title: ENA-A actimineral resource A extends lifespan associated with antioxidant mechanism in SMP30 knockout mice.
abstract: ENA-actimineral resource A (ENA-A) is an alkaline mineral water and has a few biological activities such as antioxidant activity. The aim of this study was to examine the effects of ENA-A on lifespan in mice using senescence marker protein-30 knockout mice. The present study had groups of 18-week-old mice (n = 24), 26-week-old mice (n = 12), and 46-week-old mice (n = 20). Each differently aged mice group was divided into three subgroups: a control group, a 5 % ENA-A-treated group, and a 10 % ENA-A-treated group. Mice in the 18-week-old group were treated with vitamin C drinking water 1.5 g/L. However, the mice in the 26-week-old and 46-week-old groups were not treated with vitamin C. The experiments were done for 18 weeks. All vitamin C-treated mice were alive at week 18 (100% survival rate). In the non-vitamin C group, the 10% ENA-A-treated mice were alive at week 18. The control and 5% ENA-A-treated mice died by week 15. As expected, vitamin C was not detected in the non-vitamin C-treated group. However, vitamin C levels were increased in an ENA-A dose-dependent manner in the vitamin C-treated group. In the TUNEL assay, a number of positive hepatocytes significantly decreased in an ENA-A dose-dependent manner. Periodic acid Schiff positive hepatocytes were significantly increased in an ENA-A dose-dependent manner. In addition, the expression level of CuZnSOD was increased by the ENA-A treatment. These data suggest that the intake of ENA-A has a critical role in the anti-aging mechanism and could be applied toward the lifespans of humans.
authors.affiliation: Department of Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu, 702-701, Republic of Korea.
authors.forename: Jung-Youn
authors.initials: JY
authors.lastname: Han
authors.forename: Meeyul
authors.initials: M
authors.lastname: Hwang
authors.forename: Sung-Yong
authors.initials: SY
authors.lastname: Hwa
authors.forename: Jin-Kyu
authors.initials: JK
authors.lastname: Park
authors.forename: Mi-Ran
authors.initials: MR
authors.lastname: Ki
authors.forename: Il-Hwa
authors.initials: IH
authors.lastname: Hong
authors.forename: Ah-Young
authors.initials: AY
authors.lastname: Kim
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Lee
authors.forename: Eun-Joo
authors.initials: EJ
authors.lastname: Lee
authors.forename: Chang-Woo
authors.initials: CW
authors.lastname: Min
authors.forename: Kyung-Ku
authors.initials: KK
authors.lastname: Kang
authors.forename: Myeong-Mi
authors.initials: MM
authors.lastname: Lee
authors.forename: Soo-Eun
authors.initials: SE
authors.lastname: Sung
authors.forename: Kyu-Shik
authors.initials: KS
authors.lastname: Jeong
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001205:Ascorbic Acid / Q000097:blood; D001206:Ascorbic Acid Deficiency / Q000201:enzymology / Q000473:pathology; D001835:Body Weight / Q000187:drug effects; D001842:Bone and Bones / Q000187:drug effects / Q000473:pathology; D002135:Calcium-Binding Proteins / Q000172:deficiency* / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects / Q000473:pathology; D015151:Immunoblotting; D047908:Intracellular Signaling Peptides and Proteins / Q000172:deficiency* / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008136:Longevity / Q000187:drug effects*; D018345:Mice, Knockout; D008903:Minerals / Q000494:pharmacology*; D028321:Plant Preparations / Q000494:pharmacology*; D013194:Staining and Labeling; D013482:Superoxide Dismutase / Q000378:metabolism; D016019:Survival Analysis
chemical_list: D000975:Antioxidants; D002135:Calcium-Binding Proteins; C518516:ENA Actimineral Resource A; D047908:Intracellular Signaling Peptides and Proteins; D008903:Minerals; D028321:Plant Preparations; C513324:Rgn protein, mouse; D006003:Glycogen; D013482:Superoxide Dismutase; D001205:Ascorbic Acid