title: The role and reliability of rapid bedside diagnostic test in early diagnosis and treatment of bacterial meningitis.
abstract: OBJECTIVE
  To evaluate the role and reliability of rapid bedside diagnostic test in early diagnosis and treatment of bacterial meningitis in children using reagent strips.
  
  
  METHODS
  This prospective, single blinded study was conducted in the Department of Pediatrics of VMMC & Safdarjung Hospital, New Delhi in collaboration with the Department of Microbiology of VMMC & Safdarjung Hospital, New Delhi, over a period of 15 mo (August 2009 to Nov 2010). Seventy-five children aged 3 mo to 12 y admitted in the pediatric ward with suspected diagnosis of acute meningitis were included. All enroled patients underwent lumbar puncture. CSF samples were taken and divided in 2 parts for laboratory evaluation and rapid strip analysis. The sensitivity, specificity, positive predictive value and the negative predictive values of the reagent strips for the diagnosis of bacterial meningitis were calculated. Accuracy of the reagent strips was established using kappa statistics. Latex agglutination for antigen detection and microbiological culture were also done.
  
  
  RESULTS
  Highly significant association was observed between CSF examination in routine laboratory method and dipstick method. The number of laboratory values that correlated were- for cells 71(94.63%), for protein 68 (90.67%), for glucose 68(90.67%) out of total 75 cases. The sensitivity and specificity of reagent strip in diagnosing acute bacterial meningitis were 96.7% and 97.8% respectively. The positive predictive and negative predictive values of reagent strip in diagnosing acute bacterial meningitis were 96.7% and 97.8% respectively. Staphylococcus aureus was found to be the most common organism isolated (50%).
  
  
  CONCLUSIONS
  Thus reagent strip analysis is a very rapid, reliable and effective method for diagnosis of acute bacterial meningitis in children. Staphylococcus aureus was the most common organism isolated.
authors.affiliation: Department of Pediatrics, Vardhman Mahavir Medical College and Safdarjang Hospital, New Delhi, 110029, India.
authors.forename: Arun
authors.initials: A
authors.lastname: Kumar
authors.forename: Pradeep Kumar
authors.initials: PK
authors.lastname: Debata
authors.forename: Amitabh
authors.initials: A
authors.lastname: Ranjan
authors.forename: Rajani
authors.initials: R
authors.lastname: Gaind
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification; D002648:Child; D002675:Child, Preschool; D061827:Dimensional Measurement Accuracy; D042241:Early Diagnosis; D005260:Female; D006801:Humans; D007194:India; D007223:Infant; D008297:Male; D016920:Meningitis, Bacterial / Q000134:cerebrospinal fluid / Q000175:diagnosis / Q000382:microbiology; D000067716:Point-of-Care Testing / Q000706:statistics & numerical data*; D011446:Prospective Studies; D011934:Reagent Strips / Q000494:pharmacology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013129:Spinal Puncture / Q000379:methods / Q000706:statistics & numerical data
chemical_list: D011934:Reagent Strips