title: Repeated ovarian stimulation does not affect the expression level of proteins involved in cell cycle control in mouse ovaries and fallopian tubes.
abstract: OBJECTIVE
  To understand if repeated cycles (2-4 rounds) of gonadotropin stimulation could affect intracellular localization/content of proteins controlling cell cycle progression in mouse fallopian tubes (FT) and ovaries.
  
  
  METHODS
  FT and ovaries of estrous mice (control) and of stimulated mice were analyzed to detect Oct-3/4, Sox-2, p53, β-catenin, pAKT and cyclin D1 localization/content. Spindles and chromosome alignment were analyzed in ovulated oocytes.
  
  
  RESULTS
  After round 4, FT and ovaries of control and stimulated groups showed no differences in Oct-3/4, Sox-2 and β-catenin localization nor in Oct-3/4, Sox-2, p53, β-catenin and pAKT contents. Cyclin D1 level increased significantly in FT of treated mice. Oocytes number decreased meanwhile frequency of abnormal meiotic spindles increased with treatments.
  
  
  CONCLUSIONS
  Repetitive stimulations affected oocyte spindle morphology but did not induce changes in a set of proteins involved in cell cycle progression, usually altered in ovarian cancer. The significant increase of cyclin D1 in the FT requires further investigation.
authors.affiliation: Department of Life, Health and Environmental Sciences, University of L'Aquila, L'Aquila, Italy.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Di Luigi
authors.forename: Gianna
authors.initials: G
authors.lastname: Rossi
authors.forename: Annalisa
authors.initials: A
authors.lastname: Castellucci
authors.forename: Pietro
authors.initials: P
authors.lastname: Leocata
authors.forename: Gaspare
authors.initials: G
authors.lastname: Carta
authors.forename: Rita
authors.initials: R
authors.lastname: Canipari
authors.forename: Stefania Annarita
authors.initials: SA
authors.lastname: Nottola
authors.forename: Sandra
authors.initials: S
authors.lastname: Cecconi
mesh_terms: D000818:Animals; D059447:Cell Cycle Checkpoints / Q000235:genetics*; D005187:Fallopian Tubes / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006062:Gonadotropins / Q000008:administration & dosage; D051379:Mice; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D010062:Ovulation Induction
chemical_list: D006062:Gonadotropins