title: Isolation and HPLC method development of azafrin from Alectra parasitica var. chitrakutensis.
abstract: This study was undertaken to isolate and quantify azafrin in Alectra parasitica (Scrophulariaceae) rhizomes. A simple method for the isolation of carotenoid, azafrin, involves solvent extraction of the dried rhizome powder using a single solvent and further purification by recrystallisation. The structure of the compound was elucidated and confirmed by thin-layer chromatography, infrared spectroscopy, mass spectroscopy and nuclear magnetic resonance spectral analysis. A specific and rapid reversed-phase high-performance liquid chromatography (HPLC) method was developed for the analysis of azafrin. The method was validated for accuracy, precision, linearity and specificity. Validation revealed that the method is specific, accurate, precise, reliable and reproducible. The proposed HPLC method can be used for the identification and quantitative analysis of azafrin in A. parasitica rhizomes.
authors.affiliation: a Medicinal Natural Products Research Laboratory (MNPRL), Department of Pharmaceutical Sciences and Technology , Institute of Chemical Technology , Nathalal Parekh Marg, Matunga (E), Mumbai 400 019 , India.
authors.forename: Poonam
authors.initials: P
authors.lastname: Agrawal
authors.forename: Kirti
authors.initials: K
authors.lastname: Laddha
authors.forename: Ashok
authors.initials: A
authors.lastname: Tiwari
mesh_terms: D002338:Carotenoids / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer; D019906:Nuclear Magnetic Resonance, Biomolecular; D010936:Plant Extracts / Q000737:chemistry; D027343:Rhizome / Q000737:chemistry; D028083:Scrophulariaceae / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D002338:Carotenoids; C047580:azafrin
keywords: Alectra parasitica; HPLC; azafrin; isolation