title: Self-assembled core-shell micelles from peptide-b-polymer molecular chimeras towards structure-activity relationships.
abstract: The aim of this contribution is to design, produce and characterize size-tuneable core-shell micelles from amphiphilic Tat-b-poly(trimethylene carbonate) (Tat-b-PTMC) molecular chimeras, and to explore their biological properties. Because the extensive characterization of nanomaterials is a pre-requisite to understand and rationalize their ensuing properties, we present a detailed description of Tat-b-PTMC micelles thanks to light scattering, AFM imaging and small angle neutron scattering analyses. In vitro, Tat-b-PTMC micelles were found to be rapidly and efficiently internalized by HeLa cells, with cellular uptake kinetics being mostly related to Tat peptide content and, to a lesser extent, to nanoparticle size. We also demonstrated that, after a first membrane-binding step, Tat-b-PTMC micelles were taken up by cells via an energy-dependent endocytotic process.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Drappier
authors.forename: Hugo
authors.initials: H
authors.lastname: Oliveira
authors.forename: Olivier
authors.initials: O
authors.lastname: Sandre
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Ibarboure
authors.forename: Sophie
authors.initials: S
authors.lastname: Combet
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Garanger
authors.forename: Sébastien
authors.initials: S
authors.lastname: Lecommandoux
mesh_terms: D001704:Biopolymers / Q000737:chemistry*; D008823:Micelles; D010455:Peptides / Q000737:chemistry*; D013329:Structure-Activity Relationship
chemical_list: D001704:Biopolymers; D008823:Micelles; D010455:Peptides