title: The evaluation of different treatment protocols for trauma-induced lung injury in rats.
abstract: BACKGROUND
  Lung contusion is an important factor that affects mortality and morbidity of lung injury after blunt chest trauma (BCT). The present study aims to evaluate the effectiveness of different treatment regimens on BCT-induced lung injury.
  
  
  METHODS
  A total of 35 Sprague Dawley rats were divided into five experimental groups (n=7): sham, control; BCT; BCT + MP, BCT group treated with methylprednisolone (MP; 30 mg/kg on first day and 3 mg/kg/d on the following days); BCT + Q, BCT group treated with quercetin (Q; 50 mg/kg/d for seven days); and BCT + MP + Q, BCT group treated with the same doses of MP and Q. Serum Clara Cell Protein-16 (CC-16), thiobarbituric acid reactive substances (TBARS), and superoxide dismutase (SOD) levels were analyzed to determine histopathological changes in the lung tissues.
  
  
  RESULTS
  Elevated serum CC-16 and TBARS levels and reduced serum SOD levels were found in the BCT group compared to the Sham group. There was a significant change in the serum CC-16 levels in the BCT + MP group compared to the Sham group. Serum TBARS levels were significantly lower in the BCT + MP and BCT + Q group compared to the BCT group. The combined therapy regimen yielded significantly decreased CC-16 levels and increased serum SOD levels compared to the individual treatment groups. Serum TBARS levels did not significantly differ between the BCT + MP + Q group and the other treatment groups. Compared to the BCT + MP + Q group, the BCT + MP group showed significantly lower alveolar edema (AED) and alveolar exudate (AEX) scores, while the BCT + Q group showed significantly lower peribronchial inflammatory cell infiltration (PICI) and AED scores.
  
  
  CONCLUSIONS
  The combined usage of quercetin and low dose MP treatment after initial high dose MP at the early stage of lung injury after BCT is more effective.
authors.affiliation: 1 Faculty of Medicine, Department of Emergency Medicine, 2 Faculty of Medicine, Department of Chest Diseases, 3 Faculty of Medicine, Department of Medical Biochemistry, 4 Faculty of Veterinary Medicine, Department of Pathology, 5 Department of Industrial Engineering, Ondokuz Mayıs University, Samsun, Turkey.
authors.forename: Hızır Ufuk
authors.initials: HU
authors.lastname: Akdemir
authors.affiliation: 1 Faculty of Medicine, Department of Emergency Medicine, 2 Faculty of Medicine, Department of Chest Diseases, 3 Faculty of Medicine, Department of Medical Biochemistry, 4 Faculty of Veterinary Medicine, Department of Pathology, 5 Department of Industrial Engineering, Ondokuz Mayıs University, Samsun, Turkey.
authors.forename: Aygül
authors.initials: A
authors.lastname: Güzel
authors.affiliation: 1 Faculty of Medicine, Department of Emergency Medicine, 2 Faculty of Medicine, Department of Chest Diseases, 3 Faculty of Medicine, Department of Medical Biochemistry, 4 Faculty of Veterinary Medicine, Department of Pathology, 5 Department of Industrial Engineering, Ondokuz Mayıs University, Samsun, Turkey.
authors.forename: Celal
authors.initials: C
authors.lastname: Katı
authors.affiliation: 1 Faculty of Medicine, Department of Emergency Medicine, 2 Faculty of Medicine, Department of Chest Diseases, 3 Faculty of Medicine, Department of Medical Biochemistry, 4 Faculty of Veterinary Medicine, Department of Pathology, 5 Department of Industrial Engineering, Ondokuz Mayıs University, Samsun, Turkey.
authors.forename: Latif
authors.initials: L
authors.lastname: Duran
authors.affiliation: 1 Faculty of Medicine, Department of Emergency Medicine, 2 Faculty of Medicine, Department of Chest Diseases, 3 Faculty of Medicine, Department of Medical Biochemistry, 4 Faculty of Veterinary Medicine, Department of Pathology, 5 Department of Industrial Engineering, Ondokuz Mayıs University, Samsun, Turkey.
authors.forename: Hasan
authors.initials: H
authors.lastname: Alaçam
authors.affiliation: 1 Faculty of Medicine, Department of Emergency Medicine, 2 Faculty of Medicine, Department of Chest Diseases, 3 Faculty of Medicine, Department of Medical Biochemistry, 4 Faculty of Veterinary Medicine, Department of Pathology, 5 Department of Industrial Engineering, Ondokuz Mayıs University, Samsun, Turkey.
authors.forename: Ayhan
authors.initials: A
authors.lastname: Gacar
authors.affiliation: 1 Faculty of Medicine, Department of Emergency Medicine, 2 Faculty of Medicine, Department of Chest Diseases, 3 Faculty of Medicine, Department of Medical Biochemistry, 4 Faculty of Veterinary Medicine, Department of Pathology, 5 Department of Industrial Engineering, Ondokuz Mayıs University, Samsun, Turkey.
authors.forename: Tolga
authors.initials: T
authors.lastname: Güvenç
authors.affiliation: 1 Faculty of Medicine, Department of Emergency Medicine, 2 Faculty of Medicine, Department of Chest Diseases, 3 Faculty of Medicine, Department of Medical Biochemistry, 4 Faculty of Veterinary Medicine, Department of Pathology, 5 Department of Industrial Engineering, Ondokuz Mayıs University, Samsun, Turkey.
authors.forename: Naci
authors.initials: N
authors.lastname: Murat
authors.affiliation: 1 Faculty of Medicine, Department of Emergency Medicine, 2 Faculty of Medicine, Department of Chest Diseases, 3 Faculty of Medicine, Department of Medical Biochemistry, 4 Faculty of Veterinary Medicine, Department of Pathology, 5 Department of Industrial Engineering, Ondokuz Mayıs University, Samsun, Turkey.
authors.forename: Bülent
authors.initials: B
authors.lastname: Sişman
keywords: Blunt; chest; quercetin; steroid; trauma