title: The effect of zipper-interacting protein kinase on high glucose-stimulated human aortic smooth muscle cells.
abstract: Biologic abnormalities in vascular smooth muscle cells (VSMC) may perform a crucial role in the pathogenesis of diabetic vascular disease. The principal aim of this study was to determine the effects of zipper-interacting protein kinase (ZIPK) on human aortic smooth muscle cells (HASMCs) stimulated by high glucose (HG). To elucidate the role of ZIPK in HG-treated HASMCs, we overexpressed ZIPK by lentivirus infection and knocked down ZIPK by gene deletion using ZIPK shRNA. Flow cytometry and Cell Counting kit-8 (CCK-8) were separately used to analyze cell apoptosis and proliferation. Migratory activity was examined using transwell migration chamber assays. The results showed that ZIPK overexpression inhibited cell growth and migration, enhanced cell apoptosis, and reversed cell cycle disturbance by regulating the related proteins of cellular physiological process, such as human cell division cycle 14A phosphatase (Hcdc14A) and intercellular adhesion molecule 1 (ICAM-1). In conclusion, the results suggested that ZIPK plays a role in HG-treated HASMCs, indicating ZIPK is a potential therapeutic target for the treatment of diabetic vascular complications.
authors.affiliation: Institute of Endocrinology and Diabetology, Huashan Hospital, School of Medicine, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Zi
authors.initials: Z
authors.lastname: Ye
authors.affiliation: Institute of Endocrinology and Diabetology, Huashan Hospital, School of Medicine, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: Institute of Endocrinology and Diabetology, Huashan Hospital, School of Medicine, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: He
authors.affiliation: Department of Endocrinology, City University of Macau, Macau 000853, P.R. China.
authors.forename: Mancheong
authors.initials: M
authors.lastname: Leong
authors.affiliation: Institute of Endocrinology and Diabetology, Huashan Hospital, School of Medicine, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Renming
authors.initials: R
authors.lastname: Hu
authors.affiliation: Institute of Endocrinology and Diabetology, Huashan Hospital, School of Medicine, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Endocrinology and Diabetology, Huashan Hospital, School of Medicine, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Zhang
mesh_terms: D001011:Aorta / Q000166:cytology*; D002478:Cells, Cultured; D064550:Death-Associated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000201:enzymology*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D064550:Death-Associated Protein Kinases; D005947:Glucose