title: Nutritional requirements and media development for Lactococcus lactis IL1403.
abstract: Lactic acid bacteria are extensively used in food technology and for the production of various compounds, but they are fastidious in nutrient requirements. In order to elucidate the role of each component precisely, defined multicomponent media are required. This study focuses on determining nutrient auxotrophies and minimizing media components (amino acids, vitamins, metal ions, buffers and additional compounds) for the cultivation of Lactococcus lactis subsp. lactis IL1403, using microtitre plates and test tubes. It was shown that glutamine and asparagine were the most important media components for achieving higher biomass yields while the branched-chain amino acids were necessary to increase specific growth rate. The amino acid and glucose ratio was reduced to achieve minimal residual concentration of amino acids in the medium after the growth of cells, whereas the specific growth rate and biomass yield of cells were not considerably affected. As the percentage of each consumed amino acid compared to initial amount is larger than measurement error, these optimized media are important for achieving more precise data about amino acid utilization and metabolism.
authors.affiliation: The Competence Center of Food and Fermentation Technologies (CCFFT), Akadeemia tee 15A, 12618, Tallinn, Estonia, aller@tftak.eu.
authors.forename: Kadri
authors.initials: K
authors.lastname: Aller
authors.forename: Kaarel
authors.initials: K
authors.lastname: Adamberg
authors.forename: Veronica
authors.initials: V
authors.lastname: Timarova
authors.forename: Andrus
authors.initials: A
authors.lastname: Seiman
authors.forename: Darja
authors.initials: D
authors.lastname: Feštšenko
authors.forename: Raivo
authors.initials: R
authors.lastname: Vilu
mesh_terms: D003470:Culture Media / Q000737:chemistry*; D013294:Lactococcus lactis / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D003470:Culture Media