title: Identification of NaCl and NaHCO3 stress responsive proteins in tomato roots using iTRAQ-based analysis.
abstract: Soil salinity and alkalinity are common constraints to crop productivity in low rainfall regions of the world. However, the physiological difference of plant response to these two stresses was short of deep investigation. This study has identified a set of differentially expressed proteins of tomato root exploring to NaCl and NaHCO3 stress by iTRAQ (isobaric tags for relative and absolute quantitation) assay. A total of 313 proteins responsive to NaCl and NaHCO3 were observed. Among these proteins, 70 and 114 proteins were up-regulated by salt and alkali stress, respectively. While down-regulated proteins were 80 in salt treatment and 83 in alkali treatment. Only 39 up-regulated proteins and 30 down-regulated proteins were shared by salt and alkali stresses. The majority of the down-regulated proteins accounted for metabolism and energy conversion, and the up-regulated proteins were involved in signaling or transport. Compared with salt stress, alkali stress down-regulated proteins related with the respiratory metabolism, fatty acid oxidative metabolism and nitrogenous metabolism of tomato roots, and up-regulated protein with the reactive oxygen species (ROS) scavenging and ion transport. This study provides a novel insight into tomato roots response to salt and alkali stress at a large translation level.
authors.affiliation: State Key Laboratory of Crop Biology, PR China; Scientific Observing and Experimental Station of Environment Controlled Agricultural Engineering in Huang-Huai-Hai Region, Ministry of Agriculture, PR China; College of Horticulture Science and Engineering, Shandong Agricultural University, Taian 271018, PR China.
authors.forename: Biao
authors.initials: B
authors.lastname: Gong
authors.affiliation: State Key Laboratory of Crop Biology, PR China; Scientific Observing and Experimental Station of Environment Controlled Agricultural Engineering in Huang-Huai-Hai Region, Ministry of Agriculture, PR China; College of Horticulture Science and Engineering, Shandong Agricultural University, Taian 271018, PR China.
authors.forename: Cunjia
authors.initials: C
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Biology, PR China; Scientific Observing and Experimental Station of Environment Controlled Agricultural Engineering in Huang-Huai-Hai Region, Ministry of Agriculture, PR China; College of Horticulture Science and Engineering, Shandong Agricultural University, Taian 271018, PR China.
authors.forename: Xiu
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Crop Biology, PR China; Scientific Observing and Experimental Station of Environment Controlled Agricultural Engineering in Huang-Huai-Hai Region, Ministry of Agriculture, PR China; College of Horticulture Science and Engineering, Shandong Agricultural University, Taian 271018, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wen
authors.affiliation: State Key Laboratory of Crop Biology, PR China; Scientific Observing and Experimental Station of Environment Controlled Agricultural Engineering in Huang-Huai-Hai Region, Ministry of Agriculture, PR China; College of Horticulture Science and Engineering, Shandong Agricultural University, Taian 271018, PR China.
authors.forename: Shuoshuo
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Biology, PR China; Scientific Observing and Experimental Station of Environment Controlled Agricultural Engineering in Huang-Huai-Hai Region, Ministry of Agriculture, PR China; College of Horticulture Science and Engineering, Shandong Agricultural University, Taian 271018, PR China. Electronic address: qhshi@sdau.edu.cn.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Crop Biology, PR China; Scientific Observing and Experimental Station of Environment Controlled Agricultural Engineering in Huang-Huai-Hai Region, Ministry of Agriculture, PR China; College of Horticulture Science and Engineering, Shandong Agricultural University, Taian 271018, PR China. Electronic address: xfwang@sdau.edu.cn.
authors.forename: Xiufeng
authors.initials: X
authors.lastname: Wang
mesh_terms: D002352:Carrier Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D015536:Down-Regulation; D018506:Gene Expression Regulation, Plant; D006360:Heat-Shock Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism*; D009997:Osmotic Pressure; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism; D020543:Proteome / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D017382:Reactive Oxygen Species / Q000378:metabolism; D054712:Salinity; D055049:Salt Tolerance; D015398:Signal Transduction; D017693:Sodium Bicarbonate; D012987:Soil / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013312:Stress, Physiological; D053719:Tandem Mass Spectrometry; D015854:Up-Regulation
chemical_list: D002352:Carrier Proteins; D006360:Heat-Shock Proteins; D010940:Plant Proteins; D020543:Proteome; D017382:Reactive Oxygen Species; D012987:Soil; D017693:Sodium Bicarbonate
keywords: Alkali stress; Proteomics; Salt stress; Tomato