title: Polyphenols from olive mill waste affect biofilm formation and motility in Escherichia coli K-12.
abstract: Olive mill wastes are sources of phenolic compounds with a wide array of biological activities, including antimicrobial effects. A potential option for bioremediation to overcome ecological problems is the reutilization of these natural compounds in food production. The aim of this work was to gain a better understanding of the antimicrobial mode of action of a phenols extract from olive vegetation water (PEOVW) at molecular level by studying Escherichia coli as a model microorganism. Genome-wide transcriptional analysis was performed on E. coli K-12 exposed to PEOVW. The repression of genes for flagellar synthesis and the involvement of genes linked to biofilm formation and stress response were observed. Sub-inhibitory concentrations of PEOVW significantly decreased biofilm formation, swarming and swimming motility, thus confirming the gene expression data. This study provides interesting insights on the molecular action of PEOVW on E. coli K-12. Given these anti-biofilm properties and considering that biofilm formation is a serious problem for the food industry and human health, PEOVW has proved to be a high-value natural product.
authors.affiliation: Department of Comparative Biomedicine and Food Science, University of Padova, Legnaro, 35020, Italy.
authors.forename: Lisa
authors.initials: L
authors.lastname: Carraro
authors.forename: Luca
authors.initials: L
authors.lastname: Fasolato
authors.forename: Filomena
authors.initials: F
authors.lastname: Montemurro
authors.forename: Maria Elena
authors.initials: ME
authors.lastname: Martino
authors.forename: Stefania
authors.initials: S
authors.lastname: Balzan
authors.forename: Maurizio
authors.initials: M
authors.lastname: Servili
authors.forename: Enrico
authors.initials: E
authors.lastname: Novelli
authors.forename: Barbara
authors.initials: B
authors.lastname: Cardazzo
mesh_terms: D000890:Anti-Infective Agents / Q000378:metabolism; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D048168:Escherichia coli K12 / Q000187:drug effects* / Q000502:physiology; D020869:Gene Expression Profiling; D008124:Locomotion / Q000187:drug effects*; D031658:Olea / Q000737:chemistry*; D059808:Polyphenols / Q000378:metabolism*
chemical_list: D000890:Anti-Infective Agents; D059808:Polyphenols