title: Influence of co-existed benzo[a]pyrene and copper on the cellular characteristics of Stenotrophomonas maltophilia during biodegradation and transformation.
abstract: Microbial remediation has been proposed as a promising technique to remove pollutions, however, its application has been hindered by the lack of understanding the mechanisms involved in contaminants conversion and the influence of pollutants on cellular characteristics. To address this problem, biodegradation and transformation of BaP-Cu(II) by Stenotrophomonas maltophilia, along with interactions of these pollutants with microbial cells through FCM assay were investigated. The results indicated that BaP and Cu(II) were rapidly removed by S. maltophilia on the 1st d, but only less than 10% BaP was broken down due to temporary store in cells, instead of being decomposed immediately. The key ATP enzymes in cells were then activated by BaP to promote bacteria to further decompose BaP. Stimulation of co-existed contaminants strengthened cell membrane permeability and altered cell structure, but a higher esterase activity and DNA in cells of S. maltophilia were still retained.
authors.affiliation: Department of Ecology, Jinan University, Guangzhou 510632, China.
authors.forename: Shuona
authors.initials: S
authors.lastname: Chen
authors.affiliation: Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, College of Environment and Energy, South China University of Technology, Guangzhou 510006, China. Electronic address: huayin@scut.edu.cn.
authors.forename: Hua
authors.initials: H
authors.lastname: Yin
authors.affiliation: Department of Environmental Engineering, Jinan University, Guangzhou 510632, China.
authors.forename: Jinshao
authors.initials: J
authors.lastname: Ye
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Peng
authors.affiliation: Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, College of Environment and Energy, South China University of Technology, Guangzhou 510006, China.
authors.forename: Zehua
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, College of Environment and Energy, South China University of Technology, Guangzhou 510006, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Dang
authors.affiliation: Department of Environmental Engineering, Jinan University, Guangzhou 510632, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Chang
mesh_terms: D001564:Benzo(a)pyrene / Q000378:metabolism* / Q000633:toxicity; D001673:Biodegradation, Environmental; D002462:Cell Membrane / Q000187:drug effects; D003300:Copper / Q000378:metabolism* / Q000633:toxicity; D004785:Environmental Pollutants / Q000633:toxicity*; D005434:Flow Cytometry; D020615:Stenotrophomonas maltophilia / Q000187:drug effects / Q000378:metabolism*
chemical_list: D004785:Environmental Pollutants; D001564:Benzo(a)pyrene; D003300:Copper
keywords: Benzo[a]pyrene; Cellular characteristics; Copper; Flow cytometry; Stenotrophomonas maltophilia