title: Prevalence of human papillomavirus and Epstein-Barr virus DNA in Chinese children with tonsillar and/or adenoidal hypertrophy.
abstract: Tonsillar and adenoidal hypertrophy are prevalent otolaryngologic disorders in children, but their pathogenesis is largely unknown. The presence of human papillomavirus (HPV) and Epstein-Barr virus (EBV) DNA in 146 tonsil and/or adenoid tissue specimens from 104 Chinese children with tonsillar and/or adenoidal hypertrophy were screened using flow-through hybridization gene-chip technology and real-time fluorescence-based quantitative PCR. Then, the relationships between the prevalence of the viruses and other clinical characteristics of tonsillar and/or adenoidal hypertrophy were analyzed. No patient had HPV DNA. EBV DNA was detected in 19/42 (45.2%) tonsil tissues and 72/104 (69.2%) adenoid tissue specimens (P < 0.05). EBV DNA was not related to the patients' age, gender, disease course, or nationality, but children positive for EBV were less likely to snore; 14/15 (93.3%) patients who did not snore and 59/89 (66.3%) patients who snored were EBV positive. EBV DNA, but not HPV DNA was detected in Chinese children with tonsillar and/or adenoidal hypertrophy. Adenoid tissues might more susceptible than tonsil tissues to EBV infection. In addition, EBV infection did not aggravate snoring in patients with tonsillar and/or adenoidal hypertrophy.
authors.affiliation: Postgraduate Education College, Ningxia Medical University, Yinchuan, China.
authors.forename: Xiao-cheng
authors.initials: XC
authors.lastname: Xue
authors.forename: Xiao-ping
authors.initials: XP
authors.lastname: Chen
authors.forename: Wen-hao
authors.initials: WH
authors.lastname: Yao
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Guang-bin
authors.initials: GB
authors.lastname: Sun
authors.forename: Xue-jun
authors.initials: XJ
authors.lastname: Tan
mesh_terms: D000234:Adenoids / Q000473:pathology* / Q000821:virology; D044466:Asian People; D002648:Child; D002675:Child, Preschool; D002681:China; D004247:DNA; D020031:Epstein-Barr Virus Infections / Q000453:epidemiology* / Q000821:virology; D005260:Female; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D006984:Hypertrophy / Q000821:virology*; D008297:Male; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000821:virology; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D004247:DNA
keywords: Epstein-Barr virus; adenoidal hypertrophy; human papillomavirus; tonsillar hypertrophy