title: Plastid transformation in tomato.
abstract: Tomato (Solanum lycopersicum) is one of the most important vegetable crops and has long been an important model species in plant biology. Plastid biology in tomato is especially interesting due to the chloroplast-to-chromoplast conversion occurring during fruit ripening. Moreover, as tomato represents a major food crop with an edible fruit that can be eaten raw, the development of a plastid transformation protocol for tomato was of particular interest to plant biotechnology. Recent methodological improvements have made tomato plastid transformation more efficient and facilitated applications in metabolic engineering and molecular farming. This article describes the basic methods involved in the generation and analysis of tomato plants with transgenic chloroplast genomes and summarizes current applications of tomato plastid transformation.
authors.affiliation: Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam-Golm, Germany.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Ruf
authors.forename: Ralph
authors.initials: R
authors.lastname: Bock
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D017123:Bacteriophage T7 / Q000235:genetics; D019470:Biolistics / Q000379:methods; D002736:Chloroplasts / Q000235:genetics*; D018556:Crops, Agricultural; D005822:Genetic Vectors; D019753:Intramolecular Lyases / Q000235:genetics; D018551:Solanum lycopersicum / Q000235:genetics*; D058634:Molecular Farming; D009713:Nucleotidyltransferases / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics; D011401:Promoter Regions, Genetic; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D000198:Spectinomycin / Q000494:pharmacology; D014170:Transformation, Genetic
chemical_list: D020121:5' Untranslated Regions; D000900:Anti-Bacterial Agents; D012336:RNA, Ribosomal, 16S; D000198:Spectinomycin; D009713:Nucleotidyltransferases; C020565:gentamicin 2''-nucleotidyltransferase; D019753:Intramolecular Lyases; C070925:lycopene cyclase-isomerase