title: Comparative proteomic analysis of differential responses of Pinus massoniana and Taxus wallichiana var. mairei to simulated acid rain.
abstract: Acid rain (AR), a serious environmental issue, severely affects plant growth and development. As the gymnosperms of conifer woody plants, Pinus massoniana (AR-sensitive) and Taxus wallichiana var. mairei (AR-resistant) are widely distributed in southern China. Under AR stress, significant necrosis and collapsed lesions were found in P. massoniana needles with remarkable yellowing and wilting tips, whereas T. wallichiana var. mairei did not exhibit chlorosis and visible damage. Due to the activation of a large number of stress-related genes and the synthesis of various functional proteins to counteract AR stress, it is important to study the differences in AR-tolerance mechanisms by comparative proteomic analysis of tolerant and sensitive species. This study revealed a total of 65 and 26 differentially expressed proteins that were identified in P. massoniana and T. wallichiana var. mairei, respectively. Among them, proteins involved in metabolism, photosynthesis, signal transduction and transcription were drastically down-regulated in P. massoniana, whereas most of the proteins participating in metabolism, cell structure, photosynthesis and transcription were increased in T. wallichiana var. mairei. These results suggest the distinct patterns of protein expression in the two woody species in response to AR, allowing a deeper understanding of diversity on AR tolerance in forest tree species.
authors.affiliation: Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen 361005, Fujian, China. huwenjun@stu.xmu.edu.cn.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Hu
authors.affiliation: Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen 361005, Fujian, China. chenjuanmn@xmu.edu.cn.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen 361005, Fujian, China. liutw@xmu.edu.cn.
authors.forename: Ting-Wu
authors.initials: TW
authors.lastname: Liu
authors.affiliation: Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen 361005, Fujian, China. martinsimon23@gmail.com.
authors.forename: Martin
authors.initials: M
authors.lastname: Simon
authors.affiliation: Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen 361005, Fujian, China. wangwenhua107@xmu.edu.cn.
authors.forename: Wen-Hua
authors.initials: WH
authors.lastname: Wang
authors.affiliation: Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen 361005, Fujian, China. chenjuanmn@xmu.edu.cn.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen 361005, Fujian, China. wufeihua@xmu.edu.cn.
authors.forename: Fei-Hua
authors.initials: FH
authors.lastname: Wu
authors.affiliation: Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen 361005, Fujian, China. lx19890818@stu.xmu.edu.cn.
authors.forename: Xiang
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen 361005, Fujian, China. shenzj456@sina.com.
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Shen
authors.affiliation: Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen 361005, Fujian, China. zhenghl@xmu.edu.cn.
authors.forename: Hai-Lei
authors.initials: HL
authors.lastname: Zheng
mesh_terms: D015258:Acid Rain; D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D017753:Ecosystem; D015180:Electrophoresis, Gel, Two-Dimensional; D008954:Models, Biological; D008969:Molecular Sequence Data; D010641:Phenotype; D028223:Pinus / Q000033:anatomy & histology / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves / Q000033:anatomy & histology / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D013045:Species Specificity; D013312:Stress, Physiological; D020946:Taxus / Q000033:anatomy & histology / Q000235:genetics / Q000378:metabolism*
chemical_list: D015258:Acid Rain; D010940:Plant Proteins