title: MiRNA let-7g regulates skeletal myoblast motility via Pinch-2.
abstract: Post-transcriptional regulation of gene expression by RNA-binding proteins and by small non-coding RNAs plays an important role in cell biology. Our previous results show that in murine skeletal myoblasts, the expression of Pinch-2, a focal adhesion remodeling factor that regulates cell motility, is repressed by an RNA-binding protein IMP-2/Igf2bp2. We now show that the expression of Pinch-2 is also regulated by the miRNA let-7g. Let-7g and IMP-2 repress Pinch-2 expression independently of each other. A knock-down of let-7g leads to an increase in Pinch-2 expression, and to a decrease of cell motility, which can be reversed by a simultaneous knock-down of Pinch-2. We conclude that let-7g controls the motility of mouse myoblasts in cell culture by post-transcriptionally regulating the expression of Pinch-2.
authors.affiliation: CNRS FRE 3377, CEA Saclay, F-91191 Gif-sur-Yvette, France; Univ Paris-Sud, FRE 3377, F-91191 Gif-sur-Yvette, France; Department of Biochemistry and Biophysics, University of California, San Francisco, CA, USA.
authors.forename: S
authors.initials: S
authors.lastname: Boudoukha
authors.affiliation: CNRS FRE 3377, CEA Saclay, F-91191 Gif-sur-Yvette, France; Univ Paris-Sud, FRE 3377, F-91191 Gif-sur-Yvette, France.
authors.forename: T
authors.initials: T
authors.lastname: Rivera Vargas
authors.affiliation: Centre de Recherche de Gif, Laboratoire d'Enzymologie et Biochimie Structurales, CNRS UPR3082, Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France.
authors.forename: I
authors.initials: I
authors.lastname: Dang
authors.affiliation: CNRS FRE 3377, CEA Saclay, F-91191 Gif-sur-Yvette, France; Univ Paris-Sud, FRE 3377, F-91191 Gif-sur-Yvette, France.
authors.forename: J
authors.initials: J
authors.lastname: Kropp
authors.affiliation: Inserm U1016, Institut Cochin, INSERM U1016, CNRS 8104, Université Paris Descartes, F-75014 Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Cuvellier
authors.affiliation: Centre de Recherche de Gif, Laboratoire d'Enzymologie et Biochimie Structurales, CNRS UPR3082, Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France.
authors.forename: A
authors.initials: A
authors.lastname: Gautreau
authors.affiliation: CNRS FRE 3377, CEA Saclay, F-91191 Gif-sur-Yvette, France; Univ Paris-Sud, FRE 3377, F-91191 Gif-sur-Yvette, France. Electronic address: anna.polesskaya@cea.fr.
authors.forename: A
authors.initials: A
authors.lastname: Polesskaya
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D060565:Argonaute Proteins / Q000378:metabolism; D001483:Base Sequence; D002460:Cell Line; D002465:Cell Movement; D015870:Gene Expression; D060588:LIM Domain Proteins / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D035683:MicroRNAs / Q000502:physiology*; D008969:Molecular Sequence Data; D032448:Myoblasts, Skeletal / Q000502:physiology*; D034622:RNA Interference; D016601:RNA-Binding Proteins / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C526735:Ago2 protein, mouse; D060565:Argonaute Proteins; C559768:IGF2BP2 protein, mouse; D060588:LIM Domain Proteins; C490234:Lims2 protein, mouse; D008565:Membrane Proteins; D035683:MicroRNAs; D016601:RNA-Binding Proteins; C542687:mirnlet7 microRNA, mouse
keywords: Cell adhesion; Cell motility; Let-7g; PINCH-2; RNA-binding protein; Skeletal muscle; microRNA