title: Simultaneous quantitation of nucleosides, nucleobases, amino acids, and alkaloids in mulberry leaf by ultra high performance liquid chromatography with triple quadrupole tandem mass spectrometry.
abstract: An ultra high performance liquid chromatography coupled with a triple quadrupole mass detection and electrospray ionization mass spectrometry method has been established for the simultaneous determination of the 14 nucleosides and nucleobases, 24 amino acids and two main alkaloids in mulberry leaf. In this method, a complicated mobile phase, the flow rate of which was 0.4 mL/min, was applied to the gradient elution, which provided a satisfied separation of the 40 compounds. The present method was validated, and sufficient reproducibility and accuracy were obtained for the quantitative measurement of the 40 compounds. The method was subsequently applied to ten mulberry leaves and the results showed that almost all of these samples were rich in nucleosides, nucleobases, amino acids, and alkaloids. The proposed method, which is convenient and economical, could serve as a prerequisite for the quality control of mulberry leaf herbs and be applied analogously to other Chinese medicines.
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing, China.
authors.forename: Li-li
authors.initials: LL
authors.lastname: Zhang
authors.forename: Yong-liang
authors.initials: YL
authors.lastname: Bai
authors.forename: Shu-lan
authors.initials: SL
authors.lastname: Shu
authors.forename: Da-wei
authors.initials: DW
authors.lastname: Qian
authors.forename: Zhen
authors.initials: Z
authors.lastname: Ou-yang
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Jin-ao
authors.initials: JA
authors.lastname: Duan
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D000596:Amino Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031625:Morus / Q000737:chemistry*; D009705:Nucleosides / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000470:Alkaloids; D000596:Amino Acids; D009705:Nucleosides; D010936:Plant Extracts
keywords: Alkaloids; Amino acids; Morus Alba; Nucleosides; Simultaneous quantitation