title: Sensitive monoclonal antibody-based immunoassays for kresoxim-methyl analysis in QuEChERS-based food extracts.
abstract: Kresoxim-methyl is nowadays widely used to combat a diversity of common diseases affecting high-value crops. In this article, we report the development and characterization of two novel immunoassays for the analysis of this pioneer strobilurin fungicide, and for the first time, a validation study with food samples was performed. A direct and an indirect competitive immunoassay based on a new anti-kresoxim-methyl monoclonal antibody were developed for sensitive and specific chemical analysis. Optimized assays showed limits of detection of 0.1 μg/L. Fruit and vegetable samples were extracted with acetonitrile by the QuEChERS procedure and analyzed by the developed immunoassays after a simple dilution in buffer, affording limits of quantification below US and European maximum residue limits. Immunochemical results of samples from kresoxim-methyl-sprayed strawberry fields demonstrated good statistical agreement with gas chromatography coupled to mass spectrometry as reference technique.
authors.affiliation: Department of Biotechnology, Institute of Agrochemistry and Food Technology, Consejo Superior de Investigaciones Científicas (IATA-CSIC) , Agustí Escardino 7, 46980 Paterna, València, Spain.
authors.forename: Josep V
authors.initials: JV
authors.lastname: Mercader
authors.forename: Rosario
authors.initials: R
authors.lastname: López-Moreno
authors.forename: Francesc A
authors.initials: FA
authors.lastname: Esteve-Turrillas
authors.forename: Consuelo
authors.initials: C
authors.lastname: Agulló
authors.forename: Antonio
authors.initials: A
authors.lastname: Abad-Somovilla
authors.forename: Antonio
authors.initials: A
authors.lastname: Abad-Fuentes
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis; D018553:Cucumis sativus / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D031985:Fragaria / Q000737:chemistry; D005638:Fruit / Q000737:chemistry; D005659:Fungicides, Industrial / Q000032:analysis* / Q000302:isolation & purification; D018551:Solanum lycopersicum / Q000737:chemistry*; D008689:Methacrylates / Q000032:analysis / Q000302:isolation & purification; D010573:Pesticide Residues / Q000032:analysis* / Q000302:isolation & purification; D010648:Phenylacetates / Q000032:analysis* / Q000302:isolation & purification; D052616:Solid Phase Extraction / Q000379:methods; D000073739:Strobilurins
chemical_list: D000911:Antibodies, Monoclonal; D005659:Fungicides, Industrial; D008689:Methacrylates; D010573:Pesticide Residues; D010648:Phenylacetates; D000073739:Strobilurins; C469328:kresoxim-methyl