title: High-molecular-weight polyethylene glycol enhances hypothermic storage of feline kidney cells.
abstract: Phosphate-buffered sucrose (PBSc) solution is effective for short-term hypothermic preservation of tissue during feline kidney transplantation. A high-molecular-weight polyethylene glycol (35,000 Da, PEG35) reportedly enhanced the protective effects against cold-induced tubular injuries in animal kidney transplantation models. We investigated the ability of PBSc solution containing PEG35 to preserve cultured feline kidney cells using in vitro WST-8 cell proliferation assays. PEG35 significantly improved cell viability during 24 hr of cold preservation. PBSc containing 20 g/l PEG35 achieved an effect almost equal to that of University of Wisconsin (UW) solution, the gold standard preservation solution used in human clinical kidney transplantation, for up to 24 hr of preservation. Our results suggest that PBSc containing PEG35 provides an excellent medium for graft cold storage during feline kidney transplantation.
authors.affiliation: Division of Small Animal Surgery, Co-Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Katayama
authors.forename: Shinobu
authors.initials: S
authors.lastname: Tsuchiaka
authors.forename: Tomoki
authors.initials: T
authors.lastname: Motegi
authors.forename: Masao
authors.initials: M
authors.lastname: Miyazaki
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Yamashita
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Shimamura
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Okamura
authors.forename: Yuji
authors.initials: Y
authors.lastname: Uzuka
mesh_terms: D000818:Animals; D002415:Cats; D049109:Cell Proliferation; D002470:Cell Survival; D007668:Kidney; D016030:Kidney Transplantation / Q000379:methods*; D019188:Organ Preservation Solutions / Q000737:chemistry*; D010710:Phosphates / Q000032:analysis; D011092:Polyethylene Glycols / Q000737:chemistry*; D013395:Sucrose / Q000737:chemistry*; D013997:Time Factors
chemical_list: D019188:Organ Preservation Solutions; D010710:Phosphates; D011092:Polyethylene Glycols; D013395:Sucrose