title: Differences in expression of uroplakin III, cytokeratin 7, and cyclooxygenase-2 in canine proliferative urothelial lesions of the urinary bladder.
abstract: The expression of immunohistochemical markers that have been used in diagnosis and/or prognostication of urothelial tumors in humans (uroplakin III [UPIII], cytokeratin 7 [CK7], cyclooxygenase-2 [COX-2], and activated caspase 3) was evaluated in a series of 99 canine proliferative urothelial lesions of the urinary bladder and compared to the lesion classification and grade as defined by the World Health Organization / International Society of Urologic Pathology consensus system. There were significant associations between tumor classification and overall UPIII pattern (P = 1.49 × 10(-18)), loss of UPIII (P = 1.27 × 10(-4)), overall CK7 pattern (P = 4.34 × 10(-18)), and COX-2 pattern (P = 8.12 × 10(-25)). In addition, there were significant associations between depth of neoplastic cell infiltration into the urinary bladder wall and overall UPIII pattern (P = 1.54 × 10(-14)), loss of UPIII (P = 2.07 × 10(-4)), overall CK7 pattern (P = 1.17 × 10(-13)), loss of CK7 expression (P = .0485), and COX-2 pattern (P = 8.23 × 10(-21)). There were no significant associations between tumor classification or infiltration and caspase 3 expression pattern.
authors.affiliation: Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, MI, USA sledged@dcpah.msu.edu.
authors.forename: D G
authors.initials: DG
authors.lastname: Sledge
authors.affiliation: MPI Research, Mattawan, MI, USA.
authors.forename: D J
authors.initials: DJ
authors.lastname: Patrick
authors.affiliation: Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, MI, USA.
authors.forename: S D
authors.initials: SD
authors.lastname: Fitzgerald
authors.affiliation: Pharmanet/i3, Haslett, MI, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, MI, USA.
authors.forename: M
authors.initials: M
authors.lastname: Kiupel
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002295:Carcinoma, Transitional Cell / Q000378:metabolism* / Q000473:pathology; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D004285:Dogs; D007150:Immunohistochemistry / Q000662:veterinary; D053552:Keratin-7 / Q000378:metabolism*; D016612:Paraffin Embedding; D001743:Urinary Bladder / Q000378:metabolism / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000473:pathology; D060171:Uroplakin III / Q000378:metabolism*; D019459:Urothelium / Q000378:metabolism / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D053552:Keratin-7; D060171:Uroplakin III; D051546:Cyclooxygenase 2
keywords: cytokeratin; dog; immunohistochemistry; neoplasia; oncology; transitional cell carcinoma; urinary bladder; uroplakin III