title: Quinovic acid glycosides purified fraction from Uncaria tomentosa induces cell death by apoptosis in the T24 human bladder cancer cell line.
abstract: Bladder cancer is the second most prevalent malignancy in the genitourinary tract and remains a therapeutic challenge. In the search for new treatments, researchers have attempted to find compounds with low toxicity. With this goal in mind, Uncaria tomentosa is noteworthy because the bark and root of this species are widely used in traditional medicine and in adjuvant therapy for the treatment of numerous diseases. The objective of this study was to investigate the antitumor effect of one purified bioactive fraction of U.tomentosa bark on cell proliferation in two human bladder cancer cell lines, T24 and RT4. Quinovic acid glycosides purified fraction (QAPF) of U.tomentosa decreased the growth and viability of both T24 and RT4 cell lines. In T24 cells, QAPF induced apoptosis by activating caspase-3 and NF-κB. Further study showed that this fraction does not induce cell cycle arrest and does not alter PTEN and ERK levels. In conclusion, we demonstrated that QAPF of U.tomentosa has a potent inhibitory effect on the growth of human bladder cancer cell lines by inducing apoptosis through modulation of NF-κB, and we suggest that QAPF may become a potential therapeutic agent for the prevention and/or treatment of this cancer.
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos, 2600 - anexo - Santana, CEP 90035-003, Porto Alegre, RS, Brazil.
authors.forename: Fabrícia
authors.initials: F
authors.lastname: Dietrich
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Avenida Ipiranga 2752 - Santa Cecília, CEP 90610-000, Porto Alegre, RS, Brazil.
authors.forename: Samuel
authors.initials: S
authors.lastname: Kaiser
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos, 2600 - anexo - Santana, CEP 90035-003, Porto Alegre, RS, Brazil.
authors.forename: Liliana
authors.initials: L
authors.lastname: Rockenbach
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos, 2600 - anexo - Santana, CEP 90035-003, Porto Alegre, RS, Brazil.
authors.forename: Fabrício
authors.initials: F
authors.lastname: Figueiró
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos, 2600 - anexo - Santana, CEP 90035-003, Porto Alegre, RS, Brazil.
authors.forename: Letícia Scussel
authors.initials: LS
authors.lastname: Bergamin
authors.affiliation: Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos, 2600 - anexo - Santana, CEP 90035-003, Porto Alegre, RS, Brazil.
authors.forename: Fernanda Monte
authors.initials: FM
authors.lastname: da Cunha
authors.affiliation: Laboratório de Farmacologia Aplicada, Faculdade de Farmácia, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Avenida Ipiranga, 6681 - Partenon, CEP 90619-900, Porto Alegre, RS, Brazil.
authors.forename: Fernanda Bueno
authors.initials: FB
authors.lastname: Morrone
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Avenida Ipiranga 2752 - Santa Cecília, CEP 90610-000, Porto Alegre, RS, Brazil.
authors.forename: George González
authors.initials: GG
authors.lastname: Ortega
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos, 2600 - anexo - Santana, CEP 90035-003, Porto Alegre, RS, Brazil; Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos, 2600 - anexo - Santana, CEP 90035-003, Porto Alegre, RS, Brazil. Electronic address: abattastini@gmail.com.
authors.forename: Ana Maria Oliveira
authors.initials: AM
authors.lastname: Battastini
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D020948:Cat's Claw / Q000737:chemistry*; D045744:Cell Line, Tumor; D006027:Glycosides / Q000494:pharmacology*; D006801:Humans; D014315:Triterpenes / Q000737:chemistry / Q000494:pharmacology*; D001749:Urinary Bladder Neoplasms / Q000473:pathology*
chemical_list: D006027:Glycosides; D014315:Triterpenes; C472605:quinovic acid
keywords: Apoptosis; NF-κB; Quinovic acid glycosides purified fraction; Uncaria tomentosa; Urinary bladder cancer