title: Analysis of flavonoids and hydroxycinnamic acid derivatives in rapeseeds (Brassica napus L. var. napus) by HPLC-PDA--ESI(--)-MS(n)/HRMS.
abstract: A comprehensive description of flavonoids and hydroxycinnamic acid derivatives in Brassica napus L. var. napus seeds is important to improve rapeseed quality. HPLC-PDA-ESI(-)-MS(n)/HRMS has been broadly applied to study phenolic compounds in plants. In the present study, crude phenolic compounds extracted from rapeseed were subjected to column chromatography, alkaline hydrolysis, and HPLC-PDA-ESI(-)-MS(n)/HRMS analysis. A total of 91 flavonoids and hydroxycinnamic acid derivatives were detected, including 39 kaempferol derivatives, 11 isorhamnetin derivatives, 5 quercetin derivatives, 6 flavanols and their oligomers, and 30 hydroxycinnamic acid derivatives. A total of 78 of these compounds were tentatively identified; of these, 55 were reported for the first time in B. napus L. var. napus and 24 were detected for the first time in the genus Brassica. This research enriches our knowledge of the phenolic composition of rapeseed and provides a reliable guide for the selection of rapeseed with valuable breeding potential.
authors.affiliation: Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology and ‡Test Center, Yangzhou University , Yangzhou, 225009 Jiangsu, China.
authors.forename: Yanlin
authors.initials: Y
authors.lastname: Shao
authors.forename: Jinjin
authors.initials: J
authors.lastname: Jiang
authors.forename: Liping
authors.initials: L
authors.lastname: Ran
authors.forename: Chunliang
authors.initials: C
authors.lastname: Lu
authors.forename: Cunxu
authors.initials: C
authors.lastname: Wei
authors.forename: Youping
authors.initials: Y
authors.lastname: Wang
mesh_terms: D029688:Brassica napus / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D003373:Coumaric Acids; D005419:Flavonoids; D010936:Plant Extracts