title: Genetic predisposition to essential hypertension in children: analysis of 17 single nucleotide polymorphisms.
abstract: Study of 17 single nucleotide polymorphisms has been performed to determine the factors of genetic predisposition to essential hypertension. Polymerase chain reaction (PCR) with subsequent analysis of restriction fragment length, allele specific PCR or real-time PCR was used for genotyping of 17 single nucleotide polymorphisms in 14 genes in 145 children with essential hypertension and 144 healthy persons with following complex multivariate statistical analysis. Two single nucleotide polymorphisms--MMP9 (C(-1562) --> T) and NOS3 (Glu298 --> Asp)--rs3918242 and rs1799983--were shown to represent the main independent effects with the highest predictive potential (77.1% as indicated by binary logistic regression and 74.6% testing accuracy shown by Multifactorial Dimensionality Reduction). MMP9 (C(-1562 --> T) and NOS3 (Glu298 --> Asp) potentially may be used to create predictive algorithm for determination of predisposition to arterial hypertension in children.
authors.affiliation: O.O. Bogomoletz Institute of Physiology, NAS of Ukraine, Kiev. sergei_goncharov@bigmir.net
authors.forename: S V
authors.initials: SV
authors.lastname: Goncharov
authors.affiliation: O.O. Bogomoletz Institute of Physiology, NAS of Ukraine, Kiev.
authors.forename: V L
authors.initials: VL
authors.lastname: Gurianova
authors.affiliation: O.O. Bogomoletz Institute of Physiology, NAS of Ukraine, Kiev.
authors.forename: D O
authors.initials: DO
authors.lastname: Stroy
authors.affiliation: O.O. Bogomoletz Institute of Physiology, NAS of Ukraine, Kiev.
authors.forename: T I
authors.initials: TI
authors.lastname: Drevytska
authors.affiliation: O.O. Bogomoletz Institute of Physiology, NAS of Ukraine, Kiev.
authors.forename: S P
authors.initials: SP
authors.lastname: Kaplinskii
authors.affiliation: O.O. Bogomoletz Institute of Physiology, NAS of Ukraine, Kiev.
authors.forename: E A
authors.initials: EA
authors.lastname: Nastenko
authors.affiliation: O.O. Bogomoletz Institute of Physiology, NAS of Ukraine, Kiev.
authors.forename: M
authors.initials: M
authors.lastname: Litvinenko
authors.affiliation: O.O. Bogomoletz Institute of Physiology, NAS of Ukraine, Kiev.
authors.forename: R V
authors.initials: RV
authors.lastname: Terletskiy
authors.affiliation: O.O. Bogomoletz Institute of Physiology, NAS of Ukraine, Kiev.
authors.forename: M V
authors.initials: MV
authors.lastname: Khaitovych
authors.affiliation: O.O. Bogomoletz Institute of Physiology, NAS of Ukraine, Kiev.
authors.forename: O O
authors.initials: OO
authors.lastname: Moibenko
authors.affiliation: O.O. Bogomoletz Institute of Physiology, NAS of Ukraine, Kiev.
authors.forename: V E
authors.initials: VE
authors.lastname: Dosenko
mesh_terms: D000293:Adolescent; D000465:Algorithms; D016022:Case-Control Studies; D002648:Child; D003627:Data Interpretation, Statistical; D000075222:Essential Hypertension; D020022:Genetic Predisposition to Disease; D006439:Hemodynamics / Q000235:genetics*; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D016015:Logistic Models; D015999:Multivariate Analysis; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D011237:Predictive Value of Tests