title: Effects of selenium on liver and muscle contents and urinary excretion of zinc, copper, iron and manganese.
abstract: Selenium is a main component of glutathione peroxidase (GPX), a key antioxidant enzyme. Other elements, such as zinc, copper, manganese and iron, are also involved in the pathogenesis of oxidative damage as well as in other important metabolic pathways. The effects of selenium supplementation on the metabolism of these elements have yield controversial results .The aim of this study is to analyse the effects of selenium supplementation on liver, muscle and urinary excretion of zinc, copper, iron and manganese in a situation of oxidative stress, such as protein deficiency. The experimental design included four groups of adult male Sprague-Dawley rats, which received the Lieber-DeCarli control diet, an isocaloric 2 % protein-containing diet and another similar two groups to which selenomethionine (6 mg/l liquid diet) was added. After sacrifice (5 weeks later), muscle, liver and serum selenium were determined, as well as muscle, liver and urinary zinc, copper, manganese and iron and liver GPX activity and liver malondialdehyde. Selenium addition led to decreased liver copper, increased muscle copper, increased copper excretion and increased liver iron, whereas zinc and manganese parameters were essentially unaltered. Muscle, liver and serum selenium were all significantly correlated with liver GPX activity.
authors.affiliation: Servicio de Medicina Interna, Hospital Universitario de Canarias, Tenerife, Canary Islands, Spain.
authors.forename: María José
authors.initials: MJ
authors.lastname: Monedero-Prieto
authors.forename: José María
authors.initials: JM
authors.lastname: González-Pérez
authors.forename: Emilio
authors.initials: E
authors.lastname: González-Reimers
authors.forename: Onán
authors.initials: O
authors.lastname: Hernández-Pérez
authors.forename: María
authors.initials: M
authors.lastname: Monereo-Muñoz
authors.forename: Luis
authors.initials: L
authors.lastname: Galindo-Martín
authors.forename: Geraldine
authors.initials: G
authors.lastname: Quintero-Platt
authors.forename: Pedro
authors.initials: P
authors.lastname: Abreu-González
mesh_terms: D000818:Animals; D003300:Copper / Q000652:urine*; D007501:Iron / Q000652:urine*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008345:Manganese / Q000652:urine*; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000494:pharmacology*; D015032:Zinc / Q000652:urine*
chemical_list: D008345:Manganese; D003300:Copper; D007501:Iron; D012643:Selenium; D015032:Zinc