title: Enhancing production of alkaline polygalacturonate lyase from Bacillus subtilis by fed-batch fermentation.
abstract: Alkaline polygalacturonate lyase (PGL, EC 4.2.2.2) is an enzyme used in many industries. We developed a fed-batch fermentation process that combines the enzymatic pretreatment of the carbon source with controlling the pH of the fermentative broth to enhance the PGL production from Bacillus subtilis 7-3-3 to decrease the production cost. Maintaining the fermentation broth at pH 6.5 prior to feeding with ammonia and at pH 6.0 after feeding significantly improved PGL activity (743.5 U mL-1) compared with the control (202.5 U mL-1). The average PGL productivity reached 19.6 U mL-1 h-1 after 38 h of fermentation. The crude PGL was suitable for environmentally friendly ramie enzymatic degumming.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Ji-nan City, P.R. China.
authors.forename: Mouyong
authors.initials: M
authors.lastname: Zou
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Ji-nan City, P.R. China.
authors.forename: Fenfen
authors.initials: F
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Ji-nan City, P.R. China.
authors.forename: Xuezhi
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Ji-nan City, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Ji-nan City, P.R. China.
authors.forename: Yinbo
authors.initials: Y
authors.lastname: Qu
mesh_terms: D000645:Ammonium Sulfate / Q000494:pharmacology; D001412:Bacillus subtilis / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D061249:Batch Cell Culture Techniques / Q000379:methods*; D018533:Biomass; D002119:Calcium Carbonate / Q000494:pharmacology; D002244:Carbon / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000378:metabolism; D010368:Pectins / Q000494:pharmacology; D011133:Polysaccharide-Lyases / Q000096:biosynthesis*; D012492:Salts / Q000494:pharmacology; D013997:Time Factors
chemical_list: D012492:Salts; D002244:Carbon; D010368:Pectins; D011133:Polysaccharide-Lyases; C023911:pectate lyase; D002119:Calcium Carbonate; D010100:Oxygen; D000645:Ammonium Sulfate