title: Identification and cloning of putative water clarification genes of Moringa peregrina (Forssk.) Fiori in E. coli Xl1 blue cells.
abstract: BACKGROUND
  Water purification processes include the use of chemical compounds despite the concern that they may induce diseases. An ecological solution to this dilemma can come from the use of plant seeds for this purpose. Moringa peregrina (Forssk.) Fiori seeds have water clarification ability. Therefore, the aim of this work was to look for certain water clarification genes in M. peregrina.
  
  
  METHODS
  After preparation of M. peregrina callus, mRNA was extracted from these cells. After application of reverse transcriptase, the obtained cDNA (s) were used for PCR amplification of the desired genes using primers based on MO2.1 gene of Moringa oleifera. DNA amplification products were cloned in E. coli Xl1 blue cells and DNA sequences were compared with Mo1,2 gene in M. oleifera.
  
  
  RESULTS
  We obtained 3 PCR products (approximately 200, 300, and 400 bps).
  
  
  CONCLUSIONS
  After comparison of the sequences of 300bp band obtained from M. peregrina with Mo1,2 gene in M. oleifera, it seems that 300bp band is a good candidate to investigate regarding its potential flocculent activity.
authors.affiliation: Department of Biotechnology, Azad University of Sciences and Researches, Tehran, Iran.
authors.forename: Reihaneh
authors.initials: R
authors.lastname: Ghodsi
authors.affiliation: Department of Pharmaceutical Biotechnology, Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Hamid Mirmohammad
authors.initials: HM
authors.lastname: Sadeghi
authors.affiliation: Department of Pharmacognosy, Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Asghari
authors.affiliation: Department of Biotechnology, Azad University of Sciences and Researches, Tehran, Iran.
authors.forename: Sepideh
authors.initials: S
authors.lastname: Torabi
keywords: Flocculent activity; Moringa peregrina; genes cloning