title: Use of the anti-Prelog stereospecific alcohol dehydrogenase from Leifsonia and Pseudomonas for producing chiral alcohols.
abstract: The asymmetric reduction of ketones is one of the most promising processes for producing chiral alcohols. However, dehydrogenases or reductases that can catalyze the reduction of ketones to give anti-Prelog chiral alcohols have been limited to some NADP(+)/NADPH-dependent enzymes. Recently, we reported a novel NAD(+)/NADH-dependent alcohol dehydrogenase (ADH) from Leifsonia sp. and Pseudomonas ADH homologs from soil metagenomes. Moreover, we have established an efficient hydrogen-transfer bioreduction process with 2-propanol as a hydrogen donor using Leifsonia ADH. This review focuses on the recent development of novel ADHs for producing industrially useful anti-Prelog chiral alcohols from various ketones.
authors.affiliation: Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama, 939-0398, Japan, nbito@pu-toyama.ac.jp.
authors.forename: Nobuya
authors.initials: N
authors.lastname: Itoh
mesh_terms: D019840:2-Propanol / Q000378:metabolism; D039903:Actinobacteria / Q000201:enzymology*; D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D000438:Alcohols / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods / Q000639:trends; D006859:Hydrogen / Q000378:metabolism; D007659:Ketones / Q000378:metabolism; D010084:Oxidation-Reduction; D011549:Pseudomonas / Q000201:enzymology*
chemical_list: D000438:Alcohols; D007659:Ketones; D006859:Hydrogen; D000426:Alcohol Dehydrogenase; D019840:2-Propanol