title: Modular plasmonic nanocarriers for efficient and targeted delivery of cancer-therapeutic siRNA.
abstract: We have combined a versatile and powerful route to deliver nucleic acids with peptide-based cell-specific targeting. siRNA targeting the polo-like kinase gene is in clinical trials for cancer treatment, and here we deliver this RNA selectively to cancer cells displaying the neuropilin-1 epitope using gold nanoshells. Release of the siRNA from the nanoparticles results from irradiation with a pulsed near-infrared laser, which also provides efficient endosomal escape within the cell. As a result, our approach requires 10-fold less material than standard nucleic acid transduction materials and is significantly more efficient than other particle-based methods. We also describe a particle-nucleic acid design that does not rely on modified RNA, thereby making the preparation of these materials more efficient and much less expensive. These improvements, when combined with control over when and where the siRNA is released, could provide the basis for diverse cell biological studies.
authors.affiliation: Department of Chemistry and Biochemistry, University of California , Santa Barbara, California 93106, United States.
authors.forename: Xiao
authors.initials: X
authors.lastname: Huang
authors.forename: Alessia
authors.initials: A
authors.lastname: Pallaoro
authors.forename: Gary B
authors.initials: GB
authors.lastname: Braun
authors.forename: Demosthenes P
authors.initials: DP
authors.lastname: Morales
authors.forename: Maria O
authors.initials: MO
authors.lastname: Ogunyankin
authors.forename: Joseph
authors.initials: J
authors.lastname: Zasadzinski
authors.forename: Norbert O
authors.initials: NO
authors.lastname: Reich
mesh_terms: D000595:Amino Acid Sequence; D002460:Cell Line; D045744:Cell Line, Tumor; D003692:Delayed-Action Preparations / Q000737:chemistry* / Q000378:metabolism; D016503:Drug Delivery Systems; D011992:Endosomes / Q000378:metabolism; D018014:Gene Transfer Techniques; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007834:Lasers; D008297:Male; D053769:Nanocapsules / Q000737:chemistry*; D039942:Neuropilin-1 / Q000378:metabolism; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D011467:Prostate / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000628:therapy; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics / Q000378:metabolism
chemical_list: D003692:Delayed-Action Preparations; D053769:Nanocapsules; D010455:Peptides; D034741:RNA, Small Interfering; D039942:Neuropilin-1; D006046:Gold