title: Involvement of tumor-associated macrophage activation in vitro during development of a novel mantle cell lymphoma cell line, PF-1, derived from a typical patient with relapsed disease.
abstract: Human mantle cell lymphoma (MCL) cell lines are scarce and have been only sporadically described and validated, and only a few have been thoroughly molecularly or genetically characterized. We describe here the successful establishment of a new MCL line, PF-1, with typical MCL characteristics. Culturing primary MCL cells in vitro initially gave rise to an essential generative microenvironment "niche" involving macrophages required for MCL growth, and eventually produced the PF-1 MCL cell line. Our analysis revealed that PF-1 is morphologically and genotypically nearly identical to the original tumor cells. The PF-1 MCL cell line that we have developed will be useful for in vitro and in vivo studies of MCL pathogenesis and therapeutics.
authors.affiliation: Department of Hematopathology, The University of Texas M. D. Anderson Cancer Center , Houston, TX , USA.
authors.forename: Lan V
authors.initials: LV
authors.lastname: Pham
authors.forename: Muychi T
authors.initials: MT
authors.lastname: Vang
authors.forename: Archito T
authors.initials: AT
authors.lastname: Tamayo
authors.forename: Gary
authors.initials: G
authors.lastname: Lu
authors.forename: Pramoda
authors.initials: P
authors.lastname: Challagundla
authors.forename: Jeffrey L
authors.initials: JL
authors.lastname: Jorgensen
authors.forename: Alex A
authors.initials: AA
authors.lastname: Rollo
authors.forename: Zhishuo
authors.initials: Z
authors.lastname: Ou
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.forename: Michael
authors.initials: M
authors.lastname: Wang
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Ford
mesh_terms: D000954:Antigens, Surface / Q000378:metabolism; D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D020522:Lymphoma, Mantle-Cell / Q000235:genetics / Q000276:immunology* / Q000473:pathology*; D008262:Macrophage Activation / Q000276:immunology*; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D009364:Neoplasm Recurrence, Local
chemical_list: D000954:Antigens, Surface
keywords: Mantle cell lymphoma cell line; in vitro microenvironment; macrophages