title: [Detection and analysis of HBV in polymorphonuclear neutrophils of patients with hepatitis B].
abstract: OBJECTIVE
  To study the infection and replication of hepatitis B virus (HBV) in polymorphonuclear neutrophils of the patients with chronic hepatitis B.
  
  
  METHODS
  The neutrophils were isolated and purified by neutrophil isolation medium, and the loads of HBV-DNA in neutrophils were detected by PCR.
  
  
  RESULTS
  Positive detection rate of HBV-DNA in neutrophils of chronic hepatitis B patients was 30.95% (13/42), and among them, the HBeAg positive and HBeAg negative ones were 52.94% (9/17) and 16.00%(4/25), respectively. After treatment of adefovir, the positive rate of HBV-DNA in neutrophils of chronic hepatitis B patients was 9.52% (4/42), and among them, the HBeAg positive and HBeAg negative ones were 17.65%(3/17) and 4.00%(1/25), respectively. There were significantly different positive rates in the patients before and after treatment (P<0.05).
  
  
  CONCLUSIONS
  HBV can infect the neutrophils of the patients with chronic hepatitis B to achieve extrahepatic occult infection. Adefovir has a good inhibitory effect against the latent or hidden HBV in neutrophils.
authors.affiliation: Department of Aetiology and Immunology, Medical College, Anhui University of Science and Technology, Huainan 232001, China.
authors.forename: Zhongyan
authors.initials: Z
authors.lastname: Han
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000627:therapeutic use; D000328:Adult; D004279:DNA, Viral / Q000097:blood; D005260:Female; D006513:Hepatitis B e Antigens / Q000032:analysis; D006515:Hepatitis B virus / Q000302:isolation & purification*; D019694:Hepatitis B, Chronic / Q000188:drug therapy / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000821:virology*; D063065:Organophosphonates / Q000627:therapeutic use
chemical_list: D004279:DNA, Viral; D006513:Hepatitis B e Antigens; D063065:Organophosphonates; C053001:adefovir; D000225:Adenine