title: [A rapid dialysis method for analysis of artificial sweeteners in food].
abstract: A simple and rapid dialysis method was developed for the extraction and purification of four artificial sweeteners, namely, sodium saccharin (Sa), acesulfame potassium (AK), aspartame (APM), and dulcin (Du), which are present in various foods. Conventional dialysis uses a membrane dialysis tube approximately 15 cm in length and is carried out over many hours owing to the small membrane area and owing to inefficient mixing. In particular, processed cereal products such as cookies required treatment for 48 hours to obtain satisfactory recovery of the compounds. By increasing the tube length to 55 cm and introducing efficient mixing by inversion at half-hour intervals, the dialysis times of the four artificial sweeteners, spiked at 0.1 g/kg in the cookie, were shortened to 4 hours. Recovery yields of 88.9-103.2% were obtained by using the improved method, whereas recovery yields were low (65.5-82.0%) by the conventional method. Recovery yields (%) of Sa, AK, APM, and Du, spiked at 0.1 g/kg in various foods, were 91.6-100.1, 93.9-100.1, 86.7-100.0 and 88.7-104.7 using the improved method.
authors.affiliation: Tokyo Metropolitan Institute of Public Health.
authors.forename: Shoichi
authors.initials: S
authors.lastname: Tahara
authors.forename: Takushi
authors.initials: T
authors.lastname: Fujiwara
authors.forename: Akiko
authors.initials: A
authors.lastname: Yasui
authors.forename: Chieko
authors.initials: C
authors.lastname: Hayafuji
authors.forename: Chigusa
authors.initials: C
authors.lastname: Kobayashi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Uematsu
mesh_terms: D001218:Aspartame / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003956:Dialysis / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D010671:Phenylurea Compounds / Q000302:isolation & purification*; D012439:Saccharin / Q000302:isolation & purification*; D013549:Sweetening Agents / Q000302:isolation & purification*; D013843:Thiazines / Q000302:isolation & purification*; D013997:Time Factors
chemical_list: D010671:Phenylurea Compounds; D013549:Sweetening Agents; D013843:Thiazines; C004352:dulcin; D012439:Saccharin; C006362:acetosulfame; D001218:Aspartame