title: OsMOGS is required for N-glycan formation and auxin-mediated root development in rice (Oryza sativa L.).
abstract: N-glycosylation is a major modification of glycoproteins in eukaryotic cells. In Arabidopsis, great progress has been made in functional analysis of N-glycan production, however there are few studies in monocotyledons. Here, we characterized a rice (Oryza sativa L.) osmogs mutant with shortened roots and isolated a gene that coded a putative mannosyl-oligosaccharide glucosidase (OsMOGS), an ortholog of α-glucosidase I in Arabidopsis, which trims the terminal glucosyl residue of the oligosaccharide chain of nascent peptides in the endoplasmic reticulum (ER). OsMOGS is strongly expressed in rapidly cell-dividing tissues and OsMOGS protein is localized in the ER. Mutation of OsMOGS entirely blocked N-glycan maturation and inhibited high-mannose N-glycan formation. The osmogs mutant exhibited severe defects in root cell division and elongation, resulting in a short-root phenotype. In addition, osmogs plants had impaired root hair formation and elongation, and reduced root epidemic cell wall thickness due to decreased cellulose synthesis. Further analysis showed that auxin content and polar transport in osmogs roots were reduced due to incomplete N-glycosylation of the B subfamily of ATP-binding cassette transporter proteins (ABCBs). Our results demonstrate that involvement of OsMOGS in N-glycan formation is required for auxin-mediated root development in rice.
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: SuiKang
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: YanXia
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Key Laboratory of Crop Germplasm Resources of Zhejiang Province, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.
authors.forename: ZhiLan
authors.initials: Z
authors.lastname: Li
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: SaiNa
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Changwon National University, Changwon, Gyeongnam 641-773, Republic of Korea.|Complex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Road, Athens, Georgia 30602-4712, USA.
authors.forename: Jae-Min
authors.initials: JM
authors.lastname: Lim
authors.affiliation: Department of Biochemistry and PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju, 660-701, Republic of Korea.
authors.forename: Kyun Oh
authors.initials: KO
authors.lastname: Lee
authors.affiliation: State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: ChuanYou
authors.initials: C
authors.lastname: Li
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, 359 Tiyuchang Road, Hangzhou, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Qian
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: De An
authors.initials: A
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: YanHua
authors.initials: Y
authors.lastname: Qi
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001692:Biological Transport; D002455:Cell Division; D048429:Cell Size; D002473:Cell Wall / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D006031:Glycosylation; D007210:Indoleacetic Acids / Q000378:metabolism*; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009154:Mutation; D012275:Oryza / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010802:Phylogeny; D019441:Plant Epidermis / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000520:alpha-Glucosidases / Q000145:classification / Q000235:genetics / Q000378:metabolism*
chemical_list: D018528:ATP-Binding Cassette Transporters; D007210:Indoleacetic Acids; D010940:Plant Proteins; D011134:Polysaccharides; C042059:glucosidase I; D000520:alpha-Glucosidases
keywords: N-glycan formation; OsMOGS; auxin; cellulose synthesis; rice (Oryza sativa L.); root development