title: A second-generation protein-protein interaction network of Helicobacter pylori.
abstract: Helicobacter pylori infections cause gastric ulcers and play a major role in the development of gastric cancer. In 2001, the first protein interactome was published for this species, revealing over 1500 binary protein interactions resulting from 261 yeast two-hybrid screens. Here we roughly double the number of previously published interactions using an ORFeome-based, proteome-wide yeast two-hybrid screening strategy. We identified a total of 1515 protein-protein interactions, of which 1461 are new. The integration of all the interactions reported in H. pylori results in 3004 unique interactions that connect about 70% of its proteome. Excluding interactions of promiscuous proteins we derived from our new data a core network consisting of 908 interactions. We compared our data set to several other bacterial interactomes and experimentally benchmarked the conservation of interactions using 365 protein pairs (interologs) of E. coli of which one third turned out to be conserved in both species.
authors.affiliation: German Cancer Research Center (Deutsches Krebsforschungszentrum), Technologiepark 3, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany;
authors.forename: Roman
authors.initials: R
authors.lastname: Häuser
authors.forename: Arnaud
authors.initials: A
authors.lastname: Ceol
authors.forename: Seesandra V
authors.initials: SV
authors.lastname: Rajagopala
authors.forename: Roberto
authors.initials: R
authors.lastname: Mosca
authors.forename: Gabriella
authors.initials: G
authors.lastname: Siszler
authors.forename: Nadja
authors.initials: N
authors.lastname: Wermke
authors.forename: Patricia
authors.initials: P
authors.lastname: Sikorski
authors.forename: Frank
authors.initials: F
authors.lastname: Schwarz
authors.forename: Matthias
authors.initials: M
authors.lastname: Schick
authors.forename: Stefan
authors.initials: S
authors.lastname: Wuchty
authors.forename: Patrick
authors.initials: P
authors.lastname: Aloy
authors.forename: Peter
authors.initials: P
authors.lastname: Uetz
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000378:metabolism*; D017124:Conserved Sequence; D016480:Helicobacter pylori / Q000378:metabolism*; D016366:Open Reading Frames; D025941:Protein Interaction Mapping / Q000379:methods*; D060066:Protein Interaction Maps; D020543:Proteome / Q000032:analysis; D040901:Proteomics; D020798:Two-Hybrid System Techniques
chemical_list: D001426:Bacterial Proteins; D020543:Proteome