title: Four cases of Taenia saginata infection with an analysis of COX1 gene.
abstract: Human taeniases had been not uncommon in the Republic of Korea (=Korea) until the 1980s. The prevalence decreased and a national survey in 2004 revealed no Taenia egg positive cases. However, a subsequent national survey in 2012 showed 0.04% (10 cases) prevalence of Taenia spp. eggs suggesting its resurgence in Korea. We recently encountered 4 cases of Taenia saginata infection who had symptoms of taeniasis that included discharge of proglottids. We obtained several proglottids from each case. Because the morphological features of T. saginata are almost indistinguishable from those of Taenia asiatica, molecular analyses using the PCR-RFLP and DNA sequencing of the cytochrome c oxidase subunit 1 (cox1) were performed to identify the species. The PCR-RFLP patterns of all of the 4 specimens were consistent with T. saginata, and the cox1 gene sequence showed 99.8-100% identity with that of T. saginata reported previously from Korea, Japan, China, and Cambodia. All of the 4 patients had the history of travel abroad but its relation with contracting taeniasis was unclear. Our findings may suggest resurgence of T. saginata infection among people in Korea.
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea.
authors.forename: Jaeeun
authors.initials: J
authors.lastname: Cho
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea.
authors.forename: Bong-Kwang
authors.initials: BK
authors.lastname: Jung
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea.
authors.forename: Hyemi
authors.initials: H
authors.lastname: Lim
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea. ; Department of Internal Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea.
authors.forename: Min-Jae
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Department of Biology, Faculty of Science, Thaksin University, Phatthalung 93110, Thailand.
authors.forename: Thanapon
authors.initials: T
authors.lastname: Yooyen
authors.affiliation: Department of Parasitology, Medical Research Institute and Parasite Resource Bank, Chungbuk National University School of Medicine, Cheongju 361-763, Korea.
authors.forename: Dongmin
authors.initials: D
authors.lastname: Lee
authors.affiliation: Department of Parasitology, Medical Research Institute and Parasite Resource Bank, Chungbuk National University School of Medicine, Cheongju 361-763, Korea.
authors.forename: Keeseon S
authors.initials: KS
authors.lastname: Eom
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea. ; Seoul National University Bundang Hospital, Seongnam 463-707, Korea.
authors.forename: Eun-Hee
authors.initials: EH
authors.lastname: Shin
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea.
authors.forename: Jong-Yil
authors.initials: JY
authors.lastname: Chai
mesh_terms: D000328:Adult; D000818:Animals; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D003576:Electron Transport Complex IV / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D041202:Taenia saginata / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D013622:Taeniasis / Q000175:diagnosis* / Q000469:parasitology*; D014195:Travel
chemical_list: D003576:Electron Transport Complex IV
keywords: PCR-RFLP; Taenia saginata; case report; cox1; molecular diagnosis; sequence divergence