title: Ultrasound targeted microbubble destruction for novel dual targeting of HSP72 and HSC70 in prostate cancer.
abstract: The aim was to determine whether ultrasound targeted microbubble destruction (UTMD) promotes dual targeting of HSP72 and HSC70 for therapy of castration-resistant prostate cancer (CRPC), to improve the specific and efficient delivery of siRNA, to induce tumor cell specific apoptosis, and to find new therapeutic targets specific of CRPC.VCaP cells were transfected with siRNA oligonucleotides. HSP70, HSP90 and cleaved caspase-3 expression were determined by real-time quantitative polymerase chain reaction and Western blotting. Apoptosis and transfection efficiency were assessed by flow cytometry. Cell viability assays were used to evaluate safety. We found HSP72, HSC70 and HSP90 expression to be absent or weak in normal prostate epithelial cells (RWPE-1), but uniformly strong in prostate cancerous cells (VCaP). UTMD combined with dual targeting of HSP72 and HSC70 siRNA improve the efficiency of transfection, cell uptake of siRNA, downregulation of HSP70 and HSP90 expression in VCaP cells at the mRNA and protein level, and induction of extensive tumor-specific apoptosis. Cell counting kit-8 assays showed decreased cellular viability in the HSP72/HSC70-siRNA silenced group. These results suggest that the combination of UTMD with dual targeting HSP70 therapy for PCa may be most efficacious, providng a novel, reliable, non-invasive, safe targeted approach to improve the specific and efficient delivery of siRNA, and achieve maximal effects.
authors.affiliation: Department of Ultrasound, Shanghai First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China E-mail : du_lf@163.com.
authors.forename: Hang-Hui
authors.initials: HH
authors.lastname: Wang
authors.forename: Yi-Xin
authors.initials: YX
authors.lastname: Song
authors.forename: Min
authors.initials: M
authors.lastname: Bai
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Jin
authors.forename: Ji-Ying
authors.initials: JY
authors.lastname: Gu
authors.forename: Yi-Jin
authors.initials: YJ
authors.lastname: Su
authors.forename: Long
authors.initials: L
authors.lastname: Liu
authors.forename: Chao
authors.initials: C
authors.lastname: Jia
authors.forename: Lian-Fang
authors.initials: LF
authors.lastname: Du
mesh_terms: D017209:Apoptosis / Q000235:genetics; D053148:Caspase 3 / Q000096:biosynthesis / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D015536:Down-Regulation; D015316:Genetic Therapy; D050883:HSC70 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D050884:HSP72 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D018841:HSP90 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008297:Male; D045423:Microbubbles; D064129:Prostatic Neoplasms, Castration-Resistant / Q000000981:diagnostic imaging / Q000235:genetics* / Q000628:therapy; D034622:RNA Interference; D034741:RNA, Small Interfering; D014162:Transfection; D014463:Ultrasonography
chemical_list: D050883:HSC70 Heat-Shock Proteins; D050884:HSP72 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D034741:RNA, Small Interfering; D053148:Caspase 3