title: The efficacy of the well of the well (WOW) culture system on development of bovine embryos in a small group and the effect of number of adjacent embryos on their development.
abstract: The aim of the present study was to clarify the efficacy of the well of the well (WOW) culture system for a small number of embryos and the effect of number of adjacent embryos in a WOW dish on blastocyst development. In conventional droplet culture, embryos in the small-number group (5-6 embryos/droplet) showed low blastocyst development compared with a control group (25-26 embryos/droplet). However, small and large numbers of embryos (5-6 and 25 embryos, respectively) in a WOW dish showed no significant differences in cleavage, blastocyst rates, and mean cell number in blastocysts compared with the control group (25-30 embryos/droplet). In addition, the number of adjacent embryos in a WOW dish did not affect the development to blastocysts and cell number in blastocysts. In conclusion, a WOW dish can provide high and stable blastocyst development in small group culture wherever embryos are placed in microwells of the WOW dish.
authors.affiliation: Institute: Laboratory of Theriogenology,Department of Veterinary Clinical Sciences,Graduate School of Veterinary Medicine,Hokkaido University,Sapporo 060-0818,Japan.
authors.forename: Sung-Sik
authors.initials: SS
authors.lastname: Kang
authors.affiliation: Institute: Laboratory of Theriogenology,Department of Veterinary Clinical Sciences,Graduate School of Veterinary Medicine,Hokkaido University,Sapporo 060-0818,Japan.
authors.forename: Sosuke
authors.initials: S
authors.lastname: Ofuji
authors.affiliation: National Livestock Breeding Center,Nishigo,Fukushima 961-8511,Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Imai
authors.affiliation: Institute: Laboratory of Theriogenology,Department of Veterinary Clinical Sciences,Graduate School of Veterinary Medicine,Hokkaido University,Sapporo 060-0818,Japan.
authors.forename: Weiping
authors.initials: W
authors.lastname: Huang
authors.affiliation: Institute: Laboratory of Theriogenology,Department of Veterinary Clinical Sciences,Graduate School of Veterinary Medicine,Hokkaido University,Sapporo 060-0818,Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Koyama
authors.affiliation: Institute: Laboratory of Theriogenology,Department of Veterinary Clinical Sciences,Graduate School of Veterinary Medicine,Hokkaido University,Sapporo 060-0818,Japan.
authors.forename: Yojiro
authors.initials: Y
authors.lastname: Yanagawa
authors.affiliation: Institute: Laboratory of Theriogenology,Department of Veterinary Clinical Sciences,Graduate School of Veterinary Medicine,Hokkaido University,Sapporo 060-0818,Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Takahashi
authors.affiliation: Laboratory of Theriogenology,Department of Veterinary Clinical Sciences,Graduate School of Veterinary Medicine,Hokkaido University,Kita-ku Kita 18 Nishi 9,Sapporo 060-0818,Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Nagano
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000502:physiology*; D002417:Cattle; D046149:Embryo Culture Techniques / Q000295:instrumentation / Q000379:methods*; D005260:Female; D005307:Fertilization in Vitro; D008297:Male
keywords: WOW