title: BDNF attenuates IL-1β-induced F-actin remodeling by inhibiting NF-κB signaling in hippocampal neurons.
abstract: OBJECTIVE
  To examine the effect of BDNF on F-actin during the stimulation of IL-1β in hippocampal neurons.
  
  
  METHODS
  We cultured hippocampal neurons from rat embryos. Cell stimulation was induced by IL-1β. Cell culture success was evaluated by an activity analysis of CCK-8, staining of gliocyte by immunohistochemistry. Changes in F-actin, BDNF and NF-ĸB were examined using molecular analyses.
  
  
  RESULTS
  Our results demonstrate that a high concentration of IL-1β exaggerates the stimulation-induced degradation of F-actin by BDNF, whereas a low concentration of IL-1β protects F-actin against this degradation. These beneficial effects might be associated with the inhibition or exaggeration of the NF-ĸB signaling cascade.
  
  
  CONCLUSIONS
  Taken together, our findings indicate that BDNF acts as an F-actin-protective regulator during stimulation by IL-1β and that this function largely occurs via the regulation of NF- ĸB signaling. These results suggest that interventions targeting the BDNF signaling system may be of therapeutic value against major depressive disorder (MDD).
authors.affiliation: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, China. wangghwh@tom.com.
authors.forename: Zhongxiang
authors.initials: Z
authors.lastname: Cai
authors.affiliation: Department of Psychiatry, Renmin Hospital, Wuhan University, Jiefang Road 238#, Wuhan 430060, China.
authors.forename: Xueping
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Psychiatry, Renmin Hospital, Wuhan University, Jiefang Road 238#, Wuhan 430060, China. wangghwh@tom.com.
authors.forename: Gaohua
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Psychiatry, Renmin Hospital, Wuhan University, Jiefang Road 238#, Wuhan 430060, China.
authors.forename: Huiling
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Psychiatry, Renmin Hospital, Wuhan University, Jiefang Road 238#, Wuhan 430060, China.
authors.forename: Zhongchun
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Psychiatry, Renmin Hospital, Wuhan University, Jiefang Road 238#, Wuhan 430060, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Psychiatry, Renmin Hospital, Wuhan University, Jiefang Road 238#, Wuhan 430060, China.
authors.forename: Can
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Psychiatry, Renmin Hospital, Wuhan University, Jiefang Road 238#, Wuhan 430060, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Psychiatry, Renmin Hospital, Wuhan University, Jiefang Road 238#, Wuhan 430060, China.
authors.forename: Hesheng
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Psychiatry, Renmin Hospital, Wuhan University, Jiefang Road 238#, Wuhan 430060, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Shu
authors.affiliation: Department of Psychiatry, Renmin Hospital, Wuhan University, Jiefang Road 238#, Wuhan 430060, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Xiao
mesh_terms: D000199:Actins / Q000737:chemistry*; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000502:physiology*; D005260:Female; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D053583:Interleukin-1beta / Q000502:physiology*; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D000199:Actins; D019208:Brain-Derived Neurotrophic Factor; D053583:Interleukin-1beta; D016328:NF-kappa B