title: Research on sequence variations analysis of HPV-16 type in Southwestern China.
abstract: OBJECTIVE
  This study was designed to analyze sequence variations in E6, E7, and L1 of human papillomavirus type 16 (HPV-16) to identify novel HPV-6 variants and correlate them with the progression of cervical cancer.
  
  
  METHODS
  Cervical biopsies from 12 HPV-16 positive cervical neoplasia cases were analyzed by polymerase chain reaction (PCR) for E6,E7, and L1 gene. The products of PCR were ligated into pGEM-T vector and used to transform e. coli DH5a. Finally, the sequence of the insert was determined by an automated DNA sequencer.
  
  
  RESULTS
  Compared with the European-Germanyl31 (EG131), 20 mutations were detected, of which eight mutations were detected from all the four biopsies: 131(G-A)(Gly-Arg), 178 (T-G)(Asp-Glu),350 (G-T)(Val-Leu), 647 (A-G)(Asn-Asp),846 (T-C) (synonymous mutation), L1 966th (C-T)(synonymous mutation),L1 1302 (C-T)(synonymous mutation) and L1 1434th (A-G) (synonymous mutation).
  
  
  CONCLUSIONS
  Compared with other HPV-16 reference sequences, nucleotide sequencing analysis of the HPV-16, E6,E7, and L1 gene from cervical carcinoma biopses in Southwestern China consistently shows point mutations, including synonymous mutation and nonsynonymous mutation. Above all, the results may have implications for future researches of viral persistence, transmission, oncogenic potential, developing diagnostic probe, and designing vaccine or biological preparation for a particular population.
authors.affiliation: Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, Institute of Medical Genetics, College of Life Sciences, Sichuan University, Chengdu, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Laboratory, PengZhou People's Hospital, Pengzhou, China.
authors.forename: X
authors.initials: X
authors.lastname: Ding
authors.affiliation: Population and Family Planning of Chongqing Science and Technology Research Institute/Birth Defects and Reproductive Health of the Key Laboratory of Chongqing, Chongqing, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D000328:Adult; D036022:Capsid Proteins / Q000235:genetics; D002277:Carcinoma / Q000821:virology*; D002681:China; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics*; D006801:Humans; D008875:Middle Aged; D009154:Mutation; D009856:Oncogene Proteins, Viral / Q000235:genetics; D050725:Papillomavirus E7 Proteins / Q000235:genetics; D058974:Phylogeography; D012097:Repressor Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA; D002583:Uterine Cervical Neoplasms / Q000821:virology*; D055815:Young Adult
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16; C063317:L1 protein, Human papillomavirus type 16