title: Haloactinopolyspora alkaliphila sp. nov., and emended description of the genus Haloactinopolyspora.
abstract: A facultatively alkaliphilic actinomycete strain, designated EGI 80088(T), was isolated from a saline-alkali soil sample from Xinjiang province, north-west China, and subjected to a polyphasic taxonomic characterization. Strain EGI 80088(T) formed fragmented aerial hyphae and short spore chains, and rod-like spores aggregated at maturity. Whole-cell hydrolysates of the isolate contained ll-diaminopimelic acid as the diagnostic diamino acid, and glucosamine, mannose, galactose, glucose and rhamnose as the marker sugars. The major fatty acids identified (>5%) were anteiso-C(15 : 0), iso-C(15 : 0), summed feature 4 (iso-C(17 : 1)I/anteiso-C(17 : 1)B), iso-C(16 : 0) and anteiso-C(17 : 0). The predominant menaquinone was MK-9(H4). The G+C content of the genomic DNA of strain EGI 80088(T) was 70.6 mol%. EGI 80088(T) showed the highest 16S rRNA gene sequence similarity to its closest phylogenetic neighbour Haloactinopolyspora alba YIM 93246(T) (98.5%). The DNA-DNA relatedness value of the strain EGI 80088(T) and H. alba YIM 93246(T) was 59.3±5.2%. On the basis of morphological, chemotaxonomic and phylogenetic characteristics and DNA-DNA hybridization data, strain EGI 80088(T) represents a novel species of the genus Haloactinopolyspora, for which the name Haloactinopolyspora alkaliphila sp. nov. (type strain EGI 80088(T) = BCRC 16946(T) = JCM 19128(T)) is proposed. The description of the genus Haloactinopolyspora has also been emended.
authors.affiliation: Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi 830011, PR China.
authors.forename: Yong-Guang
authors.initials: YG
authors.lastname: Zhang
authors.affiliation: The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi 830011, PR China.
authors.forename: Hong-Fei
authors.initials: HF
authors.lastname: Wang
authors.affiliation: The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, PR China.
authors.forename: Dao-Feng
authors.initials: DF
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi 830011, PR China.
authors.forename: Yuan-Ming
authors.initials: YM
authors.lastname: Zhang
authors.affiliation: Microbial Resource Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors.forename: Dong-Jin
authors.initials: DJ
authors.lastname: Park
authors.affiliation: Microbial Resource Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Kim
authors.affiliation: The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, PR China.|Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi 830011, PR China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9; D003960:Diaminopimelic Acid