title: Structural and biochemical changes induced by pulsed electric field treatments on Cabernet Sauvignon grape berry skins: impact on cell wall total tannins and polysaccharides.
abstract: Pulsed electric field (PEF) treatment is an emerging technology that is arousing increasing interest in vinification processes for its ability to enhance polyphenol extraction performance. The aim of this study was to investigate the effects of PEF treatment on grape skin histocytological structures and on the organization of skin cell wall polysaccharides and tannins, which, until now, have been little investigated. This study relates to the effects of two PEF treatments on harvested Cabernet Sauvignon berries: PEF1 (medium strength (4 kV/cm); short duration (1 ms)) and PEF2 (low intensity (0.7 kV/cm); longer duration (200 ms)). Histocytological observations and the study of levels of polysaccharidic fractions and total amounts of tannins allowed differentiation between the two treatments. Whereas PEF1 had little effect on the polyphenol structure and pectic fraction, PEF2 profoundly modified the organization of skin cell walls. Depending on the PEF parameters, cell wall structure was differently affected, providing variable performance in terms of polyphenol extraction and wine quality.
authors.affiliation: Université de Bordeaux, Institut des Sciences de la Vigne et du Vin , EA 4577, Unité de recherche œnologie, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Cholet
authors.forename: Cristèle
authors.initials: C
authors.lastname: Delsart
authors.forename: Mélina
authors.initials: M
authors.lastname: Petrel
authors.forename: Etienne
authors.initials: E
authors.lastname: Gontier
authors.forename: Nabil
authors.initials: N
authors.lastname: Grimi
authors.forename: Annie
authors.initials: A
authors.lastname: L'hyvernay
authors.forename: Remy
authors.initials: R
authors.lastname: Ghidossi
authors.forename: Eugène
authors.initials: E
authors.lastname: Vorobiev
authors.forename: Martine
authors.initials: M
authors.lastname: Mietton-Peuchot
authors.forename: Laurence
authors.initials: L
authors.lastname: Gény
mesh_terms: D002473:Cell Wall / Q000737:chemistry*; D004560:Electricity; D005511:Food Handling; D005638:Fruit / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D013634:Tannins / Q000737:chemistry*; D027843:Vitis / Q000737:chemistry*; D014920:Wine / Q000032:analysis
chemical_list: D011134:Polysaccharides; D013634:Tannins