title: Frequency of sabA Gene in Helicobacter pylori Strains Isolated From Patients in Tehran, Iran.
abstract: BACKGROUND
  The importance of sialic acid binding adhesin (sabA) as a new outer membrane protein in gastroduodenal diseases has been recognized. The prevalence rate of sabA gene varies in different geographic areas.
  
  
  OBJECTIVE
  The aim of this study was to determine the frequency of sabA gene in Helicobacter pylori (H. pylori) strains isolated from different clinical outcomes in Tehran, Iran.
  
  
  METHODS
  The study included 120 patients with dyspeptic symptoms admitted to the endoscopy suite of gastroenterology section of Firouzgar University Hospital, Tehran, Iran from March to August 2011. Gastric biopsy specimens were evaluated for the presence of H. pylori using standard microbiological method and polymerase chain reaction (PCR) assay. The sabA genopositive was determined by PCR in H. pylori strains.
  
  
  RESULTS
  H. pylori isolates were recovered from 82 patients with duodenal ulcer (DU; n = 17), gastric ulcer (GU; n = 15), gastric cancer (GC; n = 13), and gastritis (G; n = 37). The frequency of sabA gene in H. pylori strains was 100% in gastric cancer, 86.7% in gastric ulcer, and 83.3% in both gastritis and duodenal ulcer.
  
  
  CONCLUSIONS
  This is a report on the prevalence of sabA gene in H. pylori isolated from different gastric patients in Iran. The results showed a high prevalence of sabA in our clinical H. pylori isolates.
authors.affiliation: Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Pakbaz
authors.affiliation: Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Mohammad Hasan
authors.initials: MH
authors.lastname: Shirazi
authors.affiliation: Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Ranjbar
authors.affiliation: Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Pourmand
authors.affiliation: Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Khalifeh Gholi
authors.affiliation: Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Aliramezani
authors.affiliation: Master of Sciences, Microbial Biotechnology.
authors.forename: Ziba
authors.initials: Z
authors.lastname: Vaise Malekshahi
keywords: Gastric Ulcer; Gene Frequency; SabA protein, Helicobacter pylori