title: A fast and validated method for the determination of malondialdehyde in fish liver using high-performance liquid chromatography with a photodiode array detector.
abstract: Malondialdehyde (MDA) is a biomarker of lipid peroxidation and is present in foods and biological samples such as plasma. A high-performance liquid chromatography (HPLC) method was applied to determine MDA in fish liver samples after derivatization with 2,4-dinitrophenylhydrazine (DNPH) using a ODS2 column (10 cm × 4.6 mm, 3 μm) and a photodiode array detector. The mobile phase consisted of 0.2% acetic acid (v/v) in distilled water and acetonitrile (42:58, v/v). The present method was validated in terms of linearity, lower limit of quantification, lower limit of detection, precision, accuracy, recovery, and stability of MDA according to U.S. Food and Drug Administration (FDA) guidelines. The limit of quantification of MDA was 0.39 μmol/L, which is comparable to other methods. The recovery of the spiked MDA liver samples was in the range of 92.4% to 104.2%. This newly modified HPLC method is specific, sensitive, and accurate and allows the analysis of MDA within 4 min in fish liver but also in other tissues and plasma.
authors.affiliation: Inst. of Human Nutrition and Food Science, Christian-Albrechts-Univ. of Kiel, Hermann-Rodewald St. 6, 24118, Kiel, Germany.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Faizan
authors.forename: Tuba
authors.initials: T
authors.lastname: Esatbeyoglu
authors.forename: Banu
authors.initials: B
authors.lastname: Bayram
authors.forename: Gerald
authors.initials: G
authors.lastname: Rimbach
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005399:Fishes; D006801:Humans; D015227:Lipid Peroxidation; D008099:Liver / Q000737:chemistry*; D008315:Malondialdehyde / Q000032:analysis*; D010659:Phenylhydrazines; D014481:United States; D014486:United States Food and Drug Administration
chemical_list: D010659:Phenylhydrazines; C004787:2,4-dinitrophenylhydrazine; D008315:Malondialdehyde
keywords: 2; 4-dinitrophenylhydrazine; HPLC; lipid peroxidation; malondialdehyde; polyunsaturated fatty acids