title: Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in rats.
abstract: OBJECTIVE
  To investigate the effects of alloxan diabetes on the abdominal wall healing of rats undergoing laparotomy.
  
  
  METHODS
  Ninety-six male Wistar rats weighing between 200 and 300 grams, divided into two groups: non-diabetic group (G1) and another with untreated diabetes (G2). Three months after diabetes induction, the animals underwent a 5cm-long- laparotomy and 5.0 nylon monofilament suture. After the surgery, 12 animals from each group were euthanized on days 4, 14, 21 and 30 corresponding to the moments M1, M2, M3 and M4. In each moment a fragment of the abdominal wall containing the scar was removed for tensile strength measurement, histological and morphometric study. Clinical and biochemical parameters were also analyzed.
  
  
  RESULTS
  G2 animals showed parameters compatible with severe diabetes and decreased plasma levels of insulin. The tensile strength in G2 was significantly smaller in M2 and M4, with a tendency to fall in the other two. Through light microscope, diabetic animals showed more difficulty to increase collagen density and contraction. G2 animals showed high cellularity of fibroblasts in later healing moments, with collagen thinning in M2 and M4.
  
  
  CONCLUSIONS
  The abdominal wound healing in untreated diabetic animals was altered and led to a higher incidence of dehiscence and infections.
authors.affiliation: Sao Paulo State University, Faculty of Medicine, Departament of Surgery and Orthopedics, BotucatuSP, Brazil, PhD, Assistant Professor, Departament of Surgery and Orthopedics, Faculty of Medicine, Sao Paulo State University (UNESP), Botucatu-SP, Brazil. Conception, design, intellectual and scientific content of the study; acquisition and interpretation of data; technical procedures; manuscript writing.
authors.forename: José Guilherme
authors.initials: JG
authors.lastname: Minossi
authors.affiliation: UNESP, Faculty of Medicine, Department of Pathology, BotucatuSP, Brazil, PhD, Assistant Professor, Department of Pathology, Faculty of Medicine, UNESP, Botucatu-SP, Brazil. Histophatological analysis.
authors.forename: Flávio de Oliveira
authors.initials: Fde O
authors.lastname: Lima
authors.affiliation: UNESP, Faculty of Medicine, Department of Medical Clinic, BotucatuSP, Brazil, PhD, Associate Professor, Department of Medical Clinic, Faculty of Medicine, UNESP, Botucatu-SP, Brazil. Morphometric analysis.
authors.forename: Carlos Antonio
authors.initials: CA
authors.lastname: Caramori
authors.affiliation: UNESP, Faculty of Medicine, BotucatuSP, Brazil, Fellow Master degree, Postgraduate Program in General Basis of Surgery, Faculty of Medicine, UNESP, Botucatu-SP, Brazil. Technical procedures, interpretation of data.
authors.forename: Claudia Nishida
authors.initials: CN
authors.lastname: Hasimoto
authors.affiliation: UNESP, Faculty of Medicine, Department of Surgery and Orthopedics, BotucatuSP, Brazil, PhD, Assistant Professor, Department of Surgery and Orthopedics, Faculty of Medicine, UNESP, Botucatu-SP, Brazil. Acquisition and interpretation of data.
authors.forename: Erica Veruska Paiva
authors.initials: EV
authors.lastname: Ortolan
authors.affiliation: UNESP, Faculty of Medicine, Department of Surgery and Orthopedics, BotucatuSP, Brazil, PhD, Assistant Professor, Departament of Surgery and Orthopedics, Faculty of Medicine, UNESP, Botucatu-SP, Brazil. Acquisition of data, manuscript writing.
authors.forename: Paulo Antônio
authors.initials: PA
authors.lastname: Rodrigues
authors.affiliation: UNESP, Faculty of Medicine, Department of Surgery and Orthopedics, BotucatuSP, Brazil, PhD, Full Professor, Department of Surgery and Orthopedics, Faculty of Medicine, UNESP, Botucatu-SP, Brazil. Critical revision.
authors.forename: César Tadeu
authors.initials: CT
authors.lastname: Spadella
mesh_terms: D034861:Abdominal Wall / Q000033:anatomy & histology / Q000503:physiopathology* / Q000601:surgery; D000496:Alloxan; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002921:Cicatrix; D003094:Collagen / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000503:physiopathology*; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000502:physiology; D007328:Insulin / Q000097:blood; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013718:Tensile Strength / Q000502:physiology*; D013997:Time Factors; D014945:Wound Healing / Q000502:physiology*
chemical_list: D001786:Blood Glucose; D007328:Insulin; D000496:Alloxan; D003094:Collagen