title: Characterization of Gp41 polymorphisms in the fusion peptide domain and T-20 (Enfuvirtide) resistance-associated regions in Korean HIV-1 isolates.
abstract: HIV-1 gp41 is an envelope protein that plays an essential role in virus entry. The mutation of gp41 affects HIV-1 entry and susceptibility to the fusion inhibitor T-20. Therefore, we analyzed the natural polymorphism of gp41 of 163 HIV-1 isolates from T-20-naïve Koreans infected with HIV-1. This study of gp41 polymorphisms showed that insertions in the fourth threonine (74.8%) and L7M substitutions (85.3%) were more frequent in the fusion peptide motif in Korean HIV-1 isolates compared with those from other countries. Minor T-20 resistance mutations such as L45M (1.2%), N126K (1.2%), and E137K (6.7%) were detected, but the critical T-20 resistance mutations were not detected in the gp41 HR1 and HR2 regions. In addition, the N42S mutation (12.9%) associated with T-20 hypersusceptibility was detected at a high frequency. These results may serve as useful data for studies considering T-20 for use in the development of a more effective anti-retroviral treatment in Korea.
authors.affiliation: Division of AIDS, Korea National Institute of Health, Cheongwon, Korea. ; School of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
authors.forename: Dai-Ho
authors.identifier: 0000-0002-0691-9857
authors.initials: DH
authors.lastname: Jang
authors.affiliation: Division of AIDS, Korea National Institute of Health, Cheongwon, Korea.
authors.forename: Cheol-Hee
authors.identifier: 0000-0002-5696-0567
authors.initials: CH
authors.lastname: Yoon
authors.affiliation: Division of AIDS, Korea National Institute of Health, Cheongwon, Korea.
authors.forename: Byeong-Sun
authors.identifier: 0000-0002-6128-7995
authors.initials: BS
authors.lastname: Choi
authors.affiliation: Division of AIDS, Korea National Institute of Health, Cheongwon, Korea.
authors.forename: Yoon-Seok
authors.identifier: 0000-0003-4562-5533
authors.initials: YS
authors.lastname: Chung
authors.affiliation: Division of AIDS, Korea National Institute of Health, Cheongwon, Korea.
authors.forename: Hye-Young
authors.identifier: 0000-0001-9664-2179
authors.initials: HY
authors.lastname: Kim
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
authors.forename: Sung-Gil
authors.identifier: 0000-0003-4643-9003
authors.initials: SG
authors.lastname: Chi
authors.affiliation: Division of AIDS, Korea National Institute of Health, Cheongwon, Korea.
authors.forename: Sung Soon
authors.identifier: 0000-0003-2774-6710
authors.initials: SS
authors.lastname: Kim
mesh_terms: D019380:Anti-HIV Agents / Q000494:pharmacology; D024882:Drug Resistance, Viral / Q000235:genetics*; D000077560:Enfuvirtide; D015700:HIV Envelope Protein gp41 / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D015658:HIV Infections / Q000821:virology; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D010446:Peptide Fragments / Q000494:pharmacology; D011110:Polymorphism, Genetic; D017434:Protein Structure, Tertiary / Q000235:genetics; D056910:Republic of Korea; D053586:Virus Internalization
chemical_list: D019380:Anti-HIV Agents; D015700:HIV Envelope Protein gp41; D010446:Peptide Fragments; C514787:gp41 protein, Human immunodeficiency virus 1; D000077560:Enfuvirtide
keywords: Fusion Inhibitor; Fusion Peptide; Gp41 Polymorphism; HIV-1 Envelope Glycoprotein; T-20 Resistance Mutation