title: Association of BH3 interacting domain death agonist (BID) gene polymorphisms with proteinuria of immunoglobulin A nephropathy.
abstract: BACKGROUND
  Apoptosis plays an important role in the mechanism regulating the development of glomerulonephritis. We investigated whether polymorphisms of apoptotic genes such as B-cell CLL/lymphoma 2 (BCL2), BH3-interacting domain death agonist (BID), and caspase 8 (CASP8) were associated with immunoglobulin A nephropathy (IgAN) and with the clinical phenotypes of IgAN patients.
  
  
  METHODS
  We genotyped promoter and coding region single nucleotide polymorphisms (SNPs) (rs2279115 and rs1801018 for BCL2; rs8190315 and rs2072392 for BID; and rs6747918 and rs1045487 for CASP8) using direct sequencing in 195 IgAN patients and 289 control subjects.
  
  
  RESULTS
  No SNPs were associated with IgAN. However, in analysis of clinical phenotypes, we found that rs8190315 and rs2072392 of BID were associated with proteinuria levels of IgAN patients in additive (AG vs. GG vs. AA, p = 0.0008 for rs8190315; TC vs. CC vs. TT, p = 0.0012 for rs2072392) and dominant models (AG/GG vs. AA, p = 0.0014 for rs8190315; TC/CC vs. TT, p = 0.0031 for rs2072392). In particular, the frequencies of genotypes containing minor alleles of rs8190315 (G allele) and rs2072392 (C allele) were increased in IgAN patients with higher protienuria levels (> 40 mg/m(2)/h).
  
  
  CONCLUSIONS
  These results suggest that BID may play a role in severe IgAN.
authors.affiliation: Kohwang Medical Research Institute, School of Medicine, Kyung Hee University , Seoul , Republic of Korea.
authors.forename: Hae Jeong
authors.initials: HJ
authors.lastname: Park
authors.forename: Jong Woo
authors.initials: JW
authors.lastname: Kim
authors.forename: Byoung-Soo
authors.initials: BS
authors.lastname: Cho
authors.forename: Joo-Ho
authors.initials: JH
authors.lastname: Chung
mesh_terms: D000293:Adolescent; D051023:BH3 Interacting Domain Death Agonist Protein / Q000235:genetics*; D016022:Case-Control Studies; D053181:Caspase 8 / Q000235:genetics; D002648:Child; D005260:Female; D020022:Genetic Predisposition to Disease; D005922:Glomerulonephritis, IGA / Q000150:complications / Q000235:genetics*; D006801:Humans; D016015:Logistic Models; D008297:Male; D020641:Polymorphism, Single Nucleotide; D011507:Proteinuria / Q000209:etiology / Q000235:genetics*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics
chemical_list: D051023:BH3 Interacting Domain Death Agonist Protein; C494814:BID protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; C505530:CASP8 protein, human; D053181:Caspase 8
keywords: BH3-interacting domain death agonist; immunoglobulin A nephropathy; proteinuria; single nucleotide polymorphism