title: In vitro study of electroactive tetraaniline-containing thermosensitive hydrogels for cardiac tissue engineering.
abstract: Injectable hydrogels made of degradable biomaterials can function as both physical support and cell scaffold in preventing infarct expansion and promoting cardiac repair in myocardial infarction therapy. Here, we report in situ hydrogels consisting of thermosensitive PolyNIPAM-based copolymers and electroactive tetraaniline (TA). Studies showed that the addition of 2-methylene-1,3-dioxepane (MDO) provided the PolyNIPAM-based gel with biodegradability, and the introduction of tetraaniline endowed these copolymers with desirable electrical properties and antioxidant activities. The encapsulated H9c2 cells (rat cardiac myoblast) remained highly viable in the gel matrices. In vivo gel formation and histological analyses were performed in rats by subcutaneous injection and excellent biocompatibility was observed. Furthermore, the proliferation and intracellular calcium transients of H9c2 cells were also studied with (and without) electrical stimuli. Both in vitro and in vivo results demonstrated that electroactive hydrogel may be used as a promising injectable biomaterial for cardiac tissue engineering.
authors.affiliation: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun 130022, People's Republic of China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Cui
authors.forename: Yadong
authors.initials: Y
authors.lastname: Liu
authors.forename: Yilong
authors.initials: Y
authors.lastname: Cheng
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhang
authors.forename: Peibiao
authors.initials: P
authors.lastname: Zhang
authors.forename: Xuesi
authors.initials: X
authors.lastname: Chen
authors.forename: Yen
authors.initials: Y
authors.lastname: Wei
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D000814:Aniline Compounds / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D004558:Electric Stimulation; D020100:Hydrogels / Q000737:chemistry*; D008422:Materials Testing; D032386:Myoblasts, Cardiac / Q000166:cytology*; D011108:Polymers / Q000737:chemistry; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species; D012996:Solutions; D013696:Temperature; D023822:Tissue Engineering
chemical_list: D000178:Acrylamides; D000814:Aniline Compounds; D001672:Biocompatible Materials; D020100:Hydrogels; D011108:Polymers; D017382:Reactive Oxygen Species; D012996:Solutions; C067295:N-isopropylacrylamide; C023650:aniline; D002118:Calcium