title: First report of Pseudobodo sp, a new pathogen for a potential energy-producing algae: Chlorella vulgaris cultures.
abstract: Chlorella vulgaris, is a kind of single-celled green algae, which could serve as a potential source of food and energy because of its photosynthetic efficiency. In our study, a pathogenic organism targeting C. vulgaris was discovered. The algae-lytic activity relates to a fraction from lysates of infected C. vulgaris that was blocked upon filtration through a 3 µm filter. 18S rRNA gene sequence analysis revealed that it shared 99.0% homology with the protist Pseudobodo tremulans. Scanning electron microscope analysis showed that Pseudobodo sp. KD51 cells were approximately 4-5 µm long, biflagellate with an anterior collar around the anterior part of the cell in unstressed feeding cells. Besides the initial host, Pseudobodo sp. KD51 could also kill other algae, indicating its relatively wide predatory spectrum. Heat stability, pH and salinity tolerance experiments were conducted to understand their effects on its predatory activities, and the results showed that Pseudobodo sp. KD51 was heat-sensitive, and pH and salinity tolerant.
authors.affiliation: State Key Laboratory for Marine Environmental Sciences and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen, China; ShenZhen Research Institute of Xiamen University, ShenZhen, China.
authors.forename: Zhangran
authors.initials: Z
authors.lastname: Chen
authors.affiliation: State Key Laboratory for Marine Environmental Sciences and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen, China.
authors.forename: Xueqian
authors.initials: X
authors.lastname: Lei
authors.affiliation: State Key Laboratory for Marine Environmental Sciences and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen, China.
authors.forename: Bangzhou
authors.initials: B
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Marine Environmental Sciences and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen, China.
authors.forename: Luxi
authors.initials: L
authors.lastname: Yang
authors.affiliation: State Key Laboratory for Marine Environmental Sciences and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen, China.
authors.forename: Huajun
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Marine Environmental Sciences and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen, China.
authors.forename: Jingyan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Marine Environmental Sciences and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory for Marine Environmental Sciences and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
authors.affiliation: State Key Laboratory for Marine Environmental Sciences and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Bioengineering College of Jimei University, Xiamen, China.
authors.forename: Jingwen
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Marine Environmental Sciences and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen, China; ShenZhen Research Institute of Xiamen University, ShenZhen, China.
authors.forename: Tianling
authors.initials: T
authors.lastname: Zheng
mesh_terms: D056804:Biofuels; D048408:Chlorella vulgaris / Q000382:microbiology*; D054884:Host-Pathogen Interactions; D006863:Hydrogen-Ion Concentration; D058889:Molecular Typing; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D055049:Salt Tolerance; D058009:Stramenopiles / Q000235:genetics* / Q000648:ultrastructure
chemical_list: D056804:Biofuels; D012337:RNA, Ribosomal, 18S