title: [The role of immunodeficiencies in development of complications during vaccination of children with BCG vaccine].
abstract: OBJECTIVE
  Study of the interrelation between the presence of immune deficiency and development of complications during vaccination of newborns with BCG vaccine.
  
  
  METHODS
  In 24 children with complications of vaccine process in the form of cold abscess and lymphadenitis indicators of lymphocyte subpopulation levels were studied by flow cytofluorimetry on Beckman Coulter cytofluoriemter by using monoclonal antibodies with markers CD45+CD3+ - T-cell, CD45+CD3+CD4+ - T-helpers, CD45+CD3+CD8+ - T-supressors-cytotoxic killers, CD45+CD3 CD16+CD56+ - natural killers, CD45+CD3-CD19+ - B-lymphocytes. The level of IgG, IgA, IgM in sera was determined by immune diffusion method in agar by Mancini.
  
  
  RESULTS
  In 4 children selective deficiency of IgA, in 5 - hyper-IgM syndrome was detected, which is an innate immunodeficiency and is characterized by the lack of sera IgA, reduction of IgG level and increase of IgM. In 9 children a reduction of CD16+ natural killer lymphocytes was detected, in some cases combined with a reduction of CD8+ T-supressors-cytotoxic killers.
  
  
  CONCLUSIONS
  The reason of development of complications during BCG administration is the presence of immunodeficiency in children. In these children severe course of the vaccine process, presence of axillary lymphadenitis was observed, therapy of these children continued from 4 to 6 months.
authors.forename: L I
authors.initials: LI
authors.lastname: Krasnoproshina
authors.forename: T A
authors.initials: TA
authors.lastname: Sevast'ianova
authors.forename: V A
authors.initials: VA
authors.lastname: Aksenova
authors.forename: T A
authors.initials: TA
authors.lastname: Serova
authors.forename: I V
authors.initials: IV
authors.lastname: Bisheva
authors.forename: S A
authors.initials: SA
authors.lastname: Skhodova
authors.forename: V V
authors.initials: VV
authors.lastname: Zverev
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000009:adverse effects*; D000906:Antibodies / Q000097:blood / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D001500:BCG Vaccine / Q000008:administration & dosage / Q000009:adverse effects*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D053306:Hyper-IgM Immunodeficiency Syndrome / Q000097:blood* / Q000139:chemically induced* / Q000276:immunology; D007223:Infant; D007694:Killer Cells, Natural / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008199:Lymphadenitis / Q000097:blood* / Q000139:chemically induced* / Q000276:immunology; D018655:Lymphocyte Count; D008297:Male; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology / Q000378:metabolism / Q000473:pathology
chemical_list: D000276:Adjuvants, Immunologic; D000906:Antibodies; D001500:BCG Vaccine