title: Virus-induced gene silencing using a modified betasatellite: a potential candidate for functional genomics of crops.
abstract: Betasatellites are geminivirus-associated single-stranded DNA molecules that play an important role in symptom modulation. A VIGS vector was developed by modifying cotton leaf curl Multan betasatellite (CLCuMB). CLCuMB DNA was modified by replacing the βC1 gene with a multiple cloning site. The silencing ability of the modified CLCuMB was investigated by cloning a fragment of a host gene (Su) or a reporter transgene (uidA) into the modified CLCuMB and co-agroinoculation with cotton leaf curl Multan virus, cotton leaf curl Kokhran virus, and ageratum enation virus, separately. The inoculated Nicotiana tabacum, N. benthamiana, Solanum lycopersicum, Arabidopsis thaliana and Gossypium hirsutum plants showed efficient silencing of the cognate genes.
authors.affiliation: National Agri-Food Biotechnology Institute, Mohali, 160071, Punjab, India, jitsingh27281@gmail.com.
authors.forename: Jitendra
authors.initials: J
authors.lastname: Kumar
authors.forename: Samatha
authors.initials: S
authors.lastname: Gunapati
authors.forename: Jitesh
authors.initials: J
authors.lastname: Kumar
authors.forename: Anita
authors.initials: A
authors.lastname: Kumari
authors.forename: Abhinav
authors.initials: A
authors.lastname: Kumar
authors.forename: Rakesh
authors.initials: R
authors.lastname: Tuli
authors.forename: Sudhir P
authors.initials: SP
authors.lastname: Singh
mesh_terms: D053839:Begomovirus / Q000235:genetics; D018556:Crops, Agricultural / Q000235:genetics / Q000821:virology; D020868:Gene Silencing; D005822:Genetic Vectors / Q000235:genetics* / Q000378:metabolism; D003368:Gossypium / Q000235:genetics* / Q000821:virology; D018551:Solanum lycopersicum / Q000235:genetics* / Q000821:virology; D010935:Plant Diseases / Q000235:genetics* / Q000821:virology; D012526:Satellite Viruses / Q000235:genetics* / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics* / Q000821:virology