title: Design of clone-specific probes from genome sequences for rapid PCR-typing of outbreak pathogens.
abstract: The genome sequence of one OXA-48-producing Klebsiella pneumoniae belonging to sequence type (ST) 405, and three belonging to ST11, were used to design and test ST-specific PCR assays for typing OXA-48-producing K. pneumoniae. The approach proved to be useful for in-house development of rapid PCR typing assays for local outbreak surveillance.
authors.affiliation: Servicio de Microbiología, Hospital Universitario La Paz, IdiPAZ, Madrid, Spain.
authors.forename: E
authors.initials: E
authors.lastname: López-Camacho
authors.forename: Z
authors.initials: Z
authors.lastname: Rentero
authors.forename: G
authors.initials: G
authors.lastname: Ruiz-Carrascoso
authors.forename: J-J
authors.initials: JJ
authors.lastname: Wesselink
authors.forename: M
authors.initials: M
authors.lastname: Pérez-Vázquez
authors.forename: S
authors.initials: S
authors.lastname: Lusa-Bernal
authors.forename: P
authors.initials: P
authors.lastname: Gómez-Puertas
authors.forename: R A
authors.initials: RA
authors.lastname: Kingsley
authors.forename: P
authors.initials: P
authors.lastname: Gómez-Sánchez
authors.forename: J
authors.initials: J
authors.lastname: Campos
authors.forename: J
authors.initials: J
authors.lastname: Oteo
authors.forename: J
authors.initials: J
authors.lastname: Mingorance
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D004196:Disease Outbreaks; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology* / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*
keywords: Bacterial genomes; Klebsiella pneumoniae; OXA-48; PCR typing; hospital infection