title: Genome- and transcriptome-assisted development of nuclear insertion/deletion markers for Calanus species (Copepoda: Calanoida) identification.
abstract: Copepods of the genus Calanus are key zooplankton species in temperate to arctic marine ecosystems. Despite their ecological importance, species identification remains challenging. Furthermore, the recent report of hybrids among Calanus species highlights the need for diagnostic nuclear markers to efficiently identify parental species and hybrids. Using next-generation sequencing analysis of both the genome and transcriptome from two sibling species, Calanus finmarchicus and Calanus glacialis, we developed a panel of 12 nuclear insertion/deletion markers. All the markers showed species-specific amplicon length. Furthermore, most of the markers were successfully amplified in other Calanus species, allowing the molecular identification of Calanus helgolandicus, Calanus hyperboreus and Calanus marshallae.
authors.affiliation: Faculty of Biosciences and Aquaculture, University of Nordland, 8049, Bodø, Norway.
authors.forename: I
authors.initials: I
authors.lastname: Smolina
authors.forename: S
authors.initials: S
authors.lastname: Kollias
authors.forename: M
authors.initials: M
authors.lastname: Poortvliet
authors.forename: T G
authors.initials: TG
authors.lastname: Nielsen
authors.forename: P
authors.initials: P
authors.lastname: Lindeque
authors.forename: C
authors.initials: C
authors.lastname: Castellani
authors.forename: E F
authors.initials: EF
authors.lastname: Møller
authors.forename: L
authors.initials: L
authors.lastname: Blanco-Bercial
authors.forename: G
authors.initials: G
authors.lastname: Hoarau
mesh_terms: D000818:Animals; D033342:Copepoda / Q000145:classification* / Q000235:genetics*; D005819:Genetic Markers; D016678:Genome; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D059467:Transcriptome
chemical_list: D005819:Genetic Markers
keywords: InDels; Ion Torrent; marker design; next-generation sequencing; species delimitation; zooplankton