title: Effects of a novel SNP of IGF2R gene on growth traits and expression rate of IGF2R and IGF2 genes in gluteus medius muscle of Egyptian buffalo.
abstract: Insulin-like growth factor 2 receptor (IGF2R) is responsible for degradation of the muscle development initiator, IGF2, and thus it can be used as a marker for selection strategies in the farm animals. The aim of this study was to search for polymorphisms in three coding loci of IGF2R, and to analyze their effect on the growth traits and on the expression levels of IGF2R and IGF2 genes in the gluteus medius muscle of Egyptian buffaloes. A novel A266C SNP was detected in the coding sequences of the third IGF2R locus (at nucleotide number 51 of exon 23) among Egyptian water buffaloes. This SNP was non-synonymous mutation and led to replacement of Y (tyrosine) amino acid (aa) by D (aspartic acid) aa. Three different single-strand conformation polymorphism patterns were observed in the third IGF2R locus: AA, AC, and CC with frequencies of 0.555, 0.195, and 0.250, respectively. Statistical analysis showed that the homozygous AA genotype significantly associated with the average daily gain than AC and CC genotypes from birth to 9 mo of age. Expression analysis showed that the A266C SNP was correlated with IGF2, but not with IGF2R, mRNA levels in the gluteus medius muscle of Egyptian buffaloes. The highest IGF2 mRNA level was estimated in the muscle of animals with the AA homozygous genotype as compared to the AC heterozygotes and CC homozygotes. We conclude that A266C SNP at nucleotide number 51 of exon 23 of the IGF2R gene is associated with the ADG during the early stages of life (from birth to 9 mo of age) and this effect is accompanied by, and may be caused by, increased expression levels of the IGF2 gene.
authors.affiliation: Department of Anatomy & Embryology, Faculty of Veterinary Medicine, Kafrelsheikh University, El-Geish Street, Kafrelsheikh, Post Box 33516, Egypt. Electronic address: mohrizk73@yahoo.com.
authors.forename: Mohammed Abu
authors.initials: MA
authors.lastname: El-Magd
authors.affiliation: Department of Animal Wealth Development, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt.
authors.forename: Haitham G
authors.initials: HG
authors.lastname: Abo-Al-Ela
authors.affiliation: Department of Animal Wealth Development, Faculty of Veterinary Medicine, Edfina, Alexandria University, Egypt.
authors.forename: Abeer
authors.initials: A
authors.lastname: El-Nahas
authors.affiliation: Department of Animal Wealth Development, Faculty of Veterinary Medicine, Zagazig University, Egypt.
authors.forename: Ayman A
authors.initials: AA
authors.lastname: Saleh
authors.affiliation: Department of Anatomy & Embryology, Faculty of Veterinary Medicine, Kafrelsheikh University, El-Geish Street, Kafrelsheikh, Post Box 33516, Egypt.
authors.forename: Ali A
authors.initials: AA
authors.lastname: Mansour
mesh_terms: D000818:Animals; D001483:Base Sequence; D002020:Buffaloes / Q000235:genetics* / Q000254:growth & development; D015870:Gene Expression; D005787:Gene Frequency; D056726:Genetic Association Studies; D007335:Insulin-Like Growth Factor II / Q000235:genetics* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017527:Receptor, IGF Type 2 / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D012333:RNA, Messenger; D017527:Receptor, IGF Type 2; D007335:Insulin-Like Growth Factor II
keywords: Average daily gain; Egyptian buffalo; IGF2 gene; IGF2R gene; SNPs; SSCP