title: In vitro iron absorption of α-lactalbumin hydrolysate-iron and β-lactoglobulin hydrolysate-iron complexes.
abstract: To study the feasibility of promoting iron absorption by peptides derived from α-lactalbumin and β-lactoglobulin, the present work examined the transport of iron across Caco-2 monolayer cell as in vitro model. Caco-2 cells were seeded in bicameral chambers with α-lactalbumin hydrolysate-Fe (α-LAH-Fe) complex and β-lactoglobulin hydrolysate-Fe (β-LGH-Fe) complex, α-LAH and iron mixture, β-LGH and iron mixture, FeSO4 and ascorbic acid mixture, and FeSO4. In addition, the cytotoxicity of α-LAH-Fe and β-LGH-Fe complexes were measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The iron absorption and ferritin content were assessed using the coupled in vitro digestion/Caco-2 cell model. Results support that peptide-iron complexes can promote ferritin formation and it is possible to apply β-LGH-Fe complexes as iron-fortified supplements with high iron absorbability.
authors.affiliation: Key Laboratory of Functional Dairy of Beijing and Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Functional Dairy of Beijing and Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
authors.forename: T
authors.initials: T
authors.lastname: Ai
authors.affiliation: Key Laboratory of Functional Dairy of Beijing and Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
authors.forename: X L
authors.initials: XL
authors.lastname: Meng
authors.affiliation: Key Laboratory of Functional Dairy of Beijing and Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
authors.forename: J
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Functional Dairy of Beijing and Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. Electronic address: maoxueying@cau.edu.cn.
authors.forename: X Y
authors.initials: XY
authors.lastname: Mao
mesh_terms: D001692:Biological Transport; D018938:Caco-2 Cells; D005293:Ferritins / Q000378:metabolism*; D006801:Humans; D007501:Iron; D007768:Lactalbumin / Q000378:metabolism*; D007782:Lactoglobulins / Q000378:metabolism*; D011492:Protein Hydrolysates / Q000378:metabolism*
chemical_list: D007782:Lactoglobulins; D011492:Protein Hydrolysates; D005293:Ferritins; D007768:Lactalbumin; D007501:Iron
keywords: Caco-2 cells; ferritin; iron absorption; α-lactalbumin hydrolysate-iron complex; β-lactoglobulin hydrolysate-iron complex