title: Preparation of przewalskinic acid A from salvianolic acid B using a crude enzyme from an Aspergillus oryzae strain.
abstract: Przewalskinic acid A is a rare, water-soluble, and highly biologically active ingredient found, thus far, only in the Salvia przewalskii Maxim herb; however, the content in S. przewalskii herb is very low. In order to obtain useful quantities of przewalskinic acid A, the biotransformatin of salvianolic acid B from Salvia miltiorrhiza root (danshen in Chinese) into przewalskinic acid A was studied using a crude enzyme produced from Aspergillus oryzae D30s strain. The crude enzyme from the A. oryzae strain hydrolyzed salvianolic acid B into przewalskinic acid A and danshensu. The preparation afforded 31.3 g przewalskinic acid A (91.0 % purity) and 13.1 g danshensu (95 % purity) from 75 g salvianolic acid B. The preparation of przewalskinic acid A was therefore very successful with a yield of over 86 %, but the yield of danshensu was only 33 %. The product przewalskinic acid A was identified using ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) and NMR.
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Science, Jilin University, Changchun, 130012, People's Republic of China.
authors.forename: Chunying
authors.initials: C
authors.lastname: Liu
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Jin
authors.forename: Hongshan
authors.initials: H
authors.lastname: Yu
authors.forename: Changkai
authors.initials: C
authors.lastname: Sun
authors.forename: Shuiping
authors.initials: S
authors.lastname: Zhou
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Zhu
authors.forename: Longquan
authors.initials: L
authors.lastname: Xu
authors.forename: Chong
authors.initials: C
authors.lastname: Yu
authors.forename: Fengxie
authors.initials: F
authors.lastname: Jin
mesh_terms: D001236:Aspergillus oryzae / Q000201:enzymology*; D001572:Benzofurans / Q000737:chemistry / Q000378:metabolism*; D001711:Biotransformation; D007773:Lactates / Q000737:chemistry / Q000378:metabolism; D053630:Depsides
chemical_list: D001572:Benzofurans; D007773:Lactates; C000591548:przewalskinic acid A; C035055:3,4-dihydroxyphenyllactic acid; C076944:salvianolic acid B; D053630:Depsides