title: The effects of low-level diode laser irradiation on differentiation, antigenic profile, and phagocytic capacity of osteoblast-like cells (MG-63).
abstract: Previous in vivo and in vitro studies have reported that low-level diode laser therapy induces a biostimulatory effect, such as cell proliferation. The aim of the present study was to evaluate whether the laser irradiation of osteoblast-like cells (MG-63) can modify alkaline phosphatase activity (ALP), antigenic profile, and phagocytic capacity. The MG-63 cell line was exposed to diode laser (ezLase) of 940 nm at 1-1.5 W/cm(2) and 3-4 J. ALP was evaluated by a spectrophotometric technique and antigenic expression analysis (CD 54, CD80, CD86, HLA-DR), and phagocytic activity was analyzed by flow cytometry. At 24 h, the treated groups showed an increased ALP, and the highest increase versus controls (P = 0.002) was at the dose of 1 W/cm(2) and 3 J; this modulation of the antigenic profile translated into a reduced expression of CD54, CD86, and HLA-DR and a slightly decreased phagocytic capacity with respect to the nonirradiated control group at the different intensities and fluencies assayed. These results demonstrate that laser therapy can exert a biostimulatory effect on osteoblastic cells at different levels, which may be clinically useful in the regeneration of bone tissue.
authors.affiliation: BIO277 Group, Faculty of Health Sciences, University of Granada, Granada, 18071, Spain.
authors.forename: Rosa
authors.initials: R
authors.lastname: Medina-Huertas
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Manzano-Moreno
authors.forename: Elvira
authors.initials: E
authors.lastname: De Luna-Bertos
authors.forename: Javier
authors.initials: J
authors.lastname: Ramos-Torrecillas
authors.forename: Olga
authors.initials: O
authors.lastname: García-Martínez
authors.forename: Concepción
authors.initials: C
authors.lastname: Ruiz
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D015703:Antigens, CD / Q000378:metabolism; D002454:Cell Differentiation / Q000528:radiation effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D057899:Cytophagocytosis / Q000528:radiation effects*; D006801:Humans; D054023:Lasers, Semiconductor; D028022:Low-Level Light Therapy; D010006:Osteoblasts / Q000502:physiology* / Q000528:radiation effects
chemical_list: D015703:Antigens, CD; D000469:Alkaline Phosphatase