title: [Characteristics of enterohemorrhagic Escherichia coli O145:H28 isolated from a patient with hemolytic-uremic syndrome].
abstract: OBJECTIVE
  Determine etiologic significance of clinical strains of E. coli O145:H28 isolated from feces of a patient with hemolytic-uremic syndrome (HUS).
  
  
  METHODS
  20 E. coli strains isolated from feces of a patient with HUS that developed after an acute intestine infection were studied. Antigenic structure of strains was determined by sequencing of rib and fliC genes; presence of virulence genes (pap, aaf, sfa, afa, eaeA, bfpA, ial, hly, cnf, stx1, stx2, LT, ST and aer)--in PCR; ESBL production --by double disk method, ESBL genes--in PCR.
  
  
  RESULTS
  The strains contained rfb gene coding O145, fliC gene coding H7. Genes coding synthesis of stx2-toxin and intimin (eaeA) were detected. The strains were resistant to beta-lactams due to production of CTX-M class ESBL.
  
  
  CONCLUSIONS
  A causative agent E. coli O145:H28 was isolated from a patient with HUS that produces stx2-toxin and CTX-M class ESBL and has not been previously registered in Russian Federation.
authors.forename: L A
authors.initials: LA
authors.lastname: Kaftyreva
authors.forename: M A
authors.initials: MA
authors.lastname: Makarova
authors.forename: T A
authors.initials: TA
authors.lastname: Konovalova
authors.forename: Z N
authors.initials: ZN
authors.lastname: Matveeva
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D002648:Child; D054324:Enterohemorrhagic Escherichia coli / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000235:genetics / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics; D005408:Flagellin; D006463:Hemolytic-Uremic Syndrome / Q000235:genetics / Q000382:microbiology; D006801:Humans; D008297:Male; D012426:Russia; D017422:Sequence Analysis, DNA; D037521:Virulence Factors / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C516095:FliC protein, E coli; D037521:Virulence Factors; C079397:rfb protein, Bacteria; D005408:Flagellin