title: Assignments of RNase A by ADAPT-NMR and enhancer.
abstract: We report here backbone (1)H and (15)N assignments for ribonuclease A obtained by using ADAPT-NMR, a fully-automated approach for combined data collection, spectral analysis and resonance assignment. ADAPT-NMR was able to assign 98% of the resonances with 93% agreement with traditional data collection and assignment. Further refinement of the automated results with ADAPT-NMR enhancer led to complete (100%) assignments with 96% agreement with assignments by the traditional approach.
authors.affiliation: National Magnetic Resonance Facility at Madison, University of Wisconsin - Madison, 433 Babcock Drive, Madison, WI, 53706, USA, tonelli@nmrfam.wisc.edu.
authors.forename: Marco
authors.initials: M
authors.lastname: Tonelli
authors.forename: Chelcie H
authors.initials: CH
authors.lastname: Eller
authors.forename: Kiran K
authors.initials: KK
authors.lastname: Singarapu
authors.forename: Woonghee
authors.initials: W
authors.lastname: Lee
authors.forename: Arash
authors.initials: A
authors.lastname: Bahrami
authors.forename: William M
authors.initials: WM
authors.lastname: Westler
authors.forename: Ronald T
authors.initials: RT
authors.lastname: Raines
authors.forename: John L
authors.initials: JL
authors.lastname: Markley
mesh_terms: D000818:Animals; D002417:Cattle; D019906:Nuclear Magnetic Resonance, Biomolecular; D010710:Phosphates / Q000494:pharmacology; D012259:Ribonuclease, Pancreatic / Q000737:chemistry*
chemical_list: D010710:Phosphates; D012259:Ribonuclease, Pancreatic