title: Genome-wide analysis of regulation of gene expression and H3K9me2 distribution by JIL-1 kinase mediated histone H3S10 phosphorylation in Drosophila.
abstract: In this study we have determined the genome-wide relationship of JIL-1 kinase mediated H3S10 phosphorylation with gene expression and the distribution of the epigenetic H3K9me2 mark. We show in wild-type salivary gland cells that the H3S10ph mark is predominantly enriched at active genes whereas the H3K9me2 mark is largely associated with inactive genes. Comparison of global transcription profiles in salivary glands from wild-type and JIL-1 null mutant larvae revealed that the expression levels of 1539 genes changed at least 2-fold in the mutant and that a substantial number (49%) of these genes were upregulated whereas 51% were downregulated. Furthermore, the results showed that downregulation of genes in the mutant was correlated with higher levels or acquisition of the H3K9me2 mark whereas upregulation of a gene was correlated with loss of or diminished H3K9 dimethylation. These results are compatible with a model where gene expression levels are modulated by the levels of the H3K9me2 mark independent of the state of the H3S10ph mark, which is not required for either transcription or gene activation to occur. Rather, H3S10 phosphorylation functions to indirectly maintain active transcription by counteracting H3K9 dimethylation and gene silencing.
authors.affiliation: Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA.
authors.forename: Weili
authors.initials: W
authors.lastname: Cai
authors.affiliation: Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA.
authors.forename: Yeran
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA.
authors.forename: Changfu
authors.initials: C
authors.lastname: Yao
authors.affiliation: Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA.
authors.forename: Lu
authors.initials: L
authors.lastname: Shen
authors.affiliation: Department of Agronomy, Iowa State University, Ames, IA 50011, USA.
authors.forename: Sanzhen
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Bao
authors.affiliation: Department of Agronomy, Iowa State University, Ames, IA 50011, USA Data2Bio LLC, Ames, IA 50011, USA.
authors.forename: Patrick S
authors.initials: PS
authors.lastname: Schnable
authors.affiliation: Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA.
authors.forename: Jack
authors.initials: J
authors.lastname: Girton
authors.affiliation: Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA.
authors.forename: Jørgen
authors.initials: J
authors.lastname: Johansen
authors.affiliation: Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA kristen@iastate.edu.
authors.forename: Kristen M
authors.initials: KM
authors.lastname: Johansen
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism; D044127:Epigenesis, Genetic; D005260:Female; D049750:Genome, Insect; D006657:Histones / Q000378:metabolism*; D007814:Larva / Q000235:genetics / Q000378:metabolism; D008297:Male; D008745:Methylation; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D021381:Protein Transport; D012469:Salivary Glands / Q000378:metabolism; D059467:Transcriptome
chemical_list: D029721:Drosophila Proteins; D006657:Histones; C507306:JIL-1 protein, Drosophila; D017346:Protein Serine-Threonine Kinases