title: Recombinant Leishmania infantum heat shock protein 83 for the serodiagnosis of cutaneous, mucosal, and visceral leishmaniases.
abstract: Routine serological diagnoses for leishmaniases, except in visceral cases, are performed using whole-parasite antigens. We used enzyme-linked immunosorbent assay (ELISA) to evaluate the performance of Leishmania infantum rHsp83 compared with L. major-like total promastigote antigen in the diagnosis of cutaneous (CL), mucosal (ML), and visceral leishmaniases (VL). ELISA-rHsp83 was significantly more sensitive than ELISA-L. major-like when considering either CL/ML (P = 0.041) or all leishmaniasis patients (P = 0.013). When samples from other infectious disease patients were evaluated for cross-reactivity, ELISA-rHsp83 was more specific than ELISA-L. major-like, specifically for Chagas disease samples (P < 0.001). We also evaluated the anti-rHsp83 antibody titers months after treatment and observed no significant difference in ML (P = 0.607) or CL (P = 0.205). We recommend ELISA-L. infantum-rHsp83 as a routine confirmatory serological assay for the diagnosis of Leishmania infection because of the high sensitivity, the specificity, and the insignificant cross-reactivity with other infectious diseases.
authors.forename: Beatriz Julieta
authors.initials: BJ
authors.lastname: Celeste
authors.forename: Maria Carmen
authors.initials: MC
authors.lastname: Arroyo Sanchez
authors.forename: Eduardo Milton
authors.initials: EM
authors.lastname: Ramos-Sanchez
authors.forename: Luiz Guilherme M
authors.initials: LGM
authors.lastname: Castro
authors.forename: Francisco Assis
authors.initials: FA
authors.lastname: Lima Costa
authors.forename: Hiro
authors.initials: H
authors.lastname: Goto
mesh_terms: D000913:Antibodies, Protozoan / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology*; D014355:Chagas Disease / Q000175:diagnosis / Q000276:immunology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005500:Follow-Up Studies; D006360:Heat-Shock Proteins / Q000032:analysis*; D006801:Humans; D018314:Leishmania infantum / Q000302:isolation & purification; D007896:Leishmaniasis / Q000175:diagnosis* / Q000276:immunology; D015800:Protozoan Proteins / Q000032:analysis*; D011994:Recombinant Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity; D012698:Serologic Tests
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D006360:Heat-Shock Proteins; C490310:Hsp83 protein, protozoan; D015800:Protozoan Proteins; D011994:Recombinant Proteins