title: Ex vivo bone morphogenetic protein 2 gene delivery using periodontal ligament stem cells for enhanced re-osseointegration in the regenerative treatment of peri-implantitis.
abstract: Peri-implantitis is a chronic inflammatory process with advanced bone loss and impaired healing potential. For peri-implantitis treatment, tissue engineering can be applied to enhance bone regeneration of peri-implant defects. This study aimed to evaluate ex vivo bone morphogenetic protein 2 (BMP2) gene delivery using canine periodontal ligament stem cells (PDLSCs) for regeneration of peri-implantitis defects. Canine PDLSCs were transduced with adenoviral vectors containing BMP2 (BMP2/PDLSCs). After peri-implantitis was induced by ligature placement in six beagle dogs, regenerative procedures were performed; hydroxyapatite (HA) particles and collagen gel with autologous canine PDLSCs (PDLSC group) or BMP2/PDLSCs (BMP/PDLSC group) or without cells (control group) were grafted into the defects and covered by an absorbable membrane. Three months later, the animals were sacrificed. In vitro, BMP2/PDLSCs showed similar levels of stem cell properties to PDLSCs, such as colony-forming efficiency and expression of MSC markers STRO-1 and CD 146. BMP2/PDLSCs produced BMP-2 until day 21 at a concentration of 4-8 ng/mL. In vivo, the BMP2/PDLSC group showed significantly more new bone formation and re-osseointegration in peri-implantitis defects compared to the other groups. In conclusion, ex vivo BMP2 gene delivery using PDLSCs enhanced new bone formation and re-osseointegration in peri-implantitis defects.
authors.affiliation: Department of Periodontology and Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea; Department of Periodontology, Seoul National University Bundang Hospital, Seongnam, Korea.
authors.forename: Shin-Young
authors.initials: SY
authors.lastname: Park
authors.forename: Kyoung-Hwa
authors.initials: KH
authors.lastname: Kim
authors.forename: Eun-Hye
authors.initials: EH
authors.lastname: Gwak
authors.forename: Sang-Hoon
authors.initials: SH
authors.lastname: Rhee
authors.forename: Jeong-Cheol
authors.initials: JC
authors.lastname: Lee
authors.forename: Seung-Yun
authors.initials: SY
authors.lastname: Shin
authors.forename: Ki-Tae
authors.initials: KT
authors.lastname: Koo
authors.forename: Yong-Moo
authors.initials: YM
authors.lastname: Lee
authors.forename: Yang-Jo
authors.initials: YJ
authors.lastname: Seol
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics*; D004285:Dogs; D057873:Peri-Implantitis / Q000628:therapy*; D010513:Periodontal Ligament / Q000166:cytology*; D012038:Regeneration; D013234:Stem Cells / Q000166:cytology*; D014162:Transfection
chemical_list: D055396:Bone Morphogenetic Protein 2
keywords: bone morphogenetic proteins-2; bone regeneration; gene therapy; peri-implantitis; tissue engineering