title: C57BL/6N albino/agouti mutant mice as embryo donors for efficient germline transmission of C57BL/6 ES cells.
abstract: We generated C57BL/6NTac mice carrying a tyrosinase loss-of function mutation and a reversion of the nonagouti locus to agouti. This strain has a high superovulation response, allows visual detection of chimeric coat color contribution of C57BL/6 ES-cells and provides a simplified breeding format that generates black G1 offspring of pure inbred C57BL/6 background in one step, providing the ideal host for genetically manipulated C57BL/6 ES cells.
authors.affiliation: Cologne Excellence Cluster on Cellular Stress Responses in Aging-associated Diseases (CECAD), University of Cologne, Cologne, Germany.
authors.forename: Branko
authors.initials: B
authors.lastname: Zevnik
authors.affiliation: TaconicArtemis GmbH, Cologne, Germany.
authors.forename: Nathalie C
authors.initials: NC
authors.lastname: Uyttersprot
authors.affiliation: Taconic, Hudson, New York, United States of America.
authors.forename: Ana V
authors.initials: AV
authors.lastname: Perez
authors.affiliation: Taconic, Hudson, New York, United States of America.
authors.forename: Gerald W M
authors.initials: GW
authors.lastname: Bothe
authors.affiliation: TaconicArtemis GmbH, Cologne, Germany.
authors.forename: Heidrun
authors.initials: H
authors.lastname: Kern
authors.affiliation: TaconicArtemis GmbH, Cologne, Germany.
authors.forename: Gunther
authors.initials: G
authors.lastname: Kauselmann
mesh_terms: D054366:Agouti Signaling Protein / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002678:Chimera / Q000235:genetics; D004624:Embryo Transfer; D053595:Embryonic Stem Cells / Q000502:physiology*; D005260:Female; D056426:Genetic Loci; D006200:Hair Color / Q000235:genetics; D008297:Male; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D014442:Monophenol Monooxygenase / Q000235:genetics; D018626:Retroelements; D013480:Superovulation
chemical_list: D054366:Agouti Signaling Protein; D018626:Retroelements; C515183:a protein, mouse; D014442:Monophenol Monooxygenase