title: Promising results of cranberry in the prevention of oral Candida biofilms.
abstract: In the context of dental caries prevention by natural foodstuff sources, antifungal and antibiofilm activities of dry commercial extracts of cranberry fruit (Vaccinium macrocarpon Aiton) and two other red fruits (Vaccinium myrtillus L. and Malpighia punicifolia L.) were assessed on Candida albicans and Candida glabrata yeasts. When added to the culture medium, the cranberry extract displayed a significant anti-adhesion activity against Candida spp. when used at low concentrations. In addition, the pretreatment of surfaces with this extract induced an anti-adhesion activity mainly against C. glabrata yeasts and an antibiofilm activity against C. albicans. This activity was dependent on concentration, species, and strain. A phytochemical investigation bioguided by anti-adhesion tests against the two Candida species was carried out on crude cranberry juice to determine the active fractions. Three subfractions enriched in proanthocyanidins showed an anti-adhesion activity at low concentrations. This study investigated for the first time the interest of crude extracts of cranberry and cranberry juice fractions to prevent biofilms of C. glabrata. It highlighted the potency of consuming this fruit and using it as a source of anti-adhesion agents.
authors.affiliation: UMR CNRS 7267 Laboratory of Ecology and Biology of Interactions, Faculty of Medicine and Pharmacy, Poitiers, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Girardot
authors.forename: Amandine
authors.initials: A
authors.lastname: Guerineau
authors.forename: Leslie
authors.initials: L
authors.lastname: Boudesocque
authors.forename: Damien
authors.initials: D
authors.lastname: Costa
authors.forename: Laurent
authors.initials: L
authors.lastname: Bazinet
authors.forename: Cécile
authors.initials: C
authors.lastname: Enguehard-Gueiffier
authors.forename: Christine
authors.initials: C
authors.lastname: Imbert
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects*; D002175:Candida / Q000187:drug effects* / Q000502:physiology*; D002180:Candidiasis, Oral / Q000517:prevention & control*; D005591:Chemical Fractionation; D022001:Focal Adhesions / Q000187:drug effects; D005638:Fruit / Q000737:chemistry; D006801:Humans; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D013499:Surface Properties; D029799:Vaccinium macrocarpon / Q000737:chemistry*
chemical_list: D000935:Antifungal Agents; D010936:Plant Extracts
keywords: functional food; oral diseases; oral yeasts; phytochemical study; polyphenols; red berries