title: Digital colour tone for fluorescence sensing: a direct comparison of intensity, ratiometric and hue based quantification.
abstract: The hue parameter of HSV colour-space for digital imaging is shown to be accessible for convenient quantitative fluorescence imaging. A commercially available pH probe was utilized in solution and incorporated into optical μ-sensors for microscopy applications.
authors.affiliation: Department of Applied Physics, Chalmers University of Technology, Gothenburg, Sweden. hakonen@chalmers.se.
authors.forename: Aron
authors.initials: A
authors.lastname: Hakonen
authors.forename: Jonathon E
authors.initials: JE
authors.lastname: Beves
authors.forename: Niklas
authors.initials: N
authors.lastname: Strömberg