title: Frizzled10 mediates WNT1 and WNT3A signaling in the dorsal spinal cord of the developing chick embryo.
abstract: BACKGROUND
  WNT1 and WNT3A drive a dorsal to ventral gradient of β-catenin-dependent Wnt signaling in the developing spinal cord. However, the identity of the receptors mediating downstream functions remains poorly understood.
  
  
  RESULTS
  In this report, we show that the spatiotemporal expression patterns of FZD10 and WNT1/WNT3A are highly correlated. We further show that in the presence of LRP6, FZD10 promotes WNT1 and WNT3A signaling using an 8xSuperTopFlash reporter assay. Consistent with a functional role for FZD10, we demonstrate that FZD10 is required for proliferation in the spinal cord. Finally, by using an in situ proximity ligation assay, we observe an interaction between FZD10 and WNT1 and WNT3A proteins.
  
  
  CONCLUSIONS
  Together, our results identify FZD10 as a receptor for WNT1 and WNT3A in the developing chick spinal cord.
authors.affiliation: Department of Biology, San Francisco State University, 1600 Holloway Avenue, San Francisco, CA 94132, USA.
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Galli
authors.affiliation: Department of Biology, San Francisco State University, 1600 Holloway Avenue, San Francisco, CA 94132, USA.
authors.forename: Roeben N
authors.initials: RN
authors.lastname: Munji
authors.affiliation: Clemson University, Biological Sciences, Long Hall, Clemson, SC, 29634, USA.
authors.forename: Susan C
authors.initials: SC
authors.lastname: Chapman
authors.affiliation: Department of Biology, San Francisco State University, 1600 Holloway Avenue, San Francisco, CA 94132, USA.
authors.forename: Ann
authors.initials: A
authors.lastname: Easton
authors.affiliation: Department of Biology, San Francisco State University, 1600 Holloway Avenue, San Francisco, CA 94132, USA.
authors.forename: Lydia
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Biology, San Francisco State University, 1600 Holloway Avenue, San Francisco, CA 94132, USA.
authors.forename: Ouma
authors.initials: O
authors.lastname: Onguka
authors.affiliation: Department of Biology, San Francisco State University, 1600 Holloway Avenue, San Francisco, CA 94132, USA.
authors.forename: Joseph S
authors.initials: JS
authors.lastname: Ramahi
authors.affiliation: Department of Biology, San Francisco State University, 1600 Holloway Avenue, San Francisco, CA 94132, USA.
authors.forename: Rowena
authors.initials: R
authors.lastname: Suriben
authors.affiliation: Department of Biology, San Francisco State University, 1600 Holloway Avenue, San Francisco, CA 94132, USA.
authors.forename: Linda A
authors.initials: LA
authors.lastname: Szabo
authors.affiliation: Department of Biology, San Francisco State University, 1600 Holloway Avenue, San Francisco, CA 94132, USA.
authors.forename: Camilla
authors.initials: C
authors.lastname: Teng
authors.affiliation: Department of Biology, San Francisco State University, 1600 Holloway Avenue, San Francisco, CA 94132, USA.
authors.forename: Baouyen
authors.initials: B
authors.lastname: Tran
authors.affiliation: Department of Early Discovery Biochemistry, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA.
authors.forename: Rami N
authors.initials: RN
authors.lastname: Hannoush
authors.affiliation: Department of Biology, San Francisco State University, 1600 Holloway Avenue, San Francisco, CA 94132, USA.
authors.forename: Laura W
authors.initials: LW
authors.lastname: Burrus
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000378:metabolism*; D002642:Chick Embryo; D051157:Frizzled Receptors / Q000378:metabolism*; D013116:Spinal Cord / Q000196:embryology*; D051155:Wnt1 Protein / Q000378:metabolism*; D060509:Wnt3A Protein / Q000378:metabolism*
chemical_list: D030161:Avian Proteins; D051157:Frizzled Receptors; D051155:Wnt1 Protein; D060509:Wnt3A Protein
keywords: FZD10; LRP6; WNT1; WNT3A; chick; palmitate