title: Genetic diversity based on SSR analysis of the cultured snakehead fish, Channa argus, (Channidae) in China.
abstract: The snakehead fish Channa argus is an important food fish in China. We identified six microsatellite loci for C. argus. These six microsatellite loci and four other microsatellite markers were used to analyze genetic diversity in four cultured populations of C. argus (SD, JX, HN, and ZJ) and determine their relationships. A total of 154 alleles were detected at the 10 microsatellite loci. The average expected and observed heterozygosities varied from 0.70-0.84 and 0.69-0.83, respectively, and polymorphism information content ranged between 0.66 and 0.82 in the four populations, indicating high genetic diversity. Population JX deviated from mutation-drift equilibrium and may have experienced a recent bottleneck. Analysis of pairwise genetic differentiation revealed FST values that ranged from 0.028 to 0.100, which indicates a moderate level of genetic differentiation. The largest distances were observed between populations HN and SD, whereas the smallest distances were obtained between populations HN and JX. Genetic clustering analysis demonstrated that the ZJ and HN populations probably share the same origin. This information about the genetic diversity within each of the four populations, and their genetic relationships will be useful for future genetic improvement of C. argus through selective breeding.
authors.affiliation: Key Laboratory of Freshwater Aquatic Genetic Resources Certified by Ministry of Agriculture, Shanghai Ocean University, Shanghai, China.
authors.forename: S-R
authors.initials: SR
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Freshwater Aquatic Genetic Resources Certified by Ministry of Agriculture, Shanghai Ocean University, Shanghai, China jlli2009@126.com.
authors.forename: J-L
authors.initials: JL
authors.lastname: Li
authors.affiliation: Institute of Fisheries, Hangzhou Academy of Agriculture Science, Hangzhou, China.
authors.forename: N
authors.initials: N
authors.lastname: Xie
authors.affiliation: Institute of Fisheries, Hangzhou Academy of Agriculture Science, Hangzhou, China.
authors.forename: L-M
authors.initials: LM
authors.lastname: Zhu
authors.affiliation: Department of Biology, East China Normal University, Shanghai, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Molecular Population Genetics Group, Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.
authors.forename: G-H
authors.initials: GH
authors.lastname: Yue
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D017931:DNA Primers; D005399:Fishes / Q000145:classification / Q000235:genetics*; D014644:Genetic Variation; D018895:Microsatellite Repeats
chemical_list: D017931:DNA Primers; D004247:DNA