title: Optimization of total polysaccharide extraction from Herba Lophatheri using RSM and antioxidant activities.
abstract: Effects of extraction temperature, extraction time and ratio of water to material as well as their interactions on the yield of total polysaccharide from Herba lophatheri were studied by response surface methodology (RSM). The optimal conditions for the extraction of polysaccharides were determined to be the ratio of liquid to solid of 39.01, extraction time of 2.11h and extraction temperature of 97.09°C. Under these optimal conditions, the yield of polysaccharides obtained was 5.73±0.10%, which was well matched with the value predicted by the model. In vitro antioxidant assays showed that the polysaccharides HLP possessed significant inhibitory effects on superoxide radical. It also exhibited strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities, hydroxyl radical scavenging activities and its reducing power, ferrous ions chelating effect was also strong. These results suggested that H. lophatheri polysaccharides could be a suitable natural antioxidant and may be the functional foods for humans.
authors.affiliation: Key Laboratory of Chemical and Biological Processing Technology for Farm Products of Zhejiang Province, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, PR China. Electronic address: gq0318@163.com.
authors.forename: Qing
authors.initials: Q
authors.lastname: Ge
authors.affiliation: Key Laboratory of Chemical and Biological Processing Technology for Farm Products of Zhejiang Province, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Huang
authors.affiliation: Key Laboratory of Chemical and Biological Processing Technology for Farm Products of Zhejiang Province, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, PR China.
authors.forename: Jian-wei
authors.initials: JW
authors.lastname: Mao
authors.affiliation: Key Laboratory of Chemical and Biological Processing Technology for Farm Products of Zhejiang Province, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, PR China.
authors.forename: Jin-yan
authors.initials: JY
authors.lastname: Gong
authors.affiliation: Key Laboratory of Chemical and Biological Processing Technology for Farm Products of Zhejiang Province, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, PR China.
authors.forename: Yi-feng
authors.initials: YF
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Chemical and Biological Processing Technology for Farm Products of Zhejiang Province, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, PR China.
authors.forename: Jin-xin
authors.initials: JX
authors.lastname: Huang
mesh_terms: D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D016166:Free Radical Scavengers / Q000737:chemistry; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D006109:Poaceae / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D013499:Surface Properties; D014867:Water / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D010936:Plant Extracts; D011134:Polysaccharides; D014867:Water
keywords: Herba lophatheri; Polysaccharide; Response surface methodology