title: Lipase release through semi-interpenetrating polymer network hydrogels based on chitosan, acrylamide, and citraconic acid.
abstract: In this study, a series of semi-interpenetrating polymer network (IPN) hydrogels were prepared as a support material for lipase immobilization. Hydrogels were synthesized via free radical polymerization in different compositions of chitosan (Cs), acrylamide (AAm), and citraconic acid (CA). The swelling values of the hydrogels were found to be 240-400%. Depending on the swelling results, Cs-P(AAm-co-CA)-2 hydrogel was chosen for lipase immobilization. Three different types of immobilization technique were carried out. Lipase release behaviors were investigated, and immobilization yields of three immobilization methods were compared, and the maximum immobilization yield value was determined for entrapment method.
authors.affiliation: Department of Chemistry, Faculty of Sciences, Gazi University , Teknikokullar, Ankara , Turkey.
authors.forename: Mehlika
authors.initials: M
authors.lastname: Pulat
authors.forename: Gülen Oytun
authors.initials: GO
authors.lastname: Akalın
authors.forename: Nilay Demirkol
authors.initials: ND
authors.lastname: Karahan
mesh_terms: D020106:Acrylamide / Q000138:chemical synthesis; D048271:Chitosan / Q000138:chemical synthesis; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000627:therapeutic use; D005650:Fumarates / Q000138:chemical synthesis; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry* / Q000627:therapeutic use; D008298:Maleates / Q000138:chemical synthesis; D011108:Polymers / Q000138:chemical synthesis
chemical_list: D003692:Delayed-Action Preparations; D004800:Enzymes, Immobilized; D005650:Fumarates; D020100:Hydrogels; D008298:Maleates; D011108:Polymers; C073341:citraconic acid; D020106:Acrylamide; D048271:Chitosan; D008049:Lipase