title: Electrodeposition of nanostructured bioactive hydroxyapatite-heparin composite coatings on titanium for dental implant applications.
abstract: In this paper we describe the one-pot fabrication of hydroxyapatite (HA)-heparin composites by electrodeposition onto Ti substrates and their characterisation in terms of structure, morphology, heparin content and bioactivity. HA coatings are well known and widely applied osteointegration enhancers, but post-implant healing rate in dental applications is still suboptimal: e.g. coagulation control plays a key role and the incorporation of an anticoagulant is considered a highly desirable option. In this study, we have developed an improved, simple and robust growth procedure for single-phase, pure HA-heparin films of thickness 1/3 μm. HA-heparin, forming nanowires, has the ideal morphology for bone mineralisation. Staining assays revealed homogeneous incorporation of sizable amounts of heparin in the composite films. The bioactivities of the HA and HA-heparin coatings on Ti were compared by HeLa cell proliferation/viability tests and found to be enhanced by the presence of the anticoagulant.
authors.affiliation: Dipartimento di Ingegneria dell'Innovazione, Università del Salento, via Monteroni, 73100, Lecce, Italy, benedetto.bozzini@unisalento.it.
authors.forename: Benedetto
authors.initials: B
authors.lastname: Bozzini
authors.forename: Amilcare
authors.initials: A
authors.lastname: Barca
authors.forename: Francesco
authors.initials: F
authors.lastname: Bogani
authors.forename: Marco
authors.initials: M
authors.lastname: Boniardi
authors.forename: Paolo
authors.initials: P
authors.lastname: Carlino
authors.forename: Claudio
authors.initials: C
authors.lastname: Mele
authors.forename: Tiziano
authors.initials: T
authors.lastname: Verri
authors.forename: Alessandro
authors.initials: A
authors.lastname: Romano
mesh_terms: D000925:Anticoagulants / Q000008:administration & dosage / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D015921:Dental Implants; D003764:Dental Materials / Q000138:chemical synthesis*; D017886:Durapatite; D004595:Electroplating / Q000379:methods; D006367:HeLa Cells; D006493:Heparin / Q000008:administration & dosage*; D006801:Humans; D008422:Materials Testing; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology*; D010316:Particle Size; D011474:Prosthesis Design; D014025:Titanium / Q000737:chemistry*
chemical_list: D000925:Anticoagulants; D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D003764:Dental Materials; D053769:Nanocapsules; D006493:Heparin; D017886:Durapatite; D014025:Titanium