title: Development and partial characterisation of an antiserum against apolipoprotein B of the short-finned eel, Anguilla australis.
abstract: Despite its key role in transportation of triacylglycerides in blood, the distribution, localisation and molecular weight variants of apolipoprotein B (Apob) in teleost fish have essentially escaped study. To address this, a specific short-finned eel (Anguilla australis) Apob antiserum was produced by an immunised rabbit, purified and partially characterised. Localisation of Apob at both the mRNA (in situ hybridisation) and protein (immunohistochemistry) levels mirrored that of mammals; thus immunostaining was confined to the interstitial spaces of the liver and the vascular core of the intestinal villi. Immunostaining of proteins by Western blotting, followed by high-resolution LC-MS, indicated that peptide sequence coverage of Apob in low-density lipoproteins spanned the full-length protein. We conclude that only full-length Apob is produced by eels and that both liver and intestine are key sites for its synthesis.
authors.affiliation: Department of Zoology, University of Otago, 340 Great King Street, PO Box 56, Dunedin, 9054, New Zealand, erin.damsteegt@otago.ac.nz.
authors.forename: Erin L
authors.initials: EL
authors.lastname: Damsteegt
authors.forename: Hiroko
authors.initials: H
authors.lastname: Mizuta
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Ozaki
authors.forename: Naoshi
authors.initials: N
authors.lastname: Hiramatsu
authors.forename: Takashi
authors.initials: T
authors.lastname: Todo
authors.forename: Akihiko
authors.initials: A
authors.lastname: Hara
authors.forename: Shigeho
authors.initials: S
authors.lastname: Ijiri
authors.forename: Shinji
authors.initials: S
authors.lastname: Adachi
authors.forename: P Mark
authors.initials: PM
authors.lastname: Lokman
mesh_terms: D000595:Amino Acid Sequence; D000811:Anguilla / Q000378:metabolism*; D000818:Animals; D001055:Apolipoproteins B / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D001483:Base Sequence; D005260:Female; D007106:Immune Sera / Q000737:chemistry / Q000276:immunology*; D007413:Intestinal Mucosa / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008969:Molecular Sequence Data
chemical_list: D001055:Apolipoproteins B; D007106:Immune Sera