title: Effect of cytochrome C and vitamin C on the survival of MCF-7 cancer cells under γ-ray treatment.
abstract: In the present study, the radiation-induced effect of cytochrome c (CytC) and vitamin C (VitC) is studied with respect to survival of MCF-7 cancer cells grown in aerated media. Both, CytC and VitC, were incubated with MCF-7 cancer cells under various concentrations individually, as well as in mixture and cells were subsequently treated with γ-ray in a dose range of 0 to 30 Gy. Generally, an increase of cell survival was observed under substance treatment up to a radiation dose of 5 Gy compared to the control group. Based on the obtained results it is believed that cell survival depends strongly on the action of free radicals produced at the given concentration of the incubated CytC and VitC as well as on the specific reaction rate constants (k: l mol(-1) s(-1)) of the involved processes.
authors.affiliation: University of Vienna, Faculty of Life Science, Section of Radiation Biology, Althanstr.14, A-1090 Vienna, Austria. nikola.getoff@univie.ac.at.
authors.forename: Günter
authors.initials: G
authors.lastname: Walder
authors.forename: Nikola
authors.initials: N
authors.lastname: Getoff
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D001205:Ascorbic Acid / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology* / Q000532:radiotherapy; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D045304:Cytochromes c / Q000494:pharmacology*; D005260:Female; D005609:Free Radicals / Q000378:metabolism; D005720:Gamma Rays; D006801:Humans; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D011838:Radiation-Sensitizing Agents; D045304:Cytochromes c; D001205:Ascorbic Acid
keywords: Cytochrome C; MCF-7 cells; free radicals; radiation survival; vitamin C