title: Direct detection of single nucleotide polymorphism (SNP) by the TaqMan PCR assay using dried saliva on water-soluble paper and hair-roots, without DNA extraction.
abstract: We have developed a new method for directly using unprocessed biological specimens as templates for the TaqMan assay. DNA extraction and purification had been believed to be required for the assay, but our new method could avoid hindering fluorescence detection, even if the templates were used directly. Saliva was needed to be put on water-soluble paper and dried, and hairs were cut to be about 10 mm long. This method could reduce both the time and effort involved, and also the risk of contamination. It should prove to be very valuable for genetic diagnoses in various fields.
authors.affiliation: School of Pharmaceutical Sciences, Mukogawa Women's University.
authors.forename: Mariko
authors.initials: M
authors.lastname: Hayashida
authors.forename: Tomoko
authors.initials: T
authors.lastname: Ota
authors.forename: Minori
authors.initials: M
authors.lastname: Ishii
authors.forename: Kyoko
authors.initials: K
authors.lastname: Iwao-Koizumi
authors.forename: Shigenori
authors.initials: S
authors.lastname: Murata
authors.forename: Kenji
authors.initials: K
authors.lastname: Kinoshita
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics; D000444:Aldehyde Dehydrogenase / Q000235:genetics; D000071396:Aldehyde Dehydrogenase, Mitochondrial; D004247:DNA / Q000302:isolation & purification*; D005260:Female; D005838:Genotype; D006197:Hair / Q000737:chemistry*; D064368:Healthy Volunteers; D006801:Humans; D010209:Paper; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012463:Saliva / Q000737:chemistry*; D012995:Solubility; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water; D004247:DNA; C489332:ADH1B protein, human; D000426:Alcohol Dehydrogenase; C096127:ALDH2 protein, human; D000444:Aldehyde Dehydrogenase; D000071396:Aldehyde Dehydrogenase, Mitochondrial