title: Rapid determination of the amino acid configuration of xenotetrapeptide.
abstract: An E. coli strain with deletions in five transaminases (ΔaspC ΔilvE ΔtyrB ΔavtA ΔybfQ) was constructed to be unable to degrade several amino acids. This strain was used as an expression host for the analysis of the amino acid configuration of nonribosomally synthesized peptides, including the novel peptide "xenotetrapeptide" from Xenorhabdus nematophila, by using a combination of labeling experiments and mass spectrometry. Additionally, the number of D-amino acids in the produced peptide was assigned following simple cultivation of the expression strain in D2 O.
authors.affiliation: Merck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Goethe Universität Frankfurt, Max-von-Laue-Strasse 9, 60438 Frankfurt am Main (Germany).
authors.forename: Carsten
authors.initials: C
authors.lastname: Kegler
authors.forename: Friederike I
authors.initials: FI
authors.lastname: Nollmann
authors.forename: Tilman
authors.initials: T
authors.lastname: Ahrendt
authors.forename: Florian
authors.initials: F
authors.lastname: Fleischhacker
authors.forename: Edna
authors.initials: E
authors.lastname: Bode
authors.forename: Helge B
authors.initials: HB
authors.lastname: Bode
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003903:Deuterium / Q000737:chemistry; D004926:Escherichia coli / Q000201:enzymology; D029968:Escherichia coli Proteins / Q000378:metabolism; D013058:Mass Spectrometry; D008968:Molecular Conformation; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D000637:Transaminases / Q000378:metabolism; D020593:Xenorhabdus / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D010455:Peptides; D003903:Deuterium; D000637:Transaminases
keywords: D-amino acids; absolute configuration; deuterium exchange; mass spectrometry; peptides; structure elucidation