title: Pharmacokinetics study of asperosaponin VI and its metabolites cauloside A, HN saponin F and hederagenin.
abstract: This experiment's with aim was to study the pharmacokinetics of asperosaponin VI and its three metabolites (cauloside A, HN saponin F and hederagenin) via a sensitive high performance liquid chromatography connected with electrospray ionization triple quadrupole mass spectrum (HPLC-ESI-MS/MS). Chromatographic separation was achieved on a reverse phase C18 column with a gradient mobile phase of CH3CN-water with 0.1 % HCOOH at a flow rate of 0.3 mL/min. Sample analysis was simultaneously performed with a multiple reaction monitoring mode using target determination ions at m/z 927.5 → 603.4 for asperosaponin VI, m/z 811.1 → 603.4 for cauloside A, m/z 649.4 → 603.4 for HN saponin F, m/z 71.4 → 393.3 for hederagenin and m/z 307.0 → 161.1 for warfarin as the internal standard. The calibration curve was linear at the range of 0.25-500 ng/mL, and the lower limit of quantification was 0.25 ng/mL for each compound. While the precisely intra-assay and inter-assay variabilities were <9.5 and 7.8 %, respectively; accuracy was determined at the concentrations of 5, 25, 100 ng/mL for all the analytes with the relative standard deviation (%) no more than 15.0 %. Consequently, the validated method could be successfully and precisely applied to the pharmacokinetic study of asperosaponin VI and its metabolites. As a result, the pharmacokinetic parameters of cauloside A, HN saponin F and hederagenin such as T max were obtained at 9.33 ± 2.49, 7.33 ± 0.47 and 12.33 ± 2.36 h, respectively.
authors.affiliation: Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, 88 Yuquan Road, Nankai District, Tianjin, 300193, China.
authors.forename: Er-Wei
authors.initials: EW
authors.lastname: Liu
authors.forename: Jia-Long
authors.initials: JL
authors.lastname: Wang
authors.forename: Li-Feng
authors.initials: LF
authors.lastname: Han
authors.forename: Yan-Xu
authors.initials: YX
authors.lastname: Chang
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Huo
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Xiu-Mei
authors.initials: XM
authors.lastname: Gao
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008297:Male; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000737:chemistry / Q000493:pharmacokinetics*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: C000590802:HN saponin F; D012503:Saponins; C534004:akebia saponin D; C036411:cauloside A; D009828:Oleanolic Acid; C025763:hederagenin