title: Integration and Expression of gfp in the plastid of Medicago sativa L.
abstract: Here we describe a protocol of alfalfa (Medicago sativa L.) plastid transformation by which gfp, a gene encoding the green fluorescent protein (GFP), is inserted into plastid genome via particle bombardment and homoplastomic plant is obtained. Plastid engineering is likely to make a significant contribution to the genetic improvement of this crop and the production of vaccines and therapeutic proteins.
authors.affiliation: Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, Changchun, P. R. China.
authors.forename: Shaochen
authors.initials: S
authors.lastname: Xing
authors.forename: Zhengyi
authors.initials: Z
authors.lastname: Wei
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Wang
authors.forename: Yanzhi
authors.initials: Y
authors.lastname: Liu
authors.forename: Chunjing
authors.initials: C
authors.lastname: Lin
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D019470:Biolistics / Q000379:methods*; D004351:Drug Resistance / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics*; D000455:Medicago sativa / Q000235:genetics*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009713:Nucleotidyltransferases / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics; D018087:Plastids / Q000235:genetics*; D000198:Spectinomycin / Q000494:pharmacology; D014170:Transformation, Genetic
chemical_list: D000900:Anti-Bacterial Agents; D049452:Green Fluorescent Proteins; D000198:Spectinomycin; D009713:Nucleotidyltransferases; C020565:gentamicin 2''-nucleotidyltransferase