title: The effect of physical and chemical cues on hepatocellular function and morphology.
abstract: Physical topographical features and/or chemical stimuli to the extracellular matrix (ECM) provide essential cues that manipulate cell functions. From the physical point of view, contoured nanostructures are very important for cell behavior in general, and for cellular functions. From the chemical point of view, ECM proteins containing an RGD sequence are known to alter cell functions. In this study, the influence of integrated physical and chemical cues on a liver cell line (HepG2) was investigated. To mimic the physical cues provided by the ECM, amorphous TiO2 nanogratings with specific dimensional and geometrical characteristics (nanogratings 90 nm wide and 150 nm apart) were fabricated. To mimic the chemical cues provided by the ECM, the TiO2 inorganic film was modified by immobilization of the RGD motif. The hepatic cell line morphological and functional changes induced by simultaneously combining these diversified cues were investigated, including cellular alignment and the expression of different functional proteins. The combination of nanopatterns and surface modification with RGD induced cellular alignment and expression of functional proteins, indicating that physical and chemical cues are important factors for optimizing hepatocyte function.
authors.affiliation: Cell-Materials Interaction Group, Biomaterials Unit, Nano-Life Field, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan. abdelaleem.shimaa@nims.go.jp.
authors.forename: Shimaa A
authors.initials: SA
authors.lastname: Abdellatef
authors.affiliation: MANA Foundry, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan. ohi.akihiko@nims.go.jp.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Ohi
authors.affiliation: MANA Foundry, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan. nabatame.toshihide@nims.go.jp.
authors.forename: Toshihide
authors.initials: T
authors.lastname: Nabatame
authors.affiliation: Cell-Materials Interaction Group, Biomaterials Unit, Nano-Life Field, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan. taniguchi.akiyoshi@nims.go.jp.
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Taniguchi
mesh_terms: D000418:Albumins / Q000737:chemistry; D006528:Carcinoma, Hepatocellular / Q000737:chemistry / Q000473:pathology / Q000503:physiopathology; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry; D005109:Extracellular Matrix / Q000737:chemistry*; D016326:Extracellular Matrix Proteins / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D022001:Focal Adhesions; D056945:Hep G2 Cells; D006801:Humans; D019012:Integrin beta1 / Q000737:chemistry; D008099:Liver / Q000737:chemistry* / Q000473:pathology / Q000503:physiopathology; D008113:Liver Neoplasms / Q000737:chemistry / Q000473:pathology / Q000503:physiopathology; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D014168:Transferrin / Q000737:chemistry
chemical_list: D000418:Albumins; D016326:Extracellular Matrix Proteins; D019012:Integrin beta1; D009842:Oligopeptides; D014168:Transferrin; C009495:titanium dioxide; C047981:arginyl-glycyl-aspartic acid; D003577:Cytochrome P-450 Enzyme System; D014025:Titanium