title: Antitumor metastasis pregnane alkaloids from Pachysandra terminalis.
abstract: Three new pregnane alkaloids, named terminamines H-J (1-3), together with two known alkaloids (4 and 5), were isolated from the ethanol extract of Pachysandra terminalis. The structures of isolated compounds were elucidated by spectroscopic methods, including (1)H and (13)C NMR, 2D NMR, and HR-ESI-MS. Compounds 1, 4, and 5 revealed significant anti-metastasis activities. In addition, compound 1 inhibited the expression of p-PKCζ in MDA-MB-231 cells, and compound 4 inhibited the expressions of p-PKCζ in MDA-MB-231 and A549 cells.
authors.affiliation: a Research Center of Basic Medical Sciences, Tianjin Medical University , Tianjin 300070 , China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Zhao
authors.forename: Chun-Chun
authors.initials: CC
authors.lastname: Gan
authors.forename: Mei-Na
authors.initials: MN
authors.lastname: Jin
authors.forename: Sheng-An
authors.initials: SA
authors.lastname: Tang
authors.forename: Nan
authors.initials: N
authors.lastname: Qin
authors.forename: Hong-Quan
authors.initials: HQ
authors.lastname: Duan
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D031236:Pachysandra / Q000737:chemistry*; D011278:Pregnanes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D011278:Pregnanes; C000589347:terminamine H; C000589348:terminamine I; C000589349:terminamine J
keywords: PKCζ; Pachysandra terminalis; antitumor metastasis; pregnane alkaloids