title: Application of the 3'-noncoding region of poliovirus RNA for cell-based regulation of mRNA stability: implication for biotechnological applications.
abstract: Enrichment of production yield of therapeutic proteins in mammalian cell cultures by modulation of the mRNA stability of the target protein to increase its in vivo half-life is a new strategy in biotechnological applications. The present article describes one of the most novel approaches to modulate mRNA stability by application of 3'-noncoding region (3'NCR) from RNA viral genome in the expression constructs. Our data indicated that although utilizing the 3'NCR sequence form poliovirus (PV-3'NCR) downstream of the target gene might generally stabilize the secondary structure of RNA, it influenced the mRNA stability (and thereby the amount of protein production) in a cell type and time-dependent manner, thus indicating a central role of mRNA-stabilizing binding sites/cellular factors in this process. Our data might be of interest to the biotechnology community to improve recombinant protein production in mammalian cell cultures and RNA-based therapy/vaccination approaches.
authors.affiliation: Virology Department, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Mahsa
authors.initials: M
authors.lastname: Rasekhian
authors.forename: Farzin
authors.initials: F
authors.lastname: Roohvand
authors.forename: Ladan
authors.initials: L
authors.lastname: Teimoori-Toolabi
authors.forename: Safieh
authors.initials: S
authors.lastname: Amini
authors.forename: Kayhan
authors.initials: K
authors.lastname: Azadmanesh
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D001709:Biotechnology; D009690:Nucleic Acid Conformation; D017955:Poliovirus / Q000235:genetics; D012313:RNA / Q000737:chemistry* / Q000235:genetics; D020871:RNA Stability / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry; D013329:Structure-Activity Relationship
chemical_list: D011994:Recombinant Proteins; D012313:RNA
keywords: 3′NCR; RNA secondary structure; post transcriptional RNA processing; protein expression