title: Biomaterial properties of cholecyst-derived scaffold recovered by a non-detergent/enzymatic method.
abstract: Isolation procedures for the recovery of extracellular matrices (ECMs) from animal organs/tissues that are useful in regenerative medicine involve multiple sequential steps/stages including collection of the source organ at slaughter, their transportation to laboratory, decellularization, decontamination, stabilization, and sterilization. Most of these steps require extensive use of chemicals/reagents/enzymes which may also adversely affect the quality of the scaffold. With an effort to minimize the use of chemicals/reagents/enzymes, while extracting biomaterial-grade ECM from porcine cholecyst (gall bladder), we performed preisolation ex situ incubation of the organ in a stabilizing agent that also caused in situ crosslinking of tissue-components and delaminated the collagen-rich ECM from the tissue-layer beneath the mucosa. The physical, chemical, and biological properties of the isolated scaffolds were similar to that of a commercially available porcine small intestinal submucosa. The cholecyst-derived scaffold not only satisfied preclinical safety-test procedures such as cytotoxicity, local response, and endotoxin load but also showed the potential to promote healing of full-thickness skin wound in a rabbit model. The procedure was also suitable for isolating scaffolds from other hollow organs such as jejunum and urinary bladder. It was concluded that enzyme/detergent treatment may be an avoidable step while isolating biomaterial-grade scaffolds from hollow organs.
authors.affiliation: Division of Experimental Pathology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, 695012, Kerala, India.
authors.forename: Thapasimuthu V
authors.initials: TV
authors.lastname: Anilkumar
authors.forename: Vadavanath P
authors.initials: VP
authors.lastname: Vineetha
authors.forename: Deepa
authors.initials: D
authors.lastname: Revi
authors.forename: Jaseer
authors.initials: J
authors.lastname: Muhamed
authors.forename: Akhila
authors.initials: A
authors.lastname: Rajan
mesh_terms: D000818:Animals; D001705:Bioprosthesis; D002460:Cell Line; D005109:Extracellular Matrix / Q000737:chemistry*; D005704:Gallbladder / Q000737:chemistry*; D008422:Materials Testing; D011817:Rabbits; D054457:Tissue Scaffolds / Q000737:chemistry*
keywords: acellular tissue-derived scaffold; cholecyst-derived scaffold; decellularized scaffold; extracellular matrix; wound healing