title: Urinary 1-hydroxypyrene and 8-hydroxydeoxyguanosine levels among coke-oven workers for 2 consecutive days.
abstract: OBJECTIVE
  This study evaluated the levels of exposure to polycyclic aromatic hydrocarbons (PAHs) and their relationship with oxidative DNA damage among Vietnamese coke-oven workers.
  
  
  METHODS
  We collected urine from 36 coke-oven workers (exposed group) at the beginning and end of the shift on 2 consecutive days. We also collected urine from 78 medical staff (control group). Information was collected by questionnaire about smoking status, drinking habit, and working position. Urinary 1-hydroxypyrene (1-OHP) and 8-hydroxydeoxyguanosine (8-OH-dG) were measured using HPLC. All statistical analyses were performed with SPSS version 19.
  
  
  RESULTS
  Urinary 1-OHP was significantly higher in the coke-oven workers than in the control group (p<0.05). Top-oven workers had the highest levels of internal exposure to PAHs, followed by side-oven and then bottom-oven workers (5.41, 4.41 and 1.35 ng/mg creatinine, respectively, at the end of the shift on day 2). Urinary 8-OH-dG was significantly higher in top- and side-oven workers at the end of the shift on day 2 (4.63 and 5.88 ng/mg creatinine, respectively) than in the control group (3.85 ng/mg creatinine). Based on a multi-regression analysis, smoking status had a significant effect on urinary 8-OH-dG (p=0.049). Urinary 1-OHP tended to have a positive correlation with urinary 8-OH-dG (p=0.070).
  
  
  CONCLUSIONS
  Vietnamese coke-oven workers were exposed to PAHs during their work shift. Urinary 1-OHP exceeded the recommended limit, and elevated oxidative DNA damage occurred in top- and side-oven workers on the second day of work. A tendency for positive correlation was found between urinary 1-OHP and urinary 8-OH-dG.
authors.affiliation: Department of Health Policy and Management, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health.
authors.forename: Thi-To-Uyen
authors.initials: TT
authors.lastname: Nguyen
authors.forename: Shoko
authors.initials: S
authors.lastname: Kawanami
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Kawai
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kasai
authors.forename: Yun-Shan
authors.initials: YS
authors.lastname: Li
authors.forename: Jinro
authors.initials: J
authors.lastname: Inoue
authors.forename: Le Tran
authors.initials: le T
authors.lastname: Ngoan
authors.forename: Seichi
authors.initials: S
authors.lastname: Horie
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000395:Air Pollutants, Occupational / Q000032:analysis; D016022:Case-Control Studies; D003077:Coke; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000633:toxicity / Q000652:urine; D005260:Female; D006801:Humans; D007221:Industry; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure / Q000032:analysis*; D018384:Oxidative Stress; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity; D011721:Pyrenes / Q000633:toxicity / Q000652:urine*; D014744:Vietnam
chemical_list: D000395:Air Pollutants, Occupational; D003077:Coke; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; C033146:1-hydroxypyrene