title: Real-time label-free detection of dividing cells by means of lensfree video-microscopy.
abstract: Quantification of cell proliferation and monitoring its kinetics are essential in fields of research such as developmental biology, oncology, etc. Although several proliferation assays exist, monitoring cell proliferation kinetics remains challenging. We present a novel cell proliferation assay based on real-time monitoring of cell culture inside a standard incubator using a lensfree video-microscope, combined with automated detection of single cell divisions over a population of several thousand cells. Since the method is based on direct visualization of dividing cells, it is label-free, continuous, and not sample destructive. Kinetics of cell proliferation can be monitored from a few hours to several days. We compare our method to a standard assay, the EdU proliferation assay, and as proof of principle, we demonstrate concentration-dependent and time-dependent effect of actinomycin D-a cell proliferation inhibitor.
authors.affiliation: Commissariat à l'énergie atomique et aux énergies alternatives (CEA), LETI, MINATEC, 17 rue des martyrs, Grenoble cedex 9, 38054 France.
authors.forename: Srikanth Vinjimore
authors.initials: SV
authors.lastname: Kesavan
authors.affiliation: Commissariat à l'énergie atomique et aux énergies alternatives (CEA), LETI, MINATEC, 17 rue des martyrs, Grenoble cedex 9, 38054 France.
authors.forename: Fabrice P
authors.initials: FP
authors.lastname: Navarro
authors.affiliation: Commissariat à l'énergie atomique et aux énergies alternatives (CEA), LETI, MINATEC, 17 rue des martyrs, Grenoble cedex 9, 38054 France.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Menneteau
authors.affiliation: Commissariat à l'énergie atomique et aux énergies alternatives (CEA), LETI, MINATEC, 17 rue des martyrs, Grenoble cedex 9, 38054 France.
authors.forename: Frederique
authors.initials: F
authors.lastname: Mittler
authors.affiliation: Plateforme d'imagerie dynamique, Imagopole, Institut Pasteur, Paris, 75015 France.
authors.forename: Brigitte
authors.initials: B
authors.lastname: David-Watine
authors.affiliation: Plateforme d'imagerie dynamique, Imagopole, Institut Pasteur, Paris, 75015 France.
authors.forename: Nelly
authors.initials: N
authors.lastname: Dubrulle
authors.affiliation: Plateforme d'imagerie dynamique, Imagopole, Institut Pasteur, Paris, 75015 France.
authors.forename: Spencer L
authors.initials: SL
authors.lastname: Shorte
authors.affiliation: University of Cergy-Pontoise, UFR Sciences, Cergy 95011 FrancedCMLA, École Normale Supérieure de Cachan, 94230 France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Chalmond
authors.affiliation: Commissariat à l'énergie atomique et aux énergies alternatives (CEA), LETI, MINATEC, 17 rue des martyrs, Grenoble cedex 9, 38054 France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Dinten
authors.affiliation: Commissariat à l'énergie atomique et aux énergies alternatives (CEA), LETI, MINATEC, 17 rue des martyrs, Grenoble cedex 9, 38054 France.
authors.forename: Cedric P
authors.initials: CP
authors.lastname: Allier
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D003584:Cytological Techniques / Q000295:instrumentation* / Q000379:methods*; D007700:Kinetics; D051379:Mice; D018715:Microscopy, Video / Q000295:instrumentation* / Q000379:methods*; D041681:NIH 3T3 Cells