title: Direct and single-molecule visualization of the solution-state structures of G-hairpin and G-triplex intermediates.
abstract: We present the direct and single-molecule visualization of the in-pathway intermediates of the G-quadruplex folding that have been inaccessible by any experimental method employed to date. Using DNA origami as a novel tool for the structural control and high-speed atomic force microscopy (HS-AFM) for direct visualization, we captured images of the unprecedented solution-state structures of a tetramolecular antiparallel and (3+1)-type G-quadruplex intermediates, such as G-hairpin and G-triplex, with nanometer precision. No such structural information was reported previously with any direct or indirect technique, solution or solid-state, single-molecule or bulk studies, and at any resolution. Based on our results, we proposed a folding mechanism of these G-quadruplexes.
authors.affiliation: Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502 (Japan); Current address: Faculty of Medicine and Life Science Center of TARA, University of Tsukuba, 1-1-1 Tennodai, Tsukuba-shi, Ibaraki-ken 305-8577 (Japan).
authors.forename: Arivazhagan
authors.initials: A
authors.lastname: Rajendran
authors.forename: Masayuki
authors.initials: M
authors.lastname: Endo
authors.forename: Kumi
authors.initials: K
authors.lastname: Hidaka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sugiyama
mesh_terms: D004247:DNA / Q000737:chemistry*; D054856:G-Quadruplexes; D018625:Microscopy, Atomic Force / Q000379:methods*; D009690:Nucleic Acid Conformation
chemical_list: D004247:DNA
keywords: DNA origami; G-hairpin; G-quadruplex; G-triplex; atomic force microscopy