title: Surface plasmon resonance for cell-based clinical diagnosis.
abstract: Non-invasive real-time observations and the evaluation of living cell conditions and functions are increasingly demanded in life sciences. Surface plasmon resonance (SPR) sensors detect the refractive index (RI) changes on the surface of sensor chips in label-free and on a real-time basis. Using SPR sensors, we and other groups have developed techniques to evaluate living cells' reactions in response to stimuli without any labeling in a real-time manner. The SPR imaging (SPRI) system for living cells may visualize single cell reactions and has the potential to expand application of SPR cell sensing for clinical diagnosis, such as multi-array cell diagnostic systems and detection of malignant cells among normal cells in combination with rapid cell isolation techniques.
authors.affiliation: Department of Dermatology, Division of Molecular Medical Science, Graduate School of Biomedical Science, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8551, Japan. yyanase@hiroshima-u.ac.jp.
authors.forename: Yuhki
authors.initials: Y
authors.lastname: Yanase
authors.affiliation: Department of Dermatology, Division of Molecular Medical Science, Graduate School of Biomedical Science, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8551, Japan. hiragunt@hiroshima-u.ac.jp.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Hiragun
authors.affiliation: Department of Dermatology, Division of Molecular Medical Science, Graduate School of Biomedical Science, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8551, Japan. ishiik@hiroshima-u.ac.jp.
authors.forename: Kaori
authors.initials: K
authors.lastname: Ishii
authors.affiliation: Department of Dermatology, Division of Molecular Medical Science, Graduate School of Biomedical Science, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8551, Japan. tomokok@hiroshima-u.ac.jp.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kawaguchi
authors.affiliation: Department of Dermatology, Division of Molecular Medical Science, Graduate School of Biomedical Science, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8551, Japan. yanaset@hiroshima-u.ac.jp.
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Yanase
authors.affiliation: Department of Dermatology, Division of Molecular Medical Science, Graduate School of Biomedical Science, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8551, Japan. mkawai02@hiroshima-u.ac.jp.
authors.forename: Mikio
authors.initials: M
authors.lastname: Kawai
authors.affiliation: Center for Microelectronic systems, Kyushu Institute of Technology, 680-4, Kawazu, Iizuka, Fukuoka, 820-8502, Japan. sakamoto@cms.kyutech.ac.jp.
authors.forename: Kenji
authors.initials: K
authors.lastname: Sakamoto
authors.affiliation: Department of Dermatology, Division of Molecular Medical Science, Graduate School of Biomedical Science, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8551, Japan. ed1h-w1de-road@hiroshima-u.ac.jp.
authors.forename: Michihiro
authors.initials: M
authors.lastname: Hide
mesh_terms: D000818:Animals; D002477:Cells / Q000378:metabolism*; D019937:Diagnostic Techniques and Procedures; D006801:Humans; D055100:Optical Fibers; D012815:Signal Processing, Computer-Assisted; D059010:Single-Cell Analysis; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*