title: Polymorphisms in DNA repair genes (APEX1, XPD, XRCC1 and XRCC3) and risk of preeclampsia in a Mexican mestizo population.
abstract: Variations in genes involved in DNA repair systems have been proposed as risk factors for the development of preeclampsia (PE). We conducted a case-control study to investigate the association of Human apurinic/apyrimidinic (AP) endonuclease (APEX1) Asp148Glu (rs1130409), Xeroderma Pigmentosum group D (XPD) Lys751Gln (rs13181), X-ray repair cross-complementing group 1 (XRCC) Arg399Gln (rs25487) and X-ray repair cross-complementing group 3 (XRCC3) Thr241Met (rs861539) polymorphisms with PE in a Mexican population. Samples of 202 cases and 350 controls were genotyped using RTPCR. Association analyses based on a χ2 test and binary logistic regression were performed to determine the odds ratio (OR) and a 95% confidence interval (95% CI) for each polymorphism. The allelic frequencies of APEX1 Asp148Glu polymorphism showed statistical significant differences between preeclamptic and normal women (p = 0.036). Although neither of the polymorphisms proved to be a risk factor for the disease, the APEX1 Asp148Glu polymorphism showed a tendency of association (OR: 1.74, 95% CI = 0.96-3.14) and a significant trend (p for trend = 0.048). A subgroup analyses revealed differences in the allelic frequencies of APEX1 Asp148Glu polymorphism between women with mild preeclampsia and severe preeclampsia (p = 0.035). In conclusion, our results reveal no association between XPD Lys751Gln, XRCC Arg399Gln and XRCC3 Thr241Met polymorphisms and the risk of PE in a Mexican mestizo population; however, the results in the APEX1 Asp148Glu polymorphism suggest the need for future studies using a larger sample size.
authors.affiliation: Institute for Scientific Research, Juarez University of Durango State, 34000 Durango, México. adda-sandoval@hotmail.com.
authors.forename: Ada
authors.initials: A
authors.lastname: Sandoval-Carrillo
authors.affiliation: Faculty of Medicine and Nutrition, Juarez University of Durango State, 34000 Durango, México. edna_madai@hotmail.com.
authors.forename: Edna M
authors.initials: EM
authors.lastname: Méndez-Hernández
authors.affiliation: General Hospital of Durango, Secretary of Health, Durango, 34000 Durango, México. feralaniz1@hotmail.com.
authors.forename: Fernando
authors.initials: F
authors.lastname: Vazquez-Alaniz
authors.affiliation: Institute for Scientific Research, Juarez University of Durango State, 34000 Durango, México. aguilarduran_marisela@hotmail.com.
authors.forename: Marisela
authors.initials: M
authors.lastname: Aguilar-Durán
authors.affiliation: Faculty of Medicine and Nutrition, Juarez University of Durango State, 34000 Durango, México. tellezalfredo@gmail.com.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Téllez-Valencia
authors.affiliation: Faculty of Medicine and Nutrition, Juarez University of Durango State, 34000 Durango, México. sbmaj30@hotmail.com.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Barraza-Salas
authors.affiliation: Institute for Scientific Research, Juarez University of Durango State, 34000 Durango, México. feralaniz1@hotmail.com.
authors.forename: Francisco X
authors.initials: FX
authors.lastname: Castellanos-Juárez
authors.affiliation: Institute for Scientific Research, Juarez University of Durango State, 34000 Durango, México. ollave56@yahoo.es.
authors.forename: Osmel La
authors.initials: OL
authors.lastname: Llave-León
authors.affiliation: Institute for Scientific Research, Juarez University of Durango State, 34000 Durango, México. jsalas_pacheco@hotmail.com.
authors.forename: José M
authors.initials: JM
authors.lastname: Salas-Pacheco
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D004260:DNA Repair / Q000235:genetics; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D016015:Logistic Models; D008800:Mexico; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D011225:Pre-Eclampsia / Q000235:genetics* / Q000473:pathology; D011247:Pregnancy; D012307:Risk Factors; D012720:Severity of Illness Index; D000076105:X-ray Repair Cross Complementing Protein 1; D051759:Xeroderma Pigmentosum Group D Protein / Q000235:genetics*; D055815:Young Adult
chemical_list: D004268:DNA-Binding Proteins; D000076105:X-ray Repair Cross Complementing Protein 1; C110901:X-ray repair cross complementing protein 3; C000619371:XRCC1 protein, human; D051759:Xeroderma Pigmentosum Group D Protein; C085108:APEX1 protein, human; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; C497292:ERCC2 protein, human