title: Antiviral Activity of Hederasaponin B from Hedera helix against Enterovirus 71 Subgenotypes C3 and C4a.
abstract: Enterovirus 71 (EV71) is the predominant cause of hand, foot and mouth disease (HFMD). The antiviral activity of hederasaponin B from Hedera helix against EV71 subgenotypes C3 and C4a was evaluated in vero cells. In the current study, the antiviral activity of hederasaponin B against EV71 C3 and C4a was determined by cytopathic effect (CPE) reduction method and western blot assay. Our results demonstrated that hederasaponin B and 30% ethanol extract of Hedera helix containing hederasaponin B showed significant antiviral activity against EV71 subgenotypes C3 and C4a by reducing the formation of a visible CPE. Hederasaponin B also inhibited the viral VP2 protein expression, suggesting the inhibition of viral capsid protein synthesis.These results suggest that hederasaponin B and Hedera helix extract containing hederasaponin B can be novel drug candidates with broad-spectrum antiviral activity against various subgenotypes of EV71.
authors.affiliation: Division of Vaccine Research, Center for Infectious Diseases, National Institute of Health, Korea Centers for Diseases Control and Prevention, Osong 363-951 ; College of Pharmacy, Kangwon National University, Chuncheon 200-701.
authors.forename: Jaehyoung
authors.initials: J
authors.lastname: Song
authors.affiliation: Division of Vaccine Research, Center for Infectious Diseases, National Institute of Health, Korea Centers for Diseases Control and Prevention, Osong 363-951 ; Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 151-742.
authors.forename: Sang-Gu
authors.initials: SG
authors.lastname: Yeo
authors.affiliation: College of Pharmacy, Kangwon National University, Chuncheon 200-701.
authors.forename: Eun-Hye
authors.initials: EH
authors.lastname: Hong
authors.affiliation: College of Pharmacy, Kangwon National University, Chuncheon 200-701.
authors.forename: Bo-Ra
authors.initials: BR
authors.lastname: Lee
authors.affiliation: Division of Vaccine Research, Center for Infectious Diseases, National Institute of Health, Korea Centers for Diseases Control and Prevention, Osong 363-951.
authors.forename: Jin-Won
authors.initials: JW
authors.lastname: Kim
authors.affiliation: Ahn-Gook Pharmaceutical Co., LTD, Seoul 150-953, Republic of Korea.
authors.forename: Jeonghoon
authors.initials: J
authors.lastname: Kim
authors.affiliation: Ahn-Gook Pharmaceutical Co., LTD, Seoul 150-953, Republic of Korea.
authors.forename: Hyeongun
authors.initials: H
authors.lastname: Jeong
authors.affiliation: College of Pharmacy, Kangwon National University, Chuncheon 200-701.
authors.forename: Yongsoo
authors.initials: Y
authors.lastname: Kwon
authors.affiliation: College of Pharmacy, Kangwon National University, Chuncheon 200-701.
authors.forename: Hyunpyo
authors.initials: H
authors.lastname: Kim
authors.affiliation: Division of Vaccine Research, Center for Infectious Diseases, National Institute of Health, Korea Centers for Diseases Control and Prevention, Osong 363-951.
authors.forename: Sangwon
authors.initials: S
authors.lastname: Lee
authors.affiliation: Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 151-742.
authors.forename: Jae-Hak
authors.initials: JH
authors.lastname: Park
authors.affiliation: College of Pharmacy, Kangwon National University, Chuncheon 200-701.
authors.forename: Hyun-Jeong
authors.initials: HJ
authors.lastname: Ko
keywords: Antiviral activity; Enterovirus 71; Hand foot and mouth disease; Hedera helix; Hederasaponin B