title: Chromatographic determination of low-molecular mass unsaturated aliphatic aldehydes with peroxyoxalate chemiluminescence detection after fluorescence labeling with 4-(N,N-dimethylaminosulfonyl)-7-hydrazino-2,1,3-benzoxadiazole.
abstract: A highly sensitive, selective and reproducible chromatographic method is described for determination of low-molecular mass unsaturated aliphatic aldehydes in human serum. The method combines fluorescent labeling using 4-(N,N-Dimethylaminosulfonyl)-7-hydrazino-2,1,3-benzoxadiazole with peroxyoxalate chemiluminescence. The derivatives were separated on a reversed-phase column C8 isocratically using a mixture of acetonitrile and 90mM imidazole-HNO3 buffer (pH 6.4, 1:1, % v/v). The calibration ranges were: 20-420nM for methylglyoxal, 16-320nM for acrolein, 15-360nM for crotonaldehyde and 20-320nM for trans-2-hexenal. The detection limits were ranged from 4.4 to 6.5nM (88-130fmol/injection), the recovery results were within the range of 87.4-103.8% and the intra and inter-day precision results were lower than 5.5%. The proposed validated method has been successfully applied to healthy, diabetic and rheumatic arthritis patients' sera with simple pretreatment method. In conclusion, this new method is suitable for routine analysis of large numbers of clinical samples for assessment of the oxidative stress state in patients.
authors.affiliation: Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt; Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
authors.forename: Marwa Fathy Bakr
authors.initials: MF
authors.lastname: Ali
authors.affiliation: Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Kishikawa
authors.affiliation: Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
authors.forename: Kaname
authors.initials: K
authors.lastname: Ohyama
authors.affiliation: Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt.
authors.forename: Horria Abdel-Mageed
authors.initials: HA
authors.lastname: Mohamed
authors.affiliation: Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt.
authors.forename: Hanaa Mohamed
authors.initials: HM
authors.lastname: Abdel-Wadood
authors.affiliation: Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt.
authors.forename: Ashraf Mohamed
authors.initials: AM
authors.lastname: Mahmoud
authors.affiliation: Sasebo Chu Hospital, 5 Yamato-machi, Sasebo 857-1165, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Imazato
authors.affiliation: Sasebo Chu Hospital, 5 Yamato-machi, Sasebo 857-1165, Japan.
authors.forename: Yukitaka
authors.initials: Y
authors.lastname: Ueki
authors.affiliation: Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Wada
authors.affiliation: Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan. Electronic address: n-kuro@nagasaki-u.ac.jp.
authors.forename: Naotaka
authors.initials: N
authors.lastname: Kuroda
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000447:Aldehydes / Q000097:blood*; D001172:Arthritis, Rheumatoid; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003920:Diabetes Mellitus; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D010069:Oxadiazoles / Q000737:chemistry*; D018384:Oxidative Stress; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D013449:Sulfonamides / Q000737:chemistry*
chemical_list: D000447:Aldehydes; D005456:Fluorescent Dyes; D010069:Oxadiazoles; D013449:Sulfonamides; C080612:4-(N,N-dimethylaminosulfonyl)-7-hydrazino-2,1,3-benzoxadiazole; C051750:2-hexenal; C012796:2-butenal
keywords: Fluorescence labeling; Lipid peroxidation; Oxidative stress; Peroxyoxalate chemiluminescence (PO-CL); Serum analysis