title: In vivo stimulatory effect of multi-component herbal remedy PADMA 28 on mitogen-induced proliferation of mice splenic lymphocytes and their chemokinetic activity.
abstract: PADMA 28, a natural herbal multi-compound remedy originates from traditional Tibetan medicine and possesses a variety of beneficial effects on experimental and clinical models of inflammation and atherosclerosis, as well as angioprotecive, antioxidative and wound-healing properties. The aim of the present study was to evaluate the in vivo influence of this remedy on the in vitro mitogen-induced proliferation of murine splenic lymphocytes and their chemokinetic activity in cell culture.The study was performed on 6-8 weeks old inbred Balb/c mice. PADMA28 was administered to mice per os in daily doses 5.8 mg (calculated from the highest dose recommended for human) or 0.085 mg (dose from the range of active doses of other herbal extracts containing polyphenolic substances used previously by us in experiments with mice), for 7 days. Control groups received water.
  
  
  RESULTS
  No substantial differences were observed between groups of mice fed with low and high PADMA doses. In both of them, response of splenic lymphocztes to mitogen PHA (p < 0.001) and their in vitro chemokinetic activity (p < 0.001 for low dose and p < 0.01 for high dose) were highly significantly increased as compared to the controls.
  
  
  CONCLUSIONS
  The results of our investigations suggest that PADMA 28 can stimulate cell-mediated immunity in mice and might be used for this purpose in the wide spectrum of doses.
authors.affiliation: Department of Histology and Embryology, Center for Biostructure Research, Warsaw Medical University, 02-004, Chalubińskiego 5, Warsaw, Poland.
authors.forename: P
authors.initials: P
authors.lastname: Skopiński
authors.affiliation: Department of Histology and Embryology, Center for Biostructure Research, Warsaw Medical University, 02-004, Chalubińskiego 5, Warsaw, Poland.
authors.forename: D M
authors.initials: DM
authors.lastname: Radomska-Leśniewska
authors.affiliation: Transfusion Immunology Laboratory with Blood Bank, Children's Memorial Health Institute, Al. Dzieci Polskich 20, 04-730 Warsaw, Poland.
authors.forename: I
authors.initials: I
authors.lastname: Sokolnicka
authors.affiliation: Department of Immunology, Biochemistry and Nutrition, Warsaw Medical University, Banacha 1, 02-097, Warsaw, Poland.
authors.forename: B J
authors.initials: BJ
authors.lastname: Balan
authors.affiliation: Department of Microbiology and Clinical Immunology, Warmian-Mazurian University, Oczapowskiego 13, 10-957 Olsztyn, Poland.
authors.forename: A K
authors.initials: AK
authors.lastname: Siwicki
authors.affiliation: Pathology Department, Center for Biostructure Research, Warsaw Medical University, 02-004 Chalubińskiego 5, Warsaw, Poland.
authors.forename: E
authors.initials: E
authors.lastname: Skopińska-Rózewska
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects*; D051379:Mice; D008934:Mitogens / Q000633:toxicity*; D010936:Plant Extracts / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology*
chemical_list: D008934:Mitogens; D010936:Plant Extracts; C066069:Padma 28