title: [Features of population composition of peripheral blood CXCR3-positive lymphocytes in chronic viral hepatitis c patients].
abstract: OBJECTIVE
  Quantitative determination ofCXCR3+, CCR5+ and CCR6+ cells in major lymphocyte populations: T-helpers (Th), cytotoxic T-lymphocytes (CTL), natural killers (NK) and T-natural killer cells (TNK), B-lymphocytes in patients with chronic viral hepatitis C (CVHC).
  
  
  METHODS
  Content of lymphocyte populations carrying chemokine receptor CXCR3 was studied, chemokine receptors CCR5 and CCR6 were evaluated on T-lymphocytes, in peripheral blood of 19 CVHC patients and 32 conditionally healthy donors. Cell populations were determined by flow cytofluorometry by using various combinations of monoclonal antibodies: for evaluation of Th and CTL (CD3/CD4/CD8/CXCR3/CCR5/CCR6); NK and TNK (CDl6/CD56/CD3/ CXCR3); B-cells (CD 19/CD45/CXCR3).
  
  
  RESULTS
  In patients with CVHV compared with healthy donors a significant increase of quantity of CXCR3-positive Th was detected, however the content of CXCR3-positive CTL did not differ in the groups compared; CXCR3+ NK cell content was lower with equal content of CXCR3+ TNK. Analysis of quantity of CXCR3+ B-cells showed an increase of more than 3.5 times in CVHC patients. Significant differences in relative content of Th and CTL carrying CCR5 and CCR6 were not detected despite a non-significant increase of quantity of CCR5+ and CCR6+ Th.
  
  
  CONCLUSIONS
  Content of major lymphocyte populations carrying chemokine receptor CXCR3 changed significantly compared with conditionally healthy donors in peripheral blood of CVHC patients. The increase of quantity of CXCR3-positive B-cells may be associated with infection of these cells by HCV or development of extra-liver manifestations of HVHC.
authors.forename: A V
authors.initials: AV
authors.lastname: Semenov
authors.forename: N A
authors.initials: NA
authors.lastname: Arsent'eva
authors.forename: D S
authors.initials: DS
authors.lastname: Elezov
authors.forename: I V
authors.initials: IV
authors.lastname: Kudriavtsev
authors.forename: E V
authors.initials: EV
authors.lastname: Ésaulenko
authors.forename: Areg A
authors.initials: AA
authors.lastname: Totolian
mesh_terms: D005260:Female; D019698:Hepatitis C, Chronic / Q000097:blood* / Q000276:immunology / Q000473:pathology; D006801:Humans; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008297:Male; D019713:Receptors, CCR5 / Q000097:blood / Q000276:immunology; D054399:Receptors, CCR6 / Q000097:blood / Q000276:immunology; D054367:Receptors, CXCR3 / Q000097:blood* / Q000276:immunology
chemical_list: C584933:CCR5 protein, human; C106311:CCR6 protein, human; C515187:CXCR3 protein, human; D019713:Receptors, CCR5; D054399:Receptors, CCR6; D054367:Receptors, CXCR3