title: [Development of the quantitative immunochromatography tests for somatic deasease markers detection].
abstract: The purpose of work was creation of quantative immunochromatographic tests (ICT) for measuring concentrations of the marker substance associated with somatic diseases: immunoglobulin E (IgE), C-reactive protein (CRP) and fibrin D-dimer in blood serum (plasma), which is carried out with the help of videodigital analyzers of domestic development "Reflecom" and "Zondaj". ICT were designed in sandwich-format, using colloid gold and monoclonal antibodies. It is shown, that calibration curves received with the help of ICTand devices of videodigital registration, are well approximated by exponential dependence Y = Aexp(-x/B)+y0, where Y--device readings, x--analytes concentration, A, B, y0--constants. Samples of blood serum (plasma), under investigation received in conditions of a hospital from patients. Correlation with electrochemiluminescent immunoassay, enzyme linked immunoassay, latex-agglutinations assay and immunochromatographic method was observed. Sensitivity of the developed tests was 7,5 IU/ML for IgE, 5,7 ng/ml for CRP, 500 ng/ml for fibrin D-dimer. The developed analytical complex- videodigital analyzer "Zondaj" and ICT for quantitative measure of concentration IgE, fibrin D-dimer and CRP- can be successfully applied in laboratory practice and clinical laboratory researches alongside with actual immunochemistry methods.
authors.forename: S I
authors.initials: SI
authors.lastname: Tret'iakov
authors.forename: L A
authors.initials: LA
authors.lastname: Basharova
authors.forename: S P
authors.initials: SP
authors.lastname: Iarkov
authors.forename: A A
authors.initials: AA
authors.lastname: Titiov
authors.forename: E N
authors.initials: EN
authors.lastname: Khramov
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D002097:C-Reactive Protein / Q000032:analysis; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis; D015203:Reproducibility of Results
chemical_list: D015415:Biomarkers; D007073:Immunoglobulin E; D002097:C-Reactive Protein