title: MALDI-TOF mass spectrometry, an efficient technique for in situ detection and characterization of actinomycins.
abstract: An extensive study of actinomycins was performed using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS). Actinomycins represent a well-known family of peptidolactone chromopeptides with potent cytostatic and antibiotic properties. Using five well-characterized streptomycete strains, we introduced MALDI-TOF MS as an efficient technique for rapid in situ detection of actinomycins in surface extracts of cells picked from agar plates. By this procedure, actinomycin complexes can be investigated with high sensitivity and accuracy in a minimum of time. These studies were complemented by mass spectrometric investigation of actinomycins obtained from culture filtrate extracts and purified by high-performance liquid chromatography to detect yet unknown actinomycin species. By feeding experiments, C-demethyl-actinomycins from Streptomyces chrysomallus and Streptomyces parvulus as well as hemi-actinomycins from Streptomyces antibioticus lacking one of the two pentapeptide lactone rings were isolated and characterized as novel variants for structure-activity relationship studies. Structural characterization of the investigated actinomycins was performed by post source decay MALDI-TOF MS. The specific features of the fragmentation patterns of the protonated and cationized forms of selected actinomycins were investigated in detail.
authors.affiliation: Institut für Chemie, Technische Universität Berlin, Müller-Breslau-Straße 10, D 10623, Berlin, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Vater
authors.forename: Ivana
authors.initials: I
authors.lastname: Crnovčić
authors.forename: Siamak
authors.initials: S
authors.lastname: Semsary
authors.forename: Ullrich
authors.initials: U
authors.lastname: Keller
mesh_terms: D003609:Dactinomycin / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013302:Streptomyces / Q000737:chemistry / Q000378:metabolism
chemical_list: D007477:Ions; D003609:Dactinomycin
keywords: MALDI-TOF MS; PSD-MALDI-TOF MS; actinomycins; in situ detection; structural characterization