title: Antibacterial activity of large-area monolayer graphene film manipulated by charge transfer.
abstract: Graphene has attracted increasing attention for potential applications in biotechnology due to its excellent electronic property and biocompatibility. Here we use both Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli) to investigate the antibacterial actions of large-area monolayer graphene film on conductor Cu, semiconductor Ge and insulator SiO2. The results show that the graphene films on Cu and Ge can surprisingly inhibit the growth of both bacteria, especially the former. However, the proliferation of both bacteria cannot be significantly restricted by the graphene film on SiO2. The morphology of S. aureus and E. coli on graphene films further confirms that the direct contact of both bacteria with graphene on Cu and Ge can cause membrane damage and destroy membrane integrity, while no evident membrane destruction is induced by graphene on SiO2. From the viewpoint of charge transfer, a plausible mechanism is proposed here to explain this phenomenon. This study may provide new insights for the better understanding of antibacterial actions of graphene film and for the better designing of graphene-based antibiotics or other biomedical applications.
authors.affiliation: 1] State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China [2].
authors.forename: Jinhua
authors.initials: J
authors.lastname: Li
authors.affiliation: 1] State Key Laboratory of Functional Materials for Informatics, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China [2] School of Physical Science and Technology, Lanzhou University, Lanzhou 730000, China [3].
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.
authors.forename: Hongqin
authors.initials: H
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Functional Materials for Informatics, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Functional Materials for Informatics, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Functional Materials for Informatics, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.
authors.forename: Zengfeng
authors.initials: Z
authors.lastname: Di
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.
authors.forename: Xuanyong
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Functional Materials for Informatics, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects* / Q000648:ultrastructure; D003300:Copper / Q000737:chemistry; D004553:Electric Conductivity; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D005857:Germanium / Q000737:chemistry; D006108:Graphite / Q000494:pharmacology*; D050296:Microbial Viability / Q000187:drug effects; D008855:Microscopy, Electron, Scanning; D012822:Silicon Dioxide / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D055672:Static Electricity
chemical_list: D000900:Anti-Bacterial Agents; D005857:Germanium; D012822:Silicon Dioxide; D006108:Graphite; D003300:Copper