title: C1A cysteine protease-cystatin interactions in leaf senescence.
abstract: Senescence-associated proteolysis in plants is a crucial process to relocalize nutrients from leaves to growing or storage tissues. The massive net degradation of proteins involves broad metabolic networks, different subcellular compartments, and several types of proteases and regulators. C1A cysteine proteases, grouped as cathepsin L-, B-, H-, and F-like according to their gene structures and phylogenetic relationships, are the most abundant enzymes responsible for the proteolytic activity during leaf senescence. Besides, cystatins as specific modulators of C1A peptidase activities exert a complex regulatory role in this physiological process. This overview article covers the most recent information on C1A proteases in leaf senescence in different plant species. Particularly, it is focussed on barley, as the unique species where the whole gene family members of C1A cysteine proteases and cystatins have been analysed.
authors.affiliation: Centro de Biotecnología y Genómica de Plantas UPM-INIA, Campus de Montegancedo, Universidad Politécnica de Madrid, Autovia M40 (Km 38), 28223-Pozuelo de Alarcon, Madrid, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Díaz-Mendoza
authors.affiliation: Centro de Biotecnología y Genómica de Plantas UPM-INIA, Campus de Montegancedo, Universidad Politécnica de Madrid, Autovia M40 (Km 38), 28223-Pozuelo de Alarcon, Madrid, Spain.
authors.forename: Blanca
authors.initials: B
authors.lastname: Velasco-Arroyo
authors.affiliation: Centro de Biotecnología y Genómica de Plantas UPM-INIA, Campus de Montegancedo, Universidad Politécnica de Madrid, Autovia M40 (Km 38), 28223-Pozuelo de Alarcon, Madrid, Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: González-Melendi
authors.affiliation: Centro de Biotecnología y Genómica de Plantas UPM-INIA, Campus de Montegancedo, Universidad Politécnica de Madrid, Autovia M40 (Km 38), 28223-Pozuelo de Alarcon, Madrid, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Martínez
authors.affiliation: Centro de Biotecnología y Genómica de Plantas UPM-INIA, Campus de Montegancedo, Universidad Politécnica de Madrid, Autovia M40 (Km 38), 28223-Pozuelo de Alarcon, Madrid, Spain i.diaz@upm.es.
authors.forename: Isabel
authors.initials: I
authors.lastname: Díaz
mesh_terms: D015891:Cystatins / Q000378:metabolism*; D057056:Cysteine Proteases / Q000378:metabolism*; D001467:Hordeum / Q000378:metabolism; D018515:Plant Leaves / Q000254:growth & development* / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D015891:Cystatins; D010940:Plant Proteins; D057056:Cysteine Proteases
keywords: Cystatin; cysteine protease; leaf senescence; nutrient recycling; protein breakdown; proteolysis.