title: Multiwalled-carbon-nanotubes-based matrix solid-phase dispersion extraction coupled with high-performance liquid chromatography for the determination of honokiol and magnolol in Magnoliae Cortex.
abstract: In this paper, multiwalled-carbon-nanotube-based matrix solid-phase dispersion coupled to HPLC with diode array detection was used to extract and determine honokiol and magnolol from Magnoliae Cortex. The extraction efficiency of the multiwalled-carbon-nanotube-based matrix solid-phase dispersion was studied and optimized as a function of the amount of dispersing sorbent, volume of elution solvent, and flow rate of elution solvent, with the aid of response surface methodology. An amount of 0.06 g of carboxyl-modified multiwalled carbon nanotubes and 1.5 mL of methanol at a flow rate of 1.1 mL/min were selected. The method obtained good linearity (r(2) > 0.9992) and precision (RSD < 4.7%) for honokiol and magnolol, with limits of detection of 0.045 and 0.087 μg/mL, respectively. The recoveries obtained from analyzing in triplicate spiked samples were determined to be from 90.23 to 101.10% and the RSDs from 3.5 to 4.8%. The proposed method that required less samples and reagents was simpler and faster than Soxhlet and maceration extraction methods. The optimized method was applied for analyzing five real samples collected from different cultivated areas.
authors.affiliation: Institute of Medicine and Drug Research, Qiqihar Medical University, Qiqihar, P.R. China; Basic Medical Science College, Qiqihar Medical University, Qiqihar, P.R. China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Hong
authors.forename: Jianhua
authors.initials: J
authors.lastname: Liu
authors.forename: Guangze
authors.initials: G
authors.lastname: Mu
authors.forename: Huan
authors.initials: H
authors.lastname: Cong
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.forename: Defu
authors.initials: D
authors.lastname: Cai
mesh_terms: D001713:Biphenyl Compounds / Q000032:analysis* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017705:Lignans / Q000032:analysis* / Q000302:isolation & purification*; D031566:Magnolia / Q000737:chemistry*; D037742:Nanotubes, Carbon; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D000498:Allyl Compounds; D010636:Phenols
chemical_list: D001713:Biphenyl Compounds; D017705:Lignans; D037742:Nanotubes, Carbon; D010936:Plant Extracts; C005498:magnolol; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols
keywords: Box-Behnken design; Honokiol; Magnolol; Matrix solid-phase dispersion; Response surface methodology