title: Correlation of the Rac1/RhoA pathway with ezrin expression in osteosarcoma.
abstract: Osteosarcoma is the most common malignant tumor of the bone. The major cause of death in osteosarcoma is the increase in metastatic potential, and the ezrin expression has been correlated with the metastasis development. Ezrin interacts with RhoGDI by dissociating it from RhoGTPases, which allow GTPases to load with GTP, activate RhoA to increase cell migration, and invasion. RhoGTPases have been found to contribute to pathologic processes including cancer cell migration, invasion, and metastasis and overexpression of either the GTPase itself or some elements of Rho signaling that have been detected in many human tumors, including Rac1 and RhoA. We have analyzed Rac1 and RhoA expression in the osteosarcoma tissues to understand the role of the ezrin-Rho family pathway in osteosarcoma metastatic progression. Moreover, we have blocked the ezrin expression using siRNA assay to investigate a possible correlation with RAC1 and RHOA expression in the osteosarcoma cell lines. Our immunohistochemical data showed that many osteosarcomas presented cytoplasmatic positivity for both Rac1 and RhoA and cases, both ezrin positive than ezrin negative, revealed the protein expression of Rac1 and RhoA. The results obtained by ezrin siRNA transfection showed that ezrin expression in the osteosarcoma cell lines might modulate, mainly, the Rac1 expression. It is possible that the mechanism of cell motility mediated by Rac1 and RhoA is maintained in osteosarcomas, and since the expression of ezrin, Rac1 and RhoA do not correlate with metastatic progression in osteosarcoma. However, osteosarcomas without metastasis displayed a positivity for Rac1 and RhoA expression compared with metastatic osteosarcomas and this could be a protective factor.
authors.affiliation: Department of Medical Surgical Sciences and Biotechnologies, Pathology Unit, ICOT, Sapienza University of Rome, Latina, Italy.
authors.forename: Caterina
authors.initials: C
authors.lastname: Chiappetta
authors.forename: Martina
authors.initials: M
authors.lastname: Leopizzi
authors.forename: Fabiana
authors.initials: F
authors.lastname: Censi
authors.forename: Chiara
authors.initials: C
authors.lastname: Puggioni
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Petrozza
authors.forename: Carlo D
authors.initials: CD
authors.lastname: Rocca
authors.forename: Claudio
authors.initials: C
authors.lastname: Di Cristofano
mesh_terms: D001859:Bone Neoplasms / Q000175:diagnosis*; D063646:Carcinogenesis / Q000235:genetics; D045744:Cell Line, Tumor; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D005500:Follow-Up Studies; D006801:Humans; D007150:Immunohistochemistry; D009362:Neoplasm Metastasis / Q000235:genetics; D012516:Osteosarcoma / Q000175:diagnosis*; D011379:Prognosis; D034741:RNA, Small Interfering / Q000235:genetics; D016019:Survival Analysis; D046888:Tissue Array Analysis; D020830:rac1 GTP-Binding Protein / Q000378:metabolism*; D020742:rhoA GTP-Binding Protein / Q000378:metabolism*; D000099149:Ezrin
chemical_list: D003598:Cytoskeletal Proteins; D034741:RNA, Small Interfering; D020830:rac1 GTP-Binding Protein; D020742:rhoA GTP-Binding Protein; D000099149:Ezrin