title: Dendritic cells pulsed with leukemia cell-derived exosomes more efficiently induce antileukemic immunities.
abstract: Dendritic cells (DCs) and tumor cell-derived exosomes have been used to develop antitumor vaccines. However, the biological properties and antileukemic effects of leukemia cell-derived exosomes (LEXs) are not well described. In this study, the biological properties and induction of antileukemic immunity of LEXs were investigated using transmission electron microscopy, western blot analysis, cytotoxicity assays, and animal studies. Similar to other tumor cells, leukemia cells release exosomes. Exosomes derived from K562 leukemia cells (LEXK562) are membrane-bound vesicles with diameters of approximately 50-100 μm and harbor adhesion molecules (e.g., intercellular adhesion molecule-1) and immunologically associated molecules (e.g., heat shock protein 70). In cytotoxicity assays and animal studies, LEXs-pulsed DCs induced an antileukemic cytotoxic T-lymphocyte immune response and antileukemic immunity more effectively than did LEXs and non-pulsed DCs (P<0.05). Therefore, LEXs may harbor antigens and immunological molecules associated with leukemia cells. As such, LEX-based vaccines may be a promising strategy for prolonging disease-free survival in patients with leukemia after chemotherapy or hematopoietic stem cell transplantation.
authors.affiliation: Department of Hematology, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Department of Hematology, The First People's Hospital of Shanghai Affiliated to Shanghai Jiaotong University, Shanghai, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Hematology, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: Department of Hematology, The First People's Hospital of Shanghai Affiliated to Shanghai Jiaotong University, Shanghai, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Shen
authors.affiliation: Department of Hematology, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Deng
authors.affiliation: Department of Hematology, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: Linjun
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Hematology, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Hematology, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: Siguo
authors.initials: S
authors.lastname: Hao
mesh_terms: D000818:Animals; D001692:Biological Transport; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology* / Q000648:ultrastructure; D055354:Exosomes / Q000473:pathology*; D005260:Female; D006801:Humans; D020014:K562 Cells; D007938:Leukemia / Q000276:immunology* / Q000473:pathology*; D051379:Mice; D046529:Microscopy, Electron, Transmission; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology