title: Sec16 determines the size and functioning of the Golgi in the protist parasite, Trypanosoma brucei.
abstract: The Sec16 homologue in Trypanosoma brucei has been identified and characterized. TbSec16 colocalizes with COPII components at the single endoplasmic reticulum exit site (ERES), which is next to the single Golgi stack in the insect (procyclic) form of this organism. Depletion of TbSec16 reduces the size of the ERES and the Golgi, and slows growth and transport of a secretory marker to the cell surface; conversely, overexpression of TbSec16 increases the size of the ERES and Golgi but has no effect on growth or secretion. Together these data suggest that TbSec16 regulates the size of the ERES and Golgi and this size is set for optimal growth of the organism.
authors.affiliation: Max F. Perutz Laboratories, University of Vienna and Medical University of Vienna, Dr. Bohr-Gasse 9/3, 1030, Vienna, Austria.
authors.forename: Marco
authors.initials: M
authors.lastname: Sealey-Cardona
authors.forename: Katy
authors.initials: K
authors.lastname: Schmidt
authors.forename: Lars
authors.initials: L
authors.lastname: Demmel
authors.forename: Tatjana
authors.initials: T
authors.lastname: Hirschmugl
authors.forename: Tanja
authors.initials: T
authors.lastname: Gesell
authors.forename: Gang
authors.initials: G
authors.lastname: Dong
authors.forename: Graham
authors.initials: G
authors.lastname: Warren
mesh_terms: D006056:Golgi Apparatus / Q000378:metabolism*; D021381:Protein Transport; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D055571:Secretory Pathway; D014346:Trypanosoma brucei brucei / Q000235:genetics / Q000378:metabolism*; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D015800:Protozoan Proteins; D033921:Vesicular Transport Proteins
keywords: ER exit site; Golgi; TbSec16; Trypanosoma brucei