title: Improvement of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for identification of clinically important Candida species.
abstract: BACKGROUND
  Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) is emerging as a rapid and accurate tool for identifying pathogens. MALDI-TOF identification of Candida at the species level remains unsatisfactory because of an incomplete commercial database.
  
  
  METHODS
  Using Bruker Daltonics MALDI Biotyper software, an in-house spectral database library was created with m/z ratios of 2,000 to 20,000 Da from 60 successfully identified clinical isolates of Candida spp. (6 species). It was incorporated into the original database to produce the augmented Bruker-Tianjin database. The augmented database was tested with 347 clinical isolates of 6 species comparing with Bruker database in parallel.
  
  
  RESULTS
  The identification rates at the species level were 91.6% (318/347) using the Bruker database and 99.1% (344/347) for the Bruker-Tianjin database.
  
  
  CONCLUSIONS
  Database expansion and refinement can easily and effectively improve MALDI-TOF MS performance for enhanced clinical pathogen identification.
authors.affiliation: Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Logistics University of Chinese People's Armed Police Forces, Tianjin, China.
authors.forename: Shuangfeng
authors.initials: S
authors.lastname: Yang
authors.affiliation: Clinical Laboratory, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces, Tianjin, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Logistics University of Chinese People's Armed Police Forces, Tianjin, China.
authors.forename: Guoping
authors.initials: G
authors.lastname: Zhao
authors.affiliation: Clinical Laboratory, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces, Tianjin, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.affiliation: Clinical Laboratory, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces, Tianjin, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Logistics University of Chinese People's Armed Police Forces, Tianjin, China.
authors.forename: Jianjia
authors.initials: J
authors.lastname: Yang
authors.affiliation: Clinical Laboratory, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces, Tianjin, China.
authors.forename: Junfang
authors.initials: J
authors.lastname: Wang
authors.affiliation: Clinical Laboratory, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces, Tianjin, China.
authors.forename: Yuntao
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Logistics University of Chinese People's Armed Police Forces, Tianjin, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Hu
authors.affiliation: Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Logistics University of Chinese People's Armed Police Forces, Tianjin, China.
authors.forename: Yaxiao
authors.initials: Y
authors.lastname: Li
authors.affiliation: Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Logistics University of Chinese People's Armed Police Forces, Tianjin, China.
authors.forename: Huabing
authors.initials: H
authors.lastname: Zhao
mesh_terms: D002175:Candida / Q000145:classification / Q000302:isolation & purification*; D016000:Cluster Analysis; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*