title: High grade glioblastoma is associated with aberrant expression of ZFP57, a protein involved in gene imprinting, and of CPT1A and CPT1C that regulate fatty acid metabolism.
abstract: The diagnosis of glioblastoma is still based on tumor histology, but emerging molecular diagnosis is becoming an important part of glioblastoma classification. Besides the well-known cell cycle-related circuitries that are associated with glioblastoma onset and development, new insights may be derived by looking at pathways involved in regulation of epigenetic phenomena and cellular metabolism, which may both be highly deregulated in cancer cells. We evaluated if in glioblastoma patients the high grade of malignancy could be associated with aberrant expression of some genes involved in regulation of epigenetic phenomena and lipid metabolism. We measured the mRNA levels of ZFP57, TRIM28, CPT1A, CPT1B, and CPT1C in a cohort of 80 patients divided in two groups: grade II and grade IV. We evidenced that high grade glioblastoma is associated with increased level of ZFP57, a protein involved in gene imprinting, and aberrant expression of CPT1A and CPT1C, regulators of fatty acid oxidation. Our study may pave the way to identify new markers that could be potentially useful for diagnosis and/or prognosis of glioblastoma.
authors.affiliation: Institute of Biomedicine and Bioresources; CNR; Naples, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Cirillo
authors.affiliation: Institute of Biomedicine and Bioresources; CNR; Naples, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Di Salle
authors.affiliation: Institute of Biomedicine and Bioresources; CNR; Naples, Italy.
authors.forename: Orsolina
authors.initials: O
authors.lastname: Petillo
authors.affiliation: Institute of Biomedicine and Bioresources; CNR; Naples, Italy; Department of Clinical and Experimental Medicine; Second University of Naples; Naples, Italy.
authors.forename: Mariarosa A B
authors.initials: MA
authors.lastname: Melone
authors.affiliation: Institute of Genetics and Biophysics "Adriano Buzzati-Traverso"; CNR; Naples, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Grimaldi
authors.affiliation: UOC of Neurosurgery; AORN of Caserta; Caserta, Italy.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Bellotti
authors.affiliation: UOC of Neurosurgery; AO "Rummo"; Benevento, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Torelli
authors.affiliation: Neurosurgery Division; PO "Umberto I"; Nocera Inferiore, Italy.
authors.forename: Maria Serena
authors.initials: MS
authors.lastname: De' Santi
authors.affiliation: PO "Santa Maria di Loreto Mare"; Napoli, Italy; UO of Neurosurgery; AOU "San Giovanni di Dio e Ruggi di Aragona"; Salerno, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Cantatore
authors.affiliation: Department of Endocrinology and Clinical and Molecular Oncology; University "Federico II"; Naples, Italy.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Marinelli
authors.affiliation: Institute of Biomedicine and Bioresources; CNR; Naples, Italy; Department of Experimental Medicine; Second University of Naples; Naples, Italy.
authors.forename: Umberto
authors.initials: U
authors.lastname: Galderisi
authors.affiliation: Institute of Biomedicine and Bioresources; CNR; Naples, Italy.
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Peluso
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D001932:Brain Neoplasms / Q000201:enzymology* / Q000235:genetics; D002334:Carnitine O-Palmitoyltransferase / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D018392:Genomic Imprinting; D005909:Glioblastoma / Q000201:enzymology* / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D012097:Repressor Proteins; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D005227:Fatty Acids; D012097:Repressor Proteins; D014157:Transcription Factors; C531063:ZFP57 protein, human; C577474:CPT1A protein, human; C542591:CPT1B protein, human; D002334:Carnitine O-Palmitoyltransferase
keywords: glioblastoma; imprinting; lipid metabolism; mitochondria