title: Evaluation of reduced allergenicity of deamidated gliadin in a mouse model of wheat-gliadin allergy using an antibody prepared by a peptide containing three epitopes.
abstract: Gliadin is the principal allergen of wheat-dependent exercise-induced anaphylaxis (WDEIA). The primary structure of IgE-binding epitopes in wheat gliadin includes tandem sequencing sites of glutamine residues. Therefore, deamidation would be an effective approach to reduce the allergenicity of wheat proteins. In our previous study, we deamidated wheat gliadin without causing peptide-bond hydrolysis or polymerization by use of carboxylated cation-exchange resins, and we found that the deamidated gliadin scarcely reacted with the sera of patients radioallergosorbent test (RAST)-positive to wheat. In this study, we examined the allergenicity of deamidated gliadin in a mouse model of wheat-gliadin allergy. Oral administration of deamidated gliadin to gliadin-sensitized mice suppressed enhancement in intestinal permeability, serum allergen level, serum allergen-specific IgE level, mast-cell-surface expression of FcεRI, and serum and intestinal histamine levels. Our results indicate that gliadin deamidated with no peptide-bond hydrolysis by cation-exchange resins has low allergenicity even under in vivo conditions.
authors.affiliation: Department of Chemistry and Life Science, Nihon University , 1866 Kameino, Fujisawa-shi 252-0880, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Abe
authors.forename: Shiori
authors.initials: S
authors.lastname: Shimizu
authors.forename: Karin
authors.initials: K
authors.lastname: Yasuda
authors.forename: Masae
authors.initials: M
authors.lastname: Sugai
authors.forename: Yohei
authors.initials: Y
authors.lastname: Okada
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Chiba
authors.forename: Makoto
authors.initials: M
authors.lastname: Akao
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Kumagai
authors.forename: Hitomi
authors.initials: H
authors.lastname: Kumagai
mesh_terms: D000707:Anaphylaxis / Q000276:immunology / Q000517:prevention & control*; D000818:Animals; D003641:Deamination; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology*; D005903:Gliadin / Q000737:chemistry* / Q000276:immunology*; D006801:Humans; D006868:Hydrolysis; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D007422:Intestines / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D014908:Triticum / Q000737:chemistry / Q000276:immunology*; D021182:Wheat Hypersensitivity / Q000276:immunology / Q000517:prevention & control*
chemical_list: D000939:Epitopes; D007073:Immunoglobulin E; D005903:Gliadin