title: Efficacy of some antioxidants supplementation in reducing oxidative stress post sodium tungstate exposure in male wistar rats.
abstract: This study aimed to evaluate the protective efficacy of some antioxidants against sodium tungstate induced oxidative stress in male wistar rats. Animals were sub-chronically exposed to sodium tungstate (100ppm in drinking water) for three months except for control group. In the same time, many rats were supplemented orally with different antioxidants (alpha-lipoic acid (ALA), n-acetylcysteine (NAC), quercetin or naringenin (0.30mM)) for five consecutive days a week for the same mentioned period before. Exposure to sodium tungstate significantly (P<0.05) inhibit blood δ-aminolevulinic acid dehydratase (ALAD) activity, liver and blood reduced glutathione (GSH) levels and an increase in oxidized glutathione (GSSG) and thiobarbituric acid reactive species (TBARS) levels in tissues. ALA acid and NAC supplementation post sodium tungstate exposure increased GSH and also, was beneficial in the recovery of altered superoxide dismutase and catalase activity, besides, significantly reducing blood and tissue reactive oxygen species and TBARS levels. The results suggest a more pronounced efficacy of ALA acid and NAC supplementation than quercetin or naringenin supplementation post sodium tungstate exposure in preventing induced oxidative stress in rats.
authors.affiliation: Division of Regulatory Toxicology, Defence Research and Development Establishment, Jhansi Road, Gwalior 474 002, India.
authors.forename: S
authors.initials: S
authors.lastname: Sachdeva
authors.affiliation: Division of Regulatory Toxicology, Defence Research and Development Establishment, Jhansi Road, Gwalior 474 002, India. Electronic address: sjsflora@drde.drdo.in.
authors.forename: S J S
authors.initials: SJS
authors.lastname: Flora
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D019587:Dietary Supplements; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D000623:Porphobilinogen Synthase / Q000097:blood; D017208:Rats, Wistar; D013154:Spleen / Q000187:drug effects / Q000473:pathology; D017973:Tungsten Compounds / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D017973:Tungsten Compounds; C025399:sodium tungstate(VI); D000623:Porphobilinogen Synthase
keywords: Antioxidant; Flavonoids; Oxidative stress; Rats; Sodium tungstate toxicity