title: Multiple mechanistically distinct modes of endocannabinoid mobilization at central amygdala glutamatergic synapses.
abstract: The central amygdala (CeA) is a key structure at the limbic-motor interface regulating stress responses and emotional learning. Endocannabinoid (eCB) signaling is heavily implicated in the regulation of stress-response physiology and emotional learning processes; however, the role of eCBs in the modulation of synaptic efficacy in the CeA is not well understood. Here we describe the subcellular localization of CB1 cannabinoid receptors and eCB synthetic machinery at glutamatergic synapses in the CeA and find that CeA neurons exhibit multiple mechanistically and temporally distinct modes of postsynaptic eCB mobilization. These data identify a prominent role for eCBs in the modulation of excitatory drive to CeA neurons and provide insight into the mechanisms by which eCB signaling and exogenous cannabinoids could regulate stress responses and emotional learning.
authors.affiliation: Department of Psychiatry, Vanderbilt University School of Medicine, Nashville, TN 37212, USA; Vanderbilt Brain Institute, Vanderbilt University School of Medicine, Nashville, TN 37212, USA.
authors.forename: Teniel S
authors.initials: TS
authors.lastname: Ramikie
authors.affiliation: Momentum Laboratory of Molecular Neurobiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Rita
authors.initials: R
authors.lastname: Nyilas
authors.affiliation: Department of Psychiatry, Vanderbilt University School of Medicine, Nashville, TN 37212, USA; Vanderbilt Brain Institute, Vanderbilt University School of Medicine, Nashville, TN 37212, USA.
authors.forename: Rebecca J
authors.initials: RJ
authors.lastname: Bluett
authors.affiliation: Department of Psychiatry, Vanderbilt University School of Medicine, Nashville, TN 37212, USA; Vanderbilt Brain Institute, Vanderbilt University School of Medicine, Nashville, TN 37212, USA.
authors.forename: Joyonna C
authors.initials: JC
authors.lastname: Gamble-George
authors.affiliation: Department of Psychiatry, Vanderbilt University School of Medicine, Nashville, TN 37212, USA.
authors.forename: Nolan D
authors.initials: ND
authors.lastname: Hartley
authors.affiliation: Gill Institute and Department of Psychological and Brain Sciences, Indiana University, Bloomington, IN 47405, USA.
authors.forename: Ken
authors.initials: K
authors.lastname: Mackie
authors.affiliation: Department of Anatomy, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Watanabe
authors.affiliation: Momentum Laboratory of Molecular Neurobiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: István
authors.initials: I
authors.lastname: Katona
authors.affiliation: Department of Psychiatry, Vanderbilt University School of Medicine, Nashville, TN 37212, USA; Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37212, USA; Vanderbilt Brain Institute, Vanderbilt University School of Medicine, Nashville, TN 37212, USA. Electronic address: sachin.patel@vanderbilt.edu.
authors.forename: Sachin
authors.initials: S
authors.lastname: Patel
mesh_terms: D000679:Amygdala / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D063388:Endocannabinoids / Q000378:metabolism*; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D054769:G-Protein-Coupled Receptor Kinase 2; D043802:GTP-Binding Protein alpha Subunits, Gq-G11 / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009474:Neurons / Q000378:metabolism*; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism; D043884:Receptor, Cannabinoid, CB1 / Q000378:metabolism*; D011976:Receptors, Muscarinic / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D013569:Synapses / Q000378:metabolism*; D009435:Synaptic Transmission / Q000502:physiology
chemical_list: C543264:Cnr1 protein, rat; D063388:Endocannabinoids; D043884:Receptor, Cannabinoid, CB1; D011976:Receptors, Muscarinic; D018698:Glutamic Acid; D020794:Receptor Protein-Tyrosine Kinases; C080577:muscarinic receptor kinase; D054769:G-Protein-Coupled Receptor Kinase 2; D043802:GTP-Binding Protein alpha Subunits, Gq-G11; D002118:Calcium