title: Determination of M/G ratio of propylene glycol alginate sodium sulfate by HPLC with pre-column derivatization.
abstract: A reliable high performance liquid chromatography with pre-column derivatization method was developed for the determination of the mannuronic acid (M)/guluronic acid (G) ratio of propylene glycol alginate sodium sulfate (PSS). The hydrolysis conditions of PSS were investigated by four degradation methods based on the degree of destruction of M and G, and the chromatographic separation conditions were also optimized. A satisfactory resolution of M and G was achieved with a KP-C18 column using 0.1 mol/L phosphate buffer (pH 7.0)-acetonitrile (83/17, v/v) as a mobile phase, after PSS was hydrolyzed with 0.1 mol/L sulfuric acid and labeled with 1-phenyl-3-methyl -5-pyrazolone. The M/G ratio of PSS determined by this method was in good accordance with that obtained by the (1)H NMR method with a desulfurization strategy. Our method is rapid, sensitive, accurate and reproducible. The limit of detection was found to be 0.25 μg/mL for M and 0.40 μg/mL for G.
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, Ocean University of China, Qingdao 266003, China. Electronic address: zhaoxia@ouc.edu.cn.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Qingdao Chiatai Haier Pharmaceutical Co., Ltd, Qingdao 266103, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ren
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
authors.forename: Yiting
authors.initials: Y
authors.lastname: Xue
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, Ocean University of China, Qingdao 266003, China.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Li
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, Ocean University of China, Qingdao 266003, China.
authors.forename: Guangli
authors.initials: G
authors.lastname: Yu
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, Ocean University of China, Qingdao 266003, China.
authors.forename: Huashi
authors.initials: H
authors.lastname: Guan
mesh_terms: D000464:Alginates / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006603:Hexuronic Acids / Q000737:chemistry*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; C007896:guluronic acid; C038550:propylene glycol alginate ester; C008324:mannuronic acid
keywords: 1-Phenyl-3-methyl-5-pyrazolone.; Hydrolysis; M/G ratio; Pre-column derivatization; Propylene glycol alginate sodium sulfate