title: Peptide-directed self-assembly of functionalized polymeric nanoparticles part I: design and self-assembly of peptide-copolymer conjugates into nanoparticle fibers and 3D scaffolds.
abstract: A robust self-assembly of nanoparticles into fibers and 3D scaffolds is designed and fabricated by functionalizing a RAFT-polymerized amphiphilic triblock copolymer with designer ionic complementary peptides so that the assembled core-shell polymeric nanoparticles are directed by peptide assembly into continuous "nanoparticle fibers," ultimately leading to 3D fiber scaffolds. The assembled nanostructure is confirmed by FESEM and optical microscopy. The assembly is not hindered when a protein (insulin) is incorporated within the nanoparticles as an active ingredient. MTS cytotoxicity tests on SW-620 cell lines show that the peptides, copolymers, and peptide-copolymer conjugates are biocompatible. The methodology of self-assembled nanoparticle fibers and 3D scaffolds is intended to combine the advantages of a flexible hydrogel scaffold with the versatility of controlled release nanoparticles to offer unprecedented ability to incorporate desired drug(s) within a self-assembled scaffold system with individual control over the release of each drug.
authors.affiliation: Department of Chemistry, Michigan Technological University, Houghton, MI, 49931, USA. xding@mtu.edu.
authors.forename: Xiaochu
authors.initials: X
authors.lastname: Ding
authors.forename: Jagadeesh
authors.initials: J
authors.lastname: Janjanam
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Tiwari
authors.forename: Martin
authors.initials: M
authors.lastname: Thompson
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Heiden
mesh_terms: D002460:Cell Line; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000494:pharmacology; D007328:Insulin / Q000737:chemistry / Q000494:pharmacology; D008422:Materials Testing; D057139:Nanofibers / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*
chemical_list: D003692:Delayed-Action Preparations; D007004:Hypoglycemic Agents; D007328:Insulin; D010455:Peptides
keywords: RAFT polymerization; drug delivery systems; nanoparticles; peptide self-assembly; scaffolds