title: Expression of SRC suppressed C kinase substrate in rat neural tissues during inflammation.
abstract: Src-suppressed C kinase substrate (SSeCKS), an in vivo and in vitro protein kinase C substrate, is a major lipopolysaccharide (LPS) response protein which markedly upregulated in several organs, including brain, lung, heart, kidney etc., indicating a possible role of SSeCKS in inflammatory process. However, the expression and biological function of SSeCKS during neuronal inflammation remains to be elucidated, so we established an inflammatory model injected with LPS to investigate the gene expression patterns of SSeCKS in neural tissues by using TaqMan quantitative real-time PCR and immunohistochemistry in rat. Real-time PCR showed that LPS stimulated the expression of SSeCKS mRNA in a dose- and time-dependent manner in sciatic nerves, spinal cords and dorsal root ganglions. Immunohistochemistry showed that SSeCKS colocalized with nerve fibers in sciatic nerve after LPS administration, but there was no colocalization between SSeCKS and Schwann cells. In addition, SSeCKS colocalized with neurons which existed in dorsal root ganglions and spinal cords. These findings indicated that SSeCKS might play some important roles in sciatic nerve fibers and neurons in spinal cords and dorsal root ganglions after LPS injection.
authors.affiliation: Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong University, 19 Qi-xiu Road, Nantong, 226001, Jiangsu, China.
authors.forename: Meijuan
authors.initials: M
authors.lastname: Yan
authors.forename: Jianmei
authors.initials: J
authors.lastname: Zhao
authors.forename: Shunxin
authors.initials: S
authors.lastname: Zhu
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Shao
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Heng
authors.initials: H
authors.lastname: Gao
authors.forename: Dengfu
authors.initials: D
authors.lastname: Yao
mesh_terms: D054758:A Kinase Anchor Proteins / Q000096:biosynthesis*; D000818:Animals; D018797:Cell Cycle Proteins / Q000096:biosynthesis*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D015971:Gene Expression Regulation, Enzymologic; D007249:Inflammation / Q000201:enzymology / Q000473:pathology; D008297:Male; D009474:Neurons / Q000201:enzymology* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000201:enzymology* / Q000473:pathology; D013116:Spinal Cord / Q000201:enzymology / Q000473:pathology
chemical_list: D054758:A Kinase Anchor Proteins; C102361:Akap12 protein, rat; D018797:Cell Cycle Proteins