title: In vitro Analysis of Antioxidant, Antimicrobial and Antiproliferative Activity of Enteromorpha antenna, Enteromorpha linza and Gracilaria corticata Extracts.
abstract: BACKGROUND
  Seaweeds are taxonomically diverse benthic algae, which are rich in bioactive compounds. These compounds have a potential application in medicine.
  
  
  OBJECTIVE
  The aim of the study was to investigate the bioactive properties of three seaweed samples, Enteromorpha antenna, Enteromorpha linza and Gracilaria corticata were collected from the shoreline of Mahabalipuram, Tamilnadu.
  
  
  METHODS
  Bioactive components were extracted by using various solvents. Antioxidant analysis methods like scavenging activity of nitric oxide, hydrogen peroxide, hydroxyl radicals, free radical scavenging (DPPH), FRAP (ferric reducing ability plasma) ability and reducing power were carried out. MTT assay was employed to study the anticancer activity against cancer cell lines Hep-G2, MCF7 and normal VERO cell lines.
  
  
  RESULTS
  It was found that methanolic extracts elicited higher total phenolic content, higher percentage scavenging activity of nitric oxide, hydrogen peroxide, hydroxyl radicals, free radical scavenging (DPPH), FRAP (ferric reducing ability plasma) ability and reducing power. Different concentrations of crude methanolic extracts of seaweeds showed potential antimicrobial activity by well diffusion method. Crude methanolic extract of G. corticata had significant anticancer activity followed by E. antenna and E. linza on cancer cell lines Hep-G2, MCF7 and normal VERO cell lines by MTT assay.
  
  
  CONCLUSIONS
  The methanolic extracts of seaweeds Enteromorpha antenna, Enteromorpha linza and Gracilaria corticata possess high total phenolic content and shows a good free radical scavenging activity and hence are proven to have better antioxidant activity and they might be good candidates for further investigations in order to develop potential anticancer drugs.
authors.affiliation: Department of Genetic Engineering, School of Bio-engineering, SRM University, Kattankulathur, India.
authors.forename: Manoj Kumar
authors.initials: MK
authors.lastname: Narasimhan
authors.affiliation: Department of Genetic Engineering, School of Bio-engineering, SRM University, Kattankulathur, India.
authors.forename: Shenoy K
authors.initials: SK
authors.lastname: Pavithra
authors.affiliation: Department of Genetic Engineering, School of Bio-engineering, SRM University, Kattankulathur, India.
authors.forename: Vishnupriya
authors.initials: V
authors.lastname: Krishnan
authors.affiliation: Department of Industrial Biotechnology, Government College of Technology, Coimbatore, India.
authors.forename: Muthukumaran
authors.initials: M
authors.lastname: Chandrasekaran
keywords: Antioxidants; Reactive Oxygen Species; Seaweed