title: Hemodialysis-induced changes in the blood composition affect function of the endothelium.
abstract: Hemodialysis induces oxidative stress causing intravascular inflammation, which may cause endothelial dysfunction. We evaluated how hemodialysis-induced changes in blood affect the function of endothelial cells in in vitro culture. Serum samples were collected from 42 uremic patients treated with hemodialysis, one before the start of dialysis and the other one at the end of session. All patients were dialysed with polysulfone dialyzer. Concentrations of the inflammatory molecules carbonyl protein and metabolites of NO synthesis were measured in blood. Additionally, the effect of the serum obtained before and after dialysis on the function of endothelial cells in in vitro culture was studied. Hemodialysis caused increase of monocyte chemoattractant protein (MCP)-1 (+17%), hepatocyte growth factor (+91%), and pentraxin-3 (+30%) concentration in serum. Concentration of carbonyl protein was decreased by 30%. Decrease of blood level of asymmetric dimethylarginine (-25%) and nitrate/nitrites (-62%) was observed. Serum obtained after hemodialysis stimulated proliferation of endothelial cells (+10%) and synthesis of MCP-1(+11%) in these cells. Hemodialysis-induced intravascular inflammation changes the function of endothelial cells, which may lead to acceleration of atherosclerosis.
authors.affiliation: Department of Pathophysiology, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Weronika
authors.initials: W
authors.lastname: Oleśkowska-Florek
authors.forename: Alicja
authors.initials: A
authors.lastname: Połubinska
authors.forename: Ewa
authors.initials: E
authors.lastname: Baum
authors.forename: Monika
authors.initials: M
authors.lastname: Matecka
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Pyda
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Pawlaczyk
authors.forename: Andrzej
authors.initials: A
authors.lastname: Bręborowicz
mesh_terms: D004730:Endothelium, Vascular / Q000503:physiopathology*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology; D006435:Renal Dialysis / Q000009:adverse effects*; D014511:Uremia / Q000097:blood* / Q000628:therapy*; D014652:Vascular Diseases / Q000097:blood*
keywords: Hemodialysis; endothelium; inflammation