title: Analysis of additives in dairy products by liquid chromatography coupled to quadrupole-orbitrap mass spectrometry.
abstract: A new method combining QuEChERS with ultrahigh-performance liquid chromatography and electrospray ionization quadrupole Orbitrap high-resolution mass spectrometry (UHPLC/ESI Q-Orbitrap) was developed for the highly accurate and sensitive screening of 43 antioxidants, preservatives and synthetic sweeteners in dairy products. Response surface methodology was employed to optimize a quick, easy, cheap, effective, rugged, and safe (QuEChERS) sample preparation method for the determination of 42 different analytes in dairy products for the first time. After optimization, the maximum predicted recovery was 99.33% rate for aspartame under the optimized conditions of 10 mL acetionitrile, 1.52 g sodium acetate, 410 mg PSA and 404 mgC18. For the matrices studied, the recovery rates of the other 42 compounds ranged from 89.4% to 108.2%, with coefficient of variation <6.4%. UHPLC/ESI Q-Orbitrap Mass full scan mode acquired full MS data was used to identify and quantify additives, and data-dependent scan mode obtained fragment ion spectra for confirmation. The mass accuracy typically obtained is routinely better than 1.5ppm, and only need to calibrate once a week. The 43 compounds behave dynamic in the range 0.001-1000 μg kg(-1) concentration, with correlation coefficient >0.999. The limits of detection for the analytes are in the range 0.0001-3.6 μg kg(-1). This method has been successfully applied on screening of antioxidants, preservatives and synthetic sweeteners in commercial dairy product samples, and it is very useful for fast screening of different food additives.
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China; College of chemistry and chemical Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Jia
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Ling
authors.affiliation: Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100026, China.
authors.forename: Yuanhui
authors.initials: Y
authors.lastname: Lin
authors.affiliation: ThermoFisher Scientific, 355 River Oaks Parkway, San Jose, California 95134, USA.
authors.forename: James
authors.initials: J
authors.lastname: Chang
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China. Electronic address: xgangchu@163.com.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Chu
mesh_terms: D000975:Antioxidants / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003611:Dairy Products / Q000032:analysis*; D005503:Food Additives / Q000032:analysis* / Q000302:isolation & purification; D005520:Food Preservatives / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013549:Sweetening Agents / Q000032:analysis
chemical_list: D000975:Antioxidants; D005503:Food Additives; D005520:Food Preservatives; D013549:Sweetening Agents
keywords: Q-Orbitrap; QuEChERS; antioxidants; dairy products.; preservatives; synthetic sweeteners