title: Plastid transformation in cabbage (Brassica oleracea L. var. capitata L.) by the biolistic process.
abstract: Cabbage (Brassica oleracea L. var. capitata L.) is one of the most important vegetable crops grown worldwide. Scientists are using biotechnology in addition to traditional breeding methods to develop new cabbage varieties with desirable traits. Recent biotechnological advances in chloroplast transformation technology have opened new avenues for crop improvement. In 2007, we developed a stable plastid transformation system for cabbage and reported the successful transformation of the cry1Ab gene into the cabbage chloroplast genome. This chapter describes the methods for cabbage transformation using biolistic procedures. The following sections are included in this protocol: preparation of donor materials, coating gold particles with DNA, biolistic bombardment, as well as the regeneration and selection of transplastomic cabbage plants. The establishment of a plastid transformation system for cabbage offers new possibilities for introducing new agronomic and horticultural traits into Brassica crops.
authors.affiliation: Department of Horticulture, National Chung Hsing University, Taichung, Taiwan, ROC.
authors.forename: Menq-Jiau
authors.initials: MJ
authors.lastname: Tseng
authors.forename: Ming-Te
authors.initials: MT
authors.lastname: Yang
authors.forename: Wan-Ru
authors.initials: WR
authors.lastname: Chu
authors.forename: Cheng-Wei
authors.initials: CW
authors.lastname: Liu
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D019470:Biolistics / Q000379:methods*; D001937:Brassica / Q000235:genetics*; D002736:Chloroplasts / Q000235:genetics*; D018556:Crops, Agricultural; D004351:Drug Resistance / Q000235:genetics; D009713:Nucleotidyltransferases / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D000198:Spectinomycin / Q000494:pharmacology; D013307:Streptomycin / Q000494:pharmacology; D014170:Transformation, Genetic
chemical_list: D000900:Anti-Bacterial Agents; D000198:Spectinomycin; D000594:Amino Acid Oxidoreductases; D009713:Nucleotidyltransferases; C020565:gentamicin 2''-nucleotidyltransferase; D013307:Streptomycin