title: PV.1 suppresses the expression of FoxD5b during neural induction in Xenopus embryos.
abstract: Suppression of bone morphogenetic protein (BMP) signaling induces neural induction in the ectoderm of developing embryos. BMP signaling inhibits eural induction via the expression of various neural suppressors. Previous research has demonstrated that the ectopic expression of dominant negative BMP receptors (DNBR) reduces the expression of target genes down-stream of BMP and leads to neural induction. Additionally, gain-of-function experiments have shown that BMP downstream target genes such as MSX1, GATA1b and Vent are involved in the suppression of neural induction. For example, the Vent1/2 genes are involved in the suppression of Geminin and Sox3 expression in the neural ectodermal region of embryos. In this paper, we investigated whether PV.1, a BMP downstream target gene, negatively regulates the expression of FoxD5b, which plays a role in maintaining a neural progenitor population. A promoter assay and a cyclohexamide experiment demonstrated that PV.1 negatively regulates FoxD5b expression.
authors.affiliation: Department of Biochemistry, Institute of Cell Differentiation and Aging, College of Medicine, Hallym University, Chuncheon 200-702, Korea.
authors.forename: Jaeho
authors.initials: J
authors.lastname: Yoon
authors.forename: Jung-Ho
authors.initials: JH
authors.lastname: Kim
authors.forename: Sung Chan
authors.initials: SC
authors.lastname: Kim
authors.forename: Jae-Bong
authors.initials: JB
authors.lastname: Park
authors.forename: Jae-Yong
authors.initials: JY
authors.lastname: Lee
authors.forename: Jaebong
authors.initials: J
authors.lastname: Kim
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D051858:Forkhead Transcription Factors / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D020868:Gene Silencing; D018398:Homeodomain Proteins / Q000502:physiology*; D055495:Neurogenesis; D020218:Response Elements; D029867:Xenopus Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics / Q000378:metabolism
chemical_list: D051858:Forkhead Transcription Factors; C495065:FoxD5b protein, Xenopus; D018398:Homeodomain Proteins; D029867:Xenopus Proteins; C112507:ventx1.2 protein, Xenopus