title: Most but not all laboratories can detect the recently emerged Neisseria gonorrhoeae porA mutants - results from the QCMD 2013 N. gonorrhoeae external quality assessment programme.
abstract: We describe the results of the Quality Control for Molecular Diagnostics 2013 Neisseria gonorrhoeae external quality assessment programme that included an N. gonorrhoeae strain harbouring an N. meningitidis porA gene which causes false-negative results in molecular diagnostic assays targeting the gonococcal porA pseudogene. Enhanced awareness of the international transmission of such gonococcal strains is needed to avoid false-negative results in both in-house and commercial molecular diagnostic assays used in laboratories worldwide, but particularly in Europe.
authors.affiliation: Certe, Department of Medical Microbiology, Groningen, the Netherlands.
authors.forename: D
authors.initials: D
authors.lastname: Luijt
authors.forename: C
authors.initials: C
authors.lastname: Di Lorenzo
authors.forename: A M
authors.initials: AM
authors.lastname: van Loon
authors.forename: M
authors.initials: M
authors.lastname: Unemo
mesh_terms: D005060:Europe; D005188:False Negative Reactions; D014644:Genetic Variation; D006069:Gonorrhea / Q000175:diagnosis* / Q000235:genetics / Q000382:microbiology; D006801:Humans; D059021:Laboratory Proficiency Testing; D008969:Molecular Sequence Data; D058889:Molecular Typing; D009154:Mutation; D009344:Neisseria gonorrhoeae / Q000235:genetics* / Q000302:isolation & purification; D009345:Neisseria meningitidis / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods; D018272:Porins / Q000235:genetics*; D011544:Pseudogenes / Q000235:genetics*; D011786:Quality Control; D017422:Sequence Analysis, DNA
chemical_list: D018272:Porins; C057699:porin protein, Neisseria