title: [Differentiation of in vitro induced human pluripotent stem cells into hematopoietic stem/progenitor cells].
abstract: This study was aimed to explore the differentiation of in vitro induced human pluripotent stem cells (iPSC) into hematopoietic stem progenitor cells. The human iPSC were induced to differentiate into hematopoietic stem/progenitor cell by co-culturing with OP9 bone marrow stromal cells. The expression of hematopoietic stem/progenitor cell surface markers were detected by flow cytometry. The regulation gene expressions of iPSC and hematopoietic stem/progenitor cells were measured by real-time PCR. The CD34(+) hematopoietic stem/progenitor cells were isolated by using immunomagnetic beads, and were used for colony formation assay. The results showed that after iPSC were co-cultured with OP9 cells for 4 days, the morphological changes of iPSC could be observed. Hematopoietic stem/progenitor cell surface markers CD34 and CD43 could be detected by flow cytometry after differentiation. The pluripotent marker gene OCT4 expression gradually decreased and blood-related transcription factor Gata-2 expression gradually increased, while Runx-1 expression was wavily changed, CD34 expression gradually increased. The erythroid colony(CFU-E), granulocyte colony(CFU-G), megakaryocytic colony(CFU-M), granulocyte-megakaryocytic colony(CFU-GM), and mixed colony(CFU-GEMM) were obtained after cultures for 14 d. It is concluded that the human iPSC cells can be induced to differentiate into hematopoietic stem/progenitor cells in vitro by co-culture with OP9 cells.
authors.affiliation: Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan 250062, Shandong Province, China; School of Medicine and Life Sciences, Jinan University, Jinan 250022, Shandong Province, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510602, Guangdong Province, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Huang
authors.affiliation: Cryomedicine Laboratory, Qilu Hospital, Shandong University, Jinan 250012, Shandong Province, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.affiliation: Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510602, Guangdong Province, China.
authors.forename: Guang-Jin
authors.initials: GJ
authors.lastname: Pan
authors.affiliation: Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan 250062, Shandong Province, China; School of Medicine and Life Sciences, Jinan University, Jinan 250022, Shandong Province, China.
authors.forename: Yun-Shan
authors.initials: YS
authors.lastname: Wang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D051379:Mice