title: When half is better than the whole: advances in haploid embryonic stem cell technology.
abstract: Recent advances in embryonic stem cell (ESC) derivation and genome editing offer efficient platforms for genetic screening. In this issue, Li et al. and Leeb et al., respectively, expand such applications by generating haploid rat ESCs for screening, mutagenesis, and CRISPR-Cas-mediated gene targeting and by developing a forward genetic screen for interrogating haploid mESCs.
authors.affiliation: Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Chikara
authors.initials: C
authors.lastname: Kokubu
authors.affiliation: Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan. Electronic address: takeda@mr-envi.med.osaka-u.ac.jp.
authors.forename: Junji
authors.initials: J
authors.lastname: Takeda
mesh_terms: D000818:Animals; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D005821:Genetic Techniques; D005820:Genetic Testing; D006238:Haploidy; D006801:Humans; D008297:Male