title: Determination of statins by gas chromatography - EI/MRM - Tandem mass spectrometry: fermentation of pine samples with Pleurotus ostreatus.
abstract: Statins were separated and quantified with gas chromatography-mass spectrometry (GC-EI-MS/MS) using total ion monitoring (TIC) and multiple reactions monitoring (MRM). The MRM method in statins determination has a novelty value, since there are no previous studies on their simultaneous analysis in environmental or plant samples. The method development and optimization was challenging due to the physicochemical similarities of the silylated lovastatin, simvastatin, pravastatin, fluvastatin, and atorvastatin. The results showed that the use of MRM decreased their detection and quantification limits by factors of 2-10 compared to that obtained in TIC monitoring. The concentration calibration was made between 247.5ng/L and 9900ng/L. Limits of detection and quantification were between 50ng/L (lovastatin)-500ng/L (pravastatin) and 250ng/L (lovastatin)-1000ng/L (pravastatin), respectively. Based on the MRM results, the wood bark and phloem samples contained lovastatin, lovastatin-lactone, simvastatin, simvastatin-lactone, and pravastatin. Their concentrations were 250-3000μg/L, i.e. 4.2-50mg/kg in phloem and bark. However, they were not detected in fluids made with Pleurotus ostreatus fermentation of wood core.
authors.affiliation: University of Helsinki, Department of Chemistry, A.I. Virtasen aukio 1, P.O. Box 55, FI-00014 University of Helsinki, Finland. Electronic address: helimmsiren@gmail.com.
authors.forename: Heli
authors.initials: H
authors.lastname: Sirén
authors.affiliation: Lappeenranta University of Technology, LUT Chemistry, P.O. Box 20, FI-53851 Lappeenranta, Finland.
authors.forename: Laura
authors.initials: L
authors.lastname: Kaijanen
authors.affiliation: Lappeenranta University of Technology, LUT Chemistry, P.O. Box 20, FI-53851 Lappeenranta, Finland.
authors.forename: Sini
authors.initials: S
authors.lastname: Kaartinen
authors.affiliation: Helsinki Metropolia University of Applied Sciences, Bulevardi 31, FI-00180 Helsinki, Finland.
authors.forename: Monna
authors.initials: M
authors.lastname: Väre
authors.affiliation: Saimaa University of Applied Sciences, Unit of Technology, Skinnarilankatu 36, FI-53850 Lappeenranta, Finland.
authors.forename: Päivi
authors.initials: P
authors.lastname: Riikonen
authors.affiliation: Lappeenranta University of Technology, LUT Chemistry, P.O. Box 20, FI-53851 Lappeenranta, Finland.
authors.forename: Eeva
authors.initials: E
authors.lastname: Jernström
mesh_terms: D002138:Calibration; D005285:Fermentation / Q000502:physiology; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000737:chemistry*; D057230:Limit of Detection; D028223:Pinus / Q000033:anatomy & histology* / Q000737:chemistry* / Q000382:microbiology; D020076:Pleurotus / Q000254:growth & development*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors
keywords: Fermentation; Gas chromatography; MRM method; Pine; Statin (monacolin)