title: Rabies virus glycoprotein and immune response pattern using recombinant protein or recombinant RNA viral vectors.
abstract: The present study shows the humoral and cellular aspects of immune response generated by a recombinant rabies virus glycoprotein (rRVGP) as compared to those generated by viral vector carrying the RNA coding for this protein (RVGP-RNA). The rRVGP was synthesized by stably transfected Drosophila melanogaster Schneider 2 (S2) cells and the RVGP-RNA was carried by a recombinant Semiliki Forest Virus (SFV-RVGP). The data show that protein as well as the RNA vaccine was capable of inducing reasonably acceptable levels of antibodies as compared to the optimized commercial whole virus vaccine. As expected, the RNA vaccine was clearly more effective than the protein vaccines in inducing a cellular immune response, as evaluated by the IgG2a/IgG1 ratio and synthesis of interferon gamma (IFNγ) and interleukin 2 (IL2). Our study supports the importance of vaccine designing taking into consideration the concept of DNA/RNA ability to induce an effective cell immune response.
authors.affiliation: Laboratório de Imunologia Viral, Instituto Butantan, Av. Vital Brazil 1500, 05503-900 São Paulo, Brazil.
authors.forename: Renato M
authors.initials: RM
authors.lastname: Astray
authors.affiliation: Laboratório de Imunologia Viral, Instituto Butantan, Av. Vital Brazil 1500, 05503-900 São Paulo, Brazil.
authors.forename: Daniella C
authors.initials: DC
authors.lastname: Ventini
authors.affiliation: Laboratório de Imunologia Viral, Instituto Butantan, Av. Vital Brazil 1500, 05503-900 São Paulo, Brazil.
authors.forename: Vera L L
authors.initials: VL
authors.lastname: Boldorini
authors.affiliation: Laboratório de Imunologia Viral, Instituto Butantan, Av. Vital Brazil 1500, 05503-900 São Paulo, Brazil.
authors.forename: Fernanda G
authors.initials: FG
authors.lastname: Silva
authors.affiliation: Laboratório de Imunologia Viral, Instituto Butantan, Av. Vital Brazil 1500, 05503-900 São Paulo, Brazil.
authors.forename: Mayra P
authors.initials: MP
authors.lastname: Rocca
authors.affiliation: Laboratório de Imunologia Viral, Instituto Butantan, Av. Vital Brazil 1500, 05503-900 São Paulo, Brazil. Electronic address: carlos.pereira@butantan.gov.br.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Pereira
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000276:immunology*; D002460:Cell Line; D004331:Drosophila melanogaster; D006023:Glycoproteins / Q000276:immunology*; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000276:immunology; D007376:Interleukin-2 / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012313:RNA; D011818:Rabies / Q000517:prevention & control*; D011819:Rabies Vaccines / Q000235:genetics / Q000276:immunology*; D011820:Rabies virus; D011994:Recombinant Proteins / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D006023:Glycoproteins; D007074:Immunoglobulin G; D007376:Interleukin-2; C039919:RNA, recombinant; D011819:Rabies Vaccines; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; C064951:glycoprotein G, Rabies virus; D012313:RNA; D007371:Interferon-gamma
keywords: Immune response; Protein; RNA; Rabies; Vaccine