title: Protein covalently conjugated SU-8 surface for the enhancement of mesenchymal stem cell adhesion and proliferation.
abstract: Cell growing behavior is significantly dependent on the surface chemistry of materials. SU-8 as an epoxy-based negative photoresist is commonly used for fabricating patterned layers in lab-on-a-chip devices. As a hydrophobic material, SU-8 substrate is not favorable for cell culture, and cell attachment on native SU-8 is limited attributed to poor surface biocompatibility. Although physical adsorption of proteins could enhance the cell adhesion, the effect is not durable. In this work, SU-8 surface chemistry is modified by immobilizing fibronectin (FN) and collagen type I (COL I) covalently using (3-aminopropyl)triethoxysilane (APTES) and cross-linker glutaraldehyde (GA) to increase surface biofunctionality. The effectiveness of this surface treatment to improve the adhesion and viability of mesenchymal stem cells (MSCs) is investigated. It is found that the wettability of SU-8 surface can be significantly increased by this chemical modification. In addition, the spreading area of MSCs increases on the SU-8 surfaces with covalently conjugated matrix proteins, as compared to other unmodified SU-8 surface or those coated with proteins simply by physical adsorption. Furthermore, cell proliferation is dramatically enhanced on the SU-8 surfaces modified under the proposed scheme. Therefore, SU-8 surface modification with covalently bound matrix proteins assisted by APTES+GA provides a highly biocompatible interface for the enhanced adhesion, spreading, and proliferation of MSCs.
authors.affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University , 62 Nanyang Drive, 637459 Singapore.
authors.forename: Peng
authors.initials: P
authors.lastname: Xue
authors.forename: Jingnan
authors.initials: J
authors.lastname: Bao
authors.forename: Yon Jin
authors.initials: YJ
authors.lastname: Chuah
authors.forename: Nishanth V
authors.initials: NV
authors.lastname: Menon
authors.forename: Yilei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yuejun
authors.initials: Y
authors.lastname: Kang
mesh_terms: D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D024042:Collagen Type I / Q000737:chemistry*; D004852:Epoxy Compounds / Q000737:chemistry* / Q000494:pharmacology*; D005353:Fibronectins / Q000737:chemistry*; D005976:Glutaral / Q000737:chemistry; D055767:Immobilized Proteins / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects*; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology*; D011437:Propylamines; D012821:Silanes / Q000737:chemistry; D017155:Wettability
chemical_list: D001672:Biocompatible Materials; D024042:Collagen Type I; D004852:Epoxy Compounds; D005353:Fibronectins; D055767:Immobilized Proteins; D011108:Polymers; D011437:Propylamines; C520895:SU-8 compound; D012821:Silanes; C477625:amino-propyl-triethoxysilane; D005976:Glutaral