title: [Simultaneous trichothecenes analysis by LC-MS/MS with a pentafluorophenyl column].
abstract: We developed a method for simultaneous analysis of nine trichothecenes (nivalenol, deoxynivalenol, fusarenon-X, neosolaniol, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, diacetoxyscirpenol, HT-2 toxin, and T-2 toxin). Trichothecenes in samples were extracted with acetonitrile-water (1 : 1) and purified using a QuEChERS kit and a multi-functional cartridge. Then they were quantified by LC-MS/MS using internal standards. LC with a pentafluorophenyl column and a mobile phase containing methanol enabled complete separation and highly sensitive quantification of the nine trichothecenes. Method validation with powdered corn soup samples demonstrated high linearity (>0.99) and accuracy ranging from 95% to 111% with good repeatability of 0.9% to 6.6% and reproducibility of 0.6% to 11.6%. The limits of detection and quantification were 0.01 to 0.75 μg/kg and 0.04 to 2.50 μg/kg, respectively. When 15 samples from powdered corn soups on the market were analyzed with this method, deoxynivalenol was detected in two samples (20.7 and 22.5 μg/kg, respectively), and 3-acetyldeoxynivalenol was concomitantly detected in one of them (13.5 μg/kg).
authors.affiliation: Research Laboratories for Food Safety Chemistry.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Tamura
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakagawa
authors.forename: Atsuo
authors.initials: A
authors.lastname: Uyama
authors.forename: Naoki
authors.initials: N
authors.lastname: Mochizuki
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D005464:Fluorobenzenes / Q000737:chemistry*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D005658:Fungi; D008968:Molecular Conformation; D010636:Phenols / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D014255:Trichothecenes / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D005466:Fluorocarbons
chemical_list: D005464:Fluorobenzenes; D010636:Phenols; D014255:Trichothecenes; C085539:2,3,4,5,6-pentafluorophenol; D005466:Fluorocarbons