title: Modulation of Strawberry/Cranberry Phenolic Compounds Glucuronidation by Co-Supplementation with Onion: Characterization of Phenolic Metabolites in Rat Plasma Using an Optimized μSPE-UHPLC-MS/MS Method.
abstract: Plant phenolic compounds are suggested to exert pharmacological activities in regards to obesity and type-2 diabetes, but their mode of action is poorly understood due to a lack of information about their bioavailability. This work aimed to study the bioavailability of GlucoPhenol phenolic compounds, a strawberry-cranberry extracts blend, by characterizing plasma phenolic profile in obese rats. A comparison was performed by co-supplementation with an onion extract. Using an optimized μSPE-UHPLC-MS/MS method, 21 phenolic metabolites were characterized, mostly conjugated metabolites and microbial degradation products of the native phenolic compounds. Their kinetic profiles revealed either an intestinal or hepatic formation. Among identified metabolites, isorhamnetin glucuronide sulfate was found in greater amount in plasma. Three glucuronidated conjugates of strawberry-cranberry phenolic compounds, p-hydroxybenzoic acid glucuronide, catechins glucuronide, and methyl catechins glucuronide were found in higher quantities when GlucoPhenol was ingested together with onion extract (+252%, +279%, and +118% respectively), suggesting a possible induction of glucuronidation processes by quercetin. This work allowed the characterization of actual phenolic metabolites generated in vivo following a phenolic intake, the analysis of their kinetics and suggested a possible synergistic activity of phenolic compounds for improving bioavailability.
authors.affiliation: Institute of Nutrition and Functional Foods (INAF), Laval University , 2440 Boulevard Hochelaga, Québec (QC) G1V 0A6, Canada.|Research Center of Horticulture (CRH), Laval University , 2480 Boulevard Hochelaga, Québec (QC) G1V 0A6, Canada.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Dudonné
authors.affiliation: Institute of Nutrition and Functional Foods (INAF), Laval University , 2440 Boulevard Hochelaga, Québec (QC) G1V 0A6, Canada.|Research Center of Horticulture (CRH), Laval University , 2480 Boulevard Hochelaga, Québec (QC) G1V 0A6, Canada.
authors.forename: Pascal
authors.initials: P
authors.lastname: Dubé
authors.affiliation: Institute of Nutrition and Functional Foods (INAF), Laval University , 2440 Boulevard Hochelaga, Québec (QC) G1V 0A6, Canada.|Department of Medicine, Quebec Heart and Lung Institute (CRIUCPQ), Laval University , 2725 Chemin Ste-Foy, Québec (QC) G1V 4G5, Canada.
authors.forename: Geneviève
authors.initials: G
authors.lastname: Pilon
authors.affiliation: Institute of Nutrition and Functional Foods (INAF), Laval University , 2440 Boulevard Hochelaga, Québec (QC) G1V 0A6, Canada.|Department of Medicine, Quebec Heart and Lung Institute (CRIUCPQ), Laval University , 2725 Chemin Ste-Foy, Québec (QC) G1V 4G5, Canada.
authors.forename: André
authors.initials: A
authors.lastname: Marette
authors.affiliation: Institute of Nutrition and Functional Foods (INAF), Laval University , 2440 Boulevard Hochelaga, Québec (QC) G1V 0A6, Canada.|Department of Food and Nutrition Sciences, Laval University , 2425 Rue de l'Agriculture, Québec (QC) G1V 0A6, Canada.
authors.forename: Hélène
authors.initials: H
authors.lastname: Jacques
authors.affiliation: Laval University Hospital Center (CHUL) , Department of medicine, 2705 Boulevard Laurier, Québec (QC) G1V 4G2, Canada.
authors.forename: John
authors.initials: J
authors.lastname: Weisnagel
authors.affiliation: Institute of Nutrition and Functional Foods (INAF), Laval University , 2440 Boulevard Hochelaga, Québec (QC) G1V 0A6, Canada.|Research Center of Horticulture (CRH), Laval University , 2480 Boulevard Hochelaga, Québec (QC) G1V 0A6, Canada.
authors.forename: Yves
authors.initials: Y
authors.lastname: Desjardins
keywords: GlucoPhenol; UHPLC-MS/MS; bioavailability; phenolic metabolites; μSPE