title: Argininosuccinate synthetase as a plasma biomarker of liver injury after acetaminophen overdose in rodents and humans.
abstract: BACKGROUND
  New biomarkers are needed in acetaminophen (APAP) hepatotoxicity. Plasma argininosuccinate synthetase (ASS) is a promising candidate.
  
  
  OBJECTIVE
  Characterize ASS in APAP hepatotoxicity.
  
  
  METHODS
  ASS was measured in plasma from rodents and humans with APAP hepatotoxicity.
  
  
  RESULTS
  In mice, ASS increased before injury, peaked before alanine aminotransferase (ALT) and decreased rapidly. Fischer rats had a greater increase in ASS relative to ALT. Patients with abnormal liver test results had very high ASS compared to controls. ASS appeared to increase early in some patients, and declined rapidly in all.
  
  
  CONCLUSIONS
  ASS may be a useful biomarker of acute cell death in APAP hepatotoxicity.
authors.affiliation: Department of Pharmacology, Toxicology, and Therapeutics, University of Kansas Medical Center , Kansas City, KS , USA .
authors.forename: Mitchell R
authors.initials: MR
authors.lastname: McGill
authors.forename: Mengde
authors.initials: M
authors.lastname: Cao
authors.forename: Archie
authors.initials: A
authors.lastname: Svetlov
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Sharpe
authors.forename: C David
authors.initials: CD
authors.lastname: Williams
authors.forename: Steven C
authors.initials: SC
authors.lastname: Curry
authors.forename: Anwar
authors.initials: A
authors.lastname: Farhood
authors.forename: Hartmut
authors.initials: H
authors.lastname: Jaeschke
authors.forename: Stanislav I
authors.initials: SI
authors.lastname: Svetlov
mesh_terms: D000082:Acetaminophen / Q000506:poisoning*; D000818:Animals; D001124:Argininosuccinate Synthase / Q000097:blood*; D062787:Drug Overdose / Q000097:blood*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D000082:Acetaminophen; D001124:Argininosuccinate Synthase