title: Identification of isoafricanol and its terpene cyclase in Streptomyces violaceusniger using CLSA-NMR.
abstract: The recently developed CLSA-NMR technique that is based on feeding experiments with (13)C-labelled precursors was applied in the identification of isoafricanol as the main volatile terpene emitted by Streptomyces violaceusniger. The isoafricanol synthase of this organism is presented, together with a recent phylogenetic analysis of bacterial terpene cyclases.
authors.affiliation: Institut für Organische Chemie, Hagenring 30, 38106 Braunschweig, Germany. j.dickschat@tu-braunschweig.de.
authors.forename: Ramona
authors.initials: R
authors.lastname: Riclea
authors.forename: Christian A
authors.initials: CA
authors.lastname: Citron
authors.forename: Jan
authors.initials: J
authors.lastname: Rinkel
authors.forename: Jeroen S
authors.initials: JS
authors.lastname: Dickschat
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000302:isolation & purification / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D052617:Solid Phase Microextraction / Q000379:methods*; D013302:Streptomyces / Q000145:classification / Q000378:metabolism*; D013729:Terpenes / Q000032:analysis* / Q000378:metabolism*
chemical_list: D013729:Terpenes; D019883:Alkyl and Aryl Transferases; C037934:terpene carbocyclase