title: Hannaella pagnoccae sp. nov., a tremellaceous yeast species isolated from plants and soil.
abstract: Several independent surveys of yeasts associated with different plant materials and soil led to the proposal of a novel yeast species belonging to the Tremellales clade (Agaricomycotina, Basidiomycota). Analysis of the sequences of the D1/D2 domains and internal transcribed spacer region of the large subunit of the rRNA gene suggested affinity to a phylogenetic lineage that includes Hannaella coprosmaensis, Hannaella oryzae and Hannaella sinensis. Thirty-two isolates were obtained from different sources, including bromeliads, nectar of Heliconia psittacorum (Heliconiaceae), flowers of Pimenta dioica (Myrtaceae), roots and leaves of sugar cane (Saccharum spp.) in Brazil, leaves of Cratoxylum maingayi, Arundinaria pusilla and Vitis vinifera in Thailand, soil samples in Taiwan, and prairie soil in the USA. Sequence analysis of the D1/D2 domains of the large subunit of the rRNA gene showed that the novel species differs from Hannaella coprosmaensis and Hannaella oryzae by 36 and 46 nt substitutions, respectively. A novel species is suggested to accommodate these isolates, for which the name Hannaella pagnoccae sp. nov. is proposed. The type strain is BI118(T) ( = CBS 11142(T) = ATCC MYA-4530(T)).
authors.affiliation: Programa de Pós Graduação em Diversidade Biológica e Conservação nos Trópicos, Universidade Federal de Alagoas, Maceió - AL, Brazil.|Setor de Genética/ICBS, Universidade Federal de Alagoas, Maceió - AL, Brazil.
authors.forename: Melissa Fontes
authors.initials: MF
authors.lastname: Landell
authors.affiliation: Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte - MG, 31270-901, Brazil.
authors.forename: Luciana R
authors.initials: LR
authors.lastname: Brandão
authors.affiliation: Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte - MG, 31270-901, Brazil.
authors.forename: Anne C
authors.initials: AC
authors.lastname: Barbosa
authors.affiliation: National Reference Laboratory for Tuberculosis, Centro de Referência Professor Hélio Fraga, Escola Nacional de Saúde Pública, Fiocruz - RJ, Brazil.
authors.forename: Jesus P
authors.initials: JP
authors.lastname: Ramos
authors.affiliation: Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte - MG, 31270-901, Brazil.
authors.forename: Silvana V B
authors.initials: SVB
authors.lastname: Safar
authors.affiliation: Departamento de Química, Centro Federal de Educação Tecnológica de Minas Gerais, Belo Horizonte - MG, 30421-169, Brazil.
authors.forename: Fatima C O
authors.initials: FCO
authors.lastname: Gomes
authors.affiliation: Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte - MG, 31270-901, Brazil.
authors.forename: Francisca M P
authors.initials: FMP
authors.lastname: Sousa
authors.affiliation: Laboratorio de Microbiologia Ambiental e Biotecnologia, Universidade Federal do Tocantins, Palmas, TO 77020-220, Brazil.
authors.forename: Paula B
authors.initials: PB
authors.lastname: Morais
authors.affiliation: Setor de Genética/ICBS, Universidade Federal de Alagoas, Maceió - AL, Brazil.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Broetto
authors.affiliation: Instituto Nacional do Câncer, Rio de Janeiro - RJ, Brazil.
authors.forename: Orílio
authors.initials: O
authors.lastname: Leoncini
authors.affiliation: Instituto de Microbiologia Prof. Paulo de Goes, Universidade Federal do Rio de Janeiro, Rio de Janeiro - RJ, Brazil.
authors.forename: José Roberto
authors.initials: JR
authors.lastname: Ribeiro
authors.affiliation: Bioscience Department, Thailand Institute of Scientific and Technological Research (TISTR), Klong Luang, Pathunthani 12120, Thailand.
authors.forename: Bundit
authors.initials: B
authors.lastname: Fungsin
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Masako
authors.initials: M
authors.lastname: Takashima
authors.affiliation: Department of Biotechnology, NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE), Chiba, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakase
authors.affiliation: Department of Applied Science, National Hsinchu University of Education, Hsinchu, 300 Taiwan.
authors.forename: Ching-Fu
authors.initials: CF
authors.lastname: Lee
authors.affiliation: Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre - RS, Brazil.
authors.forename: Marilene H
authors.initials: MH
authors.lastname: Vainstein
authors.affiliation: Rosenstiel School of Marine and Atmospheric Science, University of Miami, Key Biscayne, FL 33149, USA.
authors.forename: Jack W
authors.initials: JW
authors.lastname: Fell
authors.affiliation: Rosenstiel School of Marine and Atmospheric Science, University of Miami, Key Biscayne, FL 33149, USA.
authors.forename: Gloria
authors.initials: G
authors.lastname: Scorzetti
authors.affiliation: Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, OK 74078, USA.
authors.forename: Helen S
authors.initials: HS
authors.lastname: Vishniac
authors.affiliation: Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte - MG, 31270-901, Brazil.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Rosa
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal do Rio Grande do Sul, Porto Alegre - RS, Brazil.|Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre - RS, Brazil.
authors.forename: Patricia
authors.initials: P
authors.lastname: Valente
mesh_terms: D001483:Base Sequence; D001487:Basidiomycota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D035264:Flowers / Q000382:microbiology; D032427:Heliconiaceae / Q000382:microbiology*; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D027822:Myrtaceae / Q000382:microbiology*; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology; D018517:Plant Roots / Q000382:microbiology; D031786:Saccharum / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013624:Taiwan
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer