title: Location of novel benzanthrone dyes in model membranes as revealed by resonance energy transfer.
abstract: Förster resonance energy transfer (FRET) between anthrylvinyl-labeled phosphatidylcholine (AV-PC) as a donor and newly synthesized benzanthrones (referred to here as A8, A6, AM12, AM15 and AM18) as acceptors has been examined to gain insight into molecular level details of the interactions between benzanthrone dyes and model lipid membranes composed of zwitterionic lipid phosphatidylcholine and its mixtures with anionic lipids cardiolipin (CL) and phosphatidylglycerol (PG). FRET data were quantitatively analyzed in terms of the model of energy transfer in two-dimensional systems taking into account the distance dependence of orientation factor. Evidence for A8 location in phospholipid headgroup region has been obtained. Inclusion of CL and PG into PC bilayer has been found to induce substantial relocation of A6, AM12, AM15 and AM18 from hydrophobic membrane core to lipid-water interface.
authors.affiliation: Department of Nuclear and Medical Physics, V.N. Karazin Kharkiv National University, 4 Svobody Sq., Kharkiv, 61077, Ukraine, olya_zhitniakivska@yahoo.com.
authors.forename: Olga
authors.initials: O
authors.lastname: Zhytniakivska
authors.forename: Valeriya
authors.initials: V
authors.lastname: Trusova
authors.forename: Galyna
authors.initials: G
authors.lastname: Gorbenko
authors.forename: Elena
authors.initials: E
authors.lastname: Kirilova
authors.forename: Inta
authors.initials: I
authors.lastname: Kalnina
authors.forename: Georgiy
authors.initials: G
authors.lastname: Kirilov
authors.forename: Julian
authors.initials: J
authors.lastname: Molotkovsky
authors.forename: Jukka
authors.initials: J
authors.lastname: Tulkki
authors.forename: Paavo
authors.initials: P
authors.lastname: Kinnunen
mesh_terms: D001551:Benz(a)Anthracenes / Q000737:chemistry*; D002308:Cardiolipins / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008962:Models, Theoretical; D010713:Phosphatidylcholines / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D001551:Benz(a)Anthracenes; D002308:Cardiolipins; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; C012768:benzanthrone