title: [Phylogenetic analysis of genomes of Vibrio cholerae strains isolated on the territory of Rostov region].
abstract: OBJECTIVE
  Determination of origin of 2 Vibrio cholerae strains isolated on the territory of Rostov region by using full genome sequencing data.
  
  
  METHODS
  Toxigenic strain 2011 EL- 301 V. cholerae 01 El Tor Inaba No. 301 (ctxAB+, tcpA+) and nontoxigenic strain V. cholerae O1 Ogawa P- 18785 (ctxAB-, tcpA+) were studied. Sequencing was carried out on the MiSeq platform. Phylogenetic analysis of the genomes obtained was carried out based on comparison of conservative part of the studied and 54 previously sequenced genomes.
  
  
  RESULTS
  2011EL-301 strain genome was presented by 164 contigs with an average coverage of 100, N50 parameter was 132 kb, for strain P- 18785 - 159 contigs with a coverage of69, N50 - 83 kb. The contigs obtained for strain 2011 EL-301 were deposited in DDBJ/EMBL/GenBank databases with access code AJFN02000000, for strain P-18785 - ANHS00000000. 716 protein-coding orthologous genes were detected. Based on phylogenetic analysis strain P- 18785 belongs to PG-1 subgroup (a group of predecessor strains of the 7th pandemic). Strain 2011EL-301 belongs to groups of strains of the 7th pandemic and is included into the cluster with later isolates that are associated with cases of cholera in South Africa and cases of import of cholera to the USA from Pakistan.
  
  
  CONCLUSIONS
  The data obtained allows to establish phylogenetic connections with V cholerae strains isolated earlier.
authors.forename: K V
authors.initials: KV
authors.lastname: Kuleshov
authors.forename: M L
authors.initials: ML
authors.lastname: Markelov
authors.forename: V G
authors.initials: VG
authors.lastname: Dedkov
authors.forename: A S
authors.initials: AS
authors.lastname: Vodop'ianov
authors.forename: A V
authors.initials: AV
authors.lastname: Kermanov
authors.forename: R V
authors.initials: RV
authors.lastname: Pisanov
authors.forename: V D
authors.initials: VD
authors.lastname: Kruglikov
authors.forename: A B
authors.initials: AB
authors.lastname: Mazrukho
authors.forename: V V
authors.initials: VV
authors.lastname: Maleev
authors.forename: G A
authors.initials: GA
authors.lastname: Shipulin
mesh_terms: D001483:Base Sequence; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D010802:Phylogeny; D012426:Russia; D017422:Sequence Analysis, DNA; D014734:Vibrio cholerae / Q000235:genetics* / Q000302:isolation & purification