title: Inhibition of vesicular glutamate transporters contributes to attenuate methamphetamine-induced conditioned place preference in rats.
abstract: Accumulating evidence suggests that glutamatergic system plays a crucial role in methamphetamine (METH) addiction. In the glutamatergic transmission, vesicular glutamate transporters (VGLUTs) are responsible for transporting glutamate into synaptic vesicles and affect the glutamate concentrations in the synaptic cleft. It is well documented that VGLUTs play an essential role in pathophysiology of several psychiatric and neurological diseases, however, whether VGLUTs also have a role in addiction caused by psychostimulant drugs is still unknown. The present study was underwent to investigate the effect of inhibition of VGLUTs on METH-induced induce conditioned place preference in rats. Rats were induced to conditioned place preference with METH (0.5, 1.0 and 2.0mg/kg) by intraperitoneal injection. Intracerebroventricular administration of 1.0 or 5.0μg Chicago sky blue 6B (CSB6B), a VGLUTs inhibitor, and 2.5h prior to METH was to observe its effect on METH-induced conditioned place preference in rats. The rats receiving METH showed stronger place preference at the dose of 1.0mg/kg than that of other doses. The intracerebroventricular administration of CSB6B (1.0, 5.0μg) 2.5h prior to the exposure to METH attenuated the acquisition of METH-induced conditioned place preference, while CSB6B itself had no effect on place preference. These results indicate that VGLUTs are involved in the effect of METH-induced conditioned place preference and may be a new target against METH addiction.
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, 27 Tai-Ping Road, Haidian District, Beijing 100850, China.
authors.forename: Zongsheng
authors.initials: Z
authors.lastname: He
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, 27 Tai-Ping Road, Haidian District, Beijing 100850, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, 27 Tai-Ping Road, Haidian District, Beijing 100850, China.
authors.forename: Huajin
authors.initials: H
authors.lastname: Dong
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, 27 Tai-Ping Road, Haidian District, Beijing 100850, China.
authors.forename: Ruibin
authors.initials: R
authors.lastname: Su
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, 27 Tai-Ping Road, Haidian District, Beijing 100850, China.
authors.forename: Zehui
authors.initials: Z
authors.lastname: Gong
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, 27 Tai-Ping Road, Haidian District, Beijing 100850, China. Electronic address: yanlingdi@hotmail.com.
authors.forename: Lingdi
authors.initials: L
authors.lastname: Yan
mesh_terms: D019969:Amphetamine-Related Disorders / Q000188:drug therapy / Q000378:metabolism; D000818:Animals; D002491:Central Nervous System Agents / Q000494:pharmacology; D000697:Central Nervous System Stimulants / Q000494:pharmacology*; D003213:Conditioning, Psychological / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D057967:Infusions, Intraventricular; D007274:Injections, Intraperitoneal; D008297:Male; D008694:Methamphetamine / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D013028:Space Perception / Q000187:drug effects* / Q000502:physiology; D013997:Time Factors; D014343:Trypan Blue / Q000494:pharmacology; D050496:Vesicular Glutamate Transport Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D002491:Central Nervous System Agents; D000697:Central Nervous System Stimulants; D050496:Vesicular Glutamate Transport Proteins; C009000:pontamine sky blue; D008694:Methamphetamine; D014343:Trypan Blue
keywords: Chicago sky blue 6B.; Conditioned place preference; Methamphetamine; Vesicular glutamate transporters