title: RNA silencing components mediate resistance signaling against turnip crinkle virus.
abstract: Species-specific immunity is induced when an effector protein from a specific pathogen strain is perceived by a cognate resistance (R) protein in the plant. In Arabidopsis, the R protein HRT, which confers resistance to turnip crinkle virus (TCV), is activated upon recognition of the TCV coat-protein (CP), a potent suppressor of host RNA silencing. Recognition by HRT does not require RNA silencing suppressor function of CP and is not associated with the accumulation of TCV-specific small-RNA. However, several components of the host RNA silencing pathway participate in HRT-mediated defense against TCV. For example, the double stranded RNA binding protein (DRB) 4 interacts with the plasma membrane localized HRT, and is required for its stability. Intriguingly, TCV infection promotes the cytosolic accumulation of the otherwise primarily nuclear DRB4, and this in turn inhibits HRT-DRB4 interaction. These data together with differential localization of DRB4 in plants inoculated with avirulent and virulent viruses, suggests that sub-cellular compartmentalization of DRB4 plays an important role in activation of HRT.
authors.affiliation: Current affiliation: Department of Plant Biology and Ecology; College of Life Sciences; Nankai University; Tanjin, PR China.
authors.forename: Shifeng
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Department of Plant Pathology; University of Kentucky; Lexington, KY USA.
authors.forename: Gah-Hyun
authors.initials: GH
authors.lastname: Lim
authors.affiliation: Department of Plant Pathology; University of Kentucky; Lexington, KY USA.
authors.forename: Keshun
authors.initials: K
authors.lastname: Yu
authors.affiliation: Current affiliation: Korea Atomic Energy Research Institute; Jeongeup si Jeonlabukdo, South Korea.
authors.forename: Rae-Dong
authors.initials: RD
authors.lastname: Jeong
authors.affiliation: Department of Plant Pathology; University of Kentucky; Lexington, KY USA.
authors.forename: Aardra
authors.initials: A
authors.lastname: Kachroo
authors.affiliation: Department of Plant Pathology; University of Kentucky; Lexington, KY USA.
authors.forename: Pradeep
authors.initials: P
authors.lastname: Kachroo
mesh_terms: D017360:Arabidopsis / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D029681:Arabidopsis Proteins / Q000378:metabolism*; D019184:Carmovirus / Q000276:immunology*; D054884:Host-Pathogen Interactions; D010935:Plant Diseases / Q000276:immunology; D057865:Plant Immunity; D034622:RNA Interference; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism*; D015678:Viral Structural Proteins / Q000276:immunology
chemical_list: D029681:Arabidopsis Proteins; C511456:DRB4 protein, Arabidopsis; C491954:HRT protein, Arabidopsis; D016601:RNA-Binding Proteins; D012097:Repressor Proteins; D015678:Viral Structural Proteins
keywords: DRB4; RNA silencing; plant defense; resistance protein; turnip crinkle virus