title: 14-3-3 Binding to Cyclin Y contributes to cyclin Y/CDK14 association.
abstract: Cyclin Y is a highly conserved cyclin among eumetazoans, yet its function and regulation are poorly understood. To search for Cyclin Y-interacting proteins, we screened a yeast two-hybrid library using human Cyclin Y (CCNY) as a bait and identified the following interactors: CDK14 and four members of the 14-3-3 family (ε, β, η, τ). The interaction between CCNY and 14-3-3 proteins was confirmed both in vitro and in vivo. The results showed that Ser-100 and Ser-326 residues in CCNY were crucial for 14-3-3 binding. Interestingly, binding of CCNY to 14-3-3 significantly enhanced the association between CCNY and CDK14. Our findings may add a new layer of regulation of CCNY binding to its kinase partner.
authors.affiliation: Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Li
authors.forename: Mei
authors.initials: M
authors.lastname: Jiang
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Wang
authors.forename: Jiangye
authors.initials: J
authors.lastname: Chen
mesh_terms: D048948:14-3-3 Proteins / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D018844:Cyclin-Dependent Kinases / Q000737:chemistry / Q000378:metabolism*; D016213:Cyclins / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D012694:Serine / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D048948:14-3-3 Proteins; C543319:CCNY protein, human; D016213:Cyclins; D012694:Serine; C515076:CDK14 protein, human; D018844:Cyclin-Dependent Kinases
keywords: 14-3-3 binding motif; 14-3-3 protein; cyclin Y