title: [Applicability of PCR methods for detection of shrimp and crab in processed food].
abstract: According to Japanese food allergen labeling regulations, an ELISA screening test is used for detection of crustacean proteins in food and a shrimp/crab-PCR confirmation test is used to confirm a positive ELISA screening test and to exclude false positives. Forty-six kinds of processed foods labeled as containing shrimp/crab were subjected to ELISA screening test and PCR confirmation test and the usefulness of the shrimp/crab-PCR was evaluated. Twenty-seven of the 46 samples contained total crustacean protein levels of 10 ppm or more in the ELISA screening test. All of the samples were positive in the shrimp/crab-PCR confirmation test. The results of the confirmation test were consistent with the declaration in the list of ingredients and with the results of the ELISA screening test. The shrimp/crab-PCR confirmation test was demonstrated to be applicable to various kinds of foods, including powder, extract, seasoning paste, prepared frozen food, snack food, retort food and canned food.
authors.affiliation: House Foods Group Inc.
authors.forename: Hiromu
authors.initials: H
authors.lastname: Taguchi
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Nagatomi
authors.forename: Ryo
authors.initials: R
authors.lastname: Kikuchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Hirao
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000032:analysis*; D003445:Crustacea; D004797:Enzyme-Linked Immunosorbent Assay; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D005512:Food Hypersensitivity / Q000517:prevention & control; D005515:Food Labeling / Q000331:legislation & jurisprudence; D007564:Japan; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D060829:Arthropod Proteins