title: Molecularly imprinted polymer cartridges coupled on-line with high performance liquid chromatography for simple and rapid analysis of human insulin in plasma and pharmaceutical formulations.
abstract: In this paper, a novel method is described for automated determination of human insulin in biological fluids using principle of sequential injection on a molecularly imprinted solid-phase extraction (MISPE) cartridge as a sample clean-up technique combined with high performance liquid chromatography (HPLC). The water-compatible molecularly imprinted polymers (MIPs) were prepared using methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a cross-linker, chloroform as a porogen and insulin as a template molecule. The imprinted polymers were then employed as the solid-phase extraction sorbent for on-line extraction of insulin from human plasma samples. To achieve the best condition, influential parameters on the extraction efficiency were thoroughly investigated. Rapid and simple analysis of the hormone was successfully accomplished through the good selectivity of the prepared sorbent coupled with HPLC. Limits of detection (LOD) and quantification (LOQ) of 0.2 ng mL(-1), 0.7 ng mL(-1), and 0.03 ng mL(-1), 0.1 ng mL(-1) were obtained in plasma and urine respectively. The obtained data exhibited the great recoveries for extraction of insulin from human plasma and pharmaceutical samples, higher than 87%.
authors.affiliation: Department of Chemistry, Amirkabir University of Technology, Tehran, Iran.
authors.forename: Mohammad Mahdi
authors.initials: MM
authors.lastname: Moein
authors.affiliation: Department of Chemistry, Amirkabir University of Technology, Tehran, Iran. Electronic address: mehranjavanbakht@gmail.com.
authors.forename: Mehran
authors.initials: M
authors.lastname: Javanbakht
authors.affiliation: Food & Drug Laboratory Research Center, Food & Drug Organization, Ministry of Health and Medical Education, Tehran, Iran.
authors.forename: Behrouz
authors.initials: B
authors.lastname: Akbari-adergani
mesh_terms: D000327:Adsorption; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000032:analysis / Q000097:blood*; D057230:Limit of Detection; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000295:instrumentation*
chemical_list: D007328:Insulin; D004364:Pharmaceutical Preparations
keywords: Automated; Human plasma; Insulin; Molecularly imprinted polymer; Pharmaceutical formulations; Sample clean-up