title: Isolation of neural progenitor cells from the human adult subventricular zone based on expression of the cell surface marker CD271.
abstract: Neural progenitor cells (NPCs) in the subventricular zone (SVZ) hold promise for future therapy for neurodegenerative disorders, because the stimulation of adult neurogenesis could potentially restore the function of degenerating neurons and glia. To obtain more knowledge on these NPCs, we developed a method to specifically isolate NPCs from postmortem adult human brains based on the expression of the specific human adult neural stem/progenitor cell marker glial fibrillary acidic protein δ (GFAPδ). An extensive immunophenotyping analysis for cell surface markers resulted in the observation that CD271 was limited to the SVZ-derived GFAPδ-positive cells. CD271(+) cells developed into neurospheres and could be differentiated into astrocytes, neurons, and oligodendrocytes. We are the first to show that a pure population of NPCs can be isolated from the adult human SVZ, which is highly instrumental for developing future therapies based on stimulating endogenous SVZ neurogenesis.
authors.affiliation: Department of Astrocyte Biology and Neurodegeneration, The Netherlands Institute for Neuroscience, an Institute of the Royal Netherlands Academy of Sciences, Amsterdam, The Netherlands; Department of Neurosurgery, McKnight Brain Institute, University of Florida, Gainesville, Florida, USA; Department of Neuropathology, Academic Medical Center, Amsterdam, The Netherlands; Swammerdam Institute for Life Sciences, Center for Neuroscience, University of Amsterdam, Amsterdam, The Netherlands; Department of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center, Utrecht, The Netherlands.
authors.forename: Miriam E
authors.initials: ME
authors.lastname: van Strien
authors.forename: Jacqueline A
authors.initials: JA
authors.lastname: Sluijs
authors.forename: Brent A
authors.initials: BA
authors.lastname: Reynolds
authors.forename: Dennis A
authors.initials: DA
authors.lastname: Steindler
authors.forename: Eleonora
authors.initials: E
authors.lastname: Aronica
authors.forename: Elly M
authors.initials: EM
authors.lastname: Hol
mesh_terms: D000328:Adult; D000943:Antigens, Differentiation / Q000096:biosynthesis*; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism; D001921:Brain / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation; D002469:Cell Separation; D005260:Female; D015870:Gene Expression; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis; D006801:Humans; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism; D055495:Neurogenesis / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism; D017475:Receptors, Nerve Growth Factor / Q000096:biosynthesis*
chemical_list: D000943:Antigens, Differentiation; D005904:Glial Fibrillary Acidic Protein; C484065:NGFR protein, human; D009419:Nerve Tissue Proteins; D017475:Receptors, Nerve Growth Factor
keywords: Glial fibrillary acidic protein delta; Human adult neural progenitor cells; Neurodegenerative disorders; Specific isolation; Subventricular zone