title: Methods for quantifying lysophosphatidic acid in body fluids: a review.
abstract: Lysophosphatidic acid (LPA) is a bioactive lipid involved in cellular signal transduction. LPA plays a role in both physiological and pathological processes. Elevated levels of LPA are observed in the plasma of patients with epithelial ovarian cancer, indicating its potential as a diagnostic marker. Quantification of total LPA can be performed by radioenzymatic, fluorometric, colorimetric, or immunoezymatic assay. Determination of individual LPA molecular species requires the use of capillary electrophoresis, gas chromatography, thin layer chromatography, liquid chromatography, or a matrix-assisted laser desorption/ionization time-of-flight method connected to an appropriate detection system.
authors.affiliation: Department of Laboratory Diagnostics and Molecular Medicine, Pomeranian Medical University, 70-111 Szczecin, Poland. Electronic address: annakarolina6@wp.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Jesionowska
authors.affiliation: Department of Laboratory Diagnostics and Molecular Medicine, Pomeranian Medical University, 70-111 Szczecin, Poland.
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Cecerska
authors.affiliation: Department of Laboratory Diagnostics and Molecular Medicine, Pomeranian Medical University, 70-111 Szczecin, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Dolegowska
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood; D001774:Blood Chemical Analysis / Q000379:methods*; D001826:Body Fluids / Q000737:chemistry; D000077216:Carcinoma, Ovarian Epithelial; D003124:Colorimetry; D057075:Enzyme Assays; D004797:Enzyme-Linked Immunosorbent Assay; D005470:Fluorometry; D006801:Humans; D008246:Lysophospholipids / Q000097:blood*; D009375:Neoplasms, Glandular and Epithelial / Q000097:blood / Q000175:diagnosis; D010051:Ovarian Neoplasms / Q000097:blood / Q000175:diagnosis
chemical_list: D014408:Biomarkers, Tumor; D008246:Lysophospholipids; C032881:lysophosphatidic acid
keywords: Lysophosphatidic acid; Methods; Quantifying