title: Hypoxia augments lipopolysaccharide-induced cytokine expression in periodontal ligament cells.
abstract: Periodontitis is a chronic inflammatory disease characterized by the destruction of tooth supporting tissues. Hypoxia, the mainly changes of the plateau environment, can induce severe periodontitis by animal experiments. There is, however, very little information on hypoxia and lipopolysaccharide (LPS) induced cytokine expression in periodontal ligament (PDL) cells. In this article, we characterized hypoxia or P. gingivalis lipopolysaccharide (Pg LPS) induced tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, and IL-6 expression by human periodontal ligament (hPDL) cells. We found that hypoxia augmented Pg LPS induced TNF-α, IL-1β, and IL-6 expression in hPDL cells. We also demonstrated that nuclear factor kappa B pathway was involved in hypoxia augmenting Pg LPS induced cytokine expression in hPDL cells. Thus, our results suggest that the hypoxic environment may enhance the immune function of hPDL cells that is induced by Pg LPS.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Second Affiliated Hospital, Third Military Medical University, Xinqiaozheng Street, Shapingba District, Chongqing, 400038, People's Republic of China.
authors.forename: Congxiang
authors.initials: C
authors.lastname: Jian
authors.forename: Chenjun
authors.initials: C
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Ren
authors.forename: Yong
authors.initials: Y
authors.lastname: He
authors.forename: Yunming
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Feng
authors.forename: Gang
authors.initials: G
authors.lastname: Zhang
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Tan
mesh_terms: D000293:Adolescent; D015687:Cell Hypoxia / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides / Q000633:toxicity*; D010513:Periodontal Ligament / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D055815:Young Adult
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides