title: Triton X-100 as an effective surfactant for the isolation and purification of photosystem I from Arthrospira platensis.
abstract: Surfactants play important roles in the preparation, structural, and functional research of membrane proteins, and solubilizing and isolating membrane protein, while keeping their structural integrity and activity intact is complicated. The commercial n-Dodecyl-β-D-maltoside (DDM) and Triton X-100 (TX) were used as solubilizers to extract and purify trimeric photosystem I (PSI) complex, an important photosynthetic membrane protein complex attracting broad interests. With an optimized procedure, TX can be used as an effective surfactant to isolate and purify PSI, as a replace of the much more expensive DDM. A mechanism was proposed to interpret the solubilization process at surfactant concentrations lower than the critical solubilization concentration. PSI-TX and PSI-DDM had identical polypeptide bands, pigment compositions, oxygen consumption, and photocurrent activities. This provides an alternative procedure and paves a way for economical and large-scale trimeric PSI preparation.
authors.affiliation: State Key Laboratory of Heavy Oil Processing, Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao, 266580, China, daoyong@upc.edu.cn.
authors.forename: Daoyong
authors.initials: D
authors.lastname: Yu
authors.forename: Guihong
authors.initials: G
authors.lastname: Huang
authors.forename: Fengxi
authors.initials: F
authors.lastname: Xu
authors.forename: Mengfei
authors.initials: M
authors.lastname: Wang
authors.forename: Shuang
authors.initials: S
authors.lastname: Liu
authors.forename: Fang
authors.initials: F
authors.lastname: Huang
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D008958:Models, Molecular; D017830:Octoxynol; D010100:Oxygen / Q000378:metabolism*; D045331:Photosystem I Protein Complex / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D012995:Solubility; D013050:Spectrometry, Fluorescence; D053545:Spirulina / Q000737:chemistry / Q000378:metabolism*; D013501:Surface-Active Agents; D020524:Thylakoids / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D045331:Photosystem I Protein Complex; D013501:Surface-Active Agents; D002734:Chlorophyll; D017830:Octoxynol; D010100:Oxygen