title: A new method to fabricate an electrochemical aptasensor to assay adenosine deaminase concentration using an assistance DNA.
abstract: A novel strategy for the fabrication of electrochemical aptasensor is proposed in this work. This strategy has been employed to develop an aptasensor for the detection of adenosine deaminase concentration. A probe which contains adenosine aptamer and modified with ferrocene is adopted in our strategy as the core element. Moreover, an assistance DNA which can hybridize with the probe was also been employed in our strategy. The enzymatic reaction of adenosine catalyzed by adenosine deaminase plays a key role as well in the regulation of the hybridized complex. The formation of these regions of rigid, duplex DNA prevents the ferrocene tag from approaching the electrode surface, suppressing amperometric currents. The electroactive probe is to reflect the concentration of the enzyme indirectly but accurately. The detection limit is 1 U L(-1), which can be acceptable for clinical applications.
authors.affiliation: a Department of Emergency , Nanjing Medical University, Affiliated Wuxi People's Hospital , Wuxi , Jiangsu , China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Huang
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Zheng
mesh_terms: D000243:Adenosine Deaminase / Q000097:blood* / Q000378:metabolism; D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004789:Enzyme Activation; D006801:Humans
chemical_list: D052157:Aptamers, Nucleotide; D004247:DNA; D000243:Adenosine Deaminase
keywords: adenosine deaminase; aptamer; electrochemistry; ferrocene