title: A metabonomic analysis of serum from rats treated with ricinine using ultra performance liquid chromatography coupled with mass spectrometry.
abstract: A metabonomic approach based on ultra performance liquid chromatography coupled with mass spectrometry (UPLC/MS) was used to study the hepatotoxicity of ricinine in rats. Potential biomarkers of ricinine toxicity and toxicological mechanism were analyzed by serum metabonomic method. The significant differences in the metabolic profiling of the control and treated rats were clear by using the principal components analysis (PCA) of the chromatographic data. Significant changes of metabolite biomarkers like phenylalanine, tryptophan, cholic acid, LPC and PC were detected in the serum. These biochemical changes were related to the metabolic disorders in amino acids and phospholipids. This research indicates that UPLC/MS-based metabonomic analysis of serum samples can be used to predict the hepatotoxicity and further understand the toxicological mechanism induced by ricinine. This work shows that metabonomics method is a valuable tool in drug mechanism study.
authors.affiliation: School of Pharmacy, China Medical University, Shenyang, Liaoning, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Peng
authors.affiliation: School of Pharmacy, China Medical University, Shenyang, Liaoning, China; Department of Pharmacy, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Cai
authors.affiliation: School of Pharmacy, China Medical University, Shenyang, Liaoning, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Pharmacy, China Medical University, Shenyang, Liaoning, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhao
authors.affiliation: School of Pharmacy, China Medical University, Shenyang, Liaoning, China.
authors.forename: Ting-jian
authors.initials: TJ
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, China Medical University, Shenyang, Liaoning, China.
authors.forename: Zai-xing
authors.initials: ZX
authors.lastname: Chen
authors.affiliation: School of Pharmacy, China Medical University, Shenyang, Liaoning, China.
authors.forename: Fan-hao
authors.initials: FH
authors.lastname: Meng
mesh_terms: D000470:Alkaloids / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D001774:Blood Chemical Analysis; D002853:Chromatography, Liquid; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D013058:Mass Spectrometry; D055442:Metabolome / Q000187:drug effects*; D055432:Metabolomics / Q000379:methods; D019906:Nuclear Magnetic Resonance, Biomolecular; D011728:Pyridones / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats
chemical_list: D000470:Alkaloids; D015415:Biomarkers; D004365:Drugs, Chinese Herbal; D011728:Pyridones; C019550:ricinine