title: (1)H, (15)N and (13)C resonance assignments of the conserved region in the middle domain of S. pombe Sin1 protein.
abstract: SAPK-interacting protein 1 (Sin1) is an important component of the target of rapamycin (TOR) complex 2 (TORC2). TOR is a serine/threonine-specific protein kinase and forms functionally distinct protein complexes referred to as TORC1 and TORC2. TORC2, conserved from yeast to humans, phosphorylates AGC-family protein kinases and has many cellular functions including the regulation of actin cytoskeleton. The Sin1 subunit of TORC2 is required for the binding of TORC2 to substrates, and the conserved region in the middle (CRIM) domain of Sin1 is important in the substrate recognition of TORC2. Here, we report on the (1)H, (13)C and (15)N resonance assignments of fission yeast Schizosaccharomyces pombe Sin1 (amino acids 247-400) (Sin1CRIM), which possesses the CRIM domain. These data contribute toward the structure determination of Sin1CRIM and an understanding of the interactions of Sin1CRIM with substrates of TORC2.
authors.affiliation: Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka, 565-0871, Japan.
authors.forename: Saori
authors.initials: S
authors.lastname: Kataoka
authors.forename: Kyoko
authors.initials: K
authors.lastname: Furuita
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Hattori
authors.forename: Naohiro
authors.initials: N
authors.lastname: Kobayashi
authors.forename: Takahisa
authors.initials: T
authors.lastname: Ikegami
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Shiozaki
authors.forename: Toshimichi
authors.initials: T
authors.lastname: Fujiwara
authors.forename: Chojiro
authors.initials: C
authors.lastname: Kojima
mesh_terms: D002352:Carrier Proteins / Q000737:chemistry*; D017124:Conserved Sequence; D019906:Nuclear Magnetic Resonance, Biomolecular; D017434:Protein Structure, Tertiary; D012568:Schizosaccharomyces; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry*
chemical_list: D002352:Carrier Proteins; D029702:Schizosaccharomyces pombe Proteins; C000602849:Sin1 protein, S pombe