title: Loss of CDC14B expression in clear cell renal cell carcinoma: meta-analysis of microarray data sets.
abstract: OBJECTIVE
  To discover significant differentially expressed genes (DEGs) in clear cell renal cell carcinoma (ccRCC) that might be unidentified by single microarray analysis.
  
  
  METHODS
  The effect sizes of five ccRCC microarray data sets were combined using a random-effects model. The most downregulated gene was validated in paired 80 ccRCC tissues by immunohistochemistry.
  
  
  RESULTS
  CDC14B was the most downregulated gene among 1,761 DEGs. CDC14B was strongly expressed in the apical proximal tubules in the nonneoplastic tissues, while it was completely absent in 10 (12.5%) of 80 or downregulated in 70 (87.5%) of 80 ccRCC cases. The complete loss of CDC14B correlated with high T stage (P = .038), advanced TNM stage (P = .027), tumor recurrence (P = .038), and shorter recurrence-free survival (P = .046) compared with the partial loss of CDC14B.
  
  
  CONCLUSIONS
  Microarray meta-analysis is a useful tool for pathologists. CDC14B expression is downregulated in ccRCC, suggesting its role in renal carcinogenesis.
authors.affiliation: Dept of Pathology, Korea University Ansan Hospital, 516, Gojan-1 Dong, Danwon-Gu, Ansan-Si, Gyeonggi-Do, 425-707, Republic of Korea; e-mail: apysk@korea.ac.kr.
authors.forename: Younghye
authors.initials: Y
authors.lastname: Kim
authors.forename: Jung-Woo
authors.initials: JW
authors.lastname: Choi
authors.forename: Ju-Han
authors.initials: JH
authors.lastname: Lee
authors.forename: Young-Sik
authors.initials: YS
authors.lastname: Kim
mesh_terms: D000328:Adult; D000368:Aged; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000401:mortality; D015536:Down-Regulation; D054637:Dual-Specificity Phosphatases / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism; D007680:Kidney Neoplasms / Q000235:genetics* / Q000401:mortality; D008297:Male; D046228:Microarray Analysis; D008875:Middle Aged
chemical_list: C474200:CDC14B protein, human; D054637:Dual-Specificity Phosphatases
keywords: CDC14B; Meta-analysis; Microarray; Renal cell carcinoma