title: Fluorescence turn-on sensing of protein based on mannose functionalized perylene bisimides and its fluorescence imaging.
abstract: A new water-soluble glycocluster based on perylene bisimides PBI-12-Man has been designed and synthesized, and its specific and selective binding property with Concanavalin A (Con A) has been investigated by fluorescence spectroscopy and circular dichroism (CD) spectroscopy, which showed strong binding affinity for Con A with the binding constant of 8.2×10(5)M(-1) for monomeric mannose unit, two orders of magnitude higher than the corresponding monosaccharide ligand. Most interestingly, a fluorescence enhancement of PBI-12-Man was observed upon binding with Con A because of deaggregation of the self-assembly of PBI-12-Man induced by carbohydrate-protein interaction, and the further study of the fluorescence enhancement with macrophage cells showed that PBI-12-Man as a biocompatible agent had fluorescence imaging of the surface mannose receptor of the cells. Such fluorescence turn-on sensing of protein based on carbohydrate-protein interactions would facilitate the development of new protein-specific fluorescent probe for diagnosis and molecular imaging under live cell conditions.
authors.affiliation: Key Laboratory of Chemical Biology of Hebei Province, College of Chemistry and Environmental Science, Hebei University, Baoding, PR China; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Baoding, PR China. Electronic address: kerangwang@hbu.edu.cn.
authors.forename: Ke-Rang
authors.initials: KR
authors.lastname: Wang
authors.affiliation: Department of Immunology, School of Basic Medical Science, Hebei University, Baoding, PR China.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: An
authors.affiliation: Department of Immunology, School of Basic Medical Science, Hebei University, Baoding, PR China.
authors.forename: Rui-Xue
authors.initials: RX
authors.lastname: Rong
authors.affiliation: Department of Immunology, School of Basic Medical Science, Hebei University, Baoding, PR China.
authors.forename: Zhi-Ran
authors.initials: ZR
authors.lastname: Cao
authors.affiliation: Key Laboratory of Chemical Biology of Hebei Province, College of Chemistry and Environmental Science, Hebei University, Baoding, PR China; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Baoding, PR China. Electronic address: lixl@hbu.cn.
authors.forename: Xiao-Liu
authors.initials: XL
authors.lastname: Li
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003208:Concanavalin A / Q000032:analysis / Q000737:chemistry*; D007094:Imides / Q000737:chemistry; D008264:Macrophages / Q000737:chemistry* / Q000166:cytology; D008358:Mannose / Q000737:chemistry*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D010569:Perylene / Q000031:analogs & derivatives*; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D007094:Imides; D003208:Concanavalin A; D010569:Perylene; D008358:Mannose
keywords: Biocompatibility; Fluorescence imaging; Fluorescence turn-on sensing; Glycocluster; Perylene bisimide