title: Crystallization and preliminary X-ray diffraction analysis of the diterpene cyclooctatin synthase (CYC) from Streptomyces sp. LZ35.
abstract: Terpenoids are a large and highly diverse group of natural products, with the most chemically diverse pool of structures. Terpene synthase is the key enzyme in the process of terpenoid synthesis. In this paper, the first diterpene synthase (CYC) of bacterial origin was successfully crystallized. Native and SeMet-derivative crystals diffracted to 1.75 and 2.6 Å resolution, respectively. The native crystal belonged to space group P212121, with unit-cell parameters a = 59.10, b = 101.73, c = 108.93 Å, and contained two molecules per asymmetric unit. The SeMet-derivative crystal belonged to space group P21, with unit-cell parameters a = 58.64, b = 109.47, c = 58.73 Å, β = 119.35°, and had two molecules per asymmetric unit.
authors.affiliation: Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong 250012, People's Republic of China.
authors.forename: Xiulei
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, Jinan, Shandong 250100, People's Republic of China.
authors.forename: Guijun
authors.initials: G
authors.lastname: Shang
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, Jinan, Shandong 250100, People's Republic of China.
authors.forename: Lichuan
authors.initials: L
authors.lastname: Gu
authors.affiliation: Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong 250012, People's Republic of China.
authors.forename: Yuemao
authors.initials: Y
authors.lastname: Shen
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D019757:Carbon-Oxygen Lyases / Q000737:chemistry* / Q000302:isolation & purification; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D013302:Streptomyces / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D019757:Carbon-Oxygen Lyases
keywords: Streptomyces sp. LZ35; diterpene cyclooctatin synthase (CYC); terpene synthases