of CLDNs is of interest as CLDNs may promote or dampen the paracellular diffusion of specific ions.
authors.affiliation: Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 361‑763, Republic of Korea.
authors.forename: Inho
authors.initials: I
authors.lastname: Hwang
authors.affiliation: Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 361‑763, Republic of Korea.
authors.forename: Hyun
authors.initials: H
authors.lastname: Yang
authors.affiliation: Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 361‑763, Republic of Korea.
authors.forename: Hong-Seok
authors.initials: HS
authors.lastname: Kang
authors.affiliation: Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 361‑763, Republic of Korea.
authors.forename: Chang-Hwan
authors.initials: CH
authors.lastname: Ahn
authors.affiliation: Laboratory of Veterinary Physiology, College of Veterinary Medicine, Kangwon National University, Chuncheon, Gangwon 200‑701, Republic of Korea.
authors.forename: Geun-Shik
authors.initials: GS
authors.lastname: Lee
authors.affiliation: Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 361‑763, Republic of Korea.
authors.forename: Eui-Ju
authors.initials: EJ
authors.lastname: Hong
authors.affiliation: Department of Biomaterials Science, College of National Resources and Life Science, Pusan National University, Miryang, Pusan 627‑706, Republic of Korea.
authors.forename: Beum-Soo
authors.initials: BS
authors.lastname: An
authors.affiliation: Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 361‑763, Republic of Korea.
authors.forename: Eui-Bae
authors.initials: EB
authors.lastname: Jeung
mesh_terms: D000818:Animals; D057167:Claudins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D008297:Male; D051379:Mice; D005810:Multigene Family; D009928:Organ Specificity / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D057167:Claudins; D012333:RNA, Messenger