title: Poly(hydroxybutyrate-co-hydroxyvalerate) bilayer skin tissue engineering constructs with improved epidermal rearrangement.
abstract: Bilayer skin substitutes constitute an attractive strategy towards improved skin wound healing. Therefore, solvent casting and freeze-drying methodologies are used to produce polyhydroxybutyrate-co-hydroxyvalerate (PHBV) thin nanoporous membranes and 3D porous scaffolds that are combined in bilayer structures to recreate the epidermal and dermal layers, respectively. The combination of these methodologies allow attaining a bilayer structure with a high water retention capability and adequate mechanical properties, susceptible to enzymes degradative action. Cultures established with human keratinocytes (hKC) and dermal fibroblasts (hDFb) confirm the suitability of the PHBV structures to support cell adhesion and proliferation. Nonetheless, when co-cultured under defined conditions, hKC are able to grow and rearrange in a multilayer structure with proliferative cells in the basal layer, and cells expressing a terminal differentiation marker in the upper layer. Therefore, PHBV bilayer structures demonstrate properties that favor skin cells performance, thus representing a promising strategy to improve wound healing.
authors.affiliation: 3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909, Taipas, Guimarães, Portugal; ICVS/3B's - PT Government Associate Laboratory Braga/Guimarães, Portugal; Laboratory of Cellular and Molecular Immunology, Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, Caixa Postal 486, CEP 31.270-901, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Zonari
authors.forename: Mariana T
authors.initials: MT
authors.lastname: Cerqueira
authors.forename: Silviene
authors.initials: S
authors.lastname: Novikoff
authors.forename: Alfredo M
authors.initials: AM
authors.lastname: Goes
authors.forename: Alexandra P
authors.initials: AP
authors.lastname: Marques
authors.forename: Vitor M
authors.initials: VM
authors.lastname: Correlo
authors.forename: Rui L
authors.initials: RL
authors.lastname: Reis
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002450:Cell Communication / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D020405:Dermis / Q000166:cytology / Q000187:drug effects; D004817:Epidermis / Q000187:drug effects* / Q000502:physiology*; D005612:Freeze Drying; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects; D019394:Ki-67 Antigen / Q000378:metabolism; D008422:Materials Testing; D011091:Polyesters / Q000494:pharmacology*; D012997:Solvents; D013718:Tensile Strength / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods*; D014867:Water / Q000737:chemistry; D000094122:Polyhydroxybutyrates
chemical_list: D015415:Biomarkers; D019394:Ki-67 Antigen; D011091:Polyesters; D012997:Solvents; D014867:Water; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates
keywords: bioengineering; biomimetic; in vitro epidermal rearrangement; polyesters; wound healing