title: Influence of Annona muricata (soursop) on biodistribution of radiopharmaceuticals in rats.
abstract: OBJECTIVE
  To evaluate the effect of hydroalcoholic extract of A. muricata on biodistribution of two radiopharmaceuticals: sodium phytate and dimercaptosuccinic acid (DMSA), both labeled with 99mtechnetium.
  
  
  METHODS
  Twenty four Wistar rats were divided into two treated groups and two controls groups. The controls received water and the treated received 25mg/kg/day of A. muricata by gavage for ten days. One hour after the last dose, the first treated group received 99mTc-DMSA and the second sodium 99mTc-phytate (0.66MBq each group), both via orbital plexus. Controls followed the same protocol. Forty min later, all groups were sacrificed and the blood, kidney and bladder were isolated from the first treated group and the blood, spleen and liver isolated from the second treated group. The percentage of radioactivity per gram of tissue (%ATI/g) was calculated using a gamma counter.
  
  
  RESULTS
  The statistical analysis showed that there was a statistically significant decrease (p<0.05) in the uptake of %ATI/g in bladder (0.11±0.01and1.60±0.08), kidney (3.52±0.51and11.84±1.57) and blood (0.15±0.01and 0.54±0.05) between the treated group and control group, respectively.
  
  
  CONCLUSIONS
  The A. muricata hydroalcoholic extract negatively influenced the uptake of 99mTc-DMSA in bladder, kidney and blood of rats.
authors.affiliation: Federal University of Rio Grande do Norte, Department of Microbiology and Parasitology, NatalRN, Brazil, PhD, Associate Professor, Head of Experimental Radiobiology and Antiparasitic Assays Laboratory, Department of Microbiology and Parasitology, Federal University of Rio Grande do Norte (UFRN), Natal-RN, Brazil. Technical procedures, manuscript writing.
authors.forename: Cecília Maria de Carvalho Xavier
authors.initials: CM
authors.lastname: Holanda
authors.affiliation: UFRN, School of Medicine, NatalRN, Brazil, Graduate student, School of Medicine, UFRN, Natal-RN, Brazil. Grant from Institutional program for Scientific Initiation (PIBIC) of the National Council of Technological and Scientific Development (CNPq), Ministry of Science, Techmology and Inovation, Brazil. Manuscript preparation.
authors.forename: Delianne Azevedo
authors.initials: DA
authors.lastname: Barbosa
authors.affiliation: UFRN, School of Medicine, NatalRN, Brazil, Graduate student, School of Medicine, UFRN, Natal-RN, Brazil. Grant from Institutional program for Scientific Initiation (PIBIC) of the National Council of Technological and Scientific Development (CNPq), Ministry of Science, Techmology and Inovation, Brazil. Manuscript preparation.
authors.forename: Vanessa Fávero
authors.initials: VF
authors.lastname: Demeda
authors.affiliation: UFRN, School of Medicine, NatalRN, Brazil, Graduate student, School of Medicine, UFRN, Natal-RN, Brazil. Grant from Institutional program for Scientific Initiation (PIBIC) of the National Council of Technological and Scientific Development (CNPq), Ministry of Science, Techmology and Inovation, Brazil. Manuscript preparation.
authors.forename: Flora Tamires Moura
authors.initials: FT
authors.lastname: Bandeira
authors.affiliation: UFRN, NatalRN, Brazil, Master, Postgraduate Program in Biochemical, UFRN, Natal-RN, Brazil. Technical support in radiopharmaceuticals.
authors.forename: Hilkéa Carla Souza de
authors.initials: HC
authors.lastname: Medeiros
authors.affiliation: UFRN, NatalRN, Brazil, Master, Postgraduate Program in Biochemical, UFRN, Natal-RN, Brazil. Technical support in radiopharmaceuticals.
authors.forename: Kércia Regina Santos Gomes
authors.initials: KR
authors.lastname: Pereira
authors.affiliation: Federal University of Campina Grande, Center for Education and Health, Campina GrandePB, Brazil, PhD, Associate Professor, Center for Education and Health, Federal University of Campina Grande, Campina Grande-PB, Brazil. Manuscript writing.
authors.forename: Vanessa Santos de Arruda
authors.initials: VS
authors.lastname: Barbosa
authors.affiliation: UFRN, Department of Surgery, NatalRN, Brazil, PhD, Full Professor, Head of Nucleus of Experimental Surgery, Department of Surgery, UFRN, Natal-RN, Brazil. Analysis of data, manuscript writing, critical revision.
authors.forename: Aldo Cunha
authors.initials: AC
authors.lastname: Medeiros
mesh_terms: D000818:Animals; D031041:Annona / Q000737:chemistry*; D004347:Drug Interactions; D007668:Kidney / Q000187:drug effects; D008297:Male; D010833:Phytic Acid / Q000097:blood / Q000493:pharmacokinetics*; D010936:Plant Extracts / Q000097:blood / Q000493:pharmacokinetics*; D019275:Radiopharmaceuticals / Q000097:blood / Q000493:pharmacokinetics*; D017208:Rats, Wistar; D013154:Spleen / Q000187:drug effects; D019783:Technetium Tc 99m Dimercaptosuccinic Acid / Q000097:blood / Q000493:pharmacokinetics*; D014018:Tissue Distribution; D001743:Urinary Bladder / Q000187:drug effects
chemical_list: D010936:Plant Extracts; D019275:Radiopharmaceuticals; D019783:Technetium Tc 99m Dimercaptosuccinic Acid; D010833:Phytic Acid