title: Method Validation for the One-Month Stability Study of trans-Resveratrol in Human Plasma.
abstract: BACKGROUND
  trans-Resveratrol (t-Res) is a natural phenolic compound with various biological activities such as antioxidant, anticancer, cancer chemopreventive, cardioprotective, and neuroprotective.
  
  
  OBJECTIVE
  There is no comprehensive study about the stability of t-Res in human plasma. With respect to extensive use of t-Res in clinical trials and pharmacokinetic studies, in this study, we aimed to measure the plasma stability of t-Res at different conditions of lighting, pH, and temperature, and in the presence of routine antioxidant agents (BHT and VIT C).
  
  
  METHODS
  t-Res stability in human plasma was evaluated at different conditions of lighting, pH and temperature, and in the presence of routine antioxidant agents (BHT and VIT C), and quantitatively determined using HPLC-UV method at 306 nm.
  
  
  RESULTS
  Our findings revealed that t-Res was quite stable in different temperatures ranging from -70 °C to 25 °C, and acidic pH conditions in plasma for a month, when protected from light, but it was unstable in alkali pH and in lighting conditions.
  
  
  CONCLUSIONS
  In lighting conditions, most of t-Res was isomerized to the other isomer cis-resveratrol. t-Res is unstable in alkali pH and when exposed to light. t-Res was quite stable at other conditions, and several freeze-thaw cycles did not have any effect on its stability.
authors.affiliation: Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, IR Iran ; Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors.forename: Milad
authors.initials: M
authors.lastname: Iranshahy
authors.affiliation: Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors.forename: Amir Hooshang
authors.initials: AH
authors.lastname: Mohammadpoor
authors.affiliation: Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Hassanzadeh-Khayyat
authors.affiliation: Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, IR Iran ; Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Iranshahi
keywords: Antioxidants; Resveratrol; Stability