title: Interaction of hyaluronan binding peptides with glycosaminoglycans in poly(ethylene glycol) hydrogels.
abstract: This study investigates the incorporation of hyaluronan (HA) binding peptides into poly(ethylene glycol) (PEG) hydrogels as a mechanism to bind and retain hyaluronan for applications in tissue engineering. The specificity of the peptide sequence (native RYPISRPRKRC vs non-native RPSRPRIRYKC), the role of basic amino acids, and specificity to hyaluronan over other GAGs in contributing to the peptide-hyaluronan interaction were probed through experiments and simulations. Hydrogels containing the native or non-native peptide retained hyaluronan in a dose-dependent manner. Ionic interactions were the dominating mechanism. In diH2O the peptides interacted strongly with HA and chondroitin sulfate, but in phosphate buffered saline the peptides interacted more strongly with HA. For cartilage tissue engineering, chondrocyte-laden PEG hydrogels containing increasing amounts of HA binding peptide and exogenous HA had increased retention and decreased loss of cell-secreted proteoglycans in and from the hydrogel at 28 days. This new matrix-interactive hydrogel platform holds promise for tissue regeneration.
authors.affiliation: Department of Chemical and Biological Engineering, ‡BioFrontiers Institute, and §Materials Science and Engineering Program, University of Colorado , Boulder, Colorado 80309.
authors.forename: Justine J
authors.initials: JJ
authors.lastname: Roberts
authors.forename: Robert M
authors.initials: RM
authors.lastname: Elder
authors.forename: Alexander J
authors.initials: AJ
authors.lastname: Neumann
authors.forename: Arthi
authors.initials: A
authors.lastname: Jayaraman
authors.forename: Stephanie J
authors.initials: SJ
authors.lastname: Bryant
mesh_terms: D000818:Animals; D001665:Binding Sites; D001672:Biocompatible Materials; D002356:Cartilage; D002417:Cattle; D019902:Chondrocytes / Q000166:cytology*; D006025:Glycosaminoglycans / Q000737:chemistry / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000378:metabolism*; D020100:Hydrogels / Q000737:chemistry*; D056004:Molecular Dynamics Simulation; D009994:Osmolar Concentration; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D006025:Glycosaminoglycans; D020100:Hydrogels; D010455:Peptides; D011092:Polyethylene Glycols; D006820:Hyaluronic Acid