title: Effect of plasma processing and organosilane modifications of polyethylene on Aeromonas hydrophila biofilm formation.
abstract: The aim of our research was to study how the modifications of polyethylene--a material commonly used in medicine and water industry--influence bacterial cell attachment and biofilm formation. The native surface was activated and modified using two-step process consisting in the activation of native surface with a H2O vapor plasma followed by its treatment with various organosilanes, namely, [3(tertbutylamine-2hydroxy) propyloxypropyl] diethoxymethylsilane, 1H,1H,2H,2H-perfluorooctylmethyldimethoxysilane, dimethoxydimethylsilane, and isobutylmethyldimethoxysilane. The effect of polyethylene modification after chemical treatment was analyzed using surface tension measurement. The adhesive properties of Aeromonas hydrophila LOCK0968 were studied in water with a low concentration of organic compounds, using luminometric and microscopic methods, and the viability of the adhered bacterial cells was evaluated using the colony forming units method. After two-week incubation the chemically modified materials exhibited better antiadhesive and antibacterial characteristics in comparison to the native surface. Among the examined modifying agents, dimethoxydimethylsilane showed the best desired properties.
authors.affiliation: Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Ulica Wolczanska 171/173, 90-924 Lodz, Poland.
authors.forename: Dorota
authors.identifier: 0000-0002-4006-6464
authors.initials: D
authors.lastname: Kregiel
authors.affiliation: Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Ulica Wolczanska 171/173, 90-924 Lodz, Poland.
authors.forename: Kamila
authors.identifier: 0000-0003-3598-5914
authors.initials: K
authors.lastname: Niedzielska
mesh_terms: D016980:Aeromonas hydrophila / Q000187:drug effects / Q000502:physiology*; D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D050296:Microbial Viability / Q000187:drug effects; D017646:Organosilicon Compounds / Q000494:pharmacology*; D058626:Plasma Gases / Q000494:pharmacology*; D020959:Polyethylene / Q000737:chemistry / Q000494:pharmacology*; D013500:Surface Tension / Q000187:drug effects
chemical_list: D017646:Organosilicon Compounds; D058626:Plasma Gases; D020959:Polyethylene