title: Differential expression of Toll-like receptor pathway genes in chicken embryo fibroblasts from chickens resistant and susceptible to Marek's disease.
abstract: The Toll-like receptor (TLR) signaling pathway is one of the innate immune defense mechanisms against pathogens in vertebrates and invertebrates. However, the role of TLR in non-MHC genetic resistance or susceptibility to Marek's disease (MD) in the chicken is yet to be elucidated. Chicken embryo fibroblast (CEF) cells from MD susceptible and resistant lines were infected either with Marek's disease virus (MDV) or treated with polyionosinic-polycytidylic acid, a synthetic analog of dsRNA, and the expression of TLR and pro-inflammatory cytokines was studied at 8 and 36 h posttreatment by quantitative reverse transcriptase PCR. Findings of the present study reveal that MDV infection and polyionosinic-polycytidylic acid treatment significantly elevated the mRNA expression of TLR3, IL6, and IL8 in both susceptible and resistant lines. Furthermore, basal expression levels in uninfected CEF for TLR3, TLR7, and IL8 genes were significantly higher in resistant chickens compared with those of susceptible chickens. Our results suggest that TLR3 together with pro-inflammatory cytokines may play a significant role in genetic resistance to MD.
authors.affiliation: Directorate of Poultry Research, Rajendranagar, Hyderabad-500 030, India.
authors.forename: Santosh
authors.initials: S
authors.lastname: Haunshi
authors.forename: Hans H
authors.initials: HH
authors.lastname: Cheng
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics* / Q000378:metabolism; D002642:Chick Embryo; D002645:Chickens; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D004198:Disease Susceptibility / Q000276:immunology / Q000378:metabolism / Q000662:veterinary; D005347:Fibroblasts; D018507:Gene Expression Regulation, Developmental; D025961:Herpesvirus 3, Gallid / Q000502:physiology; D008380:Marek Disease / Q000235:genetics* / Q000276:immunology / Q000821:virology; D011070:Poly I-C / Q000008:administration & dosage; D011201:Poultry Diseases / Q000235:genetics* / Q000276:immunology / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D015398:Signal Transduction; D051193:Toll-Like Receptors / Q000235:genetics* / Q000378:metabolism
chemical_list: D030161:Avian Proteins; D016207:Cytokines; D051193:Toll-Like Receptors; D011070:Poly I-C
keywords: Marek’s disease; Toll-like receptor; cytokine; resistance