title: Evaluation of the antimutagenic activity and mode of action of the fructooligosaccharide inulin in the meristematic cells of Allium cepa culture.
abstract: This study evaluated the mutagenicity and antimutagenicity of inulin in a chromosomal aberration assay in cultures of the meristematic cells of Allium cepa. The treatments evaluated were as follows: negative control--seed germination in distilled water; positive control--aqueous solution of methyl methanesulfonate (10 μg/mL MMS); mutagenicity--aqueous solutions of inulin (0.015, 0.15, and 1.50 μg/mL); and antimutagenicity--associations between MMS and the different inulin concentrations. The antimutagenicity protocols established were pre-treatment, simultaneous simple, simultaneous with pre-incubation, and post-treatment. The damage reduction percentage (DR%) was 43.56, 27.77, and 55.92% for the pre-treatment; -31.11, 18.51, and 7.03% for the simultaneous simple; 30.43, 19.12, and 21.11% for the simultaneous with pre-incubation; and 64.07, 42.96, and 53.70% for the post-treatment. The results indicated that the most effective treatment for inhibiting damages caused by MMS was the post-treatment, which was followed by the pre-treatment, suggesting activity by bioantimutagenesis and desmutagenesis. The Allium cepa assay was demonstrated to be a good screening test for this type of activity because it is easy to perform, has a low cost, and shows DR% that is comparable to that reported studies that evaluated the prevention of DNA damage in mammals by inulin.
authors.affiliation: Centro de Estudos em Células Tronco, Terapia Celular e Genética Toxicológica, Núcleo de Hospital Universitário, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil.
authors.forename: M O
authors.initials: MO
authors.lastname: Mauro
authors.affiliation: Centro de Estudos em Células Tronco, Terapia Celular e Genética Toxicológica, Núcleo de Hospital Universitário, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil.
authors.forename: J R
authors.initials: JR
authors.lastname: Pesarini
authors.affiliation: Programa de Pós-Graduação em Biologia Celular e Molecular, Instituto de Biociências de Rio Claro, Universidade Estadual Paulista, Rio Claro, SP, Brasil.
authors.forename: M A
authors.initials: MA
authors.lastname: Marin-Morales
authors.affiliation: Programa de Mestrado em Farmácia, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil.
authors.forename: M T F D
authors.initials: MT
authors.lastname: Monreal
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil.
authors.forename: A C D
authors.initials: AC
authors.lastname: Monreal
authors.affiliation: Departamento de Biologia, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, PR, Brasil.
authors.forename: M S
authors.initials: MS
authors.lastname: Mantovani
authors.affiliation: Centro de Estudos em Células Tronco, Terapia Celular e Genética Toxicológica, Núcleo de Hospital Universitário, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil rodrigo.oliveira@ufms.br.
authors.forename: R J
authors.initials: RJ
authors.lastname: Oliveira
mesh_terms: D016587:Antimutagenic Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000187:drug effects*; D004249:DNA Damage; D007444:Inulin / Q000494:pharmacology*; D018519:Meristem / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008741:Methyl Methanesulfonate / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008940:Mitotic Index; D009153:Mutagens / Q000494:pharmacology*; D019697:Onions / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D016587:Antimutagenic Agents; D009153:Mutagens; D007444:Inulin; D008741:Methyl Methanesulfonate