title: Ex vivo localization and permeation of cisplatin from novel topical formulations through excised pig, goat, and mice skin and in vitro characterization for effective management of skin-cited malignancies.
abstract: BACKGROUND
  It would be advantageous to administer cisplatin topically for treatment of cutaneous malignancies.
  
  
  OBJECTIVE
  Present work focuses on ex vivo and in vitro characterization of proultraflexible topical formulations.
  
  
  METHODS
  Permeation of cisplatin through the excised pig, goat, and mice skin was quantitatively determined.
  
  
  RESULTS
  Data indicate that protransfersome carbopol gel (pcg) formulation clearly delayed drug permeation through skin. Permeation of cisplatin from protransfersome system (ps) formulation was enhanced by approximately 1.5 fold compared with pcg for pig and goat skin.
  
  
  CONCLUSIONS
  Localization of drug from pcg was higher and showed less permeation.
  
  
  CONCLUSIONS
  Cisplatin-loaded pcg formulation is better to treat cutaneous malignancies.
authors.affiliation: a School of Pharmaceutical Sciences, Rajiv Gandhi Technical University , Gandhi Nagar, Bhopal , India.
authors.forename: Vandana
authors.initials: V
authors.lastname: Gupta
authors.affiliation: a School of Pharmaceutical Sciences, Rajiv Gandhi Technical University , Gandhi Nagar, Bhopal , India.
authors.forename: Piyush
authors.initials: P
authors.lastname: Trivedi
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000279:Administration, Cutaneous; D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism / Q000494:pharmacology*; D001692:Biological Transport; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D002945:Cisplatin / Q000378:metabolism / Q000494:pharmacology*; D016503:Drug Delivery Systems / Q000379:methods*; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology; D005782:Gels; D006041:Goats; D006801:Humans; D051379:Mice; D010539:Permeability; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D012869:Skin Absorption; D013552:Swine
chemical_list: D000180:Acrylic Resins; D000970:Antineoplastic Agents; D005782:Gels; C006912:carboxypolymethylene; D002945:Cisplatin
keywords: A-431 cell line; cutaneous squamous cell carcinoma; cytotoxic activity; fluorescence microscopy; local accumulation efficiency; proultraflexible topical formulations