title: Application of ZnO quantum dots dotted carbon nanotube for sensitive electrochemiluminescence immunoassay based on simply electrochemical reduced Pt/Au alloy and a disposable device.
abstract: We report on a disposable microdevice suitable for sandwich-type electrochemiluminescence (ECL) detection of prostate specific antigen (PSA). The method is making use of ZnO quantum dots dotted carbon nanotube (ZnO@CNT) and simply electrochemical reduced Pt/Au alloy. The latter was selected as immunosensing probe to modify screen-printed carbon electrode, due to its excellent electrical property. For further ultrasensitive, low-potential and stable ECL detection, ZnO@CNT composite was first synthesized using a facile solvothermal method, and employed as signal amplification label. In this work, two working electrodes in one device were used for one determination to obtain more exact results based on screen-print technique. Taking advantage of dual-amplification effects of the Pt/Au and ZnO@CNT, this immunosensor could detect the PSA quantitatively, in the range of 0.001-500 ng mL(-1), with a low detection limit of 0.61 pg mL(-1). The resulting versatile immunosensor possesses high sensitivity, satisfactory reproducibility and regeneration. This simple and specific strategy has vast potential to be used in other biological assays.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, PR China.
authors.forename: Wenping
authors.initials: W
authors.lastname: Deng
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Shandong Provincial Key Laboratory of Preparation and Measurement of Building Materials, University of Jinan, Jinan, 250022, PR China.
authors.forename: Shenguang
authors.initials: S
authors.lastname: Ge
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, PR China. Electronic address: ujn.yujh@gmail.com.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yu
authors.affiliation: Cancer Research Center, Shandong Tumor Hospital, Jinan, 250012, PR China. Electronic address: sxr@vip.163.com.
authors.forename: Xianrang
authors.initials: X
authors.lastname: Song
mesh_terms: D000497:Alloys / Q000737:chemistry*; D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D055664:Electrochemical Techniques; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008163:Luminescent Measurements; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010084:Oxidation-Reduction; D010984:Platinum / Q000737:chemistry; D017430:Prostate-Specific Antigen / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification; D045663:Quantum Dots / Q000737:chemistry*; D052616:Solid Phase Extraction; D015034:Zinc Oxide / Q000737:chemistry
chemical_list: D000497:Alloys; D055910:Antibodies, Immobilized; D037742:Nanotubes, Carbon; D010984:Platinum; D006046:Gold; D017430:Prostate-Specific Antigen; D015034:Zinc Oxide
keywords: Electrochemiluminescence; Immunosensor; Pt/Au alloy; Screen-printed electrode; ZnO quantum dots dotted carbon nanotube