title: Lactobacillus brevis strains from fermented aloe vera survive gastroduodenal environment and suppress common food borne enteropathogens.
abstract: Five novel Lactobacillus brevis strains were isolated from naturally fermented Aloe vera leaf flesh. Each strain was identified by Random Amplified Polymorphic DNA (RAPD) analysis and 16S rRNA sequence comparison. These strains were highly tolerant to acid, surviving in pH2.5 for up to 4 hours, and resistant to 5% bile salts at 37°C for 18 hours. Due to its tolerance to acid and bile salts, one strain passed through the gastric barrier and colonised the intestine after oral administration. All five strains inhibited the growth of many harmful enteropathogens without restraining most of normal commensals in the gut and hence named POAL (Probiotics Originating from Aloe Leaf) strains. Additionally, each strain exhibited discriminative resistance to a wide range of antibiotics. The L. brevis POAL strains, moreover, expressed high levels of the glutamate decarboxylase (GAD) gene which produces a beneficial neurotransmitter, γ-aminobutyric acid (GABA). These characteristics in all suggest that the novel L. brevis strains should be considered as potential food additives and resources for pharmaceutical research.
authors.affiliation: KBNP Technology Institute, KBNP Inc., Yesan, Korea.
authors.forename: Young-Wook
authors.initials: YW
authors.lastname: Kim
authors.affiliation: KBNP Technology Institute, KBNP Inc., Yesan, Korea.
authors.forename: Young-Ju
authors.initials: YJ
authors.lastname: Jeong
authors.affiliation: School of Biological Sciences and Technology, Chonnam National University, Gwangju, Korea.
authors.forename: Ah-Young
authors.initials: AY
authors.lastname: Kim
authors.affiliation: KBNP Technology Institute, KBNP Inc., Yesan, Korea.
authors.forename: Hyun-Hee
authors.initials: HH
authors.lastname: Son
authors.affiliation: KBNP Technology Institute, KBNP Inc., Yesan, Korea.
authors.forename: Jong-Am
authors.initials: JA
authors.lastname: Lee
authors.affiliation: KBNP Technology Institute, KBNP Inc., Yesan, Korea.
authors.forename: Cheong-Hwan
authors.initials: CH
authors.lastname: Jung
authors.affiliation: KBNP Technology Institute, KBNP Inc., Yesan, Korea.
authors.forename: Chae-Hyun
authors.initials: CH
authors.lastname: Kim
authors.affiliation: Department of Biology, Mokpo National University, Muan, Korea.
authors.forename: Jaeman
authors.initials: J
authors.lastname: Kim
mesh_terms: D000504:Aloe / Q000382:microbiology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D001647:Bile Acids and Salts / Q000494:pharmacology; D004386:Duodenum / Q000382:microbiology*; D005285:Fermentation / Q000187:drug effects; D005516:Food Microbiology; D005968:Glutamate Decarboxylase / Q000235:genetics / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D052196:Levilactobacillus brevis / Q000187:drug effects / Q000302:isolation & purification*; D051379:Mice; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D008969:Molecular Sequence Data; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology; D019936:Probiotics / Q000494:pharmacology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013270:Stomach / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D001647:Bile Acids and Salts; D012336:RNA, Ribosomal, 16S; D005968:Glutamate Decarboxylase