title: The de novo transcriptome and its analysis in the worldwide vegetable pest, Delia antiqua (Diptera: Anthomyiidae).
abstract: The onion maggot Delia antiqua is a major insect pest of cultivated vegetables, especially the onion, and a good model to investigate the molecular mechanisms of diapause. To better understand the biology and diapause mechanism of the insect pest species, D. antiqua, the transcriptome was sequenced using Illumina paired-end sequencing technology. Approximately 54 million reads were obtained, trimmed, and assembled into 29,659 unigenes, with an average length of 607 bp and an N50 of 818 bp. Among these unigenes, 21,605 (72.8%) were annotated in the public databases. All unigenes were then compared against Drosophila melanogaster and Anopheles gambiae. Codon usage bias was analyzed and 332 simple sequence repeats (SSRs) were detected in this organism. These data represent the most comprehensive transcriptomic resource currently available for D. antiqua and will facilitate the study of genetics, genomics, diapause, and further pest control of D. antiqua.
authors.affiliation: Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing, People's Republic of China.
authors.forename: Yu-Juan
authors.initials: YJ
authors.lastname: Zhang
authors.affiliation: Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing, People's Republic of China.
authors.forename: Youjin
authors.initials: Y
authors.lastname: Hao
authors.affiliation: Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing, People's Republic of China.
authors.forename: Fengling
authors.initials: F
authors.lastname: Si
authors.affiliation: Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing, People's Republic of China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Ren
authors.affiliation: Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing, People's Republic of China.
authors.forename: Ganyu
authors.initials: G
authors.lastname: Hu
authors.affiliation: Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Shen
authors.affiliation: Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing, People's Republic of China c_bin@hotmail.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
mesh_terms: D000818:Animals; D003062:Codon; D019295:Computational Biology; D004175:Diptera / Q000235:genetics*; D020224:Expressed Sequence Tags; D005260:Female; D020869:Gene Expression Profiling; D049750:Genome, Insect; D023281:Genomics; D059014:High-Throughput Nucleotide Sequencing; D008297:Male; D018895:Microsatellite Repeats; D058977:Molecular Sequence Annotation; D059467:Transcriptome; D014675:Vegetables / Q000469:parasitology*
chemical_list: D003062:Codon
keywords: codon usage bias; de novo assembly; high-throughput RNA sequencing; onion maggot; simple sequence repeat