title: Induction of TGF-β and IL-10 production in dendritic cells using astilbin to inhibit dextran sulfate sodium-induced colitis.
abstract: Astilbin, a major bioactive compound from Rhizoma smilacis glabrae, has been reported to possess anti-inflammatory properties. Our study first evaluated astilbin on dextran sulfate sodium (DSS)-induced acute colitis in mice. By intraperitoneal injection of astilbin, the severity of colitis was attenuated, and the serum levels of IL-10 and TGF-β were increased. Using flow cytometry, a higher number of IL-10(+) dendritic cells (DCs) and TGF-β(+) DCs and a lower number of CD86(+) DCs, IL-12 p40(+) DCs, and IL-1β(+) DCs were detected in the spleen of mice with colitis after astilbin treatment. The administration of astilbin also resulted in the upregulation of CD103(+) expression in colonic DCs. In a coculture system, murine bone marrow-derived DCs pretreated with astilbin resulted in an enhanced production of CD4(+)CD25(+)Foxp3(+) T cells. The results of this study show that astilbin could be a candidate drug for inflammatory bowel disease by mediating the regulatory functions of DCs.
authors.affiliation: Department of Gastroenterology, The Second Clinical College of Yangzhou University, 45 Taizhou Road, Yangzhou, Jiangsu 225001, China. Electronic address: yanbingding66@126.com.
authors.forename: Yanbing
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Department of Gastroenterology, The Second Clinical College of Yangzhou University, 45 Taizhou Road, Yangzhou, Jiangsu 225001, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Department of Gastroenterology, The Second Clinical College of Yangzhou University, 45 Taizhou Road, Yangzhou, Jiangsu 225001, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Deng
authors.affiliation: Department of Gastroenterology, The Second Clinical College of Yangzhou University, 45 Taizhou Road, Yangzhou, Jiangsu 225001, China.
authors.forename: Ahui
authors.initials: A
authors.lastname: Qiao
authors.affiliation: Department of Gastroenterology, The Second Clinical College of Yangzhou University, 45 Taizhou Road, Yangzhou, Jiangsu 225001, China.
authors.forename: Keyan
authors.initials: K
authors.lastname: Wu
authors.affiliation: Department of Gastroenterology, The Second Clinical College of Yangzhou University, 45 Taizhou Road, Yangzhou, Jiangsu 225001, China.
authors.forename: Weiming
authors.initials: W
authors.lastname: Xiao
authors.affiliation: Department of Immunology, School of Medicine, Yangzhou University, 11 Huaihai Road, Yangzhou, Jiangsu 225001, China.
authors.forename: Weijuan
authors.initials: W
authors.lastname: Gong
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology*; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology*; D016264:Dextran Sulfate; D044948:Flavonols / Q000008:administration & dosage*; D016753:Interleukin-10 / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016212:Transforming Growth Factor beta / Q000276:immunology*; D016896:Treatment Outcome
chemical_list: D000893:Anti-Inflammatory Agents; D044948:Flavonols; C556062:IL10 protein, mouse; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; C099069:astilbin; D016264:Dextran Sulfate
keywords: Astilbin; Colitis; Dendritic cells