title: Sphingosine analog AAL-R promotes activation of LCMV-infected dendritic cells.
abstract: Sphingosine analogs display diverse immunoregulatory activities with curative potential in autoimmune diseases and viral infections. Recently, the sphingosine analog AAL-R was shown to increase DC activation upon TLR7 stimulation. Here, we investigated the effect of AAL-R on activation of dendritic cells (DCs) infected by lymphocytic choriomeningitis virus (LCMV). Concomitant treatment of LCMV-infected DCs with AAL-R enhanced DC maturation and DC ability to stimulate and expand antiviral CD8(+) T cells. Importantly, AAL-R's stimulatory activity was abrogated in type I interferon (IFN) receptor-deficient DCs following LCMV infection. In support of this observation, AAL-R increased type I IFN production from DCs infected with LCMV. Taken together, the sphingosine analog could directly act on DCs to promote defensive host DC responses to the viral invasion via type I IFN signaling.
authors.affiliation: Departments of Surgery and Molecular Microbiology and Immunology, Center for Cellular and Molecular Immunology, University of Missouri-Columbia , Columbia, Missouri.
authors.forename: Young-Jin
authors.initials: YJ
authors.lastname: Seo
authors.forename: Bumsuk
authors.initials: B
authors.lastname: Hahm
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D049109:Cell Proliferation; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology*; D007155:Immunologic Factors / Q000276:immunology* / Q000378:metabolism; D007370:Interferon Type I / Q000276:immunology; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D013110:Sphingosine / Q000031:analogs & derivatives / Q000276:immunology* / Q000378:metabolism
chemical_list: D007155:Immunologic Factors; D007370:Interferon Type I; D013110:Sphingosine