title: Inhibition of matrix metalloproteinase-1 and type-I procollagen expression by phenolic compounds isolated from the leaves of Quercus mongolica in ultraviolet-irradiated human fibroblast cells.
abstract: The aim of this study was to investigate the effect of Quercus mongolica (QM) which induce anti-photoaging process of skin in vitro. Bioassay-guided isolation of 80 % Me2CO extract of the leaves of QM led to the isolation and identification of six known phenolic compounds: pedunculagin (1), (-)-epigallocatechin (2), (+)-catechin (3), quercetin 3-O-(6″-O-galloyl)-β-D-glucopyranoside (4), kaempferol-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside (5) and kaempferol 3-O-(6″-galloyl)-β-D-glucopyranoside (6). The effects of compounds 1-6 on expression of matrix metalloproteinase-1 (MMP-1) and type-I procollagen were further evaluated. Among them, compound 1 showed potent inhibitory effect on MMP-1 and the increased type-I procollagen synthesis in ultraviolet B-induced human fibroblast. These results suggest that pedunculagin, an ellagitannin, is a potential candidate for the prevention and treatment of skin aging.
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, Chung-Ang University, Seoul, 156-756, Republic of Korea.
authors.forename: Han Hyuk
authors.initials: HH
authors.lastname: Kim
authors.forename: Dong Hee
authors.initials: DH
authors.lastname: Kim
authors.forename: Myeong Hwan
authors.initials: MH
authors.lastname: Oh
authors.forename: Kwang Jun
authors.initials: KJ
authors.lastname: Park
authors.forename: Jun Hyeok
authors.initials: JH
authors.lastname: Heo
authors.forename: Min Won
authors.initials: MW
authors.lastname: Lee
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis*; D015536:Down-Regulation / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects*; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism*; D061965:Matrix Metalloproteinase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D015394:Molecular Structure; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors / Q000378:metabolism; D010636:Phenols / Q000302:isolation & purification* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D029963:Quercus / Q000737:chemistry*; D015595:Skin Aging / Q000187:drug effects; D014466:Ultraviolet Rays
chemical_list: D024042:Collagen Type I; D016166:Free Radical Scavengers; D061965:Matrix Metalloproteinase Inhibitors; D010636:Phenols; D010196:Pancreatic Elastase; D020781:Matrix Metalloproteinase 1