title: Experimental evidence on interaction between xenon and bovine serum albumin.
abstract: Xenon gas interacts with bovine serum albumin (BSA) dissolved in a physiological buffer solution. The fluorescence quenching related to the Trp emission is reversible and depends linearly on the time of saturation by Xe. The most probable site of this interaction is Trp212. The common emission of all BSA fluorophores is also influenced by Xe but this quenching is more complex and suggests: (i) at least two sites occupied by Xe and related to the Tyr and Trp residues; (ii) structural variations of BSA induced by the Xe guest atoms.
authors.affiliation: Faculty of Chemistry, Wrocław University, Joliot Curie 14, 50-383 Wrocław, Poland.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Wołoszyn
authors.affiliation: Faculty of Chemistry, Wrocław University, Joliot Curie 14, 50-383 Wrocław, Poland. Electronic address: marek.ilczyszyn@chem.uni.wroc.pl.
authors.forename: Marek
authors.initials: M
authors.lastname: Ilczyszyn
authors.affiliation: Faculty of Chemistry, Wrocław University, Joliot Curie 14, 50-383 Wrocław, Poland.
authors.forename: Maria M
authors.initials: MM
authors.lastname: Ilczyszyn
mesh_terms: D000818:Animals; D002417:Cattle; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D012996:Solutions; D013050:Spectrometry, Fluorescence; D014978:Xenon / Q000378:metabolism*
chemical_list: D012996:Solutions; D012710:Serum Albumin, Bovine; D014978:Xenon
keywords: Albumin; Anesthesia; Fluorescence; Molecular interactions; Xenon