title: Prognostic implications for high expression of MiR-25 in lung adenocarcinomas of female non-smokers.
abstract: BACKGROUND
  Adenocarcinoma (ADC) is the most common histological type of lung cancer and its proportion is rising, especially in Asian non-smoking women. Recent studies suggest miR-25 may have diverse effects on the pathogenesis of different types of cancer. However, the role of miR-25 in lung cancer is still unknown. The aim of this study was to investigate the potential clinical value of miR-25 in non-smoking women with lung ADC.
  
  
  METHODS
  Quantitative RT-PCR was performed to evaluate the expression of miR-25 in 100 lung ADC tumor tissues and matched plasma samples and Pearson correlation tests were used to analyze the relationship between values. Associations of miR-25 expression with clinicopathological features were determined using the Student's t-test. To determine prognostic value, overall survival (OS) was evaluated using the Kaplan-Meier method. Univariate and multivariate analyses were performed using the Cox proportional hazard model.
  
  
  RESULTS
  Expression of miR-25 in tissue was found to be associated with lymph node metastasis (P=0.021) and disease stage (P=0.012). Moreover, high miR-25 expression was also associated with poorer overall survival of women with lung ADC (P=0.008).
  
  
  CONCLUSIONS
  Tissue miR-25 expression may be associated with tumor progression and have prognostic implications in female lung ADC patients.
authors.affiliation: Department of Epidemiology and Biostatistics, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center of Cancer, Tianjin, China E-mail : qianbiyun@126.com.
authors.forename: Fang-Xiu
authors.initials: FX
authors.lastname: Xu
authors.forename: Yu-Liang
authors.initials: YL
authors.lastname: Su
authors.forename: Huan
authors.initials: H
authors.lastname: Zhang
authors.forename: Jin-Yu
authors.initials: JY
authors.lastname: Kong
authors.forename: Herbert
authors.initials: H
authors.lastname: Yu
authors.forename: Bi-Yun
authors.initials: BY
authors.lastname: Qian
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000401:mortality; D000077192:Adenocarcinoma of Lung; D014408:Biomarkers, Tumor / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000401:mortality; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000401:mortality; D008207:Lymphatic Metastasis / Q000235:genetics; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D015999:Multivariate Analysis; D009367:Neoplasm Staging; D012907:Smoking / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; C543351:MIRN25 microRNA, human; D035683:MicroRNAs