title: Effect of haemostatic agent application on the shear bond strength of contemporary/multi-mode adhesive systems.
abstract: OBJECTIVE
  The aim was to determine the effect of blood contamination and haemostatic agent application on the shear bond strength (SBS) of different adhesives to dentin.
  
  
  METHODS
  Seventy-two extracted human molars were used in this study. Teeth were mounted acrylic in moulds. Mesial and distal surfaces were removed to obtain flat dentin surfaces (n=144) and grinded with a 600 grit sandpaper. The samples were randomly divided into three main groups (N=48) based on the adhesive system and application procedures. GROUP 1: No contamination (control), GROUP 2: Blood contamination, GROUP 3: Blood Contamination+Haemostatic agent application. Each group was further divided into four subgroups: SUBGROUP I: Single Bond 2 (etch-and-rinse) Subgroup II: Clearfil SE Bond (two-step self-etch) Subgroup III: Single Bond Universal (multimode, etch-and-rinse) subgroup IV: Single Bond Universal (multimode, all-in-one self-etch) (n=12). Resin cylinders (Filtek Z550) were bonded to the dentin surfaces according to the manufacturers' instructions. A shear load was applied to the specimens using universal testing machine at a cross-head speed of 0.5 mm/min until failure. Data were analyzed statistically (p<0.05).
  
  
  RESULTS
  Statistically significant differences were observed between no contamination (control) and blood + haemostatic agent contamination groups for both Single Bond 2 (etch-and-rinse) and Single Bond Universal (multimode, all-in-one self-etch) (p<0.05). When the adhesive systems were compared to each other, no statistically significant differences were found in all contamination groups and control group for the mean SBS values (p>0.05).
  
  
  CONCLUSIONS
  When blood contamination is inevitable two step self- etching adhesive systems may be the choice of adhesive system in terms of shear bond strength.
authors.affiliation: Department of Restorative Dentistry, Başkent University, School of Dentistry, 11. sokak No:26 Bahçelievler 06490, Ankara, Turkey, Tel: 90-312-2151336; e-mail: duygukayaoglu@yahoo.com.
authors.forename: Duygu
authors.initials: D
authors.lastname: Tuncer
authors.forename: Saffet
authors.initials: S
authors.lastname: Başaran
authors.forename: Derya Merve
authors.initials: DM
authors.lastname: Halaçoglu
authors.forename: Kıvanc
authors.initials: K
authors.lastname: Yamanel
authors.forename: Çigdem
authors.initials: Ç
authors.lastname: Çelik
authors.forename: Neslihan
authors.initials: N
authors.lastname: Arhun