title: Changes in IgG and total plasma protein glycomes in acute systemic inflammation.
abstract: Recovery after cardiac surgery is a complex process that has to compensate for both individual variability and extensive tissue damage in the context of systemic inflammation. Protein glycosylation is essential in many steps of the inflammatory cascade, but due to technological limitations the role of individual variation in glycosylation in systemic inflammation has not been addressed until now. We analysed composition of the total plasma and IgG N-glycomes in 107 patients undergoing cardiac surgery. In nearly all individuals plasma N-glycome underwent the same pattern of changes in the first 72 h, revealing a general mechanism of glycosylation changes. To the contrary, changes in the IgG glycome were very individualized. Bi-clustering analysis revealed the existence of four distinct patterns of changes. One of them, characterized by a rapid increase in galactosylated glycoforms, was associated with nearly double mortality risk measured by EuroSCORE II. Our results indicate that individual variation in IgG glycosylation changes during acute systemic inflammation associates with increased mortality risk and indicates new avenues for the development of personalized diagnostic and therapeutic approach.
authors.affiliation: Genos Glycoscience Laboratory, Zagreb, Croatia.
authors.forename: Mislav
authors.initials: M
authors.lastname: Novokmet
authors.affiliation: Department of Orthopaedic Surgery University Hospital Centre Zagreb, Croatia.
authors.forename: Edita
authors.initials: E
authors.lastname: Lukić
authors.affiliation: Genos Glycoscience Laboratory, Zagreb, Croatia.
authors.forename: Frano
authors.initials: F
authors.lastname: Vučković
authors.affiliation: Clinic for Cardiac Surgery, University Hospital Centre Zagreb, Croatia.
authors.forename: Željko
authors.initials: Ž
authors.lastname: Ðurić
authors.affiliation: University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Biochemistry and Molecular Biology, Zagreb, Croatia.
authors.forename: Toma
authors.initials: T
authors.lastname: Keser
authors.affiliation: University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Biochemistry and Molecular Biology, Zagreb, Croatia.
authors.forename: Katarina
authors.initials: K
authors.lastname: Rajšl
authors.affiliation: Department of Physics and Astronomy DIFA, University of Bologna, Bologna, Italy.
authors.forename: Daniel
authors.initials: D
authors.lastname: Remondini
authors.affiliation: Department of Physics and Astronomy DIFA, University of Bologna, Bologna, Italy.
authors.forename: Gastone
authors.initials: G
authors.lastname: Castellani
authors.affiliation: Clinic for Cardiac Surgery, University Hospital Centre Zagreb, Croatia.
authors.forename: Hrvoje
authors.initials: H
authors.lastname: Gašparović
authors.affiliation: 1] University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Biochemistry and Molecular Biology, Zagreb, Croatia [2].
authors.forename: Olga
authors.initials: O
authors.lastname: Gornik
authors.affiliation: 1] Genos Glycoscience Laboratory, Zagreb, Croatia [2] University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Biochemistry and Molecular Biology, Zagreb, Croatia [3].
authors.forename: Gordan
authors.initials: G
authors.lastname: Lauc
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002240:Carbohydrate Sequence; D006348:Cardiac Surgical Procedures / Q000009:adverse effects* / Q000401:mortality; D002851:Chromatography, High Pressure Liquid; D016000:Cluster Analysis; D005260:Female; D054794:Glycomics; D006031:Glycosylation; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007074:Immunoglobulin G / Q000737:chemistry* / Q000276:immunology; D007249:Inflammation / Q000097:blood / Q000209:etiology / Q000276:immunology* / Q000401:mortality; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry* / Q000276:immunology; D012307:Risk Factors; D016019:Survival Analysis; D006023:Glycoproteins
chemical_list: D007074:Immunoglobulin G; D011134:Polysaccharides; C046179:glycosylated IgG; D006023:Glycoproteins