title: [Formation of focus of West Nile fever on the territory of Saratov Region].
abstract: OBJECTIVE
  Detection of circulation of West Nile virus (WNV) on the territory of Saratov Region and prerequisites for formation of natural focus of West Nile fever (WNF), determination of the role of WNV in infectious pathology on the territory of the region.
  
  
  METHODS
  of organs of small mammals, birds, blood-sucking arthropods for the presence of WNV markers (antigens and/or RNA) were studied. Clinical material from patients with symptoms not excluding WNF was studied. Donor blood sera samples were analyzed with the aim of detection of immune layer against WNV in population of Saratov Region.
  
  
  RESULTS
  In 2010 WNV antigens were detected by EIA in 12 samples (7.1%) of mammal organ suspensions. In 2012 by using RT-PCR and EIA, markers of WNV were detected in 6 samples of bird brain suspensions (6.3%) and 1 sample of mammal organ suspension. Immune layer of population against WNV was 4% in 2011, 2.8% in 2012. In 2012 in 11 of 27 examined patients IgM against WNV in diagnostic titers and/or serconversion of IgG in paired sera were detected. In addition in 5 individuals virus RNA was detected in blood. Based on clinical, laboratory data and epidemiologic anamnesis 11 patients were diagnosed with WNF.
  
  
  CONCLUSIONS
  The results obtained give evidence on the circulation of WNV on the territory of Saratov Region in 2010 - 2012. With the development of complications of WNF epidemiologic situation in 2012 an expansion of WNV areal onto the territory of the region took place and the process of formation of WNF natural foci is ongoing.
authors.forename: T Iu
authors.initials: TIu
authors.lastname: Krasovskaia
authors.forename: I N
authors.initials: IN
authors.lastname: Sharova
authors.forename: E V
authors.initials: EV
authors.lastname: Naĭdenova
authors.forename: V N
authors.initials: VN
authors.lastname: Chekashov
authors.forename: S A
authors.initials: SA
authors.lastname: Shcherbakova
authors.forename: E A
authors.initials: EA
authors.lastname: Bil'ko
authors.forename: V E
authors.initials: VE
authors.lastname: Kuklev
authors.forename: A N
authors.initials: AN
authors.lastname: Matrosov
authors.forename: S A
authors.initials: SA
authors.lastname: Iakovlev
authors.forename: A M
authors.initials: AM
authors.lastname: Porshakov
authors.forename: M M
authors.initials: MM
authors.lastname: Shilov
authors.forename: A A
authors.initials: AA
authors.lastname: Kuznetsov
authors.forename: T V
authors.initials: TV
authors.lastname: Kniazeva
authors.forename: A M
authors.initials: AM
authors.lastname: Senichkina
authors.forename: E V
authors.initials: EV
authors.lastname: Kazorina
authors.forename: N V
authors.initials: NV
authors.lastname: Popov
authors.forename: Z P
authors.initials: ZP
authors.lastname: Fedorova
authors.forename: U A
authors.initials: UA
authors.lastname: Kresova
authors.forename: E A
authors.initials: EA
authors.lastname: Talaeva
authors.forename: N I
authors.initials: NI
authors.lastname: Mironova
authors.forename: O I
authors.initials: OI
authors.lastname: Kozhanova
authors.forename: V V
authors.initials: VV
authors.lastname: Kutyrev
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001717:Birds / Q000097:blood / Q000821:virology; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D012426:Russia / Q000453:epidemiology; D014901:West Nile Fever / Q000097:blood / Q000453:epidemiology / Q000635:transmission; D014902:West Nile virus
chemical_list: D000914:Antibodies, Viral; D007075:Immunoglobulin M