title: Immunohistochemical characterization of multicentric hepatocholangiocellular adenoma in a pig.
abstract: Three spherical opaque-white tumor nodules were found in close proximity to each other in the liver of a breeding sow, postslaughter, at a veterinary food inspection. The tumor nodules were circumscribed and histologically consisted of discrete hepatocellular and cholangiocellular nests, in association with polygonal-to-oval-shaped cells with slight cellular atypia. Immunohistochemically, all cellular components were negative for carcinoembryonic antigen, but positive for p53. Both cholangiocytes and oval-shaped cells were immunoreactive to anti-cytokeratin antibodies AE1/AE3 and MNF116. In addition, cholangiocytes were exclusively immunoreactive to anti-cytokeratin antibody CAM5.2, and hepatocytes were positive for MNF116 and hepatocyte paraffin 1. All neoplastic cells were positive for the hepatic progenitor cell markers, α-1-fetoprotein, sal-like protein 4, and epithelial cell adhesion molecule. From these results, the present case was diagnosed as hepatocholangiocellular adenoma, arising from epithelial cells of the canals of Hering.
authors.affiliation: Laboratory of Veterinary Pathology (Segawa, Fujii, Ogawa, Takimoto, Hara, Shibutani), Tokyo University of Agriculture and Technology, Tokyo, JapanLaboratory of Veterinary Toxicology (Murakami, Suzuki), Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Risa
authors.initials: R
authors.lastname: Segawa
authors.affiliation: Laboratory of Veterinary Pathology (Segawa, Fujii, Ogawa, Takimoto, Hara, Shibutani), Tokyo University of Agriculture and Technology, Tokyo, JapanLaboratory of Veterinary Toxicology (Murakami, Suzuki), Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Fujii
authors.affiliation: Laboratory of Veterinary Pathology (Segawa, Fujii, Ogawa, Takimoto, Hara, Shibutani), Tokyo University of Agriculture and Technology, Tokyo, JapanLaboratory of Veterinary Toxicology (Murakami, Suzuki), Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ogawa
authors.affiliation: Laboratory of Veterinary Pathology (Segawa, Fujii, Ogawa, Takimoto, Hara, Shibutani), Tokyo University of Agriculture and Technology, Tokyo, JapanLaboratory of Veterinary Toxicology (Murakami, Suzuki), Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Norifumi
authors.initials: N
authors.lastname: Takimoto
authors.affiliation: Laboratory of Veterinary Pathology (Segawa, Fujii, Ogawa, Takimoto, Hara, Shibutani), Tokyo University of Agriculture and Technology, Tokyo, JapanLaboratory of Veterinary Toxicology (Murakami, Suzuki), Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Shintaro
authors.initials: S
authors.lastname: Hara
authors.affiliation: Laboratory of Veterinary Pathology (Segawa, Fujii, Ogawa, Takimoto, Hara, Shibutani), Tokyo University of Agriculture and Technology, Tokyo, JapanLaboratory of Veterinary Toxicology (Murakami, Suzuki), Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Murakami
authors.affiliation: Laboratory of Veterinary Pathology (Segawa, Fujii, Ogawa, Takimoto, Hara, Shibutani), Tokyo University of Agriculture and Technology, Tokyo, JapanLaboratory of Veterinary Toxicology (Murakami, Suzuki), Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Suzuki
authors.affiliation: Laboratory of Veterinary Pathology (Segawa, Fujii, Ogawa, Takimoto, Hara, Shibutani), Tokyo University of Agriculture and Technology, Tokyo, JapanLaboratory of Veterinary Toxicology (Murakami, Suzuki), Tokyo University of Agriculture and Technology, Tokyo, Japan mshibuta@cc.tuat.ac.jp.
authors.forename: Makoto
authors.initials: M
authors.lastname: Shibutani
keywords: Hepatic progenitor cell; hepatocholangiocellular adenoma; pigs