title: Multi-scale finite element analyses for stress and strain evaluations of braid fibril artificial blood vessel and smooth muscle cell.
abstract: In this study, we developed a multi-scale finite element (FE) analysis code to obtain the stress and strain that occurred in the smooth muscle cell (SMC) at micro-scale, which was seeded in the real fabricated braid fibril artificial blood vessel. This FE code can predict the dynamic response of stress under the blood pressure loading. We try to establish a computer-aided engineering (CAE)-driven scaffold design technique for the blood vessel regeneration. Until now, there occurred the great progresses for the endothelial cell activation and intima layer regeneration in the blood vessel regeneration study. However, there remains the difficulty of the SMC activation and media layer regeneration. Therefore, many researchers are now studying to elucidate the fundamental mechanism of SMC activation and media layer regeneration by using the biomechanical technique. As the numerical tool, we used the dynamic-explicit FE code PAM-CRASH, ESI Ltd. For the material models, the nonlinear viscoelastic constitutive law was adapted for the human blood vessel, SMC and the extra-cellular matrix, and the elastic law for the polyglycolic acid (PGA) fiber. Through macro-FE and micro-FE analyses of fabricated braid fibril tubes by using PGA fiber under the combined conditions of the orientation angle and the pitch of fiber, we searched an appropriate structure for the stress stimulation for SMC functionalization. Objectives of this study are indicated as follows: 1. to analyze the stress and strain of the human blood vessel and SMC, and 2. to calculate stress and strain of the real fabricated braid fibril artificial blood vessel and SMC to search an appropriate PGA fiber structure under combined conditions of PGA fiber numbers, 12 and 24, and the helical orientation angles of fiber, 15, 30, 45, 60, and 75 degrees. Finally, we found a braid fibril tube, which has an angle of 15 degree and 12 PGA fibers, as a most appropriate artificial blood vessel for SMC functionalization.
authors.affiliation: Department of Biomedical Engineering, Doshisha University, 1-3 Tatara-Miyakodani, Kyotanabe, Kyoto, 610-0394, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Nakamachi
authors.forename: Takahiro
authors.initials: T
authors.lastname: Uchida
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kuramae
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Morita
mesh_terms: D001696:Biomechanical Phenomena; D001794:Blood Pressure; D001807:Blood Vessel Prosthesis; D001808:Blood Vessels / Q000502:physiology*; D004548:Elasticity; D020342:Finite Element Analysis; D006801:Humans; D008422:Materials Testing; D008954:Models, Biological; D032389:Myocytes, Smooth Muscle / Q000502:physiology*; D017711:Nonlinear Dynamics; D009716:Numerical Analysis, Computer-Assisted; D013314:Stress, Mechanical; D014783:Viscosity; D055114:X-Ray Microtomography
keywords: artificial blood vessel; cell functionalization; finite element method; multi-scale finite element analysis; nonlinear viscoelasticity; smooth muscle cell