title: Influence of chromatin condensation on the number of direct DSB damages induced by ions studied using a Monte Carlo code.
abstract: The purpose of this work is to evaluate the influence of the chromatin condensation on the number of direct double-strand break (DSB) damages induced by ions. Two geometries of chromosome territories containing either condensed or decondensed chromatin were implemented as biological targets in the Geant4 Monte Carlo simulation code and proton and alpha irradiation was simulated using the Geant4-DNA processes. A DBSCAN algorithm was used in order to detect energy deposition clusters that could give rise to single-strand breaks or DSBs on the DNA molecule. The results of this study show an increase in the number and complexity of DNA DSBs in condensed chromatin when compared with decondensed chromatin.
authors.affiliation: Institut de Radioprotection et de Sureté Nucléaire, IRSN, BP17, Fontenay aux roses 92962, France.
authors.forename: M
authors.initials: M
authors.lastname: Dos Santos
authors.affiliation: Institut de Radioprotection et de Sureté Nucléaire, IRSN, BP17, Fontenay aux roses 92962, France.
authors.forename: I
authors.initials: I
authors.lastname: Clairand
authors.affiliation: Institut de Radioprotection et de Sureté Nucléaire, IRSN, BP17, Fontenay aux roses 92962, France.
authors.forename: G
authors.initials: G
authors.lastname: Gruel
authors.affiliation: Institut de Radioprotection et de Sureté Nucléaire, IRSN, BP17, Fontenay aux roses 92962, France.
authors.forename: J F
authors.initials: JF
authors.lastname: Barquinero
authors.affiliation: Centre d'Etudes Nucléaires de Bordeaux Gradignan, CENBG, Université de Bordeaux 1, CNRS/IN2P3, chemin du solarium, BP 120, Gradignan 33175, France.
authors.forename: S
authors.initials: S
authors.lastname: Incerti
authors.affiliation: Institut de Radioprotection et de Sureté Nucléaire, IRSN, BP17, Fontenay aux roses 92962, France carmen.villagrasa@irsn.fr.
authors.forename: C
authors.initials: C
authors.lastname: Villagrasa
mesh_terms: D000465:Algorithms; D000512:Alpha Particles; D000818:Animals; D002843:Chromatin / Q000737:chemistry*; D002875:Chromosomes / Q000648:ultrastructure; D016000:Cluster Analysis; D003198:Computer Simulation; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D053903:DNA Breaks, Double-Stranded; D004249:DNA Damage; D004583:Electrons; D006801:Humans; D007477:Ions; D018499:Linear Energy Transfer; D009010:Monte Carlo Method; D009707:Nucleosomes / Q000737:chemistry; D011522:Protons; D012984:Software
chemical_list: D002843:Chromatin; D007477:Ions; D009707:Nucleosomes; D011522:Protons; D004247:DNA