title: Investigation of the Rho-kinase 2 gene Thr431Asn polymorphism in migraine.
abstract: BACKGROUND
  Migraine has a complex etiology determined by genetic and environmental factors, but the molecular mechanisms and genetics of this disease have not yet been fully clarified.
  
  
  OBJECTIVE
  This case/control study was designed to analyze the genotype distributions and allele frequencies for the Rho-kinase 2 (ROCK2) gene Thr431Asn polymorphism among the migraine patients.
  
  
  METHODS
  A total of 155 migraine patients and 155 healthy age and sex matched controls were included in this study. Genomic deoxyribonucleic acid from migraine patients and controls was analyzed by real-time polymerase chain reaction.
  
  
  RESULTS
  Neither genotype distributions nor the allele frequencies for the Thr431Asn polymorphism showed a significant difference between the groups. In addition, there were no marked differences in genotype and allele frequencies for the migraine without aura and migraine with aura subgroups when compared with control group.
  
  
  CONCLUSIONS
  This is the first study to show that the ROCK2 gene Thr431Asn polymorphism is not a risk factor for the migraine in the Turkish population.
authors.affiliation: Department of Neurology, Emine-Bahaeddin Nakiboglu Medical Faculty, Zirve University, Gaziantep, Turkey.
authors.forename: Samiye Uslu
authors.initials: SU
authors.lastname: Kuzudisli
authors.forename: Mustafa
authors.initials: M
authors.lastname: Yilmaz
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Gül
authors.forename: Seniz
authors.initials: S
authors.lastname: Demiryürek
authors.forename: Remzi
authors.initials: R
authors.lastname: Yigiter
authors.forename: Hakan
authors.initials: H
authors.lastname: Bozkurt
authors.forename: Aylin
authors.initials: A
authors.lastname: Akcali
authors.forename: Münife
authors.initials: M
authors.lastname: Neyal
authors.forename: Cahit
authors.initials: C
authors.lastname: Bagci
authors.forename: Beyhan
authors.initials: B
authors.lastname: Cengiz
authors.forename: Serdar
authors.initials: S
authors.lastname: Oztuzcu
authors.forename: Abdullah T
authors.initials: AT
authors.lastname: Demiryürek
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D008881:Migraine Disorders / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics; D014421:Turkey; D054460:rho-Associated Kinases / Q000235:genetics*
chemical_list: C515852:ROCK2 protein, human; D054460:rho-Associated Kinases