title: Retinal input directs the recruitment of inhibitory interneurons into thalamic visual circuits.
abstract: Inhibitory interneurons (INs) critically control the excitability and plasticity of neuronal networks, but whether activity can direct INs into specific circuits during development is unknown. Here, we report that in the dorsal lateral geniculate nucleus (dLGN), which relays retinal input to the cortex, circuit activity is required for the migration, molecular differentiation, and functional integration of INs. We first characterize the prenatal origin and molecular identity of dLGN INs, revealing their recruitment from an Otx2(+) neuronal pool located in the adjacent ventral LGN. Using time-lapse and electrophysiological recordings, together with genetic and pharmacological perturbation of retinal waves, we show that retinal activity directs the navigation and circuit incorporation of dLGN INs during the first postnatal week, thereby regulating the inhibition of thalamocortical circuits. These findings identify an input-dependent mechanism regulating IN migration and circuit inhibition, which may account for the progressive recruitment of INs into expanding excitatory circuits during evolution.
authors.affiliation: Department of Basic Neurosciences, University of Geneva, 1 Rue Michel Servet, 1211 Geneva, Switzerland.
authors.forename: Bruno
authors.initials: B
authors.lastname: Golding
authors.affiliation: Department of Basic Neurosciences, University of Geneva, 1 Rue Michel Servet, 1211 Geneva, Switzerland.
authors.forename: Gabrielle
authors.initials: G
authors.lastname: Pouchelon
authors.affiliation: Department of Basic Neurosciences, University of Geneva, 1 Rue Michel Servet, 1211 Geneva, Switzerland.
authors.forename: Camilla
authors.initials: C
authors.lastname: Bellone
authors.affiliation: Department of Basic Neurosciences, University of Geneva, 1 Rue Michel Servet, 1211 Geneva, Switzerland.
authors.forename: Sahana
authors.initials: S
authors.lastname: Murthy
authors.affiliation: Center for Interdisciplinary Research in Biology, UMR CNRS 7241/INSERM U1050, Collège de France, 11 Place Marcelin Berthelot, 75005 Paris, France.
authors.forename: Ariel A
authors.initials: AA
authors.lastname: Di Nardo
authors.affiliation: Department of Basic Neurosciences, University of Geneva, 1 Rue Michel Servet, 1211 Geneva, Switzerland.
authors.forename: Subashika
authors.initials: S
authors.lastname: Govindan
authors.affiliation: Riken Brain Science Institute, 2-1 Hirosawa Wako City, Saitama 351-0198, Japan.
authors.forename: Masahuro
authors.initials: M
authors.lastname: Ogawa
authors.affiliation: Riken Brain Science Institute, 2-1 Hirosawa Wako City, Saitama 351-0198, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Shimogori
authors.affiliation: Department of Basic Neurosciences, University of Geneva, 1 Rue Michel Servet, 1211 Geneva, Switzerland; Department of Neurology, Geneva University Hospital, 4 Rue Gabrielle-Perret-Gentil, 1205 Geneva, Switzerland.
authors.forename: Christian
authors.initials: C
authors.lastname: Lüscher
authors.affiliation: Department of Basic Neurosciences, University of Geneva, 1 Rue Michel Servet, 1211 Geneva, Switzerland; Department of Psychiatry, Geneva University Hospital, 4 Rue Gabrielle-Perret-Gentil, 1205 Geneva, Switzerland.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Dayer
authors.affiliation: Department of Basic Neurosciences, University of Geneva, 1 Rue Michel Servet, 1211 Geneva, Switzerland; Department of Neurology, Geneva University Hospital, 4 Rue Gabrielle-Perret-Gentil, 1205 Geneva, Switzerland. Electronic address: denis.jabaudon@unige.ch.
authors.forename: Denis
authors.initials: D
authors.lastname: Jabaudon
mesh_terms: D000818:Animals; D005075:Biological Evolution; D002465:Cell Movement / Q000502:physiology*; D005260:Female; D051524:Fibroblast Growth Factor 8 / Q000235:genetics / Q000378:metabolism; D059330:GABAergic Neurons / Q000166:cytology / Q000502:physiology; D005829:Geniculate Bodies / Q000166:cytology* / Q000196:embryology / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000235:genetics; D007395:Interneurons / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009433:Neural Inhibition / Q000502:physiology*; D009473:Neuronal Plasticity / Q000502:physiology; D051857:Otx Transcription Factors / Q000235:genetics / Q000378:metabolism; D011247:Pregnancy; D011978:Receptors, Nicotinic / Q000235:genetics / Q000378:metabolism; D012160:Retina / Q000166:cytology* / Q000196:embryology / Q000502:physiology; D013569:Synapses / Q000502:physiology; D014795:Visual Pathways / Q000166:cytology / Q000196:embryology / Q000502:physiology
chemical_list: D051524:Fibroblast Growth Factor 8; D049452:Green Fluorescent Proteins; D051857:Otx Transcription Factors; D011978:Receptors, Nicotinic; C496457:Fgf8 protein, mouse; C497660:Otx2 protein, mouse