title: Cytoprotection of methionine on hyperthermia-induced damage in bovine mammary epithelial cells.
abstract: The protective effects of methionine against hyperthermia-induced damage in bovine mammary epithelial cells (BMEC) were studied. We have investigated the effects of methionine on proliferation, antioxidant activity, and apoptosis of the mammary epithelial cells of dairy cow after heat treatment. The structure of BMEC membrane was damaged by hyperthermia. Methionine (30 and 60 mg/L) efficiently increased cell viability and attenuated morphological damages in hyperthermia-treated BMEC. It significantly reduced lactate dehydrogenase leakage and malondialdehyde formation, whereas superoxide dismutase activity increased significantly. It also increased cell survival and decreased early apoptosis. Methionine therefore is cytoprotective on hyperthermia-induced damage in BMEC by increasing intracellular antioxidant levels and decreasing lipid peroxidation.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Mu
authors.forename: Guang-Huan
authors.initials: GH
authors.lastname: Kong
authors.forename: Zhao-Yu
authors.initials: ZY
authors.lastname: Han
authors.forename: Hui-Xia
authors.initials: HX
authors.lastname: Li
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis; D002417:Cattle; D048430:Cell Shape; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D019610:Cytoprotection; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology*; D005260:Female; D006979:Hyperthermia, Induced; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000166:cytology*; D008715:Methionine / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D008715:Methionine; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase
keywords: bovine mammary epithelial cells; hyperthermia; methionine; oxidative damage