title: Pharmacological analysis of response latency in the hot plate test following whole-body static magnetic field-exposure in the snail Helix pomatia.
abstract: OBJECTIVE
  To study the effect of single, 30-min long, whole-body, homogeneous static magnetic field (SMF)-exposure of magnetic induction 147 ± 3 mT on the response latency of the snail Helix pomatia.
  
  
  METHODS
  The response was investigated using the hot plate test.
  
  
  RESULTS
  The effect caused by exposure to SMF was compared to sham-exposure and resulted in significant differences (up to 47.1%, p < 0.001). The response latency depended on the day-night cycle; response latency was higher by 51.2% (p < 0.001) during the night. This trend also held for SMF-exposure (28.6%, p < 0.001). Serotonin alone increased response latency (55.7%, p < 0.001), whereas serotonin antagonist tryptamine decreased it (- 97.8%, p < 0.001). Using naloxone, response latency decreased (- 52.5%, p < 0.001); however both SMF-exposure and serotonin in combination with naloxone rose it back to above the control level (116.9%, p < 0.001 or 150.2%, p < 0.001, respectively).
  
  
  CONCLUSIONS
  This study provides evidence that SMF-exposure mediates peripheral thermal nociceptive threshold by affecting the serotonerg as well as the opioiderg system.
authors.affiliation: Department of Experimental Zoology, Balaton Limnological Institute, MTA Centre for Ecological Research , Tihany , Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Hernádi
authors.forename: János F
authors.initials: JF
authors.lastname: László
mesh_terms: D000701:Analgesics, Opioid / Q000494:pharmacology; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D002940:Circadian Rhythm / Q000187:drug effects; D004347:Drug Interactions; D006372:Helix, Snails / Q000187:drug effects* / Q000502:physiology*; D006358:Hot Temperature; D060526:Magnetic Fields; D011930:Reaction Time / Q000187:drug effects* / Q000502:physiology; D012701:Serotonin / Q000494:pharmacology
chemical_list: D000701:Analgesics, Opioid; D012701:Serotonin
keywords: Helix pomatia; analgesia; naloxone; nociception; serotonin; static magnetic field (SMF); tryptamine