title: Drinking water biofilm cohesiveness changes under chlorination or hydrodynamic stress.
abstract: Attempts at removal of drinking water biofilms rely on various preventive and curative strategies such as nutrient reduction in drinking water, disinfection or water flushing, which have demonstrated limited efficiency. The main reason for these failures is the cohesiveness of the biofilm driven by the physico-chemical properties of its exopolymeric matrix (EPS). Effective cleaning procedures should break up the matrix and/or change the elastic properties of bacterial biofilms. The aim of this study was to evaluate the change in the cohesive strength of two-month-old drinking water biofilms under increasing hydrodynamic shear stress τw (from ∼0.2 to ∼10 Pa) and shock chlorination (applied concentration at T0: 10 mg Cl2/L; 60 min contact time). Biofilm erosion (cell loss per unit surface area) and cohesiveness (changes in the detachment shear stress and cluster volumes measured by atomic force microscopy (AFM)) were studied. When rapidly increasing the hydrodynamic constraint, biofilm removal was found to be dependent on a dual process of erosion and coalescence of the biofilm clusters. Indeed, 56% of the biofilm cells were removed with, concomitantly, a decrease in the number of the 50-300 μm(3) clusters and an increase in the number of the smaller (i.e., <50 μm(3)) and larger (i.e., >600 μm(3)) ones. Moreover, AFM evidenced the strengthening of the biofilm structure along with the doubling of the number of contact points, NC, per cluster volume unit following the hydrodynamic disturbance. This suggests that the compactness of the biofilm exopolymers increases with hydrodynamic stress. Shock chlorination removed cells (-75%) from the biofilm while reducing the volume of biofilm clusters. Oxidation stress resulted in a decrease in the cohesive strength profile of the remaining drinking water biofilms linked to a reduction in the number of contact points within the biofilm network structure in particular for the largest biofilm cluster volumes (>200 μm(3)). Changes in the cohesive strength of drinking water biofilms subsequent to cleaning/disinfection operations call into question the effectiveness of cleaning-in-place procedures. The combined alternating use of oxidation and shear stress sequences needs to be investigated as it could be an important adjunct to improving biofilm removal/reduction procedures.
authors.affiliation: Ecole Pratique des Hautes Etudes (EPHE), LCPME, UMR 7564 CNRS - Université de Lorraine, Nancy, France. Electronic address: laurence.mathieu@univ-lorraine.fr.
authors.forename: L
authors.initials: L
authors.lastname: Mathieu
authors.affiliation: CNRS and Université de Lorraine, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement (LCPME), UMR 7564, Nancy, France.
authors.forename: I
authors.initials: I
authors.lastname: Bertrand
authors.affiliation: CNRS and Université de Lorraine, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement (LCPME), UMR 7564, Nancy, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Abe
authors.affiliation: Ecole Pratique des Hautes Etudes (EPHE), LCPME, UMR 7564 CNRS - Université de Lorraine, Nancy, France.
authors.forename: E
authors.initials: E
authors.lastname: Angel
authors.affiliation: CNRS and Université de Lorraine, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement (LCPME), UMR 7564, Nancy, France.
authors.forename: J C
authors.initials: JC
authors.lastname: Block
authors.affiliation: CNRS and Université de Lorraine, Laboratoire d'Energétique et de Mécanique Théorique et Appliquée (LEMTA), UMR 7563, Nancy, France.
authors.forename: S
authors.initials: S
authors.lastname: Skali-Lami
authors.affiliation: CNRS and Université de Lorraine, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement (LCPME), UMR 7564, Nancy, France.
authors.forename: G
authors.initials: G
authors.lastname: Francius
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology; D018441:Biofilms / Q000254:growth & development*; D060766:Drinking Water / Q000382:microbiology*; D057446:Hydrodynamics; D018625:Microscopy, Atomic Force; D013314:Stress, Mechanical
chemical_list: D060766:Drinking Water
keywords: AFM; Chlorination; Cohesiveness; Drinking water biofilm; Entanglement rate; Hydrodynamic shear rate