title: Bacterial and methanogenic archaeal communities during the single-stage anaerobic digestion of high-strength food wastewater.
abstract: Single-stage anaerobic digestion (AD) was operated to treat high-strength food wastewater (FWW) derived from food waste recycling facilities at two different organic loading rates (OLRs) of 3.5 (Phase I) and 7 (Phase II) kgCOD/m(3)d. Changes in composition of microbial communities were investigated using quantitative real-time PCR (qPCR) and barcoded-pyrosequencing. At the high FWW loading rate, AD showed efficient performance (i.e., organic matter removal and methane production). Bacterial communities were represented by the phyla Bacteroidetes, Firmicutes, Synergistetes and Actinobacteria. During the entire digestion process, the relative abundance phylum Chloroflexi decreased significantly. The qPCR analysis demonstrated that the methanogenic communities shifted from aceticlastic (Methanosarcinales) to hydrogenotrophic methanogens (Methanobacteriales and Methanomicrobiales) with high increase in the proportion of syntrophic bacterial communities. Canonical correspondence analysis revealed a strong relationship between reactor performance and microbial community shifts.
authors.affiliation: School of Environmental Science and Engineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Pohang 790-784, South Korea.
authors.forename: Hyun Min
authors.initials: HM
authors.lastname: Jang
authors.affiliation: School of Environmental Science and Engineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Pohang 790-784, South Korea.
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Kim
authors.affiliation: School of Environmental Science and Engineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Pohang 790-784, South Korea.
authors.forename: Jeong Hyub
authors.initials: JH
authors.lastname: Ha
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Pohang 790-784, South Korea; Division of Advanced Nuclear Engineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Pohang 790-784, South Korea. Electronic address: jmpark@postech.ac.kr.
authors.forename: Jong Moon
authors.initials: JM
authors.lastname: Park
mesh_terms: D000693:Anaerobiosis; D001105:Archaea / Q000235:genetics / Q000378:metabolism*; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology; D005502:Food; D018628:Gene Dosage; D014644:Genetic Variation; D008697:Methane / Q000096:biosynthesis*; D064307:Microbiota / Q000235:genetics*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D062065:Wastewater / Q000382:microbiology*; D018508:Water Purification / Q000379:methods*
chemical_list: D008697:Methane; D012336:RNA, Ribosomal, 16S; D062065:Wastewater
keywords: Barcoded-pyrosequencing; High-strength food wastewater; Multivariate statistical analysis; Quantitative real-time PCR (qPCR); Single-stage anaerobic digestion