title: The gene cluster instability (GCI) assay for recombination.
abstract: A newly developed method for quantitatively detecting genomic restructuring in cultured human cell lines as the result of recombination is presented: the "gene cluster instability" (GCI) assay. The assay is physiological in that it detects spontaneous restructuring without the need for exogenous recombination-initiating treatments such as DNA damage. As an assay for genotoxicity, the GCI assay is complementary to well-established sister chromatid exchange (SCE) methods. Analysis of the U-2 OS osteosarcoma cell line is presented as an illustration of the method.
authors.affiliation: Markey Cancer Center, University of Kentucky, Lexington, KY, USA.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Killen
authors.forename: Dawn M
authors.initials: DM
authors.lastname: Stults
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Pierce
mesh_terms: D015139:Blotting, Southern / Q000592:standards; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D015342:DNA Probes / Q000737:chemistry; D004275:DNA, Ribosomal / Q000235:genetics; D004587:Electrophoresis, Agar Gel / Q000592:standards; D042822:Genomic Instability; D059765:Homologous Recombination; D006801:Humans; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D012015:Reference Standards
chemical_list: D015342:DNA Probes; D004275:DNA, Ribosomal