title: New Arabidopsis advanced intercross recombinant inbred lines reveal female control of nonrandom mating.
abstract: Female control of nonrandom mating has never been genetically established, despite being linked to inbreeding depression and sexual selection. In order to map the loci that control female-mediated nonrandom mating, we constructed a new advanced intercross recombinant inbred line (RIL) population derived from a cross between Arabidopsis (Arabidopsis thaliana) accessions Vancouver (Van-0) and Columbia (Col-0) and mapped quantitative trait loci (QTLs) responsible for nonrandom mating and seed yield traits. We genotyped a population of 490 RILs. A subset of these lines was used to construct an expanded map of 1,061.4 centimorgans with an average interval of 6.7±5.3 centimorgans between markers. QTLs were then mapped for female- and male-mediated nonrandom mating and seed yield traits. To map the genetic loci responsible for female-mediated nonrandom mating and seed yield, we performed mixed pollinations with genetically marked Col-0 pollen and Van-0 pollen on RIL pistils. To map the loci responsible for male-mediated nonrandom mating and seed yield, we performed mixed pollinations with genetically marked Col-0 and RIL pollen on Van-0 pistils. Composite interval mapping of these data identified four QTLs that control female-mediated nonrandom mating and five QTLs that control female-mediated seed yield. We also identified four QTLs that control male-mediated nonrandom mating and three QTLs that control male-mediated seed yield. Epistasis analysis indicates that several of these loci interact. To our knowledge, the results of these experiments represent the first time female-mediated nonrandom mating has been genetically defined.
authors.affiliation: Department of Biology, Rhodes College, Memphis, Tennessee 38112.
authors.forename: Jonathan Nesbit
authors.initials: JN
authors.lastname: Fitz Gerald
authors.forename: Ann Louise
authors.initials: AL
authors.lastname: Carlson
authors.forename: Evadne
authors.initials: E
authors.lastname: Smith
authors.forename: Julin N
authors.initials: JN
authors.lastname: Maloof
authors.forename: Detlef
authors.initials: D
authors.lastname: Weigel
authors.forename: Joanne
authors.initials: J
authors.lastname: Chory
authors.forename: Justin O
authors.initials: JO
authors.lastname: Borevitz
authors.forename: Robert John
authors.initials: RJ
authors.lastname: Swanson
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004843:Epistasis, Genetic; D005819:Genetic Markers; D007178:Inbreeding; D040582:Inheritance Patterns / Q000235:genetics; D010641:Phenotype; D040641:Quantitative Trait Loci / Q000235:genetics; D019655:Quantitative Trait, Heritable; D011995:Recombination, Genetic / Q000235:genetics*; D012098:Reproduction; D012639:Seeds / Q000235:genetics / Q000254:growth & development
chemical_list: D005819:Genetic Markers