title: Spatial mapping and quantification of soft and hard protein coronas at silver nanocubes.
abstract: Protein coronas around silver nanocubes were quantified in serum-containing media using localized surface plasmon resonances. Both soft and hard coronas showed exposure-time and concentration-dependent changes in protein surface density with time-dependent hardening. We observed spatially dependent kinetics of the corona-formation at cube edges/corners versus facets at short incubation times, where the polymer stabilization agent delayed corona hardening. The soft corona contained more protein than the hard corona at all time-points (8-fold difference with 10% serum conditions).
authors.affiliation: Interdisciplinary Nanoscience Center (iNANO), Aarhus University , Aarhus, Denmark.
authors.forename: Teodora
authors.initials: T
authors.lastname: Miclăuş
authors.forename: Vladimir E
authors.initials: VE
authors.lastname: Bochenkov
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Ogaki
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: Howard
authors.forename: Duncan S
authors.initials: DS
authors.lastname: Sutherland
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000032:analysis* / Q000378:metabolism; D002417:Cattle; D053768:Metal Nanoparticles / Q000737:chemistry*; D011485:Protein Binding; D012834:Silver / Q000737:chemistry* / Q000378:metabolism; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D001798:Blood Proteins; D012834:Silver