title: NRP-1 expression in bladder cancer and its implications for tumor progression.
abstract: Neuropilin-1 (NRP-1) overexpression has been reported in a variety of human cancers. However, the role of NRP-1 in bladder cancer (BC) remains unclear. The aim of present study was to analyze NRP-1 protein expression in BC tissues and to assess its prognostic significance for BC. NRP-1 messenger ribonucleic acid (mRNA) and protein expression were determined by real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) and immunohistochemistry in specimens of primary cancer and their adjacent noncancerous tissues in BC patients. Additionally, NRP-1 protein expression in 139 archived paraffin-embedded BC samples was analyzed by immunohistochemistry and correlated with clinicopathological characteristics and survival. Student's t test, Spearman's rank correlation, Kaplan-Meier plots, and Cox's proportional hazards regression model were used to analyze the data. By qRT-PCR and immunohistochemistry, the levels of NRP-1 mRNA and protein were significantly higher in BC, compared to that in adjacent noncancerous tissues (P<0.001). High expression of NRP-1 was significantly associated with histologic grade (P=0.016) and tumor stage (P=0.001). Multivariate analysis showed that high expression of NRP-1 was an independent prognostic factor for overall survival. Our study suggests that overexpression of NRP-1 may play an important role in the progression of BC, and NRP-1 expression may serve as a biomarker for poor prognosis for BC.
authors.affiliation: Department of Urology, Nanjing Jinling Hospital, Nanjing University School of Medicine, No. 305, Zhongshandong Road, Nanjing, 210002, China, chengwen2007@126.com.
authors.forename: Wen
authors.initials: W
authors.lastname: Cheng
authors.forename: Dian
authors.initials: D
authors.lastname: Fu
authors.forename: Zhi-Feng
authors.initials: ZF
authors.lastname: Wei
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Xu
authors.forename: You-Huang
authors.initials: YH
authors.lastname: Liu
authors.forename: Jing-Ping
authors.initials: JP
authors.lastname: Ge
authors.forename: Feng
authors.initials: F
authors.lastname: Tian
authors.forename: Cong-Hui
authors.initials: CH
authors.lastname: Han
authors.forename: Zheng-Yu
authors.initials: ZY
authors.lastname: Zhang
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Zhou
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor; D018450:Disease Progression; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D039942:Neuropilin-1 / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D016016:Proportional Hazards Models; D001749:Urinary Bladder Neoplasms / Q000209:etiology* / Q000401:mortality / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D039942:Neuropilin-1