title: Transcription factor GATA1 is dispensable for mast cell differentiation in adult mice.
abstract: Although previous studies have shown that GATA1 is required for mast cell differentiation, the effects of the complete ablation of GATA1 in mast cells have not been examined. Using conditional Gata1 knockout mice (Gata1(-/y)), we demonstrate here that the complete ablation of GATA1 has a minimal effect on the number and distribution of peripheral tissue mast cells in adult mice. The Gata1(-/y) bone marrow cells were capable of differentiating into mast cells ex vivo. Microarray analyses showed that the repression of GATA1 in bone marrow mast cells (BMMCs) has a small impact on the mast cell-specific gene expression in most cases. Interestingly, however, the expression levels of mast cell tryptases in the mouse chromosome 17A3.3 were uniformly reduced in the GATA1 knockdown cells, and GATA1 was found to bind to a 500-bp region at the 5' end of this locus. Revealing a sharp contrast to that observed in the Gata1-null BMMCs, GATA2 deficiency resulted in a significant loss of the c-Kit(+) FcεRIα(+) mast cell fraction and a reduced expression of several mast cell-specific genes. Collectively, GATA2 plays a more important role than GATA1 in the regulation of most mast cell-specific genes, while GATA1 might play specific roles in mast cell functions.
authors.affiliation: Department of Pharmacy, Faculty of Pharmacy, Takasaki University of Health and Welfare, Takasaki, Gunma, Japan.
authors.forename: Kinuko
authors.initials: K
authors.lastname: Ohneda
authors.forename: Takashi
authors.initials: T
authors.lastname: Moriguchi
authors.forename: Shin'ya
authors.initials: S
authors.lastname: Ohmori
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ishijima
authors.forename: Hironori
authors.initials: H
authors.lastname: Satoh
authors.forename: Sjaak
authors.initials: S
authors.lastname: Philipsen
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D001483:Base Sequence; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D033481:Chromosomes, Mammalian; D003470:Culture Media; D050982:GATA1 Transcription Factor / Q000502:physiology*; D050989:GATA2 Transcription Factor / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic; D008297:Male; D008407:Mast Cells / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D059467:Transcriptome; D053802:Tryptases / Q000235:genetics / Q000378:metabolism
chemical_list: D003470:Culture Media; D050982:GATA1 Transcription Factor; D050989:GATA2 Transcription Factor; C494689:Gata1 protein, mouse; C494712:Gata2 protein, mouse; D053802:Tryptases