title: Low-protein diet in adult male rats has long-term effects on metabolism.
abstract: Nutritional insults during developmental plasticity have been linked with metabolic diseases such as diabetes in adulthood. We aimed to investigate whether a low-protein (LP) diet at the beginning of adulthood is able to program metabolic disruptions in rats. While control rats ate a normal-protein (23%; NP group) diet, treated rats were fed a LP (4%; LP group) diet from 60 to 90 days of age, after which an NP diet was supplied until they were 150 days old. Plasma levels of glucose and insulin, autonomous nervous system (ANS), and pancreatic islet function were then evaluated. Compared with the NP group, LP rats exhibited unchanged body weight and reduced food intake throughout the period of protein restriction; however, after the switch to the NP diet, hyperphagia of 10% (P<0.05), and catch-up growth of 113% (P<0.0001) were found. The LP rats showed hyperglycemia, insulin resistance, and higher fat accretion than the NP rats. While the sympathetic tonus from LP rats reduced by 28%, the vagus tonus increased by 21% (P<0.05). Compared with the islets from NP rats, the glucose insulinotropic effect as well as cholinergic and adrenergic actions was unaltered in the islets from LP rats. Protein restriction at the beginning of adulthood induced unbalanced ANS activity and fat tissue accretion later in life, even without functional disturbances in the pancreatic islets.
authors.affiliation: Laboratory of Secretion Cell Biology, Department of Cell Biology and Genetics, Block H67, Room 19, State University of Maringá, Colombo Avenue 5970, 87020-900 Maringá, Parana, Brazil UPSP-EGEAL Institut Polytechnique LaSalle de Beauvais, Beauvais Cedex, France.
authors.forename: Ananda
authors.initials: A
authors.lastname: Malta
authors.forename: Júlio Cezar
authors.initials: JC
authors.lastname: de Oliveira
authors.forename: Tatiane Aparecida da Silva
authors.initials: TA
authors.lastname: Ribeiro
authors.forename: Laize Peron
authors.initials: LP
authors.lastname: Tófolo
authors.forename: Luiz Felipe
authors.initials: LF
authors.lastname: Barella
authors.forename: Kelly Valério
authors.initials: KV
authors.lastname: Prates
authors.forename: Rosiane Aparecida
authors.initials: RA
authors.lastname: Miranda
authors.forename: Ghada
authors.initials: G
authors.lastname: Elmhiri
authors.forename: Claudinéia Conationi da Silva
authors.initials: CC
authors.lastname: Franco
authors.forename: Aryane Rodrigues
authors.initials: AR
authors.lastname: Agostinho
authors.forename: Amanda Bianchi
authors.initials: AB
authors.lastname: Trombini
authors.forename: Audrei
authors.initials: A
authors.lastname: Pavanello
authors.forename: Clarice
authors.initials: C
authors.lastname: Gravena
authors.forename: Latifa
authors.initials: L
authors.lastname: Abdennebi-Najar
authors.forename: Paulo Cezar de Freitas
authors.initials: PC
authors.lastname: Mathias
mesh_terms: D000818:Animals; D001341:Autonomic Nervous System / Q000503:physiopathology*; D001724:Birth Weight / Q000187:drug effects / Q000502:physiology; D001786:Blood Glucose / Q000378:metabolism; D002478:Cells, Cultured; D018753:Diet, Protein-Restricted / Q000009:adverse effects*; D004044:Dietary Proteins / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000187:drug effects / Q000294:innervation* / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D004044:Dietary Proteins; D007328:Insulin
keywords: adulthood; autonomous nervous system; pancreatic islets; protein restriction