title: A high-resolution genetic map of yellow monkeyflower identifies chemical defense QTLs and recombination rate variation.
abstract: Genotyping-by-sequencing methods have vastly improved the resolution and accuracy of genetic linkage maps by increasing both the number of marker loci as well as the number of individuals genotyped at these loci. Using restriction-associated DNA sequencing, we construct a dense linkage map for a panel of recombinant inbred lines derived from a cross between divergent ecotypes of Mimulus guttatus. We used this map to estimate recombination rate across the genome and to identify quantitative trait loci for the production of several secondary compounds (PPGs) of the phenylpropanoid pathway implicated in defense against herbivores. Levels of different PPGs are correlated across recombinant inbred lines suggesting joint regulation of the phenylpropanoid pathway. However, the three quantitative trait loci identified in this study each act on a distinct PPG. Finally, we map three putative genomic inversions differentiating the two parental populations, including a previously characterized inversion that contributes to life-history differences between the annual/perennial ecotypes.
authors.affiliation: Department of Entomology, University of Wisconsin, Madison, Wisconsin.
authors.forename: Liza M
authors.initials: LM
authors.lastname: Holeski
authors.affiliation: Department of Ecology and Evolutionary Biology, University of Kansas, Lawrence, Kansas.
authors.forename: Patrick
authors.initials: P
authors.lastname: Monnahan
authors.affiliation: Department of Ecology and Evolutionary Biology, University of Kansas, Lawrence, Kansas.
authors.forename: Boryana
authors.initials: B
authors.lastname: Koseva
authors.affiliation: Department of Ecology and Evolutionary Biology, University of Kansas, Lawrence, Kansas.
authors.forename: Nick
authors.initials: N
authors.lastname: McCool
authors.affiliation: Department of Entomology, University of Wisconsin, Madison, Wisconsin.
authors.forename: Richard L
authors.initials: RL
authors.lastname: Lindroth
authors.affiliation: Department of Ecology and Evolutionary Biology, University of Kansas, Lawrence, Kansas jkk@ku.edu.
authors.forename: John K
authors.initials: JK
authors.lastname: Kelly
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D008040:Genetic Linkage; D005838:Genotype; D007178:Inbreeding; D032241:Mimulus / Q000235:genetics*; D010641:Phenotype; D040641:Quantitative Trait Loci; D019655:Quantitative Trait, Heritable; D011995:Recombination, Genetic
keywords: MSG; QTL; inversions; mimulus; recombination