title: Assessment of TGF-β3 on production of aggrecan by human articular chondrocytes in pellet culture system.
abstract: BACKGROUND
  The Autologous Chondrocytes Transplantation (ACT) method is being studied for repair of cartilage diseases. As the chondrocytes dedifferentiated during monolayer culture, three-dimensional cultures are suggested to redifferentiate them. The aim of this study was investigation of the effect of TGF-β3 growth factor on chondrocytes in pellet culture system.
  
  
  METHODS
  The chondrocytes were isolated from three human articular cartilages by enzymatic digestion. The cells of the second passage were transferred to pellet culture system. We determined the chondrogenic medium with TGF-β3 as the experimental group and without it as the control group. After 2 weeks, the aggrecan production was investigated using histological and immunohistochemical (IHC) methods.
  
  
  RESULTS
  The presence of glycosaminoglycans was proved through Toluiden blue staining. Comparison of IHC results using MATLAB software showed that aggrecan in the experimental group was significantly higher than in the control group (P ≤ 0.05).
  
  
  CONCLUSIONS
  The presence of TGF-β3 in the chondrogenic medium could lead to the production of more aggrecan in chondrocytes cultivated in pellet culture system.
authors.affiliation: Department of Anatomical Sciences and Molecular Biology, Medical Faculty, Isfahan University of Medical Sciences, Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Zamani
authors.affiliation: Department of Anatomical Sciences and Molecular Biology, Medical Faculty, Isfahan University of Medical Sciences, Iran.
authors.forename: Batool
authors.initials: B
authors.lastname: Hashemibeni
authors.affiliation: Department of Anatomical Sciences and Molecular Biology, Medical Faculty, Isfahan University of Medical Sciences, Iran.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Esfandiari
authors.affiliation: Department of Anatomical Sciences and Molecular Biology, Medical Faculty, Isfahan University of Medical Sciences, Iran.
authors.forename: Azadeh
authors.initials: A
authors.lastname: Kabiri
authors.affiliation: Department of Biophysics, Medical Faculty, Isfahan University of Medical Sciences, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Rabbani
authors.affiliation: Department of Anatomical Sciences and Molecular Biology, Medical Faculty, Isfahan University of Medical Sciences, Iran.
authors.forename: Roshanak
authors.initials: R
authors.lastname: Abutorabi
keywords: Aggrecan; TGF-β3; chondrocyte; growth factor; pellet system