title: Quantitative colocalization analysis of fluorescence microscopy images.
abstract: Colocalization is an important finding in many cell biological studies. This unit describes a protocol for quantitative evaluation of fluorescence microscopy images with colocalization based on calculation of a number of specialized coefficients. Images of double-stained sections are first subjected to background correction, and then various coefficients are calculated. Meanings of the coefficients and a guide to interpretation of the results of calculations based on the use of linguistic variables are given. Success in colocalization studies depends on the quality of images analyzed, proper preparation of the images for coefficients calculations, and correct interpretation of results obtained. This protocol helps ensure reliability of colocalization coefficient calculations.
authors.affiliation: Department of Neurobiology and Anatomy, Kochi University, Faculty of Medicine, Japan.
authors.forename: Vadim
authors.initials: V
authors.lastname: Zinchuk
authors.affiliation: Institute of Anatomy, University of Bern, Bern, Switzerland.
authors.forename: Olga
authors.initials: O
authors.lastname: Grossenbacher-Zinchuk
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000032:analysis / Q000378:metabolism; D000074020:ATP Binding Cassette Transporter, Subfamily B, Member 11; D018528:ATP-Binding Cassette Transporters / Q000032:analysis / Q000378:metabolism; D000818:Animals; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods*; D051381:Rats; D012984:Software; D000097202:ATP-Binding Cassette Sub-Family B Member 4
chemical_list: D018435:ATP Binding Cassette Transporter, Subfamily B; D000074020:ATP Binding Cassette Transporter, Subfamily B, Member 11; D018528:ATP-Binding Cassette Transporters; D000097202:ATP-Binding Cassette Sub-Family B Member 4; C490548:Abcb11 protein, rat
keywords: data interpretation; fluorescence microscopy; image analysis; quantitative colocalization