title: ATP/ADP alteration as a sign of the oxidative stress development in Escherichia coli cells under antibiotic treatment.
abstract: The extensively discussed idea of oxidative stress development under antibiotic treatment was confirmed using an antioxidant gene expression (soxRS-, oxyR-regulon) approach, including microaerobic cultivation conditions. The killing action of antibiotics and their ability to cause peroxide oxidative stress in Escherichia coli cells was comparable to a similar hydrogen peroxide capacity; therefore, the involvement of intracellular hydrogen peroxide production in the killing action of antibiotics seems plausible under conditions studied. The temporary increase of ATP/ADP (which returned to untreated levels in 10 min) and the intensification of respiration preceded the development of oxidative stress. The sharp rise in ATP/ADP was due to the accumulation of ATP with a slight increase in the ADP content. We proposed that ATP accumulation was not a result of increased respiration but was due to the inhibition of energy-consuming processes. The association of reactive oxygen species formation under antibiotic treatment with the inhibition of direct electron flow pathway along the respiratory chain, and a possible role of a sharp rise in ATP/ADP in this process is hypothesized.
authors.affiliation: Institute of Ecology and Genetics of Microorganisms, Perm, Russia.
authors.forename: Anna V
authors.initials: AV
authors.lastname: Akhova
authors.forename: Alexander G
authors.initials: AG
authors.lastname: Tkachenko
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate
keywords: adenyl nucleotides; antibiotic susceptibility; oxygen consumption; reactive oxygen species; sub-lethal stress