title: REIL proteins of Arabidopsis thaliana interact in yeast-2-hybrid assays with homologs of the yeast Rlp24, Rpl24A, Rlp24B, Arx1, and Jjj1 proteins.
abstract: The REIL1 and REIL2 proteins of Arabidopsis thaliana are evolutionarily conserved homologs of the cytosolic 60S ribosomal maturation factors Rei1 and its paralog Reh1 of Saccharomyces cerevisiae. We previously demonstrated that the REIL proteins like the yeast homologs are required for the growth of both organisms at suboptimal temperatures. In addition, the cold sensitivity of the yeast Δrei1 mutant was almost fully rescued by heterologous expression of the REIL1 protein. These phenomena and conservation of co-expressed genes linked the function of REIL proteins to the maturation of the eukaryotic ribosome in A. thaliana. Here we demonstrate that REIL proteins interact in yeast-2-hybrid assays with A. thaliana homologs of the yeast proteins, Rlp24, Rpl24A, Rlp24B, Arx1, and Jjj1. These proteins take part in the cytosolic 60S ribosomal maturation process within yeast and physically interact with Rei1. Our study does not provide proof but is consistent with a conserved role of the A. thaliana REIL proteins in ribosomal maturation and demonstrates the potential of future investigations that aim to unravel the protein interactions of REIL proteins in planta.
authors.affiliation: Max-Planck-Institute of Molecular Plant Physiology; Potsdam-Golm, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Schmidt
authors.affiliation: Max-Planck-Institute of Molecular Plant Physiology; Potsdam-Golm, Germany.
authors.forename: Frederik
authors.initials: F
authors.lastname: Dethloff
authors.affiliation: Max-Planck-Institute of Molecular Plant Physiology; Potsdam-Golm, Germany.
authors.forename: Olga
authors.initials: O
authors.lastname: Beine-Golovchuk
authors.affiliation: Max-Planck-Institute of Molecular Plant Physiology; Potsdam-Golm, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Kopka
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D054683:Ribosome Subunits, Large, Eukaryotic / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D029681:Arabidopsis Proteins; C000590491:REIL1 protein, Arabidopsis; C000590492:REIL2 protein, Arabidopsis; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors
keywords: Cold sensitivity; REIL proteins; cytosolic maturation of the eukaryotic ribosome; leaf growth; protein–protein interaction; yeast-2-hybrid assay