title: Mass balance approaches to characterizing the leaching potential of trenbolone acetate metabolites in agro-ecosystems.
abstract: Several studies have documented the occurrence and fate of trenbolone acetate (TBA) metabolites in soil and water. However, considerable uncertainty still exists with respect to TBA risk in agro-ecosystems because limited data are available to quantify excretion, transformation, and leaching processes. To address these uncertainties, we used experimental mesocosms and a mass balance approach to estimate the TBA metabolite leaching potential from manure excreted by implanted (40 mg TBA, 8 mg 17β-estradiol) beef cattle. Manure sample analysis indicates that over 113 days, a maximum of 9.3% (3,200 μg/animal unit [AU]) of the implant dose was excreted as 17α-trenbolone (17α-TBOH), and <1% was excreted as 17β-trenbolone (65 μg/AU) or trendione (3 μg/AU). While most (>97%) of the total excreted mass of 17α-TBOH transforms to uncharacterized products, 0.3-0.6% (100-220 μg/AU) of the implant dose accumulates on land surfaces and is available for subsequent transport. During rainfall or irrigation events, a maximum of 0.005-0.06% (1.6-22 μg/AU 17α-TBOH) or 0.005-0.012% (1.8-4 μg/AU 17α-TBOH) of the dose leached into runoff, respectively. Leaching potentials peak at 5-30 days postimplantation, suggesting that targeted timing of implantation and irrigation could minimize steroid leaching during rainfall and irrigation events.
authors.affiliation: Department of Civil and Environmental Engineering, University of Nevada-Reno , MS 0258, Reno, Nevada, 89557.
authors.forename: Gerrad D
authors.initials: GD
authors.lastname: Jones
authors.forename: Peter V
authors.initials: PV
authors.lastname: Benchetler
authors.forename: Kenneth W
authors.initials: KW
authors.lastname: Tate
authors.forename: Edward P
authors.initials: EP
authors.lastname: Kolodziej
mesh_terms: D000383:Agriculture; D000818:Animals; D001711:Biotransformation; D002417:Cattle; D017753:Ecosystem; D004958:Estradiol / Q000378:metabolism; D004963:Estrenes / Q000378:metabolism; D008372:Manure / Q000032:analysis*; D008962:Models, Theoretical; D008970:Molecular Weight; D012989:Soil Pollutants / Q000737:chemistry / Q000378:metabolism; D014204:Trenbolone Acetate / Q000097:blood / Q000737:chemistry / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004963:Estrenes; D008372:Manure; D012989:Soil Pollutants; D014874:Water Pollutants, Chemical; C070427:trendione; D004958:Estradiol; D014204:Trenbolone Acetate