title: Clinical and treatment characteristics determining therapeutic outcome in patients undergoing autologous non-cultured outer root sheath hair follicle cell suspension for treatment of stable vitiligo.
abstract: BACKGROUND
  Autologous non-cultured outer root sheath hair follicle cell suspension (NCORSHFS) is a recently described novel cellular graft technique for the treatment of stable vitiligo. There is lack of data about various factors determining the repigmentation rate in vitiligo patients undergoing this novel surgical therapy.
  
  
  OBJECTIVE
  To study the clinical characteristics and treatment variables determining therapeutic outcome in patients of stable vitiligo undergoing NCORSHFS.
  
  
  METHODS
  Non-cultured outer root sheath hair follicle cell suspension was prepared from anagen hairs extracted from the occipital area. The number of melanocytes and hair follicle stem cells (HFSC) in the suspension was quantified by staining with anti-HMB45 and anti-CD200 antibody, respectively. In all patients, a 2 mm punch skin biopsy was taken from one of the vitiligo patch to be treated prior to surgery for assessment of histomorphological features. Post surgery patients were followed up at regular intervals for 24 weeks.
  
  
  RESULTS
  Thirty patients (21 females, 9 males) with a clinical diagnosis of stable vitiligo, with a total of 60 target lesions were included in this study. The mean age of the study population was 21.10 ± 5.64 years. The number of melanocytes (P = 0.04) and HFSC (P = 0.01) transplanted were significantly higher among patients achieving optimum repigmentation (>75% repigmentation). There was a strong correlation between repigmentation at 24 week and number of melanocytes and HFSC transplanted. Number of HFSC transplanted and absence of dermal inflammation were significant predictors of achieving optimum repigmentation.
  
  
  CONCLUSIONS
  The number of melanocytes and HFSC transplanted and absence of dermal inflammation were important determents of optimal repigmentation in patients undergoing NCORSHFS for treatment of stable vitiligo. Hence, refining the technique of NCORSHFS on the basis of these factors would help in achieving better surgical outcomes.
authors.affiliation: Department of Dermatology, Venereology and Leprology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
authors.forename: K
authors.initials: K
authors.lastname: Vinay
authors.forename: S
authors.initials: S
authors.lastname: Dogra
authors.forename: D
authors.initials: D
authors.lastname: Parsad
authors.forename: A J
authors.initials: AJ
authors.lastname: Kanwar
authors.forename: R
authors.initials: R
authors.lastname: Kumar
authors.forename: R W
authors.initials: RW
authors.lastname: Minz
authors.forename: U N
authors.initials: UN
authors.lastname: Saikia
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D005500:Follow-Up Studies; D018859:Hair Follicle / Q000166:cytology / Q000637:transplantation*; D006801:Humans; D008297:Male; D008544:Melanocytes / Q000637:transplantation*; D011446:Prospective Studies; D012880:Skin Pigmentation; D033581:Stem Cell Transplantation; D014182:Transplantation, Autologous / Q000379:methods; D016896:Treatment Outcome; D014820:Vitiligo / Q000601:surgery*; D055815:Young Adult