title: Expression level of miR-93 in formalin-fixed paraffin-embedded tissues of breast cancer patients.
abstract: MiR-93 is thought to be an onco-miRNA for its capabilities of enhancing tumor growth. The objective of this study was to evaluate the potential predictive value of miR-93 expression in formalin-fixed paraffin-embedded (FFPE) tissues of breast cancer patients. The expression of miR-93 was examined in 101 breast cancer patients and 40 controls using real-time quantitative PCR. We found that miR-93 was markedly upregulated in breast cancer patients compared with controls (p<0.01). The expression level of miR-93 was significantly correlated with miR-24/378 in breast cancer patients. MiR-93 exhibited great capability of discriminating between cancer patients and cancer-free controls by receiver-operator characteristic (ROC) curve analysis. MiR-93 showed 0.866 AUC (the area under the ROC curve) values. The MiR-93 level was found significantly correlated with breast cancer by univariable logistic regression. These results suggest that overexpression of miR-93 in FFPE tissues may serve as an indispensable source for biomarker discovery and validation in breast cancer patients.
authors.affiliation: 1 School of Basic Medical Science, Wuhan University , Wuhan, People's Republic of China .
authors.forename: Zhao-qun
authors.initials: ZQ
authors.lastname: Deng
authors.forename: Jun
authors.initials: J
authors.lastname: Qian
authors.forename: Feng-qiong
authors.initials: FQ
authors.lastname: Liu
authors.forename: Jiang
authors.initials: J
authors.lastname: Lin
authors.forename: Rui
authors.initials: R
authors.lastname: Shao
authors.forename: Jia-yu
authors.initials: JY
authors.lastname: Yin
authors.forename: Qin
authors.initials: Q
authors.lastname: Tang
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: He
mesh_terms: D000328:Adult; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000378:metabolism; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D017931:DNA Primers; D005260:Female; D005557:Formaldehyde; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D016612:Paraffin Embedding; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; D017931:DNA Primers; C543350:MIRN93 microRNA, human; D035683:MicroRNAs; D005557:Formaldehyde