title: Long-term intake of pea fiber affects colonic barrier function, bacterial and transcriptional profile in pig model.
abstract: The objective of this study was to investigate the effect of long-term intake of pea fiber (PF) on colonic health and the underlying mechanism. Fifty weaned piglets were randomly allocated into 2 groups receiving control and fibrous diet with inclusion of PF from weaning to Day 160 postweaning, with 5 pens in each group and 5 pigs in each pen. Compared with control diet, long-term intake of PF diet induced deeper crypt and increased colonic barrier-related protein expression of mucin and sIgA. As indicated by the increased lactobacillus content, pigs fed PF diet had a stimulating effect on bacterial fermentation in the colon, thus a higher concentration of colonic total short chain fatty acid and acetic acid were observed. DNA microarray results suggested that feeding PF diet inhibited 77% of genes (40 downregulated and 12 upregulated genes) related to colonic cancer, immune response, and lipid metabolism, involving in signal pathway such as intestinal immune network for IgA production, peroxisome proliferator-activated receptor signaling pathway and nutrient metabolism-related pathways. Collectively, our results suggested that long-term intake of PF would improve colonic function via altering colonic barriers, colonic immune and metabolism-related protein or gene expressions.
authors.affiliation: a Institute of Animal Nutrition, Sichuan Agricultural University, Sichuan, People's Republic of China and Key Laboratory of Animal Disease-Resistance Nutrition, Sichuan Agricultural University , Sichuan , People's Republic of China.
authors.forename: Lianqiang
authors.initials: L
authors.lastname: Che
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Bing
authors.initials: B
authors.lastname: Yu
authors.forename: Jun
authors.initials: J
authors.lastname: He
authors.forename: Ping
authors.initials: P
authors.lastname: Zheng
authors.forename: Xiangbing
authors.initials: X
authors.lastname: Mao
authors.forename: Jie
authors.initials: J
authors.lastname: Yu
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Huang
authors.forename: Daiwen
authors.initials: D
authors.lastname: Chen
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D003106:Colon / Q000187:drug effects* / Q000382:microbiology / Q000502:physiology*; D004043:Dietary Fiber / Q000494:pharmacology*; D005232:Fatty Acids, Volatile / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D007778:Lactobacillus / Q000187:drug effects; D009077:Mucins / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D018532:Pisum sativum; D010455:Peptides / Q000378:metabolism; D013552:Swine / Q000254:growth & development; D000071164:Trefoil Factor-2
chemical_list: D001426:Bacterial Proteins; D004043:Dietary Fiber; D005232:Fatty Acids, Volatile; D009077:Mucins; D010455:Peptides; D000071164:Trefoil Factor-2; C070683:surface layer protein A, Bacteria