title: Biosynthesis of mupirocin by Pseudomonas fluorescens NCIMB 10586 involves parallel pathways.
abstract: Mupirocin, a clinically important antibiotic produced via a trans-AT Type I polyketide synthase (PKS) in Pseudomonas fluorescens, consists of a mixture of mainly pseudomonic acids A, B, and C. Detailed metabolic profiling of mutant strains produced by systematic inactivation of PKS and tailoring genes, along with re-feeding of isolated metabolites to mutant stains, has allowed the isolation of a large number of novel metabolites, identification of the 10,11-epoxidase, and full characterization of the mupirocin biosynthetic pathway, which proceeds via major (10,11-epoxide) and minor (10,11-alkene) parallel pathways.
authors.affiliation: School of Chemistry, University of Bristol , Bristol BS8 1TS, U.K.
authors.forename: Shu-Shan
authors.initials: SS
authors.lastname: Gao
authors.forename: Joanne
authors.initials: J
authors.lastname: Hothersall
authors.forename: Ji'en
authors.initials: J
authors.lastname: Wu
authors.forename: Annabel C
authors.initials: AC
authors.lastname: Murphy
authors.forename: Zhongshu
authors.initials: Z
authors.lastname: Song
authors.forename: Elton R
authors.initials: ER
authors.lastname: Stephens
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Thomas
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Crump
authors.forename: Russell J
authors.initials: RJ
authors.lastname: Cox
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Simpson
authors.forename: Christine L
authors.initials: CL
authors.lastname: Willis
mesh_terms: D008968:Molecular Conformation; D016712:Mupirocin / Q000096:biosynthesis* / Q000737:chemistry; D048630:Polyketide Synthases / Q000235:genetics / Q000378:metabolism*; D011551:Pseudomonas fluorescens / Q000201:enzymology* / Q000378:metabolism
chemical_list: D048630:Polyketide Synthases; D016712:Mupirocin