title: Quantitative investigation of calcimimetic R568 on beta cell adhesion and mechanics using AFM single-cell force spectroscopy.
abstract: In this study we use a novel approach to quantitatively investigate mechanical and interfacial properties of clonal β-cells using AFM-Single Cell Force Spectroscopy (SCFS). MIN6 cells were incubated for 48 h with 0.5 mM Ca(2+) ± the calcimimetic R568 (1 μM). AFM-SCFS adhesion and indentation experiments were performed by using modified tipless cantilevers. Hertz contact model was applied to analyse force-displacement (F-d) curves for determining elastic or Young's modulus (E). Our results show CaSR-evoked increases in cell-to-cell adhesion parameters and E modulus of single cells, demonstrating that cytomechanics have profound effects on cell adhesion characterization.
authors.affiliation: School of Engineering, University of Warwick, CV4 7AL, UK.
authors.forename: Eleftherios
authors.initials: E
authors.lastname: Siamantouras
authors.affiliation: School of Life Sciences, University of Warwick, CV4 7AL, UK.
authors.forename: Claire E
authors.initials: CE
authors.lastname: Hills
authors.affiliation: School of Life Sciences, University of Warwick, CV4 7AL, UK.
authors.forename: Mustafa Y G
authors.initials: MY
authors.lastname: Younis
authors.affiliation: School of Life Sciences, University of Warwick, CV4 7AL, UK.
authors.forename: Paul E
authors.initials: PE
authors.lastname: Squires
authors.affiliation: School of Engineering, University of Warwick, CV4 7AL, UK. Electronic address: I.K.Liu@warwick.ac.uk.
authors.forename: Kuo-Kang
authors.initials: KK
authors.lastname: Liu
mesh_terms: D000814:Aniline Compounds / Q000494:pharmacology*; D000818:Animals; D001696:Biomechanical Phenomena; D002448:Cell Adhesion; D002460:Cell Line; D055119:Elastic Modulus / Q000187:drug effects; D050417:Insulin-Secreting Cells / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D018625:Microscopy, Atomic Force; D010627:Phenethylamines; D011437:Propylamines; D044169:Receptors, Calcium-Sensing / Q000819:agonists
chemical_list: D000814:Aniline Compounds; C107873:N-(2-chlorophenylpropyl)-1-(3-methoxyphenyl)ethylamine; D010627:Phenethylamines; D011437:Propylamines; D044169:Receptors, Calcium-Sensing
keywords: AFM-SCFS; CaSR; Calcimimetic; Cell adhesion; Elasticity; Indentation