title: Identification of miR-133b and RB1CC1 as independent predictors for biochemical recurrence and potential therapeutic targets for prostate cancer.
abstract: OBJECTIVE
  We aimed to investigate the contribution of microRNA-133b (miR-133b) in prostate cancer cell proliferation, cell cycle, and apoptosis. We also examined expression of miR-133b in prostate cancer tissues, and evaluated the prognostic significance of miR-133b, as well as its target gene RB1CC1 in patients with prostate cancer after radical prostatectomy.
  
  
  METHODS
  miR-133b mimics (miR-133bm) and anti-miR-133b were transfected into LNCaP and PC-3 cells. CCK-8 was used to look at cell proliferation, flow cytometric analysis was carried out to study cell cycle, and apoptosis was determined by caspase-3 activity. miR-133b expression was assessed by real-time reverse transcription PCR and in situ hybridization in prostatic cell lines and 178 prostate tissue samples, respectively. The protein level of RB1CC1 was examined by Western blot and immunohistochemistry in prostatic cell lines and prostate tissue samples, respectively.
  
  
  RESULTS
  Overexpression of miR-133b in LNCaP cells boosted cell proliferation and cell-cycle progression, but inhibited apoptosis; in contrast, miR-133bm promoted cell apoptosis, but suppressed cell proliferation and cell-cycle progression in PC-3 cells. In LNCaP cells, silencing of RB1CC1, a target of miR-133b, inhibited cell apoptosis, and promoted cell-cycle progression. Moreover, miR-133b expression was significantly inversely correlated with RB1CC1 expression in prostate cancer tissues. Multivariate Cox analysis indicated that miR-133b and RB1CC1 might be two independent prognostic factors of biochemical recurrence.
  
  
  CONCLUSIONS
  miR-133b might enhance tumor-promoting properties in less aggressive LNCaP cells, whereas this miR may act as a tumor suppressor in more aggressive PC-3 cells. miR-133b and RB1CC1 were independent prognostic indicators for prostate cancer.
authors.affiliation: Authors' Affiliations: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University; Department of Urology, Tongji Hospital, Tongji University School of Medicine, Shanghai; and Department of Urology, Hefei First People's Hospital, Hefei, Anhui, People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.forename: Xuechao
authors.initials: X
authors.lastname: Wan
authors.forename: Hongbing
authors.initials: H
authors.lastname: Chen
authors.forename: Shu
authors.initials: S
authors.lastname: Yang
authors.forename: Yiyang
authors.initials: Y
authors.lastname: Liu
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Mo
authors.forename: Delong
authors.initials: D
authors.lastname: Meng
authors.forename: Wenting
authors.initials: W
authors.lastname: Du
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.forename: Hai
authors.initials: H
authors.lastname: Wu
authors.forename: Jingqiang
authors.initials: J
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Yao
authors.initials: Y
authors.lastname: Li
mesh_terms: D020413:3' Untranslated Regions; D000728:Androgens / Q000378:metabolism / Q000494:pharmacology; D017209:Apoptosis / Q000235:genetics; D000071183:Autophagy-Related Proteins; D001483:Base Sequence; D001665:Binding Sites; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D055785:Gene Knockdown Techniques; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011379:Prognosis; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000235:genetics* / Q000401:mortality / Q000628:therapy; D011485:Protein Binding; D011505:Protein-Tyrosine Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D011944:Receptors, Androgen / Q000378:metabolism; D012008:Recurrence; D016896:Treatment Outcome
chemical_list: D020413:3' Untranslated Regions; D000728:Androgens; D000071183:Autophagy-Related Proteins; C548234:MIRN133 microRNA, human; D035683:MicroRNAs; C407704:RB1CC1 protein, human; D011944:Receptors, Androgen; D011505:Protein-Tyrosine Kinases