title: Urease-based ISFET biosensor for arginine determination.
abstract: In this work a novel biosensor for arginine determination based on the urease inhibition effect has been proposed. Ion-selective field effect transistors were used as transducers. Urease immobilized in glutaraldehyde vapor served as a biorecognition element of the biosensor. Significant part of the work was aimed at proving the urease inhibition by arginine. Optimal concentration of urea for arginine determination was chosen. Detection limit for arginine was 0.05 mM. The biosensor selectivity towards different amino acids was studied. The results of quantitative determination of l-arginine in the real sample (a drinkable solution "Arginine Veyron") were in good agreement with the producer's data (a relative error was 5.2%). The biosensor showed a good reproducibility of arginine determination.
authors.affiliation: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Zabolotnogo Street 150, 03143 Kyiv, Ukraine; Max Planck Institute for Polymer Research, Ackermannweg 10, D-55128 Mainz, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Sheliakina
authors.affiliation: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Zabolotnogo Street 150, 03143 Kyiv, Ukraine.
authors.forename: V
authors.initials: V
authors.lastname: Arkhypova
authors.affiliation: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Zabolotnogo Street 150, 03143 Kyiv, Ukraine; Institute of High Technologies, Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, 01601 Kyiv, Ukraine.
authors.forename: O
authors.initials: O
authors.lastname: Soldatkin
authors.affiliation: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Zabolotnogo Street 150, 03143 Kyiv, Ukraine.
authors.forename: O
authors.initials: O
authors.lastname: Saiapina
authors.affiliation: Micro and Nanotechnology Department, Middle East Technical University, Dumlupınar Blv. 1, 06531 Ankara, Turkey; Central Laboratory, Middle East Technical University, Dumlupınar Blv. 1, 06531 Ankara, Turkey.
authors.forename: B
authors.initials: B
authors.lastname: Akata
authors.affiliation: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Zabolotnogo Street 150, 03143 Kyiv, Ukraine; Institute of High Technologies, Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, 01601 Kyiv, Ukraine. Electronic address: dzyad@yahoo.com.
authors.forename: S
authors.initials: S
authors.lastname: Dzyadevych
mesh_terms: D000818:Animals; D001120:Arginine / Q000032:analysis*; D015374:Biosensing Techniques; D002417:Cattle; D057230:Limit of Detection; D014510:Urease / Q000737:chemistry*
chemical_list: D001120:Arginine; D014510:Urease
keywords: Arginine; Biosensor; Inhibition; Ion-selective field effect transistors; Urease