title: Effect of feeding garlic leaf on microbial nitrogen supply, kinetics of plasma phenylalanine, tyrosine and protein synthesis in sheep.
abstract: The objective of the present study was to assess the feeding effects of garlic leaf on microbial N supply (MNS), turnover rates of plasma phenylalanine (PheTR) and tyrosine (TyrTR) and whole body protein synthesis (WBPS) in sheep. The sheep were fed either mixed hay (Hay-diet, as control) or hay plus garlic leaf diet (GL-diet, at a ratio of 9:1) in a crossover design each for a 21 day period. The isotope dilution method using [(2) H5 ]Phe and [(2) H2 ]Tyr was performed on the 21st day of each dietary treatment. Nitrogen intake remained similar between the diets and N absorption and N digestibility were higher (P<0.05) in the GL-diet than Hay-diet. Total purine derivatives excretion and MNS were greater (P<0.05) in the GL-diet than the Hay-diet. Plasma PheTR tended to be higher (P=0.06) during GL feeding and TyrTR did not differ between the diets. Further, WBPS tended to be greater (P=0.05) for the GL-diet compared with the Hay-diet. Hence, the present results suggest that garlic leaf may have positive effects on N metabolism by influencing MNS in sheep and could be used as a potential ruminant feed in the future.
authors.affiliation: Department of Animal Sciences, Faculty of Agriculture, Iwate University, Morioka, Japan.
authors.forename: Md
authors.initials: M
authors.lastname: Kamruzzaman
authors.forename: Xi
authors.initials: X
authors.lastname: Liang
authors.forename: Natsumi
authors.initials: N
authors.lastname: Sekiguchi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Sano
mesh_terms: D000821:Animal Feed; D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D018592:Cross-Over Studies; D005737:Garlic; D007700:Kinetics; D008297:Male; D009584:Nitrogen / Q000378:metabolism*; D010649:Phenylalanine / Q000097:blood*; D018515:Plant Leaves; D014176:Protein Biosynthesis / Q000502:physiology*; D012756:Sheep / Q000378:metabolism* / Q000382:microbiology; D014443:Tyrosine / Q000097:blood*
chemical_list: D014443:Tyrosine; D010649:Phenylalanine; D009584:Nitrogen
keywords: [2H2]tyrosine; [2H5]phenylalanine; garlic leaf; protein synthesis; sheep