title: The Comparison of The Effects of Silybin and Silybin-Phosphatidylcholine on Viability and ESR Expression in Human Breast Cancer T47D Cell Line.
abstract: OBJECTIVE
  Silybin is a polyphenol with anti-oxidant and anti-cancer properties. The poor bioavailability of some polyphenols can be improved by binding to phosphatidylcholine. In recent years, studies have been conducted to evaluate the anti-cancer effect of silybin. We studied the effect of silybin and silybin-phosphatidylcholine on ESR1 and ESR2 gene expression and viability in the T47D breast cancer cell line.
  
  
  METHODS
  In this experimental study, a 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide test (MTT test) was used to determine doses for cell treatment, and the gene expression was analyzed by real-time reverse transcriptase-polymerase chain reaction (real-time RT- PCR).
  
  
  RESULTS
  Significant dose- and time-dependent cell growth inhibitory effects of silybin and silybin-phosphatidylcholine along with ESR1 down-regulation were observed in T47D cells. In contrast to ESR1, the T47D cell line showed negligible ESR2 expression.
  
  
  CONCLUSIONS
  This study suggests that silybin and silybin-phosphatidylcholine down-regulate ESR1 in ER(+)breast cancers. Results also show that in the T47D cell line, silybindown-regulation of ESR1 compared with silybin.
authors.affiliation: Department of Cell and Molecular Biology, Kish International Campus, University of Tehran, Kish, Iran ; School of Biology, University College of Science, University of Tehran, Tehran, Iran.
authors.forename: Narges
authors.initials: N
authors.lastname: Mahmoodi
authors.affiliation: Department of Cell and Molecular Biology, Kish International Campus, University of Tehran, Kish, Iran ; School of Biology, University College of Science, University of Tehran, Tehran, Iran.
authors.forename: Nasrin
authors.initials: N
authors.lastname: Motamed
authors.affiliation: School of Biology, Damghan University, Damghan, Iran.
authors.forename: Seyed Hassan
authors.initials: SH
authors.lastname: Paylakhi
keywords: Breast Cancer; ESR1; Silybin; Silybin-Phosphatidylcholine