title: Binding sites of mosquitocidal toxins of Pseudomonas fluorescens and Bacillus subtilis on pupae and larvae of Culex quinquefasciatus.
abstract: Two of the potential bacterial isolates, viz., Pseudomonas fluorescens (VCRC B-426) and Bacillus subtilis (VCRC B-471) whose toxins kill the mosquito pupae/larvae have been identified at our center. As the mode of action of these bacteria are not known, an attempt was made to find out the binding sites of the toxic proteins through immunological methods. Antibodies were raised in BALB/c mice and egg yolk system of chicken layers against the mosquitocidal proteins. The antibodies showed specific binding on to the cephalic and thoracic cuticle of the pupae as well as the paddles of the larvae, indicating the binding of the mosquitocidal proteins.
authors.affiliation: a Unit of Microbiology and Immunology, Vector Control Research Centre (Indian Council of Medical Research), Indira Nagar , Puducherry , India.
authors.forename: K Athisaya
authors.initials: KA
authors.lastname: Mary
authors.forename: K P
authors.initials: KP
authors.lastname: Paily
authors.forename: S L
authors.initials: SL
authors.lastname: Hoti
authors.forename: K
authors.initials: K
authors.lastname: Balaraman
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D001412:Bacillus subtilis / Q000737:chemistry*; D001427:Bacterial Toxins / Q000737:chemistry / Q000494:pharmacology*; D001665:Binding Sites / Q000187:drug effects; D002645:Chickens; D003465:Culex / Q000187:drug effects*; D007814:Larva / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D011551:Pseudomonas fluorescens / Q000737:chemistry*; D011679:Pupa / Q000187:drug effects
chemical_list: D000906:Antibodies; D001427:Bacterial Toxins
keywords: bacillus subtilis; binding sites; egg yolk antibodies; mosquitocidal proteins; pseudomonas fluorescens