title: An iron-acquisition-deficient mutant of Corynebacterium pseudotuberculosis efficiently protects mice against challenge.
abstract: Caseous lymphadenitis (CLA) is a chronic disease that affects sheep and goats worldwide, and its etiological agent is Corynebacterium pseudotuberculosis. Despite the economic losses caused by CLA, there is little information about the molecular mechanisms of bacterial pathogenesis, and current immune prophylaxis against infection has been unable to reduce the incidence of CLA in goats. Recently, 21 different mutant strains of C. pseudotuberculosis were identified by random mutagenesis. In this study, these previously generated mutants were used in mice vaccination trials to develop new immunogens against CLA. Based on this analysis, CZ171053, an iron-acquisition-deficient mutant strain, was selected. After challenge with a virulent strain, 80% of the animals that were immunized with the CZ171053 strain survived. Furthermore, this vaccination elicited both humoral and cellular responses. Intracellular survival of the bacterium was determined using murine J774 cells; in this assay, the CZ171053 had reduced intracellular viability. Because iron acquisition in intracellular bacteria is considered one of their most important virulence factors during infection, these results demonstrate the immunogenic potential of this mutant against CLA.
authors.forename: Dayana
authors.initials: D
authors.lastname: Ribeiro
authors.forename: Flávia de Souza
authors.initials: Fde S
authors.lastname: Rocha
authors.forename: Kátia Morais Costa
authors.initials: KM
authors.lastname: Leite
authors.forename: Siomar de Castro
authors.initials: Sde C
authors.lastname: Soares
authors.forename: Artur
authors.initials: A
authors.lastname: Silva
authors.forename: Ricardo Wagner Dias
authors.initials: RW
authors.lastname: Portela
authors.forename: Roberto
authors.initials: R
authors.lastname: Meyer
authors.forename: Anderson
authors.initials: A
authors.lastname: Miyoshi
authors.forename: Sérgio Costa
authors.initials: SC
authors.lastname: Oliveira
authors.affiliation: Laboratório de Genética Celular e Molecular, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av, Antônio Carlos, 6627 - Pampulha, CP 486, CEP 31, Belo Horizonte, MG 270-901, Brazil. vascoariston@gmail.com.
authors.forename: Vasco
authors.initials: V
authors.lastname: Azevedo
authors.forename: Fernanda Alves
authors.initials: FA
authors.lastname: Dorella
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D003354:Corynebacterium Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D016925:Corynebacterium pseudotuberculosis / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity*; D016207:Cytokines / Q000097:blood; D007136:Immunoglobulins / Q000097:blood; D008199:Lymphadenitis / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D014774:Virulence
chemical_list: D001428:Bacterial Vaccines; D016207:Cytokines; D007136:Immunoglobulins