title: The effects of different mechanisms on the development of post-ERCP pancreatitis in an ERCP model in rats.
abstract: OBJECTIVE
  To investigate the effects of different mechanisms on the development of pancreatitis after endoscopic retrograde cholangiopancreatography.
  
  
  METHODS
  40 male rats were randomly divided into four groups. After laparotomy, in Group 1, only duodenum was reached by a 24G cannula without performing any other procedure. In Groups 2, 3, and 4, biliopancreatic duct was cannulated transduodenally. Group 2 received no additional intervention after the cannulation. Group 3 received saline, whereas Group 4 received contrast agent into the duct. After a period of 24 hours, all rats were sacrificed. Laboratorytests for blood samples were performed and pancreatic tissue was also evaluated histopathologically.
  
  
  RESULTS
  Leukocyte, blood sugar, serum glutamic oxaloacetic transaminase, lactate dehydrogenase, amylase, C-reactive protein, and base excess parameters were evaluated. The values in Groups 2, 3, and 4 were found to be significantly higher than those in the control group, except for leukocyte count and base excess (p=0.551, p=0.031, p=0.0001, p=0.0001, p=0.0001, p=0.0001, p=0.683, respectively). Histopathological results demonstrated significant differences between the groups. Highest pathological damage scores were observed in Groups 3 and 4.
  
  
  CONCLUSIONS
  Among different theories for the pathogenesis of post-endoscopic retrograde cholangiopancreatography pancreatitis, elevated intraductal hydrostatic pressure was observed to be the main underlying cause.
authors.affiliation: Bezmialem Vakıf University School of Medicine, Department of General Surgery, Fatih, İstanbul, Turkey.
authors.forename: Süleyman
authors.initials: S
authors.lastname: Bozkurt
authors.forename: Ali
authors.initials: A
authors.lastname: Güner
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Kadıoğlu
authors.forename: Can
authors.initials: C
authors.lastname: Keçe
authors.forename: Erhan
authors.initials: E
authors.lastname: Reis
authors.forename: Halil
authors.initials: H
authors.lastname: Coşkun
mesh_terms: D000137:Acid-Base Imbalance / Q000097:blood; D000681:Amylases / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001652:Bile Ducts; D001786:Blood Glucose / Q000378:metabolism; D002097:C-Reactive Protein / Q000378:metabolism; D002404:Catheterization / Q000009:adverse effects; D002760:Cholangiopancreatography, Endoscopic Retrograde / Q000009:adverse effects*; D003287:Contrast Media / Q000008:administration & dosage / Q000009:adverse effects; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007958:Leukocyte Count; D008297:Male; D023421:Models, Animal; D010183:Pancreatic Ducts; D010195:Pancreatitis / Q000097:blood / Q000209:etiology* / Q000473:pathology; D011897:Random Allocation; D017208:Rats, Wistar; D012965:Sodium Chloride / Q000008:administration & dosage / Q000009:adverse effects
chemical_list: D001786:Blood Glucose; D003287:Contrast Media; D012965:Sodium Chloride; D002097:C-Reactive Protein; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000681:Amylases