title: Starved human fibroblasts secrete acidic proteins inducing post re-feeding proliferation and in vitro cell migration: a potential tool for wound healing.
abstract: BACKGROUND
  There are several reports indicating that starved fibroblasts show higher proliferation rates when re-fed with foetal bovine serum. We have evidence demonstrating that this phenomenon is related to secretory proteins which may be beneficial to wound healing.
  
  
  RESULTS
  After re-feeding, 16 and 72 h serum-starved fibroblasts showed the highest and lowest proliferation rates, 1.59 and 0.51-fold difference compared to the non-starved control, respectively (P < 0.05). However, the latest value could be normalised by incubating cells with 16 h-starved fibroblast cell culture supernatant (16-SFS), prior to re-feeding. A strong correlation was found between total protein level in starved fibroblast culture supernatants and post re-feeding proliferation rates (r(2) = 0.90, P < 0.001). Two-dimensional gel electrophoresis analysis of 16-SFS confirmed the presence of proteins with relative molecular weights of 10-120 kDa and pI ranging from 4 to 6. A significant difference in calcium influx course was found between 16-SFS and the negative control (Dulbecco's Modified Eagle Medium) (P < 0.05). There was no significant difference in Ca(2+) concentrations after 1 h between non-starved controls and 16-SFS-treated fibroblasts. The scratch test demonstrated that the 16-SFS is able to induce fibroblast migration.
  
  
  CONCLUSIONS
  We concluded that human starved fibroblasts secrete proteins that are able to induce post re-feeding cell proliferation and fibroblasts migration, probably through the induction of a sustained calcium influx. This is worth being considered as a potential tool for wound healing.
authors.affiliation: Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran; University of Guilan, Rasht, Iran.
authors.forename: Monireh
authors.initials: M
authors.lastname: Golpour
authors.forename: Sadegh
authors.initials: S
authors.lastname: Fattahi
authors.forename: Haleh Akhavan
authors.initials: HA
authors.lastname: Niaki
authors.forename: Abbas
authors.initials: A
authors.lastname: Hadipoor
authors.forename: Zeinab
authors.initials: Z
authors.lastname: Abedian
authors.forename: Gholam Reza
authors.initials: GR
authors.lastname: Ahangarian
authors.forename: Hadi
authors.initials: H
authors.lastname: Parsian
authors.forename: Abbas
authors.initials: A
authors.lastname: Mosapour
authors.forename: Hamid Reza
authors.initials: HR
authors.lastname: Khorasani
authors.forename: Hamid Reza
authors.initials: HR
authors.lastname: Vaziri
authors.forename: Ali
authors.initials: A
authors.lastname: Bijani
authors.forename: Amrollah
authors.initials: A
authors.lastname: Mostafazadeh
mesh_terms: D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology; D006801:Humans; D007223:Infant; D008297:Male; D011506:Proteins / Q000378:metabolism; D014945:Wound Healing
chemical_list: D011506:Proteins
keywords: Fibroblast; Migration; Proliferation; Re-feeding; Wound healing