title: Effect of 20-hydroxyeicosatetraenoic acid on biological behavior of human villous trophoblasts and uterine vascular smooth muscle cells.
abstract: During pregnancy, disorders in uterine spiral artery remodeling (USAR) cause preeclampsia and other diseases. The aim of this study was to investigate the effect of 20-hydroxyeicosatetraenoic acid (20-HETE) on the biological behavior of human villous trophoblasts (HVTs) and human uterine vascular smooth muscle cells (HUVSMCs), and explore the role of 20-HETE in USAR. 20-HETE and its inhibitor HET0016 were used to study migration, invasion and apoptosis in the HVT and HVT-HUVSMC models. 20-HETE inhibited the migration and invasion of HVTs, and inhibited apoptosis in HUVSMCs and HUVSMCs co-cultured with HVTs. 20-HETE had thus obvious effects on the biological behavior of HVTs and HUVSMCs. These effects may cause USAR disorders and vascular dysfunction, leading to preeclampsia.
authors.affiliation: Department of Clinical Laboratory, The Affiliated Hospital of Ningbo University, Ningbo, Zhejiang 315000, P.R. China.
authors.forename: Shuaijun
authors.initials: S
authors.lastname: Lu
authors.affiliation: Department of Gynaecology and Obstetrics, The Affiliated Hospital of Ningbo University, Ningbo, Zhejiang 315000, P.R. China.
authors.forename: Changling
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Department of Clinical Laboratory, Baoshan Branch of Shanghai First People's Hospital, Shanghai 200940, P.R. China.
authors.forename: Anxiong
authors.initials: A
authors.lastname: Long
authors.affiliation: Department of Clinical Laboratory, Baoshan Branch of Shanghai First People's Hospital, Shanghai 200940, P.R. China.
authors.forename: Longyi
authors.initials: L
authors.lastname: Tan
authors.affiliation: Department of Clinical Laboratory, Baoshan Branch of Shanghai First People's Hospital, Shanghai 200940, P.R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory, Baoshan Branch of Shanghai First People's Hospital, Shanghai 200940, P.R. China.
authors.forename: Yuli
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000494:pharmacology*; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000378:metabolism; D011247:Pregnancy; D014327:Trophoblasts / Q000187:drug effects* / Q000378:metabolism; D014599:Uterus / Q000187:drug effects* / Q000378:metabolism
chemical_list: D006893:Hydroxyeicosatetraenoic Acids; C055987:20-hydroxy-5,8,11,14-eicosatetraenoic acid; D020778:Matrix Metalloproteinase 2