title: Hybrid recreation by reverse breeding in Arabidopsis thaliana.
abstract: Hybrid crop varieties are traditionally produced by selecting and crossing parental lines to evaluate hybrid performance. Reverse breeding allows doing the opposite: selecting uncharacterized heterozygotes and generating parental lines from them. With these, the selected heterozygotes can be recreated as F1 hybrids, greatly increasing the number of hybrids that can be screened in breeding programs. Key to reverse breeding is the suppression of meiotic crossovers in a hybrid plant to ensure the transmission of nonrecombinant chromosomes to haploid gametes. These gametes are subsequently regenerated as doubled-haploid (DH) offspring. Each DH carries combinations of its parental chromosomes, and complementing pairs can be crossed to reconstitute the initial hybrid. Achiasmatic meiosis and haploid generation result in uncommon phenotypes among offspring owing to chromosome number variation. We describe how these features can be dealt with during a reverse-breeding experiment, which can be completed in six generations (∼1 year).
authors.affiliation: 1] Laboratory of Genetics, Wageningen University, Wageningen, The Netherlands. [2].
authors.forename: Erik
authors.initials: E
authors.lastname: Wijnker
authors.affiliation: Laboratory of Genetics, Wageningen University, Wageningen, The Netherlands.
authors.forename: Laurens
authors.initials: L
authors.lastname: Deurhof
authors.affiliation: Laboratory of Genetics, Wageningen University, Wageningen, The Netherlands.
authors.forename: Jose
authors.initials: J
authors.lastname: van de Belt
authors.affiliation: Rijk Zwaan R&D Fijnaart, Fijnaart, The Netherlands.
authors.forename: C Bastiaan
authors.initials: CB
authors.lastname: de Snoo
authors.affiliation: Laboratory of Genetics, Wageningen University, Wageningen, The Netherlands.
authors.forename: Hetty
authors.initials: H
authors.lastname: Blankestijn
authors.affiliation: Laboratory of Genetics, Wageningen University, Wageningen, The Netherlands.
authors.forename: Frank
authors.initials: F
authors.lastname: Becker
authors.affiliation: 1] Department of Plant Biology, University of California, Davis, Davis, California, USA. [2].
authors.forename: Maruthachalam
authors.initials: M
authors.lastname: Ravi
authors.affiliation: Department of Plant Biology, University of California, Davis, Davis, California, USA.
authors.forename: Simon W L
authors.initials: SW
authors.lastname: Chan
authors.affiliation: Rijk Zwaan R&D Fijnaart, Fijnaart, The Netherlands.
authors.forename: Kees
authors.initials: K
authors.lastname: van Dun
authors.affiliation: Rijk Zwaan R&D Fijnaart, Fijnaart, The Netherlands.
authors.forename: Cilia L C
authors.initials: CL
authors.lastname: Lelivelt
authors.affiliation: Laboratory of Genetics, Wageningen University, Wageningen, The Netherlands.
authors.forename: Hans
authors.initials: H
authors.lastname: de Jong
authors.affiliation: Rijk Zwaan R&D Fijnaart, Fijnaart, The Netherlands.
authors.forename: Rob
authors.initials: R
authors.lastname: Dirks
authors.affiliation: Laboratory of Genetics, Wageningen University, Wageningen, The Netherlands.
authors.forename: Joost J B
authors.initials: JJ
authors.lastname: Keurentjes
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics; D001947:Breeding / Q000379:methods*; D018797:Cell Cycle Proteins / Q000235:genetics; D002678:Chimera; D032461:Chromosomes, Plant; D006238:Haploidy; D006579:Heterozygote; D008540:Meiosis; D030821:Plants, Genetically Modified; D011058:Pollen / Q000235:genetics; D011938:Rec A Recombinases / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D018797:Cell Cycle Proteins; C513034:ATDMC1 protein, Arabidopsis; D011938:Rec A Recombinases