title: Zebrafish yap1 plays a role in differentiation of hair cells in posterior lateral line.
abstract: The evolutionarily conserved Hippo signaling pathway controls organ size by regulating cell proliferation and apoptosis and this process involves Yap1. The zebrafish Yap1 acts during neural differentiation, but its function is not fully understood. The detailed analysis of yap1 expression in proliferative regions, revealed it in the otic placode that gives rise to the lateral line system affected by the morpholino-mediated knockdown of Yap1. The comparative microarray analysis of transcriptome of Yap1-deficient embryos demonstrated changes in expression of many genes, including the Wnt signaling pathway and, in particular, prox1a known for its role in development of mechanoreceptors in the lateral line. The knockdown of Yap1 causes a deficiency of differentiation of mechanoreceptors, and this defect can be rescued by prox1a mRNA. Our studies revealed a role of Yap1 in regulation of Wnt signaling pathway and its target Prox1a during differentiation of mechanosensory cells.
authors.affiliation: Institute of Molecular and Cell Biology, Singapore.
authors.forename: Siau-Lin
authors.initials: SL
authors.lastname: Loh
authors.affiliation: Institute of Molecular and Cell Biology, Singapore.
authors.forename: Cathleen
authors.initials: C
authors.lastname: Teh
authors.affiliation: Institute of Molecular and Cell Biology, Singapore.
authors.forename: Julius
authors.initials: J
authors.lastname: Muller
authors.affiliation: Institute of Molecular and Cell Biology, Singapore.
authors.forename: Ernesto
authors.initials: E
authors.lastname: Guccione
authors.affiliation: 1] Institute of Molecular and Cell Biology, Singapore [2] Department of Biochemistry, National University of Singapore, Singapore.
authors.forename: Wanjin
authors.initials: W
authors.lastname: Hong
authors.affiliation: 1] Institute of Molecular and Cell Biology, Singapore [2] Department of Biological Sciences, National University of Singapore, Singapore.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Korzh
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D047108:Embryonic Development / Q000235:genetics; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D006198:Hair Cells, Auditory / Q000166:cytology* / Q000378:metabolism*; D011485:Protein Binding; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology / Q000235:genetics* / Q000378:metabolism
chemical_list: D014157:Transcription Factors