title: CD41 is a reliable identification and activation marker for murine basophils in the steady state and during helminth and malarial infections.
abstract: Basophils, a rare leukocyte population in peripheral circulation, are conventionally identified as CD45(int) CD49b(+) FcεRI(+) cells. Here, we show that basophils from blood and several organs of naïve wild-type mice express CD41, the α subunit of α(IIb)β₃ integrin. CD41 expression on basophils is upregulated after in vivo IL-3 treatment and during infection with Nippostrongylus brasiliensis (Nb). Moreover, CD41 can be used as a reliable marker for basophils, circumventing technical difficulties associated with FcεRI for basophil identification in a Nb infection model. In vitro anti-IgE cross-linking and IL-3 basophil stimulation showed that CD41 upregulation positively correlates with augmented surface expression of CD200R and increased production of IL-4/IL-13, indicating that CD41 is a basophil activation marker. Furthermore, we found that infection with Plasmodium yoelii 17X (Py17x) induced a profound basophilia and using Mcpt8(DTR) reporter mice as a basophil-specific depletion model, we verified that CD41 can be used as a marker to track basophils in the steady state and during infection. During malarial infection, CD41 expression on basophils is negatively regulated by IFN-γ and positively correlates with increased basophil IL-4 production. In conclusion, we provide evidence that CD41 can be used as both an identification and activation marker for basophils during homeostasis and immune challenge.
authors.affiliation: Singapore Immunology Network (SIgN), Agency for Science Technology and Research (A*STAR), Biopolis, Singapore.
authors.forename: Nadja
authors.initials: N
authors.lastname: Bakocevic
authors.forename: Carla
authors.initials: C
authors.lastname: Claser
authors.forename: Soichiro
authors.initials: S
authors.lastname: Yoshikawa
authors.forename: Leigh Ann
authors.initials: LA
authors.lastname: Jones
authors.forename: Samantha
authors.initials: S
authors.lastname: Chew
authors.forename: Chi Ching
authors.initials: CC
authors.lastname: Goh
authors.forename: Benoit
authors.initials: B
authors.lastname: Malleret
authors.forename: Anis
authors.initials: A
authors.lastname: Larbi
authors.forename: Florent
authors.initials: F
authors.lastname: Ginhoux
authors.forename: Maria Curotto
authors.initials: MC
authors.lastname: de Lafaille
authors.forename: Hajime
authors.initials: H
authors.lastname: Karasuyama
authors.forename: Laurent
authors.initials: L
authors.lastname: Renia
authors.forename: Lai Guan
authors.initials: LG
authors.lastname: Ng
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D001491:Basophils / Q000276:immunology* / Q000473:pathology; D005260:Female; D007073:Immunoglobulin E / Q000276:immunology; D007377:Interleukin-3 / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D008288:Malaria / Q000276:immunology* / Q000473:pathology; D008562:Membrane Glycoproteins / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D009559:Nippostrongylus / Q000276:immunology*; D010967:Plasmodium yoelii / Q000276:immunology*; D040201:Platelet Membrane Glycoprotein IIb / Q000276:immunology*; D017206:Strongylida Infections / Q000276:immunology* / Q000473:pathology
chemical_list: D000909:Antibodies, Helminth; C492346:CD200 receptor, mouse; D007377:Interleukin-3; D008562:Membrane Glycoproteins; D040201:Platelet Membrane Glycoprotein IIb; D015847:Interleukin-4; D007073:Immunoglobulin E
keywords: Basophils; CD41; Helminths; Malaria