title: Detection methods of immunoglobulin IgG in CSF and serum.
abstract: BACKGROUND
  This study represents a new approach to the extended analysis of correlation of findings of oligoclonal bands on gels and the level of intrathecal synthesis of immunoglobulin G in the central nervous system. Previous studies have shown that there is no correlation at this level as well as the number of tape or finding does not correlate with the forecast effect of therapy or patient outcome.
  
  
  OBJECTIVE
  To determine the correlation of level of immunoglobulins IgG in CSF with the number of oligoclonal bands on the gel.
  
  
  METHODS
  The retrospective study based on data processed in Clinical Immunology Clinical Center University of Sarajevo. Patients were assumed of multiple sclerosis according to clinical findings and magnetic resonance imaging. All CSF and serum samples were processed by nephelometry, isoelectric focusing on the gel. Statistical analysis of results was also performed by using SPSS statistical analysis program.
  
  
  RESULTS
  Analyses were performed on 254 samples of cerebrospinal fluid and serum of patients from neurological clinic, suspected of multiple sclerosis. We concluded that there is no correlation between the level of intrathecal synthesis obtained by Reibergram with the number of oligoclonal bands on gels. We think that the reason could be a small sample of patients analyzed and it leaves room for future analysis on a larger sample.
  
  
  CONCLUSIONS
  For most patients with established MS we found intrathecal humoral response, type two, and the number and arrangement of IgG bands generally does not change during the disease, because they reflect long-term non-specific immune stimulation rather than a specific immune response that during infectious disease changes (quantitatively and qualitatively).
authors.affiliation: Clinical Immunology, Clinical Center of Sarajevo University, Sarajevo, Bosnia and Herzegovina. marinadelicsarac@gmail.com
authors.forename: Marina
authors.initials: M
authors.lastname: Delic-Sarac
authors.affiliation: General Hospital "Prim. Dr. Abdulah Nakas", Sarajevo, Bosnia and Herzegovina.
authors.forename: Sebija
authors.initials: S
authors.lastname: Izetbegovic
authors.affiliation: Clinical Immunology, Clinical Center of Sarajevo University, Sarajevo, Bosnia and Herzegovina.
authors.forename: Jasenko
authors.initials: J
authors.lastname: Karamehic
authors.affiliation: Clinical Immunology, Clinical Center of Sarajevo University, Sarajevo, Bosnia and Herzegovina.
authors.forename: Djemo
authors.initials: D
authors.lastname: Subasic
authors.affiliation: Faculty of Stomatology and Pharmacy, University of Travnik, Travnik, Bosnia and Herzegovina.
authors.forename: Aida
authors.initials: A
authors.lastname: Saban
authors.affiliation: Scientific Department of Biomedicine and Health, Faculty of medicine, University of Osijek, Osijek, Croatia.
authors.forename: Tomislav
authors.initials: T
authors.lastname: Jukic
authors.affiliation: Clinical Biochemistry, Clinical Center of Sarajevo University, Sarajevo, Bosnia and Herzegovina.
authors.forename: Jozo
authors.initials: J
authors.lastname: Coric
authors.affiliation: Clinical Biochemistry, Clinical Center of Sarajevo University, Sarajevo, Bosnia and Herzegovina.
authors.forename: Mirsad
authors.initials: M
authors.lastname: Panjeta
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D004586:Electrophoresis / Q000379:methods; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000134:cerebrospinal fluid / Q000276:immunology; D007525:Isoelectric Focusing / Q000379:methods; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000175:diagnosis* / Q000276:immunology; D009391:Nephelometry and Turbidimetry / Q000379:methods; D043025:Oligoclonal Bands / Q000276:immunology*; D055815:Young Adult
chemical_list: D007074:Immunoglobulin G; D043025:Oligoclonal Bands