title: Mutation screening of the TP53 gene by temporal temperature gel electrophoresis (TTGE).
abstract: A protocol for detection of mutations in the TP53 gene using temporal temperature gradient electrophoresis (TTGE) is described. TTGE is a mutation detection technique that separates DNA fragments differing by single base pairs according to their melting properties in a denaturing gel. It is based on constant denaturing conditions in the gel combined with a temperature gradient during the electrophoretic run. This method combines some of the advantages of the related techniques, denaturing gradient gel electrophoresis and constant denaturant gel electrophoresis, and eliminates some of the problems. The result is a rapid and sensitive screening technique which is robust and easily set up in smaller laboratory environments.
authors.affiliation: Department of Genetics, Oslo University Hospital The Norwegian Radium Hospital, Institute for Cancer Research, 4953, Nydalen, 0424, Oslo, Norway, tsorlie@uio.no.
authors.forename: Therese
authors.initials: T
authors.lastname: Sørlie
authors.forename: Hilde
authors.initials: H
authors.lastname: Johnsen
authors.forename: Phuong
authors.initials: P
authors.lastname: Vu
authors.forename: Guro Elisabeth
authors.initials: GE
authors.lastname: Lind
authors.forename: Ragnhild
authors.initials: R
authors.lastname: Lothe
authors.forename: Anne-Lise
authors.initials: AL
authors.lastname: Børresen-Dale
mesh_terms: D001483:Base Sequence; D004252:DNA Mutational Analysis; D017931:DNA Primers / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008969:Molecular Sequence Data; D012680:Sensitivity and Specificity; D044366:Transition Temperature; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D017931:DNA Primers; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53