title: Prevalence of Helicobacter pylori vacA different genotypes in Isfahan, Iran.
abstract: BACKGROUND
  It is believed that the Helicobacter pylori (H. pylori) vacA gene, as a major virulence determinant (One of the major virulence determinant, not major), may be a risk factor for the development of gastroduodenal diseases. The frequency of vacA genotypes varies in different human populations. This study evaluated the prevalence of vacA alleles/genotypes among dyspeptic patients in Isfahan.
  
  
  METHODS
  One-hundred H. pylori-positive adult patients were examined in this study. After culture of gastric biopsies and DNA extraction from individual H. pylori isolates, the (all H. pylori strains harbor vacA alleles, please replace "presence" with "genotypes") of the vacA s and m alleles were determined using polymerase chain reaction (PCR).
  
  
  RESULTS
  There were four vacA mosaicisms, including 28 for s1a/m1 (28%), 23 for s1b/m1 (23%), 26 for s1a/m2 (26%) and 23 for s1b/m2 (23%). The s2 allele was not found. The predominant vacA genotype in patients with non-ulcer dyspepsia and duodenal ulcer was s1a/m2, whereas in patients with adenocarcinoma was s1a/m1.
  
  
  CONCLUSIONS
  The results showed there was no significant correlation between different genotypes of the vacA and the clinical outcomes and appears to vacA genotypes were not useful determinants for gastrointestinal diseases in our area.
authors.affiliation: Department of Microbiology, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran.
authors.forename: S Asghar
authors.initials: SA
authors.lastname: Havaei
authors.affiliation: Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Parviz
authors.initials: P
authors.lastname: Mohajeri
authors.affiliation: Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Khashei
authors.affiliation: Department of Cell Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Rasoul
authors.initials: R
authors.lastname: Salehi
authors.affiliation: Department of Gastroenterology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Tavakoli
keywords: Adenocarsinoma; Helicobacter pylori; Iran; Peptic ulcere; gastroduodenal diseases; vacA gene