title: Unilateral lesion increases oestrogen receptor α expression in the intact side of the ventromedial hypothalamic nucleus in ovariectomised rats.
abstract: To determine the relationship between the right and left sides of the ventrolateral ventromedial hypothalamic nucleus (vlVMN) in regulating the expression of oestrogen receptor (ER)α, the unilateral vlVMN was lesioned and the number of ERα-immunoreactive cells and the ERα mRNA level in the intact side of the vlVMN and arcuate nucleus (ARC) were measured in ovariectomised rats. Twenty-four hours after lesioning, brain samples were collected for analysis of ERα expression by immunohistochemistry and the real-time reverse transcriptase-polymerase chain reaction. The number of ERα-immunoreactive cells in the intact side of the vlVMN but not the ARC in the unilateral lesioned group was significantly higher than that in the control or sham-lesioned group. Expression levels of ERα mRNA in the intact side of the vlVMN but not the ARC in unilateral lesioned rats were significantly higher than those in the sham-lesioned group. Of transcript variants with alternative 5'-untranslated regions (0S, 0N, 0, 0T and E1), the ERα 0 transcript level was significantly increased. These results indicate that unilateral damage of vlVMN induces an increase in ERα in the intact side by increasing ERα transcription in a promoter-specific manner. The findings also suggest the existence of new neuroendocrine control system between the right and left sides for the expression of ERα in the vlVMN.
authors.affiliation: Neuroendocrinology, Department of Human Behaviour and Environment Sciences, Faculty of Human Sciences, Waseda University, Tokorozawa, Saitama, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Shimogawa
authors.forename: F
authors.initials: F
authors.lastname: Maekawa
authors.forename: K
authors.initials: K
authors.lastname: Yamanouchi
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D047628:Estrogen Receptor alpha / Q000378:metabolism*; D005260:Female; D010052:Ovariectomy; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D014697:Ventromedial Hypothalamic Nucleus / Q000378:metabolism* / Q000473:pathology
chemical_list: D017931:DNA Primers; D047628:Estrogen Receptor alpha
keywords: compensation; oestrogen receptor α; unilateral lesion; ventromedial hypothalamic nucleus