title: Alterations in the extracellular catabolism of nucleotides and platelet aggregation induced by high-fat diet in rats: effects of α-tocopherol.
abstract: The aim of this study was to assess whether α-tocopherol administration prevented alterations in the ectonucleotidase activities and platelet aggregation induced by high-fat diet in rats. Thus, we examined four groups of male rats which received standard diet, high-fat diet (HFD), α-tocopherol (α-Toc), and high-fat diet plus α-tocopherol. HFD was administered ad libitum and α-Toc by gavage using a dose of 50 mg/kg. After 3 months of treatment, animals were submitted to euthanasia, and blood samples were collected for biochemical assays. Results demonstrate that NTPDase, ectonucleotide pyrophosphatase/phosphodiesterase, and 5'-nucleotidase activities were significantly decreased in platelets of HFD group, while that adenosine deaminase (ADA) activity was significantly increased in this group in comparison to the other groups (P < 0.05). When rats that received HFD were treated with α-Toc, the activities of these enzymes were similar to the control, but ADA activity was significantly increased in relation to the control and α-Toc group (P < 0.05). HFD group showed an increased in platelet aggregation in comparison to the other groups, and treatment with α-Toc significantly reduced platelet aggregation in this group. These findings demonstrated that HFD alters platelet aggregation and purinergic signaling in the platelets and that treatment with α-Toc was capable of modulating the adenine nucleotide hydrolysis in this experimental condition.
authors.affiliation: Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Av. Roraima, 97105-900, Santa Maria, RS, Brazil.
authors.forename: Jessié M
authors.initials: JM
authors.lastname: Gutierres
authors.forename: Fabiano B
authors.initials: FB
authors.lastname: Carvalho
authors.forename: Maria Rosa C
authors.initials: MR
authors.lastname: Schetinger
authors.forename: Marília V
authors.initials: MV
authors.lastname: Rodrigues
authors.forename: Juliano M
authors.initials: JM
authors.lastname: Vieira
authors.forename: Paula
authors.initials: P
authors.lastname: Maldonado
authors.forename: Maria do Carmo S
authors.initials: Mdo C
authors.lastname: Araújo
authors.forename: Roberta
authors.initials: R
authors.lastname: Schmatz
authors.forename: Naiara
authors.initials: N
authors.lastname: Stefanello
authors.forename: Jeandre A S
authors.initials: JA
authors.lastname: Jaques
authors.forename: Marcio
authors.initials: M
authors.lastname: Costa
authors.forename: Vera
authors.initials: V
authors.lastname: Morsch
authors.forename: Cinthia M
authors.initials: CM
authors.lastname: Mazzanti
authors.forename: Victor
authors.initials: V
authors.lastname: Pimentel
authors.forename: Sonia Terezinha A
authors.initials: ST
authors.lastname: Lopes
authors.forename: Roselia M
authors.initials: RM
authors.lastname: Spanevello
mesh_terms: D000818:Animals; D059305:Diet, High-Fat; D009711:Nucleotides / Q000378:metabolism*; D010974:Platelet Aggregation; D051381:Rats; D024502:alpha-Tocopherol / Q000494:pharmacology*
chemical_list: D009711:Nucleotides; D024502:alpha-Tocopherol