title: Specific forms of BAFF favor BAFF receptor-mediated epithelial cell survival.
abstract: Although B cell activating factor (BAFF) and its receptor BR3 are produced and expressed by many cells, their role has been restricted to the lymphocyte lineage. Using various techniques (RT-PCR, indirect immunofluorescence, flow cytometry analysis), we observed the expression of BR3 and the production of BAFF by the human salivary gland cell line, by epithelial cells from biopsies of Sjögren's syndrome patients and their controls, but also by salivary gland epithelial cells in culture. To decipher the role of BAFF and BR3 on epithelial cells, BAFF and BR3 were neutralized by blocking antibodies or RNA specific inhibitor (siBR3) and epithelial cell survival was analyzed. Blocking BR3 promotes epithelial cell apoptosis in vitro. This apoptosis resulted in the nuclear translocation of PKCδ. BAFF neutralization by various anti-BAFF antibodies leads to different effects depending on the antibody used suggesting that only some forms of BAFF are required for epithelial cell survival. Our study demonstrates that BR3 is involved in the survival of cultured epithelial cells due to an autocrine effect of BAFF. It also suggests that epithelial cells produce different forms of BAFF and that only some of them are responsible for this effect.
authors.affiliation: EA2216 "Immunology and Pathology", LabEx IGO, SFR ScInBioS, European University of Brittany and Brest University, Brest, France.
authors.forename: Ayan
authors.initials: A
authors.lastname: Lahiri
authors.affiliation: EA2216 "Immunology and Pathology", LabEx IGO, SFR ScInBioS, European University of Brittany and Brest University, Brest, France.
authors.forename: Marie-Michèle
authors.initials: MM
authors.lastname: Varin
authors.affiliation: EA2216 "Immunology and Pathology", LabEx IGO, SFR ScInBioS, European University of Brittany and Brest University, Brest, France.
authors.forename: Laëtitia
authors.initials: L
authors.lastname: Le Pottier
authors.affiliation: EA2216 "Immunology and Pathology", LabEx IGO, SFR ScInBioS, European University of Brittany and Brest University, Brest, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Pochard
authors.affiliation: EA2216 "Immunology and Pathology", LabEx IGO, SFR ScInBioS, European University of Brittany and Brest University, Brest, France.
authors.forename: Boutahar
authors.initials: B
authors.lastname: Bendaoud
authors.affiliation: EA2216 "Immunology and Pathology", LabEx IGO, SFR ScInBioS, European University of Brittany and Brest University, Brest, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Youinou
authors.affiliation: EA2216 "Immunology and Pathology", LabEx IGO, SFR ScInBioS, European University of Brittany and Brest University, Brest, France. Electronic address: pers@univ-brest.fr.
authors.forename: Jacques-Olivier
authors.initials: JO
authors.lastname: Pers
mesh_terms: D000328:Adult; D000368:Aged; D019138:Antibodies, Blocking / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D057134:Antibodies, Neutralizing / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D053264:B-Cell Activating Factor / Q000235:genetics / Q000378:metabolism*; D053265:B-Cell Activation Factor Receptor / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D001706:Biopsy; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D004847:Epithelial Cells / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D008297:Male; D008875:Middle Aged; D012469:Salivary Glands / Q000473:pathology; D012859:Sjogren's Syndrome / Q000235:genetics / Q000276:immunology / Q000378:metabolism
chemical_list: D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D053264:B-Cell Activating Factor; D053265:B-Cell Activation Factor Receptor; C505719:TNFSF13B protein, human
keywords: BAFF; BR3; Epithelial cells; Protein kinase C δ; Salivary glands; Sjögren's syndrome