title: Effects of titania nanotubes with or without bovine serum albumin loaded on human gingival fibroblasts.
abstract: Modifying the surface of the transmucosal area is a key research area because this process positively affects the three functions of implants: attachment to soft tissue, inhibiting bacterial biofilm adhesion, and the preservation of the crestal bone. To exploit the potential of titania nanotube arrays (TNTs) with or without using bovine serum albumin (BSA) to modify the surface of a dental implant in contact with the transmucosal area, BSA was loaded into TNTs that were fabricated by anodizing Ti sheets; the physical characteristics of these arrays, including their morphology, chemical composition, surface roughness, contact angle, and surface free energy (SFE), were assessed. The effect of Ti surfaces with TNTs or TNTs-BSA on human gingival fibroblasts (HGFs) was determined by analyzing cell morphology, early adhesion, proliferation, type I collagen (COL-1) gene expression, and the extracellular secretion of COL-1. The results indicate that early HGF adhesion and spreading behavior is positively correlated with surface characteristics, including hydrophilicity, SFE, and surface roughness. Additionally, TNT surfaces not only promoted early HGF adhesion, but also promoted COL-1 secretion. BSA-loaded TNT surfaces promoted early HGF adhesion, while suppressing late proliferation and COL-1 secretion. Therefore, TNT-modified smooth surfaces are expected to be applicable for uses involving the transmucosal area. Further study is required to determine whether BSA-loaded TNT surfaces actually affect closed loop formation of connective tissue because BSA coating actions in vivo are very rapid.
authors.affiliation: The First Affiliated Hospital of Jinan University, Guangzhou, People's Republic of China.
authors.forename: Xiangning
authors.initials: X
authors.lastname: Liu
authors.affiliation: Chemistry Science and Technology School, Zhanjiang Normal University, Zhanjiang, People's Republic of China.
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Guangdong Provincial Stomatological Hospital, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Shaobing
authors.initials: S
authors.lastname: Li
authors.affiliation: The First Affiliated Hospital of Jinan University, Guangzhou, People's Republic of China.
authors.forename: Renfa
authors.initials: R
authors.lastname: Lai
authors.affiliation: The First Affiliated Hospital of Jinan University, Guangzhou, People's Republic of China.
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: The First Affiliated Hospital of Jinan University, Guangzhou, People's Republic of China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Guangdong Provincial Stomatological Hospital, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D005881:Gingiva / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry*; D050997:Nanomedicine; D043942:Nanotubes / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000008:administration & dosage*; D013499:Surface Properties; D014025:Titanium / Q000008:administration & dosage*
chemical_list: D024042:Collagen Type I; C009495:titanium dioxide; D012710:Serum Albumin, Bovine; D014025:Titanium
keywords: bovine serum albumin; human gingival fibroblast; modified surface; titania nanotubes; transmucosal area