title: Evaluation of hydrogen production by clostridium strains on beet molasses.
abstract: Clostridium acetobutylicum DSM 792, C. acetobutylicum DSM 1731 and two newly isolated bacteria defined as the members of genus Clostridium - based on the 16S rRNA analysis and biochemical traits - were characterized with regard to their hydrogen production in media containing increasing beet molasses concentrations. The highest hydrogen yield was observed for C. acetobutylicum DSM 792 with a yield of 2.8 mol H2 mol-1 hexose in medium including 60 g L-1 molasses. This bacterium also produced the maximum amount of hydrogen (5908.8 mL L-1) at the same molasses concentration. A slightly lower hydrogen yield was measured for C. acetobutylicum DSM 1731 (2.5 mol H2 mol-1 hexose) when grown on 40 g L-1 molasses. The new isolates Clostridium roseum C and Clostridium saccharoperbutylacetonicum PF produced hydrogen with yields of 2.0 mol H2 mol-1 hexose at 40 and 60 g L-1 molasses and 2.1 mol H2 mol-1 hexose at 40 gL-1 molasses, respectively.
authors.affiliation: Department of Food Engineering, Sakarya University, 54187 Sakarya, Turkey. aysea@sakarya.edu.tr
authors.forename: Ayşe
authors.initials: A
authors.lastname: Avci
authors.affiliation: Department of Biology, Ankara University, 06100 Beşevler, Ankara, Turkey.
authors.forename: Nur Koçberber
authors.initials: NK
authors.lastname: Kiliç
authors.affiliation: Department of Biology, Ankara University, 06100 Beşevler, Ankara, Turkey.
authors.forename: Gönöl
authors.initials: G
authors.lastname: Dönmez
authors.affiliation: Department of Food Engineering, Ankara University, 06110 Ditkapi, Ankara, Turkey.
authors.forename: Sedat
authors.initials: S
authors.lastname: Dönmez
mesh_terms: D027461:Beta vulgaris / Q000382:microbiology*; D056804:Biofuels / Q000382:microbiology*; D003013:Clostridium / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology*; D006859:Hydrogen / Q000302:isolation & purification* / Q000378:metabolism*; D008965:Molasses / Q000382:microbiology*; D013045:Species Specificity
chemical_list: D056804:Biofuels; D006859:Hydrogen