title: Increase in insulin-induced relaxation of consecutive arterial segments toward the periphery: Role of vascular oxidative state.
abstract: BACKGROUND
  The oxidative state has been implicated in the signaling of various vasomotor functions, yet its role regarding the vasomotor action of insulin is less known.
  
  
  OBJECTIVE
  To investigate the insulin-evoked relaxations of consecutive arterial segments of different oxidative state and the role of extracellular signal-regulated kinase (ERK) pathway.
  
  
  RESULTS
  The oxidative state, as assessed by the level of ortho-tyrosine, was higher in the thoracic aorta of rats than in the abdominal aorta, and was the lowest in the femoral artery. The vasomotor function of vessels of same origin was studied using a small-vessel myograph. Insulin-induced relaxations increased toward the periphery (i.e., thoracic < abdominal < femoral). Aortic banding and hydrogen peroxide/aminotriazole increased the oxidative state of the thoracic aorta that was accompanied by ERK activation and decreased relaxation to insulin, and vice versa, acutely lowered oxidative state by superoxide dismutase/catalase improved relaxation. In contrast, insulin-induced relaxation of the femoral artery could be enhanced with a higher oxidative state, and reduced with a lower state.
  
  
  CONCLUSIONS
  Oxidative state of vessels modulates the magnitude of vasomotor responses to insulin, which appears to be mediated via the ERK signaling pathway.
authors.affiliation: 2nd Department of Medicine and Nephrological Center, University of Pécs , Pécs , Hungary.
authors.forename: I A
authors.initials: IA
authors.lastname: Szijártó
authors.forename: G A
authors.initials: GA
authors.lastname: Molnár
authors.forename: E
authors.initials: E
authors.lastname: Mikolás
authors.forename: V
authors.initials: V
authors.lastname: Fisi
authors.forename: B
authors.initials: B
authors.lastname: Laczy
authors.forename: M
authors.initials: M
authors.lastname: Gollasch
authors.forename: A
authors.initials: A
authors.lastname: Koller
authors.forename: I
authors.initials: I
authors.lastname: Wittmann
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000002:abnormalities / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D007328:Insulin / Q000008:administration & dosage / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007328:Insulin; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: antioxidants; aortic banding; insulin; ortho-tyrosine; oxidative state; vascular function