title: Long non-coding RNA HOTAIR is targeted and regulated by miR-141 in human cancer cells.
abstract: HOTAIR is a long non-coding RNA that interacts with the polycomb repressive complex and suppresses its target genes. HOTAIR has also been demonstrated to promote malignancy. MicroRNA-141 (miR-141) has been reported to play a role in the epithelial to mesenchymal transition process, and the expression of miR-141 is inversely correlated with tumorigenicity and invasiveness in several human cancers. We found that HOTAIR expression is inversely correlated to miR-141 expression in renal carcinoma cells. HOTAIR promotes malignancy, including proliferation and invasion, whereas miR-141 suppresses malignancy in human cancer cells. miR-141 binds to HOTAIR in a sequence-specific manner and suppresses HOTAIR expression and functions, including proliferation and invasion. Both HOTAIR and miR-141 were associated with the immunoprecipitated Ago2 (Argonaute2) complex, and the Ago2 complex cleaved HOTAIR in the presence of miR-141. These results demonstrate that HOTAIR is suppressed by miR-141 in an Ago2-dependent manner.
authors.affiliation: From the Department of Urology, San Francisco Veterans Affairs Medical Center and University of California, San Francisco, San Francisco, California 94121.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Chiyomaru
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Fukuhara
authors.forename: Sharanjot
authors.initials: S
authors.lastname: Saini
authors.forename: Shahana
authors.initials: S
authors.lastname: Majid
authors.forename: Guoren
authors.initials: G
authors.lastname: Deng
authors.forename: Varahram
authors.initials: V
authors.lastname: Shahryari
authors.forename: Inik
authors.initials: I
authors.lastname: Chang
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Hideki
authors.initials: H
authors.lastname: Enokida
authors.forename: Masayuki
authors.initials: M
authors.lastname: Nakagawa
authors.forename: Rajvir
authors.initials: R
authors.lastname: Dahiya
authors.forename: Soichiro
authors.initials: S
authors.lastname: Yamamura
mesh_terms: D017209:Apoptosis / Q000235:genetics; D060565:Argonaute Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D015153:Blotting, Western; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D019073:HT29 Cells; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D009361:Neoplasm Invasiveness; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D034622:RNA Interference; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D000071799:Zinc Finger E-box-Binding Homeobox 1
chemical_list: C466422:AGO2 protein, human; D060565:Argonaute Proteins; C561566:HOTAIR long untranslated RNA, human; D018398:Homeodomain Proteins; C533895:MIRN141 microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding; D014157:Transcription Factors; C486307:ZEB1 protein, human; D000071799:Zinc Finger E-box-Binding Homeobox 1
keywords: Cancer Biology; Cell Growth; Gene Regulation; Genistein; HOTAIR; Invasion; Long Non-coding RNA; MicroRNA; Renal Carcinoma; miR-141