title: Transcriptome analysis of silkworm, Bombyx mori, during early response to Beauveria bassiana challenges.
abstract: Host-pathogen interactions are complex processes and it is a central challenge to reveal these interactions. Fungal infection of silkworm, Bombyx mori, may induce a variety of responsive reaction. However, little is known about the molecular mechanism of silkworm immune response against the fungal infection. To obtain an overview of the interaction between silkworm and an entomopathogenic fungus Beauveria bassiana, Digital Gene Expression profiling, a tag based high-throughput transcriptome sequencing method, was employed to screen and identify differentially expressed genes (DEGs, FDR ≤ 0.001, ∣log2ratio∣ ≥ 1) of silkworm larvae during early response against B. bassiana infection. Total 1430 DEGs including 960 up-regulated and 470 down-regulated ones were identified, of which 627 DEGs can be classified into GO categories by Gene Ontology (GO) analysis. KEGG pathways analysis of these DEGs suggested that many biological processes, such as defense and response, signal transduction, phagocytosis, regulation of gene expression, RNA splicing, biosynthesis and metabolism, protein transport etc. were involved in the interaction between the silkworm and B. bassiana. A number of differentially expressed fungal genes were also identified by mapping the sequencing tags to B. bassiana genome. These results provided new insights to the molecular mechanism of silkworm immune response to B. bassiana infection.
authors.affiliation: Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, Jiangsu, China.
authors.forename: Chengxiang
authors.initials: C
authors.lastname: Hou
authors.affiliation: Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, Jiangsu, China.
authors.forename: Guangxing
authors.initials: G
authors.lastname: Qin
authors.affiliation: Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, Jiangsu, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.affiliation: Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Geng
authors.affiliation: Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Gao
authors.affiliation: Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, China.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Pan
authors.affiliation: Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, Jiangsu, China.
authors.forename: Heying
authors.initials: H
authors.lastname: Qian
authors.affiliation: Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, Jiangsu, China.
authors.forename: Xijie
authors.initials: X
authors.lastname: Guo
mesh_terms: D000818:Animals; D052982:Beauveria; D012831:Bombyx / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology*; D016000:Cluster Analysis; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions / Q000235:genetics*; D015203:Reproducibility of Results; D015398:Signal Transduction; D013997:Time Factors; D059467:Transcriptome