title: A novel targeting drug carrier to deliver chemical bonded and physical entrapped anti-tumor drugs.
abstract: In this study, we demonstrated a novel targeting drug carrier formed by amphiphilic prodrug based mixed micelle. Along with octadecyl chains, chemical bonded Dox moieties were utilized to entrap free drugs (PTX) and simultaneously acted as therapeutic agents. The formulation of CP-Folate/CPN=Dox/PTX showed spherical micellar structure and possessed high drug loading content, which was up to 22.9%. We examined the cell uptaken capacity and the cytotoxicity of mixed micelle by CLSM and MTT assay. The introducing of folate moiety enhanced intracellular accumulation in HeLa cells and co-delivery of Dox and PTX showed stronger anti-tumor activity even compared with free drugs.
authors.affiliation: Wuhan Docan Pharmaceutical Co., Ltd., Wuhan 430040, China; Pharmacy School of Tongji Medical college, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Pharmacy, Renmin Hospital of Wuhan University, Wuhan 430060, China. Electronic address: songjcwhu@163.com.
authors.forename: Jinchun
authors.initials: J
authors.lastname: Song
authors.affiliation: Pharmacy School of Tongji Medical college, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Bangyin
authors.initials: B
authors.lastname: Chen
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry; D004337:Drug Carriers / Q000008:administration & dosage* / Q000737:chemistry; D005492:Folic Acid / Q000008:administration & dosage* / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008823:Micelles; D017239:Paclitaxel / Q000008:administration & dosage* / Q000737:chemistry; D011092:Polyethylene Glycols / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D008823:Micelles; D011092:Polyethylene Glycols; D004317:Doxorubicin; D005492:Folic Acid; D017239:Paclitaxel
keywords: Anti-tumor drugs; Drug carrier; Entrapped; Targeting