title: Metal enhanced fluorescence on nanoporous gold leaf-based assay platform for virus detection.
abstract: In the present study, a rapid, sensitive and quantitative detection of influenza A virus targeting hemagglutinin (HA) was developed using hybrid structure of quantum dots (QDs) and nanoporous gold leaf (NPGL). NPGL film was prepared by dealloying bimetallic film where its surface morphology and roughness were fairly controlled. Anti-influenza A virus HA antibody (ab66189) was bound with NPGL and amine (-NH2) terminated QDs. These biofunctionalized NPGL and QDs formed a complex with the influenza virus A/Beijing/262/95 (H1N1) and the photoluminescence (PL) intensities of QDs were linearly correlated with the concentrations of the virus up to 1ng/mL while no PL was observed in the absence of the virus, or in bovine serum albumin (BSA, 1µg/mL) alone. In addition, it was demonstrated that this assay detected successfully influenza virus A/Yokohama/110/2009 (H3N2) that is isolated from a clinical sample, at a concentration of ca. 50 plaque forming units (PFU)/mL. This detection limit is 2-order more sensitive than a commercially available rapid influenza diagnostic test. From these results, the proposed assay may offer a new strategy to monitor influenza virus for public health.
authors.affiliation: Graduate School of Science and Technology, Shizuoka University, 836 Ohya Suruga-ku, Shizuoka 422-8529, Japan; Department of Nano Fusion Technology, Pusan National University, Miryang 627-706, Republic of Korea. Electronic address: rahin_sust@yahoo.com.
authors.forename: Syed Rahin
authors.initials: SR
authors.lastname: Ahmed
authors.affiliation: Department of Nano Fusion Technology, Pusan National University, Miryang 627-706, Republic of Korea. Electronic address: ashraf3521@gmail.com.
authors.forename: Md Ashraf
authors.initials: MA
authors.lastname: Hossain
authors.affiliation: National Fisheries Research and Development Institute, Busan 619-705, Republic of Korea. Electronic address: jypark@nfrdi.go.kr.
authors.forename: Jung Youn
authors.initials: JY
authors.lastname: Park
authors.affiliation: Department of Nano Fusion Technology, Pusan National University, Miryang 627-706, Republic of Korea. Electronic address: sookim@pusan.ac.kr.
authors.forename: Soo-Hyung
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Nano Fusion Engineering and Cogno-Mechatronics Engineering, Pusan National University, Busan 609-735, Republic of Korea. Electronic address: dlee@pusan.ac.kr.
authors.forename: Dongyun
authors.initials: D
authors.lastname: Lee
authors.affiliation: Department of Infectious Diseases, Hamamatsu University School of Medicine, 1-20-1 Higashi-ku, Handa-yama, Hamamatsu 431-3192, Japan. Electronic address: tesuzuki@hama-med.ac.jp.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: Department of Nano Fusion Engineering and Cogno-Mechatronics Engineering, Pusan National University, Busan 609-735, Republic of Korea. Electronic address: jaebeom@pusan.ac.kr.
authors.forename: Jaebeom
authors.initials: J
authors.lastname: Lee
authors.affiliation: Graduate School of Science and Technology, Shizuoka University, 836 Ohya Suruga-ku, Shizuoka 422-8529, Japan; Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya Suruga-ku, Shizuoka 422-8529, Japan. Electronic address: acypark@ipc.shizuoka.ac.jp.
authors.forename: Enoch Y
authors.initials: EY
authors.lastname: Park
mesh_terms: D006046:Gold / Q000737:chemistry*; D053118:Influenza A Virus, H1N1 Subtype / Q000737:chemistry* / Q000302:isolation & purification* / Q000648:ultrastructure; D008163:Luminescent Measurements / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure*; D058608:Nanopores / Q000648:ultrastructure*; D010316:Particle Size; D013050:Spectrometry, Fluorescence / Q000379:methods*; D019562:Viral Load / Q000379:methods*
chemical_list: D006046:Gold
keywords: Fluorescence enhancement; Influenza A virus; Nanoporous gold leaf; Quantum dot; Surface roughness