title: Detection of avian paramyxoviruses in migratory and resident birds in the state of Rio de Janeiro, Brazil.
abstract: Paramyxoviruses and avian influenza viruses are present worldwide, and wild birds are known natural reservoirs of these viruses. This study monitored the circulation of these viruses in migratory and resident coastal birds captured in the state of Rio de Janeiro, Brazil. In total, 494 birds were trapped, and their fecal samples were collected and inoculated into embryonated chicken eggs. The allantoic fluids were evaluated using a hemagglutination test and PCR amplification of the genes of the M and L proteins of influenza A virus and paramyxovirus, respectively. Avian paramyxovirus was detected in 5 (1.01%) of the birds. The majority of these viruses were isolated from migratory birds classified into the order Charadriiformes (families Scolopacidae and Charadriidae). Four samples were characterized as avian paramyxovirus serotype-2 (APMV-2) by a hemagglutination inhibition test. These results reinforce the importance of continuous surveillance of wild species in Brazil.
authors.affiliation: Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Cidade Universitaria, Avenida Carlos Chagas Filho, 373, 21941-902, Rio de Janeiro, RJ, Brazil.
authors.forename: Luz Alba M G
authors.initials: LA
authors.lastname: Fornells
authors.affiliation: Laboratório de Sanidade Animal, Universidade Estadual do Norte Fluminense, Avenida Alberto Lamego, 2000, Horto, 28015-620, Campos dos Goytacazes, RJ, Brazil.
authors.forename: Carlos E P F
authors.initials: CE
authors.lastname: Travassos
authors.affiliation: Laboratório de Sanidade Animal, Universidade Estadual do Norte Fluminense, Avenida Alberto Lamego, 2000, Horto, 28015-620, Campos dos Goytacazes, RJ, Brazil.
authors.forename: Claudia M
authors.initials: CM
authors.lastname: Costa
authors.affiliation: Laboratório de Sanidade Animal, Universidade Estadual do Norte Fluminense, Avenida Alberto Lamego, 2000, Horto, 28015-620, Campos dos Goytacazes, RJ, Brazil.
authors.forename: Ronaldo
authors.initials: R
authors.lastname: Novelli
authors.affiliation: Laboratório de Sanidade Animal, Universidade Estadual do Norte Fluminense, Avenida Alberto Lamego, 2000, Horto, 28015-620, Campos dos Goytacazes, RJ, Brazil.
authors.forename: Melissa P
authors.initials: MP
authors.lastname: Petrucci
authors.affiliation: Laboratório de Sanidade Animal, Universidade Estadual do Norte Fluminense, Avenida Alberto Lamego, 2000, Horto, 28015-620, Campos dos Goytacazes, RJ, Brazil.
authors.forename: Flavio L
authors.initials: FL
authors.lastname: Soffiati
authors.affiliation: Laboratório de Sanidade Animal, Universidade Estadual do Norte Fluminense, Avenida Alberto Lamego, 2000, Horto, 28015-620, Campos dos Goytacazes, RJ, Brazil.
authors.forename: Iliani
authors.initials: I
authors.lastname: Bianchi
authors.affiliation: Laboratório de Sanidade Animal, Universidade Estadual do Norte Fluminense, Avenida Alberto Lamego, 2000, Horto, 28015-620, Campos dos Goytacazes, RJ, Brazil.
authors.forename: Luiz F Lino
authors.initials: LF
authors.lastname: de Souza
authors.affiliation: Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Cidade Universitaria, Avenida Carlos Chagas Filho, 373, 21941-902, Rio de Janeiro, RJ, Brazil.
authors.forename: Venício F
authors.initials: VF
authors.lastname: Veiga
authors.affiliation: Laboratório de Biologia Animal-Setor de Biotecnologia, Empresa de Pesquisa do Estado do Rio de Janeiro, Alameda São Boaventura, 770, Pesagro, Fonseca, 24120-191, Niterói, RJ, Brazil.
authors.forename: Maíra H T
authors.initials: MH
authors.lastname: Liberal
authors.affiliation: Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Cidade Universitaria, Avenida Carlos Chagas Filho, 373, 21941-902, Rio de Janeiro, RJ, Brazil.
authors.forename: José Nelson S S
authors.initials: JN
authors.lastname: Couceiro
mesh_terms: D025041:Animal Migration; D000818:Animals; D018109:Avulavirus / Q000302:isolation & purification*; D045463:Avulavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D001715:Bird Diseases / Q000453:epidemiology* / Q000821:virology; D001717:Birds; D001938:Brazil / Q000453:epidemiology; D005243:Feces / Q000821:virology; D006386:Hemagglutination Tests / Q000662:veterinary; D009980:Influenza A virus / Q000302:isolation & purification; D005585:Influenza in Birds / Q000453:epidemiology / Q000821:virology; D008854:Microscopy, Electron / Q000662:veterinary; D010063:Ovum / Q000821:virology; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012621:Seasons
chemical_list: D012367:RNA, Viral