title: Plastid transformation in lettuce (Lactuca sativa L.) by biolistic DNA delivery.
abstract: The interest in producing pharmaceutical proteins in a nontoxic plant host has led to the development of an approach to express such proteins in transplastomic lettuce (Lactuca sativa L.). A number of therapeutic proteins and vaccine antigen candidates have been stably integrated into the lettuce plastid genome using biolistic DNA delivery. High levels of accumulation and retention of biological activity suggest that lettuce may provide an ideal platform for the production of biopharmaceuticals.
authors.affiliation: Integrative Biology, University of Texas at Austin, Austin, TX, USA.
authors.forename: Tracey A
authors.initials: TA
authors.lastname: Ruhlman
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D019470:Biolistics / Q000379:methods*; D002478:Cells, Cultured; D021901:DNA, Intergenic / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D018545:Lactuca / Q000201:enzymology / Q000235:genetics*; D009713:Nucleotidyltransferases / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics; D018087:Plastids / Q000235:genetics*; D011523:Protoplasts / Q000166:cytology; D000198:Spectinomycin / Q000494:pharmacology; D019076:Transgenes / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D021901:DNA, Intergenic; D000198:Spectinomycin; D009713:Nucleotidyltransferases; C020565:gentamicin 2''-nucleotidyltransferase