title: TTC26/DYF13 is an intraflagellar transport protein required for transport of motility-related proteins into flagella.
abstract: Cilia/flagella are assembled and maintained by the process of intraflagellar transport (IFT), a highly conserved mechanism involving more than 20 IFT proteins. However, the functions of individual IFT proteins are mostly unclear. To help address this issue, we focused on a putative IFT protein TTC26/DYF13. Using live imaging and biochemical approaches we show that TTC26/DYF13 is an IFT complex B protein in mammalian cells and Chlamydomonas reinhardtii. Knockdown of TTC26/DYF13 in zebrafish embryos or mutation of TTC26/DYF13 in C. reinhardtii, produced short cilia with abnormal motility. Surprisingly, IFT particle assembly and speed were normal in dyf13 mutant flagella, unlike in other IFT complex B mutants. Proteomic and biochemical analyses indicated a particular set of proteins involved in motility was specifically depleted in the dyf13 mutant. These results support the concept that different IFT proteins are responsible for different cargo subsets, providing a possible explanation for the complexity of the IFT machinery. DOI: http://dx.doi.org/10.7554/eLife.01566.001.
authors.affiliation: Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, United States.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ishikawa
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ide
authors.forename: Toshiki
authors.initials: T
authors.lastname: Yagi
authors.forename: Xue
authors.initials: X
authors.lastname: Jiang
authors.forename: Masafumi
authors.initials: M
authors.lastname: Hirono
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sasaki
authors.forename: Haruaki
authors.initials: H
authors.lastname: Yanagisawa
authors.forename: Kimberly A
authors.initials: KA
authors.lastname: Wemmer
authors.forename: Didier Yr
authors.initials: DY
authors.lastname: Stainier
authors.forename: Hongmin
authors.initials: H
authors.lastname: Qin
authors.forename: Ritsu
authors.initials: R
authors.lastname: Kamiya
authors.forename: Wallace F
authors.initials: WF
authors.lastname: Marshall
mesh_terms: D020418:Algal Proteins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002465:Cell Movement; D016825:Chlamydomonas reinhardtii / Q000235:genetics / Q000378:metabolism*; D002923:Cilia / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005407:Flagella / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D005838:Genotype; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009154:Mutation; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D014162:Transfection; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D020418:Algal Proteins; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D010940:Plant Proteins; C000599077:Ttc26 protein, mouse; C577010:Ttc26 protein, zebrafish; D029961:Zebrafish Proteins
keywords: Chlamydomonas; axoneme; dynein; flagella