title: [Pseudomonas aeruginosa recombinant proteins: effect on mice cytokine profile].
abstract: OBJECTIVE
  Study cytokine-mediated immune response in mice vaccinated with Pseudomonas aeruginosa recombinant antigen preparations.
  
  
  METHODS
  Cytokine-mediated immune response was studied in mice vaccinated with membrane recombinant proteins OprF, OprL, a hybrid recombinant protein OprF-I consisting of sequences of OprF and OprI proteins and a recombinant atoxic form of exotoxin A with a deletion of 106 amino acid sequences (recombinant anatoxin - aTox) of P. aeruginosa.
  
  
  RESULTS
  An induction of a wide specter of studied cytokines was detected in the mice. The highest level was observed for IL-1 and IL-6 after administration of recombinant proteins OprL, OprF, OprF-1, aTox. OprF-I actively stimulated production of IL-2 that is a factor of growth and differentiation of lymphocytes, natural killers and cytotoxic lymphocytes; as well as IL-5, IL-O10, TNF-alpha and IFN-gamma. Recombinant protein OprF-I facilitated induction of IL-6, IL-17, TNF-alpha and IFN-gamma, whereas aTox - expression of IL-1, IL-2, IFN-gamma. Recombinant protein OprL induced IL-17 synthesis to the most extent and TNF-alpha and IL-10 - moderately.
  
  
  CONCLUSIONS
  The P. aeruginosa recombinant proteins obtained during intraperitoneal administration to mice facilitated formation of immune response with the direction of induction in both Thl and Th2 pathways.
authors.forename: A V
authors.initials: AV
authors.lastname: Soldatenkova
authors.forename: L A
authors.initials: LA
authors.lastname: Geĭderova
authors.forename: N K
authors.initials: NK
authors.lastname: Akhmatova
authors.forename: N A
authors.initials: NA
authors.lastname: Mikhaĭlova
mesh_terms: D036002:ADP Ribose Transferases / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D016207:Cytokines / Q000276:immunology*; D005098:Exotoxins / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D007111:Immunity, Cellular / Q000187:drug effects*; D008074:Lipoproteins / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D051379:Mice; D008808:Mice, Inbred CBA; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D037521:Virulence Factors / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D000097668:Pseudomonas aeruginosa Exotoxin A
chemical_list: D036002:ADP Ribose Transferases; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D016207:Cytokines; D005098:Exotoxins; D008074:Lipoproteins; D011993:Recombinant Fusion Proteins; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A; C547605:OprF protein, Pseudomonas aeruginosa; C060507:outer membrane lipoprotein I, Bacteria