title: Association of HLA-DRB1 alleles and anti-neutrophil cytoplasmic antibodies in Han and Uyghur patients with ulcerative colitis in China.
abstract: OBJECTIVE
  We aimed to study the association between HLA-DRB1 alleles and anti-neutrophil cytoplasmic antibodies (ANCA) among Uyghur and Han patients with ulcerative colitis (UC) in China.
  
  
  METHODS
  Altogether 160 UC patients and 466 healthy controls of Uyghur and Han groups residing in the Xinjiang Uyghur Autonomous Region of China were included. HLA-DRB1 variants were identified from genomic DNA using polymerase chain reaction and gene sequencing. Serum ANCA were determined by indirect immunofluorescence assay.
  
  
  RESULTS
  Among the Uyghur populations, the HLA-DRB1*08 gene frequency was lower in the UC patients than in the control group (P = 0.012, OR 0.12, 95% CI 0.02-0.91); however, that of HLA-DRB1*13 was much higher in the UC patients than in the controls (P = 0.001, OR 4.32, 95% CI 1.92-9.74). In Han patients with UC, there was no significant difference in HLA-DRB1 frequency between UC patients and healthy controls. The positive rate of ANCA in Uyghur patients with UC was significantly higher than in Han UC patients (P = 0.026), and ANCA positivity was associated with an increased frequency of HLA-DRB1*13 in Uyghur UC patients, but no such difference was observed in the Han patients.
  
  
  CONCLUSIONS
  Genetic polymorphisms of the HLA-DRB1*08 and *13 may contribute to the clinical heterogeneity of UC between Uyghur and Han UC patients in China. In Uyghur UC patients, HLA-DRB1*13 may be correlated with ANCA positivity.
authors.affiliation: Department of Gastroenterology, People's Hospital of Xinjiang Uyghur Autonomous Region, Urumqi, Xinjiang Uyghur Autonomous Region, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Ayinuer
authors.initials: A
authors.lastname: Aheman
authors.forename: Jia Jie
authors.initials: JJ
authors.lastname: Lu
authors.forename: Mireayi
authors.initials: M
authors.lastname: Abuduhadeer
authors.forename: Yue Xian
authors.initials: YX
authors.lastname: Li
authors.forename: Aihemaijiang
authors.initials: A
authors.lastname: Kuerbanjiang
mesh_terms: D000328:Adult; D000483:Alleles; D019268:Antibodies, Antineutrophil Cytoplasmic / Q000097:blood*; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D003093:Colitis, Ulcerative / Q000208:ethnology / Q000235:genetics* / Q000276:immunology; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D059811:HLA-DRB1 Chains / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic
chemical_list: D019268:Antibodies, Antineutrophil Cytoplasmic; D059811:HLA-DRB1 Chains; C506473:HLA-DRB1*13 antigen
keywords: HLA-DRB1 chains; anti-neutrophil cytoplasmic antibody; ethnic group; inflammatory bowel disease; ulcerative colitis