title: Simultaneous determination of cotinine and trans-3-hydroxycotinine in urine by automated solid-phase extraction using gas chromatography-mass spectrometry.
abstract: A gas chromatography-mass spectrometry method was developed and validated for the simultaneous automated solid-phase extraction and quantification of cotinine and trans-3-hydroxycotinine in human urine. Good linearity was observed over the concentration ranges studied (R(2) > 0.99). The limit of quantification was 10 ng/mL for both analytes. The limits of detection were 0.06 ng/mL for cotinine (COT) and 0.02 ng/mL for trans-3-hydroxycotinine (OH-COT). Accuracy for COT ranged from 0.98 to 5.28% and the precision ranged from 1.24 to 8.78%. Accuracy for OH-COT ranged from -2.66 to 3.72% and the precision ranged from 3.15 to 7.07%. Mean recoveries for cotinine and trans-3-hydroxycotinine ranged from 77.7 to 89.1%, and from 75.4 to 90.2%, respectively. This analytical method for the simultaneous measurement of cotinine and trans-3-hydroxycotinine in urine will be used to monitor tobacco smoking in pregnant women and will permit the usefulness of trans-3-hydroxycotinine as a specific biomarker of tobacco exposure to be determined.
authors.affiliation: Laboratoire de toxicologie de médecine légale, Hôpital Jean Verdier - APHP, Avenue du 14 juillet, 93140, Bondy, France.
authors.forename: Fouad
authors.initials: F
authors.lastname: Chiadmi
authors.forename: Joël
authors.initials: J
authors.lastname: Schlatter
mesh_terms: D003367:Cotinine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000652:urine*; D004355:Drug Stability; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: C001381:hydroxycotinine; D003367:Cotinine
keywords: GCMS; SPE; cotinine; trans-3-hydroxycotinine; urine