title: Ultrasensitive opto-microfluidic immunosensor integrating gold nanoparticle-enhanced chemiluminescence and highly stable organic photodetector.
abstract: The expensive fabrication of current opto-microfluidic sensors is a barrier to the successful adoption of these devices in point-of-care testing. This work reports a simple inexpensive opto-microfluidic device incorporating a poly(dimethylsiloxane)-glass hybrid microfluidic chip modified with gold nanoparticles and a high-detectivity, high-stability organic photodetector. The enhancing effect of the gold nanoparticles on horseradish peroxidase-luminol-H2O2 chemiluminescence was exploited in rapid single-analyte immunoassays. The limit of detection for 17-β estradiol was 2.5  pg/ml, which is ∼200 times more sensitive than previously reported chemiluminescent immunosensors employing other organic photodetectors. Detection was also demonstrated in complex media, including natural water and blood serum.
authors.forename: Nuno Miguel Matos
authors.initials: NM
authors.lastname: Pires
authors.forename: Tao
authors.initials: T
authors.lastname: Dong
mesh_terms: D006046:Gold / Q000737:chemistry*; D007118:Immunoassay / Q000295:instrumentation; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000295:instrumentation*; D053768:Metal Nanoparticles / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008956:Models, Chemical; D061848:Optical Imaging / Q000295:instrumentation*
chemical_list: D006046:Gold