title: Effective antifouling using quorum-quenching acylase stabilized in magnetically-separable mesoporous silica.
abstract: Highly effective antifouling was achieved by immobilizing and stabilizing an acylase, disrupting bacterial cell-to-cell communication, in the form of cross-linked enzymes in magnetically separable mesoporous silica. This so-called "quorum-quenching" acylase (AC) was adsorbed into spherical mesoporous silica (S-MPS) with magnetic nanoparticles (Mag-S-MPS), and further cross-linked for the preparation of nanoscale enzyme reactors of AC in Mag-S-MPS (NER-AC/Mag-S-MPS). NER-AC effectively stabilized the AC activity under rigorous shaking at 200 rpm for 1 month, while free and adsorbed AC lost more than 90% of their initial activities in the same condition within 1 and 10 days, respectively. When applied to the membrane filtration for advanced water treatment, NER-AC efficiently alleviated the biofilm maturation of Pseudomonas aeruginosa PAO1 on the membrane surface, thereby enhancing the filtration performance by preventing membrane fouling. Highly stable and magnetically separable NER-AC, as an effective and sustainable antifouling material, has a great potential to be used in the membrane filtration for water reclamation.
authors.affiliation: Department of Chemical and Biological Engineering, Korea University , Seoul 136-701, Korea.
authors.forename: Byoungsoo
authors.initials: B
authors.lastname: Lee
authors.forename: Kyung-Min
authors.initials: KM
authors.lastname: Yeon
authors.forename: Jongmin
authors.initials: J
authors.lastname: Shim
authors.forename: Sang-Ryoung
authors.initials: SR
authors.lastname: Kim
authors.forename: Chung-Hak
authors.initials: CH
authors.lastname: Lee
authors.forename: Jinwoo
authors.initials: J
authors.lastname: Lee
authors.forename: Jungbae
authors.initials: J
authors.lastname: Kim
mesh_terms: D000581:Amidohydrolases / Q000737:chemistry* / Q000378:metabolism*; D018441:Biofilms; D057219:Biofouling / Q000517:prevention & control*; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005374:Filtration / Q000295:instrumentation / Q000379:methods; D058185:Magnetite Nanoparticles; D008567:Membranes, Artificial; D011550:Pseudomonas aeruginosa / Q000502:physiology; D053038:Quorum Sensing; D012822:Silicon Dioxide; D018508:Water Purification / Q000295:instrumentation / Q000379:methods
chemical_list: D004800:Enzymes, Immobilized; D058185:Magnetite Nanoparticles; D008567:Membranes, Artificial; D012822:Silicon Dioxide; D000581:Amidohydrolases; C019665:amidase