title: Plastid transformation in soybean.
abstract: The biotechnological potential of plastid genetic engineering has been illustrated in a limited number of higher plant species. We have developed a reproducible method to generate plastid transformants in soybean (Glycine max), a crop of major agronomic importance. The transformation vectors are delivered to embryogenic cultures by the particle gun method and selection performed using the aadA antibiotic resistance gene. Homoplasmy is established rapidly in the selected events without the need for further selection or regeneration cycles, and genes of interest can be expressed at a high level in green tissues. This is a significant step toward the commercial application of this technology.
authors.affiliation: BioScience, Bayer CropScience, Morrisville, NC, USA.
authors.forename: Manuel
authors.initials: M
authors.lastname: Dubald
authors.forename: Ghislaine
authors.initials: G
authors.lastname: Tissot
authors.forename: Bernard
authors.initials: B
authors.lastname: Pelissier
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D019470:Biolistics / Q000379:methods*; D002736:Chloroplasts / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics; D009713:Nucleotidyltransferases / Q000235:genetics; D045332:Photosystem II Protein Complex / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics; D011401:Promoter Regions, Genetic; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012639:Seeds / Q000235:genetics; D013025:Glycine max / Q000235:genetics*; D000198:Spectinomycin / Q000494:pharmacology; D014170:Transformation, Genetic; D019076:Transgenes / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D004275:DNA, Ribosomal; D045332:Photosystem II Protein Complex; D012336:RNA, Ribosomal, 16S; D000198:Spectinomycin; D009713:Nucleotidyltransferases; C020565:gentamicin 2''-nucleotidyltransferase