title: Expression of translationally controlled tumor protein (TCTP) gene of Dirofilaria immitis guided by transcriptomic screening.
abstract: Dirofilaria immitis (heartworm) infections affect domestic dogs, cats, and various wild mammals with increasing incidence in temperate and tropical areas. More sensitive antibody detection methodologies are required to diagnose asymptomatic dirofilariasis with low worm burdens. Applying current transcriptomic technologies would be useful to discover potential diagnostic markers for D. immitis infection. A filarial homologue of the mammalian translationally controlled tumor protein (TCTP) was initially identified by screening the assembled transcriptome of D. immitis (DiTCTP). A BLAST analysis suggested that the DiTCTP gene shared the highest similarity with TCTP from Loa loa at protein level (97%). A histidine-tagged recombinant DiTCTP protein (rDiTCTP) of 40 kDa expressed in Escherichia coli BL21 (DE3) showed immunoreactivity with serum from a dog experimentally infected with heartworms. Localization studies illustrated the ubiquitous presence of rDiTCTP protein in the lateral hypodermal chords, dorsal hypodermal chord, muscle, intestine, and uterus in female adult worms. Further studies on D. immitis-derived TCTP are warranted to assess whether this filarial protein could be used for a diagnostic purpose.
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Fu
authors.affiliation: The Sichuan Key Laboratory for Conservation Biology on Endangered Wildlife, Developing Toward a State Key Laboratory for China, Chengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan 610081, China.
authors.forename: Jingchao
authors.initials: J
authors.lastname: Lan
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Xuhang
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Deying
authors.initials: D
authors.lastname: Yang
authors.affiliation: The Sichuan Key Laboratory for Conservation Biology on Endangered Wildlife, Developing Toward a State Key Laboratory for China, Chengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan 610081, China.
authors.forename: Zhihe
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Huaming
authors.initials: H
authors.lastname: Nie
authors.affiliation: The Sichuan Key Laboratory for Conservation Biology on Endangered Wildlife, Developing Toward a State Key Laboratory for China, Chengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan 610081, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Hou
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Runhui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Wanpeng
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Yan
authors.affiliation: The Sichuan Key Laboratory for Conservation Biology on Endangered Wildlife, Developing Toward a State Key Laboratory for China, Chengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan 610081, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Yang
authors.affiliation: The Sichuan Key Laboratory for Conservation Biology on Endangered Wildlife, Developing Toward a State Key Laboratory for China, Chengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan 610081, China.
authors.forename: Chengdong
authors.initials: C
authors.lastname: Wang
authors.affiliation: The Sichuan Key Laboratory for Conservation Biology on Endangered Wildlife, Developing Toward a State Key Laboratory for China, Chengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan 610081, China.
authors.forename: Li
authors.initials: L
authors.lastname: Luo
authors.affiliation: The Sichuan Key Laboratory for Conservation Biology on Endangered Wildlife, Developing Toward a State Key Laboratory for China, Chengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan 610081, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Gu
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Shuxian
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Xuerong
authors.initials: X
authors.lastname: Peng
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Guangyou
authors.initials: G
authors.lastname: Yang
mesh_terms: D000825:Animal Structures / Q000737:chemistry; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification*; D014408:Biomarkers, Tumor / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification*; D003001:Cloning, Molecular; D004183:Dirofilaria immitis / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D004195:Disease Models, Animal; D004285:Dogs; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D000090324:Tumor Protein, Translationally-Controlled 1
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D014408:Biomarkers, Tumor; D011993:Recombinant Fusion Proteins; D000090324:Tumor Protein, Translationally-Controlled 1
keywords: Dirofilaria immitis; dirofilariasis; heartworm; transcriptome; translationally controlled tumor protein