title: In vitro and in vivo pathogenicity studies of Pasteurella multocida strains harbouring different ompA.
abstract: Pasteurella multocida is a pathogenic, Gram-negative bacterium that is commonly found as normal flora in nasopharynx of variety of wild and domestic animals. Numerous virulence factors have been described for P. multocida isolates which include adherence and colonization factors, iron-regulated and acquisition proteins, extracellular enzymes such as neuraminidase, lipopolysaccharide (LPS), capsule and a variety of outer membrane proteins (Omp). OmpA has a significant role in stabilizing the cell envelope structure by providing physical linkage between the outer membrane & peptidoglycan. It has been shown to mediate P. multocida -host cells interaction via heparin and/or fibronectin binding and therefore act as an important invasive molecule which could determine the final outcome of initial infection. Comparative nucleotide sequence analysis of ompA gene of P. multocida has revealed that despite extensive genetic diversity in ompA of P. multocida, most sequences could be classified into two major allele classes namely ompA allele (I) and allele (II). The P. multocida recovered from nasal cavity of bovine and belonging to two ompA classes were tested for their differential virulence. In vitro pathogenicity studies on Madin Darby Bovine Kidney (MDBK) cell line employing adhesion and invasion assays indicated that P. multocida strain with ompA (I) is more invasive than P. multocida strain with ompA (II). In vivo studies in mice further reiterated that the isolates harbouring ompA(I) were comparatively more virulent to isolates harbouring ompA (II).
authors.affiliation: Department of Veterinary Microbiology, Dr. G.C. Negi College of Veterinary & Animal Sciences, CSKHPKV, Palampur, Himachal Pradesh, 176062, India.
authors.forename: Shailja
authors.initials: S
authors.lastname: Katoch
authors.forename: Mandeep
authors.initials: M
authors.lastname: Sharma
authors.forename: R D
authors.initials: RD
authors.lastname: Patil
authors.forename: Sandeep
authors.initials: S
authors.lastname: Kumar
authors.forename: Subhash
authors.initials: S
authors.lastname: Verma
mesh_terms: D000483:Alleles; D000825:Animal Structures / Q000382:microbiology; D000818:Animals; D058491:Bacterial Load; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D051379:Mice; D008969:Molecular Sequence Data; D010326:Pasteurella Infections / Q000382:microbiology* / Q000401:mortality / Q000473:pathology; D016979:Pasteurella multocida / Q000235:genetics* / Q000472:pathogenicity*; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D037521:Virulence Factors; C078167:OMPA outer membrane proteins