title: Constructing a new integrated genetic linkage map and mapping quantitative trait loci for vegetative mycelium growth rate in Lentinula edodes.
abstract: The most saturated linkage map for Lentinula edodes to date was constructed based on a monokaryotic population of 146 single spore isolates (SSIs) using sequence-related amplified polymorphism (SRAP), target region amplification polymorphism (TRAP), insertion-deletion (InDel) markers, and the mating-type loci. Five hundred and twenty-four markers were located on 13 linkage groups (LGs). The map spanned a total length of 1006.1 cM, with an average marker spacing of 2.0 cM. Quantitative trait loci (QTLs) mapping was utilized to uncover the loci regulating and controlling the vegetative mycelium growth rate on various synthetic media, and complex medium for commercial cultivation of L. edodes. Two and 13 putative QTLs, identified respectively in the monokaryotic population and two testcross dikaryotic populations, were mapped on seven different LGs. Several vegetative mycelium growth rate-related QTLs uncovered here were clustered on LG4 (Qmgr1, Qdgr1, Qdgr2 and Qdgr9) and LG6 (Qdgr3, Qdgr4 and Qdgr5), implying the presence of main genomic areas responsible for growth rate regulation and control. The QTL hotspot region on LG4 was found to be in close proximity to the region containing the mating-type A (MAT-A) locus. Moreover, Qdgr2 on LG4 was detected on different media, contributing 8.07 %-23.71 % of the phenotypic variation. The present study provides essential information for QTL mapping and marker-assisted selection (MAS) in L. edodes.
authors.affiliation: Key Laboratory of Agro-Microbial Resource and Development (Ministry of Agriculture), Huazhong Agricultural University, Wuhan 430070, Hubei Province, PR China; Institute of Applied Mycology, Huazhong Agricultural University, Hubei Province 430070, PR China.
authors.forename: Wen-Bing
authors.initials: WB
authors.lastname: Gong
authors.affiliation: Institute of Applied Mycology, Huazhong Agricultural University, Hubei Province 430070, PR China; Institute of Hydrobiology, Chinese Academy of Sciences, Hubei Province 430072, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Institute of Applied Mycology, Huazhong Agricultural University, Hubei Province 430070, PR China; Institute of Crop Genetic Resource, Guizhou Academy of Agricultural Sciences, Guiyang 550006, Guizhou Province, PR China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Lu
authors.affiliation: Key Laboratory of Agro-Microbial Resource and Development (Ministry of Agriculture), Huazhong Agricultural University, Wuhan 430070, Hubei Province, PR China; Institute of Applied Mycology, Huazhong Agricultural University, Hubei Province 430070, PR China.
authors.forename: Yin-Bing
authors.initials: YB
authors.lastname: Bian
authors.affiliation: Key Laboratory of Agro-Microbial Resource and Development (Ministry of Agriculture), Huazhong Agricultural University, Wuhan 430070, Hubei Province, PR China; Institute of Applied Mycology, Huazhong Agricultural University, Hubei Province 430070, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, PR China.
authors.forename: Hoi Shan
authors.initials: HS
authors.lastname: Kwan
authors.affiliation: School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, PR China.
authors.forename: Man Kit
authors.initials: MK
authors.lastname: Cheung
authors.affiliation: Key Laboratory of Agro-Microbial Resource and Development (Ministry of Agriculture), Huazhong Agricultural University, Wuhan 430070, Hubei Province, PR China; Institute of Applied Mycology, Huazhong Agricultural University, Hubei Province 430070, PR China. Electronic address: xysubmission@gmail.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xiao
mesh_terms: D002874:Chromosome Mapping; D003470:Culture Media / Q000737:chemistry; D008040:Genetic Linkage; D025282:Mycelium / Q000235:genetics* / Q000254:growth & development*; D040641:Quantitative Trait Loci; D020942:Shiitake Mushrooms / Q000235:genetics* / Q000254:growth & development*
chemical_list: D003470:Culture Media
keywords: Genetic linkage map; Quantitative trait loci; Shiitake mushroom; Vegetative mycelium growth rate