title: Polyhedra self-assembled from DNA tripods and characterized with 3D DNA-PAINT.
abstract: DNA self-assembly has produced diverse synthetic three-dimensional polyhedra. These structures typically have a molecular weight no greater than 5 megadaltons. We report a simple, general strategy for one-step self-assembly of wireframe DNA polyhedra that are more massive than most previous structures. A stiff three-arm-junction DNA origami tile motif with precisely controlled angles and arm lengths was used for hierarchical assembly of polyhedra. We experimentally constructed a tetrahedron (20 megadaltons), a triangular prism (30 megadaltons), a cube (40 megadaltons), a pentagonal prism (50 megadaltons), and a hexagonal prism (60 megadaltons) with edge widths of 100 nanometers. The structures were visualized by means of transmission electron microscopy and three-dimensional DNA-PAINT super-resolution fluorescent microscopy of single molecules in solution.
authors.affiliation: Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Iinuma
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Ke
authors.forename: Ralf
authors.initials: R
authors.lastname: Jungmann
authors.forename: Thomas
authors.initials: T
authors.lastname: Schlichthaerle
authors.forename: Johannes B
authors.initials: JB
authors.lastname: Woehrstein
authors.forename: Peng
authors.initials: P
authors.lastname: Yin
mesh_terms: D004247:DNA / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D008970:Molecular Weight; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology; D009690:Nucleic Acid Conformation
chemical_list: D004247:DNA