title: A new experimental model of cigarette smoke-induced emphysema in Wistar rats.
abstract: OBJECTIVE
  To describe a new murine model of cigarette smoke-induced emphysema.
  
  
  METHODS
  Twenty-four male Wistar rats were divided into two groups: the cigarette smoke group, comprising 12 rats exposed to smoke from 12 commercial filter cigarettes three times a day (a total of 36 cigarettes per day) every day for 30 weeks; and the control group, comprising 12 rats exposed to room air three times a day every day for 30 weeks. Lung function was assessed by mechanical ventilation, and emphysema was morphometrically assessed by measurement of the mean linear intercept (Lm).
  
  
  RESULTS
  The mean weight gain was significantly (approximately ten times) lower in the cigarette smoke group than in the control group. The Lm was 25.0% higher in the cigarette smoke group. There was a trend toward worsening of lung function parameters in the cigarette smoke group.
  
  
  CONCLUSIONS
  The new murine model of cigarette smoke-induced emphysema and the methodology employed in the present study are effective and reproducible, representing a promising and economically viable option for use in studies investigating the pathophysiology of and therapeutic approaches to COPD.
authors.affiliation: University of São Paulo, São Paulo, Brazil, Graduate Student. University of São Paulo, São Paulo, Brazil.
authors.forename: Rodrigo de las Heras
authors.initials: Rde L
authors.lastname: Kozma
authors.affiliation: Júlio de Mesquita Filho São Paulo State University, Assis School of Sciences and Languages, Assis, Brazil, Undergraduate Student. Júlio de Mesquita Filho São Paulo State University at Assis School of Sciences and Languages, Assis, Brazil.
authors.forename: Edson Marcelino
authors.initials: EM
authors.lastname: Alves
authors.affiliation: University of São Paulo, São Paulo, Brazil, Graduate Student. University of São Paulo, São Paulo, Brazil.
authors.forename: Valter Abraão
authors.initials: VA
authors.lastname: Barbosa-de-Oliveira
authors.affiliation: Júlio de Mesquita Filho São Paulo State University, Botucatu, Brazil, Researcher. Júlio de Mesquita Filho São Paulo State University, Botucatu, Brazil.
authors.forename: Fernanda Degobbi Tenorio Quirino dos Santos
authors.initials: FD
authors.lastname: Lopes
authors.affiliation: Faculdades Integradas Padre Albino, Catanduva, Brazil, Undergraduate Student. Faculdades Integradas Padre Albino, Catanduva, Brazil.
authors.forename: Renan Cenize
authors.initials: RC
authors.lastname: Guardia
authors.affiliation: Faculdades Integradas Padre Albino, Catanduva, Brazil, Undergraduate Student. Faculdades Integradas Padre Albino, Catanduva, Brazil.
authors.forename: Henrique Vivi
authors.initials: HV
authors.lastname: Buzo
authors.affiliation: University of São Paulo, São Paulo, Brazil, Graduate Student. University of São Paulo, São Paulo, Brazil.
authors.forename: Carolina Arruda de
authors.initials: CA
authors.lastname: Faria
authors.affiliation: Júlio de Mesquita Filho São Paulo State University, Assis School of Sciences and Languages, Assis, Brazil, Undergraduate Student. Júlio de Mesquita Filho São Paulo State University at Assis School of Sciences and Languages, Assis, Brazil.
authors.forename: Camila
authors.initials: C
authors.lastname: Yamashita
authors.affiliation: Faculdades Integradas Padre Albino, Catanduva, Brazil, Associate Professor. Faculdades Integradas Padre Albino, Catanduva, Brazil.
authors.forename: Manzelio
authors.initials: M
authors.lastname: Cavazzana  Júnior
authors.affiliation: Júlio de Mesquita Filho São Paulo State University, Assis School of Sciences and Languages, Assis, Brazil, Associate Professor. Júlio de Mesquita Filho São Paulo State University at Assis School of Sciences and Languages, Assis, Brazil.
authors.forename: Fernando
authors.initials: F
authors.lastname: Frei
authors.affiliation: Faculdades Integradas Padre Albino, Catanduva, Brazil, Associate Professor. Faculdades Integradas Padre Albino, Catanduva, Brazil.
authors.forename: Maria José de Oliveira
authors.initials: MJ
authors.lastname: Ribeiro-Paes
authors.affiliation: Júlio de Mesquita Filho São Paulo State University, Assis School of Sciences and Languages, Assis, Brazil, Assistant Professor. Júlio de Mesquita Filho São Paulo State University at Assis School of Sciences and Languages, Assis, Brazil.
authors.forename: João Tadeu
authors.initials: JT
authors.lastname: Ribeiro-Paes
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D008297:Male; D011656:Pulmonary Emphysema / Q000209:etiology*; D051381:Rats; D017208:Rats, Wistar; D012121:Respiration, Artificial; D013997:Time Factors; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*
chemical_list: D014028:Tobacco Smoke Pollution