title: Dual-responsive polymer-coated iron oxide nanoparticles for drug delivery and imaging applications.
abstract: We reported the synthesis and characterization of dual-responsive poly(N-isopropylacrylamide-acrylamide-chitosan) (PAC)-coated magnetic nanoparticles (MNPs) for controlled and targeted drug delivery and imaging applications. The PAC-MNPs size was about 150nm with 70% iron mass content and excellent superparamagnetic properties. PAC-MNPs loaded with anti-cancer drug doxorubicin showed dual-responsive drug release characteristics with the maximum release of drugs at 40°C (∼78%) than at 37°C (∼33%) and at pH of 6 (∼55%) than at pH of 7.4 (∼28%) after 21 days. Further, the conjugation of prostate cancer-specific R11 peptides increased the uptake of PAC-MNPs by prostate cancer PC3 cells. The dose-dependent cellular uptake of the nanoparticles was also significantly increased with the presence of 1.3T magnetic field. The nanoparticles demonstrated cytocompatibility up to concentrations of 500μg/ml when incubated over a period of 24h with human dermal fibroblasts and normal prostate epithelial cells. Finally, pharmacokinetic studies indicated that doxorubicin-loaded PAC-MNPs caused significant prostate cancer cell death at 40°C than at 37°C, thereby confirming the temperature-dependent drug release kinetics and in vitro therapeutic efficacy. Future evaluation of in vivo therapeutic efficacy of targeted image-guided cancer therapy using R11-PAC-MNPs will reinforce a significant impact of the multifunctional PAC-MNPs on the future drug delivery systems.
authors.affiliation: Department of Bioengineering, The University of Texas at Arlington, Arlington, TX 76019, United States; Joint Biomedical Engineering Program between The University of Texas at Arlington and The University of Texas Southwestern Medical Center, Dallas, TX 75390, United States.
authors.forename: Varsha
authors.initials: V
authors.lastname: Sundaresan
authors.affiliation: Department of Bioengineering, The University of Texas at Arlington, Arlington, TX 76019, United States; Joint Biomedical Engineering Program between The University of Texas at Arlington and The University of Texas Southwestern Medical Center, Dallas, TX 75390, United States.
authors.forename: Jyothi U
authors.initials: JU
authors.lastname: Menon
authors.affiliation: Department of Vascular Surgery, University of Cincinnati, OH 45267, United States.
authors.forename: Maham
authors.initials: M
authors.lastname: Rahimi
authors.affiliation: Department of Bioengineering, The University of Texas at Arlington, Arlington, TX 76019, United States; Joint Biomedical Engineering Program between The University of Texas at Arlington and The University of Texas Southwestern Medical Center, Dallas, TX 75390, United States.
authors.forename: Kytai T
authors.initials: KT
authors.lastname: Nguyen
authors.affiliation: Department of Bioengineering, The University of Texas at Arlington, Arlington, TX 76019, United States; Joint Biomedical Engineering Program between The University of Texas at Arlington and The University of Texas Southwestern Medical Center, Dallas, TX 75390, United States. Electronic address: aniketwadajkar@yahoo.com.
authors.forename: Aniket S
authors.initials: AS
authors.lastname: Wadajkar
mesh_terms: D000180:Acrylic Resins / Q000008:administration & dosage / Q000737:chemistry*; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage / Q000737:chemistry; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D048271:Chitosan / Q000008:administration & dosage / Q000737:chemistry*; D003952:Diagnostic Imaging; D004317:Doxorubicin / Q000008:administration & dosage / Q000737:chemistry; D016503:Drug Delivery Systems; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005290:Ferric Compounds / Q000008:administration & dosage / Q000737:chemistry*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D060328:Magnetic Phenomena; D008297:Male; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D011467:Prostate / Q000166:cytology
chemical_list: D000180:Acrylic Resins; D000903:Antibiotics, Antineoplastic; D005290:Ferric Compounds; C000499:ferric oxide; C052970:poly-N-isopropylacrylamide; D004317:Doxorubicin; D048271:Chitosan
keywords: Chitosan; Dual-responsiveness; Iron oxide magnetic nanoparticles; Poly(N-isopropylacrylamide); Prostate cancer