title: Galectin-3 contributes to cisplatin-induced myeloid derived suppressor cells (MDSCs) recruitment in Lewis lung cancer-bearing mice.
abstract: Recently the recruitment/migration of myeloid derived suppressor cells (MDSCs) to tumor microenvironment after chemotherapy has attracted much attention. To determine the detailed mechanism for the responses of MDSCs to these chemotherapies, we investigated the changes of galectin-3 and MDSCs in response to cisplatin(0.4 mg/kg, 4 mg/kg) treatment both in vivo and ex vivo. In the process of cisplatin, we assessed levels of galectin-3 and MDSCs in the Lewis lung cancer (LLC) bearing mice using immunohistochemistry, enzyme-linked immunosorbent assay (ELISA), immunofluorence and flow cytometry (FCM). The expression and changes of galectin-3 in the LLC cell line were detected by western blot, immunofluorence and ELISA. The ligand for galectin-3 on MDSCs and the chemotaxis of galectin-3 to MDSCs were confirmed using FCM and transwell. Parallel increased level of galectin-3 with the number of MDSCs in vivo was detected after cisplatin treatment. LLC cells expressed galectin-3 and cisplatin increased galectin-3 level in the culture medium. Furthermore, MDSCs were detected to express CD98, the ligand of galectin-3, and could be recruited by galectin-3. Our results suggested that the elevated expression of gelectin-3 in LLC tumor cells may contribute to the migration of MDSCs to the tumor microenvironment in response to cisplatin.
authors.affiliation: Department of Oncology, Huashan Hospital, Fudan University, No. 12, Wulumuqi Zhong Road, Shanghai, 200040, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Wang
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Chu
authors.forename: Hao
authors.initials: H
authors.lastname: Lin
authors.forename: Jingwei
authors.initials: J
authors.lastname: Jiang
authors.forename: Xinli
authors.initials: X
authors.lastname: Zhou
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Liang
mesh_terms: D000818:Animals; D018827:Carcinoma, Lewis Lung / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000009:adverse effects*; D005434:Flow Cytometry; D037502:Galectin 3 / Q000096:biosynthesis* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008024:Ligands; D051379:Mice; D022423:Myeloid Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D059016:Tumor Microenvironment / Q000187:drug effects*
chemical_list: D037502:Galectin 3; C485095:Lgals3 protein, mouse; D008024:Ligands; D002945:Cisplatin