title: Determination of fat and total protein content in milk using conventional digital imaging.
abstract: The applicability of conventional digital imaging to quantitative determination of fat and total protein in cow's milk, based on the phenomenon of light scatter, has been proved. A new algorithm for extracting features from digital images of milk samples has been developed. The algorithm takes into account spatial distribution of light, diffusely transmitted through a sample. The proposed method has been tested on two sample sets prepared from industrial raw milk standards, with variable fat and protein content. Partial Least-Squares (PLS) regression on the features calculated from images of monochromatically illuminated milk samples resulted in models with high prediction performance when analysed the sets separately (best models with cross-validated R(2)=0.974 for protein and R(2)=0.973 for fat content). However when analysed the sets jointly with the obtained results were significantly worse (best models with cross-validated R(2)=0.890 for fat content and R(2)=0.720 for protein content). The results have been compared with previously published Vis/SW-NIR spectroscopic study of similar samples.
authors.affiliation: Section of Chemical Engineering, Aalborg University, Campus Esbjerg, Niels Bohrs vej, 8, Esbjerg 6700, Denmark. Electronic address: svk@bio.aau.dk.
authors.forename: Sergey
authors.initials: S
authors.lastname: Kucheryavskiy
authors.affiliation: Samara State Technical University, Samara, Russia.
authors.forename: Anastasiia
authors.initials: A
authors.lastname: Melenteva
authors.affiliation: Samara State Technical University, Samara, Russia; J&M Analytik AG, Essingen, Germany.
authors.forename: Andrey
authors.initials: A
authors.lastname: Bogomolov
mesh_terms: D000465:Algorithms; D000818:Animals; D005223:Fats / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000032:analysis*
chemical_list: D005223:Fats; D008894:Milk Proteins
keywords: Digital imaging; Image analysis; Light scatter; Milk quality