title: New molecular engineering approaches for crystallographic studies of large RNAs.
abstract: Crystallization of RNAs with complex three-dimensional architectures remains a formidable experimental challenge. We review a number of successful heuristics involving engineering of the target RNAs to facilitate crystal contact formation, such as those that enabled the crystallization and structure determination of the cognate tRNA complexes of RNase P holoenzyme and the Stem I domain of the T-box riboswitch. Recently, RNA-targeted antibody Fab fragments and Kink-turn binding proteins have joined the ranks of successful chaperones for RNA crystallization. Lastly, we review the use of structured RNAs to facilitate crystallization of RNA-binding proteins and other RNAs.
authors.affiliation: National Heart, Lung and Blood Institute, 50 South Drive, MSC 8012, Bethesda, MD 20892-8012, USA.
authors.forename: Jinwei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: National Heart, Lung and Blood Institute, 50 South Drive, MSC 8012, Bethesda, MD 20892-8012, USA. Electronic address: adrian.ferre@nih.gov.
authors.forename: Adrian R
authors.initials: AR
authors.lastname: Ferré-D'Amaré
mesh_terms: D003461:Crystallography / Q000379:methods*; D005818:Genetic Engineering / Q000379:methods*; D006801:Humans; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D012313:RNA / Q000737:chemistry* / Q000235:genetics*; D012343:RNA, Transfer / Q000737:chemistry / Q000235:genetics
chemical_list: D011506:Proteins; D012313:RNA; D012343:RNA, Transfer