title: The sister chromatid exchange (SCE) assay.
abstract: A fully optimized staining method for detecting sister chromatid exchanges in cultured cells is presented. The method gives reproducibly robust quantitative results. Sister chromatid exchange is a classic toxicology assay for genotoxicity and for detecting alterations to the biochemistry underlying cellular homologous recombination. Growth of cells in the presence of 5'-bromo-deoxyuridine for two rounds of DNA replication followed by collecting metaphase spreads on glass slides, treatment with the UV-sensitive dye Hoechst 33258, long-wave UV light exposure, and Giemsa staining gives a permanent record of the exchanges.
authors.affiliation: Markey Cancer Center, University of Kentucky, 222 Combs Research Building, 800 Rose Street, Lexington, KY, 40536-0096, USA.
authors.forename: Dawn M
authors.initials: DM
authors.lastname: Stults
authors.forename: Michael W
authors.initials: MW
authors.lastname: Killen
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Pierce
mesh_terms: D001973:Bromodeoxyuridine / Q000378:metabolism; D004261:DNA Replication; D006367:HeLa Cells; D006801:Humans; D020014:K562 Cells; D008677:Metaphase; D059767:Recombinational DNA Repair; D012854:Sister Chromatid Exchange; D013194:Staining and Labeling
chemical_list: D001973:Bromodeoxyuridine