title: Preparation and characterization of PEG-modified PCL nanoparticles for oxygen carrier: a new application of Fourier transform infrared spectroscopy for quantitative analysis of the hemoglobin in nanoparticles.
abstract: The influence of polyethylene glycol (PEG) molar ratio on the nanoparticles (NPs) properties is described herein. Especially, a facile and nondestructive determination route has been raised to quantify the hemoglobin (Hb) amounts in NPs via an internal standard FTIR method. The subsequent results indicated that, briefly, the PEG molar ratio did negligible influence on the size distribution of NPs, however, it did have great effect on the NPs zeta potential and hydrophilicity as well as the Hb loading amount. These findings highlight that the PEG density on the surface is a key parameter affecting the NPs properties.
authors.affiliation: a School of Materials Science & Engineering, Shanghai Institute of Technology , Shanghai , PR China.
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Shan
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.forename: Changsheng
authors.initials: C
authors.lastname: Liu
mesh_terms: D000818:Animals; D001802:Blood Substitutes / Q000737:chemistry*; D002417:Cattle; D006454:Hemoglobins / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010100:Oxygen / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001802:Blood Substitutes; D006454:Hemoglobins; D011092:Polyethylene Glycols; D010100:Oxygen; C000595209:polyethylene glycol 1000
keywords: FTIR spectroscopy; hemoglobin content; polymeric nanoparticles; quantification