title: Tumour necrosis factor-alpha, interleukin-10, interferon-gamma and vitamin D receptor gene polymorphisms in patients with chronic hepatitis delta.
abstract: No data exist to assess certain polymorphisms that have a potential effect on the immune response in patients with chronic hepatitis delta (CHD). The aim of this study was to investigate polymorphisms in 6 polymorphic sites: IL-10 -1082 (rs1800896), IL-10 -627 (rs1800872), IFN-γ +874 (rs62559044), TNF-α -308 (rs1800629), vitamin D receptor (VDR) FokI (rs2228570) and VDR TaqI (rs731236). The genotypes of 67 patients with CHD and 119 patients with chronic hepatitis B (CHB) were compared. In addition, 56 individuals with resolved hepatitis B virus (HBV) infection were used as a control group for patients with CHB. Polymorphisms in TNF-α, IL-10, and VDR genes were analysed using polymerase chain reaction/restriction fragment length polymorphism methods. The IFN-γ gene polymorphism was detected by allele-specific polymerase chain reaction (PCR). Patients with CDH were more likely to have advanced liver disease compared with patients with CHB (P < 0.0001). IL-10 -1082 and VDR TaqI polymorphisms showed significant differences between patients with CHD and CHB. The high secretory IL-10 -1082 genotype GG was less frequent in CHD compared with patients with CHB and resolved HBV (17.7%, 37.4% and 47.1%, respectively (P < 0.05 for CHD vs CHB and resolved HBV). The frequency of the high secretory VDR TaqI TT genotype was 86.6% in patients with CHD, 62.7% in patients with CHB and 62.5% in resolved HBV individuals (CHD vs CHB: P < 0.05). None of the polymorphisms analysed had an effect on HBV persistence. IL-10 -1082 and VDR TaqI polymorphisms may contribute to the more severe liver disease associated with CHD compared with CHB.
authors.affiliation: Hepatology Institute, Ankara University, Ankara, Turkey.
authors.forename: S C
authors.initials: SC
authors.lastname: Karatayli
authors.forename: Z E
authors.initials: ZE
authors.lastname: Ulger
authors.forename: A A
authors.initials: AA
authors.lastname: Ergul
authors.forename: O
authors.initials: O
authors.lastname: Keskin
authors.forename: E
authors.initials: E
authors.lastname: Karatayli
authors.forename: R
authors.initials: R
authors.lastname: Albayrak
authors.forename: M
authors.initials: M
authors.lastname: Ozkan
authors.forename: R
authors.initials: R
authors.lastname: Idilman
authors.forename: K
authors.initials: K
authors.lastname: Yalcin
authors.forename: H
authors.initials: H
authors.lastname: Bozkaya
authors.forename: O
authors.initials: O
authors.lastname: Uzunalimoğlu
authors.forename: C
authors.initials: C
authors.lastname: Yurdaydin
authors.forename: A M
authors.initials: AM
authors.lastname: Bozdayi
mesh_terms: D000328:Adult; D000483:Alleles; D015331:Cohort Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006515:Hepatitis B virus / Q000502:physiology; D019694:Hepatitis B, Chronic / Q000235:genetics / Q000821:virology; D019701:Hepatitis D, Chronic / Q000235:genetics* / Q000821:virology; D003698:Hepatitis Delta Virus / Q000502:physiology*; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D016753:Interleukin-10 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D018167:Receptors, Calcitriol / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D014421:Turkey; D019562:Viral Load
chemical_list: D018167:Receptors, Calcitriol; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma
keywords: chronic hepatitis B; chronic hepatitis delta; cytokine genes; polymorphism; vitamin D receptor gene