title: A compact microfluidic system for cell migration studies.
abstract: Microfluidic systems can better control cellular microenvironments and therefore are increasingly used for cell migration research. However, most existing systems are impractical to use without specialized facilities and researchers. Toward removing this barrier, we developed a compact USB microscope-based Microfluidic Chemotaxis Analysis System (UMCAS). This system integrates microfluidic devices, live cell imaging, environmental control and data analysis to provide a solution for rapid microfluidic cell migration and chemotaxis experiments with real-time result reporting. This developed system was successfully validated by testing neutrophil chemotaxis.
authors.affiliation: Department of Physics and Astronomy, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada.
authors.forename: Jiandong
authors.initials: J
authors.lastname: Wu
authors.forename: Lipan
authors.initials: L
authors.lastname: Ouyang
authors.forename: Nitin
authors.initials: N
authors.lastname: Wadhawan
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Michael
authors.initials: M
authors.lastname: Zhang
authors.forename: Simon
authors.initials: S
authors.lastname: Liao
authors.forename: David
authors.initials: D
authors.lastname: Levin
authors.forename: Francis
authors.initials: F
authors.lastname: Lin
mesh_terms: D002170:Canada; D002633:Chemotaxis / Q000502:physiology*; D004912:Erythrocytes / Q000737:chemistry / Q000166:cytology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry / Q000166:cytology; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D008853:Microscopy; D015203:Reproducibility of Results