title: Preparation of peptide microspheres using tumor antigen-derived peptides.
abstract: Due to its distinct biological attributes, poly(D,L lactide-co glycolide) (PLGA) is one of the most preferred methods for DNA/protein/peptide encapsulation for therapeutics. Importantly, PLGA acts as an adjuvant for weakly immunogenic antigens and mimics booster responses after a single dose of administration, thereby serving as a single-shot vaccine delivery vehicle. Efficient delivery of antigens to antigen-presenting cells (APC) has been made possible by the use of a PLGA particle-based vaccine delivery system. Also, the plasma half-life of the PLGA-encapsulated vaccine increases as it is protected from degradation, prior to its further release. PLGAs are reported to be catabolized into individual nontoxic units once inside the host and further degraded via normal metabolic pathways. In this chapter, we have described the preparation and characterization of tumor peptide encapsulated PLGA microparticles as a model for controlled-release peptide delivery system.
authors.affiliation: Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Santwana
authors.initials: S
authors.lastname: Bhatnagar
authors.forename: Raza Ali
authors.initials: RA
authors.lastname: Naqvi
authors.forename: Riyasat
authors.initials: R
authors.lastname: Ali
authors.forename: D N
authors.initials: DN
authors.lastname: Rao
mesh_terms: D000951:Antigens, Neoplasm / Q000008:administration & dosage / Q000737:chemistry* / Q000276:immunology; D002470:Cell Survival / Q000187:drug effects; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry* / Q000633:toxicity; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010316:Particle Size; D010455:Peptides / Q000008:administration & dosage / Q000737:chemistry* / Q000276:immunology; D011100:Polyglycolic Acid / Q000737:chemistry* / Q000633:toxicity; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer
chemical_list: D000951:Antigens, Neoplasm; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D010455:Peptides; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid