title: Comparison of protein expression profiles between three Perkinsus spp., protozoan parasites of molluscs, through 2D electrophoresis and mass spectrometry.
abstract: The genus Perkinsus includes protozoan parasites of a wide range of marine molluscs worldwide, some of which have been responsible for heavy mollusc mortalities and dramatic economic losses. This study was performed with the aim of increasing the knowledge of Perkinsus spp. proteome. Proteins extracted from in vitro cultured cells of three species of this genus, P. marinus, P. olseni and P. chesapeaki, were analysed using 2D electrophoresis. Four gels from each species were produced. Qualitative and quantitative comparisons among gels were performed with Proteamweaver software. Cluster analysis grouped the four gels of each Perkinsus sp.; furthermore, P. marinus and P. olseni gels were grouped in a cluster different from P. chesapeaki. Around 2000 spots of each species were considered, from which 213 spots were common to the 3 species; P. chesapeaki and P. marinus shared 310 spots, P. chesapeaki and P. olseni shared 315 spots and P. marinus and P. olseni shared 242 spots. A number of spots were exclusive of each Perkinsus species: 1161 spots were exclusive of P. chesapeaki, 1124 of P. olseni and 895 of P. marinus. A total of 84 spots, including common and species-specific ones, were excised from the gels and analysed using MALDI-TOF and nESI-IT (MS/MS) techniques. Forty-two spots were successfully sequenced, from which 28 were annotated, most of them clustered into electron transport, oxidative stress and detoxification, protein synthesis, carbohydrate metabolism, signal transduction, metabolic process and proteolysis.
authors.affiliation: Centro de Investigacións Mariñas, Consellería do Medio Rural e do Mar, Xunta de Galicia, Aptdo. 13, 36620 Vilanova de Arousa, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Fernández-Boo
authors.affiliation: Department Biochemistry & Molecular Biology, University of Córdoba, Córdoba, Spain; Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC)/Hospital Universitario Reina Sofía/Universidad de Córdoba, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Chicano-Gálvez
authors.affiliation: Department Biochemistry & Molecular Biology, University of Córdoba, Córdoba, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Alhama
authors.affiliation: Department Biochemistry & Molecular Biology, University of Córdoba, Córdoba, Spain.
authors.forename: J L
authors.initials: JL
authors.lastname: Barea
authors.affiliation: Centro de Investigacións Mariñas, Consellería do Medio Rural e do Mar, Xunta de Galicia, Aptdo. 13, 36620 Vilanova de Arousa, Spain. Electronic address: villalba@cimacoron.org.
authors.forename: A
authors.initials: A
authors.lastname: Villalba
authors.affiliation: Centro de Investigacións Mariñas, Consellería do Medio Rural e do Mar, Xunta de Galicia, Aptdo. 13, 36620 Vilanova de Arousa, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Cao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016000:Cluster Analysis; D004141:Dinoflagellida / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008974:Mollusca / Q000469:parasitology*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods
chemical_list: D020543:Proteome
keywords: Electrophoresis 2D; Perkinsus chesapeaki; Perkinsus marinus; Perkinsus olseni; Proteome