title: Gamma histone 2AX (γ-H2AX)as a predictive tool in radiation oncology.
abstract: Ionizing radiation cause DNA damage to cells, leading them to cell death via DNA double-strand breaks (DSBs) formation. DSBs formation is followed immediately by histone H2AX phosphorylation (γ-H2AX) and multitude repair factors accumulation. Here we present the methods and the bio-sampling for γ-H2AX detection, γ-H2AX formation in normal cells and animal tissues, in cancer cell lines/tissues and in clinical trials after radiation treatment, alone or in combination with other factors. The purpose of this review is to highlight the use of γ-H2AX, as a marker to assess DNA damage and repair.
authors.affiliation: Department of Radiotherapy/Oncology, Radiobiology and Radiopathology Unit, Democritus University of Thrace , Alexandroupolis , Greece.
authors.forename: Stamatia
authors.initials: S
authors.lastname: Pouliliou
authors.forename: Michael I
authors.initials: MI
authors.lastname: Koukourakis
mesh_terms: D000818:Animals; D004249:DNA Damage; D004260:DNA Repair; D006657:Histones / Q000378:metabolism*; D006801:Humans; D018787:Radiation Oncology
chemical_list: C468783:H2AX protein, human; D006657:Histones