title: EB1 enables spindle microtubules to regulate centromeric recruitment of Aurora B.
abstract: The Aurora B kinase coordinates kinetochore-microtubule attachments with spindle checkpoint signaling on each mitotic chromosome. We find that EB1, a microtubule plus end-tracking protein, is required to enrich Aurora B at inner centromeres in a microtubule-dependent manner. This regulates phosphorylation of both kinetochore and chromatin substrates. EB1 regulates the histone phosphorylation marks (histone H2A phospho-Thr120 and histone H3 phospho-Thr3) that localize Aurora B. The chromosomal passenger complex containing Aurora B can be found on a subset of spindle microtubules that exist near prometaphase kinetochores, known as preformed K-fibers (kinetochore fibers). Our data suggest that EB1 enables the spindle microtubules to regulate the phosphorylation of kinetochores through recruitment of the Aurora B kinase.
authors.affiliation: Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA 22908.
authors.forename: Budhaditya
authors.initials: B
authors.lastname: Banerjee
authors.forename: Cortney A
authors.initials: CA
authors.lastname: Kestner
authors.forename: P Todd
authors.initials: PT
authors.lastname: Stukenberg
mesh_terms: D000818:Animals; D064107:Aurora Kinase B / Q000378:metabolism*; D002503:Centromere / Q000201:enzymology*; D002843:Chromatin / Q000378:metabolism; D006367:HeLa Cells; D006657:Histones / Q000378:metabolism; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000502:physiology*; D008870:Microtubules / Q000201:enzymology*; D010766:Phosphorylation; D049468:Prometaphase; D011499:Protein Processing, Post-Translational; D021381:Protein Transport; D008941:Spindle Apparatus / Q000201:enzymology*; D014981:Xenopus
chemical_list: D002843:Chromatin; D006657:Histones; C500985:MAPRE1 protein, human; D008869:Microtubule-Associated Proteins; C576921:AURKB protein, human; D064107:Aurora Kinase B