title: Activated omentum slows progression of CKD.
abstract: Stem cells show promise in the treatment of AKI but do not survive long term after injection. However, organ repair has been achieved by extending and attaching the omentum, a fatty tissue lying above the stomach containing stem cells, to various organs. To examine whether fusing the omentum to a subtotally nephrectomized kidney could slow the progression of CKD, we used two groups of rats: an experimental group undergoing 5/6 nephrectomy only and a control group undergoing 5/6 nephrectomy and complete omentectomy. Polydextran gel particles were administered intraperitoneally before suture only in the experimental group to facilitate the fusion of the omentum to the injured kidney. After 12 weeks, experimental rats exhibited omentum fused to the remnant kidney and had lower plasma creatinine and urea nitrogen levels; less glomerulosclerosis, tubulointerstitial injury, and extracellular matrix; and reduced thickening of basement membranes compared with controls. A fusion zone formed between the injured kidney and the omentum contained abundant stem cells expressing stem cell antigen-1, Wilms' tumor 1 (WT-1), and CD34, suggesting active, healing tissue. Furthermore, kidney extracts from experimental rats showed increases in expression levels of growth factors involved in renal repair, the number of proliferating cells, especially at the injured edge, the number of WT-1-positive cells in the glomeruli, and WT-1 gene expression. These results suggest that contact between the omentum and injured kidney slows the progression of CKD in the remnant organ, and this effect appears to be mediated by the presence of omental stem cells and their secretory products.
authors.affiliation: Division of Nephrology, John H. Stroger Jr. Hospital of Cook County, Chicago, Illinois; Hektoen Institute of Medicine, Chicago, Illinois; and Division of Nephrology, Department of Medicine, University of Illinois Medical Center at Chicago, Illinois; and.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Garcia-Gomez
authors.affiliation: Hektoen Institute of Medicine, Chicago, Illinois; and.
authors.forename: Nishit
authors.initials: N
authors.lastname: Pancholi
authors.affiliation: Hektoen Institute of Medicine, Chicago, Illinois; and.
authors.forename: Jilpa
authors.initials: J
authors.lastname: Patel
authors.affiliation: Division of Nephrology, John H. Stroger Jr. Hospital of Cook County, Chicago, Illinois;
authors.forename: Krishnamurthy P
authors.initials: KP
authors.lastname: Gudehithlu
authors.affiliation: Hektoen Institute of Medicine, Chicago, Illinois; and.
authors.forename: Periannan
authors.initials: P
authors.lastname: Sethupathi
authors.affiliation: Division of Nephrology, John H. Stroger Jr. Hospital of Cook County, Chicago, Illinois; Hektoen Institute of Medicine, Chicago, Illinois; and.
authors.forename: Peter
authors.initials: P
authors.lastname: Hart
authors.affiliation: Division of Nephrology, John H. Stroger Jr. Hospital of Cook County, Chicago, Illinois; Hektoen Institute of Medicine, Chicago, Illinois; and Division of Nephrology, Department of Medicine, University of Illinois Medical Center at Chicago, Illinois; and.
authors.forename: George
authors.initials: G
authors.lastname: Dunea
authors.affiliation: Division of Nephrology, John H. Stroger Jr. Hospital of Cook County, Chicago, Illinois; Hektoen Institute of Medicine, Chicago, Illinois; and Division of Nephrology, Department of Medicine, University of Illinois Medical Center at Chicago, Illinois; and Jesse Brown Chicago Veterans Affairs Medical Center, Chicago, Illinois.
authors.forename: Jose A L
authors.initials: JA
authors.lastname: Arruda
authors.affiliation: Division of Nephrology, John H. Stroger Jr. Hospital of Cook County, Chicago, Illinois; Hektoen Institute of Medicine, Chicago, Illinois; and Division of Nephrology, Department of Medicine, University of Illinois Medical Center at Chicago, Illinois; and singhashok100@yahoo.com.
authors.forename: Ashok K
authors.initials: AK
authors.lastname: Singh
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000502:physiology / Q000601:surgery; D053687:Adult Stem Cells / Q000166:cytology / Q000502:physiology*; D000818:Animals; D049109:Cell Proliferation; D004195:Disease Models, Animal; D018450:Disease Progression; D005920:Glomerular Mesangium / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D005923:Glomerulosclerosis, Focal Segmental / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D008297:Male; D009392:Nephrectomy; D009852:Omentum / Q000166:cytology / Q000502:physiology* / Q000601:surgery; D019899:Paracrine Communication / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D051436:Renal Insufficiency, Chronic / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*
chemical_list: D036341:Intercellular Signaling Peptides and Proteins