title: Goose Hemorrhagic polyomavirus detection in geese using real-time PCR assay.
abstract: Goose hemorrhagic polyomavirus (GHPV) is the viral agent of hemorrhagic nephritis enteritis of geese (HNEG), a lethal disease of goslings. Although death is the most common outcome, geese that recover from HNEG are persistently infected. Here, we present the development of real-time SYBR Green real-time PCR targeted to GHPV and its use to assess the prevalence of GHPV infection in French geese flocks. When compared with classical end-point PCR, real-time PCR revealed a much better sensitivity and equivalent specificity. Real-time PCR could, therefore, be considered a gold standard for the detection of GHPV. Results of field investigations evidenced a very high prevalence of GHPV infections in French geese, largely associated with healthy carriage.
authors.affiliation: Université de Toulouse, Institut National Polytechnique, Ecole Nationale Vétérinaire de Toulouse, 23, chemin des Capelles, F-31076 Toulouse, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Leon
authors.affiliation: Université de Toulouse, Institut National Polytechnique, Ecole Nationale Vétérinaire de Toulouse, 23, chemin des Capelles, F-31076 Toulouse, France.
authors.forename: Léni
authors.initials: L
authors.lastname: Corrand
authors.affiliation: Université de Toulouse, Institut National Polytechnique, Ecole Nationale Vétérinaire de Toulouse, 23, chemin des Capelles, F-31076 Toulouse, France.
authors.forename: Tran Ngoc
authors.initials: TN
authors.lastname: Bich
authors.affiliation: Université de Toulouse, Institut National Polytechnique, Ecole Nationale Vétérinaire de Toulouse, 23, chemin des Capelles, F-31076 Toulouse, France.
authors.forename: Odile
authors.initials: O
authors.lastname: Le Minor
authors.affiliation: Université de Toulouse, Institut National Polytechnique, Ecole Nationale Vétérinaire de Toulouse, 23, chemin des Capelles, F-31076 Toulouse, France.
authors.forename: Mylène
authors.initials: M
authors.lastname: Lemaire
authors.affiliation: Université de Toulouse, Institut National Polytechnique, Ecole Nationale Vétérinaire de Toulouse, 23, chemin des Capelles, F-31076 Toulouse, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Guérin
mesh_terms: D000818:Animals; D004279:DNA, Viral / Q000097:blood / Q000235:genetics / Q000378:metabolism; D004751:Enteritis / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary / Q000821:virology; D005602:France; D005777:Geese; D009393:Nephritis / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary / Q000821:virology; D011120:Polyomavirus / Q000235:genetics* / Q000378:metabolism; D027601:Polyomavirus Infections / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D011201:Poultry Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods* / Q000662:veterinary
chemical_list: D004279:DNA, Viral