title: Masitinib mesylate does not enhance sensitivity to radiation in three feline injection-site sarcoma cell lines under normal growth conditions.
abstract: Masitinib, a selective tyrosine kinase inhibitor, was investigated as a radiosensitizer in three primary feline injection-site sarcoma (ISS) cell lines. Sensitivity to masitinib was previously assessed via cell growth inhibition assays with mean IC50 values of 5.5-8.6μM. Clonogenic assays were performed to determine the effect of masitinib and radiation on cell survival. Single dose radiation (0-12Gy) experiments were carried out under normal growth conditions in control ISS cells and in cells incubated with 1 or 6μM masitinib for 72h prior to irradiation. Radiation administered either alone or in combination with masitinib induced a dose-dependent reduction in clonogenic survival. Survival from the combined masitinib and radiation treatment was not significantly different from that of radiation alone. Results suggest that masitinib does not directly enhance ISS cell radiosensitivity under normal in vitro conditions, although this does not preclude the utility of further investigations to assess sensitization properties under altered conditions.
authors.affiliation: Department of Veterinary Biosciences and Diagnostic Imaging, College of Veterinary Medicine, University of Georgia, Athens, GA, USA; Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA, USA. Electronic address: mmturek@wisc.edu.
authors.forename: M
authors.initials: M
authors.lastname: Turek
authors.affiliation: Department of Veterinary Biosciences and Diagnostic Imaging, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
authors.forename: R
authors.initials: R
authors.lastname: Gogal
authors.affiliation: Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
authors.forename: C
authors.initials: C
authors.lastname: Saba
authors.affiliation: Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
authors.forename: M L
authors.initials: ML
authors.lastname: Vandenplas
authors.affiliation: Department of Veterinary Biosciences and Diagnostic Imaging, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
authors.forename: J
authors.initials: J
authors.lastname: Hill
authors.affiliation: Department of Veterinary Biosciences and Diagnostic Imaging, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
authors.forename: B
authors.initials: B
authors.lastname: Feldhausser
authors.affiliation: Department of Veterinary Biosciences and Diagnostic Imaging, College of Veterinary Medicine, University of Georgia, Athens, GA, USA; Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
authors.forename: J
authors.initials: J
authors.lastname: Lawrence
mesh_terms: D000818:Animals; D001549:Benzamides; D002371:Cat Diseases / Q000532:radiotherapy*; D002415:Cats; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D010880:Piperidines; D047428:Protein Kinase Inhibitors / Q000494:pharmacology* / Q000627:therapeutic use; D011725:Pyridines; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology* / Q000627:therapeutic use; D012509:Sarcoma / Q000532:radiotherapy / Q000662:veterinary*; D013844:Thiazoles / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D001549:Benzamides; D010880:Piperidines; D047428:Protein Kinase Inhibitors; D011725:Pyridines; D011838:Radiation-Sensitizing Agents; D013844:Thiazoles; C526575:masitinib
keywords: Cat; Clonogenic; Masitinib; Radiation; Sarcoma; Tyrosine kinase inhibitor