title: Identification and differentiation of Candida parapsilosis complex species by use of exon-primed intron-crossing PCR.
abstract: The Candida parapsilosis complex is composed of Candida parapsilosis sensu stricto, Candida orthopsilosis, Candida metapsilosis, and the closely related species Lodderomyces elongisporus. An exon-primed intron-crossing PCR assay was developed here to distinguish the members of the species complex on the basis of the distinct sizes of amplicons, and Candida orthopsilosis and Candida metapsilosis were further discriminated by restriction enzyme analysis.
authors.affiliation: Medical Mycology Laboratory, Department of Dermatology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Feng
authors.forename: Zengbin
authors.initials: Z
authors.lastname: Wu
authors.forename: Bo
authors.initials: B
authors.lastname: Ling
authors.forename: Shuming
authors.initials: S
authors.lastname: Pan
authors.forename: Wanqing
authors.initials: W
authors.lastname: Liao
authors.forename: Weihua
authors.initials: W
authors.lastname: Pan
authors.forename: Zhirong
authors.initials: Z
authors.lastname: Yao
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D001483:Base Sequence; D002175:Candida / Q000187:drug effects / Q000235:genetics*; D002177:Candidiasis / Q000175:diagnosis* / Q000382:microbiology*; D005091:Exons / Q000235:genetics*; D007438:Introns / Q000235:genetics*; D008826:Microbial Sensitivity Tests / Q000379:methods; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D016415:Sequence Alignment
chemical_list: D000935:Antifungal Agents