title: Knockdown expression of eukaryotic initiation factor 5 C-terminal domain containing protein extends lifespan in Drosophila melanogaster.
abstract: Inhibition of translation by mutations of a growing number of genes involved in protein synthesis could extend healthy lifespan in yeast, worm, fly and mouse as well. These genes vary from translation initiation factors to structural components of ribosomes and ribosomal RNA processing factors. ECP is a novel ribosome associated protein. Previous data supports the involvement of this gene in long term memory formation and exon guidance in Drosophila probably through its still unconfirmed functions in protein synthesis. However, the exact molecular function of ECP is still largely unknown. Our findings here show that fly lifespan could be significantly extended in ECP RNAi flies. Meanwhile, the locomotion ability of elder ECP RNAi flies was also improved remarkably. Further studies revealed an increase of mitochondria Complex IV activity in these ECP RNAi flies. A decrease of AKT and S6K phosphorylation level in contrast to an increase of AMPK phosphorylation level could also be detected in these flies. Together, these findings support a positive effect of ECP on longevity and delaying age-related impairment in locomotor behavior probably through activation of AMPK and enhancement of mitochondrial function via insulin/IGF-1 and TOR pathway.
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical School, Southeast University, Nanjing 210009, China. Electronic address: daoyongwang@seu.edu.cn.
authors.forename: Daoyong
authors.initials: D
authors.lastname: Wang
authors.affiliation: Institute of Life Sciences, The Key Laboratory of Developmental Genes and Human Disease, Southeast University, Nanjing 210096, China. Electronic address: cuiyuanping16@126.com.
authors.forename: Yuanping
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Institute of Life Sciences, The Key Laboratory of Developmental Genes and Human Disease, Southeast University, Nanjing 210096, China. Electronic address: nye806468948@126.com.
authors.forename: Zhixia
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Institute of Life Sciences, The Key Laboratory of Developmental Genes and Human Disease, Southeast University, Nanjing 210096, China. Electronic address: wei.xie@seu.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Xie
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000502:physiology*; D040061:Eukaryotic Initiation Factor-5; D055785:Gene Knockdown Techniques / Q000379:methods*; D008136:Longevity / Q000502:physiology*; D010448:Peptide Initiation Factors / Q000235:genetics*
chemical_list: D029721:Drosophila Proteins; D040061:Eukaryotic Initiation Factor-5; C520428:Krasavietz protein, Drosophila; D010448:Peptide Initiation Factors
keywords: AKT; AMPK; Drosophila melanogaster; ECP/KRA; Longevity; S6K