title: Continuous and simultaneous electrochemical measurements of glucose, lactate, and ascorbate in rat brain following brain ischemia.
abstract: Developing new tools and technologies to enable recording the dynamic changes of multiple neurochemicals is the essence of better understanding of the molecular basis of brain functions. This study demonstrates a microfluidic chip-based online electrochemical system (OECS) for in vivo continuous and simultaneous monitoring of glucose, lactate, and ascorbate in rat brain. To fabricate the microfluidic chip-based detecting system, a microfluidic chip with patterned channel is developed into an electrochemical flow cell by incorporating the chip with three surface-modified indium-tin oxide (ITO) electrodes as working electrodes, a Ag/AgCl wire as reference electrode, and a stainless steel tube as counter electrode. Selective detection of ascorbate is achieved by the use of single-walled carbon nanotubes (SWNTs) to largely facilitate the electrochemical oxidation of ascorbate, while a dehydrogenase-based biosensing mechanism with methylene green (MG) adsorbed onto SWNTs as an electrocatalyst for the oxidation of dihydronicotiamide adenine dinucleotide (NADH) is employed for biosensing of glucose and lactate. To avoid the crosstalk among three sensors, the sensor alignment is carefully designed with the SWNT-modified electrode in the upstream channel and paralleled glucose and lactate biosensors in the downstream channels. With the microfluidic chip-based electrochemical flow cell as the detector, an OECS is successfully established by directly integrating the microfluidic chip-based electrochemical flow cell with in vivo microdialysis. The OECS exhibits a good linear response toward glucose, lactate, and ascorbate with less crosstalk. This property, along with the high stability and selectivity, enables the OECS for continuously monitoring three species in rat brain following brain ischemia.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, The Chinese Academy of Sciences , Beijing 100190, China.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Lin
authors.forename: Ping
authors.initials: P
authors.lastname: Yu
authors.forename: Jie
authors.initials: J
authors.lastname: Hao
authors.forename: Yuexiang
authors.initials: Y
authors.lastname: Wang
authors.forename: Takeo
authors.initials: T
authors.lastname: Ohsaka
authors.forename: Lanqun
authors.initials: L
authors.lastname: Mao
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000737:chemistry* / Q000378:metabolism; D015374:Biosensing Techniques; D001923:Brain Chemistry; D002545:Brain Ischemia / Q000378:metabolism*; D004563:Electrochemistry; D004566:Electrodes; D005947:Glucose / Q000737:chemistry* / Q000378:metabolism; D007202:Indicators and Reagents; D007204:Indium; D019344:Lactic Acid / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D008838:Microcomputers; D046210:Microfluidic Analytical Techniques; D010084:Oxidation-Reduction; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D017971:Tin Compounds
chemical_list: D007202:Indicators and Reagents; D017971:Tin Compounds; D007204:Indium; D019344:Lactic Acid; C047711:indium oxide; D005947:Glucose; C045358:stannic oxide; D001205:Ascorbic Acid