title: Differential induction of apoptosis, interferon signaling, and phagocytosis in macrophages infected with a panel of attenuated and nonattenuated poxviruses.
abstract: Due to the essential role macrophages play in antiviral immunity, it is important to understand the intracellular and molecular processes that occur in macrophages following infection with various strains of vaccinia virus, particularly those used as vaccine vectors. Similarities as well as differences were found in macrophages infected with different poxvirus strains, particularly at the level of virus-induced apoptosis and the expression of immunomodulatory genes, as determined by microarray analyses. Interestingly, the attenuated modified vaccinia Ankara virus (MVA) was particularly efficient in triggering apoptosis and beta interferon (IFN-β) secretion and in inducing changes in the expression of genes associated with increased activation of innate immunity, setting it apart from the other five vaccinia virus strains tested. Taken together, these results increase our understanding of how these viruses interact with human macrophages, at the cellular and molecular levels, and suggest mechanisms that may underlie their utility as recombinant vaccine vectors.
  
  
  OBJECTIVE
  Our studies clearly demonstrate that there are substantial biological differences in the patterns of cellular gene expression between macrophages infected with different poxvirus strains and that these changes are due specifically to infection with the distinct viruses. For example, a clear induction in IFN-β mRNA was observed after infection with MVA but not with other poxviruses. Importantly, antiviral bioassays confirmed that MVA-infected macrophages secreted a high level of biologically active type I IFN. Similarly, the phagocytic capacity of macrophages was also specifically increased after infection with MVA. Although the main scope of this study was not to test the vaccine potential of MVA as there are several groups in the field working extensively on this aspect, the characteristics/phenotypes we observed at the in vitro level clearly highlight the inherent advantages that MVA possesses in comparison to other poxvirus strains.
authors.affiliation: Department of Preventive Medicine, Public Health and Microbiology, Universidad Autónoma, Madrid, Spain.
authors.forename: Sandra
authors.initials: S
authors.lastname: Royo
authors.forename: Bruno
authors.initials: B
authors.lastname: Sainz
authors.forename: Enrique
authors.initials: E
authors.lastname: Hernández-Jiménez
authors.forename: Hugh
authors.initials: H
authors.lastname: Reyburn
authors.forename: Eduardo
authors.initials: E
authors.lastname: López-Collazo
authors.forename: Susana
authors.initials: S
authors.lastname: Guerra
mesh_terms: D017209:Apoptosis; D002478:Cells, Cultured; D020869:Gene Expression Profiling; D006801:Humans; D007372:Interferons / Q000276:immunology*; D008264:Macrophages / Q000276:immunology* / Q000821:virology*; D046228:Microarray Analysis; D010587:Phagocytosis; D011212:Poxviridae / Q000276:immunology*; D015398:Signal Transduction
chemical_list: D007372:Interferons