title: Neuroprotective effect of diphenyl diselenide in a experimental stroke model: maintenance of redox system in mitochondria of brain regions.
abstract: Acute stroke is a major risk for morbidity and mortality in aging population. Mitochondrion has been the focus of a wide stroke-related research. This study investigated if treatment or pre-treatment with diphenyl diselenide (PhSe)2 can prevent mitochondrial damage in cerebral structures of rats induced by an ischemia and reperfusion (I/R) model. Adult male Wistar rats were assigned into five experimental groups: sham operation, ischemia/reperfusion, pre-treated + I/R, treated + I/R, and Sham + (PhSe)2. Neurological score showed the damage caused by I/R, which was partially prevented by (PhSe)2. Moreover, mitochondria of hippocampus and cortex were impaired by I/R through an increase of reactive oxygen species production, mitochondrial membrane potential (ΔΨm) and electrons flow alteration, activity of complex I deregulation as well as mitochondrial swelling. However, the ischemic damage did not induce an increase in pro-apoptotic proteins expression, but demonstrated an enhanced expression of Hsp70. The mitochondrial redox state was also altered (GSH/GSSG ratio, MnSOD, and GPx activities). Our results revealed that all treatments with (PhSe)2 significantly reduced the mitochondrial damage induced by I/R. These findings suggest that neuroprotective properties of (PhSe)2 may be attributed to the maintenance of mitochondrial redox balance.
authors.affiliation: Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus UFSM, Santa Maria, RS, 97105-900, Brazil.
authors.forename: Fernando
authors.initials: F
authors.lastname: Dobrachinski
authors.forename: Michele Hinerasky
authors.initials: MH
authors.lastname: da Silva
authors.forename: Cíntia Letícia Cardias
authors.initials: CL
authors.lastname: Tassi
authors.forename: Nélson Rodrigues
authors.initials: NR
authors.lastname: de Carvalho
authors.forename: Glaecir Roseni Mundstock
authors.initials: GR
authors.lastname: Dias
authors.forename: Ronaldo Medeiros
authors.initials: RM
authors.lastname: Golombieski
authors.forename: Elgion Lúcio
authors.initials: EL
authors.lastname: da Silva Loreto
authors.forename: João Batista Teixeira
authors.initials: JB
authors.lastname: da Rocha
authors.forename: Michele Rechia
authors.initials: MR
authors.lastname: Fighera
authors.forename: Félix Alexandre Antunes
authors.initials: FA
authors.lastname: Soares
mesh_terms: D000818:Animals; D001555:Benzene Derivatives / Q000494:pharmacology*; D002545:Brain Ischemia; D002540:Cerebral Cortex / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D004195:Disease Models, Animal; D005978:Glutathione / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016566:Organoselenium Compounds / Q000494:pharmacology*; D010088:Oxidoreductases / Q000378:metabolism; D011897:Random Allocation; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury; D012720:Severity of Illness Index; D020521:Stroke / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D001555:Benzene Derivatives; D018840:HSP70 Heat-Shock Proteins; D018696:Neuroprotective Agents; D016566:Organoselenium Compounds; D017382:Reactive Oxygen Species; C061132:diphenyldiselenide; D010088:Oxidoreductases; D013482:Superoxide Dismutase; D005978:Glutathione