title: Gene expression profile of oxidative stress in the lung of inbred mice after intestinal ischemia/reperfusion injury.
abstract: OBJECTIVE
  To determine the gene expression profile associated with oxidative stress and antioxidant defense in the lung tissue of mice subjected to intestinal ischemia and reperfusion.
  
  
  METHODS
  Twelve male, inbred mice (C57BL/6) were randomly assigned to one of two groups. The control group (CG) underwent anesthesia and laparotomy and was observed for 120 minutes; the ischemia/reperfusion group (IRG) was subjected to anesthesia, laparotomy, and ischemia of the small intestine for 60 minutes and to 60 minutes of reperfusion. A pool of six mice from each group was subjected to a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to analyze the oxidative stress and antioxidant defense genes. All genes that were up-regulated or down-regulated greater than three-fold, based on the algorithm [2^(ΔΔCt)], were considered to be biologically meaningful.
  
  
  RESULTS
  Out of a total of 84 genes in the lung that are related to oxidative stress, 67 (79.7%) were up-regulated and 17 (20.2%) were down-regulated. Only two genes (2.3%), Lpo (lactoperoxidase) (+3.51) and Gpx4 (glutathione peroxidase) (+4.10), were expressed above the three-fold threshold, while none of the down-regulated genes were expressed outside of this threshold.
  
  
  CONCLUSIONS
  The intestinal ischemia/reperfusion injury promoted a gene expression profile consisting of the positive expression of oxidative genes in a remote organ. This suggests that activate signaling pathways are implicated in both cell survival and the maintenance of genome integrity in the lung.
authors.affiliation: Federal University of Grande Dourados, Medical School, DouradosMS, Brazil, Fellow Master degree in Surgery, Postgraduate Program in Interdisciplinary Surgical Sciences, Sao Paulo Federal University (UNIFESP), Sao Paulo-SP. Associate Professor, Medical School, Federal University of Grande Dourados (UFGD), Dourados-MS, Brazil. Intellectual and scientific content of the study, design the protocol, technical procedures, acquisition and interpretation of data, statistical analysis, manuscript writing.
authors.forename: Adauto Tsutomu
authors.initials: AT
authors.lastname: Ikejiri
authors.affiliation: UFGD, Medical School, DouradosMS, Brazil, Fellow Master degree in Surgery, Postgraduate Program in Interdisciplinary Surgical Sciences, UNIFESP, Sao Paulo-SP. Associate Professor, Medical School, UFGD, Dourados-MS, Brazil. Technical procedures, acquisition and interpretation of data, statistical analysis, manuscript writing.
authors.forename: Frederico
authors.initials: F
authors.lastname: Somaio Neto
authors.affiliation: UFGD, Medical School, DouradosMS, Brazil, Fellow PhD degree in Surgery, Postgraduate Program in Interdisciplinary Surgical Sciences, UNIFESP, Sao Paulo-SP. Associate Professor, Medical School, UFGD, Dourados-MS, Brazil. Technical procedures, acquisition and interpretation of data, statistical analysis, manuscript writing.
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Chaves
authors.affiliation: UFGD, Medical School, DouradosMS, Brazil, IVPhD, Associate Professor, Medical School, UFGD, Dourados-MS, Brazil. Technical procedures, acquisition and interpretation of data, statistical analysis, manuscript writing.
authors.forename: Paulo Roberto
authors.initials: PR
authors.lastname: Bertoletto
authors.affiliation: Federal University of Mato Grosso do Sul, Surgery Department, Campo GrandeMS, Brazil, VFellow PhD degree in Surgery, Postgraduate Program in Interdisciplinary Surgical Sciences, UNIFESP, Sao Paulo-SP. Affiliate Professor, Surgery Department, Federal University of Mato Grosso do Sul (UFMS), Campo Grande-MS, Brazil. Technical procedures, acquisition and interpretation of data, statistical analysis, manuscript writing.
authors.forename: Roberto
authors.initials: R
authors.lastname: Teruya
authors.affiliation: Universitary Center Sao Camilo, Sao PauloSP, Brazil, VIGraduate student, Universitary Center Sao Camilo, Sao Paulo-SP, Brazil. Technical procedures, acquisition and interpretation of data.
authors.forename: Eduardo Rodrigues
authors.initials: ER
authors.lastname: Bertoletto
authors.affiliation: UNIFESP, Surgery Department, Sao PauloSP, Brazil, PhD, Associate Professor, Surgical Techniques and Experimental Surgery Division, Surgery Department, UNIFESP, Sao Paulo-SP, Brazil. Manuscript writing, critical revision.
authors.forename: Murched Omar
authors.initials: MO
authors.lastname: Taha
authors.affiliation: UNIFESP, Surgery Department, Sao PauloSP, Brazil, PhD, Associate Professor, Surgical Techniques and Experimental Surgery Division, Surgery Department, UNIFESP, Sao Paulo-SP, Brazil. Intellectual and scientific content of the study, design the protocol, technical procedures, interpretation of data, manuscript writing, critical revision.
authors.forename: Djalma José
authors.initials: DJ
authors.lastname: Fagundes
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000235:genetics; D015870:Gene Expression / Q000235:genetics*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000098:blood supply*; D007511:Ischemia / Q000235:genetics / Q000378:metabolism; D008168:Lung / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000235:genetics*; D011897:Random Allocation; D015427:Reperfusion Injury / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D059467:Transcriptome / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics
chemical_list: D000975:Antioxidants