title: Lack of association between TNF-alpha promoter polymorphism and prostate carcinoma susceptibility in eastern Croatian population.
abstract: A single nuclear polymorphisms (SNPs) in the promoter region of the tumor necrosis factor-alpha (TNF-alpha) gene are involved in regulation of expression levels of TNF-alpha and therefore are associated with oncogenesis of several cancers. Aim of our study was to investigate the effect of G--->A polymorphism at -308 position in the promoter region of the TNF-alpha gene on prostate cancer (CalphaP) susceptibility in a subset of patients from Eastern Croatia. Study population consisted of 240 patients (120 with CalphaP, 120 controls). They were genotyped for TNF-alpha G-308A polymorphism using real-time PCR (LightCycler Instrument, Roche Diagnostics) and melting curve analysis method. X(2) test was used to compare distribution of TNF-alpha polymorphism genotypes between patients and control group. Relative risk was estimated by the odds ratio (OR). There was no significant statistical difference (X(2)=0.000, DF=1, p=1, OR=1, 95%CI=0.5537-1.8059) between patients and control group. Besides, data of CalphaP patients were stratified according to pathohistological diagnosis (PHD) by Gleason score and groups were compared according to TNF-alpha genotypes. Also, all patients and CalphaP patients were grouped according to prostate volume (V) into three groups: V<50 mL, V50-100 mL, V>100 mL. These groups were also compared according to TNF-alpha genotypes. There were no significant statistical differences between any of groups. Our findings suggest that TNF-alpha -308 SNP is not associated with CalphaP in Eastern Croatia population.
authors.affiliation: University Josip Juraj Strossmayer, University Hospital Centre Osijek, Department of Clinical Laboratory Diagnostics, Osijek, Croatia. phorvat.vesna@gmail.com
authors.forename: Vesna
authors.initials: V
authors.lastname: Horvat
authors.affiliation: University Josip Juraj Strossmayer, University Hospital Centre Osijek, Clinic of Urology, Osijek, Croatia.
authors.forename: Bojan
authors.initials: B
authors.lastname: Sudarević
authors.affiliation: University Josip Juraj Strossmayer, University Hospital Centre Osijek, Clinic of Urology, Osijek, Croatia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Cosić
authors.affiliation: University Josip Juraj Strossmayer, University Hospital Centre Osijek, Department of Anesthesiology, Reanimatology and Intensive Care Unit, Osijek, Croatia.
authors.forename: Nevenka
authors.initials: N
authors.lastname: Milicević
authors.affiliation: University Josip Juraj Strossmayer, University Hospital Centre Osijek, Department of Anesthesiology, Reanimatology and Intensive Care Unit, Osijek, Croatia.
authors.forename: Silvana
authors.initials: S
authors.lastname: Bosnjak
authors.affiliation: University Josip Juraj Strossmayer, University Hospital Centre Osijek, Department of Clinical Laboratory Diagnostics, Osijek, Croatia.
authors.forename: Sanja
authors.initials: S
authors.lastname: Mandić
authors.affiliation: University Josip Juraj Strossmayer, University Hospital Centre Osijek, Clinical Institute of Nuclear Medicine and Radiation Protection, Department of Molecular Diagnostics and Tissue Typing, Osijek, Croatia.
authors.forename: Saska
authors.initials: S
authors.lastname: Marczi
authors.affiliation: University Josip Juraj Strossmayer, University Hospital Centre Osijek, Clinic of Urology, Osijek, Croatia.
authors.forename: Dalibor
authors.initials: D
authors.lastname: Simunović
authors.affiliation: University Josip Juraj Strossmayer, University Hospital Centre Osijek, Clinic of Urology, Osijek, Croatia.
authors.forename: Josip
authors.initials: J
authors.lastname: Galić
mesh_terms: D017523:Croatia; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D011471:Prostatic Neoplasms / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D014409:Tumor Necrosis Factor-alpha