title: Controlled assembly of peptide-functionalized gold nanoparticles for label-free detection of blood coagulation Factor XIII activity.
abstract: A highly sensitive label-free assay for the determination of blood coagulation Factor XIII activity is demonstrated through the controlled assembly of peptide-functionalized gold nanoparticles (AuNPs). Activated Factor XIII catalyzes the formation of covalent crosslinking between peptide chains through ε-(γ-glutamyl)-lysine bonds leading to the aggregation of the AuNPs and consequently a red-shift of the localized surface plasmon resonance. The selective engineering of nanoscale order over AuNP crosslinking via the formation of isopeptide bonds provides a new approach toward the design of nanoassemblies with precise control on the molecular level. The colorimetric assay reported here provides direct qualitative and quantitative analysis of Factor XIII activity with a limit of detection of 0.01 U mL(-1).
authors.affiliation: Department of Materials, Department of Bioengineering and Institute for Biomedical Engineering, Imperial College London, London SW7 2AZ, UK. m.stevens@imperial.ac.uk r.chandrawati@imperial.ac.uk.
authors.forename: Rona
authors.initials: R
authors.lastname: Chandrawati
authors.forename: Molly M
authors.initials: MM
authors.lastname: Stevens
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D005176:Factor XIII / Q000378:metabolism*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*
chemical_list: D010455:Peptides; D006046:Gold; D005176:Factor XIII