title: A targeting peptide improves adenovirus-mediated transduction of a glioblastoma cell line.
abstract: The progress of the application of adenovirus in cancer gene therapy is hindered by the lack of expression of native adenovirus receptor on a variety of cancer types. Hence, strategies are needed to retarget the adenoviral vector to non-native cellular surface receptors. In the present study, a new peptide SWDIAWPPLKVP, capable of selectively targeting a human glioblastoma cell line A172, was identified by direct biopanning of phage-display peptide libraries. The binding activity of the phage displaying SWDIAWPPLKVP peptide in A172 was more than 10-fold higher than that of the control phage. We then inserted the selected peptide SWDIAWPPLKVP into adenoviral hexon protein, and observed that the modified Ad5 had increased infectivity in A172 cells, compared with that in control cell lines. These findings demonstrated that a peptide acquired through phage display can mediate cell-specific Ad retargeting when inserted into Ad hexon, suggesting an approach for targeting adenoviral infection to specific cancer cells.
authors.affiliation: Laboratory of Gene Therapy, Department of Biochemistry, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710062, P.R. China.
authors.forename: Dongyang
authors.initials: D
authors.lastname: Wang
authors.affiliation: Laboratory of Gene Therapy, Department of Biochemistry, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710062, P.R. China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Li
authors.affiliation: Laboratory of Gene Therapy, Department of Biochemistry, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710062, P.R. China.
authors.forename: Hang
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611, USA.
authors.forename: Qinwen
authors.initials: Q
authors.lastname: Mao
authors.affiliation: Laboratory of Gene Therapy, Department of Biochemistry, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710062, P.R. China.
authors.forename: Haibin
authors.initials: H
authors.lastname: Xia
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D001932:Brain Neoplasms / Q000235:genetics / Q000628:therapy; D045744:Cell Line, Tumor; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D005909:Glioblastoma / Q000235:genetics* / Q000628:therapy*; D057809:HEK293 Cells; D006801:Humans; D019151:Peptide Library; D011991:Receptors, Virus / Q000096:biosynthesis; D014161:Transduction, Genetic / Q000379:methods*; D053585:Virus Attachment
chemical_list: D019151:Peptide Library; D011991:Receptors, Virus; C085301:adenovirus receptor