title: Detecting apoptosis in Drosophila tissues and cells.
abstract: In this chapter we discuss methods that can be used to study apoptotic cell death in the Drosophila embryo, ovary, as well as in cultured cell lines. These methods assay various aspects of the cell death process, from mitochondrial changes to caspase activation and DNA cleavage. The assays are useful for examining apoptosis in normal development and in response to developmental perturbations and external stresses. These techniques include Acridine Orange staining, TUNEL, cleaved caspase staining, caspase activity assays and assays for mitochondrial fission and permeabilization.
authors.affiliation: CBRC, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02129, USA.
authors.forename: Tatevik
authors.initials: T
authors.lastname: Sarkissian
authors.affiliation: Department of Biology, Boston University, Boston, MA 02215, USA.
authors.forename: Allison
authors.initials: A
authors.lastname: Timmons
authors.affiliation: CBRC, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02129, USA.
authors.forename: Richa
authors.initials: R
authors.lastname: Arya
authors.affiliation: CBRC, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02129, USA.
authors.forename: Eltyeb
authors.initials: E
authors.lastname: Abdelwahid
authors.affiliation: CBRC, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02129, USA. Electronic address: kristin.white@cbrc2.mgh.harvard.edu.
authors.forename: Kristin
authors.initials: K
authors.lastname: White
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D020169:Caspases / Q000378:metabolism; D015509:Developmental Biology / Q000379:methods*; D004330:Drosophila / Q000196:embryology / Q000235:genetics*; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D010539:Permeability
chemical_list: D020169:Caspases
keywords: Apoptosis; Caspase; Drosophila; TUNEL