title: In vitro evaluation of cell-seeded chitosan films for peripheral nerve tissue engineering.
abstract: Natural biomaterials have attracted an increasing interest in the field of tissue-engineered nerve grafts, representing a possible alternative to autologous nerve transplantation. With the prospect of developing a novel entubulation strategy for transected nerves with cell-seeded chitosan films, we examined the biocompatibility of such films in vitro. Different types of rat Schwann cells (SCs)--immortalized, neonatal, and adult-of the chitosan substrate. Both cell types were viable on the biomaterial and showed different metabolic activities and proliferation behavior, indicating cell-type-specific cell-biomaterial interaction. Moreover, the cell types also displayed their typical morphology. In cocultures adult SCs used the BMSCs as a feeder layer and no negative interactions between both cell types were detected. Further, the chitosan films allow neurite outgrowth from dissociated sensory neurons, which is additionally supported on film preseeded with SC-BMSC cocultures. The presented chitosan films therefore demonstrate high potential for their use in tissue-engineered nerve grafts.
authors.affiliation: 1 Hannover Medical School, Institute of Neuroanatomy , Hannover, Germany .
authors.forename: Sandra
authors.initials: S
authors.lastname: Wrobel
authors.forename: Sofia Cristina
authors.initials: SC
authors.lastname: Serra
authors.forename: Silvina
authors.initials: S
authors.lastname: Ribeiro-Samy
authors.forename: Nuno
authors.initials: N
authors.lastname: Sousa
authors.forename: Claudia
authors.initials: C
authors.lastname: Heimann
authors.forename: Christina
authors.initials: C
authors.lastname: Barwig
authors.forename: Claudia
authors.initials: C
authors.lastname: Grothe
authors.forename: Antonio Jose
authors.initials: AJ
authors.lastname: Salgado
authors.forename: Kirsten
authors.initials: K
authors.lastname: Haastert-Talini
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D048091:Guided Tissue Regeneration / Q000295:instrumentation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D009416:Nerve Regeneration / Q000502:physiology; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D010525:Peripheral Nerves / Q000166:cytology* / Q000254:growth & development; D051381:Rats; D017208:Rats, Wistar; D012583:Schwann Cells / Q000166:cytology* / Q000502:physiology; D023822:Tissue Engineering / Q000295:instrumentation*; D054457:Tissue Scaffolds
chemical_list: D048271:Chitosan