title: Role of Nogo‑A in the regulation of hepatocellular carcinoma SMMC‑7721 cell apoptosis.
abstract: Nogo-A has been identified as an inhibitor of neurite outgrowth specific to the central nervous system. However, little is known about the role of Nogo-A in hepatocellular carcinoma (HCC), the most common primary malignant tumor with a high mortality rate. This study aimed to investigate the role of endogenous Nogo-A in human liver cancer cells. Reverse transcription polymerase chain reaction was used to detect the expression of Nogo-A in four liver cancer cell lines. A lentivirus vector was then constructed to mediate RNA interference (RNAi) targeting of Nogo‑A (LV‑Nogo-A‑siRNA) and was confirmed to successfully suppress the expression of the Nogo-A gene in SMMC-7721 cells. Furthermore, Nogo-A was observed to be highly expressed in liver cancer cell lines. RNAi of Nogo-A using the LV‑Nogo-A‑siRNA construct significantly decreased Nogo-A protein expression and specifically inhibited the growth of SMMC-7721 cells. This growth inhibitory effect may be attributed to an increase in G2/M phase arrest and apoptosis in SMMC-7721 cells containing Nogo-A‑siRNA. The results of this study demonstrate that Nogo-A may represent a novel therapeutic target for the treatment of liver cancer, in addition to its potent roles in neural systems.
authors.affiliation: Bio‑X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiaotong University, Shanghai 200030, P.R. China.
authors.forename: Chun-Qiu
authors.initials: CQ
authors.lastname: Hao
authors.affiliation: Centre of Liver Diseases, Fourth Military Medical University, Tangdu Hospital, Xi'an, Shanxi 710038, P.R. China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Centre of Liver Diseases, Fourth Military Medical University, Tangdu Hospital, Xi'an, Shanxi 710038, P.R. China.
authors.forename: Jiu-Ping
authors.initials: JP
authors.lastname: Wang
authors.affiliation: Centre of Liver Diseases, Fourth Military Medical University, Tangdu Hospital, Xi'an, Shanxi 710038, P.R. China.
authors.forename: Mei-Jun
authors.initials: MJ
authors.lastname: Peng
authors.affiliation: Centre of Liver Diseases, Fourth Military Medical University, Tangdu Hospital, Xi'an, Shanxi 710038, P.R. China.
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Xie
authors.affiliation: Centre of Liver Diseases, Fourth Military Medical University, Tangdu Hospital, Xi'an, Shanxi 710038, P.R. China.
authors.forename: Wen-Zhen
authors.initials: WZ
authors.lastname: Kang
authors.affiliation: Centre of Liver Diseases, Fourth Military Medical University, Tangdu Hospital, Xi'an, Shanxi 710038, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.affiliation: Centre of Liver Diseases, Fourth Military Medical University, Tangdu Hospital, Xi'an, Shanxi 710038, P.R. China.
authors.forename: Ping-Zhong
authors.initials: PZ
authors.lastname: Wang
authors.affiliation: Bio‑X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiaotong University, Shanghai 200030, P.R. China.
authors.forename: Chun-Ling
authors.initials: CL
authors.lastname: Wan
authors.affiliation: Bio‑X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiaotong University, Shanghai 200030, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: He
authors.affiliation: Bio‑X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiaotong University, Shanghai 200030, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Cai
authors.affiliation: Centre of Liver Diseases, Fourth Military Medical University, Tangdu Hospital, Xi'an, Shanxi 710038, P.R. China.
authors.forename: Zhang-Sheng
authors.initials: ZS
authors.lastname: Jia
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D059447:Cell Cycle Checkpoints / Q000235:genetics; D045744:Cell Line, Tumor; D017353:Gene Deletion; D015870:Gene Expression; D023061:Gene Order; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D008113:Liver Neoplasms / Q000235:genetics*; D009185:Myelin Proteins / Q000235:genetics*; D000070798:Nogo Proteins; D034622:RNA Interference
chemical_list: D009185:Myelin Proteins; D000070798:Nogo Proteins; C000603199:RTN4 protein, human