title: Cyr61 expression is associated with prognosis in patients with colorectal cancer.
abstract: BACKGROUND
  Cysteine-rich 61 (Cyr61), a member of the CCN protein family, possesses diverse functionality in cellular processes such as adhesion, migration, proliferation, and survival. Cyr61 can also function as an oncogene or a tumour suppressor, depending on the origin of the cancer. Only a few studies have reported Cyr61 expression in colorectal cancer. In this study, we assessed the Cyr61 expression in 251 colorectal cancers with clinical follow up.
  
  
  METHODS
  We examined Cyr61 expression in 6 colorectal cancer cell lines (HT29, Colo205, Lovo, HCT116, SW480, SW620) and 20 sets of paired normal and colorectal cancer tissues by western blot. To validate the association of Cyr61 expression with clinicopathological parameters, we assessed Cyr61 expression using tissue microarray analysis of primary colorectal cancer by immunohistochemical analysis.
  
  
  RESULTS
  We verified that all of the cancer cell lines expressed Cyr61; 2 cell lines (HT29 and Colo205) demonstrated Cyr61 expression to a slight extent, while 4 cell lines (Lovo, HCT116, SW480, SW620) demonstrated greater Cyr61 expression than HT29 and Colo205 cell lines. Among the 20 cases of paired normal and tumour tissues, greater Cyr61 expression was observed in 16 (80%) tumour tissues than in normal tissues. Furthermore, 157 out of 251 cases (62.5%) of colorectal cancer examined in this study displayed strong Cyr61 expression. Cyr61 expression was found to be associated with pN (p = 0.018). Moreover, Cyr61 expression was associated with statistically significant cancer-specific mortality (p = 0.029). The duration of survival was significantly lesser in patients with Cyr61 high expression than in patients with Cyr61 low expression (p = 0.001). These results suggest that Cyr61 expression plays several important roles in carcinogenesis and may also be a good prognostic marker for colorectal cancer.
  
  
  CONCLUSIONS
  Our data confirmed that Cyr61 was expressed in colorectal cancers and the expression was correlated with worse prognosis of colorectal cancers.
authors.forename: Dongjun
authors.initials: D
authors.lastname: Jeong
authors.forename: Suhak
authors.initials: S
authors.lastname: Heo
authors.forename: Tae
authors.initials: T
authors.lastname: Sung Ahn
authors.forename: Sookyoung
authors.initials: S
authors.lastname: Lee
authors.forename: Soyoung
authors.initials: S
authors.lastname: Park
authors.forename: Hyungjoo
authors.initials: H
authors.lastname: Kim
authors.forename: Doosan
authors.initials: D
authors.lastname: Park
authors.forename: Sang
authors.initials: S
authors.lastname: Byung Bae
authors.forename: Sung Soo
authors.initials: SS
authors.lastname: Lee
authors.forename: Moon
authors.initials: M
authors.lastname: Soo Lee
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Kim
authors.affiliation: Department of Surgery, College of Medicine, Soonchunhyang University, 31 soonchunhyang 6 gil, Dongnam-gu, Cheonan, Chungcheongnam-do 330-722, Republic of Korea. ssurge@schmc.ac.kr.
authors.forename: Moo
authors.initials: M
authors.lastname: Jun Baek
mesh_terms: D000368:Aged; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality* / Q000473:pathology; D055515:Cysteine-Rich Protein 61 / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011379:Prognosis
chemical_list: D055515:Cysteine-Rich Protein 61