title: [Effect of microRNA-17-92 cluster on the biological characteristics of K562 cells and its mechanisms].
abstract: The objective of this study was to explore the effects of microRNA-17-92 on the biological characteristics of K562 cells. The expression of miR-17-92 in K562 cells transfected with miRNA-17-92 mimic was detected by real time PCR. The effect of microRNA-17-92 on K562 cell proliferation was detected by CCK-8 method. Apoptosis of K562 cells was detected by Annexin V-PI labeling. Cell cycle distribution was determined by using flow cytometry. Western blot was performed to determine the protein levels of Crk. The results indicated that the transfection with miR-17-92 mimic increased expression of mature miR-17-92 in K562 cells. Compared with control group, cell proliferation and cell amount in S-phase of miR-17-92 mimic transfected group significantly increased, cell apoptosis decreased. The expression of signal connector protein Crk was greatly up-regulated in miR-17-92-mimic-transfected K562 cells. It is concluded that miR-17-92 can promote proliferation, inhibit apoptosis and regulate the cell cycle of K562 cells.
authors.affiliation: Department of Hematology, Dalian Medical University, Dalian 116044, Liaoning Province, China; Department of Hematology, General Hospital of Chinese Air Force, Beijing 1000142, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Experimental Hematology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Hui-Yan
authors.initials: HY
authors.lastname: Sun
authors.affiliation: Department of Experimental Hematology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Feng-Jun
authors.initials: FJ
authors.lastname: Xiao
authors.affiliation: Cancer Center, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Qing-Fang
authors.initials: QF
authors.lastname: Li
authors.affiliation: Department of Experimental Hematology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Experimental Hematology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Zi-Kuan
authors.initials: ZK
authors.lastname: Guo
authors.affiliation: Department of Hematology, General Hospital of Chinese Air Force, Beijing 1000142, China. E-mail:wanghengxiang123@yahoo.com.cn.
authors.forename: Heng-Xiang
authors.initials: HX
authors.lastname: Wang
authors.affiliation: Department of Experimental Hematology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Li-Sheng
authors.initials: LS
authors.lastname: Wang
mesh_terms: D017209:Apoptosis; D002453:Cell Cycle; D049109:Cell Proliferation; D015973:Gene Expression Regulation, Leukemic; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D062085:RNA, Long Noncoding; D014162:Transfection
chemical_list: C568997:MIR17HG, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding