title: Sinomenine reverses multidrug resistance in bladder cancer cells via P-glycoprotein-dependent and independent manners.
abstract: P-Glycoprotein-mediated multidrug resistance is a frequent event during chemotherapy and a key obstacle for bladder cancer therapy. Search for strategies to reverse multidrug resistance is a promising approach to improve the management of bladder cancer. In the present study, we reported a novel P-glycoprotein-mediated multidrug resistant cell model 253J/DOX, which was generated from human bladder cancer 253J cell line. Furthermore, we found that the multidrug resistant phenotype of 253J/DOX cells could be overcome by sinomenine, an alkaloid derived from the stem of Sinomenium acutum. Mechanistically, the chemosensitive effect by sinomenine was mediated by down-regulating P-glycoprotein expression, as well as triggering apoptotic pathways. The chemosensitive effect of sinomenine may make it a prime candidate agent to target bladder cancer.
authors.affiliation: Department of Urology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, PR China.
authors.forename: Yule
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Urology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, PR China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, PR China.
authors.forename: Xinlan
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Urology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, PR China.
authors.forename: Kaijie
authors.initials: K
authors.lastname: Wu
authors.affiliation: Department of Urology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zeng
authors.affiliation: Department of Urology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Urology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Shi
authors.affiliation: Oncology Research Laboratory, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an, PR China.
authors.forename: Xinyang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Urology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, PR China.
authors.forename: Luke S
authors.initials: LS
authors.lastname: Chang
authors.affiliation: Department of Urology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, PR China.
authors.forename: Dalin
authors.initials: D
authors.lastname: He
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D045744:Cell Line, Tumor; D004396:Coloring Agents; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D006801:Humans; D007202:Indicators and Reagents; D009019:Morphinans / Q000494:pharmacology*; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020112:Rhodamine 123; D013778:Tetrazolium Salts; D013844:Thiazoles; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy*
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D004396:Coloring Agents; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D009019:Morphinans; D034741:RNA, Small Interfering; D013778:Tetrazolium Salts; D013844:Thiazoles; D020112:Rhodamine 123; C009271:sinomenine; C022616:thiazolyl blue