title: The effects of subclinical hypothyroidism on serum lipid level and TLR4 expression of monocyte in peripheral blood of rats.
abstract: OBJECTIVE
  To observe effect of subclinical hypothyroidism (SCH) on serum lipid level and expression of toll-like receptor 4 (TLR4) in rats' peripheral blood mononuclear cells (PBMC).
  
  
  METHODS
  Fifty Wistar female rats were divided into three groups: normal control (NC group; n=10), sham group (n=10), and L-T-4 (L-thyroxine) group (n=30, with thyroidectomy, fed with rich-calcium water after operation. 5 weeks later, abdominal subcutaneous injection of L-T-4: 0.95 μg/100g/d). 8 weeks later, the rats were killed then the peripheral blood was collected to determine the levels of serum thyroid-stimulating hormone (TSH), total thyroid hormone (TT4), total cholesterol (TC) and low density lipoprotein cholesterin (LDL-C). Rats in L-T-4 group were divided into normal lipid (NL) group) and high lipid (HL) group) according to lipid value of NC group. Monocytes were separated from blood to determine TLR4 expression by flow cytometry.
  
  
  RESULTS
  In NL and HL groups TSH were higher than in NC and Sham groups (p<0.05). TT4 have no significant differences (p>0.05). TLR4, TLR4 mRNA, NF-κB (p65) were increased (p<0.05). TNF-α, IL-6 and IL-1β were higher than in NC and sham groups (p<0.01). There were no significant differences of TLR4, TLR4 mRNA, NF-κB (p65), TNF-α, IL-6 and IL-1β expression between NL and HL groups (p>0.05).
  
  
  CONCLUSIONS
  TLR4, TLR4 mRNA, NF-κB (p65) of PBMC and TNF-α, IL-6, IL-1β expression in serum were all increased in SCH rats, which was not related to serum dyslipidemia.
authors.affiliation: Department of Cardiology, Central Hospital, Fengxian District, Shanghai 201400, China.
authors.forename: Shuan-suo
authors.initials: SS
authors.lastname: Yang
authors.affiliation: Shanghai Institute of Health Sciences, Shanghai 201318, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Tang
authors.affiliation: Department of Cardiology, Shanghai Chest Hospital, Shanghai 200030, China.
authors.forename: Ruo-gu
authors.initials: RG
authors.lastname: Li
authors.affiliation: Department of Cardiology, Central Hospital, Fengxian District, Shanghai 201400, China.
authors.forename: Guang-hao
authors.initials: GH
authors.lastname: Ge
authors.affiliation: Department of Cardiology, Shanghai Chest Hospital, Shanghai 200030, China.
authors.forename: Xin-kai
authors.initials: XK
authors.lastname: Qu
authors.affiliation: Department of Cardiology, Central Hospital, Fengxian District, Shanghai 201400, China.
authors.forename: Jiang-wei
authors.initials: JW
authors.lastname: Ma
authors.affiliation: Department of Cardiology, Central Hospital, Fengxian District, Shanghai 201400, China.
authors.forename: Zeng-yong
authors.initials: ZY
authors.lastname: Qiao
authors.affiliation: Department of Cardiology, Central Hospital, Fengxian District, Shanghai 201400, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, Central Hospital, Fengxian District, Shanghai 201400, China.
authors.forename: Hua-jin
authors.initials: HJ
authors.lastname: Liu
authors.affiliation: Department of Cardiology, Central Hospital, Fengxian District, Shanghai 201400, China.
authors.forename: Yue-mei
authors.initials: YM
authors.lastname: Hou
authors.affiliation: Department of Cardiology, Central Hospital, Fengxian District, Shanghai 201400, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Cao
authors.affiliation: Department of Cardiology, Central Hospital, Fengxian District, Shanghai 201400, China.
authors.forename: Zhi-min
authors.initials: ZM
authors.lastname: Hao
authors.affiliation: Department of Cardiology, Central Hospital, Fengxian District, Shanghai 201400, China.
authors.forename: Wen-bo
authors.initials: WB
authors.lastname: Cheng
authors.affiliation: Department of Cardiology, Central Hospital, Fengxian District, Shanghai 201400, China.
authors.forename: Hong-wei
authors.initials: HW
authors.lastname: Wang
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000096:biosynthesis / Q000097:blood; D008078:Cholesterol, LDL / Q000096:biosynthesis / Q000097:blood; D016207:Cytokines / Q000096:biosynthesis / Q000097:blood; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D007037:Hypothyroidism / Q000097:blood / Q000276:immunology* / Q000473:pathology*; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D013963:Thyroid Hormones / Q000096:biosynthesis / Q000097:blood; D013972:Thyrotropin / Q000096:biosynthesis / Q000097:blood; D013974:Thyroxine / Q000008:administration & dosage / Q000096:biosynthesis / Q000097:blood / Q000633:toxicity; D051197:Toll-Like Receptor 4 / Q000096:biosynthesis* / Q000097:blood*
chemical_list: D008078:Cholesterol, LDL; D016207:Cytokines; D012333:RNA, Messenger; D013963:Thyroid Hormones; C495347:Tlr4 protein, rat; D051197:Toll-Like Receptor 4; D013972:Thyrotropin; D002784:Cholesterol; D013974:Thyroxine