title: FISH in micronucleus test demonstrates aneugenic action of rotenone in a common freshwater fish species, Nile tilapia (Oreochromis niloticus).
abstract: Aneuploidies are numerical genetic alterations that lead to changes in the normal number of chromosomes due to abnormal segregation during cell division. This type of alteration can occur spontaneously or as a result of exposure to mutagenic agents. The presence of these agents in the environment has increased concern about potential damage to human health. Rotenone, derived from plants of the genera Derris and Lonchocarpus, is a product that is used all over the world as a pesticide and piscicide. Before establishing its potential and efficiency for these purposes, it is essential to know more about the possible adverse effects that it may cause. The current work aimed to evaluate the mutagenic potential of rotenone using fish from the species Oreochromis niloticus, as well as to help in understanding its action mechanism. Our results showed the mutagenic potential of rotenone evidenced by increased formation of micronuclei and nuclear buds at low doses of exposure. The use of fluorescence in situ hybridisation technique made it possible to measure the aneugenic potential of the substance, probably due to its impairment of mitotic spindle formation.
authors.affiliation: Laboratório de Citogenética, Departamento de Genética, Instituto de Ciências Biológicas, Universidade Federal do Pará, Campus do Guamá, Av. Perimetral, sn., Guamá, 66075-900 Belém, PA-Brazil.
authors.forename: Karina M
authors.initials: KM
authors.lastname: Melo
authors.forename: Cesar K
authors.initials: CK
authors.lastname: Grisolia
authors.forename: Julio C
authors.initials: JC
authors.lastname: Pieczarka
authors.forename: Ludmilla R
authors.initials: LR
authors.lastname: de Souza
authors.forename: José de Souza
authors.initials: Jde S
authors.lastname: Filho
authors.forename: Cleusa Y
authors.initials: CY
authors.lastname: Nagamachi
mesh_terms: D024422:Aneugens / Q000633:toxicity*; D000782:Aneuploidy; D000818:Animals; D001938:Brazil; D023681:Cichlids / Q000235:genetics*; D053938:DNA Fragmentation / Q000187:drug effects; D005260:Female; D005398:Fisheries; D005618:Fresh Water; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D015162:Micronucleus Tests / Q000379:methods*; D010575:Pesticides / Q000633:toxicity; D012402:Rotenone / Q000633:toxicity*; D008941:Spindle Apparatus / Q000187:drug effects
chemical_list: D024422:Aneugens; D010575:Pesticides; D012402:Rotenone