title: The effect of antibacterial agents on fissure sealant microleakage: a 6-month in vitro study.
abstract: OBJECTIVE
  To evaluate microleakage from an antibacterial adhesive and bonding agent added to the ssure sealant on intact enamel.
  
  
  METHODS
  One hundred twenty sound third mandibular molars were randomly divided into 6 groups of 20 teeth each. Occlusal pits and ssures were sealed with un lled sealant material (Clinpro, 3M) after pretreatment with 1. phosphoric acid etching (control); 2. acid etching + Adper Single Bond 2 (SB, 3M); 3. chlorhexidine digluconate (CHX, Ultradent) + acid etching; 4. CHX + acid etching + SB; 5. acid etching + Clear l Protect Bond (CPB, Kuraray) 6. CPB alone. After 6-month water storage and thermocycling, the specimens were placed in 0.5% fuschin, sectioned and evaluated under a digital microscope. The data were compared between groups with Kruskal-Wallis and Mann-Whitney tests.
  
  
  RESULTS
  Differences between groups were statistically signi cant at P < 0.05. Acid etching alone and with SB showed the lowest microleakage, followed by acid etching + CPB. Chlorhexidine with and without bonding agent showed the greatest microleakage.
  
  
  CONCLUSIONS
  Conventional acid etching alone or with a one-bottle adhesive were the two most effective methods of reducing microleakage from ssures. Acid etching together with a self-etching adhesive showed better results than self-etching alone. Appling CHX increased microleakage in sealed teeth.
authors.forename: Mahtab
authors.initials: M
authors.lastname: Memarpour
authors.forename: Fereshteh
authors.initials: F
authors.lastname: Shafiei
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D000891:Anti-Infective Agents, Local / Q000737:chemistry*; D002710:Chlorhexidine / Q000031:analogs & derivatives / Q000737:chemistry; D004396:Coloring Agents; D003188:Composite Resins / Q000737:chemistry; D003738:Dental Cements / Q000737:chemistry; D003743:Dental Enamel / Q000648:ultrastructure; D003763:Dental Leakage / Q000145:classification*; D017220:Dentin-Bonding Agents / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D010756:Phosphoric Acids / Q000737:chemistry; D010895:Pit and Fissure Sealants / Q000737:chemistry*; D012394:Rosaniline Dyes; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: C554641:Adper single bond 2; D000891:Anti-Infective Agents, Local; C503075:Clearfil Protect Bond; C500418:Clinpro Sealant; D004396:Coloring Agents; D003188:Composite Resins; D003738:Dental Cements; D017220:Dentin-Bonding Agents; D010756:Phosphoric Acids; D010895:Pit and Fissure Sealants; D012394:Rosaniline Dyes; D014867:Water; C025485:basic fuchsin; C030242:phosphoric acid; C010882:chlorhexidine gluconate; D002710:Chlorhexidine