title: microRNA-125 distinguishes developmentally generated and adult-born olfactory bulb interneurons.
abstract: New neurons, originating from the subventricular zone, are continuously integrating into neuronal circuitry in the olfactory bulb (OB). Using a transgenic sensor mouse, we found that adult-born OB interneurons express microRNA-125 (miR-125), whereas the pre-existing developmentally generated OB interneurons represent a unique population of cells in the adult brain, without miR-125 activity. Stable inhibition of miR-125 in newborn OB neurons resulted in enhanced dendritic morphogenesis, as well as in increased synaptic activation in response to odour sensory stimuli. These data demonstrate that miR-125 controls functional synaptic integration of adult-born OB interneurons. Our results also suggest that absence of an otherwise broadly expressed miRNA is a novel mechanism with which to achieve neuronal subtype specification.
authors.affiliation: Laboratory of Molecular Neurogenetics, Department of Experimental Medical Science, Wallenberg Neuroscience Center and Lund Stem Cell Center, Lund University, 221 84 Lund, Sweden.
authors.forename: Malin
authors.initials: M
authors.lastname: Akerblom
authors.forename: Rebecca
authors.initials: R
authors.lastname: Petri
authors.forename: Rohit
authors.initials: R
authors.lastname: Sachdeva
authors.forename: Thies
authors.initials: T
authors.lastname: Klussendorf
authors.forename: Bengt
authors.initials: B
authors.lastname: Mattsson
authors.forename: Bernhard
authors.initials: B
authors.lastname: Gentner
authors.forename: Johan
authors.initials: J
authors.lastname: Jakobsson
mesh_terms: D053687:Adult Stem Cells / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D053595:Embryonic Stem Cells / Q000502:physiology*; D005260:Female; D007395:Interneurons / Q000166:cytology / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000502:physiology*; D058953:Neural Stem Cells / Q000502:physiology; D055495:Neurogenesis / Q000235:genetics; D009830:Olfactory Bulb / Q000166:cytology* / Q000196:embryology / Q000378:metabolism; D013569:Synapses / Q000235:genetics
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; C527468:Mirn125 microRNA, mouse
keywords: Mouse; Neurogenesis; Olfactory bulb; microRNA