title: Electrochemical immunosensor based on hyperbranched structure for carcinoembryonic antigen detection.
abstract: Sensitive determination of carcinoembryonic antigen (CEA) is very important in clinical research and diagnosis. Herein we report the design and synthesis of a new kind of immunosensor based on the benefits of hyperbranched structure. The hyperbranched polyester was grafted to the surface of indium tin oxides glass (ITO) electrode, and the grafting processes were characterized by attentuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). After CEA and horse radish peroxidase (HRP)-labeled antibody-conjugated AuNPs (HRP-Ab2-AuNPs) bioconjugates were immobilized on the surface of the hyperbranched structure-modified electrode, the optimized conditions of the above electrode were investigated. Moreover, the analytical performance of the proposed immunosensor showed a high sensitivity, a linear range from 0.01 to 80ng/mL with a low detection limit of 2.36pg/mL, and good selectivity for CEA. The designed immunoassay system holds great potential for ultrasensitive electrochemical biosensing of other analytes.
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Miao
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Wang
authors.affiliation: Ten D Nurse Station, Nanjing BenQ Hospital Co., Ltd., Nanjing 210019, China.
authors.forename: Liandi
authors.initials: L
authors.lastname: Lu
authors.affiliation: Department of Blood Transfusion, Nanjing Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China.
authors.forename: Peiyuan
authors.initials: P
authors.lastname: Zhu
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China. Electronic address: maochun127@aliyun.com.
authors.forename: Chun
authors.initials: C
authors.lastname: Mao
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.
authors.forename: Haolin
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.
authors.forename: Youchao
authors.initials: Y
authors.lastname: Song
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China. Electronic address: jshen@njnu.edu.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Shen
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D002272:Carcinoembryonic Antigen / Q000032:analysis* / Q000276:immunology; D003217:Conductometry / Q000295:instrumentation*; D050091:Dendrimers; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D008832:Microchemistry / Q000295:instrumentation*
chemical_list: D002272:Carcinoembryonic Antigen; D050091:Dendrimers
keywords: CEA detection; Electrochemical immunosensor; Hyperbranched structure