title: [Inhibitory effect of 2'-hydroxyflavanone on proliferation, invasion and migration of bladder cancer cells in vitro via blocking AKT/STAT3 signaling pathway].
abstract: OBJECTIVE
  To study the effect of 2'-hydroxyflavanone on proliferation, invasion and migration of bladder cancer cells and its mechanism.
  
  
  METHODS
  MTT assay was used to detect the effect of 2'-hydroxyflavanone on proliferation of bladdercancer cells, and the proliferation inhibition rate was calculated. Wound healing assay and Transwell (TM); assay were conducted to study the effect of 2'-hydroxyflavanone on migration and invasion of bladder cancer cells, respectively. Western blotting was performed to investigate the change of migration-associated proteins after 2'-hydroxyflavanone treatment.
  
  
  RESULTS
  2'-hydroxyflavanone inhibited the proliferation of bladder cancer cells (5637, T24, UMUC-3, 253J) in a time- and dose- dependent manner. The migration and invasion abilities of T24 cells were significantly reduced by 2'-hydroxyflavanone treatment (P<0.05), which was accompanied by the down-regulation of MMP-2, MMP-9, p-AKT and p-STAT3 protein expressions.
  
  
  CONCLUSIONS
  2'-hydroxyflavanone can effectively inhibit bladder cancer cell proliferation, invasion and migration, in which the inhibition of AKT/STAT3 signaling pathway may play an important role.
authors.affiliation: Department of Urology, First Affiliated Hospital, Medical School, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Jianning
authors.initials: J
authors.lastname: Zhu
authors.forename: Kaijie
authors.initials: K
authors.lastname: Wu
authors.forename: Yule
authors.initials: Y
authors.lastname: Chen
authors.forename: Zhongyun
authors.initials: Z
authors.lastname: Ning
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.forename: Yiqing
authors.initials: Y
authors.lastname: DU
authors.forename: Chen
authors.initials: C
authors.lastname: Ding
authors.forename: Xinyang
authors.initials: X
authors.lastname: Wang
authors.forename: Jinhai
authors.initials: J
authors.lastname: Fan
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D044950:Flavanones / Q000494:pharmacology*; D006801:Humans; D009361:Neoplasm Invasiveness; D051057:Proto-Oncogene Proteins c-akt / Q000037:antagonists & inhibitors / Q000502:physiology*; D050796:STAT3 Transcription Factor / Q000037:antagonists & inhibitors / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects*; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy* / Q000473:pathology
chemical_list: C520534:2'-hydroxyflavanone; D044950:Flavanones; D050796:STAT3 Transcription Factor; D051057:Proto-Oncogene Proteins c-akt