title: [Inhibition effect of 6-gingerol on hair growth].
abstract: OBJECTIVE
  To investigate the effect of 6-gingerol, the main active component of ginger, on hair shaft elongation in vitro and hair growth in vivo.
  
  
  METHODS
  Firstly, Hair follicles were co-cultured with 3 different concentration of 6-gingerol for 5 days and hair elongation in three groups was measured. Secondly, The proliferative effect of 6-gingerol on DPCs was measured using MTT assay. Thirdly, the expression of Bcl-2 and Bax in DPCs were measured using Western blotting. In vivo study, the influence of 6-gingerol on hair growth in C57BL/6 rats was measured through topical application of 6-gingerol on the dorsal skin of each animal.
  
  
  RESULTS
  The length of hair shaft in 20 microg/ml 6-Gingerol group (0.50 +/- 0.08 mm) is less than 0 microg/ml (0.66 +/- 0.19) mm and 10 microg/ml (0.64 +/- 0.03) mm 6-Gingerol group (P < 0.05). In cell culture, compared to 0 microg/ml and 5 microg/ml 6-Gingerol, 10 microg/ml 6-Gingerol can significantly inhibited the proliferation of DPCs (P < 0.05). Along with the growth inhibition of DPCs by 6-gingerol, the Bax/Bcl-2 ratio increased obviously. In vivo study, the hair length and density decreased a lot after using 1 mg/ml 6-gingerol.
  
  
  CONCLUSIONS
  6-Gingerol can suppress human hair shaft elongation because it has pro-apoptotic effects on DPCs via increasing Bax/Bcl-2 ratio. It might inhibit hair growth by prolonging the telogen stage in vivo.
authors.forename: Yong
authors.initials: Y
authors.lastname: Miao
authors.forename: Ya-Bin
authors.initials: YB
authors.lastname: Sun
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Wang
authors.forename: Zhi-Dan
authors.initials: ZD
authors.lastname: Zhang
authors.forename: Jin-Dou
authors.initials: JD
authors.lastname: Jiang
authors.forename: Ze-Hua
authors.initials: ZH
authors.lastname: Li
authors.forename: Zhi-Qi
authors.initials: ZQ
authors.lastname: Hu
mesh_terms: D000818:Animals; D002396:Catechols / Q000494:pharmacology*; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D005233:Fatty Alcohols / Q000494:pharmacology*; D006197:Hair / Q000187:drug effects* / Q000254:growth & development; D018859:Hair Follicle / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D002396:Catechols; D005233:Fatty Alcohols; D010936:Plant Extracts; D051028:bcl-2-Associated X Protein; C007845:gingerol