title: An evaluation of fatty acid-CoA ligase 4 in breast cancer.
abstract: BACKGROUND
  Fatty acid-CoA ligase 4 (FACL4) has been detected in various types of tumors. However, there is still very limited information about the role of FACL4 in breast cancer. Tissue microarray (TMA) technique analyzes thousands of specimens in a parallel fashion with minimal damage to the original blocks. This study was designed with the application of TMA to analyze the FACL4 status in breast cancer.
  
  
  METHODS
  Archival tissue specimens from 102 patients with primary invasive breast cancer were selected and FACL4 expression was analyzed by immunhistochemical staining with TMA. The data of primary tumor staging, age, estrogen receptor status, lymph node status, histological grading and TNM staging were also collected.
  
  
  RESULTS
  As shown my multivariate analysis, TNM stage was significantly related to the overall five-year survival rate. Nevertheless, FACL4 expression failed to have any significant relationship to overall five-year survival.
  
  
  CONCLUSIONS
  Immuno-histochemical staining with TMA was convenient and feasible for the analysis of FACL4 expression status in breast cancer. Our preliminary results showed that FACL4 expression had no significant prognostic value in breast cancer.
authors.affiliation: Professor of Surgery, Department of Surgery, Kaohsiung Chang Gung Memorial Hospital, 123, Ta-Pei Road, Niao-Sung, Kaohsiung, Taiwan. smsheen@yahoo.com.
authors.forename: Chin-Hua
authors.initials: CH
authors.lastname: Tsai
authors.forename: Shyr-Ming
authors.initials: SM
authors.lastname: Sheen-Chen
authors.forename: Chun-Ying
authors.initials: CY
authors.lastname: Huang
authors.forename: Ying-Ju
authors.initials: YJ
authors.lastname: Chen
authors.forename: Shun-Yu
authors.initials: SY
authors.lastname: Chi
authors.forename: Sheung-Fat
authors.initials: SF
authors.lastname: Ko
authors.forename: Yi-Wei
authors.initials: YW
authors.lastname: Lee
authors.forename: Jiun-Lung
authors.initials: JL
authors.lastname: Liang
authors.forename: Hsien-Wen
authors.initials: HW
authors.lastname: Hsu
authors.forename: Eng-Yen
authors.initials: EY
authors.lastname: Huang
authors.forename: Hui-Chun
authors.initials: HC
authors.lastname: Chen
authors.forename: Chao-Cheng
authors.initials: CC
authors.lastname: Huang
authors.forename: Kung-Ming
authors.initials: KM
authors.lastname: Rau
authors.forename: Rei-Ping
authors.initials: RP
authors.lastname: Tang
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001943:Breast Neoplasms / Q000201:enzymology* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000201:enzymology* / Q000473:pathology; D018275:Carcinoma, Lobular / Q000201:enzymology* / Q000473:pathology; D003066:Coenzyme A Ligases / Q000378:metabolism*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011379:Prognosis; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism; D011980:Receptors, Progesterone / Q000378:metabolism; D015996:Survival Rate; D046888:Tissue Array Analysis; D000098859:Long-Chain-Fatty-Acid-CoA Ligase
chemical_list: D014408:Biomarkers, Tumor; D003066:Coenzyme A Ligases; D018719:Erb-b2 Receptor Tyrosine Kinases; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D000098859:Long-Chain-Fatty-Acid-CoA Ligase; C508053:ERBB2 protein, human
keywords: FACL4; breast cancer; tissue microarrays