title: Biofilm formation of salivary microbiota on dental restorative materials analyzed by denaturing gradient gel electrophoresis and sequencing.
abstract: The microbial diversity of biofilms formed on the surfaces of amalgam, glass-ionomer cement, and resin composite was analyzed by denaturing gradient gel electrophoresis (DGGE). The V2-V3 region of salivary microbial 16S rDNA gene sequences of planktonic and biofilm bacteria, after 1 day and 1 week of incubation, was amplified by polymerase chain reaction (PCR) and analyzed by DGGE. The amounts of strongly adherent phylotypes after 1 day and 1 week on the three dental restorative materials were more than those on hydroxyapatite. Streptococcus salivarius was detected in both loosely adherent and strong adherent groups of all 1-day samples. At 1 week, the amounts of loosely adherent and strongly adherent phylotypes present on the three restorative materials ranked in this ascending order: glass-ionomer cement < resin composite < amalgam. Results of DGGE analysis suggested that glass-ionomer cement was the best material of choice in terms of suppressing bacterial phylotypes in biofilms.
authors.affiliation: Comprehensive Dental Care (Endodontics), Faculty of Dentistry, The University of Hong Kong.
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.forename: Lihong
authors.initials: L
authors.lastname: Guo
authors.forename: Chaminda Jayampath
authors.initials: CJ
authors.lastname: Seneviratne
authors.forename: Bo
authors.initials: B
authors.lastname: Huang
authors.forename: Jianmin
authors.initials: J
authors.lastname: Han
authors.forename: Lei
authors.initials: L
authors.lastname: Peng
authors.forename: Xiaodi
authors.initials: X
authors.lastname: Liu
authors.forename: Chengfei
authors.initials: C
authors.lastname: Zhang
mesh_terms: D018441:Biofilms; D003793:Dental Restoration, Permanent; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D064307:Microbiota; D012463:Saliva / Q000382:microbiology*