title: In-solution multiplex miRNA detection using DNA-templated silver nanocluster probes.
abstract: MicroRNAs (miRNAs) are small regulatory RNAs (size ∼21nt to ∼25nt) that can be used as biomarkers of disease diagnosis, and efforts have been directed towards the invention of a rapid, simple and sequence-selective detection method for miRNAs. We recently developed a DNA/silver nanoclusters (AgNCs)-based turn-off fluorescence method in the presence of target miRNA. To further advance our method toward multiplex miRNA detection in solution, the design of various fluorescent DNA/AgNCs probes was essential. Therefore, tethering of DNA-12nt scaffolds with 9 different AgNCs emitters to target-sensing DNA sequences was investigated. Interestingly, for the creation of spectrally different DNA/AgNCs probes, not only were the emitters encapsulated in 9 different DNA-12nt scaffolds necessary but the tethered target-sensing DNA sequences are also crucial to tune the fluorescence across the visible to infra-red region. In this study, we obtained three spectrally distinctive emitters of each DNA/AgNCs probes such as green, red, and near-infrared (NIR) fluorescence. Using these DNA/AgNCs probes, we here show a proof of concept for a rapid, one-step, in-solution multiplex miRNA detection method.
authors.affiliation: UNIK Center for Synthetic Biology/Plant Biochemistry Laboratory, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Copenhagen, Denmark. swyang@life.ku.dk mobj@chem.ku.dk.
authors.forename: Pratik
authors.initials: P
authors.lastname: Shah
authors.forename: Peter Waaben
authors.initials: PW
authors.lastname: Thulstrup
authors.forename: Seok Keun
authors.initials: SK
authors.lastname: Cho
authors.forename: Yong-Joo
authors.initials: YJ
authors.lastname: Bhang
authors.forename: Jong Cheol
authors.initials: JC
authors.lastname: Ahn
authors.forename: Suk Won
authors.initials: SW
authors.lastname: Choi
authors.forename: Morten Jannik
authors.initials: MJ
authors.lastname: Bjerrum
authors.forename: Seong Wook
authors.initials: SW
authors.lastname: Yang
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D035683:MicroRNAs / Q000032:analysis*; D015335:Molecular Probes; D049329:Nanostructures; D063347:Native Polyacrylamide Gel Electrophoresis; D012689:Sequence Homology, Nucleic Acid; D012834:Silver / Q000737:chemistry*
chemical_list: D035683:MicroRNAs; D015335:Molecular Probes; D012834:Silver; D004247:DNA