title: Quantitative determination of amisulpride in rat plasma by HPLC-MS/MS.
abstract: Amisulpride, a selective antagonist of D2 and D3 dopamine receptors, is used as an antipsychotic drug. In this study, we reported a sensitive LC-MS/MS method for determining amisulpride concentrations in rat plasma, and a preclinical pharmacokinetic study in the rat. After a simple protein precipitation with acetonitrile containing methaqualone as an internal standard, the analytes were separated on a reversed-phase column with a mobile phase of 0.2 % aqueous formic acid and acetonitrile (3:7, v/v). The accuracy and precision of the assay were in accordance with FDA guidance for the validation of bioanalytical methods. This analytical method was used successfully to characterize the time course of the plasma concentration of amisulpride following oral administration of a single 10 mg/kg dose in rats.
authors.affiliation: College of Pharmacy, Yeungnam University, Kyoungsan, Kyoungbuk, 712-749, South Korea.
authors.forename: Keumhan
authors.initials: K
authors.lastname: Noh
authors.forename: Yoo-Jeong
authors.initials: YJ
authors.lastname: Jang
authors.forename: Kwang-il
authors.initials: KI
authors.lastname: Kwon
authors.forename: Eunyoung
authors.initials: E
authors.lastname: Kim
authors.forename: Tae Cheon
authors.initials: TC
authors.lastname: Jeong
authors.forename: Hwi-yeol
authors.initials: HY
authors.lastname: Yun
authors.forename: Wonku
authors.initials: W
authors.lastname: Kang
mesh_terms: D000284:Administration, Oral; D000077582:Amisulpride; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D051381:Rats; D013469:Sulpiride / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D053719:Tandem Mass Spectrometry
chemical_list: D013469:Sulpiride; D000077582:Amisulpride