title: New bioactive metabolites from a crown gall induced on an Eucalyptus tereticornis Sm. tree.
abstract: Applying a bioactivity-guided isolation strategy for the ethanolic extract of crown gall tumours induced on an Eucalyptus tereticornis tree, two new compounds in addition to a known one were isolated. The new compounds were identified as an amino acid derivative named 1-ethyl-6-(1'-methyl-1'-phenylethyl) piperidin-2-one (1) and a lanostane tetracyclic triterpene named 3beta-hydroxy-24-methyllanosta-8,17(20),24(28)-trien-22-oic acid (2), together with stigmasterol-3-O-glucoside (3). The three compounds exhibited significant cytotoxic activity against two human cell lines, breast (MCF7) and colon (HCT116), with IC50 values of 1.01, 1.54, and 2.15 microg/ml, respectively, against MCF7 and 3.49, 3.83, and 3.39 microg/ ml, respectively, against HCT116. Furthermore, in rats elevated levels of blood cholesterol, triglycerides, and low-density lipoprotein (LDLc) were significantly reduced, while the level of high-density lipoprotein (HDLc) was significantly increased by administration of the ethanolic extract as well as of 3. These results support a correlation between the reduction of blood cholesterol levels and improvement of colorectal cancer.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Kasr el-Aini street, Cairo 11562, Egypt.
authors.forename: Maha M
authors.initials: MM
authors.lastname: Salama
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Kasr el-Aini street, Cairo 11562, Egypt.
authors.forename: Shahira M
authors.initials: SM
authors.lastname: Ezzat
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Kasr el-Aini street, Cairo 11562, Egypt.
authors.forename: Riham Salah
authors.initials: RS
authors.lastname: El Dine
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Kasr el-Aini street, Cairo 11562, Egypt.
authors.forename: Aly M
authors.initials: AM
authors.lastname: El-Sayed
authors.affiliation: Department of Pharmacology, National Research Centre, Giza 12622, Egypt.
authors.forename: Amany A
authors.initials: AA
authors.lastname: Sleem
mesh_terms: D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D005052:Eucalyptus / Q000378:metabolism*; D006801:Humans; D007928:Lethal Dose 50; D010941:Plant Tumors