title: Analysis of nitric oxide synthase gene polymorphisms in neonatal respiratory distress syndrome among the Chinese Han population.
abstract: OBJECTIVE
  To evaluate the association of NOS1 and NOS3 gene polymorphisms with the risk/severity of neonatal respiratory distress syndrome (RDS) among preterm infants.
  
  
  METHODS
  The patient group was 189 preterm infants diagnosed with RDS. The control group was 227 preterm neonates who did not develop RDS. NOS genotyping was performed using an improved multiplex ligation detection reaction (iMLDR) technique based on LDR.
  
  
  RESULTS
  It was found that genotype and allele frequencies of rs2682826 of the NOS1 gene and rs1799983 of the NOS3 gene were not significantly different between the RDS group and the control group. However, when the preterm infants were divided into two and three groups based on gestational age and birth weight, a study of the SNP rs1799983 of the NOS3 gene showed that the GG genotype and G allele frequencies were significantly increased in the RDS groups, the GT genotype and A allele were less frequent among the RDS groups in 26-32.9 weeks of gestational age and in a birth weight subgroup of <1.5 Kg.
  
  
  CONCLUSIONS
  Our study raises the possibility that a genetic variation of NOS3 could be implicated in the pathophysiology of RDS in the Chinese Han population, especially in very preterm and very low birth weight infants.
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
authors.forename: Jiang
authors.initials: J
authors.lastname: Du
authors.affiliation: Center of pediatrics, Zhujiang Hospital, Southern Medical university, Guangzhou, Guangdong 510282, China. wangbin6556@126.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Qiyi
authors.initials: Q
authors.lastname: Zeng
mesh_terms: D000328:Adult; D000483:Alleles; D002681:China / Q000453:epidemiology; D004247:DNA / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D005865:Gestational Age; D006801:Humans; D015994:Incidence; D007231:Infant, Newborn; D007234:Infant, Premature; D007235:Infant, Premature, Diseases / Q000201:enzymology / Q000453:epidemiology / Q000235:genetics*; D008297:Male; D019001:Nitric Oxide Synthase / Q000235:genetics* / Q000378:metabolism; D052248:Nitric Oxide Synthase Type I / Q000235:genetics / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012127:Respiratory Distress Syndrome, Newborn / Q000201:enzymology / Q000453:epidemiology / Q000235:genetics*
chemical_list: D004247:DNA; C496320:NOS1 protein, human; C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; D052250:Nitric Oxide Synthase Type III