title: Fast magnetic isolation of simple sequence repeat markers in microfluidic channels.
abstract: Simple sequence repeat (SSR) markers are widely used for genome mapping, genetic diversity characterization and medical diagnosis. The fast isolation by AFLP of sequence containing repeats (FIASCO) is a powerful method for SSR marker isolation, but it is laborious, costly, and time consuming and requires multiple rounds of washing. Here, we report a superparamagnetic bead (SPMB)-based FIASCO method in a magnetic field controllable microfluidic chip (MFCM-Chip). This method dramatically reduces the assay time by 4.25-fold and reduces the quantity of magnetic beads and probes by 10-fold through the magnetic capture of (AG)n-containing fragments from Herba Leonuri, followed by washing and eluting on a microchip. The feasibility of this method was further evaluated by PCR and sequencing, and the results showed that the proportion of fragments containing SSRs was 89%, confirming that this platform is a fast and efficient method for SSR marker isolation. This cost-effective platform will make the powerful FIASCO technique more accessible for routine use with a wide variety of materials.
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, and State Key Laboratory of Virology, Wuhan University, Wuhan 430072, PR China. ljli@whu.edu.cn zlzhang@whu.edu.cn.
authors.forename: Shibin
authors.initials: S
authors.lastname: He
authors.forename: Xu
authors.initials: X
authors.lastname: Yu
authors.forename: Xiangwu
authors.initials: X
authors.lastname: Wang
authors.forename: Junjun
authors.initials: J
authors.lastname: Tan
authors.forename: Shihan
authors.initials: S
authors.lastname: Yan
authors.forename: Pu
authors.initials: P
authors.lastname: Wang
authors.forename: Bi-Hai
authors.initials: BH
authors.lastname: Huang
authors.forename: Zhi-Ling
authors.initials: ZL
authors.lastname: Zhang
authors.forename: Lijia
authors.initials: L
authors.lastname: Li
mesh_terms: D003362:Cost-Benefit Analysis; D005819:Genetic Markers / Q000235:genetics; D060526:Magnetic Fields; D059346:Magnets / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000191:economics / Q000379:methods*; D018895:Microsatellite Repeats / Q000235:genetics*; D019809:Streptavidin / Q000737:chemistry; D013997:Time Factors
chemical_list: D005819:Genetic Markers; D019809:Streptavidin