title: 2D spectroscopy study of water-soluble chlorophyll-binding protein from Lepidium virginicum.
abstract: Water-soluble chlorophyll-binding proteins (WSCPs) are interesting model systems for the study of pigment-pigment and pigment-protein interactions. While class IIa WSCP has been extensively studied by spectroscopic and theoretical methods, a comprehensive spectroscopic study of class IIb WSCP was lacking so far despite the fact that its structure was determined by X-ray crystallography. In this paper, results of two-dimensional electronic spectroscopy applied to the class IIb WSCP from Lepidium virginicum are presented. Global analysis of 2D data allowed determination of energy levels and excitation energy transfer pathways in the system. Some additional pathways, not present in class IIa WSCP, were observed. The data were interpreted in terms of a model comprising two interacting chlorophyll dimers. In addition, oscillatory signals were observed and identified as coherent beatings of vibrational origin.
authors.affiliation: Department of Chemical Physics, Lund University , P.O. Box 124, SE-221-00 Lund, Sweden.
authors.forename: Jan
authors.initials: J
authors.lastname: Alster
authors.forename: Heiko
authors.initials: H
authors.lastname: Lokstein
authors.forename: Jakub
authors.initials: J
authors.lastname: Dostál
authors.forename: Akira
authors.initials: A
authors.lastname: Uchida
authors.forename: Donatas
authors.initials: D
authors.lastname: Zigmantas
mesh_terms: D002734:Chlorophyll / Q000737:chemistry* / Q000378:metabolism; D029686:Lepidium / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D011485:Protein Binding; D012995:Solubility; D013053:Spectrophotometry; D014867:Water / Q000737:chemistry
chemical_list: D010940:Plant Proteins; D014867:Water; D002734:Chlorophyll