title: In vivo translocation and toxicity of multi-walled carbon nanotubes are regulated by microRNAs.
abstract: We employed an in vivo Caenorhabditis elegans assay system to perform SOLiD sequencing analysis to identify the possible microRNA (miRNA) targets of multi-walled carbon nanotubes (MWCNTs). Bioinformatics analysis on targeted genes for the identified dysregulated miRNAs in MWCNT exposed nematodes demonstrates their involvement in many aspects of biological processes. We used loss-of-function mutants for the identified dysregulated miRNAs to perform toxicity assessment by evaluating functions of primary and secondary targeted organs, and found the miRNA mutants with susceptible or resistant property towards MWCNT toxicity. Both the physiological state of the intestine and defecation behavior were involved in the control of the susceptible or resistant property occurrence for specific miRNA mutants towards MWCNT toxicity. This work provides the molecular basis at the miRNA level for future chemical design to reduce the nanotoxicity of MWCNTs and further elucidation of the related toxicological mechanism.
authors.affiliation: Key Laboratory of Environmental Medicine Engineering in Ministry of Education, Medical School of Southeast University, Nanjing 210009, China. dayongw@seu.edu.cn.
authors.forename: Yunli
authors.initials: Y
authors.lastname: Zhao
authors.forename: Qiuli
authors.initials: Q
authors.lastname: Wu
authors.forename: Yiping
authors.initials: Y
authors.lastname: Li
authors.forename: Abdelli
authors.initials: A
authors.lastname: Nouara
authors.forename: Ruhan
authors.initials: R
authors.lastname: Jia
authors.forename: Dayong
authors.initials: D
authors.lastname: Wang
mesh_terms: D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism*; D003672:Defecation / Q000187:drug effects; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000503:physiopathology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D037742:Nanotubes, Carbon / Q000737:chemistry / Q000633:toxicity*
chemical_list: D035683:MicroRNAs; D037742:Nanotubes, Carbon