title: Cell-penetrating peptide (CPP)-conjugated proteins is an efficient tool for manipulation of human mesenchymal stromal cells.
abstract: Delivery of proteins has been regarded as the safest and most useful application in therapeutic application of stem cells, because proteins can regulate gene expression transiently without any genomic alteration. However, it is difficult to accurately measure efficiency or quantity of intracellular protein uptake. Here, we performed a comparison study of cell-penetrating peptide (CPP)-conjugated protein delivery system using seven arginine and Streptolysin O (SLO)-mediated system. To compare CPP- and SLO-mediated protein delivery systems, we used GFP and ESRRB protein, which is known to regulate pluripotency-related genes, for delivery into human bone marrow stromal cells (hBMSCs) and human testicular stromal cells (hTSCs). We found that CPP-conjugated protein delivery was more efficient, lower cytotoxicity, and higher biological activity than SLO-mediated protein delivery system. These results suggest that delivery of CPP-conjugated proteins is an efficient tool for introducing biologically active proteins into cells and may have important implications in clinical cell-based therapy.
authors.affiliation: 1] Department of Biomedical Science, College of Life Science, CHA University, Seoul, Korea [2].
authors.forename: Junghyun
authors.initials: J
authors.lastname: Jo
authors.affiliation: 1] Department of Biomedical Science, College of Life Science, CHA University, Seoul, Korea [2].
authors.forename: Soomin
authors.initials: S
authors.lastname: Hong
authors.affiliation: 1] Fertility Center, CHA Gangnam Medical Center, College of Medicine, CHA University, Seoul, Korea [2] Department of Biotechnology, Seoul Women's University, Seoul, Korea.
authors.forename: Won Yun
authors.initials: WY
authors.lastname: Choi
authors.affiliation: 1] Department of Biomedical Science, College of Life Science, CHA University, Seoul, Korea [2] Fertility Center, CHA Gangnam Medical Center, College of Medicine, CHA University, Seoul, Korea [3] CHA Stem Cell Institute, CHA University, Seoul, Korea.
authors.forename: Dong Ryul
authors.initials: DR
authors.lastname: Lee
mesh_terms: D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D057846:Cell-Penetrating Peptides / Q000737:chemistry / Q000235:genetics; D002478:Cells, Cultured; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000737:chemistry / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*
chemical_list: D057846:Cell-Penetrating Peptides; C490918:ESRRB protein, human; D011960:Receptors, Estrogen; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins