title: Synergistic effect of nanosecond pulsed electric field combined with low-dose of pingyangmycin on salivary adenoid cystic carcinoma.
abstract: Adenoid cystic carcinoma (ACC) is one of the most common malignant neoplasms in salivary glands. To evaluate the therapeutic effects of nanosecond pulsed electric field (nsPEF) combined with pingyangmycin (PYM) on salivary gland adenoid cystic carcinoma (SACC), ACC high metastatic cell line (SACC-LM) and low metastatic cell line (SACC‑83) were tested by CCK-8 assay, cell clonogenic assay, flow cytometry and Transwell assay. Extracellular matrix metalloproteinase inducer (EMMPRIN) expression was tested by western blotting to verify the synergistic mechanism of nsPEF and PYM. The results showed that nsPEF inhibited the cell proliferation of both cell lines, and the inhibitory effect was strongly associated with time and electrical field strength. Moreover, PYM combined with nsPEF may enhance the suppression effect significantly, even at a very low dose (0.01 µg/ml). The synergistic effects may contribute to the downregulation of EMMPRIN expression resulting from the application of nsPEF. For SACC, nsPEF combined with chemotherapy agents may be a valuable strategy not only to improve the treatment effect and prognosis, but also to reduce the side-effects of chemotherapy.
authors.affiliation: Department of General Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qi
authors.affiliation: Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, P.R. China.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Guo
authors.affiliation: College of Engineering, Peking University, Beijing 100871, P.R. China.
authors.forename: Shan
authors.initials: S
authors.lastname: Wu
authors.affiliation: Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, P.R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Su
authors.affiliation: Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing 100081, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of General Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Pan
authors.affiliation: Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, P.R. China.
authors.forename: Jue
authors.initials: J
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D051926:Basigin / Q000096:biosynthesis; D001761:Bleomycin / Q000031:analogs & derivatives* / Q000494:pharmacology; D003528:Carcinoma, Adenoid Cystic / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015536:Down-Regulation; D006801:Humans; D009361:Neoplasm Invasiveness; D061208:Pulsed Radiofrequency Treatment / Q000379:methods*; D012468:Salivary Gland Neoplasms / Q000473:pathology / Q000628:therapy*; D012469:Salivary Glands / Q000473:pathology
chemical_list: D001761:Bleomycin; D051926:Basigin; C025703:bleomycetin