title: Apoptosis induction in duck tissues during duck hepatitis A virus type 1 infection.
abstract: To investigate the role of apoptosis in duck viral hepatitis pathogenesis, 4- and 21-d-old ducks were inoculated with duck hepatitis A virus serotype 1 and killed at 2, 6, 12, 24, and 48 h postinfection. TdT-mediated dUTP nick-end labeling was used to detect apoptosis cells. Expression profiles of apoptosis-related genes including caspase-3, -8, -9, and Bcl-2 in spleen, bursa of Fabricius, liver, and the quantity of virus in blood were examined using real-time PCR. The TdT-mediated dUTP nick-end labeling analysis indicated there was a significant difference of apoptotic cells between treatments and controls. The same difference also appeared in virus amount variation in blood during infection. Gene expression analysis revealed that the apoptosis-related gene expression profile was different in the 2 groups, and also different between various organs. This study suggested that apoptosis may play an important role in duck hepatitis A virus serotype 1 infection, and apoptosis suppression might facilitate virus multiplication, resulting in the highest virus concentration in the host.
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: X D
authors.initials: XD
authors.lastname: Sheng
authors.forename: W P
authors.initials: WP
authors.lastname: Zhang
authors.forename: Q R
authors.initials: QR
authors.lastname: Zhang
authors.forename: C Q
authors.initials: CQ
authors.lastname: Gu
authors.forename: X Y
authors.initials: XY
authors.lastname: Hu
authors.forename: G F
authors.initials: GF
authors.lastname: Cheng
mesh_terms: D000818:Animals; D017209:Apoptosis; D002060:Bursa of Fabricius / Q000502:physiology / Q000821:virology; D004372:Ducks; D005786:Gene Expression Regulation; D004371:Hepatitis Virus, Duck / Q000302:isolation & purification / Q000502:physiology*; D020287:In Situ Nick-End Labeling / Q000662:veterinary; D008099:Liver / Q000502:physiology / Q000821:virology; D009928:Organ Specificity; D010850:Picornaviridae Infections / Q000235:genetics / Q000503:physiopathology / Q000662:veterinary* / Q000821:virology; D011201:Poultry Diseases / Q000235:genetics / Q000503:physiopathology* / Q000821:virology; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D013154:Spleen / Q000502:physiology / Q000821:virology; D014779:Virus Replication
keywords: apoptosis; duck; duck hepatitis A virus type 1; gene expression