title: Development of a monoclonal antibody-based immunochemical assay for liquiritin and its application to the quality control of licorice products.
abstract: Liquiritin was reacted with a keyhole limpet hemocyanin (KLH) to synthesize a liquiritin-KLH conjugate as an immunogen for mice. A hybridoma cell line named 2F8 secreted a monoclonal antibody (mAb) against liquiritin, which was applied to an enzyme-linked immunosorbent assay (ELISA) for liquiritin. ELISA showed a good linear range from 0.39 to 25 μg/mL of liquiritin. The maximum relative standard deviation (RSD) values for the intra-assay and interassay were approximately 5%. The recovery rates of liquiritin were in the range of 100.9-103.7%, and the concentrations of liquiritin in various licorice roots, as determined by ELISA, showed a good correlation with those analyzed by high-performance liquid chromatography (HPLC; R² = 0.948). These results suggested that ELISA with anti-liquiritin mAb could be a simple, rapid, convenient, and accurate method for the high-throughput analysis of liquiritin in various licorice products including liqueurs, sweets, and food supplements.
authors.affiliation: Department of Health and Nutrition, Faculty of Health Management, and ‡Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Nagasaki International University , 2825-7 Huis Ten Bosch, Sasebo 859-3298 Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Fujii
authors.forename: Osamu
authors.initials: O
authors.lastname: Morinaga
authors.forename: Takuhiro
authors.initials: T
authors.lastname: Uto
authors.forename: Shuichi
authors.initials: S
authors.lastname: Nomura
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Shoyama
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D044950:Flavanones / Q000032:analysis*; D005503:Food Additives / Q000032:analysis*; D005960:Glucosides / Q000032:analysis*; D006035:Glycyrrhiza / Q000737:chemistry*; D011786:Quality Control
chemical_list: D000911:Antibodies, Monoclonal; D044950:Flavanones; D005503:Food Additives; D005960:Glucosides; C512196:liquiritin