title: Proteomic analysis of the somatic and surface compartments from Dirofilaria immitis adult worms.
abstract: Dirofilaria immitis (hearthworm) is a filarial roundworm transmitted by mosquitoes to different vertebrate hosts (dogs, cats and humans, among others), causing dirofilariosis. The adult worms reside in the pulmonary arteries affecting vessels and tissues and resulting in different pathological manifestations. Worms migrate to the heart and surrounding major vessels in heavy infections. Dirofilariosis can result in serious damage to affected hosts. In the last few years, a re-emergence of the disease driven by the climate change has been pointed out. Very recently, the knowledge at molecular level of this parasite has been extended by the published studies on its genome and transcriptome. Nevertheless, studies on the expression of defined protein sets in different parasite compartments and the corresponding role of those proteins in the host-parasite relationship have been relatively scarce to date. These include the description of the adult worm secretome, and some of the proteins eliciting humoural immune responses and those related with plasminogen binding in secreted and surface extracts of the parasite. Here, we investigate by proteomics the somatic and surface compartments of the D. immitis adult worm, adding new information on protein expression and localization that would facilitate a deeper understanding of the host-parasite relationships in dirofilariosis.
authors.affiliation: Laboratory of Parasitology, Faculty of Pharmacy, University of Salamanca, Avda, Licenciado Méndez Nieto, s/n, 37007 Salamanca, Spain. Electronic address: rmorgar@usal.es.
authors.forename: R
authors.initials: R
authors.lastname: Morchón
authors.affiliation: Laboratory of Parasitology, Faculty of Pharmacy, University of Salamanca, Avda, Licenciado Méndez Nieto, s/n, 37007 Salamanca, Spain.
authors.forename: J
authors.initials: J
authors.lastname: González-Miguel
authors.affiliation: Department of Animal Medicine and Surgery, Veterinary Faculty, Universidad de Las Palmas de Gran Canaria, Canary Islands, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Carretón
authors.affiliation: Department of Animal Production, Veterinary Biotechnology and Food Safety, University of Parma, Via del Taglio 8, 43100 Parma, Italy.
authors.forename: L H
authors.initials: LH
authors.lastname: Kramer
authors.affiliation: Servicio Central de Apoyo a la Investigación Experimental, Universidad de Valencia, C/Dr. Moliner, 50, 46100 Burjasot, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Valero
authors.affiliation: Department of Animal Medicine and Surgery, Veterinary Faculty, Universidad de Las Palmas de Gran Canaria, Canary Islands, Spain.
authors.forename: J A
authors.initials: JA
authors.lastname: Montoya-Alonso
authors.affiliation: Laboratory of Parasitology, Faculty of Pharmacy, University of Salamanca, Avda, Licenciado Méndez Nieto, s/n, 37007 Salamanca, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Simón
authors.affiliation: IRNASA, CSIC, C/Cordel de Merinas, 40-52, 37008 Salamanca, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Siles-Lucas
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000235:genetics* / Q000378:metabolism*; D004183:Dirofilaria immitis / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D015801:Helminth Proteins / Q000235:genetics* / Q000378:metabolism*; D008297:Male; D021381:Protein Transport; D020543:Proteome; D040901:Proteomics
chemical_list: D000954:Antigens, Surface; D015801:Helminth Proteins; D020543:Proteome
keywords: Dirofilaria immitis; Proteomics; Somatic; Surface