title: Age effects on plasma cholesterol and triglyceride profiles and metabolite concentrations in dogs.
abstract: BACKGROUND
  In dogs, occurrence of lipid metabolism disorders such as obesity and diabetes mellitus has increased markedly in recent years. Hyperlipidemia has been regarded as a common characteristic for obese animals and hyperlipidemic condition may be associated with inflammation, oxidative stress and lipid composition changes. In this study, we investigated the changes in plasma cholesterol and triglyceride (TG) profiles and metabolite concentrations in 24 dogs (young group: 0-7 years old, n = 12, aged group: 8-13 years old, n = 12).
  
  
  RESULTS
  Plasma adiponectin (ADN) concentrations were significantly lower in aged dogs than those in young dogs (mean ± SD, 17.2 ± 10.0 μg mL-1 vs 29.3 ± 12.5 μg mL-1, respectively; P <0.05). Although there were no significant differences statistically, aged dogs showed significantly higher plasma alpha1- acid glycoprotein (alpah1-AG) levels compared to those in young dogs. Plasma cholesterol lipoprotein and TG lipoprotein were divided into four fractions by biphasic agarose gel electrophoresis technique. The levels of the third TG-lipoprotein fraction from the positive pole (TG Fraction 3) were significantly higher in aged dogs than in young dogs (mean ± SD, 143.0 ± 109.3 mg dL-1 vs 55.2 ± 31.3 mg dL-1, respectively; P <0.05). On the correlation coefficient analysis by Peason's method, moderate positive correlations were seen between the age and TG (r = 0.446, P = 0.029), TG Fraction 3 (r = 0.516, P = 0.010), malondialdehyde (r = 0.146, P = 0.043), alpha-1 AG (r = 0.448, P = 0.028) levels, respectively. Moderate negative correlations were seen the age and total cholesterol (TC) Fraction 2 (r = -0.446, P = 0.029), glucose (r = -0.637, P = 0.001), ADN (r = -0.408, P = 0.048), respectively.
  
  
  CONCLUSIONS
  Present data suggest biochemical characteristics of lipid metabolism disorder may be affected by aging in dogs.
authors.affiliation: Department of Veterinary Science, School of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho Musashino, Tokyo 180-8602, Japan. kawasumi224@nvlu.ac.jp.
authors.forename: Koh
authors.initials: K
authors.lastname: Kawasumi
authors.forename: Nanae
authors.initials: N
authors.lastname: Kashiwado
authors.forename: Yuki
authors.initials: Y
authors.lastname: Okada
authors.forename: Masaki
authors.initials: M
authors.lastname: Sawamura
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Sasaki
authors.forename: Eiji
authors.initials: E
authors.lastname: Iwazaki
authors.forename: Nobuko
authors.initials: N
authors.lastname: Mori
authors.forename: Ichiro
authors.initials: I
authors.lastname: Yamamoto
authors.forename: Toshiro
authors.initials: T
authors.lastname: Arai
mesh_terms: D052242:Adiponectin / Q000097:blood; D000375:Aging / Q000097:blood* / Q000502:physiology; D000818:Animals; D002784:Cholesterol / Q000097:blood*; D004285:Dogs; D005260:Female; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D014280:Triglycerides / Q000097:blood* / Q000378:metabolism
chemical_list: D052242:Adiponectin; D008074:Lipoproteins; D014280:Triglycerides; D002784:Cholesterol