title: Evaluation of a starch-based double layer scaffold for bone regeneration in a rat model.
abstract: Damages in the maxillofacial bones are frequent in humans following trauma, metabolic diseases, neoplasia, or inflammatory processes. Many of the available treatments to regenerate bone are often ineffective. The goal of this work was to assess the in vivo behavior of an innovative double-layered scaffold based on a blend of starch and polycaprolactone (SPCL) that comprises a membrane obtained by solvent casting, which aims to act as a guided tissue regeneration membrane, and a wet-spun fiber mesh (in some cases functionalized with osteoconductive silanol groups) targeting bone regeneration. The behavior of the double layer scaffold, functionalized with silanol groups (SPCL-Si) or without (SPCL), was assessed in a mandibular rodent model and compared to a commercial collagen membrane (positive control) and to empty defects (negative control). After 8 weeks of implantation, the micro-computed tomography and the histomorphometric analysis revealed that the SPCL-Si scaffolds induced significantly higher new bone formation compared to the collagen membrane and to the empty defects, although they had a similar performance when compared to the SPCL scaffolds.
authors.affiliation: Department of Veterinary Sciences, University of Trás-os-Montes e Alto Douro, P.O. Box 1013, 5001-801, Vila Real, Portugal; 3B's Research Group-Biomaterials, Biodegradables and Biomimetics, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, University of Minho, AvePark, 4806-909, Caldas das Taipas, Guimarães, Portugal; ICVS/3B's-PT Government Associated Laboratory, Braga/Guimarães, Portugal.
authors.forename: Joao F
authors.initials: JF
authors.lastname: Requicha
authors.forename: Tiago
authors.initials: T
authors.lastname: Moura
authors.forename: Isabel B
authors.initials: IB
authors.lastname: Leonor
authors.forename: Teresa
authors.initials: T
authors.lastname: Martins
authors.forename: Fernando
authors.initials: F
authors.lastname: Muñoz
authors.forename: Rui L
authors.initials: RL
authors.lastname: Reis
authors.forename: Manuela E
authors.initials: ME
authors.lastname: Gomes
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Viegas
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D002454:Cell Differentiation; D003094:Collagen / Q000737:chemistry; D048091:Guided Tissue Regeneration; D007249:Inflammation; D008297:Male; D008334:Mandible / Q000033:anatomy & histology* / Q000503:physiopathology; D010012:Osteogenesis / Q000187:drug effects; D011091:Polyesters / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012821:Silanes / Q000737:chemistry; D013213:Starch / Q000737:chemistry*; D013997:Time Factors; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D055114:X-Ray Microtomography
chemical_list: D011091:Polyesters; D012821:Silanes; C492325:starch polycaprolactone; C082343:silanol; C016240:polycaprolactone; D013213:Starch; D003094:Collagen
keywords: bone regeneration; mandibular model; rat; scaffold; silanol groups