title: NAD(+)-linked alcohol dehydrogenase 1 regulates methylglyoxal concentration in Candida albicans.
abstract: We purified a fraction that showed NAD(+)-linked methylglyoxal dehydrogenase activity, directly catalyzing methylglyoxal oxidation to pyruvate, which was significantly increased in glutathione-depleted Candida albicans. It also showed NADH-linked methylglyoxal-reducing activity. The fraction was identified as a NAD(+)-linked alcohol dehydrogenase (ADH1) through mass spectrometric analyses. In ADH1-disruptants of both the wild type and glutathione-depleted cells, the intracellular methylglyoxal concentration increased significantly; defects in growth, differentiation, and virulence were observed; and G2-phase arrest was induced.
authors.affiliation: Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul 151-747, Republic of Korea.
authors.forename: Min-Kyu
authors.initials: MK
authors.lastname: Kwak
authors.affiliation: Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul 151-747, Republic of Korea.
authors.forename: MyungHee
authors.initials: M
authors.lastname: Ku
authors.affiliation: Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul 151-747, Republic of Korea. Electronic address: kangsaou@snu.ac.kr.
authors.forename: Sa-Ouk
authors.initials: SO
authors.lastname: Kang
mesh_terms: D000426:Alcohol Dehydrogenase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002176:Candida albicans / Q000201:enzymology* / Q000254:growth & development / Q000472:pathogenicity; D017124:Conserved Sequence; D005260:Female; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D059565:G2 Phase Cell Cycle Checkpoints; D005978:Glutathione / Q000378:metabolism; D025301:Hyphae / Q000201:enzymology / Q000254:growth & development; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D050296:Microbial Viability; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D011765:Pyruvaldehyde / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014774:Virulence
chemical_list: D005656:Fungal Proteins; D017382:Reactive Oxygen Species; D011765:Pyruvaldehyde; D000426:Alcohol Dehydrogenase; D005978:Glutathione
keywords: Alcohol dehydrogenase 1; Candida albicans; G2-phase arrest; Glutathione; Methylglyoxal