title: Momordica cochinchinensis seed extracts suppress migration and invasion of human breast cancer ZR-75-30 cells via down-regulating MMP-2 and MMP-9.
abstract: OBJECTIVE
  Metastases and invasion are the main reasons for oncotherapy failure. Momordica cochinchinensis (Mu Bie Zi in Chinese) had been used for a variety of purposes, and shown anti-cancer action. In this article, we focused on effects on regulation of breast cancer cell ZR-75-30 metastases and invasion by extracts of Momordica cochinchinensis seeds (ESMCs).
  
  
  METHODS
  Effect of ESMCs on ZR-75-30 human breast cancer cells proliferation were evaluated by MTT assay and on invasion and migration by wound-healing and matrigel invasion chamber assays. Expression and protease activity of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, were analyzed by Western blotting and gelatin zymography, respectively.
  
  
  RESULTS
  ESMC revealed strong growth inhibitory effects on ZR-75-30 cells, and effectively inhibited ZR-75-30 cell invasion in a dose-dependent manner. Western blot and gelatin zymography analysis showed that ESMC significantly inhibited the expression and secretion of MMP-2 and MMP-9 in ZR-75-30 cells.
  
  
  CONCLUSIONS
  ESMC has the potential to suppress the migration and invasion of ZR-75-30 cancer cells, and it might prove to of interest in the development of novel inhibitors for breast cancer.
authors.affiliation: School of Medicine, Xi'an Jiaotong University, Xi'an, China E-mail : liuchangxiao@vip.163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Zheng
authors.forename: Yan-Min
authors.initials: YM
authors.lastname: Zhang
authors.forename: Ying-Zhuan
authors.initials: YZ
authors.lastname: Zhan
authors.forename: Chang-Xiao
authors.initials: CX
authors.lastname: Liu
mesh_terms: D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation; D015536:Down-Regulation; D005260:Female; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis*; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis*; D029775:Momordica / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000473:pathology; D010936:Plant Extracts / Q000494:pharmacology*; D012639:Seeds / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects
chemical_list: D010936:Plant Extracts; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9