title: Isolation and characterization of extracellular polysaccharide Thelebolan produced by a newly isolated psychrophilic Antarctic fungus Thelebolus.
abstract: The present investigation is on a newly isolated psychrophilic Antarctic filamentous Ascomycetous fungus that has been identified as Thelebolus sp. and given the designation of Thelebolus sp. IITKGP-BT12. The culture was primarily identified through morphological studies, and was further confirmed by 18S rRNA sequencing (GenBank Accession No. KC191572), which revealed its close relatedness with Thelebolus microsporus. The exopolysaccharide (EPS) produced (1.94 g L(-1)) by the fungus was isolated, purified and characterized as glucan having an average molecular mass of 5×10(5)Da. The structure of EPS was determined by gas chromatography with tandem mass spectrometry (GC-MS/MS), Fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance (NMR) studies ((1)H, (13)C and HSQC). NMR analysis indicated the presence of (1→3)-linked β-d-glucan backbone with (1→6)-linked branches of β-d-glucopyranosyl units. Antiproliferative activity of EPS was demonstrated in B16-F0 cells, with IC50 of 275.42 μg m L(-1). Flow cytometry analysis and DNA fragmentation studies revealed that the cytotoxic action of the EPS mediated apoptosis in cancer cells. This is the first ever report on bioactive EPS thelebolan from Thelebolus sp.
authors.affiliation: Plant Biotechnology Laboratory, Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, West Bengal, India.
authors.forename: Sourav K
authors.initials: SK
authors.lastname: Mukhopadhyay
authors.affiliation: Plant Biotechnology Laboratory, Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, West Bengal, India.
authors.forename: Soumya
authors.initials: S
authors.lastname: Chatterjee
authors.affiliation: Plant Biotechnology Laboratory, Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, West Bengal, India.
authors.forename: Samiran S
authors.initials: SS
authors.lastname: Gauri
authors.affiliation: Plant Biotechnology Laboratory, Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, West Bengal, India.
authors.forename: Shibendu S
authors.initials: SS
authors.lastname: Das
authors.affiliation: Plant Biotechnology Laboratory, Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, West Bengal, India.
authors.forename: Abheepsa
authors.initials: A
authors.lastname: Mishra
authors.affiliation: Plant Biotechnology Laboratory, Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, West Bengal, India.
authors.forename: Moumita
authors.initials: M
authors.lastname: Patra
authors.affiliation: Plant Biotechnology Laboratory, Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, West Bengal, India.
authors.forename: Ananta K
authors.initials: AK
authors.lastname: Ghosh
authors.affiliation: Plant Biotechnology Laboratory, Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, West Bengal, India.
authors.forename: Amit K
authors.initials: AK
authors.lastname: Das
authors.affiliation: National Centre for Antarctic and Ocean Research, Goa 403804, India.
authors.forename: Shiv M
authors.initials: SM
authors.lastname: Singh
authors.affiliation: Plant Biotechnology Laboratory, Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, West Bengal, India. Electronic address: satyahari01@yahoo.com.
authors.forename: Satyahari
authors.initials: S
authors.lastname: Dey
mesh_terms: D000818:Animals; D000864:Antarctic Regions; D017209:Apoptosis; D001203:Ascomycota / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005110:Extracellular Space / Q000737:chemistry; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D051379:Mice
chemical_list: D062610:Fungal Polysaccharides
keywords: Antarctic; Antiproliferative; Exopolysaccharide; Glucan; Psychrophiles; Thelebolus