title: [Effect of uighur medicine abnormal savda munzip on human hypertrophic scar fibroblasts in vitro].
abstract: OBJECTIVE
  To evaluate in vitro effect of abnormal savda munziq (ASMq) on the proliferation and apoptosis of human hypertrophic scar fibroblasts (HSFs).
  
  
  METHODS
  HSFs were divided into six groups to receive different treatments as group A (blank control group), group B-E (ASMq in different concentration), and group F(5-Fu). Each group contains six specimens. The HSFs were cultured in vitro. After culture for 48 hours, the CCK8 test and flow cytometry methods were used to detect the proliferation, cell cycle and apoptosis.
  
  
  RESULTS
  The proliferation of HSFs in the B, C, D and E groups was inhibited at G2/M period, while it was inhibited at G0/S period in group F (P < 0.05). The inhibition effect of ASMq (0.1-1.0 mg/ml) on the fibroblasts enhanced in a concentration-dependent manner. Flow cytometry analysis with annexin V-FITC and PI staining confirmed the apoptotic. When HSFs were exposed to ASMq at 1.0 mg/ml (group E) for 48 h, the percentage of apoptotic cells increased to (43.7 +/- 2.58)%, which was significantly higher than that of blank control group (2.2 +/- 0.59)%. The induced apoptosis effect was also increased in a concentration-dependent manner.
  
  
  CONCLUSIONS
  ASMq has a inhibitory effect on the proliferation and an enhancement effect on the apoptosis of fibroblast. ASMq could be used as an effective drug for treatment of hypertrophic scar.
authors.forename: Wei-Cheng
authors.initials: WC
authors.lastname: Gao
authors.forename: Hu-Jun
authors.initials: HJ
authors.lastname: Wang
authors.forename: Xing
authors.initials: X
authors.lastname: Qiao
authors.forename: Juan
authors.initials: J
authors.lastname: Ma
authors.forename: Jin
authors.initials: J
authors.lastname: Du
authors.forename: Shao-Lin
authors.initials: SL
authors.lastname: Ma
mesh_terms: D017209:Apoptosis; D002453:Cell Cycle / Q000187:drug effects* / Q000502:physiology; D002455:Cell Division; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D017439:Cicatrix, Hypertrophic / Q000473:pathology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D008518:Medicine, East Asian Traditional