title: Quantification of deaminase activity-dependent and -independent restriction of HIV-1 replication mediated by APOBEC3F and APOBEC3G through experimental-mathematical investigation.
abstract: APOBEC3F and APOBEC3G cytidine deaminases potently inhibit human immunodeficiency virus type 1 (HIV-1) replication by enzymatically inserting G-to-A mutations in viral DNA and/or impairing viral reverse transcription independently of their deaminase activity. Through experimental and mathematical investigation, here we quantitatively demonstrate that 99.3% of the antiviral effect of APOBEC3G is dependent on its deaminase activity, whereas 30.2% of the antiviral effect of APOBEC3F is attributed to deaminase-independent ability. This is the first report quantitatively elucidating how APOBEC3F and APOBEC3G differ in their anti-HIV-1 modes.
authors.affiliation: Laboratory of Viral Pathogenesis, Institute for Virus Research, Kyoto University, Kyoto, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Koizumi
authors.forename: Junko S
authors.initials: JS
authors.lastname: Takeuchi
authors.forename: Naoko
authors.initials: N
authors.lastname: Misawa
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Kimura
authors.forename: Satoru
authors.initials: S
authors.lastname: Morita
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Aihara
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Koyanagi
authors.forename: Shingo
authors.initials: S
authors.lastname: Iwami
authors.forename: Kei
authors.initials: K
authors.lastname: Sato
mesh_terms: D000071480:APOBEC-3G Deaminase; D002460:Cell Line; D003564:Cytidine Deaminase / Q000378:metabolism*; D043525:Cytosine Deaminase / Q000378:metabolism*; D015497:HIV-1 / Q000276:immunology* / Q000502:physiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D008962:Models, Theoretical; D014779:Virus Replication
chemical_list: C492906:APOBEC3F protein, human; D043525:Cytosine Deaminase; D000071480:APOBEC-3G Deaminase; C464924:APOBEC3G protein, human; D003564:Cytidine Deaminase