title: Multiplex SYBR® green-real time PCR (qPCR) assay for the detection and differentiation of Bartonella henselae and Bartonella clarridgeiae in cats.
abstract: A novel SYBR® green-real time polymerase chain reaction (qPCR) was developed to detect two Bartonella species, B. henselae and B. clarridgeiae, directly from blood samples. The test was used in blood samples obtained from cats living in animal shelters in Southern Brazil. Results were compared with those obtained by conventional PCR targeting Bartonella spp. Among the 47 samples analyzed, eight were positive using the conventional PCR and 12 were positive using qPCR. Importantly, the new qPCR detected the presence of both B. henselae and B. clarridgeiae in two samples. The results show that the qPCR described here may be a reliable tool for the screening and differentiation of two important Bartonella species.
authors.affiliation: Laboratório de Microbiologia Molecular, Universidade Feevale, 93352-000Novo HamburgoRS, Brazil, Laboratório de Microbiologia Molecular, Universidade Feevale, RS 239 n° 2755, 93352-000 Novo Hamburgo, RS, Brazil.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Staggemeier
authors.affiliation: Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Brazil, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Brazil.
authors.forename: Diogo André
authors.initials: DA
authors.lastname: Pilger
authors.affiliation: Laboratório de Microbiologia Molecular, Universidade Feevale, 93352-000Novo HamburgoRS, Brazil, Laboratório de Microbiologia Molecular, Universidade Feevale, RS 239 n° 2755, 93352-000 Novo Hamburgo, RS, Brazil.
authors.forename: Fernando Rosado
authors.initials: FR
authors.lastname: Spilki
authors.affiliation: Laboratório de Microbiologia Molecular, Universidade Feevale, 93352-000Novo HamburgoRS, Brazil, Laboratório de Microbiologia Molecular, Universidade Feevale, RS 239 n° 2755, 93352-000 Novo Hamburgo, RS, Brazil.
authors.forename: Vlademir Vicente
authors.initials: VV
authors.lastname: Cantarelli
mesh_terms: D000818:Animals; D001473:Bartonella / Q000235:genetics* / Q000302:isolation & purification*; D001474:Bartonella Infections / Q000382:microbiology / Q000662:veterinary*; D018416:Bartonella henselae / Q000235:genetics / Q000302:isolation & purification; D002371:Cat Diseases / Q000382:microbiology*; D002415:Cats; D004269:DNA, Bacterial / Q000097:blood*; D060885:Multiplex Polymerase Chain Reaction / Q000662:veterinary*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary*; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial