title: Adaptive optics without altering visual perception.
abstract: Adaptive optics combined with visual psychophysics creates the potential to study the relationship between visual function and the retina at the cellular scale. This potential is hampered, however, by visual interference from the wavefront-sensing beacon used during correction. For example, we have previously shown that even a dim, visible beacon can alter stimulus perception (Hofer et al., 2012). Here we describe a simple strategy employing a longer wavelength (980nm) beacon that, in conjunction with appropriate restriction on timing and placement, allowed us to perform psychophysics when dark adapted without altering visual perception. The method was verified by comparing detection and color appearance of foveally presented small spot stimuli with and without the wavefront beacon present in 5 subjects. As an important caution, we found that significant perceptual interference can occur even with a subliminal beacon when additional measures are not taken to limit exposure. Consequently, the lack of perceptual interference should be verified for a given system, and not assumed based on invisibility of the beacon.
authors.affiliation: University of Houston College of Optometry, 4901 Calhoun Rd., Houston, TX 77204, USA. Electronic address: darren.koenig@mcphs.edu.
authors.forename: D E
authors.initials: DE
authors.lastname: Koenig
authors.affiliation: University of Houston College of Optometry, 4901 Calhoun Rd., Houston, TX 77204, USA. Electronic address: nate@camerafinder.org.
authors.forename: N W
authors.initials: NW
authors.lastname: Hart
authors.affiliation: University of Houston College of Optometry, 4901 Calhoun Rd., Houston, TX 77204, USA. Electronic address: hhofer@optometry.uh.edu.
authors.forename: H J
authors.initials: HJ
authors.lastname: Hofer
mesh_terms: D000221:Adaptation, Ocular / Q000502:physiology*; D000328:Adult; D003118:Color Perception / Q000502:physiology*; D003119:Color Perception Tests / Q000379:methods*; D055253:Color Vision / Q000502:physiology*; D006801:Humans; D055095:Optics and Photonics / Q000379:methods*; D011601:Psychophysics; D012684:Sensory Thresholds / Q000502:physiology
keywords: Adaptive optics; Color appearance; Perceptual interference; Psychophysics; Wavefront beacon