title: Highly sensitive determination of copper in HeLa cell using capillary electrophoresis combined with a simple cell extraction treatment.
abstract: A new separation system of capillary electrophoresis (CE1) for the highly sensitive determination of copper was established by using ethylenediaminetetraacetic acid (EDTA) as a complexing agent and employing cetyltrimethylammonium chloride (CTAC) as a capillary inner wall modifier. Benefitted from the combination of field-enhanced sample injection (FESI) method, a limit of detection (LOD) of 2.7 nM was obtained, which was much lower than that of the conventional methods. This made it possible to determine trace copper in HeLa cell only by a simple cell extraction (CE2) treatment. Two copper-extraction methods-acid-hydrolysis and freeze-thaw-were compared. Limited by the requirement of low ion strength in FESI, only the extract using freeze-thaw could be successfully applied in the determination. The effectiveness assessment of this CE(2)-FESI method was adopted by inductively coupled plasma-atomic emission spectrometry (ICP-AES) as a gold standard.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, PR China.
authors.forename: Lingchen
authors.initials: L
authors.lastname: Meng
authors.affiliation: Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, PR China.
authors.forename: Ziyuan
authors.initials: Z
authors.lastname: Fang
authors.affiliation: Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Lin
authors.affiliation: Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, PR China.
authors.forename: Meixian
authors.initials: M
authors.lastname: Li
authors.affiliation: Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, PR China. Electronic address: zwzhu@pku.edu.cn.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Zhu
mesh_terms: D002138:Calibration; D003300:Copper / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection
chemical_list: D003300:Copper
keywords: Capillary electrophoresis; Copper; EDTA; HeLa cells