title: Zinc is essential for the transcription function of Nrf2 in human renal tubule cells in vitro and mouse kidney in vivo under the diabetic condition.
abstract: Increasing evidence from human and laboratory studies showed the effect of zinc (Zn) on diabetic complications. Nuclear factor-erythroid 2-related factor 2 (Nrf2) plays important role in the prevention of oxidative damage. This study was to define whether Zn statues (deficiency or supplement) affect the Nrf2 expression and function, and also affect the damage severity of human renal tubular (HK11) cells exposed to high glucose (HG) with palmitate (Pal) and kidney of diabetic mice induced by multiple low-dose streptozotocins. For Zn deficiency diabetic mice were treated with Zn chelator PTEN at 5 mg/kg bw daily for 4 months. Results showed that HG/Pal significantly increased the expression of pro-fibrotic mediators, connective tissue growth factor and PAI-1, in HK11 cells, which was exacerbated by TPEN that depleted intracellular free Zn and decreased Nrf2 expression and transcription. Zn supplement prevented the effects of TPEN and also increased Akt and GSK-3β phosphorylation with a decrease in Nrf2 nuclear exporter, Fyn. All these effects of Zn were abolished by Akt inhibitor. Therefore, Zn up-regulates Nrf2 function via activating Akt-mediated inhibition of Fyn function. Treatment of diabetic mice with TPEN decreased renal Zn level and Nrf2 expression and transcription, with an exacerbation of renal oxidative damage, inflammation and fibrosis. These results suggest the essentiality of Zn for Nrf2 expression and transcription function.
authors.affiliation: Department of Nephrology, The Second Hospital of Jilin University, Changchun, China; Kosair Children's Hospital Research Institute, and Departments of Pediatrics and Pharmacology and Toxicology, University of Louisville, Louisville, KY, USA; Department of Nephrology, Jilin Province People's Hospital, Changchun, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.forename: Wenpeng
authors.initials: W
authors.lastname: Cui
authors.forename: Yi
authors.initials: Y
authors.lastname: Tan
authors.forename: Ping
authors.initials: P
authors.lastname: Luo
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Qu
authors.forename: Lining
authors.initials: L
authors.lastname: Miao
authors.forename: Lu
authors.initials: L
authors.lastname: Cai
mesh_terms: D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D055513:Connective Tissue Growth Factor / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000235:genetics* / Q000473:pathology*; D005355:Fibrosis; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D007249:Inflammation / Q000473:pathology; D007684:Kidney Tubules / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D051379:Mice; D008954:Models, Biological; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D010168:Palmitates / Q000494:pharmacology; D017395:Plasminogen Activator Inhibitor 1 / Q000378:metabolism; D021381:Protein Transport / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051076:Proto-Oncogene Proteins c-fyn / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects; D015032:Zinc / Q000172:deficiency / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D051267:NF-E2-Related Factor 2; D010168:Palmitates; D017395:Plasminogen Activator Inhibitor 1; D055513:Connective Tissue Growth Factor; D051076:Proto-Oncogene Proteins c-fyn; D051057:Proto-Oncogene Proteins c-akt; D005947:Glucose; D015032:Zinc
keywords: Akt phosphorylation; Fyn; Nrf2; TPEN; diabetic nephropathy; zinc chelation