title: Reticulocyte maturity indices in iron deficiency anemia.
abstract: OBJECTIVE
  The aim of this study was to analyze the reticulocyte maturity indices (low, medium, and high fluorescence ratios) in iron deficient 1- to 6-year-old children, and identify the prevalence of iron deficiency anemia in this population.
  
  
  METHODS
  The present study included 39 subjects, divided into two groups: control subjects (n = 33), and subjects with iron deficiency anemia (n = 6). The results were analyzed by Student's t-test for comparison of means. Differences were considered significant when two-tailed p-value < 0.05.
  
  
  RESULTS
  Subjects with iron deficiency anemia presented increases in the proportion of mean (10.3 ± 4.7% vs. 6.0 ± 3.4%; p-value = 0.003), and high fluorescence reticulocytes (2.3 ± 0.87% vs. 0.9 ± 0.9%; p-value = 0.03) compared to the control group. The prevalence of anemia in this population was 15% (n = 6).
  
  
  CONCLUSIONS
  The indices related to immaturity of reticulocytes are higher in the presence of iron deficiency, thus demonstrating a deficiency in the raw material to form hemoglobin and are, therefore, possible early markers of iron deficiency and anemia. We emphasize the need to standardize these indices for use in clinical practice and lab test results.
authors.affiliation: Laboratório de Análises Clínicas J&R, Candelária, RS, Brazil.
authors.forename: Muriel
authors.initials: M
authors.lastname: Wollmann
authors.affiliation: Hemocentro Regional de Pelotas, Pelotas, RS, Brazil.
authors.forename: Branca Maria Cerezer
authors.initials: BM
authors.lastname: Gerzson
authors.affiliation: Universidade de Santa Cruz do Sul (UNISC), Santa Cruz, RS, Brazil.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Schwert
authors.affiliation: Universidade Luterana do Brasil (ULBRA), Cachoeira do Sul, RS, Brazil.
authors.forename: Rafael Weber
authors.initials: RW
authors.lastname: Figuera
authors.affiliation: Universidade Luterana do Brasil (ULBRA), Cachoeira do Sul, RS, Brazil.
authors.forename: Guilherme de Oliveira
authors.initials: Gde O
authors.lastname: Ritzel
keywords: Child; Iron deficiency; Reticulocytes