title: Production of antigen-loaded biodegradable nanoparticles and uptake by dendritic cells.
abstract: Particle-based cancer vaccines prepared from biodegradable polymers are a potentially attractive way of delivering antigen alone, or in combination with adjuvant molecules, to dendritic cells (DC). Non-viral modes of vaccination must ensure the activation of cellular immune responses in addition to humoral responses if an effective antitumor immune response is to be initiated. Here we describe in detail a method for manufacturing poly(D,L-lactide-co-glycolide) (PLGA) particles loaded with purified protein/antigen. We also describe a method for generating bone marrow-derived DC in vitro. These DC can be subsequently used to measure the adjuvant properties of the particle formulations.
authors.affiliation: College of Pharmacy, University of Iowa, Iowa City, IA, USA.
authors.forename: Vijaya Bharti
authors.initials: VB
authors.lastname: Joshi
authors.forename: Sean M
authors.initials: SM
authors.lastname: Geary
authors.forename: Aliasger K
authors.initials: AK
authors.lastname: Salem
mesh_terms: D000818:Animals; D000941:Antigens / Q000737:chemistry* / Q000276:immunology; D001692:Biological Transport; D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D007700:Kinetics; D019344:Lactic Acid / Q000737:chemistry* / Q000378:metabolism*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism*; D009369:Neoplasms / Q000276:immunology; D011100:Polyglycolic Acid / Q000737:chemistry* / Q000378:metabolism*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer
chemical_list: D000941:Antigens; D004337:Drug Carriers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid