title: Cytotoxic effect of silorane and methacrylate based composites on the human dental pulp stem cells and fibroblasts.
abstract: OBJECTIVE
  The aim of this study was to compare the cytotoxic effect of a methacrylate-based and a silorane-based composite on the human dental pulp stem cells (DPSCs) versus human dental pulp fibroblasts (DPFs).
  
  
  METHODS
  Samples of the Filtek Z250 and P90 were polymerized and immersed in the culture medium to obtain extracts after incubation for one, seven and 14 days. Magnetic cell sorting based on the CD146 expression was performed to purify DPSCs and DPFs. After incubation of both cells with the extracts, cytotoxicity was determined using the MTT test.
  
  
  RESULTS
  For the extracts of first and seventh day, both composites showed significantly lower cytotoxicity on DPSCs than DPFs (p=0.003). In addition, there was a significant difference in the time-group interaction of both materials indicating different cytotoxic behaviours (p=0.014). In contrast to Z250, exposure to the 14th day extract of P90 resulted in higher cell viability compared to that of day seven.
  
  
  CONCLUSIONS
  DPSCs are less susceptible to the cytotoxic effect of the composites than DPFs. Compared to Z250, the cytotoxic effect of silorane-based composite decreases as the time passes on. This difference should be considered, particularly in deep cavities, in order to preserve the regenerative capacity of the pulp.
authors.affiliation: Department of Operative Dentistry, Dental Faculty, Ghasrdasht Street, 71345-1836, Shiraz, Iran, tavangarm@sums.ac.ir.
authors.forename: Fereshteh
authors.initials: F
authors.lastname: Shafiei
authors.forename: Maryam S
authors.initials: MS
authors.lastname: Tavangar
authors.forename: Mahboobeh
authors.initials: M
authors.lastname: Razmkhah
authors.forename: Armin
authors.initials: A
authors.lastname: Attar
authors.forename: Ali-Asghar
authors.initials: AA
authors.lastname: Alavi
mesh_terms: D003188:Composite Resins / Q000009:adverse effects*; D003782:Dental Pulp / Q000166:cytology*; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D008689:Methacrylates / Q000009:adverse effects*; D062047:Silorane Resins / Q000009:adverse effects*; D013234:Stem Cells / Q000187:drug effects*
chemical_list: D003188:Composite Resins; C444701:Filtek Z250; D008689:Methacrylates; D062047:Silorane Resins; C544925:filtek P90