title: Accessing transcriptomic data for ecologically important genes in the goose barnacle (Pollicipes pollicipes), with particular focus on cement proteins.
abstract: In this study 4310 expressed sequence tags (ESTs) were used to identify potentially useful transcripts for future studies in the gooseneck barnacle Pollicipes pollicipes (Gmelin, 1789). 119 ESTs were obtained in this work and 4191 were taken from Meusemann et al. (2010). The gooseneck barnacle is a sessile pedunculate cirripede of great economic importance that occurs in dense aggregations, and is harvested for human consumption. The assembly of these ESTs yielded 1805 unigenes (461 contigs and 1344 singlets). The identification of cement proteins in our data is particularly interesting for cirripedes. Only a small part of the assembled unigenes could be functionally annotated. However, our results greatly improve our understanding of the biological features of P. pollicipes. In addition to this, a large number of potentially interesting genes were identified in order to serve as the base for future evolutionary studies in P. pollicipes.
authors.affiliation: Department of Cell and Molecular Biology, Evolutionary Biology Group (GIBE), Universidade da Coruña, A Fraga 10, E-15008 A Coruña, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Perina
authors.affiliation: Department of Life Sciences, The Natural History Museum, London, United Kingdom.
authors.forename: B M
authors.initials: BM
authors.lastname: von Reumont
authors.affiliation: Department of Cell and Molecular Biology, Evolutionary Biology Group (GIBE), Universidade da Coruña, A Fraga 10, E-15008 A Coruña, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Martínez-Lage
authors.affiliation: Department of Cell and Molecular Biology, Evolutionary Biology Group (GIBE), Universidade da Coruña, A Fraga 10, E-15008 A Coruña, Spain. Electronic address: hakuna@udc.es.
authors.forename: A M
authors.initials: AM
authors.lastname: González-Tizón
mesh_terms: D000818:Animals; D001483:Base Sequence; D016000:Cluster Analysis; D019295:Computational Biology; D020224:Expressed Sequence Tags; D015723:Gene Library; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013030:Spain; D001468:Thoracica / Q000235:genetics* / Q000378:metabolism*; D059467:Transcriptome / Q000235:genetics*
chemical_list: D011506:Proteins
keywords: Annotation; Cement proteins; Expressed sequence tags (ESTs); Pollicipes pollicipes