title: Annual FEV1 changes and numbers of circulating endothelial microparticles in patients with COPD: a prospective study.
abstract: OBJECTIVE
  Growing evidence suggests that endothelial injury is involved in the pathophysiology of chronic obstructive pulmonary disease (COPD). Circulating endothelial microparticles (EMPs) increase in patients with COPD because of the presence of endothelial injury. We examined the relationship between EMP number and changes in forced expiratory volume in 1 s (FEV1) in patients with COPD.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  One hospital in Japan.
  
  
  METHODS
  A total 48 outpatients with stable COPD coming to the hospital from September 2010 to September 2011.
  Blood samples were collected and vascular endothelial (VE)-cadherin EMPs (CD144+ EMPs), E-selectin EMPs (CD62E+ EMPs) and platelet endothelial cell adhesion molecule EMPs (CD31+/CD41- EMPs) were measured using fluorescence-activated cell sorting. Annual FEV1 changes were evaluated using FEV1 data acquired a year before and a year after sample collection.
  
  
  RESULTS
  The number of E-selectin and VE-cadherin EMPs showed significant negative correlations with annual FEV1 changes (rs=-0.65, p<0.001, rs=-0.43, p=0.003, respectively). Leucocyte counts tended to be correlated with annual FEV1 changes, but this correlation was not significant (rs=-0.28, p=0.057). There were significant differences in annual FEV1 changes between with and without history of frequent exacerbation (p=0.006), and among Global Initiative for Chronic Obstructive Lung Disease (GOLD) stages (p=0.009). Multiple linear regression analysis revealed E-selectin EMP to be the only significant parameter associated with annual FEV1 changes, independent of VE-cadherin EMP, GOLD stages, leucocyte counts, and history of frequent exacerbation. Receiver operating characteristic curves showed the optimum E-selectin EMP cut-off level for prediction of rapid FEV1 decline (>66 mL/year) to be 153.0/µL (areas under curve 0.78 (95% CI 0.60 to 0.89); sensitivity, 67%; specificity, 81%).
  
  
  CONCLUSIONS
  The high E-selectin EMP levels in stable patients with COPD are predictive of rapid FEV1 decline.
  
  
  BACKGROUND
  UMIN000005168.
authors.affiliation: Department of Advanced Preventive Medicine for Infectious Disease, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Takahashi
authors.forename: Seiichi
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Naoya
authors.initials: N
authors.lastname: Fujino
authors.forename: Takaya
authors.initials: T
authors.lastname: Suzuki
authors.forename: Chiharu
authors.initials: C
authors.lastname: Ota
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Tando
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Yamada
authors.forename: Masaru
authors.initials: M
authors.lastname: Yanai
authors.forename: Mutsuo
authors.initials: M
authors.lastname: Yamaya
authors.forename: Shin
authors.initials: S
authors.lastname: Kurosawa
authors.forename: Masanori
authors.initials: M
authors.lastname: Yamauchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kubo
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D015820:Cadherins / Q000097:blood; D002196:Capillaries / Q000503:physiopathology; D055252:Cell-Derived Microparticles / Q000378:metabolism*; D018450:Disease Progression; D019040:E-Selectin / Q000097:blood; D004730:Endothelium, Vascular / Q000378:metabolism*; D005541:Forced Expiratory Volume; D006801:Humans; D007958:Leukocyte Count; D008168:Lung / Q000098:blood supply; D011446:Prospective Studies; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood / Q000503:physiopathology*; D012737:Sex Factors
chemical_list: D015415:Biomarkers; D015820:Cadherins; D019040:E-Selectin