title: Lateral flow immunoassay for quantitative detection of ractopamine in swine urine.
abstract: A strip reader based lateral flow immunoassay (LFIA) was established for the rapid and quantitative detection of ractopamine (RAC) in swine urine. The ratio of the optical densities (ODs) of the test line (AT) to that of the control line (AC) was used to effectively minimize interference among strips and sample variations. The linear range for the quantitative detection of RAC was 0.2 ng/mL to 3.5 ng/mL with a median inhibitory concentration (IC50) of 0.59 ± 0.06 ng/mL. The limit of detection (LOD) of the LFIA was 0.13 ng/mL. The intra-assay recovery rates were 92.97%, 97.25%, and 107.41%, whereas the inter-assay rates were 80.07%, 108.17%, and 93.7%, respectively.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, Jiangxi, China.
authors.forename: Mei Ling
authors.initials: ML
authors.lastname: Ren
authors.affiliation: Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Jiangxi Normal University, Nanchang 330022, Jiangxi, China.
authors.forename: Xue Lan
authors.initials: XL
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, Jiangxi, China.
authors.forename: Chao Hui
authors.initials: CH
authors.lastname: Li
authors.affiliation: Wuxi Zodoboer Biotech. Co., Ltd., Wuxi 214174, Jiangsu, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Xu
authors.affiliation: Wuxi Zodoboer Biotech. Co., Ltd., Wuxi 214174, Jiangsu, China.
authors.forename: Wen Juan
authors.initials: WJ
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, Jiangxi, China.
authors.forename: Heng Yi
authors.initials: HY
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, Jiangxi, China.
authors.forename: Yong Hua
authors.initials: YH
authors.lastname: Xiong
mesh_terms: D000818:Animals; D007118:Immunoassay; D010627:Phenethylamines / Q000652:urine*; D011934:Reagent Strips; D013552:Swine / Q000652:urine
chemical_list: D010627:Phenethylamines; D011934:Reagent Strips; C060897:ractopamine