title: Simultaneous determination of ethyl carbamate and urea in alcoholic beverages by high-performance liquid chromatography coupled with fluorescence detection.
abstract: On the basis of the similar fluorescence of ethyl carbamate (EC) and urea derivatives, a high-performance liquid chromatography method coupled with fluorescence detection was developed for the simultaneous determination of EC and urea in alcoholic beverages. The chromatographic separation and derivatization conditions of EC and urea were optimized. Under the established conditions, the detection limit, relative standard deviation, linear range, and recovery were 4.8 μg/L, 1.0-4.2%, 10-500 μg/L, and 93.8-104.6%, respectively, for EC; the corresponding values were 0.003 mg/L, 1.2-4.8%, 0.01-100 mg/L, and 90.7-104.8%, respectively, for urea. The method showed satisfactory values for precision, recovery, and sensitivity for both analytes and is well-suited for routine analysis and kinetic studies of the formation of EC from urea alcoholysis in alcoholic beverages.
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology ( Tianjin University of Science and Technology ), Ministry of Education, Tianjin 300457, P. R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Guoxin
authors.initials: G
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Gao
authors.forename: Depei
authors.initials: D
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
mesh_terms: D000434:Alcoholic Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D014508:Urea / Q000032:analysis*; D014520:Urethane / Q000032:analysis*
chemical_list: D014520:Urethane; D014508:Urea